LOCUS       DAORGN010000001        68394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_114_length_68394_cov_6.312369, whole genome shotgun sequence.
ACCESSION   DAORGN010000001 DAORGN010000000
VERSION     DAORGN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 68394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68394
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114_length_68394_cov_6.312369"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..582)
                     /locus_tag="PKL17_00005"
     CDS             complement(52..582)
                     /locus_tag="PKL17_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK43131.1"
                     /translation="MTKELSFRIRSAEQDPAILSGLAEVTLDCVAGGASIGFMQPLSY
                     ERALSFWQAVLSAVARAERLVLIAEDLATQQIIGTVQLVLHMPDNQPHRADVAKMQVH
                     RMARRRGVGEALVRAIEEEARKRNKTLLVLDTVTDSDAARLYSRLGWQRVGDIPNYAL
                     FPQGGLCSTTFFYRAL"
     gene            624..1385
                     /locus_tag="PKL17_00010"
     CDS             624..1385
                     /locus_tag="PKL17_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43132.1"
                     /translation="MQSVRFGMGNTLALGILLGGTVASARECPAPKGSSPELASVLAE
                     DRIRFLARTLTDTARRERDYAIGWSLTYAGLSAASWVMLPLSSSDDPGQLVETAWNSG
                     TSAFAALYVLYAPLSAVRAVHKLNQRLASANDDVCAQLAEAERVFFKVADNQKGGRDA
                     LAHIGNVAFNVGLGLVLGYALHRPNGAAISTSIGVVMGELMIATRPSGALSTLADYQK
                     GRLLAPPTSPSLALPFQLVPTLQNQTYGLALIGSF"
     gene            complement(1394..1756)
                     /locus_tag="PKL17_00015"
     CDS             complement(1394..1756)
                     /locus_tag="PKL17_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNK43133.1"
                     /translation="MVKHIRKPTVIEAAGNKPKLIEEFFGRVNSQTDALSMARMRSPS
                     GWVEPGQTPEFDEYTVVLAGTLTVTTKEGSIDVHAGEAILAPKGEWVRYSTPGPNGAE
                     YIAVCLPAFSPATVHRDS"
     gene            complement(1809..3257)
                     /locus_tag="PKL17_00020"
     CDS             complement(1809..3257)
                     /locus_tag="PKL17_00020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNK43134.1"
                     /translation="MSFQLLQPIWVDLFVAGSALLLLLVDMYLPDAARKHLSTLTALF
                     LAVALYLTTQLDGTGSIGSVYVGSAFSVLVKQILLFATLLCVLGGKEWLSQTFTKKQG
                     EYHFLVLMCLCGMMLLPGVRDLILLVVSFELMGIPLYILAALSREESTPEKRQAVLRR
                     GAEAGLKLYLVGGASTTVTMFGLSLLIGSAGSTQLEALLAAPPSALSMMGMLLVLGGM
                     GFKIGAVPFHMWVPDTYQGAMTPMVTFLSVAPKAAGLITLTVLSQKAAPQALWQPAIL
                     GLSLLTMTVGNLMALPQKNVKRLLGYSGVAQIGYMLLALGVGNAYALGMLLFYLCGYA
                     VTNIGAFLVVHAVADQEGDDTLDGFAGLAKRSPWLGLSLLMFLLSLAGIPFVIGFWAK
                     LYVFMATYQAGLGWMVVVGAVLAVVGLFYYLQVARAAYMAKPTRSEPVQVEPWLAVTI
                     VVCLLLVVGMGVYPAPILKACQSAADALISAR"
     gene            complement(3254..4807)
                     /locus_tag="PKL17_00025"
     CDS             complement(3254..4807)
                     /locus_tag="PKL17_00025"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNK43135.1"
                     /translation="MTEHILSLIVAAPMVAALVVMMLPPKQHTLIRLVSALGAFLSLL
                     GSLYVAQLYNPQASGFQLSEVYPLVPQLGIDLRLAVDGWGVSLLILTGVIIFAGVLAS
                     FGLADRPKEFFVLLLVLVAGVFGVFVAQDLFVFFLFYEIAVLPMYLLIGIWGSSHKVT
                     EAGPLRFVWKWFDIGGREYAAMKLTLMLLVGSAFILVGLLALYFSSGAGTFDMAVLAK
                     AHYPKSLQIWTFVMFWLGFGSLAGVFPFHTWSPDGHASAPTAVSMLHAGVLMKLGAFG
                     VMRIGMMMLPDGAQFWAPIVGGVAVVNVVYGAMSALSQKDLKYIIAYSSVSHMGVVML
                     GAATLTQSGWNGAVYQMFAHGIMTGLFFALVGHIYERAHSREVAKMGGFATVMPGVAA
                     FFTLAGLSSLGLPGTAGFVAEFLVFAGGWQSPRPWWSICGVIGAFVTAVYVLRVSRAV
                     FFGDGPSDHFHDLRDVRKIEWAPLWILGGTLLLCGIWPRLLLDFIDPTTTNYLRMALG
                     SVLPVGVLP"
     gene            complement(4804..6609)
                     /gene="nuoL"
                     /locus_tag="PKL17_00030"
     CDS             complement(4804..6609)
                     /gene="nuoL"
                     /locus_tag="PKL17_00030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNK43136.1"
                     /translation="MFHSAYSLALIALLLPGAVALLLATVPALRHRVTAIVFSVLAAL
                     TSLGSAILLVLGQLTPTPPQLWTVPWLPQAGAPLAEIGLRIDGISASMLLVVTFVASC
                     VQIFSIGYMHDESKEAQGRYFTYHSLFLTAMNILVLAPNLLQLFLGWELVGVTSYLLI
                     GYYFKKPSAAKAAVKAFWVTKFADMGLLIGLLVLRSKAGSFDWDVQIAPSAANLVTLL
                     LFVAVMGKSAQFPLHVWLPDAMEGPTPVSALLHAATMVAAGVFLIVRGWPLFELAQGT
                     RQIMLAVGSVTALIAATMALFQNDIKKVLAYSTCSQLGYMIAALGAGSLLGGFFHLTT
                     HAFFKALLFLGAGSVIHAVHSNDMRDMGGLARKMPLTTVTFLIGSLSLMGLPGLSGFF
                     SKDLIIEAVGEHGVSWQLVFLLISAFLTAFYMGRLLVRVFFAQATEKASHAHESSAVL
                     ILPLLLLAVPALAAGYLGKMFGQLYGSAYHFHLGTTGIIAAGLGLLGLGLAYLLYRRS
                     DEHPALLAPIARLVEYGPMDRLYAGGFHRVALPFAALIGWIDRYLIDGIMNLTGLAFV
                     VSGQKIRRVQTGIATDYVLATVCGVLLLVLYGVLR"
     gene            complement(6622..6921)
                     /gene="nuoK"
                     /locus_tag="PKL17_00035"
     CDS             complement(6622..6921)
                     /gene="nuoK"
                     /locus_tag="PKL17_00035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006672536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNK43137.1"
                     /translation="MTLGLCLTLSAALFCIGLYGLLTHRHAIGLLLSLELMANATNIN
                     LVSFSRFRGDGIGQVFALFSIALTVTEVVVGLAIVILVHRAYRDAQVSRASEFKE"
     gene            complement(6918..7430)
                     /locus_tag="PKL17_00040"
     CDS             complement(6918..7430)
                     /locus_tag="PKL17_00040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNK43138.1"
                     /translation="MIEQVGFGLVAGCIVLAALLVARAKSAIHGVLWLGVVLGATAVL
                     YAMLKAPLLAGIQVLLYVGGVMTLMILGVMLTRHDQKEAALPHKKPYVAAVLALAMLG
                     MLVKAIQSTPDLPARDAPPASTQQLAKLLLVDHALSFEALSLLLLATMIGAIVLLRRD
                     RPAQISEDGR"
     gene            complement(7427..8233)
                     /locus_tag="PKL17_00045"
     CDS             complement(7427..8233)
                     /locus_tag="PKL17_00045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNK43139.1"
                     /translation="MGFFGDSFRALKTTLTNFLRPAVTVEYPEVIRPRPERHRVSFAL
                     LIDETGDEACIGCLACERICPSQVIAIKQAPKRESKVTGKKRGYAEDFTLDLEACIFC
                     ELCVQVCPTDAIVMCRAPEQPGYCREDLVLTMDKLYENQKQKPLSWGTGGKLMEMQDP
                     ARGKAKPAEAAGDEKSAEAGAEKPKRVAKAKAAKAAAEPAKEAAPAAAKETAVATTDS
                     AKEPAPATDAKPAVAAEAAAKQTAPASPVSAQAEQKTDTAEQKTDPKEAP"
     gene            complement(8236..9204)
                     /gene="nuoH"
                     /locus_tag="PKL17_00050"
     CDS             complement(8236..9204)
                     /gene="nuoH"
                     /locus_tag="PKL17_00050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNK43140.1"
                     /translation="MNALLHGILYGLPIITVVLTVTAIGLWFERKFAARIQSRMGPTM
                     VGPAGILQPLADVVKMLQKEHVTPAAADRPLFELAPILCGAFAVATAAVVPFSPSVVA
                     ADLDIGVLYVLAANALVLIPVWMGGWASNNKFALIGAMRAVAQSVSYSVPMVLSALVP
                     VILAGSMSLSDIVRYQIDHHWVACWPTLPGLPAFVLFFLSSLAEANRIPFDIPEAESE
                     LIAGITTEYTGMKFGLFYMAEYIHTLVASAVASALFLGGWDGPVFPGLHWMVLKTLLL
                     FASIYWVRWSLLRFRSDQLMTLCWKWLVPVSLLLVMAAAAFAKVGG"
     gene            complement(9201..10388)
                     /locus_tag="PKL17_00055"
     CDS             complement(9201..10388)
                     /locus_tag="PKL17_00055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNK43141.1"
                     /translation="MSLVQIKRQNPHLKLDLYEPEADLMVLNFGPQHPSTHGVFRMQL
                     YLDGEKIIKAVPDLGYLHRGVEKLCERLTYVQVTPIVDKNDYVSPMHNEQAINMAFEK
                     LLGVEVPRRARYLRTIFAELQRIASHLLWLGTFALDLGGAIGGGASVFLHCFREREMI
                     LDLFEEVTGARFHYNTHTIGGNRHDITPGFADGVKKALQVIESRLSEYESITLANKIF
                     SERTKGIGVLDGELALELGLSGPILRASGVDHDLRRDAPYHAYDEIEVNVATAKEGDC
                     WARSVVRLAELRESIRIVRAMIDAVPEGPICGYKPIKLVGQVKVAQGQVYAAMESSRG
                     ELGTYVIAGGGDGNASPYRLKIRPPSFHALSALPYLLPGHVVADAVVILGSLDPIMGE
                     VDR"
     gene            complement(10393..11571)
                     /gene="nuoB"
                     /locus_tag="PKL17_00060"
     CDS             complement(10393..11571)
                     /gene="nuoB"
                     /locus_tag="PKL17_00060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNK43142.1"
                     /translation="MAIEDNLPFSHPLYDFMMGVPGLSVATGQVDKLLTWGLTNSLWV
                     FPMATSCCGIELMATAASRVDIDRMGTIVRGTPRQADVMVVAGTITVKMAPRVKKLWD
                     LMPEPKWCIAMGSCAISGDFYRDLYPTVPGIDTFLPVDVYVPGCPPNPEALMAGMLRL
                     QEKVRLQRAGKWTQKETRPETAKMIMPALRRLGDPARDPKTDEAQLQSAMKVAGSDPL
                     LEAPTAPRPATTSDTQVSPDGDLEAMLRAHGVSEFPKDAPPLVPVDRHRDLAQTLKKK
                     GFRQLVTVVASHYPGEKDSEGNVKKPEQYEVMYGLRTTGKGSQLALWRVRFESPDGID
                     SLYGVFAGADWQEREQFDLVGVRFAGHPDLRRILLPHDYSGFPLRRDHAADAPCAPWR
                     "
     gene            complement(11562..11915)
                     /gene="ndhC"
                     /locus_tag="PKL17_00065"
     CDS             complement(11562..11915)
                     /gene="ndhC"
                     /locus_tag="PKL17_00065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNK43143.1"
                     /translation="MSDAWLALLVFLALSFVVPLSMAGAGWILRVRARKESPLKFQTY
                     ECGEDQAGPTWIQFHPRYYVLALFFVLFDVEAAFMLPWGVSVRDLGVVEMGIFVGILL
                     LGWGYAVKKGALRWQ"
     gene            complement(12184..13788)
                     /locus_tag="PKL17_00070"
     CDS             complement(12184..13788)
                     /locus_tag="PKL17_00070"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNK43144.1"
                     /translation="MTGKSTSFRLFRSLALFTTGALITVACTKVKVSWDQQASAAAPG
                     AAAPAAVAPLLPQTQAPSLPPPTPSQIADARSLSRTFSQVAEQVSPSVVAIRVAKKQK
                     IKVMRRGSPFGRELPFHFGPFGSPFGMPDDDGEDDGTEERQGPVQRGAGSGVVIDAKG
                     YILTNNHVVGEADDIKVQFIDGKELPAKIVGTDSRSDLAVIRVDPKDYSLKAARLGDS
                     EKLLVGEWVMAIGNPFGLDHTVTVGVISAKGRSGIGENRSNYQDFLQTDASINPGNSG
                     GPLVNLSGEVIGINTAILGPGGNIGIGFAVPSDMAKPVVSELIASGKVHRPFLGITMQ
                     PYEPDLAKAMGGPEKGALVQGLTGGGPAEKAGVRRGDIITKVDGKAIANSREVQRQVL
                     THRVGDPVALEIWRDGKMVTISAKAGELPSDEAPAQSAGSTDDANSARAKLGLALQPL
                     TPQLAERLGIKGGQGVVIGGVKPGSPAAEAGLHQGDVIAEVDRHPVKSVDEATKLLGQ
                     ARPGGHVLLIQRGDSSVYILIRPSTP"
     gene            complement(13970..14791)
                     /locus_tag="PKL17_00075"
     CDS             complement(13970..14791)
                     /locus_tag="PKL17_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK43145.1"
                     /translation="MSDAHPDMARILRVIERLQDRPFRTHVVIYGEPGTGKEGLAKLL
                     HRLMHPSGAPQVRAHLAGCSSEEMLKQFFGPDTYMSGDGAVCSRYEDGLLSKADGGSL
                     LLDELLVMPLDVQRSLHEHLQEHRLGTENGVALIALTDGNLPQAVRAGQFRHDLAYRL
                     LRIVLHVPPLRERPQDIAHSTLWICNRILRSFHDERPAELVDANSPASPDSVFLVEED
                     TLSLLKRHDWPGNFRELEAVLERAIMLYSNRERLRPEDVESALGDAWPPVQFRRS"
     gene            14990..16372
                     /locus_tag="PKL17_00080"
     CDS             14990..16372
                     /locus_tag="PKL17_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNK43146.1"
                     /translation="MQKRFFSSRACKTTLLGWVLGPIFSLCGVTAAAETSPVGPVPLT
                     PPALPTEPLRYSDEWVKKELTELHLRYPKLTKLVPIGKSHEGRPLWALAIGRNLKRHD
                     GRPAILVNAAHHGVETFSIDLALDVADVLLLRAGDDQRQGLRIDPGLDKKVQRWLKEL
                     VIWCVPVVNPDGVWASLHGFVRTGRKNGRDNNGNGKPDRGDGVDLNRNYPFRWGFLGD
                     KGSSPQPDSAYFRGPAAGSEPETQAIMRLAESEHFAASLSFHTGNVTVLAPYTIDNVQ
                     SPTPNEAWTVAEELVAGLPRHPQGKDIIVRRNIYPVDGTDQDYLRGQFGTLALLVEGA
                     RRDPTQAEARRNVVLAMRPIWTRLLDRYLDGPSVYGVVRDGKGRPVVADVSIVEQTMA
                     EQEHWQTRCRDGHYDRFLPGPGEYTVRVTAPGVAPKDQRIKVGKERLRIDFTVEAAAS
                     SGQCPKEPPG"
     gene            complement(16317..18668)
                     /locus_tag="PKL17_00085"
     CDS             complement(16317..18668)
                     /locus_tag="PKL17_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNK43147.1"
                     /translation="MRRASLLLYLSLSSVANASPGPSETIGPVPGYLGQHARTRGFSL
                     GKPMRPTPTPDGKQVLFLRATAASPELRLHVLDVATGQTRELLSPAQLLQGAEEHLSV
                     EEKARRERMRQSMRGFTSFELSKDGSFLLIPLSGKLYTYQLRTADSSEITPVTDPSER
                     SGEPIVDPHLSPSGKAVAYVRGRDLYVMDLATRRERRLMHSTNPVISYGSAEFVAQEE
                     LHRFSGFFWSPDSTMIAFTEVNNQPVETLYVGDALHPEQPPQPHRYPRAGTDNAEVRL
                     GLIALTGAAPQWVQWQRDKLPYLATVVWREGGPLLLTLLSRSQRDLELVTVDLKTGQT
                     QRLLHEHSDAWINIDQDVPRPLRDGSGFLWTSERDGSRELELRDYQGGLKRLVVDKSQ
                     GFRKLLHVDEQSGYISYAASNEPTQQDIYRVSMLSGVPLRLTQAGGYHAASFGQHPGV
                     YVLSSFSTTQLGKTVVMRVPESSQAAASDQVVGELPSVSTTPPFVPNLEVTQVGTQNY
                     RSLLVRPRNFQPGKKYPVLVHVYGGPHHNQVMAIAGMYMLDQWLADHGFIVFMADGRG
                     TPGRGTTWEHAIAGNFADIPLEDQVAALQAAGDKYPEMDLSRVGIFGWSFGGYMAALA
                     ALRRPDVFHIGVAGAPVVDWRDYDTCYTERYISTPQENPDGYERSSLLTYASQLRRPL
                     VLIHGTADDNVLFFHSLKLSQALFRAGKPHELLPLPGLTHMVPDPVVTERLWGRIVGA
                     FSAVLRPELAHLAVKPGAGAPPLPEPIPVVPSGTVQKKPQPRR"
     gene            complement(18688..19257)
                     /locus_tag="PKL17_00090"
     CDS             complement(18688..19257)
                     /locus_tag="PKL17_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43148.1"
                     /translation="MRPTENGQLFLSRVCDLLLSLPFDLKVLQEVITDVELPQNIREQ
                     AASVLVQTFGHQDGSGPERFLEDVFLLRIAFGNIAADKSEEADRFRSRFDDIFGTLDD
                     DLTLFSGYLGSELWNGLRARAANLGRLVYKGKRPAQYVADEASWDELYEDGLEFQTAY
                     NVSEEQVKNRLRRPEPICEAIQKRTARPK"
     gene            19380..21164
                     /locus_tag="PKL17_00095"
     CDS             19380..21164
                     /locus_tag="PKL17_00095"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK43149.1"
                     /translation="MDAIVLRRELACATAPSALFAVLADTDRLYRTLGQVAVSREPLS
                     GEGSARFLLRARGEAKAIPFTEIPPQWSHPSLLVTKRVLHQGFLASLATRFTLTPRMQ
                     GTQLLIEMQVEPRLVQLGWLVKLFAQATLWHLSRTILRIDDGIPRGEPTQFRPAQLAV
                     EPLRQAQQQTKTQLPPEEQSQVDSLVAHLLRTDDLDVDCLRLGGVSEALGVPESTALR
                     LLLLAASAQLVQFGFDVLCPSCRNPAAQVDHLTDLTDEAFCTLCELRIPVEFASNVEV
                     TFRPASSLRKVSRPLYSSVNPATAQHVITQLVLPGTTKLTMTVPNEPGEFRLWARGGA
                     TGTLRVTTLGPSQASVTVGDTMVPAEVEVAMGGTLEIDHQTGQARHFKLERPDWKQQR
                     LDGHQLTLHPLFRRLFPHELPKPSVQLAVPTVALWRCRFASLGELCAALGDSGAFRQL
                     QDCQQAMRKAVDEAAGAVLRSDSDGLTVAFASGAVALRCAIAVERALRTLRADNPFAH
                     LLLLKFGLYHGPATVVGVDKRLDYFGQTVAVCDRMVSEAHPGDILVPAELLSDVSGME
                     GIKAGPIFSLTGRGLSSPVAVSRLTVES"
     gene            complement(21148..22131)
                     /locus_tag="PKL17_00100"
     CDS             complement(21148..22131)
                     /locus_tag="PKL17_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNK43150.1"
                     /translation="MNMHAVVLPSVGPPEVLRFLERPRPTPGRGMVLVEMRATTVSPL
                     DCQERAGQVSRLIPGGRPEILGHDVCGIIAAIGEGVSKLTVGTEVIGLRALQNPGTYA
                     EFVTLPADAVTRKPMKLSFEAAAALPLSGTVALQAIRDRGRLQGGQALLVNGAAGGIG
                     SLAVQLGKALGAVVTGVCSGDSSELVASLGADEVIDFEKEDLATLTGTFDVVLDTSGQ
                     LTLDRCRKMLRPKGRFIPIVPSLRTLLDSVTSRFFGGPQTVPPPLAQPRRADLELLAE
                     LADQSKLRPIIDRVFPLAEAAAAHRHVEEGRPRGRVVLCIGSEKTPPKTQR"
     gene            complement(22183..22938)
                     /locus_tag="PKL17_00105"
     CDS             complement(22183..22938)
                     /locus_tag="PKL17_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK43151.1"
                     /translation="MVTDHRKVRRLDGDNLKQDLIGGQHAFSYLRHLVRGNEMTQRRF
                     PDQLSPNHPPVLLIHGFLGTRGSMFVLEQRLLRDGFHVFSFNLGMLNVRDIRTSALLI
                     HRKVQTICEQTGIRRIDIVAHSMGGLIGLYYIKRLGGKEQVRRIVMLGTPARGTWVAA
                     LGVATMGLLSASSWQLLPGSDFLEELHAGPLPNEVEFFTIAAERDWLCPPSATQLEGA
                     HGVTVPLGHSSLVMSEEVYRKVLWALRKDDSPR"
     gene            23355..25382
                     /locus_tag="PKL17_00110"
     CDS             23355..25382
                     /locus_tag="PKL17_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK43152.1"
                     /translation="MSALSQNPDSSHDELVALRVDRPTYPKTFLRSDERQSALALARA
                     IMPEAGGLPEAGSATVLRAENWLADVPMAGDGYRALLRGFDLYVRAATGKYLQSVDPE
                     RLRELCRRFAEGDIGRRYFLYTLTAPLKGAYFDDPAIYRHFGIPYRGAPSAIPQTLVP
                     KELPRWAHERTQSACEVSDGEVIDCDVVVIGSGAGGAVAAYELAKAGRAVVLLEEGQY
                     FGRRDFAHSAFHLQKHLYRELGVTMAVGNTFIPIPVGRTVGGSTTVNSGTCYRMPSRV
                     FADWHNHHGLTELSPDSMDPFYGAVEDVLGVKQAPRSLLQGNQPIADGCDKLGISHHG
                     PLARNAPDCDGQGLCCFGCPTDAKRSTNVSFVPMALQAGAQLLTSAHVDRVIIEHGRA
                     VGVSVVSTQPAGSGGRPRRFSVRASAVVVAAGSLMTPVLLLSDPVTRRALGRSGALGN
                     HMTIHPAIGLRAVMPTKLNSHIGVPQGYAVEEFHDEGILMESAFLPLELTAASITLVG
                     KPLMELMGAYDRHSCFGFLVEDKGQGSVRPGPGGRPLIHYNLADRDVARLQRGASLVA
                     RMFLAAGAERVLTGISALPMLSSHDEVDRLSGLRLRPRDFELTAYHPLGTARMGHSPQ
                     NSVVDTDLQSHDVPGLYICDGSVIPTSPAVNPQVTIMALAMRAARRLAERL"
     gene            complement(25333..25944)
                     /locus_tag="PKL17_00115"
     CDS             complement(25333..25944)
                     /locus_tag="PKL17_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK43153.1"
                     /translation="MFTLKHVAVALGLTVAALSAFVASRPDAFHVERSASISAPADVI
                     YTQVGDFHNWNAWSPWDALDPSMKKVYEGENNRPGASYFWEGNDKVGSGRMTIVDANP
                     PSQLSIKLEFIKPWPGTNDTRFAFTPDGDKTKVTWSMSGKANFMMKAMTVFMNMDKMV
                     GGDFERGLKKLGEVAESAHARRAAEAKATAVPPVAAPPASPAP"
     gene            complement(26017..27831)
                     /locus_tag="PKL17_00120"
     CDS             complement(26017..27831)
                     /locus_tag="PKL17_00120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43154.1"
                     /translation="MSVSQEQKEAEKLVGQVLNGTYRLEQLLVTGGMGAVFRARHVRT
                     GGVCAIKVLHQRAAMQGDLSERFQDEARIIAGLRHPNIVQVSDLDRDRSGMAFIVMEL
                     LEGEDLQDRIERTGKIPLEEALQITRQISSALHAAHERGIIHRDLKPRNIFLSHHEEH
                     GEVIQTAKVIDFGVSKVPRPADKATKDLLVLGTPRYMAPEGAMGQNSQIDGRSDQWSV
                     AVILYRMLSGKLPFDHENVIDLFKQVINESPTPIEQLEVTVPPHVAAAIKRALSKKKE
                     DRFPTMLDFLRALDPPPAAPPFSWKRSLLFGLAGAGMAGLFVMPLLVRELQGKKRSGQ
                     VGQMLVMADEALMEERWEDAVRLATQAQSVPGQPPYVSTAAENIKQRVERGKKLSSEV
                     QQIMQALASKDYDGALSRNRELPPRTSFRITAQQHYDLLQQLVVAYHLQQAEAERSLG
                     RCDKIEPHVAKILEVAPDHVDALTARWRPCALITGQPPGAKKLAGYPPLRDQDGKPLG
                     NAGEYDKQRNNFAEIDRYLRDAQIAHQHGEFTKAVALAHTSTSVPIRSPLAWRIAGVS
                     ACHLQSRDILEYVVPHLDPASKEFVRGRCTALGYKVWF"
     gene            complement(27891..28334)
                     /locus_tag="PKL17_00125"
     CDS             complement(27891..28334)
                     /locus_tag="PKL17_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43155.1"
                     /translation="MDDTRPRDASTAWVSIGPHRFAEYERHCEWQPHGVVALPHAEEL
                     VKRLLAYQERTGKVALIVDGRELAPLSAEVRNFYVQAVNRGLNYMPIVVYGASLLAKV
                     TQTLALRAGQIIHQLHIELRHVETRQEAVAYIQKRLNIHLRESLS"
     gene            complement(28377..29639)
                     /locus_tag="PKL17_00130"
     CDS             complement(28377..29639)
                     /locus_tag="PKL17_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43156.1"
                     /translation="MQPTPTKGVNETSQAPHQIDRLPMPMLVFLLMVSLWDGFDVVAL
                     SQTLPELRKSFALSVSEGGQLVGLANSGTLLGYFLLRRADSVGRRSILLSSVLGYSLL
                     SLTSALAPSAALFLLTQFAARIFLVSALGAASLYISESLPARKVRSALSLLVATGAFG
                     GVVCALLAPKLMGTGPGWRSVYLLGAVMLLTVPWGYLHLHETPSFARLTSRVTPSLTA
                     MWTVQHRRNLLLCSFLWLLTYAANQSTITFWKEHAVADLSLSAAVAGTYLGLAALVAI
                     PMSGFVGPLLNRVGKRRGALIVYGLWTLGILGSYLLPAGPLLRLALACLTSGASGGLI
                     IATAFTTELFPTAQRGDAMAWSNSLLGRIGFIASPPLMSMLADAQGWTNTMPYLAVLP
                     LSALVVVWLVPSRRPPPRDDNAPEWSGD"
     gene            29750..30619
                     /locus_tag="PKL17_00135"
     CDS             29750..30619
                     /locus_tag="PKL17_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK43157.1"
                     /translation="MRLSAVIGNSQRLDGGAMFGNAPKALWTRYFAPDSENRISLACR
                     ALLVEVGARKILLETGIGVFFAPEMRSRFGVVEEDHVLLRSLAALGVAEDEITDVILS
                     HLHFDHAGGLLSAYVGGQSARLLFPKARYVTSASALARAEKPHVRDKASFIPELQGLL
                     RASGRLHLIADGATHDETLPGLRFHHSNGHTPGLLLTEVCGPSPDRAVVFAGDLVPGT
                     AWVHLPMTMGYDRYPELLIDEKTALLNQLCDRGAWLFYTHDPVVAMSKIRRGEASRLS
                     ADKPETSPTRFVL"
     gene            31024..31380
                     /locus_tag="PKL17_00140"
     CDS             31024..31380
                     /locus_tag="PKL17_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43158.1"
                     /translation="MASRVPVPPRSFDDQLESLIAFLKQSKTALVGVDPKQLESDLKA
                     QREERQKDHDAERQYEQIHRRFLESQAERYRRFGVAVQVMRAANRHNADVLAAMEQFK
                     RPRTATSAKKSKADKP"
     gene            31428..31703
                     /locus_tag="PKL17_00145"
     CDS             31428..31703
                     /locus_tag="PKL17_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43159.1"
                     /translation="MREALGFGLGMGLATSYVLLLRLNARLYTQNSGQGLALLLHVGR
                     LVVLVAVMVLLGSLGKRCLLYGLLGFVPTHLLLYLWLRRQPDDIAPP"
     gene            31684..32373
                     /locus_tag="PKL17_00150"
     CDS             31684..32373
                     /locus_tag="PKL17_00150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNK43160.1"
                     /translation="MTSPLLEPALFNIGPVPIPASLCTSVGITVVLGAGSFILSRLLR
                     PEPGKVQAMCEVVITTILEQIEEVTRKDGRPYLPLIGTLFLFLIVANSLSLIPGLQPP
                     TERLETVIALSLTVFVSVYYYGIRRHGLFSYLKHFFQPTPLLFPIHVLSELTRTFSLS
                     MRLFGNIMSHGLILAVIVSVAALLVPIPILLFGLFTGAVQAYIFTILATVYIAAAVED
                     HPQAVKTGHKE"
     gene            32382..32624
                     /locus_tag="PKL17_00155"
     CDS             32382..32624
                     /locus_tag="PKL17_00155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNK43161.1"
                     /translation="MSVQVVSILGAVIAVCFGAIGPALAEGRAIQAAMEAIARQPEAG
                     PTISRSLFVGLAMIEAMAIYCLVVALVLLFANPFVK"
     gene            32628..33368
                     /locus_tag="PKL17_00160"
     CDS             32628..33368
                     /locus_tag="PKL17_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNK43162.1"
                     /translation="MHVSLLHIAFQTINFLVLVFLLQRFFYKPILGVIAERKQKIARE
                     QELAASEKQAAEKLKADLQNKLQAIEDDKQRVLQQALVQAETQAASLRREMEQKIDGE
                     RARQTALLQSERSEVEQKLKEQSVRLGLLIAERLLREGAVVSDPLVAVHRALGQIEAL
                     PSSEQGRVWTELRQHGAELTSVDALSVTQVAQVKAAIESACRHPVSLTTQTEPKLLWG
                     VELRLGHLSFAFHLHAGLAKVQSELLTP"
     gene            33365..34906
                     /locus_tag="PKL17_00165"
     CDS             33365..34906
                     /locus_tag="PKL17_00165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNK43163.1"
                     /translation="MSDLLRNLIAFAQDTEGKVRELSLAPRPESLGRVIAIADGVVHV
                     SGLPEAQLGEVLRFASGTMGLVVVLDEDQIGCVLLGKQEGILAGSSVRGTGQVLDVPV
                     GEELLGRVVDPLGLPLDGGPKIEARHHLPIERPAPGITQRQPVTEPLHTGITAIDAML
                     PLGRGQRELIIGDRFTGKTAIAVDAILSQNSTDVICIYAAIGQRASSVSQAVAAIRER
                     GPKSRTICVVAASDAAAGLQWMAPYAACAMAEYFSERGGHVLLVLDDLTKHAQIHRQV
                     ALLLRQPPGREAYPGDVFYLHSRLLERAAKMSTDRGGGSLTTLPIAETLAGNLSSYIP
                     TNLISITDGQIFLEPRLFNEGQKPAIDVGKSVSRVGGKTQLAVMRKVSEKLRLGYAQF
                     LELEVFTRFGGAMDERTQKVIERGRRVRAALLQPQFQPLSTAHQVALLQAIDDGLLDG
                     LPTSAVGLYRDALGTLLAEEHKLLALRIEQGSVLSEPEQKQLGEVLRAALLRVNANEG
                     KDVRG"
     gene            34893..35750
                     /locus_tag="PKL17_00170"
     CDS             34893..35750
                     /locus_tag="PKL17_00170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNK43164.1"
                     /translation="MFGADLAARIRTVQQLGDLTQAMRQLAAARQRQAAENFAGLERY
                     AAATLSALQRGLALLSKQQSAPKQSGTSQRLLVVCLSEHCFVGALNETLVSKVVHVMA
                     EAPSQLFVLGKRGLRHVEERGLSAHETASMPTTLGSVSRTASDLVERLFDRVSSGEVA
                     EIEVVFARLRGSVSYEPICQRLFPPHVLPAAYDGEPPLHTLPVEKLTARLLEEYVFAQ
                     VTWMIASAFACEQAARFSAMDASHRNLQEQLGELRQQERAERQDSITSEILEVATASS
                     LLAESHHDK"
     gene            35740..37122
                     /gene="atpD"
                     /locus_tag="PKL17_00175"
     CDS             35740..37122
                     /gene="atpD"
                     /locus_tag="PKL17_00175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNK43165.1"
                     /translation="MTNDGVIVRVRGSVVDVRFQSGELPAIGEALTVKLGGRTLMLEV
                     QQHSDVGQVRCVSLDYTEGLRRGQSAQRTGACIHVPVGENMLGRLCNVLGAPIDNLPP
                     PAEATPRRPIYRAAPPLRTEGQAPELFFTGIKVIDLLLPLRRGGKAGLFGGAGVGKTV
                     LLMELIRATVQTHGGVSVFAGIGERSREGNELWLDMQQSGVLKNTTLVFGQMNEPPGA
                     RWRVGLTALTVAEFFRDERKRDVLLLIDNVFRFIQAGGEVSGLLGRLPSRVGYQPTMA
                     TEVAEFEERITSVAGAAVTSIQAVYVPADDLTDPAVAQLFSHLDASLVLSRKLASEGI
                     YPAVDPLASSSNLLDPRLVGVDHYRIAEAVRKTLAQYRELQDIIAMLGLEELSQEDRL
                     TVARARRLQRFLTQPFLVTEAFTGRGGRTVPLAETLSGCKAILAGECDAWPESAFYMV
                     GALDEAKNRL"
     gene            complement(37132..37482)
                     /locus_tag="PKL17_00180"
     CDS             complement(37132..37482)
                     /locus_tag="PKL17_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43166.1"
                     /translation="MQAEQHALTAQPGLRSTRVTSFAVRNDIRAISLGYRSGTLQKYS
                     GIFISAVAWIVFLIGLAYFLLAAMQQQAEQLGPGLDSTERGISYCLSAILLLAVAVPQ
                     WVMGERRLSRLLAT"
     gene            complement(37634..38593)
                     /locus_tag="PKL17_00185"
     CDS             complement(37634..38593)
                     /locus_tag="PKL17_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNK43167.1"
                     /translation="MSTLGLLLSSSVFRLPSKESTAHAAPATSSTMPIAKPVPVQSQP
                     HPVASVGLEHPPGVRHVVIISEDGMRPDLIYGQNMYWHEQLYRNGSFTWKARTIRTAS
                     TLPSHASMLSGVDVDQHGLTWNTWLKTRGYIRVPTIFQAAEEGGLKTAAFVGKPKLRH
                     IMPEGTVDVFARPGYYCKKVSDEAARYLTKEKPGLLFVHFSDPDEAGHSDGWMTASYR
                     KAALASDACLGTIYRALQEANMLDDTLVIVSADHGGHARGHTGALPSDREIPWIASGP
                     GVRRGYHIRSNISTMDTAATALAVLGLSPLTRISGRPVAEIFQ"
     gene            38771..40069
                     /locus_tag="PKL17_00190"
     CDS             38771..40069
                     /locus_tag="PKL17_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43168.1"
                     /translation="METGETNRGGDASRHLASEPRLRPPVGGSYLGLLWHNRDFRAVY
                     LASLISLGGDWFMMVALYDLVLSQTGSATLISVINLCLGLPALLVTPWAGSLIDKTDR
                     RKLMVTVDLLRAGLALIPILVVSPKLLPLAFLAVACLSAGSGFFDPAAEAAAPNIVAP
                     EDLGRANALLGSAWGTMLMVGSAVGGLVATYLGRNVAFALNGLSFFVSAVLLWRIKLN
                     FSEHSHHTAPQTTLRESLSEAVTFVKSRPQVMALLSGKGLLGLTLGLTSLLSVFGEKV
                     FLRGSEGIAKLFVARGLGAVVGPFVLFALLQRSSLRTLAIGPCMMVYSLGYCFLSKSP
                     SLEWAMVPIVLGHMGGGAMWQATTYAMQESVPDALRGRVFSFDQAILGVTYSLSTMGV
                     GLLVDRQGARPVFFVLSLVFTLLGLLLFVVTRRLWQTHRV"
     gene            40128..40646
                     /locus_tag="PKL17_00195"
     CDS             40128..40646
                     /locus_tag="PKL17_00195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK43169.1"
                     /translation="MKPTLSSAKPAETSSRRPVSGRPVSGRPASGRPVSGRPLTVRET
                     RAHVPSGPTRVEKRRSMRFDKAFPVALRSDAFGEISAIARNVSTGGMMVEVREPLPLG
                     TQVLVYFSLPDSHVRVTARGEVKNHYFLNFADGKGGVRALSGMGLRFSSFESDSDLTL
                     GMGINRLRTVLH"
     gene            complement(40654..43143)
                     /gene="mgtA"
                     /locus_tag="PKL17_00200"
     CDS             complement(40654..43143)
                     /gene="mgtA"
                     /locus_tag="PKL17_00200"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNK43170.1"
                     /translation="MLRSVDERWKLPAAKLLSELASSEQGLRESEAKARLAQWGENTL
                     QTAKKLSVFRLLFEQIKSPLVLLLVFASGVSLLAGEWVDACLALLIVAASSAIGTWRE
                     FDAGRAIEKLKSLVVAKARVLRDGQSVQIPSSQVVPGDVVLLAAGSLIPADGLLLLAN
                     DFFVSQALLTGESLPVEKHIETEPQLCPPLQRKDAVFQGTTVRSGSATFVVLATGRKT
                     FFGSIADKLNLRPPETEFDRGLRHFGNLLIVTMLIMVLGVVVVHVALHRPLFESLLFS
                     IALAVGLAPEMLPAILSVNLSQSAQAMSQKGVLVRHLNAIENFGAMDVLCTDKTGTLT
                     VGEVRLSGSYDPEGNRSTSVLTEAAINAHFETGLTNPLDEAIDNAAQLDPTGIEKVDE
                     IPYDFQRKRLSVVVREKDQLRMITKGAIVPILDACSLSESERTAHLERAEAWGQTGHR
                     VLGVAKKLTAPKGTWHREDEVDLSFCGFLTFFDPPKEGIGQTLSALKELGVSLKVISG
                     DSRAVNVHLAREVGLKAEEVLTGAQLDDLSDEALWAKVQTTEVFAEVDPNQKERIILA
                     LKKRGHVVGYMGDGINDAPALHAADVSISVDSAVDVAKEAADFVLLESNLDVLRRGIL
                     EGRTTFANTLKYILTTTSANLGNMLSMAATSIFLPFLPLLASQILLNNLLSDVPSMAL
                     AGDAVDPELIQKPRRWDMKFIRRFMVQFGLLSSLFDFLTFFVLLRIFQAGETVFRTGW
                     FVESLLTELVVALVVRTRRPCFRSRPARSLLGLTALITGIDLLIPYLPYAQLFGFAPL
                     PLPLLAVLVGITVMYVLATELLKRFFYRNAT"
     gene            43397..44881
                     /locus_tag="PKL17_00205"
     CDS             43397..44881
                     /locus_tag="PKL17_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43171.1"
                     /translation="MSEASNPERGPVAAQTLDGGWFRKERERIAVGRKGIALRLGSTE
                     SKVTTLETRKHDVPTEWFGVLRELGFRFPADVAQAETPVASVVTDVVQAAAIPSPPSD
                     VPVVIAAEFAVSASDPPSASADAVIEAVVIEAVPTRADIPTTAETATPVSPVIPAIPQ
                     AEMEARREEKTPNVEPVAEASVPQDAQTLVPSNHSEPLASASLTPFCGHWMRERRRER
                     GIRTQDLVTQLRTLPADLYTIERHNIRLPLRWIPGLLKLGLLTVDEAKVATRGPGSKL
                     SPMNGLWLRQQRGQFKLSPQAVGAKLGVSALDIRLIEARQWSLPSEWFPALKSLFTAP
                     ASATSKKVASKPSGSAAIAAVKKDSAAKAESKTKSTPAAVATGKTTKAAPAGTSAKKV
                     EAKAATKTEASKSPAKAPAKTEKPSVSTATAKVAAPAAKPVPSGEPGLAETIVSYRLM
                     LGQHAGLSAVDVLAQIAADLQLAKGKDALTFEKLRAAMKVLTSR"
     gene            complement(45015..45737)
                     /locus_tag="PKL17_00210"
     CDS             complement(45015..45737)
                     /locus_tag="PKL17_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK43172.1"
                     /translation="MTKLRNKHYYDEFSATYERHRHDGYHAFIDELTCELVGRYMNKD
                     TRLLEAGCGTGLILNRLTQHAKVAVGVDLSSGMLRKASARSLQVVQGSVSELPFADAS
                     FDVVCSFKVLAHVEPIEQTMREMTRVLRPGGYLLAEFYNTLSLRYLIKRLKRPTAIAA
                     DINDEAVYTRYDSLPQIRSYLPNEIQIETVHGIRVVTPFSQAHRWPLLGPALRMVERQ
                     ASVLPGVRRLGGFLLVVGRKET"
     gene            45949..47478
                     /locus_tag="PKL17_00215"
     CDS             45949..47478
                     /locus_tag="PKL17_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43173.1"
                     /translation="MFGVLTNSLLVSSLLIGQPGEVAAKKGKSKVGDKRKQRGDKPQA
                     TPAVTPPGAASNGPLAAVRLACDLGSSNPLCETAFHAASTAAGRRYTLVDSAKLEALF
                     AREPSLRGCRNDSCRTAIAEQLGLWRLIDVIVHSPRQKDIVASVSIFDPAARGIAADV
                     ERESKRDPAKLDSTIGEAVEQVMATQRLTAQLRLDVRPTDAKVKITDSRGSAREISEP
                     ERTGKSPIRLFLGGYTVHVEKPGFLAQDVPVTVAQTGASLQIELQLRPIEVRFEWSPK
                     DAIVRVDNRPVGAAYPVMELSEGPHRVEVLAPPGQPYESIVQDIDVRRDMEPVRLLLQ
                     RLTELRVQAPLGYSVSVDSQILPPDRFRAKGLQIETSHKTTPGLHLVSATSFRGLRLS
                     RQVEVRPGSSADAVIHPPALWPGALLATAGLLTAGAGIGLYFYDGRCLDTDCLFVTNY
                     APASYAMMALGGAAFLSGVIWLGYNAAHHPRFYNPQSQRVTIVPSLAPRYSGVLTSIN
                     F"
     gene            47513..49111
                     /locus_tag="PKL17_00220"
     CDS             47513..49111
                     /locus_tag="PKL17_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43174.1"
                     /translation="MSVRLARALSALVFGSALAGSGCAAPGGAICGDDRSCGASEQCL
                     LIGDTEACHRTCTGANGCDDNSPVCDAALKVCRSCFAGEDSLCAARSAATPRCLKGRC
                     VACRPPGDVASESSECGVSSSVLSPSPVCDPRSYSCRPCLRHSECASGICVKDGSDVD
                     FGIPRGACVPTKQVLVVDQELCSGTGPAFCTLRQAINRIDADHRYILLKKGAGATDFG
                     DLQIGTQASHKGLTIHIVGPLADIPPETATSEPRAVLGGAPLKDGLTVTQSSVFIDGV
                     YVRGNRAGLVCIGADAKLSVNRSYFAGNDTAIVTSTNCKLSLGDSWLGRGPRASVFGD
                     APGNVRGIDVAGGDFSVVNTVFSDNGDFRQDGFGGIRIRSLSAGVRRSTVVNSTLYQQ
                     SGLLKLGKYFTSVMCDVPVGDRLVLLNTLMLSDKPLLTSPEEHYVDSTCGAMVSHVGS
                     NDAWLNAANSVVLPMEAMPLRNAAARDLRLPATPGSEGPVLQHGGVPMIEVGGERILA
                     PSFDLDGRSRPTGSVAIGAFEPVP"
     gene            complement(49113..52259)
                     /locus_tag="PKL17_00225"
     CDS             complement(49113..52259)
                     /locus_tag="PKL17_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNK43175.1"
                     /translation="MFSALIRAALRSRAAVLIATLLCLIWGLFAFRELTIEAFPDPTD
                     TQVNIISLYPGQPAEELERQVSIPIERAVNGTFGLVKVRSINLFGLSFVTLTFADGVD
                     VLFARSQTLERLRLAELPPGVTPQLGSLSTPIGEIYRYSLRSVGKQGETIQGDPLLLR
                     TLQDWVVRPRILQVDGVADVVSYGGLVREIHVRADPALLAAKGLTLRDLEESLQGASV
                     NASGGVLVRGAEQLIIRSEGLFASPIDIAKVAVTTRGGTPILVKDVASVEEGWTPRQG
                     IYSRGHDTDAVEGIVLMRRGENPSAVLARVRAKIDDINARALPENVRVWPFYDRTELI
                     NKTLETVGRNLVEGAVLVLIVLLVFLLDIKAALIVATTIPLSLCVAFIYLYSRGMSAN
                     LLSMGAVDFGVIVDGAVVIIEHIVSTLQHPPKDAPEDNLERISWAVRQVARPTIFALL
                     IISAAYLPIFLLQRVEGRMFAPMAHTVVTALLGSLLFSVTLVPVLATLAYRKGSTHRE
                     SPVLKAAEALYLPTLRGALRRPLVVIGTAVASLFLAGYILQNRGSEFLPEINEGSLYL
                     TFTLPSNVSLDEGRRLVPRLTSLLQGFPQVESLSSQLGRPEDGTDAALPSNLEFFVKL
                     QPMDSWPKDTPSLGTLIEKMHASLAVVPGLSVNFSQPIRDNVNENIYGQMGQIALKIY
                     HHDMKDLQQTAEAALAAISEVRGVSDLGIVKSAPVPQLQIKPRRELLGRFGLTMSDVQ
                     HFITVALGGSPVGTLWEGERNFDVVLRLPEAARDTIEKISALRIPTKGGALVPLAAVA
                     IIHVDYGRATLNRENGRRYIGLRMNVRGRDLGSFVLDAQQAVSSKVKLKPGMTVEWGG
                     EFESKERSMARLRLVVPVALLVTLALLFKAFDSMALALLTLLNVPFALVGGAIGLWLV
                     DMPVSIAAAVGFIALVGQASLNGVLVVSSIEERRRHGEPLDEAIIQGSHSRLRAVLMT
                     AALAALGLIPAALSRAIGAETQRPIAVVIVGGTLSAALLTLIVLPVMYRILLRVLEAR
                     GTMAPPLTPGGAPSQPPP"
     gene            complement(52263..53540)
                     /locus_tag="PKL17_00230"
     CDS             complement(52263..53540)
                     /locus_tag="PKL17_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK43176.1"
                     /translation="MSKRVSHLPPTHDDEPDESGGAEESGDGHSPRADLGFAKPASSQ
                     RSLLVGFVAGALTASLGFFVLQSIKGQHQKTSPPTPFRLTKTDIVLSEGTPQPIPFET
                     GEVTIGEPLPLPPITARIAAVESRTAPSYAPMEGRLDHVAVKLGDHVEAGAKLALIRS
                     GDMATLLRELRASAALAQTKKALAERMQVLVEARGASANDLLVAKNDLRDAELQAQTA
                     DSRLKSMSIAPDADNRYWLLAPRSGTVIQVDSATGQQVGPGKDRPVVTLADLEEVLVL
                     ADVAQPDIGNLHVGDPVDIRIPGDNDSLGQGQVENISAVVDMDRQTIPIRIRAKNPSG
                     RLRPNSFVEAHFGKSPTKAPSVLRVPSASIVSDGLDAVVFVQTAPGHFKRSEVTIGRQ
                     RGGQTEVRSGLRVGDRIVVRGALLLLNALDVED"
     gene            complement(53533..54816)
                     /locus_tag="PKL17_00235"
     CDS             complement(53533..54816)
                     /locus_tag="PKL17_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK43177.1"
                     /translation="MFAPFLTAPRASAQSPPVESVAQEEFHPTADLDLPSLLRVVRDH
                     APTLQQDHLSVDLSQADARQSRLIDNPVLDAAVGTIPIGTPNPPDLPHPLRNIPNYGV
                     GLSVHPDLARRSAKIDRAAFLVTAADAQRRSSVRGQAIQLLRTLGDLAVASLRLSADL
                     RLTAQSKAALQLARERVKTGFGPPLDADRAEIELLRLEQQIAADQGDILTAQAACGQF
                     VGSRCAAFSDEQEARKFLARWIVRAETLSPQIEARPDLQALEAQRSAALAEDRQAYAL
                     RIPDPTVRVGYLYDSFVASGNQQHSVSVSLSTPLAMSDHGQASRQAAQSRARRFAEQK
                     RLTVLSSQVRIDSLRRALLTQRQRLTTLQEQVLPRGQAILRDVRRAFEARAVPLTDLN
                     QAQRALDELLLQEATTLGDLFRISLDLIQEGASDE"
     gene            complement(54985..56493)
                     /locus_tag="PKL17_00240"
     CDS             complement(54985..56493)
                     /locus_tag="PKL17_00240"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HNK43178.1"
                     /translation="MNESKQTESPKAEAEGVFSTGMLTSVRKLSGPLLFFSALSLWAC
                     DKGTMMPDPGTQPDPLTDLDRYVKTKDPSYSWKLRMTDKGSGYTAYFIDMKSLTWRTP
                     QEVDRTQWQHDLTIIRPDNLSPGPAVLAISGGSNGTPPTKAPDDMVQIAKATNALVIN
                     LSQVPNEPLTFANHDGKSHTEDGIVAFSWAQVMKTQDPTWTARFPMVKSAVLAMDTTQ
                     EFLRTSEGGNLNVEKFIVAGASKRGWTTWLTAAVDSRVAAAIPIVIDVLNVDTFMRQH
                     VETYGFWAKALYDYHYNHITSHIGTPEMAFMLKNEDPYLFRKRLTMPKFVINASGDQF
                     FLPDGSQNYWDDLPGDKYLRYVPNADHSLDGSDALESVLAYVTGFRDDRPRPTFTWKL
                     EGANTIRVQTQDKPQKVLLWQATNPKARDFRVETFGKNWKSSELFDQGGGVYQATVTA
                     PAAGYTAFFIELAYPSGQIVPYKFSTQVRVVPDTRPFAGIDPKTAKYEVAGP"
     gene            56570..56899
                     /locus_tag="PKL17_00245"
     CDS             56570..56899
                     /locus_tag="PKL17_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNK43179.1"
                     /translation="MKTIILLALSNVFMTFAWYGHLKWKSAPLWQAIVVSWLIAFVEY
                     CLQVPANRLGHGTYSAAQLKTMQEVITLCVFCVFSVTYLKEPLKWNYLVGFGLMALSV
                     FFVFKKF"
     gene            complement(56913..57863)
                     /locus_tag="PKL17_00250"
     CDS             complement(56913..57863)
                     /locus_tag="PKL17_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK43180.1"
                     /translation="MTRYSQSKQWLKDRFVNPPDWPARPGFREVLRWYKSRPPKQSGF
                     VPPSCPNDGRALRENRKTATVTWIGHATLLVQLDGLSILTDPMFAMRMFTIKRLAPPG
                     VALADLPPIDAVVVSHNHRDHLDESSVRALGPSVQYVVPLGLAGFFRSRGLPRVHELD
                     WWQDVTLHANGGTVRITLVPAQHWSGRGLRDHNQSLWGGFVIESPTTRMYFSGDTGYP
                     AAFAEIGRRFPNIDYALLPIGAYAPRWFMSPQHIDPEQAVIAFGELGAQHFVPMHWGT
                     FQLSDEPLDEPPRLLRTAMGTQADRILDLAIGETHFAKHS"
     gene            58130..59590
                     /locus_tag="PKL17_00255"
     CDS             58130..59590
                     /locus_tag="PKL17_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK43181.1"
                     /translation="MLASRLWPDGPIPVCFEELDPAHSQERGWVRSAVENTWQSLSSV
                     RFSGWGKCSVGTKGIRIRSQDVGPYTLGLGTEIDGKTDGMSLNFAFTTWSPGCQRTRE
                     FCITAGAVHEFGHALGFAHEQNRSDTPSWCTSEQGSDGDITVGSWDLSSVMNYCNPRW
                     TGDGKPSETDKQGVRASYGRGSTYWQVAQSFGTSFAVGDETQDPASSGADDAAFVDVT
                     GDGLSDLVLWFSASRTLMVAQSQSGGRFASLKTWSKGEGSATDRLLFADVSGDGRADA
                     LFVNRSTGEWSVSVSSGTAFRTPSLWKSGHGIGSTSQLAADVTGDGRADAIAFFSATG
                     NWWVAASTGLLFGGYSQWKSGHGIGSTSQQAADVTGDGRADAVVFFSGSGSWWVAPST
                     GSAFSSYSLWRSSHGAGSTYQWLSDVTGDGKVDAVVGYEKTGSFFVAPSLGNSFGTDA
                     RWLSGFGAALVPGINQVLVGDATGDGRADLLLRWRK"
     gene            59729..61183
                     /locus_tag="PKL17_00260"
     CDS             59729..61183
                     /locus_tag="PKL17_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK43182.1"
                     /translation="MTTTQPLPYDEIPMLPDGLGLGPTLLFRRDRYAFMMKLSTGAPL
                     CRVQLFDRKVALVTGALEIQQLLVENAGSLAKSAVQKLVGYPVLGDGLLGSSGELWRK
                     QRKLMAPIFTPAQIAGYGTDMMQCAAREMATYVDGSLLDVTQSMTRLTMSVAGKTLFG
                     ADTCSEADEIGRALAVAIRLFGKLSGSPLSFLQVAVKDGLSRLSQHLPDALASFVDKG
                     VQQLESPILFGQESADLDQAVALLDKYVADLIAKRRQSPDASHDLLTRLLAARDGADQ
                     MTDKQLRDEVLTLFVAGHETTAVSLSWSLYLLAKHPEIYSDVQQLVDSLPAEPTVADL
                     PKLGKVLRVFKEALRIYPPLPLFSRDTTASIVVGGYEIPAGTPVLIAPLGTHHRPELW
                     PDSQRFLPDRFLPEQESQRHRYAFIPFSAGPRVCIGNHFAMMEAQLVLTTLLRNYEFS
                     LPEGTVVVPEIDSALRPKEGLHMRIKRRTPRPAN"
     gene            61229..62704
                     /locus_tag="PKL17_00265"
     CDS             61229..62704
                     /locus_tag="PKL17_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNK43183.1"
                     /translation="MIVRNETVDPSTLSASETESLISSLYAVHSEIFDGVSRDQFAKY
                     VVCSPAKRTRIQLSYGHDGELAGYLAMHAFRPLPSGKECTVLRAEAGLRRAYRGNSST
                     AGFAISNLLKTKWDFGGPLYYLGCLVHPSSYTIFANNAPAVWPAPGVDIPPELFDLMM
                     RLGEEFHLEQVDPQRPLVRDVGWITRDTEVERRYWQTCDLPGARFYVEQNPDFTKGHG
                     LLTLLPLDLASTAKMVMNTGTSRLRKEIRRTVGSLERNVLRRTLDAPTAEELLQTVEE
                     VTGLDLDSVRNVGVLGTRYPIAARQTLFREGEPSDAMYVVLEGSLLVLGDSPEGELVI
                     DQLGANTLVGELEMMTGQPRATTVRAAIDTQLLRLSKTDLDKLLAAEPRLGSALWEHI
                     HGRVFALLCRRIPRLAQLSRHTQEAWFSAASSHVLTEPQQFTVLSPALLVLSKGKLVV
                     DRAGELMSVAAPALIPVAEGSAVQATGEAYFALLPPQPPSA"
     gene            complement(62701..63999)
                     /locus_tag="PKL17_00270"
     CDS             complement(62701..63999)
                     /locus_tag="PKL17_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43184.1"
                     /translation="MRLSTFASALLLFSSLVLSACAVDREVSDDPAANGVKPGPGPGP
                     GPGPIPNPSTGKLTGKVVAPEGTIPISGALVYVVPYAPTAIPDAVYCDKCVHIPDGTP
                     HTTTKPDGTFELDASTGNYYLVVQKGAFRRVRQISIVEGAQQIPKDKTTLPAITDKMN
                     GDDIPKIAVVVGAWDPIEVVLARMGLKATISKDLLGKAQVLAKDAPAFAIYGLQGLGL
                     PSPYPSVNTLLTDPKELGKYHILFLPCSGGTNQMDTGPKCTGVFNTDARVKSNLADFV
                     AKGGRIYVSDWSYEYVRQVFPGYITWRGESTQIGSACSGGGGNQSVYKKESGLDAWLQ
                     AQGKSLSEVKDAWTAIASVQDKMDIDADGKMSKITPKVWVETTSPVTASVQHGCGRVL
                     YTTYHTQPTSETSGALEPQALALLYLILEVGVCIDPLVIG"
     gene            64080..64766
                     /locus_tag="PKL17_00275"
     CDS             64080..64766
                     /locus_tag="PKL17_00275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK43185.1"
                     /translation="MASNNRAEQAATATLLLENVPLRGSFASLVPLLSQRKVCDLGCN
                     QGHYLQFAGDGSVGLDVSAESVLACSQKGLSARRHDFNSLPLPLPDASFEVVLLSHVL
                     EHVTMPLHLLRECNRIVQPGGTLIVGLPIEDGLYSRLRMDYFGGGEGHLFSFSLRNLR
                     KLMTLTGFAEQRVICHLPRLGNRLSAWNERLVRIFGDRLYTLSAAYWFVAQKIASPVA
                     DTELSDYFSR"
     gene            64774..65919
                     /locus_tag="PKL17_00280"
     CDS             64774..65919
                     /locus_tag="PKL17_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43186.1"
                     /translation="MNRLRPFAFALGFLLLVELLLRCVFAADERVLLDARNPFGCFAD
                     DPLYRLQKARELPEPALDVVLLGDSVLASVENGPGERLTDFLPGEVGRALADSLHGKK
                     VRAWSISNSAARAADQLAALKILHARLTKTPKGLRDLVVVISSNPLFFSRRFASDAMN
                     YPCLSYALADDPLLLRKLNIPSPSKGLWGDSDAYLGTLFSHLYLFQQRRNVAEKWVGG
                     PLRPRLRELVTHGVRRLSGQKESQVVVDEKTRNRPWFQRDHKRDSFSAHYDFLKSDSP
                     AAWNWTATRALFSYLHSHPELATLVMINPHNHFFIGPLGDTDGYRTLIAQLSQEASRQ
                     GVFLADYDRNPALQSEHFLDIDHFTRDGNLVLSSLIAGDVAKRVAGN"
     gene            65923..67140
                     /locus_tag="PKL17_00285"
     CDS             65923..67140
                     /locus_tag="PKL17_00285"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNK43187.1"
                     /translation="MSLLSLTYGVLVLCTVPLYALCPARLRTLLLCILSLLFYAWGSL
                     SHAALIVGLGTVVFFVGEQLHKKRSKLPYVLCVSLCVVVLCYFKYSILMLRTGADVLP
                     SGSLSALLSAHADSLALSLQIPLGVSFFTFEFVHYLTDVYKGKISHPEPLPVLRKRIS
                     DFFLFALFFPTLLAGPIKRYQQFHNDSQPAQRDMLAQGLWRILCGLLKKVLIADSLAT
                     ITLRLKAPELVTPTGLWLAMYAYAGQIYADFSGYSDVAIGVSLLLGYRIPENFNYPYL
                     AKSLQDFWRRWHISLSSWIRDYLFIPLGGSHVPPWRAFVNLILVMALCGLWHGPAGHF
                     VVWGIWHGAGLGIERMVRRLRGDSAEKESRGLPFLGWLWTFHFVCVGWILFAAPSLRS
                     AYVALGRMFFLLR"
     gene            complement(67111..67662)
                     /gene="ybeY"
                     /locus_tag="PKL17_00290"
     CDS             complement(67111..67662)
                     /gene="ybeY"
                     /locus_tag="PKL17_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNK43188.1"
                     /translation="MLSLQVRNSVRSLVPPPVRRELVRQLRRAMTAAGVDCTMVSLTL
                     SDNDELHDLNRTYADEDHPTDVLSFAQRDAEPAYHPPRSPDLPAEAFALPETLGDIVI
                     SVEIAQKQANEQRHDLLHELVHLSVHGLCHLLGMDHRDATEERWMFGYEALLRAQATP
                     KGRIVPQPMPERPVLAQQKEHSA"
     gene            67991..>68394
                     /locus_tag="PKL17_00295"
     CDS             67991..>68394
                     /locus_tag="PKL17_00295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43189.1"
                     /translation="MRTTRVGRFLLLREIGAGGMGSVFAAYDEQLDRRVALKLLRKPR
                     AGSRRQRMMVLREARAAARISHPNVIAIYDVGEIDGQIHIAMEYVDGETLLKWQKESQ
                     RSWRDILHMYLEVGQALQAAHQSEVIHRDFKP"
BASE COUNT        13562 a        19906 c        20833 g        14093 t
ORIGIN      
        1 ctgcaagcca acctgggatg cgtaagcatt ctgcgctaat cgcaccggac atcacagcgc
       61 gcgatagaaa aacgtggtgc tgcacagacc gccttgcgga aacagcgcat agtttggaat
      121 gtcgccgaca cgctgccagc cgagccgact gtaaagcctc gccgcgtcgc tgtccgtcac
      181 cgtatccagc acgaggagcg tcttgtttcg cttgcgcgct tcttcttcta tcgcgcggac
      241 caacgcttcc cctacgccac ggcgacgggc catgcggtgg acctgcatct tggcaacatc
      301 ggcgcgatgc ggctggttgt ctggcatgtg aagaacaagc tgcaccgtcc cgatgatttg
      361 ctgcgtcgcc aggtcttccg cgataagcac cagccgttca gcccgcgcga cagcagacag
      421 gaccgcttgc caaaacgaaa gagctcgctc gtaagacaga ggctgcataa aaccgatcga
      481 agcaccgccg gccacgcagt cgagcgtgac ttcggcgagt cccgatagga tcgctggatc
      541 ttgttcggca gagcgaatgc gaaaagagag ttcctttgtc accgtattcc tcgtgcgggg
      601 ttctatgtac catgccgccc atcatgcaat cggtccggtt tgggatgggc aacacgctgg
      661 cgttgggaat cctgctcggc ggaacggtgg cgtcggcgcg cgagtgccct gcgcccaagg
      721 gatcgagccc cgaactggcg tctgtgttgg ccgaagatcg aatccgtttc cttgcgcgga
      781 cgctgaccga cacggcgcgg cgcgagcgtg actatgcgat tggctggagc ctgacctatg
      841 cgggactttc cgcggcctcg tgggtgatgt tgccgctgtc gtcgtccgac gatccggggc
      901 agcttgtcga aacggcatgg aactcgggca cgtccgcgtt tgccgcgctg tatgttctgt
      961 atgcgccgct gtccgcggtg cgcgctgtgc acaaattgaa ccagcggctg gcgtccgcaa
     1021 atgacgatgt ctgtgcgcag cttgccgaag ccgagcgcgt gtttttcaaa gtcgcggaca
     1081 accaaaaagg cggtcgcgat gcgctggctc acatcggcaa cgtggcattt aacgtcgggc
     1141 tcggcttggt gcttgggtat gcgctgcatc ggccaaacgg tgccgcgatc agcacgagca
     1201 ttggtgtcgt catgggcgaa ctgatgatcg ccacccgtcc gtccggcgcg ctgtctacgc
     1261 tggctgacta tcaaaaaggc cgcttgcttg ctcccccaac atcgccgtcg ctggctttgc
     1321 cattccagct tgtgccgacg ctgcaaaacc agacatacgg acttgcgctg atcggatcgt
     1381 tttgaaacgg ggattaggaa tcgcggtgga cggtggcggg tgaaaaggcg ggcagacaga
     1441 ccgcgatgta ctcggcgcca ttgggacccg gcgtgctgta acgaacccac tcgcctttgg
     1501 gagccaggat cgcttcgccc gcgtgaacgt cgatgctgcc ttccttggtc gtcacggtca
     1561 gcgtcccggc gagaaccacg gtgtattcat caaactcggg agtctgtccg ggttcgaccc
     1621 agccgctcgg gctgcgcatc cgcgccatgc tgagcgcgtc ggtctgcgaa ttgacgcgac
     1681 caaagaactc ttcgatcagt ttgggcttgt tgccggcggc ttcgatgacg gtcggtttgc
     1741 ggatgtgttt caccatgacg aaacggtagc gtgtttcgtc gcggcggggc aaaaaaaccc
     1801 tgtgctgact agcgggcgga tatgagcgcg tcggcggcgc tttggcaggc ttttagaatc
     1861 ggcgctggat agacacccat tccgacgacg agtagcagac agacgacaat cgttacggcc
     1921 agccacggtt cgacttgcac cggttcgctg cgggtgggct ttgccatgta ggcggcgcgg
     1981 gcgacttgca gatagtaaaa aagaccgacc actgcgagca ccgcaccaac gacaaccatc
     2041 catcccagac cggcctgata ggtggccata aagacgtaca gcttggccca gaaaccgatc
     2101 acaaacggaa tgcctgccag cgacaaaagg aacatcagaa gcgaaagacc cagccacggc
     2161 gatcgctttg ccagaccggc aaatccgtcg agcgtgtcgt ctccttcctg gtcggccact
     2221 gcatgcacca ccagaaaggc tccgatgttg gtcacggcat agccgcacag ataaaacagg
     2281 agcatcccca gcgcatacgc atttccgacg cccagcgcga gcagcatgta gccaatctgg
     2341 gcgacgccgg aataaccgag gagccgcttc acattctttt gcggcagcgc catcaagttg
     2401 cccaccgtca ttgtcagcag cgacagaccc aagatcgccg gctgccacag cgcttgcggc
     2461 gccgcttttt gtgacagcac cgtcagcgtg ataaggcctg ccgctttggg ggccaccgat
     2521 aggaacgtca ccatcggggt catcgcacct tggtaggtat cgggaaccca catgtgaaac
     2581 ggcaccgcgc caatcttgaa acccataccg ccgagcacga gcagcattcc catcatcgac
     2641 agcgcactgg gtggggccgc cagcagggct tcaagctgcg tgcttccagc ggagccaatg
     2701 agaagtgaca gaccaaacat cgtcaccgtc gttgatgccc cgccgaccag atacagcttc
     2761 agaccggctt ctgccccgcg gcgcagcacc gcttggcgtt tttccggggt cgattcttcg
     2821 cgtgaaagcg ccgcgagaat gtagagcgga atgcccatca gctcgaagct gacgacgagc
     2881 aagatcaggt cacgcacgcc cggcagcagc atcattccac aaagacacat gagaaccagg
     2941 aagtgatact cgccttgctt ctttgtgaag gtctgtgaca gccattcctt cccaccgagc
     3001 acgcagagca gcgtcgcaaa cagcaggatc tgtttgacca gcaccgaaaa cgcgcttccc
     3061 acgtagacgc tgccgatgct gcctgtgcca tcgagctggg tcgtcaggta gagcgcaacc
     3121 gccaaaaaca gcgctgtcag cgtcgacagg tgtttccgcg cggcgtccgg cagatacatg
     3181 tccacaagca gcagcagcaa ggccgaaccg gcgacaaaca gatcgaccca gatgggctgc
     3241 aaaagttgga agctcatgga agcaccccca ccgggagcac agagcccaag gccatgcgca
     3301 gatagttggt cgtcgtcgga tcaatgaagt cgagcagaag ccgcggccag attccgcaca
     3361 aaagcagcgt gccacccaag atccaaagcg gcgcccattc gatttttcgg acatcgcgca
     3421 gatcatgaaa gtgatcgctt ggaccgtcgc caaagaacac cgcacgcgac acccgcagca
     3481 catacaccgc ggtgacaaac gctccgatga cgccgcagat cgaccaccac ggacgcggac
     3541 tctgccagcc gcctgcgaac accaagaact cggcgacaaa acccgcggtg cctggcagtc
     3601 caagcgagga cagacccgcc agcgtgaaga acgcagcgac gcccggcatc accgtggcaa
     3661 agcctcccat tttggcgact tcgcggctgt gggctcgttc atagatgtgc ccgaccagtg
     3721 cgaagaacag gccggtcatg atgccgtgcg cgaacatttg gtagaccgcg ccgttccagc
     3781 ccgactgggt cagcgtcgcc gctccgagca tcaccacgcc catgtgactg accgacgagt
     3841 aggcgatgat gtacttgagg tctttttgcg acagcgctga catcgcgcca tacacgacgt
     3901 ttacgaccgc aacaccaccg acgatggggg cccagaactg ggctccgtcc ggcagcatca
     3961 tcatgccaat ccgcatcacg ccaaacgcgc ccagcttcat cagcacaccc gcgtggagca
     4021 tcgacactgc cgtcggtgca gatgcgtgac catcgggcga ccaagtgtga aatggaaaca
     4081 cgccagcgag cgatccaaag ccgagccaga acatcacgaa cgtccagatt tgcaggctct
     4141 ttggatagtg cgctttggcc agcaccgcca tgtcgaacgt ccccgctccc gacgaaaagt
     4201 acagcgccag gagtccgacc aggataaacg ccgaaccgac cagcagcatc agcgtcagct
     4261 tcatcgcggc gtattcgcgc ccgccgatgt caaaccactt ccagacaaaa cgcagcgggc
     4321 cggcctcggt gactttgtgg ctcgatcccc agatgccgat gagcagatac atcggcagca
     4381 ccgcgatttc gtaaaacagg aagaacacaa acaggtcttg cgcgacgaac acgccgaaga
     4441 cgccagcgac cagcacgagc agcagcacga agaactcttt gggccggtcg gcaagtccga
     4501 agctcgcaag cacaccggca aagatgatga cgccggtcaa aatcagcagc gagacacccc
     4561 aaccgtcgac ggccagccgc agatcaatgc caagctgcgg caccagtgga tagacttcgg
     4621 aaagctggaa accgctcgct tgcggattgt aaagctgcgc gacatacaga ctgccgagca
     4681 gcgacaaaaa cgcgccaagt gccgacacaa gacggatcag cgtgtgctgc ttgggcggca
     4741 gcatcatgac aacaagcgca gcgaccatcg gggccgccac aatcaaggaa aggatgtgct
     4801 cggtcatcgc agcaccccgt aaaggacaag cagaagcacc ccgcacaccg tcgccagcac
     4861 atagtctgtc gcgatgccgg tctggactct gcggattttt tggccggaca ccacaaaggc
     4921 aagcccggtc aggttcatga tgccgtcgat cagatagcga tcgatccagc caatcagcgc
     4981 tgcaaacggc agtgcgacgc ggtggaatcc ccctgcatac agccggtcca tgggtccgta
     5041 ttcgacaagc cgtgcgatgg gggcgagcag ggcagggtgt tcatcgctgc ggcggtacag
     5101 cagatacgca agcccaagtc ccaaaagacc cagcccagcc gcgatgattc ccgtcgtgcc
     5161 caagtggaag tggtacgcgc tgccgtatag ctgtccaaac atcttgccca agtacccagc
     5221 agcgagcgcc ggcaccgcca gcagcagaag cggcagaatc agcaccgcgc tgctttcatg
     5281 ggcgtggctc gctttttccg tcgcttgcgc aaagaacacg cgcacaagca gccgccccat
     5341 gtaaaacgcg gtcaaaaacg ccgagatcag cagaaacaca agctgccagg acacgccatg
     5401 ctcaccaacg gcttcgatga tcaggtcttt gctaaaaaac cctgacagac ccggcagccc
     5461 catcagcgac agcgatccaa tcaggaacgt caccgtcgtc agcggcatct tgcgggcgag
     5521 tcctcccatg tcacgcatgt catttgagtg aaccgcgtga atcacactgc cggcgccaag
     5581 gaacagcagc gctttgaaaa acgcatgcgt cgtcaggtgg aaaaatccac cgagcaaaga
     5641 cccggctcca agcgccgcaa tcatgtagcc gagctgggaa caggtcgaat acgcgagcac
     5701 ttttttgatg tcgttttgga atagcgccat cgtcgctgcg ataagtgccg tcacgcttcc
     5761 caccgccagc atgatctgtc gcgttccctg cgccagttca aaaagtggcc agccgcgcac
     5821 gatcagaaac acgcctgctg cgaccattgt ggctgcatgc agcagcgccg acaccggcgt
     5881 cggaccttcc atggcatcgg gaagccagac atgcagcgga aactgcgctg actttcccat
     5941 caccgcgaca aacaaaagca gcgtcaccag gttggccgcc gacggtgcaa tctgcacatc
     6001 ccagtcaaag ctgccggcct tgctgcgcaa caccaaaaga ccgatgagga gtcccatgtc
     6061 ggcaaacttg gtgacccaga aggctttgac tgcggctttg gcggcgctcg gttttttgaa
     6121 gtagtaaccg atcagcaggt aactggtcac accgacgagc tcccagccca aaaagagctg
     6181 caaaagattc ggtgccagca ccaggatgtt catcgcggtc agaaacagcg agtgatacgt
     6241 gaagtagcgt ccctgcgctt ccttggattc atcgtgcatg tagccgatcg aaaaaatctg
     6301 cacgcatgac gccacgaacg tcaccaccag cagcatcgac gcggaaattc cgtcaatgcg
     6361 cagaccaatt tcggcgagcg gtgctcctgc ttggggcagc cacggcaccg tccaaagctg
     6421 cggcggcgtc ggcgtaagct gtcccaagac gagcagaatc gccgatccaa gcgaagtcag
     6481 cgccgccagc accgaaaaca caatcgccgt cacgcggtgc cgcagggctg gtactgttgc
     6541 cagcagcagc gccaccgctc cgggcaagag cagcgcgatg agcgcgagcg agtaagcaga
     6601 gtgaaacatc gcgggtgtct cctactcttt gaattcgctc gcccggctta cttgcgcgtc
     6661 gcggtaagcc cggtgaacaa ggatgacaat cgcgagtccg accaccactt cggtgacggt
     6721 gagcgcgatc gaaaacagcg caaacacctg gccgattccg tcgccgcgga agcgcgaaaa
     6781 cgataccagg ttgatgttgg tcgcattggc cataagctcc aaagacagaa ggagtccgat
     6841 ggcgtggcgg tgcgtaagca gtccgtaaag accgatgcag aacagcgccg ccgacagggt
     6901 gaggcacaga ccgagtgtca tcgtccatcc tccgaaatct gcgcgggacg atcacggcgg
     6961 agcagcacaa tcgcgccaat catcgtcgcg agcagcagaa gggacagcgc ttcaaacgaa
     7021 agcgcatgat cgacaagcag cagtttcgca agctgctggg tcgacgcagg cggcgcgtcg
     7081 cgggctggca gatccggagt cgactgaatc gctttcacca gcatccccag catcgccagc
     7141 gcgagcactg cggcaacata cggtttcttg tgcggcagcg cggcttcctt ctgatcgtgg
     7201 cgcgtcagca tcacaccaag gatcatcagc gtcatcacgc caccgacgta cagaagcact
     7261 tgaatgcccg cgagcagcgg cgctttgagc atggcgtaca gcaccgcggt tgcaccaagc
     7321 accacaccaa gccacagcac gccgtgaatt gcgctttttg cccgcgcaac caaaagggcc
     7381 gccagcacaa tgcagccagc gacgaggcca aagccgactt gctcgatcat ggggcctcct
     7441 ttgggtcggt tttttgttct gcggtgtccg ttttttgttc tgcctgcgca ctcaccgggg
     7501 aagccggagc ggtctgtttg gccgcggctt ccgcagccac cgcaggcttt gcgtccgtcg
     7561 ctggtgccgg ttccttggca gaatccgtgg tcgcgaccgc ggtttctttt gctgccgcag
     7621 gtgccgcttc tttggcgggt tcggcagcgg ctttggcagc ttttgctttg gcgactctct
     7681 tgggtttttc tgcgccagct tccgccgatt tttcgtcccc cgcggcttcc gctggcttgg
     7741 ctttgccgcg cgccggatct tgcatttcca tcagcttgcc gccggttccc cacgacagcg
     7801 gcttttgctt ctggttttcg tacagcttgt ccatcgtaag gaccaagtct tcccgacagt
     7861 atccgggctg ctcgggcgca cgacacatca caatcgcgtc ggtgggacag acctggacgc
     7921 acagctcgca gaagatgcag gcttccaaat cgagcgtgaa gtcttccgca tagccgcgct
     7981 ttttgccggt gactttcgat tcccgcttgg gtgcctgttt gatggcaatc acttgcgaag
     8041 gacagatccg ctcgcaggcc agacagccga tgcacgcttc gtcgccggtt tcatcaatca
     8101 gcagcgcaaa gcttacgcga tggcgttctg gacggggccg gatgacttcc ggatattcga
     8161 cggtgaccgc cggtcttagg aagtttgtca gcgtggtttt gagtgcgcgg aaggaatcgc
     8221 caaaaaatcc catcgctagc ctcccacctt cgcaaacgcc gctgctgcca tcacaagcag
     8281 cagactcacc ggcacaagcc acttccagca cagcgtcatc agttgatcgg accggaagcg
     8341 caggagcgac caccgcaccc aataaatcga cgcaaacagc agcagcgtct ttagcaccat
     8401 ccagtgcagc cccggaaaca ccggtccatc ccagccaccc aggaacagcg ccgacgccac
     8461 cgccgacgcc accagcgtgt ggatgtattc cgccatgtaa aacagaccga acttcatgcc
     8521 ggtgtactcg gtggtgatgc cggcgatcag ctcgctttct gcttccggaa tgtcgaaagg
     8581 aatccggttg gcttcggcca gcgatgacag aaaaaacagc acaaacgccg gcagaccggg
     8641 cagcgtcggc cagcacgcca cccagtgatg atcgatttgg tagcgcacga tgtcggaaag
     8701 actcatcgag ccggccaaaa tcaccggcac cagcgccgac agcaccatcg gcaccgaata
     8761 ggaaaccgac tgcgcgaccg ctcgcattgc accaatcagc gcaaacttgt tgttcgacgc
     8821 ccagccgccc atccacaccg gaatcagcac cagcgcattg gcggccaaca cgtacagcac
     8881 accgatgtcc agatccgcgg ccaccaccga cggtgaaaat ggcacgactg ccgcagttgc
     8941 cacggcaaac gcaccacaca gaatcggcgc cagttcaaac agcggacgat cggcggcggc
     9001 tggcgtgacg tgctcttttt gcagcatctt caccacgtcg gccagcggct gcaggattcc
     9061 tgccggtccc accatcgtcg gtcccatgcg actctggatg cgcgcggcaa acttgcgttc
     9121 aaaccacaga ccaatcgcag tcaccgtcag cacaacagtg atgatcggca gaccatagag
     9181 aatgccgtga agaagtgcgt tcacctgtcc acctctccca tgatggggtc cagcgagccg
     9241 aggatgacga cagcatcggc gacgacgtga ccgggcaaaa gatagggcag cgccgacagc
     9301 gcatggaagc tgggcgggcg aatctttaag cggtacggac tcgcattccc atcgccacca
     9361 ccggcaatca cataggtgcc cagctcaccg cgggatgatt ccatcgccgc gtaaacctga
     9421 ccctgtgcga ctttgacctg tccgacgagc ttgatcggtt tgtagccaca gatcggacct
     9481 tcaggaaccg catcgatcat ggcgcggaca atccggatgc tttcacgtag ctccgcaagt
     9541 cgcaccaccg atcgagccca gcagtcgcct tcctttgccg tcgctacgtt gacttcgatt
     9601 tcatcgtaag cgtgatacgg ggcatcgcgg cgcagatcat gatcgacacc gctggcgcgc
     9661 agaatgggtc cgctaagacc cagttccagc gcaagttctc cgtcgagaac gccgattcct
     9721 ttggtccgtt cgctgaagat tttgttcgcg agcgtgatgc tttcgtattc cgaaagtcgc
     9781 gactcgatca cctgcagcgc ttttttcact ccgtcggcaa agcccggcgt aatgtcgtgc
     9841 cgattgccgc cgatggtgtg cgtgttgtag tggaagcgcg cgccggtgac ttcctcgaac
     9901 agatccagaa tcatctcgcg ttcgcggaag cagtgcagaa acaccgacgc accaccaccg
     9961 attgcaccac cgagatcgag cgcaaacgta cccagccaca gaagatgtga cgcgatgcgc
    10021 tgaagctctg caaaaatggt ccgcagatag cgtgcccgtc gcggaacttc gacgccgagc
    10081 agcttttcga acgccatgtt gatcgcctgc tcgttgtgca tcggcgatac gtagtcgttc
    10141 ttgtcgacga ttggcgtgac ctgcacatag gtcagtcgct cgcacagctt ttcgacgccg
    10201 cgatgcagat agccaagatc cggaaccgct ttgatgatct tttcgccatc cagataaagc
    10261 tgcatccgga acaccccgtg cgtcgacggg tgctgcggtc cgaagttcag caccatcagg
    10321 tctgcttcgg gttcatacag atccagcttg aggtgtggat tttgccgttt gatttgcaca
    10381 agactcatcg gcttacctcc acggggcaca cggtgcgtcg gcggcgtgat cacggcgcag
    10441 tgggaaaccg gaatagtcgt gcggcaaaag gatgcggcgc aaatccggat gtccggcaaa
    10501 gcgcacaccg acgagatcga actgctcgcg ttcttgccaa tcggccccgg caaagacgcc
    10561 atacagcgag tcaattccgt cgggtgattc aaagcggacc cgccacaacg caagctgcga
    10621 gccttttccc gtcgtgcgca gcccatacat cacttcgtac tgctcgggct ttttgacgtt
    10681 cccttcgctg tctttttcac ccggataatg cgacgccacg acggtcacaa gctgacggaa
    10741 gccttttttc ttgagcgtct gggccaagtc gcgatgtctg tccacgggaa cgagcggtgg
    10801 cgcatctttg ggaaattcgc tgacgccgtg cgcacgcaac atggcttcga gatcgccatc
    10861 tggtgacact tgggtgtcac ttgtcgtcgc aggtcgcggc gcagtgggcg cttcgagcag
    10921 cggatcagaa cccgcgactt tcatcgcgct ctggagctgc gcttcatcgg ttttgggatc
    10981 acgcgccgga tcgccgagcc gacgcagcgc cggcatgatc atcttggccg tctcaggccg
    11041 cgtttccttc tgcgtccact tgcccgcgcg ctgcagtcgc actttttcct gaagccgcag
    11101 cattcctgcc atcagcgctt cgggattggg cggacagcct ggcacataaa catcgaccgg
    11161 caggaacgta tctattcccg gaaccgtcgg atacaggtct cgataaaagt cccccgagat
    11221 ggcacaggaa cccatcgcga tgcaccactt gggttccggc atcagatccc acagcttttt
    11281 cacccgtggc gccatcttca cggtgatcgt gccggcgacg accatcacat cggcttgtcg
    11341 tggcgtgccg cgcacgatgg tgcccatgcg gtcgatgtcg acgcggcttg cggcggttgc
    11401 catcagctcg atgccgcagc agctcgtcgc cattgggaac acccacagcg aattggtcag
    11461 tccccaggta agcagcttgt cgacttggcc cgtcgccact gacagaccgg gcacccccat
    11521 catgaagtca tagagcgggt gcgaaaacgg caggttgtcc tctattgcca tcgcagcgct
    11581 ccttttttca ccgcgtagcc ccagccgagc agcaggatgc cgacgaaaat tcccatctcg
    11641 acgacaccaa ggtcgcggac ggacacgccc cacggcagca tgaacgcagc ttccacatcg
    11701 aacaggacga agaacagcgc cagcacgtag tagcgaggat gaaattgaat ccaggtcgga
    11761 cccgcttgat cttcaccgca ctcatacgtc tgaaacttga gcggcgattc cttgcgtgca
    11821 cggacgcgga gaatccagcc cgcgcccgcc atgctaagcg ggaccacgaa tgagagggcg
    11881 agaaagacca gcagagcgag ccatgcgtct gacatctctt ggcacctcac ggaccgcagc
    11941 ggcagttgag cacgccttcg cccaactggg agtcattccc actgtggctc ggacgtgtac
    12001 ctgacagttt gtggccagac cttacgagtg ttcgagggtg ctgtcaagct gacagcggtg
    12061 ggagctctcg tcgggaaaaa tgaatgccct ggcgcgttag gccagggtat tgagacggta
    12121 cagccttggc acgggaacag agaaaaagag aactgtgggg ggagtaataa gtgcgagggg
    12181 ggactacggc gtgcttggtc gaatcaggat gtagacgctg gagtcgccgc gctggatgag
    12241 aagcacatga ccgccggggc gcgcttggcc aagcagcttg gtcgcttcgt ccaccgactt
    12301 gaccggatgg cgatcgactt cggcgatcac gtcaccttgg tgtagaccgg cctcggcggc
    12361 tgggctgcct ggcttgacgc cgccaatcac cacgccttga ccgcctttga tgcccagtcg
    12421 ttccgcgagc tgcggggtca gcggttgcag tgccagaccc agtttggccc gcgctgagtt
    12481 tgcatcgtcc gtcgaaccag cgctttgagc cggtgcctca tccgagggga gttcgcccgc
    12541 cttggcgctg attgtgacca ttttgccgtc gcgccagatt tccagtgcaa ccggatcacc
    12601 gacgcgatgg gtcagcactt ggcgctgaac ctcgcggctg ttcgcaatcg ctttgccgtc
    12661 gactttggtg atgatgtcgc cgcgtcgcac gccggctttt tccgccggac ctccgccggt
    12721 tagaccctgg acgagcgcgc ctttttccgg tccgcccatc gctttggcaa ggtccggttc
    12781 gtaaggctgc atcgtgatgc caaggaacgg acggtgaacc ttgccgctcg cgataagttc
    12841 gctgacgaca ggcttggcca tgtccgacgg gactgcgaag ccgatgccga tgttgccgcc
    12901 gggccccagg atggcggtgt tgatgccaat cacttcgccc gacagattga cgagcggtcc
    12961 acccgagttg cctgggttta tcgaagcgtc ggtctggagg aagtcttggt agttgctgcg
    13021 gttttcgcca atgcccgagc ggcctttggc gctgatgaca ccgacggtga ctgtgtggtc
    13081 cagaccgaac gggttgccaa tcgccatgac ccattcaccg acgagcagtt tttcggaatc
    13141 accaagccgc gccgccttga ggctgtagtc tttcggatcg acgcgaatca ctgcgaggtc
    13201 agagcgggag tccgtaccga cgatcttggc ggggagctct ttgccgtcga tgaactggac
    13261 tttgatgtca tcggcttcac cgacgacgtg gttgttggtc aggatgtagc ctttggcgtc
    13321 gatgaccaca cccgagccgg caccgcgctg gaccgggcct tggcgttctt cggtgccatc
    13381 atcctcgcca tcgtcatcag gcattccgaa ggggctgcca aagggaccaa agtggaacgg
    13441 cagctcgcgg ccaaatggac tgccgcggcg catgactttg atcttttgct tcttggcgac
    13501 gcggatggcg acgacgctgg gggatacttg ctcggcgact tgcgaaaacg tgcgagacaa
    13561 gctgcgtgcg tcggcgatct ggcttggggt gggcgggggc aggctcggcg cttgggtttg
    13621 cggcaacagc ggggcgacgg cagccggggc ggcggcacca ggcgcggcgg cagatgcttg
    13681 ctgatcccag ctcactttga ccttggtgca ggcgacggtg ataagtgccc cggtggtaaa
    13741 gagggcgagg ctgcggaaca agcggaagga agtggacttg ccagtcatat cgctactgtc
    13801 ctccaagagg tggggcgtcc ccaaatcgga attgggacac atcgaccgaa aagctagtta
    13861 ctgcctgtgg aacattcaag ggggcgcggc tattcctagc gggcggaaat tttgccaaaa
    13921 accgccgcac gggctgtggg caggctcttg tctgatcggc ccaccggggc tagctgcgac
    13981 ggaattgcac cggcggccag gcgtccccga gtgccgactc gacatcctcg ggacgcaggc
    14041 gctcacgatt gctgtaaagc atgatcgccc gctccagcac cgcttccagt tcgcgaaagt
    14101 tgccaggcca gtcgtgccgc ttgagtagcg agagcgtgtc ttcttcaacg agaaacacgc
    14161 tatcgggcga agctgggctg ttcgcatcga ccagctcggc ggggcgttcg tcgtggaagc
    14221 tgcgcaggat gcggttgcag atccacagcg tcgagtgcgc gatgtcttgc ggcctttcgc
    14281 gcagcggtgg aacgtgcagc acaatccgca ggagccgata ggccaagtcg tgccgaaact
    14341 gcccagcccg cactgcctgt ggcaagtttc cgtcggtcag cgcaatcagc gcgacgccgt
    14401 tttccgtccc cagccggtgc tcttgcaagt gttcatgcag gctgcgctga acatcgagcg
    14461 gcatcaccag cagttcgtcg agcaaaagcg atcctccatc ggctttcgac aaaagaccgt
    14521 cttcatagcg actgcatact gctccgtcgc cgctcatgta ggtgtcgggg ccaaaaaact
    14581 gctttagcat ttcctccgac gaacagccgg ccagatgcgc acgaacttgg ggcgcgccgc
    14641 tcggatgcat cagtcgatgg aggagcttcg ccagaccttc ttttcccgtc cccggctcgc
    14701 cataaatcac gacatgggtt cgaaacggac ggtcttgcaa ccgctcgatg actcgcagaa
    14761 tgcgcgccat gtcggggtgt gcgtcggaca tgcgtccaaa cgccgaaatc caggtactca
    14821 gttgcagctc tccgccgcca aagatcccgg acgtttcgtt cggtatggcg ctgtgggatg
    14881 aagcggtctc gcgcgctttc ctcataaaca gacacgatat cagagtggcg cggctcgaaa
    14941 aaagcgactc ttgacgctat gccgcaagcg gactgagact gcgaagacgg tgcaaaagcg
    15001 atttttttca agtcgagcct gtaagacgac gctgcttggc tgggttctgg gcccgatttt
    15061 ttcgctgtgc ggcgtgacgg ctgccgccga gacttcacct gtggggccgg tgccgcttac
    15121 gccacctgca ctgccgacgg agcctttgcg ctacagcgac gaatgggtga aaaaagaact
    15181 gaccgagctg cacctgcgct atccgaagct gaccaagctg gtgccgattg ggaaatcgca
    15241 cgaggggcga ccgctgtggg cactggcgat tggtcgcaac ctgaagcgcc acgacggacg
    15301 tccggccatt ttggtgaacg ctgcgcacca cggcgtcgag acgttttcga tagacctggc
    15361 gctcgatgtt gccgacgtgc tgcttttgcg cgcgggcgat gaccagcgac aggggctgcg
    15421 gatcgatccg ggcctggaca aaaaagtgca gcgctggcta aaagaactgg tcatctggtg
    15481 cgtgcccgtc gtcaatcccg acggtgtttg ggcatcgctg catggttttg tgcgcaccgg
    15541 acgcaaaaac ggtcgcgaca acaacggcaa cggcaagccg gatcgcggcg acggcgtcga
    15601 tctcaaccgc aactatccat ttcgctgggg ctttcttggc gacaaaggca gctcgccgca
    15661 gcccgacagt gcatattttc gcgggcccgc tgcgggatca gagccggaaa cccaagcgat
    15721 tatgcgcctg gccgaaagtg agcactttgc agcttcgctg tcttttcata ccggcaatgt
    15781 gacggtgctc gcgccgtata ccatcgacaa cgtgcagagt ccgacgccga acgaagcgtg
    15841 gaccgtcgct gaagagctgg tcgccgggct gcctcgtcat ccgcaaggca aagacatcat
    15901 cgtccgtcgc aacatctatc ccgtcgatgg caccgatcag gactatctgc gcggacagtt
    15961 tggtacgctg gcgctgctcg tcgaaggtgc gcggcgtgat ccgacgcagg ccgaggcgcg
    16021 acgcaacgtc gtcttggcga tgcgaccgat ctggacgcga cttctcgatc ggtatttgga
    16081 tggtccgtcg gtgtatggtg tggttcggga tggcaaaggg cgtccggtcg tcgcggacgt
    16141 cagcattgtt gagcagacga tggccgaaca agagcactgg cagacccgct gccgcgacgg
    16201 acactacgat cgatttttgc cagggccagg ggaatatacg gtgcgggtga cggcacctgg
    16261 ggtcgcgccg aaagatcagc ggatcaaagt cggcaaagaa cggctgcgga tcgattttac
    16321 cgtcgaggct gcggcttctt ctggacagtg cccgaaggaa ccaccgggat aggctcgggc
    16381 agcggtgggg caccggcgcc cggtttgacc gccagatggg ccagctcggg acgcagcacc
    16441 gctgaaaacg caccgacgat gcgtccccac agtcgctcgg tgaccactgg atcgggaacc
    16501 atgtgggtga gccccggcag cggcagaagt tcgtgcggct tgccggcgcg gaacagtgct
    16561 tgggacagct tcaggctgtg gaaaaacagc acgttgtcgt ctgcggttcc gtggataagc
    16621 acaagcggcc tgcgaagctg cgacgcatag gtcaaaagcg acgatcgctc atagccgtcg
    16681 gggttttcct gcggcgtgct gatgtagcgt tcggtgtaac aagtgtcgta gtcgcgccaa
    16741 tcgacgacgg gggctccggc gacaccgatg tggaagacat ccggcctgcg caaagccgcg
    16801 agcgccgcca tatagccgcc aaacgaccaa ccgaaaatac cgacgcgcga gagatccatc
    16861 tctggatact tgtcgcctgc ggcttgcagc gctgcgacct ggtcttcgag aggaatgtcg
    16921 gcaaaattgc ccgcgatcgc gtgttcccac gtcgtgccgc gtcccggcgt cccgcgtccg
    16981 tcggccataa acacgatgaa gccatgatcg gccagccact ggtcgagcat gtacattccc
    17041 gcaatcgcca tcacttggtt gtggtgcggc ccgccgtaga catgcaccag caccgggtat
    17101 tttttccccg gctggaagtt gcgcggtctg acgagcagcg agcgatagtt ctgcgtgccg
    17161 acctgcgtca cttccaggtt tgggacaaac ggcggcgtcg tggacaccga cggaagctca
    17221 ccaacaactt gatcgcttgc tgcggcttgg ctggattcgg gaacccgcat caccaccgtc
    17281 ttgccaagct gcgtcgtcga aaacgagcta agcacataga cgccgggatg ttgtccgaag
    17341 ctggccgcgt gatagccgcc cgcttgcgtt agtcggagcg gcacacccga cagcatcgac
    17401 acgcgataga tgtcttgctg cgtcggctcg ttgctggcgg cgtagcttat gtaaccgctt
    17461 tgctcatcga cgtggagcag cttgcggaag ccctggcttt tgtcgacgac caggcgcttt
    17521 agtccacctt ggtaatcgcg cagctcaagc tctctgctgc cgtctcgttc actggtccac
    17581 agaaaacccg aaccgtcgcg cagcggcctg gggacatctt gatcaatgtt gatccatgcg
    17641 tcactgtgtt cgtgcaaaag ccgctgggtc tgccccgttt ttagatcgac cgtgacaagc
    17701 tccagatcgc gctggctgcg gcttagcagg gtcaaaagca gcggtccgcc ttcgcgccag
    17761 accaccgtcg ccaagtaggg cagtttgtcg cgctgccact gcacccactg gggcgcggcc
    17821 cccgtcaggg cgataagtcc aagccgcact tcggcgttgt cggttccggc gcgtggatag
    17881 cgatgcggct gcggcggctg ctcgggatga agtgcatcac ctacgtacag cgtctcgacc
    17941 ggctggttgt ttacctcggt aaacgcgatc atcgtgctat cgggcgacca gaaaaaaccc
    18001 gaaaaacgat gcagctcttc ttgggcgaca aactcggcgc tgccatagct tatgaccggg
    18061 tttgtgctgt gcatgagccg tcgctcacgc cgcgttgcca aatccatcac gtacaggtca
    18121 cgcccgcgga cgtaggcgac ggctttgccg cttggcgaca gatgcggatc gacgatcggt
    18181 tcaccgctgc gctcgcttgg atcggtgacg ggggttatct cgctcgaatc ggcggtccgc
    18241 agctgatagg tgtagagctt gcccgacagc ggaatcagca gaaagctgcc gtctttggac
    18301 agctcgaacg acgtgaatcc gcgcatcgac tgtcgcattc gttcgcgtcg tgctttttct
    18361 tcgacggaaa gatgctcctc cgcaccttgc agaagctgcg ctggggacag cagctctcgg
    18421 gtttgtcccg tcgctacatc cagaacgtgg agccgcagct cgggcgaagc cgccgtggcg
    18481 cgcagaaaca gcacttgctt gccgtcgggc gtcggggttg gacgcatcgg cttgccgagt
    18541 gaaaagccgc gcgttctggc atgctggccc agataaccgg gaacaggacc gatggtttcc
    18601 gacggaccgg gcgaagcgtt ggctacagaa gagagggaca gatacaggag cagtgaggct
    18661 ctgcgcatag agaggaaact atcacaatca ctttgggcgc gccgttcgct tctggatggc
    18721 ttcgcagatt ggctcggggc gacgcaagcg gttcttcact tgttcctcgc tgacgttgta
    18781 cgcggtctgg aattcgaggc cgtcttcgta cagttcgtcc cacgatgctt cgtctgcgac
    18841 gtattgggcc ggacgtttgc ctttgtacac caagcgtccg aggtttgcgg cgcgggcgcg
    18901 aagaccgttc cacagctcgc tgccgagata gcccgaaaac agcgttagat cgtcgtcgag
    18961 cgtgccaaag atgtcgtcga agcgactgcg aaatcgatcg gcttcttcgc tcttgtccgc
    19021 tgcgatgttc ccgaaggcaa tgcgcagcaa aaagacgtct tcaaggaagc gttccggacc
    19081 ggacccgtct tggtgcccga acgtttgcac aaggacactg gcggcttgtt cgcggatgtt
    19141 ttggggcagc tcaacgtcgg tgatgacctc ttgcagcact ttcaggtcaa acggcaacga
    19201 caagagcaag tcgcagaccc gtgacaggaa cagttgccca ttctcggtgg ggcgcatgat
    19261 ttctcttacc tgataccaag ctagactttc aaggatagag acggaatgcc ggtctgtcta
    19321 cgacgggctg gacgagggcc ccgctttttc ttagcctaga ggaatcactt gggggagtcg
    19381 tggacgccat tgttttgcgc cgggaattgg cctgtgcgac ggcaccgagc gcgctttttg
    19441 ccgttcttgc cgacacggat aggctgtatc gcacgctcgg tcaagtggca gtaagccgag
    19501 agccgctttc tggggaaggt tcggcgcggt tccttttgcg agctcgtggc gaggcaaaag
    19561 cgatcccgtt taccgaaatc ccaccgcagt ggagtcatcc gtcgctgcta gtgacaaaac
    19621 gggtgctgca tcagggtttt ttggccagcc tggcgacgcg atttacgctt acgccgcgca
    19681 tgcagggcac gcagctcctc attgaaatgc aggtcgagcc gcggcttgtg cagcttggct
    19741 ggctggtgaa gctgttcgcg caggcgacgc tgtggcactt gagtcggacg atcctgcgga
    19801 tcgacgacgg aattccacgc ggcgagccga cgcagtttcg tccggcgcag cttgctgtcg
    19861 aaccgcttcg gcaagcgcag cagcaaacca aaacgcagct tccgcctgaa gaacagagcc
    19921 aggtcgacag tctggtcgca catcttttgc gcaccgacga tctggatgtc gattgcctgc
    19981 gacttggcgg cgtaagcgaa gcgctcggag tgccggaatc gacggcgctg cggctgcttt
    20041 tgctcgcggc ttctgctcag cttgtgcagt ttggtttcga cgtgttgtgt ccttcgtgtc
    20101 gcaatcctgc ggcacaggtc gatcacctga ccgatctgac cgacgaggca ttttgtacgc
    20161 tttgtgagct gcggattccc gtcgaattcg cgagcaatgt tgaagtgaca tttcggcctg
    20221 catcgtcgct gcgcaaggtt tcacggccac tttattcaag cgtcaatccg gcgacggcgc
    20281 agcatgtgat cacgcagctg gtgttgccgg ggacgaccaa gctgacgatg acggtgccaa
    20341 acgaaccggg cgagtttcga ctttgggcgc ggggcggggc aacgggaacg ctccgggtga
    20401 cgacgcttgg accgagccag gcatcggtga ccgtgggtga cacgatggtt cccgccgagg
    20461 tcgaagtcgc gatgggcggc acgctggaaa ttgaccacca gaccgggcag gccagacact
    20521 ttaagctgga gcgacccgat tggaagcagc agcgactcga cggacatcag cttacgctgc
    20581 atccgctgtt tcgacgtctg ttcccacatg aactgccgaa gccatctgtg cagctcgccg
    20641 taccgacggt ggcgctttgg cgttgtcgtt ttgcttcgct cggcgagctg tgtgctgcgc
    20701 tcggtgacag cggtgcgttt cggcagctac aagactgtca acaagcgatg cgaaaagccg
    20761 tcgatgaagc cgcaggtgca gtgctgcgca gcgatagtga tggactgacg gttgcgtttg
    20821 caagtggtgc cgtcgcgctg cgctgtgcga ttgcggtcga gcgagcgctg cggacgctgc
    20881 gggcagacaa tccgtttgcg catctgcttt tgctcaagtt cggtctgtac catggtcccg
    20941 cgacggtggt tggcgtcgac aagcggcttg actactttgg tcaaacggtg gcggtttgtg
    21001 accggatggt gagcgaggct catccgggcg acatcttggt tcccgccgag ctgctgtccg
    21061 atgtgtcggg gatggaaggc atcaaagccg gaccgatctt ttccctgacc gggcgcgggc
    21121 tttcgtctcc cgtggcggtg tcgcgtctta ccgttgagtc ttaggcggag tcttttcact
    21181 gccgatgcaa agcacgacgc ggccacgcgg gcggccttct tcgacgtgac gatgcgctgc
    21241 ggcagcttca gcgagtggaa acacccgatc gatgatcgga cgcagcttgc tttgatcggc
    21301 aagctcggcc aaaagttcca gatcggcgcg tcgcggctgg gcgagcggcg gcgggacagt
    21361 ctgcggaccg ccaaaaaaac gactcgtcac gctgtcgagc agcgtgcgca aagacggaac
    21421 aatcggaata aagcgccctt tgggccgcag catcttgcga cagcgatcga gcgtaagctg
    21481 tcccgacgta tccaggacga catcaaaggt tccggtcagc gtcgccaagt cttctttttc
    21541 aaagtcgatg acttcatccg caccaagaga tgcgaccaat tcgctgctat cgccggaaca
    21601 aacgcctgtg acgacggctc caagcgcttt gccaagctgg accgccagac tgccgatgcc
    21661 acccgcggca ccatttacca acagcgcttg tccaccttgc agtcgcccgc gatcacgaat
    21721 cgcttgcagc gccactgtcc ccgataacgg cagtgctgcc gcggcttcaa acgacagctt
    21781 cattggtttg cgcgtcaccg catcggcagg cagcgtcaca aattcggcat aggtgccggg
    21841 attttgtaac gcccgcagcc caatcacttc ggtgccgacg gtcagtttgc tgacgccttc
    21901 cccaatcgct gcgatgatgc cgcagacatc gtgaccaagg atctcgggtc ggcccccagg
    21961 aatgagccgg ctcacctgac cagcgcgctc ttgacagtcg agcggactga ccgtcgtggc
    22021 gcgcatctcg acaagcacca taccgcgacc cggcgtcgga cgaggccgct ccaaaaagcg
    22081 cagcacttcg ggcggtccga cagaaggcag caccaccgca tgcatgttca tcgggggctc
    22141 cttggttcga tgtcgattcc gcgggttcgc ataccagagt tcctaacggg gcgaatcgtc
    22201 cttgcgcagc gcccacagca ctttgcgata gacctcttcg ctcatcacca gcgacgaatg
    22261 accaagcggc accgtcacac catgcgcacc ttcaagctgt gtcgcggacg gtggacaaag
    22321 ccagtcgcgc tctgccgcaa tcgtgaaaaa ttcgacttca tttggcagcg gcccggcatg
    22381 taactcctcc aggaagtcac tgccgggaag tagctgccag ctcgacgccg acaaaagacc
    22441 catcgtcgca accccgagtg ctgccaccca ggttcctcgc gcaggggttc caagcatcac
    22501 aatccgccga acctgttctt tgccgccgag tcgcttgatg taataaagac caatcagtcc
    22561 gcccatcgag tgagccacga tgtcgatgcg acggatgccg gtctgttcgc agatcgtctg
    22621 caccttgcga tggatcaaaa gcgccgatgt gcgaatgtcg cggacgttga gcatcccaag
    22681 gttaaatgaa aagacgtgga aaccgtcgcg gagcagccgc tgctccagca caaacatcga
    22741 cccgcgcgtc cccaaaaaac cgtggatcag cagcacgggg ggatgattgg gcgaaagctg
    22801 gtcgggaaag cggcgctgcg tcatttcgtt gccgcgtacc aagtgacgca gatacgaaaa
    22861 ggcatgctgg ccaccgatga gatcctgttt gaggttgtct ccatccagtc gacggacttt
    22921 gcggtggtcc gtaaccatcc gacggacgcg cgacagaaga cccctttccc catccttgcg
    22981 atttgattcg tccatccctt gtctaccgag cgtcgattcg gaagcagcca gtctttttag
    23041 tatcgcccaa tgtcgctacg ttcgcgtagc tggctcgcaa aagcgcgaac ttacagcaaa
    23101 ctgcggcgcg gtctagggcc aaagcggcag gtcggaaacg aacaatgccg ccgcaattct
    23161 ttgcaaagct actttccacc gccgggttat aacaccgggc gacggctgct ggccaaatcg
    23221 gtgctccgca attgatcgct ttacgaccaa gtttcgactt gagtcggtga ttcgctgcga
    23281 tatccgctgc ttgcgtggtc ttgtgtgcag ggctagcttt gaattggacc gaccaataaa
    23341 ccagtgaggc tatcatgagc gcactctcgc agaatccaga ttccagccac gatgagttgg
    23401 ttgcgctgcg ggtggacagg ccaacctacc cgaagacctt tcttcgcagc gatgagcgtc
    23461 aatcggcctt ggcattggcg cgggcgatca tgccggaagc gggtgggctg ccagaagcgg
    23521 ggagcgcgac cgtgctgcgc gccgaaaact ggctggctga cgtaccgatg gccggggatg
    23581 gctatcgggc tctgctgcgt ggtttcgatt tgtatgttcg cgcggcgacg ggaaagtatc
    23641 tgcaatcggt cgatcctgaa cgcctgcgag agctgtgtcg tcgttttgcc gaaggcgaca
    23701 ttgggcgacg ctattttttg tacacgctga cggcaccgct caagggcgcg tacttcgacg
    23761 atccggcgat ctatcgtcat tttggcattc catatcgcgg cgcaccgtca gcgataccgc
    23821 agacacttgt gccaaaagaa ctgccgcgct gggcgcatga gcggactcag tctgcgtgcg
    23881 aagtctccga tggcgaggtg atcgactgtg acgtggtggt gattggttca ggcgcaggtg
    23941 gtgccgtcgc tgcatatgaa ctagccaaag cgggtcgcgc ggtcgttctc ctcgaagaag
    24001 gtcagtactt cggtcgtcgc gactttgccc atagcgcttt ccatctgcaa aagcatctct
    24061 accgcgagct gggcgtcacg atggcggtcg gcaacacgtt tatcccgata ccagtcggtc
    24121 gcaccgtcgg tggcagtacc actgtcaact ccggtacttg ttaccgcatg ccgtccaggg
    24181 tgtttgccga ttggcacaat caccacggcc tgaccgagct atctcccgac agcatggacc
    24241 ccttttatgg ggcggtggaa gatgtgcttg gggtaaagca agcgccgcgc tcgcttttgc
    24301 aaggcaatca gcccattgcc gacggctgtg acaagctggg tatcagccat cacggaccgc
    24361 ttgcgcgcaa tgctcccgat tgtgatgggc aaggactctg ctgttttggt tgtccgacgg
    24421 atgccaagcg gtcgaccaat gtttcgttcg tcccgatggc gctgcaagcc ggggcgcagc
    24481 ttctgacttc cgcgcatgtc gatcgcgtga tcatcgaaca cggtcgtgcc gttggtgtat
    24541 cggtggtgtc gacgcagccg gcggggtctg gtgggcgtcc gagacgcttt tcggtgcggg
    24601 cgtcagccgt cgtcgttgcg gcgggatcgc ttatgacccc ggtgctactg ctgtctgatc
    24661 ccgtgacgcg gcgagcgctc gggcgctcag gggcgctcgg caatcacatg acgattcatc
    24721 cagcgattgg tctgcgggcg gtgatgccga cgaaactaaa cagccacatc ggtgtgcccc
    24781 aaggctacgc agtcgaagaa ttccacgatg aaggcatctt gatggaaagc gcctttttgc
    24841 cgcttgagct gaccgctgcg tcgatcacct tggttggcaa gccgctcatg gagctgatgg
    24901 gtgcttatga tcggcatagc tgcttcggtt tcctggtcga agacaaaggc caaggcagtg
    24961 tcagacccgg ccctggcggg cgaccgctga ttcactacaa tctggcggac cgcgatgtgg
    25021 ctagacttca gcgtggtgcg tcactggttg cgcggatgtt tttggctgcc ggtgcagagc
    25081 gcgtcttgac cggcatctcg gcgctaccca tgctttcgtc tcacgatgaa gtggatcgac
    25141 tctcggggct tcggctgcgt ccgcgcgact tcgagctgac tgcttatcat ccgcttggca
    25201 ctgcacgaat gggtcactcg ccacaaaaca gcgtcgtcga caccgacctg caatcccacg
    25261 atgttccggg actttacatc tgtgacggga gcgtgatccc aacttctccc gcagtcaatc
    25321 cacaggtgac gatcatggcg ctggcgatgc gggcggcgcg gcgactggcg gaacggctgt
    25381 agcttttgct tcggcagcac gtcgcgcgtg cgccgattct gcgacttcgc ccagcttctt
    25441 gagtccccgc tcgaagtcgc cgccgaccat cttgtccatg ttcataaaca cggtcatcgc
    25501 cttcatcatg aagtttgctt taccgctcat gctccaggtg actttggtct tgtcgccgtc
    25561 gggagtgaag gcaaaccgcg tgtcattggt ccccggccac ggcttgataa attccagctt
    25621 gatagaaagc tgcgacggcg gatttgcatc gacgatggtc atccgacctg atccgacttt
    25681 gtcgttgcct tcccaaaagt agctggcacc tgggcgattg ttttcgcctt cgtagacctt
    25741 cttcatcgac ggatcgagcg catcccacgg ggaccaagca ttccaattgt ggaaatcacc
    25801 aacctgcgtg tagatcacgt cggcaggtgc agaaatgctg gcgctgcgct cgacgtgaaa
    25861 tgcatccggg cgactcgcga caaaagcaga caacgcagca acggttagac cgagcgcaac
    25921 agcaacatgt ttgagcgtga acatgacgga cctccgggtg aaaaagaggc ccgaccatac
    25981 tcgcgccgca cagtcagcgc aaagggggaa gcttggttaa aaccagactt tgtagccaag
    26041 tgccgtgcag cgaccgcgga caaattcctt cgatgcagga tcgaggtgcg gcacgacgta
    26101 ttcaaggatg tcgcggctct gcaagtgaca cgcggaaacg ccagcaatgc gccaggcgag
    26161 tggactgcga atcggcaccg atgttgaggt atgcgccagc gcaaccgctt tggtaaattc
    26221 cccgtgctgg tgggcgatct gcgcatcgcg tagatagcga tcaatttcag cgaagttgtt
    26281 gcgctgcttg tcgtattcgc ccgcgttgcc aagcggtttg ccgtcttgat ctcgcagcgg
    26341 tggataaccg gccagctttt tagcccctgg cggctgtccc gtgatgagcg cacagggccg
    26401 ccatcgcgcc gtcagtgcat ccacatgatc gggcgctacc tcgagaatct tggcgacatg
    26461 cggttcgatc ttgtcgcacc gacccagcga gcgttcggct tccgcttgtt gcaggtgata
    26521 cgcaacgaca agctgctgaa gaagatcgta atgctgctgc gcagtgatgc ggaagctggt
    26581 gcgcggtggc agctcgcggt tgcggctcag cgctccgtcg tagtctttgc tggcgagcgc
    26641 ttgcatgatc tgctgcactt cactcgacag ctttttcccg cgctcaaccc gctgcttgat
    26701 gttttccgca gcggtagaaa cgtaaggtgg ctgacctggc accgactggg cttgcgttgc
    26761 cagtcgaacg gcatcttccc accgttcttc catcagcgct tcatcggcca tcaccagcat
    26821 ctgtccgact tggcctgatc gtttcttgcc ctgcagctcc cgcacgagca gtggcatgac
    26881 aaaaagaccc gccatgcctg ccccagcaag tccaaacagc aggctgcgct tccacgaaaa
    26941 tggcggtgct gccggcggcg gatcgagtgc ccgcagaaag tcgagcatcg tcgggaaacg
    27001 atcctctttc ttcttcgaca gcgctcgctt gattgctgcg gctacatgcg gcggcacggt
    27061 cacttcaagc tgctcgatcg gcgtcggtga ttcgttgatg acctgcttaa acaggtcgat
    27121 cacgttttcg tgatcaaacg gcagcttgcc cgacagcatt cgatacagga tgactgccac
    27181 cgaccattga tcggaccgac cgtcgatctg cgagttttgc cccatcgcgc cttcgggcgc
    27241 catgtagcgc ggcgtaccga gcacgagcag gtctttggtg gctttgtccg ccggacgcgg
    27301 cactttcgat acgccgaagt cgatgacttt ggcggtctgg atcacttcgc cgtgttcttc
    27361 gtgatgcgac aggaagatgt tgcgtggctt gagatcccgg tgaatgatcc cgcgctcgtg
    27421 ggcagcatgc agtgccgacg agatttgccg ggtgatctgg agcgcttctt ccaggggaat
    27481 tttgcccgtg cgttcgatcc ggtcttgcag gtcttcgccc tcgagcaatt ccatgacgat
    27541 gaacgccatg cccgatcggt cacgatcgag gtcgctgacc tgcacgatgt tcgggtgacg
    27601 caacccagcg atgattcgtg cttcgtcttg gaaccgctcg cttaggtcgc cctgcatcgc
    27661 cgcgcgctgg tgcagcactt tgatggcgca gacaccgcct gtacggacgt ggcgagcccg
    27721 aaacaccgca cccatgccac ctgtcaccag gagttgctcg agccggtagg tcccattgag
    27781 tacctgtccg acgagcttct cggcttcctt ctgttcttgg gacacggaca tcgcctatcc
    27841 gaagtctcgc tgactttact taggtctaga ggctatcaaa gtcgcccacc tcacgaaagg
    27901 ctttcgcgca gatggatgtt tagacgcttc tggatgtagg caactgcctc ttgtcgggtc
    27961 tcgacatggc gcagctcaat atggagctga tggatgatct gtccggctcg cagtgccaaa
    28021 gtctgcgtca cttttgctaa caggctcgcg ccatagacca caatcggcat gtagttgagc
    28081 cctcgattca ctgcctgcac gtagaagttg cgaacttcgg cgctcagtgg ggcaagctcg
    28141 cgaccatcga cgattagcgc gacttttccc gtccgctctt gatacgcgag gagtcttttg
    28201 accagctcct ctgcgtgggg cagtgcgacg acaccgtgcg gttgccactc gcagtggcgc
    28261 tcgtattccg cgaagcggtg gggaccgatg gagacccaag ccgtgcttgc atctcttggt
    28321 cgagtatcgt ccatagcact tcgatcgtat cagcgattcc aggtatgcgg ctaggctcag
    28381 tcgccgctcc attccggtgc attgtcgtct ctcggagggg gacggcggct tggcacgagc
    28441 catacgacga caagtgcgct cagcggcagg acggccagat acggcattgt gtttgtccag
    28501 ccttgcgcat cagccagcat cgacataagc ggcggtgacg cgataaatcc gatcctgccg
    28561 agcaaactgt tcgaccacgc catggcgtcg ccacgttgtg ccgtgggaaa tagctctgtc
    28621 gtgaacgccg tcgcgatgat gagtccgccg ctcgcgccac ttgtcaaaca tgccagtgcc
    28681 agacgcagaa gtggccctgc tggcaatagg tacgatccta gaatccccag cgtccacagg
    28741 ccgtacacga tcaatgcgcc gcgtcgcttt cccacccgat tgagcagcgg tccgacaaag
    28801 cccgacattg gaatggcaac cagcgcagcg agcccgagat acgttcccgc taccgctgcc
    28861 gacagcgaca ggtctgcaac ggcatgctct ttccaaaacg tgatggtgct ctggtttgcc
    28921 gcataggtaa gaagccacag gaaagagcac agcagcaggt tgcgtcgatg ctggaccgtc
    28981 cacatcgcag taagagatgg tgtgactcga cttgtcagac gggcaaagct cggtgtctcg
    29041 tgcagatgca aataacccca cggcaccgtc agcagcatca ccgcccccag caaatacaca
    29101 ctgcgccagc ctggacctgt gcccatcagc ttgggggcca ggagggcgca gacgacacct
    29161 ccgaacgcgc cggttgcaac cagcaggctc agagcgctcc ggactttgcg tgccggtaga
    29221 ctttcagaga tgtaaagaga ggctgccccg agggcactga ccaaaaagat ccgcgccgcg
    29281 aactgcgtca gaagaaacaa cgcagccgac ggggcgagcg cagacgtcag cgaaagcagc
    29341 gaatacccca ggacggaaga cagcaggatg ctgcgtcgac cgactgaatc ggcgcggcgc
    29401 aaaagaaagt aaccgagcag ggtgccactg ttggcgaggc cgacaagctg tcccccttcg
    29461 cttaccgaca gtgcgaagct tttacgcagt tcgggcagcg tctgcgaaag tgcgaccaca
    29521 tcaaagccat cccacagcga caccatcagc agaaagacca gcatcggcat cggcagtcga
    29581 tcgatttggt ggggcgcttg gcttgtctcg ttgactccct tggtcggtgt aggctgcaca
    29641 gccctttcag cataacgagc cagtgcggag cctgtcattg tgggcgtttt gttcgcgagt
    29701 cgtactttcg caggacgccc catggcgcat aatgacgtcc atggaagaga tcaggctctc
    29761 ggcggtcatc gggaactcac aacggctcga tggcggcgcg atgtttggca atgctcccaa
    29821 ggcgctgtgg acccgctact tcgcacccga cagtgaaaat cggatcagtc ttgcgtgtcg
    29881 agcgctgctc gtcgaagtag gggcccgcaa gatcctgctc gaaacgggaa tcggtgtgtt
    29941 cttcgccccc gaaatgcgca gccgcttcgg tgtcgtcgaa gaagatcatg tgctgcttcg
    30001 gtcgcttgct gcgctgggcg tcgcagaaga cgaaatcacc gacgtgattt tgtcgcatct
    30061 gcactttgat cacgcgggtg gccttttgtc cgcgtatgtc ggcggccaat cagcgcggct
    30121 tctgttcccc aaggcccgct atgtcacaag tgcgtcagcg ctggctcggg cggaaaagcc
    30181 gcatgtccgc gacaaggcat cgtttatccc cgaacttcag gggctcttgc gcgcaagtgg
    30241 tcgactgcat ctgattgcag acggtgcaac tcacgacgag acgctgcccg gactgcgctt
    30301 tcatcactca aacggtcaca cgccggggct gctcctgacc gaagtctgtg gtccgtcgcc
    30361 agatcgagcg gtggtatttg ctggcgatct ggtgccaggt actgcatggg tccatctgcc
    30421 gatgaccatg ggctatgatc gctatcccga attgctgatc gacgaaaaga cagcgttact
    30481 gaatcagttg tgcgaccgag gtgcttggct cttctacacc cacgatcccg tcgtggcgat
    30541 gagcaaaatc agacgcggcg aagcaagccg tctgtccgcg gacaagcccg aaacatcacc
    30601 cactcgcttc gttttgtaag ccaacgtcaa ttccatcact cggtctgtga tctcgatgtc
    30661 agccggaacc actggcatcc atcgctgatc caaaccgaac aaccaatgag tgattgggat
    30721 ctctcacaga gacaatccaa tctcattggt tgagatcctt atcattcaat aagagatccg
    30781 tttgcttcgc gctgacagtc caatctgtcc cagaagcagc gcgatctctt gctgcgaact
    30841 caacgtcctg caaatgactt gcaatttctg ttttcacgtc ggtgaagtcg tttctgctta
    30901 tcaccggatg atgaaaaact tgcagttttt gtcgttttgt gtgggttcgg gtggcaggtt
    30961 tggaatgctt tgctaggctt tttgcgcacc ctttgttcaa cccgttttcc aaggagtttg
    31021 tgcatggctt cgcgtgtccc tgttccccct cgcagtttcg atgatcagct agagtccctg
    31081 atcgcttttc tcaagcaatc caagaccgcg cttgttggcg ttgatccgaa gcagctcgaa
    31141 tcggacctaa aagcccagcg tgaagaacga caaaaagatc acgatgccga acggcagtac
    31201 gagcagatcc atcgtcggtt tttggaatcg caggctgaac ggtatcgtcg cttcggcgtg
    31261 gccgtgcaag tgatgcgggc cgccaatcgt cacaacgccg acgtgctggc ggcgatggag
    31321 cagttcaagc gtccacgaac ggcaacttct gcgaaaaagt ccaaagctga caagccgtag
    31381 ccggcagaat atagaagaca ggtacgcatc agcttggagg gaccgagatg agagaagcac
    31441 tgggttttgg gctggggatg ggcctggcga caagctacgt gctgcttctt cgcctgaacg
    31501 cccgtctgta tacacaaaac agcgggcagg gtctggcgct gcttctgcac gtcggtcggc
    31561 ttgtcgtgct ggtcgcggtg atggtgctgc tcggttcgct cggcaagcgc tgtcttctgt
    31621 atggactgct gggttttgtg ccgacgcacc tgctgttgta tttgtggctc cggagacagc
    31681 ccgatgacat cgcccctcct tgaaccggcg ctgttcaaca tcgggcccgt accgattccg
    31741 gcgtcgcttt gcaccagcgt gggcatcacg gtcgtgcttg gggcgggcag cttcattctg
    31801 agccgtctgc ttcgccccga gccgggcaaa gtccaagcga tgtgtgaagt cgtcatcacg
    31861 acgatccttg aacagatcga agaggtgacg cgcaaagacg gtcgacccta tttgccgctg
    31921 attgggacgc tgtttctgtt tctgatcgtc gccaattcgc tgtcgctgat tcctgggcta
    31981 cagccgccaa cggaacgact ggagacagtg atagcgctca gtctgacggt gtttgtatcg
    32041 gtttactact acggcatccg tcggcacggg ctgttttcct atctgaaaca cttttttcag
    32101 ccaacgccgc tgctgtttcc gattcatgtg ctgtcggaac tgacgcggac gttttcgcta
    32161 tctatgcgtc tgtttggcaa catcatgagc cacggcttga tcctggcggt gattgtgtcg
    32221 gtcgcagcgc tgcttgtacc gatcccgatt cttctgtttg ggcttttcac cggcgccgtt
    32281 caggcgtaca tctttaccat cttggcgacg gtatacatcg ctgcggcagt cgaggatcat
    32341 ccgcaagccg taaagaccgg ccacaaggag tgacatagaa catgagcgtt caggtcgtga
    32401 gcatcctcgg ggccgtcatc gctgtctgct ttggcgcgat tgggccggca ctggccgaag
    32461 gacgggccat tcaggctgcg atggaagcga ttgcacgaca acccgaagcc ggaccaacga
    32521 tttcgcgctc gctgttcgtc ggtctggcga tgattgaagc gatggcgatt tactgtctgg
    32581 tcgtcgcgct tgtgcttctg tttgccaacc ctttcgtcaa gtaagcgatg cacgtttcgc
    32641 tgctgcacat cgcgtttcag acgatcaact ttctggtctt ggtgtttctc ctccagcggt
    32701 ttttctataa gccgatcctg ggggtgattg ctgaacggaa gcagaagatc gcgcgcgagc
    32761 aagaactggc tgcgtcggaa aagcaggcgg cggaaaagct aaaggcggat cttcagaaca
    32821 agctgcaagc gatcgaagac gacaagcagc gggtgctgca gcaggcgctt gtgcaagccg
    32881 aaacgcaagc agccagtctg cggcgcgaaa tggagcaaaa gatcgacgga gagcgtgctc
    32941 gtcagacggc gcttttgcaa agcgagcgca gtgaggtcga gcaaaagctc aaagagcagt
    33001 cggtgcggct gggcctgctg attgctgagc ggctgctgcg cgagggggca gtggtaagtg
    33061 atccgctcgt cgccgtccac agagcgcttg ggcagatcga ggcgcttcca tcgtcggagc
    33121 aaggtcgcgt ctggacggag cttcgccagc atggtgccga gctgaccagc gtggacgcgc
    33181 tgtcggtgac ccaggttgcg caggtcaaag cagcgatcga atcggcttgt cggcatccgg
    33241 tttcgctgac cacccagact gaaccgaagc tgctttgggg cgtagaactg cggcttggtc
    33301 acttgtcgtt tgcatttcac ttgcacgccg gacttgccaa agtgcagtcg gagctgttga
    33361 ccccatgagt gatcttttgc gcaacctgat tgcgtttgcg caagacaccg aggggaaagt
    33421 gcgtgagctg agcctggccc cgcgaccgga atcgcttggg cgggtgattg cgattgccga
    33481 tggcgtggtg cacgtttccg ggcttcccga agcgcagctt ggcgaagtgc tgcgctttgc
    33541 ttcgggaacg atggggctgg tcgtcgtgct agatgaagac cagatcggct gtgtgctgct
    33601 cggcaaacaa gaaggcattt tggctggctc gtcggttcgc gggacgggcc aggtgctgga
    33661 tgttccagtc ggtgaggagc tgctcggacg ggtggttgat ccgctgggac tgccgctcga
    33721 tggtgggccg aagatcgagg cgcggcatca cctgccgatc gagcggcccg caccggggat
    33781 tacccagcgt cagccagtga cggaaccgct gcacaccggg atcacggcga ttgacgcgat
    33841 gcttccgctc ggacgggggc agcgtgagct tatcatcggc gatcgtttca caggcaagac
    33901 cgcgattgcc gtcgatgcca tcctgagtca aaacagcacc gacgtgatct gtatctatgc
    33961 cgcgataggt cagcgcgctt cgtcggtgag ccaggctgtc gccgcgattc gagagcgcgg
    34021 gcccaagtcg cgcacgattt gtgtggtcgc tgcttccgac gctgctgcgg ggcttcagtg
    34081 gatggctccg tatgccgcgt gcgcgatggc cgagtatttt agcgagcgtg gtgggcatgt
    34141 gctgctggtg ctcgacgacc taaccaagca cgcgcagatt catcgccagg tcgcgctgct
    34201 tttgcgtcag ccaccgggac gcgaggccta tcccggagat gtcttttatt tgcattcgcg
    34261 actgctcgaa cgggcggcga agatgtcgac ggatcgtggg ggcgggtcgc tgacgacact
    34321 gccgattgcc gagacgctgg ctggcaacct gtcgagctac attccgacca acctgatctc
    34381 gatcaccgac ggtcagatat ttcttgaacc acggcttttc aacgaaggac aaaagcccgc
    34441 aattgatgta ggcaagagcg tgtctcgggt gggtggaaag acccagcttg cggtgatgcg
    34501 caaggtcagc gaaaagcttc gtctgggata tgcgcagttt ctggaactgg aggtattcac
    34561 ccgtttcggc ggcgcgatgg atgagcggac gcagaaagtg atcgagcgcg ggcgacgggt
    34621 tcgggccgcg ctgctgcagc cgcagtttca gcctctttca acggcgcatc aggttgcgct
    34681 tttgcaggca atcgacgacg gactgctgga tgggctgccg acctctgcgg ttgggctgta
    34741 tcgcgacgcg ctggggacgc ttttggccga agaacacaag cttttggcgt tgcggatcga
    34801 gcaaggcagc gttctttcag agccagaaca aaaacagctg ggagaggtgc tgcgggcggc
    34861 tcttttgcgg gtcaatgcca acgagggcaa ggatgttcgg ggctgatctg gctgcgcgga
    34921 ttcggaccgt gcagcagctc ggggatctga cccaagccat gcggcagctt gcggcggcac
    34981 ggcaaaggca agcggcggaa aactttgctg ggctggagcg gtatgctgcc gcaacgctgt
    35041 cggcacttca gcgagggctg gcgcttttgt cgaagcagca aagtgcacca aaacaaagcg
    35101 ggacttcgca gcggcttttg gtcgtttgcc tgtcggaaca ttgctttgtc ggcgcgctca
    35161 acgaaacgct ggtcagcaaa gtggtccatg tgatggcgga agcgccgtcc cagctgtttg
    35221 tcttgggcaa gcggggactt cggcacgtcg aagaacgtgg tctttcagcc catgaaacag
    35281 cgagcatgcc gacgacgctt ggttcggtct cgcgcacggc cagtgatttg gtcgagcggc
    35341 tgtttgatcg ggtttcgagc ggcgaagtgg ccgagataga agtcgtgttt gcccggctgc
    35401 gcggttcggt aagctacgaa ccgatttgtc agcgcctgtt tccgccgcat gtgttgccgg
    35461 cggcttacga tggtgagccg cctttgcata cgctgccggt ggaaaaactg acggcgcggc
    35521 ttttggaaga gtatgtcttt gcgcaggtga cgtggatgat tgcctcggcg tttgcgtgtg
    35581 agcaggcggc gcgcttttct gcgatggatg catcgcatcg caacctgcaa gagcagcttg
    35641 gagagctgcg tcagcaagag cgagccgaac gacaagacag catcaccagc gaaattctgg
    35701 aagttgcgac ggcgagctcg ctgcttgcgg agtcacatca tgacaaatga cggtgtgatc
    35761 gttcgcgtcc gcggatcggt cgttgacgtt cgttttcaaa gcggcgagct gccagcgatt
    35821 ggtgaagcgc ttacagtgaa gctgggcgga cggacgctga tgctggaagt gcagcaacac
    35881 agcgacgtcg ggcaagtgcg ctgcgtttca ctggattaca cagaagggct tcgacgcggc
    35941 cagagcgcgc agcggaccgg ggcctgcatt catgtacccg tcggtgaaaa catgctcggt
    36001 cggctgtgca acgtcctggg cgcaccgatc gacaacttgc caccacctgc ggaagcgacg
    36061 ccacggaggc ccatctatcg ggcggccccg cctttgcgca ccgaaggcca agcacccgag
    36121 ctgtttttca ccgggatcaa agtcatcgat ctgcttctgc cgctgcgacg tggtggcaaa
    36181 gcggggctgt ttggtggcgc gggcgtcggc aagaccgtgc tgctgatgga gctgattcgc
    36241 gcgacggtgc agacgcatgg cggcgtgtcg gtgttcgctg ggatcggcga gcgatcacgc
    36301 gagggcaatg agctgtggct cgacatgcag cagtcgggtg ttttgaagaa cacgacgctg
    36361 gtgtttggcc agatgaacga accaccgggg gcgcgctggc gggttggtct taccgcgctg
    36421 actgtggctg agttttttcg tgatgagcgc aagcgggacg ttttgctgct gatcgacaac
    36481 gtgtttcgct tcatccaggc aggcggcgaa gtgtccgggc ttttgggtcg gctgccgtcg
    36541 cgggtcggct accaaccgac gatggcgact gaagtggcgg aatttgaaga gcgcatcacg
    36601 tcggtcgcgg gcgctgcggt gacgtcgatc caggcggtct atgttcctgc agacgatttg
    36661 acagacccgg cggtggcgca gcttttttcg catctggatg ccagcctggt gctgtcgcgc
    36721 aagctcgcgt ccgaaggcat ttatcccgcg gttgatccgc tggcatcgtc gtcgaatctg
    36781 ctggacccgc ggcttgtcgg ggtcgaccac taccgaatcg ccgaagcggt gcgcaagacg
    36841 ctcgcgcagt atcgcgaact gcaagacatc atcgcgatgc tgggacttga agaactgtcg
    36901 caagaagacc ggctgacggt ggcgcgggct cgtcggctgc agcgtttttt gacccagcca
    36961 tttctggtga cagaggcttt taccggacgg ggggggcgga cggtgccgct tgcagagacg
    37021 ctttctggct gcaaagccat cctggcgggc gagtgtgatg cgtggcccga gagtgccttc
    37081 tatatggtcg gtgctctcga tgaagcgaag aatcggctgt aatcgccgat cttaggtggc
    37141 gagcaagcgg gacaagcggc gttcgcccat gacccactgc ggcacagcca cagccagcaa
    37201 aagaatcgcc gaaaggcagt aactaatccc gcgctccgtc gagtcgagtc ccggaccaag
    37261 ctgttcggcc tgttgctgca ttgcggcaag aagaaagtag gcaagtccga tcaaaaacac
    37321 gatccaagcg acagcgctga tgaagatacc tgaatatttt tgcagtgtac cggaccggta
    37381 tccgagcgaa atggcgcgta tatcattacg cactgcgaat gaagttacac gggtactgcg
    37441 taacccaggt tgcgctgtca gagcgtgttg ttctgcttgc atggtcaggc tccgtgcttg
    37501 ccgacttttg attgaaatgc ttgcaagcca agggccaagg cgatgcgcct agcagcgacg
    37561 gaaaagcgaa cagtagagca ggacgaaacg ggcgggcgat ttcacaacgc ggcgcgtcag
    37621 agcttctggg ggattactgg aaaatttctg cgactgggcg gcccgagatg cgggtgagcg
    37681 gagacagacc aagcaccgcg agcgcagtcg ccgcggtatc catcgtcgat atgttcgatc
    37741 gaatgtgata gccgcgacgg acaccggggc cagatgcgat ccatgggatc tctcgatcgc
    37801 tcggcagggc gcccgtatga ccacgcgcgt ggccaccatg atctgccgac acaatgacca
    37861 gcgtgtcgtc gagcatgttc gcctcttgca gtgcgcgata aatggttcca agacaggcat
    37921 ctgaggcgag cgcagccttg cgatagctgg ccgtcatcca gccatcggag tgacctgcct
    37981 cgtctgggtc agagaaatgg acgaacaaaa gaccgggctt ttctttggtc aagtagcgag
    38041 ctgcttcgtc gctgactttc ttgcagtagt aaccggggcg cgcgaacacg tcgacggtgc
    38101 cttcgggcat gatgtggcga agcttgggct tgccgacaaa cgcggcggtt tttagtccac
    38161 cctcttcggc ggcttgaaag atcgtcggga cgcggatgta gccacgggtt ttcagccagg
    38221 tgttccacgt cagtccatgt tgatcgacat cgacgcccga tagcatcgag gcatgagacg
    38281 gcagcgtcga tgccgttcgg attgtgcgcg ctttccaggt aaacgatccg ttgcgataga
    38341 gctgctcgtg ccagtacatg ttctggccat agatcagatc gggtcgcatg ccgtcttcgc
    38401 tgatgatgac gacgtgacgt acaccgggtg gatgctctag cccaaccgat gcaacgggat
    38461 gaggttgcga ctgcacaggc acaggcttgg cgatcggcat ggtgctggat gtcgcggggg
    38521 ccgcgtgggc cgtcgactct ttgctgggaa gtcgaaatac cgacgacgac aagagcaatc
    38581 cgagagtgct cacggttgct agccggtaga cgctgtgcct tctgcgcatg aatttaggtt
    38641 accgtcaaga aggctccaaa tacaactaca tccgcatgcc gaaagttcgc gaaacagctt
    38701 tggcttttgg cgcaagccgg ccaagatcat ctcgcgcttt caatgtttgg ggagggtgag
    38761 agacttcgcg atggaaaccg gcgagacaaa ccgcggcggg gatgcgtccc gccatcttgc
    38821 gagcgagccg cggctgcgac ctcccgtggg cgggagctat cttggacttt tgtggcacaa
    38881 ccgcgacttt cgcgccgtgt atctggcgag cctgatcagc ctgggcggcg actggttcat
    38941 gatggttgct ttgtacgatc tggtattgag ccagacgggc agtgcgacgc tgatttcggt
    39001 gatcaacctg tgcttgggtc tgccggcgct gctggtgaca ccgtgggcgg gcagtctgat
    39061 cgacaagacg gatcgccgca agctgatggt gacggtggac ctcctgcgcg ccggcctggc
    39121 gctgattcct atcttggtgg tgtcgcccaa gcttctgccg ctcgcgtttt tggcagtggc
    39181 gtgtctgtcc gctggatcgg gcttttttga tccggcagcg gaagcggcgg cgccaaacat
    39241 cgtggcaccc gaagacttgg gacgtgccaa cgcactgctc ggatcggcgt ggggcacgat
    39301 gctgatggtc ggttcggcag ttggtggact ggtcgcaacc tatctgggtc gcaacgtcgc
    39361 gtttgcgctc aatgggctaa gcttctttgt ttcggcggtg ctgctgtggc gcatcaagct
    39421 gaacttttca gaacattcac accacacggc accacagacg acgctgcgcg agtctctttc
    39481 tgaagcggtg acgtttgtga agtcgcgacc ccaggtgatg gcgctgctga gcggcaaagg
    39541 actgcttggg ctgacgcttg gtctgacgtc gctgctttcg gtgtttggcg aaaaggtgtt
    39601 tttgcgcggc tcagagggaa tcgcgaagct gtttgttgcg cgtggcctgg gcgcggtggt
    39661 cggtccgttt gtgctgtttg ccttgctgca gcgctcatcg ctgcgaacgt tggcgatcgg
    39721 accgtgcatg atggtgtact cgctcgggta ctgttttttg tcgaaaagtc cgtcgctgga
    39781 atgggcgatg gtgccgattg tgctgggtca catgggcggc ggtgcgatgt ggcaagcgac
    39841 gacctacgcg atgcaggaat cggtgccaga cgcactgcgc gggcgggtgt tttcttttga
    39901 tcaggcgatt ttgggtgtga cctattcgct gtcgacgatg ggggttgggc tgctcgtcga
    39961 taggcagggc gcgcggccag tgttctttgt gctgtcgctt gtgttcacgc tgctgggcct
    40021 tttgctgttt gttgtgactc gtcgcttgtg gcagacccac agagtgtgac catgtaggac
    40081 aacgtaagat ttttgacttc cttgtcacac cgaagtagtg tggacccatg aagcccacgc
    40141 tttcctctgc caagcccgcg gaaacctcgt ctcgccgtcc agtttcgggc cgtccagttt
    40201 cgggccgtcc tgcttcgggt cgtccagttt cgggtcgtcc gctcactgtc cgggaaaccc
    40261 gcgcgcatgt tccttcgggt ccgactcggg tggaaaagcg tcgcagcatg cgctttgaca
    40321 aagcgttccc ggtggcgctg cgcagcgatg cgtttggtga aatttcggcg attgcgcgca
    40381 atgtttcgac cggcggcatg atggtcgaag tacgcgaacc actgccgctt gggacacagg
    40441 tgctggtcta cttttcgctg ccggattccc atgtgcgcgt gacggctcgt ggcgaagtga
    40501 agaaccacta cttcctgaac ttcgcggatg gcaaaggggg agtgcgggcg ctgtccggaa
    40561 tgggtctgcg cttttcttcg tttgagagtg attccgacct gacgttggga atgggaatca
    40621 accggctgcg caccgtcctg cactaagcag caatcaggtt gcgttgcggt agaaaaaccg
    40681 cttgagaagc tcggttgcca gcacgtacat cacagtgatt cccacgagca cagcgagcag
    40741 aggaagtggc agcggcgcaa agccaaaaag ctgcgcgtag ggtagatagg gaatcagcag
    40801 atcgattccc gtaatcagcg ctgtcagtcc cagaagggaa cgtgccgggc gactgcggaa
    40861 acagggtcga cgggtccgca ccaccagcgc aaccacaagc tcggtaagca gactttcgac
    40921 aaaccagccg gttcggaaga cagtctctcc ggcttgaaag atcctgagca ggacaaaaaa
    40981 cgtcaggaag tcgaacagcg aactgagcag accaaactgc accatgaagc ggcggataaa
    41041 cttcatatcc cagcgacgtg gtttttgaat cagttcgggg tcgaccgcat cccccgcgag
    41101 cgccatcgaa ggcacatcag acagcaggtt gttgagtaga atctgacttg ccaatagcgg
    41161 cagaaacggc aaaaagatcg acgttgccgc catgctcagc atgttgccaa ggttggcgct
    41221 ggtggtggtc agaatgtact tgagggtgtt ggcaaacgtg gtccgtcctt cgaggattcc
    41281 gcgacgcagc acatccaggt ttgattcaag cagaacaaag tctgctgctt ctttggcgac
    41341 atcgacagcg gaatcgacgg aaatgctgac atcggcggca tgcagcgcag gggcatcgtt
    41401 gattccgtct cccatgtaac caacgacgtg accgcgtttt tttagtgcca ggatgatccg
    41461 ttccttttgg ttgggatcga cttcggcaaa tacttctgtg gtctgcactt tggcccacag
    41521 cgcctcgtcg ctcagatcat cgagctgggc tccggtcagg acttcttctg ctttcagtcc
    41581 gacttcccgt gccagatgga cgttgacggc gcggctgtct cccgaaatca ctttcagaga
    41641 tacgccaagc tccttgagcg cagagagtgt ctgcccgatt ccctccttcg gtggatcaaa
    41701 aaaagtcaaa aatccacaaa acgacagatc gacctcgtct tcgcgatgcc aggttccttt
    41761 tggggcagtg agctttttgg ccacaccgag cacgcgatgg cctgtttgtc cccacgcttc
    41821 ggctctttcc agatgtgctg tgcgttcgga ttctgaaagg gagcaggcgt ccagaatcgg
    41881 aacgatggcc cccttggtga tcatccgcag ctggtctttt tcacgtacca ccaccgacag
    41941 ccttttgcgc tgaaagtcgt agggaatctc atcgaccttt tcgataccgg ttggatcaag
    42001 ctgcgctgcg ttgtcgatgg cttcgtcaag aggattggtc agtccggttt caaagtgggc
    42061 gttgatggcg gcttccgtaa ggactgatgt cgatcggttc ccttccggat catagctgcc
    42121 cgaaagacga acttcaccaa cggtgagcgt gccagtctta tcggtacaca ggacatccat
    42181 cgcaccgaag ttttcaatcg cattgaggtg acgaaccagg actccttttt ggctcatcgc
    42241 ttgcgcactt tgtgacaggt tcacgctcag aatcgcaggc aacatctcgg gggccagacc
    42301 cactgccagc gcaatcgaaa acagcagcga ttcaaacagc gggcgatgaa gtgcgacatg
    42361 caccacgacc acgccaagca ccatgatcag catcgtcaca atcagcaaat tgccgaagtg
    42421 acgcagccct cgatcgaact ctgtttcagg gggacgcaag ttcagtttgt ctgcgatcga
    42481 gccaaaaaac gtcttccggc ctgtggccaa gacgacaaat gttgcgctgc cgctgcgaac
    42541 ggtggtcccc tgaaacaccg catctttgcg ctgaagcgga gggcagagtt ggggctctgt
    42601 ttcgatgtgc ttttcgacgg ggagagactc tccagtgagc agcgcttgac tgacaaaaaa
    42661 atcattggcc aaaagaagca ggccgtcggc aggaatcaga cttcccgcgg cgagcaacac
    42721 cacatcccct ggcaccacct gcgagctggg aatctggaca ctttggccat cgcgcaagac
    42781 ccgcgctttg gcgaccacca gcgacttgag cttttcgatg gctcggcctg catcgaattc
    42841 ccgccaggtt ccaatcgcac tgcttgccgc gacgatcagc agcgcgagac aagcatcgac
    42901 ccactcacct gcgagcagag acacgcctga cgcgaacacg agcaaaagca cgagcggact
    42961 tttgatctgc tcaaacagga gccgaaacac cgacagcttc ttcgctgttt ggagtgtgtt
    43021 ttctccccac tgcgcaagcc gcgctttggc ttcgctttcg cgtagacctt gctctgacga
    43081 tgcaagctca gacagtagct tggcagcggg cagcttccag cgttcatcta cagaccgcag
    43141 catgtctgca ttgtgtacct tgtcgcacga tctgccagag aacaaaacga cggcgaagat
    43201 gccaaaaagc cagacacttc gcagggattg gcgcttccct tcttctagcg gatcgcgata
    43261 tgctaagtcg tactatgccc atccgtgtca tccgcttccc aaagggggac agtgatacag
    43321 agaccagggt cggttgcgca gcctgcgcgc agacactgat ctggcggatc tacggattgg
    43381 ggcagtgagg aaaagcatgt cggaagcgag caatccagag cggggacctg tggcggcgca
    43441 gacactggat gggggatggt tccgcaaaga gcgagagcga attgcggtcg ggcggaaagg
    43501 aattgcgcta cgcctgggct ccaccgaaag caaagtgacg acgctagaga cgcgcaaaca
    43561 cgacgttcct accgagtggt tcggcgtgct aagggagctg ggttttcgct ttcctgccga
    43621 tgtcgcccaa gctgaaacgc ccgtcgcttc ggtcgtgaca gatgtcgtcc aagcggctgc
    43681 gattccgtca ccacccagcg acgttccggt tgtgatcgcg gcagagtttg cggtgtctgc
    43741 aagtgatccg ccttctgctt cagccgacgc tgtgatcgaa gcggtcgtga tagaagccgt
    43801 gccgacaaga gcggacattc cgaccaccgc tgagacagcc acgccggtct ctccggtgat
    43861 tcctgctatc ccgcaagcag agatggaagc gcgaagagaa gagaagacgc ccaatgtgga
    43921 gccggttgcg gaagcgtcgg tgccacagga tgcgcagacg cttgttccaa gcaatcacag
    43981 cgaaccgctt gcatcggcat cactgacgcc attttgtggt cactggatgc gcgagcgtcg
    44041 tcgggaacgc ggcattcgga cccaagacct ggtgacgcag cttcggacgc tgccagctga
    44101 cctttacacg attgaacgtc acaacattcg gctgcctttg cgctggattc cggggcttct
    44161 aaaactcggg cttttgacgg tcgatgaagc gaaagtcgcg acgcgcggac caggcagcaa
    44221 gctgtcgccg atgaatgggc tgtggctgcg tcagcagcgt ggacagttta aactgtctcc
    44281 gcaagcggtc ggggccaagc tgggggtcag cgcgctcgac attcgcctga ttgaagcgcg
    44341 acagtggtcg ctgccaagtg agtggtttcc tgcgctcaag tcgctgttta ctgcgccggc
    44401 ctctgcaaca tcgaaaaagg tcgccagcaa accgagcggt tcggcggcca ttgcagcagt
    44461 gaaaaaagac agcgcagcaa aagcggaatc caaaaccaag tcgactccgg ctgccgtggc
    44521 aacgggcaag acgacaaaag ctgcaccggc tggaacatcg gccaaaaaag tcgaggccaa
    44581 agctgcgacg aaaacggaag caagcaagag cccggccaaa gcccctgcaa agacagaaaa
    44641 accgtctgtc agcactgcga ccgcaaaagt ggctgcacct gcggcgaaac ctgttccgag
    44701 cggcgagcca ggacttgcgg aaacgatcgt aagttaccgc ttgatgctgg gtcagcacgc
    44761 gggtctttcc gcagtcgacg tgctggcgca gattgcagcg gatcttcagc ttgccaaagg
    44821 caaagatgca ctgacgtttg aaaagctgcg tgcagcgatg aaagtgctga ccagccggta
    44881 aacgggcaag ctctgtgggt ctaccaaaca gacccacgca agacagacac cgcgttccca
    44941 aacgatccca acatcaatat gaggaatttt agattcctca tattcctcct attcatgcgc
    45001 ggcggtttga gttgttaggt ctcttttcgt ccgacgacca gcagaaagcc gcccaagcgc
    45061 cgcacacctg gcagtaccga tgcttgccgt tcgaccatcc gcagcgccgg accgagcagc
    45121 ggccagcgat gcgcctgcga aaacggtgtc accacccgaa tcccatgcac ggtctcgatc
    45181 tggatttcgt ttggcagata cgaccgaatt tgcggcaagg agtcgtagcg ggtgtagacc
    45241 gcttcatcat ttatgtccgc agcgatcgcc gtcggacgct tgagccgctt aatcagatac
    45301 cgcagcgaca gggtgttgta aaactcggcg agcagatagc cgcctggccg gagcactcgt
    45361 gtcatctcgc gcatcgtctg ctcgatcggc tcgacgtgcg ccaacacttt gaacgaacag
    45421 acaacatcaa aagaggcatc ggcaaacggc agctcgctta ccgaaccttg gacgacttgt
    45481 aggctgcgcg ccgacgcttt gcgcagcatg cctgaagaca ggtcgacacc cacggcgact
    45541 tttgcgtgct gggtcagacg gtttaggata agtcccgtcc cacagcccgc ctctaacagc
    45601 cgggtgtctt tgttcatgta gcggccaacc agctcacagg tcagctcgtc gatgaaggca
    45661 tgatagccat cgtgtcggtg gcgctcgtaa gtcgccgaaa actcatcgta atagtgcttg
    45721 ttgcggagct tggtcacggc ccttgtttgt actccgccga gcccgctgga atccaccccg
    45781 aaaatcagta agcggctgcg ccctttcctt ggcgcccgtt ccacgataca ctttgctgcc
    45841 ccctggttga gccaggggat ggaactttcg cccttgagtc tccccgtgtc tgacccggct
    45901 ctccatcgtc cgtcaaagcc ttggccgctt ggccttgtgg cttgcggcat gtttggcgtg
    45961 ctgacaaact cgctgctcgt gtcgtcgctg cttatcgggc agccgggcga agtggcggcc
    46021 aaaaaaggca agtcaaaggt cggggacaag cgcaagcagc gcggcgacaa accgcaagcg
    46081 accccagctg tgacaccccc tggcgcggcg tccaatggtc cacttgcagc ggtccgtctg
    46141 gcgtgcgact tgggttcgtc gaatcctttg tgtgaaacgg cgtttcatgc ggcgagcacg
    46201 gcggctgggc gacgatacac gctggtcgat tcggccaagc tggaagcact gtttgcacgc
    46261 gaaccgtcgc tgcgaggctg ccgcaatgac tcatgtcgga cggcgattgc cgaacagctt
    46321 ggactgtggc gactgattga cgtgattgtt cactcgccgc ggcaaaaaga cattgtcgcg
    46381 tcggtgtcga tcttcgatcc agcggcgcgt ggcattgctg ccgacgtcga gcgagagtcc
    46441 aagcgcgatc cggccaagct ggacagcacg attggcgaag cggtcgagca ggtgatggcg
    46501 acgcagcgac tgacggcgca gcttcgcctg gatgttcgtc cgactgatgc caaggtcaaa
    46561 atcaccgaca gccgaggcag tgcgcgcgaa atatctgaac cagagcggac cggcaaatca
    46621 ccgattcggc tgttccttgg tggatatacg gtccacgtcg aaaagcccgg ttttctggcg
    46681 caagatgtgc ccgtcactgt cgcgcagacc ggcgcgtcgc tgcaaattga acttcagctt
    46741 cgcccgatcg aagttcgctt cgaatggtcg cccaaagacg ccatcgtgcg cgtcgacaac
    46801 cggcctgtcg gggctgccta tccggtgatg gaactgtccg aaggtccgca tcgcgtcgag
    46861 gttctggcgc caccgggtca gccttacgaa tcgatcgtgc aagacatcga cgtgcggcgg
    46921 gacatggagc ccgtgcggct tttgctccag cgactgacgg agctacgggt tcaggctccg
    46981 cttggttatt cggtcagcgt cgacagccag attttgccgc ccgatcgttt tcgcgcaaaa
    47041 gggctgcaaa ttgaaacgtc gcacaagacg acgcctggtc tgcatctggt gtccgcgacg
    47101 agctttcgtg gtctgcggct gtctcgccaa gtcgaggttc gtccgggcag cagtgccgat
    47161 gcggtgattc atccgccggc gctgtggccg ggagcgctgc tggcgacggc tggacttttg
    47221 actgctggcg ctggcatcgg actgtatttc tacgacggac gctgcctcga tacggactgt
    47281 ttgtttgtca caaactacgc gccggcaagc tacgcgatga tggcgcttgg tggtgccgcg
    47341 tttctgtcgg gcgtgatctg gcttggctac aacgccgcgc accatccgcg cttttacaac
    47401 ccacagagcc agcgcgtcac catcgtgccc agccttgcgc cccgctactc cggggtactc
    47461 acctcgatta acttctgata cggttccgcc gcttcttacc ctaggagtgg cgatgtccgt
    47521 ccgtcttgct cgtgctttgt ctgcactcgt gttcggtagt gcgctcgctg ggtctggctg
    47581 tgccgcccct ggtggggcga tttgtggcga cgatcgaagc tgtggtgctt ctgaacagtg
    47641 tttgctcatc ggcgataccg aagcgtgtca tcgcacctgt accggggcaa atggctgcga
    47701 cgacaactct ccggtctgtg acgctgcgct gaaggtctgt cggtcgtgct tcgcggggga
    47761 agactcgctg tgtgctgctc gcagtgccgc gacgccacgc tgtctaaaag gccgctgcgt
    47821 cgcttgtcgc ccaccgggcg atgtcgcatc ggaaagcagc gagtgcgggg tttcgtcatc
    47881 ggttctttcg ccaagtcccg tctgtgaccc ccgcagctat agctgccggc cctgcttgcg
    47941 ccacagcgag tgtgcgtcgg gcatctgcgt caaagacggc agcgatgtcg attttggcat
    48001 cccgcgcggt gcttgcgtcc cgacaaaaca agtgcttgtc gtcgaccaag agctttgcag
    48061 cggcacaggt cctgcatttt gtacgctgcg ccaagcgata aaccgcatcg acgccgacca
    48121 tcgctacatc ttgctgaaaa aaggggccgg agcgacggac tttggcgatt tgcagatcgg
    48181 cacccaagcg agccacaaag gcctgacgat ccacatcgtc ggaccgcttg ccgacattcc
    48241 accggaaacc gcgacgagcg agcctcgtgc cgtgctcggt ggggcgccgc tcaaggacgg
    48301 tctaacggtc acgcagtcgt cggtctttat cgatggcgtg tacgtgcggg gaaatcgcgc
    48361 gggcctggtg tgcatcggtg ccgacgcgaa gctttcggtc aatcgttcct attttgccgg
    48421 caatgacacg gcgatcgtca cgtcgacaaa ctgcaaactg tcgcttggcg atagctggct
    48481 tggccggggt ccgcgcgcga gcgtgtttgg agatgcaccc ggtaatgtcc gcggcatcga
    48541 cgtcgcgggc ggtgattttt cggtggtgaa cacggtcttt tccgacaacg gcgattttcg
    48601 gcaagatggc tttggcggga ttcgcattcg ttcgctgtcg gcaggggtgc ggcgctcgac
    48661 ggtggtgaat agcacgctgt atcagcagag cggtctgctc aagctcggca agtattttac
    48721 ttcggtgatg tgcgacgttc ccgtcgggga tcggctggtg ctgctcaaca cactgatgct
    48781 ttccgacaag ccgcttttga caagccccga ggagcactac gtcgattcga cctgtggcgc
    48841 gatggtcagc cacgttggat caaatgatgc gtggctcaat gccgcaaact cggtggtgct
    48901 gccgatggaa gcgatgccgc tgcgcaatgc ggcggcgcgg gatctgcgct tgcctgcgac
    48961 gccgggttcg gaaggtccgg tgctgcaaca tggcggggtt ccgatgattg aagtcggtgg
    49021 tgaacgaatt ttggcgccga gcttcgatct agatggacga tcgcgaccga cgggatcggt
    49081 cgcaattgga gcgttcgagc cggtgccgta acttacggtg gtggctggct gggggcgcca
    49141 ccgggggtca gcggcggcgc catcgttccg cgcgcttcca agacacgaag caggattcgg
    49201 tacatcaccg gcagcacaat cagggtgagc agcgctgccg acagggttcc tccgacgatg
    49261 acgacggcga tgggacgctg ggtttcggca ccgatggcgc gggacagcgc cgccggaatc
    49321 agacccagtg ccgccagcgc tgccgtcatc agcacggcgc gtagtcgact gtgactgcct
    49381 tggatgatgg cttcgtcgag cggttcgccg tgcctgcgcc tttcttctat cgacgaaacc
    49441 accagcacgc cgtttagcga cgcctgaccg acgagcgcaa tgaaaccgac cgccgcggct
    49501 atcgacaccg gcatatcgac cagccaaagt ccaatcgcac caccgacgag cgcaaacggc
    49561 acattgagca gggtgagcag cgccagcgcc atcgagtcaa acgctttgaa caaaagcgcc
    49621 agcgtcacca agagcgcgac cggcaccacc agtcgaagcc gtgccatcga tcgttctttg
    49681 gactcgaact cgccgcccca ttcgacggtc atgccgggct ttagcttcac tttgctcgac
    49741 accgcttgct gggcatcgag aacgaagctg cccaaatcgc gaccgcggac gttcatgcgc
    49801 aggccgatgt agcgccgtcc attttcgcgg ttgagcgtcg cgcgtccgta atcgacatga
    49861 atgatggcga cggcggccag cggcaccagc gcgccacctt tggtcggaat gcgcagcgcc
    49921 gagatttttt cgatggtatc gcgggctgct tcgggaagtc gcagcaccac atcgaagttg
    49981 cgctcgcctt cccacagcgt accgacgggg cttccaccga gtgcaaccgt aataaagtgc
    50041 tgcacatcac tcatcgtcag accgaaccgt ccaagcagct cgcgtcgggg tttgatctga
    50101 agctgcggca ccggcgcgct cttgacgatg cccagatcag aaaccccgcg gacctcggag
    50161 atcgccgcca gcgctgcctc ggcggtctgc tgcaagtctt tcatgtcgtg gtggtagatt
    50221 ttgagcgcga tctgacccat ctgcccgtag atgttttcgt tgacgttgtc gcgaatcggc
    50281 tgcgaaaagt tcaccgacag ccctggcacg accgccagcg aagcgtgcat tttttcgatc
    50341 agcgtaccga ggctgggcgt gtctttgggc cagctatcca ttggctggag cttgacgaaa
    50401 aactccaggt tgctcggcag cgcagcgtcg gtgccatctt cagggcgccc gagctgcgac
    50461 gacagcgact caacctgcgg gaatccttgc aaaagcgaag tcagtcgtgg cacaagccgc
    50521 cgcccttcat cgagcgatac gttcgacggc agcgtgaacg tcaagtacag cgatccttcg
    50581 ttgatttcgg gcaaaaactc actgccgcgg ttttgcagga tgtagccggc caaaaacagc
    50641 gaagccacag cggttccaat caccacaagc ggacgcctta gcgcgccgcg cagcgtcggt
    50701 agatacagcg cttcggcggc ttttaggacc ggcgattcgc ggtgcgtcga tcctttgcga
    50761 taggccagcg tcgccagcac cggcaccagc gtgaccgaaa acagcaagct accgagcagc
    50821 gctgtcacca ccgtatgagc catcggtgca aacatccgtc cctcgacgcg ctggagcagg
    50881 aaaataggca gataagcggc gctgatgatg agcagcgcga agatcgtcgg acgtgcgacc
    50941 tgacggacag cccagctgat tcgttcgagg ttgtcttcgg gcgcatcttt gggtggatgc
    51001 tggagcgtcg agacgatgtg ttcgatgatg acgacagctc cgtcgacgat gacgccaaag
    51061 tcgaccgcgc ccatcgacag aaggttggcc gacatcccgc gtgagtacag gtagataaac
    51121 gccacgcaca aagacagcgg aatcgtcgtc gcgacaatca gcgccgcttt gatatcgagc
    51181 aaaaacacca gaagaacgat cagcaccagc accgcgcctt cgacgaggtt gcgtccgacg
    51241 gtttccagcg ttttgttgat cagttcggtg cggtcgtaaa acggccagac gcggacgttt
    51301 tccggcagcg cgcgggcgtt gatgtcatcg attttggcgc gcacccgagc cagcactgcc
    51361 gacggatttt cgccgcgccg cataaggacg atgccttcga cggcgtcggt gtcgtgaccg
    51421 cgactgtaaa tgccctggcg cggcgtccag ccttcttcga cggatgccac gtctttgacc
    51481 aggatcggcg tgccaccgcg cgtcgtcacc gcgactttgg cgatgtcgat ggggcttgcg
    51541 aacaggcctt cactgcgaat gataagctgt tcggccccac gcacgagcac gccgcccgac
    51601 gcattgaccg acgcaccttg cagcgactct tccagatcgc gcagcgtcag tcccttggct
    51661 gcgagcaagg ctggatcggc gcggacgtgt atttcgcgca caagcccacc gtaacttacg
    51721 acgtccgcga cgccatcgac ttgcagaatg cgcggacgca ccacccagtc ttgcagcgtg
    51781 cgcaaaagca gcgggtcgcc ctggatggtt tcgccttgct tgccgacgga acgcagcgaa
    51841 tagcgataga tttcgccaat cggcgtcgac aaactcccaa gctgcggggt gactcccggt
    51901 ggaagctcgg cgagccgcag tcgttcgagc gtctgactgc gggcaaacag cacatccaca
    51961 ccatcggcaa acgtcagcgt gacaaacgac agaccgaaca ggttgatgct gcggactttg
    52021 accaggccaa aggttccgtt taccgcgcgc tcgatcggaa tcgaaacttg gcgctctagc
    52081 tcctcggcgg gctggccggg atacagcgaa atgatgttga cctgggtgtc cgtcgggtcg
    52141 ggaaaggcct cgatggtcag ctcgcgaaat gcgaacaggc cccagatcag gcacaaaagg
    52201 gtggcaatca gaacggcggc tcggctgcgc agcgcggcac gaatcagtgc ggagaacatc
    52261 ggttagtcct ccacatcgag cgcgttaagc agcaaaaggg cgccgcggac gacgatgcga
    52321 tcgccgaccc gaagacctga acgaacttcg gtttgaccgc cacgctgccg tccgatggtg
    52381 acttcgctgc gtttgaagtg acctggcgca gtttggacaa acaccactgc atcaagtcca
    52441 tcgctgacga tgctcgcgga cgggacgcgc aataccgacg gtgccttggt ggggcttttg
    52501 ccaaagtgcg cttcgacaaa cgagttggga cgcagtcttc ccgatggatt tttggcgcgg
    52561 atgcgaatcg gaatggtctg cctgtccatg tcgacgaccg ccgagatgtt ttcgacctgg
    52621 ccttggccca ggctgtcatt gtcccccgga atgcgaatgt cgacgggatc gccgacgtgc
    52681 aggttgccga tgtcgggctg ggccacgtca gctaggacca gcacttcttc caggtcagcg
    52741 agcgtcacca ccggacgatc tttgccggga ccgacctgct ggcccgtcgc cgaatcgact
    52801 tgaatgaccg ttcccgagcg cggtgccaag agccagtagc ggttgtctgc atcgggcgca
    52861 atcgacatcg acttgagccg cgagtctgcc gtctgcgctt gcagctcggc atcgcgcaga
    52921 tcattttttg cgaccagcag atcattggcc gatgcgccac gcgcttcgac gagcacttgc
    52981 atccgctcgg ccagcgcttt tttggtctgt gccagcgccg ccgacgcccg cagctcacgc
    53041 aggagcgtcg ccatatcacc cgagcggatc agcgccagct tggccccggc ttcgacgtga
    53101 tcgcccagct tgaccgcaac gtgatccagc cggccttcca tcggcgcgta gcttggtgca
    53161 gtgcgcgact cgaccgcggc gatgcgcgca gtaatcggcg gtagcggcag cggttctccg
    53221 atggtgactt cgccggtttc aaacggaatc ggctgcggcg tgccttcgct tagcacgatg
    53281 tcggtcttgg tcagccgaaa cggcgtcggc ggactggttt tctggtgctg tcccttgatt
    53341 gattgcagca cgaaaaatcc cagcgatgcg gtgagcgccc ctgcgacgaa gccaaccagc
    53401 aaactgcgtt gcgaagaagc cggcttggca aaccccaaat cagcccgcgg cgaatgccca
    53461 tcccccgatt cctcggcgcc tcccgattca tccggctcgt cgtcgtgggt cggcggcaga
    53521 tgcgaaacac gcttactcat cgctggctcc ttcttggatt aggtcaagac tgatgcggaa
    53581 caggtcgcca agagtggtcg cttcttgcag caacagctcg tcgagagcgc gctgcgcttg
    53641 gtttaagtcg gtgagcggca ccgcccgtgc ttcgaaggcg cgacggacat cacgcaaaat
    53701 cgcctgaccg cgcggaagca cttgttcttg cagcgtggtc agccgctgtc tttgcgtcag
    53761 aagtgcccgg cgcagcgagt caatccgaac ttgtgacgaa agcaccgtca gccgtttctg
    53821 ttccgcaaaa cgccgcgccc gcgactgcgc cgcttgccgg ctcgcttgac cgtgatcgga
    53881 catggccagc ggcgtgctca gcgacaccga cactgagtgc tgttgattgc ccgacgccac
    53941 gaaggaatcg tacagatagc ccacccgcac cgtcggatcg ggaatccgca gcgcgtaggc
    54001 ttggcggtct tcggccagtg ccgccgatcg ctgggcttca agcgcctgca aatccggacg
    54061 ggcttcaatc tgcggggaca gggtctcggc acgaacaatc cagcgggcga gaaacttgcg
    54121 ggcctcttgc tcatcggaaa atgcggcgca gcggctgccg acaaactgtc cgcaagcagc
    54181 ttgcgcagtc aaaatgtcac cttgatcggc ggcaatctgc tgttccagtc gcagaagctc
    54241 aatttccgcg cggtcggcat ccagcggcgg accaaaaccc gtcttgaccc gctcgcgtgc
    54301 aagctgaagc gccgctttgg actgtgcagt cagccgcaga tccgcagaga gccgcaagct
    54361 ggccaccgcc agatcaccca aggttcgcaa aagctggatc gcttgaccgc gcaccgacga
    54421 acgtcgctgg gcatcggcag cggtcacaag gaatgcggct cgatcgattt tggcactgcg
    54481 ccgcgccaga tctggatgga cgctcaatcc gacgccgtag ttgggaatgt tgcgcagtgg
    54541 atgcggcaga tcgggcggat tcggcgtccc aatcggaatg gttcccaccg cagcgtcgag
    54601 caccggattg tcgatgagcc gactttgccg cgcgtccgct tggctcagat ccacgctcag
    54661 gtgatcttgc tggagcgttg gcgcatgatc gcgaaccacc cgcaaaagag acggcagatc
    54721 cagatccgcc gtgggatgga attcctcttg ggccaccgat tccacgggcg ggctctgcgc
    54781 agacgcgcgc ggcgcggtca aaaacggtgc aaacaagcca acacctagcg atgttgtcgt
    54841 aagccaaggg atgatccagc gggcagggcg aagagatgtg cgcatcgtca atcggagact
    54901 ataacagtct ggccgcgcca caagcagtgg cgtcgcgaag cgcgggtttt gggggacgaa
    54961 cgggcgtggt aatgcgtgtg ctgtttaggg tcccgcgact tcgtacttcg cagtcttggg
    55021 atcgatgccg gcaaacggtc gggtgtccgg aaccacgcga acctgcgtcg agaacttgta
    55081 gggaacgatc tgtccgctcg gatacgcgag ctcgatgaaa aatgcggtgt agcccgccgc
    55141 aggtgccgtg accgtggcct ggtagacgcc gccaccttga tcgaacagct cgctgctctt
    55201 ccagtttttc ccaaatgtct cgacgcggaa gtcgcgcgct ttgggattcg tcgcctgcca
    55261 cagcagcact ttttgcggct tgtcctgggt ctgtacgcga atcgtgtttg cgccttcgag
    55321 cttccaggta aacgtgggcc gcggcctgtc atcgcggaat cccgtcacat aagcgagcac
    55381 gctttccagt gcatcacttc catcgagcga gtgatccgca ttgggtacgt agcgcaggta
    55441 tttgtcaccc ggcagatcgt cccagtagtt ttgcgaacca tcgggcaaaa agaactgatc
    55501 acccgatgca ttgatcacga acttgggcat cgtcagccgc ttgcggaaaa gatacggatc
    55561 ctcgtttttc agcatgaacg ccatctcggg cgtgccgatg tgagaggtga tgtggttgta
    55621 gtggtagtcg tacagcgcct tggcccagaa accgtaggtc tcgacgtgct ggcgcataaa
    55681 cgtatcgacg ttaagcacat caatcacgat cggaatcgct gccgccaccc gcgaatccac
    55741 ggctgccgta agccaggtcg tccagccgcg cttggatgca ccagcgacga taaacttttc
    55801 gacattcagg ttccctcctt cacttgtgcg gaggaattcc tgcgtggtgt ccatcgccag
    55861 cacggcactt ttcaccatcg ggaagcgcgc cgtccaagtc gggtcttggg tcttcatcac
    55921 ctgcgcccac gaaaacgcca cgatgccatc ttcggtgtgc gattttccgt cgtggttcgc
    55981 aaatgtcagc ggttcgttcg gaacctgcga caggtttatc accagcgcat tggttgcttt
    56041 cgcaatctgc accatgtcat ccggtgcttt cgttggtggc gtgccgttgc tgcctcccga
    56101 aatcgccagc actgctggtc ccggcgacag attgtcgggc cgaatgatcg tcaggtcgtg
    56161 ttgccactgc gtgcgatcga cttcctgcgg cgtgcgccac gtcagcgact tcatgtcgat
    56221 gaagtacgcg gtatagccgc ttcccttgtc ggtcatccgc agcttccacg agtagctggg
    56281 atctttggtc ttcacatagc gatcgaggtc cgtcagcgga tcaggctggg tccccggatc
    56341 gggcatcatg gtgcctttgt cacaagccca aagggacagc gccgaaaaaa acaaaagcgg
    56401 gccagaaagc ttgcgcacgc tcgtcaacat tcccgtcgaa aacacgcctt ctgcttctgc
    56461 cttgggcgac tctgtctgtt tgctctcgtt catgatgatg cttgcctcct tggacgcact
    56521 tttttcgcac gtccttgcgc gatgaagcgg gcatcatact gaaaagcgca tgaagaccat
    56581 cattctcctt gccctgtcta acgtgtttat gaccttcgcg tggtacggac acctgaagtg
    56641 gaagagcgcg ccgctgtggc aagccatcgt cgtcagttgg cttatcgcgt ttgtcgaata
    56701 ctgtctgcaa gtgcccgcca accgtttggg tcacggcacc tattccgccg cgcagctcaa
    56761 gacgatgcag gaagtcatca cgctctgcgt gttctgcgtg ttttcggtga cgtacttaaa
    56821 ggagccgctc aagtggaact acttggtcgg ctttggcctg atggcgctct cggtgttttt
    56881 tgtgttcaaa aagttctgac acaaaaaaac gttcaggaat gtttggcaaa gtgcgtttcc
    56941 ccaatcgcaa gatcgaggat gcggtccgcc tgggttccca tcgctgtgcg cagaagccgc
    57001 ggtggttcat ccagcggttc gtcgctcagt tgaaacgtcc cccagtgcat cgggacaaag
    57061 tgctgcgcgc ccagctcacc aaacgcaatc actgcttgct cgggatcgat gtgctgtggg
    57121 ctcataaacc agcgcggtgc atacgctcca atcggcagca gcgcatagtc gatgtttggg
    57181 aaacggcgac caatttcggc aaacgccgcc ggatagccgg tgtctcccga aaaatacatg
    57241 cgcgtcgtcg gtgattcgat gacaaaacca ccccacagac tctggttgtg atcacgcaga
    57301 ccgcgtcctg accagtgctg cgctggcacc agcgtgatgc gcaccgtgcc tccatttgcg
    57361 tgcagcgtca cgtcttgcca ccagtccagc tcatgcacac gcggaagacc gcggctgcga
    57421 aaaaagcccg caagtcccag cggcacgacg tactgcacac ttgggccgag cgcgcgcacg
    57481 ctgctttcat ccagatgatc acggtgattg tgcgacacca ccaccgcatc aatcggcggc
    57541 aagtccgcga gcgcgacacc cggcggcgcc agccgtttga tcgtgaacat ccgcatcgca
    57601 aacatcggat cggtcaggat cgaaagccca tcaagctgca caagcagagt cgcgtgacca
    57661 atccacgtta ccgtcgctgt cttgcgattt tcacgcagcg cccggccatc gtttgggcaa
    57721 gagggcggga caaaaccact ctgcttgggg ggacgggatt tgtaccagcg cagcacttcg
    57781 cgaaaaccag gtcgagccgg ccagtcgggt ggattgacaa agcgatcttt taaccactgc
    57841 tttgattggg aatatcgcgt catcgccgcc ccgatcgtag cagagagtct gtccaaaaaa
    57901 gggccctttt gcagccggct gcaaaatccc ttctcgccgc gcgctgtggt gccgcgcgct
    57961 gtggtaacga tccgacatgc atcgcgtggt ccgtctctct tcggtttctt tcgtccgtac
    58021 agcggtcttg tcttgtgccg gcccgatctt ctgcttcgcg ctgtcttcct gtcttccgag
    58081 cgatctggaa aacgcagttc aaatagagga gtctcatact cctctttatg tgcttgcttc
    58141 acggctgtgg cccgatggtc ccattccggt ctgctttgaa gaactagacc cagcgcattc
    58201 ccaagagcgc ggctgggtcc gcagcgccgt cgaaaatacc tggcagagcc tgtcgtctgt
    58261 gcgtttttcc ggctggggca agtgcagcgt ggggacaaaa ggaatccgca tccgcagcca
    58321 agatgtcggt ccgtacacgc tgggccttgg cactgagata gacgggaaaa cagacggaat
    58381 gagcctaaac tttgcattta cgacgtggag tccgggctgc cagcggaccc gcgagttttg
    58441 catcacggcg ggcgcggtgc atgaatttgg tcacgcgctt ggttttgcgc atgaacagaa
    58501 tcgatcggac acgccctctt ggtgtacttc ggaacagggt tccgatggcg atattaccgt
    58561 cggaagttgg gatctaagct cggtgatgaa ctactgcaat ccgcgctgga ccggggatgg
    58621 aaaaccaagt gaaaccgaca aacaaggggt gcgggcaagc tacggcagag gcagcacgta
    58681 ctggcaagtc gcgcagtctt ttggaacgtc gtttgcggtg ggagatgaaa cacaagatcc
    58741 agccagcagc ggtgccgatg atgctgcgtt tgtcgatgtg accggcgatg ggctttctga
    58801 tctggtgctg tggttttcag cctcgcgcac gctgatggtg gcgcagtcgc agtctggcgg
    58861 tcgctttgct tccctgaaaa cctggtcaaa aggcgaaggc agcgcgaccg atcgactgct
    58921 gtttgccgat gtcagtgggg atggccgtgc cgatgcgttg tttgtgaatc gttccacagg
    58981 ggaatggtcg gtttctgttt ccagcgggac tgcgttccgg acaccttcgc tgtggaaaag
    59041 cgggcacgga atcgggtcaa ccagccagct tgctgccgat gtgaccggcg atggtcgtgc
    59101 cgatgcgatt gcgttctttt ctgcgacggg aaactggtgg gttgcggcat ccacgggtct
    59161 tctgtttggt ggctattccc agtggaaaag cggacacgga atcggatcaa ccagtcagca
    59221 ggctgcggac gtcacgggcg atggtcgtgc ggatgcggtg gtattttttt ctggcagcgg
    59281 atcgtggtgg gtcgcaccgt ctaccggaag cgcattttca agctactcac tgtggcgttc
    59341 ttcgcatggc gcaggatcga cataccagtg gctttctgat gtgactggcg acggaaaagt
    59401 cgatgcagtt gtgggttatg aaaagacagg ttccttcttt gttgcgccgt ctttgggaaa
    59461 cagctttggg acggacgctc gctggctgtc tgggtttggc gcggccctgg ttccggggat
    59521 caatcaggtg ttggtagggg atgccactgg cgatggacgg gccgatctgc tgctgcgctg
    59581 gcgcaaatag gaacccgcaa gttgtcgtcg aaaaacgctc tctgatggtg gcgctggcgc
    59641 atgcaaagac acaaggtggc cacatttggc catcgactct gcggttgcgc tctggaaaac
    59701 gctggatcgg attgctacgg tcttttgcat gacgactaca cagccgcttc cttatgacga
    59761 aattccgatg ttgccagatg gcctgggact gggtccaacg ctgctgtttc ggcgcgatcg
    59821 ctacgcattc atgatgaagc tatcgaccgg cgcaccgctg tgccgtgtcc agctctttga
    59881 tcgcaaagtg gcactggtga cgggggcttt ggaaatccag cagcttctgg tcgaaaacgc
    59941 cggttcactg gccaagtctg cggtccagaa attggtcggc tatccagtgc tgggcgacgg
    60001 acttttgggc agcagtggtg agctgtggcg caagcagcgc aagctgatgg ctccgatttt
    60061 tacgccggcg cagattgcag ggtatggaac cgacatgatg cagtgtgccg cgcgcgagat
    60121 ggccacctat gtggatgggt ctctgctcga tgtaacgcag tcgatgaccc ggctgacgat
    60181 gagcgttgcg ggcaagacgc tgtttggtgc cgatacgtgt tcggaagccg atgaaattgg
    60241 ccgggcactg gccgtggcca tccggctgtt tggcaagcta tcggggtcgc cgctttcctt
    60301 tctgcaagtc gcggtcaaag acggtctttc ccgtctgagc caacatcttc cggacgccct
    60361 tgcgagcttc gttgacaaag gtgtccagca gctcgagtcg ccgattctgt ttggtcagga
    60421 gtccgcggat ctggatcaag cggtggcgct gctcgacaaa tatgttgcgg atctgatcgc
    60481 aaaaagacga cagagtcccg atgcgtcgca tgacttgttg acccgcctat tggcggcgcg
    60541 cgatggagcg gatcagatga cagacaagca gcttcgcgat gaagtgctga cgctgtttgt
    60601 cgccggacac gaaaccaccg cggtgtctct gtcttggagc ttgtatcttt tggccaagca
    60661 tcccgaaatc tattcggacg ttcagcagct ggtcgattcc cttccggcag aaccgactgt
    60721 ggccgatctt cccaagctgg gcaaggtgct ccgggtgttc aaagaagcgc tgcggatcta
    60781 cccaccactg ccgctgtttt cgcgcgacac cacggcttcg attgtcgtcg gtggatacga
    60841 aattcctgcc gggactccgg ttctgattgc gccgcttgga acgcatcatc ggcccgagct
    60901 gtggcccgat tcccagcgct ttttgccgga ccgctttttg ccagaacagg aaagccagcg
    60961 acatcgctac gccttcattc cgttttccgc cggaccgcgc gtctgcatcg gcaatcactt
    61021 tgcgatgatg gaagcccagc ttgtgctgac gacgcttctg cgcaactacg aattttcact
    61081 gcctgaaggg actgtggtgg ttccggaaat cgacagcgcg ctgcgtccca aagaaggtct
    61141 gcacatgcgc atcaaaaggc gaacgccacg ccctgcaaac tgaccatctg gccaccacaa
    61201 acacccccga caacaaaggg aaaagcagat gattgtgcga aacgaaacgg tcgatccgtc
    61261 gacgctgagc gcaagcgaaa cagagtcctt gatttcatcg ctgtatgcgg tgcacagcga
    61321 aattttcgat ggtgtaagcc gcgatcagtt tgcaaagtac gtggtctgtt cgccggcaaa
    61381 acggacccgg attcagcttt cctatggaca cgatggcgag ctggccggct atctggcgat
    61441 gcatgcattt cgtccgctgc cgtccggaaa ggaatgtacg gtgctgcgcg cggaagcggg
    61501 tcttcggcgg gcgtatcgcg gcaattcttc gacggcaggt ttcgcgatct cgaatctgct
    61561 caagaccaag tgggactttg gcggaccgct ctattacttg ggctgtctgg ttcatccatc
    61621 gagctacacc atctttgcca acaacgcgcc tgccgtgtgg ccagcaccgg gtgtggacat
    61681 tcccccagag ctgtttgatc tgatgatgcg gcttggagag gaattccatc tggagcaagt
    61741 cgatccacaa agaccgcttg tgcgcgatgt cggctggatc acccgcgata ccgaagtcga
    61801 gcggcgctac tggcagacct gcgacttgcc gggcgcgcgt ttttacgtcg aacaaaatcc
    61861 ggactttacc aaaggccatg gtctgctaac gctgctgccg cttgatctgg cgtcgacggc
    61921 gaaaatggtg atgaacaccg gaacgtcgcg gctgcgcaaa gagatccgcc gcacagtcgg
    61981 gagtctggaa cgaaacgtcc tgcgcagaac gctcgatgcc cccaccgcag aggagctttt
    62041 gcaaacggtc gaggaagtca ccggacttga tctggacagc gtgcggaatg tcggcgtctt
    62101 gggaacccgt tatccgattg ctgcgcgtca gaccttgttt cgggaaggcg agccatccga
    62161 tgcgatgtat gtcgtgctgg aaggttcgct gctggtgctt ggcgacagtc cggaaggaga
    62221 gctggtcatc gatcaactgg gtgcaaacac actggtcggt gaactggaaa tgatgaccgg
    62281 acagccgcgc gctacgacgg tgcgggcggc gattgatacc cagcttctgc gtctgtccaa
    62341 aaccgatctg gacaagctgt tggcggcaga accgcggctt ggcagtgcgc tgtgggagca
    62401 cattcatgga cgggtgtttg cgcttctgtg tcgacggatt ccgcggcttg cacagctttc
    62461 tcggcacacc caagaggcgt ggttttccgc cgcatcttcg catgtgctca cagagcccca
    62521 gcagttcaca gtgctatcgc ctgcgctgct tgttttgtcc aaaggaaagc tggtggtaga
    62581 cagggcaggg gagctgatga gtgtggctgc accggcgctg attccggtgg cggaagggag
    62641 tgcggtacag gcgacggggg aagcgtactt tgcgctgctt ccaccgcagc ccccatccgc
    62701 ttaaccgatg acgagcggat cgatgcagac gccgacttcc aagatcagat aaagcagggc
    62761 cagcgcctgc ggttccagcg cgccgctcgt ctcactggtg ggttgggtgt gatacgtcgt
    62821 gtagagcacc cgcccgcagc catgctgcac actcgctgtc accggcgacg tggtttcgac
    62881 ccagactttc ggggtgatct tggacatttt gccgtcggca tcaatgtcca tcttgtcttg
    62941 taccgatgca atcgcagtcc acgcatcctt cacttcggac agcgacttgc cctgtgcttg
    63001 cagccacgca tccaggccgc tttccttttt gtagaccgac tggttgccgc caccgccgct
    63061 gcacgcgctg ccaatctgcg tcgattcgcc gcgccaggtg atgtagccag ggaagacttg
    63121 gcgaacgtat tcatacgacc aatcggatac gtagatacgg ccccctttcg cgacaaaatc
    63181 agccaggttg gacttgaccc gcgcgtcggt gttgaacact ccggtgcact tgggtccggt
    63241 gtccatttgg ttggtcccac ccgagcacgg caaaaacagg atgtggtatt tgccaagctc
    63301 tttgggatcg gtcaggagtg tatttacgct cggatacggc gacggcaacc cgagtccctg
    63361 aagtccgtag atggcaaacg cgggcgcatc tttggcgagc acttgcgcct tgccgagcaa
    63421 atcctttgaa atggtggctt tcagtcccat ccgtgccagc accacttcaa tcgggtccca
    63481 tgctccgacg acgaccgcga tcttgggaat gtcatcccca ttcatcttgt cggtgatggc
    63541 aggcagcgtc gtcttgtctt tgggaatctg ctgcgcccct tcgacgatgc ttatctggcg
    63601 gacgcggcgg aacgccccct tttgcacgac cagatagtag ttgcccgtgc tcgcatccag
    63661 ctcgaatgtg ccgtcaggtt tggtcgtggt gtgcggtgtt ccatcgggaa tgtgaacgca
    63721 cttgtcacag tacaccgcat cgggaattgc cgtcggtgca tagggaacca catagaccag
    63781 cgcacccgag attggaatcg tcccttccgg tgccaccact tttccggtca gctttcccgt
    63841 gcttgggttg ggaatcgggc ccggacctgg accggggccc ggaccgggct ttacaccgtt
    63901 tgcagcggga tcatcgctga cctcgcgatc gactgcgcag gcagacagca ccagcgacga
    63961 aaacagcaga agcgcggaag caaaggtaga cagtctcatg ggcggactat agcaatggcg
    64021 gcaaaagtgg cagacaaaat tgtaagcctg ccgcggggcc gggtaacatg gcgtctgcga
    64081 tggcttctaa caatcgagcg gaacaagcgg cgaccgcgac tttattgctg gaaaacgtac
    64141 cgctgcgggg ttcttttgcg agcctggttc cgcttctgtc gcagcgcaaa gtctgtgatc
    64201 tgggctgcaa tcaggggcat tacttgcagt ttgcgggcga cggcagtgtg ggactggatg
    64261 tcagcgctga atcggtcttg gcttgcagcc aaaagggact ttccgcgcgg cgccacgact
    64321 tcaattcgct gccgctgccg cttcccgatg cctcgttcga ggtcgtgctg ctcagccatg
    64381 tgctcgaaca cgtcacgatg ccgcttcatc ttctgcgcga gtgcaatcgg attgtgcagc
    64441 cgggcggtac gttgattgtg ggtctgccga tcgaagacgg tctgtattcc cgtctgcgca
    64501 tggactactt tggcggtggg gaagggcatc tgtttagttt ttcgctgcgc aacttgcgca
    64561 agctgatgac gctgacagga tttgccgagc agcgtgtgat ctgtcatctg ccacggcttg
    64621 ggaatcggtt gtccgcgtgg aatgagcggc tggtccggat ctttggcgac cggctgtaca
    64681 ccctgagtgc ggcgtactgg tttgtcgccc aaaaaatcgc ttcacccgtc gctgatacag
    64741 agctgtcgga ctacttttcg cgctgagcct gcaatgaatc ggttgcgtcc ttttgctttc
    64801 gcactcggct tcctcctttt ggtggaactt ttgctgcgct gcgtgtttgc cgcggatgag
    64861 cgcgtgcttc tggatgcgcg caatccgttt ggatgctttg ccgatgatcc gctgtatcgc
    64921 ttgcagaaag cgcgcgagct acccgagccg gcccttgatg tggtgctgct cggtgactcg
    64981 gtgctggcgt cggtggaaaa tggccccggt gagcgcctga ccgacttttt gccaggcgaa
    65041 gtggggcggg cgctggcgga ttccttgcac ggaaaaaaag tgcgtgcctg gtcgatttcc
    65101 aattcggctg cgcgggctgc ggatcagctt gcagcgctga aaatcctgca tgcgcggctc
    65161 acaaagactc cgaaaggact gcgcgatctg gtggtggtga tcagcagcaa tccgctgttt
    65221 ttctctcggc gttttgcatc cgatgcgatg aactatccgt gtctgtcgta tgcgcttgcg
    65281 gacgatccac tgctgctgcg caagctaaac atcccaagcc caagcaaggg actgtggggc
    65341 gattccgatg cgtacttggg aaccctgttt tcgcatctgt atctgttcca gcagcgccga
    65401 aacgtggccg aaaagtgggt ggggggtccg ctgcgtccgc gcctgcgtga gcttgtcacc
    65461 cacggcgttc gtcgcttgtc cggacaaaag gaatcgcaag tggtggtgga tgaaaagacg
    65521 cgcaaccgtc cgtggtttca gcgcgatcac aagcgcgatt ccttttcggc gcactatgac
    65581 tttctgaaaa gcgattctcc ggcggcgtgg aactggacgg cgacccgcgc gctgttttcg
    65641 tatctgcaca gtcatccaga gctcgcgacg ctggtgatga taaatccgca caaccacttt
    65701 ttcatcgggc cgcttgggga caccgatggc taccggacgc ttatcgcgca gctttcccaa
    65761 gaggcgagca ggcaaggcgt gttcctggcc gactatgatc ggaatccagc gctacagagc
    65821 gaacattttt tggacattga tcacttcacc cgcgacggaa atcttgtcct gtcatcgctt
    65881 atcgctggtg atgtggccaa acgggtggcg gggaactaag ccgtgtcttt gctcagcctg
    65941 acgtatggtg tgctggtgct ctgtaccgtt ccgctgtatg cgctgtgtcc ggcgcgcctg
    66001 cggacgcttc tgctgtgtat tttgtcgctt ctgttttatg cctggggttc gctttcccat
    66061 gccgcgctca ttgttggtct ggggacggtg gtgtttttcg tcggtgagca gctgcacaaa
    66121 aagcggagca agctgcctta tgtgttgtgt gtcagcttgt gcgtggttgt gctctgttac
    66181 tttaagtact cgatcttgat gctgcgcacc ggggcagatg tactaccaag cgggagcctg
    66241 tctgcgctgc tttctgcgca cgcggattcc ctggctctgt cgctgcaaat tccgcttggc
    66301 gtttcgtttt tcacgttcga gtttgttcac tacctaaccg atgtgtacaa aggcaagatc
    66361 agccatcccg aacccctgcc ggtgctgcgg aaacggatca gcgatttctt tctgtttgcg
    66421 ctgtttttcc cgacgctgct ggccggtccc atcaagcgct atcagcagtt tcataacgac
    66481 tcccaacccg cgcagagaga catgctcgcg cagggactgt ggcggattct ttgcggactg
    66541 ctaaagaagg tgctcatcgc cgattcgctc gcgaccatta cgctgcggct caaagcgcca
    66601 gagcttgtca cgccaaccgg tctgtggctt gcgatgtacg cctacgcggg acaaatctac
    66661 gccgattttt ctggctattc cgatgtcgcg attggtgtgt cgctgctgct gggctaccgg
    66721 attccggaaa acttcaacta tccctacctg gcaaagagcc tgcaagactt ctggcgtcgc
    66781 tggcacatct ctttgtcgag ctggattcgc gactatctgt tcattccgct cggcggcagt
    66841 catgttccac cgtggcgggc ttttgtgaac ctgatcttgg tgatggcgct gtgtggtctg
    66901 tggcatgggc cagccggtca ctttgtggtc tggggaattt ggcacggcgc tggcttggga
    66961 atcgaacgga tggtccgcag actgcggggt gattccgcag aaaaggaatc gcgcgggctg
    67021 ccgtttctgg gctggctgtg gacattccac tttgtctgtg tgggctggat tctgtttgcg
    67081 gcaccgtcgc tgcgatctgc gtatgtcgcg ctaggcagaa tgttcttttt gctgcgctag
    67141 cacaggtcgc tctggcatcg gctgcggaac gattcggccc tttggtgttg cttgcgcacg
    67201 cagcagcgct tcatatccaa acatccagcg ttcttcggta gcgtcacggt gatccatacc
    67261 caacaggtga cagagtccgt gaaccgacag atggaccagt tcatgcagaa gatcgtgtct
    67321 ctgttcgttg gcctgtttct gcgcgatctc gacggaaatg acaatgtctc ccagcgtttc
    67381 gggaagcgca aatgcctccg caggcaagtc tggactccgg ggcggatgat aggctggctc
    67441 cgcatctcgc tgcgcaaacg aaagcacatc ggtggggtga tcttcatcgg catacgtccg
    67501 atttagatcg tgtagttcat cgttgtcgga cagcgtcagc gacaccatcg tgcaatcgac
    67561 tccagcggcg gtcatggcac ggcgtagctg ccgcacaagc tctctgcgca caggtggagg
    67621 aaccagggaa cggacagagt tccgtacttg caagctcaac atgtggggtt tgtatcacaa
    67681 gctgcgtggg aatgtgcggc cacgacgatc cgggccctgc cttcggcgtt ccttttcatg
    67741 tctgtgacga acacctgttc gcacttgtgt tttctttgat acagacagcg accgactgcc
    67801 acagcccaca ctgcgcgaac tgggcaaacg gcattcacac ccggcgcact tggtgctaac
    67861 ttttcgacag gggcgggatt tcttgaagga gcagcgcgac acaattgggg gggaggacga
    67921 ctccgccacg gcagccacac ttgatccaac ggcgagcgat tccctgccag gtgatgaatc
    67981 gcgcgttgtc gtgcgaacaa cgcgagtggg tcggttcctg ctgctgcgtg aaattggtgc
    68041 aggtggaatg ggaagcgtgt ttgctgcgta tgatgagcag ctcgatcgca gggtggcgct
    68101 gaagcttcta cggaaaccac gcgctggaag tcgtcgtcag cgcatgatgg tcctgcgtga
    68161 agcccgagcc gcggcgcgga tatcccatcc caacgtcatc gcgatttacg acgtaggtga
    68221 aatcgacgga cagatccaca tcgcgatgga gtacgtcgat ggcgagacgc tgctcaagtg
    68281 gcagaaagag agtcagcgtt cctggcgcga cattttgcac atgtatctgg aagtcggcca
    68341 agccttgcaa gccgcccacc aatccgaagt gatccaccgc gatttcaaac caga
//
LOCUS       DAORGN010000002        68134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_116_length_68134_cov_5.982741, whole genome shotgun sequence.
ACCESSION   DAORGN010000002 DAORGN010000000
VERSION     DAORGN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 68134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68134
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_68134_cov_5.982741"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKL17_00300"
     CDS             <1..708
                     /locus_tag="PKL17_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNK43190.1"
                     /translation="FIVRARDAAGNIDKNTIEVTATTPVPDTTAPTFGGVTGATVAGN
                     TITLSWTAATDNVSQAANIVYKIYQATTMGGENYTTANYTSPAGATSFAVKDLTPNTT
                     YYFVVRAQDEAGNASTNTQEKSGMALTPTFSGQVGPLLSTNCAGCHSGGSPSGMLDLS
                     SGKAYAAMVGVNSSGCVSVKRVAASSAANSYVVGKLNGTAVAGCFVGSKMPPGGGLST
                     AQIATVSAWINAGALNN"
     gene            729..1289
                     /locus_tag="PKL17_00305"
     CDS             729..1289
                     /locus_tag="PKL17_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNK43191.1"
                     /translation="MRNPRDSFCSYCGTEFLRPLRYPRKCQNPGCGAETYANPIPVAL
                     LLLPVLHKERMGLLVVRRAIEPQLGKLALVGGFVEEYETWQQGAAREAKEEAGVTVDP
                     NTLAPYFFSSTEPRPNRVLLFALGQPISSASLPSFVPNHEVSERGLIFGTQGLAPLFA
                     FHLHLLASARFFDEKGSNGPHQFLRL"
     gene            1375..2559
                     /locus_tag="PKL17_00310"
     CDS             1375..2559
                     /locus_tag="PKL17_00310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK43192.1"
                     /translation="MSALSVSVHGSISELQKEAWNALLTDSDNPFVRYEFLAALEQSG
                     CVCPETSWTPCHFAIRRGNTLCAVVPAYIKDGSDGDFSRDWEWAGAAQRAGIPYYPKL
                     VLGIPFTPVCGRRVLHPEGLPPHEISDLFSCVIATAKQIMKRFSLGAIQVLFPPADQQ
                     ELFVAHGLIPRIDFQYHFFNPGYRDRDEFDARFSSKRRNILKRERSAPESQGIQIRTL
                     RAEQLLRDPVHYAKLAHALHRSTVDKLVWGRRWLNEAFYVQLFSTLLAPLELVIAQSQ
                     GDIIAGAFNVASQRRLFGRYWGCFRELPFLHFNVCLYHSIDECIARKTLVFEGGAGGE
                     HKIARGFEPTETHSAHAYADPSFHAALARHVKTESENRRQAIAAWRKNDAVLKPLVPS
                     IP"
     gene            2576..3787
                     /locus_tag="PKL17_00315"
     CDS             2576..3787
                     /locus_tag="PKL17_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNK43193.1"
                     /translation="MSLLRAYGFSLLLLLSIAIGLVIGQGAPAWALRLRPLGDLFLNL
                     VFMLIVPLTFFTVSASLGASPSGRRLTKISTVMLLVFLATSVIAAVWGLGFMYVVHPT
                     PGADIVLGRSSVPLPPAPDFLSQLVATCTTSDFPQLVSRKSMLPLLLFAVGVGLAARS
                     VGEPALPFVRVLQGGSAVFIRLFDAVMYVAPIGLLSFFAATVVDTGSQLASAYLRVFW
                     VYGVVSLLYFGVGFSFYAYLAGGWESVRRLWSQMLRPSLTALGTCSSMATMPVNLEAS
                     RDMGIPKDVCDVVIPVGAVLHKDGSVIGGVLKVLFALSLFHLPLTPGRLLMVIGIAIL
                     VGVVMGAIPSGGMMGELLILSAFGFPPETLPLLAVISILIDPLATLLNATGDNVAAML
                     TARLVPKEQPD"
     gene            complement(3784..4833)
                     /locus_tag="PKL17_00320"
     CDS             complement(3784..4833)
                     /locus_tag="PKL17_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNK43194.1"
                     /translation="MSKSDSHKTFSPSDVGVKNGKLFGLPFDYENSQVVVIPVPWEVT
                     VSYRTGTAKGPQAILEASTQVDLFDPVWPDGWRAGFYMLPEDKAWQKRNAKLRQLAQK
                     CIARLEQGVAGDDPDLLLHQGEVNAASSELNDWLERQAQAHLSLGKTVGVLGGDHSVP
                     LGLIRALATRYESFGVLHIDAHADLRRGYEGFQYSHASIMENVLHLPHVSKLTQVGIR
                     DYCDEEEETIREAGDRVSFFPMRGLRKRNFHGEPWARTCQHIVDTLPQQVYVSFDIDG
                     LDPALCPETGTPVPGGLTWDEAVFLLDMVVDSGRQVIGFDLVEVSGNAEWDAIVGARL
                     LFRLCLLAAQSHPHS"
     gene            complement(4852..5538)
                     /locus_tag="PKL17_00325"
     CDS             complement(4852..5538)
                     /locus_tag="PKL17_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNK43195.1"
                     /translation="MTDIAQHLAEVHAKIAAACQRAGRSPKDVTLLAVSKLQPSTLIR
                     AAYAAGQRDFGENYAQELRDKASELADLPTLRWHAIGTLQRNKVKYVAKSASVFHALC
                     SLAIAEELSCRRQGPPIDVLIEVNVAGEDSKSGIAQSALSPFCEKIRKLPGLRLTGLM
                     TMPPQPEPPLPWTPEDNRPYFAKLRTLAESLGLSELSMGTTADYEVAIEEGATIVRVG
                     RSIFGSRPSP"
     gene            complement(5560..6312)
                     /locus_tag="PKL17_00330"
     CDS             complement(5560..6312)
                     /locus_tag="PKL17_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK43196.1"
                     /translation="MLEFRSVEKSFGSQAVLRGLTLHVKDREILFLIGASGVGKSVAI
                     KHVIGLLRVDRGEIWLDGERIDKLSEHELYRIRRRCAMVFQSSTLFDSMSVVDNVALP
                     LRKHQQMSDTAARKAALEFLDEVHMGEFADQLPALLSDGMRKRVAIARALTLKPDYLL
                     FDEPTTGLDPVSARRVDALIRRLSRSRGVTSVVVSHDLTSIFSIADRIALLYQGRVHL
                     LGTPDEFRNTDDPVVRQFVSGHSQGPMETPGF"
     gene            complement(6315..7826)
                     /gene="glgA"
                     /locus_tag="PKL17_00335"
     CDS             complement(6315..7826)
                     /gene="glgA"
                     /locus_tag="PKL17_00335"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNK43197.1"
                     /translation="MNVLHVASEVAPFSKTGGLGDVLWALPKALAQQGAGRVTVVSAR
                     YGSIDPQQHGLAKRLRKLAVSVGGQSFEVGLYEGRLPGPGPQVMVYLIDHPLFADRPG
                     VYGPPGNPGEGYPDNALRFALLSQAAICLAKELTPRPDVIHAHDWQAGLAVYYAQKAD
                     WDTPPATVFTVHNLMFQGLFSLAEARHLGVRDDLLTPDGIEFYGQASFIKAGLQLADR
                     LTTVSPRYAEEIATPEFGYGLDGVMRARSARLSGILNGVDYDRWSPWSDGALPARFGK
                     PAAELDALSEDAFAADLVGKARCKAELQRELSLPLRPRTPLLATISRLTDQKGIDLLI
                     EWLESELTQRTEFQFVLLGQGDPAFEQRLRMLSERFPTRLAVRLAFDEGLAHRIEAGS
                     DFFVMPSRFEPCGLNQIYSMRYATVPIVRATGGLYDTVVDFDARSRSGTGFTFQPFSV
                     AALGHAVRRALSAYHSDEQSFLGLQKRAMRADFGWAASARAYLNVYRQAQSGK"
     gene            complement(7849..8484)
                     /locus_tag="PKL17_00340"
     CDS             complement(7849..8484)
                     /locus_tag="PKL17_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43198.1"
                     /translation="MKVITLQEIQQAFAYLVSKPDYLLRSAVNATRFRFGLPMDAVTW
                     LLGKLSRGKLPEDLSLVAVPPGVRFSATFNVMGTALFVSAVVSVDGVQLSAESIRVNL
                     RIRELQVKPPADSPMAAMLMMMDLSKPGDMLAFLPFRPQVILSAAGDEFVLDLIQLPK
                     LKTNAMAKRVVAAVSEILTIREFATEDDMLVIGLRAMPSGIVSALGHLRGA"
     gene            complement(8604..10877)
                     /locus_tag="PKL17_00345"
     CDS             complement(8604..10877)
                     /locus_tag="PKL17_00345"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNK43199.1"
                     /translation="MTAWTLGGLGLLSSQFFTAGCGPSAKSDVKPGGAVTAANTGGSS
                     APVRVRSFSDSRPVKSLAAAAGLVWVGTPRGLVRWSTTSEPPTPAVLTTIDGLPADRI
                     EGIALDVKGGVWVTTSKGVGHFASGSWTKYPKAPVGDLVAGIVVTPDADNVWVGGSDG
                     LAKLNQGQWERYAAGTTVTALVHDGGSGVWIGTSGKGIMRVVKGDMLQFGMAEGNDID
                     NVRAMVADNNGSLLAVGDGPGGQRIAFYDGTRFWSYRVDSANIIEWVQRVGGELYLGA
                     GQSVWTMKRVATPAEVRGPLKFSFSGSPSMGAPKAQPLAGLLVKEAAPPPPPPAAAPP
                     PPPPEPAKGKGGKKDKGGKKTSALLVPSQDQYAAVAQPASAIRPDRPWLVAGPGGTAA
                     TAPLFDTDAVDVRLPDGITSVSADADSLYVGTRFLGVMRIQKGKQTPLRLFDLTAGAE
                     RLSVACINNNDCYVATGGTQSWRFDGQTFEVTNVDPEKGSHVLAVVRDPMGAVIALHR
                     GASSRSVRISRVGNKGDWSPVGLTPLEVPTGVPDLSFASFSPKGQLWVGLRYVDKEQD
                     ARAYGAAEVYVDDSRVVYHRQRPTGTQPGVSQGVNVPSDVTAIAWKGSTEAWLASGAG
                     AVRLQDNKTVKVFTENDGLESELVHDVIEGLNGQIWIATSRGIGVWDGNRWGFPKEPP
                     YNVKASALARDPDGRIWIGTDRGVIEVVAEKRTHQIGTRSGLLDDKILNMGVDVRGRV
                     WALTEKGISVIEPIQTN"
     gene            complement(11121..13622)
                     /locus_tag="PKL17_00350"
     CDS             complement(11121..13622)
                     /locus_tag="PKL17_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43200.1"
                     /translation="MGNQLPRDESPAFRFAADDLAQVMAKVAKVRWRLRLQAAARGVV
                     HGLIAAATVMLIALLLFRLELLSRFWLVRLALLCPVGVLLTAVGLSLRRIDPLWVAGI
                     IDKSHALHDRLSSAIQFAAGHTPLAPTDFAKGLAELAVHDAAQVAQKVQPSVAAPWKK
                     PPFLPLLGVLVALNGLLLFVRFERPKLPTEKAKSTPSAAMADTLHIDPELLGPEREEL
                     LQKLAEAEQRGDHETAQLLREMLDLLQQVERGELTRQQAFERLSEIEQRVRQGTEATL
                     AEMEKQLHRAGSELRESKLTQELGQALVKDDLDKAKAELKELADKALARAAAADKQQM
                     AEAFERAAKAMSSRPDENTPTNKQDGASQKDEWQAKNKSEEISQKLNELRDEKRKLEQ
                     QIEKNPNDSEARRKLDELKQAEQKLAEQLRQEQEKQKQELAQKQQQRNELEKNREELR
                     EEERRLKKKLAENPQDEETERRLKKTQRQLEQLEQKLNADGPGERQLQRLEGNQRPEY
                     QNEARKLLSEMEREQQKFEDEIRKLKEQKKQLEEDIRRLQEQLKKNPQDKAAQKDLAQ
                     KQQQLSQVEKQLREKQEAQKQMRELQRDLQQAAERMRQSLEKMTPDQRQAMEELSREI
                     SRYQDEVRKLAKQKAGQKQNIVTLDQIKQVLRRLGKSGQGQGQGQGQKDMQDFKKRAN
                     GDGDGQTLVLGGSGQDGQTTIVLMPGQASPGTGGPGQGQGGQGNQNGQGGPGQGKEGD
                     RPGTQHDPNFLGDVTQIESKRKLTRVYGKEGAGPSRSQTISGAAEKGFATASYRKVYG
                     DYTAVSEEVMSKQRVPPGYRFYVKRYFQMIKPRGN"
     gene            complement(13671..14252)
                     /locus_tag="PKL17_00355"
     CDS             complement(13671..14252)
                     /locus_tag="PKL17_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNK43201.1"
                     /translation="MIDALILSAGASRRMGTPKALCPLGDSVVIDRILSVLDELGIRT
                     PKVVLAKPHGDEIARWIKKSASPVDLVWNPAPEEGMLSSISCGLRALAPVEGVLLWPV
                     DVPLVQTATIKTLLAADRRRWLVPTFADRGGHPVWLPSEQFADVLALPSSTSLRALRD
                     THPPLRVPVDDPEVLVDVDTPAALETAQSRLRR"
     gene            complement(14249..15127)
                     /locus_tag="PKL17_00360"
     CDS             complement(14249..15127)
                     /locus_tag="PKL17_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNK43202.1"
                     /translation="MSREVEVFEALLASSRARESMVLCTVVRTAGSTPRKHATRMLIR
                     KVAGHIDQVGTIGGGRVERQVLLRAAELLALGQKAQCERVRYHLTHELAMCCGGEMEI
                     LLEPMFPDELVIVCGGGHVAKALVPLLRPLDFVPIVVEDLPELGNPERFPDTQIIDSF
                     DVRDWKDIPLDKRTYVVVVTREHATDQAILEQLLGKPLAYLGMIGSQRKVTTFQKRLL
                     HKGFPEAEIAQIHAPIGLPIGAETPAEIALAIAAELTAVRSARRNHAAKPAAQPHSET
                     ARTAAPRLLAEASKLA"
     gene            complement(15195..16607)
                     /locus_tag="PKL17_00365"
     CDS             complement(15195..16607)
                     /locus_tag="PKL17_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNK43203.1"
                     /translation="MAQLYSQAREVLLKLVYYGPAGAGKTTALTQLHKLLRPEVRGQI
                     VSVNTGADRTLFFDFYPPPPPLLGAPVHAQVFAGSGSVQNESTRRALLAGCDGILFVW
                     DSRRGRENECAAALEELRNCLLDLGMRLQDVPLVVAWNKRDLADAVALSDLEPAFGIR
                     QSQSVPMIAATGQGVFDSFRMLATEALASALRKRPELGQTGSMPTSDLSFGNDMVTAR
                     RMLAAHEAQAALRSLVQRYTLDQPLRIDESALLREQAGRSQEILPMSGLSAPPLPNPD
                     QSSEVVQAVRADGQQLPAVEEIIRPRASRPPRPIAVDTPPQAAPPRPHGAAALDKTMP
                     GSRLAQPSKPPLQPQPPRMEAAGRDQGLSMLLAPGPQRAQVQEVERLIHATQYVQAVR
                     RTVGIFFSLQVSDTAREPDEGPAWRALVLGLPADRYLRFRQAGHSAESSRSTIEDAYF
                     ALFFLVDAMMRKEALGPRSS"
     gene            complement(16718..17332)
                     /locus_tag="PKL17_00370"
     CDS             complement(16718..17332)
                     /locus_tag="PKL17_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43204.1"
                     /translation="MSSHRGRRPLGWLSALGLGLALLGTSACGDTDGLTVPAEAQASC
                     QGEDPTDAMKMATATMLPGRVCGACHSTGGQAKNSPWTVSGTLFANKTSGCNSGEGLP
                     NMVVEILYGQDDPQGAYKYGQVQPKGRLTTNSVGNFYTATKFTAPMIARVCETTGPDC
                     STAKRVQTMIQKVGQDPATGATVRVDCNLCHYAGGQALTRIYLP"
     gene            complement(17355..17864)
                     /locus_tag="PKL17_00375"
     CDS             complement(17355..17864)
                     /locus_tag="PKL17_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43205.1"
                     /translation="MNLRTIACALLFAMPLFGVGCGDTADLNAAPQAKAGCEEPTAEM
                     LKASDAMLPGRNCMTCHAAGQQAADKILGTGGTVYGAKNSKTCNTGGVADVKVDILDT
                     AGNVIASTMTNSVGNFHFPTNAAFKNATARVSKGAMLLKMTGAVDLTTGCASCHFASG
                     LAADRIYIQ"
     gene            18133..19407
                     /locus_tag="PKL17_00380"
     CDS             18133..19407
                     /locus_tag="PKL17_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
                     /protein_id="HNK43206.1"
                     /translation="MTNDQTSTPSSSSSPGTASHRSIGIDAIAFAVPRAYVELADLAA
                     ARGVPPTKYIQGLGVVQMAVAHPDEDPVTLATEAARRCLMAAGRSPAEIGLCIVGTET
                     AVDHSKPVSSYLHGLLGLPSACRVFETKHACFGGTAGLLNAVDWLAAGSARGRCALIV
                     CTDIARYSVGTAGEPTQGAGAVAMLISENPRLLELEPGITGCFSQNVHDFWRPLYRKD
                     ALVDGQFSVQCYLDALAGAYAEWQRLSESSEPLVRTCYHVPYGKMARKAHQRRCQLDG
                     MTDEAADKSFAVEVEPSLRVPSQVGNIYTGSLYLALLSLLYHQAAELSGKRIGLFSYG
                     SGCAAEFFAGRVMPTAAAQIARAGVDTTLANRHRLDIPVYEELRKNDIHVDQRPSEMP
                     SAAETGAALAMVDRSPRFNGIDNQERRIYTVA"
     gene            19548..19621
                     /locus_tag="PKL17_00385"
     tRNA            19548..19621
                     /locus_tag="PKL17_00385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19582..19584,aa:Ile,seq:gat)
     gene            complement(19626..21197)
                     /locus_tag="PKL17_00390"
     CDS             complement(19626..21197)
                     /locus_tag="PKL17_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43207.1"
                     /translation="MTETAAHNVAQNFDPPPTVLGKYCPSCHSVYLPPDGDANFCAKD
                     GAQLLGDQRVFGGKYVLGEPLGYGHMSEVYVATQAGIGRRVALKLLRSEHRQSAEMRK
                     RFEREAQIASSLDHPNAVTIFDSGHTSDGQSYIAMELLGGETLDRLIQREAPMPLLRA
                     LELLIPAVRALAAAHTRQIIHRDVKPDNIFVARKPLSVGAEEVVKVLDFGIARVLGRK
                     TRVTSTQTALGTPHYMAPETLQGDEASPQSDVFAVGVILTEMLTKKLPWGETSRSEVS
                     MVMARMVSPPLRLTELLPGTTFPPDLQPALDQFLSQSLENRPVDCAVALQILIQFLPS
                     TSGQTIRPALPQPAASPGEVSTGSGPPTLAPGDSGAPPTVISSSGATRPLRMATAVLT
                     NGAHQTAARRARTLAVASLVVAILTVLWGVWLRKRPPPTPSFASQQTAQREQAAQKVL
                     FVPEARPGSEPAPVTASVASAEPADAVAKKAPSAEPETGTEETSKATKTRRHRHRAKP
                     APPKGPSFPLDTKGY"
     gene            21612..22259
                     /locus_tag="PKL17_00395"
     CDS             21612..22259
                     /locus_tag="PKL17_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNK43208.1"
                     /translation="MSEELLTPSARTPSRLGMSRRPLRGRPVPSRSRSHLLEAEMVEI
                     EQQHPDGMTLTQVLSAFGDRGIHLSEATFRKYVQLGLLGRSRRVGRKGKHQGSLGMYP
                     ATTVRRLNTIRQMLGAHYTIEDIQRSFLRFADEIDSLQRGLAGLLDGLSRELDGGQFA
                     ADRKRAMTKELLAIRKLGSDVVQRIEALERELVSPLERAARQRTFGTSSGSDELL"
     gene            22303..22806
                     /locus_tag="PKL17_00400"
     CDS             22303..22806
                     /locus_tag="PKL17_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNK43209.1"
                     /translation="MQDITQPPDATPSECNETDGNTTPEVSRKVRKHHKRSRSKTIAP
                     RRLTKEELREAALLPVIDDSERPQTRADCAGGARPCPWVSCKHHLFLDVNPETGSIKL
                     NFPDKELTEMRETCALDVADRGGITLEEVGAILNLTRERIRQVEVRGLLKLRAVSPDG
                     DVGRDMP"
     gene            complement(23013..24635)
                     /locus_tag="PKL17_00405"
     CDS             complement(23013..24635)
                     /locus_tag="PKL17_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43210.1"
                     /translation="MGRALLVGLTGFGALSLHPSVVAAQEKKSVVVLPIEGAPPKLQK
                     AIENALKKDYTVVPEAKWNAAAKKLNVTGHGTEDVALIANDQKIDVVITGKLKTDKDS
                     GQYKLNIAARHGASGKPVGKLTYDLKNDKVEPATITTVESEIGPAVVAAIAGPPEDRP
                     APVAQAEPPPSDPTSKLGKEEDPIAKLARDEAEKRRKERELLRPIYYPFIDAGAGVIV
                     GGRSFSYTEDSSPDSPLKCYDLEKRVPDPKMPLGRGYVSHYLPYKRGTTAACPGFATS
                     IATGVRVDLTAYPLAMMRIGAIKGLGIGATFDYFFWPDSKYGSGTSQVLLATREWKVE
                     GGLRYHINLLNKRNMPSILFNAQYGAHFFAVAKEQKQYPYQDENFIDKTADGVNDHGL
                     PDILYQYFTLGLGGRFPYFVNDKVYFAGVVDVNVHIPISYGEIGQRFGTANTDTASGP
                     VADLYGNGGFGPASGYGLRASLTLLEAMVWKGLTIRLSGYYELFNYTFDLGKTLVDQT
                     PGDVATGRDARHLAAGASDTYFGGVVQIGYQY"
     gene            24978..25943
                     /locus_tag="PKL17_00410"
     CDS             24978..25943
                     /locus_tag="PKL17_00410"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK43211.1"
                     /translation="MQTSPVGTVLPSAVLSALSAASSDGQRTVSARAASGSSGAIGDL
                     AQSGETVKTIRDSSDSGALGRSVPRVVADEPTLPVRYRPTRKRSAARALRSVLFGLLW
                     LLLAGLVAMSVVWLLPERQAGPAKETPATVIASVKEPSKPPRMTARPAVPAVPKAATP
                     SPVAAPGSESPRVIPAPDSVPKAPAVPSPVPAEAVETVHSPSTTPTSTDFSAVLSGAA
                     RAAWQQDLANIEFVATTYAAQVRACYERSIRGLGGEAPSGRVVVLFSLTDDGKAQQPV
                     ISEDSLGLPVLGPCLTQRLSEWRFPRPVGPIRTFRFPFVFAGSRP"
     gene            26060..27127
                     /locus_tag="PKL17_00415"
     CDS             26060..27127
                     /locus_tag="PKL17_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43212.1"
                     /translation="MSGKTGDEKQSSTLVASALVISQREQVQAELQRLLAEADVNAVL
                     VSDSDSARAQVAKTQYPVVLIDVDSISLSQAVVLLGDVRRLAPRAELIVLLDEPNFDA
                     AVQLVRRGAVDVVCRSSDDLRLLGPRVASAMRDAKQRADRSQMLRDYSILTEDLLYRL
                     TDTARRIGELKAQIHSSAKGSGSPASPIDDYVSVLIVEEDGWLSQSLSPLVSSSFSLT
                     TVVSGGAALDRVSERPFDLALIKETLPDLPSRTVIRSLQAQSPETLVMLYLPPTRGKP
                     GRIDRLEAGGGKVTTLVSAFTSPKQLAERLSELQQAQAARRRERRYLAEFRQENYELL
                     RRLSELRKRYREVDSTQGRGK"
     gene            27263..29023
                     /locus_tag="PKL17_00420"
     CDS             27263..29023
                     /locus_tag="PKL17_00420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43213.1"
                     /translation="MNDVPTTGVTRVCVTCQQVYNYKDQHVCPKGGKAEVPPDPLVGA
                     TIGGRYLIESFLSSGGMGVVYRAKHTVLDKPLAIKLLREAQDPMMQQRFLLEAKSACH
                     IGHENIVDITDFGVLHDGRPYLVMEFLQGQSLEAVIAKGALPPLRTCRIAEQIARGLH
                     AVHSKGIVHRDLKPGNIFILERGRKDFVKILDFGIAKVMAGTRESLSGGSANLPNLRA
                     TATGTVLGTPEYLSPEQGAGEAFDARVDQYALGCIMYEMLTGQVPFRGNGPMSTLMKH
                     LTEKPIPPRKRRPELNISETLEKIVLKAMAQQKESRYANMDELANVLQELIDSGTLPA
                     DPVVPRPSASLQTVAASLSASSAPPVELSPLTSGDMFAPTLQAAIPTKDEIAKERAKT
                     QPESTTATIDRGNKPPSENREKAPLATEQVRPIVLAKPNRLPLLLLGVAAVMVLLLIG
                     VLVVRLGPGSKPSQPVPAVDLGERKVETPVPVESPPTDEHKVELENKTAGKVTVLCGP
                     KKSCILDAGKTCELALSPDARCEASATGFVSRSLDVGSAVKHAGKGQTAHLAVHLDAE
                     KPVGKVKKSKGSKAGKGKKR"
     gene            29098..30792
                     /locus_tag="PKL17_00425"
     CDS             29098..30792
                     /locus_tag="PKL17_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNK43214.1"
                     /translation="METAAKTEPTHPVPASFETSPDRYRHWRLEFDGDVARLLMSVRE
                     DEPLVPGYVLKLNSYDLGVDIELCDAVQRLRFGHPEVRTVVVSSGRDRIFCAGANIHM
                     LAQSSHGFKVNFCKFTNETRLSIEDACASSGQKYLAAVSGPCAGGGYELAMACDEIVL
                     IDDGSSTVALPEVPLLGVLPGTGGLTRLVDKRKVRRDRADIFSTLAEGIRGKRAVEWR
                     LVDQTVPRSRFAQAVSDRAHKLAQESKNQVVSAPGPQTGISLEPLHPTVDGDSISYRF
                     VSLRVDKPHRTAHLTMRGPGAGQDVRTAEELHALASRGELWALRAYRELDDAICRLRF
                     HYPTVGLVMLSTRGDQAEVLKADAAVAALRDYWLGREVLLHMARVLRRLDMTAKSFFA
                     VVDPESCFAGSLLEILLASDRSYVLEDPGVKFSTGTLNGGALLCGNGQTRLSLRCYDQ
                     PGRAKEILARGEKGPISASEADALGLCTVLADDLDFEDTLRLAVEERVSLSPDALTGM
                     EQNLRFPGPDLSEGKVFGRLSAWQNWIFIRENATGPLGALTCYGRPERPSFDWRRT"
     gene            30974..32425
                     /gene="boxB"
                     /locus_tag="PKL17_00430"
     CDS             30974..32425
                     /gene="boxB"
                     /locus_tag="PKL17_00430"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HNK43215.1"
                     /translation="MTVELFEKIPNNVNLSADKRLQRALEQWLPNYISWWKEMGPDQF
                     QESDVYLRTAISVETDGWAHFDYVRMPEYRWGIFLEPAIAGRTIGFGDHYGQPAWQEV
                     PGEYRNTLRRIIVTQGDTEPASVEQQRMLGHTAPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHSYFGKDGREEAEAMLERRSGDRDKPRILGAFNEPVKSWLDFYMFTMFTDRDGKFQ
                     LCALSESGFDPLSRTCKFMLTEEAHHMFVGETGVDRVVQRACELMLETRSDSPDVITK
                     AGGIPLDMVQRHLNLWFSLSEDLFGGEISSNAAEFFAAGLKGRYKEDSYSEHTGLNQF
                     YEMPVPAEGGKIVTTQVPLRNAMNEVLRDGYVTDCQRAVDKWNRTIDKQLGERGAQFK
                     LALPSRRFHRHIGIYGMNSGNYFDPTGNPISQGEFMANRNKWLPTDADREYLKQIMRP
                     VTEPGKIAAWIAPPARGINGQTVEFEYVRHLPA"
     gene            complement(32450..33688)
                     /locus_tag="PKL17_00435"
     CDS             complement(32450..33688)
                     /locus_tag="PKL17_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43216.1"
                     /translation="MNRALIPIFLIVLVDVFGLTLVLPLQAIYAEHLGATPLQATLLV
                     SVFAICQLVASPLLGSWSDRIGRKSVLLFSQIGALFGYLAMASATSLWMIYLARVIQG
                     TTAGNLSIAQAYIADHSEAKDRAKSFAVIGVAFGLGFFIGPGVTGVLVKHGLAAPIFL
                     AAGMSLVSIVCTLLLLPGGVAIRAQQPDAALPGGKRLSILEWRSYRAYFDRPVLAAML
                     FQFLFYSMCFSVFTSGFSLYAERRYVWHGHPFGPREIGYLFAYSGFLGLLLQGGLIGR
                     LVKRFGERSLVNAGYLSLVMGFGIIAASNSLQWLGVAATVTAFGNAVLRPTLSSLISQ
                     SADRSEQGTVIGLTQSLQSVAQIVAPFLAGLLLERNLLWQWGAMASVAALGGLLIAPL
                     GSQRAHSAAQELASDNKPAG"
     gene            complement(33748..34812)
                     /locus_tag="PKL17_00440"
     CDS             complement(33748..34812)
                     /locus_tag="PKL17_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43217.1"
                     /translation="MSCTLVQVGRFTLGASMTFAGVAQSVTEREDQAVALTPLLEVAA
                     SLRRARDGELPTGEHKQRLLHLVPRLGSVLLPQLLRSLASNSDSEAQWAMQLLARADV
                     TDVTTRLRKLLCQSTQSDTVKARALSVMSDLGVSPPVDVAIQDPDGFIARSVAQLLAT
                     IACADDLRRTVDDLLGSVPPDELASVMREVVRHGGEIGTALLEMVMLDARTPHAVAVE
                     LIPLTRPTREGALLGKTAQNPLPKPHAVRRSGKAKMPRPSSPRSLLDALPVRRRLATS
                     EGRAKPTHHDKLLPAVTALSAAQFDVAVELLTEVVTQQPEHHVSWLHLAVAHSALAQH
                     RQAARCMRRAILLQSSRPHA"
     gene            34928..36253
                     /locus_tag="PKL17_00445"
     CDS             34928..36253
                     /locus_tag="PKL17_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43218.1"
                     /translation="MMAESFHKKALARELRPALSKDEALTEANRCLFCYDAPCMRACP
                     THIDVPLFIRQISTANPTGAARTILAANILGASCARVCPTEVLCEGACVLSDQHKPIQ
                     IGPLQRYATDHAMQTGMPILKPGKKQPGSVGIIGAGPSGLACAAELLKLGYESVIYEA
                     HDRPGGLNSVGVAQYKMTPDVALAEVDWLVRSGVQIRCGVTVGKDVSFAELESRHDAM
                     FIGVGMGGIPGIGVAGEDISGVWDGLDFIAALKQGDSEVFDALRGARVAVIGGGNTAI
                     DVVTQAARAGASKVWLLYRRGREQMSAYAHEIELAKQHGIELLLHAVPHRVLGPSRGL
                     SPRSSRVAGLEFGRKLPDGGERLETLPADLVIRATGQKGAKLTAELPVRTDGGKVLAD
                     ESGRTSNPRYFAGGDCVSGGQEVVNAVAEGQRAARTIVAEVLSLRGGAR"
     gene            36271..37557
                     /gene="preA"
                     /locus_tag="PKL17_00450"
     CDS             36271..37557
                     /gene="preA"
                     /locus_tag="PKL17_00450"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HNK43219.1"
                     /translation="MADLSVDFCGIKSPNPFWLASAPPTNTGDQIMRAFDAGWGGAVW
                     KTLGQPIVNVSSRYSSVDYAGRRMMGLNNIELITDRPLDVNFREVSEVKKRYPKNALI
                     VSLMVESKREAWHEIVRRAEDAGADGLELNFGCPHGMSERGMGSAVGQVPDYTCQIVK
                     WVKEVAKTPVIVKLTPNITDITFPARAAEQGGADAVSLINTINSVTGIDLETMTPRPA
                     VHGASAHGGYCGPAVKPIALSMVASVGQTVKIPISGIGGIANWHDAAEFILMGATTVQ
                     VCTAVMHHGFRIVLDMIDGLSSYLDRKGLASVSDLVGRSLPTYQRWEQLDLNYRVIAD
                     IKQDKCIGCGVCYAACYDGAHQAIAAKPDGRGRSAVQVLEHKCVGCNLCSLVCPVEDC
                     ITMKRVDTGSDTRVPWTEYSKNPSLYPHIRSNRHDQ"
     gene            37561..39051
                     /locus_tag="PKL17_00455"
     CDS             37561..39051
                     /locus_tag="PKL17_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK43220.1"
                     /translation="MLPSLVTVAQGLYGLLLLGLWLLLIAASAHLLRLGLWALAHPLA
                     SVSVRKLADGDLPVVTVQLPMRNERLVAARAIRAACALDWPKDRLQIQVLDDSDATDE
                     TVAIVDAEVAALRYAGFDISAVRRPDRRSFKAGHLDFALPSSRGEFIAVLDVDFVPQA
                     DFLRQLVPHLVAQPQLAFVQGRWSFLNEDDSLLLRLQALILHGLFLVEQSYLSAHRQP
                     VQFNGSGGIWRKAALSQAGGWVGGDSDTEASVTEDLDLSFRVRLFGYESLLLPQVAVP
                     TELPESMAAFRSQQKRWVRGGAQVLRSLLHKLWSPGRSLGERLTMLLHLLRHARQPYL
                     ALALLFLPLPPLGVLHGLFAPPGGMLAAVLILWTALSVYYGAALLRLQRSPFWAVLLA
                     PLVMALSMGLCVSLTVELLRGVLSSRTGAEFVRTPKTGGQKEPAKQYRPVFDRLARIE
                     VGIGGAYLALSFWLFWRGDYLTALGFGLLIGAGLLWVGGGSWRISR"
     gene            complement(39055..39840)
                     /locus_tag="PKL17_00460"
     CDS             complement(39055..39840)
                     /locus_tag="PKL17_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43221.1"
                     /translation="MLGIFFLLVWVTVIAALTSHERNRSALLLSEHADDDPPSLPSDD
                     PALLIRYYRVALARTMPLREEQLLRINLACALNAVSDHREALEELDKVQLQQLSPVEV
                     ALWLNNRAYTLTFLGKPDDAVGHLDDAVELLAGDDGPSKDPLLSACISGTRGIAELHR
                     KNYQASEAALLSALRSEEESVSLQFDPEWQVDPGRTAERWFWLGEVAQAENKPVEARR
                     RYERAAAYPQTPFGKRAKVALGHAASALPDPGAAPAAPSASSA"
     gene            complement(39886..40224)
                     /gene="cyaY"
                     /locus_tag="PKL17_00465"
     CDS             complement(39886..40224)
                     /gene="cyaY"
                     /locus_tag="PKL17_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HNK43222.1"
                     /translation="MNPREKQDAEFDKLADAMIRRIERAVSTFDPDEVDVEMSGDVLN
                     LTLGNGQKIVINRHRAARQIWMAAQRKAWHFDPDADGKLWKTADAELVSTLETVLSAL
                     LARSIRLGIS"
     gene            complement(40307..42805)
                     /gene="pheT"
                     /locus_tag="PKL17_00470"
     CDS             complement(40307..42805)
                     /gene="pheT"
                     /locus_tag="PKL17_00470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNK43223.1"
                     /translation="MKVSWNWLCELLEIPSSVTPQAVADRLSVSGVAVDALHPIGKGL
                     SGVICAEVRGKRPHPKADKLTLVDVFDGKEVTQVVCGAPNVPNPGEPGRSPRVLWAKP
                     GATLPNGLVLSVREVRGIPSPGMLCAEDELGLSEDHDGIVILSPDDGIDIGGDVAQAA
                     GLPDWVFELDITPNRPDLLGHTGVAREIAALFRHEGVRLRQQSVNLAALRGNQAQTEP
                     AQIVVEDSESCPRYLGHLIRGVKVGKSPLFYRLRLQRLGVRALSNVVDATNLALLQYG
                     HPLHAFDLHKLAGQKIVVRRAKPEEKIKTLDGQEREVGPSDLLIADAEKGVAVAGVMG
                     GQTSEVGESTTDVLLECAYFAPAMIRRTAKKLKLHTEASHRFERGTDANAIPEVADAC
                     AALIVKLAGGQLAAAATDVYPKKLSLPTLTLRPERTTAMLGLTISAAMQKECLEAIGL
                     PVHSDGKLLTVEIPTRRPDLTREIDLIEEVARVYGLHHIPTTLPNMPMSEPQPPSVQM
                     TRYRNADRARMICASLGLLEVIQFSMTSPDRVKLGEGRRELAPLCIENPLREELSVLR
                     SKLLPGLVELVRQNLSHGQTDLRLFEVGEVFQPSGANEVLPTEQTHVAAVLCGHRDHF
                     LKPTPGDALDFYDAKGLVEQLVAGLGLSLVWQPDPSAGARAVFVRAATADEAPMLHPG
                     AAAALCFAHSGAVIGTFGELHPDLRSKLDLPGAAFALELAVPDLPISKPNYVAHSRFP
                     ATSRDLSFFVAQDVSAAALLDAMQKSEEPLLRDVALLEDYRAQGHVPAGQKALLFSLT
                     YQSDQRTLTDDEVQKAHQKVIDQLGKKVPIALRQ"
     gene            complement(42821..43879)
                     /gene="pheS"
                     /locus_tag="PKL17_00475"
     CDS             complement(42821..43879)
                     /gene="pheS"
                     /locus_tag="PKL17_00475"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNK43224.1"
                     /translation="MALAKDEPALRVVYARFAGKEGEIRKRLADALKAAPGPEKRAEQ
                     NPNFPRGAAPTNHPYTHPKAEIGRFGNVALQKAEELFAARLAELHEQKRQADLQRRLD
                     VTIPGRQHKLGGLHLLTQVRLELEGIFAELGFHVATGPQVETDFHNFEALAMPKDHPA
                     RDMQDTFYLQSKPGHEPLVLRTHTSPVQIRTMLAQKPPVRIISPGKVYRKDDDPTHSP
                     MFQQIEGLCVDEGITMADLKGTLLYFVQRFFGPGIGLRLRPSFFPFTEPSAEVDIECF
                     FCKRSGCRTCKGSGYIEILGCGMVDPEVFRHVGYDSEKYSGFAFGTGLERMTMLRYGI
                     SDLRLFFAGDARFTEQFR"
     gene            complement(44034..44393)
                     /gene="rplT"
                     /locus_tag="PKL17_00480"
     CDS             complement(44034..44393)
                     /gene="rplT"
                     /locus_tag="PKL17_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNK43225.1"
                     /translation="MARAKRGFKARRRRNRIKAFAQGYRGGRSTLWKAMVETVRHAWR
                     FATWHRRERKRDFRRLWIIRINAAAREIGTTYSKLIHALAQANIKIDRKILSDLAISD
                     PAGFKAIAAQAGIKAQA"
     gene            complement(44578..45117)
                     /locus_tag="PKL17_00485"
     CDS             complement(44578..45117)
                     /locus_tag="PKL17_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43226.1"
                     /translation="MAGKKTGTAFDITALFEQELQARNEDLRRQAAMTMFFKLHPTNK
                     TTVEEFITSLKQHKDVWAAVSTMGIIDFVEAIGGKRPTESKPGKAAAKRTRLSEAQKN
                     SLKGIITSVLSNAKDGLSRGEIADNINNELLANVGVDRAELANKLRQPLGELVADNKI
                     HTIGEKRLMKYHNGPGKKK"
     gene            45331..46944
                     /locus_tag="PKL17_00490"
     CDS             45331..46944
                     /locus_tag="PKL17_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK43227.1"
                     /translation="MKIRRQLSPLYLSFPLVLIAALALARYSYRNARQLAQNSEGSVI
                     AGLFALGDQTRSRVESFIIDSDRELFNLVDLEHLQDFSRRWSEIVRLSHAVEAAIVLD
                     ENQQLVRGGYVNKRRNKPEAEAFLRLFMDRIRPELPLAEQRPDFHRHLHTNVDGVDYL
                     LSYIKRYDAARNRVFYIILKVNLDYLVGTSFPEMFEPIAGRFLVSVIGSRGEVIFGQP
                     ISGAGKYLYDKRFESTVYQWRLQMAPLDNVKLRAGSQKQALADTILIGLMLGLILIGT
                     AFLVYSISTEQRLSALKSEFISNVSHELKTPLSLIRMFAELLMLGKVKTPEKGREYAG
                     IITRESERLSRLIDNVLDFSRMERGKAAYEMKPGDLGEVVERALDFFRYRLEREGRQL
                     EVDIAESIPRTLLDENAMTLLLLNLVDNAVKYGGTGPIAVRLCVSPRGKEILLSVTDH
                     GLGIAPDEQRKIFERFYRTRAVRNTNIRGSGIGLALVKHIAEAHGGHITVDSQGGKGS
                     TFTLTLPVSAIKEEPDEAGGDAAFDKGDS"
     gene            46945..47703
                     /locus_tag="PKL17_00495"
     CDS             46945..47703
                     /locus_tag="PKL17_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK43228.1"
                     /translation="MALPSSGPASASSPPQSGSGELSKRARILLVEDEPDIVRGLTDV
                     LEFEGYEVVTHMQGRAGVREMKERGADLVILDLMLPDINGFQVCEEIRAENQVVPIII
                     LTARGQELDKIRGLEAGADDYVTKPFGVGELLARIKAILRRLTRAQVSKEPETIRIGS
                     CEINLRTHTLVRARKSHMLTFYEVELLKLLYEREGQPVSRDEILDKIWGIEAYPSNRT
                     VDNFVVKLRKKLEENQHHPRHILTVYGFGYKLVP"
     gene            complement(47700..49442)
                     /locus_tag="PKL17_00500"
     CDS             complement(47700..49442)
                     /locus_tag="PKL17_00500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43229.1"
                     /translation="MENLRREPGDGTPQRESATIESTPHQLVDLSAKTIDRQADRRTA
                     ATPVESPPISRAETAIKAPGSSTGAQAIVPIVVNPQLLDPVRIGRYMVVRCLGQGGMG
                     TVYAAYDPDLDRKVAVKVVRDDVHRGQIARERLLKEAKAMARIAHPNVVHVYEVGEDR
                     ESPQGQIFIAMEFVPGSDLVEWQNQHPVRDATSLDHCLRTYLQAAAGLSAAHNSGLIH
                     RDFKPDNVIVGSDGRARVMDFGIARAPDDSHSHKNESPEASQAKLRTTSERLTQFGAI
                     LGTPGYMAPEQVRGEQADARSDQFGFCAALFEAIYGFPAFAGTTLEEYAASIRSGKPS
                     TRTKTLHGYEVPLVIHAALMRGLSIDPMERFPSMQELTEALQAGLLPDADSESMRQNK
                     RRFLHVMLSSFSVIVLGVLLALGGVHRSDLRPALLIATALLSVTSLSVAGFRQQIHQQ
                     PGYRRLGYFMLATMSYLVFGRSIGVWLDIPADRFLIQETVGLGALFASEMPHVGRRYG
                     WIVALCLFSVLLQVVWPAGRRLHLNLTYGVMLFLTIYLRFDRGTLSHRSTPQFPEGTR
                     RDSPSWNEGRPSRG"
     gene            complement(49777..49983)
                     /locus_tag="PKL17_00505"
     CDS             complement(49777..49983)
                     /locus_tag="PKL17_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43230.1"
                     /translation="MSFKQLLVGILVGVTSCLLLPFAPASASDKEDVKSDKVARADKD
                     KVQKPVRTPSPWDTRPMVPAGPKY"
     gene            50265..51329
                     /gene="dusB"
                     /locus_tag="PKL17_00510"
     CDS             50265..51329
                     /gene="dusB"
                     /locus_tag="PKL17_00510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNK43231.1"
                     /translation="MRIANLVVDTEVLLAPMAAVTDLPFRTICEEHGVGLTITEFLSA
                     RALCEGAKKTTQKLTASLGGRRFGVQIFGREPDALAKAAQLAKEIGASLVDINMGCPA
                     KRVVAGACGSALMKEPELAQVLVRAVRSALPADIPVTVKHRAGWDEKHLNAPEFACAM
                     VEAGAAMITVHGRTRTQGFSGKSSLDIIRKVRDAVPRHVPVVGNGDVITLLDYVRMRT
                     ETGCDAVMIGRGALGNPWLFRDIGAWLRGQPVPGAPTVEERSEVFLRHVALMRGRTED
                     KRLIHEVRKAVAWYTKGLPGSAELRDQHKAPREPDALVAATVAYFTELAKGRPRLKVA
                     LDASAAQAGTDETTAKAVCA"
     gene            51326..52354
                     /locus_tag="PKL17_00515"
     CDS             51326..52354
                     /locus_tag="PKL17_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK43232.1"
                     /translation="MKAASLAPCIALLGEAPVTLRLRALVEQAAQVETPLFVEGERGS
                     GREHIGRYIHSTSPRRSAAFIRIDAEYADTERAIGKLRRSHGGTLLVKDVPYGPLRLR
                     KVLQGLLERQELESGQLRSEGEGAELPRLILTSDVELPRLRESGFALAELLLLHQVTR
                     LRIPPLRERVSDVPLLAEHFLQLLGEQMGRRHKLCSRAVDQLIRYSWPGNLAELRDVI
                     SRSALRSRGETIELGEVEAVLPPLQERVPIEQLSLEETVRAKLRALLSRLEGCPLHDL
                     NLYEEVLGHVERPLLKEVLQRTEGNQVRAAAILGINRNTLRKKLRERGLGSEFLDTPT
                     DSPLRHLP"
     gene            52429..53361
                     /gene="grxD"
                     /locus_tag="PKL17_00520"
     CDS             52429..53361
                     /gene="grxD"
                     /locus_tag="PKL17_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNK43233.1"
                     /translation="MALSPDTRSQIETLLRQHTIVLFMKGSRQMPRCGFSAKVIEILE
                     GHGIDYRDVDVLSDPALREGIKEFSNWPTIPQLFFQGSLIGGSDIVRQLDESGELLQA
                     LGIDPSKVSIEPPRISITPEAAAAIKDAGKEAEPGQHLRMVLSRGGQNIDLYFDAAKA
                     SDLRVEQHGITVLFDRASARLASGISIAYVDGPGGGFRIDNPNLPAQVGTLTPAELRK
                     MMDAKVPLRLIDVRTERERAIARILPDVTLDQIAPELDSLPKDTMLVFYCHSGVRSRA
                     AAEQALARGFRNVWNLNGGIDGWSRQVDKDVPRY"
     gene            53377..54267
                     /gene="rfbA"
                     /locus_tag="PKL17_00525"
     CDS             53377..54267
                     /gene="rfbA"
                     /locus_tag="PKL17_00525"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNK43234.1"
                     /translation="MKGIILAGGSGTRLYPLTKTLSKQLLPVYDKPTIYYPLSTLLSS
                     GIREILIISTPRDLPMIRALLGDGHELGISLSYAEQPRPEGIAQAFVIGADFIGQSPV
                     CLILGDNIFFGHNLVDDLKRSAGLTTGAEVYAYHVHDPERYGVIEFDATGKAISIEEK
                     PRAPRSNWAVTGLYFYDASVVDIAQRLTPSARGELEITDVNRAYLKKGELRAHKLGRG
                     VAWLDSGTYDSLLQASQFVQTIEQRQALKIACLEEIAYLQGFIDAAQLEKLADSYSKS
                     SYGAYLRHVLSENRGQASAS"
     gene            54370..55161
                     /locus_tag="PKL17_00530"
     CDS             54370..55161
                     /locus_tag="PKL17_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK43235.1"
                     /translation="MPSDTTTRVGANTQFDYEGIPAGYYDRVMREGNPIRRLWHVSKF
                     ERVLDYLPQPSDPHAESSILDIGCFAGTFLSLVPQDRFSRQLGVDILPAQIDYANRTY
                     GTPFRSFRHVPSITHLRDIEGHFDCVTLIEVIEHLTAAEIHALFSELARLIRPGGKLV
                     LTTPNYLSCWPLLEVILNRMSEVSYEEQHITRFTYFNMERRLCELYPSFAAEFALDLK
                     TTTHLVTPFLAGLSYDLARNLSRMVPHTSWRMPFGNLVLMVLTRR"
     gene            complement(55186..55710)
                     /locus_tag="PKL17_00535"
     CDS             complement(55186..55710)
                     /locus_tag="PKL17_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43236.1"
                     /translation="MAVQKPLYDQEMLLPLALLPLACISWNQLQDTLPPEAVVHVCPG
                     ERIRMVRVSQITTPSGKKPYRAVLVVRQDDTVAVKTTRHQGEFRLSAEQKNDLRDALD
                     VIRDYRKHMFHRLPRRVHPSDDGGIDFYIEIQDGKHSLRWSNTQYERPPAVPSLFDVL
                     EVYQYLLSPKSDDE"
     gene            55897..59832
                     /locus_tag="PKL17_00540"
     CDS             55897..59832
                     /locus_tag="PKL17_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNK43237.1"
                     /translation="MASKWVGSRIFLGAVCSVLGACGMPSAESPTEPLVQPQKPIAVS
                     VRPLHNKQGAPSLSSVATATKVVSDSPEQVAWATLRGIKDGHKLSGEAVESAKLREVH
                     DAGNGPLIARFEQEVDGLPVFLQSMNIAMDRNMQPTMASGRLATKLRKVAERFQLDES
                     VAVESALRAMSGERGSAVSMGAAQAAPGGYQNRQFVAQLSGSGAQASVSSRSRKIYFP
                     TDKGLVPSYYVELDVAPLDSTSSTMRAFVVSSIDGKVLFEKSLTEDVAYSYRVYSDGA
                     PGYVPWDGAQGNDYTPYPKAAPDGSAPALGVSSLVTLENVPFRRNDPWLPPGATELSG
                     NNGWAYVDQIAPDGYSAGDLLVTPTSAGSFDRTFDLSTTAPSANDTAKRTVATNFFYI
                     VNYLHDVTYDAGWTEAAANPQKSNFGRGGVEGDMIRLEAQDNSGRNNANASTPADGGM
                     PRIQMYLWDASSTSLTVTAPAGVAGTYQSGGASFGAKSYNITNSVIPATDAPGPAGST
                     TDGCDAITNPAAIAGKIALVDRGNCNFSVKAVNVQNAGAVGLLIANTMAGATAFGPGG
                     TDPTVTIGVQGISYADGVKLRTAAMSGTVTAKMTASYNERDSSLDGSIVSHEWGHILS
                     NRLIGNGSGLGNNQGRSMGEGWSDFNAMLMTVRPEDAAVASNNNWSGTYSTGAPSVFS
                     PGNNAFYEGIRRYPYSTEKTKNPLTFKHIQEGVALPATPVPTFGASGVGNSEVHSSGE
                     VWANALWECYAALLRDNTRLSFSQAQERMRRYLVASLKLTPNNPTFTEARDAMLFAAF
                     SSDRKDYDLFVDAFAKRGMGSGAVSPDSASTDHAGVVESFATGAALYIDKDSLAVDQS
                     LMACDTDGVLDNGESGYLSFRIYNTGSKDVQNGTVTITTPTGSSVTVNGQPTATLSLP
                     PIPPFSSNLVQVKVAMAGAQSIVPVQFDIEAKADNLVGPSPITASYMDIGNYDMKAAS
                     STVEKFAGRPTLWKVSNDANLDTSEAWTPVINANAGYMHGPDTGVPADHYLVSPTIDV
                     GTGNFTLSLKHRYSFETDTTVTPPDYYDGAVVEYSLDGKTWTDAKDVPGVIVMGGYTG
                     TLATYTGNLNPLTNRSAFAGATAGYATGAWVTSSFNFGTALAGKKLTLRFRIGSDNAS
                     GDEGWDIDEVAVTGANAPPFQSQVLQSAAAVCKAKPVFSITQADLKSIPTGFQVRLDP
                     TIANAPGAISWTWTQLDGTPVQLGNSDTPNVTFTAPDSGTVIRLKWAAVSAGVTLEGT
                     VAIEVVKAGDSSSGTAATGCQMGGQAAPSLWSGLVASALLLLGRRRRKS"
     gene            complement(59860..60246)
                     /locus_tag="PKL17_00545"
     CDS             complement(59860..60246)
                     /locus_tag="PKL17_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNK43238.1"
                     /translation="MKTLVRSVQQAALLVTLLTTGIFGGGCRKSEPPPPASGGPRRIE
                     VTVSNQGFTPPRIMGRPGEDLTLSFRYDKSAGECGREVVLPSQNIKKTLTEEKPTEIA
                     LHLPQEKGEVTFTCGMNMLRGAIVVE"
     gene            60426..60887
                     /locus_tag="PKL17_00550"
     CDS             60426..60887
                     /locus_tag="PKL17_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43239.1"
                     /translation="MTLQTLPDILTEASRRKSVIADCVLLIEEEVAKKGGLTGIAIKG
                     AFAIVKAVKPGFLAEAVDHMLDDFAARLDPFYQSQKQAAGRSLPSHFAENSGPIAEAL
                     LGITDQRAQRAQNQTLKKTYEKLRPTAKKHVEEAVPGIGRLVEKYAAKVAA"
     gene            60923..61615
                     /locus_tag="PKL17_00555"
     CDS             60923..61615
                     /locus_tag="PKL17_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK43240.1"
                     /translation="MAFTLGARFYAWQTWHEVWRQHCGSLAAHFPPPLPSGRLSVLDL
                     GIGPGVSGIGILQRRPDLWLVGLDFSADMLDLARHYLRISNHGFPLVHGDVTHLPFAD
                     ECFDVITHHSFLYLLGNRDAGLKEMARVLKPGGCYVMLEPNRRGSVLAVPKLGGPFRF
                     RLSMLLWRIVSTGFGQFLREELAALLETHGFVCERFEDTLGGMGFIVVAKKPLSSAPP
                     SSFPKPTDLQGA"
     gene            61617..62612
                     /gene="hemB"
                     /locus_tag="PKL17_00560"
     CDS             61617..62612
                     /gene="hemB"
                     /locus_tag="PKL17_00560"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNK43241.1"
                     /translation="MQFPEYRPRRMRRSDGLRRMIRETRLSVDNLVYPIFVCPGRDTR
                     RPISSMPGQAQVSVDQAVIEAKECFDLGIPSVILFGVPDDAKKDRVGSDAWNGDGLVQ
                     QACRAIKRACPELVVILDSCFDEYTTHGHCGVVMPEAGWPDVDNDATLENLGRVVVSQ
                     AKAGADMVAPSGMMDGFVTASREALDEAGYSHVGIMAYSAKYASCFYGPFRQAAGTAS
                     GFGHRKSYQMDPANSNEAIREAGLDEGEGADVLMVKPALAYLDVIAKLKAEYDLPIAA
                     YNVSGEYAMLKAAVEKGLLGEEQAVLEVLTSIKRAGADIILSYHAKDAARWLAKK"
     gene            62699..63718
                     /locus_tag="PKL17_00565"
     CDS             62699..63718
                     /locus_tag="PKL17_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK43242.1"
                     /translation="MKTRTYRWLRGLTAAALLWGGSGCLKLTLYNASVMKPSQVAVYF
                     SVTDSGGNPVANLTADKFRIYEDNGLVSVYESKQTILNPEVAAVHYTLLLVDLSGSIT
                     QSGAMEQLKPAVQSFADRVGSLQKVAIYGFDGSKEIFPIIGFTDGEGGVRAAAERLAS
                     FKGRDPSTNLNGAVIEGLKVLDKSLEHASTPLKFGSLVVFTDGTDRAKRVLRPDLDKS
                     LDEAQQKDTALYVIGVGKEIDSGELRNIGRTASFLSTDPAALAKAFDSIASRVEAYTK
                     SFYLLSYCSPARAQEHDLKVESGGGLSKGSLSYHFDAAGFGPNCDSNRMPKFDIHRPI
                     LKSKK"
     gene            63936..65405
                     /locus_tag="PKL17_00570"
     CDS             63936..65405
                     /locus_tag="PKL17_00570"
                     /inference="COORDINATES: protein motif:HMM:NF027993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43243.1"
                     /translation="MKMTLGTSLGLVAVAFAAACGEPEPSDSLDPVLKGEIEDALARF
                     DADPRSFMNALPQKVGAPSPAFSPSVVKSLDYISAKNARRQDGPADAPGSNDQAKNLV
                     DNLQLTNLADMHSIRSASLPESAWSSDYWAIYAGVIAKRYTDPNFPSSEDWKVLTDYV
                     RSKQPSNPDDLSPAEKYDLLVGDTSKTMTKHNLDEGQYYYSSYGKVETWMGICHGWAP
                     ASFMIQRPTKAIEVLAADGKTKIKFYPSDVKALASLLWANASPTTKFIGGRCNVKNPA
                     TDANGRIKDQACRDTNAGTWHLAVVNQIGKSQRSMVIDATYDYEVWNQPMYSYSYSYF
                     NPQTNKAYSTLSYAKVAVADYTTDKFKSYRAPGTTHIVGVSMDVRWTVETYPSHSTTD
                     SASRDAISGARYLYTLELDSTGKIIGGEWHQNAHPDFLWLAPPGTRAVATGEPTGTQP
                     VWDGKTAIPSTWQTAARTASSNGMPLANVVESLVRLSRL"
     gene            complement(65509..65874)
                     /locus_tag="PKL17_00575"
     CDS             complement(65509..65874)
                     /locus_tag="PKL17_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43244.1"
                     /translation="METGSAPTNPIERPDQTALDQKKHASLTSASKHSYGRRAPAIAW
                     EAVNLGWAPRIGLHFKVNLEQTPPFGPFFKVNLEQTPPLGLFFKVNLKQTPPFGPFFK
                     VNLGLKPRRRRKISQLAAV"
     gene            65991..66587
                     /locus_tag="PKL17_00580"
     CDS             65991..66587
                     /locus_tag="PKL17_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43245.1"
                     /translation="MKAQKAVIVITPSVAGGATQQQSNRRFATPRKSGGLIPVERDVL
                     SRVVSVWVDKQWFFGIEPIMMTPRPFFGVFQRKENSLAGRLATQIPAMLVALHTDEQK
                     VRPVISQPVIAGPFDACPAALLHSGLGAIVLDLKWPLLGFTIGAFVPLRGCERLPHVS
                     RCIDVLTKAEILVALALRAGGIVGRAEFLGQMPTFFPK"
     gene            complement(66614..68014)
                     /locus_tag="PKL17_00585"
     CDS             complement(66614..68014)
                     /locus_tag="PKL17_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43246.1"
                     /translation="MPTRTLGFKPVPAYLLSVTIENLRCFGPKQTLDLSDGSGRPRQW
                     TIILGDNGTGKTTLLQALATMQPYPSENVKKDGLWDLHWHWDRQSVANKSAKTVSISG
                     TYAIGSLLSQKSADFTSDTAGIEADEDSFRAGSLSSPNWRLALHAYGAARRLGTGPLS
                     KTPLWPDPVLDRVGYDAAIDTLFNPDAALLNAEEWLRDQHSIMLATKAGGTKTKAASQ
                     YREAVQVLRGLFPDDDIEDIRCVPIRPGSRTGFMEVQAKTPYGFVPLRQLSLGYQTTT
                     AWILDLAARMFVRYPDSKRPLEENAVVLVDEIDLHLHPRWQRKLFRQLETHFPNVQFV
                     VTAHSPLVVQAAGADANLVVLRRPEKARHVVIDNERTSVQNWRIDQILTSDLFTGVPS
                     AWAPEFDDDYGRQASLLQKRRRTAQEQADLDAIEKRLAARGAIGSPAEQRYLAILREA
                     AQSAQKPRRNQRDTRC"
BASE COUNT        12839 a        20298 c        21033 g        13964 t
ORIGIN      
        1 ttcattgttc gcgcccgtga tgcagcggga aacatcgaca aaaacacgat tgaagtcact
       61 gccacgacgc cggttcctga cacaacggca ccaacctttg gcggggtgac gggcgctacg
      121 gtggctggca acaccatcac gctcagctgg acggcggcca ccgacaacgt gtcgcaggcc
      181 gcgaacatcg tgtacaagat ttatcaagcg acgacgatgg gcggcgaaaa ctacacgacc
      241 gcaaactata cgtcgcctgc cggtgcgacc tcgtttgctg tgaaggactt gacgccaaac
      301 accacgtact atttcgtggt gcgcgcgcaa gatgaagccg gcaacgcatc gaccaacacc
      361 caggaaaaga gcggcatggc gctgacgccg acgttttcgg gtcaggtggg accgctgctc
      421 tccaccaact gcgcgggttg tcattcaggc ggatctccgt cgggaatgct cgatttgagc
      481 agcggcaaag cgtatgcggc gatggtcggc gtaaacagct cgggctgcgt aagcgtcaag
      541 cgcgtcgctg caagctctgc tgcaaacagc tatgtcgtgg gcaagctaaa cggcaccgca
      601 gtcgccgggt gttttgttgg atcgaagatg ccacccggtg gcggtctcag cacggcgcag
      661 atcgccacgg tgtcggcttg gataaacgcc ggcgcgctca acaactaggg ctgcgaacac
      721 gaggcaatat gaggaatccc agagactctt tttgttccta ctgcggcacc gagtttttgc
      781 gaccgctccg ctatccgcgc aagtgtcaaa acccaggctg cggggcagag acgtatgcaa
      841 atccgattcc ggtggcgctt ctgcttttgc cggttttgca caaggaacgg atgggtcttt
      901 tggtggtgcg gcgtgcgatt gaaccgcagc ttggcaagct ggcgctcgtc ggtggatttg
      961 tcgaagagta cgaaacgtgg cagcaaggtg cagcgcgcga agccaaagag gaagccggtg
     1021 tgacggtcga tccgaacacc cttgccccct actttttttc tagcaccgaa ccgcgtccca
     1081 atcgggtgct gctgttcgcg ctcggtcagc cgataagctc tgcttcgctg ccctcgtttg
     1141 tgccgaatca tgaagtgtcc gagcgcggcc tgattttcgg aacgcaggga cttgcaccgc
     1201 tgtttgcgtt tcacttgcac ctgctggcgt cggcgcgatt cttcgacgaa aaaggatcaa
     1261 acggtccgca ccagttcctg cgtctgtagc cgctttttcg ggtgcttcct ttcgtccgca
     1321 cgtttcctct ttgtcggctg tttccccgga gcttttctcg cgtaaagtaa agacatgtcg
     1381 gcgctgtctg tatcggtaca cggatccatt tccgagcttc agaaagaggc atggaatgca
     1441 ctgcttaccg attccgacaa tccgtttgtg cggtatgaat ttctggctgc gctcgaacag
     1501 agcggctgtg tctgtccgga aacaagctgg acgccgtgcc actttgccat ccgacgggga
     1561 aacacgctct gcgcagtcgt gccggcgtac atcaaagatg gcagtgatgg cgacttcagc
     1621 cgagattggg aatgggccgg cgcagcgcag cgggcgggca ttccgtacta tcccaagctg
     1681 gtgttaggaa ttccttttac gcccgtttgt ggtcggcgag ttttgcatcc ggaaggactt
     1741 cccccgcatg aaatcagtga tctgttttcc tgtgtgatcg cgaccgcaaa acagatcatg
     1801 aagcgctttt ctttgggcgc catccaggtg ctgtttcctc ccgcagatca gcaggagctg
     1861 ttcgtcgcac atgggctgat tccgcggatc gactttcagt atcatttttt caatccgggc
     1921 taccgcgatc gggacgaatt tgatgcgcgc ttttcttcca agcggcgcaa catcctcaag
     1981 cgcgaacgga gtgctccgga aagccaggga atccaaatcc gcacgctgcg ggcagaacag
     2041 cttttgcgcg atccggttca ctatgcaaag cttgcgcatg cgctgcaccg ctcgacggtg
     2101 gacaagctgg tgtgggggcg gcgctggctc aatgaagcgt tctatgtgca gctcttttcg
     2161 acgttgcttg caccgctgga actggtgatt gcacagtcgc aaggggacat catcgcgggc
     2221 gcgttcaacg tcgcttcgca gcgtcgcttg tttggacggt attggggctg ttttcgcgag
     2281 ctgccctttt tgcacttcaa cgtctgtctg taccacagca tcgacgagtg catcgcgcgc
     2341 aagaccctgg tatttgaagg cggcgctggc ggagaacaca agatcgcgcg cggttttgag
     2401 cccaccgaaa cacatagcgc gcacgcatat gccgatccat cttttcacgc cgcccttgcg
     2461 cgtcatgtga aaacagagag tgaaaaccgc cgtcaagcga ttgccgcgtg gcgaaaaaac
     2521 gatgcggtct taaaaccgtt ggttccgtcg attccctgac gtatcgtccc cccttgtgag
     2581 ccttcttcgc gcctacggtt tttcgctgct tttgcttctg tcgattgcaa tcggccttgt
     2641 gattggacag ggcgcgcctg catgggctct gcgactgcgg ccacttggtg atctgttttt
     2701 gaaccttgtc tttatgctga ttgtgccgct gacttttttc acggtgtccg caagtcttgg
     2761 cgcatcccca agtgggcggc gcttgaccaa aatctcgacg gtgatgctgc tcgtgttttt
     2821 ggccacgagt gtgattgcgg cggtttgggg acttgggttt atgtacgtgg ttcatcctac
     2881 gccgggggca gacatcgtgc tgggccgatc gagcgtgccc ttgcctccgg cgccggattt
     2941 tctgtcgcag cttgttgcga cgtgtaccac ttcggatttt ccgcagcttg tgtcccgcaa
     3001 gtcgatgttg ccgcttctgc tgtttgcagt cggcgttggt cttgcggctc gctccgtcgg
     3061 ggaacccgcg cttccgtttg tgcgtgtgct gcagggggga tccgccgtat tcatccgttt
     3121 gtttgatgca gtgatgtatg tcgcgccgat cggtctgttg tctttttttg cggcaaccgt
     3181 ggtggacact ggatcgcagc tcgcgagtgc atacctgcgg gtgttctggg tctatggcgt
     3241 ggtttcgctg ctctactttg gcgttggttt ttcgttttat gcgtatctgg cgggtggttg
     3301 ggaatcggtg cggcgtctgt ggtcgcagat gcttaggccg tcgctgactg cgctgggaac
     3361 ctgttcttcg atggcgacca tgccggtcaa tttggaagct tcgcgcgaca tgggaattcc
     3421 caaagatgtc tgcgatgtcg tgattccggt cggcgcggtg ttacacaaag acggatcggt
     3481 gattgggggc gtgctcaaag tgctgttcgc actgagtctg tttcatcttc cgcttacgcc
     3541 agggcggctc cttatggtga taggaatcgc aatcctcgtc ggtgtggtga tgggggcgat
     3601 tcccagcggc ggaatgatgg gagagcttct cattctctct gcatttggat tcccacccga
     3661 aacgctgccg ctcttggcgg tgatcagcat cctgattgat ccgctggcga cactcctaaa
     3721 cgcgaccgga gacaacgtcg ccgcgatgct taccgcccgc cttgtcccaa aggaacagcc
     3781 agactagctg tgcggatggc tctgcgcggc gagcagacag agccggaaca ggagccgggc
     3841 cccgacgatg gcatcccact ctgcattgcc cgacacttcg accagatcaa agccaatcac
     3901 ctgtcgtccg ctgtccacca ccatgtcgag caggaacacc gcttcatccc aggtaagacc
     3961 acccggcacc ggcgttccgg tttctggaca cagcgcgggg tccaggccgt cgatgtcaaa
     4021 gcttacgtac acctgctgcg gcagcgtatc gacgatgtgc tgacaggtgc gggcccacgg
     4081 ctcgccatgg aaattccgtt tgcgcagacc acgcatcggg aaaaagctca cgcgatcgcc
     4141 ggcttcacga atcgtctctt cttcttcgtc gcagtaatcg cggattccga cctgcgtgag
     4201 cttgctgaca tgtggcagat gcagcacgtt ttccatgatc gatgcgtgcg aatactggaa
     4261 gccttcgtat ccgcgtcgca gatccgcatg cgcatcaata tgaagtacgc caaatgactc
     4321 atatcgggtc gccagtgcgc gaatcagacc gagcggcaca gagtgatcac caccgagcac
     4381 gcccactgtc ttgccgagcg aaaggtgcgc ttgcgcttgt cgttccagcc agtcgtttag
     4441 ctcacttgac gccgcgttga cttcgccctg gtgcaaaagc agatccggat catcaccggc
     4501 cacgccttgt tccagtcgtg cgatgcactt ttgcgccagt tgtcgcagct tggcgttgcg
     4561 cttttgccac gctttgtctt ccggcagcat gtaaaaccca gcgcgccagc cgtcgggcca
     4621 caccggatca aacagatcca cctgggttga tgcttccagg atcgcctgcg ggcctttggc
     4681 ggttccggtt cgatacgaca ccgtgacttc ccacggcacc ggaatcacca ccacttggct
     4741 gttttcatag tcgaacggca gaccaaacag cttgccgttt ttgacgccca catcgctcgg
     4801 gctgaaggtc ttgtgtgaat cgctcttgct catgccggtt ttgattatcg gtcaggggga
     4861 tggtcgcgag ccaaagatcg atcgaccgac gcggacaatc gtcgcgcctt cctcgatcgc
     4921 cacttcatag tctgcggtgg tgcccatcga cagctcggaa agaccgagcg actcggcgag
     4981 cgtgcgcagc ttggcaaagt acgggcggtt gtcttccggc gtccacggca aaggtggttc
     5041 cggctgcggc ggcatcgtca taagcccggt caggcgcaga cccggcagct tgcgaatctt
     5101 ttcgcaaaac ggggaaagcg cagactgcgc gattccgctc ttgctgtctt cgccggcgac
     5161 gttcacttcg ataagtacat ctatgggcgg gccttggcga cgacagctca gctcctcggc
     5221 gatcgccagg ctgcacagcg cgtgaaacac cgacgctgac tttgcgacgt atttgacctt
     5281 gttgcgctga agcgttccta tcgcatgcca gcgcagcgtc ggcagatcag caagctcgct
     5341 ggctttgtcg cgcagctctt gggcatagtt ttcgccaaag tcgcgctggc ccgccgcata
     5401 cgccgctcgg atgagcgtcg acggttgcag cttggacacc gcgagcagcg tgacgtcttt
     5461 agggctgcgc ccggcgcgct gacaagcggc agcaatcttg gcgtgtacct ctgcgaggtg
     5521 ttgggcgatg tccgtcacgg tctggctcgt ctttgccggt caaaagcccg gcgtttccat
     5581 cggtccttgg ctgtggccgc tgacaaactg gcgcaccacg ggatcgtcgg tgttgcggaa
     5641 ctcatcgggc gtgccgagca ggtgaacgcg gccttggtag agcagggcga tccgatcggc
     5701 gatcgaaaat atgctggtca gatcgtgcga aacaaccacc gacgtcacgc ctcggctgcg
     5761 gctgagccga cggatcagcg catcgacgcg acgcgcgctc actggatcga gccccgtcgt
     5821 cggttcgtcg aacaaaagat agtcaggctt tagcgtcagc gcgcgcgcaa tggccacccg
     5881 cttgcgcatt ccgtcggaca ggagcgccgg aagctgatcg gcaaactcgc ccatgtgaac
     5941 ttcgtcgaga aactcaagcg cggctttgcg agctgcggtg tccgacatct gctggtgctt
     6001 gcgcagcggc agcgccacat tgtcgacgac cgacatcgag tcaaacagcg tcgagctttg
     6061 gaacaccatc gcgcagcgac ggcggattcg gtaaagctcg tgttcggaca gcttgtcgat
     6121 gcgttccccg tcgagccaga tttcgccgcg atcgacccgc aaaagaccga tgacgtgctt
     6181 tatcgcaacc gatttgccga cgccggatgc cccgatcaga aacaggatct cgcggtcttt
     6241 gacatgcagc gtcagaccgc gcaggacggc ctggcttccg aacgactttt cgaccgaacg
     6301 gaactccagc acggctactt gccgctctgc gcctgccggt agacgttgag atacgcacgc
     6361 gccgacgccg cccagccgaa atccgcccgc atcgcccgtt tttgcagccc caggaagctc
     6421 tgctcatcgc tgtggtaggc agaaagtgcg cggcgaacgg catggcccag tgctgcgacc
     6481 gaaaacggct ggaacgtaaa gccggtgccg ctgcgcgagc gcgcatcaaa gtcgacgacg
     6541 gtgtcataaa gaccgccggt cgcgcggaca atcggcaccg tcgcatagcg catgctgtaa
     6601 atctggttta agccgcacgg ctcgaatcgc gacggcatca cgaaaaagtc cgatccggct
     6661 tcgatgcggt gcgccaagcc ttcgtcaaac gccagccgca ctgccagtcg cgtcggaaac
     6721 cgctcggaca gcatgcgcag ccgctgttca aacgccgggt cgccttgccc aagcagcaca
     6781 aactggaact cggtgcgctg cgtcagctcg gactccagcc actcaatcag caggtcgatg
     6841 cctttttggt cggtcagccg cgagatcgtc gcaagcagcg gcgttcgtgg gcgcaggggc
     6901 aaagacagct cgcgctgaag ttcggctttg cagcgggctt ttccgacgag atcggcagcg
     6961 aacgcatctt ccgaaagggc gtccagctct gccgccggct tgccaaatcg ggccggcagc
     7021 gcgccgtcag accacgggct ccagcgatcg tagtcgacgc cgtttaggat gccgctcaag
     7081 cgggcactgc gcgctcgcat cacaccgtcg agaccgtagc caaactcggg cgtcgcaatc
     7141 tcctccgcgt agcgcgggct caccgttgtc aaccgatcgg caagctggag tccggctttg
     7201 atgaaagacg cttggccgta aaactcgatg ccgtcgggcg tgagcagatc gtcgcgcacg
     7261 cccaggtggc gcgcttcggc aagcgaaaac agcccttgga acatcaggtt gtggaccgtg
     7321 aataccgtcg ctggtggcgt atcccaatct gccttttgcg cgtaatacac cgcgaggccg
     7381 gcctgccagt cgtgggcgtg aatcacatcg ggacgaggcg tcagctcttt ggccagacag
     7441 atcgccgctt gcgacaaaag cgcaaagcgc agcgcattgt cgggatagcc ttcgccggga
     7501 ttgcctggcg ggccatagac accggggcga tcggcaaaca gcggatggtc gatgagatac
     7561 accatcactt gcggaccggg accgggcagg cgaccttcgt aaaggccgac ttcaaatgac
     7621 tggccaccga cggaaaccgc cagcttgcgg agccgcttgg ccagaccgtg ctgctgcgga
     7681 tcaatggatc cataacgcgc cgagacgacc gtcacccgac ccgcaccttg ttgggccagc
     7741 gcttttggca gggcccacag cacatcccca agtccgccag tcttcgaaaa gggcgcgact
     7801 tccgacgcta catgcagaac attcatcagc aaaaaccctt gtcgagcgtt acgcgccgcg
     7861 cagatgacca agtgccgaaa cgatgccact cggcatcgcg cgcaggccaa tcaccaacat
     7921 gtcgtcctcg gtggcaaact cgcgaatcgt caggatttcc gacaccgctg cgacgactcg
     7981 tttggccatc gcgtttgtct tcagcttcgg aagctggatc agatcgagca cgaactcatc
     8041 ccccgctgcc gacagaatga cctgcggtcg gaacggcaaa aacgccagca tgtcgcccgg
     8101 tttggacagg tccatcatca tcagcatcgc ggccatcggg ctgtcggctg gcggtttgac
     8161 ctgaagttca cggatgcgga ggttcacccg aatgctctcc gcggaaagct gcacaccgtc
     8221 gacggatacc accgccgaaa caaacagcgc cgttcccatc acattgaacg tcgccgaaaa
     8281 gcgcacaccc ggtggcaccg cgacgagcga cagatcctcg ggcagcttgc cccgcgagag
     8341 ctttccaagc agccacgtca ccgcgtccat cggcaaaccg aaacggaacc gcgtggcgtt
     8401 cacggcactg cgcaagagat agtcgggctt tgaaaccaag taggcaaaag cttgctggat
     8461 ctcttgtagg gtgatcactt tcatgctccg taagctagcc caaccgggct tgggttgtca
     8521 cgacggataa cgcaggattg gctggtcgtt tgtgcccgcg aaccgggcgg cgcaagtgcg
     8581 gtcggaatgg ggcagtgtgc ggctcagttg gtctggatcg gctcaatgac cgagatgcct
     8641 ttttcggtga gcgcccagac gcgaccgcgt acatccacgc ccatgttgag gatcttgtcg
     8701 tcgagcaggc ccgagcgagt gccaatctga tgggttcgct tttccgccac cacttcgatg
     8761 acgccgcgat cggtgccgat ccagattctt ccgtcggggt cgcgggccag ggcgctggct
     8821 ttgacgttgt acggcggctc tttggggaaa ccccagcggt tgccgtccca tacaccgatg
     8881 ccgcggcttg tcgcgatcca gatttgaccg tttagtcctt cgatcacgtc gtgaaccagc
     8941 tcggattcca ggccgtcgtt ttcggtaaag accttgacgg tcttgttgtc ctgcaatcgc
     9001 accgcaccgg cccccgaagc gagccatgct tccgtcgatc ccttccaggc aatcgcggtg
     9061 acatccgacg ggacattgac accttgcgag acaccaggct gggttccggt tggccgctga
     9121 cggtgataga ccacgcggct gtcgtcgacg tacacctcgg cggcaccgta agcgcgggcg
     9181 tcctgttctt tgtcgacgta acgcagaccg acccaaagct ggcctttggg cgaaaagctg
     9241 gcaaacgaca gatcgggaac gccggtcgga acctcgagcg gggtcagtcc gacgggcgac
     9301 cagtcgcctt tgttgccgac gcgcgaaatg cggacgctgc ggctcgatgc gccacggtgc
     9361 agcgcgatca ctgcgcccat cgggtcacga accaccgcca ggacgtgcga tcctttttcg
     9421 ggatcgacgt tggtgacttc gaacgtctgt ccgtcgaaac gccacgactg cgtgccgccg
     9481 gttgcgacgt agcagtcatt gttgttgatg caggccaccg aaagccgttc cgcgcccgcc
     9541 gtcaggtcaa acaggcgcag cggcgtctgc ttgccttttt ggatgcgcat cacacccaga
     9601 aagcgggtgc cgacgtacag cgagtcggcg tccgctgaaa ccgatgtgat gccgtcgggg
     9661 agccgaacat cgaccgcgtc agtatcaaac agcggtgcgg tggctgccgt gccgcccgga
     9721 ccggcaacca gccacggacg atctggcctg attgcagacg ctggctgggc cacggcggcg
     9781 tactggtctt gcgaaggaac aagcagcgcg ctcgtctttt tgccgccttt gtcctttttg
     9841 ccgcctttgc ccttggcggg ctctggtggc ggcggcggtg gggctgctgc tggcggtggt
     9901 ggcggcgggg ctgcttcttt gacgagcagg ccggccagcg gctgcgcttt gggggcgccc
     9961 atcgacggac tgcccgaaaa cgaaaacttg agcgggccgc gaacttctgc cggcgttgcg
    10021 acgcgcttca tcgtccatac cgactgaccc gcgccaagat acagttcacc gccgacgcgc
    10081 tggacccatt cgatgatgtt tgcgctgtcg acgcgatagg accaaaagcg ggtgccgtcg
    10141 taaaacgcaa tgcgctgacc accggggcca tcaccgacgg caagcagcga gccattgttg
    10201 tcggcgacca tcgcgcggac gttgtcgatg tcgttgccct cggccatgcc aaactggagc
    10261 atgtcgcctt tgacgacgcg catgatgcct ttgccgctcg tgccgatcca gacgccgctg
    10321 ccgccgtcat ggaccagcgc cgtgaccgtc gttcccgctg cgtagcgttc ccactggcct
    10381 tggtttagct tcgcaagtcc gtcgctgcca ccgacccaaa cattgtcggc atctggcgtg
    10441 acgacgatac ccgcgacgag gtcacccacg ggcgctttgg gatacttggt ccagcttccc
    10501 gatgcgaagt gtccgacgcc tttcgaggtc gtgacccaga caccgccttt gacatcgagc
    10561 gcaatgcctt cgatgcgatc cgccggcagc ccgtcgatcg tcgtaagaac tgctggcgtc
    10621 gggggttcag acgtcgtgct ccagcgtaca agtccgcgtg gcgtcccaac ccagaccaga
    10681 cctgcggcgg cggcgagaga cttcaccggg cgtgaatccg agaagctgcg gacgcggacg
    10741 ggggcactgc tgccgcccgt gtttgccgcc gtcaccgcgc caccaggctt tacatccgat
    10801 tttgcagacg gaccacagcc agcggtgaaa aactggctcg acaggaggcc caagcccccc
    10861 agcgtccatg cggtcacgga agacaggcgc ctatgcggac cagcggcaat ttgctcgtgg
    10921 gagggggttc gcttgttcgt gctcataccg atttcctgag gctgcgacgg ttccgctcgg
    10981 ttcgacgagg cggaaaaact gaaaaagcag cagcttgata gggcaacatg ccggatgctg
    11041 taggcgggtg tcaaggccga cggtaaggtc tgtgggtcac tcagaaaaac gccgcaaaac
    11101 gcgacactag ctgcgctggt ttagttccct cgcggcttga tcatctggaa gtagcgcttg
    11161 acgtagaagc gatagcccgg cgggacgcgc tgtttcgaca tcacttcttc ggacaccgcc
    11221 gtgtagtcgc cgtagacctt gcggtacgac gctgtcgcga agcctttttc tgctgcgccc
    11281 gagatcgtct gcgagcggct gggtccagcg ccttctttgc catagacccg cgtcagcttg
    11341 cgcttgcttt caatctgcgt cacatcaccg aggaagttcg gatcgtgctg tgtccctggc
    11401 cggtcaccct ctttgccttg ccccggtccg ccttggccgt tttgattgcc ctgaccgcct
    11461 tgcccttgtc cgggtccgcc cgtccctggt gacgcttgcc ctggcatcag cacgatcgtc
    11521 gtctgtccgt cttggccact tccaccgagc accaacgtct ggccatcgcc gtcgccgttt
    11581 gcccgttttt tgaaatcctg catgtctttt tggccctggc cttgaccctg gccttgaccg
    11641 cttttgccaa gccgccgcag cacctgcttg atctggtcga gcgtgacgat gttctgcttc
    11701 tgcccggctt tctgtttggc cagcttgcgg acctcgtctt ggtaacgaga aatctcgcgc
    11761 gacagctctt ccatcgcctg acgctgatcc ggcgtcattt tttccagcga ctgccgcatc
    11821 cgttctgccg cttgctgcag atcgcgctgg agctcccgca tctgcttttg tgcttcctgt
    11881 ttttcgcgca gctgtttttc aacctgcgag agctgctgct gcttttgcgc cagatccttt
    11941 tgcgcggctt tgtcctgcgg atttttcttt agctgttctt gcagacggcg aatgtcctct
    12001 tcgagctgct ttttttgctc tttgagcttg cggatttcgt cctcgaactt ttgctgctcg
    12061 cgctccattt ccgacagaag cttgcgtgct tcattttggt attcgggacg ctgattgcct
    12121 tccagtcgct gaagctgtcg ttcgcccgga ccgtcggcgt ttagcttttg ctcgagctgt
    12181 tcaagctggc gctgcgtttt tttcagccgt cgctcggtct cttcgtcctg cggattttcc
    12241 gccagcttct ttttgagccg tcgctcttct tcgcgcagct cctcacggtt tttttccagc
    12301 tcgtttcgct gctgctgctt ttgcgcaagc tcttgcttct gcttttcctg ttcttgccga
    12361 agctgctcgg cgagcttctg ttccgcttgt tttagctcgt cgagcttgcg ccgcgcttcg
    12421 ctgtcgtttg ggttcttttc gatctgctgc tcaagcttgc gtttttcgtc gcgcagctcg
    12481 tttagttttt ggctgatttc ctcggacttg tttttggcct gccactcgtc tttttggctc
    12541 gccccgtctt gcttgttcgt cggtgtgttt tcgtcgggcc ggctcgacat cgctttggcg
    12601 gctcgctcga acgcttcggc catctgctgt ttgtccgccg ctgccgctct cgccagcgct
    12661 ttgtccgcca gctcttttag ctccgctttc gctttgtcga gatcgtcttt gaccagcgct
    12721 tggccaagct cctgcgtcag tttcgattcg cgcagctcac tgccggcgcg gtgcagttgt
    12781 ttttccattt cagccagcgt cgcttcggtg ccttggcgga cgcgctgttc gatttcgctg
    12841 agccgttcga acgcttgctg ccgggtcagt tcaccccgct cgacttgctg gagcagatcg
    12901 agcatttcgc gcagaagctg tgccgtttcg tgatcgccgc gctgctcggc ctcggccagc
    12961 ttttgcaaaa gctcctcacg ctcgggcccc agaagttcag gatcgatgtg caacgtgtcc
    13021 gccatcgctg cactgggtgt cgatttggct ttttccgtcg gcagtttcgg acgctcaaaa
    13081 cgcacaaaca gcagcagtcc attgagcgcg acaagcacac ccagcagcgg cagaaatggc
    13141 ggttttttcc acggtgcagc gacggaaggc tggacctttt gggcgacctg cgcggcatca
    13201 tggaccgcca gttccgcgag ccctttggca aagtcggtcg gcgcaagcgg tgtatgtccc
    13261 gccgcaaact gaatggccga cgacagacga tcgtgcagcg cgtggctttt gtcgatgatc
    13321 cccgccaccc acagcggatc gattcgccgc agcgacagac ccactgccgt cagcagcaca
    13381 ccaactggac agagcagcgc aagtcgcacc agccaaaaac ggctgagcag ttccagccga
    13441 aacaagagca gcgcgatcag catgaccgtc gccgctgcaa tcagtccatg gaccacgccg
    13501 cgcgccgccg cttgcagccg gagccgccac cgcacctttg ccaccttggc catgacttgg
    13561 gccagatcgt ctgctgcaaa gcgaaatgcg ggcgactcgt cgcgaggcaa ctggtttccc
    13621 atgcgcaggt ttctttcggt aaaaagtgta aaggtcgccg gcccggttgg ctaccgccgc
    13681 agcctggatt gcgccgtttc aagcgctgcg ggcgtatcca catcgaccag tacttcggga
    13741 tcgtcgaccg gaacgcgcag cggcggatgt gtatcgcgca gcgctcgcaa agaagtcgac
    13801 gacggcagcg ccagcacatc ggcaaactgt tccgacggta gccaaacggg atgtccgccg
    13861 cgatcagcaa acgtcggcac cagccagcgt cgtcgatctg cggccagcag tgttttgatc
    13921 gtcgcagtct gcacgagcgg cacgtcgacg ggccacaaga gcacgccttc gacgggagcc
    13981 agcgctcgca gtccgcagct tatcgacgac aacatgcctt cttcgggggc cggattccag
    14041 accagatcaa ccggcgacgc ggatttctta atccagcgcg caatctcgtc gccgtgtggt
    14101 ttcgccaaca ccactttggg cgtgcggatt cccagctcgt cgagcaccga caaaattcga
    14161 tcgatcacca ccgagtcacc gagcggacag agcgctttgg gtgtgcccat tcgacgggat
    14221 gcacctgccg ataggatcag cgcgtcgatc atgccagctt ggatgcctcg gccagcagtc
    14281 gtggcgctgc ggtgcgtgcg gtttcgctgt gcggctgtgc ggcgggtttc gctgcgtggt
    14341 ttcgacgggc acttcgcacc gcggtaagct cggcggcaat cgccagcgca atttcagccg
    14401 gggtttcggc gccgatcggc agaccaatcg gtgcatggat ttgggcaatc tccgcttcgg
    14461 gaaaaccctt gtgaagaagt cgcttttgaa acgtcgtcac tttgcgctgc gaaccaatca
    14521 tcccaagata cgcgagcggt ttgcccagaa gctgttcgag gatggcctga tcggtcgcat
    14581 gctcgcgcgt caccaccacc acatacgtcc gcttgtcgag cgggatgtct ttccagtcgc
    14641 gtacatcgaa gctgtcgatg atctgggtat caggaaagcg ctctgggttg ccaagctcgg
    14701 gcaaatcttc gacgacaatc gggacaaaat cgagcggcct caaaagcgga accagcgctt
    14761 tggcgacgtg acctccaccg cagacgatca ccagttcatc gggaaacatg ggctccagca
    14821 ggatctccat ttcgccacca cagcacatcg ccagctcgtg tgtcaggtga taacggacgc
    14881 gctcgcactg ggctttttgg ccgagcgcaa gaagctccgc cgcgcgaagc agcacctgcc
    14941 gttcgacgcg cccaccgccg atcgtgccga cttggtcgat gtgacctgcg actttgcgaa
    15001 tcagcatgcg cgtggcgtgt ttgcgcggtg tacttccagc cgtcctgacg acggtgcaaa
    15061 gcaccatgct ttcacgggct ctgctcgacg caagcagtgc ctcaaagacc tcaacctcgc
    15121 ggctcaccaa acgctccgaa acgacaccgc ccaggtctta gcacagcttg gcgttcttgg
    15181 tcagggcgcc cagcttagct gctgcgtggt ccgagcgctt ctttgcgcat catcgcatcg
    15241 acgaggaaaa acagcgcaaa gtaggcatct tcgatggtcg agcggctgga ttcggcgctg
    15301 tggccggcct gtcgaaagcg cagataacga tccgcaggca gtcccagcac cagtgcacgc
    15361 caggccggtc cttcatccgg ttcgcgcgcg gtatcgctca cttgcagcga aaaaaagatg
    15421 ccaacggtcc gtcggacagc ctgcacgtac tgcgtcgcgt ggatcagccg ctcgacttct
    15481 tgcacttgcg cgcgctgcgg accgggcgca agcagcatgc tgaggccttg atcgcggccc
    15541 gcggcttcca tgcgcggcgg ctgcggctgt agcggcggtt tgctcggctg cgccagccgc
    15601 gatcccggca tcgtcttgtc gagcgccgcg gcaccatgcg ggcggggcgg ggcggcttgc
    15661 ggcggcgtat ccacggcaat gggacgcggc ggacggcttg cgcgcgggcg gatgatttct
    15721 tcgaccgcgg ggagctgttg accgtcggcg cgaacggctt gcaccacttc gctggactga
    15781 tcgggattgg gcagcggcgg tgccgacaga ccactcatcg gcagaatctc ttgcgagcgt
    15841 ccggcctgtt cccgcagcag tgccgattcg tcgatgcgca gtggctgatc gagcgtgtag
    15901 cgctggacca ggctgcgcag tgcggcttgg gcttcgtggg ccgccagcat tcgacgcgcc
    15961 gtcaccatgt cattgccaaa cgacagatcg ctcgtcggca tcgaaccggt ttgacccagc
    16021 tcgggccgtt tgcgcagcgc actggctagc gcttcggtgg cgagcatccg gaacgaatca
    16081 aacacccctt ggcccgtcgc tgcaatcatt ggaactgact gcgactgccg gatgccgaac
    16141 gccggttcca gatccgacag cgccaccgcg tccgcgagat cgcgcttgtt ccatgcgacg
    16201 acgagcggca catcttgaag ccgcatcccc agatcgagca ggcagttgcg cagctcctcg
    16261 agcgcggcgg cacattcgtt ttcgcggcct ctgcggctgt cccagacaaa cagaattccg
    16321 tcacaacccg cgagcagcgc ccgtctggtc gattcgtttt gtacgctgcc cgaaccggca
    16381 aagacctgcg cgtgtaccgg ggctcccaaa agcggtggtg gcggcggata aaagtcgaaa
    16441 aacagggtcc ggtcggcacc tgtgttcacc gaaacaattt ggccgcgtac ctcggggcgc
    16501 agcagcttgt gaagctgtgt cagcgccgtg gtttttccag ctcctgctgg cccgtaatac
    16561 acgagcttga ggagcacttc gcgagcctgc gaataaagct gcgccacaat ttagcagctt
    16621 atcacatggg agtttgtgcg gttacgacgt ttgcagcgac gggattagcg gggcaaaccg
    16681 tggttcgaaa ggctgcaaac caggcgaagg acgaggacta gggcaggtag atgcgggtga
    16741 gcgcctgacc gcccgcgtag tggcagaggt tgcaatcgac gcggactgtg gcgccggtgg
    16801 ccgggtcctg accgactttc tggatcatgg tctggactcg ttttgcggtg ctacagtcgg
    16861 ggccggtcgt ctcacagacg cgggcaatca tcggcgcggt aaacttcgtc gcggtataga
    16921 agttaccgac ggagttggtc gttagacgtc ctttcggctg gacctgaccg tacttgtagg
    16981 cgccttgcgg atcgtcttgt ccgtagagga tctcgaccac catgtttggc aagccctcgc
    17041 cggaattaca gcccgaggtc ttgtttgcaa acagcgttcc cgagacagtc cacggtgagt
    17101 tttttgcctg tccacctgtg ctatggcatg cgccgcacac ccgaccgggc agcatcgtgg
    17161 cagttgccat tttcatggca tcggtagggt cttccccctg acaactggcc tgcgcttcgg
    17221 ctggaactgt cagtccgtcg gtatctccgc atgcacttgt cccaagcaga gccaggccca
    17281 ggcccagagc cgacagccaa ccaagcggcc ttctcccacg atgcgaactc atattgacta
    17341 ctccttttct gtcatcattg gatgtaaatg cggtccgccg ccaacccact ggcgaaatgg
    17401 caactcgcgc agccggtggt gaggtcaact gctccggtca tcttgagcaa catcgcgccc
    17461 ttcgagactc gagcggttgc gtttttgaag gcggcgttcg tcgggaagtg gaaatttccg
    17521 acggaattcg tcatggtgct ggcgatgacg ttgcccgcgg tgtcaaggat gtcgactttg
    17581 acgtcggcaa ctccacccgt gttgcaggtc ttgctgttct tggctccgta caccgtacca
    17641 cccgtgccga ggatcttgtc ggcggcttgc tgaccggctg cgtggcaagt catgcagttg
    17701 cggcccggta gcattgcgtc cgacgccttc agcatctcgg cggttggctc ttcgcagccc
    17761 gctttggctt gcggtgccgc attgaggtcc gcggtatcgc cacaaccaac accaaacagg
    17821 ggcatcgcaa acagcagggc acaagcaatg gttcgtaggt tcatcgtcac atctcctttt
    17881 ctcagcagtg cgagcgcgtg catcctacgc tttgtgcccc cacgccaatc ttatcttcaa
    17941 agcctaggtg gcgaataggg ggccttgcgt ttgtgcggcg gcgctccttc ggtgcgacat
    18001 cgcaaaacgg agtggttctg cgattcgacc agtggtgcgc ttggtttctg cctctgcgag
    18061 agaaggcctg ttttcaggcg ccgcttgtcc cgcactttgg ggtttggcac ctgccttgct
    18121 gattgcagcg gcatgaccaa cgaccaaaca tcgacccctt cctcttcttc ttcaccgggc
    18181 accgcgtccc accgctcgat tggcattgat gccatcgcgt ttgcagtgcc gcgggcctac
    18241 gttgagctgg ccgatctggc ggctgcccga ggcgttcctc cgaccaagta cattcaaggc
    18301 ttgggcgtcg ttcagatggc tgtggcccat ccggacgaag atcccgtcac gctcgccacc
    18361 gaagcggcgc ggcgctgtct gatggcggcg gggcgctctc cagcggaaat cggcctttgc
    18421 atcgtcggta ccgaaaccgc ggtcgatcac tccaagccgg tgtcgtccta tctgcacgga
    18481 ctccttggtt tgccgtcggc gtgccgtgtg ttcgaaacca agcatgcttg ctttggtggc
    18541 actgccggtc tgctcaatgc ggtcgactgg ctggcggcgg gctctgcacg gggccgctgt
    18601 gcgctgatcg tctgcaccga cattgcgcgg tattcggttg gcactgcggg cgagccgacc
    18661 caaggcgccg gcgcggtcgc gatgctgatc agcgaaaatc cgcgacttct tgagctggaa
    18721 ccggggatca ccggctgctt ttcacaaaac gtccacgact tttggcgacc gctctatcga
    18781 aaggatgcgc tggtcgacgg ccagttttcg gtgcagtgct acctcgacgc gctcgctgga
    18841 gcctatgccg aatggcagcg cctgagtgaa agcagcgagc cgctcgtgcg cacctgctac
    18901 cacgttcctt acggcaaaat ggcgcgcaag gcccatcagc gccgctgcca gctcgacggc
    18961 atgaccgatg aagccgccga caagagcttt gccgttgaag tcgagccctc gctgcgggtt
    19021 ccttcacagg tcggcaacat ctacactggc tcgctttatc ttgcgctcct ttcgctcctc
    19081 taccaccaag cggcggagct ttcgggcaag cggatcggac tatttagcta cggctcgggc
    19141 tgcgccgcgg agtttttcgc tggccgggtg atgccgaccg ctgcggccca gatcgcgcgg
    19201 gctggtgtcg atacaacgct cgccaatcgg catcggctgg acattccggt ctacgaggag
    19261 ctgcgcaaaa acgacattca tgtcgatcag cgtccatccg aaatgccgtc agccgcggaa
    19321 accggtgccg cgctcgccat ggtcgatcga tcgccgcgat ttaacggcat cgacaaccaa
    19381 gagcgccgca tctacaccgt cgcctaattc ggcaagtgac ggcagcccgc tttgcctgct
    19441 ccgcatcgga aaagcgggcc cgttcaagaa aaaatcagcg cagagaggga tttttcttta
    19501 cagcgaaaaa tcggccatgg tagtaagcca atcctctctc gcgttttggg tgcatagctc
    19561 aggtggttag agcgcagccc tgataaggct gaggtccctg gttcgaatcc aggtgcaccc
    19621 actcgttagt atcccttggt atctaaggga aaacttggtc ctttgggtgg ggcaggtttg
    19681 gctcggtggc gatgccggcg tgtcttcgtt gccttgctcg tttcctcagt tcccgtttcg
    19741 ggctcggctg agggggcttt tttcgcgact gcgtctgctg gttccgctga tgcgacgctt
    19801 gcggtcacgg gcgctggttc gctgcctggt cgcgcttccg gtacaaacag tactttttgc
    19861 gcggcttgtt ctctttgggc ggtttgttga ctggcgaagc ttggtgtggg cggcggtcgc
    19921 tttcggagcc agacgcccca cagcacggtc agaatcgcaa ccacaagcga tgccacagcg
    19981 agcgttcgcg cgcggcgtgc cgcagtctgg tgtgcaccgt tggttagcac cgccgttgcc
    20041 attcgcaaag gccgcgtcgc tcctgaactg ctgatgaccg tcggaggggc tcctgaatcg
    20101 ccaggcgcga gcgtaggcgg acctgaccca gtagaaacct cgccgggact ggctgctggc
    20161 tggggcaagg cgggccgaat ggtctgacca cttgtcgacg gaaggaactg gatcaggatc
    20221 tggagcgcta ccgcacaatc gacgggccgg ttttccaggg actgcgacag aaactgatcg
    20281 agcgcgggct gcaagtctgg tggaaacgta gtgcctggca gaagctcggt cagacgcagc
    20341 ggcggcgaaa ccatgcgcgc catcaccatc gacacttccg atcgactcgt ttccccccac
    20401 ggcagttttt tggtcagcat ttccgtcaag atgaccccga cggcaaacac atctgattgg
    20461 gggctggctt cgtcgccttg cagcgtttcc ggcgccatgt agtgcggcgt tccgagcgct
    20521 gtttgcgtcg atgtcacccg tgtcttgcga ccgagtacgc gcgcgatgcc gaagtcgagc
    20581 accttgacga cctcttccgc cccaaccgaa agcggcttgc gcgcgacgaa gatgttgtcc
    20641 ggtttgacgt cgcggtggat gatctgccga gtgtgcgccg ccgcgagtgc acgcaccgcc
    20701 gggatgagca gctccagcgc ccgcagcagc ggcatcgggg cttcccgctg aatcagtcgg
    20761 tcgagcgttt cccccccgag cagctccatt gcgatgtagc tttggccgtc gctggtgtgg
    20821 ccgctgtcga agatggtcac cgcgtttggg tggtccaggc tgctcgcaat ttgggcttcc
    20881 cgctcgaaac gtttgcgcat ttcggcgctc tgtcgatgct cgctgcggag cagtttcagg
    20941 gcgacgcgac gaccgatgcc ggcttgggtg gcgacgtaga cctcgctcat gtggccgtag
    21001 ccaagcggct cacccaagac gtacttgccg ccgaacacgc gctgatcgcc aagtagttgt
    21061 gcgccatctt tggcacaaaa attggcgtcc ccgtcgggtg gtagataaac agagtggcaa
    21121 ctcgggcagt atttgcccag cacggtgggt ggcggatcga agttttgcgc cacgttgtgt
    21181 gctgcagttt ccgtcatgcg tccgctcagc gagggcaggg ctgcgcgccg tcggcaagcc
    21241 cccttttttc tacgatagca ggatcgaccg ccggctgtct cgtcgcccga acgcggctgt
    21301 cagttccagc aagttggatg tttgggccag gccgaacagt gcggatgcgc gcggcttttt
    21361 gtgtcactgc ggacttcgcc gatacaacga ccgtttttct gcgctcgcca agcgaaattc
    21421 ctgcggctca agcgacggca aatcgcgata aacaattggt tgagttcgca tttttcgctt
    21481 gccgcgaatc tattgaatgg aatacattca agctatcaat tggggcggca cggatgggcc
    21541 aaaaacgcga cgagaagctt tcgaaagcga ggaatggcag ccgtgagacg acaacgagga
    21601 acgaagctgt gttgagcgaa gaacttctga caccaagcgc gcgaacgcca tcccgactgg
    21661 ggatgagccg tcgtccgctg cgtggtcggc cagttcccag tcgctcacgc agtcatttgc
    21721 tcgaggccga gatggtcgaa atcgagcagc agcacccaga cgggatgacg cttacgcagg
    21781 tgctttcagc gttcggtgat cgcgggattc atctgtcgga agcgacgttt cgcaaatacg
    21841 tgcagcttgg tctgctgggc cggagccggc gcgtgggacg caaaggcaag caccagggtt
    21901 cgctcgggat gtatccggcg acgacggtgc ggcggcttaa cacgattcgg cagatgctcg
    21961 gggcccacta caccatcgaa gacatccagc gctcgttttt gcgctttgcc gacgagatcg
    22021 acagcttgca gcggggactt gcggggctgc tcgatggtct ttcgcgggag ctcgatgggg
    22081 gccagtttgc agccgatcgg aagcgcgcga tgacgaaaga gctgctggcg attcgcaagc
    22141 tcggcagtga tgtggtgcag cgcattgagg cgctcgagcg agagctggtg tcaccgctcg
    22201 aacgtgcggc acgacagcga acctttggca caagcagcgg cagcgacgaa cttttgtaga
    22261 tgaggtggtt tttccaccgg gcggaatgca gggagacgca gtatgcaaga cattacccaa
    22321 ccaccggacg cgactccgtc ggagtgcaat gaaacagacg gcaacacgac ccccgaggtc
    22381 agccgcaagg ttcgcaagca tcacaagcga tcgcgcagca agacgatagc gccgcggcgg
    22441 ctcaccaaag aggagcttcg cgaggctgca cttctcccgg tgatcgacga ttcagaacgt
    22501 ccgcagacgc gggccgactg cgcgggtggg gcgcgtccgt gcccgtgggt gtcttgcaag
    22561 catcacctgt ttttggatgt gaatccggaa accggcagca tcaagctcaa ctttccggac
    22621 aaagagctga ccgagatgcg agagacatgc gcgcttgacg ttgccgatcg cggtggaatt
    22681 acgctcgaag aagtgggcgc gattttgaat ctcacccgcg agcgcatccg ccaagtcgaa
    22741 gtccgtggtc tgctcaagct gcgcgccgtg agccccgatg gtgacgtcgg gcgtgacatg
    22801 ccctagccgt ccccctgcca tccccgacct tttttcactc tgcggtccgc tcgggccgtg
    22861 acgacttcca ccccaacatg aatcgcgctg cgccgctttg ctgctgcggc ctgctttttt
    22921 cgcatgcgaa agcgacgcgc tctctcgatg agagcgggcg taggttgtgc ggctttggga
    22981 gcgagagcgc tgcgcggagg gaggcgggga ggttagtact ggtagccgat ctgaacgacg
    23041 ccgccgaagt aggtgtcgct ggcgccggca gcgaggtggc gggcatcgcg gcccgtcgct
    23101 acgtcacccg gcgtctgatc cacgagcgtc ttgcccaggt cgaacgtgta gttgaatagc
    23161 tcgtagtagc cgctcagccg gatcgtcaga cccttccaca ccatcgcttc gaggagagtc
    23221 aggctggcgc gcagaccgta gccgctcgcc gggccaaagc caccgttgcc gtacaaatcg
    23281 gcaaccgggc ccgatgcggt gtcggtgttg gcggtgccaa agcgctggcc aatttcaccg
    23341 tagcttatcg gaatgtggac gttcacgtcg acgacaccgg cgaagtagac cttgtcgttc
    23401 acaaaatacg ggaagcggcc accaagaccg agggtgaagt actgatacag gatgtcgggc
    23461 agaccgtgat cgtttacacc gtcggcggtc ttgtcgatga agttttcatc ctggtacggg
    23521 tactgcttct gctctttcgc cactgcaaag aagtgcgcgc cgtactgggc attgaacaga
    23581 atcgacggca tgttccgctt gttgagcagg ttgatgtgat agcggagacc gccttccacc
    23641 ttccactcgc gggtcgccaa aagaacctgg cttgtgcccg agccgtactt cgaatccggc
    23701 cagaagaagt agtcgaacgt ggcaccaatg ccgagacctt tgatggcgcc aatccgcatc
    23761 atcgccagcg ggtaggccgt cagatcgacg cgcacgccgg ttgcaatcga cgtcgcaaag
    23821 ccagggcacg ccgcggtggt gccgcgctta tacggcagat agtgcgaaac gtagccgcga
    23881 ccgagcggca tcttgggatc gggaacgcgc ttttccaggt cgtagcactt gagcggcgag
    23941 tctggacttg agtcttccgt atagctgaag ctacggccac cgacgatcac gcccgcgccc
    24001 gcatcaatga acggatagta aatcgggcga agcagctcgc gctctttgcg gcgtttttcg
    24061 gcctcgtcgc gagcgagctt ggcaatcggg tcttcttcct tgccgagctt ggacgttggg
    24121 tccgatggcg gaggttctgc ctgggcaacc ggtgctggcc tgtcttccgg tgggccggca
    24181 attgccgcga ctacggcggg accaatctcg ctttcgaccg tggtgatggt ggccggctca
    24241 accttgtcgt ttttcagatc gtaagtgagc ttgccgaccg gcttgccgct cgcgccatgg
    24301 cgggccgcga tgttgagctt gtattgaccg gagtctttgt cggtcttgag cttgccggtg
    24361 atgacgacgt cgatcttctg gtcattggcg atgagcgcga cgtcttcggt accgtggccg
    24421 gtgacgttga gcttcttggc cgccgcgttc cacttggctt ccggaacgac ggtgtagtcc
    24481 ttcttgagcg cgttttcgat cgccttttgc agcttcggcg gcgctccctc aatcgggagt
    24541 acgacgacgc tctttttttc ctgtgcggcg acgacgctgg gatgaagtga cagcgcccca
    24601 aatcctgtca gaccgaccag cagggcgcgg cccactgtcc gagctgtgag gaaggacgcg
    24661 agaccgaatt cgattcgatt gggacttctg cgcagcaagc tcaccatggg gtctccacga
    24721 aagacagagg gcagctcatc cggaacaagc ggtttctgcc acaggccaat accaaacgtg
    24781 gaagcatagt atcacaggaa tcacctgcta cttcggggaa tctctgcaca tcccttgtga
    24841 tagattttcc cgacatggtg ccgttggtgt gtcccacctg ccatacggag tctccggaag
    24901 ggagccgctt ttgcagcatg tgcgggcagc cgctttcggg ttcgagcctt gtgcgggctt
    24961 catcccctga tagacgggtg cagacgtctc ctgtgggaac ggtgctaccg agcgcggtgc
    25021 tttcggcgtt gtcggcggca tcatcggatg gccagcggac ggtgtcagcc cgcgcagcaa
    25081 gtgggtccag cggggcgatc ggggatcttg cgcaatcggg ggaaacggtc aaaacgatcc
    25141 gcgacagcag cgattcgggg gcgctgggtc gctcggtccc gcgtgtcgtg gccgatgagc
    25201 caacgctgcc ggtgcgctat cgtccgacac gcaagcgcag tgcggcacgg gccctgcgat
    25261 cggttttgtt tggtcttttg tggctgctct tggcggggct tgtcgcgatg tctgtggtgt
    25321 ggcttttgcc cgaacgccag gcggggccag cgaaagagac gccggcgacg gtgattgcgt
    25381 ctgtgaaaga accgagcaag ccaccgcgaa tgactgcccg accggcggtg ccggctgtac
    25441 cgaaagcagc gactccaagt ccggttgccg ctcctggctc cgaatcgccg cgagtaatcc
    25501 cggctcccga ttccgtccct aaagcccctg cggtgccatc gcctgtacca gcggaagcgg
    25561 tcgaaaccgt ccattcccca tcgactacgc caacgtcgac cgacttttct gccgtactca
    25621 gcggcgcggc gcgggcggcg tggcagcagg atctggccaa cattgagttt gtggcaacca
    25681 cctatgccgc acaggtccgc gcttgctacg agcggtcgat ccgcggcctt ggtggagagg
    25741 ctcccagcgg gcgcgttgtt gtgctgtttt cgctgaccga tgacggaaaa gcgcaacagc
    25801 ctgtgatcag cgaagactcg ctcggtttgc cggtgctggg accgtgtctg acccagcgac
    25861 tttcagaatg gcgctttcct cgccccgtag gtcccattcg caccttccgg tttccgtttg
    25921 tgttcgcggg cagtcgtccc taaatccgct actttccgct ttcggttcct gattttgcga
    25981 ttccaagccg cccaaaattg ggctgataat ggagcaacac agcgctgaaa gcggtcgctg
    26041 tgcttggggg tctttgacca tgagcggcaa gacgggggac gaaaagcagt cttcgacact
    26101 ggtcgcgtcg gcgctggtga tctcgcagcg agagcaggtg caggccgagc tacagcggct
    26161 gcttgcggaa gctgacgtaa acgccgtctt ggtcagcgac tcggattccg ctcgggcaca
    26221 ggtggccaag acgcagtatc cggttgtgct gattgatgtg gactcgattt ctttgtcgca
    26281 agccgtggtg ctgcttggcg acgtgcgacg tttggcgccg cgggcggagc tgattgtgct
    26341 gctcgatgag ccgaacttcg acgcagcggt acaactggtg cggcgcggtg ctgtcgacgt
    26401 agtctgtcgt tcatcggacg atttgcggct gctggggccg cgggtggcgt cggccatgcg
    26461 cgatgccaaa cagcgagcgg atcgcagtca gatgctgcgt gactacagca tcttgaccga
    26521 agacctcctg tatcggctga ccgatactgc gcgacggatt ggcgagctca aagcacagat
    26581 ccatagcagt gccaaaggat cgggttcgcc cgcgtcgccg attgacgatt atgtcagcgt
    26641 tctgatcgtg gaagaagacg gctggctgtc gcagtcgctt tcgccgcttg tgtcgagctc
    26701 gttttcgctg acgacggtgg taagcggtgg tgcggcgctc gatcgagtgt cagaacggcc
    26761 tttcgacttg gcacttatca aggaaacgct gccggatttg ccgagtcgga cggtgattcg
    26821 ttcgctgcaa gcgcagtcgc ccgaaacgct ggtgatgctg tatctgcctc cgacgcgcgg
    26881 caagccagga cggatcgatc ggctggaagc cggtggcggc aaagtgacga cgctggtgag
    26941 cgcgtttaca tcgcccaaac agctggccga acggctctcc gagcttcagc aagcgcaggc
    27001 ggcacgccga cgcgagcgcc gttatttggc cgagttccgc caagaaaact acgagctttt
    27061 gcggcgcttg tccgagctgc gcaaacgcta ccgcgaggtc gacagcacac agggacgagg
    27121 caagtaggcc cgcatcttga ctgacctagg aaaatagcgt gtcataatac tgggtttttc
    27181 tatcgctaat gcgctctgac ctctctttga aatcagggtc caaaggacgg tcggcttggt
    27241 cccaagccgc gcgacgtgcc gtatgaacga tgttccaacc acgggcgtca ctcgggtgtg
    27301 cgtgacctgc cagcaggtct ataactacaa agaccagcac gtctgtccca aaggcggcaa
    27361 agccgaggtt ccgccagacc ctctggtggg ggccacgatc ggcgggcgct accttatcga
    27421 gtcgtttttg tcctcgggag ggatgggcgt agtctatcga gccaagcata cggtgctcga
    27481 taagccgctc gcgatcaagc tgctgcggga agcgcaagat ccgatgatgc agcagcgctt
    27541 tctcctcgaa gcgaagagcg cgtgccacat cggacacgaa aacatcgtcg acatcaccga
    27601 tttcggtgtt cttcacgatg gccgtccgta tctggtgatg gaatttttgc agggccagtc
    27661 gcttgaggcg gtgattgcca aaggcgcgct gccacctttg cggacctgcc gaatcgccga
    27721 acaaattgcc cgcggactgc acgccgtcca cagcaagggc atcgtccacc gagacctcaa
    27781 accggggaac atctttatcc tcgagcgtgg gcgcaaagac ttcgtcaaaa tcctcgattt
    27841 tggcatcgcc aaggtgatgg ccggtacgcg cgagtcgttg tctggtggca gcgcgaattt
    27901 gccgaatttg cgggcgacag cgacggggac ggtgttggga acgccggaat atctgtcgcc
    27961 cgagcagggt gccggggaag cgttcgatgc ccgcgtcgat caatacgcgc tcggctgcat
    28021 catgtacgaa atgctgacgg ggcaggtgcc tttccgcggc aatggaccga tgtccacact
    28081 gatgaagcat ctgacggaaa agccgattcc accgcggaag cgccgtcccg agctgaacat
    28141 ttccgaaacg ctcgaaaaaa tcgtgctcaa ggcgatggcg cagcagaagg aaagccgcta
    28201 cgccaacatg gacgagctgg ccaacgtcct tcaggaactc atcgacagtg ggacgctgcc
    28261 tgctgatccg gtggttcctc gtccgtcggc gtcgctgcaa acggtggcgg cgagtctgtc
    28321 ggcttcgtcg gcgccgccgg ttgagctgtc gccgctgaca tcgggcgata tgtttgcacc
    28381 gacgctgcaa gccgcgattc cgaccaaaga cgagatcgcc aaagagcgcg cgaagacaca
    28441 gcccgaatcg accaccgcga cgatcgatcg cggcaacaaa ccaccgtcag agaaccgaga
    28501 aaaagcgccg cttgcgaccg agcaggttcg tcccatcgtc cttgcgaaac cgaatcggct
    28561 gccgctgctg ttgctcgggg tggcggcggt gatggtgctg ctgctgattg gcgtgcttgt
    28621 cgtaaggctt gggcccggca gcaaaccgtc acagcctgtg ccggcggtgg accttggcga
    28681 acgcaaagtc gaaacgccag tgccggttga atcgccgccc accgatgagc acaaagtcga
    28741 gctggaaaac aagacggcgg gcaaagtgac tgttctgtgt ggtccgaaaa aaagctgcat
    28801 tctcgacgcg ggaaagacct gcgagcttgc gctttcgccg gacgcgcgat gtgaagcgag
    28861 cgccactggg tttgtcagcc gcagcctgga tgtcggaagc gccgtcaagc acgcgggcaa
    28921 aggacaaacc gcgcatctgg cggttcacct tgacgccgaa aagccggtgg gcaaagtcaa
    28981 aaagtccaag ggctccaagg cgggcaaggg caaaaagcgc taagccaagc gcgctggcga
    29041 tgacgcggca tggtcggcgg ttgcctccgg ggggcgatct gcggtacacc gcttcgcatg
    29101 gagactgctg ccaagaccga acctacgcac ccggtgcctg cgtctttcga gacgtcgcct
    29161 gaccgatacc gccactggcg gctcgagttc gacggggatg ttgcccgttt gctcatgtcc
    29221 gttcgcgaag acgagccgct tgttccgggg tacgtcctca agctcaactc gtatgacctg
    29281 ggcgtcgata tcgagctgtg tgacgcggtg cagcggctgc ggtttggcca tccggaagtt
    29341 cgcaccgtcg tggtgagctc gggccgcgat cgcatcttct gcgccggtgc caacatccac
    29401 atgctggcgc agtcgtccca cggctttaag gtcaatttct gcaagttcac aaacgagacg
    29461 cgcctgtcga tcgaggacgc ctgtgcaagc agcggccaaa agtatctggc cgcagtctca
    29521 ggcccctgcg ctggtggcgg ttacgaactg gcgatggcgt gcgacgaaat cgttctcatc
    29581 gacgatggat cgagcacggt ggcgcttcct gaagtgcctt tgcttggcgt gttgccgggg
    29641 acaggtggtc ttacgcggct cgtcgacaag cgcaaagtcc ggcgagatcg cgccgacatt
    29701 ttttcgacgc tggccgaggg cattcgtggc aagcgcgccg tcgagtggcg tctcgtcgat
    29761 cagacggtgc cgcgcagccg ttttgcccaa gccgtgagcg atcgggccca caaactggca
    29821 caagagtcga aaaaccaagt cgtcagcgca cccggaccgc agacggggat ctcgcttgag
    29881 ccgctgcatc cgacggtcga tggtgactcg atttcgtatc gttttgtctc gctgcgggtg
    29941 gacaagccgc accgcacagc ccacctgacg atgcgcggac ccggcgcggg ccaggacgtg
    30001 cgcaccgccg aggaactgca tgcgctggca agtcgcggcg agctgtgggc gctgcgggca
    30061 tatcgcgaac tcgacgatgc aatctgtcgg ctgcgtttcc actatccgac ggtgggcctg
    30121 gtgatgctgt cgacccgtgg ggatcaggca gaagtgctca aggcggacgc ggcggtggca
    30181 gcgcttcgcg attactggct gggccgggaa gtgctgctgc acatggcgcg ggtgctgcgt
    30241 cggctcgaca tgaccgcgaa gtcgtttttc gccgtggtcg atcccgaaag ctgctttgcc
    30301 ggttcgctgc tggagatcct gctggcgtcg gatcgcagct acgttttgga agatcctggg
    30361 gtgaagtttt cgacgggaac tctcaacggt ggggcgcttt tgtgcggaaa cgggcagacc
    30421 cgtctttcgc tgcgctgtta tgaccagccg gggcgcgcca aagaaatcct cgcacggggc
    30481 gaaaaaggtc cgatctcggc aagcgaggcc gatgcgcttg ggctttgtac ggtgctggcc
    30541 gacgaccttg atttcgaaga cacgctgcga cttgccgtcg aggagcgcgt gagcttgtca
    30601 ccggacgcgc tgacgggcat ggaacagaac ctgcgcttcc ctggccccga tctctcggaa
    30661 ggcaaggtgt ttggtcgtct gtcggcctgg cagaactgga tcttcattcg cgaaaacgcc
    30721 acagggccgc tcggcgcgct gacctgctat ggccgaccgg aacggccaag cttcgactgg
    30781 cgccgcacct agagcgtcac ttccttcccc cgcaggtgta cccgatcggt acacttttct
    30841 cgtcgcagaa ttcaatttcg aagtctagtt ctcacccgcg aagcgtgata agaccgctcc
    30901 atagagtccg agtgcggaat ctcaacctgc agcttcgcta cgaagcgtga cgaaacgaaa
    30961 ggtcagaacc ccgatgaccg tagagctttt cgaaaagatc ccgaacaacg tcaatctgtc
    31021 ggcggacaag cggctccagc gggcgcttga gcagtggctt ccgaactaca tctcatggtg
    31081 gaaagagatg ggtccggacc agttccaaga gagcgatgtc tacctgcgga ccgctatcag
    31141 cgtcgaaacc gacggttggg cgcactttga ctacgtgcgg atgccggaat accgctgggg
    31201 aattttcctc gagccggcga ttgccggtcg cacgatcggt tttggcgatc actacggcca
    31261 gccggcgtgg caggaagttc cgggcgaata ccgcaacacg ttgcgtcgga tcatcgtcac
    31321 gcaaggcgac accgagcccg cgtcggttga gcagcagcgc atgctgggcc acaccgcgcc
    31381 gtctctgtac gatctgcgca acctgttcca ggtcaacgtc gaggaaggcc gtcacttgtg
    31441 ggcgatggtc tatctgcttc actcgtactt tggcaaagac ggtcgcgagg aagccgaagc
    31501 catgctcgag cgccgctcgg gcgatcgtga caagccgcgc atcctcgggg cgttcaatga
    31561 accggtgaag tcgtggctcg acttttacat gttcacgatg tttacggacc gcgacggcaa
    31621 gtttcagctc tgcgcgctga gtgagtcggg atttgatccg ctttcgcgca cctgtaagtt
    31681 catgctgacc gaagaagcgc accacatgtt cgtcggtgaa accggcgtcg atcgcgtggt
    31741 gcagcgcgcc tgtgagctga tgctcgagac gcgctccgat tcgccggacg tgatcaccaa
    31801 ggcgggtggt attccgctcg acatggtgca gcgccacctg aacctgtggt tttcgctgtc
    31861 cgaagacctg ttcggtggcg aaatttcgtc gaacgccgct gagttcttcg ccgccggtct
    31921 caagggccga tacaaagaag acagctacag cgagcacacc gggctaaacc agttctacga
    31981 aatgccagtc cccgccgagg gcggcaagat cgtgacgacc caagtgccgc tgcgcaatgc
    32041 gatgaacgaa gtgctgcgtg atggctatgt caccgactgc cagcgcgccg tcgacaagtg
    32101 gaatcgcacg atcgacaagc agcttggtga gcgtggggct cagttcaagc tcgccctgcc
    32161 gagccgtcgc ttccatcgtc acatcggcat ctacggcatg aactcgggca actacttcga
    32221 cccgaccggc aacccgattt cgcaaggcga gttcatggcc aatcggaaca agtggctgcc
    32281 gaccgacgcc gaccgcgagt atctcaagca gatcatgcgg cctgtgaccg agccggggaa
    32341 aatcgcagcg tggattgcgc cgccggcacg cggcataaac ggtcagaccg tcgagttcga
    32401 gtacgtccgt cacctgccgg cgtaaactgc cagttcttgg gctttcgcct tagccggcgg
    32461 gcttgttgtc ggacgccagt tcttgggctg ccgagtgagc ccgctgcgaa ccaagcggcg
    32521 caatcagcag cccaccgagc gctgccaccg acgccatcgc tccccactgc cacagcaaat
    32581 ttctttccag caacagcccc gccaaaaacg gtgccacgat ctgcgccacc gactggagcg
    32641 actgcgtaag tccaatcacg gtcccttgct ccgatcgatc ggcgctctgg gagatcaggc
    32701 tgctcagcgt gggtcgcagc accgcgttgc caaaggctgt caccgtcgct gcgaccccaa
    32761 gccattgcag tgagttgctc gccgcgatga tgccaaatcc catcaccaaa gacaggtagc
    32821 cggcattgac cagtgatcgc tcgccgaagc gcttgaccag tcgaccgatc agaccgccct
    32881 gaagcaagag cccgaggaag ccgctatagg caaacagata gccgatttcg cgcggaccaa
    32941 acgggtggcc gtgccagacg tagcgccgct cggcgtagag cgaaaaaccc gacgtgaaga
    33001 ccgaaaaaca catcgagtaa aacagaaact gaaacagcat cgctgccaga accggacggt
    33061 caaagtacgc ccgatacgac cgccactcga gaatcgacag ccgcttgcca ccgggaagcg
    33121 ccgcgtcggg ttgctgcgcg cggatcgcca cgccgccggg caaaagcagc agcgtgcaga
    33181 caatcgacac aagcgacatc cccgccgcca ggaaaatagg cgccgccagc ccatgtttta
    33241 caagcacgcc ggtgacgccc ggtccgatga aaaacccgag tccgaacgcc acgccgatca
    33301 ccgcaaagga cttggcgcga tctttggctt cggaatgatc ggcaatgtag gcctgggcaa
    33361 tcgacaggtt tcccgccgtc gtgccttgaa tcacccgcgc cagataaatc atccacagcg
    33421 acgtcgctga cgccatcgcc aggtacccga agagcgcgcc gatttggcta aacagcagca
    33481 ccgatttgcg cccaatccga tcagaccaac tgccgagcag cggacttgcg acgagctgac
    33541 agatcgcgaa caccgaaacg agcagcgttg cttgcagcgg cgtcgcgccc aggtgttcgg
    33601 catagatcgc ttgcagcggc agcaccagcg tcaggccgaa cacatcgaca agcacgatga
    33661 gaaagatggg gatgagtgcg cgattcaatg gaactccgag gcccaaagcg tggcgggccg
    33721 ttctacagac tacttcgatt ggtgggctta ggcgtgcggt cgggatgact ggagcaaaat
    33781 ggcgcgacgc atgcagcgtg cggcctgacg atgctgagca agcgctgagt gcgccaccgc
    33841 gaggtgcagc cagcttacgt ggtgttcggg ctgctgggtg acgacttcgg tcagaagttc
    33901 gaccgcgaca tcaaactgcg ccgcgctgag cgccgttacc gccggcagca gcttgtcgtg
    33961 atgcgtcggc tttgcgcgtc cttccgaagt tgccaaacga cgacggacgg gcagggcatc
    34021 cagcaggctg cgaggtgacg acggacgcgg catcttggct ttgccactgc gccgcaccgc
    34081 gtggggtttg ggcagcggat tttgggcggt cttgcccagc aaggcgcctt cgcgcgtcgg
    34141 acgggtcagt gggatcagct cgacagcaac cgcgtgtggg gtgcgcgcat cgagcatgac
    34201 catttcgagc agcgccgtgc cgatctcgcc gccgtggcgc accacttcgc gcatcaccga
    34261 cgccagttca tccggcggca ccgatccgag aagatcatcg accgtgcgcc gcagatcgtc
    34321 cgcgcaagca atcgtcgcca gaagctgcgc caccgaacgc gcaataaaac cgtcggggtc
    34381 ttggatcgcc acgtccaccg ggggcgacac gcccaagtcc gacatcaccg acagcgcacg
    34441 cgctttcacg gtgtcgcttt gcgtgctttg acacagcagc ttgcgcagtc gcgtcgtcac
    34501 atcggtcaca tcggcgcgcg ccagaagctg catcgcccac tgggcttcgc tgtcgctgtt
    34561 cgacgcgagc gagcgcagaa gctgtggcaa aaggacgctg cccaaacgag gcaccagatg
    34621 caaaagccgt tgcttgtgct cgccagtcgg gagttcgccg tcacgggccc tacgcagcga
    34681 cgccgctacc tccaaaagag gggtgagcgc cacggcttgg tcttcgcgtt ctgtcacgga
    34741 ctgggccaca ccggcaaacg tcatgctggc tcctaacgtg aaacgaccga cttgcaccaa
    34801 ggtacatgac atatatggtc attcttcgtc aacgattccc tggacttgcg tgccgggtgg
    34861 ggcttggttt tcgtcggatt gcaatttttc ggcttgacag gtgggcgcct atgctagttt
    34921 gcccgacatg atggccgaga gttttcacaa aaaggcgctg gctcgtgagc tccgcccggc
    34981 gctatcgaag gatgaggcgc tcaccgaggc caatcggtgc ttgttttgct acgacgcgcc
    35041 gtgcatgcgg gcgtgtccga cgcacatcga tgtgccgctg tttatcagac agatttcgac
    35101 ggcaaatccg acgggtgcgg cgcgcacgat tttggccgcc aacatccttg gagcaagctg
    35161 cgcgcgggtt tgtccgacgg aagtgctgtg cgaaggcgcc tgcgtgcttt ccgaccagca
    35221 caaaccgatt cagattggac cgctacagcg ctacgcgacc gatcatgcga tgcagacggg
    35281 aatgccgatt ctcaaaccag ggaaaaagca gcctggatcg gttggaatca tcggggctgg
    35341 accgtcagga ctggcctgtg cggcagagct gctcaagctt ggctacgaat cggtgattta
    35401 tgaagcccac gaccggcccg gcgggctcaa ttcggtcggg gttgcgcagt ataaaatgac
    35461 gccggatgtg gccctggccg aagtcgactg gcttgtgcga tccggggtgc agatccgctg
    35521 cggtgtcacc gtcggaaaag acgtaagttt tgccgaattg gaaagccgcc acgacgcgat
    35581 gtttatcggc gtcggcatgg gcggaattcc gggcatcggc gtggctgggg aagacatttc
    35641 cggcgtgtgg gatggcctgg atttcatcgc ggcgctcaaa cagggcgaca gcgaggtgtt
    35701 cgatgcgcta cgtggcgcgc gtgtcgcggt cattggcggc ggcaacaccg cgattgacgt
    35761 cgtgacgcag gcggcccggg ccggcgcgtc gaaagtgtgg cttctgtatc gtcgtggtcg
    35821 cgagcagatg tcggcctatg cgcacgagat cgagctggcc aagcagcatg gcatcgagct
    35881 tctgctgcat gccgtgccgc atcgcgtgct tggtccgtcg cggggcctgt caccgcgcag
    35941 cagccgcgtc gctggactgg agtttggtcg caagcttccc gacggtggtg aaagactgga
    36001 aaccctgcct gccgacctgg tgattcgggc gaccggacaa aaaggggcca agctgaccgc
    36061 ggaactgccg gtgcgtacag acggtggaaa agtgctggct gacgagtcgg ggcggacttc
    36121 gaatccgcgc tacttcgcgg gtggtgactg cgtgtcgggc ggccaagaag tggtcaacgc
    36181 cgtggctgaa ggccagcggg cagcgcggac gattgtcgct gaagtgctgt ctttgcgtgg
    36241 aggggcgcgg tagcgccgga ggagttcgcg atggccgatc tttccgtcga tttttgtgga
    36301 atcaagtcgc ccaatccgtt ctggttggct tcggcgccac cgacgaacac aggcgatcaa
    36361 atcatgcgtg cctttgacgc tggctggggc ggagcggtgt ggaagacgct cgggcagcca
    36421 atcgtcaacg tgtcgtcgcg ctattcatcg gtcgactatg ccggacgacg catgatgggg
    36481 cttaacaaca ttgagctcat caccgatcga ccgctcgacg tgaacttccg cgaagtcagc
    36541 gaggtcaaaa agcgctatcc caaaaacgcg ctgatcgtgt cgctgatggt cgagtcgaag
    36601 cgcgaagcct ggcacgaaat cgtccgtcgt gccgaagacg ctggtgccga tggcctggag
    36661 ctaaacttcg gctgtccgca cgggatgagc gagcgcggca tgggcagcgc cgtcggacag
    36721 gtgcccgact acacctgcca aatcgtcaag tgggtcaaag aagtcgccaa gacgccggtg
    36781 attgtgaagc tgacgccgaa catcaccgac atcacgttcc cggcgcgcgc ggcagagcag
    36841 ggtggggccg atgcggtgtc gctcatcaac acgatcaact cggtgacggg aattgatctg
    36901 gaaacgatga cgccgcgtcc ggcggtccat ggtgcatcgg cgcacggcgg ctactgcggt
    36961 cccgcggtca aaccgatagc gctgtcgatg gtcgcgtccg ttggccagac ggtgaagatc
    37021 ccgatatctg gcatcggtgg cattgccaac tggcacgacg cggcagagtt cattctgatg
    37081 ggcgcgacga cggtgcaggt ctgtactgcg gtcatgcacc acggcttccg catcgtgctc
    37141 gacatgattg acggtctgtc gtcgtatctg gaccgcaaag ggctggccag cgtaagtgac
    37201 ttggtgggcc gcagcttgcc gacctatcag cgctgggaac agctggacct aaactaccgc
    37261 gtcatcgccg acatcaagca ggacaagtgc atcggctgtg gcgtctgtta tgccgcttgc
    37321 tacgacggag cgcaccaggc catcgctgcc aaaccggatg ggcggggtcg ttcggcggtg
    37381 caagtcctag aacacaagtg cgtcgggtgc aacctctgta gcttggtctg ccccgtcgag
    37441 gattgcatca cgatgaagcg ggtcgacacg ggttctgaca cgcgcgtgcc gtggacggaa
    37501 tacagcaaga acccgtcttt gtatccgcac atccgatcca atcgtcacga ccagtaagcc
    37561 atgttgccgt cgcttgtgac cgtggcgcag ggcctttatg ggcttcttct cctcggactg
    37621 tggctgcttt tgatcgcggc gagcgcgcat cttttgcggc ttggactgtg ggcgcttgcg
    37681 catccgctgg cttctgtgtc ggttcggaag ctggctgatg gtgatttgcc ggttgtgacc
    37741 gtgcagctac cgatgcgaaa tgaacgactg gtggcggcgc gggcgattcg ggctgcgtgt
    37801 gcgctcgact ggcccaaaga tcggctgcaa attcaggtgc tcgatgacag cgatgcaacc
    37861 gacgaaacgg tggcgattgt cgatgcggaa gtggcggcgc tgcgatatgc cggatttgac
    37921 atcagcgcgg tgcgtcgtcc cgaccgacgg agcttcaagg cgggccatct ggactttgcg
    37981 ctgccgtctt cgcgcggtga attcattgcc gtgctcgatg tcgattttgt gccccaagcg
    38041 gattttctgc gacagctcgt gccgcatttg gttgcgcaac cgcagcttgc gtttgtgcag
    38101 ggtcgctggt cgttcctcaa cgaagacgac agcctgcttt tgcggctcca agcgctgatc
    38161 ctgcatggtc ttttcctcgt cgagcagtcc tatctttcag cgcaccggca gccggttcag
    38221 ttcaacgggt cgggcggaat ttggcgcaaa gcggcgcttt cgcaagcggg cggctgggtc
    38281 ggtggcgaca gcgataccga agcgtcggtg accgaagacc tcgatctgtc gtttcgcgtg
    38341 cgactgtttg ggtatgaatc gctgcttttg ccgcaggttg cggtgcccac ggagcttccg
    38401 gaatcgatgg cggcgtttcg cagccagcag aagcgctggg tgcgcggtgg ggcccaggtg
    38461 ctgcgcagtc tgctgcacaa gctgtggtcg ccggggcgtt cgcttggcga gcggctgacg
    38521 atgctgctcc atctgttgcg gcatgcgcgg cagccgtatc tggcgctggc gcttttgttt
    38581 ttgccgctgc caccgcttgg cgtgctgcat ggtctgtttg cgccaccggg aggcatgctc
    38641 gcagcggtgc tgatcctgtg gacggcgctt tcggtgtatt acggggcggc gctcttgcga
    38701 ctgcagcgct cgccgttttg ggcggtgctg ctggccccgc ttgtgatggc actgtcgatg
    38761 ggcttgtgtg tatcgctcac cgtcgagctt ttgcgtggcg tgctgtcctc gcgcaccggc
    38821 gcggagtttg ttcgcacgcc gaagacggga ggacagaaag aacctgccaa gcagtaccga
    38881 cccgtgtttg ataggctcgc gcggatcgaa gtcgggatcg gcggggcgta tctggcgctg
    38941 tcgttttggc tgttttggcg cggcgactac ctgaccgcgc tcggctttgg gctgctgatt
    39001 ggggccgggc ttctgtgggt cggcggcggt agctggcgca tttcgcgcta gcggttacgc
    39061 ggagctcgcc gacggagccg ccggggcagc gcccggatca ggcagggcag atgcggcatg
    39121 accgagagcc actttggcgc gcttgccaaa cggtgtctgc ggataggctg cggctcgttc
    39181 gtagcgacga cgggcttcca cgggcttgtt ttcagcttgt gccacttcgc ccagccaaaa
    39241 ccagcgctcg gcggtgcggc ccggatcgac ctgccattcg ggatcgaact ggagcgacac
    39301 cgactcttct tcggaacgca gcgccgacaa aagcgccgct tccgatgctt ggtagttttt
    39361 gcgatgcagc tcggcaatgc cgcgggtgcc actgatacag gctgacaaaa gcggatcttt
    39421 cgatggtcca tcgtcgcccg ccagcagctc gaccgcatca tccaggtgtc cgaccgcatc
    39481 atcgggcttg cccagaaatg tcagcgtata cgcccggttg ttgagccaca gcgcgacttc
    39541 gaccgggctt agctgctgaa gctgcacttt gtcgagctcc tccagtgctt cgcggtgatc
    39601 gctgacagca ttgagcgcac acgccaggtt gatgcgcaaa agctgttctt cgcgcagtgg
    39661 catcgtgcgg gcaagcgcga cgcggtagta ccggatgagc agcgccgggt cgtcgctcgg
    39721 caggctgggc ggatcgtcgt cggcatgctc gctgagcagc agcgccgagc gattgcgctc
    39781 gtggctggtc agcgccgcga tgacggtcac ccagacaagc aggaagaaga ttcccagcac
    39841 gactctagtt tactccgctt aggtgccctc gctccgacga tcttgtcacg aaatgccgag
    39901 cctgatgctg cgggcaagca gcgccgacaa gacggtttcc agcgtcgaga caagctcggc
    39961 atcggcggtc ttccacagct tgccgtccgc atccggatcg aagtgccacg ccttgcgctg
    40021 cgccgccatc cagatttgcc gagccgcccg gtgccggttg atgacaattt tttgcccgtt
    40081 gccgagcgtc agattcagca cgtcgccgga catctcgacg tcgacttcat cgggatcaaa
    40141 ggtggaaacc gctcgctcga tgcgacggat catcgcgtcg gcgagcttgt caaactctgc
    40201 gtcctgtttt tcgcgcggat tcatagcgtt ccgtgagcct agcgcgcctt gcgctttccg
    40261 atagatgaaa tgtcaccaaa gcgccagctg cgtcacctgc tgtggcctac tggcggagag
    40321 cgatgggaac ttttttgccc aattgatcga tgactttttg gtgggctttc tggacttcgt
    40381 catcggtcag cgtccgctgg tctgattgat aggtcaggct aaacagcagc gctttctgtc
    40441 ccgccggaac gtgtccttgg gcgcgatagt cttcgagcag tgccacatca cggagcagcg
    40501 gctcttccga cttttgcatc gcatcgagaa gcgctgccgc ggacacatcc tgcgcaacga
    40561 agaacgacag atcacggctc gtcgctggga atcgcgagtg tgccacgtag ttgggcttgc
    40621 tgattggcag gtccggcacc gcaagttcca gcgcaaacgc tgccccaggc agatccagct
    40681 tgctgcgcag atccggatgc agttcaccaa acgtcccgat gaccgcaccg ctgtgcgcaa
    40741 aacacagtgc cgctgccgca ccaggatgca gcatcggggc ttcgtctgcc gtcgctgcgc
    40801 gcacaaacac ggcacgcgcg ccggcacttg ggtcaggctg ccacacaagc gaaagaccga
    40861 gccctgcgac aagctgctcg accaggcctt ttgcatcgta aaagtcgagc gcatcgcccg
    40921 gtgtcggttt gagaaagtga tcgcgatgac cgcagagcac cgccgcaacg tgggtttgtt
    40981 ccgtcggcag gacttcgttg gcaccggacg gctgaaacac ttcgcccact tcaaacagcc
    41041 gcaggtcggt ctgtccgtgc gaaaggtttt gccgcacaag ctcgaccaaa ccgggtagga
    41101 gcttgctccg cagcaccgaa agttcttcgc gcagcgggtt ttcgatgcaa agcggtgcaa
    41161 gctcgcgccg accttcgccg agctttaccc gatcgggact cgtcatcgaa aactgaatca
    41221 cttcgagcag cccaagcgac gcacagatca ttcgtgcgcg gtcggcattg cggtagcgcg
    41281 tcatctgcac agacggcggc tgtggctcgc tcatcggcat gttcggcagc gtggtcggaa
    41341 tgtggtgcag accgtagacg cgcgcgactt cttcgatcag atcaatttcg cgggtcaaat
    41401 cggggcggcg ggtcggaatc tcgaccgtca gaagtttccc gtcgctgtgt acgggcaaac
    41461 cgatcgcttc cagacactcc ttttgcatcg ctgcggaaat cgtcagcccc agcatggccg
    41521 tcgtgcgctc cgggcgcaac gtcagcgtcg gcaacgacag ttttttcggg tacacatcgg
    41581 tcgccgcagc agccagttgt ccacccgcga gcttcacaat cagtgcggcg caggcatcgg
    41641 cgacttcggg aattgcattg gcatcggtgc cacgctcaaa gcgatgcgac gcttcggtgt
    41701 gcagcttcag ctttttcgcg gtgcgacgaa tcatcgccgg tgcaaagtac gcgcactcca
    41761 gcagcacatc ggtcgtcgat tcaccgactt ccgaagtctg tccgcccatc acacccgcta
    41821 cggcgacgcc cttttcggca tcggcaatca gcagatcaga tggacccact tcgcgctctt
    41881 gtccgtcgag cgttttgatc ttttcttctg gcttggcccg tcgcacgaca attttctgtc
    41941 ccgcgagctt gtgcagatca aatgcatgga gcggatgccc atactgaagc agcgccaggt
    42001 tggtcgcatc gacgacattc gacagtgctc gcaccccgag tcgctggagt cgcagacgat
    42061 aaaacagcgg acttttgccg actttcacgc cgcggatgag gtggcccaga tagcgcgggc
    42121 aagactcgct gtcttcgacg acaatttgcg ctggttcggt ttgtgcctga ttgccgcgaa
    42181 gtgccgccag attgaccgac tgctgccgaa gtcgcacgcc ttcgtgacgg aacagcgccg
    42241 caatttcacg cgcgacgcct gtgtgaccca aaaggtctgg ccgattgggc gtaatgtcca
    42301 gctcaaacac ccaatcaggc agtccggcgg cttgcgcaac atcgccgcca atgtcgatgc
    42361 cgtcatcggg cgacagaatc acaatcccgt cgtggtcttc ggaaagcccc agctcgtctt
    42421 cggcacacag catgccgggc gacggaatgc cgcgcacttc gcgaacgctc aggacaagtc
    42481 cgtttggcag cgtcgcgccg ggctttgccc acagcacccg cggactgcgc cccggctcac
    42541 cggggtttgg cacattgggc gctccgcaga cgacctgcgt gacttctttt ccatcaaaga
    42601 catcgaccag cgtgagctta tcggccttgg gatgtggacg cttgcctcgc acttctgcgc
    42661 aaatgacgcc cgaaagacct ttgccaatcg gatgcagcgc atcgaccgcc acgccggaca
    42721 cgctcagtcg gtcggcgacc gcttgcgggg tcacgctcga cggaatctcc agtagctcac
    42781 agagccagtt ccaagagact ttcattgcca aactcctcgc ctaacgaaac tgctcggtga
    42841 agcgggcatc ccccgcgaaa aacagacgca gatcgctaat gccatagcgc agcatcgtca
    42901 tgcgttccag gccggtgcca aacgcaaagc ccgaatactt ttcgctgtcg taaccgacgt
    42961 ggcggaagac ttcgggatcg accatgccgc agcccaggat ttcgatataa cccgaacctt
    43021 tgcaggtgcg gcagcccgag cgcttgcaga aaaagcactc gatgtcgact tcggcgctcg
    43081 gttcggtaaa cgggaagaac gacggacgca gtcgcaggcc aatccccgga ccaaagaagc
    43141 gctgcacaaa ataaagcagc gtgcctttta agtcggccat tgtgatgcct tcgtcgacgc
    43201 acagcccttc gatttgctgg aacatcggcg aatgcgtcgg gtcgtcgtct ttgcgataga
    43261 ctttgcccgg cgagatgatg cgcacgggcg gtttctgcgc aagcatggtt cggatttgca
    43321 ctggcgaggt gtgcgtgcgc agcaccagcg gttcgtgccc cggcttgctt tgcagataaa
    43381 acgtatcctg catatcgcgc gcgggatggt ctttgggcat cgccagcgct tcaaagttgt
    43441 gaaaatcggt ctcgacctgc ggacccgtgg cgacgtgaaa gcccagctcg gcgaagatgc
    43501 cttccagttc cagccgcact tgggtcagca gatgcaagcc gcccagcttg tgctgccgac
    43561 ccggaatcgt cacatcgagc cgccgctgga gatcggcttg gcgcttttgc tcgtgcagct
    43621 ccgcgagccg ggccgcaaac agttcctcgg ctttttgcag tgcaacattt ccgaatcgac
    43681 cgatctcagc tttcgggtgc gtgtacggat ggtttgtggg tgcggcaccc ctcggaaagt
    43741 tgggattctg ctccgcgcgt ttttccggcc cgggggcggc ttttagcgca tcggcgagac
    43801 gcttgcgaat ctcgccttct ttgccggcaa agcgcgcata aacgacccgc agcgcaggtt
    43861 cgtctttggc caaggccagc tctttttcaa aacgagaaag ggattcatcg aagaggcggg
    43921 ccagctcgcc cgcgcggctg tcattttggt tcatcgcgat accttacccc agaaatgaca
    43981 atgcccgccg aagcgggcat gtgtgacaga caagatgggc caggacttgt gcgttacgcc
    44041 tgagccttga ttcctgcctg cgcggcaatt gctttaaagc cagccggatc gctgatcgcg
    44101 agatccgaga ggatcttgcg gtcgatcttg atgttggcct gcgcaagcgc gtggatcagc
    44161 ttcgagtagg tggtgccaat ctcacgggct gccgcattga tccggatgat ccacaggcgg
    44221 cggaaatcac gcttgcgctc gcgacggtgc caggtggcaa atcgccacgc atgacgcacc
    44281 gtttcgacca tcgctttcca cagtgtggac cgacctccgc ggtagccttg ggcaaaggcc
    44341 ttgatgcggt tgcgacggcg acgtgcttta aaacctcgtt ttgctcttgc catgactctt
    44401 tgtcttcctc agtcgtatcg gcatcggggc tgcttgaaca aaagcagctt ggaaccctga
    44461 gcctgatgtt agaagggccg acccgaggcc gacccatacg agaggccact tggtaacaca
    44521 gtggccctcg atttgtaaag tccgatgagg acttcggaaa cggctgcttg ggccgcgcta
    44581 cttctttttg ccggggccgt tgtggtactt catcaggcgc ttttcgccga tggtgtggat
    44641 cttgttgtcg gcgaccagct caccgagcgg ctggcgcagc ttgttggcca gttccgcacg
    44701 atcgacaccc acgttggcga gcagctcgtt gttgatgttg tcggcgattt cgccacggct
    44761 caggccatct ttggcattcg agagcacgct ggtgatgatg cctttgagcg agttcttctg
    44821 cgcttccgac aggcgggtgc gcttggctgc ggccttgccc ggcttgcttt ccgtcggacg
    44881 cttgccaccg atcgcttcca caaagtcaat gatgcccatg gtcgacactg ccgcccagac
    44941 atccttgtgc tgcttgaggc tggtgatgaa ctcctcgacc gtcgtcttgt tggtcgggtg
    45001 cagtttgaag aacatcgtca tcgcagcttg gcgacgcaga tcctcgttac gcgcctgaag
    45061 ctcttgctca aacagggcgg taatgtcaaa agcggttcca gtcttttttc cagccattgt
    45121 gatctcctat gtgattgcca gatgtcctgg cctggtttga ccaacgtttt cttctactag
    45181 tacaacttga tacgcaattt gcaaccggtt tgtggtctgc gaaacgcggg cgcattcaaa
    45241 aactgatgtg acttgctgtc aagcgccaaa atctgaccct ccagtcgatg ccatctgttg
    45301 gaagcagact gtggtaggat gcctcctcct atgaagattc gccgccagct ctcaccgctc
    45361 tacttaagtt ttccgctggt cttgatcgcg gcgctggcgc tggctcgcta ctcgtatcgc
    45421 aacgcccgac aacttgcgca gaacagcgaa ggatcggtga ttgcaggttt gttcgcactt
    45481 ggtgatcaga cccgcagtcg cgtcgagagc ttcatcattg actccgaccg cgagctattt
    45541 aatctcgtcg acctcgaaca cctgcaggac ttttcgcgtc gctggtcgga aattgttcgc
    45601 ttatcgcatg cagtcgaagc cgcgattgtg ctcgatgaaa accagcagct cgtgcgcggc
    45661 ggctatgtca ataagcgacg caacaagccc gaggccgaag cgtttctgcg tctgttcatg
    45721 gatcgcattc gacccgagct gccgctcgcc gagcagcgcc ccgacttcca ccgccatctg
    45781 cacaccaatg tggatggtgt cgattacctg ctcagttaca tcaagcgcta tgacgccgcc
    45841 cgcaatcgcg tcttttacat catcctgaag gtgaacctcg actacttggt gggcacgagc
    45901 ttccccgaga tgttcgagcc gatcgccggt cgcttcctgg tttcggtgat tggcagtcgc
    45961 ggcgaagtga tctttggtca gccgatctcg ggggcgggca aatacctgta cgacaagcgg
    46021 tttgaatcga ccgtctatca gtggcgcttg cagatggcgc cgctcgataa cgtcaagctg
    46081 cgcgctggga gccaaaaaca agcactcgca gataccattt tgattggtct gatgcttgga
    46141 ctgattctga ttggtacggc cttccttgtg tactcgatct caaccgagca aaggctatcg
    46201 gcgctcaagt cggaatttat ctccaatgtc agtcatgaac taaagacgcc gctgtcgctg
    46261 attcggatgt ttgctgagct gcttatgctt ggcaaagtga agacgccgga aaaaggccgt
    46321 gagtacgcgg gaatcatcac ccgtgagtcc gagcggctgt cgcgacttat cgacaacgtg
    46381 ctcgactttt cgcggatgga gcgtggcaag gcggcctacg agatgaagcc cggtgatctg
    46441 ggtgaagtgg tagaacgggc gctggatttt ttccgctatc gccttgagcg cgaagggcgg
    46501 cagcttgaag tcgacatcgc agagtccata ccgcgcacgc tgctggatga aaatgcgatg
    46561 accctgttgc tgctcaatct ggtggataat gcggtcaaat atggcggaac cggaccgatc
    46621 gcggtaaggc tttgtgtctc gccgcgcggc aaggagattc ttctgagcgt gaccgaccac
    46681 ggactgggga ttgcccccga cgagcagcgc aaaatcttcg agcgctttta tcgaacccgc
    46741 gcagtccgca acaccaacat tcgcggatcg ggcatcggtc tggcgctcgt caaacacatc
    46801 gcggaagcgc atggtggcca catcactgtc gacagccaag gcggcaaagg atcgacattt
    46861 acgctgacgc tgccggtttc ggcgatcaaa gaagaaccag acgaagcggg tggcgacgcc
    46921 gcttttgaca agggagacag ctagatggca ctgccgtcat ctggaccggc cagcgccagc
    46981 agcccaccgc agagtggctc tggcgagctt agcaagcgcg cccggatcct gcttgtcgag
    47041 gatgagcccg acattgtccg tggtctcacc gatgtccttg agtttgaagg ctacgaggtc
    47101 gtcacccaca tgcagggtcg cgcgggcgtg cgcgaaatga aggagcgtgg tgccgacctc
    47161 gtgatcctcg acctgatgct tccggacatc aacggattcc aagtctgcga ggagatccgt
    47221 gccgaaaacc aagtggtgcc gatcatcatc ctgacggcac gcggccaaga actggacaaa
    47281 attcgtggtc tggaagcggg cgctgacgac tacgtgacca agccatttgg cgtcggtgag
    47341 ctgcttgcgc ggatcaaagc catcctgcgc cgcctgaccc gcgcccaagt cagcaaagag
    47401 cccgaaacca tccgcatcgg tagctgcgag atcaacctgc ggacccacac ccttgtgcgc
    47461 gcccgcaaaa gccacatgct cacgttctac gaagtcgagc tgctgaagct cctgtacgag
    47521 cgcgaggggc aaccggtttc acgcgacgag attctcgaca aaatttgggg catcgaagcg
    47581 tatccgtcga accggacggt ggacaacttc gtcgtcaagc tgcgcaagaa gctcgaagaa
    47641 aaccagcacc acccgcgcca catcctgacg gtctacggct tcggctacaa actggttccc
    47701 tagcctcggc ttggtctgcc ttcgttccaa ctgggggaat ccctgcgcgt gccttcggga
    47761 aactgtgggg tgctgcggtg ggatagcgtg ccgcgatcga agcgcagata gatcgtcaaa
    47821 aacagcatga cgccgtaagt tagattgagg tgcagccgtc gaccagccgg ccagacgact
    47881 tgcagcagca ccgaaaaaag gcacagcgcc acgatccatc cgtaccgacg tccaacgtgc
    47941 ggcatctcgc ttgcgaacag tgcgcccagg cccaccgttt cttgaatcaa aaaacgatcg
    48001 gcgggaatgt cgagccacac accaatcgat cgtccgaata ccaaatagga catcgtggcg
    48061 agcatgaagt agccaagtcg ccgataaccg ggctgctggt gaatctgctg gcggaatcca
    48121 gccacgctga gcgatgtaac agagagcagc gctgttgcga tgagcagcgc gggtcgcaga
    48181 tcgctgcgat gcactccgcc cagcgcgagc agcacgccca gcacaatcac cgaaaagctg
    48241 ctcagcatga cgtgcaggaa gcggcgtttg ttttgccgca tgctttccga atcggcatcc
    48301 ggcagcaacc ccgcttgcag cgcttcggtc agctcttgca tcgacggaaa gcgctccatc
    48361 ggatcgatgg acagaccacg catgagcgcg gcgtggatca caagcggcac ttcgtagcca
    48421 tgcagcgttt tggtgcgcgt gctgggcttg ccgctgcgga tgctggcggc gtattcctct
    48481 agcgtggtgc cggcaaatgc ggggaatccg tagatggctt caaacagtgc tgcgcagaat
    48541 ccgaactgat cactgcgcgc atcggcctgc tctccgcgta cttgctcggg agccatatag
    48601 ccgggcgtgc ccaggatggc accaaactgc gtcagtcttt cgctggtggt tcgcagcttg
    48661 gcttggcttg cttcaggtga ttcattcttg tgggaatggg aatcatcagg ggctctggca
    48721 atgccaaagt ccatcacccg cgcccgccca tcgctgccca cgatcacgtt gtcaggttta
    48781 aaatctcggt ggatgagacc ggaattgtgg gctgcggaca gaccggctgc ggcttgcaag
    48841 taggtgcgca gacagtggtc gagcgaagtc gcatcgcgga ccggatgctg attttgccac
    48901 tcgacgagat cgctgccggg gacaaactcc atagcgatga agatttgtcc ctgcggcgac
    48961 tcgcgatctt ccccgacttc gtagacgtgc accacgttgg gatgggcaat ccgcgccatc
    49021 gccttggctt cttttagcag ccgctcgcgt gcgatttgac cccggtgtac gtcgtctcgc
    49081 acgaccttca ctgcaacctt gcgatccaga tcgggatcgt aggccgcata cacggttccc
    49141 attccgcctt gtcccaggca acgcacgacc atgtagcgac cgatccgcac cgggtcgaga
    49201 agctgaggat tgacgacgat cggcacgatg gcctgcgctc cggttgacga tccgggcgcc
    49261 ttgatcgctg tctcggcgcg ggaaatgggc ggcgactcta ctggcgtcgc ggcggttcga
    49321 cggtctgcct ggcgatcgat ggtcttggcc gatagatcaa cgagctgatg cggcgtcgac
    49381 tcgatggtcg cgctttctcg ctgtggcgtc ccgtcgccag gctctcggcg gagattttcc
    49441 acgatcggca catccagatc gtaccatgca acgacagcag ttttccgacg gaaaagtggg
    49501 tatgcacgac gagcaggttg ggggcgtgcc gacgccagcc cggacaccta tccggcttgc
    49561 ggtacggcgc gccgacatac cctgcgatgg cttggcatgt caccacggga agactgcaag
    49621 tggtgccgag caacacccag cgtttgcgcg cgaacgttga gcgtcgtcca gcaccacttg
    49681 cggagtccct gcggatactt tgccagtcga ccactggtac ttcagccttg gtttttatcc
    49741 gatgacaggt gccgccgcgt actttgcatg tagttcctag tacttcgggc ctgcggggac
    49801 catcgggcgg gtgtcccacg ggctcggagt acggacaggc ttttggactt tgtctttgtc
    49861 tgcacgcgcc actttgtcgc tcttgacgtc ttctttgtca gacgccgacg cgggagcaaa
    49921 cggaagaagg aggcaagacg ttacgccgac aagaatgcca accagcaact gcttgaatga
    49981 catggcgaac ctccgcgaac caggttcgtt tgttcagagc gagcggcccc atgccgatgc
    50041 tcaaaccaaa cgaccgccga ttcaaaagtc caaaggacct atccggtacc tttagtgcgc
    50101 acatttgtac ggcttgtggg cggtgtcaag ggtcattttt caaacatcgc aacttttttt
    50161 ctgccccgtc gcccagatca ccacaaactc gcagaaggtc ggaccgccat ccagacttcg
    50221 cagcagccgg cccgctgtgt taggttgccc cgcttggaag tctgatgcgc attgccaacc
    50281 ttgtcgtcga taccgaagtt ctgctggctc cgatggcggc ggtcactgat ctgccgtttc
    50341 gcaccatctg cgaggagcat ggcgtgggtc tgaccatcac ggagtttctg tcggcccggg
    50401 cgctgtgtga aggtgctaag aagacgacgc agaagcttac ggcatcgctt ggtggacggc
    50461 gtttcggcgt gcagattttt gggcgtgaac ccgatgcact ggccaaggcg gcgcagcttg
    50521 ccaaagagat tggggcttcg ctggtcgaca tcaacatggg ctgtccggcc aagcgggtgg
    50581 tcgcgggcgc ctgtgggtcc gctctcatga aagagccaga gcttgcgcaa gtgctggtgc
    50641 gggctgtccg ttctgcactg ccggctgaca ttccggtcac ggtgaagcac cgcgctggct
    50701 gggatgaaaa acacctcaac gcgccagagt ttgcctgcgc catggtggaa gccggggcgg
    50761 cgatgatcac ggtgcatgga cggacccgga cccagggttt ttctggcaag tcctcgctcg
    50821 acatcattcg caaagtacgg gacgcggtgc cgcgccatgt gccggtggtg ggcaatggcg
    50881 atgtgatcac gctgctcgac tacgtgcgga tgcgaacaga gacgggctgc gacgcggtga
    50941 tgatcggacg cggtgcgctt ggcaacccct ggctgtttcg tgacatcggt gcgtggctgc
    51001 gtggccagcc ggtgccaggg gcgcccacgg tggaggagcg cagcgaggtg tttttgcgcc
    51061 acgttgcgct catgcgcgga cggaccgaag acaaacgact gatccatgag gtgcgcaaag
    51121 ccgtcgcttg gtacacaaaa ggactgcctg gctctgccga gctgcgcgat caacacaaag
    51181 ccccgcgcga accagacgcc ttggtcgccg cgacggttgc atattttacg gagctggcca
    51241 aaggcaggcc gcggctcaaa gttgcgctgg atgcatccgc ggctcaagcg ggcaccgatg
    51301 aaaccaccgc aaaggcggtc tgtgcatgaa agcggcaagt ctggctccct gcatcgcgct
    51361 gttgggcgaa gcgccggtga cgctgcgcct gcgggctctt gtcgaacagg ctgcgcaggt
    51421 cgaaaccccg ctgtttgtcg aaggtgagcg cggatcgggg cgcgagcaca tcggtcgtta
    51481 catccactcg acatccccgc ggcgcagcgc ggcgttcatt cggatcgacg ccgaatatgc
    51541 cgacaccgag cgggcgattg gcaagctccg acgatcgcac ggcggaacgc tgctggtaaa
    51601 agacgtgcct tatgggccgc tgcggctgcg caaagtgctg caagggctgc tggagcggca
    51661 ggaactggaa tccggtcagc tacgcagcga aggcgagggc gcagagctgc cgcggctgat
    51721 tctgacaagt gacgtggaac tgccacggct gcgtgagtcg ggctttgcgc tggcggaact
    51781 tttgcttttg catcaggtga cgcggctgcg gattccgccc ctgcgtgagc gggtttctga
    51841 cgtgccgctc ctggctgaac acttcttgca gcttctgggc gaacagatgg ggcgtcgaca
    51901 caagctgtgc agccgcgcgg tcgatcagct catccgctat agctggcccg gcaacttggc
    51961 ggagctgcgc gatgtgatca gtcgttcggc gctgcgcagc cggggcgaaa ccatcgaact
    52021 tggcgaagtc gaagcggtgc tgccaccgct gcaagagcgt gtaccgatcg aacagctgtc
    52081 gctagaggaa accgtgcggg ccaagctacg ggcgcttttg tcgcggctgg aaggctgccc
    52141 gctccacgac ttgaacctgt acgaagaggt gctgggtcac gtcgagcggc ccctgctaaa
    52201 agaagtgcta cagcggaccg aaggcaatca ggtgcgcgcg gcggccattt tgggaatcaa
    52261 tcgcaacacg ctgcgcaaaa agctgcgcga gcgcggcctg ggcagcgagt tcttggacac
    52321 accgacggat tcgcctttgc gccatctgcc gtaagcaact gccaacgctc ggcagttgac
    52381 gaaatgcgtg cggaagcgga atctcaccgc ctacggagaa cttcacccat ggcgcttagt
    52441 cctgacactc gctcgcaaat cgagacgctg ctgcggcagc acaccatcgt tctgtttatg
    52501 aaggggagcc ggcagatgcc gcgctgtggc ttttccgcca aggtcatcga gatcctggaa
    52561 ggccatggca tcgactatcg ggatgtcgac gtccttagcg acccggcgct gcgcgaaggc
    52621 atcaaagagt tttccaactg gccgacgata ccgcagctgt ttttccaggg cagcttgatc
    52681 ggcggcagcg acatcgtgcg tcagctcgac gaaagcggag aactgctcca ggcattgggc
    52741 attgacccga gcaaggtttc gatcgagccg ccgcgcatct cgattacgcc tgaagctgcc
    52801 gctgccatca aagacgccgg caaagaagcc gaacccggac agcacctgcg catggtgctt
    52861 agccgcggcg gacagaacat cgacctgtac ttcgatgccg ccaaagccag cgatctgcgc
    52921 gtcgagcagc acggcatcac ggtgctgttt gaccgggctt cggcgcggct cgcgagtggc
    52981 atttcgatag cgtatgtgga tggaccgggc ggtggtttcc gcatcgacaa tccgaacctg
    53041 cccgcgcagg tgggcacgct gacgccggca gagctgcgca agatgatgga tgccaaagtg
    53101 ccgctgcggc tcatcgacgt gcggaccgag cgtgagcggg cgattgcgcg cattttgccg
    53161 gatgtgacgc tggatcagat tgcgccggag ctggacagcc tgccaaaaga cacgatgctg
    53221 gtgttttact gccattcggg cgtgcgcagt cgggcagcag cggaacaggc gcttgcgcgt
    53281 ggcttccgca acgtctggaa cctaaatgga ggcatcgacg gctggtctcg ccaggtcgat
    53341 aaagacgtgc cccgttacta agcgaaagcc aacaccgtga agggcattat cctcgccggg
    53401 ggaagtggga cgcggcttta tccactgacc aagacgctga gcaaacagct tctgccggtc
    53461 tacgacaagc cgacgattta ctatccgctg tcgacgctgc tttcgtcggg aatccgcgag
    53521 atcctcatca tcagcacgcc gcgcgatctg ccgatgatcc gagcgctgct tggcgacggg
    53581 catgaactgg ggatctcgct gtcgtatgcc gagcagccgc ggcccgaagg gattgcccag
    53641 gcatttgtga ttggcgctga cttcatcggc caaagcccgg tgtgcctgat cctgggggac
    53701 aacatctttt ttgggcacaa cttggtcgac gatctgaagc gatcggcggg gctgaccacc
    53761 ggcgcggaag tgtacgccta ccacgttcat gatcccgagc ggtacggggt gattgagttc
    53821 gatgcgacgg gcaaggcgat ttcgatcgaa gaaaagccgc gcgcgccgcg ctcgaactgg
    53881 gcggtgacgg ggctttactt ttacgacgcc agcgtggtgg acattgcgca acgacttacg
    53941 ccgtcggcgc gtggcgagct ggaaatcacc gatgtgaacc gcgcgtatct gaagaaaggc
    54001 gagctgcggg cgcacaagct ggggcgcggg gtcgcgtggc ttgacagcgg gacgtatgac
    54061 tcgcttttgc aagcttcgca gtttgtgcag accatcgagc agcgccaagc gctcaagatt
    54121 gcgtgcctgg aagaaatcgc ctatctccaa ggtttcatcg acgcggcgca gcttgaaaag
    54181 ctcgccgaca gctacagcaa aagctcttac ggtgcgtatc tgcggcacgt tctgagcgaa
    54241 aaccgcggcc aggccagcgc cagctaattt gttcgcgccg ttgtcgttga caacgatggg
    54301 tgcggcgacg caatcttgcg ccacatcatg tcccctgccg cggcctccca ggaggaccaa
    54361 acactgtcta tgccgagcga taccaccact cgagtcggtg caaacaccca gttcgattac
    54421 gaagggattc ccgccggcta ctacgaccgg gtgatgcgcg aaggcaaccc gattcggcgg
    54481 ctgtggcacg tctcgaagtt cgagcgcgta ctcgactatc tgccgcagcc gtcagatccg
    54541 cacgcagagt cgtcgatact cgacatcggc tgctttgccg ggacatttct gtcgcttgtg
    54601 ccccaggatc gtttttcgcg tcagcttggc gtcgacattt tgccggcgca gattgactac
    54661 gcgaaccgca cgtatgggac gccatttcga tcgtttcggc acgttccgag catcacccac
    54721 ctgcgcgaca tcgaaggcca cttcgactgt gtgacgctga ttgaagtgat tgagcacctg
    54781 accgctgccg agattcacgc gctgttttcc gagctggcgc ggctgattcg gcccggcggc
    54841 aagctggtgc tgacgacgcc aaactaccta agctgctggc cgctgctcga agtcatcttg
    54901 aaccggatgt ccgaagtcag ctacgaggag cagcacatca cgcgctttac ctacttcaac
    54961 atggagcgac ggctttgcga gctgtatcca tcgtttgcgg cagagtttgc gctggatctg
    55021 aagacgacga cgcaccttgt gaccccattt ctcgccgggc tttcgtatga tctcgcgcgc
    55081 aacttgtcgc ggatggtgcc gcatacgagc tggcggatgc cgtttggcaa cttggtgctg
    55141 atggtcctga cccgtcgcta agccagcttg gtttgtgcgt ctgggttatt cgtcgtcgct
    55201 tttgggcgaa agcaggtact ggtagacctc taagacgtcg aacagcgacg gcacggcggg
    55261 gggccgttca tactgggtgt tcgaccagcg cagcgaatgc ttgccgtctt ggatttcgat
    55321 gtagaagtcg atgcctccgt cgtcgcttgg atgcactcgg cgcggcagcc gatggaacat
    55381 gtgcttgcga tagtcgcgga tgacatctag cgcgtcgcgg aggtcgtttt tctgctctgc
    55441 actgagccga aattcgcctt ggtgccgcgt cgtcttcacc gccacggtgt cgtcttgacg
    55501 cacgaccagc accgcgcgat acggcttttt ccccgacggt gtggtgattt ggcttacccg
    55561 gaccatgcga atgcgttcgc cgggacagac atggaccacg gcttcaggtg ggagcgtgtc
    55621 ttgtagctgg ttccagctga tgcaagccag aggaagcagg gccagcggca gcagcatttc
    55681 ttgatcatac agcggttttt gcaccgccac cggcttcgcc gtctgctcga gtggctggtc
    55741 atcaaaattg tgaggctctg tgtacaaaaa aatacatcaa atgaggtacg atatgtttag
    55801 cggagcctcc aatcggtggc atccatctca gatgtagaaa gcgctgctca gcaatgggta
    55861 gccaaagtac caaaaccact cggcaaggat tagacgatgg caagtaagtg ggttggatcg
    55921 cgaatcttct tgggggctgt ttgtagtgtg ctgggcgcct gcgggatgcc gagcgcagaa
    55981 agtccgacgg agccgcttgt gcaaccgcag aagccgatcg ccgtttcggt tcgcccgctt
    56041 cacaacaagc agggggcacc gtctttgtca tcggtcgcga cggccacgaa agtggtttcc
    56101 gattcgcccg agcaagtggc ttgggcgacg ctgcgcggta tcaaggacgg tcacaagctg
    56161 tccggagaag cggtcgagag tgccaagctt cgcgaggtgc acgacgctgg caacgggccg
    56221 ctgattgcgc ggtttgagca agaggtagac ggcctgccgg tgtttttgca gagcatgaac
    56281 atcgccatgg atcgcaacat gcagccgacg atggccagtg gtcggctggc gaccaagctg
    56341 cgcaaggttg ccgagcgctt ccagctcgat gagtcggtcg cggtggagtc ggcgctgcgt
    56401 gcgatgtcgg gcgagcgcgg ctcggcggta tcgatggggg cggcgcaggc ggcacctggt
    56461 ggctatcaga atcgccagtt tgtcgcacag ctgtccggat cgggggccca ggcgtcggtc
    56521 agctcgcgca gtcggaaaat ctacttcccg accgacaagg ggcttgtgcc ttcgtactac
    56581 gtcgagctgg atgtcgcgcc gctcgattcg accagcagca cgatgcgggc gtttgtcgtt
    56641 tcatctatcg acggcaaggt cctgtttgaa aagagcctga cggaagacgt cgcgtacagc
    56701 tatcgcgtat attccgacgg tgcaccgggt tacgtgccgt gggatggcgc gcagggcaac
    56761 gactacaccc cgtatcccaa ggctgcgccg gacggcagtg ctccggcgct cggggtaagt
    56821 tcgctcgtca cgctcgaaaa tgtgccgttc cgccgcaacg atccgtggct gccaccgggc
    56881 gctacggagc tgtcgggcaa caatggctgg gcctacgtgg atcaaatcgc ccccgacggt
    56941 tattccgcag gggacttgct ggtcacaccg acaagcgctg gatcgtttga ccgcacgttt
    57001 gacctgagca cgacggcgcc gtccgccaac gacaccgcca agcgcaccgt cgcgaccaac
    57061 ttcttttata tcgtcaacta cctgcacgac gtgacctatg acgccggctg gaccgaagcg
    57121 gcggccaatc cgcagaagtc gaactttggc cgtggcggcg tcgaaggcga catgatccgg
    57181 cttgaagcgc aggacaacag cggtcgcaac aacgccaatg ccagcacgcc tgccgacggt
    57241 ggtatgccgc gcatccagat gtatctgtgg gatgcttcct cgacatcgct gacggtgact
    57301 gcgccggctg gcgtcgcggg tacgtatcag agcggcggcg cttcgtttgg ggccaagagc
    57361 tacaacatca ccaattcggt gattcctgcg acggatgcgc ccggccctgc tggttccacg
    57421 acggatggat gtgatgccat caccaatccg gcagcgattg ccggcaagat cgcacttgtc
    57481 gaccgtggca actgcaactt cagcgtcaag gcggtcaatg tccaaaacgc tggcgcggtg
    57541 ggtctcctta tcgcgaacac catggccgga gcgacggcgt ttggccctgg cgggactgac
    57601 ccgacggtaa ccattggcgt ccaaggcata agctacgccg acggcgtgaa gctgcggacg
    57661 gcggcgatgt ccggcacggt cacggccaag atgacggcaa gctacaacga gcgcgacagc
    57721 tcgctcgatg gctcgatcgt ctcgcacgag tggggccaca tcctgtcgaa ccgcctgatt
    57781 ggcaacggat cgggacttgg caacaaccag ggccgtagca tgggcgaagg ctggtccgac
    57841 tttaacgcga tgctgatgac cgttcgtccc gaagacgcgg cggtcgcgag caacaacaac
    57901 tggtctggca cgtattcgac gggcgcgcca tcggtgtttt cgccggggaa caatgctttc
    57961 tatgaaggca ttcgtcgcta tccgtactcg acggaaaaga ccaagaaccc gctgacgttt
    58021 aagcacattc aggaaggcgt ggcgctgccg gccaccccgg ttccaacctt tggtgcaagc
    58081 ggcgtcggca attccgaagt tcacagctcg ggcgaagttt gggcaaatgc gctgtgggaa
    58141 tgctacgcag cgctgcttcg cgacaacacg cggctttcgt tttcgcaggc ccaagagcgg
    58201 atgcgccgct accttgttgc gtcgctgaag ctgacgccga acaacccgac gtttaccgaa
    58261 gcccgcgatg cgatgttgtt cgcagcgttt tcgagcgacc gcaaggacta cgatctgttc
    58321 gtcgatgcct ttgccaagcg tggcatgggt tcgggcgcag tttcgcctga ctctgcatcg
    58381 accgaccacg caggggttgt cgaaagcttc gccaccggcg ccgcgctgta cattgacaaa
    58441 gactcgcttg cggtcgacca gtcgcttatg gcgtgcgata ccgacggcgt gctcgacaac
    58501 ggtgagtcgg gctacctgag cttccgcatc tacaacaccg gcagcaaaga cgtgcaaaac
    58561 ggcaccgtca cgatcacgac gccgacgggt agctcggtga ctgtcaatgg ccagccgacg
    58621 gccacgctga gcctgccgcc gattccgccg ttttcgtcga atctggtcca ggtcaaagtc
    58681 gcgatggccg gggcgcagag catcgtcccg gtgcagtttg acatcgaggc caaagccgac
    58741 aatctggtgg gtccgtcgcc gatcaccgcg tcgtatatgg acatcggcaa ctacgacatg
    58801 aaggcggcct catcgacggt ggaaaagttc gccggtcgtc cgacgctgtg gaaagtaagc
    58861 aacgatgcaa accttgacac gtcggaagca tggacgccgg tgataaacgc aaacgctggc
    58921 tacatgcacg gaccggatac cggcgtgccg gcggatcatt atttggtgtc gccaacgata
    58981 gatgtcggca ctggcaactt tacgctgtcg ctcaagcatc gctacagctt cgagaccgac
    59041 acgaccgtca cgccgcccga ttactacgac ggcgctgtcg tcgagtacag ccttgatggc
    59101 aagacctgga cggacgcaaa ggatgtgccg ggtgtcatcg tgatgggcgg ttacaccgga
    59161 acgcttgcga cgtataccgg aaacctcaat ccgctcacca atcgcagtgc ttttgcgggg
    59221 gcgaccgcag ggtatgccac tggcgcgtgg gtcacgagca gcttcaattt cggaacggcg
    59281 ctggctggca agaagttgac tctgcgcttc cgcatcggca gtgacaacgc ttcgggtgac
    59341 gaaggctggg acatcgacga agtggcggtg acgggagcca atgcgccgcc gttccaaagc
    59401 caagtcctgc agagcgctgc ggcggtctgc aaggccaagc cggtgttttc catcacgcaa
    59461 gctgacctaa agagcatccc gaccggcttc caagtccggc tcgacccgac gatcgccaac
    59521 gcgccgggcg caatcagttg gacctggacg cagctcgatg gcaccccggt gcagctcgga
    59581 aactcggata cgccgaatgt gacgtttacc gcgccagatt cggggacggt gattcgcctc
    59641 aagtgggcgg cggtttcggc gggcgtgacg cttgaaggca cggtcgccat cgaggtcgtc
    59701 aaagccggtg attcttcatc gggcaccgct gcgacgggct gccagatggg tggtcaagct
    59761 gcgccttcgc tgtggtctgg actggtcgcg tcggcgcttc ttctgctcgg tcgccgtcgt
    59821 cgcaagagct aatcccgccc cttctctgcg gcggcctcgt tattcgacga cgatggcgcc
    59881 gcggagcatg ttcattccgc acgtaaacgt cacctcgccc ttctcttgtg gaagatgaag
    59941 ggcgatttcc gtcggttttt cctccgtcag cgtctttttg atgttctgcg aaggcaacac
    60001 cacttcacgc ccgcactcgc cagcgctttt gtcgtagcgg aagctcagcg tcagatcctc
    60061 accggggcgt cccatgatgc gcggcggcgt aaatccttgg ttgctcaccg tcacttcgat
    60121 gcggcgcggt ccaccgcttg cgggtggtgg tggctctgat ttgcggcatc caccgccgaa
    60181 aatgcctgtc gttagcaacg tcacaagcag cgctgcctgt tgcacgcttc gcacaagggt
    60241 cttcataacc acccacttta gtcagaagcc ggccaatccc aaattttcca cccagacggg
    60301 ccggacacag gctcgcagtt gcacttgcaa aatcgcgcgg cggcggggta acactgcctg
    60361 cttcatcgct tggacgctcg ctcgtcggcg atggcttagc gaaaagctta gaaggagaac
    60421 ccaacgtgac cttgcaaacc ctgcccgaca tcctgaccga agccagccgc cgcaagtccg
    60481 tgatcgctga ctgtgtgctg ctcatcgagg aagaagtggc caaaaagggc ggccttacgg
    60541 gaatcgccat caagggggcg ttcgccatcg tcaaagctgt caagccgggc ttcttggccg
    60601 aagcggtgga tcacatgctc gacgacttcg cggcgcggct cgatccgttt tatcagtcac
    60661 aaaagcaagc cgcgggacgt tccctgccga gtcactttgc cgaaaacagc ggaccgattg
    60721 ccgaagcgct gcttggcatc accgatcagc gggcgcagcg ggcgcaaaac cagacgctca
    60781 agaagacgta tgaaaagctg cgtccgacgg caaagaaaca cgtagaagaa gcagtccccg
    60841 gcattggccg tctggtcgaa aaatacgctg ccaaggtggc cgcctgagtc ttctcgcaaa
    60901 gcttcgccat ctggcgagtc gcctggcctt tacgcttggg gctcgcttct atgcatggca
    60961 gacctggcac gaggtctggc gtcagcactg cggcagtctg gcagcccact ttcctccgcc
    61021 acttccatcg ggacggctct ctgtgctcga cctggggatt ggtccggggg tttcgggcat
    61081 tggcatcttg cagcggcgtc cggatttgtg gcttgtcggg ctcgactttt cggcggacat
    61141 gctcgacctg gcgcggcact acctgcggat ttcaaaccat ggatttccgc tggtccatgg
    61201 cgatgtcacg cacctgccgt ttgctgacga gtgcttcgat gtcatcaccc atcacagctt
    61261 cctgtacctg ttgggcaacc gcgacgctgg gctaaaagaa atggcccgcg tgctaaagcc
    61321 cggcggctgc tacgtgatgc tggaaccgaa tcggcgcggc agcgtgctag cggtacccaa
    61381 gttaggtggg ccgttccgct ttcggctgtc gatgctgttg tggcggattg tttcgacggg
    61441 ctttggccag tttttgcgcg aagaacttgc cgcgctgctc gaaacgcacg gctttgtctg
    61501 tgaacgtttc gaagacacct tgggcggcat gggcttcatc gtcgtggcga aaaagccgct
    61561 gtcttctgcg ccgccttcct ctttccccaa accaaccgat ttgcaaggag cctaagatgc
    61621 agtttcctga ataccgaccg cgcaggatgc gccgaagcga tggcctgcgc cgcatgattc
    61681 gcgaaacccg tctgtcggtc gacaacctcg tgtatccgat ctttgtctgt cctggtcggg
    61741 atacccgtcg tccgatttcg tccatgccgg gacaagcgca ggtgtcggtc gaccaggccg
    61801 tcatcgaagc aaaagagtgc ttcgatctcg gaattccgtc cgtgatcctg tttggcgttc
    61861 ccgacgatgc caaaaaagat cgcgtcggca gtgatgcctg gaacggtgac ggactggtcc
    61921 agcaggcgtg tcgtgcgata aagcgtgctt gccccgaact ggtcgtcatc cttgattcgt
    61981 gctttgacga gtacacgacc cacgggcact gcggcgtcgt gatgcctgaa gcgggctggc
    62041 cggatgtcga caatgacgcg acgctcgaaa acctcgggcg ggtggtggtg tctcaggcca
    62101 aggccggcgc tgacatggtg gcgccgtcgg gcatgatgga tggctttgtg actgccagtc
    62161 gtgaagcgct cgacgaagcg ggctactcac acgttggcat catggcctat tccgccaagt
    62221 acgcgagctg cttttatggc ccgttccgtc aggccgccgg cactgccagt ggctttgggc
    62281 accgcaagag ctaccagatg gacccggcaa acagcaacga agcgattcgc gaagcgggtc
    62341 ttgatgaagg cgaaggcgcc gacgtgctga tggtcaagcc ggcgcttgcg tacctggatg
    62401 taattgcaaa gctcaaagcg gaatacgacc tgccgattgc cgcctacaac gtctcgggtg
    62461 aatacgcgat gctgaaggct gccgtggaaa agggtctgct cggcgaggag caagccgtcc
    62521 ttgaagtgct gacttcgata aagcgtgccg gcgctgacat catcttgtcc tatcacgcca
    62581 aagacgccgc ccgctggctc gccaaaaagt agcgcttgcg cttaaacccg atccactgcg
    62641 ccggcaaaac cgctacgatc acaaaccaca cgatgcttgg tgactctcgg gaggacgtat
    62701 gaagactcgg acgtatcgtt ggctgcgcgg gcttacggcg gcagcgcttt tgtggggtgg
    62761 ttccggctgt ctgaagctga cgctttacaa tgcgtcggtg atgaagccaa gccaggtcgc
    62821 cgtctacttt tcggtgacgg actcgggcgg caatccggtc gccaatttga ccgccgacaa
    62881 gttccgcatc tacgaagaca acggcctggt gtcggtctac gaatcaaagc agacgatcct
    62941 aaaccccgaa gtcgctgcgg ttcactacac cctgctgctc gtcgaccttt cgggttctat
    63001 cacccagtcc ggcgcgatgg aacagctcaa gccggcggtg cagagctttg ccgatcgtgt
    63061 cggtagcctg caaaaagttg ccatctacgg cttcgacggc agcaaggaaa tcttcccgat
    63121 catcggcttt accgatggtg aaggcggtgt ccgtgccgcg gcagagcgtc tggcttcgtt
    63181 caagggccgc gatccatcga ccaacctaaa cggcgccgtc atcgaagggc tcaaggtact
    63241 cgacaagagc ctcgaacatg cctcgacgcc gctgaagttt ggatcgcttg tggtgtttac
    63301 cgatggcacc gaccgcgcaa aacgtgtgct gcggcccgat ctggacaagt cgctcgacga
    63361 agcacagcag aaagacaccg cgctgtacgt catcggcgtt ggcaaagaaa tcgacagtgg
    63421 cgagctacgg aacatcggtc gcaccgcatc gtttctgagc accgatcctg ccgcgcttgc
    63481 caaagcgttt gactcgatcg cgagccgcgt cgaagcctac accaagagct tttaccttct
    63541 gtcgtactgc tcaccggccc gcgcgcaaga acacgacctg aaagtcgaat cgggcggcgg
    63601 cctttccaaa ggcagcttga gctaccactt cgacgccgcc ggcttcggcc ccaactgcga
    63661 ctcgaaccgc atgccgaagt ttgacatcca ccgccccatc ctcaagtcca aaaagtaaca
    63721 acagaccggg cagcggctgc tcttcctttg cgtcgttctc tgcgacgcgc aagcccgctg
    63781 tgccgaatct acgttgttac tttttcttcc catttctgac ataagctggc tcatccgttt
    63841 gattttttac cgatcggaac ttctgcgccg cgttcgtttt gtcgcaccga tcgaactgag
    63901 tccgctttcc caaccaaaac ccgaggagaa atacgatgaa gatgacactt ggcacatcgc
    63961 ttggacttgt ggcggtagcc ttcgcagcgg cttgtggtga gcccgagccg agcgacagct
    64021 tggacccggt gctaaaaggt gagatcgaag acgcgctggc ccgctttgat gcggacccgc
    64081 gttcgtttat gaatgcgctg ccgcaaaaag tgggcgcacc aagtccggcg ttctctccat
    64141 cggtggtaaa gagcctggac tacatctcgg cgaagaatgc gcggcgtcaa gacggacccg
    64201 ccgatgcacc gggctccaat gatcaggcca aaaacctggt cgacaacttg caactgacaa
    64261 accttgccga catgcacagc atccgttcgg catcgcttcc cgagtcggcc tggtcgagcg
    64321 actactgggc gatttacgcc ggtgtgatcg cgaagcgtta caccgacccg aactttccat
    64381 cgagcgaaga ctggaaagtc ctgaccgact acgtgagaag caagcagccg tcgaatcccg
    64441 acgatctgtc gcccgcggaa aagtacgact tgctggtggg tgacacgagc aagacgatga
    64501 ccaagcacaa cctggacgaa ggccagtact actactcaag ctatggcaag gtcgaaacct
    64561 ggatgggcat ttgccatggc tgggcaccgg cgtcgtttat gatccagcgc ccgaccaagg
    64621 cgatcgaagt cctggcggct gacggcaaga ccaagatcaa gttctatccg tccgatgtca
    64681 aagcactggc gagccttctg tgggccaacg ccagccccac gaccaagttc atcggcggtc
    64741 gctgcaatgt caaaaacccg gcgaccgatg caaatggccg catcaaggat caggcctgcc
    64801 gcgacaccaa cgccggcacc tggcacttgg cggtcgtaaa ccagattggc aagtcgcagc
    64861 gctcgatggt catcgacgcc acctacgact acgaagtctg gaaccagccg atgtatagct
    64921 attcctactc gtactttaat ccgcagacca acaaggcgta ctcgacgcta agctacgcca
    64981 aggtcgcggt ggccgattac accaccgaca agttcaagtc ctatcgcgcg cctggcacga
    65041 cgcacattgt cggtgtgtcg atggatgttc gctggaccgt cgaaacgtat ccgtcgcact
    65101 cgacgactga cagcgcgtca cgcgatgcga tctcgggcgc ccgctacctg tacacgctag
    65161 agttggattc gaccggcaag atcatcggcg gtgaatggca ccagaacgcg caccccgact
    65221 tcttgtggct ggcgcctccg ggcacccgcg cggtcgccac gggcgaaccg accggaacgc
    65281 agcctgtgtg ggatggtaag accgcgattc cttcgacgtg gcagacggcg gctcgcacgg
    65341 ccagctcaaa tggcatgccg ctcgcgaacg tcgtggaatc cctcgtccgt ctgtcccgcc
    65401 tgtagtcttt tccagcgtct tccccaccca atcagcccat tggcttccca gacaccggca
    65461 acacggcgct gggacagcca actggctgaa aaagagccga ctgcgcggtc acacggcagc
    65521 caactggctg attttgcgcc tcctccgcgg cttcaaacca aggttcacct tgaaaaaagg
    65581 cccaaagggg ggcgtttgtt taaggttcac cttgaaaaag agcccgaggg gaggcgtttg
    65641 ttcaaggttc accttgaaaa aaggcccaaa ggggggcgtt tgttcaaggt tcaccttgaa
    65701 atggagcccg atacgcggtg cccagccaag gttcaccgct tcccacgcta tcgcgggcgc
    65761 tcgtctaccg taggagtgct tgctggcgga agtgagggag gcgtgttttt tttggtcgag
    65821 cgccgtttgg tcggggcgct cgattgggtt ggttggcgcg ctgccagtct ccatgacgac
    65881 acgactgtgc gcaccatcgc ggcaaactct tggtctgcgc agctgagacg ctcgaactca
    65941 cagagaatca tctctcttat gtacggtgcg ggggttcctt cgagcagctt ttgaaggctc
    66001 aaaaggcggt catagtgatc acgccgtcgg ttgctggtgg tgccactcag cagcagtcga
    66061 accgtcgttt tgccacgccg agaaagtccg gtggccttat acccgtggaa cgtgatgtgc
    66121 tgagccgggt cgtctcggta tgggttgata agcagtggtt tttcggcatc gagccgatca
    66181 tgatgactcc gcgcccgttc tttggggttt tccagcggaa agagaattcc cttgccggtc
    66241 ggcttgccac gcaaatcccg gcgatgctgg ttgcactgca cacagacgag caaaaggttc
    66301 gtccagtcat aagccaacca gtaatagccg ggccgttcga tgcgtgcccc gcggcgttgc
    66361 tgcacagcgg acttggggcg atagtgctcg acctcaaatg gcccttgctt ggtttcacaa
    66421 taggcgcatt tgtcccgctg cgcggttgcg agcgcttgcc gcacgtcagc cgctgcatag
    66481 atgtcctgac gaaagccgaa atcctggtgg ccttggcgct gcgggccggc ggaatcgtag
    66541 gtagagcaga gtttctcggt cagatgccga ccttttttcc caagtgactt cggtgtgttg
    66601 gggcgagtca gtttcagcat cgggtgtccc gctggttgcg gcgtggtttt tgtgccgact
    66661 gggccgcttc ccgaaggatc gcaaggtagc gctgttcagc ggggcttcca atcgcgccac
    66721 gcgcagcaag ccgcttttca atggcatcca gatccgcttg ttcttgcgct gtccgtcgtc
    66781 gtttctggag cagtgaggcc tgccgtccat agtcgtcatc aaactctggt gcccaggcgc
    66841 ttgggacccc tgtaaacaga tcgcttgtca gaatttggtc aatgcgccag ttctgtaccg
    66901 acgtgcgttc gttgtcgatg acgacgtgtc gcgccttttc tggccggcgg agtacgacca
    66961 aattggcatc ggcacccgct gcttgtacga cgagaggact gtgggccgtg accacaaact
    67021 gaacgttcgg aaagtgtgtt tctaactgcc gaaacagttt ccgttgccag cgtgggtgaa
    67081 ggtgcagatc gatttcgtcc accagcacca cggcgttttc ttccagcggt cgcttgctat
    67141 cgggatatcg cacaaacatt cgcgccgcga gatcaagaat ccaggccgtg gtcgtctgat
    67201 agcccaggct gagctggcgg agcggcacga atccgtaggg cgtcttggct tggacttcca
    67261 tgaaacccgt tcgtgagcca ggccggatgg ggacgcagcg aatgtcctca atgtcatcat
    67321 cagggaagag tccgcgcaga acctgcaccg cctccctgta ctgagaagcg gccttggttt
    67381 tcgtcccccc ggctttcgtc gccagcatga tcgaatgttg atcacggagc cattcttcgg
    67441 cattgagcag tgcggcgtcc gggttaaaca gcgtgtctat cgctgcatcg tatcccacgc
    67501 gatcgagcac agggtcgggc cacagggggg tttttgaaag cggacctgtg ccaagtcggc
    67561 gcgctgcgcc ataggcatgc agcgccagac gccaattggg actgcttagc gaacctgccc
    67621 ggaatgaatc ttcatcggct tcgattcccg cagtgtcgga cgtgaaatcg gctgatttct
    67681 gcgagagaag cgaaccaatc gcgtaagttc cagaaatcga cacggtcttg gcggacttgt
    67741 tggcgactga ttggcggtcc cagtgccagt gcaaatccca aaggccgtct tttttgacgt
    67801 tttctgatgg gtagggctgc atcgtcgcca gcgcttggag aagcgttgtc ttccctgtcc
    67861 cgttgtcccc taagatgatg gtccactgtc gaggtctgcc gcttccatcc gacaggtcca
    67921 aggtctgctt ggggccaaag cagcgcagat tttcaatcgt cacgctgagc aggtaagcag
    67981 gaacaggctt gaaaccgagc gtgcgcgtag gcatgagggt acgagtgcca cagacgctgc
    68041 atagaggcaa ggaactgtaa atccgccagg ttagccacgg tttcaagacg aaccttgatg
    68101 tggatggcgc ttgctggggg gcttgcaaag gcag
//
LOCUS       DAORGN010000003        65771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_124_length_65771_cov_6.754596, whole genome shotgun sequence.
ACCESSION   DAORGN010000003 DAORGN010000000
VERSION     DAORGN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 65771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65771
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_65771_cov_6.754596"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKL17_00590"
     CDS             complement(<1..876)
                     /locus_tag="PKL17_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK43247.1"
                     /translation="MAHLQPAKKSIFSVLLAVCLSACTATPEPEAIYVPPPTSTVVEQ
                     GQVAIRREIFLVSAPAPAPNPAVSSGGQTPAQFNFVRVVRYRVDTGDAPPKPARAILL
                     LMPGFLGGAGSFEPLARAMVRRSAGEHAIEAWAIDRRSNLLEDQHGLDVAEVKQDPEI
                     ARRYYFTGEEVEGRTYQGVRPQDELAYVSEWGLKTQIDDLRAVVSLIPQAERRARLFL
                     LGHSLGASIVEEYAAWDFAGTAGYSELAGLVLVDGTAREEGKASVPYREDEYVNGLTG
                     GSFGKLSLTKVRQSDR"
     gene            1063..2097
                     /locus_tag="PKL17_00595"
     CDS             1063..2097
                     /locus_tag="PKL17_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNK43248.1"
                     /translation="MLFDWVYGLFSNDLAIDLGTATTLTFVKGRGIVSNEPSVVAVQR
                     SSAGSKRVLAVGKEAKEMLGRTPGNIVAVRPMKDGVIADFEVTEAMLRYFIMRAHNRR
                     TLVKPRIIICVPSGITEVEKRAVRDSALAAGAREVYLIEEPMAAAIGAGLPITEPSGN
                     MIVDIGGGTTEVAVISLAGIVVSKSIRVGGDKLDEAIVQHIKRKYNLLIGERTAEDIK
                     KQIGSAYPLDEVLTMDVKGRDLVAGVPKTLNINSDEIRDALAEPVNAIVEAVRVVLER
                     TPPELSADIVDKGIVLSGGGSQLKGLDLLLRDETGLPVVVSETPQLAVVLGSGKALDE
                     LLLLREVTVQ"
     gene            2107..3000
                     /gene="mreC"
                     /locus_tag="PKL17_00600"
     CDS             2107..3000
                     /gene="mreC"
                     /locus_tag="PKL17_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNK43249.1"
                     /translation="MSLFRRAKGLLIGAALLLALSLVLRMSVRSPEKQTLLDRAVVRL
                     LAPLQGLSAGLLGRISSLWTGYVALVGVQQENLALKKQNAELRSQLTSVSALAMRAQR
                     LERLLDLRAQVLADTAVAQVISVETSRQFRVVRVRIDRGGVEVKPGMPVIAAEGVVGR
                     IARSVGPYADVQLLTDARSAIDVVLPRSESRGVMKGTGSDSRYVSRIEYVLKKESIEV
                     GADVLSSGLGGSFPRDLPVGKVTAVRKSDASLYQEIEVEPSVSFAHLREVLIVLSPPP
                     PPDPDAGKRGFEAARGPGVPR"
     gene            2997..3551
                     /locus_tag="PKL17_00605"
     CDS             2997..3551
                     /locus_tag="PKL17_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43250.1"
                     /translation="MNGSRAIGLWLLAVLGSVLSTGLSPLTSFALPDFALLVALYAGL
                     TSRHSGGVVAAQRDASALGMLSLGAAMGYASDLVEGTPHGLRALGMALLLLILHRVAG
                     HMLVGGRAAQGAVAALALLLFRLLLTLIMLFADGDRDLLVSGLRSLPMQLVLTAGVAP
                     LALSVLSRIDARLRSKATGPGLWI"
     gene            3548..5530
                     /gene="mrdA"
                     /locus_tag="PKL17_00610"
     CDS             3548..5530
                     /gene="mrdA"
                     /locus_tag="PKL17_00610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNK43251.1"
                     /translation="MKLFRRQKLHFDLRIGIGSSLAPSGVTAGQAQTASLRAPLPVRV
                     AAVLSVLVFVGLAGRLVQLQALRGDDYLQKSTSNFIRERELPSVRGQIRDRRGEVLAD
                     NRPTFAVYATPRYVSEATLLRLGRLLKLTEEQREALSKKLTVRRGAEASKQLLVAEDI
                     TRDQLAALESEKDQLPGIDIEARTRRVYPQKQLAAHLLGYLNMAGPDDLKHGYKHGDY
                     VGRSGIEQKLEQKLRGSPGFERIFVDAHGRRKSEHELGEMAQLAPQEMRREPQPGINA
                     VLTIDLQLQRIVEEALSRHASAAAAVVEADSGRVLALASYPSPDPNLLSGRLSRGEAQ
                     RMESDPFRPLLDKALRETYYPGSTFKIIPALAALEEGLVDPDQKQVCHGRYELGRHVF
                     RCMKTHGPVSLHEALAQSCNVYFYHLAEKVGLERMANWADRFGFGQPTGLGLGEASGF
                     VPTLEYYKQHGGFRGGYALNTALGQGAVRASVLQLAMAYAALGNGGKLYQPYIVERLE
                     TPSGEPVERFSPQLRSQLPILPSSLERLRRALVDVVADNKGTASTAFIDGLDIAGKSG
                     TAQVRKNRRGESSGWDQLNDHAWFVGYAPSRNAKIAVAVLVEHGGLGGHIAAPTATKI
                     VQGYFDRVAPELKPRPVADVSTPKLRTTLPASAGAR"
     gene            5542..6633
                     /gene="rodA"
                     /locus_tag="PKL17_00615"
     CDS             5542..6633
                     /gene="rodA"
                     /locus_tag="PKL17_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNK43252.1"
                     /translation="MLRRTWSLIDWPLTAVVLTLIGLGLLNLYSATRVAPKGMFSSQL
                     LWIAVGTLILIAVAAFDPKWLRRLAWPAYLGVTGLLVAVLLVGKVVNGSRRWFGWGSY
                     GIQPSELAKLSLVLLGAHLFSSDPQGMTHKPWHYAMKWHALWALPALLILKQPDLGTA
                     LLCGLLALSLFFLVPLPAKLKGWVLTVGLSATVLVMRFGLKEYQKKRLLTFLHPELDP
                     TGTGYHARQALFAIGSGKFFGKGYLRGTQNHLQFLPEHWTDFPFAVWAEEWGLFGCLA
                     MLSCFLFLILWTLHIAASCRDRFGSYLVLGCAALLFWHVSINIAMVIGMAPVVGVTLP
                     LISYGGSSVLTVMAALGLIYNVSARRFSY"
     gene            6666..7301
                     /locus_tag="PKL17_00620"
     CDS             6666..7301
                     /locus_tag="PKL17_00620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNK43253.1"
                     /translation="MTPALEEYLTSHSTRRDDVLSALAQETAQLGDVSVMQIAELQGS
                     LLSLLVRATSAVRAIEVGTFTGYSAICVARALPPHGKLLCCDVSDEWTQIARRYFAQA
                     KVEDKIDLQIGPALETLRRLPQEPLFDIGFIDADKVNQQNYYEEVLTRLRSGGLLLID
                     NVLRDGRVTDPTQSDPSLDSVRACNDAVARDPRVEVVMLPIADGLSLIRKR"
     gene            7360..8367
                     /locus_tag="PKL17_00625"
     CDS             7360..8367
                     /locus_tag="PKL17_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNK43254.1"
                     /translation="MKSPMKRSLAIGLLFVAACPETKVSGLGQAPIEASSGIDATDEP
                     ISPLPISLPLSAEKIALGELLFASPLLSADGSLSCLGCHPLDRGGTDGKIYSQGVHGK
                     LAAVNTPTIFNVGFAFRLNWNGAFSSLEAQLSRPLEVLMGSSISDVLTRIRSDKNLSR
                     RFSDIYPQGVTETNFRDAIATYERSLFTPNARFDRYLRGEKNAISDEELQGYKLFKEY
                     GCVSCHQGQNVGGNMFQKFGVMGDYFADRKKAILDGEQGRFEVTKKESDRYVFRVPSL
                     RNVALSAPYFHDGSAPTLEAAISVMARYQLGRSIPQPHVIKLAAFLRTLTGEFRGKPL
                     Q"
     gene            8364..9959
                     /locus_tag="PKL17_00630"
     CDS             8364..9959
                     /locus_tag="PKL17_00630"
                     /inference="COORDINATES: protein motif:HMM:NF039312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK43255.1"
                     /translation="MIEQNKDSQSLPDAISVGFPKAFGSPVSTVILLLIVLCGLVLTA
                     LVVTAKKYTVDARLHEKYSGSLRSLIALDAQLNEAVLKTSSGMSVSYDPLQRLLRQLH
                     SQSVELQNTTWPIPYFPQEGSVSVEKSLTEFLSLLADKEVLIEKFQTETSVVRNSHNY
                     FPVGVRQLVDARQKESKHDPLSSSLHELLQCVSLYTLLPNPEHSVACLHTLASLQQNL
                     HVLSGTDQQEVRLLLLHAQVILDREQRLSEAVRSVLSLKTRDVADHARKRYDDQYQVA
                     LRRGDVYRLSAAGFFALLIVLLASLLIHKLRRSAAALQHERELSEQLLLNVLPKPIAD
                     RLKQTPEVIADSFPTVTVLFADLVGFSQLSAKLPAIDLVRLLNRIFSALDLLAQKHGL
                     EKIKTIGDAYMVVAGLPIARADHAEAMAEFALSAISTLDSLRSELPQDMSFRIGINSG
                     SVVAGVIGLRKFSYDLWGHTVNLASRMEMHGIPGAIHCAESTYELLKNRYEFVPRGPI
                     PIKGIGPQTTYLLQKRKAADPAA"
     gene            complement(9956..10717)
                     /gene="lolA"
                     /locus_tag="PKL17_00635"
     CDS             complement(9956..10717)
                     /gene="lolA"
                     /locus_tag="PKL17_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNK43256.1"
                     /translation="MRRFLPKLSVLPFVLSIAAAPPAPPAPTATPAATAAKNPAATPA
                     TTPRKATDAAIPARPPVAEVVSQLQKSYESIASLRAKFSQTLTGPMGKRQASGTVALK
                     KPGKMRWDYEKPEKKLFVADGTTLWVYEPDDEQAYKQSLSSSQLPAQVSFLFGRGKLQ
                     EEFEISYFDDQPLGEAGDLVLKLLPKVASAQYRHLLFVVQPKTFLVKETVLFDQQGGQ
                     NHLVFSGIEPNPKAGVDDARFSFTPPPDTKIITPR"
     gene            complement(10814..11161)
                     /gene="trxA"
                     /locus_tag="PKL17_00640"
     CDS             complement(10814..11161)
                     /gene="trxA"
                     /locus_tag="PKL17_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNK43257.1"
                     /translation="MSSHHIKILTDANFQAEVLSSDKPTLVDFWATWCAPCRAIAPAV
                     ESLATQFEGKLNVGKMDIDHSPSTPTKYDVRSIPTLLVFHKGKVIGQLVGAAPKPKIE
                     QFVKDALAKTAAH"
     gene            11248..13548
                     /locus_tag="PKL17_00645"
     CDS             11248..13548
                     /locus_tag="PKL17_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNK43258.1"
                     /translation="MSLDRTETAQPLLPLPQLPKLPLLDGQVQVDGIVSSQVFVSPEA
                     DFAVLRLDVAGQIRPVIAVGALAGLRIGETVRIIGRYEQHDRYGQRLRADQALPQTPE
                     SRLGVERYLSTLAGLGPELARRIVAELGPRALIALEEETFRVAQIKGVGKKRAQRALI
                     DARARREEREVMIFLQGLGISAAYAARIRKQWGQTAITKVRENPYALAREVTGIGFHI
                     ADRIAAAMGVEPLSPLRCVAGGRHVLLSACDSGHCYLPTKELLQRAVQLLTQAPPDDH
                     EPNRPLPPPDEKLVDAVEKSIETMRSHGETVHEGDAVYLSSLHQQEVDLTRQVSRLLS
                     TIRARIPTISEHDISLRLAEGQKAALLRVETSPLSIITGGPGTGKTTIISALVRAYLR
                     AGLRVCLTAPTGRAAKRLSDATGQTAKTIHRLLNLIPGERGRVAKDLEIDLLVCDEAS
                     MLDLPLALTLLRAVPGGATVVLVGDVDQLPSVGPGRVLADLIDSTCIPVTRLREIFRQ
                     KEGSGIIDNAQAILRGELPLSAPSDGKLADFYWIEVDDAQKSRDTVVKLVSERIPQRF
                     GFDPLTDVQVLTPMHRGEVGTEELNRALQAQLNPAAKQPQRPQATTGEPTADKPSTPQ
                     KVSFYVGDKVMQVRNNYELDVWNGDIGVIIGRDEEDDLVLVRFEDERQVAYDAEARDD
                     LELAYAVSVHKSQGSEYKAVVVPLHIQHFPLLRRNLVYTAVTRGKKLVVLVGSRRAVR
                     RAVTQTGDLQRYTGLRERLRSMLPSG"
     gene            13545..14810
                     /locus_tag="PKL17_00650"
     CDS             13545..14810
                     /locus_tag="PKL17_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43259.1"
                     /translation="MNHFPNTSSRNWAYVAVLLAAVVSTWACLAAPLDLGFGAKGDAA
                     VDSGVYPDDGQGQGVLGLFTITGCGKLTFPGDEPRCEGLSPLSLQLVLLPIGVSSYRW
                     QVVPANGTSDGGVSDGGLGSLLDETQSRSRSPSFTLTRPGTYYVNLAVAGPGGTSSAS
                     GQIIVGTGDIGAPCRSDDQCQNGLRCLCAADKPGRDGACPGSLASGLCTKSCDGVACP
                     TGSVCLNLSRSQTALPDGGSGDGFRQPICVMPCSVDTDCRADQVCRELPQSKPGAAAS
                     APLQFGKVCFASIPAGVGASCIGPDEQPSQSDCATGICEALGSHDLCTISCGAGCPQS
                     AACATWNGTVAPVPSGPRCLGRCDASHPCADPLLDCLPGGGSGGLGFTLVGEPMITQV
                     CAPRRCSKAADCPGGRCVTIGTASFCLRS"
     gene            complement(14790..15737)
                     /locus_tag="PKL17_00655"
     CDS             complement(14790..15737)
                     /locus_tag="PKL17_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019022878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43260.1"
                     /translation="MAIIGITGSEGLIGTALAQALVDRGDAVRRLDVCLPIGHAGSGD
                     VRIRDSVRRLVAGCDGVVHLAAVSRVLLGERDPTLCWDTNVLGTQEVIDACVAQAHRP
                     WLLFSSSREVYGQVDQLPVTEDTALRPLNIYGRSKAEAEARVFAARGLGLGTAVLRFS
                     NVYGSTHDHADRVVPAFARAALWGGLLRVDGSSHVFDFTHLADTVRGILATIDRLACG
                     VVLPPIQLVTGQGTTLSQLADLAQKTGGGRCRVVEAPARSYDVTQFVGSPQRARELLG
                     FAATTSIAQGMAALIAAFRALSPKEKAGAGLGPLTAQAE"
     gene            15954..16769
                     /locus_tag="PKL17_00660"
     CDS             15954..16769
                     /locus_tag="PKL17_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK43261.1"
                     /translation="MIRSERTQAMMPIAKSKTVVFVHGMFMTPLAWEKWEAHFAAKGY
                     KTLAPAWPLHDLPIEDQRKNHPHPKLGELTLDTVLSHYRQVLKGLDEKPILVGHSMGG
                     LIVQILLAEGLGAAGIAIDSAPPKGVISLKYSFLKSNWPVISPSAKVSEPFFPTQQNF
                     DYSFSNCIPAAEQKSAYERFAVPESRRVGKAPTEDVAKINFQTARPPLLLIAGSLDHI
                     IPASLNRSNYDKYDRSPSITDFREFEGRCHFIIGQTGWEEVADYVLDWIAKNQ"
     gene            16844..18721
                     /locus_tag="PKL17_00665"
     CDS             16844..18721
                     /locus_tag="PKL17_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNK43262.1"
                     /translation="MTAAASALPLLTKEECLAQHPFPKEFAAAKRLEWFWHYDVDLSA
                     KELWTLVADSSRMNRALQVSEMKFEDRNGQRIGSSRPGGFFHEWQEVPWNWVAERFIE
                     SVRIYRSGFSKVVYAVFMLQPLSTEKTRLYVYFGAVPKNPLTSLALRLGFPSVGREYA
                     KLLPRLADEVRERRPLSLVQPGPGLSEAAQQRLATIRQDLIAKGLDVRAVNQLLDWIA
                     NGDEQELFRIQVRERARAFQVDEDALLRVCLHATRLGLLELSWDVMCPHCRGVSKQNG
                     SLGDISTQGACEVCLIDFGTDSSDAVEITFHVHPSIREVPLRTFCSAEPATKEHIRVQ
                     HSVPPDGKQILLPPLPKGRYRLRWQGHKRYGYLDVGVPSGAREIDWRTSSQPCELSVD
                     SDFALQLHNDSSETALFVLETAQWSDLALRPGRIFSLQEFRDLFREEYLSADVQLAIG
                     QQTILFTDMVGSTELYAKQGDAVAFREVRRHFADVFRIIAEHRGAVVKTIGDAVMGAF
                     TSPLDAVLSAKAIHDCFHPGRSDSLTKLRISVNVGPCIAVKLNTDIDYFGHTVNVAAK
                     LQSLAGAWQVALSDEVYFAPGVADWLAKEPAKLEAVRYESPSLLSPISGKRWTVYEGG
                     P"
     gene            complement(18769..19230)
                     /locus_tag="PKL17_00670"
     CDS             complement(18769..19230)
                     /locus_tag="PKL17_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNK43263.1"
                     /translation="MTAVVRRPVSTQFPSLFSEFDPFRVMEGLLRFGPPQEAIQRSLE
                     FAPSFDVKETKDAFLFKADLPGVKLSDLEITHTGNRLTVGGKREAEERKEGDNYYMVE
                     RSFGSFQRTFTLPESADVDQVKADLNAGVLSIVIPKRTAAQPRKVNVSVGQ"
     gene            complement(19418..20755)
                     /locus_tag="PKL17_00675"
     CDS             complement(19418..20755)
                     /locus_tag="PKL17_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43264.1"
                     /translation="MSLVLATVLCVLSVRAGWATPLAGDAAQEVWLAPPPLVPQLGPR
                     FAVVTVDVSGTLGGTCPAGLLRSLVRRQPLLGDVRVRFLPLRSGGERGAELWTAACGE
                     QPAQCFPLLLELCEHPEWLSPGAQTALGVSSTPTVPGELSEELWRAVARHGFDPASLR
                     ESLRTHRTQRQLAAMWASARGNRQMPEVLVNDRRVVGAQLELRVMDEIDAARLRAHDM
                     LKNGTPLTRLYEQLQTTPDDDRRRLDVIWGSGPFDPQRDIPNRPLRVHLDGVPCQGPQ
                     IAPTTLVLFFHHESFPTGVQTRAVLDAVARFRDKVRLCVLHAPLLPAARRTSELIAQT
                     AAVDPRLFFRLLEDVADLMTRRFVLRYDDVVQLLRKRGELGKVEAASARGRNQVMSDV
                     AELQRLGLRPGMWLIIDGKVWSNWSTEALAQALLQKTQQGFFSRLKKNLSTPP"
     gene            20909..22228
                     /gene="fabF"
                     /locus_tag="PKL17_00680"
     CDS             20909..22228
                     /gene="fabF"
                     /locus_tag="PKL17_00680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNK43265.1"
                     /translation="MQRRVVVTGIGLCTPLGLGREASWKACIAGQSGIGPITHFDHSL
                     FTTHFGGEVKGLDPTTFIDKREAKHLDLFVIFALYAAEEAMAHAQLKVSPEPADKPGL
                     FVPGAQLPFGARSDGSYPCDRVGVFIGAGLGGVSHIESTHSTLTERGPGRISPYFVPQ
                     IIINMAPGLLSIRKGLKGPNMSQVSACATGAHAIGEAARTISEGAADVMIAGGTEATV
                     SPLGVGGFNAMRALSTRNDAPEKASRPFDVDRDGFVIAEGAGLLVLEERESALRRGVP
                     ILAELVGYGRSADGYHMTSPCVDGEGAQRCMRMALRQAHQHGISPTDIGYINAHGTST
                     RQGDIAETVAIKAVFGDHARKLAVSSTKSMTGHLLGAAGGVEAAFSILALQDGILPPT
                     INLDKPDPECDLDYIPHTARAAQSRAALSNSFGFGGTNASLIFAKHI"
     gene            22241..22657
                     /locus_tag="PKL17_00685"
     CDS             22241..22657
                     /locus_tag="PKL17_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43266.1"
                     /translation="MDNRDFRHECVRRGDVLILRVRGEFTLDDARAYVAIRDQIGAEQ
                     NYLLLLIFASEATGMHPAARRYVAEHATPHEGKNSAIAVVGAGAILRGLLQLLFSAIT
                     LLGRRPVFPSFVSDEATALRFLDEQRQRIYKQLGRK"
     gene            22748..25087
                     /locus_tag="PKL17_00690"
     CDS             22748..25087
                     /locus_tag="PKL17_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43267.1"
                     /translation="MPSQPHESAPASHVPATASSHRTALGIGALALVMIYYGIFVIRN
                     FSGPIGDYNESFLHEYLAWYASKHISLWPIPTLHLHTDQVLYPFGANVALQSWCVERD
                     VLFALLKPLFPNAPLLQLYYLLGVGLSTIGSYLLLLPRFGALRSGIATVLAHAFNFYS
                     MQKYAYHFNIACYHWTTLGIVCDFVLVKTVVADRLIPLRLLLLRCLLLALAFGLELGH
                     VLGYSLTSFLFASAYVVWFVRSEQRQGRIAVQQLFHSLRDQLGRDRLFALLMLSSTLA
                     VAWLYGAITFGIVRDSLSVGKFPGQAAAWWASPLRLLIPYTPWLHPSMQPLFNHVLGD
                     LAEDGIGCGSPGLFLLFLAGLGIYQTKAWQRRSPYFFLFASFCLYVLSGAHLPLLKLL
                     PWFAFCRVYSRSTLVYSTLLALFALEVSFSVVRTSIRRSLLLLGILGIGGLELVTVAQ
                     IKRELPAYSFSAQQKDYLATIKKLPGEALLDFPFCLTGGNGDIAGLCPLWQRLKGVYA
                     LQRFHEKKVIGQYLGRLHPNQTAPFVRQGWDTLNDPDTLRMTIAQRQRRCLTSDEWFF
                     FEQFYLYNDFAGIQLAIDRLPPGCPEQFYKRYGAPIASMELAGAGRLVLIPKPLSQHA
                     LVNKEMGKQVTLTRTEAALFKRKLVAADLQVSSPTDVHEITGSEGQPQSYDFVDGDGI
                     VYGPYVALPKGRYVATFELEVPQPGTGTLAKLDVAAHNTSFAATKVQADSLPSGRQTV
                     ALPFELSERTSGIETRVCAKGTRFILHSITLEQLGTQNN"
     gene            25107..25979
                     /locus_tag="PKL17_00695"
     CDS             25107..25979
                     /locus_tag="PKL17_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43268.1"
                     /translation="MQALHRFGTALSVRLSAAFLFGSLLAVTACSPARVGVEIDDMGV
                     PQDLTVRPPRPDLAPPPVYDFALPDVHVVITADNAYAFGFGFADRVTKLNGRPKTVVA
                     GDIFNCPIGVGPEAYDIPAAEAPESSYLYIVAWADDAVTEGVLGQFERSGTPLYTGDA
                     NWQVCATGQFYDPGPAGGGPTLDVVNQELARCNTAAGSPATSSAGWVSTTGAVTPGAI
                     GNLAVGEDNSSPDGDFPIVCQKDAMGQRGIDSGARWMWYSPDGKSPFRYTGGKNPTRT
                     FLIFRLPSRVVVIG"
     gene            complement(25992..26888)
                     /locus_tag="PKL17_00700"
     CDS             complement(25992..26888)
                     /locus_tag="PKL17_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNK43269.1"
                     /translation="MSDGNLTKQRIEGAMLVLLSGITISTKAILAKLAYRLGADALQV
                     LSLRMFMAVPLLLLTAYFSEKKATARLSKREMALTVLLGITGYYISSMLDFLGLLYIS
                     ASLERLVLFLYPTFVVLLAALLFRTPLRRLHVLSLGVTYAGMGIVFHHEQNLSGPLVM
                     KGSLLVLGCSVGYALYLLGAGRLIPKVGAARFNSYSLLSATVAIFVHWIVAGRPLSGL
                     PLPVYGYGLLLATVATVIPTWLLAKGIGLVGSGPAAILTTIGPVSTILLAHFLLGEQV
                     TMGQLIGSVLVLCGVTMVSLRK"
     gene            complement(26911..27705)
                     /locus_tag="PKL17_00705"
     CDS             complement(26911..27705)
                     /locus_tag="PKL17_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK43270.1"
                     /translation="MARYDRIGRDYQRKRRPDPRFAALIEEALGDCVSVLNVGAGTGS
                     YESPNRKVVALEPSEMMIRQRPLDAAPAIVGEAERLPLIDQSVDAVTAFMTVHHWLDV
                     PRGLRELRRVARQRIVILTYLPHGEVFPDRWITNVYFPGIAAAHRRRMPARSFFEEAL
                     GPVTSIPVLIPHDCTDGFIDAFWGRPEVYLDPSVRNSMSAFQLLNPVELVQGIAQLRA
                     DLKHGRWDEQFGKLRNQESMDAGLRLVVAHLSGDRSDEGSDGDSPP"
     gene            complement(27721..28488)
                     /locus_tag="PKL17_00710"
     CDS             complement(27721..28488)
                     /locus_tag="PKL17_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK43271.1"
                     /translation="MSEITLFRGESHSNILLQNLGTSGLAVQANHHLIVHNGECILLD
                     PGGHAVFQRLFEDTAVMSGGAQIRHLFLSHQDPDIVSGINDWLRATDALAYVSLLWLR
                     FIPHVEIEEQFEHRLRAIPDEGMWLDLNGSELALIPAHFLHSAGNFHLYDPTSKILYT
                     GDVGASLGCEYREVPDFEDHVPFMSGFHKRYMASSLALKTWVKLVRTLDIEVIAPQHG
                     ALFRGREMVTQFLNWCETLRCGVDRLPECYELPPRPL"
     gene            complement(28531..28971)
                     /locus_tag="PKL17_00715"
     CDS             complement(28531..28971)
                     /locus_tag="PKL17_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK43272.1"
                     /translation="MERAPQHDQRAKIFVLDDSQTFLTYVQLLLQAEGYVVHSFDSPL
                     GITREVIRHQPDMVIVDVNMPSITGDKVCQLVRAASYGKGVVILLHSSLPEAELSELA
                     RRCGADGSIPKSRDPKLLTQAVERFLARRSRLFSSGTIRHDRAP"
     gene            29422..29934
                     /locus_tag="PKL17_00720"
     CDS             29422..29934
                     /locus_tag="PKL17_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK43273.1"
                     /translation="MGEASKVSGVTQELLIFELGERLYALPSSQVREVLRAFSAVPIP
                     NAPAIVEGLLNVRGDIIPLLSLRRRLGIAERPLSLTDHFIIAKEGSRLLALRVDRVVN
                     LQRASFQRPFDPGRYASGSEVISGVATIPDGMVLIHDLGRFLSAEQSEQLDRAVRDAA
                     GAVREEKEST"
     gene            29931..30536
                     /locus_tag="PKL17_00725"
     CDS             29931..30536
                     /locus_tag="PKL17_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK43274.1"
                     /translation="MSQEPKSHFQQLISRLAKVAAALDESLAHTEARVREQLDARARL
                     MAYAVDRVRSEDQGMEVLAFLLGSQRCAIATRFVIEMFPAPAITRLPGTDPYILGICN
                     VRGQLLLVFDLALLFGFPSQKTDSTMQILLLGAERPEFGVLCCGDGLTLRLPKSSLSR
                     SAELHEPPFFGCIDAVTSDGLLLFDGKELMRDQRLFVNQTG"
     gene            30545..31801
                     /locus_tag="PKL17_00730"
     CDS             30545..31801
                     /locus_tag="PKL17_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNK43275.1"
                     /translation="MSTFRSVLSSTQKQTSYLLLLIVFGGPAFIIVRLLFPDLAGPSP
                     NPNEPAFVLTYGPPLFAALAAMGTLLQLQRYFNQFHRFTTLGQRMPSALPQADSQGRS
                     IGSETRELIDTLISLHSVSQALRSENEQQRGELQRLSSHYESLHQSLLSRMESLLSAP
                     VEELPDRASARQNAPHAMEGLRQNLDDALTRARSQRDLIDQIVRQTHHSHAETESAHE
                     RLLSLRSKSSALSTAMQELMNRTQTVGQIAASVKLLASEIDHVALNATIEAARAGDVG
                     RGFSIVAAEVRSLAEQSKGAAQKMRQVLEEIQQASKSTLSFAMESEQSIEELADTTRQ
                     TAASLLSLENEATEAQDSVSQLLSSMESQTGSVSVIQAALHGTAQGSEQLQRLIHRNE
                     ELRHILYSAQTEKTITASAVSQGARR"
     gene            31798..33066
                     /locus_tag="PKL17_00735"
     CDS             31798..33066
                     /locus_tag="PKL17_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HNK43276.1"
                     /translation="MSSDSFLSRRRFGTLLLSSTASLLAGCPQRSGSSDDAVKVGVLH
                     ALSGTMAIIAKPVAEMTMFAIEEINAQGGLLGRKLVPLLVDGRSDPAVFAREAERLIV
                     DEHVKVVFGLLNSASRRTTKSVFEKYNHLLFYPMSYEGLEQSPNIIYTGAAPNQQITP
                     AVKWCFDNLGKRFFLVGTDQVHSRAANAMIQDQVSAIDGEVVGEAYVLWGSKDVKSVI
                     EQIETRKPSVIVNSITGDSNLAFFRELRRAGITPTKIPTLSFALTEHELAALNTREMA
                     GDYVAWNYLQAQSNPENIEFVKRVRARFGANSVVDDPMESGYLSVYLWAAAVRAARSF
                     SPDLVLGTLPNQSYNAPEGLVYVDAENNHVWRSARIGKIDTDGSFDLVWESGRALRPV
                     PYPIYRTKADWETLLNDLFIGWKRQWMNRG"
     gene            33076..35178
                     /locus_tag="PKL17_00740"
     CDS             33076..35178
                     /locus_tag="PKL17_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNK43277.1"
                     /translation="MQIPTTRELLRILSKPRLATRLLGWFLILTTGPLALIMYITYER
                     GASAIRREVEQSLEATSKARAVAIENYVVERKRNASGLSRIPDIVATMADFHQAFTRG
                     GLKSPAFDEIDQRMRPFLTSVKESRGYSDLFLISPDGDCLFSVAKAEVWGTNVLSGPY
                     RDSSLARVFERTRSMLDSEVSDFDYYQASNDSAAFIAAPIMRGKTLVGIIELQLNNQE
                     IYRLLADYTGLGETGETVVVSLRNDKIIYMAPTRRDPDAAFRRQIWNRDLPKYRALYE
                     SSQGRTSTSAETVTDYGGKQVLAVWRFLPSFRWGMVTKVDIEEATAAITAMRRDQLVI
                     ALFAVLVVIGAALYVARSISEPIVKLTQSTNLIASGNLDERIDISSEDEVGELAAAFN
                     KMTADLKNTLVSRDALKDQIRHRELLTATIRNVVSQLGTTSADILNAAAKLSHGAREQ
                     ASAVKDAVTAVDDVSQTAAQAASRARKVADSVRRSEEVGKAGRKAVTDTVEVLGIAKE
                     RSDKVAENIVALAEKTQAIGEIIATVSEIAEQTNLLSLNAAIEASRAGEHGRGFTVVA
                     SEVKALAEQSKKATGQVRNILESIQKAISTAVYSTEQGIHSMVTATEAAGQAGETISR
                     LSEIIAEAATVSTQIMNSATQQATGMDHVGSAMGSINQIATQNMSATAQTEHAVRELN
                     GIGLKLTELLKDSGQAGA"
     gene            35182..37434
                     /locus_tag="PKL17_00745"
     CDS             35182..37434
                     /locus_tag="PKL17_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK43278.1"
                     /translation="MDKSVLIQRLLETFLVEFDERLQAMSRGLLALEASATGTPERDK
                     NLQELLRNAHSLKGAARAVEVSLIEKSCHHLEEILAAVRNHPTLLDQHLIQLLFGVLD
                     GLAEAGKLLRKQEPLEGSLLFGVALEMEMFIESTIRPLGKEPAQALAAPPPAQIAKSD
                     PNADAENAVSSDLPTVRIHTEKLDALLTRSGELLTARQHLRLTSEQLSQVADSIRRLE
                     SEWRHHGKGNAKIGSPTSKTATKTGDSLVEETPARLTELVAQIDKLQLALRQDLRQLE
                     HTGSALDQQVSRIRMLPFSHACVGFDRMVRDLARNSGKDIELRIEGGDVELDRSVLDA
                     LKDPLRHLIRNSIDHGIERPEDRKRSKKSPRGTIIASATLRGGQVAIVISDDGQGLVA
                     DSIRKRAQKLGMPIPDTDQKLIDAIFMPGFSTAADSVSMVSGRGIGLDVVRNQIKSLH
                     GEVDVSFVPGKGTQFQLTVPLTLTLLRVLLIRAADQTFVVSNNYVHKLLRLRQNEVIW
                     IGERPMIVIDNQPIHLDSFAHALSLRQPTGITWQGKRPVVVLRSEQRTAAFLVDELVA
                     EQEVTVKSLGSRVKRIRNVAGAVIMPDGQVALTIHVADLLHTEKPDESLGALRHGGAQ
                     DSGERKQRRILVVDDSVTTRSLERSLLESAGFSVELAADGVQAWDRIQSGEIDLVLSD
                     VDMPLLNGYQLTQTIRKSELFRKLPVILITSNDDQRSRERGIEAGASAYMTKGTFDQS
                     VLLSVIEQLI"
     gene            37584..38399
                     /locus_tag="PKL17_00750"
     CDS             37584..38399
                     /locus_tag="PKL17_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNK43279.1"
                     /translation="MGVVIGSVTEEVPGLAIRSFLDPEGLRLSEEDREPRSTEAWIHS
                     IVLHTTKGIPGGSDQRRQQILDGSGPHKNHIAAVAQAWASDHICASAHFLIDQDGTIA
                     CVADLLTETTYHAGQRAVNHHSIGIEMYQGEEAELYAFQLEQTVRLVDFLTRRFGIQR
                     QFHAPYRNQAVLRIKEGGSSVVAVYGHREASDDRGVGDPGDAIFSALLAAGYECFDFD
                     KEEDRAVWKVRQTDLNQKLGLSLTVDGVPGPETVVALRTAGYPHGMWIPRPGD"
     gene            38505..39098
                     /locus_tag="PKL17_00755"
     CDS             38505..39098
                     /locus_tag="PKL17_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43280.1"
                     /translation="MMKQTARIVLSGLWLLSGCGTGEEIEEIDVAPLEEKEITATCPH
                     KAQLKWGLHPDASDRLRCIGIAAGDISQTIGSAPASAGYHAQDGTAGGQPYTAAVDIR
                     IGGRSESQIRALLASLGSYGFAAWYRKPGHDGWPSDEAPHIHAVYAGCAMKAALDEQV
                     GDYRAGRNGLSSHTRYTFWTASQAVRDAVYNLWNKYN"
     gene            complement(39676..40002)
                     /locus_tag="PKL17_00760"
     CDS             complement(39676..40002)
                     /locus_tag="PKL17_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43281.1"
                     /translation="MPLAVQKLVVLPATVVVQQGWPAPPQVPQLPFVQVPVRFGQVEP
                     ADEQTLLTQQPTLPATTLPQPLPTQQGWPGLPHGLQMPWPLPVQTSFPSQARPAQQAW
                     PAPPQD"
     gene            complement(40132..40557)
                     /locus_tag="PKL17_00765"
     CDS             complement(40132..40557)
                     /locus_tag="PKL17_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43282.1"
                     /translation="MLLPQHAPEVEQVFPAQHGWLLPPHATVAAFAQTEPVMLGMFCP
                     EATHWPATQQPPPPQVLPGQQAWPGAPQTGQVPALQVAPVEQTDPLARQLFDPGSQQP
                     PAEHVEPAQQGWPGPPQARHRPLPHAVVESVQTLPAQQG"
     gene            complement(40666..41004)
                     /locus_tag="PKL17_00770"
     CDS             complement(40666..41004)
                     /locus_tag="PKL17_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43283.1"
                     /translation="MQQRWPWPPHATHWLLLQVLNAAVHSTPLPQQAWPILPQVPDWQ
                     PLFVHMPSVPGQVAPLAMQVRLLLSQQPPFAQLLPSQHGWPGPPHAAHLPPLHERPVV
                     VQKRAPEVAF"
     gene            complement(41161..41691)
                     /locus_tag="PKL17_00775"
     CDS             complement(41161..41691)
                     /locus_tag="PKL17_00775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNK43284.1"
                     /translation="MRPAPRSHGSGEFSRSQLAACPESVVIEPGVLIFHPENVSLGEC
                     VYVGHRAILKGYYKNELRVGCGTWIGQDVFFHSAGGIEIGCDVGIGPRVMILTSAHEL
                     PATPDGSTAPILHRPLAFAKVTLEDGCDIGVGAILLPGITIGKNAQVGAGAVVTQSVP
                     PRTIVAGNPAKALRVY"
     gene            complement(41691..44561)
                     /locus_tag="PKL17_00780"
     CDS             complement(41691..44561)
                     /locus_tag="PKL17_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNK43285.1"
                     /translation="MRRRFFFAQRLIWLTSLVVVPTLAERAALAVGEQNARLQGVVLE
                     AGTGVPMPGAKVTVRSDAMIGGSKSATTDDDGRFDFLSIPHGSYDITVEYEGLQPIKR
                     RVKLALGETRTLKIDFAAEMAEGETTKIFEERARIDSEKVSTGRVLTSDQQAKIATGR
                     SYQNIVQQLPGVVGGSNPVMAGGSLRHNRYLVDGLDITDPVSNTFSANFNFDAIGQVD
                     TQLLAIDAQYNSLGGVINLVTKRGSDKFSVDTSFYLNHQKLSSGSRAGTQLYEGRLLD
                     QSDPRPPNAQYQLNLNLSGPIVKQKLWYYLSTELRYTASALVPGTPLNLQHATREFYG
                     AYPRLKLTWAPAQRHRVELSFNADPAWIYNTIQSNAYANEAETIQRQGGAFTSLNYDY
                     FVSDNVIFNLQTGVIYSNISVDPMSADKVSPQHIDGANGITWNNAGEARFTDDKRWRV
                     QFDPNITWVKKGWIGQHTFKAGIQGQFIRHYLYTGTPANSVYVDDTSADGSVLARDPT
                     STDRSAGCVQAQPQVRAGSAATPCYQFNSFDPALAQVRYGLGVGFFVQDTWKPTSWLT
                     VVPGLRVDYGTTQNSRAETVQNLLGFGPRLGVNIDLTRDGKTLLKAAYGRANEVVSLL
                     PTFSADATYQSNTWNFNRATGRFDRWVTSSGGGDGYDLRGRCADGSFTTECGNAKLSL
                     TPPHADSVTVSFERELFQNIIGGITYSYRMTSYLWENIELNAVSALDGSKFVQFADPR
                     RGNVYGYRPTAEAYRRYNGVDFVVSGNPSPNWMVFASYTLSFLDGTVDDQMSQLRNDI
                     PRDLRFYGYLSDDHRHQVKLQSSYTFHGLTGGVNLTYLSGAPASRIYRGSKPTSAGYL
                     NRYGWRGLDPAADPNDVRKWTELRNPDLVEINVRMQFDMYELVRQHLSLIVDLFNVLD
                     TSTPIGGSNHAGFENRDIATTFGTVLSRQTPFRAQFGVRFQY"
     gene            complement(44562..45674)
                     /locus_tag="PKL17_00785"
     CDS             complement(44562..45674)
                     /locus_tag="PKL17_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43286.1"
                     /translation="MLPTRAVVSTERYRFPASARLFSVAALLSFAAACSPYPDDGEFL
                     AGVVYAQNFLTGVKTIDRLRAVGKGRGDWPQIPYTVFATTRSADTTAKVSSTGSDGKT
                     VSATTPFWTDGKRDPLAVTNAGQVYVFDGSCQATRDEAFDERLDLIRWDRQYPVFADI
                     PEVLSTSSGKPGRKLNYSAVVEVIHLSAPSSLPCQSIKRFDTATGRIDKDLAVVSREY
                     RLLQIVDPALTPSNAPPARLPVQLGFYNQLVVPYIDMGPVPVSTDGKSFVTMPLYKVY
                     KETAPTATTTPLQVVIPGLPSEVPAGTYSPICKEFVLKGQTSPPPPDANDPAYKAATA
                     SGALASCLPCQTLTVDGSNVPVEVNCPFSQSQVAGK"
     gene            complement(45756..46133)
                     /locus_tag="PKL17_00790"
     CDS             complement(45756..46133)
                     /locus_tag="PKL17_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43287.1"
                     /translation="MSDPERDLRRAVGGPQAVSLVDVELDPERWHRHFITTIGAIGWQ
                     GGPLGPMSLGRVWIDRSLELLGRRLSAPLPSGTRARVTGILFADRDVRSLHKQRVGEP
                     GGYGKSGIYVAQLTAVSIRLELR"
     gene            46348..47160
                     /locus_tag="PKL17_00795"
     CDS             46348..47160
                     /locus_tag="PKL17_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43288.1"
                     /translation="MQTPTVLITGASSGIGASLAKEFARRGARLVLVARRTDRLETLA
                     ADLRTGGTEVLTVTGDVTRDGDMERATEAAEKRFGGVDIVVANAGFGVQGRLTKLSID
                     DVRRQFETNVFGVLRTFYATHQALKASRGRLALIGSVSGFLSAPGTIPYAMSKFAVRA
                     LAEGLDQELAKDGISVTHIAPGFVESEISLVDNQGVLHDTPPRPKAPAFLFMKTADAA
                     RQIVDAIMAREPLRVVTGHGKVLVAASRHTPGLLKGAMALMAGRVAKLTIDD"
     gene            47178..48725
                     /locus_tag="PKL17_00800"
     CDS             47178..48725
                     /locus_tag="PKL17_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK43289.1"
                     /translation="MTAPRIVSARDGELPPKLSQATFDLIVVGSGAGGSMCAQQAAQA
                     GLSVLVLEAGGHHLPSQMNQREGDMLPRLFYDAGGRSTQDGAVMILHGRGIGGSTVHN
                     TNLCKRAPAEVLQQWATSHDCPSWSPERVAPDYEAVETELSVTPLTEEDVNRNNAIMR
                     RGVEKLGWRGGLLQHNRRGCQRSGFCELGCAYDAKENAAKVVIPKALLFGASVICDLR
                     VVRILHDGKRVRGVSASVVRSDGSDGETLNISARAVCVSASAVLSPALLLHSDVPDPH
                     KLVGKSLRLHPGVAVGGCFAEPVEAWKGIPQAYECSEKLSFSDGAQDRCWLIPAFAHP
                     GGFAGLQPGFGKQHAAAMRDYAKTAVVAAMLHDETKGVVSVDGNGRAKVDYALTASDA
                     KALLRGIGAAADILLAAGAQEVMIPFARPLLVRSPKELSVLDSHRYRPLDPILTSVHP
                     MGSLPMGLNPTRAVVDEQGRHHQLHGLYVADGSLFPTSLGGPPQLTIYATGRRVGNAI
                     AADLRHG"
     gene            48739..49299
                     /locus_tag="PKL17_00805"
     CDS             48739..49299
                     /locus_tag="PKL17_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNK43290.1"
                     /translation="MRRMRSGSVFFAALCTLPFFGCAAWHDLFQRDRPTAQIYAEALA
                     LPRPKTTDVAIVLGCPANPDGTPSLCLRCRIHATLSAYRRGEFRAAIFAGGAAHNRFI
                     EAAVMAREAERLGMPRQAILQEGESLTTWQNLRFSRRIMAEHGFQTALLVSTRDHLPR
                     ARRFADYYQIPTALLGCEDEVSPNLK"
     gene            complement(49336..50946)
                     /locus_tag="PKL17_00810"
     CDS             complement(49336..50946)
                     /locus_tag="PKL17_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43291.1"
                     /translation="MSAPSRLIVLSALVCAALLLVVHSLRFNFVTDDAFISFVYAKNL
                     VEHGALVFNIGERVEGYTNFLWTLIVAAGLALKIPAELSSRVLGTLCGILTMFYLATV
                     LRKLRADKESLWDSVSAWILAGIPGYACWSSGGLETQLFALFCTLSLGQYLQAILDEE
                     NGNRAPLWQIGITLGLAALTRPEGYLLFALLALHRIILKIKKRDLIPTKEECQLLLMF
                     LLFTVPHMTWRRWYYGYFVPNTFYIKSSGGKGTWQQGGYYLWAVARDLKLVILPLFYL
                     LGFLRPWPDSVSVRLQKGYVALGGLFFIWVIPFCLYVAKVGGDFMGLYRFMMPVIPFT
                     VLCGAIGLYRLLHRQNRVLLSSVLLGFFGLHAANSYVVDDRALHFIGADRGIDTPGYL
                     RYYTADRAAIGKWLAQHVKPDDYQVVGGAGAQVYYAGIRALDSFGLSDEYVAHNVPAV
                     SNRPGHQKFAPLDYVLSRKPTILTYNVYRISREPYRPSPQEAAEWRARGFHYVSVQIP
                     GLSEPWYSFLKRMDRSLGPLPPASDISP"
     gene            complement(50925..51632)
                     /locus_tag="PKL17_00815"
     CDS             complement(50925..51632)
                     /locus_tag="PKL17_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNK43292.1"
                     /translation="MSRPRPQKTSAIEATIEHLAAAIPLEKPLLRGVSHQAAFFAAFP
                     LAYWLVSLAQTDEARTCALVYGIALIALFGVSALYHRKHWQPGPRAFLKRLDHSNIFL
                     FIAGNYTPITILGVGGSFGKLLCSVLWIGAAAGVLQTVFWPSAPRVLHVGIYVVLGWI
                     GGLGLPGEWRHLGPQVVALHVGGGILYTLGALTYAKKTPNPFPRVFGYHEIFHLLVII
                     ACGALFEVVRKCLLLPA"
     gene            complement(51648..52268)
                     /locus_tag="PKL17_00820"
     CDS             complement(51648..52268)
                     /locus_tag="PKL17_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43293.1"
                     /translation="MRIYFVGSHATGKTTMTRWVSRRYGLPMITEVARSVLAELEIDL
                     DSLRTDMDRVSDYQRRVFERQIAIERLQEKGFVSDRAFDNLAYAADHALVLGDIMSGE
                     SGEEVREYMKWVAQGVVFFVRPHRSLLREDGVRAGVDWESVVRIDGMVKLLLEQFRIP
                     YLPIDTSSMQERVRAVEFVLGRLGLQQQVIEHAPPNRNIVTLVKSA"
     gene            complement(52354..54444)
                     /locus_tag="PKL17_00825"
     CDS             complement(52354..54444)
                     /locus_tag="PKL17_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43294.1"
                     /translation="MLTTRRKLSVLLVLCALLSARFALAAPPNPPPPAKKPDPVAANT
                     VPSQVTLPLAEYQQMLGRPAVTVIETLRIDGSFESRNLSLVLTGRTAGTGPTVEVLST
                     TGGTRLWGCEGDAILSRGGSDKYELTPLLPRFSLRCQVALTSGDRLQLVTTPQVLWID
                     SQVKDGELIRDEEEGGKQRLSVVHIAKDQAEKALPVSAIGRYHVTLSPEGVGFVYQLL
                     LRNPNRSHQRFAIAPHNGERVQRLDAAISCEQQSGEYGCHVPPGEHTLTLRGSLPATT
                     FKPPLQASVHYLLLDGHPLLRPAVESAVQRISVSETGMNPQYRGAQGFLLDDKQTISW
                     RVVRLEAMRTQSYAVSSAATTFFLGSDGHVLGETTLELDNQGAAELQVPMRADPTYAS
                     LQGEPMLLTKNEAGALLLPLSQGAQRVQLQHRQSFSRSVGIGVSTLWLPELPSPAATA
                     SIELRYDRDWLPLYEELSPEVRFPAVSVGELIVLLLLLLGAERLLFALGLSTGLRVSI
                     ALLFCASAAAVSSFLVLFVLAELLVGGLFVLPWLFGQKRLFWLVVGGLCLGGLWALLL
                     GGLLLGGRSADRPAMAPVASEVSYSRKLASDAGGEAAQPEKPKPPVYQGLPPRETIPW
                     GEHRSYLHRERLLSEGRAVQAVMVWKPAVQLVGTAFFALAVLLLSLRAGIVRMGISGR
                     WRRVLEAKAASEGA"
     gene            complement(54469..56073)
                     /locus_tag="PKL17_00830"
     CDS             complement(54469..56073)
                     /locus_tag="PKL17_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK43295.1"
                     /translation="MREFRFGIGAWLGVGVLVGLGLFSPSDALGRHEKRPKASVQDQQ
                     QAQTLVSSGNTALAANNLSAAKTAFEEAFRKNPAPEVLFRIGKLAEAEGRIVAAQDVM
                     RRFLQETGGENDGPDQKDAQRIGQLEAAPSGEVSVIGARGAWVTVDDHLIGALPLQMP
                     LLLEAGNHRIVVEFSDQRIEEQVKSLPGQTVEMRFNQKTGTVVVSVPPALVLIVDGDL
                     TAEQQKALHAAVGKQVLRDRQVVVPGDKAIKLKPALASCLSSASCLAELGLANEALYV
                     LRVKAKKNQAATPDGKANWDFAIDAFDPSVGDYAGRSTASCSDCASEQAFAAAATSVG
                     TVIQQSSARARGSVEITSKPPGADVLIGGEKVGVTPYKGSRFVGDVEVELRLLGQPPF
                     QKTLSVQPGQTAQVEATLAKPDDAAEPAPFVPPPPKAPVYRTEVGPRPKWRLLLGGSL
                     IGAGLVVAGFGVSALSVDGKSTCPGGTCQTIYATQGIGSLLLGIGAASVAGGIVAIVL
                     PGPKRQVQVSSLLAPGLLGLSLRAAY"
     gene            complement(56070..59804)
                     /locus_tag="PKL17_00835"
     CDS             complement(56070..59804)
                     /locus_tag="PKL17_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK43296.1"
                     /translation="MSEETNSPIGQTVGDYKVVKLLGEGGMGEVYAAQHLQLGHRSAV
                     KVLRREIMNNKEAVERFRQEARLIARVRHQNLIDIFDIGELSDGRLYYVMEYLSGQSL
                     SSLLEKKRLSFREVMQIMRQLCAGLSAAHKAGIVHRDLKPDNLFLVEREGEPPLVKLV
                     DFGIAKTVGVEGESMKLTRTGTLVGTPYYMAPEQINGGKVDVRTDLYALGVILYQMCS
                     GQLPFGGDTLGEVLVGHLQKPVPPLPADSVQWGIPAEIGPILAKLLAKAPEERYENLG
                     DVLADLDRLQRGEQTTAGLNSRLTLSAMPKVERVRRRNLVALLLLPVAVCLLAVGGYF
                     FIDKLRSRSKGEEIDMVALRSLALKILQEGLGDGDPQVRGQAVGALEDSRDSRHRSLL
                     EGRLRDADPQVQAQAARALGVIGNRGATAALLHRADEGGDPTVLVSVGESLAKLGAPA
                     GKSLLLRSLEQDRYPQVQLQAALALLDLGEDQAKTLLVTRLGQAKPTDETRVDILSHK
                     ALLGDDEAKTQLIQILPEGPPSQPRQLRVAQILASLGDDRARNLLSDVAVQPGNLQVA
                     AAHALCLIEDPSGQPVLRTTLWQTGAGMPQRLLAAQGIGHCGDKQDAAKLSQGLRLGE
                     RSGILRQAEAGALLKLCDGDPLVLAERSVSWAEQALGDDDWNVRAQAVAALADVNQQK
                     ALPLLRKAMHDNRVEVRRAAADSLARIGGKDAIAALTEGLTDQSAQVRTQMLRAIAKA
                     GQTMGGDAATQGSVMAKLKQDAGQGSASEQVARAAAMVALGDKAQAKEVEKMVGSGDA
                     EAQAMAVEMSDEVPESKQAWLGTIVADEKAPFVTRLKAAVSLARAGSKAGLGILQEAL
                     RRGGAEAIWAQSALTKLGEAGTVELSPKEVQAALKSSDPEVRRSSVEALSGWPAKQAV
                     PKLLDATRDPSELVRSQALDSAMQLAGTDREPAPGLPVLRVLSRDRDPALRERALLAL
                     AKLEKSGVSRATAASKPSEAKATEAKPTPGEKTSEPGKEDKHVDGGAGPEPVPTQPAA
                     VEKGFLRFEGPSGTQVQIDKQPPHTIASKPVEVSVGEHHIVYPGGQQDVTVTAGATVT
                     VKIAPSQLGDLVKAGVEAFQRKDYKKARKLLEKASTLCSRKKEDKAVCQSLGYELSFH
                     LGQVLEAQEAWAQAMTEYDKIQQPGFFGKVKSDGHKAVAEAMKRVAPRVGRLRVSKVV
                     KGRCQTEDIWMPPGRHRVNVGGGQTVQVRPQETVEVKGCP"
     gene            complement(59788..61815)
                     /locus_tag="PKL17_00840"
     CDS             complement(59788..61815)
                     /locus_tag="PKL17_00840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43297.1"
                     /translation="MPEPAVAAASVGPGSILGGKYRLDRVLGSGAMGTVYAAVHEKLG
                     REVAIKVLNAELGQHQEAMARFQREAELVTRLGHPNIVAVYDFGLSPSGAPYFVMEVV
                     TGETLRKRLERGPLSDDEVVSLFAPLLSAVGAAHSAGVVHRDLKPENVMLVPRGDGES
                     PIPQVKLLDFGVAKIRSDDRPENTDGIDAQKLSTLATAVGAMMGTPAYMSPEQIKASP
                     NIDGRADIYAVGVMLFEAVTGQRPFIGDSLASLLGAHLLESAEKPSQIAKRLKLGKRF
                     VDMARLDRVILRTLAKLPEERYADCQSLRDDLDVVWGGRGLWKEASVGQAVTTRPVRL
                     RASRRGLWAWLVPLVLLAGLAVGIVMQTRHHRHVSPYKAGSPPARALDVLQAAERGSP
                     PDKRALLQAIDAVPKRSLLPILTELVADETVPLRPLLATAYVIGQAGDVDLLAAVSKR
                     TQSAVGAQAVEAQAVRLRLGAADAVAVLDSAAASETLPPDARLWAGLALTQAGKAPKV
                     ALQKLKEKLWRPSLSVPRMLRQQLLLELVQKGDETTEKQLREQAAASPLSETALDALE
                     LLALAGKTDAQKDLHELLAKKPPLPQRPLVVLALARARDVGMVDELKPLLSQDTYRLR
                     AVAAAGSLGPKAQSLTEPLLPLLSSSDAVLQKTAAASLLALAEKESHVRGD"
     gene            61940..62755
                     /locus_tag="PKL17_00845"
     CDS             61940..62755
                     /locus_tag="PKL17_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43298.1"
                     /translation="MSKTPQNPTSPPESHYPRDPAARERDPNFWTASHGRAVDPDVLP
                     RVLSRAAALREQAVLVFDLDSTLLDNKPRQARIVREAGQALGEPRLLGCQPEHFTDWS
                     VDKPLRRLGLRDEEIPDLVPKVRRFWKQRFFTSEYCVDDIAIAGAVAFVRDVLAAGSK
                     VAYCTGRHEGMRQGTVACFAREGFPVPDGDRVHLLMKPDLETHDDDFKVRARQPLAAL
                     GTVIAAFDNEPIHINSYFVHFTDAQCVHMLTDDSARGIAPHQSIPSIRDFRRG"
     gene            complement(62756..>65771)
                     /locus_tag="PKL17_00850"
     CDS             complement(62756..>65771)
                     /locus_tag="PKL17_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43299.1"
                     /translation="PFSGEVKVHSRCMLPGCNGSLAGPSQKMMVERGQLLGTLNVGGH
                     SGPFQIEVVSPRGSLRHQFEGSSHVERDMVAAAGGVSYGHKVGVAPYENTVQVPGRDL
                     FVESRKQGSDPFSVSSLIASSGKLSIEVTAKVSAPVLFVHTPNSDGTYAAKQVAVSGD
                     FVPGRKIDVDVAGPYTLVTIGGFVADTFREGWALAFPPSRLKVNLSAQAEGMPKGKVP
                     VTIQAEDRGKGVALSAILEVYDNRVPSRSPATHLHSAVGDSLRNASRSVSSWSDNTGW
                     ELDESDYRAADREEMAESKKEYAPRKMMAAPSMSMGAAPSAPPAPGGLGLSGRGMGGG
                     GMGYGGGLGKMGKKSKNGGGGGGGGEADGGGDEDEAPKEEVREGDKKVVFCQKIVTDA
                     SGRAQVDVVLPPQTGRVVMRVVAVQALEFSQSQRELDVKRVAGAEARLPKTFVPGAEL
                     RIPIDATNATQSTLQLSVTGPGIASEYAVPPGASTHELTVKLSKPGVLALQMKGPDGK
                     VYDRREVSMQTISEQPVTFSRLQFGDGGQISIGADETARVYAGAGPLLRGMAMNVYTT
                     SESWFGHAEALSARVAVRSALLAAVKRGLLSDDGLAHTLRTSVDKDVRDLDEAFCDKG
                     EGLCRPYPGMQASPQWSGWVARNLLAAVRSLQVAASTDPRVVQAQQTAVAMAERIRQS
                     LAAKGQKTEQLAGFDASGQDVVPVEINGQVVYRVITDDAVIRWASDKLLPKLDLDGKD
                     ADVAFAKAYDTFRFLRAFERVGATQYLTELAMAFYQKGDKATFAKLYRQVSRNMILSQ
                     EPGLIQGPALLGGVYSTPMALVRFIELQLLIGAQEATAQGLRKADGSALSFEQTVKGP
                     AQIVVPAAAIARIDRPQIMRLQATTQPFAKVGLSKTQAHVGEELTLEVELAPDRDPLE
                     YYALIAVPTTTSVKQTEDILSDYRGQLIYGQQGQGGTQMQLLTVPFRGKAKLRLLLEG
                     AYKGHAPGLVMIRHIESLSHVCGLAVPDVTVQ"
BASE COUNT        13149 a        20387 c        19322 g        12913 t
ORIGIN      
        1 gcgatcgctc tgacggactt tggtaaggct cagcttgcca aacgacccgc cagtcaggcc
       61 atttacgtat tcatcttccc gataaggcac cgacgctttg ccttcttcgc gtgcggttcc
      121 gtcgaccaac accaagccgg ccagctccga ataacccgcc gtgccggcaa agtcccacgc
      181 ggcatattct tcgacgatcg acgccccgag cgaatgtccg agcaaaaaca gccgtgcccg
      241 tcgctctgcc tgcggaatca agctgaccac ggcgcgcaag tcatcgatct gggtcttgag
      301 cccccactcg gacacgtagg ccagctcgtc ttgcggacga accccttggt aggtacggcc
      361 ttcgacttct tcacctgtga agtaatagcg acgggcaatt tccggatctt gtttgacttc
      421 ggcgacgtcg agcccgtgct gatcttcgag caagttgctg cgccgatcga tcgcccacgc
      481 ctcgatggca tgttcgcccg cgctgcgacg caccatcgcc cgcgccagcg gttcaaaact
      541 gcctgcaccg ccgagaaaac ccggcatcaa cagcaaaatc gcccgcgctg gtttcggtgg
      601 tgcatcgcca gtgtcgacgc gatagcgcac cacgcgcaca aagttgaact gggccggggt
      661 ttggccgccg cttgaaaccg ccggatttgg cgccggggcc ggcgctgaaa ccaggaaaat
      721 ttcccgtctg atcgccacct gtccttgctc caccaccgtc gaggtcggag gcggcacata
      781 gatcgcttcg ggttccgggg tggccgtgca agccgaaagg caaaccgcga gcagaactga
      841 aaaaattgac tttttggcgg gctgaaggtg ggccatggtg ggcagacctt acctgcactc
      901 tctctcgctg agcaaccctc gctgcgccca gccctttacg aactgcgcag tggagtgcta
      961 aaagggcggc acattggttg tgcctacatg tagtcgtttg ggtgacgcgg gccgaagcgt
     1021 tgcgtgtttg ggtggtgacg acgtggagac ggggtactaa aaatgctctt cgactgggtt
     1081 tacgggctgt tttcgaatga ccttgccatt gacttgggta cagcgacgac gctgaccttc
     1141 gtcaaaggcc gcggcatcgt ctccaatgaa ccgtcggtgg tggctgtgca gcgatcgtcg
     1201 gcgggcagca agcgcgtgct cgcggttggc aaggaagcca aggaaatgct cggacgcacg
     1261 cccggcaaca tcgtggcggt ccgaccgatg aaagacggcg tcatcgccga ctttgaagtc
     1321 accgaagcga tgctgcgcta cttcatcatg cgggcgcaca accgccggac gctggtcaag
     1381 ccgcgcatca tcatctgtgt tccgtcgggc atcaccgaag tcgaaaagcg ggcggtgcgt
     1441 gactcggcgc tcgctgcggg ggcccgcgag gtgtacctga tcgaagagcc gatggccgcc
     1501 gcgatcggtg ctggactgcc gattaccgag ccgtcgggga acatgatcgt cgacatcggc
     1561 ggcggcacca ccgaagtggc cgtcatttcg ctcgctggaa ttgtcgtcag caagtcgatt
     1621 cgcgtcggtg gcgacaagct cgacgaagcg atcgttcagc acatcaaacg caagtacaac
     1681 ctcttgatcg gcgagcgcac cgccgaagac atcaaaaagc aaatcggttc ggcttacccg
     1741 ctcgatgaag tgctgacgat ggatgtcaaa ggccgcgatc tcgtcgctgg tgtgcccaag
     1801 acgctgaaca tcaattccga cgaaattcgc gatgcgttgg ccgaaccagt caatgccatc
     1861 gtcgaagcgg tgcgggtggt gctggaacga accccgcccg agctttcggc agacatcgtc
     1921 gacaaaggca ttgtgctgtc gggtggtgga tcgcagctca aagggctcga tctgctgctg
     1981 cgcgacgaaa cggggctgcc ggtggtggtc tcggagacgc cgcagctggc ggtggtgctc
     2041 ggttctggca aggcgctcga cgagctgttg ctgctgcgcg aagtgaccgt ccagtaaggt
     2101 gccggtttgt cgcttttccg acgagccaaa ggcctgctga tcggcgcggc gctgctgctt
     2161 gcgctgtcgc ttgtgctgcg catgagcgtg cgctcgccgg aaaagcagac gctcctcgat
     2221 cgcgccgtgg tgcggttgct tgcacctttg caggggctgt cggcggggct gcttggtcga
     2281 atttcgtcgc tgtggaccgg ctatgtggcg ctcgtcgggg tgcagcaaga aaacctggcg
     2341 ctcaaaaaac aaaacgccga gctgcgctcg cagctcacga gcgtgtcggc gctggcgatg
     2401 cgggcgcagc ggctggaacg actgctggat ctgcgcgccc aagtgctggc cgataccgcg
     2461 gtggcgcagg tcatttccgt cgaaacgtcg cgtcagttcc gggtcgtgcg ggtgcggatc
     2521 gatcgtggcg gcgtcgaagt aaagccaggg atgccggtca tcgctgcgga gggcgtggtc
     2581 ggtcgcatcg cgcgcagcgt cggcccgtat gcggatgtgc agcttctcac cgacgcgcga
     2641 tcggcgattg acgtggtgct gccgcgcagc gaaagccgcg gcgtcatgaa aggcaccggc
     2701 agcgacagcc gctacgttag tcgcatcgaa tatgtgctga aaaaagagtc gatcgaagtc
     2761 ggtgccgacg tgctgtcgtc cggcctgggc ggcagctttc cgcgcgatct tcccgtcgga
     2821 aaagtcacgg cggtgcgcaa aagcgacgcc agcttgtacc aagaaatcga agtcgaaccg
     2881 agcgtgtcgt ttgcccacct gcgcgaagtg ctgattgtgc tgtcgccgcc gccaccgccc
     2941 gatcccgacg ctggcaagcg cggttttgaa gcagcacgcg gccccggagt tccgcggtga
     3001 acggctcgcg agcgatcgga ctgtggcttt tggcggtcct tggctcggtg ctgtcgacgg
     3061 gactttcacc gctgacgagc tttgcgctgc cagattttgc gctgctggtg gcgctttatg
     3121 ccggtctcac cagccggcac agcggcgggg tggtggcggc gcagcgtgac gcatcggcgc
     3181 tggggatgct ttctctcggt gcagcgatgg gctatgcctc ggatctggtc gaaggaacgc
     3241 cgcacggtct gcgcgcgctg ggaatggcgc tgctgctttt gattttgcac cgcgttgcgg
     3301 gtcatatgct cgtcggaggc cgagccgccc aaggcgcggt ggcagcgctg gcgcttctgc
     3361 tgttccgctt gcttttgacc ttgatcatgc tttttgccga cggggatcgc gatctgttgg
     3421 tgagcggact gcgcagcctg ccgatgcagc tcgtgctgac cgcaggggtg gcgccgctgg
     3481 ccttatctgt gctaagccgc atcgacgctc ggctgcggtc caaagcgacc gggccggggc
     3541 tgtggatatg aaactgttcc gtcgacaaaa actgcacttc gacctgcgca ttggcattgg
     3601 gtcgtcgctt gcaccatcgg gcgtcaccgc cggacaggcg cagacggcat cgctgcgagc
     3661 gccgctgccg gtccgggtcg cggcggtttt gtcggtgttg gtctttgtcg gactggcggg
     3721 acggctggtg cagcttcagg ccctgcgcgg cgacgactat ctgcaaaaaa gcaccagcaa
     3781 cttcattcgc gagcgcgagc ttccgtcggt gcgtgggcag attcgcgatc gccgcggcga
     3841 ggtgctggcc gacaaccgac cgacgttcgc ggtctatgcg acgccgcgtt acgtatcgga
     3901 agcgacgctt ttgcggcttg gacggctgct gaagctgacg gaagaacagc gagaggcgct
     3961 ttcaaaaaaa ctgacggtgc gacgcggtgc cgaagcgagc aaacaactgc tcgtcgccga
     4021 agacatcacc cgcgatcaac tggcggcact ggaatcggaa aaagatcagc tcccaggcat
     4081 cgacatcgaa gcgcgtaccc gacgggtgta tccgcagaaa cagctcgcgg cgcacttgct
     4141 cggctatctg aacatggcgg ggcccgacga cctgaagcac ggctacaaac acggcgatta
     4201 cgtcgggcga tcgggaattg agcagaagct ggaacaaaag ctgcgaggca gccccggttt
     4261 cgagcggatc ttcgtcgacg cgcacggtcg cagaaagagc gaacacgagc tcggcgagat
     4321 ggcgcagctt gccccgcaag aaatgcgacg cgagccacag ccggggataa acgcagtgct
     4381 gacgattgat ttgcagcttc agcgcatcgt cgaagaagcg ctgtctaggc atgcgtcagc
     4441 agcggcggcg gtggtcgaag cagacagcgg acgggtcttg gcgctggcgt cgtatccaag
     4501 tcccgatccc aacctgctga gcgggcggct gagtcgtggc gaagcgcagc gaatggagag
     4561 cgatccgttt cggcccctgc tcgacaaagc gctgcgcgag acgtactacc ccggatcgac
     4621 gtttaagatc atcccggcgc tggcggcgct cgaggaaggg ctggtcgatc ccgatcaaaa
     4681 gcaggtctgc cacggacgct atgagctggg gcgacacgtc ttccgctgca tgaaaacgca
     4741 cggtccggtg tcgctccacg aagcgctggc gcagtcgtgc aacgtctatt tttatcacct
     4801 ggcggaaaag gtcggactgg aacggatggc aaactgggcc gaccggtttg gtttcggtca
     4861 gccgacgggg cttggacttg gtgaagcgag cggttttgtg ccgacgctgg agtattacaa
     4921 acagcacggt ggcttccgcg gcggctatgc gctcaacacg gcgcttgggc aaggggcggt
     4981 gcgtgcgtcg gtgctccagc tcgcgatggc gtatgcggcg cttggcaatg gcggcaagct
     5041 gtatcagccg tacatcgtcg aacggctcga aaccccgtcg ggtgaaccag tggaacgctt
     5101 ttcgccccag ctgcgcagtc agcttccgat tctgccgtcg tcgctggaac gactgcggcg
     5161 agcgctggtc gacgtggttg cggacaacaa aggcaccgcc agcaccgcgt ttattgatgg
     5221 tctcgatatc gccggaaagt cgggcacagc gcaggtccgc aaaaaccgcc ggggcgaatc
     5281 gagcggctgg gaccagctaa acgaccatgc gtggtttgtc ggttatgccc cgtcgcgcaa
     5341 tgccaaaatc gccgtcgccg tgctggttga acacggcgga ctgggtggac acatcgcagc
     5401 cccgacggcc accaagattg tgcaaggcta ctttgaccgc gtcgcgccgg agctaaagcc
     5461 gcgcccggtt gccgatgtca gcacgcccaa gctgcgcacc acgttgcctg cctctgccgg
     5521 agcccgctag ccgcgatgtc gatgctgcgt cgaacctggt cgctgattga ctggccactg
     5581 accgcggtgg tgctgacgct gattgggctg gggctcttga atctgtattc ggcgacgcgc
     5641 gtggccccca aagggatgtt ttcgtcccag cttctgtgga ttgccgtcgg aaccctgatt
     5701 ctgattgcag tggcggcgtt cgatccaaag tggctgcggc ggctggcctg gcctgcgtat
     5761 ctgggcgtga ccggactgct tgttgcggtg ctgctggtcg gaaaagtggt caatggctcg
     5821 cggcgctggt ttggctgggg ttcgtatggg attcagcctt cggaactggc caagctgtcg
     5881 cttgtcttgc tcggtgccca tctgttttcg agcgatccgc aaggcatgac ccacaagccg
     5941 tggcactacg cgatgaagtg gcatgcgctg tgggcgctgc cggcgctgct catcctcaag
     6001 caaccggact tgggaacggc gcttttgtgc ggtctgctcg cgctgtcgct gttttttttg
     6061 gtgccgctgc cggccaagct caaaggctgg gtgctcaccg tcggtctgtc cgcgacggtg
     6121 ctggtgatgc gttttgggct gaaggaatac cagaaaaagc gcctgctcac gtttctgcat
     6181 cccgagctgg accccaccgg aaccggctac cacgcgcggc aagcgctgtt cgccatcggg
     6241 tcaggaaagt tctttggcaa agggtatctg cgcggcaccc agaatcactt gcagtttctg
     6301 cccgagcact ggaccgactt tccgttcgcg gtctgggccg aagagtgggg acttttcggg
     6361 tgtctggcga tgctttcgtg tttcctgttt ctgattctgt ggacactgca cattgccgcg
     6421 tcctgtcgcg atcggttcgg tagttatctg gtgctgggct gcgcggcgct tctgttttgg
     6481 cacgtcagca tcaacatcgc gatggtcatc ggaatggctc cggtcgtcgg tgtgacgctt
     6541 ccgcttattt cctatggtgg gtcgtcggtg ctgaccgtga tggcggcgct tgggctcatc
     6601 tacaacgtct ctgctcggcg cttttcctac taaacggagt ccctttgtcg acgcactttg
     6661 tccccatgac ccccgcgctt gaggagtacc tcaccagtca ttcgacccgg cgtgatgacg
     6721 tactttctgc gctggcccaa gagaccgcac agcttggcga cgtcagtgtg atgcagattg
     6781 ccgagctgca aggatcgctt ctgtcccttt tggtacgcgc gacttcagcg gtgcgcgcga
     6841 tcgaagtagg aaccttcacg ggttattccg ccatctgtgt ggcgcgggcg ctgccgcccc
     6901 atggcaagct gctctgttgc gatgtcagcg acgagtggac ccagatcgca cgccgctatt
     6961 ttgcgcaggc gaaagtcgaa gacaaaatcg atcttcagat cggtccggcg ctcgaaacgc
     7021 tgcgaagatt gccgcaagag ccgctgtttg acatcggatt tatcgacgcc gacaaagtca
     7081 atcagcaaaa ctactacgaa gaggttctca cgcggcttcg cagcggcgga ctcttgctca
     7141 tcgacaatgt gctgcgtgat ggcagggtga cggacccaac acagtctgat ccgtcgctgg
     7201 attcggtgcg ggcttgcaac gacgccgtgg cccgcgatcc gcgcgtcgaa gtcgtgatgc
     7261 tgccgattgc cgacggactt tcgctgattc gcaagcgcta gcccgcttcg ccgcagccga
     7321 cgcatcagcc cgttttggca gaccgcccgg tctgatatca tgaagtctcc gatgaaacgg
     7381 agtctggcga tcggtttgct attcgtcgcg gcttgtccgg aaaccaaagt gtccggactt
     7441 gggcaggctc ccatcgaagc aagcagtgga atcgatgcca ccgacgagcc gatttctccc
     7501 ctgccgattt ctttgccgct gtctgctgaa aaaatcgcgc tgggggaact gctgtttgct
     7561 tcgccgctct tgtcggcaga tggatcgctg agctgccttg gctgtcatcc acttgatcgg
     7621 ggtggcaccg acggaaaaat ctattcccaa ggcgtccatg gcaagctcgc tgcggtcaac
     7681 accccaacca ttttcaacgt cggctttgcc ttccgactca actggaatgg cgcattttcc
     7741 agcctggaag cacagctcag tcgcccgctc gaagtcctga tgggaagcag catcagcgac
     7801 gtgctcacac gaattcgcag cgacaaaaac ctgagccgcc gcttttccga catctatccg
     7861 cagggcgtta cagagaccaa cttccgcgat gcgatcgcga cttatgaacg atcgcttttt
     7921 actcccaatg cgcgatttga tcgatatttg cgcggcgaaa aaaatgcgat cagcgacgaa
     7981 gaattgcagg gttacaagct gtttaaggaa tacggctgcg taagctgtca tcaaggccaa
     8041 aacgtcggcg gcaacatgtt ccagaagttt ggcgtgatgg gggattactt cgccgatcga
     8101 aagaaagcga ttctggatgg cgaacaggga cgcttcgaag tgacgaaaaa ggaatccgat
     8161 cgctatgtct ttcgggttcc gagcctgcgc aacgtcgcgc tctccgcacc gtactttcat
     8221 gatgggtcgg ccccgacgct ggaagccgcg atttcagtga tggcgcgtta tcagctcggg
     8281 cgcagcattc cgcagccgca tgtgatcaaa ctcgcagcat ttttgcgcac gctcaccggg
     8341 gaatttcgag gcaagccctt gcaatgatcg agcagaacaa agactcccag agcttgcccg
     8401 atgcaatctc tgtgggattc ccaaaagcct ttgggagtcc ggtgtcgacc gtgattctgc
     8461 ttttgatcgt gctgtgtggt ctggttttga cagcgctggt ggtgacggca aaaaagtaca
     8521 cggtggatgc gcggctgcat gaaaagtatt ccggatcgct gcgcagtctg atcgcactcg
     8581 acgcacaact gaatgaagcg gtgctgaaga ccagcagcgg aatgtctgtc agctacgatc
     8641 cgctccagcg tcttttgcga cagcttcaca gccagagcgt cgaactgcaa aacaccactt
     8701 ggccgattcc ctattttcca caggaaggat cggtttccgt cgaaaaatcg ctgacggaat
     8761 ttctgtctct gctggctgac aaagaagtcc tgatcgaaaa attccagacc gaaacgtcgg
     8821 tggttcgcaa ttcccacaac tactttccgg tcggagtccg ccagcttgtg gacgcgcgac
     8881 aaaaggaatc gaaacacgat ccgctgtctt cgtcgctgca tgagcttctg cagtgcgtgt
     8941 ccctttatac gctgctacca aatccagaac attctgtagc gtgtctgcac acgctggcat
     9001 cgcttcagca gaatctccat gtgctttccg gaaccgatca gcaagaagtc cggctgctct
     9061 tgctgcacgc gcaggtgatt ctggatcgcg aacagagact gtccgaagcc gtgcgcagcg
     9121 tgctttcgct caagactcgc gatgtcgccg accatgcacg caaacgctac gacgatcagt
     9181 atcaagtggc gctgcgacgc ggcgatgtct atcggctgtc cgcagccggt ttttttgcgc
     9241 tgctcattgt ccttctcgcc agtctgctca tccacaagct gcggcgctcc gcggcagcgc
     9301 ttcagcatga acgagagctt tcggaacagc tgctgctcaa cgtcctgccc aagccgattg
     9361 ccgatcgttt gaaacagaca ccggaagtca tcgccgatag ttttccgacg gtgacggtgc
     9421 tgtttgccga tctggtcggt ttttcccagc tatccgccaa gctgccagcc attgatctgg
     9481 tgcgactgct caaccggatt ttttctgcgc ttgatctgct ggcgcaaaag cacggtctgg
     9541 aaaaaatcaa gacgattggc gacgcataca tggtggtggc cggactcccc atcgcgcgtg
     9601 cagatcatgc agaagcgatg gcggaatttg ccctgtctgc catcagcacg ctcgattctc
     9661 tgcgcagcga gctgccgcaa gacatgtcat tccgcatcgg catcaacagc ggttctgtcg
     9721 tcgctggggt gatcggtctg cgcaagtttt cctatgactt gtggggacac accgtaaacc
     9781 tggcaagccg gatggaaatg cacggaattc ccggtgcgat ccactgcgca gaatcgacgt
     9841 atgagctgct caagaaccga tacgaatttg tgccgcgcgg accaatcccg atcaaaggaa
     9901 tcggtcccca gacaacgtat cttttgcaaa aacggaaggc tgcggaccca gcggcttagc
     9961 gcggcgtgat gatcttggtg tcaggcgggg gcgtaaacga aaagcgcgcg tcatcaacgc
    10021 cggctttggg attcggttcg attcccgaaa acaccaggtg gttttgtccg ccttgctgat
    10081 cgaacagcac cgtttctttg accaggaaag tcttcggctg cacgacgaac agcaggtggc
    10141 gatactgcgc ggaagcgacc ttgggcagaa gcttcagcac cagatcgccc gcttccccga
    10201 gcggctgatc gtcgaaatag gaaatctcga actcttcttg cagcttgccg cgaccaaaca
    10261 aaaagctgac ctgcgccggt agctgcgatg agctgagcga ctgcttgtac gcctgctcgt
    10321 cgtctggttc gtagacccac agcgtcgttc cgtcggcaac gaacagcttt ttttccggct
    10381 tttcatagtc ccagcgcatc ttgccgggct ttttcagcgc caccgtgccc gatgcctgtc
    10441 gtttgcccat cggcccggtg agcgtctgcg aaaacttcgc ccgcaacgac gcaatgcttt
    10501 cgtaactttt ttgaagctgg ctgacgactt cggcgacggg aggacgggct gggatcgccg
    10561 catcggtcgc tttgcgcggc gtggtcgctg gggtcgcagc gggatttttc gcggcagtgg
    10621 ctgctggagt cgccgtcgga gcaggcggag caggcggagc agcggcaatc gaaaggacaa
    10681 acggcaggac ggacagtttc ggcagaaagc gcctcatcgg gttctccaca ggtggatatt
    10741 cgcacaaaga cagatgcgac gctgctcggc tctattcaaa ttgccgcacg ctgtggggac
    10801 gatgcagcaa aaactaatga gcggcggttt tggctagcgc gtctttgacg aactgttcga
    10861 tcttcggctt gggcgcggca ccgacgagct ggccgatgac tttgcctttg tgaaagacga
    10921 gcagcgtcgg aatcgagcgc acatcgtact tggtcggggt gctcgggctg tggtcgatgt
    10981 ccatcttgcc gacgtttagc ttgccttcaa actgggtggc gagcgactcg acagcggggg
    11041 cgatcgcccg gcagggtgcg caccaggtgg cccagaagtc gaccagcgtc ggtttgtcag
    11101 aggaaagcac ctcggcctgg aagttggcgt cggtgaggat cttgatgtgg tgcgatgaca
    11161 tgggattctc cttcagccac aatctaatat gactggccgg ctgcgaaagg ggcgaccgct
    11221 ttttttcggc tattgtgcgg gccgcccatg agcctagacc gaaccgaaac agcgcagcca
    11281 cttcttccgc ttccgcagct tcccaagctg ccgctgctcg acggtcaggt tcaggttgac
    11341 ggcattgtgt cgagccaggt gttcgtgtcg cccgaagcag actttgccgt gctgcggctt
    11401 gatgtcgcgg gacagattcg acccgtgatt gccgtcggtg cgctggccgg tctgcgaatc
    11461 ggtgagacgg tgcgcatcat cggtcgttac gaacagcacg accgctacgg tcaaaggctg
    11521 cgggccgatc aagcgctgcc gcagacgccc gagtcccggc tgggtgtcga gcgctatttg
    11581 tcgacgctgg ctggtctggg tccagagctt gcgcgccgca tcgtcgccga gcttggaccc
    11641 cgcgcgctca tcgccctgga agaagagacg tttcgcgttg cccagatcaa aggggtcgga
    11701 aaaaaacggg cccagcgggc gctgattgac gctcgggctc gtcgagaaga acgcgaagtg
    11761 atgatctttt tgcaggggct cgggatttcg gcagcgtatg cggcgcggat tcgcaagcag
    11821 tggggacaga cggcgatcac caaagtgcgc gaaaatccct acgcgctcgc gcgggaagtc
    11881 accggcatcg gttttcacat tgccgatcgg attgccgccg cgatgggcgt cgagccgcta
    11941 tcacccttgc gctgtgtcgc cgggggaagg catgtgcttc tcagcgcctg tgactcgggg
    12001 cattgctatt tgccgacgaa agagctgctg cagcgagcgg tgcagctgct cacgcaagcg
    12061 ccgcccgacg atcacgagcc caatcgacca ctgccgccac ccgacgaaaa actggtcgat
    12121 gcggtggaaa aatcgatcga gacgatgcgc tcgcacgggg aaaccgtgca cgaaggtgat
    12181 gcggtctatc tttcgtcgct gcaccagcaa gaagttgacc tgacgcggca ggtgtcgcgg
    12241 cttctgtcga cgatccgtgc gcgaattccg accatttcgg aacacgacat ctcgctgcgg
    12301 ctggcagaag gtcagaaagc agcgcttctg cgggttgaaa cgtcgccgct tagcatcatc
    12361 acgggcgggc cgggaacggg taagacgacg atcatttcgg cgctggtgcg ggcgtatctg
    12421 cgcgcggggc tgcgggtttg cctgactgca ccgacgggac gagcggccaa gcgactgtcg
    12481 gatgcgacgg gccagaccgc caagacgatt caccgcctgc tcaacctgat tcccggcgag
    12541 cgcgggcggg tggccaaaga cctggaaatt gatctgctgg tctgtgatga agcgtcgatg
    12601 ctcgatctgc cgctggcgct gacgcttttg cgagcagtgc ccggtggtgc gacagtggtt
    12661 ctagtcggcg acgtcgatca gctcccgtcg gtgggaccgg gtcgtgtgct ggccgatttg
    12721 attgattcaa cctgcattcc ggtgacccgg ctgcgcgaaa ttttccgtca aaaagaaggt
    12781 tcgggaatca tcgacaacgc gcaagcgatc ctgcgcggcg agctgcctct gtcggcacct
    12841 tccgacggaa aattggccga cttttactgg atcgaagtcg acgatgcgca aaaatcgcgc
    12901 gacacggtgg tcaagctggt gtcggaacgg attccgcagc gctttggttt tgacccgctc
    12961 accgacgtgc aggtattgac gccaatgcat cgcggtgaag tcggaaccga agaactaaac
    13021 cgcgcactgc aagcccagct caatccagcc gccaaacagc cgcagcgccc gcaagcgacg
    13081 acgggagagc ccacagccga caagccaagc acgccgcaga aagtctcgtt ttacgtcggt
    13141 gacaaagtga tgcaggtgcg gaacaactac gagctcgacg tctggaacgg cgacatcggc
    13201 gtgatcatcg ggcgcgatga agaagacgac cttgtgctgg tgcggtttga agacgagcgg
    13261 caggtcgcct atgacgccga agcgcgcgat gatctggagc tggcgtatgc cgtcagcgtc
    13321 cacaaaagcc aaggcagcga gtacaaagcc gtcgtggtgc cactgcacat ccagcacttt
    13381 ccgcttctgc gacggaattt ggtctacacc gcagtgacac gcggcaaaaa actggtcgtg
    13441 ctggtcggca gtcgtcgggc ggtacggcgc gcggtgaccc aaaccggcga tctccagcgc
    13501 tacactggtc tgcgcgagcg gcttcgcagt atgctgccaa gcggatgaac cacttcccca
    13561 acacctcgtc gagaaactgg gcgtatgtcg cagtgctgct ggcggcggtg gtttcgacgt
    13621 gggcctgtct tgcggcaccg ctcgacctgg gtttcggtgc aaaaggcgac gctgcggtgg
    13681 acagtggggt ctatcccgac gacggccaag ggcaaggcgt gctcgggctt tttaccatca
    13741 ccggctgtgg caagctgacg ttccccggcg acgagccacg ctgcgagggt ctttcgcccc
    13801 tttcgcttca gcttgtactt ctgcccattg gcgtgtcgag ctatcgctgg caggtggtgc
    13861 cggcaaacgg aaccagcgac ggtggagtaa gtgatggcgg acttggttcg ctgctcgatg
    13921 aaacgcagag ccgcagccgc agtcccagct tcaccctgac gcgacctggc acctattatg
    13981 tcaatcttgc cgtcgctgga cccggtggca ccagcagcgc cagcggccag atcatcgtcg
    14041 gcacgggcga tatcggagcc ccctgtcgca gcgacgatca gtgccaaaac ggtctgcgct
    14101 gcctgtgcgc ggctgacaag cctggtcgcg atggcgcctg tccgggctcg ctcgcaagcg
    14161 gactttgcac caaaagctgc gacggtgtcg cctgtcccac cggcagcgtg tgtctgaact
    14221 tgtcgcgcag ccaaaccgca cttcccgacg gtggcagcgg cgatggtttc cgtcagccga
    14281 tctgcgtcat gccttgcagc gtcgacaccg actgtcgcgc cgaccaagtc tgtcgcgagc
    14341 taccccagag caaacccggc gctgcggcat cggccccgct tcagtttggc aaagtctgtt
    14401 ttgcgtccat ccccgcgggt gtcggtgcaa gctgcatcgg ccctgacgaa caaccaagcc
    14461 agagcgactg tgcgaccggc atctgcgaag cgctcggttc acacgatctg tgcaccattt
    14521 cctgcggcgc gggctgtcca cagtctgccg cgtgtgcgac gtggaatggc accgtcgccc
    14581 ccgtcccgag cggtccgcgc tgcctgggcc gctgcgatgc aagccatccg tgtgcagatc
    14641 cactgctcga ctgtttgccc ggtggtggca gcggcggtct tggctttacc ctggtcggcg
    14701 aaccgatgat cacccaagtc tgcgcgccgc gtcgctgtag caaagccgcc gactgtccgg
    14761 gcggacgctg cgtcaccatc ggaacggcgt cattctgcct gcgcagttag ggggcccaag
    14821 cccgcgccgg ctttctcttt tggcgacagc gcgcgaaatg ccgcaatcag cgcggccatt
    14881 ccctgcgcaa tggatgtcgt cgctgcaaag ccaagcagtt ccctggcccg ctgtggcgaa
    14941 ccgacaaact gcgtcacatc gtagctgcgc gcgggcgctt cgacgacgcg gcagcgccca
    15001 ccgcccgttt tttgcgccaa gtcggccagc tgcgaaagcg tggtgccctg cccggtcaca
    15061 agctgaatcg gcggcaacac cacaccacac gccagccgat cgatcgtggc caagatgccg
    15121 cgcaccgtgt cggcaagatg ggtgaagtca aaaacgtgcg aagatccatc gacgcgcaaa
    15181 agtccgcccc acagcgctgc acgcgcaaac gccggcacca ctcgatcggc atgatcgtgg
    15241 gtgctgccgt acacattgga aaagcgcagc accgctgtgc caagtcccag cccgcgcgcc
    15301 gcaaagaccc gcgcttccgc ttcggctttg gaacgcccat agatattgag aggccgcagc
    15361 gcggtgtctt ccgtgacggg aagctggtca acctggccgt acacttcgcg gctgctcgaa
    15421 aacagcagcc agggacgatg ggcctgcgcc acacacgcat cgatcacttc ttgcgtgccc
    15481 agcacattgg tatcccagca cagcgttgga tcacgctcgc ccaagagaac ccgcgaaacc
    15541 gccgccaggt gcacgacgcc atcacagccc gcaaccagtc gacggaccga atcccgaatc
    15601 cgaacatcac ctgaacccgc gtgacctatc ggcaaacaga catcgagccg gcgcaccgcg
    15661 tcgccgcgat cgacaagcgc ttgcgcgagc gccgtcccga tgagtccttc cgatccagtg
    15721 atgccaatga tcgccatcgc gtctgcttgc tgccgccggg tttgggcctt ccgtcaaaag
    15781 gcaaaccatc aaatcttgcc acgaaacggc atgcgccaac gtgataagat gtgaaggtgt
    15841 ctactcttca gaagcggctt ctttcccccc gtagaaacgc aacctccgct aggcttcatc
    15901 ttgcactcct tttgtcgatt ccgattgtcg ctagcgcgtg ctcgcagcca cttgtgattc
    15961 ggtccgagcg cacacaagcg atgatgccga ttgccaaaag caagacggtc gtctttgtgc
    16021 acggcatgtt tatgacgccg cttgcatggg aaaagtggga agcgcacttc gccgccaagg
    16081 gctataagac gctcgctccg gcgtggccgc tgcacgatct gccgattgaa gatcagcgca
    16141 aaaatcatcc acatcccaag ctcggtgagc tgacgctcga cacggtgctt tcacattatc
    16201 ggcaggtgtt aaaaggtctc gatgaaaagc cgatcttggt cggacactcg atgggcggac
    16261 tcatcgtcca gatcctgctt gccgaaggcc tgggagccgc aggaatcgcg attgattcgg
    16321 cgccgcccaa aggtgtgatc tcgctcaaat actcgttcct aaaaagcaac tggccggtga
    16381 taagtccgag cgcaaaagtc agcgagccgt ttttcccaac ccaacagaat ttcgactaca
    16441 gcttttccaa ctgcattcct gccgcagaac agaaatccgc ttacgaacgc ttcgccgttc
    16501 cagaatcgcg ccgggtgggc aaagccccaa ccgaagatgt tgcaaagatc aatttccaga
    16561 cggcacgacc accgcttttg ctgatcgctg ggagtcttga tcacatcatt ccggcgagtc
    16621 tgaatcgatc gaactacgac aagtacgatc gcagtccatc gatcaccgat ttccgcgagt
    16681 ttgaaggacg ctgccacttc atcatcggcc aaaccggctg ggaggaagtc gcggactatg
    16741 tgctcgactg gattgccaaa aaccagtagc aaaaaaacag cgcacgcttg accgcaccga
    16801 catccaaaca ctgtctcctg ctctttccca aaaaggacgt cgcatgaccg ccgccgcgtc
    16861 tgcccttccg cttctgacca aagaggagtg tctggcgcag cacccctttc ccaaggaatt
    16921 tgccgccgca aagcgcctgg agtggttctg gcattacgac gttgatctgt cggcaaaaga
    16981 gttgtggacg ctggtggcgg attcgtcccg gatgaatcgc gcgctgcaag taagcgagat
    17041 gaagttcgag gatcgcaacg ggcagcgtat tggcagctcg cgaccgggcg gattttttca
    17101 tgagtggcag gaagtgccgt ggaactgggt ggcggaacga ttcatcgagt ctgtccgtat
    17161 ctaccgcagc ggtttttcca aagtggtcta cgccgtgttc atgcttcagc cgctctcgac
    17221 ggaaaaaacc cgtctgtatg tctattttgg cgcggttccc aaaaaccccc tcaccagtct
    17281 ggcactgcgt ttgggattcc cttcggtggg tcgcgaatat gcaaagcttt tgccacggct
    17341 cgctgatgaa gtgcgcgaac gtcgaccgct gtctttggta cagcctggcc ccggactcag
    17401 cgaagcggcg cagcagcgac ttgcgacaat ccgtcaggac ttgatcgcaa aaggactcga
    17461 tgtccgcgcg gtcaaccagc tcctcgattg gattgcaaac ggcgatgaac aggagctgtt
    17521 ccgcatccaa gttcgcgagc gcgcgcgcgc ctttcaggtc gacgaagatg cgctgcttcg
    17581 ggtgtgtctg catgcgacgc gattgggact tttggaacta agctgggacg tcatgtgtcc
    17641 gcactgccgc ggtgtcagca aacagaacgg cagtctgggt gacataagta cgcaaggggc
    17701 ctgcgaagtc tgtcttatcg actttggtac cgactcaagt gatgcagtcg agatcacgtt
    17761 tcatgtccat ccatcgatcc gcgaggtgcc gctgcggacg ttctgtagtg cggaaccagc
    17821 gacgaaagaa cacatccggg tgcagcacag tgtccccccc gatggcaagc agattctctt
    17881 gccgccgctt cccaaaggac gctatcggct gcgctggcaa ggacacaagc gctacggcta
    17941 cctggatgtg ggggtgccaa gcggtgcgcg tgaaatcgac tggcggacaa gcagccagcc
    18001 atgcgagctt tccgtcgaca gtgactttgc gctgcagctt cacaacgaca gctccgaaac
    18061 cgctctgttt gtgctggaga ccgcacagtg gagcgacttg gcactgcgac ctggacgcat
    18121 ttttagtctt caggaattcc gtgatctgtt tcgggaagag tatctaagcg ccgatgttca
    18181 gcttgcgatt ggacaacaga cgattctgtt tacggacatg gttggatcga cggagcttta
    18241 cgcaaaacag ggtgatgcgg tggcattccg tgaagtccgc cgccattttg ccgatgtctt
    18301 ccgcatcatc gcggaacatc gtggcgccgt ggtcaaaaca atcggcgatg cggtcatggg
    18361 cgcgtttacc agtccgctcg atgccgtcct gtccgccaag gccattcacg actgttttca
    18421 ccccggacgg agcgactcac tgaccaagct gcgcatctcg gtgaacgtcg gtccgtgcat
    18481 cgcggtcaag ctcaacaccg acattgatta ttttggacac accgtcaatg tcgcggccaa
    18541 actacaaagc ctggccgggg cctggcaagt ggcgctttcc gatgaagtgt attttgcccc
    18601 tggcgtcgct gactggctgg caaaagagcc tgcaaaacta gaagcagtcc gttacgaatc
    18661 tccttcactc ctttcgccga tcagcggcaa gcgctggacg gtctacgaag gaggcccgta
    18721 ggagcgaaga agttcagaaa cggactgctt agggggacga ctgggagtct attgtccgac
    18781 ggacacgttc acttttcggg gctgtgcggc agtgcgcttg ggaatcacaa tgctgagcac
    18841 acccgcgttc agatccgctt tgacctgatc cacatcggcg ctttccggta gcgtgaacgt
    18901 gcgctggaaa gaaccaaagc tgcgctcgac catgtagtag ttgtcgcctt ctttgcgttc
    18961 ctctgcttcg cgctttccac cgacggtgag ccggttgccg gtatgggtga tttccagatc
    19021 cgaaagcttg actcccggca gatccgcttt gaacaaaaag gcgtccttgg tttctttcac
    19081 atcgaagctt ggcgcaaact ccaagctgcg ctgaattgct tcctgcggcg gtccgaaacg
    19141 gagcagtcct tccatcacgc ggaatggatc aaattccgaa aacagcgacg ggaactgggt
    19201 agaaacagga cgacgaacaa cggcggtcat ggctatcctc ccaaaagtat ctgtttgtac
    19261 agagagtcgt tttgcacagc tcccaatgcc ggacatatct gccgcatacg gagccgtaca
    19321 caaggtctgt gtgaaactgc aagggaagcg gcgtgtccgt cgtggtcacg cactccctag
    19381 cggtcactca aacagataag ttttggccaa acgcggatca agggggggtc gaaagatttt
    19441 ttttcagccg cgaaaaaaat ccctgctggg tcttttgcaa aagcgcttgc gcaagcgctt
    19501 cggtagacca gttcgaccag actttcccgt cgatgatcag ccacattccg ggtcgcagcc
    19561 caagccgctg aagctcggcc acgtccgaca tcacctgatt gcgaccacgg gcgctcgccg
    19621 cttccacttt gccaagctcg ccccgtttgc gcagaagctg tacgacatcg tcgtagcgca
    19681 agacaaagcg ccgcgtcatc agatcggcca cgtcttcgag cagacggaaa aacagccgcg
    19741 gatcgacggc agccgtctgg gcgataagct cgcttgtccg tcgcgccgct ggcaaaagtg
    19801 gcgcatgcag cacacacagc cgaactttgt cgcggaagcg cgccaccgca tcaagcaccg
    19861 cccgcgtctg cacgccggtc ggaaacgatt catgatggaa aaaaagcacc agcgtcgtcg
    19921 gtgcgatctg tggaccttgg cacggcacac catcgagatg tacccgcaag ggccgattcg
    19981 gaatgtcccg ctgtggatcg aaaggcccgc tgccccagat cacgtccaag cgtcgccgat
    20041 cgtcatcagg ggtcgtctga agctgttcat acaggcgggt cagcggtgtc ccgtttttca
    20101 gcatgtcgtg cgcccgcagc cgcgccgcgt cgatttcatc catcacccgc aattcgagct
    20161 gcgccccgac cacccggcgg tcgttgacca ggacttcggg catctgccga ttgccgcggg
    20221 cgctcgccca catcgccgca agctgccgct gggtccgatg cgtccgcaac gactcacgca
    20281 gcgacgccgg atcaaagcca tgccgcgcca ccgcccgcca caactcctcg ctcagctccc
    20341 ccggcacggt cggcgtgctc gacacaccga gcgctgtctg ggcaccgggc gaaagccact
    20401 cggggtgttc acacagctcc aacagcagcg ggaagcactg cgcgggttgc tcaccgcagg
    20461 ccgcggtcca cagctcggcg ccacgctcgc cgccactgcg cagcggcaga aaccgcaccc
    20521 ggacatcacc aagcagaggc tgccgacgca ccaagctgcg cagaagtccc gccggacacg
    20581 tcccgccgag tgtcccgctc acatcgaccg tcaccacggc aaagcgcggg cccagctgcg
    20641 gcacaagcgg cggcggcgcc aaccacacct cctgcgcggc gtccccggca agcggcgtcg
    20701 cccagcccgc gcgcaccgac agaacacaca acacagtggc aagcaccagc gacaaaacag
    20761 cgctcgtccc gtcgcacaat gcacccaaac ggcgccctgg ccgaccgatt ctctgttgcg
    20821 cgtagcgtgc caccgtgcct tcgtcctcgc tgcgtccgtt actccattgg tagcctaatc
    20881 atcggctttt gtggtacacc gcaagcctat gcaaagacga gttgtggtca ccggcatcgg
    20941 actgtgtaca ccgcttggac tgggtcgcga ggcgagctgg aaagcttgca tagcggggca
    21001 gagcggcatc ggacccatca cccacttcga ccattcgctg ttcaccaccc acttcggtgg
    21061 tgaggtcaaa gggcttgatc cgacgacgtt catcgacaag cgcgaagcca aacacctgga
    21121 tctgtttgtc attttcgcgc tgtacgccgc cgaagaagcg atggcccacg cgcagctcaa
    21181 ggtcagcccc gagcccgccg acaagcctgg tctttttgtg cctggtgcgc agctgccgtt
    21241 tggggcgcgc agcgacggaa gctatccatg cgatcgcgtc ggcgtgttca ttggcgccgg
    21301 tcttggcggt gtcagccaca tcgaatcaac gcactcgacg ctgaccgagc gcggaccggg
    21361 tcgaatttcg ccatactttg tgccgcagat catcatcaac atggcaccgg gcctcttgtc
    21421 gatccgcaag ggtctaaaag gtccgaacat gagccaagtg tcggcctgcg cgacgggagc
    21481 ccacgccata ggcgaagcgg ctcgcaccat ttctgaaggc gctgccgacg tgatgattgc
    21541 gggtggaacc gaagccaccg tgtcgccgct cggcgtcggt ggtttcaatg ccatgcgagc
    21601 gctgtcgacg cgcaacgatg caccggaaaa agcgtcgcgc ccgtttgatg tcgatcgcga
    21661 cggctttgtc atcgccgaag gcgccggcct gctcgtgctg gaagaacgcg agtcggcgct
    21721 gcgtcgtggt gtgccgattt tggccgagct ggtcggttac ggacgttctg ccgacggcta
    21781 tcacatgaca tcgccgtgtg tcgacgggga aggcgcgcag cgctgcatgc gaatggcgct
    21841 tcgccaagcc caccagcacg gcatttcacc gactgacatt ggctacatca acgcccacgg
    21901 cacatcgacc cggcaaggcg acatcgccga aacggtcgcg atcaaagcgg tatttggtga
    21961 tcacgcccgc aagctcgcgg tgtcgtcgac gaaatccatg accggacacc tgcttggggc
    22021 cgccggtggt gtcgaagccg cgttttcgat cctggcgctg caagacggca tcttgccgcc
    22081 gaccatcaac ctcgataagc ccgaccccga gtgcgatctc gactacatcc cgcacaccgc
    22141 gcgcgcggcc cagagccggg cggcactgtc caacagcttc ggtttcggcg gcaccaacgc
    22201 ctcgctgatc ttcgccaagc acatctgaca taattccttt gtggacaacc gcgattttcg
    22261 ccacgagtgc gttcgccgtg gcgatgtgct catcctgcgt gtgcgcggcg agtttacgct
    22321 cgacgacgcg cgcgcctacg tcgcgattcg cgatcagatt ggcgccgagc agaactatct
    22381 gctgctgctg atatttgcca gcgaagcaac cggcatgcat cccgcggcac gccgttacgt
    22441 cgccgaacac gccacgccgc acgaaggcaa aaacagcgcc attgccgtcg tcggagccgg
    22501 cgcgattctg cggggactcc tacagcttct gttttcggcc atcaccctgc ttggccggcg
    22561 tccggtattc ccaagctttg tcagcgatga agccactgct ctgcgttttc tcgatgagca
    22621 gcgccagcgc atctacaaac agctcggtcg aaagtagccc cgttccccgt cgcttacgca
    22681 tcgccgcgcc gccgccgttt catcttgcta tgtttcatcg aggactcggt ttagattgcc
    22741 ggttcctatg ccttcccagc cacatgaatc tgcccccgca tcacacgttc cagctaccgc
    22801 gtcttcgcac cgcacggcgc ttggaatcgg cgcacttgcc ttggtgatga tctattacgg
    22861 gatctttgtc atacgcaatt tcagcggccc cattggtgac tataacgaat cattcctgca
    22921 cgaatacttg gcctggtacg catcgaagca catctcgctg tggccaattc cgacccttca
    22981 tctgcatacc gatcaagtcc tgtatccgtt cggtgccaac gtcgcgctac agagctggtg
    23041 cgtcgagcgt gacgttctgt ttgcgctgct caaaccgctc tttcccaacg ctccgctgct
    23101 ccagctctac tacttgcttg gtgttggact tagtacaatc ggtagctatc tgctccttct
    23161 gccgcgcttt ggtgcgcttc gatcgggcat cgcgaccgtg cttgcgcatg cgttcaactt
    23221 ttattcgatg cagaagtacg cctatcactt caacatcgcg tgctaccact ggaccacgtt
    23281 aggaattgtc tgtgactttg tgctagtgaa aactgtcgtc gctgatcgcc tgattccgct
    23341 gcgacttctg ctgctgcgat gtttgcttct ggcgctcgcg tttggtcttg aactgggaca
    23401 cgtacttggc tattccctga cttcgtttct gttcgccagt gcctatgtgg tgtggtttgt
    23461 ccgcagcgag cagcgccaag gcagaatcgc cgtgcagcag cttttccaca gtctgcgcga
    23521 tcagcttgga cgcgaccgtc tgtttgcact tttgatgctg tcttcgacgc tggcggttgc
    23581 gtggctgtat ggcgcgatca cgttcggaat cgttcgcgac tcgctgtcgg tgggtaaatt
    23641 tccgggccaa gcagcagcct ggtgggcaag tcccttgcgt ctgctgattc cctatacgcc
    23701 gtggcttcat ccatccatgc agccgctgtt taaccacgtc ctgggcgacc ttgcggaaga
    23761 cggaatcggc tgcggcagtc cggggctgtt tctgctcttt ctggccggac ttggcatcta
    23821 ccaaaccaaa gcgtggcaaa gacgatcgcc gtacttcttt ttgttcgcat cgttttgtct
    23881 gtatgtcctc agcggcgcac atctgccgct cctcaagctc cttccgtggt tcgcattctg
    23941 tcgggtctat tcccgatcca cgctggtcta cagcacgctg ttggcgcttt tcgcactgga
    24001 agtttccttt tcggttgtgc gcacgtcgat ccgccgcagt ctgctcctgc ttggaatctt
    24061 gggaatcggc ggactcgaac ttgtgaccgt cgcccagatc aagcgagagc tgccagcgta
    24121 ctcattttct gcgcagcaaa aggactacct tgcgaccatc aaaaaacttc cgggtgaagc
    24181 gctgctcgac ttccctttct gtctcaccgg aggcaacggt gacatagccg gactctgtcc
    24241 gctgtggcag cggttaaaag gggtctacgc actccagcgc tttcacgaaa agaaagtgat
    24301 cggacagtac ttgggaagac tgcatcccaa ccagaccgcg ccgtttgtgc gacaaggatg
    24361 ggatacccta aacgatccgg atacgctgcg catgaccatc gcgcaaagac agcgccgctg
    24421 cctgacttcc gacgagtggt tcttcttcga gcagttctat ctgtacaacg actttgccgg
    24481 gattcagctt gcgatagaca ggctgcctcc gggctgtccg gaacagtttt acaagcgcta
    24541 cggagccccg attgcaagca tggagctggc cggagcagga cgcctggtgc tgattcccaa
    24601 gccgctctcg cagcacgcgc tcgtgaacaa agagatgggc aaacaagtca cgctgacgcg
    24661 caccgaagcc gcgctgttca agcggaagct cgtggctgcg gatcttcagg tgagcagtcc
    24721 cacggatgtt cacgagatca caggcagcga aggacagcca cagtcctatg attttgtcga
    24781 cggtgacggc atcgtgtacg gaccctatgt ggcgcttccc aaaggacgct atgtggcgac
    24841 gtttgaactg gaggttccgc agccgggcac aggcacactt gccaagctgg atgtcgccgc
    24901 acacaacacg tcatttgcag ccaccaaagt gcaagccgat tcgttgcctt ccggacgaca
    24961 aacagtcgca cttccgtttg aactttcgga acggacttca gggatagaaa cgcgcgtgtg
    25021 tgcaaagggc acaagattca ttctgcacag catcaccctc gaacagcttg gcacacagaa
    25081 caactaactg gaaaaggacg tgtcccatgc aagcgctgca ccgtttcgga actgcactgt
    25141 ctgttcggct ctccgccgca ttcctttttg gaagtctgct tgcagtgacc gcctgttccc
    25201 ccgcacgggt cggcgtcgaa attgatgaca tgggggtgcc ccaggatctg acggtgcgcc
    25261 cacccagacc ggaccttgcg ccgccgccgg tttatgattt cgcgctgccc gatgttcatg
    25321 tggtgatcac cgcagacaac gcctatgcat tcggctttgg ctttgctgat cgcgtcacca
    25381 agctgaacgg acgaccgaaa actgtcgtcg ctggcgacat ttttaactgt ccgatagggg
    25441 tcggtccgga agcctacgac atccctgccg ctgaagcccc agagtcttcc tatctttaca
    25501 tcgtggcctg ggccgatgat gccgtcaccg aaggtgtact cggccagttc gagcgcagcg
    25561 gaacaccgct ttacaccggc gatgcaaact ggcaagtctg cgccacaggt cagttttacg
    25621 atcccggacc agccggtggt ggacccacgt tggacgttgt aaaccaagag cttgcgcgct
    25681 gtaacacagc agccggttca ccggcgacca gcagcgccgg ttgggtatcg accacagggg
    25741 ccgtcacacc gggcgcaatc ggcaacttgg cggtcggcga agacaacagc tccccagatg
    25801 gtgacttccc gatcgtctgc caaaaagacg cgatgggaca gcgtggcatt gattccggag
    25861 cccgctggat gtggtacagc cccgacggaa aatcgccatt ccgttacaca ggtggaaaaa
    25921 atccaacccg gaccttcctg atcttccgtc tgcccagtcg cgtcgtggtc atcgggtaag
    25981 cgcgcccggc actacttgcg cagcgacacc atcgtcacac cgcacagaac cagcacactg
    26041 ccgataagct ggcccatcgt cacctgttca cccagcagga aatgggcaag cagaatcgtt
    26101 gacacggggc caatcgtggt caatatcgcg gcgggtccgg aacccaccag accgattcct
    26161 ttggccaaca gccaggtcgg aatcaccgtc gctacggtcg ccaaaagcag cccgtatcca
    26221 tacaccggca gcggcaggcc cgaaagcggt cgaccggcca caatccagtg cacaaaaatc
    26281 gcgaccgtgg ccgacagaag tgaataggaa ttgaagcgtg ccgcacccac tttgggaatc
    26341 agccgacccg cgcccagaag atacagcgca tagccaaccg aacatcccag cactagcagc
    26401 gagcccttca tcacgagcgg accggacaga ttttgttcgt gatgaaacac aattcccatg
    26461 cccgcatacg tgaccccaag cgatagtaca tgcaggcggc gcagcggcgt tcggaagagg
    26521 agtgccgcca agagaaccac aaaggtcgga tacagaaaca gaaccagtct ttcgagggag
    26581 gcagagatat acaaaagccc cagaaaatca agcatgctag atatgtaata tccagtaatt
    26641 cccagcagca cagtcagcgc catttcacgt ttgctaagcc gcgcggtcgc ttttttttcc
    26701 gaaaaatacg cggtcagcag aagcagcggc acggccatga acatccgcaa ggacaatacc
    26761 tgaagcgcat ccgcgccgag tcgataggcc agcttggcca ggatcgcttt ggttgaaatg
    26821 gtgatgccgc tgagcagcac caacatcgca ccttcgatcc gctgtttggt caggttccca
    26881 tctgacatga tcgcttccca agcgaacccc ttatgggggc gaatcgccgt cgctgccttc
    26941 atcactccga tcgccagaaa gatgcgccac gacaagtcgc agtccggcat ccattgattc
    27001 ctgattgcgg agcttgccga actgttcgtc ccagcgacca tgcttcagat cggcgcgaag
    27061 ttgggcgatt ccctgcacca gctccaccgg attgagaagc tgaaacgccg acatcgaatt
    27121 tcgtacactg ggatcaagat acacttccgg tcggccccaa aatgcatcaa tgaaaccgtc
    27181 ggtgcagtcg tgcggaatca agacagggat gctcgtcacc ggtcccagtg cttcttcaaa
    27241 aaaactgcgc gctggcatcc ggcgacgatg cgcggcagcg attccgggaa agtacacatt
    27301 ggtgatccag cgatcgggaa acacctcacc gtgcggaaga taagtcagga tcacgatgcg
    27361 ctggcgggcc acacgacgca gctcacgaag cccacgcgga acatccagcc agtgatgcac
    27421 ggtcataaag gccgtcacgg catccaccga ctgatcgatg agtggcaacc gttccgcttc
    27481 gccaacgatc gctggcgccg catcaagtgg acgctgccgg atcatcattt cagatggttc
    27541 cagcgcgacc actttgcgat tcggcgattc ataggaaccg gttccggccc cgacgttgag
    27601 caccgaaaca cagtctccca gcgcctcttc gatgagagcc gcgaaccgtg ggtcgggacg
    27661 ccgcttccgc tgataatctc tgccaatccg atcgtaccga gccactcttt ttcgcttccg
    27721 ttacaaagga cgaggcggaa gttcgtagca ttctggcaaa cgatcgactc cgcaccgcag
    27781 cgtctcacac cagttcaaaa actgtgtcac catttcgcga ccgcgaaaca gggccccatg
    27841 ttgcggcgca atgacttcaa tgtccagcgt tcggacaagc ttgacccacg ttttcagcgc
    27901 caggctggac gccatgtagc gcttgtgaaa tccgctcatg aaaggtacgt ggtcttcaaa
    27961 gtccggaact tcacggtatt cgcagcccag cgacgcacca acatccccgg tgtagagaat
    28021 tttggacgtc ggatcataca agtgaaagtt tcccgccgag tgcaggaagt gagccgggat
    28081 cagcgccaat tccgatccat tgagatcgag ccacattcct tcatccggaa tcgcgcgcag
    28141 ccgatgttca aactgctcct cgatctcgac atgcggaatg aagcgtagcc agagcagcga
    28201 cacgtatgcg agcgcatcgg ttgcccgcag ccaatcgttg attcctgaca ctatgtcagg
    28261 atcttgatgg gacaaaaaca ggtggcgaat ctgtgcacca cccgacatca cagcggtgtc
    28321 ctcaaacaac cgctggaaca ccgcgtgtcc cccaggatcg aggagaatgc actcgccgtt
    28381 gtgaacgatc aggtggtggt tcgcctgcac cgcgaggccg gaagtaccga ggttctggag
    28441 aagaatgttg ctatgactct cgccgcgaaa cagggtgatc tcggacatcg tggtcttgct
    28501 cctgtcagat tctgcttacc agcgactgcg ttagggggcc cggtcgtgtc gaatcgtgcc
    28561 ggatgaaaac agccgcgagc gccgcgcgag gaatcgctcc acagcctgtg tcagcagctt
    28621 gggatcgcgc gatttcggaa tggaaccatc agcgccacag cgacgggcaa gctcggacag
    28681 ctccgcctct ggaagcgagg aatgaagcag aatcacaacg cctttcccat agctggcggc
    28741 acgcacaagc tgacagactt tgtcacccgt aatggacggc atgttcacgt cgacgataac
    28801 catatcgggc tggtgacgaa tgacctcacg ggtgatacca agtggagagt cgaagctatg
    28861 gactacatac ccctccgctt gcaagagaag ctgaacgtag gtaagaaatg tttgactgtc
    28921 gtcgagcaca aagattttgg ctcgctgatc atgttgcgga gcgcgctcca tgggaatcgt
    28981 cctctggcga ggtgttcact cggtgtaggc ccgcatcata gcaggcaccc gcttttactg
    29041 tcgaacgtaa tattggatga cagtcccact ttgtcaaatc acaacacatg acaaaagtcg
    29101 caaacgacat ccacagacat caatatatgt agcattctat aagcttcata ttgagtagta
    29161 tgtttccgca ttttccgtcg gttcggaatc gcacggagac agcattggct cagcatccga
    29221 ccattgcgcc gcacaagcaa aacgcatctc cgatcgatgc atatcgacgc gatccccagt
    29281 cagaagatgt ccgggctttt ccggtgacga cagtattaca aaaaatagtc cgtctgcgtt
    29341 tagctaatga tttttgttcc gcaagtacgg tcgggtccat gtaaaaatgg atcatcgata
    29401 cagccggggg gctgcttcgc gatgggggaa gccagcaaag tgtcaggggt cacgcaagag
    29461 ctgctcattt tcgagcttgg ggaacggctc tatgcactcc cttcgagcca agtcagagag
    29521 gtgctgcggg ccttcagcgc ggtgccgatt cccaatgcgc ccgcgattgt cgaaggactc
    29581 ctgaatgtcc ggggtgacat cattccgctg ctcagtctgc gtcgccgttt gggaatcgcg
    29641 gagcgtcccc tgtctttgac cgaccacttc atcatcgcca aagagggaag tcggctgctg
    29701 gccctgcgtg tcgatcgtgt cgtcaatctg cagcgcgcca gcttccaaag gcctttcgat
    29761 ccgggccgct atgcgtcggg aagcgaagtc atctctggcg tcgccacgat tccggatgga
    29821 atggttctga ttcacgatct gggacgattc ctatccgctg agcagagtga acagctcgac
    29881 cgcgcagtcc gtgatgcagc gggagccgtc cgcgaagaaa aggaatcgac atgagccagg
    29941 aaccaaagag ccacttccag cagctcattt ctcggctggc caaggtcgcg gcggcgctgg
    30001 atgaatcgtt ggcacacacc gaagcccgtg tccgcgagca gcttgatgcc cgcgccagac
    30061 tgatggcgta tgccgtcgac cgcgtgcgca gtgaagacca ggggatggaa gtgctggcct
    30121 ttttgctcgg ctcgcagcgc tgcgccatcg cgactcgatt tgtcatcgag atgtttcccg
    30181 ctccagccat cacccgcttg ccaggaaccg acccatacat tttgggaatc tgcaacgtgc
    30241 ggggacagct gctcttggta ttcgatctgg ctcttctttt cggtttccct tcacaaaaga
    30301 cagacagcac catgcagatt ctgttgcttg gtgcagaacg tccggaattc ggtgttctct
    30361 gttgtggcga tggactgacg ctgcggcttc ccaagtcgag tctgtcacgc tctgccgaac
    30421 tgcatgagcc tccgtttttc ggctgtattg atgcagtcac cagcgacgga ctcctcctat
    30481 tcgatggaaa agagctgatg cgagatcagc ggctctttgt caaccagacg ggatgaaaga
    30541 ccccatgagt actttccgat ctgttctttc cagtacacag aagcagacca gctatctgct
    30601 tctgctgatt gtgtttggtg gacccgcgtt catcatcgtt cggctcctgt ttcctgatct
    30661 ggcgggacca agtcccaatc caaatgagcc agcctttgtc ctgacctatg gaccaccgct
    30721 tttcgccgca cttgctgcga tggggacgct cttacagctc cagcgctact tcaatcagtt
    30781 tcatcgattc acgacgctgg gtcagcgaat gccaagcgcg ctcccacaag cggattcgca
    30841 aggtcgcagc atcggcagcg aaacgcgaga gctcatcgac acgctgattt cactgcactc
    30901 cgtttcgcaa gcgctgcgca gcgaaaacga gcagcagcgc ggagaacttc agcggctcag
    30961 cagtcactat gaatcgcttc atcagtctct gctttcacgc atggaatcgc tgctaagcgc
    31021 cccggtggaa gaactgccag atcgagccag cgcccgccaa aacgcgccgc atgcgatgga
    31081 agggctgcgc caaaatctgg atgacgccct gacccgtgcc cgatcgcagc gtgacttgat
    31141 cgatcagatc gtcagacaga cgcatcacag ccatgcagaa acagagtccg cgcacgaacg
    31201 tcttttgtct ctgcggagca aaagcagcgc gctgtctaca gccatgcagg aactgatgaa
    31261 tcgcacgcag accgtcggac agattgctgc atcggtgaaa cttctggcca gtgaaattga
    31321 tcacgtcgca ctcaacgcaa cgattgaagc cgcgcgcgct ggtgatgtcg gtcgcggctt
    31381 ttccattgtg gctgcggaag tccgttccct ggcagaacag tccaagggtg cggcacaaaa
    31441 aatgcgccag gtgctagagg aaattcagca agccagcaaa agcacgctgt cgttcgcgat
    31501 ggaaagtgaa cagagcatcg aagagctggc cgataccacg cggcagactg cggcgtcctt
    31561 gctgtcgcta gaaaacgaag caacggaagc gcaggattcg gtgtcgcagc ttctgtccag
    31621 catggaatca cagaccggca gtgtgtccgt gatccaagcc gccctgcatg gaactgcgca
    31681 gggaagcgag cagctccagc gtcttatcca tcgcaatgaa gagctgcggc acattctgta
    31741 ttcggcccag accgaaaaaa cgatcacggc gagcgcggta tcccaaggag ccagacgatg
    31801 agcagcgatt cctttttatc gcggcgacgt ttcgggacgt tgcttctgtc atccacagca
    31861 tcgctcctgg ctggctgtcc gcagcgcagc ggatcgtcag atgatgcagt gaaagtgggg
    31921 gttctgcacg cgctcagcgg aacgatggcg atcattgcca agccggtcgc agaaatgacg
    31981 atgtttgcga ttgaggagat caatgcgcaa ggcggtctgc tgggaagaaa acttgtcccc
    32041 cttctggtcg atggccgatc cgatcccgcg gtgtttgccc gtgaagcaga gcgtctgatc
    32101 gtcgacgaac atgtcaaagt tgtgttcgga ctcctgaact ctgcgagtcg gcgcaccacc
    32161 aagtcggtct tcgaaaagta caaccacttg ctgttctatc cgatgtccta tgaagggctg
    32221 gaacagtccc ccaacatcat ttacaccggg gccgcgccga atcagcagat cacacccgcg
    32281 gtgaagtggt gttttgacaa cctgggcaag cgtttctttt tggtaggaac cgatcaagtt
    32341 cattcgcgcg ccgccaacgc catgatccaa gatcaggtct ctgctatcga cggtgaagtg
    32401 gtcggtgaag cgtatgtttt gtggggcagc aaagacgtaa aatcggtgat cgaacagatc
    32461 gagacccgca agccatcggt catcgtaaac agcatcaccg gggattcaaa cctggcgttt
    32521 ttccgcgagc tgcgcagagc cggaatcacc ccaaccaaaa ttcctacgct gtcgtttgcg
    32581 ctgacagaac atgagctggc cgcgctgaac acgcgcgaga tggcgggcga ctatgttgcg
    32641 tggaactacc tccaggcgca gtcgaaccct gaaaacatcg agtttgtgaa acgagtcagg
    32701 gctcgcttcg gggccaactc cgtcgtcgat gatccgatgg aatcgggcta cctgagcgtg
    32761 tatttgtggg cggctgcggt gcgcgcggcg cgttcctttt cccctgatct ggtgctggga
    32821 acgcttccca accaaagcta caacgccccc gaaggtctgg tgtacgtcga cgccgaaaac
    32881 aaccatgtct ggcgatcggc gcggattggc aaaatcgata ccgatggatc gtttgatctg
    32941 gtgtgggaat ccggacgtgc gctgcgtccg gttccctatc caatctaccg aacgaaagct
    33001 gactgggaga cgctcctaaa cgacctgttc attggttgga agcgtcagtg gatgaaccga
    33061 ggttaagagg cttccatgca gattcctaca acacgagagc tgcttcgcat tctaagcaaa
    33121 ccacgtctgg caacccgact gcttggctgg tttttgattc tgaccacggg accgcttgcg
    33181 ctcatcatgt acatcacgta tgagcgcggc gcctcggcga tccggcgcga ggtggaacag
    33241 tcgctggaag cgacatccaa agcccgcgcg gtggcgatcg aaaactacgt tgtggagcgc
    33301 aaacgcaacg cctcgggtct gtcaagaata cctgacatcg tggcaaccat ggctgacttt
    33361 caccaagcgt tcacccgcgg cggcctcaag tcaccggcgt ttgatgaaat cgatcagcgg
    33421 atgcgcccgt ttcttacttc ggtaaaggag tcgcgcggct attccgatct gttcctgatt
    33481 tcacccgatg gagactgtct gttttccgtc gccaaagccg aagtctgggg cacaaacgta
    33541 ctctccggac cgtaccgtga ttccagcttg gcgcgggtgt tcgaacgcac gcgatcgatg
    33601 ctcgattccg aagtgtctga ctttgactac taccaagcga gcaatgactc cgccgcgttc
    33661 attgccgcgc cgatcatgcg tggcaaaacg ctggtcggaa tcatcgagct tcagctaaac
    33721 aaccaagaaa tttatcgctt gcttgcggac tacacaggtc ttggcgaaac gggtgaaacg
    33781 gtcgtggtct cactgcgcaa cgacaagatc atttacatgg caccgacgcg ccgcgatccg
    33841 gacgccgcat tccgccgcca aatctggaat cgtgatcttc ccaaataccg cgcgctttat
    33901 gaatcgtcgc agggtcgcac gtccacgtct gcggaaacgg tcacagacta cggcggcaag
    33961 caggtcttgg cggtatggcg atttttgcca tcgttccgct ggggcatggt gaccaaagtc
    34021 gacatcgagg aagcgaccgc agccatcacc gcgatgcgcc gcgatcagct tgtgatcgcg
    34081 ctgtttgcgg ttctggtggt gattggcgcg gcgctgtacg ttgcgcgatc gatctccgaa
    34141 ccgattgtga aactcaccca gagcaccaat ctcatcgcca gtgggaatct ggatgaacgg
    34201 atcgatatct cgtcggaaga tgaggtcgga gagctggccg cggcatttaa caaaatgact
    34261 gcggacctga aaaatacgct ggtttcacgc gatgcactca aagatcagat tcgccaccgt
    34321 gagctgctca ctgcgacgat tcgcaatgtc gtttcgcaac tgggaaccac cagcgcggac
    34381 attctgaatg cagcagccaa gctgtctcat ggcgcacgcg agcaagccag tgcggtcaaa
    34441 gatgcagtca ccgcagtcga tgacgtgtca cagactgccg cccaagccgc aagccgcgct
    34501 cgcaaagttg ccgactctgt tcgccgttcc gaagaagtcg gcaaggccgg acgcaaagca
    34561 gtcacagaca cggtggaagt gctcggcatc gcaaaggaac gatcagacaa ggtggcagaa
    34621 aacatcgttg cacttgctga aaagacccaa gccataggtg agatcattgc caccgtaagt
    34681 gagatcgcag aacagaccaa cttgctgtcg ctgaacgccg caatcgaggc gtcgcgcgcg
    34741 ggggaacatg gacgcggctt cacggtggtt gcaagcgaag tcaaagcgct cgcagaacag
    34801 tccaagaaag cgaccggaca agttcgcaac atcctagaga gcattcaaaa agcgatcagt
    34861 acggcggtgt attcgacaga acaaggcatc cacagcatgg tcacagcaac ggaagcggca
    34921 ggccaagccg gcgaaacgat ctcacgacta tcagagatca ttgccgaggc cgcaacggtg
    34981 tctacgcaga tcatgaactc ggcgactcaa caggcgacag gaatggatca cgtcggttcg
    35041 gcgatgggaa gcatcaatca gatcgcgacc caaaacatgt ctgccaccgc gcagacagaa
    35101 cacgcagttc gcgagctgaa cggaatcggt ctcaagctca ccgagctgct gaaggattcg
    35161 ggacaagctg gagcgtaaga catggacaag tctgtcctca tccagcgtct gcttgagaca
    35221 tttctcgtcg agttcgacga gcgcctgcag gccatgagcc gcggtctgct ggcgcttgaa
    35281 gcgagtgcga ctgggactcc tgagcgggac aaaaacctgc aagaacttct gcgcaatgcc
    35341 cacagcctaa aaggagctgc gcgcgcggtc gaagtatccc tgattgaaaa gtcctgccat
    35401 catctggaag agattttggc ggcggtgcgc aaccatccga ccctgctcga ccaacacctg
    35461 attcagcttc tgtttggtgt gctggacgga ctggcggaag caggaaagct gcttcggaaa
    35521 caggaaccac tagagggctc gctgttgttt ggcgtagcgc tcgaaatgga gatgtttatc
    35581 gagagcacca tccgtccgct tggaaaggaa ccggcccagg ctctagctgc gccgccacca
    35641 gcacagatcg cgaagtccga tccaaacgct gatgccgaaa atgcggtctc ttccgatctt
    35701 cctacggttc gtatccacac cgaaaagctc gatgcgctgc tcacgcggag tggcgagctg
    35761 ttgactgcgc gacaacatct gcgtttgaca agcgagcagc tttcacaggt cgcagattcg
    35821 atccgaagac tggaatcgga gtggcgccac cacggcaagg ggaatgccaa aatcggcagt
    35881 cccaccagca agacagcgac gaaaacaggc gattccctgg tggaagagac gcccgcacgg
    35941 ctgaccgaac ttgtcgccca gatcgacaag cttcagcttg cattgcgcca ggacttgcga
    36001 cagctggagc acactggatc agcgctcgat cagcaggtgt cgcgcattcg catgctgccg
    36061 ttttcgcatg cgtgtgtggg ttttgatcga atggtccgcg atctggcccg caacagcggc
    36121 aaagacatcg agctacgaat cgaaggcggc gatgtcgagc tggatcgctc cgtgctcgac
    36181 gcgctcaaag atccgctccg ccacctgatt cgcaactcga tcgatcacgg aatcgaaaga
    36241 ccggaagacc gcaagcgcag caaaaaatcg ccccgtggaa cgatcatcgc ttcagctacg
    36301 ttgcgcggtg gacaagtggc cattgtgatt tctgatgatg gtcaggggct tgttgcggat
    36361 tcgattcgca agcgtgcaca gaagttggga atgccgattc ctgacaccga tcagaagctg
    36421 atcgatgcga tctttatgcc gggtttttcg acggcagcgg actctgtttc gatggtctct
    36481 ggacgcggca ttggacttga tgtggtgcgc aatcagatca agtctctgca tggcgaagta
    36541 gacgtttcgt ttgtgcccgg aaaaggaacc cagttccaac tgactgtgcc actgacactg
    36601 acgcttctgc gggtacttct catccgtgcg gcggatcaga cgtttgtggt cagcaacaac
    36661 tacgtgcaca agctgctgcg gctccggcaa aatgaagtga tctggattgg cgaaaggccg
    36721 atgattgtca tcgacaatca gcccattcat ctggattcct ttgcgcatgc gctgtcgctt
    36781 cggcaaccga caggaatcac ctggcaggga aagcgtccgg tggtggtgct gcgttccgaa
    36841 cagcgcaccg ctgcattttt ggttgatgag cttgtcgccg aacaggaagt gacggtcaaa
    36901 agcctgggaa gtcgtgtaaa gcgaatccgc aacgtggctg gcgcagtgat catgcccgat
    36961 ggccaggtgg cgctgacgat tcacgttgcc gatctgttgc acaccgagaa gccagatgaa
    37021 tcgctgggcg cgcttcgaca tggcggagca caagactcgg gtgagcgcaa acagagacgg
    37081 atcttggtcg tcgatgactc ggtgacaacc cgcagtctgg aacgaagtct gctggaatcc
    37141 gcgggctttt cggtcgagct ggcagcagac ggagtccaag cgtgggatcg cattcagtcc
    37201 ggtgaaatcg atctggtgct gtcggatgtc gacatgcctc tgctcaacgg ctatcagctc
    37261 acgcagacaa ttcgcaagtc tgagctcttc cgtaaacttc cggtcatctt gatcaccagc
    37321 aacgatgatc agcgcagccg cgaacgcgga atcgaagccg gcgcgagtgc ctacatgacc
    37381 aaaggcacgt ttgatcagag cgtcctgctc agcgtcatcg aacagctcat ctagtctctt
    37441 tgggaaaaac gccgcgtggc gcacaccgat tacgccctac acgatgcgtt tttccgtcga
    37501 aaaacgggct gattcgcgcc tgtcagggga ctgtttgtat aatgtctagc aagctgtccc
    37561 atttgacata aaaaggaatg tgtatgggtg tcgtgatcgg ctctgtcacg gaagaagttc
    37621 caggacttgc gatacgcagc ttcctcgatc cggaaggact ccgtctttcc gaagaagatc
    37681 gcgagccgcg cagtacagag gcctggattc actcgatcgt actacacacc accaaaggca
    37741 ttccgggtgg ttccgatcag cgaagacagc aaatcctcga tggaagtggg ccgcacaaaa
    37801 accacatcgc tgcggttgca caggcctggg catcggatca catctgcgcc agcgcgcact
    37861 tcctaatcga tcaggatgga accattgcct gcgtcgctga cttgctgacc gaaacgacgt
    37921 accatgccgg gcagcgcgcg gtgaatcacc actcgatcgg aatcgaaatg taccaaggtg
    37981 aggaagcaga gctgtacgct ttccaactgg aacagactgt ccgtttggtg gattttttga
    38041 cccggcgatt cggcattcag cgacagtttc acgcccccta tcgcaaccaa gcggtgctgc
    38101 ggataaagga aggtggttcg tcggtggttg ccgtgtatgg acaccgtgaa gccagcgatg
    38161 atcggggggt cggcgatcca ggcgatgcga tcttttcggc gctccttgcg gcgggttatg
    38221 aatgctttga cttcgacaag gaagaagatc gcgcggtgtg gaaagttcgc caaacagacc
    38281 taaaccaaaa gctcggtctg tcactgactg tcgacggagt tcccggtccg gaaacggtcg
    38341 tggcactgcg aacggcgggc tacccgcatg ggatgtggat tccgcgccca ggggactgat
    38401 tcttccaccc cgcacctgat gtctgcaccc cgcagactgc aagcatcacg cgatcttttt
    38461 cgacggaaac acgctggcac agccgatgca acagcggtcg tttcatgatg aaacaaaccg
    38521 cacgcattgt cctttccggt ctgtggctcc tatcgggttg tggaaccggg gaagaaatcg
    38581 aagagatcga cgtcgcgccg ctggaagaaa aggaaatcac ggcgacctgt ccgcacaaag
    38641 cgcagctcaa gtggggtctg catcccgacg catcagatcg actgcgctgc atcggaattg
    38701 ccgctgggga catctcgcag acgattggga gtgcgcccgc ttccgccgga tatcatgcac
    38761 aagacggaac cgcgggaggc cagccgtaca ccgcagcggt tgacattcgg atcggcggac
    38821 gcagcgaaag ccagattcgg gcgctgctcg catcgctcgg gtcgtatggc ttcgccgcgt
    38881 ggtatcgcaa gcccggtcac gatggctggc caagcgatga agcaccgcac attcacgcgg
    38941 tctacgccgg ctgcgcgatg aaggcggcgc tcgacgaaca agtcggtgac tatcgcgcgg
    39001 gtcgcaacgg cctgtcgagc cacacccgct acacgttctg gaccgcgagc caagccgtcc
    39061 gagacgccgt ctacaacctg tggaacaagt acaactagcc caggtccgca agaaagccgc
    39121 agtttgccga cataaactcg ccctaccgcc gttcactgcg taccaaatgg tacggtcgaa
    39181 cacctgctta tttcacaaat aatccaaatc ttccgattag gcacaattgc ctattcgcca
    39241 cgtaaactat tattcacagc gtaccgaaat gcacaaatta aacaggatca ttttcatcag
    39301 agcacatcat gtaattgtat aatattacag gaaatttact ggatcagccc tcatgtcaca
    39361 cggctgtcac attcgacaca tcaggtgttc acccgacgga agagccctct attttcgccc
    39421 actatcgcga tatactccta ttgggtttgc gtccaaccca ctgtgcacat cggacgtata
    39481 cacttaggag gtcccaatga gtttgcgttg tctttcaccc ttgttattgg ctttgtttct
    39541 cttcgcgggc tgtagctcag ataccaccaa cgcccaagac atgtcaccgg ccccaacctg
    39601 cggtgccgtc ggccaggcct gctgcgcgcc cggcaacacc tgcgcatcgg gcggaaactg
    39661 cgtcggtggt tactgtcagt cctgtggtgg cgccggccag gcctgctgcg ccggtcgcgc
    39721 ttgtgacgga aacgatgtct gcaccggcaa cggccaaggc atctgtagcc cgtgtggcaa
    39781 tcctggccag ccctgctgcg tcggcaacgg ctgcggcagc gtcgtcgctg gcaacgtcgg
    39841 ctgctgcgtc aacaacgtct gctcgtcggc gggctcgacc tgtccgaacc tgaccggtac
    39901 ttgcacaaat ggaagctgcg gcacctgcgg cggcgccggc cagccttgct gcaccaccac
    39961 cgttgctggc aacaccacca acttctgcac cgccaatggc atgacgtgta gcgccggtca
    40021 gtgcgtcgcc tgtggtggcg ccggtcagcc ttgctgcgcg ggcagcacct gcacaggatc
    40081 gggcctggcc tgtggtggat cgaacatctg tcagcagtgc ggtggcgccg gtcagccctg
    40141 ctgcgctggc aacgtctgca ccgattcgac gacggcgtgt ggcaatggtc tgtgtcgcgc
    40201 ctgcggtggt cccggccagc cttgctgcgc aggttcgacg tgctcggcgg gtggctgctg
    40261 cgatccggga tcgaacagct gcctcgcaag cggatcggtc tgttcaacgg gcgcaacctg
    40321 cagtgctggc acttgcccag tctgcggcgc ccccggccaa gcctgctgcc ccggcaacac
    40381 ctgcggcggc ggcggctgct gcgtcgcggg ccagtgcgtt gcttcgggac agaacattcc
    40441 caacatgacc ggttcggtct gcgcaaacgc ggcgacggtc gcatgcggcg gcaacagcca
    40501 gccatgctgc gcggggaata cctgctcgac ttcgggagcg tgttgcggta gcagcacgac
    40561 ctgcctggct ccaacggcga cttgtggtgg cacgcttggc acctgcaccg ctggttcgtg
    40621 tggcactggc ggcaacgcct gcggcggcgc gggtcagacc tgctgtcaga acgcgacctc
    40681 tggcgcacgc ttctgcacca ccacgggcct ttcgtgcagc ggtggcaagt gcgccgcgtg
    40741 cggcggccca ggccagccgt gctgcgatgg caacagctgc gcaaacggtg gctgctgcga
    40801 caacaacaac cgcacctgca tcgccagcgg tgcgacctgc cccggtacgc tcggcatgtg
    40861 cacaaacagc ggctgccagt cgggcacttg cggcaagatc ggccaggcct gctgcggcag
    40921 cggcgtcgag tgcaccgcgg cgtttagcac ctgcaacaac aaccagtgcg tcgcatgcgg
    40981 tggccagggc cagcgctgct gcacgtccag cagcggaacc gactactgcg gcgctccgtt
    41041 tgcgtgcaac aagaacacga accgctgcgg ctaagcccga atctggctga cagactcgcc
    41101 atgttgtcgg cctgacccca ccctgtgcaa agcccgtcca tccgacgggc catccagtcc
    41161 ctaatacacc cgcagcgctt tggcaggatt gccagcgacg atggtgcgcg gcggaactga
    41221 ctgggtgacg acggctccag cgccgacctg cgcgtttttt ccgatcgtaa tgcccggcaa
    41281 gaggatcgcg ccgacgccga tgtcgcagcc gtcttcgagg gtgactttgg caaacgcaag
    41341 cgggcggtgc aaaatcggcg cagtcgagcc gtcgggcgtc gcgggaagct cgtgggcgct
    41401 cgtcagaatc atgacgcgcg ggccgatgcc gacgtcgcag ccaatctcga tgccgccggc
    41461 ggaatgaaag aatacgtctt ggccaatcca ggtgccgcag ccgacgcgca gctcgttttt
    41521 gtaatagccc ttcaagatgg cgcggtggcc gacgtagacg cactcaccca gcgagacgtt
    41581 ttccggatga aaaatgagca cgcctggctc aatcaccacc gattcggggc aagcggcaag
    41641 ctggctgcgc gaaaattccc cgctgccgtg gctgcgcggc gcaggtcgca ttagtactgg
    41701 aagcgcacgc caaactgggc gcggaacggg gtctgccgac tgagcacggt gccgaacgtc
    41761 gtcgcgatgt cacggttttc aaagccggca tggttgctgc caccaatcgg cgtcgaggta
    41821 tcgagcacgt tgaacaagtc gacgatcagc gacaggtgct ggcggacgag ttcgtacatg
    41881 tcaaactgca tgcgcacgtt gatttcgacg aggtccgggt tgcgcagctc ggtccacttg
    41941 cgcacgtcgt tcgggtccgc cgccggatcg agtccccgcc agccatagcg gtttaggtag
    42001 cccgcgctcg tcggtttgct gccgcgataa atgcgcgacg ccggtgcgcc ggacagatag
    42061 gtcaggttga cgccgccggt gagcccgtgg aacgtgtagc tggactgaag cttgacctgg
    42121 tggcggtggt cgtcggacag atagccatag aaccgcaggt cgcgcggaat gtcgttgcgc
    42181 agctgcgaca tctgatcgtc gaccgttccg tcgagaaacg acagcgtata cgaagcaaac
    42241 accatccagt tgggactggg gttgcccgat accacgaagt cgacgccgtt atagcgacgg
    42301 tatgcctcgg cggtgggacg atagccatag acgttgccgc ggcgaggatc ggcaaactgg
    42361 acaaacttgc taccgtcgag cgcgctgacc gcgtttagct cgatgttttc ccacaggtac
    42421 gacgtcatcc ggtacgagta ggtgatgccg ccgatgatgt tctggaacag ctcgcgctca
    42481 aacgataccg tcaccgaatc ggcgtgcggc ggcgtcaagc tgagcttggc gttgccacac
    42541 tcggtggtga agctgccatc ggcacagcgt ccgcgcagat cgtagccatc gccgccaccc
    42601 gacgacgtga cccagcggtc aaagcgaccc gtcgcgcggt tgaagttcca ggtgttcgac
    42661 tgatacgtcg cgtccgccga aaacgtcggc agaaggctca cgacttcgtt ggcccggcca
    42721 tacgccgcct tcagcagcgt tttgccgtcg cgggtcaggt cgatgttgac accgagccgc
    42781 ggcccaaagc caagcaggtt ctgcaccgtt tccgcgcgtg agttctgcgt cgttccatag
    42841 tcgacgcgca agcccggcac caccgtcagc cacgacgttg gcttccaggt atcttgcacg
    42901 aaaaagccga cgccaaggcc atagcgaacc tgcgcgagcg cgggatcaaa cgagttgaac
    42961 tggtagcacg gcgtcgccgc agaaccggct cggacctgcg gctgcgcctg cacacagccc
    43021 gccgatcggt ccgtcgacgt cggatcacgc gccagcaccg atccatctgc tgacgtgtcg
    43081 tcgacgtaca ccgagttggc cggcgtcccg gtatacaggt aatggcgaat gaactggcct
    43141 tggataccgg ccttgaaggt gtgctgaccg atccagcctt ttttcaccca agtgatgttc
    43201 ggatcgaact gcacgcgcca gcgcttgtcg tcggtaaatc gcgcttcgcc agcgttgttc
    43261 caggtgatgc cgttggcgcc gtcgatgtgc tgcggcgaca ccttgtcggc gctcatcggg
    43321 tcgacggaaa tgttgctgta gatgacgccg gtttgcaggt tgaaaatgac gttgtcgctg
    43381 acgaagtaat cgtaattcag cgacgtgaac gccccaccct ggcgctggat cgtctcggct
    43441 tcgttggcgt aggcgttcga ctggatcgtg ttgtagatcc acgccggatc ggcattgaac
    43501 gacagctcga cgcgatgacg ctgcgcgggg gcccaagtca gcttcagccg cggatacgcg
    43561 ccataaaatt cgcgcgtcgc gtgctgcaag ttcagcggcg tacccggcac cagcgccgac
    43621 gcggtatagc gcagctccgt cgacaggtag taccacagct tctgctttac gatcggaccg
    43681 cttaggttca ggttgagctg atactgcgca ttgggcggac gcggatcgct ctggtcgagg
    43741 agccggcctt cataaagctg cgttcccgcg cgcgatcccg acgacagctt ctggtggttt
    43801 agataaaagc tcgtgtcgac ggaaaacttg tccgaaccac gcttggtgac caggtttatg
    43861 acaccgccga gcgagttgta ctgcgcgtcg atcgccagaa gctgggtatc aacctgacca
    43921 atcgcgtcga agttaaagtt tgccgaaaag gtattcgaca ccggatcggt gatgtcgagt
    43981 ccgtcgacga gatagcggtt gtgacgcagc gagccaccgg ccatcactgg gtttgaacca
    44041 ccgacgacgc caggaagctg ctgcacgatg ttctggtagc tgcgtccggt tgcgatcttg
    44101 gcctgctggt cggacgtgag cacgcggccc gtcgagactt tttcggaatc gatgcgagcg
    44161 cgctcctcga aaatcttggt cgtctcgcct tcggccatct cggcggcaaa gtcgatcttc
    44221 agcgtgcgcg tctcacccag cgcgagcttg acccggcgct ttatcggctg cagaccttca
    44281 tactcgacgg tgatgtcgta gctgccgtgc ggaattgaca aaaagtcaaa acgcccgtca
    44341 tcgtcggtgg tcgcggactt gctgccacca atcatcgcat ccgaccgcac cgtcactttg
    44401 gcgcccggca tcggcacgcc tgtgcccgcc tctaacacca cgccttgcag acgagcattt
    44461 tgctcgccga cggccagtgc cgcacgctcg gccaacgtcg gaacgaccac aagcgaagtc
    44521 agccaaatga gacgctgcgc gaaaaaaaag cgacgccgca tttacttccc cgctacctgg
    44581 ctctgcgaaa aggggcagtt gacttcgacg ggcacgttgc ttccatcgac ggtcagcgtc
    44641 tgacacggca gacagctcgc cagggcacca ctcgcggtcg cggctttgta cgccgggtcg
    44701 tttgcatcgg gtggcggcgg gctcgtctga cccttcaaga caaactcttt gcagattggt
    44761 gaataggttc ccgctggcac ttccgacgga aggcccggaa tgaccacttg caaaggcgtc
    44821 gtcgttgccg tcggtgcggt ttctttgtag accttgtaga gcggcatcgt cacaaacgac
    44881 ttgccgtcgg tcgaaaccgg caccggcccc atgtcgatgt agggcacgac aagctggttg
    44941 taaaaaccaa gctgcaccgg cagtcgcgct ggcggcgcat tcgacggtgt aagcgccgga
    45001 tcgacaattt gcaaaagccg atattcgcgc gacacgacgg ccagatcctt gtcgattcgc
    45061 ccggtcgcgg tgtcaaagcg cttgatcgac tgacacggca gcgagcttgg tgctgacaag
    45121 tgaatcactt cgacgacggc gctgtagttt agtttacgcc ccggtttgcc gctgctcgtc
    45181 gacagcactt cggggatgtc cgcaaacacc ggatactgac ggtcccagcg gatcaggtcg
    45241 agtcgctcgt cgaaggcttc gtcgcgggtc gcttggcacg atccgtcgaa gacatacacc
    45301 tgaccggcat ttgtcaccgc cagcggatcg cgcttgccat cggtccaaaa cggcgtggtt
    45361 gccgacaccg tcttgccatc cgatcccgtc gacgacactt ttgcagtggt atccgccgat
    45421 cgcgtggtcg caaacaccgt atacggaatc tgcggccagt cgccacggcc tttgccgacg
    45481 gcacgcagcc ggtcgatggt ctttacgcca gtgagaaagt tttgcgcgta caccacgccg
    45541 gcgaggaatt ctccgtcgtc gggataggga ctacacgccg cggcaaaaga caagagcgca
    45601 gcgacggaaa acagtcgtgc agaggcaggg aagcgataac gttctgtcga gacgacggcc
    45661 cttgtgggaa gcatagcggc gcgcatattc ctgcgagccc cgtccgcttt caagcttttc
    45721 cagcacgcag cgacgaaaag ccggcccgtc cgcgctcagc gcagctccag ccgaatcgac
    45781 accgccgtaa gctgggcgac gtagattccg cttttgccgt aaccgcccgg ctcgcccacg
    45841 cgctgcttgt gcaggctgcg cacgtcgcga tccgcaaaca gaatgcccgt cacccgcgct
    45901 cgtgttccgc tcggcagcgg tgccgaaagc cgtcgcccaa gcagttccaa gctgcgatcg
    45961 atccacaccc gcccaagcga catcggccca agcggtcccc cttgccagcc aatcgccccg
    46021 atggtggtga tgaagtggcg atgccagcgt tcggggtcca gctcgacgtc gaccaagctg
    46081 accgcttgtg gcccgccgac cgcccgacgc aaatcgcgct ctggatctga caagcagggc
    46141 aggcacgatg cacagtcacc ccaccccatc ttcaccaatc gattgcgtcc ttcgagggga
    46201 actgcctcta gaacgcgccg tagctctggc tgcgccgccg tcaacataca gaaaatagat
    46261 ttaggctacc gaatcttaga ctgactttga catggcgggc gacgagttgc gggctttttc
    46321 gccaaagccg tgtagtttag aaggcgcatg cagaccccta ccgtacttat caccggcgca
    46381 tcgagcggca tcggcgcgtc gctggccaaa gaatttgcac ggcgcggcgc ccggctcgtc
    46441 ctcgtggccc ggcgcacgga tcgattggaa acactcgcgg ctgatctgcg cacaggtggc
    46501 accgaagtcc tgaccgtcac cggcgatgtc acccgcgatg gcgacatgga acgcgcgacc
    46561 gaggccgcgg aaaagcgctt cggtggtgtg gacattgtcg tcgccaacgc cggctttgga
    46621 gtgcagggcc ggctcaccaa gctgtctatc gacgatgtgc ggcggcagtt tgaaaccaac
    46681 gtgttcggtg tgctgcggac gttttatgcg acgcaccagg cgctcaaagc gagccgcggt
    46741 cggctggcgc ttatcggcag cgtctcgggc tttctttctg cgccgggaac aattccgtat
    46801 gcgatgagca agtttgccgt gcgggcgctc gcggaagggc tcgatcaaga gctggccaaa
    46861 gacggcattt cggtgacaca catcgccccc ggtttcgtcg aatcagagat ttcgctcgtc
    46921 gacaaccagg gcgtactgca cgacacgccg ccgcgaccca aggcccccgc gtttttgttt
    46981 atgaaaactg ccgatgctgc ccgccaaatt gtcgacgcca tcatggcccg cgagccactg
    47041 cgcgtcgtca caggtcacgg caaagtcctc gtcgccgcga gccgacacac tcccggtctg
    47101 ctcaaaggcg ccatggcgct tatggccggt cgcgtcgcca agctgacgat cgacgactag
    47161 tctacggtgc aatcgccatg acagcccccc gcattgtcag tgcccgcgac ggcgagctac
    47221 cgcctaagct atcgcaagcc acttttgacc tgatcgtcgt cggcagcggc gcaggcggat
    47281 cgatgtgcgc gcagcaggcg gcgcaggccg gtctttcggt gctggtgctg gaagcgggtg
    47341 gccatcatct gccaagccag atgaaccagc gggaaggcga catgctgccg cggctgtttt
    47401 acgacgcggg tgggcgatcg acacaagacg gcgcggtgat gattctgcat ggccgtggca
    47461 ttggcggatc gaccgtccac aacaccaacc tgtgcaagcg cgcccccgct gaagtgctgc
    47521 agcagtgggc cacttcgcac gactgtccct cgtggagtcc agagcgtgtc gccccggatt
    47581 atgaagccgt cgaaaccgaa ctttccgtca caccgctcac cgaggaagac gtaaaccgca
    47641 acaacgcgat catgcgccgc ggggtcgaaa agctcggctg gcgcggcgga ctcctacaac
    47701 acaaccgccg cggctgtcag cgatccggtt tctgcgagct gggctgcgcc tacgatgcca
    47761 aggaaaacgc cgccaaagtc gtcatcccca aggcactcct tttcggggcc agcgtcatct
    47821 gcgatctgcg cgtcgtccga attctgcatg acggaaaacg agtccgcggc gtctctgctt
    47881 ctgtggtgcg tagcgacggc agcgacggcg aaaccctgaa tatttcggcg cgtgcagtct
    47941 gcgtctctgc tagcgcggtt ctgtctccgg ctctgctttt gcacagcgat gtacccgatc
    48001 cgcacaagct tgtgggaaag tcgctccggc tgcatcctgg cgtcgcggtg ggcggctgct
    48061 ttgccgaacc agtggaagcg tggaaaggca ttccgcaagc ctatgagtgc agcgaaaaac
    48121 tgtcgttttc tgacggtgcg caagatcgct gctggctgat tccggcgttt gcccatccgg
    48181 gtggttttgc tggactgcag cctggttttg gcaaacagca tgcggcggcc atgcgcgatt
    48241 atgccaagac agctgtcgtc gctgcgatgc ttcatgatga aacaaaaggt gtcgtgtctg
    48301 tcgatgggaa cggacgtgcc aaagtcgatt acgcgctgac cgcttccgat gccaaagcac
    48361 tgctgcgcgg aatcggtgca gcggcggaca ttctgctcgc ggcaggtgcg caggaagtga
    48421 tgattccgtt tgcgcgtccg ctgctggttc gcagtcccaa agagctgtcc gttcttgatt
    48481 cccaccgcta tcgtccgctc gatccgatac tcacttcggt acacccgatg gggagtcttc
    48541 cgatgggact caatccaaca cgagccgtcg tcgatgaaca aggccggcac catcaactgc
    48601 acggtctgta cgtagccgat gggagtctgt ttccgacctc gctaggaggg ccgccgcagc
    48661 ttacgattta cgcgacaggt cgccgggtcg gaaacgcaat tgccgccgat ctgcgtcatg
    48721 ggtaagctcg cgcccgcgat gcgcagaatg cggtccggct ccgttttttt tgctgccctg
    48781 tgtacgctgc cgtttttcgg ctgtgcagcg tggcatgacc tgtttcagcg cgacaggccg
    48841 acggcgcaga tttatgccga agcgctcgcg ctgccacggc ccaagaccac cgatgtcgcg
    48901 attgtgctcg gctgcccggc aaatcccgat ggaaccccgt ctttgtgtct gcgctgtcga
    48961 attcacgcaa cgctttcggc gtatcggcgc ggcgagtttc gggcagcgat ctttgccggt
    49021 ggcgcagccc acaatcgctt cattgaagca gcggtaatgg cccgcgaagc cgagcgtcta
    49081 gggatgccac gccaagcgat tttgcaagaa ggggaatcgc tgacgacgtg gcagaatctg
    49141 cgtttttccc gtcggatcat ggccgagcac ggcttccaga ccgcgctgct tgtttcgacc
    49201 cgcgatcacc tgccacgcgc gcgcaggttc gccgattact atcagattcc gaccgcactg
    49261 ctcggctgtg aagatgaagt aagtccaaac ttgaagtaag tccaaacatg gactcagtcc
    49321 aaatatgaag catttttaag gactaatatc agaagccggt ggcagcggtc cgagcgagcg
    49381 atccatgcgc ttgagaaacg aataccatgg ctcgctgagc ccaggaatct gcacgctgac
    49441 atagtggaaa ccacgcgccc gccactcggc agcttcttgc ggcgacggac ggtacggctc
    49501 acgcgagatt cgatagacgt tgtaggtcaa gatcgtcggc ttgcgcgaca gcacataatc
    49561 gagcggcgca aacttttggt gacccggacg atttgaaacc gccggcacgt tgtgggcgac
    49621 gtactcatcg gacagaccaa agctatcgag cgcgcgaatt ccggcgtaat acacctgggc
    49681 tccagcaccg ccgaccactt ggtaatcgtc gggtttcaca tgctgcgcca gccattttcc
    49741 aatcgctgcg cgatccgctg tgtagtaacg cagatagcca ggggtatcga tgccgcgatc
    49801 cgctccaatg aagtgcagcg cgcggtcatc gacgacatag gaattggccg catgcaggcc
    49861 gaaaaatccc agcaacaccg acgacaaaag cacccgattc tgccgatgca gcagccgata
    49921 gagtccgatc gctccgcaca gcaccgtgaa gggaatcacc ggcatcatga agcgatacag
    49981 ccccataaaa tcgccgccca cttttgcgac atacagacag aacggaatca cccagatgaa
    50041 aaacagaccg cccagcgcca cgtagccttt ttgcagacgg acagacaccg aatcgggcca
    50101 cggacgcaaa aaaccgagca gataaaaaag cggcaagatc acaagcttca gatcgcgcgc
    50161 aaccgcccac agatagtagc ccccttgctg ccaggttccc ttgccaccgg atgacttgat
    50221 gtagaacgta ttgggaacaa agtagccgta gtaccaccgt cgccaggtca tgtgcggcac
    50281 ggtgaagagc aggaacatca gaagcagctg acattcctct tttgtcggaa tcagatcccg
    50341 ttttttgatc ttcaggatga tccgatgcag cgccagaagt gcaaacagaa gatagccttc
    50401 gggccgggtc agcgccgcca gtcccagcgt gattccgatt tgccacagag gcgctctgtt
    50461 tccgttttct tcgtcgagaa tcgcctgcaa atactgaccg agcgacagcg tgcaaaacag
    50521 cgcaaagagc tgcgtttcca agccaccgga agaccagcag gcgtagcccg gaatcccggc
    50581 caaaatccac gctgaaacag aatcccacag cgattccttg tctgcgcgta gcttccgcag
    50641 caccgtcgcc aaataaaaca tcgtgaggat tccgcacagc gttccgagca cgcgcgaaga
    50701 cagctctgcc ggaatcttta gcgcaagtcc cgccgcaacg atcagcgtcc acagaaagtt
    50761 ggtgtaaccc tcgactcgct cgccgatgtt aaagaccagc gcaccgtgct ctaccaagtt
    50821 ttttgcatag acaaacgaga taaacgcatc gtcggtgacg aagttaaatc gaaggctgtg
    50881 gacgaccagc aaaagtgccg cacagaccag cgcggaaagg acgattaggc gggaaggagc
    50941 agacactttc ggaccacctc aaacagcgcg ccacaggcga tgatcacgag caggtgaaat
    51001 atctcatgat agccaaacac gcgcggaaac ggattcggag tctttttcgc ataggtcagc
    51061 gccccaagcg tgtacagaat gccgccaccg acgtgaagag cgaccacttg cggccccaga
    51121 tgacgccact ctccgggcag tcccagaccc ccgatccagc cgagtacaac gtaaattccg
    51181 acatgcagaa cccgcggcgc actgggccaa aagacggtct gcaaaacgcc cgctgcggcc
    51241 cctatccaaa gcaccgaaca gagcagtttt ccaaacgaac caccgacgcc caaaatcgtg
    51301 atcggcgtgt agtttccggc gataaacaaa aagatgttgg agtgatcgag tcgcttcagg
    51361 aatgcgcgcg gaccgggctg ccagtgcttg cgatgataca gcgcgctgac gccaaacagc
    51421 gcgatgagcg cgatgccgta caccaaggcg cacgtccgcg cttcgtctgt ttgcgcaagt
    51481 gaaacgagcc agtacgcaag cggaaacgcc gcaaaaaacg cagcctgatg ggacacgccc
    51541 cgcagcagcg gtttttcgag cggaatcgcg gcggcgaggt gctcgatggt tgcttcgatt
    51601 gcggacgtct tttgcggcct tggtcgcgac atgcaaaaac gcgattccta agcgctcttg
    51661 accagtgtca cgatgttgcg gtttggcggt gcgtgctcga tgacttgctg ctgaagtcca
    51721 agccgaccta gcacaaactc gacggcacga acgcgctctt gcatcgacga agtatcgatc
    51781 ggcagatagg gaatccggaa ctgttcgagc aggagcttga ccattccgtc aatccgcacc
    51841 accgattccc aatccacgcc agcgcggact ccgtcttcac gcaggagcga gcgatggggc
    51901 cgcacgaaaa agaccacgcc ttgcgcgacc catttcatgt actcgcggac ttcttcgccg
    51961 ctttcacccg acatgatgtc gccgagcacc agggcgtgat cggcagcata ggcaaggttg
    52021 tcgaacgcgc gatcagagac aaatcctttt tcttgcagtc gctcgatcgc aatctgccgc
    52081 tcgaacacgc gacgctggta gtcactgacc cgatccatgt cggtgcgcag cgagtcgaga
    52141 tcgatttcaa gttcggcgag caccgaccgg gccacttcgg tgatcatcgg caggccgtag
    52201 cgacgactga cccagcgagt catcgtggtc ttgccggtag cgtgggaacc aacaaagtag
    52261 atgcgcatgg cgggccacgg cctaccaagc tgagctgcaa actgtcaagc gatcccgcgg
    52321 tttgtcgtcg cccgcggggt gcggtggctg cgactaggcg ccttccgacg ctgctttggc
    52381 ttccagaacc cgacgccagc ggcccgaaat tcccatccga acgatgccag cgcgcagcga
    52441 aagaagcagc accgcgagcg caaaaaatgc cgtgcccaca agctgcaccg ccggtttcca
    52501 caccatcacc gcttgcaccg cgcggccttc gcttagcaag cgctcgcgat gcagatagct
    52561 gcggtgttcg ccccaaggaa tcgtctcacg cggggggagc ccctgataga ccggcggctt
    52621 gggcttttcg ggctgcgccg cttcgccacc cgcatctgag gccagtttgc ggctgtacga
    52681 cacctcgctc gcgaccggcg ccatcgcagg ccgatccgcc gaacgcccgc caagcaaaag
    52741 accgccgagc aacagcgccc acagtccgcc cagacacaag ccgccgacca ccagccaaaa
    52801 cagccgcttt tgaccgaaca gccacggcaa aacaaacagc ccgccgacca acagttcggc
    52861 caggacaaac agcaccaaaa acgacgacac cgccgcggca gacgcgcaaa acagaagcgc
    52921 aatgctgacc cgcaaccccg tcgacagacc cagtgcgaaa agcagacgct cggccccgag
    52981 caaaagcagc agaagcacga tcagttcgcc gaccgacact gccggaaagc ggacttcggg
    53041 cgacagctct tcgtaaagcg gcagccagtc gcggtcgtag cgcagctcga tgctggccgt
    53101 cgcggcgggc gatggcagct cgggcagcca cagggtcgaa accccaatgc cgacgctgcg
    53161 cgaaaacgac tgccgatgct gaagctgcac gcgctgcgcg ccttgcgaca gcggcagaag
    53221 cagcgccccg gcttcgtttt tggtcagcag catcggctcg ccctgaaggc tggcgtaggt
    53281 tggatcggcg cgcatcggga cttgcagctc ggcggcgcct tggttgtcta gttccaaggt
    53341 cgtttcgccc aagacatgtc cgtcgctgcc caaaaagaat gtcgtcgctg ccgagcttac
    53401 cgcgtagctc tgcgtccgca tcgcttccag ccgcaccacc cgccacgaaa tcgtctgttt
    53461 gtcgtcgagc aaaaagccct gggcaccgcg gtactgcgga ttcattccgg tttcactgac
    53521 gctgatgcgc tgcaccgccg attccactgc cggacgcagc agcggatgcc catcgagcag
    53581 cagataatgc acagacgcct gcagcggagg cttaaacgtc gtcgctggca agcttccgcg
    53641 cagcgtcagc gtgtgttccc ccggcggcac atggcagccg tattcccccg actgctgttc
    53701 acacgaaatc gccgcgtcaa gccgctgcac ccgctcgccg ttgtggggcg caatcgcaaa
    53761 ccgctgatgc gatcgattgg ggttgcgcaa aagcagctga tagacaaagc ccacaccctc
    53821 gggagaaagc gtcacatggt agcgaccaat cgccgacacc ggcagcgctt tttccgcctg
    53881 atctttggca atgtgaacga ccgaaagccg ctgcttgccg ccttcttctt cgtcgcgaat
    53941 cagctcgccg tctttgactt gagaatcaat ccacaacacc tgcggcgtcg tcacaagctg
    54001 gagccggtcg ccgctggtca gcgccacctg acagcgcagc gaaaaccgcg gcaaaagcgg
    54061 cgtcagttcg tacttgtccg atccaccgcg cgacaaaatc gcatcgcctt cgcagcccca
    54121 aagccgagtc ccgcccgtcg tcgacagcac ctcgaccgtc ggtccggtgc ccgccgtccg
    54181 tcccgtgagc acaagcgata ggttgcgcga ttcaaagctc ccgtcgatgc gcagcgtctc
    54241 tatcaccgtc accgccggac gccccagcat ctgctggtat tccgctagcg gcagagtcac
    54301 ctgcgacggc acggtgttcg cagcgacagg gtccggtttc ttggccggtg gcggtggatt
    54361 tggcggtgcg gcaagagcaa accgcgcaga cagcagggcg cacaaaacca gcagcaccga
    54421 aagcttgcgt cgcgtcgtca gcatgacatc accctacttc atcttccgtc agtacgctgc
    54481 ccgcagcgaa agacccagca agcccggcgc gagcagcgag gagacctgca cttgtcgttt
    54541 cggccccggc aaaacaatcg ccacgatgcc gcctgcgacc gacgctgcgc caatccctag
    54601 caaaagcgat ccgatgccct gggtggcata gatggtctgg caggttccgc ccgggcaagt
    54661 ggacttcccg tcgaccgaca gcgccgaaac gccaaagccc gcgaccacca aaccggcgcc
    54721 aatcaagctg ccaccgagca aaagacgcca cttggggcgc ggcccgactt cggtgcgata
    54781 caccggggct ttcggtggcg gcggcacaaa cggggcaggt tccgcggcgt catccggctt
    54841 ggccagcgtc gcttcgactt gcgccgtctg acccggctgc acgctcagcg tcttttggaa
    54901 cggcggctgt ccgagaaggc gcagctcgac ttcgacatca ccgacgaaac ggctgccttt
    54961 gtagggggtc acgccgactt tttcaccgcc gatcagcacg tcggcaccag gcggtttcga
    55021 cgtgatttcg acgctgccac gggcccgcgc gctcgattgc tggatcacag tgccgacgct
    55081 ggttgccgct gcggcaaacg cttgctcact ggcgcagtcg ctacagcttg ccgtgctgcg
    55141 tcccgcatag tcacccaccg acggatcgaa cgcatcaatc gcaaaatccc agttggcttt
    55201 gccgtcgggc gtcgccgcct ggtttttttt cgctttgacc cgcaagacgt acagcgcttc
    55261 gttggccagg cccagctcgg caagacagct cgccgacgaa agacagctcg ccagcgccgg
    55321 tttcagcttg atcgctttgt cgccgggtac caccacttgg cggtcgcgca gcacctgctt
    55381 gcccaccgcg gcgtgcagcg ctttttgttg ttctgccgtc aagtctccgt cgacgatcag
    55441 caccaaagcc ggcggcaccg acaccaccac cgtgccggtc ttttggttga agcgcatttc
    55501 gaccgtctga cccggcagcg acttgacctg ctcttcgatg cgctgatccg aaaactcgac
    55561 gacgattcga tggtttcctg cttcgagcaa gagcggcatt tgcagcggca gtgcgccaat
    55621 caggtgatcg tcgaccgtca cccacgcgcc ccgcgcacca atcacgctga cttcaccact
    55681 tggcgccgct tcaagctggc cgatccgctg cgcatctttt tgatcgggtc cgtcgttttc
    55741 gccgccggtt tcttgcaaaa agcgccgcat cacatcttgc gcagcgacga tccgaccttc
    55801 tgcttcggcg agcttgccga tccggaacag cacctctggc gccggatttt ttcggaacgc
    55861 ttcttcaaac gcagtcttcg ccgccgaaag gttgttcgcc gccagcgccg tattgccgct
    55921 gctcaccagc gtctgcgctt gctgctgatc ttgcacgctc gctttcggtc gtttttcgtg
    55981 ccgcccgagc gcatccgacg gtgaaaaaag ccccaggcca accaaaacgc cgacgccgag
    56041 ccatgcgcca atcccgaaac gaaactctct catggacagc ccttgacctc gacggtctct
    56101 tgcggacgaa cctgcaccgt ctgaccgccg ccgacgttta cgcggtgacg accgggcggc
    56161 atccagatgt cttcggtctg acagcggcct ttgacgacct tgctgacccg cagccgtccg
    56221 acgcgcggag cgacccgctt catcgcttcc gcaaccgctt tgtgcccgtc ggatttgacc
    56281 ttgccgaaaa agcccggctg ctggattttg tcgtactcgg tcatcgcctg cgcccaagcc
    56341 tcctgcgcct cgaggacttg ccccaggtgg aacgacagct catagcccag gctctgacag
    56401 accgctttgt cttctttttt gcgactgcac agcgtgctgg ctttttcgag cagcttgcgc
    56461 gcttttttgt agtccttgcg ctggaacgct tcgacgcccg ctttgaccaa atcgccaagc
    56521 tgagacggcg cgatcttgac cgtcacggtt gcccccgccg tcactgtgac atcttgctgg
    56581 ccgccgggat agacgatgtg gtgttcaccg acgctgactt cgacgggttt gctcgcgatg
    56641 gtgtgcggtg gctgcttgtc gatttgcacc tgcgtgccag acggtccctc aaaacgcaaa
    56701 aagccttttt ccaccgcggc tggctgcgtc ggcacaggct ctggcccagc gccgccatct
    56761 acatgtttgt cttctttgcc cggttcgctg gttttttcgc ctggcgtcgg cttggcttcg
    56821 gtcgctttgg cttcgctcgg tttgctcgcc gccgtcgccc gactgacgcc cgatttttcc
    56881 agcttggcca acgccaagag cgcccgctcg cgcagtgccg gatcacgatc gcgcgaaagc
    56941 acccgcaaca ccggaagccc cggcgccggt tcgcgatctg tccccgcaag ctgcatcgca
    57001 ctgtcgagcg cctgtgaccg cacaagctcg ctcgggtcgc gcgtcgcatc cagaagcttg
    57061 ggcaccgcct gtttcgctgg ccagcccgaa agagcctcca ccgacgaacg tcgcacctcg
    57121 ggatcgctcg atttgagcgc cgcctgcact tcttttggcg acagctccac cgtgcccgct
    57181 tcccccagct tggtcagcgc cgactgcgcc cagatggctt cggccccgcc ccgtcgcagc
    57241 gcttcttgca aaatgccaag cccggctttg ctacccgccc gcgccagcga caccgccgct
    57301 ttcagccgcg tcacaaacgg cgctttttcg tccgcaacga tcgtccccag ccacgcctgc
    57361 ttgctttcgg gcacttcgtc gctcatttcg accgccatcg cctgcgcttc ggcgtcgccc
    57421 gagccgacca ttttttcgac ctctttggcc tgcgctttgt cacccagcgc caccatcgcc
    57481 gccgcgcgcg ccacttgctc acttgcgctg ccttgcccgg cgtcttgctt cagcttggcc
    57541 atcacactgc cttgcgtcgc cgcgtcgccg cccatcgtct gtccggcttt ggcgattgcg
    57601 cgcagcattt gggttcgcac ctgcgcactt tgatcggtca ggccttcggt cagcgccgcg
    57661 atcgcatctt tgccaccgat ccgtgccagc gagtccgccg ccgcccgccg cacctcgacg
    57721 cggttgtcgt gcatcgcctt gcgcaaaagg ggcagcgctt tttgctggtt cacgtcggca
    57781 agtgccgcca ccgcctgggc ccggacattc cagtcgtcgt cgcccagcgc ctgctcggcc
    57841 cagcttacac tccgttccgc cagaaccagt ggatcaccgt cgcacagctt cagcagcgca
    57901 cctgcttctg cttgtcgcaa aatgccgctg cgttcgccca gtcgcagacc ttgcgacagc
    57961 ttggctgcgt cctgtttgtc gccgcagtgt ccgatgcctt gcgccgccaa gagccgctgc
    58021 ggcatgcccg cgcccgtctg ccacagcgtc gttcgcagca ccggttgacc acttggatct
    58081 tcgatgagac acagcgcatg cgccgccgcc acttgcaggt ttcccggctg caccgccacg
    58141 tcggacagca ggttgcgcgc gcggtcatcg ccaagcgacg ccaatatctg cgccacccgg
    58201 agctggcgcg gctggctcgg tggcccctct ggcaaaattt ggatcagctg cgtcttggct
    58261 tcgtcgtccc cgagcagcgc tttgtgcgac agaatgtcca cccgcgtttc gtcggtcggt
    58321 ttggcctgac caagccgcgt cacaagcagc gtctttgcct ggtcttcccc cagatccaaa
    58381 agtgccagtg ccgcctgaag ctgcacctgc ggatagcgat cctgctcaag agaacgcaac
    58441 agcaagctct tgcccgctgg cgcccccagc ttggccaggg attctccgac ggaaaccagg
    58501 accgttggat cgccgccctc atcggcccga tgcaagagcg ccgccgtcgc cccccggttg
    58561 ccaatcaccc ccagcgcccg cgccgcctgc gcttgcacct gcggatcggc gtcacgcagc
    58621 cgaccttcca gcaagctccg atgtcgactg tcgcggctgt cttcgagcgc cccaacggct
    58681 tgcccacgca cctgcggatc gccgtcgccc agaccttctt gcaggatctt gagcgcgagc
    58741 gagcgcagcg ccaccatgtc gatctcttcg cccttgctgc ggctgcgcag cttgtcgatg
    58801 aaaaagtagc caccgacggc caaaagacag actgctacag gcaaaagcag aagcgccacc
    58861 aggttccgcc gacgcaccct ttcgactttg ggcatcgcag acagcgtcag ccgcgaattg
    58921 agccccgccg tcgtctgctc accgcgctgg agcctatcga gatcggccag cacatcgcct
    58981 aggttttcgt aacgctcctc gggcgccttg gccaaaagct tggccaaaat ggggccgatt
    59041 tccgccggaa tcccccactg caccgaatcg gcaggcagcg gtggcaccgg tttttgcaga
    59101 tgcccgacca gcacttcccc gagcgtatcg ccgccaaacg gaagctgccc actgcacatt
    59161 tggtacagaa tcaccccgag cgcatacaga tcggttcgga cgtcgacttt gccgccgttt
    59221 atctgctcgg gcgccatgta atacggcgtg ccgaccagcg tcccggtgcg cgtcagcttc
    59281 atcgattcac cctcgacgcc gacggtcttg gcgatgccaa aatcaaccaa cttcacaagc
    59341 ggcggctccc cctcccgctc cacaagaaac aggttgtctg gcttcaggtc acgatgcacg
    59401 attccggctt tgtgggcggc ggacaggccg gcgcagagct gccgcatgat ctgcatcact
    59461 tcgcgaaacg acagccgctt cttttccagc aagctcgaca gcgactgccc cgacagatac
    59521 tccattacgt aatagagccg cccgtcggaa agttcgccga tgtcgaagat gtcgatcagg
    59581 ttctggtgcc gcacccgcgc gataaggcgc gcctcttgac ggaagcgctc gacggcttcc
    59641 ttgttgttca tgatctcgcg acgcaggact ttgaccgccg agcgatgtcc gagctgtagg
    59701 tgttgggcgg cgtagacttc gcccattccg ccctcgccga gcagcttgac gaccttgtag
    59761 tcgccgaccg tctgcccgat cgggctgtta gtctcctcgg acatggcttt ccttttctgc
    59821 gagcgctaag agcgacgccg ccgcggtctt ttgcagcact gcatcgctag acgacaaaag
    59881 gggcaaaaga ggttccgtaa gtgactgcgc cttgggaccc aaactgccag ccgcagcgac
    59941 ggcccgcagg cgataagtat cttggcttag caggggcttt agctcgtcga ccatgccgac
    60001 atcgcgagcg cgcgccaggg ccaacaccac aagcggacgc tgcggaagcg gcggtttttt
    60061 ggccagcagc tcgtgcagat ccttctgcgc atcggtcttg cccgccagcg ccaaaagttc
    60121 cagcgcatcg agcgcggttt ccgacagtgg actcgctgcc gcttgttcgc gaagctgttt
    60181 ttccgtcgtt tcgtcgcctt tttggacaag ttccagcaga agctgctgac gcaacatccg
    60241 cggcaccgaa agcgagggtc gccacagctt ttccttgagc ttttgcagcg ccaccttcgg
    60301 cgccttgccc gcttgggtca gcgcaagacc ggcccacagc cgcgcatcgg gtggaagcgt
    60361 ctcgctggct gccgccgagt cgagcaccgc caccgcatct gccgccccaa gccgcagccg
    60421 caccgcctgg gcttcgactg cctgcgcccc gactgccgac tgcgtccgct tgctcaccgc
    60481 cgccagcaag tccacatccc cggcctgccc gatgacatac gccgtcgcca aaagcggtcg
    60541 cagcggcacc gtttcgtccg ccacaagctc cgtcagtatc ggcagcagag accgcttcgg
    60601 caccgcatcg atcgcttgca aaagcgctcg cttgtccggc ggactgccgc gctccgcggc
    60661 ttgcagcacg tccagcgcgc gcgccggggg cgaaccggct ttgtacggtg acacatgccg
    60721 gtggtgccgc gtctgcatca cgatcccgac cgccagcccc gcaagcagca caagcggaac
    60781 cagccacgcc cacagacctc gccgcgacgc ccgcagccgc accggacggg ttgtcaccgc
    60841 ttgcccgacg cttgcttctt tccacagccc acgaccgccc cacaccacgt ccaggtcgtc
    60901 gcgcaggctc tggcaatcgg cgtagcgttc ttcgggcagc ttggccagcg tccgcaagat
    60961 cacccgatcg agccgcgcca tatcgacaaa gcgcttgccc agcttgagcc gcttggcgat
    61021 ctgcgacggc ttttccgcac tttcgagcag atgcgcgccc aaaagcgacg ccagcgagtc
    61081 accgataaac ggacgctgcc cggtcacggc ctcgaacagc atcactccaa cggcataaat
    61141 gtcggcccgc ccgtcgatgt ttggactggc tttgatctgc tcgggcgaca tataggctgg
    61201 cgtgcccatc atcgcgccaa ccgccgtcgc cagcgtgctc agtttttgcg catcaatgcc
    61261 gtcggtattt tcgggccggt catcactccg gatcttcgcg acgccgaagt cgagcagctt
    61321 gacctgagga atcgggcttt ccccgtcgcc ccgcggcacc aacatcacgt tttctggttt
    61381 gaggtctcgg tgaaccaccc cggctgagtg ggcggcaccg acggcggaca gcagcggcgc
    61441 aaacagtgac accacttcat cgtcggaaag cggcccgcgc tctagtcgct tgcgcagcgt
    61501 ctcgccggtc acgacttcca tcacgaaata cggcgcgcca cttggactta atccaaagtc
    61561 atacaccgcg acaatgttcg ggtgccccag ccgagtgacc agctcggctt cgcgctgaaa
    61621 ccgcgccatc gcttcttggt gctgacccag ctcggcattg agcactttga tcgcgacctc
    61681 gcgaccgagc ttttcgtgga cggcggcgta gaccgtgccc atggccccag agcccaggac
    61741 acggtcgagt cgatacttcc cgcccaggat ggaacccggc cccacgctgg cagcagcgac
    61801 ggctggttct ggcatatcca aaacatttta tgtgaataac gtaagccaca caacgcattc
    61861 tcgggaaaac aggcagaaaa gcgcacccaa ccaagtcctc gacagcgggg ccgggcctga
    61921 tctagcctgc gcggctcgca tgagcaaaac gccacagaat ccgacctcgc cccctgaaag
    61981 ccactatcct cgcgacccgg cggcgcgcga gcgagacccc aacttttgga ctgcgtccca
    62041 tggccgagcc gtcgatccgg acgtgcttcc gcgcgtgctt tcgcgggcgg cggcgctgcg
    62101 ggaacaggcg gtgctggtgt ttgatctcga ttcgacgctg ctcgacaaca agccgcggca
    62161 ggcacgcatt gtgcgcgagg ctggtcaggc gctcggtgag ccgcgacttt tgggctgcca
    62221 gccggaacac ttcaccgatt ggtccgtcga caagccgctg cgtcggctcg ggctgcgcga
    62281 cgaagaaatt cccgacctgg tgcccaaagt gcgacgtttt tggaaacagc gctttttcac
    62341 cagcgaatac tgcgtcgacg acattgcgat tgccggggcg gtggcgtttg tgcgcgatgt
    62401 cctcgcggcg ggcagcaagg ttgcctactg caccggacgg cacgaaggca tgcgacaagg
    62461 caccgtggcc tgttttgcac gcgaaggttt cccggtgccc gacggcgatc gcgtccatct
    62521 gctgatgaag ccggacttgg aaacgcacga cgacgacttc aaagtccggg cgcggcagcc
    62581 gctggcggcg ctggggacgg tgattgcggc gtttgacaac gagccgatcc acatcaacag
    62641 ctactttgtc cacttcaccg acgcgcagtg cgttcacatg ctgaccgacg attcggcacg
    62701 cggcatcgcg ccccaccaaa gcatcccgtc gatccgcgac ttccgccgcg gctagctact
    62761 gaaccgttac gtcgggaacc gcgagcccac agacgtggga caggctttcg atgtggcgga
    62821 tcatgaccag accgggcgcg tgacctttgt acgcaccttc gaggagcagt cgcagcttgg
    62881 ctttgccacg gaacgggacg gtcaaaagct gcatctgggt tccgccttgg ccctgctgtc
    62941 cgtagatgag ctggccgcga tagtccgaca agatgtcctc ggtctgcttg accgaagtcg
    63001 tcgtcggcac cgcaatcagc gcgtagtatt cgagcggatc gcgatcaggc gccagctcga
    63061 cttccagcgt aagctcctca ccgacgtggg cttgggtctt gctcaggcca actttggcaa
    63121 atggctgggt cgtcgcttgc agccgcatga tctgaggccg atcgatgcga gcaatcgccg
    63181 ccgcgggcac gacaatttgg gccggacctt tgaccgtctg ctcgaacgaa agcgcgctgc
    63241 cgtccgcttt gcggagacct tgcgccgtcg cttcttgtgc gccgatgaga agctggagtt
    63301 cgataaagcg caccagcgcc atcggcgtcg aatacacccc gccgagcagc gccggaccct
    63361 gaatcagccc cggttcttgc gacaggatca tgttgcggct gacctgccga tacagcttgg
    63421 caaacgtcgc cttgtcgcct ttttgataaa acgccatcgc cagctcggtc agatactggg
    63481 tcgcaccgac gcgctcgaac gcccgcagaa aacggaacgt gtcatacgct ttggcaaacg
    63541 ccacatccgc atccttgccg tcgagatcga gcttgggcag cagcttgtcg ctcgcccagc
    63601 ggatgaccgc gtcgtcggta atgacccgat agacgacctg cccgtttatt tcgaccggca
    63661 ccacgtcttg gccgctcgcg tcgaacccgg caagctgttc ggttttctga cctttggctg
    63721 ccagcgactg ccgaatccgt tccgccatcg ccactgccgt ctgttgtgcc tgcacaaccc
    63781 gcggatcggt cgacgcagcg acttgcagcg accgcaccgc cgcaagcagg ttgcgcgcca
    63841 cccagccgct ccactgtgga ctcgcctgca tccctggata gggacggcac agcccttcgc
    63901 ctttgtcaca gaacgcttcg tcgagatcgc gaacatcttt gtcgaccgaa gtgcgcagcg
    63961 tgtgggccag cccatcgtcg ctgagaagcc cgcgcttgac cgccgccaaa agcgccgacc
    64021 gcaccgccac ccgcgcgctc agtgcttcgg cgtgcccaaa ccaggactcg ctcgtcgtgt
    64081 agacgttcat cgccatgcca cgcagaagcg gtcccgcccc ggcgtacacc cgcgccgttt
    64141 catctgcacc gatggaaatc tgcccgccat cgccgaactg aagccgcgaa aacgtcaccg
    64201 gctgttcgga tatcgtctgc atcgacactt cacgccgatc gtagactttg ccgtcggggc
    64261 ctttcatctg taacgccaaa acgccgggtt ttgacaactt caccgtcagc tcatgcgtcg
    64321 acgcaccggg gggcactgca tactcgctgg caatccccgg ccccgtcacc gaaagctgta
    64381 gcgtcgactg tgttgcgttg gtcgcatcga tcggaatccg cagctcggcg cccggcacaa
    64441 acgtcttggg cagacgcgct tcggccccgg cgacccgctt gacgtccagc tcacgctgcg
    64501 actgtgaaaa ctcgagcgct tgcaccgcga cgacccgcat caccacccga ccggtctgcg
    64561 gcggcagcac gacatcgact tgggcccgac cgctcgcgtc ggtgacaatt ttttggcaga
    64621 acaccacttt tttgtcgccc tcgcgcactt cttctttcgg cgcctcgtct tcgtccccgc
    64681 caccgtctgc ctcaccgccg ccaccgcccc caccaccgtt tttcgacttt ttgcccatct
    64741 tgcccagccc gccgccatag cccatgccgc cgccgcccat gccgcgaccc gaaagcccaa
    64801 gcccgcccgg agccggtggt gccgaaggtg ctgcgcccat cgacatcgac ggtgccgcca
    64861 tcatcttgcg cggcgcatat tcttttttgc tttcggccat ttcttcgcga tccgccgcgc
    64921 gatagtccga ttcatcgagt tcccagccgg tgttgtccga ccacgacgac accgaacgcg
    64981 acgcattgcg cagcgagtcg cccaccgcgg aatgtaggtg cgtcgccggg ctgcgcgacg
    65041 gcacccggtt gtcatagact tccaaaatcg ccgacagcgc gacgcccttg ccgcggtctt
    65101 ccgcctggat cgtgacgggc actttgccct tcggcatgcc ttccgcctgt gcgctcaggt
    65161 tgactttgag ccgcgacggc ggaaacgcca gcgcccagcc ttccctgaac gtatccgcga
    65221 cgaaaccacc gatggtcacc agcgtatacg gccctgcgac gtcgacatct attttgcgtc
    65281 ccggcacaaa atcccccgac accgccactt gcttcgcggc atacgttccg tcgctgtttg
    65341 gggtgtggac gaacagaacc ggcgcgctga ctttcgccgt cacttcgatg ctgagcttgc
    65401 ccgagctggc gatcagcgaa ctgaccgaaa acggatcgga gccctgcttt ctgctttcga
    65461 caaacagatc gcgacccggc acttgcacgg tgttttcata tggagcgacg ccgactttgt
    65521 ggccgtaact gacgccgccc gccgccgcca ccatgtcgcg ctcgacgtgg cttgagcctt
    65581 caaactggtg acgcagactg ccgcgcggtg acaccacttc gatctggaac ggccccgagt
    65641 gaccaccgac gttcagcgtc ccgagcagct ggccccgttc gaccatcatt ttctgcgacg
    65701 gacccgcgag gctgccgtta cagcccggca gcatgcagcg cgagtgaacc ttcacctcac
    65761 cgctaaatgg c
//
LOCUS       DAORGN010000004        60451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_151_length_60451_cov_5.992913, whole genome shotgun sequence.
ACCESSION   DAORGN010000004 DAORGN010000000
VERSION     DAORGN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 60451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60451
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_60451_cov_5.992913"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKL17_00855"
     CDS             complement(<1..447)
                     /locus_tag="PKL17_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK43300.1"
                     /translation="MAKIVVIEDEVDLQQILAWNLRNAGHEVQTSTHGGEGLSLCEEF
                     APDLLILDRMLPDIPGTQVLLKLRQNPRTAPIKVVMLTACGEESDRIVGFELGADDYV
                     VKPFSVRELLLRISAVLRRSGKEENAPTPSRPELPDDESLLTIGALR"
     gene            743..1270
                     /locus_tag="PKL17_00860"
     CDS             743..1270
                     /locus_tag="PKL17_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43301.1"
                     /translation="MASAAVAACTLALSVRSERIFILLCVVWLGLTLLTSLIFIPISL
                     LTDARFVEMSGDLRSRTYDMTVMSGLREKKLSGSFDDFASVLLRSELVSDGLAAPFRQ
                     QYIFLCWHKERRDPLGPIVFRRVSSGGEFGNQKAVAADLAEAMGLPLIDATAGRGSGR
                     QSTDHMPSMAAVDVV"
     gene            complement(1274..2278)
                     /locus_tag="PKL17_00865"
     CDS             complement(1274..2278)
                     /locus_tag="PKL17_00865"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK43302.1"
                     /translation="MIVAVTGATGFVGTNLIAELLSQGHKVRALLHAGSRAEQGLRKR
                     LPPGSDIEIHHGDVLSPGTLQRAFSGAEVVFHLAAQISISRHDAEGVLRTNVEGPRNV
                     ASACLENGVRRMIHFSSVHAFEVPPHDEPMDETTPLVHGDAAPAYDRSKSLGEREVMR
                     AVEKGLDAVILNPSGIMGPNDHGPSRIGRTLLDFARGRFPMVVPGIYNWVDVREVVSS
                     AISAVEKGQRGHKYILGGEVGSIRKLAELVAEAGGKRAPRYSAPLWLLAPVAPVIETV
                     AHLTKTSPLLTPQSIQILQSPCDFRSDKAMRELGHRIRPLSETVGDAVTWFRETGHLP
                     "
     gene            2379..2549
                     /locus_tag="PKL17_00870"
     CDS             2379..2549
                     /locus_tag="PKL17_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43303.1"
                     /translation="MSSGRRETPNPNADPADVAREILQGGQTSESDTNDSDDDGSEER
                     GQSGTKGTGSAG"
     gene            complement(2608..2757)
                     /locus_tag="PKL17_00875"
     CDS             complement(2608..2757)
                     /locus_tag="PKL17_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43304.1"
                     /translation="MKTVMAMVLGSLFLANAAIACPGEGGAHKDQKTADSKGKTETKD
                     KKTTT"
     gene            complement(2877..3923)
                     /gene="rtcA"
                     /locus_tag="PKL17_00880"
     CDS             complement(2877..3923)
                     /gene="rtcA"
                     /locus_tag="PKL17_00880"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNK43305.1"
                     /translation="MEHAAGPIELDGGHGEGGGQILRTALSLSLHTGRPFRLRDIRKH
                     RDKPGLRPQHLQAVLAAQRIGAAFVEGAAIGSTSLWFEPKQAVAAGKYVFDVGTAGST
                     LLLLQTLLPSLLFASGPSQLVLRGGTHNVKAPPFEFVDRVFLPRLLQLGYRVSMQLVR
                     PGFYPLGGGELTVSIVPSTAQHRLQLLGRSPHRKLTAEVLLSRIPDHVARREQAVLQK
                     RFGLSPLDCRVRALTDCLGPGNAVLLQVDSDDGCELFSAIGERGVKAEDVAAACADEA
                     QAFLDADVPVGEHLADQLILPLALGQGGAYLTTKPSLHTLTQIDTVRAFLPVQIQVQQ
                     QDALAYLIEVNTPS"
     gene            complement(3962..5056)
                     /locus_tag="PKL17_00885"
     CDS             complement(3962..5056)
                     /locus_tag="PKL17_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43306.1"
                     /translation="MSLFRVVLPSLLLLSACGKGDDAPPSFATDKPRFFQGLNLLSDD
                     TRAAFPDFERRWDQIAKDTLTKLDNGEIVEEVDQKNAARALLYAAFALVAGQHAVEDG
                     YLKLEQLTRPPRYSKGDDQDEQRARMNKALEWLRRAADLDPTDDRIASVIRSCGFTLQ
                     TIDGMYTADVLLGINSAASQSWFDTFTAMMLVRDPNLHPGYAPHVEQLISVACGTSRF
                     NCSLGPQPLPKTGERTLTREVMGPLLLADLLAKRGESQLQRADLNPAQAGTLVPEAAM
                     RFKVADQFLSAASQAAMDPAVSHFPVRSRLPERSARLKLLIDAAASRMGAGQGSELPD
                     PSYYKNRSYLDAYQCVACHTAGPSTQGFPK"
     gene            complement(5159..5240)
                     /locus_tag="PKL17_00890"
     tRNA            complement(5159..5240)
                     /locus_tag="PKL17_00890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5204..5206),aa:Leu,seq:tag)
     gene            complement(5324..5396)
                     /locus_tag="PKL17_00895"
     tRNA            complement(5324..5396)
                     /locus_tag="PKL17_00895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5361..5363),aa:Lys,seq:ctt)
     gene            complement(5491..6567)
                     /locus_tag="PKL17_00900"
     CDS             complement(5491..6567)
                     /locus_tag="PKL17_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43307.1"
                     /translation="MNKLTFASLGLAASVIASVALFNCNNDPMGNTPTDTADMAVGST
                     DMATGSGDMALTNPDLAGVAAPTKVTGFKQPTSAFWDAATSSWYVSNVGGATVTDPKT
                     FSTNMTAFISKVPANLMNPNHAWYPNTATPRLNAPFGLRGGGGKLYVGNIDKLWAIDM
                     ANPTTVAPLQSATVAAGGLASLAGYPTFMIDVALDSSNNAYVADATGRRLLKFTAPFA
                     AGANPTTIVAADTFAGTSGVYIDGTKVVIAEAGINQVIMQSGGISTCNLDGSGRTRLV
                     NSTKNSLAYQGIEKDTVANKYMIASPGDKIVYSVDPSTGAQTILRNVAQDGATTATDI
                     GWDPVGKVLAVPDTGADVVYFYKL"
     gene            6747..8246
                     /locus_tag="PKL17_00905"
     CDS             6747..8246
                     /locus_tag="PKL17_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK43308.1"
                     /translation="MANTSLRISPRVHLWLLCGTVLSALFGFVYARLVCQYICALPTD
                     RLARNIAIVSLAQFALRHHLLTRPIPWQSTRTTARTLFLRSVLSWFVAGALAIGLHFA
                     LYPDFPVGSHFKFALAYWALGGGLCTQLEYLLFEQEFPAQAVDGANPLRERLRDRLIE
                     GYAIYTLTPALVLLLTLLRVVRELGGTTQQVIESALLMGVFAGMALLAALLFGRSLRK
                     DSERLMEAVRKVGQGDFSPRAKTTRADELSQVAVGINEMASGLLLRERIRDAFGRFVS
                     PQVATEFIETYAKSGRSAELGGKRRDVVILFSDLRDFTPLSESLPPERLIEVLNGYFA
                     EMVAAIHQQHGIVDKFIGDAVLAVFGLVDSPTGQAGENPAISAVRAAQTMQRQLRLYN
                     DTLRQQGIRLSAGIGIHAGEVIAGYLGTHERMEYTVIGHNVNVAARLESKAREPLPTL
                     LFSDEVARRIGHTLPVRAAGEVQLKGVTSAMPVFTVDEPPTLPIGSSGQ"
     gene            complement(8240..9241)
                     /locus_tag="PKL17_00910"
     CDS             complement(8240..9241)
                     /locus_tag="PKL17_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43309.1"
                     /translation="MRLTAWLTKLVCTSVFSSMMLLGCHQSSQNVRPDYATPYGQDTS
                     GYPQSEMQSGTGGSADEAYGGYGGAPTPSSPSLALPEKSRREAVAPRQVERPGLGTEF
                     GESRYSPVRDVPFERGSSSPLFTGTIHYNDARGVEAQRERLLRRHYPGEPIWRSYVGN
                     RPIPWSGVTVSVVDGNGNPLPAYHLQSHVLVVGSAGQHYSIVVENNTGQRFEMVASVD
                     GQDVVDGRSASLEKRGYVVDAFHRVRIDGFRTSMTDVAAFRFGSVRNSYAARTSEFGD
                     RNVGVIGIALFGERGAPAPAVGPDLDDEARRRESADPFPGRFSPPPPPPPPPPRPYY"
     gene            9321..11342
                     /locus_tag="PKL17_00915"
     CDS             9321..11342
                     /locus_tag="PKL17_00915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43310.1"
                     /translation="MPSETPAPDLCADLRSALLSTDLAKLEALWQTILNSPPTEKRRA
                     AAILAKHGLSDRAAQLHEQLGDLPSAQALYQQAGLLTDAGRVSEQLGDKRLAEALYRQ
                     SIVEHRADTAAQSPDGAVADRSSPALRLGQLLLRQGRAEEAIPFLQAIRRKLLTNNTP
                     LPLLDEVEPALLHALVQVQLPEAALSLLQSYSRRHPDAPPTVAEWLLTAPQPSVQERM
                     LLGRYRLQRLIGAGAMGRVFVAEDTASSRSVAIKLLPMPIDSQARDSKWQMFCREAQI
                     LQTLRHPNIVAVHDFSESAGVLVMELLRGGSLGEQPLPLSLVKTKRVLRDVCAGLSAA
                     HAASVLHRDIKPHNLFLDEAHNTKIGDFGAALLAQLGATQTESLVGTLAYMSPEQLEG
                     QKLTVASDLYSLGVTLFQLLTGRLPFVGPDWVSQHLRQPPPDPRSLRPALPAAWADLC
                     LQLLAKSEADRPSTMDELAQRITQLPDAETAQQAGTLEGKLPSADDGLLPHLETHSLH
                     PIDGTSPTLLYQTGHSAVYHVVSAKLARGVLIERFDREKLMTPTGQHHLRWLRAMARL
                     GGPGLQRVLRIMLDGHSPEVHFEETVGLPLGANGPPSRQDEAILLATLHAIHAEDEVH
                     GNISDAVIVEKYGAQLQVVGRGPLGWKSPAPTPKDEERTLLAMLRSRKT"
     gene            complement(11346..12053)
                     /locus_tag="PKL17_00920"
     CDS             complement(11346..12053)
                     /locus_tag="PKL17_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNK43311.1"
                     /translation="MAQLKKRSKTSASPPKEPPLNSPFLAARKELWGLLDKAPVKEPP
                     KAPPPPKLTTSEEEQLSDEELFRREMQGTNRLAVQGRERPVEQERAARQRLSEDAEAY
                     AQLSDLVSGSGSFDISDTDEYIEGIAHGLDRRLLYRLRRGDFAVQAHVDLHGLNREEA
                     RLRVETFVHDSKLNGRRCILIVHGRGLNSKDQIPVLKDAVRLWLQRGRIAKSVLCFAT
                     ARPTDGGAGAVYVLLRR"
     gene            12134..12607
                     /locus_tag="PKL17_00925"
     CDS             12134..12607
                     /locus_tag="PKL17_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43312.1"
                     /translation="MPHEMVDRSPPYCRICEGVEGTSLPTDCPGEKLTSAQVSGVHFK
                     TLDYRDGRWDDPAVFDWKPAAMPTASNFGGPSIVVTGDDGSTTVSGSGGSRHITTIIP
                     TEPVDEPEENGGAPSLIPVPIPIPIPIPIPIPTGGSSGPAEEEEGDPSYDVRVNK"
     gene            complement(12619..14055)
                     /locus_tag="PKL17_00930"
     CDS             complement(12619..14055)
                     /locus_tag="PKL17_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43313.1"
                     /translation="MHWLRFSLLAVAALLLGGAEPPSALPRGWHCLVKLTDGPHAQAN
                     VSLTFPAPTTPQVCADMPSAGRFVRDIQQIFSDGRALPLVRDGDCFRLIDAKAVPVRL
                     RYKYDLAAAADHFNNADLVQRHEQAMTFSEEAVLLRPDPLPLPDSPSLPSPSIDVEFE
                     LPTGTALTVPWQKLPGPGLRYQLDAAQYDGGSYVTFGAVRPLGTLALAHSQVELHLVG
                     KSRASDLELRTWIEQASRASERLYGPQMPSNVHIVIVAWPGQTELGSFGTVLRPLRPS
                     VLIYFGADATRFPLSDDWVATHEIFHIGNPLMRYKVPWFVEGFTTYYQDILRARLGAL
                     TAKEAWSDLWDGFRRHCQPNGTSLETESANLHKTYRYTRVYWGGACLAFLLDVEIRKK
                     SQGKRSLDDVMRSLRKQSLHTRLDEESVLEGLGQASSPALVRRYLRERNALPLRETLT
                     LLGIEPVGDDSVRLRDDAPLSALRRAMF"
     gene            complement(14059..14832)
                     /locus_tag="PKL17_00935"
     CDS             complement(14059..14832)
                     /locus_tag="PKL17_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43314.1"
                     /translation="MARFRTIFGASMALLFASSMSALAQYPTRPAPPYVPPQPVAQPA
                     PQYVPAQPQPAPQYAPQPQYAPQPQYAPQPQYAPEPQYAPQPGYVAEPEVRVEMAPPA
                     PQMEVRPVAPSQRHAWIPGHWAWRMGRYVWVPGYWDMARPHHVWVRAHWVRVGHQWVF
                     APGHWEPLRQPQYMDGPPPPSGVIVQSAPPPPMEEVVPMAPSVQHEWVGGHWTWNGVR
                     WIWTPGHFVARRHGFGWQRAQWVQAPGGWRYVPGHWRRM"
     gene            15112..15624
                     /locus_tag="PKL17_00940"
     CDS             15112..15624
                     /locus_tag="PKL17_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HNK43315.1"
                     /translation="MRLYSGKIPSIAQDLIRKLKEDGDIEVSDVSEAQLDVEAVLKEY
                     LRLERELTEKAKDYMEKRRLPYEQLPKIKRAMAEERDIGIGDESVSYIANQILEAFMH
                     SRFVEEVFADDADMRKKIQGILRKHMQVDADIDHEVRRRIKNLQEGTATWEIEYQKVM
                     EQLRRQHKLE"
     gene            15676..15891
                     /gene="infA"
                     /locus_tag="PKL17_00945"
     CDS             15676..15891
                     /gene="infA"
                     /locus_tag="PKL17_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNK43316.1"
                     /translation="MSREDLVEMNGTVTNVMVGGNFEVVTPEGHKILARLSGRMRRFR
                     IRVVLGDQVLVGVSPYDPTRGLITRRT"
     gene            15951..16403
                     /locus_tag="PKL17_00950"
     CDS             15951..16403
                     /locus_tag="PKL17_00950"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNK43317.1"
                     /translation="MLKVGDTAPDFSATATTGKPVKLSELRGKRVVMFFFPKAMTTGC
                     TIETRLFRDHYTEIAALGAEVIGVSVDSNEKQCEFASREGVSFPMIGDSQKTIGKSYD
                     VMWPLLGVTQRVTYVVGPDGRIEAVFHHELMVGRHLEDVKRHLQSKAA"
     gene            complement(16471..17784)
                     /locus_tag="PKL17_00955"
     CDS             complement(16471..17784)
                     /locus_tag="PKL17_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43318.1"
                     /translation="MTSAEPGAPISPDAIDPELVNLKVPPARRHPLVAIAVMVVSLLL
                     LYKMRGDITYALLPEQPTDVGTPEGAATGKLAGQGDKFVKLSGLPDHRNSVSYDPKGG
                     RGRVQVFRLLGSANRLLVARKASLSADAANEFVGRLRPISDVWFVDALRTYFKQTQAL
                     RAIDLDKLKSAPAGKLTLPYATLDRAGQPLSVSREQDLLIDVLVEDDVRVLLSKEKFP
                     SEPDARYEVERLGLPHGPGIETRDGFGYVLRLPAKGPDRQKMLAQIDSMGIWLWHRIE
                     TYRVPSALLQLGPTGIEIPGPDALQQPVRYRTAAVAPAPAAPGSPAAPTIPTAPANPT
                     VAPQPTPAAAPAATATSAPAPPAAAVDSKLLTAVREPSTLLLWSEVQAVQVSEPLTIP
                     EDAFVLFDGETPQQVKWAPPVAALLGLFVLLNLYYLARLRSRPEE"
     gene            complement(18015..19022)
                     /locus_tag="PKL17_00960"
     CDS             complement(18015..19022)
                     /locus_tag="PKL17_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNK43319.1"
                     /translation="MNLTHVALSLLLLAPACAKNAPSNLASSSMSGDTVVAMIGSQKV
                     TMAELDKAAGRELFEVRERALDNIINERVIKAEAKKVGLDEDAFLKQQVEKRVPEVSV
                     DEARKFYEENKARLPPQIGGQPFEQVKDMLVKGLTGQNRQRAVGDVIEEIKSKAGVKV
                     LLTAPKVQVATEGPAKGPKDAKVTIVEFSDFQCPYCGRGRKVMEEVIAKYGNKVRVVF
                     RDFPLSFHDKAQKAAEAGQCANDQGKFWEMHDWMFDNQSTLDEAALKDAARKLGLDGT
                     KFDECLTTSKHAAAVQKNMKEGSEAGVKGTPAFFVNGVFLSGAQPFEKFKTEIDRALA
                     E"
     gene            19086..19787
                     /locus_tag="PKL17_00965"
     CDS             19086..19787
                     /locus_tag="PKL17_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNK43320.1"
                     /translation="MSILSRLGNLIKSNLNSAVDKLSDPAKEIDLLIQEMEDELSQLR
                     VTLRDQLVQEKLAQKKVDEEYRNVQKWQDHAERAVRAGDDELAKEALRRRVEAEAKLE
                     AAERTHAEQSRHVQKLSDDLKSGDRKLTEIKSRKESLKSRARAAKQANDPEGTAMDRF
                     NRLVAEIETQEEQAAAMAELVPELGSQRQNDRDRETEERFNRLLGPGKDSKESDIDSR
                     LAALKAKQLPAKSDS"
     gene            complement(19784..21751)
                     /gene="nadE"
                     /locus_tag="PKL17_00970"
     CDS             complement(19784..21751)
                     /gene="nadE"
                     /locus_tag="PKL17_00970"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNK43321.1"
                     /translation="MRLLRLGLGNVNTTVGATDANVERAIGICHKLADQQVTIAALPE
                     QVIGGYPPEDLLLWPGFVSAQAEGLFKLAQATAQVPMAIVCGVLVQSLEQVYNCAAFL
                     YQGKVFGVVPKEKLPLYNVFYEARTLARGFPGLYAEIDLGPCGQVPFGDLLFDCDFGR
                     VGLEVCEDIWSPDGPMRRRSYQGAELIVNLSASPFRIGVQSTRREMVATRASDNNVTV
                     VYVNAVGANDGLIFDGGGFVCQNGRFLHEAPRFVEGGSVCTVDLRRTTRLRTESTTWR
                     MDREEFLSRTLAQSGSGVRRDSSTCGAQVISVGAIGSASKLCYPFPSHRNFFLPSPSV
                     PADPRRTFCEELLDAMAMGVGDYFEKTGAFKQIGVALSGGRDSLLCLLIAHRYVTRRH
                     LSQGEAVVKQKVADMLRAFYMPTRYSSSATCSAAEIAATELGIPFVILSIEDAFAREV
                     EATQKMLHPGEELTQLTVQNIQARLRAERMWNWSNAVGGLFLQTGNMSEKAAGYTTIG
                     GDLMGCLAVIANLPKTVVNYLLDYLLETEKLDGIRQTIAIPATAELAPDQQDERDLMP
                     FPVLDACIALHAAEKLDAKELADVLFDMFPEYPADRLRGFANKFVMLFARAIYKWVQA
                     PLSLHLGNLDLERERCLQLPVVTRSEWQKRA"
     gene            complement(21800..23248)
                     /locus_tag="PKL17_00975"
     CDS             complement(21800..23248)
                     /locus_tag="PKL17_00975"
                     /inference="COORDINATES: protein motif:HMM:NF036998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43322.1"
                     /translation="MSLSALTLDVYQLTTLIAHAADGRLSDGPLAMSFFFRKLPKQRN
                     YVVSCGLRSIVQHCQSLRFSAEELALLDSHPILGPALSTKTGQLVRDALARLDGFDGE
                     IDAIPEGTLAFAGPAQREDGSPLLVSGTPLFAYTPLLQVRTDLTLCKLIETPWLSRIN
                     YMSMVASKASRVVAAAQHDGVLRSVIDFGQRRTHPMAAIDAAYAAYIAGCSATSNLAA
                     TYCHGIPSVGTMDHFAVQACERLQTPVAETEMHFFRRFCELFPGHATLLVDTYDTFRG
                     IENAVRATDGKLAGVRLDSGVTPESVRRARKLLSDLGAPHAKLFLSDGLDEYRVRDLA
                     TAGGDGFGVGENISCSPDAATGIGAVGKLVQNATGKLTMKLARGSGKATLPGRLQVYR
                     FPDHDLLTLHDEPARPGGTPLLQPLWRGKKLVSPLPSLTETRDHVSAQRTALPAHLRK
                     LEVATSGSAEAPWPIKLSNRLVHVIDELLSTM"
     gene            complement(23245..23919)
                     /gene="pncA"
                     /locus_tag="PKL17_00980"
     CDS             complement(23245..23919)
                     /gene="pncA"
                     /locus_tag="PKL17_00980"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HNK43323.1"
                     /translation="MTDGAQSDVWTIDREHDALLVVDLQPDFMPGGPLAVAEGDQVVL
                     PIAALVPRFHFVVATQDWHPKNHVSFAAQHGLSPFSSIPLYGADQTLWPVHCVQGTPG
                     AALHQGLPLDSVGVILRKGAYPHIDSYSAFRENIGPDGKRHTTGLGALLHARRIRRVF
                     VCGLARDFCVGWSALDAIAEGFSAVVIDDLTRAVFPDKEEETSARFSAAGVLHGRSSR
                     LQGGAP"
     gene            24185..25552
                     /locus_tag="PKL17_00985"
     CDS             24185..25552
                     /locus_tag="PKL17_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK43324.1"
                     /translation="MGNPPPDPPPETLNLTDASPKLMKNGGGTAVTLKGTGFKTGATV
                     TIGGVAAQDVVVVDSSTIQLKSPLKAATCGTVTVVVTNPDGNAAMRGDLITFLTNRVS
                     FGVPNPITMGTYPRQVLAVDLNKDGKVDLVSANSGQAAQALVYRLGNGDGTFGNPTSY
                     TAGSSTFGVAAVDLNKDGNLDLVTANSNSNDISVRVATGAGTFPTASTQYGAGADTRA
                     IAAADLTGDGAADLVAVNYGSGNISIYKNNGDGTFAAQSNLSVGASPQHVILTDLDKD
                     GKLDVVTANANGNNLSVRLANGTPSIFGAAASVAVGSSPWFVTAADWNGDGKMDLAVV
                     NNGMTASTYMVSILLGDGKGSFATPVNIDIGNQTAPRGAVATDLNKDGIMDLVVAGYG
                     SNTLPVLIGKGDGTFEPVLSVTGPAFPQSVDVADFDGDGMPDLVATSSSTGSQNVATL
                     LGQCK"
     gene            25643..27262
                     /locus_tag="PKL17_00990"
     CDS             25643..27262
                     /locus_tag="PKL17_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNK43325.1"
                     /translation="MIAFTNVTKQYGGQILFVDASFQINPGERVGLVGPNGAGKTTIF
                     RLIMGQETADDGSVERPRKLTLGYFNQDISGMRGRTVLQETVAGAGEVSTLGEEMKVL
                     EEQMAQGCDDLDAVVARYGEVQARFMELGGYDIDARAHTILAGLGLTPQQIEADVGNL
                     SGGWKMRVALAKILILQPDVLLLDEPTNYLDIESILWLEDFLRSYPGSVVMTSHDRDV
                     MNRVVKKIIEIDGGQIRSYTGNYDFYEQARQQEAARREAEYERQQAMLAKELRFIERF
                     RAQAAKAAQVQSRVKKLDKIERVEPPRRLIEREFDFRKPARSGDDVIRVAGLRKAYGD
                     KVVHRGLDLLVRRGERWAVMGENGAGKTTLLKMVAGVLNPDAGEVTVGASVTLGYFAQ
                     HQMEQLDGTATVIDELQSHAPTTGIGVLRNLAGAFGFHGDDVEKPVRVLSGGERSRLV
                     LAKLLFDAPNLLVLDEPTNHLDIATKRALVKALSSYQGTLLFVSHDRAFLRAIATRVL
                     ELTSNGPHIYGGTYAEYVSATGHEAPGMRTN"
     gene            complement(27300..28193)
                     /locus_tag="PKL17_00995"
     CDS             complement(27300..28193)
                     /locus_tag="PKL17_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43326.1"
                     /translation="MMSPHIRSVLSRSSLLLGFVGAVQLLSVGCGGPFTPLETESEGT
                     VSETTAASVWESIGVGVNYRGTGNAGENVFIGYAGYNVSDSQSKAWTDALYAARLSAL
                     GVGHLYAVRGPADVTYVRREIGNTKLIAHLLPKIGSRTKLITIASHSSGSYVANELLG
                     FLYTSKFDSTGKTFDKTVYFNLDGGGGITWDAVNRLYRTFFAYSKDGRSGTLSPNAAG
                     MISLANSFVPRAGSIVVPADAAGCASGASWCLHMTLINQRPHNPSGTDLRDYSDFSAR
                     PVQTSYLDQAWPLLDGLAKRP"
     gene            complement(28205..30142)
                     /locus_tag="PKL17_01000"
     CDS             complement(28205..30142)
                     /locus_tag="PKL17_01000"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNK43327.1"
                     /translation="MMQLLSRAVWHCPLFFVGMAACQEPSELPPIHYEEPRTPCAAHS
                     PLRQAYFGDLHVHTSLSFDAYAFDVRVTPEQAYRFARGEEIALPPNDGMGNGTRKIHL
                     SRPLDFAAVTDHSEFLGEVQACITPSAAGYGSLTCQRYRMGGNSAITNLGFKLAIEDQ
                     RFEDICPPDGSGCAELLSVTWNRLQQAAQTAYDKSESCGFTSFIAYEYSGAPGLSTLH
                     RNVIFRNDRVPLPITHYEEPTPQGLWQALDDACRNARTGCDVLAIPHNPNESNGKMFR
                     IEYPDGANLDEQRRLATLRQRMEPVVEIYQHKGDSECRNSLSGVLGAPDEQCDFEKTL
                     RKNVPDCGEGTGSGGTARTGCISRRDFVRYALLDGLSEQLRIGVNPFPLGFIGSTDTH
                     NGSPGNAEESGFVGHRGTDDDTPEKQLGGGGITIGGMEFSPGGLVGVWAEENSRPALF
                     DALRRRETFGTSGPRITVRFFGGFDLPDGLCSDPRLVEKGYQNGVPMGGTFAGKAAQA
                     PRFVVQALRDPGDGTRPGTPLQVIQIVKGTLTAQGPITKVFDVAGNRANGASVDPTSC
                     VQKGEGADLLCGSFRDPEFDPKQPAYYYVRVLENPSCRWNTLLCNALPAAQRPAPCLD
                     ASVPKTIQERAWASPIYYIPG"
     gene            complement(30139..31146)
                     /locus_tag="PKL17_01005"
     CDS             complement(30139..31146)
                     /locus_tag="PKL17_01005"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNK43328.1"
                     /translation="MKKDSTPSGSMGIWLVFLWIACAISPSAALAHSFAPTVVTLSEE
                     TDGTFWVLYQAPANGETMTPSFPLHCSLLSRDGTAIPEESELSGRILSCGAMGLRGHP
                     IVMKGSDLGETLLTIHFRNREVHSALLHREPHLIPPLHTTNTPTLALTLRRFFGLGVH
                     HILSGIDHLLLLLGLLLLVPSGSALLLTVTAFTVGHSATLALAALGVVTPPPALVEAL
                     IALSVAFVAKELVCVNRSQPMLRPLLLAVLFGLLHGLGFASALRETELPSGQLLLSLL
                     SFNCGVEAGQLVFVLALWLPARFLQRQQRLPWVGYVMGPLAMAWTIERSLILFGKGLP
                     S"
     gene            complement(31143..32069)
                     /locus_tag="PKL17_01010"
     CDS             complement(31143..32069)
                     /locus_tag="PKL17_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNK43329.1"
                     /translation="MRTAQPNSHLAALLFGASFALASPRSLENDAPMRRFLQTLLREP
                     LLHFALLGGLLCCAYWFVRPVPREQIVIPKELVSARRGDLLRRLGRPPTAAELAAATS
                     EYVESEVLYREAQRRRLGDGDIIIRRRLIQKMEYLADALIPPSIPTDEELVALRDATP
                     ARYLLPSRIALRQVFVGFDKHKDRAEIVAAALHDRLQTGTDTALLGDPHPLGANLPLH
                     SESELAQLFSASWAAAAFALPDGNWSTPLRSPFGLHFIQVTGRQAGAMPSLSALRDRL
                     RLDYAEQNRAARRQRAIGALLPRYEVVQKGGT"
     gene            32091..32873
                     /locus_tag="PKL17_01015"
     CDS             32091..32873
                     /locus_tag="PKL17_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43330.1"
                     /translation="MRRYWPLLCLLSFFPGPLSAHPQFALSTVNRYARLVMMPGRVRL
                     DYSLMVGEVPAERIKRQADLDRNGELSDSEQKQLVDSLVATVQRDVSVQMDGQKISAP
                     FVSQPLTLKSPKVESTPFALELQTDLPIPNQPSPHTLVVDDRADIPPVGEIELRIEEG
                     PGVELLSAESGSTKPQSNQSSSPNPIQNAPTLLTETKVPTLFRQFGPPRSLLSDRSVT
                     MRFQLRKQSPQASPRRFTYGLGVFSLLILCGLGLFARRFLRS"
     gene            complement(32875..34011)
                     /locus_tag="PKL17_01020"
     CDS             complement(32875..34011)
                     /locus_tag="PKL17_01020"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNK43331.1"
                     /translation="MRSLQTEFRPIDSPSASPIFVIGTGRSGTTLLRQMLNAHPNIYI
                     AHESGFYSLLRLAPKQIPLRTFLLRYFDTFSFAWMRLDPRDVFASLPAQLVLDDARVA
                     YAEILKAKATQRGKRRFGDKNPLDTQNLARIFSDFPDARVIFIVRHPVPTVQSFGQMP
                     FGTGSPFINGLLCRIQYSHIKPFLDSILEVRLEDLSRDPRTTMQSILNFVGEPWDESV
                     LDHVQNAQTDDLPPSPWFVGATKRAPNQREGAFRAELSAAWTEIVEWQNRETMQRYDY
                     PTQTSAKKPGLLRCLATLIGSVPTMTESMLRLLWVNHKIVQHLQGKHRLDPQIAQRDN
                     LMLNPAAWRYYPEIDIPEVPRPLLPDGKNQPTRSEVPLSFSQSA"
     gene            complement(34096..35658)
                     /locus_tag="PKL17_01025"
     CDS             complement(34096..35658)
                     /locus_tag="PKL17_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43332.1"
                     /translation="MKLKGHGLRISALLLGANAFVLLVPLLSIVALRFYENYLVRQTE
                     RQLIAQAIAVGEMWRELWLREQGIDPALGAPPFRHPGKESEPFIPIEPTSNLRRGVLP
                     PQPEPLRVAPPQDSPVLRAGKKLEPLLARMQVYNLSAVRVLDSQGCVVATTRGESGLC
                     LDNLPEVESALVGRYASVIRERISDEPLPPFSDVRSRGSVRLFVALPLFSDGKVIAVV
                     RASRTSLDARTSLWHNRRGLLFAVSATVFAAVFVSFLFAALIVRPLRKITTSAQTIAQ
                     GGTAPPLPMSLFTPSEIQRLSAALDTMTRKLRERADYVSQLAANVSHELKTPITAIRG
                     AAELLRDEWEGMTEAQRRQFADNIDADAERMQRLVMRLLQLAQIENAPEPTETIVVAD
                     FFAALRNRYPGVCFVLQSPPDKVLISGDHLRSAVVNLIENALRHGEGRPVTVRVSSDA
                     GRLCIEVEDHGTGISPANQPRLFQRFFTTQRDSGGTGLGLAIVRAVAERHGGEVTFST
                     SPQGTTFTLRIA"
     gene            complement(35655..36341)
                     /locus_tag="PKL17_01030"
     CDS             complement(35655..36341)
                     /locus_tag="PKL17_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK43333.1"
                     /translation="MNRILVVDDDGHIRDVMRFALQQGGFQVVEAKDGADALLRFDAG
                     GIDLVVLDIVMPESDGLEVCRRIRAKSRTPIIFVSSRDEELDRVLGLELGADDYLVKP
                     FSPRELLARVKAVLRRLFPESGAPEKRDVQKLGALEMDLGRHRCLWMQKEVILTVTEF
                     ALLQSMLGSPGRVFSRGELVDRAYGHGHVITERTVDSHVRRIRQKLSAQGADPIETVY
                     GLGYRLKESL"
     gene            complement(36351..37622)
                     /locus_tag="PKL17_01035"
     CDS             complement(36351..37622)
                     /locus_tag="PKL17_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43334.1"
                     /translation="MLRNHFIFGLSLALCGMLAIGSSSPVRAEQPPSGNPVATLPLAE
                     VLRLYKASESKAESPSAKPPIRATISKFELSGRLLENAIDVSAHVELSVLEASGWVSA
                     ALFRKDAALRLTKLPQIQNGVLTVVDGMLVFMTDKPGTYSFDVGFLLSAQVKGARRSA
                     DVQYASAALAVLKLRFDENLFTLGNSDRIEEGDGYTLYPTNNRFEVLWDRSSKAAVVA
                     KQSAVRPPIEPIINRAHASVVSTLEGRRIIRLMYALQFEGARSIDFVIPEKQRLEKVF
                     VNGASVPFKANGKTVTLPVQPARAGDESAKVELLVVEQQGGYALSGRLDYTFPATSWN
                     INDLYVTLSLPQVFNYHWEGGSLATMEEGDEVEYTQKIPTPGKSIYLHQQLLSNAATV
                     RIAYTVDLAGSYYRAGREAEKVADPRTLRSE"
     gene            complement(37612..38859)
                     /locus_tag="PKL17_01040"
     CDS             complement(37612..38859)
                     /locus_tag="PKL17_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43335.1"
                     /translation="MKRILSLFRLLSRYHFASSLLIVMVVSTAISTQMTDRRNFASSE
                     LYRDVMDRWGAPIVQPGPSVRYVPSGAVFNSLTALPLQSQEVTVDAAMNYRKRGLVYF
                     SGFDFSFRGDYAAENDQGRDIDIAFVFPINMEKNKVLLSNLLFSVDGQPTKAELESGS
                     DRLLWTGRLKAGAKVTFRVEFRGRGLDAFTYSLDPAAPVRNFRLGMHIRGGDNFDYAD
                     GVVPATESRSKGDEVWLSWNYQSLQSGIPVGVILPSEKSYDHLIATMVRRGWAPFLLF
                     FFGLTALCLYFAHPLKIYESYLIAASYALFFVLLAYFAAFMSFYPAWGLSLLVAGGLL
                     LFYLGRVLPAGARRYVLGLIVSSLCVPTAAVFMQGYTGLVYTLEILALIATMMVLTTR
                     PAVRELIERLLTPAAAEGDSHAA"
     gene            complement(39018..41258)
                     /locus_tag="PKL17_01045"
     CDS             complement(39018..41258)
                     /locus_tag="PKL17_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43336.1"
                     /translation="MSLIGKRLGNYDIQGKLGEGGMGIVYMGVHPQIGKKVAVKVLHP
                     ELSDKADVVTRFFNEAKAVNDINHPNIVDIIDFGEATVDGTTFKYIMMEFLDGESLSA
                     RMRREGVTIRESVSILAQCCSALAASHAKGVVHRDLKPENIFLVNRPSDRNYVKLLDF
                     GIAKLTTEDGAMSQKTRTGMVIGTPTYMSPEQCDGKGNIDHRADIYALGVVMYELLTG
                     KVPFPGEGFGEVLVAHLTREPDPPRTVNQNVPAELEAIVMRCLRKKREDRFQSMNDMQ
                     LALVDPMPHFQSFMQGQPAPRANGPVPLPGSGSVVMSAAMMPSGPMNLMGGPMSSGIV
                     NPAQSGVMRPPGASAAMPAMMPQAAMSNPGQYGQPNWQQMQQMQMQQQMQMQQQMQMQ
                     QMQQMQQMQMQQQMQQQQMQQQMRAGNPMASGVVQPGMIPGHPMASGVMQPGQMQSLQ
                     MPSMTPGPSMQMGAVQPGSGPRAQTPSTPQPSAPVESIPSPAAQSGARSTLSGANGET
                     AGRRKPATVLAGFAAAMLVGSLSMFGVKQFRGGAPTVVAAPQKAVAVHISVKTDPPDA
                     EIEQSGPDGKNVVKAPYTFDVKKDSGEFSLKIRASGYQEESRTIHPSEDQNIAITLAK
                     QEKPSEPSMDTGSTPKGKTEAVAAKEPKEVKEPKEPKEPKEGKAAKEPKEPKEGKAAK
                     EPKEPKEGKAAKEPKEPKEGKAAKEPKEPKEGKAAKEPKEPKDGKAVKEKGGKKKKED
                     LMAPVF"
     gene            41518..42213
                     /locus_tag="PKL17_01050"
     CDS             41518..42213
                     /locus_tag="PKL17_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43337.1"
                     /translation="MSQLALALAIVSTLPAALQATPAPTAAVPGQPAAYVPPGAPTTP
                     PTEDVAAKELSKKFTAFIKCEEGRKDPLMHYCALTRLSSDPIWTPGQPVSYAGLTVFV
                     KTGADLKKSVSEPAALSVLHLGPSSAKLLSLGAASTPDGKATAQTLQKALAGELKEPP
                     VLPQAVTSTWKNDAHGGRTPLKIDRLYADITSSPPVRIFRAESPHGAAFVTVETLPDG
                     QRFSVFPLSAGIQ"
     gene            42261..42836
                     /locus_tag="PKL17_01055"
     CDS             42261..42836
                     /locus_tag="PKL17_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43338.1"
                     /translation="MRRIHPQTQLRAGQPSTAQFFAAIGLLLLLGAGCKKEPPLVIDV
                     RVDLASPAAAADLAKPPALPVVSVPPQSGEEPTIELSGVVRIGPSGRPRYPVSVIVAS
                     GDCTSPSSRLLRRVPLSDTDSFFVVVMADPGEQLTVCAASEPSPGLPTPIWVKSDPIT
                     VGPASEQAVSNLSLVLRSAEPKTFSTAARKK"
     gene            42842..43402
                     /locus_tag="PKL17_01060"
     CDS             42842..43402
                     /locus_tag="PKL17_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNK43339.1"
                     /translation="MAYFRHETAWVDEGAEIGEGCRIWHFCHVSAGARLGRDCVLGQN
                     VFVAKTAVLGSCVRVQNNVSIYDGVVLEDQVFVGPSAVFTNVVNPRAHVSRKSEYRQT
                     VVGRGATIGANATIVCGHQIGPYAFIGAGAVVTRDVPAHQLWVGVPARFVGWVCACGE
                     RLPDDSLHCAGCGSGYVLRGETLCAL"
     gene            43390..44094
                     /locus_tag="PKL17_01065"
     CDS             43390..44094
                     /locus_tag="PKL17_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43340.1"
                     /translation="MRAVVPLLSASLFLSVVTFQGGCASSQRIADRSQLQSELELAFD
                     DGQPAERPLLPGGDGEWLIRFDPTLPAYKPQRLRLLVAQPGKIRFAIYRSDESGRPGA
                     KLTSLERTYEVNHTSSGMDGKWVLEPIGDLPVLSGTLWIGISVPTTDPTAARLWVSRK
                     ESGQVFQRDSEPGTALQSTKLPMTPMVRLLLSAEQPVPQPAAKPATETDPKKATTGAA
                     ATTPAQAAPQKDKDAK"
     gene            44084..44518
                     /locus_tag="PKL17_01070"
     CDS             44084..44518
                     /locus_tag="PKL17_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43341.1"
                     /translation="MPSEFHKDRPSFEESSEDRGFRERLEQLLPDVVRRAISAGSTAI
                     TSPEETIRRLVSEFNLPREVATYLLVQASSSKDELLRVVGQEVRRFLETVNVSGELQK
                     LLTSLSFEIKTEIRFIPNDAAVKPDVKNKVTVKRGKDDSSDP"
     gene            complement(44527..45132)
                     /locus_tag="PKL17_01075"
     CDS             complement(44527..45132)
                     /locus_tag="PKL17_01075"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HNK43342.1"
                     /translation="MSQQDAKQMLVQAYEQVLKAYALMGYPTQEDDNFRETAERAAKA
                     AQEMVRPVAEIESEISDLLQKTFPARYDQMVISKHNICFGLCPHHLLPVIYRVSAAYL
                     PKDRVVGISKLSRLIRLLARRPVLQEDLTHELAELLCNRLDSRGAGVYIEGLHMCMAA
                     RGVEAHEARLVTSAVRGIFIEQPSTRQEFLDLVTATPLQLL"
     gene            45358..46197
                     /locus_tag="PKL17_01080"
     CDS             45358..46197
                     /locus_tag="PKL17_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNK43343.1"
                     /translation="MISSHRLALAVTALLTLPSVASAAPPHDFSIYAIRMGGDAAQAK
                     PYVDRFARHLETVTGWPAASSNGAFFTSRKEAVAALDAQKPGWAVVEPSLYFELRKSH
                     KAVAVAQVVSSDLNTAQLHVIVKDPAIKSLSDLSGKRVWTQLADAGKYLSNVVFDGKG
                     AAETRFQLKQVGVVTKGIRAVIRGEADATLVDPDQLVEAKKMEGGADLRSVYTSEALP
                     GLLVVAFPQLAAADQKKLSTALIGMCATANGGPVCKEMHIQKFEAVNSAQLSTTEKRY
                     EKP"
     gene            46194..47021
                     /locus_tag="PKL17_01085"
     CDS             46194..47021
                     /locus_tag="PKL17_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43344.1"
                     /translation="MNTRFVTAFGLIFLSLTAACPPAAEVKTAAPKAALSDAELPSDP
                     AALIKLADEQLALGGVGFRNAQLAMEKALSNAEFAGQKSSFDAHYRLAQAAAGQCTLD
                     EGAACQAGLPKALESARRAVEISPDRIEGHYYLAQLIGFSAQTQRGADVKPMLTQMVG
                     ESELAIKIDEKFDSGGPLRMLGTVYTRAPAPPVAIGDPEKGVQLLTRAVAAAPEHPLN
                     VIYLAEAQVADERYQDADASIQKARKLLSDAKWSSRRAEWKEVLAKVERKLKAKQGQ"
     gene            47115..48179
                     /locus_tag="PKL17_01090"
     CDS             47115..48179
                     /locus_tag="PKL17_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43345.1"
                     /translation="MRATVARFGVGVGLLLAGCALAPPTHPSGLPRVPRYTNSIQLDF
                     LLSSYRAVAEKAAMSSESAKLLDEACLGWERAVLDLHGARLYPSILRQTPETLMLEQV
                     WQDLPKTVEKYPRPAVCDNLKIPVSTSGMPKEVTLGVVASTVPPAASAMGSGDAASPT
                     PPPVAPSPTAPPADGAGIMPPPAISMPSTASSEPLTPVARERVALQRLRIALSLPPNP
                     PANLPSAAIDPTLPLGLPGTVDLPVLRELAESDLPTVKLRARYHLLGLCTLAVETADR
                     NRTPISSASQAAIQPADPVVCGANSPNESLRAGQRRLLRSLLSAWRARYSEPMADFVT
                     SLASFVSRDNPVLDGPRTSR"
     gene            48410..50401
                     /locus_tag="PKL17_01095"
     CDS             48410..50401
                     /locus_tag="PKL17_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNK43346.1"
                     /translation="MRKPPLRSPRDYLAQPETQHSYLGMEPARGRTAVAGGESFAELF
                     EASLRSEKRAVAKRDPEVGELVQGEIVQIGTEYAFLDIGGKSEAMISLDELRGEDGEL
                     TVHIGDKLEGHVIGSVGKDGGLLISGRLQKGAGLSDQIRTAYRQGTPVQGLVTGVNKG
                     GLDVDLGGVRAFLPSSQIELRYCQDPSIYLGKALALKVSRISEESRQVIVSRRAVLEI
                     EQRALSDESRARLKIGERYTGTIVAMQEPAALVDIGGLDGSVPTAELYQAARSAGRDE
                     NSIKIGQRVDVEVVKLEEVRPGHDHLHHSFQGNLRIGLSLKGLLADPMAEALSTLSEG
                     ERVRGRVVRLEPFGAFVEIRTGVEALIHISLMAERHITHPREILQLGQELTATVVSLD
                     RERRRIGLSLIEEARAAQAQVAQTLKAGDRMKARIERVEGNGLIVRVMVPDVLPTTFP
                     RGLIPNAEVGVPRGTDLKKLFQIGREVMAVVMSVDGEGRVRLSIRQLTEAPPAPAVEA
                     APVVTEVATAKAIEAAAVSHEAPAVTASTETPKSKPKTTRAKKPKVADESSTASAETP
                     VQPAAPTAEAESTKSPARKTTRTKKKAEEPAPAEVVVAEVAPAEAPAQKATKAKSSTS
                     KKTKAAAEETPKAEPAKAPVLEAPAEPVTATKKRVRKSA"
     gene            50561..51961
                     /locus_tag="PKL17_01100"
     CDS             50561..51961
                     /locus_tag="PKL17_01100"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK43347.1"
                     /translation="MRSRIPVLALSLLGCLGLGCPPAADQRPDTVPPLDNGPAKEPIP
                     EVCDDPVLMAPLTAEHANYGRAFTRYNRLEQRYKAKLQARELQAAYDSLMSLGRLSLR
                     RLNKPDVAIAHFSEARTLAAQIGEPAKEADALVFTAQAQVRRTLYSDAVTTLGQAQAI
                     YKKQNMPLAEARTMALTGIAYHHLKNPQKAVALVGQADNTVRTVKSDDRQVKMAVGRT
                     LMAIGSARNLLGETRRAEDAFQEALAAFVSSSDFPEQAQALEEIAEIHEKIGNHEKAA
                     AYFQEAYAVWHKLGNTDGQADTLIDLGDELFQLGQKDLALNSYMKARALYEQSGQEGD
                     SSAALGRMGKAYAALGQKAQAFASFFQALSLARASQRPTRLAAVLHDVSATYSTMGDY
                     EHAVRCSQRAFQFWQELGEQDDAVRALVEVGDNYLKLGDLRGARDNYDRALGICRALV
                     DLSCEATVRKRLDHAQ"
     gene            51992..53329
                     /locus_tag="PKL17_01105"
     CDS             51992..53329
                     /locus_tag="PKL17_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK43348.1"
                     /translation="MNRESPPVVVLGCGYVGTRLAQSLLADGVTVRVCARRIALLEPL
                     RARGADVHYLDGGRPHQFAPALAGLLRPVVVYSIPGVPGLPQGEGVRRAATAAQRAGA
                     SAFIYLSSSAVYGRSELPFTAEWVDEDSPIAIGDADAMVRLADEAALQSIAQSGLRTV
                     ILRLGAIYGPAVDEFHAARGARQRLRAGQYKLWDGGRHYFSRIFVDDLVRIIRTAADK
                     APPGSTYVVGDDTPCLQSEYGTWLAHHLQLPMPPEVDAARAGGPRNIIRGRRLRNAKL
                     KQELGLTLLYPGYREGELAIDAVEQGAPLPPLRLSDQTPLVPNSATKPPASVPVPSQA
                     DAPATIAEAVSPAPVPEAVTVAATPSPWPKPVDTSDLGLLADATARGVFAIRLKAGEV
                     AERGPAYVLLCGHAKAQLGGASHVFAAWSAVPAGTAITATEDCVLIQITAPQN"
     gene            complement(53348..54166)
                     /locus_tag="PKL17_01110"
     CDS             complement(53348..54166)
                     /locus_tag="PKL17_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43349.1"
                     /translation="MAATLGHALRGLGLWALVSSGCAPDLRLILLNIPDKAEKLYVMT
                     HTEIRGSDRMSIAESKTSAPQPLSVSGLSSAERVQYTYGLVLSGQPSIQSSGQVKQTT
                     VSIAATQGSTILSIGDSSSVTTEESFVDVQLDLAQLAAPPRPDLTQKSLDKDVFIYSA
                     KRYLTLDPRTMQPIFELDVLGWNFPPTATVAVTVLKCASTGQPLALVGDTTASSSSQL
                     IFRTRTQGMMPGSRPQPLDSCIIDIVAKPDSTIKMEVTASGSVLDSWMQGPPVS"
     gene            complement(54182..54877)
                     /locus_tag="PKL17_01115"
     CDS             complement(54182..54877)
                     /locus_tag="PKL17_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43350.1"
                     /translation="MRDRELVVFALLLKAVIVATPARAAEAQEPGPAEKSLTEKASPS
                     SETGSADDHARRAQSLFKEQRFSDAADELKRAYAQAPNPLFLFNAGQAYRKALRPIDA
                     KDMYERFVAVAPNSPLVPEAKGYVQTLDILIGEQQAKQQAELTLLQEQQAKQRAEENL
                     SQKETELAEQIRKREEAQRQLDRVKNPPLYKKPWFWGLTSGVIVGGAAIAVGVGLGLT
                     LYKTDGGKIVLQY"
     gene            complement(54977..55777)
                     /locus_tag="PKL17_01120"
     CDS             complement(54977..55777)
                     /locus_tag="PKL17_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK43351.1"
                     /translation="MFSDNQAAWNARVDVHEQSRFYDVEGFRAGKSSLTPLEEELLGD
                     VSGKSLLHLQCHFGLDTLSWARRGAKVVGVDFSSKAISLARKLTQDVGLSDVARFVDC
                     NVYDTRQHISETFDWVFSSFGVIGWLPDLRPWAKVVYDSLKPGGSFFLIEFHPYVWMS
                     QVGPDLSIRYSYFNAGPITELGSGTYAEREAKITYREHGWNHPLSDLMSALISAGLRV
                     DHFAEYDYTPFDIFPGLVARPGNPPGFWFAAHPGMIPLVFSLRVSRPA"
     gene            55904..56470
                     /locus_tag="PKL17_01125"
     CDS             55904..56470
                     /locus_tag="PKL17_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43352.1"
                     /translation="MLVVSVRLLTPWMDRYGSTDEEVRASYAGDDLVPFPRSFVNHAV
                     TIFQSPDQVFPWLLQLGADKGGLYSYTKIERLLGCPLTNADRIHPEWQNLAVGDLVKM
                     CPHDPAPPPYIVAQIIPNRALVLGHKENGNWVDLWQFVLAPTKEGNTRLILRTRTMMV
                     GGMWDVIHPGVFLMERGLLLGVKERAEK"
     gene            56532..57461
                     /locus_tag="PKL17_01130"
     CDS             56532..57461
                     /locus_tag="PKL17_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK43353.1"
                     /translation="MPSVLFSAVRRYVIALFFLSAATAKADPIPESAKRIRGQVTSLQ
                     GAPMAGVEVRAQDGGSQLLGSSKTDAQGTFQIPLPQPAADVTLVVEASGYRRFARSGF
                     VPDGQSKSIQLGRTIDAQYLSSLLKETDPNRFHAQAADLLNPAPGTTGEGRPQEILLP
                     FLTPLCARLRPLVTGKESQLKERTLAAEPSAVMQILAYCSEPVDDSLIDAWRTGSGSF
                     AARPKIPCQSETLDGAIQKWQALHLQNEGIRPGAEPPMQIRKQTSPSNDRALVSVSVE
                     YAHWGYRNTLVLVRKKNLWVVRLVLSDEIWHGT"
     gene            57655..58629
                     /locus_tag="PKL17_01135"
     CDS             57655..58629
                     /locus_tag="PKL17_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43354.1"
                     /translation="MFESPSKSASRPLPAALSAPTAAKTRAPQPQAPVSPAMQPAAKG
                     MPAPGAIEKALGHWAEPSAAAKTALPQRPAAAALTSGAAASPGFMKFAAGNLKQGRIP
                     FPQEGGGPDCQGPPELGTLSARYESNGDPGTVSTGNGDIGGVSYGSYQMSTASGSAGQ
                     FVSGSPYANEFSGLQPGTPAFSDKWRDIATRDPAGFQAAQHEYIKENYYDVQSGRLTQ
                     DIGLDPNKHSNALQQVIWSTSVQHGAYSDIVEDALAGRDVGKMNDTDLINAIYAERGR
                     TDENGTLVHFRDSSWDVQQGVANRFRNENTDALGMLANEQTCAPPQNP"
     gene            complement(58678..59739)
                     /locus_tag="PKL17_01140"
     CDS             complement(58678..59739)
                     /locus_tag="PKL17_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43355.1"
                     /translation="MMRRTSACVALASYVVASVLGAVSVPSAYAADPAPPAQPAMGQL
                     GGPGPCSKGSTPSGFASLGQKVWLFQPTGSGTPRTGGTCNDKTRPVVFIAAGFSALIP
                     EAYQHLIDDMVSNGYMVVYVNYTLMYAPSIVYPQVDEGFRYGAGWLNFVSGNRMDLAN
                     IGIWGHSYGAGMLPWLSNKAAARGWGSSSLWLSMNATAWLFWVPLTGPIHLPKNARVQ
                     VIAYDADTKADHRVGIDAFSAFDIAASQKDHIQIRSDKGTVADHFTPVTWDLSWLPHD
                     HLDFYGVYRNYQALGDCARAGKHCDADLSYMGTWSDGSEAIRAVVSDSPVDMGPPAAL
                     ECDNTSDPARVFNPRFCVP"
     gene            complement(59768..60286)
                     /locus_tag="PKL17_01145"
     CDS             complement(59768..60286)
                     /locus_tag="PKL17_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNK43356.1"
                     /translation="MTRICLSTVATLAMLMCGTACAATATDDCKGLKNAVVIRTDFHR
                     LALCKDDKVDQSYSVSIGRGGVGKQKEGDNRTPLGSYALGMPRASTDFVIFIPIGYPT
                     AAQRQAGYTGGSVGIHGPKRGFGWLGGLLNFRDWTRGCIAVNRAEDIHDIAAWVNLNH
                     PPIVEIRGTSAQ"
BASE COUNT        12657 a        18606 c        17614 g        11574 t
ORIGIN      
        1 gcgcaacgca ccaattgtga gcagcgactc gtcatcgggc agctcagggc ggctcggcgt
       61 tggtgcgttt tcttctttgc cgctgcgtcg cagcactgcg gaaatccgca aaagcagctc
      121 gcgcaccgaa aacggcttga ccacgtagtc gtcggcgcca agctcaaagc cgacgatgcg
      181 gtcgctctct tcgccgcagg ccgtcagcat caccactttg atgggcgcgg tgcgcggatt
      241 ttgacgcagc ttgagcagca cctgcgtccc cggtatgtcg ggcagcatgc gatcgagaat
      301 cagcagatcg ggcgcaaact cttcgcacaa agacagacct tcgcccccgt gggtggaagt
      361 ctgcacctcg tgcccggcgt tgcgcaggtt ccaggccagg atttgctgaa gatcaacctc
      421 gtcttcgatc acaactatct tcgccatgcg agcggtctcc gtggcccgca gtgtaggtcg
      481 actgctgggt tgccggggac tgcggaagca tcgaaacgat gtgacatttt cgtaacgcct
      541 tttcgagagc aaactgcggt agactgaagc acactggccg tcgcttgcga ggggaagtaa
      601 gaggacggta gaagcgatgg ccagcatggg agtgcgctag atgcaagact ggctggattg
      661 tcgcctctct gacgaagctg tcgttctccg ctccccacag cgggttcgga aaccggcact
      721 ccgaacgatt cgggacgcga gcttggcctc ggcggcggtc gcggcctgta ccttggccct
      781 gtcggtgcgc tcggaacgaa tctttatcct tctgtgcgtc gtctggctcg ggctgacgct
      841 tctgacttcg ctgatcttta ttcccatctc gcttttgacc gatgcgcggt tcgtcgaaat
      901 gagcggcgat ctgcggtcgc gaacctatga catgacggtg atgtcggggc tgcgcgaaaa
      961 gaagctgtcg ggcagcttcg atgactttgc cagtgtgctt ctgcgttccg agctggtgtc
     1021 cgacggcctg gccgcgccat ttcgccagca gtacattttc ctgtgctggc ataaggaacg
     1081 gcgcgatccc ctgggtccga tcgtgtttcg tcgggtgtcg tcaggcggag agtttggcaa
     1141 ccaaaaggcc gtcgccgccg atctggccga agcgatgggc ttgcccctga ttgatgcgac
     1201 ggctggacga ggcagcgggc gacagtcgac cgatcacatg ccgagcatgg ctgctgtcga
     1261 cgtggtgtag cggctacggc aggtggccgg tctcacgaaa ccaagtgaca gcatcgccga
     1321 cggtttccga cagcggacgg atgcgatgac ctagctcgcg catcgctttg tcggatcgga
     1381 aatcacacgg actttgcagg atctggatcg actgcggcgt cagaagcggc gatgtcttgg
     1441 tcagatgtgc cacggtttca atcaccggtg ccacaggtgc gagcagccac agcggcgcac
     1501 tataccgcgg cgctcgcttg ccacccgctt cggcgaccag ctctgccagc ttgcgaatcg
     1561 atccaacttc gccgcccagg atgtacttgt gcccacgctg gcctttttcg acggcagaaa
     1621 tcgcgctcga taccacttcg cggacgtcga cccagttgta aattcccggc accaccatcg
     1681 ggaaacgccc acgcgcaaag tcgagcaggg ttcgcccaat gcgcgatggt ccatgatcgt
     1741 ttgggcccat gattcccgac ggattgagga tcacggcatc cagacctttt tcgaccgcgc
     1801 gcatcacttc acgctccccg agcgatttgc tgcgatcgta agcaggcgcc gcgtcgccat
     1861 ggacaagcgg cgtggtttcg tccatcggtt cgtcatgcgg cggcacttcg aaggcgtgga
     1921 ccgaactaaa gtgaatcatc cgtcgcacgc cgttttccag acaggccgaa gcaacattgc
     1981 gcgggccttc aacattggtc cgcagcacgc cttcggcatc gtggcggctg atcgaaatct
     2041 gcgccgccag gtgaaacacc acctcggcac ctgaaaacgc gcgctggagc gtgccggggg
     2101 aaagcacgtc cccgtgatga atttcgatgt cgctccccgg tggaagccgc tttcgcaggc
     2161 cctgttctgc gcgtgagcca gcgtgaagca aggcgcgcac tttgtgtccc tgtgagagca
     2221 gctcggcaat caagttggtt ccaacaaagc ccgtggctcc cgtgactgcg acgatcatgt
     2281 ggcgaatggc tccttgtccc aaaaaggtcg gaaggacaca taaaacctca agttgacggg
     2341 cacttccaag cgacgaaata gtggtgtacg gttccactat gtctagtggc cgtcgtgaaa
     2401 ctcccaatcc aaacgctgat ccagccgatg tcgctcgcga gattttgcag ggcggacaga
     2461 ccagcgagtc cgataccaat gattccgacg atgatggttc ggaagaacgt ggtcagtcgg
     2521 gaaccaaagg caccggctcg gctggttaaa aaaaaagcgc cacccagtca tcctgagcag
     2581 cgctttttgt ctacgaccta gccgaagtta ggtcgtggtc ttcttgtcct tggtttcggt
     2641 cttgcccttg ctgtcggcgg tcttctggtc tttgtgggca ccgccttcgc cggggcaggc
     2701 aatcgcagca ttcgccagga acagagaacc aaggaccatc gccataacag tcttcatggg
     2761 gaacctcctc tacttcacgg ggacttttcg ctgcgacaaa ccacttgccg caacaagtgc
     2821 attacatcgg atgcctctga catcgctagg atttcagtgg gtttccgccg ccgcgtttag
     2881 ctgggcgtgt tcacttcgat caggtaagcc agcgcgtcct gctgctggac ttggatctgc
     2941 acgggcaaaa acgcgcgcac ggtgtcgatc tgggtcagcg tgtgcagcga cggtttggtc
     3001 gtcagatagg cgccgccttg gcccagcgcg agcggcaaaa taagctggtc cgccaggtgt
     3061 tccccaacgg gaacatcggc atcgaggaag gcctgcgctt cgtctgcaca cgcggcagcg
     3121 acgtcttcgg ctttgacccc gcgttcaccg atcgccgaaa acagctcgca tccatcgtcg
     3181 ctgtcgactt gcagcagcac ggcattgccc ggccccaggc agtcggtcag cgcacgaacc
     3241 cggcagtcga gcggtgacaa accaaaccgt ttttgcagca ccgcctgttc ccgcctggcg
     3301 acgtgatcag gaattcgcga aagcaacacc tcggcagtga gcttgcgatg cggcgaccga
     3361 ccaagcagct gcagccgatg ttgcgcggtc gaagggacta tcgacaccgt cagttcgcca
     3421 ccgcccagtg gataaaagcc ggggcgcaca agctgcatcg aaacgcggta tccaagctgc
     3481 aaaagccgag gcaaaaaaac ccgatcaacg aactcaaacg gcggcgcttt gacgttgtgc
     3541 gttccgcccc gcaagacaag ctgcgacgga cccgacgcaa aaagcagcga gggcaaaagg
     3601 gtctgaagca gcagcagcgt gctccccgcg gtcccgacgt caaagacgta cttgcccgct
     3661 gccacggctt gctttggttc aaaccacagc gatgtggaac cgatcgcagc gccttcgaca
     3721 aaggcagcgc caattcgctg cgccgccaga acggcttgca ggtgttgcgg acgcagcccc
     3781 ggcttgtcgc gatgtttgcg aatgtcacgc aggcgaaagg gccgcccggt gtgaagagac
     3841 agcgacagcg ccgtgcgcaa tatctggccg ccgccttcgc cgtgtccgcc gtctagctcg
     3901 attgggcctg ctgcgtgttc caaagtgccc tcttggctag cctacctgtc cgacgacacg
     3961 gttacttggg aaaaccttgg gtcgacggtc ctgcggtgtg acacgccacg cactgatagg
     4021 catccaggta gctgcggttt ttgtagtagc tcgggtcggg cagctcggaa ccttggccgg
     4081 cgcccatccg cgacgccgcc gcatcgatca gcagcttgag ccgagccgat cgctcgggca
     4141 aacgcgaccg gacgggaaaa tgcgacaccg ccgggtccat cgccgcttgg ctcgcagccg
     4201 aaagaaactg atcggccact ttgaaacgca tcgccgcttc gggcaccagc gttcctgcct
     4261 gcgccgggtt taggtcggca cgctgaagct gtgactcgcc gcgcttggcc agcagatcgg
     4321 ccagcaacag cggccccatc acttcgcgcg tgagcgtccg ttcgcccgtt tttggcagag
     4381 gctgcggccc aagcgagcag ttaaaccgcg atgtgccaca ggccacgctg atcagctgct
     4441 cgacgtgagg tgcgtagcca gggtgaaggt tgggatcgcg gaccaacatc atcgccgtga
     4501 acgtgtcaaa ccagctctgg ctcgccgctg aatttatccc gagcagcaca tcggcggtgt
     4561 acatgccgtc gatggtctgg agagtaaacc cgcacgagcg gatcacactg gcgattcgat
     4621 cgtcggtcgg atcgagatcc gccgcccgtc gcagccattc gagcgctttg ttcatccgcg
     4681 cacgctgttc gtcctggtca tcgcctttcg agtagcgcgg tggacgggtc agctgttcca
     4741 gtttgaggta gccatcttcg acggcgtgct gaccagccac gagcgcaaac gcggcataga
     4801 gcagtgcccg cgccgcgttt ttctgatcga cttcctcgac gatttcgccg ttgtcgagct
     4861 ttgtcagcgt gtctttggcg atctggtccc agcgtcgctc gaaatcggga aacgccgcgc
     4921 gcgtgtcatc ggaaagcagg ttcagacctt ggaaaaaacg cggcttgtcg gtggcgaagc
     4981 tcgggggcgc atcgtcgccc tttccgcaag ccgacaaaag tagcagcgac ggcagcacaa
     5041 cacgaaagag cgacatcggc ggtgaactcc tgacaaaaca agtgggcgaa agcgcaaacg
     5101 cggcccgaac ttgcgacagg aaccacagca aaatcaagcg cctcgaaaac cgaggcagtg
     5161 cgagagaagg gagtcgaacc cttaagcctt tcggcgccag atcctaagtc tggtgcgtct
     5221 gccagttccg ccactctcgc gcgaggacag tgtaactgcc ctgtcgttca ccagaaaatc
     5281 ggcaaaaaaa aagccgtagt caaggactac ggccaaattt ttgtgagccc agtaggaatc
     5341 gaacctacaa caaacggatt aagagtccgc tgctctgcca gttgagctat gggcccgtgc
     5401 aagaagtgcg tatgtattgc gataaaaggg cggcccctgt caaccacaaa tctttacaag
     5461 gtccgctctt ttttgcactt ccgtcggaaa ctacagcttg tagaagtaga cgacgtccgc
     5521 tccggtatcg ggaaccgcga ggactttgcc caccggatcc cagccgatgt cggtcgcggt
     5581 cgtggcgcca tcctgggcga cgttgcgcag gatcgtctgt gcaccggtgc tcggatcgac
     5641 gctgtagaca atcttgtcgc cgggcgaagc gatcatgtac ttgtttgcga cggtgtcttt
     5701 ttcgatgccc tggtacgcga gcgagttctt ggtgctgttg acgagccggg tgcggccaga
     5761 gccatcgaga ttgcaggtgc tgatgccgcc cgattgcatg atgacctggt tgataccagc
     5821 ctcggcaatt accaccttgg tgccatcgat atagacgccg cttgtgccag caaaggtatc
     5881 agcggcaacg atggtggttg gattggcccc ggctgcaaac ggcgccgtga acttgagcag
     5941 gcgtcgaccc gtggcatcgg cgacgtaggc gttgttgctc gaatcaagcg cgacgtcgat
     6001 cataaacgtc ggatagccag ccagcgacgc gagaccgccc gccgccaccg ttgcgctttg
     6061 caaaggcgcc accgtcgtcg ggttggccat gtcgatcgcc cacagcttgt cgatgttgcc
     6121 gacgtacagc ttgccgccac cgccgcgcag accaaacggc gcattgagcc gcggcgtcgc
     6181 cgtgttggga taccaagcgt gattcgggtt catcaggttg gccggcacct tcgagatgaa
     6241 cgccgtcatg ttggtcgaaa acgtcttggg gtccgtcacc gtggcgccac cgacgttcga
     6301 tacataccaa gagctggtgg cggcgtccca gaacgccgac gtcggctgct tgaagccggt
     6361 cactttcgta ggagcagcca cccccgcaag gtcaggattg gtcagcgcca tgtcaccgct
     6421 gcccgtggcc atgtccgtcg aacccaccgc catgtcagcg gtgtcagtgg gcgtgtttcc
     6481 catcgggtcg ttgttgcagt tgaaaagcgc caccgatgca atcaccgacg cagccagacc
     6541 aagcgaagcg aacgtaagtt tgttcatgga caggactcct tgagggaaag agacgacaag
     6601 tccgcgggga ctcgacgata gggacgcatt ataccttttg cgtgaagcgg tcttcaggca
     6661 tttgcgaagc agagaccctc gcttgccaac ccccccgctc gccaagctgt gctcaaatgg
     6721 gagcatttcg tcgggggctc gttaccatgg caaacacatc gctgcgcatt tccccccgcg
     6781 tccacctgtg gctgctgtgc ggaacggtcc tttcggcgct gtttggtttt gtctatgcgc
     6841 ggctcgtctg tcagtacatc tgcgcgctgc cgacagatcg tctggcccgc aacatcgcga
     6901 ttgtgtcgct ggcccagttt gcgctgcgcc accatctgct gacgcgaccc attccttggc
     6961 aatcgacccg cacgaccgct cgcacgttgt tcctgcggtc tgtgctgtcg tggttcgtcg
     7021 ctggtgcgct ggcgatcggg cttcactttg cgctgtatcc agactttccg gtgggcagcc
     7081 acttcaagtt tgcgctggcg tattgggcgc tcggtggtgg actgtgtacc caactggaat
     7141 acctgctgtt tgaacaggag ttcccggccc aggcggtcga cggtgcaaac ccgctgcgcg
     7201 agcgactgcg cgatcgtctg atcgaaggct atgcgatcta tacgctgaca ccggcactgg
     7261 tgctgctttt gacactgctg cgcgtggtcc gcgagctggg tggcacgacg cagcaggtca
     7321 tcgaatccgc tctgctgatg ggggtctttg ccgggatggc tctgcttgcg gcgctgctgt
     7381 tcggacgtag cttgcgcaaa gacagcgaac gactgatgga agcggtgcgc aaagtgggcc
     7441 aaggagactt ttccccgcgt gccaaaacca cccgtgccga tgagctgtct caggtcgccg
     7501 tcggcatcaa tgaaatggca agcggtctgc ttctgcgcga gcgcattcgc gacgctttcg
     7561 ggcgcttcgt ttcgccgcag gtggcgactg agtttatcga aacctacgcc aagtctggtc
     7621 gcagcgccga gctgggaggc aaacgccgcg acgtggtcat cctgtttagc gatctgcgcg
     7681 actttacccc gctgtcggaa tcgctcccgc ccgagcggct gatcgaagtg ctaaatggct
     7741 attttgcgga aatggtcgcg gcaatacatc agcagcacgg catcgtcgac aagttcatcg
     7801 gcgatgcggt gctggcggtg ttcggcttgg ttgactcccc gacgggtcag gcaggcgaaa
     7861 atcccgcgat ttcggcggta cgggcggcgc agacaatgca gcgacagctt cggttgtata
     7921 acgacacgct tcggcagcaa ggcatccgct tgtcggcggg aatcggcatt catgccggtg
     7981 aagtgatcgc gggctatttg ggcacgcacg aaaggatgga atacacggtc atcggtcaca
     8041 acgtgaacgt cgccgctcga ttggaaagca aagcccgcga gccgctgccg acgctgctgt
     8101 ttagcgatga agtggcgcga cggatcgggc acacgctgcc ggtccgtgcc gcaggggaag
     8161 tacagctcaa aggggtcacg agtgcgatgc cggtctttac cgttgatgaa ccgccaacgc
     8221 tccccattgg atcgagcggt cagtagtacg gacggggcgg cggtggtggc ggtggtggcg
     8281 gcggcgaaaa gcgaccggga aatggatcgg cggattctct ccggcgggct tcgtcatcga
     8341 gatcgggacc tacggctggg gcgggcgcgc cacgctcgcc gaacagcgca attccgatca
     8401 caccgacgtt gcgatcacca aattccgacg tgcgggcggc ataggagttg cgcaccgatc
     8461 cgaaacgaaa ggccgcaacg tcggtcatgc tggtacggaa accgtcgatg cggacgcgat
     8521 gaaaggcgtc gacgacatac ccacgctttt cgaggctggc tgagcgacca tcgacgacat
     8581 cttggccatc gacggaagcg accatttcaa agcgctgacc agtgttgttt tcgaccacga
     8641 ttgaatagtg ctgccccgcc gatccgacga cgagaacgtg gctctgaagg tgataggccg
     8701 gcagcggatt gccgttgcca tccacaaccg agaccgtcac accggaccat ggaatcggac
     8761 gattgccgac gtagctgcgc cagatcggct cccccggata gtggcgacgg agcagtctct
     8821 cgcgctgggc ttcgacgccg cgcgcatcgt tgtagtggat cgtgccagtg aacagcggcg
     8881 acgagctgcc gcgctcaaac ggaacatctc gcacgggtga atagcgcgac tcgccaaact
     8941 cggtgccgag ccctggccgc tcgacctgcc gaggagccac cgcttcacgg cgactttttt
     9001 cgggcagagc caatgagggc gaagacggcg tcggcgcccc accgtagcca ccgtaggctt
     9061 catccgcaga cccgcccgtg ccactttgca tttccgactg tggataaccc gatgtgtctt
     9121 gcccgtatgg ggtcgcgtaa tcgggtcgca cgttctggct gctttggtgg cagccgagca
     9181 gcatcatcga cgaaaaaacg ctcgtacaga ccagcttggt tagccaagca gtaagccgca
     9241 tcgtcaatcc tccctcccaa gttgtcgagc tactaacgca atcgttcccc atgccttaca
     9301 cggtctgata gatttttttg gtgccgtcgg aaacgccagc gccggacctt tgtgcagacc
     9361 tgcgttctgc gcttttgtcg accgatctgg ccaagctgga agcgctgtgg caaacgatcc
     9421 tgaacagccc accgacggaa aaacgacggg ccgcggcgat tttggccaag catggtctgt
     9481 ccgatcgcgc cgcacagctt cacgagcagc ttggggatct gccttccgcc caagcgctgt
     9541 atcagcaggc gggccttctc accgacgccg gacgggtttc agaacagctt ggcgacaagc
     9601 gtttggccga agcactctac cgtcagagca tcgtcgaaca tcgcgcagat accgcggcgc
     9661 agtctccgga tggggcggtg gccgaccgat cgtcacccgc gctgcggctt ggccagctcc
     9721 tacttcgcca aggccgtgcc gaggaagcca tccctttttt gcaggcgatc cgacgcaagc
     9781 tgctgacgaa caacacgccg ttgccgctcc tcgatgaagt cgagcccgcg ctgctgcatg
     9841 cacttgtgca ggttcaactg ccggaagcgg cgctgtcgct gcttcagtcg tattcacgcc
     9901 gtcacccgga cgcgccaccg acggttgccg agtggcttct gaccgcgcca cagcccagtg
     9961 tgcaagaacg aatgctcctc ggtcgatatc gcttgcagag gctgatcggg gccggggcga
    10021 tgggtcgggt gtttgtcgcc gaagacacgg ccagcagtcg gtccgtcgcg atcaagctgc
    10081 tgccaatgcc gatcgattcg caggcgcgcg acagcaaatg gcagatgttc tgtcgagaag
    10141 cgcagattct gcagacgctg cgccatccca acatcgtcgc ggtgcacgac ttttccgaat
    10201 cggcgggggt gctggtgatg gaacttttgc gcggtggatc gcttggggaa cagccgctgc
    10261 cactgtcgct ggtcaagacc aaacgggtgc tgcgcgatgt ctgcgccgga ctttcagccg
    10321 cccatgccgc gtctgtgctg catcgcgaca tcaagccgca caacctgttt ctcgacgaag
    10381 cgcacaacac caaaatcggg gatttcggcg cggcgctgct cgcccagctt ggtgcgacgc
    10441 agaccgaaag cctggtcggt acgctcgctt atatgtcgcc cgagcagcta gaaggacaaa
    10501 aactcaccgt cgcaagcgat ctgtattcac tcggggtgac actgtttcag cttctcaccg
    10561 ggaggcttcc gtttgtcggt cccgattggg taagccagca tcttcggcag ccgccgcccg
    10621 atccgcgatc attgcgtccg gcgctgcccg cagcgtgggc cgatctgtgc ttgcagctcc
    10681 tggccaaatc agaagccgac agaccgtcga cgatggacga actggcgcag cggatcacgc
    10741 agcttcccga cgcagaaact gcgcagcaag ccggcacgct ggaaggaaaa ctcccgtcgg
    10801 cagacgacgg acttttgccc cacttggaaa cccacagcct gcacccgatc gatggcacaa
    10861 gtccgacgct gctgtatcag accgggcaca gcgcggtgta tcacgtcgtt tcggccaagc
    10921 tagccagagg cgtactcatc gaacgatttg atcgcgaaaa actgatgacg ccgacggggc
    10981 aacatcatct gcgctggctg cgcgccatgg ctcggcttgg tggaccgggt ctacagcgcg
    11041 tgctgcgcat catgctcgac ggacattcgc cagaagttca cttcgaggaa accgtcggct
    11101 taccgcttgg cgcaaacggc ccgccctccc gccaagatga agcgatcctg ctggcgacgc
    11161 tgcacgcgat ccacgccgaa gatgaagttc acggcaacat cagcgacgca gtgatcgtcg
    11221 aaaaatacgg ggcccagctg caagtggtgg gacgcggtcc gctcgggtgg aaaagtccag
    11281 cgccgacgcc caaagacgaa gaacggacgc ttttggccat gctgcgcagt cggaaaacgt
    11341 agcgatcacc gacgcaggag cacgtagacg gcccctgccc cgccatcggt cggacgcgcc
    11401 gtcgcaaagc agagcacgct tttggcaatc cgcccgcgct gaagccacag ccggaccgca
    11461 tcttttagca ccggaatttg gtctttcgaa ttgagcccgc gaccatgcac gatcaggatg
    11521 cagcgacggc catttagctt cgagtcatgc acaaacgttt cgacccgcag ccgcgcttct
    11581 tcgcggttta gtccgtgcag atcgacgtgc gcctgcactg caaaatcccc ccgtcgcagc
    11641 cgataaagaa gtctcctgtc gagcccgtgc gcgatgcctt cgatgtattc gtcggtgtcg
    11701 ctgatatcaa acgaaccgct gcccgacacc aaatcggata gctgcgcata cgcttcggcg
    11761 tcttccgaca gacgttgccg tgccgcacgc tcttgctcga cgggacgctc gcggccttgg
    11821 acagccagac ggtttgtgcc ctgcatttcg cgccggaaga gttcttcgtc ggataactgt
    11881 tcctcttcgc tggtcgtcag cttcggcggc ggcggtgctt tgggtggttc tttgaccgga
    11941 gctttgtcga gaagccccca caactctttg cgcgctgcca aaaacggact gttgagcggc
    12001 ggctctttcg ggggactcgc actggttttg ctgcgcttct tcagttgcgc catgactcac
    12061 tcgtacctgc gaccggcaat cgctgcaagc ttgctgctga ttcgatgtcg cccggcagcg
    12121 tatactttcc accatgccac acgagatggt cgatagatcc ccgccctact gtcggatttg
    12181 tgaaggtgtc gaaggcacgt cgctgccaac ggactgtccc ggcgaaaagc tcacgtcggc
    12241 gcaggtatcc ggcgtccact tcaagacgct cgattaccga gacggtcgct gggatgaccc
    12301 cgccgtgttc gactggaagc ccgccgcgat gccaacggcg tcaaacttcg gcggccccag
    12361 catcgtcgtc accggcgatg atggttcgac gacggtgagc ggcagcggcg gatcgcgcca
    12421 catcaccacc atcattccaa ctgagcccgt ggatgaaccg gaagaaaatg gcggcgctcc
    12481 atcgctgatt ccggtgccaa tccccatccc gattcccatt cccatcccga ttcccaccgg
    12541 cggtagctct ggcccagccg aagaggaaga aggcgatccg agctacgacg tccgcgtcaa
    12601 caagtagcca catcgtccct aaaacatcgc ccgtcgcagc gctgaaagcg gcgcatcatc
    12661 gcgcagtcga accgagtcat caccgacggg ttcaatgccc agcagcgtca gtgtttcccg
    12721 cagtggcagg gcgtttcgtt cgcgcaaata gcgccgaacc agcgccggtg aactggcctg
    12781 cccaagccct tccagcaccg attcttcatc gagtcgcgtg tgcaaactct gcttacgaag
    12841 cgaccgcatc acatcatcga gcgaccgttt gccctggctc tttttgcgaa tctcgacatc
    12901 gagcaaaaac gccagacacg cgccgcccca gtacacccgc gtgtagcgat aggtcttgtg
    12961 caagttggcg ctctccgttt ccagcgacgt cccgttcggc tggcagtgcc ggcgaaaacc
    13021 atcccacaaa tccgaccacg cttccttcgc cgtcagcgcc ccaagccgcg ctcgcaagat
    13081 atcttggtaa tacgtggtaa aaccctcaac aaaccacggc accttgtagc gcatgagcgg
    13141 attgccgata tgaaatatct cgtgcgttgc cacccagtcg tctgaaagcg gaaaccgcgt
    13201 cgcatccgcg ccaaagtaga tcagcaccga cgggcgcagt ggccgaagca ccgtcccaaa
    13261 cgagcccagc tcagtctgtc ccggccatgc gacgatcacg atgtgaacgt tcgacggcat
    13321 ctgtggaccg tatagccgct cactcgcccg cgaggcctgc tcgatccacg tccgcagttc
    13381 cagatcggac gctcggcttt tcccgaccag gtgcagctcg acctgcgaat gcgcaagcgc
    13441 gagcgtccca agcggacgaa cagcgccaaa ggtcacgtag ctgccgccgt cgtactgcgc
    13501 cgcatccagc tgatagcgca agcccggtcc gggcaacttc tgccaaggca ccgtcagcgc
    13561 cgtcccggtt ggaagctcga attcaacatc gatggatggc gacggcagcg acggagaatc
    13621 cggaagtggc agcgggtccg ggcgcagcag caccgcttcc tcactgaacg tcatcgcctg
    13681 ttcgtggcgc tgcaccaggt ccgcgttgtt gaaatgatcc gccgccgccg caagatcgta
    13741 cttgtagcgc agccgcaccg ggaccgcttt tgcgtcgatc agccggaagc agtccccatc
    13801 acgcacaaga ggcagcgcgc gtccgtcgga aaatatctgc tggatatcgc gcacaaaccg
    13861 ccccgcggaa ggcatgtcgg cgcagacttg cggtgtcgtc ggtgcgggaa acgtcaagct
    13921 cacgtttgcc tgcgcatgcg gcccatccgt cagcttgacc aagcaatgcc agccacgcgg
    13981 cagcgcagac ggcggctccg ccccacccaa aagcagggcc gccaccgcca aaagcgaaaa
    14041 acgaagccaa tgcacgcgct acatacgccg ccagtgaccc ggaacatagc gccagccgcc
    14101 cggagcttgc acccactgcg cgcgctgcca gccaaagcca tgccgacgcg cgacaaagtg
    14161 ccccggcgtc cagatccagc gcacgccatt ccacgtccag tgaccgccta cccattcatg
    14221 ctgcacggac ggcgccatcg gcacgacttc ttccatcggc ggcggcggtg cgctttggac
    14281 gattacgcca cttggtggcg gcggcccgtc catgtactgc ggctgacgga gcggttccca
    14341 gtgaccgggc gcaaacaccc actgatgtcc gacgcggacc cagtgcgccc gaacccacac
    14401 atggtgaggc cgcgccatgt cccaataacc cggcacccag acgtagcggc ccattcgcca
    14461 ggcccagtga ccagggatcc atgcgtgccg ctgagacggc gccaccgggc gaacttccat
    14521 ttgcggcgcc ggtggtgcca tctcgacccg aacttcgggc tcggccacgt atcccggctg
    14581 cggcgcgtac tgcggctctg gtgcgtattg cggctgcggt gcgtattgcg gctgcggtgc
    14641 gtattgcggc tgcggtgcgt attgcggcgc tggctgcggc tgggcaggaa cgtactgcgg
    14701 agctggctgc gcgaccggct gcggcggcac atagggtggc gcaggcctcg tcggatactg
    14761 cgcaagcgcg ctcatcgaag aggcgaacag aagggccatc gaagcaccga agatcgtgcg
    14821 aaagcgagcc atggagcgat cctttccctc gacaagcaga ccggcgaaga cgtcgctgcc
    14881 caagtgcggt cgaggctttc cgcattttac ctaacctcgg gcctgttcgg ttagacgaag
    14941 gggcagccat gtttcttacc cgaaaatacc tttctgtcgg cccgaccgag aagctgctgg
    15001 ggcgaaccac aagcgccgat gcgaagacga gttcacgttc gacgtgccgt aggagttgac
    15061 cgctcaagaa gggcgcgcgt atgctgcgga cgtttttaaa gggaccaagg aatgcggctc
    15121 tattcgggaa agattccttc catcgcacag gacttgattc gcaagctcaa ggaagatggc
    15181 gatattgagg tcagcgacgt ttcggaggcc cagctcgacg tggaagcggt gctcaaagag
    15241 tacctgcggc tcgagcgtga gctgacggag aaggccaagg actacatgga aaaacggcgt
    15301 ctgccctacg agcagctgcc gaaaatcaag cgtgccatgg ccgaagagcg cgacatcgga
    15361 atcggcgacg agtcggtgtc ctacatcgcc aaccaaatcc tcgaagcgtt tatgcactcg
    15421 cgctttgtcg aggaggtgtt tgccgacgat gccgacatgc ggaagaaaat ccaaggcatc
    15481 ttgcgcaagc acatgcaggt cgacgccgat atcgatcacg aagtgcgtcg acgcatcaag
    15541 aatctgcaag aaggcacggc gacctgggaa atcgaatacc agaaagtcat ggagcagctc
    15601 cgtcgccaac acaagctcga ataagagcgc acgcgcccca caagcaagtc agttacaccc
    15661 cgcaggagaa agcatttgag tcgagaagat ctggtcgaaa tgaacggaac ggtcaccaac
    15721 gtgatggtcg gcggtaactt tgaagtcgtc acccccgaag ggcacaagat cctggcccgg
    15781 ctgtcgggtc ggatgcggcg cttccgaatt cgcgtggtgc tcggcgatca ggtcctggtc
    15841 ggcgtctctc catacgatcc gacgcgcggc ctgattacgc gccgtaccta gcaccacgtc
    15901 ggcatctaac tgcttttgcg tctgttggta gcgaaaaagg gggttattcc gtgctcaaag
    15961 ttggcgacac cgcaccggat ttttcagcga cagcgacgac cggcaagccc gtcaagctct
    16021 cggagctgcg tggcaagcgg gtggtgatgt tcttctttcc gaaggcgatg accaccggct
    16081 gcaccatcga gacgcggctg tttcgcgacc actacaccga gatcgctgcg ctcggagccg
    16141 aagtcattgg cgtttcggtc gattccaatg aaaagcagtg cgagtttgcc agtcgcgaag
    16201 gcgtgtcctt cccgatgatc ggcgactcac aaaagacgat cggcaagagc tacgacgtga
    16261 tgtggccgct gctcggcgtc acccagcggg tcacctacgt cgtcggtccc gatggacgga
    16321 tcgaggcggt gttccatcac gagctgatgg tgggtcggca cctcgaagac gtaaagcgcc
    16381 acctgcaaag caaagcggcg taacaaacgc tggcgtccaa cggaaactcg cccggcgacg
    16441 acacaaccgg cgtcgcacat aaaatgtgga ttattcctcg gggcgggagc gcaggcgggc
    16501 caggtaatac aggttcagca gcacaaacag cccaagcagc gcggcaactg gcggagccca
    16561 cttgacctgc tgcggcgtct ctccgtcaaa cagcacaaac gcatcttcgg gaatggtcag
    16621 cggctcgctg acttgcacgg cttggacttc gctccacaga agcagcgtgc tcggctcgcg
    16681 aaccgcggtc aacagcttgc tatcaacagc cgccgccggt ggagcaggag ccgaagttgc
    16741 cgtcgcagcg ggagctgccg ccggagttgg ctggggcgcc actgtcggat tcgcaggcgc
    16801 agtcgggatc gtcggtgcgg caggagaacc aggcgccgcc ggagctgggg caaccgccgc
    16861 ggtccgatag cgaaccggct gctgaagcgc atcgggacca ggaatctcga tgcccgtggg
    16921 accaagctgg agaagcgcgc tcggaacccg gtaggtctcg atgcggtgcc acagccagat
    16981 tcccatcgag tcaatctgcg cgagcatctt ctggcggtcc ggacccttgg ccggcagacg
    17041 caggacgtaa ccaaagccat cccgcgtttc gatccccgga ccgtggggca ggcccagccg
    17101 ctcgacttcg tagcgagcat cgggttccga cgggaacttt tctttcgaga gcagcacccg
    17161 cacatcgtct tcgacgagca catcaatcag aagatcctgc tctcggctca ccgatagcgg
    17221 ctgtccagca cgatcgagcg tcgcatacgg cagcgtcagc ttgcccgctg gtgccgactt
    17281 cagcttgtcg aggtcaatcg cccgcagcgc ttgggtctgt ttgaaatacg tgcgcagcgc
    17341 atcgacaaac cagacatcgg aaatcggacg cagccgcccg acaaactcgt tggccgcatc
    17401 tgccgacagc gatgctttgc gcgccaccag cagtcgattc gccgacccga gcagacggaa
    17461 cacctgcacg cgacctcgcc cgcctttggg gtcatacgac accgagttgc ggtgatcggg
    17521 cagtcctgac agcttgacga acttgtcgcc ctgacctgcc agcttgcccg tcgcggcgcc
    17581 ctccggtgtg ccgacatcgg tcggttgctc gggcaaaagc gcataggtga tgtcgccacg
    17641 catcttataa agaagcagca gagacaccac catcacggcg attgcgacga gaggatgacg
    17701 acgagccggc ggcaccttga ggtttaccag ctcgggatcg atggcatcgg gggaaatcgg
    17761 cgcgccgggt tcagccgaag tcaccagaac aggttcggca ggttgcggcg gcgctaaatt
    17821 tggagaatcg gaacttggtt ttaaagccat gcttcaccga cagaagtgag cgcatcggcg
    17881 acgttatccg agcccggcga gaattgccat tgtttttggg ggagccaggc cgcgccgcgc
    17941 gttttcacgg ttcacggcca ctggggcggg ggaaaagcga agaagcgacc acagagatgt
    18001 ggtcaccgac gagactactc ggcgagcgcg cggtcgattt cggtcttgaa cttctcgaag
    18061 ggctgcgcgc cgctcaggaa gacgccgttg acgaagaacg ccggggtgcc cttgacgccc
    18121 gcctcgctgc cttccttcat gttcttctgc actgccgcgg cgtgcttgct ggtggtcaga
    18181 cactcatcga acttggtgcc gtcgagaccg agcttgcggg ctgcgtcctt gagcgccgcc
    18241 tcgtcgagcg tgctctggtt gtcgaacatc cagtcgtgca tttcccagaa cttgccttga
    18301 tcgttggcgc actgacccgc ctcggcagcc ttctgcgctt tgtcgtggaa gctgagcggg
    18361 aagtcacgga agacgacgcg gaccttgttg ccgtacttgg cgatgacttc ttccatgacc
    18421 ttgcggcccc gaccgcagta cgggcactgg aagtcggaaa actcgacgat ggtgactttg
    18481 gcgtctttgg gacccttggc cggaccttcg gtcgcgacct ggactttggg ggccgtcagc
    18541 aggactttga caccggcctt ggacttgatc tcttcgatca cgtcaccgac ggcgcgctgg
    18601 cggttctgac ccgtcagacc cttgaccagc atgtctttta cctgctcaaa cggctgacca
    18661 ccaatctgcg gcggcagacg ggctttgttt tcttcgtaga acttgcgcgc ttcgtcgacc
    18721 gacacttccg gaacgcgctt ttcgacctgc tgcttgagga acgcatcctc gtcgagaccg
    18781 accttcttgg cttccgcctt gatgacgcgc tcgttgatga tgttgtcgag tgcgcgctcg
    18841 cgaacctcga aaagctcacg gcctgcggct ttgtccagct cggccatggt gactttctgg
    18901 ctgccaatca tcgcgacgac ggtatcgccc gacatcgatg acgaggccag gttgctggga
    18961 gcattcttcg cgcaggccgg agcgaggagc agcagggaca gggcaacgtg cgtaaggttc
    19021 atcggatggt ccttttaacg tcctttgggt cgtggttatc ggcgagcttt gctacactgc
    19081 gaggcatgtc catcctcagt cggttgggga acctgattaa gtcgaacctc aactctgccg
    19141 tcgataagtt gtctgatccg gcgaaggaaa ttgaccttct cattcaggaa atggaagacg
    19201 agctttcgca gttgcgcgtc actttacgcg accaactcgt gcaagagaag ctcgcacaga
    19261 aaaaagtcga tgaggaatac cgcaacgtcc aaaagtggca agaccatgcc gagcgggcgg
    19321 tgcgggcagg cgatgacgag ctggccaaag aagcgctgcg acgacgggtc gaagccgaag
    19381 ccaagcttga agctgcggaa cggacccacg ccgagcagtc gcgtcacgtt caaaagctat
    19441 cggacgatct gaaaagcggc gatcgaaagc tgaccgagat caagtcgcgc aaagaatcgc
    19501 tcaagtcgcg ggcgcgggcc gccaaacagg ccaatgatcc agaaggtacc gcgatggatc
    19561 gattcaatcg cttggtcgcc gaaatcgaaa cccaggaaga acaagcggcg gcgatggccg
    19621 agcttgtgcc ggagctgggt tcgcagaggc agaacgaccg cgatcgcgaa accgaggagc
    19681 gcttcaatcg actgctcggg ccgggcaaag acagcaaaga gagcgacatc gattcccgac
    19741 tcgccgcgct caaagccaag cagcttcccg ccaaatccga cagctaagcg cgcttttgcc
    19801 actcgctgcg cgtcaccacc ggaagctgca agcagcgctc gcgttccaga tccaggtttc
    19861 ccagatgcag cgacagcggc gcctgcaccc acttgtaaat cgcccgcgca aacagcatca
    19921 caaacttgtt ggcaaagccg cgcagacggt ccgccgggta ttccggaaac atgtcaaaga
    19981 gcacgtcggc cagctctttg gcatcgagct tttccgccgc gtgcagcgca atgcaggcat
    20041 ctagcaccgg gaacggcatc agatcgcgct catcctgctg atccggagcc aattccgcag
    20101 tggccggaat cgcgatggtc tggcgaatcc cgtcgagctt ttcggtttcg agcagataat
    20161 cgagcagata atttacgacc gtctttggca ggttcgcaat caccgccaga cagcccatca
    20221 ggtcgccacc aatcgttgta tagcccgctg ctttttcgct catgttgcca gtctgaagga
    20281 acagacctcc cacggcgttc gaccagttcc acatccgctc ggcgcgcagc cgcgcctgga
    20341 tgttctgcac cgtaagctgg gtcagttctt cgcccggatg cagcatcttt tgggtcgctt
    20401 cgacttcgcg cgcaaacgca tcttcgatcg acagaatcac aaaaggaatc ccaagctccg
    20461 tcgctgcgat ctctgcggca ctgcacgtag cggaagagga atagcgcgtc ggcatgtaaa
    20521 aggcgcgcag catgtctgcc accttctgtt tgacgaccgc ttccccttgg gacagatggc
    20581 gtcgcgtaac atagcgatgc gcaatcagca gacagagcag gctgtcgcga ccaccggaaa
    20641 gcgccacgcc gatctgtttg aacgcgccag tcttttcaaa gtaatcgccg acgcccattg
    20701 ccatcgcatc aagcaattcc tcacagaatg tgcggcgcgg atcggcaggg acagaaggag
    20761 aaggcaagaa gaagttgcga tggcttggga agggataaca gagcttgctc gcagagccaa
    20821 tcgcaccgac ggaaatgacc tgcgcgccac aggtcgagga atcgcgccgc acgccacttc
    20881 cgctctgggc caaagtacgt gaaaggaatt cctcgcgatc catccgccag gttgtacttt
    20941 cggtgcgcag ccgcgtcgtc cgccgcagat cgaccgtaca gaccgaacca ccttcgacaa
    21001 aacgcggcgc ttcatgcagg aagcggccat tttggcagac aaagccaccc ccatcgaaaa
    21061 tcagtccgtc gttcgctcct actgcattca catagacgac cgtgacgttg ttgtcacttg
    21121 cgcgcgttgc gaccatttct cgacgggttg actgcacacc aatgcgaaac ggagacgccg
    21181 acagatttac gataagctcc gccccttggt agctgcgccg ccgcatcgga ccgtcgggag
    21241 accagatgtc ttcacagact tccagcccta cccttccgaa gtcgcagtca aacagcaaat
    21301 cgccaaacgg aacctgtcca cacggtccca gatcaatctc tgcatacaga ccggggaagc
    21361 cgcgcgcgag cgtccgcgct tcgtaaaaca cattgtaaag tggcagcttt tccttgggaa
    21421 cgacaccaaa cactttccct tggtaaagga atgcggcgca gttatacacc tgctcaagtg
    21481 actgtacgag cacaccacag acgatcgcca tcggcacctg tgccgtcgcc tgcgccagct
    21541 tgaaaagacc ttccgcttgc gcagaaacaa agcctggcca aagcagcaga tcctctggag
    21601 gatagccgcc aatcacctgc tctggcaggg ctgcaattgt cacttgctga tcggcaagct
    21661 tgtgacaaat tccgatcgcg cgttccacat ttgcatcggt tgccccgacg gtggtgttga
    21721 cattcccaag acccagccga agtagtctca tgcgatctcc gcgtgtctgc ccaaaagaaa
    21781 agcaaccatt gtgcttgttc tacatcgtcg acagcagttc gtctatcaca tgcaccagcc
    21841 gattcgatag tttgataggc caaggtgctt ccgcagaacc cgacgtcgcg acttccagtt
    21901 tgcgcagatg cgccggcaga gcagtacgct gcgctgagac atgatcgcgg gtttccgtga
    21961 gcgaaggaag cggtgacacc agctttttgc ctcgccacag cggctgaagg aggggcgttc
    22021 cgcccggtct tgctggttca tcatgaagcg tcaacagatc atgatctggg aaacggtaga
    22081 cctgcaaacg gcccggcagt gtggcctttc cggaaccgcg cgcaagcttc atcgtcagct
    22141 ttccagtcgc attttggaca agcttcccaa cagctcctat tcccgtcgct gcgtctggac
    22201 tacaggagat gttttcaccc actccgaaac catcgccgcc ggctgttgcc aaatcgcgaa
    22261 cgcggtattc atccagacca tctgacaaaa acagcttggc gtgtggcgca cccagatcag
    22321 aaagcagctt gcgggctctg cgaacagact ccggagtcac cccggaatcg agccgcactc
    22381 cggccagttt tccgtcggta gcgcgcactg cattttcaat gccgcggaac gtatcatagg
    22441 tatcaaccaa cagcgtggca tggccgggaa atagttcaca gaaacggcga aaaaaatgca
    22501 tctctgtttc cgcaaccgga gtctgcaacc gttcacaagc ttgcaccgca aagtgatcca
    22561 tggttcccac cgaaggaatc ccatgacagt acgtcgccgc caggttgctc gtcgctgaac
    22621 agcccgcaat ataggcagca taagcggcat cgatcgcagc catcggatgg gtcctccgct
    22681 gaccaaaatc aatcaccgat cgcaggactc cgtcatgttg ggcagccgct accacacgcg
    22741 aagccttcga cgccaccatc gacatgtagt tgatgcgcga cagccacggc gtttcgatca
    22801 gcttgcacaa cgtgaggtcg gtgcgtacct gcaaaagggg cgtataggca aatagcggcg
    22861 ttccggacac gagcagcgga ctgccatctt cgcgctgtgc cggacccgca aacgccagcg
    22921 taccttccgg aatcgcgtcg atttcaccat caaaaccatc caatcgcgcc agcgcatcac
    22981 gaaccagctg ccccgttttg gttgaaagcg ccggacccag aatcggatgg gaatcgagca
    23041 gcgccagctc ctccgcagaa aaacgcagcg actgacaatg ctggacaatg ctgcgcagac
    23101 cacaacttac gacgtaattt cgctgcttgg gaagcttgcg gaaaaagaag ctcatcgcaa
    23161 gcggtccgtc ggaaagacga ccatctgcag catgcgcaat cagcgtcgtc agttgataaa
    23221 catcaagtgt cagcgcagac agactcatgg tgcaccacct tgcaagcggc tgcttctgcc
    23281 gtgcaacact ccggcagcgg aaaagcgcgc gctcgtctct tcctctttgt ccggaaacac
    23341 tgcgcgcgtc agatcatcga tcacaaccgc agaaaatcct tccgctatcg catcaagcgc
    23401 ggaccaaccc acacagaagt cacgcgccag accacagaca aacacccggc gaatccgccg
    23461 cgcatgtagc agcgcaccaa gcccagttgt atggcgtttt ccgtcgggac cgatgttttc
    23521 acgaaacgca ctgtaggaat caatgtgcgg ataggctccc ttgcgcaaaa tgacacccac
    23581 cgaatcaagt gggagtcctt gatgaagcgc agcgcctggc gtcccctgca cacagtgcac
    23641 cggccacagc gtctgatccg caccatacag cggaatcgag gaaaacggcg acagaccatg
    23701 ctgtgcggca aagctcacat ggtttttagg atgccagtcc tgcgtcgcca caacaaaatg
    23761 aaatcgcggc acaagcgcag cgatcggcaa cacaacctga tccccctctg cgacggcaag
    23821 tgggccaccc ggcataaagt ccggctgcaa atctaccacc agcagcgcgt catgctcccg
    23881 atcgattgtc cacacatcag attgggcccc atcggtcatg gtcgttcctt attgcttccg
    23941 cgagctttca caacacgctg cggtcagcct gaaatagaaa gatatccgat ttgccgcaaa
    24001 gtggtacgat ctcgacggtc ccgacgctca cttgcgggct cctgaatcgc ttttgccttg
    24061 tgcgatgaac ttcaattccc aattggcagg agagatgtag atgaaagacc ggaagcgact
    24121 tgtatggata tctgctgcgc tgctgactgt ctcgctctca acgcactgcg actctggaaa
    24181 cacgatggga aatccccctc cagatccgcc ccctgaaacg ctgaacctga ccgatgcatc
    24241 ccccaagctg atgaaaaacg gaggaggaac tgcggtcacg ctcaaaggaa ccgggttcaa
    24301 gacgggtgcc accgtcacaa ttggcggcgt tgccgcgcaa gatgttgtgg tggttgattc
    24361 ctcaacaatc cagctaaaaa gtccgctcaa agccgccacc tgcggcaccg tgaccgtcgt
    24421 ggtaaccaat cccgatggga atgccgcgat gcgcggtgat ctgatcacgt ttttgaccaa
    24481 ccgcgtgtcg tttggcgttc ccaatccgat cacgatggga acctatccgc gtcaagtcct
    24541 ggcggttgat ctgaacaaag atgggaaagt cgatctggtc tctgccaaca gcggtcaagc
    24601 agcccaagcg ctggtctatc ggctaggaaa tggcgatgga acgtttggga atccgacttc
    24661 ctacaccgca ggcagttcga catttggcgt cgctgctgtc gatctgaaca aggatgggaa
    24721 tctggacctc gtgaccgcaa acagcaacag caatgacatc tctgttcgcg tggccacagg
    24781 ggccggtaca tttcccacag ccagcaccca gtacggagcc ggtgccgata cccgtgcgat
    24841 tgccgcagcg gatctgaccg gggacggcgc tgcggatctg gttgccgtca actatggctc
    24901 cggaaacatc agcatttaca aaaacaacgg agacggaacc ttcgctgccc aaagcaactt
    24961 gtccgtcgga gccagcccac aacatgtcat tttgactgac ctcgacaaag acgggaagtt
    25021 ggatgtggtt acggcgaatg cgaatggcaa caacttgtcg gtgcggctag cgaacggaac
    25081 gccatcgatc tttggtgcgg cagcatctgt tgccgtggga tcgagtccgt ggtttgtgac
    25141 ggctgcggac tggaatggcg acgggaaaat ggatctcgcg gtcgtcaaca acggcatgac
    25201 tgccagtacc tacatggtgt cgattctgct tggcgatggg aagggaagct ttgcaacccc
    25261 ggtcaacatc gacatcggaa accaaaccgc accgcgcggc gcagtggcga cggatctgaa
    25321 caaagacgga atcatggatc tggtcgttgc gggctacgga agtaacacgc tcccagtgct
    25381 catcggcaaa ggggacggca cttttgaacc cgtgctttcc gtcactgggc cagcattccc
    25441 acaatcggta gatgtcgctg attttgacgg agacggaatg cctgatctgg tggccacttc
    25501 aagcagtacc ggttcccaga acgttgcgac gctgctaggc cagtgtaagt aaacaaaccg
    25561 accgaaggca gcgcgacaaa aatcccgcca cgcgcaaaaa aatcttctct tgggccacgg
    25621 tgctggcatc atgccgcgcc acatgattgc gtttacaaac gtcaccaaac agtacggtgg
    25681 acagattctt tttgtcgatg cgtcctttca gatcaatccc ggtgaacggg tcggtctggt
    25741 cggcccaaac ggagccggaa aaaccacgat tttccgtctg atcatgggtc aagaaaccgc
    25801 cgatgatggg agtgtcgaaa gaccgcgcaa gctgacgctt gggtatttca atcaggacat
    25861 ctcaggaatg cgcggacgca ccgttttgca agagacggtg gcaggggccg gggaagtgtc
    25921 gacgctgggc gaggaaatga aagtcctcga agaacagatg gcgcaaggct gcgacgatct
    25981 ggacgctgtg gtcgcacgct acggagaagt gcaagcgcgc tttatggaat tgggaggcta
    26041 cgacatcgat gcgcgtgccc ataccatttt ggcgggactt ggactgactc cgcaacagat
    26101 cgaagccgat gttgggaatc tgtccggtgg ttggaagatg cgcgtcgcgc tggcaaagat
    26161 cctgattctg cagcccgatg tcctgcttct cgacgagcca accaactatt tggacattga
    26221 gtcgatcctg tggctggaag acttcctgcg ttcctatcca ggttctgtgg tgatgaccag
    26281 tcacgatcgc gatgtcatga atcgcgtcgt caaaaagatc atcgagatcg atggcggaca
    26341 gattcgcagc tacaccggca actatgattt ctatgaacag gcccgtcagc aggaagctgc
    26401 gcgacgggaa gcagagtatg aacggcagca ggcgatgctg gcaaaggagc tgcgttttat
    26461 cgagcgattc cgtgcccaag ccgccaaggc cgcccaggta cagagtcggg tcaaaaagct
    26521 cgacaagatt gaacgggtgg aaccgccgcg tcggctgatc gagcgggagt ttgatttccg
    26581 caagccggcg cgatcagggg acgatgtgat tcgggtggcg gggctccgca aagcatacgg
    26641 agacaaagtg gtccaccgcg gcttggacct gctggtacgt cgcggcgagc gctgggccgt
    26701 gatgggtgaa aacggcgcag ggaagaccac gcttttgaaa atggtggctg gggtgctaaa
    26761 tccagatgcg ggcgaagtca ccgtcggtgc atcggtgacg ctgggctact ttgcgcaaca
    26821 ccagatggaa caactggatg gcaccgcaac ggtgattgac gaattgcaat cgcacgcgcc
    26881 cacaacagga atcggcgtgc tgcgcaatct ggccggtgcg ttcggttttc atggtgatga
    26941 cgttgaaaaa ccagtgcgtg tcctctccgg tggagagcgc tcacggctcg tgcttgcgaa
    27001 gctcctgttc gatgcgccga acctgctggt tctcgacgag ccgaccaacc atttggacat
    27061 cgcgaccaag cgggcgcttg tcaaagcgct gagcagctac caagggacgc tgctgtttgt
    27121 ttcgcacgat cgcgcatttc tgcgcgccat cgccacgcgc gtgcttgagc tgacatcaaa
    27181 cggtccgcac atctacggcg gcacgtatgc agagtatgtc agcgccaccg gacacgaagc
    27241 ccccggcatg cgaacaaact aaacgcggca gcgacggagt gtcgtagaac ggacagacgt
    27301 tacggacgct tcgccaatcc atcgagaagc ggccaagctt ggtccaaata actggtctgc
    27361 accggtcttg ctgaaaaatc agaatagtcg cgcaaatcgg tgccgcttgg gttgtgcggt
    27421 cgctggttaa tcagcgtcat gtgcagacac cacgaagcgc ccgatgcgca gcccgcagcg
    27481 tcggcaggaa ccacaatgga acccgcccgc ggcacaaagg aattcgccaa gctgatcatc
    27541 cccgcagcat tcggggacag cgttccgctt cgaccgtctt tgctgtatgc aaagaacgtc
    27601 cgatacaacc gattcactgc atcccacgta attccgccgc cgccatccag gttgaagtag
    27661 accgtcttgt cgaaagtctt tcccgtcgaa tcaaacttgc tcgtgtacaa aaaacccagc
    27721 agttcgtttg caacataaga accgctggaa tgcgacgcga tggtgatcag cttggtacga
    27781 ctcccaatct ttggcagaag gtgcgcaatc agcttggtgt tgccaatttc tcgtcgaaca
    27841 taggtcacat ccgcaggacc acgcaccgcg tacaggtgtc cgactccgag cgcggaaagc
    27901 cgggctgcgt acagtgcatc ggtccatgcc ttgctctgtg aatcagatac gttgtagccg
    27961 gcatagccaa taaagacatt ttcccccgcg ttgccggtgc cgcggtagtt tactccgacc
    28021 ccaatcgatt cccacaccga tgctgcggtg gtttcagaga cagtcccttc gctctctgtt
    28081 tccagcggcg tgaaaggtcc cccacagccc acagaaagca gctgcacagc gcccacaaaa
    28141 ccaagcagca gcgacgatcg agacagcaca gaacgaatgt gaggggacat catcggggga
    28201 actcctaacc aggtatgtag tagatcgggg acgcccaagc gcgctcctga atcgtcttgg
    28261 gaaccgacgc atccaagcac ggcgcgggtc gctgggctgc cggcagcgcg ttacacagaa
    28321 gtgtattcca gcgacagctt gggttttcta gcacacggac gtagtagtac gcaggctgtt
    28381 tgggatcaaa ctctggatcg cggaaactgc cacacagcag gtctgcgccc tcgccctttt
    28441 gaacgcagga agttgggtcc actgacgcgc catttgcacg gtttccagcg acgtcaaata
    28501 ccttggtgat ggggccctgt gcggtcagcg ttcctttgac aatctggatc acttgcagcg
    28561 gcgttcctgg ccgcgtccca tcgcccggat cgcgcagcgc ctggacgaca aagcgcggtg
    28621 cctgcgcagc cttgccagca aacgttccgc ccatcggaac accattttga tagccttttt
    28681 cgacgagccg cggatcagaa cagagtccat ccggcagatc gaaaccacca aagaaacgaa
    28741 ccgtgatgcg gggaccgctg gttccaaagg tctcgcgcct tcgcagggca tcaaacagcg
    28801 ccggacgcga gttttcttcc gcccacaccc caaccaatcc gccaggtgaa aactccatgc
    28861 caccgatggt gataccacca ccgccaagct gtttttccgg cgtatcatcg tccgttccgc
    28921 ggtggccgac aaaacccgat tcctctgcgt tgcccggact gccgttgtgg gtatccgtgc
    28981 tgccgataaa gccaagcgga aacggattta cgccaattcg cagctgttcg gaaagcccat
    29041 cgagcagtgc ataccggaca aaatcccgtc gagaaataca tccagtgcgc gccgttccgc
    29101 cgcttcctgt cccttcgcca caatcgggga catttttgcg cagcgtcttt tcaaaatcgc
    29161 actgttcatc gggcgcccca agcactccgg acagactgtt tcgacactct gaatcgcctt
    29221 tgtgctgata aatttccacc acgggttcca tccgctggcg gagtgtagcc agacgccgct
    29281 gctcgtcgag atttgcgccg tctgggtact ctatccggaa catctttccg ttgctttcat
    29341 tggggttgtg cggaatcgca agcacatcac agccggtgcg cgcattgcga cacgcatcat
    29401 cgagcgcttg ccagagcccc tgtggcgtcg gttcttcata gtgcgtaatc ggcagcggaa
    29461 cccgatcatt gcgaaagatg acattgcgat gcagcgtcga caaaccaggg gctccggaat
    29521 actcatacgc gatgaagctg gtgaatccgc acgactcact tttgtcatag gctgtctgtg
    29581 ccgcctgctg aagtcggttc caggtgacag acagcagctc tgcacagccg ctcccatcgg
    29641 gaggacagat gtcttcaaag cgctgatctt cgatcgcaag cttgaaaccc aggttggtaa
    29701 tcgccgagtt tccacccatc cgatagcgct gacaagtcag tgatccatag cctgctgccg
    29761 acggtgtgat gcaggcttgg acttctccca gaaattccga atgatcggtc actgcggcaa
    29821 aatcgagcgg tctggaaaga tgaattttgc gggttccgtt tcccattcca tcgtttggtg
    29881 gcagcgcaat ctcttcaccg cgggcgaaac gatacgcttg ttccggagtc actcgcacat
    29941 cgaatgcgta ggcatcaaaa gacaggctgg tgtgaacgtg aagatcgcca aaataggcct
    30001 gccgcagcgg actgtgtgca gcacaaggag tgcgcggttc ctcatagtgg atgggaggca
    30061 gctcggacgg ttcctgacac gcagccattc cgacgaaaaa cagcggacag tgccacaccg
    30121 cacgcgatag cagttgcatc atgatggaag accttttcca aagagaatca gcgagcgttc
    30181 gatggtccat gccatcgcca gcggacccat cacataccca acccagggga gtcgctgttg
    30241 gcgttgcaaa aaacgcgcgg gcagccacag tgcgagcaca aacacaagct gtccggcttc
    30301 cacgccacag ttaaaagata aaagggaaag caagagctga ccggaaggaa gctccgtctc
    30361 gcgcagtgca gaagcgaaac ccagaccgtg caaaagcccg aacaaaaccg cgagcagaag
    30421 cgggcgcagc atcggttggg atcgattcac acagaccagt tccttcgcga caaacgccac
    30481 actcagtgcg atcagggctt cgacaagggc tgggggcggc gtgactacgc ccagtgcagc
    30541 cagtgccagt gttgcactgt gtccaacagt aaacgccgtc accgtaagaa gcagcgcgga
    30601 ccccgaaggc acaagcagca gcagaccaag cagaagcagc aagtgatcga tcccggacag
    30661 aatgtgatgt acgccaagtc caaaaaaacg ccggagggtc aaagccagcg tcggtgtatt
    30721 ggtcgtatgc agaggcggaa tcaggtgcgg ctcgcgatgc aagagagcgc tgtggacctc
    30781 tctgtttcgg aagtgaatcg tcagcagcgt ctctcccaga tcgcttccct tcatcacgat
    30841 cggatgtcca cgaagaccca tcgcgccaca agacaagatc cgtccggaca gctcggattc
    30901 ttccggaatg gcagttccgt cccgcgacaa aagtgagcag tgcagcggaa acgaaggagt
    30961 catggtctcg ccatttgcgg gcgcttgata cagcacccag aacgtcccgt cggtttcttc
    31021 ggacagcgta accaccgtcg gtgcaaatga gtgcgccagc gccgccgatg gactgatggc
    31081 acatgcaatc cacaaaaaga cgagccagat tcccatcgac ccgctgggcg tgctgtcttt
    31141 tttcatgttc cgcctttttg caccacttca tagcgtggca gaagcgcacc gatggcccgc
    31201 tggcgtcgag cggcgcgatt ctgttctgcg tagtcaaggc ggagccgatc acgcagcgcc
    31261 gaaagggaag gcattgcccc tgcttgtctg ccggtgacct gaatgaaatg cagaccaaag
    31321 ggagagcgca gcggagtcga ccagtttccg tcgggaagcg caaacgccgc ggctgcccat
    31381 gaagcggaaa aaagctgggc aagctcggac tcactgtgca gtggcaagtt tgcgccgagc
    31441 ggatgagggt cgccaagcag cgccgtatcc gtccctgttt gcagcctatc atgcagcgcc
    31501 gcggcgacga tctctgcgcg atccttgtgt ttgtcaaagc caacaaacac ctgtcgcagc
    31561 gcaatgcggg acggcaaaag atagcgcgca ggcgtcgcgt cacgcagcgc cacaagttcc
    31621 tcatcggtag gaatcgaagg cggaatcagc gcatcggcca gatattccat tttctgaatc
    31681 aagcgccgcc gaatgatgat gtcaccgtcg ccaagccgcc ggcgctgcgc ttcacggtac
    31741 agcacttcac tttccacata ttcggacgtc gccgccgcaa gctccgcagc cgtagggggt
    31801 cttcccaacc gccgcaaaag atcccctcgc cgcgccgaga caagctcttt cggaatcacg
    31861 atctgctcac gcgggaccgg acgcacaaac cagtatgcgc agcagagcag accgcccagc
    31921 agcgcaaaat gcagcagagg ttctcgcaac agtgtttgca gaaaccgacg catcggcgca
    31981 tcgttttcca aactgcgcgg cgaggcaagc gcaaaagagg caccgaaaag cagggccgcc
    32041 aagtggctgt ttggttgtgc agtccgcacc gtttgtgtta cgtgcagacg atgcgtcggt
    32101 attggcctct gctgtgcttg ctgtcttttt ttccggggcc actttctgcg catccacagt
    32161 ttgcgctttc gacggtaaat cgctatgcca gactggtcat gatgccgggg cgtgtgcgac
    32221 tggattactc gcttatggtg ggtgaagttc cagccgaacg aatcaaaaga caggctgatc
    32281 tggatcgcaa cggagagctt tcagacagcg aacagaaaca gctcgttgac tcgctcgtgg
    32341 cgacggtcca gcgtgatgtg tcggtgcaga tggatggaca gaaaatcagc gcaccgtttg
    32401 taagtcagcc gctcacgctc aagagtccca aggtcgagtc gactccgttt gcgctggagc
    32461 ttcagacgga tctgccgatt cctaaccaac catcccctca cacactggtt gtggatgaca
    32521 gagccgacat tcccccggtc ggcgagatcg agctgcgcat cgaagaaggg cccggagtcg
    32581 agctgctgtc ggctgaaagt ggcagcacga agccacagtc aaaccaaagt tcatcgccga
    32641 atccgatcca gaacgcaccg actttgctga cggaaacgaa agtccccaca ctgtttcggc
    32701 agtttggacc accgcgctcg cttttgtccg atcgctcggt cacgatgcgc tttcagctgc
    32761 gcaaacagag tccgcaagcc tcgccccgac ggttcacgta tggactgggt gttttttcgc
    32821 tgctgattct gtgtggactt ggactgttcg cccgccgttt tctgcgcagc taacctaagc
    32881 cgactgtgaa aacgaaagcg gcacttcgct gcgcgtcggt tggtttttcc cgtcgggcag
    32941 cagcggcctt gggacttcgg gaatgtctat ttccggatag tagcgccacg ccgccggatt
    33001 cagcatcaag ttgtcccgct gcgcgatctg tggatcaagc cgatgcttgc cttgcaagtg
    33061 ctgcacaatc ttgtgattga cccacaggag tcgcagcatg ctctctgtca tggtcggaac
    33121 actgccgata agcgttgcca gacagcgcag cagccccggt tttttcgcgc tggtctgcgt
    33181 cggatagtca tagcgctgca tggtttcacg attctgccat tccacgatct ctgtccacgc
    33241 cgcagaaagc tcggcgcgga acgccccttc ccgctggttg ggagcacgct tggtcgcgcc
    33301 gacaaaccac ggactcggcg gcagatcgtc tgtctgggcg ttttgcacat gatcgagcac
    33361 cgattcatcc cacggctcgc caacgaagtt caaaatcgac tgcatcgtcg tgcggggatc
    33421 gcggctcaga tcctccaaac ggacttcaag aatcgaatcc agaaacggct tgatgtggga
    33481 atactgaatc cggcaaagca gcccattgat aaacggactt cccgtcccaa acggcatctg
    33541 tccaaacgac tgcaccgtcg gcacaggatg ccgaacgata aagatcaccc gcgcatcagg
    33601 aaagtcggaa aaaatccgcg ccaagttctg ggtgtccagc ggatttttgt cgccaaagcg
    33661 acgcttgccg cgctgcgtgg ccttcgcttt gagaatttcg gcataggcca cccgcgcatc
    33721 atccagcaca agctgcgcag gcagagacgc gaacacatca cgcggatcaa gtcgcatcca
    33781 cgcaaatgaa aacgtgtcga agtaacgcag caaaaacgtc cgcagcggaa tctgtttggg
    33841 cgccagccgc agcaagctat aaaatcccga ttcatgcgcg atgtagatgt tgggatgcgc
    33901 gtttaacatc tgccgaagca gcgtcgtccc agatcggccc gtcccaatca caaagatcgg
    33961 cgaagcagag gggctgtcaa tcgggcgaaa ttcggtctgt aaggaacgca tggcggcgca
    34021 ctatgccccg ctcatttgtc ggctgcaatg ggggcaaaaa ccccacccca ttttcctcgc
    34081 tgacttgtgc cacactcacg cgattcgcag cgtgaatgtg gtgccttgcg gactggttga
    34141 aaacgtgact tcgccaccgt gccgttccgc aaccgcgcgc acaatcgcaa gacccagccc
    34201 ggtgccgccg ctgtcgcgct gcgtggtaaa aaagcgctga aacagccgcg gctggttggc
    34261 cggactgatt ccggttccgt gatcttcgac ttcgatgcag agccgacccg catcagacga
    34321 tacccgcacg gtgacgggcc ttccttcgcc atggcgcagc gcattttcga tcaggtttac
    34381 caccgctgag cgcaggtgat ctccgcttat caagactttg tccggcggac tctggagcac
    34441 aaaacagacc cccggatagc gattcctaag cgctgcaaaa aaatctgcga ccacgatcgt
    34501 ttccgtcggc tctggtgcat tttcaatctg cgcaagctgc aaaagtcgca tcaccagtcg
    34561 ctgcatccgc tctgcatcgg catcaatgtt gtcggcgaac tggcgccgct gcgcttcggt
    34621 cattccctcc cattcatccc gcagcagctc ggcagcaccg cgaatcgccg tgatgggagt
    34681 cttcagctca tggctcacat tggcggcaag ctgtgaaaca taatcggcgc gctcacgcag
    34741 cttgcgcgtc atggtatcaa gcgcggcgct cagacgctgg atctcacttg gcgtaaacag
    34801 gctcatcggc aaagggggcg cggttccacc ctgggcaatg gtctgcgcgg acgtggtgat
    34861 tttgcgcagc ggacggacga tcaatgcggc aaacagaaag ctgacaaaca cggcggcaaa
    34921 cacagtcgca gacacagcaa acagaagtcc gcgtcggttg tgccaaagcg atgtgcgggc
    34981 atccagactg gttcgggaag cacgcaccac agcgatgact tttccatcag aaaagagcgg
    35041 cagcgcgaca aacagacgca ccgatccgcg gcttctgaca tccgaaaacg gcggcagcgg
    35101 ttcatcactt atccgttccc gaatcaccga agcgtagcgc cccacaagtg cgctctcgac
    35161 ttccggaaga ttgtccagac agagccccga ttccccacgg gtcgtcgcca ccacgcagcc
    35221 ttgggaatcg agcacacgca ctgcgctcag gttatagact tgcatccgcg ccagcagcgg
    35281 ttcaagcttt ttgccggcac gcagcacggg ggaatcctgt ggtggcgcaa cccgcagcgg
    35341 ctctggctgc ggcggaagta caccacggcg cagattgctg gtcggttcga tcggaataaa
    35401 cggctccgat tccttccccg gatggcgaaa cggaggcgcc ccaagtgcag gatcgattcc
    35461 ctgctcccgc agccacagtt cgcgccacat ctcaccaacg gcaatcgcct gcgcaatcag
    35521 ctgcctttcg gtctgccgaa ccaagtaatt ttcgtaaaat cgcagcgcga caatcgacaa
    35581 aagaggaaca agcagcacaa acgcattggc gccaagcagc agcgccgaaa tccggagtcc
    35641 gtgtcctttg agcttcacag cgattccttc aagcgatagc caagtccgta caccgtttcg
    35701 atcgggtctg cgccctgtgc agagagcttt tggcggattc tgcgaacgtg actgtcgacg
    35761 gtgcgttcgg tgatcacatg cccgtgtcca tacgctctgt caaccagttc accgcgcgaa
    35821 aacacccgac ccggcgaccc caacatggac tgcagcagcg caaactccgt tacggtcagg
    35881 atgacttcct tttgcatcca cagacagcga tgccgtccca agtccatttc cagcgcgccc
    35941 agcttttgca cgtcccgttt ttccggcgct cccgattctg gaaacagccg ccgcaacacc
    36001 gctttgaccc gcgccagaag ctcacgcggg ctgaacggct tgaccagata gtcgtcggcg
    36061 ccaagctcca gtcccagcac ccgatcgagc tcttcatcgc ggctgctgac aaaaatgatc
    36121 ggggtccgac tcttggcacg gatgcgacga cagacttcca gaccatcgga ttctggcatc
    36181 acgatgtcga gcaccaccag atcgattccc cccgcatcaa atcgcagcag cgcgtccgct
    36241 ccgtccttcg cttcgacaac ttggaaaccg ccttgctgca gcgcaaaccg catcacatcg
    36301 cgaatgtgtc cgtcgtcatc gaccaccaag attcggttca tggaactacc ttactccgag
    36361 cgtagcgttc tgggatcggc tactttttct gcttctctgc cagcgcgata gtaactgccc
    36421 gccagatcca ccgtataggc aattcgtacc gtcgctgcgt tcgacaaaag ctgctggtgc
    36481 agatagatgc tcttgccggg ggtcggaatc ttttgggtgt actccacttc atccccttct
    36541 tccatcgtgg ccagactccc gccttcccag tgatagttaa acacctgcgg cagactcagc
    36601 gtcacataca gatcgtttat gttccacgat gtcgccggaa aggtgtaatc gagccgtcca
    36661 gacagtgcat atccaccctg ctgctcgacg acaagcagct cgactttggc ggattcatcg
    36721 cctgcgcggg ccggctgcac cggaagcgtc acggtcttcc cattggcttt gaacggaacc
    36781 gatgcgccgt tcacaaacac tttttctagc cgctgttttt ccggaatcac aaagtcgatg
    36841 ctgcgcgctc cttcaaactg aagtgcgtac atcagacgga tgatccggcg cccttccagc
    36901 gtcgacacaa ccgatgcgtg ggcgcgattg atgatcggct caatcggggg tcttactgca
    36961 ctctgttttg ccaccaccgc ggctttgctg ctgcgatccc acagcacctc aaagcgattg
    37021 ttggttgggt acagcgtata gccatcgccc tcttcgattc gatcggaatt tcccagcgtg
    37081 aacaggtttt cgtcaaagcg cagcttcagc accgcgagcg ctgcggaagc gtactgcacg
    37141 tcggcactgc gccttgcacc tttgacttgt gcggacagaa gaaagcccac atcaaagctg
    37201 tacgttccgg gcttgtcggt cataaacacc agcatcccat ccacgacggt gagcacgcca
    37261 ttttgaatct gcggcagctt ggtcaagcgc agcgccgcat ctttgcggaa cagcgcagcg
    37321 gacacccagc ctgacgcttc cagaaccgac agctcgacgt gtgcagagac atcgatcgca
    37381 ttttcgagca gccgtccgga cagctcgaac ttgctgatcg tggcgcggat cggtggcttg
    37441 gcgctgggac tttctgcttt gctctcgctt gccttgtaaa gccgcagcac ttccgcaagt
    37501 ggcagcgtgg caaccggatt tcccgacggt ggttgctcag cacggaccgg cgatgacgat
    37561 ccgatggcaa gcattccgca cagcgccaag ctcagaccga aaatgaagtg gttacgcagc
    37621 atgggagtct ccttctgcag cagccggggt gagcagccgc tcgatcagtt cgcgaaccgc
    37681 aggacgagtg gtcagcacca tcatcgtggc gatgagtgcg aggatctcca gcgtgtaaac
    37741 aagcccggtg tagccctgca taaacaccgc agcagttggt acgcacagcg acgatacgat
    37801 aagacccaag acataccgcc gcgcccccgc aggaagcacc cgtcccagat agaacagaag
    37861 cagtccgccc gccaccagca gagaaagacc ccacgcggga tagaagctca tgaacgccgc
    37921 aaagtacgcc aaaaggacaa agaacagcgc gtagcttgcc gcgatcagat agctttcgta
    37981 aatcttcagc ggatgcgcaa agtaaagaca cagcgccgtc agcccgaaga agaacagaag
    38041 gaagggagcc cagccgcgcc gcaccatggt tgcgatcagg tgatcatatg atttttccga
    38101 cggcaagatc acgccgaccg ggattcctga ttgcagcgat tggtagttcc aggacagcca
    38161 cacttcatcg cctttgctgc ggctttctgt ggccggaacg accccatcgg catagtcgaa
    38221 gttgtcccca ccacggatgt gcattcccaa gcggaaattg cgaaccggag ccgctggatc
    38281 gagactgtat gtgaaagcat ccagaccgcg accgcggaac tctacccgaa aagtaacctt
    38341 ggccccggct ttcagtctgc cggtccacag aagtctgtca ctgccacttt ccagttcggc
    38401 ctttgtcggc tgtccatcga cggaaaacag caagttggat agcaggactt tgttcttttc
    38461 catgttgatg ggaaagacaa atgcgatgtc gatgtcgcgc ccctgatcgt tttccgcagc
    38521 gtagtcaccg cggaaactga aatcgaaacc cgaaaagtag accagcccgc gcttgcgata
    38581 gttcatcgcg gcatccactg tgacttcttg ggattgcaaa ggcagcgccg tcaagctgtt
    38641 gaacacggct ccgcttggga cgtatcgcac cgacggacca ggctgcacaa tcggagcccc
    38701 ccagcgatcc atcacatcgc gatacagctc gctgcttgcg aagttgcggc gatcggtcat
    38761 ctgggtcgaa atggcggtac ttaccaccat cacgatgagc agactcgatg cgaagtggta
    38821 tcgggacaac agccggaaca gagagaggat tcgtttcatt gcgttctcct tcgtcacgcc
    38881 gatgcagccg acgtgatgaa agagagccta gcgaacgaaa gtgcagtggc tttggcagga
    38941 caaaggaaag ctgatggaga gattgtgcaa aacttgcgta gagaccaaag tcgcggcgtt
    39001 tccgacggtg aatcggtcta gaagaccggc gccatcaggt cttctttctt ctttttgccg
    39061 cccttttcct tgaccgcttt gccgtctttt ggctccttgg gttccttcgc agcctttcct
    39121 tccttcggct ccttgggttc cttcgcagcc tttccttcct tcggctcctt gggttccttt
    39181 gcagccttgc cttcttttgg ctccttgggt tccttcgcag cctttccttc cttcggttcc
    39241 ttgggttcct tcgcagcctt tccttctttg ggctccttcg gttccttcgg ctccttgact
    39301 tccttcggct ctttcgcggc gacggcttcc gtcttgccct tcggcgtcga tccggtgtcc
    39361 atacttggct cgctcggttt ttcctgcttc gcgagcgtga tggcgatgtt ctggtcttcg
    39421 ctcggatgga tggtgcggct ttcttcctga tacccggacg cgcgaatctt caggctgaat
    39481 tcgccgctgt cctttttcac atcgaaggta tagggcgctt tgacgacatt ttttccatcc
    39541 gggccactct gttcgatctc ggcatccggg ggatctgtct tcaccgaaat atggaccgca
    39601 acggccttct gcggcgcagc caccaccgtc ggagccccac cgcgaaactg cttgacccca
    39661 aacattgaca gcgatccgac cagcatcgct gcggcaaatc cagccagcac cgttgctggt
    39721 ttgcgtcgac ctgccgtctc cccgttggcc cccgacagcg ttgaccgagc ccccgactgc
    39781 gccgcaggac tgggaatcga ttccaccggt gcactcggct gcggcgtcga cggagtctgc
    39841 gcccgcggcc cactgcccgg ctgcaccgcc cccatctgca tcgacggtcc cggcgtcatg
    39901 ctcggcatct gcaaagattg catctgccct ggctgcatca cccccgacgc catcggatgc
    39961 cccggaatca tccccggttg caccacgcct gatgccatcg gattgcccgc tcgcatctgc
    40021 tgctgcattt gctgctgctg catctgctgc tgcatttgca tctgctgcat ctgctgcatc
    40081 tgctgcattt gcatctgctg ctgcatttgc atctgctgct gcatttgcat ctgctgcatc
    40141 tgctgccagt tcggctggcc gtactgaccc ggattgctca tcgcagcctg cggcatcatc
    40201 gccggcatcg ccgcactggc ccccggtgga cgcatcaccc cgctctgcgc cggattcaca
    40261 attcccgacg acatcggccc gcccatcagg ttcatcggtc cgcttggcat catcgccgcc
    40321 gacatcacca ccgatccgct gccaggcagc ggcaccggac cgttggcacg cggcgctggc
    40381 tgaccctgca taaacgactg gaagtgcggc atcgggtcga cgagcgcaag ctgcatgtcg
    40441 ttcatcgact ggaagcggtc ttccctcttt ttgcgaaggc agcgcatcac aatcgcttcc
    40501 agctccgctg ggacattctg attcaccgtc cgaggcgggt ccggctctcg cgtcaggtgc
    40561 gccaccagca cttcgccaaa cccttcgccg gggaacggca ccttgccggt caaaagttca
    40621 tacatcacga cgccgagcgc ataaatgtcg gcgcggtgat cgatgttgcc tttgccatcg
    40681 cactgctccg ggctcatgta cgtcggcgtg ccgatcacca tgcccgtgcg cgtcttttgg
    40741 ctcatcgcgc cgtcttctgt cgtcagcttg gcgataccaa agtcgagcag tttgacgtag
    40801 ttgcgatcac taggccgatt caccaagaag atgttttcgg gcttgaggtc tcggtgtacg
    40861 acgcccttgg catggctggc ggcgagcgcc gagcagcact gcgccagaat gctgacactt
    40921 tcgcgaatcg tgacgccttc ccgtcgcatc cgcgccgaca gcgattcgcc gtcgaggaac
    40981 tccatcatga tgtacttgaa ggtcgttccg tcgacagttg cttcgccaaa gtcgatgatg
    41041 tctacgatgt tcggatggtt gatgtcgttg acagccttgg cttcgttaaa gaagcgggtg
    41101 acgacatcgg ctttgtcgga cagctcgggg tgcagcactt tgacggcgac ttttttgccg
    41161 atctgcggat ggacgcccat gtagacgatg cccataccgc cttcgccgag cttgccttga
    41221 atgtcgtaat tgccgagccg cttgcctatc agagacatcg atccctagcc ctctctggtt
    41281 tcggcagtcc acccgctgag aagttgcgta cccgttcttt ttgcacgctc aagtcgaaga
    41341 tggctgcgta ttcatcatac cacgcgagag cgacatgcgg cgcggtttgc gaggtcatcg
    41401 caggtcgcgc gatgttgtgc agcttgcgta gccttggggc tgtcgggcaa tataggccgc
    41461 tcgcacgggc cccgatccac aatggccctg taatatttcc gactcccgag ggtattcatg
    41521 tcgcaacttg cactcgcgct ggccatcgta tcgaccctac ctgcggccct tcaggcaacg
    41581 cctgccccga cggcagcagt ccctggccaa cccgccgcgt atgttccgcc aggcgcaccg
    41641 accacaccgc ccaccgaaga tgtggcggcc aaagaactct cgaagaagtt cacagcgttt
    41701 ataaagtgtg aagaagggcg caaagatccg ctgatgcact actgcgcact gacgaggctc
    41761 agcagtgatc cgatctggac gcccggccag cccgtgagct acgccggcct gaccgtgttt
    41821 gtgaagacgg gtgccgatct caaaaaatcg gtgtctgagc ctgccgcgct gtcggtgttg
    41881 cacctcggac cctcgtcggc caagctgctg agcttggggg cggcctcgac tcccgatgga
    41941 aaagcgacgg cacaaacgct gcaaaaagcg ctggccggtg aactcaaaga accgccggtg
    42001 ctgccgcagg ccgtgaccag cacttggaaa aacgacgcgc atggtggtcg gacaccgctc
    42061 aagattgacc ggctgtacgc cgacatcacc agcagtccgc cggtgcgcat cttccgtgct
    42121 gaatcgcccc atggtgcggc gtttgtcacc gtcgagacac ttccagatgg acagcgtttt
    42181 tcggtgttcc cactgagcgc cggcattcag taagccgcaa gctccgatct tcaaccaaac
    42241 ccagccatcg agtcaggcgc gtgcggcgaa ttcatcccca aacacagctt cgcgcggggc
    42301 aacccagcac ggcccagttt ttcgcagcga tcggtctgct ccttttgctg ggtgcgggct
    42361 gcaaaaaaga accgccgctc gtcattgatg tgcgggtcga tttggcgtcg cccgctgcgg
    42421 ctgcagatct ggccaaaccc cctgccctgc ccgtggtcag cgtcccgccc caaagcggtg
    42481 aagagcccac catcgagcta agcggcgttg tgcgcatcgg tccgtcgggg cgtccccgct
    42541 atcccgtttc ggtgatcgtc gcgagcgggg actgcacaag ccccagttca cgcctgctgc
    42601 gccgcgttcc gctcagtgac accgacagct ttttcgtcgt tgtgatggcc gatccaggcg
    42661 agcagctcac cgtctgcgcg gcttcagaac cgtcgcccgg tctgcccacg ccgatttggg
    42721 tcaaaagcga tccaatcacc gtcggtccgg cgagtgaaca ggcggtgtcc aacctatcgc
    42781 ttgtgctgcg cagcgccgag ccaaagacgt tttcaaccgc agcgcgcaag aaatgaccat
    42841 gatggcgtac tttcggcatg aaaccgcttg ggtggacgaa ggtgccgaaa tcggtgaagg
    42901 ctgccgaatt tggcattttt gtcacgtcag cgccggggcc aggctaggcc gagactgcgt
    42961 gctggggcaa aatgtcttcg tcgccaaaac cgccgtcttg ggaagctgtg tgcgtgtcca
    43021 gaacaacgtt tcgatctacg atggcgtggt gttagaagac caagtcttcg tcggcccaag
    43081 cgcggtgttt acaaacgtcg tgaatccgcg tgcccacgtc tctcgcaagt cggaatatcg
    43141 ccaaactgtc gtcggacgcg gtgcaaccat tggcgccaac gccaccatcg tttgcggcca
    43201 ccaaatcggt ccttacgcct tcatcggtgc cggtgcggtc gtcacccgcg atgttcccgc
    43261 ccaccagctt tgggtcggcg tcccggcgcg tttcgtcggc tgggtctgcg cctgcggtga
    43321 acggcttccc gacgattcgc tgcactgtgc gggctgcgga agcggctatg ttctgcgagg
    43381 agaaacctta tgcgcgctgt agtccctttg ctatcggctt cgctgtttct gtctgtggtg
    43441 acgtttcaag gcggctgtgc gtcgtcgcag cgcatcgccg atcgcagcca actgcaaagt
    43501 gaactggaac tcgcgtttga cgacggacag ccggcagaac gaccgctctt gccgggcggc
    43561 gacggcgaat ggctgatccg attcgatccg accctgccgg cctacaaacc acagcgcttg
    43621 cgcctgctcg tcgcacaacc gggaaaaatt cgctttgcga tctatcgcag cgatgaaagc
    43681 ggacggcccg gcgccaagct gacgtcgctg gaacgcacct atgaagtgaa ccacacctcg
    43741 agcggcatgg acggcaagtg ggtactcgaa ccgatcggcg atctgcccgt actgagcggc
    43801 acattgtgga ttgggatctc ggtgccgacg accgatccaa ccgcggctcg cttgtgggta
    43861 tcgcgcaaag aatccggcca agtgttccag cgcgacagcg aaccgggcac cgcgctgcaa
    43921 tcaaccaagc tgccgatgac cccgatggtg cgtctgcttt tgtcggcgga acagccagtg
    43981 ccccagcccg cggcaaagcc agcgacggaa acggacccca aaaaagcaac gactggcgca
    44041 gcagcaacga cgcccgccca agcggcgccg caaaaggaca aagatgccaa gtgaatttca
    44101 caaagatcgt ccaagcttcg aagagtcgag cgaggaccgt ggctttcgtg agcgacttga
    44161 gcagctactg cccgacgtgg tgcggcgcgc gatttccgcc ggcagcaccg cgatcacgtc
    44221 gcccgaagag acgatccgtc ggctcgtcag cgaatttaac ctgccgcgcg aagttgcgac
    44281 ctatctgctc gtgcaggcgt cgtcatcgaa agacgagctt ctgcgcgtcg tcggtcagga
    44341 agtacggcgc tttctggaaa cggtgaatgt gtcgggcgag ctgcagaagc tgctgacgtc
    44401 gctgtcgttc gaaatcaaga ccgagattcg ctttatccca aacgatgccg ccgtaaagcc
    44461 cgacgtgaaa aacaaagtca ccgtcaagcg cggcaaagac gacagcagcg atccctaaat
    44521 cagtcgctac aaaagctgga gcggcgtggc ggttaccaga tccaaaaatt cctgccttgt
    44581 cgacggctgc tcgataaaaa tgccacgcac cgcgctcgtc accagccgcg cttcgtgcgc
    44641 ttcgacgcca cgcgctgcca tgcacatgtg cagaccctcg atatagacgc cagcgccgcg
    44701 cgaatcgagc cgattgcaca gaagttccgc cagctcatgg gtcaagtctt cttgcagcac
    44761 cggtcgccgc gcgagcagcc ggatcagccg gcttagcttg ctgatcccca caacccgatc
    44821 ttttggcaga tacgccgccg agacgcgata aatcaccggc agcaggtggt gtggacacag
    44881 tccaaaacag atgttgtgct tgctgatgac catttggtcg tagcgcgccg gaaacgtctt
    44941 ttgcagcaga tcgctgatct cggactcgat ctccgccact ggtcgaacca tctcttgggc
    45001 ggctttggcg gctcgctcag ccgtttcgcg aaaattgtcg tcttcctgcg tcgggtagcc
    45061 cataagtgcg taggccttca gcacttgctc ataggcctgc accaacatct gcttggcatc
    45121 ttgttgtgac atggcgttct ttcgtgtcgt cgcgacggtg cgcgtaggtg tgcgaagaag
    45181 aaaaagtatc gtagcatccg atgccgccat ccgtcagccg acaccatgcg ccgctcgcga
    45241 agcaagaatg ccgcccgctg ccgcgccgtc cgacttgctg tgcaggccgc ggaaacgctc
    45301 gatgcgcaaa ccgcgatgga cttgcgcgaa gatcacgtct ttcggagaat gcgcaccatg
    45361 atttcttctc atcgactcgc cctcgcggtg acggccctct tgacgcttcc gagcgtcgca
    45421 agtgccgcgc cgcctcacga cttttcgatc tacgcgatcc gcatgggtgg ggatgcggcg
    45481 caggccaagc cctacgtcga tcgtttcgcc cggcatcttg aaacggtcac tggctggcca
    45541 gccgcgtcga gcaatggcgc cttcttcacg tcacgcaaag aagcggtggc agcgcttgat
    45601 gcccaaaaac ccggctgggc cgtcgtcgag ccatcgcttt attttgagct gcgcaaatcg
    45661 cacaaagccg ttgccgtcgc ccaagtggtg agcagcgatc tgaacaccgc gcagctccac
    45721 gtcatcgtca aagatccggc aatcaagtcg ctttcggatc tatccggcaa gcgcgtctgg
    45781 acgcagctcg ctgatgccgg caagtacctt agcaacgtgg tgttcgacgg caaaggcgcc
    45841 gccgaaaccc gcttccagct aaagcaagtc ggcgtcgtga ccaaaggcat tcgcgccgtc
    45901 atccgcggcg aggccgatgc aaccctggtc gatcccgatc agctcgtcga agccaagaaa
    45961 atggaaggcg gagccgatct gcgcagcgtt tacacgtcgg aagcgctgcc cggactgttg
    46021 gtcgtggcgt ttccacagct tgcagcggcg gatcaaaaaa agctgagcac tgcgctcatc
    46081 gggatgtgtg cgacggcaaa cggcggtccg gtgtgcaaag agatgcacat ccagaagttt
    46141 gaggccgtaa actcggcgca gctaagcacc accgagaaaa ggtacgaaaa gccatgaaca
    46201 cccgctttgt cacagccttt gggctgattt ttctgtcgct caccgctgcg tgtccgccgg
    46261 cagcggaagt caaaaccgcc gcccccaaag cagcgctgag cgacgccgag ctaccatcgg
    46321 accccgcggc gctcatcaag cttgccgacg aacaactggc gcttggtggc gtcggctttc
    46381 gcaacgcgca gctcgcaatg gaaaaagcgc tcagcaacgc cgaatttgcc ggacaaaagt
    46441 cgtctttcga tgcgcactat cggctcgcgc aggcggcggc tggacagtgc acgctggacg
    46501 aaggtgcggc atgccaagcc ggcctgccga aagcgctgga atcggcccgt cgcgccgtcg
    46561 agatttcacc ggaccgaatc gaaggccatt actatctggc gcagctcatc ggcttttcgg
    46621 cgcagaccca gcgtggtgcc gatgtaaaac cgatgctcac ccagatggtt ggggagtcgg
    46681 agcttgccat caaaattgat gaaaagttcg actcaggcgg accgctgcgg atgctcggca
    46741 cggtctatac ccgcgctcct gccccgccgg tggcgattgg cgatccggaa aaaggcgtgc
    46801 agcttctcac ccgagcggtc gctgcggccc cggagcaccc actcaatgtg atttacctcg
    46861 cggaagccca ggtcgctgac gaacgctacc aagacgccga tgcgtcgatc cagaaagcgc
    46921 gcaagctgct cagcgacgca aagtggagca gccgccgcgc ggagtggaaa gaagtgctcg
    46981 cgaaggtgga gcgcaagctc aaggccaagc aaggacagta agcgcccgcc cagcacggcc
    47041 cacccgatcg cccagacttg acggcgccgg atcgtggtgg caccgtcgtc ttccattttg
    47101 cgaaggaccg tccgatgaga gcgactgttg cacgatttgg cgtcggtgtt ggcctgcttc
    47161 tggcaggctg cgccctggcc ccacccacgc atccgagtgg actgcccaga gttccgcgct
    47221 acacaaactc gatccagctc gactttttgc tcagcagcta tcgagcggtg gcggaaaaag
    47281 cagcgatgtc gtcagaatca gccaagcttc tcgacgaagc atgtctgggc tgggaacggg
    47341 cggtgcttga tctccacggg gcccggctgt atccgagcat ccttcgccaa acgcctgaga
    47401 cgctgatgct cgaacaggtg tggcaggatc ttcccaaaac cgtcgaaaaa tacccgcgcc
    47461 cggcggtgtg tgacaatttg aagattcccg tctcgacgag cggaatgccc aaagaggtca
    47521 cactcggtgt ggtcgcaagc accgtcccac ctgcggcgtc cgcgatgggc tcgggtgatg
    47581 cagccagtcc aacaccgccg cctgtcgcgc caagtccgac ggctccgcct gccgatggag
    47641 ctggcatcat gccgcccccg gccatcagca tgccgtcgac tgccagcagc gaaccgctga
    47701 cgccggtcgc ccgcgagcgt gtcgcgcttc agcgacttcg gatcgcgctg tcgctgccac
    47761 caaacccgcc ggccaatctg ccgagcgcgg caatcgatcc aacgctgccg ctgggactgc
    47821 cgggtacggt ggaccttccg gtgctgcgcg agctggccga aagcgacctt ccgaccgtga
    47881 aactgcgggc gcgctaccac ctgctcgggc tctgtacgct cgcggtggaa accgccgatc
    47941 ggaaccgcac gccgatcagc agcgcaagcc aagccgcgat ccagccggca gatccggtgg
    48001 tttgtggtgc aaacagccca aatgagtcgc tgcgcgccgg tcagcgtcgg ctgctgcgat
    48061 cgctgctttc ggcgtggcga gcccggtaca gcgagccgat ggctgatttc gtcaccagcc
    48121 tcgcaagctt cgtctctcgt gataacccag tcctcgatgg accacgcacg tcccgctgat
    48181 acacattggc cggcagttcg gtggcgaaaa tgcccaattc cgtgctaagg tgcgtatccg
    48241 gtttcccttg ccagcgcgcc ttaccaggcg tgtccctgtc gggcagacgc agcgcaaagc
    48301 ggaaccaagg aatcaaaacc agcagcacaa ccaacacttg accgaactgc cgtttgggca
    48361 aactacttcg cgaaatttcc gattcggcaa cgaaggagcc tcttctccca tgcgcaagcc
    48421 gccgctccgc agtcccaggg actatctcgc tcagcccgag acccaacaca gctacctcgg
    48481 catggaaccc gcccgcgggc gcaccgcagt tgcaggtggt gagtcttttg ccgagctttt
    48541 tgaggcatcg ctccgttccg agaagcgcgc cgtcgccaag cgcgatccag aagtcggtga
    48601 actggtccag ggcgaaatcg tccagatcgg caccgaatac gcattcctcg acattggcgg
    48661 caagtccgag gccatgattt ctctcgatga gctgcgcggt gaagacggcg aactgaccgt
    48721 ccacatcggt gacaagctcg aaggtcacgt catcggctcc gtcggaaaag acggcggact
    48781 tttgatcagc ggtcggctgc aaaaaggcgc cggcctgtcc gatcagatcc gcaccgccta
    48841 tcgccagggc accccagtcc agggccttgt caccggcgtc aacaagggcg gcctcgatgt
    48901 cgacctgggc ggcgtgcggg cgttccttcc gtcgtctcag atcgagcttc gctactgcca
    48961 agatccatcg atctacctgg gcaaagctct ggcgctcaaa gtaagccgca tcagtgaaga
    49021 aagccgccaa gtcatcgtgt cccgtcgagc ggtgctcgaa attgagcagc gcgccctgtc
    49081 cgatgaaagc cgcgcccgtc tgaagatcgg cgaacgctac accggcacca tcgtcgcaat
    49141 gcaagagcca gcagccctgg tcgacatcgg tggtctcgac ggcagcgtcc cgacggcgga
    49201 actgtatcaa gccgcccgca gcgccggtcg cgacgaaaac tcgatcaaga tcggccagcg
    49261 cgtcgatgtc gaagtcgtaa agctcgagga agtgcgcccc ggccacgatc acctccacca
    49321 cagcttccaa ggcaatctgc ggatcggtct ttcgctcaag ggtcttttgg ccgatccgat
    49381 ggcggaagcg ttgtcgacgc tgtccgaagg tgagcgcgtc cgtggtcgcg tcgtccgcct
    49441 ggaaccattt ggtgctttcg tcgaaatccg cacgggtgtc gaagcgctga tccacatctc
    49501 gctgatggcc gagcggcaca tcacccaccc gcgggaaatc cttcagcttg ggcaagagct
    49561 gacggcgacg gtcgtcagcc tcgatcgcga gcggcggcga attggtctgt cgctgatcga
    49621 ggaagcgcga gcggcgcagg cgcaggtcgc gcagacgctg aaggcgggcg atcggatgaa
    49681 agcgcggatc gagcgcgtcg agggcaacgg cctgatcgtg cgggtcatgg tgcccgacgt
    49741 tctgccgacg acattcccgc gcggactgat ccccaacgcc gaagtcggtg ttccccgcgg
    49801 aacggatctg aaaaagctgt tccaaattgg ccgcgaagtg atggcggttg tcatgagcgt
    49861 cgacggcgag ggacgggttc ggctgtcaat tcggcagctg accgaagcgc cgcccgcgcc
    49921 cgccgtcgaa gcagccccgg ttgtgacgga agtggcgacg gccaaagcaa tcgaagccgc
    49981 ggcagtcagc catgaagccc cggcggtgac ggcatcgacg gaaacgccca agagcaaacc
    50041 gaagacgacg cgcgccaaaa aacccaaggt cgccgatgag tcgtcgaccg ccagtgcgga
    50101 aacgccagtc cagcccgccg caccgactgc cgaagcagaa tcgaccaagt cgcctgctcg
    50161 caagaccacc cggaccaaga aaaaagccga agaacccgct ccggcagaag tggtcgtcgc
    50221 agaggtagca ccagcggaag ccccggccca aaaggcaacc aaagccaagt cttcgacgag
    50281 caaaaagacg aaggctgccg cggaggagac gccgaaagct gagcctgcga aagcccctgt
    50341 gctcgaagcc ccagccgaac ccgtcaccgc aaccaagaag cgcgttcgca agtcggccta
    50401 gtctccgctt cgtgttcccc gccggctgtc tcaagccgga aaaagtccat tcagaaacta
    50461 tctttgcgca gaaagtgtgg cgcgttacct tgtaaggggc gctgcatgca ctgcacgtag
    50521 cgccgggctg tactcgtcgg aattcccaca agaggttgtc atgcggtcta ggattcctgt
    50581 tcttgcgctc tccctgcttg gctgcttggg gcttggctgt ccgcccgccg cggatcagcg
    50641 acccgacacc gttcctcctc tcgacaatgg tccagccaaa gagccgatcc ccgaggtctg
    50701 tgacgatcca gtgctgatgg cgccgctgac cgccgaacat gccaactacg gacgcgcgtt
    50761 tactcgctac aaccggctcg agcagcgcta taaggccaag ctccaggcgc gcgagctgca
    50821 agcggcttat gactcgctga tgagcctggg ccggctgtct ctacgacggc tcaacaagcc
    50881 ggatgtcgcc atcgctcact tttccgaagc acgcacgctg gccgcgcaga tcggagagcc
    50941 ggcgaaagag gccgacgcgc tggtctttac cgcgcaggcg caggttcggc ggacgctcta
    51001 cagcgacgca gtgacgacgc tgggccaggc gcaggcgatc tacaaaaaac agaacatgcc
    51061 gctggccgaa gcgcgcacga tggcgctgac ggggattgcc taccaccatc tgaaaaatcc
    51121 gcagaaggcg gtggctctcg tcggtcaagc cgataacacc gtgcgcactg tgaagtctga
    51181 cgatcgccag gtcaaaatgg cggtaggtcg cacgctgatg gcgattgggt cggcgcgcaa
    51241 cctgcttggc gaaacccgac gggccgaaga cgcattccaa gaagcgctcg ccgcgtttgt
    51301 gtcgtcgtct gatttccccg agcaggcgca agcgctcgaa gaaatcgctg agattcacga
    51361 aaaaatcggc aaccacgaaa aagccgcagc gtatttccaa gaagcctatg cggtgtggca
    51421 caagctcgga aacaccgatg gacaagccga cacgctgatc gacctgggcg acgagctgtt
    51481 ccagcttggt cagaaagacc tggcgctcaa ttcgtatatg aaagcgcggg cactttacga
    51541 acagagcggt caagaaggcg attcctcggc ggcgctcgga cggatgggca aagcttatgc
    51601 ggcgctcgga cagaaagcgc aggcgtttgc ttcgttcttc caggcgctat cgctggcccg
    51661 tgcaagtcag cgtccgacgc ggctggcggc ggtgctgcac gatgtatcgg cgacatattc
    51721 gacgatgggt gactatgaac acgcggtgcg ctgcagccag cgggcgttcc agttttggca
    51781 ggagctcggc gagcaagacg acgcggtgcg ggcgcttgtt gaggtcggcg acaactacct
    51841 caagctcggt gatttgcgtg gcgcgcgtga caactacgat cgggcgctcg gaatctgtcg
    51901 cgcgctggtc gatctgtcgt gcgaagccac cgtccgcaag cggctcgatc acgcccaata
    51961 gtctttttgg acttactcca aaggtcggcg catgaaccgc gaatcgcctc cggtagttgt
    52021 tctcggctgt ggttacgtcg gaacgcggct cgcgcagtcg ctgctggccg acggtgtgac
    52081 ggtccgggtg tgcgcccgac ggatcgcact tttggaacca ctgcgcgcgc gcggtgcgga
    52141 cgtccactat ctcgacgggg gaaggccgca tcagtttgcg cccgcgctgg cgggacttct
    52201 gcgtccggtg gtggtgtact cgattccggg cgtgccgggt ctgccgcagg gggaaggcgt
    52261 ccgtcgagcc gccaccgctg cccagcgtgc cggagcaagc gccttcattt acctcagcag
    52321 cagcgccgtg tatggccgca gcgagctgcc atttaccgcc gaatgggtgg acgaagactc
    52381 gccgattgcg attggagatg ccgatgccat ggtgcgtctg gccgacgaag ccgcgctcca
    52441 atcgattgcg cagtcgggtc tgcggacggt gatcttgcgg ctgggtgcga tttacggccc
    52501 agccgtcgat gaatttcacg ccgcgcgcgg tgcccgccag cggctgcgag ccggtcagta
    52561 caagctgtgg gacggtggac ggcactattt ttcccgcatt ttcgtcgacg acttggttcg
    52621 catcatccgc acggcggcag acaaagcacc gcccggatcg acctacgtcg tcggtgacga
    52681 caccccttgt ctgcaaagcg agtacggtac gtggctggcc caccacctcc agttgccgat
    52741 gccgcccgag gtggatgccg ctcgcgcggg cggtcctcgc aacatcattc gtggtcgacg
    52801 gctgcgcaat gccaagctca aacaagagct gggtctgacg ctgctttatc ccggctatcg
    52861 ggaaggcgag ctggcgattg atgccgtcga gcaaggcgcg cccctgccgc cgctgcggct
    52921 ttcggaccag acgccgcttg tgccgaacag cgcgacaaag cccccggcca gcgtccccgt
    52981 tcccagccaa gcggacgcgc cagcgacgat cgccgaggct gtctcgcctg cgccagtccc
    53041 agaagctgtc acggtcgctg ccacaccgtc gccctggccc aagccggtgg acacaagcga
    53101 tctgggactg cttgccgatg ccactgcgcg cggtgtcttc gccatccgcc tgaaagccgg
    53161 ggaagtcgcc gagcgcgggc cggcctacgt gctgctatgc ggccacgcca aggcccagct
    53221 tggtggcgca agccatgtct tcgccgcctg gagcgccgtt ccggcaggca ccgctattac
    53281 cgcgacggaa gactgcgtac ttatccagat cactgcgccc caaaactgat cgcgcggcca
    53341 aaacggatca gctgaccggc ggcccctgca tccagctgtc gagcacgctg cccgacgccg
    53401 tgacctccat tttgatcgtc gagtccggct tggcaacgat gtcgatgatg cacgaatcga
    53461 gcggctgggg tcgtgaaccc ggcatcatgc cttgcgtgcg ggtgcggaaa ataagctggc
    53521 tcgacgaaga tgcggtcgtg tctccgacga gcgccagcgg ctggccagtg ctcgcgcact
    53581 taaggaccgt caccgcgaca gttgccgttg gtgggaaatt ccatccaagc acatccagtt
    53641 caaaaatcgg ctgcatcgtc cgcgggtcca gcgtcagata gcgcttcgcc gaatagatga
    53701 acacatcctt gtcgagcgat ttttgggtca aatcaggccg cggcggcgcc gccaactgcg
    53761 ccagatcgag ctgtacatcg acaaacgatt cctcggtcgt caccgacgaa gaatcgccaa
    53821 tgctcaggat ggtgctgccc tgcgtcgcgg cgatgctgac ggtggtctgt ttgacctgac
    53881 cgctcgactg gatcgacggc tgccccgaca gcaccaagcc ataggtgtac tggacgcgct
    53941 ccgccgaaga aagaccgctt acggagagcg gctgcggcgc ggatgtcttc gattcagcga
    54001 tgctcatgcg atcggagccg cgaatctcgg tatgcgtcat cacgtacagt ttttccgctt
    54061 tgtcgggaat gttgagcaaa atgagccgca gatcaggcgc acatcccgac gaaacgagcg
    54121 cccaaagccc cagacctcgc agcgcatgac cgagcgttgc cgccatgttt ttcccctcgc
    54181 gctagtactg aagaacgatt ttcccaccgt cggtcttgta gagcgttaga cccagtccaa
    54241 caccgaccgc aatcgctgca ccaccgacga tcacaccgct ggtcagaccc caaaaccacg
    54301 gctttttata aagcggcgga tttttgaccc gatcgagctg ccgctgcgct tcctcgcgct
    54361 tgcgaatctg ctcggccagc tccgtctctt tttgcgacag gttctcttcg gcgcgctgct
    54421 tggcctgctg ctcttgcaac agcgtaagct cggcttgctg tttggcttgc tgttccccga
    54481 tcaaaatgtc gagcgtctgc acatagccct ttgcttcggg cacgagcgga ctgttcgggg
    54541 cgacagcgac gaagcgttcg tacatgtctt ttgcatcaat gggacgcagc gctttgcgat
    54601 aggcctgccc agcgttaaac aaaaacagcg gattgggagc ctgcgcgtaa gcgcgtttta
    54661 gctcgtcggc agcgtcggaa aaacgctgtt ctttgaacag tgattgggcg cggcgtgcgt
    54721 gatcatctgc cgatccggtt tccgaagacg gactcgcttt ttccgtcagc gacttttccg
    54781 ctgggccggg ctcttgggct tccgcagccc gcgcaggcgt ggcgacgatc accgctttta
    54841 gcaaaagcgc gaagaccaca agctcacgat cacgcatcag cactctcccc tcacaggcta
    54901 agacacgcgg caagaccaag cgggcaaccc gccgcacccg ttagagacgc cagcaccgaa
    54961 acgattgcaa aactcgttac gccggacgcg acacccgcag cgaaaacacg agaggaatca
    55021 tccccggatg ggcagcaaac caaaatcctg gcggattccc tggccgcgct acaagccccg
    55081 gaaagatatc gaacggcgtg tagtcatact cggcaaagtg gtcgacgcgc aggcccgccg
    55141 aaatcagcgc cgacataagg tccgacagcg gatgattcca gccatgttcg cggtaggtga
    55201 tctttgcttc ccgctcggca tacgttccac tgccaagctc tgtgatcggc cccgcattga
    55261 aataggaata gcggatcgac agatccggcc ccacctgcga catccagaca tagggatgga
    55321 attcgatcag gaaaaacgat ccgccgggct ttagggaatc gtaaaccacc ttggcccacg
    55381 gacgcagatc cggcagccaa ccgatcactc cgaacgacga aaacacccaa tcgaacgttt
    55441 cggaaatgtg ctgccgggtg tcgtagacat tgcaatcgac aaagcgcgcc acgtcggaca
    55501 gaccgacatc ctgggtcagc ttgcgagcga gtgaaatcgc tttgctggaa aaatcgacgc
    55561 cgaccacctt ggcaccccga cgggcccacg acagcgtatc cagaccaaag tgacactgca
    55621 gatgcagaag cgatttgccg gacacatcgc caagcagctc ctcttccaga ggcgtcagag
    55681 atgacttccc ggcgcgaaat ccttccacat cataaaaacg gctctgttcg tgcacatcga
    55741 cgcgcgcatt ccacgccgct tggttatcgg aaaacatgga ttcccaatct tggggacttg
    55801 gcttctcatt cagcatgcgc gcacattact ataaaaattc gcatctggag ccgtcatgaa
    55861 aatcgcgcgc acaattgtcc tctttctgct gttaggaatc gcattgctgg tggtctctgt
    55921 aagactccta accccgtgga tggatcgcta cgggtccacc gatgaggaag tccgcgcaag
    55981 ctacgccggc gacgatcttg tgccgttccc tcgcagcttt gtaaaccatg cagtcaccat
    56041 cttccaaagc cccgatcagg tcttcccatg gctccttcag cttggcgccg acaaaggcgg
    56101 tctgtatagc tatacgaaga ttgaaagact gctcggctgt ccgctcacca acgccgatcg
    56161 catccatcct gaatggcaaa atttggctgt tggtgatctg gtgaaaatgt gtcctcatga
    56221 tcccgcgcca ccaccgtata tcgtcgcaca gatcattccc aatcgagcgc tggtgctagg
    56281 acacaaggaa aacggaaact gggtcgactt gtggcagttt gtccttgctc cgaccaaaga
    56341 aggaaacacc agactgattc tgcgcacgcg cacgatgatg gtaggaggca tgtgggatgt
    56401 cattcaccca ggtgtattcc tcatggaaag aggactcctt ttgggcgtga aggaacgtgc
    56461 ggaaaagtga atttctgtcg atgcaagcgc gttgccctaa acatctgcaa gttgtcaggt
    56521 atccttttca catgcccagc gttttgtttt ccgcagtgcg acgttatgtg atcgctcttt
    56581 tctttctgtc ggcagcgacg gcaaaagccg acccaattcc cgaatcagcc aagcgaatcc
    56641 gtggacaggt gacaagtctg caaggcgccc cgatggccgg tgtggaagtc cgcgcgcaag
    56701 acgggggatc gcaactgctg ggatcgagca aaaccgatgc gcagggaacg ttccagattc
    56761 cgcttccgca gccggctgct gatgtcacat tggtcgtcga agccagcggc taccgacgct
    56821 tcgcacggag tggttttgta cccgacggac agagcaagtc catccagctt ggccgcacga
    56881 tcgatgcgca atacctgagc agcttgctaa aagaaacgga tcccaatcga tttcacgcgc
    56941 aggccgccga cctcctgaat ccggcaccgg gcacgacggg ggaaggtcga ccccaggaaa
    57001 tcctgctgcc gtttctgacc ccgctgtgtg cgcggctgcg gcctctcgta actggaaagg
    57061 aatcacagct caaagagcgc acacttgccg cggaaccgag cgcggtgatg caaatcctgg
    57121 cttactgttc tgaaccagtc gacgactccc tgattgatgc gtggcgtacc ggatccggga
    57181 gctttgcagc gcgtcccaag atcccgtgcc agagcgaaac gctcgacggt gcgatccaaa
    57241 agtggcaggc gctgcatctc caaaacgaag gaatccgtcc cggcgcggag ccgcccatgc
    57301 agatccgcaa acagacatcg ccaagcaatg accgcgcgct ggtttcggtg tcggtcgagt
    57361 atgcgcactg gggctatcgg aatacgctcg tgcttgtacg aaagaaaaat ctgtgggtcg
    57421 ttcgtttggt gctcagcgat gaaatctggc acggcacctg accccacacg cccattcctt
    57481 tccgttcaga aagaacccgc gcaaacgggt cgcactggcg ttgtccgggg cggggagcgc
    57541 ttgcttttgc ggcgcgacaa accgtatcct aaacgtctag cattgtggct tcgttgccgt
    57601 cgctagcgtc tttggccatc ccatcgtcag gcacattggt acggaggtcg gtagatgttc
    57661 gagtccccta gcaagtcggc cagtcgccca cttcccgccg cactgagcgc tccgaccgcc
    57721 gcaaaaaccc gcgcgccaca gccacaagcc ccagtcagcc cggcgatgca gccagccgcg
    57781 aagggaatgc ccgcaccggg tgccatcgaa aaagcgctcg gacattgggc cgaaccaagc
    57841 gccgccgcca aaaccgcact tccgcaaagg cccgctgcag ctgcgctcac gtccggagcc
    57901 gctgcatcgc ctgggtttat gaagtttgct gccggaaatc tcaagcaggg ccggattcca
    57961 ttcccacagg aaggtggcgg tccagactgt caggggccgc ccgaactggg tacgctgtcg
    58021 gcgcgttacg aaagcaacgg agatcctggc accgtatcga cgggaaatgg tgacattggc
    58081 ggggtttcct atggttccta ccaaatgagc acggccagcg gcagcgccgg gcagtttgtg
    58141 tcgggcagcc cctatgccaa cgaattttcc gggcttcagc cgggcacacc ggcatttagc
    58201 gacaagtggc gcgacatcgc gacgcgcgac cctgccggat ttcaagccgc ccagcatgag
    58261 tacatcaagg aaaactacta cgacgtgcag tccggtcgac tgacgcagga catcggactc
    58321 gacccaaaca agcacagcaa cgcgctgcaa caagtcatct ggtcgacatc ggtgcagcat
    58381 ggtgcgtact ccgacatcgt cgaagatgcg ctcgcggggc gcgatgttgg caagatgaac
    58441 gacaccgacc tgataaacgc catctatgca gagcgcggac gcaccgacga aaacggaacc
    58501 ctggttcact tccgtgacag ctcttgggat gtgcagcaag gcgttgcaaa ccgcttccgc
    58561 aacgaaaaca ccgatgcact cggaatgctc gcgaacgagc agacgtgtgc cccaccgcaa
    58621 aatccgtaat ccggaaacgg aagccgaagc gatgcggaat gtttcagcgc cgcggcgtta
    58681 gggcacacag aagcgcggat tgaagacgcg cgccggatcg gaagtgttgt cacactcgag
    58741 cgccgcagga ggccccatgt ccacgggcga gtctgacacc accgcgcgga tcgcttcact
    58801 gccatccgac caagttccca tgtaactcaa atccgcgtcg cagtgtttgc ccgcacgcgc
    58861 acagtcgccc agcgcttggt agttgcgata gacgccgtaa aaatcaagat ggtcgtgcgg
    58921 cagccagctc aaatcccagg tcacgggtgt gaagtggtcg gcgactgtgc ctttgtcgct
    58981 gcggatttga atgtggtctt tttggcttgc agcaatgtca aacgccgaaa atgcgtcgat
    59041 gccaacccga tggtctgctt tggtgtctgc atcgtaggcg atgacctgca cgcgcgcgtt
    59101 tttgggcaag tgaatcggcc ctgtcaaagg cacccaaaac agccatgccg tggcgttcat
    59161 cgaaagccac agcgacgagc ttccccagcc acgcgccgcc gctttgttcg aaagccaagg
    59221 cagcattccc gcaccatagg aatggcccca gattccgatg ttggccaagt ccatgcggtt
    59281 gccgctcacg aaatttagcc agccagcgcc gtagcgaaag ccctcgtcga cctgtggata
    59341 gacaatgctc ggcgcgtaca tcagcgtgta attgacgtac accaccatgt agccgttgct
    59401 caccatgtca tcgatcagat gctggtacgc ttccggaatc agggcgctaa aacccgcggc
    59461 aatgaacacc acagggcgcg tcttgtcatt gcaagttccg ccggtgcggg gtgtgccgct
    59521 gccggtcggt tgaaacagcc acaccttttg ccccaggctg gcaaaaccac tcggtgtcga
    59581 tcctttgcta cagggtccgg gcccaccgag ctgtcccatc gctggttgcg cgggaggagc
    59641 agggtccgca gcatacgcag acggcacaga gacagcgcca agcacagaag ccactacata
    59701 acttgccagc gcgacacagg cagacgttct gcgcatcatg atgatctccc agcgagcgtt
    59761 gtttcagcta ctgcgccgaa gtcccgcgaa tttcgacgat cggaggatga ttcaggttca
    59821 cccacgcggc gatgtcatga atgtcttcgg cccggttgac tgcgatacaa ccacgggtcc
    59881 agtcgcgaaa gttgagcagt ccgcccagcc aaccaaagcc ccgcttggga ccatgaatgc
    59941 cgacgctgcc tccggtgtaa ccagcttgcc gctgcgccgc ggtcggataa ccaatcggaa
    60001 tgaagatcac aaaatcagtc gatgcacgcg gcattcccag cgcataggaa ccaagcggtg
    60061 tccggttgtc tccttccttc tgtttgccga cgccaccgcg tccgattgac accgaatacg
    60121 actgatcgac tttgtcatct ttgcacaacg ccagacggtg aaaatccgtg cggatcacca
    60181 ccgcgttttt cagccctttg cagtcatctg tcgccgtcgc tgcacaggcc gttccacaca
    60241 tcaacatcgc aagtgtcgcc acggtcgaca aacaaatccg cgtcatggtg gcgcaagcct
    60301 aacacgaccc gatttgtcta gccttcgcca accccctttt cacgaatcgc ccccacggag
    60361 attcggcggc gtaaccgaaa ccagcgtgat ggcgctctcg ggacaagcga cgacgcagag
    60421 tccgcaggct cgacagtcct ttgcattggg a
//
LOCUS       DAORGN010000005        59727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_155_length_59727_cov_6.395546, whole genome shotgun sequence.
ACCESSION   DAORGN010000005 DAORGN010000000
VERSION     DAORGN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 59727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59727
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155_length_59727_cov_6.395546"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKL17_01150"
     CDS             <1..426
                     /locus_tag="PKL17_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK43357.1"
                     /translation="PYLDATIKEVLRLRPVIPFVGRVLQQPMQLGGYDLPQGTAVAAC
                     IYLAQRNPDVYPDPDAFLPERFLNGQPDPASWLPFGGGIRRCIGAAFALYEMKIVMGT
                     ILAHTELALENDQPADIKRRAITFWPAGGTRLRKLSHPA"
     gene            480..1490
                     /locus_tag="PKL17_01155"
     CDS             480..1490
                     /locus_tag="PKL17_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43358.1"
                     /translation="MREWGFIAGMIGAAVGVATALGAIGLIVSDQATHDAAVLDTLAQ
                     STVTSDLGDLSDLGDPTSVDLDSPDQSSAPDGNPLGSPVVDGSASDGDSEDSYSEYPV
                     GCFAWSKKRGTALCVVGNFTNECFTHFWSWTLHEIPGENGLTTDIETVTPEDRDKHPD
                     RKESRDHMRAPTTDVQPTETDGEFDHSIRPLASLEPRQTLDVLLAAESGKPSQWLRFR
                     WAQTSAGRNRITLQCLSPGELPPDLASGSARAYPTSEAPPLPKGIRKSEVDVFAYEGG
                     SEGKVVIHQTATSNRLVLEHTQESSAECQHSEAHSAAVVTIGRICTAASVGDAASGLR
                     AR"
     gene            complement(1524..4082)
                     /locus_tag="PKL17_01160"
     CDS             complement(1524..4082)
                     /locus_tag="PKL17_01160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNK43359.1"
                     /translation="MKTSRLSFWRVLPAVVFAAPLGCDPTTVQTPLLADTEVLIDKMG
                     VPHIYAASDRDAMFASGYVMAKLRLFQLEVVRRQARGTQSEVVGEKGLRSDLLARTMS
                     FAQWGQRSRELLRQDYPDEAQLIEAFVRGVNHYIGQVRRGEMPLPPEMGKDGLDFQPT
                     LWTDDDPYIIGKLLSFGMSSSLDSELLASVIKTLAPKFPADFPFCMPTRNAFTMPNAM
                     VQTGMLAPLPLGNPTPPISPELRARAEAALQKAERLSPELGSNNWAVAAKTTASGTPI
                     LAGDPHQPYGSPIRFFVQHINSADKGGSLDVVGFGFAGTPGVQLGHNRSVAWAATTNF
                     ADVMDLWEVPAPSGNRITIGGTDRPAVLRNEKIRVRAEGGKVPSSDDVGDVREYQITD
                     VPGFGVLLPDELLPVPRALITPQQILFAWTGLAATHEAAMYLGLDRSKNLNEWEGAAK
                     RLEVGAVNLVAADQKNIRYRVYAQVPDRGNSHTVGIQPWKLMNGTDPRTLWSGKFLSE
                     DKLPSAKDPDRGYLATANNEPFGFTSDGRVDNDPYYYGYFYDPGDRAARIESELKRLV
                     TRGKITPADLAELQWDARSTLADDLIPALGQSVAELGTDPQLAQFRGRPELIGLFSLL
                     SSWDRQMRRSSSEALIFFAFTHFVAQRALSDDLGPLLPKLFDFEPAYALKPLRLALRG
                     VKGTESLIGTERNLVLMGGLADAADWLKQRFGSVLPPVMKPYQWQDVHSATFDHVLGG
                     KWNAGAFPVDGSVGTVNVSASSMVDAAGKIKGSWSARQGSLFRMIVTFDGSGAPTAQV
                     NFPRGNSGDPGSPYYGNAHDNWLSGKQEHLLFGRSEIESNLAEKIHLRRDGSVE"
     gene            4164..5219
                     /locus_tag="PKL17_01165"
     CDS             4164..5219
                     /locus_tag="PKL17_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43360.1"
                     /translation="MKVVRKLFSPLLSALLLALLTAALLGASSTSQGSSLAAASHPAL
                     SARFSASPVPSTPSPDTMLAPAAPATTFLGMDEDLLLERLRSAEIRSVHFSRGGSSLT
                     LRVEFVDGSRAAFKPQQVHPQSVPRKEAAAYLLSRKLGISAVPPTVMRSLSRDELLQK
                     LDAESQFLRPRLVREAIFDARGLTTGSMMYWVPGLLDLGLDRERAILQWTAELSQNGS
                     LPKEREAILSQLSTLLLFDVIQNNSDRFSGGNILGLRDGSRLFFIDNAFGFQTDPIGH
                     KRALGYLSRCQKFSHTLWTQLQLLRDEQLTFDDPVYGPLLSDEEIRALRSRRDRTLRY
                     LGQLIHSHGVPSVLAFQ"
     gene            5361..5594
                     /locus_tag="PKL17_01170"
     CDS             5361..5594
                     /locus_tag="PKL17_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43361.1"
                     /translation="MSHAKLWFAVCGGALIGLVSTGFSHSTGHESRIVAQCERLPTPE
                     RQNCVQCVTRPVKHHFHPDYPPGARCRPDNGKP"
     gene            complement(5628..7034)
                     /locus_tag="PKL17_01175"
     CDS             complement(5628..7034)
                     /locus_tag="PKL17_01175"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNK43362.1"
                     /translation="MWEIFQSARLRRLFLAETVLLFVVAVLCVRLRIGAGYPGEVPHV
                     EQWAYWREALLAGLTIPVAVQLAMYYLDLYADTPTGSRTQLLVRIHLAHLIGGGALAL
                     LFYVLPVFRLQRLALLMSLGIGQWLLFSLRCHLEETLQSGSWRRRVLVLGVGRLGQQI
                     GRWLQSHPGAGYELAGFLSPQEPEMAQTVVGRPVLASYPDILDAVSRHNITDVVFAPD
                     DPRSEEALLRRLLQVKLLGLQVWNSLSFSAALLHCLPIELLQPADVVFDASFTPSNFT
                     LALKRANDVLLSLFALLLVSPILLVVALLIRIDSSGPALYRQKRVGLRGQLFTIYKFR
                     SMRLQKPGEPMRLTSHNDDRVTRVGRFIRLHRIDELPQFLNVLLGDMSLVGPRPEVES
                     SVSELRQTLPFYDQRHAIRPGITSLGQVRFGAATTLDEARERLKYDLYYQKNMSLAFD
                     LSILIDTVKVVLLKIGAR"
     gene            complement(7151..8509)
                     /locus_tag="PKL17_01180"
     CDS             complement(7151..8509)
                     /locus_tag="PKL17_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNK43363.1"
                     /translation="MNRRDFVKTLTLGSTAAALGTPLYRAFAGPDVASEEFFLFIHAQ
                     GAWDVTVGLDPRNERIGLVEPASTNTIDPAPIRRWTSRTTPLDGTSALSGYSFELVRP
                     ASGPLVFGPAIGELLRHAARLTVINGIAMNTVSHQDGTAYSATGRHLAGSKTVAPSID
                     TMMADSFGATQLLPAVSINYPSSFVGQGLDRRVVPLAISGIGTLAASLRRSTVYESAQ
                     ARDGVTAMLSQEAKDLVGLSANPDVMNGMYLQYEGLRRMNRENLVDLFTDAYLKTKYP
                     NFSYPTMNASVNAAFALESLARNVSRCVSFAYGSFDTHFTNYRQQPLLQQNLFDMLAV
                     MLDYLDVIPHPTKMGSKLSEHTHILVVSDFCRTPMINAAQGRDHYPNNSALVISPKFK
                     GNMSFGKADEEQLLPLPAKAFSDGMRAVTPPDVLATFVGAMGANPRKYLRDGEVIQEI
                     LR"
     gene            complement(8522..9223)
                     /locus_tag="PKL17_01185"
     CDS             complement(8522..9223)
                     /locus_tag="PKL17_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43364.1"
                     /translation="MKTNKRHLKVLLSCLLALSACKDAEPVSPGQTIEQLPGHGQLAQ
                     DTTHKETPRLVPAEAYIRTYLTLFGGLTALQAQAQLQAGGGALFDSWTAYLGALGLPN
                     YATDLSRNTQTNALMVASFERLGVALCDKAVEKDLKGTPPVSVDNRLIFNFDVPKTPL
                     TDADFATRFAKLHRKFLGYPASLAPTDRTARFFALYNDTVKNHNPMGSRFSAVEAGWA
                     AVCQGLIRHPEFQLY"
     gene            complement(9220..11136)
                     /locus_tag="PKL17_01190"
     CDS             complement(9220..11136)
                     /locus_tag="PKL17_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HNK43365.1"
                     /translation="MRDARVSFSRRNGRFRKPLLTLTLGLALWGGHSQAAQPQKTLLD
                     YKYFRSLCIDLLGRMPTEQDLAAFEKADFSLDAWLDAHLQGDTYADRLTRVYSDLLRP
                     QTVSFQVGTSRNTLARILVKDDAGKDVWVYFRPGQRRARHAQVPKASDPNFPKWNNLV
                     RGGFCLLEDETGLKYPDISGAQPPAGTAKPIDKAVLDQYTVVVKPWWLYADYKSTNPS
                     DRYNSATWATRFPGYAITNDLIKESDLKTDTTEVRVCKEEAGIAQSAAMDTQPTMMLD
                     CRTGYGVANSQGCGCGPGLEMCMPAATFIASQPRPTFISSRNTLLGVDDPTDAQQFEY
                     RTWQSLWVAQEPQVFLYNLFLGDRDFREVVSAKYTYVNGPLAQFYKLSSRSNWNDRDL
                     GPTSLPSPDAMPQNFPPHNLLEWRKVDAGPSAAGILTMPLFLFKYGTRRARAHAVYNM
                     FLCRDFVAPAGLQLPPSKEPNLMKREGCAACHQTLEPLSSYFARWVENDWSFLDPKQY
                     PVQNPACKQVSGNIPANCTGRYDNVFSTPTAGMLRGAYGSPDNADAMAQGLGDYLVGR
                     ADFAPCVAQNMAESFLGRSLGAEDSQLRDALTQTLVDGKYRVKPLVKALLTSQQYRAA
                     NNLSSTAWRQGDKQ"
     gene            complement(11200..11691)
                     /locus_tag="PKL17_01195"
     CDS             complement(11200..11691)
                     /locus_tag="PKL17_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNK43366.1"
                     /translation="MASKQSTVDYLLDQMALAGPMTAKRMFGEFGVYLDGKIVALVCD
                     DQLFVKPTEGGRRFIGTPEEGFAYPGAKASFLISGDRFDDSEWLCELIRITARELPDP
                     KPKVAKVPKTSKVSNVPKVPKVLKEAKAAEPPKSAKAKKPADGKASATPRAKLSGRLS
                     AKT"
     gene            complement(11779..12837)
                     /locus_tag="PKL17_01200"
     CDS             complement(11779..12837)
                     /locus_tag="PKL17_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNK43367.1"
                     /translation="MDTVWFASPCESVAPARRLSLVLRIGRYALFPLLVSGCVSTVKP
                     TPLPVPQQAPTSESVSLSSPGRLGNPETTGLGTKPDQKGITGGEKEAGSSLLPNGAPR
                     LPWVNPARCHPVCSFDPGPNLVRINDRAEPDEKGRHRVLRDVREPLEALLVESRAQGH
                     ALKVESAYRSYEDQARVFATIKEIGRAARPGHSEHQLGSVIDLRIPTGAAIAWLSNHA
                     AEFGFVQSYPAQKQRLTGYRPEPWHIRYVGKELAKKVHDEKLTLEELFRAHPDISESG
                     SCADCPQPASRMPCGDVTQAGRCDNTVLSFCYDGSLASVDCASSGQTCGPVLDSLVSD
                     CLPRTDLPKSADSQAIPK"
     gene            complement(12892..14949)
                     /locus_tag="PKL17_01205"
     CDS             complement(12892..14949)
                     /locus_tag="PKL17_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43368.1"
                     /translation="MTRKRRVWVSENFRHLRAELARVRSSVVAAFARLAESTGQNPVP
                     SDSSASEAVNLQLPVETADSQTNNASPPFRLVELCEIYELSDFERDVLLVCAGVELES
                     GIYAQLAKSNHGLAACSLAVLSGLTADADLEALLPEGILRRAQLLRFVPGPALADSTL
                     RIEDRVLHYLLGLHTLDERLRHLLRPLRDVPRTFPSQQPLLKTMETLLSDQAGTEGGQ
                     VLWLCGPDEAARRAVAAELGQSLGLPMFLLDAADLPSRPRERDELVGLWTREQLLEPA
                     GLLLLLDEQDGPDVQRAAAAFLDRIPGVVVVSSRELVSIGQRPVLRIAVPAPTFLEQV
                     DLFQAALTQHAEAESLGADDAARLAYLEQLASCFRLDTQDIDLAVRHAQRLKTSIPGD
                     TQPDALLQDALWQACRERARPQLDELAEVLPPKATWDDLILPDSHLRMLRALTAQQRH
                     RATVYHRWGLGRHSSRGLGVTALFVGQSGTGKTLAAEVVAGALGLDLFRIDLARVVSK
                     YIGETEKNLRRIFDAAEQGGAVLLFDEADALFGKRSEVKDSRDRYANIEVSYLLQRME
                     SYKGVSILTSNMRSSLDAAFLRRLRFVVQFPFPDRSQRVSLWKLALPDSVPQRGVDFD
                     RLAKLSIAGGHIRNIAMAAAFLAAEDGLPVTMRHLLFATRSEFEKLELPLSEADVSGW
                     NEL"
     gene            complement(14980..15909)
                     /locus_tag="PKL17_01210"
     CDS             complement(14980..15909)
                     /locus_tag="PKL17_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43369.1"
                     /translation="MPSFFDKLRRRAPANSQQTQTDVASAAPTQTPASANALMQGGDV
                     AENVDESTLKRQMEQSEIVKLILARFRPGSSREERKDSLDFELHCRGQFVTENYYFVR
                     EFYSFLASTDGRFLEHAKYLYKSYIHKNAGLQINLPFALQTALDNLFAQEIILDRNGM
                     LTLLMAAAEECVNLLRPAKLDFSNKKADRQANPQRTLDDEIDTRLIRDKRISNIKASH
                     QIRWNHQWADRSSRQRDIDRVKQARSFDEYVNKVQTPSRVLGRRNYQLTRKVGRNYDE
                     GTLRDYLRMSGKTVYREFRPLGQVQFEEENDET"
     gene            complement(16036..18087)
                     /locus_tag="PKL17_01215"
     CDS             complement(16036..18087)
                     /locus_tag="PKL17_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43370.1"
                     /translation="MNQNQTANPETKWAFVAEHPESVKTLSDGDAHALGCWLRDTYFA
                     LSVANRASGGDAQFRAIVAAWSPPPTLVLEGLSIYFHPTAGAKLVRFVDEEKIPLALR
                     DKFARPSAQLPHLCAVFVDPEELSYRTYENILPLDRYFSHGSLDATLHDRKRLAKKGD
                     RYSFRLQVSEDTQKRLLSLDELGLYVAPLNSGSRGGDRFIFHAARLSEVLTSALRSAL
                     PKSLLKGFSHVNPVFRANRFAPGDRKFHLHYDSPYYDAAQKQHSRYTLLLYLTSGRGK
                     PTLRIGDDVELEQIDAMTCVLFDQSFAHEGAPYEDGQKFFLRTELVYDCGKVAKDDKI
                     GELFAKACYLTGESVFAPELSRFADDLYNRVARAHFAGLSDEERNAETQYVHKEFKGV
                     HFVASGYDFWFAKGHLTLQECAAIALLDSFNCTISGTAFHKLCRTEVLPRRASETEWI
                     PKLLHTWQKPAEPVFGTLDKGSLFPPPEEAGACCCPFHSISRFDATRCEDIIEFYDRA
                     QSYAKKRVFAAPIVLMGQDIFLDAAQFVVEPGRIQVLSEQSPEPVNFAACWNFGGDPS
                     NYVDVAQTVSVLQPLTPPILYVETSDCYHLMLDFFRNSWRLQSAKYDVPIPFISDIDP
                     GVAEEGGKTPWLSAAKRGNAQRKNGKRGTEAGEGPWWGDTDSAVMAELYPKRKKDRDS
                     D"
     gene            complement(18084..19013)
                     /locus_tag="PKL17_01220"
     CDS             complement(18084..19013)
                     /locus_tag="PKL17_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43371.1"
                     /translation="MDHQAIESRLRGAPSDLSAWDRYSEHLTAQGDLRGRIIRLSQKR
                     GFAPAGSERFAALSAELDRLYAEATPQISGLPRDAKIDWKYGFAVGLQLRLTTAAVSA
                     LHDFLQTRDAVLFRSLRFLPTKNGLPEEEDEEEWSGEGEDVNTPPEPTLLAEAKALAA
                     LDLRSLRVLAMPYCLIGAEGATRLLSSSQMGPLLSLDLRYSYVGDACAQTLAESPQCA
                     GLRSLWLQRCAITAKGVQHLSKSPHLRELRLLDLRYNKIGSKGAVALANSPIVRELSQ
                     LHLYRDEIKKAGVLALAKSSQLPLVLRRMWGQP"
     gene            complement(19088..20560)
                     /locus_tag="PKL17_01225"
     CDS             complement(19088..20560)
                     /locus_tag="PKL17_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNK43372.1"
                     /translation="MHALQTSHKRYPQTESPSLIDLSVGELLTRLRARTLTCRELVEV
                     HIARTKEVNPKLNALVVDRFAQACTEADAADALYDAADHTTLPPLLGIPCSIKEFVAL
                     KGMPHTGGVLHRKGVVATQDATVSARLRRAGAIPLGVTNVPEGGLWMETHNLIWGRTS
                     NPWDVRRTCGGSSGGEGALVGSGATPFGIGSDIGGSIRIPAAFCGTVGHKPSAGLVPN
                     TGHFPAAKDPHGYMVIGPLCRRVADVMPILRVIAGPDDHDPVCQRMPLRDPQSVHIPS
                     LRVFAWHGGSPIRPVMREAIDLSARALLQAGAKEGVLSARDLDGGFMLWASALQAANP
                     EGYESVVSGEGGIPILRELLRYPFGRSNFVLPTLITMLSESILRKLPLGFDALLLRLA
                     RLRESLDAQLGDSGVILHPPYSRPAPRHKLALLTPFDAECTALFNVLGYAVTVVPIGF
                     DEEGIPVAVQVIARQGNDHLTLAAAQVLEGAFGGWRCAMR"
     gene            complement(20580..22340)
                     /locus_tag="PKL17_01230"
     CDS             complement(20580..22340)
                     /locus_tag="PKL17_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNK43373.1"
                     /translation="MRQRTRVCICCVVGLSWVPVLASHADSALSTLPTDKELLGGLLE
                     KPWLSVPDTSRQYFPIFVGATPVQLVNGQKPDSVLRLPAQLRKLADKQADLIRQTLGS
                     RRVELLIWPTQDWNGQPNWVYARYRTSPKEPWRFLVDGENWLPFWNQEVGTKPLLPTR
                     YVPSQPDDASTLFDDFLQSSDLYFQPQFGNCVYVDRSTSQVVLKWPHPDSQSRHEATL
                     RTPGRYLAYCMNQPARHGSEPLAMVLAVENGKLDWPAIDWQNPPVSPGKIDEPLRIAS
                     YPVDFEAQFRKDLRIFSGVDPVVLPKSGVSRRFVKKGSFQPDHDLEHMVDYLEQRYAR
                     LGIQTERQRFLFRNIAQSNLIAKLPGIPRADGKPLPKVILADHIDSAVEEDTFEKTGQ
                     RVTTPGADDNVTATAVLLRAAVALRTLPRNADVWLVHLTGEEFPSDDLGAWRFAEKLL
                     ADRVEVRALLISDFIGWHPAGDRSFQINATSHPLSAQHAALALDASAKLAPSLRSHYV
                     PRHTVRSAVYQTDVQVFEFLGFPGLLFNEHIDYRRADQQSPHNHQSSDTVENLDIPFA
                     AEIAKVMIETVARLANLPAP"
     gene            complement(22344..22883)
                     /locus_tag="PKL17_01235"
     CDS             complement(22344..22883)
                     /locus_tag="PKL17_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNK43374.1"
                     /translation="MPIIRSFRGISPLVHESVFVADNATLVGDVHIGERSTIWYGAVL
                     RGDVHHIRVGSETSIQDNTVVHVTHGRFPAEIGDRVTVGHSATIHGCTIGSHCIIGMG
                     STILDQVEVGDHCIIGAGSLLTPGTKIPPGHLVVGSPGRIKRPLSQDELSWIEFSADH
                     YIELCAIYRAEAESQKSGG"
     gene            complement(22951..24300)
                     /locus_tag="PKL17_01240"
     CDS             complement(22951..24300)
                     /locus_tag="PKL17_01240"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNK43375.1"
                     /translation="MAGAGAQVKRILFRGAQAFVDGKQSQIDLLVEDRLIAAMAPADS
                     LSASPSDVVVDASGLFLAPGFHDIHVHLREPGQSHKETIGSGTRAAARGGFARIACMP
                     NTRPVLDSAEQLQSLRKRLWTDAVVAVDVIAAVTMGSAGDVLTDFAALRSAGAVALSD
                     DGRGIQRAAVMRQALLRAAEVGLPIFAHCEDESLSHLGVIHEGATARDRCLVGIPSEA
                     EAVHVARDIVLSEKSGAHYHVCHLSCAQSLELVRSAKARGLRVSCEVTPHHLLLSDSD
                     IASDDGNFKMNPPLRTESDRAALLAGLCDGTVDAIATDHAPHTPAEKARGLAAAPFGV
                     IGLETAFPLLYTELVLRNLLSLERLVELLTCGPARILSSPIPKLAVSAPADLVLLDLK
                     TERVVRVEELRSRSRNTPFLHRPLRGWPVLTLCQGQVAFSSLAQLPEPVAAPSTSAL"
     gene            complement(24276..25292)
                     /locus_tag="PKL17_01245"
     CDS             complement(24276..25292)
                     /locus_tag="PKL17_01245"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNK43376.1"
                     /translation="MQAELQKDTTPFRVPDRTLATPVDPEGTQPARNLIDVDALSNRD
                     IDRIFRRAAALREASTPLGNMTVQTLRGHIAAMLFFEPSTRTRLSFELAAQRLGAAAI
                     PLEVSRSSIEKGEALYDTLQTLVALGVTIAILRHPDSNVYERLCGRSDISLLNAGHGV
                     MAHPTQALLDGFTLERRLGCLQDKTIVLCGDIVHSRVARSNLRMLQRMGARLILCGPP
                     QFLPSLDDIPQLTEVRIAALDDVLESADAIMCLRIQRERHVPDAGKSDQPIDYLQEYG
                     LTEERFARTHPDCVLLHPGPVNRGVELAAELVEDPRSLILEQVKNGLYIRMAVLEWLA
                     RVLK"
     gene            complement(25305..26477)
                     /gene="purK"
                     /locus_tag="PKL17_01250"
     CDS             complement(25305..26477)
                     /gene="purK"
                     /locus_tag="PKL17_01250"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0034028 - 5-(carboxyamino)imidazole
                     ribonucleotide synthase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNK43377.1"
                     /translation="MKRSGAILPGAVIGILGGGQLGRMTAMAARSYGYRVQVLDPEPA
                     CPARFVVDTCICADFGDARAAAGLARGSDVVTVEIESIPSDALRAADSYAPLRPGISA
                     LSLVSDRALQKKWLSRSGFPCAPFQVVSDVAALADALQTFFPDCFVKRSQGGYDGRGQ
                     LRCGTAADAAQVFDKLGPGTLVVEQAIPLAAELSVLVARRPRGDIAVFPVAYNHHVGG
                     ILRWSVLPAPQEEIPFRLSEQAQELARSIAEQLAVEGLLTVELFVNREGALLVNELAV
                     RPHNTFHTTERATCVSQFEAHVRAVCDLPLGDVSLVKPAAIVNLLGDLWLADRPLDVA
                     QMLDVPTLRLHLYEKAKPKPGRKMGHLSALGETPTKARDRVLHAYQILTQTIDPKL"
     gene            complement(26477..28258)
                     /gene="purD"
                     /locus_tag="PKL17_01255"
     CDS             complement(26477..28258)
                     /gene="purD"
                     /locus_tag="PKL17_01255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNK43378.1"
                     /translation="MNTPKRRVLVIGSGGREHAMIVALAQCEDVTLFCAPGNPGTASL
                     ATNLDASATDVAALAAMAKAHAIDLVIPGPEATLSLGIAGELARVAIPCVGPSQAAAR
                     LESSKVFMRELTAPLRIPAPQCVVVRSVSELSAALGRFASPPVVKADGLAAGKGVFLP
                     DDFDECRQVAAALLEGKLGEAGRQILIEERLCGVEVSLFFACHHETAVALPHARDHKR
                     LGDSDEGPNTGGMGAVSPNPAIDADGVDKVQREFVLPVLRALRQKGCPFVGFLFVGLM
                     MTADGPKLLECNVRLGDPEAQAILPRLGKGEFLRLCEAMVRGQLEHFVLNQLPDATCA
                     VVLAASGYPEVARAGDEITIVADALQKSGASLVYGGTQKQNGVLTTAGGRVLTVVGQG
                     KTPCLARRAAYAGVSAVRFSGMQYRRDIGGRETENPWVSVIMGSTSDHSVMSEATALL
                     RELEIPHEVRVVSAHRTPEWMFQFAKEAESRGLEVIIAAAGGAAHLPGMVASLTTVPV
                     LGVPIAATALQGMDALLSIVQMPRGVPVGTLAIGAPGAANAALLAAEICALSRPSLRD
                     RLHRVRNSQRDRVLAVTDSLNPSQGSA"
     gene            complement(28270..29844)
                     /gene="purH"
                     /locus_tag="PKL17_01260"
     CDS             complement(28270..29844)
                     /gene="purH"
                     /locus_tag="PKL17_01260"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNK43379.1"
                     /translation="MDQRSAARYALLSVFDKRGLVPLASALVSQGMTLLASGGTAQHL
                     LSAGLSVIPVEEFTGAEEILEGRVKTLHPKIHAGLLVDRRKPEHLAALEKSGYVPIDL
                     LVCNLYPFAETVARGQSPAQIIEMIDIGGPAMIRAAAKNHAGGITVVVDPADYPQIIA
                     AIEQGPLPAELRQALAARAFAQIAKYDQQIADWFARPSLDDAAQESRFPLQLGPFVRT
                     QALRYGENPHQEAFLYRDPAEPIGAAHATPLQGKALSYTNLLDVDAAYRAVFGLRTLF
                     GRPACTIVKHAAACGMAVADSQVDSYLRALSGDRQSAFGSVIAFESELSETTAQAMLD
                     SKLFIECICAPGFSVGARALLAEKTNLRLLVLPASDPQPRVSFHRIGGGLLLQAVDSG
                     VSDPASWQVVSRRPLDPAWTAELQFAEYAAMTLRSNAISVSKGLTLLGAGTGHVSRVD
                     AATLALHKSAKEARGAFLGSDAFFPFPDCAEAAAAAGIAAIIQPGGSVRDAETIAVCD
                     RHNITLVFSGRRHFRH"
     gene            complement(29849..30511)
                     /gene="purN"
                     /locus_tag="PKL17_01265"
     CDS             complement(29849..30511)
                     /gene="purN"
                     /locus_tag="PKL17_01265"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNK43380.1"
                     /translation="MPAANRPRLAILGSGRGSNAVALLDAFRLGMLAADLVLVVSNVR
                     AAPILDRAKERGVPTVCIPHAGVSRSSHESALSAVLSAHRVDHLLLAGYMRVLGPDFV
                     RGFAGHILNIHPSLLPDFPGAHAVADQWAAQVKVSGATVHFVDEGIDTGPILLSGSLL
                     VRGDEGVDGLADRIRLEIEHELYPRAVQLFLDRLQNGRPLHQGIVRKPGGLAAPTRFE
                     TE"
     gene            complement(30498..31532)
                     /gene="purM"
                     /locus_tag="PKL17_01270"
     CDS             complement(30498..31532)
                     /gene="purM"
                     /locus_tag="PKL17_01270"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNK43381.1"
                     /translation="MKPPALTYRDAGVDLDAADRFVAFVRKHAKLTYGNAVVDSGDAY
                     AGLVRPPLSGLADPLLAATCDGVGTKLLVARDCQDYSGIGQDLVAMNVNDLLPRGARP
                     LFFLDYIAAGRLDSVPLADIASGIIAACQQSGCALLGGETAELPDAYREGDCDLAGFA
                     VGIVDAPHVPKADISDGDLLYAFPSSGIHANGLSLARRIVADAGLSYQDTPSELPHSL
                     AKELLIPTRLYVQDVLSLHKQVRVKAAAHVTGGGLLLRAHKLCPPGLRLSIDPDSYVR
                     PAIFSLLARLGGVSWEEMAKTFNMGIGFLLVMGPSDAETLSQVSCGDLRPVGKVVRGE
                     RGVDLGYARS"
     gene            complement(31529..32971)
                     /gene="purF"
                     /locus_tag="PKL17_01275"
     CDS             complement(31529..32971)
                     /gene="purF"
                     /locus_tag="PKL17_01275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNK43382.1"
                     /translation="MLMRSGLPVLSSQTDKPREYCGVMAVYDPQGLAARFVHRGLVAL
                     QHRGQEAAGLAIVTELGSLHAIRGAGLVRQVLKPSVVASLSATRAIGHVRYSTVAEEH
                     PDNHQPICADTPFGPLSLAHNGHLRNAKELTAALVAAGVTLCSTMDSELFVALVARSR
                     ASSFAAALKDATESVVGSYSLAMCCDGRIYGLRDPHGLRPLVIGVLAGSPSGFVIASE
                     TCALDALGAQFVCEVGRGELVEVGPQGIVKTQLLAPSPAPAPCMFELIYFARPDSVVF
                     GIGAQRARIEMGRELALQDRDLPMPDLVVPIPDSGVAAATGYAQTSGVPFEMALLRSH
                     FAGRSFILPNQDARRQALLSKLSVVAESVAGKRIVLVDDSLVRGNTARFVSELLRERG
                     AKEVWLRLASPPIVSPCHLGIDMASPDELLFHVCQESAQRTTPPQDPIDVLRQQIGVD
                     GVRFLSQAGLRRATQQQPFCLGCMTGEYPT"
     gene            complement(32965..35214)
                     /gene="purL"
                     /locus_tag="PKL17_01280"
     CDS             complement(32965..35214)
                     /gene="purL"
                     /locus_tag="PKL17_01280"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNK43383.1"
                     /translation="MKKSRSASDVQDHAAFPPEGMLDEETARRLAERMGLLESEYAVI
                     RKRLDRTPTHSELAVFSGMWSEHCSYKSTKHLLSTLPKDSPHVLAGPGAHAGVVSVGE
                     GWAVAFKIESHNHPSAVEPYQGAATGVGGILRDVIAQGARPCALLDFLCFGSPDTADT
                     KRIASGVVSGIGGYGNAIGVPIVGGRTHFDPAYEGNPLVNAMAVGLVRPHELRHARAQ
                     GVGNVLLLVGAATGRDGVLGAAFASEALGESSSFAQRRSHIQIGDPFSGKRLLEAILA
                     FGPAQGLLAVQDLGACGIACASFEMAAPCGVGIDIQLDDVPLREPDLSPLEILLSETQ
                     ERFALVIEEKNLATAIAHFQGYGLEAAAIGRITGSGLVRVFHKGHCHVSLPAQLVAGG
                     APKSDWPLCETPLATAPIDDVKAGDDKSHIECLLYLLSALSDPDPIYSRFDQTVGNRT
                     VRGPGQAAAAVMRLPNSRRGFALTLTGDGNLCAADPYAGAQAALAESVRRLGCVGAEL
                     LAITDGLNFASPRDAKKHRQIVEVIRGLGDGLRTLGVPVTGGNVSLYNESPRGPIPPT
                     PMIGGLGVIPDVGRVPVSEFRSGQAVFVLGHLSSRQCASRLARLLLGRPCGIPSVDLA
                     LEQRLARLVCSAISDRLICAASAVTAGGLATALAKMCLRSQCGIVAELPETDAPRWLL
                     FGEHPAQALVSTWDDDATARLLLLARQHEIPIHRLGTAQGSVLRIGADITLRLTEIES
                     ARKSRAVAC"
     gene            complement(35211..35900)
                     /gene="purQ"
                     /locus_tag="PKL17_01285"
     CDS             complement(35211..35900)
                     /gene="purQ"
                     /locus_tag="PKL17_01285"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNK43384.1"
                     /translation="MSVRNPIKAAVIVFPGSNGDRDLYEALCAAGFDTQYVRDTDDIP
                     GDVALCGLPGGFSYGDYWRAGVLASRARAVRQLASFHASGGLVIGICNGFQILAEAGY
                     LRGALTYNTPPRFVHRFVTVVVRETAERSPWFSTLPVGTKLTLPIAHAEGAYRLPKDG
                     HSVLPRIPLCYEHNPNGSQLDAAAVSDETGRILGIMPHPERAIDLLLGSQDGLRLFES
                     AFRFLQRVQTP"
     gene            complement(35897..36196)
                     /locus_tag="PKL17_01290"
     CDS             complement(35897..36196)
                     /locus_tag="PKL17_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNK43385.1"
                     /translation="MFRFELVVTPVVGARDPQAEAVTSALAHSDFSSVRADVVGRYLR
                     FSVQETDADRALLLADKVCQQFLVNPNLETYSLRLLADPPAVAAERATDSGAGAS"
     gene            complement(36199..36954)
                     /locus_tag="PKL17_01295"
     CDS             complement(36199..36954)
                     /locus_tag="PKL17_01295"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNK43386.1"
                     /translation="MLVHAAVYEGKAKRVRISDDGLAEIEFKDDVTAGNGKKHAVFLG
                     KGILCCRMSELLLAYLQQSGTPTHFLRRTADNQLTVRRVEIIPLEVVVRFEVAGSLQK
                     RTGLPYRARCEPPVYELYYKRDDLGDPLLNEHHVRLLNLATTDELRTIRELALSAAAK
                     LHSLLLQAQLALLDLKFEMGRSDSALLLADEISPDTCRLRDLASGEVLDKDVFRNDLA
                     ELLPAYREVLCRLEKLPALRSLGLSPNSGGKES"
     gene            37189..38103
                     /locus_tag="PKL17_01300"
     CDS             37189..38103
                     /locus_tag="PKL17_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNK43387.1"
                     /translation="MRNVHSRMISALSGPLVVAAGPWSSGECTGEPLSAADAVVAKTV
                     TSAPRSGNPPPVLRTLPPANGQTPAGYLNRIGLRNVGAQAFVRDRLPLVQRCGRPIIQ
                     SFTAQTEAEVEEILAQLEPVSFLGYELNASCPNATSGLLSTQALRTVLVRARALTQRP
                     ITVKLAYQSADELLAKARICEDCGADALTAINTMPALDLSPIPLASESQFVGGLSGPG
                     LNPFAQWAVAQLVREQSLPVIACGGIHSLRQVLAFCALGAQAVQIGSVLIEDPQRVTT
                     LRAELFAAYKQHQVDSFAALGERLCQPH"
     gene            38088..38885
                     /gene="pyrF"
                     /locus_tag="PKL17_01305"
     CDS             38088..38885
                     /gene="pyrF"
                     /locus_tag="PKL17_01305"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNK43388.1"
                     /translation="MSATLKLTERLHCRGPLCIGLDPDPRLIPSCVHGDVGVFLREII
                     LATAEFACAYKVNAAFFEALGISGMHLLERVRKLLPSDSYLVWDAKRGDITNTNRHYA
                     KSAFSVWNADAVTVSPYLGFDSLSPFFDYSNRLTFVLCATSEGTELQELETLPDRKPL
                     WLWVAKQAAKRSRGQIGLVVGATHPDRIRAVREVAKDIPLLVPGVGAQGGDIPSQPAL
                     VNVSRSILYASADADFAACAHAAAKSFHQRLISAAKEPPHHDHSAHF"
     gene            38905..39465
                     /gene="pyrE"
                     /locus_tag="PKL17_01310"
     CDS             38905..39465
                     /gene="pyrE"
                     /locus_tag="PKL17_01310"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNK43389.1"
                     /translation="MLLGKTEAVLHGHFRLSSGLHSDTYIQCARLLSHPAYAQQMGHD
                     IARKVQKSGVGPIDAVIGPALGGIIIAHEVARALSVCSLFAERQGSDLALRRGFSIKK
                     GQRILVVEDVITTGKSAKETASLVLALGGVVVGYACIVARGDSHALSPLHCLCQLRPQ
                     VFSETECVLCQEGVPLHKPGSRPEAV"
     gene            complement(39474..40187)
                     /locus_tag="PKL17_01315"
     CDS             complement(39474..40187)
                     /locus_tag="PKL17_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43390.1"
                     /translation="MLSLVVPRLVYADSILDVVAKSTVPWEKYKEKHGVAVERRAVKG
                     SSFYEYRAVSTMPMAPAALIELMWSTVPTRTGPVVRKRQVLQKSKDSLLIYDQIKTPV
                     VSDRDYTMRLRTQALGNDRYEMTFQTANDEGPPLDPHYVRIPAISGRWLVEPEPGKDG
                     FSHVTYQTYSEPGGSVPAFMISGPQFEQVIRSIENLAAHIKSEQAGSQGTSGQQAQPP
                     HTEHGELPEPMKNPPPPPT"
     gene            complement(40298..40669)
                     /locus_tag="PKL17_01320"
     CDS             complement(40298..40669)
                     /locus_tag="PKL17_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK43391.1"
                     /translation="METPSGFAKTARVELDCQRCGACCVNAKENEAEGRTDYVSVEPS
                     AKLLLRRDLVRKYVVMDDMGHPHLRLASDGRCLALRGVLFKKVSCELYHHRPKACRTV
                     QPGDGDCLRARKERGMTDDDR"
     gene            complement(40685..41608)
                     /locus_tag="PKL17_01325"
     CDS             complement(40685..41608)
                     /locus_tag="PKL17_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNK43392.1"
                     /translation="MDLEELRAFLAVADSGSFLTAAKSLRLSRATLRRRIDQLEARAG
                     VLLVDRTRVGVMLTEAGRVLAARGRLMVQEASALIASVREVGSEPSGMLRVLLPIGLP
                     PHLLTPMLGFVHHKYPRLSFHLQFSDDPTGGLIEDVDLAFHFGDASPSGAWVSKEIVR
                     SRVWAVASRDYLQQRGTPRTLDDLKHHDLMTWVGPGDDRKVWPLLRGGSYPVSAMTVT
                     RHIHLIRQFAIAGMGIALVPDAMLPDPGVPDGTLVPVLPDLIGKDVGMRVMIPAVLWE
                     IPRIRAVIDVLRPFLGELGVENILTPDPAKK"
     gene            41698..43047
                     /locus_tag="PKL17_01330"
     CDS             41698..43047
                     /locus_tag="PKL17_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNK43393.1"
                     /translation="MADETRNPDGFGKLLWRGESGYETARRRSMWNQRIPDRFPDAIY
                     RVHDVYDVVSAVKLAKQRGLNVSVKSGGHSWSGNHLREGGLLIDMSAMQAATIDKAAM
                     RATTEPGKGGSILAEMLLRQGLFFPTGHCRGVCVGGFLLQGGFGWHSRTLGPACMSVI
                     GLDLVTMDGDVVHASPTENADLYWAARGSGPGFFAVVTRFHLRVYPRPRVMGVAYQHH
                     PMEQLEDVYRFAHRVAPDVPNSIELQFIMSRRASFLPKPGIEILAPIFADSVKEAWRA
                     TEFLRDAEIGRHATLRVPFLPATLSMMYTAAMQHYPDDHRWAVDNMWTRAPIDDLLPG
                     IRRIAETMPEEPAHFLWMNWSPPSNRPDMAFSVEDKTYLALYGGWKHAADDEKNIPWA
                     TDRMREMAHLSTGCQLADENLRIRPSRFLTEANMQRLDKIRHDRDCDLRFHSYQGRP"
     gene            43044..43892
                     /locus_tag="PKL17_01335"
     CDS             43044..43892
                     /locus_tag="PKL17_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43394.1"
                     /translation="MNHPHPKRILQLGISQAEQRQKPYGHFYNPKLGALPSHAKEALL
                     IGPVAGPVLPTLADAPLLLTCEETELENGLGISADGCLHVAIRTDLPDVTPAMIDWWF
                     GWHSAEPQRYKLWHPRAHVYAAWQDVETQELAAQKGRPRYVGRVSFVDEYLGSTLTHA
                     AIGFVPPKTLGFDEALLADAQKHTVIAARVSLAETPLDAGYLVHHVRKVPGGSEMRSR
                     FWLGGPYAAVQAGNRRVQIPSAVSRRIRAPTLDEGRDLLVHCSQEMSHLATFLPKLYA
                     EFFDVA"
     gene            43991..46981
                     /locus_tag="PKL17_01340"
     CDS             43991..46981
                     /locus_tag="PKL17_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNK43395.1"
                     /translation="MEEFVLEFARAEQAGDPFAFRFTPQPYLLRTKGGGFQSAEFPWS
                     EALLDDLESLRKPRPDRAIVQRIGELLRRFVTPLGVEAQLDQIVTASRAGKEVCITFR
                     SAAAELYALPWELLADRHSGQAIGSLPGVLFRYEWPESKTLQSTVRSEGGRILFAWSA
                     AQGAVPAKEQIAAIRSACEKESLPFDSESDVIAHASCDKIVSALEKARATSRPVAILH
                     ILCHGGEQGTTFGLSLNGEEEGEPEHVDGATLRQLLSPFADMVQLVVLSACDSGNGGA
                     LGNQLGSVAQALHRAGFAAVLASRFPLAIRAANQLAETLYEGLVCNSLSVEKALLSVR
                     NRLVRDPALFDWASIQLYARTGDQGDLRPLLFRPYRGLLAFQPEHRAFFFGREREIDQ
                     VLTALSSLRSAGKPKFVIVAGASGSGKSSLVLAGVIPKLCGQGPDRFLSASVKPGASP
                     LEVLKQIPERTDRPLLLVIDQLEELFTHVPDPAVRTQFAQKLWLLAQRPDVHIVATLR
                     VDFIGECGELVIDESGLRLDRIAYDDAHRVFVAQLGPKELLATIEQPAARVGIALEAG
                     LTSRMLEAVSGEPGALPLLQHTLDQLWLRREHRTLTQKGYDALGQLGGALSQHAESLF
                     GKLSQEEQRSAQEVMVRLVRLEDGMTRATRQRVLLDKLRPRKKESQARFDGVLRDMTA
                     ARLLTIAGEDPNSTVEVAHEALIRSWPRLQNWLKEDQTFLIELGKLEGLLREWKEHHA
                     LLVSDQLRFAERLLRTYPERLPEETAQLVRESQREERKIRLIRLTALLVLVCGLIVQS
                     WMFFSEQRAHKRAELARHELINLMSKTSQLPSRAELLRTLARLRLGLELRPDLALVAI
                     AEAQKLEPENPRLLVDQAEYFLAAARFDEVFLPAQIAFEKEADPARKVLSAAIAWSAA
                     RLHHRPIEQKAWSERLQNTFHSLPEGVEVPELYATSQLLIENQSLLRQALPIPDVLAV
                     FKLIHSPRTPQREDDLAKRLRVGL"
     gene            47081..47854
                     /gene="surE"
                     /locus_tag="PKL17_01345"
     CDS             47081..47854
                     /gene="surE"
                     /locus_tag="PKL17_01345"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNK43396.1"
                     /translation="MKLLLTNDDGVRADGINVLASTLSDVAEVFVVAPDRERSATSHS
                     FTLFDPLRVEKVGHNVWSVSGTPADCAYLGVLEFCKGADLVISGINHGYNLGADIFYS
                     GTVAAAVESALRGYSAIAMSMGMGRGADFHRAARFCRALIQGMQGRRLPPRTLLSVNV
                     PGPKEPPPPQDGPAYQITRLGERMYRDHVDSRTDPRGNTYYWIGGPVATDSEPPGTDV
                     HAIHQGLISVTPLGLDLTHRQLLGDLPSWDVPGYRQIGT"
     gene            47881..48435
                     /locus_tag="PKL17_01350"
     CDS             47881..48435
                     /locus_tag="PKL17_01350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNK43397.1"
                     /translation="MKPFTTLSQELLADYRIFRLRKDRCRSPRTQGEHDFYVLEADSW
                     VNVVALTPDEQIILIEQYRFGVGQITLEMPGGIIDPGETPLEAAARELIEETGYQADA
                     ISVLGHVDPNPAILNNRCYTALALGCRNIAGQSLDEKEDIAVRLLPIADVPQLLASGQ
                     ITHALVWAAYLHYDLWKKKQGSAR"
     gene            complement(48442..49590)
                     /locus_tag="PKL17_01355"
     CDS             complement(48442..49590)
                     /locus_tag="PKL17_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNK43398.1"
                     /translation="MVSLLLPLFASCNRPPPPVPPPPVIPSGQTLRVATFADYFGSRT
                     LPSFEAKTGNHIELEVFQSNESLLANLDSRRFDVVFLSGYAVEHLITTGRVAKMPRER
                     VPNLAQVPLDFRNPPYDPSLLHCVPYVWWVIGYAYLPPRTGHVIEPSSLDILFAESGP
                     EVAWLDDMRATLGMALRRLGKSANTRDPADIAAAKELLLRALPRGIELIDDPADLDKR
                     GKLSVSMGWSNDVFSLVRKVPQVRFLLPKEGTLLYVDFACVLQSARQPDVGFAFLDHL
                     LEPAISADIANTRLLPTVSEGGRKMLDTEARWLWGTLESLRSHSGSYEPLRDVGDATA
                     LYEQAWQEVKNGIEKEKARRAALPPPVPEKKPKPVPIKGKKWVNPLGL"
     gene            complement(49774..51036)
                     /locus_tag="PKL17_01360"
     CDS             complement(49774..51036)
                     /locus_tag="PKL17_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNK43399.1"
                     /translation="MSNSVTPRGDRPYAVVLFGASGFTGKLVAEYLAGLQRRDLRFAI
                     AGRNRTKLEEVRRDLVRIDPNLAELPILIADSTDKDALRKIADQTRVICTTVGPYAKY
                     GLPLVEVCAQAGTDYCDLTGEVQFVRRSIDSAHATAEQTGARIVHCCGFDSLPSDLGV
                     HLLAEHFAKSGLKLGSAKYYVVALRGGASGGTIASGLNLIAEAAADKELRRLMGDPYA
                     LYPDRQKDRGPDRGDQKGVESDDEAGYYTAPFVMAAINTRIVRRTNALSNFAYGRDFR
                     YAEVIANPKTPAGYLRSVATAAGTAAMLVVGSSDTLRGLAEKRLPAPGEGPSERARNR
                     GFFVIRLIGKSQPLPSGEVRRAECIVKGNHDPGYGETSRMLSEAALCLAEDPLPTQGG
                     VLTPAFVMGSHLPDRLRRTGMTWDVRDL"
     gene            complement(51029..51517)
                     /locus_tag="PKL17_01365"
     CDS             complement(51029..51517)
                     /locus_tag="PKL17_01365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNK43400.1"
                     /translation="MATQPTLSVGDMAPDFSLPAHDGKTYSLRELLGRRIVLYFYPRD
                     NTPGCTQQACDFRDHSARFSKTLVLGVSPDSVASHQKFSQKYALPFPLLSDAGAEVAK
                     RYGAYGEKVLYGKKTLGMIRSTFVIDESGRIAQVYGKVSVKGHVERVLADLGDALGSA
                     HV"
     gene            complement(51549..54209)
                     /locus_tag="PKL17_01370"
     CDS             complement(51549..54209)
                     /locus_tag="PKL17_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43401.1"
                     /translation="MRLRRIEVRNFRKLRGPICIDNLGDGLTVIAGDNEDGKSTLLLA
                     LKAALFLKYKATVTEDFLPFGSKVRPELRLDLEFFGRPYSLYKAFCQNPRAELVTPTG
                     KLEGMAAEEELARLLRFDSPAKANSAEPKSEHQGLCGLLWVTQGESFRNHQAASSLQR
                     DLQSALESEVGSVLTGELGRKLLQVFRDRHDENYTKTGQPRDELKKAREKVEQLAVKL
                     AGLRERQRDYEGKVDRLGDLRDKLRRYDEDGTLAKQKSALQKDEAALLLLSERERALA
                     ALQKDVTLSEERLELLRQKQKRRLDGRETVRLADEELLKLDVRKQQGLVGISTEEQLW
                     NDAVTRRKKKSDEHSAASAEVSLLRRSVDRQRLSAELFERNSRLGEVETLEKRKNELA
                     DQLVRLVVDDKQLAKLRELHRLVREREVELHAVATLLEVAVTGTAELWLDGQPVSEPA
                     TTRIVTDRTELVLPGVKLVITPGRTGAARLRESLQTEQAKLLAQLAELGVADLLTAEQ
                     EAANRRAVVDEQRELMQHLGRLCPSGVAALREEIVRLTRQLVQLGDETEPSLPQETLL
                     RQLGERQQLVESLSRELVLCDEEVERKRLLVEKLRNQQAEHDGRLSQQRREVERLKKQ
                     LDDEAAQCSDAELDVSVQAQKQVVLDKRAQAEMLENELKAADLDGIRQNVSQNKAAID
                     ELSRHLLGLTQKAQELQIELRTLGQQAIDEELAETQAAYERAQVELSHKTRRAEAIRL
                     LYESMQAHDQRSREAYLGPLLRLCEPYLQAILPGCRLAINESTLAIEGLWRGEQLEPF
                     VALSLGTREQLAIVVRLAVADLMAQRGQSVPLLLDDALTFCDDARFDRMLRLLRRGAS
                     RHQIILLTCHEREFLRSGAKLLRLVDCQAA"
     gene            complement(54213..55358)
                     /locus_tag="PKL17_01375"
     CDS             complement(54213..55358)
                     /locus_tag="PKL17_01375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNK43402.1"
                     /translation="MAVRFVHSADWQIGKVFADLVGEDPDQAALLKRERISAVERIAA
                     LARERQADCVIAAGDVFDSHTVPDDVLRRTVLAMAGYSGPWLLLPGNHDAALFESVWT
                     RLVRLGLPANVKPLLSAEPVILKDGKLAVLPAPLLRRHESIDVSDALDTMVSPVGAVR
                     VGVAHGSVKNRLPERSEATNEIADDRVQRARLDYLALGDWHGFLPIADRTYYAGTPEP
                     DRFKDADRSPSGQVLFVEMEGPGCPPQVDVVKTGHFQWEKRKLTVLSEADVGLVEAML
                     MGLAQPHRQTLLDLTLMGSLPLSAEQSLRKLLSEWRARLHLLRVHDDALLPEVSEDEL
                     DNLPAEGLLRNVVAELRQKQQQPSEAGERAKLALKLLVEEYRQAVGT"
     gene            complement(55422..55847)
                     /locus_tag="PKL17_01380"
     CDS             complement(55422..55847)
                     /locus_tag="PKL17_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNK43403.1"
                     /translation="MKLVVLAVGKLRDPWVLAGCAEYEKRLRPRLPVEVIEVKDEEQL
                     LKKLPPRYLIWVLDERGEQPSSRELSQRLDRAFHSGAPGLALCIGGADGHSAVLRDRA
                     SYLLGLSKLTLPHRLARLLVLEQIYRAQSILDGSPYHRE"
     gene            complement(55854..57155)
                     /locus_tag="PKL17_01385"
     CDS             complement(55854..57155)
                     /locus_tag="PKL17_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNK43404.1"
                     /translation="MTTPRYPCQNASDTAAPSGSDDAQRRTVLTRRVDEVESLDELFL
                     HCLPAFGGAYLRRIYTLLDAAIGAGCPLTVAIAGPVTVSGQHQTWLNPLLETGWVAYL
                     STTDAVCYHDGHRSLDGHRNPVHEVPIFGDDGALRDEGTIRVTDMAFDEQVLLDQDRF
                     LTAVLLRPEFQKKMTGTELRFLLGQYYGQQEQKNGISSGLLSTCHRLAIPILVGAPGD
                     GSVFLNAMKLWAMKQAGLIAEFGFDLDVQAEVFESCAYHYHCLFQVEPGALATLILGG
                     GVPKNYNLQPEPGLSQVLGLSEVRGYTFDVQIVSAPVTDGSLSSCPPAEAVTWGKVDK
                     DVYQRTTESMQADYSMVMPFVVKALLDNRARYARWAEKMGEAALFEKHPKARGYLRSR
                     DGYRLFDKRPAVYEQLLSAVRKNADWLKQTLSYPIAAPTAK"
     gene            complement(57203..59662)
                     /locus_tag="PKL17_01390"
     CDS             complement(57203..59662)
                     /locus_tag="PKL17_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNK43405.1"
                     /translation="MNAAPRLVCIHGHFYQPPRENPWLDTIEVQDSAFPYHDWNERIC
                     AECYEPNAYARILDDKGKITSIVSNYERVSFNFGPTLLSWMSVHARRVYDLILLADKQ
                     SMLRFSGHGSALAQVYNHAILPLCSPRDQRTQVVWGVRDFAFRFGRLPEGMWLAETAV
                     DVASLEALAKEGIRFTILAPHQAKGVRRRGESSYRDVSGGQVDTSMPYDVVLPSGRRI
                     AVFFYDGQTSRAVAFEHLLNQGDKLAERLLRGFRNGQDRAQLVHIATDGETYGHHHKY
                     GEMALAFALRTIERTDGVRLTNYGEFLAQYPPTHEVEICERTAWSCAHGIERWASDCG
                     CNSGGSPGWHQRWRTPLRTALDLLRDRLAPLYEQATATLLSDPWRARDAYIDVTIDGR
                     PEVRERFLRNQAKRPLTPDETARVFQLLEMQRHLLLMYTSCGWFFDDISGVEPLQNLL
                     YAARAIQLAQTALKVDLEPVFLDVLATAESNVAAVGNGRQLYESQVRPRKVDLAKAFA
                     HYAITSLFQRYEDPISIYGYQIEPRQQHLHRSGRLSLQLGRARVATPVTQESADLSFA
                     VLHFGDHNLVGGVVETRSEDFLGELRKNLVEPFERADLPTVLRMIDRYFGQATYSLRE
                     LFRDDQRRILDELLRNTRAEVDDELRKIYDTHAPLLRFLTDLHAPLPPALKTAAEQSL
                     QTAILAQLGAASPDFGEIRRLLDEVQRSGVRLDQAAVSFALQGLLQRLSDKLRELPDS
                     TKVLQALLDAVVLSTTPPYTVDLWRTQNAFYELQQTLYREKVQRSQQSSSESQRTFTW
                     LRLFAQLGDRLSIRLPPSTNK"
BASE COUNT        12487 a        18755 c        16811 g        11674 t
ORIGIN      
        1 ccgtatctgg atgcaacgat caaagaagtc ctgcgcctgc gtccggtgat tccgtttgtt
       61 ggcagagtcc tccagcagcc gatgcagctt ggtgggtacg atcttccgca aggaacggcg
      121 gtggccgctt gcatttacct ggcgcagcgc aatccagatg tgtatccaga ccccgatgca
      181 tttttgccag agcgattcct aaacggtcag cccgatcctg cttcatggct tccttttggc
      241 ggcggaatcc gtcgctgcat tggtgccgcg tttgcgctgt atgaaatgaa gattgtgatg
      301 ggaacgatcc ttgcgcacac cgagctggca ctggaaaatg accagcctgc cgacatcaag
      361 cgtcgcgcca tcacattctg gccggcagga ggaacccggc tgcgcaagct ctcccatcct
      421 gcgtgatcgt cgagctggcg cggctttctt tcgccacatg cacggtctat gatcacccca
      481 tgcgagagtg gggtttcatc gctggcatga tcggtgcggc agtcggtgtc gcgacagcgc
      541 tgggtgcgat cggccttatc gtgtccgacc aggccacaca cgatgcagcc gtgcttgaca
      601 cacttgcgca aagcacagtc acaagcgatc tcggcgatct gagcgatctc ggcgatccaa
      661 cctccgtcga tctggacagt ccggaccagt cttccgcccc cgatgggaat ccgcttggga
      721 gtccggttgt cgatggatca gccagcgatg gagacagtga ggattcctat tccgaatacc
      781 cggtcggttg ttttgcttgg tcgaaaaaac gcggcaccgc gctgtgtgtc gtggggaact
      841 tcaccaacga gtgcttcacc catttctggt cgtggacgct gcatgaaatt ccgggggaaa
      901 acggcttgac gaccgacatt gaaaccgtca ccccagaaga tcgcgacaaa catccagatc
      961 gcaaagagag tcgcgatcac atgcgagcac cgacaacgga tgtccaaccg actgaaaccg
     1021 atggtgagtt tgaccattcc atcaggccgc tcgcgagctt ggaaccgagg cagacgctcg
     1081 atgtactttt ggctgcggag agcggaaaac cgtcgcagtg gctgcgcttt cgctgggccc
     1141 agaccagtgc aggacggaat cggattacgc tgcagtgtct ttcgcccgga gagcttccgc
     1201 ccgatctcgc aagcggcagt gctcgcgcct acccaaccag cgaagcccca ccgctgccga
     1261 aaggaatccg caaaagcgaa gtcgatgtgt ttgcttatga aggcggaagc gaaggaaagg
     1321 tcgtcatcca ccaaaccgcg acgagcaatc gcctggtttt ggagcatacg caagagtcgt
     1381 cagcagaatg tcagcacagt gaagcccatt ctgctgcggt cgtgacgatc gggcggatct
     1441 gtacggctgc ttctgtgggt gatgccgcaa gcggactccg ggctcggtaa ggggttttca
     1501 cttcccgctt tccgctcgca aaactactcg acgctgccgt cgcggcgcag gtgaattttt
     1561 tccgcgaggt ttgattcgat ttcactgcga ccaaacagca ggtgttcctg cttcccagac
     1621 agccagttgt cgtgcgcatt cccatagtag ggactgcccg gatcgcccga attgccgcgc
     1681 gggaagttga cttgtgccgt cggtgcgccg ctgccatcaa acgtcacgat catccggaac
     1741 agcgatcctt gccgagccga ccacgagcct ttgattttcc ccgcagcatc gaccatcgaa
     1801 gacgccgaaa cattgaccgt gccgaccgaa ccatcgacgg ggaatgcgcc cgcattccac
     1861 ttcccaccca gcacatgatc aaacgttgca gagtgcacgt cctgccactg atacggcttc
     1921 atgaccggtg gcaacaccga accaaagcgc tgcttgagcc aatcggcagc gtcggcaagt
     1981 ccgcccatca gcaccagatt ccgttccgtc ccaatcagcg actctgttcc cttgaccccc
     2041 cgcagcgcca gccgcagtgg cttgagcgca tacgccggtt caaagtcgaa cagcttgggc
     2101 aacagcggtc ccagatcatc ggacagcgcg cgctgcgcca cgaagtgggt aaacgcgaaa
     2161 aagatcagcg cttccgacga gctgcgccgc atctggcgat cccacgaaga cagaagcgaa
     2221 aacagaccga taagctctgg tcgaccccgg aactgcgcaa gctgcggatc ggttccaagc
     2281 tctgccaccg actgtccaag cgccggaatc agatcatcgg ccagcgtcga tcgcgcatcc
     2341 cactgaagct ccgccaagtc tgccggcgtg atcttgccgc gggtcacaag tcgcttaagc
     2401 tctgattcaa tccgcgccgc gcgatcaccc ggatcataaa agtagccgta gtagtacgga
     2461 tcgttgtcca cgcgcccgtc ggatgtaaag ccaaacggtt cgttgttggc agtcgccaaa
     2521 tagccgcgat caggatcttt ggctgacggc agtttgtctt ccgaaaggaa ctttcccgac
     2581 cacagcgtgc gcggatcggt tccgttcatc agcttccacg gctggattcc caccgtatgg
     2641 gaattgccgc gatcgggaac ctgcgcatag accctgtagc ggatgttttt ctggtccgct
     2701 gcgacgaggt tgacggctcc cacttccagt cgcttggccg ccccttccca ttcatttagg
     2761 tttttgctgc gatccagtcc cagatacatc gccgcttcat gcgtcgcagc aagccccgtc
     2821 cacgcaaaca gaatctgctg cggcgtgatg agcgcgcgcg gaaccggcaa aagctcgtcg
     2881 ggaagcagaa caccaaagcc cggcacatcg gtgatctgat attcgcggac atcgcccacg
     2941 tcatcgctgc ttggcacttt gccgccctcg gcgcggacgc ggattttttc attgcgtagc
     3001 accgccggac gatctgtccc gccaattgtg atgcgatttc ccgacggggc cggcacttcc
     3061 cacagatcca tcacatcggc aaagttggtc gtggcagccc aggccaccga gcggttgtga
     3121 cccagctgca cgcccggcgt tccggcaaaa ccaaagccca ccacgtcgag cgatccgcct
     3181 ttgtccgcgg agtttatgtg ctgcacaaag aaacggatcg ggctcccata cggctggtgt
     3241 ggatcccccg caagaatcgg cgttcccgac gcagtcgtct tggctgcgac cgcccagttg
     3301 ttgcttccca gctcgggcga aagtcgctcg gctttttgca gcgcggcttc ggcgcgtgcg
     3361 cgcaactcgg gtgaaatcgg cggcgtggga tttcccagcg gcagcggcgc cagcattccg
     3421 gtctgcacca ttgcatttgg catcgtgaac gcattgcgcg tcggcatgca gaacggaaaa
     3481 tcggcgggga atttcggtgc cagggtcttt atcaccgacg cgagcagctc gctatccagg
     3541 ctcgatgaca ttccgaaact gagcagtttg ccaatgatgt acggatcgtc gtccgtccac
     3601 agcgtcggtt gaaaatcgag tccgtctttg cccatttccg gcggcagcgg catttcaccg
     3661 cgcctgacct gcccgatgta atggtttaca ccgcggacga acgcttcgat aagctgcgcc
     3721 tcgtcgggat agtcctgccg caaaagctca cgcgagcgct ggccccactg cgcaaacgac
     3781 atcgtgcgcg ccaaaagatc gctgcgcaga cctttttccc cgacgacttc ggactgggtt
     3841 ccacgggcct gccgccgcac cacttccagc tggaacagcc gcagcttggc catcacatat
     3901 cccgacgcaa acatcgcatc gcgatcactt gccgcgtaga tgtgcggcac acccatcttg
     3961 tcgatcaaca cttcggtatc ggccaaaagc ggagtctgaa ccgtcgtcgg atcgcaaccg
     4021 agcggcgccg cgaacaccac cgccggaagc acacgccaaa aagacaaacg agaagttttc
     4081 atgggcggaa gatagcagca gcagcgtagc cgccgtcaac accacccgcg cgaagacaac
     4141 acatgtatgg tagggtccgg ttcgtgaaag tggttcgtaa gctcttttcg cctttgcttt
     4201 ctgcgcttct gctcgcgctt ctgaccgccg cgctcctcgg ggcaagctct acctcgcagg
     4261 gatcgtcgct cgcggcggcg tcccaccctg cactttcggc gcgattttct gcttcgccgg
     4321 ttccgtcgac cccgtcgccc gacaccatgc tagcgcctgc tgcgccagcg acgacgtttt
     4381 tgggaatgga tgaggatctg ctgctcgaaa gactgcgcag cgccgaaatt cgcagcgtcc
     4441 actttagtcg cggcggcagc tcgctcaccc tgcgggtcga atttgtcgat ggcagtcgcg
     4501 ccgcgtttaa gccgcagcag gtccatccac agtcggttcc gcgcaaggaa gcggcggctt
     4561 atctgctttc gcgcaagctg ggtatttcag cggttccacc gacggtgatg cggagcttgt
     4621 cgcgcgacga acttttgcaa aagctcgatg ccgaatcgca gtttctgcgc ccgcggcttg
     4681 tgcgcgaagc gatctttgat gcgcgcggtc tgacgacggg atcgatgatg tactgggtgc
     4741 ccggacttct ggatctgggg ctcgatcggg aacgcgcgat tttgcagtgg accgctgaac
     4801 tgtcgcaaaa cggatcgctg cccaaagaac gcgaagcgat tctgtcgcag ctttcgacgc
     4861 tgcttttgtt tgatgtgatt cagaacaact ccgatcgctt ttcgggcggc aacatcttgg
     4921 gtctgcgcga cggctcgcga ctgtttttta tcgacaacgc ctttggtttt cagaccgatc
     4981 ccatcggcca caagcgcgcg ctcggatacc tgtcccgctg tcagaagttt tcgcacaccc
     5041 tgtggaccca gcttcagctt ctgcgcgacg agcagctcac gttcgacgat ccggtctacg
     5101 gtccgcttct gtccgacgag gaaatccgcg ccctacgctc ccgccgcgat cgcacgttgc
     5161 gttacctcgg tcagctcatc cacagccacg gcgtgccgtc ggtcctcgcg ttccagtagc
     5221 cgccgcttca ccccacagca tcacgccgca ggtcgtgcga acaaacgcga cctttgtatc
     5281 gtcatagcgc tacgtcttcc catccatcgc aggtcggcgg atactgatac cgaccgtttt
     5341 ttcacgcagg aggagttccc atgtcccatg ccaagctttg gttcgcggtc tgtggcggcg
     5401 ccctgattgg gcttgtctcg acgggttttt cgcactcgac cggccatgaa tcacgcatcg
     5461 ttgcgcagtg tgaacgtctg ccgacccccg agcgccagaa ctgtgttcag tgcgtcacgc
     5521 gcccagtcaa gcaccacttc caccccgact atccgcccgg cgctcgctgc cgccccgaca
     5581 acggcaagcc ctaatccaat cgaccgccgc accgacgcgc aagccgttta gcgcgcgcca
     5641 atttttagca gcacgacttt caccgtatcg atcaagatcg acagatcaaa cgccagcgac
     5701 atgttctttt ggtagtacaa gtcgtacttg agccgctcgc gcgcttcgtc gagtgtcgtc
     5761 gccgcaccga accgcacttg ccccaagctg gtgatccccg gacggatcgc atggcgctga
     5821 tcgtaaaacg gcagcgtctg ccgcagctcg ctgaccgagc tttcgacttc cggacgcgga
     5881 cccaccaggc tcatgtcacc gagcagcacg ttcaaaaact gcggcagctc gtcgatgcga
     5941 tgcaggcgaa tgaaccgtcc gacccgcgtc acccgatcgt cgttgtgcga agtcagccgc
     6001 atcggctcgc caggtttttg caaccgcatc gatcggaact tgtaaatcgt gaaaagctga
     6061 ccacgcagac cgacgcgctt ttgccgatac agcgccggcc ccgaactgtc gatccgaatg
     6121 aggagcgcga ccacaagcag aatcggcgac accagcagaa gcgcgaagag gctcagcagc
     6181 acatcgtttg cccgcttgag cgcgagcgta aaattcgacg gggtaaagct ggcatcgaac
     6241 accacgtcgg caggctgcaa aagctcgatc ggcaagcagt gcaagagcgc cgccgaaaac
     6301 gacagcgaat tccacacttg aagcccgagc agcttgacct gcaaaagccg ccgcaacagt
     6361 gcttcttcgc tgcgcggatc atcgggcgca aacaccacat cggtgatgtt gtgacgtgac
     6421 accgcatcca agatgtcggg atagctggcc agcacgggtc ttccgacgac ggtctgcgcc
     6481 atctcgggtt cctgcggtga caaaaaaccc gccagttcat agcccgcccc cggatgcgac
     6541 tggagccagc gcccgatctg ttgtcccagc cgaccgactc ccaacaccag cacgcgccgt
     6601 cgccagctgc cggattgcag ggtttcttcc agatgacagc gcagcgaaaa aagcagccac
     6661 tgcccgatgc ccaggctcat caaaagcgcc aagcgctgaa gacgaaacac cggcagcaca
     6721 tagaacaaaa gagccagcgc cccaccgccg ataagatgcg ccaaatgaat gcgcacaaga
     6781 agctgcgtcc gcgatccagt cggcgtatcc gcgtacagat ccagatagta cattgcgagc
     6841 tgaacagcga cgggaatcgt cagtccagcc agcagcgctt cacgccaata cgcccactgt
     6901 tcgacatggg gaacttcgcc aggatagccc gctccgatgc gtagacgcac gcacaggacc
     6961 gcgaccacaa acagaaggac ggtctcggcg agaaacaacc ggcgcagacg ggccgattga
     7021 aagatttccc acatgaagaa gtggtgccag tttacccgag aaacggctca gaatatcgac
     7081 agcaagcgtg gggggcagcg gcgaagcagc cgtcttggca aggacgaccg cggcagggaa
     7141 atccggtttt ttagcgcagg atttcttgga tgacctctcc atcgcggagg tatttgcgcg
     7201 gatttgcccc catcgccccc acaaacgtcg ccagcacatc gggcggcgtg accgcgcgca
     7261 ttccgtcgga aaaagccttc gccggcagcg gcagaagctg ctcttcatcg gccttgccaa
     7321 acgacatgtt gcctttgaac ttcggtgaaa tcacaagcgc cgagttgttc ggatagtgat
     7381 cgcgcccctg cgccgcgttg atcatcggcg tgcgacagaa gtctgatacc accaaaatgt
     7441 gggtgtgctc ggacagcttg ctacccatct tcgtcggatg cggaatcaca tccaggtagt
     7501 cgagcatcac cgcgagcatg tcaaacaggt tctgctgaag cagcggctgc tgacggtagt
     7561 tcgtaaagtg ggtatcgaaa cttccgtatg caaagctgac gcagcggctg acgttgcggg
     7621 ccaaagactc cagcgcaaac gccgcattga ccgacgcatt catcgtcgga tacgaaaagt
     7681 ttgggtactt ggttttcaga tacgcatcgg tgaacagatc gaccaagttt tcgcgattca
     7741 tccggcgcag accttcatat tgcaggtaca tgccgttcat cacgtcggga ttggccgaca
     7801 gaccgaccaa gtctttggct tcctggctaa gcatcgcggt caccccgtcc cgcgcctgcg
     7861 ccgactcata caccgtcgac cgccgcagcg atgccgccag cgttccaata ccggaaatcg
     7921 ccagcggtac gacccggcga tccagccctt gaccgacaaa gctcgacgga tagtttatcg
     7981 aaaccgcggg cagaagctgg gttgcgccaa acgaatccgc catcatcgtg tcgatgctcg
     8041 gcgccaccgt tttgctgccc gccaggtgtc gtcccgtcgc tgaatacgca gtcccatcct
     8101 ggtgactgac cgtattcatc gcgattccgt ttatcaccgt aagccgcgcg gcatggcgca
     8161 aaagctcgcc aatcgccgga ccgaacacga gcggtcccga cgctggacga accagctcga
     8221 acgaataacc ggacagtgca cttgtgccat caagcggtgt ggtgcgactt gtccagcgtc
     8281 ttatcggggc cggatcaatg gtgtttgtgg aagccggttc aaccagtccg attcgttcgt
     8341 tgcgcggatc gagtccgacg gtgacatccc atgcaccttg cgcatggatg aacaggaaga
     8401 actcttcgga cgccacatcg ggtccggcaa acgcacgata cagcggggtt ccgagtgccg
     8461 cagccgtgct gccaagcgtg agggtcttta caaagtcacg acggttcatg gtgccctccg
     8521 cttaatagag ctgaaactcg gggtgacgaa tcagcccctg acacaccgca gcccagcctg
     8581 cttccactgc ggaaaagcgc gagcccatcg ggttgtggtt tttcaccgtg tcgttataca
     8641 gcgcaaaaaa ccgcgccgtc cgatccgtcg gtgccaagct cgctggatag cccagaaact
     8701 tgcgatgcag tttggcaaaa cgggtcgcaa agtccgcatc ggtcagcggt gttttgggca
     8761 cgtcaaagtt gaaaatcagg cgattgtcga cggaaactgg cggcgttcct ttcaggtctt
     8821 tttccaccgc tttgtcacac agcgccacgc cgagccgttc aaagctcgcg accatcagcg
     8881 cgttggtctg ggtgttgcgc gacagatcgg ttgcatagtt tggtagaccg agcgcgccca
     8941 gataggccgt ccacgaatca aacagcgccc cgccgcccgc ctgaagctgc gcctgcgctt
     9001 gcagcgccgt aagtccacca aacagcgtca ggtacgttcg gatgtacgct tccgctggca
     9061 caagccgcgg cgtttccttg tgcgtcgtat cttgcgcaag ctggccgtga ccgggaagct
     9121 gttcgatggt ctgccccgga gaaaccggct ccgcatcttt gcaagccgac agcgcaagca
     9181 ggcaagataa gaggactttt agatgtctct tattcgtttt cattgcttgt ctccttgtcg
     9241 ccacgcggtc gagcttaggt tgttggcggc gcgatactgc tgtgatgtaa gcagtgcttt
     9301 caccagcggt ttgacccgat actttccgtc gaccagcgtc tgtgtcagcg catcccgaag
     9361 ctggctgtct tcagccccga gcgaacgacc caggaaactt tccgccatgt tctgcgccac
     9421 acacggagca aagtcggcgc gaccgaccag atagtcgcca agtccctgcg ccatcgcatc
     9481 ggcattgtct ggcgatccat acgcgccgcg cagcatgccc gcggtcggcg tcgaaaacac
     9541 gttgtcatat cgtccggtgc agtttgccgg aatgttgccc gatacctgct tacaggccgg
     9601 attctgcacc ggatactgct tcggatcgag gaagctccag tcgttttcca cccagcgcgc
     9661 aaaatacgag gagagcggct cgagtgtttg gtgacacgcc gcgcagcctt cccgtttcat
     9721 caggttcggt tctttcgatg gcggcagctg aagtccggct ggcgccacga agtcgcgaca
     9781 caaaaacatg ttgtagaccg catgcgcgcg cgctcgccgg gttccgtact tgaacagaaa
     9841 cagcggcatc gtcaggattc ctgctgccga cggacccgca tcgaccttgc gccattccaa
     9901 caggttgtgg ggcggaaagt tctgcggcat cgcatcgggc gaaggcagcg acgtcggacc
     9961 cagatcccga tcattccagt tcgatcgcga tgacagcttg taaaactgcg cgagcggtcc
    10021 gtttacgtag gtgtacttgg ccgacaccac ttcgcggaag tcacggtccc ccaaaaacag
    10081 gttgtacaaa aaaacctgcg gctcttgcgc gacccacagc gactgccagg tccggtattc
    10141 aaactgctgc gcatcggtcg gatcgtcgac accgagcagc gtattgcgcg aagaaatgaa
    10201 cgtcggtcgc ggctgcgaag caatgaacgt cgccgccggc atgcacattt ccagacccgg
    10261 accacagccg cagccttgcg agttggcgac accataaccc gtccgacagt cgagcatcat
    10321 cgtcggctgg gtgtccatcg ccgcgctctg ggcgatgccg gcttcttctt tgcagacacg
    10381 aacttcggtg gtgtcggtct ttagatccga ttccttgatc agatcatttg tgatcgcata
    10441 ccccggaaag cgcgtcgccc acgtcgcgga gttatagcga tcgctcggat ttgtgctctt
    10501 gtaatcggca tacagccacc acggctttac aacgaccgtg tattgatcga gcaccgcttt
    10561 gtcgattggc ttggcggttc ccgcaggcgg ctgcgcacca cttatgtccg gatatttcag
    10621 tccggtctcg tcttccagaa gacagaagcc gccgcgcacc aagttgttcc acttgggaaa
    10681 gtttggatcg gacgctttgg gcacttgggc atgccgcgct cgccgctgac cgggacggaa
    10741 atagacccag acatctttgc cagcgtcgtc ttttaccaaa atccgcgcca gcgtatttcg
    10801 tgacgtaccg acctgaaatg aaaccgtctg cggacgcagc agatcggaat agacccgcgt
    10861 cagccgatcc gcataggtgt cgccttgcag atgtgcatcg agccacgcat cgagcgaaaa
    10921 gtcggctttt tcaaacgctg ccagatcctg ttcggtcggc atccgcccga gcagatcgat
    10981 gcacagcgag cgaaagtact tgtaatcgag cagcgtcttt tgcggttgcg ccgcttggga
    11041 atgcccgccc cacagcgcaa gccccagcgt cagcgtcagg agcggctttc ggaagcgacc
    11101 attccgtcga gaaaacgaaa ctcttgcgtc tcgcatcgat cctccaattg tgagcaaaac
    11161 ttcacaacga gagcgcgcgc ggtgcaagca atttttcggt tacgtttttg cggacaaacg
    11221 gcccgacaac ttggcacgcg gcgtcgccga agcctttcca tcggcaggct ttttcgcctt
    11281 ggcagatttt ggcggctcgg cagctttcgc ttctttcaga actttcggaa ctttcggaac
    11341 gtttgaaact ttcgaagtct tcggaacttt cgcaaccttg ggcttgggat cgggcagctc
    11401 gcgcgcggtg atgcgaatca gctcgcacag ccactcgctg tcgtcaaagc gatcccccga
    11461 aatcagaaac gacgccttcg cccccggata ggcaaaaccc tcttccggag tgccgataaa
    11521 acgacgcccg ccttcggtcg gtttcacaaa cagctgatca tcacagacca gcgcgacaat
    11581 tttcccgtcg aggtagacgc caaactcacc gaacatccgc ttggcggtca tcggtcccgc
    11641 caaagccatc tgatcgagca aatagtcgac cgtgctctgt tttgaagcca tcggggtctg
    11701 tctcctttgc gcagcttgtg tagcaaaaat cgcagccaga tgacgctggt ttttcgcgtg
    11761 cgaaaaggag agcgaacgct acttgggaat cgcttgggaa tcggcggact ttggcaagtc
    11821 ggtacgtggg agacagtcag aaaccaagct gtccaagaca ggtccgcagg tctggcccga
    11881 gcttgcacaa tcgacgctgg caagcgatcc gtcataacag aaagacagca cggtattgtc
    11941 gcagcgaccg gcctgcgtga catccccgca cggcatgcgg gaagcgggct gcggacagtc
    12001 ggcacagctt ccagattcgc tgatgtccgg atgcgcgcgg aacagctctt cgagcgtcag
    12061 tttttcgtcg tgaacttttt tggccagctc tttgccgaca taccgaatgt gccacggctc
    12121 gggcctgtag ccggtcagtc gctgcttctg cgctggataa ctttggacaa aaccaaactc
    12181 cgcagcgtgg tttgaaagcc acgcgatggc cgcgccggtc ggaatccgca gatcgatcac
    12241 actgccgagc tggtgttcac tgtgccccgg acgcgccgct cgcccgatct ctttgatggt
    12301 cgcaaacacc cgcgcttgat cttcatagga acggtacgcc gactcgactt tcagggcgtg
    12361 accttgcgcg cgcgattcca caagcagcgc ttccagcggc tcacgcacgt cgcgaagcac
    12421 gcgatgtcgg cctttttcat ctggctctgc acgatcattt atccggacaa gattcggccc
    12481 cggatcaaac gaacagaccg gatgacagcg ggctggattg acccacggaa gccgcggtgc
    12541 tccgttggga agcagagacg atccagcttc cttttcgccc ccggtgattc ctttttgatc
    12601 cggtttggtt cccagtcccg ttgtttccgg attcccaagt ctgccaggcg aagacaggga
    12661 aacggattcc gaagtgggtg cctgctgcgg aaccggcaaa ggtgtcggct tcaccgtcga
    12721 cacacaaccc gagacaagca gcggaaacaa agcgtagcgc ccgatccgca ggaccaaaga
    12781 cagacggcgg gccggcgcta ccgattcaca aggagaagca aaccacacgg tatccacagc
    12841 cagcagtatc gtcgaaaatg cgaccaaaag ggagtccgaa acgatcgcaa gtcacagctc
    12901 gttccagccc gaaacgtccg cttcgctgag cggcagctcc agcttttcaa actcgctgcg
    12961 cgtcgcaaaa agcaggtgtc gcatcgtcac tggcaggcca tcttcggcgg ccaaaaatgc
    13021 cgcggccatc gcgatgttgc ggatgtgtcc ccccgcgatg gacagcttcg ccagccgatc
    13081 gaaatcgacg cctctttgcg gaacagaatc gggcagcgcc agcttccaca gtgacactcg
    13141 ctggcttcgg tccggaaacg gaaactgcac cacaaaacgc agccgccgta gaaacgcagc
    13201 gtcgagcgag gagcgcatgt tgctggtcag aatcgacacc cctttatacg actccatgcg
    13261 ctgaagcaga tagctgactt cgatgttggc gtacctgtcg cggctgtctt tgacttccga
    13321 tcgcttgcca aacagcgcgt ccgcctcgtc gaacaggagc accgcaccgc cctgctctgc
    13381 cgcatcaaaa atccgccgca gattcttttc tgtttcgcca atgtacttgc tgaccacgcg
    13441 cgccagatca atgcgaaaca gatccagacc cagcgcgccc gccaccactt ccgccgccag
    13501 cgtcttgccc gtgccgctct gtcccacgaa cagcgccgtg acccccagac cgcgacttga
    13561 gtggcggccc agaccccagc ggtgatacac cgtcgcccga tgtcgctgct gtgccgtgag
    13621 cgcgcgcagc atgcgcaggt gggaatcggg caagatcaga tcgtcccacg tcgctttcgg
    13681 cggcaggact tccgccagct cgtcaagctg cggtcgcgca cgctcacggc acgcctgcca
    13741 caaggcatct tgcagaagtg cgtccggctg tgtgtcgcca ggaatcgacg tcttcagccg
    13801 ctgcgcatgc cgcaccgcca gatcgatgtc ctgggtatcc agccgaaaac agctcgccag
    13861 ttgttccaag tacgcaagtc gggccgcgtc atcagccccc agactttccg cctccgcatg
    13921 ctgcgtgagt gccgcttgaa acagatccac ttgttccaaa aacgtaggcg ccggcaccgc
    13981 aatccgcagc accggacgct gaccgattga cacaagctcg cggctgctga ccaccacaac
    14041 acctgggata cgatccagaa acgccgccgc cgcccgctgc acgtcgggcc cgtcttgttc
    14101 atcaagcagc agcaaaagtc ctgccggttc cagcagctgt tcgcgcgtcc acagaccgac
    14161 aagctcgtca cgctcacgcg ggcgactggg aagatccgcc gcatcgagca gaaacatcgg
    14221 cagacccagc gactgaccca gctctgccgc caccgcgcgc cgcgccgctt catcgggccc
    14281 gcacagccac agcacctggc ccccttccgt tcccgcttga tcggaaagca gggtttccat
    14341 ggtcttgaga agcggctgct gagacggaaa cgtccgcggc acatcccgca gcgggcgcaa
    14401 aagatgacgc agtctttcat ccagcgtgtg cagacccaac aggtagtgca gcaccctgtc
    14461 ttcgatccgc agcgtcgagt ccgcaagtgc cggccccggc acaaagcgca aaagctgggc
    14521 ccggcgcaag attccttcgg gcaaaagcgc ttccaggtca gcgtcggcag ttagtcccga
    14581 aagcaccgcc aggctgcacg ccgcaagtcc gtggtttgac ttggccagct gcgcgtagat
    14641 tccgctttct agctcgaccc ctgcgcaaac cagcagcaca tcgcgttcaa aatcggacag
    14701 ctcataaatc tcgcacagct caacaagtcg aaacggcggg ctcgcattgt ttgtttgcga
    14761 atccgctgtc tcgaccggca attgcagatt taccgcttcg ctcgccgagg agtcgctcgg
    14821 aaccggattc tgacccgtcg attccgccaa ccgcgcaaac gccgccacaa cagagctgcg
    14881 cacccgcgca agctccgcgc gcaaatggcg aaaattttcc gaaacccaca cacggcgttt
    14941 tctcgtcatc gcagatccag ttcatttcag acgcacttgt tatgtctcat cattttcttc
    15001 ttcaaattgc acttgcccaa gcgggcgaaa ctctcgatac acggtttttc cgctcattct
    15061 taaataatcg cgaagcgttc cttcatcata attgcgccca accttccggg tcagttgata
    15121 atttctcctg ccgagaacac gacttggcgt ttgcactttg ttcacatact catcaaacga
    15181 tcgagcctgc ttgactctgt caatatctcg ctgacgtgag ctgcggtcag cccattgatg
    15241 attccatcgt atttggtggc ttgctttgat attagaaatt cttttatcac gaattaaccg
    15301 tgtatcaatt tcatcatcaa gcgtgcgctg aggattcgct tgccggtcag ctttcttgtt
    15361 gctaaaatcc aactttgccg gtcttagaag gtttacacat tcttccgcag cagccatcag
    15421 aagcgtcagc attccattgc gatcaagaat aatctcctga gcaaataggt tatcgagtgc
    15481 agtttggaga gcgaacggca agtttatctg cagtcctgca tttttatgta tatatgattt
    15541 atataaatac tttgcatgtt caagaaaccg accatccgtg ctggcaagaa aggaatagaa
    15601 ttcccgaaca aaatagtagt tctctgttac aaattgaccc cggcagtgaa gctcaaagtc
    15661 cagcgagtct ttgcgctcct ctcgactcga ccccggacgg aagcgtgcca ggatcaactt
    15721 cacgatctct gattgctcca tctgccgctt cagcgtcgat tcatccacgt tttccgcaac
    15781 atcgccaccc tgcatcaatg cgttcgcgct ggcgggggtc tgtgtcggtg ccgcggatgc
    15841 aacatcggtc tgggtctgct gggaattggc tggtgcgcgc cgtcggagct tgtcaaaaaa
    15901 acttggcata cagagtcccc catcgccgca caaaggtcgg ggtcgaaagg cggctacctt
    15961 gaggatagga gcggtgacac tgacggggca gtccaaaaaa cggaccgcag ctttcgcttt
    16021 tgccggaaaa agacgctaat cgctgtcgcg atcttttttt cgcttgggat aaagctcggc
    16081 catcacagcc gagtcggtgt cgccccacca cggaccttcg cccgcctctg tgccgcgctt
    16141 tccatttttg cgctgggcat tcccacgctt ggccgccgac agccacgggg tcttgccgcc
    16201 ttcctctgcc acccccggat cgatgtcact gatgaacgga atcggaacgt cgtactttgc
    16261 cgactgaagc cgccacgagt tgcgaaaaaa atcgagcatc agatgatagc agtccgacgt
    16321 ctcgacatac aggatcggtg gcgtgagcgg ctgaagtaca gacacggtct gcgccacgtc
    16381 cacgtagttt gaggggtcgc caccaaagtt ccagcacgcc gcaaaattca ccggctccgg
    16441 cgactgttca gacaacacct gaatgcggcc cggctcgacg acaaactgcg ctgcatccag
    16501 aaaaatatcc tgtcccataa ggacaatcgg cgcggcaaac acccgctttt tggcatagct
    16561 ctgcgcgcgg tcgtaaaact cgatgatgtc ttcgcagcgc gtcgcatcaa agcgcgagat
    16621 cgaatggaac ggacagcagc atgctcccgc ctcttctggg ggtggaaaca gactcccttt
    16681 gtcgagcgtt ccgaacaccg gctctgcggg cttttgccac gtatgaagaa gcttcggaat
    16741 ccactccgtt tcactcgctc ggcgcggcag cacttcggtc cggcagagct tgtgaaacgc
    16801 ggtcccagaa atcgtgcagt tgaagctatc gagtagcgca atcgcagcgc actcttgcaa
    16861 cgtcaagtgg cccttggcaa accagaagtc gtaaccactt gcaacgaagt gaaccccttt
    16921 gaattccttg tgaacatact gggtttcggc gttccgttcc tcgtcggaaa gaccagcaaa
    16981 atgcgcccgc gcgacgcggt tgtacaagtc atcggcaaac cgacttagct cgggagcaaa
    17041 gacactctct ccggtcagat aacaggcctt cgcaaacagc tcgccgatct tgtcgtcttt
    17101 tgcgaccttg ccgcagtcat acacaagctc tgttcgcaaa aagaatttct gtccgtcttc
    17161 gtagggggcc ccttcgtgcg caaacgactg atcaaacagc acgcatgtca tcgcatcgat
    17221 ctgctccagc tcgacatcat cgccgatccg cagcgtcggt tttccccgtc cagaagtcag
    17281 gtacaaaagc agcgtatagc gcgagtgctg cttctgcgcc gcatcgtaat agggggaatc
    17341 atagtgaagg tggaactttc gatcgccagg tgcaaagcgg tttgcccgga agaccggatt
    17401 gacatgcgaa aaacccttca gcagcgactt cggcagcgca ctccgcagcg cagatgtcag
    17461 gacttcggaa agccgcgccg cgtggaaaat gaagcgatcg ccaccccgcg atccactgtt
    17521 gagcggtgct acatacagac ccagttcatc gaggctgagc agtcgtttct gggtgtcttc
    17581 cgagacctgc aaacggaacg aatagcgatc cccttttttg gcaagccgct tccgatcgtg
    17641 aagtgtcgca tccagtgagc cgtgcgaaaa gtagcgatcg agcggcagga tgttttcata
    17701 ggtccgatag gacagctctt ccggatcgac aaacaccgcg cacagatgcg gaagctgggc
    17761 cgatggtctg gcaaacttgt cgcgcagcgc gagcggaatc ttttcctcgt cgacaaagcg
    17821 cacaagcttg gcgcccgcgg tcggatgaaa atagatagac agtccttcca gtaccagcgt
    17881 gggcggcggt gaccatgcgg ccacgatcgc acggaactgc gcatcgccac ctgacgcgcg
    17941 attggcaaca gacagcgcaa agtacgtgtc acgcagccag cagccaagag catgggcatc
    18001 gccatccgaa agtgtcttta ccgactctgg atgttccgcg acaaaagccc attttgtttc
    18061 gggattggcg gtctgattct ggttcatggc tgcccccaca tccgtcgcag tacaagcggc
    18121 aactgcgaag acttggccag cgccaagacc cccgcttttt tgatttcatc gcgatacaga
    18181 tgaagctgcg aaagctcgcg aacaatcggt gaatttgcca gtgccacggc ccctttgctg
    18241 ccgatcttgt tgtagcgaag atcgagcagc cgcagctccc gcaggtgcgg actcttggac
    18301 agatgttgga ctcccttggc tgtgatggcg cagcgctgaa gccacaaaga acgcagaccg
    18361 gcacactgcg gtgattcagc cagcgtctgc gcgcaagcgt ccccaacata ggaatagcgc
    18421 agatccaaag acagaagcgg tcccatctgc gatgaagaga gcagtcgagt cgccccctct
    18481 gccccgatca gacaatatgg catcgccagc acacgcagac tgcgcagatc cagtgcggcc
    18541 agcgctttgg cttctgccag cagcgttggc tccgggggcg tgttgacgtc ttccccttcg
    18601 ccggaccact cttcctcatc ctcttcttct ggaaggccat tttttgtcgg cagaaagcga
    18661 agcgaccgga acagaaccgc atctcgtgtt tgcagaaaat catgcagagc cgaaaccgca
    18721 gctgtggtca gtcgaagctg cagtccgacg gcaaagccat acttccagtc tatttttgca
    18781 tcgcgtggca gaccgcttat ctggggcgtc gcttcggcat acaagcggtc gagctctgcc
    18841 gaaagcgctg caaatcgctc gctgcccgct ggcgcaaacc cccgtttttg cgacagccga
    18901 atgatccgcc cgcgcagatc accttgcgcc gtcaaatgct cactgtacct atcccacgcg
    18961 gacagatcgg aaggagcccc ccgcagccgc gactcgatcg cctgatggtc catggcgcat
    19021 aagcgtaccc tattttggcg ccacgttcga cccggctttt gcgatcattt ttccgacgga
    19081 aattccatca gcgcatcgcg cagcgccagc caccgaatgc gccttccagc acttgcgccg
    19141 cggccagcgt cagatggtca ttcccttgcc gcgcgatcac ctgcaccgcg accggaattc
    19201 cttcttcatc aaagccaatc ggaaccaccg tcactgcgta accgagcacg ttgaacagcg
    19261 ccgtacattc ggcatcaaac ggtgtcaaaa gcgccagctt gtgacgggga gcgggccgac
    19321 tgtagggtgg atgcagaatc acaccactgt ccccaagctg cgcatccagc gattcccgca
    19381 gccgcgcaag ccgcagaagg agtgcatcaa aaccaagcgg cagcttgcgc agaatcgact
    19441 cgcttagcat cgtgatcagc gtcggtagca cgaagtttga acgaccaaac ggataccgca
    19501 acagctcgcg cagaatcgga attccgcctt cccctgacac caccgattca tagccttcgg
    19561 gattggcggc ttgcagcgca ctggcccaca acataaaacc gccatccaga tcgcgcgctg
    19621 acagcacgcc ttctttggcc cccgcttgca acagtgcgcg cgccgacaga tcgatcgctt
    19681 cgcgcatcac cggacggatc ggcgaaccac catgccaagc aaagacgcgc agcgacggaa
    19741 tgtggacact ctgaggatcg cgtagcggca tgcgttgaca gactggatcg tgatcgtccg
    19801 gtccagcaat cacgcgaaga atcggcatca catctgcgac ccggcgacac aatggaccaa
    19861 tcaccatgta gccgtgtgga tctttggctg cgggaaaatg tccggtgtta ggaacaagcc
    19921 ctgccgatgg cttgtgtccg acggtgccac agaacgccgc aggaatccga atcgaacccc
    19981 cgatgtctga tccaatccca aacggagtcg ctccgcttcc gaccagagcg ccctcgccgc
    20041 ccgaagatcc gccgcacgtc ctgcggacat cccacggatt gctggtgcgg ccccagatca
    20101 ggttgtgggt ttccatccac agaccgcctt ctggaacatt ggtgacgccc aaaggaatcg
    20161 caccggctcg acgcagccgc gcgctcacgg tcgcatcttg ggtcgcgacg actccctttc
    20221 ggtgcaggac accgccggta tgcggcattc cttttagcgc gacaaattcc ttgatcgaac
    20281 acggaatgcc caaaagcggc ggcagcgtgg tgtgatctgc cgcatcatac agtgcatcgg
    20341 cagcatcggc ttccgtgcag gcctgggcaa accgatcaac caccagtgcg tttagcttgg
    20401 gattgacttc cttggtgcgc gcaatgtgaa cttcgacaag ctcgcgacag gtcagcgtgc
    20461 gggcccgcag gcgggtcaaa agttccccca cggaaagatc aatcagcgac ggactttctg
    20521 tctgcggata gcgcttgtgt gaagtttgaa gtgcgtgcat aaggagccct tttctgcgct
    20581 taaggagccg gcagattggc aagccgggcg acggtttcga tcatcacttt ggcgatctcc
    20641 gcagcaaacg gaatgtccaa gttttcgacc gtatcgctcg actgatgatt gtggggactc
    20701 tgctggtctg cgcggcggta gtcgatgtgt tcgttaaaca gcagacccgg aaatcccaaa
    20761 aactcgaaca cctgcacatc ggtctgatac accgcactgc gcacggtgtg acgcggcaca
    20821 tagtgactgc gcagcgacgg tgcaagcttt gccgatgcat ccagtgcaag cgccgcatgc
    20881 tgggccgaca aaggatgtga agtggcgttt atctgaaacg atcgatcacc ggcaggatgc
    20941 cagccgatga aatcggagat caacagcgcg cgaacttcca cgcgatcggc aagcagtttt
    21001 tcagcaaacc gccacgcccc cagatcatcg gaaggaaact cttccccggt caagtgcaca
    21061 agccagacat cggcgttcct gggcagtgta cgcagtgcta ccgcggcccg caaaagaaca
    21121 gccgtggccg tcacattgtc atcggccccc ggagtggtca cgcgctggcc ggttttttca
    21181 aacgtatctt cttccaccgc gctgtcgatg tgatcggcca agatcacttt gggaagtggt
    21241 tttccgtcgg cacgcgggat tccgggcagc ttggcaatca ggttgctctg cgcgatgttg
    21301 cgaaacagaa accgctgccg ttcggtctga attccgagcc gcgcatagcg ctgttccagg
    21361 tagtcgacca tgtgttccag atcatggtcc ggctgaaacg atcctttttt gacaaagcga
    21421 cggctgacac cggactttgg caaaaccacc ggatcgaccc cggaaaaaat ccgcaggtct
    21481 ttacggaact gcgcctcgaa gtccacggga taggacgcaa tccgcagtgg ctcgtcgatt
    21541 ttcccaggcg acacaggcgg attttgccaa tcgatcgcag gccaatcgag cttgccattt
    21601 tccacagcca gcaccatcgc cagcggttca cttccatgac gcgccggctg attcatgcag
    21661 tacgccagat aacgacccgg cgtccgcagc gtggcttcat gccgcgattg ggaatccgga
    21721 tgcggccact tgagcaccac ctgcgaggtc gatcgatcga cgtagacaca gttgccaaac
    21781 tgcggctgaa aatacaggtc gctcgattgc agaaagtcat caaacagcgt cgacgcatca
    21841 tcgggctggc tgggaacata gcgcgtaggc agaagcggct ttgtccccac ttcctgattc
    21901 caaaacggca gccagttttc gccatctacc aaaaaacgcc acggctcttt gggactcgtt
    21961 cgatagcgcg catagaccca gtttggctgt ccgttccagt cttgggtggg ccagatcaga
    22021 agctcgaccc ggcgactgcc cagcgtctgc cgaatcaggt ctgcctgctt gtccgccagc
    22081 ttgcgcagct gcgcaggaag tcgaagaacg gagtccggtt tctgaccgtt tacaagctga
    22141 accggagtcg cccccacaaa gatcgggaag tactgacgcg aggtatctgg aacggaaagc
    22201 cacggctttt ccagaagtcc cccaagcagc tctttgtccg tcggcagagt cgacagcgcg
    22261 ctgtccgcat gcgacgcaag gaccggaacc caagacagtc ccacaacaca gcagatgcag
    22321 acccgcgtgc gctgccgcat cggctagcca cccgacttct gcgattccgc ttcggcccga
    22381 tagatcgcgc acagctcgat gtagtgatcg gcggaaaact cgatccagct tagctcgtct
    22441 tggctcagcg gacgcttgat ccgacccggt gagccaacga ccaagtgacc cggaggaatc
    22501 tttgtaccgg gggttaggag tgaccccgcc ccgatgatgc agtgatcgcc gacctctact
    22561 tgatcaagaa tcgtcgatcc cattccgatg atgcagtggc tgccaatggt gcagccatga
    22621 atcgtagcgg aatgtccgac ggtcacgcgg tcgccgatct cggcaggaaa gcgcccatgg
    22681 gtgacatgga caacggtgtt gtcttgtatc gaagtctcgc tgccgacccg gatgtgatgg
    22741 acatctccgc gcagcacggc gccgtaccaa atagtggaac gctcgccgat gtgaacatcg
    22801 cccaccagcg tggcgttgtc ggctacgaag acactctcat gaacgagcgg agagattccg
    22861 cgaaagctgc gaatgattgg catgtgacag gatggatttt ttcgccaccc tatcacgccc
    22921 gcaaaaagac cgcgaaaatt cccacggcag ctacagcgct gaagtgctgg gtgcagcgac
    22981 gggctcggga agctgcgcaa gcgacgaaaa cgcaacctgt ccctggcaga gtgtcagcac
    23041 cggccagccg cgcagcggac gatgcagaaa cggggtgttg cgactgcggc tgcgcagctc
    23101 ttcaacccgc acgacacgct cggttttgag atcaagcagc accaagtccg ctggtgcgga
    23161 gaccgcaagc ttcggaatcg gtgaagacag aatccgcgca ggaccgcagg ttagcagttc
    23221 gacaagacgt tcaagcgaca gcagattgcg cagcaccagc tctgtataca gaagcggaaa
    23281 cgcggtctcc agtccgatca ccccaaacgg cgctgccgca agtccgcgtg ctttttccgc
    23341 tggagtatgc ggtgcatgat cagtcgcaat cgcatcgaca gtcccatcac agagtccagc
    23401 caagagtgcc gcgcgatcgg actctgtacg cagcggcgga ttcatcttga agttgccatc
    23461 atcagaagca atgtccgaat ccgaaagcag caggtggtgc ggcgtcactt cacagctcac
    23521 ccgcagtccc cgcgctttcg cactgcgtac aagctccaga ctctgcgcgc agctcaagtg
    23581 acagacgtgg tagtgggcac cgctcttttc ggacagcaca atgtcgcgcg ccacatgcac
    23641 cgcttccgct tcggaaggaa tcccgacaag acagcgatcg cgcgctgtgg ctccttcatg
    23701 aatcaccccc aggtgcgaaa gcgattcatc ttcacagtgc gcaaagatcg gcagaccgac
    23761 ttccgccgca cgcagaagcg cttgccgcat caccgcagcg cgctgaatcc cgcgtccgtc
    23821 gtcggacagt gcaacggccc ccgcactcct cagtgctgca aaatcggtca ggacatcgcc
    23881 tgcgcttccc atcgtcaccg cagcaatcac atcgaccgca accaccgcat cggtccacag
    23941 acgcttcctg agcgattgga gctgctccgc ggaatcgagc acaggtcggg tgttgggcat
    24001 gcacgcgatg cgcgcaaaac cgccgcgcgc cgcagcccgc gtaccactac cgatcgtctc
    24061 cttgtgtgac tgtccgggct cgcgcaagtg aacatggatg tcgtgaaagc caggggccaa
    24121 aaacagcccc gaagcatcca ccaccacatc agaaggcgaa gcagaaaggg aatcggcagg
    24181 agccatcgcc gcgatcagcc gatcttccac aagcaggtca atttggcttt gttttccgtc
    24241 gacaaatgcc tgcgcaccac gaaacagaat ccgcttcact tgagcacccg cgccagccat
    24301 tcaagcaccg ccatgcgaat gtacagaccg tttttcacct gctccaaaat cagcgagcgc
    24361 ggatcttcga caagctctgc ggccagctcg acgccgcgat tgactggacc cggatgcagc
    24421 aacacacagt ccggatgtgt ccgcgcaaag cgctcttccg taagtccata ctcttgcaga
    24481 tagtcgatcg gctgatcgct ctttcctgcg tccggaacat ggcgttcgcg ctgaatgcgc
    24541 agacacatga tcgcatcggc actttccagt acatcgtcaa gcgctgcgat ccgtacttcg
    24601 gtcagctgcg gaatgtcatc cagcgacggc aaaaactgcg gcggaccaca caggataagc
    24661 cgcgctccca tgcgctgaag catccgcaga ttggaacgcg caacccggct gtgcacgatg
    24721 tccccgcaca gcacgatggt cttgtcttgc aagcagccaa gtcgacgctc cagcgtgaac
    24781 ccatccagca gcgcttgcgt cggatgcgcc atcacgccgt gacctgcgtt caaaagcgag
    24841 atgtccgatc gaccacacag tctttcgtac acattggaat ccggatggcg caagatcgct
    24901 atcgtcacgc ccagtgccac caatgtctgg agcgtgtcgt acagcgcttc tcctttttcg
    24961 atgcttgatc gactgacttc cagcggtatc gccgccgcac caagtcgctg cgccgccagt
    25021 tcaaacgaca agcgggtgcg cgtgctcggt tcaaaaaaca gcatcgccgc aatgtgtccg
    25081 cgcagtgtct gcaccgtcat gttgccaagc ggtgtcgacg cttcacgcaa cgccgccgca
    25141 cgacggaaaa ttcgatcgat gtcgcggttg ctcagcgcat ccacatcgat caagttgcgc
    25201 gccggctgcg ttccttccgg atcaactggc gtggccagcg tgcgatccgg tacgcgaaac
    25261 ggagtcgtat ccttttgtaa ctctgcctgc atcaggtggt ttccttacag cttcggatcg
    25321 atcgtttggg tcaagatttg gtatgcatgc agcacgcgat cacgcgcctt tgtgggcgtc
    25381 tctcccagcg cagacagatg ccccattttc cgccccggtt ttggcttggc tttttcatac
    25441 aggtgaaggc gcagcgtcgg aacatcgagc atctgcgcaa catcgagagg acgatctgca
    25501 agccacaaat cgcccagcag gttgacgatc gctgccggct tcacaagcga tacgtctccc
    25561 agcggaagat cacagactgc ccgcacatgg gcttcaaact ggctgacaca cgtcgcgcgc
    25621 tctgtggtgt gaaacgtatt gtgcggtcgc accgccagct cgtttacaag aagcgctccc
    25681 tcgcgattca caaacagctc caccgtcagg agtccttcca ctgcaagctg ttcggcaatc
    25741 gatcgcgcaa gctcttgtgc ctgctcggag agccgaaacg gaatctcctc ttgcggcgcg
    25801 ggcaggacac tccagcgcaa aattccgccc acatggtgat tgtatgcaac cgggaacacc
    25861 gcgatgtcac cacggggacg gcgcgcgacc aataccgaaa gctctgcggc aagcggaatc
    25921 gcttgctcga ccaccagcgt ccctggtccc agcttgtcaa agacctgggc tgcatctgcc
    25981 gctgttccac agcgaagctg tccgcgccca tcgtatccgc cttgactccg cttgacaaaa
    26041 cagtctggga aaaacgtctg cagcgcgtcc gccagcgcag ccacatcaga gaccacttga
    26101 aacggagcgc acggaaagcc gctacgtgaa agccacttct tttgcagcgc gcgatccgac
    26161 accaaagaca gtgcacttat ccccggacgc agtggtgcat aggaatccgc agcgcgcagt
    26221 gcatcgctcg gaatgctttc gatttcaacg gtcacaacat ccgatccacg cgcgagtccg
    26281 gctgccgctc gcgcatcgcc aaagtccgca cagatgcacg tatcgacgac aaaacgcgcc
    26341 ggacaggccg gttctggatc aagcacttgc acgcgatagc catagctgcg tgccgccatc
    26401 gcggtcattc gacccagctg accacccccc agaattccga tgaccgcgcc cggcaagatc
    26461 gctccgctgc gcttcatcac gcgctccctt gcgacggatt cagactgtct gtgactgcca
    26521 aaacacgatc gcgctgggaa ttccttacgc gatgcagtct gtcgcgcaga ctgggcctgc
    26581 taagcgcgca aatttccgct gccaaaagcg cggcattggc ggctccgggt gcaccaatcg
    26641 ccagggttcc cacgggaact ccgcgcggca tctgaacgat ggaaagcagt gcatccattc
    26701 cttgcagcgc agtggccgca atcggtacgc ccagcaccgg caccgtcgtt agcgatgcaa
    26761 ccattccggg caagtgcgct gcacctcctg cggctgcgat gatcacttcc agaccgcgcg
    26821 actcggcttc cttggcaaac tgaaacatcc actctggcgt gcgatgcgcc gacaccactc
    26881 gcacttcatg cggaatctcc agctcgcgca gcagtgccgt ggcttcgctc atcacagaat
    26941 ggtcggaagt gcttcccatg atcaccgaaa cccagggatt ttccgtttca cgaccgccga
    27001 tgtcgcggcg atactgcatg ccggaaaagc gcaccgccga aacccctgca taggccgccc
    27061 gccgcgccag acaaggagtc ttgccctgcc ccaccacggt caggacacga ccgcctgcgg
    27121 tggtaagcac tccgttctgt ttctgtgtcc caccataaac aagagaggca ccggactttt
    27181 gaagtgcatc cgccacgatt gtgatttcat cgcctgcgcg tgcgacctcc ggataacccg
    27241 atgccgccag cacaaccgcg cacgtcgcat ccggcagctg attcagcaca aagtgttcaa
    27301 gctgaccacg gaccatcgct tcacacagac gcagaaactc ccctttgcca agccgcggca
    27361 ggatcgcttg cgcttccgga tcacccagcc gcacgttgca ttccaagagc ttcggaccgt
    27421 ctgcagtcat catcaaaccg acaaacagaa aaccaacaaa cggacatcct ttctgacgaa
    27481 gcgcacgcag cacgggcaac acgaactcgc gctgcacttt gtctacgccg tctgcatcga
    27541 tcgcaggatt gggactgacc gcgcccattc ctccggtgtt cggaccttca tcggaatcac
    27601 ccagtcgctt gtgatcgcgc gcatggggaa gcgccaccgc ggtctcgtga tgacaagcaa
    27661 aaaacagcga cacctcgacg ccgcagagcc gttcctcgat cagaatctgt ctgccggcct
    27721 cccccaactt tccttccagc agcgccgctg caacctgtct acattcatca aaatcatcgg
    27781 gaagaaagac tccctttccg gcagccagac catcggcttt gaccacgggc ggactggcaa
    27841 aacgaccgag cgcagctgac agctcggaaa ccgagcgaac caccacacac tgtggagcag
    27901 gaatccgcag cggtgcggta agctcgcgca taaagacctt ggacgattcc agccgggctg
    27961 ccgcttgact tggacccacg cagggaatgg cgacgcgggc cagctcgcct gcaattccca
    28021 acgaaagcgt cgcttccgga cccggaatca ccagatcgat cgcgtgggct tttgccatgg
    28081 ccgcaagcgc cgcaacatcg gtagccgatg catccaggtt ggttgccaga ctcgctgtgc
    28141 cgggattccc tggcgcgcaa aacagcgtca cgtcttcgca ctgcgcaagc gccacaatca
    28201 ttgcgtgctc gcggcctccg ctccctatca ccaggacacg tcgtttggga gtgttcatga
    28261 aggggactcc taatgacgaa agtgccgccg accgctaaag accagcgtga tgttgtgacg
    28321 atcacaaact gcgatggttt cggcatcgcg cactgatcca cccggctgga tgatcgctgc
    28381 gattccggca gctgccgccg cttctgcaca atccggaaac ggaaaaaatg catctgaccc
    28441 aaggaatgcg ccgcgcgctt cttttgcgga tttgtgcagg gccagcgtcg ctgcatcgac
    28501 ccgactgacg tgcccggttc ccgccccaag cagcgtcagt cctttgctga cactgatcgc
    28561 gttcgagcgc agcgtcatcg ctgcatactc tgcaaactgc agctccgcag tccacgcagg
    28621 atcaagcgga cgccggctca ccacttgcca gcttgccgga tcgctcacac cggaatcgac
    28681 cgcttgaagc agaagacctc ccccgatccg atgaaaggac acgcgcggct gcggatctga
    28741 agcgggcaaa accagaagtc gcagattggt tttttccgcc agaagtgcgc gggccccgac
    28801 ggaaaaacca ggtgcgcaga tgcactcgat gaacagcttg ctgtcgagca tcgcctgcgc
    28861 agtggtttcc gaaagttcgc tttcaaacgc gatcacggaa ccaaacgccg actgcctatc
    28921 accagagagc gcgcgcaagt aggaatcgac ttgggaatcc gcaaccgcca ttccgcatgc
    28981 ggctgcatgc ttgacaatcg tgcaggcagg acgaccaaac agcgtgcgca gaccgaacac
    29041 cgcgcgatac gccgcatcga catccagcag gttggtatag gaaagtgcct tcccttgcag
    29101 tggtgtcgca tgcgcggctc caatcggctc ggctggatca cggtataaga atgcttcctg
    29161 atgcggattt tctccgtagc gcagcgcctg cgttcgcaca aatggaccca gctgcaaagg
    29221 gaagcgcgat tcctgtgcgg catcatccag cgacggtcgt gcaaaccaat cggcgatctg
    29281 ctgatcgtac tttgcgatct gagcaaacgc ccgcgccgcc agtgcttgcc gaagctccgc
    29341 aggaagtgga ccttgttcga tcgctgcgat gatctgtgga tagtccgctg gatcgaccac
    29401 caccgtgatc ccacctgcgt gatttttggc agcggcacgg atcatcgctg gacccccaat
    29461 gtcgatcatc tcgatgatct gcgctgggct ctgtccgcga gcgactgttt cagcaaacgg
    29521 atacaggttg cagaccaaga gatcgatcgg aacgtatccg gacttttcca gcgcggccag
    29581 gtgttccggc tttcgtcggt cgaccagcag tccggcatgg atcttgggat gcagcgtctt
    29641 tacgcgcccc tctaggatct cttctgcgcc ggtaaattct tcgaccggaa tcaccgaaag
    29701 acccgctgac aaaagatgct gcgccgtccc cccagatgca agcagcgtca tcccttggga
    29761 aacaagcgca gatgccagcg gaacaagtcc acgcttgtca aacaccgaaa gaagcgcgta
    29821 gcgagccgca gatcgttggt ccatggaatc actccgtttc aaaacgtgtg ggggctgcaa
    29881 gtccccccgg tttgcggacg atgccttgat gaagaggtcg accgttttgc aagcgatcca
    29941 aaaagagctg caccgcgcgg ggatacagct cgtgttcgat ttccaaacgg atgcggtcgg
    30001 ccaggccatc gactccttca tcccctcgca ccagcagtga accggaaagc agaatcggtc
    30061 cggtgtcgat tccttcatcg acaaaatgga ccgttgcacc gctcactttg acttgtgctg
    30121 cccactgatc agccactgcg tgggcacccg gaaaatccgg aagcagcgac gggtgaatgt
    30181 tcaatatatg tccagcaaag ccgcgcacaa agtccggccc aagcacccgc atgtatccgg
    30241 ccagcagcag atgatcaacc cggtgcgcgg acagcaccgc agacagcgcg gactcatgcg
    30301 agctgcggct taccccagca tgcggaatgc aaaccgtggg aacgccacgc tccttggcgc
    30361 gatccagaat cggcgctgcg cggacattgg aaacgaccag caccagatcc gctgcgagca
    30421 ttcccaaacg aaacgcatcc agaagcgcga ccgcattcga tccgcgccct gatccaagaa
    30481 tcgccaagcg tggccggtta gctgcgggca taacccaagt ccacaccgcg ttcaccgcgg
    30541 acgacttttc cgacggggcg caagtctccg cagctcacct gggagagcgt ctctgcatcg
    30601 gaaggaccca ttaccaaaag aaagccgatt cccatgttga atgtcttggc catctcttcc
    30661 cacgacacac cacccagtcg cgcgagcagc gaaaagatcg ccggacgaac atacgagtct
    30721 ggatcgatcg aaagacgcag accgggcgga cagagcttgt gtgcccgcaa gagaagtccg
    30781 ccgccagtca catgggccgc ggctttgacg cgcacctgct tgtgcaaaga caaaacatcc
    30841 tgcacataca ggcgcgttgg aatcagcagc tctttggcca agctgtgggg caattcggaa
    30901 ggggtgtctt ggtaagagag accggcgtct gccacaatcc gtcgcgccag ggaaagacca
    30961 tttgcatgaa tgccgctcga tggaaacgca tacagaagat cgccatcgct tatgtcggct
    31021 ttgggaacgt gcggcgcatc gacgattccg actgcaaatc cggccagatc acagtcccct
    31081 tcgcgatacg catcagggag ttctgcggtt tcgccaccaa gcagcgcaca gcctgactgc
    31141 tgacacgccg cgatgattcc actcgcgatg tcggcaagtg gtacgctgtc cagtcgacct
    31201 gccgcgatgt aatcgagaaa aaacagcggc ctggcaccgc gtggtaaaag atcatttaca
    31261 ttcatcgcaa ccaggtcttg accaataccg gaataatcct ggcagtcgcg ggcgacgagc
    31321 agcttggttc cgaccccatc gcaagttgca gccagcagcg gatcggcaag accggaaagc
    31381 ggcggacgga caagccctgc gtaggcgtca ccactgtcga ccacggcatt cccataggtc
    31441 agctttgcat gcttgcgaac aaacgcaacg aagcgatcgg cggcgtccag atcgactccg
    31501 gcatcgcggt aggtcagcgc gggcggcttc atgttggata ctcccctgtc atacaaccca
    31561 agcaaaacgg ctgctgctgc gtggcacggc gcagacctgc ttgcgaaagg aaacgaacac
    31621 catcgacacc gatctgttgc cgcagcacgt caatgggatc ttgcggtggt gtcgtgcgct
    31681 gagcagactc ctggcagaca tgaaacagaa gctcatccgg acttgccatg tcgattccca
    31741 agtgacacgg cgaaacaatc ggtggtgagg cgagccgcag ccagacttct ttggcaccgc
    31801 gttcacgcag aagctcggac acaaagcgcg cggtgttgcc acgcaccaag gaatcatcaa
    31861 caagcacgat ccgttttcct gccaccgatt ccgccaccac agacagcttt gacaacagcg
    31921 cttggcgacg cgcatcttgg tttggcagga tgaagctgcg gcccgcaaag tgactgcgca
    31981 aaagcgccat ttcaaatggg acaccgctcg tttgcgcata gccagttgcg gcggccacac
    32041 cggaatccgg aatcggcacc accagatccg gcatcggcaa gtcgcgatct tgcagggcca
    32101 gctctcttcc catttcgatc cgggcgcgct gcgctccgat tccgaacacc acagagtcag
    32161 gacgggcaaa gtagatcagc tcgaacatac acggcgcagg cgctggactt ggtgcaagca
    32221 gctgtgtctt cacaataccc tgcggaccca cttcgaccag ctcgccgcgt ccgacttcgc
    32281 agacaaactg tgcgcccaaa gcgtcgagcg cacaggtttc cgacgcaatc acaaaaccag
    32341 acggactgcc cgccagcacc ccgatgacaa gcggacgcag accatgggga tcgcgcagtc
    32401 catagatccg gccatcgcag cacatcgcaa gcgaatagga acccaccaca gactcggttg
    32461 cgtctttcag ggcagcggca aagctcgacg cgcggctgcg tgcgaccagc gcgacaaaca
    32521 gctcgctgtc catggtggaa cagagcgtga caccggctgc gaccagcgct gcggtcagct
    32581 ctttggcgtt gcgcaagtga ccgttgtgcg caagcgaaag cggaccaaac ggggtgtcgg
    32641 cacagatcgg ctgatggttg tccggatgtt cttccgcaac ggtcgaatag cgaacatgtc
    32701 cgatggcgcg ggtcgcagac agagacgcga ccacagacgg ttttagcacc tggcgaacca
    32761 gacccgcacc gcgaatcgca tgcagcgatc caagctcagt cacgatggcc agacctgctg
    32821 cttcctgtcc gcgatgttgc agcgccacca gaccccgatg aacaaaacga gccgcgagcc
    32881 cctgcggatc gtacaccgcc atcacgccac agtattcgcg cggcttgtct gtttgggaag
    32941 acaagacggg aagcccggat cgcatcagca tgccaccgcc cgactctttc gcgcagattc
    33001 gatctcggtg agacgcagcg tgatgtccgc gccaatccgc agcacactcc cttgcgcggt
    33061 tcccaatcga tgaatgggaa tctcgtgctg tcgcgccagc aaaagcagcc gcgctgtcgc
    33121 atcatcatcc caggtactca ccagcgcttg ggcggggtgt tcaccaaaca gaagccagcg
    33181 cggcgcgtcg gtttctggaa gttccgcgac gattccgcac tgactgcgca gacacatctt
    33241 cgccagcgct gtcgccagac cgccagccgt caccgcgctt gcggcacaga tcagccgatc
    33301 agaaatcgcc gaacagacca agcgggccag ccgctgttcc aacgccagat ctacgctggg
    33361 aattccgcac ggacgaccca gcagaagccg cgcaagacga gaagcacact ggcgactcga
    33421 tagatgtccc agcacaaaca ccgcttgtcc gctgcgaaat tccgacacgg gaacgcgccc
    33481 gacatcagga atcacaccca gtccaccgat catcggggtg ggcggaatcg gtccgcgcgg
    33541 cgactcgttg taaagcgaaa cattcccacc cgtcaccgga acaccgagcg tgcgcagtcc
    33601 atcgccaaga cccctgatca cttcaacgat ctgtctgtgt tttttggcat ctcgtggtga
    33661 cgcgaaattc agaccatcgg tgatcgccaa aagttccgcg cccacacagc ccagccgacg
    33721 caccgattcg gccagggcgg cttgggcacc tgcatacgga tcggctgcac acagattccc
    33781 atcaccggtc aaggtcagcg caaaaccgcg ccgactattt ggcaaacgca tgacggcggc
    33841 ggcagcctgt ccgggtccgc gcacggtgcg attgccaacc gtttgatcga agcgcgagta
    33901 gatcggatcg gggtccgaaa gcgcagaaag cagatataag agacattcta tatgactctt
    33961 atcatcccct gccttcacat cgtcgatcgg cgctgtggcc agcggtgttt cgcaaagcgg
    34021 ccagtccgat ttgggtgcac cgccagcaac gagctgagcc ggcaaactta cgtgacaatg
    34081 gcccttgtga aagacgcgca caaggccgct gccggtgatt cgtccgatcg cagccgcttc
    34141 aagtccgtag ccctgaaagt gcgcaatcgc cgttgccaga tttttttcct ctatcacgag
    34201 cgcaaagcgt tcctgggttt cggacagcag gatttcaagc gggctcagat caggttcgcg
    34261 cagcggtacg tcgtcgagct gtatgtcaat tccgactccg cagggcgcgg ccatctcaaa
    34321 gcttgcgcag gcaattccac acgcccccag atcctgcacc gcgagcagtc cctgcgcagg
    34381 tccgaacgcc aagatcgctt ccaacagtcg cttgccggaa aacggatctc ctatctggat
    34441 gtgcgatcgc cgctgcgcaa agctgctcga ttcgcccagc gcttcgctcg caaacgcggc
    34501 acccagtacg ccatcccgtc cggtggctgc tccgacgagc aggagcacat ttccgactcc
    34561 ttgggcgcgc gcatgacgta gctcgtgcgg gcggacaagt cccaccgcca tcgcattgac
    34621 cagcggattc ccttcatagg ctggatcaaa gtgagtccga cccccgacaa tcggcacacc
    34681 gatcgcgttt ccatagccac caattccgct gaccacgccg cttgcgatcc gctttgtgtc
    34741 tgctgtatcc ggactgccaa agcacaaaaa atcgagcagc gcacagggtc gcgcgccttg
    34801 cgcaatcaca tcgcgcaaaa ttccaccgac tccggtggcc gcaccttgat agggctctac
    34861 cgcgctcgga tggttgtggc tttcgatctt gaacgcgacg gcccagcctt cccctaccga
    34921 taccacgcca gcgtgcgccc ctggtcccgc caagacgtgc ggactgtctt tgggaagtgt
    34981 cgacagaaga tgtttcgttg acttgtacga gcagtgctcg ctccacattc ccgaaaacac
    35041 cgccagctcg ctgtgggtcg gcgtccggtc cagccgcttg cgaatcacgg cgtattctga
    35101 ttccagaagg cccatccgtt cggccagtcg tcttgccgtt tcttcgtcga gcattccctc
    35161 aggtggaaac gctgcgtggt cctgcacatc gcttgcactg cgcgactttt tcatggtgtc
    35221 tgtacccgct gcaaaaatcg gaacgcactc tcaaacaagc gcagaccatc ttgcgatccc
    35281 agcagaagat cgatcgcgcg ctcgggatgc ggcatgattc ccaagatccg acctgtctcg
    35341 tcgctcacgg cggcggcatc aagctgcgat ccattggggt tgtgttcgta acacaaggga
    35401 atccgcggca ggacggaatg accatctttt ggcaaacggt acgcgccttc tgcatgcgcg
    35461 atcggcaaag tcagcttggt tcctacagga agcgtcgaaa accacggact ccgctccgcc
    35521 gtttccctga ccaccacagt cacaaagcga tgcacaaagc gcggcggcgt gttgtaggtc
    35581 aaagcaccgc gcagataacc cgcttccgcc aaaatctgga agccgttgca gatgccgatg
    35641 acaaggcctc cggacgcatg aaaggaagca agctgccgga cggcgcgcgc gcgcgaagcc
    35701 agcacaccgg cccgccagta gtcaccatag gaaaaaccgc ccggcagtcc gcagagcgcc
    35761 acatcaccag gaatgtcgtc ggtgtcacgt acatactggg tatcaaagcc agccgcacag
    35821 agcgcttcat atagatcgcg atcgccgttg ctgccaggaa atacgatcac cgctgctttg
    35881 atcggatttc gcacgctcac gatgcccccg ctccactatc tgttgcgcgt tctgccgcta
    35941 cagccggcgg atcagcaagc agccgcagcg agtacgtttc caagttggga ttcaccagaa
    36001 actgctgaca gactttgtcg gccagcaaga gcgcccgatc ggcatcggtt tcctgtacgg
    36061 aaaagcgcaa gtagcgaccc accacatcgg cacgtaccga cgaaaaatca ctgtgcgcaa
    36121 gtgccgaagt gaccgcttca gcctgcggat ctcgtgcccc gactacgggt gtgaccacaa
    36181 gttcaaatcg gaacacgatc agctctcctt cccaccgcta ttcggtgaca gacccagtga
    36241 acgaagcgcc ggtagttttt ccaaacgaca gagcacctcg cggtacgcag gcagaagctc
    36301 tgccagatcg ttgcgaaaca catctttgtc cagtacttct ccgcttgcga gatcgcgcaa
    36361 gcgacaggtg tccggggaaa tttcatccgc cagcagcagc gcagaatccg atcttcccat
    36421 ttcaaacttc agatcaagaa gcgcaagctg cgcctgcaga agcaaggaat gaagcttggc
    36481 cgccgctgac agcgccagct cccgaatcgt gcgaagctcg tcggtggttg ccagattcag
    36541 cagccgcaca tgatgttcat tgagcagcgg atcacccaag tcatcgcgct tataatacag
    36601 ctcatagaca ggtggttcgc agcgcgctcg atatggaagc ccggtacgct tttgcagcga
    36661 tccggccact tcaaaacgca ccaccacttc cagcggaatg atttcgaccc ttcgcaccgt
    36721 aagctggttg tcagcggttc gccgcagaaa gtgagtcggc gttcctgact gctgcaaata
    36781 ggccagcaaa agctcgctca tccgacagca cagaattcct tttcccagaa acaccgcatg
    36841 cttttttccg ttgcccgcgg tgacgtcatc tttgaactcg atctctgcca atccatcgtc
    36901 ggaaatccga actcgcttgg ccttcccttc gtataccgca gcgtgaacca acattccgac
    36961 accctgctgt ccacgccgca acgcgcagac agtcctttgg caatggaaac agctatcccc
    37021 gcacacagaa aacccgcgat tgcgagtcga tctgtgtttt gctgcgcggc ttcttacagg
    37081 cagaattttt ttcagtcaag taacaaaaac gaaacaacga tgtgacattg aattcccaaa
    37141 cggaattcct tgacgattca agttgcggag cataaaagcc cgcttgccat gcgcaacgtg
    37201 cactcgcgga tgatctccgc gctttctggc cctttggttg tggctgccgg tccgtggtcg
    37261 agcggagaat gcacagggga accactgtcc gctgccgatg cggtggttgc aaagactgtg
    37321 acgtccgctc cacgcagtgg gaatccgccc cccgtcctgc gaacactccc gcctgcgaac
    37381 ggccagacgc ctgcgggcta tctgaatcgc atcgggctgc gcaacgttgg cgcccaggca
    37441 ttcgttcgtg atcgcttgcc gcttgtgcaa aggtgcggac gtccgatcat tcaaagcttc
    37501 accgcacaaa cggaagccga ggtcgaagag atcctcgcgc agcttgagcc ggtttccttt
    37561 cttggttatg agctcaatgc atcgtgtccc aatgcgacat ccggactgct ttccacgcag
    37621 gcgctgcgca ccgtgcttgt gcgtgcacgc gcgctgacgc aaagacccat cacggtgaag
    37681 ctggcgtacc agagcgccga cgagctgctg gcgaaagcgc gcatctgtga agactgcggt
    37741 gccgatgcac tgaccgcgat caatacgatg ccagcgctcg atctcagtcc gattccgctt
    37801 gcttcggaat cgcagtttgt cggcggtcta tcaggtccgg gactaaatcc gtttgcgcag
    37861 tgggcggtgg ctcagcttgt gcgcgagcag tcccttcccg tcatcgcgtg cggtggaatt
    37921 cacagcctgc gccaggtgct cgcgttttgc gcgctggggg cccaagcggt gcagattgga
    37981 tcggttctga tcgaagatcc acagcgcgtc acaacacttc gcgctgaact tttcgcagcg
    38041 tacaaacaac atcaagtcga ttcctttgct gcactgggag agcgcttgtg tcagccacac
    38101 taaagctcac agaacgactt cactgccgag gaccactttg catcggtctg gatcccgatc
    38161 cgcgcttgat tccgtcgtgc gtgcacgggg atgtcggcgt gtttctgcgg gagatcatct
    38221 tggccaccgc agagtttgcg tgtgcgtaca aagtgaatgc ggcgtttttt gaagcgctgg
    38281 gaatttcggg aatgcatctg ctagagcgcg tccgcaagct gcttccgagc gattcctatc
    38341 tggtgtggga tgccaagcgg ggggacatca ccaacaccaa tcgtcactac gcaaagagcg
    38401 cgttttcggt atggaatgcc gacgctgtca cggtgtctcc gtacctgggc ttcgattccc
    38461 tttcgccctt ttttgattac agcaatcggc tgacgtttgt gctgtgcgcg acaagcgaag
    38521 gaaccgagct gcaagagctt gagacgttgc cagaccgcaa gccactgtgg ctgtgggttg
    38581 ccaagcaagc cgcaaaacga tcccgcggac agatcggtct ggtggtgggc gcgacccatc
    38641 cggatcgaat ccgcgccgtg cgtgaagtgg ccaaagacat cccgctcctg gtccctggtg
    38701 tgggcgcgca aggtggtgac attccgtcgc agccggcgct tgtcaatgtg tcgcgatcga
    38761 tcctgtatgc cagcgccgat gctgactttg ccgcctgtgc acatgccgcc gcaaagtcgt
    38821 ttcatcagcg gctgatttct gcagcaaagg aacccccgca ccatgatcac tccgcccact
    38881 tctgaaacgc agccttcgct tcccatgctt cttggcaaaa cggaagcggt gctgcacggt
    38941 cacttccggc tgagctctgg acttcattcc gatacgtaca ttcagtgtgc gcggctgctt
    39001 tcgcatcctg cgtatgccca gcaaatgggt cacgacatcg cgcgaaaagt ccagaaaagc
    39061 ggcgtcggcc ccatcgacgc tgtgataggt cccgcgctgg gcggaatcat cattgcccat
    39121 gaagttgcgc gtgcgctgtc tgtctgttcg ctgtttgcgg aaagacaggg gagcgatctg
    39181 gcgctgcggc gtggcttcag catcaaaaaa ggccagcgga ttttggtggt cgaagatgtg
    39241 atcacaacag gcaagtcagc aaaggaaacc gcatcgctgg tcttggcgct gggtggcgtg
    39301 gtggtgggtt atgcttgcat cgtcgcgcgt ggcgattccc acgcgctttc tccgcttcat
    39361 tgcctctgtc agctgcgtcc acaggttttt tctgaaacag agtgtgtcct gtgtcaggaa
    39421 ggagtccctc tccacaaacc tggaagtcgc ccagaagcgg tgtgatcacg cagctacgtg
    39481 ggcggtggcg gtggattttt catcggctcc ggaagctcac catgctcggt atgtggcggc
    39541 tgtgcctgtt gtccggacgt accttgcgat ccggcctgct cggacttgat gtgcgcggcc
    39601 agattttcga tgctgcggat cacctgttca aactgcggac ccgagatcat aaaggccgga
    39661 acggaaccac cgggctcgct gtacgtttgg tatgtgacat gggaaaatcc atcttttccc
    39721 ggttcaggct cgacgagcca gcgaccgctg atcgccggaa tccgaacata gtgcggatca
    39781 agcggcggac cttcgtcatt ggcagtctgg aacgtcattt cgtaacgatc atttcccagc
    39841 gcttgggtac gcagccgcat cgtgtagtca cgatccgaaa caaccggcgt cttgatttga
    39901 tcgtaaataa gaagcgagtc cttgctcttt tgtagcacct gccgcttcct gacaaccgga
    39961 cccgtgcgcg tcggcaccgt gctccacatg agctcgatga gcgccgctgg tgccatcggc
    40021 atcgtagaga cggcgcgata ttcgtaaaag gaagaccctt tcactgcgcg acgctcaacc
    40081 gcgactccgt gcttttcctt gtacttttcc cacggcaccg tcgacttggc gaccacatcc
    40141 aagatcgaat cggcatacac aagtcgcgga acgacaagcg acaacatccc cgcgaaaagc
    40201 cacccacttc cacccatacg agagcaagtt ctattgcgca cagtatttcg catcttgcca
    40261 gaaccccaca agaagcgaaa gccagcgggc cggaatctta gcggtcgtcg tcggtcattc
    40321 cgcgctcttt gcgcgcacgc aggcagtcgc catcaccggg ctgcacggtg cgacaggctt
    40381 tgggacggtg gtgatacagc tcgcacgaca cctttttgaa cagcacaccg cgcagcgcca
    40441 gacaccgacc atcggaagcc agccgcagat gcggatgccc catgtcatcc atcaccacat
    40501 acttgcgaac gaggtcgcga cgcaaaagca gttttgccga tggctcgacg gacacgtagt
    40561 ctgttcgacc ttccgcttcg ttttctttgg cgtttacaca gcacgcacca cagcgctgac
    40621 agtccagttc cacgcgcgcc gttttagcaa agccagaagg cgtttccatc ccccatttct
    40681 gcgtctactt tttggctggg tccggagtca ggatgttttc gactcccagc tcgccgagaa
    40741 atggacgcag cacatcaatc accgcacgaa tgcgtgggat ttcccacagg acagccggaa
    40801 tcatcacgcg catcccgaca tcttttccga tgagatcggg cagcaccgga accagcgttc
    40861 catccggcac cccgggatca ggaagcatcg cgtccggaac cagcgcaatg cccattcccg
    40921 cgatggcaaa ctgccgaatc aagtgaatgt ggcgcgtcac cgtcatcgcc gataccggat
    40981 aggagccccc acgcagaagt ggccacacct tccgatcatc ccccggaccc acccaggtca
    41041 tcagatcgtg gtgttttaag tcatcaagcg tgcgtggtgt tccgcgctgc tgtagataat
    41101 cccgacttgc cacggcccag acgcgcgaac ggacgatctc tttggacacc cacgccccgg
    41161 acggacttgc gtccccaaag tgaaacgcca gatcgacatc ttcgataagt cctccggtgg
    41221 gatcgtcact aaactgaaga tgaaaagaca gccgcgggta cttgtgatgg acaaacccga
    41281 gcatcggtgt cagcagatgc ggcggcagtc caattggaag caagacccgc aacatccccg
    41341 atggctcgct tccaacttcg cgaaccgacg cgatgagcgc gctggcttcc tgcaccatca
    41401 gccgtccccg cgccgccaag acccgcccgg cttcggtaag catcacaccc acacgggtcc
    41461 gatccaccag aaggactcct gcccgcgctt ccagctgatc aatccgccga cgcagtgtgg
    41521 cccgcgaaag acgcagtgac ttggccgcgg tcaaaaaaga accgctgtcc gcaaccgcca
    41581 gaaaggcgcg aagttcttcg agatccatgc ctgataagag gcgatggcca cttctgtgtc
    41641 aaggcatggc cacctgcgga cattcctatg gcaccgcgat gcacctacca ttttgacatg
    41701 gcggacgaaa cacggaatcc agatggtttt ggcaagctct tgtggcgcgg cgagtccgga
    41761 tatgaaacgg cgcgtcgccg atcgatgtgg aatcagcgca ttcccgatcg ctttcccgat
    41821 gcgatctatc gcgtccacga tgtctacgat gttgtgtccg cagtcaagct tgccaagcag
    41881 cgcgggctga acgtatcagt aaaatcgggc gggcatagct ggtcaggaaa tcacctgcgc
    41941 gaaggaggac tccttatcga catgtctgcg atgcaggctg cgacgatcga caaagccgcg
    42001 atgcgtgcaa ccaccgaacc cggaaaaggt ggatcgattc tggccgagat gctactccgc
    42061 caaggactgt ttttcccgac cggacactgt cgcggagtct gcgtaggggg attccttttg
    42121 caagggggct ttggctggca cagccgcacg ctcggacccg cttgcatgag cgtgattgga
    42181 cttgatcttg tcaccatgga cggcgatgtg gtgcacgcaa gcccgacgga aaatgccgat
    42241 ctgtactggg ccgcgcgcgg gtccgggccg ggcttttttg cggtcgtgac gcgcttccat
    42301 ctgcgggtct atccgcgtcc gcgggtgatg ggagtcgcgt atcaacatca cccgatggaa
    42361 cagctagaag atgtgtatcg gttcgcccat cgcgtcgcgc ccgatgtccc aaactcgatt
    42421 gagctgcagt ttatcatgag ccgacgcgca tcgtttttgc caaagcccgg tatcgaaatc
    42481 ctggcgccca tttttgccga cagcgtcaag gaagcctggc gggctacgga gtttctgcgc
    42541 gatgcagaga tcggtcggca cgccaccctg cgggttccgt ttctgcccgc caccctgagc
    42601 atgatgtaca ccgcggcgat gcagcactat cccgatgatc accgctgggc cgtcgacaac
    42661 atgtggacgc gcgcgccgat tgatgatctg ctgccaggaa ttcggcggat tgccgaaacc
    42721 atgccggaag agccagcgca ctttctgtgg atgaactggt cgcccccaag caatcgtccg
    42781 gacatggcat tttccgtcga agacaagacc tatcttgcgc tgtacggtgg ctggaaacac
    42841 gccgccgatg acgaaaagaa cattccgtgg gccaccgata ggatgcgtga aatggcgcac
    42901 ttgtcgacgg gatgtcagct cgccgatgaa aacctgcgga ttcgtcccag tcgttttctg
    42961 accgaagcca acatgcaaag gctcgacaaa atccgccacg atcgcgactg cgatctgcgc
    43021 tttcactcgt atcagggaag accatgaacc atccccatcc caaacgaatc ctccagttag
    43081 gaatcagcca agcggaacag cggcaaaaac cctacggtca cttttacaat cccaagcttg
    43141 gcgcgctgcc ttcccatgcc aaagaggcac tgctgatagg gccggtggcg gggccggttt
    43201 tgccgacgct ggctgatgcc ccactgcttc tgacttgcga agaaacagag ctggaaaacg
    43261 gtctgggaat cagcgcagat ggctgtctgc atgttgcgat acgcaccgat cttccggatg
    43321 tcacgcccgc gatgatcgac tggtggtttg gttggcacag cgcagaaccg cagcgctaca
    43381 agctgtggca cccgcgcgca cacgtttatg cagcctggca agatgtggaa acgcaagagc
    43441 tggccgcgca aaaaggacgc ccgcgctatg tcggtcgggt ctcctttgtc gacgagtatc
    43501 tgggcagtac gctgacgcac gcagcgatcg gttttgtgcc gccaaagacg ctcggttttg
    43561 atgaagcgct gctcgcagat gcgcagaaac acaccgtgat cgcggcgcgg gtcagtctgg
    43621 ccgaaacccc gctggatgcc gggtatctgg ttcaccatgt tcgcaaggtg ccaggcggtt
    43681 cggaaatgcg atcgcgtttc tggctgggtg gtccgtatgc cgcagtgcaa gccggaaacc
    43741 ggcgcgttca gattcccagt gcagtgtcgc gccggattcg cgccccgacg ctggatgaag
    43801 gccgcgacct gctcgtgcac tgctcgcaag agatgtcaca cctggcgacc tttcttccca
    43861 aactctatgc ggagtttttc gatgtagcat agaaaggtca aagtcccagg cacgctgggc
    43921 gacatcgtga tggtctgact gtccctgcta tggtctgtcg gtatgccatg aaccctaaag
    43981 gggtcccgcg gtggaggaat ttgttttgga gtttgcgcgc gctgagcaag cgggcgatcc
    44041 atttgctttc cggtttaccc cccagccata tctgctgcgc acaaagggcg gcggctttca
    44101 gtctgccgag ttcccgtgga gcgaagcgct gcttgacgat ctggaatcgc tgcgcaagcc
    44161 gcgacccgat cgagcgatcg tgcagcgcat cggtgaactt ctgcgtcggt ttgtgacacc
    44221 gctcggcgtt gaagcgcagc tcgatcagat cgtgactgca agtcgcgccg gcaaagaggt
    44281 ctgcatcacc ttccgttccg cggcagcaga gctatatgcc ctgccctggg agcttttggc
    44341 cgaccggcac agtggtcaag cgatcggcag tctgcccggt gttctgtttc gttatgagtg
    44401 gccggaatcg aagaccctgc aaagtacagt ccgcagtgaa ggaggccgga ttctgtttgc
    44461 atggtcggcg gcgcagggcg cagtgccggc caaagaacag atcgcggcca ttcgatctgc
    44521 gtgcgaaaag gaatcgctgc cctttgattc cgaaagcgat gtcatcgccc acgcaagctg
    44581 tgacaagatc gtttctgcac tggaaaaagc ccgcgccaca agtcgtccgg tggcgatatt
    44641 gcacattctt tgtcacggtg gcgaacaggg cacaacgttt ggactgagtc taaacggcga
    44701 agaagaagga gagcccgaac acgtcgacgg tgcgaccttg cgacagctcc tttccccgtt
    44761 tgccgacatg gtgcagcttg tcgtgctttc ggcctgcgat tctggcaatg ggggtgcgct
    44821 tggcaatcag cttggcagcg tggcacaagc gcttcatcgc gccggttttg ccgccgtgct
    44881 cgcatcgcga ttcccactgg caattcgcgc cgccaatcag cttgcagaga cgctgtatga
    44941 gggtcttgtc tgcaactctc tttccgtcga aaaagcgctg ctttcggtgc ggaatcgact
    45001 tgtgcgcgat ccggctttgt ttgactgggc cagcatacag ctttacgcgc gaacgggtga
    45061 tcaaggggac ctgcgtccgc tcctgtttcg tccttaccgc ggactgctcg cattccagcc
    45121 agaacatcgt gcctttttct ttggtcgcga gcgcgagatt gatcaggttc tgaccgctct
    45181 ttcatcgctg cgcagcgctg gaaaaccaaa gtttgtgatc gtcgctgggg catcgggaag
    45241 cggcaagtct tcgctggtct tggctggcgt gattcccaag ctgtgtgggc agggaccgga
    45301 tcgattcctt tccgcttctg tgaagccggg tgccagtccg cttgaagtgc tcaaacagat
    45361 tccggaacgc acagacagac cacttttgct ggtgatcgat cagctagagg agctgtttac
    45421 ccacgttcca gatccggcgg tgcgaaccca gtttgcgcaa aagctgtggc ttcttgcgca
    45481 gagacccgat gtccacatcg tggcgacgct gcgggtcgac ttcattggtg aatgtggcga
    45541 gcttgtgatc gatgaaagcg gtcttcggct cgatcgaatt gcctacgatg atgcgcaccg
    45601 ggtgtttgtt gcgcagctcg gtcccaaaga gcttttggcg acgattgagc agcctgccgc
    45661 gcgggtggga attgcgctgg aagccggact gaccagtcgc atgctcgaag ccgtttcggg
    45721 tgaaccgggg gcgcttccgc ttctgcaaca tacgctggat cagctgtggc tgcgacgaga
    45781 acacagaacg ctgacccaga aaggatatga tgcgctcggc cagctaggcg gtgcgctgtc
    45841 ccaacatgca gagtcgctgt ttggcaagct ctcacaggag gagcagcgct cggcgcaaga
    45901 ggtcatggtc cgcttggtgc gactcgaaga cggaatgaca cgcgcgacac gacagagagt
    45961 cctcctagac aagctccgtc cgcgcaaaaa ggaatcgcag gcgcgcttcg atggtgtgct
    46021 tcgcgacatg acggcggcgc ggcttttgac catcgctggg gaagatccca attcgacggt
    46081 ggaagtcgcg catgaagcgc tgattcggag ctggccgcga ctgcaaaact ggctcaagga
    46141 agatcagact ttccttatcg agcttggcaa actggaaggt cttctgcgcg agtggaaaga
    46201 acaccacgca ctgcttgtat ctgatcagct ccgctttgcg gaacgacttc tgcgaaccta
    46261 tccggaacga cttccggaag agacggcgca gctcgttcgc gaaagccaaa gggaggaacg
    46321 gaaaatccgt ttgatccgac tcactgcgct gctggttttg gtctgtggtt tgatcgtgca
    46381 aagctggatg tttttttcgg aacaaagagc gcacaagcgg gcggagctgg cgcgtcacga
    46441 gctgatcaat ttgatgagca agaccagcca gttaccgtcg cgggcagagc ttttgcggac
    46501 gctcgcacgg ctgcgacttg gactggagct aagacccgat ctggcgcttg tggcgatcgc
    46561 agaagcccag aaactggaac cggaaaatcc gcggctgctg gtcgatcaag cagagtattt
    46621 tttggcggcg gcgcgctttg atgaagtctt tttgccggcc cagatcgcgt ttgaaaagga
    46681 agccgatccg gcgcgcaaag tgttaagcgc tgcaatcgcc tggagcgcgg cgcgacttca
    46741 tcacagaccg atcgaacaaa aggcgtggag cgagcgacta cagaacacgt tccacagctt
    46801 gcccgaaggg gtcgaagtcc cagagcttta cgcaacgtcg cagcttctta tcgaaaatca
    46861 gtcgctgctt cggcaggctc tgccgattcc tgatgtgctg gcggtgttca agctgatcca
    46921 cagtcccaga acaccgcagc gtgaagacga tctggccaag cggctgcgcg tcggtctgta
    46981 gttccgcggc atcatcgtgt ctgtgacaaa acgacgcgcc gttacagagc cgcgcatttc
    47041 ggcgcgccca aggacgcgat tttgtgtatc ttgcgcgacc gtgaaactgc tgctgacaaa
    47101 tgatgatggt gtgcgtgccg acgggatcaa tgtccttgcg agcacgctgt ccgatgttgc
    47161 ggaagtgttt gtggtggccc ccgatcgcga gcggagcgca acgtcacatt cctttacgct
    47221 gtttgatccg ctgcgtgttg aaaaggtcgg acacaatgtc tggtcggtgt ccggaacgcc
    47281 tgcggactgc gcgtatcttg gcgtgctcga gttttgcaaa ggagccgact tggtgatttc
    47341 cggaatcaac cacggctaca acttaggagc cgacattttc tattccggaa ccgtcgctgc
    47401 ggcggtggaa tcagcgctgc gtggatactc tgcgatcgcg atgtcgatgg gaatgggtcg
    47461 gggtgcagac ttccatcgcg ctgcgcggtt ttgcagagct ctcatccaag gaatgcaagg
    47521 gcgtcgcttg ccgccgcgca cgcttctgag cgtgaatgtt ccagggccca aggaaccgcc
    47581 tcctccgcag gacggacccg cgtatcagat cacgcggctt ggcgaacgga tgtaccgcga
    47641 tcacgtcgac agccgcaccg acccgcgtgg caacacctat tactggatcg gcggacctgt
    47701 cgcaacggac agcgagccgc cgggcaccga tgtgcatgcc attcatcagg gactgatttc
    47761 ggtgacgccg ctgggactgg accttacgca tcggcagctg ctcggcgatc tgccgtcgtg
    47821 ggatgtcccc ggctatcgcc agatcgggac gtgatacgct gtgtgggcaa ggatttttcg
    47881 atgaagccgt ttacgacgct gtcccaggag ctgctcgcgg actatcggat ttttcgtctg
    47941 cgcaaagatc gctgccgatc gccgcgcacc cagggagagc atgactttta cgtgctcgaa
    48001 gcggattcat gggtcaacgt ggtggcgctg acaccggacg agcagatcat tcttatcgaa
    48061 cagtatcgtt tcggtgtcgg gcagatcacg ctggagatgc cgggcggaat catcgatcca
    48121 ggtgaaacgc cactggaagc cgccgcgcgc gagctcatcg aagagaccgg ctatcaggcc
    48181 gatgcgatct cggtgctcgg gcatgtcgat cccaatccgg cgatcctcaa caaccgctgc
    48241 tataccgcgc tggcgctcgg ctgccggaac atcgcgggtc agtcgctcga tgaaaaggaa
    48301 gacatcgcgg tgcggctgct gccgattgcc gatgtgccgc agctgctggc gtccggacag
    48361 atcacccacg cgctggtctg ggccgcgtac cttcactacg atctgtggaa aaaaaagcag
    48421 ggctcggcgc gctaagcgcg ttcacagccc gagcggattg acccactttt tgcctttgat
    48481 cggcaccggc ttgggcttct tttccggcac aggtggcggc agcgcagccc gacgcgcttt
    48541 ttccttttcg atgccgtttt tcacttcctg ccacgcctgt tcgtaaagcg ccgtcgcatc
    48601 gccgacatcg cgcagtggtt cgtaacttcc cgagtgactg cgcagcgatt ccagtgtccc
    48661 ccacagccag cgcgcttcgg tgtcgagcat cttccgcccg ccctcgctca ccgtcggcaa
    48721 aagccgcgtg ttggcgatgt ccgcggaaat cgccggttcc agcaggtggt ccaaaaacgc
    48781 aaacccaaca tccggctgcc gtgcggactg gagtacgcag gcaaaatcga catacagcag
    48841 cgtgccttct ttcggcagca gaaaccgcac ctgcggcact ttgcgaacca gcgaaaacac
    48901 gtcgttggac cagcccatgc tcacagacag cttgccgcgc ttgtccagat ccgccggatc
    48961 gtcgatcagc tcgatgccgc gcggcagcgc ccgcaaaagc agctctttgg ccgctgcaat
    49021 gtcggcagga tcgcgggtgt tggccgactt gccgagccgc cgcagcgcca tccccagtgt
    49081 cgcccgcatg tcatcaagcc acgcgacctc gggaccgctt tcggcaaaca ggatgtccag
    49141 gctcgacggc tcgatgacgt gtccggtgcg cggcggcaga taggcatagc caatcaccca
    49201 ccagacatac ggcacgcagt gcagcaggct tgggtcatac ggcggattgc gaaaatcgag
    49261 cggcacctgc gccaagttcg gcacccgctc acgcggcatc ttggccaccc ggcccgtcgt
    49321 gatcaggtgc tcgacggcat agcccgaaag aaacaccaca tcaaagcggc ggctgtcgag
    49381 gttggcgagc agcgactcgt tcgactgaaa gacctctagc tcgatgtgat tgccggtctt
    49441 cgcttcaaac gacggcaggg ttcgcgatcc gaaatagtcc gcaaacgtcg ccacgcgcag
    49501 cgtctgtccc gacggaatca caggcggtgg cggcacaggc ggcggcggac gattacacga
    49561 cgcaaatagc ggcaggagca agctcaccat gggaagctca cgccgcagca agttgcggcc
    49621 aaaagagcga agtgcctgcg cagccttctc cagcatcgaa gctacctcga ggatgcacag
    49681 agtgcggtcg gaccggcaag tgctttttcg cacctttgga cccagcgccc gtcgatcgag
    49741 cgaagaccgc cgagcgacga ccgcgttgcc ggactacaga tcgcgcacgt cccaggtcat
    49801 cccagtccgg cgcagccgat cgggaagatg actgcccatg acaaacgccg gcgtcagcac
    49861 accaccctgc gtcggcagcg gatcttcggc cagacacagc gccgcttccg acagcattcg
    49921 agatgtctcg ccgtaccccg gatcgtgatt gcctttgacg atgcactcgg cgcgtcgcac
    49981 ttcccccgac gggagcggct ggcttttgcc gatcagccga atcacgaaaa agccgcgatt
    50041 gcgcgcccgc tcgctcgggc cttcgcctgg tgccggcagc cgtttttcag ccaagccacg
    50101 cagcgtgtca ctcgatccaa ccaccagcat cgccgctgtc cccgccgccg tcgccaccga
    50161 tcgcagataa ccggctgggg ttttcggatt ggcgatgact tcggcgtagc gaaagtcgcg
    50221 accataggca aagttcgaca gcgcattggt ccgtcgcacg atgcgggtgt tgatcgccgc
    50281 catcacaaac ggcgccgtgt agtagcccgc ctcgtcgtcg gattcgacgc ctttttgatc
    50341 gccccgatcg gggccgcggt ctttttggcg gtccggatac agcgcgtagg gatcgcccat
    50401 cagacgacgc agctctttgt cggcagcggc ctcggcaatt aggttcagtc ccgacgcgat
    50461 ggtgccaccg ctcgccccgc cgcgcagcgc caccacgtag tatttggccg accccagctt
    50521 gagacctgat ttggcaaagt gctcggcgag caggtgcacc cccaagtccg acggcagcga
    50581 gtcaaaacca cagcagtgga cgatccgcgc gccggtctgt tccgccgtcg catgcgcgct
    50641 gtcgatggac cgacggacaa actgcacctc gccggtcaag tcgcagtaat cggtgcccgc
    50701 ctgcgcacag acttcgacga gcggcaggcc atatttggcg taaggaccga cggtggtgca
    50761 gatgacgcgc gtctggtcgg cgatcttgcg cagggcgtct ttgtcggtgc tgtcggcaat
    50821 caggatcggc agctcggcca ggtttgggtc gatccgcacc agatcgcgac ggacttcttc
    50881 gagctttgtg cggttgcggc ccgcgatcgc aaagcgaaga tcccgacgct ggagacccgc
    50941 cagatactcc gcgacgagct tgccggtaaa acccgaggcg ccaaacagca ccaccgcata
    51001 cggacgatcc ccacgcgggg tcacagaatt agacatgcgc tgatcctagc gcgtcgccca
    51061 ggtcggcaag cactcgctcg acatggcctt tgactgacac tttgccgtag acctgcgcga
    51121 tccgtccgct ttcgtcgatg acgaaggtcg aacgaatcat tcccagggtt tttttgccgt
    51181 acagcacctt ttcgccatac gccccatacc gcttggccac ttccgcgccg gcatcggaca
    51241 gaagcggaaa ggggagcgcg tatttttggg aaaacttctg gtgcgaggcg accgaatcgg
    51301 ggctcacgcc aagcacgagc gtttttgaaa aacgcgcgct gtgatcgcga aaatcgcacg
    51361 cctgctgggt gcagcccggc gtgttgtccc tcggatagaa gtacagcacg atgcgccggc
    51421 ccaaaagctc gcgcagcgaa taggtcttgc cgtcgtgcgc cggcagggaa aagtccggag
    51481 ccatgtctcc gactgaaagc gtgggctggg tcgccatgtg gtttcggtgt cctttgaaaa
    51541 aattcggttt acgccgcctg acagtcgacg agcctgagca gcttggcccc cgagcgcaga
    51601 aactcgcgtt catgacaggt cagcaaaatg atttgatgcc ggctggcacc gcgccgcaaa
    51661 agccgcagca tccgatcaaa ccgcgcgtcg tcacaaaacg tcagcgcatc atcgagcaaa
    51721 agcggcaccg actgcccccg ctgcgccatc aggtccgcca ccgccagccg caccacgatc
    51781 gcaagctgct cgcgcgtccc caggctcagc gccacaaacg gctccagctg ctcgcctcgc
    51841 cacagtcctt cgatcgccag cgtcgattcg tttatcgcca gacggcagcc cggcaaaatc
    51901 gcctgcagat acggctcgca cagacgcaga agcggcccaa gatacgcctc gcgtgagcgc
    51961 tgatcgtgcg cctgcatgct ctcgtacagc agccgaatcg cttcggcccg ccgcgtcttg
    52021 tgcgatagct cgacctgcgc ccgctcatac gccgcctgcg tttcggccag ctcttcgtcg
    52081 atggcctgct gcccgagcgt ccgcagctcg atctgcagct cttgggcttt ttgcgtaagc
    52141 cccagcaaat gccgcgacag ctcgtcaatt gcggccttgt tttggctcac attctgacga
    52201 attccgtcaa gatccgccgc ttttagctca ttttcgagca tttccgcctg cgcacgcttg
    52261 tctagcacga cttgcttttg cgcttgcacg ctcacgtcta gctcggcgtc actgcactgc
    52321 gccgcctcgt catcgagctg ttttttgagc cgctcgactt cgcgacgctg ctgcgaaagc
    52381 cgaccatcgt gctcggcttg ctgattgcgc aatttttcga cgagcagccg cttgcgctcg
    52441 acctcttcat cgcagagcac aagctctcgc gaaagcgact cgacaagctg ctgccgctcg
    52501 cccagctgcc gaagcagcgt ctcttgcggc agcgacggct ctgtttcatc accgagctgc
    52561 acaagctgcc gcgtaagccg cacaatttct tcgcgcagtg ccgccacgcc cgacggacac
    52621 agccgcccca ggtgttgcat aagctcgcgc tgctcgtcga caaccgctcg ccgattcgcc
    52681 gcttcttgct ctgccgtcag cagatccgcg acccccagct cggcaagctg agccaagagc
    52741 ttcgcctgct ccgtctgcaa gctctcacgc agccgcgcag cccccgtccg acccggcgta
    52801 atcaccagct tgacgcccgg cagcacaagc tcggttcgat cggtcacaat ccgcgtcgtt
    52861 gctggttcag aaactggctg tccatccagc cacagctccg cagtccccgt gaccgcgacc
    52921 tccagcagcg tcgccacggc atgcagctcg acctcacgct cgcggaccag tcggtgcagc
    52981 tctcgcagct tggcgagctg tttgtcgtcg accacaagcc gcacaagctg atcggcgagc
    53041 tcattttttc gtttttccag cgtctcgact tcgccaagcc ggctgtttcg ttcaaaaagt
    53101 tctgccgaaa gccgttgccg gtcgacggag cgccgcagca gagacacctc cgccgatgcc
    53161 gccgaatgct cgtccgattt tttcttgcgc cgcgtcaccg catcgttcca cagctgctcc
    53221 tccgtcgaaa tcccgacgag cccctgttgc ttgcgcacat ccagcttcag cagttcttcg
    53281 tctgccagcc gcacggtctc gcggccatcg agtcgtcgct tttgtttttg ccgcagcagc
    53341 tccaaccgtt cctcggagag cgtcacatct ttttgcagcg cagcgagcgc acgctctcgc
    53401 tcggacaaaa gcagcagcgc cgcttcgtct ttttgcagcg ccgacttctg cttcgccagc
    53461 gttccgtctt cgtcgtagcg tcgcagcttg tcgcgcagat ccccgagccg atcgaccttg
    53521 ccttcgtagt cccgctgtcg ctcacgcaga ccggcgagct tgaccgccag ctgttcaact
    53581 ttttcccgcg cttttttcag ctcgtcgcgc ggctggccgg tcttggtgta gttctcgtcg
    53641 tgccgatcgc gaaacacctg gagcagtttg cgccccagct cacccgtcag cacgctgccc
    53701 acttcggatt ccagcgccga ctgcaaatca cgctggagcg acgatgcggc ttggtggttg
    53761 cgaaacgact cgccctgcgt cacccacaga agtccgcaca gaccttggtg ttcgcttttc
    53821 ggttcggcgc tgttggcttt cgccgggcta tcaaagcgca aaagccgcgc cagctcttcc
    53881 tcggcggcca tgccttcgag ctttcccgtc ggtgtcacca gttccgcgcg cggattttga
    53941 caaaacgctt tgtacagcga atacggcctg ccaaaaaact ccagatccag ccgtagctcg
    54001 ggcctgacct tgctgccaaa cggcaaaaaa tcctcggtca ccgtcgcttt gtatttcaga
    54061 aacagcgccg ccttcagcgc cagaagcagc gtcgatttgc cgtcttcgtt gtcgccggca
    54121 atcaccgtca gaccgtcgcc caggttgtcg atgcagatcg gcccgcgcag cttgcggaaa
    54181 ttgcgtacct cgattcgccg gagccgcatc gcttacgtcc ccaccgcttg ccggtattct
    54241 tcgacgagca gcttgagcgc cagcttcgcc cgctcgccgg cttcgctcgg ctgttgttgc
    54301 ttttgccgca gctcagcgac gacgtttcgc aaaagacctt ctgccggcaa gttgtctagc
    54361 tcatcttccg agacttcggg caaaagcgca tcgtcgtgaa cccgcaaaag atgaagcctg
    54421 gcccgccact cggaaagcag cttgcgcagc gactgctcgg cggaaagcgg cagcgacccc
    54481 atcagcgtca agtcgagcag cgtctgacgg tgcggctggg ccagtcccat gagcatcgct
    54541 tcgacgagcc cgacatcggc ttccgacaag acggtcagct tgcgcttttc ccactgaaag
    54601 tgcccagtct tcaccacgtc gacctgcggc ggacagccgg ggccttccat ctcgacgaag
    54661 agcacttggc cgcttggact gcgatcggcg tctttgaatc ggtcaggctc gggcgtcccg
    54721 gcgtaatacg tgcggtcggc aatcggcaaa aaaccgtgcc agtcgccgag cgccagatag
    54781 tcgagccgtg cccgctgcac ccgatcgtcg gcaatttcgt tggtggcttc gctgcgctcg
    54841 ggcagtcgat ttttcaccga tccgtgggcg acgccgaccc gcaccgcgcc aactggggaa
    54901 accatcgtgt cgagcgcatc gctcacatcg atcgattcgt gccgacgcag aagcggggct
    54961 ggcaaaaccg ccagcttgcc gtctttcaaa atcaccggct ctgccgaaag aagcggcttc
    55021 acattcgcgg gcaggcccag tcgcacaagc cgcgtccata cgctttcaaa aagcgctgcg
    55081 tcgtgatttc ccggcaaaag cagccaaggc cccgaatagc ccgccatcgc gaggaccgtg
    55141 cgccgcagca catcgtcggg caccgtatgg ctgtcgaaga catcccccgc agcgatcacg
    55201 cagtcggcct gtctctcacg ggccagagcg gcgatccgct cgaccgcaga aatccgctcg
    55261 cgcttgagca gcgcagcttg atcgggatct tctccgacga gatccgcgaa cactttgccg
    55321 atctgccagt ccgccgagtg aacaaaccgc accgccatcg caagctcccc acaagtcgtt
    55381 ttgggaacaa tagatcacgg cgggcgcggt gcaagccgac tctactcgcg atgataggga
    55441 ctgccgtcga gaatcgactg cgcgcgatag atttgttcca gcaccaaaag ccgcgccaag
    55501 cgatgcggca gcgtcaactt ggacagcccg agcagatagc ttgcccgatc gcgcagcacc
    55561 gcgctgtgac cgtcggcacc accgatgcaa agagccaaac ccggtgcgcc cgagtgaaat
    55621 gcccgatcga gcctctgcga cagctcccgc gacgatggct gctcgcctcg ctcatcgaga
    55681 acccagatca aatatcgcgg cggcagtttt tttagaagct gctcttcgtc tttgacctcg
    55741 atcacttcga cgggcaggcg cgggcgcaga cgcttttcat attcggcaca gccagcgagc
    55801 acccacggat cgcgcagctt accgaccgcc aagacgacca gcttcaatcg agcctacttt
    55861 gccgtcggtg cggcgatcgg ataagagagc gtctgtttca gccagtcggc atttttgcgc
    55921 accgcagaaa gcagctgctc gtagacggcg ggtcgcttgt caaacagtcg atatccgtcg
    55981 cggctgcgca gatagccacg cgctttggga tgcttttcaa acagcgccgc ttcgcccatt
    56041 ttttcggccc agcgcgcgta gcgagcccgg ttgtcgagca gcgccttgac gacaaacggc
    56101 atcaccatcg agtaatccgc ctgcatgctc tcggtggtgc gctgatacac gtccttgtcg
    56161 accttgcccc acgtcaccgc ttccgccggt ggacacgacg acagcgaacc gtctgtcacc
    56221 ggcgcgctca cgatctgtac gtcgaacgta tagccgcgca cttccgacag accaagcacc
    56281 tgcgaaagcc ccggctctgg ttgcaggttg tagtttttgg gcacgccgcc gcccaagatc
    56341 agcgtcgcca gcgcgcccgg ctcgacttgg aacagacagt gatagtggta cgcgcacgac
    56401 tcgaagactt cagcctgcac gtcgagatcg aagccaaact cggcaatcag cccggcctgc
    56461 ttcatcgccc acagcttcat cgcgttcaaa aacacgctgc catcgcccgg tgcaccgacg
    56521 aggatcggaa tcgccagtcg atgacaggtc gacaatagcc ccgacgagat cccgtttttt
    56581 tgttcctgct gcccgtaata ctgcccaagg agaaaccgca gctcggtgcc ggtcattttt
    56641 ttctgaaatt cgggccgcag cagcaccgcc gtcaaaaatc gatcctgatc gagcagcact
    56701 tgttcatcaa acgccatgtc ggtgacgcga atcgtccctt catcgcgcag cgcaccgtcg
    56761 tcgccaaaga tcggaacttc atggaccgga ttgcgatgcc cgtcgagcga gcggtgtcca
    56821 tcgtgataac agaccgcatc tgtcgtcgac aaataggcaa cccagccggt ttccaaaagc
    56881 ggattgagcc acgtctggtg ctgtcccgac accgtcacag gaccagcgat cgccaccgtc
    56941 aggggacaac cggcaccgat cgccgcgtcg agcagcgtat aaattcgccg cagataggcg
    57001 ccaccaaacg ctggcaggca gtgcaaaaac agctcgtcga gcgattccac ttcgtcgacg
    57061 cgacgggtca gcacggtgcg gcgctgggcg tcatcacttc ccgacggagc cgcagtgtcg
    57121 gaggcatttt ggcaaggata gcggggggtc gtcatcggcg cgcactatag gcaaaaagcc
    57181 gcgccggtgg aacgcgaaag cgttacttgt ttgtcgacgg tgggagccga atggacagcc
    57241 ggtcgccaag ctgggcaaac agccgcagcc atgtaaacgt ccgctgggac tcgctgctgc
    57301 tctgctgcga gcgctgcact ttttcgcgat acagcgtctg ctggagttca taaaaagcgt
    57361 tctgggtccg ccacagatcg acggtgtacg gcggtgttgt cgaaagaacc accgcgtcga
    57421 gcagcgcttg caacaccttg gtcgaatcgg gcagctcgcg cagcttgtcg gacagtcgct
    57481 gcagcaagcc ctgaagcgca aaactgaccg ccgcttggtc cagccgcaca ccgctgcgct
    57541 gcacttcgtc gagcagccgc cgaatttcgc caaaatcggg acttgcggcg ccaagctgag
    57601 ccaaaattgc cgtttgaagc gactgttccg ccgccgtctt cagcgccggt ggcagcggcg
    57661 cgtgcaggtc cgtcaaaaag cgcaagagcg gcgcgtgggt gtcatagatt ttccgcagct
    57721 cgtcgtcgac ttccgcccgc gtattgcgca acagctcgtc gagaattcgc cgctgatcgt
    57781 cgcgaaagag ctcgcgcagc gaataggtcg cctgcccaaa atagcgatcg atcattcgca
    57841 gcaccgtcgg caaatcggct cgttcaaagg gctcgacgag gtttttgcgc agctcaccca
    57901 gaaagtcctc gctgcgcgtc tcgacaacac cgccgacgag gttgtgatcg ccaaagtgca
    57961 gcaccgcaaa gcttagatcc gccgactctt gcgtcacggg ggtcgccacc cgcgcccgcc
    58021 caagctgaag ggaaagtcgc cccgagcgat gcaggtgctg ctgacgcggc tcgatctgat
    58081 agccatagat gctgatcgga tcttcgtagc gctggaacag cgacgtgatc gcgtagtggg
    58141 caaatgcttt ggccagatcg actttccgcg gcctgacctg gctttcgtac agttgccgcc
    58201 cgttgcccac cgctgcaaca ttgctttccg cagtcgccag cacgtcgaga aaaaccggtt
    58261 ccagatcgac tttcagcgcg gtctgggcca gctgaatcgc ccgcgccgcg tagagcaagt
    58321 tttgcagtgg ctcaacgccg gagatgtcgt cgaaaaacca cccgcagctg gtgtacatca
    58381 gaagcaggtg ccgctgcatt tcgagcagct gaaacacccg cgccgtctcg tcgggggtca
    58441 gcggacgctt ggcttggttg cgcaaaaagc gctcgcgcac ctcgggtcgt ccgtcgatgg
    58501 tcacgtcgat ataggcatct cgcgcccgcc acggatcaga caacagcgtc gctgtcgcct
    58561 gttcgtaaag cggtgccaac cgatcgcgca acagatcgag tgccgtccgc agaggtgtcc
    58621 gccagcgctg gtgccagccc ggactgccac cgctgttaca accacaatcc gatgcccagc
    58681 gctcgatgcc gtgtgcgcag ctccacgcgg ttcgctcgca aatttcgact tcatgggtcg
    58741 gtggatattg ggcgagaaat tccccgtaat tggtcaggcg aaccccgtcg gtgcgttcga
    58801 tcgtgcgcag cgcaaaagcc agcgccattt cgccatattt gtggtgatgc ccgtaggttt
    58861 ctccgtcggt cgcaatgtgt acaagctgcg ctctgtcctg gccgttccga aagccacgca
    58921 gaagccgctc cgccagcttg tcgccttggt ttagcaggtg ttcgaatgcc accgcccgcg
    58981 aggtctgtcc gtcgtaaaaa aacaccgcaa tccgccgccc cgacggcagc accacgtcat
    59041 acggcatcga ggtgtcgact tgcccgccgc ttacgtcgcg atagctcgac tcaccgcgtc
    59101 gccgcacgcc tttggcttga tgcggcgcca agatggtaaa tcgaatcccc tctttcgcga
    59161 gcgcttcgag gctggccacg tcgaccgccg tttccgcgag ccacattcct tccggcagac
    59221 gaccaaagcg gaacgcaaag tcacgcacgc cccacaccac ctgcgtccgc tgatcgcgcg
    59281 ggctgcacag cggcaggatc gcgtggttat acacctgggc cagcgccgag ccgtgccccg
    59341 aaaagcgcag catgctctgc ttgtccgcca gcaaaatcag atcatagacc cgtcgcgcat
    59401 gcaccgacat ccaggacagc agcgtcgggc caaagttgaa gctgacccgt tcgtaattgc
    59461 tgacgatgct ggtgattttg cccttgtcgt cgaggatgcg tgcataagcg ttgggctcgt
    59521 agcactcggc gcagatccgc tcgttccagt cgtgatacgg aaacgccgag tcctgcactt
    59581 cgatggtatc gagccacggg ttttcacgcg gcggctggta gaagtggccg tggatgcaga
    59641 cgagacgcgg ggcggcattc atagagggga agtcctgccc cgatgctatc accagtcgcg
    59701 ccgccgtcgc tttgcggtat tctccgc
//
LOCUS       DAORGN010000006        57550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_171_length_57550_cov_5.519158, whole genome shotgun sequence.
ACCESSION   DAORGN010000006 DAORGN010000000
VERSION     DAORGN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 57550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57550
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_57550_cov_5.519158"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PKL17_01395"
     CDS             <1..963
                     /locus_tag="PKL17_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK43406.1"
                     /translation="VAPTPKEVIWRKNKARLYRYTRSEKVTKRTPVLCIMPLINRAYI
                     LDLRPGASFVAYLLSQGHDVFLLDWGEWNDEDRSLNLDLLLTQYMARAVRIVAKEAGG
                     PITMLGYCIGGALATCFVAQHQESLIKNLILLTTPIDFTDAGPFGVWTRPDVFPLELL
                     TSVFPAVPGGYPDVGSKLLQPLPAGMGQFVRLEEKLRESRFDVHGWQAMFRWVNEGVA
                     FPSGCYRQWIGDFYQRNKLVRGELVINGQNVLLSNIRCPLLNVVASTDIIAPRPTTNA
                     ILSHVSSTDKQEVVVKGGHVGIVVGKAAALDLWPKVSSWLAARD"
     gene            complement(1001..4171)
                     /locus_tag="PKL17_01400"
     CDS             complement(1001..4171)
                     /locus_tag="PKL17_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNK43407.1"
                     /translation="MRSAPFVPHDDLDDDDEESGPLSAESYLSEAHHSEPVRLSAQRR
                     VQAGADPQPVRAPAFTAPPSELLDSRQRREVVIPPRPRKAARLPVEASVPKVIDRETR
                     REAPRLDTRPSPPQDVSLPTANLPREPVAIPRREPIAIPPREPIAVPPRDKVTFSDSA
                     AVAPPADKDAAALEPAPAKARATAREGAVREAVAARRAAKAGKAEPPPPLVLSSRRRE
                     AIGIALLSVALFVSLATLSLMFGDGQLMGPVGQAVASALYALLGIGTIGPALLLFVFA
                     VKLLRTQKLTLAKKLVGGGTAAWLLTMTLAHLAGGRFRIAGMPPGGALGEYTAEALIA
                     MLGTAGTVLLLGMGLLLSVTMLTPLTTRTLGAKLHDAVRPQLRQIKEKLLSLLPKKRL
                     DDGPPIALPGSGKPSSGVPRQVRDYGDDRDQTNLPAKKKPEQSEDEPAPVIVARRPAP
                     KPDEAVPGLGIHGEPTKLLTEEPTRKTDAPATADGPAVVIAAQVPERADATEKMDGPP
                     SHMPLPDDEPAAALATPELPDVVIPSEPDDESSLGIVQATRKPEPRKPAGQANYIKLR
                     PYEPPPIDLFDFAEHPQHELDRKAMLDLAQRLEGTLGDYTVKGRVTAIHPGPVVTMYE
                     FVPAPGIKLARITSLSSDLAMALEALRVRIVAPIPGKAAVGIEVPNRSRETVYLKELL
                     ADDSFRHAKSKLTMALGKDIAGIPVSVDLAKMPHLLVAGTTGSGKSVSVNTMVCSLLM
                     NASPDEVKMIMIDPKMLELSIYEGIPHLLLPVVTDPKKAALALRWAVEEMDRRYDLIS
                     KAGVRDIGGFNKKVEKHQEITASGQLSLGVDPDDFSGPSGEPVQKLPFIVVLIDEFAD
                     LMMVAGKEVEICVARIAQKARAAGIHLILATQRPSVDVITGLIKANFPSRIAFQVASR
                     VDSRTILDQQGAENLLGMGDMLFTDRGQALRRIHGALITDGEVHRVVEHLKQLGKPIY
                     DMDILKPRGDDEQEGGEEEVVDELYDQAVRIVSEGRQISISMLQRKMRIGYNRSARMI
                     ERMERDSIVGPADGAKGVREVLIHNH"
     gene            4303..5649
                     /locus_tag="PKL17_01405"
     CDS             4303..5649
                     /locus_tag="PKL17_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK43408.1"
                     /translation="MSLAPSPSQFQNLLLAPKLLSDPRKPIESLSARFGRIFRTRTMS
                     ASGLREMVWLLGASHNERILAPAHRDDFTWYEGYGFTMEPLLGRDILFLLDDQPELGK
                     EGAHRRRHRLLIPAFQPRLDGQYLPAMAQIMGHHLGRLPLGTAVDLAATVKQVTFHIV
                     ASLLFGMPADDISLLLHDFEDVGLGLFSLVHLRIPGLPFYRADRARRKLAHYVRQKIQ
                     AFRQKQMPAPTFLAQLLDAEGEDGERLSDETLVSEMLAFLFAGYDTTASMLTSILVAL
                     ADHPEVRTRVQHELATVDVEQLRAGALPELPYLDAVLLEVERLFPPLTFAMRGVLRDF
                     EIDGYTVRRGDNVAYSAYFTGRDPELFPDPLRFWPDRFAAQKPRPYAMVGFGAGHRPC
                     IGKRFSLLEMRLFVALLFRQFDVQFAPDQSTAVFFNPTMQRRDGYFATLSLHRAAA"
     gene            5671..7242
                     /gene="crtI"
                     /locus_tag="PKL17_01410"
     CDS             5671..7242
                     /gene="crtI"
                     /locus_tag="PKL17_01410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNK43409.1"
                     /translation="MALALQNPSQGNRVVVIGGGVGGLATAIRLLAAGYSVTVLERAQ
                     TPGGKMRQVEVGGLPFDGGPSVLTLPWVLDELCQVAGVRRSDVIELAPLDPLCRHFFA
                     DGTQLDLYNDDPPDGRRSTDASWARSSEEIRKVIGAKAAEEYGRFRRHAAKIYSAVER
                     PFLQSPIPSNPLGLVFHNRISDVMGLWKIDARRTLWQALCSFFSDERLRILFARYATY
                     SGADPFLAPGTLAVIPHVEQGFGVFAVKGGMYRVATALAELVLRLGGCIRYGADVERL
                     ILDDKGKQVTAVQVAGERMFADVFVANCDVPQLTLRMLDGTKVGAQLSRLYDKLPPSL
                     SAVLHLAVADQAAQLPLCHHNVFFTRDYRQEFVELVDGPPTDPTVYLCNPDFAQDRQR
                     WFFLTNSPALPRDGEPSKQSPWTEPLVQTCRQRVVEKLARHGISYDRHVRAEGSVTPQ
                     DFARLFPASRGAIYGAAASSKIAAFRRPPNEVKGLANLFCVGGSTHPGAGVPMVMLSA
                     QIVAGLCQKRFPRPR"
     gene            7354..7632
                     /locus_tag="PKL17_01415"
     CDS             7354..7632
                     /locus_tag="PKL17_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNK43410.1"
                     /translation="MAQLVHCRKLGKELPGLDFVPFDDALGERLQREVSAQAWQMWLE
                     HSKMLINEYRLDLITQQAFDFLHRRCEEFFFGEGSELPKEFVAPEHKH"
     gene            7651..8061
                     /locus_tag="PKL17_01420"
     CDS             7651..8061
                     /locus_tag="PKL17_01420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNK43411.1"
                     /translation="MGFRTSVQVRFGDVDHAGIVYYPHFFIYFHEAFEDFFNDAGISY
                     VQLLDERRIGFPTVHVETDYKAPLRYGDRLDIEVSVEKLSNRSMVMCYEGYRHRDGKH
                     SVTCRITTVCVDMNTFSSREIPQDLRELFERFRR"
     gene            8058..8918
                     /locus_tag="PKL17_01425"
     CDS             8058..8918
                     /locus_tag="PKL17_01425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNK43412.1"
                     /translation="MSGPRKAGMVAVLRPQLAEDGADFALLLVRRSQRASFMANAYVF
                     PGGRVDAADAEHDPEWPSRYAAARELFEEAGLQAATPKSLVRFAQWITPSAEPKRFDA
                     DCYLLSIDATTARHGDAVQVDGHEVFDPLWLTPREALARYQAGDLNLPPPTVCTIEEL
                     EAERLSALSILRQRGELPTDATLQTASQLVFALIASCAASRKPYPLLPKLIADPSGTG
                     IAIVMPWDSDFAAQPGDGTSFAAMAENGPPVPRRISRCILHMAKADETYAVQPGRTES
                     VRWQIERLPG"
     gene            8925..10214
                     /locus_tag="PKL17_01430"
     CDS             8925..10214
                     /locus_tag="PKL17_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK43413.1"
                     /translation="MRILLLNQYYHPDIAATAQLCTDWAESLASQGHDVTVLCGSGRY
                     RTPHLGLVPQQTDDLPAEQTRRGVHIVRVPVEDAPPPIGAAPSLRERFDKLFGRLSRY
                     GQFVSQATLRLRRVPRPDVVVALTTPPLVGGLGLLAKKLHGAKLVLWVQDVYPDLLPA
                     MGLMDAGSAPYLCLGQFARLLYRSADRIIALDEAMAERLTNAGADSSQLTVIDHFADA
                     QEIRPQPRGPSKIRQLVGLSDEFVVCYAGNHGRGHDFDTILQALQAQADSPPGMPPIH
                     WLFVGDGDEKAGLVARLPTAMRPYVHFLPPQARHELPDVLAAGDVGLVAVKAAMQGLL
                     APSKLYGLLAAGRVIAYIGPQEGRIPSLLRECEIGISVRNGQGPELLQALWALAADSV
                     RWKRMAKLARQLAETRFDKPIAMAQHERLLFDVVGSR"
     gene            10214..11611
                     /locus_tag="PKL17_01435"
     CDS             10214..11611
                     /locus_tag="PKL17_01435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNK43414.1"
                     /translation="MRAVGLGLLLSLLLALLLTPLLRRAAVWLKALDPVSARKLLRPS
                     QVPRLGGVAIALSFYLTVVVLWSTGSVVARATLQQGTHVLPILLGGIPILLLGIVDDL
                     RGMRALPKLLVEIAVALGLYWQGLRVLGTSGPSGPIELSQLLSAVVTVAWVVGVVNAL
                     NLIDGLDGLASGVAVIALGTTTVAALLRGELLLAFLTGSLAGATLGFLRYNFNPASIF
                     MGDSGSLFLGYLLATSSIWSVRKSATAVLVVFPVVALGLPLLDTGLTISRRFLAGRPV
                     MQADRDHVHHRLLLKGLPVRRAVLLLYGVCLVFSGLSLLMVLGSPWAARLGLALSVGF
                     ALSLGYWFGYLRSGPQGLWAALTRRGRTQKLLARLDVLQAELQAVQSLVALEQVVAEF
                     GDLLGVTLRLDRQTDAVEPGSYPIMSSGKQGKLLAVLRVSPPEHESNPDERTLIGLFC
                     DALAPTLLRVLSTES"
     gene            complement(11612..13165)
                     /gene="gpmI"
                     /locus_tag="PKL17_01440"
     CDS             complement(11612..13165)
                     /gene="gpmI"
                     /locus_tag="PKL17_01440"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNK43415.1"
                     /translation="MKPVVLIVLDGLGHREEPAANAVELAHKSTWPRLLAQYPKTLLH
                     CSGLSVGLPEGQMGNSEVGHTILGGGRIVYQDLVRISKAIADGDFFENPELVAAVDAV
                     KASGGTLHLLGLNSPGGIHSTLEHGYALCELAKRRGVSKVAWHAFLDGRDTPPQSAAG
                     FLRDVEAKLAAIGVGRVASLAGRFYSMDRDNRWERVARGYRLLVRGEGRPEPASAGAA
                     EAILAAYARGETDEFIQPIVLTENGRAVATIQDGDAVVFWNFRADRAREISRALTEEK
                     FDAFDRGRPPRLARYVCMTQYDQKLGLPVAFAPQSLHNNLGEYLSARDFRQFRTAETE
                     KYAHVTFFFNGGIEAPYPHEDRRLVPSRRDIATYDLAPAMSAADVTDGAVTAIESGQY
                     GFILINFANPDMVGHTGVLPAAVSAVETIDACLGRICDAAKKSGAAVLITADHGNCEQ
                     MVDPDTGQPHTAHTTNPVPFIVVDDSLRGQRLREGGLADVAPTVLDIMGLPVPPEMTG
                     QSLLVKSRA"
     gene            complement(13215..13613)
                     /locus_tag="PKL17_01445"
     CDS             complement(13215..13613)
                     /locus_tag="PKL17_01445"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNK43416.1"
                     /translation="MKKVIRVVAAVLERDGRYLITQRRDSAVLPLQWEFPGGKVEAGE
                     NDEDALRRELSERLDADFEIGKKIGEKHHIYDGYEVILALYAAKLIPGRPIRAKRVRD
                     FRWVASSEFGQYQFPDADQRTMDQLLGIRR"
     gene            13825..14886
                     /locus_tag="PKL17_01450"
     CDS             13825..14886
                     /locus_tag="PKL17_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK43417.1"
                     /translation="MGLMDFVKGGVRELAIARPDSAKGMLVYKHPDPTIPNKAQLTVG
                     TDEVALFFKDGQFVGQIGAGRHTLESSNIMFLNQLVDKFTGGNVFKAEVWFITIREVA
                     GFKFGGRIGDVEDPKSGLAIGVMVHGEYSLQATDPTQVISFFGQRSWANDEEFVGWFR
                     QLLLKTIRDRIAKLLVAKQLPLLNITSGALTEEIEAEVIEGLKPHMQGYGMRVVRLGN
                     FVVSIKEEDEQTLKSMYKDAAQIRMAGGLGGYQQFAAGKAIMGAGEGMARGGDGGGGG
                     GGMLAGAGLGVGMAMANMFAQSTRPAAEAPAPAQAAPAAPAQPSLQDRLKKLKMLKEE
                     GLIDDQEYAAKRAEILKDI"
     gene            complement(14947..16929)
                     /locus_tag="PKL17_01455"
     CDS             complement(14947..16929)
                     /locus_tag="PKL17_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43418.1"
                     /translation="MRTRTLAFPLLGSFLLAAPLLASCTVHVDLTPLPEPPLAAGCNP
                     LGGGEKEDCVTPFPSNYYRSPDDKGVMRISFPPGVLPASAKGVALDPALLLGRDGFSP
                     AVQLVAYFPATIDGSNLPAPRHEEDSIKVSSPVQLFDFETGQRIPLFAELDRNATDGD
                     RQALLIHPQVRLRPKARYVVALSGLRGSDGQPLTPLSGFAAIRNGDVEPGSIRARLAE
                     TYKELFPFLEKQGIAKKSLQLAWDFTTGDDSQVTGQLVKMRDQALAKLTPTDPPEPPS
                     PLFTIDKVENAPRDPLLRQVIGSFRATSFLSDDQNGRLLKDESGTPKARGYGRFPLVV
                     HIPKCAQTATGPLPVMIYGHGLFGGALGEMDSGYQREIVNRLCMVQVGTDWIGLTEAD
                     GLYVAGQVMSNFNNFVQITDRLQQAHVNFAVLARLVKDGSLGQIPELRVNGKVIVDSS
                     QVFYYGISNGGIQGLTFLALSPDVKRGALNVPGGFWSLMMWRSGDFQDLVPLLSSNYH
                     DALDRQLLIALSQQLWDYTDPATYAPYVVKAPLPGTGSAKQVLYQEGLGDGQVPNLAT
                     RMVVRTMGIPLLNQPVETVWGIPQASGPVPSAYVQFDVGQKPRPGDDNVPPKDNPVHE
                     AIRRTEAAKAQLQAFLKDGGVVTDTCAGKPCVFPKN"
     gene            complement(16983..18386)
                     /locus_tag="PKL17_01460"
     CDS             complement(16983..18386)
                     /locus_tag="PKL17_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43419.1"
                     /translation="MSLRAFVVRQKLSLRLSQLHRGDESLTPAITWHNLLCRLGVPLP
                     LGLVHDFGLLLSTPASQLRVGLDVPPPPALRETTERYRTLLKTLLAVPSVVDIAANRP
                     SDEVCAVLLAKLLRPLVPDLHSMELATIELSPKAIAESALPSPAEQTHELARLARVCT
                     DLVAKSVPLLLRCELLDAQAVRLLHLSEGSTQLLSRASDDRVDLLELESLFDADDLRD
                     TVRFSLDLLPSVLEAQHVSGAQSYPTGGYAGLLHRGGLDALLPSELASDDDLFYTRFA
                     LSELLYLGRERQVEAPHPLSYVLVDCSPSMRGLRQVFARGFALSLVQRLLRSKQPVQV
                     RFFDGRLHDALRADRSPRRILPYLLGFRSNRGRNYKRVFSDLVRELSTMTAGNKARVT
                     LFLVTHGECHIQSELVAEVARRARIHAVFVRPSSDLRLDYLAKLHRYHVISDDALHSE
                     ATRKQQSLAILQGVTPS"
     gene            18444..19019
                     /locus_tag="PKL17_01465"
     CDS             18444..19019
                     /locus_tag="PKL17_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNK43420.1"
                     /translation="MALRKIAQIGHPVLRVLARPLSREELLSQAGQSLIDDLIATMRD
                     ADGAGLAAPQMYESVRVCVIEVGQNARYPYKPKIPLTVLVNPVLTPLDARTFSNYEGC
                     LSVPNLRGRVDRFCEVRVQAWDRFGNELDFVARGISAGTYQHEVDHLDATIFVDRVKD
                     TRSLCTWQEFERHHREAFIKEVQAIVAEFGS"
     gene            complement(19105..21267)
                     /locus_tag="PKL17_01470"
     CDS             complement(19105..21267)
                     /locus_tag="PKL17_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK43421.1"
                     /translation="MQSRSTHFTTCNLCEAMCGLSVTVEGGRVSDIRGDRDDGFSRGH
                     ICPKAAALRETYEDPDRIRQPLRRTPSGFVPVGWDEALAEIADRLDSIRRRDGRNAVA
                     VYAGNPTVHNLHALLGYAGFLRALGTKNRFDANSQDANPRLLTSLLLYGEQTVIPVPD
                     VDRTDFMLMLGANPAASGGSLMSLGDVKHRIRGIRERGGKLVLIDPRRSETSEWASEH
                     HFIRPGGDAALLLAMLHVLFAEQRVHLADVRKRAHGIEQIERLVRDFPPASVEAAVGI
                     PQDTIARLARDFAAARHAVAYGRIGTCQQELGTVANWLIDVLNLCTGNLDREGGAMFP
                     RPAFDLGALGRKVVENHHGRWKSRVRGLPEVGGNLPAAVLAEEIETKGPGQIRALVCV
                     LGNPVMSVPSSARLDRALASLEFMVSLDYYINETSRHAHFILPGRHALERSQYDVVFH
                     ALQVRNTAKYSLPVIEPSENTRTDWDILYDLSMRLGGVRTGFKVVDRALQLAHRLGLR
                     PTPEHIVDLALRSGRYGNGFVPGRPGLSLAQLKSSPHGIDLGALSPCLSDRIRTRDGK
                     IDLAPQILVDDVTRVKTWLLQAPKQGLLLIGRRQLRTNNSWMHNCPSLVKGPDRSHLI
                     VHPADAERLSLRDGHKVKVTSRVGQVEVTLSVSDEVMPGVVSLPHGFGHQQAAATLRV
                     AGALPGQNANELTDDHLIDPLSGTAALSGVPVEVTPIP"
     gene            complement(21286..22467)
                     /locus_tag="PKL17_01475"
     CDS             complement(21286..22467)
                     /locus_tag="PKL17_01475"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43422.1"
                     /translation="MTTDSGARNRAVLLQELGRRLTREFLAKDEIIRLLLLSVVAGEH
                     MLIVGPPGTAKSALVRTLAQSLDARYFEYLLTRFSEPNELLGPVDIQAFREGSFRRRT
                     EQMLPEAEIAFLDEIFKASSAILNSLLGLLNERRVQIGAERVDVPLLALFAASNEVPS
                     DDSLAALLDRFLLRVRSQNLEGFHFQQLLRIGLEHEKRLLGGRSTAPSGPSGPPLLSA
                     KDILQLRRELAGRLQFSDEFLSEYKALTAQIRSEGIFLSDRRLIKMLKLCAASALCDG
                     RTEADVSDLFVLKYIWNTVEQADLLAGLVDPVLRRHRQHKAGPRDEQGRRAMLDELQT
                     LRLGLQRAGELSDVQLFAKLRSLGELRAALQSDGSEESLRAVSEIDSLLDSVVSQSPV
                     G"
     gene            complement(22464..23561)
                     /locus_tag="PKL17_01480"
     CDS             complement(22464..23561)
                     /locus_tag="PKL17_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43423.1"
                     /translation="MKDRDFAAAFLARFAYPLASGGVVSVGMPLLQKGADRLLRAAPE
                     LHQTAVGQSLHKAQKQQLGEIGIVTEPRPLADDPHSLLLLSAIHDALFLFHPRGRTLS
                     QSRRDGLIQQVRLQLIAAAESLPEPSLETAQAADFVPKLTARYSLLSGLWALRPKSGE
                     PLPQPQASIEHDVLALPIDKAEKTLWHYLGLGSPLTQLLRPPPAPLLGPELVPWLQVA
                     RIARLFVTVQLRHGSTQALSRIGRSVEAALEANPDELDQGSLRTLIGLFALFHLRVAT
                     ADGPPLAVTDQQPLRESLSLFAALASVDPALVFPPDIGTSGAIYEKVQRHRRLCQEVA
                     RPESLQRQKELCKKRCGPRTLTTNPHTMDNR"
     gene            complement(23558..25147)
                     /locus_tag="PKL17_01485"
     CDS             complement(23558..25147)
                     /locus_tag="PKL17_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43424.1"
                     /translation="MPMLEPVAKNQLSFRIAGLFQDGRGVVVGTEAVVVLLSIERVVS
                     LTRLLSEELPLDDLVPTLRIELCKNPLGSTLYLLRFQCRDSLLLDRVARLARLLSAPL
                     CVGAGRHFVAYRDDAAPLGYDATTPLAEPGDLAIYTPTLVQPCRKIKELSLRDLVEQL
                     QLLTLPGGLLRELAQPQSPDDSPAVSEPIDEIYLTCGTTLLPRLVRYLWQTGASGDLL
                     IPDPLPSPEGLPSDRIFRAPIVRLVTPPLSLLLRLLTLPSVRLLRREGSNFLIDLGHA
                     HPLRLTSMQSLFPAADLYLFLAGRRGSLRAKSPSKTPLGRFVTPQIAPPGEDDAAAQP
                     LQAVRLGSSTSPPPPLKLTLRLVAAHSALQDPLSATLIPWHRLATLQRLLGLLSGEAI
                     FSLHAVGLPAGLFCFGSAAVQHLCAGQLFAEVSPQVFVPARTRLLPDLPPSQLRALVQ
                     HGDDHLVVFLPAETQPIAVPKSLASPLSLRLLQSLATKLPDALIEPAALRDSFPVLHC
                     EPISFFSTLPLWGLASDPERG"
     gene            25186..27492
                     /locus_tag="PKL17_01490"
     CDS             25186..27492
                     /locus_tag="PKL17_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNK43425.1"
                     /translation="MVQTARLCCFRRRFGLLTWLLGGFLAACSAPPSVGRVAAPVADV
                     LPDHYLLTVDAPMSGATQKSLRDKVAQLGLAVEYEFSDRVGLAGGLSVRGELEAIQAL
                     QSAFPSLRVASAVLRYPAGTTCGNGSCETDEAASSDRGSLCSLDCGAQPLRPVRDELT
                     NSFFVRQVGADQVWPKSQGAGVSVCIIDTGFDRGAMSTHPDRPQALGEGYSFVRQSAD
                     YADVSQHGTHVAGIVAAAKNGTGSVGVAPQATVRNFQVFRLKNGSPVASDADVIAALD
                     AAIGAGCQIVNLSLGGASDSEAEHAAIAHAYQSGILVVAAAGNGEDAAHGVVGTAQSN
                     FPAAYPESFTVGAVSRSDTLASFSGTGPAVGIAAPGVGMYSTVPVGTGQREVSASFAQ
                     NGRLLAVDASLPSGSSGTSLPATRVVSCGYGSQSDIDACQPAGKVALIQRGPGGPGET
                     AIPFYDKIRVARQGGALAVVLYNHRYGDPSTAGALLENITIGGGQPVMVLTLSAGDGE
                     ALAEKATQGGLTLSATSSPSDYAVFDGTSMAAPVVSGVAALVWSANKSLGNVGLRQLL
                     SESAVDLGAPGRDDQFGAGRIDALAAMTAGSPRGLCGDGVRDRASEMCDGASRDGTSC
                     DDLGYDGVLSGSPGCNASCTGLTQGTCACVPGRTPFAVTLGVDENAQVNGGLTGTRFR
                     YHVELAGKPVAAARIKVTIGKNGRNLVSYRTPATDANGNSQDFVPYDFTGLSAGDYQV
                     MPTVSKGDGRCRDDQPTQPAQVTVHIRS"
     gene            complement(27493..28776)
                     /gene="serS"
                     /locus_tag="PKL17_01495"
     CDS             complement(27493..28776)
                     /gene="serS"
                     /locus_tag="PKL17_01495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNK43426.1"
                     /translation="MLDAKILRERPNEVLSIYRERLFDDAAAELAQTALGLDDKRRNL
                     LQAADELKSRRNSASQLIGREKDAEKRAALIKDMESVKTDIAGLDAQLSETEAQLDNL
                     LLQLPNLPDSEVPTGRSDAENQVVRTEGKRFEFGFAPKPHWEIGESLGIIDFERGVKI
                     SGSRFYILKGAGAALQRALITWMLDVHIDQGYTEVYPPYMVKELCLYGTGNLPKFGDN
                     LYRDAEEDFYFVPTAEVPVTNLYRDEILENEQLPIRHVAYTACFRREKMAAGRDTRGM
                     KRGHQFDKVEMVQFVRPEDSKQALRSLLSDAEEILKQLELPYRVIQMCTGDLSFAACE
                     KFDLEVFSPGVNEWLEVSSCSNFGDFQARRAQIRYRPGKDKKDKPRLVHTLNGSGLAL
                     PRTVIAILENYQQADGSIRVPAVLRPYLRGREFIK"
     gene            28871..30598
                     /locus_tag="PKL17_01500"
     CDS             28871..30598
                     /locus_tag="PKL17_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43427.1"
                     /translation="MKTHPVRGILAASLFGLGLLTVAGGCAGSLQRVPLSANLRPSGP
                     PSVRKAQDLGSLPLPGAGPLSLAHADDLFTPGEYVALYGENLTPGSQVVLSGRAAAVV
                     GAIDGGLLIQVPRGISTGRQVLRIDNGLGTAEVTVSTAFYAFGGDAAGNGLRIRRLGP
                     EGDQPEMFDDKPLDIAFPLSRYQALSYDGALLFALAEPATDEAPESSSGEAIPVGTVV
                     CDLMVVHMGAKHGPQEVTRVPLSLVGKPTGLAMGPAGQLVVLQPRQITVVDVSIPQKP
                     QPVVVLPLWPAEQKRELVDAEFLGDGRLLAVLEAYANQIHLIDVGDPRQPRLVSGVSL
                     SPEYEQPFSIDLAPGPDPSSMWVLQGPNLRLAGKRLLDGLSGAWRDAKALDVKGAAKT
                     LGQTAVGAAMLPSEPLTRVQLVQLVGGQLQVVKSLPLPSEIFPFFIQPDYRGSLFVSG
                     VNRKNQFSSIDASLDGVRRLLGAIKDTTQLGVILKMGTEDGSVATAVQGVAMYYDLAL
                     LPSGQLMTSTIRLGPGYIPPRVTLDWGFEVPGKSFAKLREVANTGFKITDVVRRLLPP
                     YRYERIGVQ"
     gene            30667..31542
                     /locus_tag="PKL17_01505"
     CDS             30667..31542
                     /locus_tag="PKL17_01505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNK43428.1"
                     /translation="MPTVDFDFQRYVARKKGARAAQVREGAAYAYPGDVRLLRTLDRL
                     RPVELALLEAGRLWQSVARAELLGPAVRAVAPRFGQVATAAKRCADILHIDVPTIYIA
                     PRPSGVLTLGTEAETYIVLSQGLLDSLSPDELLDVLGRECGRIQNGHVPFRTALHYLQ
                     HNASTMTRWAVRPATLTLLAWARRADITADRAGVLCARSVDVSLVAMQKAFPEDDSRE
                     LARRYRAIQRFVGSLYYRTVVGQDAEDGESTEDCDAAVAQILDDKEVPPRTARGAAAA
                     ASGESSGEAGQGGSR"
     gene            31539..33335
                     /locus_tag="PKL17_01510"
     CDS             31539..33335
                     /locus_tag="PKL17_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNK43429.1"
                     /translation="MSGLTKQHRQRKDELLSALREVARLADETGMHTLTSELLRERIP
                     KLEEERFVLVVLGEFNHGKSTFVNALLGASVLPAGITPTTATINHIVYADKPCAKAVL
                     LDETQRVVDPKSLNEWVTIEGSKAEKTRYVEVGWPADILRDRVTLVDTPGVNDINEAR
                     AEITYNYIPRADAVLFLLDSSQVLKQSERSFLEQRILRRSRDKLLFVIGKSDLLSPEE
                     RAEATRYVRDNLQPLLGAEPMIFPVSARLQLAGRSDESGIPELKSYLQHYLADERGRV
                     LLDNASSDGQRLCAYLRQNLGIKARSLTLSLEELEARIARVQKELSGKQQTMRQLQAR
                     IVAESDAIKARIRLDLEEFVAAFSSALPGEIDKASSDDVRKYLQLFLQDTFKQWAEQE
                     GDRVASELERLAEQIIQITNENVQQAMATLAAELGPTDTRVDLQVDTLKYDVGIFALG
                     ALGTGIFLFVNSFVGGLLTLAAPIIAVLIHSRMADEIKRQAKQQAPQVVQTAAQAMLP
                     RFQQIVDDFAARLADFVTAAGQALHRGISEMLDRALAERKAQGLDVAVRQREIDAQVT
                     ELHQVEQRLSTIREALWAPAEEGDGADAVDPQ"
     gene            33332..34117
                     /gene="gloB"
                     /locus_tag="PKL17_01515"
     CDS             33332..34117
                     /gene="gloB"
                     /locus_tag="PKL17_01515"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNK43430.1"
                     /translation="MITVELVPCYSDNYAYLLSHPDSADVVVVDACEYAPIAAALGGR
                     RLSAILSTHHHLDHVGGNEEFAAQFPGLPILGHSQELVDGRIPRQTAGLADGESFSLL
                     GSRVVALHVPGHTLTAVAYHFTDEGIVFTGDTLFGAGCGRLFEGTPVQMHASLSRLAA
                     LTPSTKVYSGHEYLARNLGFARSVDPGNQATQDREQDCQTRRARHEPTEPSSIALERS
                     TNPFLRTSEPAVQAAVQSRPVETEDALSAVEVFARLRRWRNTF"
     gene            34157..36844
                     /locus_tag="PKL17_01520"
     CDS             34157..36844
                     /locus_tag="PKL17_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNK43431.1"
                     /translation="MIRRFAEISAHDLPKVGGKGANLGVLTRAGFPVPPGFCITTDGF
                     AAFVAGCAELPTLFADLDALPVDDVEAVRVVGAKVRAALLAVPLPAAVVDGAVAAWKV
                     IGDGYAYAVRSSATAEDLPGASFAGQQDTYLNIRGEAALIDAIRRCFVSLFTDRAILY
                     RQKNRFGHRGIALSVVVQRMVFPDAAGILFTADPVSGHRGTIAIDAGFGLGEALVSGL
                     VTADLYRIDKATGAVKEVRVGDKAIAIRALPAGGTVTEPLSPSQRQARVLDDASLRKL
                     WDLARRVESHYDGVPQDVEWCLECDDLFVVQARPITSLYPLPTPTPGRGLRVYFGFGH
                     AQNMIAPMSPLGLDIWRTVFPIGKRELTEVIEEPTVMVTAGGRLYIDVTAPLSTPRLR
                     TALLGALGAIYPDIARLLAELAQRPEIATADSPSLRSFVPLWTLLGGVPPRLLKELLW
                     NDPQRLRTELEACIDEKLSHFRRETEQLAPGLARLARLRSLMSSMFGILQHFVPRFGS
                     AMLSLRLLTQRFADTVHAADVGAIQRGVYGNVTTEMDLLVGDLTDLVRPHPELGAALR
                     LAGSQPFSRESLAGLPGGAAFVEALDQFLHRFGMRGPGEIDIARPRYRDQPGLLFSSI
                     IGGLSGSQPGAHRAHFARLQKEGDMAGERLIAAAGGPLARRLVARQVGCVRYGLGLRE
                     HPKYFLVRCLDVLRTEVLFAARELVAAKRLDSVDDVWLLRYAELLQLLQKPTLDLRHV
                     VAQRRSDYVRYAQLQPPLVMTSDGEIPRLPPPKDLPQGALAGLGASAGVVEGLARVVL
                     DPAQDVLHAGEILVAPYTDPGWTPLFVHAKGLVCEVGGMMTHGSVVAREYGIPAVVGV
                     SSATRLIRSGQRIRIDGSRGLVEIIDDQQVPMASSSSGR"
     gene            complement(36805..37254)
                     /locus_tag="PKL17_01525"
     CDS             complement(36805..37254)
                     /locus_tag="PKL17_01525"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNK43432.1"
                     /translation="MNRPPQPAKDRRETERPGPDGLVDRRSGADRRVHERILVDWAVD
                     YRASDTFLFAYISDISAMGIFVRTRTPEPPGARLNLRFSPPGGPAMDLEGEVIWINPP
                     RNDDDESRQPGMGIQFVDLGDAQREQLVRLVRTFAYLPDEDEAIGTC"
     gene            37367..39946
                     /gene="uvrC"
                     /locus_tag="PKL17_01530"
     CDS             37367..39946
                     /gene="uvrC"
                     /locus_tag="PKL17_01530"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNK43433.1"
                     /translation="MSARRRRSEPSSPVAPPDAAPAVPDDGAASAVPAAKEVATAHVE
                     PTSDTTPIPSPEGEPRRRGRRREVPVLTEDDLLVRPSIAELLDKMPTDPGVYLMKDKR
                     GRVIYVGKAKNLRNRVRNYFHRSGDDRPFVKLLDRILSDIETVITSSEKEALLLENTL
                     IKQHKPRFNVRLVDDKNYLVLRLDPKARFPRLEVTRKIREDGARYFGPYHSARACRQT
                     LEVIGRHFRLRTCTDQVMNSRRRPCLEYQIKRCDAPCVLPIPPSQYAEQVRDVALFLE
                     HKSSELLDRLRSRMMTASDELEFETAAALRDQLKAIEITLERQHAVATQFVDQDVVGF
                     YREGDVLEIAVLLIRQGKLVGRRTFRCTGQEFPDEESLSSFVSQYYDRPGIEIPDEVL
                     LPLEIEDAQAKMDWLRERTIELFGRARKVEVLTPKRGPRHRLVDLASKNAAAAYASRR
                     NRTRDTEASLQKLQRRLGLKKLPARIECFDISHLQGSDTVASRVVFLDGEPAKHLYRT
                     FKVRSVENDDFAAMYEVVSRRFRRALGKEPGQTDAGGEVGFSSAQVARRRSAALLPVS
                     IASPAAVVPPAEDAEVPDVDDLEAADAQVEAALGDADAFANDDDEARAADPWALPDLL
                     VIDGGKGQLASALAALKDVGINWAQQLDAIGLAKEREDQSGNRNPDRVFLPRTKDPIR
                     LRDNTAELFVLSRIRDEAHRFAVSFHQKLREKRTIRSQLQEIPGIGPKRQQALLKSLG
                     SVRRIRSASRDELLAVPGMSEAAVQAVLHFFRADRQADGPALPASEIASQIPKSSPPA
                     TTDRQEIRRETAPVATPDVIENAAAAELSALLTDESEDDLERSVAAVIAEEAGGKGRE
                     LGG"
     gene            40281..43283
                     /locus_tag="PKL17_01535"
     CDS             40281..43283
                     /locus_tag="PKL17_01535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNK43434.1"
                     /translation="MMEKSEAAVIVGRAQTYGSGSRTVSKAKQASESKSTKKRAPSKS
                     SKAADARASKPGLQIPRYFSTAGRDPFGEVEWELRNASITGESGKVYFEQKDVEFPKS
                     WSQLATNVVVQKYFRGAMGTPTRERSVRQLIGRVVSTITGWGLKDGYFRTEQDAEVFR
                     SELTHLLIHQKMAFNSPVWFNVGVETEPQCSACFINSVQDTMDSILTLAKTEGMLFKF
                     GSGTGTNFSSIRSSRESLQGGGGASGPVSFMKGFDAFAGVIKSGGKTRRAAKMVILNA
                     DHPDILEYIHCKSSEEKKAWALIDAGYDGSFNGEAYNSIFFQNSNNSVRVTDSFMESV
                     VHDRPWHTRAVRDGSVVDSYRARDIWRQIAEAAHQCGDPGLQFDTTVNSWHPCPNSGR
                     INASNPCSEYMFLDDSACNLASLNLRKFQFPVGHPQAGDFDIESFERAVDLTILAQEI
                     IVDNARYPTDKIGENSHLYRPLGLGYANLGALLMSRGLPYDSDAGRAYAGAITAIMGG
                     RAYRMSAEISRDHGGPFAGYEKNREPFLGVMRKHRQHVDHIDSALCPYDLLQAARAAW
                     DEAIGIGEHHGFRNGQSTVLAPTGTIGFMMDCDTTGIEPDIALVKYKRLVGGGLLKIT
                     NQTVGEALTRLGYDATAQKQITDFIATHDTIEGASGLRPEHLSVFDCAFRAQNGTRSI
                     HPMGHIKMMAAAQPFISGAISKTVNLPSDATIEDIENAYMQAWQLGLKAIAIYRDGCK
                     RSQPLSTSKTEKTQGPAAASGSFLPVQVLIDKLPTEIQQQLRDATSLSLEDFLQHVAS
                     LANPKGQPMAVRRKLPDERRSITHKFAIAGHDGYIHVGMYDDGSPGEIFIRMAKEGST
                     ISGLMDSFATAVSLALQHGVPLKLLVDKFSRTRFEPSGWTGNSEIPRASSIMDYLFRW
                     LEAKFLPEQVQGEQLEMDLPTPAPTAQSVDAPSPTLPNLGSIIAATAARVFRTETDAP
                     TCHECGTIMVRSGACHKCLNCGATSGCS"
     gene            43462..44376
                     /locus_tag="PKL17_01540"
     CDS             43462..44376
                     /locus_tag="PKL17_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNK43435.1"
                     /translation="MSTVTKPWSLDSVVAQLRQARQVPAASRRCCGDKEPLPSRERLV
                     HVVTSLRSALFPAHFGPAELSLDAVDHFVGYTLDRALHTLREQIRRGLMFAGSCGDEA
                     SLRAEKITQQFASELPAIHELLQSDVRAAYAGDPAATSPDEAIFCYPGITAITHHRLA
                     HALHRLGVPLIPRILSEIAHAETGIDIHPGAQIGGSFFIDHGTGVVIGETSVLGERVR
                     IYQGVTLGAKSFPVDEHGNPKKGIPRHPVVEDDVVIYSGATILGRITIGRGSSIGGNV
                     WLTRSVPPNSRITQAQASSEAFVDGSGI"
     gene            complement(44425..45060)
                     /locus_tag="PKL17_01545"
     CDS             complement(44425..45060)
                     /locus_tag="PKL17_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43436.1"
                     /translation="MRNSPFRFIVLGSLLLTVSPTLAKPPSVEPETVSTRAYSAMEGH
                     ARKLGDELGMPAERRDIALGRLRTAFESGKIAAFSKEATVTGIFAYQMGEGGFLVKVK
                     KGQGLAHLSSSEPKVALELKSVTFGAQVGGGSEWGFGVILGLRHAAAFGGEYSGNDRG
                     ATAGGESINITKLTKKGLSASDPSYHELYLVGVSKGLSAGAAIGSLTITRK"
     assembly_gap    45163..45262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(45374..47623)
                     /locus_tag="PKL17_01550"
     CDS             complement(45374..47623)
                     /locus_tag="PKL17_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNK43437.1"
                     /translation="MATESTSSPSPASVANPRPLAFFSAALIAAVLVGSGDVLLAASS
                     SSTPVPGDALAKALLCSIGLVGVPALVMGGLLQWLSSSLAAVFGPQYVRHAMSTLREQ
                     ESEDRKIAALLLALCVVLLVDATVLYGYVKTAALEMANKRNGALSSALVSLATLVLLL
                     PFSVPLYRITHQIVGWLLPRPRVLWLCGLSMLAAVLVIALAILSVDWRIIHFGPWKVL
                     AVWSVALLILATSLARLPRPQLVGMGALLLIIVSFFASVQKFGSEPRSLALIAEETAA
                     GRPLLLLVRRFFDRDHDGYAGRLGGGDCNDHDASVHPGADDEPGNGIDEDCDGADAEK
                     IVEAPAPPEPAQTNTPVIPEPAAKSAAVWNGNWLVITVDTLRADRIHPKTTPTLAKLA
                     AEGFQFGNAYAQAPNTPRSFPSFLTGRLPSDVHFVKQSLNFSPLTGKDPTLFTEFKSA
                     GLHTVGIFSHFYLDRKTGLHLGFDEYKNDAATNLHDSNSDIPEPRITAQVVAKIKAIG
                     KESKPDRKFVLWTHLFGPHSTYMDHPEFPVGKGFKFIKERYDAEVSFTDVHIGKMLAA
                     LAEAGLSEDTAVVVFSDHGEAFGEHKLSGEPLYFHGESLYNEVLKVPLIVYLPKKAGL
                     APRVIADRVRLIDLAPTLLDLSGRKAPASFRGQSLLPLLKGDKLPAPLPAIAEMLPCT
                     AWPKNERVIVDTIDGVEYALYAKFTDNLNELFDLGSDPTQQKNIAAQKPQVVATLRKR
                     LQPFLQKQP"
     gene            47883..48230
                     /locus_tag="PKL17_01555"
     CDS             47883..48230
                     /locus_tag="PKL17_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNK43438.1"
                     /translation="MGTKLYVGNLSYSTTSEQLGEMFHAFGAVRSAQVIEDRMTGRSK
                     GFGFVEMETDAEAQAAINGLNGQVKGGRPLTVNEARPREEGGRGGGGGRPGGGGFGGE
                     RRGGGGRGPRPSY"
     gene            complement(48382..49311)
                     /locus_tag="PKL17_01560"
     CDS             complement(48382..49311)
                     /locus_tag="PKL17_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43439.1"
                     /translation="MTSSARNGKTQGKSMTLWIGIAVAVAVVMFVLVQRSKTRAEADA
                     QRARYEKLRNQKDYTASTFRCTVQFPSGSAGRIKSYDPFTFRGRQAELATCNDATDGE
                     VWAEFYDLPPGDTAEAFIKECFADVTHRLVHGRLAFLFDYAIPSDIDALKDKQQTLSE
                     GSKDGWQVRLSYIPSPHGVGQVNARQVPRSELRPGDIIAELPTFGIPSNTLSPWIWVG
                     IDDFRSQSERDAFRRAERQATEKLKDQQLEFRKAKERFERMHRHFYLMEAAKEVRPHQ
                     VVVLRRFGSILNEKTPQSAAEIIHKMMESVRCE"
     gene            complement(49368..49862)
                     /locus_tag="PKL17_01565"
     CDS             complement(49368..49862)
                     /locus_tag="PKL17_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43440.1"
                     /translation="MSGSPSARLDSPPPLLVDFADDLKQRLVVLVDPSREELRLPFLR
                     SLRPSIDDYAQVFVESAARQAKTFYEAMWQKPFAPSWNPRHSTVRIHVAVPEDFVTNH
                     PRAAAFPRGYFDIASSLSPGVPWACFELLEEGDTLGVSTDGLVGIGERIVWLPRPYLA
                     LGTS"
     gene            complement(49873..53379)
                     /locus_tag="PKL17_01570"
     CDS             complement(49873..53379)
                     /locus_tag="PKL17_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43441.1"
                     /translation="MSEPAQDKRPAQTQTPAPQPFAAPAKTPTPAASATPPPVPTASP
                     VAQNPSPAASPKWSIWNPFAGMFGDTGKPGPGAAPSPAPTATPTPAGPQTGPSPTTTG
                     PGQNQAPATAQTGQNATAPNGQPAAQPAAPKAPDPKEISQLAADIRKETDGHWFASPA
                     STLQILRGRTPEEIAAIKAEYKSHYGTDLDSSLGGSLSGKDKAEYQALISQDSKDPAS
                     KAKAAIAGLQNAEKDNFLGIGGQHADKAAVEKIFKGMDPATRAELQKQGFNVDGYLQK
                     YYSGDELKKVQAQATGDKASEMAADLKMATHSGWFGASTDKNKILDTLASCKTEEERK
                     AVSDAYKQISNGQDLGAMVGDKLKDEDKKAAQALLDGKPDVAAAYQIEAASKPQGMFA
                     FIGLGGPDKERLNKQFEGKSPEECRAIRDAYNATMTPADKKGDANYNALDAKLKGTLK
                     DDDLAKAQQLAESGKVSDELAIKMSMNTGLFGMFTDDKKINSILEGKSKDDIEKLKVK
                     YAELTGKDMEKELTSRTGGDAGFEIQMSLKGKPTTPEDMVAQANERYKHQRGNGIRNF
                     FVDTFGGEAGKDLDRQNQRLNSTYDKAKAGDGKVDQAEFAEINQLADWNKIDAKAVQE
                     RTDSIANAAGTVASVTAGVALTIATAGAGSPILAAALVAGGSGAAMMGTKALVKGGGY
                     GGDELKSDLVDTGFSMAGGMATAGIGQLMQGAKMDAFMRNVLKIDPTKAAGELSAIEK
                     MKMSVLSKGLEGGMGGMASAATNRKNWEGGAGDVFGNMAKDGLLGFGKGAFGGLMGSL
                     PSTLANKDFAKEHPGMMWMQKIPGFGDPAANDKDFSMGESILNGLKGGVGKAAKNYAM
                     DTAMDTLKDGNTWSGGNFLNSLMQHGEDKFNEGKWAKDVAGGVKDGVMSNRLVENKIR
                     SLVGAEQIKEGTKPSDWGSSLVTMEDKYKVGGAKGGISGMFGGLLDGTVDAFTPANEG
                     KQDKGLAIFNSMLTGGMKGVQGGVAGVDAEHRADQAKLEKETLRRMWKNWQGKDAPEG
                     EGELVDKKLALRERLEGLYGPLRPETAATGGTTGQAAPESAATGTGQATEPEYDKATQ
                     KALRRQQQKLEQQKAREERQEQKQAQAQSDAREREINEHHSPLQGPDHRDQIVAQTSQ
                     HDFFPDAPEGRTDQDVFDYLSH"
     gene            complement(53432..54382)
                     /locus_tag="PKL17_01575"
     CDS             complement(53432..54382)
                     /locus_tag="PKL17_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNK43442.1"
                     /translation="MRLSRRKWMARLVTGLALFGCTSKPNQSQNSPVSPALSPEKLRT
                     AAEKRQNFRRGQDARMRSALSPLARVDYQHIPIGQHPVGLEVSSPLHLFASLRSGFGG
                     QLVVSRLGSEVTFSATETVLYNGQPANRATLRKGDVLTLGQTQLLLTGSLDDPGFGIY
                     SDQAEAKRTYSGLHDYPDDEAFVVEAKLFRTAPHPVKVLASRGEPQELISVGDLHFSL
                     PDGEQRRDCTLEAYLETPGGKTLFLIFRDKTSGKPSGSYGAGRFLLADLDPSDTAILD
                     FNQAWNPLCAYSPYFHCPMPSQKNHIPVAITAGEKAYGDH"
     gene            complement(54392..55351)
                     /locus_tag="PKL17_01580"
     CDS             complement(54392..55351)
                     /locus_tag="PKL17_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43443.1"
                     /translation="MQRRVLALCGLLSWTVASAFVPVGPAWAQSAQGPVERTVITKDG
                     SMYRGEVLEYVISSHITLKLSSGESKRIAWDEIDQVSPPKAKAPAAAPSPSPAPPPSS
                     PSPPSSPPPSASLPPSSVPPSSPPPAAAPPVAPSTPLVGSGMERTVTTRDNITYHGEV
                     MEYEVGSHLVLKLGDGNLRRITWNEAKRISPARRKGDTSHVGSPERTVVLRDGKTLRG
                     DLVESVQGMHTTIRLGSGETRRLLWSDIQRITAPLAPGVSSPIPLTGELLVHLDTGSR
                     LLGTYFEYVPEQQLILRHGSGRLYYIPVNSIVKVTITEEGKSE"
     gene            complement(55418..56497)
                     /locus_tag="PKL17_01585"
     CDS             complement(55418..56497)
                     /locus_tag="PKL17_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43444.1"
                     /translation="MHSVRFLQIEPTTRCNFTCGFCCGRYMEQADLPFERFETLLAAH
                     PSVEHVELQGEGESLLHPRFFDMVRALRAKNIKVSLISNGSLMSESTAEELVSLGIEK
                     VSVSMESADPETFRQIRGGKLEKVIAGLSNLMEARRKQNQPKPLVGLSVTVLRDTQDH
                     FSQILSLYKKLGLDGGLTVQPLSTMPLYQKNYPEGLQRQVLSDSEVDDLWVRFRMHRQ
                     LRNIQAAKDKLGIKSFYDELLAGFQPGKRRCPWLTDGLYVNHQGIATACCMVKDTENQ
                     RLGVIGSDGMDSILAARQKLAEELASGVLPKPCAGCELGRFSLMSRWQLVAFALGGVL
                     RTYSPRAVWARLRPSKPKVRLPLLP"
     gene            complement(56583..>57550)
                     /locus_tag="PKL17_01590"
     CDS             complement(56583..>57550)
                     /locus_tag="PKL17_01590"
                     /inference="COORDINATES: protein motif:HMM:NF039450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HNK43445.1"
                     /translation="GLTLNGLTLNGLTLNGLTLNGLTLNSSSTTAPRLASVNDRLVDL
                     GKVSFSNITLGNQTLEDAAIDSATLVARAEDRTYRGSDLVGARLGGTLLNGTSVPLRI
                     DDVKIDADGTVLYSVAVLSDNGPMSLCGTANGVPIPAVVVSGYWDKAASYVDEDDSFT
                     FGCVNAAIGKCVRWGYKPWTTAQECRNNSCKTRKLSDWHRACVRLVRADYCGDGVSHT
                     RNGTIINVYDQIGIQKSSNPGWEVEAEWGADGASCIRHTRWLQANTIYGETDLAYIQR
                     VCPSRLAANNGPRCSLDKTTYNTQFGFNRSSDERPLVRNESPEYQ"
BASE COUNT        10523 a        16926 c        18334 g        11667 t
ORIGIN      
        1 gtggctccga cgcccaaaga ggtgatctgg cgcaaaaaca aagcgcggct ttatcggtat
       61 acgcggtcgg aaaaagtgac gaaaaggacg ccggttctgt gcatcatgcc gctcatcaac
      121 cgggcctata ttttggatct gcgccccggt gcgagcttcg ttgcatacct tttgtcgcag
      181 ggtcacgatg tctttttgct cgactggggc gagtggaacg atgaagaccg cagcctgaac
      241 ttggacctgc ttttgacgca gtacatggcg cgggcggtgc ggatcgtcgc caaagaagca
      301 ggcgggccga tcacgatgct cggctactgc atcggggggg cgctggcgac ctgctttgtc
      361 gcgcagcacc aagagtcgct gataaaaaac ctgattctgc tgacgacgcc gatcgatttt
      421 accgacgcgg gtccgtttgg tgtgtggacg cgacctgacg tgttcccgct ggagcttttg
      481 acatcggtgt ttccagcggt gcccggtggt tatcccgatg ttgggagcaa gcttctccag
      541 ccgttgccgg cggggatggg tcagtttgtc cggctcgaag aaaagctgcg ggagtcgcgc
      601 ttcgatgtcc acggctggca ggcgatgttc cgctgggtca acgaaggggt cgcttttcca
      661 tcgggctgct atcggcagtg gatcggcgac ttctatcagc gcaacaagct ggtccgcggc
      721 gagctggtga taaacgggca gaacgtgctc ttgtcgaaca tccgctgccc gctgctaaac
      781 gtcgtggctt cgactgacat cattgcgcca cgaccgacga caaatgccat cctgagccac
      841 gtcagcagca ccgataagca agaagtcgtc gtcaagggcg gacacgtcgg aatcgtggtg
      901 ggcaaggccg cagcgctgga cctgtggccg aaagtatcgt cttggctggc ggcgcgcgat
      961 tagacgggca gggcaatcgc accgctgccg cttgtgatgg tcagtggttg tgaatgagga
     1021 cttcgcgaac gcctttggcg ccgtccgcgg gtccgacgat gctgtcgcgt tccatgcgct
     1081 caatcatccg ggccgagcgg ttgtagccga tgcgcatttt gcgctggagc atcgagatcg
     1141 aaatctgtcg accctcggac acgatgcgga ccgcctggtc gtatagctcg tcgacaactt
     1201 cttcctcgcc gccttcttgt tcatcgtcgc cacgcggctt gaggatgtcc atgtcgtaga
     1261 tcggcttgcc aagctgcttc agatgctcga cgacgcgatg gacttctccg tcggtgatga
     1321 gtgcgccatg gatgcgtcgc agcgcctgtc cgcggtcggt aaagagcatg tcgcccatgc
     1381 cgagcaggtt ttcggcgccc tgctgatcga ggatggtgcg agagtccacg cggcttgcca
     1441 cctggaaggc gatgcgcgac gggaagttcg ctttgataag gccggtgatg acatcgacag
     1501 acggtcgctg cgtcgccaaa atcaagtgaa tgccggcggc gcgggctttt tgggcgatgc
     1561 gggcgacgca gatttcgact tcttttccgg cgaccatcat caggtcggca aactcgtcga
     1621 tgagcacgac gataaatggc agcttctgga ccggctcgcc gcttgggcca ctgaaatcat
     1681 ccggatcgac accgagcgag agctgtcccg acgctgtgat ctcctggtgt ttttcgactt
     1741 tcttgttaaa gccgccgatg tcgcggacgc ccgccttgct gataaggtca tagcggcgat
     1801 ccatttcctc gacggcccag cgcagcgcga gtgcggcttt tttggggtcg gtgacgacgg
     1861 gcagaagcag gtgcgggatg ccttcgtaaa tcgacagctc gagcatcttg gggtcgatca
     1921 tgatcatctt cacctcgtcg ggcgaagcgt tcatgagcag cgaacagacc atcgtgttga
     1981 ccgacacgct cttgcccgaa cccgtcgtac ccgcgacgag caagtgcggc attttggcca
     2041 agtcgacgga caccggaatg cccgcgatgt ctttgccgag cgccatcgtc agcttgctct
     2101 tggcatggcg gaacgagtcg tcggcgagca gctcttttag gtaaaccgtc tcgcggctgc
     2161 gattgggcac ttcaattccg acggcggctt tgccgggaat cggtgcgacg atgcggaccc
     2221 gcagcgcttc cagcgccatc gccaagtcac tcgacagcga cgtaatgcgc gcaagcttga
     2281 tgcccggcgc cgggacgaat tcatacatgg tgacgacggg accaggatgg atcgctgtga
     2341 cccgtccttt gaccgtgtag tcgccaagcg taccttccag ccgctgcgcc aaatcgagca
     2401 tcgctttgcg atccagctca tgctgcggat gctcggcgaa gtcgaacaga tcaatcggcg
     2461 gtggctcata cgggcgcagc ttgatgtagt tggcttgacc cgcgggcttg cgtggctcgg
     2521 gctttcgggt tgcctgcacg atgcccaagc tggattcatc gtcgggttct gatgggatca
     2581 ccacatcggg cagttccggc gtagccagcg ctgccgccgg ttcatcgtcg ggcagcggca
     2641 tgtgcgaagg cggtccgtcc attttttccg tcgcatcggc gcgctctggc acttgcgcag
     2701 cgatgaccac cgctgggccg tctgccgtcg cgggcgcatc ggttttgcgc gtcggttcct
     2761 cggtgagcag cttggtcggc tcgccatgga tgcccagacc gggcaccgct tcgtcgggtt
     2821 ttggcgcggg ccgccgcgcg acaatcaccg gcgctggttc gtcctcgctc tgctcgggct
     2881 ttttctttgc gggcaggttg gtttgatcgc gatcatcgcc gtaatcacgc acctgccgtg
     2941 gcacacccga gcttggtttt ccagaccccg gcagcgcaat cggcggtccg tcatccagcc
     3001 gttttttggg tagcagcgac agcagttttt ctttgatttg tcgaagctgc ggacgcactg
     3061 cgtcgtgtag tttggctccc agggttcgcg tcgtcagcgg cgtcagcatc gtcaccgaca
     3121 aaagcagccc catcccgagc agcagcaccg ttcccgccgt ccccagcatg gcgatcagcg
     3181 cttccgcggt gtattccccg agcgctccgc cgggtggcat tccagcaatc cggaatcgtc
     3241 caccagccag atgcgccagc gtcatcgtca gaagccacgc cgccgtcccg ccgcccacga
     3301 gctttttggc cagcgtcagt ttttgcgtcc gcagaagctt taccgcaaac acaaacagca
     3361 gcagcgcagg accgatcgtg ccgatgccca gcagcgcata cagggcgctc gcgacggctt
     3421 gtccgacggg gcccatcaac tgcccatcgc caaacatcaa agacagcgtc gccagagaaa
     3481 caaacagggc cacggacagc agggcaatgc caatcgcttc ccgtcgacgg gacgacagca
     3541 ccagtggtgg cggcggttct gctttgccag ctttggcggc tcgacgggca gcgactgctt
     3601 cgcgcacggc tccttcacga gcggtggcgc gggctttggc gggcgctggt tccagcgcgg
     3661 cggcatcctt gtccgcgggc ggtgcgacgg cggcagaatc ggaaaatgtc actttgtcac
     3721 gcggtggcac cgcgatcggc tcgcgcggtg gaatcgcaat cggttcgcgt cttgggattg
     3781 ccactggttc acgcggtaga ttcgccgtcg gcaggctcac gtcttgcggc gggctgggcc
     3841 gtgtgtccag tcgcggcgct tctcgtcggg tttcccgatc gatgactttg gggacgctgg
     3901 cttcgacggg cagccgagcc gccttgcgcg gacggggcgg aatcaccacc tcgcggcgct
     3961 ggcgtgaatc gagaagctct gacggtggcg cggtgaatgc tggcgcccgc accggctgcg
     4021 gatcggcacc cgcttgcact cgacgctgcg cggacaggcg caccggctcg gagtggtgtg
     4081 cttcggaaag atatgactct gcggaaagcg gccccgattc ctcgtcgtca tcgtccaaat
     4141 cgtcgtgtgg aacaaacggt gcgctacgca tgataaaaac cagcccacat gtagtacatc
     4201 aggttaccta gtcatccccg acggtcaact ttcagaccca cgggcagtgg aacactatcg
     4261 cgcatcgtcg agcactcggc aaaaagtgct atcttttcgg ccatgtcgct tgccccgtct
     4321 ccctcgcagt ttcaaaacct gctgctggcg ccgaagcttt tgtccgaccc gcgcaagccg
     4381 attgagtcgc tttccgcccg atttggacgg attttccgaa cccgaacgat gtcggcgagc
     4441 gggctgcggg aaatggtctg gctgctcggg gcaagccaca acgaacgcat cttggctccg
     4501 gcgcaccgcg acgactttac ctggtacgaa ggctacggct ttacgatgga gccgctgcta
     4561 ggtcgcgaca ttctgttttt gctcgacgac cagcccgagc ttggcaaaga gggcgcgcac
     4621 cggcggcgac accggctgct gattccggcg tttcagccgc gcctcgacgg acagtatcta
     4681 ccggcgatgg cgcagatcat ggggcatcat ttgggccggc tgccgctcgg gacggcggtc
     4741 gatctggcag cgacggtcaa gcaggtcaca tttcacattg tcgcgagtct gctgtttggc
     4801 atgcctgccg acgacatttc gctgcttttg cacgacttcg aagacgtcgg attggggctt
     4861 ttttcgctgg tccatctgcg gattccgggg ctgccgtttt atcgcgccga ccgtgcgcga
     4921 cggaaactgg ctcactacgt gcggcaaaaa atccaggcgt ttcgacaaaa gcagatgccg
     4981 gccccgacgt ttttggccca gctactcgac gcggaaggcg aagacggcga gcgtctttcc
     5041 gacgaaacgc tggtgtcgga aatgctggcg tttttgtttg ccggctatga cacgaccgcg
     5101 agcatgctca cgtcgattct ggtagcgctt gctgatcatc ccgaggtgcg gacgcgggtg
     5161 cagcacgagc ttgcgacggt cgatgtggaa cagctccggg cgggcgcgct gcctgagctt
     5221 ccgtatcttg acgcggtgct tttggaagtt gagcgcttgt tcccgccgct gacgtttgcg
     5281 atgcgaggcg tgctgcgcga ctttgagatc gacggctata ccgtgcgacg gggcgacaac
     5341 gtcgcttaca gcgcctattt caccggacgc gaccccgagt tgtttcctga tccgctgcgt
     5401 ttttggcccg atcgtttcgc cgcacagaag cctcggcctt acgcgatggt gggctttggt
     5461 gccggccatc gtccgtgtat cggcaagcgc ttttccctgc tcgagatgcg gctgtttgtg
     5521 gcgcttttgt ttcgccagtt cgatgtgcag tttgcgcccg accagtccac tgcggtgttt
     5581 ttcaatccga cgatgcagcg aagagacggc tactttgcga cactttcgct gcaccgcgcg
     5641 gcggcgtagg ctcatccaac ctgttcggtc atggctttgg cgcttcagaa tccatcgcag
     5701 ggaaatcggg tcgtcgtcat cggcggtggt gttggtggtt tggcgacggc gattcgcttg
     5761 ctcgctgcgg gctactcggt cacggtgctc gagcgggcac agacgcccgg cggcaagatg
     5821 cggcaagtcg aagtcggtgg tttgccattc gacggcggtc cgagtgtcct gacgcttccg
     5881 tgggtgctcg acgagctgtg ccaagtggcg ggggttcggc gctctgacgt gatcgagctt
     5941 gcgccgcttg atccgctctg tcggcacttt tttgcagacg ggacgcagct tgatctttac
     6001 aacgacgatc cgcccgatgg ccgccgatcg accgatgcgt cgtgggcgcg atcgagcgaa
     6061 gaaattcgca aagtgatcgg cgccaaagct gccgaggaat acggacgttt ccgtcgccat
     6121 gccgcgaaaa tctacagcgc cgtcgagcgt ccgtttttgc aaagtccgat accttccaat
     6181 ccgctcggac tggtgttcca caaccgaatc agcgatgtga tggggctgtg gaaaattgat
     6241 gcgcgacgga cgctgtggca ggcgctgtgc agcttttttt ccgacgagcg actgcggatt
     6301 ctgtttgcgc gctacgcgac gtattctggg gccgatccgt ttttggcacc ggggacgctg
     6361 gcggtgattc cacacgtcga gcaaggcttc ggcgtgttcg cggtaaaagg cggtatgtat
     6421 cgcgtggcga cggcgcttgc ggagcttgtg ctgcggcttg ggggttgcat tcgctacggg
     6481 gcagatgtcg agcggctgat tctcgacgac aaaggcaagc aagtgaccgc ggtccaggtc
     6541 gcaggtgaac gcatgtttgc cgacgtgttt gtcgccaact gcgatgtgcc gcagctcact
     6601 ttacggatgc tcgacgggac caaggtcggc gcacagctat cgcgccttta tgacaagctt
     6661 cctccgtcgc tgtcggctgt gctgcatctg gccgtcgccg atcaggctgc gcagcttccg
     6721 ctttgtcatc acaacgtgtt tttcacccgt gactaccggc aagagtttgt cgaacttgtg
     6781 gacggtcccc cgacggaccc aacggtctat ctgtgcaatc ccgattttgc ccaggatcgg
     6841 cagcgctggt tttttctgac caattcaccg gccctgccgc gcgacgggga gccgagcaaa
     6901 cagtcgccgt ggacagagcc gcttgtgcag acgtgtcgac agcgcgtcgt cgaaaaactg
     6961 gcgagacacg gcatttcata cgaccggcat gtacgcgccg aaggttcggt gacaccgcaa
     7021 gattttgcgc ggcttttccc agcgagtcgc ggcgcaattt acggagccgc ggcatcttcc
     7081 aaaattgcag cattccgtcg accacccaat gaggtaaaag gccttgcgaa cctgttttgt
     7141 gtcggtggtt cgacccatcc cggcgctggc gtaccgatgg tgatgctgtc tgcccaaatc
     7201 gtcgctggcc tgtgtcagaa gcgctttccg cgcccgcgct gacggtgcgg actaggcaaa
     7261 atccaccggc gcgctaagat accggctccc caaacgaaca acacactgcc gagccaatgc
     7321 tcggtaacag actttttcga ggagaaaatc gccatggctc agcttgttca ctgtcgcaag
     7381 ctcgggaaag agctacccgg acttgacttc gttcccttcg acgatgcact cggcgagcgc
     7441 ctccagcgtg aagtgtcggc tcaggcctgg cagatgtggc tggagcactc gaagatgctg
     7501 atcaacgagt accggctcga tctcatcacc cagcaggcgt tcgattttct tcaccgtcgc
     7561 tgcgaagagt ttttctttgg cgaaggctcc gagctgccga aggagttcgt cgccccggaa
     7621 cacaaacact agtcactcac gctggccacg atgggtttcc gaacttccgt ccaggttcgc
     7681 ttcggcgacg tagatcacgc gggaatcgtc tattacccgc actttttcat ctatttccac
     7741 gaagcgttcg aggacttttt caacgacgcg ggcataagct atgtccagct tctcgacgag
     7801 cggcggattg gttttcccac tgttcacgtc gaaacggact acaaagcgcc gctgcgttac
     7861 ggcgaccgcc ttgatatcga agtgtccgtc gaaaagttgt caaaccgctc gatggtgatg
     7921 tgttacgaag gctatcgtca tcgcgacgga aaacactcgg tgacatgccg catcacgacg
     7981 gtctgcgtcg acatgaacac tttttcctcg cgtgagattc cccaggatct gcgtgagcta
     8041 ttcgagcgct tccgccgatg agtggcccac gcaaagccgg aatggttgcg gtgctccgtc
     8101 cgcagcttgc cgaagacgga gcggactttg cgctgctgtt ggttcgtcgc agccagcggg
     8161 ccagttttat ggccaacgcc tatgtttttc ccggcggacg ggtcgatgct gccgacgcag
     8221 agcatgatcc cgaatggccc agtcgctacg ctgccgcgcg tgagcttttt gaagaagcgg
     8281 gtctgcaagc ggcgacgccc aagtcattgg tccgttttgc ccagtggatt actccgtcgg
     8341 cagagccgaa acgctttgat gccgactgct atcttttgtc gatcgatgcg acgacggctc
     8401 ggcacggcga tgccgttcaa gtcgacgggc acgaggtctt cgatccgctg tggctgaccc
     8461 cgcgggaagc gctggctcgc tatcaggccg gtgatctcaa cttgccgcca ccgacggtct
     8521 gtacgatcga agaactcgaa gcggagcggc tttctgcgct ttcgattttg cgccagcggg
     8581 gtgagctgcc gacggatgca acgctgcaaa ccgcatcgca gctggtcttt gcgctgattg
     8641 cgtcgtgcgc agcgtcgcga aaaccctatc cgctgctgcc caaactgatt gccgacccct
     8701 cggggactgg gatcgcgatt gtcatgccgt gggattctga ttttgccgcg cagccgggcg
     8761 acggaacttc ctttgcggcg atggctgaaa atgggccgcc agtgccgcga cggatttcgc
     8821 gctgcattct gcacatggcc aaagccgacg agacctatgc ggtgcagcct ggccggaccg
     8881 aatccgtgcg ctggcagatc gagcggctgc ccggttagtc cgcgatgcgc atcctgctgc
     8941 tcaaccagta ctaccatccc gacattgcgg cgacggcgca gctttgtacg gactgggccg
     9001 agtcgctggc atcgcaggga catgatgtga cggtgctgtg tggcagtggt cggtatcgca
     9061 cgccgcacct ggggcttgtg ccccagcaga cggacgactt gccggcagaa cagacgcggc
     9121 gcggcgttca cattgtccgc gttcccgtcg aagatgcacc gccaccgatt ggtgcggcac
     9181 cgtccctgcg tgagcggttc gacaagctgt ttgggcggct ctctcgctat gggcagtttg
     9241 tttcgcaagc gacgctgcgg ctgcgccgtg tgccgcgacc cgatgtggtg gttgcgctga
     9301 cgacacctcc gctcgtcggc gggcttgggc ttttggcgaa aaagctccac ggagccaagc
     9361 tggttttgtg ggtccaagat gtctatcccg acttgctgcc agcgatgggg cttatggatg
     9421 cgggatcggc accgtatctg tgcctggggc agtttgcgcg gcttttgtat cgcagcgcgg
     9481 atcgcatcat tgcgctcgat gaagcgatgg ccgagcggct cacaaacgcc ggggcggatt
     9541 cgtcgcagct gacggtgatc gatcactttg ctgatgccca ggaaatccgt ccgcagccgc
     9601 gtgggccaag caagattcgt cagcttgtcg ggctttccga cgaatttgtc gtctgctacg
     9661 ccggcaacca cgggcgaggc cacgactttg acaccatttt acaggcgcta caggcgcagg
     9721 cggacagccc acctggcatg ccgccgattc actggctgtt tgtcggtgat ggcgatgaaa
     9781 aagccggtct tgtggcgcga ctgccgacgg cgatgcgtcc gtatgtccac tttttgccac
     9841 cgcaagcgcg gcacgagctg cccgatgtgc tggctgccgg cgacgtcgga cttgttgcgg
     9901 tcaaagcggc gatgcagggg cttttggcac cgagcaagct ctatgggctg ctggcagcgg
     9961 ggcgtgtgat tgcctacatc gggccgcagg aaggccggat tccgtcgctg ctgcgtgaat
    10021 gcgagatcgg aatttcggtc cgcaacggtc aagggccaga acttctgcag gcgctttggg
    10081 cgctggctgc ggattcggtg cgctggaagc ggatggcaaa gctggcgcga cagcttgccg
    10141 aaacccgctt tgacaaaccc atcgcgatgg cacagcacga gcggcttctt ttcgacgtgg
    10201 tgggatcgcg ctgatgcggg cggttggttt ggggctgctt ttgtcgctgc tgctggcgct
    10261 cttgctgacg ccgcttttgc gacgggctgc ggtttggctc aaagcgctag acccggtttc
    10321 agcgcgcaag ctgctgcgtc cgtcgcaagt gccgcggctc ggcggggttg ccatcgcgct
    10381 gtcgttttat ctgaccgtgg ttgtgctgtg gtcgacggga agcgtcgtgg cgcgagccac
    10441 cttgcagcaa ggcacgcacg tcttgccgat tttgctcggc gggattccga ttttgctgct
    10501 tggaatcgtc gacgatctgc gtggcatgcg agcgctgcca aaacttctcg tcgaaatcgc
    10561 ggtggcgctc gggctttact ggcagggact gcgggtgctc ggcacgagcg gtccgtcggg
    10621 gccgattgaa ctttcgcagc ttttgtccgc tgttgtgacg gtggcttggg tggtgggcgt
    10681 cgtcaatgcg ctcaacctga ttgatgggct cgatgggctg gcgtccggtg tggcggtgat
    10741 tgcgctcggc acaacgacgg tggcggcgct gcttcgcggt gagcttttgc tcgcgtttct
    10801 gaccggctct ttggcggggg cgacgctcgg ttttctacgc tacaacttca atcccgcgtc
    10861 gatcttcatg ggcgattccg gcagcttgtt tttgggctat ctgctggcca cgtcgtcgat
    10921 ctggtcggtg cgcaagtcag cgacggcggt gctggtggtg tttccggttg tggcgctggg
    10981 actgccgctg ctcgataccg ggctgacgat ttcgcgtcgt tttttggccg gtcgtccggt
    11041 gatgcaagcc gatcgcgatc acgttcacca tcggctgctg ctcaaaggtc tgccggtgcg
    11101 acgcgcggtg ctgttgctgt acggtgtctg cttggttttt tcggggctgt cgctgctgat
    11161 ggtactgggc agtccgtggg cggcgcggct cgggctggcg ctgtcggtgg gttttgcgct
    11221 ttcgcttggt tactggtttg gctatttgcg atccggtccg cagggtctgt gggcggcgct
    11281 cacccgacgc gggcgcaccc agaagctgtt ggctcgattg gatgtgctcc aggcggagct
    11341 gcaagcggtg cagtcgcttg tggcgctcga acaagtcgtg gcggagttcg gtgatttgct
    11401 cggggtcacg ctgcggctcg accggcaaac cgatgcagtc gaaccgggga gctatccgat
    11461 catgtcgtcg ggaaaacagg ggaaattgct cgcagtgctg cgggtgagtc cgccagagca
    11521 tgaaagcaat cccgacgagc gaacgctgat cggcctgttc tgcgatgcac ttgcaccgac
    11581 gctgctgcgg gttttatcaa ccgaatcgta gctacgcgcg ggatttgaca agcagcgact
    11641 ggcccgtcat ttcaggtgga acaggcagtc ccatgatgtc gagcaccgtc ggcgccacat
    11701 cggcgaggcc accttcgcgc agccgctgcc cgcgcaggga atcgtcgacg acaatgaacg
    11761 gcaccggatt ggtggtgtgg gcggtgtgcg gctgtccggt gtccggatcg accatttgct
    11821 cgcagttgcc gtggtcggcg gtgataagga cggcagcgcc agactttttg gccgcgtcgc
    11881 agattcgacc caggcaagca tcaatggtct cgacggcaga taccgccgca ggtagcacgc
    11941 cggtgtggcc caccatgtcg gggtttgcga agtttatcag gatgaagccg tactgcccgc
    12001 tctcgatcgc ggtgactgcg ccgtcggtga catctgcggc actcatggct ggtgccaggt
    12061 cataagttgc gatgtctcgt ctggacggca cgagtcgacg gtcttcgtgg gggtagggcg
    12121 cttcaattcc gccgttaaag aaaaacgtga cgtgcgcgta tttttccgtc tccgccgtgc
    12181 ggaactgccg aaaatcgcgc gccgacagat actcgcccag gttgttgtgc agcgactgcg
    12241 gtgcaaacgc cacaggcaga cccagttttt ggtcgtactg ggtcatgcag acatagcgcg
    12301 cgagccttgg gggcctgccg cgatcgaatg cgtcgaactt ttcttcggtc agggcgcggc
    12361 tgatttcacg ggcgcgatcg gcgcggaaat tccagaacac caccgcatcg ccgtcttgga
    12421 tggtggcgac ggctcgtccg ttttcggtga gcacgatcgg ctggatgaat tcgtcggttt
    12481 caccgcgcgc atacgcggcc aagatggctt ctgctgcacc cgcgctggcc ggttcgggac
    12541 gaccttcgcc gcgaaccagc agacggtagc cacgcgcgac ccgttcccag cgattgtcgc
    12601 gatccatcga atagaaacga ccagcgagcg aagcgacacg accgacaccg atcgcagcga
    12661 gcttggcttc cacgtcgcgc aagaaaccgg ctgccgactg cggtggcgtg tctcgtccgt
    12721 caaggaacgc atgccaagcg acctttgaaa cgccgcggcg cttggccagt tcacacagcg
    12781 cgtagccgtg ttcgagcgtg gaatgaatgc caccgggcga attgaggcca agcaggtgca
    12841 gggtgccgcc gctggctttg acggcatcga ccgcggcgac tagctcggga ttttcaaaga
    12901 aatcaccgtc ggcaatcgct ttgctgatcc gcaccaagtc ttggtagaca atgcgaccgc
    12961 cgcccaagat ggtgtgaccg acttctgagt tgcccatttg gccttcgggc aagccgaccg
    13021 aaaggcccga gcagtgaagc agcgtcttgg gatactgcgc aagcagccgc ggccacgtcg
    13081 atttgtgcgc cagctcgacg gcattcgcag ccggttcttc gcgatggccg aggccatcga
    13141 ggacaatcag cacaacgggt ttcatgacgg gaatgctacc gaaagatgat ggccgctgac
    13201 gagggccttg cgtgctagcg acggatgccg agaagctgat ccatcgtccg ctgatcggca
    13261 tcggggaatt ggtactgacc aaattccgac gaagcaaccc agcggaagtc acgcacgcgc
    13321 ttggcgcgga tcggacgacc gggaatcagc ttggcggcat agagcgccaa gatgacttcg
    13381 taaccgtcgt agatgtggtg tttttcaccg atctttttgc cgatctcaaa gtcggcatca
    13441 agacgttcgc tcagctcgcg gcggagcgca tcttcatcgt tttcgccagc ctcgaccttg
    13501 ccgccgggga attcccactg cagaggtagc accgccgaat cgcgacgctg ggtaatgagg
    13561 taccgtccat ctcgctcgag gacggctgcg acaacgcgga tgaccttttt catggaccga
    13621 acgactcctc gcttaagcgg cattccaatc taccagagat ttcagtgggt tggggatgct
    13681 ctgcggcagc tggcgggctt ttcgcagcac ttgagagcgt atcacgcccg cgcagcttgc
    13741 ggcatgctca ctggactctt tgtcggctgc tccttgcgca gcctgtaagg ggtacctatg
    13801 atcggaactc actaggagga atcgatgggt ctaatggatt tcgtcaaggg tggcgttcgt
    13861 gaactggcga ttgcccgccc ggacagtgca aagggaatgc tcgtctacaa gcatcccgat
    13921 cccaccatcc ccaacaaagc gcagcttacg gtcggtaccg acgaagtcgc gctgttcttt
    13981 aaggacggtc agtttgtggg acagatcggc gccggtcgtc acacgctgga atcgtccaac
    14041 atcatgtttc taaaccagct cgtcgataag ttcacgggcg gcaacgtctt caaagccgaa
    14101 gtttggttta tcaccatccg cgaggtcgcc ggcttcaagt tcggcggtcg cattggcgat
    14161 gtcgaagacc ccaagagcgg tcttgccatc ggcgtcatgg tccacggcga atactcgctg
    14221 caagcgaccg atcccaccca ggtcatttcg tttttcggac agcgctcgtg ggccaatgac
    14281 gaagagttcg tcggctggtt ccggcagctt ctgctcaaga cgatccgcga tcgcattgcc
    14341 aagctgctcg tcgccaaaca gctgccgctg ctcaacatca cgtcgggcgc gctcaccgaa
    14401 gaaatcgaag ccgaagtgat cgaaggcctg aagccgcaca tgcagggcta cggcatgcgg
    14461 gtggtgcgcc tgggcaactt cgtcgtctcg atcaaggaag aagacgagca gacgctcaag
    14521 tcgatgtaca aggacgcggc gcagattcgc atggccggcg ggctcggtgg ctaccagcag
    14581 ttcgcggctg gcaaagcgat catgggcgcg ggcgaaggca tggcgcgtgg tggtgacgga
    14641 ggtggcggcg gtggtggcat gctggcaggc gcgggtctgg gcgtcggcat ggcgatggcc
    14701 aacatgtttg cgcagtcgac ccgtccggca gcggaagccc cggctccggc ccaggctgcc
    14761 cccgcagcgc ccgcgcagcc gagccttcag gaccggctca agaagctgaa gatgctcaag
    14821 gaagaaggcc tgatcgacga tcaggaatac gccgccaagc gcgccgagat actcaaagac
    14881 atctaaccca cgggcctggg gctcggtttt gtgagccgag cccgccgtgg gctgacctgc
    14941 gccgcgctag tttttgggga aaacgcaggg cttgccggcg caggtgtcgg tgacgacgcc
    15001 gccgtctttg aggaaggctt gcagctgcgc tttggccgct tccgtccggc ggatggcttc
    15061 gtgcaccggg ttgtctttgg gcggcacgtt gtcatcgccg gggcgcggct tttgcccgac
    15121 atcgaactgg acgtaagccg atggcacagg gccgctcgct tgtggaatgc cccacaccgt
    15181 ttcgaccggc tggttgagca gcggaattcc catcgtgcgc accaccatcc gtgtcgccag
    15241 attcggcact tgtccgtctc caaggccttc ttggtagagc acttgtttgg ccgatcccgt
    15301 gcctggcaag ggcgctttga cgacatacgg cgcgtaagtt gccggatcgg tgtagtccca
    15361 aagctgctgg cttagcgcaa tcagcaactg ccgatcgagc gcatcgtgat aattcgacga
    15421 cagcagcggc accaggtctt ggaaatcgcc gctccgccac atcatcaggc tccagaaacc
    15481 gccggggacg ttcaacgcgc cgcgctttac gtcgggcgac agcgccaaaa acgtcagacc
    15541 ctggatgcca ccgttcgaga tgccgtaata aaacacctgg cttgaatcga cgatgacttt
    15601 gccgtttacc cgcagctctg ggatttgccc gagcgacccg tctttgacaa gccgcgcgag
    15661 caccgcaaag tttacgtgcg cctgctggag ccggtcggtg atctgcacga agttgttaaa
    15721 gttcgacatc acctgaccag cgacgtagag cccgtccgct tccgtgagac cgatccagtc
    15781 ggtgccgact tgcaccatgc acagccgatt gacgatctcg cgctggtatc ccgagtccat
    15841 ttcgccgagc gcgccaccaa acagcccgtg gccataaatc atcaccggca atgggcccgt
    15901 cgcggtctgg gcgcacttgg gaatgtgcac caccagcggg aaccgcccat agccgcgtgc
    15961 tttcggcgtc ccactttcgt cttttagcag ccgtccgttt tggtcgtcgc tcaaaaatga
    16021 tgtcgcgcgg aagctgccaa tcacctgccg aagcagcgga tcgcgtggcg cgttttccac
    16081 tttgtcgatg gtgaaaagcg gcgaaggcgg ttccggcgga tcggtcggcg tcagcttggc
    16141 cagcgcttga tcgcgcatct tcacaagctg tccggtgacc tggctgtcgt cacccgtcgt
    16201 gaaatcccac gcaagctgca ggcttttttt cgcgatgccc tgtttttcca aaaacggaaa
    16261 cagctctttg tacgtctcgg ccagccgcgc acgaatgctg ccgggttcga catcgccatt
    16321 gcgaatcgcg gcaaaacccg aaagtggcgt cagcggctgt ccatcgctgc cgcgcagacc
    16381 cgacagcgcg acgacgtaac gggccttggg ccgcagccgc acttggggat ggatcaaaag
    16441 cgcctgccgg tccccatccg tcgcattgcg gtctagctcg gcaaacaggg ggattcgctg
    16501 cccggtctcg aaatcgaaga gctggaccgg cgacgacacc ttgatcgagt cttcctcgtg
    16561 tcgaggcgcc ggcaggttgc ttccatcgat cgtcgccggg aaatacgcga caagctgcac
    16621 cgccggacta aaaccatcgc gccccaaaag cagcgccgga tcgagcgcga cgcctttggc
    16681 tgacgccggc agcacgccgg gcgggaagct gatccgcatc acgcctttgt catcgggact
    16741 gcggtagtag ttcgacggaa acggcgtcac gcagtcttct ttttcgccgc cccccagcgg
    16801 attgcagcca gccgcgagcg gcggttccgg cagtggcgtc aaatcgacgt ggactgtgca
    16861 cgaagccaag agcggtgcgg ccagcaggaa cgaaccaagc agcggaaaag cgagcgtccg
    16921 ggtgcgcatg cgcggcagca tatgccagaa ccggcgctgc gcagatcggc caaattgcgt
    16981 cgttagcttg gggtgacgcc ttgcaggatg gccagggact gctgtttgcg tgtggcttcg
    17041 ctgtgcagcg catcgtcact gatgacgtga tagcggtgca gctttgctag gtagtcgagc
    17101 cgcagatccg acgaaggccg cacgaacact gcatggatgc gggcgcggcg ggcgacttcg
    17161 gccacaagct cgctttgaat gtggcattcg ccgtgggtga caagaaacag cgtgacgcgc
    17221 gctttgtttc cggcagtcat cgtcgacagt tcgcggacta ggtcggaaaa tacccgcttg
    17281 tagttgcggc cccggttcga tcgaaaaccg agcaggtacg gcaaaatccg tcgcggcgat
    17341 cggtcggccc gcagcgcatc atgaagtcgt ccgtcgaaaa aacgcacttg cacgggctgt
    17401 ttgctgcgca aaagacgctg gaccagcgac agcgcaaagc cgcgggcaaa cacctggcgc
    17461 agacctcgca tggatggcga gcagtcgacc agcacgtagc tgagcgggtg cggtgcttcg
    17521 acttggcgtt cgcgacccag gtacagaagt tcgctcagcg caaagcgggt ataaaacagg
    17581 tcgtcgtcgc tggccagctc gcttggcagc agtgcatcga gcccgccgcg gtgaagcagt
    17641 cccgcataac cccccgtcgg atagctctgg gcaccgctga catgctgggc ttccagtacc
    17701 gatggcaata ggtcgagcga aaagcgcacc gtatcgcgca gatcatcggc atcaaacagc
    17761 gactccagtt ccagcaggtc gacccggtcg tccgatgccc gcgacagaag ctgggtcgat
    17821 ccttccgaca ggtgcaaaag ccgcacggct tgtgcatcaa gaagctcgca gcgcagcaga
    17881 agcggcaccg acttcgcgac caggtccgta cagacccgcg caagccgcgc aagttcgtgg
    17941 gtctgttccg ccgggctcgg cagtgcgctc tcggcaatcg ctttggggga aagctcgatc
    18001 gtcgcaagct ccatcgaatg cagatccggc acaaggggcc gcagaagttt cgcgagcagc
    18061 accgcacaga cctcgtcaga cggcctgttt gcggcaatgt cgaccaccga gggcaccgcc
    18121 aaaagcgtct tgagcagcgt gcgatagcgt tcggtggttt cgcgcaaagc gggcggtggc
    18181 ggcacatcga gacccacgcg aagctggctc gctggcgtcg acaaaagcag tccaaagtca
    18241 tgcacaagcc caagcggcag tggcacgccc agtcggcaca gcaggttgtg ccaggtgatg
    18301 gccggcgtca gactttcgtc tccgcgatgg agctgcgaca gccgaagcga cagcttttgc
    18361 cggaccacaa atgcgcgcag ggacatcgtg agctttcacc ttacccgcaa atggcaatgc
    18421 cgtctgcgtg atatacggga tagatggcgc tgcgaaagat tgctcagatt ggacatccgg
    18481 tgctgcgggt tttggctcgg cccctgtcgc gcgaagaact tctgtcgcag gcaggtcagt
    18541 cgctcatcga cgatttgatt gcgacgatgc gcgatgccga tggggcgggg ctcgcggcac
    18601 cgcagatgta cgaaagcgtg cgggtctgcg tcatcgaagt cggccaaaac gcgcgctatc
    18661 cgtacaaacc gaaaattccg ctgaccgtgc tggtcaaccc ggtgctgacg ccgctcgatg
    18721 cgcggacgtt ttccaactac gaaggctgcc tgtcagtgcc caacctgcgc gggcgcgtcg
    18781 acaggttttg cgaagtccgc gtgcaagcct gggatcggtt tggcaacgaa ctcgactttg
    18841 tcgcgcgcgg catttcggcg ggcacctacc agcatgaagt cgaccacttg gatgcgacga
    18901 tttttgtcga tcgggtcaaa gacacccgct ctctgtgcac ctggcaagag ttcgagcgcc
    18961 accaccgcga agccttcatc aaagaagtcc aggccatcgt cgccgagttc ggttcgtagc
    19021 ttcagaagct ggaagtcgta cacagatcag cgtgtgcaac gatccagcgc tgtgaagcgg
    19081 tgaaaaaacc gtgcggtgca gcggttacgg gatgggcgtg acttccaccg gaacgccgct
    19141 gagcgccgcg gtgccggaca aaggatcaat caggtggtcg tcggttagct cgtttgcgtt
    19201 ttggccggga agtgcgcctg cgacacggag cgtggcggcg gcttgctgat ggccaaagcc
    19261 gtgcggcaag gatacgacac cgggcatgac ttcatcgctg accgaaaggg tgacttcgac
    19321 ctgaccgacg cggcttgtga ccttcacttt gtgtccatcg cggagcgaca gccgctctgc
    19381 gtccgccgga tgcacaatca ggtgtgatcg gtccgggcct ttcaccaggg acggacagtt
    19441 gtgcatccac gaattgttgg tgcgaagctg ccgccggcca atcagcaaaa gtccctgctt
    19501 gggcgcctgc agaagccagg tcttcacccg cgtgacatca tcgaccagga tctgtggtgc
    19561 caggtcgatt ttgccatccc gtgtccggat gcgatccgaa agacacggcg acagcgcgcc
    19621 caggtcgatt ccgtgcggcg acgacttgag ctgcgcaagg gaaagccctg gccgcccagg
    19681 gacaaagcca ttgccatatc gtccgctgcg cagtgccaga tcgacgatgt gttctggggt
    19741 tgggcgcagc cccaggcgat gcgcaagctg gagcgcgcga tccaccactt tgaacccggt
    19801 tcgcacgccg cccagccgca tcgacaggtc gtacagaatg tcccagtcgg tgcgggtgtt
    19861 ttcagacggc tcgatgaccg gcagcgagta tttggcggtg ttgcgtacct gcaacgcatg
    19921 gaacaccacg tcgtactgcg accgttccag cgcgtgacga cccggcaaga tgaagtgggc
    19981 gtgccgcgac gtttcgttta tgtagtagtc gagcgacacc atgaattcca agctggcgag
    20041 ggcgcgatcg agccgggcgc tgcttgggac cgacatcacg gggttgccca ggacacagac
    20101 cagcgcgcga atctgacctg ggcctttggt ttcgatttct tcggccagga cggctgcagg
    20161 caggttgcca ccgacttcgg gcagtccgcg cacgcgcgat ttccagcgac cgtgatggtt
    20221 ttcgaccacc ttgcgtccca gcgcgcccag gtcaaacgcc ggacggggga acatcgcgcc
    20281 gccttcgcgg tcgaggttgc cggtacacag gttgagcacg tcaatcagcc agtttgcgac
    20341 cgtgccaagc tcttgctgac aggtgccgat tcgaccgtag gcgacggcgt ggcgcgcggc
    20401 ggcaaagtcc cgcgccagcc tggcaatggt gtcctgcggg atgccgacgg cggcttcgac
    20461 agaagcgggg gggaagtcgc ggaccagtcg ctcgatctgc tcgatgccgt gggcgcgctt
    20521 gcgcacgtct gcaaggtgta cgcgctgctc ggcaaacagg acgtgcagca tggcaaggag
    20581 gagcgccgcg tcgccccccg gacggatgaa gtggtgttcg ctcgcccatt cgctggtttc
    20641 gctgcgccgc gggtcgataa ggaccagctt gccgccgcgc tcgcggatgc ctcggatgcg
    20701 gtgtttgaca tcgccgagcg acatcaagct gccgcctgaa gctgccggat tggcacccag
    20761 catcaacata aaatcggtgc ggtcgacatc gggaactgga atcaccgtct gctcgccgta
    20821 caaaagcagg ctggtcagaa gccgcggatt tgcatcctgg gagtttgcgt caaagcgatt
    20881 tttggtcccc agcgcgcgca gaaacccggc atacccaagc agcgcgtgca ggttgtgcac
    20941 ggtcgggttt cccgcataga ccgccaccgc gtttcggcca tcgcgacggc gaatcgaatc
    21001 cagccggtcg gcaatttccg ccagcgcttc gtcccaaccc acggggacaa agccgctagg
    21061 cgtgcggcgc agcggctgcc ggatgcggtc gggatcttcg taggtttcgc gcagcgcagc
    21121 ggctttggga cagatgtggc cgcgtgaaaa accgtcgtca cgatcgccgc gaatgtcgct
    21181 tacgcgaccg ccttcgactg tgacggagag tccgcacatc gcttcgcaga ggttgcaggt
    21241 cgtaaaatgg gtgcttctgc tttgcatcgg gcttcctttg gcgggttatc caacgggcga
    21301 ctgactgacc acgctatcga gcagggaatc aatttcggac acggcgcgca ggctctcttc
    21361 gctgccatcg ctttgcagcg cagcccgcag ctcgcccagg ctccgcagct tggcaaatag
    21421 ctgcacatcg gacagctcgc ccgcccgctg gagtccaagc cgcaaggtct ggagttcatc
    21481 gagcatcgcc cggcgtcctt gttcatcgcg cggaccggcc ttgtgctgac gatggcggcg
    21541 cagcaccgga tcgaccagac ccgccagcag atcggcctgc tcgacggtgt tccagatgta
    21601 ttttagaaca aacaggtcgc ttacgtcggc ttcggttcgt ccatcgcaca gcgccgacgc
    21661 ggcacacagc ttcaacattt tgatgagccg gcggtcggac agaaagattc cttcgctgcg
    21721 aatctgcgcc gtcagtgctt tgtactcgct caaaaattcg tcgctgaact ggagccgacc
    21781 ggccagctcg cgacgaagct gcaagatgtc tttggccgac aaaagcggcg gaccgcttgg
    21841 cccacttggc gcggtgctgc gtcccccgag cagtctcttt tcgtgttcaa gaccgatgcg
    21901 cagtagctgc tggaagtgaa acccttcgag gttctgcgag cgcacccgca aaaggaatcg
    21961 atcgagcagc gccgccaagc tgtcatccga tggcacttcg ttgctggcgg caaacagtgc
    22021 gagcagcggc acatcgacgc gctcggcacc aatttgcacc cggcgttcgt tcaaaagccc
    22081 gagcaagctg ttcaaaatcg ccgagctggc cttaaaaatc tcgtcgagaa acgcgatctc
    22141 tgcttccggc agcatctgtt cggtgcggcg gcgaaagctg ccttcgcgaa acgcttgaat
    22201 gtcgaccggg cccaaaagtt cgttcggttc ggaaaaccgc gtcagcaggt attcgaaata
    22261 gcgggcgtcg agactctgcg ccaaggtgcg aacgagcgcg ctcttggccg tgcccggcgg
    22321 accgacgatg agcatgtgtt cacccgcgac caccgacaga agcaaaaggc ggatgatttc
    22381 atctttggcg agaaactctc gggtcagccg tcgccccagc tcttggagca gcacagcgcg
    22441 atttcttgca ccagaatctg tcgtcatcgg ttgtccatcg tatgggggtt cgtcgtcagt
    22501 gtgcggggcc cgcagcgctt tttgcacagc tctttttgtc gctgcaagct ttcgggccgg
    22561 gcgacttcct ggcacaagcg gcggtggcgc tgcacttttt cgtagatcgc gccacttgtc
    22621 ccgatgtccg ggggaaaaac cagcgccggg tccacgctgg cgagcgccgc aaacagggac
    22681 agggattcgc gcagcggctg ctgatccgtg accgcaagtg gtggaccatc tgccgtcgcg
    22741 acccgcaggt gaaacagcgc aaacagaccg atcagcgtgc gcagggaacc ttgatctagc
    22801 tcgtcggggt tcgcttccag cgccgcttcg acgctgcgac caatgcggga cagcgcttgg
    22861 gtgctgccgt gccgaagctg cacagtgaca aacagccggg caatccgtgc cacttgcagc
    22921 cacgggacaa gctcgggacc aagcagcggg gccggcggcg gacgcagaag ctgggtcagc
    22981 ggactgccga gtcccaggta gtgccacagc gtcttttccg ctttgtcgat gggcagcgcc
    23041 agcacgtcat gttcaatcga cgcctggggc tgcggcagcg gctcgcccga cttgggtcgc
    23101 agcgcccaca gaccagacaa aagactgtag cgcgccgtga gcttgggcac aaagtcggcg
    23161 gcttgcgcgg tttccaggct tggctcgggc agcgactccg ccgcggcgat gagctggagt
    23221 cgaacctgct ggatcagacc gtctctgcgg ctttgcgaca gcgtgcgtcc gcgtggatgg
    23281 aacagaaaca gcgcatcgtg gatcgccgac agaagcagaa gcgagtgcgg gtcgtcggcc
    23341 aagggccttg gctccgtcac aatgccgatt tcaccaagct gctgcttttg cgctttgtgc
    23401 agagactgtc cgacggctgt ttggtgaagt tcgggggcag cgcggagcag tcggtcggcg
    23461 cctttttgca aaagtggcat tccgacggac accaccccac ccgacgcgag cggataggca
    23521 aagcgggcca gaaaagctgc cgcaaagtcg cggtctttca cccgcgctcc ggatcgctgg
    23581 ccaaacccca cagcggcagc gtcgaaaaaa aggaaatcgg ttcgcagtgc aggaccggaa
    23641 acgaatcgcg cagggccgcc ggttcgatca gcgcatcggg cagcttggtt gcgagcgact
    23701 gcaagagccg cagcgaaagc ggactggcca gtgacttggg caccgcaatc ggctgggttt
    23761 ccgccggcaa aaacaccacc aagtggtcgt cgccatgctg gacaagggca cgcagctggg
    23821 atggcggcag atcgggcaga agccgcgtgc gcgctgggac aaacacctgc ggtgacactt
    23881 ccgcgaaaag ctgcccggca cacaggtgct gcaccgctgc gctgccaaaa cagaacagac
    23941 cggcgggcag tccgaccgca tgcagcgaaa aaatcgcttc gccactgagc aagccgagca
    24001 gacgctgcag cgtggcgagc cggtgccacg gaatcagcgt tgcgctcagc ggatcttgca
    24061 aagccgaatg ggcagcgaca agccgcagcg tcagtttgag cggcggcggt ggagacgtgc
    24121 tgctgccaag tcgtaccgct tgcagtggct gcgcggcggc gtcgtcttca ccgggcgggg
    24181 caatctgcgg tgtgacaaac cgtccaagcg gcgtcttgct gggtgatttc gcgcgcagcg
    24241 atccgcgccg tccggccaga aacagataca gatccgcggc tggaaacaag ctctgcatcg
    24301 acgtcagccg cagcgggtgg gcatgaccca gatcgatcag aaaattgctg ccttcccggc
    24361 gcagaagacg aaccgaaggc agtgtcaaaa ggcgcagaag aagcgacagg ggaggcgtga
    24421 caagccgcac gatcggcgcg cggaagattc gatcgcttgg cagaccttcg ggtgacggca
    24481 gtggatcggg aatcagcaga tcgcccgatg cgccggtctg ccacagatag cggaccagcc
    24541 gcggcagcag cgtcgtgcca cacgtcagat agatttcgtc gatcggctcg ctgacagccg
    24601 ggctgtcgtc tgggctttgt ggctgcgcaa gctcacgcag aagcccaccg ggcagtgtca
    24661 aaagctgaag ctgctcgact agatcgcgca gcgacagctc tttgattttt cgacacggct
    24721 gcaccagcgt cggggtgtag atcgccagat cgccgggttc tgcaagcgga gttgtcgcgt
    24781 catacccgag cggcgcagcg tcatcgcgat aggcgacaaa gtgtcggccc gcaccgacgc
    24841 agagtggcgc cgacagaagc cgggcaagcc gcgccacccg atccagcagc agcgagtcac
    24901 ggcactgaaa acgcaagaga tacagcgtcg agccgagcgg atttttgcaa agctcgatgc
    24961 gcagcgtggg caccaggtcg tcaagcggca gttcctcgct gagcagccgg gtaagtgaca
    25021 ccactcgctc gatgctgagc aggacgacga ccgcttctgt accgaccacc acaccgcgac
    25081 cgtcttggaa cagaccggcg attcgaaagc tgagctggtt tttggcgacg ggctcaagca
    25141 tcggcaaagg gcgaggatat cagcatccat gataaaacag agcccatggt acaaacggcg
    25201 cgtttgtgtt gtttccgacg acgctttggt ttgcttacct ggctgctggg cggttttttg
    25261 gccgcatgca gcgcgccacc atcggttggt cgcgtggccg ctccggtcgc tgatgttctg
    25321 ccggatcact atcttttgac ggtggatgcg ccgatgagcg gtgcgacgca gaaatcgctg
    25381 cgcgacaaag tggcccagct tgggctggcg gtggaatacg aattttcaga ccgggtaggg
    25441 cttgcagggg gcctgagcgt gcgcggcgag ctggaagcga tccaggcgct tcagtcggcg
    25501 tttccctcgc tgcgggtggc gtcggcggtg ctgcgatatc cagcggggac gacgtgcggc
    25561 aatggcagct gcgaaaccga cgaagcggcg agcagcgacc gcggcagctt gtgcagcctc
    25621 gactgtggtg cccagccact tcgacctgtg cgcgacgaac tgaccaacag ttttttcgtc
    25681 cgccaagtcg gcgccgacca ggtctggccg aaaagccaag gtgccggcgt atcggtctgc
    25741 atcatcgaca ctggcttcga ccgcggggcg atgtcgacgc atcccgaccg tccgcaagct
    25801 ttgggcgagg gctatagctt cgtccgtcaa tcggcggact atgccgatgt ttcgcagcac
    25861 ggcacccacg tcgctggaat cgtcgcagcg gcgaaaaatg gcacaggttc ggtgggggtg
    25921 gctccgcaag cgacggtgcg aaatttccaa gtctttcggc tcaaaaatgg cagcccggtc
    25981 gcaagtgatg ccgacgtgat tgccgcgctg gatgcagcga ttggcgctgg ctgtcagatc
    26041 gtcaacctga gcctgggcgg tgccagcgac agcgaagccg aacacgcggc gatagcccac
    26101 gcctatcagt ccggcattct ggtcgtggca gcggcgggca atggtgaaga tgcggcacac
    26161 ggcgtcgtcg gcaccgcgca aagcaacttt cccgccgcgt acccagagtc atttaccgtc
    26221 ggtgccgtca gtcgcagcga taccttggcg agtttttccg gcaccggccc agcggtcggc
    26281 atcgccgcgc ccggcgtcgg tatgtattcg acagttcccg tgggcactgg ccagcgcgaa
    26341 gtcagcgcgt cgtttgcgca aaatggccga ctccttgcgg tcgatgcgtc gctgccgagc
    26401 ggaagttcgg gaaccagcct gcccgcgacc cgcgtcgtgt cctgcggata cggaagccaa
    26461 agcgacatcg atgcctgtca gcccgcgggg aaggtggcgc ttatccagcg cggtccgggt
    26521 ggtcccggcg aaactgcgat tccgttttac gacaagattc gcgtcgctcg gcagggcggg
    26581 gcgcttgccg tcgtgctgta caaccaccgc tacggtgacc cgagcacggc gggggcgctg
    26641 ctcgaaaaca tcacgattgg cggcgggcag ccggtgatgg tgctgacgct ttcggcggga
    26701 gatggcgaag cgctggcgga aaaggcgacg caagggggac tgaccttgtc ggcaacgtca
    26761 tcgccaagcg actatgcggt gttcgatgga accagcatgg ccgctccggt ggtgtcgggg
    26821 gttgcggcgc ttgtgtggtc ggccaacaag tcgctcggca acgtcggtct gcgccagctg
    26881 ctgtccgaaa gcgcggtcga tctgggagcg ccggggcggg acgatcaatt tggggctgga
    26941 cggatcgatg cgctggctgc catgacagcg ggcagtccgc ggggtctgtg cggcgatggc
    27001 gtccgagatc gcgcaagtga aatgtgcgac ggagcaagcc gcgatggcac aagctgcgac
    27061 gatctgggct acgacggcgt tctttcggga tcgccgggct gtaacgcaag ctgtaccgga
    27121 cttacgcaag gaacctgcgc gtgtgtgccg gggcggacgc cgtttgccgt gacgctaggt
    27181 gtcgatgaaa acgcccaggt gaacggcggc ctgaccggaa cgcgctttcg ctatcacgtc
    27241 gagctggcag gcaagccggt tgctgcggca cgcatcaagg tcacgattgg caaaaacgga
    27301 cggaatcttg tttcctatcg gacgccagcg acggatgcaa acggcaacag ccaggacttt
    27361 gtgccctatg attttaccgg gctttcggcg ggggattatc aggtgatgcc gacggtgagc
    27421 aaaggcgacg gacggtgccg cgacgatcag ccgacgcagc ctgcgcaagt caccgttcac
    27481 attcgcagct agctacttga tgaactcgcg gccccgcaga tagggacgca gcacggcggg
    27541 aacacgaatc gatccgtcgg cctgctgata gttttccaaa atcgcaatca ccgtccgcgg
    27601 cagtgccaga ccggaaccgt tgagcgtgtg gaccaggcgc ggcttgtctt ttttgtcttt
    27661 gccgggacga tagcgaatct gggcccgtcg cgcttggaag tcaccgaagt tcgagcacga
    27721 cgacacttcc agccattcgt ttacgccggg cgaaaacact tcgagatcga acttttcaca
    27781 cgccgcaaac gacaggtcgc cggtgcacat ctggatgacg cggtacggca gctcaagctg
    27841 cttgaggatt tcttcggcgt cggacaaaag cgagcgcagc gcctgtttgc tgtcttcggg
    27901 acggacaaac tgcaccatct cgactttgtc aaactggtgg ccgcgcttca tgccgcgggt
    27961 gtcgcgtcct gccgccattt tttcgcgccg gaagcacgcg gtataggcga cgtggcgaat
    28021 cggaagctgc tcgttttcga gaatctcgtc gcgatagagg tttgtgaccg gcacttcggc
    28081 agtcggcaca aagtagaagt cttcctctgc atcgcggtag aggttgtcgc cgaacttggg
    28141 cagatttccc gtcccgtaca ggcacagttc cttgaccatg tagggcggat agacttcggt
    28201 gtagccctga tcgatgtgga cgtcgagcat ccaggtgatc agcgcacgct gaagcgccgc
    28261 gccggcccct ttgaggatgt agaaccggct gcccgagatt ttgacgccgc gctcgaagtc
    28321 gatgatgccg agcgactcgc cgatttccca gtgcggtttg ggcgcaaatc caaactcaaa
    28381 gcgcttgcct tccgtccgca cgacctggtt ttccgcgtcg ctgcgacccg tcggcacttc
    28441 cgaatcggga aggtttggca gctggagcag caggttgtcg agctgggctt cggtttccga
    28501 aagctgcgca tcaagcccag caatgtcggt tttgaccgat tccatgtctt tgatgagcgc
    28561 ggcgcgcttt tcggcgtcct tttcccgtcc gataagctgc gatgcactgt tgcggcggct
    28621 cttgagctcg tcagcggctt gcagcagatt gcgtcgcttg tcatcgagcc ccagcgccgt
    28681 ctgggctagc tcagcagcgg catcatcgaa cagccgctcg cggtagatcg acagaacctc
    28741 attgggacgt tcacgaagga tttttgcgtc gagcatggag acgcattctt ccctttctac
    28801 gaggccaaag tcaactatcc gcgacgcgct gggggtgtac cttcggcggt ttttgcgaca
    28861 cactgggctc atgaagactc accctgtgcg tggtattttg gctgccagcc tgtttggtct
    28921 gggtttgctg acggtggctg gcggctgtgc ggggtcgctg cagcgcgtac cgctttcggc
    28981 caatctccgg cctagtggac cgccttctgt tcgcaaagcc caggacttgg gcagtctgcc
    29041 gctgcctggc gctggtccgc tgtcgcttgc gcacgcggat gacctgttta ctcccggtga
    29101 gtacgtcgcg ctgtacggcg aaaatctgac gcccggatcg caggtggtgc tcagcggacg
    29161 ggcggcagcg gtggtcggtg cgatcgacgg aggactgctc atccaggtgc cgcgcggaat
    29221 ttcgacgggg cggcaagtcc tgcggatcga caacggcttg gggacggcgg aagtcacggt
    29281 gtcaacggcg ttttatgcgt ttggcggcga cgcggcgggc aacgggctgc ggattcgacg
    29341 gcttgggccc gaaggggatc agccggaaat gttcgacgac aagccgctcg atatcgcctt
    29401 tccgttgtcg cgctatcagg cgctctcata cgacggagca ctgctttttg cgctggccga
    29461 acccgcaacg gacgaagccc cggaatcgag cagcggcgag gcaatccccg tcggaacggt
    29521 ggtgtgcgac ttgatggttg tgcacatggg ggccaaacac gggccacagg aagtgacccg
    29581 cgtgccgctc tctctcgtcg gaaaaccgac cggacttgcg atggggcccg caggtcagct
    29641 cgtcgtgctt cagccgcggc aaatcaccgt ggtcgatgtg tcgataccgc agaaaccgca
    29701 gccggtggtg gtgttgccgc tgtggccggc ggaacagaaa cgcgagctcg tcgacgcaga
    29761 gtttctcgga gacggtcgct tgctggcggt actggaagcg tatgcgaacc agattcacct
    29821 gatcgacgtc ggtgatccac gccagccgcg acttgtatcg ggggtgtcgc tgtcgcccga
    29881 atatgaacag ccgttttcga tcgacttggc acccggtccc gacccgtcgt cgatgtgggt
    29941 cttgcaagga ccgaatttgc ggctggcggg aaagcgactg ctcgacgggc tttcaggggc
    30001 atggcgcgat gccaaggcgc tggatgtaaa gggcgcggcc aagacgctgg gacaaaccgc
    30061 ggtcggcgct gcgatgctgc cgtcggaacc gctgacgcgg gtgcagcttg tgcagcttgt
    30121 cggcggacag cttcaggtgg tcaagtcgct gccgttgccg tcggaaatat tcccgttttt
    30181 catccaaccg gactatcgcg gctcgctgtt tgtaagcggg gtcaatcgca aaaatcagtt
    30241 cagctccatc gacgcctcgc tcgacggtgt gcgccggctg cttggtgcga tcaaagacac
    30301 gacccagctt ggcgtgatcc tcaaaatggg gacggaagat ggatcggtag cgacggcggt
    30361 gcagggcgtg gcgatgtact acgatttggc gctgcttccg tcgggacagc ttatgacttc
    30421 gacgatccga ctggggcctg gctacattcc gccgcgggtg acgctcgact ggggattcga
    30481 ggtgcccggc aagtcgtttg cgaagctgcg cgaggtcgcc aacaccggtt tcaagatcac
    30541 cgatgtggtg cgccggctgc ttccgcccta tcgctatgag aggataggtg ttcaataagc
    30601 gtggtcgacg gcggtgcgcg acaatgagtg gcgaaaccgc agccaagttg tgtaacgtct
    30661 tgagacatgc cgacagtgga ctttgatttc cagcgctatg ttgctcgcaa aaaaggcgcg
    30721 cgtgcggcgc aggttcgcga gggtgcggcg tatgcctatc ccggtgatgt tcgacttttg
    30781 cgcacgcttg accggcttcg gcccgtcgaa ctggcgctgc ttgaagccgg gcgcttgtgg
    30841 cagagcgtgg cgcgggccga gctgctcggt ccagcggtgc gggcggtggc gccacggttt
    30901 ggtcaagtgg cgacggcagc caagcgctgc gccgacatcc tgcacatcga cgtgccgacg
    30961 atctacatcg cgccgcgtcc gtcgggtgtg ctgacactgg gaaccgaggc tgagacctac
    31021 atcgtgctca gccagggact gctcgatagc ctaagtcccg acgagctttt ggatgtgttg
    31081 ggtcgcgagt gcggtcggat tcaaaacggg catgtgccat tccggactgc gctgcactat
    31141 ctgcaacaca acgccagcac gatgacccgg tgggcggttc gtccggcgac gctgacgctg
    31201 ctcgcttggg ctcgtcgcgc tgatatcact gcggatcgcg cgggtgtgct gtgtgcacgc
    31261 agcgtcgacg tgtcgctggt cgcgatgcag aaagcgtttc ccgaggacga cagccgcgag
    31321 cttgcccggc ggtatcgggc gatccagcgc tttgttggca gcttgtatta ccgcaccgtc
    31381 gtcggtcagg atgccgaaga tggcgagagt accgaagact gtgatgctgc ggtggcgcag
    31441 attctcgacg acaaagaggt tccgccgcgc acggcacgcg gggctgctgc ggcggcttct
    31501 ggggaatcga gtggtgaggc ggggcaggga ggcagtcgat gagcggactt accaagcagc
    31561 atcgccagcg caaagacgaa ctgctgtcgg cgctgcgtga agtggcgcgc ctagccgacg
    31621 aaacgggcat gcacacgctg acgtcggagc tgctgcgcga acggattccc aagctcgaag
    31681 aggaacgctt tgtcctcgtc gtgcttggcg agttcaatca tggcaaatcg acgtttgtca
    31741 acgcgctgct cggcgcgagt gtgttgccag cggggattac cccgacgacg gcgacgatca
    31801 atcacatcgt ctatgccgac aagccgtgcg ccaaagcggt gctgctcgac gaaacccagc
    31861 gtgtcgtcga tcccaaatcg ctgaacgaat gggtcacgat cgagggcagc aaggccgaaa
    31921 agacccgcta cgtcgaggtg ggctggcccg ccgacatcct gcgcgaccga gtgacgctcg
    31981 tcgacacgcc gggggtcaat gacatcaacg aagcgcgcgc cgagattacc tacaattaca
    32041 ttccgcgcgc cgacgcagtg ctgtttcttc tcgacagctc gcaggtgctc aagcagtcag
    32101 agcggtcgtt tttggaacag cggattttgc gtcgcagccg cgacaaactg ctgttcgtga
    32161 tcggcaagtc ggatctgctg tcgccagagg agcgagccga agccacgcgc tatgtccgcg
    32221 acaacttgca gcctctgctc ggggcggaac cgatgatttt tccggtgtcg gcgcggcttc
    32281 agctggctgg gcgcagcgac gaaagcggca ttccggaact gaagagctat ttgcagcatt
    32341 atctggccga cgagcgtgga cgggtgctgc tcgacaatgc aagctccgac ggacaaaggc
    32401 tgtgtgccta tctgcggcaa aacctgggca tcaaagcgcg ttcgctgacg ctgtctttgg
    32461 aagaactgga agcgcggatt gcgcgggtgc aaaaagagct gtcgggcaag cagcagacga
    32521 tgcggcaact gcaagcgcgg atcgtcgctg agtccgatgc gataaaggcg cggattcggc
    32581 tcgatctgga agagtttgtc gccgcgtttt cgagcgcgct gccgggcgag attgacaagg
    32641 caagcagcga cgacgtgcgc aagtatttgc agctgttttt gcaagacacg ttcaagcagt
    32701 gggccgagca ggaaggcgac cgcgtggcgt cggagctaga gcggctggcc gagcagatca
    32761 tccaaatcac caacgaaaac gtccagcagg cgatggcaac gctggccgcc gagcttggtc
    32821 cgacggatac ccgcgtcgac ttgcaggtgg ataccctcaa gtacgatgtc ggcatctttg
    32881 cgctgggcgc gctggggacg ggaattttcc tgtttgtgaa cagctttgtc ggtgggcttc
    32941 tgacgctggc ggctccgatt atcgcggtgc tcatccacag tcggatggcc gacgaaatca
    33001 agcgccaggc caagcagcaa gcgccgcagg tggtgcagac ggcagcgcag gcgatgctgc
    33061 cgcgctttca gcagatcgtc gacgattttg ccgcgcggct cgcggacttt gtgacggcgg
    33121 cgggacaggc actgcaccgc gggatctcgg aaatgctgga tcgggcgctg gccgagcgca
    33181 aagcgcaggg gctcgacgtg gccgttcgac agcgtgaaat cgacgcgcaa gtgaccgagc
    33241 ttcaccaggt cgagcagcga ctttcgacga ttcgcgaagc gctgtgggcg ccagccgaag
    33301 aaggtgatgg ggcggacgcg gtggacccgc aatgatcacc gtcgagctcg tcccgtgtta
    33361 cagcgacaac tatgcgtatt tgctgtcaca tccggattcg gcggatgtgg tcgtcgtcga
    33421 tgcgtgtgag tacgcaccga tagcggcggc gcttggtggc cggcggctgt cggcgatttt
    33481 gtcgacgcac catcatcttg atcacgtcgg tggcaatgaa gagtttgcgg cgcagtttcc
    33541 ggggctgccg attttgggac acagtcagga gctggtcgac gggcgcatcc ccagacaaac
    33601 ggcgggtctt gccgacggag aatcgttttc gctgctcggc agccgcgtgg tggcgctgca
    33661 tgtccctggt catacgctga ctgccgtggc gtaccacttc acggacgaag gcatcgtgtt
    33721 taccggcgat acactgtttg gcgccggttg tggccgactg ttcgagggaa ccccagtgca
    33781 gatgcatgcc tcgctgtcgc gacttgcggc gctgactccg tcgaccaaag tctatagcgg
    33841 acacgaatat ttggcgcgca acctgggctt tgcccgttcc gtcgatcctg gcaatcaggc
    33901 gacgcaggat cgcgaacaag actgccagac acgacgcgct cgccacgaac cgacggagcc
    33961 gtcgagcatt gcgctggaac ggagcacaaa tccgtttttg cggacgtccg agccggcggt
    34021 ccaagcggcg gtgcaaagcc gtcccgtcga aaccgaagac gctctatcgg cagtcgaggt
    34081 gtttgctcgt ttgcgacggt ggcgcaacac attttagctg ggcagcacag gctggctcgg
    34141 cggggaggac gcagcgatga ttcgtcgctt cgctgagatt tcggcgcacg atctgcccaa
    34201 agtaggcggc aagggcgcaa acctgggcgt gctgacccgc gcgggctttc cggtgccgcc
    34261 gggtttttgc atcacgacgg acggatttgc agcctttgtg gccggatgtg ccgaactccc
    34321 gacgctgttt gccgatctgg atgcgctgcc cgtcgatgat gtcgaagccg tccgcgtggt
    34381 gggggcgaag gttcgggcgg cgctgcttgc ggtgccactg ccagcagcgg ttgtcgatgg
    34441 cgcggtggcg gcctggaaag tgattggcga cggatatgcc tatgccgtgc ggtcgagtgc
    34501 gacggcagaa gacctgccag gtgcttcgtt tgcgggccag caagatacct atctgaacat
    34561 tcgtggagaa gcggcgctga tcgatgcgat ccgtcgctgt ttcgtgagtc tgtttaccga
    34621 tcgcgccatt ttgtatcgtc agaaaaaccg ctttggccat cgcgggattg cgctgtcggt
    34681 ggttgtccag cggatggtgt ttcccgatgc tgcgggcatc ctatttaccg ccgatccggt
    34741 gagcggccat cgcggcacca ttgccatcga cgcgggcttt ggactcggtg aagcgctggt
    34801 gagtggcctt gtgaccgccg acctgtaccg catcgacaaa gcgaccggcg ctgtgaagga
    34861 agttcgagtc ggtgacaaag cgattgcgat tcgcgccctg ccggctggtg gcaccgtgac
    34921 cgaaccgctc tcgccgtcgc agcgacaggc gcgggtactc gacgatgcgt cgctgcgcaa
    34981 gctgtgggat ttggcgcggc gcgttgagtc gcactacgac ggtgtgcccc aggatgtgga
    35041 gtggtgtctg gaatgcgatg atctgtttgt tgtccaggct cgaccgatca cgtcgctgta
    35101 tccactgccg acgccgacac cgggccgcgg gctgcgggtc tattttggct ttggccacgc
    35161 gcaaaacatg attgcgccga tgagtccgct cgggctcgac atctggcgga cggtgtttcc
    35221 aatcggcaag cgtgagctaa ccgaagtcat cgaagaaccg acggtgatgg tgacggcagg
    35281 cggacgactg tacatcgacg tgacggcacc gctgtcgacg ccgcggctgc gcacggcact
    35341 gctgggggcg ctgggcgcga tctatccgga catcgcgcgg ctgctggctg agttggcgca
    35401 gaggcccgag attgcgacgg cagattcccc aagcctgcgc agctttgtgc cgctgtggac
    35461 gcttctgggt ggtgtgccgc cgcgtctgct caaggagctg ctttggaatg atccgcagcg
    35521 gctgcggacc gaactggaag cgtgcatcga cgaaaaactc agccactttc gccgcgaaac
    35581 cgagcagctt gcaccgggcc tggcccgtct tgcccgtttg cgttcgctga tgtcgtcgat
    35641 gtttgggatc ttgcagcact ttgtcccgcg gtttggctcg gcgatgctgt cactgcggct
    35701 tttgacccag cgctttgccg atacagtcca tgccgccgac gtcggggcga ttcagcgtgg
    35761 ggtctatggc aacgtgacca ccgaaatgga tctcctcgtc ggtgatctaa cggatttggt
    35821 gcgaccgcat cccgaactcg gtgcggcact gcgtctcgcc ggctcgcagc cgttttcgcg
    35881 agagtcgctg gccggtctgc ccggcggtgc ggcgtttgtg gaagcgctcg accagttttt
    35941 gcatcgcttt ggcatgcgcg gaccggggga aatcgacatc gcgcgaccgc gctatcgcga
    36001 tcagccgggg cttctgtttt cgtcgatcat cggcggactg tcaggctcgc agccgggggc
    36061 tcatcgcgcc cactttgcgc gtctgcaaaa agaaggcgac atggccggcg agcggctgat
    36121 tgcggcggcg ggtggcccgc tggcgcgccg attggttgcg cggcaggttg gctgtgttcg
    36181 ttacgggctt gggctgcgcg aacatccgaa atatttcctc gtgcgctgtc ttgatgtgct
    36241 gcggaccgaa gtgctctttg cggcccgtga gcttgtcgcg gccaagcggc tcgattccgt
    36301 cgacgacgtg tggcttttgc gctatgcgga gctgttgcaa cttttgcaaa aaccgacgct
    36361 cgatctgcgc catgtggtcg ctcagcgacg gtccgactat gttcgctacg cgcagctcca
    36421 gccgccgttg gtgatgacca gcgacggtga aattccgcgt ctgcccccac ccaaagacct
    36481 gccccaaggc gcgctcgctg ggcttggcgc gtctgccggc gtggtcgaag gtctggcgcg
    36541 cgtggtcctc gaccctgccc aagatgtctt acacgctggc gagatcctgg tcgcgccgta
    36601 caccgatccc ggctggacgc cgctgtttgt gcacgccaag ggccttgtct gcgaggtcgg
    36661 cggcatgatg acgcacggat cggtggtggc gcgcgaatac ggaattcctg ccgtcgtcgg
    36721 cgtcagctcg gccacacggc tcatccgcag cggtcagcgc attcgcatag acggcagccg
    36781 cggactggtc gaaatcatcg acgatcagca ggtcccgatg gcttcgtctt cgtcgggaag
    36841 ataggcaaac gttcgcacca gccggacgag ctgctcacgc tgggcatcgc cgaggtcgac
    36901 aaactggatg cccattccgg gctggcggct ttcatcgtca tcgttgcgcg gcgggttgat
    36961 ccagatcacc tcgccttcga gatccatcgc gggaccgccc ggcgggctaa agcgcaggtt
    37021 cagccgcgcc cctggtggtt ccggcgtgcg cgtgcgaacg aaaattccca tcgcgctgat
    37081 gtcggaaatg tacgcgaaca aaaacgtgtc cgacgcccgg taatcgacgg cccaatcgac
    37141 gaggattcgt tcgtggacgc gacgatccgc gccgctccgc cgatcgacga gcccatctgg
    37201 gccgggtcgc tcggtttccc gacggtcctt ggctggctgc gggggtctgt tcattgcgcg
    37261 atgtgtcctc agaaagcgaa agttccgcct tccatacagc gccgggacct gcgcgggaag
    37321 ttttttttgc ccggaccgct tttcgggatt ggctaagcta gccgccatgt ctgcccgccg
    37381 tcgtcgctct gaaccgtctt cgcccgtcgc tccgccggat gccgcacctg ctgtgcccga
    37441 cgatggagct gcgtccgctg tgcccgcggc aaaggaagtg gcgacggcac acgttgaacc
    37501 cacgagcgac acaaccccca ttccatcacc agaaggtgag ccgcgccgcc ggggtcgtcg
    37561 tcgtgaagtg ccggttttga cggaagacga tctgctggtg cgtccgtcga ttgccgagct
    37621 gctcgacaag atgccgaccg atcccggcgt ctatctgatg aaagacaagc gcgggcgggt
    37681 catttacgtc ggcaaagcca aaaacctgcg caaccgcgtc cgcaattatt tccatcgctc
    37741 gggcgacgac agaccgtttg tgaagctgct cgatcggatt ttgtccgaca tcgaaacggt
    37801 gatcaccagc agtgaaaaag aagcgctgct gctcgaaaac accctgatca agcagcacaa
    37861 gccgcggttt aacgtccggc tcgtcgacga caaaaactac ttggtgctgc ggctcgatcc
    37921 gaaagcgcgc tttccacggc tcgaagtgac gcgcaaaatc cgcgaagacg gcgcgcgcta
    37981 ttttggcccg tatcactcgg cgcgggcgtg tcggcagacc ctggaggtga tcgggcggca
    38041 ctttcggctg cgcacctgta ccgatcaagt gatgaacagc cggcgcaggc cgtgtctgga
    38101 ataccaaatc aagcgctgcg acgcgccgtg tgtgctgccg attccgccgt cgcaatatgc
    38161 cgagcaggtg cgggacgtcg cgctgttttt ggaacacaag tcgagcgagc tgctcgacag
    38221 gctgcgcagc cggatgatga cagcgtcgga cgaactggaa tttgaaaccg cggcagcgct
    38281 gcgtgatcag ctcaaagcga ttgagatcac gctcgaacga cagcatgcgg tggcgaccca
    38341 gtttgtcgat caagatgtgg tcggtttcta tcgcgaaggc gatgtactgg aaatcgcggt
    38401 gctgctgatt cggcaaggca agctcgtcgg aaggcggacg tttcgctgca ccggccagga
    38461 atttcccgac gaggaatcgc tttcgagctt tgtcagccag tattacgacc ggcccggcat
    38521 cgaaatcccg gacgaagtgc tgcttcctct cgaaatcgag gacgcccagg ccaaaatgga
    38581 ctggctgcgc gagcgcacca tcgagctgtt tgggcgcgcc cgcaaagtcg aagtgctcac
    38641 gcccaagcgc ggtcctcgtc acaggctggt cgatctggcg agcaagaacg ccgcggcggc
    38701 ctacgcgagc cggcgcaacc gcacccgcga taccgaagcg tcgctgcaaa aactccagcg
    38761 acgactgggg ctgaaaaaac tgccggcgcg catcgagtgt ttcgacattt cgcacttgca
    38821 agggtcggac accgtggcct cgcgggtggt gtttctcgac ggggaaccgg ccaaacatct
    38881 gtaccgcaca ttcaaagttc gatcggtcga aaacgatgac tttgccgcga tgtatgaagt
    38941 ggtgtcgcgc aggttccgtc gcgcgcttgg caaagaaccg gggcaaaccg atgcgggcgg
    39001 cgaggtcggc ttttcatcgg cacaagtggc gaggcgacga tcggcggcgc tactccccgt
    39061 gagcattgca agccctgcgg cggtggtgcc acctgcggaa gatgcggaag ttcccgatgt
    39121 cgatgatctg gaagccgccg atgcccaggt cgaagccgcg ctcggtgatg cggatgcttt
    39181 tgccaatgac gatgatgagg cgcgggccgc tgacccttgg gctttgcccg atctgctggt
    39241 gatcgatggt ggcaaaggtc agcttgcgtc ggcgctggcg gcgctcaaag acgtgggcat
    39301 caactgggcg cagcagctcg atgccattgg tctggccaaa gagcgcgaag atcagagcgg
    39361 caaccgcaat cccgaccggg tgtttctgcc gcgcaccaaa gatccgatcc ggctgcgtga
    39421 caacaccgca gagctgttcg tgctgtcgcg gattcgcgat gaagcgcatc gcttcgcggt
    39481 gagctttcac caaaagctgc gggaaaaacg gacgattcga tcgcagctgc aagagattcc
    39541 gggcatcggg cccaagcgac agcaggcgct gctcaagtcg ctcggcagtg tgcggcggat
    39601 tcgcagcgcg agccgggacg aactgctggc cgtgccgggg atgagcgaag cggcggtgca
    39661 agcggtgctg cattttttcc gcgccgaccg gcaggccgat ggcccagcgc tgccagcgtc
    39721 cgagatcgct tcgcagatcc ccaaaagcag tccgccagcg acgacagatc gccaagaaat
    39781 acgccgcgaa actgcgcctg tggcaacccc cgatgtgatc gaaaacgcgg ctgcggcaga
    39841 gctgagcgca cttttgaccg acgaaagcga agacgatctc gagcgatcgg tggctgccgt
    39901 tatcgcggaa gaagcgggtg gcaaaggccg cgaattgggc gggtaaattc gcaattggtc
    39961 gggtggttaa cctctcagct tcggaaggct ttcccgtcgc ccacacgcaa cttagcgggc
    40021 gcgagcgttt gaggaattgc actgcgttac cctacatgct aagtagcgat cctcttgtcg
    40081 cgctggcggt ccgaaattcg ctttggctta gattttttcg tcgacgcggt gagggaaaag
    40141 cagcacctga atcggtaaca caactttttt cagctcgacg gggatgcgag ctgaggcgct
    40201 tggcctgtag ctagccactt gggcatccta ttgtccgacg gcgacctgcg ggtgcaacgg
    40261 ccatattgag gaatgagcag atgatggaga agtcggaagc agcggtcatt gtggggcgcg
    40321 cccaaaccta tggaagcggg agccggacgg tgagcaaggc caagcaagcc agtgaatcga
    40381 agtcgaccaa aaagcgggcg ccgagcaaat cgagcaaagc cgccgacgct cgggcgagca
    40441 agccgggtct ccagattcct cgctatttca gcaccgcagg ccgcgatcca tttggcgaag
    40501 tcgagtggga gctgcgcaac gccagcatca ccggcgagag cggcaaggtc tatttcgagc
    40561 aaaaagacgt cgagttcccc aagtcgtggt cgcagctcgc gacgaacgtg gtggtgcaga
    40621 agtatttccg cggcgcgatg ggcacaccga cgcgagagcg ctcggttcgt cagctgattg
    40681 gccgcgtggt gtcgacgatt accggctggg gtctcaaaga cggctacttc cgcaccgaac
    40741 aggacgccga agtgtttcgc tccgagctga cccatctgct cattcatcag aagatggcgt
    40801 tcaattcgcc ggtctggttt aacgtcggtg tcgaaaccga gccgcagtgc tcggcctgct
    40861 tcataaacag tgtgcaggac acgatggaca gcatcctgac tttggccaag accgaaggca
    40921 tgctgttcaa gtttggatcg ggcaccggaa ccaatttttc gtcgatccgc agctcgcgag
    40981 agtcgctgca aggcggcggc ggcgcatcgg gcccggtttc ctttatgaag ggcttcgatg
    41041 cattcgcagg cgtcatcaag tcgggtggca agacccgtcg cgccgccaag atggtcattc
    41101 tgaacgccga ccatcccgac atcctcgaat acatccactg caagtccagc gaggaaaaga
    41161 aggcctgggc gctcatcgac gcgggctacg acggcagctt caatggtgaa gcctacaact
    41221 cgatcttttt ccagaacagc aacaactcgg tccgcgtgac cgactcgttt atggaatcgg
    41281 tggtccacga tcgaccgtgg cacacccgcg cggtgcgtga cggatcggtg gtcgattcct
    41341 atcgggcgcg ggacatttgg cggcaaattg ccgaggctgc gcaccagtgc ggcgatccgg
    41401 gtcttcagtt tgataccacc gtcaattcgt ggcatccgtg cccgaactcc ggtcggatca
    41461 atgcgtcgaa tccgtgctcg gaatacatgt tcctcgacga cagtgcttgc aacctggcga
    41521 gcctgaacct gcgcaagttc cagttcccag tcggtcatcc gcaagcgggt gactttgaca
    41581 tcgagtcgtt cgagcgcgcg gtcgacctga cgattctggc gcaggaaatc atcgtcgaca
    41641 acgcccgcta tccgaccgac aaaatcggtg aaaacagcca cctgtatcgg cctcttggac
    41701 tgggttatgc aaacctgggc gcgctcctca tgtcgcgggg tctgccgtat gactccgatg
    41761 cgggtcgggc gtatgccggg gcgattaccg ccatcatggg tggtcgggcg taccggatgt
    41821 ccgccgagat ttcccgcgac cacggcggac cgtttgccgg atatgaaaaa aaccgcgagc
    41881 cgttccttgg cgtcatgcgc aagcaccgcc agcacgtcga ccacatcgac tcggcgctgt
    41941 gcccgtatga cctgttgcaa gcggcgcgcg cggcgtggga tgaagccatc ggcattggcg
    42001 aacaccacgg cttccgcaac ggccagtcca ccgtgctggc tccgacggga acgatcggct
    42061 tcatgatgga ctgcgatacc accggaattg aaccggacat cgcgctcgtc aagtacaagc
    42121 ggctcgtcgg tggcggtctg ctcaagatca caaatcagac cgtgggcgaa gcgctgaccc
    42181 gtctgggcta cgacgcgact gcgcagaaac agatcaccga cttcatcgcg acccacgaca
    42241 ccatcgaagg cgcgtcgggc ctgcgacccg agcatctgtc ggtgttcgac tgcgcgttcc
    42301 gggcccaaaa cggcacccgc tcaatccatc cgatgggtca catcaagatg atggcggcgg
    42361 cacagccgtt tatctcgggc gcgatcagca agacggtcaa tctgccgagc gacgcgacga
    42421 tcgaagacat cgaaaacgcc tatatgcagg cgtggcagct tgggctcaaa gcgattgcga
    42481 tttatcgcga tggctgcaag cgcagtcagc cgctgtcgac gtcgaagacc gaaaagaccc
    42541 agggtccggc tgcggccagc ggatcgtttt tgccggtgca ggtgctgatc gacaagctgc
    42601 ccaccgaaat tcagcagcag cttcgtgatg cgacctcgct gtcgctggaa gacttcctgc
    42661 agcacgttgc cagcctggcc aatccgaaag gccagccgat ggcggtgcgg cgcaagcttc
    42721 ccgacgagcg acggtcgatc acccacaagt tcgcgatcgc tggccacgac ggctacatcc
    42781 acgtcggcat gtacgacgat ggctcgccgg gcgagatttt cattcggatg gcgaaagaag
    42841 gatcgacgat ttcgggtctt atggactcgt ttgcgacggc ggtgtcgctt gcgcttcagc
    42901 atggcgtgcc gctcaagctg ctcgtcgaca agttcagccg cacgcgcttt gagccgtcag
    42961 gctggaccgg caattccgaa attccgcgcg ccagctcgat catggactat ctgttccgct
    43021 ggctcgaagc gaagtttttg ccagagcagg ttcaaggcga acagctagag atggatctgc
    43081 cgacgccggc tccgactgcg cagtcggtgg atgcgccgag cccgacgctg ccgaacttgg
    43141 gttcgatcat cgcagcgacg gcggcccgcg tgttccggac cgaaaccgat gcgccgacct
    43201 gccacgagtg cggcacgatc atggtgcgct cgggggcctg ccacaagtgt ctcaactgcg
    43261 gcgccacgtc cggttgtagc taagctttcc gcgattcctg tccgattccc ccacaatgga
    43321 atgtggtcgc cacgcggtgt ggcggcgcgt gcgtatggta gtcaatatta gtcatgtaaa
    43381 atgactcata ttcgacgacc tgcgcagcat gccgcgcttc tgctacgata acggtccttg
    43441 cttgaacggg aggacgacaa ggtgagtact gtgacgaaac cgtggagcct tgactcggtg
    43501 gtggcgcagc tacggcaggc gcggcaagtt ccggcggcaa gccgacgctg ctgtggtgac
    43561 aaagaaccac ttccctcgcg tgaacggctg gtccatgtcg tgacttcgct gcgctcggcg
    43621 ctgtttcctg cccactttgg tccggcggag ctgtctttgg atgcggtcga tcatttcgtc
    43681 ggctacacgc tcgatcgcgc gctgcatacc ctgcgtgaac agattcggcg cgggctcatg
    43741 ttcgcaggat cgtgcggaga cgaagcctcg ctgcgggccg aaaaaatcac ccagcagttt
    43801 gccagtgagc tgccggcgat tcacgagctg ctccagtcgg atgtgcgcgc cgcgtatgcc
    43861 ggtgatcccg ctgcaacaag tcccgacgaa gcgatctttt gttatccggg gatcacggcg
    43921 attacccatc accgcctggc gcacgcgctg catcggcttg gggtgccgct catcccgcgg
    43981 attttgtcgg agatcgccca cgccgaaacc ggaatcgaca ttcaccctgg cgcacagatc
    44041 ggcggcagct ttttcatcga tcacggaacg ggcgtggtga ttggcgaaac cagtgtcctg
    44101 ggtgagcgcg tgcgcatcta ccaaggtgta acgcttggcg ccaagagctt tcctgtcgat
    44161 gaacatggga atcccaaaaa gggaattccg cgccatccgg tggtggaaga cgatgtggtg
    44221 atttattccg gcgccaccat tttgggacgc atcaccatcg gtcggggatc gagcatcggc
    44281 ggcaatgtct ggctgacccg cagcgtgccg ccaaacagtc ggatcaccca agcgcaagcc
    44341 agcagcgaag cgtttgtcga cgggtccggc atctgatcaa aacagccgtt tcgttttttc
    44401 aaacgctgca ccggcccgtc cagattactt gcgcgtgatc gtcaggctgc cgatggcggc
    44461 gcctgccgac agacctttgg acacgccgac caagtacagc tcgtgatagc tcggatcgct
    44521 cgccgacaga ccttttttgg tcagcttggt gatgttgatg ctttcgccac cagcggtcgc
    44581 accgcgatca ttgccgctgt attccccacc aaacgccgcc gcatggcgga gtcccaagat
    44641 cacaccgaag ccccactctg aaccgccgcc gacctgcgcg ccaaaggtca cgctcttcag
    44701 ctccagtgcg accttgggtt ccgacgaaga aaggtgcgcg agcccttgtc cttttttcac
    44761 tttgaccagg aatccgcctt ctcccatttg ataggcaaag attccggtca cagttgcttc
    44821 tttggaaaac gcggcaatct ttccgctttc aaatgcggtg cgcagcctgc caagtgcgat
    44881 gtcgcgacgc tcggctggca ttcccagttc atccccgagc ttgcgcgcgt gtccctccat
    44941 cgcggaatac gcgcgggtcg atacggtttc tggttcgacc gatggcggct tggccagcgt
    45001 cggggaaacg gtcagcagca gagaaccgag cacgatgaaa cggaaaggtg aattgcgcat
    45061 ggtgaaaagc atacatctag cgcggcagtt cgaagtgaaa ggtgtagagg gatggtgtaa
    45121 tcgacgggtt ttccatcgct gccgatcgcg ggactaaact tgnnnnnnnn nnnnnnnnnn
    45181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45241 nnnnnnnnnn nnnnnnnnnn nncacgccgc cacttgccaa acgaccacga atcgtcagcg
    45301 caagcgactg cgcgctggga agggagctgc gccgcatggc cgagcgtgcc gccgatgaaa
    45361 gcggtgccga ttgctatggt tgtttttgca agaaaggctg aagccgcttc cgcagcgtgg
    45421 caacgacttg tggtttctgc gccgcgatat ttttctgttg cgtcggatcg ctgcccagat
    45481 cgaaaagttc attcaggttg tcggtgaact tggcgtagag cgcgtattcg accccatcga
    45541 tcgtatcgac aatcacgcgc tcatttttgg gccacgccgt acagggcagc atttcggcaa
    45601 tcgccggcag tggcgccggc agcttgtcgc ctttcaaaag gggcagcagc gattgaccgc
    45661 ggaacgaagc cggtgccttg cgccccgaca gatcgagcag cgtcggcgcc agatcgataa
    45721 gtcgcacgcg atcggcaatt acccgcggtg cgagccccgc ttttttcggc agatatacga
    45781 tcagcggaac tttcagcact tcgttataga gcgactcgcc gtggaagtag agcggctcac
    45841 cgctgagctt gtgttcgcca aacgcttcgc cgtgatcaga aaacaccacg acggcggtgt
    45901 cttccgaaag ccccgcttcc gccaaggccg ccagcatttt gccgatgtgc acatcggtga
    45961 aactgacctc tgcgtcgtac cgttccttga tgaacttgaa gccttttccg acgggaaatt
    46021 ccggatgatc catgtacgtg ctgtgcggtc caaacagatg cgtccacagc acaaacttgc
    46081 gatccggctt ggactctttg ccgatcgctt tgatttttgc caccacctgc gccgtgattc
    46141 gcggttccgg aatgtcgctg tttgagtcgt gcaggttcgt cgctgcgtcg tttttgtatt
    46201 catcaaagcc caggtggagc ccggttttcc gatccagata gaagtgagaa aaaattccca
    46261 ccgtatgcag ccccgccgac ttgaattcgg taaacagcgt cggatctttt ccggtcagcg
    46321 gcgaaaaatt gagcgactgt ttcacgaagt gaacatccga cggcagccgt ccggtcaaaa
    46381 acgatgggaa gctgcgtggc gtgttcgggg cttgggcata ggcgttgcca aactggaatc
    46441 cttcggctgc gagcttggcc agcgtcggtg tggtcttggg atgtatccga tcggcacgca
    46501 gcgtatcgac cgtgatcacc agccagttgc cattccacac tgcggcggac tttgcagccg
    46561 gttcgggtat gaccggcgtg ttggtctgcg caggttcagg tggcgccggc gcttccacga
    46621 tcttttccgc atcggcgcca tcgcagtctt catcgattcc atttcccggt tcgtcatcgg
    46681 caccgggatg aacgcttgca tcatgatcgt tgcagtcgcc acccccgagc cgtcccgcgt
    46741 atccatcatg atcgcgatcg aaaaagcgcc gcacaagaag cagcagcggt cggcctgccg
    46801 cggtttcttc ggcaatcagc gcgagcgagc gtggctcact gccgaacttc tgcaccgacg
    46861 caaaaaagct cacgatgatg agcaaaagcg cgcccattcc gacaagctgc ggacgcggca
    46921 ggcgcgcaag cgaagtcgcc aaaatcaaca gcgccacgct ccacaccgcc aacactttcc
    46981 atgggccaaa gtggatgatg cgccagtcga cagacaggat cgccagcgca atcaccagca
    47041 ccgccgccag catcgacaga ccgcacagcc acagcacccg cggacgcggc aaaagccagc
    47101 cgacgatttg atgggtgatg cgatacagcg gcaccgaaaa cggcaaaagc agcaccagcg
    47161 tcgccagcga gaccagcgcg gaagacagtg cgccgttgcg tttgttggcc atttcaagcg
    47221 ccgctgtttt gacgtaaccg tataggactg tcgcgtcgac cagcagcacc acacagagcg
    47281 cgagcaagag ggcggcgatt ttgcgatctt cgctctcctg ttcgcgcagc gtgctcatcg
    47341 cgtggcggac gtattggggt ccaaacactg cggcaagcga actggagagc cactgcaaaa
    47401 gtccacccat cacgagcgcg ggcacaccga cgagaccaat cgagcagagc agtgcttttg
    47461 cgagtgcatc gcctggaacc ggggtcgatg aagaagatgc ggccaaaagg acatctccgc
    47521 tgccgaccaa aaccgcagcg ataagtgcag ccgaaaagaa ggccagcggt cttggattcg
    47581 cgacgctggc aggggatggg gaggatgtgc tctcagttgc catggaagtc tctgaaacta
    47641 cgtgaagcag cgggcctgcg caatcgaggt gcacgcttcc catagatagt acttgcgaat
    47701 tatcagacca aacggtagcc tcgcggtggt aaagctagtt cggttcgtgg tccgtgaaaa
    47761 aggttctgca acatttggcg ctggatctcc tgtcgatgtc tcgactgcct gtgccgcctt
    47821 gggaacgtcg acaccgctag acttgttacc ggttcgaact gcaacatcga aggagagtca
    47881 gaatgggcac caaactgtat gtcggcaacc tctcgtacag caccacgagt gagcagcttg
    47941 gcgagatgtt ccacgctttc ggcgcggtcc gcagcgctca ggtcatcgaa gatcggatga
    48001 ccggacgcag caagggcttc ggcttcgtcg agatggagac cgacgcagag gctcaggccg
    48061 caatcaacgg tctaaatggc caggtcaagg gtggtcgccc gctgaccgtg aatgaagcgc
    48121 gtcctcgtga agagggtggc cgtggcggcg gcggtggccg tcccggtggc ggtggattcg
    48181 gtggcgagcg tcgcggtggc ggcggtcgtg gtccgcgtcc gtcgtactag ttcatccgtt
    48241 tcgtactgac gctcgcgtca gacgaacaaa tgcttggttt tttcctccgc atgtcggggg
    48301 aaggggccaa gcattttttt tgcttttggg gactggagct ggccggcagg actcgtcagc
    48361 gtgcttgtcc tgtcgttgcg cttattcgca gcggacggat tccatcatct tgtggatgat
    48421 ctcggcggcg ctctgcgggg ttttttcgtt caagatcgat ccaaaccggc ggagcacgac
    48481 gacttggtgc ggcctgactt ccttggccgc ttccatcaga taaaagtgac ggtgcatgcg
    48541 ctcgaagcgc tctttcgctt tgcgaaactc gagctgctgg tctttgagct tttcggtcgc
    48601 ttgccgctcg gcgcgacgga acgcatcgcg ttcggactgg ctgcggaagt catcaatgcc
    48661 gacccagatc catgggctta gggtgttgga cggaatccca aacgtgggca gctccgcgat
    48721 gatgtcaccg ggacgtagct cgctgcgcgg aacttgtcgt gcgttgacct gtccgacgcc
    48781 gtgtggcgat ggaatgtacg aaagccgcac ctgccagccg tctttgcttc cttcgcttaa
    48841 agtctgctgc ttgtctttga gcgcgtcgat gtcacttggg atcgcatagt cgaacaggaa
    48901 cgccagccgc ccgtgcacaa gccgatgggt gacgtcggcg aaacactcct tgatgaaggc
    48961 ttcggctgtg tcaccggggg gcagatcgta aaactccgcc cagacttctc cgtcggtggc
    49021 atcgttgcaa gtggccagct cggcttggcg accacggaac gtgaatggat cgtaactttt
    49081 gatccgaccg gcagatccgg acggaaactg cacagtgcag cggaatgtcg acgccgtata
    49141 gtctttttgg ttgcgcagct tttcgtaacg agcgcgctgc gcgtctgctt cggcgcgggt
    49201 ctttgaacgc tgcaccagca cgaacatcac gactgccacc gccacagcga ttccaatcca
    49261 cagcgtcatc gatttgcctt gcgtctttcc gttccgtgcg gagctggtca tgtcttctct
    49321 cctcgacgag cggagctaca gtttggccac agggttgggg cccggattta gcttgtgcca
    49381 agcgccaagt acgggcgtgg cagccacaca atccgctcgc cgatgccgac cagtccatcg
    49441 gtcgaaaccc cgagcgtgtc accttcttcg agcagctcga aacaagccca cggcacaccc
    49501 ggggaaagcg aagatgcgat gtcgaaatag ccgcgcggaa aagctgcggc ccgagggtga
    49561 ttggtcacaa aatcttctgg tacggcgacg tgaattcgga cggtgctgtg gcgcggattc
    49621 cagcttggcg caaagggctt ttgccacatc gcttcgtaaa atgtcttcgc ttggcgcgcc
    49681 gcgctttcga caaacacctg cgcatagtcg tcgatgctgg gccgcaggga acgcagaaaa
    49741 ggcagccgca gctcttcgcg tgagggatcg accagcacta caagtcgctg tttcagatca
    49801 tcggcaaaat cgaccagcag cgggggaggg gagtcgaggc gggcagacgg cgagccactc
    49861 atgggctcta tgttaatggc tcaggtagtc gaagacatcc tgatcggtgc ggccttcggg
    49921 cgcgtcgggg aaaaagtcat gctgcgaggt ctgggccaca atctgatcgc ggtgatcggg
    49981 cccttgcagc ggcgagtgat gttcgtttat ctcgcgctcg cgggcatcgg actgggcctg
    50041 ggcctgcttt tgctcttggc gttcttcccg tgccttttgc tgttccagct tctgctgctg
    50101 tcggcggagc gctttttggg tcgctttgtc gtactcgggc tcggtggctt ggccagtgcc
    50161 cgtcgcagcg ctttccgggg cggcctgtcc cgtcgtgcca cccgtcgctg cggtttcagg
    50221 cctgagcgga ccgtacagtc cttccaggcg ctcgcgcagg gccagttttt tgtcgaccag
    50281 ctcgccttcc ccttcgggcg cgtctttgcc ttgccagttt ttccacatcc gacgcagcgt
    50341 ttctttttcc agcttggctt ggtctgcgcg gtgttcggca tcgacgcccg cgacaccgcc
    50401 ttggacgcct ttcattccgc cggtcagcat gctgttgaag atggcaagcc ccttgtcttg
    50461 cttgccttcg tttgcgggcg taaatgcatc gaccgtgccg tcgagcagcc cgccgaacat
    50521 gccgcttatg ccgcccttgg caccgccgac tttgtacttg tcttccatcg tcaccaggct
    50581 agatccccag tccgatggct tggttccttc tttgatctgc tctgcaccga cgagcgaacg
    50641 aatcttgttt tcgaccagtc ggttggacat cacgccgtct ttgacaccgc cggcaacgtc
    50701 tttggcccac ttgccttcgt tgaacttgtc ttcgccgtgc tgcatcaagc tgttaaggaa
    50761 gttgccgccg ctccaggtgt tgccgtcctt gagcgtgtcc atcgcggtgt ccatcgcgta
    50821 gtttttcgcc gctttgccga cgccgccctt cagcccgttg aggatcgact cgcccatgct
    50881 gaagtctttg tcgttggccg ccggatcgcc aaagcccgga attttttgca tccacatcat
    50941 gccggggtgt tctttggcga agtctttgtt ggccagcgtc gacggcagcg aacccataag
    51001 tccaccgaac gcgcctttgc caaacccgag cagaccgtct ttggccatgt tgccaaacac
    51061 gtcgccggcg ccaccttccc agtttttgcg gttcgtcgct gccgatgcca tgccgcccat
    51121 gcctccctcg aggcctttgg acagcacgct catcttcatt ttttcgatcg cgctcagctc
    51181 gcccgcggct ttggtcgggt cgatcttcag cacgttgcgc atgaaggcgt ccattttggc
    51241 gccttgcatg agctgaccga tgcccgccgt cgccatgccg ccggccatgc taaagccggt
    51301 gtcgaccaga tcgctcttga gctcgtcgcc gccgtagccg ccgcctttga cgagcgcttt
    51361 ggttcccatc atcgccgcgc cgctgccacc agcgacgagt gccgcggcca ggattggaga
    51421 accagccccg gctgtcgcaa tcgtcagcgc cacacctgcc gtcacactgg cgaccgtgcc
    51481 cgccgcgttg gcgatcgagt cggttcgttc ttgtaccgct ttggcgtcga tcttgttcca
    51541 gtcggcaagc tggttgattt cagcaaactc ggcctgatcg acttttccgt cgcccgcttt
    51601 cgccttgtca tacgtcgagt tcagccgctg attttgccga tcgaggtctt tgcctgcttc
    51661 gccgccgaac gtgtcgacga agaaattgcg gatgccgttg ccgcgctggt gcttgtagcg
    51721 ttcattggcc tgcgcaacca tgtcctcggg ggtggttggc ttgcccttca ggctcatctg
    51781 aatttcgaaa ccggcgtcgc cgcccgttcg cgacgtcagc tccttttcca tgtctttgcc
    51841 ggtcagctct gcgtatttga ccttcagctt ttcgatgtcg tctttcgact tgccttcgag
    51901 gatcgaattg atcttcttat cgtcggtgaa catgccaaac aggccggtgt tcatcgacat
    51961 tttgatcgcc agctcgtcgg acactttgcc cgattcagcc agctgctgcg ctttggccag
    52021 gtcgtcgtct ttgagcgtgc ctttgagctt ggcgtcgagc gcgttgtagt tggcatcgcc
    52081 ctttttgtct gcgggcgtca tcgtcgcgtt gtatgcgtcg cgaatcgctc tgcattcctc
    52141 gggcgacttg ccttcaaact gtttgttcag ccgctctttg tcagggccac cgaggccgat
    52201 gaacgcaaac atgccctgcg gtttgcttgc cgcttcgatc tgatacgccg ccgcgacatc
    52261 cggttttcca tcgagaagcg cctgtgcggc ctttttgtct tcgtccttga gcttgtcgcc
    52321 gaccatcgcg cccaggtctt ggccgttgct gatctgtttg taggcgtcag aaacggcttt
    52381 gcgctcttct tcggttttgc acgacgccag cgtgtccaaa atcttgtttt tgtcggtgct
    52441 ggcgccaaac catccgctgt gcgtcgccat tttcaggtcg gctgccattt cggacgcttt
    52501 gtcgccggtg gcctgcgcct gaaccttctt tagttcgtcg ccgctgtagt atttttgcaa
    52561 atacccgtcg acgttaaagc cctgcttttg cagctcggcg cgcgtcgctg ggtccattcc
    52621 tttgaagatt ttttcgacgg cagctttgtc cgcatgctga ccgccaatcc cgaggaagtt
    52681 gtccttttcg gcattttgca gaccggcaat cgccgctttc gctttgctgg ccggatcttt
    52741 ggaatcctgc gaaatcagcg cttggtattc ggctttgtcc ttgccgctca gcgaaccacc
    52801 gagagagctg tcgaggtcgg tgccatagtg gctcttgtat tcggctttga tcgcggcgat
    52861 ttcttcgggc gtgcgaccgc gcaggatttg cagcgtgctc gccgggctgg caaaccagtg
    52921 accgtcggtc tctttgcgga tgtcagcggc aagctgcgaa atttctttcg gatcgggggc
    52981 tttgggcgct gccggttggg ctgcgggctg gccgttaggt gccgtcgcgt tttgcccggt
    53041 ctgtgccgtt gccggagctt ggttttgccc aggcccagtc gtcgtgggcg atggtcccgt
    53101 ttgtggcccg gctggcgttg gcgtggcggt gggggccggt gacggagctg ctccaggacc
    53161 tggttttccc gtgtccccga acatgcccgc aaacggattc catatggacc acttggggga
    53221 tgcggcaggg ctgggatttt gcgcaaccgg actggctgtg ggcaccggag gcggcgtcgc
    53281 actggcggcg ggcgtcggtg tcttggccgg cgcggcaaag ggttgtgggg caggggtttg
    53341 ggtttgcgcg ggacgtttgt cttgggctgg ctcgctcacg acaaacttat cggctgcagc
    53401 agtccccggt tgcggtcaga aaccctgcgg tctaatggtc gccgtaagcc ttttcgcccg
    53461 cagtgatggc gacggggatg tgatttttct gcgatggcat cgggcagtgg aaatagggcg
    53521 aatacgcgca gagcggattc cacgcctgat tgaagtcgag gatcgcggtg tcggacggat
    53581 cgagatccgc caacagaaaa cgtccggcgc cgtaacttcc cgacggcttg ccgctcgtct
    53641 tgtcacgaaa aatcagaaac agcgtctttc cacccggcgt ttccagatac gcttcgagcg
    53701 tgcagtcgcg ccgctgttcg ccatcgggca gtgaaaagtg gagatcaccg acggaaatca
    53761 gctcttgcgg ctcgccacgc gacgccagca ctttgaccgg atggggggcg gtgcgaaaga
    53821 gcttggcttc gacgacaaat gcttcatcgt cggggtaatc atgcagaccg ctgtatgtcc
    53881 gctttgcctc ggcttggtcg gaataaatcc caaagccagg gtcatcgagc gagcccgtca
    53941 gcaaaagctg ggtctggccg agcgttagca catcgccttt gcgcagcgtc gcgcggtttg
    54001 ccggctgccc attgtaaagc acggtttccg tcgcggaaaa cgtcacttcg ctgcccaagc
    54061 gggacacgac aagctggccg ccaaagccac tgcgcagact ggcaaaaagg tgcagcggcg
    54121 acgagacctc gagtcccaca ggatgttggc caatcggaat gtgctgatag tcgactcgcg
    54181 cgagcggact tagcgcgctg cgcatccggg catcttggcc gcgacggaag ttttggcgtt
    54241 tttcagcagc ggtacggagt ttttccggcg aaagggcggg cgacacgggg ctattttgac
    54301 tttggtttgg tttcgatgtg cagccaaaaa gcgcaagccc tgtaaccagc cgcgccatcc
    54361 acttgcgtcg agaaagccgc atggaaaggg tctattcgct cttgccttct tcggtgatgg
    54421 tcactttgac aatcgagttg accgggatgt agtaaagccg accgctgccg tggcgcagga
    54481 taagctgctg ctcggggacg tattcgaagt acgtgccaag gaggcgcgag ccggtgtcaa
    54541 ggtggaccaa aagctcgccg gtgagcggaa tcggcgacga gacccctggc gcaagcggtg
    54601 cggtgatgcg ctggatgtcg ctccacaaaa gtcgccgggt ttcgccgctg ccgagtcgaa
    54661 tcgtcgtgtg catgccctgg accgattcga ccaagtcacc gcgcagggtt ttgccgtcgc
    54721 gtagcaccac tgttcgttcc ggcgagccga catgggacgt atcgcctttt cgacgggcgg
    54781 gagagatccg cttggcttcg ttccaggtga tgcggcgaag gtttccatcg ccaagcttga
    54841 gcaccaggtg gctgccgact tcgtattcca tcacttcgcc gtgataggtg atgttgtcgc
    54901 gggtggtgac ggtgcgttcc attcctgatc cgacgagcgg cgtcgagggt gcgaccggcg
    54961 gcgctgcggc aggcggtggg ctcgatggtg gaacgctgct cggcggcagg cttgccgatg
    55021 ggggcgggct cgacggcgga cttgggctgc tcggcggtgg cgctgggctt ggcgacgggg
    55081 cagcagcggg cgctttggct ttcgggggcg agacttgatc gatctcatcc caggcgatgc
    55141 gcttgctttc gccgctcgac agcttcagcg tgatgtggga actgatgacg tactcgagca
    55201 cttcgccacg gtacatgctg ccgtctttgg tgatgacggt gcgctcgact gggccttggg
    55261 cggactgggc ccaagccggg ccgaccggca caaaagcaga ggccacggtc catgaaagca
    55321 ggccacacag ggccaacaca cgtcgctgca tcgcagaact cctcttttcc atcatagcaa
    55381 agccggatgc agcgccgcta ccgcgcgatc ggcttcccta tggcaaaaga ggcagacgca
    55441 ctttcggttt ggaaggacgg agcctggccc agactgcgcg cggcgaatac gttcgaagta
    55501 caccaccgag agcaaacgcg acaagctgcc agcgtgacat cagggaaaag cgacccagtt
    55561 cacagccggc acacggcttg gggaggactc cgcttgccag ctcctctgcc agtttctgtc
    55621 ttgccgccaa aatgctatcc atcccgtcgc tgccgatcac tcccaaccgc tgattttcgg
    55681 tgtctttcac catgcagcat gcggtggcga ttccttgatg gttcacatac agcccatccg
    55741 taagccacgg acagcgccgc ttgcctggct gaaaacccgc gagcagctcg tcgtaaaagc
    55801 tcttgattcc cagcttgtcc tttgccgctt ggatgttccg tagctggcgg tgcatccgga
    55861 aacggaccca caaatcatcg acttcggaat cggacagaac ctggcgctgc aatccttccg
    55921 gatagttctt ttgatagagc ggcatcgtcg atagcggctg cacggtgagt ccgccatcaa
    55981 gacccagttt tttgtaaagc gagagaatct gggaaaagtg atcttgggtg tcgcgcagca
    56041 cggtgacaga cagtccgaca agcggctttg gctgattctg tttccgtcgc gcttccatca
    56101 aattggacag accggcgatg actttttcca gcttgccacc gcgaatctga cggaaggtct
    56161 ctgggtcggc agattccatc gaaacagata ctttttcgat tcccaaagac accagctctt
    56221 cggcggtact ttcgctcatc agacttccgt ttgatatgag cgacactttg atgttttttg
    56281 cgcgcagcgc ccgcaccatg tcgaaaaatc gcggatgcag aagcgactct ccttcacctt
    56341 ggagttcgac atgttcgacg gaaggatgcg cggctaggag tgtctcaaat cgctcgaacg
    56401 gaaggtcggc ttgctccatg tagcgaccgc agcagaaacc gcaggtgaag ttacagcgtg
    56461 tggtcggttc aatctgtagg aagcgaacgg agtgcacttt gtgacgatag cagcagccag
    56521 caagctgtgg cgcaaatatc tatctgtgca gcggactccc ttccgtgcgg aaaaggaatc
    56581 gcttactgat actcggggga ttcgttgcga acgagcgggc gttcatcgct tgagcggttg
    56641 aaaccaaact gggtgttgta cgtcgtcttg tcgagtgagc agcgggggcc attgtttgcg
    56701 gcgagccggc tcggacagac acgctgaatg taggcgagat cggtttcgcc gtagatggtg
    56761 ttggcctgga gccagcgggt gtgacggatg caggacgccc cgtcggctcc ccactctgct
    56821 tccacttccc agccggggtt tgagctcttc tggatgccga tttggtcgta gacgttgatg
    56881 atggtgccgt tgcgggtatg tgagacgcca tcgccgcagt agtcggcacg gaccaagcgg
    56941 acacaggctc tgtgccagtc ggacagcttg cgcgtcttgc aggagttgtt gcggcattcc
    57001 tgcgcggtgg tccaaggctt atagccccag cgcacgcact tgccgattgc agcgtttacg
    57061 cagccaaagg taaagctgtc gtcttcatcg acgtagcttg ccgctttgtc ccaatatcca
    57121 gacacgacga ccgcggggat gggcacgccg tttgccgtac cgcaaagcga catcggacca
    57181 ttgtcggaca gcacggcgac cgaatagagg accgttccat cggcatcgat tttcacgtcg
    57241 tcgatgcgca ggggaacgct ggtgccgttt agcaacgtgc caccgagtct ggctccgacc
    57301 aggtcgctgc ctcggtaggt tcggtcttca gcccgtgcca ccagtgtcgc actgtcgatg
    57361 gctgcgtcct ccagcgtctg gttgcccagc gtgatgttgg aaaacgagac cttgcccaag
    57421 tcgaccaggc gatcgttgac cgaggcgagc cgtggtgcgg tcgtcgagct gctgttgagg
    57481 gtgagaccgt tgagggtgag accgttgagg gtgagaccgt tgagcgtcag accattgagc
    57541 gtcagaccgt
//
LOCUS       DAORGN010000007        55050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_189_length_55050_cov_6.055041, whole genome shotgun sequence.
ACCESSION   DAORGN010000007 DAORGN010000000
VERSION     DAORGN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 55050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55050
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_55050_cov_6.055041"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKL17_01595"
     CDS             <1..222
                     /locus_tag="PKL17_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HNK43446.1"
                     /translation="VPAANPAAVPKPVVPAAPVAAQPKPATAPAIPGLGTAKPATPVT
                     AAPTAAPRPVAPAPMPAAPRPANNPAQPQ"
     gene            233..1129
                     /gene="dapF"
                     /locus_tag="PKL17_01600"
     CDS             233..1129
                     /gene="dapF"
                     /locus_tag="PKL17_01600"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNK43447.1"
                     /translation="MPALPRRLPFVKMNGLGNDFLVVDLRKHPDWEPVLAQPLLVRRI
                     CDRHRGVGSDGILAVGPAQSAASVAAMRVHNADGSIAEMCGNGLRCVARYLFEQDEKR
                     FSQLDPPSLTVDTGAGALLCQFALSLDGVVQQVEIEMGQPKLLSPAPASLSVGKSELA
                     LTLVSMGNPHAVAFCDPALTYSQLLAQATEQGPLVEMHPLFPQRTNVEFVNVQGGAAH
                     LDVVVWERGCGVTQACGTGACAAVVAACLRGLVPLKTPCTVRLPGGLLTVTVAADYTS
                     VRMRGEAETVFCGEVITSSIMA"
     gene            1131..4799
                     /locus_tag="PKL17_01605"
     CDS             1131..4799
                     /locus_tag="PKL17_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43448.1"
                     /translation="MVPLSDGSAYPTLAPLGPDEKARFCGRARKVQRLRELLDARQIV
                     SLLGQCGVGKTSLVRAGLIAALRAEHDDSLAIVQIVHPGLDPFAELDAQGLVDSANDL
                     VLALRSIAQSGKRVLLVCDHLEELLFAEPYARQSFLAQLESLGAAIDGGLRVQLLLVA
                     NGDALSGLSVVAKGLGALLERQTMWLSPLLDVSEWTDMVRGPAREMGLRIEDGLIQAL
                     GKDIESYSAALQSGQRSGAVLPLLVAVMQRTEQVARLSPTPRVLSAEDYQSAGGLWRS
                     LPDIAESSWRSQPPALQFIQRRMLLLLLHRYHLAEGEVLFSRPRRRTELLALVSPDRG
                     EDSSAARDVEALSRLLASTLARETAGSDVIELASPILLSVWPELTRWPEDERRFEQWH
                     GELLAMASREGSLTGVGPVSVPMSQPAQPIGGHRLAEAERYLAERPAQIDLRVARLVK
                     TSLQAQVRRVPVPSPVPPRRRLLPWAMVGLLFLLLVGLLAERQRLQILSSQHADAIAA
                     EQGARAALLVQQPGQDGAALALAILSTAPALRNGRPVPTPAKEGLMLTYGTAKNSLPL
                     YGHTDRIELVAFDPTGELLLTGSLDQTARLWNARTGRPLRVLTDHGGLLTTAVFSPDG
                     RRVLTASTDGLGRLWEVETGELKVTLRGHTEPLEMGAFSASGDRIVTAGQDHTVRIWD
                     AATGVELRCLRGHSERVPMAAFVPSGNLVVSGSFDQTVRLWDISTGTELRTFRGHRHR
                     VNLVVVSSDGRRAATGSWDGTVRLWPIEAGSTLPPSGIEMMQGTPLHALQFSPDGGLL
                     ASAGADGVVNLWDGRSGEPRGTLHGHVGIVDALSFTSDGKYLVTAGHDRIVRLWDVAS
                     RLEIAVLRGHSGPIYGVAVALKGGKVATASHDRTARIWDVRSGQPLLVLSGHSRYIAA
                     SAFSPDGTRIATASHDHTAKLWKWPSGEPVATLIGHEHVIHGVAFSSDGMRIATASSD
                     GTVRVWDGQSGRRLALLKGHKGIVQTVVFAPDGHRLLSGGSDETLRLWDTERELLLRT
                     ESGHHGSLMQATFSADGRYVATVGSEGETVIRDGTTLAVVRVLPTSGQVVSSAIFLRT
                     PLGLSLLTAAERVVSKWDVETGQQKVLLSLPNPVQMAVLSPSGEHLLVVGTDNSLLLW
                     DMAAEMPLMRWPSMPFELSDANFAAPDGHYFLLSTADGKTLVYPDEYRSNLSGTLTDA
                     CNLLRYQPDFKAVASYCP"
     gene            complement(4842..7391)
                     /locus_tag="PKL17_01610"
     CDS             complement(4842..7391)
                     /locus_tag="PKL17_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HNK43449.1"
                     /translation="MSRTPERPGDKPDGSDSDDGAPGSTMSLRQGANPASDSGDVMSR
                     AAPPSKGKDRQSVDRESSDEKDPRIGQRFGKYKLTRLIGLGGMAQVYEAEDTLLNRRV
                     AVKFLTESRRQQSTAVERFINEAQVAGRLNHPNIIAIYDIGFEHDTYYIAMELLNPGS
                     AGSFIKQKGRMTWVEAAQTIVQCCGALDAAHKAGIIHRDIKPDNILCSPAGTAKLADF
                     GLVKELHIEDSAGLTQSGVVVGTPLFMSPEQCTAQPLDPRSDIYSLGCTFYALLTGSP
                     PFPTGSVPQIMLQHCKSKVPDPRVLAPDVPEAIVRVVERSTTKRPEDRYQTAAEMQAA
                     LTSALEESPRPTFEFLVLGAAARPSSRQRGSGAARLRSESGPRSPDSQGSPGGNKVTE
                     GTPTPNATPSADAPPPNPSIFSRRAALLGGIGLLAAATGVAYWRLRQPPASDNPGQNS
                     NQGAAKEKLRVGVLNSLSGPLFDSGRPVIDATLLAIEELNSRGGVLGRQIEAIVADGK
                     SDNGVFAREAERLLVEKQVITIFGGWSPSNRRTLKKIIEKHKSLLMFPARDEGMEDSE
                     YIVYSGSTPNQVVTPALRFFVEQGLFKRLFVIGTDGLSSNISAHLIRDELKVYPQISV
                     VGEQFALLGETGFKKAIEKIKESKPDLIFNFMIGVSNPAFFRELRAAGITAKQIPTVS
                     FAVGEPELQQTADLDMVGEYLAASHFHSLRTPQNQAFVSNIRRKYGDYRVTGASMDAA
                     YASVYLWAQAVQAAGTDDVARIRERLAGQAFEAPGGKTRIDPGNHHCYKPFYLGKITD
                     GSTVEIVKSITEPIRPEPFPATRTRAEWEALVEYLSKNWGGSFVNPGKPSG"
     gene            complement(7454..7837)
                     /locus_tag="PKL17_01615"
     CDS             complement(7454..7837)
                     /locus_tag="PKL17_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43450.1"
                     /translation="MRALWTTLLGLLFAGCSSVNTHPLKLATEVATPCNPVKEPGCRP
                     VYVSCEQLDLKELTLRVGVFDAESTTIQCPKELSAGPVTVDVQYRTGEPFYMIDTSFV
                     REGLTQNVSAGPFSDTDPDWRMMIR"
     gene            7943..8404
                     /locus_tag="PKL17_01620"
     CDS             7943..8404
                     /locus_tag="PKL17_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43451.1"
                     /translation="MQTQEPSPASPDKPWLPFRLVYLVLVLNFAIPALFYLFLPNFAV
                     DRFEEVGRTLGDGLYPMRAGELGFMWRVLAAGNVMTLAFMCGLILWDVRKYYIVLVPL
                     VFLKGFSALAYLGVFLIALPYRGFLAIFALDSVTAIAMVVFARRAYQLLSR"
     gene            8498..9922
                     /locus_tag="PKL17_01625"
     CDS             8498..9922
                     /locus_tag="PKL17_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43452.1"
                     /translation="MSFFSSIVEAPGHLMHGMGEMGEAAGSHAEGMMHAGGDVAGGIG
                     HGVADVAGGVMHGAQDYAHDVADGAHHAWGDLKQGHVGAAVGDMAGGAMHGMMHAGGD
                     ILHGLVDGVGHVGGGIMDGAKHIFQGEKGMLQHLIAGGKEMGKGFAPIKQGMGIFKDI
                     KGAWDLMHGKGSAEELKNPLLNAGEHGLKAIKELFGGEDKGKEGEGGHGKKEEKDGLD
                     KGLDMLKSGVGGVKSLVELPAKFSGLTNTIGKVITEGVPGAGMIEKLSGGLLKESMIA
                     EKAGAVLKPLHGFLEKGGEKILGSGLGSKLLEKVAVEGAEKGATSLATKFAGKIVPGL
                     NMATAAISAFQSGKSAYDAFKKGDVGGAVIEGFHTLTNIAGGLPIPGAGLISVGGDIA
                     AGAAHWLKDGGAKKIGEFASKAASSIGGAVSGAAKAIGDSPVGQFAKGAGKAIGGAVS
                     KVGGAVSNLASGALSGAKKIFSGW"
     gene            9941..10888
                     /locus_tag="PKL17_01630"
     CDS             9941..10888
                     /locus_tag="PKL17_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK43453.1"
                     /translation="MSLSSLPSVSTSSQPSRCLIGALALSALCLGSSACSSRPTIDPG
                     ATYIIGAGDIAYCGVKVATEAPAASTAALVAANPSAAVFTLGDNAYDSGTIDEYQNCY
                     GPTWGQFVDRTFPTPGNHEYVTPGADGYFSYFGDRAGPSRRGYYSYDIGTWHFVSLNS
                     NVDAVQGSEQETWLRADLTAHRGQRCILAYWHHPVFSSSSVHGNNPWMADIWRTLSEF
                     GTDVILTGHDHGYERFAPQRADATPDPDHGIREFVVGTGGANLYSFASPQPNSEVRID
                     TTFGVIRFGLKDGQYDWFFVPVDATAAFDSGQALCHPKP"
     gene            10989..11549
                     /locus_tag="PKL17_01635"
     CDS             10989..11549
                     /locus_tag="PKL17_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43454.1"
                     /translation="MAGKSLAETGVDVTSRFEQELQSRADDLYREAALAMLTKLHPSN
                     KVTVEEFLSALEQHKDVWSAVSRMGVIEFAMALGGRGGATEDESEESVSSGSAQGKRT
                     RLSESQKNSLKGIVISILGGARDGYSRTDLAAQISNDLLSTVGVVREELADKLRQPLH
                     ELVAENKIHTVGEKRLMRYHNGAKRS"
     gene            complement(11546..12685)
                     /locus_tag="PKL17_01640"
     CDS             complement(11546..12685)
                     /locus_tag="PKL17_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNK43455.1"
                     /translation="MFHTQPASNPPRRFSLSVHRPSDLDALPISAQERNRNHDAEHAF
                     NVRIPKHYLSSIDFGNPRDPVRLQTIPQAEELQFDPREISDPIGDQKHSPVPRLTHRY
                     PDRVLLYPTYQCAVYCRHCFRKESLSDAGDQFSATALEPAFGYIRNHPEVQEVIITGG
                     DPLVLSNQRIAFLREQLEAIPHLRMIRFHTRIPVVMPQRIDDGLVTALSGRLMVCIVT
                     HFNHPQEVVPACQVAAEKLRRAGFVLLNQTVLLRGINDDAETLKTLCRELFYTLGIKP
                     YYLHHCDLTHGLTHFRTTIEEGLSIMQKLRGHLSGVCLPQYVLDLPGGNGKIPLGPSY
                     VEDKAGYCWTFRNYQGKLCRYEEVVSENPPQQEGNQTNGEGNERR"
     gene            12863..13690
                     /locus_tag="PKL17_01645"
     CDS             12863..13690
                     /locus_tag="PKL17_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNK43456.1"
                     /translation="MSPQQIEQFLQKTLDSLAPLVLDIGGKILGAIALWIVGRVIIGA
                     VRRLVRRATEVRKVDATLIRYLDSVIGVMLQILLIVAILGVFGIQTTTFAGLLAAAGV
                     AIGMAWSGLLSNFAAGVFLILFSPFKVGDRISAAGIVGCVHELGLFVTSVDTDDGIRT
                     YVANSKLFSDNIVNFSANPYRRVDMGAQLAHGVNPVEAAARLKARLAQIPNVLKDPAP
                     MVEIDKFNMAGTVLLVRTYTHDTTFWPVYFAMTQTIADEFTKAGYPTPAEHKVQYNK"
     gene            13742..14695
                     /locus_tag="PKL17_01650"
     CDS             13742..14695
                     /locus_tag="PKL17_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43457.1"
                     /translation="MSTPSSPSLWQRFLLYTSPDSGTPIPDAAQDRFLFGLAQPVLGL
                     RLLASEQGFLIDSLKPALLLAGFCALAALLHLEIEETESVKHLGFLRRFYATFAVLAP
                     LPSILLSSHYAKLAAKAHRRLRFGDCTHRRTPLLRAAIHALKQALVIAISLAPLFGIL
                     SHFPWIGAKTVTLLGALWALHWVVVEALDSGRVDPVLAQQNREGTAVSSTENDAALGN
                     GAAKDEPPVVWFVRPMFRLADKLPIGKTPVRWFASFCRYLSRPWQEEIALTEQHPVLV
                     FGFALATAALLATPVLNLLFRPIIVTAAVALLGQLALRPGD"
     gene            complement(14745..16022)
                     /locus_tag="PKL17_01655"
     CDS             complement(14745..16022)
                     /locus_tag="PKL17_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43458.1"
                     /translation="MGCGSDGMDTTDNYIHPLPEGTKICGRPDFAEMFPYSIRSSTLP
                     VLVHYYRPQEEQTAKQALAYVEKGWDYHVNRLGMRQPLTDGGECGPDDSFDVFIWKGH
                     RSCLVNVLGEEPKTPWDDRRGFMVLDPWGPYGGSDLEETVVHEMAHGSQAADDWYESP
                     ITFEMSAVFTDQVYASRFIKTYFEDFQLHPDWALDRDDKYETWYMYGASMYLLYLKDR
                     YYDGDARFVSRMWLDSRNPPGAEKDQTLNEPDFVDALDGMLKTKAGVSYEDTVLEFSR
                     WRYYTGEHADDRHFRHFLYGQENLAAAKLALAAQQEAKPGKISIAADSAPMIHGSSYI
                     ELSKGTSTPATVMVALEAANDPSRRFVVQAVPGLSPGSDGEFLDLSTGPKPIQFAADG
                     KRTLIVTVMPTGPYDPDLRDETRHPFALSLTGM"
     gene            complement(16237..16896)
                     /locus_tag="PKL17_01660"
     CDS             complement(16237..16896)
                     /locus_tag="PKL17_01660"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNK43459.1"
                     /translation="MLKLCGFPISNYFNKVRLALLEKGVPHELDITAFPSQEAQFRQR
                     SPMGKIPFLQTEHGILCESQIICEYIEELHPETPLLPRDPFAKAKVRELTQVLELHVE
                     LVSRRLLAAAFFGGHVTDETKAEVKRDLDKGLRAFSQLVKFDPFLAGSEFTLADCAAA
                     NHFPLISMTTKRMFGSDALSELPQLADYLKLVSERPHVKTVYADRDAAFARVLNSRSG
                     S"
     gene            complement(16982..20290)
                     /locus_tag="PKL17_01665"
     CDS             complement(16982..20290)
                     /locus_tag="PKL17_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNK43460.1"
                     /translation="MIAALISLCARNRLLVVMLSVVACVVAGFCLKELPMDAIPDLSD
                     TQVIVYSRWEQSPDRIEDQVTYPIITSLLGAPKVKAIRGFSDFGYSYVYVLFEDGTDI
                     YWARSRVLEYLSKIKAKLPSDVKTELGPDATGVGWVFQYALVDESGQTPREELRSYQD
                     WFLRFAIQSVPGVAEVATVGGAIKQYQVTVDPNRLSAFGLSLSEVATALRKSNSEVGG
                     RLVEISGKEFMVRGRGYVRSIEDLEQVVLRTNDLGTPLRIADIGRVQLGPELRRGVAD
                     LDGKGEVVGGIVVMRMGENALSVIARVKERLSQLESSLPKGVRIVTTYDRSELILRAM
                     ATLKHDLMLEVVVVSLVILLFLWHIPSAIVPIVTIPVAVLLSFIPLYLLHVPANLMSL
                     SGIAISIGVLVDGAIVEVENAYRKIQKWQEHGSQGDFGLVRLQALLEVGPSVFFSLLV
                     IAVAFLPVFTLVDQEGRLFRPLALSKNLAMAIAALLAVTLDPAMRMMLSRVTPYSFSP
                     RPLAWLCNRMLVGTYHREENHPVSRLLFRVYDPVCRLVLQHPKTTILSAIFLTLSTVP
                     IYQKLGAEFMPPLHEGSILYMPTTLPGVSAAESLRILSLQDRILRSFPEVESVFGKAG
                     RAETSTDPAPLSMMETTVVLKPESAWRKKDQFYSGWPIPEAVKNALLRPLWPDHISHE
                     ELVQKLDDAMRIPGTTNAWTMPIKARIDMLSTGIRTPIGVKVYGQSPSELDRIGAQIE
                     SAIRSVSGTRSVFAERIAGGYFIDFALRRDALARYGLSVEDANQVIAQAIGGETVTTT
                     IEGRERYSVSLRYPRELRQDLAELSRVLVPLPSGNSVPMGQVADLKLSTGPSMLRDEN
                     GMLAAYVYVDFDTEQRDIASYVEDARRAVAHSVQLPSGYRIEWSGQFENMLRVRERLT
                     LIVPITILLIFLLLYANTKSVTKALIVLCAVPFSAVGAIWLLYLLGYHTSIAVWVGVI
                     ALLGLDAETGVFMLMYLDLSYDEAKQEDRLHSHADLIEAVVHGAVKRVRPKAMTVMAA
                     LLGLLPILFSQGTGADVMKRIAAPMVGGLVTSFLLELLVYPPIYLLWKQRTIRTAATV
                     ASAVPQAAGRTETE"
     gene            complement(20287..21708)
                     /locus_tag="PKL17_01670"
     CDS             complement(20287..21708)
                     /locus_tag="PKL17_01670"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK43461.1"
                     /translation="MIRASKLFLLCLVGILPSLVAIYLYRELSSVRKGIAKESSPSAK
                     AEVWTCPMHLQYTSDKPGPCPICGMDLVPQRSLSQKTTPSLNLDSVQQSMIGLQTVSA
                     KRAAFGRAIRTTGRIAYDETLVHHIHTKYEAYVEHLHANYVGKFVKKGEPIVALYSPE
                     LYSAEQEYLIARKAQAGLSSLHGGPNLVESAREKLLLWNLSPSDIAQLERSGRASRTF
                     NLYAPISGYVIAKTAVHGMRIKPEDSLFDIVDLSRVWVLADVYEYELPRIEKGQTATI
                     TLPYWPDRTWQGQLSYIFPSVDPKTRTIRVRIEVDNPRGDLKADMFADVEVQVVSRDA
                     LVIPDDAVIETGRRKLVFVKDQEGKLMQRTITTGERSGHLYEVRAGLSEGEVVAKGAS
                     FLLDSEARLQGGTSDEETDTKAAPTPASDDHSAHQDHSAHQDHSAHQDHSAHQDHSAH
                     QDHSAHQDHSAHQGHAAQKGNAP"
     gene            complement(21705..22955)
                     /locus_tag="PKL17_01675"
     CDS             complement(21705..22955)
                     /locus_tag="PKL17_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK43462.1"
                     /translation="MFRALFRTWLAGLSAWLLLPSEIRAEPPVLTLPGLLSEVEAHSP
                     LLHSRRAAEAAAKTHARQAASVDDPMLMVELWQAPTSIERLPLMVTLKQALPWPGKLV
                     ARAQVARFDEARAQADTQLQKQQLRLATVRAYALYLFSHRSLAVKEENQKILQVIVAS
                     VDGRYRVGRAELAELLDAQAALHEQNTELIELGHRSKQAESDILALLGETSPRSLGVP
                     VSIPELVPLPSLDSLLEQATQNRPELRMAQLATQQEEAKVTQARSEQAPDFALWGSYM
                     VPLRSDMERTFTVGIQTSIPSFSLFKTKAAQQEAESLRTQNKQQELQLRASVLSEVRA
                     SYTRCETVLRHLGLHHDDLIPLSERAVQTARAGYQSGRVPLSLLLSATQRLLQQRLDY
                     ERYLAELMLRRAELDFSVGQGVSQ"
     gene            complement(23013..23405)
                     /locus_tag="PKL17_01680"
     CDS             complement(23013..23405)
                     /locus_tag="PKL17_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43463.1"
                     /translation="MVPLFRRLIALLVCLHVWACFGLPTQVAYRCTMMAGTRIAAPCC
                     PTSIADTHEYATIHDHCCEELPKPTIDMSGPQPHKVAQQVAHLVAVISRSLATTHDLP
                     ARPQVTRFSSGIPPNFAPPRQGITVLQV"
     gene            complement(23559..23870)
                     /locus_tag="PKL17_01685"
     CDS             complement(23559..23870)
                     /locus_tag="PKL17_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNK43464.1"
                     /translation="MGTRLYVGNLPYGAKEKEIQDFFEESGSRSVTQVKIITDRETGR
                     PRGFAFVELSDAKMAESAIAELHGTQFGGRKIVVNQAHTPRNEGRSGGGGGGGGGRGP
                     R"
     gene            complement(24110..24730)
                     /locus_tag="PKL17_01690"
     CDS             complement(24110..24730)
                     /locus_tag="PKL17_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNK43465.1"
                     /translation="MASLWLVCTPGLVFAAPLPSPTELMTKIDAVQKDLRSLRADFVQ
                     HSRVKLFRQQVSSEGRFLYAKGTPARLRWEYLKPDPSTMLLVGEQATLKMGSRAPQVI
                     DTGKDPTLRAIFAQLKLWLGQGSLRDAEGEYEVKTGGTSDQPALILVPKPDSVLAKVF
                     SRIELHVDGKTMLLRRLLMVEQSGDEKEVIFSKLDRNVELNASQFQ"
     gene            complement(24750..25004)
                     /locus_tag="PKL17_01695"
     CDS             complement(24750..25004)
                     /locus_tag="PKL17_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNK43466.1"
                     /translation="MTQSSFVHEIKQLIISSLKLDKSPDEIEDNAPLFGGGLGLDSID
                     ALELAVAIERAYRVTIPDEKVGKQAFTSASALADYVATHR"
     gene            complement(25053..25805)
                     /locus_tag="PKL17_01700"
     CDS             complement(25053..25805)
                     /locus_tag="PKL17_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK43467.1"
                     /translation="MKLALVTGGTRGIGLAVARRLAQDGFAVTVTYAHDATQAEQTVA
                     AARAEGVELAALRCDASSVEDWQKISQPGGFLDGKNIAVLVHAAGFTRDKLAMTMPVR
                     DFDDVLGVHLRGGFLAAQAVTKGMIAARFGRILFICSPSAWLGRRGQTAYGAAKAGLF
                     GLTKSLVQELSRFQITCNCVSAGLVDTALTADIPEPVRKELLASIPLGRPGTPSEVAE
                     LVAFLAAKEAGYITGQWLAADGGLDAIREDSF"
     gene            complement(25818..27284)
                     /locus_tag="PKL17_01705"
     CDS             complement(25818..27284)
                     /locus_tag="PKL17_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNK43468.1"
                     /translation="MQPPIPHKTASPESRAPKQRPLDVALRVLLRVDEGAYATLALTG
                     ELSKQPLPETERALITELTYGSLRQVRRIDRALVAHAPRGLDKLDPTTRALLRLGAYQ
                     LLFMNAQPHFVVDAVVTTIKKRRGAGLAGFANAVLRKLHSTGEPPLPPYPPDGATQSQ
                     DKWCKLLALHHGLPDSIVAQFLAVLPDPREVDALLRALSQPASTWLRLNPLRGTRDEA
                     LSALRDELPEPPKPHALLSTAVELRSGHPFSGPGFAKGLYTAQDLAAQLTTQLLFAAT
                     EKGPLSLPAGAILDGCAGVGGKTCHVAALTQNQREIDAVDRLPRKLELLRDHAHRLGC
                     TRIRTICCDLLDDKAALRPAYAAVILDAPCSGSGVLRRHPEARLRPPNIDELVTLQSQ
                     LLDRAAGLVVPGGVLLYSVCSLFVPEGPLQIAAFLARHPDFVPLPPPPDDPLFFSGPS
                     PLCEKKAPFALRTLPHTHNADGFYAVRLLRTCTAQPTE"
     gene            complement(27285..28682)
                     /gene="fumC"
                     /locus_tag="PKL17_01710"
     CDS             complement(27285..28682)
                     /gene="fumC"
                     /locus_tag="PKL17_01710"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNK43469.1"
                     /translation="MSKSTRIEKDTFGDIEVPSDRYYGAQTQRSLQNFRIGGERFPRE
                     FIYALGIVKKAAARTNARLGKLDPKLLPAIEQAADEVIGGKLDDHFPLVVWQTGSGTQ
                     TNMNANEVIGNRASEILGGVLGSKKPVHPNDHVNMSQSTNDSFPTAMHVAVVDRVANG
                     LLPALVRLAEALEAKSRQFANIVKIGRTHLMDATPLTLGQTFSGYAAQVRGAHAAVKA
                     ALPQMYELAAGGTAVGTGLNSHPRYAIEVAAEIASLTGLPFVTAPNKFAALAAHDAVV
                     SLSGMLKTTAVALNKVANDIRLLGSGPRCAIGELKLPENEPGSSIMPGKVNPTQCEAM
                     TMVCAQVVGNDTAVAFAGAQGHFELNVFKPVLAYATLQSVRLLTDACHSFVENCVVGI
                     EADEVRIKELVDRSLMLVTALAPKLGYDMAAKIAKTAHKEGKTLKQVALQFGVLTEAE
                     FDNVVRPEEMCEPKP"
     gene            complement(28751..29953)
                     /locus_tag="PKL17_01715"
     CDS             complement(28751..29953)
                     /locus_tag="PKL17_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK43470.1"
                     /translation="MTPVESFFATNVVQFARLLRKAGLRIGPSSVLDALTALQVIDLG
                     SREQVRAALRAVLVHRSEELAVFEQAFALFFEPRRPLPHSDDPMPKKTPSPPGDPLLR
                     RLTDSLFGRQAAAPNAHDEPPVPDGVPSWSAEKALRQRDFADMTAEELAEARKLIAKL
                     DFALSPRPLRRTRPSPKGSRFDFRRTLLHTLRSHGESLLPHYRKPIQLPPPLCVLCDV
                     SGSMSRYSEMLLRFSHTLMAQRRRVEVFLLGTELSRVTQSMRGLDIDLALRRISLKVH
                     DLGGGTRLATALHDFNQHFSRRVLGQGAWVLLISDGLDRDEKYDLSKEAERLHKSTKR
                     FVFCNPLLGFSEFSPKAAGIRAILPHVDDFRPIHNLDSLADLVAALRQEPTPLQRRAV
                     SATVLAAK"
     gene            complement(29957..30865)
                     /locus_tag="PKL17_01720"
     CDS             complement(29957..30865)
                     /locus_tag="PKL17_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK43471.1"
                     /translation="MTEIPTSVDDLSQLLARQRYVADVGLSTVLFLALRLSRPLLIEG
                     EAGVGKTELAKVLAQALGRSLIRLQCYDGLELSQAAYEWDHARQLLAIRFAEAMGERD
                     REALSQELYSEKYLIRRPLLSALSPHPEGPPVLLIDEIDRADEPFDAYLLEVLSDFQI
                     SVPELGQIKAPSPPLCILTSNRTREIHDALKRRCLYHFLGYPSEKRELEIVSARAPQA
                     GPQLAAQVVALVQRLRQLDLFKPPGVAETLDWVAALISLSRRAVCADSVDATLGVVLK
                     YQDDIAKVKGPLAQKLVAEVKAQIPG"
     gene            complement(30870..31670)
                     /locus_tag="PKL17_01725"
     CDS             complement(30870..31670)
                     /locus_tag="PKL17_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNK43472.1"
                     /translation="MRDFQFHRPSTVAQAKQLLQSTEAGKLLGGGQSFLPVLKLDMAQ
                     PSDLISLAGVTELRGIRSDGQQVTIGAFTTHAEVAASLDVRAKIPGLAKLADGIGDPQ
                     VRNRGTLGGSLAHADPAADYPAAILGLSATINTDRRTIAADAFFTGLFETALEPDEII
                     VSVRFPIPERSAYGKFASHASRYAVVGVMVAKFASGVRVGVTGAGPSAFRWKDAEAAL
                     TARFSPDAISGLSYPSDELTSTPEASAEYRAHLIPVITRRAIQAALGG"
     gene            complement(31689..34076)
                     /locus_tag="PKL17_01730"
     CDS             complement(31689..34076)
                     /locus_tag="PKL17_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNK43473.1"
                     /translation="MSTTTQSSGSIGAAIPRREDRRFITGAGRYVDDIVLPNQAYAYI
                     VRSPHAHAQIRSISTESARSAPGVIAVFTGQDVVADKVGGIPCGWLVKNKDGSNMVEP
                     PHPMLVADCVRHVGDQVAVVIAESKADARAAAQLIEVDYDVLPAVATLQDAVSDGAPT
                     VWAQAKGNVCFDWDLGDKAAVKAAMKSAYKVVEIEVRNNRLIPNAIEPRAAIGQYDAA
                     SDGYTLYTTSQNPHLIRLLLGAFVLGIPEHRLRVVAPDVGGGFGSKIYHYAEEAIVTW
                     ASKKIGRPVKWTAERSESFMSDAHGRDTVMKAHLAVDKDGKFLGLCVHNVANMGAYLS
                     TFAPAIPTYLSATLLAGCYSTPAIYVEVKAVFTNTVPVDAYRGAGRPESAYLLERLVD
                     KAARELGIDRLEIRRRNFIRKDQFPYKTPVALEYDTGDYQATLDMALKAVDYQGYSAR
                     REASKKRGKLRGIGFSTYVEACGIAPSALVGQLGARAGLYEAATVRVHPTGSVSVFTG
                     THSHGQGHETTFAQLVADKLGVPYDKVEIVHGDTDKIPFGMGTYGSRSLSVGGSAIVK
                     AVDKIVAKGKKIAAHLLEASEADIEWNQGEFRVVGTNRKKSIGEIAFAAYVPHNYPID
                     KVEPGLEESAFYDPKNFTYPAGCHVCEVEVDPDTGVVEVVEMVAADDVGVIINPMIVD
                     GQMHGGLVQGIGQALYEEAQYDESGQLLTGSFLNYGMPRASDFPFFKVGNHVTACTHN
                     PVGAKGVGEVGAIGCPPAVINAILDALSPLGVTDISMPATPEKVWRAISAAQAAK"
     gene            complement(34073..34549)
                     /locus_tag="PKL17_01735"
     CDS             complement(34073..34549)
                     /locus_tag="PKL17_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK43474.1"
                     /translation="MIRVSLKVNSKQVAVDIEPRTLLVELLRDKLQMTGTHVGCDTSH
                     CGACVVHVDGKSVKSCTMLAVEADGCSVTTIEGLAQAGKLHPMQESFHKHHALQCGFC
                     TPGMIMSAVELVEKRPDADEHQTREWLEGNFCRCTGYHNIVKAILSCRDAMREKSA"
     gene            34750..35481
                     /locus_tag="PKL17_01740"
     CDS             34750..35481
                     /locus_tag="PKL17_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK43475.1"
                     /translation="MASLRISILAVPLFFVAPAFAEDAAPSALTTATMNPVVADAGWK
                     FASDKGGVSVWFRPFPASSVNEVRGEAVFDVAADALFAVLGDIDAYAEIMPPTSLSKR
                     LSDGTSARSYYIEIDPPVVSRRYYCMDVVLSRPRVDQFVSEWRMWPQGCSPRARSMVQ
                     MADNSGRWRLSSLGPLKTKVEFQAHMDPGGQIPTWMVNRTTVGQIANTFASLRRAAQS
                     PRYAAMTKPEPQGEPERPSVSSKTN"
     gene            complement(35497..37428)
                     /locus_tag="PKL17_01745"
     CDS             complement(35497..37428)
                     /locus_tag="PKL17_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43476.1"
                     /translation="MSGKRDDGDKPGDSQDEAISPSQPGFVSRSQPGVRRRGVTSGSY
                     LPVSGPRKLPVSGRPVSGKPTLSRPSTAKNVSRQRRRSRLAELAALPGKLPEKLGEGI
                     ERGINVTANALRDPVGTVQQAVAALPVPKGLVLGWAVAMLLPIVLYFVDLGAGSLSLL
                     DAEVALVVRKVLAGQLLWNEAFRSVPPPTGGPIGIVPFVLLSRVFGMSEATLRLVPVL
                     AALGAASCLLAIAIDVGIGRNTGGLAGLVLLAMPLTYELSHRVLPDMLVAVASSGAVA
                     LVSHSLHGHKFDRHILPMQKATEEPEPLPLRRLPMLFAALGIGLSGVLDVRAGLVALL
                     FGFFDCVISHRYLLRKRRVWLMWLGGTLLLLGASAIHGGALRTLFHRPAPGIFMQAFD
                     AVWRQGPSFYGRHVGQVVVVTTCFGLLLGSMRRASRPLLVWVMLATLLTWVGGDLAPP
                     RGLGLVLPPLALAAAVGLQSPVRWLGSLGGIITTLALAGVVLAVVEGDPVLHQSDTIK
                     TLVQSLRHAPPTARRCVVGMSNTVPSYYSRRQIEQFESVHALKQSIGEKELFSCLVPS
                     DMLPEMKTLLLKQPTAQPAAIKPQARADKTAKPTAKPIPAKPPELGSEPIGTVLTTTF
                     DIEEPPIDTKGPSLVLVAR"
     gene            complement(37445..39007)
                     /locus_tag="PKL17_01750"
     CDS             complement(37445..39007)
                     /locus_tag="PKL17_01750"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNK43477.1"
                     /translation="MTPLFVCLSLVLAAVVFAYLRKQRITEWNRLQEETKLRAAELVD
                     DARTQAEKQLTQARSDAAEQKLRKVQQAERDFQQRRETLKRGAQQVAAWEQTLQRTEQ
                     MLETRIASLNEREKAISLSQARQKAAQERRATLQQQASDLLQKQAGETFADAKARLIA
                     DWTEQARAAGADRVRQLEQTQNAPETAGRAKRLLEISSQRYQGHYLTERLLSTLPIPA
                     EILDRVFGDGADVMTVIGEVANIKLAISDDRTSVRLEGQDSFGREISRRTLARLIKQG
                     TRAEPEAVRRIATSLAEDLDRETVELGRRAFKELEIPRAHPDIVRLVGRLNYRTSYSQ
                     NQWKHAVESAFLCGMLAAELRLDMKIARRAALMHDIGKSLTHAIDGSHAVIGADYARR
                     LGETELVANAIGAHHLDEPFNSPYALIVAASDAMSGGRPGARHEQEGSHIQRIDDLER
                     LVHGAVGVERCYALQAGRELRIHVKEGQVDDLRAAQMASELAQRISDNLVFPGQIKVT
                     VIRELNVVSVAN"
     gene            39110..40741
                     /locus_tag="PKL17_01755"
     CDS             39110..40741
                     /locus_tag="PKL17_01755"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNK43478.1"
                     /translation="MNPKRSASKQTLYLFVTGGVVSSLGKGLASASIGALLEARGLKV
                     AIQKLDPYINVDPGTMNPLQHGEVFVTEDGAETDMDLGHYERFISARMTRRNNFTTGQ
                     VYESVIRKERKGEYLGATVQVIPHITDEIKGNIIACSEGLDVLIVEVGGTVGDIESLP
                     FLEAIRQLRHEVGPGNSVAMHLTLVPYIAAAGELKTKPTQHSVQKLREIGISPDMLLC
                     RCDRPIPEGQKNKIALFSNVPQDAVFTSQDVSTVYALPLHLHEQGLDDKLCELLNIRS
                     RAPALYEWQSIVERVIRPAHEVTIGIVGKYVDLVESYKSLHEALIHGGIAHNTKVRQR
                     YIDAEEVEQKGAEHFLSDVDAILVPGGFGKRGVEGKIAAVRYAREKRLPFFGICLGMQ
                     IAVIEYARNILGLAQANSLEFDPDAPHRVIDLMNEQRSVENLGGTMRLGAYLAKIAPG
                     TLAHRIYGTDQISERHRHRFEVNNAYRDQLAKAGLTLSALSPDGKLVEMVEIPDHPYF
                     VGCQFHPEFKSRPHSPHPLFREFVAAALVHKGKRN"
     gene            complement(40762..43311)
                     /locus_tag="PKL17_01760"
     CDS             complement(40762..43311)
                     /locus_tag="PKL17_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNK43479.1"
                     /translation="MCAKAARKHRPQIGKQVPRAQQIPQLLVAIGLMGIGCQTPSLTP
                     VRVSDGDSAVEFDPSSSSLRLVRGDETLLRFDPDAWQVATVEKLDDRSSFDPYWLEYG
                     DDVLSASEPADMIWHHVDHISIDSATNLQITLSLVLSDGQKARLEIAPSGPGRFAAQL
                     VPDVRSERPVAYIRLRPRTDASEGFYGLGGWADEVSHRGKVRPMQQEIDVLESKSNEI
                     RVPIPLLIGTRGWGLFVESRRPGVFAVASKQADLVETTFGTSDDSKNGLRFHLLTAAE
                     PSSVLSRYYEITGKPLLPATWALGPWIWRNENKNQAQLEDDLKKIRDLDLATSAIWID
                     RPYATTVNSFDFEAARFPDPTSMIAKVHAAGLRLALWHAPYLEKGSPDLSLAKQKGYF
                     PPSSGVLLNGWSEPIDFTNPAAKQFWQQRVGRYIEQGIEGFKLDYGEDVMVGLGAARS
                     NWHFSDGSDERTMHHSYQLRYHETYAELLPKSGGFLLCRTGRFGDQKNVSVIWPGDMD
                     ATFASHRQPWKDRDGKTYLSVGGLPATVVMGINLGVSGFPFFGADTGGFRHSPPDKEL
                     YIRWFQQTALSSVMQVGDSTSQTPWEFTPENGRDQETLDLYREYARLHLRLFPYEWTL
                     AQELAQTGRPIQRPVGLAYPRLGQHPSDEYLFGDDLLVAPVLVRGARSRDVLLPPGDW
                     VDFFSGEVFPGNNPRTITVAAPLSKLPLFARAGALLPLLHPAIDTLSPTTDPKVESFA
                     RDPGKLYVVAIFGGPASEFTVYDGTRIKLDPVGQHYSISPGSVFVKGQVITVYPLPTE
                     PKQVTAGNEVLMRAGTSPDLDMTASGWTWSNQKGGTLTVKLGEAQREFTWK"
     gene            43428..45629
                     /locus_tag="PKL17_01765"
     CDS             43428..45629
                     /locus_tag="PKL17_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43480.1"
                     /translation="MHKPLLTLLVGLSLVSACGAPTKIAQVHDANILPLRSVRLYETG
                     IGYFERAGTLGSRATVLPVPAGHIDDALKTLVVLSRDQKTRVFGLEFASSVSPGMGRA
                     LAGLPLSEDDPIDFLHLLRSCKGGDVSVRVGQQSINGRLIDIVEHGHDGPVSTAASES
                     KNDGKDQPSDGKSIRAQNPSDPQLLLLSQHGELLRLPLSRVTSVRPLDPSFAPRLRTA
                     LDATSQRSANAQRLIRLLSRDGGPVTIGYVAEAPLWRVSYRLVVSETGKGGVLQGWAL
                     IHNDTDEDWQNVQVHLVNGRPDSFLFPLAAPRYARRTLITPDNQLSTVPQLLGQSADQ
                     LWGDNVGDSFGVGGLGLSGMGAGGGGRGEGSIGLGSIGTIGHGSGVGTGGTGSSSLLQ
                     VGNLAATAKSTGVESGALFTYSLGEPLELRAHGSALVPFVQDAIEVETITFMPSPGEP
                     ARSGLRFTNSTRQTIPGGTLAVLGETGLLGESALPRLKPSERRFIEYGVELDVEVSRV
                     RQTQKEEPQRLTFSDDEFEEHFLRKRDQLLRIENRSGQTRQLYLALNLAANSQVQGAD
                     RLDFDHEKKSPVAIFELAPQSRAERPLTTMEGLVRRSDLRSLQAARLLQLSTTSTVPA
                     TERSIAAEAYARQKDVEDAAKEVDKVRGEIKKTEKDLERLREHLKALGKDSGAGPEGN
                     PLVKRILEAEDRLSASQKRLESLESDQDRRRDAVRKVLERLPKNKDKSTGG"
     gene            complement(45648..46331)
                     /locus_tag="PKL17_01770"
     CDS             complement(45648..46331)
                     /locus_tag="PKL17_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK43481.1"
                     /translation="MIQRASDKHAQSPDRFRALELLRDDGSADAIIGLLRRFSFVYDK
                     TIEDEQEKEWVYHELVAMGAKVLSPLRKYMRESETLGWALKVLEQVAKGDDFLKALEQ
                     LCEQNDNSYVRDPSKKTQLVHFMGEHRDPQIAKLLLPYLEDIDEGVRFKAVEALLHQA
                     QADVVAEPLCKLLLNKAEESRRIKIRIVEGMADAGLSVSDKTSVERVIDELGIDATVD
                     GKGRIARRS"
     gene            complement(46485..49262)
                     /locus_tag="PKL17_01775"
     CDS             complement(46485..49262)
                     /locus_tag="PKL17_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43482.1"
                     /translation="MADKFPRPFGRYTLLSPLAQGGMGALYLACVGDRGMERLCVIKT
                     VLPHLADPEYVNRFRDEAKVVVRLSHGNLVPVFDAGMVESELYLAMDYIDGKDLRAVW
                     NRCAKKGIAFPVDVAVHIARELARGLHYAHNFGNIKLVHRDVSPPNVLVSFNGEVKLT
                     DFGLASSTLKLEKTAAGIIYGKVSYMSPEQARGEIIDGRTDIYAAGIILWELLTGRQL
                     FPQQGGEGHELIDRVRNPHIDPPSLRAPRVPAMLDKIVLKSLAKNPDERYTSGEQFRA
                     DLTAFLASNRDYVATDSERVRTFMADLFDEDIERERRQRHDLLDNVHDRLQAVRRQEE
                     SEREAAEQKAQRRADILKEIEKPDGQDKGKLREGEIPSQVMEQLSAYDTGKMLGTVIE
                     RWKILRKIGEGGMGRVFEAEHTEIGRRVAIKILHPVYSRTPEVVARFRMEARAASRIG
                     HPNIIEVTDSGTTVDGSFYFVMELLEGTDLAERLKAEKRLPATDALSIGTQVAQALSA
                     AHLANIIHRDLKPENIFLIHRDGQSDFVKVLDFGIAKSLEDSKEQKLTTPGMAMGTPE
                     YMAPEQAAGKGADARADVYSLGAIMYEMLSGKAPIAGENMMDILIRKATEDPEDIASR
                     RPDVPAPMAQLVMRMLSRSVDARPQSMADAAAEMQKILGDIRAGVLLVPSRPPPSKSA
                     TDIQTAKPDPNRRRTMLAGGVALAGGLLGLAGALYWVFTAPVQKPHPDAGAVKLVTPP
                     VVDAGIKPAVATNPPVSPPDAGTPTKPPQAATETNPGKTPVPPKKDRDDGDGKPGKLG
                     NLTMPPFIDRNVALGKIEEGRLALNANRNEVGFNLCYAAAGNPQVRGLALECMGEAEF
                     GRGRFGEAIKWGKQALKNKATTTDLYLLLGKAYYRQKNCKEARFYYAKILNGADSSNP
                     AAQRGMELCKE"
     gene            complement(49762..51330)
                     /gene="dacB"
                     /locus_tag="PKL17_01780"
     CDS             complement(49762..51330)
                     /gene="dacB"
                     /locus_tag="PKL17_01780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNK43483.1"
                     /translation="MPAVGLLLASPSAQARSHPELAAVSPPIESIGATNFSSKVETLR
                     RRLHELSHGPLLRKLRVGMTVHDARTGDEVFALNSDSAFNPASNTKIFTTAAALSVLG
                     ADYRFRTTMWSPARSVSASSPDTLDGSLFLQGSGDPSLQPADIVDLVRDLSRRGIQHI
                     SGDIILDGEFRNDTDLEQAATAPAQGSGALLLNRDAYRVRVLASSVGRAAHVTIEPSS
                     PFFLLRSSVKTVAGKKTKLSVDSHRKDGQLVIEVRGRIGSRRGKATVKKRIPDAREWI
                     SATLSTALRDSGIDLRGNIRIGPPPPGPLRLVAEHRSEPLSEICRIVNKDSNNFVADV
                     VWKTLGSVRYGLPQSLEKGARAVTEWLVPLGFRPERVRLVNGSGLTYENRVRPIDVGK
                     LLLRLYHSLELGPEFMQSLAVGGIDGTIHYRFRGQSYGLVRGKTGTLNGVSVLSGYVG
                     SHPGVLVFTIFIEGFRAKRLQQVRQLQAQIVEVLLKHIRSADPSPLLPPSSTTRPGEE
                     PGEDDLNASDEEPL"
     gene            complement(51408..51893)
                     /locus_tag="PKL17_01785"
     CDS             complement(51408..51893)
                     /locus_tag="PKL17_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43484.1"
                     /translation="MSLAPHLICKSLGEAKFKEMFLSLSSAAMKASMMEVGLSATRQV
                     NQTSLKKRNEEWAKRLWSTVLADKPGCKVMMFEWLRQTKTDLLIAFLDAIEVPHQKGL
                     TDADFMKDVPDAKLLAAAKFLLAHPSFDQREVAAYLLFLDFSNETDKFSSLDLQKTLA
                     R"
     gene            complement(52039..52878)
                     /locus_tag="PKL17_01790"
     CDS             complement(52039..52878)
                     /locus_tag="PKL17_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNK43485.1"
                     /translation="MGSSGNNAAMLLFFHGLVGASLFAAPPPVASMVTVPGGPFVRGD
                     SEGEPDEKPQQRITVASFSIDRMEVTVAAYAACVAAKACPELPRRKTPDSPNLPVTHV
                     SWQEATAFCAWQGKRLPSETEWEKAARGTDGRRYPWGNDFSCQMGNFDNFSMDGRCAE
                     EGAPGKPVSVGSYPSGASPYGALDLAGNVWEWTADAYRKDAYKQAVATAVHGSPSEKL
                     RVLRGGGCCSIFGLPRTSDRLGLPLDYRDADIGFRCAKDGAADTQPASGHATPHSFPP
                     TRP"
     gene            52917..>53605
                     /gene="radC"
                     /locus_tag="PKL17_01795"
     CDS             52917..>53605
                     /gene="radC"
                     /locus_tag="PKL17_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNK43486.1"
                     /translation="MSRAEFLARPEAARPRERLLFLGAEQLSDEELLAIVLGSGAAQP
                     LARRLLDETGGPQGLRRAGQSALCSLPGVGPARACQIKAALELGRRALAPEPLLGLHV
                     RSPSDIASVLSVELGQAEQEAVHVFGLDARHRIRCRHVAAVGQVDRVVVSVADVFRPL
                     IREGMAAAIVAHNHPSGVSTPSEEDQTLTLRLAQTGTMLSIPLLDHVIVAKSGYYSFA
                     DHGLLRSPLALP"
     assembly_gap    53606..53705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            53825..54490
                     /gene="udk"
                     /locus_tag="PKL17_01800"
     CDS             53825..54490
                     /gene="udk"
                     /locus_tag="PKL17_01800"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNK43487.1"
                     /translation="MNPLVIGVAGGTGSGKSTVAAKIVEGLPSQSVVILDHDSYYRDR
                     SDLTPAMAEQLNYDHPDSLETDLLVDQLRRLKSGQAIDMPQYDFKTHSRLKTRRRVEP
                     APVIVVEGILLFVEPDLRALLDIKLFVDTDADIRILRRIRRDLEHRGRTFQSIREQYY
                     KTVRPMHLQFVEPSKRWADIIIPEGGENRVALDLVIGRLQHYLSDHVHPQHPSEENRV
                     AHC"
     gene            complement(54563..>55050)
                     /locus_tag="PKL17_01805"
     CDS             complement(54563..>55050)
                     /locus_tag="PKL17_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNK43488.1"
                     /translation="IKQAVDWCRDELSTQDLDFIRSFQTDLDIKLDSQTQLRLFHGSP
                     RSHMENILATTPVEELEQMLGERRTTVMAGGHTHLQMLRQHRGTLLVNPGSVGMPFRE
                     FAFGQAPTVLAHAEYAMVEGKDGNVTVTLHRVALDRAALRTATAATNHPLKMPLLASY
                     R"
BASE COUNT        10480 a        16299 c        16780 g        11391 t
ORIGIN      
        1 gtgcctgcgg ccaatcccgc ggctgtgccg aaaccggtcg tgccggctgc tcctgtcgcg
       61 gcgcaaccga aaccagccac tgcgccagcg ataccaggtc tggggacggc caagccggcg
      121 acgcctgtga cggcagctcc gaccgcggct ccgcgtccgg tggctcccgc gccgatgccc
      181 gctgcgcccc gtcctgccaa caatcctgcg cagccgcagt agcgatcctg ccgtgccggc
      241 acttccccgt cggctgccgt tcgtgaagat gaacggcctt ggcaacgact tcctcgtcgt
      301 tgatctccga aaacatcccg attgggaacc tgtcctggcc cagcctctgc tggtccgtcg
      361 catctgcgat cgccatcgcg gcgtcggatc ggatggaatc ttggctgtgg gaccggcgca
      421 gtcggcagca tccgtcgctg ccatgcgtgt tcacaacgcc gacggaagca tcgccgagat
      481 gtgcggcaat ggcctgcgct gcgtcgcccg ttatctgttc gaacaggatg aaaagcggtt
      541 ttcccagctt gatccaccgt cgctgacagt tgacaccggg gctggcgctc tgctctgtca
      601 gtttgcgctg tctttggatg gcgtcgtgca acaggtggaa atcgagatgg ggcaaccgaa
      661 gctgctttcg ccggctcccg cttcactttc cgtcggcaag tccgagcttg cgctcacgct
      721 tgtgtcgatg ggaaatcccc atgcggttgc gttttgcgac ccggccctga cgtattcaca
      781 gcttttggcg caggcaacgg aacaaggacc gctcgtggag atgcacccac tttttcccca
      841 gcgcaccaac gtcgagtttg tgaacgtgca gggtggggcg gcgcacctgg atgttgtggt
      901 gtgggaacgg ggctgcggcg tgacccaggc gtgtggaacg ggggcgtgtg ctgcggtggt
      961 ggcggcttgt ttgcgtggac ttgtgccgct caagacgcca tgcaccgtgc gtctgccggg
     1021 ggggcttctg acggtgacgg ttgcagcaga ttacacgtcg gttcgcatgc gcggtgaagc
     1081 cgagacagta ttctgtggcg aagtcatcac atcgtcgata atggcctaag atggtgccac
     1141 tcagtgatgg ttcggcatat ccgacgcttg cgccgcttgg gcccgatgaa aaagcccgat
     1201 tttgtggtcg cgcgcgcaaa gtgcagcgac tgcgtgagct gctcgatgcc aggcagattg
     1261 tgtcgctgct cgggcagtgt ggggtcggca agacgtcgct tgtgcgggcc ggcctgattg
     1321 cggcgctgcg ggccgagcac gacgacagct tggcgattgt ccagatcgtc catccagggc
     1381 ttgatccgtt tgctgagctt gacgcgcaag gtctcgttga ttcggcgaat gacttggtgc
     1441 tcgcgttgcg cagcattgcg cagtctggca agcgggtgct gctcgtctgc gatcaccttg
     1501 aggagctgct ttttgccgag ccttacgcgc ggcagagctt cctggcgcag ctggagtcgc
     1561 tcggggctgc catcgacggc ggacttcgtg ttcagctact gctcgtcgca aacggagacg
     1621 ctctttctgg actgtcggtg gtggccaagg gacttggggc gctgctcgaa cggcagacga
     1681 tgtggctgtc accgctgctt gacgtcagcg agtggacgga catggtgcgc gggccggcgc
     1741 gggagatggg cctgcgcatc gaagacggct tgattcaagc gctgggcaaa gacatcgagt
     1801 cgtattcggc ggcgcttcag agcggccagc gcagtggcgc ggtgctgcct ctgcttgttg
     1861 cggtgatgca gcgaaccgag caggtggcga gactgagtcc aacgccgcgg gtgctgtccg
     1921 ccgaagacta tcagtccgcg ggcggtctgt ggcgatcact gcccgacatc gcggagtcga
     1981 gctggcgcag tcagccgccg gcgctccagt tcattcagcg gcgcatgctg cttttgctgc
     2041 tgcatcgcta ccacctggcc gaaggtgaag ttctgttttc gcgtcctcgt cgacgcaccg
     2101 agcttttggc gctggtcagt cccgatcggg gcgaagacag cagcgcggcc cgtgatgtcg
     2161 aagcgctgag tcgattgctc gcatcgacgc tggcccgcga aacggcaggc agcgatgtga
     2221 tcgagctggc cagtccgatc ttgttgtcgg tgtggccgga acttacccgc tggcccgaag
     2281 acgagcgtcg gtttgagcag tggcacggtg agcttttggc gatggcatcg cgcgaaggga
     2341 gcctgaccgg agttggtccc gtgagcgtgc cgatgagcca acccgcgcag ccgatagggg
     2401 gtcatcggct tgccgaagct gagcgctatc tggccgagcg accagcgcag atcgatctgc
     2461 gcgtggcccg tctggtcaaa accagtcttc aggcgcaggt ccgtcgcgta cccgtgccat
     2521 cgccagttcc gccgcggcgt cgacttctgc cgtgggcgat ggttggtctt ttgtttttgc
     2581 tcctggttgg cttgctggcg gagcggcagc ggcttcagat tttgtcgtcg cagcacgccg
     2641 acgcgatcgc agcggagcaa ggggctcgcg ccgcgctttt ggtgcagcag ccaggccaag
     2701 acggagccgc actggcgctt gcgatcttgt cgactgcgcc ggcgctgcgc aacgggcggc
     2761 ctgttccgac gccggccaaa gaagggctga tgctgacata cgggacggcg aaaaactcgc
     2821 tgccgctgta tggacacacc gatcggatcg agctggtcgc gtttgatccg acgggtgagc
     2881 tgcttctgac cgggagcttg gatcagacgg cgcggctgtg gaatgcgcgg accgggcgac
     2941 cgctgcgggt gctgacggac cacggcgggc ttttgacgac ggcggtgttt tcacccgacg
     3001 gtcgacgggt gttgacagcg agcaccgacg ggctgggtcg gctttgggaa gtggaaaccg
     3061 gggaactgaa agtgacgctg cgcggccaca ccgagccgct cgagatgggc gctttttcgg
     3121 cgagcgggga tcggattgtc acggcgggtc aagatcacac ggttcggatc tgggacgcag
     3181 cgacgggcgt ggagctgcgc tgtcttcgtg gccacagcga gcgcgtgccg atggctgcgt
     3241 ttgtgccgtc gggaaacttg gtggtttcgg gaagcttcga tcagacggtg cggctgtggg
     3301 atatttcgac ggggacggag ctacggacgt ttcgcggcca tcgtcatcga gtaaacctcg
     3361 tggtggtttc atccgacgga cggcgagcgg ccacgggaag ctgggatggc acggtgcggc
     3421 tgtggccgat cgaagcggga tcgacgctgc cgccaagtgg catcgagatg atgcaaggca
     3481 cgccgctgca cgcgctgcag ttttctcccg acggaggact gttggcgagt gcgggggccg
     3541 acggggtggt caacctgtgg gacgggcgat ctggcgagcc gcgtggtacg ctccacggtc
     3601 acgttgggat cgtcgacgca ctgagcttta ccagcgacgg caaatatctg gtgacggcag
     3661 gccatgatcg gatcgtgcgg ctgtgggacg tggcaagtcg gctcgaaatc gcggtgctgc
     3721 gcgggcattc ggggccgatt tacggcgttg cggtggcgct caaaggggga aaggtcgcga
     3781 cggcaagcca cgaccggacg gcgcgaattt gggacgtgcg atcgggtcag ccgctgctgg
     3841 tgctgtcggg gcacagtcga tacatcgccg cgtcggcatt ttccccggat gggacgcgga
     3901 ttgcgacggc gagtcatgac cataccgcca agctgtggaa gtggccgagc ggcgaaccgg
     3961 tggcgacgct gattgggcat gaacatgtca tccacggcgt ggcattttcg tcggatggga
     4021 tgcggattgc gaccgcgagt tccgacggca cggtgcgggt ctgggacggg cagtcgggac
     4081 gccggctcgc gctcttgaaa gggcacaaag ggatcgttca gacggtggtg tttgcgccgg
     4141 acggtcatcg cttgctgagc ggtggttccg acgaaacact gcggctgtgg gacaccgagc
     4201 gcgagctgct tttgcggaca gaatcgggcc atcacggcag tttgatgcag gcgacgtttt
     4261 cggcggacgg tcgttatgtg gccaccgtcg gttcagaagg cgaaaccgtg atccgcgatg
     4321 gcacgacgct ggcggttgtc cgcgtgcttc cgacgagcgg acaggttgta agctccgcga
     4381 tctttttgcg cacgccgctc ggactgagcc tgctgacggc ggcggagcgc gtggtcagca
     4441 agtgggatgt cgaaactgga caacagaaag tgctgctctc gcttcccaat ccggtgcaga
     4501 tggcggtgct gtcgccaagc ggtgagcatc tgctcgtcgt cgggacggac aactcgcttt
     4561 tgctgtggga catggcggcg gaaatgccgc tgatgcgctg gccgagcatg ccgttcgagc
     4621 tgtccgacgc caactttgcc gcgcccgacg gtcactattt tttgctgagt actgctgacg
     4681 ggaagacgct ggtgtatccg gacgaatacc gcagcaattt gtcgggaacg ctgaccgatg
     4741 cgtgcaatct cttgcgctac cagcccgact tcaaggcggt cgcgagctac tgtccgtaaa
     4801 cagcggctgc cgacgagctc gcagcgatct tttgcggttt tttagcccga cggtttgccg
     4861 gggtttacaa acgatccgcc ccagtttttc gacagatact cgacgagcgc ttcccactcg
     4921 gcccgggttc gggtggccgg gaacggctcg ggacggattg gctcggtgat gctcttgacg
     4981 atctcgacgg tgctgccgtc ggtgatcttg ccaagataaa acggcttata gcagtggtgg
     5041 ttgcccggat cgatgcgggt tttgccaccg ggagcctcga aggcttgacc ggccaggcgc
     5101 tcgcggattc gggcgacatc atcggtgcct gctgcttgga ccgcttgcgc ccacagatag
     5161 acgctggcgt atgcggcatc catcgacgca cccgtgacgc gataatcccc atatttgcga
     5221 cggatgttcg atacgaatgc ctggttttgc ggcgtcctca gcgaatggaa gtggctcgcc
     5281 gcgaggtatt ccccaaccat gtccagatcg gcggtctgct gaagttcggg ttcaccgacg
     5341 gcaaacgaga cagtcggaat ctgtttggcg gtaattccag cggcgcgcag ctcgcggaaa
     5401 aaggccggat tcgagacgcc gatcatgaag ttgaagatca gatcgggctt gctctctttg
     5461 atcttctcga tcgctttttt aaatccggtt tcaccgagca gggcgaactg ttctccgacg
     5521 acgctgatct gtggatagac tttcagctcg tcgcgaatca gatgcgccga aatgttcgaa
     5581 gacaggccgt cggtgccgat cacaaacagt cgcttaaaca gcccttgctc gacgaagaaa
     5641 cgcagtgccg gtgtgaccac ctgattcggc gtactgccgg agtagacgat gtactcggaa
     5701 tcttccatgc cctcgtcgcg ggcggggaac atcagcaggc tcttgtgctt ttcgatgatc
     5761 tttttgagcg tgcgacggtt cgacggggac cagccaccga agatggtgat cacctgtttt
     5821 tcgacgagca atcgttctgc ttcgcgcgcg aagactccgt tgtcgctctt gccatcagcg
     5881 acgatcgctt cgatctgacg acccagcacg ccgccgcgac tgttcagttc ctcgatggca
     5941 agcagggttg cgtcgatgac cggacggcca ctatcgaaca gcggaccgga cagtgagttg
     6001 agcacaccga cgcggagttt ttctttggcg gctccctggt tggaattctg ccctggattg
     6061 tcgctggcag gtggttggcg cagacgccag taggcaacac ccgttgctgc agccaaaaga
     6121 ccaatgccgc caagcagggc tgcgcggcgg gaaaaaatgc tcggattcgg cggtggtgca
     6181 tctgccgacg gtgtcgcgtt gggtgtcggt gtgccttcgg tgactttgtt tccaccgggg
     6241 ctgccctgcg aatccggact gcgaggacca ctttcgctgc gcagacgggc tgctcccgat
     6301 ccacgctgtc tcgacgacgg acgcgctgcg gcgcccagca ccaaaaactc gaacgtcgga
     6361 cgcggcgatt cttccagtgc cgaagtgagc gcagcttgca tctcagcggc agtctggtag
     6421 cgatcttcgg ggcgttttgt cgtcgaccgt tccaccacac gaacgatcgc ttccggcacg
     6481 tcgggggcca aaacccgcgg atccggcacc ttgcttttgc agtgctggag catgatctgc
     6541 ggcacgcttc ccgtcggaaa cggcggactt ccagtcagca gcgcgtaaaa cgtacagccg
     6601 agcgaataaa tgtcactgcg gggatcgaga ggctgcgccg tacactgctc ggggctcatg
     6661 aacagcggcg taccgacgac gacacccgac tgtgtgagcc cggcgctgtc ttcgatgtgc
     6721 agttctttga ccaggccgaa gtcggccagc ttcgcagttc ctgccggtga gcagaggatg
     6781 ttgtccggtt tgatatcgcg gtggatgatc ccggctttgt gcgcagcgtc gagcgcaccg
     6841 cagcactgca caatcgtctg cgcggcttcc acccaagtca tgcggccctt ctgtttgata
     6901 aaggagccgg cggagcctgg gtttagcagc tccatcgcga tgtaataggt gtcgtgctcg
     6961 aagccgatgt cgtaaatcgc gatgatgttg gggtggttca gtcggcccgc gacctgcgct
     7021 tcgttgatga agcgttcgac ggcggtggac tgctggcggc gcgattcggt gagaaacttc
     7081 acagcgacgc ggcggtttag cagcgtatct tccgcttcgt acacttgcgc catcccgcca
     7141 aggccaatca gccgcgtgag cttgtatttg ccaaagcgct gtccaatccg cggatctttt
     7201 tcatcgctcg attcgcgatc gacagactgg cgatctttgc ctttcgacgg gggcgcagcg
     7261 cgtgacatca cgtcaccgct gtcagatgca ggatttgccc cctggcgcaa cgacatcgtt
     7321 gagccaggtg caccgtcatc ggaatctgat ccgtccggct tgtcccctgg gcgctctggg
     7381 gttctgctca cggcaacatg atctgggact ttgcgcggtt aatcaagccc cacctcggga
     7441 tttcgaggcc agctcagcga atcatcatcc gccagtctgg atcggtgtcc gaaaacggac
     7501 ctgcacttac attctgtgtc agaccttcac ggacaaacga cgtatcgatc atgtagaacg
     7561 gctcgcctgt acggtactga acgtcgaccg tgacggggcc tgcgctcagc tctttgggac
     7621 actggatggt ggtggattcc gcgtcgaaca caccgacccg cagcgtaagt tcctttagat
     7681 cgagctgctc gcacgagaca tacaccggac gacagccggg ctctttgacg gggttacacg
     7741 gcgttgcgac ttcggtcgcg agcttgagcg gatgggtgtt taccgacgaa caaccagcga
     7801 aaagcagtcc cagcaaagtg gtccacagcg cgcgcatgcg cctttgtagc atgatttgtg
     7861 gacaggggaa gtcttgaagg ccggcttcca gcggcgtgac tccgacttgc ggcagccact
     7921 gccagcgggg tatgtgcaga gcatgcaaac ccaagaacct tcgcctgcca gccccgacaa
     7981 accctggctg ccatttcgct tggtttacct tgttttggtg ctgaattttg cgattccggc
     8041 gcttttttac ctgtttttgc ctaattttgc ggtcgatcgg ttcgaagaag tgggccggac
     8101 gttgggcgat ggactttatc caatgcgcgc cggcgagctg ggctttatgt ggcgggtttt
     8161 ggctgcaggc aatgtgatga cgctggcgtt catgtgtggg ctgatcctct gggatgttcg
     8221 caagtactac atcgtgctgg tgccgctggt gtttctaaaa ggtttcagtg cactggctta
     8281 ccttggcgta ttcctgatcg cgctgccgta tcgtggtttt ttggcgatct tcgccctgga
     8341 tagtgtcacc gccattgcga tggtggtgtt tgctcgccgc gcgtatcagc ttctttcgcg
     8401 ctagccatcg cgccgttgcc gtcgctaaaa aacgctggaa actttgtaat tccccttccg
     8461 ataatcgagg tagttcgatt ttgtaagggg aatttacatg tcgttctttt cttcgatcgt
     8521 cgaggctccc ggccacctca tgcacggcat gggcgagatg ggcgaagccg ccggcagcca
     8581 tgcggaaggt atgatgcacg cgggtggcga cgttgctggc ggcatcggtc acggtgtggc
     8641 cgacgttgcg ggcggcgtga tgcacggtgc ccaagactat gcacacgatg ttgctgacgg
     8701 tgcccatcac gcatggggcg atctgaagca gggtcatgtt ggcgctgccg tcggtgatat
     8761 ggccggtggc gcgatgcacg gcatgatgca cgcgggtggc gacatccttc acgggctggt
     8821 cgacggtgtc ggccacgtcg gtggcggcat catggatggg gccaagcaca tcttccaagg
     8881 cgagaagggg atgcttcagc acctgatcgc cggcggcaaa gagatgggca aaggctttgc
     8941 gccgatcaag cagggcatgg ggatcttcaa ggacataaag ggtgcttggg atctgatgca
     9001 cggcaaaggc agcgccgagg agctgaagaa tccgctgctg aatgcgggcg agcacgggct
     9061 caaggcgatc aaggagctgt tcggcggcga agacaagggc aaggaaggcg agggcggcca
     9121 cggcaaaaag gaagaaaagg acggtctcga caagggcctc gatatgctca agtcgggcgt
     9181 cggcggcgtc aaatcgctcg tcgagctgcc ggccaagttt tccggcctga ccaacacgat
     9241 cggcaaagtc atcaccgaag gcgtccccgg cgccgggatg attgaaaagc tgtcgggcgg
     9301 tctgctcaaa gagtcgatga tcgcggaaaa ggccggcgca gtgctcaagc cgctgcatgg
     9361 cttcctcgaa aaaggcggcg aaaagatcct tggcagtggc ctgggatcga agctgctcga
     9421 aaaagtcgcc gtcgaaggtg cggaaaaagg cgccacgtcg ctcgcgacca agttcgccgg
     9481 caagattgtc cccggcctca acatggcgac ggcggcgatc tccgcgttcc agagcggcaa
     9541 gtcggcctac gatgcgttca agaagggcga cgtcggcggc gcggtcatcg aaggcttcca
     9601 cacgctgacc aacatcgccg gcggtctgcc gattcctggc gcgggtctca tcagcgttgg
     9661 tggcgacatc gccgcaggcg cagcgcactg gctcaaagac ggtggtgcca aaaagatcgg
     9721 cgagtttgcc agcaaagcgg cgtcgtcgat tggtggtgcg gtcagcggcg cggccaaagc
     9781 gatcggtgac agccccgtcg gacagtttgc caaaggcgct ggcaaagcca ttggtggcgc
     9841 ggtcagcaag gtcggcggtg ccgtctcgaa cctggcctct ggcgcactca gcggtgccaa
     9901 gaagatattc agcggctggt agtccccaag tggcccactc gtgagcctat cgtccctccc
     9961 ttcggtttcg acttcatccc agccttcgcg atgtctcatc ggcgcgcttg cactttcggc
    10021 gctgtgcttg ggcagcagcg cgtgcagcag tcgacccacg attgatcccg gcgcgacgta
    10081 catcatcgga gccggtgaca tcgcgtattg cggggtcaaa gtcgcgacgg aagcgccagc
    10141 cgccagcact gcagcccttg tggcggcaaa cccatcggca gccgtcttta cgctcggcga
    10201 caacgcttac gacagcggca ccatcgatga ataccagaac tgttatggac cgacgtgggg
    10261 tcagttcgtc gatcgcacct ttccgacgcc cggcaaccac gaatacgtca cgccaggggc
    10321 tgacgggtat ttcagctatt ttggcgatcg ggccggccca agccgccgcg ggtactattc
    10381 ctacgacatt ggcacttggc attttgtatc gctcaacagc aacgtcgacg cggtgcaggg
    10441 atcggagcag gaaacctggc tgcgtgccga tctgacggcc catcgtggcc agcgctgcat
    10501 cttggcgtac tggcatcatc cggtgttttc cagttcatcg gttcacggca acaacccgtg
    10561 gatggcggac atctggcgga cgctgtcgga gtttggcacc gacgtgatcc tgaccggtca
    10621 tgaccacggc tacgaacgat ttgcgccgca gcgggccgac gcgacgcccg atcccgacca
    10681 cggaattcgc gagtttgtgg tcggaaccgg cggagccaat ctgtattcct ttgcgtcacc
    10741 ccagccaaac agcgaagtcc gcatcgacac cacgttcggg gtgattcgct ttggtctcaa
    10801 agatgggcag tacgactggt tctttgtgcc ggtcgatgcg accgcggcct ttgattccgg
    10861 acaagccctg tgccatccca agccctagcg tggcgacgag aaacccgact ttccttgcac
    10921 aaaagttgcg tcttcttccc tggcgcacac actacgcggg tcattcgcta ggcaaaggaa
    10981 ccattgaaat ggctggaaag tcgcttgctg aaactggtgt cgacgtaact tctcgttttg
    11041 aacaagagct gcaatcgcgc gccgatgatc tgtatcgcga agccgcgttg gcgatgctga
    11101 cgaagcttca tccctcgaac aaagtgacgg tggaagagtt tctgtccgcg ctggagcagc
    11161 acaaggatgt ctggtcggcg gtgtcgcgga tgggcgtcat cgagtttgcg atggcgctgg
    11221 gtggccgtgg cggggcgacc gaagacgaat cggaagagtc cgtttcgtcg ggatcggcac
    11281 aaggcaagcg cacgcggctg agtgagtcgc agaagaactc gctcaaggga atcgtgattt
    11341 cgattcttgg cggcgcgcgt gatggctaca gccgcaccga tctggccgcg cagataagca
    11401 atgatcttct gtcgaccgtc ggcgtggttc gggaagaact ggccgacaag ctgcgccagc
    11461 cgctgcatga actggtcgcg gaaaacaaga tccacaccgt cggtgaaaag cgcctgatgc
    11521 gctatcacaa cggcgccaag cgcagctaac gtctttcgtt cccttctccg tttgtctgat
    11581 tcccttcctg ttgcggaggg ttttcagaca cgacctcttc atatctgcat agcttccctt
    11641 ggtagttgcg gaaggtccaa cagtagcccg ccttgtcctc gacgtaggag ggcccgagcg
    11701 gaatcttccc attccctccg ggcagatcca acacatactg cggcaagcag actccggaca
    11761 gatgaccgcg cagcttttgc atgattgaaa gtccctcttc aatcgtcgtg cgaaagtgcg
    11821 taagtccatg cgtcaggtcg cagtgatgca ggtaatacgg cttgattccc agcgtgtaaa
    11881 acagctcccg gcagagtgtc ttgagcgtct ctgcgtcgtc gttaatcccc cgcaaaagca
    11941 ccgtctggtt tagcaggaca aaacccgctc tgcggagctt ttcggcggcg acttggcacg
    12001 cgggaacaac ttcctgtgga tggttaaagt gcgtcacgat gcagaccatc aatcgacccg
    12061 acagcgccgt gaccagaccg tcgtcgattc gctgtggcat cacgaccgga atccgggtgt
    12121 gaaaacgaat catccgcagg tgtggaatcg cttcaagttg ctctcgcaga aatgcgatgc
    12181 gctggttcga taggacgagt ggatcacctc cggtgatgat cacttcctgc acttccggat
    12241 gattccgaat gtagccaaac gcgggctcaa gtgccgtcgc tgaaaactga tcaccggcat
    12301 cgctgagcga ttcctttcgg aagcagtgac ggcagtacac cgcgcactga tacgtcggat
    12361 aaaggagcac tcggtccgga tagcgatgcg tgagccttgg cacaggactg tgtttttggt
    12421 cgccaatcgg atcggagatt tcgcgtggat caaactgcaa ttcctcggcc tgcggaatgg
    12481 tctggagccg caccggatcg cgcggattgc cgaagtcgat ggaagacaga tagtgcttgg
    12541 gaattcgcac gttgaacgca tgttctgcgt cgtgattgcg attccgttct tgcgcagaaa
    12601 tcggcagcgc atccaggtca cttggcctgt gaacagagag cgaaaatcgc cgcggcggat
    12661 tcgatgcagg ctgcgtgtgg aacacgctgg ggtcagcagt ttgggagtgg gaatggctgc
    12721 gattacgatt ccgttcggag gacatgggtg cttgcgttcc tgggctacga gccatccgag
    12781 ttcgttgaca aggccgtcta gtatctgtca acctcttcct cggtagtggc caatccatca
    12841 tttgtcagta gagaggtact agatgtcacc acaacagatc gagcagttcc tgcaaaagac
    12901 ccttgattcg cttgcgcctc ttgtactcga catcggagga aagatcctcg gtgccatcgc
    12961 gctgtggatc gtgggccgtg tgatcattgg cgctgttcgc cgactggtgc gtcgcgccac
    13021 cgaagtacgg aaagtcgatg cgacgctgat tcgctatctc gattccgtga tcggggtgat
    13081 gctacagatt ctgttgatcg tcgccatctt gggcgtgttt gggattcaga ccacgacgtt
    13141 tgccggcctt ctggcggcgg cgggcgttgc catcggcatg gcgtggtcgg ggctattgtc
    13201 aaactttgcc gcgggcgtgt ttctgattct gttctctccg ttcaaagtcg gggatcgcat
    13261 ctctgccgcg ggaatcgtcg gctgtgttca cgaactgggt ctgtttgtga cttccgtcga
    13321 cacggatgat ggaatccgta cctacgtggc caacagcaag ctgttttctg acaacatcgt
    13381 caacttttcc gccaacccct atcgtcgcgt tgacatgggt gcgcagcttg cgcatggcgt
    13441 aaatcccgtc gaagccgctg cgcgtctgaa ggcgcgtctg gcccaaattc ccaatgtgct
    13501 caaagacccg gcgccgatgg ttgaaatcga caagttcaac atggccggaa cggttctgct
    13561 ggttcgcacc tacacccatg atacgacgtt ctggccggtg tatttcgcga tgacgcagac
    13621 catcgccgat gagtttacca aagctggcta tccgacgccc gccgagcaca aagttcagta
    13681 caacaagtaa gcgatccgca ttcccaaagc gcgagcggcg ctcttttttc ctacccaaga
    13741 catgtcgacg ccatccagtc cttcgctgtg gcagcgattc cttttgtaca caagtcccga
    13801 ttccggtacg ccgattccgg atgccgcaca ggaccgcttt ctgttcggtc tggcgcagcc
    13861 ggtgctgggc ttgcggctcc ttgcgagtga acagggattc ctgatcgatt ccctaaagcc
    13921 ggcgctgctg ctcgctggat tctgtgcgct ggcggcgctg ctgcacctgg aaatcgagga
    13981 aacagaatcg gtcaagcacc tgggttttct gcgccgtttc tatgcaacct ttgctgtcct
    14041 ggcgccgctg ccgtcgattc tgctctcctc tcactacgcg aagcttgcgg ccaaagcgca
    14101 tcgtcgcttg cgctttggtg actgcaccca ccgacggaca ccactattgc gagccgcgat
    14161 ccatgcacta aaacaagcgc tggtcatcgc catttcactg gcgccgctgt tcggaatcct
    14221 ttcgcatttt ccgtggatcg gagccaagac ggtgacgctt ttgggcgcgc tgtgggcgct
    14281 gcactgggtg gtggttgaag cgctggacag cggtcgtgtc gatccggttc tagcgcaaca
    14341 aaatcgggaa ggaacagcag tttcttccac ggaaaatgac gctgcattgg gaaacggcgc
    14401 ggcaaaagat gaaccccctg tggtgtggtt tgtgcgcccg atgtttcggc ttgcggacaa
    14461 gcttccgatc ggaaagacac ccgtgcgctg gtttgcgtcg ttttgccgct atctgtcacg
    14521 tccgtggcaa gaggagatcg cgctcacaga acagcatccg gtgctggtgt ttggatttgc
    14581 gctcgccacc gctgcactgc tcgcgacacc ggtgctgaac ttgctcttcc gtccgatcat
    14641 tgtgacggcg gctgttgcgc tgcttggtca gcttgcgctg cgccctggcg actgacattc
    14701 ccaagccaga gcgcgagcga ttcgccagcg ggtttgaaac gggactacat tccggtcaaa
    14761 gacagcgcaa acggatggcg tgtttcatcg cgcaaatccg gatcgtaagg accggtcggc
    14821 atcaccgtaa caatcaaggt ccgtttccca tctgcagcga actggatcgg ctttggtccc
    14881 gtcgacaaat cgagaaattc accatcggaa ccgggcgaaa gaccggggac ggcttggacg
    14941 acgaaacggc gcgacggatc gtttgctgct tccagcgcca ccatcacggt agcgggcgta
    15001 ctggttcctt tggatagctc gatgtagctt gatccgtgaa tcatgggcgc gctgtctgcc
    15061 gcgatgctga tcttgcctgg cttcgcttcc tgctgcgcgg ccagcgcgag cttggctgcg
    15121 gcgagatttt cctgtccgta cagaaaatgt cggaagtgac gatcatcggc atgctcgcct
    15181 gtgtagtagc gccagcgcga aaactccagc acggtgtctt cgtagcttac tccggccttg
    15241 gtcttgagca tcccatccag cgcatcgaca aagtccggtt cgtttagtgt ctggtctttt
    15301 tcggcgccag gcggattgcg actatcaagc cacatgcgcg acacaaagcg cgcatccccg
    15361 tcgtagtagc gatccttgag gtacagaagg tacatcgacg caccgtacat gtaccaagtc
    15421 tcatatttgt catcgcgatc gagcgcccag tcgggatgaa gctggaaatc ctcgaagtac
    15481 gtcttgatga atcggctggc atacacttga tcggtgaaca ccgcagacat ctcgaacgtg
    15541 ataggacttt cgtaccagtc gtcggcagct tgtgatccgt gcgccatttc gtgaacgacg
    15601 gtttcttcca gatcactgcc gccgtatgga ccccatggat caagaaccat aaaaccgcgc
    15661 cgatcatccc acggcgtctt tggttcttcc ccgagcacgt tcaccaaaca actgcggtgt
    15721 cctttccaaa tgaacacgtc aaaggaatca tctggaccgc actctccacc gtcggtgaga
    15781 ggctgtctca tcccgagccg attgacgtga tagtcccagc ctttttcgac atacgcgagc
    15841 gcctgtttcg cagtctgttc ttcttgcggt ctgtagtagt gaaccaacac cggcagcgtg
    15901 ctggaacgga tcgaatacgg gaacatctct gcgaagtcag ggcgcccgca gattttggtg
    15961 ccttccggaa gcggatgaat gtagttgtcc gtagtgtcca ttccgtcgct gccacagccc
    16021 atctgtaaga ccgccaggct gagtcctagc caaaatccat gacgcttccc agcgggccgc
    16081 atggtctgcg catcaacgtg atttgcatcg cttcttgttg ttcgcatcgt gcctccttgt
    16141 cgtttggggg gctttgcggt tgtgccagcg cctcgatggt tcgggttgcg aacaagggct
    16201 ctggcgcagt gcgatttggt aaaaaaggcg ggtcgatcag gaaccggacc ggctgttcag
    16261 aacgcgcgcg aatgcggcgt ctctgtcggc atagaccgtc tttacgtgcg ggcgttcaga
    16321 gacgagcttg aggtagtctg caagctgcgg aagttcgctg agcgcatcgc ttccaaacat
    16381 ccgcttggtc gtcatcgaaa tcagtgggaa gtgattcgcc gcggcacagt ctgccagcgt
    16441 gaattccgaa cccgcgagga acggatcaaa cttcacaagc tgtgaaaacg cgcgcaggcc
    16501 tttgtccaga tcacgcttca cttccgcttt ggtttcatcg gtcacatgac cgccgaaaaa
    16561 cgccgcggca agcaacctcc gcgagaccag ctcgacgtga agttccagca cttgggtaag
    16621 ctcccgcact ttcgcttttg caaagggatc gcgtggcaga agcggtgttt cgggatgcag
    16681 ttcttcgatg tattcacaga tgatctgtga ttcacagaga atgccatgct ctgtctgcag
    16741 aaacggaatc ttacccatcg gagagcgctg gcgaaactgt gcttcttggg aagggaatgc
    16801 tgtgatgtcc agctcgtgcg ggactccttt ttcgagaagc gctaggcgga ctttgttaaa
    16861 gtagttgctg atcggaaaac cacagagctt gagcatctcg actccttttg tcgttgggac
    16921 cgggacatta ctttacgctc tctgcttcgg cttctgcgag cgtcctgtga actgcgcgga
    16981 atcactctgt ttctgtgcgg cctgccgctt gggggactgc acttgcgacc gtggctgcgg
    17041 ttcggatggt tcgctgtttc cacagcagat agatcggcgg atagaccagc agctctagca
    17101 aaaatgacgt cacaagtccg ccgaccatcg gggctgcgat tcgcttcatg acatcggccc
    17161 cggttccctg tgaaaacaga atcggcagca gtcccagcag cgccgccatc acggtcatcg
    17221 ccttgggacg cactcttttg acggcaccat gaaccaccgc ttcgatcaga tccgcatgac
    17281 tgtgcagccg gtcttcctgt ttggcttcgt cataggaaag atccagatac atcaacatga
    17341 agaccccggt ttcggcatcc agcccgagca gcgcaatcac cccgacccag acggcaatcg
    17401 acgtatggta gcccaaaaga tagagcagcc agatcgctcc cacggctgaa aacggcaccg
    17461 cacacagcac gatcagcgct tttgtcaccg acttggtgtt tgcgtacagc aaaaggaaaa
    17521 tgagcagaat cgtaatcgga acgatcagcg tgagtcgctc gcgcacgcgc agcatgtttt
    17581 caaactgacc actccattcg atccgatatc cgctgggaag ctgcacgctg tgagcgacgg
    17641 cacgtcgtgc atcctctacg tagcttgcga tgtcacgctg ctcggtatcg aagtcgacgt
    17701 acacatacgc tgccagcatt ccgttttcat cgcgcaacat cgaaggcccg gtcgatagct
    17761 tcaaatccgc cacttgtccc atcggaacgg agttcccact gggaagcgga accaggacac
    17821 gcgaaagttc ggccaagtct tgccgcagct cgcgtggata acggagcgaa acggaatacc
    17881 gctcgcgccc ttcaattgtc gtcgtgacgg tctctccacc gatggcctgg gcaatcactt
    17941 ggtttgcatc ttccacagac agtccgtagc gagccagggc atcgcgacgc agcgcaaagt
    18001 cgataaaata tccccctgcg attcgctctg cgaacaccga tcgcgtaccg cttaccgatc
    18061 ggatcgcgga ctcaatctgc gcaccaatgc gatccagttc ggacggactc tgtccgtata
    18121 ctttgacgcc aatcggggtg cgaattcccg tcgacaacat gtcaatgcgg gccttgatcg
    18181 gcatcgtcca cgcattggtc gttccgggaa tccgcatcgc gtcatcgagt ttctgcacca
    18241 gctcttcatg tgagatgtga tcgggccaca gcggtcgtag cagcgcgttt ttgaccgctt
    18301 caggaatcgg ccagccggaa tagaactgat cctttttgcg ccacgctgat tccggcttga
    18361 gcacgacggt ggtttccatc atcgacagcg gcgctggatc agtggaggtt tcggcgcgac
    18421 ccgctttgcc aaacacggac tcgacttccg gaaaggaacg caaaatccga tcttgcagag
    18481 acagaattcg cagcgactct gccgcggaca cacccggcag cgtcgtcggc atgtacagga
    18541 tgctgccttc atgcagcgga ggcataaatt ccgcgccgag tttttggtaa ataggaacgg
    18601 tgctcagcgt caggaaaatc gccgacagaa tcgtcgtctt tggatgctga agtacaagcc
    18661 gacagacggg atcatacaca cgaaacagaa gccggctgac tggatgattt tcctcgcggt
    18721 gataggtgcc gaccagcatt cggttacaca gccaggccag cggtcgcggt gaaaaggaat
    18781 acggagtgac acgcgacagc atcatgcgca tcgctggatc gagtgtcacc gccagcagtg
    18841 cggcaatggc catcgccagg tttttgctca gcgcgagcgg acgaaacaag cgaccttcct
    18901 gatcgaccag cgtaaagact ggcaaaaatg ccaccgcgat caccagcaga gagaaaaaca
    18961 ccgacggtcc aacttccaga agtgcttgca aacgaaccag accaaagtcc ccctgactgc
    19021 catgttcctg ccacttctga atcttccggt aggcgttttc gacttccaca atcgcgccat
    19081 cgaccagcac accgatcgaa atcgcgattc ctgaaaggga catcaagttg gccggaacgt
    19141 gcagaagata aagcggaata aaagacaaaa ggactgcgac cggaatcgtg acaatgggca
    19201 cgatggccga cgggatgtgc cacagaaaca gcaggatgac gagcgacacg accacgactt
    19261 cgagcatcag atcatgcttg agggtggcca tcgctcgcag gatgagctcc gaccgatcat
    19321 aggttgtgac aatgcggact cctttgggca aagagctctc aagctgtgac agccgttctt
    19381 tgacgcgcgc gatcaccgac agcgcatttt cgcccattcg catcaccaca atgccgccaa
    19441 cgacttcgcc tttcccgtcg aggtctgcga ctccgcggcg cagctccggc ccaagctgca
    19501 cccgaccaat gtctgcgatc cgcagcggcg ttcccaagtc atttgtgcgc agcaccacct
    19561 gttccaagtc ttcgatcgat cggacatagc cgcgaccgcg caccatgaac tcttttccgg
    19621 aaatttcgac caagcgtccg ccgacttcgg aattggattt gcgcagcgct gtggctactt
    19681 cagatagact cagtccaaat gcggaaagtc ggtttggatc gacggtgact tggtactgtt
    19741 tgatcgctcc gcccacggtt gccacttccg cgacaccggg taccgactgg atggcaaagc
    19801 gcaggaacca gtcttgatag gaacgcagct cttcacgcgg agtctgacca ctttcatcga
    19861 ccagcgcgta ctgaaatacc caaccgacac cggttgcatc ggggcctagc tcggtcttca
    19921 catcgctggg tagcttggct tttattttcg ataaatattc cagcacccgc gatcgggccc
    19981 agtagatgtc cgtaccgtct tcaaacagca cgtacacata ggaataaccg aagtcggaaa
    20041 agccgcggat cgcttttact ttcggtgccc cgagcaagct ggtgatgatc gggtacgtga
    20101 cttgatcttc gatccgatca ggactctgtt cccagcgtga atagacaatc acttgggtgt
    20161 ccgatagatc gggaatcgca tccatcggta gctctttaag acagaaccca gcgacgacac
    20221 acgcaaccac agacagcatc accaccagca gtcgattgcg cgcacacagc gaaatcagcg
    20281 ctgcgatcat ggggcgtttc ctttttgcgc tgcgtgacct tggtgcgcgc tgtgatcttg
    20341 gtgcgcgctg tgatcttgat gtgcgctgtg atcttggtgt gcactgtgat cttggtgtgc
    20401 gctgtgatct tggtgtgcgc tgtgatcttg gtgtgcactg tgatcatctg acgcaggtgt
    20461 tggtgctgct ttggtatcgg tttcttcgtc gcttgtgcca ccttgcagcc gggcttcgga
    20521 atcgagcagg aagctggcac cttttgccac gacttcacct tccgaaagac cggcgcggac
    20581 ttcatagaga tgtccggatc gctcacccgt cgtgatcgtc cgctgcatga gcttgccttc
    20641 ctgatctttg acaaagacca gcttgcggcg tcctgtttca attactgcgt cgtcgggaat
    20701 caccagtgca tcgcggctta cgacttgtac ttcgacatca gcaaacatgt ccgctttcag
    20761 atcgccgcgc ggattgtcga cttcgatgcg aacgcggatc gtgcgtgtct tcggatcaac
    20821 cgaagggaag atatagctga gctgtccttg ccaagttcga tccggccaat agggcagggt
    20881 gatcgtcgct gtttgacctt tttctatccg cggcagttca tattcataga catcagccag
    20941 cacccaaaca cgcgacagat ccacgatgtc gaacagcgaa tcttcgggct tgattcgcat
    21001 tccgtgcact gcggtttttg cgatcacata gccactgatc ggtgcataca gattgaacgt
    21061 gcgactggcg cgacccgagc gttcaagctg tgcaatgtcc gacggactta ggttccatag
    21121 cagcagcttt tcacgcgccg attccaccag gtttggaccc ccatgcagac tggacagacc
    21181 ggcctgtgct ttgcgtgcaa tcaggtattc ttgttcagcg ctatacagct ctggactgta
    21241 cagcgcgacg atcggttcac cttttttgac aaacttcccg acgtagttgg cgtgcagatg
    21301 ttcgacgtag gcttcgtact tggtatggat gtgatgaacc agcgtttcat cgtatgcaat
    21361 cctgcctgtc gtgcgaatcg cgcgaccaaa cgcggcacgt tttgcactca cggtctgtag
    21421 accgatcatc gactgttgga ctgaatctaa attcagcgac ggggtggtct tttgcgaaag
    21481 gctgcgctgc ggtaccagat ccattccaca gatcgggcac ggacccggct tgtcgcttgt
    21541 gtactgaaga tgcatcggac acgtccagac ttctgctttg gcagacggac tcgattcctt
    21601 tgcgattcct ttgcggacag aagacagctc ccgatacaaa tagatcgcga ccaagctggg
    21661 cagaattccc accaaacaga gcagaaatag tttggatgcg cggatcattg cgaaactcct
    21721 tgcccgacgg aaaaatccag ctctgcacgg cgcagcataa gctctgccag gtagcgctca
    21781 tagtcgagcc gctgctgcaa aagtcgctgc gttgcggaca gcagcagaga cagtggaacc
    21841 cggccacttt ggtagcccgc gcgtgccgtt tgcacagccc gttcggacag cggaatcagg
    21901 tcatcatgat gcagtcccag atggcgaagc accgtctcac agcgagtata ggaagcgcgt
    21961 acttcggaaa gtacacttgc gcgcagctgt agttcctgtt gtttgttttg cgtgcgcagc
    22021 gattccgctt cctgctgggc cgccttggtt ttgaaaagcg aaaaggaagg aatggatgtc
    22081 tggattccga cggtaaacgt gcgttccatg tcgcttcgca gcggcaccat gtaacttccc
    22141 cacagtgcaa aatcaggagc ctgctcgctg cgcgcttgcg tgactttggc ttcctcttgc
    22201 tgcgtggcaa gttgtgccat tcgcagctcg ggccggttct gcgtggcctg ttcaagcagc
    22261 gagtccagcg acggcagtgg cacaagttca ggaatcgaaa cggggacgcc tagactccgt
    22321 ggggaagtct cccccagcag cgccagaatg tcactttcgg cctgcttgct tctgtgccca
    22381 agctcaatca gttcggtgtt ctgttcgtgc agcgcggctt gtgcatcgag cagctctgca
    22441 agctcagcgc ggcccacgcg atagcgacca tccaccgacg cgacgatcac ttgcaggatc
    22501 ttttggtttt cctcttttac cgcgagtgat cgatgtgaaa aaagatacag cgcataggct
    22561 cgcaccgtcg caagtcgtag ctgttgtttc tggagctggg tgtccgcttg cgcgcgcgct
    22621 tcatcgaaac gcgccacttg cgcgcgagcg accagcttgc ccggccaggg cagcgcctgt
    22681 ttcagcgtca ccatcagcgg cagccgttcg atcgaagtcg gcgcctgcca cagctcgacc
    22741 atcagcattg gatcatcgac ggacgcggcc tggcgcgcat gggttttggc agcggcttcg
    22801 gcggcgcgac gggaatgcaa aagcggactg tgcgcttcta cttcggacaa aagaccaggc
    22861 agcgtcaaaa ccggcggttc ggcgcggatt tctgacggca gaagcagcca tgcggacaag
    22921 cccgcaagcc aggtgcgaaa cagagcacgg aacatcggag tcctcccaaa gtcgaggcgg
    22981 agccaaaagc gaagcggcct agacgctggg gactacactt gcaggacggt gattccttgg
    23041 cggggtgggg caaaattggg cggaatgccc gacgaaaagc gcgtcacctg gggacgcgcg
    23101 ggcagatcat gcgtggtagc taggctacgg cttatcactg cgacgaggtg cgcgacctgc
    23161 tgcgccactt tgtgcggctg cgggccggac atgtcgatcg tcggcttcgg cagctcttca
    23221 cagcagtgat cgtggatcgt ggcgtattcg tgggtatcag cgatgctcgt cggacaacac
    23281 ggtgccgcaa tccgcgtccc cgccatcatc gtgcagcgat acgcgacctg cgtgggcagt
    23341 ccaaaacacg cccagacatg caggcagacc agcagcgcaa tcagccgacg aaaaagagga
    23401 accacgcaaa ctacccacta gcacaggaca tgggcgaaac acaacctggg gggacgaccg
    23461 cgctgacggc gagtgaccgg caaggtccgg acgcggtgca gcgcgcgatg tcaggggcca
    23521 cgggcgaagc cgtgaggcgc agtcaagtgg cgagaatgct aacgaggtcc gcgtccgccg
    23581 ccgccgccgc caccgccgcc actccgaccc tcgttgcgag gcgtgtgggc ttggttgacg
    23641 acgatcttgc gaccaccaaa ctgggtgccg tgcagctctg cgatggcact ttccgccatc
    23701 ttggcatcgg acagctcgac aaatgcgaag ccgcggggac gtccggtttc gcgatcggtg
    23761 atgatcttga cctgagttac cgagcgagaa ccagactcct caaaaaagtc ctgaatctcc
    23821 ttttctttcg ctccataagg caaattacca acataaagac gagttcccat agccacttcc
    23881 ctttccagcg cggcgttgcg cacaaagctg cccgatgcag gtatttggcc catcgtacgt
    23941 acccgcatga gtcctgcatt gactaggtta tgtggatttg ggggatttgc ctagcaggtg
    24001 gttgctgaag gcaaggtgac accgacggga aattgcctgt ttcatatgag aggaaacctg
    24061 cccgtttctt ccttcatatg ttgcaatctt tcgattggtg ggtatttcgc tattggaatt
    24121 gagaagcgtt tagctcgaca ttgcggtcaa gcttgctaaa gatcacttct ttttcgtcgc
    24181 cgctctgttc gaccatcagc aggcgacgca gcagcatcgt cttgccgtcg acgtgcagct
    24241 cgatccggct gaatactttg gcgagcaccg agtcgggctt gggcacaagg atcagcgccg
    24301 gctggtcgct cgttccgccg gtcttgacct cgtattcccc ttcggcgtcg cgcagcgagc
    24361 cctggccaag ccacagcttg agctgggcaa aaatggctcg cagcgtcgga tcttttccgg
    24421 tgtcgatgac ctgcggcgcg cgggagccca tcttcagcgt cgcttgctca cccaccaaga
    24481 gcatcgtcga tggatcgggc tttaggtatt cccagcgcag cctggccggc gtgcctttgg
    24541 cgtacagaaa gcggccttcg gagctgactt gctgacggaa cagcttgacc cgactgtgct
    24601 ggacaaagtc ggcccgcagc gagcgcaggt ctttctgcac ggcgtcgatc ttggtcatca
    24661 gttccgtcgg ggacggcagc ggcgcggcga agaccaggcc cggcgtgcag accagccaca
    24721 gcgacgccaa aaagaccaaa gcatgtcgcc tagcgatgcg ttgccacata gtcagccagc
    24781 gcagacgccg aggtgaaggc ttgtttgccc actttttcgt cgggaattgt gacgcggtag
    24841 gcgcgctcga tggccaccgc cagctcgagt gcgtcgatgg aatccagccc cagtccaccg
    24901 ccgaacagcg gcgcgttgtc ctcgatctcg tcgggggact tgtcgagctt gaggctgcta
    24961 atgatgagtt gcttgatttc gtgaacaaag ctgctttggg tcatgggttt ctcttcgagg
    25021 taagtacgga cgcggcgcaa aaacattcgc gcctagaagc tatcttctcg gattgcgtcg
    25081 agccctccgt cggcggcgag ccactgcccg gtgatgtaac ccgcttcttt cgctgccaga
    25141 aatgcaacaa gttctgcgac ttcgcttggc gttcctgggc gacccagcgg gatgcttgcc
    25201 agaagttctt tgcggacggg ctcgggaatg tctgccgtca gtgcggtgtc gacaagcccc
    25261 gccgagacac agttgcaggt gatctgaaag cgcgacagct cttgcacaag gcttttggtc
    25321 agaccaaaaa gtcccgcttt tgcggcccca tacgccgtct gtccgcgccg tcccagccac
    25381 gccgacgggg agcaaatgaa caggatgcga ccaaagcgcg ccgcaatcat tccttttgtc
    25441 accgcctgcg ctgccagaaa gccaccacgc aggtgcacgc ccagcacatc gtcgaagtcg
    25501 cgcaccggca tcgtcatggc cagcttgtcg cgcgtaaatc ctgccgcatg aaccagcacc
    25561 gcaatgtttt ttccgtcgag aaaaccgccc ggttggctga tcttttgcca gtcttccaca
    25621 gacgaggcat cacagcgcag cgcggcaagt tctacgcctt ctgctcgggc agcagcgacg
    25681 gtctgttcgg cttgggtggc atcgtgggca tacgtcacgg tcaccgcaaa cccgtcttgg
    25741 gcaagacggc gggccaccgc aagaccaatg cctcgcgtgc ctcctgtcac cagtgccaat
    25801 ttcatgacgg cgggatatta ctccgtcggc tgagcggtac aagtgcgcag aagtcggacc
    25861 gcataaaagc catcggcatt gtgcgtgtgc ggcaaggttc gcagcgcaaa cggtgctttt
    25921 ttttcacaca gcggcgatgg tcccgaaaaa aacagcggat cgtcgggcgg gggcggcagc
    25981 ggcacaaaat ccggatgtcg cgccaaaaac gccgcgatct gaagcggtcc ctcgggcaca
    26041 aacaggctac agaccgaata gagcagcacg ccacctggca caaccaagcc ggcggcccgg
    26101 tcgagaagct gactttgcaa tgtgaccaac tcgtcgatgt tgggtggccg cagccgcgct
    26161 tccggatgtc gccgcagcac gcccgaacca ctgcacggcg cgtcgagaat caccgccgca
    26221 taggccggac gcagcgccgc tttgtcatcg agcagatcgc aacaaatcgt ccgaatccgc
    26281 gtgcagccga gccggtgcgc atgatcgcgc agaagctcca gtttgcgcgg cagccgatcg
    26341 acggcgtcga tttcccgctg gttttgggtc agggctgcca catgacaggt cttgccgcca
    26401 accccagcac agccgtcgag aatcgccccc gcaggcaggg aaagcggacc cttttccgtc
    26461 gccgcaaaca gaagctgcgt cgtaagctgc gccgccaagt cctgcgcggt ataaagccct
    26521 ttggcaaaac ccggtcctga aaacggatgg ccgctgcgca gctccactgc cgtcgacaag
    26581 agcgcgtggg gcttgggcgg ttccggcagt tcgtctcgca gcgccgacag cgcttcatcg
    26641 cgcgttcccc gcagcggatt cagccgcagc catgtcgacg ccggctgtga aagcgctcgc
    26701 agcagcgcat cgacttcgcg cggatcgggc agcaccgcca gaaactgcgc gacgatcgaa
    26761 tcgggcagac catgatgcag cgcgagcagc ttgcaccact tgtcttggct ctgcgtcgct
    26821 ccgtcgggcg ggtagggcgg cagcggcggc tcgcccgtcg agtgcaattt gcgcagcaca
    26881 gcgtttgcaa aaccagccag gcccgcgccg cgtcgttttt tgatggttgt gaccaccgcg
    26941 tcgacgacaa agtgcggctg cgcgttcata aacagcagct gatacgcccc cagccgcaaa
    27001 agcgcccgcg tcgtggggtc aagcttgtca agtcctcgcg gggcatgggc gacgagcgcg
    27061 cgatcaattc gtcggacctg tcgcagcgag ccataggtca gctcggtgat gagcgcacgc
    27121 tccgtttcgg gcagcggctg tttggaaagc tcgcccgtca gcgccaaggt tgcgtatgca
    27181 ccctcgtcga cgcgcaacag cacccgcagc gccacatcga gtggtcgctg cttgggggct
    27241 cggctttcgg gggaagcggt cttatgcggg attggtggtt gcacttacgg tttgggctcg
    27301 cacatctctt cgggacggac cacgttgtca aactcagctt cggtgagcac gccaaactgg
    27361 agcgccacct gcttgagcgt cttgccttct ttgtgcgcgg tcttggcgat cttggcggcc
    27421 atgtcgtagc cgagcttggg cgccagcgcc gtcaccagca tcagcgagcg atccaccagt
    27481 tccttgatcc gcacttcatc ggcttcgatc ccgacgacgc agttttcgac gaacgagtgg
    27541 cacgcatcgg tcagaagccg caccgactga agcgttgcat acgcgagcac cggcttaaac
    27601 acgttcagct cgaagtgacc ttgcgccccg gcaaatgcca ccgcggtgtc gttgccgacg
    27661 acttgcgcgc agaccatcgt catcgcttcg cactgcgtcg gattgacctt gcccggcatg
    27721 atcgagctgc ccggttcgtt ttccggcagc ttcagctcgc cgatcgcaca gcgcgggccc
    27781 gatcccaaaa gtcggatgtc atttgcgacc ttgttgagcg ccaccgccgt cgtcttgagc
    27841 atgcccgaca gcgacaccac tgcatcgtgc gccgccaggg ccgcaaactt gttcggcgcg
    27901 gtcacaaacg gcagcccggt cagcgacgca atctcggcgg ccacttcgat ggcgtagcgc
    27961 ggatggctgt taagacccgt tccgacggca gttccgcccg ccgccagctc gtacatttgt
    28021 ggcagcgcag ctttgaccgc cgcgtgggcg ccccgcacct gcgccgcata acccgaaaac
    28081 gtctgtccca gcgtcagcgg cgtcgcgtcc atcaggtggg tgcggccaat cttgacgatg
    28141 tttgcgaact gacgactctt ggcctcgagc gcttcggcca gtcgaaccag cgccggcagc
    28201 agaccgtttg cgacccgatc gaccaccgcg acatgcatgg ccgtcggaaa cgagtcgttc
    28261 gtcgactgcg acatgttgac gtgatcgttg gggtggaccg gttttttgct gccgaggacg
    28321 ccgccgagga tttcactggc gcggttgccg atgacctcgt tggcgttcat gttggtttgg
    28381 gtgcccgatc cggtctgcca gacgaccagc ggaaagtgat cgtcgagctt gccgccaatc
    28441 acctcgtcgg cggcctgttc gatcgccggc agcagcttgg gatcgagctt gccgagtcgg
    28501 gcgttcgtgc gcgcggcggc ttttttgacg atgccgagcg cgtagatgaa ctcacgcggg
    28561 aagcgctcac cgccgatgcg gaagttttgc aaagagcgct gggtctgcgc gccgtagtag
    28621 cggtccgacg ggacttcgat gtcaccgaag gtgtcttttt cgatgcgagt ggatttgctc
    28681 acgacaaacc tcgttggaat gtgtgtggtg ccaaatgaaa cttcgcgccc gcgcgtatcg
    28741 cgtggctcgg ttattttgcg gcaagcacgg tagctgagac agcccgccgc tgtaaaggcg
    28801 tgggttcctg tcggagcgct gcgaccaaat cggccaagct gtcgaggttg tggatcggcc
    28861 tgaaatcatc gacatgcggc aaaatggcgc gaattccagc ggctttcggc gaaaattcgg
    28921 aaaaaccgag cagaggattg cagaaaacga accgtttcgt cgatttgtgc agccgttccg
    28981 cttctttcga caagtcgtat ttttcgtcgc gatcgaggcc atcgctgatc agcaaaaccc
    29041 acgcgccttg ccccagcacc cgccgcgaaa agtgctgatt gaagtcgtga agcgccgttg
    29101 ccagccgcgt cccaccgccc aagtcgtgca cctttaggct gatccgacgc agtgccagat
    29161 cgatgtctag tcctcgcatg ctctgcgtca cccgcgacag ctcggtgccg agcaagaaca
    29221 cctcgacgcg acgacgctgc gccatcagcg tgtgggaaaa acgcagcagc atttcgctgt
    29281 agcgcgacat cgatccgctg acgtcgcaca gcacacacag cggcggcgga agctgtatcg
    29341 gcttgcggta gtgcggcaga agcgattccc cgtggctgcg cagcgtgtga agcagcgtgc
    29401 ggcgaaagtc gaagcggctg cctttgggcg acggacgggt gcgccggaga ggccgcggcg
    29461 acagggcaaa gtccagcttg gcaatcagct tgcgtgcttc ggccagctct tcggcggtca
    29521 tgtcggcaaa atcacgctgg cgcagcgctt tttcggcgga ccagctcggc actccgtcgg
    29581 gaacgggcgg ctcgtcgtgg gcgtttgggg ccgccgcttg cctgccaaac agcgaatcgg
    29641 tcagccgtcg cagcagcgga tcgccaggcg gggacggcgt ttttttcggc atcggatcgt
    29701 cgctgtgtgg aagcggcctg cgcggttcaa aaaacagcgc aaacgcttgc tcaaacaccg
    29761 ccagctcttc gctgcgatgc accagcaccg cgcgcagcgc cgcccgcact tgctcacgcg
    29821 agcccagatc gatcacttgc agcgccgtca gcgcgtcgag caccgacgat ggaccaatcc
    29881 gcagcccggc ttttcgcaaa aggcgcgcaa actgcaccac gttggtggcg aaaaacgatt
    29941 cgacgggcgt catcggctag cccggtattt gcgctttgac ttcggcgacg agcttttgcg
    30001 caagcgggcc cttcactttg gcgatgtcgt cttggtattt cagcaccaca ccgagcgtcg
    30061 cgtcgacgga atccgcacaa acggcccgtc gagaaagtga aatcagcgct gccacccagt
    30121 cgagcgtttc ggccacgccc ggcggtttga acagatccag ctgacgcagt ctttgcacca
    30181 gggcgacgac ctgcgcagcg agctgcggac cagcctgtgg cgcgcgtgcg gagacgatct
    30241 ctagctcgcg tttttcagac ggatagccga ggaagtgata gagacagcgc cttttgagcg
    30301 cgtcgtggat ttcgcgggtg cggttcgacg tcaggatgca cagcggcggc gacggcgctt
    30361 tgatctggcc cagctccggc accgagattt gaaagtccga cagcacttcg agaagatagg
    30421 cgtcgaacgg ctcgtcggcg cggtcgattt cgtcgatcag cagcaccggc ggaccttcgg
    30481 gatgcggcga aagcgccgac agcagcggcc tgcgaatcag gtacttttcc gaatacagct
    30541 cttggctcag cgcctcgcgg tcgcgctcgc ccatcgcctc ggcaaagcga atcgccaaga
    30601 gctgccgcgc gtggtcccac tcgtaggcgg cttgcgaaag ctccagcccg tcgtagcact
    30661 ggagccgaat cagcgatcgt ccgagcgctt gtgccagcac cttggccagc tcggttttgc
    30721 cgacgccggc ttcgccttcg ataagcagcg gtcgggaaag ccgcaaagcc aaaaacagca
    30781 ccgtcgaaag ccccacatcc gcgacgtagc gctggcgggc caaaagctgt gacagatcgt
    30841 cgacggatgt ggggatctca gtcatcgaat cagccgccga gtgctgcttg gatggcgcga
    30901 cgggtgatga ccgggatcag gtgggcgcga tattcagcgc tcgcctcggg cgtcgacgta
    30961 agctcatcac tcgggtagga aagtccactg atcgcgtcgg gcgaaaagcg agctgtcagc
    31021 gcagcttcgg cgtctttcca gcggaacgcc gacggaccgg ctccggtgac accaacacga
    31081 acgccagaag cgaacttggc gaccatcaca ccgacgaccg cgtagcgcga ggcatggctt
    31141 gcgaacttgc cgtaggccga ccgctcgggg atgggaaagc gaacgctgac gatgatttca
    31201 tcgggttcga gtgccgtctc gaagagtccg gtgaaaaaag cgtcggcagc gatggttcgc
    31261 ctgtcggtgt tgatcgtggc cgaaagtccc aagatggcgg ctggataatc cgccgccgga
    31321 tcagcgtggg ccagcgatcc cccgagggtg ccgcggtttc tgacctgtgg atcgccaatt
    31381 ccgtcggcaa gcttggcaag acctgggatt ttggcgcgga catccaaaga tgctgcgacc
    31441 tcagcatggg tggtaaaggc cccaattgtg acctgctgac cgtcgctgcg aatgccgcga
    31501 agctccgtca cgccagccag tgaaatcaga tcactgggct gcgccatgtc gagctttagc
    31561 accggcaaaa aactctgacc accaccgagc agcttccccg cttccgtgct ctggagaagc
    31621 tgcttggcct gagcgacggt ggagggacga tgaaactgaa agtcacgcat gtcgttctcc
    31681 tttgtcgctt acttcgcagc ctgcgctgca gaaatggcac gccacacctt ttcgggcgtc
    31741 gctggcattg aaatgtcggt cacaccaagc ggcgacaggg catccaaaat cgcgtttatc
    31801 accgcgggtg ggcagccaat cgcaccgact tccccgacgc cttttgcacc gacgggattg
    31861 tgggtacacg cggtgacgtg gttgccgact ttgaaaaacg ggaaatccga cgcgcgcggc
    31921 atgccgtagt tcaaaaacga cccggtcaga agctggccgc tttcgtcgta ctgcgcttct
    31981 tcgtagagcg cttggccgat gccttggacc agtcctccgt gcatctgtcc gtcgacaatc
    32041 atcggattga tgatcacgcc gacatcgtcc gcagcgacca tttcgacgac ttcgaccact
    32101 ccggtgtcgg gatcgacttc gacttcgcag acgtggcagc ccgccggata ggtgaagttt
    32161 ttcgggtcat aaaacgccga ttcttccagc cccggctcga ctttgtcgat cgggtagttg
    32221 tgcggcacgt aggcggcaaa cgcgatctcg ccgatgctct ttttgcggtt ggtgccgacg
    32281 acgcggaact cgccttggtt ccactcgatg tcggcttcgc tggcttcgag caggtgggcg
    32341 gcgatctttt tgcctttggc gacaatcttg tcgaccgcct tcacaatcgc tgatccaccg
    32401 acggacagtg aacgcgatcc atacgtgccc atgccaaacg gaatcttgtc ggtgtcgccg
    32461 tggacaattt cgactttgtc gtaggggacg cccagcttgt cagcgacgag ctgcgcaaac
    32521 gtcgtttcgt gtccttggcc gtggctgtgc gtgccggtga agaccgaaac tgaaccggtc
    32581 ggatggacgc gcaccgtcgc ggcttcgtac agtccagcgc gagcgccaag ctgtccgacg
    32641 agagccgacg gggcaattcc acatgcttcg acataggtgc taaagccgat gccgcgcagc
    32701 ttgccgcgtt ttttcgacgc ttcccgacgg gccgaataac cctggtaatc gaccgctttg
    32761 agcgccatgt cgagcgtcgc ctgatagtcg cccgtgtcgt attccagtgc gacgggcgtt
    32821 ttgtagggga actggtcttt gcgaatgaag ttgcggcgac ggatttccag ccgatcgatg
    32881 cccagttcac gcgccgcttt gtcgaccaac cgctcgagca gataggccga ctcgggacgc
    32941 cctgcgccac gataggcgtc gacgggaacg gtgttggtga agaccgcttt gacctcgacg
    33001 taaatcgccg gagtgctgta gcagccggcc agcagcgtcg cggacagata ggtcggaatt
    33061 gccggcgcaa acgtcgacag ataggcgccc atgttggcga cgttgtgaac acaaagtccc
    33121 aaaaatttgc cgtctttgtc gacggcaaga tgcgccttca tcaccgtgtc gcgaccgtgt
    33181 gcgtcggaca taaacgactc ggatcgttcc gccgtccact tgaccgggcg cccgatcttt
    33241 ttgctcgccc acgtcacaat tgcttcttcg gcgtagtggt aaatcttcga tccgaagccg
    33301 ccgccgacgt ccggtgcgac gacgcgcagc cggtgttccg gaatcccgag cacaaacgca
    33361 cccaacagaa gccgaatcag gtgcggattt tggctggttg tgtacagcgt gtagccgtcg
    33421 ctggctgcgt cgtactgacc gatcgcagcg cgcggctcga ttgcgtttgg aatgaggcgg
    33481 ttgttgcgaa cctcgatttc gacgactttg taggccgact tcatcgccgc cttgaccgcc
    33541 gccttgtcgc ccagatccca gtcgaagcag acgttgcctt tggcctgggc ccagaccgtc
    33601 ggcgctccgt cagaaaccgc atcttgcagc gtcgcaaccg ctggcagaac gtcgtaatcg
    33661 acttcgatga gctgggccgc agcgcgggcg tcggctttgc tttcggcgat cacaaccgca
    33721 acctgatcac cgacgtgacg aacacaatcg gcaacgagca tcgggtgcgg cggctcgacc
    33781 atgttggagc catccttgtt tttgaccagc cagccacacg gaatgccccc gactttgtca
    33841 gcgacgacat cttggccggt gaagaccgcg atcacgccag gtgcggatcg cgcagactcc
    33901 gtcgagatgg aacgtatctg cgcatgcgcg tgcggcgagc gaacgatata cgcataagcc
    33961 tgattgggca gcacgatgtc atcgacatag cgccccgcac cggtgataaa acgtcggtcc
    34021 tctcgacgag gaatcgcggc cccaatcgat ccggaagact gagtggtggt gctcatgcgc
    34081 ttttctcccg catcgcatcc cgacaggaca ggatggcttt gacgatgttg tggtagccgg
    34141 tgcagcgaca gaagttgcct tccagccact cgcgcgtctg atgctcgtcg gcatcgggac
    34201 gtttttcgac cagctcgacg gcgctcatga tcatgccggg cgtgcagaaa ccgcactgga
    34261 gcgcgtggtg tttgtgaaac gactcctgca tcgggtggag cttgccggct tgggcgagtc
    34321 cctcgatggt ggtgacgcta cagccatcgg cttcgacggc aagcatggtg cagcttttca
    34381 ctgatttgcc gtcaacgtgc acgacacaag cgccacagtg actggtgtcg cagccgacgt
    34441 gcgtgccggt catctgtagc ttgtcgcgca gcagctcgac gaggagcgtg cgcggctcga
    34501 tatcgaccgc gacctgcttg ctattcacct tcagagagac ccgaatcatg gttcctccaa
    34561 gctcatgccg taggtgccgt cagaaacgtg ccagcggacc atccgccgcg tctgcaccct
    34621 ggttcccggc gagtatacaa aactacaaat tcgcaagggc agtcttgaca tgcgtcgctg
    34681 gtcgatgtcc gctctgtggc ggaaaactcc gcgagtcgtt aggctaactt ctcaaacgga
    34741 gcacaaaaca tggcttcgct gcgcatttcc atactggctg tccctctgtt tttcgtcgct
    34801 ccggcatttg ctgaagacgc cgctccgtct gcgctgacca cagcgacgat gaatcctgtg
    34861 gtcgccgacg cgggatggaa gtttgcgtcg gacaaaggcg gcgtttccgt ctggtttcgc
    34921 ccgtttccgg cgtcgtcggt caatgaagtc cggggcgaag cggtcttcga tgttgcggcg
    34981 gacgcgctgt ttgcagtcct tggcgacatc gacgcctatg ccgaaatcat gccgccaacc
    35041 tcgctgtcga agcgactttc cgacggtacg agcgcgcgaa gttattacat cgaaatcgat
    35101 ccgcccgtgg tgtcccgtcg ctattactgc atggatgtgg tgctttcacg gccccgcgtc
    35161 gatcagtttg tcagtgagtg gcggatgtgg ccgcagggct gctcgccgcg cgcccgttcg
    35221 atggtgcaga tggccgacaa cagtggacgg tggcgtttgt cgtcgctggg gccgctgaaa
    35281 accaaggttg aatttcaagc ccacatggac cccggcgggc aaattccgac gtggatggta
    35341 aatcgcacga cggtgggaca gattgccaat acgtttgcgt cgctgcgtcg agccgcccag
    35401 tcgccccgct atgcagcgat gaccaagcca gaaccccaag gggaaccaga gcgtccgtcg
    35461 gtgtcgagca agaccaactg acaaaaacga atcgaactag cgagcaacca gtaccagcga
    35521 cgggcctttg gtgtcgatgg gcggctcttc gatgtcgaaa gtggtcgtga gcacggttcc
    35581 aatcggctcg ctgcccagtt caggcggctt cgctgggatc ggtttcgccg tcggcttggc
    35641 ggttttgtca gctcttgctt gtggcttgat cgctgcaggc tgcgccgtcg gttgtttgag
    35701 cagcaatgtc ttcatttccg gcaacatgtc actcgggaca aggcacgaaa acagctcctt
    35761 ttccccgatc gactgtttga gggcatgcac actctcgaac tgctcgattt gccgtcgaga
    35821 atagtaagac ggcacggtgt tgctcattcc aacgacacag cggcgggctg tcggcggagc
    35881 atggcgcagc gactggacca gcgtcttgat ggtgtcactt tgatgcagta ccggatcgcc
    35941 ttcgaccacg gccagcacca cgccagcgag agccagcgtg gtgatgattc caccgagaga
    36001 acccagccag cgaacggggg actgaagccc gactgcagcg gcaagcgcca gcggcggcaa
    36061 cacaagtcca agcccacgcg gtggtgccag atcgccgccc acccaagtca gcagcgtcgc
    36121 cagcatcacc cacacaagga gcggtcgcga ggcacgccgc atcgatccca aaagcagacc
    36181 aaagcaggtc gtgaccacga caacctgtcc gacgtgacgg ccataaaagc tggggccttg
    36241 tcgccacacc gcgtcgaacg cctgcataaa aatgccaggg gccggacgat gaaacagcgt
    36301 tcgcagcgcg ccgccatgaa tcgccgacgc accgagcaga agcagtgtgc caccgagcca
    36361 catcagccaa acccgtcgct tgcgcagcag gtagcgatgc gagatcacac agtcgaaaaa
    36421 accaaacagc agtgccacaa gacctgcccg cacgtcgagc acgcccgaaa gaccaattcc
    36481 cagcgccgca aacagcatcg gcaggcggcg cagcggcagg ggctcgggtt cctcggtggc
    36541 tttttgcatc ggcaggatgt gacggtcaaa cttgtggccg tgcagactgt ggctgaccag
    36601 cgcgaccgcc cctgacgatg ccaccgcgac gagcatatcg ggcagcacgc ggtgtgaaag
    36661 ttcataggtc agcggcatcg ccaaaagcac aagacctgcc agtccgccgg tgtttcgtcc
    36721 gattccgacg tcgatcgcaa tcgccagcaa gcagctcgcc gcgccaagtg ccgccagcac
    36781 cgggacgagc cgcagggtcg cttcgctcat cccgaacacg cggcttagca gcacaaaggg
    36841 cacgataccg atgggcccgc ccgtcggggg cggcacactg cggaacgctt cattccatag
    36901 cagttgcccg gcgagcactt tgcgcacgac aagcgccact tccgcgtcga gcagcgacag
    36961 acttccggcc cccaagtcga caaagtacag aacgatcggc agcagcatcg ctaccgccca
    37021 gccgagcacc agccctttgg gcactggcag cgccgcgacc gcttgttgca ccgtaccgac
    37081 gggatcgcgc agcgcgttcg cggtcacatt gatgccgcgt tcgatgccct cgccgagctt
    37141 ttcaggcagc ttgccgggca gggcggccag ttccgccaga cggctccgcc ttcgttgtcg
    37201 cgaaacattt ttggctgtcg acggtcgtga cagcgttggc ttgccactga cgggacgacc
    37261 cgagaccggt agcttgcgcg ggcccgaaac cggcagatac gatcccgatg tcacaccgcg
    37321 acgacgaact cccggctgcg aacgcgaaac gaagcccggc tgagacggtg aaatcgcctc
    37381 gtcttggctg tctcccggct tgtcgccgtc gtctcgcttc ccgctcacgg caccgattct
    37441 agtgctagtt ggctacgctg acgacgttca gttcgcgaat cacggtgact ttgatctggc
    37501 caggaaagac caggttgtcg ctgatccgct gggccagctc ggacgccatc tgggcggcgc
    37561 gcaggtcgtc gacttgacct tctttgacat ggatacgaag ctcgcggccc gcttgcagcg
    37621 cataacagcg ttcgacgccc acagcaccgt ggaccagtcg ttccagatcg tcgatgcgct
    37681 ggatgtggct gccttcttgt tcgtgtcttg caccagggcg gccaccgctc atggcatcgg
    37741 atgccgcgac gatcagtgca tacggcgaat tgaacggttc gtcgaggtga tgggcgccaa
    37801 tcgcgttggc gaccagttcg gtttccccga gccgccgcgc ataatcggca ccgatcaccg
    37861 cgtggctgcc gtcgatcgca tgggtcagcg atttcccaat gtcgtgcatc agtgccgccc
    37921 gtcgcgcaat cttcatgtcg agccgcagct ctgccgcgag catgccacac aggaacgccg
    37981 actcgacggc gtgcttccac tggttctgac tgtacgacgt tcgatagttg agccgaccga
    38041 cgagacgaac gatgtctgga tgggcgcgcg gaatttccaa ttctttgaat gcgcgacggc
    38101 ccagctcaac ggtctcgcga tccaaatctt ctgccaagct ggtcgcaatc cgtcgcaccg
    38161 cttccggttc ggcgcgggtg ccttgtttga taagccgcgc cagcgtccga cgggaaattt
    38221 cgcgtccaaa gctatcctga ccttcgagtc gcaccgatgt gcggtcgtcg cttatcgcca
    38281 gcttgatgtt ggccacttcg ccaatcaccg tcatgacatc ggcaccgtcg ccaaacaccc
    38341 gatccagtat ctcggcggga atcggcagcg tcgacagcag tcgttcggtc aggtagtgcc
    38401 cttggtaacg ctggctcgaa atttcgagca gccgcttggc ccggcccgcc gtctctggcg
    38461 cattttgcgt ttgttcgagc tgccgcacgc gatcggcccc agcagcccgc gcctgttcgg
    38521 tccagtcggc gatcaggcgc gctttggcgt cggcaaacgt ctcgcccgcc tgtttttgta
    38581 gcaggtcact tgcctgctgc tgaagcgtgg cgcgtcgctc ttgggccgct ttttgtcgcg
    38641 cttgcgagag cgatatcgct ttttcccgct cgttgagcga cgcaattctc gtctccagca
    38701 tttgttcggt gcgctggagc gtctgttccc acgccgccac ctgttgcgcc ccacgcttta
    38761 gcgtttctcg tcgctgctga aagtcgcgtt cggcctgttg gactttgcgc aacttctgct
    38821 cggcagcgtc ggaccgggct tgcgtcagct gtttttctgc ttgggttcgt gcgtcatcga
    38881 ccagttctgc ggcgcgcagc ttggtctctt cttgaagccg attccactcg gtgatgcgct
    38941 gcttgcggag gtaggcaaaa accaccgccg caagaacaag cgacagacag acaaacagag
    39001 gcgtcacaag ctgaatcttt gccgtcgatt gcggcccacg tcaaggttcg gcggtcttga
    39061 cgtgttggct ttgcttgtgg cacacaggcg ccgcctgggg aggcccgata tgaacccgaa
    39121 gcgaagtgca tccaaacaaa ccctgtattt gttcgtaact ggcggtgtgg tttcttctct
    39181 cggaaaagga ctggcttcgg cgtcgattgg cgcccttttg gaagcgcgcg gtctgaaagt
    39241 cgcaatccag aagctggacc cgtacatcaa cgtcgatccg ggcacgatga atccgctcca
    39301 gcacggcgaa gtgttcgtca cggaagacgg cgccgaaacg gacatggatc tggggcacta
    39361 cgagcgcttc atttcggcgc ggatgacccg gcgcaacaac ttcaccaccg gccaggtcta
    39421 cgaatcggtc attcgcaaag agcgcaaagg cgaatacctc ggggcgacgg tccaggtgat
    39481 tccccacatc accgatgaga tcaaaggcaa catcatcgcc tgctccgaag gactcgacgt
    39541 gctcatcgtc gaagtcggcg gcaccgtcgg tgacatcgag tcgctgccgt tcctggaagc
    39601 gattcggcag cttcggcacg aagtgggtcc gggcaactcg gtggcgatgc atttgacgct
    39661 ggtgccgtac atcgcagcgg cgggtgagct gaagaccaag ccgacgcagc actcggtgca
    39721 aaagctgcgt gagatcggca tttcgcccga catgctgctg tgccgctgcg atcgtccgat
    39781 tcccgaaggt cagaaaaaca aaatcgcgct gttttcgaac gtgccgcaag acgcggtgtt
    39841 tacgtcgcaa gatgtcagca ccgtttatgc cctgccgctg catctgcacg agcaaggtct
    39901 cgacgacaag ctctgcgagc ttctgaacat tcgctcgcgg gcaccggcgc tttacgaatg
    39961 gcagtccatc gtcgaacggg tcatccgtcc ggcgcatgaa gtcacgattg gcatcgtcgg
    40021 caaatacgtg gacctggtcg aatcgtataa gagcctgcac gaagcgctga tccacggcgg
    40081 cattgcccac aacaccaagg tccgtcagcg ctacatcgac gcggaagaag tcgaacaaaa
    40141 gggcgccgag cacttccttt ccgacgtcga tgccatcttg gtgccgggcg gttttggcaa
    40201 gcgcggcgtc gaaggcaaga tcgcggcggt tcgttatgcg cgtgaaaaac gactgccgtt
    40261 tttcggcatc tgcctcggga tgcagatcgc ggtgatcgaa tatgcgcgga acattctcgg
    40321 gcttgcgcag gcaaactcgc tggagtttga tcccgacgcg ccgcaccgcg tgatcgacct
    40381 catgaatgag cagcgcagcg tcgaaaacct cggcggaacg atgcggctgg gcgcgtatct
    40441 tgccaagatt gcgccgggca cgttggcgca tcgcatttac ggcaccgacc aaatttccga
    40501 acggcaccgg catcgcttcg aggtcaacaa cgcctatcgg gatcagctgg ccaaggcggg
    40561 acttacgctg tcggcgcttt ctcccgacgg aaaactcgtc gaaatggtcg aaatccccga
    40621 tcacccgtac ttcgtcggct gccagttcca tcccgagttc aagagccgcc cgcacagccc
    40681 gcatccgctg ttccgcgagt tcgtcgccgc cgcgctcgtc cacaaaggca agcggaacta
    40741 agcgagcgtc ttggcgccgc gctacttcca ggtaaattcg cgctgcgctt cgccaagctt
    40801 caccgtcaga gtgccgcctt tttggttgga ccaagtccaa ccgctggcgg tcatgtccaa
    40861 gtcgggtgac gtgccggcgc gcatcagcac ttcgtttcca gcggtcactt gcttgggttc
    40921 cgtcggcagt gggtacaccg tgatcacctg gcctttgaca aagacgctgc cggggcttat
    40981 cgaatagtgc tgaccgacgg ggtccagctt gattcgcgtg ccgtcgtaga cggtaaactc
    41041 gctcgccggg ccgccgaata tggcgacgac gtacagtttt cccggatcgc gcgcaaacga
    41101 ttccaccttt ggatctgtcg tcggtgacag cgtatcgatc gccggatgca gcagcggcaa
    41161 aagcgcaccc gctcgggcaa acagtggcag cttggaaagc ggcgcagcaa cggtgatggt
    41221 tcgtgggttg tttccgggga agacctcgcc gctaaaaaaa tcgacccagt cgccgggcgg
    41281 aagcaacacg tcgcggctgc gggcaccgcg aacgagcacg ggcgcgacca gcaggtcatc
    41341 gccgaacaga tactcatcgc tcgggtgctg accgagccgt ggatacgcga gccccaccgg
    41401 tctttggatc ggtcgtcccg tctgtgcaag ctcctgcgcc agcgtccatt catacggaaa
    41461 cagccgcaga tgcagcctgg cgtactctcg atacagatcg agcgtttctt ggtcgcgtcc
    41521 attttccggc gtaaattccc acggcgtctg gctcgtcgag tcgcccacct gcatcaccga
    41581 cgacagcgcc gtctgctgaa accagcgaat gtacagctct ttgtcgggcg gactgtgacg
    41641 gaaaccgccc gtatcggcgc caaagaacgg gaatcccgac acacccaagt tgattcccat
    41701 caccaccgtc gctggcagac ctccgacgct gagatacgtt ttcccgtcgc ggtctttcca
    41761 cggctgccgg tggctcgcaa acgtcgcatc catgtcgcct ggccagatca ccgacacgtt
    41821 tttttgatca ccaaagcgtc ccgtccgaca cagcagaaac ccacccgact tcggcaacag
    41881 ctcggcatac gtttcgtgat agcgcagttg atagctgtgg tgcatcgtcc gctcgtcgct
    41941 gccatcggaa aaatgccagt ttgaacgcgc cgccccaaga cccaccatca cgtcttcgcc
    42001 gtaatcgagc ttgaaaccct caatcccttg ttcgatgtag cgtccgacgc gctgctgcca
    42061 aaactgcttg gcggcgggat tggtaaagtc gatgggctcg ctccagccgt tgagcagcac
    42121 gccgctcgac gggggaaagt agcctttttg tttggccaag ctgagatcgg gactgccttt
    42181 ttccagatac ggcgcatgcc acagcgcaag tcgcagcccg gcagcgtgca ctttggcaat
    42241 catcgacgtc gggtccggaa aacgcgccgc ttcaaaatca aagctgttta ccgtcgtcgc
    42301 atagggccta tcgatccaaa ttgcgctcgt cgccaagtcg agatcgcgaa tttttttcag
    42361 atcgtcttcg agctgcgctt ggtttttgtt ttcattgcgc caaatccacg gaccgagcgc
    42421 ccacgtcgct ggcagcagcg gtttccccgt gatttcgtaa tagcgcgaaa gcacagagct
    42481 gggctcggca gcggtcagca ggtggaagcg caggccattt ttgctgtcgt cgctcgtgcc
    42541 aaatgtcgtt tcgaccaagt cggcttgctt gcttgcgacg gcaaatacac ctgggcgacg
    42601 gctttcgaca aaaagtcccc agccgcgggt gccgataagg agcggaatcg ggacgcggat
    42661 ttcgttgctt tttgactcga gcacatcgat ttcctgctgc atcggacgga ctttgccgcg
    42721 gtggctgacc tcatcggccc agccgccgag accgtagaaa ccttcgcttg cgtcggtgcg
    42781 cggtcgcagc ctgatgtacg cgacgggccg ttcgctgcgc acgtcgggaa caagctgggc
    42841 ggcaaaacgt ccgggtccgc ttggcgcaat ctccagacgg gccttctgtc cgtcgctgag
    42901 cacaagcgaa agcgtgatct ggagattggt tgcggagtcg atcgaaatgt ggtcgacgtg
    42961 atgccaaatc atgtccgcgg gctcacttgc ggacagcacg tcgtcgccgt attcgagcca
    43021 atagggatca aagctggatc tgtcgtcgag cttttcgacc gtcgccacct gccatgcgtc
    43081 cggatcgaag cgcagaagcg tctcgtcgcc acgtacaagt cgcaaggaac tgctcgacgg
    43141 atcgaactcg acggcactgt cgccatcgct gacccgcacg ggcgtgagac ttggggtctg
    43201 gcagccgatt cccatcagcc cgatggcgac gaggagctgc gggatttgct gggcgcgggg
    43261 aacttgtttg ccgatctgcg gtctgtgctt tctggcggct ttggcacaca tgaccgcgag
    43321 caaatcacga gcgcggcgtg gtcggaaaga gcggagtcag gtgcgctgtc tgtctgctgg
    43381 ctgggcctgc taagatggga agtaagcttc aacaaggaga acctacgatg cacaagcctt
    43441 tgctcacctt gctggtgggt ctttcgcttg tctcggcgtg cggtgcgccg acgaaaattg
    43501 cgcaagttca cgatgccaac attttgccgc tgcggtcggt gcggctttat gaaacgggca
    43561 tcggctactt tgaacgggcg gggacgttgg gatcacgggc gacggtgctg ccggtgccgg
    43621 cgggtcacat cgacgatgcg ctcaagacgc tggtggtgct gtcccgcgat cagaaaacgc
    43681 gggtgttcgg tctggagttt gcgagcagcg taagtccggg catggggcgt gcgctcgccg
    43741 gactgccgct ttcggaagac gatccgatcg attttttgca tcttctgcgc tcgtgtaagg
    43801 gcggcgatgt cagcgtccgc gtcggtcagc aaagcataaa tggccggctg atcgacatcg
    43861 tggaacacgg tcacgacggt ccggtttcga ccgccgcgag cgaatccaaa aacgatggca
    43921 aggaccagcc cagcgatgga aagtcgattc gggcgcagaa tccatccgat ccgcagcttc
    43981 tgcttttgtc gcaacatggc gagcttttgc ggctgccgct cagtcgggtg acatcggttc
    44041 ggccccttga tccatcgttt gcgccgagac tgaggacggc gctcgatgcg acgtcacaac
    44101 gaagtgccaa cgcccagcga ctgattcggc ttttgagccg cgacggcggg ccggtcacca
    44161 tcgggtacgt cgccgaagcg cctttgtggc gggtcagtta tcggctggtc gtgtcggaaa
    44221 ccggcaaagg tggcgtgctg caaggctggg cgctgattca caacgacacc gacgaggact
    44281 ggcaaaacgt ccaggtgcat ctggtcaatg gtcgccctga ttcgtttttg tttccgctcg
    44341 cggcgccgcg ctatgcaaga cgaacgctga tcacgccgga caaccagttg tcgacggtgc
    44401 cgcagcttct cgggcagagc gccgaccagc tttggggcga caatgtcggc gatagcttcg
    44461 gcgtcggcgg acttgggctc agcgggatgg gggctggcgg cggtggcaga ggcgaaggtt
    44521 cgatcggtct gggatcgatt ggcaccatcg gccacggctc tggcgtcggg accggcggca
    44581 cgggttccag ctcgcttttg caagtgggca acttggccgc cactgccaag tcgaccggcg
    44641 tcgagtcggg cgcacttttt acctattcgc ttggcgaacc actcgagctg cgggcacacg
    44701 gatcggcgct ggtgccgttt gtccaagatg cgatcgaggt cgaaaccatt acgtttatgc
    44761 cgtcgccggg cgagcccgcg cgaagtggtc tgcggtttac caattcgacg cggcagacca
    44821 ttccgggcgg aacgctggcg gtgctgggtg aaacggggct gctcggggag tcggcgctgc
    44881 cgcggctgaa accgtcggaa cgtcgcttca tcgagtatgg cgtcgagctg gatgtcgaag
    44941 tctcgcgggt ccgacagacg cagaaagagg agccacagcg actgacgttt tccgacgatg
    45001 agttcgagga gcactttctg cgcaagcgcg accaactgct gcgcatcgaa aatcgcagtg
    45061 gccagacccg tcagctgtac ctggcgctca atctggcggc gaattcacag gtgcaagggg
    45121 ccgatcgact cgactttgat cacgaaaaaa aatcgccggt cgcgattttt gaactggcac
    45181 cgcagagtcg ggccgagcgg ccacttacga cgatggaagg tcttgtgcga cggtccgatc
    45241 tgcgatcgct acaagcggca cggctgctcc agctttcgac gacgagcacg gtgccagcga
    45301 cggagcgatc gatcgctgca gaagcgtatg cccgtcagaa ggacgtcgag gacgcggcca
    45361 aggaagtcga caaagtgcgc ggcgagatca aaaagaccga aaaggatctc gagcggctgc
    45421 gcgaacacct caaagcgctc ggcaaagatt ctggcgccgg tcccgaggga aatccactcg
    45481 tgaagcgaat cctagaagcg gaagatcgtc tgtcggcgtc gcagaaacgg ctggaatcgc
    45541 tcgaatccga tcaagatcgt cgtcgtgacg cggtgcgcaa agtgctagag cgactgccga
    45601 aaaacaaaga caaatcgacg ggaggctaaa taccgacggg gcgtggatta gctgcgacga
    45661 gcgatgcggc cttttccgtc gaccgttgca tcgatgccaa gctcgtcaat cactcgctcg
    45721 acgctggtct tgtcgctgac tgacaatcca gcgtcggcca tgccttcgac aatccgaatc
    45781 ttgatgcggc ggctctcttc ggctttgttg agcagcagtt tgcacagcgg ttcagcgacg
    45841 acatcggctt gggcttggtg caacagcgct tcgacggcct taaaccgcac gccttcgtca
    45901 atgtcttcga ggtacggcag cagcagcttg gcaatctgcg ggtcgcggtg ttcacccata
    45961 aagtgcacaa gctgcgtctt tttgctgggg tcgcgcacgt aactgttgtc gttttgctcg
    46021 cagagctgtt ccagcgcttt cagaaagtca tcccctttgg cgacctgttc cagcactttg
    46081 agcgcccatc cgagcgtctc gctttcgcgc atgtacttgc gcagcggcga aaggactttg
    46141 gcacccatcg cgacaagctc gtggtagacc cactcttttt cctgctcgtc ttcgatggtt
    46201 ttgtcataca cgaagctgaa gcggcggagc agtccgatga tggcgtcggc acttccgtcg
    46261 tcacgcagaa gttcaagggc gcgaaaacga tcgggggact gcgcgtgctt gtcgctcgca
    46321 cgctgaatca tcttctggag gctgcgggcc tgccgcccct ccttgctgaa atagtctgct
    46381 agacccaaag tcgatacctc actgcacaac tagttaaggt cgccaaaaag gacgcgctag
    46441 ggagaaaatg gctgcggcga gcaggaggcg cgtttttttg ccggctattc cttgcagagc
    46501 tccatgcctc gctgggcagc ggggttcgag ctgtcggcgc cgttgaggat tttggcgtag
    46561 taaaagcgcg cttctttgca gtttttctga cggtaatagg ccttgccgag cagcaagtag
    46621 aggtcagtcg tcgtcgcctt gtttttgagc gcttgcttgc cccacttgat cgcctcgccg
    46681 aatcgtcctc gaccaaactc cgcttcgccc atgcattcga gcgccaggcc gcgcacctgc
    46741 ggatttcccg ccgccgcata gcagaggttg aagccgactt cgttgcgatt ggcgttcagc
    46801 gcgagccgtc cttcttcgat tttcccgagc gccacgttgc gatcgataaa cggcggcatc
    46861 gtcaagttgc cgagctttcc cggtttgccg tccccgtcat cgcggtcttt cttcggtggc
    46921 actggcgtct tgcccggatt ggtttccgtc gccgcttgcg gtggcttggt cggtgtaccc
    46981 gcgtcgggtg gactgaccgg cggattcgtt gccaccgccg gtttaatccc agcatccacc
    47041 acaggcggtg tcacaagttt caccgctcct gcgtcgggat ggggtttttg caccggggcg
    47101 gtgaacaccc aatacagcgc acctgcaagc ccaagcagac cgcctgcgag cgccacgcca
    47161 cccgccagca tcgtccgtct tcgatttggg tccggcttgg ccgtctgaat gtccgtcgct
    47221 gacttgctgg gcgggggccg cgacggcacc agcaacaccc cggctcggat gtcgccgagg
    47281 attttctgca tttccgctgc ggcgtcggcc atgctctgcg gacgcgcatc gacggaacgc
    47341 gacagcatcc gcatgacaag ctgcgccatc ggggctggca cgtcgggacg gcgactggcg
    47401 atgtcttcag gatcttccgt cgccttgcgg atcaggatgt ccatcatgtt ttcgcctgcg
    47461 atcggcgctt tgcccgacag catttcgtac atgatggccc cgagcgaata gacgtcggcg
    47521 cgcgcgtccg cacctttgcc ggcggcttgt tcgggtgcca tgtattccgg cgttcccatc
    47581 gccatgcccg gcgtcgtcag cttctgctct ttcgaatctt ccagcgactt ggcaatgccg
    47641 aagtcgagca ccttgacgaa gtcgctctgt ccgtcgcggt ggatcaggaa aatgttttcc
    47701 ggtttcaggt cgcggtgaat gatgttcgcc aggtgcgccg ccgacagtgc ctgcgccacc
    47761 tgcgtaccaa tcgacaaggc atccgtcgcc ggcagccgct tttcggcttt cagccgctcg
    47821 gccaggtcag tgccttcgag cagctccatc acgaagtaaa acgaaccgtc gacggtggtg
    47881 ccgctatcgg tcacttcaat gatgttggga tggccaattc gcgacgccgc gcgtgcctcc
    47941 atgcggaagc gggcgaccac ttcgggcgtg cgtgagtaga ccggatgcag gatcttgatc
    48001 gcgacgcggc ggccaatttc ggtgtgctca gcctcgaaga cgcgacccat gccgccttcg
    48061 ccgattttgc gcaggatttt ccaacgctcg atgacggtgc caagcatttt gccggtgtcg
    48121 taggccgaca gctgctccat cacttggctc gggatttcgc cctcgcgcag cttgcctttg
    48181 tcctggccgt cgggtttttc gatttccttg aggatatcgg cccgtcgctg tgctttctgt
    48241 tccgctgctt cgcgttcgct ctcttcttgg cgtcggacgg cttgcaggcg atcgtgaacg
    48301 ttgtcgagca ggtcgtgtct ctgccgtcgc tcccgttcga tgtcttcgtc gaacaggtca
    48361 gccataaacg tccgcacgcg ctccgagtcc gtcgctacat agtcgcgatt cgacgccaaa
    48421 aatgccgtca gatcggcgcg gaactgttct cccgacgtgt atcgctcgtc cggatttttg
    48481 gccagcgact tgagcacgat cttgtcgagc atcgctggca cgcgcggcgc acgcagcgac
    48541 ggtggatcga tgtgcggatt gcgcacgcga tcaatcagct cgtgtccttc gccaccttgc
    48601 tgtgggaaaa gctgtcgccc cgtcagaagc tcccacagga tgatgcccgc cgcataaatg
    48661 tcggttcgtc cgtcgatgat ttcgccgcgc gcctgttccg gcgacatgta cgaaaccttg
    48721 ccgtagatga tgcccgccgc ggttttttcc agcttcagcg tcgagctggc caagccaaag
    48781 tcggtcagct tgacctcgcc gttgaagctc acaagtacgt tgggcggcga aacgtcgcgg
    48841 tggaccagct tgatgttgcc gaagttgtgc gcgtagtgca gaccacgcgc cagctctctc
    48901 gcgatgtgta ctgctacgtc gacggggaag gcgatgcctt ttttggcgca gcgattccac
    48961 accgcgcgca ggtccttgcc gtcgatgtaa tccatcgcca ggtacagctc ggattccacc
    49021 atgccggcgt cgaacaccgg gaccaagttg ccgtgcgaca gccgaaccac gactttggcc
    49081 tcgtcgcgga aacgatttac gtattcggga tcggccaggt gaggcagcac cgtcttgatg
    49141 acgcaaagcc gctccatgcc gcgatcaccg acgcaggcca gatagagagc gcccattccg
    49201 ccttgagcca gcggtgagag caaggtgtac cgaccgaacg gtcgcgggaa cttatcggcc
    49261 acagtcgttg cttcatccta tcacatggcc gcggtcttct ctcgacctgt cgcgccgtct
    49321 atcgtcgcga atctgcggca ggatttgtcc gacattcgga cagcgtccgg tccaccggac
    49381 aaagccagat tggaccacat tcggttctcg acgggaaatc gccatgatgt tgttggcacg
    49441 gtgtctgctt tggctcttgc gcatgaacgg tcaccacctt gccactttgc ttgtttcctc
    49501 gctcgatcgg ctgatcaccc aagtcgccga tgatccgtgt ctgtcgtcgt ccctgcaaag
    49561 ggagcgcgcg cggcttttgc tttgcatcga acaacatttg ggatcaccac cgaaggtcgt
    49621 gcgcgcgaaa accgcaatgc acagtcagcg tcggctcagc ctgcgggtgc gctgctacca
    49681 agccttggca gcgggctttt tgccaagtca gcgcttcgac gtgctgcttt tgctggcgca
    49741 cagagccaag cggcgtcgca actacagcgg ctcctcgtca gaagcattga gatcgtcttc
    49801 gccgggttct tcaccgggac gggtcgtcga ggatggcggc aaaagcggac tcggatcggc
    49861 actgcgaatg tgtttgagca atacttcgac gatctgcgct tgaagctgcc gaacctgctg
    49921 cagtcgtttg gcgcgaaatc cctcgataaa aatcgtgaag accaagaccc ccggatgcga
    49981 gccgacgtaa cccgacagca ccgacacgcc attgagtgtt ccggttttgc cacggaccaa
    50041 gccatagctc tggccgcgga aacggtagtg gatggttccg tcgatgccac cgactgcgag
    50101 cgactgcata aactccggcc ccagttcgag gctgtggtac agcctgagca gcagctttcc
    50161 gacgtcgatc ggcctgactc ggttttcgta ggtcagtccc gatccgttga ccagccgcac
    50221 tcgctcgggg cgaaaaccca gcgggaccag ccattctgtg acggcacgcg cccctttttc
    50281 caggctctgc ggcagaccat aacggaccga tccgagcgtc ttccacacca catcagcgac
    50341 gaagttgttg ctgtctttgt tgacgatgcg gcaaatttcc gagagtggct ccgatcggtg
    50401 ttcggcgaca agacgcagcg gtccaggcgg tggcggtccg attcggatgt tgccgcgaag
    50461 atcgattccg ctgtcccgca gcgccgtcga aagcgtcgcg gaaatccact cacgggcgtc
    50521 gggaatgcgc tttttgaccg tggctttgcc gcgccgactg ccgattcgtc cgcgaacctc
    50581 gatgacgagc tggccatctt tgcgatgcga atcgacgctc agcttggttt tttttccagc
    50641 gaccgttttt accgacgatc gcaaaaggaa aaatggcgac gatggctcga tggtgacgtg
    50701 ggcagctcga ccgacgctcg atgccaagac gcggacccga taggcatctc ggttgagcaa
    50761 aagggctccg ctgccttgcg cgggtgccgt cgctgcttgt tctagatcgg tgtcgttgcg
    50821 aaattctccg tcgagaatga tgtcgccgct tatgtgctgg attccacgcc gcgacaagtc
    50881 gcgaaccaag tcgacgatgt cagcgggctg gagcgacgga tcgccgctgc cttgcaaaaa
    50941 caggctgccg tcgagtgtgt cgggactgct tgccgatacc gagcgcgccg ggctccacat
    51001 cgtcgtccga aagcgatagt ccgcccccag caccgacagc gcggctgccg tcgtgaaaat
    51061 cttggtattc gatgccggat taaacgccga gtccgagttg agcgcgaaca cctcgtcgcc
    51121 ggttcgggcg tcatgcaccg tcattcccac ccgcagcttg cgcagaagcg gcccgtgcga
    51181 aagctcatgc aaccttcgcc gcagcgtctc gactttggat gaaaaattgg tcgcgccgat
    51241 gctttcgatc ggcggggaaa cggcagcaag ctctggatgg ctgcgtgcct gtgccgacgg
    51301 tgatgcgagg agcaaaccaa ccgctggcac aagctttctt agtgcccaca ggcaccagct
    51361 tggacttcgg gcggtttgta gtcgtcgcag accgatcgac attgctccta tctcgccagc
    51421 gttttttgga gatccaggga ggagaacttg tctgtctcgt tgctaaagtc caagaacaaa
    51481 aggtacgccg ctacctcgcg ctggtcaaag cttggatggg cgagcaaaaa cttcgccgca
    51541 gcgaggagct tggcgtccgg cacatccttc atgaagtccg catcggtcag gcctttttgg
    51601 tgcggcactt cgatggcgtc caggaacgcg atcagcagat ccgtcttggt ctgccgcagc
    51661 cactcaaaca tcatcacttt gcagcctggc ttgtccgcga gcaccgtcga ccacagccgc
    51721 ttcgcccact cttcgttgcg ctttttgagg ctggtttggt tcacctgccg cgttgccgac
    51781 agaccgactt ccatcatcga cgctttcatc gctgcactgc tcagcgacag aaacatctct
    51841 ttgaacttgg cctcgccaag gcttttgcag atcagatggg gtgctaggct cacaggtttc
    51901 tcctcttatc gcggcgcatt tttcgtcgac ttgccgacgg gaccagccgc cgccgcaggg
    51961 gactttatca ccgcatcttc gcggatcgtc aagaaggcga ggccgtccag gtggtctagc
    52021 ccgccatttt tttctgcgct agggtctcgt cggcggaaaa gaatgtggag tggcgtggcc
    52081 cgacgccggt tgggtatccg ccgcgccatc tttggcgcag cgaaaaccga tgtcggcatc
    52141 gcggtagtca agcggcaggc ccagccgatc gctggttcgc ggcagaccaa aaatcgaaca
    52201 gcagccacca ccgcgcagca cgcgaagttt ttcgctgggg ctgccatgca ctgccgttgc
    52261 caccgcttgt ttgtaggcgt ctttgcgata ggcatctgcg gtccattccc agacgttgcc
    52321 ggccaaatcg agcgctccgt agggcgatgc tccgcttggg tacgatccga ccgaaaccgg
    52381 ctttcctggg gcgccttctt cggcgcagcg cccatccatc gaaaagttgt cgaaattgcc
    52441 catttggcac gaaaaatcgt tgccccaggg atagcgccga ccgtcggtgc cgcgcgcagc
    52501 cttttcccac tcggtttcgc tcggcagtcg cttgccttgc caggcgcaga aagcggtcgc
    52561 ttcttgccac gaaacgtgcg tgaccggcag gttgggcgaa tcgggggtct tgcgcctggg
    52621 cagctcaggg caggctttgg ccgcgacaca ggccgcataa gctgcgacgg tcacttccat
    52681 ccgatcgatc gaaaagctgg cgacggtgat gcgctgctgc ggtttttcgt caggctcgcc
    52741 ctcgctgtcc ccgcgtacaa agggaccacc tggcaccgtg accatcgagg caaccggcgg
    52801 cggggcagcg aaaagcgaag caccgaccag cccatggaaa aacaggagca tggcggcgtt
    52861 gttaccagag cttcccattt gtgccaaacc gtttcccacg gcagcgaggc cacccaatga
    52921 gtcgtgcaga atttctggcc cggcccgaag ctgcgcgtcc gcgcgagcga cttttgtttc
    52981 tgggcgccga gcagctcagt gacgaagaac ttctggcgat cgtgcttggg tcaggtgcgg
    53041 cgcagccact tgcgcggcga ctgctcgacg aaaccggtgg tccgcagggt cttcgtcgag
    53101 ccgggcaatc ggccttgtgt tcgctgccgg gagtcggtcc ggcgcgagcc tgtcagatca
    53161 aagcggccct cgagctgggt cggcgcgcgc ttgctcccga gccgctactt ggtctgcatg
    53221 tccgttcccc gtcggatatc gcgagtgtct tgagcgtcga gctaggtcaa gccgagcaag
    53281 aggccgtcca cgtcttcgga cttgatgcgc ggcaccggat tcgctgtcgt cacgttgcgg
    53341 cagtggggca ggtcgatcgc gtcgtcgtca gtgtggccga tgtctttcgc ccgctgattc
    53401 gggaaggcat ggcggcagcg atcgtcgccc acaaccatcc aagcggagtc tccacgccga
    53461 gtgaagaaga tcagacactg acgctccggc tggcgcagac tggaacgatg ctgagcatcc
    53521 cacttttgga ccatgtgatc gtcgccaaga gtggctatta cagctttgct gatcacgggc
    53581 tgcttcgttc gccgctggca ctgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53701 nnnnncgatt tccgaaccga tttccccagc gattggcaca ggttccggac cgctgaaatg
    53761 gggaaacgaa acgcccaaaa cgaacccttg aaaccgacca cgcttgcgac taaggtgcgc
    53821 gctcgtgaat ccactggtca tcggtgttgc gggtggcacc ggctctggca agtccaccgt
    53881 ggccgccaag attgtcgagg gtctaccctc gcagagtgtt gtgatccttg atcacgacag
    53941 ctactaccgc gatcgctcgg atctgacgcc ggcgatggca gagcagctta actacgatca
    54001 tcctgactca ctcgagacag acctgctggt ggatcagcta cgtcgtctga aatcggggca
    54061 ggcgatcgac atgccgcagt atgacttcaa gacccactcg cggctgaaga cgcggcgacg
    54121 ggtggagcca gcgccggtca ttgtcgtcga aggaatcctg ctgttcgtcg aaccggatct
    54181 gcgagcgctt cttgacatca agctgttcgt cgataccgac gcggacattc ggattttgcg
    54241 acggattcgt cgggatcttg aacaccgcgg gcggacgttt cagtcgattc gcgagcagta
    54301 ctacaagacg gtgcgaccga tgcacttgca gtttgtcgag ccgtcgaagc gctgggccga
    54361 catcatcatt cccgaaggtg gcgaaaaccg agtcgcgctc gatctggtga ttggacgact
    54421 tcagcactac ctgtccgatc acgttcatcc ccagcaccca agcgaagaaa accgcgtcgc
    54481 ccactgctag ccacgtcgtc gcgtcgtgtt tccggcccgt ctgaactttg cccaagatgg
    54541 gtgggggctt ttgttcggct gtctacctgt agctcgcgag cagtggcatt ttgagcgggt
    54601 ggttcgtcgc tgcggtcgct gtccgaagcg ctgcgcgatc gagtgccacg cgatgcagtg
    54661 tcaccgtcac attgccgtct tttccctcga ccatcgcgta ctcggcatgg gccaaaacgg
    54721 ttggagcctg tccaaaggcg aactcgcgaa acggcatccc gacgctgccg gggttgacca
    54781 gcaacgtgcc gcggtgctga cgaagcatct gaagatgggt atgtccaccg gccatcaccg
    54841 tcgtgcgccg ctccccgagc atttgttcta gctcttcaac cggggtggtg gccaggatgt
    54901 tttccatatg cgatcgcggg gaaccatgaa aaagacggag ctgcgtttgt gaatctaatt
    54961 tgatatctaa atctgtttga aatgagcgaa taaaatctaa atcttgtgtt gataattcat
    55021 cgcggcacca atcgaccgct tgtttgatga
//
LOCUS       DAORGN010000008        54609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_193_length_54609_cov_6.660593, whole genome shotgun sequence.
ACCESSION   DAORGN010000008 DAORGN010000000
VERSION     DAORGN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 54609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54609
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_54609_cov_6.660593"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKL17_01810"
     CDS             <1..229
                     /locus_tag="PKL17_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43489.1"
                     /translation="AGSIFPNHKQANEGIPQTFFLFGQRILRKQATSLRALSSSERPD
                     RRCRRFQTDSPRGSFPIATTCVVLCASFVGV"
     gene            304..816
                     /locus_tag="PKL17_01815"
     CDS             304..816
                     /locus_tag="PKL17_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43490.1"
                     /translation="MQRDTPRRFFVRLQFLSGLAPSLVSDGNGQSRLTAFVPLDVELA
                     TRLDGVLSLAAAVQTELAPFSLPNCPDGPPLRPHAASSLVGARIDLNNNRDGSWWSPW
                     VALRMGIGGQIVSDGSCASSVRLFPAFSPRLGADLWMGKSAASFSVGYDYLPTGSLVT
                     LQIGLSLRVD"
     gene            complement(823..2298)
                     /locus_tag="PKL17_01820"
     CDS             complement(823..2298)
                     /locus_tag="PKL17_01820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK43491.1"
                     /translation="MNSYSLLSALVMGSLLLAPPVGAAPVVVKSDWRQIKPPPLHAMK
                     PQQPVRVVLDNGIVLLLQQDKELPLMTMNVMIRGGVKDEPFDKTGLSDIFAESWRSGG
                     TKSRTGDQIDDFLALRAAQIETGSEATRTTLTTHCLKKDFADVLGLAVELLREPEFRQ
                     DKIEIAKRQVYTAISRRNDESSEIAGRESTKLAYGKDHPLARQEEYATIAGITRDDLL
                     LWHRRFVHSGNLIVGISGDFDPDELLALLKQKLATLQQSQKVTLPELGFVPTRPGLYF
                     VPKREVNQSSIHLVQLGTTRKNPDHYALEVMNEILGGGFWARLFSNIRTKQGLAYNVG
                     GGVGLGWEFPSLYKVAMSTKSQTTGKSITALFEQVQGMLMNPPTPDELQKAKDAILNS
                     FVFKFDSKAKILSDRMRLEFAGYPADYTEKYADQIQKVTLEDVKRVAQKYLNPGGFAV
                     LVVGNPDEIGDQLTQLKHLGPVQTVDVTIPPPPPRQNPAQK"
     gene            complement(2301..3830)
                     /locus_tag="PKL17_01825"
     CDS             complement(2301..3830)
                     /locus_tag="PKL17_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK43492.1"
                     /translation="MRRHLLGFALAIGLSSGMVPTGLGSGVAHAQDLASFEKRTQVKQ
                     LGNGLTVIVVERPEAPVFSYFTHVDVGGAQEAMGQTGLAHMFEHMAFKGTDKIGTKNF
                     VAEKVLIDKVEKAYLAFDAERRKPSGSDPKKVEALEKAWRDAIAEASKQVVANEFGEL
                     VDRNGGVGLNAFTNSDETGYFYSLPSNRLELWAYLESERSKRPVMREFYTERDVVTEE
                     RRMRTDSRPIGRMLEQLLAAAFTAHPYGRPVVGWPADLATFSATDALKFWETYYTPAN
                     MAIAIVGDVKASEAMPMLESYFGRLPKGPKPAPLSTVEPKQFAERQVILRETAQPFYA
                     EGYHRPDVFDKDDAVYEAIADLMSTGRTARLYRSLVRDKKIAAASGGFSGMPGRKYPH
                     LFAFYAVPTPGVTPPQVRTAIHEELERLKNSLVTDEELQMVRARAKMRLLTSLGSNQA
                     LAIAMASAQQRYGDWREVFRRVDRIDKVTKEDIQRIARAVFIESNRTAVWIESTAQGG
                     K"
     gene            4052..5617
                     /locus_tag="PKL17_01830"
     CDS             4052..5617
                     /locus_tag="PKL17_01830"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNK43493.1"
                     /translation="MKTRLTLFSLFLVAACGNADPSPNQNPVIGESAVTSRQPSEIGQ
                     ALQIAATQFAIPQSVLQATAYAQTRLFFVPGQAGEASGDEPQYGIFALRSAAIRKGAQ
                     LAGISDHDAQTTVIGNVQAAAALLRSYADEAGIDRTDLAAWAGPIARLSALPTEQAQA
                     DFIHNVVYKTLREGLPPEIALGHGLSDAPHPDLVPQFAALVDDSLPYAGVRYSGSVWK
                     PAPSSNFTVGRSAAIDLLVIHTCSGGYSGCVGWLQTPYPTNPYKTSAHYVVKEDGTEI
                     AALVDESNTAHHVGASWEGRPTNPRSVGIEHGGFSYQGTNKWTEGQVSASAKLSCDVV
                     KRNRIPRDRNHIIGHYQPDPVNRADDPGRDFPWADYMNRISRCVGGSTAIVVDSNQAN
                     NDASGKIDTPSSSWTSSTNVAGYYGSGYYVAPTAAVSDAVYFSFYLTEAANREVFAWW
                     TAASDRSTETPFVIFDAAGNKLATVKKNQQDNGSQWVSLGRYNFTVGWNQVAISRWAT
                     AGSVVVADAIKVE"
     gene            5623..6612
                     /locus_tag="PKL17_01835"
     CDS             5623..6612
                     /locus_tag="PKL17_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43494.1"
                     /translation="MLAWLAAAAALSFISERDGNREVYVIAADGSGERRITRSGDADY
                     NGPITPDGKYLLVTSVSELQHEKQQRFFLYPTAGGVPKPLGPIRSLLRHPTFSRDGRF
                     LFFEGSSTDLREIFRLQIDGQKLIPITKNREGNFQPCLSPTGDRLAFVSSRDQVAELY
                     ASSPTGGPARRLTQTERDEWQPQFFPDGKWLLFGSDRDGADRLYRQPVDGGTAKRLSP
                     EGLDPFVVEESASISPDGSAVVYVHRQRDRKDKLCLVDVATGKRTTLFESQADPATEP
                     VFSPDGTLVAFTLGLGLHSQIFVVARDGTKPRRLTQAKGPNWHPLWLSRPSAR"
     gene            complement(6599..8200)
                     /locus_tag="PKL17_01840"
     CDS             complement(6599..8200)
                     /locus_tag="PKL17_01840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43495.1"
                     /translation="MRPVFTPPSEVAEYRIVRLLGRGAMGQVYLAHDTLLDRPVAIKF
                     IAADPNSTVRERFLVEARAIARLTHPNVVSVYRVGELGGRPYLVSEFVRGHSLAELAK
                     PVSVEKGLQIGLGLCAGLKAAHASGVLHRDVKPANAILAESGDAKLLDFGLAKLVSDP
                     LRSDDGESSQPAIPAADVISGDEATLTPEALAKSRTTSTSGRPSDVTLSAAEEAAPAE
                     QPTPFDEPLSALREPTPVSLTKTGALVGTPRYMAPEIFAGEAATARSDVYSLGALLFE
                     LLSGRAPFVEPDLARLSIAVRLREAPPLRGLLPDHPLSDALAEVIDRCLARDPKLRYA
                     DGGELLRALQSVAVSAPPVAEAAVPARPSSSVDPNANVAHAPSTRNRWVWLSVAVLAT
                     GAGLALRWGVSPTTNRPPDFGTGPLVETPRLDLAVSSRAASFIDLRRADDLSSLPDLA
                     PSVRSDVNVVSTPEKVQSLRRGPAASKKNSAKVQEPIVVAKPENRAAAPAPAAPVDSA
                     PAAPPSTAPAPSKPQVDKVEIPIER"
     gene            8301..9551
                     /gene="glyA"
                     /locus_tag="PKL17_01845"
     CDS             8301..9551
                     /gene="glyA"
                     /locus_tag="PKL17_01845"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNK43496.1"
                     /translation="MLRDTLRRVDPEIFSLLEQEDQRQRDTIRLIPSENYTSAAVLEA
                     LSSALSNKYSEGYPGKRYYEGQQFIDQVEQLCIGRAKTLFGAEHANVQPYSGSPANLA
                     VYLAFCKPGDTIMGLGLPAGGHLTHGWNVSISGKYFVSVPYGVRKDTGRVDLNEVAEL
                     ARKHKPKVLFCGGTAVPRTIDFPGFAQIAQEVGAVLVADIAHISGLVAGGAHPTPVGH
                     AEVVTTTTHKTLRGPRGGMILCNAKHAQALDRAVFPGLQGGPHNATVAALAVALKEAQ
                     SEDFRRYAHQIVKNAKALATALLARGYELVSGGTDNHLILIDLTNKGIAGKRAAQALD
                     RAGLETNYNSVPFDPRKPFDPSGIRIGTPSVTSRGMLESDMEQVAAWFDRAIEAEKQS
                     DAQAITRIAGEVREFLLRFPAPGL"
     gene            9572..11332
                     /locus_tag="PKL17_01850"
     CDS             9572..11332
                     /locus_tag="PKL17_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43497.1"
                     /translation="MTRGTARAAVGLLLFFCGQAAHAEPAQAPTAAQAPTAAQAPTAA
                     QAPAAAVQTEVPHKLTPVLSDDELGKLQALMLSPQSAVRLQAAQRIATADIGDGDGEA
                     RYATWLRKELVGQTQVMQKLIHAIWGQYPNPDYPRGPGKDPPMWMIRPEPPIPPGTAK
                     KQKPKPHDPEAVDWLLALSQLDPAQEPILQGFPVEEANKTRAELLLKVALLRALSQAG
                     QRGSREAVHPVFEQAFVRDGVLRDECGRMIRAMGSPAIPGLVRIYNNRSRANYKMRRY
                     ASYQLDRMDRLRPSKAIVSASDDLVRADIIHAYGEVLAIDAVEAILEQVSARSRRVRR
                     EARWAWLRYVDGPPPPPPPKRKRKLPGGKEEAEEKEDYLSYRELATLALRRTHKAVFG
                     EEPSDKLSAKQLTDELFAYYDKQEEALFAKLFASAEEHLSTGQPQLAVSEFGWILANQ
                     PDHPRRVEMALAFRKYGEQVKAEAEQKNDDGLRSQAVGWLRHAVLLSSNQPDIAKLRA
                     QIHLLDGKLALKQGGDGRSDFAAALHEDPSLSEARTYLHRVVTQPQRRIDWLRWLCLS
                     LLASGLLLLGLWRRQNASLK"
     gene            11413..11628
                     /locus_tag="PKL17_01855"
     CDS             11413..11628
                     /locus_tag="PKL17_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43498.1"
                     /translation="MNKVVSFAMLALLALAAACGPKAQAGGGTTVAPPVGGAPASGTS
                     GAAPSGSGGTDAAPPMNANPGSAPAAP"
     gene            complement(11648..13081)
                     /locus_tag="PKL17_01860"
     CDS             complement(11648..13081)
                     /locus_tag="PKL17_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNK43499.1"
                     /translation="MLAVSYTAIPYCLRSGALSGGAAPWVAVSAFLVVLGAAFMYSPP
                     IRRAVVTLVQLLVYFRRYLLRRFRGEPQLAGPVELRELFVRLGPTYIKFGQLIASSNG
                     LFPERYVKEFQKCLDRVPPEPWELVRQTLTLALGKPPEQVFTTIDTKPLASASIAQVY
                     AVTLVENGETHDLVVKVQRQHLPELVAADVSMLSFCADVLAMLPGVRAANPQAVVAQF
                     QRNIAEELDFLGEASRMEEFNQIMRDLGRSDVVAPRPLRALCRRDVLTMERLHGVRVD
                     DLDGLLRIGITREQTEARLIDGMHAWFSCMLRYGFFHGDVHAGNLMALRDGRLGFLDF
                     GIIGRLDRTRRKQIASYLLGFATQDFMQLAKTMAAMGAVARKVSDEEWTTFAADLKVA
                     YQPFLRGTLGGIDYSEVITNLLKVAERHGAHMPSEFVLITRQLLYFDRYAKLLAPSLN
                     LFADPRLLMTVGAEVMSLGGLADESSP"
     gene            complement(13215..15263)
                     /locus_tag="PKL17_01865"
     CDS             complement(13215..15263)
                     /locus_tag="PKL17_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK43500.1"
                     /translation="MSLASLWSSPSAHAQDVKAGFQVNRYEPTAAGEWSFWVDHPWYS
                     STRYFAAGITLNYAHNPLVFGRTDSTGSFSQTLSVIEHQLIGHVDLAGSFLDRVLITA
                     TMPIVLLERGTPAAGAAPASGVVISDPRVGLWVRLFGQPYRSAVSMSIGANAWIPLRA
                     FADGSAAVSNGSSDAGIRIMPKIALGGLSHHVMWSFGAGFMYRTPAKIGNATVSDVGS
                     TVGSELQIGAAIAYADTQRRFAIGPEAIFSTVVLGTGSVKPFGSDFTSLEVLLGFHYN
                     IAKILQLGVGGGLGLLREPGTPDGRVLLRLAYAPWKDGDDDRDKDGISDKEDACPDNA
                     GIRTDDPSTHGCPDRDRDSVVDKIDICPDVHKGKTPDPKRLGCPVGDRDKDGVLDSDD
                     ICPDDPKGATPDPNRLGCPAGDRDKDGVLDPQDLCPDVPKGDTPDPSRLGCPAGDKDN
                     DGVPDPKDLCPDVPAGHKPDPAKLGCPLPDRDRDTVVDPEDACPDQPGAPSPDPKKNG
                     CPGLVRIENGQIAIMEPVFFATNKDTILKKSFPVLNHVVDALKASPFIKKIEVGGHTD
                     DRGKADYNRDLSGRRANSVVKYLVDHGIAADKLTAKGYGPDKPIADNKDKKGRERNRR
                     VEFVILDPAQPQNVKTKSAAEVQVPDSPDQSDGKGKADKKGKADKKAAKPAKKAKKAS
                     "
     gene            complement(15389..21445)
                     /locus_tag="PKL17_01870"
     CDS             complement(15389..21445)
                     /locus_tag="PKL17_01870"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNK43501.1"
                     /translation="MSTEPSDKPASESPSSDKPATENATNAAADGNAGLGGFPAAHAG
                     DQPGVIRRIGRGLFGQVSWQPPPWLAAIGRGVASGGRWAQNHRSAALLVVLMICAFGG
                     AAYLGNRWLEKQPKPIEMSVRVDAPEATRLEDNAKPDSLRILFGGSAAPIDKVKKPVT
                     VGVELSPPLQGSWRWDSDRVLTFVPKEDWPVGEEFSVKLARKGLLADDVRLTEYSLNF
                     QSAPLTGRFTAQEFYQDPQDPTQKKVVATIAFTHPVDKAEFEKRLEVELVTKKDGAQS
                     VKPYRFQVTFDRFAATAYVHSDPVQIPDHDSMMMVRVQKGVKSSRGGKPTKEPIGTSV
                     TIPGLYDFLRVSEASLTLVNNAQMEPEPVLVVQTNTGVTESEMKNALSVVVLPKRPPN
                     DKGSSKEQYGFSLSEIGPDVLKLARPVKVEQIPNEHEFSETHSFRYFAEVGEVLYIKI
                     NKGMRSAGGYVLGRPSEHILTVPEFPKELKVMQTGALLAMSGERKVPLFGRDIAAVRV
                     EIGRVLPNQLQHLISQNTGSFQNPDFSNYRFTPENITERFTDEVTMPTVAHGKAQYAA
                     VNLGKYFDAKGQSRLGLFFLKVESWDPDKKETTGQSDQRLILLTDLGVLVKDNVDGTH
                     DVFVQSLKTGEPIKGATVEVLGKNGVAIQSETSDAEGHVSFPKLSDFVREQAPLAYQV
                     SKGGDLSFLPYGRHDRYLDFSRFPIDGIHDSEKPQGLQAYLFSDRGIYRPGDEIRVGL
                     IVKSQDWKQKLGGVPLELSIDDARGVTVRREKFKLTDAGFEEIRHQTLEASATGTWNV
                     NVHIVKDGQTSSLLGSVAVRVQEFLPDRLKISAKLSREALEGWVSPDGLKARVQLTNL
                     FGTPAENRRIKATLMLSPTLPSFSKFRDYQFFDPMQAKEPLTENLEDGETSEHGESEF
                     QLPLSRFSSATYRLRFIAQGFEAEGGRSVAAETQVTVSPMAYLLGYKTDGDLGYVSKA
                     SKRNVHLIAVGPQGTSVAQSGVRTMLFERRYLSVLIRQDNGTYKYESVKKELLVEEKK
                     LSIPAGGLTYPLPTTQAGDYFLVFKNDKDQVLNRIEFSIAGEGNLTRSLEKNAELQLK
                     LKNPDLSAGEELEIQIKAPYTGAGLITIERDRVRSYKWFKTKHNSSIQKITIPDDMEG
                     NGYVSVAFIRGIDSTDVFMSPLSYGVAPFSISREKRTAKISVTTPDLVKPGDAFKLRY
                     HTDRPTKIVLWAVDEGILQVAKYQTPDPLSYFFQKRALEVKTAQILDLILPEFSRLLK
                     AAPGGDGDGGAMKNLNPFKRKRDKPVAYWSGIIDADTDDKEVVYQVPDSFNGTLRVMA
                     VAVSADAVGVWSKKALVRGDFVLSPNVPMVVAPGDEFEVSVGVSNNVAGSGANAKVNV
                     QLQTSKHLQVLADASQTIKIGENHESVVRYKLKATQVLGSGNLTFTASLGGKSGKYSI
                     DLSVRPAAPLYTTTQVGHLPPGGGRTQLQVPRELFAEYRVLQAGIAPLPLGLSQGLAQ
                     YLEKFPHGCTEQQVSQAMPALIGRGRPEFGIKTEAADVSFQGILQTLRSRQNEDGGFG
                     LWANPNQVNDFASVYATHFLLEAKERGLPVPPELLKKSFEFLNQIVARETGSLSEERV
                     RAYAVYLLTRSGVVTTQAAMSVRKRLENQYKNWGEDLAGIYLAATLQLLKQTRDAQRI
                     MASVQLGQVKDSASDYQNYYDPLIRDAQALYIMARHFPDKVKSLPADALLTIVDPIAK
                     GRYNTLSSAYLILALDTYASLAQDTTDVSQLSIIEVNSGGQQKPLQLPTGLLPQVVFS
                     QDARKLLVSSSSPLRTFYQVTQSGYDLRVSTDVVKQKLEVLREIVGSDGKPIKEVKLG
                     DEAEVHIKLRSLGKETQLWNLALVDLLPGGFEVVVTPPAASEESSESSDEEGGGSHDD
                     GDDEGRHEGASEGGEGEEGDHGSSDSSSRSERGARKTSAPPAALPIAKDGSTWTPEYG
                     DVREDRVVLYGSVGNDLQEFVYGIRATNVGTFAVPPLQGESMYDRSVIARSAGNKIRV
                     VK"
     gene            complement(21524..24271)
                     /locus_tag="PKL17_01875"
     CDS             complement(21524..24271)
                     /locus_tag="PKL17_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK43502.1"
                     /translation="MSSLGFNREVKLASGSWAIAAGVGLCVWCAGPAQVLAASSSQAA
                     SPPVSVSVDLDGDGRMETLSLDEAGTLAIAAGTGAGSRLALLLGDRVPRLSDGRLTIE
                     KLRGGETLVVATGLRGKGQRVALWARWRAGKLEPVYSGPIGPVGSDGEYAVVVEVSDG
                     ELFRYQTAPSVERCDGERRLFLERFFPDGDGKWQPAPDRLLAKPEKVAPLSTSPTGPS
                     EFVNQPIGMYRMWAVSRQAGIERADLLSPPREVDDGQPQTVWRGPHDATGTFFTWRAE
                     SAGRSLRAVRIVPAPSAQGIMPAQLVLTLSSKQSFRIPTAGVAKPVWIVLPQPVPTDC
                     VSLTIEQPGTKAGQHSALGDVNLYSDLDGGDAVALLIGQLGSTDTRSAEAAERTLRGR
                     LESMRPEQIESLLQSLAAAEKTAKGSARRRIQGLLGTLAERSSVMGGKNQAALIDVLM
                     ATLQSAEVEERPALFSALTAASRSREPLELAVGKLELLLLDGKRSSLLRAEAAGWLCE
                     HSAWPYPLQLGQKLTRETGLRTAFAAAMGRALHCVGPQDPRVATALSEYRRTDVDPAW
                     LVLLGEGLLAAQSGCPNDDSRQQLAEKLGGFWSQLASQASAETRFALRYRLLAVLSKL
                     DLPTVDPLVMTVLQNETEPDLRKLAARALGRSRTLDLDGVKRAVSDPDPGVRTALLVG
                     LFGRSSVSGQSEALSGLLSPLLARDSWPMVRRAAAEVLGSLCQPGGTAQASLEQAVAD
                     AEESVAAASLAGLARCVGAQGISRYQSLLLDEKTMPSVRGQACVLVARFGLGTNSAAS
                     AHRAIGDGLSDLLDDPHGADRSLVAALLCIRALGEHGDDVDLALLMGRLDRDAPLALR
                     RGAADSLLRICQRQKTPLGKNDRQSLGELLKRAGDPSDALLHGLQNKLQRACGPLSSV
                     R"
     gene            24467..24760
                     /gene="yajC"
                     /locus_tag="PKL17_01880"
     CDS             24467..24760
                     /gene="yajC"
                     /locus_tag="PKL17_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNK43503.1"
                     /translation="MSNMLLPVFLIGIFYFILIRPQQKRQRELEDWRKQIKKGDEIIT
                     SGGLWARVSSSEDKSRYVTVELQDKVRVKVLREHIISKAPPADVSVAAADEAK"
     gene            24857..26677
                     /gene="secD"
                     /locus_tag="PKL17_01885"
     CDS             24857..26677
                     /gene="secD"
                     /locus_tag="PKL17_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNK43504.1"
                     /translation="MERSFYYRVATYSLITILSVMVLLPSLADWTHKTDRLPDWFKKA
                     FTRKISLGLDLQGGLHLVYEVQVDKAVSDKADRLAAQIEEKLAKDKKVKGARVVREGD
                     GAEARLRVNFQNKSDAKKLEGNFELEFAKYLTRDGQNEQTGETVYKMDSDYIDELRSY
                     AVSQGVETIKNRVDKLGVSEPVVQRKQNDIIVELAGLSQADFERVKSLIGKTAQLEFK
                     MADDGAEYMQKLSAHAESKKAQYPGLDVGFDAWQQKDTGQQHTDVYLKAKDKTVMEQF
                     LKELPPELAVPPDHQILFEQTARRNDDGEETQDKLWRSYYLFRRAELTGEYLTDAEVQ
                     FDPNTGRPEVAITFDSEGAGLFEKVSGSNIGRRMAIILDEKVSSAPTIEGRIGGGRAR
                     ITLGGFSDPYQLQQEAKDLVAVLRTGALPAPLRKTFETQVGATLGPEAVSKARTSMII
                     GTLAVVLFMLIYYRAAGIIADIAMVFNVIYLLAIMALIQATLTLPGIAAVVLTIGMAV
                     DANIIFYERIREELRSGKTPRQAVENGFGRAFWTVFDAHVTNLVAGIVLYSYGTGPIR
                     GFAVSLMAGIVCNLFTSVWLSRVMFDFMVGRNKKRATLSI"
     gene            26685..27893
                     /gene="secF"
                     /locus_tag="PKL17_01890"
     CDS             26685..27893
                     /gene="secF"
                     /locus_tag="PKL17_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNK43505.1"
                     /translation="MSQQFFELIRPDHNFDFVGKTKPLLLSSAVLIVLSFLMLPLNMV
                     IPGRGHALNFAIDFRGGTEMRVDFSREISPSEVRDAMETGGYKDSEVVPLPEFKNAFL
                     VRFPAVSALQPEQVKQAEAALRGKLGQEALRKFEFSDGGDKAYVKLDKSKSVEPAQID
                     EILRSVNIKSNQVQRFGRPEDNTFEVVLVGLDREVRAALDSKLGQGAVSAIPSVGSVG
                     AKAGKELRDNGIISLLAAIGFIMLYILVRFDFRYGPGTVIALLHDAIFVLGAFALTYK
                     EFSLTTIAAILTVIGYSMNDTIVVFDRIRENASKMRDKKFPMIVNLSVNETLSRTILT
                     SATVFFVTLAMNIWGTGVIRDFAFAMNVGVIVGTYSSIFIASPILIWLNEKSMQSQKN
                     PRRSSAVSPV"
     gene            27922..28776
                     /locus_tag="PKL17_01895"
     CDS             27922..28776
                     /locus_tag="PKL17_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK43506.1"
                     /translation="MSPRSLPSLSEFQHSGQFVDFRGHRIFVRDAGHGPPLLCLHGFP
                     TSSYDFARIVPELVAHRRVVLFDFLGFGFSDKPQHHPYSLFEQADIAQAVAKELSLGK
                     LTLLAHDMGSSVALELLRRSTPEIERLVLLNGSVFLRHYRPLLSQRLLLHKQVGPLLT
                     RLGLINRRVFHAQFAKLFPDRPSDEELDLFWQLIVHNGGQRNYHLLIRYLDERHQHEL
                     TWLDALTAHRAPLTVIWGQRDPVSVPAIAKEVLLRRPDATYVPLYDIGHYPQWEDPQT
                     VARIVLGL"
     gene            complement(28780..29913)
                     /gene="wecB"
                     /locus_tag="PKL17_01900"
     CDS             complement(28780..29913)
                     /gene="wecB"
                     /locus_tag="PKL17_01900"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNK43507.1"
                     /translation="MMTKNPLTVCTVLGTRPEIIKLAPLIPLLDEQPGMRQILLHTGQ
                     HYSFEMDALFFAELGLRPVDVQLAVGSQTHAAQTAAILVGMEQAIQRERIGLVLVLGD
                     TNSTLGAALSAAKLAVPVVHIEAGCRSFVRTMPEEINRVLVDHCAAQLLAPNEASKTQ
                     LLSEGISAADITVVGSTAIDAARAFANKAQHRTVVIDALLSLGLSPQTPFLACTVHRA
                     ENTTPDVLPGVLRGLGRLAERYPILWPVHPRTQRIIDERKLVVPPRIRLLPPVGYLDM
                     LALLQQARALCTDSGGLQEESYALGTPVLVLRNETEWMYLVDAGCAKLIGNREADIGQ
                     RGLACLDEHEVLAMRAAAQALTLRLADGGAAQRIRDAIARRWL"
     gene            complement(29910..30665)
                     /locus_tag="PKL17_01905"
     CDS             complement(29910..30665)
                     /locus_tag="PKL17_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK43508.1"
                     /translation="MSELSSRETLSIVIPCFNEEDALPQLCARLPAVLDKLASRYADV
                     KVLLVDDGSTDATPRLLRECFGSDPRFCVLSHEQNRGLGAALRTGFAHSRSDVVVTTD
                     ADGTYEFGEILGLCALLLPDVDVVTASPYHPEGGVDGVPAYRLLLSQGASLCYRILLE
                     RNLHTYTAMFRAYRRKVLDQVQFSSPGYLAMAEILSESLLAGFRVVEFPTVLHVRKYG
                     QSKARVARILADHLRFQAGLLPRAMMRKVAQLG"
     gene            complement(30730..31923)
                     /locus_tag="PKL17_01910"
     CDS             complement(30730..31923)
                     /locus_tag="PKL17_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43509.1"
                     /translation="MLVRFAVENFLSFGPRWQLDLRAPEGTESAPLGPILPDGFRVLP
                     LALIVGDAATGKSNLLKALAQLRQLALHGQKPGQRASLPVCRLASPPRSDVAFAIEVF
                     AQDALYRYELALRPEQVDAESLSVCVRGEPEQLVFRRERKNPILQLTDVKVGPGARSE
                     RGLWESLAVHLGAEQPLFAAALAEGSQVAAPFGAWLSEQLQLIRPEPRVTGLSARAAH
                     HPAFAEKLAELLRGSGLPVTHVAARKTPIGPDYFENEEEQRQVVSALLGYPDAFVQTP
                     DGELIAEREGNFVDLFLTSLQVGLRGPSGRESDFSPSELSETALRLLHLSPLAIPPGQ
                     SQPPVFFVDDLGRGLAVDTVNRLLSSTDRPATSQVIATATADSFLLRMVPEQAQRVLP
                     ALVPK"
     gene            32019..32396
                     /locus_tag="PKL17_01915"
     CDS             32019..32396
                     /locus_tag="PKL17_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNK43510.1"
                     /translation="MRKLLWSAALSLAVASSACSNETAVLSLDPPQGTYSGGEEIVIK
                     GRNFPTGRGGVSVLFGRHAATNVILESASAIRVTSPAGDRNTKADIVVMFDDGRAYQL
                     KDAFQYLDATDNSKVMKHFGNKK"
     gene            32430..32765
                     /gene="gatC"
                     /locus_tag="PKL17_01920"
     CDS             32430..32765
                     /gene="gatC"
                     /locus_tag="PKL17_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNK43511.1"
                     /translation="MSSAMDRLISADEIRVTAEMANLTLPEQEIPKLVDELSAILGYA
                     QSLLAVDVTGVPPMTHAVPSHCPLRGDVVLAHDSTDSAMRNAPAKEASYFAVPAVFAG
                     GDDGFDAEG"
     gene            32769..34325
                     /gene="gatA"
                     /locus_tag="PKL17_01925"
     CDS             32769..34325
                     /gene="gatA"
                     /locus_tag="PKL17_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNK43512.1"
                     /translation="MTAQQSVGDSFGSALELAQKVRSREVSAVEVTRSYLQRIRRFDE
                     ALGCFLCVDEKGALAAAQAVDEKVRDGADEELVLAGVPVGIKDMLCTEGLATTAGSKI
                     LAGFVPPYDATVVAKLRDAGAIIIGKCNLDEFAMGSSNENSAYKPCKNPWDLTRVPGG
                     SSGGSAAAVAASLCPISLGTDTGGSIRQPAALCGITGMKPTYGRVSRYGVIAFASSLD
                     QVGPLGTSAIDVAQTLQVIGGHDSHDATSLAMEQPDYLAACQRPLVDKGRRVRIGVPK
                     EYFAKGLDPRIERCVRDAMARLERDGAELVDVSLPLTEHAVAIYYLVATAEASSNLSR
                     YDGVRYGLRVTAKEGEPLSGPSALVEMYKRTRAAGFGPEVRRRVMLGTYVLRSGYYDA
                     YYRRASQVRTLVARDFAAAFARCDVLLTPTSPVLPFRLGERLHDPLAMYLSDIYTIGC
                     NLAGLPGLSMPCGFVCEPGDEKPLPVGLQILGPALHDAEVLRVAAAYQRLTDWHTRRP
                     TAFLAKKEPA"
     gene            34327..35817
                     /gene="gatB"
                     /locus_tag="PKL17_01930"
     CDS             34327..35817
                     /gene="gatB"
                     /locus_tag="PKL17_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNK43513.1"
                     /translation="MASPVEYEPIIGLEVHVQLSTLSKAFCGAKAAFGGAPNSYIDPY
                     TLGLPGALPVLNHRAVEFALRMGLACQCDIALRSELSRKHYFYPDSPKGYQISQFDKP
                     ICENGHVEFVLRGEKKRVGLQRIHIEEDAGKNLHLPGGRTSLVDFNRAGVPLIEVVSL
                     PEIRSAEEAAEYLRALRQLVRFLGISDGNMEEGSLRCDANVSIRPRGQTTLGQRTELK
                     NINSFKYVQHAIEHEILRQIDVVQSGGKVVMETRGWDAARGTSRSQRKKEQAHDYRYM
                     PDPDLPPLHIDPLWLDSIRAALPATPMQRRDRYIDRLGLSPYDASVLTAERELCDFFD
                     AALVASGVSTDSESVSEAQCSRAKLCANWLSSELLGLLNKESRDITASPVSAESLGEL
                     VRYISDDIISGKQGKEVFSRMYESRETPTDIIERLGLRQITDEAVITAACRKVVADPA
                     LAKQIEKYKQNPKLLGFFVGKALAETQGKAKPDLLSTIMQRVLSEK"
     gene            35820..36860
                     /gene="mtnA"
                     /locus_tag="PKL17_01935"
     CDS             35820..36860
                     /gene="mtnA"
                     /locus_tag="PKL17_01935"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNK43514.1"
                     /translation="MSASLHPTLLSPLRFDKGLCELLLIDQLALPGEEIWRRYSDYQA
                     VADAIRNMVVRGAPAIGIAAAFGVVLGVKAGEPLDQVIATLRSTRPTAVNLFWALDRM
                     QRRAAELSILSHHKQVEGLCTEAQAIWDEDVAMCLRMAEHGAKLLPPGNVLTHCNAGG
                     LATGGYGTAVGVIRSAFALGHIAGVFADETRPFLQGARLTAYEFQKAGVPVTVICDNM
                     AGHLMARGEIQSVIVGADRIAKNGDVANKIGTYSLAVLAKAHGIPFFVAAPRSTLDPK
                     TASGRDIPIEERSTEEVVKIGPVRLAPVGVLARHPAFDVTPAHLVTAIITDAGIARAP
                     YDTSLSALLLAT"
     gene            37056..37532
                     /locus_tag="PKL17_01940"
     CDS             37056..37532
                     /locus_tag="PKL17_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43515.1"
                     /translation="MDGDVPAKVECLSCHKQHKFKAAPPGTKVAKAAAGGTKAPRAKS
                     ATRPAAHSVPVVTGPNPLDTILQTRGIGSARSYSPAERYAVGELVSHPNFGIGAVTAT
                     PSPGKMSVLFRDTTRLLLHERTPVAVAPVAKLGPPPRREESPTGPADRPPKVKPLL"
     gene            37593..39338
                     /locus_tag="PKL17_01945"
     CDS             37593..39338
                     /locus_tag="PKL17_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43516.1"
                     /translation="MSIATRVTLLTVLLVTLALGVYGTLSMRGRRAERTAELERQTWL
                     FGSALKVALEAAAQDGLYEDLHGLIGRMQAAERTIEVTYQDLLQPGEDAVFPPGSSAR
                     LVRQPNPDAGREERRRRVEITRQPYGEHIYISGREVYAYSLPIVDNKHRMVAIIDLQR
                     DKSELEQEMLRATRDVVLTLGGLAALLAILVSLTVRQEFSAPLLRLVGGIDEVTRGDY
                     TGAILRERNDEVGLLADRFNEMTQSLREARNEILAGVDAKLQLEQRLRHSDKLATIGQ
                     LAANIAHEVGTPLNVIGGRARVMSRRAEDPTAVQKNADIIATQAERITKIIQQLLDYA
                     RRKAPARSDVDLRKVCLSTLDFLEVQLTTRHVAAQLHPFAVPTPPQPESQIERAVRGD
                     ESLGTLKPPAQPIVVGDSDQLQQVCLNLCINAIQAMPEGGALDLSLEGIVRRKPGLDK
                     APPGAYVMLAVADTGIGIDEADLSRIFEPFYSTKTDGKTGKDGPSGSGLGLSVSAGIV
                     KDLDGWIECERRPTGGTIFRVFLPAPEREEPNTRPQRSRPPARQKQQSTVPESEGNSF
                     EAAPADVLSQIETEH"
     gene            39338..40873
                     /locus_tag="PKL17_01950"
     CDS             39338..40873
                     /locus_tag="PKL17_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK43517.1"
                     /translation="MAQPPKTTSKLPQVPLAAGKSGPRSQPPPLPSDTSSDRASRHTI
                     LVVEDDAAMRDLLEDSLRDEGYRVETAAGGHAGVARVQKGGIDLVVTDVRMPDLDGLD
                     MLREIKSAPLEQPPHVIVITAFGSIDTAIKAVRLGAHDYITKPFNMETLFLAVEKALE
                     ERGLRQEVSRLRREVERPYQFDNIIGRSAPMQEVFGLIRRLSGSSVNVLITGESGTGK
                     ELVARAIHYHSSRAKQPFVAVNCAAIPDQLLESELFGYKRGAFTDARSDRSGLFVEAD
                     QGTLFLDEIGDLSPALQAKLLRVLAEREIRPLGSSRPEKVDVRVISATHKDLSQGMRA
                     GSFREDLYYRLNVLQIQLPPLRDRAEDILPLADHFLAKARERGGKRVTAIHPQATKLL
                     LSHSWPGNVRELENSIERAVALCESSQVMAHDLPPQLREKRREDVIAYALSRNMTLEE
                     LEREYILRVMKEESGNKTRAAQRLGLDRKTLYRKLEEAGKQTAGDGDGDGETDNGSAD
                     DHD"
     gene            40848..42638
                     /locus_tag="PKL17_01955"
     CDS             40848..42638
                     /locus_tag="PKL17_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNK43518.1"
                     /translation="MAQPTTTTSDPLPRGFRRLILAYYVTIRVILSYYFLRFRLRIAG
                     PDAASRLLMDAHRKNARRIQVAIERLGGLFIKVGQLISIMTNVLPEPFRAQLETLQDR
                     VPARPYADIEQRFTEEFAGRRPSEVFASFSPEPVASASIGQVHRARTQDGHDVAVKVQ
                     YPDINEIVRIDLAALGRIFWLLHWIAPAHGLDAVFVEIRAMVMQELDFSAEAHNLSRI
                     SENFGRYKPPLPVDFPKVIAELSTPRVLTTTWIEGVKVSNLSRLDELGVDRDKLARTV
                     VTAYCQQIFRDGLYHADPHPGNLLVLPAPKPSGEGDAAHANDVASVPTPWLDLGGAKL
                     VFLDFGAVAELSPTMRRGIVDVLQAALTRDTQRLIVAMKEMGFIARGADPQIFERVVD
                     FFHDKFQAEFKLDSLSLSEVKLSPERALADLADLRKLDVNLRDLMEHFHVPKEWILLE
                     RTLLLLLGLCTDLSPELNPMQVIRPYVEEMVLGQDRDWSKLLVETTRDVVTQAAQLPG
                     ELRKFLNRAQRGQIEVRFRGQEDTAILIHAVARQLIVTALGITAFVTWLSLEGRGQFE
                     QADYALYTAGTLCVYLLWLMLTTPLPRRRR"
     gene            42744..43238
                     /locus_tag="PKL17_01960"
     CDS             42744..43238
                     /locus_tag="PKL17_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43519.1"
                     /translation="MKFAHLALCSLLLGPWAHADIASPQPKIAVPTPHKAQPAKPQPK
                     QPQPQVPATTDSPRDPVSVARKTPAIAERIAKLKGASAGLYNDQAGAQLDEKVTVIEL
                     DGQCGFAGCSSTSLVVFTYRTRGANTATSSVMAMVSCPPIMQKGCTAAVAEVRPQGQL
                     EQTR"
     gene            43245..44417
                     /locus_tag="PKL17_01965"
     CDS             43245..44417
                     /locus_tag="PKL17_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNK43520.1"
                     /translation="MPPSRTQVVEPLSPLPSAFSASLFVAGVPGPWTHVVVRTLEQAG
                     WDGVVYLPTVNLSSVADSGKRSELVRWRDEALRHSDGVLIWLGDGGKDDPWLFELWGA
                     WQRSSRVVLGVPRDQPVPDSASRLHIPIARSPVDAVEQALQMLRPGQQRRGGERTVPL
                     LLWRSPGFAAWYQAQRKAGHRLEDAQLEWSYRSRAAGRPPLLWALRPRVFVVGERRHK
                     SGEVVIGRTDVSASVLFSPAKSGNIFDAKVVLVREYRSAVRNRLGFGLMLPGGSAAMA
                     SERNTDPRQTAQREVAEETGLQIDPAQFEPVEAGDRQVAASLASYHCHLFRVTLRSDQ
                     LQALEATAASGKSNGANIGERCYVTVKTVRELLDSSDLDWSQLGMVLHALGVPPPT"
     gene            44470..45252
                     /locus_tag="PKL17_01970"
     CDS             44470..45252
                     /locus_tag="PKL17_01970"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNK43521.1"
                     /translation="MSTPVSTPNVQLSGKVAIVTGASRGIGEAIAKSFAHHGAKVVLA
                     SRKLDGLNRVADAIRQAGGDAIAIACHTGQPDQVEKLVSDTVAHFGKVDILVNNAATN
                     PYFGPMLSVDWSAWDKTFDVNVKGYFAATRAVAQHLIGRNAPGSIVNVASVVGFIGSP
                     MQGVYAMTKAAVISMTKTLAMELGTAGVRVNAIAPGLVDTKFASALTQNEEILNMVLQ
                     RTALKRVGQPDDIAGAALYLASDASQYLTGHTMVVDGGWTVA"
     gene            45328..45981
                     /locus_tag="PKL17_01975"
     CDS             45328..45981
                     /locus_tag="PKL17_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43522.1"
                     /translation="MPLARFGSLRPVCAVVALSLAVHGCAAEAVSLSSESDPAGTSYQ
                     KSLDRWSRVGRVISKTEFDTQIVVVATLRSKSFQRAYVDKYLQTYAIVDRWERARVEK
                     EQAEQTQGGLSMFIRSTTHTASWNDLRPQASRWRLSLLDDQGREVQADRVEPLTSRQT
                     VEQTLLTGQSDAFTKLWTVHFPEKASDGQPFPLPGARQISLRVAGPLGQTELPWLLQ"
     gene            46031..48115
                     /locus_tag="PKL17_01980"
     CDS             46031..48115
                     /locus_tag="PKL17_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK43523.1"
                     /translation="MIALEVLSGSLRGQRFLIREASFTIGRAPHNNIVLPDVHLSSEH
                     AQIFREDDKFIFKDLRSTNGSRIQRRDGAVHYVDATTGFESVVCEGDRLLLGDPLDPV
                     ILGCVLSDNQLAPMTVPPGGSSPTVPALQLGRAAPAPPSGPAKPSEGESTEPEGRVLA
                     RRPLTEAEAVAGKVERDPVLATGLLALSKRMGRRGLDLQAVFEGIAESVFELVPLSTH
                     IAIELTDGSEGKMATVFGQTRKSDPKADATKDPKREPVTDGKPALPIRASRAVIRRVL
                     SERAAVLIANAPQDLAGAASIMGARIQSIIGVPLWDGDEIRGVIQCDNRSSAAMFRER
                     DLEVLLVLAGQASLAIENARLHQRLRLAEEQLRAENRYLKSREEKRRTVEPIGQSPAF
                     REVLKQVQKVTDTRATVCIEGETGTGKELVASLIHGQSSRRDKLFVAQNCAAVPDTLL
                     ESELFGHKKGAFTGADADKKGLFEVADGGTLFLDEIGEMSLGLQAKLLRVLQESEVRP
                     VGATQPRKVDVRILCATNRSLEKEVAEGRFRQDLYYRLKVYPIRLPALRERREDIGLL
                     AEHFLQKYSSEMKKPVAGFTSEALAQMAAYNWPGNVRELENEVQRLVIQVEPEAFITP
                     DLLAPRMRQAEGLMDRIAPQKGQLKDMMEEVERFLLIQALKDHGGNKTRTAETLGITR
                     EGLHKKLAKFGL"
     gene            48196..49110
                     /locus_tag="PKL17_01985"
     CDS             48196..49110
                     /locus_tag="PKL17_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43524.1"
                     /translation="MATFILSYPDKAFVPHGISDQAASAPSGAAFAQWLRLCDEIHRH
                     QGRILVIDPEAVGPVSPVYAALLGSPFLSPGKLPQPVFLRANHGEPQAEDAVQRAISD
                     AGLQVRAAQHRWQGQVDLIPVGRNRFILTYGSGDHGSSLHAIEEIKELLPLGAQVMAI
                     ELSEGCPRGSAALTCITDPSNKSVVLVSRRALKSHTPESIGPFVGNNVEVSILSNEDA
                     SVFASECINVRGTLILPVGCSTILRGFLIRRGFQLAEVDVSLLVGPQGGGPRLLACEL
                     PGLVLSDEAPSYLLRRERLHILASEYPT"
     gene            49144..49842
                     /locus_tag="PKL17_01990"
     CDS             49144..49842
                     /locus_tag="PKL17_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HNK43525.1"
                     /translation="MRLLGRSFVLGLISLLAVPSAQAIDHQDAPSLAATPSADIADLF
                     AWMSTDGSKVILAMTVNPGATSSSRFASDTMYVFHTSSRASIASTTATLVDIVCGFDD
                     AQKISCAVGDASVSISGDASNPSGLARSDNKLRVFAGLRKDPSFASIANIDQMKAAAK
                     TALATAAAPDAAGCKATTGKTAIMALGSMPASNTFTTSNVLSLVMEVDRSLLNRGGPI
                     FGVWAATHKKAKTN"
     gene            49853..50560
                     /locus_tag="PKL17_01995"
     CDS             49853..50560
                     /locus_tag="PKL17_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43526.1"
                     /translation="MKRSFFPSLFVLFVGCAAEVPAVPPCSTTVCPSGQVCNPSTGAC
                     EVQPSRCASVSCVSPLTCDGTTGFCVFPLGDLIDRAGRPLINLAVTNPFDLLTVNGQL
                     EASATTRERYNKDKSFPWSAWATTLAPTLALYDGLDGTCGHTLALPALGASRFASMAS
                     LLAADAIIVDTSKSTCAGYLTVESKALAAMPSSDCGGRKLDYDVVDATFSLLTGGPAT
                     DGVPMSNSPLSTFPYLP"
     gene            50613..52934
                     /locus_tag="PKL17_02000"
     CDS             50613..52934
                     /locus_tag="PKL17_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNK43527.1"
                     /translation="MNAAQPTAPDADIAIDPICKMKVDRRAPKGGSHDFLGTTYFFCN
                     PRCREKFSADPERYLYATKPQPNKAAEGTLWICPMCPEVRETKPVPCPSCGMALEPDQ
                     IDLSAPPDAKNHELLQMTRRLWASLALSLPVFGLEMGSMLLIGHHVPLWIVVVEALLT
                     TLVLVAGIELWRRGYVSFRSRKLNMFSLITLGVATAYGYSMVRLGQYLLLGRSGHPPA
                     VYFESASVITSLVLLGQVLELRARHKTGEALRALLTLIPPLARRVFPSGDERDVPVGE
                     LFPGHRVRILPGQRVAADGLILEGQSTVDESLLTGESLPVAKSPGDRVVGGAINGTGA
                     LLIRLTEVGADTLVGQIVQAVQKAQRSRAPIERMVDRASALFVPTVFVVALLSFFAWL
                     WLGGIGKLPLAVVSAVSVLIVACPCALGLATPMSILVGSGRGAQEGVLFRDAEALERL
                     ALVTKVVLDKTGTLTEGKPTLAALLPRDDVSEDRLLSVAAQLEQGSEHPLSIAIVSAA
                     RDRNLPMPTAKQVVALPGRGLRGDSEEGLIVVGTLHLLDEEGIAVPPDAVGQVSLLQQ
                     EGQTVVQVALAGKWLGAIAVHDRQREGVDETVAKLRKLGIGLVLATGDNEKTAQAIAH
                     KLGIGELHASLTPHDKAALVQKLHADGHCVAMVGDGINDAEALASADVGVAMGTGTDV
                     AIKSAGVTLLHGELRSLLKAIALSKLIRRNIRQNLGFAFGYNLLAVPIAAGVLYPLIG
                     ALLSPMIASAAMSLSSVSVISNALRIRNATLDT"
     gene            52967..53296
                     /locus_tag="PKL17_02005"
     CDS             52967..53296
                     /locus_tag="PKL17_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43528.1"
                     /translation="MNPARFLPLAVLFVVTSCYPESDLQPESLRTPADCGARERIDAQ
                     VKNQALLDFASIGKQCEPFAVQTFVQNKQETVWKACCYDKAFNYAYYPAKCVVRKVSG
                     PVDCTPN"
     gene            53449..>54609
                     /locus_tag="PKL17_02010"
     CDS             53449..>54609
                     /locus_tag="PKL17_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43529.1"
                     /translation="MQKKETKLGRCRSSRRQPWLFSVLGSAALLGACDGAPTEVEQAA
                     VEVTTEGESLAPDVTPRCITVKQGDPTSCKDEKTWRSYGEMTCKSKSLSLKSVTFATS
                     CGMASWREATYTCCDSVTPPPPPPPPPPPPGMCITQELSTAAGACIDDGTWKLKAEEF
                     CKSKGLSVKNLGFGTACRSGHINVKVECCGPTPPPPPPPGMCFSEALKDPMGACVDDG
                     SWKSRAEAVCKAKGASVKSLAFGTACRTGHFDVKVECCGGITPPPPPPPPPPPPVRCA
                     WLPVPGTGVCRLPDVWKREATTLCSTKKQTLKDLKLDKACAMGGFTDAQAECCDATMP
                     GPTPPPSTCVGAVLGDGMTCSTEANLKAEADKTCSARGMKLDGFAAYDPCGAV"
BASE COUNT        10696 a        17169 c        16263 g        10481 t
ORIGIN      
        1 cgctggttcc atcttcccga accacaaaca ggccaatgag ggcatccccc agaccttttt
       61 tcttttcggc caacgtatcc tccgaaaaca ggctacttcc ctgcgtgctt tgtcgtcaag
      121 cgagcggccc gaccgccgct gccgcaggtt tcaaacagac agcccccgcg gttcattccc
      181 gatcgccacc acatgtgttg tactgtgcgc atccttcgtc ggtgtctagt gctgcttctg
      241 cgtcgtcttg ttttggctgg gatctttttg gctgcgttcc aggcgtcggc caagccgcgg
      301 cctgtgcaaa gagacacgcc gcggcgtttt tttgtccgct tgcagtttct gtccggtctt
      361 gcgccatcgc tggtttctga cggaaatggc caaagccggc tgaccgcgtt tgtgccgctg
      421 gatgtcgaac tggcgacgcg gctcgatggt gtgctgtcgc tggcagccgc ggttcagacg
      481 gaacttgcgc ccttttcact gccaaactgc cccgatggtc cgccgctgcg gccccatgcc
      541 gcgtcaagcc ttgtcggagc gcgaattgat ctgaacaaca accgcgatgg atcgtggtgg
      601 tcgccgtggg tggccttgcg catgggaatc ggtggacaga tcgtttccga cggaagctgt
      661 gcgtcgtcgg tgcggctttt tccggcgttt tcaccgcgac ttggcgccga tctgtggatg
      721 ggaaagtctg cggcgagctt ttccgtcgga tatgactact tgccgaccgg ctcgctggtg
      781 acgctgcaga tcggactttc gctgcgggtc gattaggcgg gattacttct gcgctggatt
      841 ctgccgaggc ggtggcggtg gaatcgtgac gtcgacggtc tgcaccggac ccaggtgttt
      901 aagctgcgtc agctgatcgc cgatctcgtc gggattgccg accaccagca ccgcaaaacc
      961 gccgggattc aggtactttt gcgccactcg cttgacgtcc tcgagggtga ctttttgaat
     1021 ctgatcggcg tacttctcgg tgtaatcggc aggataaccg gcaaattcca gacgcatcct
     1081 gtccgacagg atcttggcct tgctgtcgaa cttgaagaca aacgagttga ggatcgcgtc
     1141 tttcgctttc tgtagctcgt cgggcgtcgg cggattcatc agcatgcctt gcacctgctc
     1201 gaacagcgcc gtaatcgact tgcccgtcgt ctggctcttg gtcgacatcg cgactttgta
     1261 aagcgacgga aattcccagc ccagaccgac gccaccaccg acgttgtagg ccagtccctg
     1321 cttggtgcga atgttcgaaa acagccgcgc ccaaaaacca ccgccgagga tttcgttcat
     1381 cacctcgagc gcgtagtgat ccgggttttt gcgcgtggtg ccaagctgaa ccaagtgaat
     1441 cgagctttgg ttgacctcgc gcttgggcac aaagtaaagc cctggccgcg tcgggacaaa
     1501 gccaagctcc ggcagcgtca ctttctggct ctgctgaagc gtcgccagct tctgcttgag
     1561 cagcgccaac agctcgtctg gatcgaaatc gcccgagatg ccgacgatca ggttgcctga
     1621 gtggacaaac cgacggtgcc acaaaagcag gtcatcgcgc gtgattccag cgatggtggc
     1681 gtattcttcc tgtcgagcca gcggatggtc tttgccgtaa gccagcttgg tggactcgcg
     1741 accggcaatc tcgctcgatt cgtcgttgcg ccgactgatc gcggtgtaca cctgccgctt
     1801 ggcgatctcg attttgtcct gacgaaactc gggttcacgc aaaagctcga cggcaagccc
     1861 gagcacatcg gcaaagtctt ttttcaggca gtgcgtggtc agcgtcgttc gcgtggcttc
     1921 gctgcctgtc tcgatctgcg ccgcacgcag cgccaaaaaa tcgtcgattt ggtcgccggt
     1981 gcgcgacttg gtcccgcccg atcgccatga ctcggcaaag atgtcggaaa gtccggtctt
     2041 gtcgaacggc tcgtccttga caccgccgcg aatcatcacg ttcatcgtca tgagcggcag
     2101 ctctttgtcc tgctgaagca agagcacaat cccgttgtcg agcaccacac gcacaggctg
     2161 ctgcggtttc atcgcgtgca gcggcggcgg cttgatctga cgccagtcgc ttttgaccac
     2221 cacgggcgcg gcccccaccg gcggagcgag caaaaggctg cccatcacca gcgcggaaag
     2281 cagggaatac gagttcatgg ttacttgcct ccctgcgccg tgctctcgat ccagacggcg
     2341 gttcggttgc tttcgatgaa gacggcgcgg gcgatgcgct ggatgtcttc tttggtgact
     2401 ttgtcgatgc ggtcaacgcg acggaagact tcacgccagt cgccgtagcg ctgctgggcg
     2461 ctcgccatcg caatcgccag cgcttggttg ctgccgaggc tggtcaaaag tcgcattttc
     2521 gcgcgagccc gcaccatctg aagttcttcg tcggtgacca gcgaattttt cagtcgttca
     2581 agctcttcgt gaatggcggt gcgaacctgc ggcggcgtca cccccggcgt cggcacggca
     2641 tagaacgcaa acaggtgcgg atacttccga cccggcatgc ctgaaaaacc tcccgaggcc
     2701 gccgcgattt ttttgtcccg caccagcgag cgatacagcc gtgccgtccg tcccgtcgac
     2761 attaggtcag cgatcgcttc gtacacggcg tcgtctttgt caaacacgtc gggacggtga
     2821 tagccctcgg cgtaaaatgg ctgcgccgtc tcacgcagga tcacttggcg ttcagcgaac
     2881 tgcttcggct cgacggtgga cagcggcgcc ggcttcggac cttttggcaa acggccaaag
     2941 tacgactcga gcattggcat cgcctcgctc gctttgacat caccgacgat cgcaatcgcc
     3001 atgttcgccg gcgtgtaata ggtttcccaa aacttcagcg catcggtggc cgaaaacgtc
     3061 gccaaatctg ccggccagcc aaccaccgga cgcccatagg gatgcgccgt aaacgccgcc
     3121 gccaagagct gctctaacat ccgcccaatc ggacggctat cggtgcgcat ccgtcgctct
     3181 tccgtcacaa cgtcgcgctc ggtgtaaaac tctcgcatca ccgggcgctt gctgcgctcc
     3241 gactccaaat acgcccacag ctccagccgg ttcgacggca gcgagtaaaa gtagcccgtc
     3301 tcatccgagt tcgtaaacgc attgagcccc acaccgccat tgcgatcgac cagctcaccg
     3361 aactcgttgg cgacgacttg cttgcttgct tcggcgatcg cgtcgcgcca agctttttca
     3421 agcgcttcga cctttttcgg atcgcttccc gacggcttgc gtcgctcggc gtcgaaggcc
     3481 aaatacgctt tttcgacctt gtcgatcagc accttttcag cgacgaagtt cttggtgccg
     3541 attttgtcgg tgcctttgaa cgccatgtgc tcaaacatgt gcgcgagccc cgtctgaccc
     3601 atcgcttcct gcgcgccgcc cacgtcgacg tgcgtgaagt atgaaaagac cggcgcttcg
     3661 ggacgctcga cgacaatcac cgtcaaaccg ttgccgagct gcttgacctg ggtgcgcttt
     3721 tcaaagctgg cgaggtcttg tgcgtgcgcg accccgcttc ccagccccgt cggcaccatc
     3781 cccgacgata gcccgatggc gagcgcaaaa ccaaggagat gacgtctcat tccgactcct
     3841 gtttccttcg ctatgtccgg cacatcgaac ctgtgcgacc ggaccagaac attccagatg
     3901 tagggcagtt tacgtcgcaa tcgggccaag ctaaacaaaa tcccgcgcga cagcggtcag
     3961 atccgtgcgc taggcttcgg gtactttgcg tcccaatgtt ccatttgggc acataaacgg
     4021 agacgtccac ccaaccccaa aggaggcacg catgaagact cgtttgacac ttttctcttt
     4081 gttcctcgtc gcagcttgtg gaaatgcgga tcccagcccc aaccaaaacc cggttattgg
     4141 tgaaagcgcc gtcacgtccc ggcagccgtc ggaaattggg caggccctgc agatcgccgc
     4201 aacgcagttt gcgattccgc aatcggtgct gcaagcgacc gcttacgccc aaacgcgact
     4261 gttctttgtg cccggccagg ccggtgaagc gtcgggcgat gagccgcagt acggcatctt
     4321 cgccctgcgc agcgccgcga tccgaaaagg cgcccagctg gctggcataa gcgaccacga
     4381 cgcacaaacc accgtgatcg gaaacgttca ggccgcggca gcacttttgc gcagctatgc
     4441 cgacgaagcg ggcatcgatc gcaccgacct tgcggcctgg gccggaccca ttgctcggct
     4501 gtcggcactt ccgaccgaac aagcacaggc cgatttcatc cacaacgtcg tctacaagac
     4561 cctgcgtgag ggtctgccgc cggaaatcgc gctcggtcac gggctttccg acgcaccaca
     4621 cccggacctt gtgccgcagt ttgccgcgct cgtcgacgat tcgcttccct acgccggggt
     4681 tcgttattcg ggatcggtct ggaaaccagc cccttcatcg aacttcaccg tcggtcgcag
     4741 cgccgccatc gatctgctcg tgattcacac ttgctcgggc ggttattcgg gctgcgtcgg
     4801 ctggctgcag acgccctatc cgacgaaccc gtacaagact tcggcccact atgtcgtcaa
     4861 ggaagacggc accgagatcg ccgctctcgt cgacgaaagc aacaccgcgc atcacgtcgg
     4921 tgcgtcgtgg gaaggcagac caaccaaccc gcgttccgtc ggtatcgaac atggcggttt
     4981 ttcgtatcag ggcacgaaca agtggaccga gggccaagtc tctgcgtcgg ccaagctgtc
     5041 ttgcgacgtg gtcaagcgca accgcatccc gcgcgaccgc aaccacatca tcggtcacta
     5101 tcagcccgat ccggtcaacc gcgccgacga ccccggtcgt gacttcccgt gggccgatta
     5161 catgaatcgc atcagccgct gcgtcggcgg atcgacggcg atcgttgtcg atagcaacca
     5221 agcgaacaac gacgccagcg gcaagatcga caccccgagc agcagctgga cttcgtcgac
     5281 gaatgtcgct gggtattatg gcagtggcta ttacgtcgct ccgacggcgg cggtgtctga
     5341 cgcggtgtac tttagtttct atctgaccga ggctgccaat cgcgaggtct tcgcctggtg
     5401 gactgcggct tcggatcgca gcaccgagac gccgtttgtc atcttcgatg cggcgggaaa
     5461 caagctcgcc accgtcaaga aaaaccagca agacaacggc agtcagtggg tgtctctcgg
     5521 acgctacaac ttcaccgtcg gatggaacca agtcgccatc tcgcgctggg cgaccgccgg
     5581 atcagttgtc gtcgctgatg ccatcaaagt cgagtaacgg cgatgctggc ctggctcgcc
     5641 gctgcggcgg cactttcgtt tatctcggaa cgtgatggca accgcgaggt ttatgtcatc
     5701 gcagccgacg gaagcggaga acgccgaatc acccgcagcg gcgatgccga ctacaatggt
     5761 ccgatcactc ccgacggaaa ataccttctg gttacgtcgg tgagtgagct tcagcatgaa
     5821 aaacagcagc gctttttcct gtatccgacg gcaggcggcg tgccaaagcc gcttgggccg
     5881 attcgatcgc tgctgcgcca tcccacgttt tcccgcgacg gacgcttttt attttttgaa
     5941 ggttcgtcga cggatctgcg tgaaattttc cgtctgcaaa tcgatgggca gaaactgatc
     6001 ccgatcacca aaaatcgcga aggcaacttt cagccgtgcc tatccccgac gggagatcgg
     6061 ttagcttttg tgtcaagccg cgatcaagtg gccgagctgt atgcgtcgtc tccgacggga
     6121 ggtccggcgc gacgactgac ccagacggaa cgggacgaat ggcaaccgca gtttttcccc
     6181 gacgggaaat ggctgctgtt cggcagtgac cgcgatgggg ctgaccggct ctacaggcag
     6241 cctgtcgacg gtggcacggc aaaacgactt tcgcccgagg gtcttgatcc gtttgttgtc
     6301 gaggaatcag ccagcatttc gcccgatgga agcgcggtcg tctacgtgca ccgtcagcga
     6361 gatcgcaaag acaagctctg tctagtggac gtggcgacgg gaaaacgcac gacgctgttt
     6421 gaaagccagg ccgaccccgc gaccgagcct gtgttttccc cagatggaac cctggtcgcg
     6481 ttcacgctcg ggctggggtt gcacagtcag attttcgtcg tcgcccgtga tggcacaaaa
     6541 ccacgtcggc tgacgcaagc caaaggtccg aactggcatc cgctgtggct gtcccgacct
     6601 agcgctcgat aggaatttcc accttatcaa cctgcggttt gctcggggct ggcgcagtcg
     6661 acggtggtgc agcgggcgcg ctgtcgacgg gtgcggcggg cgcaggggca gcggcgcgat
     6721 tttccggctt ggcgacgacg attggttcct gaactttcgc gctgtttttc ttgcttgctg
     6781 ccggtccgcg acgcagcgat tgtacttttt caggtgtcga caccacgttc acgtcggaac
     6841 ggaccgaagg agccaagtca ggtagcgacg acaaatcatc ggctcggcgc agatcgataa
     6901 acgacgcggc gcgggacgaa acggccaaat cgaggcgtgg tgtttcgaca agtggcccgg
     6961 ttccgaaatc gggcgggcgg ttcgtcgtcg gtgacacgcc ccatcgcaga gccaaaccag
     7021 cccccgtcgc caagaccgcc accgacagcc acacccagcg attccgtgtc gacggagcat
     7081 gcgcgacgtt ggcgttagga tcgacggaag acgatggcct ggcggggaca gcggcctcgg
     7141 caactggcgg cgcggaaaca gcgacggatt gcagcgctcg caagagctcg ccaccatcgg
     7201 cataccgcag ctttggatcg cgggcgaggc agcgatcgat cacctcggcg agcgcatcgg
     7261 acagtggatg atcgggcaaa aggccgcgca ggggtggcgc ctctcgcagc cgtactgcga
     7321 tggaaagtcg cgccaaatcg ggttcgacaa acggcgctcg acccgacaaa agttcaaaca
     7381 agagcgcccc gagcgaatag acatcacttc gcgccgtggc ggcctcgccg gcaaaaatct
     7441 ctggcgccat atatcgcggt gttccgacga gcgctccggt cttggtcaga gacaccggcg
     7501 tcggttcacg cagcgccgaa agcggctcgt caaaaggggt cggctgttcc gcgggtgcag
     7561 cttcctcggc agccgaaagg gtgacatcgg aaggacgccc gctcgtcgac gtggtacgcg
     7621 atttggccag cgcttccggg gtcaacgtcg cttcgtcacc gcttatgaca tccgcagcgg
     7681 gaatggctgg ctggctgctt tcgccgtcgt cgctgcgcag cggatctgac accagcttgg
     7741 ccagaccaaa atcgagcagc ttggcatcgc cactttcggc gaggatcgcg ttggccggct
     7801 tgacgtcgcg atgcagcacg ccgctggcat gcgctgcttt gagcccggca cacagtccca
     7861 gaccgatttg caggcctttt tcgacggaaa ctggcttggc aagctccgcc aggctgtggc
     7921 cgcgaacaaa ctcagatacc agatagggac gaccgcccag ctcaccgacg cgatagacac
     7981 tgacgacgtt gggatgggtc agccgcgcaa tcgctcgcgc ttcgaccaaa aaacgctcac
     8041 gcaccgtcga gttgggatct gccgcgatga acttgatggc gacgggccga tcgagcagcg
     8101 tgtcgtgcgc cagatacacc tgacccatgg cgccacgccc aagcagccgg acgatccggt
     8161 attcagcgac ttccgacgga ggtgtaaaca caggccgcat tgccagcagt caggttcgat
     8221 attcgtatgt cctagccgat atcatgcgcc agacgtcgag ttgattgaca gagcagccaa
     8281 acacgaatct aaatgggcgc atgcttcgcg atactctccg ccgcgtcgac cccgagattt
     8341 tttctctgct tgaacaagaa gaccagcgcc aacgggacac cattcgcctg atcccgagtg
     8401 aaaactacac ctcggcggcg gtcctcgaag cgctaagctc ggcgctttcg aacaagtaca
     8461 gcgagggcta tcccggcaag cgctactacg aaggccagca gttcatcgac caagtggagc
     8521 agctttgcat tggccgcgcg aagacgctgt tcggcgccga acatgccaac gtgcagccgt
     8581 attcgggctc gccggcgaac ttggcggtgt acctggcgtt ttgcaaaccc ggcgacacca
     8641 tcatggggct tggtcttccg gcgggcggac acctgacgca cggctggaac gtgagcattt
     8701 cgggcaagta ctttgtgtcg gtgccatacg gtgtgcgcaa agacaccgga cgggtcgacc
     8761 tcaacgaggt ggcggagctt gcgcgcaagc acaagcccaa ggtgctgttc tgcggtggga
     8821 cggcagtgcc ccggacgatc gattttcccg gctttgccca aattgcccag gaagtcggtg
     8881 cagttctcgt cgctgacatt gcacacatca gcggtctggt ggccggcggc gcgcacccga
     8941 cgcccgtggg tcacgctgaa gtcgtcacaa cgacgacgca caagacgctg cgcggtccgc
     9001 ggggcggcat gatcctctgc aacgccaaac acgcgcaggc gcttgatcga gcggtcttcc
     9061 ctgggctgca aggcggtcca cacaacgcga cggtggcggc tctggcggtg gcgctcaaag
     9121 aagcgcagag cgaagacttc cgacgctacg cccaccaaat cgtcaaaaac gccaaagcgc
     9181 tggcgacggc gctgcttgcg cgcggttatg agctggtttc cggcggcacc gacaaccacc
     9241 tgatcctcat cgacctgacg aacaagggca tcgccggcaa gcgggcagcg caagcgctcg
     9301 atcgcgccgg tctggaaacc aactacaaca gcgtcccgtt cgatccgcgc aagccgtttg
     9361 acccgtcggg cattcgcatc ggtacgccat ctgtcacgtc gcgcggcatg ctcgaaagcg
     9421 acatggaaca ggtcgccgcg tggttcgatc gcgcgatcga agccgaaaag cagtccgacg
     9481 cccaagccat cacccgcatc gccggcgaag ttcgtgagtt tttgctccgc ttccccgccc
     9541 ccggactcta gttttcgaca gaggcggcac gatgacacgc ggcacagcgc gggcagccgt
     9601 cgggctgctc ctttttttct gcggacaggc agcgcatgcc gaacccgcgc aagctccgac
     9661 ggcagctcaa gctccgacgg cagctcaagc tccgacggca gctcaagctc ccgcagcagc
     9721 agtccagacc gaagttccac acaagctgac cccggtgctc agcgacgatg agctggggaa
     9781 actgcaagcg ctgatgctgt ccccgcaaag cgcagtgcga ctgcaagcgg cgcagaggat
     9841 cgcgactgcg gacattggcg acggagacgg agaagcgcgc tacgcgacgt ggctgcgcaa
     9901 agagctcgtc ggacagaccc aggtgatgca gaagctgatc cacgccatct ggggtcagta
     9961 tccaaatccc gattatccgc gcggaccggg caaagatccg ccgatgtgga tgatccgtcc
    10021 cgagccgccc attccgccgg gaaccgcgaa aaaacagaag cccaaaccgc acgatcccga
    10081 agcggtcgac tggcttttgg cgctttcgca gctagacccc gcccaggagc cgattctcca
    10141 gggctttccc gtcgaagaag cgaacaagac acgggccgag ctgctgctca aagtggccct
    10201 tttgcgcgcg ctgtcacaag cgggtcagcg cgggtccaga gaggccgtgc atccggtgtt
    10261 cgaacaggca ttcgtgcgcg acggggtgct gcgcgacgag tgtggccgga tgattcgcgc
    10321 aatgggcagc ccggcgattc cggggcttgt gcgcatttac aacaaccgca gccgcgccaa
    10381 ctacaaaatg cgtcgctacg cttcgtatca gctcgatcga atggatcggc tgcgcccgag
    10441 caaagcgatt gtgtccgcgt cggatgactt ggtgcgcgcc gacatcatcc atgcctacgg
    10501 cgaagtactg gcgatcgatg cggtcgaagc gattttggaa caagtttcgg cgcggtctcg
    10561 gcgggtgcgt cgcgaggcgc gctgggcctg gctgcgctat gtcgacggtc cgccgccgcc
    10621 gccgccgccg aaacgcaagc gcaagctgcc cggcggaaaa gaagaagccg aggaaaaaga
    10681 agactaccta agctaccgcg agctggcgac gctggcgctg cgacggaccc acaaagcggt
    10741 gtttggcgag gaaccgtcgg acaagctctc ggcgaagcag cttaccgacg aactgtttgc
    10801 ctattacgac aagcaggaag aagcgctgtt tgcgaagctg tttgcgtcag cggaagaaca
    10861 cctgtcgacg gggcagccgc agcttgcggt gtcggaattt ggctggattt tggccaatca
    10921 gcccgatcat ccgcgtcgcg tcgagatggc attggcgttc cgcaagtacg gcgagcaagt
    10981 caaagccgaa gcagaacaga aaaatgacga cggtctgcgc agtcaagcgg tcggttggct
    11041 gcggcacgcg gtgctcctgt cgtcgaacca gcccgacatt gcgaagctgc gggcgcagat
    11101 tcatctgctc gatggcaagc tcgcgctaaa acaaggcggc gacggtcgca gcgattttgc
    11161 cgcggcgctt cacgaagatc cgagcctgtc agaagcgcgc acgtatcttc accgcgtcgt
    11221 gacccagccg cagcgacgca tcgactggct gcgctggctg tgtctttcgc tgctcgcaag
    11281 cgggctgttg ctgctcggac tgtggcgccg acaaaatgcc tcgctgaagt agtttttgac
    11341 cgcccgtcgc gtcttcctta cgattcccag gttcccgcga gggaacacaa aaccccaaac
    11401 ggaggacgga cgatgaacaa ggttgtctct ttcgcgatgt tggctttgct ggccttggca
    11461 gcggcgtgtg gcccgaaggc gcaagctggc ggaggaacga cggtggcgcc acctgtcggc
    11521 ggtgccccag cgagcgggac gagcggtgcg gctccgtcgg gcagcggcgg gacggacgca
    11581 gcgccgccga tgaatgcaaa ccccggatcg gccccggctg cgccctaacg tctgataagt
    11641 cccgccgcta cgggctcgac tcgtcggcaa gaccgccgag cgacatcact tccgccccca
    11701 ccgtcatgag cagtcgcgga tcagcaaaca gattcagcga tggcgcaagc agcttggcat
    11761 agcgatcgaa atacaaaagc tgccgcgtga tcagcacaaa ctcactcggc atgtgggcgc
    11821 cgtgccgctc agcaactttg agcagattgg taatcacttc gctgtagtcg atcccgccca
    11881 gcgttccccg caaaaagggc tgatacgcga ccttcagatc ggcagcaaat gtggtccatt
    11941 cctcgtcgct gaccttgcgc gccaccgcgc ccatcgccgc catcgttttc gcaagctgca
    12001 taaaatcctg cgtcgcaaaa ccgagcagat agctcgcgat ctgtttgcgc cgcgtccggt
    12061 caagccgccc gatgattccg aaatcgagaa agccaagccg cccgtcgcgc agcgccatca
    12121 aattgcccgc atgcacgtcg ccatggaaaa aaccatagcg gagcatgcag gaaaaccaag
    12181 cgtgcatgcc gtcgatgagc cgcgcttcgg tctgttcacg ggtgatgcca attcgcaaaa
    12241 gtccgtcgag gtcgtcgaca cgcacgccgt gcagccgttc catcgtcagc acatcccgac
    12301 ggcacagcgc acgcaagggt cgcggtgcca ccacgtcgga tcgtcccaga tcacgcatga
    12361 tctggttgaa ttcttccatt cgtgacgctt cgcccagaaa gtccagttcc tcggcgatgt
    12421 tgcgctgaaa ctgcgccacg acagcctgcg gattggccgc ccgcaccccc ggtagcatcg
    12481 ccagcacatc ggcacaaaac gacagcatcg acacgtccgc cgcgacaagc tccggcaggt
    12541 gttgacgctg cactttgacc accagatcgt gcgtttcgcc gttttcaacc agggtcactg
    12601 cgtacacctg ggcaatcgag gctgatgcga gcggttttgt atcaatcgtc gtaaagacct
    12661 gctcgggcgg cttgccaagc gcaagcgtga gcgtctgccg caccagttcc caaggttccg
    12721 ggggcacacg gtcgaggcac ttctggaact ctttgacgta gcgttcggga aacagaccat
    12781 tcgagctggc aatcagctgg ccaaacttga tgtaagtcgg tcccagccgg acaaagagct
    12841 cgcgcagctc gacggggcct gccagctgcg gttcgccccg aaagcgccgc aaaagatagc
    12901 gacgaaaata caccaacagc tggaccaacg tgaccacagc ccgtcgaatc ggcgggctgt
    12961 acataaaagc tgccccaagc acgaccaaaa acgccgacac cgcaacccag ggtgccgccc
    13021 ctcccgatag cgcgccgctg cgcaggcaat acggaatggc ggtgtacgaa actgcgagca
    13081 tgactgacat cctagcgcga atttcacgac acgctactca gccagccagt cagcgaccag
    13141 ccggcctgag agccgcggag tgtgtttttg gggaaaacga ccgcagagag gcagagcggt
    13201 gaagagaagc cggtctagct ggcttttttg gcctttttgg ccggtttggc ggcttttttg
    13261 tcggctttgc ccttcttgtc ggccttgccc ttgccatcgc tttggtcggg cgagtcgggc
    13321 acctggactt cggcagcgct cttggtcttg acgttctgcg gctgcgccgg atccaagatc
    13381 acgaactcga cgcggcggtt ccgttcgcga cctttcttgt ccttgttgtc ggcaatcggc
    13441 ttgtccggac cgtagccttt ggccgtcagc ttgtcggcag caatgccgtg atcaaccagg
    13501 tatttcacga cgctgtttgc ccgccgtccc gatagatccc ggttgtagtc cgctttgccg
    13561 cgatcgtcgg tgtgtccgcc gacttcaatc ttcttgatga acggcgaagc cttcagcgcg
    13621 tcgaccacat ggtttaggac cgggaagctc ttcttgagga tcgtgtcctt gttcgtcgca
    13681 aagaacaccg gctccatgat cgcaatctgg ccgttttcga tgcgaaccag gccggggcag
    13741 ccgttctttt tcggatcggg gctcggtgca ccgggctgat cggggcaagc gtcttccgga
    13801 tcgaccaccg tgtcgcggtc gcggtccggc agcgggcagc cgagcttcgc cggatcgggc
    13861 ttgtggcctg ccggcacgtc gggacacagg tctttcggat cgggcacgcc gtcgttgtct
    13921 ttgtctccgg ctggacaacc caggcggctc ggatcgggcg tgtcaccctt gggcacgtcg
    13981 gggcacagat cctgcggatc gagcacgccg tctttgtctc gatcgcccgc cgggcagccc
    14041 aagcggttcg gatcaggcgt cgcgcccttc ggatcatctg ggcagatgtc atcgctgtcg
    14101 agcacaccgt ctttgtcgcg atcgcccacc gggcagccga gccgcttcgg atcgggcgtt
    14161 ttgcctttgt gaacgtcggg gcagatatcg atcttgtcga ccaccgagtc tcggtcgcgg
    14221 tccggacagc catgcgtcga cggatcatcg gtgcgaatgc cagcgttgtc cgggcaagcg
    14281 tcttccttgt cggaaatgcc gtccttgtcc cgatcgtcgt cgccgtcttt ccacggtgca
    14341 tacgccaggc gcaacagcac gcgaccgtcc ggcgttcccg gttctcgcaa aagacccaga
    14401 ccgccaccga cgccaagctg caggatcttg gcaatgttgt agtggaagcc gagcagcact
    14461 tcgaggctgg tgaagtcgga accaaacggt ttgacgctgc ccgtgccgag cactaccgtc
    14521 gaaaaaatcg cttccggacc aatcgcaaac cggcgctggg tatcggcata cgcaatcgcg
    14581 gccccgattt gaagttcgga cccgaccgtg ctgcccacgt cgctgaccgt cgcgttgcca
    14641 atcttggccg gggtccggta cataaagccg gcgccaaacg accacatgac gtggtgcgac
    14701 aggccaccga gtgcaatttt gggcatgatg cgaatgcccg catcgctgct gccgtttgat
    14761 acggcggcgc tgccgtcggc aaatgcccgc agcggaatcc aagcatttgc accgatgctc
    14821 atcgaaacgg cactgcgata cggctgccca aacaggcgca cccacaggcc gacgcgggga
    14881 tcggaaatca ccacgccgga cgccggggcc gcgcccgctg ccggagtgcc acgctcgagc
    14941 aacacaatgg gcatcgtcgc ggtaatcagc acccggtcca gaaacgaccc ggccagatcg
    15001 acgtgcccaa tcagctgatg ctcaatcacc gacagcgtct ggctaaatga acccgtcgag
    15061 tcggtccgcc cgaacaccag cgggttgtgc gcgtagttca gcgtaatgcc cgcggcaaaa
    15121 tatcgcgtcg aggaatacca cggatggtcg acccagaacg accactcgcc agcggctgtc
    15181 ggttcgtagc ggtttacctg aaagccagcc ttgacgtctt gcgcatgcgc gctgggcgat
    15241 gaccacagac tagcgagaga caacgcgcca attgccgcga ctcgccgaac gaaggaacca
    15301 atattcagca caagagcctc cgaattattt ttgtaaagtt agcacgccgc cgcggagttg
    15361 gcctgccgcg ccatcagcgt cggcgaggct atttgacgac gcgaatcttg ttgcctgccg
    15421 agcgggcgat cacacttcgg tcatacatgc tttcgccttg cagcggcggg acggcgaacg
    15481 tgccgacgtt ggtggcgcgg atgccgtaga cgaactcttg caggtcattg ccgacgctgc
    15541 catagagcac cacgcggtct tcccgaacat cgccatattc tggcgtccac gtcgagccat
    15601 ctttggcaat cggcagcgct gccggcggag ccgaggtctt gcgcgcccca cgctcgctgc
    15661 ggctgctgga atccgacgat ccgtgatcgc cttcttcgcc ctcgccgcct tcggatgcgc
    15721 cttcgtgacg accttcatca tcgccgtcgt cgtgactgcc accgccctct tcatcgctcg
    15781 attcactgct ttcctcgctg gcggctggcg gcgtcaccac cacttcaaaa ccaccgggca
    15841 acaggtcgac gagcgccaga ttccaaagct gcgtctcttt gccgaggctg cgcagcttga
    15901 tgtgcacctc tgcttcgtcg cccagcttga cttctttgat cggtttccca tcgctaccga
    15961 cgatttcacg cagcacttcg agcttttgct tgaccacatc cgtcgacacc cgcaggtcat
    16021 agcccgactg cgtcacttgg tagaacgtcc gcagcggact gctcgacgac accagcagct
    16081 tgcgagcatc ttgtgaaaac accacctgcg gcaacagtcc cgtcggtagc tgaagcggtt
    16141 tctgctgtcc gccgctgttt acttcgatga tcgaaagctg gctcacgtcg gtggtgtctt
    16201 gcgccaggct cgcataggta tcgagcgcca aaatcaagta cgccgacgac agcgtgttgt
    16261 aacgcccctt cgcgatcgga tcgacgatgg tcagcagtgc gtccgccggc agcgacttca
    16321 ccttgtcggg gaaatgccgc gccatgatgt acagcgcctg cgcgtcgcga atcagcgggt
    16381 cgtagtagtt ctgatagtcc gacgcgctgt ctttgacctg accaagctga acgctcgcca
    16441 tgattcgctg ggcatcgcgc gtctgcttga gcagctgcag cgtcgccgca agatagattc
    16501 cggccagatc ctcgccccag tttttgtact ggttttcgag ccgcttgcgc acgctcatcg
    16561 ccgcttgcgt cgtcaccaca cccgatcgcg tcagcagata caccgcgtaa gcccgcaccc
    16621 gttcttcact cagcgatccc gtctcacgcg cgacgatctg attgagaaat tcgaagctct
    16681 ttttgagcag ctcgggcggc accggcagac cgcgctcttt ggcttccagc aggaaatgcg
    16741 tcgcatacac cgacgcgaag tcgttcacct gattcggatt ggcccacagc ccaaagccac
    16801 cgtcttcgtt ttgtcgagag cgcagcgtct gcaaaatacc ctggaagctg acgtccgccg
    16861 cttccgtctt gatgccaaac tcgggccgac cgcgaccaat cagcgccggc atcgcctggc
    16921 tgacctgctg ttcggtgcag ccgtgtggga atttttccag atactgcgca agcccctgcg
    16981 acagcccgag cggcagcggc gcaatgccag cctgcaacac ccgatactcg gcgaagagct
    17041 ctcgcggaac ctgaagctgc gtccgccccc cgcccggcgg cagatgacca acctgcgtcg
    17101 tcgtgtaaag cggcgccgcc ggacgcaccg acagatcaat cgaatacttc ccgcttttgc
    17161 cacccagcga cgcggtaaac gtcaggttgc ccgacccaag cacctgtgtc gctttcagct
    17221 tgtagcgcac gaccgattcg tggttttcgc cgattttgat cgtctgcgac gcatccgcga
    17281 gcacttgcag gtgcttcgag gtctgaagct gcacgttcac tttggcgttt gcgcccgagc
    17341 ccgcgacgtt gttcgacacg ccgaccgaga cttcaaactc atcgccgggc gccaccacca
    17401 tcgggacatt tggacttagt acaaagtcac cgcgtaccag cgcttttttg ctccacacgc
    17461 cgacggcatc tgccgacacc gcaaccgcca tcacccgcag cgtgccgttg aacgaatcgg
    17521 gaacctgata cacgacctct ttgtcgtcgg tatctgcatc aatgattccc gaccaatagg
    17581 ccaccggctt gtcgcgcttg cgcttaaacg gattcagatt tttcatcgcc ccgccgtcgc
    17641 catcaccgcc cggcgccgct ttcagaagcc gcgaaaactc cggcaggatc aggtccaaaa
    17701 tctgcgccgt cttcacttcc agcgcgcgct tttggaaaaa gtacgacagc ggatcgggcg
    17761 tctggtattt ggcaacctgc aagatgcctt cgtcgacggc ccacagcaca atcttggtcg
    17821 gacgatcggt gtgatagcgc agcttaaagg catcgcccgg tttcaccaga tccggcgtcg
    17881 tcaccgagat tttcgccgtc cgtttttccc gcgaaatgct aaacggcgcg acgccatagc
    17941 tcagcggact cataaacacg tccgtcgagt cgatgccgcg gatgaacgcc accgagacat
    18001 agccgttgcc ttccatgtcg tcgggaatcg tgattttttg aatcgacgag ttgtgcttgg
    18061 ttttaaacca cttgtagctg cgaacgcggt cgcgctcgat ggtgataagt cccgcgccgg
    18121 tgtaaggagc tttgatctgg atttccagct cctcgcccgc cgacagatcg ggatttttca
    18181 gcttgagctg tagctcggca ttcttttcga gcgaccgcgt caggttgccc tcgcccgcga
    18241 tcgaaaattc aatccggttt agcacctggt ctttgtcatt tttgaacacc aggaagtagt
    18301 cgccggcctg cgtcgtcggc agcggatagg tcagaccgcc cgccggaatc gacagcttct
    18361 tttcctcgac gagcagctct tttttgaccg attcgtattt gtacgtgccg ttgtcttgac
    18421 ggatcagcac cgacagatag cgccgctcaa acaacatcgt tcgcacgccc gactgcgcca
    18481 ccgacgtgcc ttgcggaccg acggcaatca aatggacgtt gcgcttgctc gctttgctta
    18541 cgtagcccaa atctccgtcg gtcttgtagc cgagcagata ggccatcggc gacaccgtga
    18601 cttgcgtctc cgcagcgacg gaacgaccgc cttccgcttc gaacccttgg gcaatgaaac
    18661 gcagccgata ggttgcactc gaaaagcgcg acagcggcag ctgaaactcc gattcaccgt
    18721 gctcgcttgt ctcaccgtct tcgagatttt ccgtcagcgg ttctttggcc tgcatcggat
    18781 cgaagaactg gtagtcgcgg aacttgctaa agcttggcag cgtcggcgac aacatcagcg
    18841 tcgccttgat ccgacggttt tccgccggcg tcccaaacag attggtaagc tgcacgcgcg
    18901 ccttcagacc gtcgggggac acccaacctt ccagcgcttc ccgactgagc ttggccgaaa
    18961 ttttgagccg atcgggcaaa aattcctgca cccgcaccgc gacggaaccc agcaagctgc
    19021 tggtctgtcc gtctttgaca atgtggacgt ttacattcca cgttcccgtc gctgatgctt
    19081 ccagtgtttg gtggcgaatt tcctcgaaac ctgcatcggt cagcttgaat ttttcgcgcc
    19141 gcaccgtcac accgcgcgca tcgtcgatgg acagctcaag cggaacgcca cccagcttct
    19201 gtttccagtc ttggctcttg acgatcagac cgacgcgaat ttcgtcaccg ggacgataaa
    19261 ttccacgatc ggaaaacagg tacgcttgca gtccttgcgg cttttcactg tcgtgaattc
    19321 cgtcgatggg gaagcgcgaa aaatccaggt agcggtcgtg cctgccatac ggcaaaaacg
    19381 acagatcgcc gcccttgctg acctgatacg cgagcggcgc ttgttcgcgc acgaaatcgg
    19441 acagcttcgg gaacgaaacg tgtccttcgg catcgctggt ttcgctttgg atcgcgacgc
    19501 cgtttttgcc cagcacttcg acggtggcgc ccttgatcgg ctcacccgtc ttgagcgact
    19561 gcacaaacac atcgtgcgtg ccgtcgacgt tgtctttgac cagcacacca aggtccgtca
    19621 gcagaatcag ccgctggtcg ctctgtcccg tcgtctcttt tttgtcgggg tcccagcttt
    19681 cgactttgag gaaaaacaga ccgagccgac tttgcccttt ggcgtcgaaa tacttgccca
    19741 gattcaccgc cgcgtactgc gccttgccat gcgccaccgt cggcatcgtc acttcatcgg
    19801 tgaagcgctc ggtgatgttt tccggcgtga agcgatagtt tgaaaagtcg ggattttgga
    19861 acgacccggt gttctgcgaa atcaagtgct ggagctgatt cggcagcacc cgaccaatct
    19921 cgacgcgaac ggcggcaatg tcgcgaccaa acagcggcac cttgcgctca cccgacatcg
    19981 ccaagagagc gcccgtctgc atcaccttca gctctttggg gaactccggc accgtcagga
    20041 tgtgctccga cggacgaccc aacacgtagc cgcccgccga gcgcatgcct ttgttgattt
    20101 tgatgtacag cacctcgccc acctcggcga agtaacggaa ggagtgggtt tccgaaaact
    20161 cgtgctcgtt gggaatctgc tcgactttga ccggacgggc gagcttgagc acgtcggggc
    20221 caatctcgct caggctaaag ccgtactgct ctttcgagct gcccttgtcg tttggtggcc
    20281 gcttcggcaa caccacaacg ctgagcgcat ttttcatttc cgactcggta acaccggtat
    20341 tggtctgcac caccagcacc ggctccggtt ccatctgcgc gttgttgacc agcgtcaggc
    20401 tcgcttcgct gacccgcaga aaatcgtaaa gtcctgggat cgtcaccgac gtgccaatcg
    20461 gttccttggt cggcttgccg ccacgcgacg acttcacccc tttttgcacc cggaccatca
    20521 tcatcgagtc atggtccggg atctgcaccg ggtcggagtg gacatacgcc gtcgcggcaa
    20581 aacgatcgaa tgtgacctgg aagcgatacg gttttaccga ctgcgcaccg tcttttttgg
    20641 tcaccagctc gacctcgagc cgcttttcaa attccgcctt gtcgaccgga tgggtaaacg
    20701 cgatcgtcgc cacgaccttc ttctgcgtcg gatcttgcgg atcttgatag aactcttgcg
    20761 cggtaaaacg acccgtcagc ggcgcgctct ggaagttcag cgagtattcc gtcagccgca
    20821 cgtcgtcggc caaaagacct ttgcgcgcca gcttgactga aaattcttct ccgacgggcc
    20881 agtcctcttt cggaacaaac gtaagcacgc ggtcggaatc ccagcgccag ctcccttgca
    20941 gcgggggcga aagctcaaca ccgacggtga ctggcttctt cactttgtcg atgggcgccg
    21001 ccgagccacc aaacagaatc cgcagcgagt ccggcttggc attgtcttcg agccgcgtcg
    21061 cctcgggcgc atcgacgcgc accgacattt cgatcggctt cggctgtttt tcgagccagc
    21121 gattgcccaa ataggccgcg ccgccaaatg cacagatcat cagcacgacg agcagcgccg
    21181 ccgagcggtg attttgcgcc cagcgcccgc ccgatgccac gccccggcca atcgcggcca
    21241 gccacggggg aggctgccag ctaacttgcc caaacaggcc gcgaccgatg cgtcgaatca
    21301 cgcccggctg gtcgcccgca tgtgcagcag gaaaaccgcc caacccagcg ttaccatccg
    21361 cagcagcgtt tgtggcattt tccgtcgctg gtttgtctga acttggactt tccgaagcag
    21421 gcttgtcact cggttccgtg ctcacgagga ctcctttgac aagcgacaag cataagcgct
    21481 tccaccgcaa gtggtcgagc cctgcgcact gggcggcggg aaactagcgt accgaagaca
    21541 acggcccaca cgctcgctgc agtttgtttt gcagcccgtg caaaagcgcg tcactcggat
    21601 ctcccgcgcg tttgagcagc tcaccgagac tctgccggtc gttcttgccg agcggcgtct
    21661 tctgacgctg gcagatgcga agcagcgaat ccgcagcccc gcgacgcagc gcaagcggcg
    21721 catcccgatc gagccggccc atgagcaacg caaggtctac gtcgtcgcca tgttcaccca
    21781 gtgcgcgaat acaaagcagc gccgcgacca aacttcgatc cgcgccgtgt ggatcatcca
    21841 aaagatcgct cagtccgtcg ccaatcgccc ggtgtgcact cgccgccgaa tttgtaccaa
    21901 gtccaaaccg cgcgaccagc acacaagcct gaccacgcac cgacggcatg gttttttcat
    21961 ccaaaagcag cgactgataa cgcgaaattc cttgcgcccc gacgcagcgt gcaagtccag
    22021 cgagtgacgc cgccgccacg ctttcctctg catccgcgac cgcttgttcg agcgatgctt
    22081 gggccgtccc acccggttga cagagcgagc ccagcacttc ggcagcagcg cgacggacca
    22141 tgggccaaga atcccgcgcg agcagcggtg acaaaagccc gctcagcgcc tcgctttgtc
    22201 cgctcaccga gctgcgacca aacagcccaa ccagcagcgc cgtccgaacc ccggggtcgg
    22261 gatcagaaac cgcccgcttc acgccatcca gatccagcgt ccgactccgc ccgagcgccc
    22321 gcgccgccag cttgcgcaga tccggttctg tttcgttttg caaaaccgtc atgaccagcg
    22381 ggtctaccgt cggcaaatcc agctttgaca gcacggcaag cagccgatag cgcagcgcaa
    22441 accgcgtttc agcactcgcc tgcgacgcaa gctgcgacca aaatccaccc agtttttccg
    22501 ccagctgctg ccggctgtcg tcgttgggac agcccgactg tgccgccaga agtccctcgc
    22561 caagcagcac cagccacgcg ggatcgacat cggtgcgccg atattccgat aacgccgtcg
    22621 ccacgcgcgg atcttgcggc ccgacgcagt gcagcgctcg acccatcgcc gccgcaaacg
    22681 ccgtccgcag cccggtttcc cgtgtgagct tttggccaag ctgtagcgga tacggccacg
    22741 ccgaatgctc acaaagccag cccgctgctt cggcacgcaa aagcgacgac cgcttcccgt
    22801 cgagcagcag cagttccagt ttgccgaccg ccagttcgag cggctcgcga ctgcgggacg
    22861 ctgccgtcag cgccgaaaac agcgccggcc tctcctccac ttccgccgat tgcagcgtcg
    22921 ccatcaacac atcgatcagc gcggcctgat tctttccgcc catcaccgac gaccgctctg
    22981 ccaatgtgcc aagcagaccc tgaattcgcc gacgcgcact gcccttggcg gtcttttctg
    23041 ccgccgccag cgactgcaaa agtgactcga tttgctcggg tcgcatcgac tccagtcgac
    23101 cccgcagcgt ccgctccgcc gcttccgcac tgcgcgtgtc cgtcgatcca agctgcccaa
    23161 tcagcagcgc aactgcatct ccgccatcga gatccgagta caggttcaca tccccgagcg
    23221 cgctgtgttg acctgccttc gtgccaggct gttcgatcgt cagcgacaca caatctgtcg
    23281 ggacgggctg cggcagcacg atccacaccg gctttgccac acccgccgtc ggtatccgaa
    23341 agctctgttt cgatgacagc gtcaaaacaa gctgggctgg catgatccct tgcgcactgg
    23401 gcgccggcac gatccgcacc gctcgcagcg accgaccggc gctttcggca cgccacgtaa
    23461 aaaacgtgcc cgtcgcgtca tgcggacccc tccacaccgt ctgcggctgc ccgtcatcga
    23521 cctcacgcgg gggcgaaagc aggtcggctc gctcgatccc agcttgccgg ctgacggccc
    23581 acatccgata catcccgatt ggctgattta caaactcgct gggccccgtc ggacttgtgg
    23641 acagcggtgc cactttttct ggcttcgcaa gcagtcgatc cggcgcaggt tgccattttc
    23701 cgtccccatc agggaaaaac cgttccaaaa atagccgtcg ctccccgtcg cagcgttcga
    23761 ctgaaggcgc ggtctgatag cgaaacaact cgccatcaga gacctcgacg acgactgcgt
    23821 actctccgtc gctcccgacc ggcccgatcg gccccgaata caccggttca agcttgcctg
    23881 ctcgccagcg cgcccacaga gccacccgct ggcctttgcc ccgcagtcca gtcgcgacaa
    23941 cgagtgtttc cccgccgcgc agtttttcga tcgtcagcct gccgtctgaa agccgaggaa
    24001 cgcgatcacc aagcagaagc gccagccggc tgcccgcgcc cgtcccagcc gcaatcgcaa
    24061 gcgtgccagc ttcatcgagc gaaagcgttt ccatccgtcc gtctccgtcg agatcgaccg
    24121 aaaccgagac cggtggtgaa gcagcctgac tcgacgaagc ggcaagcact tgggcaggcc
    24181 ccgcacacca cacacacaga ccgactcccg cagcaatcgc ccaagaaccg ctcgcgagct
    24241 taacttctcg attgaatccg agtgaggaca taggccacag taatacaaag cagttgacca
    24301 acgtgacaag gtttgctact cactccggct tgctgtatca aagctgccct gccgatattt
    24361 ggaggcgttt tccgtgactt gcaccctgtt tggcgctgca accgctctgc agagcgaccc
    24421 gtcttccctg ctggcacaag cggctggcgg cggcggcatt gcagccatga gcaacatgct
    24481 tctgccggtc tttctgatcg gcatcttcta cttcatcctc attcggccac agcaaaagcg
    24541 gcagcgtgag cttgaagact ggcgcaagca gatcaaaaaa ggcgacgaga tcatcacctc
    24601 ggggggcctg tgggcacggg tttcgtcgtc tgaagataag agccgatacg tcaccgtcga
    24661 gctgcaggac aaagtccgag tgaaagtcct gcgcgaacac atcatcagca aggcgccgcc
    24721 cgcggatgtg tcagtggccg cggcagacga agccaagtag tcacgatgtc gtttcagtca
    24781 ccctgcgaat tgtctgcact gtcctgccga gggcggtcac tgtttgccct ctagttagcc
    24841 tgggagaagc tcacgcatgg agcggtcttt ttactaccgc gtagctacct actcgctcat
    24901 caccatcctc tcggtgatgg tccttttgcc gtcgcttgcg gactggacgc acaagaccga
    24961 tcggcttccc gactggttta aaaaagcgtt cactcgcaag atatcgctcg gcctcgactt
    25021 gcagggcggt ctgcacctcg tctacgaggt tcaggtcgac aaagcggtgt ccgataaggc
    25081 cgaccgcttg gctgcgcaaa tcgaggaaaa gctcgccaag gacaagaagg tcaaaggcgc
    25141 ccgcgtggtc cgcgagggcg acggcgctga ggcccgactg cgagtcaatt tccagaacaa
    25201 gagcgacgcc aaaaagctcg aaggcaactt cgagctggag ttcgccaagt acctgactcg
    25261 cgacggtcaa aacgagcaga ccggcgagac agtgtacaag atggattcgg actacatcga
    25321 cgagctgcgc agctatgcgg tcagccaggg tgtcgaaacg atcaaaaatc gcgtcgacaa
    25381 actcggtgtg tcggaacccg tcgtccagcg caagcagaac gacatcatcg tcgaactggc
    25441 cggtctatcg caggctgact tcgagcgcgt caagagcctg atcggcaaga ccgcgcagct
    25501 cgagttcaag atggcggacg atggtgccga gtacatgcag aagctatcgg cccatgccga
    25561 aagcaagaaa gcgcagtacc cgggtctcga cgtcggcttt gatgcctggc aacaaaaaga
    25621 caccggacag cagcacaccg acgtctacct gaaagcgaaa gacaagacgg tgatggagca
    25681 gttcctcaaa gagctgccgc cggagctagc ggttccaccg gatcaccaga tcctgtttga
    25741 gcagaccgca cgccgaaacg acgacggaga agagacgcaa gacaagctgt ggcggtcgta
    25801 ctacttgttc cgtcgtgccg agctgacggg tgagtatctg accgacgctg aagttcagtt
    25861 tgatcccaat acgggccgcc ccgaagtcgc gattaccttc gacagcgagg gtgccggtct
    25921 gttcgaaaaa gtctcgggtt caaacatcgg tcgtcggatg gcaatcatcc tcgacgaaaa
    25981 agtcagctca gcaccgacca tcgaaggacg tatcggcggt ggtcgagccc gcatcaccct
    26041 cggtggtttt tcggacccct accagcttca gcaggaagcc aaagacctcg tcgcggtgct
    26101 tcgcacgggt gcacttccgg cgccactgcg caagacgttc gaaacgcagg tcggtgcgac
    26161 actgggcccc gaggcggtct cgaaggcacg tacctcgatg atcatcggta cgctcgcggt
    26221 cgtcctcttc atgctgatct actaccgcgc cgccggcatc atcgccgaca ttgcgatggt
    26281 ctttaacgtc atctacctgc tcgcgatcat ggcgctcatc caagcgacgc tgacgctgcc
    26341 gggtatcgcc gccgtcgtgc ttacgatcgg tatggccgtc gacgccaaca tcatcttcta
    26401 cgaacgcatc cgcgaagaac tacgcagcgg caagacccca agacaagcgg tagaaaacgg
    26461 tttcggtcgc gcgttctgga cggtcttcga tgcccacgtc acaaacctgg tcgccggtat
    26521 cgtcctttat tcctacggaa cgggtccgat tcgcggcttt gcggtttcgc tgatggccgg
    26581 tatcgtctgc aacctgttta cgtcggtgtg gctgtcgcgt gtgatgtttg actttatggt
    26641 cgggcgcaac aaaaagcgcg ccaccctgag catctgaggc gcctatgtcc cagcaattct
    26701 tcgagcttat ccgcccagac cacaacttcg acttcgtcgg caagacgaag ccgctgctcc
    26761 tgtcatcggc agtgttgatc gtgctgtcgt tcctgatgtt gccgctcaac atggtgatcc
    26821 cgggacgagg acacgcgctg aacttcgcca tcgacttccg cggtggcacc gagatgcgtg
    26881 tcgacttcag ccgagagatt tctccgtcgg aagttcgcga cgcaatggaa accggtggct
    26941 acaaggacag cgaggtcgtt ccgctgcccg agttcaaaaa tgccttcctg gttcgtttcc
    27001 ctgcggtgtc ggcccttcag cccgagcagg tcaagcaagc cgaggcggcg ctgcgtggca
    27061 agctaggtca agaagccctg cgcaagttcg agttcagcga cggcggtgac aaagcttacg
    27121 tcaagctcga caagtcgaaa tcggtcgaac cggcccaaat cgacgaaatc ctgcgcagcg
    27181 tcaacatcaa gtcgaaccaa gtgcagcgct tcggtcgtcc cgaagacaac acgtttgaag
    27241 tcgttcttgt cggcctcgat cgcgaagtcc gcgccgcact cgacagcaag cttggtcaag
    27301 gtgccgtttc ggcgattccg tcggtcggat cagtgggtgc caaagccggc aaggagctgc
    27361 gcgacaacgg catcatctcg ctgctggccg cgatcggctt catcatgctg tacatcctgg
    27421 tccgcttcga tttccgctac ggaccgggca ccgtcatcgc actgctccac gacgcgatct
    27481 ttgttctggg cgccttcgcg ctgacctaca aagagttttc gctgacgacg atcgcagcca
    27541 tcctgacggt tatcggttac tcgatgaacg acaccatcgt cgtgttcgac cgcatccgtg
    27601 aaaacgcgag caagatgcgc gacaagaaat tcccgatgat cgtcaacttg tcggtaaatg
    27661 aaacgctgtc gcgaacgatc ctgacctcgg cgacggtgtt ctttgtgacg ttggcgatga
    27721 acatctgggg cactggcgtc attcgtgact ttgccttcgc gatgaacgtc ggtgtcatcg
    27781 tcggtacgta ctcgtcgatc tttatcgcga gcccgatcct gatttggctc aacgaaaagt
    27841 cgatgcagtc gcagaaaaac ccccgccgtt cgagcgctgt ttcgcccgtc tagccgcgcc
    27901 cgagccgcag tccggcgcac catgtccccc cgttcgctgc ccagcctttc cgaatttcag
    27961 cacagcggcc agttcgtcga ttttcgcggc caccgcatct ttgtccgcga cgccggccat
    28021 ggtccgccct tgctttgtct gcacggcttt ccgacgtcga gctacgactt tgcgcggatt
    28081 gtgccagagc ttgtcgcgca ccggcgggtg gtgctgttcg actttctcgg tttcggtttt
    28141 tccgacaagc cgcaacacca tccctactcg ctgttcgagc aagccgacat tgcccaagcg
    28201 gtggccaagg agctttctct cgggaagctg acgcttttgg cgcacgacat gggaagctcg
    28261 gtggcgctgg agcttttgcg acggagcacg ccggagatcg agcgcctggt gctgctcaac
    28321 ggcagcgtgt ttttgcgcca ctatcggcct cttttgtcgc agcgcttgct cctgcacaaa
    28381 caggtggggc cgcttctgac gagactgggg cttatcaacc ggcgggtgtt ccacgcgcag
    28441 tttgccaagc tgttccccga tcgcccgtcc gacgaagagt tggacctgtt ttggcagctg
    28501 attgtgcaca acggcggcca acgcaattac cacctgctga ttcgctatct cgatgaacga
    28561 caccagcacg agctaacttg gcttgatgcg ctgacggcgc accgcgcgcc gctgacggtg
    28621 atttggggac agcgcgatcc ggtgtcggtg ccagcgattg ccaaagaagt gctgctgcgc
    28681 cggcccgacg cgacgtatgt gccgctgtac gacatcggcc actatccgca gtgggaagat
    28741 ccgcagaccg tcgctcgcat cgtgctcggg ctgtaagcgc tacagccagc gtcttgcgat
    28801 cgcatcgcga attcgctgcg ccgccccacc atcggcgagt cgcagggtca gtgcctgagc
    28861 cgcagcgcgc atggccaaaa cttcgtgttc atcgagacag gccagcccgc gctgcccaat
    28921 gtccgcttcg cgattgccga tgagctttgc gcagccggcg tcgaccagat acatccactc
    28981 ggtttcgttg cgcaggacca gcaccggagt ccccagcgca tacgactctt cttgcagccc
    29041 gcccgaatcg gtacacagcg cccgcgcctg ctgcaaaagc gccagcatgt ccagatagcc
    29101 aaccggcggc aaaagccgga tgcgcggcgg caccacaagc tttcgctcgt cgatgattcg
    29161 ctgcgtgcgc ggatggacag gccacagaat cggatagcgt tcggcgagcc gtccgagccc
    29221 acgcaaaaca cccggcagca cgtcgggcgt ggtgttttcg gcgcgatgca cggtacaggc
    29281 caaaaacggc gtctgtggcg aaagccccag cgaaagcagc gcatctatga caacggttcg
    29341 gtgctgcgcc ttgtttgcaa acgcccgtgc cgcatcaatc gccgtcgaac caaccacggt
    29401 gatgtcggcg gcggaaattc cttcggacag gagctgcgtt ttggacgctt cgtttggcgc
    29461 cagcagctgc gcggcgcagt ggtcgacgag cacgcggttg atttcttcgg gcatcgtgcg
    29521 cacaaacgat cgacagcctg cctcgatgtg gaccacgggc accgccaact ttgccgccga
    29581 aagcgccgcc ccgagcgtgc tgttggtatc gccgagcacc agcaccagtc cgattcgttc
    29641 gcgctggatc gcctgttcca tcccgacgag gatcgccgcg gtctgcgccg catgcgtctg
    29701 cgatccgacc gccagctgca catcgacggg acgcagcccc agctcggcaa aaaacagcgc
    29761 atccatttca aaggagtagt gctgcccggt gtgcaaaagg atctgccgca tgcccggctg
    29821 ctcgtcgaga agcgggataa gcggggccag cttgatgatc tcgggacgag tgccgaggac
    29881 cgtgcagacc gtaagcgggt ttttggtcat catccgagct gcgccacttt ccgcatcatc
    29941 gcccgcggca aaagacccgc ttggaagcgc aggtgatcgg ccaaaatgcg cgccactctg
    30001 gctttcgact gaccgtactt gcgaacgtgc agcaccgtcg gaaattcgac cacgcggaag
    30061 ccggccaaaa gcgactcact gaggatttcc gccatcgcca gataaccggg cgaggaaaat
    30121 tggacttggt caagcacctt ccgtcgataa gcccgaaaca tcgcggtgta cgtgtgcaag
    30181 tttcgctcaa gcaagatgcg ataacagagc gacgcgccct gcgacaaaag cagcctgtag
    30241 gccggaactc cgtcgacacc gccttcggga tgatacggcg acgccgtcac cacatcgaca
    30301 tccggcaaca gcagcgcaca caggcccaag atttcgccaa actcatacgt tccgtcggcg
    30361 tccgtcgtca ccaccacatc gctgcggctg tgggcaaaac cggttcgcag agccgcccca
    30421 agcccgcgat tttgctcgtg ggacagcacg caaaagcgcg gatcggaccc aaaacattcc
    30481 cgcagaagcc gcggcgtcgc atcggtcgat ccatcgtcga cgagcagcac tttcacatcc
    30541 gcgtagcggc tcgccagttt gtcgagcacc gcgggcagcc gagcacagag ctgcggcagc
    30601 gcgtcttctt cgttaaagca cggaatgacg atcgacagcg tttcgcgcga tgaaagttcg
    30661 gacacgcccg cattttacgg caaccgtgcc gccaggtatc gacacagaag ccacctgttt
    30721 tttcccaagt tacttgggaa cgagcgccgg cagaacgcgc tgggcctgtt cgggaaccat
    30781 gcgaagcaaa aaactgtccg ccgtcgccgt cgcgatcact tggcttgtcg cgggccgatc
    30841 cgtcgatgaa agcaagcgat tcaccgtgtc gacagcaagg ccgcggccca gatcgtcgac
    30901 gaaaaatacc ggtggttggg actgcccagg cggaatcgcc agcggcgaca aatggagcag
    30961 gcgcagcgca gtttccgaca gctctgacgg ggaaaaatca ctttcgcgcc cacttggacc
    31021 ccgcagtccg acttgcagcg acgtcaaaaa cagatcgacg aagttgcctt cgcgttcggc
    31081 aatcagctca ccgtcgggcg tctggacaaa cgcatcggga tagccaagca gcgctgaaac
    31141 cacctgtcgc tgctcctcct cgttttcaaa gtagtcgggc ccaatcggcg tcttgcgcgc
    31201 cgcaacatgg gtcacaggca acccggaccc acgcagcagc tcggcgagct tttcggcaaa
    31261 cgccggatga tgcgccgccc gcgctgaaag acccgtcacc cgcggctccg gtcgaatcag
    31321 ctgaagctgt tcagaaagcc aggccccaaa cggggccgcg acctgcgaac cttcggccag
    31381 tgccgccgcg aacagtggct gttccgcccc caaatgcacc gcgagcgact cccacagccc
    31441 gcgctcagac cgggcacccg gccccacttt tacgtcggtc agctgcaaga tcggattttt
    31501 ccgttcgcgc cgaaacacaa gctgctccgg ctcgccacgg acgcacacgc tcagcgattc
    31561 ggcatcgact tgctcgggac gcagcgccag ctcgtagcga tacagcgcat cttgcgcgaa
    31621 cacctcgatt gcgaacgcga catcggaccg cggtggtgag gcaagccgac agaccggcag
    31681 gcttgcgcgc tgtcccggct tttgaccatg cagcgcaagc tgccgaagct gcgccagcgc
    31741 tttgagcaag ttcgatttcc ccgtcgctgc atcgcccacg atcagcgcca gcggcagcac
    31801 ccgaaaacca tcgggcaaga ttggtcccaa aggcgcactt tccgtccctt ccggcgctcg
    31861 caaatcgagc tgccagcgcg gaccaaacga cagaaagttc tccacggcga agcggacaag
    31921 catgggagcg cgactttgcg caagcgacgc tttggggtca agcacttgtc gctctggggt
    31981 agctctgtta ccgtccggtc tgaaggagtt tttatcgcat gcgcaaactg ctgtggagtg
    32041 cggccctgtc cttggctgtg gcctcgtcgg cctgctcaaa tgaaacggcg gtcctttctc
    32101 tcgacccgcc gcagggaacg tactcgggcg gcgaagagat cgtgatcaaa ggccgcaact
    32161 ttccgacggg ccgaggcggt gtcagcgtcc tgttcggtcg tcacgcggcc accaacgtga
    32221 tcctcgaaag cgccagcgcg attcgcgtca catccccggc gggcgatcgc aacaccaagg
    32281 ccgacatcgt ggtcatgttc gacgatggcc gcgcctacca gctcaaggat gcgtttcagt
    32341 atctcgacgc gacggacaac agcaaggtga tgaagcactt cggcaacaag aagtagctcc
    32401 gcggcggttt ttggctagac tgcgcccgca tgtctagcgc gatggatcga ttgatttcgg
    32461 cggacgaaat ccgcgtgacc gccgaaatgg cgaatcttac gcttcccgaa caagaaattc
    32521 ccaagctggt cgacgagctg tcggcgatct tgggttacgc gcagtcgctt ttggccgtcg
    32581 acgtgactgg ggttccgcca atgacccatg cggtgccgtc gcactgtccg ctgcgtggcg
    32641 atgtcgtcct tgcgcacgac tcgacagact ccgcgatgcg caatgccccc gccaaggaag
    32701 catcctattt tgcagttccg gcggtgtttg cgggcggcga cgacggattt gatgcggagg
    32761 gctaagcgat gacggcgcaa cagtctgtcg gtgattcgtt cggttcggcg ctggaactgg
    32821 cccaaaaagt ccgctcgcgt gaggtgtctg ccgttgaagt aacccgcagc tacctccagc
    32881 gcatccgccg gttcgacgaa gcgcttggct gttttttgtg cgtcgatgaa aaaggggcgc
    32941 tcgccgcagc ccaagccgtc gatgaaaaag ttcgcgacgg tgccgacgaa gagcttgtgc
    33001 tggccggcgt gccggtcggc atcaaggaca tgctgtgtac agagggactg gccacgacgg
    33061 cgggatcgaa aatcctggcc gggtttgtgc cgccttacga tgcgacggtg gtggccaagc
    33121 tgcgcgacgc gggcgcgatc atcattggca agtgcaatct cgacgagttt gcgatgggct
    33181 cgtcgaatga aaattcggcg tataagccgt gcaaaaatcc ctgggatctg acgcgggtgc
    33241 caggcggcag ctcggggggt tcggcagcag cggtggcggc gtcgctgtgt ccgatcagcc
    33301 tgggcaccga taccggcggt tcgattcgcc agccagcggc gctgtgcggc atcaccggga
    33361 tgaagccgac gtatggccgg gtctcgcgtt atggcgtgat tgcgtttgcc agctcgctcg
    33421 atcaggtggg gccgctcgga acaagtgcaa tcgacgtcgc acagacgctg caagtgatcg
    33481 gcggccacga cagccacgac gcaaccagct tggcgatgga acagcccgat tatttggccg
    33541 cgtgtcaaag accgctcgtc gacaaaggcc ggcgcgtgcg gattggcgtg cccaaagaat
    33601 acttcgccaa agggctcgat ccgcggattg aacgctgtgt gcgcgatgcc atggctcgtc
    33661 tggaacgtga cggcgccgag ctggtcgatg tgtcgctgcc tctcaccgag catgcggtgg
    33721 cgatttatta cctggtggca accgccgaag cgtcgagcaa tctgtcgcgt tacgacggtg
    33781 tgcgctacgg cttgcgcgtc acagcgaaag aaggcgagcc actgtccggt ccgtcagcgc
    33841 tcgtcgagat gtacaaacga acgcgcgcgg ctggttttgg tccagaagtc cgccgtcgcg
    33901 tcatgctcgg gacgtatgtg ctgcgatcgg gctattacga tgcctattat cgtcgcgcaa
    33961 gccaggtgcg gacgctggtg gcgcgtgatt ttgccgcggc gtttgcgcgc tgtgatgtgc
    34021 tgctcacgcc gacgtcgccg gtgctgccgt ttcggctggg tgagcgcctg cacgatccgc
    34081 tggcgatgta tctatcggac atctacacga ttggctgcaa cttggcgggg ctgccggggc
    34141 tgtcgatgcc gtgtggtttc gtctgtgaac cgggtgatga aaagccactt cccgtcgggt
    34201 tgcaaatcct cgggccggcg ctccacgacg ccgaagtgct gcgcgtggcg gcggcgtatc
    34261 aacggctgac cgactggcat acccgcaggc cgaccgcatt tttggcaaaa aaggagcccg
    34321 cgtaacatgg cgtctcccgt cgagtatgag ccaatcatcg gcctggaagt ccacgtccaa
    34381 ctcagcacgc tgagcaaagc gttctgtggg gccaaagcgg cgtttggtgg ggcccccaac
    34441 tcgtacatcg atccgtacac gctcgggctg cccggcgcgc tgccggttct caatcaccgg
    34501 gcggtcgagt tcgcgctgcg catgggtctg gcctgccagt gcgacattgc gcttcgtagt
    34561 gagctttcgc gcaaacacta cttttacccc gactcgccga agggttatca gatcagccaa
    34621 tttgacaagc cgatctgtga aaacggccac gtcgagtttg tgctgcgcgg cgaaaaaaag
    34681 cgcgtcggac tccagcgcat ccacatcgag gaagacgccg gcaaaaacct ccacctgccg
    34741 ggcggacgaa cgtcgctggt cgatttcaac cgcgccggag tgccgctcat cgaagtggtg
    34801 tcgctgcccg aaattcgttc ggcggaagaa gcggcagaat atctgcgagc cctgcgtcaa
    34861 ctggtccgtt ttctgggaat ttccgacgga aatatggaag aaggatcgct gcgctgcgat
    34921 gccaacgtgt cgattcgtcc acgcggccaa acgacgctgg gccagcgcac cgagctgaaa
    34981 aacatcaact cgtttaagta cgtccagcac gcgattgagc acgaaatcct gcgccaaatc
    35041 gacgtcgttc agtcgggcgg caaggtggtg atggaaaccc gcggctggga cgcggcgcgc
    35101 ggcacgtcac ggagtcagcg caaaaaagaa caagcgcacg actatcgcta catgcccgac
    35161 ccggatctgc cgccgcttca tatcgatccg ctgtggcttg attcgatccg cgcagcgctg
    35221 ccagcaacgc cgatgcagcg tcgggatcgc tacatcgatc ggctggggct gtcgccctat
    35281 gatgcatcgg tgctgacggc agagcgcgag ctgtgtgatt tttttgatgc cgcgctggtc
    35341 gcgtcgggcg tgtccacgga cagtgaatcg gtcagcgaag cgcagtgcag ccgggccaag
    35401 ctgtgtgcga actggctgtc gtcggagctt ttggggctgc tcaacaaaga aagccgcgac
    35461 atcaccgcgt cgcccgtttc tgccgaatct ctcggcgagc ttgtgcgcta catttccgac
    35521 gacatcatca gcggcaagca gggcaaagag gtcttttcgc ggatgtacga aagccgggaa
    35581 accccgacgg acatcatcga aagactgggg ctgcgtcaga tcaccgacga agccgtgatc
    35641 actgccgcct gccgcaaagt cgtcgccgac ccggcgcttg cgaaacagat cgaaaagtac
    35701 aaacaaaatc ccaagctgct cggtttcttc gtcggaaaag cgctcgcgga aacgcaaggc
    35761 aaggccaaac ccgatctgct ttccacgatc atgcagcgcg ttctgagcga gaaatagcca
    35821 tgtccgcttc tttgcaccct acccttttgt cgccgctgcg ctttgataag ggcctttgcg
    35881 agctcttgct catcgaccag ctggcactgc ccggcgaaga aatctggcgc cgctacagcg
    35941 actaccaagc ggtggccgat gcgatccgca acatggtcgt ccggggcgcg cccgcgattg
    36001 gcattgcggc agcgtttggc gtcgtgctcg gcgtcaaagc cggtgagccg ctcgatcagg
    36061 tgattgcgac gctgcgcagc actcgtccga cggcggtgaa cctgttttgg gcgctcgacc
    36121 ggatgcagcg ccgggccgcc gagctgtcga tcctgtcaca ccacaaacaa gtcgaaggcc
    36181 tgtgcaccga agcgcaagcc atatgggacg aagatgtcgc gatgtgtctg cgaatggcag
    36241 agcatggcgc aaagctactg ccacccggaa acgtcctcac acactgcaat gccgggggct
    36301 tggcgaccgg tggatatgga accgccgtcg gagtgattcg cagtgcgttt gcacttggac
    36361 acattgccgg cgtctttgcc gacgaaacgc gaccgttttt gcaaggcgct cgtctgaccg
    36421 cgtatgaatt tcaaaaagcc ggcgtgcccg tcacggtcat ctgcgacaac atggccggcc
    36481 acctgatggc gcgcggcgaa atccagagcg tcatcgtcgg tgccgaccgc attgccaaaa
    36541 acggcgatgt cgccaacaaa atcggcacct actcgctggc cgtgctcgcc aaagcccatg
    36601 gcatcccgtt tttcgtcgcg gcaccgcgct cgacgcttga tccgaaaacg gcgtcgggtc
    36661 gcgacatccc gatcgaagaa cgcagcaccg aagaggtcgt caaaatcggt cccgttcgct
    36721 tggcgccggt cggagtactg gctcgacatc cggcctttga cgtcacgcca gcacatcttg
    36781 tcactgcaat catcacggat gctggcatcg caagggctcc ctatgatacg agcctgtctg
    36841 cgcttctttt ggccacatag acagaaagtc ctttggcctc aaactcggaa atcctgcaaa
    36901 atcaaaccag agaggaccgg catccgttgg ccgcgttctt cgtcgtgagc cgataggtaa
    36961 acggggaggg tgtcttggag aaactaaagg ctggagggga aacctccgca tactgcacgt
    37021 catgtaagga catgcgccac catgtggttg ttgccatgga cggcgacgtg cctgccaaag
    37081 tggaatgtct cagctgccac aagcagcaca agttcaaggc tgcaccgcct ggcaccaagg
    37141 tcgccaaagc ggctgcggga gggacgaagg ctccgcgggc caagtcggcg acgcgaccgg
    37201 cggcgcactc ggtgccggtg gtcacgggac ccaatccgct ggatacgatc ttgcaaacgc
    37261 gcggcatcgg cagtgcccgc agctactcgc ccgcagaacg ctacgccgtg ggtgagctgg
    37321 tgtcgcaccc caatttcggg atcggcgccg tgacggccac gccttcgccg ggcaagatgt
    37381 cggtgctgtt ccgtgacacg acgcggctgc ttttgcacga acgcacgccc gtggcggtgg
    37441 ccccggtggc caagctcggc ccgccgcctc gtcgggagga gtcgccgacc ggtcccgccg
    37501 atcggccccc gaaagtaaag ccgcttttgt aagatcggtt ttttggtctg cgagccaccg
    37561 cgtcacacaa gccgtgctag gctgtggcgc tggtgagcat cgcgacgcgg gttacgcttc
    37621 tgacggtcct tctggtgacg ctggcactcg gcgtctacgg gactcttagt atgcgcggtc
    37681 gtcgggccga acgaaccgcc gagcttgagc ggcagacgtg gctgtttggc tcggcgctca
    37741 aggtcgcgct cgaggccgcg gcgcaagacg gtctgtacga agacttgcat gggctgatcg
    37801 gtcgcatgca agccgccgag cgcaccatcg aggtgacgta ccaagacctc ttgcagcccg
    37861 gcgaagacgc ggtgtttccg cccggtagca gcgcaaggct ggtacggcag ccaaaccccg
    37921 acgcaggacg ggaagaacgc cgtcgccgcg tcgaaatcac ccgccaaccc tacggcgaac
    37981 acatctacat aagcggtcgc gaggtctacg cctattcgct gccgatcgtc gacaacaaac
    38041 accgcatggt cgcgatcatc gacctccagc gcgacaaaag cgagctggaa caagagatgc
    38101 tgcgggcgac gcgcgatgtg gtgctgacgc tcgggggttt ggccgcgctg ctggcgattt
    38161 tggtcagtct gacggtccgg caagagtttt cggcgccgct tttgcggctc gtcggtggca
    38221 tcgacgaagt gacgcggggc gactacacag gtgcgatctt gcgagagcgc aacgacgaag
    38281 tggggctttt ggccgatcgc ttcaacgaaa tgacgcagtc gctgcgggaa gcacgcaatg
    38341 aaatcctggc cggtgtcgac gccaagctgc agctggaaca gaggctgcgg cattccgaca
    38401 agctagcaac catcggacag cttgcggcca acattgcgca cgaagtcggg acgccgctga
    38461 acgtcattgg cggtcgcgcc cgcgtcatgt cccgtcgcgc cgaagatccg acggcagtgc
    38521 aaaaaaacgc cgacatcatt gcgacgcagg ccgaacggat caccaagatc atccagcagc
    38581 tgctcgacta tgcgcgacgg aaagcaccgg cccgcagcga cgtcgatttg cgcaaagtat
    38641 gccttagcac gctcgatttt ttggaagtgc agctcaccac tcggcacgtc gcggcgcagc
    38701 ttcatccgtt tgcggtgccg acgcccccac agccagaaag ccaaatcgaa cgagcggtgc
    38761 gcggcgatga atcgctcggt acactcaagc cgcccgccca gccgattgtc gtcggtgatt
    38821 ccgatcaact acagcaggtg tgcctgaacc tgtgcatcaa cgcgattcaa gcgatgcccg
    38881 aaggaggcgc gcttgatctg tcgctggaag gcattgttcg tcgcaaacca gggctcgaca
    38941 aggcgccacc gggcgcttac gtcatgctgg ccgtcgccga caccggcatt ggcatcgacg
    39001 aggccgatct gagccgcatt tttgaaccgt tttattcgac caagaccgac ggaaaaaccg
    39061 gcaaagatgg accttcgggc agtggtctgg gtctgtcggt ttccgccggc attgtcaaag
    39121 acctcgacgg ctggatcgag tgtgaacgac gcccgacggg aggcacgatc ttccgggtct
    39181 ttttgccggc gcccgagcga gaagaaccaa acacccgtcc gcagcgcagc cgtccacccg
    39241 cccgccaaaa gcagcaaagc accgtgccgg agagcgaggg caattcgttt gaagctgcgc
    39301 ctgcggacgt gttaagccaa attgagacgg aacactgatg gcccagccac ccaagacgac
    39361 cagcaagctg ccgcaggtgc cgctcgcggc gggaaaaagc ggcccgcgat cccaaccacc
    39421 accgctgccg agtgatacga gcagcgatcg ggccagccgt catacgattt tggtcgtcga
    39481 agacgatgcc gcgatgcgtg atctgctcga agattcgcta cgcgacgaag gctatcgtgt
    39541 cgaaacggca gccggcggtc atgctggcgt cgcacgggtg caaaaaggcg gcatcgatct
    39601 ggtcgtcacc gacgtaagaa tgccggatct cgacggactg gacatgctgc gcgagatcaa
    39661 gagtgcgccg ctcgaacagc cgccgcatgt catcgtcatc accgcgtttg gatcgatcga
    39721 caccgccatc aaagccgtcc ggctcggcgc gcacgactac atcaccaagc cgtttaacat
    39781 ggaaacgctg tttcttgccg tcgagaaagc gctggaagag cgcggtctgc ggcaggaagt
    39841 ttcgcggctg cgacgggaag tcgagcgtcc atatcagttt gacaacatca tcggtcgctc
    39901 ggcgccgatg caggaagtgt ttgggctgat tcggcggctg tcgggttcgt cggtgaacgt
    39961 gctgatcacg ggcgaaagcg ggacgggcaa agaactggtc gcacgggcga ttcactatca
    40021 ctcgtcgcgg gccaaacagc cgttcgtcgc ggtaaactgc gcggcgattc ccgatcagct
    40081 tctcgaatcg gagctgtttg gctacaagcg cggcgcattt accgacgcgc gcagcgatcg
    40141 ctcgggcctg tttgtcgaag ccgatcaagg cacgctgttt ctcgatgaaa ttggcgatct
    40201 gtcaccggcg ctgcaggcca agctgcttcg tgttttggcc gagcgcgaga ttcgaccgct
    40261 gggttcgtcg cggcctgaaa aagtcgacgt gcgggtgatt tcagcgacgc acaaggatct
    40321 gagccagggc atgcgcgccg gttcgttccg cgaagacctt tattaccgtc tgaatgtgct
    40381 gcaaatccag cttccgccgc tgcgtgatcg cgccgaagac attctgccgc tcgccgatca
    40441 ctttttggcc aaagcgcgcg agcggggcgg caaacgagtg acggcgattc atccacaggc
    40501 aaccaagctg cttctgtcgc atagctggcc cggtaacgtc cgcgagctgg aaaacagcat
    40561 cgagcgagcg gtggcgctgt gtgagagctc gcaggtgatg gcgcatgatc tgccgccgca
    40621 gctgcgcgaa aaacggcgtg aggacgtgat cgcctacgcg ctgtcccgca acatgacgct
    40681 ggaggagctg gagcgcgaat acatcttgcg ggtgatgaag gaagagagcg gcaacaagac
    40741 gcgggcagcg cagcggctcg ggctcgatcg caagacgctg tatcgcaagc ttgaggaagc
    40801 cggcaagcag acggcgggcg acggggatgg tgacggagag acggacaatg gctcagccga
    40861 cgaccacgac tagcgatccg ctgccgcgtg gcttccgcag gctgatcctt gcgtactacg
    40921 tgacgatccg cgtcattttg tcgtactact ttttgcgttt ccgtctgcga atcgccgggc
    40981 ccgacgctgc aagtcggctg ctgatggatg cgcatcgcaa aaatgcccga cggattcagg
    41041 tcgcgatcga acggctcggc gggctgttta tcaaagtggg tcagctcatc agcatcatga
    41101 ccaacgtgct gcccgagccg ttccgcgccc aactggaaac cctgcaagat cgggtgccgg
    41161 cccgtcccta cgccgacatt gaacagcgct tcaccgaaga atttgccggt cggcgcccgt
    41221 cggaagtgtt cgcatcgttt tcgcccgaac ccgtcgcatc agcgtcgatc ggccaggttc
    41281 accgcgcccg cacccaagac ggtcatgacg tcgcggtgaa agtgcagtac cccgacatca
    41341 acgaaatcgt ccgcattgat ctcgcagcgc tcggacggat tttttggctt ctgcattgga
    41401 ttgcgcccgc gcacggactc gacgctgtgt ttgtcgaaat tcgcgcgatg gtgatgcaag
    41461 agctcgattt ttccgccgag gcccacaacc tgtcgcggat ttcggaaaat ttcggtcgct
    41521 ataagccgcc gctgccggtc gatttcccca aagtcattgc cgagctatcg acgccgcggg
    41581 tgctgacgac gacgtggata gaaggtgtca aagtatcgaa tctttcgcgg ctcgacgagc
    41641 tgggtgtcga tcgcgacaaa ctggcccgca ccgtcgtgac agcgtactgc cagcagattt
    41701 ttcgcgacgg gctttatcac gccgatccgc acccaggaaa tctgcttgtg ctgccggccc
    41761 ccaaaccgag cggcgaaggc gatgcggcgc atgcaaacga tgtggccagt gtgccgacgc
    41821 cgtggctcga tctgggtggc gccaagctgg tgtttttgga ctttggcgcg gtcgccgagc
    41881 tatcgccgac gatgcggcgc ggaattgtcg acgtcctgca agcagcgctg acccgtgaca
    41941 cccagcgact gatcgtcgcg atgaaagaaa tgggctttat cgcccgcggt gccgatccac
    42001 agattttcga gcgcgtcgtc gacttttttc acgacaagtt tcaggccgaa ttcaaactgg
    42061 attccctgtc gctgagcgaa gtaaagctgt cgccggaacg cgcgcttgcg gatctggcgg
    42121 accttcgcaa gctcgacgtc aacttgcgcg acctgatgga gcacttccac gtccccaaag
    42181 agtggattct cctcgaacga acgctgcttc tgctgctggg actttgtacc gacctttcgc
    42241 ccgagctaaa cccgatgcag gtgatacggc cctacgtcga ggaaatggtg cttgggcagg
    42301 atcgcgactg gtcgaagctg ctcgtcgaaa ccacccgcga cgtggtgacg caggcggccc
    42361 agctgccggg tgagctgcgc aagttcctaa accgtgccca gcgcggccaa atcgaggtcc
    42421 gtttccgagg ccaagaagac accgcgatcc tgatccacgc ggtggcgcgc cagcttatcg
    42481 tgacggcgct tgggatcacc gcgtttgtga cctggctttc tctcgagggc cgcggacaat
    42541 ttgaacaagc cgactacgcg ctttacaccg ccgggacgct gtgcgtgtat cttttgtggc
    42601 tgatgctgac gacaccgctg ccccgtcggc gacggtagac tctgctatcg gaagcgcttt
    42661 ggcggctcga ctttcggaag gatcggcaag ccccgaacgc gagactggcg cttccccacg
    42721 ttccccgata gcattcgcag actatgaagt ttgcccatct cgcgctgtgc tcgcttttgc
    42781 ttgggccgtg ggctcatgca gacatcgcgt cgccccaacc gaaaattgcc gtgccgacgc
    42841 cgcacaaggc acaacctgcc aaaccgcagc cgaagcagcc gcaaccccag gtgccagcga
    42901 cgacagacag tccgcgcgat ccggtcagcg tggcccgcaa aacgcccgcc atcgccgaac
    42961 gaattgccaa gctcaaaggc gcctcggcgg gactttacaa tgaccaggcg ggcgcgcagc
    43021 tcgacgaaaa agtcacagtg atcgaactgg atggtcagtg cggttttgcc ggctgttcgt
    43081 cgacgtcgct ggttgttttc acctaccgta cccgcggtgc caacacagca acatcgtcgg
    43141 tgatggcgat ggtttcgtgt ccgccgatca tgcaaaaagg ttgcaccgcc gctgtcgcgg
    43201 aagtgcggcc ccaaggacag ctcgaacaaa cccgctgagt gagaatgcct ccgtctcgaa
    43261 cgcaggttgt agagccgctc tctccgcttc cttccgcttt ttcggcttcg ctgtttgtgg
    43321 ccggtgtgcc tgggccgtgg acccacgtcg tggtccgcac cttggaacaa gcgggatggg
    43381 acggcgtcgt gtatctgccg accgtgaatc tgtcttccgt cgcagacagc gggaaacgca
    43441 gcgaacttgt gcgctggcgc gatgaagcgc tgcggcacag tgacggcgtg ctgatctggt
    43501 tgggcgacgg cggaaaggac gacccgtggc tttttgagct gtggggtgcc tggcagcgct
    43561 cgtcgcgggt ggtgctcggc gtgccgcgtg atcagccggt gcccgactcc gccagccgtc
    43621 tgcacatccc catcgcccgc agtcccgtcg atgccgtcga gcaagcgtta cagatgctac
    43681 gcccaggtca gcagcgtcgg ggtggcgagc gcacagtgcc gctgcttttg tggcgcagtc
    43741 ccggttttgc cgcgtggtat caagcgcagc gcaaagctgg gcaccgactg gaagacgcgc
    43801 agcttgagtg gtcgtatcgc agccgcgccg ccggtcgtcc accgcttttg tgggcgctgc
    43861 gtccgcgggt gtttgtcgtt ggtgaacgcc ggcacaaaag cggcgaagtg gtgatcggac
    43921 gcaccgacgt aagcgcgagc gtcctttttt cccccgccaa aagtggcaac atcttcgacg
    43981 ccaaagtcgt gttggtgcgc gaatatcgct ccgcagtgcg caaccgactc ggctttggtt
    44041 tgatgctgcc tggcggttcc gcggcgatgg cgagcgagcg gaacacagac cccagacaga
    44101 ccgcgcagcg cgaagtcgcg gaagagaccg gtctgcaaat cgaccccgcg cagttcgagc
    44161 ccgtcgaagc cggggatcgc caagtcgccg cgtcgctcgc gagctatcac tgccacctgt
    44221 ttcgcgtcac gttgcgcagc gatcagctgc aagcccttga agcaacggcg gcgtcgggaa
    44281 aatcgaacgg tgcgaacatt ggggaacgct gttacgtgac ggtcaagacc gtgcgtgagc
    44341 tgctcgattc gtccgatctc gactggagcc agcttgggat ggtgctgcac gcgctcggtg
    44401 tgcccccgcc aacgtgaccc cgttcggcct tttgccacac tgcccacgag gcgcgctaac
    44461 atgcccgcca tgagcacacc cgtttcgacc ccaaacgtcc agctttccgg caaagtggcc
    44521 atcgtcaccg gcgcgagccg cggcatcggt gaagccatcg ccaagagttt cgcccatcac
    44581 ggtgccaaag tggtcctggc ttcccgcaag ctcgatggac tcaaccgcgt cgccgacgcc
    44641 atccgccaag ctggtggcga cgccatcgcc atcgcctgtc acaccggcca gcccgatcaa
    44701 gtcgaaaagc tggtgtccga caccgtcgcc cactttggca aagtcgacat cctggtcaac
    44761 aacgccgcca ccaatcccta ttttggcccg atgctcagcg tcgactggtc ggcctgggac
    44821 aagacgttcg acgtcaacgt caaaggctac tttgccgcga cccgtgccgt cgcccagcac
    44881 ctgatcggaa gaaacgcccc cggttcgatt gtcaacgtcg ccagcgtcgt cggtttcatt
    44941 ggctcgccga tgcagggcgt ctacgcgatg accaaagccg ccgtcatcag catgaccaag
    45001 acgctcgcga tggagcttgg caccgccgga gtgcgcgtaa acgccatcgc ccccggactt
    45061 gtcgacacca agttcgcgtc ggcgctgacc caaaatgaag aaatcctcaa catggttctc
    45121 cagcgcacgg cactcaagcg cgtcggtcag cccgatgaca tcgccggcgc cgccctgtat
    45181 cttgcgagcg atgcttcgca atatttgacc ggccacacca tggtcgtcga cggcggctgg
    45241 acagtcgcat aagcttgcga aacccaaact gagcacgctt gtaagagcgc gacaacgctc
    45301 ggtgcaccac tgcgatagaa tggtcgcatg ccgcttgctc gatttggttc gcttcggccc
    45361 gtgtgcgcgg tcgtggcgct gtcgctggct gtccacggct gcgctgccga agccgtaagc
    45421 ttgagcagcg agtccgatcc cgccgggacg agctaccaaa agtcgctcga tcgctggagc
    45481 cgcgtcggtc gcgtcatctc gaaaaccgag tttgacaccc agattgtggt cgttgcgacg
    45541 ctgcggtcca agtcgttcca gcgcgcctac gtcgacaagt acctgcagac ctatgcaatt
    45601 gtcgatcgct gggaacgagc tcgcgtcgaa aaagagcaag ccgagcagac ccaaggcggc
    45661 ctctcgatgt tcattcgatc gacgacgcac accgcaagct ggaatgatct gcgaccacag
    45721 gcgtcacgct ggcggctgtc gctgctcgac gatcaagggc gcgaagtgca ggcggaccgg
    45781 gtggaaccgc tcaccagccg ccagaccgtc gagcagacct tgctaaccgg ccagagcgat
    45841 gcgtttacca agctgtggac tgttcatttt ccagaaaaag ccagcgacgg acagccgttt
    45901 ccgctcccag gcgcacgcca gatcagcttg cgcgtcgccg gtccgcttgg acaaaccgaa
    45961 ttgccatggc ttttgcaata gcgcacactc gctgcgcggt cgccgctcgg caccgctgta
    46021 aggtacgccc atgatcgcgc tcgaggttct gtcaggtagc ctgcgcggac agcgcttttt
    46081 gattcgagaa gccagcttca cgattggccg cgctccccac aacaacatcg tgctccctga
    46141 cgttcatttg tcgagtgagc acgcccagat tttccgcgaa gacgacaagt tcatcttcaa
    46201 ggatctgcgt tcgaccaacg gttcacggat tcagcgccgc gacggagccg ttcattacgt
    46261 tgatgcaacc acaggttttg aatcggtggt ctgcgaaggc gatcggctgc tgcttgggga
    46321 tccgctcgat ccggtgattc tgggttgcgt gctgtcggac aaccagcttg ccccgatgac
    46381 ggtgccgcca ggcggcagtt caccgacggt gcctgcgctc cagcttggtc gtgctgcgcc
    46441 ggcgccgcca agtggaccag ccaagccgag cgaaggcgaa tcgacggaac cggaaggccg
    46501 cgtgctggcg cgaaggccgc tgaccgaagc cgaagcagtc gccggcaagg ttgaacgcga
    46561 tccggttttg gcgacggggc tgctcgcgct gtccaagcga atgggccgcc gcgggctcga
    46621 tttacaagcc gttttcgaag gcatcgccga atcggtgttc gagctggtgc cgctgtcgac
    46681 ccacatcgcg atcgagctga ccgacggcag cgaaggaaaa atggcgacgg tgtttgggca
    46741 gacgcgcaaa agcgatccca aagccgatgc gacaaaagac cccaaacgcg agccagtgac
    46801 cgacggaaaa cccgcgctgc cgattcgggc atcgcgagcc gtaattcggc gcgtgctgtc
    46861 ggaacgagcg gcggtgctca tcgccaatgc gccacaggat ttggctggcg cagcgtcgat
    46921 catgggcgcg cgcattcagt cgatcatcgg cgttcccctg tgggacggcg acgaaattcg
    46981 cggcgtgatc cagtgtgaca accgcagctc ggcggcgatg tttcgggaac gcgatctcga
    47041 agtgctgctc gtgctggctg gtcaagcgtc gctggcgatc gaaaatgcgc gacttcatca
    47101 gcgactgcgg ctcgccgaag aacagctccg ggcggaaaat cgctatctaa aaagccgcga
    47161 ggaaaagcgt cgcaccgtcg agccgatcgg ccaaagtccc gccttccgcg aggtgttaaa
    47221 gcaagttcag aaagtcacgg acacccgcgc caccgtctgc atcgaaggcg aaactggcac
    47281 cggcaaagag cttgtcgctt cgctgattca tgggcagtcg agccgccgcg acaagctgtt
    47341 cgtcgcccaa aactgcgccg ccgttcctga cacgctgctc gaatccgagc tgtttggtca
    47401 caaaaaaggt gcgtttaccg gcgcagacgc cgacaaaaaa ggtctgtttg aagtcgccga
    47461 cggtggcacg ctgtttctcg atgaaattgg cgaaatgtcg ctcgggctgc aagccaagct
    47521 tttgcgcgtg ctgcaagaaa gcgaagtccg tcccgtcggt gcgacccagc cacgcaaagt
    47581 cgatgtgcgg attctttgcg cgaccaaccg gtcgctcgaa aaagaggttg ccgaaggtcg
    47641 tttccgtcag gatctctact atcggttgaa ggtctatccg attcgcttgc cggccctgcg
    47701 tgagcgacgg gaagacattg ggctcctcgc agaacacttt ttgcaaaagt actcgagcga
    47761 gatgaaaaag ccggtggctg gttttacgtc ggaagcgctg gcacagatgg cggcctacaa
    47821 ctggccgggc aacgtgcgcg agctggaaaa tgaagtacag cggctggtga ttcaggtcga
    47881 gcccgaagcg ttcatcacgc cggatctgct cgcgccacgg atgcgtcaag ccgaagggct
    47941 gatggatcgg attgcgccgc aaaaaggcca gctcaaagac atgatggaag aagttgagcg
    48001 atttttgctc attcaggcgc tcaaagacca tggcggcaac aagacgcgca ccgccgagac
    48061 gctggggatc acgcgcgaag gcctgcacaa aaaactcgcc aagttcggtc tgtaaacgac
    48121 ctcactcttc ctttcttttc ttagaaagct gaaacacaaa aaagtcttcc ggcaaagcgt
    48181 gatagcctgg acacgatggc tacctttatc ctgtcgtatc cagacaaggc ctttgtgccg
    48241 cacggcattt ctgaccaagc tgcaagcgct ccgtccggcg cagcgttcgc gcagtggctt
    48301 cggctctgtg atgaaatcca ccgtcatcag ggacgcatcc tggtcattga tccagaagcg
    48361 gtcggcccgg tttcgccggt ctacgccgcg ctgcttggct caccgtttct gtcgccgggc
    48421 aagctgccac agccggtgtt tctgcgcgca aaccatggcg aaccgcaagc ggaagacgcc
    48481 gtacagcgcg cgatttccga cgccggtctg caagttcgcg ccgcccagca ccgctggcaa
    48541 ggtcaagtcg acctgatccc cgtcggacgc aaccgcttta tcttgacgta tggcagtggc
    48601 gaccacggat cgtctttgca cgccatcgaa gagatcaaag aattgctgcc gcttggggcg
    48661 caggtgatgg cgatcgagct atcggagggc tgcccgcgtg gcagtgccgc cctgacctgc
    48721 atcaccgatc ccagcaacaa gtcggtggtg ctggtgtccc ggcgcgcgct caagtcgcac
    48781 acccccgagt ccatcggtcc gttcgtcggc aacaacgtcg aagtatccat tctgtcgaat
    48841 gaagacgcct cggtgttcgc cagcgagtgc atcaacgtcc gcggcacgct gattctgccc
    48901 gtcggatgct cgacgattct gcgcggcttt ctgatccgtc gtggcttcca actggccgaa
    48961 gtcgacgtct cgcttctcgt cggtccgcaa ggtggtggcc cccggctttt ggcctgtgag
    49021 ctgcccggat tggtgctgtc cgacgaagca ccgtcgtatc ttttgcgccg cgaacggctg
    49081 cacatcctgg ccagcgagta tcccacctag cttccgtcga cccgtcacgc agggagagcc
    49141 cccatgagac tgctcggtcg ctccttcgtc ctcggactta tcagcttgct ggctgtgccg
    49201 tcagcccaag ccattgatca tcaagacgcg ccgtcgctgg cagcaacccc gagcgctgac
    49261 attgccgatc tgttcgcctg gatgtcgaca gacggcagca aggtcatttt ggcgatgacc
    49321 gtaaatcccg gcgccacgtc gagcagcagg tttgcttccg acacgatgta cgtctttcac
    49381 accagcagcc gcgcttcgat tgcatccacc actgcgacgc tggttgatat cgtctgcggc
    49441 ttcgacgatg cacaaaagat atcctgcgcc gtcggcgatg cttcggtttc gataagcggt
    49501 gacgcgagca atccgtcggg tcttgcgcga tccgacaaca agctgcgggt gttcgccggt
    49561 ctgcgcaaag atccaagctt tgccagcatc gccaacatcg accagatgaa agccgccgcg
    49621 aaaacagcat tggcgacggc agcagcccca gacgccgcgg gctgcaaagc gacgactggc
    49681 aagaccgcaa tcatggcgct gggatcgatg ccagcatcga acaccttcac cacaagcaac
    49741 gtgctgtccc tggtgatgga agtcgaccga agcctgctca accgcggcgg accgatattc
    49801 ggcgtatggg ctgcgacgca caaaaaggcc aaaaccaact aacgggtgac gcatgaaaag
    49861 atcatttttt ccgtcgctgt ttgtgctttt tgtcggttgc gctgccgaag ttccagctgt
    49921 cccaccgtgt agcaccaccg tctgcccaag cggtcaagtc tgcaatccgt cgacgggagc
    49981 atgcgaagtt cagccgtcgc gctgcgcgtc cgtaagctgc gtaagcccgc tgacctgcga
    50041 cggcaccact ggtttttgcg tatttcctct cggtgacttg attgatcgcg ccggtcgacc
    50101 gctcatcaac ctggcagtga ccaatccatt tgatctgctg acggtaaacg gccagctcga
    50161 agcaagcgcg acgacacgcg agcgctacaa caaagacaag tcgttcccgt ggtcggcttg
    50221 ggcgacgacg ctggccccga cgctggcgct ttacgacggg ctcgacggaa cctgtgggca
    50281 caccctggcc ctaccggcgc tcggcgcgtc acgctttgcc agcatggcgt cgctgctcgc
    50341 tgctgacgcc atcatcgtcg acaccagcaa gtcgacctgc gcaggctatc tcaccgtcga
    50401 aagcaaggcg ctcgccgcca tgcccagttc cgattgtggg ggtcgcaagc tcgattatga
    50461 cgtcgtcgat gctacgtttt cgctgctcac cggtgggccg gcgaccgacg gcgttccgat
    50521 gagcaattcg ccgctttcga cgttcccata tctgccctaa acaaccgtct tcgcagggtg
    50581 cgcagagcca gcatcggcat tagcatgcag gcatgaacgc agcgcagccc accgcacccg
    50641 acgccgacat cgcaatcgat ccgatctgca agatgaaggt ggaccgtcgc gcacccaaag
    50701 gcggcagcca cgattttttg ggtacgacgt actttttttg caatccgcgc tgccgtgaaa
    50761 agttttccgc cgaccccgag cgctatctct acgcgacgaa gccgcagccg aacaaagccg
    50821 cggaaggcac gctgtggatc tgcccgatgt gcccggaggt gcgagaaacc aagccggtgc
    50881 cgtgtccgtc gtgcggaatg gcgctcgaac cggatcaaat cgacctgagc gcaccgcccg
    50941 atgcaaaaaa ccacgagctt ttgcagatga cgcgccgact gtgggcatct ttggcactct
    51001 cactgccggt gtttgggctc gaaatgggaa gcatgcttct cattggccat catgtgccgc
    51061 tgtggattgt ggtggtcgaa gcgctgctca cgacgctggt tttggttgcg ggaatcgagc
    51121 tgtggcgacg cggctatgtg tcgtttcggt cgcgcaagct caacatgttt tcgcttatca
    51181 cgctgggcgt agcgacggcg tatggctaca gcatggtgcg acttgggcaa tatttgctcc
    51241 tcggtcgcag cggccatcca cccgccgtct atttcgagtc tgcgtcggtg atcacgtcgc
    51301 tggtgctgct tggtcaggtg ctggagctgc gtgcccggca caagaccggc gaagccctgc
    51361 gtgcactttt gacgctgatt ccaccgctag cgcgtcgggt tttcccgtcg ggagatgagc
    51421 gcgatgtgcc cgtaggcgag ctgttccccg gtcacagggt gcggattttg cccggtcagc
    51481 gggttgctgc cgatggtctg attctcgaag gacagagcac ggtcgatgaa tcactgctca
    51541 ccggggaatc gctgcccgtc gccaaatcac ccggcgatcg cgtcgtcggt ggggccatca
    51601 atggcaccgg cgcgctgctg attcgactga ccgaagtcgg agcagatacg ctcgtcggac
    51661 agatcgtgca agcggtgcaa aaagcgcagc gcagccgagc cccgatcgaa cgaatggtcg
    51721 atcgcgcatc ggcgctgttt gtcccgacgg tgtttgtggt cgcgctgctg tcgtttttcg
    51781 cgtggctgtg gctcggcgga atcgggaagt tgcccctggc agtcgtttcg gcggtgtcgg
    51841 tgctgatcgt cgcgtgtccg tgtgcgctcg ggctggcgac gccgatgtcg attttggtcg
    51901 gctcggggcg gggcgcgcag gaaggcgtgt tgtttcgcga cgccgaagcg ctggaacggc
    51961 tggcgctggt cacgaaagtg gtgctcgaca agacaggcac gctgaccgag ggaaaaccga
    52021 cgctggcggc gcttttgcca cgcgatgatg tcagcgaaga tcgacttctt tccgtcgctg
    52081 cacagcttga acaaggcagt gaacacccgc tcagcatcgc cattgtcagc gccgccaggg
    52141 atcgaaacct gccgatgccg acggcaaaac aagtggtcgc cctgcccggt cgtggcctgc
    52201 gcggtgacag cgaagaaggc ctgattgtcg tcggaacact gcatctgctc gatgaagaag
    52261 gcatcgctgt gccgccagat gccgtcggac aggtgtcttt gcttcagcaa gaaggtcaga
    52321 cggtggtgca ggtcgcgctg gccgggaagt ggctgggtgc catcgcggtg catgatcggc
    52381 agcgagaggg cgtcgacgaa acggtcgcca agctgcgcaa gctgggcatc ggcctggttt
    52441 tggcgacggg ggacaacgag aaaacagcgc aagcgattgc ccacaaactg gggatcggtg
    52501 agctgcatgc gtcgctgacg ccacacgaca aagccgcgct ggtccaaaag ctccacgcgg
    52561 acggtcactg cgtcgcgatg gtcggtgatg gaatcaatga cgccgaggca ctcgcgagcg
    52621 ctgacgtcgg tgtggcgatg ggcaccggca ccgatgtcgc aatcaaaagt gccggcgtga
    52681 cgctgctgca tggtgagctg cgctcgctcc tcaaagcgat cgcgctgtcg aagctgatcc
    52741 gtcgcaacat ccgtcaaaac ctcggctttg cattcggcta caacttgctg gccgtaccaa
    52801 ttgccgccgg ggtgctgtat ccgctgattg gtgcgctgct cagtccgatg atcgcgagcg
    52861 ccgcgatgag ccttagctct gtgtccgtaa ttagcaatgc gcttcgaatc agaaatgcga
    52921 ctttagatac ataaatttgt tttacaatcg ggccggggag gcatccgtga acccagcccg
    52981 tttcctacca ctcgccgttc tgttcgtcgt caccagttgc tatcccgaat cggacttgca
    53041 gcccgagtcg cttcgcaccc ccgccgactg cggcgcccgc gaacgcatcg atgcccaagt
    53101 gaaaaaccag gcgctcctcg acttcgccag catcggcaag cagtgcgagc cattcgccgt
    53161 tcaaaccttt gtgcaaaaca agcaagagac ggtgtggaaa gcctgctgtt acgacaaggc
    53221 cttcaactac gcctactacc cagcgaagtg cgtcgtccgc aaagtgtccg gcccggtcga
    53281 ctgcacccca aactagctcc cgtctcgatc ggacgctctc cgccctctgc gcggtttttt
    53341 gagccgcttc cttaggcccc ggccacttcc tactacaaag tcagcgttgc ggcgtagcat
    53401 ctgccttggc gcaagccgtt tgtacaaccc ttctttcgga gaaaccgcat gcagaagaaa
    53461 gaaacgaagc ttggtcgatg tcgttcgtct cgtcggcagc cgtggctgtt ttcggtgctc
    53521 ggttccgcag cgctgcttgg cgcttgtgac ggggccccta ccgaagtcga gcaggccgca
    53581 gtcgaagtga ccaccgaagg cgagtctttg gctcctgacg tgacgccgcg ctgcatcacg
    53641 gtcaagcaag gcgacccgac gagctgcaag gatgaaaaga cctggcgcag ctacggcgag
    53701 atgacctgca agtcgaagag cctctcgctc aagagtgtca ccttcgcgac gtcctgtgga
    53761 atggcgagct ggcgcgaagc cacctacacc tgctgcgatt ccgtcacgcc gcctccgccc
    53821 ccgccgccgc ctcctcctcc tccaggcatg tgcatcaccc aagagctcag caccgccgcg
    53881 ggcgcatgca ttgacgatgg aacctggaag ctcaaggccg aggagttctg caagtcgaag
    53941 ggcctttcgg tcaaaaacct gggctttggg acggcctgcc gaagcggtca tatcaacgtc
    54001 aaggtggagt gctgcggccc gacaccgccg ccacctcctc ctccgggcat gtgcttctcg
    54061 gaagcgctca aagaccccat gggggcttgt gtcgatgacg gctcttggaa gtctcgcgct
    54121 gaagccgtct gcaaggcaaa aggcgcctcc gtcaagtctt tggcctttgg tactgcctgc
    54181 cgcaccggcc atttcgacgt caaagtcgag tgctgtggtg gcatcacgcc gccgcccccg
    54241 ccgcctcctc cgcccccgcc gccggtgcgc tgtgcgtggc tgccggttcc gggaacgggc
    54301 gtttgtcgac tgccggatgt gtggaagcgg gaagcaacca cgctgtgctc gacgaaaaag
    54361 cagacgctca aggatctgaa actcgacaaa gcatgcgcga tgggtggatt caccgacgcg
    54421 caggccgagt gctgcgatgc cacgatgccg ggaccgacgc cgccgccctc gacgtgcgtt
    54481 ggcgcggtgc tgggtgatgg catgacttgc tcgacggaag caaacctcaa ggcggaagcc
    54541 gacaagacct gctcggcgcg cggcatgaag ctcgatggtt ttgcggccta cgatccgtgc
    54601 ggcgccgtg
//
LOCUS       DAORGN010000009        53387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_203_length_53387_cov_5.581471, whole genome shotgun sequence.
ACCESSION   DAORGN010000009 DAORGN010000000
VERSION     DAORGN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 53387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53387
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_53387_cov_5.581471"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKL17_02015"
     CDS             <1..699
                     /locus_tag="PKL17_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43530.1"
                     /translation="EEGALAWHGVRESVRPSEPFAIMEVGGATVQLALADAHSDDAQV
                     VAVSDVRGQDVTFERFTKGGPRPEFAVCYSPSQRRLQRGRACIDFLYDQVFADARVTG
                     LSAVTSPRQVFALGAPWLGLLRELPSSPPFAIKANRDLPQKVRLADLQTLADWICPLS
                     DEQLLAIAPHSYEAKRGAGRTCYSVAYHAAYFQAISHVARAETVIPGGDDQWARGAAT
                     SGLFFPDCAKPSAK"
     gene            711..1781
                     /locus_tag="PKL17_02020"
     CDS             711..1781
                     /locus_tag="PKL17_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43531.1"
                     /translation="MQSLSQMGRPSRLGARRWHFGLLVVCGCGTLGDGVPPPDEWPRT
                     PLRGFVLRNLESPLLIEKAGFDLDAPTLEPDPSMPSLRVFGQLWPRNQPTKSQLFAVT
                     LKNLDDQKSPDELFAATQPWEGQGLSNPVWLPGGLGVDFPSPLLFYQGSDGSVGVMWR
                     QPAGDLVRLSQPLVVASDLTPGKLGRVSPVLFSGRLRLYFTVDDSSLRYAEADASSVA
                     LFLHRPSASVRFVSGPALLTAADFTVAVSRSQQVPAERLGAVFVRSVLTPAGRPRYDL
                     YGRAEAMGKTALVAASSYRGDASEPFLPIADPLLAVTAGGVPGSPTVSESGGISILWL
                     GLRTVASGIAVAVQQKDLPPKS"
     gene            1778..2866
                     /locus_tag="PKL17_02025"
     CDS             1778..2866
                     /locus_tag="PKL17_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNK43532.1"
                     /translation="MSLLFADIEALLGGAVGSAFPSASLSFGQSSQPNTIAVGGASLE
                     TWFDIASLTKALSTTLLAMRLVQKQQLDLHQEVLPGVRLDHLLSHSAGFPACFPPLWA
                     QTSGLLDSPSEATRQAVLDAVLATPRDRAGQRSLYSDLGFILLGSLIEQRGEARLDDL
                     LADWLGPLALALSYRPIDKRSPIAAALVAPTRRETPQREPLRGVVHDDTARAMLGVAG
                     HAGLFAQSASVYRLGQALLDCYHDEDTADARVLGLRPDIVRRFFALPALPGLPGTWGL
                     GFDHPDPLDPNKTSSSSAGTLWSRQGVGHLGFTGCSIWIDPQIRLVAVLLSNRVFVAT
                     TAEADASKSALRALRPAVHDAIARLHCR"
     gene            3049..3957
                     /locus_tag="PKL17_02030"
     CDS             3049..3957
                     /locus_tag="PKL17_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNK43533.1"
                     /translation="MFGAASPATQSDPVEEALLGLLPPKDESPETLHRAMHHAVFSGG
                     KRIRPRLLLTVASACAADDAEMDLAMRAGCAIEFIHSASLVHDDLPCFDDADFRRGKP
                     TVHKLFGEPMAVLVGDALLTRAFEILADTPSRLARRALRIVGLLGQATGSQHGIIGGQ
                     SLEQKGLDGSIIDRYHAMKTAALFRLAASAGAVAAGATDAEAWGEVGQCLGLAYQLAD
                     DLCDAVSSCEIAGKPVRRDETLGRPNAALLTGESVTKQRLVSLILRAQSRTRELAAEP
                     KPLLELIDDLSGYFLKTTQKATPQNG"
     gene            3968..5251
                     /locus_tag="PKL17_02035"
     CDS             3968..5251
                     /locus_tag="PKL17_02035"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNK43534.1"
                     /translation="MKDERSLPTGRLGRLGRLVSAGARAGIDLLGKRDSTHSAAAAAE
                     VLGTLRGLAAKVGQMASYVDGVVPEQHRAAYETAMGALRQAAPRSPYPDIRSLIESEL
                     GGSIGQLFHTFDELPVASASIGQVHRAVVADAAGNPAHEVAVKVQHPGIVAALESDLA
                     NASILEQMMAFSGTRRFNSQGMLEMIRTRFREELDYRLEAQRQKQFAQVHAGDQTIRI
                     PAVHDALSSGRVLTSEFVRGAPFEQAISASEADRRAWAETMWRFVFKGNLVGGMFNAD
                     PHPGNYLFADGGVVTFLDYGCVQAIPPDQHEWAVAVHKTAIARDEQGFAEAGKKLLGT
                     KPGPLEAIVLRYLRRCFEPLFVPRFRMTRQYAASLVDDFKDMTQQALKMEAVYTFAMP
                     TEVLFMNRLQFGFYSVLARLDVDVDYASVERAFLK"
     gene            complement(5375..5572)
                     /gene="rpmI"
                     /locus_tag="PKL17_02040"
     CDS             complement(5375..5572)
                     /gene="rpmI"
                     /locus_tag="PKL17_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNK43535.1"
                     /translation="MPKMKTHSAAKKRFKPTAKGHFKHKQAHKSHLLTHEASKRKRRL
                     RGTKVVHSTNEKAIRELLPYA"
     gene            complement(5658..5729)
                     /locus_tag="PKL17_02045"
     tRNA            complement(5658..5729)
                     /locus_tag="PKL17_02045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5695..5697),aa:Val,seq:gac)
     gene            5837..>6138
                     /locus_tag="PKL17_02050"
     CDS             5837..>6138
                     /locus_tag="PKL17_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43536.1"
                     /translation="MAAEVSQMLVGINYAWRNYAWDFGEPPASDGGGAWGARAAWTPT
                     LSEELADFSVLGLNVVRWFLLGDGTTFGVGAHKPALDPARPGQWRFHDIPELSPAWL"
     assembly_gap    6139..6170
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6171..6880
                     /locus_tag="PKL17_02055"
     CDS             <6171..6880
                     /locus_tag="PKL17_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43537.1"
                     /translation="RLLMVCQDAKIQLLPSIVDFHFCFPAQPVPSAPLVKCGRSDAII
                     DPRKRRLLLDRVVDPIIAVCRRFPDTVYALELINEPEWCTDDGAFFHEKKVVPLAAMQ
                     DFIAEGAQRINRAGLRSTVGFAKHRSMKAWESPGLGLTLHQWHYYLDPEDVPQNDMAD
                     FGPCIVGEFPSARWKPWRELGDAQDTRSRLAHLERRGYDGALLWSANREEEKIAEPVV
                     DWSERVRDEVAAYTAGR"
     gene            complement(6763..12201)
                     /gene="uvrA"
                     /locus_tag="PKL17_02060"
     CDS             complement(6763..12201)
                     /gene="uvrA"
                     /locus_tag="PKL17_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNK43538.1"
                     /translation="MSSMLPISLRGAKTHNLQSVDLQLSAGTLVALCGPSGAGKSSLA
                     LDTLYAEGQRRFVESFSPYARQFLERLPRPDVDSLEPVAAAVAVDRRAPVKSSRSTLA
                     TLTDVEPYLSALFAAEAVPCCPTCQLAATVISPVEAAARVSESHSGRSALIVFPIGTS
                     EEARLALPRLREQGFRRLLLDEELFDVEADTLPDAAQVHVVVDRLVLSEQERSRLCDA
                     LELCHRYSTAAEVRLIERGGQAKDAGARSRYTQAEKILVGKDLHCPGCARTFEPPHAR
                     LFSYNSPLGACPACRGFGRMIGIDWDKVIPDGSKSILKGAIKPWSGQSSQWERAALVG
                     FAEKHRVPLDKPWDKLTPHQQKLILDGEGTWDDGKYPGVRAWFQFLESRTYKMHVRVL
                     LSRYRAYELCAVCHGARLNPTALSYRVGQKNLAEWHADTVATSLERLSGLSPRSSQGK
                     LVIESLHSRLGFLSQVGLGYLQLDRQARTLSGGEAQRASLTTALGAGLTGTLFVLDEP
                     SVGLHPTDVPKLGDAMRSLARGGNTVLLIEHEPSLIAACDRAIELGPKAGRHGGRVLF
                     DGPPKELASRPDLPTGAAWQKHKQKPRQVRPIDKVLSLRGVSENNLCDVDVDIPLGML
                     VAVTGPSGSGKSTLVEDVLYRAVARQLGDSSVDRPGAHKQVSGTQALKNVVLVDQSPL
                     GRTTRGNAATYTKAWDRFRTRLAAQASALSAGLTASHFSFNVAKGRCETCSGEGYETV
                     EMQFLADVSLLCPVCQGKRFRPEVLTVKLLGLHVAEILELSIEEALLRFDPKAEPDPA
                     LRRALAPLLRVGLGYLTLGQPLSTLSGGEAQRLKLARALSEPARNTLFVIDEPSAGLH
                     PLDVEPVVAALHALCENGGSVVVVEHDLDVIAQADWVIDLGPGGGPDGGRIVAEATPA
                     ELARLSTRTGTALKARQLSTLHFADEQATAQTQEAVISVEHAREHNLRGISCKLPHGG
                     LSVVTGPSGSGKSSLCFDVIYAEGQRRFMETLSPYARQFLPQLPRPDVDSVAGVPPAI
                     ALSQRVSRAGANSTVATVTEVAHYLRLLYAKVGVPYCPNCDEPAQATSTEALMTLFAH
                     LGEVPHTLYAAAVRGRKGTYLEVFQAASKAGLKTARVDGKLVAIDPPPRLLKTAEHHI
                     DLIVAQGRPSLFTRAEIDLAIQLGDGNITLAQGITGSSKSSKTASDERSFSVRRACAK
                     CGLGIPELDPRWFSFNTKQGQCEACEGRGHVPDDESKRCRSCQGTRLAPLPRKVRLQG
                     HTYAELTAHSVQQALSQVETWRFQGGEEKLSAAPLHELLRRLRFIDQVGLGYLSLDRA
                     AATLSGGEMQRLRLSAQLGAGLTGALYVLDEPTIGLHPRDTFRLLRNLRSLADLGSTV
                     LVIEHDEATIRAADYLLDLGPSGGKHGGSIIAQGPAAVVLKHPDSPTAHALAEPMPTR
                     TPRPLGDKQLSLRGASVHNLKNVTLKLPIGRFNVVAGVSGSGKSTLVQKVLYPTLRKA
                     LGLSTEAPLGHAKLDGIDSVRRALLVDQAPIGRTPRSVPATFLSVWDEIRRLYASLPD
                     AKVRGFAAGRFSFNSAGGGRCPTCEGQGVQVSEMSFLPDVITPCEACAGQRFEPATLA
                     VRFQGLTIGDVLMLSAEEAVRHFANHPKIMRPLSTLCELGIGYIQLGQGSHTLSGGEA
                     QRLKLAAELTALQKHEPTVYVLDEPTTGLHLSDARRLIALLHKLVDRGDTLVIVEHHL
                     EMIAAADWVVELGPEAGESGGEIVFAGPPTALAQANTATGESLAQAWAQRPAVYAATS
                     SRTRSDQSTTGSAIFSSSRLADHSSAPS"
     gene            complement(12377..13240)
                     /locus_tag="PKL17_02065"
     CDS             complement(12377..13240)
                     /locus_tag="PKL17_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HNK43539.1"
                     /translation="MRSNSPRGLLLLAVPLLLLGCIGPGMLSDGSSVAMGNFNNGSLR
                     RGRKLPEEGPGYTIPTLWRERGANYGTDEIVNAIMRAARRVAKEHPGGTLGVADIAMK
                     GGGESKLHRSHENGHDADLIYYAIDDDGAPVMPVSAMPRYGAGLRSLNVRPTPGVQFE
                     AFTARKFDVRRNWALVRALLEDPEIEVQFLFIHNRLRNVLLKHARDLRENEELIEHAE
                     AILKQPGDTLPHDDHLHLRIYCSPTDRSLGCKDGGPLRWWRKRYKYMPPRPKHDSPPL
                     LSKLMTTRTLR"
     gene            complement(13322..15100)
                     /locus_tag="PKL17_02070"
     CDS             complement(13322..15100)
                     /locus_tag="PKL17_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43540.1"
                     /translation="MRGKLLFATLLLCAPSWVEARPLYRGKVSITGFYYTEDDGVDPT
                     QNIDKRIATNSNLGLIDLRATVLASRLWNERLDFKLDFRLRVTGSLDFERKYDPNTTL
                     FGDQFYKTPLGTSARGYLGGREYDLREAYATFRPVSTWHIQVGRMYIPETDAIKIDGA
                     KVAHDFGSHWYGSAFFGGYPNPYSRSLLTDYVAPCGNGVAGTRNDTPTLIAAALTPTA
                     DELKTQQCQQDGGRFGLVGGAGARYNYDVLWGSVGAAASFFFGPGDGGPVMPNLSASS
                     PTDGAPIQQNLVAPSDSPDAPRVFLSWFNSWRPHERVDLMSDLVVDLYGSAGPQLTRA
                     VVMSSIRALSDDRLTVRLGYSHMSSLAINMYLARLVYNRSAGTTLAAIGASSVENNLT
                     VLRTGRDEGRVTLDSRFFRRLGGFVEGRVRARSLINGESVPDVYSQKNYTKNTSNLAG
                     DVSLGLRDAGTLFGVRASLIYSLIFDYRAQNHVVSFDVGRDFWKDRITASVGYTAAIT
                     KDQLAGNDAAFCTSADPFSGCFGKRSGITHEANVQVSANPWRTLFFLLDYRLIAMSSD
                     DRKDLPTKEVPTVITHAILFRSEFRW"
     gene            complement(15097..17499)
                     /locus_tag="PKL17_02075"
     CDS             complement(15097..17499)
                     /locus_tag="PKL17_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNK43541.1"
                     /translation="MSCLFHRLPRALFALLLLCGLLACPTARASFFDSSPGKLARGHA
                     EIDGPANCIKCHPQGKREVDEKKCLDCHKPIAERIAQKIGLHASPKALGRPCVLCHKE
                     HRGVEADLYGWVSIGGQNKFNHDTTTRFALQGRHSVVDCKDCHKQKTDSGRPSFLLAP
                     MACVGCHKSPHGELHEQVANCERCHDAKNWRQLDPMKFDHNTDTRFPIETKHAGVPCN
                     SCHPRTTFRLTNWATDCTPCHKNVHGESLFGQKKCTQCHSAKVEWKVIDYDHNHKTRF
                     ALEGPHKKPCASCHPSTATKSPGRNCDACHKDAHEGRFTKTGDCTTCHLSTTWGPELK
                     FDHTKTRFALLGRHAAVSCRACHRGKTPTEFENFDALVKTTTTGKGKKNTTTVLDCMG
                     CHQHTQVHKKQFSSDQCLQCHQKPGEVKQSVDPKAINERVKIGHGPGKPFQLIDGHVI
                     DGIRIKDCRTCHKNDQYRDTSSMCGSCHEDRLHKGSLGKDTCNNCHEGARWGATRFDH
                     DKSNYPLVGKHKDARCEGCHPNRRYKPTPMKCADSGCHLKDDAHQRSLGIKCEDCHSP
                     TGQISFDHNDPKVKDRWKLDGKHNAVRCQGCHPSQKYKPTPTQCQDCHADPEAHKTEL
                     GIRCQGCHEPATWKKIHTGHDIFPVRFGGAHDRLFCKECHPSGRVLQGLAQLCIGCHQ
                     RDDVHHNSLGPRCSDCHTQQTFTGARFYHDRVGCTLTGVHRVLPCSDCHKGGNYAGLS
                     PACVSCHRDDAVRAAALRITTDGLPPGAANRHDGQVSCGSCHNTASFRVSRLVGTDSV
                     CR"
     gene            complement(17583..18371)
                     /locus_tag="PKL17_02080"
     CDS             complement(17583..18371)
                     /locus_tag="PKL17_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNK43542.1"
                     /translation="MPSRLRKERQGDIVLLTLCHLQRANAVDDRLLVALREAIVEAAE
                     QGARAAVLTGAGGVFCAGYDLHALPANPDPEWLRGHGELEATLRLLREGPLPTVAALT
                     GPAVGAGLEIALSCDLRVAHSEVVLQMPPVKLGIVYTIEGIWRLATLCGAGKARELLL
                     LAEPVSAEAALRLGLISRLVDSSVVLETACALAQKMAAKPRPAVQGTRHLLEHLLRSG
                     PSLSEEESERLLEIRQQSWQSPEARSARESLRARRSKPPAPPKQ"
     gene            complement(18422..20239)
                     /locus_tag="PKL17_02085"
     CDS             complement(18422..20239)
                     /locus_tag="PKL17_02085"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HNK43543.1"
                     /translation="MSRTKAPLFCITPCQSLDVTPHSQRRSRRLSHSLVLAATLLGFT
                     AQPHAAPKSPATAAAQTTDESARLSAYVDGFVAELGRHYPTETTELGLHDHDGDLEDY
                     SPQGVLSTLAWLSDWDRKLATLHKDQLTPDEQFDLDLLRHSIATRRFELSELPAHRRK
                     PMAYIGLVSHSINALLKRNYAPLAVRMRPLISRLRKASALIRLAPKQLDSMSLASIDV
                     TLRTLSATTDFFRHDVVQAFSEVPDPGLQAQLRSASADLVDALTALGTFLEKDGKARA
                     TADFALGEAMFQKALWAEEMIDAKVSDLLPMAEAELARLRTEFDKTAAQIDPSRSAAD
                     VQQRVSADHPQPSELIDYTASRQAAQHKYLIDHNIVTVPKDVLPLVRETPPFMRATTL
                     ASMDTPGPYEKASEAYYYITLPEPGWKPEQTEDFLRGAYSRPLIDVVTVHEAFPGHYV
                     QFLWLGKLSRAKKLAHTMSNVEGWAHYTEEMMLEEGYGKGDPKIRLMQLQDALLRAAR
                     FVAGIRMHCRGMTQAQAADFFFHEGRQMRAVAEVEARRGTQDPLYIVYTYGKLQILKL
                     RQDYKTKQGANYSLRKFHDTLLGYGPAPLAMIRQAMLGK"
     gene            20377..20940
                     /locus_tag="PKL17_02090"
     CDS             20377..20940
                     /locus_tag="PKL17_02090"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HNK43544.1"
                     /translation="MTNRNSRSVSFFRSARSVLSALAFVALLPSAAWAEPKAGDKLQN
                     PEVRDARDQPAKLPEWGNKVLLILYTDPDEADQNDEFADMVKASGLSKESFQSMGVAN
                     MKDAPAKPNWIIRRVVRKKIEKYGTTILTDPDHLLIKAWNLGDCNDKSVVMMVDKEGT
                     LRYFFKGKLPEPERKKALDLLKSLIGG"
     gene            21004..22566
                     /locus_tag="PKL17_02095"
     CDS             21004..22566
                     /locus_tag="PKL17_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43545.1"
                     /translation="MSSSQHSVIALAGPRGRPRLSLLLLAIALCSALWLVGAGRRIDY
                     LADDSYILFRHVLNHSRGLGMIWDLWHPVQAHSSILWAYLLRLAMVLGIPIEDGARIL
                     GVSFGSGAVLVLTVTLWSKLNLRRLLLAVLLLATFPPLVLWSSAGLETTLALCLGLHF
                     LSVVGRACESRVHPRIRDAELITAVLLGVVSPLVRPDLPLQLLGGLVCLFIWHPRSRS
                     LIPMVGLSCLLGMVVYVALNRSSYGAAVAVPVATKFRIEPRNILRFLRYVQRDAPQLV
                     ILGIGLLAVLRKTWQSELCQDPISLLSLGALGATLIELSLLGGDELSRGRFLIWPYAC
                     TLLCLGRLSMAVRIPKGIRSGVMLSLLLLSFAQSAWALEPEGDQPCSVWRRAAGKWIH
                     SHSDPALTIAVAPAGFIPFFADRNTIDLMGLAHPQIGRLKLPRGSVEWGRLATEIAIA
                     EGVCAVLLVVCDSPSEDPRQQCAHPTLQGMVDALVRHPEFRFTEVPIGVGNPLRMAVR
                     SDCLKHLRGMDR"
     gene            22631..23221
                     /locus_tag="PKL17_02100"
     CDS             22631..23221
                     /locus_tag="PKL17_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNK43546.1"
                     /translation="MSGKQIDFYFDLSSPYSYFAATQLDAIAGRFGAQVVWKPMVLAA
                     VFKAVGNVMPAVCIPKAQYMVKDLARWAAHYGVPFQMSSRFPLPTIRAMRLVIAGEAL
                     GKSGDLAKRFFSAMWERDLDISSDDVLRQIIGEAGLSADALFAATDTAQVKDKLRAYT
                     DEAVSRGVFGAPAMVVDGELFWGNDRLHFLEQKLAK"
     gene            complement(23269..24057)
                     /locus_tag="PKL17_02105"
     CDS             complement(23269..24057)
                     /locus_tag="PKL17_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43547.1"
                     /translation="MKSTLSLVALLGFSLAGPSVRAQVPSIQPQGQPDGSMGPAATAS
                     AKDQAPIPPGQVQPDASGQQLAEPNLNPLPPQAAQPPIGTMRHAPRVGAIALGWMYSP
                     ISYLPLSADNVGGKFQTQGLRVDGKFGWQVGGFTSRYASYVGFMAGFYYNFGGMVADS
                     LGIDYGVFAKHMISPGPRVRGFLSYGLGATQVWVRQLGGRGIGHVTRLSGGVDIKMTE
                     RIQFELELAYQFNILATFAGPQAEPSRSYDFHALNLTAGLWFGK"
     gene            24089..24991
                     /locus_tag="PKL17_02110"
     CDS             24089..24991
                     /locus_tag="PKL17_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK43548.1"
                     /translation="MSTNPSPPRRVPRKTPVFPKDLLDGQVVIVTGGGSGLGLSMSAG
                     LCQAGAHVVICGRNPERLGQAVETLRTGGGKADAIPCDVREPEQVAELVKKTVERCGK
                     IDGLINNAAGNFLCAAEELSVGGFDAVVKIVLYGSVNATLAVGKHLIERGAPGSVLSI
                     LTAYAWTGSAFVLPSACAKAGVLAMTRSLAVEWGIYGIRLNAIAPGPFPTEGAWSRLV
                     PEASMIEGLVEKRVPMGRVGEHSELANLAVFLMSDASPYQNGDCVTIDGGSWLAGAGE
                     FSEYARYDRDRLKTLLKAMRPKKS"
     gene            complement(24993..25712)
                     /gene="uppS"
                     /locus_tag="PKL17_02115"
     CDS             complement(24993..25712)
                     /gene="uppS"
                     /locus_tag="PKL17_02115"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNK43549.1"
                     /translation="MKKRADHVAILMDGCRLWSTERGLSPSSGLWASALTAAGAIEAA
                     AQSGVRHLTLIPTDTFPMDRSDEASTVLFQFLRSHGETFRHLSLRFSAHGELSCLPAQ
                     LQNKLHAMQTASSHELGMGLSLLLGYSGRRDLLRVAKELAAEVKAGRLPAEAISPALI
                     QQRLATASLPPVDLIVYTGRGQDLADALTFESAYAEFAFAHCLWPDFTAEDLQHALAD
                     FGHRERRFGKTSAQIESPTPS"
     gene            25894..>26541
                     /locus_tag="PKL17_02120"
     CDS             25894..>26541
                     /locus_tag="PKL17_02120"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNK43550.1"
                     /translation="MSSLKVEERFVVDADVLRVWEFLKRPALVAGCLPGAKLDGQEGG
                     DTFLGTMKIKVGPVMQDFRGKATLAEVDDTLHKMTVSGVGDDKAGGGSARMTMKCQVV
                     PSSETGKSEVTIVAEVELFGKLVRFGRGMFEAVAKQLFKQFVDRARSAIASMPAPAGS
                     SEPAVVLATPLPATSTGTSEASTGTSEASTGTSEASKPTSEASKPTSEASKPTSEA"
     assembly_gap    26542..26641
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    26650..26749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26925..27053
                     /locus_tag="PKL17_02125"
     CDS             26925..27053
                     /locus_tag="PKL17_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43551.1"
                     /translation="MATPNQRVVKSNDDEALDAGSLIFAVIWEWIKGLFRRLFGRK"
     gene            complement(27071..29101)
                     /locus_tag="PKL17_02130"
     CDS             complement(27071..29101)
                     /locus_tag="PKL17_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43552.1"
                     /translation="MSGEAGFTSQQQQPSPWALGGGFGGGGHFGELGDAGVGQGFQAG
                     GGHFGELGDASPWLTGGGFGGQWSEHEGGSGQKGEGQGQERTLNPDKQGSPAIKEAVG
                     NAASGKDAKGGKPDQAILTPQQIEQQFKEGKGQDFERAKALGMNAEQYKQEYVHEYMA
                     RLCQTDPFVKDKDGKPVVGKDGNPVSNYSAAQQNSLSAWGYNPKVDPSNVVDHNKDTG
                     LFAIRFEPSAQGAKNGTAPVVAFRGTEPDRGPRDAQADLGDKYIGQSEYEPGKAEIAK
                     LFGHKPGEKADVVGYSLGGALAQRSLVDNAQNVRSLSTFQSPGIAGQDAAAVNAAEKK
                     YGIDVNHHFFTNDIVHRAGEAKVDGNYHEYTRGDWATGLNTGDAHLSSFLIPSDGAKG
                     TIDAHGKVKDKDGYANATNQNTTIQSYQGDRQSDRAFWEAQRRTLGPAASGLLGEVGA
                     GMDLWQKTDSEWNPVLGKDGKPIPGPLQNAVNQTKGGWGETKDGWSGVKSGATEAGHG
                     VSDLLHGNFGKGLSELGHGLSSGWSGAKEMGKGAQDMWSGVKNGASTLGNFFHDHIAP
                     HGGDLKQIASGLPGIVNHLAHDPSVQKKVAEMAAPITKPIGAAIDKAKSIGGAISNGV
                     SSAWDGAKSTASKVGTGISNGVNSAVGTAKNVANNVGSAISNGWNWLTGKKK"
     gene            29264..30571
                     /gene="murA"
                     /locus_tag="PKL17_02135"
     CDS             29264..30571
                     /gene="murA"
                     /locus_tag="PKL17_02135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNK43553.1"
                     /translation="MAATQYIVEGGHRLHGTVRPAGNKNAALPIIAAALLSQDKVTLR
                     NVPRIRDVETLVDLIRSVGAEAEWDVSDIHTLHIHAKTLRAASLDPDLCARIRASILL
                     AAPLLARCGEIHLPPPGGDVIGRRRVDTHFLALGELGATYQLSERFHLTASKLRGADV
                     FLDEPSVTGTENAITAAVAATGTTILRNAACEPHVQDLCNFLVTLGAQIEGIGSNQLT
                     IHGGRALHGGEFSIGPDHIEVGSFIGLAAVTRSELRIAQAGVRHLRMILLGFERLGIT
                     CKVDGDDLIVPSAQSMQIRSDLGGHVPKLEDQPWPAFPADLLSIAIVTATQCEGVILM
                     FEKMFESRLFFVDKLVSMGARIILCDPHRALVYGPSLLRAGTVESPDIRAGMAMLIAA
                     LCAQGKSTILNVGQIERGYERIVSRLRSLGAHIEEVEDRRSRR"
     gene            30664..31353
                     /locus_tag="PKL17_02140"
     CDS             30664..31353
                     /locus_tag="PKL17_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK43554.1"
                     /translation="MKVSIIIPVYNEERTIGAVIDLVSSLTFDKEIIVVNDGSSDGTR
                     EALRPYESRPGLRVYHCPVNLGKGSAVRIGFSMAEGDIVTIQDADLELDPRDYYQLIE
                     PIVSGQADVVYGSRFLSQDGTSHRRGMLMFYLANRALTGFANLLFHGHLTDIETCYKL
                     FRREVLPKLKLRAARFEIEPELTAQVLKNGYRIVELPISYKPRTHAEGKKIAWVDGFI
                     AVYTLAKQRFG"
     gene            31502..32641
                     /locus_tag="PKL17_02145"
     CDS             31502..32641
                     /locus_tag="PKL17_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNK43555.1"
                     /translation="MGCTSSGTSGNTDAAGLTDGGDGDLAMATCSPEGVFDGAPVTAA
                     AGQWAWVDEPKALCRDGSATGFGVRINPNSDKLVIYFQGGGACFNSATCGSNPATFNS
                     GTFALAVLAGGQQGIFNQTDSANPFRDWNAVFIPYCTGDVHAGTATGVTVPGVGAPTN
                     QQFVGYRNVGYYLKRIIPTFPKVTRVLLTGASAGGFGSMLNYHRVAQAFCPRPVSLVN
                     DSGPVFQDAFLAPCLQKRWRQLWNFDANLPAGCTGCSNADGSGMANYEKFIGQNYPAQ
                     RLGLISSLQDATISYFLGFGQNNCSAIDGTPGPLPGATFQAGLIDLRDSLLKPNPNWS
                     TYYLTGTTHTYLNNANYSSLTSAGVKLTDWMIGIANQGPSSHVAP"
     gene            complement(32683..34641)
                     /locus_tag="PKL17_02150"
     CDS             complement(32683..34641)
                     /locus_tag="PKL17_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNK43556.1"
                     /translation="MRRFRTLLFSLLLSTAPDAVGAPPKPAAESNNDKPLIPRELASD
                     EDIARALREHYTKHEFRIPMRDGVRLFTHVYVPKDSSRRYPFLMTRTPYGIAPYGVDN
                     YPSAKEPRQLRGLAPATALIAHGMIFVQQDVRGKLMSEGVFSDVTPYIENKRTAKDVD
                     ESSDAYDTIDWLVKNVPGNNGKVGIWGNSYPGFYAAQAAIDAHPALKAVSPQMPVTDW
                     YMGDDFHHNGALFLADTFLFYANFGKPRPKPSPKARWDFDLESGDLYDYFLTVLPLFD
                     ADTRIVGTALPFWRACVEHPNRDDYWKARDPRPHFRNIRPAVMTVGGLYDAEDLYGTL
                     GTFHAIEKQSPSTQSTLVLGPWKHGGQYRTDGDSLGDIHFGAKTSRYYQEHMLVPFFL
                     HHLKGISAETPPKAWAFETGKNVWRSHESWPPATAKPTPFWFAEGGGLSIDPNSKTGD
                     AFDRYISNPHKPVPYRERLSAHRDPEYMVEDQRFASRRPDVLSYVAPANDADVTVAGP
                     IDVDLWVSTTGTDADFVVKLIDVYPEHAADPEPNPREVRMGGYQALVRGEVMRARFRQ
                     SFERPEPMTPNQPTRIRFQLPDVFHTFRPGHRIMVQVQSSWFPLVDRNPQQFVDIYRA
                     TEKDFLVTTHQVYRGGDKRSSVTLPLLK"
     gene            complement(34776..35342)
                     /locus_tag="PKL17_02155"
     CDS             complement(34776..35342)
                     /locus_tag="PKL17_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNK43557.1"
                     /translation="MKRIAFSRLSVLLITTCSALTEYGCNSGTSGPVDMAAPQAVAQI
                     NEPGGAQGTATFEETSDGVKITVNITGVPTDGNFGMHIHATGDCADTTGDGGVHHGAA
                     GGHFNPDNVNHGCPSATPHHAGDLGNITISGGKGVLQLVSKDLTVSAGARSVVGKAII
                     LHGGTDDCATQPTGNSGSRVGCGVIAAK"
     gene            complement(35365..37011)
                     /locus_tag="PKL17_02160"
     CDS             complement(35365..37011)
                     /locus_tag="PKL17_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNK43558.1"
                     /translation="MPTSPLPSAHESRKLPTYKDTPFAALRFDNRFIRELPHDPDQTN
                     QRRQVHGACYSLVQPTPVSSPRVIAHAKEVAALLGLSADAQEHATFAEIFGGCALAEG
                     MTPYAACYGGHQFGNWAGQLGDGRALGLGEVLTSSGTHWEVQLKGAGPTPYSRGADGR
                     AVMRSSVREFLCSEAMHHLGVPTTRALSLVATGDSVIRDMFYDGHPAPEPGAIVCRVA
                     PTFLRFGNFEIFTARRDVDRLRLLADYTLRHHFPDLGRPHRDSYVRLLGTIAERTARL
                     MVEWQRVGFVHGVMNTDNMSILGLTIDYGPYGWLEDYDPMWTPNTTDAAGRRYAFGNQ
                     PHIAFWNLVRLAEALYPLIEDAAPIKEALLAYPHTFEREQQAMMMRKLGLTTWRPTSD
                     DALEKELYRVFQLVETDMTVFFRKLALLRQDQANLSQFLDETLSDAYYHPQDLSGPHR
                     EAILDFLAIYRHRISDQPLRDEDRRAAMNATNPHVVLRNYLAQLAIDRAEQGDGSLVY
                     ELLDVLRNPYDDNPTWSQYAAKRPEWARHRPGCSSLSCSS"
     gene            37206..37655
                     /locus_tag="PKL17_02165"
     CDS             37206..37655
                     /locus_tag="PKL17_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43559.1"
                     /translation="MRLTSVIMSLFVASCSTTWDAFRIANQDACTNHPLDCGSGKVCS
                     YETRQCQPQNCRQSGFCWESPRPQLFSPRAIYGSSASDLWMLGTREIYRANGSTITPV
                     ITELPVRMNAIVGIAPDNILFLGEQGTILSANYIAPTVSVDDLRMAS"
     gene            complement(37752..39122)
                     /locus_tag="PKL17_02170"
     CDS             complement(37752..39122)
                     /locus_tag="PKL17_02170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNK43560.1"
                     /translation="MKRFALLLLAFWATGFDYFRLNRPVPSSDVVIRMPQLERQILPS
                     GVQLIVHSDAYLPLFHIRVQFLSGLAVEPANQRGALRLLFRTLVSENRALLSALDELG
                     ATPSLAVDEDAVSVAVQVRAEDATSAISAVLRALVEPKWDEAELKNAQQSLYRELATK
                     TQSPHAAARLALNRILFPKDHPLCRNPKDEAAEVKKRNLEELKVLHRSFVVSRNLAIA
                     IAGRIAPPVAAVWVKKSLTGLPEGDVPQPVLPIPKPEKRTASYVVPYPGLVQSVIFIG
                     RRAPSDGHVDAPALEMAAGWVSTNVGQQLREEKTVTYGASAHYQKSAQSGVFVVTAAV
                     EAQHTGYAAREVLSQIFAATSDTVNSKLLPMYRAHMVRDITEESASLAGIGALSEGIF
                     RYGPDHWQKRIAKIQTVSHPEVYKALRDYVVPDTLEVVIAGDPAVIEPQLKKEGLGPV
                     RILGTP"
     gene            complement(39119..40480)
                     /locus_tag="PKL17_02175"
     CDS             complement(39119..40480)
                     /locus_tag="PKL17_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK43561.1"
                     /translation="MPKPSGSLWPHWMILGASVFALPHTASAQSLIPADVLSLENGMT
                     TVLHVDRRQPSVAVMLQVQVGARHEALGRSGLAHLFEHLYFHGSEHLLPYSPRWYLQT
                     AGVRSMNGSTFLDETIYYEVVPSENLEPTLWMESDRLGFPVLRDLDLQDERKIVKNER
                     RQRREINPYGGTDEVSHKRLFPAPHPYFGMVIGEQTDLDKLTLDESKAFFRKYYDPKN
                     VTLILSGDIDKTAAASLVKKYFGTIQSHGKDNTPPDPKPVTVSEEAVLSHDDPLLKEP
                     VLLLRWLTPPTCSAEDRTADVLATILSRGKQGRLHKLSQKWPEQILSDGASQDSHRLQ
                     SVFTVHIAAKDRGTLEGAKELVNKELQELAEKGVTDAELVAAKKKLVNDWLSDLQSPL
                     AKAFHLARAISCHGGPQGVERSLAQVQAVRSEDIQKFVKAYLGQKQCVVTFGMPQSQE
                     SKK"
     gene            40777..42189
                     /locus_tag="PKL17_02180"
     CDS             40777..42189
                     /locus_tag="PKL17_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43562.1"
                     /translation="MKKRQTLIGLFVFCACQASPPVEGGNVGCTDSTTACIAADGCCP
                     AGCTSATDSDCPVPPETALADRLQVVTVTAPAGVIPGVSNYRIWGSTSLVVGPVYTVP
                     FADCGTLVGYTTGTVAAPRARVARLDAQDKLVTTYDLGAFTVRGLAAEPDGHFAALLW
                     DQATNPKSLSVSRYDAQGMAGWTASLADPLAAPTDFGIGESRLEYGAGKYGAYFHVHG
                     ISGFANGHEGDALHWVDAASGMHTVGWNWGCSHSMSELLRYGSAANATLAVCVTDCYP
                     GTTGSTFATDSIGGVYLEKATKVRDFDGGCNGSVAAELGSAAPGLAGWKLLFNGHQSA
                     AKNGQSSYSTATMNQDIGFVSIANNKTAGNIVWLTTTPGNEANSSIARWQPMGNAAEQ
                     YVVGWSSGTTYKLGRVSDTGAFLEGPIDLAAAKWGQRDDPFRTHLNGDIVWAWFDAAG
                     STQLKFARLRSGGTATCTKL"
     gene            42263..42961
                     /locus_tag="PKL17_02185"
     CDS             42263..42961
                     /locus_tag="PKL17_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNK43563.1"
                     /translation="MNDRRLTCIVGGLLLCLVAMYLWGIWSLVSPQLWALRWAHQMTG
                     GWGRAIGMVSVLNFALIEALVFVSDPRKKLRAIEPEIAFIKKNHAADRQKRDILLLEL
                     YRREGIEGFALWVKPLMALVTIWLLLAMYVALWKIPELRGASFFWIPDLSARDPLYLL
                     PVGSVLLSVLGWLAAPNTLPPSESRTRGLVPTLLFACVVFLFPASFVLWGFASKVIVL
                     SGNVGKRILILFRT"
     gene            complement(43001..44236)
                     /locus_tag="PKL17_02190"
     CDS             complement(43001..44236)
                     /locus_tag="PKL17_02190"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNK43564.1"
                     /translation="MTSESRDMDPFPATAPVEASVLGDMTAETFLRDHWQKRPLLIRQ
                     AIPNFRGLFQPHASGKSASTAARDAFLELATRKDVTSRLVVAPQTKGKQPGRWERYDG
                     PFDRLDGRTLPKNNWSLLIHGIESHVPGGWELLRRFSFVPQARIDDLMVSYASPGGTV
                     GPHDDLYDVFLLQGPGRRRWQVSTQADRTLDSEAAIKVLKSFVPEEEWLLEPGDILYL
                     PPGLAHYGVSDEPCFTYSIGFLAPSHRELVQNFLGYLNEVLEEGIDPDALYEDPDLRT
                     QENPLALSDAMVDKVAEVLSQVRVSRDMVGDFLGRLLTGRKLPESFAAPSRTLSLSAF
                     RRRMQGQGHLELAKISRGLVRGDCLFLNGEAHHVLPQTLQTFCQLVQHRSLPLPLLCD
                     GPTVELFHDFYRAGYLRIV"
     gene            44384..46363
                     /locus_tag="PKL17_02195"
     CDS             44384..46363
                     /locus_tag="PKL17_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43565.1"
                     /translation="MRIFLITPPMTQINTPYPATAYLTGCLHRHAPSVEVVQSDFSQE
                     LFLRLFSQRGLQRVFDELSMRARRLRGRSSRIDMPDSIAHFLKHGETCVALIEPVLRF
                     LQGKDPSLALRIVGRTLLPEGPRFANVDHVAGNLDGKAPLDAAFGKLGTTDRARHLAS
                     LFVDDLADVLRDGIDPRFELSRYGERLASSAPSFDALWEALEDEPTLVDETLDELTRE
                     KLRAFPPDLVGITVPFPGNVYGALRIARMVKSIHPKARVVLGGGYVNTELRQLSDPRV
                     FDFCDYITLDDGERPLLALVEHLRDPNQPLVRTFVREGNRVVQKTTPALLDLHHRDTG
                     TPTYAGLDLSQYVSLFEMLNPMHRLWSDGRWNKLTVAKGCYWKKCTFCDLSLDYIARY
                     ETATADVLVDRIEALIKETGQTGFHFVDEAAPPAALRALAERLIARRVAITWWGNIRF
                     EKSFTKELVELLARSGCIAVSGGLEVASDRLLALMQKGVTVAQVARVTRAFSAAGILV
                     HAYLMYGFPTQTEQETIDSLERVRQLFAEGCIQSGFWHRFAATAHSPIGQNPDVYGIR
                     LLRQPRVRFARNEVPFADPTGCDHDKLGIGLRTAIYNFMHGIGLDSDVRIWFDPPSAS
                     RSRWSRRGIRGANTMAVPAPEVPPDLIRQALCASP"
     gene            46471..46950
                     /locus_tag="PKL17_02200"
     CDS             46471..46950
                     /locus_tag="PKL17_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNK43566.1"
                     /translation="MGFVVEPIGVVHCSRTAPEDDRWDSETCLIRLDPSRLSPDAALG
                     LDGFSHVEVIYLFDRVDEAKICRGARHPRGRKDWPLTGILAQRAKDRPNRLGVTVCRL
                     LSVSGFDLSVAGLDAIDGTPVLDVKPYLAEFGPRGELRQPAWSTELMQDYWESKSKP"
     gene            complement(47005..47694)
                     /locus_tag="PKL17_02205"
     CDS             complement(47005..47694)
                     /locus_tag="PKL17_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43567.1"
                     /translation="MDLNFTAVVLSGLAWTITYIALVYRGFRDRSYGMPLVALALNFT
                     WEIVYSQIYPPSSIKLVGTIINTVWMLCDAGIVATFFLYGYKYFERHYGISRAEFYVF
                     TFLAFLASFGVMILGAPFFKDFLPYFGGDPFESGKFIAFIQNAAMSVLFVAMFYQRRS
                     IEGQSFTIAWTKWLGTSMTVGLSYLGIEHRDNWRFVGVFVAVCFVFDLWYMVLIYRQL
                     RREGINPWTRL"
     gene            complement(47713..48456)
                     /locus_tag="PKL17_02210"
     CDS             complement(47713..48456)
                     /locus_tag="PKL17_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNK43568.1"
                     /translation="MQIPSHSESTPTADSITSVYHQISTRRTVRDFSDRPIDPELLRR
                     IAAAAGDAPSSVGIQPYEVHLITNPELRDQLALACNGQRAARSAQALVVFVVGVSIIR
                     RRIEESRTYYQSAPLPEKSRQYHLSGLDRLSLALNPWLLPFLGVARSILSVLQPSRSL
                     LPLGQEGAKNWAARASMLAAQTMLFSASAHGVDACPMEGFNGAQVAQALCLPRGTAPT
                     LVVALGYRAEDARIEPRYRRTADQMITTH"
     gene            48525..49103
                     /locus_tag="PKL17_02215"
     CDS             48525..49103
                     /locus_tag="PKL17_02215"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNK43569.1"
                     /translation="MAKVSTRKTPQQARSKETVAAILQASTYILIEDGWRSFTTNRVA
                     ERAGVNISSLYQYFPNKTAILAALREAHVADSRAALIKAIADTHDPLRNMVHALIDSH
                     RIAPQLHRVLTEELPYSLRRNVECIDHPILHAVVRNMFSAAPDPDLAFFVARSALHAV
                     VHDAACQRPELLSSAAFVHEVERLALSLVHRT"
     gene            complement(49626..51251)
                     /locus_tag="PKL17_02220"
     CDS             complement(49626..51251)
                     /locus_tag="PKL17_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43570.1"
                     /translation="MSWVALVGPELEENLSLRYLASVLTQAGIRAEIIPFNSERAFAS
                     VLSAIVDAATPPLVVGVSLAFQWRAKDALALVVALRHRGYQGHITCGGHFATFACQEL
                     LRDFPEIDSICRQEAEITFLRLTEAVRDGTPLAHVAGLGIHNEDGQVVLTDHPPLPDL
                     AKLPWPDRRGTPARCFAHGIAPIVGSRGCYANCSFCCIAAWHQQSLPGTRYRLRSVED
                     IADEMVAEKRNRGIEIFVFHDDNFFVPGHQRNAERFSALADALEARGIGRFATVVKAR
                     PTDADPQVFEILKTRLHCIRMYVGVESDSEQGLRTLRRWAHSKQNHEALALVEALGIY
                     TCFNLLLFDPDTTLHSLRENVRFMQRASTLPFNFGRVELYAGTPLLSRMQQEGRAVGD
                     YLQWDYPLASAAVERVFTMTMAAFRERNFGADALANRVMGLRFDIEVCRHFHPECFLP
                     SFFQEGLRITRELSEDSVSALSTIIDHVGSNPDESGDRQVVDALIPRLRSTETRLSAD
                     SQRLAKTLQLAVGQGFPLSDVGDVATPLQSANR"
     gene            complement(51254..51796)
                     /locus_tag="PKL17_02225"
     CDS             complement(51254..51796)
                     /locus_tag="PKL17_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43571.1"
                     /translation="MSQAETPKTRMEEVLPTPEKVSPHSDGRGVRTRVLSHMSKLMMG
                     GVGLYTLGCTGFGCVDPLPDPSRCVDEQRKLTATGQYVSDPMGLLLEVKLTPTAGPSS
                     IRFDSSSSSPSGATLVSSQVDPVTGVLTMRLLPTGTTNIGVFLGATCQGASRGWFDLS
                     ITPSMAPKVGDVVVVQVEGN"
     gene            52085..>53387
                     /locus_tag="PKL17_02230"
     CDS             52085..>53387
                     /locus_tag="PKL17_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43572.1"
                     /translation="MNKITVRKRLFVDGILKWNGQRFGGDDSGVLASLLGGWASGSDN
                     VWIVGEGGTLLHQSGGGWQQVDTQTSRTLSAVTGTADGVFAVGGSGSVLRWDGSSLTT
                     ESTTCATALLGIGQVGADLLSVGEGYCMTRRSGGVWTAVPSMSVPPTDKSGYFSVVGS
                     YQGSSYVSGRPGTLYKLDGTSWSQLELTTMGVARSLLVLPTGDVWIGGSDLYRANGSL
                     PVRLNARPQETLYALSGATADAMWATGFAGTVLRRNGTTWEPVRSDATSPLYGVYSPS
                     ADVAFVIGFDSSESTLSRCSSKACSIIEKASNLEYTSIHGIDADNYWVVGKSGVLKMW
                     SAKSAALVDETGSLPKGASLNHVHAVAGAGGARPSVIAVGEQGLVMRRTAMGWLQDSS
                     APTGKQNLLAVWASADSDVFVGGSNGLIAHFDGSAWAVIPTS"
BASE COUNT         9815 a        15365 c        16672 g        11303 t
ORIGIN      
        1 gaagaaggcg cgttggcctg gcatggggtg cgcgaatccg tccgtccaag tgaaccgttt
       61 gccattatgg aagtgggcgg cgcgacggtg cagcttgcgc tggccgatgc gcactcggat
      121 gatgcccagg tggtggcggt gtccgatgtt cgcggccaag acgtgacgtt cgagcgcttt
      181 accaaaggtg gtccacggcc tgaatttgcg gtctgttaca gccccagtca gcgtcggctc
      241 cagcgcggtc gggcctgcat cgactttcta tacgatcagg tgtttgcgga cgcgcgggtg
      301 acggggcttt cggcggtgac atcgccgcgc caggtctttg cgctgggcgc gccgtggctt
      361 ggccttctgc gtgagctacc gtcgtcgccg ccttttgcaa tcaaggccaa tcgagactta
      421 ccgcagaagg ttcggctcgc cgatttgcag acgctggccg attggatttg tccgctcagt
      481 gacgaacagc tgctggccat tgcgccgcat tcctatgaag ccaagcgcgg ggcgggacga
      541 acctgctatt ccgtcgcgta tcacgctgcc tacttccaag cgatttcgca tgtggcccgc
      601 gccgagacgg tgattcctgg cggagacgat cagtgggcac gcggcgcggc gaccagcggc
      661 ttgttttttc ccgactgtgc aaagccctct gctaaataac cgggcttagg atgcagagcc
      721 tttcacagat gggtcgtcca agtcggctgg gtgcgcggcg ctggcatttc ggtttgctgg
      781 ttgtctgtgg ctgcggaacg ctcggcgacg gtgtgccgcc cccggatgag tggccgcgga
      841 cgcctctgcg cggctttgtg ctgcgcaacc tggaatcgcc gctgctcatc gaaaaagccg
      901 gctttgatct cgatgcgccg acgctggaac ctgatccgag catgccgtcg ctgcgtgttt
      961 ttggtcagct gtggccgcgc aaccagccga cgaagtcgca gctctttgct gtcacgctca
     1021 aaaacctcga tgatcagaaa agccccgacg agctttttgc cgcgacacag ccgtgggaag
     1081 ggcaggggct ttcaaatcca gtctggctgc cgggcggact tggcgtcgat tttccctcgc
     1141 cccttctgtt ttatcagggc agcgatgggt cggttggggt gatgtggcga cagcctgcgg
     1201 gcgatctggt gcgtctgtcg cagccgctcg tcgttgcttc cgatctgacg cccggcaagc
     1261 tgggtcgggt ctcgcctgtg cttttttcag gtcggctcag gctctatttc accgtcgatg
     1321 acagctcgct tcgttatgcc gaagcagatg catcctccgt cgcattgttt ctgcacagac
     1381 cgtcagcctc ggttcgattt gtcagcggtc ctgcgctctt gacggcggcg gatttcaccg
     1441 ttgcggtgtc gcgcagccag caagttccag cggagcggct gggcgcggtg tttgtgcgca
     1501 gcgtgctgac gccggcgggc aggccgcgct atgacctgta tggacgggct gaagcgatgg
     1561 gcaagaccgc gctcgtcgcg gcgtcttcct atcgtggcga tgcgagcgag ccgtttttgc
     1621 cgatcgccga tcctcttttg gccgtcaccg cgggcggcgt tcctggttcc ccgaccgtga
     1681 gtgagtcggg cgggatttcc atcctgtggc ttgggctaag gacggtggcc agtggcattg
     1741 ccgttgctgt gcagcaaaaa gacctgccgc cgaaatcatg agccttcttt ttgcagacat
     1801 cgaagcactg cttggtggtg cggtgggatc ggcgtttccg tcggcttcgc tcagctttgg
     1861 tcagtcgtcg cagccgaaca ccatcgcggt gggcggtgcc agcttggaga cttggtttga
     1921 catcgcgtca ctgaccaaag cgctgtcgac gactttgctg gcgatgcggc ttgtgcaaaa
     1981 gcagcagctc gacttgcacc aagaggtgct gcccggcgtg cggcttgatc atcttttgtc
     2041 gcattcggcg ggttttccgg cctgttttcc gccgctgtgg gcgcagacgt caggtctttt
     2101 ggactcgccg tctgaagcga cgcgacaggc ggtgcttgat gcggtcttgg ctacgccgcg
     2161 cgatcgggcg gggcagcgct cgctgtattc cgatcttggt ttcatcctgt tgggttcgtt
     2221 gattgaacag cgtggagagg cgcggctcga cgatctgctt gcggactggc tgggtccgct
     2281 cgctcttgct ctttcctatc gtccaatcga caagcgcagc ccgatcgctg cggctttggt
     2341 tgcgcccacc cgtcgagaaa cgccccagcg tgagcctttg cgcggtgtgg tccacgatga
     2401 cacggcgcgg gcgatgctcg gggtcgcggg tcatgccggc ctgtttgcgc agagcgcgtc
     2461 ggtgtatcgg ctgggtcagg cgctgctcga ctgctatcac gacgaagaca ccgcggacgc
     2521 tcgtgtgctg ggactgcgcc cagacatcgt gcggcgcttc tttgcgttgc cggcgctgcc
     2581 gggcttgcca gggacgtggg ggcttggttt cgatcatccc gatccgctcg acccgaacaa
     2641 gaccagctcg tcgtcggcag gaacgctgtg gtcgcggcag ggcgtcggcc acttgggctt
     2701 taccggctgt tcgatctgga tcgatccgca gatccggctc gtcgcggtgc tgctttcgaa
     2761 tcgggtcttt gtggcgacca ccgcagaagc agatgcgagc aaatcagccc tgcgtgccct
     2821 gcgtccggct gtccacgatg cgatcgcgcg cttacattgc aggtgagcga aaaggaaaat
     2881 cttgctgctg cgaaacaatt tggtcgggga tagcatctac ccggatgcaa gcgcgatgtt
     2941 tcagtcgctt ttggcctcgg ggtagctatt gctttggggc ctcacttgta aacgctgccc
     3001 ggccaaatga agcttgtggc tgcgtgtttt ccttttagga gtgtaggaat gttcggtgca
     3061 gccagtcccg ccacccagtc tgatcccgtt gaagaagcgc ttcttgggct gctaccgccc
     3121 aaggacgagt ccccggaaac gctgcatcgc gcgatgcatc atgccgtgtt ttccggcggc
     3181 aagcggattc ggcccaggct gcttttgacg gtggcttcgg cctgtgccgc cgacgacgca
     3241 gagatggact tggcgatgcg ggcgggctgt gcgatcgagt ttatccactc cgcgtcgctg
     3301 gttcatgacg atctgccctg ttttgatgat gccgattttc gccggggcaa gccgaccgta
     3361 cacaagctgt ttggcgaacc gatggcggtg cttgtcgggg atgcgctgct gactcgtgcg
     3421 ttcgagattc tcgcggatac tccgtcgcgg ctggcgcgtc gtgcgctgcg gattgttggg
     3481 ctgctcggtc aggcgacggg ttctcagcac ggcatcattg gtggccagag cctggaacaa
     3541 aaagggctcg acggcagcat catcgatcgc tatcacgcga tgaaaacggc ggcgctgttt
     3601 cggctggcgg cgtcagcggg ggcggtggct gcgggcgcaa ccgacgccga ggcgtggggc
     3661 gaggtgggtc agtgcctggg cctggcgtat cagctcgcgg acgatctttg tgatgcggtc
     3721 agttcatgcg aaatcgccgg caagccggtg cgccgggatg aaacgctcgg tcggcccaat
     3781 gccgcgctcc tgaccggcga gagcgtgacc aagcagcggc tggtgtcgct gattctgcgg
     3841 gcacagtcgc ggacccgaga actggccgcc gagcccaagc cgcttcttga gctgatcgac
     3901 gatttgagcg gctattttct caagaccacc caaaaagcca cgccgcaaaa cggctaagct
     3961 ctgtttcgtg aaggacgaac gatcacttcc taccggacga ctgggccggc tgggtcgtct
     4021 ggtttccgcg ggggcgcgcg ccggcattga tctgctcggc aagcgcgatt ccacccacag
     4081 cgcagccgca gcggcggaag tgctgggtac gctgcgtggc ttggcagcca aagtcgggca
     4141 gatggcaagc tacgtcgacg gtgtggttcc cgaacaacac cgcgcggcgt atgaaaccgc
     4201 gatgggtgcg ctgcgtcagg cggccccacg ctcgccatat cccgacattc gcagcctgat
     4261 cgaaagcgag ctggggggtt ccatcggcca gctctttcac acgtttgatg agctgccggt
     4321 cgccagcgcg tcgataggtc aagtgcatcg cgccgtcgtc gcagatgccg cggggaatcc
     4381 ggcgcatgaa gtggcggtca aggtacagca ccccggaatc gtcgctgcgc tcgaaagcga
     4441 tctcgccaat gcatccatcc tcgagcagat gatggcgttt tcagggacgc ggcgcttcaa
     4501 cagccaaggc atgctcgaga tgatccgcac ccgttttcgc gaggagctgg actatcggct
     4561 ggaagcacag cggcagaaac agtttgcgca ggttcatgcc ggcgaccaaa cgattcggat
     4621 tccggctgtc catgatgcac tgtcgagcgg tcgggtgctg acgtcggaat ttgtgcgggg
     4681 cgcgccgttt gaacaagcga tatccgccag cgaagccgat cgtcgggctt gggccgaaac
     4741 gatgtggcgc tttgtcttta agggcaacct cgttggcggc atgttcaacg ccgatccgca
     4801 tcctggaaac tatctgtttg ccgatggggg cgtcgtcacg tttttggact acggctgcgt
     4861 gcaggcgatt ccgcccgacc agcatgagtg ggcggtggcc gttcacaaga ccgcgatcgc
     4921 gcgggatgaa caaggatttg ccgaagcggg caaaaaactg cttgggacca agccggggcc
     4981 gctggaagcg atcgtcctgc gctatctgcg tcgctgcttt gaaccgctgt ttgtgccgcg
     5041 cttccggatg acgcgccagt atgcggcaag ccttgtcgat gacttcaaag acatgacgca
     5101 acaggcgctg aagatggaag cggtctacac gtttgcgatg ccgacggagg ttctgtttat
     5161 gaaccggctc cagttcggct tctattcggt gctggcgcgg cttgatgtgg atgttgacta
     5221 cgccagcgtg gaacgagcgt ttctgaaata gaaaccaccg ccgcaaagcg cagacagaca
     5281 gcgtgcgcag caacatgagc tgcgactgcg tttgtaagtt gtcttgggat ggaaaaacgt
     5341 gaaccagaag cggctcacgg agcaggcagg gaggttaggc gtaggggaga agctcgcgaa
     5401 tcgccttctc gttggtgctg tgcaccactt tggtgccacg caggcggcgc ttgcgcttgc
     5461 tcgcttcgtg ggtgagaagg tggctcttgt gcgcttgctt gtgcttgaaa tgccccttcg
     5521 ccgttggctt gaaacgcttc ttggcggcgg agtgtgtctt catcttgggc atctgagtga
     5581 aatctccttc gatacgaact taccaccaag cctgccgaat cgagaaccta aatttccgag
     5641 gcaaggggaa cttcgagtag gcgcgcgcgg gcttgaaccg cggaccccta ccgtgtcaag
     5701 gtagtgctct accactgagc tacgcgccta agtgcccggg ccaaacgacc gaggcgaacg
     5761 actaatagcg ctgacgactt cgcggcgtca acagtttttt tgagactttt gcttccccat
     5821 ccaaaggatt ggaggattgg cagcggaggt atcgcaaatg ctggtcggca taaactatgc
     5881 gtggcgaaac tatgcgtggg actttggtga accgcctgcg tccgacggtg gcggagcatg
     5941 gggcgcgcgg gcggcctgga caccgacgct gagcgaggaa ctggccgact tcagcgttct
     6001 tggtctcaat gtcgtgcgct ggtttctact cggcgacggg accacgttcg gcgtcggcgc
     6061 tcacaagccg gcgttggacc cagcgcgtcc ggggcagtgg cggttccacg acattccaga
     6121 gctgtcgcct gcctggctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccgcctgct
     6181 catggtgtgc caggacgcca agattcagct tctgccgtcg attgtcgact tccacttttg
     6241 ttttccagcc cagcctgtgc cgtcggcgcc gcttgtcaag tgtggccgca gcgatgcgat
     6301 catcgatcca cgcaagcgtc gtctgctctt ggatcgtgtg gtcgacccca tcattgcggt
     6361 ctgccgtcgc tttccagata cggtctatgc gctggaactt atcaacgagc ccgagtggtg
     6421 taccgacgac ggcgcgtttt ttcacgaaaa gaaagtggtg ccgctggccg cgatgcagga
     6481 cttcatcgcc gaaggcgcgc agcgcatcaa ccgcgccggg cttcgctcga cggtgggttt
     6541 tgctaaacat cgcagcatga aggcgtggga atcaccgggg ctggggctga cgctgcacca
     6601 gtggcactac tacctggacc ccgaggacgt gccacaaaac gacatggctg actttggccc
     6661 gtgcatcgtc ggtgagtttc cgtcggctcg ctggaagccg tggcgcgagc tgggcgacgc
     6721 gcaagacacc cgcagccggc tcgctcactt ggaacgcaga ggctatgacg gggcgctgct
     6781 gtggtcggcc aaccgtgaag aagaaaaaat cgccgaaccc gtcgtcgatt ggtccgagcg
     6841 cgtgcgtgac gaagtggcgg catacaccgc cgggcgctga gcccacgcct gcgcaagcga
     6901 ttctcccgtc gcggtgtttg cttgggcgag cgccgtcggc ggaccggcaa acacgatctc
     6961 tccaccgctt tcgccggctt ctggtcccag ttccaccacc cagtcggctg cggcgatcat
     7021 ttccaggtga tgttcgacga tgaccaaggt atcgccgcga tcgaccagct tgtgcaaaag
     7081 cgcaatcagt cgtcgcgcat ccgataggtg cagtcccgtc gtcggctcat ccagcacgta
     7141 gaccgtcggt tcgtgcttct gtaacgcggt aagctccgcg gccagcttca gccgctgcgc
     7201 ttcgcccccc gacagcgtgt gcgagccctg gccaagctga atgtagccga ttcccagctc
     7261 gcacagcgtc gacagcggtc gcatgatctt cgggtggttg gcaaagtgcc gcaccgcttc
     7321 ttcggcggac agcatcagca catcgccgat ggtcagtcct tgaaatcgca ccgccagcgt
     7381 cgccggctcg aagcgctgac ctgcgcacgc ttcacacggc gtaatcacgt cgggcagaaa
     7441 cgacatttcg ctgacctgca cgccctggcc ttcgcaggtt ggacagcgtc ccccgcccgc
     7501 cgagttaaac gaaaaccgac cggcggcaaa gccccgcact ttggcgtcag gcagcgaagc
     7561 atacagccga cgaatttcgt cccacaccga caaaaacgtc gccggcaccg accgcggcgt
     7621 ccgtccgatc ggcgcctgat cgacgagcag cgctcgcctc accgaatcga tcccatcgag
     7681 cttggcgtga ccgagcggcg cttccgtcga aagcccgagc gctttgcgca gggtcggata
     7741 cagcaccttt tgcaccagcg tgcttttgcc cgaaccgctt acacctgcga cgacgttgaa
     7801 gcgcccgatc ggcagtttca gcgtgacgtt tttcaggttg tgaaccgatg caccgcgcag
     7861 cgacagctgt ttgtcgccaa gcgggcgtgg agtgcgcgtc ggcattggtt ctgccagcgc
     7921 atgcgccgtc ggtgagtctg gatgctttag cacaacggcg gcgggcccct gcgcgatgat
     7981 cgaaccgccg tgcttgccac cggatggacc cagatcgagc agatagtcgg cggcacgaat
     8041 cgtcgcttcg tcgtgttcaa tcaccaaaac cgtcgatccc aaatcggcca agctgcgcag
     8101 gttgcgcagc agccggaagg tgtcgcgcgg atgcagacca atcgtcggtt catcgagcac
     8161 gtacagcgcc ccggtcagcc ctgcgccaag ctgcgccgac agccgcaagc gctgcatttc
     8221 gccacccgac agcgtcgccg cagctcgatc aagcgacaga taaccgagcc ccacctgatc
     8281 gataaaacgg agccgacgca gcagctcgtg aagtggcgca gcgctcagtt tttcttcgcc
     8341 gccttggaat cgccacgtct cgacctgtga cagcgcttgc tgcaccgaat gcgccgtcag
     8401 ttccgcatac gtgtgacctt gcaggcggac tttgcgcggc aatggcgcaa ggcgcgttcc
     8461 ctgacagctt cggcagcgct tgctttcgtc atcggggaca tggccacgac cttcgcaggc
     8521 ttcgcactga ccttgcttgg tattaaacga aaaccagcgt ggatctagct cggggatgcc
     8581 aagcccgcac tttgcgcacg cccgacgcac cgaaaagctt ctttcatcgc tggctgtttt
     8641 gctcgacttg ctgctcccgg tgatgccttg tgccagcgtg atgttgccgt cgcctagctg
     8701 gatcgccaga tcgatttcgg cgcgcgtaaa cagggacggg cggccctgcg cgacgatgag
     8761 atcgatgtgg tgttcggcgg ttttgagcag ccgcggcggt ggatcgattg ccaccagctt
     8821 gccgtcgacc cgcgccgttt ttagccctgc tttgcttgcg gcctggaaga cttccagata
     8881 cgtgcctttg cgtccgcgca ctgccgcggc gtacagcgtg tgcggcacct cgccgagatg
     8941 agcaaacagg gtcatcagcg cttctgtcga agtcgcttgg gctggctcgt cgcagtttgg
     9001 gcagtatggc accccgactt tggcgtacaa aagacgcagg tagtgcgcta cctccgttac
     9061 ggtcgccacc gtcgagtttg ctccagcccg gctgactctt tgcgacagcg caatggccgg
     9121 cggcacgccc gcgacggaat cgacatcggg acgcggaagc tgtggcaaga actgtcgcgc
     9181 gtaaggcgac agcgtttcca tgaaccgacg ctgaccttct gcatagatga catcaaaaca
     9241 gagcgacgac ttgcccgaac cacttggccc cgtgaccacc gaaagaccgc cgtggggcag
     9301 cttgcagctg atgcctcgca ggttgtgttc gcgcgcatgc tcgacggata tcaccgcttc
     9361 ctgggtttgt gccgtcgctt gttcatcggc aaaatgcagg gtcgaaagct gacgcgcttt
     9421 gagcgctgta cccgttcgcg tggacagacg cgcaagctcg gcaggggtcg cctcggcgac
     9481 gattcgtccg ccatcggggc cgccgccggg gcccagatca atcacccagt ccgcctgcgc
     9541 gatcacatcc aggtcatgtt ccaccaccac cacgctgccg ccgttttcac acagcgcatg
     9601 cagcgccgcg acgactggtt cgacgtccag tggatgcagt cccgcgctcg gctcatcaat
     9661 gacaaacagc gtgttacggg ccggttccga cagcgcccgc gcaagcttga gccgctgcgc
     9721 ttcacctccc gatagcgtcg acagcggctg acccagcgtc agataaccca gaccgacgcg
     9781 cagaagtggt gccagcgccc gtcgcagcgc cggatcgggc tcggcttttg ggtcaaagcg
     9841 caaaagcgct tcttcaatcg acagctccag aatctcggcg acgtgtagcc ccagcagctt
     9901 gacggtcaag acctcgggcc gaaagcgctt gccttggcag accgggcaca gaagcgacac
     9961 gtcggcgaga aactgcatct cgaccgtctc gtagccttcg cctgagcagg tctcgcagcg
    10021 gcctttggcg acgttaaacg aaaagtgcga cgcggtaagt cccgccgaca gcgcgctcgc
    10081 ttgggctgcc agtcgggtgc ggaaacgatc ccatgccttg gtgtacgtcg ctgcgttgcc
    10141 gcgcgtcgtc cgtccgagcg gcgattgatc gaccagcacg acgtttttta gcgcctgggt
    10201 gccgctgact tgcttgtgtg cgcctggcct gtcgacgctg ctgtcaccaa gctggcgcgc
    10261 caccgcccga tacagcacat cttcgacgag cgtcgacttg ccagatccgc ttggcccggt
    10321 gacggccacc agcatgccaa gcgggatgtc gacatccaca tcgcacaggt tgttttcgct
    10381 gacgccccgc agggacagca ctttgtcgat cggacgaact tggcgcggct tctgtttgtg
    10441 tttctgccac gccgcgccgg tcggcaagtc ggggcgcgat gccagctctt tgggcggacc
    10501 gtcgaacagc actctgccgc cgtgtcggcc cgcctttggt cccagctcaa tggcccggtc
    10561 acaagccgca atcagcgatg gctcatgttc gatcaaaagc acggtgttgc cgccgcgggc
    10621 gagcgagcgc atcgcgtcgc cgagcttggg cacatcggtc ggatggagtc ccacgctcgg
    10681 ttcatccagc acaaacagcg tccccgtcag cccggcacca agtgctgtcg tcaaactggc
    10741 gcgctgcgct tctcctcccg acagcgtgcg cgcctgcctg tcgagctgca gatagcccaa
    10801 accgacctgc gacaaaaagc ccaggcggct atgcaaagac tcgatcacca gcttgccctg
    10861 cgacgatcgc ggcgaaagcc ccgacagccg ttctagcgat gtggccaccg tatcggcgtg
    10921 ccactcggcc aggttttttt gcccgacccg atacgacagc gccgttggat tcagtcgtgc
    10981 accgtggcac accgcgcaca gctcatacgc tcgatagcgc gagagcagca cccggacgtg
    11041 catcttgtac gtgcgcgatt ccagaaactg aaaccacgcc cgcacgcccg gatacttgcc
    11101 gtcgtcccag gttccctcac catcgaggat cagcttctgc tgatgcggcg tcagcttgtc
    11161 ccacggcttg tcgagcggca cacggtgctt ttcggcaaag ccaaccagcg ccgcccgttc
    11221 ccactgcgag ctttgcccgc tccacggctt gatcgccccc tttagaatgc tcttgctgcc
    11281 atcggggatc accttgtccc agtcgatgcc gatcatccga ccgaagccgc ggcaggccgg
    11341 gcacgcaccg agcggcgagt tgtacgaaaa cagccgcgca tgcggtggtt caaacgtgcg
    11401 cgcgcacccc ggacagtgca ggtctttgcc gaccaggatc ttctctgctt gggtgtagcg
    11461 gctgcgcgcc ccggcgtctt tggcttgtcc gccgcgctcg atcaggcgca cttcggctgc
    11521 cgtgctgtag cggtgacaaa gttccagggc atcgcaaagt cggctgcgtt cctgttccga
    11581 cagcaccaaa cgatcgacaa cgacgtggac ttgcgcggca tcgggcagcg tatccgcttc
    11641 aacatcaaac agttcttcat caagcagcag gcggcgaaag ccttgttcac gcaggcgggg
    11701 cagggcgagc cgcgcttctt cgcttgtgcc gatgggaaac acgataaggg cgctgcgacc
    11761 gctgtggctt tcgcttacgc gggcggctgc ttcaactggc gaaatcaccg tcgcagcaag
    11821 ctggcaggtt gggcagcacg gcaccgcttc cgccgcaaac aacgccgaca gatacggttc
    11881 gacatctgtc agcgtcgcca gcgttgagcg gctcgacttc acaggggcgc gtcgatccac
    11941 ggccactgct gccgccacgg gttccaagct atcgacgtcg ggccgcggca gccgttccag
    12001 gaactggcgg gcatacgggc tgaagctttc gacaaagcgg cgctggcctt cggcatacag
    12061 tgtgtcaagc gccagcgacg acttgcccgc gccgctgggg ccgcacagcg cgaccagtgt
    12121 gcctgccgaa agctgcaggt cgaccgactg aaggttgtga gtcttggctc cccgcaggga
    12181 aattggcagc atcgacgaca tcgtggcttt cctggcgagc tgggctttgg acacctgggt
    12241 gcggcccgcg ctgctactta gatgaagttt gggcagaaaa gcgcacaacg tggttttggc
    12301 aaagcgaaaa aaaccagtgc gaggctcgaa cggcgtcgag cggactctgc tttggcgatg
    12361 tcgggcaggg ggatgtctag cgaagcgtgc gggtcgtcat aagcttcgac agaagcggcg
    12421 ggctgtcgtg cttgggacgg ggtggcatgt acttgtatcg cttgcgccac cagcgcagcg
    12481 gcccgccatc tttgcagccg agcgagcggt ccgtcggcga acagtagatc cgcaggtgca
    12541 agtggtcatc gtgcggcaag gtatcgcccg gctgcttgag gatggcttcg gcgtgctcga
    12601 taagctcctc gttttcgcgc aggtcgcgcg cgtgtttgag cagcacgttg cgcagccggt
    12661 tgtgaataaa caaaaactgc acttcgattt ctgggtcttc caatagcgcc cgtaccagcg
    12721 cccagttgcg acggacatca aacttgcgcg cggtgaaggc ttcaaactgc acgcctggcg
    12781 tcggtcgcac attcaaagag cgcagcccgg ctccatagcg tggcatcgcc gaaaccggca
    12841 tcaccggcgc tccgtcatca tcgatggcgt agtaaatcag gtcggcgtcg tggccgtttt
    12901 cgtgcgagcg atgcagcttg ctttcgccgc cgcccttcat cgcgatgtcg gcgacgccga
    12961 gtgttccacc gggatgctct ttggcgaccc gccgtgccgc tcgcatgatg gcgtttacga
    13021 tttcgtcggt gccgtagttt gcaccgcgct cgcgccacag cgtcggaatg gtgtagccag
    13081 ggccttcttc gggcagcttg cgtccgcggc gcaacgagcc gttgttgaag ttccccatgg
    13141 cgacgctgct cccatcggaa agcatgccgg ggccgatgca gccgaggagc agcagtggga
    13201 ctgccaggag cagaagaccg cgaggggaat tgctgcgcat ggtggaacca ttatgacacg
    13261 cgcgcggctt tgtgggaaag ccgcttgttg ggaattttgt cacccaaacc tgttttctga
    13321 cttaccagcg gaactcactg cggaagagaa tcgcgtgggt aatcacggtg gggacttctt
    13381 tggtcggcaa gtccttgcgg tcatccgagc tcatcgcgat aagtcggtag tcgagcagga
    13441 agaacagcgt gcgccacggg ttggccgata cctggacgtt ggcttcatgc gtgatgcccg
    13501 agcgcttgcc aaagcagccc gaaaacgggt ctgctgacgt gcagaaggct gcgtcgttgc
    13561 cggccagttg atccttggtg atggcggcgg tgtagccgac ggatgccgtg atgcggtctt
    13621 tccaaaagtc acgcccgaca tcgaagctga cgacgtggtt ttgcgcccgg tagtcgaaga
    13681 tcaggctgta gataaggctt gcgcggacgc caaacagtgt gcccgcatcg cgcagcccga
    13741 gcgagacgtc gcccgcaagg ttcgaggtgt tcttggtgta gttcttttgg ctgtagacgt
    13801 cgggaacgct ttcgccgttt atcagactgc gggcgcgcac ccggccttcg acaaagccgc
    13861 caagccgccg gaaaaatcgg ctgtcgagtg tgacgcggcc ttcatcgcgt ccggtgcgca
    13921 gcacggtcag gttgttttcg acggagcttg cgccaatcgc ggccagcgtc gtgccggcgc
    13981 tgcggttgta gaccaggcgc gccaagtaca tgttgatcgc cagcgacgac atgtgcgagt
    14041 agccgaggcg gacggtcaag cgatcatcgg acagcgcgcg aatcgacgac atcaccacgg
    14101 cgcgcgtaag ctgcggacct gccgatccat acaggtccac gaccagatcg ctcatcagat
    14161 cgacgcgctc gtgcggacgc cacgagttaa accagctcaa aaacacccgt ggcgcgtcag
    14221 gcgaatcgct cggtgccacc aggttttgct ggatcggcgc gccatcggtc ggcgagctgg
    14281 cagacaggtt cggcatcacc ggtccaccgt cgccagggcc aaagaagaaa gacgccgccg
    14341 cgccgacact tccccaaagc acatcgtagt tatagcgagc cccggccccc cccacgagac
    14401 cgaagcgacc gccgtcctgc tgacactgct gggtctttag ttcgtctgcc gtcggcgtca
    14461 gcgctgcggc aatcagcgtc ggcgtatcgt tgcgggtgcc cgcgacaccg tttccacatg
    14521 gcgccacgta gtcagtcaaa agcgaccgcg aatacggatt tggatagccg ccaaaaaaag
    14581 cgctgccata ccagtgcgat ccgaagtcgt gtgcgacctt ggcgccgtcg atcttgatgg
    14641 cgtccgtttc ggggatgtac atgcgcccga cttggatgtg ccacgtcgat acaggacgga
    14701 aggtcgcgta ggcttcgcgc agatcatatt cacgcccgcc cagatagcct ctggccgacg
    14761 taccaagcgg cgtcttgtag aactggtcgc caaacagcgt cgtgttggga tcgtacttgc
    14821 gctcgaagtc gaggctgccc gtcacgcgca gccggaagtc gagcttgaag tcgagccgct
    14881 cattccacag ccgagacgcc aaaaccgtcg cgcgcaggtc gataagcccc aggttcgagt
    14941 tggtggcgat gcgcttgtcg atgttttggg tcggatcgac cccgtcgtct tcggtgtagt
    15001 aaaagcccgt gatcgagact ttgccgcgat acagcggacg cgcctccacc cagcttggtg
    15061 cgcacagaag cagcgtcgcg aaaagaagct tccctctcat cggcacaccg agtccgttcc
    15121 caccaggcgg cttacgcgga agctcgcggt gttgtggcag cttccgcagc ttacctggcc
    15181 gtcgtgccga ttggctgcgc ctggaggcaa gccatcggtg gtgattcgca gcgctgcggc
    15241 gcggactgca tcgtcacggt ggcacgacac gcaggccggc gagagacccg cgtagttgcc
    15301 acctttgtga cagtcgctgc acggcaggac acggtgaacg ccggtcagcg tgcagccgac
    15361 tcgatcgtga taaaagcgtg ccccggtaaa tgtctgctgg gtgtggcagt cgctgcatcg
    15421 cggcccaagc gagttgtggt gcacgtcatc gcgctggtgg cagccgatgc acagctgggc
    15481 cagcccctgg agcactcgac cgcttgggtg acactctttg cagaacagtc gatcgtgtgc
    15541 gccgccaaac cgcaccggga agatgtcgtg cccggtgtgg atcttcttcc acgtcgctgg
    15601 ttcgtggcag ccttggcagc ggatgccaag ctcggtcttg tgggcttcgg gatcagcgtg
    15661 gcagtcctgg cactgcgtcg gcgttggctt gtacttctgg gacgggtggc agccttggca
    15721 gcgcaccgcg ttgtgtttgc cgtccagctt ccagcggtct ttgactttcg ggtcattgtg
    15781 gtcaaatgaa atctgacccg tcgggctgtg gcagtcttcg catttgatcc cgaggctgcg
    15841 ctggtgcgcg tcgtctttga ggtggcagcc cgaatcggcg cacttcatcg gcgttggctt
    15901 gtagcggcga tttgggtggc agccttcaca gcgcgcgtct ttgtgcttgc cgaccagcgg
    15961 atagttgctc ttgtcgtgat cgaagcgcgt cgcgccccag cgggcccctt cgtggcagtt
    16021 gttgcaggtg tctttgccga gcgagccttt gtgcagccgg tcttcgtgac acgatccgca
    16081 catcgaagag gtgtcgcggt actgatcgtt tttgtggcaa gtccggcagt ccttgatgcg
    16141 aatgccgtca atgacgtgtc catcgataag ctggaatggc ttgccgggac cgtggccgat
    16201 cttgacccgc tcgttgatcg ctttggggtc gacgctctgc ttgacctcgc cgggcttctg
    16261 gtggcactgg aggcactggt ccgacgaaaa ctgcttcttg tggacctggg tgtgctggtg
    16321 gcagcccatg cagtcgagca cggtggtggt gttctttttg cctttgcccg tggtcgtcgt
    16381 cttcacgagc gcatcgaagt tttcaaactc ggtcggcgtc ttgccgcggt ggcaggcccg
    16441 gcacgatacc gctgcgtgtc gaccaagcag ggcaaagcgc gtctttgtgt gatcgaactt
    16501 cagctcgggg ccccacgtcg tcgataggtg gcacgttgtg cagtcgccgg ttttggtgaa
    16561 gcggccttcg tgcgcatcct tgtggcaagc gtcgcagttt cttcccggtg acttggtggc
    16621 cgtcgatggg tggcacgaag cgcacggttt tttgtgcggg ccctcaagcg caaagcgcgt
    16681 cttgtggttg tggtcgtagt cgatgacctt ccactcgact ttggcgctgt ggcactgcgt
    16741 gcacttcttc tggccgaaca gcgactcgcc gtggacgttc ttgtgacaag gcgtgcagtc
    16801 ggtggcccag ttggtcaggc ggaacgtcgt gcgcgggtgg cagctgttgc aaggaacacc
    16861 ggcatgcttg gtttcgatcg ggaagcgcgt atcggtgttg tgatcaaact tcatcggatc
    16921 gagctggcgc cagttcttgg catcgtggca gcgctcgcag ttggcgactt gctcgtgcag
    16981 ctcaccgtgg ggcgacttgt gacagccaac acaggccatc ggtgccaaaa ggaagctcgg
    17041 acgtccggaa tccgtctttt gcttgtgaca gtctttgcaa tcgaccaccg agtgtcggcc
    17101 ttgcagtgcg aaccgcgtgg tcgtgtcgtg gttgaacttg ttctgcccac cgatggacac
    17161 ccagccgtag aggtcggctt cgaccccgcg gtgttctttg tgacacagca cgcagggccg
    17221 accgagcgct ttgggtgatg cgtgtagacc gatcttctgg gcgatgcgct cggcgatggg
    17281 cttgtggcag tcgagacact tcttttcatc gacctcgcgc ttgccttgcg ggtggcactt
    17341 gatgcagttc gcggggccgt ctatctcggc gtggccacgg gccagtttgc caggagacga
    17401 gtcaaagaag ctggcgcgcg ccgtcgggca ggccaagagc ccgcacagaa gcagaagcgc
    17461 aaacagggcc ctaggcaagc gatggaagag acaagacact tcggcccatc atatcgtttg
    17521 ccgcaggtgc ccgtctagct aggttcgcaa gcgctggttg ggatgtggag ttgcggcgcg
    17581 aattattgct tgggcggcgc gggtggtttg gaacggcggg cgcggaggct ctctcgggcc
    17641 gatcgggctt caggactctg ccaagactgc tgacgaattt cgagcagccg ctccgactct
    17701 tcttcgctca agctgggtcc agagcgcaga aggtgttcaa gcaggtgtcg tgtgccttgc
    17761 accgcagggc gtggcttcgc tgccatcttc tgggccagcg cgcaggcggt ctctagcacc
    17821 acagacgaat ccaccaagcg cgaaatcagc ccaagccgca gcgctgcttc cgctgacact
    17881 ggctctgcca aaagcaagag ctcccgcgct ttgccggcac cacacagcgt cgccaggcgc
    17941 caaattccct cgatcgtata gacgatcccc agcttgaccg gcggcatttg cagcacgact
    18001 tcggagtgcg cgacgcgcaa gtcgcaggac aaggcaattt ccagcccagc cccgacagcc
    18061 ggacccgtca gcgcagccac ggtgggaagc ggcccctcac gcaagagccg cagcgtcgct
    18121 tccagctcgc cgtggccgcg gagccactcg ggatccgggt tggcgggcag cgcatgcaag
    18181 tcatagccag cacagaagac cccgccggcc ccggtgagca ctgccgcgcg ggcaccttgc
    18241 tcggcggctt cgacaatggc ttcgcgcagc gcgaccagaa gccggtcatc gacagcgttg
    18301 gctcgctgca ggtgacacag cgtaagcagc acgatgtcac cttggcgttc tttgcgcaga
    18361 cggcttggca taaaggctcc ttggtctaca tcgtacctag cttgtgcctc ggcgcggtca
    18421 actactttcc gagcatcgcc tgccgaatca tcgcaagcgg cgcaggtccg tagccaagca
    18481 gcgtgtcgtg gaacttgcgc aggctgtagt ttgcgccctg cttcgttttg tagtcctgcc
    18541 gcagcttgag gatctgcagc ttgccgtagg tgtacacgat gtacagcggg tcttgcgtgc
    18601 cgcggcgggc ttcgacttct gccaccgcgc gcatctgccg gccttcgtgg aaaaagaagt
    18661 cggcggcctg cgcttgggtc atgccacggc agtgcatccg aatgccagcg acaaagcggg
    18721 ccgcgcggag gagcgcatcc tgtagctgca tcaggcgaat tttcggatcg cctttgccgt
    18781 agccttcttc cagcatcatc tcttctgtgt agtgcgccca gccttcgacg ttcgacatcg
    18841 tgtgggccag cttttttgct cggctcagct tgccaagcca cagaaactgc acgtagtggc
    18901 cgggaaatgc ttcgtgcacc gtcacgacat cgatcagcgg tcggctgtac gcgccgcgca
    18961 gaaagtcctc ggtctgttcg ggcttccagc ccggctcggg cagcgtgatg tagtagtacg
    19021 cttcgctcgc tttttcgtag ggaccgggcg tatccatcga cgccagcgtc gtcgcgcgca
    19081 taaacggtgg cgtttcgcgc acaagcggca gcacgtcttt tggcaccgtc acgatgttgt
    19141 ggtcgatgag gtacttgtgc tgcgcggctt gtcgtgaagc ggtgtagtca ataagctccg
    19201 acggctgtgg gtggtctgcg ctcacccgct gctgtacgtc ggcagctgag cggcttggat
    19261 cgatctgggc cgcggtcttg tcaaactctg tccgcagccg ggcaagctca gcttccgcca
    19321 tcggcagcag gtcggacact ttggcgtcga tcatttcctc ggcccaaagg gccttctgga
    19381 acatggcttc gccgagcgca aagtcagccg tcgctcgtgc tttgccatcc ttttcgagaa
    19441 atgtaccgag cgccgtcagt gcgtctacga gatccgcact ggcactgcga agctgcgctt
    19501 gcaaacctgg atctggcact tcggaaaacg cttgtacgac gtcatggcga aagaagtccg
    19561 tcgtcgccga tagcgttcgc agcgtcacat caatcgacgc gagcgacatc gaatcaagct
    19621 gcttgggcgc gagccgaatc agcgcgctcg ctttgcgcag ccgcgagatg agcggtcgca
    19681 tgcgtaccgc gagcggcgca tagtttcgct tcagaagcgc gttgatgctg tgcgagacaa
    19741 gcccaatgta cgccatcggc ttgcggcgat gcgcgggcag ctccgaaagc tcgaagcgcc
    19801 gtgtcgcgat cgaatgacgc agcagatcca ggtcgaactg ctcgtcgggc gtaagctgat
    19861 ctttgtgaag cgtggccagc tttcgatccc agtctgaaag ccacgcgagc gtcgataaaa
    19921 ccccttgggg tgaatagtct tccaagtccc cgtcgtggtc gtgcagtcca agctcggttg
    19981 tttcagtcgg atagtggcgt ccaagctcag cgacaaagcc atctacatag gcggacagtc
    20041 gagcgctttc gtctgtcgtt tgggctgcgg cagtcgcggg actttttggc gctgcgtgcg
    20101 gctgtgcggt gaagccgagc agcgtagccg ccaataccag cgaatgtgac agcctgcggc
    20161 tgcgtctttg gctgtggggc gtgacatcca aggactggca aggagtgatg cagaacagcg
    20221 gagctttggt gcgggacatg gccatttttt ctaccaccgg cgcggacttg gcacggctag
    20281 aaaatttggg cggcggcgct ggatcggcat ggactagtct tttgaccttg gacaagatcg
    20341 tgtgtctgta gaaccaaatc gaagaggagt tggcctgtga caaatcgcaa cagtcgctct
    20401 gtcagtttct tccgttccgc tcggtcggtg ctgtcggcgc tggcttttgt ggcactgctt
    20461 ccttccgctg cctgggcaga gcccaaagcg ggggacaagc tgcaaaatcc cgaagtccgc
    20521 gacgcaagag accaaccggc caagctgccg gagtggggaa acaaagtcct cctcatcctg
    20581 tacaccgatc cggatgaagc cgatcagaac gacgagtttg ccgacatggt gaaggcgtcg
    20641 ggtctgtcga aagagtcctt ccagtccatg ggcgtcgcca acatgaagga cgcgcccgcc
    20701 aagccaaact ggatcatccg ccgcgtcgtt cgcaaaaaga tcgaaaagta cggaaccacc
    20761 atcctgaccg accctgatca tctgctcatc aaggcgtgga acctgggcga ctgcaacgac
    20821 aagtccgtcg tcatgatggt cgacaaggaa ggcacgctgc gttacttctt caaaggaaag
    20881 ctaccggagc cagagcgcaa gaaagcgctc gaccttctca agtcgcttat cggcggctag
    20941 tcggttccct cgtcccgttc gcggtttcgg tcttcttctc atagccagtc cgtccggagt
    21001 cccttgtcta gctcgcaaca tagtgtcatc gcgcttgccg gcccgcgcgg tcgtcctcgg
    21061 ctgtcgcttt tgcttcttgc gattgcgctg tgttcggccc tgtggctggt tggtgcgggt
    21121 cgccgaattg attatctggc cgacgattcc tacatcttgt ttcggcatgt tctcaatcac
    21181 agccgtgggc ttgggatgat ctgggacttg tggcatccgg tgcaggccca cagctccatt
    21241 ttgtgggcgt atctgctgcg gctcgcgatg gtcttgggca ttccgattga agacggcgca
    21301 aggattcttg gcgtgtcgtt cggctccggc gcggtcctgg tcttgacggt gacgctgtgg
    21361 agcaagctca acctgcgcag gctgctgctg gctgtgctgc tgctcgcaac gtttcccccc
    21421 ctggtgttgt ggagcagcgc tggcctggaa acgacactgg cgctgtgctt gggtctgcac
    21481 tttctgtccg tagtcggtcg tgcctgtgaa agcagggtcc atccacggat tcgcgatgca
    21541 gagcttatca ccgcggtgct gctcggcgtc gtaagtccgc ttgtgcgtcc ggacttgccg
    21601 cttcagctct tggggggact cgtttgtctg tttatctggc atccgcgctc acgcagcctg
    21661 attcccatgg tcggcctgtc gtgcttgctc ggaatggtcg tctatgtcgc gctcaatcgg
    21721 tcatcgtatg gtgccgcggt ggcagtgcca gtggcgacga agtttcgcat tgaaccgcgc
    21781 aacatcttgc gctttctccg ttacgtccag cgcgatgcgc cacagcttgt gattctgggt
    21841 atcgggctgc ttgctgtgct gcgcaagacg tggcaaagcg agctgtgcca agatcccatc
    21901 tcgctgctct cgctgggcgc gctgggtgcg acgcttatcg agctttcgct tctagggggc
    21961 gatgaactgt cacgcggtcg ctttctgatt tggccttatg cctgcacgct gctgtgtctg
    22021 ggtcgcttgt cgatggcggt gcggattccc aaaggcattc gcagtggcgt gatgctgtct
    22081 ctgcttctgc tctcgtttgc gcagtccgcg tgggcgctgg aaccagaggg tgatcagccg
    22141 tgcagtgtgt ggcgtcgtgc ggcgggaaag tggattcact cccatagtga cccagcgctg
    22201 acgattgccg tggctcctgc ggggtttatt ccgtttttcg ccgatcgcaa cacgattgat
    22261 ctgatgggtc tggcgcatcc gcagattggt cggctgaagc tgccgcgcgg ctcggttgaa
    22321 tgggggcgtc ttgcaacgga aattgcgatc gcggaaggcg tctgcgcggt tcttcttgtt
    22381 gtctgtgact cgccttccga agatccccgc cagcagtgtg cgcatccgac gctccagggc
    22441 atggtcgatg cgcttgttcg ccatccagag tttcgcttta cagaagttcc gataggcgtt
    22501 ggcaatcctt tgcggatggc cgttcgttca gactgtctga agcacttgcg cggaatggat
    22561 cgatagtccc gctacggcgc gcgccggaat ccttccgcgg actttgcaag ctatgctacg
    22621 aaccacgtcc atgagcggaa agcagatcga cttttacttc gacctttcgt ccccctattc
    22681 ctacttcgca gcgacacagc ttgacgcgat tgctggccgc ttcggtgcgc aggttgtgtg
    22741 gaaaccgatg gtgctcgcgg cggtgttcaa ggcggttggc aacgtcatgc cggcggtttg
    22801 tattcccaaa gcccagtaca tggtcaaaga ccttgcgcgg tgggctgcgc actacggcgt
    22861 tccgttccag atgtcgagcc gcttcccgct gccgacgatt cgtgcgatgc ggcttgtgat
    22921 agccggcgaa gcgcttggca aaagcggcga tctagccaag cggttctttt ctgcgatgtg
    22981 ggaacgtgac ctggatattt ccagcgatga cgtgctgcgg cagatcatcg gcgaagctgg
    23041 cttgtctgct gatgcgctgt ttgcggcgac ggataccgcc caggtcaaag acaagctgcg
    23101 cgcctacacc gatgaagcgg tcagtcgtgg cgtcttcgga gccccggcga tggttgtgga
    23161 tggggagctg ttctggggca acgaccggct tcactttttg gaacaaaagc tcgcaaagtg
    23221 aaagaccggc gcgtcggcgt acatcacgca gagaggggcc gactccgttt actttccaaa
    23281 ccacagccct gccgtcaagt tgagcgcgtg gaagtcgtag cttcgtgacg gctctgcttg
    23341 cggacctgcg aacgtggcca agatgttgaa ctggtaggcc agctccagct caaactggat
    23401 gcgctctgtc atcttgatgt cgacgccacc tgacaaccgg gtgacgtgac caatgccgcg
    23461 tcctccgagc tgccggaccc agacttgggt ggcgccgagt ccgtagctca gaaagccgcg
    23521 cacccgcgga ccggggctta tcatgtgctt ggcgaacacg ccgtagtcga tgcccaggct
    23581 gtcggcgacc atcccgccaa agttgtagta gaagccggcc atgaagccga cgtaggaagc
    23641 gtagcgagat gtgaagccgc cgacctgcca gccgaacttg ccatcgacgc gaagtccctg
    23701 cgtctgaaat tttccaccga cattgtctgc cgacagcggc aggtagctaa tcggtgaata
    23761 catccaacca agcgcgatgg caccgactcg tggcgcgtgt cgcatcgtgc caatcggtgg
    23821 ttgcgccgcc tggggcggca gcgggttcag attgggctcg gcaagctgct gaccactggc
    23881 gtcaggctgg acttgacccg gcgggatcgg cgcttggtct ttggccgagg cagttgccgc
    23941 cggtcccatg gacccgtccg gctgtccttg gggctgtatg gatgggactt gcgcacgcac
    24001 agatgggcct gcgagcgaaa acccaagcag cgccacaaga gacagagtcg acttcatcac
    24061 caggatacta atatgttacg tgtggggaat gagcacaaat ccttctcctc cccgccgagt
    24121 tccccgcaag acgcccgtgt ttcccaagga ccttttggat ggtcaggtcg tcattgtcac
    24181 aggagggggc tcggggctcg gcctgtcgat gagcgctggc ttatgccaag ccggcgccca
    24241 tgtggtcatc tgcggtcgca acccggaacg gctggggcaa gccgtcgaga cactgcgcac
    24301 gggcggcggc aaagccgatg ccatcccatg tgacgtgcgc gagccggagc aggtcgccga
    24361 gctagtcaag aaaaccgtgg agcgctgcgg aaagatcgac ggtcttatca acaacgccgc
    24421 cggcaatttc ttgtgtgccg cagaagagct gtcggtcggg ggttttgatg ccgtggtgaa
    24481 gattgtgctc tacggcagcg tgaacgcgac gctggcggtt ggaaagcacc tgatagagcg
    24541 gggtgcgccg ggttcggtgt tgtccatcct gaccgcgtat gcctggacgg gatcggcgtt
    24601 tgttttgcca tcggcctgtg ccaaagcggg ggtgctcgcg atgacgcgct ctcttgcggt
    24661 ggagtggggt atctatggca ttcggctcaa tgcgattgcg ccgggtccat tcccgaccga
    24721 aggcgcatgg tcgcggcttg tgcccgaagc atcaatgata gaaggcttgg ttgaaaagcg
    24781 ggtgccgatg gggcgggtcg gtgaacacag cgagctggcc aacttggctg tgtttctgat
    24841 gagcgacgcg agcccctacc agaacggcga ctgcgtgacg attgacgggg ggagctggct
    24901 tgccggggca ggggagttta gcgagtacgc ccggtacgac cgcgaccggc tgaagacgct
    24961 cctaaaagcg atgcgaccga aaaagtcgta gcttatgagg gcgtcggact ttcgatctgt
    25021 gctgaagtct tgccgaagcg gcgctcgcga tggccaaagt cagccagggc gtgctgcaag
    25081 tcttcggcgg taaagtccgg ccacaggcag tgcgcaaatg caaactcagc atacgcggac
    25141 tcaaaggtga gtgcgtcggc caggtcttgg cctcgccccg tgtacacaat gagatcgacc
    25201 ggcggcaggc tcgcagtggc caggcgctgc tgaatcagtg ccggtgagat ggcttcggct
    25261 ggcagccggc ctgctttgac ttccgcggcc agctctttgg ctacgcgcag caagtcacga
    25321 cgtccactgt agccaagcaa aagcgacagc cccatgccaa gctcgtggga tgacgccgtc
    25381 tgcattgcgt gcagcttgtt ttgaagctgt gccggcaggc aagacagctc tccgtgcgcg
    25441 gaaaaacgca gcgacaagtg gcggaaggtt tcgccgtggc tgcgaagaaa ctggaacaga
    25501 accgtcgatg cttcgtcgct tctgtccatt gggaacgtat ccgtcggaat cagcgtcagg
    25561 tgacgaacgc cgctttgcgc agcggcttcg atggctcctg cggcggtcag cgccgacgcc
    25621 cacagcccag agctgggcga tagcccgcgc tctgtcgacc agagtctaca gccatccatc
    25681 aagatggcga cgtgatccgc tcgttttttc atcgaactaa gctccgtcca gaatgtaaga
    25741 gcgagcagtc tactcaagtc cgcgctcgcg taaacgctac gatgcgcatg ccgcgtgaag
    25801 aatgggttaa cggcatcgga aacttcgatg aatttcccga cgacgcacgc tggaccatag
    25861 ccttttgtcg tgtgctgcga tacacgtcga aacatgtcga gcttgaaggt cgaggagcgt
    25921 tttgtagtcg atgcggacgt gctgcgggtt tgggaattcc tcaagcggcc cgcgctggtc
    25981 gccggctgct tacctggcgc caagctggat ggacaagagg gcggggacac gttccttggc
    26041 acgatgaaga tcaaggtcgg tccggtgatg caggactttc gcggcaaagc gacgctagcg
    26101 gaagtcgatg acaccttgca caagatgacc gtaagcgggg ttggcgatga caaggccggt
    26161 ggtggatcgg cccgcatgac gatgaagtgt caggtggtgc cgagcagcga gaccggcaaa
    26221 agtgaagtaa ccatcgtcgc ggaggtcgag ctattcggca agctggtgcg ctttggtcgc
    26281 gggatgtttg aagcggtcgc caagcagctc tttaagcagt ttgtcgatcg tgcccgctcc
    26341 gcgattgcga gcatgccggc tcccgctggg tcgtcggaac cggcggtggt ccttgcgaca
    26401 ccgcttcccg ccacaagcac cggaacaagc gaagcaagca ccgggacaag cgaagcaagc
    26461 accgggacaa gcgaagcaag caaaccgaca agcgaagcaa gcaaaccgac aagcgaagca
    26521 agcaaaccga caagcgaagc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nagtgcggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gaagcaagtg
    26761 cggcatcaag cgaagcaagt gcggcatcaa gcgaagcaag tgcgcggaca agcgacgcgg
    26821 gtacgggttc aaaggatgcg agcacggctt cggcgtctgc aagtcaagcc gcaagcagca
    26881 aaagtccgcc agctgtcgct gcccaatcga cagctgcccc ggtcgtggcg acccccaatc
    26941 agcgcgtcgt caagtcgaac gatgacgaag ccctggatgc agggtcgctg atctttgcgg
    27001 tgatttggga gtggataaag ggtctgtttc ggcggctttt cggacgcaag tagccgcagc
    27061 tgaggcgaga ttacttcttt ttgccggtca gccagttcca gccgttggaa atcgctgagc
    27121 cgacgttgtt tgccacattc ttggcggtgc cgacggcgga attgacgccg ttcgagatgc
    27181 cggtgccgac cttgctcgcg gtggatttgg cgccatccca ggccgaactg acaccatttg
    27241 agatcgcgcc gccgatgctt ttggccttgt cgatggcagc gcctatcggc ttggtgatcg
    27301 gcgcggccat ttcagcgact tttttctgaa cgctgggatc gtgtgccagg tgattgacga
    27361 tgccgggcag accggacgca atctgtttga gatcaccgcc gtgcggtgcg atgtggtcgt
    27421 ggaagaagtt gccgagcgtg ctggcgccgt tcttgacgcc ggaccacatg tcctgcgcgc
    27481 ctttgcccat ctccttggcg ccactccagc cgctgctcag gccgtggccg agctcggaca
    27541 ggcccttgcc aaagttgccg tgcaacaggt cggatacgcc gtggccggct tccgttgcgc
    27601 cgctcttgac gccgctccag ccgtctttgg tttcacccca gccgcccttg gtctgattca
    27661 ccgcgttttg cagcggaccc ggaatcggct tgccatcttt gccgagcacg gggttccact
    27721 ctgagtcggt cttctgccaa aggtccattc cagcgccgac ttccccgagc aatccggaag
    27781 ccgctggtcc gagcgtgcgg cgctgtgcct cccagaacgc gcgatcgctc tggcggtcgc
    27841 cttggtagga ctggatggtc gtgttttgat tggtcgcgtt ggcgtagccg tccttgtctt
    27901 tgactttgcc gtgcgcgtca atcgtgccct tggctccgtc cgacggaatc aggaaggacg
    27961 acaggtgcgc atctccggtg tttagtccgg ttgcccagtc gccgcgggtg tattcgtggt
    28021 agttgccgtc gactttcgct tcgccggcgc ggtgaacgat gtcgttggta aagaagtggt
    28081 gattgacgtc gatgccgtac ttcttttcgg cggcgttgac ggcggcggcg tcttggccgg
    28141 cgatgcccgg tgactggaaa gtcgagagcg agcggacgtt ttgcgcattg tcgacaaggg
    28201 atcgctgcgc cagggcgcca ccgaggctgt atccgacgac atcggccttt tcccccggct
    28261 tgtggccaaa cagcttggca atctcggctt tgccgggctc gtactccgac tggccgatgt
    28321 acttgtcacc caggtcggct tgggcatcac gcgggccacg gtcgggctcg gtgccgcgga
    28381 acgcgacgac cggtgccgtc ccatttttgg caccttgggc cgatggctcg aatcgaatcg
    28441 caaacaggcc ggtgtctttg ttgtgatcga cgacgttgct gggatcgacc ttgggattgt
    28501 agccccacgc cgatagcgag ttctgctgcg ccgccgagta gttcgagacc gggttgccat
    28561 ctttgccgac gaccggcttg ccgtccttgt ctttgacaaa cggatcagtc tggcagagcc
    28621 gcgccatgta ttcgtggacg tactcttgct tgtactgctc cgcattcatg ccgagcgctt
    28681 tggctctctc gaagtcctgg cctttgcctt ccttgaactg ctgttcaatc tgctgcggcg
    28741 tcaagatggc ctgatcaggc ttgccgcctt tggcatcttt gccggatgcc gcgttgccaa
    28801 cggcttcttt gatggcaggg ctgccctgtt tgtcggggtt tagcgtgcgt tcttggccct
    28861 ggccttcgcc tttttggccg ctgccgcctt cgtgctccga ccactggccg ccaaagccac
    28921 ctccggtcag ccaaggcgat gcatcgccca gctcaccaaa gtgaccgccg ccggcttgaa
    28981 aaccctggcc gacgccagca tcgcccagct cgccaaagtg ccctccgccg ccgaatccac
    29041 cgccaagagc ccatggactt ggttgctgct gttgcgacgt aaaaccggct tcgcctgaca
    29101 tgtggttctc cgctggctgg ttgcttttgc catagagttt tcggcagagc gaccgcggcg
    29161 ttgccgcgct tcagcgaact tcttccaggt cacgatgtag ggtttttttg acttagtggg
    29221 ttgctgctgc caaaaggttt gcccgccaat caaggaggcg ctcgtggccg cgactcagta
    29281 catcgtcgag ggaggacatc gcctgcacgg aacggttcgt ccggcaggaa acaaaaacgc
    29341 agcgctgccc atcattgctg cggcgcttct cagccaggac aaagtgacgc tgcgcaatgt
    29401 gccgcgcatc cgcgatgtcg agacgctggt cgacctgatt cgatccgtcg gtgctgaggc
    29461 agagtgggac gtaagcgaca ttcataccct gcacattcac gcaaagacgc tgcgcgccgc
    29521 gtcacttgat cccgacctgt gtgcacggat tcgcgcgtcg atcttgctgg ctgccccgct
    29581 tcttgcacga tgtggggaaa ttcatctgcc accgccgggt ggtgatgtca tcgggcgacg
    29641 gcgtgtcgat acccactttt tggcacttgg cgagctgggc gcgacgtatc agctatcgga
    29701 gcggtttcat ctgaccgctt cgaagctgcg tggcgcagac gtgtttctgg atgagccgag
    29761 cgtgaccggg accgaaaacg ccatcacggc tgccgtggca gcgaccggca ccacgatttt
    29821 gcgcaacgcc gcttgtgaac cgcacgttca ggatctttgc aacttcctgg tgacgctggg
    29881 tgcgcagatt gaaggaatcg gatcgaacca actgacgatt catggcggac gcgcgctgca
    29941 cggtggggag ttttccattg gccccgacca catcgaagtc ggttcgttca tcggtctggc
    30001 ggcggtgact cgatcggagc tgcggattgc acaggctggc gtccgtcact tgcggatgat
    30061 tttgcttggc tttgagcgcc tgggaattac ctgcaaggtt gacggtgatg acttgatcgt
    30121 gccgagcgcg cagagcatgc agattcggtc agacttgggc ggtcacgttc cgaagttgga
    30181 agaccagccg tggcccgcat tccctgccga ccttctgtcg attgccattg tcaccgcaac
    30241 gcagtgtgaa ggcgtcatct tgatgttcga aaagatgttc gagtcgcgcc tgtttttcgt
    30301 cgacaagctg gtgtcgatgg gggcgcgtat catcctgtgt gatccgcacc gggcgctggt
    30361 gtatgggccc tctttgttgc gtgcgggaac ggtcgaatcg cctgacattc gggctggaat
    30421 ggcgatgctg attgcggcgc tgtgcgcgca gggcaaaagc acaatcctga acgtcggaca
    30481 gatcgagcga ggctacgagc gcatcgtgtc acgcctgcgt tcgctcggcg ctcacatcga
    30541 ggaagtcgaa gaccgtcgca gtcgccgcta atggtggccg cttcgcgtac aatttgctga
    30601 ctttttcgcc ccaaaagccg ccgagaacgc tacaagatag ggcttctagc gaaaggcaat
    30661 tgggtgaagg tctcgatcat catccccgtg tacaacgaag agcggacgat tggcgcggtc
    30721 atcgaccttg tgtcgtcgct cacgtttgac aaggagatca tcgttgtaaa cgatggctct
    30781 agcgacggaa cccgcgaggc gcttcgtccg tatgagagcc gtcctgggct ccgcgtctac
    30841 cactgcccgg tcaacctggg caaaggctcc gcagttcgca tcggctttag catggccgaa
    30901 ggcgacatcg tgacgatcca agacgcggac ctggaactgg acccgcgcga ctattaccag
    30961 ctcatcgagc cgatcgtgtc gggccaagcc gacgttgtgt acggttcgcg ctttttgagc
    31021 caggacggca caagccatcg gcgcggcatg ctgatgttct atctggccaa ccgcgcgctg
    31081 acaggctttg ccaacctgct gtttcacggc cacctgaccg acattgaaac gtgctacaag
    31141 ctgttccgtc gcgaagtgct gcccaagcta aagctgcgtg ccgctcgttt cgagattgag
    31201 ccggagctta ccgcgcaggt gctcaagaac ggctatcgaa tcgtcgagct gccgatctct
    31261 tacaagccgc gtacccatgc tgagggcaaa aagattgctt gggtcgatgg ttttatcgct
    31321 gtttacaccc tcgccaagca gcgtttcggt tagcgcaaac cctgttcggc ctgcgcgcaa
    31381 tcagtcagca tgagtgatgc tgagcatctt gctcggcgtg cttcatctgg gaggggaccc
    31441 gatgcgtgct ggacgaaaac agcgatcact agacgtttgc ttccttgctt atttgtttgc
    31501 ggtgggatgt acgagcagtg ggaccagcgg aaacaccgat gccgccggac tgacggacgg
    31561 cggggacggg gatctggcga tggcaacatg ttcgccagaa ggtgtgtttg atggtgctcc
    31621 ggtcacggct gccgctggac agtgggcctg ggtcgatgag cccaaagcgt tgtgccgtga
    31681 tggctctgcc acgggctttg gtgtgcggat caatccaaac tccgacaagc tcgtgatcta
    31741 cttccagggc ggcggagcct gcttcaactc ggcgacctgc ggttcgaatc cggcgacctt
    31801 caactcgggg acttttgcgc tcgcggtgct tgctggtggc cagcagggga tttttaacca
    31861 gacggattcc gccaatccgt ttcgcgattg gaatgcggtg ttcattccgt actgcacggg
    31921 cgacgtgcac gccgggaccg caacgggagt cacggtgccg ggtgtggggg cgcccacgaa
    31981 tcagcagttt gtcggctacc gaaacgtggg ctactaccta aagcgcatca ttcctacgtt
    32041 cccgaaagtg acacgggtgc ttttgaccgg cgcgagcgcg ggcggttttg gctcgatgct
    32101 gaactaccat cgggtggcac aggcgttctg tccgcgacct gtctcgcttg tgaacgactc
    32161 aggtccggtg tttcaagatg catttctggc tccgtgtcta cagaagcgct ggcggcagct
    32221 gtggaacttt gatgcaaacc taccagcagg ctgcaccggc tgtagcaatg cggacggcag
    32281 cggaatggcc aactacgaaa agttcatcgg ccaaaattac ccggcacagc ggctggggct
    32341 gatttcgtct ctgcaagacg cgacgatttc ttacttcttg ggctttggac agaacaactg
    32401 ctctgcgatt gatggcacgc cgggcccgct acctggcgcg acgttccaag cgggtcttat
    32461 cgatctgcgg gacagcttgc tcaagccgaa tccgaattgg tccacgtact acctgactgg
    32521 gacgacccac acgtatctga acaacgcgaa ctactcatcg cttaccagcg caggggtcaa
    32581 gctcacggac tggatgatag gaattgccaa ccaggggcca tcttcccacg tcgcaccgta
    32641 gtccgccttg gccctgtttc cgcgcaagct gcgcctgctt gtttacttca gcagtggcag
    32701 cgtcaccgac gaacgcttgt ctccgccgcg atagacttgg tgggtcgtga ccaggaagtc
    32761 cttttcggtt gcgcgataga tgtcgacaaa ctgctgtggg ttgcgatcga ccagcggaaa
    32821 ccacgagctt tgcacctgaa ccatgatgcg gtgtcctggg cggaaggtgt ggaacacgtc
    32881 cggaagctgg aagcgtatgc gcgtgggctg gttgggcgtc atcggctccg ggcgctcgaa
    32941 gctctggcgg aaccgagcgc gcatcacttc gccgcgcacc aaggcctgat aaccacccat
    33001 tcggacttcg cgcggattcg gttctggatc ggcggcatgc tctggataga catcgatcag
    33061 cttgacgaca aagtcggcat ccgttccggt cgtggacacc cacaggtcga catcgatcgg
    33121 gccagccacc gtcacatcag cgtcattggc cggcgcgaca taggaaagaa catccggtct
    33181 gcgtgaagca aagcgctggt cttcgaccat gtattccgga tcgcgatggg cagacagtcg
    33241 ctcgcgatag ggaaccggtt tgtgtggatt gctgatgtag cgatcgaacg catcgcccgt
    33301 cttgctgttt gggtcgatgg acagtccgcc gccttctgca aaccaaaagg gagtcggctt
    33361 ggctgtcgca ggcggccagc tttcatggct gcgccatacg ttctttcctg tttcaaacgc
    33421 ccacgctttg ggcggggttt ctgcgctgat tcctttgaga tggtgaagga aaaaaggcac
    33481 cagcatgtgc tcttggtagt agcgcgaagt cttggcacca aagtggatgt cacccaggga
    33541 atcaccgtcc gttcgatact gacctccgtg tttccatgga cctagcacca atgtactttg
    33601 cgtgctcggg ctctgttttt cgatcgcatg gaatgttccc agcgttccgt acaggtcttc
    33661 ggcgtcatac aggccgccaa ctgtcatgac tgccggacgg atgttgcgga aatgcggacg
    33721 tggatcgcgg gccttccagt agtcatctcg attcggatgt tcgacgcatg ctcgccaaaa
    33781 tggcagcgcg gtgccaacaa tccttgtgtc ggcgtcaaac agcggcaaca ccgttaggaa
    33841 gtagtcgtac aggtcgccgc tttcgagatc aaagtcccag cgagctttgg gactgggctt
    33901 gggccgcggc ttgccgaagt ttgcgtaaaa gagaaacgta tcggcgagaa acagcgcgcc
    33961 gttgtggtga aagtcatcgc ccatgtacca gtcggtcacg ggcatctggg gcgacaccgc
    34021 tttgagcgcg ggatgcgcat caatcgccgc ttgtgccgca taaaaccccg gataagagtt
    34081 cccccagata ccgactttgc cgttgttgcc tgggacgttt ttcaccagcc aatcaatggt
    34141 gtcgtaggca tcgctcgact cgtctacatc cttggctgtt cgcttgtttt cgatgtaagg
    34201 cgtgacgtcg gaaaagacgc cttccgacat gagcttgccg cgaacgtcct gctgcacaaa
    34261 gatcatgccg tgcgcgataa gtgccgtggc tggcgccagt cctcgcagct gacgaggttc
    34321 cttggcagaa ggatagttgt cgactccgta aggcgcgatc ccgtagggcg tgcgggtcat
    34381 cagaaacgga tagcgtcgcg agctgtcttt gggcacatag acgtgggtga acagtcttac
    34441 gccgtcgcgc atcggaatgc ggaattcgtg cttggtgtag tgttctcgta gggcgcgagc
    34501 gatgtcttcg tcgcttgcaa gctcgcgtgg gataagcggt ttgtcgttgt ttgattcggc
    34561 tgcgggcttg ggcggtgcgc ccaccgcatc gggtgccgtg gaaagcagaa gggaaaacag
    34621 caaggttcgg aatcttcgca tcgcagaaat agagcgtgta gcgcgtcgct ggtcaaatgt
    34681 tcggcacctg catcgtgagc cgggcttgaa tccagcggtt ttgccgatgc gacggtgtct
    34741 ttgtctgcca cgccgctcct tgcggtgatc gactcctatt tggctgcaat cactccgcat
    34801 ccgacacggc ttccggagtt tccggtcggc tgggttgcgc agtcatctgt accaccgtgc
    34861 aagatgatgg ctttgccaac gacgctgcgc gcacccgcgg acacggtgag atccttgctg
    34921 acgagctgca atacgccttt gccaccggag atcgtgatgt ttcccaagtc gccggcgtga
    34981 tgaggcgttg cggacggaca gccgtggttc acattgtccg gattgaagtg gcctcctgcg
    35041 gccccgtggt gaacgccgcc atcccctgtg gtgtccgcgc aatcgccggt ggcgtggatg
    35101 tgcattccga aattcccatc ggtcggaacg cccgtgatgt tcacagtgat tttcactccg
    35161 tcggacgttt cctcgaacgt cgcggttcct tgcgcaccgc cgggttcatt tatctgcgcg
    35221 acggcttgcg gagctgccat gtcgacagga ccactggttc cactgttaca gccatactcg
    35281 gtaagcgctg agcaggttgt gatcagaagg accgagaggc gagaaaatgc gatgcgcttc
    35341 atcttgagcg ttctcctttt gtggttagct gctgcacgac agactggaac agccgggacg
    35401 atgccgcgcc cactcggggc gcttggcggc atactgtgac catgtgggat tgtcatcata
    35461 gggattgcgc aacacatcca gcagttcgta cacaagcgag ccgtcgccct gttccgcgcg
    35521 atcaatcgca agctgagcca ggtagttgcg caggacaaca tgggggtttg ttgcgttcat
    35581 cgcagcccgg cgatcttcgt cgcgcagcgg ctgatcggaa atgcggtgcc gataaatcgc
    35641 caagaaatcc aggatggctt cccgatgcgg acccgaaaga tcctgcggat ggtagtaagc
    35701 gtcgctgagt gtctcatcaa ggaactgcga aagattcgct tgatcctgac ggagcagggc
    35761 gagcttgcgg aagaacacgg tcatgtctgt ttcgaccaac tggaagactc tgtagagttc
    35821 tttttccagc gcatcgtcac ttgtgggtcg ccaggttgta agtcccagct tgcgcatcat
    35881 catcgcttgc tgctcgcgtt caaacgtgtg gggatacgcc agcagggctt ctttgatagg
    35941 cgctgcatct tcgatgagtg gatacagcgc ttcggccagg cgaaccaagt tccaaaatgc
    36001 gatgtgtggc tgattcccaa aggcgtagcg gcggcccgct gcgtcagtgg tgtttggcgt
    36061 ccacatcgga tcgtagtctt ccagccatcc gtaaggcccg taatcaatcg taagtcccaa
    36121 gatgctcatg ttgtcggtgt tcatcacgcc gtgaacgaat ccgacccgct gccactccac
    36181 catgagccgc gccgtccgtt cggcgatcgt tcccagaagt cggacatagg aatcgcgatg
    36241 aggccgaccc agatccggaa aatgatgccg cagtgtgtag tcagcaagaa gtcgcagcct
    36301 gtcaacatcc cgtcgagctg tgaagatttc aaagttgcca aagcgcaaaa acgtgggcgc
    36361 gacgcggcag acgatggcac ctggctcggg ggcagggtgg ccgtcataaa acatgtcgcg
    36421 gatgacgctg tctccggtcg cgaccaagct gagcgcgcgt gttgtcggca cacccaagtg
    36481 atgcatcgct tcgctgcaga gaaactctcg cacggaggaa cgcatcacag cccggccatc
    36541 ggcgccgcgg gaatagggtg tcggcccggc tccttttagc tgcacttccc agtgcgttcc
    36601 agatgacgtc agcacttcac caagtcccag cgcgcgtcca tcgccaagct gtccagccca
    36661 attcccaaac tggtgtccgc cgtaacaagc ggcgtaggga gtcattcctt ccgccagtgc
    36721 gcagccgccg aaaatttcgg caaatgttgc gtgctcctgc gcgtctgctg aaagtccgag
    36781 cagcgcggcg acttctttgg cgtgggcgat gacgcgcggc gatgaaactg gcgtgggctg
    36841 caccaaggag tagcaagcgc catgcacttg tcgccgctga ttggtctggt cgggatcgtg
    36901 gggaagctcg cgaatgaagc gattgtcaaa gcgcagcgcg gcaaacggag tgtctttgta
    36961 cgtcggcagc ttgcgggact catgtgcaga gggcagggga gatgtcggca tgtgtgtggc
    37021 tatttgtaca tagatgccgt ggcggtactg tccgtgccat ctctttggtg tcgtggtgta
    37081 tgccaagcgg aaggcgtcca tcggcccgct ggttagaatt tcccagtgaa agcgcaggca
    37141 agaagtgcaa ggtctttcga ttcggggtca catctggcta cgatgatcga gtgggggtgc
    37201 ccgtgatgcg actgacttct gtgatcatgt ccctttttgt agcgagctgt tccacgacgt
    37261 gggatgcatt ccgcatcgca aaccaggatg cgtgtaccaa tcatccgctc gactgtggaa
    37321 gcggcaaagt gtgcagctac gaaacgcgcc agtgtcagcc gcagaactgt cggcaaagtg
    37381 gattctgttg ggaaagcccg cgtccgcagc tcttttcccc acgagcgatc tatggctcga
    37441 gtgcaagtga tctgtggatg cttgggacgc gggaaatcta tcgcgcaaat ggttcgacga
    37501 tcacccccgt catcaccgaa ctacccgtgc gcatgaacgc catcgttgga atcgcgccag
    37561 ataacatcct gtttctggga gaacagggaa ccatcctttc ggccaattac atcgctccca
    37621 cggtgtcggt tgatgaccta cggatggcat cgtaaagaga gctatgtaac gaccctacag
    37681 gcgcgagctt gcggacatta cagcgcgaac tgcgcagtac ggacagcgtg gggccaagcg
    37741 tgcccgcgct attagggagt gccaagaatt cggacggggc caaggccttc ttttttcagc
    37801 tgcggttcga tgactgcggg gtcgccagcg atgacaactt cgagtgtgtc tggcactacg
    37861 tagtcccgca gcgctttgta aacttcggga tggctgacag tctggatttt ggcgatgcgt
    37921 ttttgccagt gatctgggcc gtaccggaag atcccttccg aaagcgcgcc gataccagcc
    37981 agcgacgcag attcctcggt gatatcgcgc accatatggg cacgatacat cggcaacagc
    38041 ttgctgttta ccgtatcgct ggtggcggca aagatttggc ttaggacttc gcgcgcggcg
    38101 taacctgtgt gctgcgcttc gactgctgcg gtgacgacaa acactccgct ttgggccgac
    38161 ttttggtagt gtgcagacgc accatacgtc acagtctttt cctcgcgcag ctgttggccc
    38221 acgttggtgc tgacccagcc agcggccatt tcgagtgcag gtgcatcgac gtgtccatca
    38281 gatggggcac gcctgccaat gaagatgacg ctctgcacaa gccccggata gggcacaaca
    38341 taggaagccg ttcgcttttc gggcttggga atgggaagca cgggctgcgg aacgtctccc
    38401 tccggcagac cggtcagtga tttttttacc cacacggcag cgacgggtgg tgcaattcgt
    38461 ccggcgattg cgatcgcaag attccggctg acgacaaagc tgcgatgtag gactttcagc
    38521 tcttcaaggt tgcgcttttt cacctctgcg gcttcgtcct ttggatttcg gcacagcggg
    38581 tgatctttgg gaaacagaat ccgattgagc gcgagcctgg ctgccgcatg cgggctctgt
    38641 gttttggtcg ccagttcacg atagagggac tgctgcgcat tttttagctc cgcttcatcc
    38701 cacttgggtt cgacgagggc gcgcagcact gccgagatgg cactggtcgc atcctcggca
    38761 cggacttgca ccgcgaccga tacggcgtct tcatctacag cgagcgatgg cgtggctccc
    38821 agctcgtcga gtgcagacaa aagagctcgg ttttccgaaa ccaaggtgcg aaacaaaaga
    38881 cgaagcgcgc cgcgctggtt tgccggttcg acagcgagtc cagacagaaa ctgaacccgg
    38941 atatgaaaca gaggcagata cgcatcggag tgaacaatga gctgcacgcc agaaggcagg
    39001 atctgccgtt ccagctgtgg catccggatc accacatcag agctgggcac cgggcgattg
    39061 agccggaagt aatcaaaacc ggtcgcccaa aacgccaata agaggagtgc gaagcgcttc
    39121 atttcttaga ctcctgactc tgcggcatgc cgaaggtgac gacgcactgc ttttgcccaa
    39181 ggtaggcttt tacaaatttt tgaatgtctt cgctgcgtac cgcttgaacc tgcgccagag
    39241 atcgctcgac gccctgcggg ccaccgtggc agcttatcgc acgggccaaa tggaatgcct
    39301 tggccagcgg actttgcaga tcggacagcc aatcgtttac caacttcttc tttgcagcga
    39361 caagctctgc atcggtgacg cctttttccg ccagttcttg cagctctttg ttcacaagct
    39421 ctttggcgcc ttctagcgtc ccccgatctt tggcggcgat gtgaaccgtg aacacagact
    39481 ggagcctgtg agagtcctgg cttgcgccat cggacagaat ctgctcgggc cacttttggg
    39541 acagcttgtg cagtcgtcct tgttttccgc gcgacaggat tgtcgcaagc acatcggcgg
    39601 ttcgatcctc tgcggagcag gtaggcggcg tgagccagcg caaaagcagc acgggctcct
    39661 tgagcagcgg atcatcgtgc gacagtacag cttcttcgct gactgtgacg ggctttggat
    39721 cgggtggggt gttgtctttg ccgtgacttt ggatcgtccc aaagtacttt ttgacaagtg
    39781 atgccgccgc ggttttgtca atgtcaccag acagaatcag cgtgacattt tttggatcgt
    39841 agtacttgcg gaagaacgcc tttgattcat ccagagtgag cttgtcgagg tctgtctgct
    39901 caccgatgac cattccgaag tacggatgcg gagccggaaa cagcctcttg tgcgacactt
    39961 catcggttcc gccatagggg ttgatttcac gtcgctggcg gcgttcattt ttgacgatct
    40021 tgcgttcgtc ttgcagatcc aaatcccgca gcacagggaa accgagccgg tctgattcca
    40081 tccacaaggt cggttccaga ttctcactgg gaacaacctc gtaatagatc gtttcatcaa
    40141 ggaacgtgga gccattcatc gaccgcactc cagcggtctg cagataccag cgcgggctgt
    40201 acggcaaaag gtgctctgag ccatggaagt acagatgttc gaacaagtgc gccagcccgc
    40261 tgcgtccgag cgcttcgtga cgagcaccga cctgcacttg cagcatcacc gcaacactcg
    40321 gctggcgacg gtcgacatgc aaaaccgttg tcattccatt ttccagcgaa agtacgtccg
    40381 caggaatcag cgactgagcg gaagcggtgt gcggaagcgc gaagaccgac gctcccagaa
    40441 tcatccagtg aggccacaga gacccgcttg gtttcggcat aaagatcacc tatttccaga
    40501 aatgtgggaa cgacactggc acgacgcaag tacgagcgca cgtttcggat cctgacaacg
    40561 ccgatgcgat tttcctgtca tgctggtggt ccagacgcag cgttatgtag cgcgagcgaa
    40621 tcggacgatg caagcatggc ggcgtctgct aagtgatgcg tagagcgaac cgacttgggc
    40681 gagacatggc tgcggtgctg tgcgcgtaaa ttgcggcatg gcttacggta tccagaaagt
    40741 tgcccagcct cgggtcattc cggtgatcat cgttgtatga agaagcgaca gactttgatc
    40801 ggactgtttg tgttttgtgc gtgtcaggcc agcccgcctg tcgagggtgg caatgtgggc
    40861 tgcaccgata gcaccacggc ttgcattgcg gcggacggct gctgcccagc cgggtgtaca
    40921 agcgccacgg attccgactg tccggtgccc cccgagaccg cgctggccga tcgtctgcaa
    40981 gttgtcactg tgaccgcgcc ggcgggggtg attccgggcg tgtcaaacta tcgaatttgg
    41041 ggatcgacca gcttggtcgt ggggccggtg tacacggttc cgtttgcgga ctgcggcacc
    41101 ttggtgggtt acacgacagg aaccgttgcg gcaccgcgag cgcgcgtggc gcgtctcgat
    41161 gcgcaagaca agctggtgac gacctatgac ttgggggcgt ttacggtgcg gggtctggct
    41221 gcggagcccg atggtcactt cgcggcgctc ttgtgggatc aagcgacgaa tcccaagtcg
    41281 ctcagtgttt cgcgctatga cgcgcaggga atggccggat ggacagcatc gctggccgat
    41341 ccgctggccg caccaacaga ttttggcatt ggagagagcc gactggaata cggcgcgggc
    41401 aaatacggtg cgtacttcca tgtgcatggg atttctggtt ttgcaaacgg acacgaaggc
    41461 gatgcgctgc actgggttga tgccgcgagc ggaatgcaca ccgtgggctg gaactggggc
    41521 tgttcgcact cgatgagcga gcttctgcgc tatggctccg ctgcgaacgc gacgctggcg
    41581 gtctgtgtaa cggactgtta tccgggcacc acgggcagca catttgccac cgattcgatc
    41641 ggcggtgtgt atctggaaaa agcaaccaaa gtgcgtgact tcgatggcgg ctgtaacggc
    41701 agcgtcgccg cagagctggg cagcgcagcc cctgggctcg caggctggaa gcttctgttt
    41761 aacgggcacc aaagcgccgc caaaaatgga cagagttcgt acagcaccgc gacgatgaac
    41821 caggacatcg gctttgtcag catcgcaaac aacaagaccg ccgggaacat tgtctggctg
    41881 accaccaccc ccggaaatga agccaactca tcgatcgcgc gctggcagcc gatgggaaat
    41941 gctgccgaac agtatgtcgt cggctggagc agcgggacga catacaagtt gggccgcgtg
    42001 tcagataccg gcgcgttttt ggaaggtccg attgaccttg ccgcggccaa gtggggacag
    42061 cgcgacgatc cattccgcac ccacctaaac ggtgacatcg tgtgggcgtg gtttgatgct
    42121 gcgggttcca cccagctcaa gtttgctcgt ctgcgttcgg gcggcaccgc gacctgcacc
    42181 aagctgtagc gttgggagtc ctttcgtcca gtccggcgcg acagtccgct tccgcgccag
    42241 ttcctgtgat acgatcgctt gggtgaacga taggcgactc acttgcatcg taggtggttt
    42301 gcttttgtgc ctggtggcga tgtacttgtg gggcatctgg agcctggtct caccgcagct
    42361 ttgggcgctc cgctgggcac atcaaatgac gggcggctgg ggacgcgcga ttgggatggt
    42421 gtcggtacta aactttgcgc tgattgaagc gcttgtgttt gtgtccgacc cccgcaagaa
    42481 gctgcgcgcc atcgaaccag agatcgcttt tatcaagaag aaccacgccg ccgatcggca
    42541 aaagagagac attctgcttc tcgaactgta tcggcgagaa ggaatcgaag gctttgcgct
    42601 gtgggtcaag ccgctgatgg cgcttgtgac gatttggctt ctgctcgcga tgtatgtcgc
    42661 gctgtggaaa attccggagc tgcgcggtgc gtcgtttttt tggattccgg acttgagtgc
    42721 gcgcgatccg ctctatctgt tgccggttgg gagtgtgctg ctctctgtcc tgggatggct
    42781 ggctgcaccg aacactttgc cgccatcgga aagccgcaca cgcggacttg ttccgacgct
    42841 gctgtttgcc tgcgtggtgt ttctgtttcc cgcaagcttt gtgctttggg gcttcgcatc
    42901 caaggtgatt gtgctttccg gaaacgtggg caagcgaatc ctgattctgt ttcgcacctg
    42961 atgcggaagt ttgctgtgca tcggtgccag gatgcgattc ctatacgatg cgcagatagc
    43021 cggcgcggta gaagtcgtgg aaaagttcga ccgttggtcc gtcgcagaga agcggcagtg
    43081 gcaacgatcg atgctgcacg agctgacaaa atgtctgtag cgtctgtggc aagacgtgat
    43141 gcgcttctcc gtttaggaac agacagtcac cgcgcacaag accgcggctg attttggcaa
    43201 gctctaggtg tccctgtcct tgcatgcgtc ttcgaaacgc cgagagggaa agcgtccgtg
    43261 atggcgcagc aaacgattct ggtagcttgc ggcccgtcaa aagacgtccc aaaaagtccc
    43321 ctaccatgtc gcggcttacg cgcacctgcg aaagcacttc ggccactttg tcgaccatcg
    43381 catcgcttag cgccagcggg ttttcctgtg tccgcagatc cgggtcttca tagagcgcgt
    43441 cgggatcgat tccctcttcc agcacttcgt ttaggtagcc caggaagttt tgcacaagct
    43501 cacggtggga cggtgccaaa aaaccgatcg aataggtgaa gcaaggttcg tcggaaacgc
    43561 catagtgagc gaggccaggc ggcagataca gaatgtctcc aggttcaagc agccactctt
    43621 cctctggaac aaacgacttg agcactttga tggctgcttc ggaatccagt gtgcgatcgg
    43681 cctgcgtgct gacttgccag cggcgccgtc cggggccttg cagcaggaag acatcgtaca
    43741 gatcatcgtg tggtccgacg gtgccgccag ggcttgcata acttaccatc agatcgtcga
    43801 tgcgggcctg ggggacaaat gaaaagcggc gcagcagttc ccatccaccg ggtacatgac
    43861 tctcgatgcc gtggatgaga agggaccagt tgttcttggg tagggtgcgt ccgtccagtc
    43921 tgtcaaacgg accatcgtag cgttcccagc gaccgggctg tttgcctttg gtttgggggg
    43981 cgacaacgag ccgcgatgtg acgtctttgc gcgtggccag ctctaggaat gcatcacggg
    44041 cagcggtgct ggcagacttt ccggatgcat gtggctgaaa cagaccgcgg aagttgggaa
    44101 tcgcttgccg gatcagcagg ggccgcttct gccagtggtc gcgcaaaaac gtctcggcgg
    44161 tcatgtcgcc aagaacggaa gcttcgacgg gcgcggttgc ggggaatgga tccatatcgc
    44221 gggattcgct ggtcacgtcc atgagtccct gatttctaca tcatggcttg cgttttgggt
    44281 ggacttggac ggtttttcgc cgttttgggc cgttccgcgt ggtttgggtg cgaatttgcc
    44341 caacctgatg gccgcgtttg ttgcgatatg ctgcgcgatt gcgatgcgca tttttctcat
    44401 cacgcctccg atgacccaga tcaacacgcc gtatccagcg acggcgtact tgaccggctg
    44461 tctgcaccgg cacgcaccat ccgtcgaggt cgtgcagtcc gacttttcgc aagagctgtt
    44521 tttgcggttg ttttcgcagc gcggcttgca gcgggtgttc gacgagctat ccatgcgggc
    44581 tcgccgtctg cgtgggcgaa gcagtcggat cgacatgccg gattcgatcg cgcactttct
    44641 gaaacacggg gagacctgcg tggcgcttat cgagccggtg ctgcgctttc tgcaagggaa
    44701 agatccgagc ctggcgctgc ggattgtggg tcggacgctg ctgccggaag gtccgcgctt
    44761 tgcgaacgtg gaccatgtcg ctgggaatct cgatgggaaa gcgccgctcg atgctgcgtt
    44821 tggcaagctg ggcaccacag acagagcccg ccaccttgca agtctgtttg tagatgattt
    44881 ggccgatgtg ctgcgcgacg ggatcgatcc ccgcttcgag ctgtcacgtt acggcgagcg
    44941 gctggcttct agtgcgccat cgtttgatgc gctgtgggaa gcgctggaag acgagccgac
    45001 gctggtggat gaaacgctgg atgaactgac cagggaaaag ctgcgcgcgt ttccgcccga
    45061 cttggtgggc atcacagtgc cgtttcctgg caacgtctat ggtgcgctgc gcatcgcacg
    45121 gatggtcaag tcgatccatc ccaaagcccg cgtggtgctg gggggcggct atgtgaacac
    45181 cgagctgcgc cagctttccg atccgcgtgt gtttgacttc tgcgattaca tcacgctcga
    45241 cgatggggaa cggcctctgc ttgcgcttgt cgaacacttg cgtgatccca atcagccgct
    45301 tgtgcgcacc ttcgttcgcg aaggaaatcg ggtggtgcaa aagacgactc ctgcgctttt
    45361 ggatctgcac caccgcgaca ccggaacacc aacgtatgcg ggactggacc tgtcgcagta
    45421 tgtctctctg ttcgagatgc taaatcccat gcaccggctg tggtcagacg gacgctggaa
    45481 caaactgacg gtggccaagg ggtgctattg gaaaaaatgc acgttctgtg atctgtctct
    45541 ggattacatc gcgcgctatg aaacagcgac ggcagatgta ctggtagatc ggatcgaagc
    45601 actgatcaaa gagaccggac agacaggctt tcacttcgtg gatgaagcgg ctcctcccgc
    45661 ggcactgcgc gcactggccg agcgcttgat cgcgcgtcgg gtggcgatta cctggtgggg
    45721 caacattcgc tttgaaaaga gcttcaccaa ggagctcgtc gagctgcttg cacgatcggg
    45781 ctgtatcgca gtcagcggcg gactagaggt tgcttcggac cggctgctgg cgcttatgca
    45841 aaagggggtt acggtggcgc aggtggcgcg cgtgacccgt gcgtttagcg cggctggaat
    45901 tctggtccat gcctatctga tgtacggatt cccaacccag accgagcagg aaaccatcga
    45961 ctcgctggag cgggtgcgac agctctttgc cgaaggttgc atccagtcgg gcttttggca
    46021 ccgcttcgca gcgacggcgc acagtcccat cggacagaat ccggacgtgt atggaattcg
    46081 tcttttgcgc cagccgcggg ttcgctttgc gcgcaatgaa gttccgttcg ccgatccgac
    46141 cggctgcgat catgacaagc tcggaatcgg tctgcgcacg gcgatttaca acttcatgca
    46201 tggcattggg ctcgattccg atgtccggat ttggtttgat ccgccgtctg cttcgcgttc
    46261 ccgctggagc cggcgcggca tccgaggtgc aaacaccatg gcggtgccgg cccccgaagt
    46321 accgcccgac ttgattcgac aggcgctctg cgcaagtccg tgactgcatc catctgcgtg
    46381 atcgtgtgat actggctggg cgcgcttgcc tggccgccgg agcttgcgat tccttggcca
    46441 aaggcgggcg gttttccgta tcatcggtcc atgggttttg tcgtagagcc aattggtgtt
    46501 gtgcactgct ctcggaccgc tccggaagat gaccgctggg acagcgagac ctgtctgatc
    46561 aggctcgatc cttcacggct gagtcccgat gcggcgcttg ggctggatgg tttttcccat
    46621 gtcgaagtca tctatctgtt cgatcgggtg gatgaagcga aaatctgtcg cggtgcccgg
    46681 catccgcgcg gacggaaaga ctggccgctg accgggattt tggcacagcg cgccaaagat
    46741 cgtccgaacc gcttgggagt gactgtctgc cgactccttt cggtttccgg atttgacttg
    46801 tccgtcgctg gtcttgatgc gattgatggg acgccggttc ttgatgtgaa gccctatctg
    46861 gcagagtttg ggccgcgtgg agagcttcgc caaccagcgt ggtctacaga gcttatgcag
    46921 gactattggg agtccaagtc gaagccgtag tcgggcgggg aagccgtacg atctggcggg
    46981 tgatggtctg gaaatgatct gcggttacaa ccgggtccag gggtttatcc cttcacgccg
    47041 gagctgacgg tagatgagca ccatgtacca aagatcgaac acgaaacaga cggcgacgaa
    47101 tacgccgaca aatcgccagt tgtcgcggtg ctcgattccc aaataagaca gccccaccgt
    47161 catggatgtt cccagccact tcgtccatgc gatcgtgaat gactgaccct cgatcgatcg
    47221 ccgctgatag aacatcgcga caaacagcac agacatcgcc gcattttgga tgaatgcgat
    47281 gaactttccg ctctcgaacg gatcgccgcc gaagtacgga aggaagtcct tgaaaaaagg
    47341 tgcgccgagt atcatcacgc caaacgatgc caaaaacgcg aggaaggtga agacgtagaa
    47401 ctctgcacgg cttatcccat agtgccgttc aaagtacttg tagccgtaca ggaaaaacgt
    47461 cgccacgatg ccggcatcac acagcatcca caccgtgttg atgatggttc ccaccagctt
    47521 gatggagctt ggtggataaa tctgcgaata gacgatctcc caggtgaagt tgagcgccag
    47581 tgcgacgagc ggcattccat acgaacggtc gcggaagccg cggtacacaa gcgcaatgta
    47641 cgtgatggtc catgcgaggc cgctcagcac gacggcggtg aagttgagat ccatatcggt
    47701 gtccctccct ttttaatgtg tggtgatcat ttggtccgcc gttcgtcgat agcgcggctc
    47761 gattcgggcg tcttcggcgc ggtatcctag cgcaacgaca agcgttggtg cagtgccgcg
    47821 tggcagacac agtgcctgcg cgacctgtgc cccgttgaat ccttccattg ggcacgcatc
    47881 gacgccgtgt gcgcttgccg aaaacagcat ggtctgcgcg gccagcatcg acgcccgcgc
    47941 cgcccagttc ttggctcctt cttgtccaag cggcagcagg ctgcgacttg gctgcaacac
    48001 cgaaaggatg ctgcgcgcga caccaaggaa tggcagcagc cagggattga gtgcaaggga
    48061 cagacgatcg agtcccgaca ggtggtactg ccgcgacttt tcaggaagtg gcgcgctttg
    48121 gtagtacgtt cgcgattcct ctatgcgacg ccgtatgatg gacacaccca cgacaaacac
    48181 caccagcgct tgcgccgagc gggccgcgcg ctgaccgtta caggccagcg caagctgatc
    48241 ccgcagttct gggtttgtga tcaaatggac ttcgtagggt tgtattccca cgctcgatgg
    48301 tgcgtcgccc gcagcggcag cgatgcgtcg cagaagctcg gggtcaatcg gtcggtccga
    48361 aaagtctcgg accgttcggc gggtggaaat ctggtggtac acagaagtga tggaatcggc
    48421 ggttggcgtc gattcggaat gacttgggat ttgcatgact tcatgatagt aagtaattac
    48481 ttgctttact acttgcgttc caaaccggtg cagaggcatc caccatggcc aaggtcagca
    48541 cacgaaaaac gccacagcag gctcgctcaa aagagaccgt tgcggcgatt ttgcaagcat
    48601 ctacttacat tctgatagaa gacggctggc gcagcttcac caccaaccgc gttgccgagc
    48661 gtgcgggggt caacatcagc tctctgtatc agtattttcc aaacaaaacg gccattttgg
    48721 cggcgcttcg cgaggcacac gttgcggaca gccgcgcggc gctgatcaaa gccattgccg
    48781 acacccacga tcccctccgc aacatggtgc acgcgctgat cgattcccat cgcatcgcac
    48841 cgcagcttca tcgtgtgctg acagaagagc ttccttactc gctgcgcagg aatgtcgagt
    48901 gcatcgacca tccaattctg cacgcggtcg ttcgcaacat gttctctgct gcacccgatc
    48961 ctgacctggc gttctttgtt gcgcgctcgg cgctccatgc ggttgtgcac gacgccgcat
    49021 gccaacgtcc ggaactcctt tcctccgcag catttgtcca tgaagtcgag cgactcgctt
    49081 tgtcgctggt tcacagaacc tgagccgatc agccgtcgat cgaaccgaac atcacgacat
    49141 cctgtatagt gccggccatg cgacgcttgt ttctgttgct tgcgatcggt ggttgtgttt
    49201 ccggttcccc ttctgtcgac gcgggaactg atgcggaagt ggatcactct ccgctttccg
    49261 acatggccca ggcacccatg gacttggccc cgctgtgtgg cgcgccagca tgccgatccg
    49321 gtgacacctg ccagaccagt cagggctgct gttcctgtgg accgtttgca cgcgcctgtc
    49381 tgcctggctg gacctgcgct gtgccggcgg caaacgatgc gcgctgtcct gctgcggaac
    49441 cttcagcgct tgggtcttgt tcgttgccgg atggggtgtc ctgttggtac tgcgcaggtg
    49501 ccagtccgca tcgtgccgtc tgtgcgagcg cgaatttctg gtccgaatgc caaaagacag
    49561 gcgcgtcacg ctgctggaat ctggccggcc cgacggtgtc ttgcgattga caagtggtgg
    49621 gaacgttatc ggtttgcgga ctgtagcggc gtggcgacat caccgacatc gctgagcggg
    49681 aatccctgtc cgacggcaag ctgtagtgtt ttggcgagcc tctggctgtc ggcagacagt
    49741 cgcgtttccg tgctgcgcag tctgggaatc agcgcgtcga cgacctgtct gtcgcctgat
    49801 tcatccggat tcgatcccac atgatcgatg atcgttgaaa gagccgaaac ggaatcttcc
    49861 gacagctcgc gcgtgatccg cagtccttcc tggaaaaagg aaggcagaaa gcactccggg
    49921 tgaaagtgac gacagacttc gatgtcaaag cggagtccca tcacccggtt ggccagcgcg
    49981 tccgcaccaa agtttcgctc gcggaatgcg gccatcgtca tcgtgaacac gcgctctact
    50041 gccgcgcttg ccagtggata atcccactgc aggtaatcgc cgacggcgcg accttcctgc
    50101 tgcattcgcg acaggagcgg cgttcccgcg tacagctcga cccgtccgaa gttaaacggc
    50161 agcgtagatg ctctctgcat gaagcggaca ttttcacgca agctgtgcag cgttgtatcc
    50221 ggatcaaaca gcagcaagtt aaagcaggtg taaattccca gcgcttccac aagtgccagg
    50281 gcctcgtgat tctgtttcga gtgtgcccag cgacgcagcg tgcgcagtcc ttgttcggaa
    50341 tcactctcga cgccaacgta catgcgaatg cagtgtagcc gcgtctttag gatctcaaac
    50401 acctgcgggt cggcatcggt aggacgcgct tttaccacgg tggcgaaacg tccgatgccg
    50461 cgcgcctcga gtgcatcggc cagcgcagaa aaccgctcgg cattgcgctg atgccctgga
    50521 acgaaaaagt tgtcatcgtg aaagacaaaa atctcgatgc cgcgattcct tttttccgcc
    50581 accatttcgt cggcaatgtc ttcgacagaa cgcaagcgat agcgcgtacc gggaagactc
    50641 tgctggtgcc acgctgcgat acagcagaag ctacagttgg cgtagcagcc gcgacttcct
    50701 acgatgggcg caattccgtg tgcgaagcag cgagccggtg ttccccgacg atcgggccaa
    50761 ggcagcttgg ccaaatccgg aagcggcgga tgatccgtta gcaccacctg cccatcctca
    50821 ttgtggattc ccaaaccagc gacgtgcgcc agtggtgttc cgtcgcgcac cgcttccgtc
    50881 aggcgcaaaa atgtgatctc tgcttcctgc cgacagatcg aatcaatttc aggaaaatca
    50941 cgcagcagtt cctgacacgc gaacgtggca aagtggccgc cacaggtgat gtgtccttgg
    51001 tagccacggt ggcgcagggc aacgacgaga gccagcgcat ctttggctcg ccactgaaac
    51061 gcgagcgaca cacccacaac aagcggaggc gttgccgcat ctacaattgc agacaggacg
    51121 gatgcaaatg cccgctcgct gttaaaggga atgatctctg cacggatgcc cgcttgtgtc
    51181 agcaccgacg cgagatagcg caggctcagg ttttcttcca gctcgggacc aaccagcgcg
    51241 acccacgaca tggctagttc ccctctacct ggaccaccac cacatcgccg actttgggag
    51301 ccatcgacgg agtgatcgac aagtcgaacc agccacgcga tgcgccttgg caggtcgcac
    51361 ccagaaagac accgatgttg gtggtgccag tgggcagtag ccgcatcgtc agaacgccag
    51421 tcactggatc gacctgcgaa gacacgagtg tcgcgccgct tggtgaagag gaggaactgt
    51481 caaagcgaat ggaagacggc ccagcggttg gggtcagctt cacttccagc agcaagccca
    51541 tgggatcgct gacatactgt cctgttgcgg tgagtttgcg ctgttcgtcg acgcaacgcg
    51601 atggatcagg aagcgggtcc acgcatccga aaccagtaca gcccaacgtg taaagaccca
    51661 cgccgcccat catcagcttg ctcatgtgcg aaagaacgcg cgtccgcacg ccgcggccat
    51721 ctgaatgagg actgaccttt tcgggggtcg gcagcacttc ttccatgcgg gtcttgggcg
    51781 tctctgcctg gctcatctgc ggctccttgt ccgttgcggt gttttcacaa cagagcatga
    51841 ctgatttgtt tcgtacagca tatcgtgcat cggtgatatg gcgcattgcc aggacgattc
    51901 ctcttcccgt cgatgctgcg cggtatccat cgtgaagtgg gctcactgtg tgcggaacga
    51961 tgctgcggag agattgaaaa caggagccaa caagcgcttg cgcatgaact gtatcccatc
    52021 cgcccacgtc cccattcatg acgcgccaaa tcgacccgga gtatccccgt ttaaacccat
    52081 ccaaatgaac aaaatcaccg tcagaaaacg cctgtttgtt gatggcatcc tgaagtggaa
    52141 cgggcagcgg tttggggggg atgattctgg ggtgttggcg agcttgctgg ggggatgggc
    52201 gagtgggtca gacaacgtct ggattgtggg agagggcgga accctgctgc accagtcagg
    52261 agggggctgg cagcaggtgg atacgcagac atcgcgcacg ctgtcggcgg taacggggac
    52321 agcggacggc gtgtttgcgg tgggcggcag cggtagtgtg cttcgctggg acggatcgtc
    52381 gctgaccacc gaaagtacga cttgtgcaac ggcgcttctg ggaatcggtc aggtgggcgc
    52441 tgatctgctg agtgttggcg aaggatactg catgacgcgg cgcagcggcg gtgtttggac
    52501 tgctgttccg agcatgtctg tgcccccaac agacaagtct ggttacttct ctgttgttgg
    52561 ttcctatcaa ggcagcagct atgtgagcgg acgacccggt acgctgtaca agctggatgg
    52621 aacttcgtgg tcgcagcttg agctaactac gatgggtgtg gcgcggagtc tgcttgttct
    52681 tcctactggt gatgtctgga ttggcggaag tgatctgtat cgcgcaaatg gcagtcttcc
    52741 ggttcgactc aatgcgcgtc ctcaggagac gctgtatgca ctgtccggcg cgacagcgga
    52801 cgcgatgtgg gcaacgggtt ttgctggaac cgtactgcgg cgaaacggaa ccacatggga
    52861 gccggttcgg agtgatgcca cgtcgcctct gtatggggtg tatagtccgt cggctgatgt
    52921 ggcgttcgtg atcggatttg attcatctga aagtacgctg tcgcgctgta gcagcaaggc
    52981 ctgttcgatc atcgaaaaag catcaaacct tgaatacaca tccattcacg gaatcgatgc
    53041 cgacaactac tgggtggttg ggaagtcggg agtccttaag atgtggtcgg ccaagagcgc
    53101 tgccctcgtt gatgaaacag gcagtctgcc caagggggcg tcgctaaacc atgtccatgc
    53161 agttgctggc gcagggggag caagaccctc tgtaattgcg gttggtgagc agggactggt
    53221 catgcggcgc accgccatgg gctggctaca ggactcctca gctccaacgg gaaaacagaa
    53281 tctgctcgcg gtctgggcat ctgcggacag tgatgtgttt gtggggggaa gcaacggact
    53341 gattgctcac tttgatggat cggcatgggc tgtgattccg accagcg
//
LOCUS       DAORGN010000010        53017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_206_length_53017_cov_5.734867, whole genome shotgun sequence.
ACCESSION   DAORGN010000010 DAORGN010000000
VERSION     DAORGN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 53017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53017
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206_length_53017_cov_5.734867"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1907)
                     /locus_tag="PKL17_02235"
     CDS             complement(150..1907)
                     /locus_tag="PKL17_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNK43573.1"
                     /translation="MSAAPPNRPKSRLDLTLALLLLVATTGLLQLASRREGFARDEGY
                     YFHAAEQHAAYYEELATRLAHGDGKALFDRGLIDRFFSYNNEHPPLLKTLFGLSWRLL
                     HRCNCLSETGMHALPYVKRHRTLGLLKNGEAMRLPAHALAGSLVAVVFLLGRRLFGRA
                     GALAAAGLTILAPRHFFHAELSCFDAPVTAMLGLSTWAYLRAQQTRRLRWAVISAVLF
                     GLSLSTKHNAFFLPPLLLLHALWLRRDELRSGTGSKGQRWLRYLFQPWALAMATIGPL
                     VYFLSWPYLWFDTVRRFTFYVSFHLHHVHYNIEYLGQNFNRPPYPWHYVPVMTLLTMP
                     AVTLLLAKLGTIVWTWRSADVEAPIPDGEQRAGELPLLVLSAVWPLLIIMRPGTPIFG
                     AEKHWLPAVPFLALLAGAAVAYLIRQVSESMRVQRLLAPLVVAVVLLPAALSVYRSHP
                     YGLSHYSALAGGPVGAANLGMNRQFWGYSVRGVMPFLNQALLPHTPVYFHDANWFQLQ
                     MSQLDGFLRKDIADSGMEEPGVRSSRAALVMHEKHFNKYEYWIWDAYGTTKPSYVLTH
                     QGVPLCTVYQRPDPDRGGK"
     gene            complement(1904..2809)
                     /locus_tag="PKL17_02240"
     CDS             complement(1904..2809)
                     /locus_tag="PKL17_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43574.1"
                     /translation="MSRLFPRLGLAGVALVALWESWASLFPPRISDLDVRSAAQAVRA
                     AHKPGELIAVSPAWLSPLIQRELGDLMPVEHLGRADGKRFAGITEIFLSPHHAADVDG
                     LHSDGELRFGLLRVRHYTQTPATVSYDLSAHFLDATVSQIPLAGTAPESPCLWTGALP
                     TPLPAPGSLGGFVCSVGRVERRTMEIDYQPRRGIVSELAQGQKTLLRFSIPDADYSGS
                     TLHLWLGLHDYHQRKQAVGPVDVVVDVDDGSARHSLQVQVGQGFVLHKLPLPAKSGDV
                     HHLRIELSAASAPHHLLGLHGELRR"
     gene            complement(2816..4870)
                     /locus_tag="PKL17_02245"
     CDS             complement(2816..4870)
                     /locus_tag="PKL17_02245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNK43575.1"
                     /translation="MSKTRATLSAFSSLLLCSVFLPASTTSAQPKSPVGNASGIDESI
                     VDRAVNPCDDFYRFACGGWIKKTDIPADRPAWSRGFSEINERNQTVLRELLDENIAKP
                     GSDPNSKKLSDLYKACMDEPAIEKTAAADLQALLKPIGDVKTQADLAKELARQHVGIG
                     KPMFDFSASPDFKNANQVIGNLDQGGLGLPDRDYYLKTDEKSEALRKEYLAHVERMLV
                     LSGVSKESAAAQAQTIFAIEKRLAQASMSRVERREPKNLYHRIDIDGVLKLAPNFQWN
                     AYFAALGIPTVKEINVATLDYFKELNKLVVEEKLENIKTYLRWQQIHSSAMALSKPFV
                     DETFAFYSKRLTGTDKILPRWKRCVGFVDHAMGEALGTAFVSKTFGDDGKAKALEIIH
                     GIEGAMEQSVKALPWMDEPTRKAALEKLTRIANKIGFPDKPRSYDALKTQPGSHLANL
                     SAASAFEARRELAKIGKPRDKNEWLMTPPTVNAYYEPLFNEMAFPAGILQPPMFGRNV
                     ARAINYGAIGMVMGHEVTHGFDDEGRQFDADGNLRDWWSQSVNSEFNRRAQCVVDQYN
                     EYKPLPDANLDGKLTLGENIADLGGLKLALLAYRAAQKTNPVASKGSYSEEQLFFLGM
                     AQAWCEKRRPEYSRLLINVDPHSPAEFRVNGPLSNLPDFASAWKCAPTAKMVRKNQCQ
                     VW"
     gene            complement(4924..5865)
                     /locus_tag="PKL17_02250"
     CDS             complement(4924..5865)
                     /locus_tag="PKL17_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNK43576.1"
                     /translation="MRILRRIGPLILLLLLLFWALLRLDLRAFAQSIGRSHISLWVLA
                     ALLNVAIRAGARAERTRVLLRSLAGQAPHFLGLYVDLVSAQAATVLWPPAGMGFRLLV
                     LRRRYGFGGVELVGEQLVEKLLDGMSLGLWGFLALLTGELPAAIRLAVIVACVLGVAA
                     SLGFFLSDLPFLRRRLPPMPKISGPSLLWTAVLDGFDMVMLHLCLQAAGVSVSLHVLL
                     LLFVALNLAVLLPLTPAGFGVVEATVMLVLRPLGVPAETALAGALLYHLAVVGPFLLL
                     GPLASVRQVFAEARAAQAPAVAVEHKAEPTSHLRPDS"
     gene            complement(5928..8336)
                     /locus_tag="PKL17_02255"
     CDS             complement(5928..8336)
                     /locus_tag="PKL17_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNK43577.1"
                     /translation="MRRLLCDAGYELWLSPAGFELFASRSLLSDQADSATETAEKQSL
                     LGAFQVTAEGPGGRALLVTSEAALYTLVDGPDGSIELRFPAADGSGLLWIVHARLDHR
                     GLLLWQSLRNQSGEPIRLSAFRLTALGLQTSFVAGLGEQPGFQVFRTGYSSFTPSFSV
                     DSRFREEKPLFPTAASFNLLTESDYYGKPGCLSSPWMMVLTPPAQPLGHLLVGFVSAL
                     CGTGEVAICRDGAPRIEARLGFEGRQVAVGETLTGDKLLIALSHSEQGILDEYVDETA
                     RQMQPRPSPSPVPSGWCSWYYYYTKVSERDVLRNLTALAEKKLPVKYVQLDDGYQRKI
                     GDWLTTNAKFPSGLGGLARQIKEAGFVPGIWLAPFFVQRSSEIFQHHRDWLLRDEDGK
                     LVRLGYHPFWGIPDGQVYSLDLTHPAVLAWLRRVFSTLCELGFEYFKIDFLFAGLRRG
                     LRHNPQLSAVEAYRQGLRVIRETIGDRFLLGCGAPLVPSIGFVDAMRISPDVKEDWRD
                     DLVDLIAHGTGYPSAEMALHNCLTRAHLHGKWWCNDPDCLLVRRDKSSLSLPEVQTLL
                     SVMSLTGGMLLCSDDLRVLPGERVQLLSRTLPPSGRTARALGVLREEKPQRFVFVGDE
                     PSGDRAALAALINWHDSGREVVLSAQAIGLPEADYVAFELWSRTMAQLDPLQTLTLRL
                     APHGTALWLLRRRKPHPQVVSVTHHVWQTLQLLQSETWDADGNRLTVTIAGCTRGDGE
                     IRISVPDGLRLQKVERLSGPIELRGDALVSAGIALACELDGSNRPSAIAVYFEPRGCI
                     QDTR"
     gene            complement(8339..9655)
                     /locus_tag="PKL17_02260"
     CDS             complement(8339..9655)
                     /locus_tag="PKL17_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43578.1"
                     /translation="MTQILFVLIVGLLFADQNLLAPNLTAIGNEFGFSRAEIDQRLGA
                     DVNLLFWMLGGVLTLIVGYLADRGDLADKLSRKWLLTIVAVIGQLACLGSGLAKTYDQ
                     LLWARALTGIGIGGAFPLIYSLIGDYYPPHKRASATATLGLSQGLGIGLGQLLSGMLG
                     AQSGWRMPFYVVALPGLALSVLFALFAKEPRRGQHEEGLHDLLASGKAYEERLRLRDL
                     PQLFAVKTNLLILLQALPGTVPWGVFFVYLNDYYAHDKGFSVPEATLLVMSVGIAAIV
                     GGFIGGLAGQKLLNRKARNLPLLCAVTTILAVIPMAVLIEYPVRPGQSLLGPLIVGLL
                     TGSLAAVTGPNVNTMLIAVNPPERRGSAFSFFNLCNDLGRGLGAWIVGGMAARLGRVS
                     AFHIANLMWLGCGVVLLCLVFLFPKDERALQDRLRALSLRKLGRGE"
     gene            9905..11125
                     /locus_tag="PKL17_02265"
     CDS             9905..11125
                     /locus_tag="PKL17_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43579.1"
                     /translation="MKPPDLCRRLLPWIVSGLCGCLHFQPIDRPLGDDLNERAALIDR
                     AQHGAMGQRQQVLVAKALMATHGEELSRLKSLIDRGTDGATLHRLIYHDVSDPVLRQA
                     LVAHLAREARSTDALTAHVPRDKVRVLSDIDDTLVRSLCDERYEKHTLLPGVLEFYRA
                     LSVGARAREVEAQAPGEVTFLSARPKELSGLSYSSLLNPLLSARGQIIAGYNLLPAPA
                     AVPGIGNVHEVQAFAKFRNFVELATLYPESDFVFIGDSGQGDMLGGELMLYHQPERMR
                     AVFIHQIITDGKHGILCPYPRSQSDLDRGTSRVVFFDTYIGAAAQAHEWGLISEDSLY
                     VVTKSAIETLNQRGSEILTAKLRNLVIAQFRRDIRRVQEILANHPVDTARDRLPDLLL
                     AESSCEKNPATKNP"
     gene            11214..11996
                     /gene="larB"
                     /locus_tag="PKL17_02270"
     CDS             11214..11996
                     /gene="larB"
                     /locus_tag="PKL17_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNK43580.1"
                     /translation="MNEAALQDLLSQVATGQLEVQAAMQRLAKLPFADLGFARVDHHR
                     SLRAGFPEVVFCQGKRPEHVVAIFRELLSHGSAALGTRASIEHAELCQRELPQVEYHP
                     SARLLTSPRPSLPGPPLGPVLVVCAGTSDLPVADEAAITAEFLGCHVERLCDVGVAGI
                     HRLLAAEDKLRAARLIIAVAGMEGALPSAVSGLSGKPVIAVPTSIGYGASFSGLAALL
                     GMLSSCASGVTVVNIDNGFGAGYAAALTLRSATIPLTETPRS"
     gene            12002..13420
                     /gene="larC"
                     /locus_tag="PKL17_02275"
     CDS             12002..13420
                     /gene="larC"
                     /locus_tag="PKL17_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNK43581.1"
                     /translation="MTATCPSPTSPDFGDPSSLRGCTLYFDCATGLAGDMTLAALLDL
                     GVPESFVREQLSLLPLSHWELQVSSVRKHGIVGKKVDVIDLGAPLTELSHEPKHHGHD
                     HAHSHPHDHPHSDHDHDDPHHDAHGDDHDDDHHHHDHPHTHYADIRKMISSSGVLADV
                     KQRALAMFDKLAAVEAALHGVTIPEVMFHEVGALDSIVDIVGVAAALSWISPKRVVSR
                     TVPLGSGRVKTAHGLLPIPAPATAALLRGAQVEAGDLLGELTTPTGALIVSSYAAGFG
                     PLPPMQVLATGHGAGTRELPDRPNILRIIAGMEPQATTAPTHTDDTALWEICANLDDG
                     NPQLLPPLVDSLLSVGANDAWLDQVIMKKGRPGTVLHVLCDEQQRAEIVQLILRETTT
                     IGLRFHRVERTILQRSIVSVDTAFGPVDVKIGRDPHSQQILNVAPEFESVRKLAARCN
                     LPIKQVYSAAIAAAEKQRPTTN"
     gene            complement(13410..13592)
                     /locus_tag="PKL17_02280"
     CDS             complement(13410..13592)
                     /locus_tag="PKL17_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43582.1"
                     /translation="MLEPWIIEQIRKREQEQQRRHERQPLVPIPDYPPPRDSSDEDRD
                     DHDIPSGDRGVVIINL"
     gene            13752..14477
                     /locus_tag="PKL17_02285"
     CDS             13752..14477
                     /locus_tag="PKL17_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK43583.1"
                     /translation="MSGAPVEIIALRKTFAFGGRPIEVLKGIDLTIDAGEMVAVVGAS
                     GAGKSTFLHVLGTLDVASSGKVIIDGADVTSMPPTVLANFRNHTIGFVFQFHHLLPEF
                     SAVENAAMPGLIAGLDRRVAQSRAEELLKRVGLGHRLAHRPGELSGGEQQRVALARAL
                     VMQPRLLLADEPTGNLDARTGAAIHELVVELNRERGMTMIVVTHNEDLAARMPRRLHM
                     ADGRILTDEEAKRHATAVQEGVA"
     gene            14474..16909
                     /gene="bamA"
                     /locus_tag="PKL17_02290"
     CDS             14474..16909
                     /gene="bamA"
                     /locus_tag="PKL17_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNK43584.1"
                     /translation="MKRSFWILLAVWLGFTLPAQSASADVELPIGRRVEKIGFKGNIK
                     VEAAAMQRALSTEERFGKHPVEEGQPLKLDTLQDDLKTLWKMRSFDDVRIEWEEGDSG
                     GIVLQYVVREKRTVRKIYVSGNSEVGLDKINEVLDLKKDTIYDPAKIKRNVEKIHDLY
                     VEKGFYLADVKSDIKIHSDRQLDVYFIVDEHAKVEVRKVTFQGNRNATDEELRSVMAT
                     QEGNWLSFLTSAGTYKEDAFERDVLLLTAFYYDKGYVTVKIGKPTVEMSPDKRFLYIS
                     IPVEEGEIYKLGKLDFQGELLVPKEDMIAGLKVKSGDQFNRSKLGQDIQNINNFYKNR
                     GYANVNVTPLTQIDADKRIVDLTFDLQQGKKVFFERINIRGNTKTRDKVIRRELKIVE
                     GELYSEALVEFSKNRVQALGFFEKVEISTKRGTTDERIEVNIEVTERPTGTFQVGAGF
                     SSIENFIAQAQIAQNNLFGRGTTLRLSAQVSSLRQLFSINYIDPYFLDSNVTFSFSGY
                     NQLLYYPNFNRTTRGGDLTWGYLFGDYLRLLGTYKLEWVSIGQNTQGMQISGFGQVQS
                     IAGGTLANLYRRGWTSSVRLWTQFDSRNDRMFPTKGMFHLLSLEVADRYLASEIDYVR
                     VSGYARFYRPIWGPFIFRLNAESGLVFSRRAEGVPIYERYFLGGIQSIRGFRLFSLGP
                     RIQIQSALEPGSYLIPFSVGANWQIIMNSEIEFMIIPAVQIKGVVFFDMGNGYNLESK
                     YCTSAAATSALPEVFNPCSHYPTFQNLRYSVGFGFRWFSPIGPLRFEWGIPLNKQTGE
                     DPLVFEFTIGNFF"
     gene            16985..17557
                     /locus_tag="PKL17_02295"
     CDS             16985..17557
                     /locus_tag="PKL17_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNK43585.1"
                     /translation="MFNPTVRWTLGLGLALSVVLPAATAKAQQKIAIVDIRRAIEDSN
                     EGKANIGGLKAEADRKQKELLQKQDELKKLEEDFLKQQSVLQPAAAQKKREELQQKVM
                     QLQDALQRAQQDIGQKEAKVLQPLQDKVLRAIDALARRDNYNMVLRSDVVLWPQQSPM
                     DITNEVIRKVNDMKAPTPAPAGGAPTGGSK"
     gene            17560..18048
                     /gene="fabZ"
                     /locus_tag="PKL17_02300"
     CDS             17560..18048
                     /gene="fabZ"
                     /locus_tag="PKL17_02300"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNK43586.1"
                     /translation="MADSPALPLDVTAIMRLLPHRYPFLLVDRVTEFAGETCRAIKNV
                     TINEPFFVGHFPGHPVMPGVLILEAMAQTAAVHACLKLGKQAGEQAFYLVSMDKARFR
                     KPVVPGDQLILTTTLLRGRGRIWKFRGEVHVGGVLVSEAEFMATLPSALAQDEASGDS
                     ES"
     gene            18095..19531
                     /locus_tag="PKL17_02305"
     CDS             18095..19531
                     /locus_tag="PKL17_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43587.1"
                     /translation="MSRHTLLFLAAMGLCACNPRKPLPVSKDMGPAVQVVDAAPRPYA
                     GTPATKAAPPLAETEPNDDREHAQRIDPQKVIQGSLLPPTTSGAGKGDDDYFLWPAQP
                     AAQTLRIEASGAPELSLEVLTETATLAVIDERGAGEGERLAGLSIRAGQSVWIRVRGR
                     AKAEAATTSALGEYQLAVTATPASPDSEAEPNDTATDATPILGNDASGALSFRRDEDY
                     FVLPLPTLPGRKVTAFPNAEGLREPAILRVELTSPSILPALRVLLEPALEPGRDGGVA
                     APRLLVDVSAGKGKEELRLRNLTLPAGTGRVFIGVRGLAFAKPPGESRYHLRALVETP
                     LEGAETEPNDSCAASASPLTLASGTADIAGFLWPGDVDCYRVTGAAAQETTYEAKLSL
                     PGGDCSAVLEWVSADGKPVGAKSVGKPSQSAKAADGKNTDGKNTELTITTGGDFFLRV
                     LSRDRRSCFEAPYRLGVTSKEPGGGDKP"
     gene            19528..20199
                     /locus_tag="PKL17_02310"
     CDS             19528..20199
                     /locus_tag="PKL17_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43588.1"
                     /translation="MTRRIVLCLLLTACRTGSDPKQSLESYLHDTSRGSYDRAYDRLS
                     LDFRRRCDKPCFVRMLEAQPGASRQLLDELRSGTQSVVYQAELKLRDGTQLALEQQPP
                     DTESARRATPPPFLFAESPLDFYPQDSPKNALRSFVRAFAARRFDVLMRFVPKSLESK
                     LTADALRTRFDSEPRIASQVDALRQHLDDKVTVDGNTARLVTGPEQEATLMFEDGRWR
                     VQKLE"
     gene            20254..21339
                     /locus_tag="PKL17_02315"
     CDS             20254..21339
                     /locus_tag="PKL17_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNK43589.1"
                     /translation="MTLTSPTVPLVFRYGLHRVRDAVRVLPQAAWSVESQPDLCGGRD
                     YELLIDVERLNIDAASFRQMEEAARSVGEDIEAGVRRQILDTVHKRGKMHNPMTGSGG
                     MLLGRVRHVGGKLPDPHASLRPGERVATLVSLSLTPLQIERILSVNLHAHQVSILGTA
                     ILFDSGSWARLPPSLPESVALSALDVAGAAPQVTRLCHDPRPGKRVERVLVLGACGKS
                     GLLVGAAARKAGVKTIIGVESYAKAASEAESLGIYDQIVRKDAADALEIATEVCAGGE
                     FDLVISCVSAPRAEMAAIMCCRQGGTVYFFSMATSFTAAALGAEGISRDVDMLIGNGY
                     CIGHAGETLELLGSHAPLRELFIRRYS"
     gene            complement(21330..22388)
                     /gene="hemE"
                     /locus_tag="PKL17_02320"
     CDS             complement(21330..22388)
                     /gene="hemE"
                     /locus_tag="PKL17_02320"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNK43590.1"
                     /translation="MATFACTRKNRFLAACAGDPVDTVPIWLMRQAGRYLPEYQATRR
                     KAGDFLALCTTPDLAVEVTLQPVHRLGVDAAILFSDILLPLRAMGMPLTFGDEGPRLQ
                     PLADAAAIDALRVLSPDVLPEVLETVRILRRELSDVVPLIGFAGAPFTLLTYAVEGQT
                     GKTFQKTKRLLYCEPALAHRLLNKLTESLIAHLTGQVLAGAQAVQLFDSWIGVLSTED
                     YAEFGAPYVEKILTALRPLGVPLIYFPLGGLALLPQVGKLSADVVSIDWRQPLSVAKA
                     ALSSNQGLQGNLEPLLLLGPLAQLESRVKQVLREAPRRRYIFNLGHGLTPEIPPAHVE
                     ALVEMVHRLGAQPATDQE"
     gene            22644..24134
                     /locus_tag="PKL17_02325"
     CDS             22644..24134
                     /locus_tag="PKL17_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK43591.1"
                     /translation="MSGLTSYFASTLSSYDQILITAYLLVFVGLAVYGFHRSQLVYLY
                     YRYRDQRPEPLDRLRDLPMVTVQLPLFNEMYVAQRLLDAVAQIRYPRDRFEIQVLDDS
                     TDETQELCKRKVAEIAAQGIQISYVHRVDRTGFKAGALENGLKTAKGEFLLVFDADFI
                     PEPDVLERTIHFFSDPKIAMVQVRWDHLNRDYSALTEVQALMLDGHFVIEHTARHRSG
                     RFFNFNGTGGIWRRAAISDAGGWQHDTLTEDMDLSYRAQLRGWRFVYLPDVVSPAELP
                     VEMNSFKSQQFRWAKGSIQVAKKLLPTIMRSDVPLSVKTEAFFHLTNNFAYPLLLLLS
                     LLLLPNLLVRTTHGLREVLLIDLPLFFGTTLSIAAFYITSQREIDRGGWMRTIKRLPL
                     MMSLGIGLCINQTRAVVEALLQRDSGEFVRTPKHGVVAKIEAWRSKKYRADKTLIPAL
                     EVGFAAYFALAIVLAMKNGHWMSLPFLLLFFLGYAYVGLLSLVQLR"
     gene            24229..24987
                     /locus_tag="PKL17_02330"
     CDS             24229..24987
                     /locus_tag="PKL17_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNK43592.1"
                     /translation="MKLLVLAAGYATRLRPLTDHQAKPLLPVAGRPMIDHVLDKFAAC
                     PEIDGIYVVTNRRYAPNFVRWAEDLGGQFAGKPIHIVDDGTTSNDDRLGAIGDIGYVL
                     RTCNIDDDLVVVAGDNLFSGTLEDFVKTAKQRGILVGVYDVGDLEQIRKYNNLGLDDE
                     GRITHFEEKPQTPVSTVTAIALYHYPRAVLPWLHRYLAEGKNPDQPGRLVEWLFPQIP
                     CYTYRITGIWLDIGSRETYDEAQRLFQTPQGSKA"
     gene            24990..25832
                     /locus_tag="PKL17_02335"
     CDS             24990..25832
                     /locus_tag="PKL17_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43593.1"
                     /translation="MTRQTSTLLFLSLSLPLWLAGCALSENGPETSPPDLTPPPQRGI
                     VRIGSASVMSGPDTYEQSMALALFYDPNAASPVCTRSETRNCVTHRCGAIKNVPSAGD
                     ITIKGGSEDVLLRPGTSGAYTEYRNTSTLTFQRGQILNLSASGKDVPAWQTPLTIPTQ
                     VFSLQSPSPARIDLTWVLHKTQDLALTWTPTTPGHSVRIELSQDFGQSQGTLIECVFD
                     GSQGAGSVPASVIAQLQTTVGINHAVGVLVGPGSEIRLSPPGWDVSVWTIGVGRTGIA
                     TIAD"
     gene            complement(25890..27743)
                     /locus_tag="PKL17_02340"
     CDS             complement(25890..27743)
                     /locus_tag="PKL17_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNK43594.1"
                     /translation="MAQGQNHYLADLREIQFVLFEQCGLGELLKSAPFVGTFDDSGVD
                     MVLDEAYQFATTVVGPLNSAADVEGCRLVDGKVKTPAGFQDAWKRVYDSGLRLVSIHP
                     EWGGSGAPTLLGMAVEELVAGANTSFAMYPGLSIGVAEVIEKFGTQEQKALYLPRLYG
                     GKWAGTMCLTEPQAGSDVGSARTKAVKQPDGRYLISGTKIFISSGDHDLTENIIHLVL
                     ARTENAPAGTKGLSLFIVPKVRVNPDGTLGESNDVAVGGLEHKMGIRASATAVLNFGE
                     NSACLGEMVGGPASEHQGMKQMFNMMNFARIGVGIQSLAVASTAYLNALRYTRERKQG
                     SSIQNFKDPNAPRVPIIEHADVRRMLLDMKSRVEGLRALIAKVAAHNDRATIAAHGGG
                     DGKASYHQGQVELLTPLVKAYGSDQAFRICETAIQTLGGVGYTSDYGIEQYCRDAKIF
                     SIYEGTNHIQAMDLVGRKLGMAGGANLQAYLADIQAFVGSHSEHPNVGPEVKLLGEAT
                     AALQGTTMRLLSWFYGGQLELVPLVANRFLEMMSENTLAWLLLDQAVLAESKLAGLEK
                     DHPDQAFYVGKRHAAQYFARNVLPGVIDKADILAREDKSPVEIPDAAFARI"
     gene            27920..28780
                     /locus_tag="PKL17_02345"
     CDS             27920..28780
                     /locus_tag="PKL17_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNK43595.1"
                     /translation="MTTAASPRRPPLRSSHTPIGAQPVLDRPPSELTISEVADRSGVP
                     IATIKFYIREGLLPRPRTAGRTVGRYDSAFVNRLELVRELRNRWRLSLREIAELLQEA
                     GPLATLNEIELQLAARDRLDEAIDPTNAEPPISELRLVERSGLPMADIHDLTRYGLLT
                     PIKVDGATVYAERDARIAEVVGALRSSGYNDHLGFGVADLQRYVEALRVLVVREVEQF
                     DSPALRGLGRDEMLRLIGQGVAQVDVLLGLLHKKLLLAAVHDRLDAAASRRQTEGKPQ
                     KPARRSKAPR"
     gene            28930..30519
                     /locus_tag="PKL17_02350"
     CDS             28930..30519
                     /locus_tag="PKL17_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43596.1"
                     /translation="MLLDAQPAQKTPPTEELAAQLGKNYHGQVTLRRTVLGLFVALMA
                     IPAPALAYPYYSLRPVDFVLAGPTDGHLASLFFNPAAIRVQPGSQVLVQGGVSGFIGS
                     YQRQAALPSGFSPGGTGSPAQTVPIRWANPQALAALAWDLRSDSVTLGLAFSTPVLDL
                     TNFRRDDAPIEQLSTRYHAIYEESYSLWGSLAIGLKLKSWLLLGASFNFGYTHSSLSF
                     LRDGDAKARDSLGCGGGPCEQWASRTNIEMDVGALGYGFSAGAIVEPLLDRLWLGLSY
                     LSPLFSSQGDFVALEGQPSALSWDDGSGCSGSWKGAKITVGNQASQCGAARLMVAYPH
                     IIYFGGRLRFPLSPAVEKSADSVRPRAVELSSWARLTIPGHTDQEIQVERRLLEPGLY
                     TRPLSAQTAFAVTLGIRQYFQRVTLGQELLYETPRAAAGTVSPANIEGHKLDLSLSLR
                     AVLHKRLSLLLSVGATWMFFAPETGQSYDPSLVELCRQSGYDVTSSACDAVQHGLAIP
                     TAAASYTLGIPHGSAGLEINL"
     gene            30648..31925
                     /locus_tag="PKL17_02355"
     CDS             30648..31925
                     /locus_tag="PKL17_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNK43597.1"
                     /translation="MANCTIDTAPICDAASLMCRACTMGADDIACKNGRPSTPRCGPQ
                     GSCVACLNHGDCATKDLRKPACHNFACGPCKSATECQSKLCSADGSCAPVAEVLYVDN
                     KNGSCTGIDHKGSIDDPFCTLRDAVTAATMGGKTLISVAPSSKPYAALSVSPADQLTS
                     LQIVGSGTLPSETRIEGLLNESAIAVMGDGTMGKPTVQLRNLELRGGSSASASGVSCT
                     QGGAVTMSNIRVRGSGGSGISASNCNIIADQSQVFDNRASGISLTSSTFALSNLMLWS
                     NSVSGISLGVGNSGTMRFLTLSVNGNPASAQPAGIDCGATAHLVEHSIVFDNYSRASG
                     INSTDNQLVGCMLTNVVTNDKQATGGIYKTALDFVNPVADVSNIDLHLVKDSPKNRDA
                     CVDKVAQPPSDHDIDGTARPQGSAADIGAHEVQ"
     gene            complement(31983..32054)
                     /locus_tag="PKL17_02360"
     tRNA            complement(31983..32054)
                     /locus_tag="PKL17_02360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32020..32022),aa:Gly,seq:ccc)
     gene            32191..32859
                     /locus_tag="PKL17_02365"
     CDS             32191..32859
                     /locus_tag="PKL17_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43598.1"
                     /translation="MAESRSPRAPVRKSGTMRIETVGRDRSVYRTTALVVAVALGGAA
                     VGSLGSQLLARLTLRKAGPATTSKAPLLLLRSETLEGRLAEIDQALAQHDSSMALLLC
                     EEALRQNPSDPQLLLRRKRAEEEQLDRFRFEMLDQAISRRNFAAAVALAEEISAGSPL
                     RAQAQTRIQSVVAPYVKSLLDEAERAVKLSLCSEARTILSQVLQLDPQNSRAQEILSA
                     CPTN"
     gene            33047..34279
                     /locus_tag="PKL17_02370"
     CDS             33047..34279
                     /locus_tag="PKL17_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNK43599.1"
                     /translation="MSSAFANVNRYFTKAAQVLNLSAETAEQLMTPFRELRVECSIRM
                     DDGKIRTFVGYRVQHDNSRGPFKGGLRYSPDVDIDEVRALASLMTWKTAVVGIPFGGA
                     KGGICVDPSQLSPGELERLTRRFVQGIHLVIGDNVDIPAPDVNTNAQVMAWFMDEYNK
                     FHGFHPGVVTGKPVDMFGSLGRDEATGRGVLIATEEYLRTQDKKISDATFVVQGFGNV
                     GSHSARLIHERGGKVVAIGDHTASYYDPEGIHIPAALAYVKEKKVLAGWTGAPSIPRD
                     EILFHECDVLIPAALGGVITGENAGRIKCRVIIEGANGPTTPEADDILHARGVVIVPD
                     IYANAGGVTVSYFEWVQNIQQFYWEELRVRTELEKIMRRAFQELYATAQSHRIDLRTA
                     AFVVAIQKVAKATAQRGI"
     gene            complement(34308..34784)
                     /locus_tag="PKL17_02375"
     CDS             complement(34308..34784)
                     /locus_tag="PKL17_02375"
                     /inference="COORDINATES: protein motif:HMM:NF028004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HNK43600.1"
                     /translation="MNANMGMALCVLVAMSGCGSSTFEAQDADFVGFTSWAQTTAPRQ
                     GGGPSASVLGQAHSATDATITRSMYINNNANRGSDGQFPIGTILVKAHTKGGAMIGGT
                     AMAKRGGDFNPGAKGWEWFVLSASGTIMVRGGADVMGGICNGCHTAAQNTDFVFTR"
     gene            35047..35691
                     /locus_tag="PKL17_02380"
     CDS             35047..35691
                     /locus_tag="PKL17_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK43601.1"
                     /translation="MAVDPSRQQRSAPADEELIPRLCQGDAEAYVLFVRRYHGRLMRL
                     ARSFVASQASAEEVVQETWLAVLQGIERFEHRSSVKAWLFRILINRAKTRGQREGRTR
                     LFADLTIELPQEPDELLAQFAPNGHWLSPPAEWDHSTPETQLMSQETKRVLEQAVRDL
                     PPTPRAVILLRDIEGLDAEEVCQQLSLSEANQRVILHRARTAIRLAITKHMQKS"
     gene            complement(35695..35943)
                     /locus_tag="PKL17_02385"
     CDS             complement(35695..35943)
                     /locus_tag="PKL17_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNK43602.1"
                     /translation="MMKRVMWSCRDLTSVATDYLEGRLTFGQRLQFWLHLGICLGCRR
                     YLQQMRTTIALLGQLSEEPLPPEIESGLLKQFHSVQKH"
     gene            36104..36313
                     /locus_tag="PKL17_02390"
     CDS             36104..36313
                     /locus_tag="PKL17_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43603.1"
                     /translation="MNKKVLVAVIGSLSIAAPALSHADDAKPAKAEKAPKKGDKKGHD
                     TKGGEKSCGGDKSCGGGKSCGGKGK"
     gene            36401..36982
                     /locus_tag="PKL17_02395"
     CDS             36401..36982
                     /locus_tag="PKL17_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNK43604.1"
                     /translation="MRIRSQFIGTALLALSVLFPHQAEAKKFELDGNHSFFAFTASTV
                     IFDVEGKFTKYKLEASGDPDTGADGKIRLEIDVKSIDTGNKTRDKHLLSDDFFASAKF
                     PKLVFTSKKVAIEGDKLVVDGTLDLHGVQKDLKLTFAKSSGVNGAGVKEWAYKAELPI
                     NRKDFGIGADSVAAKISLKDQVTLRILVAGFPE"
     gene            37082..37474
                     /locus_tag="PKL17_02400"
     CDS             37082..37474
                     /locus_tag="PKL17_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43605.1"
                     /translation="MRRDIGAHQIEVDGDLVIARSYGRWTLQETEQMIAIWNELLEKY
                     GRLFEVGDFSDGIELDAAVRRLLIDWSKTHQFTALAIVVKSLPVRAIATLVVRGAQLV
                     NPGAPSPLFCSTFEEGYAFVEQARKQRS"
     gene            complement(37543..38043)
                     /locus_tag="PKL17_02405"
     CDS             complement(37543..38043)
                     /locus_tag="PKL17_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43606.1"
                     /translation="MRALVALPLSLLLLAGACAPKDDPVPEEQMMVPKVETKFVIRFK
                     LSDFVRGQIPKDSTVKGTVYGDLFLSEDVAITGPRKGAMAAASVELPIDLKTDKLSTA
                     SITTKPLAAQTYTFLGFFDIGDKSTPASRDPVTGDPVTLPFTNKFTLADGQTMPISVT
                     FDLLYN"
     gene            complement(38043..38975)
                     /locus_tag="PKL17_02410"
     CDS             complement(38043..38975)
                     /locus_tag="PKL17_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43607.1"
                     /translation="MTRLHSLLCLSSLAVFSLACSPKETEEEMLERTAVDVRVPMPAA
                     DPKFIDFVGAEKIIKSGEDSMTCTHLLYQGEDLAYDLADTLQGKFGHHFVILKAKNPL
                     PPGTSEDCSKPEDMAKFDILTMPSFELSANQAFMMRKGTPIVMQSHYVNTGKDPIRTR
                     DLLRFRRVDAAKVTTWAAAAGSYLEKFEIKPGEQATRSYDCVVPRDYKSILFGGHMHE
                     WGKKFVLSYAATPADTMKELYRVDEWIAHYRDDPPTLLMTKNPMDLPKGSVLRVECTW
                     FNDTKESLSHPHEMCTTFGVLLGSQEPWLCKGDK"
     gene            complement(39006..39422)
                     /locus_tag="PKL17_02415"
     CDS             complement(39006..39422)
                     /locus_tag="PKL17_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43608.1"
                     /translation="MTQRRAAGSVGFAFGLLSGVVGSVLLLSGCSPEPSLGQIERDVF
                     QRSCSFATCHQSTGTPAGGLSLGASTYDQLVNAKSTLSPGQMRVVPGDPDASFLMEKL
                     TSDKPKFGQRMPPAQALEPEEIEKMRAWILAGAKNN"
     gene            complement(39571..41064)
                     /locus_tag="PKL17_02420"
     CDS             complement(39571..41064)
                     /locus_tag="PKL17_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNK43609.1"
                     /translation="MQTDLWLMTLIWLVPLVASLVGRVFSSDGALRKLGIFTSGLVVL
                     LSLWAFARDASDHAFARTEAAWAPVFLGIRYHVGIDGLSAVLVPVSAGIVLAVLCAAP
                     RRLLTASLIREVLLTESLLLGVLISLDLSILTLFWVTSLVPLLWSLHKRGERGAVRML
                     TILGVCSSLPMVVFVVMLGVLRAKSGAAVQTPYDLLALGQRQMSEPLPNWLGILALCG
                     ALVRMGCFPFHVWIVPLSERGPAPLTLAAFATPLGMFLTTRVTMPLFPELFAQAMPLL
                     LPLGLLTASYGAVLALAQTDLRRMLGYFWISQQGFLLAGIASVNAPGVSGALIHAIGT
                     VVVRTGLALLIGSIVARAGTADIRKLGGLVRTAPRMATGFLLLAVAAVGSPGTLGFVS
                     EDLIVQGLLDHHGIAAVMVLLTTALNGIVLFLAFQRVFLGEATGEVASGGGFPDLLPR
                     ERWVAVSLFGLLLAGGLLPAPLLAVRTSVVEALHGIEKPGLIAPESH"
     gene            complement(41054..42382)
                     /locus_tag="PKL17_02425"
     CDS             complement(41054..42382)
                     /locus_tag="PKL17_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNK43610.1"
                     /translation="MISSEAIWSFAALAPAWPAAGALLVGGLALLQNRPRENLVVAIT
                     HLSLWLTLASVLTGSVMWISQDGKPVDLRVIDIYRSGDYALELRLYFDLASVVVSMVA
                     AVLILATSRFAQRYLHREPGFLRFFALMLMFAAGVMLLVLGGSYDLLLAGWEIVGWTS
                     VLLVGFFQDRSGPVRAALRVLVTYRLCDIGLLVGAVALHGWLHSTEYHTVFPHLAMAS
                     HGGPAVFIGICMLVAAMGKSAQIPVGGWLPRAMEGPTASSAVFYGGLSVHMGVYLLIR
                     SAPLLEQAPLARLWILGIGGVTAVMAALSGQVSADAKSGLAYATISQVGLMFVEVALG
                     MPRLALIHLAAHSLLRYYQFLRTPSTLQDALARRRAGGSVPQTAGGESSALKRFLYRL
                     AIERFAIESVLDRWVARPMLAAARALGRVENAVVTWLSGDGASKEDGHAN"
     gene            complement(42379..45447)
                     /locus_tag="PKL17_02430"
     CDS             complement(42379..45447)
                     /locus_tag="PKL17_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNK43611.1"
                     /translation="MTDGEASPHHSGNSRQQILRHALSHSAHYLPAQAPLEVFVHHNT
                     LHAFQSLPFFDALKVAGQRLRARMFLPEATYRQALARGRISQADLRFVLAQSKLAARQ
                     ESWPQRLPAKETLALLVMLHGLPETTPAGLGWLLGETLALSRLRDDVPEAARKRLLSV
                     QNADSVLSSPETEAAIVFALWQTCQEIAERAQLPAAKPRGPLFFREALQMLSGEDPCE
                     PVHQTLIPLAAAFLDRGEAQWTMPDRKHGFYLVWEEIMTAGLVVRPAWSSGLGRRIRA
                     HRREGYDAEHEVLSLLDELGIADDEIEEYVTQTLLHLAGWAGMFARLEQSSDPIISQR
                     AQVRLIDFLAVRLELDAMAFLDLGQRCGFSGPMNQLLPWLRERVVEEKTEEPRGPLAF
                     SLFQLGQLAGLRPQDLRSLGAGETQQLVDWLFEFSSAARQELWQKAYERHYRDELLVG
                     LVANRAARAKSGAVTPIEPPLLQVVCCIDDREESLRRHLESASAQIETFGTAGFFNLA
                     IAYQGIDDPSTFPLCPVVVTPQHKIVEEPDEQDAEGWATRQRRQARWKRVSETYKVAS
                     RSFWLGPLVTAVSGLWSAVPLLLTIFAPKVAARMRRRVSRQLLPEVRTHLSVREDGST
                     GGEGMQTGFAIPEKADRVGTLLENIGLTRRFARLVVILGHDSHSVNNPHFAAYACGAC
                     GGRSGGPNARLFARMANRQPVRELLRKRGIDIPDDTRFVGAVHDTCDDTIKVFDEDAL
                     PESHRLLLAEQMRIMQRALTENARERSRRFDAAPLGISPELALEHVDERSADLSQARP
                     ELGHATNASAIVGRRALTQGLFLDRRAFLISYDPTIDPNGTVLERTLAAVTPVGAGIN
                     LEYFFSTMDNERLGAGTKLPHNVTGLFGVMNGACSDLRTGLPSQMIEIHEPVRLHLVI
                     ENRPAVLASILERQKGVAEFVLNGWVHVISVDPDTGDMLEFVPGEGFVPWRPTSGEPP
                     AADAIPKTATWHDYYQGHRSFLPPALLSRDAAPQVGEKRRKRRFRVGGAG"
     gene            complement(45615..45995)
                     /locus_tag="PKL17_02435"
     CDS             complement(45615..45995)
                     /locus_tag="PKL17_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43612.1"
                     /translation="MRLLLARLWLLLPLLLLLQASGAAESAWPTNQQTASYLPGDSSR
                     SHGAPVEDGATVESDDDDASPRGGADAHRSAVHRGVSWSPRLWPAWTNIRLCSSGDAL
                     PLRALRLLNSPLRGPPIPRLREPT"
     gene            complement(46080..47711)
                     /locus_tag="PKL17_02440"
     CDS             complement(46080..47711)
                     /locus_tag="PKL17_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43613.1"
                     /translation="MSQSATLPTQLGRYTELKLLGQGGMGAVYKAYDPSLQRTVAIKV
                     MLGQNPEFLARFEREARAVAKLSHPNVVQVYDFGRDEQGNPYFVMELLSGRSLDSLLK
                     EKGPLPNAKVADVLRQAADGLQAAHAVGIVHRDVKPHNLMLDDGGNVKIVDFGIARDM
                     ASTDELTSTQATPGTLHYMAPEVLSGQSADARADIYALGLVGFHLLVGKPPFTGQSAV
                     AVAMKQINEPLPDLRDEAPGVPASLRRLIERMTEKRPEARVQTCGEVAREAALIAHEI
                     VSGTTVQSGYRGRSSVSRPLLVAAAALGAVAVVVMGLAMSKSGRRGPRPMAEASRQYV
                     EPPEPPTSPPPSGPPVNQATPTQGPTPPVAPGQTTAPDAPSQVGAQSPSNPLPESIVR
                     HHQGPLRVAVLRFKNLAADRELAVLVEGIGEVLMDTFANSPALRKRVKLLERNQFDEQ
                     NLVELLRSKEEYIDKSAAAKLGKILGAEVVLQGSFEKQGKLLRVVTRMTSLQDGEVLD
                     SSSEVVPYVTTSDKLRAAEIVAEKIQVRLLALAGG"
     gene            complement(47925..48656)
                     /locus_tag="PKL17_02445"
     CDS             complement(47925..48656)
                     /locus_tag="PKL17_02445"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNK43614.1"
                     /translation="MKTQIVSLLFVTLPLLASCTGADVSSGSTVSDREVAALAVAEPG
                     AKASEYQARAAEFRLFGTQGEGSEAFATLADTKTWETSNVTVGQTIGRSLKLVAVREN
                     EIDILDTVTGKPQTVRAGENVGVRIIEHEFDRAAQETASHVFHTDPRGLQRVASRYGV
                     GASCETVAFAGHSPCRITAVAKHSLADRLDLKVGDLVVSSDGQPVTPDSLASVLRSMA
                     DTASQARQLTIARHGVFLPRVYLSE"
     gene            complement(48670..49569)
                     /locus_tag="PKL17_02450"
     CDS             complement(48670..49569)
                     /locus_tag="PKL17_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNK43615.1"
                     /translation="MRLTVCLGVLLPLLVPAQLLAADPAKLAGIGDSISQGWGANNWP
                     GEHPEYGFGQGTDAKVNSLYLRYKAKNSAMTKEFVSESGAEMVGGRNNAAGQAARICA
                     MAQKPNRVVLELGGNDICSRDKGSSSDATSNLYSVTTYRNALKAALELLGGCLPAGSQ
                     VQVLSMPRVDYLYEAGNARNFLTCNLVWSTAGVCRVVTGESNAGRRAAIGARVDQYND
                     GLRAEVDAAATKYSGKVNFITDWRGNATSNSSVGTYHFGKDDIDGLDCFHPAKDTGQR
                     KLACAAWEAAEYGTLANVPSCLK"
     gene            49879..50916
                     /gene="rfbB"
                     /locus_tag="PKL17_02455"
     CDS             49879..50916
                     /gene="rfbB"
                     /locus_tag="PKL17_02455"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNK43616.1"
                     /translation="MNVLVTGGCGFIGANFVQQQRRLYPQDRLIVLDALTYCGNLRNL
                     EPLSGDRGLRFVKGDICDGDLVGKLLLEEEIDAICHFAAESHVDRSILGPLPFVQTNV
                     VGTTVLLDAARNAGIGRFVHVSTDEVYGTLSPSDPPFTENTPLAPRSPYAASKAASDH
                     LALAYAHTYGMPVIVTRCSNNYGPYQFPEKLIPLMILSAMADKPLPVYGDGQQVRDWI
                     YVADHCAGIDAALRRGQPGEVYNFGGRAECRNLQMVHSILALLHKPQSLIRFVADRPG
                     HDRRYAIDSQKAEQQLGWQARWTLADGLVETVRWYRENPAWCQSVQSGEYRTFFERNY
                     ADRLVTAKEVA"
     gene            50916..51803
                     /gene="rfbD"
                     /locus_tag="PKL17_02460"
     CDS             50916..51803
                     /gene="rfbD"
                     /locus_tag="PKL17_02460"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNK43617.1"
                     /translation="MRVAVLGPQGSLGVHLCEELRHAGHDVDPIDRSICDLRNPDREN
                     LYRLRDRLTEHDCQLVVNCAAYTDVDGAEQNTDEAFQINALGAEMVARAAGEASAALC
                     HISTDFVFDGTQSRAYDEFDEPRPLSAYARSKLAGERLVQKSGVRSFIVRVGGLYGRR
                     GRNFVSTLVARLKVNQPLRIDKQRLTAPTWNRLLSRQLITLCERGEHGVYHATCLGET
                     TWYGFAQALCEEAAALGCELPQTFIGVTTDELAAPAARPASSLLDCRMLRLRGLMRLP
                     SWRDALKGYLTELLIQGEL"
     gene            complement(51807..52775)
                     /locus_tag="PKL17_02465"
                     /pseudo
     CDS             complement(51807..52775)
                     /locus_tag="PKL17_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
BASE COUNT        11083 a        17199 c        15050 g         9685 t
ORIGIN      
        1 gcccagatcg agccgtcgcg aaagccagct ttctgcttga tagacaaagt gggtgtccgt
       61 cgatggatgc cgcagccgct gacccgccgt agccgcaaaa atgactgtcg agacgacaaa
      121 cagccacagc gcgactttgc aatcccggct cacttgccac ctcgatccgg gtcaggtcgc
      181 tgatacaccg tgcacagcgg cacgccctgg tgagtcaaca cgtagcttgg cttggtcgtc
      241 ccgtaggcat cccaaatcca atactcgtat ttgttgaagt gtttttcgtg catcaccagc
      301 gccgcccgcg agctgcgcac gcccggttct tccatccccg aatcggcgat gtctttgcgc
      361 aaaaatccgt cgagctggct catctgcaat tgaaaccagt tggcgtcgtg aaaatagacc
      421 ggggtgtgcg gcaagagcgc ctggttcaaa aacggcatga cgccgcgcac cgaataaccc
      481 caaaactgcc gattcatccc caggttcgcg gcaccgacgg ggccaccggc cagcgcgctg
      541 tagtggctca gcccgtaggg atgcgagcga tacaccgaca gcgccgccgg caaaagcaca
      601 accgcgacga ccagcggagc caacagccgc tgcacgcgca tggactcact cacctgacgg
      661 atgagatacg caaccgccgc cccggcgagc agcgccagaa acggaaccgc tggcagccag
      721 tgcttttcgg caccaaaaat cggcgtcccc ggacgcatga tgatgagcag cggccacacc
      781 gccgacagca caagcagcgg cagctcaccc gcccgctgtt caccgtctgg aatcggcgct
      841 tcaacgtccg cgcttcgcca cgtccacaca atcgttccca gcttggcgag caaaagcgtc
      901 accgccggca tcgtcaagag cgtcatcacc ggcacgtagt gccacgggta cggcgggcga
      961 ttgaagttct gccccaggta ctcgatgttg tagtggacgt ggtgcaggtg aaagctcacg
     1021 tagaacgtga agcgacgcac cgtgtcaaac cacagatacg gccacgacag aaagtagacc
     1081 aacggtccaa tcgtcgccat cgccaaagcc cacggctgaa acagatagcg cagccagcgc
     1141 tggcctttcg atccggtgcc actgcgcagc tcgtcgcgcc tcagccacag cgcatgcagg
     1201 agcaaaagcg gtggcaagaa aaacgcattg tgcttggtcg atagcgacag accaaacagc
     1261 accgcagaaa tcaccgccca gcgcagacgc cgcgtctgct gcgcccgcag gtacgcccac
     1321 gtcgaaagcc ccagcatcgc cgtcaccggc gcatcgaagc aggacagctc ggcatggaaa
     1381 aaatgccgcg gtgccaaaat ggtcagcccc gctgcggcca gtgccccggc tcgcccaaac
     1441 agccgccgtc ccagaagaaa caccactgca accagcgatc ccgccagcgc atgcgccggc
     1501 agacgcatcg cttcgccgtt tttcagaagc cccagcgtcc gatgtcgttt cacatacggc
     1561 agcgcgtgca tcccggtttc cgacagacaa ttgcagcgat gcagcaaacg ccacgacagc
     1621 ccaaacagcg tctttaagag cggcggatgc tcgttgttgt acgaaaaaaa gcggtcaatc
     1681 aggcctcggt caaacagcgc tttgccgtcg ccatgcgcaa gccgggtcgc cagttcttcg
     1741 tagtaagcag catgctgctc tgcggcgtga aagtaatagc cttcgtcgcg agcaaagcct
     1801 tcgcgccggc tcgcaagctg gagcagccct gtcgtcgcaa caagcaggag cagcgccagc
     1861 gtcagatcaa gccgggactt gggacgattg ggtggcgcgg cgctcatcgt cgcagctctc
     1921 cgtgaagacc cagcaagtga tgcggtgcac tcgccgcaga caactcgatg cgcaggtgat
     1981 gcacgtcacc gctcttcgca ggcagcggca gcttgtgcag cacaaagccc tgcccgacct
     2041 gaacttgcag cgaatgtcgc gccgatccat cgtcgacatc gaccaccaca tcgacaggcc
     2101 ccaccgcttg cttgcgctgg tgatagtcgt gcagacccag ccacagatgg agcgtgctgc
     2161 cactatagtc tgcgtcggga atcgaaaaac gcagcagcgt cttttgacct tgcgcaagct
     2221 cgctcacgat gccccgccgt ggctgatagt cgatctccat cgtgcgacgc tcgacccgtc
     2281 cgacgctgca gacaaaacca cccagcgacc caggggctgg cagaggcgtc ggcaaagccc
     2341 ccgtccacaa acacggcgat tccggcgctg ttcccgcaag cggaatttga ctgaccgtcg
     2401 cgtccagaaa atgcgcagat aggtcgtaac ttaccgtcgc tggcgtctgt gtgtagtggc
     2461 gcacgcgaag cagaccaaag cgcagttctc catcgctgtg tagaccgtca acatcagcgg
     2521 cgtgatgggg cgaaagaaat atctcggtga tcccggcaaa gcgcttgccg tcggcacggc
     2581 caaggtgttc gaccggcatc aggtcgccca gctctcgctg gatgagagga gacagccacg
     2641 ccggcgaaac cgctatcagc tcccccggct tgtgcgcagc ccgcaccgct tgcgccgcac
     2701 tccgaacatc caaatcggat atccgcggcg gaaaaagcga agcccagctt tcccacaggg
     2761 cgacaagtgc gacgccagcg agcccaagcc gaggaaacag acgcgacaca gccagctacc
     2821 acacctggca ctggttcttg cgaaccatct tcgccgtcgg agcgcatttc cacgcagacg
     2881 caaagtcagg caggttcgac agcggaccgt tcacgcgaaa ctccgccggc gagtgcggat
     2941 cgacgttgat aagcaggcgc gaatactcag gccgccgctt ttcgcaccac gcttgcgcca
     3001 ttcccaggaa gaacagctgt tcctcgctgt agctgccttt gctggccacg ggattggtct
     3061 tctgcgcggc acgatacgca agcagtgcca gcttaagacc gcccaaatcg gcgatgtttt
     3121 cgcccagcgt cagcttgcca tcaagattgg cgtcgggaag cggcttgtat tcgttgtact
     3181 gatcgacgac gcactgggcg cgccgattga actcgctgtt caccgactgc gaccaccagt
     3241 cacgcaagtt gccatcggca tcgaactggc ggccttcgtc gtcgaagccg tgcgtgactt
     3301 cgtggcccat gaccatgcca atcgcgccgt agtttatcgc gcgggcgacg ttgcgaccga
     3361 acatcggcgg ctgaagaatg cccgccggga acgccatttc attgaacagc ggctcgtagt
     3421 aagcatttac cgtcggtggg gtcatcagcc attcgttttt gtcacgcggc ttgccgattt
     3481 ttgcaagctc gcgacgggct tcaaacgccg acgctgcgct caagttggca agatgcgatc
     3541 ccggctgcgt cttcagcgca tcatagcttc gcggcttgtc ggggaaaccg atcttgttcg
     3601 cgatccgcgt cagcttttcg agcgccgcct tgcgcgtcgg ttcatccatc cacggcagcg
     3661 ccttgacgct ctgttccatc gccccttcga tgccgtggat gatctccagc gctttggctt
     3721 tgccatcgtc gccaaacgtc ttggacacaa acgccgtgcc cagcgcttcc cccatcgcgt
     3781 ggtcgacgaa accgacacag cgcttccagc gcggcaggat cttgtcggtg ccggtcagcc
     3841 gcttgctata aaacgcgaac gtctcgtcga caaacggctt cgacagtgcc atcgccgacg
     3901 aatgaatctg ctgccagcgc agatacgtct tgatgttttc gagcttttcc tcgacgacca
     3961 gcttgtttag ctctttgaaa taatcgagcg tcgcgacgtt tatctctttg accgtcggaa
     4021 tcccgagcgc ggcaaagtag gcgttccact ggaagttcgg cgccagcttc agcacaccgt
     4081 cgatgtcaat ccggtgatac agattcttcg gttcacgccg ctcgacgcgc gacatcgaag
     4141 cttgggcgag ccgcttctca atcgcaaaga tcgtctgcgc ctgcgccgcc gcgctttcct
     4201 tgctcacccc cgacagcacc agcatccgct cgacgtgggc aaggtactct ttgcgcagcg
     4261 cttcgctttt ttcgtcggtc ttcaagtagt aatcgcgatc cggcagcccc agtccacctt
     4321 ggtcgaggtt gccgatgacc tgattggcat ttttgaagtc tggcgacgcc gaaaaatcga
     4381 acatcggctt gccaatcccc acatgttgcc gcgccagctc tttcgccaga tcggcctgcg
     4441 tcttcacgtc gccaatcggc ttgagcagcg cttgcaggtc cgccgccgcc gtcttttcga
     4501 tggctggctc gtccatacag gccttgtaca gatcgcttag ctttttgctg ttgggatcgc
     4561 tacccggttt ggcaatgttt tcgtcgagca gctcgcgcag cacggtctgg ttgcgttcgt
     4621 tgatttcgga aaaaccgcgg ctccatgccg gacggtccgc cggaatgtcg gtctttttga
     4681 tccagccacc acaggcgaag cgatagaagt cgtcgcacgg gttgaccgcg cggtccacga
     4741 tgctctcgtc gatgcccgag gcatttccga ccggcgattt gggctgcgcc gaagtcgtcg
     4801 aagctggcaa aaagacgcta cagagcagca aggaagaaaa agcggagagg gtggcgcgag
     4861 tctttgacat ggcgggtcat ataccaccgc ttctgggatt tcgccgcttt gatttgccaa
     4921 agcttagcta tctggacgca gatgtgatgt tggctcggcc ttgtgctcga cagcaacggc
     4981 tggggcttgg gctgcacgcg cttcggcaaa gacctgacgc accgacgcaa gtggccccag
     5041 aagcagaaac ggtccgacga ccgccaagtg atacaagagc gcgcccgcca gggccgtttc
     5101 ggcggggact ccgagcggac gtagcaccag catgaccgtt gcttcgacga caccgaaacc
     5161 ggctggcgtc agtggcaaaa gcaccgccag gttcagcgcc acaaacaaga gcagaagcac
     5221 atgcaacgac accgaaacac ccgccgcctg caagcacaag tgcagcatca ccatgtcaaa
     5281 accgtcgagc accgccgtcc acaaaaggct cggtccggaa attttcggca tgggcggcag
     5341 acgacgccgc aaaaatggca ggtcggacaa aaagaagccc aggctcgcag cgaccccaag
     5401 cacgcaggcc acgatcaccg ccagacggat cgccgccggc aattccccgg tcagcagcgc
     5461 caaaaaaccc cacagcccca ggctcatgcc atcgagcagc ttttcgacga gctgttcacc
     5521 aaccagctcg acaccgccga aaccgtagcg ccgtcgcagc accaacagcc gaaaacccat
     5581 ccccgccggg ggccacagca ccgtcgcggc ctgcgcgctc accaagtcga cgtacagacc
     5641 cagaaaatgc ggtgcttgcc cggccagcga ccgcagcaaa acgcgcgtcc gctcggcccg
     5701 cgcccccgct cgaatcgcga cattgagcag cgccgccagc acccacagcg agatgtgact
     5761 gcgcccgatc gactgggcaa aggctcgcag atccagtcgg agcagtgccc agaacaaaag
     5821 gagaaggagc agaatcaggg ggccaatccg tcgcaaaatc cgcatgatcg cggctccact
     5881 gggtcgtttg tagcgcgcag tcaaagcagc cggacgctgc gtgctggtta tcttgtgtct
     5941 tggatgcagc cccgtggctc aaagtacacg gcaatcgcac tgggtcggtt gctgccatcg
     6001 agttcacaag ccaaggcgat accagcgctc accaaagcat caccgcgtag ctcgatagga
     6061 ccgctcagcc gctccacttt ctgtaagcgt agcccgtccg gaaccgagat tcgaatttct
     6121 ccgtcccctc ttgtgcatcc cgcgatcgtc acagtgagcc gatttccgtc ggcatcccac
     6181 gtttcagact gcagaagctg aagcgtctgc cacacatgat gcgtgaccga cacgacctgc
     6241 gggtgcggct ttcgtcgtcg caaaagccac agcgccgtgc catgcggagc cagccgcagc
     6301 gtcaaggttt gcaaaggatc aagctgcgcc atcgtccgcg accacagctc aaacgccacg
     6361 tagtcagctt cgggcagacc gatcgcttgg gcagaaagca caacctcgcg cccgctgtcg
     6421 tgccagttga taagcgccgc cagtgccgcc cgatcaccgc tgggttcgtc gccgacaaac
     6481 acgaagcgct gcggtttttc ttcgcgcaac accccgagcg ctcgcgccgt ccgaccgctc
     6541 ggtggcagtg tccgcgaaag tagctgcacc cgttcgccgg gcagcacccg cagatcatcg
     6601 ctacacagca gcatgccgcc agtcagcgac atcaccgaca agagcgtctg cacttcgggc
     6661 aggctcaagc tcgacttgtc gcgccgcaca agcaaacagt cgggatcgtt acaccaccat
     6721 tttccgtgca gatgggcccg cgtaagacag ttgtgcagcg ccatttccgc cgacggatag
     6781 cccgtcccat gcgcgatcaa atcgaccaaa tcgtcgcgcc agtcttcttt gacatcgggc
     6841 gaaatccgca tcgcatcgac aaaaccaatc gacggcacaa gcggcgcccc acaaccgagc
     6901 aaaaaacgat cgcctatcgt ctcgcgaatc acccgcaggc cctgccgata cgcttcgacc
     6961 gcagacagct gtggattgtg ccgaagtccg cgtcgcagcc cggcgaacaa aaagtcgatc
     7021 ttgaaatact caaagcccag ctcgcacagc gtcgaaaaca cccgcctaag ccacgccagc
     7081 accgccggat gggtcaaatc gagactgtag acctgtccgt cgggaattcc ccaaaacgga
     7141 tggtaaccaa gccgcacgag cttgccgtct tcgtcgcgca gcagccagtc gcggtggtgc
     7201 tgaaaaattt ccgacgagcg ctgcacgaaa aacggcgcca gccagattcc cggcacaaaa
     7261 ccggcttcct tgatctgccg cgcaagcccg ccgagacccg acggaaactt ggcattggtg
     7321 gtcagccagt caccgatttt gcgctgatag ccgtcgtcga gctgaacgta ctttaccggt
     7381 agtttttttt ccgcaagcgc cgtcaggttg cgcaggacat ctcgctcgct gactttggtg
     7441 tagtagtaat accaagagca ccagccgctg gggaccggac ttggcgacgg acgcggctgc
     7501 atctgacgtg cggtttcgtc cacatactcg tcgagaatgc cttgttcgct gtgcgacagc
     7561 gcaatcagca gtttgtcgcc cgtcagggtt tctccgaccg caacctgccg accttcaaaa
     7621 ccaagccgcg cctcgatccg cggggctccg tcgcgacaaa tcgccacttc gccggttccg
     7681 cacagcgccg aaacaaagcc aaccagcaag tggccaagcg gctgcgcagg cggagtcagc
     7741 accatcatcc acggcgacga cagacaaccg ggtttcccgt agtagtccga ttcggtcagc
     7801 agattgaacg aagctgccgt cggaaacagc ggtttttctt cgcgaaagcg actgtcgacg
     7861 gaaaacgacg gcgtaaacga gctatagcct gtgcgaaaga cctgaaaacc gggctgctca
     7921 ccgagccccg caacgaagct tgtctgaagg ccgagcgcgg tcaaacgaaa ggcagacaac
     7981 cgtatcggct caccgctctg gttgcgcagc gactgccaca aaagcagccc acggtggtcg
     8041 agccgggcgt gcacaatcca cagaagtccc gacccgtccg ccgcgggaaa acgcagctcg
     8101 atggagccat cgggaccgtc gaccagcgta taaagcgccg cctcggatgt gaccaaaagc
     8161 gcccgtccgc ccggcccttc cgccgtgact tggaacgcac ccaaaagcga ctgtttttcg
     8221 gcagtctccg tcgcagaatc ggcctgatcc gatagcagcg aacgggacgc aaacagctca
     8281 aacccggcgg gcgaaagcca cagctcatac cccgcgtcac acagcagcct gcgcacgact
     8341 attccccccg tcccagcttg cgcaaagaca gcgcccgcag tcggtcttgc agcgcgcgct
     8401 catctttggg aaacagaaaa accagacaca aaagcaccac gccacagccg agccacatca
     8461 agttggcaat gtgaaatgcg ctcacgcgac ccagccgcgc cgccatccca ccgacgatcc
     8521 aagcccccag accgcggccc aagtcgttac acaggttaaa gaaactaaag gccgaccccc
     8581 gtcgctccgg tgggttgacc gcgatcagca tggtgttgac gttcggtccc gtcaccgccg
     8641 ccaaagatcc cgtcagaagt ccgacgatga gcggccccag caggctttgg ccaggacgca
     8701 ccgggtattc gataagcacc gccatcggaa tcaccgccaa aatcgtcgtc acggcacaca
     8761 gcagcggcag gttgcgggcc ttgcgattca gaagcttttg cccggcaagc ccaccgatga
     8821 agccaccgac gatcgccgca atcccgaccg acatcacgag cagcgtcgct tccggcaccg
     8881 aaaagccctt gtcgtgcgcg tagtagtcgt ttaggtacac aaaaaatacg ccccacggca
     8941 ccgtccccgg cagcgcttgc aacagaatca gcaggttggt ctttaccgca aaaagctgtg
     9001 gcaagtcgcg caggcggagc cgctcttcat aggcttttcc cgacgccagc agatcgtgca
     9061 gaccctcttc gtgctgaccg cgccgcggct ctttggcaaa cagcgcaaac agcacgctca
     9121 gcgcaagtcc aggcagcgcc acgacgtaaa acggcatccg ccagcccgac tgcgcaccga
     9181 gcatccccga caaaagctgg cccagcccaa tccccagtcc ctgcgaaagc ccgagcgtcg
     9241 ccgtcgcgct cgcccgcttg tgcggcggat agtagtcccc gatgagcgaa tagatgagtg
     9301 gaaaggcccc gccgatgccg attcccgtca gcgcgcgcgc ccacaaaagc tggtcatacg
     9361 tcttggccag ccccgatcca agacacgcaa gctgaccgat gacagcgacg atggtcagca
     9421 accatttgcg cgacagcttg tcggccagat caccgcgatc agccaagtat ccgacgatca
     9481 gcgtcagcac accgccgagc atccaaaaca gcaagttgac gtccgccccc agtcgctgat
     9541 cgatttcggc acgagaaaaa ccaaactcgt tgccaatcgc ggtcaggttc ggcgccaaaa
     9601 ggttctgatc tgcgaaaagc agtccgacga tcaacacaaa cagaatctgg gtcacgacca
     9661 ttcgagctac gcttcctgta gccaaacgtg tgcttggaag cccctcgcca cttgcgctgc
     9721 gaccgccgct gtcgttcatt ctttgcaagc taccacagcg gcccgccaag caatctgtca
     9781 ggctgcttcc gtcgcgctgg gcagaccccg cgctccccac gtacactgag cagatcggta
     9841 cacttgtctg aacgatccgg tacacccacc aggaatccgt accgacccac aaaggagcgc
     9901 gaaagtgaaa ccacccgact tgtgccgtcg actcctgcct tggattgtga gcggcctgtg
     9961 tggctgcctg cattttcagc cgatcgatcg cccgctcggc gacgatctga acgaacgcgc
    10021 cgcgctgatt gatcgcgccc aacatggcgc aatgggccaa aggcagcagg tgctggttgc
    10081 caaagcactg atggcgacgc acggcgagga actgtcccga ctgaaaagcc ttatcgatcg
    10141 cggtacagac ggggcgaccc tgcatcggct gatttaccac gacgtgtccg acccagtgct
    10201 aaggcaagcg ctggtcgccc atctggcccg tgaagcccgc agcaccgacg cgctcaccgc
    10261 ccatgtcccc cgagacaaag tgcgggtgtt gtccgatatc gacgatacgc tggtgcgatc
    10321 gctctgcgac gagcggtatg aaaaacacac tcttttgccc ggtgttttgg agttttaccg
    10381 cgcgttatcc gtcggtgcgc gtgcccgcga agtcgaagcc caagcgcccg gcgaagtgac
    10441 ctttctatcg gcgcgaccca aagagctaag cggtctcagt tattcgtcgc tgctaaatcc
    10501 actgctttcg gcgcgagggc agatcatcgc gggctacaac ctgctgcccg ctcccgctgc
    10561 tgtacctgga attggcaatg tccacgaagt gcaggcattc gccaagttcc gcaatttcgt
    10621 cgagctggcg acgctgtatc ccgaatccga cttcgtcttc attggcgaca gcggccaagg
    10681 cgacatgctc ggtggtgagc tgatgctgta tcaccagccc gaacggatgc gtgcggtgtt
    10741 catccatcag atcatcaccg acggaaaaca tggcatcctg tgtccctatc cacgcagcca
    10801 aagcgatctc gatcgcggca caagtcgcgt ggtctttttt gacacgtaca tcggcgccgc
    10861 cgcacaggcg cacgaatggg gactcatcag cgaagactcg ctttatgtcg tcaccaaaag
    10921 cgccatcgag acactgaacc agcgcggcag cgaaatcctc actgccaagc tgcgcaacct
    10981 ggtgattgcc cagttccgac gggacattcg ccgtgtgcag gaaatcctcg ccaaccatcc
    11041 cgtcgacaca gcgcgtgacc ggctgcctga tttgctgctc gcggaatcaa gctgcgaaaa
    11101 aaatccagcg acgaagaatc cgtaaaatcg aatcaaactg cgccaaggcg gcatcaagcg
    11161 gagaccaccg tccgcgcgtc ggttcaattc gatctgtgct aagggttttc gccgtgaatg
    11221 aagcagctct ccaagacctt ctctcgcaag tggcgacggg acagcttgaa gtccaagccg
    11281 ccatgcagcg tctggccaag cttccgtttg ccgatctcgg gttcgcccgc gtcgaccacc
    11341 accgcagtct gcgcgccggc ttccccgaag tcgtgttctg ccaaggcaag cgccccgaac
    11401 acgtcgtcgc gattttccgc gagcttttgt cccatggcag cgccgcactt ggaacccgcg
    11461 ccagcatcga acacgccgag ctttgccagc gcgagctgcc ccaagtcgag taccacccaa
    11521 gtgcgcgact cttgacctcg ccccgtccca gcctgccggg accgccgctt ggacccgtgc
    11581 tggtcgtctg tgccgggaca tccgatctac ccgtcgctga cgaagctgcc atcaccgccg
    11641 aatttctggg ctgtcacgtc gagcggctct gtgacgtggg cgtggccggc atccaccgac
    11701 tgctcgcagc cgaagacaag ctccgcgccg cccgcctgat catcgcagtg gccggcatgg
    11761 aaggcgcgct tccgtcggca gtttccggac tatcgggaaa accggtcatt gccgtaccga
    11821 cttcgattgg ctatggcgcc agcttttccg gtctggcagc gctgctcggc atgctgtcgt
    11881 cgtgcgcatc cggtgtcacc gtcgtaaaca ttgacaatgg ttttggggct gggtacgcag
    11941 ccgccctgac ccttcgctca gcgacgatac ctttgaccga aaccccacgg agttaggccc
    12001 catgaccgca acttgtccgt ctccaaccag cccggatttt ggtgatccat cgtcgctgcg
    12061 cggctgcacg ctttatttcg actgcgcgac ggggctggct ggtgacatga cgctcgccgc
    12121 gctgctcgac ttgggtgtgc ccgagtcatt tgtccgcgag cagctgtcgc ttttgccgct
    12181 ttcgcactgg gaactgcaag tcagcagcgt ccgcaaacat ggcatcgtcg ggaaaaaggt
    12241 cgatgtgatc gatctcggag cgcccctgac cgaactttcg cacgagccga agcaccacgg
    12301 gcacgatcac gcgcattctc atccccacga tcacccgcat tccgatcacg accacgacga
    12361 cccccatcac gacgcgcacg gcgacgatca cgacgacgat caccatcatc acgatcaccc
    12421 gcacacccac tatgccgaca ttcgcaagat gatctcgtcg tcgggggtgc ttgccgatgt
    12481 gaaacagcgg gcgctggcga tgtttgacaa gctggccgcc gtcgaagcag cgctgcacgg
    12541 cgtgacgatc cccgaggtga tgtttcacga agtcggtgcg ctcgattcga ttgtcgatat
    12601 cgtcggggtt gccgccgcat tgagctggat ttcgcccaaa cgagtcgttt cgcggaccgt
    12661 gccgctcggc agcggacggg tcaagaccgc ccatgggctg ttaccgattc cggccccagc
    12721 gacggcagca ctgcttcgtg gtgcccaggt cgaagccggt gatctgctcg gtgaactgac
    12781 cacaccgacg ggggcgctca tcgtttccag ctatgccgcg ggttttggac cgctgccgcc
    12841 gatgcaagtt ttggcgacgg gccacggggc cggcacccgc gagctgcccg atcgtcccaa
    12901 catcctgcgg atcatcgccg gaatggaacc gcaggcgaca accgcaccca cgcataccga
    12961 cgacacggcg ctgtgggaaa tctgcgcaaa cctcgacgat ggcaatcctc agcttttgcc
    13021 accgcttgtt gattcgctgc tcagcgtcgg ggccaacgat gcctggctgg atcaagtgat
    13081 catgaaaaaa ggccgaccgg ggaccgtgct gcatgtactt tgcgacgaac agcagcgagc
    13141 cgagatcgtc cagctcatcc tgcgtgagac gacgacgatt gggctgcgct tccaccgggt
    13201 cgagcgaacg atcttgcagc gcagcatcgt ttccgtcgac acagcattcg gtcctgtgga
    13261 tgtgaagatc gggcgcgatc cgcacagcca acagattttg aacgtcgccc ccgagtttga
    13321 atcggtgcga aagctcgccg ctcgctgcaa cttgccgatc aagcaggtct acagcgcggc
    13381 gattgccgca gcggaaaaac agcgaccgac tacaaattga tgatcacgac cccacgatcc
    13441 ccacttggga tgtcatgatc atcgcgatcc tcgtcgctgc tatcacgcgg cggcggataa
    13501 tccggaatcg gcacgagcgg ctgacgttca tgtcgccgct gctgctcttg ttcccgtttc
    13561 cggatttgtt cgatgatcca gggctccagc atcgcccact tctttcgtct ctcgtggctg
    13621 ttacttccag tttgagtctc agagagcgaa gtcgtcaagg cttgcgccca atcttccgca
    13681 gaagaatcga cgcgacgggc catttgacat gcccaaggta gccaagtacg ctccctgccc
    13741 ttgccaccac catgtccggc gctcccgttg agatcatagc tttacgaaag accttcgcat
    13801 tcggcggacg gcccatcgaa gtgctcaaag gcattgatct cacgatcgac gccggcgaaa
    13861 tggttgctgt cgtcggcgcg tctggcgcag gaaaatcgac gttcctgcat gtgctcggaa
    13921 cgctcgatgt agcgtcatct ggcaaagtca tcattgacgg ggccgatgtc acttcgatgc
    13981 cgccgacggt gctggcgaac ttccgaaatc acaccatcgg ctttgtcttt cagtttcatc
    14041 acctgctacc cgagttttct gccgtcgaaa atgccgccat gccggggctt attgccggac
    14101 tcgatcgccg ggtcgcgcag tctcgcgccg aggaacttct gaagcgtgtc ggcctgggtc
    14161 atcgacttgc ccatcgtccc ggtgaactgt ccggcggtga gcagcagcgt gtggcgctgg
    14221 cccgagcctt ggtgatgcag ccccgtcttt tgcttgccga tgaaccgacg ggaaaccttg
    14281 atgcgcgtac cggcgcggcg atccacgagc tggtcgtcga gctaaaccgc gagcgtggca
    14341 tgacgatgat tgtcgtcaca cacaacgaag acctggcagc ccgaatgcct cgccgtctac
    14401 acatggccga tggtcgtatt ctcaccgacg aagaagcgaa gcggcacgca accgccgtgc
    14461 aggaaggtgt cgcttgaagc ggtcgttttg gattctcctc gctgtttggc tcggcttcac
    14521 attgccggcc cagtcggcgt ctgccgacgt tgagctgccg attggccgtc gcgtcgaaaa
    14581 aattggattc aagggcaaca tcaaagtcga agccgcggcg atgcagcggg cgctgtcgac
    14641 ggaagaacgc tttggcaagc accccgtcga agaagggcag ccgctcaagc tcgatacgct
    14701 tcaggacgac ctaaaaacgc tgtggaagat gcggtcgttc gacgacgtgc gcatcgagtg
    14761 ggaagaaggc gactccggcg gcatcgtcct acagtacgtc gtccgcgaaa aacgcacggt
    14821 tcgcaaaatc tacgtctcgg gcaacagcga agttggcctc gacaagatca acgaagtcct
    14881 cgatctcaaa aaggacacga tttacgaccc ggcgaagatc aagcgcaacg tcgaaaaaat
    14941 ccacgatctc tacgtcgaaa aaggcttcta cctcgccgat gtcaaatcgg acatcaagat
    15001 ccactcggat cgccagcttg atgtctactt catcgtcgac gagcacgcca aagtcgaagt
    15061 ccgcaaagtc accttccaag gcaaccgcaa tgccacagac gaagagctgc gttcggtcat
    15121 ggcgacgcag gaaggcaact ggctgtcgtt tttgacgtcg gcgggcacgt acaaagaaga
    15181 cgctttcgag cgagacgtgc tgctgctcac cgcgttctat tacgacaagg gctacgtgac
    15241 ggtgaaaatc ggcaagccca ccgtcgaaat gtccccggac aagcgcttcc tctacatctc
    15301 gattccggtc gaagagggcg agatttacaa gctcggcaag ctcgacttcc aaggcgagct
    15361 gctcgtcccc aaagaagaca tgatcgcggg tctcaaggtc aaatcgggcg accagttcaa
    15421 tcgctcgaag cttggccaag acatccagaa catcaacaac ttctacaaaa accgcggcta
    15481 cgccaacgtc aacgtcacgc cactcaccca gattgacgcg gacaagcgca tcgtcgacct
    15541 gacgttcgac cttcagcagg gcaaaaaggt cttcttcgag cgcatcaaca tccgcggcaa
    15601 caccaagacg cgcgacaaag tcatccgtcg cgagctgaag atcgtcgaag gcgaactgta
    15661 ttccgaagcg ctcgtcgagt tttccaaaaa ccgtgtccaa gcgctcggct tcttcgaaaa
    15721 agtcgaaatt tcgaccaagc gtggcaccac cgacgaacgc atcgaggtca acatcgaagt
    15781 aaccgaacgt ccgacgggaa ctttccaagt cggcgccggt ttttcctcga ttgagaactt
    15841 catcgcccaa gcccagatcg cccaaaacaa cctgtttggg cgtggcacca cgctgcggct
    15901 ttcggcgcaa gtgtcgtcgc tgcgtcagct gttttcgata aactacatcg atccctactt
    15961 cctcgacagc aacgtcacgt tttcgttctc tggctataac cagctgctct actacccgaa
    16021 cttcaaccgg acgacgcgcg gtggcgatct gacctggggc tatctgtttg gcgattacct
    16081 gcggctgctc gggacgtaca agctggaatg ggtgtcgatc ggccaaaaca cccaaggcat
    16141 gcagatttcg ggcttcgggc aggtgcagtc gatcgccggc ggaacactcg ccaacctgta
    16201 tcgtcgcggc tggacgtcgt cggtgcgact gtggacgcag tttgattccc gcaacgaccg
    16261 gatgttcccg acgaagggca tgttccatct gctttcgctt gaagtcgctg atcgctatct
    16321 ggcgtcggaa atcgactacg ttcgcgtatc cggctacgcc cgcttttatc gaccgatctg
    16381 gggaccgttt atcttccgtc tgaacgccga aagcggactg gtcttttcgc gtcgcgccga
    16441 aggtgtgccg atttacgagc gctacttcct cggcggcatt cagtcgattc gtggcttccg
    16501 actgttttct cttggaccac ggattcagat tcaaagcgcg ctcgaaccgg gttcgtacct
    16561 gattccgttt agcgtcggtg ccaactggca aatcatcatg aacagcgaaa tcgagttcat
    16621 gatcatcccg gcggtccaga tcaaaggcgt cgtcttcttt gatatgggca acggctacaa
    16681 cctcgagtcg aaatactgca cgagcgcggc ggcgacatcg gcactgcccg aggtgttcaa
    16741 cccgtgctcg cactatccga cgttccagaa cctgcgctac tcggttggct ttggcttccg
    16801 ctggttctcg ccgattggtc cgctccgctt tgagtggggt attccgctca acaaacagac
    16861 cggcgaagac ccgctggtct tcgagttcac catcggtaac ttcttctaaa caatgcgcgt
    16921 ggctgacccg tcgaagaagt cgccacgcag cccaaaatga taacccccaa aggagtctga
    16981 caagatgttt aatcctaccg tgcgatggac tttaggcctg ggacttgccc tgtcggtcgt
    17041 gctgcccgca gcgacggcaa aagcccagca gaagattgcg atcgtcgata tccgtcgcgc
    17101 catcgaggac agcaacgaag gcaaggcgaa catcggcgga ctgaaggccg aagccgaccg
    17161 caagcagaag gaacttctgc aaaagcagga tgagctcaag aagctcgaag aggacttcct
    17221 caagcagcag tcggtgcttc agccagcagc ggcgcagaag aagcgggaag agcttcagca
    17281 gaaggtgatg cagcttcagg atgcgctcca gcgggcccag caggacatcg ggcaaaaaga
    17341 agccaaggtg cttcagccgc tgcaagacaa ggtgctgcgc gcgattgatg cgctggcgcg
    17401 gcgcgacaac tacaacatgg tgctgcgcag cgatgtggtg ctgtggccgc agcagagccc
    17461 gatggacatc accaacgagg tgatccgcaa agtcaacgac atgaaggcgc cgacgccggc
    17521 accagctggt ggcgcaccga cgggcgggag caagtagtcg tggccgactc gcctgctctg
    17581 ccgcttgatg tgacggcgat catgcggctc ctgccgcacc gctatccgtt tttgctcgtc
    17641 gaccgggtga ccgagtttgc cggcgagacg tgtcgcgcga tcaaaaacgt caccatcaac
    17701 gagccattct tcgtcgggca cttccccggt catccggtga tgccaggggt gctgatcctc
    17761 gaagcaatgg cgcaaacggc ggcggttcat gcctgtctca agcttggcaa gcaggccggc
    17821 gagcaagcgt tttacctggt cagcatggac aaggcgcgct tccgcaagcc ggtggtgccg
    17881 ggcgatcagc tcatcctgac gacgacgctt ttgcgcggac gtggtcggat ttggaagttc
    17941 cgcggcgaag tccatgttgg tggcgtcctg gtttcggaag ccgagttcat ggccacgctg
    18001 ccgtcggccc tcgcgcagga tgaagccagc ggcgattccg aaagctagcc aggccagcgt
    18061 cccgtcgcct cttctgctct gtcaagattg gaacatgtcg cgccacaccc tcctctttct
    18121 cgcggcaatg ggactgtgcg cctgcaatcc acgcaagccg ctgccggtgt cgaaagacat
    18181 ggggccggcg gtgcaagtgg tcgatgccgc accgcgtcca tatgcaggaa cgcctgccac
    18241 caaggcggcc ccaccgcttg ccgagacaga accgaacgac gatcgcgaac atgcccagcg
    18301 gattgacccg cagaaagtga ttcaaggatc gctgctgccg ccgacgacaa gtggagccgg
    18361 caagggcgat gatgactact ttttgtggcc cgcccaacct gcggcccaaa cgctgcgaat
    18421 tgaagccagc ggcgccccag aactcagcct cgaggtgttg acggaaaccg ccacgctcgc
    18481 agtcatcgac gaacgaggcg ctggcgaagg agaacggctc gctgggctgt cgatccgcgc
    18541 cggacagtct gtctggattc gggtccgcgg acgggccaaa gcagaagctg cgaccacgag
    18601 cgcgctgggc gaatatcagc tcgccgtcac ggcaacgccc gcatcacccg acagcgaagc
    18661 cgaacccaac gacacagcga ccgacgcgac gcccatcttg ggaaatgatg caagtggcgc
    18721 attgtcattc cgtcgtgacg aagactactt tgtgctgccg ctgccgacgc tgcccggtcg
    18781 aaaagtcacc gctttcccga acgcggaagg cctgcgtgag cccgcgattc tgcgtgttga
    18841 gctcacgtcc ccgtcgatac tgccggcgct gcgggtgctt ttggaacccg cgctagagcc
    18901 tggtcgcgat ggtggagtgg ctgcgcccag gcttttggtc gacgtaagcg caggcaaagg
    18961 caaagaagag ctgcggctgc gcaacctcac gctgccagcg ggaaccggtc gcgtgttcat
    19021 cggggtgcgt gggcttgcgt ttgccaaacc acccggtgaa agtcgctacc acctgcgggc
    19081 gctcgtcgag acaccgctcg agggcgccga aaccgaaccg aatgacagct gcgcagcgag
    19141 tgcaagtccg ctcacgctgg cgtcgggaac ggctgacatt gcgggctttc tgtggcccgg
    19201 tgacgtcgac tgctaccgcg tgacgggtgc ggctgcgcag gaaacgacct atgaagccaa
    19261 gctcagcctg cccggtggtg actgcagcgc cgtgctggaa tgggtcagcg ccgacggcaa
    19321 gccagttggc gccaagagcg ttggcaaacc cagccagtcc gccaaagccg ccgatggcaa
    19381 gaacaccgac ggcaaaaaca ccgaactaac catcacgacc ggcggcgact ttttcttgcg
    19441 agtgctcagc cgtgaccgtc gctcttgttt cgaagcgccg tatcgacttg gcgtcaccag
    19501 caaagaaccc ggtggcgggg acaagccgtg acccgtcgca tcgtgctgtg cctcttgctg
    19561 actgcgtgcc ggaccggcag cgatcccaag cagtcgctgg agagctatct gcacgacacc
    19621 agccgcggca gctacgaccg ggcctatgac cgcttgagct tggattttcg ccgtcgctgt
    19681 gacaagccat gtttcgttcg catgcttgaa gcacagcccg gcgcaagtcg acagctcctc
    19741 gatgagctgc gcagcggcac gcaaagcgtc gtctaccagg ctgaactcaa gctccgcgat
    19801 ggcacccagt tggcgctgga acagcagcca ccggacaccg aatcggcgcg cagagcgaca
    19861 ccgccgccgt ttctgtttgc cgaaagcccg ctcgatttct acccgcaaga cagcccgaaa
    19921 aatgccctgc gctcgtttgt gcgggcgttt gcggcgcgcc gtttcgacgt gcttatgcgg
    19981 tttgttccca agtcgctcga aagcaagctc acggctgacg cgctgcggac acgctttgac
    20041 agcgagccgc ggatcgcaag ccaagtcgac gcactgcgcc aacatcttga cgacaaggtg
    20101 acggtggatg gcaacacggc acggcttgtg accggacccg agcaagaagc gacgctgatg
    20161 tttgaagacg gacgctggcg ggtgcaaaag ctggaatagc gcccggtttg cgtcagaaac
    20221 ctacgccgac cccgaactta tcgaaaagat gccatgacat tgacctcccc gacggttccc
    20281 cttgtctttc gctatggtct gcaccgggtg cgcgatgcgg tgcgggtgct accgcaggcg
    20341 gcgtggtcgg tcgaaagtca gcccgatctg tgcggcggac gggactatga actgctgatc
    20401 gacgtcgaac gactgaacat cgacgccgca agctttcgcc aaatggaaga agcggcgcgc
    20461 tcggtggggg aagacatcga agccggggtg cggcggcaga ttctcgatac ggtgcacaag
    20521 cgcggcaaga tgcacaatcc gatgaccgga tcaggaggca tgctgctcgg gcgggtgcgc
    20581 cacgttggtg gaaaactgcc cgatccgcat gcgtcgctgc ggcccggtga gcgcgttgca
    20641 acgctggtgt cgctgtcgct gacgccgctc cagattgaac gcatcctgag tgtgaacttg
    20701 cacgcgcatc aggtcagcat cttgggcacg gcgattctgt ttgactctgg atcatgggcg
    20761 cgtctgccgc cgtcgctgcc cgagtccgtg gcgctctcgg cgctggatgt cgccggggcc
    20821 gcgccgcaag tgacacggct gtgtcacgat ccacggccag gcaagcgggt cgagcgcgtc
    20881 ctggttctcg gcgcgtgcgg aaaatcggga cttctcgtcg gagccgccgc gcgaaaggcc
    20941 ggcgtaaaaa ccatcatcgg cgtcgaaagt tacgccaagg ccgccagcga agccgaatct
    21001 ctcggcatct acgaccaaat tgtccgcaaa gacgccgccg atgccctaga aattgcgacg
    21061 gaagtttgtg caggcggcga gttcgacctg gtcattagct gcgtcagcgc cccccgcgcc
    21121 gaaatggccg ccatcatgtg ctgtcgccaa ggcggcactg tctatttctt ttcgatggcg
    21181 accagcttca ccgccgccgc gctcggtgcc gaaggcatta gccgcgacgt cgacatgctc
    21241 attggcaacg gctactgcat tggacacgcc ggcgaaaccc tcgaactgct tggctcgcac
    21301 gcaccgctgc gcgagctgtt catccgccgt tattcctgat ccgtcgctgg ctgcgccccc
    21361 agccgatgca ccatctcgac caatgcttcg acgtgggccg gcggaatttc cggcgtcagt
    21421 ccatgcccca agttgaagat gtagcgacga cgcggcgcct cgcggagcac ctgttttacc
    21481 cgcgactcaa gctgcgcaag cggccccaag agcaaaagcg gctccaggtt gccctgaagt
    21541 ccctggttcg acgaaagcgc cgctttggcc accgacaagg gctgccgcca atcaatcgag
    21601 acgacgtccg cagacagctt gcccacctgc ggcaaaagcg cgagtccacc gagcggaaaa
    21661 tagatcagcg gaacccccaa aggccgcagc gccgtcagga ttttttcgac gtaaggcgcg
    21721 ccaaactccg cgtagtcttc cgtcgacaac accccaatcc acgaatcgaa tagctgcacc
    21781 gcctgcgcgc ccgccagcac ttgcccggtc aggtgcgcaa tcagcgattc cgtcagcttg
    21841 ttgagcagcc gatgcgcaag cgcgggctcg cagtacagca gccgcttggt cttctggaac
    21901 gtcttgcccg tctgtccttc gacggcatac gtaagcagcg taaacggcgc accggcaaaa
    21961 ccaatcagcg gaacaacgtc ggaaagctcg cggcgcaaaa tccgcaccgt ttccagcact
    22021 tccggcagca catcgggcga aagcacccgc agcgcatcga tcgccgccgc atccgcaagc
    22081 ggctggagcc gcggcccttc atcgccaaac gtaagcggca tccccatcgc gcgcagcggc
    22141 aagaggatgt ccgaaaacaa gatcgccgca tcgacgccca agcgatgcac cggctggagc
    22201 gtcacttcga ccgccaagtc cggcgtcgta caaagcgcca gaaaatcccc agcctttcga
    22261 cgggttgctt gatattccgg cagataacgt cccgcctgcc gcatcagcca aatcggaacc
    22321 gtgtctaccg gatcgcccgc gcaggcggcc aaaaagcgat tctttcgggt acaagcgaaa
    22381 gtagccatcg ccggcattat cgaagacagc ccagcgcgac gccgcacaat ttgtgaaaat
    22441 ttccacaggt tccgtcgcca ccacacttca cgttacgaaa gagccagcgc aagtgcgtgt
    22501 ttttgcagcc tttttcgacg gacaatgccc gacaggggcc gacagaaaaa cacgaaaaat
    22561 tacaatttgg tgacaatcgt tacccgacgg tgtcgcaaaa gtaccggcat cacgagccat
    22621 tcactcgcaa aacggatgga accatgtcgg gtctaacaag ctactttgcg tcgacactca
    22681 gctcctacga tcaaattttg atcacagcgt acctgctcgt cttcgtcgga cttgcggtgt
    22741 acggcttcca ccgctcgcag ctggtctact tgtactatcg ctaccgcgac cagcgccccg
    22801 aaccgctcga tcgtctgcgc gatctgccga tggtcacggt ccagcttccg ctgtttaacg
    22861 aaatgtatgt ggcacagcgg ctgctcgatg ccgtcgccca gattcgttac cctcgcgacc
    22921 gcttcgagat tcaagttctc gacgacagca cggacgaaac ccaggaactt tgcaagcgca
    22981 aggtcgccga gatcgccgcc caaggcatcc aaatcagcta cgtccaccgc gtcgatcgca
    23041 cgggcttcaa ggccggcgcg ctcgaaaatg gactcaagac cgccaagggt gaatttctgc
    23101 tggtcttcga cgccgacttc atccccgagc ccgacgttct cgagcgcacc atccacttct
    23161 tttccgaccc caaaatcgcg atggttcagg tccgctggga tcacctaaac cgcgactact
    23221 cggcgctcac cgaagttcag gcgctcatgc tcgacgggca ctttgtcatc gagcacaccg
    23281 cgcgtcaccg ctcgggccgc ttcttcaact tcaatggcac cggcggcatc tggcgtcgcg
    23341 ccgcgatttc tgacgcgggc ggctggcagc acgacaccct gaccgaagac atggacctgt
    23401 cgtaccgcgc gcagcttcgc ggctggcgct ttgtctacct gcccgacgtg gtctcgcccg
    23461 ccgaactccc cgtcgaaatg aacagcttca aaagccaaca gttccgctgg gccaagggtt
    23521 cgatccaggt cgccaaaaag ctgctgccga cgatcatgcg ctccgacgta ccgctgtcgg
    23581 tcaagactga ggcgtttttc cacctgacca acaactttgc ctacccgctc ttgctgctgc
    23641 tgtcgctgct gcttttgccg aacctgctcg tgcggacgac ccacggcctg cgcgaagtgc
    23701 tgcttatcga cctgccgctg ttcttcggaa ccacgctgtc gattgccgcc ttctacatca
    23761 ccagccagcg tgaaatcgat cgtggcggct ggatgcgcac catcaagcgg ctgccgctca
    23821 tgatgtcgct cggcatcggc ttgtgtatca accagacccg cgccgtcgtg gaagcgcttc
    23881 tgcagcgcga ctcgggcgaa tttgtccgca cgcccaagca cggtgtcgtc gccaaaatcg
    23941 aagcgtggcg cagcaaaaag taccgcgccg acaagaccct catcccggcg ctcgaggtcg
    24001 gcttcgcggc gtattttgct ctcgcgattg tgctggcgat gaaaaacggc cactggatgt
    24061 cgctgccgtt cctgcttttg ttcttcctcg gctacgccta cgtcggtctg ctcagcctcg
    24121 tgcagcttcg ctaagcccag cgttcccgcc gcgcccacct ccgcgtccac caagtcccgc
    24181 tcgccatacc tcttgaccta ctgtcgccgt aaagccacgc tttaacacat gaaattgctt
    24241 gtgcttgcgg caggttacgc gacaagactt cgacccctta ccgaccacca agccaagccg
    24301 ctactgcccg tcgctggacg cccgatgatt gatcacgtcc tcgacaagtt tgcggcctgc
    24361 cccgaaattg acggcatcta cgtcgtcaca aaccgtcgct acgcaccaaa ctttgtgcgc
    24421 tgggcggaag acctcggcgg acagtttgcc ggcaagccga ttcacatcgt cgacgatggc
    24481 acaaccagca atgacgaccg gctcggcgca ataggtgaca ttggctatgt cctgcgcacc
    24541 tgcaacatcg acgatgactt ggtggtcgtc gcgggtgaca acctgttttc cggcacgctc
    24601 gaagactttg taaaaaccgc caagcagcgc ggcatcctcg tcggtgtgta cgatgtggga
    24661 gaccttgagc agatccgcaa atacaacaac ctcgggcttg acgatgaagg ccgcatcacc
    24721 cacttcgaag aaaagccgca gaccccggtg agcacagtga ctgcgattgc gctgtaccac
    24781 tatccgcgcg ccgtcctgcc gtggctgcat cgctatctgg ccgagggaaa aaatcccgac
    24841 caacccggtc gtctcgtcga gtggctgttt ccccagatcc cttgctacac ctatcgcatc
    24901 acgggaatct ggctcgacat tggcagccgc gaaacctacg acgaggccca gcgcctgttc
    24961 caaacccccc aagggagcaa ggcatgacca tgacacgcca aacgtcgacg ctactttttc
    25021 tttccctttc gttgccgctt tggctggctg gctgcgcact gtccgaaaat ggtccggaaa
    25081 ccagcccgcc cgatctgacg ccaccgccgc agcgaggcat cgtgcgaatc ggcagcgcat
    25141 cggtcatgtc gggtccggat acctatgaac agtcgatggc gctggcgctg ttttacgatc
    25201 cgaatgccgc cagtcccgtc tgtacgcgca gtgaaacccg caactgtgta acccacaggt
    25261 gtggggcgat aaaaaacgtc ccgtcggcgg gcgacatcac catcaagggc ggcagtgaag
    25321 acgtgctgct gcggccaggg acaagcggcg cgtacaccga ataccgcaac acttcgacgc
    25381 tgacgtttca gcgcggccaa atcctgaacc tttcggccag cggcaaagac gtccccgcgt
    25441 ggcagacgcc gctgaccatc ccaacgcagg tgttctcgct ccagtcgccg agccctgcgc
    25501 gcatcgacct gacttgggtg ctgcacaaga cccaagacct ggccctgacg tggacaccga
    25561 ccacgccggg acattcggtt cggatcgagc tgtcgcagga ctttgggcaa agccaaggca
    25621 cgctgatcga gtgtgtgttc gacggcagcc aaggcgcggg atccgtcccc gccagcgtca
    25681 tcgcccagct tcagaccacg gtcggaatca accatgccgt cggtgttttg gttggacctg
    25741 gcagtgaaat ccggctcagt ccaccgggat gggatgtcag cgtgtggacg attggcgtcg
    25801 gtcgaaccgg catcgcaacc atcgcggact agcgaactcg ttgaagggaa agcggcgacc
    25861 cgtgtcgatc gccgcggcag gctggcaggt tagatgcggg caaacgccgc gtcgggaatc
    25921 tcgaccgggc tcttgtcttc ccgcgccagg atgtcggctt tgtcgatcac gcccggcagc
    25981 acgttgcgcg caaagtactg cgccgcgtgt cgcttgccga cgtagaacgc ttggtccgga
    26041 tggtccttct ccaagccagc aagcttgctc tcggccagca ccgcttgatc cagcagcagc
    26101 caggccagcg tgttttcgct catcatctcc aggaagcggt tggcgacaag cggcaccagc
    26161 tctagctgcc cgccgtagaa ccacgacaaa agccgcatcg tcgtgccttg cagcgccgcc
    26221 gtcgcttctc ccaggagctt gacctcgggg cccacgttcg gatgctcgct gtgacttccg
    26281 acgaacgctt ggatgtcggc cagatacgct tgcaggttcg cgccgcccgc catgcccagc
    26341 ttgcgaccga ccagatccat cgcctggatg tggtttgtgc cttcgtagat cgaaaaaatc
    26401 ttggcgtcgc ggcagtactg ctcgatcccg taatcgctgg tgtaaccgac accgccgagc
    26461 gtctgaatcg ccgtttcgca gatgcggaac gcctggtcgc tgccatacgc tttgacaagt
    26521 ggcgtgagca gctccacctg gccttggtga tagctggctt tgccgtcgcc accgccgtgc
    26581 gccgcaatcg tcgcccgatc gttgtgcgcc gccactttcg caatcagcgc ccgcagaccc
    26641 tcgacgcgcg acttcatgtc cagcagcatc cgtcgcacat ccgcgtgctc gatgatcggc
    26701 acccgcggcg cattcggatc tttgaagttc tggatcgagc tgccttgctt ccgctcccgc
    26761 gtatagcgca gtgcgttaag gtacgccgtc gaagccacag ccaagctctg aatgccgacg
    26821 ccaatgcgcg caaagttcat catgttgaac atttgcttca tgccttggtg ttcgctcgcc
    26881 ggaccgccga ccatttcgcc taggcaggcg ctgttttccc cgaaattcag cactgccgtc
    26941 gccgaagccc ggatgcccat cttgtgctcc aggcccccca ccgccacgtc gttcgattcg
    27001 ccaagcgtcc catcgggatt tacccgcacc ttgggcacga tgaacagcga cagacctttg
    27061 gtccctgccg gcgcattttc cgtccgcgcc agcaccaaat ggatgatgtt ttcggtcagg
    27121 tcgtgatcgc ccgacgaaat gaaaatcttg gtgcccgaaa tcagataccg cccatcgggc
    27181 tgcttgaccg ctttggtgcg cgccgatccg acgtccgatc cagcttgcgg ttccgtcagg
    27241 cacatcgtcc ccgcccactt gccaccatac agccgcggca ggtacagcgc tttttgttcc
    27301 tgcgtaccaa acttctcaat cacctcagcg accccgatag atagaccggg atacatcgca
    27361 aacgacgtat tggcacccgc caccagttct tccaccgcca tcccgagcag cgtcggcgcc
    27421 cccgatccac cccactctgg atggatcgac accagccgca gccccgagtc atagacccgc
    27481 ttccacgcat cctgaaagcc tgccggcgtt ttgacttttc catcgacaag tcggcagcct
    27541 tcgacatcgg cagcgctgtt gagcggaccc acgaccgtgg tggcaaattg gtacgcttcg
    27601 tcgagcacca tgtcgacgcc gctgtcatcg aaggtaccga caaacggcgc gctcttcaga
    27661 agctcgccga ggccgcactg ttcaaacagc acgaactgga tctctcgaag atcggcaaga
    27721 tagtggtttt gtccttgtgc catggtcgtt ccttctttgt tggatactga tactatctag
    27781 tcagatacct tcactatcca attgccgcac cgcgttgtca attgtccgtc cgcagcatgg
    27841 tttcgctttt tccgacgcag acgggctctc gcggtgacac gacctctgtc tgaccttacg
    27901 cacggaagat acccaggcca tgaccacagc agccagcccg cgacgcccgc ccttgcgctc
    27961 ctcgcacacg ccgattggcg cgcagccggt gctcgacaga ccgcccagcg aactcaccat
    28021 ttccgaagtg gccgaccgca gtggtgtgcc gatcgcaacg atcaagttct acatccgcga
    28081 agggctcttg ccgcgcccgc gaactgccgg acgaaccgtc ggtcgctacg acagcgcgtt
    28141 tgtaaaccga ctggaactgg tccgcgagct gcgcaaccgc tggcgactgt ccctgcgtga
    28201 aatcgccgag ctgctgcaag aagcgggacc gctcgccacg ctcaatgaga tcgagctaca
    28261 gcttgcggcg cgcgaccggc tcgacgaagc gatcgatccc accaacgcag agccacccat
    28321 ctcggagctg cggctcgtcg agcgatcggg tctgccgatg gccgacatcc acgatctcac
    28381 ccgctacgga cttttgacgc cgatcaaggt cgacggcgcg acggtctacg ccgaacgaga
    28441 cgcccgcatc gccgaagtcg tcggggcgct gcgcagctct gggtataatg atcacctggg
    28501 ctttggcgtc gccgatctcc agcgctacgt cgaggccctg cgcgtgctgg tcgtccgcga
    28561 agtcgaacag ttcgacagcc ctgccctgcg cggactcgga cgcgacgaaa tgctgcggct
    28621 gattggccaa ggcgtcgccc aggtggacgt gctgctcggc cttttgcaca aaaaactgct
    28681 gctggctgcc gtccacgatc ggctcgacgc cgcggcatcg cgccgccaaa ccgaaggcaa
    28741 accacaaaag cctgcccgac ggagcaaagc accacgctaa gcgaaatcag gagcgccttg
    28801 gcgtgaaggc aaaaaaaatg ccccgacggg cagcctaacg cttggggggg cgaacgcttc
    28861 ggctgaacgg cggggcaatt cggaacctat cacaacgaat ttcgctggca acaaaaaaat
    28921 tcgggaagtt tgcttctcga tgcacagccg gcccaaaaga ccccgccgac cgaggaactt
    28981 gctgcgcagc ttggcaaaaa ctaccatggc caggtgacgc tgcgccgcac agtgctgggc
    29041 ctctttgtcg cgcttatggc cattccagcg ccggcgctgg cctatcccta ttattcgctg
    29101 cgtccggtgg acttcgtgct cgccggcccc accgatggac acctggcgtc gctgtttttc
    29161 aatccggcgg cgattcgcgt ccagccaggc agccaggtgc tggtccaggg cggcgtcagt
    29221 ggtttcatcg gcagctatca gcgtcaagca gcgctgccaa gcggcttttc cccaggcggc
    29281 accggcagtc cggcccaaac agtgccgatt cgctgggcca acccgcaagc gctcgcggcc
    29341 ctcgcctggg atctgcgctc ggactcggtc acgctcgggc tggcgttttc gaccccagtg
    29401 ctcgacctga caaacttccg ccgcgacgat gccccgatag agcagctatc cacccgttac
    29461 cacgccatct acgaagagag ctacagcctg tggggctcgc tcgcgatcgg tctcaagctc
    29521 aagagctggc tgctccttgg ggccagcttc aattttggct acacccacag cagcttgtcg
    29581 tttttgcgcg atggcgatgc caaagcgcgc gactcgctcg gctgcggcgg cggcccgtgc
    29641 gaacagtggg caagtcgcac caacatcgaa atggatgtcg gggcacttgg ctacggcttt
    29701 tccgctggcg ccatcgtcga accccttctg gatcggctgt ggctcggcct ttcgtacctg
    29761 agcccgctgt ttagcagcca aggtgacttc gttgcgctcg aaggccagcc gtctgcgctg
    29821 tcttgggacg acggcagcgg ctgctcggga agctggaagg gagccaaaat caccgtcgga
    29881 aaccaggcgt cacagtgcgg tgctgcccgt ctgatggtcg cctatcccca catcatttac
    29941 tttgggggcc ggctgcgctt tccgctgtcg cccgccgtcg aaaaatccgc cgacagcgtt
    30001 cgcccgcgcg ccgtcgagct ttcgagctgg gcgcggctta cgattccagg gcacaccgat
    30061 caagaaatcc aagtcgagcg ccgactctta gaaccaggtc tttacacgcg accgctatca
    30121 gcccagaccg cattcgcggt aacattggga ataagacagt actttcagcg ggttaccttg
    30181 ggtcaagagt tgctttacga aacccctcgc gcggcagcgg gtacggtttc accagccaac
    30241 atcgagggtc acaagctcga cctttcgctg tcgttgcgcg cggtgcttca caaacggctg
    30301 tcgctgctgc tttcggttgg cgcgacctgg atgttcttcg cgcccgaaac aggtcaaagc
    30361 tacgatccgt cgctggtcga gttgtgccgc cagagcggtt acgacgtgac aagctcggcc
    30421 tgcgacgcag tgcagcatgg tctggccatc cccaccgcgg cagcaagcta cacccttggt
    30481 atccctcacg gaagcgccgg tcttgagatc aacctgtgag gacctcgacg atgaaccccc
    30541 agcctgttcg gacccggact cttcgccgcg tggcgccact tgttttgttg ctgctctcga
    30601 ctgcttcgtg cagtagcaca aaagacaacg ggagcagctg ccgcacggtg gccaactgca
    30661 cgatcgacac cgcgcccatc tgcgatgcgg catcgcttat gtgccgggcc tgcacgatgg
    30721 gtgccgatga catcgcctgc aaaaatggcc gtcccagcac gccgcgctgc ggtccccagg
    30781 gaagctgcgt cgcctgccta aaccacggtg actgcgccac caaggatctg cgaaagccgg
    30841 cctgccacaa ctttgcctgc ggcccgtgca aaagcgccac cgagtgtcag agcaagctgt
    30901 gcagcgccga tgggagctgc gcgccggttg ccgaagtttt gtacgtcgac aacaaaaacg
    30961 gcagctgcac cggaatcgat cacaagggca gcatcgacga tccgttctgc acgctgcgtg
    31021 acgcagtgac ggcggccaca atgggcggca agacgctcat ttccgttgcc ccatcgagca
    31081 agccctacgc cgcgctcagc gtaagtcccg ccgatcagct cacgagcttg cagattgtcg
    31141 gcagcggcac cctgccttcc gaaacgcgca tcgaaggcct gctaaacgaa tcagccatcg
    31201 ccgtgatggg cgacggcacg atgggaaaac cgaccgttca gctccgcaat cttgagctac
    31261 gcggcggaag cagcgccagt gccagcggcg tgtcctgcac ccaaggcggt gctgtgacga
    31321 tgagcaacat ccgcgttcgc ggcagcggcg gcagtggcat aagcgcgtcc aactgcaaca
    31381 tcatcgccga ccaaagccaa gtcttcgaca accgcgcaag cggcatctcg ctcaccagca
    31441 gcacgttcgc gctgtccaac ctgatgctgt ggagcaacag cgtttccggc atctcgcttg
    31501 gcgtcggcaa cagcggcacc atgcggttcc tgaccctgtc ggtcaacggc aaccccgcca
    31561 gtgctcagcc tgccggcatc gactgcggag caacggcgca cttggtcgaa cactcgattg
    31621 tctttgacaa ctactcgcgc gcaagcggca tcaacagcac cgacaaccag ttggttggct
    31681 gcatgctcac aaacgtcgtc acaaacgaca agcaagcgac cggcgggatc tacaaaaccg
    31741 cgctcgattt tgtaaatccc gtcgccgatg tctcaaacat cgacctgcac ctggtcaaag
    31801 acagccccaa aaaccgcgat gcctgcgtcg acaaggttgc acagccgcca agcgatcatg
    31861 acatcgacgg aacggcgcgc ccccagggaa gtgctgccga catcggtgcg cacgaagtgc
    31921 agtagccggc cccaaaaacc aaaaagccac cgcgttcttt cacagaaccg gtggcttggc
    31981 ggagcgggag acgggattcg aacccgcgac cttcagcttg ggaagctaac actctaccgc
    32041 tgagttactc ccgcgagaca gtctgtgtat ctactgcgcc cgcggcccgc tcgtcaacta
    32101 ataaaaccgg tgccgggggg cgcggacctg gcgggcttcg gcatcgcgcg ggcgacgagc
    32161 cgcgagcaaa acgtgatacg ctaaccagcc atggcagaaa gtcgcagccc acgcgccccg
    32221 gtccgcaaaa gcggcaccat gcgcattgaa accgtgggac gcgatcgttc tgtgtaccgc
    32281 accaccgcgc tggtggtggc ggttgcgctc ggtggagccg cggtcggcag tctgggttcg
    32341 cagctcttgg cgcgactcac cctgcgcaaa gccggccccg ccacgacaag caaagcacca
    32401 ctgctgttgc taaggtccga aacgctcgaa ggaagactgg cagagatcga ccaagcgctc
    32461 gcgcagcacg attcctcgat ggcgctcttg ctgtgtgaag aagcgctccg ccaaaatccg
    32521 tccgatccgc agcttctttt gcgccgcaag cgcgcagaag aggaacagct cgatcgcttc
    32581 cgtttcgaga tgctggatca agcgatttcg cggcggaact ttgccgcagc ggtcgcgctc
    32641 gccgaagaaa tctctgctgg ctcccccctc cgtgcgcaag cacaaacccg aatccagagc
    32701 gtcgtggccc cctacgtaaa gagccttctt gatgaagcag agcgcgcggt caagctgtcg
    32761 ctgtgcagtg aagcgcgcac gatcttgtcg caggtgctcc agctcgatcc acagaacagc
    32821 cgcgcccaag aaatcctgtc cgcttgtccg accaactaga ccgcgtttct gcgtcgtgag
    32881 tgtccatccc agcgcaccaa aggatggacg ctgggtacac atttactccg acgcagtgcg
    32941 tcgacgagtt ttctcattca gacgaggact tccgtagtaa catggcctcc tctttggggg
    33001 acgaccccga gccaaatcat gactcactcc ctgggaggag gtccgaatgt cgagcgcatt
    33061 tgccaacgta aaccgctact tcaccaaagc tgcccaagta ctcaatctct cggcggaaac
    33121 tgccgagcaa ctgatgactc ccttccgcga gctgcgcgtc gagtgcagca tccgcatgga
    33181 tgacggaaaa atccgcacgt tcgtcggcta ccgcgtccag cacgacaact cacgcgggcc
    33241 attcaagggc ggtctccgct acagccccga cgtcgacatt gacgaggtac gcgcgctcgc
    33301 ctctcttatg acctggaaga ccgcagtggt cggaattccg tttggtggcg ccaaaggcgg
    33361 aatctgcgtc gatccgtcgc agctctcgcc cggcgaactc gagcgactga cccgccgctt
    33421 cgtccaaggc attcaccttg tcatcggcga caacgtcgac attcccgccc ccgacgtcaa
    33481 caccaacgcc caggtgatgg cgtggttcat ggacgaatac aacaagttcc atggcttcca
    33541 ccctggtgtc gtcactggca agcccgtcga tatgttcggt tcgctgggcc gtgatgaagc
    33601 caccggacgc ggcgtgctca tcgccaccga agagtatctg cgcacccaag acaaaaaaat
    33661 ctcggacgca acctttgtcg tccagggttt tggcaacgtc ggcagccact cggcgcggct
    33721 cattcatgaa cgcggcggca aagtcgtcgc catcggcgat cacaccgcgt catattacga
    33781 cccggaaggg attcatattc ccgctgcact cgcctatgtg aaggaaaaga aagtcctcgc
    33841 gggctggacc ggcgcgccca gcattccccg cgacgaaatt ctcttccacg aatgcgacgt
    33901 actgatcccg gcggcgctcg gtggtgtcat caccggtgaa aatgcaggcc gtatcaagtg
    33961 ccgcgtcatc atcgaaggtg ccaacggtcc gacgactccg gaagccgatg acatcctgca
    34021 cgcccgcggc gttgtgattg tgcccgacat ctacgccaat gccggcggcg tcaccgtcag
    34081 ttatttcgag tgggtgcaga acatccagca gttttactgg gaagagctgc gcgtccgcac
    34141 cgagctggaa aagatcatgc gtcgcgcttt ccaggaactt tacgccaccg cccagagcca
    34201 ccgcatcgat ctgcggaccg cagcgttcgt cgtcgcgatc caaaaggtcg ccaaagccac
    34261 cgcccagcgc ggcatctagt ccggcgtcct ttccttcgca gccagcgcta ccgcgtaaac
    34321 acgaagtcgg tgttttgcgc ggcggtatgg cagccgttgc agattcctcc catcacgtcc
    34381 gcaccgccac gcaccatgat ggttccgctg gcgcttaata caaaccactc ccagcctttg
    34441 gcgcctggat tgaagtcacc gccgcgcttc gccatcgcgg tgccaccaat catcgcgccg
    34501 cctttggtgt gcgctttgac caggatggtt ccgatcggaa actgtccatc gctgccccgg
    34561 ttcgcgttgt tgtttatgta catcgatctc gtaatggtcg cgtcggtggc gctgtgtgct
    34621 tgacccagta cagatgccga gggtcccccg ccttgccgcg gtgctgtggt ctgtgcccag
    34681 ctggtaaatc cgacaaagtc tgcatcctgc gcctcgaaag tcgaactgcc gcaaccgctc
    34741 attgcgacca gtacacacag tgccattccc atgtttgcgt tcataagtcc cccacacgca
    34801 cgaaggagcc ccaacctatt ttgccaagac gcaaggggtt ccggatttgc gtccaccgct
    34861 tgagtcttgc caaaccggaa gtcgttacag ccaatcgacc aaggaatcgc tcccgatgaa
    34921 aaggcgatga ccctgcgctt tcttgccttt gctgatcggc tcgcatagcg tgtcggaacg
    34981 cactgccacg tcgatgctgg atgtccatga tgcccctcgg ttttttgcga atgtgtcagg
    35041 ccaaagatgg cggttgatcc ttcgcgtcag caacgatcag cacccgcaga tgaggaactg
    35101 attcctcggc tgtgccaagg tgacgccgaa gcctatgtcc tgtttgtgcg gcgctatcac
    35161 ggacggctga tgcgactggc ccgtagcttc gtcgcatcac aggcatcggc agaagaggtc
    35221 gtgcaagaga catggctcgc ggtgctacaa ggtatcgaac gtttcgaaca ccgcagcagc
    35281 gtcaaagcct ggctgtttcg gatcctgata aaccgagcca aaacgcgggg tcagcgggaa
    35341 ggacggaccc ggctgttcgc cgatctgacg atcgagctgc cacaggaacc ggatgaactc
    35401 ttggcgcagt ttgccccaaa tggtcactgg ctgtctccac ccgccgagtg ggaccacagc
    35461 acaccagaga ctcagcttat gagtcaggaa accaagcgag tgctggaaca ggcggttcgt
    35521 gatctcccgc cgactccacg ggcggtgatt ctgctccgtg acatcgaagg actcgatgcc
    35581 gaagaagttt gccaacagct ctcgctgtcc gaagccaacc agcgggtgat cctgcaccgc
    35641 gctcgcaccg cgattcgcct agcgatcacc aagcacatgc aaaaatccta accgttagtg
    35701 tttttgcacc gaatgaaact gctttagcag gcctgactcg atttcaggtg gcaacggttc
    35761 ttcggaaagc tgtcccaaga gcgcgatcgt cgtgcgcatc tgctgaaggt agcgtcggca
    35821 gcccaggcaa atccccaggt gaagccagaa ttgcagccgc tgcccgaaag tcagacggcc
    35881 ttctaggtag tcggtcgcta cgcttgtcag atcgcggcag ctccacatca ctcttttcat
    35941 catgattgga caacttgtcg ctcgcaaatt ccatgtaaca aaatcgctgt tccgtggact
    36001 ttgcctctgg agacgcggtt tgtccgtggc cgaaaaacgc ggcaagcatg cagtcgccag
    36061 tccaagaccc agggcattgc ccgaaccttc aaggagagtt cccatgaaca agaaagtgct
    36121 cgttgccgtc atcggttcac tcagcatcgc agccccggcc ctgtcgcatg ccgacgatgc
    36181 caagccggcc aaagcggaaa aagcgccgaa aaagggtgac aaaaaaggcc acgacaccaa
    36241 gggtggcgaa aagagctgcg gcggcgacaa gagctgtggc ggcggcaaga gctgcggcgg
    36301 caaaggcaag taatcgactg gcctgcaaaa accgcgagtt cgtgccccgg acccgcggtg
    36361 ccgtgattcc gattccaaac catctttttt cgaggtctct atgcgcattc gctcgcagtt
    36421 catcggtacg gccctgctcg cgctgtccgt gcttttccct caccaggcgg aagccaagaa
    36481 gttcgaactc gacggcaacc acagcttttt cgcgttcacc gcatcgaccg tgatctttga
    36541 tgtggaaggc aagttcacca aatacaagct agaggccagc ggcgatccag acaccggggc
    36601 ggatggaaaa atccggctcg aaatcgatgt gaagtcgatc gacactggca acaagacccg
    36661 tgacaaacac cttctaagcg acgacttttt tgcctccgcc aaatttccca agctggtctt
    36721 cacctcgaaa aaagttgcaa tcgagggcga caagctggtg gtcgatggaa cgctcgatct
    36781 gcatggcgtt caaaaagacc tcaagttgac ctttgccaag tcgagcggcg taaacggtgc
    36841 gggcgtcaaa gagtgggcct acaaggctga gctaccgatc aatcgcaaag actttgggat
    36901 cggcgcggac agcgtcgcag ccaagatcag tctcaaagat caagtcaccc tgcgcattct
    36961 ggtcgccggc ttccccgaat aagcttcgcc gacccagctg ggcagggcgc aaaaaaccac
    37021 gctaagctat ctgtgcgcaa aggggtaagt cacatcttgc gcggggccat ggggggacag
    37081 catgcgacgg gacattggcg cgcatcagat tgaagtcgac ggcgatctgg tgatcgcccg
    37141 atcttacgga cgctggacgt tgcaagaaac cgagcagatg atcgccatct ggaacgagct
    37201 cctcgaaaag tacgggcggc tgtttgaagt cggcgacttt tccgatggga tcgaactcga
    37261 tgccgcggtg cggcgcctgc tcatcgactg gtcaaagacc caccagttca cggcgctcgc
    37321 catcgtcgta aaaagtctgc cggtgcgcgc catcgcgacg ctcgtggtgc gcggggccca
    37381 gcttgtgaat cccggcgccc ccagtccgct gttctgttcg acgtttgaag aaggctacgc
    37441 ttttgtcgaa caggcacgca aacagagaag ctagctacgc cgaccacgtc gcagaaaaag
    37501 acccaagacg gactttgtct gcgacgctgt gccttgcgaa aactagttgt acagaaggtc
    37561 gaacgtcacc gaaattggca tcgtctgtcc gtctgcgagc gtgaacttgt tggtaaacgg
    37621 cagcgtcacc ggatcacccg tcaccggatc gcggcttgcc ggcgtgctct tgtccccgat
    37681 gtcaaagaag cccagaaacg tgtacgtctg cgcggcaagc ggcttggtgg tgatgctcgc
    37741 cgtgctcagc ttgtctgtct tcagatcaat cggcagctca acactcgccg ccgccatcgc
    37801 gcccttgcgt ggcccagtga tcgcgacgtc ttcgctgagg aacaagtctc cgtatacggt
    37861 tcccttcacc gtgctgtctt tgggaatctg accgcgcacg aagtcggaca gcttgaaccg
    37921 aatcacgaac ttggtttcca ccttgggaac catcatctgc tcctccggaa ccggatcgtc
    37981 cttcggcgca caagcccccg ccaaaagcag aagcgatagt ggaagtgcaa caagggcccg
    38041 cattacttgt cacccttgca gagccacggt tcctgactgc ccagcaagac accaaaggtc
    38101 gtgcacatct cgtgagggtg gctcaatgac tctttggtgt cgttgaacca tgtgcattcc
    38161 acccgaagta ccgatccttt gggtagatcc atggggtttt ttgtcatcag aagcgtcggg
    38221 ggatcgtcgc gatagtgagc gatccactcg tccactcgat acagttcctt catggtgtca
    38281 gccggcgtcg ctgcatacga caggacaaac ttcttgcccc actcatgcat gtgaccgcca
    38341 aacaagatcg atttgtagtc ccgcggcaca acgcagtcat aacttcgcgt cgcttgctcc
    38401 cccggcttga tttcaaactt ctccaggtag gaaccagctg ccgcggccca cgtcgtaacc
    38461 ttggcagcat cgacgcgacg gaagcgcaac aaatcacggg tccgaatcgg atctttcccc
    38521 gtgttcacgt aatgcgactg catcacgatt ggggtcccct tccgcatcat gaatgcctga
    38581 ttcgctgaca gctcaaaact gggcatggtc aggatatcaa atttggccat gtcctctggt
    38641 tttgagcagt cctctgatgt gcccggtgga agtggattct tcgctttgag gatcacgaag
    38701 tgatggccaa attttccctg gagcgtatca gccaggtcat aagccagatc ctcgccctga
    38761 tacagcaggt gcgtacacgt catcgagtct tcgcctgact tgatgatttt ttctgcaccg
    38821 acgaaatcga tgaacttggg atcggcagcc ggcatcggaa cgcgcacgtc gacggcggtg
    38881 cgttctagca tctcttcctc tgtttccttg ggggaacacg cgagcgaaaa gactgccagg
    38941 gaagacaggc agagcaggga atggagacgg gtcatgggcg gactcctttg gaaaaaggaa
    39001 acgggctagt tgtttttcgc gccagccaaa atccacgcgc gcattttctc gatctcttcc
    39061 ggttcgagag cctgcgcggg gggcattcgc tgaccgaact tgggtttgtc gcttgtcagc
    39121 ttttccatca gaaaagaggc atccggatcg cccggaacca cccgcatctg gcctggtgaa
    39181 agcgtcgact tcgcattcac gagctgatcg taagtcgaag caccgaggct caggccgccc
    39241 gccggagttc cggtgctctg atgacatgtt gcaaagctac agctgcgctg aaacacgtct
    39301 cgttcaatct gtcccagcga tggttccggt gaacaaccag agagcagcag cacactgccg
    39361 accactccgg acagaagacc aaaagcaaaa ccaactgagc ctgccgcacg acgttgcgtc
    39421 atcgcgagtg cctccggaat gggaaatttg cagcgcagac gtagcgcgca cagcgttccc
    39481 tgtcaagcac ttctgtcgtc ggaaagtggc agcaagaagg tcaagaatgg tcgaaaccgc
    39541 ggaatccgcc cagccccgaa gcccagtcgg ttagtgactc tctggcgcga tgagtccggg
    39601 cttttcgatt ccgtgcagcg cttcgaccac tgaagtccgc accgcaagca gcggcgccgg
    39661 caaaagtccg cccgccagca aaagaccaaa cagcgacacc gccacccagc gctcgcgcgg
    39721 cagcaagtca ggaaaaccac cgcccgatgc cacctcgcct gttgcttcgc ccaggaacac
    39781 ccgttggaag gccagaaaca gcacgattcc gttgagcgca gtggttagga gcaccatcac
    39841 cgccgcaatt ccatggtggt cgagaagtcc ctgcacgatc agatcctcag agacaaagcc
    39901 gagcgtgccc ggactcccga ctgccgcaac cgccaaaagc agaaagccgg tcgccatccg
    39961 cggtgccgtc cggacaagtc cccccagctt gcgaatgtcc gccgtcccag cacgcgccac
    40021 aatcgatccg atcaaaagcg caagaccggt tcgcaccacc accgtgccga tggcgtgaat
    40081 cagcgcgccg ctgacccctg gcgcattgac cgacgcgatg cccgcgagca aaaagccctg
    40141 ctggctgatc cagaaatagc caagcatccg ccgcaagtcc gtctgtgcca gcgccagcac
    40201 cgcgccatag cttgccgtca aaagtcccag cggcaaaagc agtggcatcg cctgcgcaaa
    40261 cagctctgga aacagcggca tcgtcacccg cgtggtcaaa aacatgccca gcggcgtcgc
    40321 aaacgcggcc agcgtaagcg gagccggacc gcgctcggac agcggaacga tccaaacgtg
    40381 aaatggaaaa cagcccatcc ggaccagcgc cccacacagc gccaagatcc ccagccagtt
    40441 gggaagcggt tcgctcatct gccgctgtcc gagcgcaagc agatcatagg gcgtctgcac
    40501 cgccgccccg ctcttggccc gcaaaacgcc gagcatcacc acaaacacca ccatcggcag
    40561 cgagctgcat acgcccaaga tggtcagcat gcggaccgcg ccccgttccc cgcgcttgtg
    40621 cagcgaccac aaaagcggca caagcgacgt gacccaaaac agcgtcagga tcgaaagatc
    40681 cagcgagatg agcacaccaa ggagcaagct ctcggtcagc agcacttcgc ggatcaggga
    40741 agccgtcagc aagcgccgcg gcgccgcaca caaaaccgcc agcacaatgc ccgccgacac
    40801 cggcaccagc accgcactta ggccatcaat gccgacgtga tagcgaattc ccaaaaacac
    40861 cggcgcccag gctgcttccg tgcgggcaaa tgcgtgatcc gatgcgtcac gcgcaaacgc
    40921 ccacagagac aaaagcacca ccaaccccga cgtaaaaatc cccagcttgc gcagcgcgcc
    40981 atccgatgaa aacacacgac cgacaagcga cgccacaagt ggcacaagcc agatcagcgt
    41041 catcagccat agatcagttt gcatggccat cctccttgct cgccccgtct cccgatagcc
    41101 atgtcaccac ggcgttctcg acccgaccca aagcccgcgc cgctgcgagc attgggcgcg
    41161 ccacccagcg atccagcacc gattcgatgg caaagcgctc gatggccagg cgatacaaaa
    41221 atcgcttgag cgcggacgac tcaccaccgg ctgtttgcgg aacactgcca ccggcccgcc
    41281 gccgcgccag cgcatcttgc agcgtcgatg gtgtccgcaa aaactggtag tagcgcaaaa
    41341 gcgagtgcgc cgccaaatgg atgagcgcca gccgcggcat tcccagcgcc acttcgacaa
    41401 acatcaaacc cacctggctg atcgtcgcgt aggccaggcc gctcttggcg tccgccgaaa
    41461 cttgtccaga aagcgcagcc atcactgccg tcacgccgcc gattcccaga atccaaagcc
    41521 gcgccagggg cgcttgttcc aaaagcggcg ccgaccggat gagcaagtac acgcccatat
    41581 gcaccgacaa tccgccgtaa aacaccgcgc tcgatgccgt cggaccttcc atcgcccgtg
    41641 gcagccagcc gcccaccgga atctgcgccg acttgcccat cgccgccacc agcatgcaga
    41701 tgccaataaa aaccgccggc ccgccgtgtg acgccatcgc caggtgtgga aacaccgtgt
    41761 ggtattcggt gctgtgcagc cagccgtgca gtgccaccgc cccgaccaag agcccgatgt
    41821 cacagagccg gtacgtcaca agcacccgca gcgccgcccg caccggcccc gatcggtcct
    41881 gaaaaaagcc gaccagcagc accgatgtcc agccgacaat ttcccagccg gcaagcagca
    41941 ggtcatagct gccaccgagc accagcaaca tcacgcccgc agcaaacatc agcatcagcg
    42001 caaaaaagcg cagaaagccc ggctcgcgat gcaggtagcg ctgggcaaaa cgactggtgg
    42061 ccaagatcag caccgccgcc accatcgaca ccacgacact ggccaggtca aagtacagtc
    42121 gcagttccag cgcgtagtca ccgctgcggt agatatcaat cacccgcaag tcgaccggct
    42181 ttccgtcctg cgaaatccac atcacggaac cggtcagcac cgacgccagc gtcagccaca
    42241 gcgaaaggtg cgtgatcgcc accaccaggt tttcgcgcgg acggttttgc aaaagtgcca
    42301 gaccgccaac caagagcgcg cctgccgccg gccatgccgg agccagcgct gcaaacgacc
    42361 agattgcttc gctcgaaatc atcctgcacc tccgacccga aaccgccgct tgcgccgctt
    42421 ttccccaact tgcggggccg cgtcgcgcga caacagcgcc ggaggcaaaa agctgcgatg
    42481 cccctggtag tagtcatgcc acgtagcggt cttcgggatg gcgtccgccg cgggcggctc
    42541 accacttgtc ggtcgccacg gaacaaagcc ctcgccggga acaaactcca gcatgtcacc
    42601 ggtatcgggg tcgaccgata tgacgtggac ccagccgttt aacacaaact cggccacccc
    42661 tttttgccgt tccagaatgc tcgccagcac cgccggacga ttttcaatca ccaagtgcag
    42721 ccgcaccggt tcatggattt cgatcatctg agacggcaag cccgttcgca gatcgctgca
    42781 cgcgccgttc atgacgccaa acaggccggt gacgttgtgt ggcagcttgg tccctgcgcc
    42841 cagccgctcg ttgtccatcg tcgaaaagaa gtattccagg ttgatgcccg ccccgaccgg
    42901 tgtcaccgcg gccagcgtgc gttccagcac cgtcccgttt ggatcaatcg tggggtcgta
    42961 ggaaatcaaa aacgcccgcc gatccagaaa caacccttgg gtcagcgccc gtcgaccgac
    43021 gattgccgaa gcattggtcg catgcccaag ctcgggtctg gcttggctta ggtccgcact
    43081 gcgttcatcg acgtgctcaa gcgcaagctc gggggaaatc ccaagcggtg ccgcatcaaa
    43141 acggcgcgac cgctcgcgcg cattttccgt gagtgcccgc tgcatgatcc gcatctgttc
    43201 cgcaagcaac agacggtgcg attctggcag cgcgtcttca tcaaagacct tgatggtatc
    43261 gtcgcaggtg tcgtgcacgg caccgacgaa acgggtgtca tcgggaatgt cgatgccacg
    43321 cttgcgcaga agctcgcgca cgggctgtcg gttggccatc cgcgcaaaca gacgcgcatt
    43381 gggtccaccg ctgcgaccgc cacacgcgcc acaggcatac gccgcaaagt gcgggttgtt
    43441 cacgctgtgc gagtcatggc ccaagatgac caccagacgc gcaaaacgcc gagtcagtcc
    43501 gatgttttcc agcagcgtgc ccacacggtc cgccttttcc ggaatcgcaa acccggtctg
    43561 catcccctcg ccacctgtcg agccgtcttc gcgaaccgaa agatgggtgc gaacctcggg
    43621 aagaagctga cgactgaccc gccgccgcat ccgcgccgcc actttcgggg caaagatcgt
    43681 caatagcagc ggcaccgccg accaaagccc gctcactgcg gtcaccagcg gacccagcca
    43741 aaacgatcgc gaagcgactt tgtacgtttc tgaaacacgc ttccagcggg cttgtcgccg
    43801 ctgccgagtc gcccagccct cggcatcttg ttcgtcgggt tcttcgacaa tcttgtgctg
    43861 gggcgtcacc accaccggac acagcggaaa cgtcgatggg tcgtcgatcc cttggtaggc
    43921 aatcgccagg ttgaaaaatc ccgctgttcc aaaggtctcg atctgcgccg atgccgattc
    43981 caagtgccga cgcagcgact cctcgcgatc gtcgatgcag cagaccacct gcaaaagcgg
    44041 cggctcgatc ggcgtaaccg caccactttt ggcgcgcgcg gcccgattgg ccacaagtcc
    44101 cacaagcagc tcgtctcgat agtgccgctc atacgccttt tgccacagct cctggcgcgc
    44161 cgcggatgaa aactcaaaaa gccaatctac aagctgctgc gtctctcccg ccccgagcga
    44221 gcgcagatcc tggggtcgca gcccggccag ttgtccaagc tgaaacagcg aaaacgccag
    44281 cggtccgcgc ggctcctcgg tcttctcttc gacgacccgc tcccgcagcc acggcaaaag
    44341 ctggttcatc ggccccgaaa agccacagcg ctggcccaga tccaaaaacg ccatcgcatc
    44401 gagctccagc cgaacggcca gaaaatcaat cagccgcacc tgcgcacgct ggctgatgat
    44461 cgggtcgctg ctctgctcca gtcgcgcaaa cattccggcc cagcccgcca ggtgcagaag
    44521 cgtctgcgtc acatactctt cgatctcgtc gtcggcaatt cccagctcat cgagcagcga
    44581 caacacttcg tgctcggcgt catatccttc gcggcggtgg gcacgaatgc gccggcccag
    44641 gccactgctc cacgccggac gaaccaccaa accggcggtc atgatctctt cccagaccag
    44701 atagaagccg tgcttgcggt caggcatcgt ccactgcgct tccccgcgat cgaggaacgc
    44761 cgccgccagc ggaatcaagg tctgatgaac cggctcgcag gggtcttcac cgctcagcat
    44821 ttgcagcgct tcacggaaaa agagcgggcc gcgcggcttc gccgctggaa gctgcgcacg
    44881 ctcggcaatc tcttggcagg tctgccacag cgcaaacacg atggcggctt ccgtttcggg
    44941 actgctcagc accgagtccg cgttttgcac cgatagcaag cgcttgcgag cggcttcggg
    45001 aacgtcgtca cgcagccgcg acagtgccaa ggtttcgcca agcagccagc ccagcccagc
    45061 cggcgtcgtt tccggcagac cgtgcagcat gacaagaagc gccagggttt ctttcgcggg
    45121 cagccgctgc ggccaggact cctgccgcgc tgccagcttg gattgggcaa gtacaaagcg
    45181 cagatccgcc tgtgaaatcc gcccccgcgc cagcgcttgc cgataggtcg cctcgggcag
    45241 aaacatccgc gcccgcagcc gctgtcccgc gactttcagc gcatcaaaga agggcagcga
    45301 ctggaacgca tgcagcgtgt tgtgatggac aaacacctcg agcggcgcct gcgctggcaa
    45361 gtaatgagcg gaatgcgaaa gcgcgtgtcg caaaatctgc tgacgactgt ttccactatg
    45421 gtgtggtgac gcttcaccat ctgtcacggc aatctcccca atgcccagcc caaaaggacc
    45481 tagccatttt cgtaagttac agtctgtggc gtcaccgcag caatgctgct tgcagccaca
    45541 aaaaaccaac gacaatgcgg gagctgtggt ggctctgggg cccacgggcc caaacggcgt
    45601 acagatgtcc cgatttacgt tggctcccgc aaccggggaa tgggcgggcc gcgcagcggc
    45661 gaattgagca agcgcaaggc ccgcagcggc agtgcatcac cgctgctgca cagccgaatg
    45721 ttcgtccacg ccggccaaag cctgggagac cacgacacac cgcgatggac cgccgagcga
    45781 tgcgcatccg ccccaccccg cgggctggca tcgtcatcgt ctgattcaac cgtcgcgcca
    45841 tcttcgaccg gggcgccatg gctgcgtgac gagtcaccgg gcaggtacga cgcagtctgc
    45901 tgattggtcg gccaggcact ttccgccgcc ccgctggcct gcaaaagcag caggagcgga
    45961 agcagaagcc agagccgagc cagcagcaac ctcatctgcg cccatcctaa cagccatcac
    46021 acgcggcgca agctggcgtg attgtttgcg tctggaactt agggggtaag aaaagcgctt
    46081 cagccgccgg ccagggccag aagtcgcacc tggatctttt cggccacaat ttctgccgcc
    46141 cgcagcttgt cggatgtcgt cacgtagggc accacctcgc tcgaactatc cagcacttca
    46201 ccatcttgca gtgacgtcat gcgcgtcacg acccggagca gcttgccctg tttttcaaac
    46261 gagccttgca gcaccacttc cgcccccaaa atcttgccaa gcttcgctgc cgccgacttg
    46321 tcgatgtatt cctctttgct gcgcaaaagc tccacgaggt tctgttcatc aaactggttg
    46381 cgctcgagca gtttcactcg cttgcgcaac gccgggctgt tggcaaacgt gtccatcagc
    46441 acttcgccaa ttccttccac cagcaccgca agctctcggt cggcagccag gtttttgaac
    46501 cgcaggacgg ccacccgcag cggcccttgg tgatgccgca caatcgactc tggcagcggg
    46561 ttcgacggcg actgcgcgcc cacttgcgac ggtgcatctg gtgctgtcgt ttgtccgggt
    46621 gccacaggcg gcgtaggtcc ttgtgtcggc gtcgcctgat tcaccggggg accgctcggg
    46681 ggtggtgagg tcggcggctc gggaggttcg acatactggc gcgacgcttc cgccataggc
    46741 cgcggtcccc ttcgccccga cttgctcatc gccagtccca tcaccaccac agccacagcg
    46801 ccgagagccg ccgccgccac cagcaaaggt cgcgataccg aggatcgccc gcggtagccc
    46861 gactgcaccg tcgtccccga gacgatctca tgggctatca gcgcagcttc gcgcgccacc
    46921 tcaccgcagg tctggacgcg cgcctcgggc cgtttttccg tcatccgttc gatcaagcgc
    46981 cgcagcgacg ccggaacacc gggggcttca tcgcgcaggt caggcagcgg ttcattgatc
    47041 tgtttcatcg ccaccgccac cgcggactgc ccggtaaaag gtggcttgcc gaccagcaag
    47101 tgaaagccca caagacccag cgcataaata tcggcgcggg catcggcaga ctgtcccgac
    47161 aaaacttccg gagccatgta gtgcagcgtc cccggcgtcg cctgcgtcga cgtcagctca
    47221 tcggtcgatg ccatgtcgcg ggcaatgcca aagtcgacga tttttacgtt gccaccgtcg
    47281 tccagcatca agttgtgagg ctttacgtca cgatgcacaa tcccgaccgc gtgtgcggcc
    47341 tgcagtccgt cggcagcttg ccgcagcaca tccgcgactt tggcgtttgg cagcggtccc
    47401 ttttccttca gcagactgtc cagcgagcgt cccgatagaa gctccatgac aaaatacgga
    47461 ttgccttgct cgtcccgccc aaagtcatag acctgcacca cattgggatg ggatagcttg
    47521 gccactgcac gcgcttcccg ttcaaagcgg gccaaaaact ccggattttg gcccagcatc
    47581 actttgatcg cgacggtgcg ctgcagcgaa gggtcgtagg ctttgtagac agcccccatt
    47641 ccgccctgcc caagcagttt tagctccgtg taacgaccaa gctgggttgg caacgtagcg
    47701 ctctggctca tcgaagattc ctcaccgcaa gcgaggcaga agtctagcat gagcgacttg
    47761 ggcttgcgcg ctgccgacgg aagacatcgg ccagaaaaac ggtcttggac ttgtgcaaag
    47821 cagattcggg acaggaaacc acttggcgga acgacagctc acaatatgaa tcatctgaaa
    47881 tgcttcatat tacaagcgac ccacgccaag cggcttgtcg gcggctattc ggacagatag
    47941 acgcggggca gaaagacacc gtggcgtgcg atggtaagct gccgcgcctg gctcgctgta
    48001 tccgccatgc tgcgcagcac agaagccagg ctgtccggcg tcacaggctg cccatcggaa
    48061 ctcaccacca gatcacccac ttttagatcc agccggtcgg ccaggctgtg ctttgccacc
    48121 gcggtgattc gacaaggcga gtgtccagcg aacgcaacgg tttcacagct tgcacccacc
    48181 ccgtagcgac tggcgacacg ctgaagccca cgcgggtctg tatgaaacac atgggacgcg
    48241 gtctcttggg cggcgcgatc gaactcgtgc tcgatgatcc ggacgcctac gttttcccca
    48301 gcgcgcaccg tctgtggttt gcccgtcacg gtgtccagga tgtcgatttc gttttcgcgc
    48361 accgcgacaa gcttcaggct gcggccaatg gtctgcccca cggtcacatt gctggtttcc
    48421 caggtttttg tgtcggccaa ggtggcgaac gcctcgctgc cctcgccttg cgtaccgaac
    48481 agacggaatt ccgccgcccg agcctggtat tcgctggcct tggcaccggg ttcagccacc
    48541 gcaagtgccg caacttcgcg atcactgacg gtcgatccgg atgatacatc ggcccccgtg
    48601 caggaagcca gaagcggcag cgtaacaaac agtagcgaaa caatctgcgt cttcatggtc
    48661 ggcctccttc tactttagac agctggggac gttggcaagg gtgccgtatt cggcggcttc
    48721 ccacgcagca caggcaagct tgcgctggcc ggtgtctttg gcgggatgga agcagtcaag
    48781 cccgtcgatg tcatccttgc caaagtggta ggtgcccacc gatgagttgc tcgtggcatt
    48841 gccgcgccag tcggtgatga agttcacttt gccgctgtat ttggtcgcag ccgcatcaac
    48901 ttcggcacgc agcccgtcat tgtactgatc gacgcgggcc ccgatggcgg cacgacggcc
    48961 tgcgttgctt tccccggtga caacgcggca gacgccagcg gtcgaccaca ccaagttgca
    49021 ggtcagaaaa ttgcgtgcgt tgcccgcttc atacagatag tcgacgcgcg gcatcgacag
    49081 cacctgcacc tgagagcccg ccggcagaca gccccccaac agttcaagcg ccgccttcag
    49141 cgcgttgcga taggtcgtga ccgagtagag attggaagtc gcatcgctcg acgagccttt
    49201 gtcccgcgag cagatgtcgt tgccacccag ctccaagacg acgcggttcg gtttttgcgc
    49261 catcgcgcag atgcgggcag cttggccggc ggcattgttt ctgcccccga ccatctctgc
    49321 gccgctttcc gagacaaact ctttggtcat cgcggaattt ttggccttgt agcgcaggta
    49381 caaagaattg accttggcat cggtgccctg gccaaagccg tattcgggat gctcacccgg
    49441 ccagttgttc gcaccccagc cttgggaaat gctgtcgccg atcccggcca gcttggcagg
    49501 gtcggcagcc agaagctgcg cagggacaag tagcggcaaa aggacgccca gacagacagt
    49561 caatcgcatg gagaacctcc ttgcccaacc gccttggcgg tgggcgaccg aaggacgatg
    49621 caatatgcaa ctgacagacc accgcttgca ccaggtcgcg gacacataac aagcccgacg
    49681 ctggcgcggc ccaagcggag gcaccaaggg agcacaaccg ctgggaagca aagaccccaa
    49741 cttgctgctg tctgtaggca tcaaccgcca tgcgctggcg ctgccacttc ccacagacca
    49801 gacatttcgc ggctcggctc ggtttgtttg gctgcaaaca cttggtttcc gggcggggtc
    49861 tgtatcacta tgcgcggcat gaatgtgctc gtcaccggcg gctgcggatt catcggcgcc
    49921 aactttgtac agcagcagcg acggctgtat ccgcaagatc ggctgattgt gctggatgcg
    49981 ctgacgtact gcggcaacct gcgcaacttg gaaccacttt caggcgatcg cggcctgcgg
    50041 tttgtaaaag gtgatatctg cgatggtgac ttggtcggca agctcctgct cgaagaggag
    50101 atcgatgcca tctgtcactt tgccgccgaa agtcatgttg accgatcgat cctggggccg
    50161 ctgccgtttg tgcagaccaa tgtggtgggc accacggtgc tgctcgatgc ggcgcgaaat
    50221 gcaggcatag ggcgctttgt ccacgtatcg accgacgagg tctacggaac cctttcgcct
    50281 tccgaccccc cgtttacaga aaacacaccg cttgcgccgc gctcgccgta tgcagccagc
    50341 aaagcggcat ctgatcacct ggccttggcc tatgcgcata cctacggaat gccggtgatt
    50401 gtgacccgct gctcgaacaa ctatgggccg tatcagtttc ccgaaaagct gatcccgctg
    50461 atgattttgt cggcgatggc agacaagccc ctgcccgtct acggggatgg tcagcaggtc
    50521 cgcgactgga tctatgtggc ggaccactgc gcaggaattg atgcagcgct gcgccgcggc
    50581 cagccgggcg aggtctacaa cttcgggggg cgcgccgagt gcagaaacct gcaaatggtc
    50641 cactcgatct tggcgctgct tcacaaaccg cagtcgctga ttcgttttgt cgccgatcgt
    50701 ccgggtcatg atcgtcgcta cgccatcgac agccagaagg ccgaacagca gcttggctgg
    50761 caagcacgct ggacgcttgc ggacggactg gttgaaacgg ttcgctggta tcgcgaaaac
    50821 cccgcgtggt gccaaagcgt ccagtccggt gaataccgca cgtttttcga acgcaactac
    50881 gccgatcgac ttgtcaccgc caaggaggtc gcctgatgcg cgtcgcggta cttggtccgc
    50941 aaggcagctt gggcgtccat ctgtgtgagg agctgcggca cgccgggcat gacgtcgatc
    51001 cgatcgatcg gagcatttgt gatctacgca atcccgatcg agaaaacctg tatcggctgc
    51061 gggatcgact gaccgaacac gactgccagt tggtggtgaa ctgcgcagcg tacacggacg
    51121 tcgatggtgc agagcaaaac accgatgaag cgtttcagat aaacgcgctc ggcgccgaaa
    51181 tggtggctcg ggcagccggt gaagcgagcg cagcgctgtg ccacatttcg accgactttg
    51241 tgttcgatgg cacgcagagc cgcgcctacg atgaattcga cgaaccacgg cccctatcag
    51301 cctacgcgcg cagcaagctg gcgggtgaac ggctcgtgca gaagtctgga gtccgcagct
    51361 tcatcgtccg cgtgggcggt ctgtacggaa gacgtggccg caactttgta tcgacactgg
    51421 tggcacggct aaaagtgaac cagccgctgc gcattgataa gcagcggctc accgccccaa
    51481 cctggaatcg gctgctgtct cgacagctca tcacactctg cgagcgagga gagcatggcg
    51541 tgtaccacgc gacgtgtctt ggtgaaacga cgtggtacgg ctttgcgcag gcgctgtgtg
    51601 aagaagccgc ggcgctcgga tgcgagctgc cccaaacgtt cattggtgtg acaaccgatg
    51661 aactggcggc accagcagcg cggccagcat cttccctgct cgactgccgg atgctgcgac
    51721 tgcgcggtct tatgcggctg ccgtcctggc gcgacgcact caaaggatat ctgaccgagc
    51781 ttttgatcca gggagagctg taaaaatcag cgctttcccg gcatcagtcc aagctgcttc
    51841 atgcgccgcc acagagtcac gcggctcatc ccaaggagcc cggcggcctg cccaacatga
    51901 cctgcggcgg cccgtaaggc agtcagtacc cgctcccttt catcgacagc gtaacctcgg
    51961 tgggtcatca cactgccaac cggcacagga accggacgcg ggtacgaagg cggtggaacc
    52021 ccgagcagct cgggtggcag gtcggacact tcaagtactg gtccttcgcc aatcacaaac
    52081 gcatattcga tcacgttgcg cagctctcgg atgttgccgg gccagtcgtg cgcggtcagc
    52141 acctgcgacg ccacatcgct gatccgatcg atctgccgag atgagcgctg gtttagttcg
    52201 gcgatgaagt gatgtgcaag caacagcaca tccccggtcc gttcgcgcag cggaggaata
    52261 aagatgggaa ccacgcgcag ccgatacatc aagtcggcgc gaaaccgtcc cgcctccact
    52321 tctccgcgca gcgccttgtg ggtggccgca atgatccgaa catcgaccga gatcggttcc
    52381 tgcccaccca cgggaatcac gctgtgggtt tccagcacgc gcaaaagctt ggcttgtact
    52441 tccagcggca tctctgccac ttcgtccaga aacagagagc ctttgttggc cagtcgaaag
    52501 tggccaggat tgtcgcgcac cgcccccgtg aaggccccgc gcacatggcc aaacagctcg
    52561 ctttccaaaa gactcgccgg caacgctgcg cagcttatcg catgaaacgg tccatgctga
    52621 cggggcgaca gatcgtgaag cgcgcgcgca agcagctcct tgcccgtgcc agtttccccg
    52681 cgcagaagca cactcgcttg gctctgggcc gctttttcaa caatccgaaa cacactttct
    52741 aagccgggat cttgcgtaac catcccgaaa aaagtggccc gcttgctggt gggtggagcg
    52801 ctgtgcttca tggaaagcgc ctcgcagagt gcaccagttt gcatcgcttg tcatcgaatc
    52861 gttcggggta acaaaaaaca gcgccgccgc tgcgcggaat ttttccgaag catgcacacc
    52921 gcgcggccaa gcatcgaaag acaagttacg catccactgg ccctgcggac ccagaggctt
    52981 tgcacaattt ggaaccaggc cgcagcatcg gccacct
//
LOCUS       DAORGN010000011        48523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_239_length_48523_cov_6.647541, whole genome shotgun sequence.
ACCESSION   DAORGN010000011 DAORGN010000000
VERSION     DAORGN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 48523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48523
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_48523_cov_6.647541"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKL17_02470"
     CDS             complement(<1..611)
                     /locus_tag="PKL17_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43618.1"
                     /translation="MPQSISGKTHHRRKQRQHQRRRRFVLLRPPLQPIQQTDFFGDDL
                     FTVEKAGQVVSQRLRRRIALFFVFGKTARTDDLQRLWQSRSDGAQKRRLARHHAHDQV
                     DDGVVVKRCPSRKHFKQRRAEAVHITATIQVASFPTRLLGRHKRGRTHHGARLCQTAA
                     FTRFPRQTKVHHHRHIRSIPPPFHHDVGRLQIPVDDAFFVGVM"
     gene            567..2732
                     /locus_tag="PKL17_02475"
     CDS             567..2732
                     /locus_tag="PKL17_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43619.1"
                     /translation="MFASVVGFAGYGLRQSYVARKQSELQRHIAQDSKDIEWMIRTAY
                     SLPLHNVEREQDKVRKRMDQVEYKLRTYGDLAAGLGHYALGRGHLSLHQHKKARQHLE
                     QARDLGEKGPELHAALGRVLGELYHEALLEARRSGEKTWVEKRQKELQAELLDPATLY
                     LQKALSRRHELSLDSPEYIDGLLDFYSGREQEADYKAQLALVQAPWLSEAAKLRGEIA
                     QAQALRKVSDGQHDQARTLLSQAIEHFRQASDISRSDGLISAALSRAYLDLADLDRLQ
                     GKPQEQDFEKAQAAAEKTITALPSDASGYLEKTRILYFKAKSIKIKGNKIEILNEAIQ
                     LADIAIGRDKNNFIAWDVLGNALTTRGIFEQRIGKDPITYYQKAEKILRHSIELAPNF
                     PWGHNDLGICFTQWGNYILDVGKDPREQYRSAIDSMKHAISLDSRYVFAYGSLVGALS
                     QFSDYSLNHGQSFDALMRSAEELTQQCGTGCSSYLTFQRNMAVLWINHGLHLLQRGDD
                     PTVAVSTARGFLARQRQLDKNMYSECLFSRYADFAEAGWALKQDRDPTGLANRALAAT
                     ELCKRFDDKAQEPFMKSARFHLIKAQWQLKKRQTASEAINQALADASQAVSLDAESVD
                     SWVEKARASYYLATTLQGSAQALRLGEALAAVERGLKVNGSAGPLWALHAVIRRAQAR
                     AEKTPDVAMRWRKESDESWNKALAINPLLKNDYGDLLGE"
     gene            complement(2793..3932)
                     /locus_tag="PKL17_02480"
     CDS             complement(2793..3932)
                     /locus_tag="PKL17_02480"
                     /inference="COORDINATES: protein motif:HMM:NF039450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HNK43620.1"
                     /translation="MKRSIFSMLCGLSLLAATGCQNANQATEQDSVQVKLKACQARDP
                     ETGACQDPNGSKIFNPEKPDYSFAIGYPDWSGFEYMVTGFYPEAGKWKVRGWSMSPDP
                     LAPDHHVKSDGQVEGMLVDGNLLKVKFMEARQTRLLITFCDATGTLCDTLDVNSMFDK
                     KLAMLLSIPNPTGRGVTMFQWTFTSGANPVEYVKAWGADVVGQYVNTAAKGQVPTSLC
                     KKTGGADEYVVFEQGYLWDPDTYGRTVDPLAITVACEEGAIAEGLARGYTPWSTGKLD
                     AGMEANMADFQQAFIRMKTADYCGIGKSHTLDGTKYLINAPIDPARDRDPMPNLEAIW
                     GPDGAFCVIPDNRRHKEITMACALPDCDAATVDARRNYNLMDSIP"
     gene            4355..5746
                     /locus_tag="PKL17_02485"
     CDS             4355..5746
                     /locus_tag="PKL17_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK43621.1"
                     /translation="MSDHASTLKEAVTPRSDEPLGVERNPGVALLFHAGTPCVLAIPL
                     HCGAVELGRGTHGMEPDQRMSRRHARISYDGRRFFVTDLGSHNGTSADGHALSASAPV
                     EVQRVVRMGDSLFGLFHDIAPLVQRGVVVSQGKVMGPLLQDVFALAKQSAQYGDTLHI
                     TGESGVGKEMVAQGFHQFGPRAGGPFVAVNCAAIPQGVAERLLFGTRRGAYSGADANA
                     DGYIQAASGGTLFLDEVGDLDLQVQAKLLRVIETKELLPLGANRPHRVELHICSATNK
                     DLKYLAAQGRFREDLYYRIARPCVNIPPLRKRIEEIPWLIDSAVKKTTTTAAIHASLV
                     ELCLLRPWPGNIRELLVEVRSAVQNAAIKSSTRIEGKHLPDGAGTVLRSLGAVADFVP
                     DDYTPPAVRASDPGELARIEQALRSHQGNVAGTARALGLHRTQLRRLIERHGIDPQRF
                     APDGTAHKLDLDK"
     gene            complement(5750..6778)
                     /locus_tag="PKL17_02490"
     CDS             complement(5750..6778)
                     /locus_tag="PKL17_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNK43622.1"
                     /translation="MRALVLSQTLSYCDSVPDPIPTEGEALVAVRQAGICNTDVELLR
                     GYMGFSGIPGHEFVGTLLEVPPGHVDEAGRPLETGCRVVAEINCSPPPSPPTAALRAH
                     DPCRTTLGIFRRNGAFAQYLTVPVQNLHRVPDEVSDDQAIFVEPLAAALQIPNQVHMA
                     PTDHVAVLGDGKLGLLCAQVLAHASAAKVTAIGKHPHKLALLSGHPLATRLLSQLGDD
                     AAARFDVVVDCTGSPSGLSQAIKILRPRGTLVLKSTYAPPSVVKDNANGQDGAAQFAA
                     ALTQIVIDEISVVGSRCGPFAAALRLLAQKRIIVDPMISARYALSDGLAAFADAMTKS
                     QLKVVLTP"
     gene            6849..8495
                     /locus_tag="PKL17_02495"
     CDS             6849..8495
                     /locus_tag="PKL17_02495"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNK43623.1"
                     /translation="MTHARSYGLESLDLLSTVLAKDDSGTPVCELRLDTQCMSLPTDF
                     VHRYGAALSRAHAAMLALESGAIANPDEKRMVGHYWLRDPSRAPNPDIAKEIEDCRKR
                     IVSFADAVHRGEVVSSTGQRFRQLLLIGIGGSALGPQLVAAALGGTADKLRPYFFDNT
                     DPDGFDRVLAQLATGDGGLSTTLVLVVSKSGGTKETRNGMLVAEAAFAHAGLPFARHT
                     VAITQDGSELDQTAQRAGFLATFPMWDFVGGRTSVLSAVGLLPAALQGIDIDGLLRGA
                     RMMDQATRLAFPEQNPAMKMAVCWFDATGGQGKRDLVVLPYKDRLELLSRYLQQLIME
                     SLGKEHDRDGRQVHQGIAVYGNKGATDQHAYVQQLRDGVDNFFVTFVEVLRDRSQPSP
                     LVPDSLFVEQQVSAGDYLLGFLLGTQLALFEKGRRSLTLTVTELSALTLGALIALFER
                     TVGYYAELVHVNAYHQPGVEAGKRAAASVLSLQKRLLAVVDDTPRTATELASRAEAFD
                     DDIVAFKILEHLAENGRLRRHRDADTAITAARYSRSLRLG"
     gene            8506..9441
                     /locus_tag="PKL17_02500"
     CDS             8506..9441
                     /locus_tag="PKL17_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK43624.1"
                     /translation="MTQDAPIFSSVADVSERLRSQGYIASPQIATSVLLAERLHKPLL
                     IEGPAGCGKTELAKVLSQTLGTDLFRLQCYEGLDEAKALYEWAYPKQLLYTQILRDKI
                     GDLLSGVGSLADAVAKIASHEDAFFSERFLLERPLLKALRAKRRVVLLIDEVDRAEAE
                     FEAFLLEVLSDFQVSIPELGTLSARHMPLVILTSNNTREMTDALRRRCLYLHIGFPDA
                     ARELQIVHSRLPQAPSQLVTDVVRVVQALRTLDLKKNPSIAETIEWVRALLVLGQTHL
                     EPQLAEETLNLVLKYQGDLDTAREQKKTLFPDAKR"
     gene            9463..10434
                     /locus_tag="PKL17_02505"
     CDS             9463..10434
                     /locus_tag="PKL17_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43625.1"
                     /translation="MRAVSPSPRYDAVADLWVIAAYYNPHGYRTRAHNYALFADSLRR
                     SHIPLLTVECAFGTEPFVLPDGPDVLKLRCDAVLWQKERLLNVAVERLPPTCTKVAWL
                     DADILFERADWATQTSLLLDEKQIVQVFSTVVRLPRGATSLPALPDGESPELIESFAS
                     VYGRDPSLHLGGHYHEHGHTGFGWAARRDLLLRTGLYDTALSGSGDHLIAHAAVGDHE
                     SKCIDKVLGKSTPHREHFRRYCDRFFARSRGQLAAVPGTILHLWHGDHEYRRYSDRNI
                     ELVKLGFDPARDLCLANSGAWEWGSKHPELHAWAKEYFARRKEDGDE"
     gene            10517..10738
                     /locus_tag="PKL17_02510"
     CDS             10517..10738
                     /locus_tag="PKL17_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43626.1"
                     /translation="MPVSPDAFASQALAARAKIVAALDEIHGILADCDEDEVATWMAQ
                     AQELRRALSIIDEQLQLFRSDPAQKDLRH"
     gene            10786..11622
                     /locus_tag="PKL17_02515"
     CDS             10786..11622
                     /locus_tag="PKL17_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNK43627.1"
                     /translation="MLLLRLLQKPDRAPRFGRVLDDEPEVVELLDGDPLLGGKPTGET
                     LAFAPAEWQADPDGSEIELLVPVVPQKIIGVGSNYRNHAKEMGKPIPSEPLLFLKPPS
                     ALLSPGRAIVRPQGFERCDYEGELAVVIGRRCHRQPVSTAMNYVLGYTLCNDVTVRDL
                     QKKDGQFTRAKGFDSFCPLGPVISTDIDLGRTAVRSHLNGKLVQDGQSDELIFSIEEL
                     VSFVSQVMTLEPGDVITTGTPAGVGNLQPGDVIELSADGIGSLRNPVIAGALLPSLPA
                     RS"
     gene            complement(11650..13962)
                     /gene="ppk1"
                     /locus_tag="PKL17_02520"
     CDS             complement(11650..13962)
                     /gene="ppk1"
                     /locus_tag="PKL17_02520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNK43628.1"
                     /translation="MTVPSTPSHPPLPSTGSPSSSSDSGLLDDPGLYLNRELSWLDFN
                     QRVLDEASDQSVPLLERIKFLSITCSNLDEFFMVRVAGTKQQIVSGLIETGADGLLPQ
                     DALARVAQRAQKMVADLYRLWHKDLQPRLHQAGIDLLSVGELEPEQRRFLYTYFNAQV
                     YPALTPLAVDPGHPFPNVRNKTLNLAILLQRGAQGLRGQEASFAVMQVPSVFSRLVEV
                     PTKGPRRVFVCLEDVISLHVADLFPGTRVLGCWPFRVLRNYDMTIDEEESMDLLQTIQ
                     KEVRRRDRQPAVRLDCHAGMDVAVRRFLERALELDSQDVYPVTGPLNLTDLMEVYAKG
                     QTSEARDLYDEPFVPKVPARLQDASTMFEAIAQGDILLHQPYESFESVVDFIEEAAED
                     PNVLAMKATLYRTSGNSPIVAALVRAAELGKQVTALVELKARFDEENNIVWARKLEEA
                     GVHVVYGLIGLKTHCKVALVVRREAGGIRRYVHLSTGNYNSQTAKIYTDLSFFTCREE
                     FGIDASALFNMLTGYSQPPRWHRFVVAPLGLRERILDHIEAEAQNARAGRPARIIAKM
                     NALSERDVMKALCRASQAGVQIDLMVRGVCCLRPGVPGLSENIRVTSIVDRFLEHSRI
                     FYFESDGKRDVYISSADFMNRNFQRRVEVMFPVEDIKLKERIVDEILAISLLDNQRAR
                     VLRRDGSYERIVPGPTDVPLRSQWRFVELAKAQATVPLIDRGLTIRPTMRLSARTAED
                     RPPATPPKTPTRTLLPPIPGSGDDKNPGGK"
     gene            14089..15579
                     /gene="guaB"
                     /locus_tag="PKL17_02525"
     CDS             14089..15579
                     /gene="guaB"
                     /locus_tag="PKL17_02525"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNK43629.1"
                     /translation="MSQQVDPILPLELSLSFDDVLLLPGHSSVLPTECDVSTKLARLP
                     LRIPMMSSAMDTVTEEQTAIAMALMGGIGVLHRNCTPDEQAKMVRRVKKFQHAVIASP
                     ITVRPSMTIGEVHRLIAETGITGFPVVDDAGVLVGMCTGRDIRYVTTPSMRVCDVMST
                     PARSLMLGATRSEAEDFFRRYKLEKLPLVDAQGRLGGLITSRDWREAKSHPDAVRDEQ
                     GSLMVAAAIGVGEEALSRAEAQVEAGVDMLVIDSAHGHSDGVLSTVRAIKQRFPSLLL
                     SAGNIATAEGAIALAEAGADIIKVGMGPGSICTTRVVSGVGVAQFSAVMEVTQARSRR
                     FPDVAIVADGGIRYSGDVTKALAAGAGAVMIGGLFAGTDEAPGELVLYQGRSYKTYRG
                     MGSLAAMKKGSKDRYGQGGVAESSKLVPEGVEARVPYRGPLGSVVAQLLGGLRSGMGY
                     VGAKDLGELRRRARFRRITAFGLRESHVHDVTITAEAPNYSGTFED"
     gene            15591..17114
                     /gene="guaA"
                     /locus_tag="PKL17_02530"
     CDS             15591..17114
                     /gene="guaA"
                     /locus_tag="PKL17_02530"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNK43630.1"
                     /translation="MLCVLDYGSQYTQLIARRLRSQGFFTQVLPGNLSPQAVAAYAPD
                     GIILSGSPRSVGTGFDPDPGLLSLGVPVLGLCFGYQFLAQHLGGRVAAATHREYGAAM
                     VSKTPAGRRDPLTAKLSQTTPVWMSHGDSVVDLPPSAELILESAGKPAGFVVPSRKLW
                     GLQFHPEVYHTPEGKLLLEAFARDICRLSPDWNLRAELGEIKAKLAKELHGVDEVVCA
                     VSGGVDSTVLAVLLSQVCKVRALFVDHGFLRAYDIRNLRRVFQHFPNISLEVVNAQER
                     FWAALDGVSDPETKRLEIGRLFVRTFAEHVGEKHFTHLAQGTIYSDVIESAAVAHGGA
                     AHIKSHHNVGGFPQDARVQLVEPLRPFFKDEVRELGVLLGIDSEILHQHPFPGPGLAI
                     RCIGELRRERIEILRRADEIFYEELVRRDMYLRTWQAFVALLPVRTVGVMGDQRSYEH
                     ALAIRAVSSVDAMTAEVTEFSLAELLPIASRLINEVRGINRVVYDLTSKPPGTIEWE"
     gene            17178..17471
                     /locus_tag="PKL17_02535"
     CDS             17178..17471
                     /locus_tag="PKL17_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43631.1"
                     /translation="MFAPQLALQSICETTPSLPGPELSLRQQEQKLRRQLRSSLRLGD
                     LVQQAACYLSLGDIELSRGDSERAGELYRRALKLSQTAHESRQAKARSSVSHK"
     gene            17517..17858
                     /locus_tag="PKL17_02540"
     CDS             17517..17858
                     /locus_tag="PKL17_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43632.1"
                     /translation="MSKPPTKWSFPRRTSYGVLERFLGQEINVFGPRQSDEHDNCLGG
                     GFLVSIEDGFLLLAERPSEAPDLAVALSEVVVIAVVKDRPELTAVDGGKIHRFRRPDA
                     PGPSDDDLDDN"
     gene            17921..19240
                     /locus_tag="PKL17_02545"
     CDS             17921..19240
                     /locus_tag="PKL17_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNK43633.1"
                     /translation="MTHTAWFRRGGFRSAVLFGALAVGLLYVPKAAADPKPSLRSRVF
                     GYIPYWTASKKTWDLRGLTDVALFSVGASDTGTLTSTTFWRGKDARAIIDDAHKQGIK
                     VELAVTNFDPTSLHNLLSSTSAVDKLVAALVEESLVTQPGDGLSIDFEGLRAADRAAL
                     VSFVTKLRAAMKAKRSDSQLSLATPAVDWSKAYDYPGLAAQSDTLFIMGYGYHWSGSS
                     TPGPGAPLSCGAPWGSICLAATVKDYVAALGDGLRHKIVLGLPLYGYDYPADSDKIGA
                     KALGTGTAVLYSAARKQVTNRRYEPVSQTPWYVYTDAKSVIHQVFYEDEESIGKKIDF
                     IVEQKLGGVGFWALGYEDDSLWMVVKDKLVVTDPPPPLPPPPPVVDPPMNPETGSVAE
                     AQGCALSSRSSHMPTFPALLGVGSATIFLLGKRRRRTVRYNRPARAV"
     gene            19314..20567
                     /locus_tag="PKL17_02550"
     CDS             19314..20567
                     /locus_tag="PKL17_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HNK43634.1"
                     /translation="MSEQSTPSSSLSVNAIAKLVITLLCSFVAIGLRLSHFHLPPLEG
                     LVANGTAVLSAAFILAWAAEALQVDIAKGLAMAVLAFIAVLPEYAVDLYFASMAAKHP
                     SYAQYAAANMTGSNRLLIGVGWSVVALCSVYAALRKRDPNAQKEPSPGVSLGDGYGVD
                     LMILAAATLWSLTMPLFEQIALWNGAGLLLLFGFYVWRVSGGADAEPELTGVAQELGS
                     LPKVRRRVTVALLLVAAGATLLAAAEPFAESLIATGEHFGVDRFLLVQWLAPLASEAP
                     ELLVATIFALHGHGAAGMGALLSSKVNQWTLLVGTLPMAYSFALGKPGALHLDPRQVE
                     ELVLTSSQSLLGVGVLLNGRLEAWEAVVLLILFLVQLPFPQASVRYGLSMLYIALAIV
                     LFLRYARYVPGHVRLLLRKESSDEG"
     gene            20574..21506
                     /locus_tag="PKL17_02555"
     CDS             20574..21506
                     /locus_tag="PKL17_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43635.1"
                     /translation="MLRVSAGLRLLLSPLHTLLEPTRDLLLRAQAALGDGQRGAMVRA
                     FVELHALLLSPLDALVEKRSGRGSDDSPGSALLGLIGLWDELPDIDVERENLIELLAQ
                     HAATPPAFDVPPPDGLAHHDGVSAAQRAWLDQVLSVRACPTPQWWRRLSRRARGPLGD
                     RARLTVERTQDATVRRIFEHLPRPGTDDDWTATTDLGELCAKDQLTLAVQVPLPGQIA
                     MLHLCGDSQTARLDVVLPETPSEAVVRRHLEIVELHGELATERLPDGSIADQALVVVW
                     VPEVMPPSWIVDMQLRFGVPPEARIWRYVYRVVG"
     gene            21628..22926
                     /locus_tag="PKL17_02560"
     CDS             21628..22926
                     /locus_tag="PKL17_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43636.1"
                     /translation="MLRSRHKPARLSLGSRSRSRPRSARSQQLFGAVGLRSERRSGQK
                     LWQKLRAPLQILGALAVLLVINLYVLYYRHGTSLPALLRQAETGRALSARLSGPVGTP
                     PQPTRPLRKPKVLPPLPDYPRVVEQPLHVSDSLGDVLNKVAVPARQKSELATALQGVL
                     DPGGFGPGQSLTFFYDHDERLQAVDYRLTDSLAYHLERVQTGSADRFVPVKQTQPLVR
                     STLRLELGFEGKNDLQAAMLRAGETPALAARVYEVLACELNLYSDTQAGDKLRLLVEK
                     QHLGDRFYRYGRLLAVEYLPATATTGGRNRRLRAFLRSEGSPVNAGAAPGASALYFGD
                     SGDSLSRSICRAPLLWSRANRTTLASAPQLQGEKGRYVLDYPAEATVPVVAIAAGKVK
                     LGPSRPGKPQTVVVQHAGGLETSYGPIGRLVRGLVDGQIV"
     gene            22936..23163
                     /locus_tag="PKL17_02565"
     CDS             22936..23163
                     /locus_tag="PKL17_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43637.1"
                     /translation="MIGYVATPAGRDAAHLRLQARLSGRVVDPGKLKSTREPGIPDAE
                     RAAFVAQSSDWAEKLARSDEAVIATRGGESR"
     gene            complement(23132..23986)
                     /locus_tag="PKL17_02570"
     CDS             complement(23132..23986)
                     /locus_tag="PKL17_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43638.1"
                     /translation="MATKPAEAATAPTAPKAPTNAPAKAKPARAKTVAAPATEPSEHA
                     LLPTETARPKRPVLPRVKDMLLRVPSEHVLPPIQPVPHMVSEAHALFSVAKRYADKLA
                     KIGFSQPMLDGLSLYAHALSEAQDDLDAHRGKQYAAAELALFHQASELRNQILLAARL
                     TLRKDRKVLESLDAIVEGEGLGDLARDLLDLALIAESYPAQLSRIGLGLETAHKAREL
                     SRKISTRGAPAKVPTADERVMEDLRDRAATVLHELMTEARLGGAYVFRKEPKLALLFT
                     ATHRRGSR"
     gene            24360..24755
                     /locus_tag="PKL17_02575"
     CDS             24360..24755
                     /locus_tag="PKL17_02575"
                     /inference="COORDINATES: protein motif:HMM:NF017719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK43639.1"
                     /translation="MAKGIEDVQSYLLKMDLRYEEPREGIWVLSGLDGIDKLVITLAG
                     PVLVFRVKVMELPQRNREGLYRTLLELNASEMMHGAYGVEGDAVVICDALQLENLDYN
                     EFAATIDDITLAVASHHSRLGKFKDPLPA"
     gene            24807..25601
                     /locus_tag="PKL17_02580"
     CDS             24807..25601
                     /locus_tag="PKL17_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNK43640.1"
                     /translation="MGFFSRLGSLIKSNLNDLISKAEDPEKMLNQVLVDMKTQLVEAK
                     KQVAVAIGDEKRLKKQWDEQVALSKDWERKAMLAVQAGDDELAKEALLRQREHEELST
                     QFEQQWVHQKDAVDKLKVQLRVLTNKIEEAKRKRNILIARQKRAEAQKAIQNTMAGLS
                     DTSAFDTFDRMAQKIDQLEAETEATTELAGELEGDSLTQRFKALEAKSTPADLALAEL
                     KQKMGLLPAASSAPPKALPQTSTTKPEAKPEAKPEAKPEGDPTLDP"
     gene            25651..26463
                     /locus_tag="PKL17_02585"
     CDS             25651..26463
                     /locus_tag="PKL17_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNK43641.1"
                     /translation="MNLFERPVSEYMSAPVCVVSADDTAEDANKIMSEKRISCLAVVG
                     RDGMPAGVVSRSDLLRVARAMVFATGSPTALQLPSMCVGDLMTPHFYSVTPKSTLIDA
                     AQMMVEKRIHRVFVVDGTKPVGVCSTKDLMRAVMDAKIQTPISAMMTVPVVTVESSSL
                     LSDAVATLSRARISGVAVMENGVPVGIFTQEEALAARDRPGQSPVDESMSQALLCLPV
                     TTPMFRAAGFTISTRARRVLAIDHHHVQGILTGLDFARCILPPPPTVEVKIA"
     gene            26492..27460
                     /locus_tag="PKL17_02590"
     CDS             26492..27460
                     /locus_tag="PKL17_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNK43642.1"
                     /translation="MKRSGVSIRAKMLGGLWGSVVGDALGVPVEFHSREERERDPVQE
                     VRGFGTYNQPPGTWSDDSSLMLCSLHSLLERGFDLADMGQRFVRYLTDSYMTPHGSVF
                     DVGIATATAIDKLRAGCIAEEAGGKGERDNGNGSLMRILPIALFFYRESASDLIARAH
                     LASRVTHGHPRSQLACGYACLFIARLLCGDSAAVAYERVNAQIPRLYSDSHWQSELPH
                     FARILSGRLAGEPKSTIRGSGYVVHTLEASVYLLLRSSTYSQTVLDAINLGEDTDTTA
                     CVAGGMAGVLYGEDAIPKDWLAPLARREHLDTWFRLFVDNVQPADS"
     gene            27545..28414
                     /locus_tag="PKL17_02595"
     CDS             27545..28414
                     /locus_tag="PKL17_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK43643.1"
                     /translation="MDAKNPREVLAYVPPSREKIRLLTAPLRHFFSPVFLDLEKLDLS
                     RPALWVGNHTLLGLTDVPLLFERLYVKHNVVLRSLGDRLHLKVPFWGDFLVSHGMVLG
                     DPEVGAELMRGQQHILVFPGGGREVMRRKGESYQLLWKQRLGFVRLAAQHGYDIIPFG
                     SVGADESYRILIDANDILAGRFRRQFLERSGLLTLLRGGDFIPPLVMGLGGTLIPRPQ
                     RYYFGFGARIPTAQLHGNVDDKQALWQIREKVAHAVRAQISALLEYRKTDRATWSPLR
                     RFLAPIDGNDTDS"
     gene            complement(28439..31672)
                     /locus_tag="PKL17_02600"
     CDS             complement(28439..31672)
                     /locus_tag="PKL17_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK43644.1"
                     /translation="MILCAVASPDLARAQLQNGQIKGVVTSDLNGKPLPGVTVVVTGP
                     ALQGEQIEITDGNGRYLITEIPSGDDYVVRFYLGDKVEERSGIRITHGKTLTVSFAFP
                     IKQVARETKVIRERAPNVDTASATAGVEINQEVLKNTAVRGRTFESVLAMAPGTVDPP
                     RGQGGDIGVSISGSTGNENNFIIDGLNTSDPNTGIIGTELHQYFIKEVNVITGGYQAE
                     YGRATGGVVSIITKGGGDELHGSVFLSMQPFQLTPKTVGRLGEALAYRRKADGLLGDI
                     GFELGGPIKKDKIWFYIGFAPTVAQYQTDRVIRHAIWNPQTGTARLNGGYACPTYLSD
                     PALCRGSAALATEMDEVSAQSFTELRQLYNGIAKLQFNLSPDHNLTLSYIASPKVFSG
                     YSYLGSGTSGNVEDSQYQQTDQVHDASLRYVGKLLGRKLQLNAMYGFHFQGLNVAPKN
                     VDKPLSLYLTESTNPYSLADFEDNPDCRRQMQRDLMGNAVSFNPCPITTYRTNGFGQY
                     NRTDLQRHQLLVAATYFLEALGTHAIKLGFDFEHLRNQNFRAYSGPNGDPSDGLSANV
                     MYRTSADGQRLRNFAQYSTVGPNGETILLNSFSSDTFTNNYVLYLRDSWNVGPVPGLV
                     LNAGVRWEAQELHGSDGSRQIGIYDNWAPRAGAVWDFTQKGLGKLFVNYGRFYQSIPL
                     TINDRQFAGEGVFTGAETTDCATAALQPGGRAVPVPRTNGTGTCNLSEIAAGNQNGGR
                     YGNVIPGLKGMYMDELVAGISYDVGWDLVLSASYIYRGLGNIVEDMSLDGGTNYIIAN
                     PGVDPDPSRLRDLQADVDRLRASGDSSQMGQEALGRAQARLDAYRSIATQYPKPRREY
                     QGLVLTAQKRLSNRFGLLANYTYSRMLGNYPGTYDGVVDENLPNFSSQFDLQDMMKNR
                     TGPLPNDRPHNVKIIGTYQQPIRKGNLTASLSFSAYSGRPINVLGTHFIYGDSQVFIL
                     PRGSGGRTPMVSQFDLHVGFEQPLSQKVNLSVYADVINLFNQRAVTNVDDDYTYSNVA
                     PIVDGKIADLAHLTDIDGHPIVKNANYGQPTGYQAPLYLRLGARLSF"
     gene            31934..34093
                     /locus_tag="PKL17_02605"
     CDS             31934..34093
                     /locus_tag="PKL17_02605"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNK43645.1"
                     /translation="MNKPSAIRSGTSRARWVRPLLFALSLLGADIAHAETSGFPMDSV
                     GLRVPGPLSSRSASYRLRARLDENTHEVKGQGTLLFRNVERKEAHELVFNLYQNAFKH
                     HATTFIREAGAQLRGDEMPEHGFGAIDVSSIKIGGVELIGKAQVLDTLLTVKLDTPVG
                     PGSIVEVELAFTTKLPKVFARSGYAGDFHAVTQWFPKLAVFECSRDGERCGFHAHQYH
                     GFTEFFSDHGSYEVTVDVPEKFVVGATGSQVAETRGQGRKQLTYRADDVRDFAWFADP
                     NFVESKARVQDGLGDVEVRLLNQPGLSSAVPRHFAGVRAALLEGEDRYFSYPYKQVTV
                     IVPPRDAGGSGGMEYPQLITSLLSPLPDGVRIIESVDAHEFGHQWVPMMIDTDEVEEA
                     WLDEGLNQSFTGLAMDRLFPEGCTSVKLLGFCLNDRDVDWLSTRSVLRRMPLVTPSFR
                     LSGRTYGAMTYAYTSAMLRSLANYLGPDRYRQALRHYAERYRFRKPKGPDFISAMSEG
                     AGEDLSWFFSQTVQTTRVADYEVMALENHKHELAFGLWDCPPKPPILPGHVADADRPD
                     WLRDINEANAAACAGKEPGRHELSPPSEKEQKKAKHDGKAGEKDSTDSVITIRRRGEL
                     IYPVDIVVTFEDGSRDTSVWTVAEQLAHPEERLKIMRFFRRAAVEKVEVDPQMKLMLD
                     EKRLNNGLSAKPNMRPVNRLFLTVQGLFQGLLELFSL"
     gene            34114..35052
                     /locus_tag="PKL17_02610"
     CDS             34114..35052
                     /locus_tag="PKL17_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43646.1"
                     /translation="MQANVSVQESGSSANAGTLSAFSSGFARVLSSPLLVVGMWLLSL
                     GTALFATLPVRAMLRDVLSLRPIADKIARGEYDVGLIEVMSDNPAAMAAVSSTLMTAA
                     LLYVLLQALVAGGVLSRLSPADAARHVPAGRLLVRSAETAGAMLKLELLFALAVRTPL
                     LLVAAALCGTVLGWQKVGSLAWDTVSARLIPVGLAFVMLWSLASSWLHLARLRRLDDE
                     SLSAWRALVNGGKTLSLSPVLSGALLLALLSVGGHGGLLWIGRLVTAKLDAKLLLLVA
                     FLVRQGLSLVRTTLSLWALSTTCSLDAAVSSRTCAR"
     gene            35154..35891
                     /locus_tag="PKL17_02615"
     CDS             35154..35891
                     /locus_tag="PKL17_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43647.1"
                     /translation="MKLSATTAVNKGTFVAAWLLASLALPESLSAQEPAAPFPAASPV
                     ASAPSGTAPGSIGSLDTASATPAASAAPSASTDKLSDLDRRTRELRDQVFRSKTRLTL
                     LTENLLGSAQGGAKLVVVQKDQMGRLFQMVKVSYQLDGREVFVRSLEPGRPADAKEQT
                     VYDGSVRPGDHTVGVAVVYRGDGNKVFSYYDQYTFTAKAAHRFSVTDGQTTRLEVVCR
                     EKGNPLLTRVEDRPSFDFRVESTKKAN"
     gene            35920..42150
                     /locus_tag="PKL17_02620"
     CDS             35920..42150
                     /locus_tag="PKL17_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK43648.1"
                     /translation="MARPWLRRRLLTLGFVGMFGLLLPQAAPSSAVATEQAAIPLPQA
                     LDQLDTALTSLESSVQAGQSTPAKTPPESAAPVATPQQMVGLAKQLHERRLFAAAASL
                     LSETLVRSRELSQDADVLWLLGDSLSELGDLRGAKRYLGQFVDSHPTDGKALTKLLAL
                     SSTLPADPMEKTWLGKLQAIPVASLPESANYVAGKLLFLRGDLAGAQKSLEAVVETSP
                     YFYRARYILGALAVQQQKLDGAETIFKSLTERPLPRRDDDVRVVELSHMALGRIAHER
                     GDSKRAHESYLHISQKSNLFKDAIYEASWAALKAGDSERAEQSLKLLLIAYRDEPQTV
                     LPVTEAKLLLGDLLLRRGEPDLASPYFVSARTDLRPLVEKLGGDLGTSPAFAERLRGQ
                     LGVAKAPLSLSNLAPQSLLFSLRGDEVLAQLQAMDRELYQTRVALAEQEELITRAEKQ
                     RTPQQQQQQSPGGTAELAEPRNRSLALYRYLVALHRAGGDRLDALLGPAATPEEKAQL
                     DGLSQKRAQVGKGLAEPRGGNDPLDGTASARRLHAELLGEAPSLYLHLLLRLSPPQRS
                     QADELLTNLNRMAQIERRLRDVIGKLDRMLDVRVGDMQAALQSERDALRGQRESLDKL
                     QAETAQVVGHAAWTRLQQVVSDYKELLVHAEAGVAEVAWAQRQKSSDELSQIQADQAR
                     ELRMLDDAFALPEDNRPAAKPAQSPQQSPQNQTAPNVPAMPASAMPAPAKSEAAVVAK
                     NEEIPLPADLPVELQADYRRYFEAARSYKHTLDEVGRDGYKVRRSHLKNEFAARLLRE
                     EAREKQLRSEAIVELENFLSRYPQHPRHSPDAMFRLAELLFERTSETFIGQSKSQSGE
                     MVALPDYTPSVDLYRRLLRDFPQYRNSHLATYLLGYCLGEMDHDEEARQAFLGLVCAN
                     KFAPLATPAPPVGRRNKPPYDGCEPMKSDGKLLAETWTRLGEYHFDRGELDAAIFAYQ
                     QAIPFRDSPYFDKALYKLAWSFYRADRFTDAVKHFDELVVLADKKQLGEPGKKEGFAL
                     RSESVQYLALSFAERDWDGDGKDDPVAGLSRAEVFYRGREREPHVREVFQRMGDVLFD
                     RTEYGRAAEVYRMAIATWPLAPENPRLQDRVVVAMERLRNFEQALREREQLARTYVEG
                     SEWAKQNRDNDAALAEGLQLADLALMNVVLRRHESAQKLREEAQAKNDPKLRAQAVED
                     YRQAASSYETYLKQHPNSKDRYEYSYYLAESLFFGEKYPEAAEAYEKVREADPKGKYV
                     EDSAYGAIKSREAVVSALYKQGGGIEPPLPQVGKVTPPVSPLNLPDEVAKLQYAYDRF
                     GVLLPDSPKIALFSYKAAEIDFRYLRFSQMRLRMADIVAKFCKDERAVDAGNALLVSY
                     NIEGDLEKLEEWTARLKDKGCGGSSQVAQTQLGSIKKLSQQVRFKKAEQLLAQKRHAE
                     AASLFIELVGQDPRGPDSDKALFNAAVAQESDKRYGAATETYERIVREYPQSKLVDEA
                     LFRAAVNHQRFFDFDKAVTAYQQLGTDPRFKGSTHRHDALYNAALIAENDQRYALAAD
                     LWKQYAQSDKTSAEEAATAYFRAALSIEKLGPASRAEQELSQFVGRYGQSASSLPQVV
                     EAYVRLSRVQQKQGNPFDATENLKRAAQLGQKLPPGSDAAEFAAEAAFLLAEQKLQEV
                     ERQKIGGSGKELEASISAFNKKVQETVVVYDKVLSYKRANHTLAAYFRMGYVFELYAK
                     AFLAAPCPPEVKRLGAEACDLYKNKIEENVAGIEEKALQRYAVTLEQAAKLGVGNQWT
                     KLARQRANAYRPDAYPLVKDEHVAKQLVLAGPVQAQDGSELAKLAKEARSAMYSGQYE
                     NAIVLAKLVLSKDDHHVPAMLTLATSYYFLGKRELSAAIVGISQTIDANNAEAYVLLG
                     YLALGKDDRIAATAAFKKATELDAGMGLAWHNLSAMYLLAKNYEQALFACERSTVLLP
                     GLSGTQLNFGSALRGMKRYIDADTAYKKVLSAEPQNTDALFNLGILYIDAPSMPGFDS
                     VSQRMIAIRYLESYLDAIGPRGPRDESAEAYIKDARAQLEREQRRQKKKPSPTPPAEG
                     SP"
     gene            42147..42632
                     /locus_tag="PKL17_02625"
     CDS             42147..42632
                     /locus_tag="PKL17_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43649.1"
                     /translation="MRSHGWLCAALCALAFPSVASAQKTETADQSSVPAAKPAAPTDL
                     TAAGPPEKPADAGKASETAQAGDAAQAGDVKKPIDTAPKPGEAGASKPAADAASIKVE
                     RTATGAKLFRITEGLVVEGQMQKPAAFYVLRRAAIHYDYSELGKSFLPKIGAAVKAPP
                     F"
     gene            42643..44064
                     /locus_tag="PKL17_02630"
     CDS             42643..44064
                     /locus_tag="PKL17_02630"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK43650.1"
                     /translation="MTALHDGSAEHGERGSGRAADRALRLGLFLAGKPLEERLFRQPR
                     DISLGKKNDCTVIVPPTEGLSDSTTLFVAGEKGWVLQFGEEFKGRVALSDKPVSLQQL
                     IDDGDAIAHKQLWRVPLPGHAKGELRFGDLKILFQLVPPPPVAPRPQLPSSLRPQLLS
                     TLDKRLAQIAIASFVAHFSFVVYLRAMERPKPAAIEEIPERFVKMLVPKKVEPPKPPT
                     PAKTEAKKADDKKADDKKKTEVAANDKPATQPKKEEDPAAAAARAAEQRAKMEQQLSS
                     MGVLKMLTAKGPDGALADALKDGSGDGDPDKVFREVGGVGPVTGQTGLGTVRGGDGTG
                     TSQSIGSLTTTGPRDGVSTGEKTEKKVRAVVQESSPQDLDPGDLDPGQVAAKIRQYRG
                     AMVACYEAALKRNATLAGKITLRFSVSSAGKVSHVEIEVDTMHDDDFAKCIVERAQSW
                     RFPAPKGGGEVQFAYPFIFQSSK"
     gene            complement(44121..44951)
                     /locus_tag="PKL17_02635"
     CDS             complement(44121..44951)
                     /locus_tag="PKL17_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43651.1"
                     /translation="MTQTRSLSGKTLFITGASRGIGLAIALRAARDGANIVIAAKTAE
                     PNPKLPGTIYTAAEEIEKAGGKALPCVVDIRNEDQIIAAVNQAVEKFGGIDILINNAS
                     AISLTGTLQTPMRRYDLMHQINTRGTFACSQACLPHLLKAQNPHILNLSPPLNMEARW
                     FAPHVAYTMAKFGMSMCVLGMAEEFRDQGVAVNALWPRTTIATSAINNLLGGETVMRG
                     SRKPEIMGDAAHYILTRPSRSCTGNFFIDDELLASEGITNLDHYANIPGEPLFPDFFV
                     "
     gene            45030..45521
                     /gene="mobB"
                     /locus_tag="PKL17_02640"
                     /pseudo
     CDS             45030..45521
                     /gene="mobB"
                     /locus_tag="PKL17_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
     gene            46100..47554
                     /locus_tag="PKL17_02645"
     CDS             46100..47554
                     /locus_tag="PKL17_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNK43652.1"
                     /translation="MSFSRLVRFPKAAALTCALFAACVEQSDDAPSTESLQAAKEHIL
                     SAAPTPRFASGAVLENAKGGRIVYLGADVDVNEVAPGQSFTVTHYFKVEKALGDGWKL
                     FVHIATPDKRLHHNADHVPIGGKYPVSMWKDGEIIRDVQKIPLPQNFTNDKMAMFVGF
                     YKKNERLAVKSGRQDGKNRVIALELPVKGGTQLSSQHKLQVRHLGPGQSILVDGKLDE
                     PAWKNAVSSGLFVNTMNGSPVEQPAQARLLWDEQNLYIGFEFADRDVWGTLEKHDDKL
                     WTQEAAEVFLDPDGDGKTYVELQVSPRNVTFDSWLPGYRQNDNAWDSGMQTAVKVDGT
                     LNQRDDKDTSWTVEMRIPMSAVKGRLETVKGVPPQVGTEWRANFFRMDQENGKPQTAS
                     AWSPPLVGDFHALDKFGSLVFADAGGNVTPPPAPVPQAPAQSAPPAVAPSTDSPGGPH
                     KLNPLLEHAMKGDDSPAPAPAPAAKPAASSPKNK"
     gene            47598..48245
                     /locus_tag="PKL17_02650"
     CDS             47598..48245
                     /locus_tag="PKL17_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNK43653.1"
                     /translation="MSHHTHPKFELIYFPIHGRAEVLRLTFALAQAEFTDVPVTDWMT
                     LKPQMPLGQVPVLKETHGDESWMIPQSGAIIRHLGRVLHLYGQNEREHTICDYVLESA
                     MDWRAKFIPVAYGKMYGTPQDVQDKYWNELCDHNLRLLDKLLASSAKTGEWFAGAKPT
                     IADVAVWDTMDSNLAKRADVLDKYPALRSFYDRFAALPGIAAHLGSRRPSEHRQA"
     assembly_gap    48314..48413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         9414 a        13895 c        15392 g         9722 t
ORIGIN      
        1 tgcatcacgc ccacgaaaaa ggcgtcatcc accgggatct gaagccgtcc aacatcatgg
       61 tggaacggcg gggggatgga tcgtatgtgc cggtggtgat ggactttggt ctggcgcggg
      121 aagcgggtga aggccgcggt ctgacagagt cgggcgccgt gatgggtacg cccgcgttta
      181 tgtctcccga gcaggcgcgt ggggaagctc gccacctgga tcgtcgcagt gatgtgtaca
      241 gcctcggcgc gacgctgttt gaagtgcttt cgggacgggc accgtttgac gacgacaccg
      301 tcatcaacct gatcatgagc gtgatgacgc gcgaggcgcc gcttctgcgc accgtcgctc
      361 cggctttgcc agaggcgctg gagatcatct gtgcgcgctg tcttaccaaa gacaaagaac
      421 agcgctatcc gacggcgcag gcgctggctg acgacctgac ccgctttctc cacagtgaaa
      481 agatcgtcgc caaaaaagtc ggtctgctgt atcggctgaa gtggcgggcg cagcagaaca
      541 aaccggcggc ggcgctggtg ctgtcgttgt ttgcgtcggt ggtgggtttt gccggatatg
      601 gactgcggca aagctatgtc gcgcgcaaac agtccgagct acagcggcac atcgcgcaag
      661 acagcaaaga cattgaatgg atgattcgca cagcgtattc gctgccactg cacaacgtcg
      721 aacgcgaaca agacaaagta cgcaagcgaa tggatcaggt cgaatacaag ctgcgtacct
      781 atggcgattt ggcggcgggg ctcggacact atgcgcttgg gcgtggacat ctgtctttgc
      841 atcagcacaa aaaagcgcgg cagcacctag agcaagcgcg cgacctgggg gaaaaaggac
      901 cggagctgca cgcggcgctc gggcgggtct tgggcgagct gtatcacgaa gcgctgctcg
      961 aagcgcggcg atccggtgaa aaaacctggg tggaaaagcg gcagaaggaa ctccaggccg
     1021 agctgctcga tccggctacg ctgtatttgc aaaaagcgct ttctcgacgg catgagctgt
     1081 cgctcgattc ccccgaatac atcgacggac tgctcgattt ttattccgga cgcgaacagg
     1141 aagccgatta caaagcccag cttgccctgg tccaagcgcc ctggctttcc gaagccgcca
     1201 agctgcgtgg tgaaatcgcg caagcccaag cgttgcgcaa agtgagcgat ggacaacacg
     1261 accaggcgcg caccctgcta agccaagcca tcgaacattt tcgccaagct tccgatatca
     1321 gtcgttccga tggacttata tcggctgcac tttcgcgtgc ctatttggat cttgcggatc
     1381 tggatcgcct acaaggcaag ccacaagagc aggattttga aaaagcgcaa gccgctgccg
     1441 aaaaaaccat caccgcccta cccagcgatg ccagcgggta tctggaaaaa accagaatac
     1501 tatattttaa agcaaagtcg attaagatta aagggaataa aatcgaaatt ctgaatgaag
     1561 caattcagtt ggcagatatt gcgattggtc gagataagaa taattttata gcttgggatg
     1621 ttcttggtaa tgcactgaca acacgcggta tttttgaaca acgcatcgga aaagatccaa
     1681 taacgtatta tcaaaaagca gaaaaaattc tgcgtcactc aatcgagctt gctccgaatt
     1741 ttccttgggg acataatgat ttagggattt gtttcacaca atgggggaat tacatactgg
     1801 atgttggtaa agatccacgc gagcagtatc gttctgcaat agatagcatg aaacacgcga
     1861 tttcactaga ttctcgatat gtttttgctt atgggtcatt ggtgggggca ctgagtcaat
     1921 tttctgatta ctcgttgaat catggtcaaa gctttgacgc gctaatgaga tcagcagagg
     1981 agctcacaca gcagtgcgga acaggctgct ccagctattt gacatttcag cgcaacatgg
     2041 ctgtgctgtg gattaaccat gggctgcatc tgcttcagcg tggggatgat ccgacggtgg
     2101 cggtatcgac agcccgtggt tttctggcga ggcagcggca gcttgataag aacatgtatt
     2161 cagaatgtct attttctcga tatgcggatt ttgctgaggc tgggtgggcg ctaaaacaag
     2221 accgtgatcc aactggtctg gcgaatcggg cactggctgc gacagagctt tgcaagcgat
     2281 tcgatgacaa ggcgcaagag ccttttatga agtctgctcg gtttcatttg ataaaagcac
     2341 agtggcagct aaaaaagcgt caaacggcat cggaagcgat caatcaagca ttggccgatg
     2401 cgtcgcaggc tgtgtcgctg gacgctgaat ctgttgattc ctgggtggaa aaagcgcggg
     2461 cgagctatta tttggcaacc acactgcaag gttctgcaca agccctgcgg cttggcgagg
     2521 ccttggctgc ggtggaacgc ggcttgaaag tgaatggcag tgcgggtccg ctgtgggcgc
     2581 ttcacgcagt gattcggcgg gcgcaggcgc gggcagaaaa aaccccagat gtggcgatgc
     2641 gctggcggaa agaatcggac gaaagctgga acaaagcgct ggcgataaat ccgctgctca
     2701 aaaacgatta tggggatctg ctgggcgagt aggtccgccg cgagtgcgcg ggggaggctg
     2761 ggcaagccgg ctttggcgcc ggctcttggc gactacggaa tcgaatccat caggttgtag
     2821 ttgcggcggg cgtcgaccgt ggccgcatcg caatcgggca gcgcgcaggc catggtgatt
     2881 tctttgtggc ggcggttgtc tggaatcacg cagaaagcgc cgtctggtcc ccagatcgcc
     2941 tcgaggttgg gcatcggatc gcggtcgcgc gccggatcga tcggtgcgtt gatcaggtac
     3001 ttggtgccat cgagcgtgtg cgacttgccg atgccgcagt agtcggcggt cttcatgcga
     3061 atgaacgcct gctggaaatc ggccatattc gcttccatgc ctgcatcgag cttgcccgtc
     3121 gaccacggcg tgtaaccacg cgccagacct tcggcaatcg cgccttcttc acaggccacc
     3181 gtgatcgcga gcggatcgac cgtgcgtccg taggtgtcgg ggtcccacag atatccctgc
     3241 tcgaacacca cgtattcatc agcgcccccg gtctttttgc acagactggt tggcacttgg
     3301 cctttggcgg cggtgttgac gtactggccg acgacatcgg cgccccacgc cttgacgtat
     3361 tcgaccggat tggccccgga cgtaaacgtc cactggaaca tggtgacgcc gcggcctgtc
     3421 gggttcggga tcgacaacag catcgccagc tttttgtcga acatcgagtt cacatccagc
     3481 gtgtcgcaga gggtgccggt tgcgtcgcag aatgtgatca gaagccgagt ctgcctggct
     3541 tccatgaact tgaccttgag cagattgcca tcgaccaaca tgccttcgac ctggccatcg
     3601 ctcttgacgt ggtgatcggg ggccaacgga tctgggctca tgctccagcc gcgcaccttc
     3661 cacttgcctg cctcgggata aaagccggtc accatgtact caaagcccga ccaatcggga
     3721 tatccaatcg cgaacgagta gtccggtttt tcggggttga agattttgct gccgttcgga
     3781 tcttggcaag caccggtctc cggatcgcgg gcctgacagg ccttgagctt gacttgcacg
     3841 ctgtcctgtt cggtcgcctg gttggcgttc tggcagccgg tggcagcgag gagcgagagc
     3901 ccacacaaca tcgaaaaaat cgagcgcttc ataacaaacc ccctgaaatg ttccgttgga
     3961 ttgaaccgag aacctcgttg gtggaagtga ttccgaatcc cttgggatga aactactttt
     4021 tctccaccgc cgagcatcta cacccatgaa caggcaaagc gtatgccagt ggtccgtccg
     4081 ccggatctgt accaattggg acaccgtatt ctgtttcatg gaagaggaca ccttgggttg
     4141 cggcagccgg attcccaagc gattcccaaa cgattcccaa gcgattcctt ttggttggga
     4201 aggacagcct cgatggcttc cgattgtctt tttcgaacga aaacagagac gaaggagtcg
     4261 gatgtcctac ctgggccgca aaggaatggc acaactgtct ggcacagcct tgtgccatca
     4321 catgtgccag cgttccctct cgtataatgg ccctatgtct gaccatgcct cgactttgaa
     4381 ggaggccgta acaccccgga gtgatgagcc actgggcgtc gaacggaatc ctggggtggc
     4441 gctgctgttt cacgccggta cgccgtgtgt gctggccatt cctcttcact gcggagcggt
     4501 ggagcttgga cgtggtacgc atggcatgga accagaccag cggatgtcgc ggcggcatgc
     4561 gcggatttcc tatgacggca ggcgtttttt tgtgaccgat cttgggagtc acaacggaac
     4621 ctctgcggat ggacatgcac tgtcggcaag cgcacccgtg gaagtgcagc gggtggtgcg
     4681 gatgggagac tcgctgtttg ggctgtttca tgacatcgcg ccgcttgtgc agcgcggcgt
     4741 ggtggtaagc caagggaagg tgatggggcc gctgctccaa gatgtgtttg cgctggccaa
     4801 gcagtcggca cagtacggcg ataccctgca catcactggc gaaagtggcg tgggcaaaga
     4861 gatggtcgcg cagggattcc atcagtttgg tccgcgcgcc ggcggtccat ttgtcgccgt
     4921 caactgcgcg gcgattccgc aaggtgtggc cgaacgtctg ctattcggga cgcgacgtgg
     4981 cgcatattcc ggggccgacg ccaatgcgga cggatacatt caagccgcgt cgggcggcac
     5041 gctgtttctg gatgaagtcg gggatctgga tctgcaagtc caggccaagc tgctgcgcgt
     5101 cattgaaacc aaggagctgt tgccgctcgg ggcaaaccgt ccgcatcgcg tcgagctgca
     5161 catctgctcg gcgaccaaca aagacctgaa gtacctggcg gcgcagggac ggtttcgcga
     5221 ggatctgtac taccggattg ccaggccgtg cgtgaacatt ccgcctctgc gcaagcgaat
     5281 cgaagaaatc ccgtggctga ttgattcggc agtgaaaaaa accacgacca ctgcggcgat
     5341 ccacgcttcg ctcgtcgagc tgtgtctatt gcgcccgtgg cctggcaaca ttcgcgagct
     5401 tctcgtcgag gtgcgcagcg ccgtccaaaa tgccgcgatc aagtcatcga cgcgcatcga
     5461 aggcaagcat cttcccgacg gtgccggcac ggttttgcgg tcgcttgggg cggtggcgga
     5521 ttttgtccca gacgactaca ctccgccggc agtgcgggcg tccgatccag gggagctggc
     5581 ccgcatcgaa caagcgctgc gcagccatca aggcaacgtc gctgggaccg cgcgtgcgct
     5641 gggcctgcac cgcacccagt tgcgtcgact gattgaacgg catggtatcg atccgcagcg
     5701 ctttgctccc gatgggacgg cgcacaagct ggatctcgac aagtagccgt cagggcgtaa
     5761 gcaccacttt gagctgcgac ttggtcatcg cgtccgcaaa cgccgccagt ccgtcggaaa
     5821 gcgcgtagcg ggccgagatc atcggatcga cgatgatccg tttctgcgca agcagccgca
     5881 aagccgccgc aaacgggcca cagcgcgaac cgacgacgga tatttcatca atgacgatct
     5941 gggtcagcgc cgcggcaaac tgcgctgcgc catcttgtcc gttcgcattg tctttgacca
     6001 ccgacggagg tgcataggtg cttttcaaca ccaaggtgcc gcgcggacgc aggattttga
     6061 tcgcctgtga aagtcccgac ggcgagccgg tacaatcgac gaccacgtca aaccgggctg
     6121 cggcatcgtc acccagctgc gaaagcagcc gggtcgcgag cggatgccca gaaagcagcg
     6181 ccagcttgtg tgggtgtttg ccaatcgcgg tgactttcgc ggcgctcgcg tgggccagca
     6241 cctgcgcgca caaaagccca agttttccgt cgcccagcac cgcgacatgg tcggtcggcg
     6301 ccatgtggac ttggttggga atttgcagcg ccgcggcgag cggttcgaca aagatcgctt
     6361 ggtcatcgct gacttcgtcg ggcacccgat gcagattttg gaccggcacc gtcaggtact
     6421 gcgcaaacgc gccattgcga cgaaaaatcc cgagtgtggt gcgacaagga tcgtgcgcgc
     6481 gcaaggccgc agtgggcgga cttggcgggg gtgaacagtt gatttcagcg acgacacgac
     6541 agcctgtttc aagcggtctt ccggcttcat cgacatgccc cggcggaact tcgagcaggg
     6601 tgccgacaaa ctcatgaccg ggaatgccgg aaaaacccat gtaaccgcgc agaagctcga
     6661 cgtcggtgtt gcagattcct gcttggcgga ctgcgacgag cgcttcgcct tccgtcggaa
     6721 tcggatcggg caccgaatcg caatacgaca gcgtttggga aagcacgaga gcacgcatgc
     6781 ccgcatgcta cggcgaactg cgttttcgcg cgccttgcaa acaggcaaaa ccgcgtacca
     6841 tggcgcagat gacacacgca cgtagctacg gtcttgagtc gctcgatttg ctgtcgacgg
     6901 tacttgcgaa agatgattcg ggaacgccgg tctgcgagct gcggctggat acgcagtgca
     6961 tgtcgctgcc gacggacttt gtgcatcgtt atggcgcggc gctttcgcgg gcccacgctg
     7021 caatgctggc gctggaatcg ggggcgattg caaaccccga tgaaaaacgg atggtcggtc
     7081 actactggct gcgcgatcca tcgcgggcgc cgaatccgga catcgccaaa gagatcgaag
     7141 actgccgaaa acgaattgtg tcgtttgcag atgccgtcca tcgcggcgaa gtggtttctt
     7201 cgacgggaca gcgctttcgt cagctcctgc tcattggaat tggcggatcg gcgctcgggc
     7261 cacagctcgt tgcggcggcg cttggaggga cggcggacaa gctgcgtccg tattttttcg
     7321 acaacaccga tccggatgga tttgatcgcg tacttgcgca gctggcgacg ggcgatggtg
     7381 gacttagtac aacactggtg ctggtcgtat cgaagtcggg cggcaccaaa gaaacccgca
     7441 atggaatgct ggttgcggaa gcggcgtttg cgcatgccgg tctgccgttt gctcgtcaca
     7501 cggttgcgat tacccaagat ggcagcgagc tcgatcagac cgcgcagcgg gccggatttt
     7561 tggccacgtt tccgatgtgg gatttcgttg ggggacgcac gtcggtgctg tcagcggtgg
     7621 gtcttttgcc ggcggcgctg caagggatcg atatcgacgg gctcttgcgc ggcgcacgca
     7681 tgatggacca agcgacgcgg cttgccttcc ccgaacaaaa tccggcgatg aagatggcgg
     7741 tctgctggtt cgatgcgacg ggcgggcagg gcaagcgcga cttggtggtg ttgccgtaca
     7801 aagaccggct cgagcttttg tcgcgctact tgcagcagct catcatggaa tcgcttggca
     7861 aagaacacga tcgcgacggg cggcaggtgc atcagggcat cgcggtctat ggcaacaaag
     7921 gcgcgaccga tcagcatgcc tatgtgcagc agctccgcga cggtgtggac aactttttcg
     7981 tgacgtttgt cgaagtgctg cgcgatcgca gccagccgtc gccgttggtc cctgattcgc
     8041 tgtttgtcga acagcaggtc agcgcaggtg actatctgct cggttttttg ctgggaacgc
     8101 agctggcgct cttcgaaaaa ggtcgacgca gcctgacgct gacggtgacg gaactgtcgg
     8161 cgctcacgct cggggcgctg atcgcacttt tcgagcgcac cgtcggatat tacgccgagc
     8221 tggtccatgt gaacgcctat catcagccgg gtgtcgaggc gggcaagcgg gctgcggcga
     8281 gcgtgctgtc gctgcaaaaa cggctgctgg cggttgtcga cgacacgccg cgcactgcga
     8341 ccgagcttgc cagccgagcc gaagcgttcg atgacgacat tgtggctttt aagatcctcg
     8401 aacatctggc ggaaaacggt cgtctgcgtc gccaccgcga tgccgacacc gcgattaccg
     8461 cagcgcgcta cagtcgctcg ctacgccttg gatgagtgat ttccgatgac acaagatgcg
     8521 ccgatttttt cttccgtcgc cgatgtgagt gagcggctca ggtcgcaggg ctacatcgcg
     8581 agtccgcaga ttgcgacgag tgtgctgctc gccgagcgac tgcacaaacc gcttttgatc
     8641 gagggaccgg cgggctgcgg caagaccgag ctggcgaaag tgctttcgca gacgctgggc
     8701 accgatctgt ttcggctcca gtgctacgaa ggtctcgacg aggccaaggc gctgtacgag
     8761 tgggcgtatc ccaagcagct tctgtacacg cagattctgc gcgacaaaat tggcgacctg
     8821 ctgtccggtg tgggctcgct cgccgacgct gtggccaaga ttgccagcca cgaggatgcg
     8881 tttttttccg aacgcttttt gctcgagcgg cccctgctca aagcgctgcg ggcaaagcgg
     8941 cgtgtggtgc tgctgatcga cgaagtggat cgcgccgagg ccgagtttga ggcgtttctc
     9001 ctcgaagtgc tgtcggattt tcaggtctcg attcccgagc tgggaacgct gtcggcccgg
     9061 cacatgccgc ttgtgatcct gacgtcgaac aacacgcgcg aaatgaccga tgcgctccgg
     9121 cgtcgttgcc tgtatctgca catcggtttt cccgacgctg cgcgtgagct gcaaatcgtc
     9181 cacagccggc tgccgcaggc gcccagtcag ctcgttaccg acgtggtgcg ggtggtgcag
     9241 gcgctgcgca cgctcgatct gaaaaaaaat cccagcattg ccgaaacgat cgagtgggtg
     9301 cgggcacttt tggtgctggg ccagacccat ctggaaccgc agcttgccga ggagacactg
     9361 aacttggtct tgaaatacca gggcgacctc gacaccgcgc gtgagcaaaa aaagacgctg
     9421 ttccccgacg ccaaacgcta atcgtcccat acaattgcga acatgcgagc tgtcagtccc
     9481 tcgccgcgct acgacgcggt ggcagacctg tgggtgattg cggcgtacta caatccgcac
     9541 ggctacagga cgcgcgccca caactacgcg ctgtttgcag acagtctgcg gcgcagccac
     9601 attccgctgc tcaccgtcga gtgcgcgttt ggcacagagc cgtttgtgct gcccgatggg
     9661 ccggatgtgc tcaagctgcg ctgcgacgcg gtgctgtggc aaaaagaacg gctgctcaat
     9721 gtcgccgtcg agaggctgcc gccaacctgt accaaagtgg cgtggctcga tgccgacatt
     9781 ctgtttgagc gcgccgactg ggcgacgcag acctcgcttt tgctcgacga aaagcagatt
     9841 gtgcaggtgt tttcgacggt ggttcggctg ccgcgcggcg ccacgtcgct tcctgcgcta
     9901 cccgacgggg aatcgcccga gcttatcgag agctttgctt ccgtgtatgg ccgcgatcca
     9961 tcgctgcact tgggcggcca ctaccacgag cacggacata caggttttgg ctgggcggcg
    10021 cggcgcgacc tgcttttgcg cacaggactt tacgataccg cgctatcggg gagcggcgat
    10081 cacctgattg cgcatgccgc ggtcggagat cacgagtcga agtgcatcga caaagtgctg
    10141 ggcaaaagta ccccgcatcg cgaacacttc cgtcgctact gcgatcggtt ttttgcccgg
    10201 agccgcggtc agcttgcggc ggtgccaggg acgattttgc atctgtggca tggcgatcac
    10261 gaatatcgac gttattccga ccgcaatatc gaacttgtga agcttggatt tgatccagcg
    10321 cgtgatcttt gtttggcaaa tagcggtgca tgggaatggg gtagcaaaca tcccgaactg
    10381 cacgcctggg ccaaagaata tttcgcccgc agaaaagaag acggcgacga gtagcccgcg
    10441 cgctggacgg cagccgtttg ggtaaaaacg gcaggatttg ctaggggaac ggagctggta
    10501 aaggtagtgt gccgatatgc cggtttcacc agatgccttc gctagccagg cacttgctgc
    10561 acgcgccaaa attgtggcgg cgctcgacga aattcacggc attttggccg attgtgacga
    10621 agacgaagtc gcgacctgga tggcacaagc gcaagaactt cggcgggcgc tttcgatcat
    10681 agacgagcag cttcagctgt ttcggtccga cccggcgcaa aaagatttgc gtcactgacc
    10741 gcttgcccgc agcgttcggc tacaaggcgt ggtatgtaag ggcctatgct gctcctccgc
    10801 ttgctgcaaa agccggatcg tgcgcctcgt tttggtcgtg ttctcgacga tgaacctgaa
    10861 gtcgtcgagc tgcttgatgg cgatccgctg ctcggcggca agccaactgg ggaaaccctg
    10921 gcgtttgcgc cggctgaatg gcaagccgat cccgacggaa gtgagatcga gctgctcgtt
    10981 ccggtggtgc cacagaaaat catcggcgtc ggcagcaact atcgcaacca cgccaaagag
    11041 atgggcaagc cgatcccgag cgagccgctg cttttcctga agccgccgtc ggcgcttttg
    11101 tcaccgggac gggcgattgt gcggccccag ggctttgagc gctgcgacta cgaaggcgag
    11161 ctggcggtgg tgattgggcg gcgctgtcat cgtcagccgg tgtcgacggc gatgaactat
    11221 gtgctcggct acacgctgtg caacgacgtg acggtgcggg atctgcaaaa aaaagatggg
    11281 cagtttaccc gcgccaaagg ctttgacagc ttctgtccgc ttgggccggt gatctcgacg
    11341 gacatcgacc tcgggcggac ggcggtgcgc agccacctaa acggcaagct ggtgcaagac
    11401 ggtcagagcg acgagctgat tttcagcatc gaggagctgg tgtcgtttgt gtcgcaggtg
    11461 atgacgctgg aaccgggcga tgtgatcacg acgggaacgc cagcgggtgt cggcaacttg
    11521 caaccgggtg acgtgatcga gctgtcggcg gatggcatcg gttcgctgcg caatccggtc
    11581 atcgcgggtg cgctgctgcc ttctttgccg gcccgaagct aacgacgcag accccgcagg
    11641 ttttggcggc tattttccgc ccggattttt gtcgtcaccg ctccctggaa tcggcggcaa
    11701 gagcgtgcgg gtgggcgtct tggggggcgt cgccggcggg cggtcttcgg cggtccgtgc
    11761 cgacagtcgc atcgtcggac gaatcgtcag cccgcggtcg ataagcggca ccgtcgcttg
    11821 ggctttcgcc agctcgacga agcgccactg cgatcgcagc ggcacatccg tcggtccggg
    11881 cacgatccgt tcatagctgc cgtcgcggcg caacacccgt gcgcgctggt tgtcgagcaa
    11941 agaaatcgcc aagatttcgt cgacaatccg ctctttcagc ttgatgtctt cgacggggaa
    12001 catgacttcg acgcggcgct ggaagttgcg gttcatgaag tccgcgctcg agatgtacac
    12061 atcgcgcttg ccgtcggatt caaaataaaa aatgcggctg tgttccagaa aacggtcgac
    12121 gatcgaggtg acgcggatgt tttccgaaag ccccggaacc cctggccgca gacagcagac
    12181 gccacgcacc atcaggtcga tctgcacgcc ggcttgcgag gcacgacaca gtgctttcat
    12241 cacgtcgcgt tccgacagcg cattcatttt ggcgatgatg cgggcgggtc tgccggcgcg
    12301 ggcgttttgc gcttctgctt cgatgtgatc gagaatgcgt tcgcgcagac caagcggtgc
    12361 gacgacgaaa cggtgccagc gcggcggctg cgaatagccg gtcagcatgt taaacagcgc
    12421 actggcgtcg atgccgaact cctcgcggca ggtgaaaaac gacagatcgg tgtaaatttt
    12481 ggcggtctgt gagttgtagt ttcccgtcga caaatgaaca taacgacgga tgccacccgc
    12541 ttcgcgtcgg accaccaggg ccactttgca gtgggttttg agaccgatca gaccgtagac
    12601 gacgtggacg ccggcttctt ccagtttgcg cgcccagacg atgttgtttt cttcgtcgaa
    12661 gcgggctttt agctcgacga gcgcagtcac ctgtttgcca agctctgccg cccgcaccag
    12721 cgccgcgacg atgggtgagt ttcccgacgt gcgatacagt gtcgctttca tcgccaggac
    12781 attcgggtct tcggcggctt cctcgataaa atcgacgacg ctctcgaacg actcatacgg
    12841 ctggtgcaag aggatgtcgc cttgggcaat cgcctcgaac atcgtgctgg catcttgcag
    12901 acgggccggc actttcggca caaacggctc gtcgtacaga tcgcgcgcct cgctggtctg
    12961 ccccttggcg tacacctcca tgaggtcggt caggttgagc ggccccgtca ccggatagac
    13021 atcttggctg tccagctcga gcgcgcgttc gagaaaacgc cgcaccgcaa catccattcc
    13081 cgcgtgacag tcgagccgca ccgccggttg acgatcgcgc cgccggactt ccttctgaat
    13141 cgtctgcagc agatccatcg actcttcctc gtcgatggtc atgtcgtagt tgcgcagcac
    13201 gcggaacggc cagcagccca aaacccgcgt ccccggaaac aagtcagcga cgtgcagcga
    13261 gatcacatct tcgagacaga cgaacacccg ccgcgggcct ttggttggca cctcgacgag
    13321 ccgactgaac acgctcggca cttgcatcac ggcaaagctg gcttcctgtc cgcgcagccc
    13381 ttgggcaccg cgctgcagca aaatcgccag gttcagcgtc ttgttgcgca cattcgggaa
    13441 cggatgcccc ggatcgacgg ccagcggcgt cagcgccgga tagacctgcg cattgaaata
    13501 cgtgtacaaa aaacgtcgct gctcaggttc tagttcaccg acggaaagaa gatcgattcc
    13561 cgcctggtga agccgcggct gcaggtcctt gtgccacagc cgatagagat ccgcgaccat
    13621 cttttgggcc cgttgggcga cgcgcgccaa cgcatcctgc ggcagcagac cgtcggcgcc
    13681 ggtttcgatc agaccggaca cgatctgctg tttggtcccc gcgacgcgca ccatgaaaaa
    13741 ctcgtcgagg ttcgagcagg tgatgctcaa aaacttgatg cgctcgagca gcggcaccga
    13801 ctgatccgac gcttcgtcga gcacccgctg gttgaaatca agccacgata gctcacggtt
    13861 caaataaagc cctggatcgt cgagaagtcc actgtcggaa ctgctcgatg gcgacccggt
    13921 agagggcagg ggcggatgac tgggtgtcga tgggaccgtc atgggcacgc ttgagctgaa
    13981 gagttcccat gttagcacgg cgcgcgcaga tcgccggttt tgctcgcgtt ggcgacggga
    14041 tggaaaagct taatgcactg cgtcaacaaa tcagtagaat ccgccgccat gagccagcaa
    14101 gtcgatccga tcctgccgct tgagctgtcg ctgtcgttcg atgatgtgct gcttttgccc
    14161 ggtcactcgt cggtcttgcc aaccgaatgt gatgtgtcga cgaagcttgc gcggctgccg
    14221 ctgcggattc cgatgatgtc gtcggcgatg gacaccgtaa ccgaagaaca gaccgcgatc
    14281 gcgatggcgc tgatgggtgg catcggcgtg ctccaccgca actgcactcc cgacgagcag
    14341 gccaaaatgg tgcggcgggt gaaaaagttt cagcatgcgg tgattgccag tccgatcacg
    14401 gttcgtccgt cgatgacgat tggcgaagtg catcgcttga ttgcggaaac ggggatcacg
    14461 ggctttccgg tcgtcgatga tgcgggcgtt ctcgtcggca tgtgtaccgg gcgtgacatt
    14521 cgttatgtca ccactccgtc gatgcgggtg tgcgatgtca tgagcacgcc ggcacgatcg
    14581 cttatgctgg gtgcgacccg cagcgaagcc gaagattttt tccgtcgcta caagctggaa
    14641 aaactgccgc ttgtcgatgc gcaggggcgg ctcggtgggc ttatcacgtc gcgcgactgg
    14701 cgcgaagcca agagccaccc cgacgcagtg cgcgatgaac agggcagctt gatggttgca
    14761 gcggcgatcg gcgtcgggga agaagcgctg tcacgagccg aagcgcaggt cgaagccggc
    14821 gtcgacatgc tggtgatcga ttcggcacat ggacacagcg acggcgtgct ttcgacggtg
    14881 cgggcgatca agcagcgctt tccgtcgctg cttctgtctg cgggcaacat tgcgacagcg
    14941 gaaggtgcga ttgcgctggc cgaagcgggc gctgacatca tcaaagtcgg catgggcccc
    15001 ggttcgatct gtaccacccg cgtcgtcagc ggcgtcggag ttgcccagtt ttccgccgtg
    15061 atggaagtca cccaggcgcg gagccgtcgt tttcccgatg tggcgatcgt tgccgacggt
    15121 ggcattcgct attctggcga tgtgaccaaa gcgctcgcgg cgggcgcggg tgcggtgatg
    15181 ataggcgggt tgtttgccgg caccgacgaa gcccccggtg agcttgtgct gtaccaaggt
    15241 cgttcctaca agacctatcg cggcatgggg tcgctcgcgg cgatgaaaaa aggcagcaaa
    15301 gaccggtacg gtcagggcgg tgtcgccgaa agttcgaagc tggtgccaga aggcgtcgaa
    15361 gcgcgggtgc cgtatcgcgg tccgctcggc agcgtcgttg cgcagctgct cgggggtctg
    15421 cgatcgggca tgggttacgt cggtgcaaaa gacctgggcg agttacggag gcgggcccgt
    15481 ttccgtcgga taaccgcctt cggcttgcgc gaaagccacg tccacgacgt caccatcact
    15541 gcggaagcgc ccaactactc gggcacgttt gaagactaag tgagcgtccc atgctctgtg
    15601 tcctcgacta cggctctcaa tacacgcaac ttatcgcccg acggctgcgc agccagggct
    15661 ttttcaccca ggtgttgccg gggaatctgt cgccgcaggc agtcgcggcc tacgctcccg
    15721 acggcatcat cttgtccgga tcgccacgca gcgtcggcac cggttttgat cccgatccgg
    15781 ggctgctctc gctcggggtg ccggtgctgg ggctctgctt tggctatcag tttttggcac
    15841 agcatctcgg cggtcgagtc gccgctgcaa cccatcggga atatggcgct gcgatggtca
    15901 gcaagacacc tgcgggtcgt cgcgatccac taacggcgaa gctgtcgcag acgacgccag
    15961 tgtggatgag ccatggcgat tcggtcgtcg atctgccccc atctgccgag ctgattttgg
    16021 aatcggcggg caagcctgcg ggctttgtcg taccgtcgcg aaagctgtgg ggcctgcagt
    16081 ttcatcccga ggtctatcac acccctgaag gcaagctgct cctcgaggcg tttgcgcgcg
    16141 acatttgtcg tctatcgccc gactggaact tgcgggccga gctcggtgag atcaaagcca
    16201 agctggcaaa agagctgcac ggcgtcgatg aagtcgtgtg cgcagtgtcg ggtggcgtcg
    16261 attcgacggt gctcgcggtg cttttgtcgc aggtctgcaa agtgcgggcg ctttttgtcg
    16321 accacggctt tctgcgtgcc tacgacatcc gcaatctccg acgagtgttt cagcattttc
    16381 ccaacatttc gctcgaagtc gtaaacgcgc aagaacgatt ctgggcagcg ctcgacggtg
    16441 tgagcgatcc agagacaaag cggctcgaaa tcgggcggct gttcgtgcgg acatttgccg
    16501 agcacgtcgg ggaaaagcac tttacccatc tggcacaagg cacgatttat tccgacgtga
    16561 tcgagtccgc agccgtcgca cacggtgggg ctgcgcacat caagagccac cacaacgtcg
    16621 gcggttttcc tcaggatgcg cgggtgcagc tcgtcgagcc gcttcgcccg tttttcaaag
    16681 acgaagtccg cgagcttggg gttctgctcg gcatcgacag cgaaattctg caccagcatc
    16741 cgtttccggg gccgggcctg gcgattcgct gcatcggcga gctgcggcgc gagcggatcg
    16801 aaattttgcg gcgggccgac gagatttttt acgaagaact ggtccgtcgc gacatgtatc
    16861 tgcggacttg gcaggcgttt gttgcacttt tgccggtgcg caccgtcggt gtgatggggg
    16921 atcagcgcag ctacgaacac gcgctggcga ttcgggcagt gtcgtcggtg gatgccatga
    16981 ccgccgaagt gaccgagttt tcgctggccg agcttttgcc catcgcgtcg cggctgataa
    17041 acgaagtgcg cggcatcaac cgcgttgtct acgacctgac cagcaagccg ccggggacga
    17101 ttgagtggga gtgaaaaatt tgcccacgtc agagccaggt ggtaacgagc cgcatgccgt
    17161 cgcgttcgtc ccttgacatg tttgcgcccc agctggcgct gcagtccatt tgtgagacaa
    17221 ccccgtcgct gcctggcccc gagctgtcac ttcgtcagca agagcaaaag ctccgacggc
    17281 agcttcgatc aagcctgcgt ctgggcgatc tggtgcagca ggcggcatgc tatctgagcc
    17341 tcggtgacat cgagctttcc cgcggtgaca gcgaacgcgc gggtgagctc tatcgacggg
    17401 cgctcaagct atcccaaacc gcgcatgaga gccggcaagc gaaagcccgc agcagtgttt
    17461 cgcacaagta gacccttgct ctgtgcgtcg gtctgcgcta cccaaggtct ttgccaatgt
    17521 ccaagccgcc aaccaagtgg tcctttcctc gtcgaacctc gtatggggtg ctcgagcgct
    17581 ttttgggcca agagatcaat gtcttcggcc cgcgtcagag cgacgaacac gacaactgtc
    17641 taggcggagg ctttctcgtc agcattgagg acggcttttt gctgctcgct gagcgaccga
    17701 gcgaagcccc cgatctggcc gttgcactgt cagaagtcgt ggtgattgct gtcgtcaaag
    17761 atcggcccga actcacggcg gtcgatggtg gcaaaatcca tcgcttccgt cgtcccgatg
    17821 cgcctggtcc tagcgacgac gatctcgacg acaactagcg tctttgggtc cgcgtacaag
    17881 ttgggtttgc gcgtgcggaa tgtcggcata tagtcggtgc atgacgcaca cagcttggtt
    17941 ccgtcgtggc ggtttccgca gtgctgttct atttggtgca cttgccgtcg gacttcttta
    18001 cgtccccaag gccgctgccg atccaaaacc gtctttgcgc agtcgggtgt ttggctatat
    18061 cccgtactgg acggcgagca aaaagacctg ggatttgcgc ggtctgaccg atgttgcgtt
    18121 gttttccgtc ggcgcgagtg acaccggcac cctgaccagc acgacgtttt ggcgtggcaa
    18181 agacgcccgc gcgatcatcg acgacgcaca caagcaaggc atcaaggtcg agctggccgt
    18241 caccaacttt gatccgacga gcctacacaa cttgctgtcg agcacgagtg ctgtcgacaa
    18301 gcttgtcgcg gcgcttgtcg aagagtcact ggttacccag ccgggcgacg gactgagcat
    18361 tgattttgag gggctaagag ctgccgatcg ggcggcgctt gtctcctttg tcaccaagct
    18421 gcgggcggcg atgaaggcca agcgatcgga ctcgcagctc tcgctcgcga cgccggcagt
    18481 cgactggagc aaagcctacg actaccctgg tctcgccgcg cagagcgaca cgctgtttat
    18541 catgggctac ggctatcact ggagcggtag ctcgacgccg ggcccaggcg ccccgctaag
    18601 ctgcggtgca ccgtggggga gcatttgcct tgcggcgacc gtgaaagact acgtggcggc
    18661 gcttggagac ggcctgcggc acaagattgt gcttgggctg ccgctgtacg gttacgacta
    18721 tcccgcggat tccgacaaaa tcggcgccaa agcgctcggc acgggcacgg cggtgctgta
    18781 ttcggcggcg cgcaaacagg tgacaaaccg tcgctacgag cccgtttcgc agacgccatg
    18841 gtacgtctac accgacgcca aaagcgtgat tcatcaagtc ttttacgaag acgaagagag
    18901 catcggcaaa aaaatcgatt tcatcgttga gcagaagctc ggtggggttg gtttttgggc
    18961 gctcggctac gaagacgaca gcctgtggat ggtggtcaaa gacaagctcg ttgtgaccga
    19021 tccgccgccg ccgctgccgc ccccaccacc tgtcgtcgat ccgccgatga atcccgaaac
    19081 cggcagtgtg gccgaagccc aaggctgcgc actttcgtct cgctcgtcgc acatgccaac
    19141 ctttccggcg ttgctcggcg tcggtagcgc aacaattttt ctgctcggca aacgccgacg
    19201 gcggactgtg cggtacaatc gtccggctcg tgccgtctga actgcgcatt atttttcgta
    19261 ctgccgttgc gccttctggc cgcttgcata aggcttgaga acgtaggtgc cccttgtctg
    19321 agcaatcgac cccgtcctct tctctgtcgg taaacgccat cgccaagctg gtcatcacgc
    19381 tgctgtgtag cttcgttgcg atcgggctgc ggctttctca tttccacttg ccaccgctcg
    19441 aaggtctcgt cgccaacggc acggcggtgt tgtcggcggc gttcatcttg gcgtgggccg
    19501 ccgaagcgct tcaggtcgac attgccaaag gtttggcgat ggcggtgctg gcgtttatcg
    19561 cggtgctgcc ggaatacgcc gtcgacctgt actttgcgtc gatggctgcc aagcatccaa
    19621 gctacgcgca gtatgccgcc gcgaacatga cgggatcgaa ccggctgctc atcggcgtcg
    19681 gctggtcggt ggttgcgttg tgcagcgtct atgcggcgct gcgcaagcgc gatcccaatg
    19741 cccaaaaaga accctcaccg ggtgtctcgc ttggcgacgg ttatggcgtg gacctgatga
    19801 tcctggcggc ggcgacgctg tggtctttga cgatgccgct gtttgaacag attgcgctgt
    19861 ggaacggggc aggcctgctt ctgctgttcg gtttctacgt ctggcgggtg tctggtggcg
    19921 ccgatgccga accagagctg acgggcgtgg cgcaggagct gggatcactg ccgaaagttc
    19981 gtcgacgggt cacggtggcg ctcctcctcg ttgcggcggg ggcgaccctt ttggcagcgg
    20041 cggagccgtt tgctgaatcg ctgattgcga cgggggaaca cttcggcgtg gatcgcttcc
    20101 tgctcgtgca gtggctggcg ccgcttgctt cagaagcacc ggagctgctg gtggcgacga
    20161 tttttgcgct gcatggacac ggtgcggcgg gcatgggcgc gctgttgtcc tcgaaagtga
    20221 atcagtggac gctgctcgtt gggacattgc cgatggcgta ctcgtttgcg ctgggcaagc
    20281 caggggcgct gcatctggac cctcggcagg tcgaagaact ggtgctgacg agctcgcaga
    20341 gcttgcttgg cgttggggtg ctgctaaacg gtcgcttgga ggcgtgggaa gcggtggtgc
    20401 tgctgatcct gttccttgtg cagcttccct tcccgcaagc gagcgtgcgc tacggtctgt
    20461 cgatgctgta tatcgcgctg gcgatcgttt tgttcctgcg ctacgcccgc tatgttccag
    20521 gacatgtgcg gctcttgctg cgcaaggaat cgtctgacga aggctaggca ctgatgctcc
    20581 gggtttcggc gggtctgcgg ctgcttttgt cgccgcttca cacgctctta gagccgacac
    20641 gcgatctgct gttgcgcgcg caagcggcct tgggcgacgg tcagcgtggc gcgatggtgc
    20701 gggcgtttgt cgaactgcat gcgctgcttc tgtcgccgct ggatgcgctg gtcgaaaagc
    20761 gcagcggacg tgggtcggat gactcacctg gttcggcctt gctcggtctt atcgggctgt
    20821 gggacgagct gcccgacatt gacgtcgagc gcgaaaacct gatcgagctc ttggcgcagc
    20881 acgccgcgac accgccggct ttcgatgtgc cgccgccaga cggacttgcc catcacgatg
    20941 gggtttccgc ggcccagcgc gcgtggctag atcaggtgct aagcgtgcgt gcgtgcccga
    21001 cgccgcagtg gtggcgacgg ctctcgcgac gggcgcgggg gccgcttggc gatcgggcgc
    21061 ggctgacggt cgagcggacc caagacgcga cggtcaggcg cattttcgag catctgccac
    21121 ggcctggtac agacgacgac tggaccgcga ccaccgatct tggcgagctt tgcgcaaaag
    21181 atcagctgac gctggctgtg caggtgccgc tgccggggca gatcgcgatg ttgcatttgt
    21241 gcggcgattc gcagacggcg cgactcgatg tcgtgttgcc ggaaacgcca agtgaagcgg
    21301 tggtgcgtcg ccatctggag atcgtcgagc tgcacggaga gctggccacc gagcgacttc
    21361 ccgacggaag cattgccgat caagcgctgg tggtggtctg ggtgccggaa gtgatgccac
    21421 cgtcgtggat cgtcgatatg cagcttcgct ttggcgtacc gcccgaggcc cggatctggc
    21481 gctacgtcta tcgcgtggtc ggctaagcgt tcgcgttctg ccggcacaga ttttcccgtc
    21541 gctggcttgg cctttttacg ccgtgcgctt gcggctaaaa attttcctcg tcgcccgttc
    21601 ggtgttacta cgtgaagtct gcttcccatg ttgcgcagtc gtcacaagcc cgctcgtcta
    21661 agccttggca gtcgatcgcg atcacgtccg cgcagcgctc gctcgcagca gctttttggt
    21721 gcggtcggtt tgcgcagcga gcgtcgatcg ggacagaagc tgtggcagaa gctgcgcgcg
    21781 ccgctccaga tccttggggc gttggcggtg ctgctggtga taaacctgta cgtgctttac
    21841 taccgtcacg ggacgtcgct gccagcgctg ctaaggcaag cagaaacggg acgggcgctg
    21901 agcgcgcggc tgagtggtcc ggtcggaacg ccaccccagc cgacccgtcc tttgcgcaag
    21961 ccgaaagtgc tgccgccgct gcctgactat ccgcgtgtcg tcgaacagcc gctgcatgtc
    22021 agtgattcgc tcggcgacgt gctcaacaaa gtggcggtgc cggcccggca aaagagcgag
    22081 cttgcgacgg cgctgcaagg cgtgctcgat cccggtggct ttgggcccgg tcaatcgctg
    22141 acgttttttt atgaccacga cgaacggctg caagcggtcg attaccgtct gaccgactcg
    22201 ctggcgtatc acctggaacg ggtgcagacc ggaagtgccg atcggtttgt cccggtgaaa
    22261 cagacccagc cgcttgtgcg ctcgacgctg cgtctcgagc tgggttttga agggaaaaac
    22321 gatctgcaag cggcgatgct gcgggccggg gaaacgccgg cgctggcggc tcgagtgtac
    22381 gaagtgctcg cgtgtgagct gaacctgtat agcgacaccc aagccggaga caagctgcgg
    22441 ttgctcgtcg aaaaacagca ccttggcgat cggttttatc ggtacgggcg gctgcttgcg
    22501 gtggaatatc tgccggcgac agcgacaaca ggcgggcgca atcggcggct gcgggcgttt
    22561 ctgcgcagcg aaggcagtcc agtcaacgcg ggggctgctc cgggggcaag cgcgctctat
    22621 tttggcgaca gcggcgatag cctaagccgg agcatctgcc gggcaccgct gctgtggagc
    22681 cgtgccaatc ggacgacgct ggcttcggcg ccgcagcttc agggggaaaa aggtcgctat
    22741 gtgctcgact atccggcgga agcgacggtg ccggtggtgg ccatcgcggc gggcaaggtc
    22801 aagcttgggc cgagtcgacc cggcaaaccg cagacggtgg tggtgcagca cgcgggcggg
    22861 ctcgaaacga gctatgggcc cattggtcgg cttgtgcgcg gacttgtcga cggacagatt
    22921 gtgtaacagc ggcaggtgat tggctatgtc gcgacacctg caggccgtga cgcggcgcat
    22981 cttcggctgc aagcgcggct gtcgggccgt gtcgtcgatc ctggcaagct caagtccacg
    23041 cgcgagccgg gaattcccga tgcggaacgt gcagcctttg tcgcgcagag cagcgactgg
    23101 gccgaaaagc tggcacgcag cgacgaagcc gttatcgcga cccgcggcgg tgagtcgcgg
    23161 taaacagcag cgccagcttc ggttctttgc ggaacacgta ggcgccaccc agcctcgctt
    23221 cggtcatcag ttcgtgtagc accgtcgctg cgcgatcccg caagtcttcc atcacacgtt
    23281 cgtcggcggt cggaactttg gcgggtgccc cgcgggtgct gatcttgcgc gaaagctcgc
    23341 gggctttgtg ggcggtttcc agtcccagac cgatccgcga aagctgcgct ggatagcttt
    23401 cggcgatgag cgcgagatcg agcaagtcgc gcgcgagatc gccgagtccc tcaccttcga
    23461 caatcgcatc gagcgattcg agcactttgc gatctttgcg cagcgtgagc cgtgccgcga
    23521 ggaggatttg attgcgcagc tcgctcgcct ggtgaaaaag cgcaagttct gcggcggcgt
    23581 actgttttcc gcggtgcgca tccaggtcgt cttgggcttc cgaaagtgca tgcgcataca
    23641 gcgacagacc gtcgagcatc ggctgcgaaa aaccaatttt ggccagcttg tcggcgtagc
    23701 gcttggcgac ggaaaacagc gcatgcgctt cgcttaccat gtggggcacc ggctggattg
    23761 gcggcaggac gtgctcgctc ggcacccgca acagcatgtc tttgacccgc ggcaaaacgg
    23821 gacgcttggg ccttgccgtt tcggtgggca aaagtgcgtg ttcgcttggc tctgtggcgg
    23881 gcgcagcgac ggttttggct cttgctggct ttgctttggc gggcgcgttt gtgggcgctt
    23941 tgggcgcagt gggtgcagtc gcggcctcag cgggcttggt cgccacttcg gcggctttag
    24001 ccggagcttt cgctttagca ggagctttcg ctttagcagg agctttcgct ttagcaggag
    24061 cttttgactt gggcttcgcg cttcttttgg cggcgggctt ttgactgctc gccagcgcgc
    24121 ggagtcgctc aagtgcactg cttgtggcgt ggccactggc tggagacccc gactttttca
    24181 cggttgattt ggccatatcg agcatcatgt ccccaatcgg tgcagcgagc gcaagcctgt
    24241 ttgcgcagga gcgcttttgg accgctcgcc tccgtcgaag aagctgggat ggtttgcggc
    24301 tcgacccttt acaggtcggg atcgctgctc taccctgaag cgtcgccttc ggaggcaaga
    24361 tggccaaggg tatcgaggac gttcagtcgt acctgctcaa gatggacttg cggtacgagg
    24421 agccccgtga ggggatttgg gtacttagtg ggcttgatgg catcgacaag cttgtcatca
    24481 cgcttgctgg tcccgtgctg gtgtttcgcg tcaaagtgat ggagctgccg cagcgcaacc
    24541 gcgagggcct gtaccgcacc ctgctggaat taaatgccag cgaaatgatg cacggcgcct
    24601 atggtgtcga gggagacgcc gtcgtgattt gcgatgcgct acagctcgaa aacctcgact
    24661 acaacgagtt tgccgcgacc atcgacgaca tcacgctggc ggtggcgtcg catcacagtc
    24721 ggctcgggaa gttcaaagac ccgctgccgg cgtaagcgaa acgcgaaaca gcaaaccaat
    24781 aaacccagac acatcaagga ttagcgatgg gatttttctc gcgtttggga tcgctcatca
    24841 aatcgaacct gaacgatctg atctcgaagg ccgaggaccc ggaaaagatg ctcaaccagg
    24901 tcctggtcga tatgaagacc caactggtcg aggccaaaaa gcaggtcgcg gtcgcgattg
    24961 gcgatgaaaa gcgtctcaaa aagcagtggg acgaacaggt cgcgctcagc aaagactggg
    25021 aacgcaaagc gatgctcgcg gtgcaggctg gcgacgatga actggccaaa gaagcgctcc
    25081 tgcgccagcg cgaacacgaa gagctgtcga cgcagtttga gcagcagtgg gtgcatcaga
    25141 aagatgccgt cgacaagctc aaagtccagc tgcgcgtgct gacgaacaag attgaggagg
    25201 cgaagcgcaa gcggaacatc ctcatcgcgc ggcagaagcg agcggaagcg cagaaggcca
    25261 ttcagaacac gatggcgggg ctgtcggata cctcggcgtt cgatacgttc gatcggatgg
    25321 cgcaaaagat tgatcaactg gaagccgaaa ccgaggcaac cacagagctt gcgggtgagc
    25381 tggaaggcga ttcgctgacg cagcgcttca aggcgctgga agccaagtcg accccggcgg
    25441 atctggcgct tgcggaactg aagcagaaaa tgggtcttct gccggcggct tcgtcggcac
    25501 cgcccaaggc tctgccgcag accagcacca ccaagccgga agccaagccg gaagccaagc
    25561 ccgaagccaa gcccgaaggc gatccgacac tcgatcccta acagccacta tccgcgcttc
    25621 ttcccaccag gccgcgatat gatgcgaggt atgaacctct ttgagcgacc tgtgtccgag
    25681 tatatgagtg ctccggtctg cgtggtttcc gccgacgata ccgccgagga tgccaacaaa
    25741 atcatgagcg aaaagcggat ttcctgtctg gccgtggtgg gccgcgatgg aatgccggct
    25801 ggtgtggtgt cgcggtcgga tcttctgcgg gtggcgcgcg cgatggtgtt tgcgacgggg
    25861 agtccgactg cgcttcagct tccgtcgatg tgtgtcggcg atctgatgac gccgcacttc
    25921 tattcggtga cgccgaaaag cacgctgatc gatgcggcgc agatgatggt cgaaaaacgc
    25981 attcatcgcg tgttcgtcgt cgatggaacc aagccggtcg gggtgtgcag taccaaagac
    26041 ctgatgcggg cggtgatgga cgccaaaatc cagacgccga tttccgcgat gatgacggtg
    26101 ccggtggtga cggtggagtc atcgagcctg ctttcggacg ccgtggcgac gctgagccgg
    26161 gcgcggataa gcggcgtggc ggtgatggaa aacggggttc cggtgggcat cttcacgcaa
    26221 gaggaagcgc tggcggcgcg cgatcgtccg ggtcagtctc ctgtcgatga atcgatgagc
    26281 caagcgctgc tctgtctgcc ggtgacgacg ccgatgttcc gggccgcagg gtttacgatt
    26341 tcgacgcggg cgcgccgggt gctggcgatt gatcatcacc atgtccaggg catcttgacc
    26401 gggctcgact ttgcgcgctg catcctgccg ccaccaccga cggtagaagt caaaatcgcc
    26461 taggcgtaga cggctttctt tgggggggct cgtgaaacga agcggtgtat cgatccgggc
    26521 caagatgctt ggcggacttt ggggatctgt ggtcggcgat gcgctcggcg tgccggtcga
    26581 gtttcacagc cgggaagaac gcgagcgtga tccggtacag gaagtgcgcg gctttggtac
    26641 gtacaaccag ccgccgggca cttggtcgga tgacagctcg ctgatgctgt gctcgctcca
    26701 cagtctgctg gagcgcggct tcgacttggc ggacatggga cagcggtttg tgcgctatct
    26761 gaccgattcc tatatgacgc cgcatggatc ggtgttcgat gtgggaatcg cgacagcgac
    26821 ggccatcgac aagctgcgtg cgggctgcat cgccgaagaa gcaggcggca agggagagcg
    26881 tgacaacggc aacggatcgc tcatgcgaat tttgccgatc gcgctgtttt tttaccgtga
    26941 aagtgcatcg gatctgatcg cgcgggcgca ccttgcatcg cgggttacac acggacatcc
    27001 gcgctcgcag cttgcgtgtg gatacgcttg tctgttcatc gcccggctgc tctgtggtga
    27061 ctcggcggcg gtggcgtatg aacgggtaaa tgcgcagatt ccacgcctct attccgattc
    27121 ccactggcaa agcgagctgc cccattttgc gcgcatcctg tcgggacgac tggccgggga
    27181 accgaagtcg accattcgcg gcagtgggta tgtggtgcat acgctggaag cgagcgtcta
    27241 tctgctcttg cgcagcagca cgtactcgca gacggtgctc gatgcgatta acctgggcga
    27301 agacaccgat acgacagcgt gcgtcgcggg cggaatggct ggcgttctgt acggtgaaga
    27361 cgcgattccc aaagactggc tggctccgct tgcgcgacga gaacacctgg atacgtggtt
    27421 ccgactcttt gtcgacaacg tgcagccggc ggattcctaa acggatacgg aatcgcctgt
    27481 cgggcgcgcg gagggattgc ctgggaaccg cggactcccc taggatgggc gaggacagat
    27541 gccgatggat gcaaaaaatc cgcgcgaggt tcttgcttac gttcctccca gccgcgaaaa
    27601 aatccgcctg ctgaccgcgc cgctgcgtca ctttttttcg cccgtctttt tggatctgga
    27661 aaagctggac ttgtcgcggc ccgcgctgtg ggttgggaat cacacgctgc ttggattgac
    27721 ggatgtgccg ctgctttttg aacggctgta tgtaaaacac aatgtggtgc tgcgcagcct
    27781 cggggatcgg ctccacttga aagttccttt ttggggcgat ttcctggtgt cacacggaat
    27841 ggtgctgggt gatccggaag tcggtgcgga gctgatgcgc gggcagcagc acatcttggt
    27901 tttccccggt ggtggtcgcg aagtgatgcg ccgcaaagga gagtcgtatc agcttctgtg
    27961 gaaacagcgg cttggttttg tacggctcgc ggcccagcac ggctacgaca tcattccgtt
    28021 tggttcggtg ggcgccgatg aaagctatcg gattctgatc gatgcaaacg acatattggc
    28081 agggcgtttc cgacggcagt ttctggaacg gagcggactt cttacgctct tgcgcggggg
    28141 cgatttcatt ccgccgctgg tgatggggct tgggggaacg ctgattccgc gtccgcagcg
    28201 ctactacttt ggttttgggg cgcggattcc gactgcgcag ctacacggaa acgtcgacga
    28261 caaacaggcg ctttggcaga ttcgcgaaaa ggttgcgcac gccgtccgcg cgcagatttc
    28321 tgcgctgttg gaatatcgca aaaccgatcg cgcaacctgg agtccgctgc ggcgttttct
    28381 ggctcccatc gacggaaatg acaccgattc ctaaacgaag ggaatcggtc tgctgcgatc
    28441 aaaaagacag gcgagcaccc aggcgaagat acagcggtgc ttggtatccg gtgggctgtc
    28501 cgtagtttgc gtttttcaca atcggatggc catcaatgtc ggtaaggtgc gccaaatccg
    28561 cgatcttacc gtcgacaatc ggcgctacgt tggaatacgt atagtcgtca tcgacattgg
    28621 tgacggcgcg ctgattgaac aggtttatca cgtccgcata gaccgacaga ttcacttttt
    28681 ggctcagcgg ctgctcgaaa ccgacgtgca aatcgaactg gctcaccatc ggggttcggc
    28741 ctccgctgcc gcgcggcagg atgaacacct gactgtctcc gtagataaag tgtgtgccca
    28801 ggacgtttat cggtcgtccg gaatacgccg aaaagctcag cgacgcggtg aggtttcctt
    28861 tgcggatcgg ctgctggtac gttccgatga tttttacgtt gtgcggacga tcgttgggaa
    28921 gcggtccggt gcgattcttc atcatgtcct gcaagtcgaa ctgcgacgaa aagttcggca
    28981 ggttttcatc gaccacccca tcgtaagttc cgggatagtt gcccagcatc cgtgaatagg
    29041 tgtagttcgc caggaggcca aagcgattgg acagacgctt ttgcgccgtc agcaccagac
    29101 cctggtattc ccgtcgcggc ttgggatatt gcgtcgcaat cgatcgatag gcatccagcc
    29161 gcgcttgcgc ccgaccaagc gcttcctgtc ccatctgtga ggagtctccg cttgcgcgca
    29221 gtctgtcgac atcggcttgc agatcgcgca gccgagacgg atcgggatcg acccccggat
    29281 tggcgatgat gtaattggtt ccaccatcga gcgacatgtc ttcgacaatg ttgccaaggc
    29341 cccggtaaat gtagcttgca gacaggacga gatcccagcc aacgtcgtag ctgatgcctg
    29401 cgaccagctc gtccatgtac attcccttga gacccggaat cacattgccg tagcgtcctc
    29461 cgttttggtt gccggcggcg atttcgctta ggttgcaagt gccggttcca tttgtgcgcg
    29521 gaaccggaac ggcgcggcct ccgggttgga gtgctgccgt cgcacaatcg gttgtttcag
    29581 cgccggtaaa gacgccttcc cctgcaaact gacggtcgtt gatggtgagc ggaatcgact
    29641 gataaaagcg tccgtagttt acaaacagct tgccgagtcc cttttgggta aagtcccaga
    29701 ccgctccggc gcgcggagcc cagttgtcat agattccgat ctgccttgat ccgtcagagc
    29761 cgtgcagttc ttgggcctcc cagcgcacgc cggcatttag cacaagaccc ggcaccggcc
    29821 cgacgttcca cgaatcacgc agatacagca cgtagttgtt ggtgaacgta tcggacgaaa
    29881 aactgtttaa cagaatcgtt tcgccgtttg gtcctaccgt cgaatactgc gcaaagttac
    29941 ggagccgttg cccgtcggcg ctggtgcgat acatgacgtt cgcagacagc ccatcactcg
    30001 ggtctccgtt tggtccggaa taggcgcgga agttttggtt gcgcaggtgc tcgaaatcaa
    30061 aaccgagctt gattgcgtgg gttcccagtg cttccaaaaa gtaggtcgct gcgacgagaa
    30121 gctggtgccg ctgcagatcg gtgcggttgt actgaccaaa tccgtttgtg cggtacgttg
    30181 tgataggaca cggattgaaa ctgaccgcat tgcccatcag gtcgcgctgc atttgccgac
    30241 ggcaatcggg gttgtcttca aagtccgcaa gggagtacgg attggtgctt tcggtaagat
    30301 aaagggaaag cggcttgtcg acgtttttgg gcgcgacgtt cagtccctgg aaatggaaac
    30361 cgtacatcgc gtttagctga agcttgcgtc cgagcagctt gccgacgtag cgcagcgatg
    30421 catcgtgcac ttgatcggtc tgctgatact ggctgtcttc gacatttccg gacgtaccag
    30481 accccaaata ggaatacccc gaaaacacct tgggcgacgc gatgtaggaa agcgtcaggt
    30541 tgtgatctgg actcagattg aactgaagtt tggcgattcc gttgtagagc tggcgcagct
    30601 cggtaaagct ctgcgcgctg acttcatcca tttcggtggc aagcgcagcg gagcctcgac
    30661 acagggccgg atcagacaga taggtaggac aggcgtagcc gccgttcaat cgcgcggtgc
    30721 cggtctgcgg attccagatc gcatggcgaa tcacgcgatc ggtttggtac tgagccaccg
    30781 tcggagcgaa gccaatgtaa aaccagattt tgtctttttt gatcggaccg ccaagctcaa
    30841 aaccgatgtc tcccaaaaga ccgtccgctt tgcgccgata ggccagcgct tcgcccaacc
    30901 gtccgacggt tttgggggtg agctgaaacg gctgcatcga cagaaatacg ctgccgtgca
    30961 gctcgtctcc accgcctttg gtgatgatcg aaacgacgcc tccggtggca cgtccgtact
    31021 ctgcttggta gccgcccgtg atgacgttta cttctttgat gaagtactgg tgtagctcgg
    31081 tgccgatgat tccggtgttc ggatcggacg tgttgagtcc gtcgatgatg aagttgtttt
    31141 cgttgccggt ggaaccggaa atggacacgc cgatgtcacc gccttgaccg cgcggtggat
    31201 cgacggttcc gggggccatc gccagcacgc tttcaaaagt ccggccccgc accgcggtgt
    31261 ttttcagcac ctcttggttg atttcgacgc ctgcggtggc actcgcggtg tcgacgttgg
    31321 gagcgcgttc acgaatgact ttggtttcgc gcgcaacttg ctttatcgga aacgcaaagg
    31381 agacggtcag cgtcttgccg tgcgtgatgc gaatgccgct gcgttcttcg actttgtcgc
    31441 ccagataaaa gcgaaccacg tagtcgtcgc cggacggaat ttcggtgata agataacgac
    31501 cgtttccgtc ggtgatttcg atctgttccc cttgcagcgc cggcccggtc acaaccaccg
    31561 tgacaccagg gagcggcttg ccgttcagat cacttgtcac gacgcctttg atctgaccgt
    31621 tttgcagctg cgcgcgcgcc aagtccggcg atgcgacggc acatagaatc accgctggac
    31681 ccatggcacg ctttgccaga ctccgcggca gcgaaacaag tggggaccgt gtacgagacg
    31741 acttcttgct catgtgaacg gctttccgtg accgcgcagc aggaaatgga atgaaccaga
    31801 agccgcgcga tcggcgattt cctaaggcaa aagacagacg acgctaacat ggtcgtgccg
    31861 aaggtggaat gaattgcctt gcttgcaagt tattcccata ccgtggctag ctcataacga
    31921 tatgaggtca actttgaaca aaccatctgc catccgatcg gggacttcgc gggcgcgctg
    31981 ggtccgccct ttgcttttcg cgctttcgct cttgggtgct gacatcgcgc atgctgaaac
    32041 ttcgggcttt ccgatggact cggttggact gcgggtgccg gggcctttgt cgtcgcgcag
    32101 tgcgtcgtat cggctccgcg cgcggctcga tgaaaacacc catgaagtca aaggccaggg
    32161 gacgcttttg ttccgcaatg tcgagcggaa agaagcgcac gagctggtct tcaacctgta
    32221 tcaaaacgca tttaagcacc acgccacgac gttcattcgg gaagccgggg cgcagctccg
    32281 cggcgacgaa atgcccgagc acggctttgg cgcgatcgac gtttcgtcga tcaaaatcgg
    32341 tggcgtcgag ctgattggga aagcccaggt gctagacacg cttctgaccg tcaagctcga
    32401 cacccccgtc gggccgggca gcatcgtcga agtcgagctg gcgtttacca ccaagctacc
    32461 caaagtcttt gcccgatctg gctacgctgg cgactttcac gccgtgaccc agtggtttcc
    32521 caagctcgcg gtgttcgagt gcagccgcga cggtgagcgc tgcggtttcc acgcccacca
    32581 gtaccacggc tttaccgaat ttttttccga tcacggcagc tacgaagtca cagtcgatgt
    32641 gccagaaaag tttgtcgtcg gggcgaccgg ttcgcaggtt gctgaaacgc gcggccaagg
    32701 gcgcaaacag ttgacctatc gggccgacga tgtgcgcgac tttgcctggt tcgccgatcc
    32761 caactttgtc gaatcgaaag cgcgtgtgca ggacgggctc ggggacgtcg aagttcggct
    32821 gctcaaccag ccgggcctgt cgtcagcggt gccgcgacat tttgccgggg tgcgggccgc
    32881 acttctcgaa ggcgaagatc ggtacttttc ctatccgtac aaacaagtga cggtgattgt
    32941 tccgccgcgg gatgccggcg gttctggggg catggagtat ccgcagctta tcaccagctt
    33001 gttaagccca ctgcccgacg gagtgcgcat cattgaaagc gtcgatgcgc acgagtttgg
    33061 ccatcagtgg gtgccgatga tgatagacac cgacgaagtc gaggaagcgt ggctcgatga
    33121 aggtctgaat cagagcttca ccggactggc gatggatcgg ctgttccctg aaggctgcac
    33181 atcggtcaaa ctgcttggat tctgtctcaa cgatcgggat gtcgactggc tgtcgactcg
    33241 cagtgtgctg cgccggatgc cgcttgtgac gccgagcttc cgtctgagcg gtcggactta
    33301 cggcgcgatg acttatgcct atacgtcggc gatgctgcgc agcctggcaa actacctggg
    33361 gcctgatcgc tatcggcaag cgctgcgcca ctacgccgag cgctatcgtt tccgcaaacc
    33421 caaaggcccc gacttcatca gcgcgatgag cgagggcgcc ggcgaggatc tgagctggtt
    33481 tttcagccag accgtgcaga ccacccgcgt cgctgactac gaagtgatgg cgctggaaaa
    33541 ccacaagcac gagctcgcct ttgggctgtg ggactgtccg ccgaagccgc cgattttgcc
    33601 cggtcacgtc gctgacgccg atcgaccgga ctggctgcgc gacataaacg aagccaacgc
    33661 ggctgcttgc gccggcaaag aacccggtcg ccacgagctg agtcctccgt ctgaaaaaga
    33721 gcaaaagaaa gccaaacacg atggcaaagc gggcgaaaaa gacagcaccg acagcgtgat
    33781 cacgatacgg cgccgcggtg agctgatcta tcccgtcgat atcgtcgtca cgttcgagga
    33841 tggcagccgt gacacgtcgg tgtggaccgt cgctgaacag cttgcgcatc ccgaagaacg
    33901 actgaaaatc atgcgctttt tccggcgagc cgccgtcgaa aaagtcgaag tcgatccgca
    33961 aatgaagctg atgctcgacg aaaaacggct caacaatggg ctttcggcca agccgaatat
    34021 gcgtccggta aaccggctgt ttttgacggt gcaaggcctc ttccaaggtc tgctcgagct
    34081 gttttccctg taaccgcgga aggaccaaga gccatgcaag cgaatgtttc tgttcaagaa
    34141 agcggttctt ctgcaaacgc ggggacgctg tcagcctttt cgagcggttt tgcgcgggtc
    34201 ctctctagcc cgctgcttgt tgtcgggatg tggcttttgt cgctcgggac ggcgctgttc
    34261 gcgacgctgc ccgtgcgagc catgctgcgc gatgtgctgt cgctgcgacc gattgccgac
    34321 aaaattgcgc gcggcgaata cgatgtcggc ctgatcgaag tgatgtcgga caacccagcg
    34381 gcaatggctg cggtttcgtc gacgctgatg acggcggcgc ttttgtatgt gcttttgcaa
    34441 gcacttgtgg ccggcggcgt tctgagtcgt ctgagccctg ccgatgcggc gcgtcatgtg
    34501 ccggcgggtc gactgcttgt gcgctcggcg gaaactgcgg gcgcgatgct caagctagag
    34561 cttttgttcg cgctggcagt gcgcaccccg ctgttgctgg tcgcggcggc gctgtgtgga
    34621 accgtgctcg gctggcaaaa agtgggctca ctggcctggg acacggtgtc ggcgcggctt
    34681 atccccgtcg ggctggcgtt tgtgatgttg tggtccctcg cgagcagttg gttacacctt
    34741 gcgcggctgc gccggctcga cgatgaatcg ctgtcagcgt ggcgggcgct tgtgaatggg
    34801 ggcaagacgc tcagcctgtc gccggtgctt tcgggggcgc tcttgctcgc gcttctgtcg
    34861 gttggcggac acggcgggct tttgtggatc gggcggcttg tgactgccaa gctcgacgcc
    34921 aagctgctct tgcttgtggc gtttctggtt cgtcaggggc tttcgcttgt gcggaccacg
    34981 ctgtctttgt gggcactttc gacgacctgc tcgctcgatg ccgcggtttc ttccaggact
    35041 tgcgcacgat gaagcaagct gctttgtctg cgggtgggct gctataatcg gcgcagccta
    35101 tgcgcccgac gcggtccgcc agaaaaaaaa cgagccgttg cgctcccttt cccgtgaagt
    35161 tgtcagcgac gacagcggtg aacaaaggga catttgttgc cgcgtggctt ttggccagct
    35221 tggccctgcc cgaatcgctt tccgcgcagg agcccgcagc gccttttccg gctgcaagtc
    35281 cggtcgcaag cgcaccttct ggcaccgcac ccggatcgat tggttcgttg gatacggctt
    35341 ccgcgacgcc tgcggcttca gcggcccctt cggcaagcac cgacaagctg agcgatctcg
    35401 atcgtcgcac ccgcgagctg cgcgaccagg tgtttcgcag caagacccgc ctgacgctgc
    35461 tgaccgaaaa tctgctcggt tctgcgcaag gtggggccaa gctcgtcgtc gtgcaaaaag
    35521 atcagatggg gcggctgttt caaatggtca aagtcagcta ccagctcgac ggacgcgagg
    35581 tctttgtgcg cagtctggag ccgggccgac cagccgatgc caaagagcag acggtgtatg
    35641 acggcagcgt tcgtcctggc gatcacaccg ttggcgtagc cgtggtctat cgcggcgacg
    35701 gcaacaaagt gttcagctac tacgaccagt acaccttcac cgccaaagca gcgcatcgct
    35761 tttctgtcac cgacggacag acgacgcggc tcgaagtggt gtgtcgtgaa aaaggcaatc
    35821 cgcttttgac ccgcgttgaa gaccgtccca gcttcgactt ccgggtcgag agcacgaaga
    35881 aggccaactg acgtgcgcct gacgccttgc ataccctcat tggctcgtcc gtggctgcgc
    35941 cggcgtcttt tgacgcttgg ctttgtcggg atgtttgggc tgctcttgcc gcaggctgcg
    36001 ccttcatctg ccgtcgccac ggagcaagcc gcgattccgc tgccgcaggc gctcgaccag
    36061 ctcgacaccg cgctgacctc gctcgaaagt tcggtccaag caggacagtc gactccggcc
    36121 aagacgccgc cagaaagtgc agcgcccgtc gccactccgc aacagatggt cggcttggcc
    36181 aaacaactgc acgagcgcag actgttcgcg gcggcagcat cgctgctttc tgaaacgctg
    36241 gttcgctcgc gggagctttc gcaagacgca gacgtgctgt ggcttctcgg cgattcgctg
    36301 tcagagcttg gcgatctgcg tggggccaag cgctatctgg gccagtttgt cgacagtcat
    36361 ccgacggacg gaaaagccct gactaagctg ctggcacttt cgtcgacgct gccggctgat
    36421 ccgatggaga aaacctggct tgggaagctg caagcgattc cagtcgccag cctgcctgaa
    36481 tcggcaaact acgtcgctgg caagctcctg tttttgcgcg gagacctggc cggggcacaa
    36541 aaatcgctgg aagccgtcgt cgagacgagc ccctatttct atcgggcgcg ctacatcctg
    36601 ggcgctttgg cggtgcagca gcagaagctc gacggggccg agacgatctt caagtcgctg
    36661 accgaacgtc cgctgccgcg tcgcgatgat gacgtgcgcg tcgtcgagct gagccacatg
    36721 gcgctcggtc gcattgccca tgagcgcggc gatagcaagc gggcgcacga gtcgtatctg
    36781 cacatctcgc agaagagcaa cctgttcaaa gacgcgatct acgaagcgtc gtgggcggcg
    36841 ctaaaagccg gagattccga gcgggccgag cagtcgctga agctcttgct cattgcctat
    36901 cgcgacgaac cgcagacggt gcttccggtg acggaagcca agctcttgct cggtgatctg
    36961 cttctccgtc gcggcgaacc ggatctggcg tcgccgtatt ttgtgtcggc gcgcacggat
    37021 ctgcgtccgc tcgtcgaaaa actgggtggg gatctgggga cttcgccggc ctttgccgaa
    37081 agactgcgcg ggcagctcgg cgtggccaaa gcaccgcttt cgctttccaa cctggcgccg
    37141 cagtcgctgc ttttttcgct gcgcggcgac gaggtcttgg cccagcttca ggcgatggat
    37201 cgcgagcttt atcagacccg cgtcgcgctc gctgagcaag aggagctgat cactcgcgcc
    37261 gaaaaacagc ggacaccgca gcagcagcag cagcagagcc ccggcgggac ggcagagctt
    37321 gccgagccgc gcaatcgatc gctggcgctg tatcgctatc tggtggcgct gcaccgggct
    37381 ggcggcgatc gtctggatgc gctgcttggt cccgcggcga cgcccgaaga aaaagcccag
    37441 ctcgacgggc tttcgcaaaa acgggcgcaa gtcgggaagg ggctcgccga gccgcgtggc
    37501 ggcaatgatc cgctcgatgg gactgcgagt gcccggcggc tgcatgccga gctgctcggg
    37561 gaagcaccgt ccctgtatct gcatctcttg ctgcggcttt caccgcccca gcgcagccaa
    37621 gctgacgagc ttttgaccaa cctaaaccgc atggcgcaga tcgaacggcg actgcgtgat
    37681 gtgattggca agctcgatcg gatgctcgat gtgcgcgtcg gtgacatgca ggcggcgctt
    37741 cagtctgaac gcgatgcact gcgtggtcag cgggaatcgc tcgacaagct gcaagcggag
    37801 acggcgcagg tcgtcgggca tgcggcgtgg acgcggcttc agcaagtcgt gtccgactac
    37861 aaagagctgc tggttcatgc cgaagcgggt gtggccgaag tcgcgtgggc gcagcggcaa
    37921 aaatccagcg acgagctttc gcagattcag gccgaccaag cgcgggagct gcggatgctc
    37981 gacgatgcgt ttgcgctgcc cgaagacaac cgccctgccg caaagcccgc gcagtcgccg
    38041 cagcaaagcc cgcagaatca gacggcaccg aatgtccccg cgatgcctgc ctcggcgatg
    38101 cctgccccgg cgaaaagcga agcggctgtc gtcgccaaaa acgaagaaat cccgcttcca
    38161 gccgatcttc ccgtcgaact gcaagctgat taccgtcgct atttcgaggc agcccgcagc
    38221 tacaagcaca cgcttgatga agtgggtcga gacggctaca aagttcgtcg cagccatctg
    38281 aaaaacgaat ttgccgctcg tctgctccgc gaggaagcgc gtgaaaagca gcttcgcagc
    38341 gaagccatcg tcgagctgga aaacttcctc agccgctatc cgcagcatcc gcgtcactcg
    38401 cccgatgcga tgttccgtct cgccgagctt ttgtttgagc ggaccagcga gacgtttatc
    38461 gggcagtcca aatcgcagag cggcgaaatg gtggcgctgc ccgactacac gccgtcggtc
    38521 gatctgtatc ggcggctttt gcgcgacttc ccgcagtatc gcaacagcca tcttgcgacg
    38581 tatttgctcg gctactgcct cggtgaaatg gatcacgacg aggaagcgcg ccaggcgttt
    38641 ttgggtcttg tgtgtgcaaa caagtttgcg ccgcttgcga cacctgcgcc gccggtgggc
    38701 cgtcgcaaca agccgcctta tgacggctgc gagccgatga aatcagacgg caagctgctc
    38761 gcggaaacct ggacgcggct cggtgaatat cactttgatc ggggcgagct cgacgcggcg
    38821 atctttgcgt atcagcaagc gatcccgttc cgcgactcgc cgtactttga caaagcgctc
    38881 tacaagctgg cgtggtcgtt ttatcgcgcc gatcgcttca ccgacgcggt gaagcacttc
    38941 gatgagctgg tcgtgctcgc cgacaaaaag cagcttggcg aaccgggcaa aaaagaaggc
    39001 ttcgcgctgc gcagcgaatc ggtgcagtac ctggcgcttt cgttcgctga gcgagactgg
    39061 gatggcgacg gcaaagacga tccggtcgcc ggactttcgc gtgccgaggt gttctaccga
    39121 ggtcgcgagc gcgagcccca cgtccgcgaa gtcttccagc ggatgggcga cgtgctgttt
    39181 gaccgcaccg aatacggacg cgccgccgaa gtgtatcgga tggcgattgc gacgtggccg
    39241 ctggcaccgg aaaatccccg tctgcaagac cgcgttgtcg tcgcgatgga aaggctgcgc
    39301 aacttcgaac aagccctgcg tgagcgcgaa cagcttgcgc ggacttatgt cgagggcagc
    39361 gagtgggcca agcagaaccg cgacaacgac gccgcgctgg ctgaaggctt gcagcttgcg
    39421 gatctggcgc tgatgaacgt cgttttgcgt cgccacgaaa gcgcgcagaa gctgcgggaa
    39481 gaagcgcagg ccaaaaacga tcccaagctg cgcgcccaag ccgtcgagga ctatcgtcaa
    39541 gcggcgtcgt cttacgagac gtatctaaag cagcatccaa acagcaagga ccgctacgaa
    39601 tacagctact acctggccga gtcgctgttt tttggcgaaa agtatcccga ggctgccgag
    39661 gcctacgaga aggttcgcga ggccgatccg aaaggcaaat acgtcgaaga ttcggcctac
    39721 ggtgcgatca agtcgcgtga agccgtcgtc tccgcgctgt acaagcaagg tggtggaatc
    39781 gagccgccgc tgccgcaggt tggcaaagtg acaccgcctg tgtcacctct caatctgccc
    39841 gacgaagtgg cgaagctgca atacgcctat gaccgctttg gcgtgctttt gccggattcg
    39901 cccaaaatcg cgctgttttc gtacaaagcc gccgaaattg acttccgtta tttgcgtttt
    39961 tcgcaaatgc ggctgcggat ggccgatatc gtcgccaagt tctgcaaaga cgagcgcgcg
    40021 gtcgatgccg gcaatgcgct ccttgtttcg tacaacatcg aaggcgatct cgaaaagctc
    40081 gaggagtgga cggcgcggct caaagacaaa ggctgcggtg gttcgtcgca ggttgcgcag
    40141 acccagcttg gcagcatcaa aaaactgtca cagcaggtgc ggttcaaaaa agccgagcag
    40201 ctgcttgcgc agaaacgcca tgccgaagcc gcgtcgctgt ttatcgaact ggtcggccaa
    40261 gatccgcgcg gccccgattc ggacaaagcg ctgttcaatg cggcagtcgc gcaagaaagc
    40321 gacaaacgct acggcgcagc gacggaaacc tatgaacgaa tcgtgcgcga gtatccgcag
    40381 agcaagcttg tcgatgaagc gctgttccgt gcggcggtca atcaccagcg cttctttgat
    40441 ttcgacaaag cggtgacggc gtatcagcag cttggcaccg atccacgctt caagggatcg
    40501 acgcaccgtc acgatgcgct ctacaacgcg gcgctgattg cggaaaacga tcagcgctat
    40561 gcgctcgccg ccgacctgtg gaagcagtac gcgcaaagcg acaagacttc tgccgaagaa
    40621 gcggcgacgg cgtatttccg ggcggcgctg tcgattgaaa aactgggtcc agcgtcgcgc
    40681 gccgaacaag agctgtcgca gtttgtcggt cgttacggcc aaagcgccag ctctctgccg
    40741 caggttgtcg aggcatacgt ccgtctgtcg cgggtccagc aaaagcaggg caatccgttt
    40801 gatgcgacgg aaaatctaaa gcgcgcggcc cagcttgggc agaaattgcc gccgggttcc
    40861 gacgcagctg agtttgcggc ggaagcggcg ttccttttgg ccgagcagaa gctgcaagaa
    40921 gtcgagcggc agaaaatcgg cggcagcggc aaagagctgg aagcgtcgat cagcgcgttt
    40981 aacaaaaaag tgcaggagac cgtcgtcgtc tacgacaaag tcctgtccta taagcgcgcc
    41041 aatcacacgc tcgcagcgta tttccgcatg ggctacgtgt tcgagctgta tgccaaggcg
    41101 tttttggccg cgccgtgtcc gcccgaggtc aagcggctcg gggccgaggc gtgcgatctg
    41161 tacaaaaaca agatcgaaga aaacgtcgcc ggcatcgagg aaaaggcgct tcagcgctac
    41221 gccgtgacgc tggaacaagc ggcgaagctc ggcgtcggca accagtggac caagctcgcg
    41281 cggcaacgtg ccaatgcgta tcggcccgat gcctatccac tcgtgaaaga cgaacacgtc
    41341 gccaaacagc ttgtgcttgc cggaccggtg caagcccaag acggcagcga gctggcgaag
    41401 cttgcgaaag aagcgcgcag cgcgatgtat tcggggcagt acgaaaacgc gattgtgctg
    41461 gcgaagcttg tgctgagcaa agacgaccac catgtgccgg cgatgctgac gctggcgacg
    41521 tcgtattact ttttgggcaa gcgcgagctg tcggcggcga tcgtcgggat ttcgcagacg
    41581 attgacgcaa acaatgccga agcatacgtg ctgctcggct acctggcgct tggcaaagac
    41641 gatcggatcg ccgcgacggc agcattcaaa aaagcgaccg agctcgatgc tggaatgggt
    41701 ctggcgtggc acaacctgtc ggcgatgtac cttctggcga aaaactacga gcaggcgctg
    41761 tttgcctgtg agcgctcgac ggtgcttctg ccgggtcttt cgggaacgca gcttaacttt
    41821 ggatcggcgc tgcgcggcat gaagcgctac atcgacgccg acaccgcgta caaaaaagtg
    41881 ctgtcggcag agccgcagaa caccgacgcg ctgttcaatc tggggatttt gtacattgat
    41941 gcgccgtcga tgccgggctt tgactcggtg agccagcgga tgatcgcgat tcgttacttg
    42001 gaatcgtatc tggatgcgat cgggccgcgg ggtccgcgcg acgaatctgc cgaggcgtac
    42061 atcaaagatg cccgcgctca gctggagcga gagcagcggc ggcagaagaa aaaacccagt
    42121 cccacaccgc cggcggaggg atcgccatga gatcgcacgg ttggctttgc gcggcacttt
    42181 gcgcgctggc gtttccttct gtggcgtcgg cgcaaaagac cgaaaccgcc gatcaatcat
    42241 ccgttccggc agcgaaaccg gcagccccga cggatttgac agcggcggga ccgcctgaaa
    42301 aaccagccga tgcgggcaag gccagcgaaa ccgctcaggc aggtgatgca gcccaagcgg
    42361 gtgatgtgaa aaaaccgatc gacaccgcgc cgaaaccagg tgaagccggc gcgagcaaac
    42421 cggctgccga tgccgcatcg atcaaagtcg aacgcaccgc gactggcgcc aagctgttcc
    42481 gcatcaccga aggcctggtc gtcgaaggcc agatgcaaaa accggcggca ttttacgtgt
    42541 tacggcgggc cgccatccac tacgactact ccgaactggg caagagcttc ttgccgaaaa
    42601 ttggcgccgc ggtaaaggct ccgccatttt aggtttttga cgatgaccgc tcttcacgac
    42661 ggatctgctg aacatggcga acgcggctcg ggtcgcgctg ccgatcgggc gcttcggctc
    42721 ggacttttcc ttgcgggcaa gccgctcgag gaacgcctgt ttcgccagcc acgcgacata
    42781 agcctgggca aaaagaacga ctgcacggtg atcgttccgc cgaccgaagg gctgtcggac
    42841 agcacaacgc tgtttgtcgc cggggaaaaa ggctgggtgc tccagtttgg cgaggagttt
    42901 aaaggccgcg tcgcactgtc ggacaagccg gtcagtcttc agcagctgat cgacgatggg
    42961 gatgccatcg cccacaagca gctgtggcgg gtgccgcttc caggccatgc caaaggcgag
    43021 ctgcgctttg gtgatctgaa gatcctgttt cagctggtgc cgccgccgcc ggtggcccca
    43081 agaccgcagc ttccgtcttc gcttcgcccg cagcttctgt cgacgctcga caagcgcttg
    43141 gcgcagattg cgattgcgtc gtttgtggcc cacttttcgt ttgtcgtcta tctgcgcgcg
    43201 atggagcggc ccaagcccgc agcgatcgag gaaattcccg agcgctttgt gaagatgctg
    43261 gtgcccaaaa aggtcgagcc gcccaagccg ccgacgccag ccaagaccga agcgaagaag
    43321 gccgacgaca agaaagcgga cgacaagaaa aagaccgaag tcgccgcaaa cgacaagcca
    43381 gcgacgcagc ccaaaaagga agaagatccc gctgcggcgg ctgcccgtgc cgctgagcag
    43441 cgcgcgaaaa tggaacagca gctctcttcg atgggcgtgc tcaaaatgct gacggcgaag
    43501 gggcccgatg gggcgctcgc tgatgcgctc aaagatggtt cgggcgatgg cgatcccgac
    43561 aaagtcttcc gtgaagtcgg cggcgtcgga cctgtcacgg gtcaaaccgg acttggcacg
    43621 gttcgcggcg gcgatggcac cggaaccagc cagagcattg gttctctgac gacaaccggc
    43681 ccgcgtgatg gggtctctac cggcgaaaag acggaaaaga aagtccgcgc cgtcgtgcag
    43741 gagtcctcgc cgcaagacct cgatccgggc gatctggacc ccggtcaggt cgcggcgaag
    43801 atccgtcagt accgcggcgc gatggtggct tgttatgaag ccgcgctcaa acgcaacgca
    43861 acgctggccg gcaaaatcac actgcgcttt tcagtcagca gcgccggcaa agtcagccac
    43921 gtcgagatcg aagtcgacac catgcacgac gatgacttcg ccaagtgcat cgtcgaacgc
    43981 gcccagtcgt ggcgcttccc cgcgcccaaa ggcggcggcg aagtgcagtt tgcctacccg
    44041 tttatcttcc agtcatcgaa gtaaccgggc acgcccggct acacaccggg cacgctcggc
    44101 tacacaccgg gcacgctcgg ctacacaaag aagtcgggga acagcggctc gccggggatg
    44161 ttggcgtagt ggtcgaggtt cgtgatgcct tcagatgcca gcagctcgtc gtcgatgaag
    44221 aagttgccgg tgcacgatcg gctggggcgg gtcaggatgt agtgcgccgc gtcgcccatg
    44281 atctcgggct tgcgactgcc acgcatcacg gtttcgccac cgagcaggtt gttaatcgcc
    44341 gatgtcgcaa tcgtggtgcg cggccacagc gcgtttacgg ccacgccttg gtcgcggaac
    44401 tcttcggcca tcccgagcac gcacatgctc atgccgaact tggccatggt gtaggcgacg
    44461 tggggcgcaa accagcgcgc ttccatgttg agcggcggcg acaggttcag gatgtgcgga
    44521 ttttgcgctt tgagcaggtg gggcagacag gcctgcgaac acgcgaacgt gccgcgggtg
    44581 ttgatctggt gcatcaggtc gtagcgacgc atcggggttt gaagggtgcc agtcaggctg
    44641 atcgccgatg cgttgtttat gaggatgtcg atgccgccaa acttttccac tgcttggttt
    44701 acggcggcga tgatttggtc ttcgtttcgg atgtcgacga cgcaggggag ggctttgccg
    44761 ccggcttttt caatttcttc ggcagcggtg tagatggtgc cggggagctt ggggttgggt
    44821 tcggcggtct tggccgcgat cacgatgttg gcgccatcgc gggcggcgcg gagtgcaatg
    44881 gcgagtccga tgccacggct ggcaccagtg ataaacaggg tcttgccgga aagggagcga
    44941 gtctgagtca tgccttcttt ggtatcacgc ggccttcgtg gcgcatagct tgattgactc
    45001 gtcgcgggtg tggggggaca gtgtggggga tgttggcgct gggaattgtt gggtttagtg
    45061 gttcggggaa gacgacgctg cttacgcggg tgattgaggt gctgtcggct cgtggggtgc
    45121 ggattggtgc ggtgaagcat gcgcaccatg gctttgagcc cgatgtaaaa ggcaaagaca
    45181 gcgatcggat gcggcgggct ggggcggttc agacgctggt gttttcggca gagcggtggg
    45241 cgctttttac tgaacagaat ccgccgctgg ctccaacgat accggcgctg gttgcggtgc
    45301 tcgatccgcg tcgcatcgac ttggttttga tcgaaggact gcgcagcgcg gattatccca
    45361 agattgaagt gcatcggcct tcgctgggtc atcctctttt ggcgcaaacc gatccgcggg
    45421 tgattgcagt ggctactgac gatctggatg cactgcgcgg gctggggctt ggctgtcctt
    45481 tgctcgacct aaatgacccg caaaaggtgg ctgattttgt gggaagctta gggcaaaaag
    45541 ggggtttcaa gatgagtgag aacacgacgt cgcaagcaag cagcaaggcc cagttcgagg
    45601 agcttcgtgg caagttcgag gagcgactgg gtcagctgag caagcaaatc gacgagctgt
    45661 cggagcgttt ccaaaaaggc gccaccgatg ccaaagccag cgtcgaagcc gacctgcaca
    45721 agctgaaagc cgagcaccag gctgaatacg agcagttcca gaagctgcaa aaagtcgcgg
    45781 aagagagctg gggcatgctg caaaagcggc tcgataccat cgccaccgag atgcgttcgg
    45841 tggtgggtca agcagtcggt caggcggcga gcgcacttgg gattgcccac agtgaacaga
    45901 cagacgccac ggcccaatcg caagctgccg agccagccgc cccggctgcg ccttctgccc
    45961 cggatgctgg caccgattct gccccttccg cgaagcagta agttgcacgg tcccattcgc
    46021 tgcgccgcac agctttcttg aatgccagcg ggttgccacg ataaaaccct cttgctgcct
    46081 tttcttttcg aggagagtga tgtcgttttc ccgtcttgtc cgcttcccca aagccgcggc
    46141 ccttacgtgc gcactttttg ccgcctgtgt cgaacagagt gatgacgctc cgagcaccga
    46201 gtcgctgcaa gcggccaaag agcacatctt gtcggctgct ccgacgccgc gctttgcaag
    46261 tggcgcggtt ctcgaaaacg ccaaaggcgg acgcatcgtc tatctgggcg ccgatgtcga
    46321 cgtcaacgaa gtcgcccccg gccagtcttt taccgtcacc cattacttca aggtcgaaaa
    46381 agcactgggc gacggctgga aattgtttgt tcacatcgcc acgccggaca agcgactgca
    46441 tcacaacgcc gaccacgttc ccatcggcgg caagtatcca gtctcgatgt ggaaagacgg
    46501 cgagatcatc cgcgacgtgc agaagattcc gctgccgcag aacttcacca acgacaagat
    46561 ggcgatgttc gtcggcttct acaaaaagaa cgaacgactg gcggtcaagt cgggacgtca
    46621 ggacggcaaa aaccgcgtca tcgcgctgga gcttccggtc aaaggcggca cccagctttc
    46681 gtctcagcac aagctgcaag tccggcatct tggccccggc cagtccatct tggtagatgg
    46741 caagctcgac gaacccgcct ggaagaacgc ggtgtcatcg gggctgtttg tgaacaccat
    46801 gaacggcagt cccgtggaac aacccgcgca ggcccggctt ctgtgggacg agcagaacct
    46861 gtacatcggc tttgagtttg ccgaccgcga tgtctggggc acgctggaaa aacacgacga
    46921 caagctgtgg acgcaagaag ccgccgaggt gtttttggac ccggacggcg acggcaagac
    46981 gtatgtcgag ctgcaagtca gtccgcgcaa cgtgacgttt gattcgtggc tgcctggcta
    47041 ccgtcagaac gacaatgcgt gggattcggg catgcagacg gcggtgaagg tcgatggcac
    47101 gctcaaccag cgcgacgaca aagacacgtc gtggacggtc gagatgcgga tcccgatgag
    47161 cgccgtaaaa ggtcgactgg aaaccgtcaa aggcgtgccg ccgcaagtcg gaaccgagtg
    47221 gcgggccaat ttcttccgca tggatcagga aaacggcaag ccgcagaccg cttcggcgtg
    47281 gtcgccaccg cttgtgggtg acttccatgc gctcgacaag tttggctcgc tggtatttgc
    47341 cgatgccggt ggcaatgtca cgccgccgcc tgcgcctgtt ccgcaggccc cggcacagtc
    47401 tgctccgcca gccgtcgctc ccagcacgga ctcgccgggg ggaccgcaca agctcaaccc
    47461 actgctggag cacgcgatga agggcgatga cagtcccgcg cctgccccag cccctgcggc
    47521 aaaacccgcc gcaagttcac ccaagaacaa gtaacccggc tcgctgccgg acccgcacga
    47581 tcaaggagtt ttgaccgatg agccatcaca cgcatcccaa gttcgagctg atttatttcc
    47641 ccatccacgg tcgcgccgaa gtgctgcgtc tgacctttgc actggcccag gccgagttca
    47701 ccgacgtgcc ggtcacggac tggatgacgc tgaagccgca gatgccgctt ggccaagttc
    47761 cggtgctcaa ggaaacccac ggtgatgagt cgtggatgat tccgcagtcg ggcgcgatca
    47821 ttcgccacct gggccgcgtg ctgcacctgt acggccaaaa cgagcgcgaa cacaccatct
    47881 gtgactacgt gctggagtcg gcgatggact ggcgcgccaa gttcatcccg gtcgcttacg
    47941 gcaagatgta cggcacgccg caagacgtgc aggacaagta ctggaacgag ctgtgcgacc
    48001 acaacctgcg gctgctcgac aagctgcttg ccagctcggc caagacgggc gagtggttcg
    48061 cgggcgccaa gccgaccatc gccgatgtgg cggtgtggga cacgatggac agcaacctgg
    48121 ccaagcgggc cgacgtgctc gacaagtatc cggcgctgcg cagcttctat gaccgcttcg
    48181 ccgcgctgcc cggcatcgcc gcccacctgg gcagccgtcg tccgtctgaa caccgccaag
    48241 cgtaagctcg ctgctcgttt catcccgctg ccttcctgca cccacccgga aagacccaag
    48301 tcatccccgc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagaaa
    48421 cgagcggcag ggaagagtta gatcatcctt ggcgcaaaga aacgagcggc agggaagagt
    48481 tagatcatcc ttggcgcaaa gaaacgagcg acagggaaga gtt
//
LOCUS       DAORGN010000012        45672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_269_length_45672_cov_6.260977, whole genome shotgun sequence.
ACCESSION   DAORGN010000012 DAORGN010000000
VERSION     DAORGN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 45672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45672
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_45672_cov_6.260977"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..717)
                     /locus_tag="PKL17_02655"
     CDS             complement(142..717)
                     /locus_tag="PKL17_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK43654.1"
                     /translation="MTIQRLRFRLRKLREHGEELTEESGELNLVPYLDIVMNIIMFLL
                     ATVTFQASLASININLPTSSVVTTQNSAPPKPELNLTVSITEKGYTLATSGAVLFRGF
                     VLTPQGVQQTTSELPTVPLGPDGKHDMAALAQILVQLKNTYPDEERAIITATPQIPYE
                     LLVSTMDAMRQTAGADPKALFPGVLLGAGVN"
     gene            complement(735..1475)
                     /locus_tag="PKL17_02660"
     CDS             complement(735..1475)
                     /locus_tag="PKL17_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK43655.1"
                     /translation="MQQLKEFFEQGGFFMYVNLLCSVVVVTLIVDRAMFFLGKGAVNA
                     RAFLEQLRKLLSAGQLEKAQRLAQSSDAPIALVAKAGLSKLKNGEAAVSTAIEEALTD
                     ATPELKKRIGVLWSLANIATLLGLLGTINGLIGGFAAIGKAAADQRSSLLATRIAEAM
                     NNTWLGLAIAASCMIGHLFLSAASKSKQQELESFALRLENLLFDLIANPPAPAAEKES
                     RDGGRGGSRRDAEKNRRARDVEDEEDND"
     gene            complement(1500..1847)
                     /locus_tag="PKL17_02665"
     CDS             complement(1500..1847)
                     /locus_tag="PKL17_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43656.1"
                     /translation="MSELIPPSVFYKLDTRDTTSVVTTYYAAYRGYENGKGGKAVLFT
                     LNRDFDSQVVKAKVLFVIDVAKDQSVRSYVSERIADVIADEVLPGKPDFQEVDTGSYD
                     QIFAFLGDMLEMQ"
     gene            2054..3241
                     /locus_tag="PKL17_02670"
     CDS             2054..3241
                     /locus_tag="PKL17_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK43657.1"
                     /translation="MPIVRTIVFVSIMTAAMVGLHYYVYLRVTRGLALGPSEQRILKA
                     VLTALFALLWLSFPLTRLVSRSAALPFMWVGYIWMGTIVLSSVVFGLFDLSRFVAGLL
                     SPAVPIDESRRALIGRLFDVAALGGATLVSGCAVALGLRPVVVKRIEVALKKLPAELS
                     GFQIVQLTDVHIGPTLDGKWLTQVVEQVNAQNPDAIVITGDLVDGSVAQLGQHVAPLA
                     NLRARHGVFFVTGNHEYYSGVDSWLAELSRLGIRVLRNEHITLRPSKLAGEGASDLGI
                     DLVGVDDFHADVFPGHGPDLPRAVKGRDPSRPAVLLAHQPAAVMEAAQHGIDLQLSGH
                     THAGQMWPWGYFVRLQQPYVYGLHKHGDTQIYVSAGTGYWGPPMRLASEAEITCVILS
                     RAG"
     gene            3309..3686
                     /locus_tag="PKL17_02675"
     CDS             3309..3686
                     /locus_tag="PKL17_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43658.1"
                     /translation="MTQTKLLALSALLASLLASAAHGDEPAVKADAAIKAEAPATDRA
                     ARRAELVKEVAAARAERRQELRTGERPAAPASGSERIAEAQKRREAQSTRRDEGRMSV
                     RELQREHREELREHRRGMIRRTL"
     gene            complement(3712..5238)
                     /locus_tag="PKL17_02680"
     CDS             complement(3712..5238)
                     /locus_tag="PKL17_02680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNK43659.1"
                     /translation="MNQPNARWLDGLSPAQKEVATYATGPQLVTAGPGSGKTKTLCAW
                     ISELLAQDLCRPKEILAVTFTRRAAGELRERLSGLLGPLADALLIGTFHHFSLALVPL
                     VSPLRLAEESERLQFVEEARVGSGCRKSPASLLSLLSLRKGQHPDYPDRLARGDLDVS
                     PDELVVFQRYAALCAQHNVLDLDDLLLRALASLRSGLTSRLFRFVAVDEYQDVSAVQR
                     ALTVELGKTATVLAIGDPDQAIYAFRGGEVRHFRDFVTDFPAAQVRYLCENYRSTAEI
                     VGAASAVIVANPGRTAPPPVPCRPAGGKIQLLRSPSLRSEAVRIVHEIERLIGGSSLS
                     LHDRRLTDLWRAGSYGFSDIAILTRTIARADAVAEALHTAGLPFSRPSKAQTPLSALP
                     DASPPPNPIWECDHLEDQRIAVLTLHGSKGLEFPVVFVVGLEASVIPGPGRDDDQREE
                     ERRLFFVGMTRAKDHLYLSRIEADGCAPCPFLSQVPAELTESEAPKKPKPQKPQLRLF
                     "
     gene            complement(5235..6557)
                     /locus_tag="PKL17_02685"
     CDS             complement(5235..6557)
                     /locus_tag="PKL17_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HNK43660.1"
                     /translation="MELLLDLHVHSRFSRAVSADMDLGHMYQWAQRKGIDIVATGDVL
                     HPAWRAEMRAQLVPADDGLGHGLYELDPGLREPLDQAIPRSCRRKVRFILSGEISHIF
                     KKDGAVRKVHNLIVLPSLDVADQVADRLLRLGYNLHSDGRPILGMPCRDFLELLLSTD
                     AAAYLVPAHIWTPHFSVFGSQSGFDRLSDCFGDLTPHIFALETGLSSDPAMNWRLSAL
                     DSLSLVSSSDAHSPQKLGRELTRLDIPSPPTFVDVRRALEVCSPDEFLGTIEFFPEEG
                     KYHLDGHRDCGVRLEPGERAALAGLCPRCGKPVTVGVLSRVLALADRPEDNTYRPPRA
                     RPFDSVVPLTEVIADVLGKTAASDAVQRLYFKLLDQVGPELSVLRTAPPAELARAGDS
                     LLAEAILRMRRGELRIAAGFDGEYGRIGLLDPSERSQKSTSRQMGLFG"
     gene            6701..9160
                     /locus_tag="PKL17_02690"
     CDS             6701..9160
                     /locus_tag="PKL17_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43661.1"
                     /translation="MPKFRAGAPDLVVSPDGKWAAEREGGELRVYNLTLPDGAPNRLG
                     PCETQRQEDRPGRIFFVQNDRLMNLSVDDGGESGSAVVVAELLQLPMLAKIGRSVRIP
                     GTQRILGGGPGGVVVAPTGAGAELVVPRESDLVVYKLFVRGEALSAGHTPDKRILIEQ
                     RSGFEIWDAQTRRAMAKLALNTRQAALQLGFLGDGRMVWALTSAMPMRIEVFRASDGL
                     RFFELERPGRALCVESAQGRLVIGFEDDDTISFLDFDVTTRALRQVRLPPDCKRPLAF
                     VVTPSSKLPELLVRLDETEEPLLRLPLTRITAKEPAPRPGTETRGSSRESGATAGSKT
                     SARPDGQTRTTNRAMRDARPESRLIRRLSEAESREAEGKSSSSAAIDPVPATSTPLAS
                     DGEPDENRLVDLEGGSDTDEPQAVPLVPEISLGGPSQKTPPPLAPVQATPPPLRAYDA
                     QRSPAAWQWELARWAQTCQLVADATPPPSSGPLHELSARLQLPQAAQRVLGLLYATAT
                     LLGTRPHGMRPVELAQALRGQCEEPDVLAEVLPSSLLRSLDLLVQRPDGRLRLRREVS
                     LRLAGTPDPDVQWPQTVAREMLQAGVYLLDGPCVQKPARLLGRAVLRLDGLVEPTPHK
                     RLPALLRRALLQDAVLVVDGLAGLSYPAFSAPTVLAELQPQLVQPRVPVVIWAMPEAA
                     AALGLQGRRLTELSIVREGPAPNFPSSALPQGVLFRPPSPAAKAAASPRAIGQLVIAQ
                     VSDRRAALVLGPSANAEAYARAAYLAARDGALLLLEAELTPPRVALLAMLLKQIPVVV
                     AATPPGGPDNPWPPELRPYAF"
     gene            complement(9157..10548)
                     /locus_tag="PKL17_02695"
     CDS             complement(9157..10548)
                     /locus_tag="PKL17_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43662.1"
                     /translation="MSNGRHFTPPTPAFRPVDPFAPIVPRSAERVPSCEVTRPLPSKP
                     RPVRVDTSSRDQAHSGQKPLPPERPNAELPAGVAAASQSAKADKKSSATTPSKADPAA
                     AVKPAEAAKAAPVKVSAAKEEAAAPSSKSPAKAEKVAAKPVEKAAEKAADKTADKAAP
                     KSTAKVAPAEKTAVAEPAKKASAKTEAKADSAAPARKPSKSEPPAPASTKSSSKKAAP
                     VVVEDAPEVVEEPVIRAAKSSKSSKASPVAKAEPVESSKSSKSEKSTATPKSAKSAPV
                     VEAEADSKPAKSAKSAPAAKAAEPAKKSGKSEPAEPAKAEKAGKAAKAEKPAAEAKPE
                     KAESDTKAKKTEAAARPAKSAESDAPASKASAKSAAKAESEAPAKATAKSSKSAPKTE
                     EEAEPAPAKKAKGGKKDGAARKLGPDEELRICEWEKCGKEFVVKVSGRTAVRRFCSGT
                     CRGRASEARTGKR"
     gene            10820..11551
                     /locus_tag="PKL17_02700"
     CDS             10820..11551
                     /locus_tag="PKL17_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK43663.1"
                     /translation="MSGDEDSDCIRVEGLRKRFAESGGAESEVLRGISLAVRRGELVG
                     LTGRSGSGKSTLLHIMGGLDRDYQGKVWLLGEDLAKLSDRALASLRNRHIGFVFQAFH
                     LLSHLSCLDNVLLPNAFAVDPLPRAEAEKRAKAALERVGLVEKMASRPTELSGGQKQR
                     VAIARALLFRPEVLLCDEPTGNLDDITGQQIISLFGELNRDGLTMVLVTHEPRVAEAT
                     TRLCRLQDGLVISDGNSGTSKESLP"
     gene            11548..12930
                     /locus_tag="PKL17_02705"
     CDS             11548..12930
                     /locus_tag="PKL17_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK43664.1"
                     /translation="MTFRQAQSLLFHSLRRAPRAFLLSVFGIAVGISVLGFFLALSLG
                     MQKRVLQRIFPSDRLEVVPPKAAVDEGAMGALSALLGGPKGLGDDVIRELRAHPDVQA
                     VYPRLRVAFPVRGWGGERIVGRPIYTDLPIDGIDPAAVTEATSPWAFKDYVDDNKNPF
                     CTEDKNCPQGWFCSWDKNKCEPPVPVIISPTLLEFYNGSFARMYGLPRITGFLVNQLR
                     GLLATAELGKSFVSRREVGKPRQRKLVLVGVSDRAQPFGLTVPLPYVQRWNAEYAGEK
                     AASEYSSLTVQVKPGKSSTRVVERIRKLGFALEDNGAEQAGLAVLLMTALFVLVSLST
                     LVVATLNVAHTFFRAIAERRHELGVMRAVGASQFDVILLLLSEAACIGLCGGGLGIVV
                     ARIAGFGVDYLSRTALPDFPFKPDSYFWFSPLLLLSLMGFSVAICLLGALFPARAAAR
                     LSPAEALTAK"
     gene            12942..13142
                     /gene="yacG"
                     /locus_tag="PKL17_02710"
     CDS             12942..13142
                     /gene="yacG"
                     /locus_tag="PKL17_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNK43665.1"
                     /translation="MSTLHCPICKKALPDDPNPPKKEARSYGPFCSARCQKIDLGNWL
                     GERYAVPGDPTVEEPEPRELPS"
     gene            complement(13212..14378)
                     /locus_tag="PKL17_02715"
     CDS             complement(13212..14378)
                     /locus_tag="PKL17_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43666.1"
                     /translation="MRKAIWTLCLGLIFPSLGCNTPTPEAGPAGMAGTKGEKGEKGDK
                     GDPGDKGLPGSTGPAGAKGEKGDPGAAGAKGDKGDTGATGPAGPMGVKGDPGAAGAKG
                     DKGDPGASGPAGVKGDKGDPGPQGPTGPAGAGGAYTEESPSFAGFTATTYTGSVTNGR
                     AGMHALCAAAFPGSHLCHAAEYLVSNSGQTPPSAGAWLDPSTGDGKGIQSFGSPKFGR
                     YPHDYTCNSWSSTAGTTYGSTWVNVAAGITRGSDCTSSRPLACCKGIAKVRFSGFTTA
                     TTAGDAGGRHKMHALCAAEFPGAHMCHATEYLRTSSATTPPSDGGWLDPSGNLSGDIT
                     NTGVPDHARLPASYACNDWTSTAGTTYGSTWVNANGGVSRGSDCTTKRAIACCE"
     gene            complement(14478..15008)
                     /locus_tag="PKL17_02720"
     CDS             complement(14478..15008)
                     /locus_tag="PKL17_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK43667.1"
                     /translation="MESKAVLSFADLVALLQSENVPCQGDEANQVVSFPVKNGPLDSE
                     MIIRWEKQLLLAQFAVAFPYKIPPQHMQLTEHAVALCNHRLIMPAFGIDHDNRLLYYR
                     VVVPRADDGGLSYDHLFRLVSTTIDTMIDFYELLGRVVLQGEAVEPLLEELGPKLKLA
                     VAPSEPSAPPSGQLPS"
     gene            complement(15086..16750)
                     /locus_tag="PKL17_02725"
     CDS             complement(15086..16750)
                     /locus_tag="PKL17_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43668.1"
                     /translation="MTRLRPVTLCFPPHWYYAAVPADLLYTGSFLRSHDIPVRAIDLS
                     AGLLHHHLRSVPGFAALQKRETYLSASAYATAGQQVDDALLAVSLRYGCEYGFPALRF
                     PSVDIEHVPTALQVGLSLSRNPALPILQRAISTLVKDDPVYIAVALVHPDQLPQTLVL
                     GRLLRAAGYRGFLCMYGAHEDVLSPEDLLDDLVPAPGQPLHHFFADFDGAIIGEAETA
                     LWALHTALSAGQSLDGVPSLLAPKHGVVALRSRGRESLRALPGPDFSLVDGTLYPYPE
                     PLVDVRMSRGCPWNRCTFCAITMHQEGYRALPVAEVSQALIAAHKQLGASFFRFRDDL
                     LTPKQLLQLSLVASQLPFAIRFSVRSRFEPDLTADVLAAAHAAGLEEIWLGLESAVPR
                     VRTLMKKGVDQPVVERILKDASQIGIRVRALCLLGYPGETADETRATIDFLDEHQHEL
                     ASCALTPFLLMRRSPIGADPQSFGVTVTPDVRPRHERLRPSLAATWPGALGRAFLESL
                     LDEASRRLGPHFLTMTSGPSLAHAMIHASVLRRGWPKSARMLSVVP"
     gene            16874..17704
                     /locus_tag="PKL17_02730"
     CDS             16874..17704
                     /locus_tag="PKL17_02730"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNK43669.1"
                     /translation="MLEQELASAIAAVQRAGQVLLEVYATPFEVSWKGKSDPVTVADE
                     RANALIVAALREQFPSDGIIAEESPDHLSAISKRRIWYVDPLDGTKEFIAKNGEFSVM
                     VGLAIDGEARLGVVYQPTEDKLYTGIVGQGAWLVERGQKTPLQVSEKSDPWTLGLVVS
                     RSHRPKSTETLTRTLGITKESVSGSVGLKVGLIAQQQADLYVHVSDKASAWDACGPEA
                     VLRAAGGRFTDLLGQPFVYGTTELRTTRGILACNQAAFASLLPTVTEIGFSDGLLQRT
                     "
     gene            17738..18217
                     /locus_tag="PKL17_02735"
     CDS             17738..18217
                     /locus_tag="PKL17_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43670.1"
                     /translation="MADERELGGPLDPLPDWQLFASVATQSELLDEVAQKTSGIGSVA
                     EAGFDAAAVASVSVPDPTLDGMASSANPAGPPVGLAFATTYAPVVAKPIQPAMTTQQA
                     MTVQFVDLLRPLVRSVFLRPLPTLADVAPDVEPQVRQIAPTGSDYRPGSIGKAIHGA"
     gene            complement(18237..18827)
                     /locus_tag="PKL17_02740"
     CDS             complement(18237..18827)
                     /locus_tag="PKL17_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNK43671.1"
                     /translation="MEKPFAPSCERNRDPILAVLQKHFADRRDVLEIGSGTGQHAVYF
                     ARALPQLRWQTSECAEHLAGISAWLDEAQLPNTPPPIELDVTGTWPRQTFDAVFSANT
                     LHIMPWTAVCQLFAKLHNVLTHDAKLVVYGAFNYGGRFTSPSNAAFDDWLKASSPQKG
                     IRDVESVHALAATAGLALVEDCEMPSHNRCLIWQRR"
     gene            complement(18840..19973)
                     /locus_tag="PKL17_02745"
     CDS             complement(18840..19973)
                     /locus_tag="PKL17_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK43672.1"
                     /translation="MRRTWGQTLCVGLGLSVSGTVLAAPPAKKPMPTPAVVDPAAAAQ
                     KTMRAQLSLGRTLAGKGKHSEAIAAFESALQAVPDEPRVLSELGVTLRASGNLPRAEE
                     VCKKAVDAAKEPPLRASALYNLARVLSQKGDKPGAVVALRASLKLRENRIVRETLLDL
                     DPTAQSEALSPEPLEGPLLSVDAWCKKQSGPCHLGSDAAVQGKLDRPTGPWLEAQVFS
                     AGDSPEDCVLAVRTARGFYFSRLFSCRESGYRQTERATIETREILSPPGAKLLVTLDG
                     VDSMRDFDEARGQSFCCVEAKTKDLVVCGVGPSGTPHCTRSIGLHPGLDAQKRAGTVQ
                     LVPNYGGQELVLERSDGKPIEDGKAPLAPNLKALAGRHRVVFP"
     gene            complement(20023..20979)
                     /locus_tag="PKL17_02750"
     CDS             complement(20023..20979)
                     /locus_tag="PKL17_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNK43673.1"
                     /translation="MDKRKALGRGLGALIPNLPAAVHQTPVGVDDPSGSASAGGVRPQ
                     VARRDFFFCPIEEILPAVDNPRQRFDEQRLSELADSIRAQGLVQPLVVRMRTPEDKPG
                     DGSFILIAGERRWRASQRAGLKDVPVVVKDVSAAQAFELALVENLQREDLNPIEEAEA
                     YRRLSDEFGYTQEELSRRVGRERATVANALRLLKLPGKVRDQVATGQLAMGHARALLG
                     LEDSRLIEENAEQVIKKALSVRQTEDLVRRAQKPSKDKPGKDKPQPSSSVRDVEQRLQ
                     RALGARVQLRQKTPQSGIIEVHYHSLDQLDGLLLKLTGEESD"
     gene            complement(20983..21795)
                     /locus_tag="PKL17_02755"
     CDS             complement(20983..21795)
                     /locus_tag="PKL17_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43674.1"
                     /translation="MGRVIAVANQKGGVGKTTTTVNLAAALAEEGQKVLVVDFDPQGN
                     ATSGLGFVRGSVEQGIYHALIDRVSLRELTQLTVLDTLWLVPSTADLAGAEVELVSAE
                     RREWHLHDALSEVRGRYDYILVDCPPSLGLLTLNALVAADGVLVPMQCEYFALEGLSY
                     LLSTLDKVRGLWNPGLELEGILLTMVDTRTSLMQQVESEVRGHFGPKVYRTLVPRNIR
                     LSEAPSHGLPIMKYDPASRGSYAYGQLAKEFLEQRRAAANMPTETTAAQGAR"
     gene            21922..22140
                     /locus_tag="PKL17_02760"
     CDS             21922..22140
                     /locus_tag="PKL17_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43675.1"
                     /translation="MRLKSLSARPSSNPRQIVLAADPELDAMLDALFASELCPTWQTA
                     EQAPAAEGESNAVESTTAACSPGIVRAA"
     gene            complement(22097..23716)
                     /locus_tag="PKL17_02765"
     CDS             complement(22097..23716)
                     /locus_tag="PKL17_02765"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNK43676.1"
                     /translation="MLGLMQDRPLLISSLIEYAASVHGDTDVVSRHADGVVRSNWREV
                     HARAKRLAKALTARGVKPGDRIATLAWNSHRHLELYYAVSGIGAVLHTVNPRLFPEQL
                     AYILNHAEDTHLFFDAAFMPLIEKLRAHLPTVKEYVLLGDEAATVGHPGVISYESFLH
                     GHDDSYRWPVFDENTAASLCYTSGTTGNPKGVLYSHRSTVLHALASCMSDVLGLSSRE
                     SILLIVPMFHACGWGVPYSAALVGAKLVFPGAHLDGASVCELLQSEECTLSLGVPTVW
                     LGMLGYLDKHPDKKPKHLRRMLIGGSAAARSLIARLEGELGVDVMHLWGMTELSPVGT
                     ASLRPRRWDKMSVDEKLDLKVKQGRIMWGVELKIVDDNGQELPHDGKAFGMVKVRGPW
                     IAKEYFRQEGGPIVDEDGFFSTGDVATLDGEGFMQITDRAKDVIKSGGEWISSIDLEN
                     AALAHPAVQEAAVIGIAHPAWVERPLLLAVLKPGQTTTRDELMSVLAQKVAKWWLPDD
                     IQFVSELPHTATGKLNKLRLREQYRDYKLPS"
     gene            complement(23762..27169)
                     /locus_tag="PKL17_02770"
     CDS             complement(23762..27169)
                     /locus_tag="PKL17_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNK43677.1"
                     /translation="MSVDNPNPSPSAVKTQARWPKILGLAVGGTLLLFFVVVLIVGFA
                     VDLGQVVRKQIVAQLPEVQKKIGREVSVGTVSLRLLPKLRLRVRDVKVSGQPGQTGIL
                     GEPLAEIGSIEADVSIVPAILTMGRRINVSRVEIANGKVQVARLSGGRLSYEDVLDHL
                     ATQPKDDTPLTQAQIEKLAGITIERAELTQSAVLFHDLSTGKAAPPLKIDGIHLQITD
                     GKLFGETQLSLDMALLQLVPNFHLGMVVGPLPKDLRPDPAWTVLRKLELKLQSFQIEP
                     LLAFLPASTGTTIERAMVEADLLIKSPPDSGKLSVAGKAGLRGLQLRQNGVPAKLYSL
                     SKGAPTDVMLSMNVDLAMLAGDIRVEKARLDIADMNVTAEADLRNLWKSPAVHSLQVA
                     SHGLKLERLLALLPKTAVPTGTELSGPLSVSGSASGTPTAAAVKVSLDMTESNIATPQ
                     LRKPTGMPLTLGLLGTVKDPGFRVDRFGLVLGPLSLLLHGELKSGNDFDLTLDSGTVD
                     LDKLLRLLPMVADGSAASGKKKKKGPTIDGDLRVSGGLTKKGDQFSAHAKVKMQNADF
                     EQDGMDLSGTAELSAEVKQSPSNSSVDANLDLTPAALHVSGSVDKDRGVPMRISLSAQ
                     KTPTAVQIKQAQLELPGGLIKLVGSADLAKKYLDMKVPLVDLDLAKLAQVVPQLRQGA
                     AGGLLDSKLTFGLSLSGNPEQLSSVRAKLDQFKMQVAGGTILGGASVQGLDEPKQASF
                     DFSADYLDLDKILGEKKGGDDDEDAAPSKEVQVPKVLKHLEAEGKIKVASGKLKGQTM
                     RDFVLEMTLSGGKLILKVMRMQAVGGSILLSGTSFDFAPSKPKFAIKAKLDKVDLHEI
                     ISFKSNDLAKKLQGHGSMDLSADGQGLRWEDIAPRLLGQLDLGFTEGKLVTAKLGSQV
                     VGPLMQRLGLSGQSGDEREMSMKNLTAQFKISDGRLRTNNPIRYQTEQGAIQLTGSIG
                     LDKTLDLLGDLQLQPAVISALTAGKVVPAAPVPVALKIGGSLSQPQFQLADPVKTAAA
                     LAAAIAKGRGQDLLNRAVGGQAGGALGGALGNVLGGQKTGGQAGGLPNLPTNLPTNLP
                     TNLPTNLPTNRPAGQPGQPGQPNVQQQLQDAQKRLQQGGGLGGLFGR"
     gene            complement(27235..27909)
                     /locus_tag="PKL17_02775"
     CDS             complement(27235..27909)
                     /locus_tag="PKL17_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNK43678.1"
                     /translation="MVSASTVVVMHQYVTPKLPVATVEVPQVSGLTVEQARAITEPLG
                     LLLVLDGEKIPDADKVQTGSLFEQRPLKGSRVAHGGEVHAFIAIPPQLSTVPALVGQV
                     VAAAEQQLQVAGLRLGTVSEELSATIPPGQVISTRPAAGEKLRKNETVSLVVSKAPEQ
                     FDLPSFRGRSLGSAKVQLEQLGLVLGDVRKATDDNAADGAVLRQNPPAGSKVGKGQKV
                     DLVVND"
     gene            complement(28046..29299)
                     /locus_tag="PKL17_02780"
     CDS             complement(28046..29299)
                     /locus_tag="PKL17_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43679.1"
                     /translation="MRRYQLKRAGVSAALCVSALTMAMSSAWAQGAAQTGAPDPASVP
                     PTTGMTQIPPPPPYPLVPGGDAAAVATPVPAQVAAAPVPTPPPPAQPSTPPPAPPPYS
                     LPFGLRPIVPVTVLRLDTVFAFYQQNIAAAGQPERYENGFAGVFLPTIGYRFTPSWMG
                     IVRFGMVGNRPPRITGDETGADNCEDTTTTDPTTMQPKTVLTKCGVATSNLFLGGFYS
                     HKFADNFRFSLFFGTTIPVGSGTSAATEPGASLAEAPTAQLARSAMDNVMFAVNDWAM
                     VEGLDLAYIGHRLTVQAEVTFFELFRVRNEAANPDVYKVNFTTGLHIGYFVANWMSLG
                     AELRYQRWLSDPKSVVADEANADKGLPVQGLRQNLSLGIGPRFHIKLPGGKWIRPGIG
                     YEPGLYGVMGNRKYHVLQIDIPILF"
     gene            complement(29310..30059)
                     /locus_tag="PKL17_02785"
     CDS             complement(29310..30059)
                     /locus_tag="PKL17_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNK43680.1"
                     /translation="MTEYSANVDRDALLRAEGYRLLAAAFRYPDQTELRALFSEELEV
                     ESALKPLAEALIPSVDDQLAGEYNRLFAQSVAVSPHERAYTGGDLGPGLGQLSSLYEA
                     FGLRCGGRDTEVPDHIGVELEFAAFLCLKEALHQEEETPEAQEAVSIVRAARKTFMQE
                     HLGRWVSAFASKLQSAAHHPYYAELAHLLREWVQKDFAVQGWEMPVGGRHLPVVAPDG
                     QTEPLDDEPMTCPSALQEEESVPLSTLLPSG"
     gene            complement(30056..30946)
                     /locus_tag="PKL17_02790"
     CDS             complement(30056..30946)
                     /locus_tag="PKL17_02790"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNK43681.1"
                     /translation="MKTTFLFPALFLLPVMTGCPSGDHETGAPSKLTGEIRVTSVGEI
                     PLSDPLSPAWNGTPETAVSMLPQTVAYPNLVKQSVKELRVRTLADARFVAVRLEWDDP
                     TLDEKLEVDKFTDGVAVEIPLGDADKTNPMMGDVQNPVYICHWKAAWQRDVDRGRADV
                     QDYHPGYVSDRPPFVSGGYPYPVDEAFQTDNARRYLPGTAAGNPVSKIFRKWPVEELQ
                     AEGFGSLADHTYQDAQGKGVWKDGKWTVVLAVPRQSPDRANPMLVSGKAMMGFAVWDG
                     SNQNVGGRKHWAPFVKVVLP"
     gene            complement(30962..32089)
                     /locus_tag="PKL17_02795"
     CDS             complement(30962..32089)
                     /locus_tag="PKL17_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015320289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNK43682.1"
                     /translation="MSKVQNIHLGREMEYPYEGSRPNKQIAYIFDTNKCIECQTCTVA
                     CKTTWTAGKGQEHIFYNNVETKPYGFFPHGWDVKLLEMLGPGEWQDGKYNGKTIFEAA
                     PPGERVLGFLPEEEDYTSPNLGEDVCSGDVQRGAFFQNIHNTWMFYLARICNHCTYPG
                     CLSACPRKAIYKRPEDGIVLVDQSRCRGYRECVKACPYKKIFYNMVTRVSEKCIGCFP
                     LIEQGEQPQCVKTCIGKIRLQGFLSKPGEEKADNPIDYLVRIKKIALPLYPQFGTEPN
                     VYYIPPLHVPRLFLQQMFGQGVEEALKTYRSAPADKQLLAALLMFGNTPKIIHKFKAD
                     KDHAVGFDGMSQEAVRVPFTEPFFAREMFDKKHGVVRLNIT"
     gene            complement(32100..35573)
                     /locus_tag="PKL17_02800"
     CDS             complement(32100..35573)
                     /locus_tag="PKL17_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK43683.1"
                     /translation="MSSSTFSRRSFLAALGGTAGVAAATASKKGWAFNFLEPVSVDNP
                     LKSYPNRGWERIYRDLYAYDSTFTFTCAPNDTHNCILRAYVRSGTIVRIGPTFGYGEA
                     VDLTGMKSSHRWDPRCCQKGLALVRRFYGDRRCRYPLVRKGWLQWQKDGFPRDPATGK
                     VDPKYLQRGKEPFVRVSWDEAFDLVAKGMHNVAASYSGEQGKKWLLAQGYDPHMVEAT
                     HEVGTQVIKMRGGMPLLGTTRIFGQYRFAQTLALLDAFVRKVGPDKALGARGWDNYTW
                     HTDLPPGHPMVTGQQTVDVDLSMAEHAKLIIVWGMNWITTKMPDAHWLTEARLKGTKV
                     VVIAAEYSATMNKSDMGLVVRPGVTPALALGFAHVILKENLYDAEYVRSYTDLPSLIR
                     LDTLERLNAKDVAGIKLTGALGAQILKKGEKPAPDFQQKDAIIPEDMLAKFDPFVVWN
                     EKTGKAECISRDEVGKKFKKRGLVAALEGEFEVTLTDGKTVKCQPVFQLLKQYVMDNF
                     APETVESLTWAPKDGIVTIAREIAANKEKTFFACGMGPNQFFNNDLKDRTIFYLASLT
                     RNIGFVGGNIGSYAGNYRTAYFNGLPQYSLENPFDVELDESKPSRPKSFLKFESVHYW
                     NHSDHILHMGKEVLTGKTHIPTPSKAIWVSNSNSLIGNAKGHFETVVNVLPKSDLVVV
                     NEWWWTASCEYADVVFPVDSWAEFKHPDATASVTNPFISVFPRTPIDRIYGTRSDIEC
                     SAGVAASLGKLTGDKRFHDMWHFVYEGRVDVYLQRVWNMSNMAHGLNFLECEEQAKKG
                     IPTIILSRTYPKIVGWEQAYEDKPWYTRTGRIELYRDEKEFRESGENLPVHREPIDST
                     FVEPNVIVGKPHPAIKPKTPDQYGVPLDQLDGDTRQARHVVKTWDELRNTKHPLQQKD
                     PAFRYLFHTPKYRHGAHTTPVDTDIIAVWFGPFGDMLRHDPRTPYVTEGYVEVHPDDA
                     RELGCEDGDYVWIDADPADRPFRNPEKATAEDKKLGRLLVRLRYYPGTPRGVTRMWHN
                     MYGATYGSIKGHESNQNGLARNPETGYQALFRYGGHQSCTRSFIKPTMMTDTMVRKNS
                     FTQQVGQGFEPDVYCPTGAPRESFVKITRAEAGGMGGKGLWRPAQKGLTPGHETEAMK
                     RFLKGEFISSK"
     gene            complement(35892..36284)
                     /locus_tag="PKL17_02805"
     CDS             complement(35892..36284)
                     /locus_tag="PKL17_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNK43684.1"
                     /translation="MTRKLVFASLALLSLALAPACNKGGASGSAPAISPQAKQEAQEI
                     FASRCAVCHGAQGAGDGAGSAGLTPKPRNFQDKAWQGSVKDDYIVSIIKSGGAAVGKS
                     PAMPPNPDLAEKTEVLNALAAHVRGLAK"
     gene            complement(36289..36732)
                     /locus_tag="PKL17_02810"
     CDS             complement(36289..36732)
                     /locus_tag="PKL17_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNK43685.1"
                     /translation="MLSQTVGYAIAALGHLSRFQKQPGPQFVRDIAKATGIPPAYLAK
                     IVHTLARKGYLTTQRGTSGGVSLARAAEDVTLLEICEVFDDPIVQKRCMLGTTVCSDE
                     RNCPAHCFWTEERERVAEFLRRTTLREIARFEDDKAPLVKENLKR"
     gene            complement(36931..37003)
                     /locus_tag="PKL17_02815"
     tRNA            complement(36931..37003)
                     /locus_tag="PKL17_02815"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36968..36970),aa:His,seq:gtg)
     gene            complement(37071..38285)
                     /gene="rocD"
                     /locus_tag="PKL17_02820"
     CDS             complement(37071..38285)
                     /gene="rocD"
                     /locus_tag="PKL17_02820"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNK43686.1"
                     /translation="MTQMRRNLPEKTQHFVDLEERFGAHNYKPLSVVIEKASGAWVTD
                     VEGKRYLDCLGAYSAVNQGHSHPRIVAALVEQAQRVTLTSRAFRNDQLPLFEKELCEL
                     LGYERMLPMNSGAEAVETAIKLARRWGYQKKGIPDGQAEIIVCANNFHGRTTTIVSFS
                     SDPLYRDGFGPFTPGFVTVPLGDLAALQAAITPRTCGFLVEPIQAEAGILIPPDGYLA
                     AAAALCKQHNVLLIADEIQTGLGRTGQLLACQHENVRPDVVILGKALSGGVYPVSAVL
                     SSADAMDVFRPGSHGSTFGGNPLACAVARAAIRVLDDEKLCQRAATLGAPLLSRLRQI
                     KSPYIREIRGRGLLVGIELDRPARPFCEALAERGVLCKETHDFVLRIAPPLVVSAEDL
                     DWAVDQFAAVLG"
     gene            complement(38293..39270)
                     /locus_tag="PKL17_02825"
     CDS             complement(38293..39270)
                     /locus_tag="PKL17_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43687.1"
                     /translation="MLVRVLYHDCCFDGATSAALFSEFYRRCVLPDAQFEYVGMNHGP
                     GDVFAGAFIGGPDTEHVCVDFRYSTDSRLSWWFDHHQSAFLSDQDQAHFHAAQNRRHF
                     YDPKAQSCSKFLATCCSSAFGFDPTPFADLIAWADLIDGARFVSPEQAVMCREPALQL
                     MTWVEQNRDPQLKLRFISALRSQSLDEIAAESYVQDGIAPVVKMRDEVLRVLHERAVV
                     LDGVVTYDVTDVPQILVSKFAPYYLFPDAHYVVGLSSLPGRTKISVGSNPWRAERRTA
                     NIANLCGRYGGGGHPAVGAVSLKTDDVALAQKYAAEIAAVLVAAARTEI"
     gene            complement(39307..40743)
                     /locus_tag="PKL17_02830"
     CDS             complement(39307..40743)
                     /locus_tag="PKL17_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNK43688.1"
                     /translation="MSFFDPILTRRILGLSAPVMLAMLTQTLINQVDHVLVGHLPQAE
                     STPGQTALQISVILLWAFGGMLAAISVGTQALAARRIGAGDIEGAGAVSTNSQLLAVV
                     ASIIVTAFTYAMAPKLFRLMNKDPAVIALGVPFLRWRFLQITSMVWTASLKSFFDGLG
                     KTRVHMGVAIVMNIANFLMCIAFIYGPDQPGIPVVDGIHSLLLSMFGDKLVHMGVPGA
                     GLASMLSSYVGLFIMLGWSLRGEYRKFHIRRVTNASASTIWQIAKLSVPSGIATMFAM
                     GGFGFVLYVVSRLDHMAGHGVGRTIFSTATSNIINVLQIVFISCLAYGTATATLVSQN
                     MGATEYDLAERFAYQAARIGAVLFLVLGLILAAWAEPILHFWNPDPDVIAVGAPILRV
                     LGFFCPLISVALVFTQALYGAGNTMFVMVAEGILHFSCLIPMSWLAGITFGLGMWGVW
                     GSLIFYIAMLAVVMFIEFRRGHWKHIRI"
     gene            complement(40896..41795)
                     /locus_tag="PKL17_02835"
     CDS             complement(40896..41795)
                     /locus_tag="PKL17_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNK43689.1"
                     /translation="MPVEAVHQSALVDSLSRASAWVRRATSGMNHDAARLGAMFVDLP
                     YFDRFAVAVVRYALRRPQAPSHFGELFHQKTPITLGRLFGEAGQRLAQKSATEEAGKT
                     LTALALGYVSHAAVDTSMHPHINALARRRAARSGLCPSQEHHEIEKFHSLIFHEQRFS
                     ADLMGTEKLQRYIEVDGRILGDDGAIGDAVQAALCTVHGQGPTRADLRRYAAGYLQYC
                     ALIGNAIVGPRIGPRKQREAARAEVYDDFDFPGRFASAVEKSLSYLDAFGAYLSDGVF
                     DESAKRALFAQVPEGSIDPDPTT"
     gene            41896..42699
                     /locus_tag="PKL17_02840"
     CDS             41896..42699
                     /locus_tag="PKL17_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNK43690.1"
                     /translation="MKAILIAAGMGRRLSPYTDDRPKCLVEIAGKPMILHQIANLRRV
                     GVSDFVVIRGYLRDKLTDVLSREPGVRFIDNFDYPNNNILLSLMCAKDELSSGFFCSY
                     GDIVYRPEVSEKLAQSRADIGLVVDPYFAKVYEGRTEHPIGEAELAEVIDGKVRRVGK
                     KVVPTAEAAGEFIGLWRASAQAGVALRTLFEKRLAELGLSSPYGRAQRLQVAYLTDLF
                     NDLITEEARIGISVDGVMIDDPQSWREIDTVQDLERARAVVSWQSAAGG"
     gene            42701..43912
                     /locus_tag="PKL17_02845"
     CDS             42701..43912
                     /locus_tag="PKL17_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43691.1"
                     /translation="MKILIADAFSEAHLSAFSRLGLDVDYRPQLSAAELPTAIAGVGI
                     LVVRSKQVSAATIDAGSALALIVRAGAGVNTIDLDAASRRGVFVANCPGQNAIAVAEL
                     TMGLLLALDRRLPDQVAELRAGKWNKKEFGKAAGLHGRTLGVIGTGSIGHAVIHRAQA
                     FGMKVMAWSRSLTAERARELGVERAETIPALCAASDAVTVHVAYVPATRGLVGEAALQ
                     RMRKGAMFINTSRTEVVDTTALRKAIGEKGLRVALDVFDHEPEKAEAPFADDIVQLKG
                     LYGTHHVGASTEQAQSAIADETVRIVDAFVRDGTVHNCVNILPPDETPARAELVVRHY
                     DKVGVLAELLGAVRRHNINVETMQNTIFLGAKAACARIRLAVRPSSELVSEIRQHPDI
                     IHVDVIELPET"
     gene            43909..45153
                     /locus_tag="PKL17_02850"
     CDS             43909..45153
                     /locus_tag="PKL17_02850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNK43692.1"
                     /translation="MIQIDSLSSASRLPGLAHVPIVPRAVQRLRAGIPLVLRSDVAAE
                     PSPDSEEVLLCDLRGQPIATALWVAQGPLAARLWSLSGESFSEETIAERLKAALVRRR
                     PILQGGERDAFRLVHGEADLLPGLFVDCYADAAVIQTATRAMDRHKSEIADLLLQLLP
                     IVRVLFRDDGSARDMESLPREKGFLRGGPLRMARFHDAGSTMEADLLADRKTGSFLDQ
                     QENHLEVAQWAQSICPGGEALDAFTYHGGFALALARAGLGVIACDEDPQAIGRARRNA
                     ELSGVAVDFHVHNAFDLLRSYEAAGRRFDVVVVDPPALAKRGRSAGSTQHAMTQAMRA
                     YKELNLRALRILRPGGLLATCSCSGRVTVEDFAQMLRGAAQDAGRPLQLLWRRGAAID
                     HPVLWGLPESEYLKCWLFRVLA"
     gene            complement(45166..>45672)
                     /locus_tag="PKL17_02855"
     CDS             complement(45166..>45672)
                     /locus_tag="PKL17_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNK43693.1"
                     /translation="ASLILGTPIAVVSLVDEHRQWFKAIVGLDAKETSRDVAFCAHTI
                     HGSEVFEIADATKDVRFANNPLVTNDPSIRFYAGAPLITSEGSEIGTLCVIDRQPRAL
                     SQNQRDILVRLSRAVTLLLERNRQHAARPSISGESVALPLLPDLSPSSLPLVTESQRL
                     QKRVMQKS"
BASE COUNT         8794 a        14118 c        13945 g         8815 t
ORIGIN      
        1 aaaagaccag caggatcgtc atcatgtcca tcatcggcat gatgttgagg aagttaatct
       61 cttccgcctc ttcctcgact ttgcggcgca gcttggaaat cgcgcgccgg gcctctcgac
      121 gatcaatctc cttgacggcc atcagttcac ccccgcgccg agaagcacac cgggaaacag
      181 cgccttggga tcagcgcccg cggtctgccg catcgcatcc atcgtcgata ccagcagctc
      241 gtaaggaatc tgtggcgtcg cggtgatgat ggcgcgctcc tcgtccggat aggtgttctt
      301 gagctgaaca aggatctggg ccaacgccgc catgtcatgc ttgccatctg gcccaagcgg
      361 caccgtcggc agctcgcttg tcgtctgctg cacgccttgc ggggtcagga caaagccccg
      421 gaacagcacc gccccactgg tcgccagcgt gtaacccttt tcggtgatcg agaccgtcag
      481 gttcagctcg ggtttcggtg gggcgctgtt ttgggtcgtc accaccgaag aagtcggcaa
      541 gttgatgttg atgctggcca gcgaggcttg gaacgtcacc gtcgccagca agaacatgat
      601 gatgttcatg acgatgtcga ggtatggcac aaggtttagc tcgccggatt cctccgtcag
      661 ctcttccccg tgctcgcgta gcttccgcag ccggaagcgc aacctctgga tggtcatgga
      721 agtttgtccg ctcgctagtc gttgtcttct tcgtcttcga catcacgcgc gcgccggttc
      781 ttttcggcat cgcgacggct gccaccccga ccgccatcgc ggctttcttt ttccgcggcg
      841 ggggctggtg gattggcaat caggtcgaac agaagatttt ccagacggag cgcaaagctc
      901 tccagttcct gctgtttgct cttgctggct gccgacagga acaggtgacc aatcatgcag
      961 cttgcggcaa tcgccagacc cagccaggtg ttgttcatgg cttcggcgat gcgggtcgcg
     1021 agcagcgagc tgcgctgatc tgccgccgcc ttgccgatgg cggcaaaacc gccgatcaga
     1081 ccgttgatgg tgccaagaag tccgagcagc gtcgcaatgt tcgcgagcga ccacaggaca
     1141 ccaatgcgct tcttcagctc gggtgtggca tcggtcaggg cttcttcgat cgctgtcgaa
     1201 acagcggctt caccgttttt gagcttcgag agcccggcct tggcaacgag cgcaatcggc
     1261 gcatctgacg actgcgccag ccgctgcgct ttttctagct gtcccgccga aagcagcttg
     1321 cgaagctgtt ccaaaaacgc ccgcgcgttc accgcacctt tgcccagaaa aaacatggca
     1381 cggtcgacga taagcgtcac aaccacgacc gagcacagca ggttcacgta catgaagaac
     1441 ccgccctgct cgaagaactc tttgagctgc tgcatgagaa attgtcccta cggaagcgac
     1501 tactgcatct ctagcatgtc gccgaggaaa gcgaaaattt ggtcgtagga tccggtatcc
     1561 acttcctgaa agtcaggttt accgggaagc acttcgtccg caatcacatc ggcaatgcgt
     1621 tcggaaacat acgagcggac cgactggtct ttggccacat cgatcacaaa cagcaccttg
     1681 gctttgacca cctggctgtc gaagtcgcgg ttgagcgtaa acagcaccgc cttgccaccc
     1741 ttgccatttt cgtagccccg ataggccgca tagtacgtcg tcactacaga agtggtgtcg
     1801 cgggtatcaa gcttgtaaaa cacgctgggc ggaatcaact cgctcatgaa cgctccttgg
     1861 tacgccgcca ccatggttcg gcgggccggt cctattatgc cggtcgccgc ttcgtctaaa
     1921 aacccctaaa tttcactggc cgcaccccaa accgatcgac gaagcggccc aggaagcgcc
     1981 caccgcacgc tcagctctcg tatgatgtca ccgagcggca gcatccaagc agccaacgga
     2041 aaggcgagac ccgatgccga ttgtgcgcac cattgtgttt gtttcaatca tgacggcggc
     2101 catggtgggg ctgcactact acgtttacct acgggtgacg cgcggcctgg cactgggacc
     2161 gtcagaacag cgcatcctca aagcggtgct gaccgcgctg tttgcgcttt tgtggctcag
     2221 ctttccgctg acacggcttg tgtcgcgaag tgcggcgctg ccgtttatgt gggtcggcta
     2281 catctggatg ggcacgatcg tgctgtcatc ggtggtcttt ggtctgtttg atctgtcgag
     2341 gtttgtcgcg ggactgcttt cgcccgcggt tcccatcgat gaatcgcggc gggccttgat
     2401 tgggcgactt ttcgacgtcg cggcacttgg cggggcgacg cttgtgtcag gctgtgcggt
     2461 cgcgctgggg ctgcgcccag tcgtcgtcaa gcgaatcgag gtcgcgctca agaaactgcc
     2521 agcagagctt tcgggctttc agattgtcca gttgaccgac gtacacatcg gaccgacgct
     2581 ggacggcaag tggctcaccc aggttgtcga gcaggtcaac gcacaaaacc cggatgccat
     2641 cgtgatcact ggggatctgg tcgatggcag cgtcgcgcag ctcgggcagc acgtcgcccc
     2701 gcttgccaac ctgcgcgcca ggcacggggt tttcttcgtc actggcaacc acgaatacta
     2761 ttcaggggtc gacagctggc tggccgagct ttctcgactg gggattcgcg tgctgcgcaa
     2821 cgaacacatc acgctgagac cgagcaagct ggcgggcgaa ggcgcaagcg atctggggat
     2881 tgatctcgtt ggcgtcgatg acttccatgc cgatgtgttt ccgggtcacg gccccgatct
     2941 gccacgcgcc gtaaaaggcc gcgatcccag ccgtccggcg gtacttttgg cgcaccagcc
     3001 agccgccgtc atggaggccg cgcagcacgg catcgacctt cagttatcgg gtcacaccca
     3061 cgcgggccag atgtggccgt ggggttattt tgtgcggctc cagcagccct acgtctatgg
     3121 actgcacaaa catggcgaca cccagattta cgtgagcgcc ggcaccggct actggggccc
     3181 accaatgcgg ctcgcgtcgg aagccgagat cacctgcgtc atcctgtccc gcgcggggta
     3241 aactgcgaca agcgggaacc gcggcggcgc tttcgtcgtc tagccgggaa gaagggaaac
     3301 cacgcgccat gacacagacg aagcttttgg ctctgtccgc acttttggca agccttttgg
     3361 caagcgctgc gcacggcgat gaaccggcag tcaaagccga cgcagcgatc aaggcggaag
     3421 ccccagccac cgaccgggcg gcgcgacggg ccgagctggt caaagaagtg gccgcagcca
     3481 gggccgaacg tcggcaagag ctgcgcacag gcgaacgacc cgccgcaccg gcgagcggaa
     3541 gcgaacggat cgccgaagcg caaaagcgca gagaagccca gagcacccgt cgcgacgaag
     3601 gccggatgtc cgtccgcgag ctgcagcgcg aacaccgaga agagctacgc gaacaccgcc
     3661 gcggcatgat ccgcagaacc ctgtaaccgc ggcccagaaa gccgcgagac cctaaaacaa
     3721 gcgaagctgg ggtttttggg gtttgggttt tttcggggct tcgctttcgg tcagctcggc
     3781 ggggacttgc gacaaaaacg gacagggcgc gcagccgtcc gcttcgattc gcgacagata
     3841 caagtgatct ttggcgcggg tcatgccgac gaaaaacagt cggcgttctt cttcgcgctg
     3901 gtcgtcgtcc cggcctggac cgggaatcac agacgcttcg agcccgacga caaacaccac
     3961 gggaaactcc agtcctttgc tgccatgcaa ggtcagcacg gcgattcgct ggtcttccag
     4021 gtggtcgcac tcccagatgg gatttggcgg cgggcttgca tcgggcagcg cggacagagg
     4081 ggtctgtgct ttgctgggac gcgaaaacgg caggcccgcg gtgtgtagcg cttcggcgac
     4141 ggcatcggcc cgtgcgatgg tgcgggtcaa aattgcgatg tcggaaaagc catagctgcc
     4201 ggcccgccac aagtccgtca ggcggcggtc gtgcagggac aggctgctgc cgccgatgag
     4261 ccgttcgatt tcgtgcacaa tgcgcaccgc ttcgctgcgc aaagagggac tgcgcagaag
     4321 ctggatcttg ccgccggcgg gccgacaggg cacaggcgga ggggcggtgc gaccagggtt
     4381 tgcgacaatc accgcactgg cggcgccgac aatttcagcg gtgctgcggt agttttcgca
     4441 gaggtagcgc acctgcgcgg ctggaaaatc cgtcacaaag tcgcggaaat ggcgcacttc
     4501 gccgccgcga aaggcataaa tcgcttggtc ggggtcaccg attgccagca ccgtggcggt
     4561 cttgcccagc tcgacggtca gggcacgctg gaccgcgctg acatcttggt attcatcgac
     4621 ggcgacaaaa cgaaacaacc gcgacgtaag gccagaccgc agcgaagcca gggcccgcag
     4681 caacaggtcg tccaggtcca gcacgttgtg ctgggcacac agcgcggcgt agcgctggaa
     4741 gaccacgagt tcgtcggggg aaacgtcgag atccccacgg gcgaggcggt ctggatagtc
     4801 gggatgctga cctttgcgca gcgacagaag cgacagcagg ctggcggggc ttttgcggca
     4861 gccactgccg acccgcgcct cttcgacaaa ctggagccgc tcgctttctt cggccagccg
     4921 cagcggcgaa acgaggggca ccagcgccag cgagaagtga tgaaatgtcc caatcaggag
     4981 cgcgtcggcc aaaggaccga gcaggccgga aagtcgctcg cgcagctcac ccgccgcccg
     5041 tcgcgtaaat gtcacggcca ggatctcttt gggccggcac agatcctgcg caagcagctc
     5101 ggaaatccag gcgcacagcg tcttggtctt gcccgaaccg gggccggcgg tgacaagctg
     5161 tggccccgtc gcataggtcg caacttcctt ctgggccggc gaaagaccgt caagccaccg
     5221 agcgtttggc tggttcatcc aaacaggccc atctgtcggg atgtcgactt ttggctgcgc
     5281 tcgctcgggt ccagcaggcc aatccgcccg tattcgccat caaatcctgc ggcaatgcgc
     5341 agttcgcccc gtcgcatgcg caggatcgct tcggccaaga gcgaatcgcc agcacgggca
     5401 agctcggcgg gcggagccgt gcgcaggacc gaaagctcgg ggccaacctg gtcgagcagc
     5461 ttgaagtaga gccgctgcac cgcatcggaa gcagcggtct tcccaagcac atcggcaatc
     5521 acttcggtaa gtggaaccac gctgtcaaaa ggccgcgccc gtggcggacg ataggtgttg
     5581 tcttccggac ggtcagccaa agccagcacg cgcgacagca cgccgaccgt caccggcttg
     5641 ccacagcgcg gacacagacc ggcaagcgcc gctcgctcgc ccggttcgag ccgaaccccg
     5701 cagtcgcgat gcccgtcgag gtgatacttg ccttcttcgg ggaaaaactc gatggtgccc
     5761 agaaattcgt ccggcgagca gacttccagc gcacgccgca catcgacaaa cgtcggcgga
     5821 cttggaatat ctagccgggt cagctcgcgc ccgagcttct gcggcgagtg cgcgtccgag
     5881 ctggacacaa gtgagaggga gtcgagcgca gacagccgcc agttcatcgc cgggtcagat
     5941 gacagcccgg tctccagcgc aaagatgtgc ggagtcaggt cgccaaagca gtcggacaga
     6001 cggtcaaagc ccgactgcga gccaaagacc gaaaagtgcg gcgtccagat gtgcgccgga
     6061 accagatagg cagcggcatc ggtggacaaa agcagttcca gaaagtcgcg acacggcatc
     6121 cccaggatcg gcctgccgtc ggaatgcagg ttgtacccaa gacgcagaag ccgatcggcc
     6181 acttgatcgg cgacatcaag gctgggcagg acgatcaggt tgtgaacttt gcggacggcg
     6241 ccgtcttttt tgaaaatgtg cgaaatttcg cccgacaaga tgaagcgcac cttgcggcgg
     6301 cacgagcgcg gaatcgcctg gtcgagcggt tcacgaagcc ctgggtccag ttcataaaga
     6361 ccgtggccca ggccgtcgtc cgcggggacc agttgggccc gcatctcggc tcgccacgct
     6421 ggatggagga catcgccagt ggccacgatg tcgatgcctt tgcgctgggc ccactgatac
     6481 atgtggccaa ggtccatgtc ggcgctcacc gcccgagaaa aacgcgagtg aacgtgcagg
     6541 tcgagaagca gctccatggc agcagaccgt atcgaggacg acaccgcgcc gacaacacgt
     6601 aagggaaggt aaagcgacgg cgattttccg accgccagcc agcccaaatt accaagcgga
     6661 acaggcgctt ggcacccgca ggccgtggta gtctgtaacc atgccaaagt tccgcgccgg
     6721 ggcccccgac ctagtggtaa gccccgatgg caagtgggcc gcagagcgcg aaggcggcga
     6781 gctgcgggtg tacaacctga cgctgcccga cggtgcaccc aatcggcttg gtccttgcga
     6841 aacccagcga caagaagatc gacctggacg gattttcttt gtccaaaacg atcgactgat
     6901 gaacctgtcg gtcgatgacg gcggcgaatc gggaagcgcc gtggtggtgg ccgagcttct
     6961 gcagctgccg atgctggcca aaattgggcg gtcggtgcga attccgggca cgcagcgaat
     7021 cctcggcggc gggccaggcg gcgtggtggt tgcaccaacg ggggccggtg ccgagcttgt
     7081 ggtcccgcgg gaaagcgacc tggtggtcta caagctgttc gtgcgcggcg aggcgctgtc
     7141 ggcgggacat acgccggaca agcgcattct catcgagcag cgcagtggtt ttgaaatctg
     7201 ggacgcacag actcgacggg cgatggccaa gctggcgctg aacacccggc aggccgcgct
     7261 ccagcttggt tttttgggcg atggccggat ggtgtgggcg ctgacgtcgg cgatgccgat
     7321 gcggatcgag gtgttccggg cgtcggatgg gctgcgcttt tttgaactgg agcggccagg
     7381 acgagcgctg tgcgtcgaaa gtgcccaagg tcgcctggtg atcggcttcg aggatgacga
     7441 cacgatctcg ttcctcgact tcgatgtgac gacgcgagcg ctccggcaag tgcgattgcc
     7501 gccagactgc aagcgcccgc ttgcgtttgt ggtgacgccg agcagcaagc ttcctgagtt
     7561 gcttgtgcgg cttgatgaaa ccgaagaacc gctgttgcgg ctgccgctga ctcgcatcac
     7621 ggccaaagaa ccggcaccaa gaccggggac cgaaacccgc ggctcatcgc gcgagtcggg
     7681 cgccaccgct gggtccaaaa cgagcgcccg gcccgacggc cagactcgga caacaaaccg
     7741 ggcgatgcgc gacgccaggc ccgagtcgcg actgattcgc cggctgtcgg aggccgaatc
     7801 gcgggaggcc gaaggcaagt cgagcagcag cgcagccatc gatcctgtcc cggccacaag
     7861 tacaccgctt gcgtccgacg gtgagcccga cgaaaaccgg cttgtcgacc tggagggggg
     7921 cagcgatacc gacgagccgc aggcggtgcc actggtgccg gaaatttcgc tcgggggccc
     7981 aagtcagaag acgccgccgc cgctcgcgcc agtccaagcg acaccgccac cgctgcgggc
     8041 ctatgacgcc cagcgcagcc cggcggcatg gcagtgggag cttgcgcgct gggcacaaac
     8101 ctgtcagctc gtcgccgacg cgacaccgcc accatcatct ggacctctgc acgagctgtc
     8161 ggcccggctt cagcttcccc aagcggcgca acgggtgctc gggcttttgt atgcgacggc
     8221 gacgctgctt gggaccagac cgcatggcat gcggcccgtc gaactggcac aagcgctgcg
     8281 cggccagtgc gaagaacccg atgtcctggc cgaagtcctg ccgtcatcgc ttctgcgatc
     8341 cctggatctg ctggtccagc gccctgatgg acggctgcgg ctgcggcgcg aagtgagctt
     8401 gcgtctggcg ggtacgcccg atccagatgt ccagtggccg cagacggtgg cgcgcgaaat
     8461 gctgcaagcg ggcgtctatc tgctcgatgg accgtgcgtc cagaagccgg cgcgcttgct
     8521 tggacgggcg gtgctgcgac tggacggact tgtcgaacca acaccgcaca agcgcctgcc
     8581 tgcacttctg cgccgggcgc tgttgcaaga tgcagtgctg gtcgtagatg gactggcggg
     8641 actgtcctat ccagcgtttt cagccccgac ggtactggcg gaacttcagc cacagcttgt
     8701 gcagccgcgg gtgccggtgg tgatctgggc gatgccggaa gcggcggcgg cgctgggact
     8761 gcaaggccgc cgactgaccg aactttcgat tgtgcgcgaa ggaccggccc cgaacttccc
     8821 aagcagtgcg ctgccgcaag gcgtgctgtt ccgaccccca agtcccgcag ccaaggcagc
     8881 ggccagtccg cgcgcgattg ggcagcttgt catcgcccag gtgagcgaca ggcgcgccgc
     8941 actggtgctt ggacccagcg ccaacgccga agcctatgca cgggccgcct atctggccgc
     9001 ccgtgacggc gcgcttttgc tgctggaagc cgagcttacg ccgccgcgcg tggcgctgtt
     9061 ggcgatgctg ctaaaacaaa tcccggttgt cgtcgcggcc accccaccgg gcggacccga
     9121 caacccgtgg ccgcccgagt tacgacccta cgctttttag cgcttgccgg tgcgggcttc
     9181 gctggcgcga ccgcggcaag ttcccgagca gaagcgacgg acggcggtcc ggcccgagac
     9241 tttcaccacg aactccttgc cgcacttttc ccactcgcag atgcgcagct cttcgtcggg
     9301 accgagcttg cgcgcggcac cgtctttttt gccgcccttg gcctttttgg caggcgctgg
     9361 ctcggcttct tcttcggtct tgggcgcgct cttgctcgac ttggccgtcg ctttggctgg
     9421 ggcttctgac tcggccttgg ctgcggactt ggcgctggct ttgctggcag gcgcatccga
     9481 ttctgccgac ttggcaggcc ttgccgcggc ctcggtcttt ttggccttgg tatcggattc
     9541 ggccttctca ggcttcgcct cggcggctgg cttttctgcc ttggcggctt tgcctgcctt
     9601 ttcggctttg gctggctcgg cgggctcgct cttgcccgac tttttggcag gctcggcggc
     9661 tttggcagca ggggcggact tggccgactt ggccggcttg ctgtcggctt ccgcttcgac
     9721 cacgggcgcc gacttcgcag acttcggcgt cgcagtggat ttttcggact tgctcgactt
     9781 gctgctttcc accggctccg ctttggcgac cggcgaagcc ttcgatgact tcgacgactt
     9841 ggccgcccgg atcaccggct cctcaaccac ttccggcgca tcttccacca ccaccggcgc
     9901 ggcttttttg ctgctcgact tggtggatgc gggggccggc ggctccgact tgctcggctt
     9961 gcgggccggt gcggcggaat cagccttggc ttcggtcttg gcgctggcct ttttcgctgg
    10021 ctcggccacg gcagtttttt ccgccggagc gaccttggca gtcgacttcg gcgcggcttt
    10081 gtcagccgtc ttgtcagcgg ctttttcggc ggctttttcg acgggcttgg cagcgacctt
    10141 ttcggccttg gcgggcgact tgctcgacgg cgcggcggct tcttctttgg cggcactcac
    10201 cttaacaggc gctgctttgg ccgcttcggc tggcttcacc gcggctgcgg gatcggcctt
    10261 gcttggcgtc gtggcggacg attttttgtc ggccttggcc gactgggatg ccgcggcgac
    10321 cccggctggc agctcggcat tcggacgctc gggcggcagc ggtttttgac cactgtgggc
    10381 ctgatcacgc gaggaagtat cgacccgcac cgggcgaggc ttcgatggca atgggcgcgt
    10441 aacttcacat gacggaaccc gctccgccga gcgcggcacg atcggcgcaa aggggtccac
    10501 gggacggaag gccggcgtgg gaggggtaaa gtgtcgtccg ttgctcattc gtaagctccg
    10561 ctgtacctgc tgctagacag aaggccggac caaaagtgat cggcgccata ggttagcact
    10621 ttgaaactac cgctaggaca gaaaatcgac cttttcacca gcaggatttt cggggccccc
    10681 tttaaaagcc ttggcgcgaa aaaaagcaag ctttgcgatg ggttgcgggt gatcttgcgg
    10741 ttgcaaagtc agcaaggcgg gcgattccaa aaggaagctg aaagctttcc gccgacgtgc
    10801 tacgtagtgc gccgttccca tgagcggaga cgaagacagc gactgcattc gagtagaagg
    10861 cctgcgcaag cgctttgccg agtcgggtgg tgccgaaagc gaagtcttgc gcggcataag
    10921 ccttgcggtg cggcgcggcg agctggttgg tctgacgggg cgttcggggt cgggaaagtc
    10981 gacgctgctg cacatcatgg gcgggctcga ccgcgactac cagggcaagg tgtggctgct
    11041 cggggaagac ctggcgaagc tgtcggatcg agcgctcgcg agcttgcgca accgccacat
    11101 tggctttgtc tttcaggcgt tccacttgct gtcgcaccta agctgcctag acaacgtgct
    11161 tttgcccaat gcgtttgccg ttgatccgct gccgcgggcc gaagcggaaa agcgggccaa
    11221 ggcggcgctg gagagggtcg gtctggtcga aaaaatggcg agccgtccga cggagctatc
    11281 gggcggacaa aagcagcggg tggcgattgc acgggcgctt ttgtttcgtc cagaagtgct
    11341 gctgtgcgat gaaccgacgg gcaacctcga cgacatcacc ggccagcaga tcatttcgct
    11401 gtttggcgag ctaaaccgcg atgggctgac gatggtactg gtgacgcatg agccgcgggt
    11461 tgccgaagcg acgacgcgac tttgtcggct gcaagacggt ctggtgattt ccgacggaaa
    11521 cagcggcaca agcaaggagt ctttgccatg acgtttcggc aagcccagtc gctgctgttt
    11581 cattcgctgc ggcgagctcc gcgcgcgttc cttctgtcgg tgtttggaat tgcggtcggg
    11641 atctcggtgc tcgggttctt tttggcgctg tctttgggca tgcaaaagcg ggtgctccag
    11701 cgaatttttc cgtcggaccg actggaagtc gtgccgccca aagccgcggt ggacgaagga
    11761 gccatgggcg cactgtcggc gctgctcggc gggcccaaag gcctgggcga cgacgtgatt
    11821 cgcgagctgc gagcgcatcc ggacgtgcaa gcggtgtatc cgcggctgcg ggtggcgttt
    11881 ccggtgcgag gctggggtgg cgaacgcatc gtcggtcgtc cgatctacac cgacctgccg
    11941 atcgacggca ttgatccggc ggcagtgacg gaagcgacat cgccgtgggc gttcaaagat
    12001 tacgtggacg acaacaaaaa tccgttttgc accgaggaca aaaactgtcc gcaaggctgg
    12061 ttctgttcgt gggacaaaaa caagtgcgag ccgccggtgc cggtgatcat ttctccgacg
    12121 ctgctggagt tttacaatgg ctcgtttgcg cggatgtacg ggctgcctcg gatcactgga
    12181 tttttggtaa accagctccg cggacttttg gcgacggcag agcttggcaa atcgtttgtc
    12241 tcacggcggg aagtgggcaa gccgcgacag cgcaagctgg tgctggtcgg cgtgtcggat
    12301 cgtgcgcagc cgttcggcct gacggtgccg cttccttatg tacaaaggtg gaatgccgaa
    12361 tacgcggggg aaaaagccgc gtcggagtat tccagcctga cggtgcaggt gaagccggga
    12421 aaaagctcga cgcgggtggt cgaacgaatt cgcaagctgg gctttgcact ggaagacaac
    12481 ggcgccgaac aagcggggct tgcggtgctt ttgatgacgg cgctgtttgt ccttgtgtcg
    12541 ctgtcaacgc tggtggtggc gacgctgaat gtcgcgcata cgttttttcg cgcgattgcc
    12601 gaacgccgtc atgaactggg cgtgatgcgt gccgtcggag cgagccagtt cgatgtcatc
    12661 ttgcttttgc tttcggaagc ggcgtgtatc ggtctttgcg gcggcggact gggaatcgtg
    12721 gtcgcgcgga ttgccgggtt tggcgtggat tatctgtcgc gtaccgccct gcccgacttc
    12781 ccgtttaagc ccgattcgta cttctggttt tcaccgctgc tgttgctgtc gctgatgggc
    12841 ttttcggtcg cgatctgtct tttgggcgcg ctgtttccgg cccgagcggc ggcgcgactt
    12901 tcgcccgcgg aagcgctcac agccaaatag gaacctgcgc catgtcgacc ctgcactgtc
    12961 cgatctgcaa aaaagcgctg cccgacgacc caaatccccc caaaaaagaa gcacgcagct
    13021 acggtccgtt ctgctcggcg cgctgccaaa agatcgatct gggcaactgg ctgggcgaac
    13081 gatatgcggt tccgggcgac cccaccgtcg aagaacccga gccgcgcgag ctgccaagct
    13141 aagccgcccg acgcgccatc gcgagggctg catccgtcga cccgaggcgc gcaggggcaa
    13201 accaggctgg atcattcaca acaggcgatg gcgcgcttgg tggtgcagtc ggacccgcgc
    13261 gagacgccgc catttgcatt gacccatgta ctgccgtatg tggtgccagc ggtgctcgtc
    13321 cagtcgttgc aggcatagct ggcaggaagc cgcgcgtggt cggggacgcc agtgttggtg
    13381 atgtcgccag agaggttgcc gctcgggtcg agccagcccc catcggaagg tggcgttgtc
    13441 gcagagcttg tgcgcaggta ttcggtggcg tggcacatgt gcgcgcccgg aaactcggcg
    13501 gcgcacagcg cgtgcatctt gtgccgtccg cccgcgtcgc cagccgtggt cgcggtggtg
    13561 aagccagaaa agcggacttt ggcgatgcct ttgcagcagg caagcggtcg cgaggacgtg
    13621 cagtcagagc cgcgggtgat gccagccgcg acgttgaccc aggtggaacc gtaggttgtg
    13681 ccggcggtgc tcgaccagga attgcaggtg tagtcgtgcg gatagcgacc aaacttgggc
    13741 gagccaaacg actgaatccc tttgccatcg cctgtcgacg gatcaagcca tgcaccagcc
    13801 gatggcgggg tctgtcccga gtttgatacc aagtactcgg cggcatgaca caggtgggaa
    13861 ccaggaaaag ccgcggcgca gagggcatgc attccggccc gtccgttggt cacggaaccg
    13921 gtatacgtcg tcgcagtaaa gccggcgaag ctggggctct cttcggtgta cgcaccgcct
    13981 gcgcccgccg gaccggttgg gccttgcgga ccgggatcgc ccttgtcacc tttgacgccc
    14041 gccggacccg aagccccggg gtcgcctttg tcacctttgg ctccggctgc tccggggtcg
    14101 cctttgactc ccatcggccc cgccggcccg gtggctccgg tgtcgccctt gtcgcctttg
    14161 gctccggctg ctccggggtc gcctttttcg cctttggcgc ccgccgggcc cgtggaaccc
    14221 ggaagtccct tgtcccctgg atcgcctttg tcgccttttt cgcccttttc gccttttgtc
    14281 ccggccatgc ccgccggccc agcttcaggc gttggggtat tgcagcccag cgagggaaaa
    14341 atcagaccca aacagagcgt ccatatcgct ttccgcatcc gcgtgtctcc cacataggaa
    14401 gaccgcaacc tagctgcaga ttgggacgtt gttcaaccgc caatgacgcg gcgcggctcg
    14461 ctgcggggac cgtgcgatca gctgggcagc tggccactcg ggggtgcgct gggttcgctt
    14521 ggggccaccg ccagcttgag cttgggccca agttcttcga gcagcggctc gacggcttcg
    14581 ccttgcagca cgacccgtcc gagcagctcg taaaagtcga tcatggtgtc aatcgtcgtc
    14641 gacaccagcc ggaacaagtg gtcgtatgac agtccgccat catcggcgcg cggcaccacg
    14701 acgcggtagt acaaaagccg gttgtcgtgg tcgatgccaa aggccggcat gatcaggcgg
    14761 tggttgcaca gcgcgaccgc gtgttcggtc agctgcatgt gctgcggtgg aatcttgtag
    14821 ggaaaagcga cggcaaactg cgcgagcaag agctgttttt cccagcgaat gatcatctcg
    14881 gaatcaagcg ggccgttttt gaccggaaac gacaccactt ggttggcttc gtcgccttgg
    14941 cacggcacgt tttccgattg aagcagcgca acgagatcag caaacgaaag gaccgccttg
    15001 ctttccatat ccacacccca attcatcgtc tcttctaaca aagtgtacgc tttttgccgg
    15061 gccacaagga cagccggcag ccgacttacg gcacaacgct gagcatgcgc gctgactttg
    15121 gccaaccgcg acgcagcacc gacgcatgaa tcatcgcatg ggcaagcgaa ggcccggaag
    15181 tcatcgtcag gaagtgtgga ccgaggcggc ggctggcttc atccagcagc gattcaagga
    15241 aagcgcggcc tagggcacca ggccacgttg cggccaggct cggtcgcagg cgctcgtgcc
    15301 gcggacggac atcgggcgtg acagtcaccc cgaacgactg cgggtcggcg ccaatcggcg
    15361 agcgccgcat cagcaaaaac ggcgtcagcg cgcagcttgc tagctcgtgc tggtgttcat
    15421 caagaaagtc gatggttgcg cgcgtctcgt ctgcggtttc ccctggatag ccgagcagac
    15481 acagcgcgcg gacccggatt ccgatctggc tggcgtcttt gaggatgcgc tcgacgacgg
    15541 gctgatcgac gccttttttc atcagggtgc ggacgcgcgg gaccgccgat tccagaccga
    15601 gccaaatctc ctcaaggccg gcggcatggg ccgcggcaag cacatcggct gtcaaatccg
    15661 gttcgaaccg gctgcgcacc gaaaaacgaa tcgcaaacgg aagctgggac gccacaagcg
    15721 aaagctgaag aagctgtttg ggcgtcagca gatcgtcgcg gaaacgaaaa aacgacgcgc
    15781 caagctgctt gtgcgccgcg atcagggctt gcgaaacttc ggcgacgggc agcgcgcgat
    15841 agccttcctg gtgcatggtg atggcgcaga aggtgcagcg gttccacgga cagccgcgcg
    15901 acatccgcac gtcgacaagc ggttcgggat agggatagag agtcccatcg accagcgaaa
    15961 agtccggccc cggcaaggcc cgcagagatt cccgaccgcg cgaccgcagc gccacgacac
    16021 cgtgttttgg cgccagcagg ctcggcaccc catccagcga ttgaccggca gacagcgccg
    16081 tgtgcagggc ccaaagcgcg gtttcagctt ccccgatgat cgcgccgtcg aagtccgcaa
    16141 aaaaatggtg cagcggctgt ccgggggcgg gcacaagatc gtcgagcaga tcctcggggg
    16201 acagcacgtc ttcgtgcgca ccgtacatgc acagaaagcc gcgatacccg gcggcgcgca
    16261 gcaggcgacc cagcaccagc gtctgcggca gctgatcggg atggacgagc gccacggcga
    16321 tgtaaaccgg atcgtctttg accagcgtcg aaatggcgcg ctgaaggatc ggcagcgcgg
    16381 gattgcgcga caaagacaga cccacttgca gcgccgtcgg gacatgctcg atgtcgacgg
    16441 aaggaaagcg cagcgccggg aaaccatact cgcagccgta gcgcaaagac accgccaaga
    16501 gcgcatcgtc gacctgctga ccagcggtgg catacgcgga cgccgacaga taggtttcgc
    16561 gtttttgcag cgccgcaaag ccggggaccg agcgcaggtg gtgatgcaaa agtcccgccg
    16621 acagatcgat cgcgcgcact ggaatgtcgt ggctgcgcaa aaacgatccg gtgtagagca
    16681 agtccgcagg cacggcggcg tagtaccagt gcggggggaa gcagagcgtc accgggcgca
    16741 gacgggtcat cttgttcatc ataaaccgca ttgggcagcc gccgagacaa acccgcaggg
    16801 ggcgaaagtg ctatcgtgtg gagcttggtg tagtcggaac gtccgaggac aaaaagcgat
    16861 tgggaagccg aagatgctgg aacaagaact cgcaagtgcc atcgcggcag tgcagcgcgc
    16921 cggacaggtg cttttggaag tgtatgcaac gccgtttgag gtttcctgga agggaaagag
    16981 cgacccggtg acggtggccg atgagcgggc caatgcactg attgtggcgg cgctgcgtga
    17041 gcagttccca agcgacggaa tcattgccga agagagcccg gaccacctgt cggcgatcag
    17101 caaacggcgg atttggtatg tcgatccgct cgacggcacc aaggaattca tcgccaaaaa
    17161 cggcgagttt tccgtgatgg tcggcctggc cattgatggg gaagcgcggc tcggggtggt
    17221 gtatcagccc accgaagaca agctgtacac cgggatcgtc gggcaaggcg catggcttgt
    17281 ggagcgggga cagaaaacgc cgctccaggt cagtgaaaag tccgatccgt ggacgctggg
    17341 gcttgtggtt tcgcgttcgc atcgacccaa aagcaccgaa acgctgaccc gcacgctcgg
    17401 catcaccaag gagtccgtca gcggatcggt cgggctcaaa gtgggcctga ttgcgcagca
    17461 gcaggccgac ttgtatgtgc atgtgtcgga caaagcgtcg gcgtgggatg cctgtggacc
    17521 ggaagcggtg ctgcgtgcgg caggcggacg gtttaccgat cttttgggcc agccgtttgt
    17581 ctacggaacc accgagctgc ggacaacgcg cggcatcttg gcctgcaacc aagcggcgtt
    17641 tgccagcctg ctgccgacgg tgacagagat tgggttttcc gacggacttt tgcaaaggac
    17701 ctgaccgctt gatcccggtt tccgataaaa tcgaaagatg gcggatgagc gtgagttagg
    17761 cggtcccctc gatccgctgc cggactggca gctcttcgca agcgtcgcga cccagagcga
    17821 gctgctcgat gaagtggcgc aaaagacgag tggaattgga tcggtcgccg aggctggatt
    17881 tgatgcagca gcggtggcat cggtcagcgt gcccgatcca acgctagacg gcatggcgtc
    17941 ttccgcaaac cccgccgggc cgccagtcgg tttggcgttt gcgacgacct acgcgccggt
    18001 tgtggccaag ccgattcagc cggcgatgac gacgcagcag gcgatgacgg tccagtttgt
    18061 cgatctgctc cgaccgcttg tgcgcagcgt atttttgcga ccgctgccca cgctcgccga
    18121 cgtggcgcca gatgtcgagc cgcaggtacg ccagatcgcc cccacgggca gcgactaccg
    18181 ccccggcagc atcggcaaag caatccacgg cgcgtagata cgcacgaccc aggacgttac
    18241 cggcgctgcc agatgagaca gcggttgtgg gatggcattt cgcaatcctc gacgagcgca
    18301 agaccggcag ttgcggccag ggcatggacg gattcgacat cgcggatgcc tttttgtggg
    18361 ctgctcgctt tgagccagtc gtcgaatgcg gcgttgctgg gacttgtgaa gcggcccccg
    18421 tagttgaacg cgccgtagac gaccagcttg gcgtcatggg tcagcacatt gtgcagcttg
    18481 gcaaacaact gacaaaccgc cgtccacggc atgatgtgca aggtgttggc gctaaacacc
    18541 gcatcgaacg tttgccgggg ccacgtcccg gtcacatcca gttcgatggg cggcggcgtg
    18601 ttcggcagct gcgcttcgtc gagccaggcg gaaatgccgg ccagatgttc ggcgcactcg
    18661 gatgtctgcc agcgaagctg cggcagcgca cgagcaaagt acaccgcgtg ctgtcccgtc
    18721 ccgctgccga tttcgagcac gtcgcgtcgg tcggcaaagt gcttttgcag caccgccaag
    18781 atcggatcac ggttgcgctc acacgatggg gcaaatggct tttccatgag aaatcgtggc
    18841 tacggaaaga cgacgcggtg acgaccggcc agcgctttga ggtttggggc cagtggcgct
    18901 ttgccatctt ctatcggctt gccgtccgat cgttccagca ccagttcctg gccaccatag
    18961 ttcggaacca actgcaccgt gccggcacgc ttttgcgcat cgagtccggg atgtagccca
    19021 atcgaccgag tacagtgcgg cgttcccgac ggacctacgc cacacaccac gaggtctttg
    19081 gtcttggcct cgacgcagca gaacgactgg ccgcgcgctt cgtcaaagtc gcgcatcgaa
    19141 tcgaccccat cgagcgtcac gagcagcttg gcccccggcg gcgacaggat ctcgcgggtt
    19201 tctatggtgg cgcgctcggt ctggcgatag ccggattccc gacacgaaaa cagccgcgaa
    19261 aaatagaaac cgcgcgccgt ccgcaccgcg agtacgcagt cttccgggct gtcgccggcg
    19321 ctaaagacct gcgcttccag ccacggaccc gtcggacggt ccagtttgcc ctgcactgcc
    19381 gcatcgctgc ccaggtgaca cggacctgac tgttttttgc accacgcatc gacggacaga
    19441 agcggtcctt caagcggctc aggcgacagc gcttcgctct gcgcggtcgg atccagatcg
    19501 agcagtgttt cgcgcacgat ccggttttcg cgcagcttga gcgacgcccg cagcgccacc
    19561 accgcccccg gtttgtcgcc tttttgcgaa agcacgcgcg ccaagttgta aagagccgac
    19621 gcacgcagcg gcggttcttt ggcggcgtcg acggcttttt tgcaaacctc ttcggcccgc
    19681 ggcaggtttc cgcttgcgcg cagggtcacg ccaagctcgg acagcacgcg cggctcatcg
    19741 ggaacggctt ggagcgcaga ttcaaaagcg gcgatcgctt cgctgtgttt gcctttgccg
    19801 gccagcgttc gcccaagcga aagctgcgct cgcatcgttt tttgcgccgc tgctgcggga
    19861 tcgaccaccg ccggtgtcgg cattggcttt ttcgcaggtg gcgccgccag aaccgtcccg
    19921 cttaccgaca aacccagccc cacgcagagc gtctgtcccc atgtccgccg catgatttca
    19981 tgggtatcac ggggcagcgc gccggaaaag cccaagccgc ggctagtccg actcctcgcc
    20041 ggtcagcttg agcaagagcc catcgagctg atcgagcgag tgatagtgca cttcgatgat
    20101 gccgctctgc ggggtcttct ggcgaagctg gacccgcgcg cccagtgccc gctgaagccg
    20161 ctgctcgaca tcgcggaccg atgacgacgg ctgtggcttg tctttgcccg gcttgtcttt
    20221 gctgggcttt tgggcacgac gaaccaggtc ttcagtctgc cgcaccgaca gcgctttttt
    20281 gatgacctgc tcggcgtttt cttcgataag ccgcgagtct tccaggccaa gcagcgcccg
    20341 tgcatgaccc atcgccagct gtcccgtcgc cacttgatcg cggactttgc ccggcagctt
    20401 cagcaggcgc agcgcattgg ccaccgtggc ccgctcccgc ccgacgcgac gcgacagttc
    20461 ttcttgggtg tagccaaact catcggacag gcgccgatag gcttcggctt cttcgatggg
    20521 atttaggtct tcgcgctgga ggttttcgac gagcgccagc tcgaacgcct gcgccgcact
    20581 gacatctttg acgacgactg gaacatcttt gagccccgcc cgctgcgaag cccgccagcg
    20641 ccgctcaccg gcaatcagga tgaagctgcc gtcgccgggc ttgtcctctg gggtgcgcat
    20701 gcggaccacg agcggctgca ccaggccctg cgcgcgaatc gaatcggcca gctcgctcag
    20761 tcgctgctca tcgaagcgct gccgcgggtt gtccaccgct ggcaggattt cctcgatggg
    20821 gcagaaaaag aaatcccgac gcgccacctg cggcctgacc ccacctgcgg atgccgatcc
    20881 tgacggatca tcgacaccga cgggtgtttg gtgaacagct gccggcagat tgggaatcag
    20941 tgccccaagt ccacgtccga gcgcttttcg cttgtccatc gcttatcgcg ctccttgcgc
    21001 cgcagtggtc tccgtcggca tgtttgccgc agcccgccgc tgttcgagaa actctttggc
    21061 aagctggccg taggcatacg atccgcgcga cgccgggtca tatttcatga tcggcagacc
    21121 gtgcgacggt gcttccgaca gccgaatgtt gcgcggcacc agcgtgcgat agaccttggg
    21181 accgaagtga ccccgcactt cactttcgac ctgctgcatc aggctcgtcc gggtatcgac
    21241 catcgtcagc aaaatgcctt cgagctcgag accgggattc cacagaccgc gtaccttgtc
    21301 gagcgtcgac aacagatacg acaggccttc gagcgcaaaa tactcgcact gcatcggcac
    21361 gagcacgccg tccgcagcca ccagcgcatt gagcgtcagc agccccagag acggtggaca
    21421 gtcgaccaag atgtagtcgt aacgtccgcg gacttccgac agcgcgtcgt gaaggtgcca
    21481 ctcgcgacgc tcggcgctga ccagttcgac ttcggcgccg gccaagtctg ccgtcgacgg
    21541 aaccagccac agcgtatcga gcaccgtaag ctgcgtcagc tcccgcagag acacccggtc
    21601 gatgagcgcg tgatagatgc cctgttcgac ggaaccacgg acaaaaccaa gccccgatgt
    21661 ggcattgccc tgtggatcaa agtccaccac cagcaccttc tgaccttcct cggcaagtgc
    21721 cgcagcgagg ttcacagtgg tggtggtttt tccaaccccg cccttttggt tggcaacggc
    21781 gatgacgcga cccattggtt tgctctcttc acccagaaac agaaaaacgg gccacaagat
    21841 accgacgagc gggcaagttc tcaaaaaaaa cgacgagata aatttgaccc gtcggaaaag
    21901 cggccatacc agaaggttct gatgcgtctc aaatcgctgt ccgcccgtcc atcctcgaat
    21961 cctcggcaga tcgtgctggc cgccgatccc gaactggatg cgatgctaga tgcactgttt
    22021 gcaagcgagc tgtgcccgac ctggcagacc gccgagcaag ccccggcagc ggagggtgaa
    22081 tcaaacgctg tcgagtctac gacggcagct tgtagtcccg gtattgttcg cgcagcctaa
    22141 gcttgttgag ctttcccgtc gcggtgtgcg gcagctcgct gacgaactgt atgtcgtcgg
    22201 gcagccacca cttggcgact ttctgcgcga gcacggacat aagctcgtcg cgcgtcgtcg
    22261 tttgacccgg ctttagcacc gccaaaagca gcggcctttc gacccacgcg ggatgcgcaa
    22321 tcccaatcac cgccgcttcc tgcaccgccg gatgggcaag cgccgcgttt tccagatcaa
    22381 tcgacgaaat ccactcgccg ccgctcttga tcacgtcttt ggcgcggtcg gtgatctgca
    22441 taaatccttc gccatcgagc gtcgccacat ctcccgtcga gaaaaaaccg tcctcatcga
    22501 caatcgggcc gccctcttga cggaaatact ctttggcgat ccacggtccg cgcaccttga
    22561 ccatgccaaa cgccttgccg tcgtggggaa gctcttggcc gttgtcgtcg acgatcttca
    22621 gctcgacgcc ccacatgatg cggccttgct tgaccttgag gtcgagcttt tcgtcgacgc
    22681 tcattttgtc ccagcgccgc ggacgcagcg acgccgtgcc gacggggctt agctcagtca
    22741 tcccccatag gtgcatcacg tcgacgccca gctcgccttc gagccgcgca atcagcgaac
    22801 gcgccgccgc cgagccacca atcaacatcc gtcgcaggtg tttgggcttt ttgtccggat
    22861 gtttgtccaa gtaacccagc atcccgagcc acaccgtcgg aaccccgagc gacagcgtac
    22921 actcttccga ctggagcagt tcacagacgc tggccccgtc gagatgcgcc cccggaaaca
    22981 ccagcttggc accgacgagc gccgctgaat acggaacgcc ccagccgcag gcgtggaaca
    23041 tcggaacgat cagcaaaatg ctctctcggc tcgacagccc gagcacgtcg gacatacagg
    23101 atgccagcgc gtgcagcacc gtcgaccgat gcgaatacag cacgcctttg ggatttcccg
    23161 tcgtccccga ggtgtagcaa agcgacgcgg cggtgttttc atcgaagacc ggccagcgat
    23221 agctatcgtc gtggccgtgc aaaaacgatt cgtagctgat cacgcctgga tgcccgaccg
    23281 tcgccgcttc gtccccgagc agcacatact cttttaccgt cggtagatgc gcccgcagct
    23341 tttcgatcag cggcataaat gcggcgtcga aaaacagatg ggtgtcctcg gcgtggttca
    23401 ggatgtaagc aagctgctcg ggaaacagcc gcggattgac ggtgtgcagc accgcgccga
    23461 tgcccgacac cgcgtagtac agctccaggt ggcggtgact gttccacgcc agcgttgcaa
    23521 tgcggtcccc tggtttgacc ccgcgtgccg tgagcgcttt ggccagccgc ttggcgcgag
    23581 cgtgaacttc ccgccagttg gaacgcacaa ctccgtcggc atgacgcgaa accacgtcgg
    23641 tgtcgccatg gacagaagcc gcgtattcga tgagcgacga aatcagcagg ggacggtctt
    23701 gcatgaggcc gagcatggtt tcctctcaaa gtcgaacaaa gagccagttg aaatcgggaa
    23761 gttatcgacc gaacagcccg ccgagcccgc caccttgctg aagtcgtttt tgggcgtctt
    23821 gaagctgctg ctgcacattg ggctggcctg gctggcccgg ctgcccagcg ggccgattcg
    23881 tcggcaggtt cgtcggcaag ttcgtcggca agttcgtcgg caggttcgtc ggcaaattcg
    23941 gcaaaccacc ggcctgcccc ccggtctttt gtccaccgag cacattgcca agcgccccgc
    24001 cgagtgcgcc acccgcttga cccccaacgg cacgattgag caggtcttgg ccgcgacctt
    24061 tggcaatcgc cgccgccagc gccgccgccg tcttgaccgg atcagcaagc tggaactgcg
    24121 gctgcgacag cgacccgcca atcttgagtg ccaccggaac cggcgccgcc ggcaccacct
    24181 tgcccgccgt cagagccgag atgaccgccg gctgaagctg caagtcaccc agcaaatcga
    24241 gtgttttgtc aagaccaatc gaaccagtca gctgaatcgc gccctgttcg gtctgatagc
    24301 ggattgggtt gtttgtccgc agtctcccgt cggaaatctt gaactgcgcg gtcagatttt
    24361 tcatcgacat ctcgcgctcg tcgccgctct gaccggacag ccccagccgc tgcataagcg
    24421 gcccgacgac ctgcgatccc agcttggccg tgaccagctt gccctcggta aagcccaggt
    24481 cgagctgtcc caaaagccgc ggcgcaatgt cttcccagcg cagcccttgc ccgtctgccg
    24541 acaggtccat cgagccgtgc ccttgcagct ttttggccag gtcgtttgac ttaaacgaaa
    24601 tgatctcgtg cagatcgact ttgtcgagct tggccttgat cgcaaacttc ggcttgctcg
    24661 gggcaaagtc gaagctcgtg ccagacaaaa ggatgctgcc cccgacggcc tgcatgcgca
    24721 tcactttcaa aatcagcttg ccgcccgaca gcgtcatctc aagcacgaag tcgcgcatcg
    24781 tctgaccttt gagcttgccc gacgcgacct tgatcttgcc ttcagcttcg aggtgcttta
    24841 gcactttcgg cacctgaact tccttgctcg gggccgcgtc ttcgtcgtcg tcgccgcctt
    24901 tcttttcgcc caggatcttg tcgagatcga ggtagtccgc cgaaaagtca aagcttgcct
    24961 gcttcggctc gtcgagaccc tgcacactcg cgccacccaa gatggttccg ccggcgacct
    25021 gcatcttgaa ctggtccagc ttggcccgca ccgagctaag ctgttcggga ttgccgctca
    25081 gactgagtcc aaacgtcagc ttgctgtcga gcagtccgcc cgccgccccc tgacgaagct
    25141 gcggcaccac ctgcgccagc ttcgccagat ccagatcgac cagcggcact ttcatgtcga
    25201 gatacttttt ggccagatca gcgctcccga cgagcttgat aagaccgccg ggcagctcga
    25261 gctgcgcttg tttgatctgc accgccgtcg gtgttttctg cgccgaaagc gaaatccgca
    25321 tcggtacgcc gcgatctttg tcgacgctac ccgagacgtg gagcgccgcc ggggtcaagt
    25381 ccaggttcgc atcgaccgac gagttagacg gcgactgctt gacctctgcc gacagctcag
    25441 cggtgcccga cagatccatg ccgtcttgct cgaagtcggc attttgcatc ttgaccttgg
    25501 cgtgggcgga aaactggtcg ccttttttcg taagcccacc gctcacccgc agatccccgt
    25561 cgatggttgg cccttttttc tttttcttgc cggacgccgc cgatccatcg gccaccatcg
    25621 gcaacagccg caacagcttg tccagatcga cggtcccact gtcgagcgtc aggtcaaagt
    25681 cgttgccact tttcagctcg ccgtggagca agagcgacag cggaccgagc acaagaccaa
    25741 accggtcgac gcggaagccg ggatctttga ccgtccccaa aagtccgagc gtcagcggca
    25801 ttcccgtcgg cttgcgcagc tgtggcgtcg cgatgttgga ctcagtcata tccagcgaaa
    25861 ctttcactgc ggctgccgtg ggagttcccg atgccgaacc gctgaccgaa agcggcccgg
    25921 acagctcggt tcccgtcgga accgccgtct tgggcagaag cgcaagcagc cgctctagct
    25981 tcagaccgtg cgacgcgact tgcagagagt gaaccgccgg acttttccac aggttgcgca
    26041 gatccgcttc ggcggtgacg ttcatgtcgg cgatgtcgag ccgggctttt tccacccgaa
    26101 tgtcacccgc cagcatcgcc aaatcgacgt tcatgctaag catcacgtcc gtcggcgccc
    26161 ctttcgacaa gctgtagagc ttggccggca ctccattttg tcgaagttga agaccgcgca
    26221 gacccgcttt gcccgcgacg gacagcttgc cactatcggg aggcgacttg atgagcagat
    26281 ccgcttctac catcgcccgc tcgatcgtgg ttcccgtcga tgccggcaaa aacgcgagca
    26341 gcggctcaat ttggaatgac tgaagcttca gttccagttt gcgcagcacc gtccacgccg
    26401 gatcgggcct taggtctttg ggcagcggtc ccaccaccat cccaaggtga aagttcggca
    26461 caagctgaag cagcgccata tcgagagaaa gctgggtttc gccaaacagc tttccatcgg
    26521 tgatttgcaa gtgaatgcca tcaatcttca gcggcggcgc ggctttcccc gtcgacaaat
    26581 cgtggaacag caccgccgac tgcgtcagct cggcgcgctc gatggtgatt ccggccagct
    26641 tttcaatttg cgcttgcgtc agcggcgtgt cgtctttggg ctgcgtcgcc aagtgatcga
    26701 gcacgtcttc atacgaaagc cgtccgcccg aaagtcgcgc cacctgaact ttgccattgg
    26761 caatttcgac gcgcgacacg ttgatgcgac gacccatcgt caggatcgcc ggcacgatgc
    26821 tgacatcggc ttcgatgctg ccaatctccg ccagcggctc accaaggatt ccggtctgtc
    26881 cgggctgacc gctgactttg acatcgcgaa cacgcaggcg cagcttcggc aagagccgca
    26941 gactgaccgt gccgaccgac acctcgcggc caatcttttt ttgcacttcg ggaagctggg
    27001 cgacgatctg cttgcgcacc acctggccca gatcgaccgc aaaaccgacg atcagcacca
    27061 ccacgaaaaa cagcagcagc gtgccaccga cggcaagccc gagtattttg ggccagcgcg
    27121 cctgggtctt cactgcggaa ggcgacgggt tcggattgtc cacggacatc tgcgaaactc
    27181 cttgcgacga aaaaacgaag agcaaacggc aggggcagcg aggcaaagcg agcgctagtc
    27241 gttgacgacc agatcgactt tttggccttt gccaactttg ctgcctgcgg ggggattttg
    27301 ccgcaggacc gcaccgtccg cagcattgtc atcggtggct ttgcgcacat cgccgagcac
    27361 aagcccaagc tgttccagct gcaccttggc actgccgaga gaacgaccgc ggaaggacgg
    27421 cagatcgaac tgctcggggg ctttggaaac caccaagctg accgtctcat tcttgcgcag
    27481 tttttcgcct gccgccggtc gcgtcgagat cacctgaccg ggcgggatcg tcgccgacag
    27541 ctcctcgctg accgtgccga gtcgcagccc ggccacttgc agctgctgtt cggctgcggc
    27601 cacgacctga ccgaccagcg ccggcaccgt cgatagctgc ggcggaatcg cgatgaaggc
    27661 atggacttcg cccccgtgtg caacgcgcga gcctttgagt ggccgctgct caaacagcga
    27721 cccggtctgc actttgtcgg cgtcgggaat tttttccccg tccagcacca gcaaaagccc
    27781 gagcggttcg gtaatcgccc gcgcctgctc gacagtcagt ccggatacct gcggcacttc
    27841 gacggtggcc accggcagct tgggcgtgac gtactgatgc atgaccacca ccgtggaagc
    27901 gctcaccacg atgctaacga gggcggacgc gaggtattga cctactgaca taccttctag
    27961 ggtatcaaaa aacctcgctc ttccagactg cttgtcggtt cacgttggca aacagcggcg
    28021 ggcgacgaag cgaaggcggc gcgggctaaa acaggatcgg aatgtcgatt tgcaggacgt
    28081 gatacttgcg gttccccatc acgccgtaga gcccaggctc gtagccgata cccggacgaa
    28141 tccacttgcc gccgggcagc ttgatgtgga aacgcggccc gatgccaagg ctcaggttct
    28201 gccgcagtcc ctgcaccggc aggcctttgt cggcgttggc ttcgtcggca acgaccgatt
    28261 tgggatctga cagccagcgc tggtagcgca gctcggcgcc cagcgacatc cagtttgcaa
    28321 cgaagtagcc gatgtgaaga cccgtcgtga agttgacctt gtagacgtcg gggttcgccg
    28381 cttcgttgcg aacgcggaac agctcaaaga acgtgacctc ggcctgcacc gtcagccggt
    28441 gcccgatgta cgcgagatcg agtccttcga ccatcgccca atcgtttacg gcaaacatca
    28501 cgttgtccat cgccgagcgc gccaactgtg cagtcggtgc ttcggccaga ctcgcgcccg
    28561 gttccgtcgc tgcactcgtt cccgaaccaa ccggaatcgt cgtcccaaag aacagcgaaa
    28621 agcggaagtt gtcggcaaac ttgtgcgaat aaaaaccacc gaggaacagg ttgctggttg
    28681 cgacgccgca cttggtcagc acggtcttcg gctgcatcgt cgtcggatcg gtggtggtgg
    28741 tgtcttcgca gttgtctgcg ccggtttcat cgccggtaat ccgcggcgga cgatttccga
    28801 ccatgccgaa gcggacgatg cccatccacg atggcgtaaa ccgatagccg atcgtcggca
    28861 agaacacccc ggcgaagcca ttttcgtagc gttcgggctg accggctgcc gcgatgttct
    28921 gctgataaaa cgcgaacacc gtatcgagcc gcagcaccgt gacggggacg attggacgga
    28981 gtccaaacgg cagcgagtat ggcggcggcg ccggtggcgg cgtcgaaggc tgtgcaggtg
    29041 gaggcggcgt tgggacaggt gccgcagcca cctgtgcggg cacaggggtt gccacagccg
    29101 ccgcgtcacc gccaggcacc agtggataag gggggggcgg cggaatctgg gtcattccgg
    29161 tggtcggtgg aacgctggcc gggtctggag ccccggtttg tgctgcaccc tgcgcccacg
    29221 cagatgacat cgccatggtc agcgccgata cacaaagcgc cgccgatacc cccgcccgtt
    29281 ttagttgata gcgtctcatc acaatctcct tacccgctgg gaaggagcgt tgacaaagga
    29341 acggattcct cttcttgcag cgcggaagga catgtcatcg gttcgtcatc aagtggctct
    29401 gtttgcccat ccggtgctac gaccggcaag tgacgaccgc caacaggcat ctcccacccc
    29461 tgcacagcga agtctttctg tacccactca cgcagaagat gcgcgagttc tgcatagtat
    29521 ggatggtgcg ctgcgctctg tagcttgctt gcaaacgcag agacccagcg ccccaagtgt
    29581 tcttgcatga aggtcttgcg cgcggcccgc acgatggaca ccgcttcctg tgcttctggc
    29641 gtctcttctt cttggtgaag cgcctctttc agacacagga acgcggcaaa ttccagttcg
    29701 acaccgatgt gatcgggaac ttcggtgtcg cgacccccgc agcgcagacc aaacgcctcg
    29761 tagagcgacg aaagctgtcc aagaccagga cccaagtcac cgcccgtgta cgcacgctca
    29821 tgcggactga ccgcaacgga ttgcgcaaac agacgattgt attcccccgc aagctggtca
    29881 tcgacagacg gaatcagcgc ttccgccagt ggtttgagcg ccgactcgac ctcaagttcc
    29941 tcggaaaaca gcgcgcgcag ctctgtctga tcgggataac ggaatgcagc agccagcaag
    30001 cgatatccct ctgcccgcag cagagcatca cgatcgacgt tcgccgagta ttcggtcacg
    30061 gcagcaccac cttcacaaac ggagcccagt gttttcgacc gccaacattc tggttcgatc
    30121 cgtcccaaac cgcaaacccc atcatcgctt tgcctgaaac cagcatcggg ttggcgcgat
    30181 cgggggactg cctgggcacc gccaaaacca ccgtccactt gccatctttc cacacacctt
    30241 tgccttgcgc gtcttgatag gtgtgatcgg caagcgagcc aaagccttcg gcttgcagct
    30301 cttcgaccgg ccacttgcgg aaaatcttgc tgaccggatt gccagccgca gttcccggca
    30361 aatagcgacg ggcgttgtcg gtctggaacg cttcatcgac cggataggga tagcccccag
    30421 acacaaacgg cggtcggtcc gacacatagc ccggatggta gtcttgcacg tctgcgcgac
    30481 cgcgatcgac atcgcgctgc cacgctgctt tccagtggca gatgtagacc ggattttgga
    30541 catcacccat catcgggttg gtcttgtccg catcaccgag cggaatttcc acagcgacac
    30601 cgtcagtgaa tttgtcgact tcgagctttt catcgagcgt cggatcatcc cactcgagtc
    30661 gcaccgccac aaagcgcgca tcggcgagcg tccgcacccg cagctctttc acgctctgct
    30721 tgaccaggtt cggatacgcc acggtctgcg gcagcatgct gacggcggtt tccggtgtcc
    30781 cattccacgc aggggacagc ggatcagaaa gcggaatctc accgaccgac gtgacgcgga
    30841 tctctcctgt cagcttggaa ggagccccgg tttcatgatc accggacgga catcccgtca
    30901 tcaccggaag cagaaacagc gcaggaaaaa ggaatgttgt tttcatggcc gatcctcgtc
    30961 cttaggtgat gttgagccgc accacgccgt gcttcttgtc gaacatctcg cgcgcaaaga
    31021 acggctccgt aaacggtacg cgcaccgctt cttgcgacat tccgtcgaaa ccgactgcgt
    31081 gatctttgtc ggctttgaac ttgtgaatga tcttgggggt attcccaaac atcaggagcg
    31141 cggccaaaag ctgcttgtct gccggcgcac tccgatatgt cttgagcgct tcttccacgc
    31201 cttgaccaaa catctgctga aggaacagac gcggtacatg cagcggcgga atgtagtaga
    31261 cgttcggctc cgttccaaac tgcggataca gcggcagcgc gatctttttg atccggacca
    31321 agtagtcgat cggattgtcc gctttttctt cgcctggctt cgaaaggaag ccttgcagac
    31381 ggatctttcc gatgcaggtc ttgacgcact gcggctgttc accttgctcg atgagcggga
    31441 agcagccaat gcatttttcc gaaacccgcg tgaccatgtt gtagaaaatt ttcttgtacg
    31501 gacacgcctt cacacactcg cggtagcctc ggcagcgcga ctgatcgacc aggacgattc
    31561 cgtcttccgg tcgcttgtaa atcgctttgc gcggacacgc cgacagacag cccggatagg
    31621 tgcagtggtt gcagatccgc gccagataaa acatccaggt gttgtggatg ttctggaaaa
    31681 acgcaccgcg ctgcacatcg cccgagcaga catcttcgcc caagttcggc gacgtatagt
    31741 cttcttcttc cggaaggaat ccgagcacgc gctcacccgg aggtgccgct tcgaagatcg
    31801 tcttcccgtt atactttccg tcctgccatt cccctggtcc cagcatttcc aggagcttca
    31861 catcccagcc gtgcgggaaa aagccatagg gcttggtctc gacgttgttg tagaagatgt
    31921 gctcctggcc ttttcctgcg gtccaggtcg ttttgcacgc gacggtacaa gtctgacact
    31981 cgatgcactt gttggtgtca aagatgtacg cgatttgttt gtttggacgc gagccctcgt
    32041 aggggtactc catctcgcgg cctaggtgaa tgttctgtac tttgctcatg gccgccctcc
    32101 tacttgctcg aaataaactc gcccttaagg aagcgcttca tcgcttccgt ttcatgcccc
    32161 ggcgtaagac ctttttgcgc cggtctccac agtcccttgc cacccattcc gcctgcttcc
    32221 gctctggtga ttttcacaaa cgactcacgc ggggcgcccg tcggacagta cacatcgggt
    32281 tcgaacccct gaccgacctg ttgcgtgaac gagtttttgc gcaccatcgt gtccgtcatc
    32341 atcgttggtt tgatgaagct gcgcgtgcag ctttggtgcc ctccgtaccg gaacagcgct
    32401 tggtatccgg tctctggatt gcgtgccaga ccgttttggt ttgactcgtg tcctttgatc
    32461 gatccatagg tcgcgccgta catgttgtgc cacatgcgcg tcacgccgcg tggcgttccg
    32521 gggtagtaac ggagccgcac cagcagccgt cccagttttt tgtcttccgc ggtggctttt
    32581 tccggattgc ggaacggacg atcggcggga tcggcatcga tccagacata atcgccgtct
    32641 tcacagccca gctccctggc gtcatcggga tgaacttcga catagccttc ggtgacgtaa
    32701 ggagtccgcg gatcgtggcg cagcatgtcg ccaaacggcc cgaaccacac cgcgatgatg
    32761 tccgtatcaa ccggcgtcgt gtgcgcaccg tgacgatact tcggcgtgtg gaacagatag
    32821 cggaacgccg gatctttctg ctgaagcgga tgcttggtgt tgcgcagctc gtcccacgtc
    32881 ttgacaacgt ggcgcgcttg gcgcgtatcg ccgtccagct gatccagcgg cactccgtac
    32941 tgatccggag tcttcggttt gatcgccgga tgcggcttgc caacaatcac gttcggttca
    33001 acgaacgtcg agtcaatcgg ctcgcgatgc accggcaggt tttcacccga ttcacggaat
    33061 tccttttcat cgcgatacag ctcgatgcga ccggtgcgcg tgtaccacgg cttgtcttcg
    33121 taggcctgtt cccaaccaac aatcttggga taggtacgcg acagaatgat ggtcggaatt
    33181 ccttttttcg cctgctcttc acactccagg aagttcagac cgtgcgccat gttcgacatg
    33241 ttccacacgc gctgcagata cacatcgacg cggccttcat agacaaagtg ccacatgtcg
    33301 tggaaccgct tgtcgccggt cagcttgccc agtgacgccg ccacacccgc cgaacactcg
    33361 atgtcactgc gcgttccgta gatgcggtca atcggcgtgc gtgggaacac cgaaatgaac
    33421 ggattggtga cgctggcagt ggcatcggga tgcttgaact cggcccacga atcgaccggg
    33481 aacaccacgt cagcgtattc acacgaagcc gtccaccacc actcgtttac gaccaccaga
    33541 tcgctcttgg gaaggacgtt gacgaccgtt tcgaagtgtc ccttcgcatt gccgatgaga
    33601 gagttcgagt tcgacaccca gatcgccttc gacggcgtcg ggatgtgtgt ctttccggtc
    33661 agcacttcct tgcccatgtg caggatgtga tcggagtgat tccagtagtg aaccgactcg
    33721 aacttgagga acgacttcgg acgcgacggc ttgctttcat ccagttccac atcgaacgga
    33781 ttttcaagcg agtactgcgg cagtccgtta aagtacgcgg ttcggtagtt tccggcatac
    33841 gaaccgatgt tgccaccgac gaaaccgatg ttccgtgtga gcgaagcaag atagaagatc
    33901 gtgcggtctt tgaggtcgtt gttgaagaac tggtttggac ccattccgca ggcaaagaac
    33961 gtcttttctt tgttcgccgc gatctcacgc gcaatcgtga caattccgtc tttgggagcc
    34021 caggtcaaag actccacggt ttcgggcgcg aagttgtcca tcacatactg cttgagcagc
    34081 tggaacacag gctggcactt gacggttttt ccgtcggtca gcgtgacttc gaattcccct
    34141 tcgagcgctg caaccaggcc gcgttttttg aacttcttgc cgacttcatc gcggctaatg
    34201 cactctgctt tgccggtctt ttcgttccac accacaaatg gatcgaactt cgccagcatg
    34261 tcttccggaa tgatcgcgtc cttctgttgg aaatccggtg cgggcttctc tccctttttc
    34321 agaatctgcg cgcccagtgc accagtcagc ttgattccgg cgacatcttt ggcgttcagt
    34381 cgttccagtg tatcgagccg aatcagcgac ggaagatccg tgtacgaacg cacgtattcg
    34441 gcatcgtaca ggttctcttt taagatcaca tgcgcaaacc cgagcgcaag tgccggcgtc
    34501 acacccggac gaaccacaag tcccatatcg gacttgttca tggtcgcgga atactctgcc
    34561 gcgatcacga ccaccttggt tcccttcagc cgcgcttccg tgagccagtg cgcatcgggc
    34621 atcttggtgg tgatccagtt catgccccac acgatgatga gcttggcatg ctcagccatc
    34681 gacagatcca cgtccaccgt ctgctgtccc gtcaccattg ggtggcccgg cggcagatcg
    34741 gtatgccacg tatagttgtc ccagccgcgc gcgcccagcg ctttgtctgg acccactttg
    34801 cggacaaagg catccagcag cgcgagcgtc tgtgcgaagc gatactgacc gaaaatgcgc
    34861 gtcgttccga gcagcggcat accgccacgc atcttgatca cttgcgttcc gacttcatgc
    34921 gtcgcttcga ccatgtgcgg atcgtagccc tgcgcaagca gccacttctt gccctgctcg
    34981 ccgctgtagc ttgccgccac gttgtgcatt cccttggcga ccagatcgaa cgcttcatcc
    35041 cacgatacgc ggacaaacgg ttccttgccg cgctgaaggt atttgggatc gacttttccc
    35101 gtcgcgggat cgcgtgggaa tccgtccttt tgccactgga gccacccctt gcgaaccagc
    35161 ggatagcggc agcgccgatc gccataaaaa cgccgtacca gcgcgagtcc tttttgacag
    35221 cagcgcggat cccagcgatg cgacgacttc attccggtga gatccaccgc ctcgccatat
    35281 ccgaacgtcg gaccgatgcg aacgatggtt ccggaacgaa cgtaggcgcg caggatgcag
    35341 ttgtgcgtgt cgttcggagc gcacgtaaaa gtgaacgtcg aatcgtaggc gtatagatcg
    35401 cggtagatcc gttcccagcc gcgattggga tacgacttca gtggattgtc gacgctcact
    35461 ggctctagga agttgaacgc ccagcccttc ttgcttgccg tcgcagcagc gacacctgcc
    35521 gtaccaccca aggcggccag gaatgaacgg cgggaaaacg ttgacgaaga catgttgctc
    35581 ctccccttgc ttggggtttt cacttaaagg tcaagccgta gctaccgcga ccacaaacag
    35641 gtccaacgag ttgcgtgttt cgaatcaata tgagtcattt taaataccta tttattgata
    35701 caatgacaca gccacaccac ctgcacacac accaaaagat gcatcaagcg cgcttcatat
    35761 tgaagacaca aaaacaaaca ccgtcaccat ttccgcaaat atgaatcatt ttaaatacct
    35821 aattttgaac aaaaaaatgc cgcttgtacg actctcacgc cgagcccccc gtcgtgagag
    35881 tcgttcgcag cttacttggc gagcccgcgg acgtgcgcag ccagcgcgtt tagcacttcc
    35941 gtcttttccg cgagatccgg gttcggtggc atcgccgggc tcttgcccac cgccgcacca
    36001 ccgctcttga tgatgctgac gatgtagtca tccttgacgc tgccctgcca cgccttgtct
    36061 tggaagttgc gcggcttggg ggtaaggcct gccgatccgg cgccatcgcc cgcgccctgt
    36121 gcaccgtggc acaccgcgca gcggctggcg aaaatttcct gtgcttcctg cttcgcttgc
    36181 ggggaaatgg cgggagccga accgctcgcg ccacccttgt tacaggccgg ggccagagcg
    36241 agggacaaaa gggcgaggct ggcgaaaacg agcttacgag tcatgtgtct acctcttcag
    36301 gttctctttc actaggggcg ctttgtcatc ttcaaaccga gcaatctcgc gcagcgtggt
    36361 gcgacggaga aattccgcca ctcgctcgcg ttcctcggtc cagaaacagt gcgccggaca
    36421 gttgcgttcg tcgctacaaa ccgtagtgcc gagcatacag cgcttctgca cgatcggatc
    36481 gtcgaagacc tcgcagattt ccagcagcgt cacgtcctcg gcagcacgcg ccaaagagac
    36541 gccaccgctc gtcccccgct gggtcgtaag gtagcccttg cgcgccaggg tgtggacgat
    36601 cttggccaga tacgcgggcg ggattcccgt cgctttcgcg atgtcacgca cgaactgggg
    36661 ccctggctgc ttctggaagc ggctcaggtg cccaagcgcg gcgatcgcgt atccaaccgt
    36721 ctggctcagc atgtctaagg cgatttagcg accaaatgac catcatgtca attgacttgt
    36781 ttatcttttg ataaccatat cgctttgtcg cgagccgagt cgtcggaacc gctccgcgcc
    36841 gatgccaaaa ccccatcgat ctcgtccgcc ccacagccga gacccgacgt gcgggcaaaa
    36901 aaaaacccgg ccaaagccgg gtttgcgctg ggtgagtgaa ggggcttgaa ccctcgacgc
    36961 ccggagtcac agtccagtgc tctaccaact gagctacact caccagaagc gacagaagtc
    37021 tacgaagaac ccactttccc tacaagcaaa aaatgcaggc tttcgtgggt ttagccaagc
    37081 acggcggcaa actgatcgac ggcccagtcc aggtcttcgg cgctcacaac gagcggcggg
    37141 gcgatgcgca acacaaagtc gtgggtttct ttgcacagga cgccccgctc ggcgagcgct
    37201 tcgcaaaatg gccgcgccgg gcgatcgagt tcaattccga ccagcagtcc gcgtccgcga
    37261 atttcgcgaa tgtacgggct cttgatctgt cgcagtcggg aaagcagcgg cgcccccagc
    37321 gtcgcggcgc gctggcacag cttttcatcg tcgagcaccc ggatcgccgc ccgcgccacc
    37381 gcacatgcca acggattgcc gccaaaggtg ctgccgtggc tgccgggacg aaagacatcc
    37441 atcgcatccg ccgagctcag caccgccgac accggataga cgccgcccga cagcgctttg
    37501 cctaaaatca cgacatcggg acggacgttt tcgtgctgac aggcgagcag ctgtccggtg
    37561 cggccaagcc ccgtctgaat ctcgtcggcg atgagcagca cattgtgctg tttgcatagc
    37621 gccgccgcag cagccaagta tccgtcgggt ggaatcaaaa taccggcttc cgcttgaata
    37681 ggctcgacga gaaatccgca agtccgtggc gtaatcgccg cttgtagcgc cgccagatcg
    37741 ccaagcggca ccgtcacaaa accgggcgta aacggaccaa agccatcgcg atacagcgga
    37801 tccgacgaaa aactgacgat cgtggtggtg cgcccatgaa agttgttggc gcagacgatg
    37861 atttcggcct gtccgtcggg aatgcccttt ttttgataac cccagcgccg cgccagcttg
    37921 atggcggttt ccaccgcttc ggcgccgctg ttcatcggca gcattcgctc gtacccgagc
    37981 agctcgcaca gctctttttc aaacaagggc agctggtcgt tacgaaacgc ccgcgacgtc
    38041 agcgtcaccc gctgggcctg ttcgacgagc gccgcgacga tgcggggatg gctgtggcct
    38101 tggtttaccg ccgagtacgc cccgaggcag tcgagatacc gcttgccttc gacgtctgtg
    38161 acccaggcac cgctcgcttt ttcgatgacg acggacagcg gcttgtagtt gtgggcgccg
    38221 aagcgctctt ccaaatcgac gaagtgttgg gtcttttcgg gaagattgcg acgcatctgg
    38281 gtcatggctt cctcagattt ccgtgcgggc ggcggcgact agaacggcgg caatttcggc
    38341 ggcgtacttt tgggccaggg cgacatcatc ggttttcagc gacacggcac cgacggcagg
    38401 atgcccacca ccaccatagc gcccgcacaa gttggcgatg ttggcggtgc gacgctcggc
    38461 gcgccaagga ttgcttccga cgctgatctt ggtacgaccg ggcagcgaag acaaccccac
    38521 gacgtagtga gcgtcgggga acaggtaata cggcgcgaac ttgctgacca ggatctgcgg
    38581 cacatcggtc acgtcgtaag tcaccacacc gtctaatacg actgctctct cgtgcagcac
    38641 ccgcagcact tcgtcgcgca tttttacgac cggcgcgatc ccgtcttgca catacgactc
    38701 ggcggcgatc tcgtcgagcg actggctgcg cagcgccgaa ataaaccgta gcttgagctg
    38761 cggatcgcgg ttttgttcca cccacgtcat aagctgaagc gccggttcgc gacacatcac
    38821 cgcttgttcg gggctgacaa aacgtgctcc gtcgatgaga tcggcccacg caatcagatc
    38881 ggcaaacggc gtcggatcaa aaccgaacgc gctgctacag caagttgcca aaaatttgct
    38941 gcacgactgc gctttggggt cgtaaaagtg ccgccggttc tgcgcggcgt gaaagtgcgc
    39001 ttggtcttgg tcggacagaa acgcgctctg gtggtgatca aaccaccacg acagccgcga
    39061 atcggtcgaa tagcgaaagt cgacgcagac gtgttcggtg tctggtccgc ctatgaacgc
    39121 gccagcaaac acatcgcctg gcccgtggtt catgcccacg tattcaaact gggcgtcagg
    39181 cagcacgcag cgacggtaaa attcggaaaa cagcgccgcc gaggtggcgc cgtcaaaaca
    39241 gcagtcgtgg tacagaacgc gaacgagcat gaaggcctct tccgacggaa tgcagcggga
    39301 cgggagctag atgcggatgt gtttccagtg gccgcgacgg aattcgatga acatcacgac
    39361 ggccagcatc gcaatgtaga aaatcagcga accccagacg ccccacatcc cgagtccgaa
    39421 cgtgatgccg gcgagccacg acatcggaat caggcagcta aagtgaagaa tgccctcggc
    39481 gaccatcacg aacatggtgt tgccggcgcc atacagcgct tgggtgaaca ccagcgcgac
    39541 gctaatcaaa ggacagaaga aaccgagcac ccgcagaatc ggtgcaccga cggcgatgac
    39601 atccggatcg gggttccaga agtgcaggat cggttcggcc cacgccgcca aaatcaggcc
    39661 cagcaccaaa aacaacaccg cgccgattcg cgccgcttgg tacgcaaaac gctcggccag
    39721 gtcatattcg gttgcgccca tgttctggct gaccagtgtc gccgtcgccg tcccgtatgc
    39781 caggcacgaa atgaagacga tctggaggac gttgatgatg ttggaagtcg ccgtcgaaaa
    39841 gatcgtgcga ccgacgccgt gacccgccat gtgatcgagc cgcgacacga catacagcac
    39901 aaagccgaag ccgcccatcg caaacatcgt cgcaattccc gacggcaccg acagcttggc
    39961 aatctgccag atcgtgctcg cggaagcgtt ggtcactcgc ctgatgtgga acttgcggta
    40021 ttcgccgcgc agcgaccagc caagcatgat gaaaagtcca acatacgagc tgagcatcga
    40081 cgcaaggccg gcgccaggca cgcccatgtg caccagcttg tcgccaaaca tggacagcag
    40141 cagcgagtga atgccgtcga caactgggat tcctggctga tcgggcccat agatgaacgc
    40201 gatgcacatc aggaagttcg cgatgttcat cacaatcgcg acgcccatgt ggacgcgggt
    40261 tttgccaagt ccgtcgaaaa acgacttgag gctcgcggtc cagaccatcg acgtgatctg
    40321 caaaaagcgc cagcgcagaa acggcacacc gagcgcaatc accgccggat ctttgttcat
    40381 cagacggaac agcttcggcg ccatcgcata cgtaaacgcc gtcacgatga tcgacgcgac
    40441 gacagcaaga agctgcgaat tggtcgatac cgcgccagcg ccttcgatgt ctccggcccc
    40501 gatgcgacgg gcagctagcg cttgggtgcc gacgctgatc gccgccagca tgccgccaaa
    40561 cgcccacagc aaaatgacac tgatttgcag cgccgtttgg ccgggggtcg actcggcctg
    40621 tggcagatgc ccgacgagca catggtcgac ttggttgata agggtctggg tgagcatggc
    40681 caacatcacc ggagcagaca gcccgaggat gcggcgggtc aggattggat cgaagaatga
    40741 catgttggag tggttggcct cgaaggtggc ccgcaccgta gggtgcgacg gggcggtttt
    40801 agcgtgcccg tctatctttc acaagcacgg agatgtccat tatgagcgga ccccgaaagc
    40861 attgggagtc caccgtgaag gcggcaacgt cgcggtcagg tggtcggatc aggatcgatg
    40921 ctgccttccg gcacctgcgc aaacagcgca cgctttgccg attcatcgaa tactccgtcg
    40981 gacagatacg cgccaaacgc atccagatac gacagtgact tttccactgc ggacgcaaac
    41041 cgaccgggaa agtcgaagtc gtcatagact tcggcgcggg cggcttcgcg ctgcttgcgc
    41101 ggacctatcc gcggtccgac gatggcgttg ccaatcaggg cgcagtactg caaatatccg
    41161 gcagcgtatc gtcgcagatc ggcgcgtgtc gggccttgtc catgcactgt gcacagcgcc
    41221 gcttgcaccg cgtcaccgat cgctccatcg tcaccgagaa tgcgcccatc cacttcgatg
    41281 tatcgctgga gcttttccgt ccccatcagg tcggcggaaa agcgctgttc gtggaaaatc
    41341 aggctgtgga acttttcgat ctcgtggtgc tcttggctgg gacaaagccc cgatcgcgcc
    41401 gcacgccgtc gcgccagcgc attgatgtgc gggtgcatcg acgtatcgac cgccgcgtgc
    41461 gaaacataac caagcgcgag cgcggtgagc gtcttgcccg cttcttccgt cgctgatttt
    41521 tgcgcaagtc gctgcccggc ttccccgaaa agccgcccga gcgtgatcgg cgtcttttga
    41581 tgaaacagct cgccaaagtg ggaaggggcc tgcggacgac gcagcgcata acgaaccacc
    41641 gcgacggcaa aacgatcaaa gtacggcaga tcgacgaaca tcgcgccaag ccgcgccgcg
    41701 tcgtgattca ttcccgaggt tgcgcgccga acccacgcgc tggctctcga cagcgaatcg
    41761 acgagcgcag actgatgcac cgcttccacc ggcatgcgct catcctaaac gcggacggtt
    41821 tgcttgcggc gcagattgcg acatagtggc gcggtttgtg ccgcgcgtgc gcggacgatt
    41881 gggaagtgaa ctgacatgaa agcgatcttg attgccgccg gcatgggccg ccgcttgagc
    41941 ccgtacaccg acgatcggcc caagtgcttg gtcgaaatcg ccggcaaacc gatgatcctg
    42001 caccaaatcg ccaacctccg gcgcgtaggt gtcagcgact ttgtcgtcat ccgcggctac
    42061 ctgcgcgaca aactgaccga cgtgctgtcg cgcgaaccgg gcgtccgctt catcgacaac
    42121 ttcgactatc caaacaacaa cattttgctg tcgctgatgt gcgccaaaga cgagctgtcg
    42181 tcgggctttt tctgtagcta cggcgacatc gtgtaccgtc cagaagtcag cgaaaagctc
    42241 gcgcagagtc gcgccgacat cggtctggtc gtcgatccct actttgccaa ggtgtacgaa
    42301 ggccgcaccg agcatccgat tggcgaagca gagcttgccg aagtcatcga cggcaaagtt
    42361 cgccgcgtcg gcaaaaaagt ggtgccaact gctgaggccg ctggggaatt cattgggctg
    42421 tggcgcgcgt ccgctcaagc cggcgtcgcg ctgcgcacgc tgtttgaaaa acgcctggcg
    42481 gaactggggc tgtcgtcgcc gtatggacgg gcgcagcggc tgcaagtggc ctatctgacc
    42541 gacctgttta acgacctgat caccgaggaa gcgcggatcg gcatttccgt cgacggcgtg
    42601 atgattgacg atccgcagtc gtggcgcgaa atcgacaccg tgcaagacct ggagcgcgca
    42661 cgcgccgtcg tgagctggca atccgccgca ggaggttgag atgaagatcc tcatcgccga
    42721 tgcatttagt gaagcgcacc tgagcgcatt ttcgcgcctg gggctcgatg tcgattaccg
    42781 tccccaacta tctgcggcgg agctaccgac cgcgatagct ggcgtcggca ttttggtcgt
    42841 tcgcagcaaa caggtcagcg cggccaccat cgacgcgggc agcgcgctgg cgctgatcgt
    42901 ccgtgctgga gccggtgtaa acaccatcga tcttgacgct gccagccgtc gcggtgtgtt
    42961 cgtcgccaac tgcccaggtc aaaacgccat cgccgttgca gaactgacga tgggtctgct
    43021 gctcgcgctc gatcgccggc tgcccgatca agtggccgag ctgcgcgccg gcaagtggaa
    43081 caaaaaggaa tttggcaaag cagcgggtct gcatggccgc acgctcgggg tgattggcac
    43141 cggatcgatc ggccacgcgg tgatccacag ggcgcaggcg ttcggaatga aagtgatggc
    43201 ctggtcgcgc agcctgacgg cggaacgtgc gcgcgagctg ggcgtcgagc gcgccgagac
    43261 cattccagcg ctgtgtgccg caagcgatgc cgtcaccgtc catgtggctt atgtcccagc
    43321 gacgcgcggg ctcgtcgggg aagcagcact tcagcggatg cgcaaaggcg cgatgttcat
    43381 caacacatcc cgcaccgaag tggtcgacac caccgcgctg cgcaaagcga tcggcgaaaa
    43441 aggtctgcgc gtcgccctgg atgtctttga ccacgaaccg gaaaaagcgg aagcgccgtt
    43501 tgccgatgac atcgtgcagc tcaagggact gtacgggacg catcatgtcg gcgcatcgac
    43561 agagcaggcc cagtccgcaa ttgccgacga aacagttcgg attgtcgatg cgtttgttcg
    43621 cgacggtacg gtgcacaact gtgtcaacat cctgccgccc gatgaaaccc cggcacgggc
    43681 ggaattggtc gtgcggcact acgacaaagt cggcgttctg gccgagctgc tcggagcggt
    43741 gcgtcgtcac aacatcaacg tcgagacgat gcagaacacc attttcctcg gtgccaaggc
    43801 ggcctgtgcg cgaatccgtc tcgcggtccg gcccagcagc gagctggtca gcgaaatccg
    43861 tcagcacccc gacatcatcc acgtcgacgt catcgagcta cccgagacat gatccagatc
    43921 gattctcttt cttctgcttc acggctgccg gggcttgccc acgttccgat cgtgccgcgc
    43981 gcggtgcaaa ggctgcgcgc cggcattccg cttgtcctgc gcagcgacgt ggccgccgag
    44041 ccttctcccg atagtgaaga agtgctgttg tgtgatctgc gcggacagcc gattgccacc
    44101 gcgctgtggg tcgcgcaagg cccgctggct gcacggctgt ggtctctttc gggtgagtcg
    44161 ttttcggaag agacgatcgc cgagcggctc aaagcggcgc ttgtgcgacg gcgaccgatc
    44221 ttgcaaggcg gcgagcgcga tgccttccgg ctggtgcatg gcgaagcaga ccttttgccg
    44281 ggcctgtttg tcgactgtta tgccgatgcc gcggtgattc aaactgcgac ccgcgcgatg
    44341 gatcgccaca aaagcgaaat cgccgatctg ctcttgcagc ttttgccgat tgtccgggtg
    44401 ctctttcgcg atgatggcag tgcgcgcgac atggaatcgc tgccgcgtga aaagggtttt
    44461 ctgcgcggcg gaccgctgcg gatggcgcgc tttcacgatg cgggatcgac gatggaagcc
    44521 gacctgttgg cagatcgcaa gaccggcagc tttctcgatc agcaggaaaa tcacttggaa
    44581 gtcgcgcagt gggcgcagtc gatctgtcca ggtggggagg cgcttgatgc cttcacctat
    44641 cacggcggtt ttgcgctggc gctcgcgcga gccggacttg gtgtgattgc ctgcgatgaa
    44701 gatccgcaag cgattgggcg cgcccgtcgc aatgccgagc tatcgggtgt cgcagtcgat
    44761 tttcacgtcc acaatgcgtt cgatctgctg cgttcctatg aagcggcggg acggcgtttc
    44821 gatgtcgtgg tggtcgatcc ccctgccctt gccaagcgcg gtcgcagcgc cggatctacg
    44881 caacatgcga tgacacaagc gatgcgcgca tacaaagagc tgaacctgcg cgcgctgcgg
    44941 attttgcgtc cgggcggact tttggcaacg tgtagctgct cgggccgtgt caccgtcgaa
    45001 gattttgccc agatgctacg cggtgcggcc caagatgccg gtcgccccct tcagcttctg
    45061 tggcgacggg gcgcagcgat cgatcatccg gtgctgtggg gcttgccaga aagcgaatac
    45121 ctcaagtgct ggctgtttcg ggtgctggcg taggaaagtc tagtattagg acttttgcat
    45181 gactcttttt tgcagccgct gcgactctgt gaccaaaggc agagacgacg gcgataggtc
    45241 gggcaaaagc ggcagcgcaa cagactctcc actgatcgat ggacgcgcag catgttggcg
    45301 attccgttcg agcaaaagcg tcaccgcgcg gctcaagcga accagtatgt cccgctggtt
    45361 ctgcgacaga gcgcgcggct gacgatcaat gacgcacagc gttccgattt cactgccttc
    45421 gctcgtgata agcggcgcac cagcataaaa acggatgctc ggatcgtttg tcacgagcgg
    45481 attgtttgca aaacgcacgt ccttggtcgc atcggcgatt tcaaatactt cgctgccgtg
    45541 gatcgtgtgc gcacagaacg cgacatcgcg cgacgtctct ttcgcatcaa gtccgacgat
    45601 cgctttgaac cactggcgat gttcatccac caagctgacc acggcgatcg gcgtaccaag
    45661 gatgagcgac gc
//
LOCUS       DAORGN010000013        44649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_279_length_44649_cov_5.882159, whole genome shotgun sequence.
ACCESSION   DAORGN010000013 DAORGN010000000
VERSION     DAORGN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 44649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44649
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_44649_cov_5.882159"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKL17_02860"
     CDS             <1..696
                     /locus_tag="PKL17_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK43694.1"
                     /translation="KASFLATVSHELRTPLTSVIGYTEMVIEGMAGPISDEQKEYLDT
                     ILAKSDQLHQLISKLLDVSRLEVSSVQLARSPVDLKVLLGDVVRSLSPQLRRKRTRLV
                     LSISDQLPTLQADRDKLRQVLLNLISNAIKFTRDEGEVDVAAEPAKSEGGDLRVLLSV
                     SDNGIGIPTDLHDKVFDAFYQVDNSSTRQHEGSGLGLLLVRRYIAAHGGKVWIEDRPQ
                     GGTIFRILLPVSG"
     gene            complement(715..2091)
                     /locus_tag="PKL17_02865"
     CDS             complement(715..2091)
                     /locus_tag="PKL17_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNK43695.1"
                     /translation="MSQTDPQSPPSSSPQTAPGRPALPVNSRSASDVLAEMQAMRSRD
                     ARWRDGRTFSLVYHDTDEHLQLLRDAYSLFLAENALNPMAFPSLRKMETDVVAMCSGL
                     LGGDQQVVGSMTSGGTESLFLAVKTYRDQARKERPQITAPEMVLPTTAHPALLKSAHY
                     LGVKPVLVPPGPDFRADVAAMAQAITPQTLFLVGSAPAYPHGVIDPIEALSDLALSRG
                     LPLHVDACVGGFLLPFVRKLGFAVPPFDFAVPGVTSMSADLHKYGFAAKGASTVMYRT
                     SELRRHQFYVYSGWPGGLFGSATVLGTRPGGAIAAAWASLNALGESGYLRIARTIMDT
                     TRAFIDGISAIPGLFVLGKPDCGVFAFASSGPDLMAIADQMEDRGWHMDRQQLPTAVH
                     LTITPAHAPIVDRYLTDLRETTAYVVEHPELSSQGTAAMYGMLMHIPDASMVDDFILT
                     NFDALYRP"
     gene            complement(2170..3075)
                     /gene="yedA"
                     /locus_tag="PKL17_02870"
     CDS             complement(2170..3075)
                     /gene="yedA"
                     /locus_tag="PKL17_02870"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HNK43696.1"
                     /translation="MKFRAHTLRIILALVAVYVIWGSTYLAIRFAIDSLPPFTMAGLR
                     FVAAGLFMFTVGKLSSAPMPTRSEWQGAALIGVLMLVCGNGAVCWAEKTVPSGLASLM
                     VATVPLWAVVLSTLLPGGTRPAPRAIVGILCGLVGVFVLVQPQKSGSIDPAGAAALLL
                     ASVSWAAGSVFSKRVKLPSSPVLSTAMQMLCGGSLLLLLGSLRGEWSQIDPQAVTLKS
                     ILSVGYLMLFGSVIGFTAFVWLLRNTTAIVATSYAYVNPLVAVLLGWAYNGETPSSRT
                     FVAGLMVIFAVALITTAGPPKAKTA"
     gene            3278..5569
                     /locus_tag="PKL17_02875"
     CDS             3278..5569
                     /locus_tag="PKL17_02875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43697.1"
                     /translation="MASDAVVLDKRFQLGKVLGRGPSGVVHLATDLSRGSSCVVKRIH
                     AKYSNRNILDRVQALSHAAADMMHPVVLGVLYTGYEPSGTLYLVSPFIDGESLKARLA
                     RGPLSLTATLAMLEPLCSALQAAAERDLAHGALHPSNVFFPTEGGVLLSDFGMAELRV
                     SAGAKWDGPLSYVAPESLETGAAPATVRADVFSLGVLVYECLIGQPMFDAKSLASYLA
                     MVGTPPKMTVKHPSYAHLDAVLEMAAQADPEARFATVQALWRALQSALLDLPASLANN
                     LTRGMAPSAQRTASPSKPPQARKPGSKPSVPPRPPSGAPAPPATAPRPESGSNPPARP
                     VQAMPQLLIEEEIEAPEPLEPSGALVAPARPQPTKVPPKAQLPVTQKLPEPLPPPPVS
                     AKAAATAPVGPVPVAAPKPALAAARPTAAKAAHRPVAAMAPIPFGDDAAGAAATGVRA
                     PGPPRPQRLDPQQILPTDPSYRLRTPPPSKLGAMAPLFWATIGGIVVGGSMIGVQYYS
                     RLSARPQAVHSAATTIDIEGLLRQAETESLSRNYASAFAYTDLILRTQPSHTAGKAVA
                     EQASEQLRVSAVYGAFLRAADREQSDLAVALYRELPPGSPFRSQAWDPFMKVRNSFVR
                     RRLAVATAALTAAECDGIREQIEKLYFISDSATDSALQQGQRLLGKCKSGQGTEAIAE
                     AHIERGDKTDKPEKTAKADKAAKAEKTDKPELVAVNDAGETEKKKKRKAKDKPSTDKP
                     SDGAAKPKEEPKESLPSALRNPF"
     gene            complement(5566..5805)
                     /locus_tag="PKL17_02880"
     CDS             complement(5566..5805)
                     /locus_tag="PKL17_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HNK43698.1"
                     /translation="MLDAERRREIARLGGKTAHANGNAHRFNPEEASLAGKKGGAVIS
                     ENRDYMANIGRRGGLAKRGYKLRRAQQNEVDTQSN"
     gene            6320..7816
                     /locus_tag="PKL17_02885"
     CDS             6320..7816
                     /locus_tag="PKL17_02885"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNK43699.1"
                     /translation="MLGNAQTFLDNEDSLAQSQPEEFPALMEPDPVAESTERAQRGLL
                     EAMTALEDLRDLAPGQKRALRSFLKELGTIYQALGGKSRTPQRHAQLARLLRDRRLAL
                     GMSLEALAKRIGVSASTLKQIERGVRPPSRKTLLLLQNAIELELNLDGILAKESSAKP
                     GASLNCYIAPGYEPVKMVMELSEMLNGPGGYLEQTSAYLEHQSALQFLRMANDPAYVS
                     AFRAGNPLDRIAKVISENTRGMGIEMIALGAGDAREEVRLVQDIVARMEIPDLRLYLL
                     DISQPLLSEGFKHAADVLCSQQGVSYFAMQANFHHMERYPQLHYTPERSHRRRLVTLL
                     GRTIGNLDNEPKFFKYNLVGCQEGDLLLMDFQLAYASTSDLAAIRRREPVLSGGRAQG
                     QRAREDWLASAIWRHCRDVTDVKFHYDLLTDCSVPGSYSIEAVATVHARGRDPRRFSL
                     FRFRRYDPDQLASCLSGLGWDLVEDFPRPQPQGGGDCWLLFRRAKQPK"
     gene            complement(7848..8666)
                     /locus_tag="PKL17_02890"
     CDS             complement(7848..8666)
                     /locus_tag="PKL17_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK43700.1"
                     /translation="MTQRPLGASTFGIITGAASGFGRALAIELGRRGGRLLLGDINLS
                     ALEETAKLAREAGAQAVTTVRCDVSKLEDVQALRDAAGSTPIDLVVNNAGISSGGLVG
                     EIPIETWRKTIDIDLWGVIYGCHVFVPALRQQGHGAILNVASAAGLLAAPRMGAYNVA
                     KAGVVSLSETLAGELTGTNISVTVLCPTFFRTNIAADGMFEDPRIKRMAEKLVERGRP
                     VEEVVKKTLQAVEAGQLYCVPMADGRWLWRLKRALPSAFRALIEMNDRLMAKKR"
     gene            8762..9433
                     /locus_tag="PKL17_02895"
     CDS             8762..9433
                     /locus_tag="PKL17_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK43701.1"
                     /translation="MDRSPSRRKAGAQVADAAVRKPTTSRAAKRPQRARLLVDERRAQ
                     LLALGIKLFAERSYDEVSIDELARAANISKGLLYHYFPTKRDLYTAALRQAAQQLLDE
                     TQPLACLPQDERARRGLETYLAFADRHGPAYVALMRGGLGADREIVGILEATRGTIAQ
                     RILDALPTGIATPLIRAAVRGWIGFVEATALDWLAHKQVDRDRLVELLLGLLFDAIAR
                     AAVAG"
     gene            complement(9380..9901)
                     /locus_tag="PKL17_02900"
     CDS             complement(9380..9901)
                     /locus_tag="PKL17_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43702.1"
                     /translation="MFESIRDAGSQGISRLWQRIRPGALASALSTVLHDLRFSDGQGQ
                     LWPLRVLTSDEKLSKPLKAAGCSVVVAQSAAQSPVGIAVDALCVLLKQRAQLDDLTAL
                     LATVRSDGMVVLCSRSGQLPRELLSATLLHAGLIDVTQKSAGRHLLTAGRVQPRLPCD
                     RGPRDGVKQKPEQ"
     gene            complement(9915..10691)
                     /locus_tag="PKL17_02905"
     CDS             complement(9915..10691)
                     /locus_tag="PKL17_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNK43703.1"
                     /translation="MSTDAFFHPLTRGLLLFLAGAVASGLNTVGGGGSLVTFPTLVAL
                     GCSPLVANATNSASLWPGSLGSALGLFGPLAAAKHQIPKLLLPTVMGALLGAQILTLG
                     GERVFGWAVPPLILLATGLLALQPTIRAWQKQRRQIPTWLGLFLQFLIATYGGYFGAG
                     MGILMLALFGLFVDGDIHAHNAVKAWLGLLINLLASLLLLRQGLVDLGSALYVSAGSV
                     IGGYLTARVMQRIDPTRLRRLVVGLGLVLGIWFLARIVRV"
     gene            complement(10700..12289)
                     /locus_tag="PKL17_02910"
     CDS             complement(10700..12289)
                     /locus_tag="PKL17_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HNK43704.1"
                     /translation="MNQGLPKRPTFPETFNMADYFLDQRIREGRGDKVAVYDDHGSYT
                     YAQIVERSEQVCGALWQADIRPEDRCLIGLPDCVEFVESFFGILKAGAVVAMVNPDLP
                     TEDYLYYLRYTRCRALIASADLVIRIAPLLPECDCLRAVLIVGDCDMDLLAAARIPSH
                     IQVLTWDLVQSSPRRGTYPTSKDDPAVWLFTSGSTGKPKAAVHFHHDFPYNTECYAKN
                     VLGLSAADRTTAVSRLYFGYATGTNLMFPFAVGGSCVLFRGRPTPEVLLDRISRHQVT
                     VLASVPTALQRLLDHDPDAKLSSLRLITSAGEALPAELADRFFRTYGVEILDGIGSAE
                     MFHIYISNQPGNVRPGSLGQLVPGYAARIVRPDGSDAEPGEIGTLWVEGDSAALCYFQ
                     AHEQSKTTLRGDWVVSGDLFHRDEDGFFFYDGRADDLLKVGGIFVSPLEVENVLTGHP
                     AVVECAVVGEENQHGLVRALAYVVIRSGHSADDSLADALIQHCKQKLAHYKAPSRVEF
                     VSDLPRSDRGKILRRVLRRGN"
     gene            12424..15684
                     /locus_tag="PKL17_02915"
     CDS             12424..15684
                     /locus_tag="PKL17_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43705.1"
                     /translation="MGRNTVRALHRRLLCSWLRYGWLLVLLGLPRLAAAQSLGQAELR
                     MGEPAPRGQQWSPLRELAKLEYPGLWEYNWGDSPRDALGRLVYSIPETPTRDWQPLGR
                     YELPKGRNGRRWLWLRSEIPAWASTLKDPVLQMPHLDQEFQVFVEGKLVYEWGDFSTD
                     KPSYLGYRGHIVRLPPGCGGKRLTLRIYSDHVNIGPAGPMRFGDHGEILVNLFKTDLP
                     RLIVGGLLFLLATMGFALFSFRREEKVFLTYGFLALSGGIYILAQAQVRLFLFDHPLL
                     LTHIELWSLQITMLALCHFVESMFGSGPLRILYYLRQLQWAYLFGSALAVALGLVPVL
                     KTLLPVQLMLFGDAALSVALPLYYSFRGNIEARIFATGFLGAGAIAVYDVLVALGVLS
                     RTQPKLGHIGYALFFVTLGVILARRVLRAHQRLQNYTSILNLSLASSRVLVPDERAQV
                     ALSELVRLLSARRAFLFLWNEQSGQLEFATGRSNDGQSLGAAEGCDLGFVEQSMTAQK
                     PAARRSADKKVVQMVAPLEARGQKLGVLYIESASTRQGLHEEDLDVLIGLAHQVAISI
                     LSARTLRIEVDAAMAQKRMQEQQILLDAAVRMAAGDLSTPVTVPSGSTMTHLAEALEA
                     MRTDLLTKVLEMESKGREVQTLNEELRRQIAERSRRLMSVIIRSKAGKSTPLPDLSPG
                     KVVVGRYRVVRKIGEGAMGVVHEVERISDGRHLAAKFVPQLSDKKSLLRFMREAQILA
                     RLRHPNLIGITDVDMTAGGILFMIMDLVVGGSLKSARRRFGEVRWALCVLRQIAEALA
                     AIHTAGIVHRDLKPDNVLLAGGEESALPFVKLADFGVSLLMSKVQTSPLSRALTQPIQ
                     AVVDPLEESTEMQVPGPDWFGTEDRDVDLGEDGFADTADIPVQPARTTGGVLVVSGQP
                     SAEEGALTLQLPPGGRTTGPVGAPRESTGSAARKNSSPDSLAATGESSVSGVQGPVTQ
                     SGILIGTPMYMAPELWHSGSHRAQPSSDLFSFGVIAFELLTSVLPFLQPPVFAYAQKQ
                     TLRIAKLATSRSELPPKLCALVDSCLSENPAARPSARVVVEQLRELGAPTPMSSSG"
     gene            complement(15681..16682)
                     /locus_tag="PKL17_02920"
     CDS             complement(15681..16682)
                     /locus_tag="PKL17_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43706.1"
                     /translation="MSDYEATPYDGHHTQGDSEHSWRTNPVERFVGYGNWAGPGNRMD
                     HEEADYIAQQRKQNPNYDPYVDPHMLNNPRYRAVDGMDAAAQRHDHGYYQHLGADSGA
                     ADMFTWQGLQATREDDRRLVADTRAEMAQNGNRYSADAQSFGRGLQGFFGARVMGQDA
                     VDWAGNRAQEAASGVGNFVNGARNWNSLGDAASGIGQGIRGAGNWLANTGREAWQGVS
                     GAYNNIRGLGWEGALGATGGFLNVAGAGAAHLAGQAWDGARSLGGAAVNGVANAAGTA
                     GRAIANGASAVGGAIANGASAVGGAVANGASAVASGARAAGSAVAGGARRVWNWLTG"
     gene            16826..17860
                     /locus_tag="PKL17_02925"
     CDS             16826..17860
                     /locus_tag="PKL17_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43707.1"
                     /translation="MSGRRLWIGLAVGAVGLGSLLWARSKDTAQSSSATSGEKAAPNS
                     SDGAAKTGWTRAHRLATQLDHPKGVVVDGEFAYVVTGGFAQAINAVLRISVTSGAVET
                     LVKVPQILSGELAIDDKYVYFSSEADNRIFRVSRIGGPAVTVCETIRPIHLAVDETHV
                     YFASLKSETADGAVLKISKLGGQPQVLVAGQTGVDQLAVDRDALYFRSNSGLWRVGKQ
                     GGAADCLLPAARGKNVDHLVADETHLYFFWETSGNGKYRVARMPKRGGAPEAIGPIAN
                     PTGRLALSDSHVYFFRAADLTDDALAKVEKQGGEPALVDGSGYSTGYLTVSLGDVYFT
                     DVNSLYRVPK"
     gene            complement(17840..18994)
                     /locus_tag="PKL17_02930"
     CDS             complement(17840..18994)
                     /locus_tag="PKL17_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43708.1"
                     /translation="MWQLVLGVTFALAGWLALLVWSGWVRPLLRLREQVLALSRGEWN
                     RLLLSEPLPGELWTVLLRLEGLRRALLDRLRSSTELNLQLEGEVARRNADLARRNSEL
                     QQALVSLGRARDELLHRERLAAIGSVVASLTSEINNPVGSMVALLEPLCETHHELGDK
                     LLTTAQDDTIADDVRTEALAMLTELRQMVDLLQRGGRRAHDVVRAMRGYVSSGTTTQT
                     TVPLLPLLFDLYQLFAERVPLTVRLRDGGATQTFDLRKPPRQQPAITVQATRSDLSLL
                     ISRWLLRTVPRLLPEQRAQIDIELLRMPVDTPSVVLRLADSGAKLDTLSLSLFDSQAA
                     SVGLACQVTTDRQGEKDEAMDLTTELALKLPLVCAQKEDVTAHELLGNAI"
     gene            19100..19849
                     /locus_tag="PKL17_02935"
     CDS             19100..19849
                     /locus_tag="PKL17_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43709.1"
                     /translation="MKSTLFSACALVGAMAIPAAASAQNCAVHFAAGFGSVSGSVAKA
                     TTQPAADVLVDQAFGPRPQHCEAGAYTSFLEQFENFGRTAIRTAQPTRGSKAPIAPVK
                     ENQLRLAISIVNKMSPQKVPLADSKTAPSQWRQVRSNLNAVADDAGNTPMMVQFLDAV
                     GRISPPGPIEEVVTVGQGAPSGSSVATPGISAPPGGVQQVRVPLVPLPSWAVIKLYEA
                     RDLCKPQDIAGIQLRLQDVINWMESNTQPPQ"
     gene            20066..20956
                     /locus_tag="PKL17_02940"
     CDS             20066..20956
                     /locus_tag="PKL17_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43710.1"
                     /translation="MNTYRQIRANTLSGFGFSLVMGIASLVGCSGATDTTPTPTPAPP
                     DMALQYSYTPATINQIDTDPGNGPFGNAVRVVLDRVVAVSKVDKYVNSANQQCRYQIW
                     VQDPACTVPPCGMVVKAIGPMAPSATSTGKDCPAASASGTLLDTISRGDNVRVRGKIV
                     VEVDSTTPMTVVEHQLFVETLDILTPDKSINPVVITDPLVFAQFVTHKGTTWNKYEGM
                     SVTLQPTTGKLQIATINGGGFTTTPGNTDWGNTFDSDYFPSGSTAFPTVGSTYTSISG
                     VVSTRHGGEIMPGRNKDFVP"
     gene            complement(20989..21960)
                     /locus_tag="PKL17_02945"
     CDS             complement(20989..21960)
                     /locus_tag="PKL17_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43711.1"
                     /translation="MSLLARILLAALALGHDPTPLRVPESLLAITSMPEASGVVWNPT
                     LSRYLVVSDDTGLKEKGTNHAPWIFALSREGVFDETPIPIAGIEKLNDAEALCSGPDD
                     TLFLATSHSENKKGHVKAARRRLFQLTLVGRTLKILGSFDLAGAIVEAGVIPSDSVDI
                     EALSYHGGELYVGLKAPQTASGAAIVLKVRDVLSAMKDGAISAGRISKFADIPLAVEA
                     AAGGGTVTEGISDMGFLPDGSLLILGNSPKKRPPDGGGALWWRKPDGSVVMLRRFLGL
                     KPEGISLSDDKKHIVIVFDNDRKLPLWLHIPIPKTTQAAVPQKKPSQ"
     gene            complement(21944..22930)
                     /locus_tag="PKL17_02950"
     CDS             complement(21944..22930)
                     /locus_tag="PKL17_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNK43712.1"
                     /translation="MALAGFWYLSHGFTVGPGTTIGYAEEVIHQIAPLQTARLREVTV
                     VLGQKVKAGDVLARLDDKNLQLKRTRVVAELDQVKANLVAEQDVQASFLMRGQIQAVR
                     AYADVQRMRAELREYSGQLKRLNTLKRDQLVRAAEVEAAQRQKKSLSADLSSRPLGTV
                     RDQELMGLRPRPKTDQDRRLEERLGPFRNAVSVKEAELAELDQQLADLVLKSPVDGTV
                     GTIVLRPGDVVGAGTPILTVVTTRPGHVVAYVPERQVTNFPLGRAIVLRKTGVVVTPH
                     YGTVVELGPLIEEVPIRSRPTPSVPAWGRRIVVKVDKPMELLPGEAFRVTPR"
     gene            complement(22983..23636)
                     /locus_tag="PKL17_02955"
     CDS             complement(22983..23636)
                     /locus_tag="PKL17_02955"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNK43713.1"
                     /translation="MDTRIGWDITLAALLLAFALSMTISWVYVATYSGLSYMRSFANT
                     LAIAGVVSALVMLAIGDDVARGIGLVGALTVIRFRSTLKDTRDLMFAFVALSVGVACG
                     VLAFAVAMLGTAVFCAAMAYLSFSDFGTRRMWNAVLRLQVATGENQQKTLSKVLQKYA
                     PSFVLINARDLGGGTQEHSYHLRLRDASEKALILQSIGEIPGISGATLLMQDTTIEP"
     gene            complement(23638..24441)
                     /locus_tag="PKL17_02960"
     CDS             complement(23638..24441)
                     /locus_tag="PKL17_02960"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNK43714.1"
                     /translation="MKAAPISFERFEYKYWLTDAQVQAMRTLTAPHLRCDDFALGGQR
                     NTSLYLDSDDLDFLDLHVQKTPDRPKLRIRAYGNPPSGNAYFEVKRKIKRVTFKDRAV
                     VPMDAVPALLRGEIPKGLKLKNPAEMKTLEHFLYLMIVFKASPKVLITCSREAFTSID
                     SMEGVRLTFDRDICYQPARGPTLQGDPKAWVHLCGQMNYQPEAPTLAELKFKNIAPRW
                     VSDMVQRLQLQLSACSKYVMAMSLEALGGDGLIGPDLAQAAPPPQGERV"
     gene            complement(24438..25787)
                     /locus_tag="PKL17_02965"
     CDS             complement(24438..25787)
                     /locus_tag="PKL17_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK43715.1"
                     /translation="MWRGVVFVSVLICCAQVFAAPKAAKKPAMAEPQTQSDAQSPLTK
                     SQEVQSAKVEAEADGLTEDEAVLRALRINPNLRAFRRQRGVAEGQVVTASMITNPAFQ
                     LQLVHVQTGADMGAAATLKWAPPQPIVYAANRSLARAHLEQVDHEIAEQEWLIQNQVR
                     STYSLLLMLKKQLAVCEQMIGLRKRVLELYKTRLSHGGATRIELNQVQLSLLAIERDR
                     DALLLRQVDAQSDLRTQIGEVTASPVKLRGAFPVDLEHASLPSADALAEQALAARPAL
                     KAAHSKIIQRQHALRAERAKAWPWFEISARYRHTTSTNYPDEVLLGIEIPLPVLNQNQ
                     GPIQEAAAQVDLALGLAEAQFLILKQSVFNAYAALQVRRSVLLRYEREVLPILAEHEQ
                     LLELSLRGGQIDLVTLLAGEETALRAQRDYFDARLAFRIAWLALQAAVGQPLEESRS"
     gene            complement(25969..28686)
                     /locus_tag="PKL17_02970"
     CDS             complement(25969..28686)
                     /locus_tag="PKL17_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNK43716.1"
                     /translation="MNGTDVPPTPPPTMAKPSVLFSEVMYHPVMENAYVENHEFIELY
                     NRSDAAVDLSGWQLTGAVQFAFPAGTQIAAKGYVVIAKNKAALVSVSSYNLSTDRVLG
                     DYAGELDNGGAVVNLVDPSSVTQDSFQYTDQFPWSIGADALGADDEWLALLPTPITSA
                     PHQYKGISLQRVSYDVPTSELSNWVPSPLDGANPGQANGQSGPPPTIVTRKTVTPTSG
                     NLVIGSADTVKVSVVFSTLAPFTKPQLQWFVDDLQATGEPVTTVDLQNNNGFYEATVP
                     KQPNNSIVRYRILVDKGSGSEVISPRPSDPLTHWAYFVSPPAASTAPAYNVFIKKENW
                     NQMYDNVNWPTPDDKRVTPGGSSANRCMLRASWDAMVPAVFVSDGVVYDVMTRYEGSR
                     WNRTNGIGFDITKTTINPRADRPANTILSWKVDFPNYAQFEGKRGKMVLNKLNQACPG
                     LDDAVGQLIYGDPSVNVPVQRAKFVRLNLNGGYYHYMLDIEHIDGDMMKRYRAPGERV
                     GDLFKADGNAGAVEGPWGVSDETTLGLNTTCPQWTIDDRYKYTYDRKTYNWDNNKKLQ
                     TTIETLNTLRAAAVASGDYTALKAFLVANFDTQKMIDYIAIRNWAEPWDEGFHNHFLY
                     QRASDGKWLLIPQDKDREWGEALGWQGGRSFFWGEEGNGQYNRWKDAFIKVFRNELRA
                     RLLELDATGVLNPASWKSKVLQAATTFSISDYVASPAAASICNFYTELQGMQTFGDYR
                     HNDVLDFGDEATCTATTCGLKADMYQTAAGDTTRDFAKATLKTSKVTPTVNYDWTSGT
                     PDPLVPADNFQIRWTGKVVPRYSETYTFFTQADDGVRLWVNGVQVINNWATQTGTVET
                     SGTIALTAGTPVTVTLEYFEGTGNAAAKLLWQSASVYKQIIPTGRLRPM"
     gene            29005..30888
                     /locus_tag="PKL17_02975"
     CDS             29005..30888
                     /locus_tag="PKL17_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HNK43717.1"
                     /translation="MAKFRLIDRLRYSLDATLSRGPGGLIGWLFVLGGLLALLSAAFI
                     TATASAPAGPSGVRPGFFLLFWQGFQKTLNLNVGIGSLLYVIGLLLPTLGSLFIGGIF
                     IGLLTGGIQNRIRNLRKGRSMVIEKGHIVILGWSPQIFQLIGELVLANANKRDACIVV
                     MGEKDKVEMEDIILEKVPSLGSTRIVCRTGSPIDLIDLALVNPDDARAIIILAPDVKD
                     PDAQVIKTMLALSRRQGSAESGCPIVAEIRNPRNRPVAELVGKARVKVLLVDEIIARI
                     TVQTCRQVGLSAVYAELLGFDGDELYIAQEPQLVGKRFDEAMRLYEDSTLVGIQREGK
                     SLFNPPMDTQLRKDDAVIAISRDDDTVLLAKQKPPSPNQAAICAPVSHGESPERTLII
                     GWNRRAPLIVKELDQYVAPGSFLLIAAELADGDVVMEKQRQLLKNQTLQFCSGDTSDR
                     AFLDGLDAHTFHHVITLGYSDRLGPQEADAITLVTLLHLRDLAERHGNPYSIVSEMYD
                     PRNRELAEVARADDFIVGDKLVSQLLAQLAESPQLQLEELFDADGSEVYVKPAGDYVR
                     LGEDIDFYTVMESASRRGEVAIGYRILSEQQSRHYGMHINPKKSDRITFSAQDKIVVF
                     AHS"
     gene            31048..32793
                     /locus_tag="PKL17_02980"
     CDS             31048..32793
                     /locus_tag="PKL17_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNK43718.1"
                     /translation="MSCTEGGSELPLDAIPILPGGTSSVIQTKSIQVGPAGDAYEQEA
                     DRMAETLTRSGSSSGSLSPAQSSPLLTSPQGILHRKSEDTASASGQTTQLVGASKGQP
                     LPQDLKQEFGSKLSADLSGVRIHTDAQSHAWNRSLFARAFTSGQDIYFGKGEYQPNTQ
                     EGRKVLAHELTHVGQQNPGLLGAPVLSDPSRLVRPLRINPKDKKYYSDVAPGLHSEYC
                     ADTAEDSFLTATSNAGLGGGHTAIYIEYFKKENGTDVPRNLRVELFLGPRGSGIDISI
                     RDYSSQDMQAKLFELGSPHKKTWKKKRSDAESALKKAEDIQKGQADYTYYRLGRGPTL
                     KNPVLNCARFGQRVLQAAGIDLHIGSAKLPSRITDPTRQNGAISNVYTSADLAADAQS
                     SLPDCCRKRSEFLTAAKRDQNDRFGSALAAAGGFLYQYHQHPESLLRYPALVGAIDQI
                     HDYVKSTSIEEFVEQRRDSTATVPGSASLDFIRKELHPALQSEVERLRKEVSAELKDL
                     DDAGAPRGAHAKIDSALKTAMSRLDELMAFSTSKELVAARVKNTLSKGGQLQDRKYNQ
                     IMTQGEYPALIANWG"
     gene            complement(32853..33395)
                     /locus_tag="PKL17_02985"
     CDS             complement(32853..33395)
                     /locus_tag="PKL17_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43719.1"
                     /translation="MKFTTFRNLIVGGAVSGLFGSMYYFAKSETVPPPKPAAPVAVAA
                     PVAPAPPTPPPAPAKEEDKPAPKAEAEKTTASGHAIVPLRPFDDEIIHLLSQPPSADK
                     AKDATPHRPYKVNLYAEGGKYVRAKVDLNRNGKWDEKWAVEEKNGEPVVKRQVSPSDD
                     DKNYPDKFKLRGKAWEKTDP"
     gene            complement(33392..34291)
                     /locus_tag="PKL17_02990"
     CDS             complement(33392..34291)
                     /locus_tag="PKL17_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43720.1"
                     /translation="MTLAPPIPAEGVVSWNINTACNYRCSYCTQRFMEDRGRWSRDTP
                     RFLAGFAKLPGRWEIKMSGGEPFVHPTIHEITAGLAEIGHRISIVTNFSASVAKLREF
                     VDAARGRVGVFSCSLHREYVTDVAAFIQKAQWMRDTLLAARDESLPPPSLNVTTVATR
                     QAIPDLVHLASQFAQAGVTFKIQPEKIDGGIIEYSEQEREQLVSLGGHNRTGQLAHVY
                     TGEPCWSGARYFILDDRGVAYRCYPARRHKMERLGDFLSDSFALFLAPRPCPYPLCYC
                     TVPIERGLMRKPSPAGNHAQGQT"
     gene            complement(34303..35247)
                     /locus_tag="PKL17_02995"
     CDS             complement(34303..35247)
                     /locus_tag="PKL17_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43721.1"
                     /translation="MTRSPTIEWQVCGICNYDCSYCIQSKVYRQGHPSPAEVEQFLDF
                     FAQLKTRFEIKMTGGEPFAFRGFMDRIIPGLIEKTPHTISLLTNLSAPIGYITRLAEL
                     TQGRLGVVSASLHLEFTTVESFVEKALILKQTMDPRAHLSINSVLVPGRIAQVIAARD
                     VLERAGLTLFPQIMKVDGGIATYSAEEQALIRDLVGEDPTPDRANMAPSYKGRLCYAG
                     VEYFVLLQNGDGYCCRTARRHKQGFLGNVFSGTLTLWETASPCPYGICPCTVPANRGM
                     IEGIRKAERKYNEDPKSHAEDLIPAQSLRMSQRAMQSR"
     gene            complement(35244..36317)
                     /locus_tag="PKL17_03000"
     CDS             complement(35244..36317)
                     /locus_tag="PKL17_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43722.1"
                     /translation="MKRVDPRLLGMHLGRPVTGPDTLHIDITNGCNTNCVTCWDHSPH
                     LTVPRPQRWKRQRIDAATVTDILNEVSELGGLRAVILSGMGEPFTHPEVYDMIAEVKR
                     RGLHLTIITNLVAAQPQRIVDLGVDQLLIGLHAASAETYRAFHPSFVNDEWQRVLAML
                     AQFREAGRRFKHIHVICRENAHELVAMIRQAAEYKAEQVNFKLASLREGTEAVRISDE
                     QRQKLMAEWLPAARQEAERLNVTANFAVFADQLRAGEEATAPIEDIGCFMGYSYARIL
                     VDGTVLYCCSPEVVIGNLSDGSFPDLWDGPRWNQIRAQLRRGEYFPACHQCGKFNMNH
                     TLSQRFAQAFSQERLYQVTGRKP"
     gene            complement(36314..37966)
                     /locus_tag="PKL17_03005"
     CDS             complement(36314..37966)
                     /locus_tag="PKL17_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK43723.1"
                     /translation="MPSPLRIVNYAVNGSGAGHLTRLCAINRWLRRYTQVLDLRAEIY
                     FLTTSEADSLLFSERFASFKLPSKTIIGDTGIDKTTYLALAKQWVWHSLGLLRPDLFL
                     VDTFPRGSFGELLSALDLCQHKAFIHRSVKEEFLSRPDFAAMLPLYDLILVPEHEEES
                     QGTLKGIRRGQLQYTGPVMIREKVECWPRDKVRAHFGVAEHDLLVYVSAGGGGDRTAS
                     LHLQKVCEALRLTPNLHLVIGAGPLYRGPSLHGPRMTFLTQSGVAELLSGMDLAVCSA
                     GYNSFHELLYAGIPTVFLPQDKIADEQDKRADLAEERGAAQHLRIPIDSPGFLYKLSA
                     AVDSYLDPSVRRQASLAAQSLVPHNHARDAAQKLLALLVPGPKVMAAAEAVDDLLIEQ
                     LAESGMSIEQVCEIAHVLRSTHGETSELIPCEEALSLLRLSHQYALPQPALVRMVRQL
                     AGRIGTGGLDEKSVAMGELLQSLAGFSDWPGAQSLLKQLPQERSLPTADAVAELRRLL
                     GTLRSRQLDLYEGVKALSVAQSDDQSGGSLSDLVTSAIRKLS"
     gene            complement(37979..39406)
                     /locus_tag="PKL17_03010"
     CDS             complement(37979..39406)
                     /locus_tag="PKL17_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43724.1"
                     /translation="MAAYFSSVTYVHDAQKARDQMKETLAQLNTQQQAARREVSSLVN
                     RQETALSELAAAMLPSLTPESIEAAIGLSGYRTFAIVNPAIELQKAQHKLGEQIRDRA
                     VQIERRAHDIERLPEFENRLLLRAPRTGTLTREIAELEEFREPLCEVLRRCEHPRMER
                     LLESKYGLPEYSVPFWRLSYFSDWKAGDEIVAKFGGEKTFVDVRSEYLAARDGVAVYD
                     QKLSKLRAEVERGAAIEREHDSLLAEQKALSEGRHGLQEQLAKMPEQFLKEARIQLER
                     YLADLDFVTIGDRLAQHHDVETMAKRYFGLRKQAEYLRQTSTQLLDNSRPALEQTIQK
                     LDREITKYQRPKYASQRISADQLTKIAERNRRSQEVLARQRDAQTAILAFGAYEMGRL
                     DEDFLWWDLITDGKVKASYIDEVARFKALHPTYRYRRPKQNKSGIVEDVDDDDLIVDD
                     DGVIGDAVIAVIGSGNHHERSSDYS"
     gene            complement(39413..39757)
                     /locus_tag="PKL17_03015"
     CDS             complement(39413..39757)
                     /locus_tag="PKL17_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43725.1"
                     /translation="MLTESQGSAVLRESFLAAGYNIHDNVPFSEDGVAFHVDGFDPVA
                     RVGFEFITTEAGDRTELSQETIAKIEQRIARGELFILLVDEYDVATEDELRQYAERFL
                     DMVATRRRGNRE"
     gene            complement(39817..40869)
                     /locus_tag="PKL17_03020"
     CDS             complement(39817..40869)
                     /locus_tag="PKL17_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNK43726.1"
                     /translation="MSTTENPQVKPALSRKDFVSNQEPRWCPGCGDYSILAQVQKVMP
                     EFGIPKEKIVFVSGIGCSSRFPYYMNTYGFHTIHGRAPTIATGIRLARPDLTVWLATG
                     DGDALAIGGNHLIHALRRNIGVKIVLFNNRIYGLTKGQYSPTSEMGKKTKSTPAGSLD
                     HPFSTLSLAIGAEGSFVARSFDTEIQHLQAMLKRVGEHKGTTLLEIYQNCPVFNDDAF
                     VHITDKTHKADRVLYLEHGKPLLFGKNKDKGIRIGKNFVPEVVDVGDGAGQVPLSEIA
                     VHDETIENPSYAFMLSRMEWPNYPVPVGVLRNVSRAPYEDLVTAQREQMQASRGKGDV
                     AKLLRSGETWVIQPDN"
     gene            complement(40887..42776)
                     /locus_tag="PKL17_03025"
     CDS             complement(40887..42776)
                     /locus_tag="PKL17_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNK43727.1"
                     /translation="MENQTQSGETAPKPPNPPAMQVVESVVIRFCGDSGDGMQLTGTE
                     FTRASALALNDLATLPDYPAEIRAPAGTLFGVSGYQIHFGSQEVYTPGDQPDVLVAMN
                     PAALKTNLPDLVPGGILIVNTGAFGQKNLELAGYSSNPLEDGGLDKKYRLIQIDISKL
                     TGLAVEGSGLGNKDAGRCKNFWALGLMFWMYQRETERELRSIQEKFAKKPELAEANIR
                     AFKGGYSYGENTELFAQTYHVRPAKIEPGLYRNVTGNSATALGIVCAGQLAELPVFFG
                     GYPITPASDILHELSSFLNYGVTTFQAEDEIAGVGSTIGAAYAGSLGVTATSGPGVSL
                     KQEAIGLAVMLELPMVIVNVQRGGPSTGLPTKTEQADLLQAIYGRHGECPVPVLAAMT
                     PSDCFFAVIEAARIAIKYMTPVFVLTDGYLANGSEPWKVPELSSLPKFPVQFRTDPNG
                     YQVYGRDPETLARAWVRPGTPGLEHRIGGIEKHNLTGNISYDPENHHQMVKTRAAKVA
                     GVAVEAGELITCGPPEGDLLVVGWGSTYGAITQAVQTMQTKGHKVTSVHLRWLNPLNP
                     RLAAYLKGFKRVLVPEMNNGQLVRILRAEYLVDAEGLNKIQGKPFKVAELCQAIAERC
                     PGGQK"
     gene            complement(42895..43965)
                     /locus_tag="PKL17_03030"
                     /pseudo
     CDS             complement(42895..43965)
                     /locus_tag="PKL17_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
     gene            44202..>44649
                     /locus_tag="PKL17_03035"
     CDS             44202..>44649
                     /locus_tag="PKL17_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43728.1"
                     /translation="MVIYDPSTGKRKSVCAETRGAADLLAVQLRRETETKPVLTVYEA
                     LAQWIDEKRAAWRHPESVARNVENRVKAWIPDVPVDEIDAAQASALYLAATKSEGRFG
                     PIKAATHHGYLKVVKELWRWLLDREFIEKDAFAKVKPIGKANAGKQQ"
BASE COUNT         8778 a        13385 c        13410 g         9076 t
ORIGIN      
        1 aaagcgtcgt ttttggcgac ggtctcgcac gagctgcgca cgccgttgac gtcggtgatt
       61 ggctacaccg aaatggtgat agaaggtatg gccgggccga tcagcgacga acagaaggaa
      121 tacctcgata cgattttggc caaaagcgat caacttcatc agctgatctc gaagctgctc
      181 gacgtgtcgc ggctggaagt gtccagtgtt cagcttgcgc gcagccccgt cgatctgaaa
      241 gtgctgctcg gcgatgtggt gcgcagcttg agcccgcagc ttcggcgcaa acggacgcgg
      301 cttgtgctgt cgatttccga tcagctaccg acgctgcaag ccgatcgcga caagctgcgc
      361 caagtgcttt tgaacctgat cagcaatgcg atcaagttca cgcgcgacga aggcgaagtc
      421 gatgtggcgg cggagccggc gaagagcgaa ggcggtgatc tgcgagtcct tctatcggtg
      481 agcgacaatg gaatcgggat tccgaccgat cttcacgaca aggtgttcga cgctttttat
      541 caagtcgaca actcgtcgac gcggcagcac gaaggttcgg ggcttgggct cttgcttgtg
      601 cgtcgctaca tcgcggcgca cggcggcaag gtgtggatcg aggacaggcc gcaaggcgga
      661 acgattttcc gcatcctctt gccggtgagc ggctgatcgg ggacttcaga cgggctaggg
      721 acgatacagc gcgtcgaagt tggtcaggat aaagtcgtcg accatcgacg catcgggaat
      781 gtgcatgagc attccgtaca tcgcggcggt gccttgcgac gacagctccg ggtgttccac
      841 gacgtaagcg gtggtttcgc gcaagtcggt caggtagcga tcgacgatcg gtgcgtgcgc
      901 gggcgtgatg gtcaagtgaa ccgccgtcgg aagctgctgc cgatccatgt gccagccgcg
      961 atcttccatt tgatcggcaa tcgccatcaa atcgggacca cttgacgcaa aagcgaagac
     1021 gccgcagtcg ggcttgccca gcacaaacag ccccggaatc gccgaaatgc cgtcgatgaa
     1081 cgcgcgggtg gtgtccatga tcgtgcgagc gatgcgcaga taacccgact cgccgagcgc
     1141 attcagactg gcccacgcgg cggcaatcgc accacccggt cgcgtcccca gcaccgtcgc
     1201 cgaaccaaac agtccgcccg gccagcccga gtagacgtaa aactgatgcc gtcgcagctc
     1261 cgacgtgcga tacatcaccg tcgacgctcc cttggcggcg aacccatatt tgtgaagatc
     1321 cgccgacatc gatgtcacgc cgggcaccgc gaaatcgaat ggcggcaccg caaacccgag
     1381 cttgcgaaca aacggcaaca gaaatccacc gacgcaggca tcgacgtgaa gcggcagacc
     1441 gcgcgaaagc gccaagtcgg acagcgcttc gattggatcg atcacaccat gcggataggc
     1501 cggtgcggaa ccgacgagaa acagcgtctg tggggtgatc gcttgggcca tggcggcgac
     1561 atcggcgcga aaatctgggc ccggcggcac cagcaccggc tttaccccca aatagtgtgc
     1621 ggacttgagc agcgccgggt gggctgtcgt cggcaacacc atttcgggcg cagtgatctg
     1681 gggccgttct ttgcgggctt gatcgcggta ggtcttcacc gccaaaaaca gactttcggt
     1741 gccacccgat gtcatcgaac cgacgacctg ctgatcgcca ccgagcagtc cgctgcacat
     1801 ggcgaccaca tcggtttcca tcttgcgcag actggggaac gccatcggat tgagcgcgtt
     1861 ttccgccaaa aacagcgaat aggcatcgcg cagaagctgc aagtgctcgt cggtgtcgtg
     1921 ataaaccaga ctgaacgtgc ggccatcacg ccagcgggca tcgcggctgc gcattgcttg
     1981 catttcggcg agcacgtcgc tggcgctgcg gctgtttaca ggcagggcgg ggcgtcctgg
     2041 ggcggtctgc ggactcgaac ttggtgggct ttgcggatcg gtttgcgaca tgggcgcagc
     2101 gtatcacgac cccaactttg ccgtctgacg atgcgcgcat gcgttggttt tgcggaccac
     2161 ttgccaggtc tacgcggtct tggctttggg cggtcccgct gtggtgatca gtgccacggc
     2221 aaaaatgacc atcagtccgg cgacgaacgt gcgtgagctg ggcgtttctc cgttataggc
     2281 ccagccgagc aggacggcga cgagcggatt cacatacgca taggacgtag cgacgatcgc
     2341 ggtggtgttg cgcaagagcc acacaaacgc ggtaaagcca atcaccgaac cgaacagcat
     2401 caagtagccc accgaaagaa tcgatttcag cgtgaccgcc tgcgggtcga tttgcgacca
     2461 ctcgccgcgc agactgccga gcaaaagcag cagcgaacct ccgcacagca tctgcatcgc
     2521 cgtcgacaga accggagagc tgggcagctt gacccgtttg gaaaacaccg atcctgctgc
     2581 ccaggatacc gacgccagca gcagcgccgc ggcacccgct gggtcaattg aacccgactt
     2641 ttgcggctgc accaggacaa acactcccac aagtccgcac aggatgccga cgatggcgcg
     2701 tggcgctggc cgggttccac caggtagcag cgtcgacagc accaccgccc acagcggcac
     2761 tgtcgccacc atcagcgacg ccaggcccga tggcaccgtc ttttctgccc agcacaccgc
     2821 accgttgccg cagaccagca tcagcacacc gatgagcgcg gctccctgcc attccgaccg
     2881 ggtaggcatc ggtgccgatg acaacttccc gacggtgaac ataaacaaac cagcggcgac
     2941 aaaacgcaga ccggccatcg tgaacggcgg cagtgagtcg atcgcaaagc gaatcgccag
     3001 atacgtcgag ccccagatca catacaccgc aaccagggcg aggatgattc gcaacgtgtg
     3061 cgcgcgaaat ttcatgggcg gcagacatac gcggccccgc ggcgaactgc aagattccag
     3121 cgtcgagtcc gtggtaggta aatttgcctg gtagcgcgga tctggcgctg gcccggtcgg
     3181 cgcaagggtg tgctgatggc ggcgcaattt gtgcctgtca tttcgccgcg gcttgacgga
     3241 aattttgtga ctaagcacac tgtttgttta gttcacgatg gcgagcgacg cggtggttct
     3301 cgacaagcgc tttcagctcg gcaaggtgct ggggcgcggg ccttccgggg tggttcatct
     3361 ggcaaccgac ctgagccgtg gcagcagctg cgtcgtcaag cgcatccacg ccaagtacag
     3421 caatcgcaac atcctggatc gggtccaagc tctcagccat gcggcggcgg acatgatgca
     3481 tccggtggtg ctgggggtgc tctataccgg gtatgaacca tcgggaacac tgtatttggt
     3541 atcaccgttt atcgatggtg agtcgctcaa ggctcggctg gcgcgcggac cgctttcact
     3601 tacggcgacg ctggcgatgt tggaaccgct ctgctcggcg ctgcaagcgg cggcggaacg
     3661 ggatctcgcg cacggcgcac tgcatccaag caatgtgttc tttccgacgg aaggcggcgt
     3721 gcttctgtcc gactttggca tggccgagct gcgggtgtcg gcgggggcca agtgggatgg
     3781 tccgctcagt tatgttgcgc ccgaatcgct ggaaacgggg gctgcgccgg caactgtgcg
     3841 cgccgacgtg ttttctctcg gggtgctcgt ctacgagtgt ctgatcggcc agccgatgtt
     3901 tgatgccaaa tccctggcgt cgtatctggc gatggtcggc acgccaccga agatgaccgt
     3961 gaagcatccg tcgtatgcgc acctcgacgc agtgctcgag atggcggcgc aggccgatcc
     4021 agaggcgcgg tttgcgacgg tgcaagccct gtggcgggcg ctccaatcgg cgctgctcga
     4081 ccttcccgcg agcttggcca acaacctgac acgcgggatg gcgccatcgg cgcagcgcac
     4141 cgccagcccg tcgaaaccgc cgcaggctcg caaaccggga tcgaaaccgt cggtgccgcc
     4201 gcgtccaccg tcgggcgcac cggccccacc agccacggct ccgcgacccg aaagcggatc
     4261 aaatcctccg gcgcgtcccg tccaggcgat gccacagctt ctgatcgaag aagaaatcga
     4321 agcgccggag ccgctggagc cgtcgggagc actggtcgca ccggctcggc cccagcccac
     4381 gaaagttccg cccaaagcgc agctcccggt gacgcagaag ctgccagagc ccctgccacc
     4441 accgcctgtg tccgccaaag cagcagcgac ggcacctgtc ggtccggtgc ccgtcgctgc
     4501 gccgaaaccc gccctggctg cggcccgacc tacggcagcc aaagccgcgc atcggcccgt
     4561 tgcggcgatg gccccgattc cgtttggtga tgatgcagcc ggtgcagcag cgacaggagt
     4621 gcgggcccct ggtccgcccc gtccgcagag gctcgatccg cagcagattt tgccgaccga
     4681 tccatcctat cgactgcgga ctcctccgcc atccaagctg ggggccatgg ctccgctgtt
     4741 ttgggcgacc atcggtggaa ttgtcgtcgg cggatcgatg attggcgtcc agtattattc
     4801 ccgtctgtcg gcgcgtccgc aggccgtgca cagtgcggcc acgacaatcg atatagaggg
     4861 actcttgcga caagcagaga cggagtctct ttcacgaaac tatgccagtg cgttcgcgta
     4921 cacggatctg attttgcgaa cccagccgtc gcacacggcg ggaaaagcgg tcgctgaaca
     4981 agccagtgaa cagcttcgtg tcagtgctgt ctacggtgcc tttttgcgcg ccgcggatcg
     5041 tgaacaatcc gatcttgcgg tggcacttta tcgcgagctg ccgcctggtt cccccttccg
     5101 ttctcaagcg tgggacccgt ttatgaaagt ccggaattcg tttgtccggc gcagactggc
     5161 ggtggcaacg gcagcgctga ccgcggcgga atgcgatgga atccgagaac agatcgaaaa
     5221 gctctacttc atttcggaca gtgcgaccga ctctgcactt cagcaggggc agcggctttt
     5281 gggaaagtgc aagagcgggc agggaaccga agcgatcgcg gaagcgcaca ttgagcgcgg
     5341 cgacaagaca gacaaaccgg aaaagacagc gaaagcggac aaagccgcca aagcggaaaa
     5401 aacagacaag cctgaacttg tggcggtgaa cgacgcgggc gaaacggaaa aaaagaagaa
     5461 gcggaaggcc aaagacaagc cttccacaga caagcccagc gacggtgcag caaagccaaa
     5521 ggaagaaccc aaagagagtc ttccgagcgc gctcaggaat ccgttttagt tgctctgggt
     5581 gtcgacttcg ttctgctgcg cacggcgaag tttgtagccg cgcttggcca gtccgccccg
     5641 tcttccgatg ttggccatgt agtcgcgatt ttcacttatc acggcgcccc cttttttacc
     5701 ggccaggctt gcttcttccg gattgaaacg gtgggcattc ccattggcgt gtgccgtctt
     5761 gccgccgagt cgggcaatct cacgccgcct ctctgcatcg agcaccgcaa agccacgtcg
     5821 aaccggtttc ttgacggcag gcgttgtgcc atcaagtgcc gcaggagaaa gcgccgccgg
     5881 ggaaagtgct gccggggaaa gtgctgccgg ggaaagtgct gccggggaaa gtgccgcagg
     5941 agaaagcgct gcttccaggg gagccagtgc tgccgaggtc gcgcttgtgc gtgcagtcgg
     6001 tttcgcttcg atcgtgttct gctcgtacat gatggactcc tttttgggtc tggttctggt
     6061 ccggaatgct gttgagtctc gtttggtaaa atcaaatgcc gtgcgctacc tggtgggaat
     6121 cacaacgtgg gcgcttggcg acgaaaactg gtaagcgttg ggaatccatt gcggagtctt
     6181 cctaaggcaa aaaagaagac tcctaaaagt tctctgcaga ctcccaaaat gaaaaggaat
     6241 tggagtatgt gttgtctgcg tttgctgcat tgcactgtcg agttcggatg cagcaatgac
     6301 accaagctag gacaacgaaa tgttggggaa tgcgcagaca tttttggaca atgaagattc
     6361 gctggcacaa tcccagccgg aagaatttcc tgcgctgatg gagccagatc cggtagcgga
     6421 atcgacggaa agagcccaga gagggctgct ggaagcgatg acggcactgg aggatcttcg
     6481 cgacttggca cctgggcaaa aaagggcact gcgctccttt ctcaaggagc tggggaccat
     6541 ctaccaagcg ctgggtggga agtcgcggac cccgcagcgg catgcccagt tggcgcggct
     6601 gcttcgcgat cgccggttgg ctctgggaat gagtctggaa gcgctggcaa aacgaatcgg
     6661 ggtgtctgca agcacgctca aacagatcga aagaggagtc cgtccgccga gtcgcaagac
     6721 actccttctt ttgcaaaatg cgatcgaact tgagctaaac ctcgacggga ttttggccaa
     6781 ggagtccagc gccaagccag gggcatcgct caactgctac atcgccccag gatatgagcc
     6841 ggtcaaaatg gtgatggaac tttccgagat gctaaacggt ccaggtggct atctggaaca
     6901 gaccagtgcg tatctggaac atcagagcgc gctccagttt ctgcggatgg ccaatgatcc
     6961 ggcgtatgtc tctgcattcc gggcgggcaa tccgcttgat cggatcgcga aagtgatttc
     7021 ggaaaacacc cgcggcatgg gaatcgagat gatcgcgctg ggggctggcg atgcgcgcga
     7081 ggaagtccgt ctggtgcaag acatcgtcgc caggatggaa attcctgatc ttcgcttgta
     7141 tctgctcgac atcagtcaac ccctgctcag cgaagggttt aagcatgctg cggacgtgct
     7201 gtgtagccag cagggcgtgt cgtattttgc gatgcaagcg aacttccatc acatggaacg
     7261 gtatccgcag ctgcactaca caccggaacg atcgcaccga cggaggctgg tgacactcct
     7321 gggccggacg attgggaatt tggacaacga acccaagttt ttcaaataca acttggtggg
     7381 ttgtcaggaa ggggatctgc tgctgatgga ctttcagctc gcgtatgctt cgacgagcga
     7441 tctggcggca attcgtcgac gggaaccggt gttatctggg ggtcgcgcgc aaggacagag
     7501 agcgcgtgag gactggctcg cgagtgcgat ttggcggcac tgtcgcgatg tcaccgatgt
     7561 gaagtttcac tatgatctac tgaccgactg ttctgtgccg ggaagttaca gcatcgaagc
     7621 cgtagcgacg gtacatgcac gcggaaggga tccgcggcgg ttctctctgt ttcgattccg
     7681 tcgttatgat ccggaccagc ttgcgagctg tctgagcggt ctgggatggg acttggtgga
     7741 agattttccg cgcccacaac cacaaggcgg aggcgactgt tggcttctgt tccgtcgggc
     7801 caaacagccg aaataggaga cgcttttccg acggggaagg gcgattgtta gcgctttttg
     7861 gccatcaacc gatcgttcat ttcaatgagc gctcggaatg cagaaggaag cgcgcgtttg
     7921 agccgccaca gccatcttcc atcggccatg ggaacgcaat aaagctgacc cgcttccacc
     7981 gcttgcagcg tctttttgac gacttcttcg acggggcgtc cgcgctcgac cagtttttcc
     8041 gccatccgtt tgatgcgcgg atcttcaaac attccatcgg cggcaatgtt ggtacggaag
     8101 aacgtcggac acaggaccgt gacgctgatg ttggtgccgg tcagctcccc ggccagcgtt
     8161 tcacttagcg aaaccacacc cgctttggcg acgttgtagg ctcccattcg cggtgcggcc
     8221 aaaagtcctg ctgccgatgc aacgttgagg atggctccgt gtccttgctg gcgcagcgcc
     8281 ggaacaaaga cgtggcagcc gtagatgacg ccccacagat cgatgtcgat ggtcttgcgc
     8341 caggtttcga tgggaatttc gccaaccaaa ccgccgctgc tgatgccggc gttgttgacg
     8401 accagatcga tgggggtgct gccggcggcg tctctcagcg cttgcacgtc ttcgagcttg
     8461 gacacatcgc agcgcacggt cgtcacggcc tgtgcccccg cttcgcgtgc gagcttggcg
     8521 gtttcttcga gcgccgaaag attgatgtcg ccgagcagca agcgtccgcc acgacggcca
     8581 agctcgatgg cgagagcgcg gccaaaaccg ctggcagcgc cggtgatgat gccaaacgtc
     8641 gaggcaccca atggtctttg tgtcatggct tctccgattg tggtccccaa acccagggga
     8701 cggtgcgtac ttcacggtag atggctattg aatcttggtc aatagtcgct atgctcaccg
     8761 gatggatcgc tctccctccc gtcgcaaagc cggggcccag gtggctgatg cggcggtgcg
     8821 caagccgacg accagccgcg cggcgaaacg accgcagcgg gcccggttgc tcgtcgatga
     8881 acggcgtgcg cagcttttgg cgctgggcat caagctgttt gccgagcggt cctacgacga
     8941 agtgtcgatc gatgagctgg cgcgggcggc gaacatttcc aaagggctgc tctatcacta
     9001 ttttccgacg aagcgagacc tttacacggc ggcgcttcgg caggctgcgc agcagcttct
     9061 cgacgaaacg cagccgcttg cgtgtttgcc gcaggacgag cgcgcccgtc gcgggctgga
     9121 gacgtatctg gcgtttgccg accggcacgg accagcctat gtcgcgctga tgcgcggggg
     9181 actgggcgcc gaccgcgaga ttgtcggtat tttggaagcg acgcggggca cgatagcgca
     9241 gcgaattttg gatgcgttgc cgacggggat tgcaacgccc ctcatccgcg cggcggtgcg
     9301 tggctggatc ggttttgtcg aagcgacggc gctcgactgg ctggcccaca aacaggtgga
     9361 tcgcgacaga ctcgtcgagc tactgctcgg gcttttgttt gacgccatcg cgcgggccgc
     9421 ggtcgcaggg taagcgcggc tgcacccgac cggcggtgag cagatggcgt ccggcacttt
     9481 tctgcgtcac gtcgatgagc ccggcgtgca aaagcgtcgc cgacagcagt tcccgcggaa
     9541 gctgaccgga tcgactgcac agcaccacca ttccgtcgct gcgcacggtt gccaaaagcg
     9601 ccgtcaagtc atctagttgg gcacgctgtt tcaaaagcac acacagcgca tcgacggcaa
     9661 ttccgacggg actttgcgcc gcactctgcg cgaccaccac ggagcagccg gcagctttga
     9721 gcggtttgct cagtttttca tcgctggtga gcacgcgaag cggccatagc tggccttgtc
     9781 cgtcggaaaa acgcaagtca tgcagaacgg tcgatagtgc cgaggcgagc gctcctggcc
     9841 ggatgcgctg ccacagtcga ctgattcctt ggcttcctgc gtcgcggatc gactcaaaca
     9901 tcggcggctt tatatcaaac gcgcacgatg cgtgcgagga accagatacc cagcacaagc
     9961 ccaagtccga ccaccagccg tcgcagtcgc gtcggatcga tccgctgcat gacccgcgcc
    10021 gtcagatatc cgccaatcac cgatcccgcc gatacataga gcgccgaccc cagatcgacc
    10081 aatccctggc gcagcaaaag cagcgatgcc aaaaggttga taagcagtcc cagccaggcc
    10141 tttacggcgt tgtgtgcatg gatgtctccg tcgacaaaca ggccgaacag cgccagcatc
    10201 aagatgccca tccccgcgcc gaaatagccg ccatacgtcg cgataagaaa ctggagaaag
    10261 agacccagcc acgtcggaat ctgccgccgc tgcttttgcc acgcgcgtat cgtcggttgc
    10321 agggccaaaa gacccgtcgc cagcaaaatc aatggcggca ccgcccagcc gaacactcgt
    10381 tcgcccccca gggtcagaat ctgggccccg agaagtgcgc ccattaccgt cggtaacagc
    10441 agcttgggga tctgatgctt ggcggcagcc agcggtccaa aaagtcccag cgcgctgccc
    10501 agactgcccg gccacagcga cgcggaattg gttgcattgg cgacaagcgg cgaacagccg
    10561 agcgcgacca gcgtcggaaa cgtcaccagg gaaccaccac cgcccacggt gtttagcccc
    10621 gacgcaaccg ctccggccaa aaacaaaagc agtccgcgcg tgagcgggtg aaaaaacgca
    10681 tccgtcgaca tcgtcgcggc taatttcccc gtctcagcac ccgacgcagg atcttgccgc
    10741 gatccgacct tggcagatcg ctcacgaact cgacgcgact tggggctttg tagtgcgcca
    10801 gcttttgttt gcagtgctgg atcagtgcat cggccaagct gtcgtcggcg ctgtggccgc
    10861 tgcgaatcac gacataggcc agcgcgcgga ccagaccgtg ctgattttct tcgccgacaa
    10921 ccgcgcattc gacgacggct ggatgacccg tcaggacgtt ttcgacttcg agcggcgaca
    10981 cgaaaatgcc gccaaccttg agcagatcat cggcccgtcc gtcgtaaaag aagaaaccgt
    11041 cttcgtcgcg gtggaacaga tcgccggata ccacccagtc gccgcgcagc gtggttttgc
    11101 tctgctcgtg cgcttggaaa tagcacagtg ccgccgagtc accttcgacc cacaacgtgc
    11161 caatttcccc tggttctgcg tcgctgccgt cgggccgaac gatccgcgct gcgtatcccg
    11221 gaacaagctg cccgagtgaa ccggggcgca cgtttcctgg ctggttcgag atgtagatgt
    11281 gaaacatctc tgccgagcca atgccgtcga ggatttcgac gccataggtg cgaaaaaaac
    11341 gatcggccag ctccgcaggc agcgcctcgc ccgccgatgt gatgagccgg agcgacgaca
    11401 gcttggcatc tggatcgtga tcaagcagtc gctgaagcgc agttggcacc gacgccagca
    11461 ccgtgacttg gtggcgactt atcctatcga gcagcacttc tggcgtcgga cgaccgcgga
    11521 acagcacaca gcttccaccg acggcaaacg gaaacatcag attggttccc gtcgcatagc
    11581 caaaatacag ccgcgacacc gccgtcgtgc gatcggctgc cgacagcccc agcacgtttt
    11641 tggcgtaaca ctcggtgttg tagggaaagt cgtggtgaaa gtggacggct gctttgggtt
    11701 ttcccgtcga tccactggtg aaaagccaga ccgccggatc gtctttgctg gtgggatagg
    11761 tgccgcggcg cgggctgctt tggaccaagt cccacgtcaa gacttgaatg tgtgacggaa
    11821 tgcgcgccgc cgcgagcaga tccatgtcac agtcaccgac gatgagcacc gctcgcaaac
    11881 agtcgcattc gggcagaagc ggtgcgatgc ggatcaccaa atcggcactc gcaatcagtg
    11941 cccgacagcg ggtgtagcgc agatagtaaa ggtagtcttc cgtcggaaga tccgggttta
    12001 ccatcgcgac gacagcgccc gctttcaaga tgccgaagaa gctttcgaca aactccacgc
    12061 agtcgggaag cccgatgaga cagcgatcct cggggcggat gtcggcttgc cagagcgcgc
    12121 cacagacttg ttccgacctt tcgacgatct gcgcataggt gtaactaccg tggtcgtcgt
    12181 agaccgccac tttgtcgccg cgaccttcac ggatgcgctg gtcgagaaag tagtcggcca
    12241 tgttgaaggt ctcgggaaac gtcgggcgtt ttggcaaacc ttggttcatg cgtggagcat
    12301 aacaaacccc acccaccgga cctgtggttc atcgtacaga tgtgacaact gtgcgacggg
    12361 ggtttccgcc acaggctcgg gctgttacga tgtgttagcg cgagtttgtc ttcgaggact
    12421 ggtttggggc gaaacactgt ccgggcgcta caccgtcgat tgctttgctc gtggctgcgc
    12481 tacggctggc tgctcgtgct gcttggtttg ccgcgactgg ccgcggcgca gtctttgggg
    12541 caagccgagc tgcggatggg ggagccggcc ccgcgtggtc agcagtggtc gccgctgcgt
    12601 gaactggcga agctggagta tccggggctg tgggaataca actggggcga ctcgccgcgc
    12661 gacgcgctgg gtcggctggt ctattcgatt cccgaaacac cgacgcgcga ctggcaaccg
    12721 ctgggccgct atgagctgcc caaaggccga aatggtcgtc gctggctgtg gctgcgatcg
    12781 gaaattccgg cctgggcatc gacgctcaaa gaccccgtgt tgcagatgcc gcatctggac
    12841 caagaattcc aagtcttcgt cgagggaaag ctggtctacg agtggggcga tttttcgacc
    12901 gataagccgt cgtatctggg ctatcgcggg cacatcgtcc gtctgccgcc cggctgtggc
    12961 ggcaagcgcc tgacgctgcg catctattcc gaccacgtca acattggccc cgccggcccg
    13021 atgcgctttg gtgatcacgg cgaaatcctg gtcaatctgt tcaagaccga tttgccgcga
    13081 cttatcgtcg gtgggctctt gtttttgctg gcgacgatgg gctttgcgtt gttttcgttt
    13141 cggcgcgagg aaaaggtctt tttgacctac ggtttcttgg cgctcagcgg cgggatttac
    13201 attttggcgc aggcgcaagt ccggctgttt ttgttcgacc acccgctcct gctgactcac
    13261 atcgagctgt ggtcgctgca aatcacgatg ctggcgctgt gtcacttcgt cgagtcgatg
    13321 ttcggttctg gcccgctgcg cattctttac tatctgcgac agcttcagtg ggcgtacctg
    13381 tttggctcgg cgctggccgt cgcgctgggg cttgtgccgg tgctcaaaac gctcttgccg
    13441 gtgcagctta tgttgtttgg cgacgcggcg ctctcggtgg cgctgccgct gtactattcg
    13501 tttcgcggca acatcgaagc gaggatcttt gcgacgggat ttctgggcgc gggggcgatt
    13561 gccgtctatg acgtgctggt tgctctcggg gttttgtcgc ggacccagcc gaagcttgga
    13621 cacatcggct atgcgctgtt ttttgtgacg ctgggggtga tcctggcgcg gcgggttctg
    13681 cgggcgcatc agcggctcca gaactacacg tcgattttga acctgagctt ggcttcgtcg
    13741 cgggtcttgg ttcccgacga gcgagcgcaa gtcgcgctat cggaactggt tcggcttctt
    13801 tcggcgcgac gggcgtttct gtttttgtgg aacgaacaaa gcgggcagct cgagtttgcg
    13861 acggggcgca gcaacgatgg gcagagcctg ggggctgcgg aaggctgcga cttggggttt
    13921 gtcgagcagt cgatgacggc ccaaaaaccg gctgcacggc ggtcggcgga caagaaagtc
    13981 gtccagatgg tggcgccgct cgaagcccgt ggtcaaaagc tcggcgtgct gtacatcgaa
    14041 agcgccagca cgcggcaggg attgcacgaa gaagacctcg acgtgctgat tggactggcc
    14101 caccaagtcg cgatcagcat tctgtcggcg cgcacgctgc gaattgaagt cgacgctgcg
    14161 atggcgcaaa agcgcatgca agaacagcag attttgctcg acgcagcggt tcggatggcg
    14221 gcaggtgacc tgagcacgcc ggtcacggtg ccgagcggca gcacgatgac ccatcttgcc
    14281 gaggcgctgg aggcgatgcg caccgatctg ctgaccaaag ttctcgaaat ggagagcaaa
    14341 ggtcgagaag tgcagacact gaacgaggag ctgcgacgac aaattgccga acgctcgcgc
    14401 cggttgatgt cggtcatcat tcgcagcaaa gccggcaaga gcacgccgct gcccgatctt
    14461 tcgccgggca aagtcgtagt cggacgctat cgggtcgtgc gcaaaatcgg cgaaggcgcg
    14521 atgggcgtgg ttcacgaggt cgagcgaatc agcgacggaa gacacctggc tgccaagttc
    14581 gtgccgcagc tgtccgacaa aaaatcgctg ttgcgtttca tgcgcgaagc ccaaatcttg
    14641 gcgcggctgc gtcaccccaa cttgatcggc atcaccgacg tcgacatgac cgctggcggc
    14701 atcctgttta tgatcatgga cttggtcgtc ggcggctcgc tcaagtcagc ccgtcgtcgc
    14761 ttcggcgaag tccgctgggc actctgcgtc ctccgccaaa tcgccgaagc cctagccgcc
    14821 atccacaccg ccggaatcgt ccaccgagac ctgaagccgg acaatgtttt gttggcgggg
    14881 ggggaggagt cggcgttgcc gtttgtgaag ctggcggact ttggggtgtc gcttttgatg
    14941 tcgaaagtgc agacctcgcc gctgtctcgg gcgctgacgc agccgattca ggcggtggtg
    15001 gacccgctgg aggaaagcac ggaaatgcag gtgccggggc ccgattggtt cggcaccgag
    15061 gaccgcgacg tggacttggg tgaggatggc ttcgcggata cagcggacat tccggtgcaa
    15121 ccggccagga cgacgggcgg cgtgctggtt gtgagcggtc agccgagcgc ggaggaaggc
    15181 gcgctgacac tgcaactgcc gcctggcgga cgcacgacgg gcccagtggg agcgccgcgg
    15241 gaatcgacgg gaagtgcggc acggaagaac tcctcgcctg attcgctcgc ggccactggt
    15301 gaatcgtcgg tgtcgggcgt gcaaggtccg gtgacgcagt cgggtatttt gattggtacg
    15361 ccgatgtaca tggcgccaga gctgtggcat tccggctcgc atcgggcgca gccgtcgtcg
    15421 gatctgttca gctttggggt gattgcgttc gagcttctga cgagcgtgct gccgttcctg
    15481 cagccgccag tgtttgccta tgcccagaag cagacgctgc ggattgccaa gctggcaacg
    15541 tcgcggtccg agctgccgcc gaagctgtgt gcgctggtcg attcgtgtct gtctgaaaac
    15601 ccggcggcgc ggccctcggc gcgggtggtt gtggaacagc tgcgagagct gggggcaccg
    15661 acgccgatgt cgtcgagcgg ttagccagtg agccagttcc aaacgcgacg tgcgccgccc
    15721 gccaccgcac tgccggcagc acgagcgccg cttgcgacgg ctgatgcgcc gtttgcgacg
    15781 gcaccgccga cggctgatgc accgtttgcg atggctccac cgacggctga agcgccgttt
    15841 gcgatggctc ggcccgcggt gcccgccgca tttgcgacgc cgttgacggc agcgccaccg
    15901 agtgaacggg ctccatccca ggcttgaccc gcgaggtggg cagcccccgc cccagcgacg
    15961 ttcaagaaac cgcctgtggc accgagagcg ccctcccagc caagtccacg gatgttgttg
    16021 tacgcgcccg agacgccttg ccacgcttcg cgtccggtgt tggccagcca gttcccggcg
    16081 ccgcgaattc cctgaccgat gccgctcgca gcgtcgccca gtgagttcca gttgcgcgcc
    16141 ccgttgacaa agttaccaac gcccgacgca gcttcctggg cgcggttgcc tgcccaatcc
    16201 acggcatctt gacccatgac tcgcgcgccg aaaaagcctt gcaggccgcg gccaaagctc
    16261 tgggcgtcgg cggaatagcg gttgccattt tgggccattt ccgcgcgggt atcggccacc
    16321 aggcgacggt cgtcttctcg ggtcgcttgc agaccttgcc aagtgaacat gtcggcagca
    16381 cccgaatccg cccccaggtg ctggtagtac ccgtgatcgt gacgctgcgc cgcggcatcc
    16441 atcccgtcga ccgcccgata gcgcgggttg tttagcatgt gcggatcgac ataaggatcg
    16501 tagtttggat tttgcttgcg ctgctgagcg atgtagtcgg cctcttcgtg atccatgcgg
    16561 ttgccgggtc cggcccagtt gccgtagccg acgaagcgct cgacgggatt ggttcgccac
    16621 gaatgctcag aatcgccttg cgtgtggtgg ccatcgtaag gcgtagcttc gtagtccgac
    16681 atctgatcct ccccaggtga tggtccgtgt tgcgttcgcg taagcagcga cggtcacagc
    16741 ttgccatcca gattatcggc agacaggcag aaacgttgtg atgtctcccg tcggggctgt
    16801 agtacactta gcggctggag ttttcatgtc aggacgcagg ttgtggattg gtcttgccgt
    16861 cggggctgtt ggtctgggat cgcttttgtg ggcgcgatcg aaagacacgg cgcagtcgtc
    16921 ttcggccact tcaggagaaa aagccgcgcc caattcttcc gacggtgccg ccaaaaccgg
    16981 ctggacgcgg gcgcatcgac tggcgacgca gctggaccat cccaaaggcg tggttgtcga
    17041 tggggagttt gcctatgtcg tgaccggcgg atttgcccag gccataaacg cggtgttgcg
    17101 gatttcggtg acatcgggcg ccgtcgaaac actcgtcaaa gttccgcaga ttttgtcggg
    17161 cgagctcgcc atcgacgaca aatatgtcta cttttcgagc gaagccgaca atcgcatctt
    17221 tcgcgtcagt cggattggtg ggcctgccgt cacagtttgt gaaacgatca ggccgatcca
    17281 cctcgccgtc gatgaaaccc atgtctattt tgcttccctc aagtcggaaa ctgccgacgg
    17341 cgctgtcttg aaaatcagca aactaggcgg tcagccgcag gtgctcgtcg caggtcagac
    17401 gggcgtggat cagctcgcgg tggatcgtga cgcgctgtat ttccgcagca acagcggact
    17461 gtggcgcgtc ggcaagcagg gcggggcggc ggactgtctt ttgccggcag cacggggaaa
    17521 aaacgtcgat cacttggtcg ccgacgaaac ccacttgtac tttttctggg agacgagcgg
    17581 caacggcaag tatcgcgtcg cgcgaatgcc caagcgaggc ggcgcacccg aggcgattgg
    17641 cccgattgcc aacccgacgg gacggctcgc gctgtcggac agccacgtct attttttccg
    17701 cgccgcagac ctgaccgacg atgcgcttgc caaggttgaa aagcagggcg gtgagcctgc
    17761 attggtcgac ggcagcggct acagcaccgg ttatctcacg gtatcgctcg gcgatgtcta
    17821 tttcaccgat gtaaacagcc tatatcgcgt tcccaagtag ttcgtgggcc gtcacgtctt
    17881 ccttctgcgc acagacgagc gggagcttga gggcaagctc ggtggtcagg tccattgctt
    17941 cgtccttttc accctgccga tccgtcgtca cctgacaggc aagaccgacc gacgccgctt
    18001 ggctgtcgaa cagcgacaga ctcagcgtgt ccagcttggc cccgctgtct gccaaccgca
    18061 aaactacact cggggtatcg acgggcatcc gcagcagttc gatgtcgatc tgggctcgct
    18121 gctcgggcag aagccgcggc actgtccgca aaagccaacg cgaaatcaga agcgaaagat
    18181 cgctgcgcgt cgcttgcact gtgatggccg gctgctgtct gggcggcttg cgcagatcaa
    18241 acgtctgagt cgcaccgcca tcgcgcagtc gcactgtcag cggaacgcgc tcggcaaaca
    18301 actgatacag atcgaacagt agaggcagaa gtggcaccgt ggtctgcgtc gtcgtcccac
    18361 tgctgacgta gccgcgcatc gcacgcacca cgtcgtgggc ccgtcgaccg cctcgctgca
    18421 agagatcgac catttggcgc aactcggtca gcatcgccag cgcttccgtg cgcacgtcgt
    18481 cggcgatggt gtcatcttgt gccgtggtca gcagtttgtc gccaagctcg tgatgggttt
    18541 cgcaaagtgg ttccaagagc gcgaccatgc tgccgaccgg attgttgatc tccgacgtga
    18601 gacttgcgac aaccgatcca atcgccgcga gccgttcgcg gtggagcagc tcgtctcggg
    18661 cccgtccaag tgacaccagc gcttgctgca gttcgctgtt gcggcgggcc agatccgcat
    18721 tgcggcgcgc gacctcgcct tccaactgaa gattgagttc ggtactggat cgcaggcggt
    18781 cgaggagcgc tcgccgcaga ccttccagtc gcaaaagcac cgtccacagc tcgccgggca
    18841 gcggttcact cagcaacagg cgattccatt ccccgcggga cagcgccagg acctgctcgc
    18901 gcagccgcaa aagcggacgg acccagcccg accagacgag cagtgccagc cagccagcca
    18961 gcgcgaaagt gactccgagg acgagctgcc acatcagcga gagccataca ctgtccttgc
    19021 gcccccgtga atgaaaaaac actgcgcttt cgccgcccat tcggtagcgc actcagccag
    19081 ttcggttaat atagggcaca tgaaatccac actcttttct gcgtgtgctc tcgtcggagc
    19141 aatggcgatt ccggctgccg cttctgcgca aaactgcgcg gttcattttg ccgcaggttt
    19201 cggaagcgtc tcgggatcgg tagccaaggc cacgacccag cccgctgccg acgtgctggt
    19261 ggaccaggcg tttggtccgc gtccacagca ctgcgaagcc ggtgcctaca cctcattcct
    19321 tgaacagttt gaaaacttcg ggcggacggc gatccgcacc gcgcaaccga cgcgcgggag
    19381 caaagcaccg attgcgccgg tgaaggaaaa ccagctgcgg ctcgcgattt cgatcgtaaa
    19441 caagatgtcg ccgcagaaag tgccgctcgc cgattccaag accgcgccca gccagtggcg
    19501 tcaggtgcgc agcaatctca acgcggtggc tgatgatgcg ggcaacacgc cgatgatggt
    19561 gcagtttctg gacgcagtgg gccgcatcag tccgcccggc ccgatcgaag aagtcgtgac
    19621 tgtggggcag ggggctccct ccggatcgtc ggttgctacg ccgggcatca gcgcgcctcc
    19681 gggtggcgtg cagcaagttc gggtgccgct ggttccgctt ccctcgtggg ccgtcatcaa
    19741 gctgtacgaa gcgcgcgacc tgtgcaaacc gcaggatatc gcgggcattc agctccgttt
    19801 gcaagacgtg atcaactgga tggaatcaaa cacccagccg ccgcagtagt ccgtcgttct
    19861 gctcgacgct cgctccgcag tccttttcct tcccgcagac cgcctttttc cgcagcgccc
    19921 cgcgtgttct atggtccgtt ttggtattcc aaaactggaa accaggttgc gtatttaggc
    19981 attctgtacg tttcatttca cgtttgaccg cgccgaaact gcgaactacg atcgagggat
    20041 aacccatcat ttggattact cctccatgaa cacgtacagg cagatccgcg caaacacgct
    20101 atctggtttt ggtttttcct tggtcatggg aattgcttcc ctggtcggct gttcgggggc
    20161 gaccgacacg acgccgacac cgacgccagc tccgcccgat atggcgcttc agtacagtta
    20221 cacgccggca acgatcaatc agatcgatac cgatccggga aatggtccct ttgggaatgc
    20281 ggtccgcgtg gtgcttgatc gcgtcgtggc ggtgtccaaa gtggacaaat acgtaaactc
    20341 cgcgaaccag cagtgtcgct accaaatttg ggtgcaagat ccagcctgta cggtgccacc
    20401 gtgcggtatg gtcgtaaagg caattggacc gatggctccc agtgctacct cgacgggaaa
    20461 agactgtccg gcagccagcg cgagtgggac tctgcttgat acgatcagcc gcggcgacaa
    20521 cgtccgcgtg cgtggcaaga tcgtggtgga agtcgacagc acgacaccga tgaccgtggt
    20581 cgaacatcag ctgtttgtcg aaacgctcga catcttgaca ccggacaagt cgataaaccc
    20641 ggttgtgatc acagacccgc tcgtctttgc gcagtttgtc acccacaaag gaacgacctg
    20701 gaacaagtat gaaggcatgt cggtgacgct ccagccgacg acaggaaagc ttcagattgc
    20761 caccatcaac ggtggcggat ttaccaccac gccgggtaac accgactggg gcaatacctt
    20821 cgattccgat tactttccgt ccggttccac cgcattccca accgttggca gtacgtacac
    20881 ctcgatctct ggtgtggtct cgactcgtca cggcggggaa atcatgccgg gcaggaacaa
    20941 agacttcgtt ccgtagtgaa gcgggccgat tcccaagcga ttccgaaact actgggacgg
    21001 tttcttttgc ggaaccgccg cttgggtcgt cttgggaatc ggaatgtgca gccacaaagg
    21061 cagcttgcga tcgttgtcaa agacgatcac gatgtgcttc ttgtcgtcgc tgagcgagat
    21121 tccttcgggc tttagtccca aaaacctgcg cagcatcacc acagatccat cgggcttccg
    21181 ccaccacagc gcaccacccc catcgggcgg tcgttttttc ggactgttgc ccaggatcaa
    21241 aagcgaacca tcgggcaaaa atcccatgtc cgaaattcct tccgttacgg tgccaccacc
    21301 tgccgcggct tccacagcga gcggaatgtc ggcaaacttg ctgatgcgac ctgctgaaat
    21361 cgcaccgtct ttcatcgccg aaagtacatc gcgcactttg agcacaatcg cggcgcctga
    21421 cgcagtctga ggcgctttca agccgacgta cagctcgccg ccgtgatagg acagcgcttc
    21481 gatatcgacg gaatcggaag gaatcacgcc cgcttcgacg atggctcctg ccagatcaaa
    21541 agatcccagg attttcaggg tacgtcccac cagcgtgagc tggaacagcc gacgccgtgc
    21601 agctttgacg tgtccctttt tgttttccga gtgcgaagtg gccagaaaca gtgtgtcatc
    21661 cggaccggaa cagagagcct ctgcatcgtt gagtttttcg attcctgcaa tcggaatcgg
    21721 cgtctcatcg aagactcctt cgcggctgag tgcaaagatc cacggcgcgt gattggtgcc
    21781 tttttctttg agcccggtat cgtcgctgac gacaagataa cgggaaagtg tcggattcca
    21841 caccacaccc gaagcttccg gcatactggt gatggccagc agcgattccg gaacgcgcag
    21901 cggcgtcgga tcatgtccca gcgccagcgc cgccagcaaa atcctagcga ggagtgacac
    21961 ggaaggcctc gcctggtagt agttccatcg gcttgtcgac tttgaccacg atccgtcttc
    22021 cccacgctgg tactgatgga gtcggacgtg aacggatcgg aacttcttct atcagcggtc
    22081 caagttccac caccgttccg taatgcggcg tcacgaccac gccggttttg cgcaggacga
    22141 tggcacggcc cagcggaaag tttgtcacct gtcgttctgg aacataggcc acaacatgtc
    22201 ctgggcgcgt ggtcacaacg gtcagaatcg gcgttccggc tcccacgaca tcgcctgggc
    22261 gaagcacaat ggttcccacc gttccatcga ccggagactt gagtaccaaa tccgcgagct
    22321 gctgatccag ctccgcaagc tctgcttctt tgacagacac cgcattgcgg aagggtccga
    22381 gtcgctcttc caagcgacga tcttgatccg tcttgggccg cggacgcagt cccatcagtt
    22441 cctgatctcg cacggtgccc agcggtcggg acgacaaatc ggcggacagc gactttttct
    22501 gtcgctgtgc cgcttcgact tccgcggcgc gcacaagctg atcccgcttc aaggtattca
    22561 gccgcttgag ctgcccagag tattcgcgca gctcggctcg catcctctgc acatcggcgt
    22621 aggcgcgcac cgcttggatc tgaccgcgca tcaaaaacga agcttggaca tcctgctcgg
    22681 cgaccaagtt ggctttcacc tggtccagct ctgccacgac gcgcgttcgc ttgagctgca
    22741 agtttttgtc gtccaggcgc gccagcacat cgcctgcttt gactttttgg cccagaacga
    22801 ccgtgacttc gcgaagtcgc gcggtctgta gcggcgcgat ctgatggatc acttcctctg
    22861 catagccaat ggttgtccct ggaccgacgg tgaatccgtg tgacaaatac caaaagcctg
    22921 ccagcgccaa agcgatgagc agccacttcg gaatcctgcc tatcaggttc cgtatggatt
    22981 ccttaaggct caatcgttgt gtcctgcata aggagagtgg ctccgcttat gcccggaatt
    23041 tcgccaatgg actgcaagat cagtgccttt tccgacgcat cacgcaggcg caggtgatag
    23101 gagtgctctt gcgttcctcc tcccaagtca cgcgcgttga taagtacaaa gctgggcgca
    23161 tatttttgca gcaccttgct tagcgttttc tgctggtttt cccccgtcgc cacttgcaag
    23221 cgcagcacgg cgttccacat ccggcgcgta ccaaagtctg aaaacgacag atacgccatc
    23281 gctgcacaaa acaccgcggt cccgagcatc gcgactgcaa atgccagcac gccacacgcg
    23341 acgccaacgc tcagtgcgac aaatgcgaac atcaagtcgc gggtgtcttt gagcgtcgaa
    23401 cggaaacgaa tcacggtgag cgcgccgaca agtccaattc cgcgcgcgac atcatcacca
    23461 atcgccagca tcaccagcgc tgacaccaca cccgctatcg ccagcgtgtt ggcaaagctc
    23521 cgcatgtacg aaagaccgga atacgtggcg acgtagaccc atgaaatggt catcgacagg
    23581 gcaaatgcca aaagcagcgc cgccagcgtg atgtcccagc cgatgcgcgt atccatgtca
    23641 gactctttct ccctgcggtg ggggggccgc ctgcgcgaga tccggcccga tcaagccgtc
    23701 accgcccagc gcttccaggc tcatcgccat cacgtacttg gagcacgccg atagctgaag
    23761 ctgcagccgc tgcaccatgt cggacaccca gcgtggcgca atgtttttga acttcagctc
    23821 tgccagcgtc ggtgcttccg gctgatagtt catctgtccg cacaagtgaa cccaggcttt
    23881 cggatccccc tgaagcgttg gtccgcgcgc cggttggtag cagatgtcgc ggtcaaatgt
    23941 gagccgaacg ccctccatcg aatcaatcga cgtaaaggcc tcgcgcgagc aggtgatcag
    24001 cactttgggc gacgccttga acacaatcat caggtacaga aagtgttcga gcgtcttcat
    24061 ctctgccgga ttcttcagct tgagaccttt gggaatttcg ccccgcagca gcgctggcac
    24121 tgcatccatc ggcaccacgg cgcgatcttt gaacgtcacc cgcttgatct tccgcttcac
    24181 ttcgaaatag gcattccccg acggtggatt tccataggca cggatgcgta gcttgggccg
    24241 gtcgggggtt ttttggacat gcagatccaa aaaatccagg tcgtccgaat ccagataaag
    24301 cgacgtgttg cgctggccac ccagagcaaa gtcatcgcag cgcagatgcg gtgccgtcag
    24361 cgtgcgcatt gcttgcactt gcgcatcggt cagccagtac ttgtactcaa agcgttcaaa
    24421 ggagatggga gcggctttca tgaccgactc tcctctagtg gttgtcccac cgccgcttgg
    24481 agcgccagcc acgcgatccg gaacgcgagc cgcgcatcga agtaatctcg ctgtgcgcgc
    24541 agtgcggttt cttcgcccgc aaggagtgtg accagatcaa tctgtccacc gcgcaaagac
    24601 agctccaaaa gctgttcgtg ttccgccaag atcggcagca cttcgcgctc ataacgcaaa
    24661 agcacactgc gccggacttg cagcgctgcg taggcgttaa acaccgactg tttcaggatc
    24721 agaaactgcg cttccgcaag cccgagcgcg agatcaacct gcgccgctgc ttcctggatc
    24781 ggcccttgat tttggttgag gacgggcagc ggaatttcga ttcctagcag cacttcgtcc
    24841 ggatagttcg tcgacgtggt gtgccgatag cgcgcgctga tttcaaacca tggccaggcc
    24901 tttgcacgtt cggcccgcag cgcatgttga cgctggatga tcttcgagtg tgcggctttg
    24961 agcgccggtc gggccgccag cgcttgttcg gccagggcat cggcagaagg aaggctcgcg
    25021 tgttccaaat cgactggaaa tgcgccgcgc agcttgaccg gggatgcggt cacttccccg
    25081 atttgggtgc gcagatcgga ctgtgcatcg acctggcgca gaagcagtgc atcgcgatcg
    25141 cgctcaatcg ccaacagcga gagctgcact tgattgagct cgatccgcgt cgccccgccg
    25201 tgcgaaagcc gggtcttgta aagctccagc acgcgcttgc gcaggccgat catctgctcg
    25261 cacaccgcga gctgtttttt cagcatcagc aaaagcgaat acgtgctgcg gacttggttt
    25321 tggataagcc actcttgctc ggcgatctcg tgatcgacct gttccagatg cgcgcgcgca
    25381 agtgatcgat tggccgcgta gacgataggc tgtggcggag cccacttcag cgtcgctgcc
    25441 gcgcccatgt ccgccccagt ttgcacatgg acgagctgaa gctgaaacgc cggattggta
    25501 atcatcgaag cggtcaccac ttgtccttcc gccaccccgc gctgtcgccg aaatgcacgc
    25561 aagttgggat tgatccgaag tgcccgcagc accgcttcgt cttccgtcag tccgtccgct
    25621 tcggcttcca ccttcgcgct ttggacctct tggctctttg tcaaggggct ttgcgcgtcg
    25681 ctctgtgtct gtggttccgc catcgccggt tttttggccg ctttcggggc cgcaaaaacc
    25741 tgcgcgcagc agatcaaaac agaaacgaag acaacgccgc gccacatggt gaacctacat
    25801 gctcagaatg gcccctgctc ccctgggact tggagtggcc accgactgag actcagtatt
    25861 cacattatac aggtaacaaa acggtgacat ctagacggtt cttacgagag agaaccgaat
    25921 ctggcgtctg attgcatggc ggcgatgtga ttttgcgcgc cggtgttctt acatcggacg
    25981 cagacgaccg gttgggatga tctgcttgta cacagatgcg ctctgccaga gcagcttggc
    26041 cgcggcgttt ccggtgccct caaagtactc gagcgtgacg gtcacgggcg ttcccgcggt
    26101 cagcgcgatg gtgccgcttg tttcgacggt gcctgtctgg gtcgcccagt tgtttatgac
    26161 ctgcacgccg tttacccaca gccggacgcc gtcatctgct tgcgtgaaga acgtgtacgt
    26221 ttcgctgtaa cgtggcacca ctttgccggt ccagcggatc tggaagttgt cggcaggaac
    26281 cagcggatcg ggagtgccac tggtccagtc gtagttaacc gtcggtgtaa ctttcgatgt
    26341 cttcagtgtt gctttggcaa aatcgcgcgt cgtgtcacct gccgccgtct ggtacatgtc
    26401 tgctttcaga ccgcaggttg tcgccgtaca cgtcgcttcg tcgccgaaat cgagcacgtc
    26461 gttgtgacgg taatcgccaa acgtctgcat gccttgtagc tcggtgtaga agttgcaaat
    26521 gctcgctgct gccggggacg ccacgtagtc cgaaatgcta aacgtcgtcg ctgcttgcag
    26581 cactttgctc ttccacgacg ccggattcaa aacgcccgtg gcatccagtt ccaaaagacg
    26641 cgcccgcagc tcgtttcgga acaccttgat aaacgcatct ttccagcggt tgtactggcc
    26701 gtttccttcc tcgccccaga agaacgaccg acccccttgc cagcctagcg cttcgcccca
    26761 ctcgcggtct ttgtcctgcg gaatcagaag ccactttccg tcggaagcac gctgatacag
    26821 aaagtgattg tggaagcctt catcccacgg ctcggcccag ttgcggatcg cgatgtaatc
    26881 gatcatcttc tgggtgtcga agtttgcgac caaaaaggct ttcagtgccg tgtaatcccc
    26941 cgacgccacc gccgctgcgc gcagcgtatt gagcgtttcg atggtggtct gcagcttctt
    27001 gttgttgtcc cagttgtatg tcttgcgatc gtaggtgtac ttgtagcgat catcgatcgt
    27061 ccactgggga caggtggtgt taagtcccag cgtcgtttca tcggaaacgc cccacggacc
    27121 ttccactgca cccgcgttgc cgtcggcttt gaacaggtcg cccacgcgct cacctggcgc
    27181 acgatatcgc ttcatcatgt cgccatcgat gtgttcgatg tcgagcatgt agtggtaata
    27241 cccgccgttt aggttcaacc ggacaaactt ggctctttgc accgggacgt tgacagaagg
    27301 atcgccgtaa atgagctgtc cgacggcgtc atccagtccg gggcacgctt gattgagctt
    27361 gtttagcacc atcttgccgc gctttccctc aaactgagcg tagtttggga agtcgacttt
    27421 ccagctcaag atggtgtttg cgggacgatc ggcgcgcgga tttatcgtcg tcttggtgat
    27481 gtcaaaaccg attccgtttg tgcggttcca gcggctgcct tcgtagcggg tcatcacgtc
    27541 gtagaccacg ccatcgctga caaacaccgc gggcaccatc gcatcccacg acgcacggag
    27601 catgcagcgg tttgcgctgc ttcccccagg cgttacgcgc ttgtcatctg gcgtcggcca
    27661 gttgacgttg tcgtacatct ggttccagtt ttcttttttg ataaagacgt tgtacgcggg
    27721 ggccgtcgat gctgccggcg gcgacacaaa gtacgcccag tgcgtgagcg gatcggacgg
    27781 acgcggcgaa atcacttccg agccgctgcc tttgtcgacc aagattcgat agcgaacgat
    27841 cgagttgttc ggctgtttcg gcaccgtcgc ttcatagaag ccgttgttgt tttgcagatc
    27901 gacggtggtg actggctctc cggtggcttg caggtcatcg acaaaccact gaagctgcgg
    27961 ctttgtaaac ggagccagcg ttgaaaacac cacgctgact ttcactgtgt cggctgatcc
    28021 aatgaccagg tttccacttg tcggcgtcac ggtcttgcgg gtgacaatcg tgggcggtgg
    28081 gccgctctgg ccgttcgctt gccccggatt tgcgccatca agcggagacg gcacccagtt
    28141 cgacagctcg cttgtcggca cgtcgtagct tacgcgctgg agcgaaattc ctttgtactg
    28201 gtgcggcgcg ctcgtgatgg gggtcggcag gagcgccagc cattcatcgt cggcaccaag
    28261 tgcatcggca ccaatcgacc acggaaactg atcggtgtac tgaaacgaat cctgcgtgac
    28321 gctgctgggg tcgaccaggt ttacgaccgc gccgccattg tccagttccc cggcgtagtc
    28381 tcccagcacc cgatcggtgg acaggttgta actgctgaca gagacgagag ccgccttgtt
    28441 tttggcgatc accacgtagc ccttggccgc gatctgggtt ccggctggga aggcaaactg
    28501 caccgccccc gtgagctgcc aacccgacag atccaccgcc gcatcggatc ggttgtaaag
    28561 ctcgataaac tcgtggtttt cgacgtaggc gttttccatc accgggtggt acatcacttc
    28621 ggaaaacagc acgctgggct tcgccatcgt cggaggtggc gtcggtggga catcggtccc
    28681 gttcatgctt gtcccgtcag aactacaggc aaaagacaga gttgcgatca cagaaagcaa
    28741 ccaaggctgc cacctcttcg atgtccgctt gttcatggat aagcctcgac aaattggtta
    28801 ataagaaggc gcgattcatc atatcacggt ggggctgtct gtttggtcaa gactcgacgg
    28861 aaagctccga caactgtgta ccgatcggaa catctgccaa gacaagctgc gatgggcgca
    28921 cctgccggcc aatttgctcg caaagaccac aggctaccga tgcgtctaga ccgcttgtgt
    28981 tccctttcgc aataattccc taccgtggca aagtttcgtc tgatcgatcg cctccgttac
    29041 agcttggacg cgaccctttc acgcggtcca ggtggtctga ttggctggct gtttgtgctg
    29101 ggtgggctgc tcgcgctgct gagcgccgcg ttcatcaccg ctaccgccag cgctcctgcc
    29161 ggaccttctg gcgtgcggcc cggcttcttt ctcttgtttt ggcagggctt tcaaaaaacg
    29221 ctcaacctaa acgtcggcat cggatcgctt ctgtatgtga tcggtctgct tctgccgacg
    29281 ctcggaagcc tgttcatcgg cggtattttc atcggtcttt tgaccggcgg aatccagaac
    29341 cgaatccgca atctgcgcaa aggccgatcg atggtgatcg aaaaaggcca catcgtgatc
    29401 ttgggctggt caccgcagat atttcagctc atcggcgagc tggtcttggc aaacgccaac
    29461 aagcgtgatg cctgcatcgt ggtgatgggt gaaaaagaca aagtcgagat ggaggacatc
    29521 attttggaaa aagtgccctc gctcggcagc acgcggattg tctgtcgcac cggcagtccg
    29581 atagacctta tcgatctagc gcttgtgaat cctgatgatg cccgcgcgat catcattttg
    29641 gcgcccgatg tcaaagatcc cgacgcgcaa gtcatcaaga ccatgctcgc actttctcgt
    29701 cggcagggca gtgcggaaag cggctgtccg atcgtggcgg aaatccgcaa cccgcgcaat
    29761 cgtccggtgg ccgagctggt tggcaaagcg cgcgtcaaag tcttgctggt tgatgaaatc
    29821 attgctcgca tcaccgtgca gacctgtcgg caggttggct tgtctgctgt ctatgcagag
    29881 ctgctgggtt tcgatggtga cgagctgtac atcgcgcaag aaccacagct tgtcggcaag
    29941 cgcttcgatg aagcgatgcg gctttacgaa gactccacac tggtcggcat tcagcgcgaa
    30001 ggaaagagtc tgtttaatcc gcccatggac acccagcttc gcaaagacga tgcggtcatc
    30061 gcgatttcgc gtgacgacga caccgtgctg cttgcgaagc aaaagccgcc aagtccaaac
    30121 caggccgcca tctgtgcgcc agtcagccat ggtgaatcgc ccgagcgcac cctgatcatt
    30181 ggctggaatc gtcgggctcc tttgatcgtg aaggaactcg atcagtacgt cgcccccgga
    30241 tcatttttgc tgatcgcagc cgagcttgcc gatggggacg ttgtgatgga aaagcagcgg
    30301 caactgctca aaaatcagac ccttcagttt tgcagcggcg atacaagtga tcgcgcattc
    30361 ctcgacggac tggatgcgca cacgtttcat catgtgatca cgctcggcta ttccgacagg
    30421 ctcggtccgc aggaagccga tgcgattacg ctggtgacgc tcctgcatct gcgtgatctt
    30481 gcggaacggc acggcaaccc ctattcgatc gtcagcgaaa tgtacgatcc ccgcaaccgc
    30541 gagctggccg aggtcgcgcg tgccgatgac tttatcgtgg gcgacaagct ggtgagccaa
    30601 ctgctcgcgc agcttgccga aagtccacag cttcagctgg aggaactgtt tgatgccgat
    30661 ggttccgaag tctatgtgaa gccggcgggg gactatgttc ggcttggcga agacatcgac
    30721 ttttataccg tcatggaatc cgccagtcgt cgcggggaag tggcgatagg ttatcgaatc
    30781 ctctccgagc agcagagtcg ccactacgga atgcacatca atcccaaaaa gtccgatcgc
    30841 atcacctttt ccgcccaaga caaaatcgtc gtgtttgcgc actcctagca cgttgtggca
    30901 aatgtcctgc tcaggttgac gcgctgcggt tcagaacagg gcatgaagac cccttttctg
    30961 gatcgcacaa gcgcgcgaaa cccaagtccg tccacaagtc caagtccatc gaaaagcgaa
    31021 aacccttcgc gcagatgttc cgcttcggtg tcgtgtacag aaggtggcag cgagcttccg
    31081 ctcgacgcga ttccgattct gccgggggga acttcatcgg tcatccagac caagtcgatc
    31141 caggttggcc cagccggcga tgcctatgaa caggaagcgg atcgcatggc cgaaacgctg
    31201 actcgttcgg ggtcttcatc gggttctctt tcacctgcac agtcgtcacc acttttgaca
    31261 agtccccaag ggatcttgca tcgcaagagc gaagacactg cgtccgcatc ggggcagacc
    31321 acccagcttg ttggcgcgtc gaagggacag cctctgcccc aggatctaaa acaggagttt
    31381 ggcagcaagc tctctgcgga tctaagcggc gtccggattc acaccgatgc gcagtcccac
    31441 gcctggaatc gctcgctgtt tgcgcgggcc tttacgtccg gtcaggacat ctactttggc
    31501 aaaggggaat accagccaaa cacgcaggaa ggacgcaaag tcctggcgca cgagcttacg
    31561 cacgtcgggc agcaaaatcc tggtcttttg ggggctcccg ttttgtcgga cccaagccgg
    31621 ctcgtccgtc cgctgcgcat caatcccaag gacaaaaaat actacagcga tgtcgcgccc
    31681 ggactgcaca gcgaatactg cgccgacacc gcagaggatt cctttctgac agcgacatcg
    31741 aatgccggac ttggcggcgg tcacacggcc atctacatcg agtatttcaa aaaggaaaat
    31801 ggcaccgatg tgccgcgcaa tcttcgtgtc gagctgtttc ttggaccgcg cggaagtggc
    31861 atcgacatca gcattcgtga ttacagctcg caggacatgc aggcaaagct gtttgaactg
    31921 ggcagtccgc acaaaaagac ctggaaaaag aagcgatccg atgccgagtc cgcgctcaaa
    31981 aaagcggaag acattcaaaa gggccaagcc gactatacgt actaccggct cgggcgtggt
    32041 ccgacgctga aaaacccggt cctcaactgt gcgcgcttcg gtcagcgggt tctgcaagca
    32101 gcaggaatcg atctccacat tggatctgcc aaactgccca gccggatcac cgatccgacc
    32161 cgtcaaaacg gcgcgatcag caacgtgtac acaagtgcag acctggctgc tgatgcgcaa
    32221 agctcgctgc cggattgctg tcgaaagcgc tccgagtttc tcaccgcggc aaagcgcgac
    32281 caaaacgatc ggtttggctc tgcgctggct gctgcgggcg gctttctgta ccagtaccac
    32341 caacatcccg aatcgctgct gcgctatccg gcgctggttg gtgccatcga ccagatccac
    32401 gactacgtaa agtccacgtc catcgaagag tttgtcgagc agcgccgcga ttccacagcg
    32461 accgtccctg ggagtgcgag tctggacttc attcgcaaag agctgcatcc ggcgctgcag
    32521 agcgaggtcg aacgtctacg caaggaagtc tctgccgagc taaaggatct cgatgatgcg
    32581 ggggcgccac gcggtgcgca tgccaagatt gattccgcgc tcaagaccgc gatgtctcga
    32641 ctcgatgaac tgatggcgtt ttccacatcg aaagagctgg tcgcggcgcg ggtcaaaaac
    32701 acgctcagca aaggcggaca gcttcaggat cgcaagtaca accagatcat gacgcaaggt
    32761 gaatatcccg ccctcattgc caactgggga tagcccagct gatctgtgtc gatggtcttt
    32821 tgggggcggg cgtgtgggga tgatcggttt ggttacggat cggtcttttc ccacgctttg
    32881 ccgcgcagct tgaacttgtc cggatagttt ttgtcatcgt cgctgggcga gacctggcgc
    32941 ttgacgaccg gctcgccatt tttttcttcc accgcccact tttcgtccca cttgccgttg
    33001 cgattcagat cgactttcgc ccgcacatac tttccacctt cggcatacag gttgactttg
    33061 tagggacgat ggggcgttgc gtcttttgct ttgtccgctg acggaggctg cgaaagcagg
    33121 tggatgattt cgtcatcaaa aggacgcagc gggacgatcg catgaccgct ggccgtggtt
    33181 ttttccgctt cggctttcgg cgccggtttg tcttcttcct tggccggtgc gggcggaggg
    33241 gttggtgggg caggggccac gggcgccgcg acagcaaccg gggctgcggg ctttggcggc
    33301 ggcaccgttt ccgacttggc aaagtagtac atcgagccaa acagacccga cacagcgccg
    33361 cccacgatca agttgcgaaa ggttgtaaat ttcatgtctg cccttgtgcg tgattcccag
    33421 caggggatgg ctttcgcatc aggccgcgct cgatgggcac cgtgcagtaa cacagcggat
    33481 acggacaggg ccgcggcgcc agaaacagtg cgaaagagtc cgacagaaaa tcccccagcc
    33541 gttccatttt atgccgacgg gccggataac agcgataggc cacaccgcgg tcgtcgagaa
    33601 tgaagtagcg cgcgcccgac cagcagggtt cgcctgtgta cacatgggcg agctggccgg
    33661 tgcgattgtg cccgccgagc gaaaccagct gctcgcgttc ctgctcgctg tattcgatga
    33721 ttccaccgtc gatcttttcg ggctggatct tgaacgtgac cccggcttgc gcaaactggc
    33781 tggccaagtg gaccagatca ggaatcgctt gccgggttgc gaccgtggtg acgttcaggc
    33841 tcggtggtgg caggctttca tcgcgggccg caagcagcgt gtcgcgcatc cactgcgcct
    33901 tctgaataaa cgccgcgaca tcggtcacat actcgcggtg caggctgcat gaaaacacgc
    33961 ccacccgtcc tcgcgccgcg tcgacaaact cgcgcagctt ggcaacactg gccgaaaagt
    34021 tggtgacaat cgaaatccgg tgtccgatct ctgcaagccc tgctgtgatc tcgtgaatcg
    34081 ttggatgcac aaacggttca ccaccggaca ttttgatttc ccaacgtccc ggcagcttgg
    34141 caaagccggc cagaaagcgc ggcgtgtccc ggctccagcg tccgcgatct tccatgaatc
    34201 gctgcgtgca gtagctgcat ctgtaattgc acgcggtgtt gatgttccaa gacaccacac
    34261 cttctgcggg aatcggcggc gccaacgtca tggtttcctc tgttaccgag actgcatcgc
    34321 tcgttgcgac atccgcagcg actgcgccgg aatcaggtct tcggcgtggg acttcggatc
    34381 ttcgttgtac ttgcgctcgg ctttgcggat tccttcgatc atgccgcggt tggcgggaac
    34441 agtgcaggga cagatgccat acggacacgg tgatgcggtt tcccacagcg tgagcgttcc
    34501 ggaaaacacg ttgcccaaaa aaccctgctt gtgccgtcgc gctgtgcggc agcagtagcc
    34561 gtcgccgttt tgcaaaagga caaagtattc gaccccggcg taacagagtc ggcctttgta
    34621 gcttggggcc atgttggcgc gatccggtgt tggatcttcc cccaccaagt cccgaatcag
    34681 cgcctgttct tccgctgaat acgttgcgat tccgccatcg actttcatga tctgcggaaa
    34741 cagcgtaagc cccgcgcgtt ccagaacatc acgcgccgca atcacctggg caatccgtcc
    34801 cggcaccagc acgctgttta tcgaaagatg ggcccgcggg tccatggtct gtttcagaat
    34861 cagcgccttt tcgacaaagc tctcgacggt cgtaaactcc aggtgcagac tcgccgacac
    34921 caccccgagc cgtccttggg tcagctcggc cagccgcgtg atgtatccaa tcggcgccga
    34981 caagttggtc agcaagctga tcgtgtgcgg tgtcttttcg ataagacccg gaatgatccg
    35041 atccataaaa ccgcggaagg caaacggctc tccgccggtc attttgatct cgaagcgcgt
    35101 ctttagctgc gcaaaaaaat cgagaaactg ctcgacctct gcaggacttg gatgtccttg
    35161 ccgatacacc ttgctctgga tgcagtagga acagtcgtag ttgcagatgc cgcagacctg
    35221 ccactcgatc gtcggactcc gcgtcatggc ttgcggcctg tgacttggta caggcgctcc
    35281 tgtgaaaacg cctgggcaaa gcgctgcgac agcgtgtgat tcatgttgaa cttgccgcac
    35341 tggtgacagg ccggaaaata ctctccgcga cggagctgcg cgcgaatctg gttccagcgc
    35401 gggccgtccc acagatcggg aaacgaacca tcagacagat tcccaatcac cacttccgga
    35461 ctacagcagt acagcacggt gccatcgacc aaaatccgcg cgtaggaata gcccatgaag
    35521 cagccgatgt cttcgatcgg ggccgttgct tcttcccccg cgcggagctg atccgcaaac
    35581 acagcaaagt tggcggtcac gttcagccgt tcggcttcct gtctggccgc aggcagccac
    35641 tctgccatca acttctgccg ctgttcatcg cttatccgga ctgcttcggt tccctcgcgc
    35701 agagacgcca gcttgaagtt cacttgctct gctttgtatt ccgcagcttg gcgaatcatc
    35761 gccaccagct cgtgcgcatt ttcgcggcag atcacatgga tgtgcttaaa ccgccgtccg
    35821 gcttcgcgaa actgcgccag catggcaagc acccgctgcc actcatcgtt tacaaagcta
    35881 ggatggaatg cgcgatacgt ctctgcgctt gccgcatgca gaccaatcag gagctgatcg
    35941 acccccagat cgacaatccg ctgcggctgg gcggccacca agttggtgat gatcgtcagg
    36001 tgcagaccgc gccgcttgac ctcggcaatc atgtcataca cctcgggatg ggtgaacggt
    36061 tcgcccattc ctgacaagat caccgcccgc agtccgccga gctcactcac ttcgtttagg
    36121 atgtctgtta ccgtcgctgc gtcgatccgc tgccgtttcc agcgctgcgg tcttggtaca
    36181 gtcaggtgcg gcgagtgatc ccagcacgtc acgcagtttg tattgcagcc attggtgatg
    36241 tcgatgtgca gcgtatccgg tcccgtcacc ggcctgccca agtgcattcc cagaagccgt
    36301 ggatcgacac gcttcatgac agcttcctga tggcagacgt gaccagatcg gaaagtgatc
    36361 cgccgctctg atcatcggac tgggcaaccg acagcgcttt gactccttcg tacaagtcaa
    36421 gctgacggct gcgcagggtg ccaagcagtc gtcgcagctc ggccaccgca tcggcagttg
    36481 gcagactccg ttcctgcgga agctgcttga gcagagactg tgcgcccggc cagtccgaaa
    36541 aacccgccag ggactgtaga agttccccca tggccacact tttttcgtcg agcccgccgg
    36601 ttccgatccg tcccgcgagc tgccgaacca ttcgcaccag cgccggctgg ggcagcgcgt
    36661 actgatggga cagtcgcaaa aggctcagcg cttcttcgca aggaatcagc tcgctggttt
    36721 cgccgtgcgt gctgcgcagc acatgcgcga tttcacagac ctgctctatc gacattcctg
    36781 attctgcaag ctgttcgatc agcagatcat ccaccgcttc cgctgccgcc atcactttgg
    36841 ggccgggcac aagcagagcc agcagtttct gcgccgcatc acgcgcgtgg ttgtgcggta
    36901 caagcgactg tgccgccaga ctcgcctgcc tgcggacgct ggggtccaga tacgaatcca
    36961 ctgcggcgct cagcttgtac agaaaaccgg ggctgtcgat cggaatccgc aggtgttgtg
    37021 cggcaccgcg ctcctctgcc agatcggcgc gcttgtcctg ttcatcggcg attttgtcct
    37081 gtggcaaaaa caccgtggga atcccggcat acaaaagctc gtgaaatgag ttatagcccg
    37141 ccgagcagac cgccaagtcc attcccgaca gcagctctgc cacgccgctt tgggtcagaa
    37201 acgtcatccg cgggccatgc agggaaggtc cgcggtacag ggggcctgcg ccaatcacca
    37261 gatgcaagtt gggtgtaagc cgcagcgctt cgcagacttt ttgcaggtgc agactcgcgg
    37321 tccgatcccc accaccgccc gcagagacat agaccaaaag atcatgttca gcgacgccga
    37381 agtgggccct caccttgtcg cgcggccaac actccacttt ttcacgaatc atcaccgggc
    37441 cggtgtactg aagctgtccg cgccggattc ctttgagcgt tccttgcgat tcctcttcat
    37501 gttccggaac caggatcaga tcgtacaagg gaagcatcgc ggcgaagtct ggacgcgata
    37561 ggaattcttc cttcaccgac cgatgaataa aggctttgtg ctgacacaga tccagcgccg
    37621 acaaaagctc accaaacgat ccgcgcggaa acgtatcgac cagaaacaga tccggacgca
    37681 gcaagcccaa gctatgccag acccactgct tggccaatgc caggtacgtc gtcttgtcga
    37741 tgccggtatc accgatgatc gttttcgacg gcagcttgaa gctcgcaaag cgttcggaaa
    37801 acagcagaga gtccgcttcg ctggtggtaa gaaaataaat ttcggcgcgc agatccagca
    37861 cttgggtgta gcgacgcagc cagcgattga tggcacacag ccgcgtcagg tgtccggcac
    37921 cggaaccatt gaccgcgtaa tttacgatcc gcagtggcga aggcatggaa taggaagctt
    37981 aggaatagtc gctgctgcgt tcgtggtggt tgccgctgcc aatcacagcg atgacggcat
    38041 ccccgatgac gccatcatcg tccacgatca gatcatcatc atccacgtct tcgacgatgc
    38101 ccgacttgtt ctgtttgggc cgacggtatc tgtacgtggg atgcagcgct ttgaagcgcg
    38161 ccacttcatc gatgtagctc gctttgactt ttccgtccgt gatgagatcc caccacagaa
    38221 agtcttcatc gagccgcccc atttcgtagg caccaaaggc caggatggct gtctgtgcgt
    38281 cgcgctgccg ggccaaaact tcctggctgc gtcgattgcg ttcggcaatt ttggtcagct
    38341 ggtcggcgga gatccgctgc gacgcatact tgggccgctg gtacttggtg atttcgcgat
    38401 ccagcttctg aatcgtctgt tccagcgccg gtcggctgtt gtccagaagc tgcgtcgatg
    38461 tctggcgcag atactccgcc tgtttgcgca gaccgaaata gcgcttcgcc atcgtttcga
    38521 catcgtggtg ctgggccaag cggtcgccaa tcgtcacaaa gtcgagatcc gccaggtagc
    38581 gctccagctg gatgcgcgct tctttgagaa actgctctgg catcttcgcc agctgttctt
    38641 gcagcccgtg ccgaccttcg gagagcgcct tttgttccgc cagcaagctg tcatgttcgc
    38701 gctcgatggc ggcgccacgt tccacttccg cgcgcagctt ggagagcttt tggtcataca
    38761 ccgcgacgcc atctctggcc gccaggtact cgctgcgcac gtccacaaat gttttttcac
    38821 cgccaaattt ggcgacgatt tcgtccccgg ctttccagtc cgaaaagtag ctcaggcgcc
    38881 agaacggaac cgaatactcc ggcagtccgt acttgctttc caagagccgc tccatccgtg
    38941 ggtgctcgca gcggcgcaag acctcgcaaa gcggctcgcg aaactcctcc agctcggcaa
    39001 tctcgcgggt cagcgtgccg gtccgtggtg cacgcagaag cagccgattt tcaaactcgg
    39061 gcagccgttc aatgtcatgt gcgcgccgct caatctgcac cgcgcgatcg cggatctgtt
    39121 ccccgagctt gtgctgcgct ttttgcagct cgatcgccgg attcacaatc gcaaacgtcc
    39181 ggtatcccga aagaccgatg gccgcttcga tgctttcggg cgtaagcgac ggcagcatcg
    39241 cggcagccag ctccgaaagt gcggtttctt gtcggttgac cagggacgac acctcgcgcc
    39301 gcgccgcttg ttgctgcgtg ttgagctgcg cgagcgtctc tttcatttga tcacgcgcct
    39361 tttgggcatc gtgaacatac gtgacactgc tgaagtaggc ggccatggtg gcttactccc
    39421 tgttgccgcg acgtcgtgtc gccaccatgt ccaggaagcg ctcagcgtac tgtcgcagct
    39481 cgtcttcggt tgcgacgtcg tactcatcga ccaaaagaat gaacagctcg cctcgggcga
    39541 tgcgctgctc aatcttcgcg atggtttctt ggcttagctc ggtgcggtcg ccggcttcgg
    39601 tggtgatgaa ctcaaagccg acgcgggcca cagggtcaaa accatcgacg tgaaatgcaa
    39661 cgccgtcttc cgaaaaaggc acgttgtcgt ggatgttgta accagcggcg aggaaggatt
    39721 cccgaaggac ggcagagccc tgcgattcgg tgagcatgtt cgcgtatgtt atcgcgaatg
    39781 cccaccgaga ggaagccggc gaaggttggg ggatgtttag ttgtcgggct gaatcaccca
    39841 ggtctcgcct gagcggagca gcttggcgac gtcgcctttg ccgcggctgg cctgcatctg
    39901 ttcgcgctgc gcggtcacca ggtcttcata cggcgcacgg cttacgttgc gcagcacgcc
    39961 gacgggcact ggatagtttg gccactccat ccgcgacagc atgaacgcat acgacggatt
    40021 ttcaatcgtc tcgtcgtgga cagcaatttc ggacagcggc acttggccgg ctccgtcacc
    40081 gacatcgacg acttcgggaa cgaagttttt gccgatgcgg atgcccttgt ccttgttctt
    40141 gccaaacagg agcggcttgc cgtgctctag gtacaaaacc cggtcggcct tgtgggtctt
    40201 gtcggtgatg tggacgaacg catcgtcgtt gaacaccggg cagttttggt agatttccaa
    40261 aagcgtcgtg cctttgtgtt cgcccacgcg cttgagcatc gcctgaagat gctgaatttc
    40321 ggtgtcgaac gagcgcgcca caaacgaacc ttcggcacca atcgccaaag acagcgtgct
    40381 aaacgggtgg tcgagcgagc ccgccggtgt cgacttggtc tttttgccca tttccgacgt
    40441 cggcgagtac tgacctttgg tcaggccgta gatgcggttg ttaaacagca cgatcttgac
    40501 gccgatgttg cggcgcagtg cgtgaatcag gtggttgcca ccgatcgcaa gcgcatcacc
    40561 atcgccagtt gccagccaca ccgtcagatc gggccgcgcc agccgaatgc ccgtggcaat
    40621 tgtcggcgcg cgtccgtgga tggtgtgaaa accgtaggtg ttcatgtagt aggggaagcg
    40681 gctcgaacaa ccgattcccg atacaaacac gatcttttcc ttcgggatgc caaactccgg
    40741 catcactttc tggacttgcg ccaggatcga gtagtcaccg cagccggggc accagcgcgg
    40801 ctcttgattg gatacaaagt ccttccggct gagagccggc ttgacttggg ggttttctgt
    40861 ggtggacatc gacgatacct cttccactac ttttgaccgc cggggcagcg ctcggcaatc
    40921 gcttggcaca gctccgcgac tttgaacggc ttgccctgga tcttgttaag accttcggca
    40981 tcaaccaggt actccgcgcg gagaatgcgg accaactggc cattgttcat ctcgggcacc
    41041 agcacgcgct taaagccctt cagatacgcc gcgaggcgcg ggttgagcgg attgagccag
    41101 cgcagatgga cactcgtgac cttgtgcccc ttggtctgca tcgtctgaac cgcttgcgtg
    41161 atcgcgccat acgtcgaacc ccagcccacg accaacagat cgccttcggg cggtccgcag
    41221 gtgataagct cgccggcttc cactgccacc cccgcgacct tggccgcgcg ggtcttcacc
    41281 atctggtggt gattttccgg gtcatacgag atgttgccgg tcaggttgtg cttttcgatg
    41341 ccgccgatcc ggtgctcaag gcctggcgta cccggacgga cccacgcgcg cgccagcgtt
    41401 tctggatcgc gaccgtagac ttggtagccg ttcggatcgg tgcggaactg gaccgggaac
    41461 ttcggcaggc tcgacagctc cggcactttc cagggctccg atccatttgc caggtagccg
    41521 tccgtgagca caaacaccgg cgtcatgtac ttgatggcga tgcgggccgc ttcaatcacc
    41581 gcgaaaaagc agtcgctggg cgtcatcgct gccagcaccg gcaccggaca ttctccgtga
    41641 cggccataga tcgcttgcag cagatcggcc tgctccgtct tcgtcggcag acccgtcgaa
    41701 ggtccaccgc gctggacgtt cactatcacc atcggcagct ctagcatcac cgctaggccg
    41761 atcgcttctt gcttgagcga aacgccggga ccgctcgtcg ccgtgacacc cagcgagcct
    41821 gcgtaggccg cgccgatcgt cgatccgaca ccagcgatct cgtcttcggc ttggaacgtg
    41881 gtcacgccgt aattgaggaa gctggacagc tcatgcagga tgtccgacgc gggcgtgatc
    41941 gggtagccgc cgaaaaagac cggcagctcc gcgagctgac cggcgcagac aatccccagc
    42001 gccgtggccg agtttcccgt cacgttgcgg tacagacccg gctcgatttt cgccggacgg
    42061 acgtggtagg tctgcgcaaa cagctcggta ttttcgccgt agctgtagcc gcctttgaag
    42121 gcgcggatgt tcgcttcggc cagctcgggc tttttggcaa acttctcttg gatcgagcga
    42181 agctcgcgct cggtttcacg ctggtacatc cagaacatca ggcccagcgc ccagaagttc
    42241 ttgcagcgac cggcgtcctt gttgccaagc ccgctgcctt cgacggccag ccccgtaagc
    42301 ttcgagatgt cgatttggat cagccggtac ttcttatcca ggccgccatc ttcgagcggg
    42361 ttgctgctgt agccagccag ctcaaggttc ttttggccaa acgcgcccgt gttgacgatc
    42421 aggatgccgc cagggacaag gtcaggcagg ttggtcttga gcgccgccgg gttcatcgcc
    42481 accagcacat cgggctggtc accgggggtg tagacctctt gcgatccaaa gtggatctgg
    42541 tagcccgaca caccgaacag cgttcccgcc ggagcgcgaa tttccgccgg atagtctggc
    42601 agcgtcgcca ggtcgttcag tgcaagtgcg ctcgcgcgcg taaactcggt ccccgtaagc
    42661 tgcatgccat cgcccgagtc accgcagaag cggataacga cggactcgac gacctgcatg
    42721 gccggtgggt tgggcggttt cggagcggtt tccccgctct gggtttggtt ttccatggtc
    42781 taaggactcc tcagggtcgc ttgctcgtca cagcctggag cagacagcgt tgcgcagaaa
    42841 ataggtatga cgagagcgaa taattcacaa gggacttggt atccctgttt gcaattaacg
    42901 aggtagcacc cgtgctagca gaaccgaaag aacgatggca agtgtcagca gcgcccacca
    42961 gatccattct ggctgcggcg cggccacggg ggcagggggc ggtggcgtcg gagccggcgt
    43021 cttggctggc tcggcaggct tcaggcttcg caagggcaca ccgggcgaag actgatagtc
    43081 cgtcttgacg atctccatgg gttcccagat cggattgctc tgggctgggt ttttcaccgt
    43141 tccacctggc gtgcgaatca tcgtcgcagc ttcaaccagc cgcaggacag atgcttcgct
    43201 ctgctcgggc tgggtgcttt gtggcacgga ctccggcggc ggcgtcgcaa tcgcatcggc
    43261 caccagcgca tgcagccgac ccggtttcgc cgcaagatcg ggcgccagcg ctggcacggt
    43321 cggcccactt ggggcattga cggtcggcgc gtgcgggttt ggctccgccg gtttgcgggg
    43381 cggctccact gccatgcgcg ccgactcgac cggaacgcgg gccgattccg caaacacccg
    43441 gcctgactcg acaaacaccc ggcctgactc gccgtagtat cccgcctctt gcactttgcc
    43501 atcgacggtg acttgttcac cgctcaccca cagaagccgc cgtaacatgt cggccacctg
    43561 cgccatcgtc gggcgttcgc tcggctcttt ggccagcatc ttccacgcca tctcgacaag
    43621 tggcggcggc aggttgggca caaaatcgct cagccgcggc ggtggctgca gcatgtgcat
    43681 cgccagatat tcgcctggct cggcggccac aaacggggcg cgcccggcca ggatctcgaa
    43741 caagatgacg ccgagcgagt acacatcgac cggcgctccg atgcctttgc tgccgcggca
    43801 ctgctccggc gacatataag ccggcgtccc catgatgtaa tttagctggg tccgcaccgc
    43861 gccgcggtat tcgtcggcca tcttggcaat cccgaagtcg agcagcttga tccgctcgcc
    43921 accgggggcc tcgccatcgg gcacgatgaa gatgttgtcg ggctttaggt cgcggtggac
    43981 tcactttccg tcgagacaag gccgtccaat tttccccgcc gtcggggctg gtgaaaaagg
    44041 agaaaaatca gagaggatgg agtttcgtga acgctcgtcg gacggagttt gctaacagga
    44101 aacacaagaa acgtatcaca gacatcataa atgaatgcga aaataaaatc gtgtacatct
    44161 atggtccata ccccccagcg gaggggagaa cgaagtggcg tgtggtgatt tacgacccga
    44221 gcacgggcaa gcgcaagagc gtctgcgccg agacgcgggg cgcggcggat ttgctagcgg
    44281 tgcagcttcg gcgtgagacg gagacgaagc cggttctgac ggtgtatgag gcgctcgccc
    44341 agtggatcga cgagaagcgg gctgcctggc gacaccctga atcggtcgct cgcaacgtcg
    44401 aaaatcgagt caaagcgtgg attcccgatg tgcctgtcga tgagattgac gcggcgcagg
    44461 cgtcggcact gtacctggcg gccacgaagt ccgagggcag gttcgggccg atcaaggctg
    44521 caacgcatca cggctacctg aaggtggtca aggaactttg gcgctggctg ctcgaccggg
    44581 agttcatcga gaaagatgcg ttcgcgaagg tgaagccgat cggcaaggcg aacgcgggca
    44641 agcagcagc
//
LOCUS       DAORGN010000014        43997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_288_length_43997_cov_6.151632, whole genome shotgun sequence.
ACCESSION   DAORGN010000014 DAORGN010000000
VERSION     DAORGN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 43997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43997
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_43997_cov_6.151632"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..739)
                     /locus_tag="PKL17_03040"
     CDS             complement(2..739)
                     /locus_tag="PKL17_03040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNK43729.1"
                     /translation="MMRLEQAGRTHVGLVREKNEDAMLLAPDIGLYVVCDGMGGHVGG
                     QVASNLAVKTIDQVIRGGQRPPPEDREILVTAIKSANRAIFTQARQDPTLHNMGTTVV
                     GIRQNGDTLQVCHVGDSRIYRLVYSSGELIQVTRDHSLVNLYEDNPELARRYGPPNDN
                     VIVRAVGLHEAVEVEHQTVNMQLGDVYLLCCDGLTDMVDDWILREVMSEAATAPTLDE
                     SCDALIRAALSFGGVDNTTAILLRVAQ"
     gene            883..1716
                     /locus_tag="PKL17_03045"
     CDS             883..1716
                     /locus_tag="PKL17_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43730.1"
                     /translation="MSVHFDGGRCHVRCPFCYLGEREGTAQSGLPVELLCTSLQTLAY
                     DELAIALSEPVTPVLGPLRRMIDVAVEKKRRVTLTTTIAAAIGLPPDAWRGVDRLNLS
                     IDPLKGQFRAPVGQVVPEDVGEALRLLPSSVEKVLIVTLVSPEFADTLVQGLLAHLLA
                     LPEVHRVALSGLKPPPPFCSQDFWLRLLARLGPLLRDHLDKRLFLDCYVAARILQVGG
                     CPARPDLSPTSKEDAARGALAFRSCVYQSQADFIASRAEDLDMYLQDFHVPARCPFPI
                     H"
     gene            1863..3131
                     /locus_tag="PKL17_03050"
     CDS             1863..3131
                     /locus_tag="PKL17_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNK43731.1"
                     /translation="MSSALAAKCPNVHFVLDRSGSMSTSVPGAGPGATRWTVAKDAVN
                     KVLDKWDGQFPIGMSIFPPTGGSGCGSDLVTAPAYSSKAKIKMAIDANGPNGGTPSGS
                     AMRDAQMIKELRDPERKQYIVLITDGGPGCGGEPDSCSGTVGMIRQAAMASPAIGTFV
                     VGFGGGLSSTEAQCMTQMADAGGKPAMTPEKYYKADNAADLNTALSNILEIITGGGDV
                     GMGGLCDDTCYSNGCPTPGDLCVKGVCKANPCAGVSCGAKNSYCSSDGSNSGICVSAC
                     TKACPKGTRCNMGTCAPDPCPYYCGAGLVCDTTQKRCVPDPLCQNLPPEKQCKGTSRC
                     RAGECVDDPCRWTRCPTGTRCVAWEGSCELIPTDNPPDDNPKMPGDVDVGSRVGGCST
                     VPGGANAVSMSAAALMLGAMVAMRRRRRAE"
     gene            3353..4825
                     /gene="zwf"
                     /locus_tag="PKL17_03055"
     CDS             3353..4825
                     /gene="zwf"
                     /locus_tag="PKL17_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNK43732.1"
                     /translation="MVIFGASGDLTRRKLVPALYSLARERLLPSGFAICGFARRPLPF
                     ADDMKTAVGQFARRRPVDESLWESFGSAISYVSGDFDDPAAYERLRSHLLDIDLARGT
                     RGNRIFYISTPPESFETILRNLGRAQLISPGERPFTRVIIEKPFGRDLASAEQLNRVC
                     LDVLREDQIYRIDHYLGKETVQNILLFRFANSIFEPLWNGKYVDHVQLTVAESIGIEG
                     RGNYYDQAGTLRDMVQNHIFQFMTLMAMEPPVAFESGAVHDEKVKVLRALREVPLGEV
                     DRFTVRGQYRDGFHQGQPVPGYLAEQGVSPTSHTETYVALKLFIDNFRWGGVPFYVRA
                     GKRMARRVSEIAITFKQLPHTLFAGPQGKLSHEPNVLSLRIQPDEGIVLKFLSKVPGP
                     TIEPRPVTMDFRYGTSFGTEPPEAYERLLLDCMLGDSTLFARGDEVMASWRYCDRILR
                     GWAEQDARNRAPLPSYEPGAWGPEPAATLLARDGRVWRRP"
     gene            4822..6066
                     /locus_tag="PKL17_03060"
     CDS             4822..6066
                     /locus_tag="PKL17_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HNK43733.1"
                     /translation="MSENGDYRQNERREGWAVPLDAVSAFTQGASVSVAPDRIEQELL
                     LLWRKASERAQAKGAAFAVTRACQWNLIVHTHGEEELLTMKQVLDTVSETVPCRALVL
                     HESDASTFSGVIGDDGAAMHASVEANFRDSPSGRREIVAEEITIETPQLHSRRLPSLV
                     RSLLLPDVPTTVFVHNPALDADWLRPLCADADRLIFDSGRLRSGGELGQLAQVLPELW
                     PGHEMQVELMDLGWLRLWPWRLLLSSLYDTPQSRAGLAQLREIEIGHTAGFMAQALLV
                     AGWLLGRLGLVQTGTLESDGSVGLLPGAGSSVQCPVRLRLRQCEVACRATGISHVTLR
                     QADYELSAQNDACGGGIELRSPFQPARTQPLRGRPDAELVIAALGVGGRDPLMYEALQ
                     LGASLLLGDAMLRAEKQLEVRW"
     gene            6063..6890
                     /gene="pgl"
                     /locus_tag="PKL17_03065"
     CDS             6063..6890
                     /gene="pgl"
                     /locus_tag="PKL17_03065"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNK43734.1"
                     /translation="MSGRAPEIWIGEDGEALAREGVRRFVDVARRSIAARGRFVVALA
                     GGNTPRRLYQLLSHVDSIDWGRVHVCFSDERMVAADSSDSNFKMAHDSLLGVVSIPPG
                     QIHAIPTAMSVDEAALRYDQTLREILPKNEPRLDLALLGMGADGHTASIFPGSRLLAG
                     VDDDFLLDDPETGPLRNDVPTPSLDSFSSERLCAPIYDAPKPPRERVTMTPYVLNLAR
                     QVIVLVAGDDKVAAVSAALESDARVAEIPIRAIAPATGELVWLLDRKAGRALGRPMR"
     gene            6927..7562
                     /locus_tag="PKL17_03070"
     CDS             6927..7562
                     /locus_tag="PKL17_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43735.1"
                     /translation="MSTARCLCLLGLILASSACTEKMPKLLPPVFSDNFDRTELGPSW
                     KSTAPPGTYRIENGELVVSGGKNHPLWLVPALPRDAVIELDAYSKSPDGDIKVEAFGD
                     GQSFAKTVSYTATSYVFIQGGWQNRLAALCRMDEHADDRKTRGDFKVVPGQRYHWLIA
                     RKDQLVRFYIDGKLALEMDDLVPLEGPGHSFFGFNNWEAEVHFDNLVVRPY"
     gene            complement(7614..8411)
                     /locus_tag="PKL17_03075"
     CDS             complement(7614..8411)
                     /locus_tag="PKL17_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43736.1"
                     /translation="MSRLLVLRSSEPVRARGLFQRVAGFVPQAALVDAVPAGDDAPAA
                     PGKTVSHDQGFYIDGEPLIQRGKSEELSAYRATANLRTDQLMSVIGEPPLQQGVFRYR
                     QFLCAVVGQLQPTAARAHHTADLPVSLRDNLRGRSEAELFFLGLLGNLHDADPAYLTT
                     AELSAEAAVAVLAKMLRQAVPDEAGTGVVAALSHGDMLVIARRGQSSLHYQVTSEGQT
                     PAQGQKVTLAVAEVGSTPVFGEPNAVPDGKALVLAARGSRPMLITLA"
     gene            8533..10536
                     /locus_tag="PKL17_03080"
     CDS             8533..10536
                     /locus_tag="PKL17_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNK43737.1"
                     /translation="MLKTIFNIRLLFLIGLLWPLAGHASDDERAMQTQQVAALVDYVA
                     ADYAGAVKDGQVTDESEYAEQRNLLEEASKLAQKLGGSDADQARLSAALAEVRSAVSA
                     KQPATLVGERCHGVRQILKDAYQLKMVPSSKVSSSQAAAQFQMLCASCHGVTGRADTE
                     AARKLTPPPVSFFDEEKMPGVAPQLAFHALTFGLNGTGMPSFGSLPAQQRWNLAFYVV
                     GLRHGTPGQDKPSALSSHQAVAALSLADLAEQSDKELCETLQKGGLDKSRCGEALSVL
                     RTQTPFVSIVEGPMHFEKARALLVDLRGAVERGDGALAQRLAISAYLDGVEPNEASLR
                     IEQPELLRKIESTYLSLRQSVDPKSGVSKEAVLAKIVEAQNLLAEAERGSGGTVAAFL
                     ASLTIALREGLEVALLIAALLAFLRKSGQGHLARLVHMGWMLAVPAGFLTFVAVGALV
                     SGAQRELAEGMMTLLAAAILITMTHWIIGAKEARHWLGFLRRRVEGAGEQAALSQKLA
                     LLGLSFFAVYREAVEVVLFYRSLLVSAGPQGRSDVIWGAVVGVVVMFAVVLIVGRIGR
                     KLNPRPVMLVSSALLALLALVMTGNGVHALQEGGYVGATLIHAFGREWGGVPVLGLHP
                     TWQVVLSQCVVALLLVVPSLIDRSRRRDGDAPLPPVGHLPSKA"
     gene            complement(10601..13150)
                     /locus_tag="PKL17_03085"
     CDS             complement(10601..13150)
                     /locus_tag="PKL17_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43738.1"
                     /translation="MSQLLSKPLAGRFELLSQVGEGGMGTVWKARDLSTKQLVAVKLL
                     NLDVSQPEEVARFEREVGLLAELRHPGIVRYVAHGQADTQAFLAMEWLEGQDLAGRLL
                     QKPLSTAEAGILLRRVAEVLSVAHQNGIIHRDVKPSNLFLCHGRPEETTVLDFGVARR
                     GIQLTRLTRTGVVVGTPEYMAPEQARGRRDITYSADLFALGCVAFELLTGQQPFAAQH
                     ITGLLTKILFEPAPRLHTFRPDLPQPLDELVAELLEKDPLHRPQSAADVYERLCQIEF
                     TAGDSQPRPPPVQIAAISGDELLLTTLVLATAPKSEQRRITTLDDSQAASLRMARHQV
                     LDHLAAIGVEAALLADGSLSATIVDAFAATDQVQLAARTASILRSELVHWPVVISTGM
                     CKRHGLFSTGPGFEQSQRILQAALDHDSHGAIWLDELSAELLHDRYLVSAAPGGYFHL
                     GARLSEAVLRTNRLPLIGLDRELSLLHSAIHGASEDEVSRAVLVLGAPGSGKTRLYEE
                     LIGRMRDAGEDFAHLSVRLAPQNRSAPFSALIGWLRSVDQKSLDDHSERPLWQKWLFD
                     RHPHDEAAQHQHVLSALLHHALPRPPSATPLTLPGADSVADALAALFVAAAVGRPLLL
                     VVDNVHWIDAASRSALSTVFLRLVDTPILLLGLGRPEIDELLPRFLEGQPIDRVRMGR
                     LPGKSADKLLCHLGIENSQRREELAHAGCGLPLYLLWLAKDETSGQTIGTRAVIAMMM
                     SRAHRLDPQAKQVLRAAALLPDRFTQGELLQLLPTDFSLSLLDRTLAMLIDHEWLEWI
                     RPTQVDDELTLCFRSPLLLRAMLEIVTDEDRLIGTSIVAKLRKRQSPPSQP"
     gene            13344..14603
                     /locus_tag="PKL17_03090"
     CDS             13344..14603
                     /locus_tag="PKL17_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNK43739.1"
                     /translation="MAEQNYLAPGIQQISTLAGIAVAGGEGALIEDVDGNRFLDWVAG
                     IGVASIGHGHKALAQALSQQAASIVATSFASPARLELLARIVEHAPHPSLNRVQLYSS
                     GAEAVESALRLARAATGHSEVLAFWGGFHGKTLGVLGLMGSEWKHGLLPVVPGQHLAP
                     YGDCRSCALRLSPDSCRMACIDFLRDQIRLSTANRLAAIVVEPIQGTAGNVVPHPSFL
                     PALAQVAHEHGALLVVDEMITGFGRTGDLWASTAAGVVPDIVTFGKGVAAGFPLSGLI
                     TRAELAASRPWADPSHSSSSFGGNPLACAAANATTRIIIDENLSENARRVGERLFAKL
                     TELHARFGCLSGLRGRGLMLGFDLVSDGKLWPKDRCLALFRACLSRGLLTMAYTPRVR
                     INPPLVLSLAQADESIHIFADALASVA"
     gene            complement(14604..16289)
                     /locus_tag="PKL17_03095"
     CDS             complement(14604..16289)
                     /locus_tag="PKL17_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNK43740.1"
                     /translation="MQQKLSELFAFLAKWLKPSQKLSPRLLLTVVISLLLALLLPLPR
                     LLLDGLLFTSWLSATLWLIAAVWAGEPLRLPQLPTLLLLTLLGRLCLTLALGRQAVAS
                     GQPGLLAALLQSTMGASEVSIGLLLLFALLVAQYVLLARGGERVAEVAARFVLDAMPG
                     RQAAIDADVRQGALGVVEAQAARAELTREAELYGSLDGVLRLLRGDVLLSVLLWLGIG
                     VAQMVSLSQTSELPTSEAAQQAARLVIGVGLATQLPVMLVTTAAVLLLLRSTSATAPS
                     DGATMGPTLRVIASAAIQHSEAQLVSVVERLLAHIGLGSVGFVIEFAPLTGTQFRVMI
                     HGALLAQRTVLSNERPDAVLQTMLYDLAAQLLSLDGLRKEIAKIGSERPALVSEVVPK
                     RLPLGRLLWILRRLLSERVWPLPIAAVLETISTLPELDADADGLIEQVRSGLGPYLLS
                     GFWRNERSDGENGLPVLVLSSDVEDVLRDARRSGGGTRLALEPDLRQEIIDAVLTAKT
                     RAPYAVLLCQKDLRRHVESLLAATPQALPVLAYSELPPQLPLLVISRVGPGTG"
     gene            16425..16889
                     /locus_tag="PKL17_03100"
     CDS             16425..16889
                     /locus_tag="PKL17_03100"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK43741.1"
                     /translation="MGSFSLSYSTVKSWAERLKLPFRASDEAGQIAVHCRLLDSDVPL
                     LFIPRPERDMLTVAVTLPFVVKTERFAAVTAALSTLNVRSYMGAWILNPERGEVYFRV
                     TLPTLDTAFSDDALRFVARVVLSSAEAMAQPLYSIAHEGASPEIVAKTRSAT"
     gene            complement(16908..17993)
                     /gene="amrS"
                     /locus_tag="PKL17_03105"
     CDS             complement(16908..17993)
                     /gene="amrS"
                     /locus_tag="PKL17_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNK43742.1"
                     /translation="MTITESSHPARYFHSLPDGRIQCDLCPRDCKLADGQRGLCFVRQ
                     NLGGHMILTTYGRSSGFCVDPIEKKPLAHFYPGSSVLSFGTAGCNLSCKFCQNWDISK
                     SKEMDRLSDQASPDQIARAAVAKGAMSVAFTYNDPVIFAEYAMDTADACKQAGIKTVA
                     VTAGYIHKEPRADFFAKMDAANVDLKGFSEQFYMRLTASHLQPVLETLEYLVQKTSVW
                     TEITTLLIPGFNDSDEEITRLSEWVATKLRPDVPLHFSAFHPDYKMMNVPPTRPETLQ
                     RARATARRVGLKHVYTGNVHDVEGDTTICAGCGESLIVRDYYQILSYRLDPSAGCPRC
                     GQKLAGHFAAKAGDFGRRRLPVRISAF"
     gene            complement(17990..18550)
                     /gene="amrA"
                     /locus_tag="PKL17_03110"
     CDS             complement(17990..18550)
                     /gene="amrA"
                     /locus_tag="PKL17_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNK43743.1"
                     /translation="MPSQAEGLALCQYARASIRAALGGPDAVPPQNPAFDKDGACFVT
                     LYRDGELHGCIGSLEAHQPLVADVAENAVAAALRDPRAMPIGLGDEQQFRVEVSLLSP
                     LVRLPVKSRAEAIAALRPHKDGVLLRYHGRRGTFLPQVWDELPEPREFWRHLLRKAGL
                     PTDFDSPELEVYRYTVDKWIEEPTTP"
     gene            complement(18534..19340)
                     /gene="amrB"
                     /locus_tag="PKL17_03115"
     CDS             complement(18534..19340)
                     /gene="amrB"
                     /locus_tag="PKL17_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNK43744.1"
                     /translation="MQTAIRPPAVAGLFYPREAHILADTVSHLLANATPRPPFPKALI
                     VPHAGYVYSGPIAASAYAQLQGAHHIERVVLIGPAHRVPVHGLTSPGASALRTPLGDV
                     AVDIETLRHLPSVLANPRAHAQEHSLEVQLPFLQKVVPHAKVVPLVAGWASAPDVGEV
                     LDSLWGGPETVIIVSSDLSHYLPYLEGQSEDRHTADLIVSPPAESDVLTPEQACGCVG
                     INGLLWVVRRRKLKVRLLDLRSSGDTAGDKAHVVGYGAFAVYEDSHAKSS"
     gene            19569..20090
                     /gene="nuoE"
                     /locus_tag="PKL17_03120"
     CDS             19569..20090
                     /gene="nuoE"
                     /locus_tag="PKL17_03120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNK43745.1"
                     /translation="MALQFSPQADAKISDITRRYPNTQAACLPVLHLAQDEFGYLSEE
                     VIELVAARLSIPSSHVFGVATFYTMFHREPVGKKVLMMCTNVSCMLTGAYETLRKLEQ
                     RLGIKAGQTSADGEFTLIEEECLAACADGPAMICGDRYFLRLTADKVDATLDECRKLP
                     AQHGHHPHSDKKH"
     gene            20094..21461
                     /locus_tag="PKL17_03125"
     CDS             20094..21461
                     /locus_tag="PKL17_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNK43746.1"
                     /translation="MPADRNTKVVTRLFGVQNSNRIDVAMANGAYKRLPPAFAQQPNA
                     ITEEVKKSNLRGRGGAGFPTGMKWGFIPKGADTVYLVCNADESEPGTCKDRELLFWDP
                     HLLIEGMIIASYALGCRHAYIYIRGEMIREAAVLQKAVDEAYAKGFLGRDQQTAIGPV
                     KFDLTVHRGAGAYICGEETALLNSLEGKRGWPRLKPPFPAVKGLFGKPTIVNNVETLM
                     NVPYIIEHGSQKFAELGVGKSGGTRMLCMSGHLKRPGVFELPIGITFRQVIDEVCGGT
                     HSGKRIKAVIPGGSSMPPLDASELDVPIEFDALMNDPRIKPVQYKPGHNFELAPGKPL
                     RAMAGSGGIVVMDEDTDIVSVCARIMRFYAHESCGQCTPCREGTGWMSKICTRLADGY
                     GQPGDIDLLSSIAYGIAGNTICPLGEAAAWPMMGFITKFRPDFEARLAQSRGLPPAGY
                     HGAHP"
     gene            21458..22162
                     /locus_tag="PKL17_03130"
     CDS             21458..22162
                     /locus_tag="PKL17_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNK43747.1"
                     /translation="MIALAQTDLASLLMQAAQVQAVPASSLSPTGWTPAWAFWILSAL
                     VVIGAISTIAQRNLISAVMSLVATFFGLAGLYATLSAHFLAAIQVLVYAGAIMVLFVF
                     VIMVLNRDEAERVSDRGLLDKAVGAGALVYLAVRLGEVIASPVGPVSRMSKPPADFGT
                     VAKIGEYFFTEFLFPFEAISILLIIAVIGAVVVARTVVSRPTTGYEVPTAAEHAGEGG
                     AEDAAGGDSHGGHGGH"
     gene            22167..22496
                     /gene="nuoK"
                     /locus_tag="PKL17_03135"
     CDS             22167..22496
                     /gene="nuoK"
                     /locus_tag="PKL17_03135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNK43748.1"
                     /translation="MQLILQNLLAVSLHHYLILAVVLFGIGTFGVFMRRNALTTLMSI
                     ELILNSANLTLLAFARSHQDMRGQALALLVIAVAAAEAAIGLAIVVGVFRNRHNTNID
                     ELNLLRY"
     gene            22515..24821
                     /gene="nuoL"
                     /locus_tag="PKL17_03140"
     CDS             22515..24821
                     /gene="nuoL"
                     /locus_tag="PKL17_03140"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNK43749.1"
                     /translation="MTENFPLYLIIMLPLLGAALNLLLGRRFGNTFVSLVATSAVAGA
                     ALVATKAVLLVASELPARGALVDQLVSGDWIVAGDFKLAAGLQLDRLSSIMVMVVTWI
                     ALLIHIYATGYMEHEPDYARFFGYLNLFTGAMLILVLGDSLPVTFIGWEGVGLASYLL
                     IGFWYQEDKNATAGRKAFIVNRVGDFGFLLGICLLFTVVGTVKYADFGSVAGRLMTPW
                     WMGWPVGYWIAILLFVGCAGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYVV
                     ARCHTIFDTFPEAAKMTVAGVGALTALMAATIGIVQRDFKKVLAYSTVSQLGFMFVGV
                     GTGAYTAGVFHLMTHAFFKAGLFLGAGSVMHAMGGEGDIVKMGGLGKHMPITRWTFFV
                     YCLAIAGIVPFAGFFSKDAILAGAFAAEFRMAGTMVPWQAKLVALYPKLLWAMLAVAA
                     MCTAFYMWRLYFMVFTGKFRGTEEQEHHLHESPLSMTLPLTVLAIGSVLAGLIGVPAV
                     MTHGLPESAEHVMTFFTQWLSPAMVPASPEPHEHWVEWALMGGALAISGVGIGLAAML
                     YRGGISQTAENLKQSLGFLHNLLWNKYYVDEIYDVLLVRPLRRIAGLLWKGVDNIVID
                     GGLTKIGPFFVDIFGAVAQRFQNGDVQRYIIAVMVGAAGILFGSTYWLAYQGIQGQVK
                     VDKRQVTLKLGDERMRPGQLIYAIDWDGDGKFEDTNVRFNSEIKHEYGSAGQYKIVVE
                     ARDPVWNMVKRSDDWFFGASVPLVAKVE"
     gene            24831..26438
                     /locus_tag="PKL17_03145"
     CDS             24831..26438
                     /locus_tag="PKL17_03145"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNK43750.1"
                     /translation="MGMILPIVTFLPLVGALLCLALPREEQQLHRSVGFGTALVTFFA
                     SLFLLSGLEPAKMNFEVDWAWVESLGIRFHLGIDGISIWLVMLTTFLMPIVFLSTWSA
                     IGDKVREFVVATLILETGMLGAFLALDLFVFYVFWEVMLIPMYFIIGIWGGDQRLKAA
                     IKFVIYTMVGSLLMLVAILYVVVKAQKVTGQWSFSLETAQKLVLTKPEQIYLFLAFAL
                     AFCIKVPLFPLHTWLPLAHVEAPTAGSVILASVLLKFGPYGLIRFAIPVFPHAVEVVA
                     PWLAILAVIGVVYGSCVAYAQDDVKKLIAYSSVAHMGFVVLGLAMLNNRSVNGALYQC
                     LAHGISTGGLFLCVGVIYERRHTRKMAEFGGLWKRMPVFAATLLIFTMASIGLPGLSG
                     FVGEFLVILGTFSAKERAFLGMPQMFENARLLSALAATGVVLGAVYMLYLYQRVMLGP
                     LTNPQNRKLQDMSARETFVMLPMLAMAFWLGICPNTFLKHIDPAVTQTLTVFKDKYAA
                     GAAESDARMLGEAAKAKVAEPAPGGNP"
     gene            26435..27985
                     /locus_tag="PKL17_03150"
     CDS             26435..27985
                     /locus_tag="PKL17_03150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNK43751.1"
                     /translation="MTFDANDLVLSLPLLCLTLAGVILLLLDAFSRVVLVGREHHLKM
                     PAETSEAVAVPPPGSRNYLMPVTVLFLLATLAIIVWQAGTLPETPVLIYRGMLQIDRF
                     GLFVSGVCVLAATLGVISAPSFLRAHRMEFGEFYALVVFSLAGMVILIQAADLVSVFL
                     GIETMSLGAYVLTGSWRRSPRSSEAAMKYFLVGAFVSGLLIYGIALIYGMTETTSLVD
                     IKRAAMSRGAAPLFYVGWMLLLVSFAFKLGVVPFHMWAPDTYEGAPTPVTGFMMSCIK
                     AAGFAGLIRLLQGTLPMLARDPFTGWTFVLSWLAVLTMSLGNLAALKQDNVKRMLAYS
                     SISHAGYLLVGVVSLGTVGEPARGPLLFYLASYALTVVASMGVVAWVGADERGEERVR
                     LADWAGLAKREPMAALLMTVSLLSLGGFPPTAGFFGKFYLFQAALGESKLLWLILVAI
                     ANSLVSVYYYLRVITAMYFRDPPPHPATTPIQSTAMRIGVLVTTLLVLGIGMFPGFLS
                     ALCAQAKF"
     gene            28100..28810
                     /locus_tag="PKL17_03155"
     CDS             28100..28810
                     /locus_tag="PKL17_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNK43752.1"
                     /translation="MKVIGAGLGRTGTMSLQAALQQLGFDPCYHMRELFDHPEHVDGW
                     MSAARGEYVDWQWFLRDYQATVDFPGCSFYKQMMVAFPDAKVLLSVRDPDRWYDSCLE
                     TIFAISHKLPMRWIGPYMPKVGPILKMASKVAWEQMFEGRFLDREFAIARYKQHNEEV
                     IRHVPADKLLVFDVKQGWEPLCKFLGVPVPEGVPFPHLNDADEFKRRIRKMQVMQTAM
                     LGGVGALLILVLRRLLRR"
     gene            complement(28807..30006)
                     /locus_tag="PKL17_03160"
     CDS             complement(28807..30006)
                     /locus_tag="PKL17_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43753.1"
                     /translation="MTSLSPDTAPPQRTFASLLVVYMTVFLDLLGFGLILPLLPFYAM
                     HFGAHGKQIGLLFACFSIAQFFGAPFLGRLSDRIGRRPVLLVCLLGSTVSLFATGLAD
                     TLYTLMAARGFAGLFGGSIATAQAYVADVTKPAERTKYMGLIGASIGMGFVFGPFVGA
                     ELSRFGFGTAAFVAAGLSAANFLLALFRLKESLPPSARGQSQRTALSVKTLREALSRP
                     VVGPLLIITTGSTLAFVSMESTFALLAKLRFGFSEQTMGRLFAVVGVMIAVVQGGLIG
                     PLARRFGEVAIGCVGFLILTIAFGIIPWMPSRELAIVALLIIAVGQGLLAPTLSTLLS
                     RAVAATEQGGTLGLSQSLGSLSRAIGPIVAGTLFDSAQFLPYLLASLLAGAMGLYLSR
                     QKGTTAA"
     gene            30119..31813
                     /locus_tag="PKL17_03165"
     CDS             30119..31813
                     /locus_tag="PKL17_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43754.1"
                     /translation="MRSVVVALFVGMLGLWGCERQTGLGSGPRHPDGSAGQGAVGFAD
                     LGGAAPDLRSVAVDLAAVASGSRTDLGVAAPDAGAAVTSGGVARLADHSDSATDTPAP
                     VLKQSAVSADLLVLELGEGDAVKKLSAIASAQAEVRCEEVSDHEAGSAHTRCDSRLVI
                     KSAPDSDSVLATFADDVPGVLCKGEETASCFESADVAISWLALPGLPGGLVMVSGSTA
                     DDHVWERENATPVAVYGLVRRGTLEPLELRELLRFSSLTHRENIREDHPEMTRRERER
                     DPMRTDEDERSDLSVMGKSKRRLPDLLATVTAETVRKFVSKPDKRQIEKHKIRYVFDG
                     IRYVPDDISLQTAAHCGSLTTHCGPLQDCCGGKCKTLSTVEHCGSCGRVCPGGLLLWS
                     DAACIDPAKGVCFFGCQGSHYDVDGKETNGCELHDTTPDLTDLGTQSCYDKPLLWQGM
                     LLSDSREHRSPALSDRDAKTGAVAKRGQVRGSGGLFCQNDLALTVTLRDGPKEPCYQL
                     AVEGLKQPLTIALSGIDDRNLVLPRGAYVSGSSLRFRLTKTCFAPAAVKFAVAFHL"
     gene            complement(31823..33145)
                     /locus_tag="PKL17_03170"
     CDS             complement(31823..33145)
                     /locus_tag="PKL17_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK43755.1"
                     /translation="MRIDLDLVSQGSDSVRRLRFDLPSIRIGRGDDCELSLPADPLLG
                     RRQCALEWHAGSLFLFPLRGSGDTVLNSVKVNRPLPLSSGDVIEVGSQIIKVFFVPPD
                     LSYPSDGPRRLTLKVTEPAAAPRLVECEQALLRICPGAHSDIYIGEPKPSRLYAQDPV
                     EAVIFWSAAGMPHLLLHKYSAVVQRNGEPIVGVTALCEGDTLTMAGTQIDVREIESPA
                     EPPRPPLGLLILDGDEPPRWHFTTRGQLSFGSAKTADVQLADPSLPSQCFSIERQKGR
                     LWLLPGGAAQTLYLNFAKVTTATAIQHGDRVSYGEVLIHIDRKTLPPTQSTVSERRVT
                     LTISDGEDEPRDVFWKDEAVRLGTTPQSEIEWPTHDDLLVDLLLLWHPDGTPRIHVGM
                     WSADAMLDGTKLRRCTLCPLHIGAKLESAGTTAIVKEIVPRPVAQPTK"
     gene            complement(33164..34465)
                     /locus_tag="PKL17_03175"
     CDS             complement(33164..34465)
                     /locus_tag="PKL17_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43756.1"
                     /translation="MKKTISVCLGLSILALSSSASAVGESTGGFPNWRERTIHEFMNR
                     SRVDPASDLAACPATACLEKSCYMPTNPLYYDLNLGRAARFHSDEMKQQNYFAHDSAC
                     TVVSNISSIYPGTCKGAASCACQGGTKACSSTCTAWSGRAPLFNTSFSGEIIATPTDP
                     KQAFYLWLYETASTNKCGYASDGSNGHRYNILMAGPSVGVGVTDAGYSVGDFGGAAAG
                     NYKIPSGSHYPQTGASIDMWANWKDSAAPSQAIVNVEGKCSTMQRKFGTATNGAYTTT
                     LTGLPSTCQRYRFEFKDSTGTTVTFPQTGSYYIGTSCATDGSDFSASPPPTCGCTPSC
                     GGKQCGDDGCGSVCGTCAANSTCNASSQCMPNSTGNPDGGGSGGNPDGGDPIVDTTMV
                     DASGCSCDVGQSRGSGAATLGSSLVLLGFATLRRRARQKSV"
     gene            complement(34498..36501)
                     /locus_tag="PKL17_03180"
     CDS             complement(34498..36501)
                     /locus_tag="PKL17_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43757.1"
                     /translation="MPRRDRKTPWASAAKTQQIVADLFVHPPVSHADLQERLGVASVG
                     DARKYLLDRMRSGKVESTDVELYGDLFHVLGLGSEADSLLRVVSDDKLDLRTRSCAMG
                     ILLSEDPSFAGKLQEVLSPDEMLQIIAQPLSDALVRVESDPAEGEGLASMMSAIPSDM
                     LVMLFEELDRWRVGSGIAASVAYEALLRSSSLSMLFPKIVEALQKQGDEDAVQLLDSI
                     RAELTDRTQQRLLQGALLRLRTRILDGQKPSPADAQGYLSGCDTLGCFVIVARRDNPD
                     ASTTLAVLCLHVSGELRSGYVLTGQSPERAARLCEEVAQESGTRFVAAPIGRLLPLVE
                     HAAEKTLQNGRSVPAEAMTALRFLSRFVSDPLKLPEPTGRLTLTALRSLFSRPEFGDG
                     WRFTQTELDRAAPPKSTTRKPKTPAVAAPLRAQPFWQQMATTLAKTDAPKKRLGSMLR
                     HMAVYYTVSGELADSKLCLLAAHDVEKDFAHSRVVQLVAENTRRWLTDMADGAIGPSA
                     PGSSPLVGADRLSWRQQLKSRFFAGIEKPSAQDLLVLDYADEALQTIVTTLSCAPSEE
                     WPRFELQQQTAFELAVAFRDQLIRQRGPSLSRLLTQSSRLVEQTMHVSRSLAGLLSSA
                     VLTRLASFASEVCAKCPVDCLGRLHADVSREFFSLEHPSRKVK"
     gene            36588..37631
                     /locus_tag="PKL17_03185"
     CDS             36588..37631
                     /locus_tag="PKL17_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43758.1"
                     /translation="MKTVKLSVLLSAVLSSVACQNGPAPAGSSLAGPETIGKTVAKLT
                     AESAALPPLLRSRWAVEYVGQVSQLLPISPRRLYFSADRRVYLSDAEARLQNNPPPPR
                     TFEMVDESAYYTGNLGSPLFHARLIDLVAEGQAAKLAGRRSLDLYAAAIGPSKLLAAA
                     GLDAVAVSASPKQRALYSQAGDQGPVRLAGRDVSGRVTFLSGDFPANPSLRAQVGTGY
                     EFVLAKNVLKRGYIRPSGEVAPDSLINLGVPDADYLRYLKETLKPGGRLLVYNLCPSP
                     NPALYDPDADCRSPWSRSEWQAAGFVVRDFERNDTEVARQFAVALGYDKPPLSLRIED
                     LYAIYTLLERPAS"
     gene            complement(37638..39188)
                     /locus_tag="PKL17_03190"
     CDS             complement(37638..39188)
                     /locus_tag="PKL17_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43759.1"
                     /translation="MFSSQRRFQLLAASLLLLSCSKNHSLTVKIITPPGSDDPFLLAS
                     QVRMTLGDQQTTAAVAGGHFSTTLTIESPTDGQQTQLLVEALDGSGMVVGRGRSPTFG
                     IQLADSEVAVYVGKPGLVTSSDLKLPDDSMQNAQGRKGLAGCALRGRRTSPPEPGLGA
                     LLVGGESEGALLSTKAWVFKHSTFQILDGGTISKGRRGAVLVPSADATIGQQGLLVGG
                     TVTGTDMITQTELFDPSVTTLSAVWSPPSVEIGDIGKPGMYRPAAVEIGDSVFLVAGG
                     AIDPNTEQPSGQAVLVKRFPAQSADMLARVGVATVPPPVDRTTALVVPRYRHSMTVLS
                     SGTLLFGGLSTSDVAAKKPVAETYAPERNIFQALTFAGTEPVSRRGHLAAKLKNGLAL
                     IIGGYSEDGSGQKTVHGSTLTVDIAARTATQQDGALKTPRWGASLHESAGELVICGGY
                     NQAGDVLSNCEFLSSDTGQQSRAPAGMPNARAEHLAIPLENDLTLLVGGVGADKKALP
                     SLDFYSIK"
     gene            complement(39209..39514)
                     /locus_tag="PKL17_03195"
     CDS             complement(39209..39514)
                     /locus_tag="PKL17_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNK43760.1"
                     /translation="MAAPFRQTADGITIDIWVMPRASRDSVGGIHGDRLKVAVSAPPV
                     DGAANDAVCIAIAKAFSVPKSRVEVIFGHKGRQKTLAVCGDQALLSAKAQALVGSGS"
     gene            complement(39516..40424)
                     /gene="proC"
                     /locus_tag="PKL17_03200"
     CDS             complement(39516..40424)
                     /gene="proC"
                     /locus_tag="PKL17_03200"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNK43761.1"
                     /translation="MTTTAAPSSDTASSPDKTLPGTIGFLGTGNMAEALIKGLLAAGL
                     VKPEQIWGSDPRQSRCQQLRQQYGINMTTHNVDVVRRAGLIVLSVKPQVLLGVMDEVG
                     PYLKPHCLCISVAAGVPLQVLQSHLPKSTRVIRVMPNTPALVGAGATAISAGNTATED
                     DIKLSQQIFGAIGFTVVLDEDQLDAVTGLSGSGPAYMFLVIEALSDAGVKVGLSRYNA
                     VALAAHTVLGSAKLLLETGQHPGHLKDMVTSPGGTAIAGLHTLEAGGLRTTLINAVEA
                     ATRRSKELGDIAIQNAEKRRQTAKES"
     gene            40520..41071
                     /locus_tag="PKL17_03205"
     CDS             40520..41071
                     /locus_tag="PKL17_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43762.1"
                     /translation="MASGEKRYAAADVREILRRAQHKDSLAVRSDSAGLSEAELLENA
                     KELGFSPQDVKTAMVAFDEDRQVGVAASELRQLAYRRLSTHAIVYLSFLAILTATGAW
                     AAAGTPLFVVAIVWAMLFLFQLRGALFPDPDKLREAARQRLLSRKLKESGKQFGTALA
                     TGAAKLLTLSAQKIDEGVKQLDK"
     gene            41125..41562
                     /locus_tag="PKL17_03210"
     CDS             41125..41562
                     /locus_tag="PKL17_03210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNK43763.1"
                     /translation="MLTVEEIQMVFEQGLPASKEWGLLVESASPEGKARVRYCFRPDM
                     IRPGGTLSGPTMMALADAAMFASILGRLGRLEMAVTTNLNINFLRRPAPADLIAESSI
                     LKLGKRLAVLEVSLFSATDDPESIEPVAHVTGTYSLPPTLVPR"
     gene            41751..42299
                     /locus_tag="PKL17_03215"
     CDS             41751..42299
                     /locus_tag="PKL17_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HNK43764.1"
                     /translation="MCGFIFGTICLVALAKVLRRGCGFRRGCGGGGCGGGGCGGGGFH
                     GYGHWQGFGPGGPGPGGEGPGFGGGRFADFVLRRVSAELDATPAQEKVLREVLDGLRD
                     SMGKNREVLRAARADVAKTLRTENFSEESLADVIARHDEVHDRMRKSLVDALAKLHTT
                     LDAGQREKLANLIANGPFFGRF"
     gene            42328..42549
                     /locus_tag="PKL17_03220"
     CDS             42328..42549
                     /locus_tag="PKL17_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43765.1"
                     /translation="MRRKLLIALLGLGVIAGYGGEIYRIRQFHKNGGAPFGRFCQQRG
                     HCCHGGQRDSAPAGPVTPAAAQTDPAATK"
     gene            42585..43274
                     /locus_tag="PKL17_03225"
     CDS             42585..43274
                     /locus_tag="PKL17_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK43766.1"
                     /translation="MASIRALLIDDDHRIAELLQAFLGQNGVSVVHQSDGQRGLQSLD
                     ADSFDVVLLDVMMPGIDGLEVCRRIRSRGSRIPIIMLTARGDEADRVVGLELGADDYL
                     AKPFSPRELLARIRALLRRTQPTSESDRLVVAGLSLDLAARRVSKDGREVDITGLEFD
                     LLLALGKRAGRVVPRDALLSLAGRDDVTVGERTVDVHISHLRAKLGDDPKSPRLIKTV
                     RGVGYVLTKES"
     gene            43278..>43997
                     /locus_tag="PKL17_03230"
     CDS             43278..>43997
                     /locus_tag="PKL17_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNK43767.1"
                     /translation="MRPHARHLQRHCAPITRRERMGHYLRSRLHRRLFLWLSAAILTT
                     VLTVIGVLRVTGNSFDWRRHQQGMAHFVVERVLSVWDRPTERRSLFHGMAHDLGVPAR
                     LLLPHGEVLDGQDFPRRCRVNFVAPLFRDRLLVGNVELCFDGGLGRSPLLRPVLALFA
                     ALLVLWTVAGLVARRIGQPLSELADVARQLGEGRLDRRAKLRHGQVGEVGELTLAINE
                     MAGRIEKQLKDQRELLAAVSHE"
BASE COUNT         7878 a        12878 c        14504 g         8737 t
ORIGIN      
        1 gctactgggc gacgcgcagc agaatcgcgg tcgtgttgtc gacaccgccg aagctgagcg
       61 cggcgcggat gagtgcatca cacgactcat caagcgtcgg tgcggtcgcg gcttccgaca
      121 tgacctcgcg caaaatccag tcatcgacca tgtcggtcag accgtcgcag caaagcagat
      181 agacatcgcc aagctgcatg ttgacggtct ggtgttcgac ttcgaccgct tcatgcagac
      241 cgacggcgcg aacgatcaca ttgtcgttgg gcgggccgta tcttcgggcc agctctgggt
      301 tgtcttcgta caggttgacc agcgagtgat cgcgagtgac ctgaatcagc tcgccggagc
      361 tatacaccaa gcggtagatt cggctgtctc cgacgtggca gacttgcagg gtgtcgccgt
      421 tttggcggat tcccaccacg gtcgtaccca tattgtgcaa agtgggatct tggcgagctt
      481 gcgtgaagat ggcgcggttg gcgctcttga tggcggtgac caggatctcg cggtcctcgg
      541 gcgggggccg ctgcccgcca cggatcactt ggtcaatcgt cttcactgcc aagttggagg
      601 caacctgccc gccaacatgg cctcccatgc catcgcacac cacgtacagc ccgatatcgg
      661 gcgcgagcag catggcatct tcgtttttct ctcttacgag ccctacgtgg gtgcggccag
      721 cttgttcaag acgcatcatg aagtattttc gacgatattc cgtaaaaagg tgccctgtca
      781 atgcgacgaa tctctctgcc ggtagcttct atgtctgcgc ctactgtggg gatttctgcg
      841 cctagcgcag gagacgggaa gttggctaca aatctgggcg tcttgtccgt gcatttcgat
      901 ggcggcaggt gccacgtccg ctgtccgttc tgttacctgg gcgagcgtga aggcactgcg
      961 cagagcggct tgccagtgga gctgctttgc acgtccttgc agacgctggc ttacgacgag
     1021 ctggcgattg cgctgtctga gccggtgacg cctgtgctgg gtccgctgcg gcgcatgatc
     1081 gatgtggcag tcgagaaaaa gcgccgggtc acgctgacca ccacgatcgc ggcggcgatt
     1141 ggcttgccgc ccgatgcgtg gcgaggggtg gaccggctga atctgtcgat tgatccgctc
     1201 aagggtcagt ttcgcgcgcc ggtagggcaa gtcgttccag aagatgtggg cgaggcgctg
     1261 cggctcttgc cgagcagcgt cgaaaaagtc ctgatcgtaa cccttgtaag ccccgagttt
     1321 gcggacacgt tggtccaggg actgttggct caccttttgg cgctgccgga agtccatcgt
     1381 gtggctctct ctgggctcaa accgccgccg ccgttttgta gccaagattt ctggctgcgg
     1441 ctgcttgcgc ggcttgggcc gcttctgcgc gatcacttgg acaagcggct gtttttggac
     1501 tgttacgtcg ccgccagaat cttgcaggtc ggcggctgtc cagccaggcc cgatctgtca
     1561 ccgaccagca aagaggacgc agcccgtgga gcgctggcgt ttcgatcctg cgtctatcag
     1621 tcgcaggcgg acttcattgc cagccgggcc gaagacctcg atatgtacct gcaagacttc
     1681 cacgttcctg cgcgctgccc gttccccatc cactagcttc attgtcgcga tcgaaacact
     1741 ttgcactgtc aggtgattgt gctgtgattc gcccaactta cctgccagga gcgttgccaa
     1801 tggatttccg actcgcttcg cgaagcttag cttcccttgc tgcgttgtgt gccagcctga
     1861 ctgtgtcgtc ggcgctggcc gccaagtgtc ccaacgtaca ctttgtgctc gatcgctcgg
     1921 gctcgatgtc aacctcggtg ccgggtgccg gtccgggcgc aacccgctgg accgtggcca
     1981 aagacgccgt caacaaggtg ctcgacaagt gggacggtca gttcccgatc ggcatgtcga
     2041 tctttccacc cacgggcggc agcggctgcg gttcggatct ggtgacggcc cctgcgtaca
     2101 gcagcaaggc gaagatcaag atggcgatcg atgccaacgg accgaacggc ggaactccgt
     2161 cgggaagtgc gatgcgcgac gcgcagatga tcaaagaact gcgcgacccc gagcgcaagc
     2221 agtacatcgt cctcatcaca gacggtggtc cggggtgcgg tggcgagccc gattcctgca
     2281 gcggcactgt cggcatgatt cgccaggcgg cgatggcaag tccggcgatc gggacgttcg
     2341 tggtcggatt cggcggtggc ctaagctcga ccgaagcgca gtgcatgacg cagatggctg
     2401 acgcgggcgg caagccagcg atgacgccgg agaaatacta caaagccgat aacgcggcgg
     2461 acctgaacac cgcgctgtcg aacattctgg aaatcatcac cggcggcggt gacgtcggaa
     2521 tgggcgggct ctgcgacgat acttgctact cgaatggctg cccgacgccg ggtgacttgt
     2581 gcgtcaaggg cgtctgcaag gccaatccgt gcgcgggcgt aagctgcggc gcaaagaact
     2641 cgtactgctc aagcgatggc tcaaactccg gcatttgcgt cagcgcttgt accaaggctt
     2701 gccccaaggg cacgcgctgc aacatgggaa cctgcgcgcc cgatccgtgc ccgtactact
     2761 gcggtgccgg tctggtctgc gacaccacgc agaagcgctg tgtgcctgac ccactttgtc
     2821 aaaacctgcc tcccgaaaag cagtgcaagg gaacgagccg ttgccgcgcg ggtgagtgcg
     2881 tcgatgatcc ttgtcgctgg acgcgctgcc cgactggcac ccgctgcgtg gcctgggaag
     2941 gcagctgcga gctgattccg accgacaacc cgccggatga caacccgaag atgccgggtg
     3001 acgtcgatgt cggttctcgc gttggtggtt gctcgacggt tcccggtggc gcaaacgcgg
     3061 tgtctatgtc cgcagccgct ctgatgctgg gtgccatggt ggcgatgcgt cgccgccgac
     3121 gtgctgagta gaccgacata agcgctgcca aagagggcga gttctggttt ggccaggact
     3181 cgcctttttt gttctttggc ctgcaaaacc tcggcaaaat aatgtggctt tagccctggc
     3241 tgtgccttgg cgtcattgac ccggaaaggg ccacgtaaga atggatcgac tgcacggcgg
     3301 aaatccactc gcagaagggc tggtcgggga cagacagccc gagccatgtg cgatggttat
     3361 ctttggcgcg tcaggtgatc tgactcgtcg caagttggtt ccggcgctgt actcgcttgc
     3421 gcgagagcgg cttttgcctt cgggcttcgc gatttgtggt tttgcgcgac ggcctttgcc
     3481 ctttgcggac gacatgaaga cggctgtcgg gcagtttgcc cggcggcgtc cagtcgatga
     3541 gtcgctgtgg gaaagcttcg ggtcggccat cagctacgtg agcggagact tcgacgatcc
     3601 ggcggcgtat gagcggctgc gctcgcacct tttggacatc gacctggccc gtggcacgcg
     3661 cggcaatcga attttctaca tttcgacgcc cccagagtcg ttcgagacga tcctgcgcaa
     3721 tctgggcaga gcgcagctca tttcgccggg cgagcgtccg tttacgcggg tgatcatcga
     3781 aaaaccgttc gggcgggatc tggcgtctgc ggagcagctc aatcgcgtct gcctcgacgt
     3841 gctgcgcgaa gaccagattt accgaatcga tcactacctg ggcaaagaga cggtgcagaa
     3901 catccttttg ttccgctttg ccaacagcat tttcgagcca ctttggaacg gcaagtacgt
     3961 cgaccatgtg cagctgacgg tggcggagtc tattggcatc gaggggcgcg gcaactacta
     4021 cgaccaagct ggaacgctgc gcgacatggt gcaaaaccac atcttccagt tcatgacgct
     4081 gatggcgatg gagccgccgg tcgcctttga atcgggcgcc gttcacgatg aaaaggtgaa
     4141 agtgctgcgt gcactgcgtg aagtgccgct cggtgaggtt gatcggttta cggtgcgggg
     4201 ccagtaccgc gatggtttcc accaaggcca gccagtgccg ggctatctgg ccgaacaagg
     4261 ggtatcgccc acgtcgcaca ccgagacata cgtcgcgctc aagctgttta tcgacaactt
     4321 ccgctggggc ggggtgccat tttatgtccg ggcgggcaag cggatggcgc gacgggtcag
     4381 tgaaattgcc atcacgttta agcagcttcc gcacacgctg ttcgcggggc cgcaaggcaa
     4441 gctctcgcat gagccgaacg tgctgtcact gcgcatccag cccgacgaag gcattgtgct
     4501 caagttcttg tcgaaagtgc cggggccgac gattgagccg cgcccggtca cgatggactt
     4561 tcgctatggc acaagctttg gcaccgaacc gccggaagct tacgagcgct tgcttctcga
     4621 ctgcatgctg ggtgacagca cgctctttgc gcgcggcgat gaagtgatgg cgtcgtggcg
     4681 gtactgcgac cggatcttgc ggggctgggc cgagcaggac gcacgcaacc gggcaccgct
     4741 gccaagctac gagccaggcg catgggggcc cgagcctgcg gcgaccctgc tggcgcgtga
     4801 tggccgggtg tggcgacgac catgagcgaa aacggcgact accggcaaaa cgaacgacgc
     4861 gagggctggg cggtgccgct ggatgcggtt tctgcgttta cccaaggggc atccgtcagc
     4921 gtcgcgcccg atcgcatcga acaagagctg cttttgctgt ggcgcaaggc gagtgagcgc
     4981 gcgcaggcca agggcgcggc ctttgcggtc acgcgggctt gtcagtggaa cttgatagtc
     5041 cacacccatg gcgaggaaga gcttttgacg atgaaacagg tgctcgatac ggtgtccgag
     5101 actgtgccgt gtcgggcgct ggttttgcac gaaagcgatg catcgacgtt ttcgggcgtg
     5161 attggtgacg acggcgccgc gatgcatgcg tcggtcgaag cgaacttccg cgacagcccg
     5221 agcggtcggc gcgagattgt ggcagaagaa atcaccatcg agacgccgca gcttcattcg
     5281 cgccggttgc cgagcttggt tcgctcgctg ctcttgccgg atgtgccgac gactgtcttt
     5341 gtccacaatc ctgcgctcga tgccgattgg ctgcgtcccc tgtgtgccga tgccgatcga
     5401 ctcattttcg atagcggtcg gctgcgcagc ggtggcgagc ttggtcagct tgcccaggtc
     5461 ttgccggagc tttggccagg ccacgagatg caagtcgagc tgatggactt gggctggctt
     5521 cggctgtggc cgtggcggct ccttttgtca tcgctttacg acacgccaca atcgcgcgca
     5581 ggtcttgcac agctccgcga gatcgaaatc ggtcacacgg cagggtttat ggcgcaagcg
     5641 ttgcttgtcg ccggctggct gctcgggcgg ctcgggcttg tgcagacggg cacgctcgaa
     5701 tcggacggca gtgttggact tttgccgggt gctggcagct cggtccagtg tcccgtgcgg
     5761 ctgcggctgc ggcagtgtga ggttgcttgc cgggcaacgg gaatttcgca tgtcaccctg
     5821 cggcaagcgg actacgaact gtcggcgcaa aatgacgcat gcggcggggg aatcgagctg
     5881 cgcagcccgt tccagccggc ccggacccag ccactgcgcg gtcgacctga tgcagagctt
     5941 gtcatcgccg cattaggggt tggcgggcgc gatccattga tgtatgaagc gctgcaactc
     6001 ggggcgagcc tgctcctggg cgatgccatg ttgagagcag agaagcagct agaggtgcga
     6061 tggtgagtgg acgagcgccc gagatttgga tcggcgaaga cggcgaggct ctggctcgtg
     6121 agggcgtgcg gcgctttgtc gacgtagctc ggcggtcgat tgcggcacgc ggtcgcttcg
     6181 tggtggcact ggctggcggc aatacgccgc ggcgtctgta tcagcttttg tcgcacgtcg
     6241 attcgatcga ctggggtcgc gtccatgtct gcttttccga cgaacggatg gtggccgctg
     6301 acagttcgga cagcaacttc aagatggcgc acgacagcct gcttggcgtt gtgtcgattc
     6361 cgcccggaca gatccacgcg attccgacgg cgatgtcggt ggatgaagcg gcgctgcgct
     6421 acgaccagac gctgcgcgag attctgccca aaaacgagcc acggcttgac ctggcgctct
     6481 tgggaatggg tgcagatggt cacacggcgt cgatctttcc gggatcgcgg ctgctggccg
     6541 gggtagacga tgactttctg ctcgacgatc ccgaaaccgg gccgctgcgc aacgacgttc
     6601 cgacgccatc gcttgacagc ttcagcagcg agcggctgtg tgcgccgatt tacgatgccc
     6661 caaagccgcc gcgagagcgc gtgacgatga cgccgtatgt gctaaacctc gcgcggcagg
     6721 tcatcgtgct ggtggcgggt gatgacaaag tcgcggcggt gtcggcggcg ctggaaagtg
     6781 atgcgcgggt cgccgagata ccgattcgcg cgattgcacc tgcgacgggc gaacttgtat
     6841 ggctgctcga tcgcaaagca gggcgtgcgc tcggcaggcc gatgcgttaa gacggcagca
     6901 ccgtttgtct ggagctttgc ttgcccatgt ctaccgctcg ctgtctttgc ctgcttggac
     6961 tgattctggc ttcgtcggca tgcacggaaa aaatgccgaa gcttctgccg cccgtgtttt
     7021 ccgacaactt cgatcgcacc gagcttgggc caagctggaa gtcgacggca ccgcctggca
     7081 cgtatcggat tgaaaacggc gagctggtgg tatcgggtgg caagaaccat ccgctgtggc
     7141 ttgttccggc gctgccgcgt gatgcggtga tcgagctgga tgcctatagc aaaagtcccg
     7201 atggcgacat caaggtcgaa gcgtttggcg acggacagag ctttgccaag acggtgtcat
     7261 acaccgcgac ttcttacgtg ttcatccagg gcggctggca aaatcgtctg gcggcgctgt
     7321 gtcgaatgga tgaacacgcc gacgatcgca agacccgtgg cgatttcaaa gtcgtacctg
     7381 ggcagcgcta tcactggctg atcgcgcgca aagaccagct ggttcgcttc tatattgacg
     7441 gcaagcttgc gctggaaatg gatgacttgg tgccgctcga agggccgggc cacagcttct
     7501 ttgggtttaa caactgggaa gcggaagtcc atttcgacaa cttggtcgtg cgaccgtact
     7561 gattcgggcg ctggatcggg cttgcgaagc tctttgggga tcggctgggc agcttaggcc
     7621 agcgtgatca gcatcggacg cgagccacgg gcggcgagga cgagcgcttt gccatcggga
     7681 acggcgtttg gctcgccgaa aaccggtgtg ctgccgactt ctgcgactgc gagcgtgacc
     7741 ttctgaccct gcgccggggt ctgcccttcg ctggtcactt ggtagtgcaa gctgctctgg
     7801 ccgcgacggg cgatgaccag catgtcacca tggcttagtg cggcgacgac acctgtgccg
     7861 gcttcgtcgg gaactgcttg gcgcagcatc ttggcgagca cggcgacggc ggcttcggca
     7921 gacagctcgg cagtggtcag gtacgccgga tctgcatcgt gcaggttgcc aagcaggccg
     7981 aggaaaaaca gctcggcttc actgcgaccg cgcaggttgt cccgcagcga aacgggcagg
     8041 tcggcggtgt gatgggcgcg tgctgctgtc ggctgaagct gacccacgac cgcgcagaga
     8101 aactgccggt aacggaagac gccttgctga agcggcggtt cgccaatcac cgacataagc
     8161 tggtcggttc gcaggttggc ggtggcgcga taggcggaca gttcttcgct cttgccgcgc
     8221 tgaatcaggg gttcgccgtc gatgtaaaag ccctggtcgt gtgaaacggt cttgccagga
     8281 gcggcgggcg cgtcatcacc ggctggcacg gcgtcaacga gcgcggcttg cggcacaaac
     8341 ccggctacac gctgaaaaag cccgcgcgca cgcacgggtt cactgctacg gaggactagg
     8401 agtcgggaca tgagcttctc gtattcacgt tccacgatac ggcagcacgg acagttccga
     8461 caagagcggt gttgggccgc gagcaagcac tgccttgaca gcgctggcat cgcaggtgaa
     8521 tgatgcaagt agatgttgaa aacgattttc aatattcgct tgctgttcct gatcggtctt
     8581 ttgtggccgc ttgcagggca tgcttcggat gacgaacgcg cgatgcagac ccagcaggtg
     8641 gccgcgcttg tcgactatgt ggctgcggac tatgccgggg cggtcaaaga cggccaggtc
     8701 acagacgaaa gcgagtatgc cgagcagcgc aatctcctcg aagaagcgag caagctggca
     8761 caaaagctgg gcggcagtga tgcagaccag gcgcggcttt ctgcggcgct cgcagaggtg
     8821 cggtctgccg tttcagccaa gcagccagcg acgcttgtgg gcgagcgctg tcacggggtg
     8881 cggcagatcc tcaaggacgc ctatcagctc aagatggtgc cgtcgagcaa agtctcttcc
     8941 agtcaagcgg cggcccagtt tcagatgttg tgcgcgtcct gtcatggagt gacgggtcgg
     9001 gccgataccg aagccgcgcg caagctgacg ccgccgcccg tgtcgttttt cgacgaagaa
     9061 aaaatgcccg gtgtggcgcc gcagcttgcg tttcatgcgc tgacctttgg gctcaatggg
     9121 acggggatgc cgagctttgg ctcgctgccg gcgcagcagc gctggaacct ggctttttac
     9181 gtcgttgggt tgcgtcacgg gacgccgggg caggacaagc cgtcggcgct gtcgtcgcac
     9241 caagcggtcg cggcgctgtc gctcgcggat ctggcagaac agtcggacaa ggagctgtgt
     9301 gaaacgctgc agaagggcgg actcgacaag agccgctgtg gcgaagcgct ttcggttttg
     9361 cgaacccaga cgccgtttgt gtcgattgtg gaaggcccga tgcacttcga aaaagcacgg
     9421 gcgctgcttg tcgacttgcg tggcgcggtg gagcgcgggg atggggcatt ggcacagcgg
     9481 ctggcgattt ccgcctatct ggacggtgtc gagccaaacg aagcttcgct gcggattgag
     9541 cagccggagc ttctgcgcaa aatcgagtcg acctatctgt cgctgcgcca gtccgttgat
     9601 ccgaagagcg gcgtcagcaa agaagcggtg ctggccaaaa tcgtggaagc gcagaacctg
     9661 ctggccgaag cggagcgtgg cagtggtggc acggtggcgg cgtttctggc ctcgctgacg
     9721 attgcgctgc gcgaggggct ggaagtggcg ctgctgattg cggcgctgct cgcgtttttg
     9781 cgcaaaagcg gccaaggtca cttggcgcgg ctggttcaca tgggctggat gctggcggtg
     9841 ccggcgggct ttctgacgtt cgttgcggtc ggggctttgg tgtcgggggc ccagcgcgag
     9901 ctggcagaag gcatgatgac gctgcttgcg gcggcaatct tgatcacgat gacgcactgg
     9961 atcatcggtg ccaaagaagc gcggcactgg ctgggctttt tgcgtcgcag agtcgagggt
    10021 gccggcgaac aggccgcgct ttcgcagaag ctggctcttt tgggtctgtc gttttttgcg
    10081 gtctatcgcg aggcggtgga agttgtgctg ttctatcgct cgctgctggt cagtgcgggg
    10141 ccgcaaggtc gttccgatgt gatctggggc gcggtggtgg gtgtggtggt gatgtttgca
    10201 gtggtgctta tcgtcggtcg gattgggcgc aagctcaatc cgcgtccggt catgttggtc
    10261 agcagtgcgc tgctggcgct gttggcactg gtgatgacgg gcaatggcgt gcatgcgctg
    10321 caagaaggcg gctacgtcgg ggcgacgctg attcacgcat ttggcagaga gtggggtggc
    10381 gtgccggtcc tggggcttca tccaacgtgg caagtggtgc tttcgcagtg tgtggtggcg
    10441 ctgcttttgg tcgtgccgtc tctgattgac cgcagtcgtc gtcgcgatgg cgatgcgccg
    10501 ctgccgccgg ttggtcactt gcccagcaag gcgtaggcgg gcgaagccac gactgcaacg
    10561 ccactgcaaa cggttcatgc atccgcctgt gaacagtgtg ttacggctgg ctgggcgggc
    10621 tttggcgctt gcgtagcttg gcgacgatgc tggtgccgat gagacggtct tcgtcggtaa
    10681 cgatttcgag catcgcacgc agcagcaggg gactgcgaaa gcacagcgtc agctcgtcat
    10741 ctacttgggt gggccgaatc cattcgagcc actcgtggtc gatgagcatc gccaaggttc
    10801 ggtcgagcag cgacagcgaa aaatccgtcg gcaagagctg tagaagctcg ccttgggtga
    10861 agcgatcggg caatagtgcc gcggcgcgca acacctgctt ggcctgggga tcgagccgat
    10921 gagcccgcga catcatcatc gcgatgaccg cccgtgtgcc gatggtctgc ccggatgttt
    10981 catccttggc cagccacagc aaatacagtg ggagaccgca gccggcgtgc gccaactcct
    11041 cgcggcgctg ggaattttcg atgcccaggt ggcacagcag cttgtctgcc gacttgccgg
    11101 gcagccgacc catgcgcaca cggtcgatgg gctggccttc gagaaagcgg ggcagcagct
    11161 cgtcgatttc cggacgaccc aggccgagca aaagaatcgg cgtatcgaca agtcgcaaaa
    11221 acaccgtgga aagcgcgctg cgtgatgccg catcaatcca gtgaacgttg tcgacgacca
    11281 acagcagcgg gcgacccact gcggctgcga caaacagcgc cgccagcgca tcggccaccg
    11341 aatcggcacc cggcaaggtc agcggtgtcg cgctcggcgg tctgggcagc gcgtggtgca
    11401 gcagtgccga caggacatgc tgatgctgcg cggcttcgtc gtgcggatgg cggtcaaaca
    11461 gccacttttg ccaaagcgga cgctcgctgt gatcatcgag ggacttttgg tcgacgctcc
    11521 gcagccaacc gatcagcgcc gaaaacgggg cgcttcgatt ttggggtgca agacggaccg
    11581 acaaatgggc gaagtcttca ccggcatcgc gcattcgtcc gataagttct tcataaagcc
    11641 gggtcttgcc gctgcctggc gcaccgagca ccagcaccgc acgcgacact tcgtcttcgc
    11701 ttgcgccgtg aatcgctgaa tgcaaaagcg acagctcgcg atccagaccg atgagcggca
    11761 gccggtttgt tcgcagcacg gcttcactga gccgggcgcc caaatggaaa tatccgccag
    11821 gagcagcact gacgaggtag cggtcgtgga gaagctcggc agatagctcg tcgagccaga
    11881 tcgcgccatg gctgtcgtgg tccagcgcgg cttgcaggat tcgctgcgat tgttcaaagc
    11941 cggggcctgt cgaaaaaagt ccatgccgct tgcacattcc cgtcgaaatc acgacaggcc
    12001 agtgcacaag ttcgctgcgc aagatgctcg ccgtgcgggc ggcaagctgc acctgatcgg
    12061 tggcggcgaa ggcatcgaca atcgttgcag acagcgagcc gtcagcgagc agcgcggctt
    12121 ccacaccgat ggctgccaaa tgatcgagca cttggtgccg cgccatacgc agggaggccg
    12181 cttggctgtc gtcgagcgtt gtgattcgtc tctgctcact cttcggcgcg gtggccaata
    12241 ccagcgtcgt cagcagcagc tcgtctcctg aaatggctgc gatctggact ggcggcggtc
    12301 tgggttgcga gtcgccggct gtaaactcga tttggcacag tcgctcataa acgtcggctg
    12361 cactctgggg tcggtgtagc ggatcttttt cgagcagctc ggccaccagc tcatcaagcg
    12421 gctggggcag atccggtcgg aaggtgtgca gtcgtggcgc cggttcaaac aggatcttgg
    12481 tcaaaagacc cgtgatgtgc tgcgcagcaa acggctgctg gccggtcaag agctcgaatg
    12541 cgacacagcc gagcgcaaac agatccgccg aataggtgat gtcgcgccga ccgcgggctt
    12601 gttcgggcgc catgtattcg ggcgtaccga cgacaactcc ggttcgggtc agccgggtca
    12661 gttggattcc gcggcgggcc accccaaagt cgagcaccgt cgtttcttcg ggccgaccgt
    12721 gacacagaaa cagattggag ggctttacgt cacgatggat gatgccgttt tggtgggcga
    12781 cggacagcac ttcggcgacg cggcgcaaaa ggattccggc ttcggcagtc gaaagcggct
    12841 tttgcaaaag ccgccctgcc aggtcttgcc cttccagcca ttccatcgcc aaaaacgctt
    12901 gggtgtcggc ttggccatgt gcgacgtagc ggacgatgcc ggggtggcga agctcggcaa
    12961 ggagcccgac ttcgcgttca aaccgcgcca cttcttcggg ctggctgaca tcaaggttga
    13021 gcagcttgac ggcaacgagc tgtttggtcg acaggtcgcg ggctttccac accgtaccca
    13081 ttccaccttc tccgacttgc gacagaagct caaagcggcc cgcgagcggt ttggagagga
    13141 gctgggacat gcggcgtggc tagcgatcga ccgagcgatc atacgagagc ttggcgcaga
    13201 aagaaatcta ctaggacgcg acgggcgatg acgctatctt cgcggcgccg ggcgacggca
    13261 aagacggacc ggcgaacggc aaaacgacca tggcggtgtg tgtaaggacg agtgttccag
    13321 ggccgaagtc gcaagccttg cttttggcag aacaaaacta tctggcgccg gggattcagc
    13381 agatatcgac gctggcgggg attgccgtgg ctggcggtga aggcgcgctc atcgaagatg
    13441 tcgacggcaa tcgctttctc gactgggtgg ccggcatcgg ggtggcgagc atcggccacg
    13501 gtcacaaggc gctggcccag gcgctttcgc agcaagccgc gagcattgtt gcgaccagct
    13561 ttgcgtcgcc ggcccgactg gaactgctcg cgcgcatcgt cgagcatgcg ccacatccca
    13621 gcttaaaccg tgtgcagcta tattcatcgg gggccgaagc cgtcgagagt gcgctgcgac
    13681 ttgcgcgcgc cgcgaccgga cacagcgaag tcttggcatt ctggggtggc ttccacggca
    13741 aaacgctcgg ggtgcttggg cttatgggca gcgagtggaa gcatgggctt ttgccggttg
    13801 tgccgggaca acacctggcc ccgtatggcg actgccgaag ctgcgccctg cgcttgtccc
    13861 cggacagttg ccgcatggcg tgcattgact ttttgcgcga tcagattcgg ctttcgacgg
    13921 ccaatcggtt ggcggcgatt gtcgtcgagc cgattcaggg caccgcgggc aacgtcgtgc
    13981 cgcatccgtc ctttttgccg gcacttgccc aggttgccca cgagcatggt gcgctactgg
    14041 tcgtcgacga aatgatcacg gggtttggcc gaaccggcga cctgtgggcc agcaccgctg
    14101 cgggcgtcgt ccccgacatt gtgacgtttg gcaaaggtgt cgccgccggc tttccgctga
    14161 gcggccttat cacccgggcc gagcttgctg cgtcccgtcc gtgggctgac ccatcgcatt
    14221 cgtcatcgtc gtttggcggc aacccgctgg cttgtgcggc agccaatgcc accacgcgga
    14281 tcatcatcga cgaaaacctg agcgaaaatg cgcggcgggt cggcgagcgg ctctttgcca
    14341 aactgaccga gctgcatgcg cgcttcggct gcttgtcggg actgcgcggg cggggactga
    14401 tgctgggatt tgaccttgta tcggacggca agctctggcc caaggatcgc tgtctggcgc
    14461 tgtttcgcgc ctgtctttcg cgtgggctct tgacgatggc ttacacccca cgggtgcgaa
    14521 tcaacccgcc actggtgctg tcgcttgcgc aagccgacga gtcgatccat atctttgccg
    14581 acgcgctggc atccgtcgcg tagctagcct gtgccgggcc cgacgcgact tatcaccaac
    14641 agcggaagct ggggcggcag ttccgaatag gccaacacgg gtaacgcctg cggcgtggca
    14701 gccagcagcg actcgacgtg ccgtcgcaga tccttttggc aaagcagcac ggcgtaaggc
    14761 gcacgggtct tcgcggtcag cacggcgtcg atgatttcct gacggagatc gggctccaaa
    14821 gcaagccggg tgccgccacc gctgcgtcgc gcatcgcgca gcacgtcttc gacatcgctg
    14881 gacagaacca gcactggcag accattttcg ccgtcgctgc gctcgtttcg ccaaaagccg
    14941 ctgagcagat agggtccaag ccccgagcgg acttgctcga tcaagccgtc ggcgtccgca
    15001 tcgagctccg gcagggtcga aatggtttcc agcaccgcag cgatgggcaa cggccagacc
    15061 cgctcgctga gcagtcgtcg caagatccag agcagccgac cgagcggcag ccgcttgggc
    15121 acaacctcgg aaaccagcgc cggtcgctcg ctgccgattt tggcaatctc cttgcgcagc
    15181 ccgtcgagcg acaggagctg cgccgccaag tcgtacagca tcgtttgaag cactgcgtcg
    15241 ggccgctcgt tcgaaaggac cgtgcgctgg gcgagcagcg cgccgtggat catcacgcgg
    15301 aattgggttc cggtaagcgg ggcaaactcg atgacgaagc cgacggagcc aaggccgata
    15361 tgtgccaaaa gacgctcgac gaccgacaca agctgggctt cgctgtgttg aatcgctgcg
    15421 ctcgcgatca cgcgcagggt tggtcccatc gtggccccgt ctgatggggc ggtggcactg
    15481 gtgctgcgca aaagcaacag caccgctgcc gtcgtcacca gcatgaccgg tagctgcgtc
    15541 gccagtccga ccccgatgac caagcgggcg gcctgctggg ctgcctcgga cgtgggcagc
    15601 tcgctggtct gcgaaagcga caccatctgc gcgacgccga tgccgagcca caacagcacc
    15661 gaaagcagca cgtcgccgcg cagaagccgc agcacaccgt ccaaagagcc gtacagctcg
    15721 gcttcgcggg tcagctcggc acgggcggcc tgggcttcga cgacaccgag cgcgccctga
    15781 cgaacgtcgg catcaatcgc ggcttggcga cccggcatgg catcgagcac aaagcgcgcc
    15841 gcgacttcag cgacacgctc gccgccgcgg gcgagcagca cgtactgcgc gacaagcagc
    15901 gcgaacaaaa ggagcagtcc gatgctgact tccgacgcgc ccatggtcga ctgcagcagc
    15961 gccgcaagca gaccgggctg accgctcgcc acggcttgcc ggcccagcgc cagcgtcagg
    16021 cacagccgtc cgagcagcgt caagagaagc agcgtcggca gctgtggcag ccgcagcggt
    16081 tcccctgccc acactgccgc gatgagccac aacgtcgccg acagccagct tgtgaacaaa
    16141 agcccgtcga gcagcagccg gggcagcggc agaagcagcg ccagcagaag tgatatcacc
    16201 acggtcagta gcaggcgcgg cgacagcttt tggcttggct ttagccactt ggccaaaaaa
    16261 gcaaacagtt cgctcagctt ctgctgcatt ggttccatct tgcggtgtgc ttctggggcc
    16321 tgtcaacgac gggacgaacg cttgtgacgc aggtggtgtc agcggatagc caggaccatc
    16381 gaaagtacga taagatgtca gcccaaatac agttgaagcg gcacatgggc agcttttcgc
    16441 tgagctatag caccgtcaag agctgggccg agcggctcaa gctgccgttt cgtgccagcg
    16501 acgaagcggg gcagattgcg gtgcactgtc ggctgctgga cagcgacgtg ccgctcctgt
    16561 tcattcccag gccagagcgc gacatgctga cggtggcggt gacgttgccg tttgtggtca
    16621 agacggaacg tttcgcggcg gtgacggcag cgctgtcgac gctgaacgtc cgctcgtata
    16681 tgggggcgtg gattttgaat cccgagcggg gcgaggtcta tttccgcgtc acgctgccga
    16741 cgctggacac ggcattttcc gacgatgcgc tgcgctttgt cgcccgcgtg gtgttaagtt
    16801 ctgccgaagc gatggcgcaa cctttgtatt cgattgcgca cgaaggcgct tcgccagaaa
    16861 ttgtcgccaa gacgcgctct gcgacgtaaa cgctcgctgg cgtttgccta aaacgcgctg
    16921 atgcgaacag gcaggcgacg acgaccaaaa tcaccggctt tggcggcgaa atggccggcg
    16981 agtttctgtc cgcagcgtgg gcagccagcg cttgggtcca ggcgatacga caggatctga
    17041 tagtagtcgc ggacaatcag gctctcgccg cagcctgcgc agatggtggt gtcgccttcg
    17101 acgtcgtgga cgttgccggt gtaaacgtgc tttaggccaa cgcgacgggc ggtggcgcgg
    17161 gcgcgctgga gcgtttcagg gcgcgtcggc ggcacattca tcatcttgta gtcggggtgg
    17221 aacgccgaaa agtgcagcgg cacgtcgggg cgcagcttgg tcgcgaccca ttcggacaag
    17281 cgggtgattt cttcgtcgga atcattgaaa ccagggatca atagcgtcgt aatttcggtc
    17341 cacaccgacg tcttttgcac caagtattcc agcgtctcta gcaccggctg aaggtgcgac
    17401 gcggtaagcc gcatatagaa ctgttcgcta aatcccttga ggtcgacgtt ggcggcgtcc
    17461 attttggcga agaaatcggc gcgcggttct ttgtggatgt aacccgcggt gacagcgacg
    17521 gtctttatcc cggcttgttt gcaggcgtcg gcggtgtcca tcgcatactc ggcgaagatc
    17581 accggatcgt tgtaggtaaa tgcgacgctc atcgcgcctt tggcgacggc ggcgcgagcg
    17641 atttgatcgg gactggcctg gtctgacagg cgatccatct ctttcgattt ggaaatgtcc
    17701 cagttttggc agaacttgca cgacaggttg cagccagccg taccaaacga cagcaccgac
    17761 gaaccgggat agaagtgggc gagcggcttt ttttcgattg ggtcgacaca aaaccccgac
    17821 gagcgcccgt aggtggtcag gatcatgtgc ccgcccaggt tttggcggac gaagcacagg
    17881 ccgcgctggc catcggcgag cttgcagtcg cgcgggcaca gatcgcactg aattctgccg
    17941 tcaggcaagc tgtggaagta gcgcgcgggg tggctcgact cggtgatggt catggggtcg
    18001 tgggctcctc gatccacttg tcgacggtgt agcggtagac ctcaagctcg gggctgtcga
    18061 aatcagtcgg caggccggcc ttgcgcagaa ggtgtcgcca gaattcacgc ggctcgggca
    18121 gctcgtccca gacctggggc aaaaacgtcc cgcgtcgtcc atgatatcgc aaaagcacgc
    18181 cgtctttgtg gggtcgcagg gcggcaatgg cttcggcgcg gcttttcacc ggcagacgca
    18241 caagcggcga caaaagcgac acttcgacgc gaaactgctg ttcgtcaccg agcccaatcg
    18301 gcatcgcacg cggatctcgg agcgccgcgg ccaccgcatt ttccgccaca tcggcgacca
    18361 gtggctggtg ggcttccaga ctgccgatgc agccgtgcag ctctccgtct cggtacagcg
    18421 tgacaaagca ggccccgtct ttatcaaacg ccggattttg cggcggcacc gcgtcagggc
    18481 cacccagggc ggcgcgaatc gacgcacggg catattggca gagcgcaagg ccttcagctt
    18541 gacttggcat ggctgtcctc atagacggca aatgcgccgt aaccgacgac atgggctttg
    18601 tcgccggcgg tgtcgcccga gctgcgcaga tccagcagtc ggactttgag cttccgacgc
    18661 cgcacgaccc acaaaagacc gttaatgccc acacagccgc aggcttgttc gggggtaagc
    18721 acgtcggatt ccgctggtgg cgacacgatc aagtcggcgg tgtgacggtc ttcgctttgt
    18781 ccttcgaggt agggcaggta gtgcgacagg tcgctgctta cgatgatgac cgtttcgggc
    18841 ccgccccaca gcgaatcaag gacttcgccc acgtcggggg ctgatgccca gccggcgacg
    18901 agcggcacca ctttggcgtg gggcacgacc ttctgcagaa acggcaactg cacttccagc
    18961 gagtgttctt gcgcatgcgc acgcggattg gccagcacgc ttggcaggtg ccgtagcgtt
    19021 tcgatgtcca ccgcgacatc gccgagcggg gtgcgcagcg ctgatgctcc cggtgaggtc
    19081 agcccgtgga cgggaacccg gtgcgctgga cctatcagca ccactcgttc gatgtggtgg
    19141 gcgccttgga gctgggcata cgcactggcg gcaatcggtc ccgaatagac gtagccggcg
    19201 tgcggcacga tgagcgcttt gggaaacggc ggcctgggcg tcgcattcgc gagcaggtgc
    19261 gacaccgtat cagcgagaat gtgcgcttcg cggggataga acagccccgc cacggcaggc
    19321 ggacgaatcg cggtctgcat acagataact tacgagcttg gaggcacgag gcaacccccg
    19381 gcggaatccg gccaggccta cgcggcttgc gcgacttgct ggccacaaac ggccagcgcc
    19441 aaaggcagcg cccgtcggaa aaccatgtag tggctatgcg tttcgcacgg attgacactc
    19501 agacgaaact ccagtaccgt tgccgcgctt gccgagataa ctgctatagg caatccccgg
    19561 aaggtgatat ggcgctgcaa ttctcccctc aggccgacgc caaaatcagc gacatcacac
    19621 gtcgctaccc caacactcag gccgcttgcc tgcccgtcct tcatttggcc caggacgagt
    19681 ttggatatct gtcggaggaa gtgatcgaac tggtggcagc gcggctgtcg attcccagct
    19741 cgcacgtctt cggcgtcgcg accttttaca cgatgttcca ccgcgagccg gtcgggaaga
    19801 aggtgctcat gatgtgcacc aacgtttcct gcatgctcac cggcgcatac gagacgctgc
    19861 gcaagctgga gcagcggctc ggcatcaaag ctgggcagac ctctgcggac ggtgagttca
    19921 cgctgatcga ggaagagtgc ctggcggctt gtgccgacgg cccggccatg atctgcggcg
    19981 accgctactt cctccgtctg acggcggaca aagtcgatgc gacgcttgat gagtgtcgca
    20041 agctgcccgc gcagcatggt caccacccac acagcgacaa aaagcactaa accatgccgg
    20101 ctgatcgcaa caccaaagtc gttacccgtc tgtttggagt gcaaaactcc aatcgcattg
    20161 acgtcgcgat ggcaaacggc gcctacaagc ggctgccacc tgcattcgcg cagcagccga
    20221 atgccatcac cgaagaggtc aaaaagagca acctgcgggg tcgtggcggc gcgggcttcc
    20281 cgacgggcat gaagtggggc ttcattccca agggtgcgga caccgtgtac ctcgtctgca
    20341 acgctgacga gtcggagccc ggcacctgca aagaccgcga gcttctgttt tgggacccgc
    20401 atctgctcat cgagggcatg atcatcgcgt cctacgcgct cggctgtcgt cacgcctaca
    20461 tctacattcg gggcgagatg attcgtgaag cggcggtgct gcaaaaagcc gtcgatgaag
    20521 cgtatgccaa aggcttcctt gggcgcgatc agcagacggc gattggcccg gtgaagtttg
    20581 acctgaccgt gcaccgtggt gccggcgctt acatctgtgg cgaagagacg gcactgctca
    20641 actcgcttga aggcaagcgg ggctggccgc gactcaagcc gccgttcccc gccgtcaagg
    20701 gtctgtttgg caagccgacc atcgtcaaca acgtcgaaac gctgatgaac gtgccctaca
    20761 tcatcgagca cggcagccag aagttcgccg agctgggcgt cggcaagtcg ggcggcacgc
    20821 gcatgctgtg catgagcggt cacttaaagc gccccggcgt cttcgagctg cccatcggca
    20881 tcacgttccg ccaggtcatc gacgaggtct gcggcggcac ccacagtgga aagcgcatca
    20941 aggccgtcat ccccggcggt tcgtcgatgc ccccgctcga tgcgagtgag ctggacgtgc
    21001 cgattgagtt tgacgcactg atgaacgacc cgcgcatcaa gccggtgcag tacaaacccg
    21061 gtcacaactt cgagctggcg ccgggcaagc cgctgcgcgc gatggctgga tcgggcggca
    21121 tcgtcgtgat ggacgaagac accgacatcg tttcggtgtg cgcgcgcatc atgcgctttt
    21181 acgcccacga gtcgtgtggt cagtgcacgc cgtgtcgcga aggcactggc tggatgtcga
    21241 aaatctgcac gcgactggcc gatggatacg ggcagccggg tgacatcgac ttgctcagct
    21301 cgattgccta cggcattgcc ggcaacacga tctgcccgct cggtgaagcg gcagcgtggc
    21361 cgatgatggg ctttatcacc aagttccgtc ctgacttcga ggcacgcctg gcccaaagtc
    21421 gcggacttcc ccctgctggc tatcacggag cgcatccatg attgcactcg cacagaccga
    21481 ccttgcctcg ctgcttatgc aggcggccca ggtgcaggcg gtgccagcct cgtcgctgtc
    21541 gccgactggc tggaccccgg catgggcgtt ttggattttg tcggcgctgg tggtgatcgg
    21601 ggccatctcg accattgccc agcgcaacct gatttctgcc gtgatgtcgc tggtcgcgac
    21661 gttctttgga cttgcggggc tttacgccac cctgtcggcg cactttctgg cggcgattca
    21721 agtcctcgtc tatgccggcg cgatcatggt cttgttcgtt ttcgtcatca tggtgctaaa
    21781 ccgcgacgaa gccgaacggg tgtcggatcg cgggctgctc gacaaagccg tgggcgctgg
    21841 cgcgctggtg tacctggcgg tgcggctcgg cgaggtgatt gcgtcgccgg tcggtccggt
    21901 gtcgcggatg agcaagccgc ccgcggactt tggcaccgtc gccaagatcg gcgagtactt
    21961 cttcaccgag ttcctgtttc ccttcgaagc catttcgatt ctgctcatca tcgcggtgat
    22021 tggcgcggtg gtggtggctc gtaccgtggt gtcgcgtccg acgaccgggt acgaagtgcc
    22081 gaccgctgcc gagcacgctg gcgagggcgg agccgaggac gctgccggtg gcgattccca
    22141 cggcggacac ggaggccact aaagccatgc agctcatcct ccagaacctt ttagcggttt
    22201 ccctgcacca ctacctgatc ttggcggtgg tgctgtttgg gatcggcaca ttcggcgtgt
    22261 tcatgcggcg caacgcgctg acgacactga tgtcgattga gctgatccta aactctgcca
    22321 acctgacgct cttggcgttt gcgcggtcac accaagacat gcgcggccaa gcgctggcgc
    22381 tcctggtcat tgcggttgcg gcggcagagg cagcgattgg cctcgccatc gtggtcggcg
    22441 tgttcaggaa ccgtcacaac accaacatcg acgagctgaa cttgctccgg tactagtccc
    22501 caggaacaac gactatgacc gagaactttc ctctctatct catcatcatg ctgccgctcc
    22561 ttggggcggc gctgaacctg cttttgggtc ggcggtttgg caacaccttc gtcagcctgg
    22621 tcgcgacgtc ggcggtggcc ggcgctgcgc tcgtggcaac caaagccgtg cttttggtcg
    22681 cgtcggagct gccagcacgc ggcgcactgg ttgaccagct ggtgtcgggc gactggattg
    22741 tggcgggcga cttcaagttg gccgccggac ttcagctcga ccgcttgtct tcgatcatgg
    22801 tgatggtggt cacctggatc gcgctgctca ttcacatcta tgcgacgggc tacatggaac
    22861 acgagcccga ctacgcgcgc ttctttggct atctgaacct gtttacgggc gcgatgctga
    22921 tcctggtgct cggcgattcg ctaccggtga cgttcatcgg ctgggaaggc gtcggtctgg
    22981 cgagctacct gctcatcggc ttctggtatc aggaagacaa gaatgcgacg gcgggacgca
    23041 aggcgttcat cgtcaaccgc gtcggcgact ttggcttctt gctgggcatc tgccttctgt
    23101 tcacggtggt gggaaccgtc aagtacgccg actttggcag cgtcgcgggc cgtctgatga
    23161 cgccgtggtg gatgggctgg ccggtcgggt actggattgc aatccttctg tttgtcggtt
    23221 gtgcgggcaa gtcggcgcag attccgctgt acgtgtggct tcctgacgca atggcaggtc
    23281 cgacgccggt ttcggcgctg atccacgcgg cgacgatggt gactgccggt gtttacgtcg
    23341 tggcgcgctg ccacaccatt ttcgatacct tccccgaagc ggcgaagatg acggtggctg
    23401 gcgtgggcgc gctgacggcg cttatggcgg cgacgattgg catcgtccag cgcgacttca
    23461 aaaaagtgct cgcgtattcg acggtgagcc agcttggctt tatgttcgtc ggcgtcggca
    23521 cgggcgctta caccgcgggc gtgtttcacc tgatgacgca tgccttcttc aaggcgggcc
    23581 tgttcctggg cgccggttcg gtcatgcacg cgatgggcgg cgagggcgac atcgtgaaga
    23641 tgggcggact tggcaagcac atgccgatta cccgctggac cttctttgtc tactgcttgg
    23701 cgattgccgg catcgtgccg tttgcgggct tcttttcgaa ggacgcgatt ctggctggcg
    23761 cgtttgcagc cgaattccgg atggctggca cgatggtgcc gtggcaggcc aagctcgtcg
    23821 cgctgtatcc aaagctgctg tgggcgatgc tggcggtggc ggcgatgtgc acggcgtttt
    23881 acatgtggcg gctgtacttc atggtgttca cgggcaagtt ccgcggcacc gaggagcaag
    23941 agcatcacct gcacgaatcg ccgctgtcga tgacgctgcc tttgaccgtg ctggcgattg
    24001 gttcggtgct ggcgggtctg attggtgtgc cggcggtgat gacgcacggc ctgcccgaat
    24061 cggcagagca cgtcatgacg ttctttacgc agtggctcag cccggcgatg gttcccgcgt
    24121 cgcccgaacc gcatgagcac tgggtcgagt gggcgctgat gggtggcgcg ctggcgattt
    24181 ccggcgtcgg catcggcctg gcagcgatgc tgtaccgcgg cgggatttcc cagacggcgg
    24241 aaaacctcaa gcagtcgctc gggtttttgc acaacctttt gtggaacaag tattacgtcg
    24301 acgagatata cgatgtgctg ctggttcgtc cgctgcgacg gatcgccggc cttctgtgga
    24361 agggcgtcga caacatcgtg atagatggcg gcctgaccaa gatcggcccg ttcttcgtcg
    24421 acatctttgg tgcggtggcg cagcgtttcc agaacggcga cgtgcagcgc tacatcatcg
    24481 cggtgatggt cggtgctgcc ggcatcctgt ttggttcgac gtactggctt gcgtatcagg
    24541 gcatccaggg ccaggtcaaa gtcgacaagc gccaggtcac gctgaagctt ggcgacgaaa
    24601 ggatgcggcc tggtcagctc atttacgcca tcgactggga cggcgatggg aagtttgaag
    24661 acaccaatgt ccgcttcaac agcgaaatca agcacgagta cggaagcgcc ggtcagtaca
    24721 agatcgtcgt cgaagctcgt gaccccgtgt ggaacatggt caagcgctct gacgactggt
    24781 tcttcggcgc aagcgttccg cttgtcgcca aggttgagta ggagaacatc atgggcatga
    24841 tcctacccat cgtgacgttc ctgccgctcg tcggcgcgct tctgtgtctg gcgctcccgc
    24901 gggaagagca gcagcttcat cgcagcgtcg gttttggtac ggcgcttgta acgttctttg
    24961 cgtcgctttt tcttctgtcc ggtcttgagc ccgccaagat gaacttcgag gtggactggg
    25021 cttgggtgga atcgctcggc attcgctttc acctggggat cgacggaatt tcgatctggc
    25081 tggtgatgct gacgacgttc cttatgccga ttgtgtttct gtcaacgtgg tcggcgattg
    25141 gcgacaaagt tcgcgaattt gtcgtcgcca cgctgattct ggaaaccggc atgctcggcg
    25201 cgtttttggc gctcgacctg ttcgtgttct acgtgttctg ggaagtgatg ctgatcccga
    25261 tgtacttcat catcggcatc tggggtggag accagcggct caaggcggcg atcaagtttg
    25321 tcatctacac gatggtcggc tcgctgctga tgctggtcgc catcctgtac gtcgtggtga
    25381 aagcgcagaa agtgaccgga cagtggtcgt tttcgctgga gacggcgcag aagctggtgc
    25441 tgaccaagcc ggaacagatt tacctgttct tggcgtttgc gctggcgttc tgcatcaagg
    25501 tgccgctgtt cccgctgcac acctggttgc cactggcgca cgtcgaggca ccaactgcag
    25561 gttcggtgat tctggcatcg gtgctgctga agtttggtcc gtatggcctg atccgtttcg
    25621 cgattccggt cttcccgcac gccgtcgagg tggtggcacc gtggctcgcg atcctggcgg
    25681 tgattggtgt ggtctacggg tcgtgcgtcg cgtatgcaca agatgacgta aagaagctca
    25741 tcgcgtactc ctcggtggcg cacatgggct ttgtggtgct gggtctggcg atgctaaaca
    25801 accgctcggt gaacggcgcg ctgtaccagt gtttggcgca cggtatatcg acgggcggtc
    25861 tgtttctttg cgtcggtgtg atttacgaac gtcgtcacac ccgcaagatg gcggagtttg
    25921 gcggtctgtg gaagcggatg ccggtgttcg cagcgacgct cttgattttc acgatggctt
    25981 cgattggcct gccgggtctg tcgggcttcg tcggcgaatt cctggtcatc ctgggcacgt
    26041 tcagcgccaa agaacgcgcg tttttaggca tgccgcagat gttcgaaaat gcgcggcttc
    26101 tgtcggcgct ggcagcgacg ggcgtggtgc tgggcgcggt ctacatgctg tacctgtacc
    26161 agcgggtgat gctggggccg ctgacgaatc cgcagaaccg caagctgcaa gacatgtcag
    26221 cgcgagagac gtttgtgatg ctgccgatgc tggcgatggc gttctggctg ggtatctgcc
    26281 cgaacacctt cctaaagcac attgacccgg cggtcacgca gacgctgacg gtgttcaaag
    26341 acaagtacgc tgcgggggct gccgagagcg atgcccgcat gctcggagag gctgccaaag
    26401 ccaaggttgc ggagccggcc cccggaggaa atccatgacc ttcgacgcaa atgatctcgt
    26461 actgagcctg ccgctgctgt gtttgacgct ggcgggcgtg atcctgctgc tcctcgatgc
    26521 gttttcgcgg gtggtgctgg tcggtcgtga acaccacctg aagatgccgg cagagacgag
    26581 cgaagcggtc gctgtgccgc cgccgggttc gcgcaactac ctgatgccgg tgacggtgct
    26641 gtttctgctg gcgacgctgg caatcatcgt gtggcaggca gggacgctgc ccgaaacgcc
    26701 ggtcttgatt taccgcggca tgctgcagat cgatcgcttc ggcctgtttg taagcggagt
    26761 ctgtgtgctg gcggcgacgc tgggcgtgat ttcggcgccg tcgtttttgc gtgcgcaccg
    26821 gatggaattc ggtgagttct acgcgctggt ggtgttctcg ctcgcgggaa tggtgatctt
    26881 gattcaggcc gccgacctgg tgagcgtgtt tttgggcatc gaaacgatgt cgcttggcgc
    26941 ttacgtgctg acgggttcgt ggcgtcggtc gccgcgttcg tcggaagccg cgatgaagta
    27001 cttcctggtc ggcgcgtttg tgtcgggtct tctcatctac ggcattgcgc tcatctatgg
    27061 gatgacggaa acgacttcgc tggtcgacat caagcgggcg gcgatgtcgc gtggcgcggc
    27121 accgctgttc tacgtcggct ggatgctgct gttggtcagc tttgccttca agcttggcgt
    27181 ggtgccgttc cacatgtggg cacctgatac ctacgaagga gcgccgacgc cggtcacggg
    27241 ctttatgatg tcgtgcatca aagcggcggg ctttgccgga ctgattcggc ttttgcaagg
    27301 cacgctgccg atgctggcgc gcgacccgtt taccggctgg acgttcgtgc tgtcgtggct
    27361 ggcggtgctg acgatgtcgc tcggaaactt ggcggcgctc aagcaggaca acgtcaagcg
    27421 catgctggcg tactcgtcga tttcgcacgc gggttacttg ctggtcggtg tggtgtcgct
    27481 cggtacggtc ggcgagccgg cgcgtgggcc gcttctgttc tacctggctt cgtatgcgct
    27541 gacggtggtt gcgtcgatgg gcgtggtcgc ttgggtcggt gccgatgagc gcggcgagga
    27601 gcgggtacgt cttgcggact gggcaggtct tgccaagcgc gagccgatgg ctgcgctgct
    27661 gatgacggtt tctctgctct cgctgggcgg ctttccgccg acggctggct tcttcggcaa
    27721 gttctacctg ttccaagcgg cgctcggtga aagcaagctg ctgtggttga ttctcgtcgc
    27781 catcgccaac agcctggtgt cggtttacta ctatctgcgg gtcatcacgg cgatgtattt
    27841 ccgtgatccg ccgccgcacc cagcgacgac gccgattcag tcgacggcga tgcgcatcgg
    27901 cgtgctggtg acgacgctgc tcgtgctggg catcggcatg ttccccggct ttttgtcggc
    27961 gctctgcgcg caagccaagt tctagtccgc gccggcttcc ggcttcgctt cacttcccca
    28021 aacagcttcg ctttccatgc gcacgcaagc actccgcttt gcggaccgtc tcgcgatatc
    28081 ctgtggccaa ggagtcgcga tgaaagtcat tggtgcggga cttggccgaa cggggacgat
    28141 gtcgcttcag gcagcgctgc agcagctcgg atttgacccc tgctatcaca tgcgtgagct
    28201 gtttgatcac ccagagcatg tcgacggttg gatgtcggca gcgcggggtg agtatgtcga
    28261 ctggcagtgg tttttgcgcg actaccaggc gacggtcgat tttcccggct gctcgtttta
    28321 caagcagatg atggtggcgt ttcccgacgc caaagtcctg ctcagcgtgc gcgatccgga
    28381 tcgctggtat gactcgtgcc tggaaacgat tttcgcgatc agccacaagc tgccgatgcg
    28441 ctggatcggg ccgtacatgc cgaaagtggg accgatcctg aagatggcca gcaaggtcgc
    28501 ctgggaacag atgttcgaag gccgcttcct ggaccgcgag tttgcgattg cgcgctacaa
    28561 gcagcacaac gaagaagtca tccggcacgt acccgccgac aagctgctgg tcttcgacgt
    28621 caagcaaggc tgggagccgc tgtgcaagtt tttgggcgtg ccagttcccg aaggcgtgcc
    28681 gttcccgcat ctgaacgatg ccgacgagtt caagcggcgg attcgcaaga tgcaggtgat
    28741 gcagaccgcg atgctgggtg gtgtcggggc gctgttgatt ctggttttgc ggcgactttt
    28801 gcgtcgctag gcagcggtgg tgcctttttg ccgcgacaga taaagcccca ttgccccggc
    28861 gagcagcgac gccaagaggt agggcagaaa ctgcgcggaa tcaaacagtg tcccagcgac
    28921 gataggtcca atcgcgcggg acagtgaacc aagcgactgg ctaagcccga gtgtcccgcc
    28981 ttgttcggtg gcagcgacgg ctcgcgacag aagagtcgac agcgttggcg ccaacagtcc
    29041 ctgaccgaca gcgatgatga gcagtgcgac gattgccagc tcgcgactgg gcatccatgg
    29101 gatgatgcca aaggcgatcg tcagaatcag aaagccgaca cagccgatgg cgacttcgcc
    29161 aaatcgacgg gcgagcggcc caatcagacc gccttgcacc accgcaatca tcaccccgac
    29221 gaccgcgaac agccggccca tggtctgttc ggaaaaaccg aagcgcagct tggccagaag
    29281 cgcaaacgtc gattccatcg acacaaacgc cagcgtcgat cccgtggtga tgatgagcag
    29341 cggaccgacg acgggccggg acagcgcctc gcgcagcgtc ttcaccgaca aagccgtccg
    29401 ctgcgactga ccgcgtgcag aaggtggcag cgattctttg agccgaaaca gcgcgagcag
    29461 aaagtttgcc gccgacaggc ccgccgcgac aaacgccgcg gtgccaaagc caaagcgcga
    29521 cagctcggcc ccgacgaacg gcccgaagac aaagcccatg ccaatcgacg cgccgataag
    29581 gcccatgtat ttggtgcgct cggcgggctt ggtcacgtcg gcaacgtagg cctgagcagt
    29641 cgcgatgctg ccgccaaaca gaccggcaaa gccgcgggcc gccatcagcg tatacagcgt
    29701 gtcggcaagt cccgtcgcga aaagcgatac cgtcgatcca agcagacaga ccagcagcac
    29761 ggggcgacga ccgatgcgat cggacaggcg acccagaaag ggcgcaccga aaaactgcgc
    29821 gatcgaaaaa cacgcaaaca aaagcccgat ctgcttgccg tgggcgccaa agtgcatcgc
    29881 gtaaaacggc agaagcggca ggatcagccc aaagccgagc aagtcgagaa agaccgtcat
    29941 gtagacgacc agcagcgacg caaaggttcg ctgtggggga gccgtgtcgg gcgaaagcga
    30001 agtcatcgaa aatccttgcc tgcgtctggt tgcgtgtcgg ctggcgcggt gatgtggccg
    30061 cctacgctac gcgaaaagcc caccgccggg taggcacctg cttatacact gtggggtgat
    30121 gcgcagcgtg gttgtcgcgc tctttgtggg gatgctgggg ctttggggct gcgagcgaca
    30181 gacggggctg ggcagtgggc cgcgacatcc tgatggttcg gctgggcagg gggccgtagg
    30241 ctttgccgac ttgggcgggg ccgcgccgga tctgcggtct gtggcggtcg atttggccgc
    30301 tgtggcgtct ggaagccgca ccgatttggg tgtggctgcg cccgatgctg gggctgcggt
    30361 cacttcgggg ggcgtcgcgc gcttggcgga ccactcggat tcggcgacag acacgccggc
    30421 cccggtgctc aaacagtcgg cggtcagcgc cgatttgctg gtcttggagc tgggcgaagg
    30481 cgatgcggtg aaaaagctgt cggcgattgc ttcggcacag gccgaagttc gctgcgaaga
    30541 agtcagcgat cacgaagcgg gcagtgcgca cacccgctgt gacagccgct tggtgatcaa
    30601 aagcgcgccc gattcggatt cggtgctggc gacgtttgca gacgatgtgc cgggtgtgct
    30661 ctgcaagggg gaagaaacgg cgagctgctt tgaatcggcg gatgtcgcga tttcgtggct
    30721 ggcgctgccg gggctgccgg gcgggctcgt gatggtgagc ggatcgacgg cagatgacca
    30781 cgtctgggaa cgcgaaaacg caacgccggt ggccgtgtat ggtttggtgc ggcggggaac
    30841 gctggagccg ctcgagctgc gcgagctgct gcgtttttcg tctttgaccc accgcgaaaa
    30901 catccgcgaa gaccacccag agatgacccg tcgcgagcgg gaacgcgatc cgatgcgtac
    30961 cgacgaagac gagcgctcgg acctgtccgt catgggcaag agcaagcggc ggctgccgga
    31021 tcttttggcg accgtgaccg ccgaaaccgt ccgcaagttc gtgagcaaac cggacaagcg
    31081 ccaaatcgaa aagcacaaga tccggtacgt gtttgacggc attcgctatg tgccagacga
    31141 catctcgctc cagaccgcgg cgcactgtgg atcgcttacg acccactgcg gcccgctgca
    31201 ggactgctgc ggcggcaagt gcaaaacact gtcgaccgtc gagcactgtg ggtcatgcgg
    31261 gcgcgtctgc cccggcggtc tccttttgtg gagcgacgcg gcgtgtatcg atccggccaa
    31321 gggggtctgt ttctttggct gccaaggctc gcactacgat gtcgacggca aagagacaaa
    31381 tggctgtgag ctacacgaca ccacgccgga cctgaccgac ctggggacgc agagctgcta
    31441 tgacaagccg cttttgtggc aaggcatgct gctgtccgat tcgcgtgagc atcgcagtcc
    31501 ggcgctgtcc gatcgggatg cgaagactgg cgcggttgcc aagcggggcc aggttcgcgg
    31561 cagcggcggt ctgttctgtc aaaacgacct ggcactgacg gtgacactgc gcgacgggcc
    31621 caaagagccg tgctatcagc ttgcggtcga gggactgaaa cagccgctga ccatcgcgct
    31681 ttccggcatc gatgatcgga acttggtgct gccgcgtggc gcctatgtca gtggcagctc
    31741 gctgcggttc cgtctgacca agacgtgctt tgcgccggca gcggtcaagt ttgcggtcgc
    31801 gtttcacctg tgaagtcggg tgctacttgg tcggctgagc gacggggcga ggtacgattt
    31861 cttttacgat cgccgtcgtg cccgcggatt ccagcttggc gccgatgtgc agcgggcaga
    31921 gcgtgcagcg ccgcagcttg gtcccatcga gcattgcgtc cgcggaccac atgccaacgt
    31981 gaatgcgcgg cgtcccatcg gggtgccaca gaagcagcag atcgaccagc agatcatcgt
    32041 gggtcggcca ctcgatttcg ctctgtggcg tggtgccaag ccgtaccgct tcgtctttcc
    32101 agaacacgtc gcgcggctcg tcttcaccgt cggaaatcgt caaggtgacg cggcgctcgc
    32161 tgaccgtgct ttgggtgggc ggcagcgtct tgcgatcgat gtggatcagc acttcgccat
    32221 aggaaacacg gtcgccgtgc tgaatcgccg tcgctgtcgt cactttcgca aagttcagat
    32281 agagcgtctg cgcggcccca ccgggcaaca gccaaagccg tcctttttgt cgttcgatcg
    32341 aaaaacactg cgacggcagg ctcgggtcgg caagctgcac atcggcggtt ttggcggacc
    32401 cgaacgaaag ctgaccgcgc gtcgtaaagt gccagcgcgg tggttcatcg ccatcgagaa
    32461 tcagcagacc gagcggaggt ctgggcggct cggcagggct ctcgatttcg cgcacatcaa
    32521 tttgcgtgcc cgccatcgtc agggtgtcgc cttcgcaaag cgcagtcaca ccaacgatgg
    32581 gctcgccgtt gcgctgcacc acggctgaat acttgtgcaa aagcaggtgc ggcatgcccg
    32641 cggcggacca aaaaatcacc gcttcgacgg ggtcttgggc atagaggcgg ctgggtttgg
    32701 gttcgccgat gtagatgtcg ctgtgtgcgc cagggcagat cctcagcagc gcttgctcac
    32761 actcgaccag tcgaggggcg gcggcgggct cggtgacttt cagcgtcagc cggcgcggtc
    32821 cgtcgctggg atacgacaga tcgggcggga cgaagaacac cttgatgatt tggctgccga
    32881 cttcgatgac gtcgcccgac gacagtggca gcggacggtt cactttgacg gagtttagga
    32941 cggtgtcacc actgccgcgg agcggaaaca gaaacagcga tccggcgtgc cattccagcg
    33001 cgcactgccg tctcccgagc agcggatcgg cgggcagact cagctcgcag tcatcgccgc
    33061 gtccgatccg gatgctcggc agatcgaagc gaaggcggcg caccgagtca gaaccttggc
    33121 ttacgaggtc gaggtcgatg cgcatgatgg ctagacagcg attctacacc gacttttggc
    33181 gggcacgacg acggagcgtg gcaaatccga gcagcacaag cgatgatccg agcgtggctg
    33241 cgcccgaccc acgcgactga cccacgtcac aagagcagcc gctggcatcg accattgtgg
    33301 tgtcgacgat cgggtcgcca ccatcgggat tgccaccact gccaccgccg tcggggttgc
    33361 cggtcgagtt gggcatgcac tggctgctgg cgttgcaggt cgagttggcc gcgcaggtgc
    33421 cgcagacgct gccgcagccg tcgtcgccgc actgttttcc gccgcagctt ggggtgcagc
    33481 cgcaggtcgg cgggggactt gctgaaaagt ccgaaccgtc ggtggcgcag cttgtcccaa
    33541 tgtaatacga gccagtttgc gggaacgtca ccgtcgttcc cgtggagtct ttgaactcga
    33601 agcgatagcg ctggcaggtc gaaggaaggc cggtcagcgt cgtcgtatag gcgccgtttg
    33661 tcgctgtgcc gaactttcgc tgcatcgtcg agcacttgcc ttcgacgttg acgatcgctt
    33721 gacttggtgc cgcgctgtct ttccagttgg cccacatgtc gatgctggcg ccggtctgcg
    33781 ggtagtgcga tcccgacgga attttgtaat ttcccgccgc tgcaccgcca aaatcgccga
    33841 ccgaatagcc ggcatcggtg acacccacgc cgaccgacgg tcccgccatc aggatgttgt
    33901 agcggtgccc gtttgatccg tcgctggcgt agccgcactt gttggtcgag gcggtttcgt
    33961 aaagccacag atagaacgct tgcttgggat cggtcggcgt cgcgatgatt tcgccgctga
    34021 agctggtgtt aaaaagcggc gcgcgaccgc tccaggccgt gcacgtcgag ctacaggctt
    34081 tggtgccgcc ttggcaggcg cagctcgccg cgcctttgca ggtgcccgga taaatcgacg
    34141 agatgttgct gaccaccgtg cacgccgagt cgtgtgcaaa gtagttttgc tgcttcatct
    34201 cgtcggagtg aaagcgcgcc gcccgaccca agttcagatc gtagtagagc gggtttgtcg
    34261 gcatgtaaca ggatttttca agacacgccg tcgctggaca ggccgccaga tccgatgccg
    34321 gatcgacgcg ggatcggttc ataaactcgt gaatggtgcg ttcgcgccag ttgggaaagc
    34381 cgccagtcga ctcgcccact gccgatgccg acgaagaaag cgcaaggata gaaaggccaa
    34441 ggcaaacgga gatcgttttt ttcatcaggt ccccccctcg gtgttggccg gaattttcta
    34501 ttttaccttg cggcttggat gctccagcga gaagaactcg cggctgacgt cggcatggag
    34561 ccgcccgagg caatcgaccg ggcatttggc acagacctcg gaggcaaagc tggcgagccg
    34621 ggtgaggacc gccgaagaaa gcagtccggc gagagatcgc gacacatgca tggtctgctc
    34681 gacgagacgc gacgactggg tgagcaggcg cgacaggctg ggcccgcgct ggcgaatcag
    34741 ctgatcgcgg aaggcgaccg ccagttcaaa cgccgtctgc tgctgaagct cgaaacgcgg
    34801 ccactcttcc gacggtgcac acgacagtgt cgtcacgatg gtttgcagcg cttcgtcggc
    34861 gtaatcgagc acaagcaggt cttgcgccga cggtttttcg atcccggcga aaaaacgact
    34921 cttgagctgc tgtcgccacg acagtcgatc ggcgccaaca agcggcgatg aaccgggcgc
    34981 cgaaggccca atcgcaccat cggccatgtc ggtcagccag cgacgggtgt tttcggcgac
    35041 aagctgcacc acgcgactgt gtgcgaagtc tttttcgaca tcgtgggccg ccaaaagaca
    35101 cagcttggaa tcggccagct cgccagacac cgtgtagtag accgccatgt gacgcagcat
    35161 gctgccgagc cgttttttcg gcgcatcggt cttggcgagt gtcgtcgcca tctgctgcca
    35221 aaagggctgg gcgcgcagag gtgctgcgac ggcgggcgtc tttggtttgc gggtcgtgct
    35281 cttgggcggc gcagcgcgat ccagctcggt ctgggtaaat cgccagccgt cgccaaactc
    35341 gggtcgggaa aacagactgc gcagcgctgt cagcgtcagc cggcccgtcg gctcggggag
    35401 cttcagcgga tcggacacaa agcgtgacaa aaagcgcagc gccgtcatcg cttccgccgg
    35461 caccgaccgt ccattctgga gcgttttttc cgccgcatgc tcgacgagtg gcagcagccg
    35521 tccgatcggg gcggcgacaa accgggtgcc actttcctgc gcgacttcct cgcacagccg
    35581 cgcggctcgc tcgggcgact gcccggtcag cacataaccc gatcgcagct cgccgctgac
    35641 atgcagacac agcaccgcca gcgtcgtcga tgcatcggga ttgtcccgtc gcgccacaat
    35701 cacaaaacag ccaagcgtat cgcagcccga aagatagccc tgagcatcgg ctggactggg
    35761 cttttgtccg tcgagaatcc gcgtccgcag ccgcagaagt gcaccttgca aaagtcgctg
    35821 ctgcgtccgg tcggtcagct cggcgcgaat cgaatcgagc agctgaactg catcttcatc
    35881 gccttgcttc tgcagcgcct cgacgatttt cggaaacagc atcgacaatg acgagctgcg
    35941 cagaagtgct tcatacgcga cgctggcggc aattcccgat ccgacgcgcc agcgatccag
    36001 ctcttcgaac agcatgacca gcatgtccga cggaatcgcg ctcatcatcg acgccagacc
    36061 ttcgccttcg gcgggatcgg attcgacgcg aaccagtgca tccgacagcg gctgggcgat
    36121 gatttgcagc atttcgtcgg gagacagcac ttcttgcagc ttgccggcga agcttgggtc
    36181 ttccgacagc aggatgccca tcgcgcacga tcgggtccgc aggtcgagct tgtcgtcgga
    36241 caccacgcgc aaaagcgagt ccgcttcgct gccgagcccg agcacatgaa acagatcgcc
    36301 gtacagctct acgtccgtcg attcgacttt gccgctgcgc atccggtcga gcagatactt
    36361 gcgggcatca ccgacgctgg ccacaccaag ccgctcttga agatcggcgt ggcttaccgg
    36421 cggatggaca aacaggtctg cgacaatctg ctgggttttc gccgcactcg cccacggcgt
    36481 ctttctgtcc ctacgaggca ttttccggcc ccttatagca cggcgaggga agcgcggtga
    36541 ttgacctggg gccgtcggct ttgaaacact gacttgggat tttgaccatg aaaaccgtca
    36601 agctgtctgt gcttctgtcc gcggttttgt cgtcggtggc gtgccaaaat gggcctgcgc
    36661 ctgcgggatc ttctttggcc ggtccggaaa cgattggcaa aaccgtggcg aaactgaccg
    36721 ctgagtcggc tgcgctgccg ccgcttctgc gttctcgctg ggcggtcgaa tacgtcggac
    36781 aggtgtcgca gcttctgccc atttcaccgc ggcggctgta tttttctgcc gatcggcgcg
    36841 tgtatttgtc cgacgccgag gcgcgactgc aaaacaatcc gcccccaccg cggacgttcg
    36901 aaatggtgga tgagtctgcg tattacaccg ggaatcttgg ctcgccgttg tttcatgcgc
    36961 ggcttatcga cttggttgcg gaagggcagg cggccaagct ggcgggtcgg cgcagcctcg
    37021 atttgtatgc cgcggcgatc gggccgagca agctgcttgc ggcggcggga ctggatgcgg
    37081 tggcggtgtc ggcgtcgccc aaacagcggg cgctttattc gcaggccggc gaccaaggcc
    37141 cggtgcgtct tgccggtcgt gacgtgagcg gtcgggtgac gtttctgtcg ggtgattttc
    37201 cagccaatcc gtcgctgcgt gcccaggtcg gcaccggcta cgagtttgtg ctggcgaaaa
    37261 acgtgctcaa gcgcggctac atccgaccgt cgggagaggt cgcgcccgac agcctgataa
    37321 accttggcgt tcccgacgct gattacctgc gttatttgaa ggaaacgcta aagcctggcg
    37381 gacggctgct ggtctacaac ctgtgtccaa gccccaatcc cgcgctctac gatcccgacg
    37441 cagactgtcg cagcccgtgg agccggtccg aatggcaagc cgccggtttt gtcgtccgtg
    37501 acttcgagcg caatgacacc gaagtcgctc gtcagtttgc cgtcgcgctt ggctatgaca
    37561 aaccgccgct gtcgctgcgc atcgaagacc tgtatgcgat ctacacgctg ctcgaacgtc
    37621 ccgcgagctg agccacgcta tttgatgctg taaaaatcga gcgacggcag cgcttttttg
    37681 tccgcaccga cgccacccac aagcagcgtc agatcgtttt cgagggggat cgccagatgt
    37741 tcggcgcggg catttggcat tcctgctggg gctcggctct gctgcccggt gtcagacgac
    37801 agaaactcgc agttggacag cacgtcgccg gcttggttat agccgccgca gatcaccagc
    37861 tcgccggcgc tttcgtgcag gctcgcgccc cagcgtggcg tttttagtgc gccatcttgt
    37921 tgcgtcgcag tgcgggcggc gatgtcgacg gtcagcgtcg atccatgaac ggttttttgg
    37981 ccgctgccat cttcgctgta gccgccgatg atgagcgcca gaccgttttt tagcttggcc
    38041 gcgaggtgcc cgcgacggga cacaggctcg gtgccggcaa aagtcagcgc ttggaagatg
    38101 tttcgttcgg gagcataggt ctcagcgacg ggttttttgg ccgcgacatc gcttgtcgaa
    38161 agaccgccaa acagcagcgt tccggacgac agcaccgtca tggagtggcg ataacgcggg
    38221 accacaagag ccgtcgtgcg atcgacgggc ggtggcacgg tggcaacgcc gacgcgcgcg
    38281 agcatatcgg cactttgcgc cggaaagcgc ttgaccagta ccgcctgacc gctgggctgt
    38341 tcggtgttgg gatcgatcgc tccacctgcg acgaggaata cgctgtcacc aatttccacc
    38401 gcggcggggc gatacatgcc gggcttgccg atgtcgccga tttcgacgga gggcggagac
    38461 cagaccgccg acagcgttgt caccgagggg tcaaacagct cggtctgggt gatcatgtcg
    38521 gtgccggtta cagtccctcc gacgagaagt ccttgctgac cgatcgtggc gtcggcgctc
    38581 ggcacaagca ctgcaccgcg gcggcctttg gaaatggttc cgccgtcgag aatctggaac
    38641 gttgagtgct taaacaccca ggctttcgtc gacaacagcg cgccttcgct ttcgccgccc
    38701 accagcaggg cacccaggcc cggttcaggc ggactggtgc ggcgaccgcg cagcgcacag
    38761 ccagccagcc ccttgcggcc ttgcgcattt tgcatgctgt cgtcgggcag tttgagatcg
    38821 ctgctggtga caagccctgg cttgccgacg tagaccgcga cttcgctgtc ggcaagctgg
    38881 attccaaacg tcggcgatcg gcctcgcccg acgaccattc ccgatccatc aagcgcttcg
    38941 accagcagtt gggtctgctg tccatccgtc ggggattcga tggttagcgt cgtcgaaaaa
    39001 tggcctccgg caacggctgc agtcgtctgc tgatcaccga gcgtcatccg cacctgactg
    39061 gcgagcaaaa acgggtcgtc gctccccggc ggggtgatga tcttgacggt gaggctgtgg
    39121 tttttgctgc acgacagaag cagcagcgac gctgccagaa gttgaaacct acgctgcgag
    39181 ctaaacatct cgattccttg ggcgaaaact agctgccgct accgacgagc gcctgcgctt
    39241 ttgcggagag tagcgcttgg tcgccgcaaa cagccagggt tttttgccga cccttgtgcc
    39301 cgaagatgac ctcgacgcgg cttttgggga cggaaaacgc tttggcgatc gcgatgcaga
    39361 ccgcatcatt ggccgcgccg tcgacgggcg gagcgctaac tgccactttg agtcgatcgc
    39421 catggatgcc gcccacgctg tcccgagagg cgcgcggcat cacccaaatg tcgatggtga
    39481 ttccgtctgc ggtttggcga aacggggcag ccaaatcacg actccttggc ggtttggcgc
    39541 cgcttttcgg cattttggat cgcaatgtcg cccagctctt tgctgcgccg cgtggcggct
    39601 tcgacggcat tgatcagcgt ggtgcgcagg ccgccggctt ccagcgtgtg cagtccggcg
    39661 attgcggtgc cgcccggcga cgtcaccatg tctttgaggt ggccgggatg ttggccggtt
    39721 tccagaagca gcttggccga cccgagcacg gtgtgggccg ccagcgcgac ggcgttgtag
    39781 cgcgacagac cgactttgac gcccgcgtcg gacagcgctt cgatgaccag gaacatgtag
    39841 gccgggcctg aacccgaaag acccgtcact gcatcgagct gatcttcatc gagcacgacg
    39901 gtaaagccga tcgcgccgaa gatttgttgc gacagcttga tgtcgtcttc tgtcgcggtg
    39961 ttgccagccg aaatcgctgt ggctcctgct ccgacgagcg ctggcgtgtt cggcatgacc
    40021 cgaatcaccc gcgtgctttt cggtagatgc gactgaagca cttgcagtgg cacgcccgca
    40081 gcgacggaaa tacacagaca gtgcggcttt agatagggtc cgacttcatc catcacgcca
    40141 agcagaacct gcggtttgac cgacagcaca atcaatccag cgcggcgcac cacgtcgacg
    40201 ttgtgcgtcg tcatgttgat gccgtactgc tgccgaagct gctggcagcg gctctgcctt
    40261 gggtcagatc cccagatttg ttccggcttc accagacccg cggccaaaag ccctttgatg
    40321 agcgcctcgg ccatgttgcc agtgccgaga aagccgatgg tgccgggcag cgttttgtct
    40381 gggctcgatg cggtatccga cgagggagca gcagtggttg tcatggtccg gcatggtaac
    40441 gcggcgcggc gtggtcgcaa agtctgcgca gcctgggcgc ttgcgggccg agcgctgccg
    40501 cgggcacact accgacatca tggcgagcgg tgagaagcgg tacgcagcag cagatgttcg
    40561 cgaaattttg cgacgggccc agcacaaaga cagcctggct gtgcgcagcg attcggcggg
    40621 tctttccgaa gcggagcttt tggaaaacgc caaagagctg ggtttttcgc cgcaagacgt
    40681 gaaaacggcg atggttgcgt ttgatgaaga tcgccaagtc ggcgtcgccg cgagtgagct
    40741 tcggcagctt gcgtatcggc ggctgtcgac gcacgcaatc gtctatctgt cgtttctggc
    40801 catcctgaca gcgacgggag cgtgggcggc tgcggggact ccgctttttg ttgtcgcgat
    40861 cgtctgggcg atgctgttcc tgtttcagct gcgcggcgcg ctgtttcccg atccggacaa
    40921 gctgcgagaa gcggcgagac agcggctttt gtcgcgaaag ctcaaggaaa gcggcaagca
    40981 atttggcaca gcactggcga cgggcgcggc gaagcttttg acgctgtcgg cgcagaaaat
    41041 cgacgaaggg gtcaagcagc tcgacaaata gcgacggtgc aggccccaaa acggcaaacg
    41101 aatcctacag agaaaggtgc ggctgtgctg acggtggaag aaatccagat ggtgtttgag
    41161 caaggcttgc ccgcgagcaa agagtggggc ctgctcgtcg aaagcgcatc gccggaagga
    41221 aaagcgcggg ttcgctactg ctttcgtccc gacatgatcc gccccggtgg aacgctgtca
    41281 ggaccgacga tgatggcgct tgcggatgcg gcgatgtttg cgtcgattct ggggcgactc
    41341 gggcggctgg aaatggcggt gacgaccaac ctgaacatca attttttgcg tcgcccagcg
    41401 cctgccgatc tgatcgcgga gtcgtcgatt ttgaagctgg gcaagcggct ggcggtgctc
    41461 gaagtgtcgc tgttcagcgc caccgatgat cccgaatcga tcgagccagt cgcccacgtc
    41521 accggcacct attcgctgcc cccgaccctt gtgccgcgct aggccatccg attttcccat
    41581 ttacccgtcg acaaatcgcg ttttgtatcc gctttacact tctttacgtt cggcaaacgg
    41641 acgcttaaca gtcgcgccct aacctcagtg acagataggc gctgggcaca aagccgcaag
    41701 gttgcgaagc gatgcagccc agccaaccca ctgaacacgg gagaacgacg atgtgcggat
    41761 tcattttcgg aacgatttgt cttgttgcgc tggccaaagt gctccgtcgg ggctgcggtt
    41821 ttagacgggg ctgcggtggt ggtggctgcg gtggtggtgg ctgcggtggt ggtggtttcc
    41881 acggctacgg tcactggcag ggcttcggcc caggcggtcc gggcccaggc ggtgaaggac
    41941 cgggctttgg cggcggacga tttgccgact ttgtgctgcg gcgcgtatcg gcggaactgg
    42001 atgcgacgcc ggcgcaggaa aaagtgctgc gcgaagtgct cgacggactg cgtgattcga
    42061 tgggcaagaa ccgcgaagtg ttgcgggcgg cgcgcgccga tgtggccaag acgctgcgga
    42121 ccgaaaactt cagtgaggaa tcgctcgccg acgtgattgc gcgccacgac gaagtccacg
    42181 accggatgcg caagtcgctc gtcgatgcgc tggccaagct gcacaccacg ctcgacgccg
    42241 gacagcgcga aaaactggcg aacttgatcg caaacgggcc gtttttcggt cgcttctaac
    42301 caaaacgact tcgacgagga gcacaccatg cgacgcaaac ttctcatcgc cttgctgggt
    42361 cttggcgtga tcgctggtta cgggggcgaa atctatcgca tccgccagtt tcacaaaaac
    42421 ggcggcgcac cgtttggccg cttctgccag cagcgtggac actgctgcca cggtggacag
    42481 cgcgacagtg ccccagccgg ccctgtgacc ccggcggctg cgcaaaccga ccccgccgcc
    42541 acgaagtaga cacccgccgc ctttccgtga aaggctccta gaccatggct tctatccgcg
    42601 cgcttctgat tgacgacgac caccgaattg ccgagctgtt gcaagcattc cttgggcaaa
    42661 acggggtgtc ggtcgtgcac caaagcgacg gtcagcgtgg tctgcaatcc ctcgatgccg
    42721 acagtttcga tgtggtgctc ctcgacgtga tgatgccggg gattgatgga ctcgaagtct
    42781 gtcgtcgcat tcgcagccgt ggcagtcgca ttccaatcat catgctgacc gcgcgcggcg
    42841 acgaggccga tcgggttgtt ggactcgaac tcggcgccga tgactacctg gccaagccgt
    42901 ttagcccgcg tgagctgttg gcgcggattc gtgcgctttt gcgtcgaacc cagccaacct
    42961 ccgaaagtga tcggctggtg gtcgcggggc tgtcgcttga tctcgccgcc cgtcgcgtca
    43021 gcaaagacgg gcgcgaggtc gacatcaccg gcctggagtt cgatctgctg ctggcgcttg
    43081 gcaagcgggc cgggcgggtg gtgccgcggg atgcactttt gtcgctggcg ggtcgcgatg
    43141 atgtgaccgt gggcgagcgc accgtcgatg ttcacatctc gcatctgcgg gccaagctcg
    43201 gcgacgatcc gaagtcaccg cggcttatca agaccgtgcg tggcgtcggc tatgtgctga
    43261 caaaggagtc gtagaccatg cgcccccatg cgcgccattt gcaaaggcac tgcgccccga
    43321 ttacgcgacg ggaacggatg gggcactatc tgcggtcgcg gctgcatcgg cggctgtttc
    43381 tgtggctttc ggcggcgatt ttgacgacgg tgctgacggt catcggcgtg ctgcgtgtga
    43441 ccgggaattc gtttgattgg cgacggcatc agcaaggaat ggcccatttt gtcgtcgagc
    43501 gtgtgctgtc tgtttgggac cgcccgacgg agcggcggtc actttttcac gggatggcgc
    43561 acgatctggg ggtgccggcg cggcttttgt tgccgcatgg ggaagtgctc gatggacagg
    43621 actttccgcg gcgctgccgt gtgaactttg tcgcgcccct ttttcgcgat cgattactgg
    43681 tcggcaatgt cgagctctgt ttcgacggcg gtcttgggcg ttcaccgctt ttgcgacctg
    43741 tgctggcact ttttgcggcg cttttggtgt tgtggacggt ggcggggctc gtcgcgcggc
    43801 ggattggtca gccgctcagt gaactggccg acgtggcgcg acagctcggc gaaggacggc
    43861 tcgataggcg ggccaagctg cggcacggcc aagtcggtga agtcggcgag cttacgctgg
    43921 cgataaacga aatggcgggt cgaattgaaa aacagctcaa agaccagcgc gagctgttgg
    43981 cggcggtgtc gcacgag
//
LOCUS       DAORGN010000015        41731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_319_length_41731_cov_6.054132, whole genome shotgun sequence.
ACCESSION   DAORGN010000015 DAORGN010000000
VERSION     DAORGN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 41731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41731
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_41731_cov_6.054132"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKL17_03235"
     CDS             complement(<1..399)
                     /locus_tag="PKL17_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNK43768.1"
                     /translation="MSKRLYVGNLSYSTTEPELREVFERIGAVTDVKVMMDRETGRPR
                     GFAFVEMSSDEEARDAISQLNGSELGGRTLKINEAQARESGGFRGGGGGGGGGGYRGG
                     GDRRGGGGGGGGGGDRRGGGGYRGGDRDFGG"
     gene            714..2027
                     /locus_tag="PKL17_03240"
     CDS             714..2027
                     /locus_tag="PKL17_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HNK43769.1"
                     /translation="MSSQCPALRRAAPSRRQAIGFGGSLPLLMASIGLLSCTQDPPIP
                     ADDGPPDLVSTAPDLAVPGDPPDLTMPPPSPDLAIVPLAPCSWRDRFAEAFGHAFAKN
                     DVAFVTTDGKVTRGMDQLDFAAQKSQIPDIVKVKGNANRISEIQLLDPATGKVLASNK
                     AAFVYGLGYAMWLEVNAYGEAGITEMTLIRQFAGELPTYQQLVGLYRVLTKTAEAQVP
                     AADRGDFQQAAAKLMSAIESGSLTFALGTGHAADFPATAQTANGKTFSFQLSNGYGMD
                     PQIGFQRNGVWGPLWQTYSLTGAWSASTPGMQPKCGGCTTDSFGGTKYLFCEGPLPRK
                     NAAAVCASHGGYLATVDSVFVNSFLRGMAQNAGKAFLGLDDLAEYGKYVWATGKPATY
                     TNWGQGQPDHYMMQERCVQLYDGKAITQWNDIDCASPLPYLCQLP"
     gene            complement(2071..3732)
                     /locus_tag="PKL17_03245"
     CDS             complement(2071..3732)
                     /locus_tag="PKL17_03245"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HNK43770.1"
                     /translation="MARSSLGFVVLGLVGSCNPATGNVDEDGPMAPDMSIPPPPPPPS
                     CDEGRIRCNANRDGTERCMSNSWIGADKCNIAGGDACIDGKCLTPCEQVTRGNVGCSF
                     YPANLWSTSLVGDFGIVVTNTSDRLTASVTLSDASGVIETKSIPPAMGNPAAGVAIFR
                     LSHDKNKLAETEIAKKGFYLKSTAPVAVYQFHPIDAATVFSGSATLLLPEHVMAKNYF
                     VMSYTYNAEHMTFPPQGAGLLAVMALTDDTMVEVTPPVATAKGPGVPALRAGETVRRL
                     LKRLEVMEIVQANSREDISGAVVKASAPVVVYGGAGGVTIPASAVGGNHLGVQMFPLE
                     TWGKRYVGAKVKQRNLSDKDYYRIVASVDNTNVTIKSTTMLPAVPPLSKGQIYEFATD
                     ADFVVESDQPILVFQYMPAWGNLTGRYDPKEFPSGVSARCPFIGTADDVKCLGDANIT
                     PLVPVEQYRNDYIFYVPQTYSYDFINVTAAPDAKLSLDGQAITTPFRGIADGTIGRMI
                     IRIEKPGNHRITSTKPFGLLGYGYAYATSYSYAGGLNLDKINPIE"
     gene            4136..5254
                     /locus_tag="PKL17_03250"
     CDS             4136..5254
                     /locus_tag="PKL17_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43771.1"
                     /translation="MKFFRLILLALICSLSVVGRSTSSPNVRTAYLQESGGTSFGNPS
                     TMAAVPARDSSRFPIQLGSRSEAVLRRRLTLFVPPNSEIPPFEPSPADFEPAPALVDP
                     RPRFRGELDEEILARLCSLPIKSIKPLGGGSSIKLRVTFVDGSQAAVKPNQLRITRYQ
                     AEVAAFKLARTLGIMSVPPSCVRTFSKEQLQANMPKDLRERMDQELVVDDKGNVKTAV
                     IQWVPGLRNMKLEQADWWRPLLKAGEPIPNGKHKRVAEISTLLLFDYLLLNFDRWSGG
                     NTTEADGEMIYIDQGASFGPDKKHGHSRLMLKTLRWSERFPRTLAQTLFQLDLPALRS
                     DFGDLVSDDEWDSFLYRVEHARAYLKSLKKLNHKDSLF"
     gene            5314..6942
                     /locus_tag="PKL17_03255"
     CDS             5314..6942
                     /locus_tag="PKL17_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43772.1"
                     /translation="MLSRPRGRLVTLSALSVALFSVQLASAKRTAEKPVVAKRATAES
                     APAVSAPASVLPFVENDFALARAQALKSGKLLFVDAWALWCHTCLSMRNFVFTDVQLQ
                     PLANRLVYAAIDTEQPSSAAFLARFPISTWPTMLVIAPSEQGDQIVARFTGALSAKEL
                     SSRLGAVLNQQAQQQPKLAKADALAAAGQHQQAAAEYEEALEEPGSRPRALLGLIQSL
                     RSLGQHARCADLYDRSFLQVGKSALATDFAAYAASCMDKLDDVDRRQKLRRQLRTDLG
                     NLLDDPSADLAIDDRSDAYGTMVELSDALGESSEGDRLTTKRLALLEKAAATAASPVL
                     AATFDAHRFDSYRRLKRWAIAEEMLLASAKALPSDYNPPARLARLYYETGRIEKAEAQ
                     IGTALSHCLGPRRASMFELRAAIQNSQNRSREAMASLMEAVSVLTANQPAGAPESARV
                     LSLRQQLAGLEAKRKEQPDDSDGAPQSVPPAQQNAKPGRKNGGQKTGDRKNADKKNDG
                     ASERKSPPPRYEPGTPVAQRKRDRRLATRDANLK"
     gene            7086..7718
                     /locus_tag="PKL17_03260"
     CDS             7086..7718
                     /locus_tag="PKL17_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43773.1"
                     /translation="MSVSHLAKVSPSQLGFVRAFLWQLARTGQVSESAVEQQAAVDAA
                     RLIFSISRSRDELWRFFGEFRQLVESGNDSALSDALRAVLTPAAAQEPVPAEVPAPVV
                     EAVEKVEAPAPVVETSAKAEVPASVPPAAMSKSARRKQAKQAADAQVTPTAAPPPATD
                     SVPAQVVESASSDDAPMATAGDTPDDEGGDASNGPSAHVIHGSDAANETT"
     gene            7792..9930
                     /locus_tag="PKL17_03265"
     CDS             7792..9930
                     /locus_tag="PKL17_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNK43774.1"
                     /translation="MRAQPMLPPGIGVALAMLGALLASACSYRHRLLVAIQDPTQWAD
                     DPISRGELYRQLGSDAMLALCVAAMTWLVVLALLPLSRMRALRVLGLGLLSALLLLLG
                     MLLQAQHGTLLAMRSGLTLELLKESLDPAAIRESWSLLPRSELPYLLMPLLGFWLSWL
                     SLHLHRIVRIGFLLSIGVVTVALFTWAVRVPAAPLQTALLHHPLGFLATELVSAKRSQ
                     HALAQVGVGVAVANAATRNQAPTESGGPAGGVDGPPIGDDAADSPPVEEETQAVAPPS
                     QARPQLALTDSMFAYLPSERPIQKAKPQAVSGPLNVIVLILESTGADYALLPIQSGKL
                     AMPFLHSLTEKGLFLRNHFSAGNSSPRGIFSLLSGLYVMPEVGIWDVRKDIYLPSLTT
                     YLGSTYQHFLVTPASLDWYFPHNYLLHSGFTELWGYHNLPIRKNAPGGRAHARDEAES
                     VSVFLQKLSTAAAKSEPFVAVYYSFLSHWPYPDYGPDSHVVPPTKALHLYLNNLHYLD
                     KQIERIYERAKALGILDKTIVVIAGDHGEAFGQHPHNYTHSRQSFNENYRTPALLLHP
                     QLFPPRVVSEPTSHVDFLPTILDAMGRSYDPQLLQGESLFQDHFRRKYIFLYGNEDTS
                     SSISENQVKLQVSFRDGSCWVYDLRVDPTEQKRLSCAPHKAQYEALLEYRKRQRQALR
                     TYNAVFRRELQQGNSPSAMLVPDASPVGRN"
     gene            10013..12079
                     /locus_tag="PKL17_03270"
     CDS             10013..12079
                     /locus_tag="PKL17_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNK43775.1"
                     /translation="MSTRFRFPRKTRAFLFGLIPLLTACEKTPVPVDPGRVTLHRLNR
                     DEYDNTVRDLLGTSQRPAQQFPSDDSGYGFDNVAQVLSVSAPHVERYLRAAESLIDEA
                     LRPDGFPLNKLLQAGDVEVRYRRRNQIFFASNSSIPIRIEVPTSANYVISTSAFGEQV
                     PPDPAQMVIAVDGVLVKTLNVNGTESAPDQIQIEVPLTAGTHMLSFGFANDLYVPDPD
                     PGKTKDRNLGVYWFRIHGPVGVNPENAIRRRTITCTPSTSDQNEWQRCADQILSRFAR
                     RAFRRTVSTEEVTELRALSQEAWRAGDDFLTGLRPALIAVLLSPRFLYRVEIDQNPSS
                     LTPHRLNDFELASRLSYFLWSSMPDDELLRLAEQSVLDDDDSVLSAQVARMLDDPKAE
                     NLLHRFASQWLQLATLDGVTPDLKRFPTWNESLRSDMKQETLLFLRDFMPLLGSRNLP
                     ITRLLSEPMTYLSPTLAKHYGMDGPGPDVPWEQTTLLGTPRIGLLTQGSVLTMTSYPG
                     RTSPVKRGKWILEQLLCSPPPPPPPGVSTDLTAMMGNGTLRQKLEAHRQKPECAGCHR
                     LMDPLGFGLESFDAVGAFRNTDEGQPIDTTGQLPSGATFSDHTELTELVKSDPRYLRC
                     IAMKLFTYGIGRTPVDSDETQLANIIEQLGSGEGTLRQLIFAIAKSEPFRSRRGETDS
                     VEGVTP"
     gene            12076..13386
                     /locus_tag="PKL17_03275"
     CDS             12076..13386
                     /locus_tag="PKL17_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNK43776.1"
                     /translation="MTAVTYPSRRQFLRSGGSFIALPFLESLLPKTARADGKSPQRLL
                     FYFVPNGIHMPAWTPSSEGTDYVIPPILKPIEALRSELLVVSGLRNLPARPDGAGDHA
                     GGTSAFLTCVHANKSETDVRLGISVDQVAAQKLGTGTRFASLPIGMEGGASVGGCDSG
                     YSCAYSQNISWIGPKTPLAKIAGPQLLFDLLFQDSSQGTGVAEKRFRHRQSVLDFVRR
                     DAGSLRGKLGRSDRDKLDEYLHSVRDVEQRIQTMSGSCDTPMPPTDDVRLGEQLKAVS
                     DLMVLALRCDLTRVLTFMLGNGGSNRSYGFLPGVSGAHHELSHHRGDPAIQAQLQIID
                     TWEIEQFGYLLQKLKTTQDRDGSPLLDSTLVMFSSEISDGNSHGHTDLPVLLAGRLGG
                     KVRPGRHIRKDEPIANLYTSMLGAVGVTNSKFGDDGTGPMQGLT"
     gene            13525..14688
                     /locus_tag="PKL17_03280"
     CDS             13525..14688
                     /locus_tag="PKL17_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43777.1"
                     /translation="MRRSRVLFALFFTFALGPACSDSSTSGTVDGGNPATDGGAVTPD
                     GGSNFASSPVIGGCPLFPSNHVFNTAIDMLPVHPSSATFMTTIGTRNLHLDLGTTTDQ
                     QSATYYGIPWNVVQGNSSPWVAVRYSSVDTSLNWNPRPEADCGNGAAHTLVSPCLATT
                     APSPVLPIPMNPLVEGGIDTTPSQPYGDHHILILDASVCRLWETYHSYPNSQGGWDIF
                     GSATWDLRSNALRAAGWTSADAAGFPILPLLLKADEASSGTIKHALRFTIQSSKIRAQ
                     YVWPARHLTGNGTSQSLPPMGQLFRLKASYQIPPTFNTQAKAILQAMKTYGMYIADGG
                     SDWYIQGDPNAGWQSATFTQVQSVSSSNFEAVDVSPLMSRPGFDPNSATVPPG"
     gene            complement(14712..16637)
                     /locus_tag="PKL17_03285"
     CDS             complement(14712..16637)
                     /locus_tag="PKL17_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HNK43778.1"
                     /translation="MAKLGPGAKVGDQFHLIRKIGEGGCGEVFLAEQTTLGRHTVVKL
                     MHPELSRDASYVERFRREARLAARLIHHHTAAIYAFGETPDKVLWIAMEYIEGESLHN
                     VLARESPRQIADLLNPVADVLDRAASMGIIHRDVKPDNIMVAPDGDGRRAVLLDFGLA
                     RPMEDNDKLTAVGVAVGSPTYMSPEQVFGPTVDAATDRYALGVILYIAVAKRPPFKHE
                     DRVQLVLMHKRNPVPPLNTFVPSIPLGHPLDQFFQKAMAKQPGDRFPSARTYVEEFVK
                     AMDAQPLPIFGETGRASTSGLRQAEHRPASPPPTAQPAQSPPPPPPRRPGAPPPPVPG
                     AIRATQPPAAAAQLGKIEPMQPAQQALRSTMPAMAAIDVSARPDAPARTEPANARSIE
                     GWGARKAAPAAAPQSAPVVAPQPAQAEAGSSAQGHLRLLAFVGNSGEAIDILSAQQVL
                     LGKHRDCDVVCRAFPSPDNDSITHGVSRQHARLLVVNGKLLIEDLRSLNGTFVDDRRL
                     ALGTQVSLSDGQLIRLGPVLTLEVRILRGGAALLRRVDEYHGNFPSTLVTWKEFALGD
                     PVIGLVPPSSDSAVRWGSIRTHGEGHDLWWYGPQAVLWQRGGRSISGAQAILPGDRLT
                     LGATTIEWDEGQKPGLT"
     gene            complement(16650..17021)
                     /locus_tag="PKL17_03290"
     CDS             complement(16650..17021)
                     /locus_tag="PKL17_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNK43779.1"
                     /translation="MKKSDLVSLLADKHKLPRNKTQELVEDLFAELAHALKRGELVDV
                     RGFGTFRIRLSKARLGRNLHTGGTVPIPPRRVPSFRPGKELLTRCNSEQTGEDVSDEA
                     LQGAQPSTHLLRRGKLDSKTL"
     gene            complement(17073..17393)
                     /locus_tag="PKL17_03295"
     CDS             complement(17073..17393)
                     /locus_tag="PKL17_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNK43780.1"
                     /translation="MPRITFLPQNKTIDCAPDETAFVAARRAGILIPTACVGRGSCGL
                     CRVKVENGEESLPPVSKAEKFHLGNTYFITKLRLSCQLTTSGDVTLLIPDAVGKKLPT
                     LPGK"
     gene            complement(17466..18485)
                     /locus_tag="PKL17_03300"
     CDS             complement(17466..18485)
                     /locus_tag="PKL17_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43781.1"
                     /translation="MRRHLLALFLPWLVAAAPEPVDEAKAIVAERDQLIEKIVRGDDF
                     EKSVARFGELKKRFESLSAAKQLSAARDAASSTKAREKWNLYLRSLDYRVGDQCAMSV
                     DPKNRRKAHGYELFRAEYGKIIAKKIVKIPAKTGFDEDESVETYQIGAQTGALSFGAK
                     EMRTHDGKPFSGSVGDWVFLCFASVSSHGSGSYLPPEFRDKVVGSGFASRIKSPPKIA
                     SKAKWNPLHLVGLSTLRSVAQTGRWPIEENVPILSHLIVEKDLGNGRFEIRLDQASTM
                     GRPSQQTFLLDVPSGLRYRDTLGPGEAFWAIMSNPVIDKQLRKLILTAEDLEPFYVEG
                     LDDPK"
     gene            complement(18498..19880)
                     /locus_tag="PKL17_03305"
     CDS             complement(18498..19880)
                     /locus_tag="PKL17_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNK43782.1"
                     /translation="MRQLLPGRWGLGLLLWLVGLVLVPSVVVAKPQPTIAVMPFRDLT
                     GKGKPHIGEAIREVVASDLKSLAGVRIVERGNLDKVLKEMRVQAQLKEVEDDTAAKLG
                     KVVGASVMVIGAYQEESAQIRLTARFVRVETSEIIGSAKVDGSVKQFFRLQDRVTAEL
                     LRSAGMPELARKVVDGTEARPDLKTIEALDLYGQAVTAENDQQKLVLLSSAVAIDQNF
                     SYAVKDLAALEERIQKYQAEQKRLTDAKITELRKDLATTTERAKLDALTDQLLRLLQG
                     SRRYHALVREARLYLEGLPPGPVSQREFDSASVMLVSTDLLLRDYDSVLRDGEVFMQR
                     APSSAMFESVRQMVSQAIDSKRQAEEGKQKLAEYLQKFPNLIGWDLCTLASVYSSHRQ
                     FKEAQRLFDACIKVSDKPAVSHLQAVVMVDMMLGDYAAMQRHIDEAEKLEPSAGRSLR
                     NSYAIQMPVD"
     gene            complement(19912..21180)
                     /locus_tag="PKL17_03310"
     CDS             complement(19912..21180)
                     /locus_tag="PKL17_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNK43783.1"
                     /translation="MKKIGLTAWILIALVLGSSLGAYLGGHLGDGLHPPAWLPKAASF
                     EWMSKTFIRLIKMLVSPIIFSTLVVGLAGAGEKHIGRLLVKALLWFWLATAVALVVGL
                     GIANTLKPGLGAIGGTGSFPTPASKPFYEQVVPTSILDAMAQNAILQIVFFSLLFAMA
                     LVAIGDRGKPVLDVLRGIADAMFKITEYVMRFAPFGVFAAMVVAVGTQGPGVMVQLGK
                     LVLSLYLALICFVALLLVAVKLITGAKMGLILRELREPLLLAFSTTSSESALPKAIAT
                     MERLGVPSHIVGFVLPAGYSFNLDGSTLYLALASLFIAQATGKYLSPGTQIEMMLTLL
                     VASKGVAAVPRASLVVLASTCAAYGLKVEWIATILGVDAVMDMARTMVNVLGNCMASI
                     IVGKWEKVIPQDAPLYTGVLPDTAHSSSDA"
     gene            complement(21177..22160)
                     /gene="hemF"
                     /locus_tag="PKL17_03315"
     CDS             complement(21177..22160)
                     /gene="hemF"
                     /locus_tag="PKL17_03315"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNK43784.1"
                     /translation="MTTQKSSGALGGNEKLAAQSLRPAPQGCDLDGLADEASEWVRSL
                     QRDICAAIVRVDGSASFRFDSWQRPGGGGGLTGILDDGAVFEKAGVNTSVVHGELSPE
                     MQSALPGDGARFFATGVSLVLHPQNPYVPTTHANFRFLRRGRTAWFGGGADLTPYYPF
                     VEDAAHFHSQLALACDKHDADYYPRFKAWCDDYFFLRHRQETRGIGGIFFDYLQADLP
                     TVQSFWRDAGSAFLPAYLPIVERRKDTPFGERQRRFQLYRRGRYVEFNLLYDRGTVFG
                     LRTDGRVESILMSLPPLVRWEYDFRPEPGSEEARLSDFLRPRDYLREMGLR"
     gene            22244..23122
                     /gene="mtnP"
                     /locus_tag="PKL17_03320"
     CDS             22244..23122
                     /gene="mtnP"
                     /locus_tag="PKL17_03320"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNK43785.1"
                     /translation="MSARRVIGVIGGSGLYELSGLTGVHEQTVETPFGSPSDSLVIGD
                     LDDVRLVFVPRHGRGHRLTPSEVPYAANIHALKQLGAEYVLSVSAVGSLREEIHPGHV
                     VLPDQLIDRTKDRRSSFFGNGVVGHISFAEPFCHDLRNVLLGATQDCGTKVHPRGTLV
                     VMEGPAFSTRAESHMYRSWGAHIIGMTALPEAKLAREAELCYATLALSTDYDCWREEE
                     EAVTVDAVVAVLKRNVSLAREVVRRAAQLIAKLPPRSCGCARATEYAVMTHDIAPRDH
                     ERMKLIIGRHIPPPTK"
     gene            23165..24103
                     /locus_tag="PKL17_03325"
     CDS             23165..24103
                     /locus_tag="PKL17_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNK43786.1"
                     /translation="MSSQLDLLVVGSVALDTVETQAATRTEVLGGSASYFSAAASFLT
                     SVGLVGVVGDDFPDEHAQFLTRLGVDSQGLERRDAGKTFRWHGRYSDDFNKRTSIDTQ
                     LNVFADFEPKLHPAHASARLLFLGNIDPTLQLRVLEQMKSVELVGLDTMNFWINGSRE
                     ALLKVLRRVDFLLLNDEEARLLSGRHNLVHAAQAIQRLGPKNVVVKRGDAGALLFHDE
                     HIFAAPALPLTDVVDPTGAGDSFAGGFMGFLSRTWKGGNLDGMLVRRAMICGSTMASF
                     CCEDFSLDRFRSITHPIIDSRFRAFRQLTHFEDLVL"
     gene            24104..24865
                     /locus_tag="PKL17_03330"
     CDS             24104..24865
                     /locus_tag="PKL17_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNK43787.1"
                     /translation="MARTQPKLTGVLPRRYFGTEWQPPSRVDEEAALALAQPAAIVEL
                     LRPLVDEKRAARIDEVTAGRLSGVIVVLEDLHDPHNGGAVLRSSEAMGVSEVHIIASR
                     ERFRVSDKVTQGCDKWLDVMEHHSTASSLSALRQRGFRLAAAVPGVATQIHDLDPLQP
                     TALLMGNEHAGLSAEARALCDVEFAIPLHGFSESLNLSVATALCVFSLTSRRRAALGR
                     AGDLDDAALTELRARFYLRDVRGSLAIVRNRLGAH"
     gene            complement(25643..26617)
                     /locus_tag="PKL17_03335"
     CDS             complement(25643..26617)
                     /locus_tag="PKL17_03335"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNK43788.1"
                     /translation="MLSALIPYPQLPSLPIIDDSLAIHPFGVLVATGILVGAWLTIQR
                     GKFLGLDEEKVKNMIFWSLLTGFLLSHFADVLVYQKHPTTTAMLKALADPRSGLSSMF
                     GFAGAVLGLFLWCRQNGERVLPYADSLAYGLATGWFFGRMGCYVAHDHPGELLLTSDP
                     LYFLGVDYPCPQAHCAPRGTGLFHIGAEFRRHDMGFEEALFAAALAGGYFVWGLFKPR
                     LGVFVASIATLYGPIRFALDRLRVRDGIGADPRAFGLTPAQYAAVLVTLIGLVLWFYV
                     AKEKSVPAVAAAKIDASESPEASAVSASSPVADAAGDDKAEHKSGPVA"
     gene            26859..28184
                     /locus_tag="PKL17_03340"
     CDS             26859..28184
                     /locus_tag="PKL17_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK43789.1"
                     /translation="MKRSTVVLLLLSIVSLLLTGLGLSYRLLRLGLEAAQTSTEQRLG
                     AIGMTLAEALRRGSDTTLLGPVAHDSQLEAAYLLDQSLRPVEDKHTVSLLRVDPDRAL
                     RALRGQPQVGLAYRLELDDEPDNSEPKQSPQILAGYFPVQRRGVVQLLVLEAGERFTN
                     LPERLRRSAMASAGVAAILGALALVLTLQVLRSAARQQRLQAEAQRGQAIRQMAASVA
                     HELRNPLGTIRAGAELLREQAANVELLDDMLAEVQRLSDLTTHFLTFSRDAPLQVQDV
                     DVVALCSEVCSSLRMRFPHPELSIEAPPDGQSICLTADGAKLRQVLLNLCLNAIQAMG
                     ERGRLQVTVRTLRHDVVEILVCDSGPGLSDEASAHLFEPFFTTKTQGTGLGLLVCRQI
                     IEKHGGKLRLVPPGERPAGVPTGACFAVDIPIRSDARGTENAALDVSHK"
     gene            28190..29602
                     /locus_tag="PKL17_03345"
     CDS             28190..29602
                     /locus_tag="PKL17_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK43790.1"
                     /translation="MRHILLVDDEPKLGKMLATALQSAGYTVDRAMTGGEALKKLASE
                     PFDVVITDLKLPDMSGMQVVQRVRELSGAPDVMMMTAYATAETAVEAMRLGAVDYLIK
                     PFSIDELRIKISRLIGRRMLQEQNGNLQAQLEKAQSIESLVGTSARMREVMEQVQQVG
                     PTDATVLLLGESGTGKTALARAIHQQSRRRNGPLCEVHCAALPSTLLESELFGHERGA
                     FTGAVEQRLGHIEKADRGTLFLDEIGEIPPAVQVKLLRFIQDRTFHRVGSSQARSVDL
                     RIICASNRNLAQAVKDGSLREDFYYRINVFPIHLPALRERSDDIPKLVQDILRRRHPS
                     VTVSQPALDLLQRYPWPGNVRELENVIERAAILVGTDRPHGENVITAEHLPRSFVGSV
                     LPVTTSGLLQPGFSIDRLERDLIWEALARTSGNKTEAAKLLGITRRRLYSRLKSIEND
                     AEAEENPSDADPNKTAPGSS"
     gene            29599..30243
                     /locus_tag="PKL17_03350"
     CDS             29599..30243
                     /locus_tag="PKL17_03350"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNK43791.1"
                     /translation="MTDTKTPTPVVPYKSHPWHGISVGDRAPEIVSSFIEMVPTDTVK
                     YEVDKRSGHLKLDRPQKYSSQCPAPYGFIPQTYCGTQCGSYAGDKTGRSEIVGDGDPL
                     DICVLSERTINHGGVLVNARPIGGYRMFDGNEADDKILAVLCDDPAYGAFRSIADCPR
                     SLIERLRHYFLTYKDMPGQNSRRVEITDIYDADEARHIIGLSQADYHANFRPTW"
     gene            complement(30257..30916)
                     /gene="alkB"
                     /locus_tag="PKL17_03355"
     CDS             complement(30257..30916)
                     /gene="alkB"
                     /locus_tag="PKL17_03355"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017131189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HNK43792.1"
                     /translation="MTLPLFGPDSLRQSPRKAEELLPGAWLFPDFALPVMDSVWQALM
                     QVIQLAPLQKMQTPKGLWMSVAMTSCGSVGWISSRSGYRYSACDPQTGRSWPPIPDAL
                     CSLSQSAASRAGFDRFVPDSCLINHYEPGNKLSLHQDRDEQDFTQPIVSVSIGVDATF
                     LLGGFVRTDATRRVRLSHGDVVVFGGASRLRFHGILPIAHSHHPLTGNSRYNLTFRKA
                     C"
     gene            complement(30924..33797)
                     /locus_tag="PKL17_03360"
     CDS             complement(30924..33797)
                     /locus_tag="PKL17_03360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK43793.1"
                     /translation="MLRKDAVWSLGRALFLCGIGLLIRSGRFRIGQWCEHRFEVPSAL
                     GSAVAAGIASVCLLSALWQTRHLIRKLVRVCIAKIDAIDDRLFSSLSTVRARWSRAII
                     VGLLVGIAANAIIETTPIEQGLEASFVDRAYRLRFPRRTHAEVATSTDEPDSAGRLDE
                     VVVIAIDDDTIARLGWPLPRHQYSRLIERVWESHPASLSFDISLLDPSIDHPEWDSLL
                     GDTAARAGSVYFTYSLSSHSRELRLLPTESAMEVLDRNVVPWNPSASVLPEFSLLMGR
                     EVMPRMVIDPIATRTHGVALANVMLDGDDVLRHAVLILRHGKRLLPSLSLRVAADALG
                     VPLSQIRLTPGEEIDLGGKRKIPIDDLGRTLIRYHGRQGAEGGSPVRYVPLWSLLRSE
                     QLVTLKDNPMGDDHEYLIPDNFPDGKKLKPGTQIEGLLHYFTDPGNMIDLSIVSDSQR
                     ESVFDVVDESRMHFVAKIGKVRRRGGVDPGLSGKHVFVGATALATSDFHSSPLGAIPG
                     VEYHATMLANVLRDDFFRTAPLLLRIVLTFIAAISAGLIGALLSPGLGFLVLSLGLLI
                     LLLTVFFSFDSGLHIPVVAPLAALGFSYGICVLLGFRSESRARARAESAKEFVRQTFG
                     RYLTDEVAQQILDSPDGLRLGGQRRFVTVMMTDLRGFTTMCTELSPEQVVKLINHYLE
                     VMTKIITRYHGTIDEFIGDAILVIFGAPLSHGDEERRAVACALEMQNAMAAVNHWNVE
                     HGLPKVEMGIGVHSGDVVLGNIGSDLRAKYTAMGTTVNLTSRVESYTVGGQVLISEVT
                     RQRCGDSVLLGQSQTLTPKGVKGTLVVHEVLGISGPYCVALEPVPETLHRLPVPLSIR
                     FAFIVEKQVGELSAHGTVEALSDVGMELRTGETLATNGNLLLRMVRPQSADDLLAGDL
                     YCKVLRELAPGLYYLRMTAVPMELRATLAAAMSHSLPAENVVK"
     gene            complement(33797..34987)
                     /locus_tag="PKL17_03365"
     CDS             complement(33797..34987)
                     /locus_tag="PKL17_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNK43794.1"
                     /translation="MIRHRFIGLAVSLLVFGFTLSGHADEPCGGIKIQGGMVVLGQPL
                     NSDFIGKDAGRRCLSSVAALLVRNRLMRAVTVSYRADDAVRMDGTALSVARKVADLLV
                     RAGLPQNRVFAVAPSVPSGQNASLTIHYTERAPDSVVARIAKLSGAVSIGFSETALRP
                     AEPSMPILVEDLLRTGPEGVAEVELRDGSGIRIKKESQVRFVKLDFAESGERSVRIEV
                     QRGQIEATVKKAAAGSSFDTSSRIAVASVRGTSFRFGNTTDGKSKLETLTGQVALGPE
                     THGNPMLVDAGFGSRVDQAGVVEKPTPLPQIPRVIAPLVGTLPKDESLSFAAVPSAVF
                     YRVELARDPDFLVEASETEVPQPRLHLSPRPPVGKWFWRVSAVNAAGFASASSPVYSF
                     SVLP"
     gene            complement(34984..36768)
                     /locus_tag="PKL17_03370"
     CDS             complement(34984..36768)
                     /locus_tag="PKL17_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK43795.1"
                     /translation="MSLSVRLGFPLRRWWCLCGLICLSVPQPAHADSARLGFQLSRGA
                     PTSAGSWQFLVERPWYAPTQRFAVGLTFAYDHNLLRLPDSDGPLVAHSLLGSLDVAVS
                     PLRWLLIRANLPLSIYESGTADPTSGDVPIEKPALGDPGFGVTARLYGDIERDRVSLH
                     AGIDGWPGMGQAAGQRRDGLPRHEGDADGRILLPRLVIAGIFAESGRFTVDSGFLYRP
                     VAAIGSPGHTVVAGSEVQIGGALGFAPPGGRLYLGLETRLRARVTGYDVSDKDIVHLD
                     VLLSGQTLLWQKFQLGAAIGTTLFGVGSSVSPQLTTLLRLALAPRHGDRDQDHDGIPD
                     SQDRCPYEPEDKNGYRDDDGCPDALLAPPIVEPASASVTAPESAPSSVAAASSKLSPA
                     NDALVPNALLDSDGDGIPDREDLCPLVPEDRDGFEDEDGCPELDNDDDGIPDAQDVCP
                     LEAETVNGRLDDDGCPERAQAKIDIGTNQLILKEQFLFRPNSAQLDPRSGPLLRELVQ
                     LLRDHPRLAIEIQGHTDNVGGAKRNLLLSQQRADAVRTHLIKNGIAATRLRAVGYGAD
                     KALVPNSSAKNRAVNRRVELWILSGGMP"
     gene            37016..38077
                     /locus_tag="PKL17_03375"
     CDS             37016..38077
                     /locus_tag="PKL17_03375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK43796.1"
                     /translation="MSEAARIGETIGKGRFKLQRSLGRGTFGDVYLAESLDPQATAGQ
                     REAVIKVLHAQWAKVPEVVERFRRESLVTQKIDHPNVARVFLHGELDDGVPFIAMEYL
                     PGKSLRDRQEDGAIPYEVAMDILISIADALSAAHRAGVVHRDLKPENIQLISRNGNPD
                     YPVVLDFGVAKFLDAAEKLTMTGALLGTPAYMSPEQFRGESNLGPPSDIYALGVLGFE
                     LLAGRLPFAGRTFAELAVAHTTQPAPPLTGVPQPLTALFERLLTKEPQRRPRADLLMQ
                     SLKSIKDGSPPPKLPEVFVDPYAATGRLDEAVRTAVISQAAMERKLQKRQQLYTILLA
                     AAVFVFTCTIAVVIYMFLL"
     gene            38096..39229
                     /locus_tag="PKL17_03380"
     CDS             38096..39229
                     /locus_tag="PKL17_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNK43797.1"
                     /translation="MFDDRRLSLLLFRVLCYVVLLVIAYSVLSRLSLVLTPVSVALVL
                     AFLLNPSVSALEERKVPRWLAVGLMLLFLLGVLTAVGVTIPYLVRSEIERFSAQLPSY
                     QGLIDRKVLPLLGKVFKYRATSIGDWLKLLTNQVTEWVRGAMGQISDALGTAILSVAG
                     LFKYLVAGLLIPVFTVSFLMDMPAIGENLKVLVPPRHKQLVVDIMDDIYGALGSWLRG
                     QLTVMLIQAALYSIGLSIAGIPLAIFIGCTAGLLAFVPYVGVMIGLCSALLVALLEVS
                     TAGATPILGVLATFGAVQLLDALVITPRAVGGRIGLSAVGVIFALSLGGSLLGYAGLL
                     LAVPFAAVFKALWPRLKDAYTGTAFYRGEAVADPVAAKTSQSE"
     gene            39237..40046
                     /locus_tag="PKL17_03385"
     CDS             39237..40046
                     /locus_tag="PKL17_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNK43798.1"
                     /translation="MTTTDSPETDLLVSTQGAVRILAMNRPQTKNGLTLELLSALLDA
                     LKAAENDPAVRAVVLTGRGDAFSSGLDFRAAMHLVQSGGSDFAEQSENRIRSYFHAVI
                     RALTALPKPVIAYLDGVAAGFGCDLALACDLRIGTKKARFGEIFIKRGLMPDGGGTYT
                     LPRLIGLGRAFDLMFTGDIVEAEEAQRIGLLTRIFDGEAEALAFAERIAAGPPLVMAE
                     IKKSVYAGLGQSFSDALENEVAGQVKLLRSQDFIEGVSAFLMKRPPNFTGQ"
     gene            complement(40050..>41731)
                     /locus_tag="PKL17_03390"
     CDS             complement(40050..>41731)
                     /locus_tag="PKL17_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43799.1"
                     /translation="EHVPEPIQLSLKLVPSLSPRLDLVELAGPQRTTGKIHPNDWLIV
                     RGDGLLLGGGEGVTVALLSGCFLPEGASEPCSANGKTVREVEIPVQPQSRFDRTNGVL
                     PYSPTIHGVMPGKFVGTILLRNQPPGTTMKPVSASRTLAVTQVRPELTGVAPSAASLG
                     QYVEMQGAGFIGGQPGQATLVRLQGQFSLENSPVMIPVDTEIVLKWLARFPSPVGRYV
                     LDETDALGQALESRGGLREAAGVFVGTGTMTLRNGKETVASKPTPLRIALLRPKQVVV
                     VRFLSSYQDSLRLFGLRAVDNAVRARVLEVARRDYAGINIEFREGGEDMPLPEDFAYY
                     AQVDIGGPDPNGLGYLGYDNTPGRDMQNRRLYDRIGGVNAVTQNDGAPGYGGVFAEQL
                     LGFSNHPGSVAAIKSGPQEADLFDQVFDPLRPDTGGNPATLREATLLPALSSGSLCPA
                     SKDDRPHQVACGIFVLGSLIGTTMTHELGHSLGLANPDNRNGSYHNNGSLRGRIMNPG
                     SLRSFRERAELSPSGPAVFCDTDYAYLRKILPGSALPPSIERPACLDTPAL"
BASE COUNT         8633 a        12694 c        12133 g         8271 t
ORIGIN      
        1 accgccgaag tcccgatctc cgccacggta gccaccaccg ccacggcgat caccaccgcc
       61 gcctccacca ccaccgccgc cacgacgatc gccgccgcca cggtagccac caccgccgcc
      121 gccgccaccg ccgccacgga agccgcccga ctcacgcgct tgggcctcgt tgatcttcaa
      181 cgtccggcca cccagctcgc tgccgttgag ctgcgagatg gcatcacgcg cctcctcgtc
      241 ggagctcatc tccacgaagg cgaagccgcg cgggcggccc gtttcgcgat ccatcatcac
      301 cttgacgtcg gtcaccgcgc caatgcgctc gaagacctcg cgaagctcgg gctccgtggt
      361 gctgtaggaa aggttgccga catacaggcg cttgctcatc tcatcactcc ttgcgttcgt
      421 cctttttcgg tgacggtgtc gtcacgttcg acggagctgc cgtcgcagag gccctgctgt
      481 gtttcgcacc aaccctagct cgaacgaagc ggcacacaga gggcagcgac gcagagatga
      541 aaccaaggca taggacctag acctacttgt accatgaacc tggtccgcgc aagcagagtt
      601 tcgcagtcga ataaataaaa tcgatcgaga cgcagcctgc ccaacccaaa gtgtgaacaa
      661 attttacaaa cttcaggctt gggcgggtgg gggcggaggt ctagattggc gacatgagtt
      721 cacaatgccc tgcccttcgt cgcgcagctc catcccgtcg ccaagcgatc ggatttggcg
      781 ggtcactgcc gcttttgatg gcgtcgatcg gtcttttgtc gtgcacgcaa gatccgccga
      841 ttccagccga tgacggtccg cccgatcttg tttccacagc acccgatctg gcagtgcctg
      901 gggacccacc cgacctgacg atgccgccgc cgtcaccgga cttggcgatc gttccacttg
      961 cgccttgtag ctggcgcgat cgctttgccg aagcgtttgg tcacgccttt gcgaaaaatg
     1021 atgtcgcgtt tgtcaccacc gacggaaaag tgacgcgcgg aatggatcag ctcgactttg
     1081 cggcgcagaa aagccagatc cccgacattg tgaaggtgaa gggcaacgcc aatcgtatct
     1141 cggagattca actgctcgac cctgcgacgg gaaaagtcct tgcatccaac aaagcggcgt
     1201 tcgtttatgg actcggttat gccatgtggc tcgaagtcaa cgcatatggc gaagccggca
     1261 tcaccgagat gacgttgatt cgacagtttg ccggcgagct gccgacgtat cagcagctgg
     1321 ttggcctgta ccgagtgctc accaagacag cagaagcgca agtgccggca gccgatcgcg
     1381 gagactttca acaagcggcg gccaagctga tgagcgcgat cgagagcggg tctttgactt
     1441 ttgcgctggg aaccggacat gcggcggact ttccagcgac ggcgcagacg gccaacggca
     1501 agactttttc gtttcaactc tcgaacggct acggcatgga cccgcagatt gggttccagc
     1561 gcaacggcgt gtgggggccg ctgtggcaga cgtacagtct cactggggcg tggtcggcgt
     1621 cgacgccagg catgcagccc aagtgcggcg gctgcacaac ggacagcttc ggcggcacca
     1681 agtatctgtt ctgcgaaggg ccgctgccgc gcaaaaacgc agctgctgtt tgtgcttcgc
     1741 atggtggata tttggcgacg gtcgacagcg tatttgtaaa ctcgtttttg cgcggcatgg
     1801 cgcaaaatgc gggcaaggcg tttctgggtc tggatgatct agctgagtat ggaaaatacg
     1861 tgtgggcgac gggaaaaccg gccacgtata ccaactgggg tcaggggcag ccggaccact
     1921 atatgatgca agagcgctgt gttcagcttt acgacgggaa agcgatcacg cagtggaatg
     1981 acatcgactg cgcaagccca ctgccttatc tgtgtcagct tccgtaaatc ggaaggagtg
     2041 ggcgcgcgat ggggggcggg aattccgggc ctattcgatc gggttgatct tgtcgaggtt
     2101 gagtccgccc gcatacgagt acgacgtcgc atacgcatag ccatagccga gcagtccaaa
     2161 cggcttggtc gacgtaatgc ggtgattgcc cggtttttcg atgcgaatga tcatccgccc
     2221 gatggtaccg tcggcgattc ctcggaacgg cgtcgtaatc gcttgtccat caagtgacaa
     2281 cttcgcatca ggagccgccg tcacattgat gaagtcatag ctataggtct gcggcacgta
     2341 gaagatgtag tcgtttcggt attgctcgac gggaaccagc ggcgtgatgt tggcatcccc
     2401 gagacacttc acatcatctg ccgtgccaat gaacggacag cgcgccgaca ctcccgacgg
     2461 aaactctttt ggatcgtagc ggcccgttag gttgccccac gctggcatgt actgaaacac
     2521 caggatcggc tgatcggatt cgacgacaaa atcggcatcg gtcgcaaact cgtaaatctg
     2581 acctttgcta agcggcggca ccgccggcag catcgtcgtg ctcttgatcg tcacgttggt
     2641 gttatcgacg ctggcgacga tgcggtaata gtctttgtcc gacagattgc gctgcttgac
     2701 cttggccccg acgtagcgct tgccccaggt ttcaagcgga aacatctgca caccaaggtg
     2761 attaccaccg acggcacttg cgggaattgt gaccccgccg gcgccgccat aaacgaccac
     2821 cggcgcgctc gctttgacca ccgcgcccga gatgtcttcg cggctgttcg cctgcacgat
     2881 ttccatcact tcaagacgct tgagcaggcg ccgcaccgtt tcacccgcgc gcaacgccgg
     2941 cacgcccgga cctttcgccg tggcgactgg cggcgtcact tccaccatcg tgtcgtcggt
     3001 cagcgccatc actgccaaaa gacccgcgcc ctgtggcggg aacgtcatgt gctcggcgtt
     3061 gtacgtgtac gacatgacaa agtagttctt tgccatcaca tgttcaggca gtagcagcgt
     3121 cgcggaacca gaaaacaccg tcgctgcatc gatcgggtgg aactggtaca ccgcgaccgg
     3181 cgccgtcgat ttcaaataga agcctttttt ggcgatctcg gtttcggcca gcttgttctt
     3241 gtcgtgcgac agacggaaaa ttgcgacgcc cgccgccgga ttgcccatgg ctggcggaat
     3301 gctcttggtt tcgatgacgc cactggcatc agacagcgtg acggacgcgg tgagccggtc
     3361 gctcgtgttg gtgacgacaa taccaaaatc accgacgaga gaagttgacc acaagttggc
     3421 cggataaaag ctgcatccga cgttgccacg ggtgacttgt tcgcacggag tcagacactt
     3481 tccgtcgatg catgcatcgc caccggcaat gttgcatttg tcggcaccga tccagctgtt
     3541 gctcatgcac cgctcggtgc cgtcgcggtt ggcgttgcag cgaattcggc cttcgtcaca
     3601 gctcggcgga ggtggtggcg gcggaataga catatccggg gccatgggac cgtcttcatc
     3661 gacatttccc gtcgccggat tgcaagaacc gacaagcccc aagacaacga aaccgagcga
     3721 ggaccgagcc atggtgcgca taagtcatgc ttagcccgaa gatccggctc tgtcacgcat
     3781 tttggcgtac ttgtacgact atttcacacg tagcgaactc tgcttcttca tagaagcgta
     3841 ctctacaaag ctgtgtaaac atcacaactg atcaggtttg ggtgatgcag cgcgtggctc
     3901 gttgggtacg caatgtctca atgctcgacg gggacgccac ttctgccgac atcatcgcac
     3961 actatgcgtc aatctgtgca gatatccgca atcatgaatc agaagtctaa ttctcaatct
     4021 ttttattcga tgtagtacca cgttgtggtc tacaatcatt gagccattgc cctaaacact
     4081 ggcagtccca cggggtccgt agacccggtg agttacgcgc aggcgaaacc tagcaatgaa
     4141 gttttttcga cttatcctcc tcgctctcat ctgttcgctc agcgtcgtgg ggcgctcgac
     4201 ttcgtcgccc aacgtgcgca cggcgtatct gcaagagagc ggcggcacgt cgtttggcaa
     4261 tccgtcgacg atggcggcag tgccggcgcg cgactcgtct cgttttccga ttcagcttgg
     4321 gtcgcgcagt gaagcggtgt tgcgccgcag gctgacgctg tttgttccac cgaattcgga
     4381 aattccaccg tttgagccgt ctcctgctga tttcgagcct gcgcccgccc tggttgaccc
     4441 acgcccacgt tttcgcggtg agctcgacga agaaattctg gcgcggctgt gttcgctgcc
     4501 gataaagagc atcaagccgc tcggtggtgg ttcgtcgatc aagctgcggg tgacgtttgt
     4561 cgacggaagc caagctgcgg tcaagccaaa ccaactgcgc atcacgcgct atcaggccga
     4621 agtggcggcg ttcaagctgg cgcggacgct cgggatcatg tcggtgccgc cgtcgtgcgt
     4681 gcggacgttt agcaaagaac agctccaagc gaacatgccc aaggatctgc gcgagcggat
     4741 ggatcaagag cttgtggtcg acgacaaagg caacgtcaag acagcggtga tccagtgggt
     4801 gccggggctg cgcaacatga agctggaaca ggccgactgg tggcgtccgc tgctgaaggc
     4861 cggcgagccg attccaaacg gcaaacacaa gcgggtcgcg gaaatcagca cgctgcttct
     4921 gtttgactat cttttgctca acttcgatcg ctggtcgggc ggcaatacga ccgaagccga
     4981 cggcgagatg atctacatcg accagggcgc aagctttggg ccggacaaaa agcacggtca
     5041 ctcgcggctg atgctaaaaa cgctgcggtg gagcgagcgc ttcccgcgga cgcttgcgca
     5101 gacgctgttt cagctggacc tgccggccct gcgcagcgac tttggcgact tggtcagcga
     5161 cgacgagtgg gacagcttcc tgtatcgcgt cgagcacgcc cgcgcttacc tcaagtcgct
     5221 caagaaactg aaccacaaag actcgctgtt ttaaccaccg cggactgcaa agtaggaaat
     5281 tgtgccgcgc gggcgtcttt agtaccgtga gccatgctgt cgcgcccgcg tggtcgtttg
     5341 gtgacgttgt ctgccctatc tgttgcgctg ttttcggtcc agctcgcgtc ggccaaacgg
     5401 acagccgaaa agccagtggt cgcgaaacga gcgacggcag aatcggcgcc tgcggtttcc
     5461 gctcctgcgt cggtgttgcc gtttgtcgaa aacgactttg cgctggcccg cgcgcaggct
     5521 ttaaaaagcg gcaagctgct gtttgtcgac gcatgggcgc tgtggtgtca cacctgcctg
     5581 agcatgcgga attttgtctt taccgacgtg cagcttcagc ctctcgcgaa tcggctggtc
     5641 tatgcagcga ttgataccga acagccgtcg agcgcggctt ttttggcgcg ctttccgatt
     5701 tcgacgtggc ccacgatgct ggtcattgca ccgtcggaac aaggagacca gattgtcgcg
     5761 cgctttacgg gagcgctatc ggccaaagag ctgtcgagcc ggcttggcgc ggtgctaaac
     5821 cagcaggcgc agcagcagcc caagctggca aaagccgatg cgctggcggc ggcgggacag
     5881 catcagcaag cggcggctga atacgaagaa gcgctggaag agccaggctc gcggcctcgg
     5941 gcgctgctcg ggctgattca gtcgctgcgg tcgcttggac agcatgcgcg ctgtgccgac
     6001 ttatatgacc gctcatttct gcaagttggc aaaagcgctc tggcgacgga ctttgcggcc
     6061 tatgcggcaa gctgcatgga caaactggat gatgtcgacc gacggcaaaa actgcggcgg
     6121 cagctccgga ccgatttggg caatctgctt gatgatccga gcgcagactt ggccattgat
     6181 gatcgcagcg atgcgtatgg aacgatggta gagctgtccg acgcgctcgg tgaaagcagc
     6241 gaaggcgacc gtctgacaac gaaacgactg gcgcttctgg aaaaagcggc agcgacggca
     6301 gcttccccgg ttttggcagc gacgtttgat gcgcatcgtt tcgattcgta ccgtcggctc
     6361 aagcgctggg cgattgcgga agagatgttg ctggcgtcgg caaaggcgct gcccagcgac
     6421 tacaatccgc cggcccgttt ggcgcgtctg tattacgaaa ccgggcggat tgaaaaggcc
     6481 gaagcccaaa tcggcacggc gctgtcgcat tgtcttggtc cccgtcgggc gtcgatgttt
     6541 gagctgcgcg cagcgattca aaacagccaa aaccgcagcc gcgaggcgat ggcgtcactg
     6601 atggaagcgg tttcggtgct gacggccaat cagcctgccg gtgcgccgga atccgcgcgg
     6661 gtgttgtcgc tgcgccagca actggccgga cttgaggcga agcgcaaaga gcagccggat
     6721 gacagtgacg gagcgcccca gtcggtcccg cctgcgcaac aaaacgcaaa accgggacgg
     6781 aaaaatggtg gccaaaaaac cggcgacagg aaaaacgcgg acaagaaaaa cgacggtgcc
     6841 agcgagcgca agtcaccccc gccgcgttat gaaccgggaa cccccgtggc ccagcgcaag
     6901 cgcgaccgcc gactggccac ccgcgacgcg aacctcaaat agtggcggtt gcggacggct
     6961 ggccgcgtaa gtcacgcaat ttcctctcgt ttcgtattcc gttctgtgcg attctaacga
     7021 gagcttgagg tgttcgcttc ccgacgcaag cgagcatcgt gggcgcaact tgggggagga
     7081 acatcgtgtc agtgtcgcat cttgcgaaag tgagcccgtc gcagcttggc tttgtgcggg
     7141 cgtttctgtg gcagcttgcg cggacgggtc aggtgtccga aagcgcagtc gagcagcaag
     7201 ccgcggtcga cgccgcacgg ctgatttttt cgatttcgcg gagccgagac gagctgtggc
     7261 ggtttttcgg ggaatttcgc cagctggtgg aaagcggcaa cgattcagcg ctcagcgatg
     7321 cgctgcgggc ggtgctcacc ccggcagcgg cccaagaacc ggtgcccgct gaagtgccag
     7381 cgcccgttgt cgaagcggtt gaaaaagtcg aagcgccagc gcctgtcgtt gaaaccagcg
     7441 ccaaagccga agtgccggcg tcggttcctc cggctgcgat gtcgaagagc gctcgtcgca
     7501 aacaggccaa gcaagcggca gatgcgcaag tgacgccgac tgctgccccg ccccccgcga
     7561 cggattcggt gcccgcccaa gttgtggaat ccgcatcaag cgacgacgcc ccgatggcca
     7621 ccgctggcga caccccagat gacgaaggcg gcgacgcatc gaacggcccg tcggcgcacg
     7681 tcatccacgg cagcgatgcc gcaaacgaaa ccacctagca tcacgacgcc gagtacggtg
     7741 gccgtgtcgg cagctcggtt ttgggcgaaa atacgatcga tgcagcctag cgtgcgggca
     7801 caaccgatgc tgccgcctgg aatcggcgtg gcgctggcca tgctgggcgc gctgcttgcg
     7861 agcgcgtgca gttatcgtca cagactgctg gtcgcgatcc aagatccgac gcagtgggcc
     7921 gacgatccga taagccgtgg tgagctgtat cgtcagcttg gcagcgacgc gatgctggcg
     7981 ctttgcgtgg ctgcgatgac atggcttgtg gtgctggcgc tgctgccgct ttcgcggatg
     8041 cgggcgctgc gggtcttggg tctgggactt ctcagcgcgc tgcttcttct gcttgggatg
     8101 cttctgcaag cccagcacgg aacgctgctc gcgatgcgat cgggactgac gctggaactg
     8161 ctgaaggaat cgctcgatcc ggctgcgatt cgcgagtcat ggtcactact gccgcgcagc
     8221 gagctgccct atctgctgat gccgctcctc ggtttttggc tgtcgtggct ttcgctgcat
     8281 ctgcatcgga ttgtgcgcat cggtttcctg ctgtccatcg gcgttgtgac cgtcgcgctg
     8341 ttcacctggg cggtgcgcgt tccggcggca ccgcttcaga ccgcgctgct gcaccatccg
     8401 ctgggttttt tggccaccga gcttgtgtct gcaaagcgct cgcagcatgc gctggcccaa
     8461 gtcggcgtgg gcgtcgcggt tgcaaacgcc gcaacccgca accaagcacc gacagaatcc
     8521 ggcggaccgg ctggaggggt cgatggccca cctatcggag acgatgctgc cgattccccg
     8581 cccgtcgaag aagaaacgca agctgtcgcg ccgcccagcc aagccagacc acagcttgcg
     8641 ctgaccgatt cgatgtttgc ctatctgccc agcgagcgac cgattcagaa agccaaacca
     8701 caggcggtga gcgggccgct gaatgtgatc gtactgattc tggaaagtac cggcgccgac
     8761 tatgcccttc tgccgattca aagtggcaag ctggcgatgc cgtttctgca ttcgctgacg
     8821 gaaaaaggac tgtttctgcg caatcatttt tctgccggaa actcgagtcc acgcgggatt
     8881 ttttcgctgc tttctggtct gtatgtgatg ccggaagtgg gaatctggga tgttcgcaaa
     8941 gacatttatt tgccgtcgct gacaacgtat cttggatcga cgtaccagca ctttttggtg
     9001 acaccggctt cgctcgattg gtacttcccc cacaactatc ttttgcacag cggcttcacc
     9061 gagctttggg gctatcacaa tcttccgatt cgaaaaaacg cccccggtgg ccgcgcccat
     9121 gcccgcgatg aagcggagtc cgtttcggtc ttcctacaaa aactttcgac ggcagcagcc
     9181 aaatccgaac catttgtggc ggtgtactat tcgtttctgt cccactggcc gtacccagac
     9241 tatggaccgg attcccatgt ggttccaccg acgaaagcgc tgcatctgta tctaaacaat
     9301 ctgcactatc tcgacaaaca gatcgaacgc atctacgagc gcgccaaagc gcttggaatc
     9361 ctcgacaaaa ccatcgttgt gatagcgggt gatcacgggg aagcgttcgg acagcacccg
     9421 cacaattaca cgcactcgcg acagagtttt aacgaaaact accgcacgcc agcgctgctg
     9481 ctgcatccgc agctgtttcc tccgcgcgtc gtttcggaac ccaccagcca cgttgatttt
     9541 ctgcccacga tcttggatgc gatgggacgt tcctatgatc cgcaactgct gcagggagag
     9601 tctctgtttc aggaccattt ccgtcgaaaa tacatcttcc tgtatggcaa tgaggacacc
     9661 tcctcttcca tcagtgaaaa ccaagtgaaa ctgcaggtct cgtttcgcga tggatcgtgt
     9721 tgggtgtacg atctgcgtgt cgatccaacc gaacagaaac ggctaagctg cgccccgcac
     9781 aaagcacagt acgaagcgct tttggaatat cggaagcgac aaagacaagc gctgcgcacg
     9841 tacaatgcgg tcttccgccg cgagcttcag caagggaatt ctccgtctgc aatgctggtt
     9901 cctgacgcat cgccagttgg cagaaactaa gcgaacgctt gaaagtctgg ccgcatccgc
     9961 ggtacagcct ccgcacggag gtcggcagcc ctccgcctgg aggaaatcgc agatgtcgac
    10021 ccggttccgc ttcccacgca agacccgcgc gtttctgttt ggtcttatcc cgctgcttac
    10081 ggcatgtgaa aaaacgcctg tccccgtcga tcctgggcgg gtgacgctgc atcggttaaa
    10141 ccgcgatgaa tatgacaaca cggtgcgcga tctgcttggc accagccaac gtccagcgca
    10201 gcaatttccg tcggatgaca gtgggtatgg tttcgacaat gtggcgcagg ttcttagtgt
    10261 ctcggctccg catgtcgaac gctatctgcg cgcggcggaa tcgctgatcg acgaagcgct
    10321 gcgtcccgat ggttttccac tgaacaagct gctgcaagcg ggcgatgtcg aagtccgtta
    10381 tcggcgacgc aatcagatat ttttcgccag caattcatcg attccgatcc gcatcgaagt
    10441 accgacaagc gcaaactacg tgatcagcac cagtgcgttt ggtgaacaag tcccaccgga
    10501 ccccgcacag atggtcattg ccgtcgatgg cgtgctcgtc aagacgctga atgtaaacgg
    10561 aacggaatcg gcaccggatc agatccagat cgaagttccg ctgaccgctg gaacccacat
    10621 gctgagcttc ggctttgcca atgatctgta tgtacccgat ccagatcccg gaaagaccaa
    10681 ggatcgcaac ctgggggttt actggtttcg cattcacggt cccgttggtg taaatcctga
    10741 aaatgccatc cgtcgccgca cgatcacctg cacacccagc acgtccgatc aaaacgaatg
    10801 gcagcgctgc gctgatcaga ttctgtcacg gttcgcgcga cgtgccttcc gacgcactgt
    10861 atccactgaa gaagtgacgg aactgcgcgc tctttcgcag gaagcttggc gcgcgggtga
    10921 tgactttctg accggcttgc gtccggccct gatcgcagtt ctcttgtctc cgcgcttttt
    10981 gtatcgggtg gaaattgatc agaatccatc gtcgctgacg ccgcatcggc taaacgactt
    11041 cgagctggct tcgcggctgt cttactttct gtggagcagc atgcccgatg atgagctctt
    11101 gcggctcgca gagcagtctg ttctggatga tgacgacagt gtgctttctg cgcaggtcgc
    11161 acggatgctc gatgatccga aagcagaaaa tctgctgcat cgctttgctt cgcagtggct
    11221 tcagcttgca acgctcgacg gagtgacccc tgatctgaag cggttcccaa cctggaatga
    11281 aagtctgcgc agcgatatga agcaggaaac actcctcttt ttgcgcgact ttatgccatt
    11341 gctcggcagt cggaatctgc ccatcacacg gctcctcagc gaaccgatga cgtatctttc
    11401 accaacgctg gcgaagcact acggaatgga tggtccgggt ccggatgtgc cgtgggaaca
    11461 gacgacactg ctggggacac cgcgcatagg acttttgaca caagggagtg tcttgaccat
    11521 gacttcctat ccgggacgga cttctccggt caagcgcggc aagtggattc tggaacagct
    11581 cctgtgctcg cctcccccgc cacctccacc tggtgtatcc accgatttga ccgcgatgat
    11641 gggaaacgga acgctgcgac aaaagttgga agcccaccgt cagaaaccag agtgcgcggg
    11701 ctgtcaccgt ttgatggacc cactgggttt tggcctggag tcctttgatg cagtaggtgc
    11761 attccggaac accgatgaag gccagccgat cgacacaaca ggacaactgc caagcggcgc
    11821 aaccttttca gatcacaccg agctgaccga gcttgtcaaa agcgatccgc gctatctgcg
    11881 ctgcatcgca atgaagctgt ttacctatgg gataggtcga actcccgtcg acagtgacga
    11941 aacccagctc gcaaacatca tcgaacaact gggatcaggc gaagggactt tgcggcagct
    12001 cattttcgcg atagcaaagt ctgaaccgtt ccgttcccgc cggggagaga ccgattccgt
    12061 ggaaggagtc acgccatgac cgctgtcacg tacccatccc gccggcagtt tcttcgcagt
    12121 ggcggatctt ttatcgccct tccctttttg gaaagtctgc tacccaagac agcgcgtgcc
    12181 gacggaaaat cgccgcagcg gctgctcttc tatttcgtgc cgaacggcat ccacatgccg
    12241 gcctggacgc ccagcagcga aggcacagac tatgtgattc cacccatcct aaagcccatc
    12301 gaagcgctgc gtagcgagct gttggttgtg tccggacttc gcaacttgcc agcccgtccc
    12361 gacggagccg gtgatcatgc aggcggaacc agtgcatttt tgacctgcgt gcatgcgaac
    12421 aaaagtgaaa cggacgtgcg gctgggaatt tcggtcgatc aggttgccgc ccagaagctg
    12481 ggaaccggca ctcgctttgc ttcattgccg atcggaatgg aaggcggtgc gtcggtgggc
    12541 ggctgcgatt ccggttattc ctgcgcgtac agtcagaaca tttcgtggat cggacccaag
    12601 actccgctgg ccaaaattgc cggtccacag cttctgtttg atctgttgtt tcaagacagc
    12661 agccaaggaa caggagtcgc ggaaaaacgc ttccgtcatc gtcagagcgt gctcgatttt
    12721 gtccgtcggg atgcaggatc tctgcggggc aagctcgggc gcagtgatcg cgacaaactc
    12781 gatgaatatc tgcactccgt tcgcgatgtc gaacagcgca ttcaaacgat gtctggaagc
    12841 tgtgatacgc cgatgccacc gacggatgat gtgcgtttgg gagaacagtt aaaagccgtt
    12901 tcggatctga tggtgctcgc gctccgctgt gacctgacgc gcgtgcttac gttcatgctg
    12961 ggcaacgggg gttccaaccg ttcctatggt ttcttgccgg gtgtcagcgg ggcgcaccac
    13021 gagctttccc atcatcgcgg cgatcctgcg attcaagcgc agctgcagat catcgacacc
    13081 tgggaaatcg agcagtttgg ctatctgcta cagaagctaa agaccaccca agatcgcgat
    13141 gggagtcctc tactcgacag tacgttggtg atgttttcga gcgaaatttc cgacggaaat
    13201 tcccatggac acactgactt gccggtgctc ttggccggac gtttgggtgg caaggttcgt
    13261 ccgggtcgac acatccgcaa agacgaaccg atcgccaacc tgtacaccag catgctaggt
    13321 gcagtcggcg tgacaaacag caagttcggc gacgacggca ctggcccgat gcaaggactc
    13381 acttagccgc ttgcacttcg tccgcggatt tctgggcaga accgcgacca tccacgcaag
    13441 gactctccgc gcaaatgggt tgcaggtatc atcgcatgtt gcgcacgcat tcccgtttgc
    13501 gcattaccct tcgcggaggt caacatgaga cgttctcgcg ttctcttcgc actctttttc
    13561 acgtttgcac taggccctgc ttgcagtgat tcttcgacga gcggaactgt ggatggcggc
    13621 aatccagcaa ccgacggcgg tgcggtgacg ccagatggag gaagcaactt tgcatcgtcc
    13681 ccggtgattg gcggctgtcc gctgtttccg agcaaccatg tgttcaatac agcgatcgac
    13741 atgctgccgg ttcatccaag ctctgcgacg ttcatgacga cgatcggaac gcgcaatctg
    13801 catctcgatt tgggaaccac tacggatcag cagtctgcga cgtactacgg aattccctgg
    13861 aatgtggtgc aaggaaatag cagtccgtgg gtcgcggtgc gctactcttc cgtcgatact
    13921 tcgctcaact ggaatccgcg tccggaagcg gactgtggaa acggcgcggc ccatacgctt
    13981 gtgtctccgt gtcttgcgac cactgcaccg agtccggttc tgccgattcc gatgaatccg
    14041 ctcgtggaag gtggaatcga caccacgccg agccaacctt acggagacca tcacatcttg
    14101 attctggatg cgagcgtctg tcgtttgtgg gagacgtacc actcctatcc gaacagccag
    14161 gggggctggg atatctttgg ttctgcgacg tgggatttgc gatcaaacgc actgcgtgca
    14221 gccggctgga ccagcgccga tgctgccgga ttcccgattc tgccactgct cctgaaagcg
    14281 gacgaagcga gttccggaac gatcaaacat gcgctgcgct tcacgattca gtcgagcaag
    14341 attcgcgcgc agtacgtgtg gccggcgcga cacctgacgg gcaatggtac atcgcagagt
    14401 ctgccgccga tgggtcagct attccgtctc aaagcgagct accagattcc gccgacgttt
    14461 aacacccaag ccaaggcgat tttgcaggcg atgaagacct acggaatgta catcgccgac
    14521 ggtggttccg actggtacat ccaaggcgat ccaaacgccg gctggcaaag cgcgacgttt
    14581 acgcaggtgc aatcggtatc gagcagcaac tttgaagcgg tcgatgtatc gccgctgatg
    14641 agccgccctg gcttcgatcc caactctgca accgttccgc ctggctaatc gccacgcccc
    14701 ggcccgccgc gtcaggtcag gccgggcttc tgtccttcgt cccactcgat tgtcgtcgcc
    14761 ccgagtgtca aacgatcacc tggcagaatc gcttgtgcgc cgctgatgct ccgtcccccg
    14821 cgctgccaaa gcaccgcttg aggaccgtac caccacagat catggccttc gccgtgtgtg
    14881 cgaatcgatc cccagcgcac cgccgaatcg gagctcggag gcaccaggcc gatgaccgga
    14941 tcgccgagcg caaactcttt ccaggtcacg agcgtcgacg gaaaattgcc gtggtattca
    15001 tcgactcgac gcaagagtgc cgccccaccg cgcaaaatcc gcacttccag cgtcagcacg
    15061 gggcccaggc gaatcaactg accatctgac agcgatacct gtgtgcccag cgccaagcgc
    15121 cgatcatcga caaacgtgcc attgaggctg cgcaggtctt cgatgagcag cttgccgttt
    15181 acgaccaaaa gccgcgcgtg ctgccggctg acaccgtgtg taatcgagtc attgtccggc
    15241 gatgggaaag cccgacaaac cacgtcgcag tcgcggtgtt ttcccagcag aacttgttgc
    15301 gccgacaaaa tgtcgatcgc ctcgccggaa ttgccaacga atgcgagcag acgcagatgg
    15361 ccctgcgccg aacttccggc ttctgcctgc gctggctgtg gtgccaccac cggtgctgat
    15421 tgcggagccg ccgccggtgc cgctttgcgc gcgccccacc cttcgatgct gcgggcattg
    15481 gccggttcgg tacgcgctgg tgcatcgggc cgggccgaaa catcaatcgc cgccatggcc
    15541 ggcatcgtcg accgcagggc ctgctgggct ggctgcattg gctcgatttt gccaagctgt
    15601 gccgccgctg ccggtggctg ggtagcgcga atcgctcctg gcaccggtgg tggtggcgca
    15661 cccggacgac gcggtggcgg cggcggtggc gattgcgctg gttgcgccgt cggtggaggc
    15721 gaagccggcc tgtgttccgc ttgccgcaga ccgcttgtcg atgcgcgccc ggtttcaccg
    15781 aagatcggca gcggctgcgc atccatcgct ttcacaaact cttcgacgta ggtacgcgcc
    15841 gacgggaaac ggtcccccgg ctgcttcgcc atcgcctttt ggaaaaactg atccagggga
    15901 tgcccaagcg ggatgctcgg cacaaacgtg ttgagcggcg gaaccggatt gcgcttgtgc
    15961 atcagcacca gctgaacccg atcttcgtgc ttaaaaggag gccgcttggc gacggcaatg
    16021 tacaaaatga cgccgagcgc gtagcgatcc gtcgcggcat ctaccgtcgg accaaacacc
    16081 tgctccgggc tcatgtaggt cggcgaacca accgcgacac cgacggcagt cagcttgtcg
    16141 ttgtcttcca tcggacgcgc cagaccaaag tcgagcaaga cagcccgccg tccgtcgcca
    16201 tccggagcaa ccatgatgtt gtccggcttg acgtcgcgat ggataattcc catcgatgct
    16261 gcgcgatcga gcacatctgc gacgggattg agtagatcgg caatctgccg aggcgactca
    16321 cgagcgagca cattgtgcag gctctctccc tcgatgtatt ccatcgcgat ccacagcacc
    16381 ttgtcgggcg tctcgccaaa ggcgtagatc gctgcggtgt ggtggtggat aagccgcgcc
    16441 gccagtcgtg cttcccgtcg gaaacgttcg acgtaagagg catcgcgcga cagctccgga
    16501 tgcattagct tgacgacggt gtgtcgtccg agggtggtct gttcggccag gaaaacctcg
    16561 ccacagccgc cttcgccaat cttccgtatg aggtgaaact gatccccgac ctttgcgccg
    16621 ggacctaact tggccacctg actactctat cacagagttt tggaatctag cttccctctt
    16681 cgtagtaggt gcgtacttgg ctgcgcacct tgtagggcct cgtcggaaac atcctcacct
    16741 gtctgttcgc tgttgcagcg cgtaagcagc tctttgccag gtcgaaacga cggcacccga
    16801 cgaggcggaa tcggcactgt cccgccggta tgcaggttgc gcccaagccg cgctttgctc
    16861 agcctgatgc ggaacgtccc aaagccgcgc acgtcgacca gttcaccgcg ctttagggcg
    16921 tgggccagct cggcaaacag atcttccacc agttcttggg tcttgtttcg cggcagcttg
    16981 tgcttgtcgg caagcaggct caccaaatca gattttttca ttgatgcact tcacaaaaag
    17041 tcgaccgcaa tcgatggcgg caccgtggtc gactacttgc ccggcaaggt cggcagcttc
    17101 ttgcccaccg cgtcggggat cagcagcgtc acatcaccgc tcgtcgtaag ctgacacgac
    17161 agccgcagct tggtgatgaa gtacgtattt cccagatgaa acttttccgc tttgctcacc
    17221 ggcggcagac tttcctcgcc gttttcgact ttgacccgac acaatccaca cgacccgcga
    17281 ccgacacacg ccgtcggtat caggatgccg gcgcgacggg ccgcgacaaa cgccgtttcg
    17341 tcgggtgcac agtctatcgt cttgttctgg ggcaaaaacg tgatgcgggg catgttggca
    17401 acggctcacg gaatcggtgg caaacgcggg acgactttag caaaacacgt cgccgggcgt
    17461 cgaggctact tcggatcgtc cagcccttca acgtagaagg gctccaggtc ttcagcggtg
    17521 agaatcagct tccgcagctg tttgtcgatg accggatttg acatgatggc ccaaaatgct
    17581 tcgcccggcc cgagtgtgtc gcgataacgc agaccgcttg gcacatcgag caagaacgtc
    17641 tgctgcgacg gtcgacccat cgtgctggct tgatcgagcc gaatctcaaa gcgcccattg
    17701 cccaggtctt tttcgacgat caagtggctg aggatcggca cattttcttc aatcggccag
    17761 cgtcccgtct gagccaccga gcgcagcgtc gacagcccaa ccaagtgcag cggattccac
    17821 ttcgctttgc tggcgatctt cggtggtgat ttgatccgcg acgcaaaacc cgaaccgacc
    17881 actttgtcgc gaaactccgg cggcagataa cttcccgatc cgtgtgaaga cacgctcgcg
    17941 aaacacagaa acacccagtc gccgacgctg ccggaaaatg gcttgccgtc gtgggtccgc
    18001 atttctttgg ccccaaatga aagcgcaccc gtctgggctc cgatttgata cgtctcgacg
    18061 gactcgtctt catcaaagcc cgtcttggcg ggaattttga cgattttttt ggcgatgatc
    18121 tttccgtact cggcgcgaaa cagctcgtac ccgtgcgctt tgcgacgatt tttcgggtcc
    18181 acggacatcg cgcactgatc cccgacgcga tagtcgagcg agcgcagata cagattccat
    18241 ttttcgcgcg ctttggtgct gctcgccgcg tctcgcgctg ccgatagttg tttggccgcg
    18301 gacagcgact cgaagcgttt ttttagttca ccaaaccgcg ccacgctctt ttcgaagtcg
    18361 tcgccgcgca cgatcttttc gatcaattga tcgcgctcag cgacgatggc cttcgcttca
    18421 tcgacaggct cgggggcggc agcaaccagc cacggaagaa acagtgcaag caaatggcgt
    18481 cgcaacggca acccccttta gtccaccggc atctggatcg catacgaatt tcgcagcgag
    18541 cgcccggcag acggttccag cttttccgct tcgtcgatgt ggcgctgcat ggccgcatag
    18601 tcacccagca tcatgtcaac catcaccacc gcttgcaggt gtgacaccgc cggcttgtcg
    18661 ctgactttga tgcaggcatc aaacagtctt tgcgcttctt tgaactggcg atgcgatgaa
    18721 tagaccgacg ccagcgtgca caagtcccag ccgatcagat tgggaaactt ttgcaaatat
    18781 tcggcaagct tctgcttgcc ctcttcggct tggcgcttgc tatcaatcgc ctgcgacacc
    18841 atctggcgca ccgattcgaa catcgcgctg ctcggcgcac gctgcatgaa cacttcgcca
    18901 tcgcgcagca ccgaatcgta gtcgcgcaag agcagatccg tcgacaccag catcaccgac
    18961 gctgaatcga actcacgctg tgaaaccggc cccggcggca agccttccaa atacagccgc
    19021 gcctcgcgaa ccagcgcatg atagcgacga gatccctgca aaagtcgcag aagctgatcg
    19081 gtcagcgcat ccagcttggc tcgctcggtc gtggtcgcca gatccttgcg cagctcggtg
    19141 atttttgcgt cggtgagccg cttttgctca gcttggtatt tttgaatccg ctcttccagc
    19201 gctgccaggt ctttgaccgc ataggaaaag ttctgatcaa tggccaccgc cgacgacaaa
    19261 agcaccagct tctgctgatc gttttccgcc gtcaccgctt gcccatacag atccagcgcc
    19321 tcgatcgtct ttaggtccgg acgcgcttcg gttccgtcga cgactttgcg cgccagttcc
    19381 ggcattccgg ctgatcgcag aagttccgcg gtgacacggt cttgcagacg gaaaaactgt
    19441 ttgacgctgc catcgacctt ggcactgccg atgatttcac tggtctcgac gcgaacaaag
    19501 cgtgcggtca agcgaatctg cgccgattcc tcttgatagg cgccaatgac catcaccgat
    19561 gcaccgacga cttttcccag cttggccgca gtgtcatcct cgacctcttt gagctgggcc
    19621 tgaacccgca tctccttgag cactttgtcg aggttgccac gctcgacgat tcgcaccccg
    19681 gcgaggctct tgaggtccga cgccacgacc tcgcgaatcg cttcgccgat gtgcggcttg
    19741 cctttgcccg tcagatcgcg aaatggcatg accgcgatcg tcggctgcgg cttcgctacc
    19801 accaccgatg gaaccagcac aagcccgaca agccacagca aaagaccgag accccagcgg
    19861 cccggcagca gttgacgcat agcaacacgg tatccaagcc gcgtattcat ctcaagcatc
    19921 cgacgaggaa tgtgcggtgt caggcaaaac tccggtgtaa agcggcgcgt cctgtggaat
    19981 cactttttcc cattttccga cgatgatcga cgccatgcag ttgcccagca cattgaccat
    20041 cgtgcgcgcc atgtccatca ccgcatcgac gcccaggatc gtcgcaatcc actccacttt
    20101 cagcccatac gccgcgcacg tcgaggccag caccacaagc gacgcccgcg gcaccgccgc
    20161 tacgcctttg gacgcgacga gcagcgtcaa catcatttca atctgcgtcc ccggcgacag
    20221 atactttccc gtcgcttgcg cgataaaaag cgacgccagc gccagataca aggtcgaccc
    20281 gtcgagattg aagctgtaac cagccggcag cacgaaccca acaatgtgcg atggcacgcc
    20341 cagtcgttcc atcgtcgcaa tcgctttggg tagcgccgat tcggaactgg tcgtcgaaaa
    20401 cgccagcaaa agcggctccc gcagctcgcg cagaatcagg cccatcttgg ccccggtaat
    20461 cagcttcacc gcgacgagca acagggcgac aaaacagatc agcgcaagat aaagcgacag
    20521 caccagcttg ccaagctgca ccatcacccc cggaccctgc gttccgacgg caaccaccat
    20581 cgctgcaaac accccaaatg gggcaaatcg catcacgtat tcggtgattt tgaacatcgc
    20641 atcggcgatg ccacgcagca catccagcac cggctttccg cgatcgccaa ttgccaccag
    20701 cgccatcgcg aacagcagcg aaaaaaacac gatctgcaag atcgcatttt gcgccatcgc
    20761 gtcgaggatg ctcgtcggga ccacctgttc ataaaagggc ttactcgccg gggtcggaaa
    20821 actgccggtg ccaccgatgg cgcccagccc tggcttgagg gtatttgcga tgccaagccc
    20881 gacgaccagc gccaccgccg tcgccagcca aaaccacagc agcgccttga cgagcaatcg
    20941 cccgatgtgt ttttcccccg cgccggccag cccgaccacc agcgtcgaaa aaatgatcgg
    21001 cgataccagc attttgataa gccgaatgaa cgtcttcgac atccactcaa acgacgctgc
    21061 tttcggcagc caagccggcg gatgaagccc gtcgcccaag tgcccgccaa gatacgcgcc
    21121 cagcgacgaa ccgagcacca gcgcaatcaa aatccacgcc gtcagaccga tttttttcat
    21181 cgcagcccca tctcccgcag gtagtcccgc gggcgcagaa agtcggaaag ccgcgcttcc
    21241 tcgctgccgg gctcgggacg aaagtcgtat tcccagcgca caagtggcgg caaagacatc
    21301 aaaatggact cgacccttcc gtcggtgcga aggccaaaca cggtgccgcg gtcatacagc
    21361 agattgaatt cgacatagcg accgcgccga tagagctgaa accgacgctg gcgttcacca
    21421 aaaggtgtgt cttttcgccg ctcgacaatg ggcagatagg cgggtaaaaa tgccgaccca
    21481 gcgtcgcgcc agaagctttg caccgtcggc aaatccgcct gcaaatagtc aaagaaaatg
    21541 cccccgatgc cacgggtttc ctgacggtgg cgcaagaaaa agtagtcgtc acaccaggcc
    21601 ttaaatcgag ggtagtagtc ggcatcgtgc ttgtcacagg ccagcgcgag ctgactgtga
    21661 aagtgcgctg cgtcctcgac aaacggataa tacggcgtca gatccgcacc gcccccaaac
    21721 cacgccgtcc gcccgcgtcg caaaaaacga aagttcgcgt gtgtcgtcgg cacatacgga
    21781 ttctgcggat gtagcacgag cgaaaccccc gtcgcgaaaa accgtgcgcc atcccctggc
    21841 agcgcgctct gcatttccgg tgacagctcg ccgtgcacca ccgacgtatt gactccggct
    21901 ttttcaaata ccgcgccgtc gtccaaaatt cccgtcagac cgccgccacc accgggtcgc
    21961 tgccaactgt cgaaacgaaa cgaagcgctg ccgtccaccc gcacaatcgc cgcgcagatg
    22021 tcgcgctgca gactgcggac ccactcgctg gcttcgtccg ccagaccatc gagatcacag
    22081 ccttgcgggg ccggtcggag cgattgggcc gccagttttt cgtttccgcc cagcgcaccc
    22141 gacgactttt gggtagtcat gtgttcattg tgccacactc gtcaggaaag ccgcgcagct
    22201 tccgttgacc ttgcttttgt ctttgtgcta gaagcgcccc gcaatgtcag cgcgaagagt
    22261 gattggcgtg atcggaggct ccggcctcta tgagctgtcg ggactcaccg gggtacacga
    22321 acaaaccgtc gagacaccgt tcggttcgcc gtcggatagc ttggtgattg gcgatctgga
    22381 tgatgtgcgg ctggtctttg tgcctcggca tggccgtggt caccgactga cgccgagcga
    22441 agttccctac gccgccaaca ttcatgcgct caagcagctt ggggcggagt atgtgctgtc
    22501 ggtgtcggcg gtcggttcgc tgcgcgagga aattcacccc ggccacgtcg tgctgcccga
    22561 tcagctgatc gaccggacca aagaccgccg cagtagtttt tttggtaacg gcgtcgtcgg
    22621 tcacatctcc tttgccgagc cgttttgtca tgatctgcgc aatgtgctgc tcggcgcgac
    22681 gcaggactgc ggcaccaaag tgcatccgcg cggcaccctg gtggtcatgg aaggaccggc
    22741 cttttcgacg agagctgagt cgcatatgta tcgctcgtgg ggcgctcaca tcatcggcat
    22801 gaccgcgctg cccgaagcca agctcgcgcg ggaagccgag ctgtgttacg ccacgctggc
    22861 gctgagcacc gactacgact gctggcgtga agaagaagaa gcggtcactg tcgatgcggt
    22921 ggtggccgtg ctcaaacgca atgtctcgct cgcgcgggaa gttgtccgtc gggcagccca
    22981 gcttatcgcc aagcttccgc cccgatcgtg cggttgtgcc cgtgcgacgg aatacgcggt
    23041 gatgacgcat gacatcgcgc cgcgtgacca tgagcgaatg aagctcatca tcggtcggca
    23101 catcccgccc ccgacgaagt aggatttggt tcgcgacgtt cactttccgt aacaaggagt
    23161 tcccttgagt tctcagcttg atctgctggt cgttggttcg gtggcgctcg ataccgtcga
    23221 gacccaggcg gcaacgcgca ccgaagtgct cggcggttcg gcgtcgtact tttcggcggc
    23281 ggcctcgttc cttacgtcgg tcggcctggt cggcgtcgta ggcgatgatt ttcccgacga
    23341 acatgcccag tttctgaccc gacttggcgt cgattcgcag ggtctggagc gtcgcgacgc
    23401 aggcaagacc ttccgctggc acggacgcta ttccgacgac tttaacaagc ggacctcgat
    23461 tgatacccag ctcaacgtgt ttgccgactt tgaaccgaag ctgcatccgg cgcacgcctc
    23521 ggcgcggctt ttgttcctgg gcaacatcga tccgacgctg cagcttcgcg tgctcgaaca
    23581 gatgaagagc gtcgagctgg tcggactcga cacgatgaac ttctggataa acggcagccg
    23641 cgaagccctg ctgaaagtcc tgcgccgggt cgacttcctg ctgctcaacg acgaagaagc
    23701 gcgcttgctt tcgggacggc acaacctggt ccacgccgcg caagcgattc agcgactcgg
    23761 acccaaaaat gtcgtcgtca agcgtggcga tgccggggcg ctgttgttcc acgacgagca
    23821 catctttgcc gcccctgccc tgcctctcac ggatgtcgtg gatccgacgg gcgccggaga
    23881 cagctttgcc ggcggcttta tgggcttcct gtcgcgaacc tggaaaggcg ggaacctcga
    23941 tggcatgctc gtccgtcgcg cgatgatctg cggcagcacc atggcaagct tctgctgcga
    24001 agacttcagc ctcgaccgct tccgatcgat cacccacccg atcatcgaca gccgcttccg
    24061 cgcgttccgg caactgaccc acttcgaaga cctcgtcctg taaatggctc gtacccaacc
    24121 gaagctgacc ggcgtgctgc cgcgacggta ttttggaacc gagtggcagc caccgtcgcg
    24181 tgtcgatgaa gaggccgcgc tggcgcttgc ccagcctgcc gcgattgttg aacttctgcg
    24241 tccgctcgtc gatgaaaagc gcgccgcacg aatcgacgaa gtgaccgcag gacggctgtc
    24301 gggtgtcatc gtggtgctcg aagacctgca tgatccccac aacggcggcg cggtcctgcg
    24361 gagcagcgaa gccatggggg tgtcggaagt ccacatcatt gcctcgcgtg agcgcttccg
    24421 cgtatcggac aaagtgaccc agggctgcga caaatggctc gacgtgatgg aacaccacag
    24481 cacggcgtcg tcgctttcgg cgctgcgcca gcgtggtttc cggctggcag cggcggttcc
    24541 tggggtggcg acgcagatcc atgatctgga tccactgcag ccgaccgcgc ttttgatggg
    24601 aaatgaacat gccggtctgt ctgccgaagc gcgcgcgctg tgcgatgtcg agtttgcgat
    24661 tccgctgcat ggtttttccg aaagcctgaa tttgtcggtg gccaccgcgc tatgtgtctt
    24721 ttcgctgacg tcgcgaaggc gagcggcgct gggtcgggct ggtgatctcg acgacgccgc
    24781 gctcaccgag ctgcgcgctc gcttctacct gcgggacgtc cgcggttccc tcgccatcgt
    24841 gcgaaatcgg ctcggggctc actagcccgc cgtcctgctc agctcgctgc tgcatccgtc
    24901 gccgggcctc gcgcttggca attcctccgc ggcgtcccag atccgccata tacgacctgt
    24961 cacgggcaat cgacaatccg ccttttcgac cggcttgcct cgctgtttca gcgttaaagc
    25021 gatgcgccag accattggcc tgcgcaacca ttcctccata gcttgcccat ttgcggagct
    25081 tgtcgcgctc catgttggca aagccgcgac gctcggtggg ttgatttttc gcggcctcca
    25141 gcggtgtcgg cttcgtgtct gcttgctccg gcggcacgtc ttcctccgca gccggggccc
    25201 cattgtgttc ggtttttctt ttcatggtca gaagtccttt cgacgttcct gctgaccatc
    25261 tgtcaatcaa acataagtcg cgttcgtcaa cccatattaa ccataataaa ccaataccaa
    25321 ttgtttatcg agagcaacgc agaaccaaat accaggcctt tttctatatc gaaattgtca
    25381 tatataagtg aattaccaat ctacccagtc cgttcaccgg ctcggtatcg gctgcgtggc
    25441 gtgcgatgga atgcccgctc gccgcgcgaa aacgtgccgg gccacttttt gcagcattca
    25501 agctggggaa caggcgcaga cgaatcgaat ctggacgtga tgcagggaag gacgttcgcg
    25561 acggcgggac aggacaaggc gaagcgccga gccgttggcc cagcgcccaa gcaaggacag
    25621 ggaaagacgg gaggacgggg acttacgcga cgggaccgga cttgtgttcg gctttgtcat
    25681 cgccagccgc atctgcgacg ggcgagctgg ccgacaccgc tgatgcttcc ggggattcgc
    25741 tcgcgtcgat cttggcggca gcgacagccg ggaccgattt ttctttggcg acgtagaacc
    25801 acaaaaccag accaatcagc gtgaccagca ccgcggcata ttgggccggg gtcagcccaa
    25861 acgcacgcgg atcagccccg attccgtcgc gaacccgcag acgatcgagc gcaaagcgaa
    25921 tcggtccgta cagcgtcgca atggacgcca caaacacacc caaccgcggc ttgaaaagac
    25981 cccagacaaa atagccaccg gcgagcgccg cagcgaacag cgcttcttca aaacccatgt
    26041 cgtggcgacg gaattcagcg ccgatgtgaa acagtccggt gcctctgggc gcgcagtgcg
    26101 cttgcgggca gggatagtcg acgcccagaa agtagagcgg atcgctcgtc agaagcagct
    26161 cgcccggatg atcgtgggcg acgtagcagc ccatgcgacc aaagaaccag cccgtcgcca
    26221 aaccatacgc cagtgaatcc gcgtaaggca gcacccgttc cccgttttgg cgacaccaca
    26281 aaaacagacc cagcacggcc ccggcaaagc cgaacatcga cgacaatccc gagcgaggat
    26341 cggcgagcgc tttgagcatg gccgtcgtcg tcgggtgttt ctgatacacg agcacgtcgg
    26401 caaagtgcga cagaagaaag ccggtcagaa gcgaccagaa gatcatgttt ttgaccttct
    26461 cttcgtcgag accgagaaac ttgccgcgtt gaatggtcag ccacgcacca accaagatgc
    26521 ccgtcgcgac gagcacaccg aaaggatgaa tcgccagcga gtcatcgatg atcggcagcg
    26581 agggcagttg cggataggga atcagcgccg aaagcatgtt caggcagcat aaaccaaggt
    26641 cttgtcgccg caagagccct tttcgcacga cactcggtct tcagacggcg gaaaacgtcc
    26701 gcgcccgcgc tgccgcgaca aaaagtcgaa tcagaatccg cacttcaagc gcaaatttac
    26761 gcaaaaaacc aaggtatgat gagggcgatg tgccctgccc gcttccttct tgccatgccc
    26821 tcgtgcagaa caagttctgc gcgggccagt ggaggcacat gaagcggtcg acggtggtgc
    26881 tgctcttgtt gtcgattgtg agcctgcttt tgaccgggct cgggctgtcg tatcggctgc
    26941 tgcgcttggg cctggaggcg gcgcaaactt cgacggaaca gcgtcttggg gcgattggca
    27001 tgacgctggc cgaagcgctc cggcggggat cggacacgac gctgcttggg ccggtggcgc
    27061 acgacagcca gcttgaggcc gcgtatctgc tcgatcagtc gctgcgtccc gtcgaagaca
    27121 agcacaccgt aagcctgctt cgcgtcgatc ctgatcgggc gctgcgagcg ctgcgcggcc
    27181 agccgcaagt cgggttggca tatcggctgg agctcgacga cgagccggac aattcagagc
    27241 ccaaacagag cccgcaaatt ttggcgggtt attttccggt gcagcgtcgc ggtgtcgtcc
    27301 agcttttggt gctggaagcc ggggagcgct ttaccaacct gcccgagcga ctgcggcggt
    27361 cggcgatggc gtcggcgggc gtggcagcca ttttgggcgc gctggcactt gtgctgacgc
    27421 tgcaagtgct gcgatcggcg gcgcggcagc agcgactcca ggccgaagct cagcgcggac
    27481 aggcgattcg gcagatggcc gcgtcggtgg ctcacgagct gcgcaatccg ctcgggacca
    27541 ttcgagccgg tgctgagctt ttgcgcgaac aggcggcaaa tgtcgaactt ctcgacgaca
    27601 tgttggcgga agtacagcga ctgagcgacc tgacaacgca ctttctgacg ttttcgcgcg
    27661 acgcaccgct gcaagtccaa gacgtggatg tggtcgcgct gtgcagtgaa gtttgttcgt
    27721 cgctgcgcat gcgttttccc catcctgagc tgtcgatcga agccccaccc gatggccaga
    27781 gcatttgcct aactgccgac ggtgcgaagc ttcggcaggt gctgctaaac ctgtgcctca
    27841 atgcgattca ggcgatgggc gaacgaggtc gactgcaagt gacagtccgc acgctgcgac
    27901 acgatgtggt cgaaattctg gtctgcgata gcggtcccgg cctgtcggac gaagcgtcgg
    27961 cgcacctgtt cgagccgttc tttacgacca agacgcaggg cactgggctc ggtcttttgg
    28021 tctgccgtca gatcattgag aaacacggcg gcaagctgcg ccttgtcccg cctggcgaac
    28081 gaccggctgg cgttcccacg ggtgcctgtt tcgcggttga catcccaatc cgttccgacg
    28141 cccgtgggac agaaaacgcg gcgctcgacg taagccacaa gtgatcatca tgagacacat
    28201 cctcctcgtt gacgacgaac cgaagcttgg aaaaatgctg gcgactgcgc ttcagtcagc
    28261 cggctacacg gtcgatcggg ccatgaccgg cggtgaagcg ctgaaaaaac tggcaagcga
    28321 gccgtttgat gtcgtgataa cggatctgaa actgccggat atgtcgggaa tgcaggtggt
    28381 gcagcgggtg cgcgaactga gcggagcccc cgacgtgatg atgatgacgg cctacgcaac
    28441 ggcagaaacc gcggtcgaag cgatgcgact cggggcggtc gactacctga tcaagccgtt
    28501 ttcgattgat gagctgcgga tcaaaatctc gcgactgatt gggcgacgga tgctgcagga
    28561 acaaaatggc aacctgcaag cacagctgga aaaagcgcag agcatcgaaa gtctggtcgg
    28621 tacgtcggct cggatgcgcg aagtgatgga acaagtgcag caggtgggcc ccaccgatgc
    28681 gacggtcctg ctcctggggg aaagcggaac gggcaagacg gcgctggcgc gggcgattca
    28741 tcagcagtca cgacggcgaa atgggccgct ttgtgaagtg cactgtgcgg cgctgccttc
    28801 gacgctgctg gaaagcgagc tgtttggtca cgagcgcggc gcgtttaccg gcgcggtgga
    28861 gcagcggctt gggcacatcg aaaaagccga tcgcggcacg ctgtttttgg atgaaattgg
    28921 cgaaattccg ccggctgtgc aggtcaagct gctgcgcttc attcaagacc ggaccttcca
    28981 tcgtgtcggt tcaagtcaag cgcgcagcgt cgatctgcga atcatctgtg catcgaatcg
    29041 caacttggcc caagcggtga aagacggcag cctgcgcgaa gatttttact accgcatcaa
    29101 cgtgttcccg attcacttgc cggcgctgcg cgagcggagc gatgacattc ccaagctggt
    29161 acaagacatc ttgcggcgac gtcatccatc ggtgacggtg agccagccag cgctcgatct
    29221 gctccagcgc tatccttggc cgggcaacgt gcgcgagctg gaaaatgtga tcgaacgtgc
    29281 ggcgatcttg gtcggcaccg atcggcccca tggtgaaaac gtgatcaccg ccgaacatct
    29341 gccgcgctcg tttgtcggtt ccgtgctgcc ggtgacgaca tcgggactgc ttcagccggg
    29401 attttcgatc gatcggctcg agcgcgactt gatctgggaa gcgctggcgc gcacgtcagg
    29461 caacaagacc gaagcggcga aactgctcgg gatcacgcga cgccggctct attcgcggct
    29521 caagagcatt gagaacgacg ccgaagcaga ggaaaatccg agcgacgccg atccaaacaa
    29581 aacggcgccc ggatcatcat gacagacacc aagaccccga cccctgttgt tccctacaaa
    29641 tcccatccat ggcacggaat ttccgtcgga gaccgtgccc ctgaaatcgt ctcatcgttc
    29701 atcgaaatgg ttcccaccga cacggtgaaa tacgaagtcg acaagcgatc gggacaccta
    29761 aaactcgatc gtccgcagaa atattcgtcg cagtgcccag ccccctacgg tttcattccg
    29821 cagacctatt gcggaaccca gtgtggcagt tacgccgggg acaaaacggg acgcagtgaa
    29881 atcgtcgggg acggcgatcc gctcgacatc tgcgtcctga gcgaacggac catcaatcac
    29941 ggcggcgtgc tggtgaacgc aagaccgatc ggcggatacc gaatgttcga cggcaacgaa
    30001 gccgatgaca aaatcctcgc ggtgctctgt gatgatccgg cctacggtgc atttcgcagc
    30061 atcgccgact gtccgcgcag cttgatcgaa cggctgcgcc actacttttt gacctacaaa
    30121 gacatgcccg gtcaaaacag tcgccgcgtc gagatcaccg acatctacga tgccgacgaa
    30181 gcccgccaca tcatcggact gtcccaagcc gactatcacg caaacttccg acccacttgg
    30241 tagtgcgcgg ctggcgctaa cacgccttcc ggaatgtcag gttgtagcgc gaattccccg
    30301 tcagtggatg atgggaatgt gcgatgggaa ggattccgtg aaagcgcagc cgtgaagcgc
    30361 cgccaaacac caccacatca ccatgcgaga gccgaacacg acgggtcgcg tctgtccgca
    30421 caaaaccacc caaaaggaat gtcgcgtcga cgccgatcga tacggaaaca atcggctggg
    30481 tgaagtcctg ttcgtctctg tcttggtgaa gtgaaagttt gttgcccggt tcgtagtgat
    30541 tgatcaaaca ggaatctgga acaaaccgat caaagcccgc tcgcgacgcc gcagactgcg
    30601 aaagcgaaca gagcgcgtcc ggaatcggtg gccagcttcg gcctgtctgt ggatcgcaag
    30661 cagaatagcg atatccactg cgcgatgaaa tccagcccac gctgccgcag cttgtcatcg
    30721 caaccgacat ccacagcccc ttgggggttt gcatcttttg cagcggtgcc aactgaatca
    30781 cctgcatcag cgcttgccac accgaatcca tcaccggcag cgcaaagtcc ggaaacagcc
    30841 acgcaccggg cagcagctct tccgcttttc gcggactttg gcgcagggaa tcgggtccaa
    30901 acaaaggcag tgtcatccgg tcatcacttc accacgtttt ccgcagggag ggaatgcgac
    30961 atcgcagccg ccagcgtcgc gcgaagctcc atcggaaccg ccgtcatccg caggtaataa
    31021 agacccggcg caagctcgcg cagcaccttg cagtacagat ccccggccag cagatcatct
    31081 gccgattgcg ggcgaaccat gcgcaaaagc aagttgccgt ttgtggccag cgtttcgcct
    31141 gtccgcagct ccattccgac atcagatagc gcttccacag ttccatgcgc agacagctcg
    31201 ccgacttgtt tttcgacgat gaaagcaaaa cggatcgaaa gcggaaccgg cagtctgtgc
    31261 agcgtttccg gcacgggctc tagcgctaca caataggggc ccgaaattcc caacacttca
    31321 tgcacgacga gcgttccctt cactcctttg ggcgtgagcg tctggctctg tcccagcaaa
    31381 acagagtcac cacagcgctg ccgtgtcact tcggaaatca acacttgccc accgacggta
    31441 taggactcta cccgtgatgt cagattgacc gtggttccca tcgcagtgta tttggcgcgc
    31501 agatccgatc cgatgttccc cagcaccaca tctccgctat gaaccccgat tcccatctcg
    31561 actttgggaa gaccgtgctc cacattccag tggttcactg cggccatcgc attttgcatc
    31621 tccagtgcac atgcgacggc gcgtcgttct tcgtcgccat gcgagagcgg cgccccaaag
    31681 attaccaaga tcgcatctcc gataaattca tcaattgtgc cgtggtagcg cgtgatgatt
    31741 tttgtcatca cttccagata gtggttgatc agcttgacca cttgttcggg cgacagctcg
    31801 gtgcacatgg tcgtaaagcc gcgcaagtcg gtcatcatca cggtgacaaa ccgccgctgt
    31861 cccccaagcc gcagcccatc gggagaatcc agaatctgct gcgccacttc atcggtcaga
    31921 tagcggccaa acgtctggcg cacgaactct ttggcagact ctgcacgcgc tctggcgcgc
    31981 gattccgaac gaaaaccaag cagcacacag atgccatagg aaaagcccag tgcggccagc
    32041 ggtgcaacca ccggaatgtg cagtccggaa tcgaagctga aaaacaccgt cagaagcaga
    32101 atcaagagtc ccagcgacag caccagaaag ccaagccccg gtgaaagcag cgccccaatc
    32161 agaccggcag agatcgcagc gatgaacgta agcacaatgc gcagaagcag tggtgcggtg
    32221 cgaaaaaaat catcccgcag cacgttggcg agcatcgtcg cgtggtattc gacacccgga
    32281 atcgccccca gcggactgct gtgaaaatcg gatgtggcaa gcgcggtcgc accaacgaag
    32341 acatgttttc cagacagacc gggatcgaca ccgccacgcc gccttacttt gccgattttg
    32401 gcgacaaagt gcatccgtga ttcatccacc acatcgaaca cagactcacg ctgggaatcg
    32461 ctgacaatcg acagatcgat catgtttccg gggtctgtga aatagtgcaa aagtccctca
    32521 atctgcgttc ctggtttcag ttttttcccg tcgggaaaat tgtcaggaat cagatattca
    32581 tgatcgtctc ccatcggatt gtctttcagg gtgacaagct gttccgatcg aagcagtgac
    32641 cacagcggaa cataacgaac cggcgaacca ccttctgcgc cttgccgacc gtggtagcga
    32701 atcagcgtcc ggcccagatc gtcgatggga atcttccgtt tgccacccaa atcgatctct
    32761 tcaccggggg tcaagcggat ctgtgacagc ggaacgccca gcgcatccgc cgccacacgc
    32821 aaagacaaag aaggaagaag ccgcttccca tgacgcagga tcaaaacggc atggcgaagc
    32881 acgtcatctc cgtcgagcat cacgttcgcc agcgcgacgc catgggtccg ggtggcaatc
    32941 ggatcaatga ccatgcgcgg catcacttcg cgacccatga gcagggaaaa ctctggcaaa
    33001 acggaagcag aaggattcca aggaacgacg tttcgatcga gcacttccat cgccgattcc
    33061 gtcggcaaaa gtcgcagttc acgcgaatgg gaagacagcg aataagtgaa gtagaccgaa
    33121 cctgcacgcg ccgctgtgtc cccaagcaaa gaatcccatt ctggatgatc gatgctggga
    33181 tcgagcaggg aaatatcaaa tgacagactg gccggatggg attcccagac tcgctctatc
    33241 agacgggaat actgatgtct tggcaacggc cagccaagcc gcgcaatcgt gtcatcatca
    33301 atcgcaatca ccaccacttc atccagcctg cccgcgctgt ctggttcatc ggtagacgtt
    33361 gcgacttcgg catgggtgcg ccgcggaaaa cgaagtctgt acgcgcgatc gacaaagctg
    33421 gcttcaagcc cctgttctat cggtgtggtt tcgatgatcg cattggcggc gattccgacc
    33481 agcagtccga cgatgatcgc gcggctccag cgggcccgaa ccgtagacag actggaaaac
    33541 aagcggtcgt cgatcgcatc gatcttggcg atgcagaccc gaactagttt gcggatcagg
    33601 tgccgcgttt gccacagtgc ggacaaaaga cagacgctgg caatcccggc agcgaccgct
    33661 gatccgagcg ccgatggaac ttcaaagcgg tgttcacacc actgtccaat tcggaatcga
    33721 ccgctgcgaa tcaggagtcc gattccgcaa agaaacagcg cgcgtccaag cgaccaaaca
    33781 gcgtctttgc gaagcatcac gggagcacgg aaaaggaata gaccggagag cttgccgatg
    33841 caaagccagc agcattcaca gccgagactc gccagaacca ttttccgacg ggagggcgcg
    33901 gtgacaagtg gagcctgggc tgcggtactt cggtctctga tgcttccacc aaaaagtccg
    33961 gatcgcgcgc cagttccacg cgataaaaca ccgcagaagg aaccgcagca aacgacaggg
    34021 attcatcttt gggaagcgtt cctacaagcg gagcaatcac gcgcggaatt tgcggcagtg
    34081 gcgttggttt ttcgaccaca ccagcctgat cgacacggct cccaaaaccg gcatcgacca
    34141 gcatcggatt tccgtgtgtt tctggaccca gcgcaacctg tccggtcagc gtttccagct
    34201 tggattttcc atctgtggtg ttgccaaaac gaaagctggt tccacgcacc gatgcgacgg
    34261 caattcggct gctggtgtca aaactggaac cggcggcggc tttttttaca gtggcttcga
    34321 tctgtccgcg ctgcacttcg atgcgaaccg agcgctctcc tgattcggca aagtcgagct
    34381 ttacaaaacg gacttgggat tccttcttga tgcgaatgcc actgccatca cgcagctcga
    34441 cttctgccac cccttcgggg ccggttcgca gcaaatcttc gacgaggatt ggcatcgacg
    34501 gttcagcagg acgcagcgcc gtttcggaaa agccgatgga aacagcgccg ctcagtttgg
    34561 caatccgcgc gaccacgctg tctggtgctc gttcggtgta atgaatggtc aagctggcat
    34621 tctgtccgct tggaacagac ggtgcgacgg caaagaccct gttttgtggg agtccggccc
    34681 gcaccagcaa gtcggcaact ttgcgcgcaa cagagagcgc ggttccatcc atccgaacag
    34741 catcatcggc gcgatacgag actgttacgg cgcgcatcaa gcgattccgc acaagcagtg
    34801 cagcgacgga agacagacag cgccggcctg cgtctttgcc aatgaaatcg gaattcaagg
    34861 gctggcccag cacgaccatg cccccctgga tcttgatgcc tccgcacggt tcgtccgcat
    34921 gtccggacag cgtgaaaccg aacaccagca acgacacagc caagccgatg aagcgatgcc
    34981 ggatcatggc attcccccag acagaatcca cagctcgaca cgacgattga ccgcgcgatt
    35041 ttttgcagag ctgttgggaa ccagcgcttt gtctgcacca tatcccaccg cgcgcagacg
    35101 tgtggctgcg attccgtttt tgatcaagtg ggtgcgcaca gcgtcggcgc gttgttgtga
    35161 aagcagcaga ttccgcttgg cgccacccac gttgtcggtg tgcccctgaa tttcaatcgc
    35221 aaggcgtggg tgatcgcgca aaagctgaac cagctctcgc aaaagggggc cgctgcgcgg
    35281 atcaagctgc gcgctgtttg gtcggaaaag gaactgctct ttcaaaatga gttggtttgt
    35341 cccgatgtcg atcttggctt gcgcccgttc cggacagcca tcgtcgtcga gccgaccatt
    35401 cacggtctcg gcttcaagcg gacagacatc ctgtgcatcg ggaattccgt cgtcatcatt
    35461 gtccagctcc ggacaaccat cttcgtcttc aaaaccatcg cgatcttcgg gaaccagtgg
    35521 acaaaggtct tcccgatcag gaatcccatc gccatctgaa tcgaggagcg cattgggaac
    35581 cagtgcatcg tttgcaggtg acagtttgga tgacgccgca gcgacggaag acggagcaga
    35641 ttccggagcc gtaacagagg ctgaagccgg ttccacaatc ggcggcgcca gcaatgcatc
    35701 cggacagcca tcgtcatcgc gataaccgtt tttgtcttcc ggttcatagg gacagcgatc
    35761 ttgggaatcc ggaatcccat cgtggtcctg atctctgtcg ccatggcgcg gtgccaaggc
    35821 caggcgcaga agcgtcgtaa gctgcggcga aacactggaa ccaacgccaa acagcgtggt
    35881 tccgattgcg gcgcctagct gaaacttctg ccacagcagc gtctgtcccg acaaaagcac
    35941 atcgagatgg acgatgtctt tgtcgctgac atcgtaaccg gtcacccgtg cgcgcagccg
    36001 cgtttccagc cccagataca gccgtccgcc cggcggggca aaacctaacg cgccacctat
    36061 ctgcacttcg ctgccagcaa ccacggtatg gcccggactc ccgatggcgg cgacgggtcg
    36121 atacagaaat ccgctgtcga cggtaaaacg gccactttcc gcaaaaatgc ccgcgatgac
    36181 aagccgcggc aaaagaatcc gaccgtcggc atcgccttca tgccgaggaa gtccatcgcg
    36241 acgctgtccc gcagcctgac ccattcccgg ccagccgtcg atcccggcat gcagcgaaac
    36301 tctatcgcgc tcgatgtcgc catacagtcg cgccgtcacg ccaaagccag gatcgccaag
    36361 cgccggtttt tcgatcggca cgtcgccact tgtcggatcg gcagtgccgc tttcgtagat
    36421 cgaaagcggt aggttcgcac ggatcaaaag ccagcgcagc ggcgacaccg cgacatcgag
    36481 cgaacccagc agcgaatgtg caacgagcgg accgtcgctg tcaggcagtc gcagcaagtt
    36541 gtggtcgtaa gcaaatgtca gcccgacggc aaagcgctgc gtcggtgcgt accaaggtct
    36601 ttcgacaaga aactgccagc tccccgcgct cgttggtgct ccgcgtgaaa gctgaaaacc
    36661 aagccgcgcc gaatctgcgt gtgcaggctg cggaaccgac aggcagatga ggccgcaaag
    36721 gcaccaccaa cgacgcagag gaaaacccaa acgcaccgac aagctcatgg caggtggcgc
    36781 gcatcttatg cttgtgtccg ctctgggcga aaaaaattga cgaacgatcc cagcatccac
    36841 ctgggcgtcg ggaaaatgcg cattttttag atttcatatt tgaaacgact cgacgaagcc
    36901 gcggcgctgg acggccagac cccatgcatc cgacgcacgc ggcccgaatg ttcaagtcat
    36961 tcgtggcaag gggtttcggc aaagtcgtct tcgtgctacg gtgcgacagg ttgcaatgag
    37021 cgaagcagcg cgcatcggtg agacaatcgg taagggccgg tttaagcttc agcgaagctt
    37081 gggtcgcggc accttcgggg acgtctacct ggcggagtct ctcgacccac aggcgacggc
    37141 aggccagcgt gaggccgtca tcaaagtcct gcacgcacag tgggccaaag ttccggaagt
    37201 cgtcgaacgt ttccgacgag aatctctggt cacgcaaaag attgaccatc cgaacgtggc
    37261 gcgggtgttt ttgcacggtg agctcgacga cggtgtgccg ttcattgcga tggaatattt
    37321 gccgggcaaa agtctgcgcg acaggcagga agatggcgcc atcccgtatg aggtggcgat
    37381 ggacatcctg atcagcatcg ccgacgcgct ctcggcagcc catcgcgctg gggtggtgca
    37441 tcgcgatctc aagccggaaa acattcagct gatttcgcgc aacggcaatc ccgactatcc
    37501 cgtcgtgctc gacttcggtg tcgccaaatt tctcgacgcg gcggaaaaac tgacgatgac
    37561 gggcgcgctg ctcgggacgc cggcgtatat gtcacccgag cagttccgcg gcgaaagcaa
    37621 cctgggtcca ccgtcggata tttatgcgct cggtgtgctt gggttcgagc tgttggcggg
    37681 acggctgccg tttgccggtc gcacgtttgc agagctggcc gtggcgcaca cgacgcagcc
    37741 ggcaccgcct ctgaccgggg ttccccagcc gctgaccgcg ctgtttgaac ggctgctgac
    37801 caaagaaccg cagcgacgac cgcgcgccga tctgctgatg caaagcctca agagcatcaa
    37861 agacggttcg ccgccgccga agctgcccga ggtctttgtt gatccctatg cagcgacggg
    37921 ccggctggat gaagccgtcc gcacggcggt gattagccaa gcggcgatgg agcgcaaact
    37981 ccaaaagcgt cagcagcttt acaccattct tctggccgcg gctgtcttcg tcttcacttg
    38041 tacgattgcc gtcgtcatct atatgttcct gctctaatcg actgagacac accccatgtt
    38101 cgacgatcgc cgactgagcc tgcttttgtt tcgagtgctc tgctacgtgg tgctgctcgt
    38161 catcgcgtat tcggtgctgt cccgtctgtc gctggtgctg acgccggttt ccgtcgcgct
    38221 ggtgctcgcg tttttgctaa atccgtcggt gtctgcgctc gaagaacgca aggtgccacg
    38281 ctggctggca gtcggtctga tgctgctgtt tctgctcggg gtgctgacgg cggtgggcgt
    38341 cacaattccg tacttggtgc gcagcgaaat cgagcggttt tctgcccagc tccccagcta
    38401 ccaagggctg attgatcgca aggtgctgcc gctgctcggc aaggtgttca aataccgcgc
    38461 caccagcatc ggcgactggc tcaagctgct caccaatcaa gtgacggagt gggtgcgcgg
    38521 tgcgatggga cagatttctg acgcgctcgg gacggcgatc ctgagcgtgg ccggtttgtt
    38581 caaatacctc gtcgcggggc tgctgattcc ggtgtttacg gtgtcgttcc tgatggacat
    38641 gccggcgatc ggggaaaacc tcaaagtcct ggttccgccg cggcacaaac agctcgtcgt
    38701 cgacatcatg gatgacatct atggagcgct cggaagctgg ctgcgtggac agcttaccgt
    38761 gatgctgatt caggcggcgc tgtactcgat tgggctgtcg attgcgggca ttccgctggc
    38821 gatcttcatc ggctgcaccg cgggtttgct ggcgttcgtt ccttatgtcg gcgtgatgat
    38881 cgggctttgc tcggcgctgc tcgtcgcgct gctcgaagtc tcgacggcag gtgcaacacc
    38941 gattctgggc gtgctggcga cctttggtgc agtccagctg ctcgatgcgc tggtgattac
    39001 gccacgcgcc gttggtggac gcattggcct gtcggcagtc ggcgtgatct ttgcgctgtc
    39061 gctgggcgga agtctgctcg gttatgcagg actgctactg gccgtcccgt ttgcggcggt
    39121 gttcaaggcc ttgtggccgc gactgaaaga cgcatacact ggcaccgcat tttaccgagg
    39181 cgaagccgta gcagatcccg tcgccgcaaa gacttcccaa agcgagtagc aggcccatga
    39241 ccaccaccga cagccccgaa accgatcttc ttgtcagcac ccaaggcgcc gtccgcatcc
    39301 ttgcgatgaa caggccgcag accaaaaatg gcctgacgct ggaacttttg tcggcgctgt
    39361 tagatgcgct gaaagccgcg gaaaacgacc ccgcggttcg cgccgtggtg ctgacgggtc
    39421 gtggtgatgc gttttcgtcg ggactcgact tccgcgcggc gatgcatttg gttcagtcgg
    39481 gcgggagcga ttttgccgag cagagcgaaa accgaattcg cagctacttc catgccgtga
    39541 ttcgtgcgct gacggcgctg cccaagccag tgattgccta tctcgacgga gtggcggctg
    39601 gttttggctg tgacttggcg ctcgcctgtg acctgcggat tggcacaaag aaagcgcgct
    39661 ttggtgaaat tttcataaaa cgaggcctga tgcccgacgg tggcggaacg tataccctgc
    39721 cccgcttgat cggcctgggt cgcgcattcg atctgatgtt cacaggtgac atcgtcgaag
    39781 ccgaagaagc ccagcgcatc ggcctgctga cccgaatttt cgacggagaa gcggaagccc
    39841 tcgcatttgc cgaacgcatc gctgctggcc ccccgctggt catggcggaa atcaaaaagt
    39901 cggtctacgc cgggcttggt cagtcgttca gcgacgcact cgaaaacgaa gtggccggcc
    39961 aggtcaaact gctccgctcg caagacttca tcgagggtgt cagcgccttc ctgatgaagc
    40021 gcccgccaaa cttcaccgga cagtaagcat caaagcgccg gcgtatccag acacgcaggc
    40081 cgctcgatcg acgggggcag cgcagacccc ggcaatatct ttcgcaagta cgcatagtcg
    40141 gtatcgcaga acaccgctgg ccccgacgga gaaagctctg ctcgttcgcg aaaactgcgc
    40201 aacgatccgg ggttcatgat ccgtccccgc aagctgccgt tgttgtgata cgaaccgttc
    40261 cggttgtcag gattcgcaag ccccaggctg tgcccgagct catgcgtcat cgtcgtgccg
    40321 atcaaactac ccagcacgaa aatcccgcac gccacctgat ggggacgatc atctttgctc
    40381 gccggacaca gactgcccga tgacagcgcc ggcaagagtg tcgcctcgcg cagcgtcgct
    40441 ggattcccac ccgtatcggg acgcagtgga tcgaaaacct gatcaaacag atcggcttcc
    40501 tgtggtcccg acttgatggc cgcaacgctg cccggatggt ttgaaaaacc gagaagctgc
    40561 tctgcaaaca cgccgccata acctggcgct ccgtcgttct gcgtcaccgc gttcacgccg
    40621 ccgattcggt catacagccg ccgattctgc atgtcgcgtc ccggcgtgtt gtcatacccc
    40681 aggtaaccga gcccattggg atcaggtccg ccaatgtcga cctgcgcgta gtaggcaaag
    40741 tcttcgggca gcggcatgtc ttcgccgcct tcgcgaaact cgatgttgat gccggcgtaa
    40801 tcacgtcgcg cgacttccaa aacccgcgca cgcaccgcat tgtcgacggc acgcagacca
    40861 aacaatcgca ggctgtcctg atacgacgac aaaaagcgca ccaccacgac ctgtttcgga
    40921 cgcaaaagcg caatccgcag tggcgtcggc ttgctcgcca ccgtttcttt gccgttgcgc
    40981 agcgtcatgg tgccagtgcc gacgaaaact cccgcggctt cacgcagtcc cccacgcgat
    41041 tccagggctt gcccaagcgc gtccgtttca tcgagcacgt atcgcccaac cggcgacgga
    41101 aaacgagcca gccacttgag cacaatttcg gtatccaccg gaatcatgac tggcgaattt
    41161 tcgagcgaga actgcccttg caaacgaacc agcgtcgctt gtcctggctg tccaccgata
    41221 aaaccagccc cctgcatttc cacgtactga cccaagctag cagcgctcgg cgctactccg
    41281 gtcagctcgg gtcgaacctg cgtcaccgca agcgtgcgag aggccgaaac cggcttcatc
    41341 gtcgttcccg gcggctgatt gcgcagcagg atcgtgccca caaacttccc cggcatcaca
    41401 ccgtgaatcg tcggagagta aggcaaaacg ccgttcgttc gatcaaagcg cgactgcggc
    41461 tgaaccggaa tctcgacctc gcgcacggtc tttccattgg cagaacatgg ctcactggca
    41521 ccttcgggaa gaaaacatcc cgacaaaagc gcaacggtca cgccctctcc gccgccaagc
    41581 agcagcccgt caccacgcac aatcagccag tcattgggat gaatctttcc cgtcgtgcgc
    41641 tgcggccccg ccagttccac cagatcgagc cgcggcgaaa gactcggcac aagtttgagc
    41701 gaaagctgta tcggctcggg cacatgttcc c
//
LOCUS       DAORGN010000016        40907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_335_length_40907_cov_6.305885, whole genome shotgun sequence.
ACCESSION   DAORGN010000016 DAORGN010000000
VERSION     DAORGN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 40907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40907
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_335_length_40907_cov_6.305885"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1303
                     /locus_tag="PKL17_03395"
     CDS             110..1303
                     /locus_tag="PKL17_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNK43800.1"
                     /translation="MFLAADPTPTPVVVSPELTPTAVSWLAKMQAPVDALSRGLDRWL
                     TDSMHASPAMVGLAHLLLTVALTILLAWFGGFVCRWLSRRLLSAGSNLASQLLALCAR
                     PLRYVVLFIGLSQAVEDAWPAMRGPGRWVAGTLFVVAIIVAVRGVSKLVRLLLDAVVG
                     PKVETYDAMQSLPVGARSSSGQALVPLLSRLAQVVLWLAGLIVILDHFGQNVSSVVAA
                     LGVTSLAIGLASQQALSNIIAGLVLALDHPFRIGDRIRLPGSEGGEVLDVGMRSTQIR
                     LADGSLLIVPNADLVAARLVNQSTERSVRGEVRLCVSALLNIDELSQHLEAAAAEVAG
                     SELVKPSPRVQLLTVADKVDLALVFWLQRQAELPVIEEQLRRVALRKVQDMVAKLQPP
                     STRPA"
     gene            complement(1311..1742)
                     /locus_tag="PKL17_03400"
     CDS             complement(1311..1742)
                     /locus_tag="PKL17_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43801.1"
                     /translation="MVNPAEATKFRIYAQRQSDGDVQDLYLLATDAGLAIWEAMQPPA
                     PIPAAALLGVFARYGKPIEEGIDVTPLLAREDAADAPLELALPSGVSVTLQRFRFMPF
                     GWVHPEDYLLWSVQGVEPLAAPAALVAHALSALARAYKPTA"
     gene            complement(1748..3196)
                     /locus_tag="PKL17_03405"
     CDS             complement(1748..3196)
                     /locus_tag="PKL17_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43802.1"
                     /translation="MRVPTVSRSAALFSVACSFLFACGEPDPQSKTASLLSAPDPSKT
                     VTIHVHGWNLSGASKTGTVGDDRGGGSTVDGIRRFTAMPHGSTSPTAQNQIIGTEYYG
                     KVFPSYYTATDVAEVSALKGIPRYATIVGKYARYVMQRSGAEGVNLTCHSMGCLISRY
                     LIENDVAGLASEGKIRRWVSFAGVVAGAKLADLDRGKWLDPLASLLGYDLIDVEHMSY
                     DWVQKNAAIYDGKRVDGNNPLFGNILVHHILSTNPKIDTALGIPLLDVLGSSAVPNDG
                     IVLDDEMYLHDQQPSARWTTPSGTQLLVSQSHHFAHHFDITEKVSAQALASAALVGSR
                     RVRVQLSKVSLRNDKEDPFFGKPPAEVVVESTVKYPYVNAIEPSAPTLDEVSMERRNA
                     KVLSMNKGETKTPNWMLFDGPVFDAQTSVTLNVKLSETDFYPAGGVNENILSPPAPLG
                     AFSGDVPLADGDYTVTSTDATFTLHVTVEKLY"
     gene            3313..4251
                     /locus_tag="PKL17_03410"
     CDS             3313..4251
                     /locus_tag="PKL17_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HNK43803.1"
                     /translation="MKPLVFGLAAVWALFGLHSAALALVLGLCLSLVLGNPFVVQTKR
                     WAPWILQAAVVGLGASMNLGVVLRVGLQGVGYTALGIAATIGLGIGLGKLLRVDRTTS
                     LLVSAGTAICGGSAIAAVASVIGASSADMAFSLMTVFFLNAAALFLFPQLGHALHLSQ
                     SQFGLWSALAIHDTSSVVGAAGQYGAQALEVAVIIKLTRTLWILPLTFAIGLLVTRKT
                     GGQSGPRKRPYFVLGFLLASALVTWIPALAPLGTKVTMLAKQAMVLSLFLIGANIDQR
                     DLSKLSLRPFLLGFLLWTAASAGTLLAIFRGLIRSE"
     gene            complement(4318..4716)
                     /locus_tag="PKL17_03415"
     CDS             complement(4318..4716)
                     /locus_tag="PKL17_03415"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNK43804.1"
                     /translation="MELRISLLGKKKISAHFDGYEVSTDQPKKSGGDGSAPSPFDLFL
                     ASIGTCAGFFAQSYCQAHNLPTDGIELVQTQVRDEKTRLVTSIKLELTLPASFPEQHR
                     DGVINAINACSVKKHFAHPPNIYVETKQRD"
     gene            complement(4808..5629)
                     /locus_tag="PKL17_03420"
     CDS             complement(4808..5629)
                     /locus_tag="PKL17_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43805.1"
                     /translation="MCKDPALGYLNDFGYNVVRLPRADIRPLDLIGRHRGSVERIGRL
                     DQLLTPSKNPLPTIRWNCPVSGLEGQLSNQLDFSFAAKIVASFVQGLGGNPAVSSKLS
                     QLSKLQIQLTDVKSDRTEPAAIGQLLDGATLETSNPLWAPFLRGDGKLFVIFETLKSS
                     RLKLHVDEAHSASASVDLPILREVAGANVAVEVKKESGMSLAFSGTQQLVFGFKCLEL
                     SLSDGFIELRLAPPSVRAAMGVRSPDALRGEVPQQSSDQDGAALLCEDDLIDLPV"
     gene            5732..7864
                     /locus_tag="PKL17_03425"
     CDS             5732..7864
                     /locus_tag="PKL17_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNK43806.1"
                     /translation="MELTLGAGVGQGFLADLRFRPKPDADLQDLCVDRPVPLDLEALA
                     EKNHATEYGKLLTQQLFSDTALREAWLRAAATVEATAIRLRIRLRMKPSPGSEPLHAI
                     KWESLYDPQIQFRLTQSERISFSRSFPTDSLAPLESLPEKRRKVLAVLCDPVDHEQFA
                     LPSLDTAKHGERIVAAASAFPCEVLRRTSDNDGVSLRRLMERLRSGIGILYLVCHGRL
                     VKLRSDQPPKSMLYFEREDGLTNPVEGDLFVEQLKGLASPPLLVVLGSCHSAGDSDRL
                     VLDSLAPKLAAAGVGAVIGLQGEASLDLLEKLLGDFFTELASDGCVDRALSVARVRRA
                     EQGDWWRPVLYMRLRGGLLFASEKAFSSEPANSNNAAEFPGADSAPKGEKTLDAVLSH
                     PLCLAELPVLKERFVENYRVVFLLDWYKELHDRLQELEKSYKSLISQLPVSDDRDFAG
                     AVFAETQIPIEELQHGLGEVLEHLKSDPEAKFAYTVRNLLAAESQTSEAVQGIDVKKL
                     SRALGSIFRELRRAIPQSNNYLQTQVQQLRLEKLRESISNVLRLAVQIGVSSAVQRQL
                     QSLLDLLSQRHANLHAQSEEHHHWQRVDNDLQRILELVRAHGEAAESEKDEISEQWGE
                     LAEPMHIITSKLTDEKLRGKLLSLHANLSSYVAADQMVEFRRGFLTFYSLCGHCFVKV
                     DKALRELCKSLSGDFRTIDAVLFLHLIG"
     gene            7869..>10988
                     /locus_tag="PKL17_03430"
     CDS             7869..>10988
                     /locus_tag="PKL17_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNK43807.1"
                     /translation="MESKKILRFDSLGDIRKEQTRIQHLRRAARGQVVAEEPGILPLS
                     KEDALPALLREEIIAFVEQTEASGALLAEEAERWTAQGILDYWTDVLERAGVSRTAAA
                     LDPFDPAELPEIPDDKCPFVGLDAFGESQASRFFGRSAAIQELAKFVENHRLITVVGS
                     SGSGKSSLVSAGLIPLLRAAAERGTTPWQILPTMVPGESPDVALRALLDESLSDKDPA
                     SSAERLLALGKGKRLLLFVDQFEELFTVCESEEKRRAFAAELVRLSTSPSAQHTVILT
                     LRSDFEQQIASLPELHALYPAGRFALGVMMEDELREAIERPARAVNLRFEDGLVKQIL
                     RDVSTESAALPLLQFTLLQLWKRRRGNLVLRESYEALHGAAGAVREVADQVYRDLKLP
                     EKQEVARRIFLRLTYPKEGRYEVFRQRLRRSEIIRDERPDYADDVIRDFVAARLLRRV
                     PGQTPDSEQIEVAHEALVRNWKTLLDWIDLSKQDVVTRRRLDFMVEEWIRLGEAEAGC
                     LDAVQLNEAKVWVSSDRAKLHGYHKRLPDLIAKSAALQDEASQKETKQRRRTQRIVLS
                     LSAVVVAAAVMVAWSYKRRTDLEKQASSNAEKEAKLAKDLTAARQKSVQAMRMIGEDP
                     LKALALIGEASQLPHYESRTLLYRLLGHPLPTAKLAGHSAAINALEVSPLGTLLLSAA
                     EDKRVRLWNLRGQTLAQLSCHTQGVNGARFHPSFGKNPEILTYGDDGKVLLWKPRNPP
                     RASAAMGLPSGEMSEAVNLPKDGFSTEQVNLAKSAGDSATKLDCSAQEVTVGTSAVVQ
                     EAISKSKDDSATQRILWADWSPSAEFIAYRSELGKLGADTGALSFVRAAGGQPEVLAA
                     TNVARAFFLPGDGATLVWQDTEDGVHLCNLTSCQPVKLGLPGDFRNLYLDRLSGRMGV
                     VAFANSVRNPDKGGPTRNPKQTPLPLEPARIWAWDRNGQPRLSVDGFRHEDLLTISDI
                     SWSPDGSTLLIVGRESRDPGVEKLSLWRIENGALGKIPWAPSTVTMPLLAPPPPPQAR
                     AGSQTPQRSN"
     assembly_gap    10989..11088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11089..12741
                     /locus_tag="PKL17_03435"
     CDS             <11089..12741
                     /locus_tag="PKL17_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNK43808.1"
                     /translation="AQFSPDGRYIVACDKERRVLLYERSAEGPSYRVSAVLGPFVRQW
                     TRCFFGGDGKRILLGYSGEPGLHVFDTAGHFVTDIADGFPRVLVRTDYYVTQRVSSER
                     LLWRFARPPIPMLSSAQDNVNFAVTDRNETKLVTASGNTLFLWNDKGEQVGRTRSLAD
                     QVMSLRISDDGQRVLAMTSSDRMRCWLWDLTTDSDPVAIDTGDESVYCFMFHHEGKTR
                     IGGLSGRGEVWQWSDSGVRLPVTLDLKLAAAQVPSVRVTISYPRAGTYLVVHTDGATR
                     KSLLFDWNGTLIRELPRQGDSYLLDAADHGDFFVTLSGQTVLRFDSRGNPLPLAIPNG
                     MRVADKPLLTVSDTGQYIALSGSLSESPGVWLWDTRAGGPPRHLPTTSGVRKILIHET
                     SRALAMETEQKRVLIWDLSKDIDRPVRRASGRLIAVAAEVANRPWLFMQERDGSLLMW
                     HVRDGVHHRFATLREDWSIDDVLVSPSGRFLVPLSTVRFSIDSSRRVRTSHVWPLDES
                     RLSTQMDMVVKSRSLRDADDGASQDQEDEEQEKDELLNQLGD"
     gene            12766..14529
                     /locus_tag="PKL17_03440"
     CDS             12766..14529
                     /locus_tag="PKL17_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNK43809.1"
                     /translation="MSESLLYFNGVNALTGDYARAPASVQGWSAQTLSVPSTTLLADL
                     EKRKEQTEQSDGKLAAARKHYAELQADLATLLRMGSADVNQLQSLRRQIADAERFLVR
                     NTHAGVRQGIDPLSLSQAGWGIIFPARIAADRQSQILEALLPLLSRRKMQAGDRFRTY
                     VGAGGYRTGDTKARFLSRHGAAPAGPASPEAVPYYLLLIGSPEEIPFEFQYHLDIQYA
                     VGRLHFLDIAGYHNYAKSVVQTEEKGSPKAQKLTLCGVQNPSDPATEKTERFLTKPLS
                     DEFGQTAHGWQIEKLGAEQTSQKGILAQLGGSQTASLLFVACHGIEYPTGHPLQRARQ
                     GALLGNRWCRSENRPLSEDDFVAGHHLSSSADVSGMMLFAFACYSAGAPLPAEYPSPD
                     GIVPATAADVPFVGSLPMELLSHPRGGALAVIGHVERAWGFAYGVDDARQRSHTAVFH
                     SALSRLLLGHPVGHAMEFFNEHYAERATALSELVFQILNHRTYDPQILGELWCESADA
                     RGYVVLGDPAVRFVYPSPSLAEAVPQVAEAVPQVTDIRDEQTAFPCPQGISPADWERT
                     PRAVKEWAARLQKQSAETPVR"
     gene            complement(14543..15211)
                     /locus_tag="PKL17_03445"
     CDS             complement(14543..15211)
                     /locus_tag="PKL17_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="expansin EXLX1 family cellulose-binding protein"
                     /protein_id="HNK43810.1"
                     /translation="MGCGTVTSNSSTNSDIPQLPGSQPQIGSTQTGDGTYYGATGAGA
                     CSYDPSPSNLMVAAMNAPQWAASAVCGTCVDVTGPKGTVTVRIVDLCPECKSGDLDLS
                     EQAFTMIADKSAGRVKISWTPVACAVSGPLGIHFKEGSNAWWMAVQVRNSRLPIKDIE
                     LQQSGNWVKLQPQTFNYYVADTPDPGPGPYTFRITAQSGQQVVESGIALQVGQTVSGT
                     QQFQ"
     assembly_gap    15259..15358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15398..16174)
                     /locus_tag="PKL17_03450"
     CDS             complement(15398..16174)
                     /locus_tag="PKL17_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43811.1"
                     /translation="MDKNTLLSSALVLLSVGSSLAAPPAPTAKPAAKPAPKPAAPKPA
                     EMPKGCSPMESGFVMQPTEVALKCDETWDKCESDIPLRAKNCTGEFQSVYRMEMYEKG
                     RRSLELEFDPAPIVPNGSVWKEAIPWTSPGELEVVIYFRAPGSTSEQSVRSAIHVANK
                     ALAQAKEACEKCSGVWGRYGVNHNQGCNCKTTDAGKECHDGDECQGLCLFRRYDNEAR
                     EEGVCSETQRMSGCHAVVMKGQSQLKPRIPPPKKLPTCLD"
     gene            complement(16229..18505)
                     /locus_tag="PKL17_03455"
     CDS             complement(16229..18505)
                     /locus_tag="PKL17_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43812.1"
                     /translation="MLHTPPLPSQPYVSVREHLQSELDRLTLLLRLHKLADTDDAQPE
                     KAELIDMLTTLSLQIRERLSLSDASGQTLPMMRLAQSLSLSDAEVRLVTLLLLRETEE
                     AAQQILAPFFGPHPQGTVAHYLTLLRQQGLPEPQIQEALRPGSTLRRHGFLLLADDAA
                     WAPTTPLLFQRLKLSDRLAAFLRGEDRPKLHTLPHGVSLYFAQRTLAQLTLPAGLLER
                     VRDALHESAQPLLGDPDGPVLPPLCLTGLRRSGRKALLAALLPGRPLVVLSATSLLGE
                     RASLLPTVQAALGEAALQQGLLYIASAELLGELDPSLFEQLRRLLLGSRVRVVLAADG
                     PIDALWTRLPDLLRIDFPMPDRATQVALWNSLLPVDMGRERGFDVRVLASHYSLPAGA
                     IQRSASEIVNGARIDGGRRPQLTMAAAREAIRKQLGNRFGDLAQLVTTSLSWEDLVLP
                     AEILDRVLEVVAYARYREQLLGKWGFERLLPYGRSISVLLAGAPGTGKTMAAALVAKE
                     IGLELFRVNVARVVERFAGDTEKNLARIFDEAKRQQVALLFDEADTLFGKRLDVRSSQ
                     DRYANMAVSFLLQAVESHDGMLILTTNNEKLLDEAFKRRLRFRIALPLPSEYERAKMW
                     QAMLPKDTPKERGVDFAALASEFDLSGGAIKNAVVRAALRALAQGGEVSQELLRQCAK
                     LECEEMGILVTSDAGLISDADDDHDEPLNDHDADDESDDDDHDDDLATPTVGIAVGNQ
                     DDQETARAVRGKPSDETS"
     gene            18630..19769
                     /locus_tag="PKL17_03460"
     CDS             18630..19769
                     /locus_tag="PKL17_03460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HNK43813.1"
                     /translation="MAQETTPKTKPRRATIVSPSTAAKAEDAAAPGAEPELVEGGAAE
                     SAEPGEDDADIESLVGAAVDAMPDEDIVLAADALAPEILSDGKRGGSLVKSDPLTVYM
                     HEIRRYPLLDRDEEHRLAVEYSKSRDPRIGRRLITANLRLVVKIAHEYKRAYRNLLDL
                     VQEGNLGLLQAVEKYDPHRGVKLSSYAAWWIRAYMLKFILNNWRLVKIGTTQAQRKLF
                     FNLSKERDKLEADGFVPTSKLLAERLHVPEQEVTDMSLRLGQSETSLDAPVGRDDEGG
                     RRQVDLLESPGNTRPDLTAESEEFRALLRSKLSEFEQTLSGREKLLFQERLMADQPLT
                     LQDVGERFGISRERARQLEARLLGRLKAFLKKELGDAVQVAMGLE"
     gene            complement(19783..20460)
                     /locus_tag="PKL17_03465"
     CDS             complement(19783..20460)
                     /locus_tag="PKL17_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43814.1"
                     /translation="MGNEDRPKLSWREIDKRRAGGTTSTGRGAAPSERPHHEEATRQK
                     QYRAALEAAFAKGELGKLADKLNLTGRSTPAEEPKPVAPASPPPAAAPAAAASDGKAN
                     PAEAAAPAEGGKKTAAKKKPTEDKASLLRKLAEAVNRQEISRAAEKYLARFPIPDDHE
                     FLEQLLEHEKESRIREAMTRIGELLDKRHLPKRTRALIGKLRYLTETNPIDDIRELAK
                     TLLSRLT"
     gene            complement(20489..21505)
                     /locus_tag="PKL17_03470"
     CDS             complement(20489..21505)
                     /locus_tag="PKL17_03470"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNK43815.1"
                     /translation="MDIVVVGAGSWGTALACVLCENQHKVTLWGRDAAQMSEAETTRE
                     NRRYLPGLRLPDSLRFTADLHAAIATLPKDAPSLIVAVVPTHTMRQVFADIAAEIPPQ
                     AIIVTASKGIENDTLLTMDDVLSQVLPSHLHDRIAVLSGPSFAKETIEKYPTAVVAAA
                     KQRPIAEAVQRAFQVGYFRVYTSDDVIGAEVGGAVKNVIAIACGMADGLGFGHNTRAA
                     AITRGLAEITRLGVRLGCNPLTLAGLAGMGDLVLTCNGPQSRNRQAGLLFAQGKTLVQ
                     VQAEMKQVAEGIRTVRSVHDLAAKVGVEMPISAAMYRVLYEGKPVGQVMSDLLGRPPR
                     HELG"
     gene            21890..22741
                     /locus_tag="PKL17_03475"
     CDS             21890..22741
                     /locus_tag="PKL17_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK43816.1"
                     /translation="MSRKLLHSLVLCSVVMAPLAALADEAAAQYQKGLQFMQLGKLDD
                     AQASLEEALKLRPGYAAAELTLGNVLRKKKQCDKAIPHYEAAIKLQPDDHFAHGNLGF
                     CLATAGKDADGIRMLAKATELAPNEVQWQSSLGALYRRAKQFDKALPHLEKAVQLDPN
                     NPDALRNYAVALRQAKNLDKAAEVTRQAIRLLPNDSNLRFDLGVIYRVQGKTTEAIAA
                     YREAIKLEDKNAEAWYDLGVLLSQDRQKQEAIEAFNKYIDLVGSKNPTQIEGVRQQIR
                     GLGGTAH"
     gene            complement(22738..23958)
                     /gene="ftsA"
                     /locus_tag="PKL17_03480"
     CDS             complement(22738..23958)
                     /gene="ftsA"
                     /locus_tag="PKL17_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNK43817.1"
                     /translation="MAKREEIIVGLDIGTTKVACLVGEITEDGIDIIGVGTQPCAGLK
                     KGVVVNIDSTVAAVAKAVEEAEHMAGVEITQVYAGIAGSHIKAFHSEGVVAIRDREVR
                     PGDIERVIEAAKAVSIPQDREILHVVPREFSIDQQDGIKEPLGMAGVRLEAKVHIVTA
                     ASASAQNIIKCCTRCGLEVQDLVLEPYATAESVLHSDEKELGVALLDIGGGTSDLVVF
                     SEGALVHTIVLPIGGHQLTNDIAVGLRTPMSEAERLKHKHGCALSSLIAEDETIEVPS
                     VGGRPPRVMPRTVLSDILQPRVDEIFSLVRDELERCGLSDMLASGMVVTGGSTLLQGL
                     PELAEEILGMPVRRGVPQGIGGLSDVVKTPIYATAVGLVLYGARKQDGLQLSQSSERR
                     GVWRRMRSWFAEVF"
     gene            complement(24013..26463)
                     /locus_tag="PKL17_03485"
     CDS             complement(24013..26463)
                     /locus_tag="PKL17_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNK43818.1"
                     /translation="MRDVHVQEKTVADLSWTALLAKLAERCHTTRGMQLAKELPLCGS
                     IAQAQTRQREVSEARALQDGGEALPFGGITDVRAFVERSEKAGVLVPDELTEIGKTLT
                     AGQRLRRHILSRSARLPTLTQHAEPIAELPEVAGPISDSFDDHGALRDSASPALRGLR
                     QRVQGLLSDLSRRTDSLLCEGHIEPYLQDRFVTQREERYVVPVRADARTKIRGIVHGV
                     SASGATVFVEPEEIIELNNRLKLAQLEVAEEERRILAELSRLVEESSPRILRNLEILA
                     YLDLMDGAAKLASDLRASPVELVDASEQTEKPAQIDLRNGRHPLMVLSGVQVVANDVL
                     LDAGAALVISGPNAGGKTVALKLTGLCVLLSRAGLHVPAQEGSRLPWFDRVLTDVGDD
                     QSLEKNLSTFSAHVLNLCEFLNRADRGTLILLDEIAVGTDPEQGAALAQAVLEGLAAR
                     GATVLVTTHYDRLKAMAAESTKFKNASVGFDLKALKPTYKLHLGIPGPSSALPVAERL
                     GLPLPLIRRAESLLKSGQSSVERLMQTLADERERTETMRTEAERLRRIAASEAAEARR
                     AHDDARVELQKARRRAYDEAIETLQSARRELSDLKAALKATRHKTEDGQSDGAQGELS
                     QASKRLDELSQSVRGLVPEKAGPVGRVPSAEELTQGMRVYVTRLSASGVVASDVSRGK
                     VVVQVGALRLTADVAELLILGGSSDRPAPSPKAHRHPPQVVMATSAPIIPPRTPDATI
                     DLRGERLPVAVARTEKFVDDALREGRPAVFILHGHGTGILRSALREHFVAFPGVRKLY
                     PAEPGDGGDGVTVVELDT"
     gene            complement(26520..27014)
                     /locus_tag="PKL17_03490"
     CDS             complement(26520..27014)
                     /locus_tag="PKL17_03490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNK43819.1"
                     /translation="MARPKVYFDITIADQPAGRIVFELFNDVVPKTAENFRALCVGHH
                     AGHYQGSIFHRVIPSFMLQGGDFTNHNGTGGRSIYGHKFEDENFQLKHDKAGLLSMAN
                     AGKNTNGSQFFITTVLTPWLDGKHVVFGQVVEGMDLVRKIEACGTDSGRTTKKIAIAK
                     SGEL"
     gene            complement(27135..27599)
                     /locus_tag="PKL17_03495"
     CDS             complement(27135..27599)
                     /locus_tag="PKL17_03495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNK43820.1"
                     /translation="MQKNATPRSLRRDYRHFLSIPTRFLDNDVYGHVNNVVYYSFFDT
                     VINEFLIAQGGLDIHHGAVIGLAVETQCRFFAPLSFPQIIDAGLRIGHLGRSSVRYEI
                     GLFAQTTDKDSEPAAAEGHFVHVFVDRETRTSTPIPDSIRTALARLAVPSAD"
     gene            complement(27641..28960)
                     /locus_tag="PKL17_03500"
     CDS             complement(27641..28960)
                     /locus_tag="PKL17_03500"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HNK43821.1"
                     /translation="MSCAHYFAQAQGGDGCFAVDASNIVFGRGALLEAGELAKSLGLR
                     RVALFTDPVVRKLPPVQLVTQSLRAAGIDSVCYDEVRIEPSQASFEQASQFYAEGRFD
                     GAISVGGGSVMDTAKAALTYATHKAPFLRYVNRPIGEGVALPGPLPPHIACPTTSGTG
                     SECTGIAVCSVPLFGTEGRHCKTGLAHRALRPRLALIDPVCTETLPAHVVAATGFDVL
                     CHAIESYTALPFSRRARPESPLARPMSQGANPFSDVGSLEALRLCGTYLLRAIRDAED
                     REARDQMMYAAALAGIAFGNAGVHVPHAMAYAIGGLAPTLTLSGYPQTRPLVPHGLAV
                     VLSAPAAVFHLAATSPGRHLRCAQALGLDCRDIADADAGRFLSGELRRLLGESGLPSE
                     LSQAGYCLTDVPALCEGAALQTRLLGNAPRHVGPGDLEVMFRDSFAS"
     gene            complement(29126..29554)
                     /locus_tag="PKL17_03505"
     CDS             complement(29126..29554)
                     /locus_tag="PKL17_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43822.1"
                     /translation="MGRALLGLIKGVLVGGGIGFALLKLGSPAGILAYLFCALLGATV
                     GVVCGKAPWKAATLWTPILKMVFGAAIGVGLYAVGHRFMPALSLSIDGVANKLSIQSA
                     TVLAPIIGALYGLFVELDDGGADSPSAPAKKSLPAKDLDI"
     gene            complement(29697..32084)
                     /gene="priA"
                     /locus_tag="PKL17_03510"
     CDS             complement(29697..32084)
                     /gene="priA"
                     /locus_tag="PKL17_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNK43823.1"
                     /translation="MSEAEPVQAAQQTQPSLVEEPSPRLCLVAVMSPAPGPYLYGVPD
                     DMTAMPLPGDRVLVPLGGQPRVEGIVLESIEGAAATQTLSVSGFARHALRNLHMRLSG
                     AKLPADLLGMARFVADYYLSPLGEALRLLLPPSEHAQLIERVKLTDEGLRLSHKLSGV
                     LLSPEVADLDPREIELLQALGSRRSPQAFVSVDTALSHERLSKRPKADWRSRLGELHK
                     RHLVEIHCDVKVGLSKEPKAKVDEHTLPSAPQGLVLTDEQAHALQVLLLALSAAEHAK
                     PRRSGYQGFLLHGVTGSGKTEVYLQLIGEARKRGQTALVLVPEIALTPQLSARFSSRF
                     PGQVAVLHSALTQAERASAWRKILRGEVSIALGPRSAVFAPLSHLGVVIVDEEHDSSF
                     KQQDGVHYHGRDVALWRAQQAGAIAVLGSATPSLEALALSRSGKLTRLTLHKRATGIA
                     MPKVEVIDLKQHVKATESALLTGPLLEALRTTVEAGEQAILLLNRRGYAAFLLCQSCG
                     HRIECPNCAVTLTMHKGRQSLICHYCDHREPMPKDCPLCQQKSLVPLGLGTEKLLEQL
                     QLRFPEARIERLDRDTAGSLTRVLSAMHRHELDILIGTQMLAKGHDFPGVTLVGVVLA
                     DTGMGLPDFRAAERTYQLLSQVAGRAGRASRPGRVLIQTYNPEHPAVSCAAQHDYLSF
                     AEAELEARLALSYPPSVRLGLLRIDGEDPYKVAQVAKELHDILQAELSAQPDGSSLLG
                     PAEAPLSKLKGRCRWQLMVRSPSVRSLHRLLRLTLLHKVPAGLRVHADVDPGSTL"
     gene            complement(32081..33025)
                     /locus_tag="PKL17_03515"
     CDS             complement(32081..33025)
                     /locus_tag="PKL17_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK43824.1"
                     /translation="MNDVPPVIPLEQRRPRALPEDFTHRTSRGVYHGPLDAKRGGPAS
                     PDQKTVEAPDEPAALVSRQSLLQVLWVLVRTEFRARYRAQALGIVWSLLNPLVMMGII
                     SVIFTQMFRSAEKFFPIFLLIGLLMWQWFSTSLNSATGVFVHNADIIKRTVFARPLLP
                     IASVLSYGINFSIECLVLIAFIPIFPGAFKLSPALLLVPVLLLLTISLMAGIVLATSV
                     LNVIYRDIAYLVSTGLLLLYWATPVFYPIDVIPFPFRTILQANPLAGLMTAMRMAIMR
                     GEFPTPLGWAGIVIPTVLALLIGWRVFRHYETMVLDYV"
     gene            33109..34419
                     /locus_tag="PKL17_03520"
     CDS             33109..34419
                     /locus_tag="PKL17_03520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNK43825.1"
                     /translation="MRALRTFVPIASALLLAGCPSEKPKQAASTSKPATPAASASGPN
                     QGNQSMAPEPGDLPAPSDVAAAPADAEKSASGLASKVITKGTGTERPEAQDTVKVHYT
                     GWMTNGKMFDSSVKRGSPTEFPLKGVIPGWTEGVQLMVVGEKRRFWIPGKLAYGDSPT
                     PGRPYGTLVFDVELLEIKRGPKPIPAPEDVKAVPADAQKTPSGLASKVLTKGKGTLHP
                     TADDTVEVHYTGWMTTGEMFDSSVVRGQPAKFPLGRVIKGWTEGVQLMVQGEKRRFWI
                     PADLAYGDTPRRPGGPHGMLVFDVELLGIAQAPKVPPDVKEPPKDAKKTPSGLAYKVI
                     AKGTGQKHPTATSTVEVHYSGWTTDGKMFDSSVVRGEPTSFPLNGVIPGWTEGVQLMV
                     EGEKARFWIPGKLAYGDTPTRPGAPAGMLVFDIELRKILDTPAH"
     gene            complement(34436..35230)
                     /locus_tag="PKL17_03525"
     CDS             complement(34436..35230)
                     /locus_tag="PKL17_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43826.1"
                     /translation="MSNKRFFGSLLAVSLCLATGFVACGGAAANDDDGGEASAAMSQS
                     GAVEYMLTSPINSADAATAASALAAAQWWPAGCATRQKDPTQPIVTITLNNCSGPFGL
                     LKWNGTLTATFAKNPDGTLNVKAASTNMTVNGNPATFTMDRNITVSGTGTTRDVKGST
                     TWTRTNANGVQVTHSNQWTATVDSATKCRTVNGTGATTAGTVSIATTTKDYKVCRPNG
                     VDGCPSGMLTHVATPSNATVMVMFDGTNTATVTGPNGTRSVSLICQ"
     gene            complement(35399..35803)
                     /locus_tag="PKL17_03530"
     CDS             complement(35399..35803)
                     /locus_tag="PKL17_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNK43827.1"
                     /translation="MSEAVRDLLIFDGDCKFCRAQAQRLVRMAGPQIEPHPLQEEGLL
                     DRLGITYDEAMAAMQLVTTLGRFSGLQAVVVALRHRPILGWLAKLYYVPGLRQLGDLG
                     YRLVARYRYAIWGRAVARGECEGGSCAIHLRH"
     gene            complement(35833..36486)
                     /gene="ddpX"
                     /locus_tag="PKL17_03535"
     CDS             complement(35833..36486)
                     /gene="ddpX"
                     /locus_tag="PKL17_03535"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: protein motif:HMM:NF007557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HNK43828.1"
                     /translation="MRRRWLGLCLFGVGCATGQAATAKTAPPLVDVMQVAPSVVVDLR
                     YATTQNFTGQVLYPKSARCYLRADVAQALALVASDLGKAGLRLKLWDCYRPHRVQYQL
                     WDIVHDERYVANPHKGSRHNRGAAVDLTLVDAQGQELVMPTPFDDFGEKAHRSYQQLP
                     PLAIQNRTTLEQAMAKAGFVGMPTEWWHFDFARWQGYPLDDIPFDVLAEPPKKSAQP"
     gene            complement(36502..37602)
                     /locus_tag="PKL17_03540"
     CDS             complement(36502..37602)
                     /locus_tag="PKL17_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNK43829.1"
                     /translation="MSSADSPVPHFEEPRYDPFRFMKWGALLLLLLTLLSTGVYWLLG
                     KHYGRADWTPMNCLFMVVITLTTIGYGDWLELKNLHLAEGFTMFLALVGIAVPAFLVS
                     NVTALVVEGLFTDVFRRRRMQKKIAALRDHIIICGVGSTGLHCVTELMSTGRQIVAID
                     HNETKLRHLNEELGEFPYIVGSAESDEILMMAGIERAAGLIACLTEDKDNVFVTLTAR
                     QMNRNLRIISKVLEDNSRRKLAIAGANGTVNPTHIGGLRLVSELVRPTVVTFLDNMMR
                     DRRSTHRFEQLQVGEKSAVAGKRLQDASLRGKTSALVVAIKPPESDQFIYNPTADVVL
                     SPGTQIVLLAGTNDMATLRTLITGHAEGPHEL"
     gene            38091..38870
                     /locus_tag="PKL17_03545"
     CDS             38091..38870
                     /locus_tag="PKL17_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43830.1"
                     /translation="MNPDLDFDPSQYTRLPPYLDNATTVALGRQLVAAASGIVAPAVK
                     KSTSVVSRATQKLADGMAELVGLDAPQQKRPIDQAADHSWAGLQLRLLGWLELPAADH
                     PEVAEAQALHAKLFPNGLRFTQLEYGAQWAEAEARLSWLKGAGQLGVLERLCGKPFVA
                     ELLRAHAAYAEMVGTDAKKRATLASRPDLTQLRKVAQKAMLAHQIQLVALLMSGEAVQ
                     QAEKALRPLDDYREKLMPQKPAKKEDPSPAPETPDPAPAGA"
     gene            complement(38992..39414)
                     /locus_tag="PKL17_03550"
     CDS             complement(38992..39414)
                     /locus_tag="PKL17_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNK43831.1"
                     /translation="MPAWQSVGAQRYYVDGDLFVMELSGLVTLDEITALLLPQEALFA
                     HYERVLTLCIARDVQLPTADVRRFLAARGKRVDVTRLYVVVVTNNTLLRTIIRLIERA
                     TALITSSPLHNTFVSSEAEAWQWVENQRREHPPKRPPT"
     gene            complement(39547..39939)
                     /gene="atpC"
                     /locus_tag="PKL17_03555"
     CDS             complement(39547..39939)
                     /gene="atpC"
                     /locus_tag="PKL17_03555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNK43832.1"
                     /translation="MLSLHVVTPEGKTLTEEVDEVSLPGLLGEFGVLPGHVPMLSAVK
                     AGTVRYRKGQDRGVLAVGPGFAEVSGQNKVVALVQRALPQAKLSAEAAEKLLADANEK
                     LKGEKAERGAAEADRDFALAQLEALGKK"
     gene            complement(39943..>40907)
                     /gene="atpD"
                     /locus_tag="PKL17_03560"
     CDS             complement(39943..>40907)
                     /gene="atpD"
                     /locus_tag="PKL17_03560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNK43833.1"
                     /translation="VGKTVLLMELIHNVAKAKGGFSVFAGVGERTREGNDLYREFIEG
                     GIIVENDLQKSKVALIYGQMNEPPGARARVALTGLTVAEYFRDVEGRDVLFFVDNIFR
                     FTQAGSEVSALLGRIPSAVGYQPTLSTEMGALQERITSTDKGSITSVQAIYVPADDLT
                     DPAPATAFAHLDATTVLSRAISELGIYPAVDPLDSTSTLLQPHVVGEDHYRCARDVQR
                     VLQRYKELQDIIAILGMDELSEEDKQTVARARKIQKFLSQPFHVAEQFTNIPGKLVPL
                     ADTIRSFRGIVDGEYDHLSEQAFYMVGSIEEAVEKNKKLLAEAK"
BASE COUNT         7839 a        12182 c        12487 g         8199 t
ORIGIN      
        1 tatccgttcg agaagttcga ctcgatgatc aaagaggcgc tcgccgccaa gaagtaaggc
       61 gcctgccaag acggcttctg cgccgtctcc gcgtataatg accgccgtga tgttccttgc
      121 ggcggaccct accccgacac ccgttgttgt ttcccccgag cttacgccca ccgcggtcag
      181 ctggctggcc aagatgcagg cgccggttga tgcgctgagt cgtgggctcg accgctggct
      241 gaccgattcg atgcatgcga gcccggcgat ggtggggctt gcgcacctgc tgctgacggt
      301 cgcgctgacg attctgttgg cttggtttgg cggctttgtc tgtcgctggc tgagccgacg
      361 gttgctttcg gcgggaagca acttggcgtc tcagcttctg gcgctgtgcg cgcggcctct
      421 gcgctatgtg gtgctgttca tcggtctgtc gcaggcggtc gaagatgcgt ggccggcgat
      481 gcgaggtccg gggcgctggg tcgcaggcac gctgtttgtg gttgcgatca tcgtcgcggt
      541 gcgcggggtg tccaagcttg ttcggctgct tctggatgcg gtggtcggtc cgaaagtcga
      601 aacgtatgac gcgatgcaga gcctgccggt cggggcgcgg agtagctcgg gacaagcgct
      661 ggtgccgctt ctgtcgcgac tggcgcaggt ggtgctatgg ctggctggac tgattgtgat
      721 cctggaccac tttggccaga acgtgtcgtc ggtggtggcc gcgcttggtg tgacctcttt
      781 ggcgattggt ctggcgtccc agcaagcgct gtccaacatc atcgctgggc tggtgctggc
      841 gctggatcat ccgtttcgga ttggcgaccg cattcggctg cccggcagtg aaggcggcga
      901 ggtgctcgat gtcgggatgc gctcgacgca gattcggctt gcagatggca gtctgttgat
      961 tgttcccaat gcggacttgg tggcagcgcg gctggtcaac cagtcgaccg agcgttcggt
     1021 gcgcggggag gtgcggctct gcgtctcggc actgctcaac atcgacgagc tgtcgcagca
     1081 tttggaagcg gcggcagcgg aagttgcagg cagtgagctg gtgaagccgt cgccgcgggt
     1141 gcagctcttg acggtggcgg acaaggtcga tctggcactg gttttctggc tacagcgcca
     1201 agcagagctg ccggtcatcg aggaacagct ccgccgggtg gcgctgcgca aagtgcaaga
     1261 catggtggcg aagcttcagc caccctcgac aagacctgcg tagtttttgg ttacgcagtg
     1321 ggtttgtacg cgcgggccag cgccgaaagt gcgtgcgcga caagtgccgc cggggccgcg
     1381 agcggttcta cgccctgcac gctccacagc aagtagtctt ccggatgcac ccagccaaac
     1441 ggcatgaagc ggaagcgctg cagcgtcacc gacacgcccg acggcagcgc aagttcaagc
     1501 ggcgcgtcgg cggcatcttc gcgggccaaa agcggcgtca catcgatgcc ttcttcgatc
     1561 ggcttgccat agcgcgcaaa cacaccgagc agcgccgctg cgggaatcgg cgcaggcggc
     1621 tgcatggctt cccaaatcgc aagtccagcg tcggtcgcga gcagatacag atcctggaca
     1681 tccccatcgg actgccgctg cgcgtagatg cgaaacttgg tcgcttctgc cggattcacc
     1741 atgccttcta atacagcttt tcgaccgtga cgtggagggt gaacgtggcg tctgtgctgg
     1801 tcacggtgta gtcgccatcg gcgagcggca catcgccact gaatgccccg agcggagccg
     1861 gcggcgacag aatgttttcg tttacgccgc ccgcgggata gaagtcggtt tcggacagct
     1921 tcacattcag cgtcaccgat gtctgggcat cgaacaccgg cccatcgaac agcatccagt
     1981 ttggcgtctt ggtttcgcct ttgttcatcg acaggacttt ggcattgcgg cgttccatcg
     2041 agacttcatc gagcgtcggt gccgatggct cgatcgcatt tacatagggg tacttcacgg
     2101 tcgattccac caccacttct gccggtggct tgccaaagaa ggggtcttcc ttgtcattcc
     2161 ggagcgacac cttgctaagc tggacccgca cccgccgcga tccgaccagc gccgccgacg
     2221 ccagcgcttg cgccgacacc ttttcggtga tgtcaaagtg atgcgcaaag tgatggctct
     2281 gggacaccag aagctgggtt ccgcttggcg tcgtccaccg cgctgaaggc tgctgatcgt
     2341 gcagatacat ttcgtcatca agcacgatgc catcgtttgg aaccgcggaa gaccccagca
     2401 catccagcag cggaatcccg agtgcggtgt cgattttcgg attggtcgac agaatgtggt
     2461 gcacaaggat gttgccaaac agcgggttgt ttccgtcgac tcgtttgccg tcgtaaattg
     2521 ccgcgttttt ctgcacccag tcgtagctca tgtgttcgac atcgatcagg tcgtagccca
     2581 aaagactcgc gagcggatcg agccacttgc cgcgatcgag atccgcaagc ttggcgccag
     2641 caacgacacc cgcgaacgaa acccagcggc gaattttgcc ctcgctggcc aggccagcaa
     2701 catcgttttc gataagatac cgtgaaatca gacagcccat cgagtgacag gtgaggttca
     2761 ctccttcggc tcccgatcgc tgcatcacgt accgggcata cttgcccacg atcgtcgcgt
     2821 agcgcgggat tcctttgagc gcagagactt ccgcgacatc ggttgcggtg taataggaag
     2881 ggaagacctt gccgtagtat tcggtgccga tgatctggtt ctgtgccgtc ggagatgtgc
     2941 ttccatgtgg cattgccgta aaccgacgaa tgccatcgac tgtggaaccc ccgccgcgat
     3001 catcgcccac cgttcctgtc ttgctggccc cagacagatt ccagccgtga acgtgaatcg
     3061 tcaccgtctt gcttggatcg ggcgcgctga gcagagacgc cgttttgctc tgcggatcgg
     3121 gctcgccgca agcaaaaagg aatgaacaag cgacggaaaa cagagcagcg gaacgggaca
     3181 ccgtgggcac gcgcatcgca acctccagat cctcaaaagg ggaagtctgg ggcgtatcac
     3241 attcgttttc ccaaacacaa tcgccgcttg gctgacaatc ggcaaaagag gtgatacaca
     3301 gcctgggaat tcatgaaacc tctcgtcttt ggactggctg cggtctgggc tctgtttggt
     3361 ctgcactcgg ctgcgctggc gcttgtgctc gggctctgtc tgtctctggt tttgggaaat
     3421 ccgtttgtgg tgcagaccaa gcgctgggcg ccctggattt tgcaagcggc ggtggtgggt
     3481 ctgggcgcga gcatgaacct gggcgtggtg ctgcgcgtcg gtctacaagg ggtcgggtac
     3541 accgcgctcg gaattgccgc tacgatcgga cttgggattg gactgggaaa actgctgcgt
     3601 gttgatcgaa ccacatccct tttggtcagc gcaggaaccg cgatttgtgg tggcagtgcg
     3661 attgctgcgg tggcgtctgt gatcggggcc tcatccgcag acatggcgtt ttccttgatg
     3721 acggtgtttt tcctcaatgc tgctgcgctg tttctgtttc cgcagcttgg acacgcgctt
     3781 cacttgtcgc aaagtcagtt tggtctgtgg agcgcgctgg cgattcatga cacaagctct
     3841 gtcgtcggtg cagccggtca gtacggcgcg caagcgctgg aagttgcggt gatcatcaag
     3901 ctgacgcgga cgctgtggat tttgccgctg acgtttgcaa tcggacttct tgtcacgcgg
     3961 aaaactggag gacagagcgg gccgcgcaaa cgaccctact ttgtgctggg attccttttg
     4021 gcgtccgcgt tggtgacgtg gattccggcc ctggcaccgc ttgggacgaa agtgacgatg
     4081 ctggccaaac aagcgatggt gctgagcttg tttttgatcg gcgcaaacat cgatcagcgt
     4141 gatcttagca agctgagtct acgaccgttt ctgctcggat tccttttgtg gacggcggcc
     4201 agcgcaggga ctctgctcgc gattttccgg gggctgattc gcagcgaata ggaattcccg
     4261 aaatcgctgc ggaattcctc tgctcgcgct gcccaaacag cgtcacgttt gtcggcgtta
     4321 gtcgcgctgc ttggtctcta catagatgtt cggcggatgc gcaaagtgct ttttcaccga
     4381 gcatgcattg atggcgttta tcactccatc gcggtgctgt tccggaaacg atgccggaag
     4441 cgtcagctcc agcttgatgc tggtgacaag ccgcgttttt tcatcccgca cttgcgtctg
     4501 cacaagctcg attccgtcgg tgggcaggtt gtgggcttgg cagtagctct gcgcaaaaaa
     4561 accggcgcag gttccgatcg atgccaaaaa cagatcgaac ggcgacggcg cgctgccatc
     4621 tccaccgctc ttttttggct gatcggtgct gacttcatac ccatcgaagt gggcggatat
     4681 tttctttttc ccaagcagag agatgcgaag ttccatggtg gctatctgca gctaagatgc
     4741 catccctttg ccgcggtgcg tcaaaaaaat cgagccacca acagagaccg gcgcaaacgt
     4801 cgagggatca gaccggcagg tcgatcaggt cgtcttcaca gagcagtgcg gccccgtctt
     4861 gatcgctgct ttgctggggg acttcgccgc gcagtgcgtc cggagatcgg actcccatcg
     4921 cagcacggac acttgggggc gcaagtcgta gttcgataaa accgtcgctg agcgagagtt
     4981 ccagacactt gaagccaaag acaagctgct gggttcccga aaaagccagg ctcatcccgg
     5041 attccttttt cacttcaacc gcgacatttg cacccgcgac ttcgcgcaga atcgggagat
     5101 caacagacgc cgatgcactg tgggcttcat cgacatggag cttgagccgg ctggacttca
     5161 gggtttcaaa aatcacaaac agcttgccat ccccgcgcaa aaagggagcc cacagaggat
     5221 tgctggtttc aagcgtcgca ccgtcgagca gttggccaat ggcggcaggt tctgtccgat
     5281 cgctttttac atcggtcagt tggatctgaa gcttggaaag ctgcgacagc ttgctactga
     5341 cagccggatt cccacccagt ccctgcacga acgacgcgac aattttggcg gcaaaagaga
     5401 aatcgagctg gttgctaagc tgtccttcca gaccggatac gggacagttc cagcggatgg
     5461 taggaagcgg atttttcgac ggagtaagga gctgatccag tctgccgatg cgctcgacac
     5521 tgcctctgtg cctgccgatc aaatcaagcg gtcttatgtc agcgcggggc agccgcacga
     5581 cgttgtaacc gaaatcattt aggtagccga gcgcgggatc tttgcacatg ggttcggatt
     5641 ccttctgaca cagcaagata tcacagcttc gattgacttg acttgcgcgc ttgcgtggcg
     5701 agatagtcta catgtgctct cattcggcga gttggaacta acgcttggtg ctggcgtagg
     5761 gcagggattc ctggccgatc tccgctttcg tccaaagccg gatgcggatc tacaagacct
     5821 ctgcgtagat cgtcctgttc cgctcgatct ggaagcgctg gccgaaaaaa atcacgcgac
     5881 agaatatggg aagctgctga cccagcagct cttttccgat accgcgctgc gtgaagcctg
     5941 gcttcgagct gcagcgacgg tagaagccac agcaattcgg ctccgaatcc ggctccgaat
     6001 gaagcccagt cccggatcgg aaccgcttca cgcgatcaag tgggaatctc tgtacgatcc
     6061 acagatccag tttcggctga cccaaagtga gcggatttcg ttttcgcgtt catttcccac
     6121 cgatagtctg gcaccgctgg agagtcttcc ggaaaagcgg aggaaagttc tggccgtgct
     6181 gtgtgacccg gttgatcacg agcagtttgc gctgccatcg ctcgatacgg caaaacacgg
     6241 tgagcggatt gtggcggcgg catccgcttt cccttgcgag gtcttgcgcc gcacttcaga
     6301 caatgacgga gtgagtctgc gccgcctgat ggagcgactt cgcagcggca taggaattct
     6361 gtatcttgtc tgtcacggtc gcctggtcaa gctgcgcagc gaccagccgc ccaagtcgat
     6421 gctctacttt gagcgggaag acggactgac caatccggtg gaaggtgatc tctttgtcga
     6481 gcagttgaaa gggctcgcca gtccgccact ccttgtcgtg ctgggttcgt gtcatagcgc
     6541 gggtgattcc gatcgattgg tcctagactc gctggctccc aagctggccg ccgcaggtgt
     6601 cggtgcggtg attggtttgc aaggggaagc gtctctggat ctgctggaaa aactgctcgg
     6661 ggattttttt acagaactgg caagtgatgg ctgtgttgat cgggcgcttt ccgttgcgcg
     6721 tgtgcgacgg gctgaacagg gcgactggtg gcgtccggtg ctttacatgc ggctgcgcgg
     6781 cggacttctg ttcgccagcg aaaaagcgtt cagcagtgag cctgcaaaca gcaacaatgc
     6841 agcggaattt ccgggcgcag acagcgcacc caaaggcgaa aagacgctcg atgcggtctt
     6901 gtcccatccg ctctgtctgg ccgagcttcc cgtcttgaaa gaacgctttg tcgaaaacta
     6961 tcgcgtcgtg tttctgctgg attggtacaa ggaactgcac gacaggctgc aagagctgga
     7021 aaaaagttac aagtcgttga tcagtcaact accggtgagt gacgatcgtg actttgctgg
     7081 tgcggtgttt gcggaaaccc agatcccgat cgaggaactt cagcacggac ttggcgaagt
     7141 gttagagcac ctgaagtctg atccggaagc aaagtttgcg tatacggtgc gcaacctgct
     7201 tgcagcggag tcacagacca gcgaagccgt gcagggaatc gacgtgaaga agctgtcgcg
     7261 ggcgctggga tcgatcttcc gcgagctgcg ccgggcgatt cctcaatcga acaattactt
     7321 gcagacacag gtgcagcagc tccgcttgga aaagctgcga gagtcgattt caaacgtact
     7381 ccgcttggcg gttcagatcg gcgtaagttc ggctgtgcag cgtcagcttc agagtctgtt
     7441 ggacttgctt tcgcagcgcc atgcgaacct gcatgcgcag agcgaagaac accaccactg
     7501 gcagcgggtg gacaatgatc tgcaacggat tctcgaactg gtacgcgccc atggtgaggc
     7561 cgccgaatcg gaaaaagacg aaatcagtga acagtgggga gagcttgccg agccgatgca
     7621 catcatcacg tccaagctga cggatgaaaa gctgcgcggc aagctgttgt ctctgcatgc
     7681 gaacttgagc agctatgttg cggcggatca gatggtcgag tttcggcgcg gattcctgac
     7741 tttttacagt ctgtgtggtc actgtttcgt caaagttgac aaagcccttc gcgagctctg
     7801 caagtcgctc agcggagatt tccgaaccat tgatgccgtg ctgttccttc acctgattgg
     7861 gtaacgtcat ggaaagcaaa aaaatcctgc gcttcgactc cttgggtgac atccgcaagg
     7921 aacagactcg gatccagcat ctgcgacggg ctgcgcgcgg tcaggtggtc gcagaggaac
     7981 cgggtatttt gccgctttcc aaagaggatg cactgcccgc cttgctgcga gaggagatca
     8041 tcgcatttgt tgaacagaca gaggcatcgg gagcgcttct ggccgaagaa gccgagcgct
     8101 ggactgcgca gggaatcctc gattactgga cggatgtcct agagcgtgct ggcgtttcac
     8161 gaacggcggc agcgctcgat ccgtttgatc cagcggagct gccggaaata cccgatgaca
     8221 agtgtccgtt cgttggcctt gatgcgtttg gggaatcgca agcgagccgg ttttttggac
     8281 gcagcgcagc gattcaggag ctggccaagt ttgtcgaaaa tcaccgcctg atcacagtgg
     8341 ttggttcttc cggtagcggc aagtcttccc tggtcagcgc tggtctgatt ccgcttttgc
     8401 gggccgcggc ggaacgggga acaacgccgt ggcagattct gcccaccatg gttccggggg
     8461 aatcgcccga cgtggcgctg cgggctctgc ttgatgaatc gctttcggac aaagatccgg
     8521 catcatctgc ggaaagactg ttggcgctgg gaaaaggcaa gcgactgctg ctttttgtcg
     8581 atcagttcga ggagctcttt acggtctgtg aatcggagga aaagcggcgc gcttttgccg
     8641 cggagcttgt gcgtctttcg acttctcctt ccgcgcaaca tacggtgatc ttgacgttgc
     8701 gcagtgattt cgagcagcag attgcgtcac ttcccgagct gcacgcgctg tatcctgcgg
     8761 gtcgttttgc gctgggcgtg atgatggaag acgagctgcg cgaagcgatt gaaagacccg
     8821 cgcgggctgt gaatctgcgc tttgaggatg gtttggtaaa gcagattctg cgcgatgtat
     8881 cgacagagag cgcggcactt cctctgcttc agtttacctt gctgcagctt tggaaacgac
     8941 gacgtggcaa cttggtgctg cgcgagtcct atgaagcgct gcatggggcc gccggagccg
     9001 tccgcgaagt cgcagatcag gtgtatcgtg atctgaagct tccggaaaaa caagaggtcg
     9061 cgcggcggat ctttctgcga cttacctatc ccaaagaagg tcgctacgaa gtctttcgcc
     9121 agcgattgcg gcgttccgaa atcatccgtg atgagcggcc agactatgcc gatgatgtga
     9181 tccgtgactt tgtggcggcg cgacttctgc gacgggttcc ggggcagact ccggattccg
     9241 aacagattga agtggcgcac gaagcgctgg tgcgcaactg gaagacgctg ctcgattgga
     9301 tcgacttgtc caagcaagat gtggtgacac gccggcgact cgacttcatg gtcgaagagt
     9361 ggattcgctt gggtgaagcg gaagcgggct gtctggatgc ggtccagctt aacgaagcca
     9421 aagtctgggt gtcatcggat cgggccaagc tacacggcta tcacaaacga cttcccgatc
     9481 tgattgcaaa gagtgctgcg ttacaggatg aagccagcca aaaggaaacc aaacagcgtc
     9541 ggagaacaca gcgaattgtc ctttcgttaa gtgcggtggt tgtggccgcg gcagtgatgg
     9601 ttgcgtggag ctacaagcga aggaccgatc tggaaaaaca agcaagcagc aatgcggaaa
     9661 aggaagccaa gctcgccaaa gacctgaccg cagcgcgaca gaagtcggtc caagcgatgc
     9721 gcatgatagg agaagatccg ctcaaagcgc tggcgctgat tggggaagcc agtcagctgc
     9781 cccactatga atcgcgcacg ctgctttatc gcttgctcgg gcacccactt ccgacggcaa
     9841 aactggccgg acacagtgct gcgatcaatg cgctggaagt ctctcctttg ggcacgctgc
     9901 tcctgtctgc tgcggaagac aaaagggtgc gcctctggaa tctgcgcggc caaacgcttg
     9961 cgcagctgtc ctgtcacacg caaggcgtaa acggggcccg gttccatcca agctttggca
    10021 aaaatccaga gattttgacc tatggcgatg atggcaaagt gctgctgtgg aaaccgcgga
    10081 atcccccgcg cgcgtctgct gcgatgggtc ttccgtcagg cgagatgtct gaagcggtga
    10141 atcttcccaa agatggattt tccaccgaac aggtaaacct tgcgaagtcg gctggggact
    10201 ccgcgaccaa gctggattgt tcggcccaag aagtgacggt cggcaccagc gctgtggttc
    10261 aggaagccat cagcaagtcg aaagacgatt ccgccacaca gcggattttg tgggcagact
    10321 ggagtccgag cgcagagttt attgcgtatc gatccgagct gggaaagctg ggcgctgata
    10381 ccggagcact ttcatttgtg cgggccgcgg gcggtcagcc ggaagtcctg gctgcgacca
    10441 atgtggcgcg ggcttttttt cttccaggtg acggtgcgac gctggtttgg caggacaccg
    10501 aagacggcgt tcatctgtgc aatctgactt cctgtcagcc ggtgaagctg ggcttgccgg
    10561 gtgactttcg caatctatac ctcgatcggc tgagcggtcg gatgggtgtg gtcgcattcg
    10621 ccaattcggt gcggaatccg gacaaaggcg ggccgacgcg gaatcccaaa cagactccgc
    10681 tgccgcttga gccagcgcgc atctgggctt gggatcgaaa cggacagccc cgtctttccg
    10741 tggatggttt ccgtcatgaa gacctgctga cgatcagcga catcagctgg agtccagatg
    10801 gcagcaccct gttgattgtt ggtcgtgaaa gtcgcgatcc aggtgttgaa aaactgagtc
    10861 tgtggcggat cgaaaacgga gcacttggca agatcccttg ggcgccttcg accgtgacga
    10921 tgccgctttt ggcaccgccg ccgccgccac aagcgcgcgc gggttcccaa acgccacagc
    10981 gatcgaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc acagttttcg
    11101 ccggacggtc gctacatcgt cgcttgtgac aaggaacgca gagttctttt gtacgagcga
    11161 agtgcggagg gtccgtccta ccgcgtgtca gcggtactgg gtccgtttgt gcgccagtgg
    11221 acgcgctgct tctttggggg tgatggaaag cggatcttgc ttgggtacag cggggaacca
    11281 ggactgcatg tctttgatac cgcgggtcac tttgtgaccg acatcgccga tggattccca
    11341 agggttcttg tgcgcacgga ttattacgtg acccagcggg tttcatcaga gcgtctgctg
    11401 tggcggtttg ctcgtccacc gattccgatg ctgtcgagcg cgcaagacaa tgtgaacttt
    11461 gccgtgaccg accgcaatga aaccaagctg gtcacggcat cgggaaacac cctgtttttg
    11521 tggaacgaca aaggcgaaca agttggtcgc acccgaagtc tcgcagacca agtgatgtcg
    11581 ctgcgaatca gtgatgacgg ccagcgggtt ttggcgatga catcgtcgga tcgcatgcgc
    11641 tgttggctgt gggatttgac gacagacagc gatccggtgg ccatcgatac cggtgatgaa
    11701 tcggtctact gtttcatgtt ccaccacgaa ggaaagacgc gcattggcgg actcagtggt
    11761 cgcggagaag tgtggcagtg gagcgattcc ggcgtgcgtc taccagtgac gcttgatctg
    11821 aaattggctg cggcgcaagt gccctctgtt cgggtaacga tctcctaccc acgcgcgggt
    11881 acgtacttgg tcgtgcatac cgatggtgcg acgcgcaagt cgcttctgtt cgattggaac
    11941 ggaacgctga tcagggaact gccacgacag ggagattcct atctgctgga tgctgcggat
    12001 catggggatt tctttgtcac gctgtctggc cagacggtgc tgcgcttcga cagtcgaggg
    12061 aatccgttgc cgctggcgat tccaaatgga atgcgcgttg cagacaagcc gcttcttacg
    12121 gtaagcgaca cgggccagta tattgcgctc agcggatcgc tttcggaatc gcccggcgtt
    12181 tggctttggg atacccgcgc aggcggtccg ccacgtcatc tgccaacgac gtccggagtc
    12241 cgcaagatcc tgatccatga aacgagccgc gccttggcga tggaaacgga acagaagcga
    12301 gttctcattt gggatctgtc caaagacatc gacaggccgg tgcggcgcgc cagtggcagg
    12361 ctgattgcgg ttgctgcgga agttgcgaat cgtccgtggc tgtttatgca agaacgcgat
    12421 ggctcactgt tgatgtggca tgtccgcgat ggcgtccatc atcgcttcgc aacgctgcgg
    12481 gaagactggt cgatagacga tgtgctggtg agtcccagcg gtcgatttct ggtcccgctg
    12541 agcaccgttc gtttcagcat cgactcaagt cgtcgggttc gcacaagcca tgtgtggccg
    12601 ctcgatgaat cgcggctcag cacgcagatg gacatggtgg tgaagtcacg cagcttgcgc
    12661 gatgcagatg atggtgccag ccaagaccaa gaggatgaag agcaagaaaa ggatgagctt
    12721 ctcaatcagc ttggtgactg attgcgcgaa aggggattcc ttccggtgtc tgaatcgctg
    12781 ctctacttta acggcgtaaa cgcgctcact ggtgactatg cacgggctcc tgcatctgtg
    12841 cagggatggt cggcgcagac actttcggtt ccaagcacca cgctgttggc cgatctggaa
    12901 aagcgcaaag aacagaccga acagagcgat gggaagcttg ccgcggcaag gaaacactac
    12961 gctgagctgc aagccgactt ggcgacgctg ctgcgcatgg gatcggcgga tgtgaaccag
    13021 cttcaatcgc tgcgcagaca gattgcagat gccgagcgct ttctggttcg caacacccac
    13081 gcaggggtgc ggcagggcat cgatccgctc tctttgtcgc aagcaggatg ggggatcatt
    13141 tttccggcgc gcatcgcagc ggatcggcaa agtcagattt tggaagcgtt actcccgctg
    13201 ctgtcccggc gcaagatgca ggccggggat cgctttcgaa cctatgtcgg tgctggcgga
    13261 tatcgaaccg gtgacaccaa agcgaggttt ctgtcccgtc atggggccgc gcctgctggg
    13321 ccggcatcgc cggaagcggt tccgtattat cttttgctga ttgggagtcc ggaagagatt
    13381 ccgtttgagt ttcagtatca cctggacatt cagtatgcgg tgggacggct tcactttttg
    13441 gacatcgcgg gctaccacaa ctacgcaaag tccgttgtac agaccgaaga gaaggggagt
    13501 cccaaagcgc agaaactgac gctgtgtggg gtgcagaatc cttctgatcc agcgacggaa
    13561 aagaccgaac gattcctcac caagccactg tcagatgagt ttgggcagac agcgcacggc
    13621 tggcagatcg aaaagctggg tgcggaacag acttcgcaaa aaggaatctt ggcccagctt
    13681 ggtgggtcac agactgcttc gcttctgttt gttgcgtgtc acggaatcga gtatccgacc
    13741 ggacatccgc tccagcgtgc acggcaaggg gcgctgcttg gcaacaggtg gtgtcgcagt
    13801 gaaaatcggc ctctttccga agacgacttc gttgcggggc atcatctttc cagcagtgct
    13861 gatgtgtccg gaatgatgct gtttgcgttt gcttgttaca gcgcgggggc gccgcttccg
    13921 gcggaatatc catccccaga cggtatcgtt ccagcgacgg cagcagatgt tccttttgtg
    13981 ggcagtctac cgatggaact gctgtcacat ccacgcggcg gtgcgcttgc tgtgattggt
    14041 catgtcgagc gcgcgtgggg ttttgcctat ggtgttgatg atgcgcggca acgatcccac
    14101 accgcggtct ttcacagcgc gctgtcgcgt cttttgctgg gccatccggt tggtcatgcg
    14161 atggaatttt tcaacgaaca ctacgccgaa agagccacgg cacttagcga gctggtgttc
    14221 cagattctca accaccgcac ctacgatccg cagatcctgg gcgagctgtg gtgtgagagt
    14281 gccgatgcgc gtggctacgt cgtgttggga gaccccgcag tccgttttgt ctatccaagt
    14341 ccttcgctcg cagaagccgt tccgcaagtc gcagaagccg ttccgcaagt caccgacatt
    14401 cgcgatgaac agacagcatt tccctgtccg caaggaattt ccccagccga ttgggaacgc
    14461 acgccgcgcg cggtaaagga gtgggccgct cgcttgcaaa aacagtcggc ggaaacgccc
    14521 gtgcgctgat ttgtgaaggg gtctactgaa actgctgggt gccgctgacc gtctggccaa
    14581 cttgcagtgc aatgccgctt tcgaccactt gctggccgct ttgtgcggtg atgcggaagg
    14641 tgtacggacc ggggcctgga tcgggcgtgt cggcgacgta atagttgaac gtctgcggct
    14701 ggagcttcac ccagtttcca ctctgctgaa gctcgatgtc tttgattggc agtcgactgt
    14761 tgcggacttg caccgccatc caccaagcgt tcgagccttc cttgaagtga attcccagcg
    14821 gaccggacac cgcgcaggcg acgggagtcc agctgatttt gacccgtccc gccgacttgt
    14881 cggcaatcat tgtgaaggcc tgttcggaca gatcgagatc gccacttttg cactctggac
    14941 acagatcaac aatgcgcacc gtgacggtac ctttggggcc agtcacatcg acgcaggtgc
    15001 cacagacggc gctggcagcc cactgcggtg cattcatcgc cgccaccatc aagttggacg
    15061 gagacggatc atacgaacaa gcgcctgcgc cggtcgctcc gtaatacgtt ccgtcgcccg
    15121 tctgcgtcga tccaatctgc ggttgcgaac ccggcagctg cggaatgtcg gaattggtac
    15181 tgctgttgct tgtgacggtt ccacacccca caagcagaag agaaaacgcg atgacagagc
    15241 caccaaagcg tgcaccaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    15361 gtgcaatcga caagctcatc ggggactccc tttttcacta gtccaggcag gtcggaagct
    15421 ttttgggggg cggaatccgc ggctttagct ggctctgtcc cttcatcacg accgcgtgac
    15481 agccgctcat gcgctgggtt tcgctgcaca cgccttcttc gcgcgcttcg ttgtcatagc
    15541 ggcggaacag acacagacct tggcattcgt caccgtcgtg gcattccttg ccggcatcgg
    15601 tggttttgca gttgcagcct tggttgtggt ttacgccgta gcgaccccag acaccgctgc
    15661 atttttcgca ggcctctttg gcttgggcga gcgctttgtt ggcaacgtga atggcgctgc
    15721 ggacggactg ctcgctggtg ctgccggggg cgcggaaata gatgacgact tccagctcac
    15781 cgggcgatgt ccagggaatc gcttctttcc agacgctgcc gtttggcacg atcggcgccg
    15841 gatcgaactc tagttcgagg ctgcgacggc ctttttcgta catttccatg cgatacaccg
    15901 actgaaactc gccggtgcag tttttggcgc gcagcggaat gtcactttcg catttgtccc
    15961 acgtctcgtc gcacttgagc gcgacttcag tgggctgcat cacaaagccg ctttccatcg
    16021 gggagcagcc tttgggcatc tcggcaggtt tgggcgcggc gggcttgggt gccggcttgg
    16081 ccgcgggttt tgctgtcggg gccggcggcg ccgcgagcga cgagccaacc gacagaagga
    16141 ccagcgccga tgacaaaagc gtgtttttgt ccatatcaga gctttttagc atgccgcaaa
    16201 gtggggtggg tccgaaagcg cggaaagcct agcttgtctc gtccgacggc ttgccacgta
    16261 ccgcacgcgc ggtttcctgg tcgtcttggt ttcccaccgc gatgcccaca gttggcgtgg
    16321 ccaagtcgtc gtcgtgatcg tcgtcgtcgc tttcatcgtc tgcatcgtgg tcgttgagcg
    16381 gctcatcgtg atcgtcgtcc gcatcgctta tcagaccggc atcgctcgtc accaaaatgc
    16441 ccatctcttc gcactcgagc ttggcgcact gacgcagaag ctcttggctg acttctccgc
    16501 cttgcgccag cgcccgcagc gccgcccgca ccactgcgtt tttgatcgcg ccacccgaca
    16561 gatcgaactc actggccagc gctgcaaaat cgactccgcg ctcttttggc gtgtctttgg
    16621 gcagcatggc ttgccacatc ttggctcgct cgtattcgct cggcagcggc agggcaatcc
    16681 ggaaacgcag ccttcgcttg aacgcctcgt cgagcagttt ttcgttgttg gtcgtcagta
    16741 tcagcatgcc gtcgtgcgat tcgaccgctt gcagaagaaa gctcaccgcc atgttggcgt
    16801 agcgatcctg cgacgaacga acatcgagtc gtttgccaaa caacgtatcg gcttcatcga
    16861 acagaagcgc gacttgttgc cgcttggctt cgtcgaaaat ccgcgccagg tttttttcgg
    16921 tgtcccctgc gaatcgctcg acgacccgcg ccacgttgac gcggaacagc tccaggccga
    16981 tttctttggc caccagcgcc gccgccatcg tcttgccggt gcctggcgcc ccggcgagca
    17041 gcacgctgat cgatcgaccg tagggcagaa gccgttcaaa accccacttg ccgagcagct
    17101 gttcgcgata acgggcgtaa gcgaccactt cgagcacgcg atcgagaatc tcggcaggca
    17161 gcaccaggtc ttcccacgac agcgatgtcg tcacaagctg cgccaaatca ccaaagcggt
    17221 tgccaagctg tttgcgaatc gcttcccgcg ccgctgccat cgtaagctgc gggcgacgac
    17281 caccgtcgat ccgggcacca ttgactatct cgctggcgct gcgctggatg gccccggcgg
    17341 gcagcgaata gtgactggcg agcacccgca catcaaagcc tcgctcacgg cccatgtcga
    17401 cgggcagcag actgttccac agcgccacct gggtcgcgcg atccggcatc ggaaaatcga
    17461 tgcgcagcag atcgggcagt cgcgtccaca gcgcatcaat cggtccgtcg gcggccagca
    17521 ccacccgcac ccgactcccg agcagaagcc gacgaagctg ctcaaacagc gacggatcga
    17581 gctctcccaa aagctctgcc gaggcgatgt acaaaagtcc ttgctgcagc gccgcttcgc
    17641 cgagcgccgc ttgcaccgtc ggcaacagac tggctcgttc gcccagcagg cttgtcgccg
    17701 aaagcaccac caaaggccgt ccgggaagca gcgccgcaag cagcgccttg cgaccactgc
    17761 gccgcagtcc ggtcagacac agcggcggca gcaccggccc gtctggatcc ccgagcagcg
    17821 gctgcgcgga ctcgtgcagc gcatcgcgca ccctttcgag cagccccgct ggcaacgtga
    17881 gctgggccag cgtccgctgc gcaaaataca ggctgacgcc gtgtggcagc gtgtgcagct
    17941 tgggtctgtc ttccccgcgc aaaaacgctg ccagtcgatc cgacagcttg agccgctgaa
    18001 acaagagcgg cgtcgttggt gcccacgctg catcgtctgc caacagcaaa aagccgtgtc
    18061 gtcgcagcgt gcttcccggt cgcagcgctt cttgaatttg tggttcgggc agtccttgct
    18121 ggcgcaaaag tgtcaggtaa tgcgcgacgg tgccttgcgg atgtggacca aaaaacggtg
    18181 ccaggatctg ctgcgctgct tcttcggttt cgcgaagcag cagcagggtg acaagccgca
    18241 cttccgcatc ggacagcgac agcgactggg ccaggcgcat catcggcagc gtttgaccac
    18301 ttgcatccga aagcgacagt cgctcgcgaa tctgaagcga cagcgtcgtt agcatgtcga
    18361 taagttccgc cttttcaggc tgcgcatcgt ctgtgtccgc cagcttgtgc agtcgcagaa
    18421 gcagcgtcag acggtctagc tccgattgca ggtgttcgcg gaccgaaaca tagggttggc
    18481 tgggcagggg cggggtgtgc agcatggcaa aaaaacctag ccgcccgagc atatcacgac
    18541 ggagagtgct tttaaccgtc cacccaagag cgattttgtg cagtagaatg cgcaccgtcc
    18601 ttctcgacct ggtgaggcag accacatcca tggcccaaga gaccacgccc aaaaccaagc
    18661 cccgacgggc tacgattgtc tctccgtcga cagcggcgaa agctgaagac gcagccgcgc
    18721 ccggcgccga acccgagctt gtagaaggcg gtgccgccga aagtgccgaa ccaggcgaag
    18781 acgatgccga catcgaaagc ctggtcgggg ctgccgtcga tgcgatgccc gatgaagaca
    18841 tcgtgctggc tgccgatgcg ctcgcgccag agattctgtc ggacggaaaa cgcggcggct
    18901 cgctggtcaa aagcgatccg ctgacggtct acatgcacga aatccggcgc tatccgctgc
    18961 tcgatcgcga tgaagaacac cgacttgccg tcgagtattc gaagtcacgc gatccgcgaa
    19021 ttggccggcg acttatcacc gcgaacctgc ggctcgtcgt caaaattgcc cacgaataca
    19081 agcgggccta tcggaacctg ctcgacttgg tgcaggaagg caacctgggg cttttgcaag
    19141 cggtcgaaaa atacgatccg catcgaggcg tcaagctctc atcgtatgcc gcgtggtgga
    19201 ttcgggcgta catgctcaag ttcatcctca acaactggcg gctggtcaaa atcgggacga
    19261 cgcaggcgca gcgaaagctg tttttcaacc tgagcaaaga gcgcgacaag ctcgaagccg
    19321 atgggtttgt cccgacgagc aagctgctgg ccgagcggct gcatgtgccc gaacaagaag
    19381 tcaccgatat gtcgctgcgg ctcgggcaaa gcgagacgtc gctcgatgcg ccggtcggtc
    19441 gtgacgatga aggcgggcgt cgacaagtcg atctgctcga atcgcctggc aacacccggc
    19501 ccgatttgac cgcggaaagc gaggagtttc gcgcactgct ccgcagcaag ctgtcggagt
    19561 ttgaacagac gctgagcgga cgcgaaaagc tgctgtttca ggaacgactg atggccgatc
    19621 agccgctgac gcttcaagat gtcggtgagc gctttggcat cagtcgcgag cgagcgcggc
    19681 agctggaagc gcggcttttg ggacgactca aagcgttttt gaaaaaagag ctcggcgacg
    19741 cggtgcaggt cgcgatgggt ctggaatagt cggcgggaac aatcaggtga gccggcttag
    19801 cagtgtcttg gccagctcgc gaatgtcgtc gatgggattc gtctcggtca gatagcgcag
    19861 cttgccgatg agcgcgcgcg tccgcttggg cagatggcgc ttgtcgagca gctcgccaat
    19921 ccgggtcatc gcttcgcgaa tccgtgactc tttttcgtgt tccagcagct gctccaggaa
    19981 ctcgtgatcg tcgggaatcg gaaaccgagc caggtatttt tcggcggcgc gactgatttc
    20041 ttgccgattg acggcttcgg ccagcttgcg cagaaggctc gctttgtcct cggtcggctt
    20101 tttcttggcc gccgtctttt tgccgccctc cgcaggcgcc gccgcttccg ctggattcgc
    20161 tttgccatca gacgctgccg ccgccggagc tgccgcaggg ggcggcgaag ccggcgcgac
    20221 aggctttggt tcttccgccg gggtcgagcg tcccgtcagg ttcagcttgt ccgcgagttt
    20281 tcccagctct cctttggcaa atgcggcttc gagcgcggcg cgatactgct tctgccgcgt
    20341 cgcttcctca tggtgcgggc gttccgacgg cgctgcgcct cggccagtgc tggtggttcc
    20401 gccagcgcga cgcttatcga tctctcgcca cgacagcttg ggtcgatctt cgtttcccat
    20461 ggtcttctac tctcgcgaaa aaatggtcct aacccagctc gtgacgcggg ggacggccca
    20521 gaaggtcaga catcacttgc ccgacgggtt tgccttcgta caaaacacgg tacatcgccg
    20581 cactgatcgg catttcgacc ccgaccttgg ctgccaggtc gtgcaccgag cgcacggtgc
    20641 ggattccctc ggcgacttgc ttcatttcgg cctgcacctg cacaagcgtt ttgccctgcg
    20701 cgaacaaaag cccggcttgc cgattgcggc tctgcggacc gttacaggtc agcaccaaat
    20761 cgcccatgcc cgcaagcccg gccagcgtca gtggattgca gccgagccgc acgccgagcc
    20821 gggtgatttc cgccagtccg cgggtaatcg ctgccgcccg cgtgttgtga ccaaagccca
    20881 gaccgtcggc cattccacag gcaatcgcga tgacgttttt gactgcgccg ccgacctcag
    20941 ccccgatgac atcgtccgat gtatagacgc ggaaataacc aacctgaaag gcgcgctgca
    21001 ccgcttccgc aatcggacgt tgtttggcgg cggccacgac ggcagttggg tatttttcga
    21061 tcgtttcttt ggcaaagctt gggcccgaca ggaccgcgat tcgatcgtga agatgagacg
    21121 gcagaacttg tgaaagcaca tcgtccatcg tcagcaaggt gtcgttttcg attcccttgc
    21181 tggcggtcac gatgatggct tgcggcggaa tctcggcggc gatgtccgca aacacctgcc
    21241 gcatcgtgtg cgtcggcacc actgcgacaa tcagcgaagg cgcgtctttg ggcagcgtcg
    21301 caatcgccgc atgcagatcc gctgtgaagc gcagcgaatc agggagcctg agacccggca
    21361 gatagcggcg gttttcccgc gtcgtttccg cttcggacat ctgcgccgcg tcacgacccc
    21421 acagggtgac tttgtgctgg ttttcacaga gcacacaggc cagtgcggtg ccccagctgc
    21481 cggctccaac gacgacgatg tccatggtga atttccgctg ctcgttactt tgggatgttt
    21541 gggtctactg ctaacggtga gagccggtga ggagacgctc tcaaaaaggt caacgcatcg
    21601 aggggttacc tggggaacca gggctcgcga gagcaaccgc taaaccgtga ttcatcataa
    21661 caaaaagcgc cgcgctttcg tgctagcaaa atgggcccga tcatcgggac gctgcgtttt
    21721 ttcggaaacc gccgcgcgcg ctcagaccgg gccgcgcgat tgaataaaaa agtttcgggg
    21781 ggacaagtcc gcgatgcaaa aactggagtt gcccgcgcgc cccgttctgc ggtgggggag
    21841 acaaactttg ccgcaggcac ctgtacttag tcgtgttaac ctccaaaaca tgtcgcgtaa
    21901 gttgcttcac agtttggtcc tctgctccgt cgtgatggca ccgctcgccg cgctggctga
    21961 tgaggcggct gctcagtatc agaagggctt gcagtttatg cagcttggca agctcgatga
    22021 tgcgcaagct tccctggaag aagcgctgaa gctgcgtcct ggctatgccg ccgcggaact
    22081 taccttgggc aacgtgctgc gcaaaaagaa gcagtgcgac aaggcgattc cgcactacga
    22141 agccgccatc aagcttcagc ccgacgacca cttcgcgcac ggcaacttgg gtttctgtct
    22201 cgcgacggcg ggcaaagacg cggacggcat tcggatgctg gccaaagcca ccgagcttgc
    22261 gccaaacgaa gtgcagtggc agtcgtcgct cggtgcgctg tatcggcggg ccaagcagtt
    22321 tgacaaagcg ctgccgcact tggaaaaggc cgtgcagctt gatccgaaca atcccgacgc
    22381 gctgcgcaac tatgcggtgg cgctgcggca ggccaaaaac ctcgacaaag ccgccgaggt
    22441 gacgcgccaa gcgattcggc ttctgcccaa tgacagcaac ctgcgctttg accttggcgt
    22501 gatttaccgc gtccagggca agaccaccga agcgattgcc gcctaccgtg aagcgatcaa
    22561 gctcgaagac aaaaatgccg aagcttggta cgacctcggg gtgctgcttt cgcaggatcg
    22621 gcaaaagcag gaagccatcg aagcgtttaa caagtacatc gacctggtgg gcagcaaaaa
    22681 cccgacgcag atcgaaggcg tccgtcagca gattcgtggg ctaggcggca ccgcgcacta
    22741 gaacacttca gcgaaccacg agcgcatgcg acgccagacg ccgcggcgct cgctgctttg
    22801 ggatagctga agcccatcct gcttgcgcgc accgtacagc accagcccca ccgccgtcgc
    22861 gtagatcggc gtcttgacca catccgacag cccgcctatg ccctgcggta caccgcgacg
    22921 caccggcatc cccaggatct cttcggccag ctcgggcaga ccttgcaaaa gcgtcgatcc
    22981 gccggtcacg accattcccg acgccagcat atcggacaga ccacagcgct ctagttcatc
    23041 gcggaccagc gaaaaaatct cgtcgacacg cggctgcaaa atatccgaca gcactgtgcg
    23101 tggcatgacc cgtggcggac gaccaccgac cgacggcact tcaatcgttt cgtcttcggc
    23161 aatcaggctc gatagcgcgc agccatgctt gtgcttcagc cgctccgctt cgctcatcgg
    23221 cgtgcgcagc ccgaccgcaa tgtcgtttgt aagctggtgc ccgccaatcg gcagcacgat
    23281 ggtgtggacg agcgcgcctt cggaaaagac gaccagatca ctggttccgc cgccgatgtc
    23341 gagcagcgcc actcccagct ctttttcatc gctgtgcagc actgattccg ccgtcgcata
    23401 cggttccagc accaagtcct gcacttccag accgcagcgc gtacagcact tgatgatgtt
    23461 ttgcgccgac gcgctcgccg ccgtcacgat gtggactttg gcttcgagcc tgaccccggc
    23521 catgccgagc ggttctttga tgccgtcttg ctgatcgatc gaaaactcgc gcggcacgac
    23581 gtggaggatc tcgcggtctt gcgggatgct gaccgccttg gccgcttcga tgacacgctc
    23641 gatgtcacct ggccgaacct cacgatcgcg aatcgccacc acgccttcag agtggaaggc
    23701 tttgatgtgc gaacctgcga tgccggcgta gacctgggtg atctcgacgc cagccatgtg
    23761 ttcggcttct tcgacagctt tggcgacggc agcaacggtc gagtcaatgt tgacgaccac
    23821 gccttttttc agccctgcgc agggctgagt cccgacgcca atgatgtcga tgccgtcttc
    23881 cgtgatctcg ccgacgaggc acgccacctt ggtggtgccg atgtcgagcc cgacgatgat
    23941 ttcctcacgc tttgccatgg gtagtgaagt gtctagcttg ccaatgcccg tgcaaaggcg
    24001 cgaatttttt ggctaggtat ccagttcgac tacggtcact ccgtcgccgc catcgccggg
    24061 ttccgctgga tacagcttgc gcacaccggg aaacgcgaca aagtgttcgc gcagcgcgct
    24121 gcgcaagatg ccggtgccgt ggccgtgcag gatgaacacc gccgggcgtc cttcgcgcag
    24181 cgcatcgtcg acaaattttt cggtgcgagc caccgcgacc ggcagccttt caccacgcag
    24241 atcaatcgtt gcatccgggg ttcgcggcgg aatgatcggg gccgatgtcg ccatgaccac
    24301 ttggggcgga tgtctgtgcg ctttcggcga cggggcaggg cgatcagacg atccgcccag
    24361 gatcagcagt tccgccacgt ccgcagtgag ccgcagcgcc ccgacctgca cgacgacttt
    24421 tccgcgcgac acatcggacg ccaccactcc gctggccgaa agtcgcgtca catagacccg
    24481 catcccttgc gtcagctctt cggcgctcgg cacccgtccg actgggccgg ctttttccgg
    24541 aaccagaccg cgcaccgact ggctcagctc atctagacgc ttgctcgctt gcgacagttc
    24601 accttgcgca ccgtcgctct ggccgtcttc ggtcttgtgt cgcgtcgctt tgagcgcggc
    24661 cttcagatcc gacagctcgc gtcgcgcgga ttgcagcgtc tcgatcgctt cgtcataggc
    24721 tcggcgtcgc gctttttgca gttcgacccg tgcatcgtcg tgggcgcgcc gtgcttccgc
    24781 ggcttcactt gcggcaatcc gacgcaggcg ctcggcttcg gttcgcatcg tctcggtgcg
    24841 ctcgcgttcg tcggccagcg tttgcatcag ccgttcgacg ctcgactgtc cggacttgag
    24901 cagcgactcg gcgcgccgga tgagcggcag cggcaggccg agccgttccg ccaccggcaa
    24961 agccgacgac ggacccggaa ttcccaggtg cagcttgtac gtcggcttga gcgctttgag
    25021 atcaaatccc acgctggcgt ttttgaactt cgtcgactcc gccgccatcg ctttgagccg
    25081 gtcgtagtgc gttgtcacca gcaccgtcgc gccgcgcgcc gcaagtcctt ccagcaccgc
    25141 ttgcgccagc gccgcgcctt gttctggatc ggtaccgacg gcaatttcat cgagcaggat
    25201 cagcgtgccg cgatccgctc tgtttaaaaa ttcgcacagg ttcagcacat gcgccgaaaa
    25261 cgtcgacagg tttttttcca ggctctgatc atcgccaacg tcggtcagta ccctgtcaaa
    25321 ccagggcaag cgcgaccctt cttgcgccgg cacatgcagc cccgcccgtg acaagagcac
    25381 gcacagaccg gtcagcttca gcgcaacggt cttgccaccg gcattcggtc ccgaaatcac
    25441 cagcgccgcc cccgcatcga gcagcacatc gtttgccacc acctgcacgc ccgacagcac
    25501 catcagcgga tgccgtccgt tgcgcaggtc gatctgggcg ggtttttccg tctgttcgct
    25561 ggcatcgacc agttcaaccg ggcttgctcg cagatccgac gccagcttgg ccgcaccatc
    25621 catcaagtcc aggtaggcca agatttccag gttgcgcagg attctgggcg atgactcttc
    25681 gacgagccgc gacagctcgg ccaaaatccg tcgttcttct tccgcgacct caagctgcgc
    25741 cagcttgagc cggttgttta gttcgatgat ctcttcgggt tcgacaaaga ccgtggctcc
    25801 cgatgcgctg acgccgtgga cgatgccgcg gatcttggtc cgggcatcgg cgcgcactgg
    25861 caccacatag cgttcttccc gctgggtgac aaagcggtct tgcagatacg gctcgatgtg
    25921 tccttcacag agcaggctgt cggtgcgacg cgacaggtcc gaaagcagtc cctgtacccg
    25981 ctgccgcagt ccgcgcagtg ccggtgaagc cgagtcgcgc agcgctccat gatcatcaaa
    26041 cgaatcgctg atcggcccgg cgacttccgg cagctcggca atcggttccg catgctgtgt
    26101 cagcgttgga agccgtgccg atcgggacag aatgtggcgc cggagccttt gtcccgctgt
    26161 cagggtcttg ccaatctcgg tcagctcatc ggggaccagc acaccggctt tttcgcttcg
    26221 ctcgacgaac gcccgcacgt cggtgattcc accaaacggc agcgcttcgc caccatcttg
    26281 cagtgcccgc gcttcgctga cttcacgctg gcgtgtctgc gcctgcgcaa tcgatccaca
    26341 aagcggcagc tctttcgcaa gctgcatccc ccgtgtcgtg tgacagcgct ctgccagctt
    26401 ggccagcagc gcggtccacg acagatccgc caccgtcttt tcctgcacat gcacatcccg
    26461 catcgtgggg cgcatcctag caccaccacg gcgggactgc ttttttgggg acgaaaagct
    26521 tacagctcgc cgctcttggc gatggcgatc tttttcgtcg tccggcccga gtcggtgccg
    26581 cacgcttcga tcttgcggac cagatccatg ccttcgacca cctgaccaaa cacgacgtgc
    26641 ttgccgtcga gccatggggt cagcacggtg gtgataaaga actgcgagcc gttggtgttt
    26701 ttgcccgcgt tggccatcga caaaagaccc gctttgtcgt gcttgagctg gaagttttcg
    26761 tcttcaaact tgtgaccgta gatcgaacga ccgccggtgc cattgtggtt ggtgaagtcg
    26821 ccgccctgga gcatgaagct tggaatgacg cggtggaaga tgctgccctg gtagtggcca
    26881 gcatggtgcc cgacgcacag cgcgcggaag ttttccgccg tcttgggaac cacgtcattg
    26941 aacagttcaa acacgatgcg gcccgccggc tggtcggcga tggtgatgtc gaaatagacc
    27001 ttgggtctgg ccatagcgct tgctctccgt ttgaggggta tgtttcgtca gcaacttgtc
    27061 gtgatatcag tcacgacaga aagtctggcc aggatagcgc aacttgcggg tctgtgagcg
    27121 gcttttggtc ggttctagtc ggcgctgggg accgcaagtc gggcgagcgc ggtgcggatc
    27181 gaatcgggaa tcggcgtgct cgtgcgtgtt tcgcgatcga cgaacacatg gacaaagtgg
    27241 ccttcggcag cagcgggctc actgtctttg tcggttgtct gggcaaacag accgatttcg
    27301 taacgcacac tgctgcgtcc cagatgtcct atccgcagtc ctgcatcgat gatctgcgga
    27361 aacgaaagcg gcgcgaagaa tcggcactgc gtttccaccg caagcccaat cactgcgcca
    27421 tggtgaatgt cgagtcctcc ctgcgcgatc aggaattcgt ttatcaccgt atcgaagaac
    27481 gaatagtaga cgacgttgtt tacgtggccg tagacatcgt tgtccaaaaa gcgggtcgga
    27541 atcgacaaaa aatggcgata gtcccgacgc agactgcgcg gcgtagcgtt tttttgcata
    27601 ggtggtggat ggtgtttcat ggccctgctt cttgtacgga tcaggaagca aaggaatcgc
    27661 gaaacatgac ttcaagatcg ccgggtccga cgtggcgcgg tgcattccca agcagcctgg
    27721 tttgcagcgc tgcgccttca cacagcgcgg gtacatcggt gagacaatag cctgcttggg
    27781 acagctcgct tgggagtccg ctttcgccaa ggagtcggcg cagctcaccc gataggaatc
    27841 gaccagcgtc ggcatcggcg atgtctctgc aatcaagccc aagcgcctgg gcacagcgca
    27901 gatgacgccc cggacttgtc gccgccagat gaaacaccgc agccggtgca gacaacacca
    27961 ctgccagacc gtgcggaacc agcggccttg tttgcgggta gcctgaaagg gtcagcgtgg
    28021 gagccagtcc tcctatcgca taggccatcg catgggggac gtgaaccccg gcattcccaa
    28081 acgcgattcc tgccagtgct gccgcataca tcatctggtc gcgcgcttcc cggtcctcag
    28141 catcgcgaat cgcccgcaaa agataggttc cacacaaccg cagcgcttcc agacttccga
    28201 catccgaaaa cggattggca ccttggctca tcggtcgcgc aagcggggat tcgggccggg
    28261 cgcggcgcga aaaaggaagt gcggtatacg attcgatggc gtgacacagc acatcaaaac
    28321 ccgtcgctgc gaccacatgt gcaggcaggg tttcggtaca cacgggatcg atcagcgcca
    28381 gccgaggccg cagcgcccgg tgcgcaagac ctgtcttgca atgccgacct tctgtgccaa
    28441 aaagaggcac actacagacg gcgattccgg tgcactcact gccggttcct gatgttgtcg
    28501 ggcaggcgat gtgcggtggc agcgggcccg gcagcgccac gccttcaccg atcgggcgat
    28561 tgacatagcg cagaaacgga gccttgtggg ttgcatacgt cagcgccgct ttggcggtat
    28621 ccatgaccga tccgccgccg acggaaatcg ccccatcgaa gcgtccttcg gcataaaact
    28681 gtgatgcttg ttcaaacgag gcttggcttg gttcaatccg cacctcgtca taacagacag
    28741 aatcgattcc ggcggcccgc agcgactggg tgacaagctg tactggcggc agcttacgca
    28801 ccaccggatc ggtgaacagc gcgacccggc gcagacccag actttttgcc agctcgcctg
    28861 cttccaaaag cgcgccgcga ccaaacacga tgtttgatgc atcgaccgca aaacagccat
    28921 cgccgccttg tgcctgggca aagtagtgcg cgcagctcat gctcgcgcca tcctacaagc
    28981 cggtcaggcc tggcaataag aggtatttta catgattctt attgggtccg caccccttgg
    29041 cggcatgccg tgcagaggat gcacgttggg gctggtttgc ggaagcgatg caggtcaggc
    29101 ggcagcgcgc tgcgaggcca ggaacttaga tgtcgaggtc ttttgccggc agcgattttt
    29161 tggccggagc gcttgggctg tcggcaccgc cgtcgtccag ttcgacaaac agaccgtaga
    29221 gcgccccaat gatgggggcc aacaccgtcg ccgactggat cgacagcttg ttggccactc
    29281 cgtcgatgct caggctcaaa gccggcataa agcggtgccc cactgcatac agcccgacgc
    29341 caatcgcggc gccgaacacc atcttcagaa tcggtgtcca cagcgttgcg gctttccagg
    29401 gggccttgcc acagacgacg ccgacggttg cacccagcag cgcacagaac aagtacgcca
    29461 ggatgcccgc tggactgcct agcttgagca gcgcgaagcc aattccgccg ccaaccagaa
    29521 ctcccttgat aaggccaagt agtgcgcgac ccatctgccg ctccttgtcg aaaacgcgaa
    29581 tgtgtttcta gtatagcctt tcggcccggt cggcaaccgc tgctgcgcat tgtgcccaga
    29641 tgacgcgggg cccgcacagt ggttgtgact gcttgtttgg atgctggtcc ttggccctac
    29701 agcgtcgagc ccggatcgac atcggcatgt acacgcaggc cggctggaac tttgtgaaga
    29761 agtgttaggc gcagcagccg atgcagtgat cgcacgctgg gtgatcgcac catcagctgc
    29821 cagcggcagc ggcctttgag cttcgacagc ggtgcttccg cgggaccgag caaagacgac
    29881 ccatcgggtt gtgccgaaag ctccgcctgc aagatgtcgt gcagctcttt ggccacctgc
    29941 gcgactttgt aggggtcttc tccgtcgatg cgcaaaagcc cgagccggac actgggcgga
    30001 tacgacagcg caagccgcgc ctccagctcg gcttcggcaa acgacagata gtcatgctgc
    30061 gccgcgcacg acaccgccgg atgttccggg ttgtacgtct ggatcagcac ccgacccgga
    30121 cgcgacgccc gtcccgcccg acccgcgacc tgcgacaaaa gctgatacgt ccgctcggcg
    30181 gcacgaaagt ccggcagtcc catcccggta tcggccagca ccaccccaac cagcgtcacc
    30241 cctggaaagt catgcccttt ggccagcatc tgggtgccta tcaggatgtc cagctcatgg
    30301 cggtgcatcg cggacagcac ccgtgtcagg ctgccggcgg tgtcgcgatc cagccgctca
    30361 atccgcgctt caggaaagcg gagctgtagc tgttccagca gtttttccgt ccccagtccg
    30421 agcggcacaa gcgacttctg ctgacagagc ggacagtcct tgggcatcgg ctcgcggtgg
    30481 tcgcagtagt gacagatcag gctttgccga cccttgtgca tcgtcagcgt cacggcgcag
    30541 ttcggacact cgatgcggtg gccgcagctt tggcacaaaa gaaacgccgc gtacccccgg
    30601 cggttcagca gcaaaatcgc ctgctcgccg gcttcgacgg tggtgcgcag cgcttccaaa
    30661 agcggtcccg tcaaaagcgc tgactccgtc gccttgacgt gctgcttcag gtcaatcact
    30721 tcgaccttgg gcattgcgat tcccgtcgct cgtttgtgca gcgtaagccg ggtcagcttg
    30781 ccacttcgtg acagcgccag cgcttccaaa gacggcgtcg ccgatcccag caccgcaatc
    30841 gcaccggctt gctgggcgcg ccacagcgcc acgtcgcgtc cgtggtagtg cacgccgtcc
    30901 tgctgcttaa acgatgagtc gtgctcttcg tcgacaatca ccacgcccag gtggctaagc
    30961 ggcgcaaaca ctgccgagcg tggcccaagc gcaatcgaca cctcgccgcg caggatcttc
    31021 cgccacgcgc tggcccgttc cgcctgggtc agcgccgagt gcagcaccgc cacttgcccc
    31081 ggaaaacgtg acgaaaagcg cgccgaaagc tgcggtgtca gcgcaatttc cggcaccagc
    31141 accagcgccg tctgtccccg cttgcgcgcc tcgccgatga gctgaagata gacttcggtt
    31201 tttcccgatc cggtcacgcc atgaagcaaa aaaccctggt agccactgcg ccgcggcttg
    31261 gcatgctcgg cagccgacaa ggccaaaagc agcacctgga gcgcgtgcgc ctgttcgtcg
    31321 gtcagtacca gcccttgggg agcgctcggc agcgtgtgtt cgtcgacttt ggcctttggc
    31381 tctttgctta ggccgacctt tacatcgcag tggatctcga ccagatgccg cttgtgcagc
    31441 tcgccgagcc ggcttcgcca gtctgctttg ggccgtttgg acagtcgctc atgcgatagc
    31501 gccgtatcga ccgaaacaaa cgcctgcgga ctgcgccgac tgcccagcgc ttgcaaaagc
    31561 tcaatctcac gcgggtccag gtcggcgact tccggcgata acagcacccc agatagcttg
    31621 tgggaaagcc gcagtccttc gtcagtaagc tttacccgtt cgataagctg cgcatgctca
    31681 cttggcggca aaagcagtcg cagcgcttca ccgagcggtg acaaatagta atcggcaaca
    31741 aaccgcgcca ttccgagcag gtcggcgggc agcttggctc ccgacagccg catgtgcagg
    31801 ttgcgcagcg cgtgtcgcgc aaagccactc accgagagtg tctgtgtcgc cgccgcgccc
    31861 tcgatggatt caaggacgat gccttccacc cgcggctgac caccgagcgg caccagcacg
    31921 cgatcccccg gcagcggcat cgccgtcatg tcatcgggaa cgccatacag atacggaccg
    31981 ggggccggcg acatcaccgc gacaaggcac agcctgggcg acggttcctc aacaagcgac
    32041 ggctgggttt gctgcgccgc ttggactggc tccgcttcgc tcatacgtag tcgagcacca
    32101 tggtttcgta gtgccggaac acccgccagc cgataagcag cgccagcacc gtcggtatca
    32161 cgatgccagc ccagccgagt ggcgtcggaa attcaccgcg cataatcgcc atccgcatcg
    32221 ccgtcataag cccggccagc ggattggcct gcaggatggt gcggaacggg aacggaatca
    32281 catcaattgg gtagaacacc ggcgtcgccc agtacaaaag caaaagtccc gtgctcacca
    32341 gataggcgat gtcgcggtag atgacgttta gcaccgacgt ggccagcaca attcccgcca
    32401 tcagcgagat ggtcagaagc agcagcaccg gcacaagcaa aagcgcgggc gacagcttga
    32461 acgcgccagg gaagatcggg ataaacgcga tcagcaccag acactcgatc gaaaagttga
    32521 tcccgtagct gagcacgctc gcgatgggca gaagcggcct ggcaaacacg gtgcgcttga
    32581 tgatgtcggc gttgtggaca aacacgccgg tcgcgctgtt taggctggtc gaaaaccact
    32641 gccacatcaa aagtccgatg agcaggaaga tcgggaagaa cttttccgcc gagcggaaca
    32701 tctgcgtaaa gatcacgctg atgattccca tcatcaccag cggattgagc agcgaccaca
    32761 caatccccag cgcctgggcc cgatagcggg cgcgaaactc ggtccgcacc agcacccaca
    32821 gcacttgcaa caggctctgc cgactgacga gcgccgcggg ttcatcgggg gcttcgaccg
    32881 tcttttggtc tgggcttgcc ggcccgcccc gcttggcatc gagcggtccg tggtaaacgc
    32941 cgcggctggt tcggtgggta aagtcctcgg gcagcgcgcg cggtcgccgc tgttcgagag
    33001 gaatcacagg aggaacgtcg ttcaaggcgc aaatccttcg tttcgtaagc agtaagtcaa
    33061 agaggcggca gtttacgacg gctttttgcc aggataaagt gtcagtccat gcgagcactg
    33121 cgtactttcg ttcccattgc ttcggcgctt ctgctggccg gctgcccgtc ggaaaagccc
    33181 aaacaagcgg cttcgaccag caaacctgcg accccggctg ccagcgcttc tggcccaaac
    33241 caaggaaatc aatcaatggc acctgaacct ggcgatcttc cggctccttc tgatgtggcg
    33301 gcggcaccgg ctgatgccga aaagtcggcg tccggccttg cgagcaaggt gatcaccaaa
    33361 ggcaccggca ccgagcgacc cgaagcccaa gacaccgtca aggttcacta caccggctgg
    33421 atgacaaacg ggaagatgtt tgactcgtcg gtcaagcgcg gctcgccgac ggagtttccg
    33481 ctcaagggtg tcatccccgg ctggaccgaa ggcgtgcagc tgatggtggt cggtgaaaag
    33541 cgccgcttct ggatccccgg caagcttgct tacggcgaca gtccgacgcc gggtcgcccc
    33601 tacggcaccc tggtattcga tgtcgagctg ctcgaaatca agcgcggtcc caagccgatt
    33661 ccggcccccg aagatgtcaa agcggttccc gccgacgccc agaagacacc aagtggcctg
    33721 gccagcaagg tgctgaccaa gggcaaaggc acgctgcatc cgactgccga tgacaccgtc
    33781 gaggtgcact acaccggctg gatgaccacg ggtgaaatgt tcgattcgtc ggtggtgcgt
    33841 ggccagccgg ccaagttccc gctcggacgg gtgatcaagg gctggactga aggcgtgcag
    33901 ctgatggtgc agggtgaaaa gcgccgcttc tggattcccg ccgaccttgc ctatggtgat
    33961 acgccgcgtc ggcccggtgg cccgcacggc atgctggtgt tcgatgtcga gctgctcggc
    34021 attgcgcaag cgcccaaggt tccgcccgac gtcaaagagc cgcccaagga tgccaaaaag
    34081 acgccgagcg gcctggccta caaagtgatt gccaagggaa cggggcagaa gcacccaacc
    34141 gcgacgagca ccgtggaagt ccactacagc ggctggacca ccgacggcaa gatgttcgat
    34201 tcatccgtgg tacgtggcga gccgacgagc ttcccgctaa acggcgtgat ccccggctgg
    34261 accgaaggcg tacagctgat ggtcgagggt gaaaaggccc gcttctggat tcccggcaag
    34321 ctcgcctatg gggatacccc gacgcgcccc ggtgcgcccg ctggcatgct cgtgttcgac
    34381 atcgagctgc gcaagatcct cgacaccccc gcccactaag ccgcacggtc ccccgttact
    34441 ggcagatcaa gctgacggaa cgggtgccgt ttggccccgt caccgtcgcg gtgttcgttc
    34501 catcgaacat gaccatgacg gtggcgttgc ttggcgtcgc cacatgggtc agcatgccgc
    34561 ttgggcagcc atcgacacca tttggacggc agaccttgta gtctttcgtc gtcgtggcga
    34621 tcgacaccgt gcctgcggtg gtcgcgcccg tgccgttcac cgtgcgacac ttcgtcgccg
    34681 aatccaccgt cgccgtccac tggttgctgt gggtgacttg cacgccgttt gcattggtgc
    34741 gggtccacgt cgtgctgcct ttgacgtcgc gggtggtgcc ggtgccgctc acggtgatgt
    34801 tgcggtccat cgtgaacgtc gctgggttgc cgttgacggt catgttggtg ctggccgcct
    34861 tcacgttgag cgtgccatca gggtttttcg caaacgtcgc cgtcagggtg ccattccact
    34921 tcaaaagacc aaacggtccc gagcagttgt tgagcgtgat cgtcacgatg ggctgcgtcg
    34981 gatccttctg acgggtcgca cagcctgccg gccaccactg cgctgccgca agtgcgctcg
    35041 ctgccgtcgc cgcatccgcc gagttgattg ggctggtcag catgtattcg accgcgcccg
    35101 actgcgacat cgcagcgctc gcctcgccac catcatcgtc gtttgctgcc gcaccaccac
    35161 aggcgacaaa gccagtggcc agacacagcg aaacagccag aagcgatccg aaaaagcgct
    35221 tgtttgacat ggggagttct ccaggtgtgg gttttttcat cggcgcgtcg cgtcgcaaaa
    35281 accggccttt tggcctggct gcgggcgacc tgccttggga acgctcacct agacgatgtg
    35341 ccctgcgctg tggttccggc ccgcggcaag atttttgtcg agatcgacag cggcgatgtc
    35401 agtgccgcag gtggatcgcg cagctgccgc cttcgcactc gccgcgcgcc accgcccgtc
    35461 cccagatcgc atagcgatag cgcgccacga gccgataacc caagtcgcct agctgacgca
    35521 gacccggcac gtaatacagc ttggccagcc agcccaagat gggccgatgg cggagcgcga
    35581 ccaccaccgc ttgcagtcca gaaaagcgtc ccagggtggt gacaagctgc attgccgcca
    35641 tcgcttcgtc gtaggtgatg cccagcctat cgagcagccc ttcttcctgg agcggatgcg
    35701 gttcgatctg gggccctgcc atgcgcacaa gccgctgcgc ctgggcgcga cagaacttgc
    35761 agtctccatc gaagatcagc agatcgcgga cagcctcgct catcgtcttt tgttccccca
    35821 gcttctttca cgctatggct gggcgctttt tttcggcggc tcggccagca cgtcaaatgg
    35881 gatgtcgtcg agcggatagc cttgccagcg cgcaaagtca aagtgccacc actccgtcgg
    35941 catgccgaca aagccggcct ttgccatcgc ttgttccagc gtggtgcggt tttggatcgc
    36001 aaggggcggc agctgctgat agctgcgatg ggctttttcg ccaaagtcat caaacggcgt
    36061 cggcatcaca agctcttgcc cttgtgcatc gaccagcgtc aggtccaccg ccgcgccgcg
    36121 gttgtggcgc gagcctttgt ggggatttgc gacatagcgt tcgtcgtgga cgatgtccca
    36181 tagctgatac tgcacccggt ggggccgata gcagtcccac agcttgagcc gcagcccggc
    36241 tttgcccaga tcgcttgcca ccagcgccag cgcctgcgcc acatcggccc gcaggtagca
    36301 gcgcgccgac ttgggataca gcacctggcc ggtaaagttt tgcgtcgtcg catagcgcag
    36361 atcgaccacc acggaaggtg ccacctgcat cacgtcgaca agcggcggcg cggtcttggc
    36421 tgttgccgcc tgtcccgttg cacagcctac cccgaaaagg cacagtccaa gccacctgcg
    36481 tcgcatcgct acctcgtctg gctacagctc gtgcgggcct tccgcgtggc cggtgatcag
    36541 cgtgcgcagc gtcgccatgt cgtttgtccc agccaacagc acgatttggg tgcccggcga
    36601 aagcaccacg tccgcggtcg ggttgtagat gaactgatcg gactcgggcg gcttgatcgc
    36661 gacgaccagc gcgctggtct tgccccgcag gctggcgtct tgcagccgct tgccggccac
    36721 cgccgacttt tcacccacct ggagctgctc gaaccgatgc gtcgatcgcc ggtcgcgcat
    36781 catgttgtca aggaacgtca ccaccgtggg gcgaaccagc tcgctgacca gccgcagacc
    36841 acctatgtgc gtcgggttga cggtgccgtt tgcgccagca atggccagct tgcgacggct
    36901 gttgtcttcc agcactttgc tgatgatgcg caggttgcgg ttcatctgcc gggccgtcag
    36961 cgtcacaaag acgttgtcct tgtcttcggt caggcaggca atcaggccgg cggcgcgctc
    37021 gatccccgcc atcatcagga tttcatcgga ctccgccgat ccaacgatgt aggggaactc
    37081 gcccagctcc tcgtttaagt gacggagctt ggtttcgttg tggtcgatgg cgacgatctg
    37141 gcgtccggtc gacataagct cggtgacgca gtgaagcccg gtcgatccga cgccgcagat
    37201 gatgatgtgg tccctaagcg cggcgatctt cttttgcatc cgtcgtctcc gaaatacgtc
    37261 tgtgaacagc ccctcgacga ccagtgcggt gacgtttgac accaaaaacg ccggcaccgc
    37321 aatcccgacc agggccaaaa acatcgtgaa gccttcggcc aggtgcaggt ttttcagttc
    37381 cagccagtcg ccatagccga tggtcgtcag cgtgatcacc accatgaaca ggcagttcat
    37441 cggcgtccag tcggcccgtc cgtagtgctt gcccaaaagc cagtacaccc ctgtcgacaa
    37501 gagcgtcagc agcaaaagca gcagggcgcc ccacttcatg aagcggaagg ggtcgtagcg
    37561 cggttcctca aaatgaggca ctgggctatc ggcagagctc acggtacggc tatcgtacca
    37621 acatttttca gtttcgcccg tgattggacc tggtttggac ctggccgcgg acctgtgctg
    37681 ctgggccaat cggcggtcca gaccaggcgg cggacctgtg ctgcagggca gcgcggtggt
    37741 cgagacctcc gcgaaacgct gaaaacaggc cgaagcggag gtcaagagca gcccaaagac
    37801 ctgttgtgca gggcaaaggg gcggtctaga ccgctgcgaa acagggcaaa cagggtcaag
    37861 cgaaggtcga gaccgcggcc aaacactgcg aacagggcaa acggaaggtc cagaccagcg
    37921 cgcctggctg tgcagcaggg actggggttg gccaagaccg gctggggcgg ctgaaacaga
    37981 aaaattccgt cgggatgcga aaaaaaatgc gggggcaccc tttacggaaa cgtgcgccta
    38041 aggaatacat tctgccgttt gtccatgcgg tttggacatc tggaggccaa atgaatcctg
    38101 atcttgattt cgatccgtcc cagtacaccc gtcttccccc gtacctcgac aacgcgacca
    38161 cggtggcgct cggtcggcag cttgtcgccg ctgcgtcggg catcgtcgca cccgcggtca
    38221 aaaagagcac gtcggtggtg tcgcgggcga cccagaagct ggccgatggc atggccgaac
    38281 tggtcgggct ggatgcgccg cagcagaagc gtcccatcga ccaggctgcc gaccatagct
    38341 gggcggggct tcagcttcgg ctgcttgggt ggttggagct gccggcggcg gatcatcccg
    38401 aagtcgccga ggcgcaggcg ctgcacgcca agctgttccc caatgggctg cggtttaccc
    38461 agctcgaata cggggcgcag tgggccgaag ccgaagcgcg gctgtcgtgg ctcaaagggg
    38521 cagggcagct tggtgtgctg gagcgcctgt gcggcaagcc gtttgtcgcc gagcttttgc
    38581 gggcgcacgc cgcctacgcg gaaatggtcg gcaccgatgc caaaaagcgc gcaaccctgg
    38641 ccagccgtcc cgacctgacc cagcttcgca aggtggccca aaaagcgatg ttggcccatc
    38701 agattcagct cgtggcgctg ctgatgtctg gggaagcggt acagcaggcc gaaaaagcgc
    38761 tgcgccccct cgatgactac cgcgaaaagc tgatgccaca aaagcccgcc aaaaaagaag
    38821 acccaagccc cgcccccgaa accccagatc ccgccccggc tggggcgtaa tgttgccact
    38881 ggagcgtcga gcgactcgga tgctcgtcag caggttccga ttcaggaaag gtagattcca
    38941 tgggtgactg ctccgccgcc aagacgggga cttcggcggg ccgggcggtg gttatgtggg
    39001 ggggcgtttg ggcggatgct cgcggcgctg gttttcgacc cactgccagg cttcggcttc
    39061 gctcgaaacg aacgtgttgt gcagcggcga gctggtgata agcgcggtgg cccgctcgat
    39121 aaggcggatg atcgtccgca gcagcgtgtt gttcgtcacg acgacgacat agagccgcgt
    39181 tacatcgact cgcttgccgc gtgcggccaa aaacctgcgc acgtccgcgg tcggaagctg
    39241 gacatcgcgg gcgatgcaca gggtcagcac ccgctcgtaa tgcgcaaaca gcgcctcttg
    39301 gggaagtagc agcgcggtga tctcatcgag cgtcaccagc ccgctgagct ccatgacgaa
    39361 cagatcgccg tcgacgtaat agcgctgcgc tccgacagac tgccaagctg gcatggctgc
    39421 atttcagttt gcctgaaatg ccgcaggatc gagcgcgaca tcgcttggga aaacgagagg
    39481 gcccagacaa acgtgtctgg gggtgagggc tggtgatgga ggagagggga gagggcgggc
    39541 cgagcgctac tttttgccga gcgcttcaag ctgcgcgagc gcaaagtcgc gatcagcttc
    39601 cgccgcgccg cgctccgctt tttcgccctt gagcttctcg ttggcatcgg cgagcagctt
    39661 ttctgctgct tccgccgaca gcttcgcttg tggcagcgcc cgctgaacca gcgcgaccac
    39721 cttgttctga ccgctgactt cggcaaagcc ggggccgacg gccagcacgc cgcggtcctg
    39781 gcccttgcgg tagcgcacag tgccggcctt taccgccgac agcatcggaa cgtgaccagg
    39841 cagcacgccg aactcaccaa gcagtccggg cagcgaaacc tcgtccacct cttcggtgag
    39901 ggtctttccc tcgggggtca ccacatgcag cgagagcatg gcttacttcg cctcggcgag
    39961 gagcttcttg ttcttttcga ccgcttcttc gatcgagccg accatgtaga acgcctgctc
    40021 ggacaggtga tcgtactcgc cgtcgacgat gccgcggaag ctgcgaatgg tgtccgcgag
    40081 cggaaccagc ttgccgggga tgttcgtgaa ctgctcggcg acgtggaacg gctgcgacag
    40141 gaacttttgg atcttgcgag cacgggcgac ggtctgcttg tcctcttcgg aaagctcgtc
    40201 cataccgaga atcgcgatga tgtcctgaag ttccttatag cgctggagca cgcgctgcac
    40261 gtcacgcgcg cagcggtagt ggtcttcacc gacgacgtgc ggctgaagca gcgtcgaagt
    40321 cgagtcgagc gggtcgactg ccgggtagat gccaagctcc gaaatcgcac gcgacagaac
    40381 cgtggtcgca tcgaggtggg caaaggcggt cgccggcgcg gggtcggtca ggtcgtctgc
    40441 cggaacgtag atggcttgga ccgacgtaat cgatcccttg tccgtcgacg tgatgcgttc
    40501 ctgaagggcg cccatttcgg tcgacagcgt cggctggtaa ccgactgcgc tggggatacg
    40561 accaagcagt gccgacactt ccgaaccggc ctgggtgaag cggaagatgt tgtcgacgaa
    40621 gaacagcacg tcgcggcctt cgacatcgcg gaagtattcc gcgacggtca gtccggtcag
    40681 tgcgacgcgg gcgcgggctc ccggcggctc gttcatctgg ccgtagatga gcgcaacctt
    40741 gctcttttgc aggtcgtttt cgacgatgat gccgccttcg ataaactcac ggtacaggtc
    40801 gttgccttcg cgggtacgct cgccgacgcc agcgaacacc gagaagccgc ctttggcctt
    40861 ggcgacgttg tggatgagtt ccatcagcag aacggtcttg ccgacgc
//
LOCUS       DAORGN010000017        39917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_346_length_39917_cov_7.086248, whole genome shotgun sequence.
ACCESSION   DAORGN010000017 DAORGN010000000
VERSION     DAORGN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 39917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39917
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_39917_cov_7.086248"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="PKL17_03565"
     CDS             complement(<1..1856)
                     /locus_tag="PKL17_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK43834.1"
                     /translation="MSRPLVSTAAICLWGTASPLFAQAPQNPTAKDSATTQSLPAAKS
                     AEAPVTPAASATPPAETLVPGATAAPATASAPNPTSAPSPTSAPNPAQTPNAAAVSTP
                     AQTPAPTQTPEQAAATQAPTAAAPPAAEATPEPGIDKRPIEVSIIVGGHFFSSTAAIG
                     RSDYAAPGSELRHSVALGARIGYGLSRHFLLEGEFVAMPTQLVDKPAQVLVYTGRAHL
                     LFRWPFGKNDRFQPFILAGGGAIGVRPDPGLPLHSEVLGSFHAGAGMRFDLNRWVGLR
                     VDGRIVLLPETKTPSFTQDYEVLGSLYGRFGYGSDKPAEPAKPLDIDQDGVPDKEDRC
                     PTEKGAIENGGCPDRDTDSDGVIDRLDKCPAQAGPSENGGCPDTDSDSDGVVDRLDKC
                     PNEIGPQVNAGCPDTDSDSDGIVDRLDKCPQQPETVNNYQDQDGCPDELPANLARFVD
                     KKLEGVQFLPTKADLVPSSFAVLDQAVAALKEVPSVRLEVQGHTDNVGNPNDNLTLSQ
                     QRADSVKAYLIGKGVDPARLTAVGYGSTQPVADNKLPAGRAQNRRVEFKLLPPARPTV
                     IRKPQPAPITPIAPAPQSVTAPATPAKAPVIPPAATNPATQAPSVVPVAPPPV"
     gene            complement(2033..2926)
                     /locus_tag="PKL17_03570"
     CDS             complement(2033..2926)
                     /locus_tag="PKL17_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK43835.1"
                     /translation="MSSSRNCVTQELSTQYPRHVAALVCRGLVLCLLLFAQAAAASGD
                     GDGDEPAPAVADTKPAERKSPSAAPPVPVPTEAKANETAFALLGAPPQAEVRLDGKLI
                     GITPLPPRTAITPGPHRLSLSRRGYSTFSSDMTAIANRLVAVEVELTPLLAVLLVRSN
                     IQAAQVFVDEDLRGETPLELELRPGPHVIRVRRVGYIEETWSVSTVAGEQYEKELQLE
                     QQPEQKRREEAVFKTEKRWYSRWWVWTLAAVGASGVAAAIVTPIVLSRRSDCEKLGGE
                     VCFPISLQPTALQVGLTIPLR"
     gene            3348..3761
                     /locus_tag="PKL17_03575"
     CDS             3348..3761
                     /locus_tag="PKL17_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43836.1"
                     /translation="MARQSVLLGCLALSACGGHPIKVQMTIATDANNNSPVLISVVFP
                     KNQVLFKKLLDMNAKQWFSQREQLLRDYRRELDETYFEFIPGQQVPELTRKVPSNVPQ
                     GIMFVNYQTPGSHRYTFDTNQPLKIGFSQQSVNLQ"
     gene            3780..5120
                     /gene="tssK"
                     /locus_tag="PKL17_03580"
     CDS             3780..5120
                     /gene="tssK"
                     /locus_tag="PKL17_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNK43837.1"
                     /translation="MPSAHDLPPTIQWHEGMLLAPQHFQQLVQRTEKLLTYHVSLGLP
                     YQFGVIRLRYESTQLFTGTLRVTELEAVLPDGLVVSFKTGPSAELQVDLKSHTENAKH
                     TPIPVYLVVPIERSGGGKGDADLGRYLSVPGDEVDDEVSGGRPLRIPRLKPRLRLLAG
                     SEPPPPRFVALPLLAVVFKNGAFVPADYIPPLLRVEVDSSLGEMCAQVSSLIRAKATY
                     LLEVIRSPAITTKPTAIEENRRLLSQLLVNLPPFEALLATGTSHPYALYVALTAVAGS
                     VAGVGSQLMPPVFPAYDHLNPRRSFGEVVQFVNQSLSDGISEAFTALPFRNENGLFSL
                     QFEREWTRRTVVVGFKGAPGATDQQINEWVASSLIGTASRFRVLRERRLLGASRTAID
                     RVEGLVATRGMLLYQLQVNPDFIVPSEPLVIGNSVEKPPGMRPAEAYLYVANKE"
     gene            5174..6139
                     /locus_tag="PKL17_03585"
     CDS             5174..6139
                     /locus_tag="PKL17_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HNK43838.1"
                     /translation="MATETSEQLFLVDYFREFYAAVLQHRMQIEKASAPAVEEDSLEI
                     READILDEERVPGEEPSQIAPDMVQARLVELLDTQQQAVQRRGDPREQKRFSEIQYVM
                     AAMADEVFLSIEWIGKEYWSTHLLEERFFNTHDAGTRFFDNLDSLLQNRDATRADVLA
                     VYLLAVSLGFRGKHRSVSSAPQIAHYRNQAYVTLFQRSPALPESTPLFPQAYAHTLDG
                     QPPSWLPQLRPWLLSLVGVAVMYLLVAHVIWDRRSTSVSNRLEEILQRKDDSAASGSR
                     KVDSRSELPAQPDAPKTELRIPGSDVKTAAVTNEGTRGRSGTSAP"
     gene            6359..7309
                     /locus_tag="PKL17_03590"
     CDS             6359..7309
                     /locus_tag="PKL17_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43839.1"
                     /translation="MFLSKSYEGAIKQFTQAYRLDPKPQYLFNIGQSYRKLGLAAEAA
                     EYYQRYLHDEQKIDPTIYADLTQYIDQYYASQEKAGGRPKSTAEPPEPAPLLAAEEIA
                     DAEELIAQYVRDYRAGNQAVANEIMDQLKGVLQLRRDPILMYHIANGYDQVRRRRDAL
                     EYYRRFVAIETADPRLRRRSFERIAILTPAPPGQKYLWPSLALAIPGLAGVLTGVGLY
                     VQSDSNFQLFQNESDEQKKRALRDKGTGLSIGSTAAYAAGGALLAGAGVFAIVALKKG
                     IRQSSEDKGTEKKETQKRSTLTGALNPLPGGIGLSLGGNF"
     gene            7309..8751
                     /locus_tag="PKL17_03595"
     CDS             7309..8751
                     /locus_tag="PKL17_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNK43840.1"
                     /translation="MLKTTLSALLFASLSLLSGCTLTMSYNQCVENADCKNADGTTGF
                     CSSDQLCLPSTPLERLCTSTIPEIPSPNAVHIGALLDLRDNGGRPMEAHRYIALKLGV
                     KQINELVQLDGSRPLALDVCNVGVSPIDADNATKILIEQRNIVAIIGPNSAAAATNMI
                     GRIKAAGVPTISPSIMDSGIADAASQGLFYRLTPVEREQGLQLVRELTTLSGGKSTSQ
                     LGLLSVSDSYGENIRRKFTTEWQRKDPVNNKTDRFFSYKENDANLLAVTTKTLLDAKP
                     SFVVLIPGANSQQVIGGFADLPFDPKNPASFESTTQVLLSSSGRTGELIAMARSGDDR
                     MKNHLSRITGVAPLSFIDSQEGADFKSSFVATNPGLPLEQDYMVGYTYDALFVLGAAS
                     ASFKGASRPDQILSVLRRLNGTANSVSLTPRLFNETVQKLAQQPNDSFTLLGVTGAIR
                     FATDGGRDPVLLERWKIDATNGVYVNTPAM"
     gene            8776..12885
                     /locus_tag="PKL17_03600"
     CDS             8776..12885
                     /locus_tag="PKL17_03600"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HNK43841.1"
                     /translation="MLIRIFIAVLILAVLAAGVFLFLRYRRKQQEASPEAAPPPPKDP
                     ERAKRIAQLKASLTTLVNEELRQLDQNVGGANGRYGMPWCLVVGEKGSGQSTVLQNTG
                     LHIPFRGASDALAQAGNCRFWFYDKGVAIDLGTEIWQDEELFAHFCGLLKTARPKRPL
                     DSVLVVLPATSLVGEDKRNPEQLKESSEELYQRLLKLQQNLTLRLPTYVIVSKGDTIP
                     GFTAFCGGLSGNQREQLAGWSSPYPINVPYDPSWVDQAFAELYRTQCGLQIDLLASAR
                     LSDKEQDAAFLLPRNTQALREPVKLVLDQLFRVNVYSESNLLRGLYLCGDATIASAMV
                     RQNPEEEGQKKPVFVHDLFGKKLFPESGLARPLKRGLLDQGRALRLAKAGLAASIVVS
                     ILLLWSAYANLSKDVRAVMGFLDKVPVETTLSVAQDRDRFALQTQELLSAIGSVATSR
                     LLSVRLPSSWLSSLDDEVQEAMRRSFERVVLGGLHEGLLLKAKQVLGLEGDLTAQAPS
                     DEPPPAGEPGSGRTGSASSERLTSKTVQVLPLQSMPEYQELRELSKSYLEFVQNVDTY
                     NRLGSEHHKRFDTVVPLVKYVFGVELQPVFLRNAELYQEALANATSRSFDMALVNPKV
                     QARAKTICRALHRRLFIDNAVAIEIDEVLAHVSRLKAESEGGGADLPILTELRDVLVR
                     LDRDLSRTELQWMSKDSLDLGEPMSELLAALRHFSPDGKMSDELRQEWQASHQQLRRR
                     LLEVQLPQLGLIIQRDNEKHRLVMTQGVVDLKANLETFLALSFVQQNLEKPHAQNSEG
                     SYRVVWNDDLLRQATGFSEAYDTYVRDRLGQVYRHIREVVKSTALERLGQNMPALVAQ
                     ARREERVLRFSGDVNALQDELGNEVGDLKRVSTVLRQILETYDRLSLKAAHSELYQQL
                     QEDSREILRRIDLLPEREEMYRVSSKLASWRGERPPVLAAFDVEDADALSQYIKTQRN
                     RIKGWGRDYAKVPVSLLDGLSSARSDDPLVGKWRNIVNQLEYFERMTPGNSVKEMESF
                     FDTTLLTVTPENCLDKLPRQSGDNTDFFLQSKARVHESVRKRCLVFAAEKILELYEHL
                     ARRFNQDLAGKFPFTRNPQSLDDPDALPRDVRGFFQEYDEFIRQYDAYVGRRETLGGS
                     GEVGREVNAFLQSLREVRPFFAPLLGDRGEQARYQVSVEYRINKSNEINGQQIAEWSF
                     ATGDQKVEDGQGFWQIGDRVKLMMRWAKDGPYVPAPTGQPRGASVDSNDAIAFDFAGI
                     WSLVRIIRMFRASQTDLRKSLDRQPHVLKFMVDIAERKRSGRVRLLPDSYYTNKGSTP
                     TGAAKMAFLYNKAVVFVRMVLNPAESKELLVIPVDWPTLAPLTRARSGQ"
     gene            12895..13998
                     /gene="tssA"
                     /locus_tag="PKL17_03605"
     CDS             12895..13998
                     /gene="tssA"
                     /locus_tag="PKL17_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNK43842.1"
                     /translation="MSEKPPIDIESLLAPISEREPAGEWLRYEGTYDAISEARREDDE
                     SLPQGIWQTKLKRADFAKVSQLGQKALRERSKDLQIAVWLCEAWLKLHGIPGLCLGID
                     LCAGLLSRFWPTLYPLIEDGDASYRLAPIEFLSQKVALRLKEIPLTESVSERPPLTWN
                     DWERALRNEKAGAGSDEILPAQFLASASATPSALFAERFAELEALQDCAKRLEAVVLG
                     FEEEQPSVLLSLRNLVTDMRALIGQYAPAAPIAAAHSPDTVPVSGSQDGTSPKTAENR
                     AHGLEASVQPGSEMREESSTGPIRSRAQAYQRLVEAADYLQRTEPHSPVPYLVKRAVA
                     WGNMDLIQLLAELVAGDADRHVIYALLGVKAPH"
     gene            14099..14605
                     /gene="tssB"
                     /locus_tag="PKL17_03610"
     CDS             14099..14605
                     /gene="tssB"
                     /locus_tag="PKL17_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010430713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNK43843.1"
                     /translation="MSESTQHKLDRVRPPRVQITYDVEIGGAIQMKELPFVVGILADL
                     AGQQDALPKLKDRKFTQIDRDNFNQVLANVAPKLKYSVPNQIQKEGKPVGVDLTFHHL
                     DDFHPTSIVKNVPQLRELLEMRQRLVDLLTKLDGNDDLQTQLAGLLADKDKFGAVKAL
                     AAKSAVSS"
     gene            14618..16084
                     /gene="tssC"
                     /locus_tag="PKL17_03615"
     CDS             14618..16084
                     /gene="tssC"
                     /locus_tag="PKL17_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNK43844.1"
                     /translation="MAENETTTKTTTNILDDLIRDGKMARDENQLAHSRTLIGEFVNT
                     VDPTAVAPTVDLAKVILEKIAQIDKMLTAQVNSIMHDERFQKLEGSWRGLNYLVMNTE
                     TSTSLKLRVMHATKKELLSDLEKASEFDQSNLFKKVYEEEFGTFGGAPYGCLVGDYEF
                     GRHPQDISLLGKISNVAAAAHAPFISAASPKLFDMESFTELGNPRDLAKLFESSELIT
                     WRSFRESEDSRYVALTLPRVLGRLPYGNKTVPVEEFQFEEDTDGKDHSKYLWSNAAYV
                     MGQRITNAFALHKWTAAIRGVEGGGIVQGLPAHTFKTDEGDIALKCPTEIAITDRREK
                     ELSDLGFVSLCHCKDTDYAAFFGGQTTNKPKVYNTPQANANASLSSQLPYILASSRFA
                     HYLKAIMRDKIGSFLTRENVQNYLNNWMSSYILLRDDAGQETKAKYPLREARVDVFDV
                     PGKPGVYRAVAFLKPHFQLNELTASIRLVAELPPPAAG"
     gene            16147..16635
                     /locus_tag="PKL17_03620"
     CDS             16147..16635
                     /locus_tag="PKL17_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNK43845.1"
                     /translation="MQNIFWEIEGIKGESTSAQGKDMIELLSFNHGVAMPLTHGPSNT
                     SRASGRCVHQDFTVTKHVDLASPTLNLKCCGGEDIKSMKVHIWKADSAGKPIEYITYT
                     FDSCILTSVSVGGGGDQPIETLTFSYKKITWDYAQQLQKQPGGNKGKSSSNWSLEENK
                     GS"
     gene            16673..17152
                     /gene="tssE"
                     /locus_tag="PKL17_03625"
     CDS             16673..17152
                     /gene="tssE"
                     /locus_tag="PKL17_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNK43846.1"
                     /translation="MPLPSKKHVAAVPLFERLSDRKPEQPREPVPLRTLSYPELLDSV
                     RGEVTRLLNTRSGLASHELLERERSVIDFGATDLLWVNPFDPESMRHLTRLLVQTIEK
                     FEPRLAHPRVTIVQFVQTESRLILQVSGTLVMEQLREPVSFRVTASPRLLEHRSDEQ"
     gene            17142..18950
                     /gene="tssF"
                     /locus_tag="PKL17_03630"
     CDS             17142..18950
                     /gene="tssF"
                     /locus_tag="PKL17_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNK43847.1"
                     /translation="MSSDAKDELLRAYQRELAYLRGMGAEFARKYPKIAARLDLSAET
                     CADPHVERLIESFAFLTARLQSRLDADFPLVTTSILECLYPHLLQTIPSMSIAHFDVD
                     PEQGLPLGGFTIGKGAKLFAQTDTGALCRFRTGADVTLWPISIVSAQLESTDRYPYLD
                     AYPKGIAMLRLKLECQTDNFGELDLRSIRVFVNSSAEVASAIYDLLVASTVGVVLLPD
                     GRPEVQFLGPSALRPVGLEKEDSLLPSPEYAHPAYRLLQEYFVFPQKFLFFDIDLPRI
                     QLARKTADVLILLSEAPPRSVAVTKSTFMLGCAPIINLFPKTAEPIRLTHRLPEYPLV
                     ADKREEKTCEIHSIDKVSQSADPNSSQNIAPFFSFNHPASGEQHQAFYYAQRRPTGRA
                     DLGGLDTYLSFVDMRFKPTQPATQTVFAHTLCTNRDLAAQMPARARLQMEDAAPVKAV
                     TCLSKPTEPITPPLGGAALWQLVSHLSLNHLSLIGGRESRDALREILRLYRMSPTTAT
                     ENQILGITELQCIPKTLQAGRDAWRGFVHGQEVQITFDEGLYVGHSALMMSAVLSRFF
                     GLYTAVNSFTMLVVRSRQRQEAWKKWPPLAGARNLL"
     gene            18914..20119
                     /gene="tssG"
                     /locus_tag="PKL17_03635"
     CDS             18914..20119
                     /gene="tssG"
                     /locus_tag="PKL17_03635"
                     /inference="COORDINATES: protein motif:HMM:NF018675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNK43848.1"
                     /translation="MATAGGRPQPSVIEELLSEPQRFNFYQLVRLLEMMEPDSVSVAE
                     GGDPTAEAVRFRSTFSLAFPPSDVVAIENVKTESIEDEFEQLIRRANGEEKTPTVTVS
                     MLGLGGAHGPLPHSMTEWVLERSQARDFSLRDFLDIFNHRLVSLLFRVRRSCRLGTEW
                     TSPDEHHFSRFLYGLMGMLTPGLQDRLKIPDRALLRYSGLLTKQPRDAAGLETLLSDH
                     FQVPVICHQLRGAMRPIEEDQWTKIGLSGQNQMLGGQALLGTTIWDQQAGIELEVGPL
                     PWNRYLDFLPGKSGLTALVAIARYYVGPEFDLFVSLVLKSEDIPRARLAYPSKETESA
                     TAPAGVTAPAKGALAAKAASSQGQLATTAPVLGLTSFLTHNSKLRGVPKVALGVVQRY
                     SDEPLRASP"
     gene            20116..22152
                     /gene="tssI"
                     /locus_tag="PKL17_03640"
     CDS             20116..22152
                     /gene="tssI"
                     /locus_tag="PKL17_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNK43849.1"
                     /translation="MSEHDFLSASTPAADALVLESFDGQEELSLPFLYSLQFLSKDRK
                     LDFKKMMGKAVTITLVLPDKSKRYICGLVTRFVESGYDPRHTIYIAELRPWLWLLTLH
                     ADNRIFQNKSVPEIIEEVFSDLGFKDFKKSLKATYAKREYCVQYQESSFDFVCRLMED
                     EGIFYYFEHSDGKHTMVLADDSDGYCKCTGSTDVGMGLDDTPNNLRNCTFEESLVTTG
                     YALTDYNFEIPSTDLYVKTGGGSPALVVYDHPGNYAKKNDGEKLAKLRIESVENGQKV
                     LRASSNCRGFTSGGKFKLTKHDRSDLNQEYVVRSVHHTADRANYRNSIEALPASVVFR
                     PLRRTRRQLAVGSQTATVVGKSGEEIWTDKYGRVKVHFPWDRKGKKDENSSCWVRVTQ
                     HWAGKGYGTFFLPRIGQEVVITFIDGNPDRPLITGAVYNAEQTVPYALPDNQTKSTTK
                     SRTSKGGGGFNEIRLEDKKDSEEIYVHAQKDMNIEVKHDRTAKIDNDCKTEIKNDRSV
                     KVDNNDSLKVKKNREVEVSEGDESLTVKKGNRTITVDKGDEKHTVKGKRDVTVHKDEK
                     HESKAKFQHDVTKDYILNVKGNLTITVDGDIKIVSKKGIKAEATAAMDFKSGADMKLK
                     AGANLKQEAAVNMDIKAGVGMKIEGVNVDVKAAAMAKLEGGAMAAIKGALLKLN"
     gene            22200..23354
                     /locus_tag="PKL17_03645"
     CDS             22200..23354
                     /locus_tag="PKL17_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43850.1"
                     /translation="MSDAETSASANPPAPTELVEHDEEGRVTMQGPLHEGKKHGEFCF
                     FAAGKLTQKANFKNDLLDGETVDYFPNGKVSQRLHFAAGKMHGLLRRFDDQGRPELIA
                     AYQAGELHGDLLTYENGQMVSQQHYVKGKKHGVGIIYGPDGQPTLSANHQNDKRHGEA
                     VYFGAHGVVIRREPYQEGLIHGLVQEFLPDGRLRETCQYQAGKPHGEQLVYYPSGKLQ
                     KRARYEQGAPSGNPELFDEKGRPLKPAETPDSPSMEAKAAEAAKQSALRGLMDVARKR
                     IVESAAPAVIAAIKAPEIPEVPELPAAPEIPSAPELPQAPEIPQAPEVPEELKLKPPV
                     AAEKAALAQKELPKPGKKAPPPPPPRKGLILEPPNIGTLPAEKTNLPRGK"
     gene            23354..23740
                     /locus_tag="PKL17_03650"
     CDS             23354..23740
                     /locus_tag="PKL17_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HNK43851.1"
                     /translation="MPNAVCMGAMLKCSFGVAPSSLVVLPTNLVMGPQPVANIMDGKP
                     MVNILPFGMCQSMANPMVAAATAAAMGVLTPMPCIPNTPAPWVVGAPTVMLGNMPILN
                     NSAKLMCLWAGVIEILPPGIPTVITP"
     gene            23749..24681
                     /locus_tag="PKL17_03655"
     CDS             23749..24681
                     /locus_tag="PKL17_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43852.1"
                     /translation="MATRVRKPDAAKTAEPAAEPAATTRRRATQSRTAATRRTASSSR
                     SQRIVLPSDLAEAEKEATTPSVADSAPALEVAESHQIAPVAKEEIAVSPTPIASPDAV
                     ADAPFSKKTSAKSVSEKKTKESHSEVRSESKDAGVSTETSSVLQRLSELQKLVTDGQD
                     RSLAELQKTQTQMVRNLSGLSRDVDKLLKHVQELQSRLAQSELVLSRVESTLGPLSRG
                     VSSLVMPAKPLERPLSPDVFEKSLGLALAPILAELVELRAQRARDSSLAQVEQQILMT
                     ELRRLRNVGLSQIERNTDPLSSVGKTGRRTPSKL"
     gene            24758..28774
                     /locus_tag="PKL17_03660"
     CDS             24758..28774
                     /locus_tag="PKL17_03660"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNK43853.1"
                     /translation="MSGKGTQRRWTCHLLALLALLVSSCSVPDGLMVTLRAPDGVRLQ
                     SYVIKVQDRESRKVVFLSGIQTISDKRTLATDPLKVALPFSQHGKFLLQVLAANVANV
                     ESLPQKGNSEPQYFFARIIDVGAYQDVEAKLLPVPPEFDVDGDHFPDAVTWTAQNPEA
                     AALYADQPLVLDCVDDDPPPGDLDPVRLRSFDIHPLAGRSCNVRLRPSHAPNAMPLPP
                     LQLFDATCSGEPRACEDKDGDGDPEGSDCDDNDKNRFHGNPRPRNCCECTDVTSCKTN
                     HNKRADMTLCQPKRCDTTFDYDCTGLSVDCFTDDDCDGYSPNNPDVKLRDCDDTDARV
                     HPNAQKLCDPPDGVIKDWACDGRPSAGCVPCDLDGDGFQRSEVLAGQTCPTINYKNSG
                     RPLDCDDNDRGVFPGALTYQGSAMKYGTLDASSRAFNVLSAMRGLCRATDPAGSPQNT
                     SCDGVASARQGCPTAACDADGDGFPKPLASCMVAGQPTDCNDTDPTIFPGAPLACDGK
                     DHNCDMMVDVCSFDRDKDGWDGATDCDDANADVHPFAPEMCNGRDDDCDGLIDEQNPD
                     SNGARLIQTGTDGSKRTTSCADSTVGLCGAKDSGGFYSGRCVCTSTLPSSTVDPKAVA
                     LCPAQDNSAGFTARCFGANQPKPQTCDALNPVDDDCNGSLDDATGAFLAEKGKTCGLT
                     VTGTRCKAGTVVGCVRSKENPFWALRVPGFAETDKYLVCTSQTDPIPEQCNGFDDDCS
                     GALPPNEQDLDGDKYMSCSGCQNTDNPALFNASMLYCNDCADNNRNVWPTIPPPSMQA
                     RDGAPELCDGLDNKCASGNVSMTPANDGFEQCGSGTDAGKGTCCPLLKLCIDPQTDFQ
                     HCGGCTGACSSTTADRCGGGQCMCNNEVACDPASQTKSLCKAGAGCVQCLSGTDCTRI
                     LGSGTLTKACMPAQNRCVECTDNSFCTNALFPGKQACDTVLGKCAVCLADTDCKAPAP
                     GCKVNPGDSSKNICVECTQNSHCTDSSKPTCDLTLNKCVPCLVAADCKATPSNCKTNA
                     DSTLNQCVECLSSNDCKTLTSKPICDTTKNKCVPCLADTDCPATASKCLKDAGGDNTK
                     NQCVSCLSGTDCSGVVGKPLCDLSNHQCVACKANMDCSAVVNNPACYVNAMDSTKNMC
                     VPCVLGSDCPMSTPTCKVNAADPSKNMCLVCLADANCTTTKPSCYKDPGMDDTKNYCV
                     ECQTNANCKSPTSLCYVDADPKKNACTVCLANADCKSPTATLCYVDATDKHLNVCTQC
                     LANADCNGANKFCQTVTGMPVKNLCVPCAANADCSGKTNKCYVDMTTPANTACVECLA
                     NMDCTSPKTCDMATHTCK"
     gene            complement(28823..29101)
                     /locus_tag="PKL17_03665"
     CDS             complement(28823..29101)
                     /locus_tag="PKL17_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43854.1"
                     /translation="MAQLSTCNKCKGFVRSDSKSCPHCDADKSSLSSWGLRALGLLGA
                     LGGGAFAVTLMACYGCPDPYCGAPPTDLGCTGADGGQGDGGTADGGCR"
     gene            29222..30445
                     /locus_tag="PKL17_03670"
     CDS             29222..30445
                     /locus_tag="PKL17_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK43855.1"
                     /translation="MSTRRLSVLSQIRPAYVVWELTLRCDQPCTHCGSRAGEPRESEL
                     SEAEALAVVPQLSALGCDEVVLIGGEAYLHPGFVAIIQALAAANIRPTMTTGGRGITA
                     ALATQMAQAGMASVSVSIDGTQRTHDLLRAVRGGFDAACQAVRHLRSAGIVTTANTNV
                     NRLNLGELEALYETLKELGIAAWQVQITTPLGRAADRPDLILQPYDLLDLLPRLALLK
                     QRGFSDGILMMPGNNLGYFGPEEALLRSPHPQGRDHFQGCLAGRFVMGIESDGGVKGC
                     PSLQSDSYVGGSLKQSPLSVLWNESPQLAFTRQRSTSELWGFCRDCDFAEVCMGGCSF
                     TAHALFGRIGNNPYCHYRARTLAKQGLRERLILRKSAPGLPFDHALFDLVVEPLTRPE
                     PVLPQTQLVKIRRPA"
     gene            complement(30450..31679)
                     /locus_tag="PKL17_03675"
     CDS             complement(30450..31679)
                     /locus_tag="PKL17_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43856.1"
                     /translation="MCDTLVVVKPDCVLFAKNSDRPSGEAQVLDWQPAAFHPPGSFVT
                     CTHVRIPQVRHTNAVLLSRPSWMWGAEMATNEHGVTVGNEAVFTRHPVPLVGLTGMDL
                     LRLSVERADTAERAVTVILDLIAQFGQGGSADRHDQSFRYFSSFLVADRQRAFVLETA
                     GPHHAIEEVRGVRSISNGLTIPGFAEQHSDRLKTWASRCRLRRPRTEQKAQDATQPID
                     LTAVLRDHGSVARARGTIDYHPLLGGMSGPCMHAGGLAVDSQTTASWISKLAQTGDSH
                     FATATAAPCTSLFKPVSVFAPLPASFLGRPQHGQADSHSLFWQHERLHRMVMQHPDRL
                     LSLFQAERDALEADWFTSLPPSEQAFSVAKQQLAKWTQAVADSLREHPVQTPQRPRLV
                     SHFWQRRNRQVGLSFDP"
     gene            31730..32833
                     /gene="asd"
                     /locus_tag="PKL17_03680"
     CDS             31730..32833
                     /gene="asd"
                     /locus_tag="PKL17_03680"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNK43857.1"
                     /translation="MNQKLRAMVLGATGVAGQQFVAALAGHPWFSLARLCASERSAGR
                     SYKDALRDSRGSLRFYAGGEPPAESLDLPVFDSKEVSTDGIDLVFSAVESDAAKELEP
                     RFAAHVPVFSTASAFRYEPDVPILLPGVNRSHAALLRTQQKRRGFRGFISPGPNCTTV
                     GLAITLAPIHAQFGVRRVVMTSLQAVSGAGRSPGVVGLDIIDNVIPFIAGEEEKVEKE
                     TRKILGHCGGASAESHAIEPSPIAVSCTCTRVAVLDGHTEAVFVETERPAPLVEVKAA
                     LLDFGRDLAALKLPTMPAQLISVTDDPYRPQPRLDRDCGGGLTTVVGRLREERALGAH
                     GVKYVLLSHNTKMGAAQGAILTAEQLLADGYFG"
     gene            33141..34454
                     /locus_tag="PKL17_03685"
     CDS             33141..34454
                     /locus_tag="PKL17_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43858.1"
                     /translation="MRKQQKKSLFLGAAASLALLGACNDYLTGQDGEPTGPLKVLRLT
                     LIDFSANTGHLVFTDTSVPSDCTLPQYKDLKQCTNDPFMDTYGVKKSPPNPDSLRRLR
                     VVFNKAPLLDLDATNRGFNTTELELVPDSGVPTDLKLVDQKVISLACTNCSTDPTLGA
                     GIPLTYNSLQLTGSDLSPDPSQFPYGPGLQMEVLTSCTSTTNKFKCDKIGLTKTTVDP
                     LAALEPDASYTVQLSKGLSGRDKNPVLMDDRAMALLSFRTEPFQTNSVGNGDGASDAE
                     VRPGMSGSGTASDPYMVADSLVALDGAISIALNAAVDPSRLNGTSATATVSVNGGAAT
                     PVPLVVSPNAGGDCGNLRSIYVAPAGGAAWATPSKPDTDDVVVTITLKGAAIYDLSQI
                     KGHPLGKGIHTMGKDIVIRAKLLKGATPMDYAGVPSSAVEDPNNC"
     gene            34479..37832
                     /locus_tag="PKL17_03690"
     CDS             34479..37832
                     /locus_tag="PKL17_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK43859.1"
                     /translation="MRRKKSKTPGKAAVVLRAKLLPWMLVLPGAYLAPQVALAQQTLA
                     QIQGTVKDENGKPLAGVTVVVQSPALQGEQAEITDNNGRYIITQLPSGDDYKVSFFFG
                     ADDKPRVERPGIRLSLGKTVTVNAEINLSSTKREVKVIRESAPVVDTASAATGVEVNQ
                     EVLRATPVRGRTFESVLTLAPGTSDVAGKGGAAGGDVGVQISGSTGNENNYIIDGLNT
                     SDPNLGLIGTELSQYFIQEINVITGGYQAEYGRATGGVVNIVTKSGGNEFKGSVFGSF
                     QPFQLRPQGVARLGEALITRGRTNLLYDFGFELGGPIVKDRIWFYLGFAPTFTENVTQ
                     RQARALSFDMTTGKAAIDPDFQCPTYLSNTLLCDGPRTLALRTDEINYAQEVNSINRL
                     FNGIAKLQFNLNPDHNIQLTYIASPRTGNFYGATRAVDLSSLRASNTDQVHDGIFHYI
                     GKLFDRKLQLDVLYGYHYQGSRTDPEQPTIPSISWAAAQTDPFTLADFEDVGACRRQM
                     RTDAMGRSVLFNPCPLTGYARGYGQYSESLLQRHQLIASATYFLHLTSAWNPFRGIHA
                     IKVGFEFEYLDSYNNRTYTGPDLDPNDPASGHRAYSQQPDGTIQISREYAKQNLETKQ
                     IEHLNNFVGRSYTQNFAAYLRDSWNIGWAPGLVLNAGVRWEGQELYGSDGKRYVDIKD
                     NWAPRIGAVYDFTQLTSRPGRSKVFFNYGRFYQSIPLDINDRQLTAEGMYSSGFVPGA
                     NCDKVTLQPGGRAVPVPSAGCQFLAADGGVVNGGRAPLIAPSIKGQYINEITFGVNYD
                     VGLDIVLGVSYIHRDLGNIVEDMSPDGGTTYLLANPGVAADPNVVAQLQSEVDRLKPA
                     GTAANAKVEDQLAYQDAVSLLGAYKLVGSVFPKARRNYDALVLTLNKRLSNRFSIISS
                     YTYSRTIGNYPGTFSSSNGQLDPNISSQFDLIDLLANRNGPLPTDRPHNFKATAFYDQ
                     PLFGDKGKLTIGLTFTATSGRPIEVLGRHPYYGRREVYILPRGSGGRTPMVTQFDLHV
                     GYDHKLTQTVGMNLFIDVVNLFNQQEVTNVDDEFTATTVSPIVNGQLTDLKHLKANNG
                     TLPTFNSNYGQPTSYQLPLYMRFGWRLTF"
     gene            37953..38369
                     /locus_tag="PKL17_03695"
     CDS             37953..38369
                     /locus_tag="PKL17_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNK43860.1"
                     /translation="MKIVRIDHVALCVPELDPALAAWQSVLPLLLGPREYVPTQLTEA
                     AFLLTPAEADACVELIAPKPRGENAGLEKFLEKRGGLHHIAFAVEDIAGALVELAERG
                     VPLIDKVPRPGARGHQVAFLHPKALGGVLVELVSAH"
     gene            complement(38459..>39917)
                     /locus_tag="PKL17_03700"
     CDS             complement(38459..>39917)
                     /locus_tag="PKL17_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNK43861.1"
                     /translation="RRKQLDPRLCSPTVLEHDPLSREEVAEHLDAIMPEDTALLIYTS
                     GTTGPPKGAMVSHRNILSMFQAGDYFTDFFADDVTMSFLPMAHSAERVLSCYGRLASG
                     VATAYASSMSRLLDELSEVSPTVFGSVPRIFEKAYGKIQSELEKRPLAVRKLFAWARE
                     TSIARMRLLHRGEKVPLPIQVAHELADRLLWSKIRGFFGGRVRYFIVGAAPTPKDVLE
                     FFWAAGLPLLEVYGMTEATTVTHFNTLQNFKLGTVGKLIPGLEQRIADDGEILLRGPW
                     VFKGYFRNPEATASTIVDGWLHTGDIGSLDEDGYLRITDRKKHLIITAGGKNLSPGNI
                     ESAIRGTDPLISHILAHGDRRSFVSAIIAPSPLETLDFGLRRGLVSESEVKARSRELI
                     ENPSARSDQLERAMAPVVAHPDFHARILSAVRAGNQRLAHVEQVRKLLLLPRDLSQEH
                     GEMTPTMKVRRREVEQKYAAQLDRLYQDDSFGLSV"
BASE COUNT         8341 a        11356 c        12195 g         8025 t
ORIGIN      
        1 acaggcggcg gcgccacggg cacgaccgat ggggcttgcg tcgctgggtt ggtcgcagcg
       61 ggcggaatca ctggcgcctt ggcgggggtt gccggggcgg tgactgattg cggcgctggt
      121 gcgatcgggg tgatcggggc cggctgcggt ttgcgaatga ccgttggacg cgccggcggc
      181 aaaagcttga attcgacgcg gcggttttgc gcccggcctg ccggcagttt gttgtcggcc
      241 accggctggg ttgacccata acccacggcg gtcaggcgag ccggatcaac acccttgccg
      301 atcaggtacg cctttaccga gtcggcccgc tgctgcgaaa gcgtcaggtt gtcgttcggg
      361 ttgccgacgt tgtcggtgtg gccctgaact tccagacgca cggacgggac ttcttttagc
      421 gcagcgaccg cttgatcgag cacagcaaac gacgacggaa ccagatcggc tttcgtcggc
      481 aggaactgca cgccttcgag cttcttgtcg acaaagcgcg ccaggttcgc cggcagttcg
      541 tcggggcagc cgtcttgatc ttggtagttg ttgaccgttt cgggctgctg cggacacttg
      601 tcgagccgat cgacgatgcc atcgctgtcg ctgtcggtat ctgggcagcc ggcgttgacc
      661 tgcggaccga tttcgttcgg acacttgtcg agccgatcga cgacgccatc gctgtcgctg
      721 tcggtatccg ggcaaccacc gttttcgctt gggcccgctt gcgcagggca cttgtcgagc
      781 cgatcgatca cgccatcgct gtcggtgtca cgatcggggc agccgccgtt ttcgatcgcg
      841 cctttttccg tcggacaacg atcttcctta tcaggcacac cgtcttgatc gatatcgagc
      901 ggcttggccg gttccgccgg tttgtccgat ccatatccga accgtccgta caggctgccg
      961 agcacttcat aatcttgggt aaagctcggt gtcttggttt cgggcaaaag cacgatccgt
     1021 ccgtcgacgc gcaacccaac ccagcggttt aggtcaaagc gcataccggc gccggcgtgg
     1081 aacgatccga gcacttcgct gtgcagcggc agacccggat cgggcctcac cccgatggca
     1141 ccaccaccag cgaggatgaa cggctggaag cgatcgtttt tgccaaacgg ccagcggaac
     1201 aaaaggtgcg cgcgcccggt atagaccagg acctgggccg gcttgtcgac aagctgcgtc
     1261 ggcatcgcga cgaactcgcc ttccagcaag aagtggcggc ttaggccgta gccgatgcgg
     1321 gcaccgagcg cgacgctgtg ccgtagctca ctgcccggcg cggcgtagtc acttcggcca
     1381 atcgctgccg tcgacgaaaa gaagtgccca ccgacaatga tcgacacttc gatcgggcgt
     1441 ttgtcgatgc ctggttcggg cgtggcttcg gctgccggtg gggccgctgc agtcggggct
     1501 tgggtcgcgg ctgcctgctc gggcgtttgc gtcggtgcgg gcgtttgcgc cggtgtcgaa
     1561 accgccgcgg catttggcgt ttgcgccgga tttggagccg aagtcggact tggagccgaa
     1621 gtcggatttg gcgctgaagc cgtcgccggt gctgccgtcg cgccggggac tagcgtttcc
     1681 gcgggcggcg tcgcgctggc ggcaggtgtg accggggctt cagcgctctt tgccgcgggc
     1741 aaactttgcg tcgtggcgct gtcttttgcc gtcggatttt gcggcgcctg cgcaaaaagc
     1801 ggcgatgccg tcccccaaag acaaatcgct gccgtcgaca cgagtgggcg gctcatcgaa
     1861 aaccgccgct caccagaccg aaaccgaaga cttagctgac ctgcgcgtcg ccgcatgcac
     1921 gtaaactccg accaggcaaa aggaaagtgg gcgcatccta gcagccgact cggagtgtgt
     1981 aaacgcgcac gtagcggcgg gctgtgatag gcggggtctt tttgcggagc ggttagcgca
     2041 gcggaatggt cagaccgact tgcagggccg tcggttgcag ggaaatcggg aagcagactt
     2101 caccaccgag cttttcacag tcggaccggc gcgacagcac aataggggtg acaatcgctg
     2161 ccgcaacgcc gctggcacca acggcagcca gggtccacac ccaccagcgc gaataccagc
     2221 gcttttcggt cttaaagacc gcttcttcgc ggcgcttttg ctctggctgt tgttcgagct
     2281 gcagctcttt ttcgtactgt tcgcccgcaa cggtcgacac cgaccacgtc tcctcgatgt
     2341 agccgacgcg gcggacgcga atcacatgcg gaccggggcg cagctccagt tccagcgggg
     2401 tttcgccgcg gaggtcttcg tcgacaaaca cctgcgcagc ttgaatgttc gaccgcacca
     2461 aaagcaccgc caaaaggggc gttagctcga cctcaacagc gacgagccga ttggcgatgg
     2521 cggtcatgtc agacgaaaac gtcgaatagc cgcgtcgcga aagcgagagc cgatgcggtc
     2581 cgggcgtgat cgcggtgcgc ggaggcagcg gggtgatgcc aatcagcttg ccgtccagtc
     2641 ggacttccgc ttgtggagga gcaccgagca gcgcaaacgc cgtttcattg gctttggctt
     2701 cggtcggaac cgggacaggg ggcgcggcgc ttggggactt gcgctctgct ggctttgtgt
     2761 cggcgacggc tggggccggt tcgtcgccgt ccccatcgcc ggatgccgcg gcggcctggg
     2821 caaacagaag caggcagagc accagacctc ggcaaaccag cgcggcgaca tgcctcggat
     2881 actgcgtcga caactcctgg gtgacgcagt ttctagagga tgacattgcc cattattttg
     2941 tcacgccagg cgactttacg acaaggcgat cgcgcccgaa ttgcgacttt gtggatggcg
     3001 ctccgacttc accgccggca ccgccacgat ggggccgaac cgcaagcgcc caaagcgagt
     3061 gggacagacc gcggcacgat cagcacttat taagaggtgt cctaccgcgt agcttggcgg
     3121 ctacgtcgcc gcagatacca cttggtaatt tggtatttgg gctgcgcatt gtggagccgc
     3181 ggttcccatt ttttttcgca aaatacgaaa gaaccgacag caatcttgct tccttgccac
     3241 aagttggcgc ttccatctgt ccgcgtgcgg aagttataat gtgaagcggg gagcgagttt
     3301 ctttgggtcg tctgaggctc ttggtacgtc gtgtgagcgt gacggcgatg gctcggcaga
     3361 gtgtgctgct gggctgtctt gcgctgtcgg cctgtggcgg gcacccgatc aaggtccaga
     3421 tgacgatcgc caccgacgcg aacaataact ctccagtgtt gatttcggtg gtgtttccaa
     3481 aaaaccaagt gctgttcaaa aagctgctcg acatgaacgc caagcagtgg ttttcccaaa
     3541 gggagcaact tctgcgcgat taccgtcgcg aactcgatga aacgtacttc gagtttattc
     3601 ccggtcaaca ggtgccggag ctgacgcgca aagtcccgag caatgtgccg caggggatca
     3661 tgtttgtgaa ctaccagacc cctggatcac atcgctacac atttgacacc aatcagccgc
     3721 ttaaaatcgg attttcgcag cagagcgtga acctgcagtg atggggagtg ggaaccggaa
     3781 tgccgagcgc gcatgatctt ccaccaacga ttcagtggca cgagggaatg ctcctcgctc
     3841 cgcagcactt ccagcagctg gtgcagcgga cggaaaagct acttacgtac catgtaagct
     3901 tgggacttcc ctatcagttt ggcgtgatcc gtctgcgtta tgaatcgacg cagcttttca
     3961 cgggaacgct gcgcgtgacg gaactggaag ccgtgcttcc ggacggtctg gtggtgtcgt
     4021 ttaagaccgg accgtccgcc gagctgcaag tggatctgaa gtcacacaca gagaatgcca
     4081 agcacacgcc cattccggtg tacttggtgg ttccgatcga gcgcagcgga ggcggaaaag
     4141 gggacgcaga ccttggacgg tatctgtctg ttcccggtga tgaagtcgat gatgaagtga
     4201 gcggaggtcg accgctgcgg attcctcggc tcaagccgcg gctgcgtctg ctcgccggat
     4261 cggaaccacc cccaccgcgc tttgttgcgc tgcccctttt ggcagtggta ttcaaaaatg
     4321 gcgcgttcgt tcctgccgac tacattccgc cgcttctgcg ggtggaagtc gattcctctt
     4381 tgggggaaat gtgcgcgcag gtcagcagtc tgattcgcgc caaagcgaca tatctgttgg
     4441 aagtgattcg ctctccggcg attaccacca agccgactgc gatcgaagaa aatcggcgac
     4501 tcctttccca actgctcgtg aatctgccac cgtttgaagc gctgctcgcg acgggaacga
     4561 gtcaccccta tgcgctgtat gtggcgctga ccgcagtggc cggaagtgtc gccggagtcg
     4621 gcagtcagct gatgcctccg gtctttccgg cctacgatca cttgaacccg cggcgatcgt
     4681 ttggcgaagt cgttcagttt gtgaatcaat cgctcagcga cggaatttcc gaagccttta
     4741 ccgcgcttcc gtttcgcaat gaaaacggtc tgttttcgct tcagttcgag cgcgaatgga
     4801 ctcgtcggac tgtggttgtt ggtttcaaag gcgcgcccgg tgcgaccgat caacagatca
     4861 acgaatgggt cgcgagcagc ttgattggta cggcgagccg cttccgtgtt ctgcgcgaaa
     4921 gacgactcct tggcgcaagc cgcacggcga tcgatcgcgt cgaaggactg gtcgcgacgc
     4981 gcggaatgct gctgtatcag ctgcaagtga atccggattt catcgttcca agtgaaccgc
     5041 tggtgatcgg caattccgtc gaaaaaccgc caggcatgcg acctgcggag gcctatctct
     5101 atgttgcgaa caaagagtag ctttcgcttc ccatacgggg tgggtattcc caccgaggta
     5161 ggtaggtaag acaatggcta ccgagacctc ggaacagcta ttccttgtcg actactttcg
     5221 cgaattttac gcggcggttt tgcagcatcg catgcagatc gaaaaagcat ctgcacccgc
     5281 tgtggaagaa gacagtctag agattcgcga agccgacatt ttggacgagg aacgagttcc
     5341 gggggaggag ccttcgcaga tcgctcccga catggtgcaa gcacgcttgg tagagctgct
     5401 tgatacgcag cagcaagccg tgcaaagacg cggcgatccg cgcgagcaga agcgtttttc
     5461 cgaaattcag tatgtgatgg ccgcgatggc ggacgaagta ttcctaagca ttgagtggat
     5521 cggaaaggaa tactggagca cgcatcttct ggaagaacgc ttcttcaata cgcacgacgc
     5581 cggcacgcgc tttttcgaca acctggactc tctgcttcag aatcgcgatg cgacgcgcgc
     5641 cgatgtcctg gcggtgtact tgctcgcggt gtcgcttggt ttccgcggca agcatcgcag
     5701 cgtttcatcg gctccgcaga tcgcacacta tcgcaaccaa gcgtatgtga cgctgtttca
     5761 gcgcagtcca gcgctgcccg aaagcacgcc gctgtttccg caagcctacg cgcacacact
     5821 ggatggacag ccgccgagct ggctgccgca gctacggccc tggctgctgt ctttggttgg
     5881 cgtggcggtg atgtatctgc tggttgcgca tgtgatttgg gatcgtcgat cgacatcggt
     5941 gagcaatcgt ctggaagaga ttctgcagcg caaagatgac agtgccgcgt ccggcagtag
     6001 gaaagtcgac agccgttccg agctgccggc acaaccggac gcaccgaaaa cggaactgcg
     6061 aatacctggt tccgatgtga agacggcagc ggtgacaaac gaaggtacac ggggaagatc
     6121 gggaaccagt gccccgtgaa taggttgtga acagtcgata cgtggtgtca tttcgtgaag
     6181 tgatatgatg ggaatctgtt gactgcggtc ttgtgtttgg ctgcggtctt gtgggggaat
     6241 cgtggcaata cttgtacgct cctggctgat actgtttgta tcgctgctgc tgctcggctc
     6301 acagcctgct cgcggtcagg aggagagcgc atccgtctat gaagaagagg gagcgcgctt
     6361 gtttttgagc aagagctacg aaggtgcgat caagcagttt acccaggcgt acaggctcga
     6421 tccgaaaccg cagtatctgt ttaacatcgg acagtcctat cgcaagctgg gtcttgccgc
     6481 ggaagcagcg gagtactacc agcgctatct gcacgacgag caaaaaatcg atccgacgat
     6541 ctacgccgat ctgacccagt acatcgatca gtactacgct tcgcaggaaa aagccggtgg
     6601 gcgtccgaag tcgaccgcgg aacctccgga accggcgccg ctcttggccg ctgaagaaat
     6661 cgccgacgct gaagaactga tcgcgcagta cgtgcgcgac taccgcgcag gcaaccaagc
     6721 ggtcgccaat gagatcatgg atcagctaaa gggagtgctt cagctccgcc gtgatccgat
     6781 cctgatgtac cacatcgcca atggttacga tcaggtgagg cggcggcgtg atgcgctgga
     6841 atactatcgt cgcttcgttg cgattgagac cgctgatccg cgtctgcgtc gacggtcttt
     6901 tgaacggatt gcgattttga ccccagcccc gcctggtcaa aaatatctgt ggccaagcct
     6961 tgcgctggcg attcctggtc tggccggcgt gctgaccggg gtcggtctgt atgttcaatc
     7021 ggatagcaac tttcagcttt ttcagaacga gtcggacgaa cagaaaaagc gggcgctgcg
     7081 tgacaaagga acgggtcttt cgatcggatc gacggctgcc tacgccgctg gtggcgcgct
     7141 gcttgcgggt gcaggtgtgt ttgcgattgt tgcactgaaa aaaggaatcc ggcagagcag
     7201 cgaagacaag ggaacggaaa aaaaagaaac gcagaagcgc agcaccctga ccggagcttt
     7261 gaacccgctt ccgggcggaa tcggactttc tttgggaggg aacttttaat gcttaaaact
     7321 acgctatctg cactgctgtt tgcttcgctg agtcttcttt ctggttgcac gctgacgatg
     7381 agctacaacc agtgcgtaga gaacgcggac tgcaaaaatg ccgacgggac gacgggattc
     7441 tgttcatcgg atcagctctg tcttccaagc acgccgctag aacggctgtg cacaagtacg
     7501 attcctgaaa ttccttcgcc caatgcggtg cacatcggcg cgctgctgga tctgcgtgat
     7561 aatggcgggc gaccgatgga agcgcaccgc tacatcgcgc tgaagctggg tgtaaaacag
     7621 atcaacgagc tggtgcagct tgacggctcg cgaccgctcg cgctcgatgt ctgcaacgtc
     7681 ggggtgtcgc cgattgatgc ggacaacgca acgaagattc tgatcgagca gcgcaacatc
     7741 gttgccatca tcggtccaaa cagcgcggcg gcagccacca acatgatcgg gcggatcaaa
     7801 gccgctggtg ttcccaccat ttcgccgtcc atcatggaca gcggaatcgc cgatgctgca
     7861 agccaaggtc tgttctaccg cctcactccg gttgagcggg aacaggggct tcagcttgtt
     7921 cgggaactta ccacgctgtc tggtggcaag agcacgtcac agctcggtct tttgtcggta
     7981 tccgattcct acggtgaaaa cattcgtcgc aagttcacga cggaatggca gcgcaaagat
     8041 ccggtgaaca acaagaccga ccgtttcttt tcgtacaaag aaaacgatgc caacctgctg
     8101 gcggtcacga ccaagacact gcttgatgcc aagccgagct ttgtcgttct gattccgggt
     8161 gcaaacagtc agcaggtgat tggtgggttt gcggatctgc cgtttgatcc gaaaaatccc
     8221 gcttcctttg aaagcaccac ccaagtgctc ttgtcgtcga gcggacggac cggcgagctg
     8281 attgcgatgg cgcgcagcgg tgatgaccgg atgaagaatc acttgtcgcg gatcaccggg
     8341 gttgcaccgc tcagcttcat cgacagccag gaaggcgcgg actttaagtc cagcttcgtt
     8401 gcgaccaatc cgggcctgcc gctcgaacag gattacatgg ttgggtacac ctatgatgcg
     8461 ctgttcgtgc tgggggcggc ctctgcttcg tttaaggggg cgagccgtcc ggatcagatt
     8521 ctgtcggtac tgcgaagact caatggcact gcgaactcgg tttcgctgac tccgcgtctg
     8581 tttaacgaaa cagtgcagaa gctcgcgcag caaccaaatg attcgttcac gctgctggga
     8641 gtgacgggtg cgattcgctt tgcgaccgac ggtggacgtg atccggttct gctcgaacgg
     8701 tggaagatcg atgcgacgaa cggggtgtat gtgaacaccc ctgcgatgta gcgctttgcc
     8761 gaagggaaac gcttcgtgct gatccgcatt ttcatagcag ttctgatcct ggctgtgctg
     8821 gcagccggtg tatttctgtt tttgcgctat cggcggaaac agcaagaggc gtcccctgaa
     8881 gcggctccgc ctccacccaa agatccagaa cgcgccaagc ggattgctca gctaaaagcg
     8941 agcctgacca cgcttgtcaa tgaagagcta cgccagctag accaaaatgt cggcggtgcg
     9001 aacggacgct acggaatgcc gtggtgtctg gtcgttggtg aaaaaggatc gggacagagc
     9061 accgtgctgc agaacacggg cctacacata ccgttccgtg gcgcaagcga tgcactggcg
     9121 caagcgggaa actgtcggtt ttggttttat gacaaagggg tcgccatcga tctgggaaca
     9181 gagatatggc aggacgagga gctgtttgcc cacttctgtg gtcttctgaa gaccgcgcgc
     9241 cccaagcgtc cgctcgattc cgttttggtg gtgctgcctg cgacgtcgct tgtgggggaa
     9301 gacaaacgga atccggaaca gctaaaggaa agcagtgagg agctgtatca gcgtctgctc
     9361 aagcttcagc agaacctgac tttgcgactt ccgacttacg tgattgtcag caaaggggac
     9421 accattccgg gctttactgc gttctgcggg ggtctttccg gaaaccagcg cgagcagctt
     9481 gcggggtggt catcgccgta tccgatcaat gttccttacg atccgtcgtg ggtcgatcaa
     9541 gcctttgccg aactttacag aacccagtgc ggactccaga tcgatctgct ggcgagtgct
     9601 cggctatcgg acaaggaaca ggacgcagcc tttcttttgc cacgcaacac ccaggcgctg
     9661 cgcgagccgg tcaagctggt cttggatcag ctcttccggg tcaatgtcta cagtgaatcg
     9721 aatcttctgc gcggtctgta tctgtgtggt gacgcaacca ttgccagtgc gatggtgcgg
     9781 cagaatccgg aagaggaagg ccagaaaaaa cccgtctttg tccatgatct gtttggcaaa
     9841 aaactgttcc cagagagtgg tctggcgcgt cccctaaagc gtggtctttt ggatcagggg
     9901 cgcgcactgc gtctggccaa agcgggtctg gctgcgtcga ttgtggtgag cattctgctt
     9961 ctgtggagcg cttacgcgaa cctgtccaaa gatgtccgcg cggtgatggg atttttggac
    10021 aaagttccgg tggaaaccac gctgtcggtt gcacaggaca gagaccgctt tgcgctgcag
    10081 acccaggaac ttttgtcggc gataggcagt gtggcgacaa gtcgcttgct cagtgttcgg
    10141 ctgccttcat cttggcttag ctcgcttgat gatgaagtgc aggaagccat gcgccgatcg
    10201 ttcgagcgcg tggtgcttgg cggtctgcat gaaggtctgt tgctcaaagc caagcaggtg
    10261 ctgggactgg aaggagactt gaccgcgcaa gcgcccagcg acgagccacc gccagcaggg
    10321 gagcccggat cggggcgcac cggatcggcg agctctgagc gcctgacatc gaaaacggtg
    10381 caggtgctgc cgcttcagag catgccggag tatcaggagc tgcgcgagct ttccaagtcg
    10441 tatctggagt ttgtccaaaa cgtcgatacc tacaaccgct tgggaagtga acaccacaaa
    10501 cgctttgata ccgtggtgcc tctcgtcaag tatgtgtttg gcgttgagct gcagccggtc
    10561 tttctgcgaa atgcagagct ttatcaggaa gcacttgcca acgcgaccag tcgttcgttt
    10621 gacatggctt tggtcaatcc gaaagtccag gcgcgggcga agacgatctg tcgggcgttg
    10681 catcggcgtc tgttcattga caatgcggtg gcgattgaaa tcgacgaagt gctcgcgcat
    10741 gtcagccggc tcaaagcaga gagcgaaggc ggtggtgcag accttccgat cttgaccgag
    10801 ctgcgcgatg tgctggtgcg gttggatcgt gatctgtcgc gcaccgaact gcagtggatg
    10861 tcgaaagact cgctcgatct gggcgagccg atgagcgagc ttctggccgc gctgcgccac
    10921 ttttcccccg acggaaaaat gagcgatgag ctgcggcagg aatggcaggc gtcgcatcag
    10981 cagcttcgcc gccgactgct ggaagttcag cttccacagc ttggactcat catccagcgc
    11041 gacaacgaaa aacaccgctt ggtgatgacc caaggtgtgg tggatctgaa agcgaacctg
    11101 gaaacatttt tggcgctttc gtttgtgcag cagaatctgg aaaagccgca cgcgcagaac
    11161 tcagaaggat cgtaccgcgt ggtctggaat gatgatctgc tgcgccaggc cacgggcttt
    11221 tcggaagcct atgacaccta tgttcgcgac aggcttggac aggtctatcg gcacattcgg
    11281 gaagtcgtaa agagcacggc gctggaacgg ctcggacaga atatgccagc actggtggcg
    11341 caggcgcggc gtgaagagcg ggtactgcgt ttttccggtg atgtcaacgc gctgcaagac
    11401 gagctaggaa acgaagtcgg ggatctgaag cgggtgtcta cggtgctgcg gcagattctg
    11461 gaaacctacg atcgcttgtc gctcaaggcc gcgcacagcg agctttatca gcagcttcag
    11521 gaagactcac gcgagattct gcgccggatc gatctgctgc ccgaacgcga agagatgtac
    11581 cgggtgtcca gcaagcttgc gtcttggcga ggcgagcgac ccccggtgct tgcggcattt
    11641 gatgtcgaag acgccgatgc actttcgcag tacataaaga cccagcgcaa ccgcatcaag
    11701 ggttgggggc gcgattatgc caaggttccg gtgtctctgc tcgatggcct atctagtgcg
    11761 cgcagcgacg atccactggt tgggaaatgg cgcaacatcg taaaccagct cgagtacttc
    11821 gagcggatga ctccgggcaa ctcggtcaag gaaatggagt cgttctttga caccacgctg
    11881 cttacggtga caccggaaaa ctgtctggac aagctgccca ggcagagtgg tgacaacacc
    11941 gactttttcc tgcaatccaa agcgcgcgtc catgaatcgg tacgcaagcg ctgcttggtg
    12001 tttgcggcgg aaaagattct cgaactgtat gaacacctgg cgcgtcgctt caatcaagac
    12061 ctcgctggga agtttccgtt tacccgcaat ccgcagtcgc tggacgatcc cgatgcactg
    12121 ccgcgcgatg tccgtggctt ctttcaggaa tacgatgagt tcatccggca atatgacgcc
    12181 tacgtaggtc gtcgcgaaac gcttggtggt tccggagaag tcggtcgaga ggtgaacgcc
    12241 tttttgcagt cactgcgcga ggtgaggcct ttctttgcgc cgctgcttgg tgacaggggt
    12301 gagcaagcgc gttaccaagt gtcggtcgag taccgcatca acaagagcaa tgagataaac
    12361 ggtcaacaga tcgcagagtg gagctttgcg accggtgatc agaaagtcga agacggccag
    12421 ggattttggc agatcggcga tcgggtaaaa ctgatgatgc gctgggccaa agatggtcca
    12481 tatgtgcctg caccgacggg acaaccgcgc ggcgcgagcg ttgattcaaa cgatgcgatc
    12541 gcgtttgatt ttgccggcat ctggagtctg gttcggatca tccgcatgtt ccgtgcgtcc
    12601 cagactgatc tgcgcaagtc gctcgatcgg caaccgcatg tgctcaagtt catggtcgat
    12661 atcgccgaaa gaaaacggag cggtcgcgtg cgattgcttc cggattccta ctacaccaac
    12721 aagggaagta cgccgactgg cgcggcgaag atggcgtttc tttacaacaa agcggtcgtc
    12781 tttgttcgca tggttctcaa tccagcagag agcaaagagc tgctggtgat tccggttgat
    12841 tggccgacgc tggcgccgct gacgcgggcg cgcagtggac agtaaggaaa gggtatgtct
    12901 gaaaagcctc ccatcgacat cgagtctctg cttgccccga tctcggaacg agagccggca
    12961 ggtgagtggc tgcgctatga agggacatac gatgcgatct ctgaagcgcg tcgcgaagac
    13021 gatgaatcgc tgccgcaagg aatctggcag accaagctta agcgcgccga ctttgccaag
    13081 gtgtcgcagc ttggacagaa agcgctgcgc gaacggagca aggatctgca aattgcagtc
    13141 tggctctgcg aggcgtggct caagctgcat ggaattccgg ggctttgtct gggaatcgat
    13201 ctctgcgccg ggcttttgtc tcgattctgg ccgacgctgt atccgctcat cgaagacggc
    13261 gacgcgagct accgactggc accgattgag tttttgtcgc aaaaagtggc gctgcggctc
    13321 aaagagattc cgctaacaga gagcgtgtcc gagcgcccgc cgctgacttg gaatgactgg
    13381 gaacgggcac tgcgtaacga aaaagcgggt gccggatccg atgagatttt gccggcgcag
    13441 tttctggcgt ccgcgtccgc gacaccgagc gcattgtttg ccgagcgatt tgctgagctt
    13501 gaagcgctgc aagactgcgc aaagcgtctg gaagcggtgg tgctggggtt tgaggaagag
    13561 caaccatcgg ttctgctttc cctgcgcaac cttgtcaccg acatgcgcgc gctgattggt
    13621 cagtacgcac cagcagcacc tatcgcagcg gcgcattccc cagacactgt accggtttcc
    13681 ggttcgcaag acggcaccag tcccaagaca gcggaaaaca gagcacacgg cttggaggca
    13741 tccgtgcagc ctggcagtga aatgagggag gaatcgagta cgggcccgat tcgcagtcga
    13801 gcccaagcat atcaacgact tgtcgaagcg gcggattacc ttcagcggac cgagcctcac
    13861 agcccggtgc cgtaccttgt aaaacgggcg gtcgcatggg gtaacatgga cctgattcag
    13921 ctactcgcag aacttgtggc aggggatgct gatcgtcatg tgatctatgc gctgctgggg
    13981 gtgaaggctc cgcactagtt tggaaacacc aatgtcattc gtgagatgcc aaggattggc
    14041 cctggcgtct gtggttacaa ccggggaaca cccccaattt ggctgacgtg agggaacgat
    14101 gtctgagagt acccagcaca aactcgaccg tgtaaggccg ccaagggtcc agatcactta
    14161 cgatgtggag atcggcggcg ccatccagat gaaggagctt ccctttgtcg tgggaatcct
    14221 tgctgatctt gctggtcagc aggatgcgct tccgaaactg aaagatcgca agttcacgca
    14281 gatcgatcgc gacaacttta accaggtgct tgccaacgtg gcaccaaagc taaagtacag
    14341 cgttcccaac cagattcaga aggaagggaa gccagtaggt gtcgatctga cgttccatca
    14401 cctggacgat tttcacccga cctcgatcgt gaagaacgtt ccgcagctac gcgagcttct
    14461 ggaaatgcgc cagcggcttg tcgacctgct gaccaagctg gatggaaatg acgacttgca
    14521 gacccagctt gcgggcctgc tcgccgacaa agacaagttt ggtgcggtca aggcgctcgc
    14581 cgccaagagc gcggtttcga gctaaggagg ggttgccgtg gctgagaatg aaacgactac
    14641 caagacgacg acgaacatcc tcgatgacct gatccgcgat ggcaagatgg ctcgtgacga
    14701 gaaccagctt gcgcactcgc ggacactgat cggcgagttc gtgaacacgg ttgatcccac
    14761 tgcggttgca cccaccgttg atctggcgaa agtgattctg gaaaagatcg cgcagatcga
    14821 caagatgctg accgcgcagg tcaactccat catgcacgat gagcgcttcc agaaactgga
    14881 aggttcatgg cgtggtctga actacttggt gatgaacact gagacttcga cctcgctgaa
    14941 actgcgcgtg atgcatgcga cgaaaaaaga gctgctgagc gatctggaaa aagcgtcgga
    15001 gtttgatcag agcaatctgt tcaagaaagt gtatgaggaa gagtttggta ctttcggtgg
    15061 cgcgccgtat ggttgcctgg tcggtgacta tgaattcggt cgtcatccgc aggacatctc
    15121 tctgctcggc aagatatcga acgtcgcagc ggctgcccat gcgccgttta tcagcgcagc
    15181 cagtcccaag ctgttcgata tggagagctt caccgagctt ggcaatccgc gcgatctggc
    15241 gaagctgttc gagagctcgg agcttatcac ctggcgttcg ttccgtgagt cggaagactc
    15301 gcgctatgtt gcgctgacgc tgccgcgcgt gctgggtcgt ctgccttacg gcaacaagac
    15361 ggttccggtg gaagagttcc agttcgagga agacaccgat ggcaaggacc acagcaagta
    15421 cctgtggagc aatgccgcgt atgtgatggg ccagcgcatc accaatgcgt tcgctctgca
    15481 caaatggacg gcagcgatcc gcggtgtgga aggtggcggc attgttcagg gacttccggc
    15541 acataccttc aagaccgacg aaggcgacat tgcactgaag tgcccgacgg aaatcgccat
    15601 caccgatcgc cgtgaaaagg aactgtcgga tctgggcttt gtctcgctgt gccactgcaa
    15661 ggacaccgac tatgcggcgt tctttggtgg acagacgacc aacaagccga aggtctacaa
    15721 cacgccgcag gccaatgcca atgcgtcgct gtcttcgcag cttccgtaca tcctggcgtc
    15781 gagccggttt gcgcactatc tcaaagcgat catgcgcgac aagatcggca gcttcctgac
    15841 gcgcgaaaac gtccagaact atctgaacaa ctggatgtcg agctacatcc ttctgcgcga
    15901 tgatgccggt caggaaacca aggcgaaata tccgcttcgc gaagcccgcg ttgacgtgtt
    15961 tgacgttccg ggcaagccgg gtgtgtatcg cgccgtggca ttcctaaagc cgcacttcca
    16021 gctcaatgag ctgaccgctt cgattcgctt ggtggcagag cttcctccgc ctgccgctgg
    16081 ttagtcacac aagtgctatg aaacgcctct gccttgtggg gacagaggct taaggggaat
    16141 tgagaaatgc agaacatttt ctgggaaatt gaaggcatca agggtgaatc gacctctgct
    16201 cagggtaagg acatgatcga gctcctttcc ttcaaccacg gggttgcgat gccgctcacg
    16261 cacggtccat caaacaccag ccgtgcgtcc ggtcgctgcg ttcaccagga cttcactgtg
    16321 accaagcacg ttgacttggc ctcgcccacg ctgaatctca agtgctgcgg tggcgaagac
    16381 atcaagtcga tgaaagtgca catctggaag gccgactctg ccggcaagcc gatcgagtac
    16441 atcacctaca cgttcgacag ctgcattctg accagcgtgt cggtgggtgg cggcggcgat
    16501 cagccgatcg agacgctgac cttcagctac aagaagatca cctgggatta cgcgcagcag
    16561 cttcagaagc agccgggtgg caacaagggc aagagctcgt ccaactggtc gctggaagag
    16621 aacaagggca gctaatcgcc tgcttctctg tgtcaagagg tgtggtccgt agatgccgct
    16681 tccatctaaa aagcacgtcg cagcggttcc gctgtttgag cggctctctg atcgaaaacc
    16741 agagcagccg cgcgagccgg ttccgctgcg cacgctttcc tatccagagc tacttgactc
    16801 ggtgcgcgga gaagtcacac gactcctcaa cacccgctct ggactggctt cgcatgagct
    16861 gctcgagcgg gagcgctctg tcattgattt tggggcgacc gatcttctct gggtcaatcc
    16921 gtttgatccg gaatcgatgc ggcacctgac acgactcctc gtgcagacca tcgaaaagtt
    16981 cgagccccgc cttgcgcatc cgcgcgtgac catcgtgcag tttgttcaaa cagaatcgcg
    17041 actcattttg caggtcagcg gaaccctggt gatggaacaa ctgcgtgagc cggtgtcatt
    17101 ccgggtgacg gcaagtcctc gtcttttgga gcacagaagc gatgagcagt gacgccaaag
    17161 acgagcttct tcgcgcgtat cagcgtgagc ttgcgtatct gcgcggcatg ggcgcagagt
    17221 ttgctcgcaa gtatcccaag attgcggcgc gtcttgatct gtcggcagag acttgtgccg
    17281 atccgcatgt cgagcgattg atcgaaagct ttgcattttt gaccgcgcgg cttcagtcgc
    17341 gcctcgatgc agactttccg ctcgtgacaa cgtcgattct ggaatgtctg tatccgcatc
    17401 ttttgcagac cattccgtcg atgtcgattg ctcattttga tgtcgatccg gaacagggac
    17461 tgccgcttgg tggtttcaca atcggaaagg gagcaaagct gtttgcgcag accgataccg
    17521 gcgcgctctg tcgcttccgg acaggtgccg atgtcacgct gtggccgatc tcgattgttt
    17581 cggcgcagct tgaatccacc gatcgctatc cctacctgga tgcatatcca aagggaatcg
    17641 cgatgctgcg gctcaaactg gaatgtcaga ccgacaactt tggtgagctg gatctgcgta
    17701 gcatccgcgt gtttgtgaat tcctccgcag aagtggcatc agcaatttat gatctgctgg
    17761 tcgcatccac cgtcggtgtt gttcttcttc ctgatggtcg tccggaagtt cagtttttgg
    17821 ggccgagtgc gctgcgtccg gtcggtcttg aaaaggaaga ctcccttttg ccgtctccgg
    17881 agtatgccca tccggcctac agactgcttc aggaatactt tgtctttccg cagaagtttc
    17941 tgtttttcga catcgacctg cccaggattc agcttgcgcg caagacggcg gacgttctga
    18001 ttctgttatc ggaagcgccg ccgcgttccg tcgcagtgac caagagtacg tttatgctcg
    18061 gctgcgcgcc gatcatcaat ctgtttccca aaaccgccga gccgatccgt ctgacccatc
    18121 ggctgccgga atatccgctg gtcgcggaca agcgcgaaga aaagacctgc gagattcact
    18181 cgatcgacaa ggtatcgcag agcgcggacc cgaactcgag ccagaacatc gcaccgtttt
    18241 tttcgttcaa ccatcccgca agcggcgagc agcatcaggc gttctattac gcgcagcgca
    18301 gaccaaccgg acgcgccgat ttggggggtc tggataccta tctgtcgttt gtcgacatgc
    18361 gctttaagcc gacacagcca gcgacgcaga ctgtgtttgc gcatacgctg tgtaccaatc
    18421 gcgacttggc ggcccagatg cccgcgcggg cgcggctcca gatggaagat gccgcaccgg
    18481 tcaaggccgt gacctgtctg tccaagccca ccgaaccgat cactccgccc ctgggtggag
    18541 ctgcgctgtg gcagcttgtc tcgcatcttt cgctcaatca cttgtcgctg atcgggggcc
    18601 gggaaagccg cgatgcgctg cgcgaaattt tgcgcctgta tcgtatgagc ccgacgacag
    18661 cgacagaaaa tcagattttg ggaatcaccg aacttcagtg cattcccaaa acgctgcaag
    18721 ccggtcgcga tgcgtggcgt ggttttgtgc atggacagga ggtccagatc acgtttgatg
    18781 aagggctgta tgttggacac agtgcgctga tgatgtcggc ggtgctgagc cgattcttcg
    18841 ggctatatac cgctgtaaac tcgttcacga tgctggttgt ccgcagccgg cagaggcagg
    18901 aggcgtggaa gaaatggcca ccgctggcgg gcgcccgcaa ccttctgtaa tcgaggagct
    18961 cctttcggag ccacagcgct tcaatttcta tcagcttgtg cggctgctgg aaatgatgga
    19021 gccggattct gtctcggtcg ccgaaggcgg agatccgacg gcagaggcgg ttcgtttccg
    19081 ttccacgttt tcactcgcat ttccgccaag tgatgtggtc gcgatcgaaa atgtgaagac
    19141 agaatcgata gaagatgagt tcgagcagct gatccgcaga gccaacggag aagagaaaac
    19201 gccgacggta acggtcagta tgcttggcct gggtggtgcg cacggaccgc tgccgcattc
    19261 gatgaccgaa tgggtgctgg aacgaagcca agcgcgcgac ttttccctgc gcgattttct
    19321 ggacatcttc aatcatcgac tggtgtcgct gctgtttcgg gtgcggcgca gctgccgact
    19381 ggggacggag tggacatccc cggatgagca ccacttttcg cgctttctgt acggtcttat
    19441 gggaatgctt acgccgggtt tgcaagaccg gctcaagatt cctgatcgcg cccttttgcg
    19501 ttacagcgga cttttgacca agcagccgcg tgatgcagcg ggtctggaaa cgctgctcag
    19561 tgatcacttc caagttccgg tgatctgtca tcagctgcgt ggtgcgatgc gaccgatcga
    19621 agaagatcag tggaccaaga tcggtctgtc tggacaaaat cagatgctcg gtggtcaggc
    19681 gctgcttggc acgacgattt gggatcaaca ggctggcatc gagctggaag tgggtccact
    19741 gccctggaat cgctatctcg actttcttcc ggggaagtcc ggactgacgg cgctggtggc
    19801 gattgcacgc tactacgtgg gtccggaatt cgatctgttt gtctcgctgg tgttgaaatc
    19861 ggaagacatt ccgcgggcac ggctcgccta tcccagcaaa gagacagaga gcgcaacagc
    19921 acccgcaggg gtgactgctc cggcaaaggg tgcacttgct gcgaaagctg cgtcatcaca
    19981 aggtcagctt gcgacgacag ctccggtctt ggggttgact tcgtttctga cccacaacag
    20041 caagctgcgt ggcgttccca aggttgcgct tggcgtggtg caaaggtaca gcgatgaacc
    20101 gttgagggct tccccgtgag tgagcacgac tttcttagcg catcgactcc cgctgcggat
    20161 gccttggtgc tggaatcgtt cgatggacag gaagagctgt cgctaccgtt tctgtattcg
    20221 cttcagtttc tgtccaaaga tcgcaagctc gacttcaaga aaatgatggg aaaggcagtc
    20281 acgatcacgc tcgtactgcc ggacaagtcg aagcgctaca tctgcggtct ggtcacgcgc
    20341 tttgttgagt ctggctacga ccccaggcac acgatctaca tcgcagagct gcgtccgtgg
    20401 ctgtggcttt tgacgcttca tgcagacaat cggattttcc aaaacaagag cgttcccgag
    20461 atcatcgaag aggtcttttc cgatctcggc ttcaaagact tcaaaaagag tctgaaggca
    20521 acatacgcca aacgggaata ctgcgtgcag tatcaggagt cgagctttga ctttgtctgt
    20581 cggctgatgg aagacgaagg aatcttctat tacttcgagc acagcgacgg caaacatacg
    20641 atggtcttgg ccgacgacag cgatggatac tgcaagtgta cgggatcgac cgatgttggg
    20701 atgggacttg atgatacgcc caacaacctg cggaactgca cgtttgagga atcgctggtt
    20761 acgaccggat acgcgctgac agactacaac ttcgagattc cttcgaccga tctgtatgtc
    20821 aagaccggcg gtgggagtcc ggcgcttgtg gtctacgatc atcctggcaa ctacgccaaa
    20881 aagaacgacg gtgaaaagct ggcaaaactg cggatcgagt cggtggaaaa tggacagaag
    20941 gtcctgcggg ccagcagcaa ctgtcgtggt tttacctcag gtggaaagtt caagctgacc
    21001 aaacacgatc gcagtgattt gaaccaagag tatgtggttc gcagcgtgca tcacactgcc
    21061 gatcgcgcaa actatcgcaa ctccatcgaa gcgctgccgg cctctgtggt gttccgtcca
    21121 ctgcgccgaa cccgtcgaca gcttgccgta ggcagccaga ccgcaaccgt ggttgggaag
    21181 tccggagaag aaatttggac cgacaaatac gggcgcgtaa aggttcactt tccctgggat
    21241 cgcaaaggca aaaaagacga aaacagttcc tgctgggttc gggtgacaca gcactgggct
    21301 ggcaagggat acggaacgtt tttcctgccg cgcatcggcc aggaagtggt gattacgttc
    21361 attgatggga atccggacag gccgcttatc accggagccg tgtacaacgc cgaacagacg
    21421 gttccctatg cgcttccgga caaccagacc aagagcacga ccaagtcccg taccagcaag
    21481 ggcggtggtg gttttaacga aatccgactc gaggacaaaa aggattcgga agaaatctat
    21541 gttcacgccc aaaaggacat gaacatcgag gtcaagcacg accgcaccgc caagatcgac
    21601 aacgactgca agaccgaaat caaaaacgat cgcagcgtca aagtcgacaa caacgacagc
    21661 ctgaaagtca aaaagaaccg cgaagtcgaa gtgagcgaag gtgacgagag cctgactgtg
    21721 aagaaaggga atcgcacaat caccgtcgac aaaggcgatg aaaagcacac cgtcaagggc
    21781 aagcgcgacg tcacggttca caaagacgaa aagcacgaaa gcaaagccaa gttccagcac
    21841 gatgtcacca aagactacat cctgaatgtc aaagggaatc tgacgatcac cgtcgatggc
    21901 gacatcaaga ttgtgtccaa aaaaggaatc aaagcagaag ccactgcggc gatggatttc
    21961 aagtccgggg ccgatatgaa gttgaaagca ggcgcgaact tgaaacagga agccgcggtc
    22021 aacatggaca taaaggccgg cgtcggaatg aagatcgagg gcgtgaacgt cgacgtgaaa
    22081 gcggcggcga tggcgaagct ggaaggtgga gcgatggcgg ccataaaggg tgccctgctg
    22141 aagctcaact aacgcgatga accacaaagg acacgccgct gagcgcaaga cgtaacaaaa
    22201 tgagcgacgc agagaccagc gcaagtgcca acccgccggc cccaactgag cttgtggaac
    22261 acgatgaaga gggtcgtgtc accatgcagg gccctttgca tgaagggaaa aagcacggcg
    22321 aattttgctt ttttgccgca gggaagttga cgcaaaaagc gaacttcaaa aacgatcttc
    22381 tcgacgggga aacggtcgat tactttccga atgggaaggt ctcgcagcgg ctgcattttg
    22441 cggctgggaa aatgcacgga ctgctgcgcc gtttcgatga tcaggggcgt ccagagctta
    22501 tcgctgcgta tcaagcaggg gaattgcatg gcgatctgct gacctatgaa aacggccaga
    22561 tggtcagcca gcagcactat gtaaaaggga agaagcacgg cgtcggaatc atctatggtc
    22621 cagacggaca accgaccctt tcggccaatc atcagaacga caagcggcac ggtgaagcgg
    22681 tctactttgg tgcgcatggg gtggtgattc ggcgcgaacc gtatcaggaa ggactgattc
    22741 acggacttgt gcaggagttt cttcccgacg ggcgactgcg tgaaacgtgt cagtaccagg
    22801 ccggcaagcc gcatggtgaa cagcttgtct actatcccag tggcaagctg cagaagcggg
    22861 cccgttatga acagggtgcg cccagcggaa atccggaact attcgatgaa aaggggcgcc
    22921 ccctaaagcc tgccgaaaca ccggacagcc cgtcgatgga agccaaagcc gcggaagccg
    22981 cgaagcagtc tgcactgcgc ggactgatgg atgtggcgcg caagcggatt gtggaatctg
    23041 cggcaccggc tgtgatcgcg gccatcaaag cgccggaaat tccggaagtt cccgagctgc
    23101 ccgctgcgcc ggaaataccg tctgcaccag agcttccgca ggcgccggaa ataccccaag
    23161 cacccgaggt tccggaagag ctgaaactga agccaccagt ggctgccgaa aaagccgcgc
    23221 ttgcgcagaa ggaacttccc aagccgggca agaaagcgcc gccgcctcct ccgccacgca
    23281 aaggactgat tctggaaccg ccgaacatcg gtacgttgcc ggctgaaaaa accaatttgc
    23341 cgcgaggaaa gtgatgccaa acgcagtctg tatgggagcc atgctcaagt gttccttcgg
    23401 tgttgcgcct agctcgctcg tggtcttgcc gaccaatctg gtcatggggc cgcagccggt
    23461 ggcaaacatc atggatggca agccgatggt gaacatcctg ccgttcggaa tgtgtcagtc
    23521 gatggccaat ccgatggtgg cagctgccac agcagcagcg atgggagtgc tgacgccaat
    23581 gccgtgcatt cccaatacgc ccgccccttg ggtggttggg gctccgacgg tgatgctggg
    23641 caatatgccg attttgaaca acagcgcgaa gctaatgtgt ctgtgggcgg gcgtcattga
    23701 aattctgccg ccgggcattc ccactgtgat cacgccatag gagtctcgat ggccaccagg
    23761 gtacgcaagc cggatgctgc caagaccgct gagcctgccg cggaaccagc cgcaacgacg
    23821 cgccgtcggg cgacgcagag tcgaacggct gcaacgcgcc gcaccgccag ttcatcgcgg
    23881 agccagcgga ttgtgcttcc ttccgatctt gcggaagcgg aaaaggaagc cacgacaccg
    23941 agtgtggccg attctgcacc tgcattggaa gtggcagaga gtcatcaaat cgccccggtt
    24001 gcaaaagaag aaatcgcagt aagtccaaca ccgatcgcga gcccggatgc ggtcgcggac
    24061 gcgccttttt ccaaaaaaac atcggcgaaa tcggtatctg aaaagaaaac caaggaatcg
    24121 catagcgaag tccgttcaga gtccaaagat gccggagttt cgacggaaac gtcgtccgtt
    24181 ctgcagcgcc tgtccgaact tcagaaactg gtaacggatg gtcaagatcg cagtctggcc
    24241 gaactacaga agacccagac ccagatggtt cgcaacctgt cgggtctgtc gcgcgatgtc
    24301 gacaagctgc taaagcatgt gcaggaactc caatcgcggc ttgcgcaaag tgagcttgtg
    24361 ctttcgcggg ttgaatctac gctgggtccg ctgtcgcgcg gggtgtcgtc tttggtcatg
    24421 ccggccaagc cactggaacg tccgctcagc cccgatgtat tcgaaaaatc gctgggtctt
    24481 gcgctggccc ctattttggc agaactggtg gagctgcggg ctcagcgcgc gcgcgacagc
    24541 agcctggcgc aggtcgaaca gcagattttg atgacggaac ttcggcgact gcgtaatgtt
    24601 ggactgagtc agattgaacg gaataccgat ccgctgtctt cggtgggaaa aacgggtcgg
    24661 cggacgcctt caaagttgtg atttgcagtg ctttggattg gcttaggaat cggaatcgta
    24721 ttgcgtcctt aacgacttgc ttatttggga gggaaagatg agcggcaaag gcacacagag
    24781 gcgctggact tgccatcttt tggctctgct cgcactgttg gtcagttcgt gtagcgtgcc
    24841 tgatggtttg atggtgacgc tgcgtgcccc cgacggggtc cgtctgcaaa gttacgtcat
    24901 caaagtccag gacagagaga gccgcaaggt cgtctttctg tcgggaattc agacgatcag
    24961 tgacaaacga acgctggcca ccgatccgct caaggtggcg ctgccgtttt cgcagcacgg
    25021 caagttcttg ctgcaagtgc tggccgccaa cgtcgccaac gttgaatcgc tgccgcaaaa
    25081 aggcaactcc gaaccgcagt acttttttgc ccgcatcatc gacgtgggtg cctatcagga
    25141 cgtcgaagcg aagcttctgc cggtgccgcc ggagttcgat gtcgacggag atcactttcc
    25201 cgacgcggtg acgtggaccg cacagaatcc ggaagctgcc gcgctctatg ccgatcagcc
    25261 gctggtgctc gactgtgtgg acgacgatcc tccgcccggt gatctcgatc cggtgcggct
    25321 gcgcagcttt gatattcatc ctttggcggg gcgttcctgt aacgtcaggc tgcgtccttc
    25381 gcacgcgcca aatgcgatgc cgctgccacc gcttcagctc tttgatgcga cgtgttcggg
    25441 cgagccgcgg gcctgcgaag acaaagacgg cgacggcgat ccagaaggca gcgactgcga
    25501 cgacaacgac aaaaatcgtt tccacggcaa cccgcgcccg cgcaactgct gcgagtgcac
    25561 cgacgtcacg agttgcaaaa ccaaccacaa caagcgcgcc gacatgacgc tgtgtcagcc
    25621 caagcgctgc gacacgacgt tcgactatga ctgcacgggt ctttctgtcg actgctttac
    25681 cgacgatgac tgcgatggct attcgcccaa caaccccgat gtgaagctgc gtgactgcga
    25741 tgacaccgac gcgcgcgtcc atcccaatgc gcagaagctg tgcgatccgc ccgacggtgt
    25801 gatcaaagac tgggcctgcg acggacgacc gagcgctggc tgcgtcccgt gcgacctcga
    25861 tggcgacggc tttcagcgct ccgaagtgtt ggcgggacag acgtgcccga cgataaacta
    25921 caagaactcg gggcgtccgc tcgactgtga tgacaacgat cgcggcgtct tccccggcgc
    25981 gctgacgtat caggggtcgg cgatgaagta cggaacgctg gatgcgtcat cgcgcgcctt
    26041 caatgtgctg tcggcgatgc gtggtctgtg tcgggcgacc gatccggcgg gaagtccaca
    26101 gaacacaagc tgcgacggcg tggccagcgc gcgacagggc tgccccaccg ccgcttgtga
    26161 tgccgacggg gacggttttc ccaaaccgct cgcgagctgc atggtggccg gacagccgac
    26221 ggattgcaac gacaccgatc cgacgatttt ccccggtgct ccgctggcct gcgacggcaa
    26281 agatcacaac tgcgacatga tggtggatgt ttgcagtttc gatcgcgaca aagacggctg
    26341 ggatggggcg acggactgtg acgatgccaa cgccgacgta catccctttg cgccagagat
    26401 gtgcaacggt cgcgatgacg actgcgacgg actgatcgac gaacagaacc ccgattcgaa
    26461 cggtgcgcgg ctgatccaga ccggaaccga tggatcaaag cggacgactt cgtgtgcgga
    26521 ttcgacggtg ggactgtgcg gtgccaaaga ctctggcggg ttttatagtg gccgctgtgt
    26581 ctgtacctcg acactgcctt cgtcgacggt cgatcccaaa gccgtcgcgc tctgcccggc
    26641 ccaggacaac agtgcgggat ttaccgcgcg ctgcttcggt gccaaccagc ccaagccgca
    26701 gacttgtgat gcgctaaatc ccgttgatga tgactgtaac ggcagcctcg acgacgccac
    26761 cggagcgttt ttggccgaaa agggcaagac gtgtggcttg accgtgactg gaacgcgctg
    26821 caaagccggt acggttgttg gctgcgtgcg cagcaaggaa aatccgttct gggcgttgcg
    26881 ggttccgggc tttgccgaaa cggacaaata cctggtctgt accagccaga ccgatccgat
    26941 tcccgagcag tgcaacggct tcgacgacga ctgtagcggg gcgctgccgc ccaacgaaca
    27001 agacctcgat ggcgacaagt acatgtcgtg ttcgggatgt cagaacaccg acaatccggc
    27061 gctgtttaac gccagcatgc tgtactgcaa cgactgcgcc gacaacaacc gcaacgtctg
    27121 gccgacgatt ccgcccccga gcatgcaggc gcgcgacgga gcccctgagc tgtgcgatgg
    27181 gctcgacaac aagtgcgcaa gcggcaacgt cagcatgacg ccagccaacg acggtttcga
    27241 gcagtgcgga agcggaaccg atgccggcaa aggaacctgc tgcccgctgc tcaagctgtg
    27301 catcgatcca cagacggact tccagcactg cggtggctgc actggcgcgt gcagcagcac
    27361 cacggcggat cgctgcggcg gtggccagtg catgtgcaat aacgaagtcg cgtgcgatcc
    27421 cgccagccag accaagtcgc tgtgcaaagc cggggctggc tgcgtgcagt gtctttcggg
    27481 aacggactgc acgcgcatcc tgggttcggg aacgctgacc aaagcttgca tgccggcgca
    27541 gaatcgctgc gttgagtgca ccgacaactc gttttgcacc aacgctcttt tccctggcaa
    27601 gcaggcctgc gacaccgtgc ttggcaagtg cgcggtctgt ctggccgaca ccgattgcaa
    27661 ggccccggcg cccggctgca aagtaaaccc cggcgacagc agcaagaaca tctgcgtcga
    27721 gtgcacccag aacagccact gcaccgattc gagcaagccg acctgcgact tgacgctcaa
    27781 caagtgcgtg ccctgcctgg tcgccgccga ctgcaaagcc acgccgtcga actgcaagac
    27841 caatgcagat tcgacactga accagtgcgt cgagtgtctg tccagcaatg actgcaagac
    27901 gctgacgagc aagccgatct gcgacaccac gaaaaacaag tgcgtgccgt gccttgccga
    27961 taccgactgc ccggccaccg cgtcgaagtg cctgaaggat gcgggcggtg acaacaccaa
    28021 aaaccagtgc gtgtcgtgtc ttagcggcac ggactgttcc ggtgtcgttg gcaagccgct
    28081 gtgcgatctg tcgaaccacc agtgcgtggc ctgcaaagcc aacatggatt gttcggctgt
    28141 agtgaacaat ccggcctgct acgtcaacgc gatggattcg accaagaaca tgtgcgtgcc
    28201 gtgtgtgctt ggttcggact gcccgatgtc gacgccgacg tgcaaagtga acgccgccga
    28261 ccccagcaag aacatgtgcc tggtctgtct ggccgacgcc aactgcacga ccaccaagcc
    28321 gtcttgttac aaagaccctg gcatggacga caccaaaaac tactgcgtcg agtgccagac
    28381 caacgccaac tgcaagtcgc cgacctcgct gtgttacgtc gatgccgatc cgaagaaaaa
    28441 tgcctgcacc gtctgcctgg cgaacgccga ctgcaagagc ccgacggcta ccctgtgcta
    28501 tgtcgatgcg acggacaagc acctgaatgt ctgcacgcag tgcctggcga acgccgactg
    28561 caacggcgcc aacaagttct gccagacggt gacgggaatg ccggtcaaaa acctctgtgt
    28621 gccgtgcgcc gccaacgccg attgttcggg caagacaaac aagtgctacg tcgacatgac
    28681 gaccccggcg aacaccgctt gcgtcgagtg tttggccaac atggactgca caagtcccaa
    28741 gacgtgcgac atggccaccc acacctgcaa gtaatcctgc cagccggaat tcgccggctg
    28801 ttggccgccg cggagtgtct gtttagcggc agccaccgtc ggcggttccg ccatctcctt
    28861 ggccaccgtc cgcacccgta cagcccaggt ccgtgggagg cgctccgcaa taggggtcag
    28921 gacagccgta acaggccatg agtgtgacag cgaacgcgcc gccgccgagc gctcccaaaa
    28981 gaccaagtgc gcgcagtccc cacgacgaca ggctggactt gtccgcatcg cagtgcggac
    29041 agcttttgga atcgctgcgc acaaagcctt tgcacttgtt acaggtcgat agctgggcca
    29101 tgggtctttt tgctcctctg cgttttgttc gggctgaccg aacaatcaat aagaaaccca
    29161 gcgtaggcat ctttttttgc agtgtctagg attccgtggt gtagggtggc gcggtttggg
    29221 aatgtcgact cgtcgtctca gcgtgctatc gcaaattcgg ccagcgtatg tggtgtggga
    29281 actgacgctg cgctgtgatc agccgtgtac ccactgcgga tctcgggccg gggaaccgcg
    29341 tgagtccgaa ctaagtgaag cagaagcgct tgcggtggtg ccgcagcttt cggcgctcgg
    29401 ctgtgatgaa gtggtgctga tcggcggtga agcgtatctg catccggggt ttgtcgcgat
    29461 catccaggcg ctggcggcgg cgaacatccg tccaacgatg acgacgggcg gacgtggaat
    29521 caccgctgca ctggcaacgc agatggcgca ggccggcatg gccagtgtgt cggtgagcat
    29581 cgatggaacg cagcggaccc atgatcttct gcgggctgtg cgcggcggct ttgatgcagc
    29641 atgccaggcg gtcagacatc tgcgcagtgc ggggattgtg acgacggcca acacgaacgt
    29701 aaatcggctc aacctggggg aactcgaagc gctgtatgaa acgctgaaag agctgggaat
    29761 cgccgcgtgg caggtgcaga tcacaacgcc gctgggaagg gctgcggatc ggccagactt
    29821 gatcttgcag ccctatgact tgctggatct tttgccgcgg cttgcgctgc tcaaacagag
    29881 gggattttcc gacgggattc tgatgatgcc agggaacaac ctgggctact ttggaccgga
    29941 agaagcgctg ctgcgatcgc cccatccgca agggcgcgat cacttccagg gctgcttggc
    30001 cggacggttt gtgatgggaa tcgaatcgga tggcggagtg aaaggctgtc cgtctctcca
    30061 gagcgattcc tatgtgggcg gatcgctgaa acagagtccg ctttctgtcc tgtggaatga
    30121 atcgccccag cttgcattta cccgtcagag atcgacttca gagctgtggg gattttgtcg
    30181 cgactgcgac tttgccgaag tctgcatggg cggctgtagc ttcactgcgc acgcgctgtt
    30241 tggtcggatc ggaaacaatc cgtattgtca ctatcgcgcg cggactttgg ccaaacaggg
    30301 tctgcgcgag cggctgattc tgcgaaagtc ggcccccggt cttccgtttg atcatgcgct
    30361 gttcgatctg gtcgtcgagc cgctgacccg tccggaacct gtcttgccgc agacacagct
    30421 tgtgaaaatt cgtcgcccgg cctagcgaat taggggtcga aagacagacc cacttggcga
    30481 ttgcggcgct gccagaaatg ggaaacaagg cgtggccgct gcggcgtttg gacaggatgt
    30541 tcgcgcaggg aatcggcgac cgcttgggtc cacttggcaa gctgctgctt ggccacggaa
    30601 aaggcctgtt ccgacggggg aagagacgta aaccagtccg cttccagcgc atcccgttcg
    30661 gcctgaaaca gcgaaaggag tcgatctgga tgctgcatca ccatgcgatg cagtcgttca
    30721 tgctgccaaa acagcgaatg ggaatcggcc tgtccatgct gcggacgacc caaaaaggaa
    30781 gcgggcaggg gggcgaagac agacaccggt ttgaacagcg aagtacacgg agctgccgtc
    30841 gctgtagcaa agtgggaatc gcccgtttgc gcaagctttg aaatccacga tgcggtggtt
    30901 tgggaatcga ccgcgagacc gcccgcgtgc atgcacggac cgctcatccc accgagcagc
    30961 ggatgatagt caatcgtgcc gcgcgctctg gccacactgc cgtgatcgcg cagcaccgcc
    31021 gtcagatcga tgggctgggt ggcgtcttgg gctttctgtt cggtacgtgg tcggcgcaac
    31081 cgacagcgcg aggcccaagt cttcagccga tcgctgtgct gctcggcaaa accgggaatc
    31141 gtaagcccgt tggaaatgct gcgcacgccg cgcacttcct cgattgcgtg atgtggaccc
    31201 gccgtttcga gtacaaacgc ccgctgccgg tcggcgacaa ggaaactgga aaaatagcga
    31261 aagctctggt cgtggcgatc ggcactgccg ccttggccaa actgcgcaat caagtccaaa
    31321 atcaccgtca cggcacgttc tgcggtatcg gctctttcga cggaaagacg cagcagatcc
    31381 atcccggtta gaccgacgag tggcactgga tggcgggtaa agaccgcttc gtttccgacc
    31441 gtgacgccgt gttcgttggt cgccatttcg gcgccccaca tccacgatgg acgcgacagc
    31501 aacaccgcgt ttgtgtgacg gacctgcgga atccgcacat gggtacaggt gacgaagctg
    31561 ccgggtggat gaaacgcggc gggctgccag tccaagacct gcgcttcgcc cgacggcctg
    31621 tccgagtttt tggcaaacag aacgcaatcg ggttttacga cgacgagcgt gtcacacatg
    31681 gcgatctgtt ttccgatttg gccctggcgc gattatgctc gctgccctca tgaatcaaaa
    31741 gcttcgagcg atggtccttg gtgccacggg tgtggctggt caacagttcg tcgctgcact
    31801 ggcgggacat ccctggtttt cgcttgcgcg gctgtgtgcg tccgagcgat cggcaggtcg
    31861 cagctacaaa gatgcactgc gcgattcacg cggcagcctg cgcttttacg ctggtggcga
    31921 gccgcccgcc gaatcgctcg acctgccggt gtttgatagc aaagaggtat ccaccgacgg
    31981 aatcgatctg gtgttttcgg ctgtcgaaag cgatgccgcc aaagaactgg agccgcgatt
    32041 tgccgcccac gttccggtgt tttcgacggc gagtgcgttc cgttacgaac ccgatgtgcc
    32101 gattctgctg ccgggcgtca atcgatcgca cgcggcgctt ttgcggaccc aacaaaagcg
    32161 gcggggtttt cgcggcttca tttcgcccgg tcccaactgc accaccgtcg gacttgctat
    32221 cacgctggcg ccgatccatg cgcagtttgg tgtgcgtcgc gtggtgatga cgtcgctgca
    32281 agcggtgtcg ggggctgggc gctcgccggg ggtggttggg ctcgacatca tcgacaacgt
    32341 cattccgttt atcgcgggtg aagaagaaaa agtcgaaaag gaaacccgca agatcctggg
    32401 gcactgcggc ggggcgtcag ctgaatcgca tgcgatcgaa ccgtcaccga tcgccgtaag
    32461 ctgcacatgc actcgcgtcg cggttctcga tgggcatacc gaagccgtgt ttgtggaaac
    32521 cgagcgccca gcgccgcttg tggaagtcaa agccgcgctg ctcgattttg gtcgcgatct
    32581 ggcggcgctc aagcttccga cgatgccggc gcagctgatt tcagtgaccg acgatccata
    32641 ccggccccag ccgcggctcg atcgcgactg cggaggcgga ctgacgacgg tggtgggacg
    32701 cctgcgcgaa gaacgggcgc ttggtgcgca cggcgtaaag tacgtgctgc tgtcgcacaa
    32761 caccaagatg ggtgctgcgc aaggcgcgat actgaccgcc gagcagctgc tggccgatgg
    32821 ctatttcggc tagctggcgg cgccgcgttg atcccaaggt atccgtcgca cctacccgga
    32881 ttgggcagca atgagcccaa acaacgggtt tcgcattgac tttattcccc agtccttgtg
    32941 gaatgcgtca ttagtacact gttgtgcgaa atgtcgcttc cgtcaggcct ctctcgatgc
    33001 aaacgcggtg tttgtggcgc tttttccgcc acggcgcttg ggctgcatgc acgcctggaa
    33061 gcgacggttc agacgacagt gttgggtttg gtgccggggc cttacttccc ggtcgggccg
    33121 aatgaaggag cttcgattcg atgaggaagc agcagaagaa gtcgttgttc ttgggtgccg
    33181 ccgcaagcct tgcgcttctg ggggcttgta atgactacct gacgggtcaa gatggtgaac
    33241 cgacggggcc gctcaaggtg ctacggctga ccttgatcga cttcagcgcg aacaccggtc
    33301 atttggtgtt taccgacacc agtgttccgt cggactgcac gctgccgcag tacaaggacc
    33361 tgaagcagtg cacgaacgat cccttcatgg acacctacgg tgtcaagaag agcccgccga
    33421 accccgactc gctgcgtcgt ctgcgggtgg tgttcaacaa ggcgccgctg ctcgatctcg
    33481 acgcaactaa ccgtggcttt aacaccaccg agctggagct ggttccggac tcgggcgtgc
    33541 cgacggatct gaagctggtc gaccagaagg tcatcagcct cgcttgcacc aactgctcga
    33601 cggacccgac gctcggcgcg ggtattccgc tgacctacaa cagcttgcag cttaccggct
    33661 cggatctgag cccggacccg tcgcagttcc cgtatggacc gggactccag atggaagttc
    33721 tgaccagctg cacaagcacc accaacaagt tcaagtgcga caagatcggc ctgaccaaga
    33781 cgacggtcga tccgctggcc gcgctggagc cggacgcgag ctacaccgtt cagctgtcga
    33841 agggtctgtc gggccgcgac aaaaatcccg tgctcatgga cgatcgcgcg atggcacttt
    33901 tgtcgtttag aaccgagcca ttccagacga actccgtcgg aaatggcgac ggtgcaagcg
    33961 atgccgaagt tcgcccaggg atgagcggca gtgggacggc ttcagacccg tacatggttg
    34021 ccgatagcct ggtcgcgctc gacggtgcga tttcgattgc gctcaacgcg gcagtggacc
    34081 cgagccgcct gaacggcacg tcagcgactg cgaccgtgag cgtaaatggc ggggcggcga
    34141 cgccggttcc gctggtggtc agtccaaatg ccggtggcga ttgtgggaat cttcgctcga
    34201 tctacgtggc ccctgcgggt ggtgcggcgt gggcgactcc gtcgaagccg gacacggatg
    34261 acgtggtggt cactatcacg ctcaagggcg ctgcgatcta cgacctgagc cagatcaagg
    34321 gccatccgct cggcaaaggc atccacacga tgggcaaaga catcgtcatt cgcgccaagc
    34381 tgctgaaggg cgcgacgccg atggactacg cgggtgtccc gtcgagtgct gtcgaagatc
    34441 ccaacaactg ctagtacgac tcgccaggag tttgaacgat gcgacgaaag aaatcgaaaa
    34501 ccccagggaa ggctgccgta gtgttgcgcg ccaaactgct tccctggatg ctggtgctgc
    34561 ccggcgccta tctggcaccg caggtcgcgc tggcccagca gacgctggcc cagattcagg
    34621 gcaccgtcaa agacgaaaat ggcaagccac tggcgggtgt taccgtcgtc gtgcagagtc
    34681 cggctctgca aggcgagcag gcggaaatca ccgacaacaa tggccgctac atcatcaccc
    34741 agcttccgtc gggcgatgat tacaaggtca gctttttctt cggtgccgac gacaagccgc
    34801 gcgtggagcg tcctggcatt cgtctgagcc ttggcaagac cgtcaccgtc aatgctgaga
    34861 tcaacctcag ctcgacgaag cgcgaagtca aagtcatccg cgagtcggct ccagtcgtcg
    34921 ataccgcaag cgcggcgacg ggcgtggaag tcaaccagga agttctgcgc gcgacgccgg
    34981 ttcgtggccg taccttcgag tcggtcctga cgctggcacc gggtacttcg gatgtcgccg
    35041 gcaagggcgg tgccgcaggt ggtgacgtcg gtgtgcaaat ctcgggctcg acgggcaacg
    35101 agaacaacta catcatcgac ggtctcaaca cgtcagaccc gaacttgggc ctgattggta
    35161 ccgaactttc ccagtacttc atccaagaaa tcaacgtcat caccggtggt taccaggccg
    35221 aatacggtcg cgccaccggc ggtgtcgtca acatcgtcac caagagcggc ggcaacgagt
    35281 tcaagggctc ggtgttcggc tcgtttcagc cgttccagct ccgtccgcaa ggcgtggccc
    35341 gacttggcga agcgctgatc acccgcggtc ggacgaacct gctgtacgac ttcggcttcg
    35401 agctcggcgg tccgatcgtc aaggaccgga tttggttcta cttgggcttt gcgccgacgt
    35461 tcaccgaaaa cgtcacgcag cggcaagcgc gggcgctgtc gttcgacatg acgacgggca
    35521 aagcggcgat cgatcccgat ttccagtgcc cgacctacct gtcaaacacg cttctgtgcg
    35581 atggtccgcg cacgctcgcg ctgcgcaccg acgaaatcaa ctacgcgcag gaagtgaaca
    35641 gcatcaaccg gctgttcaac ggcatcgcca agctgcagtt caacctgaac ccggatcaca
    35701 acatccagct gacgtacatc gcgtcgccgc ggaccgggaa cttctacggt gcgacccgcg
    35761 cagtcgattt gtcgtcgctg cgtgcttcga acaccgatca ggttcacgac ggaatcttcc
    35821 actacatcgg caagctgttt gatcgcaagc tccagctcga cgtgctgtac ggctaccact
    35881 accagggcag ccgcaccgat cccgagcagc cgacgatccc gagcatttcg tgggcagcgg
    35941 cgcagaccga tccgttcacg ctcgccgact tcgaagacgt gggcgcgtgc cgtcgccaga
    36001 tgcggaccga tgcgatgggc cgctcggtgc tgtttaaccc gtgcccgctc actggttatg
    36061 cccgcggtta cggtcagtac agcgagagcc tgctccagcg gcatcagctc atcgcttcgg
    36121 cgacgtactt cctgcacttg acctcggcgt ggaacccgtt ccgcggcatt cacgccatca
    36181 aagtcggctt cgagttcgag tacctcgaca gctacaacaa ccgcacgtac accggaccgg
    36241 atctcgatcc gaacgatccg gcgagcggtc accgcgcgta cagccagcag cccgacggaa
    36301 cgattcagat ttcgcgcgag tacgcgaagc agaacctcga gaccaagcag atcgagcacc
    36361 tcaacaactt cgttgggcga tcgtatacgc agaactttgc ggcgtatctg cgtgactcgt
    36421 ggaacattgg ctgggcgccg ggtctggtgc taaacgccgg tgttcgctgg gaaggccagg
    36481 aactgtacgg ttccgacggc aagcgctacg tcgacatcaa ggacaactgg gctccgcgta
    36541 tcggcgccgt ctacgacttt acgcagctta cgtcgcggcc cggtcggtcg aaggtgttct
    36601 tcaactacgg tcgcttctac cagtcgattc cactcgacat caacgaccgt cagctcaccg
    36661 ccgagggtat gtactcgtcg ggcttcgtgc cgggcgccaa ctgcgacaaa gtgacgctcc
    36721 agccgggcgg tcgcgccgtg ccggttccgt cggcgggttg tcagttcctg gctgccgatg
    36781 gtggcgtcgt caacggcggt cgtgcaccgc tcatcgcgcc gagcatcaag ggccaataca
    36841 tcaacgaaat cacgttcggc gtaaactacg acgtcggcct ggatatcgtc cttggcgtaa
    36901 gctacatcca ccgcgacctc ggcaacatcg tcgaagacat gtcgccagac ggtggcacga
    36961 cctacctgct cgccaacccc ggtgtggccg ccgatccgaa cgtggtcgcg cagctccagt
    37021 ccgaagtgga tcgtctgaag ccggcaggca ccgcggccaa cgccaaggtc gaagatcagc
    37081 tcgcgtatca agatgccgtg tcgctgctcg gcgcgtacaa gctggttggc tcggtgttcc
    37141 cgaaagcccg ccgcaactac gacgccctgg tgctgaccct caacaagcgg ctgtcgaacc
    37201 ggttctcgat catctcgagc tacacctact cgcgcacgat cggcaactac cccggtacgt
    37261 tctcgtcgtc gaacggccag ctcgacccga acatctcgtc gcagtttgac cttatcgacc
    37321 ttctggcgaa ccgcaacggc ccgctgccca ccgaccgccc gcacaacttc aaggcgacgg
    37381 cgttctatga ccagccgctg tttggtgaca agggcaagct gacgatcggt ctgaccttca
    37441 cggcgacgag cggtcgtccg atcgaagtgc tcggtcggca cccgtactat ggtcgtcgcg
    37501 aagtctacat cctgccgcgt ggatcgggtg gtcgcacccc gatggtgacg cagttcgact
    37561 tgcacgtcgg ctacgatcac aagctgaccc agacggtggg catgaacctg tttatcgacg
    37621 tggtcaacct gttcaaccag caggaagtca ccaacgtcga cgacgagttc accgccacca
    37681 cggtgtcgcc gatcgtcaac ggccagctga ccgacctcaa gcacctgaag gcaaacaacg
    37741 gtacgctgcc gacgttcaac tcgaactacg gtcagccgac gtcgtaccag ctgccgctgt
    37801 acatgcggtt cggctggcgc ttgaccttct aatcccgcgc tcttttccgc tcgggccgct
    37861 cggcccactc tctcttttac gcttccccac ttgcttgcct gacaccccgg tttggggtgt
    37921 tgtgcgcgca tcgtttgcgc taggtttcgc gcatgaaaat agtgcgaatc gaccatgtgg
    37981 cgctgtgtgt gcccgagctt gaccctgcgc tggcggcctg gcagtcggtg ctgccgcttc
    38041 ttctggggcc gcgcgaatac gttccgacgc agcttaccga agccgccttt ttgctgacgc
    38101 ccgccgaagc cgatgcttgc gtcgagctga ttgcacccaa gccgcgcggt gaaaatgctg
    38161 ggctggaaaa gtttctcgaa aagcggggcg gtctgcacca catcgcattt gcggtggaag
    38221 acatcgccgg ggcgcttgtc gagctggcag agcgcggggt tccgcttatc gacaaagtgc
    38281 cacggccagg cgctcgtggg catcaggtcg cgtttttaca tcccaaagca ctgggcggcg
    38341 tgctcgtcga gctggtgagt gcccactaga ttgtgttgat atcgcctgat tggcacattg
    38401 ccaaattggc aaacctgcac agtctggctt gtgaaccaat caagtggctg ggcggcaatt
    38461 agactgaaag accgaacgag tcgtcttgat acaagcggtc aagctgcgcg gcgtatttct
    38521 gttcgacctc gcggcggcgc actttcatcg tcggggtcat ttcgccgtgt tcttggctca
    38581 gatcgcgcgg cagaagcagc agcttgcgca cttgctcgac gtgggcgagt cgctgattgc
    38641 ccgcccgaac cgccgacagg atgcgcgcgt gaaaatcggg atgcgcgaca acgggcgcca
    38701 tcgctcgctc gagctggtcg ctgcgtgccg acgggttttc gataagctcc cggctgcgtg
    38761 ctttgacttc gctttcgctg accaagccgc gccgcaggcc aaaatccagc gtttcgagcg
    38821 gactcggcgc aatgattgca ctcacgaacg accgacggtc accgtgcgcc aggatgtggc
    38881 tgatgagcgg atctgtgccc cggatcgccg actcgatgtt gcccggcgac aggtttttgc
    38941 cacccgcggt gatgattagg tgttttttgc ggtcggtgat ccgcaggtag ccgtcttcat
    39001 cgaggctgcc aatgtcaccc gtgtgcagcc aaccgtcgac gatggtgctg gcggttgctt
    39061 cgggattgcg gaagtagcct ttgaacaccc acggaccgcg cagcaggatt tcgccgtcgt
    39121 cggctatgcg ctgttccaaa ccagggatca gcttgccgac tgtcccgagc ttgaagttct
    39181 ggagcgtatt gaagtgcgtc accgtcgtcg cttcggtcat tccgtagact tccagaagcg
    39241 gcagtccggc ggcccagaaa aactcgagca catctttggg cgttggcgcg gctccgacga
    39301 tgaaatagcg cacccgaccg ccaaaaaagc cgcggatttt gctccacagc agacggtcgg
    39361 ccagctcgtg ggcaacctga atcggcagcg gtactttttc gccgcgatga agcagacgca
    39421 tgcgcgcaat gctcgtctca cgcgcccagg caaatagctt gcgcaccgcc agcggcctct
    39481 tttccagctc gctttggatt ttgccgtagg ccttttcaaa aatgcgcggc accgatccga
    39541 acaccgtcgg gctgacttcg gaaagctcat cgagcaggcg gctcatgctg ctggcataag
    39601 cggtcgccac acccgacgcg agccgcccat aacacgacag cacccgttcc gccgagtgcg
    39661 ccatcggcaa aaacgacatg gtgacgtcgt cggcgaaaaa gtcagtgaag tagtcgccgg
    39721 cttggaacat cgacaagatg ttgcggtgac tgaccatcgc gcctttgggc ggtcccgtcg
    39781 tccccgatgt gtaaatcaaa agcgcggtgt cttcgggcat gatggcgtcg agatgctccg
    39841 cgacttcctc tcgactgaga ggatcatgct caagcaccgt cggtgaacac agccgcggat
    39901 cgagctgttt gcggcgg
//
LOCUS       DAORGN010000018        39669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_352_length_39669_cov_6.286313, whole genome shotgun sequence.
ACCESSION   DAORGN010000018 DAORGN010000000
VERSION     DAORGN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 39669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39669
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_39669_cov_6.286313"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2825)
                     /gene="uvrA"
                     /locus_tag="PKL17_03705"
     CDS             complement(<1..2825)
                     /gene="uvrA"
                     /locus_tag="PKL17_03705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNK43862.1"
                     /translation="MPSTKPKQIAVAAAPSPIHEPPLRADSPLPPSVAEPDAIVIRGA
                     TEHNLQIDYLEVPKKRLVVFTGVSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGQ
                     LDKPKYESLRGLSPTIAIEQKTASSNPRSTVGTVTEIYDYLRVLWARVGTQHCPQCGK
                     RVAPMSAAEMANQLRRLPMGSQLTLLAPVAEGRKGSHSEALEKAKKLGYSRARIDGKI
                     VRIEDLPPLSKKHKHTIHIVTDRITLSDDAETDRRLADSLEGTLKLGDGMVLVQREGG
                     GLAAGETEEQLLSEKRACPTCGLAFPELSPQSFSFNTPLGMCPDCTGLGTRLEMDPQL
                     VIPDTRKSIRGGAIEPWATSMERGDGLVVSIVRSLSEEFGIDLDRPWRDLSREQQKLI
                     LYGAGDRRVEVKWEGRHGSGQWAMRFEGILNTLMRRYKQTVSEQMREYYEKFLRESHC
                     HACGGGRLRPESRAVYLLSSDDGPPKYSLPDVVGMTVGEAALLFDTLPLDGERALIAH
                     ELLKEIRARLGFLLSVGLDYLTLQRSAATLSGGEAQRIRLASQLGSELSGVLYVLDEP
                     SIGLHQRDNHRLIETLRRLRDQGNSVLIVEHDADTMRAADWVVDFGPGAGRYGGQVVF
                     SGTPQRLSQADTLTGEYLSGRRRIEVPKSRRPPQGFLEILGAREHNLKNVSVKFPLGT
                     LTAITGVSGAGKSSLINGILRPALLQELHDSRAPIGEHDRIVGLDQLDKLIAIDQQPI
                     GRTPRSNPATYTKAFDHIRNLFAELPEARTYGYKPGRFSFNVKGGRCEACEGDGLKRV
                     EMHFLADVFVPCEVCKGRRYNDATLRVRFHDKSIADVLDLPIVEARELFFHHPQLRPI
                     LDTLCDVGLGYLSLGQPATTLSGGEAQRVKLAKELCRRDTGRTLYLLDEPTTGLHFED
                     INRLLGVLHRLVEAGNSVLVIEHNLDV"
     gene            complement(2938..5277)
                     /locus_tag="PKL17_03710"
     CDS             complement(2938..5277)
                     /locus_tag="PKL17_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNK43863.1"
                     /translation="MSLRFRRLFLQTLLGGAGLLGILHAGLAAEEKTPTAANPSAASN
                     AATAGAANKDGGTRSPSATDSAATSTANGPDGGTHADLSVLDGGQDASVDLTPPPPPI
                     DPDVKAKLEALEAQNDLFLDQIGNRQTVLKNAIITVGNMLAGGASPTPASVKELFRAT
                     GEMVVSQILKHEGSVYSVHYSVDGKRLVTAVSDGSARVYDTANMQLVQTLKGHSQEVL
                     HAAFSTDAKRVVTASSDRTARIWDVATGKSLVTLTGHNQEINVAAFSRDGNRVVTAGS
                     DNQARIWDARTGRSMVTLSGHDGPVLYAGFSPDGSRVVTGSADDTARIWDAFSGRCIF
                     ILTGHSEDVTSAIFSPNGSRVLTAGRDKKAIIWDAATGRTHLNIFDAEDGVRTAAYSA
                     DGLRVVTTTTNRIARVWDAISGRALYVLKGHTQDLRGAVFSPDMARVATASSDGNVRV
                     WDVRSGRYGQIFQSKFELNSAMFSPDGGRVVTTHIDHNALLWNAKSERSQALLAGHTA
                     EVNWGAFSPNGQRIATASSDKTVKLWDGMTGRPVGTISGHQGPVFQVAYAPDGTRLCT
                     ASADRTAKLWNTQTLQPVSTLSGHTSEVRSCSFSPDGSRLVTASSDGNARVWDLTTGQ
                     TLLTLQGHNQDVLAATYSPGGARIATASADGNVMVWDARTGQAQWNLQGHNTVVRSVA
                     FSPDSSLLASSGDDHMARIWEVKTGKLLLTLEDKAGGVRSVQFTPDGTRVILAGGDGT
                     ARILLASVDGLLGFGCRVLRGTDLYPEVTTYCDRFPDAK"
     gene            5386..6474
                     /locus_tag="PKL17_03715"
     CDS             5386..6474
                     /locus_tag="PKL17_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43864.1"
                     /translation="MSAFSSRLRIVSLLFVPLLAGPSCTPYERRSGSYSAGAVDPIHF
                     PAPYLGEGGDGKRPGSGRFQYVTAYARKMPVSYYPLRLSGEQAAASDPLDVTNVPLPL
                     GYQFDPDRSPDGQDSRRCVSPPGYVYDQEARRQNAVPLDRQGNIFTALPVESDPPGKT
                     TYVPVVREVVVQSDKNPCQDPKSEDNLTHRTDLAIDLVPPPAGAVGAIPTGKPSGRYL
                     LWIVIDPAAEVLFPGGKLNSVTQLGPQRFGWFNQYLTAYLDGGVVPLDVLTPPNKVRL
                     KTQRLLYPQRVLDAKSGKVVMGELGMGFDLVEFRRGEEGYSPLCHVYSFEPNDPMMGF
                     EQSIAEVNPARIVDTGEVVYCPQPSEAR"
     gene            6478..9273
                     /locus_tag="PKL17_03720"
     CDS             6478..9273
                     /locus_tag="PKL17_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNK43865.1"
                     /translation="MRRIFSSLAPLVLLVPVSALAIGELDGRLAGVITDKVSGAPLPG
                     ARIRVSGSRLIGGPRTVATDDDGSYQIGALPPGSYDVELSYEGAKPLRRKIVIRQGES
                     FPLSIAWSIEELSESTKVIVEERKLTHPDSTQTGTVLTSDQTARVATSRRYQDVAQQV
                     AGVSGGSNPNIKGAMDSHNRYLVDGLDITDPVTNTFSANINFDSIESIEVMTGGAEAQ
                     YNSLGGTINLITAAGGNEWKISASQFFNHQSFSVGQQFGTQVYQGARPFLNLARPPQA
                     GYQTTVMVGGPVIKNKLWVNLSFEYLRRESSQPLGPTLGIQSPSSLSDRFLARLKLTY
                     APTAKHRITLSLSADPAFFNNVDQDNLRLGIAEDYQRQGGVFSILQWDYFHSDSVNTN
                     LQAGVQYSTVDVGPQGFFGSVDTSAYRGSGRFSSANDSYDPDRPQHYNADDGTYWYQG
                     GGISYDKRITFQFDPSVSVRGKLLGKHEAKFGIQSRVTYHSFDASTPGGQSFSDEGGG
                     PGESGLCLQQMGQTQGCNLLTFSPAFRNQQLGFSVGAFAQDRWQITQFLRVNPGIRVD
                     YGRTTNSRGEEVSSLIGFGPRLGLLFDLTQDGKTLFTAYYGRSNEVLSLLAAAYADIT
                     PSSLTQKWDAEAKTFRDFRSSGGDGGYKLDPTGTPPHTDEVTLSLRREVLENSVASID
                     YTYKRVGNIWDSIEVNQIWDPTGTRVVDYVDGVPHQVFRYSRPDENWRVYQGVDFVFE
                     SRPRQEWDLFVAYTLSFLYGPGAEQFAQVSGSQTLSQFYNPRMYGFYDGFLPEDRRHN
                     LKLRASYTFKGLLFGAFLNFQSGAPRTKRFYNQNEGDYTNRRSPSGTDPGQNPNDVTS
                     IAEVRTPALLTTDLRIAYDLHSLLQKVHLSVSVDLFNLFNLDAPTEIEARDVPSYGQV
                     RARQQPLRLQLGLQLQY"
     gene            complement(9441..9887)
                     /locus_tag="PKL17_03725"
     CDS             complement(9441..9887)
                     /locus_tag="PKL17_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNK43866.1"
                     /translation="MTPSLAKLYQEIILAHHRAPHHFGPLPAATHRAKLSNPLCGDEV
                     TISAVVDGDVVSVCHFEGHGCALCRASASLLTDAIQGQNRHAIEHMSKLLFAFLQDSA
                     DSERDDQARADLGDLVALSGVREYPSRRRCATLPWEALHKALQASD"
     gene            complement(9884..11122)
                     /locus_tag="PKL17_03730"
     CDS             complement(9884..11122)
                     /locus_tag="PKL17_03730"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNK43867.1"
                     /translation="MSVSVPPALLAARRRADFPALKKTVHGKPLCYLDNASTSQKPQV
                     VLDRLLSAYVDECGNVHRGVHLLSQQATRDFESVRDKVRRFLSAPSDSHVVFARGTTE
                     AINLLAQTHGFATVQPGDEVIVTQLEHHSNFVPWQRLCEQKQAKLVVAPITSSGTLDM
                     SALSSLISPRTRILACSHASNVNGAIVDVKAVCALAKQVSAITVVDGAQAVPHLPVDV
                     SELGCDFYCFSAHKLYAPFGVGVLWGKKDAMAALSPWQTGGGMVQSVTAEHTEFQPVP
                     FRLEAGTPAVAEVIALGTAIDYLAEIGMSELAEYESFLMLHMVQKLSQIPGLSALLPD
                     GPTVPVLSFQLSGIHPHDVGTALDLEGIAVRAGLHCAEPLFAALGIRGSVRASLSFYN
                     TVEEIDHLAAALIRTTELFR"
     gene            11249..12268
                     /locus_tag="PKL17_03735"
     CDS             11249..12268
                     /locus_tag="PKL17_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNK43868.1"
                     /translation="MPHESASLFAQGLRWQSSGHRVAVASVVKTWGSSPRPPGSLLII
                     CDSGEFVGSVSGGCIEGAVIAAAQNTLQDGVPQLLSFGVTQEMAWEVGLACGGRVEVF
                     VERAEADTLQTIAQALDARKPIVWVTALPSGRRWLFAGGDLADCPAAVRDLATAALGT
                     DEATEATLDDGQRFFLLPFSPALRLLIFGAVHTAQPLCQMATACGFDVTVIDPRAAFA
                     TEARFPQVELVCEWPEPALQHVAIDRRTAVVTLTHDPKLDDPALVAALRSQAFYVGAL
                     GSGKTHAARLRRLSDLGFSSEQTARICGPVGLRISARSPAEIAVSILAQIISVLRSDG
                     SRSIP"
     gene            12265..13902
                     /locus_tag="PKL17_03740"
     CDS             12265..13902
                     /locus_tag="PKL17_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNK43869.1"
                     /translation="MKFGQLAAAVGLICAHTLRLPDGTVFKKGRRLSPPDVDLLLQCG
                     MSSLWAAQLEDGDLDEDQAAAAVAAAVAGPNTQVAEATTGRCNVHAACDGLLLVAAPA
                     VGRLNHLDPELTLATLPHLSPVRQGEMVATVKVIPFAVCGALVSAAQKLAAQGQTVDG
                     DMIRIAPFVPRRMGLVLTVTPGLPDSISDRAAAAQRVRAERLASHIVEERRCFHDEVA
                     VSTALRELVQAGCDPILVLGASAIVDRRDVIPSAIESAGGSVLQLGMPVDPGNLLLLG
                     KLNSATVIGVPGCARSLRRSGFDFVLERTCAGLPVTADALQDLGVGGLLHEVPWRPMP
                     REKALLPPQVAAVVLAAGRSQRMGKDNKLLALLDGKPLVCHVVDALAKTAVSPIVVVT
                     GHQSDAVRAALSGRDVRFAHNPDFADGLSTSLRVGLHQLDSTIDGALICLADMPRVRR
                     DHLEALVGAFEPEDGREVVVPTFAGRRGNPVLWSARHFAAMRELRGDQGARELLRRLA
                     AVTCMVPMPDDGVLLDVDTEESLAHIRRVPLDKSSGD"
     gene            complement(13899..14216)
                     /locus_tag="PKL17_03745"
     CDS             complement(13899..14216)
                     /locus_tag="PKL17_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNK43870.1"
                     /translation="MSWLFLGIGILFEVCGTTCMKLSNGFSRIVPSVVMLLCYMASLG
                     MLTLALKQIPMNVAYAIWAGMGTLLVVMLGLFFFHETLSLPKLASILLIILGVVGVRL
                     SGG"
     gene            14408..14971
                     /locus_tag="PKL17_03750"
     CDS             14408..14971
                     /locus_tag="PKL17_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK43871.1"
                     /translation="MAVSNAFLTSHAVAELIGVSPSTVLSWIDKGLLPAFRTPGGHRR
                     VEPSVLVEFLRNHQMPVPRTLLPQARRMLLIDDDATFLRSTKRQLKQNFPDLEVEIAE
                     GPVEGLLKIGTDRPDAVLLDAMMPGMDGVEVCRQIRQNPATADISVIALTGRLDDLLE
                     ASFKKSGATACLRKPLDINQLTQLLGL"
     gene            14975..16462
                     /locus_tag="PKL17_03755"
     CDS             14975..16462
                     /locus_tag="PKL17_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43872.1"
                     /translation="MTVLAVAQEERASRSSQKLRALRHQASEFGVGPIACTAWHPSFQ
                     VLSGLVENLSMGGLLVRFPGVLKLGTMDVDDWLLSVHVSLRTGEVICHSDAVVRHVQT
                     DGDDLLIGLQIKKGVVDLPTLYERQTRYRFAARCDQAERVADASKILPQFKEWVSDLR
                     NYLERMKAFLDQEESALESEDLFTRDQIVAQYLGEVGPRIIQRVQTASHQMAGMLGNL
                     SDDEHAAHRAYAQHHLGELFLHAPFVRRAHKKPLGYAGDYEMMNMLYRPSHGEGCSLF
                     GRVLNMCTTSEVAARANINRISFLQDKIRATLRLHSASGKARIRIASVGSGPAKEIED
                     LLENEPHLGPLLDVMLIDQDVRAISYCERRLVPLAEKTGAQLHFVRESVRRLLCGGRL
                     GYTMGQRQLIYSAGLFDYLSDRSFVALLSALYEGAAEGGQILVGNVDQSNPTRYFMEY
                     FAEWFLIHRSRQQLLDKAKLLRPAPSQVRVESEPLGVNLFLVLQK"
     gene            16459..>18349
                     /locus_tag="PKL17_03760"
     CDS             16459..>18349
                     /locus_tag="PKL17_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK43873.1"
                     /translation="MTPDFKSYLQDHNTAAVRLTCILCAVLMPAGVFLDWFTNRQFVG
                     EFLVLRLLASLVSLLVLGLARLDSFRRHSYPLITIVILACTGAIEVMIVRLGASASSP
                     YYAGLNLCILGSGLVYVWSARQALLVCGLVLLMWLVPALASGPEIYGAFVNNLYFLVL
                     TSIIAVAANQMRYNLAHREWEAKTALSRTSAELQTSIERIQELDRLKNQFFTNISHEL
                     RTPLALITAPIEEMLSRPGLPDIHRNALQVVLRNGLRLHRLIDGLLDLARLEAGQLRL
                     RVANLDLPHLLRNVVGAFQPAAESMGIGLSFALPDEPLPTSLLGDSEKLEVVMTNLLG
                     NALKFTERGGSIAVNVRAVPDAVEIDIKDSGIGIPEGEQSYIFERFRRVESAGRKQGG
                     AGIGLALVKELVELHGGSVAVKSASGEGSTFTVRLRLGSEHLQDDLVETPSSPPSRPP
                     SELSRAASPHQALVHIDGALDPRLEPGIKGNSSQSIPVLVSGGGHSRAHILIAEDNVD
                     LRKFLQELLAPYYNVTAACDGKEALERLHERLPDLVLADVMMPQMSGIELCQQVKRDL
                     RTRTLPVILLTARSGIDETLEGFSHGADDYLVKPFSPRELLMRVAVQLKLRRLTAQVA
                     NAARLA"
     assembly_gap    18350..18449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18450..19214
                     /locus_tag="PKL17_03765"
     CDS             <18450..19214
                     /locus_tag="PKL17_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43874.1"
                     /translation="QLKLRRLTAQVANAARLAAVGTLAAGVAHEIKNPLNAINTGAAA
                     LRKRDGLDPSLQAEILDMIEECVGRISEITTALTEHARPADGEGLSLFDVQKGIETTL
                     RLLADRLRASSIQIVRDYRTERLIVARPRQLNQVFLNLLDNAVRASPASGRISLTLSE
                     TDRETVSLVVHDEGPGVPVENRERIFDPFFTTRAPGEGTGLGLYLSRQIIESHGGSLR
                     VGSVPGRGAAFIVELPVAGPTAALGGTSRSSEGAAA"
     gene            19211..20368
                     /locus_tag="PKL17_03770"
     CDS             19211..20368
                     /locus_tag="PKL17_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43875.1"
                     /translation="MKPSKPAPSGERRAVVLYVDDDRANLLAFRAIAEPTYEVVVAKS
                     GDEALQLIEQNPDIAVLMADQRMPGMSGIDLCERVQLSHPDIVRMLVTAYSDLQAAIA
                     AINRGHVSRYLHKPWNAEELLATIRDAVERYRLKVTVQELQVRISQSERLYALGVLTA
                     SIGHEIRTPLSIVSSNVQFARKALVDALKSPQLRSAGSGLQEVEAALIDAEEGAQRLI
                     DIADSILLSSRSEPGDREPVDLSHVLHSVLRLTRSEATHRARLLIAQSAKPRIYGSAT
                     RVGQVLLNLVLNALQALPDRPIERNEVKLSLDEKDGRAIVEVADNGIGIEPQNLAHIF
                     DPFFTTKKKGTGLGLAISKQIVEEMGGQIHVESRPGQGTRFRISWPLLAST"
     gene            complement(20372..21250)
                     /locus_tag="PKL17_03775"
     CDS             complement(20372..21250)
                     /locus_tag="PKL17_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43876.1"
                     /translation="MPSPQRAKTEQPNRSTHPAMLIRAVASRRPTSASAALATLLGRA
                     KDLHAEGIGPKLPVDRCLTALAPILKFVAAEPTLHGEVALSQEMCGAVLRIAEELQGL
                     TPLAPKLREPLSIEPPRSARHGDTVRTGALLLEAYRDAVRRGLRGKNQPGIKAEFGLG
                     NELTLTDGQKVAEGIARFLLAASQHPDVIAAAGLQPAQLLGLAAQERVLRALEADIRS
                     QATASESQYRRRVLHLAIEYFLDRFQAALHLHLPSKSSRLREGLALLPSQPAPVTPRS
                     GLTTCQVTESGRLIFA"
     gene            21339..21800
                     /locus_tag="PKL17_03780"
     CDS             21339..21800
                     /locus_tag="PKL17_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43877.1"
                     /translation="MMEAKVALAAFVPLRIGVGQFVLAQAMQQIAAGWLVQPLLTRHL
                     ESVQKGERGLSALSSVAAHVLAHVQLYSVATCTAQIVFGLLLLLGLFGRTAAGLLLFG
                     HLFAAVLLGPRLFDSATMLLCLGLVSLSLVPSGRVLGLDSVLASRLPSWLR"
     gene            complement(21804..22256)
                     /locus_tag="PKL17_03785"
     CDS             complement(21804..22256)
                     /locus_tag="PKL17_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43878.1"
                     /translation="MRLHRILPRLVAPVLLYLAVGCAHIEYVTPPRYEFSGVSISQIK
                     IEHDDDDLEISLLMTNQTDRFMVIDRNQILCALPSGQMLARSNAMVFGIPTAPTYTLP
                     PRASHEVQLSYKVSEKTPQVALMLNRGIFIDNRSVELPAYVITQKVDK"
     gene            22366..22764
                     /locus_tag="PKL17_03790"
     CDS             22366..22764
                     /locus_tag="PKL17_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43879.1"
                     /translation="MKKLQLAAAAVLCFVPLLVGCGKKGVDAEIQAFKDAGRTVSEFI
                     DADAGLMQAKRCRQGSIDQMAVLLCEYPSRDAASVGVRNAATWAGDCPTWLSLQRDKV
                     VFTVADRSEVDQNGKTISALSKVFRRLAKK"
     gene            22798..24084
                     /gene="nhaA"
                     /locus_tag="PKL17_03795"
     CDS             22798..24084
                     /gene="nhaA"
                     /locus_tag="PKL17_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNK43880.1"
                     /translation="MSSASPASGLAPRRLGQLIRGAFVRFIRAEAASGIILIVNTVLA
                     LWLANSALHGRVEQIWQLAFSIPGIGAPLSLRELVSDGLMALFFLVVGLEIKRELIDG
                     ELASVRRAALPVLGALGGMLAPALIYLAFTGGSPFARGFGIPTATDIAFTVGVMTLLG
                     RCVPLGAKVFVTALAIADDLGAVVVIAIFYSHFGSLLSMLPVIGLTLLLMGLHLLSVR
                     RLVVYLPIGALLWLAMHRAGVHATLSGVVLAMAIPYREGQGTSASSVSPLHRLEQTLH
                     PVVAFGILPLFALANAGVLLPPPALAWQVFHSQPVTLGVFFGLLLGKPIGIFACSVLA
                     IRLGIGTLPSHTTYRVLFGSAMLAGIGFTMSIFITGLAFSDASVVAAAKTGVLAGSVC
                     SALLGSLFLRFVSRANPAESVPRDETVSDSPSDRSA"
     gene            complement(24081..25847)
                     /locus_tag="PKL17_03800"
     CDS             complement(24081..25847)
                     /locus_tag="PKL17_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43881.1"
                     /translation="MKSYFTESAIDTETSIRLLDRFFSVFAVSFLILLFIGVPFLFVR
                     KLASGLTTVIVLGFMMFTWRISRRGAPKKALRWFAGLSWIAAVVTLYLGMQPTIMVFV
                     MAVAVVLAVVAGVRDGAIFAGSYLLAWLLYLVLSAKGMAPKVYFLGAGPIGWLHGLVS
                     ILIVLVPLPELVAKLQEAQRQAEAANRAKSSFLATMSHELRTPMNGIMGMSQLLLSSG
                     IPEEQRQQYVRAILSSGQTLLTLLNDVLDLSKVEAGKLALIPTKFSPATVIQDTFQLF
                     EIQAASKGVRLKATWNGPHCTYRGDAVRLRQMLVNLVSNAIKFTERGEVQVTGIEIQN
                     DAHTARIEFSVTDTGIGIAPTKQALLFQPFSQLDSTNTRQQSGTGLGLSIVRNLAHLM
                     GGEVGFSSEEGRGSRFFFRVPVEVLSAAEAEPVREPVGNAMLPTQNARADGKTAALVL
                     VVEDNMINRTVVEGFLKRQGVSVQSVENGVEALEVLTRQPDRAPPQLVLMDYQMPLMD
                     GVEATRRLRAWEREQNRPRLPVIALTAAAFDEDRQRCTEAGMDDFLSKPIHADQLTKL
                     LTKWLPKGSLRPPADPITSKKS"
     gene            complement(26024..28270)
                     /locus_tag="PKL17_03805"
     CDS             complement(26024..28270)
                     /locus_tag="PKL17_03805"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNK43882.1"
                     /translation="MVSKSHTASLRLRSEAWNMVYRFLRQWPVMLAVSALAVSGGLYY
                     AQRYRPTYQSQAQVRVGEELDAKAVPGQREDSSDLADLPETLANAFRARLVIDAQLKA
                     SVAALVKQPELAHLTELQTEKGQDELVENIKKTLLIEAISRRLFVMTYNARDPHLSQL
                     VLKTLSEEGVQGVIAQRIETAKRARMFLQDESERSRQRMLDAENQVVQFVRMHPKLLV
                     SISATDRSKLGLQAADKLLVTTRNRVLTPPTKMAESYSSPELRPLLEKRAQFEAQIAQ
                     IENAHKYDPTQQKMLEIERLQQQLLELKGQGYTADYPEYRRVSNDIDRIQRDIRDTRT
                     RKDPKLAEDLMVLGQARAQMAALDRQIMTIRRKLTGGDAKLSADEEALNAEAQYARLF
                     RDMESARQAYDKLREREMESVVSEQLIKMPGNAAARIIDPASFPNRPRGLSKKMMTAL
                     LALLGLVVGLLSGVARSLADKRIYSAIDLWHAARLPVLARVPSSDKRNRRNVLPEVLT
                     SGESGERHAAVGNPEPAADRKTGASRLVDLGYVVHPAPSTQPSADLVILSNPDGARAE
                     QYRLLCCRLQERGNPRLLMVVSSQPSEGKSTVAANLALAMSEGGGAKVAVIDAHLLGP
                     RLHKLMMPQSEPTPSDIRERKPEVWQVTPDLCLVPATGLGQRQNRAAVENSPAFAALL
                     KDLQAAFDYVIVDTPPLSLAADARLLLRHGGTALLVVRAARTDLEELGLALDRISRHA
                     ISGLVLNA"
     gene            complement(28488..28841)
                     /locus_tag="PKL17_03810"
     CDS             complement(28488..28841)
                     /locus_tag="PKL17_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNK43883.1"
                     /translation="MDELLRLTEFDFENLFALTGRDAGFVKMLLFQFYSGHEHLLQEI
                     RDKLQAEDYVGARQRIHRLRGGAGNLGAMRLYEDATVLEEEIHKTGTYRAESLSRFAE
                     SFEKVLLEISTLTPL"
     gene            complement(29072..29533)
                     /locus_tag="PKL17_03815"
     CDS             complement(29072..29533)
                     /locus_tag="PKL17_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43884.1"
                     /translation="METAEHEALCRRCGMSCHFAVPVNGLAVVLDELHCKFLGKASDG
                     RYHCTVYEDRYAKAPWCHSAESALSGGFLAQDCPYARGTPGYRGKVRLSASLLQKVKP
                     AIIAEIIRLGIPCGADPEAVLPLLPGPQGSWQYALSSDGQRYIFLNTLATP"
     gene            29619..30233
                     /locus_tag="PKL17_03820"
     CDS             29619..30233
                     /locus_tag="PKL17_03820"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNK43885.1"
                     /translation="MTHDQIDPAELTPRKMYRLLIGLVTPRPIAWVGTISPQGIPNLA
                     PFSFFNGVAANPPTLVFSVSTRRDGSHKDTLRNIMAIPEFTVSTVSYSHAEQMNATAA
                     ELPYNESEFSHAHIDPVPSVRVRPPRVAGAKVQMECVLHQLVPVGEGPLSATLVIGRI
                     VLMHLENGVLDPQGDPDPQVLDHIGRMGGDGYCRIGDRFSIKRP"
     gene            30399..31718
                     /locus_tag="PKL17_03825"
     CDS             30399..31718
                     /locus_tag="PKL17_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNK43886.1"
                     /translation="MKTTNLFFWVGILSAGLTASGSCNPNRAIQEYDTDGTMSEADMA
                     RPRPYDFALPCVGLACQKPKCGGSNSTTLSGTVFAPNGQDPLYNVLVFVPNGELKPFD
                     DGVACETCEKSVSGTPLTATLTDTKGRFTLKDMPAGANIPVVIQIGRFRRKITIPFVT
                     QCTDNTVMNGDARLPRNSSEGDIPRIAIVTSTYDPTECILNAIGIDANEFTAPSGSGR
                     IHIYHGSGNNISGAPAGNALWGDLPTLKKYQLVALPCSSYPSGADPGVQNLFDYANAG
                     GRLFVTDLSYPVISQGKGDWPSTGNFASPGSFSNPASIDTSFPKGKALSDWLEGFGAA
                     TGGSLSLSETFSRVGMIKPPAQRWLYSGGNTQSYTFNTPTSVAEKDQCGRVYYSSFHI
                     GSGRSTTGTFPSACHSKPLTPQERVLEFMLFDLTSCVQSDSVPPVIP"
     gene            31795..32892
                     /locus_tag="PKL17_03830"
     CDS             31795..32892
                     /locus_tag="PKL17_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNK43887.1"
                     /translation="MDSAPTNRLRSHREQAGLSQQELALAVGMSRQSVVSVESGRQSP
                     SVDVALRMADVLGCGVEELFGPAQPSGRLLAEPAFGSPDGRVALSQIGGRWVSYPLCG
                     SDIGRAADGIALKTRGKKTEIEPLRPSRLLQDNTVLLGCAPALGLLADRLNSRAGAGR
                     FLWFSRSSTEALTALGCGHSHLAGVHLTDEKTGEANLPDVRRHLLRKPIELVTLARWE
                     VGILTAAGNPKRIRSLAEVSRHRLVSRERGSGARRLLERELTRHGVSIKLAQQAPMMA
                     HGHLAVATAISMGAADVGIASRDAAQAYGLHFQPLTEERYDLVLPRSLLADLRMVRML
                     ELLHSASFQRELTALGYDARSCGQRIVELVT"
     gene            32889..34085
                     /locus_tag="PKL17_03835"
     CDS             32889..34085
                     /locus_tag="PKL17_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43888.1"
                     /translation="MTKHCACWGSIALLLGVLLFARNADAQPACDDSVNLPNPVYLTV
                     GDTQANLMRELGAKLRATEQITLIWRATGSCTNLDTLYGDVKMSGNFSYIPAGYDPQT
                     TPNPPVCTVAAPGVLADIANSIVFLDTCPTPKPTDVMDALGPVQSFVFAVPRASSQTA
                     ITAEEAYFVFGFGSSGQVAPWLDNSLLFARPVTKGTVISIGASIGVPAGKWQGQKINL
                     SQDLASQLAAAPSPDRAIGILGSEVYDGLRSTLKALAFRAFFQRRAYFTDSTTASFDK
                     QNVRDGHYHMWSYTHWLLKLDGMGKPRKALAQRVVDVLVGNSVTPEPQFEPLEAPIRV
                     GLIPRCAMRVQRQTEGGDFSLYQPSEPCGCYFDFRATGTTKCSACTGSKMCGTGVCRH
                     GYCEER"
     gene            34088..34324
                     /locus_tag="PKL17_03840"
     CDS             34088..34324
                     /locus_tag="PKL17_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43889.1"
                     /translation="MHRLGFLCALLGCLVGCPEDTSVTNPAAAVDLAQSGPPCVSSPQ
                     THVEILNACTSAQSVDKKTMLPLLRADGTLPPLP"
     gene            34299..35657
                     /locus_tag="PKL17_03845"
     CDS             34299..35657
                     /locus_tag="PKL17_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43890.1"
                     /translation="MERFPLCRSLQRVLWPLLLLTVWDVSPAGAEEADLTERVRILEA
                     RLAAFESQTAATHFPWQKSDSFALRIGGFVQADAVAYRQDSEDQLDGATGQPLNETRF
                     VIRRARLRAEAEARYVGGALELDGNTVGTTQVRLLGAEVSAQFPSPGGDIPYVRATLG
                     LFKIPFGREVRQYDPERLFLERSQVVRALFPGEYDLGIQLHGGFRFLRYALALMNGEP
                     SGEAQFAARDPNQSKDLVGRISVDATWWKMVRLSAGMSGVYGEGFAPGRLAGKDALSF
                     RDDNDDGQVQPSEILVVRGQAAVPSQNFGREALGFDAEVTVQIPKLGALQLAGEVVIA
                     RNLDRGLFPSDPVASGRDLRELGYYAQMVQEITPYACLGVRYDVYDPDADRQQQLGVM
                     RIPVSPLFSTLTTTLAVQHPRYGRVAMEYQNQRNPLGRTAAGTPTTLGKDSVVLRAQV
                     KF"
     gene            35666..36556
                     /locus_tag="PKL17_03850"
     CDS             35666..36556
                     /locus_tag="PKL17_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43891.1"
                     /translation="MRALAQAFLGLWSLTGCDSASSKSAVTALLRVENAQRVEGVPPA
                     LDDVSYGPQVLAVTLLGSRIWPGEIGKPLSGTLAEGSQAVSLHIEGDSAHYVLPAGAA
                     DVTAKSLPTFSAQLSFSPLLPVGMTRLAVQAVDAHGQYGPPSHQALWAEPSDTPQGAL
                     VIALGWERAADLDLHVEQPDGQELWSRRKSGFSQPRPGRPPSAGSIGYLDQDSNSQCV
                     LDGQQREHVIYPVAPMPGRYRIRVDTFSLCAEVTAYWQVAVLFDGRLIASARGQSVAS
                     DTRGSHGQGSGTLALELDLR"
     assembly_gap    36571..36670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36671..38756
                     /locus_tag="PKL17_03855"
     CDS             <36671..38756
                     /locus_tag="PKL17_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK43892.1"
                     /translation="CGWWGFGAVCWVWVFWGVWLGLFSVAAAAPGDDSFSTVVRADKA
                     SAGSLEARVFTKDGQAIPGLFGDAVRAVEAQAGVSRPQLGSGQLIVWGAAPSETRILW
                     DGVELPALYHFGGLRTVLAKSTVDSLALQPAGYGSAYGRGLGGILLLSSSAIPQGIHG
                     EVSADFLDASAQLSAAFSSRLRMAVSGRIGYVDRLIPLLSSAPLADFYPLPRYWDAQG
                     RVVIKLRSLDDGQEQLSFRVLGSGDSRTRVQASPDAASAQTETWTRTFYRIAADYLRS
                     QSGQKIEVTPWFGIDEESWRALFGGVLSQLSRRDIRYGLRAVISQRPLRSVSFVVGAD
                     VLGDVAAISREGTLTRPPREGDIAVFGQSPGREVSSDSWSVHQGSFAVFSQITWRYSR
                     LQLVPGLRAVAYLSDVSRLLPRIGDTPPLGFRRIDFALEPRLQVRMQAASRLALHAAA
                     GLYHQSADAADLSAVFGNPQLGPARAVHGAVSAELQVFEPLSIEFGSFVRALFDLWAR
                     SPDLTPPLGRSLADSGSGLSYGGQLVVRLSPLRNLSGSVSYSMGRALRRSAANAPWRL
                     ADFDQPHNLQVALRYVLFGFGIGARLRYASGLPRTEVTGAYFDARDDRFDPLFGPHNA
                     IRLPDFFQIDAQIDRSFVLSARLRLSVLLDVQNLTNYQNAEELAYRYDYRERAYLRGM
                     PILANLGVQLTF"
     gene            38793..>39669
                     /locus_tag="PKL17_03860"
     CDS             38793..>39669
                     /locus_tag="PKL17_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43893.1"
                     /translation="MISACVPDLGPPQSLLDGPRMLAIRSEPAEVFPGDTVQLTLVAI
                     DAAGALVSTDVDWALCASPKPQVENNVVSADCLSDMVIPISGTTQSQAVTVPLDACAR
                     FGPELPPALPGMPAGRPRDPDDSGGYYQPVRVTALGMQAIGLVRIRCGLAAATPEVAV
                     QFRSNYVANRNPWATKLSIFDGDQMVSAQQLPASRSLRLRLVIPSESRESYLRFDPTS
                     QTLVQTAETLRASWLSAAGSFTLGQTFVTDGSDPSLYAETQWTAPSQSGPAMLWTVLR
                     DSRGGVWTQSYPVQIT"
BASE COUNT         7337 a        11291 c        12424 g         8417 t
ORIGIN      
        1 atgacatcca ggttgtgctc gatgacaagg acgctgtttc ccgcttcgac gagccgatgc
       61 agaacgccga gcagccggtt gatgtcttca aagtgaagtc ccgtcgtcgg ctcatcgaga
      121 aggtacagcg ttcgtccggt gtctcgtcgg cacagttctt tggcaagctt gacgcgctgc
      181 gcttcaccac ccgacagcgt cgttgccggc tgaccgagcg acagataacc cagtcccacg
      241 tcgcagagcg tgtcgaggat ggggcgtagc tgcggatgat ggaaaaacag ctcgcgcgct
      301 tcgacaatag gcagatcgag cacatcggcg atgctcttgt cgtggaagcg gacgcgcagg
      361 gtggcgtcgt tgtagcgacg gcctttgcag acctcgcagg ggacgaacac gtcggcaagg
      421 aagtgcattt cgacgcgctt gagtccgtcg ccttcgcacg cttcgcagcg cccccctttg
      481 acgttgaacg aaaaacgacc gggcttgtag ccgtaggtgc gtgcctcggg cagctcggca
      541 aacaggttgc ggatgtgatc gaacgccttg gtgtacgtcg ctggattgga gcgcggcgtc
      601 cgtccgatcg gctgctgatc gatggcgatg agcttgtcga gctgatcgag cccgacgatg
      661 cgatcgtgtt caccgatagg ggcgcggctg tcgtgcagtt cttgcaagag cgccggacgc
      721 aggattccgt ttatcagcga cgacttgccc gcacctgaca cgccggtgat cgccgtcagg
      781 gttccgagcg gaaatttgac cgagacgttc ttgaggttgt gctcgcgcgc acccagaatt
      841 tcgagaaaac cctgcggtgg tcgacgggat ttgggcactt cgatgcgacg gcgccccgac
      901 agatattcgc ccgtcagtgt atcggcttgg ctgagccgct gcggggttcc cgaaaacacc
      961 acctgaccgc cgtagcgacc cgctcccggc ccaaagtcga ccacccaatc ggcggcgcgc
     1021 atggtgtcgg cgtcgtgctc gacgatcagc accgaatttc cctgatcgcg cagacgccgc
     1081 agcgtttcga tcaggcggtg gttgtcgcgc tgatgcagcc caatcgacgg ttcgtcgagc
     1141 acgtagagca cacccgacag ttccgatcca agctgcgacg ccagccgaat ccgctgcgct
     1201 tcgccacccg acagtgtggc cgccgatcgc tgcagcgtca gatagtccaa accgacgctt
     1261 agcaaaaagc cgagtcgggc ccgaatttct ttgagcagtt cgtgggcaat cagcgcgcgt
     1321 tcaccatcga gcggcagcgt gtcaaacagc agcgccgctt ctccgacggt catgccgacg
     1381 acatcgggca ggctgtattt gggcggaccg tcgtcgctcg atagaaggta caccgcacgc
     1441 gactcgggcc gcagacgacc ccccccgcag gcgtggcagt ggctttcgcg caaaaacttt
     1501 tcgtaatact cgcgcatctg ctcgctgacg gtctgtttgt agcgacgcat cagcgtgttc
     1561 aagatgccct cgaagcgcat cgcccactga ccactgccgt gacggccttc ccacttgacc
     1621 tcgacgcggc gatcgccggc tccgtacagg atcagcttct gctgttcgcg actgagatcc
     1681 cgccagggac gatcgaggtc gatgccaaac tcttctgaca ggctgcgcac gatgctgacg
     1741 accaggccgt caccgcgttc catcgacgtc gcccacggtt caatcgcgcc gccgcggatc
     1801 gatttgcggg tgtcgggaat gaccagctgc gggtccattt ccagtcgggt gccaagaccg
     1861 gtgcagtcgg gacacatgcc gagcggcgtg ttgaatgaaa acgactgcgg cgatagctcg
     1921 gggaatgcca agccacacgt cggacaggct cgtttttccg acagaagctg ctcttctgtt
     1981 tcccccgcgg caagtccacc gccttcgcgc tgcaccagca ccatgccgtc accgagcttg
     2041 agcgtccctt caagcgaatc ggctagtcga cgatcggttt ctgcatcgtc gctgagcgtg
     2101 attcgatcgg tgacaatgtg gatggtgtgt ttgtgctttt tggacagcgg cggcagatcc
     2161 tcgatgcgga cgatttttcc gtcgatgcgt gcgcggctgt aacctagttt tttggctttt
     2221 tcgagcgctt cgctgtgcga acccttgcgg ccttcagcga cgggcgccag cagcgtaagc
     2281 tgacttccca tggggagccg tcgcagctga ttcgccattt ccgctgccga catcggtgcg
     2341 acgcgcttgc cacactgcgg acagtgctgg gtaccaaccc gcgcccacag cacccgcagg
     2401 taatcgtaaa tctctgtaac ggttcccacc gtcgagcggg gattcgatga cgcggtcttt
     2461 tgctcaatcg cgatcgtcgg agacaggccg cgcagcgatt cgtacttcgg tttgtcgagc
     2521 tgaccgagaa actgccgcgc ataggccgac agcgactcga cgtagcgacg ctgaccttcg
     2581 gcatagatgg tatcgaacgc cagcgacgac ttgcccgatc cagacacgcc ggtaaagacg
     2641 accagccgct ttttcggcac ttccaggtag tcgatctgta ggttgtgctc tgtggcgccg
     2701 cgaatcacga tcgcatcggg ttcagcgaca gacggcggca gcggggaatc tgcccgaagc
     2761 ggtggttcgt gaatgggcga tggggcagca gcgacggcga tttgcttcgg cttggtgctc
     2821 ggcatggcaa acactcgcct ggctttggcg ggccagttac cggcaagaag ctagctgcaa
     2881 cgggcgatgg gcacaagccg tagttcgagc cgcagcctgt ctgcgcggtg gtggattcta
     2941 tttggcgtcg ggaaagcgat cgcagtaggt cgtcacttcg ggatacaaat cggtgccgcg
     3001 caacacccgg cagccaaaac cgagcagtcc atcgacgctg gcgaggagga ttcgcgccgt
     3061 accatcgcca ccagccaaaa tgacgcgggt tccgtcgggc gtaaactgga cactgcggac
     3121 tccaccggct ttgtcttcga gcgtgagcag cagcttgccg gtcttcactt cccagattcg
     3181 cgccatgtga tcgtcgccgc tggatgccag caggctgctg tctggggaaa aagcgaccga
     3241 gcggacgacg gtgttgtgtc cttgcaggtt ccactgcgcc tgtccggttc gggcatccca
     3301 taccatcacg ttgccgtcgg cagatgcggt cgcaatgcga gccccgccgg gcgaatacgt
     3361 cgctgcgagc acatcttggt tgtggccttg cagcgtcagc agcgtctgtc ccgtcgtcaa
     3421 atcccagacc cgggcgttgc cgtcactcga cgcagtgacc agccggctgc cgtcgggcga
     3481 aaacgaacaa gatcggactt cgctggtgtg gcccgacagc gtcgatacag gctgaagagt
     3541 ctgggtgttc catagctttg ccgtgcgatc ggcgctggcc gtgcaaagcc gtgttccgtc
     3601 gggggcatag gcgacttgga agaccgggcc ttggtgtccg ctgatggttc cgacggggcg
     3661 tccggtcatc ccgtcccaca gcttgacggt cttgtcgctc gacgcggtgg cgatgcgctg
     3721 gccgtttggg gaaaaggcac cccagttgac ctcggcggtg tggccggcga gcagcgcttg
     3781 gctgcgctcg cttttggcat tccacaaaag tgcgttgtga tcgatgtggg tggtgacgac
     3841 gcggccacca tcgggcgaaa acatcgcgct gttgagctcg aacttgcttt ggaaaatctg
     3901 gccgtaacgt ccactgcgga catcccagac gcggacattg ccgtcggatg atgcggtggc
     3961 gacgcgcgcc atgtcaggcg aaaacaccgc gccgcgcaag tcctgggtgt ggcccttgag
     4021 cacgtacagg gcgcggcctg aaatggcgtc ccagacgcgg gcaattcggt ttgttgtggt
     4081 ggtgacgacg cgcagtccat cggcagaata cgcggcggtc cgcactccgt cttcggcatc
     4141 gaagatgttg agatgcgttc ttcccgtcgc ggcgtcccaa atgatcgctt ttttgtcgcg
     4201 tcccgcggtg agcactcggc tgccgtttgg ggaaaaaatc gccgacgtga cgtcttcgct
     4261 gtggccggtc aggatgaaaa tgcagcggcc cgaaaaggcg tcccaaatgc gggcggtgtc
     4321 gtcggcgctg ccggtcacga cgcggctgcc atcgggggaa aaacctgcat acagcacagg
     4381 tccgtcgtgg ccgctgagcg tgaccatgct gcgaccggtg cgcgcgtccc aaattcgcgc
     4441 ttggttgtcg ctgccggcgg tgacgacgcg gttgccatcg cgagagaacg ccgcgacgtt
     4501 gatttcttgg ttgtggcccg tcagcgtgac gagcgatttg ccggttgcga cgtcccaaat
     4561 gcgcgcggtg cgatcggagc tggcggtgac gactcgcttg gcgtccgtcg aaaacgccgc
     4621 gtgcagcact tcttggctgt gccctttgag ggtctgcaca agctgcatgt tggcggtgtc
     4681 gtagacgcgc gcgctgccat cggaaaccgc agtgaccagg cgttttccgt cgacgctgta
     4741 atgtaccgaa tagacggacc cttcgtgttt gaggatctgc gaaacgacca tctccccggt
     4801 ggcgcgaaat agctctttga ccgaagcggg ggttggcgaa gcgccgccag ccagcatgtt
     4861 tccgacggtg atgatcgcgt tttttagcac cgtctgacgg ttgccgattt gatccaaaaa
     4921 cagatcattt tgcgcttcga gcgcttcaag cttggctttg acgtcgggat cgataggcgg
     4981 cggcggtggg gtcaaatcga cgctggcatc ctgtccgcca tcgagtacgg acagatccgc
     5041 gtgtgtgccg ccatccgggc cattggccgt tgaagtggcc gcgctgtccg tcgcgcttgg
     5101 gctgcgcgtg ccgccgtctt tgttggccgc cccagcagtt gcggcgttgc tcgcggcgga
     5161 cggattcgct gccgtcggcg ttttttcctc ggcagccagt cccgcatgaa ggatgccgag
     5221 caggcccgct ccgccgagga gagtctgcaa aaaaagccgt cggaaccgca aagacatgac
     5281 cttcaggata tcagagcttt gttttcccgt tcccattccg atgacaaatc ccctatggtt
     5341 ctgcccgcaa aaccgaccct tggccccgtt ctgctggagt ctaagatgtc tgctttctcg
     5401 tcgcgcttgc ggattgtttc gctgctcttt gtgccgcttc tggctgggcc ctcgtgcacg
     5461 ccgtatgagc gccgcagtgg ttcctacagt gctggcgcgg tcgacccgat acactttccc
     5521 gccccgtatc ttggtgaagg tggcgatggc aaaagaccgg gcagcgggcg ctttcagtac
     5581 gtgacggctt atgcgcggaa gatgcccgtt tcgtactatc cgctgcgact gtccggcgag
     5641 caggctgcgg ccagcgatcc gctcgatgta accaatgtgc cgctgccgct cggctatcag
     5701 tttgatcccg atcgatcgcc cgatggccaa gacagccgtc gctgcgtgtc gccaccgggt
     5761 tatgtctacg atcaggaagc gcgccgccaa aatgccgtgc cactcgatcg ccaaggcaac
     5821 atttttaccg cgctgcccgt cgaaagcgat ccgcccggca agacgacgta tgtgccggtg
     5881 gttcgcgaag tcgtcgtgca gtcggacaaa aatccctgcc aagaccccaa gagcgaagac
     5941 aacctgaccc atcgcaccga tctggcgatc gatttggtgc cgccgccggc gggcgcagtg
     6001 ggggcgattc cgacggggaa accgagtggc cgttatcttt tgtggatcgt catcgacccc
     6061 gcggcagagg tgctatttcc cggcggcaag ctcaattcgg tgacgcagct tggaccgcag
     6121 cgctttggtt ggtttaacca gtacttgacc gcttatctcg atggtggagt cgttccgctg
     6181 gatgtcctga caccgccaaa caaggtccgg ctgaagacgc agcggctttt gtatccgcag
     6241 cgcgtgctcg atgccaagtc ggggaaggtc gtgatgggcg agctgggaat gggctttgac
     6301 cttgtcgagt tccgtcgcgg tgaggaaggc tacagcccgc tgtgtcacgt ctactcgttt
     6361 gaaccaaacg atcccatgat gggttttgag cagtcgattg ccgaggtaaa tcccgcgcgg
     6421 atcgtcgata cgggcgaagt cgtctactgc ccgcagcctt cggaggcgcg ctaaaccatg
     6481 cgtcggatat tctcttccct tgcgccgctt gtgctgctgg ttccggtttc tgccttggcc
     6541 attggtgagc tggatggtcg actggccggt gtgatcaccg acaaagtatc gggggcgcca
     6601 ctgccggggg cgcgcattcg ggtatctggt tcgcgcttga ttggtgggcc gcggaccgtg
     6661 gccacagacg acgatggttc ctatcagata ggtgcgctgc cgccgggttc ctacgatgtc
     6721 gagctgtcct atgaaggcgc caagccgctg cgccgcaaga tcgttatccg ccaaggcgag
     6781 tcgtttccgc tcagcattgc atggagcatc gaagaacttt cggaatcgac caaagtcatc
     6841 gtcgaagagc gcaagctgac gcatccggat tccacccaaa ccggaaccgt actgacttcc
     6901 gatcagacgg cgcgggtggc gacttcgcgg cgttatcagg acgtcgcgca acaggtcgcg
     6961 ggtgtgtctg ggggaagcaa tcccaacatc aagggagcga tggattccca caaccggtat
     7021 ctggtcgatg gactcgacat cacggatccg gtgacgaaca cgttttccgc caacatcaac
     7081 ttcgattcga tcgaatccat cgaagtgatg actggtggtg cggaagcgca gtacaactcg
     7141 cttggcggca cgatcaactt gatcacggcg gcagggggca acgaatggaa aatcagcgct
     7201 tcgcagtttt tcaatcacca gtcattttcc gtcggtcagc agtttggaac ccaggtctat
     7261 caaggcgcgc gacccttcct gaatttggcg cgtccgccgc aggccggata ccagaccacc
     7321 gtgatggtcg ggggaccggt catcaagaac aagctgtggg tcaatctgtc gtttgaatac
     7381 ctgcgcaggg agtcttccca gccgcttgga ccgacgttgg gaatccagtc tccgtcatcc
     7441 ctatcggatc gttttctggc gcggctcaag ctgacttacg caccgacggc aaaacatcgg
     7501 atcacgctgt ctttgtccgc ggacccggcg ttttttaaca acgtcgatca ggacaacctg
     7561 cgcttaggaa ttgcggaaga ttatcagcgg caaggcggcg tgttttcgat cttgcagtgg
     7621 gactatttcc acagcgattc ggtgaacacc aacttgcaag caggtgtgca gtattcgacg
     7681 gtggatgtgg gtccgcaggg attctttggc agtgtcgata catcggcgta tcgcggcagt
     7741 ggacggtttt cttctgccaa cgattcctac gatccggatc gaccgcagca ctacaacgcc
     7801 gatgatggaa cgtactggta tcagggggga ggaatctcct atgacaagcg gattacgttt
     7861 cagtttgacc cgtcggtgtc ggtgcgcggc aagctgcttg ggaaacacga ggcgaagttt
     7921 gggattcagt cgcgtgtgac ctatcactcg tttgatgcat cgacaccggg tggacagtcg
     7981 ttcagcgatg aagggggcgg gcccggcgaa agcggtctgt gtctgcagca gatgggccag
     8041 acccaaggct gcaacctgct gacgttttcc cctgcattcc gcaaccagca gcttggtttt
     8101 tccgtcggtg cgtttgcgca agaccgctgg cagatcaccc agtttctgcg cgtaaatccc
     8161 ggcatccgcg tcgattacgg aagaaccacc aacagtcgcg gggaagaggt gtccagcctg
     8221 attggctttg gtccgcggct gggacttttg tttgatctga cccaagatgg caagacgctg
     8281 tttaccgcgt actacgggcg ttcaaatgaa gtcctttccc tgctcgcggc ggcgtatgca
     8341 gacatcacgc catcgtcgct gacccagaag tgggatgccg aagcaaagac gttccgtgac
     8401 ttccgttcat cggggggcga tggcggttac aaactcgatc cgaccggaac gcctccgcac
     8461 accgacgaag tgacgttgtc actgcgccgt gaagtgctgg aaaactcggt cgcgagcatc
     8521 gactacacgt acaaacgggt tggcaacatc tgggacagca tcgaagtgaa tcagatatgg
     8581 gacccgacgg gaacgcgggt ggtcgactat gtcgatggtg ttccgcatca ggtattccgt
     8641 tacagccgtc cggatgaaaa ctggcgcgtc tatcaaggcg tcgatttcgt gtttgaatcc
     8701 aggccgcggc aggaatggga tctgtttgtc gcgtacacac tgtcgttcct gtacggacca
     8761 ggggccgagc agtttgcgca ggtgtccgga agtcaaacgc tgagccagtt ttacaacccg
     8821 cggatgtatg gtttttacga cggatttttg cctgaagacc gtcgacacaa cctcaagctg
     8881 cgcgcttcct atacgttcaa gggactgctg tttggcgcat tcctgaactt ccaaagtggt
     8941 gcgccgcgga ccaagcgctt ctacaaccaa aatgaaggtg actacacaaa ccggcgctcg
     9001 cccagcggta cagatcctgg acagaatccc aacgatgtaa cctcgattgc cgaggttcgg
     9061 acgcctgcct tgctgacgac ggatctgcgc atcgcttacg acctgcactc gcttttgcag
     9121 aaagtgcatc tgtcggtgag cgtcgatctg tttaacctct ttaacctcga tgcgccgacc
     9181 gagatcgaag cgcgcgacgt acccagttat ggtcaggtcc gcgcccgcca acagccgctg
     9241 cgcttacagc tcggcctcca gcttcaatac tagaccccgt tcattcccaa acccgtacat
     9301 tttgcgtggg gccccgctac cctgcgagac ttttttcgtc ccgcaagcgg ttcgaaaaaa
     9361 gctctccggt cgcccccacg ctcgtgactc aagctgttgt tttcggagtg gactcagggt
     9421 agaccccgta aaaacgtcgg tcagtccgat gcttgtaggg ctttgtgcag ggcttcccat
     9481 gggagggtcg cgcagcgtcg acgcgaggga tattcccgca ctcctgacag cgccaccaag
     9541 tcgcccagat cggcgcgggc ttgatcgtca cgttcgctgt ccgcagagtc ctgtaggaat
     9601 gcaaataaca gcttgctcat gtgttcgatc gcgtggcgat tctgtccttg tatcgcatcg
     9661 gtaagcagtg aagcagaggc gcgacagagc gcacaaccgt ggccttcaaa gtgacagaca
     9721 gagactacat ccccgtcgac aactgctgaa attgtcactt cgtcgccaca cagcggattg
     9781 gacagcttgg cgcgatgggt cgccgccggc agcggaccaa aatgatgcgg cgcgcgatga
     9841 tgtgcaagga tgatctcttg atacagcttg gcaagcgaag gcgtcatcgg aatagctcgg
     9901 ttgtgcggat caatgcagcc gccaagtgat cgatctcttc gacggtgttg taaaaggaaa
     9961 gcgaagcgcg gacgctgccg cggattccga gtgcggcaaa cagaggctct gcacagtgga
    10021 ggccggcacg cactgcgatt ccttccaaat cgagcgcggt tccgacatcg tggggatgaa
    10081 ttccggaaag ctgaaaggac agcacaggaa ccgtcggacc gtcgggaaga agcgccgaaa
    10141 ggccgggaat ttgtgacagc ttttgcacca tatgaagcat caaaaaggat tcatattcgg
    10201 caagctctga catgccgatt tcggccaaat aatcaatagc cgttcccagt gcgatcactt
    10261 cagcgaccgc gggcgtaccg gcttccaagc gaaacggaac tggctgaaac tctgtgtgtt
    10321 ccgccgtcac cgactgcacc attccgccgc cggtttgcca cggagaaagc gcggccatcg
    10381 cgtctttttt cccccacaaa acgccgacac cgaacggggc atacagcttg tgtgcggaaa
    10441 aacagtagaa atcacagccc agttcggaca catcaactgg cagatgcgga accgcttgcg
    10501 caccgtcgac aacagtgatg gcagacacct gtttggccag cgcgcagact gcttttacat
    10561 cgacaatcgc gccgtttacg ttggatgcat gggaacaggc caaaatccgc gtgcggggcg
    10621 aaatcagcga cgacagcgct gacatatcca gcgttcccga tgacgtgatc ggagcaacca
    10681 ccagcttggc ctgtttttgt tcacagagtc tttgccacgg gacaaagttg ctgtgatgtt
    10741 caagctgggt gacgatcact tcgtcgccag gctgtaccgt cgcaaacccg tgggtctggg
    10801 ccagcaggtt tatcgcttcg gtggttccgc gcgcaaacac cacatgggaa tcggacggtg
    10861 cggaaaggaa tcggcgaact ttgtcgcgca ccgattcaaa atcgcgcgtc gcttgttggc
    10921 tcagaaggtg gacaccgcga tggacgttgc cgcattcatc gacatacgcc gacagcagcc
    10981 gatccaacac cacctgcggt ttttgactgg tcgaagcgtt gtccaggtaa cacagcggct
    11041 tgccatgcac tgtttttttc agcgctggaa agtctgctcg gcgacgagca gcaagcagcg
    11101 cgggcggaac gcttacagac atgcgcgcac tatggcagaa accgcaacaa agcaacaccg
    11161 gaccccgatc aaagtcggtg aagttgtggc gagggaccat ggacagcggc ggtcgacccg
    11221 gcctaagctc gcgccgaaaa ggaaccccat gcctcacgag tctgcttcgc tttttgcgca
    11281 aggcctacgc tggcaatcaa gcggtcaccg ggttgcggtg gcttctgtgg tcaagacgtg
    11341 gggaagctcg ccgcgtccgc ccggcagtct gctcatcatc tgcgacagcg gagagtttgt
    11401 cggatcggtt tccggtggct gtatcgaagg cgcggtgatt gccgcggcgc agaacacgct
    11461 acaagacggg gtgccgcagc ttctgtcgtt tggggtgacg caggaaatgg catgggaagt
    11521 cggcctggcg tgtgggggcc gggtcgaggt gttcgtggaa agggccgaag ccgacacgct
    11581 gcaaacgatc gcgcaggcac tggatgcgcg aaagccgatc gtctgggtga cggcgcttcc
    11641 gagcggtcgg cgctggctct tcgcgggcgg cgacttggcg gactgcccgg ctgcggtgcg
    11701 ggatctggcg acggcggcac tcggtacgga cgaagcaaca gaggctacgc tcgacgatgg
    11761 acagcggttt tttctgttgc cgttttcgcc ggcgctgcgg cttttgattt ttggtgcagt
    11821 gcacacggcg cagcctctgt gtcagatggc gacggcgtgc ggctttgatg tgacggtgat
    11881 cgatccgcgg gcggcgtttg cgaccgaagc gcgctttccc caggtcgagc tggtctgtga
    11941 atggcccgag cctgcgcttc agcatgtggc catcgatcga cgcactgcgg tggtgacgct
    12001 gacccacgat ccaaaactcg atgatccggc gctcgtcgcg gcgctgcgca gtcaggcgtt
    12061 ttacgttggt gcgctgggca gcggcaagac ccatgcggcg cggctgcgac ggctttcgga
    12121 tctgggattt tcatccgagc aaacggcgcg gatctgtggg ccggtcggtc tgcggatttc
    12181 tgcacgatca ccggcagaaa tagcggtgtc gatcctggcg cagatcattt cggtgctgcg
    12241 gtcagatggg agtcgatcga ttccatgaag tttggtcagc ttgccgccgc agtcgggctg
    12301 atctgtgccc atacgctgcg tttgcccgac gggacggtgt tcaaaaaagg ccgccggctg
    12361 agcccgccgg atgtcgatct gctcttgcag tgcggaatga gcagcctgtg ggcggcccaa
    12421 ctggaagacg gcgatctcga cgaagatcag gcagcggcgg cggttgcggc ggcggtcgcc
    12481 gggcccaata cccaggtggc ggaagcgacg acggggcgct gcaatgtgca tgcggcgtgt
    12541 gacggtcttt tgctggtggc ggcgccggct gtgggtcggc tcaatcatct ggacccagag
    12601 ctgacgctgg cgacgctgcc gcacttgtcc ccggtgcgcc aaggcgagat ggtggcgacc
    12661 gtcaaggtga ttccgtttgc ggtgtgcggc gcgctggttt cggcggcgca gaagctcgcg
    12721 gcgcagggtc aaaccgtcga cggcgatatg atccggattg cgccctttgt gccgcggcgg
    12781 atgggtctgg ttttgacggt gacgccggga ctgcccgaca gcatttccga tcgcgcggcg
    12841 gcggcgcagc gggttcgcgc cgagcggctc gcgagccaca tcgtcgagga gcggcgctgt
    12901 tttcatgatg aagtggcggt gtcgacagcg ctgcgcgagc ttgtgcaggc cggctgtgat
    12961 ccgatcttgg ttttgggggc atcggcgatt gtcgatcgac gtgatgtgat tccgtcggca
    13021 attgagtccg cgggcggttc cgtgttgcag cttgggatgc cggtcgatcc gggcaacttg
    13081 ctgctgcttg ggaaactgaa ttcagcgacg gtgattgggg ttccgggctg tgcgcgttcg
    13141 ctgcggcgat cgggctttga ctttgtgctg gagcggacgt gtgccggcct gcccgtgacg
    13201 gcggacgcgc tgcaagacct gggcgtcggc ggacttttgc acgaagtacc gtggcgaccg
    13261 atgccgcgtg agaaagcgct tttgccgccg caggtggccg ccgtggtgct ggctgcgggg
    13321 cgttcgcagc gcatgggaaa agacaacaag ctgctcgcac tgctcgacgg aaaaccgctg
    13381 gtctgtcatg tcgtcgatgc gctggccaag accgcggtgt caccgattgt ggtggtgacg
    13441 ggacaccagt ccgatgcggt ccgggccgcg ctgtctggac gtgatgttcg gtttgcccac
    13501 aatcccgact ttgccgatgg gcttagcacc agcttgcggg tgggtctgca tcagctcgac
    13561 agcaccatcg acggggcgct gatctgtctt gccgacatgc cgcgggtgcg cagggatcac
    13621 ctggaagcgc tggtcggtgc atttgaaccc gaagacggtc gcgaagtggt ggtgccgacg
    13681 ttcgccggtc gtcgtggcaa tccagtgctg tggtcggcgc gtcactttgc ggccatgcgc
    13741 gagctgcgtg gcgatcaagg cgcgcgcgag ctgctccggc ggctggcggc ggtgacgtgt
    13801 atggtgccga tgccggacga cggtgtgctg ctcgatgtcg ataccgagga gtcgcttgcc
    13861 cacataaggc gcgtcccgct tgacaagtcg tcaggagact aaccgccgct cagccgcact
    13921 ccgacgacac cgaggatgat cagcaagatc gacgcgagct ttggaagcga gagcgtttca
    13981 tggaaaaaaa acagcccgag catcacgacg agcagcgtgc ccatgccggc ccagatcgca
    14041 taggcgacat tcatcggaat ctgcttgagc gcgagcgtca gcatgcccag gctcgccatg
    14101 tagcagagca gcatcaccac cgacggaacg atccgcgaaa aaccgttcga cagtttcatg
    14161 catgtggtgc cgcagacttc gaaaaggatg ccaataccga gaaagagcca gctcatgccg
    14221 aaaggttcta ctgcaagttg tggcgaaaaa cgtcggtcgg aaatttgtgc attttgccac
    14281 ccacgttaag gtggccgtct gaagagtcgc caatgcttct cccgacaccc atcatcccct
    14341 cctcggccat ctccttgaca ccgcttggaa cagacgggca ggatcagaaa gggcggtttg
    14401 ttgcgtaatg gcggtcagta acgcattcct tacttcgcac gcagttgcag agcttatcgg
    14461 ggtcagtcca tcgacggtgc tgtcctggat cgacaaaggt cttttgccgg cgtttcgtac
    14521 ccccggcgga caccgtcgcg tcgagcccag cgtcctggtt gagtttctgc gcaatcatca
    14581 aatgccggtc ccgcggacgc ttttgccaca ggccaggcgc atgcttctca tcgacgatga
    14641 tgcgacgttt ttgcgctcga ccaagcggca gctaaaacag aactttcccg atctggaagt
    14701 cgagatcgcc gaaggtcctg tcgagggact gctcaagatc ggtaccgatc gccccgatgc
    14761 ggtgctactc gacgcgatga tgccgggaat ggacggagtc gaagtgtgtc gacaaatccg
    14821 tcaaaacccg gccaccgcgg acatttcagt catcgcactg accggtcgcc tcgacgattt
    14881 gctcgaggct tcgttcaaaa agtcgggggc gactgcctgc ctccggaagc cgctcgatat
    14941 caatcaactg acgcagcttt tggggcttta acacatgacc gtgctcgctg tggcgcaaga
    15001 ggagagggct tcacgctcga gccagaagtt acgtgcgctg cggcatcagg ccagcgaatt
    15061 tggcgttggc ccgatcgctt gcaccgcgtg gcatcccagc ttccaggttc tcagtggtct
    15121 cgtcgaaaac ctgtcgatgg gcggacttct ggtgcgcttt cctggtgtgc tcaagctggg
    15181 gacgatggat gtcgatgact ggctgctttc ggtccacgtc tcgctgcgca cgggcgaggt
    15241 gatctgtcac agcgatgcgg tggtgcggca tgtccagacg gacggcgacg acctgctgat
    15301 cggcctgcaa atcaaaaaag gcgtcgtcga tctaccgacg ctgtatgaac ggcaaacccg
    15361 ctatcgtttt gcggcgcgct gcgatcaagc cgaacgagtg gccgacgcga gcaaaatcct
    15421 gccgcagttt aaggaatggg tttccgatct gcgcaactac ctggaacgga tgaaagcgtt
    15481 tctcgaccag gaagagtccg cgctcgaaag tgaagacctg tttacccgcg atcagatcgt
    15541 cgcgcagtat ctgggcgaag tcgggccgcg gatcatccag cgggtgcaga cggcaagcca
    15601 tcaaatggcc ggcatgctcg ggaacttgag cgacgacgaa cacgcagccc atcgtgccta
    15661 tgcgcagcat cacctgggcg agctgttttt gcatgcgccg tttgtgcgcc gtgcccacaa
    15721 aaagccgctc ggctacgcgg gcgactacga aatgatgaac atgctctacc gtccgagcca
    15781 cggggagggc tgtagtctgt ttggccgcgt gctcaacatg tgtacgacga gcgaggtggc
    15841 tgcgcgcgcc aacatcaatc gcatttcgtt tttgcaggac aaaattcgcg ccacgctgcg
    15901 cctgcactcc gcttctggca aagcgcgaat tcggattgcc tcggtgggct cgggtcctgc
    15961 caaggaaatc gaagacctgc tcgaaaatga gccgcacctg gggccgcttc tcgacgtgat
    16021 gctcattgac caggacgtgc gcgcgatttc gtattgcgaa cgtcgcttgg tgccgctcgc
    16081 ggaaaaaacc ggagcccagc ttcactttgt ccgtgagtcg gtgcgtcggc tgctctgtgg
    16141 tggtcggctc ggctacacga tgggacagcg gcagctcatt tatagcgccg gtctgtttga
    16201 ctatctgtcg gaccggagct tcgtggcact gctttccgct ctgtacgaag gggccgcaga
    16261 gggcggacag attctggttg gcaacgtcga tcaatcgaac ccgacgcgat acttcatgga
    16321 gtatttcgcc gagtggtttc tcatccatcg ctcacgccaa cagctactcg acaaagcgaa
    16381 acttctgcga cccgcgcctt cgcaggttcg cgtcgagtca gagccgctcg gcgtgaacct
    16441 ctttctcgtc cttcagaaat gacccctgac tttaagagct acctccaaga ccacaacacc
    16501 gctgcggttc ggctgacctg cattttgtgt gcagtgctga tgccggcggg cgtgtttctc
    16561 gactggttta ccaaccggca gtttgtgggg gagttcttgg ttttgcgcct tttggcgtcg
    16621 ctggtgtcgc ttttggtgct gggtctggcg cggctcgata gttttcgtcg gcacagctat
    16681 ccgcttatca ccatcgtgat cctggcctgc acgggcgcca tcgaggtgat gatcgtcagg
    16741 ctcggtgcgt cggccagctc gccgtattac gcggggctca acctctgcat tttgggatcg
    16801 ggcctggtct atgtgtggag cgcgcgacaa gcactgctcg tctgtgggtt ggtgctgctg
    16861 atgtggcttg tgccggcgct cgcgagcggt cccgaaatct acggcgcgtt tgtgaacaac
    16921 ctgtattttt tggtgctgac cagcatcatt gccgtcgctg cgaaccaaat gcgttacaac
    16981 ttggcgcacc gcgaatggga agccaagaca gcgctatcgc gaaccagcgc cgagctccag
    17041 acttcgatcg agcggattca ggaactcgac cgactgaaaa atcagttttt taccaacatc
    17101 tcgcacgaac tgcggacgcc gctggcgctt atcaccgcgc cgattgaaga gatgctgtcg
    17161 cgtccgggtc tgcccgacat ccatcgcaat gcactgcaag tggtgttgcg caacggtctg
    17221 cggctgcatc ggctgatcga cggtctgctc gatctggcgc ggctggaagc ggggcagctg
    17281 cggctgcgcg tcgccaacct ggatttgccc catcttttgc gcaatgtcgt cggggcattc
    17341 caacccgcgg cagagagcat gggaattggc ctttcgtttg cgctgcccga cgaaccgctg
    17401 ccgacatcgc ttttgggcga cagcgaaaag ctggaagtcg tgatgacgaa cctgctcggc
    17461 aacgcgctca agttcaccga gcgcggcggt tcgattgcgg tgaacgtgcg cgcagtgccc
    17521 gacgcggtcg aaatcgacat caaagacagc ggcattggca ttcccgaagg cgagcagtcc
    17581 tacatctttg agcggttccg tcgcgtcgaa tcggcaggtc gcaaacaggg tggggcggga
    17641 atcggtctgg cgctggtcaa agaactggtc gaactacacg gcggttccgt cgcggtaaag
    17701 agtgcgtcgg gcgaaggttc gacgtttacg gtgcggctgc ggctgggcag tgaacacctg
    17761 caagacgatc tggtcgaaac gccgtcgtca ccgccttcgc gtccaccaag cgagctgtcg
    17821 cgcgccgcaa gtccgcacca agcgctcgtg cacatcgatg gggcgctcga tccgcggctc
    17881 gaaccgggga tcaaaggcaa ctcgagccag tcgattccgg tgctggtttc gggcggcggt
    17941 cacagtcgag cccacatcct tatcgccgag gacaacgtcg atcttcgcaa gttcctgcaa
    18001 gagctgctgg cgccttatta caacgtcacg gcggcgtgtg acggcaaaga agcgctcgaa
    18061 aggctgcatg aacggctgcc cgatctggtg ctggccgacg tgatgatgcc gcagatgtcg
    18121 ggcatcgagc tgtgtcagca agtgaagcgc gatctgcgaa cgcgaacgct gccggtgatt
    18181 ttgctgacgg cgcgcagcgg catcgacgaa acactggaag gctttagcca cggtgccgat
    18241 gactatctgg tgaagccatt tagtccgcgc gaacttctga tgcgtgtcgc ggtgcagctc
    18301 aagctgcgac ggctgacggc gcaggtggcc aatgcggcgc ggcttgcggn nnnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc agctcaagct gcgacggctg acggcgcagg
    18481 tggccaatgc ggcgcggctt gcggcggtgg ggacgctggc ggcgggtgtt gcgcacgaaa
    18541 tcaaaaatcc gctcaacgcg atcaacacgg gggcggcggc gctgcgcaaa cgggatggcc
    18601 tagacccgtc gctgcaagcc gaaattctcg acatgatcga ggaatgcgta ggccgaattt
    18661 ccgaaatcac gacggcactg accgagcacg cgcgccctgc cgatggcgaa ggtctgtcgc
    18721 tgttcgatgt gcaaaaagga atcgaaacga cgctgcggct cttggctgat cgcttgcgtg
    18781 cgtcgtcgat tcagatcgtg cgcgactacc ggaccgaacg gctgatcgtg gcgcgaccgc
    18841 ggcagctgaa tcaggtgttt ttgaacctgc tcgacaatgc ggtgcgggca agtccggctt
    18901 ctggacggat ttcgctgacg ctgtcggaaa cggatcgcga gacggtttcg ctggtggtcc
    18961 acgacgaagg ccccggcgtt ccggtcgaaa accgcgagcg catctttgat ccatttttta
    19021 ccacccgagc ccccggcgaa ggaaccggac tcgggctcta tctgtctcgg cagatcatcg
    19081 aatcacacgg cggctcgctg cgagtcggca gtgtgccggg ccgaggcgct gcgttcatcg
    19141 tcgagcttcc ggtcgcagga ccaaccgcag cgcttggcgg aaccagtagg agcagtgagg
    19201 gagcggcagc atgaaaccaa gcaaaccagc accaagcgga gagcgccgcg ccgtcgttct
    19261 gtatgtcgac gacgaccgcg ccaacttgct ggcgtttcgt gcgatcgcgg aaccgaccta
    19321 cgaagtggtg gtggccaaga gcggcgacga agcactccag cttatcgagc aaaatccgga
    19381 catcgcggtg ctgatggccg accagcgcat gcccggcatg agcggcatcg acctgtgcga
    19441 gcgcgtccag ctgagccatc ccgacatcgt gcggatgctc gtcactgcgt attcggattt
    19501 gcaggcggcg attgcggcga taaaccgcgg acacgtcagc cgttatctgc acaagccgtg
    19561 gaacgccgag gagctgttgg cgacgattcg cgatgcggtc gagcgctatc ggctcaaagt
    19621 cacggtgcaa gaactgcagg tgcggatttc gcagagcgag cggctgtatg cgctcggggt
    19681 gctgacggcg agcatcggtc atgaaattcg cacgccgctg tcgattgtgt cgtcgaatgt
    19741 gcagtttgcg cgcaaagcgc tcgtcgacgc gctgaaaagc ccgcagcttc gcagcgccgg
    19801 ttcgggcctg caagaagtcg aagccgcgct catcgatgcg gaagaaggcg cccagcggct
    19861 gatcgacatc gccgatagca ttctgttgtc gtcgcgcagc gagccgggcg atcgcgaacc
    19921 cgtcgatctg tcgcatgtgc tgcattcggt gctgcggctg acccgttccg aagcgacgca
    19981 ccgggcgcgg ctgctgattg cacagtcggc caagccgcgg atttacggat cggcgacgcg
    20041 cgtcggacag gtgcttttga acttggtgct aaatgcgctg caggcgctgc ctgatcggcc
    20101 catcgaacgc aacgaagtga agctgtcgct cgacgaaaaa gacggtcgcg ccatcgtcga
    20161 agtggccgac aacggcatcg gcatcgagcc gcagaacctg gcccacatct tcgatccgtt
    20221 ttttacgacc aagaaaaagg ggacggggct gggcctggcg atttccaaac agatcgtcga
    20281 agaaatgggc ggccaaattc acgtcgagag ccgccccggc caaggcaccc gcttccgaat
    20341 cagctggccg ctcttggcgt cgacgtagcg gttaggcaaa aatcagccgg ccactttcgg
    20401 tgacttggca ggtcgtaagc cctgatcgtg gcgtgacggg cgctggctgg ctcggcagaa
    20461 gcgccagacc ttcgcgcagc cgactcgact tgctgggcag gtgcaggtgc agcgcggctt
    20521 ggaagcggtc gagaaagtac tcgatcgcca ggtgcagcac ccgtcgacga tactgcgact
    20581 cactggcggt ggcttggctg cgaatgtcgg cttccagtgc gcgcagcacc cgctcttgcg
    20641 cggccaaacc caagagctgg gccggctgaa gtcccgcggc agcaatcaca tcgggatgtt
    20701 gtgacgcggc gagcaggaat cgggcaattc cttcggcgac cttttggcca tcggtcagcg
    20761 tcagctcgtt gcccagaccg aactcggctt tgattcccgg ctggtttttg ccccgcagtc
    20821 cccgtcgcac cgcatcacga tacgcttcca gcagcagcgc gccggtccgc accgtatcgc
    20881 cgtgacgggc cgagcgcggc ggctcaatcg acagcggctc acgcagcttg ggcgcaaggg
    20941 gcgtcagtcc ttgcagctcc tcggcgatgc gcagcacggc accgcacatc tcttgggaca
    21001 gtgcgacttc accgtgcagc gtcggctcag cggcaacgaa cttgagaatc ggtgccagcg
    21061 cggtcagaca gcgatcgacc ggcagcttgg gcccgattcc ttcggcgtgc agatccttgg
    21121 cgcgtcccag caaggtcgca agtgccgccg aggccgaagt cggtcgtcgt gacgccaccg
    21181 cccggatcaa catcgctgga tgggtggacc gattgggttg ctccgttttg gcacgctgtg
    21241 ggcttggcat gtcggttcct tgaggtagca agatgtagga caaaaaccta cggcaccttc
    21301 catggcttgc caaatttttg tgttacgctg cgcggcgaat gatggaagcc aaggtcgcgc
    21361 tggctgcttt tgtgccgctg cgcatcggag ttgggcagtt tgtgctcgcg caagcgatgc
    21421 agcagattgc ggcggggtgg ctggtgcagc cgctgctgac ccgccatctg gaatcggtgc
    21481 aaaagggaga gcggggcctg tcggcgcttt cttccgtcgc cgcgcatgtg cttgcgcatg
    21541 tccagcttta cagcgtggcg acgtgtacgg cgcagattgt cttcgggctg ctgcttcttt
    21601 tggggctgtt tggccggacg gcggcgggac ttttgctttt cgggcatctt ttcgcagcgg
    21661 tgcttttggg gccgcggctt ttcgacagtg cgacgatgct gctgtgtctt gggcttgtgt
    21721 cgctgtcgct tgtgccatcg gggcgagtgc ttggactcga ctccgttttg gccagtcgtc
    21781 tgccgagctg gctgcgctag ccgctacttg tcgacttttt gggtgatgac gtaagcgggc
    21841 agctcgacgg atcgattgtc gatgaagatg ccgcgattga gcatcagcgc gacctgcggc
    21901 gtcttttccg acactttgta ggaaagctgg acttcgtggc tggcgcgggg cggcagcgtg
    21961 taagtcggcg cggtcggaat gccaaacacc atcgcattcg atcgggccag catctggcca
    22021 cttggcagcg cgcagaggat ttggttgcgg tcgatgacca taaagcgatc ggtctggttt
    22081 gtcataagca gcgaaatctc cagatcgtca tcgtcgtgct cgatcttgat ctggctgatg
    22141 ctgacgccgg aaaattcgta acggggcggg gtgacgtact caatgtgggc acatccaaca
    22201 gccaggtaga gcagcacggg ggcgaccagg cgcggcaaga tccggtgtag acgcatcgag
    22261 ggaactcctt ttggcgctca tcgtaacagc ttcggtcgga agctgggctg gcagcgtttc
    22321 atcgtagtca gccgcggctt gggtgtcata aaatgatgca cgccgatgaa gaaactccag
    22381 cttgctgccg ccgctgtgct ctgctttgtg ccgttgcttg tgggctgcgg aaaaaagggc
    22441 gtcgacgccg aaatccaagc gttcaaagac gccgggcgca ccgtgtccga atttatcgat
    22501 gccgacgctg gactgatgca ggcgaagcgc tgccgacagg gttcgatcga ccaaatggcg
    22561 gtgcttctct gcgaatatcc aagccgtgac gccgcgtcgg tcggggttcg caatgctgca
    22621 acgtgggcgg gggactgtcc gacgtggctg tcgctgcagc gtgacaaggt cgtgttcacc
    22681 gttgccgatc gcagcgaagt cgatcaaaac ggcaagacca tttcggcgct gtccaaagta
    22741 ttccgccggc ttgcgaagaa gtagtgcatc gccaaaaatc gccgctacgt tagcgccatg
    22801 tcttccgctt cgcctgcttc tgggctggcg ccacgccgtt tgggtcagct catccgaggt
    22861 gctttcgtgc gctttatccg cgccgaggcc gcttcgggaa tcattctgat cgtgaacacg
    22921 gtgctcgcgc tgtggctggc caacagcgca ctgcatgggc gcgtcgaaca aatctggcag
    22981 cttgcgtttt cgatccctgg aatcggcgcg ccgctgtcgc ttcgtgagct ggtcagcgat
    23041 ggactgatgg cgctgttttt tctggtcgtc gggctggaga tcaagcgcga gctgatagac
    23101 ggagagctgg cctcggtccg gcgcgcggct ttgccggtgc tgggcgcgct cggcggaatg
    23161 cttgcgccgg cgctgatcta tttggctttt acgggtggca gcccgtttgc gcgcggtttt
    23221 ggcattccga ctgcgacaga cattgcattt accgtcgggg tcatgacgct gctcgggcgg
    23281 tgcgttccac ttggcgccaa agtatttgtg accgcgctgg cgattgccga tgacttgggc
    23341 gccgtggtgg tgattgcgat tttctattcc cattttggga gtcttctgtc gatgcttccc
    23401 gtcatcggac tgacactcct tttgatgggt cttcacctgc tgtcggtccg tcgcttggtc
    23461 gtgtatttgc cgatcggtgc gctcttgtgg cttgcgatgc atcgagcggg ggttcacgcg
    23521 acgctttctg gcgtggtgct ggcgatggcg attccgtatc gcgaggggca gggcacgtcc
    23581 gcttcctctg tttcgccact gcatcgcttg gaacagacac ttcatccggt ggtggcgttc
    23641 ggaatcctgc cgctgtttgc gctggccaat gcgggggttt tgctgcctcc tcctgcgctg
    23701 gcgtggcagg tgtttcattc ccagccggtg acgctcggcg tgttttttgg cctgcttttg
    23761 ggaaagccga tcggaatttt tgcgtgcagt gtgctggcga tccgtttggg aatcggcacg
    23821 ctgccatcgc ataccaccta tcgcgtgctg tttggttccg cgatgctggc aggaatcggt
    23881 tttacgatgt cgattttcat cacggggctt gctttttcgg atgcatcggt cgtcgctgcg
    23941 gcgaaaaccg gagtcctggc tggatctgtc tgttcggcac tgctcgggag tctgtttctg
    24001 cgctttgttt cgcgcgcaaa tccagcggag tcagtgccgc gcgatgaaac agtttcagac
    24061 agtccgtcgg atcgttccgc ttagcttttc ttcgatgtga tggggtccgc cggggggcgc
    24121 agcgatcctt tgggcagcca ctttgtcagc agctttgtca gctgatcggc atggatcggc
    24181 ttgctcaaaa aatcatccat tccggcttcc gtgcagcgct ggcgatcctc atcgaacgcc
    24241 gcggctgtga gcgcaatgac cggcagtcgt ggccgattct gctctcgttc ccatgcacgc
    24301 agtcgccggg ttgcttcgac tccgtccatc agcggcattt gataatccat cagcaccagc
    24361 tgcggcggcg cacggtccgg ttggcgggtc agtacttcca gtgcttcgac tccgttttcg
    24421 acgctctgga ccgacacgcc ttgcctcttt aggaatcctt cgaccaccgt tcgattgatc
    24481 atgttgtctt cgaccaccag cacgagcgct gcggtcttcc catccgcgcg tgcattctgg
    24541 gtcggtaaca ttgcattccc aacgggctcg cgcacgggct ctgcttcggc tgcggaaagt
    24601 acctctacag ggacacggaa aaagaaccgg gaacctcttc cttcttcgct ggaaaaaccg
    24661 acttccccgc ccataagatg cgccagattg cggacgatcg acagacccaa tccggtgccg
    24721 ctctgctgtc gcgtgttcgt gctgtcgagc tgcgaaaatg gctgaaatag cagcgcttgt
    24781 ttcgtcggtg cgatgccaat tccggtgtct gttacagaaa actcgatgcg cgctgtgtgg
    24841 gcgtcatttt ggatttcgat acccgtgact tggacttcgc cgcgttcggt gaacttgatc
    24901 gcattgctga ccaagttcac cagcatctgt cgcagtcgca ccgcatcacc acgatacgtg
    24961 cagtgtggac cattccatgt ggccttgagt cttacgcctt tgcttgcggc ctggatttca
    25021 aaaagctgga acgtatcttg aatcaccgtc gccggagaaa acttggttgg gatgagcgcc
    25081 agcttgccgg cttcgacttt ggacagatcg agcacgtcgt tcagcagtgt caaaagcgtc
    25141 tgccctgacg acagaatcgc gcgaacatac tgctgtctct gctcttctgg gattcccgat
    25201 gacagcaaga gctgtgacat tcccatgatt ccgttcatcg gtgtgcgcag ttcgtgggac
    25261 atcgtcgcca ggaatgagga cttggctcgg ttcgctgctt cggcctggcg ctgcgcttcc
    25321 tgtagtttgg cgaccaattc cggcagcggt acaaggacaa tcagaatcga caccagacca
    25381 tgcaaccagc cgatgggacc agcaccgaga aaatagactt tgggggccat tcctttggcg
    25441 gaaaggacaa gataaaggag ccatgccaaa aggtaactgc ccgcgaaaat cgctccgtct
    25501 cgtactcctg cgacaaccgc gagtacaaca gcaaccgcca tcacaaacac catgatggtc
    25561 ggctgcatcc caagatacaa cgtgacgacg gcggcgatcc acgacagacc ggcgaaccag
    25621 cgcagcgctt ttttgggggc tccgcgtcgg ctgatccgcc atgtgaacat catgaaacca
    25681 agcacgatga cggtcgtcaa accgctggcc agtttgcgca caaacagaaa cggaacgccg
    25741 ataaagagca gtatcaggaa cgaaactgca aagacactga agaaccgatc gagcagacga
    25801 atgctcgtct ctgtgtcgat cgccgattcc gtgaagtagg acttcataca gagcccagcc
    25861 tgcgcgcaaa acgcaaggcc ctcggctcca ttttgcagcg acggttttcg gtctgcaagg
    25921 cgactgcatt cgcaccagaa ccgatagagc ccccgcttgg gtcaggcaca agcgtcaggc
    25981 gacgcggcag gatgtgatga agggaaaaga agttgcgatt tggctaggcg ttcaggacca
    26041 ggccagagat cgcgtggcga ctgattcgat cgagcgcgag cccaagctct tccaggtcgg
    26101 ttcgggcggc tcggacgacg agaagtgcgg ttccaccatg acggagcaaa aggcgcgcat
    26161 ctgccgcaag gctgagtggg ggcgtgtcga caatcacgta gtcaaaggca gcctgcaaat
    26221 ctttgagcag tgccgcaaaa gcaggtgagt tttcgacggc agcacggttc tgccgctgcc
    26281 ccaaacctgt ggccggaacc aagcacagat caggggtgac ttgccagacc tcgggcttgc
    26341 gttcgcggat gtccgatggc gtgggttcac tttggggcat catcagcttg tgcagacgcg
    26401 gcccgagcag gtgcgcatca atcacggcga ctttggcacc gcccccttcc gacattgcca
    26461 gcgcaaggtt tgccgccacc gttgactttc cttcgctcgg ttggctgctg accaccatca
    26521 gcaggcgggg atttccacgt tcctgaagtc gacagcacag cagtcgatac tgctcggccc
    26581 gtgcgccatc gggattgctt aagatcacca gatccgcaga cggctgggtc gatggggcag
    26641 gatggactac gtaacccaga tcgacaagtc gcgatgcccc cgtttttctg tccgctgctg
    26701 gttccggatt cccaactgct gcgtgtcgct cccccgattc gccgctggtg agcacttcgg
    26761 gcagtacgtt gcgacgattc cttttgtcgc tggacggcac ccgcgcgagc accggaagcc
    26821 gcgccgcatg ccacaggtct atcgccgaat aaatccgctt gtcagcaagc gaccgtgcga
    26881 cgccactgag cagtcccacc acaagtccca gcagcgccaa aagcgcggtc atcatttttt
    26941 tcgacagacc gcgcggacga ttcggaaagc tcgcgggatc gatgattcgc gccgcggcgt
    27001 tgccgggcat cttgatgagc tgctcactca ccaccgattc catctcgcgc tcgcgcagct
    27061 tgtcatatgc ctgccgcgcg ctttccatgt cgcgaaacag tcgcgcgtac tgtgcttccg
    27121 cattgagcgc ttcttcatcc gcagacagct ttgcatcgcc ccctgtgagc ttgcgccgga
    27181 tggtcatgat ctgccgatcg agcgccgcca tctgtgcacg cgcttggccc agcaccatca
    27241 ggtcttccgc gagcttggga tctttgcgcg ttcgggtatc acgtatgtcg cgctggatgc
    27301 gatcgatgtc gtttgatacc cgtcgatatt ccggatagtc cgcggtgtac ccttgtcctt
    27361 ttagttccag aagctgctgc tgaagtcgtt cgatctccag cattttctgc tgcgttggat
    27421 cgtacttgtg cgcattttca atctgcgcaa tctgcgcttc aaactgcgcc cgtttttcca
    27481 gcagcggacg cagctctggg gaagaataac tttccgccat tttcgtcggt ggcgtaagta
    27541 cgcgattgcg tgtcgtgacc agcagtttgt ccgctgcttg cagtcccagt ttgctgcgat
    27601 cagtggccga aatcgacacc agcagcttgg gatgcatgcg gacaaactgc accacttgat
    27661 tttctgcatc gagcatccgc tgtcgggatc gctcgctttc atcttgcaga aacatccggg
    27721 cgcgcttggc ggtctcgatc cgctgcgcga tgactccttg cacgccttct tccgacagtg
    27781 tctttaacac caactgcgaa aggtgcggat cgcgcgcgtt atacgtcatc acaaacagcc
    27841 gccgcgaaat cgcttcgatg agcagcgttt ttttgatgtt ttcgaccagc tcgtcctggc
    27901 ctttttccgt ctgaagctcg gtgagatgcg caagctctgg ctgtttcaca agcgccgcca
    27961 cactggcttt tagctgcgca tcaatgacaa gccgcgcccg gaatgcgttg gcgagcgttt
    28021 ccggaagatc cgccaggtct gacgagtctt cgcgctgacc cggaacggcc ttcgcatcca
    28081 gttcctcacc gacgcgaact tgggcttggc tttggtatgt tggtcgatag cgctgggcgt
    28141 aataaagtcc accgctgact gccagcgccg ataccgcgag catcaccggc cactgacgca
    28201 aaaatcggta gaccatgttc caggcttcgc tgcgcagacg aagcgaagca gtgtggctct
    28261 tgctcaccat gatgcaaagg ggctcgaagg aaagaggatt ggactgccga acgacacggt
    28321 cggtaagcta tatccctcgg cggcgacgca ggtcaaggac agatggcaaa gcaaacgcag
    28381 cgcgccgatg tccggcggga cagtcgggct tcaggttgtc gtcaggatgt tttttgtgtc
    28441 gacggtcttt gcggggaagg aacgatcagc gtcgcatgca gacgacacta aagcggggtc
    28501 agcgtcgaaa tttccagcag gactttttca aatgattccg cgaaccgcga gaggctctct
    28561 gctcgatagg tgccggtctt gtgaatttct tcttccagaa ccgtggcatc ttcgtacagc
    28621 cgcatcgctc ccaagttgcc cgcgccaccg cgcaagcgat gaatccgctg acgcgcgccc
    28681 acatagtctt cggcttgcag cttgtcgcgg atctcttgca gcaggtgttc atgtccactg
    28741 tagaactgga acaggagcat cttgacgaac cccgcgtccc tgccggtcag tgcaaacagg
    28801 ttctcgaagt cgaactcggt gagacgtagg agttcgtcca taacaagcca ttccttttga
    28861 tggttctatg ggaaaagagg cattccccag gcgcggtgct gtcgcttggt tctgcgcttc
    28921 ccttacgaac catcatacct aaccgccgac tttgcgccca aaacaaattc ccgatgactg
    28981 ctgcggccca cagaaccgta cacctacgcg gcgaaacaga gtgcgctgac atgcaccgca
    29041 atgtgcagat acaagcggcc caggttccgg tctacggggt ggccaacgtg ttcaaaaaaa
    29101 tgtagcgctg gccgtcgctg ctcaaagcat attgccagct tccttgcgga cctggaagca
    29161 gcggcaaaac cgcttccgga tcggcgccac agggaatgcc caaacggatg atctctgcaa
    29221 tgatcgcggg cttcactttt tgcaaaaggg aagcggacag tcgcactttt cctcggtatc
    29281 cgggggtgcc acgcgcgtag ggacagtctt gcgccaaaaa tccgcccgaa agtgcgcttt
    29341 cggcgctgtg acaccacgga gctttggcat aacggtcttc atagaccgtg cagtggtagc
    29401 gaccgtcgga cgctttgcca agaaacttgc agtgcagttc atcgagcacc acggccagac
    29461 cgttgaccgg aacagcaaag tgacacgaca tgccacagcg tcggcacagt gcttcatgtt
    29521 cagcggtttc catcgcgaca ttatcgcacg atttgctggg cccagggttt ggcaattccg
    29581 cgcgtgcggc gaccatactt tgccgtatag tccgctgcat gacgcacgac caaattgatc
    29641 cggctgagct tacgccgcgc aagatgtatc ggctgctgat tgggcttgtt acgccgcgac
    29701 cgattgcgtg ggtcggaaca atctctccgc aagggattcc caacctggcc ccgttttcct
    29761 ttttcaatgg tgttgcggcc aatcctccga cgctggtgtt ctctgtttcg acccgtcggg
    29821 atggttcaca caaagacacc ctgcgcaaca tcatggcgat tccggaattc acggtcagta
    29881 cggtgtccta ttcccatgcc gaacagatga atgcgaccgc agccgagctt ccgtacaacg
    29941 aaagcgagtt ttcccatgcg cacatcgatc cggttccctc tgttcgggtt cgtccgccgc
    30001 gtgttgcggg ggccaaagta cagatggaat gtgtgctgca ccagcttgtt cccgtcgggg
    30061 aagggccact gtcggcaacg cttgtgatcg gtcggattgt gcttatgcac ttggaaaacg
    30121 gcgtgctcga tccacagggc gatccagatc cacaagtgct cgatcacatc ggtcggatgg
    30181 gtggcgacgg ctactgtcga atcggtgatc gtttttcgat caagcgtccc taacgacagc
    30241 cgcgtggtct ttatgtcgcg cactccgtca gtgcgcacgg gcgattcctt tggacttttg
    30301 aaaattccgt cgggtttgca atgcaagcca aactgcgcta aagcgggggc ggttctgcct
    30361 gtttgcttcg tcaaaggagc cgcgctgcgg gaaaacacat gaagaccaca aacttgttct
    30421 tttgggtcgg aattctgtca gctgggctga ctgcatcggg aagctgcaat cccaatcggg
    30481 cgattcagga atatgacacc gatgggacga tgagcgaagc ggacatggca cgcccacgac
    30541 cgtatgattt tgcgctgccg tgcgttggcc tggcctgtca aaaacccaag tgtggcggct
    30601 cgaactcgac gacgctttca ggaacggtgt ttgcgcccaa tggtcaagac ccgctctaca
    30661 acgtgctggt gttcgttccc aatggggaac tcaagccgtt tgatgatggt gtcgcttgcg
    30721 agacgtgcga aaagtcggtc agcggaacgc ccctgactgc gactttgacc gataccaagg
    30781 gtcgcttcac gctcaaggac atgccggctg gggcgaacat tccggtcgtg atccaaatcg
    30841 gtcgcttccg acggaaaatc acgattccgt ttgtcaccca gtgcaccgac aacacggtga
    30901 tgaacgggga cgcacgactg ccgcgcaaca gcagtgaagg cgacattccg cgcatcgcga
    30961 ttgtcacgtc aacctatgat ccgacagagt gcatcctcaa cgcgatagga atcgacgcca
    31021 acgagttcac ggctccgagc ggcagcggtc ggatccacat ctatcacggc agcggcaaca
    31081 acatctctgg ggccccggcg ggaaatgcac tgtggggcga tctgccgacg ctgaaaaagt
    31141 atcagctcgt ggcgctgcca tgcagctcct atccaagtgg tgccgaccct ggggtgcaga
    31201 atctgttcga ctatgccaac gcgggcggac ggctgtttgt gaccgacttg tcctatccgg
    31261 tcatttcgca aggcaaaggg gactggccat cgactggcaa ctttgcaagc cccggcagtt
    31321 tttccaatcc ggcctcgatc gacacgagct ttcccaaagg caaagcgctg tctgactggc
    31381 tggagggatt tggtgcggcg acgggtggat ccttgtcatt gtcggaaacc tttagccgcg
    31441 tcggtatgat caaaccgccg gcccagcgct ggctttattc gggcggcaac acccagtcgt
    31501 atacgttcaa cactccgacc agcgttgctg aaaaagacca gtgcgggcgc gtctactatt
    31561 ccagcttcca cattggcagc ggacgcagca cgaccggcac attcccaagc gcgtgtcaca
    31621 gcaagcccct gacgccacag gaacgggtgc tggagtttat gctgttcgat cttacgtcct
    31681 gcgtgcagag cgattcggtg ccgccggtca ttccgtaagt tccgcgcgaa cgcctgcgga
    31741 tttctgaaca attcggtgac aagtttccta gacatgatgt aaaggaaact tgtcatggac
    31801 agcgcgccca caaaccgtct tcgcagccat cgggaacaag ctggcctgtc gcagcaggag
    31861 cttgcgctgg ccgtcggaat gtctcggcag agcgtggtgt ctgttgagtc cggacggcag
    31921 agccccagcg tcgatgtcgc gctgcggatg gccgatgtct tgggctgcgg ggtcgaagag
    31981 ctgtttggtc ccgcgcagcc gtccggtcgc ctgcttgccg agcccgcgtt tggcagcccg
    32041 gacggtcggg ttgcgctgtc acagataggg gggcgctggg tttcgtatcc tctttgcggc
    32101 agtgacatcg ggcgggctgc cgacggaatt gcactgaaga cgcggggcaa gaaaacggaa
    32161 atcgagcccc tgcgtccatc gcgtctgctg caagacaaca ccgtgctgct cggctgtgcg
    32221 ccggcgctgg ggctgcttgc ggatcggctc aactctcgcg ctggggccgg acgctttttg
    32281 tggttttccc gatcgagtac ggaagcactg acggcgcttg gctgcggaca ttcccatctg
    32341 gcaggggtgc atctgaccga cgaaaaaacc ggcgaagcca acctgccgga cgtgcggcgg
    32401 catcttctgc gcaagcccat tgaacttgtc acgctggcgc gctgggaagt ggggatcttg
    32461 acggcggcag ggaatcccaa acggattcgt tcgctcgcgg aagtgagccg gcatcgcttg
    32521 gtcagtcggg agcgcggatc gggggcgcgg cggctcttgg agcgtgagct gacaagacat
    32581 ggtgtttcga tcaagctggc gcagcaggca cccatgatgg cgcacggaca cttggccgtg
    32641 gcgacggcga tttctatggg ggccgcagat gttggcatcg ccagtcgtga tgcagcacag
    32701 gcctatggtc tgcattttca gccgctgacc gaggagcgct acgacttggt tttgccgcgg
    32761 tcactgcttg cagatctgcg catggtgcgg atgctagaac tgctgcactc tgcatcgttt
    32821 cagcgcgagc tgaccgcgct tggatacgac gcgcgctcat gcggtcagcg gattgtggag
    32881 cttgtcacat gacaaaacat tgcgcttgct gggggtctat cgcactgctt ttgggggtac
    32941 ttctgtttgc acgcaacgca gatgcccagc ccgcgtgcga cgactcggtg aaccttccca
    33001 atccggtcta tctgaccgtc ggtgataccc aagccaactt gatgcgtgaa ctgggcgcaa
    33061 agctgcgcgc gaccgaacag atcacgctga tctggagagc caccggatca tgcaccaact
    33121 tggacacgct ctacggcgat gtgaagatga gcggaaactt ttcttacatc cccgcggggt
    33181 acgatccaca gaccactccc aatcctccgg tgtgcacggt ggcggcccct ggcgtgcttg
    33241 cggacatcgc caactcgatc gtgtttttgg atacctgtcc aacccccaag ccgaccgatg
    33301 tgatggatgc gctggggccg gttcagtcgt ttgtatttgc cgttccacgc gcaagttctc
    33361 agacggccat cacggcagaa gaagcctatt ttgtctttgg ctttggcagc agcggtcagg
    33421 tcgcgccgtg gcttgacaac agcctgcttt ttgcgcgccc ggtgacaaaa ggaacggtca
    33481 tcagcatcgg cgcgtcgatc ggcgtgccag ctggaaaatg gcaaggtcag aagatcaact
    33541 tgtcgcaaga cttggccagt cagctcgcag ccgcacccag tccagatcgg gcgataggaa
    33601 tcctgggaag cgaagtctat gatggtctgc gatcgacgct gaaagcgctg gcattccgtg
    33661 cgtttttcca gcgccgcgcc tactttaccg attcgaccac ggcgtcgttt gacaagcaga
    33721 atgtgcgcga cggtcactat cacatgtggt cgtatacgca ttggctgctg aagttagatg
    33781 ggatgggaaa gccgcgcaaa gcgctggcgc agagagtcgt cgatgtcctg gttgggaatt
    33841 ccgtgacacc cgagccgcag tttgaaccgc tcgaagcgcc gatacgggtg ggactgattc
    33901 cgcgctgcgc gatgcgggtg cagagacaga ccgaaggggg cgatttttcg ctgtaccaac
    33961 cgtctgagcc gtgcggctgt tatttcgatt ttcgagcgac gggaacgacc aagtgttctg
    34021 cttgtacggg cagcaagatg tgcggcaccg gagtctgtcg ccacggctat tgtgaggagc
    34081 gctgatcatg caccgattgg gattcctgtg tgctctgctt ggctgtttgg tcggttgtcc
    34141 ggaagacaca agcgtgacaa atcccgcagc tgctgtggat cttgcgcaga gcggaccgcc
    34201 gtgtgtcagc agtccgcaga cccatgttga gatcctaaac gcctgtactt ccgcgcagtc
    34261 cgtcgacaaa aaaacgatgc tgccgctgct tcgcgctgat ggaacgcttc cccctttgcc
    34321 gtagtctgca gcgggtcctg tggccgctgt tgctgctgac ggtgtgggac gtgtccccgg
    34381 ctggggcaga ggaagcggat ctgaccgagc gggtacgcat cctggaagcc agactggcgg
    34441 cttttgagtc gcagactgcg gcgacccact ttccgtggca aaagagcgat tcctttgcgc
    34501 tccgcatcgg tggttttgtg caagcagatg cggtggccta ccgtcaagat tcggaagacc
    34561 agctcgatgg ggcaaccgga cagccgctca atgaaacgcg ctttgtgatt cgccgggcca
    34621 ggctgcgtgc agaagccgag gctcgctatg tcggtggcgc gctggaactg gatggcaaca
    34681 cggtgggcac gacgcaagtt cgactgctgg gggccgaggt gtctgctcag tttccttcgc
    34741 cgggcggtga cattccgtat gtccgtgcga cgctgggtct gttcaaaatt ccgtttggtc
    34801 gcgaagtcag acagtacgat ccagaaagac tgtttttgga acggagccag gtcgtgcggg
    34861 cgctgtttcc gggggaatat gatctgggaa ttcagctaca cggtgggttt cgttttttgc
    34921 gttatgcgct ggcgctgatg aacggggaac cgagcggaga agcccagttt gccgcgcgtg
    34981 atcccaatca gagcaaggat ctcgtcgggc ggatttctgt cgatgcgacg tggtggaaga
    35041 tggtgcggct ttcggcgggc atgtccggcg tgtacggtga agggtttgca ccggggcgac
    35101 tggccggaaa agatgcgctg tcgtttcgcg atgacaacga tgatggacag gtgcagccaa
    35161 gcgaaattct ggtggtgcgt ggacaggctg ccgttccgtc gcagaacttt ggccgcgaag
    35221 ccctgggctt tgatgcggaa gtgactgtgc agattcccaa actcggggcg ctgcagcttg
    35281 cgggcgaagt tgtgattgcg cgcaatttgg atcggggact cttcccttct gatccggtgg
    35341 cgagcggtcg ggacctgcgc gagcttggat attatgcgca gatggtgcag gaaatcactc
    35401 cctatgcttg cttgggggtc cggtacgatg tgtacgatcc agatgccgac cggcagcaac
    35461 aactgggggt gatgcggatt ccggtttcgc ctttgttttc gacgctgacg accacactgg
    35521 cggtgcagca tccgcgctac ggaagggttg cgatggaata ccagaatcag cgcaatccac
    35581 tggggcggac agcagccggc acgcccacca cactgggaaa agacagcgtc gtgctgcggg
    35641 cacaggtgaa gttctgatgc ggaacatgcg cgcacttgca caggcttttt tggggctgtg
    35701 gtctttgaca ggatgtgatt ctgcgtcatc gaaaagtgca gtcactgcgc ttttgcgcgt
    35761 cgaaaacgcc cagcgagtgg aaggcgtacc acctgctttg gacgatgtgt cttatggtcc
    35821 gcaagtcttg gccgtgacgc tgctgggttc gcgcatttgg ccgggtgaaa tcggcaaacc
    35881 actttcggga acactggcag agggatcgca agccgtgtct ctccacatcg aaggagacag
    35941 cgcgcactac gtactgcctg cgggagccgc ggatgtcaca gccaagtcgc ttccgacgtt
    36001 ttcggcgcag ctctcctttt cacctctttt gccagtggga atgacccggc ttgcggttca
    36061 ggccgtcgac gcgcacggac agtacggacc gcccagccat caggcgctgt gggccgaacc
    36121 aagcgacaca ccacaggggg ctttggtgat tgcacttggt tgggaacgcg ctgcggatct
    36181 cgatctgcat gtggaacagc cagatggaca agagctgtgg tcgcgccgca aaagtggttt
    36241 ttcgcagcca agaccgggtc gtccgccgtc ggcaggatcg atcggatatt tggatcagga
    36301 ctccaactcg cagtgtgtgc tcgatggtca gcagcgcgaa catgtgatct atccggtggc
    36361 tcccatgccg ggccgttatc gcattcgggt ggatacgttt tcgctgtgcg ccgaagtcac
    36421 tgcatactgg caagtggcgg ttcttttcga cggaagattg atcgccagtg cgcgtggaca
    36481 gagtgtcgca agtgacaccc gcggaagtca tggacaggga tcgggaacgc tcgcacttga
    36541 actggatctg cgctaacccg catccggttc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36661 nnnnnnnnnn ctgcggttgg tggggatttg gggcggtctg ctgggtttgg gttttttggg
    36721 gtgtttggct tgggctcttt tccgtcgctg cggctgcacc tggggatgat tccttttcta
    36781 ctgtggttcg cgccgacaag gcatcggcgg gatcgctgga agcgcgcgtt ttcaccaaag
    36841 atgggcaggc catccccgga cttttcgggg atgcggtgcg tgctgtggag gcgcaagcgg
    36901 gggtcagcag gccgcagctc ggatctggac agctgattgt gtggggcgct gcgccgtcgg
    36961 aaactcgcat tctgtgggat ggggtagagc tgcctgcgct gtatcatttt ggtgggctgc
    37021 ggacggtgct ggccaaaagt acggtggatt cccttgcgct gcagcctgcc gggtatggat
    37081 ctgcctacgg tcgcggtctg ggcggcatac tccttttgtc ttcttctgcg attccgcaag
    37141 gaattcatgg cgaggtatct gccgattttc tcgacgcgag tgcacagctt tcggctgcct
    37201 tttcatcccg gctgcggatg gctgtttcgg gtcggattgg ctatgtggat cggctgattc
    37261 ccttgctgtc cagtgcgccg cttgcggact tctatccgct gccgcgctat tgggatgcgc
    37321 aggggcgtgt tgtgatcaag ctccgctcgc tcgacgatgg acaggaacag ctgtcctttc
    37381 gggtgctcgg atcgggggat tcgcgaacac gggtgcaagc cagtccggat gccgccagcg
    37441 cgcagacaga gacgtggacg cggacatttt atcgaatcgc ggcagactat ctgcgcagcc
    37501 aaagtggaca gaagatcgaa gtcacgccgt ggtttgggat cgacgaagaa agctggcgtg
    37561 cgctgtttgg tggcgtgctt tcgcagctgt cccggcgaga cattcggtat ggtctgcgcg
    37621 cggtgatttc gcagcggcct ctgcgcagtg tgtcgtttgt ggtcggggcc gatgtcttgg
    37681 gtgatgttgc ggcgatttca cgcgaaggaa cgctgacacg acccccgcgg gaaggcgaca
    37741 tcgcggtctt tggacagagt ccggggcgcg aggtgagcag tgattcctgg tcggttcacc
    37801 aaggcagctt tgcggtcttt tcacagatca cttggcggta ttctcgtctg cagcttgtgc
    37861 cgggcctgcg cgcggttgcg tatctgtccg atgtgagccg actgctgccg cgaatcggcg
    37921 acacgccgcc gcttggtttt cgccggatcg actttgcgct ggagccgcgc cttcaggtcc
    37981 ggatgcaggc cgcgtcgcgg ctggcactgc atgccgctgc gggtctctat catcagagtg
    38041 ccgatgcagc ggatctgagc gcggtgtttg ggaatcccca gctcggtcca gcgcgggccg
    38101 tccatggggc ggtgagcgct gagctacagg tgttcgagcc gctttcgatc gagttcggtt
    38161 cttttgtgcg cgcgctgttt gatctgtggg cacggagtcc ggacctgacg ccaccgctcg
    38221 gtcgttcctt ggcagattcg gggagcggac tttcgtatgg ggggcagctt gttgtccgtc
    38281 tttcaccgct tcgcaatctg agcggatccg tttcctattc gatgggccgc gcgctgcgtc
    38341 gatctgctgc gaatgcaccg tggcgtctgg ctgattttga tcaaccgcac aacctgcaag
    38401 tggcgctgcg gtatgtgctg tttggtttcg gaatcggtgc gcgtctgcgc tatgcgtccg
    38461 gtctgccgcg gacggaagtc actggcgcat atttcgacgc gcgcgatgat cgatttgatc
    38521 cgctgttcgg accgcacaat gcgattcgtc tgccggactt tttccagatc gatgcgcaga
    38581 tcgatcgatc gtttgttctg tctgcacggc tgcgtctgtc tgtgctgctc gacgtgcaaa
    38641 atctaacgaa ctaccaaaat gcagaagagc tggcgtatcg ctatgactat cgtgagcgag
    38701 cctatcttcg cggcatgccg atcctcgcca acttgggtgt acaactgacg ttttaggggt
    38761 ccaatatgaa gcatttgata ggattcatat ggatgatctc ggcttgcgtt ccggatctgg
    38821 gaccgccaca gtcgctgctt gacgggccgc gcatgctggc gattcgcagt gaacccgccg
    38881 aggtgtttcc aggggacacg gtccagctta cgctggtcgc gatcgacgct gcgggggcgc
    38941 ttgtatctac cgatgtggat tgggcgctgt gtgcaagtcc caagccccaa gtcgaaaaca
    39001 atgtggtgag cgcagactgt ctttcggaca tggtgatccc gatttctggc acgacgcagt
    39061 cgcaagctgt gactgttcct ttggatgctt gtgcgcgctt tggtcccgag cttcccccgg
    39121 cgctgccagg gatgccggcg ggtcgtccgc gcgatccgga tgacagcggt gggtactatc
    39181 agccggtgcg ggtcactgcg ctgggaatgc aggcgattgg tcttgtccgg attcgctgtg
    39241 gtctggccgc agcgacgcca gaagtggccg tgcagttccg atcaaactat gttgccaacc
    39301 ggaatccgtg ggcgacaaag ctgtccattt ttgatggcga tcagatggtg tctgcgcagc
    39361 agctgccggc ttcccgatcg ctgcgactgc ggcttgtgat tccgtccgaa agtcgcgaaa
    39421 gctatctgcg ttttgatccg acaagtcaga cacttgtgca aacggcggag acactgcgcg
    39481 catcctggct gtccgccgca gggagcttta ccctgggtca gactttcgtc accgatggtt
    39541 ccgacccatc gctgtacgcg gaaacccagt ggacggcacc gtcgcagagc gggccagcga
    39601 tgttgtggac ggtgctgcgc gatagtcgtg gtggggtttg gactcagtcc tatcctgtgc
    39661 agatcactg
//
LOCUS       DAORGN010000019        37860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_391_length_37860_cov_6.660865, whole genome shotgun sequence.
ACCESSION   DAORGN010000019 DAORGN010000000
VERSION     DAORGN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 37860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37860
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_37860_cov_6.660865"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKL17_03865"
     CDS             complement(<1..521)
                     /locus_tag="PKL17_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNK43894.1"
                     /translation="MGYEPNQPIPYSHKLHAGELGLDCRYCHVNVERTEFATVPPTQV
                     CMNCHSNVRKDSPKLQPLRESWAYGKPVPWVRVHKTPDYAFFPHSRHVTSGVGCVECH
                     GRIDQMEVVRQVEPLSMGWCLECHRDPAPRLRPLDQITNMGWEPPGDKPLDVRRREFG
                     EKLAQELKIAPPT"
     gene            complement(805..2727)
                     /locus_tag="PKL17_03870"
     CDS             complement(805..2727)
                     /locus_tag="PKL17_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNK43895.1"
                     /translation="MGFYRLRVASLSLGLLGLVSCKGNPGPGLKLQIAESASQHACIK
                     TDKSGFPLSAVLRDGTVRLSVLQKQGTGWQFQCDIKATLPDDHPSIDLGTTDRSQYAF
                     FAELFDASGKRVYTGAVQGKSSAAADGSAGILPMFEVGSWNCPASGMVGPRAFHSATA
                     LPGGEVLIYGGVETSVVAGISDSMGVIDTVEMYDPAKATFQTVSVAGKGPIARAFHQV
                     AVLSATDTQVKLMVFGGVTAPRGRQVLTTPFNNAPLRLAPCGEAAPAGPELLTLKFDA
                     GKWTVTSEAVGQTDTAAFAGTTALPQGGLVSVAGAKFSSTPPCTMSIFVDLLKPTAGT
                     AVQKAVAWSGASGEVFGSGAFSSGFLAPSLTLLSKSSALALGGSWPASSTMTPPMTAL
                     LLTGLPEAPAAQTAMAQSVAGVATAFHTATRIGGPLDASPAFPVDVLVTGGFQLTDSA
                     TPSPGQPPSAANAVRVYTVKDATSAPNESAITPYQVATCGSSSAHYRPAAYEAASATA
                     SGRRVVITGGSATQVAGCNDCETASDTSLLCTLTQSSLYDATSRSLSRLPAMAVARFG
                     HQQTLLQDGGILVTGGLTRKMGQTTEVTSEAEIYNPRSSNTSTADLDDPVTLSLPAAD
                     QPGRVGVDAQKPCSLL"
     gene            complement(2762..3457)
                     /locus_tag="PKL17_03875"
     CDS             complement(2762..3457)
                     /locus_tag="PKL17_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNK43896.1"
                     /translation="MSTLSFSEKMFGTTILDGDRALLAKKTYALLLISVVMASVGGYS
                     CSHSEAMLSFFLRPVGWISALVLLNVVPYFALWASRQSPSLGIVALAADGFISGIALG
                     PLLAIANYLSPGTIPAALAVTGGVFVAVTGYVMFSKEKFSAPAGLLTGMFFALIIAMF
                     VNSFLQLSGLGLLITIGIGIFGVVSLVYATSDVLNNPSYDNPVQGALMLFAALFNIFV
                     TALRLLLRFSSRD"
     gene            complement(3546..5648)
                     /gene="ligA"
                     /locus_tag="PKL17_03880"
     CDS             complement(3546..5648)
                     /gene="ligA"
                     /locus_tag="PKL17_03880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNK43897.1"
                     /translation="MSAKKKGPSKPSPAATVAAVGSLFGDEGSREEYLSLVAELLEHD
                     RRYYVDNNPSIADYEYDQKKKRLDALEAAHPDWVVAHSPSRRVGHTPLSSFAKVERAV
                     AMLSLDNTYSEGDLRDFFGRVQRGLHGAGERRPPELVIEPKIDGIGIELTYKAGVFTL
                     GATRGDGLIGEDVTQNLRTVKNLPMMLKEKVDLLVRGEVYMPRDGFEKLNVERLAAGE
                     EPFKNPRNATGGTLKQLDSRICAKRPLRVLLYEVVGEVAGIATHAGLLDYLRRLGLPV
                     YPDTQIVGSLDALLGQVQVWADKRMSLPFDVDGLVLKVNDLAQRESLGFTARAPRWAI
                     AYKFPAQQATTKLLDVELGVGRTGVVTPVAVLKPVDLAGTTVSRASMHNWDQIARLGV
                     MVGDFVLVEKAGEIIPQIVAVVKQQRAGREAELVPILPPTQCPECGDTLIKRAGEVAL
                     RCPNTKPCPRQLREAITFFCNRDAMNITGLGDKLVESLVAAKLLRDLADLFSLTKAQL
                     LSLPRLGEKSADNVISAIATGKAEATLSRFVTALGIPGIGWVWAQKIAEQFPSLTKLL
                     AAAPDDVYRTLVKIHGFGDERAGAVRDYLKDPEARALLHKFAALGLSPVEPVASTGPL
                     LGKTFCITGTLSKPRGDFQKRIEAAGGKFTSSVTKKTSYLVMGAEPGEDKRAAAEKNG
                     VPILDEAGIEKLLRGESP"
     gene            complement(5745..7967)
                     /locus_tag="PKL17_03885"
     CDS             complement(5745..7967)
                     /locus_tag="PKL17_03885"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNK43898.1"
                     /translation="MRPTSQTELPPDSGARSRGKMLTDRRSLDESGSELWRYVAVLWE
                     RRWAVLLMLVLWPTAAWLWTKSQPRVYETKSSVLVEANVPQVLGSAVQDVVDPTPANY
                     YLMQDFLQTSRKVLTSDSLSRRVALRLRLLSEPGFFAGGTPPQTIEEAGEALLSYYSA
                     DVVAETRVLLMTARHTSPDWAKKIADAVADEFVADAAQSREVVNQRTSEQLADELDRL
                     RKSLHGAELALYEFKSKHDLLTVSMEDRTNQVARQIDKFSDALTEVKLRKLQRQSQLT
                     ELRKLKELDALHVPVLGGDMPQILGDLRRAYTEEQRRLAELKTRYQDTHPQVQQQTSK
                     VDQILRELAREVDVALSASQIRYSEALSDEQKVQNELQIVKQEGMRLSRLEIEYNKLK
                     RDADSFQKQYNLILNREKESGMVGRLRLSNIKVLDYARRPKIPVSPRVRVVLALSLVL
                     AGLFGVLLALGLDALDRTVKAPQDIESSLGLPLLGMLPSFAVDGNAKGRYPPDLYVAY
                     HPRSTVAEACRAIRTNLTFAGADQQLRTLLLTSSLPREGKTLCCVSLGTVLAKSGQKT
                     LIIDCDLRRPRIARAFGLSGTSGLTNVLVGELSLDEAIRSSEVENLSVLPAGPTPPNP
                     AELLSGQHFRQLLADAAGRFDRVLIDSPPAVPVTDPAILATLVDGVVLIVRHAQTPKE
                     AATRAAQHVLDVGGRIVGVVLNAIDVSGKGYRSYYGHYSEYKSDESPSERSAAKPATE
                     "
     gene            complement(8089..9777)
                     /locus_tag="PKL17_03890"
     CDS             complement(8089..9777)
                     /locus_tag="PKL17_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNK43899.1"
                     /translation="MNHIVRGRLLVNVTLAALLLGLLFVVSKEAQAAAWPILAVVVVV
                     IAVNTTLQVRTGSQVAELRLAMSRLVGGELEEPVPLGADEPLGNVAELMERLRKILHQ
                     RQQELSRQLGAQTKTSGDINGVLADLRNTIGQQMHALEETSASLHEMTASAKQIAQSV
                     ETLARGAEESSSSILEMAASNDEVAENMVNLAGAVQESATSIEEMTYSIREVAKNVEA
                     LSATAEETSSSMNEMDISIRQVENNANETARLSEEVSRAAGLGAQAITSTIDGMNKIK
                     GYSEGTVRVIARLGDKIGEIDKILRIIDDVAEQTNLLALNAAIIAAQAGEHGKGFAVV
                     ADEIKDLAERSSASTKDIGELIKSVQMESRNAIDAVERGAKAVDEGVKVSHQAEEALR
                     KILEAAERATQMVRDIARATVEQARGSKQVTDAIGSIAETVQQIATATSEQARGSEQI
                     LKAAERMKNITKHVERSSQEQTRGSKQITRSIESISEMVSHLHTAQREQTKGGDQILQ
                     AVEALRELARAQETRLGEFHRSIEVIARSAESLSALALARHRSATSIDSEAEDL"
     gene            9945..11228
                     /gene="eno"
                     /locus_tag="PKL17_03895"
     CDS             9945..11228
                     /gene="eno"
                     /locus_tag="PKL17_03895"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNK43900.1"
                     /translation="MTEITQLVAREILDSRGNPTVEVEVELSSGAQGRAAVPSGASTG
                     THEALELRDGEAGRYLGKGVRRAIENIVEVIEPAILGMDAANQGALDAALLQLDGTPN
                     KSKLGANALLAVSLASARAAAHAHELPLYRYLGGISARLLPVPLLNLINGGAHADSGL
                     DVQEFMVVPYGAPSFPEALRAGAEIFHHLKKVLKGQGMATSVGDEGGFAPALPRNEAA
                     LEALMTAIGKAGYKAGVDVGIAMDVAASEFFSADKGIYKVDGKELSSSELVAWYVDLA
                     SRYPICSIEDGMAEDDWTGWKQLTDALGRKLQLVGDDLFVTNPLRLRRGLDEGIANSI
                     LIKVNQIGTLSETVDAVHMAKHRGYTAIISHRSGETEDTFIADLAVALSAGQIKTGSA
                     SRSERIAKYNQLLRIHEQLGTAARYAGRAAFPSLG"
     gene            11238..12623
                     /locus_tag="PKL17_03900"
     CDS             11238..12623
                     /locus_tag="PKL17_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43901.1"
                     /translation="MKADGAADDFSVCAARNLRYGRPALQPARSVPENPTSTLSPQME
                     RALLQLQLRVFALTWVSYASYYFTRKNLSVVKSRLHETLDISTTALGTIETLYLAAYA
                     LGQFLSGALGDKIGARKLLISGMLGTALAAFVFGASSTVVPFALAFFVNGLVQSSGWS
                     GNVKAMQPFFSSQSRGRVMGIWTTNYQAGGLLATALATYLLTHFGWRMAFFGPAIWVV
                     VVAVLLWLFLVEKPEQCGLPPVEPDVALRQGATPSSSESATGPTLFQLLRMNVLWTLG
                     LSYFGLKLIRYSLLFWLPFYLKQHLHYSESLAGYLSLPFEIGGMVGSVAVGWFSDRYF
                     RKQRLRVTVPALLLLGCALVLYQFLGGKGLWINAGLLACVGFFLFGPDSLLSGTMSQD
                     IGGRAATGRVAGIINGMGSVGAIFSPLIVARVSETFGWSALFFGFCGVTIVAAALLLV
                     SALLVEQQQRH"
     gene            12724..14844
                     /locus_tag="PKL17_03905"
     CDS             12724..14844
                     /locus_tag="PKL17_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43902.1"
                     /translation="MKRRSYSLVPTLVGAVLFVTSGLTGCSTEQSKCGSGLSECNGEC
                     LNAKTDPNNCGACGNSCASPLVCSSGSCSDSCAGTQVACGRACVELASDALNCGKCGN
                     KCENGATCTDGKCTGGNQPECKDRIPFATSGSYDLDAKVVQVSGKLTKNGVVVPDGSY
                     RGNLTFESKTTGTRSMPSFKDTGDATYQGSVFAGTYDVFYNPSGNCSQTGPLPCQRTL
                     LRSGVNLNVSGSLDFDVKTVQVSGKLTKNGAIVPDGSYRGSLVFESKQGGNRSMPSFK
                     DNGEATYQGEIFAGTYDVRYQNSGSCSDRSSLPCQSIVIQEGVNLTVSGALDFNVKTV
                     NLSGRLTKNGAMVPEGANRGYLSFELKQGSARTMTSFKDMGEATYQGEVFVGTYDVVY
                     RNGYYCSAGGVLPCGNLRLKSGLAVTVSGALDFDVKTVQVSGKLTKNGVAVPSGTNRG
                     YLRFEQSDGNSISMQAFKDTGDATYQGEVFAGTYDVVFEPSYYCNSSSPLPCQRLRLR
                     KAVPLTTSGSLDVDGKTVNVSGKLTKNSQSVPDGTNRGSLSFAHKDGGVVSMLSFKDM
                     GDATYQGELWAGNYDVSYLPSYYCGTSSPLPCQSAQLRTDVQLTVSGALDFDAKTVNV
                     SGKLTKNGAAIADSTYRGSLTFTEKSGSSRTMPSFKDTGEATYQGDLFAGSYSIFYNP
                     SYNCSMTSPLPCQTTLLVGCPAAK"
     gene            14878..16362
                     /locus_tag="PKL17_03910"
     CDS             14878..16362
                     /locus_tag="PKL17_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43903.1"
                     /translation="MKIKNLILGTLCALLASCTEPNPPAPEDMMTPWDGNTEGCMLGQ
                     FRCGSGCVDVQTSIDNCGRCDNKCGDRQQCVGGACKDQVGDCRKDGGCSKGYYCDLNS
                     GACKQGCGANPDCGMNEACDISTHKCTCDSNSHDCAGKCVRSDDVKTCGTRCDACPTD
                     PNGMALCYLGMCDVQCKSGFRECSGVPGCSECCRDYDCPFGSGKICASNKCVVATRCT
                     KTSECGIDEACTTGICTSTAPGGTCTTTADCPNGQMCQSNKCAVVSCPAESHGFANSA
                     CPVGAGCRKARCVNLIDASCSTWSDCGNDYTCDGATGTCKLITGSRDCTTNYNCTGYG
                     YGYACVNGHCQNVGASSYCQADVECGFGAYCGRINKTGRTNYCTGMCSGTGYPSCTTG
                     YSNTCSTVRCKAYGSSAKGCCSRIHNTCTYDSDCNGYKCVSGRCASSASCTSTASCQF
                     AEKCESNVCKFTPSRTCTADNDCGNGQVCMSGKCGPEFEASGWN"
     gene            16477..16818
                     /locus_tag="PKL17_03915"
     CDS             16477..16818
                     /locus_tag="PKL17_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43904.1"
                     /translation="MVRSSAQFDSLSDARAQHEKLIEALDRHGRQGRFMLTDLREAVP
                     RNDPGWEALMAELRPRMTRGYRRMGVLTKTAAGTIQVSRHSRQDGVEMLLSDTEEQIL
                     AFFRIAAKMTR"
     gene            complement(16879..17343)
                     /gene="smpB"
                     /locus_tag="PKL17_03920"
     CDS             complement(16879..17343)
                     /gene="smpB"
                     /locus_tag="PKL17_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNK43905.1"
                     /translation="MSSPKGDGRKVLLRNSRARHDYFIEETMEAGLVLVGSEVKSLRA
                     GKASLTEAYAQAKNGEAFLFDMQVSEWSFANRFNHEPRRERKLLLHKAEIKKLDEATS
                     QKGYSLLPLEVYLKDGKIKVLIGLGKGKKQFDKREDQKEKDAKLDMKRAMGR"
     gene            complement(17359..18531)
                     /locus_tag="PKL17_03925"
     CDS             complement(17359..18531)
                     /locus_tag="PKL17_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNK43906.1"
                     /translation="MTIEIPPFSSNVDSAETLPSWLYTDPAVFELERERIFSCTWQPV
                     GRLEQVETPGSYFTADVAGEPIVVLRDSDGILRAFYNVCRHRAGPVAALCGKRQTFTC
                     RYHGWTYSQSGALRSAPEFDDVPCFRKEDFGLRPIAVDVFGPLLFVNLAASPMPLAEM
                     IDGIPQATERFKLARLRLCERVEYEVACNWKVYIDNYLEGYHIPVVHPSLYRELDYAN
                     YRVETARYYSSQIAPLRKRNDPSGSGKERLYSDATGDAQALYYWVFPGWMLNVYPDNL
                     QCNAVIPLSVSRTRVVFEWYMLTDGAMVNRTLPEGIAHSPNDAREAIRKGIEFGDLVQ
                     KEDIEICEAVQKGLGSRSYDRGRFCGKRENGVHHFQSLVHEFLFGPQKARDEVTPR"
     gene            18698..19534
                     /locus_tag="PKL17_03930"
     CDS             18698..19534
                     /locus_tag="PKL17_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNK43907.1"
                     /translation="MQTVWKTLWTLAASALFSASCTPEFPTVTGTVSVEPLHSKLVSD
                     DYLLRVRLPPSYETDPTRRYPLIVQLDPTFVGLHEFDITCGLVSEHAGEGRWPEAIVV
                     GPDYPDPNTRLRDYVLPKPLTESFSGQNVDLFYRVMKEEILPYVEAKYRIDSTKRILI
                     GHSAGATAVYYFTFRHAPPEAPLFSGAIAADDGIEEGFFTLERWHAMRTDSLPIKLFA
                     TRALWNGAVQEVPYRALVSRIQSRNYQGLSFAEKTLDTDHGGAIYPSFEQGLDFIFGG
                     GK"
     gene            19531..20184
                     /locus_tag="PKL17_03935"
     CDS             19531..20184
                     /locus_tag="PKL17_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43908.1"
                     /translation="MNWAKVIPSIASGLLVSALSATSIAEEPSLGIPSGGGEHGHPVH
                     VRLDSAATYGIGGQSYLGIDLKMSGYLPVWDTRLGTGSIDAGVVLAYGNEPTWLAPWI
                     DTSQVHGATHRISLLARLGHTFHIGVRRRAALGFHLFGGLHEWISAYSVNYQSEGISG
                     SAALKRSLFVSGGELDFAYRFSDPVGLYLELSAPFPYQSSYAQTMFHVGAGLTFYLR"
     gene            20218..21234
                     /locus_tag="PKL17_03940"
     CDS             20218..21234
                     /locus_tag="PKL17_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018720020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HNK43909.1"
                     /translation="MRQVWITRPGPPEVLQVKESPDPVAAEGQVRVSVRAAGINFADL
                     VARTGMYQDAPPIPCVIGYEVSGVIDQLGQGVQGFSVGDRVLAMPKFGGYSDTVVLPA
                     NQVIRMPDKMSFEEGAALPVAYLTAHHMMLFVGNLRKGMRVLIHSAAGGVGLAALDIG
                     RAHGCEIFGAASGTKHAFLKERGCQHPIDSGKDIPSQVRAILGKESGLDLVLDPIGGK
                     SFSDSYNLLSETGRLVCFGVSSMSPGKSRSFLSALGMLIGTRRFSPLSLMMDNRSVQG
                     VNMGHLFSRLDLLLPQFESLVAMYERGEIHPYVDRTFRFEEAAQAHHYLHDRKAKGKV
                     LLVP"
     gene            21263..21670
                     /locus_tag="PKL17_03945"
     CDS             21263..21670
                     /locus_tag="PKL17_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNK43910.1"
                     /translation="MFVPPHHPYFDDRAAMLWHRSFDEAFAQAKQSGRRLLIVVTHEE
                     CGGSRALLERVFPKEEVTHELRSGFVCLAADLHRHEPQVAELLAQAPRREPTPVCLYV
                     AAEEKGPRLLLSTAGGRPPAVFVRDLTDAHARK"
     gene            complement(21695..22591)
                     /locus_tag="PKL17_03950"
     CDS             complement(21695..22591)
                     /locus_tag="PKL17_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43911.1"
                     /translation="MTRVLSGDRATQWGRALFACALYAFGLGAVLFGLVHKKRPAIPP
                     VHALERARERGLIGDLIPRLDPQDAFANAAVLSALLDEVPHPRLSTGSDCQARARLLV
                     VMLDGRLSRVRFSGRQVCQLRRLKEVSTGQEGLAKNEVAEVAAFLLGKRLGDDVDVTR
                     VVVPPFRFSGDTLDFIAADLGPLSPGEQLIGTYHTHPGSELEQGVLSETDLAYMVSGR
                     AEIGGQGASLNDPGPQTDWLIDIVDPKWGDWNAFAHDRRKLKALHARCMASSPCPLNE
                     LRLGGSDNNLFVRFYEELPDEE"
     gene            complement(22711..23109)
                     /locus_tag="PKL17_03955"
     CDS             complement(22711..23109)
                     /locus_tag="PKL17_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43912.1"
                     /translation="MTRSFSVRKQRAVRLCLPFVVGLCGCHAAPAAPPLGTLVLGVSP
                     ATATVFLDEQPLLLRQTGGQPARVRMPAGPHRLEVRAVGYLTRFYDLAVRASADSVLS
                     VSLHPDPEADLDTQSAATTPFAPRPLPIPP"
     gene            complement(23106..24239)
                     /locus_tag="PKL17_03960"
     CDS             complement(23106..24239)
                     /locus_tag="PKL17_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43913.1"
                     /translation="MQVCFRHARLVLGLALFAILLGGGLRSACAQELQVETAVQVALT
                     DDASKTRNQALSDAMGQALLQAVEQSAPELRGRLYLVAPRAREFVSSYRVVEETEQDG
                     RLLFKIVSQVDVARLLRELQTALPKPKHSDGKPSVLLCVKLAMAEPLIEKLQASAREL
                     LGQRSLNVELGNADRCGPGSTWPGMRLSFEGNLESAVAEVRGTTPRLWSAHVRGVWQF
                     VVAPGAPPQQEAGDGVAFAERDDAALDQAVGQVGRPLLTKLAERTGLLGRPGGGVLLL
                     ATGLRTAAVMNKLWKALLALPGVSRVEPRRIVFSDGGDEQVHFQLMTQTSTETLGTAL
                     YRTPVAGLRMQVVPLGPSALRLDCVPAQDLPSAEVPEAEAAAP"
     gene            complement(24241..24702)
                     /locus_tag="PKL17_03965"
     CDS             complement(24241..24702)
                     /locus_tag="PKL17_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43914.1"
                     /translation="MRSWFFGSALLVSAAVAAHAEKPQTVAAGVQVDFAQGRLMAVAS
                     CAADLYPPSADVARLKAERVARSRAEDKLRKALPLLVRQHKAELSRFGGADQVAKLDP
                     KTATVQRIDYGATGSVVLWLALPLAASKERATSDSATAGAPDAGASPSEEH"
     gene            complement(24699..25640)
                     /locus_tag="PKL17_03970"
     CDS             complement(24699..25640)
                     /locus_tag="PKL17_03970"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK43915.1"
                     /translation="MAASHAALRGGDPVALRMSDGVIICGEWFRGNEAPRAVVLLSHA
                     MMVDRRTLDRPAGQGLLSALLAAGLWVLWIDQRGHGQSQPGPSLGARWDYDTLLFDVA
                     ALAAYLQQVAPDVPRVAVGHSLFGHLALAWQARSFDVAGAVRFDGLVLLAANVWLRRH
                     ESWRFRWLAKKLSYSALLWAARPLGYLPALRLGIGSDDEPYAYLAQMGSWVFSGDWAD
                     RHGHSFLAQLPRVKEPILSVAGAGDRLMAIPGCQLRFVSETSGPITHWQVGRRFGDPI
                     DPDHMDIVRSSTLRPLFDDIAAWICTPGRFDKKGGAL"
     gene            25753..27138
                     /locus_tag="PKL17_03975"
     CDS             25753..27138
                     /locus_tag="PKL17_03975"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK43916.1"
                     /translation="MAKLFYRDVSGTTGSIELGSVPVLIGRATDCQIQTQDAQVSRRH
                     ARVIFDGSGYFVEDTGSANGVFVNNERVPRYQLRHGMTFRCGHLEARFESEAAAAPAA
                     PVAAVPPPPPVISAPPPPPPAAVASPPPPSPPPPAAAAPPPPPAVPAQAAELASLRSE
                     LESERRKRTDLEFEITELKRKLDETQARPAATSDSDSEKLRRRVEQLESELKRKGSAA
                     APTGGGAPSEAQKAAEAERDRLRTRVAELEAKSKEPVKNDDQEMELIRLRRKVEQLEA
                     DLRRIRGGKPAEPAPAPAPTGDALVAELEEKVRRLISERDEALQKASAPVLVAAAAPP
                     PTDPKIAEELEKAKRRIDQLESELRRKPVGQVADTQRATEQRTELESALRQLRDVEKE
                     RDSLRELVARSSGSGRPAKATLDGIQTVSDGLADIRAALRAAGDDVALEQLEQLRTTL
                     RQVCSALGLTT"
     gene            complement(27160..28110)
                     /locus_tag="PKL17_03980"
     CDS             complement(27160..28110)
                     /locus_tag="PKL17_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNK43917.1"
                     /translation="MRDFLYRVFVEDLLLKAFALIVAVGLAIFVRTELEQSTTLYVRV
                     NYIEPRGRIMVSDQKLSEVRVTVRGPWARISRIEDTALQPIVIDMSNLSDGELRFSPE
                     MVRLPPGLRIESFNPVGMYMHFEPEALVSLGVNLTIEGEPPEGYRFKQATPTPRLVRL
                     RGAQNVLESMHNVLSRPLSLVNVRDTVTVPVELAPLPKHVRLADEPPPKFTATIVVEL
                     VERHYASVPITPNGQPQNVRLNPTTAMVILRGQGLDRFLDVPQLMLDTSPDERKPVGS
                     VFHKRPQIVNLPPGIAYEIQPAEVEFTILRAEPEKPGRNK"
     gene            complement(28116..29270)
                     /gene="cdaA"
                     /locus_tag="PKL17_03985"
     CDS             complement(28116..29270)
                     /gene="cdaA"
                     /locus_tag="PKL17_03985"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNK43918.1"
                     /translation="MSVFAPMWLSLRTLAAAINWREALIACADVIIIFYILYRALKLV
                     RGTRAAQAMTGLALITGLYFLAKRLDLVAVSWLFENLINYFIIFFVVLFQQDIRRALM
                     QVGQNLFMRRQYEETYVIEEVVQAAGELARQKIGALIVLERDADLTDHLGEVGQDLDA
                     KVTKELLVTLFVPEPANHLHDGAVIIRNLRVAQAGAVLPLSGNSKLDKELGTRHRAAV
                     GVTEETDAVVVVVSEERGTISVCFSGNIIRDLDALNVRKALLGLFQKKKNKSKDRPTG
                     RTGKADRSANDISLGGVPLPSMGSRPSNDAMPTQPLPVEPVPSTPVAPAGVAPSVHEL
                     ATLPIPADRGAVAKKPVELDGDTHKLPAIRPDDLAAQPAPDKDGAKAKTE"
     gene            complement(29267..30220)
                     /locus_tag="PKL17_03990"
     CDS             complement(29267..30220)
                     /locus_tag="PKL17_03990"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNK43919.1"
                     /translation="MIANHQNPVFLRPEIAPLRLQRSDGKWLDLDGKTCAVMGIVNLT
                     PDSFSDGGKLIDPRTALRHVETLVAQGAQLIDLGAESTRPLAQLLSAEEEWQRLFPVV
                     SELPRLGLPAVLSIDTRHAEVAARLLDLGFRVLNLAFPQHLFSPELDSPGAARLSHLQ
                     RQRLLAGFDAIVVMHSRGTPANMRELTDYDGDLCDTVCAELSLAARMLTDDSPSLLQR
                     IIYDPGIGFAKTAEQSLTLLRSTERLKAQLERPLLVGCSRKSLWGTLTGQPVDSRLIP
                     SLIGAVVAAQAGASIVRVHDVAETKLALVTHAALSRGRPGS"
     gene            30342..31451
                     /locus_tag="PKL17_03995"
     CDS             30342..31451
                     /locus_tag="PKL17_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK43920.1"
                     /translation="MNRSVSAKYIDPLDHIWISAAKELGLKVQRSPHGYAHTDGKGNL
                     YFAPPDELDDDDCLAQMIFHELCHALVQGEGSFDKPDWGLSNDEQADVYAGDVTREWA
                     CLRVQASLLRPYGLRRLLAPTTDFRAFYDKLPRHPLAEAPEREAALAQAGLSLAHRPP
                     FRRVLSDALIATCELHRAVSKSARQPTNPKAEPHEAPLPILWHSVPSPKLHKSGLPMS
                     DGMFEAPRGTTCQDCAYCLPPEKRGASHRCQRTAVDDTPGKVVKETTGACALFEPPLD
                     CQRCAACCRHAFDLVPVRTTDEITWKHPKLVGKAGKDLTVLRDPVRRCCAALDGSFSD
                     GYGCLIYSERPRTCRDFTSGTFNCLEARRRVGLDA"
     gene            31479..32324
                     /locus_tag="PKL17_04000"
     CDS             31479..32324
                     /locus_tag="PKL17_04000"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNK43921.1"
                     /translation="MTYTSFSLSVSDHVAQLVLCRPDKLNSLTIEFFQELLDVCRALE
                     RRSDVRVLVISSTGKHFTCGLDLMALGSLAADLGHGERSRAAYTLRTCVHELQQSISA
                     VEALRIPVLIAVHGGCVGGGVDLATACDLRYCTADSFFCVQETNVGLVADLGTLQRLP
                     KLIPDGIARELCFTGRRLPADRALALGLVSAVFPDHETMLREVNKTAQEIASKSPLVT
                     AGIKDVLHFGRDHGIKDGLSYVAVWNAGMLSAQDLMEQAAAKGAGRPARYDDLPAPKD
                     LVKVR"
     gene            complement(32321..32761)
                     /locus_tag="PKL17_04005"
     CDS             complement(32321..32761)
                     /locus_tag="PKL17_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNK43922.1"
                     /translation="MTAFLALPAAKPLIVFSVLLVLKMVGVAFATANARRKAGVVVNP
                     EDTGVNPGSHVEAQDAPDVLRAKRAHQNDLENIPAFLFVALLFTLTGGSATGGWAYFG
                     LYFAARLFHTIMYLAQKQPWRTVCFALGQITLLGLLAQLVLKVL"
     gene            33041..33655
                     /locus_tag="PKL17_04010"
     CDS             33041..33655
                     /locus_tag="PKL17_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43923.1"
                     /translation="MNSTDGGGTDDMAVAPLDLAMPDLSMPDLAIPNDPHIPASCTGA
                     QLNLASKFPMGASYVVLANYTMKYEQRSCTDFTGCGSWGPSTPSWGPSGSGKVYLKVT
                     GTGIIVAVVDNSAGSAYGTPVYNLGMDCALSAGVWNCGRYHDTEIVEGANFLKFEGKD
                     KFSGVELPVFGEVLANCARFYGTVQGVKTAGSYKEYRGGFLLRY"
     gene            complement(33656..34999)
                     /locus_tag="PKL17_04015"
     CDS             complement(33656..34999)
                     /locus_tag="PKL17_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK43924.1"
                     /translation="MTSQPLPESSRIAAYNALQYLSSPYRFYERAFGQHGDTFLFHGP
                     NGPVVLTRNPEHVKTIFTLDTSQIGIYPEDGIDVVTGPNSILVITNEKHRRERKLLGP
                     PFHGARMRAYGQVMQKAVQQEMRSWREGEVVDFQGAMQRISLSVIANAVFGLADPEES
                     ARTQKLAVTYMESLSPLMLFFPALRREYFGFKLFAKTYETRREMENALAARIAESRSR
                     PPSEDILSMMIHAHYDDGSTMSDAQIQDELFTLLLAGHETTALGLSWMFYHLYRNPET
                     LVKLRQELHALGPHPEAESIAQCRYLDACANESLRLYPVVAETFRKLRVPIELGPYTV
                     PAGHAVSVSAIGVHHNPAIYPNPQRFCPERFLEHKFTPFEFVPFGGGTRRCLGAAFAN
                     YEMKLVAHEILSHAELALANDKEIKPVMRGATFGPKGGVPMRVLRLSLPQAGLPN"
     gene            complement(35234..37840)
                     /gene="secA"
                     /locus_tag="PKL17_04020"
     CDS             complement(35234..37840)
                     /gene="secA"
                     /locus_tag="PKL17_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNK43925.1"
                     /translation="MKESIERYVQRDLHFAIVDEVDSILIDEARTPLIISGPAEQSAQ
                     LYYKVNQIVPRLKKDIDYTVDEKHHSAVLTDSGVEKVEKLLGIHNLYDAQNIEWNHHV
                     QQALRAHTLYKKDVNYMVTDEGKVVIIDEFTGRLMPGRRWSDGLHQAIEAKENVQIEE
                     ETQTLATISFQNLFRLYKKLAGMTGTADTEAEEFHKIYKLDVVMIPTNLSMVRKDHPD
                     LVYKNERGKFRAVIEEVAECYERGQPVLVGTVSVEKSEVLSAMLKKKGIPHNVLNAKA
                     HAREAAIVAQAGASKQVTIATNMAGRGTDILLGGNPEFMARTEIYGEEMACRPGACDE
                     TAPEYKEAFAKHKKTTDEDKKKVRELGGLHIVGTERHESRRIDNQLRGRAGRQGDPGS
                     SRFYLSLEDDLLRIFGAERITGLMERLGMEEDVPIEHSMVNRAIENAQRKVEAHHFDM
                     RKHLLEYDDVMNQQRKSIYALRRSVLEGRLPGFDVEAILGGKGIPVPAPKDSADSDEH
                     VAVTAPSVDKVKAEYEGWDKRLEPVVKVIVDAFWENPEGEAAEAGSHFGDKPGEIAKA
                     SGKSGKLRSARDLTHELYRRFGSVIEFGSEEQTDKDKAVKKIVHETAVSLVQQRKRVF
                     DLAYDLVEKIVVEYCPEDKTPDDWDMTALAAAIKDQFGVAVTLTDVNLEVGSIADMAW
                     EQIEKYTESREAELGLAFLYFARHFWLEEIDSQWIDHLKAMDHLREGIGLRGYGQKDP
                     KQEYKKEGYNMFMQMTDTISQNVAKKLFLVRLDQQAQPETPEQEAEQKLPEFKHKARR
                     VVLQHQSSATSGAEDEKSTRDISRDDQAEVEGADPKQKTAKREEAKVGRNEPCPCGSG
                     KKYKKCHGANAA"
BASE COUNT         7469 a        11856 c        11047 g         7488 t
ORIGIN      
        1 tcggtaggtg gcgcaatctt gagttcctgg gccagcttct ccccgaactc gcgacgacga
       61 acgtcgagag gcttgtcgcc gggtggctcc cagcccatgt tggtaatctg gtccagcgga
      121 cgcagccgcg gggccggatc gcggtgacat tccaagcacc agcccatcga aagcggctcg
      181 acctggcgaa ccacttccat ctggtcgatg cgtccgtggc attccacaca gccgacgccg
      241 cttgtgacgt ggcggctgtg cgggaaaaat gcgtagtccg gggtcttgtg aacccgaacc
      301 cacggcaccg gcttcccgta tgcccacgac tcgcgcagcg gctggagctt cggcgagtcc
      361 ttccgcacgt tcgagtggca gttcatacag acctgcgtcg gcggtacggt tgcgaactcg
      421 gtgcgctcga cgttgacgtg gcagtagcga cagtcaagtc ctagctcgcc agcgtggagc
      481 ttgtgagagt acggaatggg ttggttgggc tcgtacccca cgtccgtatg tcgtggggag
      541 aagtagtacc agacgccaaa aatggcgcca acgagcccga acaaaacggc ggccacagaa
      601 agggcgggca accggttcgt ccacggaggg aagacaaagc gtttgtcaga tgtcggctgg
      661 ttggtcatat cgggacgcct ctacaggaat cttcacggcg ggcgctggcc agttgatcgg
      721 cgatgcacat accggcgcgc agcctatcaa aagggcccac gagaaagcaa ggtttggatt
      781 gagcttggaa gacggcgggc gaaactatag aaggctacac ggcttttggg cgtcgacacc
      841 aacgcgccca ggctgatcag cagccggtag agacaacgtc accggatcat cgagatcggc
      901 ggtagatgta ttcgaagatc gcgggttgta tatctcggcc tcacttgtga cttcagtggt
      961 ttgccccatt ttcctggtca atccacctgt cactaagatg ccgccgtctt gcaaaagtgt
     1021 ctgctgatga ccaaatcgtg caaccgccat cgctgggagc cgcgacaagc tgcggctggt
     1081 tgcatcgtaa agcgaagatt gggtcagcgt acaaagtagg ctggtatccg atgcggtctc
     1141 acagtcattg cagcccgcaa cttgggttgc actgccaccc gtgatgacaa cgcgccgacc
     1201 tgacgcggtg gccgaagcgg cctcatacgc tgccggacgg tagtgcgcac tgctcgatcc
     1261 gcaggttgcc acctgatacg gcgtaatcgc agattcgttc ggcgcactgg tggcgtcttt
     1321 caccgtatag acgcgcaccg cattggctgc acttggcggc tgacccggcg aaggggtcgc
     1381 ggaatcggtc aactggaaac cgccagtgac cagcacatcg acaggaaacg ccggactggc
     1441 gtcgagcggc ccgccaatcc gtgtcgccgt gtgaaacgcc gtcgccaccc ctgccacact
     1501 ctgggccatc gccgtctggg ccgccggcgc ttcgggcaat cccgtcagca aaagcgcagt
     1561 cattggcggg gtcatcgtcg acgatgccgg ccaagaccca ccgagcgcca gcgccgaact
     1621 cttcgatagc agcgtcagcg atggagccaa aaacccagat gagaacgcac ctgatccaaa
     1681 cacctcgcca cttgcgcccg accaggccac cgctttttgc accgcagtgc ccgccgtcgg
     1741 cttgagcaaa tcgacaaaga tgctcatcgt gcatggcggc gtcgacgaaa acttcgcgcc
     1801 ggctacgctg accagaccac cttgcggcag cgcggtcgtg cctgcgaacg ccgccgtatc
     1861 ggtctgaccg acggcttcgc ttgtcacagt ccacttgcct gcatcaaact tgagcgtcag
     1921 aagctctggt cccgctggcg ccgcttcgcc gcacggagcc agccgcagag gcgcattgtt
     1981 gaacggcgtc gtcagaactt gtcgtccgcg cggcgcagtc acaccaccga acaccatcag
     2041 cttgacctgc gtgtccgtcg cgctcagcac ggcaacctga tgaaacgccc gcgctatcgg
     2101 tccctttcca gcaacggaaa ccgtctggaa cgtggctttt gccgggtcgt acatttcgac
     2161 ggtatcaatg acacccatgc tgtcggaaat gcccgcgacg acgctggttt cgacgccacc
     2221 gtagatcagc acttcgccgc cgggcagtgc cgttgccgaa tgaaacgcgc gcggccccac
     2281 catccccgat gccggacagt tccagctccc gacttcgaac atcggcaaaa tgccggcact
     2341 tccgtcagca gccgcgctcg atttgccctg aactgcgccg gtgtagacgc gcttgccgct
     2401 tgcatcgaac agctcggcaa aaaaagcgta ctggctgcga tcggtggtcc caaggtcaat
     2461 gcttgggtgg tcatcgggga gcgtggcttt gatgtcgcac tgaaactgcc agccagtgcc
     2521 ctgtttttgc aacaccgaca gacggaccgt tccgtcgcgg aggacggcgg aaagcggaaa
     2581 tccgctcttg tcggtcttga tgcaggcgtg ttgactggcg gactcggcga tttggagctt
     2641 gagccccgga ccgggattgc ccttgcagct taccagccca agaagtccta acgatagact
     2701 cgcgacgcgc agacgataga agcccattac attacacggt aacgcacggg cgtaagagcg
     2761 gtcaatcgcg cgaggaaaaa cggagcagca gccgcagcgc ggtgacaaag atgttaaaga
     2821 gcgccgcaaa cagcatcagc gcgccctgca ccgggttgtc gtagctcggg ttgttgagga
     2881 catcgctcgt tgcatagacc aagctgacca cgccgaagat gccaatcccg atcgtgatca
     2941 gaaggcccag gcccgatagc tgaaggaacg agtttacgaa catcgcgatg atcagcgcga
     3001 agaacatgcc cgtcagaaga ccggctggcg ccgaaaactt ttccttcgaa aacatcacgt
     3061 agccggtcac cgcgacaaag acaccgccgg tcaccgcaag cgccgccgga atcgtgcccg
     3121 gcgacaagta gttggcgatt gcaagcagcg gcccgagtgc aatgccagaa atgaacccgt
     3181 cagcggccag cgcgacaatg cccaggctcg ggctctgacg cgacgcccac agcgcaaagt
     3241 acggcaccac attgagcagc acgagcgccg aaatccagcc caccgggcga aggaaaaacg
     3301 acagcatcgc ttccgagtgg gaacacgaat aaccaccgac ggacgccatc acgacgctga
     3361 tcagcaagag cgcgtaggtt tttttggcca aaagcgcccg atcaccgtcg aggatcgtcg
     3421 taccaaacat tttttcggaa aaggacagag tcgacatggc ttcactccat gtgggggtta
     3481 cagcacgttt ctgttcatta tgagcacggc catggccgcg cgaaagtggt gggggaagct
     3541 atacactacg gcgactcacc gcgcagcagc ttctcgatcc cggcttcgtc cagaatcggc
     3601 accccgttct tttcagcggc ggcccgcttg tcttcaccag gttcggcgcc catcacaagg
     3661 tagctggttt tcttcgtcac cgacgacgta aacttgcccc ccgcggcttc gatgcgtttt
     3721 tggaagtcgc cgcgcggttt cgacagcgtt ccggtgatgc aaaacgtctt gcccaggagg
     3781 ggccccgtcg aagccaccgg ctcgactggc gatagcccga gcgctgcgaa cttgtgcaac
     3841 agcgcgcgcg cctccggatc tttcagatag tcgcgcacgg caccagctcg ctcgtcgcca
     3901 aagccgtgga ttttgaccaa cgtgcgatag acatcatcgg gggccgccgc gagcagcttg
     3961 gtcagcgacg gaaactgctc ggcgattttc tgcgcccaca cccagccaat gccgggaatg
     4021 ccaagcgccg tcacaaagcg cgacagcgtt gcttccgctt tgccggtcgc aatcgcggat
     4081 atcacattgt ccgcgctctt ttcgccaagc cggggcagcg acagaagctg ggccttggtc
     4141 agcgaaaaca gatcggccag atcacgcagg agcttggccg ccaccagcga ctcgaccagc
     4201 ttgtcaccaa gtccggtgat gttcatcgcg tcgcggttgc agaaaaacgt gatcgcttcg
     4261 cgaagctgcc tggggcacgg cttggtgttt ggacagcgca gcgcgacttc gcccgctcgt
     4321 ttgatcagcg tatcgccaca ttctggacac tgcgtcggcg gcaaaatggg cacaagctcg
     4381 gcctcgcgcc cggcccgctg ctgtttcacc actgcgacga tctgcgggat gatttcgccg
     4441 gctttttcga ccagcacaaa gtcaccgacc atcacgccga gccgtgcaat ctgatcccag
     4501 ttgtgcatcg acgcgcgcga caccgtggtt ccggccagat cgacgggctt tagcaccgcc
     4561 accggtgtca ccacgcccgt gcgtccgacg cccagctcca catccagcag cttggtcgtc
     4621 gcctgctgcg ccggaaactt atacgcaatc gcccagcgcg gcgcccgcgc ggtaaagccc
     4681 agcgactcac gctgcgccag gtcgttgacc ttgagcacca gcccatccac gtcaaacggc
     4741 aggctcatcc gcttgtcggc ccagacttgc acctgcccga gcagcgcatc cagactgccg
     4801 acgatctgcg tatcgggata gaccggcagt cccagccgtc gcagatagtc gagaagtccc
     4861 gcgtgcgtcg caatcccagc aacttctccg acgacttcat acaaaagcac ccgcaggggc
     4921 cgcttggcac agatccgcga atcgagctgt tttagcgtac cgcccgtcgc attgcgcgga
     4981 tttttgaacg gttcttcccc cgccgcgagc cgctcgacgt tgagcttttc gaaaccgtcg
     5041 cgcggcatat acacctcgcc gcggaccagc agatcgactt tttcttttag catcatcggc
     5101 aggtttttca ccgtgcgcag gttctgcgtc acgtcttcgc cgataagtcc gtcgccgcgc
     5161 gtcgccccga gagtgaacac gccggctttg taggtaagct cgatgccgat gccgtcgatc
     5221 ttgggctcga tgacaagctc gggaggccgc ctttcacctg ccccatgcag cccgcgctgg
     5281 acccgtccga aaaagtcgcg cagatcgcct tccgaatagg tgttgtcgag cgacagcatc
     5341 gccaccgccc gttcgacctt ggcaaagctc gacagcggcg tgtgtccgac gcggcgcgac
     5401 ggtgagtgcg caaccaccca atcgggatgc gccgcttcca gcgcgtcgag ccgcttcttt
     5461 ttctggtcgt actcgtagtc ggcgatgctc gggttgttgt cgacgtaata gcgacggtcg
     5521 tgttcgagca gctctgccac cagcgaaagg tattcttcgc gactcccctc gtcgccaaac
     5581 aagctgccaa ccgctgccac tgtcgcggcg ggcgacggct tgcttggacc ctttttcttt
     5641 gcgctcacag acggctcctt cggttcgttt gcagcgacaa gcccagatcc tgggccacgc
     5701 cgcagtttcg ccatgatagc agcctctgcc aaaagccggc acgctcactc tgtcgccggt
     5761 tttgccgcgc tccgttccga cggtgactca tcggatttgt actcgctgta gtgaccataa
     5821 tacgagcgat agcctttgcc gctgacatcg atcgcgttta gcaccacccc gacaatccgt
     5881 ccaccgacgt caagcacatg ctgcgcagcc cgcgtcgcgg cctcttttgg cgtttgtgcg
     5941 tgacgaacaa tcaagacaac gccgtcgaca agcgtcgcca aaatcgccgg atctgtaacc
     6001 ggcaccgcag gcggcgagtc aatcagcacc cgatcaaacc gccccgctgc atccgccaga
     6061 agctggcgaa agtgctggcc gctcagaagc tccgccggat tgggtggcgt cgggcccgca
     6121 ggcagcaccg acagattttc gacttctgac gaccgaatcg cttcgtcgag cgacagctca
     6181 cccaccagca cattggtcag gccgcttgta ccacttaaac caaaggcccg cgcaatccgt
     6241 ggccgacgca gatcgcagtc gatgatgagc gtcttttggc ccgacttcgc gagcaccgtg
     6301 cccaagctga cgcagcagag cgtctttcct tctcgcggca acgacgaagt cagcagcagt
     6361 gtcctgagct gctgatcggc accggcaaac gtcaagttgg tgcgaatcgc ccgacacgcc
     6421 tcggccaccg tcgagcgggg atgataggca acatacaaat cgggcggata gcgacccttt
     6481 gcattcccat cgacagcaaa gcttggcagc attcccaaaa gcggcagccc gaggctggat
     6541 tcgatgtcct gcggcgcttt caccgtccga tcaagcgcat caagcccgag cgccagcagc
     6601 accccaaaca gccccgccag cacaagcgac agcgccagca ccacccgaac ccgcggactg
     6661 accggaattt tgggccgtcg cgcatagtcg agcactttga tattcgacag acgcagccgc
     6721 ccgaccatcc cgctttcttt ttcgcggttt aggatcaggt tgtactgctt ctggaagctg
     6781 tccgcgtcgc gcttgagctt gttgtactcg atttccaggc gcgacagccg catgccttct
     6841 tgcttgacga tttgcagctc gttttggact ttctgttcgt cggacagcgc ttcgctatag
     6901 cgaatctggg acgccgacag cgcgacatcg acttcgcgcg ccagctcacg caggatttga
     6961 tcgactttcg acgtctgctg ctgcacttgc ggatgggtgt cttggtagcg ggtcttcagc
     7021 tcggccagcc gccgctgctc ctcggtgtag gcacgacgca aatcgccgag aatctgcggc
     7081 atgtcgcccc cgagcaccgg cacatgcagc gcgtcgagct ctttgagctt gcgcagctcg
     7141 gtcagctgac tttggcgctg gagcttgcgc agcttcacct cggtgagcgc gtcggaaaac
     7201 ttgtcgattt ggcgcgccac ctggttggtc cgatcttcca tgctcaccgt cagaagatcg
     7261 tgcttcgact tgaactcata aagtgccagc tcggcgccat gcagcgactt gcgcagacga
     7321 tccagctcgt cggcgagctg ctcgctggtc cgttggttta ccacttcgcg gctctgcgca
     7381 gcgtctgcaa cgaactcatc agcgacggca tcggcgattt tcttggccca gtctggactg
     7441 gtgtgccggg ctgtcataag cagcacccgc gtttctgcca ccacgtcggc actgtagtag
     7501 ctaagcagcg cttcgccagc ttcttcgatg gtctgcggcg gcgttccccc ggcgaaaaag
     7561 cccggctcac tgagcagccg cagtcgcagc gccacccgcc gggacaacga atccgacgtc
     7621 agcactttgc gactggtttg cagaaaatcc tgcatcagat agtagtttgc cggcgtcgga
     7681 tcgacgacat cctggaccgc actaccaagc acctgcggga cgttggcttc gacgagcacg
     7741 ctcgacttgg tttcgtaaac ccgcggctgc gatttcgtcc acagccacgc cgccgtcggc
     7801 cacagcacca gcatcagcag aaccgcccag cgccgttccc acagcacagc gacgtaacgc
     7861 cacagctcac tgccgctttc gtctaggctg cgacggtccg tgagcatctt tccacggcta
     7921 cgcgctcccg aatcgggcgg aagctcggtt tgcgaagtgg ggcgcatcgt caggccctat
     7981 tgtgccacca cttgctcaat ccgcagccac ggaatgcaag ctttctgcgt ctgccaagag
     8041 agcgcaaacc actgcgatgc tgtcgtcgga aacttcggaa atggcgggtt acaagtcttc
     8101 cgcttcgctg tctatcgacg tcgcggaacg atgccgggcc agggccagcg cgctcagcga
     8161 ttcggcactg cgggcaatga cttcgatcga gcgatggaat tcaccaagcc gtgtctcttg
     8221 cgcccgcgcc agttcgcgca gtgcctcgac ggcttgcagg atttgatcgc cacctttggt
     8281 ttgctctcgc tgcgccgtgt gaagatgcga caccatctcg ctgatggact cgatcgagcg
     8341 ggtgatttgc ttgctgccgc gcgtctgttc ttgtgacgac cgctcgacgt gcttggtgat
     8401 gtttttcatc cgctcagcgg ccttgagaat ctgttcggac ccgcgggctt gttcgctggt
     8461 ggcggttgca atctgctgca cggtttcggc gatcgaaccg atcgcatcgg tgacttgctt
     8521 gctgccgcgc gcctgctcga cggtggcgcg ggcgatgtcg cgaaccatct gcgtcgcacg
     8581 ctcagcggct tcgagaatct tgcgcagcgc ttcttcggct tggtggctga ctttgacacc
     8641 ttcatcgacg gctttggcgc cacgctcgac ggcgtcaatc gcgttgcgcg actccatctg
     8701 aacgctcttg attagttcgc cgatgtcttt tgtgcttgcc gacgaacgct ccgcaaggtc
     8761 tttgatttca tcagcgacga cggcgaagcc ctttccgtgt tcaccagcct gggccgcaat
     8821 gatggcggcg ttgagcgcga gcaagttggt ctgttcggca acgtcgtcga tgatgcgcag
     8881 aattttgtcg atttcgccga tcttgtcgcc cagtcgagcg atgacgcgaa cggtgccttc
     8941 ggaatagccc ttgatcttgt tcatgccgtc gatggtcgac gtgatggctt gggcgcccaa
     9001 accggcggcg cggctgactt cttccgacag acgagcggtt tcgttggcgt tgttttcgac
     9061 ctggcgaatc gaaatgtcca tctcgttcat cgacgagctg gtctcttccg cggtggccga
     9121 cagcgcttcg acgtttttgg ccacttcgcg aatcgagtag gtcatttcct cgatcgacgt
     9181 cgccgattcc tgcaccgcgc ccgccaggtt gaccatgttt tctgcgacct catcgttcga
     9241 ggccgccatt tccaaaatcg acgagctgga ctcttcggca ccgcgcgcca gggtttccac
     9301 cgactgggca atttgcttgg ccgacgccgt catttcgtgg agcgacgcac tggtttcttc
     9361 cagcgcatgc atttgctgcc cgatggtgtt gcgcagatcg gcaagtacgc cgttgatgtc
     9421 cccgctggtc ttggtctgcg ccccaagctg gcgtgacagt tcctgttggc gctgatgcaa
     9481 aatcttgcgc agacgttcca tcagctctgc gacattgccg agcggctcat cggcaccgag
     9541 cggcactggt tcttccagtt caccaccgac gagccgactc atcgccaggc gcagctctgc
     9601 gacctgactg ccagtccgca cctgaagcgt ggtattgacc gcgatgacga cgaccaccac
     9661 cgccaagatc ggccatgccg cagcttgtgc ttctttcgat acgacgaaca ggagcccgag
     9721 cagcagcgcc gccagggtga cgtttacgag caaacgccca cggacgatat ggttcatcgc
     9781 gcacagttta cagcgaaata aaaaggaaat ctgcctttca aatctttagg taacaaatca
     9841 aacaccgcgc agctttcgcc ggagcaagcg gttgaaagcg cgccgcggtc gtgcgatgct
     9901 gccgtcgctg acgttgaccc cctaaggccg ataaggagcc atccatgact gagatcaccc
     9961 agcttgtcgc tcgcgagatc ctcgattcgc gtggcaaccc gactgttgaa gtagaagtcg
    10021 agctgtcgag cggtgcgcaa ggacgcgcag ccgtcccatc gggcgcatcg accggaacgc
    10081 atgaagcact ggaactgcgc gacggagaag caggccgcta tctgggcaaa ggcgtgcgcc
    10141 gtgcgatcga aaacatcgtc gaagtgatcg aacctgcgat cctcggaatg gatgcggcaa
    10201 accaaggcgc gctcgatgca gccctgcttc agctcgacgg aacgccgaac aaatcgaagc
    10261 tgggtgccaa tgccctgctc gcggtgtctt tggcgtcggc ccgtgccgct gcccacgccc
    10321 acgagctgcc gctgtaccgt tatctgggcg gtatttctgc gcggcttttg ccggtgcccc
    10381 tgctcaacct gataaatggt ggggctcacg ccgacagtgg actcgatgta caagagttca
    10441 tggtggtgcc ctacggtgcg ccgtcgttcc ccgaagcact gcgggccggt gcagagattt
    10501 tccaccacct aaagaaagtg ctcaaaggcc agggcatggc aacttccgtc ggggatgaag
    10561 gcggttttgc cccggcgctg ccgcgcaacg aagccgcgct cgaagccctg atgaccgcca
    10621 tcggcaaagc tggttacaaa gccggcgtcg atgtcggcat cgcgatggat gtcgccgcca
    10681 gcgagttttt ctcggcggac aagggcattt acaaagtcga tggcaaagag ctgtcgtcaa
    10741 gtgagcttgt cgcttggtac gtcgacctcg caagtcgcta cccgatctgc tcgatcgaag
    10801 acggcatggc ggaagacgac tggacgggct ggaagcagct gaccgatgca ctgggacgca
    10861 agctccagct cgtcggtgat gacctgtttg tgaccaatcc gctgcgcttg cgacgcgggc
    10921 ttgacgaagg catcgccaac tcgattttga tcaaggtcaa tcagatcggc acgctgtcgg
    10981 aaaccgtcga cgcggttcac atggccaaac accggggcta caccgccatc atctcgcacc
    11041 gctcgggcga aaccgaagac accttcatcg ccgatttggc cgtcgccctg tcggcgggac
    11101 agatcaagac cggctcggcg tcgcgctcgg aacggatcgc aaaatacaac cagcttctgc
    11161 gcatccacga gcagcttggc accgccgcac gctacgccgg tcgcgccgca ttcccgtcgc
    11221 ttgggtaagc ccaagccgtg aaagcggacg gtgctgccga cgacttttcg gtttgcgccg
    11281 cccgaaacct tcgctatggt cgccccgctt tgcagcctgc gcgatccgtc cccgaaaatc
    11341 ccacttcgac gctgtctcct cagatggagc gggcactgct ccagcttcag ctccgggtgt
    11401 tcgcactgac gtgggttagc tacgcctcgt attactttac gcgcaagaac ctgtcggtgg
    11461 tcaaaagccg actgcatgaa acgctcgaca tctcgacgac ggcgctgggc acgatcgaaa
    11521 cgctgtatct ggcggcgtat gcgctgggcc agtttttgag cggtgccctc ggtgacaaaa
    11581 tcggggcgcg caagctgctg atatccggca tgctggggac ggctctggcg gcttttgtgt
    11641 ttggcgccag ttcgacggtg gttccgtttg cgctggcgtt tttcgtaaac ggcctggttc
    11701 agtcgagcgg ctggagcggc aacgtcaaag cgatgcagcc gttcttttcg tcgcagagcc
    11761 gaggccgcgt catgggaatt tggaccacca attaccaggc cggcggactc ttggcgacgg
    11821 cactcgcgac gtatcttttg acccattttg gctggcggat ggcgtttttc ggtccggcaa
    11881 tctgggtcgt cgtcgtcgct gtgctgctgt ggctgtttct ggtcgaaaaa cccgagcagt
    11941 gtggcctgcc cccggtcgaa cccgatgtcg cgctccgcca aggtgcaacg ccttcttcca
    12001 gcgaatcggc gacagggccc acgctgtttc agctgctgcg catgaacgtg ctctggacgc
    12061 tcggtctttc gtattttgga ctgaagctga ttcgctacag cctgctgttc tggctgccgt
    12121 tttatttgaa acagcatctc cactacagcg agtcgctggc cggttatctg tcgttgccgt
    12181 tcgagatcgg cggcatggtc gggtcggttg ccgtcggttg gttttccgac cgttatttcc
    12241 gcaaacagcg gctgcgcgtc acggttccag cgctgcttct gctgggctgc gcgctcgtgc
    12301 tgtatcagtt tctcggtggc aagggactgt ggataaacgc cggactgctc gcctgcgtcg
    12361 gatttttcct gtttggcccc gactcgctgc tttcgggaac catgtcacaa gacatcggcg
    12421 gacgcgccgc caccggacga gtcgccggca tcatcaacgg catgggctcc gtcggcgcaa
    12481 tcttcagccc gctcatcgtc gcccgcgtca gtgaaacatt cggctggagc gcgctgtttt
    12541 tcggtttctg cggcgtcacg atcgtcgcag ccgctctgct gctggtttcg gcgctgcttg
    12601 tcgaacagca gcaacgccac tgaccccgcc aaaagcaagc gcgaccaccc aaagggggtt
    12661 gtgtggccat accaccttgg tgcactcttg cgccaatcca cctacaaata tcgaggactt
    12721 ttcatgaagc gaagaagtta cagcttggtg ccgacccttg tcggggcggt gcttttcgtc
    12781 acgtcaggtc tgactggctg cagcaccgag cagagcaagt gtggcagcgg cctttctgag
    12841 tgcaacggcg agtgcctcaa tgccaaaacc gatccgaaca actgcggcgc ttgtggcaac
    12901 tcctgtgcga gcccgctcgt ctgtagttcg ggcagctgca gcgacagctg cgcgggaacc
    12961 caggtcgcat gtggccgcgc ctgcgtcgag cttgcgtcgg atgctctcaa ctgtggcaag
    13021 tgcggcaaca agtgtgaaaa cggtgccact tgcaccgatg gcaagtgcac gggcggcaac
    13081 cagcccgaat gcaaagaccg cattccgttt gcgacgagcg gcagctacga cctcgatgcc
    13141 aaggtggtgc aggtttcggg caagctgacc aaaaacggcg tggtcgtccc cgacggttcc
    13201 taccgcggca atttgacttt tgaaagcaag accacgggca cccgctcgat gccgtcgttt
    13261 aaggacaccg gtgacgccac ctaccagggc agcgtgttcg ccggcaccta tgacgtgttt
    13321 tataacccgt cgggaaactg ctcgcagacc ggaccgcttc cctgccagcg cacgctgttg
    13381 cgcagtggcg tgaacttgaa cgtctcgggc tcgctcgatt tcgacgtcaa gaccgtccag
    13441 gtttcgggca agctgaccaa aaatggcgcc atcgttcccg acggttccta tcgcggcagc
    13501 ctggtgttcg aaagcaagca gggtggcaac cgttcgatgc cgtcgttcaa ggacaacggc
    13561 gaagccacgt atcagggcga aatcttcgct ggcacctacg atgttcgcta ccaaaacagc
    13621 ggctcgtgca gtgaccgaag cagcctgccc tgtcagtcga tcgtcattca ggaaggcgta
    13681 aacctcaccg tttcgggcgc gctggacttc aacgtcaaga cggtgaacct gtcgggacgg
    13741 ctgaccaaga acggcgcaat ggttcccgag ggtgccaacc gcggctacct gtcgttcgag
    13801 ctgaagcaag gttcggcgcg cacgatgacg tcgttcaaag acatgggcga agccacctac
    13861 cagggcgaag tcttcgtcgg aacgtatgat gtcgtctacc gcaacggcta ctactgctcg
    13921 gcgggcggcg tgctgccttg cggcaacctg cggctcaagt cgggtctggc cgtcaccgtg
    13981 tcgggcgcgc tcgacttcga tgtcaagacc gtccaggttt cgggcaaatt gaccaaaaat
    14041 ggagtagctg ttccgtcggg aacaaaccgt ggctacctgc gcttcgagca gtcggacggc
    14101 aactcgattt ccatgcaggc attcaaggac accggcgacg cgacgtacca aggggaagtc
    14161 ttcgccggca cctacgacgt ggtgtttgag ccgtcgtact actgcaatag ctcaagccca
    14221 ttgccctgcc agcgtctgcg cctgcgcaaa gcagttccgc tcacgacatc cgggtcgctc
    14281 gatgtcgacg gcaagaccgt caacgtgtcg ggcaaactga ccaaaaacag ccagagcgtt
    14341 cccgacggca caaaccgcgg ttcgctgagc tttgcccaca aagacggcgg cgttgtctcg
    14401 atgctgtcgt tcaaagacat gggcgatgcg acgtaccaag gcgagctgtg ggccggaaat
    14461 tacgacgtaa gctacctgcc cagctattac tgcggcacat cctcgccgct gccgtgtcag
    14521 tcggcccagc tccgcaccga tgtccagctg acggtgagcg gcgcgctcga cttcgacgcc
    14581 aagaccgtca acgtgtcggg caagctgacc aaaaatggcg ccgcgattgc cgacagcacc
    14641 taccgcggca gcctgacgtt cacggaaaag agcggcagct cgcgcacgat gccgtcgttc
    14701 aaagacaccg gcgaggccac ctaccaaggt gatctgttcg ccggctcgta cagcattttc
    14761 tacaacccca gctacaactg ctcgatgacc agcccgctgc cttgtcagac gacgctgctg
    14821 gtcggttgcc cagcggccaa gtagcgagcc aaaccaaaac cccaagagag aatttttatg
    14881 aagatcaaga acctcattct cggcacgctc tgtgcgctgc tcgccagctg taccgagcca
    14941 aatcccccgg cccccgaaga catgatgact ccgtgggacg gcaacaccga aggctgtatg
    15001 ctcgggcagt tccgctgtgg atcgggctgc gtcgatgtcc agacctcgat tgacaactgc
    15061 ggacgctgcg acaacaagtg cggcgatcgt cagcagtgcg taggcggcgc ttgcaaagac
    15121 caagtcggtg actgtcgcaa agacggcggc tgtagcaagg gttactactg cgatctcaac
    15181 tcgggtgcct gcaagcaggg ctgcggcgca aaccccgact gcggaatgaa cgaagcgtgc
    15241 gacatctcga cgcacaagtg cacctgtgat tcgaacagcc acgactgcgc cggcaagtgc
    15301 gttcgttccg acgacgtgaa gacctgcggc actcgctgcg atgcctgccc aacggacccg
    15361 aacggaatgg cgctgtgcta tttgggcatg tgcgacgtcc agtgcaaaag cggcttccgc
    15421 gagtgctcgg gcgttccggg ctgctcggag tgctgccgcg actacgactg tccgtttgga
    15481 tcgggcaaaa tctgcgcgtc gaacaagtgc gtcgtcgcca cccgctgcac caagaccagc
    15541 gagtgcggca tcgacgaagc ctgcaccacc ggcatctgca ccagcaccgc gcctggcggc
    15601 acctgcacaa ccaccgcgga ctgcccgaac ggtcagatgt gccagagcaa caagtgcgcc
    15661 gtggtttcgt gcccggcgga atcgcatggc tttgccaact cggcctgccc ggtgggcgct
    15721 ggctgccgca aagcacgctg cgtgaacctg attgatgcgt cctgctcaac ctggagcgac
    15781 tgcggcaacg actacacctg cgatggcgcg acgggcacct gcaagctcat caccggcagc
    15841 cgcgactgca ccaccaacta caactgcaca ggctacggct acggctacgc ttgcgtgaac
    15901 ggccactgcc agaacgtcgg cgcgtcgtcg tactgccagg ctgacgtcga gtgcggcttc
    15961 ggtgcgtact gcggacgaat caacaagacg ggccgcacaa actactgcac cggcatgtgc
    16021 tcgggcaccg gctatccgag ctgcacgacc ggctacagca acacctgctc gacggttcgc
    16081 tgcaaagcgt atggctcgtc ggccaagggc tgctgctcgc gcattcacaa cacctgcacc
    16141 tatgactcgg actgcaatgg ctacaagtgc gtaagcggtc gctgcgcatc gagcgcatcg
    16201 tgcaccagca cggcgtcgtg ccagtttgcc gaaaagtgcg agtcgaacgt ctgcaagttc
    16261 accccgagcc gcacctgcac tgccgacaac gactgtggca acggccaagt ctgcatgtcc
    16321 ggaaagtgtg gccccgagtt cgaagcgtcc ggctggaact aacgccccct cctcctgtaa
    16381 catgaggcca tcccggcctc atcgatcaaa gttctcctga agaacgccta cttcacgctg
    16441 accgcggagg aacatccgcc ggtctcgctc gtccgcatgg tgcgctcaag cgcgcagttt
    16501 gacagccttt ccgacgcgcg ggcacagcac gaaaaactga tcgaagcgct cgatcggcac
    16561 ggacgccaag ggcgcttcat gctgaccgat ctgcgcgaag cggtgccgcg caacgatccc
    16621 ggctgggaag ccctgatggc agagctgcga ccgcggatga cgcgcggcta tcggcgcatg
    16681 ggcgtgctga ccaagaccgc ggcgggcacc atccaggtca gtcggcactc acggcaagac
    16741 ggcgtagaaa tgctgttgtc ggataccgag gaacagatcc tcgcgttctt ccgcatcgcc
    16801 gccaaaatga cccgctagtc ctgcactgcg ccccaaccga ttcgactgcc cgaccacttg
    16861 gcaagcatcc caagcagctc agcgacccat cgctcgcttc atgtcgagct tggcgtcttt
    16921 ttctttctga tcttcgcgct tgtcgaactg ctttttgcct ttgccgagcc cgatgaggac
    16981 tttgatcttg ccgtctttca aatagacctc cagcggcagc agcgagtagc ccttttggct
    17041 ggtcgcctcg tcgagctttt tgatttcggc cttgtgcaaa agcagcttcc gctcgcgccg
    17101 cggctcatga ttgaagcggt tggcgaagct ccattcactg acttgcatgt cgaaaaggaa
    17161 cgcttcgccg tttttggctt gggcataggc ttcggtcagg ctggctttgc cggcgcgcag
    17221 cgacttgact tcggaaccga cgagcaccaa gcccgcttcc atcgtttcct cgatgaagta
    17281 gtcgtgccgg gcgcggctgt tgcgcaaaag tacctttctg ccatcgcctt ttggggaact
    17341 catcgctgcc tactatagtc accgcggcgt cacttcatcg cgcgcttttt gcggaccaaa
    17401 cagaaactcg tgcaccagcg actggaagtg gtggactccg ttttcgcgtt tcccacaaaa
    17461 acgaccgcga tcatacgaac gcgagccgag tcctttttgc accgcttcgc aaatctcgat
    17521 gtcttccttc tgcaccagat ccccaaactc gattcctttg cgaatcgctt cgcgggcgtc
    17581 gtttggactg tgtgcgattc cttccggcag cgtccgattc accatcgctc cgtccgtgag
    17641 catgtaccac tcaaacacca cccgcgtgcg cgacaccgaa agcggaatca ccgcattaca
    17701 ctgcaagttg tccggataga cattgagcat ccagccagga aacacccagt agtacagcgc
    17761 ctgcgcatca cctgtggcgt cggaatacag acgttccttc ccgctgcctg acgggtcatt
    17821 ccgtttccgc agcggcgcaa tttgggaaga gtaatagcgc gcggtctcga cgcggtaatt
    17881 ggcatagtcc agttcgcgat acagcgacgg atgaacgact gggatgtgat acccttccag
    17941 gtagttgtcg atgtagacct tccagttgca cgcgacttcg tattcgacgc gctcgcacag
    18001 ccgaagccgc gccagtttaa atcgctccgt tgcttgggga attccgtcga tcatctctgc
    18061 cagcggcatg ggcgatgcag ccaagttcac aaacagaagc ggaccaaaga catcgaccgc
    18121 gatgggacgt agtccaaagt cctctttgcg gaagcatggc acatcatcaa attccggggc
    18181 actgcgcagc gctccggact gcgagtacgt ccagccgtga taccgacaag tgaaggtctg
    18241 ccttttgcca cagagagcgg ccaccggacc ggcgcgatgg cggcagacat tgtaaaacgc
    18301 gcgcagaatt ccgtcgctgt cacgcaaaac gacaatcggt tcacccgcta cgtctgcggt
    18361 gaaataggaa cccggtgtct cgacttgctc cagtcgtccc accggctgcc aggtacaaga
    18421 aaaaatccgc tctcgttcca gctcgaacac cgcagggtct gtgtaaagcc acgaagggag
    18481 tgtttcggca gagtcaacat tcgacgaaaa cggaggtatc tcgatcgtca tcttcccacc
    18541 acttttggct acagagcata gcaagcttgc ccaacccttt cgccacgcaa ttgtccgctt
    18601 tctttgcaga aagtgcgggc ggaaaaacca aggtgacaga aactctgtcc tgtggtgttt
    18661 cagtttggcg aacacaaaca ccgcaaggag agctccgatg cagaccgttt ggaagacgct
    18721 gtggacactg gccgcgtctg ctctattttc cgcttcatgt acgccggaat ttccgacggt
    18781 gacaggaacc gtctctgtcg aaccgctgca ttccaagctg gtaagcgatg actatctttt
    18841 gcgggtccgc ctgccccctt catatgaaac cgatccgacg cgacgctatc cgctcatcgt
    18901 gcagcttgat ccaacgtttg tcggacttca tgagtttgac atcacctgcg gacttgtttc
    18961 tgaacatgcc ggcgaaggtc gctggcccga agcaatcgtc gtcggtccgg attatcccga
    19021 tcccaacacc cggctgcgcg actacgtact tcccaaaccg cttacagagt cgttttccgg
    19081 acagaatgtc gatctgtttt atcgcgtgat gaaagaagaa atcctgccgt atgttgaagc
    19141 caagtaccgg atcgattcga cgaagcggat tctgatcgga cactcggcgg gagcgacggc
    19201 agtgtactat ttcacattcc ggcatgcgcc cccggaagct ccgctttttt cgggagccat
    19261 cgccgcggac gatggaattg aggagggatt tttcaccctg gaacgctggc acgcgatgcg
    19321 cacagactct cttcccatca agctgtttgc aacgagagca ctgtggaacg gagccgttca
    19381 ggaagttccc taccgggcgc tggtttcccg gattcagtcg cgcaattacc aaggtctgtc
    19441 gtttgcggaa aagacgcttg ataccgacca tgggggtgcg atttatccgt cgtttgaaca
    19501 gggtctggac ttcatctttg ggggcggaaa atgaactggg caaaagtcat tccttccatc
    19561 gcaagtggct tgcttgtcag cgcgctttcc gcaacgagca tcgctgaaga accgagcttg
    19621 ggaatcccaa gtggcggcgg cgagcacggg catccggtcc acgtccgttt ggacagcgcc
    19681 gcaacatacg gaatcggcgg ccagagctat ctgggaatcg atctgaaaat gagcggctac
    19741 ctgccggtct gggacacccg acttggcacg ggcagcatcg atgccggtgt ggtgctcgcg
    19801 tatggaaatg aaccaacgtg gctcgcgccg tggatcgata caagtcaggt gcacggagcc
    19861 acccaccgca tcagcttgct cgcacgccta ggacacactt ttcacatcgg ggtccgtcgc
    19921 cgcgccgcgc tcggtttcca cctgtttggc ggtcttcatg agtggatcag cgcctattct
    19981 gtaaactacc aaagcgaagg aatcagcggc agcgcggccc tcaaacgcag tctgtttgtc
    20041 tctggaggcg agctggactt tgcgtaccgt ttttccgatc cggtcggtct gtatctggaa
    20101 ctgagcgcac cgtttcccta ccagtcttcc tacgcacaga cgatgtttca cgtcggagcg
    20161 ggcctgactt tttatcttcg ctaggcgctt tggtggtttc gggtatcatc ctgcgccatg
    20221 cgccaagtct ggattacccg ccccggtccg cccgaagttc tgcaagtcaa agaatcaccc
    20281 gatcccgtcg ctgcggaagg tcaagtgcgc gtctctgtcc gcgctgccgg cataaacttt
    20341 gccgatctgg tggcacgcac cggaatgtac caagacgcgc ctcccattcc ctgtgtgatt
    20401 ggttatgaag tatctggtgt catcgaccaa ctggggcagg gagtccaggg attttccgtc
    20461 ggagaccgcg tgctcgcgat gcccaaattt ggcggctaca gtgacaccgt ggtcctgccc
    20521 gcaaatcagg tcatccggat gcccgacaag atgtcgtttg aagaaggggc ggcgctgccg
    20581 gtggcctatt tgactgcgca tcacatgatg ctgttcgtcg gcaatctgcg caaaggaatg
    20641 cgcgtcctga ttcactctgc cgcaggtggg gtgggtctgg cggcgctgga tatcggtcgt
    20701 gcgcacggct gcgaaatctt tggtgcggca tctgggacaa agcatgcatt cctaaaagag
    20761 cgcggctgtc agcatccgat cgattccgga aaagacattc cgtcgcaggt gcgcgcgatc
    20821 ttgggaaagg aatcgggtct tgatctggtg ctggacccga ttggagggaa gtccttttcg
    20881 gattcctaca acctgctttc cgagaccgga cgcttggtct gtttcggtgt ctcttcgatg
    20941 agtccaggca agagccgcag cttcctaagt gcgctgggaa tgctgatagg aacccgtcgg
    21001 tttagcccgc tttcgctgat gatggacaat cggtcggtgc agggcgtcaa catggggcat
    21061 ctgttttcgc ggctggacct gcttttgccg cagtttgaat cgcttgtcgc gatgtatgaa
    21121 cgcggcgaaa ttcatcccta tgtcgatcgc acgttccgct ttgaggaagc cgcccaagcg
    21181 catcattatc tgcacgatcg caaagccaaa ggaaaagtcc tcctggtccc ctaagacatc
    21241 ccttttctga cggagaactg ccgtgtttgt gcctcctcac cacccctact ttgatgatcg
    21301 cgcggcgatg ctttggcatc gcagctttga tgaagcgttt gcgcaggcca agcagagcgg
    21361 acgacggctg ctgattgtcg tcacccatga ggaatgcgga ggcagtcggg cgctgctcga
    21421 acgggtgttt cccaaagagg aagtcacaca cgagctgcgc agtggctttg tctgtctggc
    21481 cgccgatctg catcggcacg aaccacaggt ggcggagctt ttggcccagg cgccgcgacg
    21541 cgagcccacg cccgtctgtc tctatgtcgc ggcagaggaa aaaggtccgc ggcttctgct
    21601 ttccacggct gggggacgtc cgcccgcggt ctttgtccgt gatctgaccg atgcacacgc
    21661 gcgaaagtaa cgatcaaaac gccaagcgcg gacgctattc ttcgtcgggc agctcttcgt
    21721 aaaaacgcac aaacaggttg ttgtcgctgc cgccgagccg cagctcattg agcggacacg
    21781 gcgagctggc catgcagcgc gcgtgcagtg ctttgagctt tctgcggtcg tgcgcaaacg
    21841 cattccagtc gccccacttg gggtccacaa tgtcgatcag ccagtcggtc tgcggccccg
    21901 gatcattgag cgacgcgccc tgcccaccaa tttccgcgcg gccactcacc atgtaggcca
    21961 ggtcggtttc cgacagcaca ccttgctcta gctcgctgcc gggatgggtg tggtacgtcc
    22021 cgatcagctg ctcgcccggc gacagtggcc ccagatcggc ggcgataaaa tcaagcgtgt
    22081 ccccggaaaa acgaaacggc ggcaccacca ctcgcgtgac atccacgtcg tcgccgagcc
    22141 gcttgccgag cagaaacgcc gccacttctg cgacttcgtt tttggcaagt ccctcttgtc
    22201 ccgtcgacac ttctttgagc cggcgaagct gacagacctg ccgccccgaa aaacgtaccc
    22261 gcgacagtcg tccgtcgagc atcacgacca agagccgcgc tcgcgcctga cagtcactgc
    22321 ccgtcgacag gcgaggatgt ggcacctcat cgagcagcgc gctcaagaca gccgcattgg
    22381 cgaacgcatc ctggggatcg agccgcggaa tcagatcgcc gataagccca cgctcgcgcg
    22441 cccgctcgag tgcatgcaca ggtggaatcg cgggacgctt cttgtgcaca agcccaaaca
    22501 ggaccgcgcc cagaccaaat gcatacagcg cacacgcaaa cagcgcgcgt ccccactgcg
    22561 tcgctcgatc ccccgaaaga acccgtgtca tcgcgtggct gccgtcgcca gatcggccaa
    22621 acggtagcct ttagtatcgc ggacctcggc ttataaatgc acaaattttt cgtaatctac
    22681 aaaacttaca attgcttgta gccgcagtgc ttatggcggg attggcaggg gccggggcgc
    22741 aaagggagtc gtcgcagccg actgggtatc aaggtcggct tcgggatctg gatgaagcga
    22801 caccgacagc accgaatcag cgctggcacg gaccgcaagg tcgtaaaaac gggtcaggta
    22861 tccgacggcg cgtacttcca atcgatgcgg ccccgccggc atccgcaccc gcgctggctg
    22921 gccacccgtt tggcgaagca aaagcggctg ttcatcgagg aacaccgtcg cggtcgcagg
    22981 tgaaacgcca agcaccaaag ttccaagtgg tggcgcagcg ggagcggcat ggcaaccgca
    23041 caaaccgacg acaaacggca ggcacaggcg cacggccctt tgtttgcgga cagaaaaaga
    23101 ccgggtcatg gtgccgccgc ctccgcttct gggacttctg ccgatggcaa gtcttgcgcc
    23161 ggcacacagt cgagccgcag cgccgatgga ccgagcggca ccacctgcat ccgcagcccc
    23221 gccaccggcg ttcgataaag tgccgtgccc agcgtttcgg tgctggtctg ggtcataagc
    23281 tgaaagtgga cttgttcatc gccaccgtcg gaaaaaacga tccgccgcgg ctcgacccgt
    23341 gaaacacccg gcagtgccaa gagcgctttc cacagcttgt tcatcaccgc agccgttcgc
    23401 agacccgtcg ccaaaagcag cacaccgccc cctggtcgcc caagcagtcc ggtccgttcc
    23461 gccagcttgg tcagcaaggg tcgacccact tggcccaccg cttgatcaag cgccgcatcg
    23521 tcccgctcgg caaacgccac tccatcgccc gcttcctgct gcggcggcgc cccgggagcg
    23581 acaacaaact gccaaacccc gcgcacatgg gccgaccaaa gccgcggcgt tgtaccgcgc
    23641 acttccgcca ccgccgattc caggttcccc tcgaagctga gccgcatgcc cggccaagtg
    23701 ctacccggcc cacagcgatc ggcattgccg agttctacat tcagcgagcg ctggcccaaa
    23761 agctcccgcg cagaagcctg tagcttttct atcagcggtt ccgccatggc gagcttgacg
    23821 cagagcaaaa cgctcggctt tccgtcgctg tgcttgggtt ttggcagtgc cgtttgcagc
    23881 tcacgcaaga gccgcgcgac atcgacctgc gagacaattt tgaacagaag ccgcccgtct
    23941 tgctcggtct cctcgacgac gcgataggac gatacaaact cacgcgcgcg cggcgcaacc
    24001 agatacagtc gaccacgcag ctcgggcgcg ctctgctcga ccgcctgcaa gagcgcctgg
    24061 cccatcgcgt cgctgagcgc ctgattccgc gtcttgctgg catcgtccgt aagcgccacc
    24121 tgcaccgccg tctcgacttg cagttcctgt gcgcacgccg accgcaggcc accgccgagc
    24181 aggatcgcaa atagcgccaa tccaagcacg agccgcgcgt gtcgaaagca aacttgcatc
    24241 ttagtgttcc tcggacggac tcgccccggc atcaggagcc ccagccgtcg cgctgtcgct
    24301 tgtagcacgc tctttgctgg ccgcaagcgg aagcgcaagc caaagcacga ccgaacccgt
    24361 cgcaccataa tcaatccgct gcaccgtcgc ggtctttggg tccagcttgg caacctgatc
    24421 ggcgccaccg aatcgcgaaa gctcggcctt gtgctggcga accagaagcg gcagcgcttt
    24481 gcggagcttg tcttccgccc gactgcgcgc cactcgctcc gctttcagac gcgcaacatc
    24541 tgccgacgga ggatacaaat cggcggcaca gcttgcgacg gccatcagcc ggccttgggc
    24601 aaaatctacc tgcacgcccg ccgccaccgt ctgtggcttt tccgcgtggg ccgcgacggc
    24661 tgcggacaca agcagcgcac tgccaaaaaa ccaagacctc aaagcgctcc tccctttttg
    24721 tcaaaccggc ccggcgtaca aatccacgcc gcaatgtcat cgaaaagagg ccgcagcgtc
    24781 gaacttcgca cgatgtccat gtgatccgga tcgatcggat cgccaaaccg tcgcccaacc
    24841 tgccagtgcg tgatcggtcc gctggtttcg gaaacaaagc gaagctgaca gcccggtatc
    24901 gccatcagcc ggtcccccgc gccagcgacg ctaagaatcg gttccttcac ccgcggaagc
    24961 tgggccaaaa acgaatgccc atggcgatcc gcccagtcgc ccgaaaacac ccaactgccc
    25021 atctgcgcaa ggtacgcata cggttcgtcg tcggacccaa tcccaagccg caaagccggg
    25081 agatagccaa gcggtcgcgc cgcccaaagc agcgccgaat acgacagttt tttggccagc
    25141 cagcgaaagc gccaagactc gtgccgacgc agccagacat tcgccgccaa cagcaccagg
    25201 ccatcaaacc gcaccgcacc ggccacgtcg aacgagcgcg cctgccacgc cagcgccagg
    25261 tgcccaaata gactgtgtcc aacagcgact cgcggcacat ccggcgccac ttgctgtagg
    25321 tatgccgcaa gcgccgccac atcaaacaac agcgtgtcgt agtcccagcg cgcgcccaaa
    25381 gacggccctg gctggctctg tccgtgtccg cgctgatcaa tccacaacac ccacagcccg
    25441 gcagccaaaa gcgccgacaa aagcccctgc cctgccggcc tatcgagcgt tcgccgatcg
    25501 accatcatcg cgtgggaaag cagcaccacg gcccgcggcg cctcgttgcc ccgaaaccac
    25561 tccccacaga tgatcacacc gtccgacatc cgcagcgcca ccggatcccc accgcgcaat
    25621 gcggcatgtg acgcggccac gacttcaaag tcgtggggga cgggtcgcgg tttcgcgtta
    25681 ctcgacgttt gggccacgat tcctcgtttt gtcggcccat ggtagcctga atcgccgggg
    25741 gtttgactgg ccatggccaa gctcttctat cgggatgtct cggggacgac aggatcaatt
    25801 gaactgggat cggtgccggt gctcatcgga cgagcgacgg actgccagat ccagacacag
    25861 gatgcgcagg tatcacggcg gcacgcgcgc gtgatcttcg acggctccgg ctattttgtc
    25921 gaagacacgg gcagcgccaa cggtgtgttc gtcaacaacg aacgggtgcc gcgctatcag
    25981 ctccgccacg ggatgacgtt tcgctgcgga catctggaag cgcgcttcga atcggaagcc
    26041 gccgctgcac cagcggcccc cgtcgcggct gtacctccac caccgcccgt catttctgcg
    26101 ccgccgccac caccaccggc tgcggttgcg tcaccaccgc caccgtcgcc accaccgccc
    26161 gctgcggctg cgccgccacc accgcccgcg gtgcccgcac aggcagccga gcttgccagt
    26221 ctgcgcagcg aactggaaag cgagcgacgc aagcgcaccg atctggaatt cgaaatcacg
    26281 gagcttaagc gcaagcttga cgaaacccag gcacggcccg ccgccaccag cgacagcgac
    26341 agtgaaaaac tgcgtcgtcg ggtcgaacag ctcgaatcag agctcaagcg caaaggcagc
    26401 gcggcagctc cgacgggtgg aggcgcaccc agcgaagcac aaaaggctgc cgaggcagag
    26461 cgcgatcgcc tgcgcacccg cgtcgctgag ctggaagcca agagcaaaga gccggtcaaa
    26521 aacgacgatc aagaaatgga actgattcgg ctccgtcgca aagtcgagca gcttgaagcc
    26581 gatctgagac gaattcgcgg cggcaaaccg gcggagccag cgcccgcacc cgcaccaaca
    26641 ggcgatgcac tggtcgccga gctggaagaa aaagtccgac ggctgatcag cgagcgcgac
    26701 gaagcactgc aaaaagccag cgcgccagtc ctcgtcgcag cggcagcccc accaccgacc
    26761 gatcccaaaa tcgctgagga gctggaaaaa gccaagcgtc ggatcgatca actggaatcg
    26821 gaacttcgtc gaaagcccgt cggacaagtc gccgacaccc agcgcgcgac cgagcagcgg
    26881 accgagctgg aatcggcgct gcgccagctt cgcgatgttg aaaaagaacg cgacagcctg
    26941 cgcgagctgg tggcgcgatc gtcgggaagc ggacgaccgg ccaaagcgac gctcgatgga
    27001 attcagaccg tcagcgacgg actggccgac attcgcgccg cgctgcgggc agccggtgac
    27061 gatgtggcgc ttgaacaact ggaacagctc cgcacgacgc tgcgtcaggt gtgtagcgcc
    27121 ctcgggttga cgacgtagcc aaccgcagac acgacggatt tatttgttgc gaccgggctt
    27181 ttcaggttcg gcgcgcagaa tcgtaaactc gacttcggcg ggctggattt cgtaggcaat
    27241 tcccggaggc aaattgacga tctggggccg cttgtggaac accgatccca ccggcttgcg
    27301 ctcgtcgggc gatgtatcca gcataagctg cggcacgtcg aggaagcgat cgagcccctg
    27361 accacgcaaa atcaccatcg ccgtcgtcgg attgagccgg acattttgcg gctgaccatt
    27421 tggcgtaatc ggcacgctgg catagtggcg ctcgaccagc tcgacgacga ttgtggcggt
    27481 aaacttcggc ggtggctcgt cggccagtcg cacatgcttg ggcagcggcg ctagctcgac
    27541 gggaactgtg acggtatcgc gcacattcac aagcgacagc ggacgagaca gcacgttgtg
    27601 catgctttcg agcacgttct gcgcaccgcg caaccgcaca agccgtggcg tcggtgtcgc
    27661 ctgtttgaag cgataacctt cgggcggctc gccttcgatc gtcaggttga ccccgagcga
    27721 aaccagcgct tcgggttcaa agtgcatgta catcccgacg ggattgaagc tttcgattcg
    27781 cagccccggc ggcagacgca ccatttcggg cgaaaaacgc agctctccgt cgctcagatt
    27841 cgacatgtcg atcacgatcg gctgcagcgc ggtgtcttcg atgcggctga tccgcgccca
    27901 cggtccccgc accgtcaccc gcacctcgct gagcttttgg tccgacacca tgatgcggcc
    27961 ccgcggctcg atgtaattga cgcggacgta cagcgtcgtg ctctgctcaa gctcggtgcg
    28021 cacaaagatc gccagaccga cggcgacgat cagcgcaaac gctttgagca agaggtcttc
    28081 gacgaagact cggtagagga agtcgcgcac gggggctact ccgtcttcgc tttggcaccg
    28141 tctttgtcgg gtgccggttg ggctgccagg tcgtcgggac gaatcgccgg cagcttgtgg
    28201 gtgtcgccat ccagctcgac gggctttttg gccaccgcgc cgcgatcggc gggaatcggc
    28261 agcgtcgcca gttcatgaac ggacggagcc acgcccgcag gagccaccgg ggtcgacgga
    28321 accggctcga caggcagcgg ctgggtcggc attgcgtcat tcgacggacg cgagcccatc
    28381 gacggcagcg gcacaccgcc caggctgatg tcattggccg agcgatcggc tttgccggtg
    28441 cgcccggtcg gacggtcttt gcttttgttt ttcttttttt ggaacagccc gagcagcgct
    28501 ttgcgcacgt tcagcgcgtc gagatcccga atgatgttgc cgctaaaaca gaccgaaatg
    28561 gtgccgcgtt cttcgcttac gacgacgaca actgcatcgg tttcctcggt cacaccgacg
    28621 gcggcccggt ggcgtgtccc cagttctttg tcgagcttgc tgttgcccga cagcggcagc
    28681 acggcgcccg cttgcgcgac tcgcaggttg cggatgatca ccgcgccgtc gtgcaggtga
    28741 ttggcaggct cgggaacaaa cagcgtcacc agcagttctt tggtgacttt ggcatcgagg
    28801 tcttgcccga cttcacccag gtgatcggtc agatcggcat cgcgctccag cacaatcagc
    28861 gcaccgattt tttgccgcgc cagctcgccc gcggcctgga cgacttcttc gatgacgtag
    28921 gtttcttcgt actgccgtcg cataaacagg ttctgaccga cctgcatcag cgcccgacgg
    28981 atgtcctgct gaaacagcac gacgaagaaa atgatgaagt agtttatcag gttttcgaac
    29041 agccacgaca ccgcgaccag atcgagccgc ttggcaagaa agtaaagccc ggtgataagc
    29101 gccaggccgg tcattgcctg cgccgcgcgc gtgccccgca ccagcttcag cgctcggtac
    29161 aggatgtaaa aaatgatgat gacgtcggca caagcgatca gggcttcacg ccagttgatc
    29221 gccgccgcga gcgtccgcag agacagccac atcggggcga aaacgctcat gagccgggcc
    29281 tcccccgcga cagtgccgca tgcgtcacga gcgcgagctt cgtttcggcg acatcatgaa
    29341 cccgcacgat agaagccccc gcttgcgccg ccaccacggc cccaatcagc gacggaatca
    29401 gcctggaatc gaccggctgt ccagtcagcg taccccacag cgacttgcgc gaacaaccga
    29461 ccaaaagtgg acgctcaagc tgcgctttca gacgctctgt gctgcgcaaa agcgtgaggc
    29521 tctgctctgc ggtcttggca aaaccgattc cggggtcgta aatgatgcgc tgcaaaagcg
    29581 atgggctgtc gtcggtcagc atgcgcgctg cgagcgaaag ctctgcacaa acagtgtcgc
    29641 acagatcgcc gtcgtagtcg gtaagctcgc gcatgtttgc tggtgtgcct cgggaatgca
    29701 tgaccacaat cgcatcgaaa ccggccagca gccgctgtct ttgcaagtgc gaaagccgcg
    29761 ccgcacctgg cgagtcgagc tcgggcgaaa acaggtgctg tggaaacgcc agattgagca
    29821 cgcgaaagcc caagtccaag agccgcgccg cgacctcggc gtgtcgggta tcaatcgaca
    29881 gcaccgccgg cagaccgagc cgcggcagct ccgacaccac gggaaagagc cgctgccatt
    29941 cttcctctgc ggacagaagc tgggccagag gtcgcgtcga ttccgcgccc aagtcgatca
    30001 gctgggcccc ctgcgccacc agggtttcga catgacgcag cgcggtccgg ggatcaatca
    30061 gcttgccacc gtcggaaaac gagtctggcg tcaggtttac gatccccatc accgcacagg
    30121 tttttccatc aagatccagc catttcccgt cgctgcgttg caaacgtaga ggtgcaatct
    30181 cgggccgcag aaagacggga ttttggtggt tcgcgatcat ggtgtgtgca gcgtgtctaa
    30241 gcttggacga ccggcgcgcc gcgaccttac agcgacgcag tggaaaaaat gcgtggaaat
    30301 actagcctcg ccgccaagca aaaggaaaat tgcggcgcgc catgaaccgt tctgtgagtg
    30361 ccaagtacat cgatccgctc gatcacatct ggatctctgc cgccaaagag ctgggactga
    30421 aagtacaaag gtcaccgcac ggctacgcgc acaccgatgg caaaggcaac ctgtatttcg
    30481 cgcctccgga tgaactggac gacgacgact gcctggctca gatgattttc cacgagctgt
    30541 gtcacgcgct ggttcaaggc gaaggcagct tcgacaagcc tgactggggt ctttccaacg
    30601 atgagcaagc cgatgtgtac gcaggcgatg tgacgcgcga gtgggcgtgt ctgcgtgtcc
    30661 aagcttcgct gctgcgtccg tatggactgc gccgcctgct cgccccgacg acggattttc
    30721 gtgcatttta tgacaagctg ccccgtcacc cgctcgccga agcccccgag cgcgaagcag
    30781 cgctggccca agcgggtctt tcgctcgcgc atcggcctcc gtttcgtcgg gtgctaagcg
    30841 atgcactgat cgccacctgt gaactgcacc gcgcggtcag caaatccgcc cggcagccga
    30901 cgaatcccaa agccgaaccg catgaagcgc cgctgccgat tttgtggcac agtgtcccgt
    30961 cgcccaagct gcacaaaagc ggtctgccga tgtcggacgg gatgtttgaa gcaccgcgcg
    31021 gcacgacctg ccaggactgc gcgtactgtc tgccgcccga aaagcgcggc gcgtcgcatc
    31081 gctgccagcg cactgcggtg gacgatacgc caggcaaagt ggtgaaagaa acgacgggag
    31141 cgtgcgcgct gtttgaaccg ccgctcgact gtcagcgctg cgccgcgtgc tgtcgtcatg
    31201 ccttcgatct ggtgccggtg cgaaccaccg atgagatcac ctggaaacat cccaagctgg
    31261 tgggcaaagc cggcaaagac ttgaccgtgc tgcgtgatcc tgtccgtcgc tgttgcgcgg
    31321 cactggatgg ttctttttcc gatggctatg gctgtctgat ttattccgaa cgcccgcgca
    31381 cctgtcgtga ttttacaagc ggcactttca actgcttgga agcgcgacgt cgcgtcggtc
    31441 ttgatgcgta aaccccattc ccaagcggac tcccaaagat gacgtacacc agtttttcgc
    31501 tttcggtcag cgatcacgtt gcgcagcttg tgctgtgtcg tcccgacaag ctcaacagcc
    31561 tgacgatcga attttttcag gaactgctgg atgtctgtcg tgcgctggaa agacgcagcg
    31621 atgtccgcgt actggtgatt tcatcgacgg gaaaacactt tacgtgcgga ctcgatctga
    31681 tggcgctggg atcgctcgct gcggatctgg gacatggcga gcgatcacgc gcggcgtata
    31741 ccttgcgaac ctgcgtgcac gaacttcagc agagcatcag cgcagtggaa gcgctgcgaa
    31801 ttccagttct gattgcggtg catggcggct gcgttggtgg tggtgtggat ctggcgactg
    31861 cgtgtgatct gcgctactgc acggcggatt cctttttctg cgtgcaggaa accaacgtcg
    31921 gtctggttgc tgatttggga acgcttcagc ggcttcccaa gctgattccc gatggtatcg
    31981 cgcgcgagct ctgtttcacc ggacgaaggc tgcccgccga tcgagcgctc gcgctgggac
    32041 ttgtcagtgc ggtgtttccg gatcacgaaa cgatgctgcg cgaagtgaac aaaaccgcgc
    32101 aagagatcgc gagcaagtca ccgcttgtga ccgccggaat caaagatgtc ctacactttg
    32161 gacgcgacca cggaatcaag gatggtctgt catatgttgc ggtttggaat gcgggcatgc
    32221 tgtcggccca agacctgatg gaacaggccg cggccaaagg ggccggacgt cccgcaaggt
    32281 acgacgatct gccggctccc aaagacctgg tgaaagtccg ttaaaggact ttgaggacaa
    32341 gctgcgcgag cagtccaagc agcgtgatct gtcccagcgc aaaacacacc gttcgccatg
    32401 gctgcttctg cgcaagatac atgatggtgt gaaacaggcg cgccgcaaaa tacagtccaa
    32461 aatacgccca tccgcccgtc gcagaaccac cagtcagcgt aaacagcagc gccacaaaca
    32521 gaaacgccgg aatgttttcc agatcgtttt ggtgggcgcg cttggcacga agcacatccg
    32581 gcgcatcttg cgcttccaca tgcgatccgg ggttgactcc ggtgtcttcc ggattcacca
    32641 ccacgccggc cttgcgtcgt gcatttgccg ttgcaaacgc cacgcccacc atcttgagaa
    32701 ccagcagaac agaaaacacg atcaggggtt tggctgccgg aagcgcaaga aatgccgtca
    32761 tcaaggactc ctttgctttg gggggcgcac gggacgtgtc cgcagcgtgc gaacttgtgt
    32821 tctatatcac agtggcagca gcgccgatac agatccacat cgcgccatgc cacttgcatt
    32881 tgcggccaag gtgcgtcaca aacttaacat catgaaaaaa atatctgctc tgtttctggg
    32941 actactgggt actgcttctt tgggtgctgt gctgtgcgga tgttcgcaag gcggcagccc
    33001 cccttcttcc accgatacgg gcaattcgga tctgtctgtg atgaattcca ccgacggtgg
    33061 cggtaccgat gacatggcgg tggcaccgct tgatttggcg atgcccgatc tgtcgatgcc
    33121 ggacttggcg attcccaatg atccgcacat tccggcaagc tgcacgggcg cacagctcaa
    33181 cctcgcgagc aaatttccga tgggcgcatc gtatgtggtg ctcgcaaact acacgatgaa
    33241 atatgagcag cgatcgtgta cggactttac cggctgtggc agctggggac catcgacacc
    33301 gtcgtgggga ccgagcggaa gcggcaaggt ctacctaaaa gtcacgggca ccggaatcat
    33361 cgttgcagtg gtcgataaca gcgcaggatc agcctacgga acgccggtct acaacttggg
    33421 aatggactgt gcgctcagcg cgggagtctg gaactgcgga cgctatcacg acaccgaaat
    33481 cgtcgagggc gcgaacttcc tgaagtttga aggcaaagac aagttcagcg gcgttgagct
    33541 tccggtgttc ggtgaagtgc tggcaaactg cgcgcgcttt tacggcaccg tacaaggcgt
    33601 caagaccgcc ggttcctaca aggaataccg cggcggcttc ctgctccgtt actaatcagt
    33661 ttgggagtcc ggcctggggc aaagacagcc gaaggacgcg catggggact ccgcctttgg
    33721 gaccaaacgt cgcgccccgc atcaccggct ttatctcttt gtcgttggca agcgccagct
    33781 ccgcgtgcga aaggatttcg tgcgcgacca gcttcatttc gtagtttgca aacgccgcgc
    33841 ccaaacagcg acgggttccg ccaccgaacg gaacgaactc gaacggagtg aacttgtgct
    33901 ctaggaatcg ctcgggacag aatcgctgcg ggtttggata gatcgccgga ttgtgatgaa
    33961 cgccgatcgc gctgaccgaa accgcatgtc ccgccggcac tgtgtagggc ccaagctcga
    34021 tgggaacgcg cagtttccgg aaggtttccg ccaccacggg atacagccgc agcgattcgt
    34081 ttgcacaggc atccagatag cgacactgcg caatggactc cgcttcagga tgtggtccga
    34141 gcgcatgcag ctcttgccgc aacttcacca gcgtttcggg gttccggtac aggtgataaa
    34201 acatccacga taggccaagc gccgtggttt cgtgtccggc gagcagcagc gtaaacagct
    34261 catcctgaat ctgcgcatcg ctcatcgtgc tgccatcgtc gtagtgcgcg tggatcatca
    34321 tgctcaggat gtcttcgctg ggtgggcggc tgcgactttc cgcaatccgc gccgccagtg
    34381 cgttttccat ttcccggcgc gtttcgtagg tcttggcaaa cagcttgaag ccaaaatact
    34441 ctcgccgcag cgctggaaaa aacagcatca gcggactcag actctccatg tacgtcaccg
    34501 ccagcttctg cgtgcgtgcc gactcttctg gatcggccag cccgaacacc gcattggcga
    34561 tcaccgacag cgagatccgc tgcatcgcgc cctgaaaatc aacgacttcg ccttctcgcc
    34621 atgagcgcat ttcctgctgc acggcttttt gcatcacctg tccatacgcg cgcatccgag
    34681 cgccatggaa aggcggaccc aatagcttgc gctcgcgccg atgtttttcg ttggtgataa
    34741 ccagtatcga attcggcccc gtcaccacgt cgatgccatc ttcgggataa attccgatct
    34801 gcgaagtatc gagtgtgaag atggtcttta cgtgttcggg attgcgggtc agcacaacgg
    34861 ggccgtttgg tccatggaac aaaaaggtgt cgccgtgctg tccgaaggct cgctcataaa
    34921 aacgatacgg actgctcagg tactgcagcg cgttatacgc tgcgatgcgg gaactttcgg
    34981 gaagcggctg ggatgtcatc gcagactcct tttctgacat cgtagcacca tcccaccctg
    35041 cgcggtgggt cttgtcgacc agcctgcggc tgtgcggttt gatggccgcc tagaaagcac
    35101 gttcctatct gatgattccg gggatttgga agcgagcaaa gcaggggaag ggaaaggaac
    35161 tggcagaagg gaaccgcgag gaccgtgcaa tatgaagcat tttgtctgct tcatattgca
    35221 cgctgatcag aagttacgcc gcgtttgcgc cgtggcactt cttgtacttc ttgcccgaac
    35281 cgcaggggca cggctcgttg cgtccgacct tggcttcttc gcgcttggcc gtcttctgct
    35341 tcggatctgc gccttcgact tcggcttgat cgtcgcggct tatgtcgcgg gtgctctttt
    35401 cgtcttccgc gcctgatgtc gccgatgact ggtgctgaag cacaacacga cgcgccttgt
    35461 gcttaaactc cggcagcttc tgttcggctt cctgctcggg cgtttcgggc tgcgcttgct
    35521 gatcaagacg gaccaaaaac agcttcttgg ctacgttctg cgagatggtg tccgtcatct
    35581 gcatgaacat gttgtagccc tcttttttgt actcctgttt cgggtctttc tgaccgtagc
    35641 cacggagtcc aatgccttcg cgcagatgat ccatcgcctt caggtgatcg atccactgcg
    35701 agtcgatttc ctcgagccag aagtgccgcg caaagtacag gaacgccagc cccagctcgg
    35761 cctcacgcga ttcggtgtac ttttcgatct gttcccacgc catgtcggcg atcgaaccga
    35821 cttccaggtt tacgtccgtc agcgtgaccg caacgccgaa ctggtctttg atcgccgctg
    35881 caagcgcggt catgtcccag tcatcgggcg tcttgtcttc tgggcagtac tcgacgacga
    35941 ttttttcgac caagtcgtat gccagatcga acacccgctt gcgctgctga accagcgaca
    36001 ccgcggtttc gtgaacgatc ttctttactg ccttgtcttt gtcggtctgt tcctcgctgc
    36061 caaactcgat caccgatcca aagcggcgat acagctcgtg ggtcagatca cgcgcactgc
    36121 gcagcttgcc tgacttgccg ctcgctttgg cgatctcgcc gggtttgtca ccaaagtgcg
    36181 atccagcctc tgccgcttcg ccttcgggat tttcccagaa tgcgtcgacg atgactttga
    36241 ccactggttc cagccgcttg tcccagcctt cgtattcggc tttgacctta tcgacggacg
    36301 gtgccgtcac cgcaacatgc tcgtcgctgt cggcggaatc cttcggcgca ggcaccggga
    36361 ttcctttgcc gcccaggatc gcttcgacat caaagcccgg cagccgacct tccagcaccg
    36421 accgacgcag cgcatagatg ctcttgcgct gctggttcat cacgtcgtcg tactcgagca
    36481 ggtgtttgcg catgtcgaag tggtgcgctt cgactttgcg ctgcgcgttt tcgatcgcgc
    36541 ggttgaccat cgagtgttcg atgggtacgt cttcttccat accgagccgt tccatcagcc
    36601 cggtgatccg ctcggcaccg aagatgcgca gcagatcgtc ttccagcgac agataaaacc
    36661 gcgacgaacc gggatcgccc tgacgaccgg cgcgaccacg cagctggttg tcgatgcggc
    36721 gcgattcgtg tcgttccgta ccgacgatgt gcaggccgcc aagctcgcgc accttctttt
    36781 tgtcttcgtc ggtggtcttt ttgtgctttg caaacgcctc tttgtattcg ggcgctgttt
    36841 cgtcgcaggc accgggacgg caggccatct cttcgccgta aatttcggtg cgcgccataa
    36901 actcggggtt gccaccgagc aggatgtccg taccacgacc ggccatgttg gtggcgatcg
    36961 tgacctgctt gctggcaccg gcctgggcga cgatggcggc ttcccgcgcg tgcgctttgg
    37021 cgttgaggac gttgtgcgga atgccttttt tcttgagcat tgccgacaga acttcgctct
    37081 tttcaacgct gaccgtaccg acgagaaccg gctggccacg ctcgtaacac tcggcgactt
    37141 cctcgatcac ggcgcggaac ttgccacgct cgtttttgta aaccaggtcg gggtgatcct
    37201 tgcgcaccat cgacaggttc gtcgggatca tcaccacgtc gagcttgtaa atcttgtgaa
    37261 actcttccgc ttcggtgtcg gcggtgccgg tcatgccggc cagcttcttg tacagacgga
    37321 acaggttctg aaacgaaatc gtcgccagcg tctgcgtctc ttcttcgatc tggacgtttt
    37381 ccttcgcctc gatcgcttga tgcagaccgt cagaccagcg ccgacccggc atcagacgcc
    37441 cggtaaactc gtcgatgatc acgactttgc cttcgtcggt gaccatgtag tttacgtctt
    37501 ttttgtacag cgtatgggcg cgcagggcct gctggacgtg gtggttccac tcgatgtttt
    37561 gcgcgtcgta caggttgtgg atgccgagca gcttttcgac tttttcgacg cccgaatcgg
    37621 tcaacaccgc cgagtgatgc ttttcgtcga cggtgtagtc gatgtctttc ttaagccgcg
    37681 gaacgatctg attgaccttg tagtaaagct gcgccgactg ctccgccggg cccgaaatga
    37741 tgagcggcgt gcgcgcttcg tcgatcagaa tcgagtccac ttcgtcgacg atcgcaaagt
    37801 gcagatcgcg ctgcacgtag cgctcgatcg actccttcat gttgtcgcgc aggtagtcga
//
LOCUS       DAORGN010000020        37419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_397_length_37419_cov_5.731613, whole genome shotgun sequence.
ACCESSION   DAORGN010000020 DAORGN010000000
VERSION     DAORGN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 37419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37419
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_37419_cov_5.731613"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..471
                     /locus_tag="PKL17_04025"
     CDS             130..471
                     /locus_tag="PKL17_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNK43926.1"
                     /translation="MAVPTSAADLFDTILPPKIAASPDKAREINAIYQFIITGEGGGQ
                     WNVDLASNPPSITQGEKPGASCTIEVANTDFLGMLKNPALGMQLFMTGKLKVKGDPML
                     AMKLQKLFSLG"
     gene            520..1698
                     /locus_tag="PKL17_04030"
                     /pseudo
     CDS             520..1698
                     /locus_tag="PKL17_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617603.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
     gene            1725..2315
                     /gene="pdxH"
                     /locus_tag="PKL17_04035"
     CDS             1725..2315
                     /gene="pdxH"
                     /locus_tag="PKL17_04035"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNK43927.1"
                     /translation="MVISSHSDPLTLFAHYFSRAKEDVSHDPTAVALATATPDGFPSL
                     RMVLLKAIDDRGLVFFTNYESRKGRELTENPHAAMCFYWPRSEVQVRAEGVVERLSPS
                     DSDAYFQTRPRLSQVGAWASQQSARLPKRQDLLDRVAEVESQYAGKDIPRPSNWGGFL
                     LVPQRMEFWQGEPGRLHDRLVYERNDSGWQTFRLFP"
     gene            complement(2354..2545)
                     /locus_tag="PKL17_04040"
     CDS             complement(2354..2545)
                     /locus_tag="PKL17_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNK43928.1"
                     /translation="MSKTSPQTGHVHGPDCNHDHDHGDLDPSVFLAKQPFIRQDPKVG
                     RNDPCPCGSGKKYKKCHGS"
     gene            2669..3901
                     /locus_tag="PKL17_04045"
     CDS             2669..3901
                     /locus_tag="PKL17_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNK43929.1"
                     /translation="MSAAPQGRLSERGRNAPASPIRKLEPLARRASDRGVKIYHLNIG
                     QPDIATPTEFVEHARLTPGEVLSYSPSAGLYPYRQALAKYYSSLGLAVPIEERDLLIT
                     TGGSEAILFTLLALCDPGDEVITPEPYYPNYGMFALMAGAELKTLPTRIEDGFRLPSP
                     TEFARAVTPKTRAILLCNPSNPTGAVYSREALAEVVKLCREHNLTLICDEVYRDFVYG
                     GQKKQSVLELPEAADVAVVIDSLSKRFSACGARIGCLLSKRADILDATTRFAMSRLSP
                     PTLGQLGGMAAIEDPGPFVDGMIEQFRRRRDVLTEELAKLPGVLAPQAQGAFYLMTKL
                     PVDSAERFCSWLLDDFSHNGETVMLAPGPGFYKTPGAGQSEVRIAYVLGEQPLRRAVD
                     LLGRALVAYPGRTEAQKP"
     gene            3898..4821
                     /locus_tag="PKL17_04050"
     CDS             3898..4821
                     /locus_tag="PKL17_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43930.1"
                     /translation="MTEAVQSLQALLGRISSFFDIFDLSFIVSGATSLAALCLYYKLH
                     APGGFPEWLASVYGGILFVLGCYVLGMLSFILGRLLRRIVMRVRGPFLRRQARQISHA
                     LREHNIWVKDDDRSMLALCISTLLATPAQDTASKPSPAKGKKSGAASDESAKKQTDES
                     ETKRLEQTAHRLYTLLWTQVRQQHDLSPSHVLLNRYWVMAALCDGMVVASLLWLGVVV
                     CSWSRLSAMQLGFMGNLGIALSAIAVVLFVREAERYTAVQIDDLISTLAWDRSRPPSP
                     PAQVQTAPSSPNGAASVPSPAAASLSGQPTQ"
     gene            complement(4737..6482)
                     /gene="hflX"
                     /locus_tag="PKL17_04055"
     CDS             complement(4737..6482)
                     /gene="hflX"
                     /locus_tag="PKL17_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNK43931.1"
                     /translation="MQQLHGNLTGLSASDKQLLERIYRRRVAPSEVVSEELGAFLGQC
                     STTVKRQVGVLIDRGGIVTHVIVGDHQKLFLPDLGRARAGRGRLRGLRLVHTHLHGEP
                     LSRDDLLDLAKLRLDLVAAIGMGRGGRSERLFVAHLLPPNERGELWRELPPVPLQRQE
                     VVVDELIPALEDEYQKAAQAAAMVDDGRDRAVVVHVVYTGPDERSRGEVHNPVARVAE
                     LRELCRTAGLQVLDVITQKRAQLDPRTLLGKGKLDEVVLRALQYDATVLIFDPELTPA
                     QARTISDATTLKVIDRTMLILDIFAQHAKSRDGKMQVELAQLKYTLPRLIEKNTMMSR
                     LTGGIGGRGPGETKLEINRRRARERITQLQRQIDQLSQERHQRRKMRRRRALPVIGIV
                     GYTNAGKSTLLNTLTRSEVLVENKLFATLDPTSRRMRFPDERELIVTDTVGFIRDLPQ
                     DLVTAFRATLEELSEADLLLHVLDASDPAMEEQRRSVEKILFELELSDKPKLFVYNKI
                     DQLDAEGFASLPHDREAVALSAIEPETVRPLLDAIDQALLLSPLRRYWVGWPDSDAAA
                     GDGTEAAPFGEEGAV"
     gene            complement(6501..7427)
                     /gene="dapB"
                     /locus_tag="PKL17_04060"
     CDS             complement(6501..7427)
                     /gene="dapB"
                     /locus_tag="PKL17_04060"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNK43932.1"
                     /translation="MSSGTFPSERTNPDESSDEFQPSSATAGKGGSELHMVIPIRIGM
                     YGAFGRMGQIIMRTLLSGFEPDRDPKLMLTAAVEATNHPHLGRDIGPSCGLGPVGLDL
                     TDDLARALSNVDVMIDFSTPGATALLAAAAAETNTPLVIGTTSLHAAALASIDAAANR
                     VPIVCSANMSPGVNVLISLLKRAAAALPDYDAEIVELHHKWKRDAPSGTALLLADSIE
                     GARGRKSDLRLGRSGMSGPREAGELGIMAIRGGDVIGEHTAYFFGTGERLELTHRAQS
                     RDVFARGALQAARWVVGQPPGIYGMEDVLGMY"
     gene            complement(7424..8332)
                     /gene="dapA"
                     /locus_tag="PKL17_04065"
     CDS             complement(7424..8332)
                     /gene="dapA"
                     /locus_tag="PKL17_04065"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNK43933.1"
                     /translation="MAQASAARFSGVFTALVTPMHKGSGPVPEIDEAALVRLVEAQVR
                     GGVQGLVACGTTGEAAAMTADEQAKVIRIVVDAAAGRVPVLAGVGSSSTATSLSLAKT
                     ALSLGVTGLLAVTPYYNKPPQEGLYQHFLALCSLGAPIVLYNVPGRTGCDLLPETVAR
                     LCDRPEIVALKEASGQVGRTQALSRLLGDRLLLLSGEDALNLPLYSVGASGAISVLSN
                     VVPQAVVDVFRRFGRGEHAAALELHQRYVPLVDLLFSEPNPIPCKAALHMMGQLDNVL
                     RLPLVPMSQPGQARLRLELTALGLLP"
     gene            complement(8387..10024)
                     /locus_tag="PKL17_04070"
     CDS             complement(8387..10024)
                     /locus_tag="PKL17_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNK43934.1"
                     /translation="MLETLSKGFRLAREKLTGQAELTEENIDAALRDVRMSLLEADVA
                     LPVVRSFLSRVKDKAIGQTVKLRAKAELPTDGKLGEKSLKSMEVTPEWHFIKICQEEL
                     TGLMGNPEGGPTEFNWAKKGPTVIMMVGLQGSGKTTTVGKLARWLHKIHKRKPLLVAA
                     DVYRPAAVQQLQVIGEKLGMSVFTGDGKNPVEICDEAYKYAYTKGYEVVLLDTAGRLA
                     IDEPLMRELDQIKSRVKPQEVFLVVDSMIGQDAVNTARTFHDRVSTTGFILTKLDGDA
                     RGGAALTIREVTGQPIRFLGVGESLDKIEEFRPEGLASRILGMGDIVGLMKDFEQVVD
                     TQKAEQDAMRMLKGKFDMQDFLEQISVIQQLGSLKDILAKMPMFGLQLPEGANIDDGE
                     LVKIKAMISSMTMVERRQPELFIETSWEQVISGGQVKGRRRVANYVESRVRRIARGSG
                     RKEVEVMELLHKFALMRQMMLSMGAQAGLLGKIPGLRSLSKLKQMAGMDLGALMGAAG
                     MPQVEGRRSAPRPNVDKKREKVKRKAAKEARKRNKKK"
     gene            10191..11186
                     /locus_tag="PKL17_04075"
     CDS             10191..11186
                     /locus_tag="PKL17_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNK43935.1"
                     /translation="MESSDLGLLLALDALLQETSVTRAARRIGLSTPAMSHALGRLRQ
                     RLSDPLLVRAGRQMVLSPRAESLRPQVAALVSQARRVMEPEQPFLPQLLRRRFTVVLS
                     DYVLAVLGPTWDRLLSAEAPHLELRSIPNSPDDATLLREGSADLAIGIYGVLPPELRT
                     RPLLTDRFVCAVRKDHPQIGKRLQLDEFIAIPQVQIAPRGKPGGYVDDMLAERGLSRR
                     VARAVPYFHSALFLVSQSNYLVTVSERIATMYADSLGLRILPPPLPLRPYALSLLWHP
                     RFDADPAHLWLREKLLGAAKLVASDTHAHARTRLDPSDPNSGQTRKRPGRRPQKS"
     gene            11388..12134
                     /locus_tag="PKL17_04080"
     CDS             11388..12134
                     /locus_tag="PKL17_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNK43936.1"
                     /translation="MTANQNVTRLGRRKRILFIAANASVSPQTGWPIGFWWAELTHPY
                     WAFTEAGYEVEIRSPDGGSLVADSFSDPEDSSGYSADDILSLGFKKSPKHAALLQNTQ
                     SISDVRVGDYDGVFICGGQSPMVTFRGNQKLQALVAAFYEAGKITALVCHGTCLLLET
                     RLSNGQLLVEGKTWTGFANSEEEYADSFVGRRIQPFWIESEARQIPGSNFVVDQRFRD
                     FAVRDGLLVTGQQQFSGAAAARLVIEALGR"
     gene            complement(12137..13693)
                     /locus_tag="PKL17_04085"
     CDS             complement(12137..13693)
                     /locus_tag="PKL17_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNK43937.1"
                     /translation="MLGPKTRRWLVAAPLCAVLVPWPIAHAESPATPSADKTASHATD
                     GTPAFDDASGARKASAHSQFGSSGSPPPASATASGFDFGSYGRVGIGSDLRGHSGHGV
                     NVVSFGSRLEKPAYLELNFYYGGTIGDDPAKRWRVVFVPGYSGDLFHATGNFSQNFAI
                     RNAYAEVENLGVDGLSLWAGSRMYRGDDVYLFDFWPLDNLNTVGGGAAYALNQKRTLL
                     QIHLGMNRLADFYQFQQIQVPARRLPGSTTILSQQSATTTVTTLDRQRLVVSAKATQF
                     LRNLSDLPNAKFILYGEFHYLPKGERAPELTGLPPQPLPDDYGYVIGAQVGGWLRSFV
                     FANLFFRQAGGLGAFGDLGKPFGFGSDVRVFGAKESTLALSANYESQAIGVMAGGYFR
                     RFSDASQAEDSGFSEGAVALRPTIYFTKYIHTAVELSYQVRRPTTVDQDSGRYLKPMV
                     ARASVIPIVAPLGRGTYSRPFLYGIYTLSWLSSDAQYYLFEPQDVRAGHSLVHYLGLG
                     VEWWFNSSYR"
     gene            13957..14979
                     /locus_tag="PKL17_04090"
     CDS             13957..14979
                     /locus_tag="PKL17_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43938.1"
                     /translation="MMMTKRVLNVAMGLLLSTAACGVTADGTAGGVADGVIDESLRTT
                     DVPAEEADLTGTANSTFYRVTHPDLRRCVYPLCGGYFTERVNQKTATCSDGTAQPECR
                     ILEFDYAALKLDATSQAKLTSAVQNGQALLRGTIGKTAPIAGKTYDKLVVSEAWVARA
                     LSTPSGTYARSKELPLACAGCPTFLHANLNKTTSQRSHTVLFDATKFSSTLTAELQKA
                     MQSLPEGVLTAGTLTTSGSRQVWNVSEAYTRFVASGPRLGKLGESCGSRGLPFDCETG
                     LFCQRPDTAICGRADAPGTCQKKPEACTAVYMPVCGCDGKTYSNRCVAYSYGVSVDYD
                     GACKTP"
     gene            complement(15010..16278)
                     /gene="coaBC"
                     /locus_tag="PKL17_04095"
     CDS             complement(15010..16278)
                     /gene="coaBC"
                     /locus_tag="PKL17_04095"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNK43939.1"
                     /translation="MDATSDPSLPLHNRNVVLGISGGIAAYKAAELTRLLRKAGASVR
                     VIMTHAAQQFVTPLLLQTLSGNAVATELFDRTQESEIGHINLADSADLLVIAPATADV
                     IAKLSLGLATDLLSTVALACQAPLVICPAMNVNMWNKPQVQHHIAQLRSFGAHIVGPA
                     AGELACGWVGQGRMTEPSDIVLSLAQIVQESGPRDLVGKHIVVTAGPTYEAIDPVRFI
                     GNRSSGKMGFALAESAARRGAQVTLVAGPVALETPRGVTRKNVESAREMAQAVLPLVE
                     QNPPPDCIVMAAAVADHRPSEVSPHKRKKQDLGTSPTIALVENPDILRALGVAKQGIA
                     PLLVGFAAETQDLDAYARRKLREKGCDVLIANNVAEAGSGFGSDTNRVTILVRRGDDI
                     DVVPLPLMPKQEVAQHIWNQLLPLLPRPGS"
     gene            16421..18049
                     /locus_tag="PKL17_04100"
     CDS             16421..18049
                     /locus_tag="PKL17_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNK43940.1"
                     /translation="MSSALPATSSARHRLWLRVLGILLFWICSVRSAAALPDETWLVV
                     VGNNRGDASEVELLYGERDAKELAEVLRTQGNIASDRVRILLDEDAETVRRTLVGINT
                     QLRSQTAKGNPSALLVFYSGHADADALHLRGTRLPIDELRGLVSSSPATMRILIVDAC
                     RSGAVTRVKGIKSAQEFVIKMEDRVEAEGTAMISSSTAGESSQESDRLRASFFSHHLI
                     NALRGAADKNGDGKVTLSEAYAYSYAQTLRSSGQTLQLQHPTYAYDVKGSGDLVLTTI
                     QSAQHNVGRLRLSSASTYLITEEKETGAIVAELSTARDHAVVALPRGKYFVQRRGSHE
                     YREYLASLLPAQEIDLETIPYRSVRYDQLVRKRGGSRASVHGAHLLLGMRGSILPGET
                     VTPHLLLGYAADLPWLSVGTRLRGSTTSYQAIDGGYTARHHELGLSVLLQRYVDLPWC
                     SLAFGVQMEGTWYAQRYSQTLRMISARDTLGIGFSALFAVERQLLHGIGIRIEGGPMA
                     NLFRHVESQFGEATDTVTRSVFTFWLAGGATWRF"
     gene            18085..19023
                     /locus_tag="PKL17_04105"
     CDS             18085..19023
                     /locus_tag="PKL17_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43941.1"
                     /translation="MIRGMICGIIGATLWGCGDGLTDADYRGTPLWRMSGGLEQSSFD
                     TSVYPTVRIALFWNPSGETPRPASQLVEQTSASQAVAVGNPYILNMFDLPGADHIART
                     YDGRSRGFGIGRVLGYADLDANGRYSDGDKFVSLWSDVALFYAASDLPSWASPTSGAL
                     SAGFYQLLLPQRCDKPIPTPTDSGTCGITLGKKCGTDADCGTGGTCLRETNIPWPTGY
                     CAVANTPSASCRPAAAAYYGIPQDIPNPPTARGYYLKPCQSDQDCVTQTQRDAGVYRC
                     DLGLFACTPSVGGKMSVGGIRDIEAELTRQPFCMTN"
     gene            complement(19129..19305)
                     /locus_tag="PKL17_04110"
     CDS             complement(19129..19305)
                     /locus_tag="PKL17_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43942.1"
                     /translation="MNDRIAQRLASLVAEYEAGQRVLVELEAKQKQVQQTMLRIEGAI
                     EVLKELQINPRGDQ"
     gene            19539..20198
                     /locus_tag="PKL17_04115"
     CDS             19539..20198
                     /locus_tag="PKL17_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43943.1"
                     /translation="MSSKIIDDLTHLPNIIAGLGLGIAQAQSKMDLDYLQSLERLVVM
                     AKSLLGGQKASSAGNVPTTEEDQRKLEQFQGVVKDFLTVLAPTRYQFTETTLTVKLEL
                     SQHLDVSGSAGLSAGIGAVAINASLAVGYGSDYRGAAECKTVLHAVPTDANTMRTLLD
                     RANQLGTKELSMPQRTQVDSAVQNQASSVFEKMVGYAPATVKDSATASATAATGSENR
                     G"
     gene            20250..21377
                     /locus_tag="PKL17_04120"
     CDS             20250..21377
                     /locus_tag="PKL17_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNK43944.1"
                     /translation="MPDDPDGPNSHPNDRNPPFAEQRILVLHNADFGEQPDGDESYES
                     RADVANQAHDVARALVTRGHFVDVVGIDRDELPSLMERLRTDPPDLVFNLVESLAMQD
                     ENCVAATAILELFGIPFTGSGTLAMSLSCDKQRSKHLLRGDGIPTPRAAVVEPQFRSR
                     ISDLNAVAAVGYPAIVKLLSEHGSQGLSFDSVVYDEESLIRQLRVMRDYFPGRRILVE
                     QYIDGRELNVGLLGDRLLPVCEIDLSAIPTGKPRIVTYTGKWRPESEEYRMMPSSKVA
                     VGLSAALTERIQQVAQRSFSALGVTDYGRVDIRLSQDGIPYVLEVNPNCDISDGAGLS
                     RAALAAGIPYDALIEQVAYSARQRFSGGITPPTEKKRATGS"
     gene            complement(21403..23178)
                     /locus_tag="PKL17_04125"
     CDS             complement(21403..23178)
                     /locus_tag="PKL17_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43945.1"
                     /translation="MTSRQTFRRRWLVCAALAIAPLLSACGRADGLSSQVCSAGGVCW
                     QRPTPQGNTLRAISGISSKFIWAVGDYGTILHYDGKSWNKSPSGTTLDLHGVYCVGAK
                     DAFAVGDEGLVLHFDGSDWNRIDSGTKETLLSTWASGAGDVWMVGRRGSVLHFDGKSI
                     KKASASVSDYSTVFSVYGFDSSDVWISGSSGLVRHFDGSDWKTVSSGAYNDIRAVWGH
                     RAREVWLASGSTWSGRLSRSQNGGSGESFGPSLASGMDALWGYSATDAWAGSATGRIY
                     HFDGNEWKVQKDSLAIHALWGSSPNDIWAVGPSGLIAHYGGKEWKKETDPTTSPVSAM
                     WAKGPSEVWAVQGSELLRFRDGGWKAEKVPGYGFAQTRTLLGFSGSDVWAFGTGTTVR
                     HFDGKKWQDTLLAGRGASSLTAAWASSARDIWAVGQSGTVVHYTGQEWAVLSKPTTEN
                     LTAMDGSSARDIWAVGDNGESIHYDGEQWGSITTGETDRLTSVASCGARDVWAVGVKG
                     VITHWDGARWGRVKAPDDINLWRVLCRSAKEAWAVGDRGTVLRFDGQSWSKVPSGTNQ
                     NLTSLIYDGRRLWIGGQHGAIFVLR"
     gene            23289..24116
                     /locus_tag="PKL17_04130"
     CDS             23289..24116
                     /locus_tag="PKL17_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK43946.1"
                     /translation="MRRVGQDLIDILKWVGFAPWSLFWMTSAITYGIIFRNDPHTPLS
                     WARRFWSPGAAVISGCQLTRHPGFVPEPGKPYIFMMNHQSMFDIVCAFIHVPVNLRFI
                     AKKVLQSIPFLGWYMTVTGMIFVDRRKRDQALKSLEAACERIRSGIPILVYPEGTRSK
                     DGSILPFKKGPFMFALQAGVPIVPVAINGSRGILQPGSPFVRAGHIDIVMGEPIPTAG
                     LDKQDRDALMRRVRDAIIDLQVSIGGPGGDKERYLAAEGKEGVADPTIDSIGPDSSV"
     gene            complement(24041..28369)
                     /locus_tag="PKL17_04135"
     CDS             complement(24041..28369)
                     /locus_tag="PKL17_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNK43947.1"
                     /translation="MPQQSERTRLFQAVARVEVSTHLGEQRGTAFLVAAQHALTALHV
                     VADRHADSPVALGSVRLHFPSQTIAAELVAFDRQADWALLRLIEVPQDVAGQPIAPLL
                     LLPLSEDDLSPGPLSWLSVGFPEARPDGLWVSGQVRSTLSQVGQSPAIQLFSDEAAAG
                     RGAPLAGLSGAPVWVDGAVAGLLRWATLDDAGNSVAGSVFACPIDRVFAALAERGIGK
                     AEPRCPYPGLVSFAADQGRLFFGRRSEIDWLTEHVRRDRLLLVIGASGSGKTSLLQAG
                     LVPTLGPAFVVRLVRPGQAKLPSLDSVQSLLATAPAQRLVLIIDQLEELFLSSPRSEQ
                     QDFFAKTAALSALPSVALVLVLRADFFPELMASRLWPIDPSQRLEVAPLSGDRLVEAI
                     VEPAAQQGLRVEPALVQRLLAEVADEPGALPLLQETLVLLWERQKGRKLTLRAYDDLA
                     QLVDPSLPRAAQVSGLGAALTLHAEQVWAELSVGEQRLCRRTLLRLTQFGEGRPHTRR
                     QQPWTALVSTSDDRSQTEALIERLVRARLVTKSAGPDAGGDGKNNQSESPQVVLVDLA
                     HEVMLRAWPRLRRWLSELRQAERVRRRLEQQADDWQRLASRGGGLLDAVETKEAEQFL
                     QSPDAQELGVSETIRALVSESRRHVEQTEQAARDAAIEKARQKLLFEQEREKGRARLW
                     KTVATMTLLLAGSATALSFWALRERRWARVREEEAKHWAESEAEARKLATDKQRLATL
                     RFLTAQAQVRQDSAPDLAVLLGVAAFTLQGGAEAEGLLLQLLQRRAPLVRMIHTDGVP
                     LRSLAQSSDGNRLAAGFDDGTLWLLDAKSGRLLHKPWRAHTGTTSALAFSSDGKRLFS
                     AGEDKLVRAWNTESQDSLGQAVGGPLLGHGTAVRSLSASPDGRYLASGSDDGMILLWD
                     QKTGELVGPPLREHQRAVMALTYSPDSRYLLSGSDDQTVRLWDAKAARRLHVLLGAQK
                     TVRAVAFVGKLAAAGDWDGHLLLWDVQTGKPQGKPIDGHGLSLTGLVADPSRQSLVSA
                     SWDGDLRKWQPETGQQIGRPLHPRQGALQALVLHPSLRSVWTGNEEGRLLEVELDERS
                     PLERQSHVLSSELSSLASDREGKWLAVGGWDKQITLFRVDAGGLQPDKVLRGHDGSIS
                     AVAFSEDGTKLASAGFDKTVRLWDVATGTAKTLSTGGDLTSALVFSVDGTRLYSAGFD
                     FAVRSFDAKSGRPAEQTLQGHSGAIWGMGLSSDGKRLCTASQDQTVRIWELGSGRTQT
                     MLQGHSRAVTDCRFLPDGEHLLSSSEDKTLRLWDLATGKTQGEPLRGHVDRITGIAVS
                     RDGLRAVSASEDQTLRLWDLTTLQSIGPGLRGHVRSISAVGLSPNSQRAYSVSHDGTL
                     RVWDLAVSSWLALACRLAGRDLNREERERFLGDVKLPPLCQTDESGPIESMVGSATPS
                     LPSAAK"
     gene            complement(28369..29907)
                     /locus_tag="PKL17_04140"
     CDS             complement(28369..29907)
                     /locus_tag="PKL17_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43948.1"
                     /translation="MSDLHLGGAQAAAGERGFQICTQTAALAELIDRLAARNPDNGGE
                     ELVIAGDFVDFLAESDGTGENGDPVWKALREDDAVAEQLLHTVAMRSPDVFAALRRFS
                     ARGHTLTILLGNHDIELCYPRVRQALKHLLGVPSHVGLRFIYDGEAYAVGDALIEHGN
                     RYDGWNAIDYDALRRVCSLKSRGQAIPPSLRFPAPAGSQLVAEVMNPVKRSHPFIDLL
                     KPENTAALPLLLALEPGARSQIAKLAKLAISAGELRLSKPALPTRSGDISAHVPNVRG
                     PQPDGELGFRTPMSDGEAELLAMLGESLGEAEVSPFLSQIGGAPSTPRDMAFVTRGGE
                     RGDIAARPRGTDPAPGRDSKWSGGLSWAKLLLTGGGAPESRLKALHKALRAFCDPSLF
                     AIDKSPKGEPLFEAAKELARISPFRYIVMGHTHLARNLPLDGGGRYLNSGTWADLMRV
                     PDEVFADDPQVSAAALQQLVADMADRRYDRLIVRRPTYVRLELAGDQVVEASVETYQS
                     GAPL"
     gene            30202..>31678
                     /locus_tag="PKL17_04145"
     CDS             30202..>31678
                     /locus_tag="PKL17_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK43949.1"
                     /translation="MANTGQRREAFGAYYFVVEIEGERFAHFRSASGLKSEGEVVPLM
                     EGGWNAAEHKLVGRTKYPNIILKQGSAGPEMWRKRQRFISDTTGDLRRFDGAIIQMGP
                     GGRAVATWTFTNGWVCKWEAPDWDAGKNEIAIETIEIAHEGLALNGSGPTPPRSKHSS
                     GAKSAASSSAAKQAAAAAKSGAGAAAKGAAATAKNAAKGATDAAKNAAKGAADAAKTA
                     AKSATDAAKDAAKSAEKAAGAAANKAVADATKDVAKAAGKAGVDNFKPEVTVGKDGIH
                     AHVEGDFKKNVAGVPVSGHVSADAVANKSGVKATASGSAKAEAGPVKAEAKADAGVSV
                     GKDGVKGHAQGEVSAKADVKAGPVDVHADASAKGRVDEKGATGSVDANVTAKTQAKVG
                     PMDVKAEAGAKVHADEKGSHAEAGVKAQAEGKVGPANVKAAADAGVSADSKEGVKAHA
                     GAEAHADAKVGNTTVSGDAKAEVHADKTGVHSSADAHVGAKT"
     assembly_gap    31679..31778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31779..32732)
                     /locus_tag="PKL17_04150"
     CDS             complement(<31779..32732)
                     /locus_tag="PKL17_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43950.1"
                     /translation="MSTDLFAASFGGCVAIGACMDALFVDARVGVDVFVDRHRTDFFG
                     GTRVEIAICARSTRSDGRTRRSIGRPSHWTLLAFGVCLRIDTGLNAFLIGNDTGFCVA
                     AEGSVADFAVRMSPDFGMSFGPVRARRSARARISIPVDWTLLAFGVCLGFDAGLNAFL
                     IGNDTGFCVAAEGSVADFAVRMSPDFGMSFGPVRARRSARARISIPVDWTLLAFCMGL
                     CFDAGGGALFIGMNPSTGFRRDGGATDLLFGLGPDVRIRRAVDARLVGMCFRFGVAAH
                     GRISDLGIGVRPDFGMSLRAIRTGRSARARISIPVDWTLLAF"
     gene            complement(32783..34327)
                     /locus_tag="PKL17_04155"
     CDS             complement(32783..34327)
                     /locus_tag="PKL17_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK43951.1"
                     /translation="MCPLTLASPSLAIAQPSQSSAPTSATKTLTIDDALKIGQSQNRD
                     LAAARARLKGAHADVERAMAALLPTLNAQARLTVNEPEVSVPFDQRQQVFSSLLQGAA
                     ISDASVALAKTGMLQSGSLTNALKDYCNSATGANRQAVDGLCQQIINPAAQLGKTPPS
                     VADIDSTLTSANPQIVIQPRLQLDATIIANVPLLVPAAYPALQGAKLGFRAQERQLDV
                     TTAQVLQSVATAFFAAAGAEELVAARSNTIAVAQKTVDNAKVRLQAGVVNKVEVTRAE
                     LALIQAKQRLLESQDSRASAYRMLATMLKLQPGSFQVTPPPEPSTELGAETDLVDQAL
                     KGRPELLSLDLSMQAASSQVTSSWLRYSPNLSLFGNVRFTNATGFSGRVASLAVGAQL
                     DWQIFDGFVRDAQRHQFDAQKRDAELRIEQLRDSISDEVINARRTVVTKRQGLVTAQR
                     ALQMAQETLNLVRLQYEAGTATQLDLLTSQDQMDMAAVGLAQARFDLSLAVIQLRRLI
                     GAPLGG"
     gene            complement(34397..34798)
                     /locus_tag="PKL17_04160"
     CDS             complement(34397..34798)
                     /locus_tag="PKL17_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HNK43952.1"
                     /translation="MLPQKKDVMLELLKDAWVYVVLDPRKEGVILPDHLRSQPRLVLQ
                     YGYNMPMPIHDLTIDDEGIKATLSFQRTPYATLIPWSAVFAMHDGEKRGLVWEEDVPK
                     DMDAQPPPPEPPPPPPKPGKKPRPSHLKLVP"
     gene            34901..35323
                     /locus_tag="PKL17_04165"
     CDS             34901..35323
                     /locus_tag="PKL17_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43953.1"
                     /translation="MTHATTHEKDETHWLYRLTADEWLRAAATELRHSRQAFENKSQR
                     AAVAHARRAGGMAINALLWLRSDDSYGRSYMDHLGRLAKDPAVPSSLQVDAQALLAMP
                     MQAALVQLGPKGDPKQAEPAARILEWAQKLAQSHQTGN"
     gene            complement(35337..36122)
                     /locus_tag="PKL17_04170"
     CDS             complement(35337..36122)
                     /locus_tag="PKL17_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNK43954.1"
                     /translation="MSVVLSYEQAGRIARLTLSRPEAKNALRPADWQQLLLHVDDIAR
                     KPEVHVVCVSGSGGAFSSGGDLKTMPQRLAMPIAARREQLLRDSKVILALQQLDVPVV
                     AAIPGVCMGAGLSLALSCDLRLASESATFGAVFHRVGLSADFGLSWLLPQAVGTAKAM
                     HMLMTAAVLGAQDARACGLVHEVHPDAELEPAALALCEKLARGPKLAQAMTRRALGRA
                     ATSDLATMLEWEAHSQSLLSKTADAAEGVLAFSEKRPPNFRGE"
     gene            36216..37034
                     /locus_tag="PKL17_04175"
     CDS             36216..37034
                     /locus_tag="PKL17_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK43955.1"
                     /translation="MHNILLSALHLDFAALLDPVAQAASAAASKPGTYDSIRDWYKSA
                     DRITLLTLGAMILCSLAAVTVAIERTLSLWNIADSSRRLAEQVRRAVYRGALADARTA
                     CQRSKSPLADVMLVGFERIGRSSNEALFAAVERERQRMVLSLRGPLWVLGTIGATTPF
                     LGLFGTVVGIMGAFRKIGESNQAGIQVVGPYIAEALIATAVGIGIAVVALLVFNYFQS
                     RLARTSIELRLAIEEFLEELTEAHGRDPDAGNPKSDDKSDGKDKHAKTASEEAA"
     gene            37036..>37419
                     /locus_tag="PKL17_04180"
     CDS             37036..>37419
                     /locus_tag="PKL17_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK43956.1"
                     /translation="MAIGKVPGSAGEGDLDEEGIFAEINITPLTDIFLVLLIIFMVTS
                     SVIADAGARSGLRVNLPKGATKEVSPGSKDVTVAIGQDGKMMVDGKLVDGTELARLFA
                     EAKTRDPATQIIVHADESTAHGRVVS"
BASE COUNT         7341 a        11934 c        10995 g         7049 t
ORIGIN      
        1 cctagtacga acgcaaccga catttcaaca ccagaatcgg tgccgcattt gacagtcgtg
       61 cggcctgcgt gttacccgag atgaactgcc gtcaaggcgt gattcaccaa tcgaaggagc
      121 ctcaaaccca tggctgtgcc tacgtctgct gctgacctgt tcgatacgat cctcccgccg
      181 aagattgccg cgtcgcccga caaggctcgt gaaatcaacg cgatctacca attcatcatc
      241 accggcgagg gtggcggtca gtggaacgtc gacctcgcgt cgaatccacc gtcgatcacc
      301 cagggcgaaa agcccggcgc aagctgcacc atcgaagtcg ccaacaccga cttcctgggt
      361 atgctcaaaa acccggcgct cggaatgcag cttttcatga ccggcaagct gaaggtcaag
      421 ggcgacccga tgctggcgat gaagctccag aagctgttct cgctcggcta agcactgccc
      481 gtctccgcgt ttcccacccc caatcccgca cggaaaatca tgcagccgct ctctggcgtt
      541 cgcgtccttg atctgtcgcg gcttctgccc ggtccgtatt gcacgctggt gctgtcggac
      601 cttggggcgc aggtcgacaa agtggaagat cctcacgtcg gggactacct gcggctgatg
      661 ccaccgctgg gtccaccatc gggggaaaat ccgggtctat cgggtcgttt ttcggcgata
      721 aaccgcgaca agcgctcgct ctgcctcgac ctcaagcatc ccgagggtcg caaagcgctg
      781 ctgagactgc tcccccacta tcaagtgctc gtcgagagtt tccgtccggg ggtgatggcg
      841 cgacttgggc tttctctcga aacgctgcac gctgcaaatc cggcgctcgt cgtctgctcg
      901 atttcgggct acggccaaga cggtccgtac aaagagcgcg ccggtcacga cctaaactac
      961 gcggcgctgg cgggggtgct gtcgcagtcg ggtccgtcac cgcaagatcc gccgcatcca
     1021 cttccgattc agctggccga tctgggggct ggcgggctgt gggcggcggt gggaattttg
     1081 gcggcgctgc gcaaagccga gcagtgcggc gagggcagcc acctcgacat ctccatgtgc
     1141 gaggggacgc tgagtttttt gattcccgat ctgggcaacc tcgaagtgct gcccacagga
     1201 tcgccaccgc cccagcgtgg cgctgatttg ctgacgggtg gcgccgcttg ctacagcgtg
     1261 taccgcaccc gtgatgacaa gtttgttgcc gtcgcggcgc tggaaccaaa attttgggcc
     1321 aacttaaacc atgtgctcgg acggcccatc gatccaagcg agctgctggc cccccccgac
     1381 gagcaagagc aaattcgcag cgaactggca gcgatttttt tgcaaaagac acgcgccgaa
     1441 tgggaagcga tctttgcaaa tgccgatgcc tgcgtcgaac ctgtgctgtc gccaagcgaa
     1501 gtcaaagcgc atcctttgca caaggcccgc gcggtgtttt tcgatggcaa tgaatcaccc
     1561 gatggtccgc ccttgcccca ggtaaaaacg ccgctgcggc cacgcggaaa gccctgtgga
     1621 acaccgccgg aacatggaca gcacagccgc gacatcttgc gcgaagcggg cttttccgaa
     1681 ggtgacatcg acgcactcct caagctgagc gtgaccaaat agccatggtg atctcttcgc
     1741 actccgatcc gctgacgctg tttgcgcact atttttcgcg cgccaaggaa gacgtaagcc
     1801 acgacccgac ggcggtggct ttggcgacgg caacgcccga tggttttccg tcgctgcgca
     1861 tggtgctgct caaagcgatt gacgaccgag ggctggtgtt ttttacgaac tacgaatcgc
     1921 gaaaaggtcg tgagctgacg gaaaatccgc acgcggcgat gtgtttctat tggccgcgca
     1981 gcgaagtgca agtgcgagcc gaaggcgtcg ttgaacggct atcaccgtcg gattccgatg
     2041 cgtatttcca aacccgaccg cggctaagcc aggtcggtgc gtgggcttcg cagcagagtg
     2101 cgcggcttcc aaaacgccaa gatttgctcg atcgcgtcgc tgaagtcgaa tcgcaatatg
     2161 ccgggaaaga catcccgcgc ccgtcgaact ggggcggttt tctgctggtt ccgcagcgga
     2221 tggagttttg gcaaggcgag ccgggacggc ttcacgatcg attggtctac gagcggaatg
     2281 acagcggctg gcagacgttc cgcctgttcc cgtagcgaaa cggtctgacc gtcgattcag
     2341 cggttggggt cggttagctg ccgtggcact ttttgtattt tttgccgctg ccgcacgggc
     2401 atgggtcgtt gcgaccgact tttggatctt ggcgaatgaa cggctgtttg gcgaggaaca
     2461 cgctcgggtc caggtcgccg tgatcgtggt catgattgca atccggaccg tgcacatggc
     2521 cggtctgtgg cgatgtcttg ctcatgcgcc gcactataaa caggccaccg cttggtcggc
     2581 gtgaaaaagt cgcggtcagc cgtcgccaca gcccgctgcc gcgacttgtc ggtatcgagc
     2641 cgctgacatt ccgtggtaag ggtttggaat gtccgcagcc ccacagggtc ggctgtccga
     2701 gcgtggtcgc aacgcgccag ccagtccgat tcgcaagctt gagccgctcg cccgtcgcgc
     2761 aagtgatcgg ggagtgaaaa tttatcacct caacatcggc cagcctgata tcgcgactcc
     2821 gacggaattt gtcgagcacg cacggcttac accgggcgaa gtgctgagct attcaccgtc
     2881 ggcaggtctt tatccgtatc ggcaagcgct cgccaagtac tacagctcgc ttgggctggc
     2941 ggtgccgatt gaggaacgcg atctgctcat cacgacgggc ggttcggaag cgatcctgtt
     3001 tacgctgctc gcgctgtgcg atcccggcga cgaagtcatc acccccgagc cgtactaccc
     3061 gaactacggg atgtttgcgc tgatggcggg ggccgaatta aagacgctgc cgacgcggat
     3121 tgaagatggc ttccgactac cgtcaccgac ggagtttgcg cgtgcggtga cgcccaaaac
     3181 ccgcgcgatt ctgctctgca acccatcgaa tccgacggga gcggtctaca gccgcgaagc
     3241 gctggcagaa gtcgtaaagc tctgccgcga gcacaatctg acgctgatct gcgacgaggt
     3301 ctatcgcgac ttcgtctacg gcggtcagaa aaaacagagc gtgctggagc tacccgaggc
     3361 ggcagatgtc gcggtggtga ttgactcttt gtcgaagcgt ttttcggcgt gtggggcgcg
     3421 catcggctgt ctgctcagca agcgggccga catcctcgat gcgacgacgc gctttgcgat
     3481 gagccggctg tctccaccga cgctcggaca gcttggtggc atggccgcga ttgaagatcc
     3541 ggggcctttt gtcgacggca tgatcgaaca attccggcgc cgtcgtgatg tgctcaccga
     3601 agagctggcg aagctgccgg gggtgctcgc gccacaggcg caaggtgcgt tctacctgat
     3661 gaccaagctt ccggtcgact cggcggagcg cttctgctcg tggctgctcg acgatttttc
     3721 gcacaatggt gaaacggtga tgctggcacc tggtccgggt ttttacaaaa cgcccggtgc
     3781 cggtcaaagc gaagtccgca tcgcttacgt cctcggtgaa cagccgctac gacgagccgt
     3841 cgatcttctg ggacgggcac tggtcgccta tccaggccgg accgaggcac agaagccatg
     3901 accgaagcgg ttcagtccct gcaagcgctg ctggggcgga tcagctcctt tttcgacatc
     3961 ttcgatctgt cgtttatcgt ctcgggcgcg acgagcctgg ccgctttgtg tctgtactac
     4021 aaactgcatg cgcccggcgg ttttcccgaa tggctcgcca gcgtgtacgg cggcattctg
     4081 tttgtcctcg gctgctacgt ccttgggatg ctgagcttta tcttgggacg gctcttgcga
     4141 cggattgtga tgcgggtgcg cggaccattt ctgcgtcgcc aagcccgtca gataagccac
     4201 gccctgcgcg agcacaacat ctgggtcaaa gacgacgatc gcagcatgct ggcgctttgt
     4261 atctcgacgc tgctggctac accggcgcag gatacggcaa gcaaacccag cccggcaaaa
     4321 ggcaaaaagt caggcgctgc gtccgacgaa tcggcaaaga agcaaaccga cgaatcggag
     4381 acgaagcggc tggaacagac ggcgcatcgg ctttacacgc tgctgtggac gcaggttcgg
     4441 cagcagcacg atctatcgcc gtcgcatgtt ctgctcaatc gctattgggt gatggctgcg
     4501 ctctgcgacg gaatggtggt ggcatcgctc ctgtggctcg gcgtggtggt ttgttcgtgg
     4561 tcgcgtcttt cggcgatgca gcttggtttt atgggcaatc tgggaatcgc gctgtcagcc
     4621 atcgccgtcg tgctgtttgt gcgcgaggcc gaacgctaca ccgcggtcca aatcgacgac
     4681 ctgatttcga cgctggcctg ggatcgcagc cggcccccaa gtccgcccgc acaggttcag
     4741 acggcgcctt cttcgccaaa cggtgctgct tcggtgccgt cgcccgcggc agcatcgctg
     4801 tccggccagc ccacccaata acgacgcagc ggcgacagca ggagcgcctg atcaatcgca
     4861 tccagcagcg gtcgcaccgt ctctggttca atcgccgaca gcgccacagc ttcccgatcg
     4921 tgaggcagcg acgcaaaacc ttcggcatca agctggtcga tcttgttata gacaaacagc
     4981 tttggcttgt cggatagctc cagctcgaac aggatttttt cgacggagcg tcgctgttct
     5041 tccatcgccg gatcgctcgc gtcgagcaca tgcagaagca agtcggcttc cgacaattcc
     5101 tcgagcgtcg cgcgaaatgc cgtcaccaag tcctgcggca agtcccgaat gaagccgacg
     5161 gtatcggtca cgatcagctc gcgctcgtcg ggaaaacgca tccgccggct ggtcgggtcc
     5221 agtgtcgcaa acagcttgtt ttcgaccagc acctcactgc gcgtcagcgt gttgagcagc
     5281 gtcgacttgc ccgcgttggt gtacccgacg atgccaatca ccggcagcgc ccgtcgccgc
     5341 cgcattttcc gccgctgatg ccgttcttgc gaaagctggt caatttgccg ctgtagctgg
     5401 gtaatgcgtt cccgcgcccg tcgacggttg atttcgagct tggtttcgcc cggtccccgc
     5461 ccgccaatgc cgccggtcaa gcgcgacatc atcgtgttct tttcgataag ccgcggcagc
     5521 gtgtacttca gttgcgccag ttccacctgc attttgccgt cgcggctctt ggcgtgctgg
     5581 gcaaagatgt cgaggatcag catcgttcga tcgatgactt tgagcgtcgt cgcatcgctt
     5641 atcgtccggg cctgcgccgg ggtaagctcg gggtcgaaaa tcagcaccgt cgcgtcgtat
     5701 tgcagcgcgc gcagcaccac ttcgtcgagc ttgcctttgc caagcagcgt ccgcggatca
     5761 agctgggccc gcttctgggt gatcacatcg agcacttgca gtccggcggt ccgacacagc
     5821 tcgcgcagct ccgcgacgcg cgcgactgga ttgtggactt cgcctcggct ccgctcgtcg
     5881 ggtccggtgt agacgacgtg caccaccacc gctcgatcgc ggccatcgtc gaccatcgcc
     5941 gccgcttgcg ccgctttttg gtattcatct tccagcgctg gaatcagctc atcgacgacg
     6001 acttcttggc gctgcagcgg aacaggcgga agttcgcgcc atagctcacc ccgttcgttg
     6061 gggggcaaaa gatgggcgac gaacaagcgc tctgaccgtc cgccgcggcc catgccaatc
     6121 gccgccacca aatcgagccg cagcttggcc agatccagca ggtcgtcgcg gctcagcggc
     6181 tcgccgtgca gatgggtgtg aaccagacgc agaccacgca gacggccacg accggcgcgc
     6241 gcccgaccca gatcgggcaa aaacaacttt tggtgatccc cgacgatgac gtgggtgacg
     6301 atgccgccgc ggtcgatcag cacaccgacc tgccgcttca ccgtcgtcga gcactggccc
     6361 aaaaacgcgc ccagttcctc ggataccact tcgcttgggg cgacccgccg tcgataaatg
     6421 cgttcaagaa gctgtttgtc gctggccgat aggccggtca agttaccgtg gagctgctgc
     6481 atacgctctc gtcgctgcgg ttaatacatc cccagcacgt cttccatgcc gtagatgccg
     6541 ggcggctgac ccaccaccca gcgcgccgcc tgcaatgcgc cccgtgcaaa aacatcgcga
     6601 ctctgcgccc tgtgcgtcag ctccagccgc tcgccggtgc caaaaaagta cgcggtgtgt
     6661 tcgccaatca cgtcgccgcc gcgaatcgcc atgatgccaa gctcgccggc ttcccgtgga
     6721 ccgctcattc ccgatcgtcc aagccgcaga tcgcttttgc gaccgcgcgc cccttcgatc
     6781 gaatccgcca aaagcagcgc cgttcccgac ggtgcatcgc gtttccattt gtggtgaagt
     6841 tccacaattt cggcgtcgta atccggcagg gccgccgccg cccgcttgag cagcgagatc
     6901 agcacgttca cccccggtga catgttcgcc gaacagacga tcggcacccg attcgccgcc
     6961 gcatcaatcg acgccagcgc cgccgcgtgc agcgatgtcg tcccaatgac aagtggcgtg
     7021 tttgtttccg ccgctgccgc cgccaagagc gccgtcgcac caggcgtcga aaaatcgatc
     7081 atcacatcaa cattgctcag cgcgcgggcc aaatcgtcgg tcagatcgag ccccaccggc
     7141 cccagtccgc acgacggtcc aatgtcccgc cccaggtgcg gatgattggt cgcttcgacg
     7201 gctgccgtca gcatcagctt cggatcacga tcgggttcaa accccgaaag cagcgtccgc
     7261 atgatgatct gtcccatccg cccgaatgca ccgtacattc cgatgcgaat cgggatcacc
     7321 atgtgcagct cgctgccgcc cttccctgcc gtcgccgagg aaggttgaaa ctcatccgac
     7381 gactcgtcgg gattcgtgcg ctcactcggg aaagttccac tgctcatggc aaaagcccca
     7441 gcgctgtaag ctccagccgc agccgcgcct gaccaggctg gctcatcggc acaagcggca
     7501 gacgcagcac gttgtccagc tgtcccatca tgtgcagcgc cgctttgcac ggaatcgggt
     7561 tcggctcgct aaacagaagg tcgaccagcg gcacatagcg ctggtgcagt tccagcgccg
     7621 ccgcatgttc gccccggcca aagcgccgaa acacatcaac caccgcttgt ggcacgacat
     7681 tcgacagcac cgaaatcgcg ccacttgccc cgacgctgta cagcggcaga ttgagcgcgt
     7741 cttcacccga caaaagcagc agccgatcgc ccaaaagccg cgacagagcc tgcgtccgtc
     7801 cgacctgccc cgacgcctct ttgagcgcga caatttcggg ccgatcacac agccgcgcca
     7861 ccgtttccgg cagcaaatcg cagcccgtcc gccccggcac gttgtaaagc acgatcggtg
     7921 cccccagcga gcacagcgcc aaaaagtgct gatacagccc ttcctgcggc ggtttgttgt
     7981 agtacggcgt caccgccaaa agacccgtca ccccaagcga cagcgcggtc ttggccagcg
     8041 aaagtgaagt cgcggtcgac gacgaaccaa cgccagccag caccggcacc cgcccggctg
     8101 ccgcatcgac gacaatgcga atcaccttcg cttgctcgtc ggcggtcatc gccgcagctt
     8161 cccccgtcgt tccgcaggcg accagaccct gcacgccgcc ccgaacctgc gcttcgacga
     8221 gccgaaccag cgcggcttcg tctatttcgg gcaccggccc cgaccccttg tgcatcggtg
     8281 tgaccagtgc tgtaaacacg cccgaaaaac gcgccgcgct tgcttgtgcc atcgtccgct
     8341 ccttgcttgg aacttgtcgc ccgctggttc gctgtcccgt cgctggttac ttcttcttgt
     8401 tgcgcttgcg tgcttctttg gcggccttgc gcttcacttt ttcgcgcttt ttgtcgacat
     8461 tgggccgcgg cgctgagcga cgaccttcga cctgcggcat ccccgccgcc cccatgagag
     8521 cgcccaggtc cattcccgcc atttgcttga gcttcgacag cgaacgcagc cccggaatct
     8581 tgccaagcag ccctgcctgc gcgcccatcg acagcatcat ctgccgcatc agcgcgaact
     8641 tgtgcagaag ctccatcacc tcgacttctt tgcggcccga tccacgcgcg atccggcgca
     8701 cccggctttc gacgtagttg gcgacacgac gacggccttt gacctgaccg ccggaaatca
     8761 cctgttccca cgacgtttcg atgaaaagtt caggctgacg acgttcgacc atcgtcatcg
     8821 agctgatcat cgccttgatt ttcacaagct cgccgtcgtc gatgttcgcg ccttcgggta
     8881 gctgaagacc gaacatcggc atcttcgcca ggatgtcttt gagcgaacca agctgctgga
     8941 tgacgctgat ctgctcgagg aagtcctgca tatcgaactt ccctttgagc atccgcatcg
     9001 cgtcttgctc agccttctgc gtgtcgacga cctgctcgaa gtctttcatc aggccgacga
     9061 tgtcgcccat gccgaggatg cggctggcga gaccttcggg ccggaattct tcgattttgt
     9121 cgagactttc accgacgccc aggaagcgga tcggctgacc cgtgacttcc cgaatcgtca
     9181 gtgccgcacc accacgcgca tcaccgtcga gcttggtgag gataaatccg gtggtgctga
     9241 cgcggtcgtg gaacgtgcgc gccgtgttga ccgcatcttg accgatcatc gaatcgacga
     9301 cgaggaagac ttcctgaggc ttcacccgcg acttgatctg gtccagctct cgcatcagcg
     9361 gttcgtcgat cgccagtcgt ccggcggtat cgagcagcac cacttcgtag cctttggtgt
     9421 aggcgtactt ataggcctcg tcgcagattt caaccgggtt tttgccgtcg ccggtaaaca
     9481 ccgacatccc gagcttttcg ccgatcacct gaagctgctg tacggcggcg ggacgatata
     9541 cgtcggcagc gacgagaagt ggcttgcgct tgtgaatctt gtgcagccag cgcgccagct
     9601 taccgacggt ggtcgtcttg cccgaacctt gcagaccgac catcatgatg accgtcggtc
     9661 cctttttggc ccagttaaac tctgtcggtc cgccttcggg attccccatc agacccgtca
     9721 gttcttcctg acaaatcttg atgaagtgcc actcgggcgt gacctccatg ctcttgaggc
     9781 tcttttcgcc cagctttccg tcggtcggaa gctcggcttt ggcccgcagc ttgacggtct
     9841 ggcctatcgc cttgtctttg acccgcgata ggaacgagcg gaccaccggc agtgcgacgt
     9901 cagcctcgag cagcgacatc cgcacatcgc gcagtgccgc atcaatattt tcctcggtca
     9961 gctctgcctg cccggtcagc ttctcacgag ccaggcgaaa gcccttgctt agcgtctcta
    10021 acattgccgc aaggcttagc atgcctttgt cactgtcggc aacgacctcg tgcgaaaggc
    10081 tgcgtccccg cccggcagca aagccgcccc agcagaccgg aaaaacgccg cccgattggg
    10141 aatttgatat ttcgtttgct gaaagattcc ttcgctatac atttcgcttt atggaatctt
    10201 ccgatcttgg gctcttgctg gcgctcgatg cgctgctgca agaaaccagt gtcacccgcg
    10261 ccgcccgtcg catcggtctt tccaccccgg cgatgagcca cgcgctagga cgactgcggc
    10321 agcgactgtc ggacccgctg ttggttcgcg ccggacgaca gatggtgctc tcgccgcgcg
    10381 ccgagtcgct gcgcccgcag gtggctgcac tcgtcagtca ggcgcggcgc gtgatggaac
    10441 cagagcagcc gtttttgccg cagctcctca ggcgtcgctt caccgtcgtg ctgtccgact
    10501 atgtgctggc ggtgcttggg ccgacctggg acagactgct ttccgccgaa gcgccgcacc
    10561 tggagctgcg atcgattccc aacagccccg acgacgcgac gctcctgcgc gaaggcagtg
    10621 ccgacctcgc gattggcatc tacggcgtgc tgccaccgga gctgcggacg cgaccgcttt
    10681 tgaccgatcg cttcgtctgt gcggtgcgaa aagatcaccc gcagatcggc aaacggcttc
    10741 agctcgatga gttcatcgcg attccgcagg tgcagattgc gccgcgtggc aagcccggtg
    10801 gatatgtcga tgacatgctc gcagaacgtg gtctttcccg tcgggtggct cgcgccgttc
    10861 cctactttca cagcgcgcta tttttggtgt cccaaagcaa ctatctggtc acggtttcgg
    10921 aacggattgc cacgatgtac gccgattctc tgggactgcg gattctgccg ccgccgctgc
    10981 ccctgcgtcc ctatgcactg agcctgcttt ggcacccgcg ctttgatgcc gatccggcgc
    11041 atctgtggct gcgggaaaaa ctgctgggag ccgccaagct cgtcgccagt gacacccacg
    11101 cccatgcccg gacccggctt gatccaagcg atccgaacag cggccagacc cgcaaacgcc
    11161 ccggacgcag accccaaaag agctagcgaa tccctccttt tgcgaaagga atcccccctt
    11221 ttgcgaaagg aatccctcct tttgcgaaag gaatcccccc attgcgaagg gagtcgagcg
    11281 tttcattttt tgcaacatat cgtttcagac tctttcgttg tggcttgtgc tgggcggacc
    11341 gatcctaacg gaagccacgc cgccttttgc tggccaagga gagtccgatg accgccaacc
    11401 aaaatgtcac ccgcttgggc cgtcgcaagc gcatcctttt catcgccgcc aacgcatcgg
    11461 tatccccgca gaccggctgg ccgattggtt tttggtgggc cgagctgacc catccctact
    11521 gggccttcac cgaggctggc tacgaagtag aaattcgcag ccccgacggt ggatcacttg
    11581 tggcggacag cttttctgat ccagaagact cgtcgggata ttccgccgac gacatcctgt
    11641 cgctcggctt caaaaaatcg ccaaagcacg cggcactgct gcaaaacacc cagagcatca
    11701 gcgacgtgcg cgtaggcgac tacgacggcg tgttcatctg cggcggtcaa tcaccgatgg
    11761 tgacgttccg cggcaatcag aagctgcaag cgctcgtcgc cgcgttttat gaagcgggca
    11821 agatcactgc gctcgtctgc cacggcacct gcctgcttct ggaaacgcgg ctttcaaatg
    11881 gccagcttct cgtcgaaggc aagacctgga ccggctttgc gaacagcgaa gaagaatacg
    11941 ccgacagctt cgtcggtcgc cgcatccagc cattttggat tgagtccgaa gcgaggcaga
    12001 ttcccggatc taacttcgtc gtcgatcagc gcttccggga ctttgccgtg cgcgacggcc
    12061 tgctggtcac cggacagcag cagttttccg gggccgccgc tgcccgtctc gtcatcgaag
    12121 ccctgggtcg ctaacactag cggtagctgc tgttaaacca ccactccacg ccgagcccca
    12181 ggtagtgcac aagcgagtgg ccggcgcgaa catcctgcgg ctcgaacagg tagtactgcg
    12241 cgtcgctgct gagccagctt agggtgtaaa tgccgtacag aaatgggcgg gaataggtgc
    12301 cacgaccgag cggcgcgacg atgggaatca cgctggcgcg ggcgaccatc ggctttaggt
    12361 aacgtccgct gtcctgatcc accgttgtcg gtcgccgcac ttgatacgac agctcgacgg
    12421 cggtgtggat gtacttggta aagtaaatcg tcggacgcag cgccacggcg ccttcgctaa
    12481 agccactgtc ttcggcttgg ctggcgtcgg aaaaacggcg gaaataacca cctgccatca
    12541 cgccgatcgc ttgcgattca tagttggccg acaaagcgag cgtgctttct tttgcgccaa
    12601 acacacggac atcgctgccg aaaccaaacg gcttgcccaa atcaccaaac gcgccaagtc
    12661 cgccggcctg acggaaaaac aggttcgcaa acacaaagct gcgcagccag ccgcccactt
    12721 gcgcgccgat cacatagccg tagtcgtcgg gaagtggctg tggcggcaga cccgtcagct
    12781 ccggggcgcg ctcgcctttg ggcagatagt gaaattcgcc gtaaaggatg aacttggcgt
    12841 tcggcagatc cgacaggttg cgcagaaact gcgtcgcttt tgccgacacc accaagcgct
    12901 ggcgatcgag cgttgtcacg gtggtcgtcg cggactgctg gctcaggatc gtcgtcgagc
    12961 ccggcagtcg ccgcgccgga acctgaatct gctgaaactg ataaaaatcc gcgagtcgat
    13021 tcatccccag gtgaatctgc agcaacgtcc gcttctgatt gagcgcatag gctgcgccac
    13081 caccgacggt gtttaggttg tcgagcggcc aaaagtcgaa cagatacacg tcgtcgccgc
    13141 gatacatccg cgatccggcc cacagcgata ggccatcgac ccccaggttt tcgacttcgg
    13201 cgtaggcgtt gcggatcgca aagttctgcg aaaaattccc ggtcgcgtga aacagatcgc
    13261 ccgagtagcc agggacgaac accacacgcc agcgcttcgc cggatcgtcg ccgatggtgc
    13321 ctccgtaata gaagttgagt tctaggtacg cgggtttttc cagacgggaa ccaaacgaca
    13381 cgacgttgac gccatggccg ctgtgcccgc gcagatcact gccgatgccg acgcgaccgt
    13441 agctgccgaa gtcaaaaccc gacgcggtcg ctgatgcggg aggcgggctt cccgacgaac
    13501 caaactggct gtgggccgat gctttgcgtg ccccactggc gtcgtcaaaa gccggcgttc
    13561 cgtccgtggc gtgagacgcg gtcttgtcag cactcggggt cgcggggctt tcggcgtgcg
    13621 cgatgggcca aggcacaagc acagcgcaca gcggagcggc gacgagccag cgacgagttt
    13681 ttgggccaag cataagcgac cagagcctcc agtttgtggg caaacggtgg gccaagaaga
    13741 ccaaacgtgc gcacaggtag gcaagcccca atcttgcgga attgtcgtca cggatgagat
    13801 cggtggacgg cgccaaatca gcgcagcgca aacaacataa agcgaccgac gtgggcgacg
    13861 aagcaggcgg cgcggacgtt gggcacttga cggcccattt tgcctgggcc tacgttgcaa
    13921 agagaggcac cggaaatgcc taaaaaggag cttgcaatga tgatgacgaa gcgtgttttg
    13981 aacgtggcga tggggctttt gctttcgacc gcagcgtgtg gcgtgacggc agatggcaca
    14041 gccggcgggg tggccgacgg agtgatcgat gaatcgctgc gcacgacgga tgtgccggca
    14101 gaagaagcgg atctgaccgg gacggccaac tcgacgtttt accgggtgac gcatcccgat
    14161 ctgcgacgct gcgtgtatcc gctgtgcggc ggctacttta ccgagcgtgt aaaccaaaag
    14221 acggcgacgt gttcggacgg caccgcgcag cctgaatgcc ggattttgga gtttgactat
    14281 gcggcgctca agctcgatgc cacgtcgcag gccaagctga cttcggcggt gcaaaatggc
    14341 caagcgctgc tgcgtgggac catcggcaag acggcaccga ttgccggcaa aacgtatgac
    14401 aagctggtgg tgtcggaagc gtgggtggcg cgggcactgt cgacgccgag cggaacctat
    14461 gcgcgatcga aggagctgcc gctggcctgt gctggctgtc cgacgttttt gcatgccaat
    14521 ctgaacaaga ccacgtcgca gcgatcgcac acggtgctgt ttgatgcgac caagttttcg
    14581 tcgacgctca cggctgaact gcaaaaagcg atgcagagtc tgcccgaagg cgtgctcacc
    14641 gccggcacat tgacgacaag cggtagccgc caggtgtgga acgtcagcga agcgtacacg
    14701 cggtttgtcg cgtcgggacc acggcttggc aagctggggg aaagctgcgg ttcgcgcggt
    14761 ttgccgttcg actgcgaaac cggtctgttc tgtcagcgcc cggacaccgc catctgtgga
    14821 cgcgccgacg caccgggcac ctgccagaaa aaacccgaag cctgtaccgc ggtctacatg
    14881 ccggtctgcg gttgtgacgg caaaacctat tccaaccgct gcgtcgctta tagctatggc
    14941 gtttcggtcg actacgacgg cgcctgcaag accccgtaag ccaaaccgac cgagccaccc
    15001 ggttcccggt tagctgccgg gccggggcaa aagcggcagc agctgattcc agatgtgctg
    15061 cgcgacttct tgtttgggca taagcggcag cggaaccacg tcgatgtcgt cgccccgccg
    15121 caccaggatc gtcacccggt ttgtatccga accaaagccg ctgcccgctt ctgccacgtt
    15181 gttggcaatc agcacatcac agcctttttc acgcagcttg cgccgagcgt aggcgtccag
    15241 atcctgcgtt tcggcagcaa acccgacgag cagcggcgcg attccctgtt tcgcgacccc
    15301 aagcgcccgc aagatgtccg gattttccac aagcgcgatc gtcggtgagg tgccaagatc
    15361 ctgctttttg cgcttgtgcg gtgaaacctc gctcggacgg tgatcggcga cagccgctgc
    15421 catgacgata cagtccggcg gcggattctg ttcgacaagc ggaagcacgg cctgcgccat
    15481 ctcgcgcgca ctttcgacat ttttccgcgt cactccgcgt ggggtttcaa gcgcgaccgg
    15541 acctgcaaca agcgtcacct gtgcccctcg acgagccgcc gattccgcga gcgcaaagcc
    15601 cattttcccc gaagatcgat tgccgataaa acgcaccgga tcaatcgctt cgtaagtcgg
    15661 tcccgccgtc accacgatgt gctttccgac cagatcgcgc ggcccgcttt cctgcacaat
    15721 ctgcgcaagc gaaagcacga tgtcgctcgg ctcggtcatt cttccttggc ccacccaacc
    15781 acaagccagc tcgccggctg ccggcccgac gatgtgcgca ccaaacgacc gcagctgcgc
    15841 gatgtgatgc tggacctggg gtttgttcca catgttgacg ttcatcgccg gacagatcac
    15901 aagcggagcc tgacaggcga gcgccaccgt cgacagcaaa tccgtagcga gtccaagcga
    15961 cagctttgca atcacatccg ccgtcgccgg tgcaatgacc aacagatcgg cggaatccgc
    16021 cagattgatg tggccgattt ccgattcctg cgtccgatcg aaaagctctg tcgccaccgc
    16081 gtttcccgac agtgtctgca aaagcagggg cgtcacaaac tgctgcgccg cgtgtgtcat
    16141 gatcacacgg accgaagccc ccgctttgcg cagaagccgc gtcagttccg ccgctttgta
    16201 ggccgcaatg ccgccgctta tccccagcac cacgttgcgg ttgtgaagcg gcaaggaagg
    16261 atcagaagtt gcatccatcg ccccacttta gcagcacgag tcccgtcgtc gcagttttct
    16321 ctgtggtttc gctgcccaaa cagccgtgcg gcgggtccaa gtcttacgta acatgctagg
    16381 ttgtgatgcg cagagggatc catcgccaaa ggggccgcgc atgagttctg ccttgccggc
    16441 aacgtcttca gcgcgccaca gactgtggct gcgggtgctg ggaatcctgt tgttctggat
    16501 ctgtagcgtc cgatctgccg ccgcacttcc cgacgagacg tggcttgtgg tggttggcaa
    16561 caatcgcggc gatgccagcg aagtcgaact tctgtacggt gaacgcgacg ccaaagaact
    16621 ggccgaagtg ctgcgcacgc aaggcaacat cgcatcggac agagtgcgaa ttctcctcga
    16681 tgaggatgcc gaaacagtgc gccgcacgct ggtcggaatc aacacccagc tgcgcagtca
    16741 gaccgcgaaa ggaaacccct ctgccttgct ggtgttttat tccggacacg cagatgcaga
    16801 tgcgttgcat ttgcgcggaa cgcggcttcc tatcgacgaa ctgcggggtc tggtcagcag
    16861 ctcgccagcg acgatgcgaa tcctgattgt ggatgcgtgt cgttccggtg cagtcacccg
    16921 cgtaaaggga atcaagagcg cccaagagtt tgtcatcaag atggaagatc gggtcgaagc
    16981 cgaaggaacg gcgatgatct ccagcagcac cgcaggagag tccagtcagg aatccgaccg
    17041 cctgcgcgct tcgttttttt cccatcacct gatcaatgcg ctgcgcggtg cagcagacaa
    17101 aaacggcgac gggaaagtaa cgctttcaga ggcctatgct tacagctatg cacagacgct
    17161 ccgatcgagc ggacagacgc tccaactgca acatccgacc tacgcatacg atgtaaaagg
    17221 aagcggtgat ctggtgctga ccacgatcca aagcgcgcag cacaacgtgg gtcgcttgcg
    17281 actgtcttcc gccagtacgt acctcatcac cgaagaaaag gagaccggcg cgatcgttgc
    17341 agagcttagc accgcgcgcg atcatgcggt ggtcgcactg ccgcgcggaa agtactttgt
    17401 tcagcgacgt ggaagtcacg aatatcgcga atatctggca tcgcttctgc cggcccagga
    17461 aatcgatctg gaaacgattc cttatcgctc ggttcgatac gatcagctgg tgcgcaaacg
    17521 gggtggcagc cgcgcatctg ttcatggtgc acatctgcta ttgggaatgc gcgggtcgat
    17581 cttgccaggg gaaacggtca cgccgcattt gctgcttggt tatgcagccg atctgccctg
    17641 gctgtccgtc ggaacacgcc tgcgtggcag cacaacgtcc tatcaggcca tcgatggcgg
    17701 ctacacggcg cgacaccacg agctgggtct ttctgttctg ctgcagcgct atgtcgatct
    17761 gccgtggtgt agcttggcat ttggggtgca aatggaaggc acatggtatg cgcagcgcta
    17821 ttcccaaaca ctgcgaatga tctcagcgcg tgacaccttg ggaatcggtt tttccgcgct
    17881 gtttgccgtc gaaaggcagc ttctgcacgg aatcggaatc cgcatcgaag gcggcccgat
    17941 ggccaacctc tttcgccacg ttgaaagtca gtttggcgaa gccacagaca ccgtgacgcg
    18001 atcggtcttt acgttctggc tggccggagg tgcaacatgg cgtttctgaa gccgcagtct
    18061 gcctgcgtta gcagacgctg cgaaatgatc cgcggaatga tctgcggaat catcggggcc
    18121 acactctggg gatgcgggga cggactcacg gatgccgact atcgtggcac tccgctttgg
    18181 cgcatgagcg gcggtctgga acagtccagc tttgacacca gcgtgtatcc cacggtgcgg
    18241 attgctctgt tttggaaccc aagcggagag acgccgcgcc cagcatctca gctcgtcgaa
    18301 cagaccagcg cgtcccaagc ggttgcggtc ggcaatccgt acatcttgaa catgtttgat
    18361 ctgccaggtg ctgaccacat cgcacgcacc tacgatggtc gcagccgagg cttcggaatc
    18421 ggcagggtgc tgggttacgc cgacttggat gcaaacggac gctattccga cggcgacaag
    18481 tttgtcagtc tgtggtctga tgtggcgctt ttttatgcag cttctgatct gccaagctgg
    18541 gcatctccga cgagtggagc cctgtctgcc ggattttatc agctgctttt gccgcagcgc
    18601 tgtgacaagc cgattcccac cccaaccgat tccggaacct gcggcatcac gctgggaaaa
    18661 aagtgcggca ccgacgcaga ctgtggaacc ggcggaacct gtctgcgcga aaccaacatt
    18721 ccttggccca ctggatactg cgcagtggca aacacgccct ctgcaagctg tcgtcccgca
    18781 gcagcggctt actatgggat tccgcaagac attcccaatc ctcccactgc gcgcggatac
    18841 tatctaaaac catgtcaaag tgatcaggac tgcgtgacac aaacccagcg tgatgcgggt
    18901 gtgtaccgct gtgacttggg tctgtttgcc tgcacgccca gcgtcggtgg aaaaatgagt
    18961 gtgggcggaa tccgcgacat cgaagcagag cttacgcgcc agccgttttg catgacaaac
    19021 taaaccggct gcgcgcaaaa gtacgccgcg tgataaacgg gcgttgtcac tgcgcttgcg
    19081 tggttccgaa cagactgtgc gcgcagcagc ttgggcgtga tctaaaagct actggtcccc
    19141 acgcggattg atctgtagtt ccttgagcac ttcgatggct ccctcgatgc ggagcatggt
    19201 ctgctggact tgtttctgtt tcgcttccag ctcgaccagc acgcgctgtc cggcttcata
    19261 ttcagcaaca agcgaggcca agcgctgggc aattctgtcg ttcatgatct tcctcccgca
    19321 accgttttag ggagtcccaa gccgtaagcc caagcgacac tcccaagtga ctgattgtac
    19381 agcggtgggg cgcaaaaaag cagagaaacg cgacagcagg tttcgtcgga aacgaccgga
    19441 cggagccgtt ttgcacatgg tctacttggg atacaaaccg cttttggtgc ggcctgtggt
    19501 tttgtttcgc agccacttgg ttttgaggag ttcatctgat gtccagcaag atcatcgacg
    19561 acttgacgca tcttcccaac atcattgccg gtctgggtct tggcatcgca caagcgcagt
    19621 ccaagatgga tctcgactac cttcagtcgc tcgaacgatt ggtggtgatg gcaaaatcgc
    19681 tgctcggcgg gcagaaagca tccagcgccg gcaacgtacc gacgacggaa gaagatcagc
    19741 gcaagctgga acagttccag ggtgtggtca aagactttct gacggtgctc gcgccgactc
    19801 gctatcagtt tacggaaacc acgctgacgg taaaactaga gctttcgcag cacctggatg
    19861 tgtcgggatc ggcggggctg tccgcaggca ttggcgcggt ggcgatcaat gcatcgctgg
    19921 cggttggtta cggcagtgat tatcgcggcg cggcggaatg caaaaccgtc ctgcatgcgg
    19981 ttccgaccga cgcaaacacc atgcgaacgc tgcttgatcg ggcaaaccag cttggcacca
    20041 aagagctgtc tatgccgcag cgcacccagg tcgattccgc tgtccaaaac caagcaagct
    20101 cggtgtttga aaaaatggtt gggtatgccc cagcaacggt aaaagacagc gcaactgcat
    20161 ctgcaacggc tgcgaccgga agcgaaaacc ggggctaatc ccgcgccacg acttgggaat
    20221 ttcgcgtcgg cctgttaaca tgccgcgaca tgcccgacga ccctgacgga cccaattccc
    20281 atccaaacga ccgaaatccc ccctttgccg aacagcgcat cttggttttg cacaacgccg
    20341 atttcgggga acagcccgac ggtgacgagt cctacgaatc ccgcgccgat gtcgccaacc
    20401 aagcccatga tgtcgcgcgg gcactggtga cgcgcggaca cttcgtcgat gtggtgggaa
    20461 ttgatcggga tgagcttcct tctctgatgg aacggctccg cacggaccca ccggatctgg
    20521 tcttcaactt ggtggaatcg ctggcgatgc aagatgaaaa ctgcgtcgca gcgacggcga
    20581 tcttggaact gtttggcatt cctttcacgg gttcaggaac gctcgcgatg tcgctaagct
    20641 gtgacaagca gcgcagcaaa catctgctcc gcggagacgg cattccaacc ccacgcgccg
    20701 cagtcgtcga accgcagttt cgttcgcgga tttctgacct aaacgccgtc gctgcggtcg
    20761 gttatccagc gattgtgaag ctgctgtctg aacatggctc gcaaggtctg tcgttcgatt
    20821 ccgtggtgta tgatgaggaa tcgcttatcc gtcagctgcg ggtgatgcgc gattacttcc
    20881 ctggtcgacg aattttggtc gagcagtaca tcgacggacg cgagctcaat gtcgggctgc
    20941 ttggtgatcg tctgttgccg gtgtgtgaaa tcgatctgtc ggcgattccg accggcaagc
    21001 cgcggattgt gacgtatacc ggcaagtggc gacccgaaag cgaagaatac cggatgatgc
    21061 cgtcgtcgaa agtggcggtc ggactgtccg cggcgctcac cgaacgcatc cagcaggtcg
    21121 cgcagcggag cttttccgca ttgggtgtca cggactacgg acgggtcgac attcgcttgt
    21181 cacaagatgg gattccctac gtgctcgaag taaatcccaa ctgcgatatt tccgacggag
    21241 caggtctttc gcgcgcggcg ctggcagcgg gaattcccta cgatgcactg atcgagcaag
    21301 tggcctactc cgcccggcag cgcttttctg gcggcattac accaccgacg gaaaaaaagc
    21361 gcgcgactgg atcgtaaccc catcaagcgg cgaaatggcc gcttagcgca gcacaaagat
    21421 cgcgccgtgc tgcccgccaa tccacagccg acgtccgtcg tagatcaagc tggttaggtt
    21481 ttggtttgtc ccgcttggaa ctttcgacca cgactgtcca tcgaaacgca gcacggtgcc
    21541 gcgatcgccg acggcccagg cttctttggc gctgcggcaa agcacccgcc acaggttgat
    21601 gtcgtcgggg gctttgacac gaccccagcg cgccccatcc cagtgggtga tgacgccttt
    21661 gacaccgacg gcccagacat cccgcgcacc gcagcttgcc accgacgtaa gtcgatcggt
    21721 ttcgccggtc gtgatgctgc cccactgctc gccgtcgtaa tgaatcgatt cgccgttgtc
    21781 gcccaccgcc cagatgtcgc gcgccgagct gccatccatc gccgtcaggt tttcggtcgt
    21841 cggcttgctg agaaccgccc actcttggcc ggtgtaatgg acgacggtac cggattgacc
    21901 gacggcccag atgtcccgcg ctgagctggc ccacgccgcg gtaagcgagc ttgcgccacg
    21961 tcccgccaac agcgtgtctt gccacttttt gccatcgaag tgacggacgg tggtgccggt
    22021 tccgaacgcc cagacatcgc tgccggaaaa accaagcagg gtccgcgtct gcgcaaagcc
    22081 ataaccgggc accttttctg ctttccagcc gccgtcgcga aaacggagca gctcgctgcc
    22141 ttgcaccgcc cagacttcac ttggaccttt ggcccacatc gcactgaccg ggctcgtcgt
    22201 aggatcggtt tcctttttcc actctttgcc gccatagtga gcgataagtc cgctcggccc
    22261 gacggcccag atgtcatttg gactgctgcc ccacagggcg tggatggcca ggctgtcttt
    22321 ttggactttc cactcatttc catcgaaatg atatattctc cccgtcgctg atccggccca
    22381 agcgtcggtc gcgctgtagc cccaaagcgc gtccattccg cttgccagac tgggcccgaa
    22441 cgactcgccc gatccaccgt tttgcgagcg agacagccga ccactccagg tcgatccgct
    22501 ggccagccag acttcgcgcg cacgatgtcc ccacaccgcg cgaatgtcgt tgtacgcacc
    22561 gctcgacacg gtcttccagt cgctgccgtc gaagtggcgg accagaccgc tggaacccga
    22621 aatccagaca tcgctgctgt caaagccgta caccgaaaac accgtcgagt agtcactgac
    22681 cgaagcgctg gctttcttta tcgacttgcc gtcaaagtga agcacgctgc cacgccggcc
    22741 caccatccag acatcgccgg caccgctcgc ccacgtcgaa agcagcgttt cttttgtgcc
    22801 actgtcgatg cgattccagt cgctgccatc gaagtgcaga accaaacctt cgtcgcccac
    22861 cgcaaaagcg tctttggccc cgacgcagta gaccccgtgc aagtccaagg tcgttccgct
    22921 tggcgacttg ttccagctct tgccgtcgta gtgcaggatg gttccgtagt cacccaccgc
    22981 ccatatgaac ttgctgctga tgccggagat cgcgcgcagc gtgttgcctt gcggtgtcgg
    23041 tcgttgccag cacactccgc ccgctgaaca gacctggctg ctcagcccat ctgcgcgtcc
    23101 acaagccgac aaaagcggag cgatcgcgag cgcagcgcag acaagccagc gacggcgaaa
    23161 cgtctgtctc gaggtcataa ggagatcact ataccacggg gtaaaaagcg caatccgtcc
    23221 gcaccgactg accaagcatg caccctgtgc ttgattcgga atttttttgt ctcgtatgat
    23281 gagcattcat gcgtcgcgtc ggccaagacc tcatcgatat tctcaagtgg gtgggctttg
    23341 cgccgtggtc gctgttttgg atgacctcgg cgatcaccta cgggatcatc ttccgaaacg
    23401 atccgcacac cccgctttcg tgggcgcgcc gcttctggag ccccggcgct gcggtcatct
    23461 ctggctgtca gctgacccgc catccgggct ttgttcccga accaggcaag ccgtacattt
    23521 tcatgatgaa ccaccagtcg atgttcgaca tcgtctgcgc gttcattcac gttccggtaa
    23581 acctgcgctt cattgccaaa aaagtcctcc agtcgattcc atttttgggc tggtacatga
    23641 ctgtgaccgg aatgatcttc gtcgaccgtc gcaagcgcga tcaggcactc aaaagcctgg
    23701 aagccgcctg cgagcgaatc cgcagcggca ttcccatttt ggtgtatccc gagggtacac
    23761 gctccaaaga cggatcgatc ctgccgttca aaaaaggccc gttcatgttc gcgctgcaag
    23821 ccggcgtgcc catcgttccg gtcgcgatca acggaagccg tggcatcttg cagccgggaa
    23881 gcccctttgt cagagccggt cacatcgaca tcgtgatggg cgaaccgatc cccaccgctg
    23941 gcctggacaa gcaagatcgc gatgccctga tgcggcgtgt tcgcgacgcg atcatcgact
    24001 tgcaggtctc gattggcggc cccggcggag acaaagaacg ctacttggca gccgaaggca
    24061 aagaaggcgt cgccgatccc accatcgatt cgatcggccc cgattcatcc gtttgacaca
    24121 agggcggcag cttcacatcg ccgaggaaac gctcgcgctc ctctcgattc aaatctcgac
    24181 ccgccagtcg acaggccagc gcaagccacg acgacaccgc cagatcccac acccgcagcg
    24241 tgccatcatg actgaccgaa taggcccgct gggaatttgg actaagtcca actgcggaaa
    24301 tgctccggac atgcccgcga agccccggtc caatcgactg tagcgtcgtc aaatcccaca
    24361 gccgcagcgt ttgatcttcg ctcgccgata cggcccgcag gccatcgcga ctgaccgcga
    24421 tcccggtgat tcgatcgacg tgcccacgca ggggttcgcc ttgggttttt cccgtcgcca
    24481 gatcccacag ccgcagcgtc ttgtcttcac tgctgctaag taggtgctcg ccatcgggaa
    24541 ggaagcggca gtcggtaacg gcgcggctgt gaccttgcaa cattgtctgt gtccgtccgc
    24601 tgcccagttc ccagatccgg accgtctgat cttggctggc cgtacacagc cgtttgccgt
    24661 cgctggacag acccatgccc cagatcgcgc cgctgtgccc ttgcagcgtc tgttccgctg
    24721 ggcgtccgct ttttgcgtcg aagctgcgca ccgcaaagtc gaagcccgcc gaataaagcc
    24781 gtgtcccatc cacagaaaac acaagcgcac tggtcaagtc gccgcccgtt gacagcgtct
    24841 tggccgtccc cgtcgccaca tcccacagcc gcaccgtctt gtcaaagccc gccgatgcca
    24901 gcttggtgcc gtcttccgaa aacgccaccg cgctgatgct gccgtcgtgg ccgcgcaaca
    24961 ccttgtcagg ctgcagaccg cccgcgtcga cccgaaacag cgtgatctgc ttgtcccagc
    25021 ccccgaccgc cagccacttg ccctcgcgat cacttgccag gctcgaaagc tcactcgaaa
    25081 gcacatgcga ctgtcgttcc agcggcgagc gctcgtccag ctcgacttcc agcagccgac
    25141 cttcttcgtt gccggtccac accgatcgca agctcggatg cagcaccagc gcctgaagcg
    25201 ccccttgccg cggatgcagc ggacgaccga tctgctggcc ggtttcaggc tgccatttgc
    25261 gcagatcccc gtcccagctt gccgacacaa gcgattgccg cgacggatcg gccacaagac
    25321 ctgtcagcga aagaccatgc ccatcgatcg gcttgccctg cggttttccc gtctgaacat
    25381 cccacaaaag cagatgccca tcccagtcac ccgccgccgc cagcttgccg acgaatgcca
    25441 ccgctcgcac cgtcttctgc gcccccaaaa gtacgtgcag ccgccgcgcc gctttggcat
    25501 cccacagccg caccgtctga tcgtcgctgc ccgacagcaa ataccgactg tcgggactgt
    25561 aggtcagcgc catcaccgcc cgctgatgtt cgcgcagcgg cggaccgacc agctcgccgg
    25621 ttttttgatc ccagagcagg atcattccat cgtcgctgcc cgacgccaaa taacgaccat
    25681 ctggactcgc agaaagcgaa cgcaccgccg tgccatgacc caaaagcggc cccccgaccg
    25741 cctgcccaag cgagtcctgg gattcggtgt tccacgcccg aaccagcttg tcttcccctg
    25801 ccgaaaacag ccgtttcccg tcgctggaaa atgccagagc cgacgtcgtt cccgtgtgcg
    25861 cgcgccacgg cttgtgcaaa agtcgtcccg actttgcatc caaaagccac agcgtcccgt
    25921 cgtcaaaacc cgccgccagc cgattcccat ctgacgactg agccagcgag cgcagcggaa
    25981 ctccgtcggt gtggatcatg cgaaccagcg gcgctcgtct ttgcaaaagc tgcaaaagca
    26041 gaccttccgc ttcggcacca ccttgcaggg tgaaagccgc gaccccgagc agcaccgcca
    26101 gatcaggcgc actgtcctgt cgaacttgcg cctgcgccgt caaaaaacgc agcgtcgcca
    26161 gccgctgttt gtccgtcgcc agcttgcgcg cctcggcttc cgattccgcc cagtgtttgg
    26221 cttcctcttc gcgcacacgc gcccagcgcc tttcgcgcag cgcccaaaac gacagcgccg
    26281 tcgccgatcc cgccaaaagc agcgtcatcg tcgccaccgt cttccacagg cgcgcccgcc
    26341 ctttttcccg ctcttgctcg aacagcagtt tttggcgcgc tttttcgatc gcagcatcgc
    26401 gcgcagcctg ctcggtctgc tcgacgtgac gacggctttc gctgaccagc gcacgaatcg
    26461 tttcacttac gcccagctcc tgcgcgtcgg gactttgcaa aaactgttcg gcttcttttg
    26521 tctccaccgc gtcgagcaga ccgccgccac gactcgccag ccgctgccaa tcatcggctt
    26581 gctgttcgag ccttcgtcgc acccgctcgg cttggcgcag ctcggacagc cagcgacgca
    26641 gccgcggcca cgcccgcaac atcacttcgt gcgccagatc gaccagcaca acctgtggcg
    26701 actcgctttg attgtttttg ccgtcgccac cggcatccgg tccagcgctc ttggtgacaa
    26761 gtcgcgcccg caccagtcgt tcgatcagcg cctcggtctg ggatcgatcg tcgctcgtcg
    26821 aaaccagcgc cgtccatggc tgctgccgcc tggtatgcgg cctgccttcg ccaaactgcg
    26881 tcaaccgcag cagcgtgcgt cgacaaagcc gctgctcgcc gaccgaaagt tctgcccaca
    26941 cctgctcggc gtgcagcgtc agcgccgccc ccaggccact gacctgggca gctcgcggca
    27001 gcgacgggtc cacaagctgg gccaagtcgt catacgcccg cagcgtcagc ttgcgacctt
    27061 tttgtcgttc ccacaaaagc acgagcgtct cttgcagaag cggcagcgcc cccggctcat
    27121 cagccacttc ggcaagcaag cgctgcacaa gcgcgggctc cacccgcaga ccctgctgcg
    27181 ccgccggctc gacgatcgct tcgaccagcc gatcgcccga aagcggcgcc acttccagac
    27241 gctgcgaagg gtcaatgggc cacagccgac tcgccatcag ctcagggaaa aagtcggcac
    27301 gaagcaccag caccagcgcg acggagggca gtgcagaaag cgccgccgtc ttggcaaaaa
    27361 agtcctgctg ttcgctgcgc gggctcgaca aaaacagctc ttctagctga tcgatgatca
    27421 agaccagtcg ctgcgccggt gctgtggcca gcaaactttg gactgaatct aaactcggca
    27481 gcttggcctg gcccggtcgc acgagccgca cgacaaacgc gggtccaagc gttggcacaa
    27541 gccccgcctg cagaagcgac gtcttccccg atcccgatgc gccgatcacc agcagcaagc
    27601 gatcccggcg cacatgttcg gtcagccagt cgatttcact gcgccgcccg aaaaacagcc
    27661 gcccttgatc ggctgcaaac gaaaccagac ctggatacgg acagcgcggc tccgctttgc
    27721 caattccccg ctcggcaagt gctgcaaaca cacggtcaat cggacacgcg aacacactgc
    27781 cagcgacgga atttcccgca tcatcgagcg tcgcccagcg caaaagtccc gccaccgcgc
    27841 catcgaccca caccggcgcg cccgaaagac ccgccaaagg cgcgccccga ccagcggcgg
    27901 cttcatcaga aaaaagctga atcgccggac tttggccgac ctggcttagc gtcgaacgaa
    27961 cctggccgct gacccacaga ccgtcgggac gcgcttcggg aaaacccacc gacagccacg
    28021 acagcggacc gggggataag tcgtcttcag acagtggcaa gagcaaaagc ggcgcaatcg
    28081 gctgacctgc cacatcttga ggtacttcaa taagtcgaag cagcgcccag tccgcttggc
    28141 gatcgaaagc aaccagctct gcagcgatgg tctgcgaggg aaaatgcagc cgaactgagc
    28201 caagagcgac cggactgtcg gcatgccgat cggcgacaac gtgaagcgcg gtcagcgcgt
    28261 gctgggcagc gacaagaaat gccgtgccgc gctgctcgcc taggtgggtc gatacttcga
    28321 cgcgagcgac ggcctgaaaa agccgtgtcc gctcggactg ttgcggcatc agagcggggc
    28381 cccgctttgg tacgtttcga cggacgcttc gacgacctga tcgcccgcaa gctccagccg
    28441 cacgtaggtg ggccgtcgca caatcagccg atcgtaccgc ctatcggcca tgtctgcgac
    28501 gagctgctgc agcgccgccg ccgaaacctg cgggtcgtcg gcaaatacct cgtcgggaac
    28561 ccgcatcaga tcggcccacg tcccactgtt caaatagcgt ccgcccccgt cgagtggcag
    28621 gttgcgtgcc agatgcgtat gccccatcac gatgtaacgg aaaggtgaaa tccgggccag
    28681 ctccttcgcg gcctcgaaca acggctcccc tttcggactc ttgtcgatcg caaacagcga
    28741 cggatcgcaa aacgcccgca gcgccttgtg cagcgctttg agccgagatt ccggcgcgcc
    28801 acccccggtc agaagcagct tggcccacga cagaccgcct gaccacttcg agtcgcgacc
    28861 cggcgcagga tcggttcccc gtggacgcgc cgcaatgtcg ccccgctcgc cgccccgtgt
    28921 cacaaacgcc atgtcgcgcg gcgtcgatgg tgcaccgccg atctggctca aaaacgggct
    28981 cacttcggct tccccgagcg actcaccaag catcgccaaa agctcggctt ccccgtcgga
    29041 catcggcgtg cggaacccca gttcaccgtc cggttgcggc ccgcgcacgt tcggcacatg
    29101 cgcgctgatg tcgcccgatc gcgtcggcag cgccggcttg gacagtcgaa gctcaccggc
    29161 ggagatcgcc agcttggcca gcttggcaat ctggctgcgc gcccccggtt ccagcgcgag
    29221 caagagcggc agcgccgcgg tgttttccgg tttcagcaag tcaatgaacg gatggctgcg
    29281 cttgaccggg ttcatcacct cggcgacaag ctggctgccc gctggggccg gaaaccgcag
    29341 cgacggagga atggcttggc cgcgcgactt gagcgaacag acccgtcgca gcgcatcgta
    29401 gtcgatcgca ttccagccgt cgtagcgatt gccatgttcg atcagcgcgt cgcccacagc
    29461 gtaggcctcg ccgtcgtaaa tgaagcgcag accgacatgc gacggcaccc cgagcaggtg
    29521 tttgagcgcc tgccgaaccc gcggataaca cagctcgatg tcgtgattgc cgagcaggat
    29581 cgtcagcgta tgaccgcgcg cggaaaagcg acgcagcgcc gcaaagacat ccggactgcg
    29641 catcgccacg gtgtgcagca attgttctgc cacggcatcg tcttcgcgca gcgctttcca
    29701 aaccggatcg ccgttttcgc cagttccatc tgattccgcc aaaaaatcga cgaaatcgcc
    29761 ggcaatcacc agttcttcgc ccccattgtc gggatttcgc gctgccaggc gatcaatcag
    29821 ctcggcaagc gctgcagtct gcgtacaaat ttggaagccg cgctcgcctg ccgccgcttg
    29881 tgccccgccc aggtggagat cggacacgat gtaaaccctg cgcgtggctc ccgtcataac
    29941 cgcttctccc gttgcgttct gatctgtaga tgaagccgcg ccatcttaca cccgcctacg
    30001 ccggatcgcg gtgtcggtcg aaaaccaagt gcgcggtgtt cctcgcagcc catccaacaa
    30061 atgttatttt gttggttcat ttactaaatt tacatcggct catcggatgc tgggcgcgga
    30121 caactggccg gacctgtgga cggcaagcac ctttgcgagc ggacgccgat aaccagatcg
    30181 attcaactgg gagcaagtgc aatggccaat acgggtcagc gcagggaagc gtttggcgcc
    30241 tactatttcg tcgttgagat cgagggtgaa cggttcgcgc acttccgttc tgcaagcggt
    30301 ctcaagtccg agggagaagt ggttccgctg atggaaggtg gttggaacgc cgctgagcac
    30361 aagctcgtcg gaagaaccaa gtacccgaac atcattttga agcaaggttc cgccggcccc
    30421 gagatgtggc ggaagcggca gcgcttcatc agcgacacca cgggcgatct gcgtcgtttc
    30481 gacggtgcga tcatccagat gggccccggt ggcagagcgg tcgcgacgtg gacgttcacc
    30541 aacggctggg tctgcaaatg ggaagcaccc gattgggacg ccggcaaaaa cgagatcgcg
    30601 atcgagacca ttgaaatcgc ccacgaaggc ctggcgctca atggatcggg cccgacgccg
    30661 ccacgctcga aacactcgtc gggggccaag agcgccgctt cgtcgagtgc agcgaagcaa
    30721 gcggcggcgg cggccaaaag tggcgcagga gccgcagcca aaggcgctgc cgcgacggcg
    30781 aaaaatgccg ccaagggtgc taccgacgcg gccaaaaacg cagccaaggg cgctgccgat
    30841 gccgccaaga ctgcggccaa gagcgctacc gacgcggcca aagacgcagc caagagcgcc
    30901 gaaaaagcgg ctggcgcagc ggcgaacaag gcggttgccg acgcgaccaa agatgtcgcc
    30961 aaagcggctg gcaaggccgg tgtcgacaac ttcaagccgg aagtcaccgt cggcaaagac
    31021 ggcatccacg cccacgtcga aggcgacttc aagaaaaacg tcgctggcgt cccggtgagt
    31081 ggtcacgtat cggcagatgc ggtggcaaac aagagcggcg tcaaagcgac ggcgagcggt
    31141 tcagccaaag ccgaagccgg tccggtcaag gcggaagcca aggccgacgc gggcgtctcg
    31201 gtgggcaaag atggcgtcaa gggtcacgcg caaggcgaag tgtcagcgaa ggctgacgtc
    31261 aaggccgggc ccgtcgatgt ccacgcggat gcttcagcca aaggtcgcgt cgatgaaaaa
    31321 ggtgcgacgg gttccgtcga cgccaacgtc accgcgaaga cccaggccaa agtcggcccg
    31381 atggatgtca aagccgaagc tggcgccaaa gttcacgccg atgaaaaagg cagccacgcc
    31441 gaagctggcg tcaaagctca ggcggaaggc aaagtcggtc cggcgaacgt caaagccgca
    31501 gctgacgccg gtgtatcggc ggacagcaaa gaaggcgtca aagcccacgc gggggccgaa
    31561 gcgcacgccg atgccaaggt cggaaatacg accgtgagcg gcgacgccaa agccgaagtg
    31621 cacgccgaca agacgggcgt ccacagctct gcggatgcgc acgtcggggc caagaccgnn
    31681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga aggcaagcag ggtccagtca
    31801 actggaatgc tgatgcgggc gcgggcgctt cgaccggtcc tgatggcgcg aaggctcatg
    31861 ccgaagtcgg ggcgcacgcc gatgccaagg tcggaaatac gaccgtgagc ggcgacgcca
    31921 aagcggaagc acatgccgac aagacgggcg tccacagctc tgcggatgcg cacgtcgggg
    31981 ccaagaccga acagaagatc ggtggcacca ccgtcacggc ggaagccggt gctggggttc
    32041 atgccgatga aaagggcacc accgccagcg tcgaagcaaa ggcccatgca gaaggcaagc
    32101 agggtccagt caactggaat gctgatgcgg gcgcgggcgc ttcgacgggc cctgacgggg
    32161 ccaaagctca tgccgaagtc ggggctcatg cggacggcaa agtcggcaac acttccttca
    32221 gcggcgacgc aaaagccggt gtcgttgccg atcagaaagg cgttcaggcc ggcgtcgaaa
    32281 ccaaggcaca cgccgaaggc aagcagggtc cagtcaactg gaatgctgat gcgggcgcgg
    32341 gcgcttcgac gggccctgac ggggccaaag ctcatgccga agtcggggct catgcggacg
    32401 gcaaagtcgg caacacttcc ttcagcggcg acgcaaaagc cggtgtcgtt gccgatcaga
    32461 aaggcgttca ggccggtgtc gatgcgaagg cacacgccga aggcaagcag ggtccagtgg
    32521 cttgggcgac cgatgctgcg gcgggtgcgt ccgtcggacc gggtggaccg cgcgcatatg
    32581 gcgatctcca cgcgggtgcc accgaaaaag tcggtccggt gacgatccac gaagacatca
    32641 acgcccacgc gagcgtcgac aaaaagggcg tccatgcacg cgccgatggc gacgcatccg
    32701 ccgaagctgg cggcaaaaag gtcggtgctc acggcgaagt gagcaacgag tccgacgcca
    32761 aaggcaacgt ctacaagaac ttctagccgc cgagcggcgc accaatcagg cgccgcagct
    32821 gaatcaccgc cagcgacaga tcaaagcgcg cctgcgcaag tcccaccgcc gccatgtcca
    32881 tctgatcctg cgacgtcaaa aggtcgagct gcgtcgccgt tcccgcctcg tactggagcc
    32941 gcaccaaatt cagcgtctct tgcgccattt gcagcgcccg ctgcgccgtc acaagaccct
    33001 gccgtttggt caccacggtg cgccgcgcgt tgatcacttc gtcggaaatg ctgtcgcgga
    33061 gctgttcgat ccgcagctcg gcatcacgct tttgggcatc gaactggtgg cgctgcgcat
    33121 cgcggacaaa gccgtcgaaa atctgccaat cgagctgggc tccgacggca aggctggcga
    33181 ctcgaccaga aaagcccgtc gcgtttgtaa aacggacgtt gccaaacagc gacaagttcg
    33241 gggaataacg cagccacgac gatgtgacct gcgacgacgc cgcctgcatc gacaaatcaa
    33301 ggctgagaag ctcgggccgg cccttgagcg cctgatcaac cagatcggtt tcggccccaa
    33361 gctcggtgct gggctcgggc ggcggcgtca cctggaagct gcccggctga agcttcagca
    33421 tcgtcgccag catccgatag gccgacgcac ggctgtcctg cgattcgagc agacgctgtt
    33481 tggcctggat gagcgccagt tccgcgcggg tgacttccac cttgttgacg acgccggctt
    33541 gcagcctgac tttggcgttg tcgacggtct tctgcgccac cgcgatcgta ttcgatcggg
    33601 cagcgaccag ttcttccgcc ccggcggcag caaaaaacgc tgtcgcgacc gattgcagca
    33661 cctgggccgt cgtcacatcg agctgtcgtt cctgcgcccg aaagcccagc ttggcccctt
    33721 gcagcgccgg atacgcggca ggaaccagca gcggcacgtt ggcgatgatc gtcgcatcaa
    33781 gctgcaggcg cggctggatc acgatctgcg gattggcact ggtcagcgtc gagtcaatgt
    33841 cggcgacgct cggcggtgtt ttgccaagct gcgccgctgg attgatgatt tgctggcaca
    33901 gtccatcgac ggcctgacgg tttgcgcccg tcgcactgtt gcagtagtcc ttgagcgcgt
    33961 tggtcagcga ccccgactgc aacatgccgg tcttggccag cgccaccgac gcatcagaaa
    34021 tcgccgcgcc ttgcagcaag ctcgaaaaga cctgctggcg ctgatcaaac ggcaccgaca
    34081 cttccggttc gttcaccgtc aaacgggctt gcgcgttgag cgtcggcagc agcgccgcca
    34141 tcgctcgctc gacatcggca tgagcgcctt tgaggcgggc gcgcgccgcg gccagatcgc
    34201 ggttttggct ttggccgatt tttagcgcat cgtcaatcgt cagcgtcttt gtcgcgctcg
    34261 tcggggcact gctctgactt ggctgcgcga tggcaagcga cggtgacgca agagtgagtg
    34321 ggcacaaggc gaacgcaaag aggcgcgcgc tcaggtacat ggtgctttcg gtcctttctt
    34381 gaaatggcaa tcgcagctac ggaaccagct tgaggtggct tggccgcggc ttctttccgg
    34441 gcttgggcgg tggcggcggt ggctcgggcg gtggcggctg ggcgtccatg tccttgggca
    34501 cgtcttcttc ccagaccaga ccgcgctttt cgccatcgtg catcgcaaac accgcggacc
    34561 acggaatcaa cgtcgcatag ggcgtccgct gaaacgacag ggtagctttg atgccttcgt
    34621 cgtcaattgt aaggtcatga atcggcatcg gcatgttata gccatattga agaacgagcc
    34681 gaggctgact gcgcagatga tcggggagga tgacgccctc cttgcgaggg tcgagcacaa
    34741 cgtagaccca agcatctttg aggagttcga gcatcacgtc tttcttttgc gggagcatga
    34801 cgattctttc cgtgactgga atcgaacgta ccgtagcacg gcctctaaaa ttggcacgtc
    34861 tgtctagcaa agccgcatcc ttgcccaagc tgttcccgcc atgacacacg ccacaacgca
    34921 cgaaaaagac gaaacccact ggctgtaccg actgaccgct gatgaatggc tgcgggctgc
    34981 tgccaccgag cttcgccaca gtcggcaagc ctttgaaaac aagtcccagc gcgcagccgt
    35041 cgcacatgcc cgtcgagccg gcggaatggc gataaacgcg ctcttgtggc tacgttccga
    35101 cgattcctac ggacgcagct acatggacca cttgggtcgg ctcgccaaag atccggcggt
    35161 gccgtcgtcg ctccaggtgg acgcgcaagc gctgctggcg atgccgatgc aagccgcttt
    35221 ggtgcagctt gggccaaagg gtgatcccaa acaggcagaa ccagcggccc gcatcctcga
    35281 atgggcgcag aagctggcgc agagccatca gacgggaaat tgatcgcgcc gggcgcttat
    35341 tcaccccgga agttcggcgg tcgtttttcg gaaaacgcga gcacaccttc ggcagcatcg
    35401 gcagtcttgc taagcaggct ctggctgtgg gcttcccatt cgagcatcgt cgccaagtcc
    35461 gaagtcgccg cccgcccaag cgcacgcctg gtcatcgcct gtgccagctt cggtccgcgt
    35521 gccaattttt cacacagcgc aagcgccgcc ggttccagct ctgcgtcggg atgcacttca
    35581 tgaaccaggc cgcacgcccg cgcatcttgg gcacccagca ccgccgccgt catcagcatg
    35641 tgcatcgctt ttgccgtccc caccgcctgc ggcagcagcc acgacagacc aaagtccgcc
    35701 gacagaccga cgcgatgaaa aacggcgcca aacgtcgcgc tctcgctggc cagacgcaga
    35761 tcacacgaca gcgcgagcga cagcccagcg cccatgcaga cgcccggaat cgccgccacc
    35821 accggcacat caagctgctg aagcgccagg atcactttgc tgtcgcgcag aagctgctct
    35881 cgccgcgccg caatcggcat cgccagccgc tgcggcatgg tttttaggtc gccccccgaa
    35941 gaaaatgcgc cgcctgagcc actaacgcaa acgacatgca cttccggttt gcgcgcgatg
    36001 tcatccacat gcaaaagaag ctgttgccag tccgcgggac gcagcgcgtt tttggcttcg
    36061 ggacggctca gcgtaagacg ggcaatacgc ccagcttgct catacgaaag aacgaccgac
    36121 atggagtggt ttatatcgcg attcccgtgc agcttccgca aagctgtggc tactattgtt
    36181 accttaggca ccgattcttt tgacctgtga gcgagatgca caacatcctt ctttcggcac
    36241 tgcacctgga ctttgcggcg cttctcgatc cggtggcgca agctgcgtcg gcagccgcaa
    36301 gcaagccagg aacgtatgat tcgattcggg actggtacaa atccgccgac cgaatcacgc
    36361 tgctgacgct gggcgcgatg attctttgtt cgctggccgc cgtcactgtc gcaatcgagc
    36421 gcacgctgtc tctgtggaac attgctgatt cgtcgcggcg gctcgctgag caagtgcgcc
    36481 gggcggtcta tcgcggtgcg ctcgccgatg cccgcaccgc gtgtcagcgc agcaagtcac
    36541 cgctcgccga tgtgatgctg gtcggtttcg agcgaatcgg tcgcagcagc aacgaagcgc
    36601 tgttcgccgc cgtcgagcgt gaacgtcagc gaatggtcct gtcgctgcgc ggcccgctgt
    36661 gggtgctcgg tacgattggc gcgacgactc cctttctggg tctgtttggc accgtcgtcg
    36721 gcatcatggg cgcgttccgc aaaatcggcg aaagcaacca agccggcatc caagtcgtcg
    36781 gtccctacat cgccgaagcg ctgatcgcga cggcagttgg catcggtatc gctgtcgttg
    36841 cgctgctcgt gtttaactat tttcagtcgc ggcttgcgcg caccagcatt gaactgcgtc
    36901 ttgcgatcga ggaatttctc gaagagctga ccgaggcgca cggacgcgat cctgatgcgg
    36961 gcaatcccaa gtcagacgac aaatctgacg ggaaagacaa acacgcaaag accgccagcg
    37021 aggaggcggc ctaacatggc catcggcaaa gttcccggct cggctggcga aggcgatctc
    37081 gatgaggaag gcatcttcgc cgagatcaac atcacgccgc tgaccgacat tttcctcgtg
    37141 cttttgatca tcttcatggt cacatcgtcg gtgatcgccg atgcgggggc gcggtcggga
    37201 ctgcgggtga acctgcccaa aggcgcaacc aaagaagtca gccccggcag caaagacgtc
    37261 acggtggcca tcggccaaga cggaaagatg atggtcgacg gcaagctggt cgatggaacg
    37321 gaacttgcgc gcctgtttgc cgaagccaaa acccgcgatc ctgcgaccca gatcatcgtc
    37381 cacgccgacg aatccacagc ccacggtcgc gtcgtttcg
//
LOCUS       DAORGN010000021        37078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_404_length_37078_cov_5.688221, whole genome shotgun sequence.
ACCESSION   DAORGN010000021 DAORGN010000000
VERSION     DAORGN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 37078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37078
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_37078_cov_5.688221"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKL17_04185"
     CDS             complement(<1..789)
                     /locus_tag="PKL17_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNK43957.1"
                     /translation="MTARRIVRCALTQTKNAYAAMPPTVEALPSLRGHLDAVRAANVD
                     HNISLIARASAHGARVICLGELCTAPYFALYTDPMWLAFAEDAHTGPSVKAFCEAAQK
                     HSIIVIAPLFETDSQSGKRFNTAVVIDEQGSVIGKYRKTHIPCGQNDRGSFHETFYYG
                     PSDGNLGHYPANIARNPYFPVFRTSVGNIGVAICYDRHFEGVVHSLKEGGAELVFCPA
                     ITFGKKSQKVWHQEFFVDAVRHGVFIGGSNRLGQEPPWTIEYFGE"
     gene            complement(786..2120)
                     /locus_tag="PKL17_04190"
     CDS             complement(786..2120)
                     /locus_tag="PKL17_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNK43958.1"
                     /translation="MISLRCAGCGFEVPLTEGMPPFVCPKHSDGGFHVLRPYLPLPSV
                     GGTPAFAHPFLRFADRMLAYHRCREYGLAHAELYHSTVEKLTEQVTHLTGKSFSHTPL
                     YRSDALSDWFGLSERGGVYVKDETQNPGGSHKGRHLFGIMVELLVGEALSLAGSGTHR
                     PPLAIASCGNAALAAAVVAKAAHWPLQVFIPDDAEASVVSSLRELGATITVCSRSAGE
                     TGDPCYRRFVEAVKSGAIPFCCQGPDNGLTLDGGQTLMAEALSDMVSLETIPARIFVQ
                     VGGGALAASCALAVETALCTGLLSHRPRFFTVQTESVSPLSRALAKLRAEYGPQGRIL
                     SDAEWRQIAAHKAKYMWPWESVPHSTAHGILDDETYDWLPCVKEIWKSNGDALTVPES
                     AILAAHDLAKRTTGIAVSATGSAGLAGLLTEHKHSPLHPDENVLILLTGVAR"
     gene            complement(2117..3592)
                     /locus_tag="PKL17_04195"
     CDS             complement(2117..3592)
                     /locus_tag="PKL17_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNK43959.1"
                     /translation="MSSATLPTDSQALHNAMQRLRKSKVLLPQLSQLADPGLIPAEIQ
                     TALRDVDPDAPHPLNLWRVHWYNDASRRGIQSVPAHLVLHKELTGVDAKIVVLLGSRF
                     PMIAAHKVLAAYGCLVPRLVAGEFDPTTQRALWPSTGNYCRGGVAISRILGCRGVAIL
                     PAGMSAERFNWLSRWVADDSDIVRTPGTESNVKEIYDKCNELRRDPQNVILNQFAEYG
                     NYLVHYHCTGRAAEAAVLANSGLQVAAFVSATGSAGTIGAGDYLKEKHGARIVAVEAA
                     ECPTLLANGFGEHNIQGIGDKHVPLIHNVMNTDVVVAISDHSTDGLHLLFNHPEGQRY
                     LRDRRKLSPELVASLSGLGFSGICNILAAIKTARYLRLPETEAVVTVATDSAAMYGSE
                     IPKNEARYFPTGFDQLAAAETFGQHLLGVRTDLMQELTLRDRERIFNLGYFTWVEQQG
                     IALKDFEARRHQSFWRALRQNVPRWDEAIAAMNARTQDGVA"
     gene            complement(3638..4846)
                     /locus_tag="PKL17_04200"
     CDS             complement(3638..4846)
                     /locus_tag="PKL17_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK43960.1"
                     /translation="MSEWLLRQSQYGDVRICDDRITEVSSHSVIGDAQIIDCEGCALF
                     PGQVNAHTHLYSGLAPLGMPAPTVAPQNFVQILERIWWRLDRALDADSLRAAARYYIS
                     SALLMGTTTLVDHHESPALIAGSLDILADACAELGCRALLTYGATERNFGRREGDDGL
                     SECRRFIVTNQRPFVRGLVGLHASFTVSDETILAAGQLCRELGTVMHVHVAEDLADVE
                     DAKKRGYRDPLDRLLRLAALPPGSIVAHGVHLDADSVRRCQDEGLWLVQNPRSNFGNR
                     VGYPHALSCSARVALGTDGYPANMAEEEAALRTRAKEFGEADSVISRRAQSGHILVAE
                     RFALPAFAIAVGSPADLVVRDPSGQVRHVFCAGKLVVRDGTLVTADRQEIEHDAQQKA
                     FHLWNRMTAL"
     gene            complement(4855..6630)
                     /locus_tag="PKL17_04205"
     CDS             complement(4855..6630)
                     /locus_tag="PKL17_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNK43961.1"
                     /translation="MPNLLVINADGPETRVALVENGQVVELYIERRRERGIVGNIYKG
                     KVLRVLPGMQAAFVDIGTDKAAFLYVADVRGAPEDIKSLFVDEDDEGSRKEDDKSRDA
                     RIEDLLRPGQEVIVQVAKAPIGTKGSRSTSYISLPGRNLVFMPTVDHVGISRRIGSEK
                     ERKRLRSIVDSMRPPGTGFIVRTVAENVQEQELRADMEFLIKLWNSIVKKSEAAKAPA
                     LLYHDLDLLLRTVRDLFTSDVEKLIIDSKPEYDRLIKFTSTFMPEYVSKIDLYEQSEP
                     IFDAYGIEMELDRALERKVQLKSGGTLIIDQGEALTAIDVNTGKFVGKRNLEETITKT
                     NLEACKEVADQLRLRNIGGIIIIDFIDMDREANQEKVMRAFLDSVKPDRARTNVTKIS
                     ELGLVEMTRKRTRESLGRMLTEQCVYCDGKGYTKSKTTIAYEVLRHIRRDGHAFADDT
                     IVVHTHPEIAELLASADNEYIEALEKRLQKKIIIKPRERFDLVEGHIEQFHIRGASTD
                     LKKEKPQDAGRAKDRGRIPDRTGNPSAPVAAPIAQVAASPVSLAVPVSPVLMPAAPGP
                     IVIPASSSVAEVPVANDPATGDAKK"
     gene            complement(7039..7869)
                     /locus_tag="PKL17_04210"
     CDS             complement(7039..7869)
                     /locus_tag="PKL17_04210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNK43962.1"
                     /translation="MSDQTVLRGLLHDRVAVVTGAGRGIGRVCATTLVSAGASVIIND
                     LDAEPAAEAVAECHAIRPGSAVASIGSVTDPKYTDELMKAAVDRFGKLDILVNNAGLS
                     RDKMCHLMPDEWWDLVIDVNLTGTFNCIRSAAPHIREVAKRELEELGQPRYNRKIVNF
                     FSTAAIRGNPGQINYVAAKMGNVGITRVVAQEWARFHVNVNAVAPGFTETRMTAAKKP
                     GDVLGVPEEQRQATLQKIPMGRFGQPQDIANAVLFFSSPLSDYVTGQTINVSGGMQIP
                     "
     gene            complement(7936..8529)
                     /locus_tag="PKL17_04215"
     CDS             complement(7936..8529)
                     /locus_tag="PKL17_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK43963.1"
                     /translation="MRLIVLTGGPGAGKTAVLDIVSRMLNHHIAVLPEAASLLFAGGF
                     PRRDSPAARRAAQRAIFHVQHELEALALEEGKVHCAICDRGTLDGLAYWPGPAQDFFS
                     QLGTNKADELRRYSTVIHLRTPQVGYNHQNPLRIESAEEAHAIDERIAQAWADHPNRV
                     FIENAPDFMSKAHAALDAIVAALKESCHAELSIPSLP"
     gene            8689..9003
                     /locus_tag="PKL17_04220"
     CDS             8689..9003
                     /locus_tag="PKL17_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43964.1"
                     /translation="MLHKPHRPRFVRLSAEHARAVLGGGADLEPWMVISNGRFVAKQR
                     ITMIGPRGRIDGVAVVGPLVESTQVSLAAHDEEKLGLADDPGRGVLLVGPGGEAKVVA
                     SE"
     gene            9006..9581
                     /locus_tag="PKL17_04225"
     CDS             9006..9581
                     /locus_tag="PKL17_04225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNK43965.1"
                     /translation="MSIRINRVYTRTGDDGGTHLVGGHRVSKDDLRIECYGTVDELNA
                     TIGLARLHANTPATADLAAILERVQSELFNLGSQLATRAEDLRPTQPVILARHIDRLE
                     QEIDRCNEQLPALTSFVLPAGGALTAHLHLCRTVCRRAERLAVSLMHREPLSPEVVQY
                     LNRLSDALFVFARHAARVTGEAEVLWQPEKT"
     gene            9838..10542
                     /locus_tag="PKL17_04230"
     CDS             9838..10542
                     /locus_tag="PKL17_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43966.1"
                     /translation="MGTLLRTLALGIAFAATVSGCGQGNDDTDIDTLPVVTEDDKADT
                     TGSPLIWVRPSSFPIYCIGYPCATKQVAEVNGGEGRLIFKYDWRALKLTATEQTTAEA
                     NHFNMLLRGRYNTVKVSGQPMTVLQISRASVKVSSSSDDNAEKDSFYLTKEAPCSKQP
                     CPAVQAEMINKTAAPEVWATVDLSRLGLSTTQQQSLLTEMQDKTGQVYVSQATTPTGS
                     KQISQAFRSIKSPALK"
     gene            10681..10872
                     /locus_tag="PKL17_04235"
     CDS             10681..10872
                     /locus_tag="PKL17_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43967.1"
                     /translation="MSTLREKDEKVAQKKGLMAGAAVVASGAAVVVGAPVLGVLGLLP
                     AAYLTYDWFMFRAKRGMRF"
     gene            10894..12828
                     /locus_tag="PKL17_04240"
     CDS             10894..12828
                     /locus_tag="PKL17_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK43968.1"
                     /translation="MSLCPTCKSNFSGGEQFCPRDGTPLRPSLPDDPLSGRVLSGRYR
                     LLELIGRGGMGAVYRAHHILMDKPVAVKVLRGELSSDTEAVARFHREARSASRLDHEC
                     IIRVTDFGQTDDGLLFLVMELLDGENLAQVIRRAALPWRRAATIARDIAQGLAHAHEQ
                     GVIHRDLKPENVVILRRGKGRQLVKVLDFGLAKLMHEAGPSGSAPDEEPGRALTKTGV
                     VFGTPEYMSPEQAEGHKLGPHTDLYALGVVMYQMVTGKLPFTAPTFLALIAKTVHEPP
                     PPPSNVAPDAKLPPGLEQLILRCLEKSPDDRPNSAEEIADELDALLAQNLDDVGDDRA
                     TPASGAAKPTASASQPVVLAPAAVPPGGVAKTVQRVNPARPGGVTPSTAAVASAFEAL
                     AKRGQPVSGPPKALPVPAGPEDDATAAAASDAGVVDDSAPPAVTAPAVSSQSPSPEAT
                     TVADPEPARAAPRKEIQEAPSTSSGPVAVPRRPYWLVGALLLSGMVGAGVYFGPKLLG
                     KNSQPVSVDSDEVRQAKLLLATDKSPAGIEAAIRLLLLARQKQETPELHRLLSGAYEA
                     QGNRLRALGHMYLAVQRSQTMSERSVSQLALAQLLSRLGHSQEACQTTQRLLREKPDS
                     SGEVKVHAQALLSALRCDAK"
     gene            complement(12886..14106)
                     /locus_tag="PKL17_04245"
     CDS             complement(12886..14106)
                     /locus_tag="PKL17_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43969.1"
                     /translation="MPLVTEKQPSHRVPVRLTAGSSWGWLCALLLFVLLPKTAGADGE
                     EPLLWLAIQRGGALDGVGSEQVREQLAGRKELRVVESTLTDADRRCRTAACLSALARQ
                     HQAYVVLSGDIFQLGSKKSQRVVMHLYDGRHRQVFDVENLCNDCDETKLGLLVQSTVS
                     EILSRYHKGQDADAQLNELLEGTAKNGPPAIPTPASDPVMVPPLVPVTPPPQLAKTAA
                     PVGTDSTASSRPQLAPMPVAKPAESAKVQLATTPVASPQAPSPNVPPPLPPQGNTPVL
                     AGSYVPQLPSTAQTAPSAPPLYPTQAPLWTQTPQGTPPQQRKGLSTKRKALAAVFGTL
                     GFAALGGTIAAHYFDKRLDSTLAQNPGGLPCSSQANAGQNCVLTTVGLWAPGYAVSGL
                     LIGGMILSLAIPEK"
     gene            14320..16026
                     /locus_tag="PKL17_04250"
     CDS             14320..16026
                     /locus_tag="PKL17_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43970.1"
                     /translation="MDASESCRGDSLGLAAWVRAAFLQVGFWLLAAGIGFGAATVTSC
                     VVPDPAYCGKDEECAGRPESSDGTMLVCHPTKHMCIPNKPGQCFKDTDCPSTKASHCD
                     TSTNQCVSCRVGDPTDTSCASLGLGSMTKCLDTPSGPQCIECQQHLDCPSERPICDGG
                     SCRSCREHKDCEGTLNCEGGVTCTDSMVCIRDGDLPEGRAGSCAWNGPGSTGRVVYAH
                     NGNAACSDTDPSYGFRFSEPVCNLARAFAIARDTGRRYIRILGSNYDPLNATILFGTY
                     SFIGAPGKNYPTLATMKGRALLFDIRDTARVTIDQLDMTEQNVDAGAIACSGPGADRI
                     PGLTIQRSILRGSTPPSFPNPTSSAINVNDCAVRIEQSIIGVTKLADAMMASTATAHA
                     TAIRISDIWQSTKASYVLENNLIAGNTAIAVDLLNTDRLQQKLVMRFNTVAYNGRSNN
                     GKAGGIRCATGPSVKPAITDNIFFGNSTVGASQIQFGEGCSFGDTVVGSADSCAEAGL
                     RKNNPEFFDDFSLKDGPANRNCCIDQVSPPSGDTFPTRDLLLSSRPSGAKYDIGCYEL
                     PQ"
     gene            16058..17032
                     /locus_tag="PKL17_04255"
     CDS             16058..17032
                     /locus_tag="PKL17_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNK43971.1"
                     /translation="MLLNALKKTAGRPFDLCGLGECSVDLVYRVPLRLDKPLPDKVSA
                     KAHEVLGGGQIATACAAAARLGRKVRFVGAVGMDGAGQALLAELASDRVDTSLVRRCL
                     GVQTRTALILCGDDGERSVIEWRDQGLTLPPGLPSAEQLSTARVLHVDLCFPDATLAA
                     VVAAKAHGLIVSVDLDRPLPRVDDLLMLTDLCVVSTHFPSRLTGIDDPETAALALSDR
                     TKGRVVVTQGEHGCWLVCDGAIVRLPAFAPPRLVDTTACGDTFHAALICGLLDAADGK
                     TGSRGDDESALRDAIRFASAAAALKCGDLGRRGCPQRAQVDQFLQTAH"
     gene            17114..19438
                     /gene="eutB"
                     /locus_tag="PKL17_04260"
     CDS             17114..19438
                     /gene="eutB"
                     /locus_tag="PKL17_04260"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNK43972.1"
                     /translation="MKSASWSSLRRRSFLAAMLGGAAAAALPGCGSSPTPAPGVSIPS
                     VYSEESVFDYLQRTVGSFEQTRYRQIIGAASDYKQGDEAIGVAAADETSRQNARTLLS
                     NTKLGDLAAHPLVDDRQGQLIAQTTDAAMQKAMSSWTMGFLKSYLLAATEDSIRQVKN
                     GLTSDVIGCLVKLLTNSQLVALSQKIWNPLPGSKVGSKGYLGARIQPNSPTDNLDDIK
                     WQIFDGFAYAVGDVVLGCNPVSSDPESVASIERMMQDLRETFGLTDILPHCVLSHIDI
                     QSEVETKYPGQTGIWFQSIAGTDGANGTFDVTVEKMLRYADQRTGKYGLYFETGQGAD
                     FTNGQDFGVDMVVLESRKYGFARALKQRVALAQEKAGQTVAPWVHFNDVAGFIGPEVF
                     RFRDQLVRCCLEDILMGKLHGMTIGLDVCSTLHMDVSLDDLDYCLDQIMPANPAYLMG
                     LPTKNDPMLGYLTTAYQDHVRIRDRFGYKVDDRMWAFYKQLGVIDQAGQPTDKFGDPR
                     WVYLQFLRRKGDKRTDAVIYAEADQKMKEVRERGVWLAQGYGQKPWNLDPKLDAEIHA
                     LYEDSRKAIWTEWTPAFVASVPMVEQLHTKSSDRKDYVWHPPTGEHLDDDSASAVAAM
                     RLRHRDQYNVQIVISDGLCAPALSDPNHLAPYLESLRSELQKQGYKPSPDNVVVRGGR
                     VRAGYHLGELLYGGLSNAAATKAIIHLIGERPGSGHHAFSSYITAPPVSTWSQPGKVD
                     HNITKVVSGIADTAYDPRSAGIETVTILQRIAPP"
     gene            19586..20353
                     /locus_tag="PKL17_04265"
     CDS             19586..20353
                     /locus_tag="PKL17_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43973.1"
                     /translation="MAKSVLGSVLRLASVTVLLSALLSGCTSGGGSGPMNPAAKADMS
                     VAPECATMKCENPSAICCGGEPCIDVLSNTQHCGGCGKTCRSREACNNGNCVCAGGGR
                     TSSCKVTEACCSDGCHDILSDTKNCGGCGVSCKEGEVCDSGQCKCGPSGIACKTGQIC
                     CGSVCSDRQNDAMNCGMCGKACKPGKACKNGLCEGECVGCTAIETCCDGTCVNLLNNQ
                     MNCGMCGNACKVVLGIPLPCLIGICSFSMPDMGARDM"
     gene            20370..21110
                     /locus_tag="PKL17_04270"
     CDS             20370..21110
                     /locus_tag="PKL17_04270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNK43974.1"
                     /translation="MSISLHVNVDHVATLRQARGVSYPDPVWAASLCELAGADGITVH
                     LREDRRHIQDRDVELLRKTVRGVLNLEMAATPEMVDKAIAIRPDIVTLVPERREERTT
                     EGGLDVAGGARLAVQEAVAALHEARIPVSLFIAPDLTQVRAAAQLKVPRIELHTGQYC
                     EETLRPGHAAQAAKELERLAQAAEDAVRLGIHVAAGHGLDYPNVKPVVRIAGIEELNI
                     GHAIVARAVLSGIEQAVRDMIALIRQNQ"
     gene            21107..21514
                     /locus_tag="PKL17_04275"
     CDS             21107..21514
                     /locus_tag="PKL17_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNK43975.1"
                     /translation="MTTAEKIAGFLSAPLFAVAGASNDPDKFGYRCFVALRRSGRAVH
                     PLNPRAAEIAGSPAYPALRDVPGQVVALSVVTPPQVTEQIVADAILAGVRYIWMQPGA
                     ESRTAIAKAEAAGLVVIAGGPCVLVELAKQGSV"
     gene            complement(21511..23115)
                     /locus_tag="PKL17_04280"
     CDS             complement(21511..23115)
                     /locus_tag="PKL17_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNK43976.1"
                     /translation="MLSLPRTVRVFLVALLALSFVGCPAPRRDDDPPRPSPARPVATG
                     VPSCQVAWAQLPAGDRGYQPAQDSEQTPLGNQGYRRYLERTARPKCEKEWTVLLYMAA
                     DTKDLQKPALQTLRAIERAGRLAADGDADLIVQIDLQKQPGIRRLHVLGLGDSPADSD
                     DPSTLRSPIVEYTSDENQPPQDSLRSFLAWGIDHYPSRRYMVIVWGHGLGWRPRAGKD
                     QRAIYDPSRPLGGIAPDESERTVFDIPSLRDALRSASEEKLSGRPFDLLATDACLMQT
                     VEVSTELADTARYFVATETKVDYDGLPYGAILESLVSPDIAPESVPHSSRCGRDDRSC
                     VLALKLPAISMSQTGTAPGATRGPNFLLSVVELSQLRTGLSPSLQSLGQALAAYLAED
                     PTRSVAIQQLLTVKTGVQFGASRTLPDFPGGTRDLGVFLLNLRTVVRKEASRSESPSC
                     RAVLSAIDQSESALASSVLSLAAGNRYEEERFRGLAGISAWLPSTPEEWTERRPFFAP
                     SLFYRRSDTGLLSGWESFLSALYAGR"
     gene            complement(23134..24612)
                     /locus_tag="PKL17_04285"
     CDS             complement(23134..24612)
                     /locus_tag="PKL17_04285"
                     /inference="COORDINATES: protein motif:HMM:NF027993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43977.1"
                     /translation="MDDVALQESLDAFAANPKAATATPPPKYDPNGRPVTRSTTFFDA
                     SDLANGHYVLHRDSVRKTFHTVIDGVVISLEDATPGKAPIEAADRPENLVDRLVYRRL
                     AEMDKAKLLKARLSQNPWSSDYWALYQGCLGKRYADPKFPGSLKWEENHAYVKQHPVS
                     AVLSSGGAGAIDLLSPAEKYDLLAGDPTGTLATAMWDEGRSYFDSSGSVETWMGICHG
                     WAPASYMMPRPSKTVTVLAADGKTQVRFNPTDIKGLASLLWANANTVTRFIGGRSNDK
                     DPAVDENGRPLSPQVFDTNPGTWHLCVVNQIGVAKRGFVMDATYDYEVWNQPVLGYSY
                     RYFNPQTMRLATKLSTATISRAKFTRDPFRKYRSAEAASFVGIIMEVSYVVEKLAAPC
                     STDGPGDDAVRTVVYKYDLELDASGRIIGGEWYTGKCHPDFLWTPPGTARAIGPAEQF
                     ATGGWTPGQPLPESWRKAAVRSAESGIPLAKIIESLISFANT"
     gene            complement(24674..26158)
                     /locus_tag="PKL17_04290"
     CDS             complement(24674..26158)
                     /locus_tag="PKL17_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43978.1"
                     /translation="MSGRTALSAVVVCLTVAVVGLWASRRRNRSSLSGVSGAMGPAAG
                     TSHADLSSSGMVPTLRLPQPTEKLPQLLLALEDARARHDISEECDVLLDLARAYSDAA
                     DYPCAVDNLSVCLRQSTQHRDEWFAGEAHLEAAYVHLRAKQLDRAVQACEAALRLAEQ
                     RKSSTLTVQALDRLTDVWTLSGRLPEAIAAAQKRLHATAESPASRVPVLLRLGELQLS
                     SRRYSESSESFAAALAAAERTGQERDESRVIARMARAALLQGQPQRAVEQISAKLEQV
                     VSWSELFDSARVLSVLGEAYLELGMPGKAVVALVRHRLLLRHHNDDRVRLLALLSIAH
                     RQAGQHADAVACLRECDGLTSKLGPATLPTLVTLSCAFVECGEAQASMFTAQLALDQA
                     RNRETADGESWALWALARAQLKLGQTQRATVGLSQAIEKLSTPKDKRIESLVHDALAE
                     ALDKLGQTAAAAASRSIRNQYLQRIGRSPALASTGSAASAFPRE"
     gene            complement(26219..26545)
                     /locus_tag="PKL17_04295"
     CDS             complement(26219..26545)
                     /locus_tag="PKL17_04295"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK43979.1"
                     /translation="MAKEAMIPRAHRTLVNAEFEAVDQHLSEYVVNVSRTGAFIRSKD
                     PLPVGTRVNVRFSLLLDEIEIVEGVAEVVRVQSSPPGMGVVFRRLSEASARLLERLEQ
                     SGAAAR"
     gene            26683..27300
                     /locus_tag="PKL17_04300"
     CDS             26683..27300
                     /locus_tag="PKL17_04300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNK43980.1"
                     /translation="MNPKSADHDADGTDLFAFQMPSPLGDLCGVASPLGLCQLTFAPL
                     AEALSGYPSPLRQQLFESAITVPDGGQAMPSHLPLRPLGGWLKTLARYLHGESLRLDI
                     PVDLRLARSPFHRAVYESLLQVGPGQTTSYGDLARIVGRPKAARAVGQAVGRNPVALV
                     IPCHRVLGRDGSLHGFAFGLEKKAALLRLEGVKSKMPQASLTLGS"
     gene            27426..27950
                     /locus_tag="PKL17_04305"
     CDS             27426..27950
                     /locus_tag="PKL17_04305"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK43981.1"
                     /translation="MSDSSQLVRSYADLLDLLRRDGVPHEANLETSEVVIPTQRGPLD
                     AVLLIRWQADAGVVQMIQPLTFEVPPERIPAMEAALCRLNPALVVAGFELAYERRRVG
                     FRTTLPLQPRGGLLPDEIQTYFRITVKTAADFLPTLSRIASGIGKPQNVLSDAQSDLA
                     GGNSGGGPGALGTY"
     gene            27996..28343
                     /locus_tag="PKL17_04310"
     CDS             27996..28343
                     /locus_tag="PKL17_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43982.1"
                     /translation="MSQPDVHQTFLAAIESAYQTLDSNFDELYSACHGAEEKSQLRSL
                     HAAARDAYWRGVATKLCDDNSVVRAVYEDLNRANQELRDALRDLQQLSAVILLATQAV
                     RLAASLVTLATVG"
     gene            28348..30873
                     /locus_tag="PKL17_04315"
     CDS             28348..30873
                     /locus_tag="PKL17_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK43983.1"
                     /translation="MRATLRGLILSLCVAEMTLPALPALAQSASIEQEWRILTDAQSA
                     YQRGNAAAERGDKDGAIREWDVATANFQQVMRMNPMRTDLYAPLADIHIRRGHPAVAY
                     ALLTQQMRGGVNDLAVRIQVVRALRAMNRRSKAIAQVEELRKDRPNDPDVGALHAELL
                     AEDGQSDQALMLLLLVLPKLPLEGRYPGLDGASMRRLHARLLLAKNRGAEAVGSLTEL
                     LKARPTDAEAQLLLGQALLGSGKHAEAVAALRRYLAQFPSDGRARAALGLALADSGQL
                     LAGIEELEHAGEVPEALSQLGLLYLRKVPPDSPAAVAALTKALQLQPNNLKVCVALAE
                     LRDQAGQGAQALTDIERCTEPLLGADGGLGPVDGTAQKAVLLRLDLQLKNNKFEPAMQ
                     TWRGVQARYPGLSSLQGKLAAALLRRGLSKLPTGSQPSNAGLADLQEAHKLAPSLASA
                     QALALGQIGAGQPQDALQTLQNYLKDGSTEPRFLGAYGRALRDAGQAQDALLVLQKAE
                     GLTSSAPSNQALKLALRQELALCYLQLLQPRLAQRYLDGNDELSQQIRAQSNLVSARI
                     LFSSATPGTMPSFPSVMFVTQAVLRGGPAVLPSQRAEAKLWQVVGLAGNGQIDVAVKL
                     IAEVASLFDVPTLELVMGRGGLPHLQARLALRIGDYYGGAQLAQQAMPSLSPDQVKAL
                     HVSITTADNNKAVDPVGKGDLERANGLLRAAVQQAQAGNSPHLLKVLYNLAVVQLQRG
                     RHDEAKSTLAKVDPVQLPEALVAQGALAEAVGDPRGALEQYRRYLSAGGAANPPGEQL
                     ASVRDWVDAMARFYEGAAPHAEPASDASPKPVKKPVAAPRRQP"
     gene            30870..31787
                     /locus_tag="PKL17_04320"
     CDS             30870..31787
                     /locus_tag="PKL17_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNK43984.1"
                     /translation="MKRWFRGLLAFAILFWSLTIQAAAPSEKVLAIYLPGVYFAQLER
                     KLDLGSELVAHLLKRLGDKYHLSPRVYVSAEAMDADAARIAMVLTESPYVAANLTKIL
                     PVAVAAAGNGTETRMALLATPTIRSLPDLKRGGLVFASSLEPAQSFLDNFVFEGELTL
                     SRDMMSPVRDVASALSLLSVQKAQAVLLYEDTIATVRTANLRTLYRSDQLPRPTLVLT
                     SRSTDPADVQKLREALTSFHGQVHPDIKAFRATTDQPYQKLRDRMQQKPRRNPQLLLI
                     EDDTSPLPTPRTNLPSPVQVPLRAFAPEG"
     gene            complement(31788..33209)
                     /locus_tag="PKL17_04325"
     CDS             complement(31788..33209)
                     /locus_tag="PKL17_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK43985.1"
                     /translation="MPASPRRVVIADDEPSLRKVLSSLLRREGYDVQEARDGEEALAL
                     LNSDVAVLITDLRMPKLDGMQLFRRAISEYPDLKVIIFTAYGRITESVEAVKLGAFDF
                     VEKGTDNEHVLQVVRKAVASYELDLRAPRKPQGQIPPQLGRYGLVGTAPALQKVFSLI
                     DKVADTPSTVLIIGESGTGKELVARALHEHSSRKNESFIRINCAAIPKTLMESELFGY
                     EKGAFTGAVHSKPGRFELADRGTLFLDEIGELAVEMQVKLLRVLQESEFERVGGIKTL
                     RVDVRVIAATNRDLVKEAAQGNFREELYYRLNVVPIQLPPLRERLQDIPLLVSHFVSR
                     FNERLRKTVSGLSDDAMAALLRYPFPGNIRELENIIERTVVLAEGPVIELRDLPPNVA
                     QTAGAQLPPPPRMSQPQTPSSRPPATSLKEIVRQETERVEREMIQRALSETQGNVTQA
                     ARHLGISRKSLQIKMKELKLREE"
     gene            complement(33242..34159)
                     /locus_tag="PKL17_04330"
     CDS             complement(33242..34159)
                     /locus_tag="PKL17_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNK43986.1"
                     /translation="MTASLFTDEPASPPERTTRVLIVGAGPVGQTFERALQHAGYPVR
                     TWSRSHGQLFPEGTAQQCADIVILAVRDDAVSQAAEYITSHGAAGASSVVLHCAGSRP
                     PSQVLASIIGKVRGIGLLHPLRSFVHRDKSDDSDGSGPHSEQELRGTVMAIAGDGAGM
                     LAGQELCAALGGTPLLLSEDRLPIYHAAAVMAAGHVATLLDVASQLLARVGLPRQVGQ
                     RALTDLTQSVLRNIEEVGLPQALTGPIARGDAMTVAAHLDAISRVSCQVADVYRIIAE
                     SSLDVAYRKGAADDSTLAAVGDVLQSRRV"
     gene            complement(34156..34755)
                     /gene="nadD"
                     /locus_tag="PKL17_04335"
     CDS             complement(34156..34755)
                     /gene="nadD"
                     /locus_tag="PKL17_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNK43987.1"
                     /translation="MRVALFGGSFDPPHVGHQLAALYVLETFPVDELWLVPVFRHVFD
                     KRLTPFRHRLAMCQLLADSLGPRVHVSTIEEELGGPSYTLHMVRRLMQKHPSVEFSMV
                     IGSDLLKERERWFGFPELAKLLPFLVLHRSGSDSDAVVPVSSGDISHHEVLRLPEVSS
                     TGCRAALSKGGRPTGWVCRRVLDYIEQHALYQASEEERA"
     gene            complement(34871..36085)
                     /locus_tag="PKL17_04340"
     CDS             complement(34871..36085)
                     /locus_tag="PKL17_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK43988.1"
                     /translation="MPALAPYHRRLFLFLSVASFFEGFDYYALAQLLPELRTAFSLTV
                     PEGTSLASFINIGMVLSFFLVRQGDRLGRHRLLAWTITGYMVFTALSALSQNVGQFAV
                     CQMLARLFQSAEVATVMVFAAEEFPADRRGFVLGVIQGTSSLGAIVCAGVTPLLTATT
                     LGWRMVYLVGGVPLLLVAYLRRNLRDTARFAATETQTALPLARVFQSRYLPRLLLLSL
                     IWALVMFCTQSALLFWKDFAVTERHFSAAQVAQCMTIAAVGSLPLVFSVGKLLDVVGR
                     KASAVVVFGLTILGVLLSYTRSSQMLLTLGVLLGIAGTTCMLSVVQTLTTELFPTEIR
                     AESFGVANNLIGRTAGVLSPLLIGFLAVRFGYGPAVQSTAIGPLFAMLLILLAVAETR
                     GKELEETAALPD"
     gene            complement(36092..36601)
                     /locus_tag="PKL17_04345"
     CDS             complement(36092..36601)
                     /locus_tag="PKL17_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNK43989.1"
                     /translation="MSSEKQSPAPAHSHSKPTGEHASHNHKEQARSLLGKGGLRITAP
                     RLSVLTALIDSHRPMSAQEVIDKLAPAGIDHVTVYRTLNTLVDAGIAQAVGTTERGRR
                     FEVHACEGCRVGHPHLECRRCGHLVCLEQGLLPTMVVPTELAGFQVEEARLYLYGTCG
                     SCQAVAKAK"
     gene            complement(36604..36786)
                     /locus_tag="PKL17_04350"
     CDS             complement(36604..36786)
                     /locus_tag="PKL17_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNK43990.1"
                     /translation="MANTQKLKRYRCLVCEHIYDPAVGDPDSGVAPGTPFESLPDTWI
                     CPDCGASKFDFEPIAD"
BASE COUNT         7238 a        11438 c        11207 g         7195 t
ORIGIN      
        1 ttccccgaaa tactcgatcg tccatggcgg ttcctgaccc agccgattcg atcctccgat
       61 gaagactccg tggcgaaccg catcgacgaa aaactcttgg tgccagacct tctgactctt
      121 tttcccaaac gtaatcgccg gacagaaaac aagctctgcg ccgccttctt tgagggaatg
      181 gactacgcct tcaaagtgac ggtcgtaaca gatcgcgacc ccgatgtttc ccacgctggt
      241 gcggaagacc ggaaagtatg gattgcgggc gatgttggcg ggatagtgac ccaagtttcc
      301 gtcgctcggt ccgtaataaa acgtctcgtg aaatgatcca cgatcgttct gtccgcatgg
      361 gatgtgcgtc ttgcgatact tgccgatcac agacccctgt tcatcgatca caaccgcagt
      421 gttaaaccgc ttgccacttt gggaatcggt ttcaaacagc ggtgcaatga cgatgatgga
      481 atgcttttgt gcggcttcac agaatgcttt caccgacgga ccagtatgtg catcttcggc
      541 aaaggccagc cacatcggat cggtgtacag cgcaaagtac ggagcggtgc atagctcacc
      601 caaacagatc acccgagcac catgcgcaga cgccctcgcg ataagtgaaa tgttgtgatc
      661 tacgtttgcc gcacgaaccg catccaggtg tccacgcagc gaaggaagcg cttccaccgt
      721 aggaggcatc gcggcgtagg cattctttgt ctgtgtcagc gcgcaacgga cgatgcggcg
      781 ggcagtcatc gggcgactcc agtcagcaga atgaggacgt tttcatccgg gtgaagcgga
      841 ctgtgtttgt gttctgtaag cagtcccgca agacctgcac ttcccgtcgc agaaaccgcg
      901 attccggtgg tccgtttggc cagatcatgc gcagccaaaa tggccgattc cggaaccgtc
      961 agcgcgtctc cgtttgactt ccagatttct ttgacacagg gcagccaatc gtaggtttca
     1021 tcatccagga tgccgtgcgc agtactgtgc ggaaccgact cccatggcca catgtatttc
     1081 gctttgtgcg cagcaatctg tcgccactct gcatcactta ggatccgtcc ctgtggcccg
     1141 tattctgcgc gcagcttggc cagcgcacgc gaaagcggcg acaccgattc tgtctgcact
     1201 gtaaaaaagc gtggacgatg cgaaaggagt ccagtacaca gtgccgtctc gacggccagc
     1261 gcgcagcttg cagccaaggc tccaccgccg acttgcacaa aaattcgcgc cggaatggtc
     1321 tcaagcgaaa ccatgtcact cagcgcttcg gccatcagtg tctgtccacc atcgagggtc
     1381 agaccgttgt ccggaccttg gcaacagaag ggaatcgcac ctgacttgac ggcttcgaca
     1441 aaacgtcgat aacagggatc gcccgtttcc ccagccgacc gcgaacagac agtgatcgtc
     1501 gcgcccagtt cacgcagaga agacaccaca ctggcttccg catcatccgg aatgaacacc
     1561 tgaagcggcc aatgggcggc ctttgcaacc acagccgcag ccagtgcagc gtttccacaa
     1621 ctggcaatcg caagcggtgg acgatgtgtc ccagagcccg caagggaaag ggcttctccg
     1681 acgagaagtt cgaccatgat gccgaacagg tgccgacctt tgtgtgaccc gccggggttc
     1741 tgtgtttcgt cttttacata cacgccaccg cgctcgctca ggccaaacca atcagacagc
     1801 gcgtcactgc gataaagcgg ggtatgggaa aatgacttgc ctgtaaggtg ggtcacctgt
     1861 tcggtcagtt tttcgacggt ggaatgatat agctcagcat gtgccagtcc gtattcgcgg
     1921 cagcgatgat aggccagcat ccgatccgca aagcggagga agggatgcgc aaacgccgga
     1981 gttcctccca ccgaaggaag cggcagatag ggtcgcagaa cgtgaaaccc accgtcgctg
     2041 tgcttgggac agacgaacgg aggcattcct tctgtcaggg gaacttcaaa cccacatcca
     2101 gcacagcgca aagagatcat gctacgccat cctgcgtccg agcgttcatc gccgcaatcg
     2161 cttcatccca tcgtggcaca ttctgccgca gcgcgcgcca gaatgactga tgccgacgag
     2221 cttcaaagtc tttgagcgca attccttgct gctcaaccca ggtaaaatac cccagattga
     2281 agatccgctc gcgatcacgc agcgtcagtt cctgcatcag atcggtgcga accccgagca
     2341 ggtgctgacc gaacgtctct gccgcggcaa gctgatcaaa tcctgttggg aagtagcgcg
     2401 cttcgttttt cggaatctct gaaccgtaca tcgctgcact atcagtcgcg acggtgacga
     2461 cggcctctgt ttccggtagt cgcagatagc gcgctgtttt gatcgccgcc aagatgttgc
     2521 agattcccga aaatcccaga ccggaaagcg atgcaacaag ttccggggat agcttgcgcc
     2581 gatcgcgcag atagcgctgt ccttccggat gattaaacaa caggtgcaga ccgtcggtgc
     2641 tgtgatcgga aatcgccacc accacatcgg tgttcatcac gttgtgaatc agcggaacgt
     2701 gcttgtctcc gattccctgg atgttgtgtt caccaaatcc gttcgcaagc agtgtgggac
     2761 attcggctgc ttcgactgca acgatccgcg caccgtgttt ttctttcaga taatcgcccg
     2821 cgccgatcgt tcctgctgat ccggtcgcgg acacgaatgc ggcgacttgc agaccactgt
     2881 ttgcaagtac cgcagcctcg gcagcacgtc cggtacagtg ataatgcacc agatagtttc
     2941 cgtactctgc aaactgattc aggatcacgt tctgtggatc acgacgcagc tcgttgcact
     3001 tgtcgtagat ttccttgacg ttgctttctg ttcccggcgt cctcacgatg tcgctgtcat
     3061 cggcgaccca gcgtgacagc cagttgaagc gctctgcact cattccggct ggcagaatcg
     3121 cgacaccgcg gcagccgaga attcgcgaaa tcgcaacgcc accacggcag tagtttcccg
     3181 tcgacggcca cagtgctcgc tgcgtagttg gatcaaattc ccccgcgacc agacgcggaa
     3241 ccaaacagcc gtaggccgcg agcactttgt gggccgcgat catcggaaaa cgagatccca
     3301 gcagcaccac aatttttgca tccacgccag tcagttcttt gtgaagcacc aggtgggccg
     3361 gaaccgactg gatgccgcgc cgacttgcat cgttatacca gtgtacgcgc cacagattga
     3421 gtggatgggg ggcgtccgga tcgacatcgc gcagggccgt ctggatctcc gcaggaatca
     3481 gaccaggatc agcaagctgg gaaagctgcg gcagcagtac tttgcttttt cgcagacgct
     3541 gcatggcgtt gtgaagcgct tgggaatcgg ttgggagtgt tgcggaagac atcgcaaaaa
     3601 tccttgggaa cggatgtgtt tgtcgtcgaa aaataagtta cagcgcagtc attcggttcc
     3661 acaggtggaa cgctttctgc tgcgcatcgt gttcgatttc ttgtcggtct gcggtcacaa
     3721 gtgtcccatc gcggacaacg agctttccgg cgcagaacac atgccggact tggcccgatg
     3781 gatcgcgcac aaccagatcg gcaggcgatc ccacagcaat ggcaaaagcg ggcagggcga
     3841 agcgttccgc aaccaggatg tgaccagact gtgcgcgccg tgaaatcacg gagtcggctt
     3901 caccaaattc ctttgcacgg gtccgcagcg ccgcttcttc ttcggccatg ttcgcaggat
     3961 atccgtctgt cccaagcgcg acgcgagcgg aacaggacag cgcgtgcgga taccccacgc
     4021 ggttgccgaa gttcgatcgt gggttttgta ccaaccacag accttcgtcc tgacagcgcc
     4081 gcaccgaatc tgcatccaaa tggacaccgt gcgccacaat cgaacctggt ggcagcgccg
     4141 ccagacgcag caaacgatcg agcggatcgc ggtatccgcg ctttttggca tcttcgacat
     4201 cggccaggtc ttcggcgaca tggacgtgca tcaccgttcc cagctcgcga cagagctgac
     4261 ccgcagccaa gatcgtttca tccgaaacgg taaagcttgc gtgcagtccc acaagtccac
     4321 gcacaaaagg acgctgattg gtcacgatga aacgtcgaca ttcggaaaga ccgtcgtctc
     4381 cctcgcgtcg accaaagttc cgttccgtcg ccccataggt gagcagagcc cgacagccca
     4441 gctctgcaca ggcatccgcc aaaatatcga gcgatccggc aatcagcgca ggcgattcat
     4501 ggtgatccac aagcgtggtc gtccccataa gaagcgctga agagatgtaa tatcgcgccg
     4561 ctgctcgcaa cgaatcggca tcgagtgcgc gatccaagcg ccaccagatt cgttccagaa
     4621 tttgcacgaa attttgtgga gcgaccgtcg gtgctggcat tccgagcgga gccagaccgc
     4681 tgtacagatg ggtgtgggcg tttacttgtc cggggaataa agcgcagcct tcgcagtcga
     4741 tgatttgtgc gtccccgatg acgctgtggc tggacacctc ggtgatgcga tcgtcacaaa
     4801 tgcgaacatc accatactgg gattgacgga gcagccactc ggacataggg catcctattt
     4861 tttcgcgtca cccgtcgctg gatcgttcgc caccgggact tccgccacag aggatgaagc
     4921 gggaattacg atcggccccg gtgccgcagg catcaaaacc ggcgagacgg gcaccgcgag
     4981 cgaaaccgga gaggccgcga cctgtgcgat gggagccgcg actggcgctg aaggatttcc
     5041 ggttctgtcc ggaatccgtc ctcgatcttt tgcgcgtccc gcatcctgcg gtttttcttt
     5101 tttcagatcg gtgctagcgc cgcggatgtg gaactgctcg atgtgtcctt cgaccagatc
     5161 aaagcgctcg cgcggtttga tgatgatctt cttttgcagc cgcttttcga gcgcttcgat
     5221 gtactcgttg tccgcggaag ccaggagttc agcgatttcc ggatgggtgt gcaccacgat
     5281 tgtgtcgtcg gcaaacgcat gtccgtcgcg ccgaatgtgc cgcagcactt catacgcgat
     5341 cgtcgtcttg gatttggtgt agcccttgcc atcgcagtac acgcactgct cggtcagcat
     5401 ccggcccagc gactcgcgcg tccgcttgcg cgtcatctcg acgagtccca gctcggaaat
     5461 cttggtcacg ttggtgcgag cgcggtcggg tttcaccgaa tcgaggaacg cgcgcatgac
     5521 cttttcctgg ttggcctcgc gatccatgtc gataaagtcg atgatgatga tgccgccgat
     5581 gttgcgcagc cggagctgat ccgcgacttc tttgcacgcc tcgaggttgg tcttggtgat
     5641 ggtctcttcg aggttgcgct tgccgacgaa cttgccggtg ttcacatcga tcgcagtcag
     5701 tgcctcaccc tgatcaatga tcagcgtgcc gcccgacttc agctgcactt tgcgctcgag
     5761 cgcgcggtcc agctccatct cgatcccata cgcatcaaag atcggctcgc tttgctcgta
     5821 gagatcgatc ttgctgacgt actccggcat aaacgtgctc gtgaacttga tcagccggtc
     5881 atactcgggc ttgctgtcga tgatcagctt ttcgacgtcg ctggtgaaca ggtcacgcac
     5941 cgtgcgcaaa agcagatcaa ggtcgtgata aagcagcgcc ggtgccttgg ccgcttccga
     6001 cttcttcacg atcgagttcc acagcttgat gaggaactcc atgtcggcgc gcagctcttg
     6061 ttcctgcacg ttttcagcga cggtgcggac gataaagccc gtgcccggcg gacgcattga
     6121 atcgacgatc gagcgaagtc gcttgcgctc tttttccgac ccaatgcgac gcgaaatgcc
     6181 aacgtgatcc accgtcggca taaacaccaa gttgcggccc ggcagtgaga tgtacgaagt
     6241 cgaccgcgaa cccttggttc ctatcggtgc tttggcgacc tggacaatga cttcttggcc
     6301 agggcgcagc aagtcttcga tgcgggcatc ccggcttttg tcgtcttctt tgcggctgcc
     6361 ttcgtcgtct tcatcgacaa agagcgactt gatgtcctcg ggggcgccgc ggacatcggc
     6421 gacatacaag aatgcagctt tgtcggttcc gatgtcgacg aacgcggcct gcatgccggg
     6481 cagaacccgc agcaccttgc ctttgtagat gttgccgacg atccctcgct cacgtcggcg
     6541 ctcgatgtag agttcgacga cttggccgtt ttcgaccaag gccacccgcg tctcgggtcc
     6601 gtcggcgttg atgacaagca agttgggcac gataccgtat gcctatgaaa aaagtttcat
     6661 gctctggcga ccgctctgac accgatgtca ggctgcgggt gggtggcttg caaagaccac
     6721 tcgctgcgag caaccccgcg taccacgagc cagcctctct tttcaaggaa atcacttggt
     6781 tatcggctcc acctgcgcgg gctgacatgc gctggcacag gccttgctca agtatcaggt
     6841 caagtagtta cttcgcttaa ccttggtgcc gcgctctcgt ccttaccttt atcaaacgag
     6901 agcaaagcaa cttggagacc acgcccaagc gctattttta actccgcttg gggtggatca
     6961 cccggcaacc tcacatttcg gtgtgacgag tcggtgagac ggcctgcctg aacaaggcgg
     7021 gccgtttctg ttttggcgct acggaatctg catgccgcca gacacattga tcgtctggcc
     7081 ggtcacgtaa tccgaaagcg gcgacgaaaa gaacagcacg gcgttggcga tgtcttgcgg
     7141 ctgaccaaag cggcccatcg ggattttttg cagcgtcgct tgccgctgtt cttcgggcac
     7201 gccgagcaca tcgccgggtt ttttggccgc ggtcatgcgt gtttcggtaa agcctggggc
     7261 gacggcgttg acgttgacgt ggaagcgggc ccactcttgg gcgacgacgc gagtgatgcc
     7321 gacattgccc atcttggccg cgacgtagtt tatctgaccc ggattgccac ggattgccgc
     7381 cgtcgaaaag aagttgacga tcttgcggtt atagcgaggc tgcccaagct cctccagttc
     7441 gcgcttggcg acttcgcgaa tgtgaggcgc cgccgatcgg atgcagttga acgtgccggt
     7501 caggttgacg tcgatgacga gatcccacca ctcgtcgggc atcaaatggc acatcttgtc
     7561 gcgtgaaagc ccggcgttgt tgaccaagat gtcgagcttg ccaaagcgat cgaccgccgc
     7621 cttcatcagc tcgtcggtgt atttggggtc cgtcaccgaa ccaatcgacg ccaccgcact
     7681 gccgggccgg atcgcatgac actccgccac tgcttccgct gccggttcgg catccagatc
     7741 gttgatgatg acgctggcac ccgccgacac aagcgtggtg gcgcagacgc gaccgatgcc
     7801 gcgaccggcg cccgtgacca ctgccactcg atcgtgaagt aaacctcgca gaaccgtttg
     7861 atcgctcatc gcttgggatt gcctttcgtt tggtggaaag agggttggga cgtgcgaaga
     7921 cgtggccgtc tactatcacg gcagcgaggg gatcgacaat tcggcgtggc acgactcttt
     7981 gagcgcagcg acgattgcat cgagcgcggc atgcgctttc gacataaagt ccggcgcgtt
     8041 ttcgataaac actcggtttg gatgatcagc ccaagcttgg gcaatccgct cgtcgatggc
     8101 gtgcgcctct tcagcacttt cgatgcgcag cggattttgg tggttgtagc caacctgggg
     8161 cgttcgcagg tgaatcaccg tcgaatagcg ccgcagttcg tcggccttgt ttgtcccgag
     8221 ctgtgaaaaa aaatcctggg caggaccagg ccaataggcc agtccgtcga gcgtgccgcg
     8281 gtcacaaatc gcgcagtgga ccttgccttc ctctaaagcc agcgcttcga gctcatgctg
     8341 aacatgaaag attgcgcgct gtgccgcccg acgcgccgcc ggactgtccc gccgtggaaa
     8401 tccgccagcg aacagcaggc tcgcagcttc gggcaaaacc gcaatgtgat ggtttagcat
     8461 ccggcttaca atgtcgagca ccgcggtctt tccagccccc ggaccgcccg tcagaacaat
     8521 cagtcgcatc tggcaatcct gactctgctt cgcacccaag ccgtcaaccg ttccctcgtc
     8581 cataacgtgt cagcccacca gagcttgcgc gccttgtcaa tcggctcaat cggggcgttg
     8641 acggacaaaa ccagccccgc gtagcatcgt ggcccggagg aaaagccctt gcttcacaaa
     8701 ccgcaccgtc cacgtttcgt caggctcagc gccgagcatg cccgcgcagt cctcggtggt
     8761 ggcgccgatc ttgagccgtg gatggtcatc agcaacggtc gcttcgtcgc caagcagcgc
     8821 atcacgatga tcggcccgcg cggacgcatt gatggcgtcg ccgtcgtggg accactcgtc
     8881 gaatccaccc aagtttcact cgccgcgcat gatgaggaaa agctcggcct cgccgatgat
     8941 cccggtcgtg gcgtgctttt ggtcggacct ggcggcgaag cgaaagtcgt ggcatccgaa
     9001 tagccatgtc catacgcata aaccgtgtct atacaaggac cggcgacgac ggcggcacgc
     9061 acctcgtggg tggtcaccgc gtttccaaag acgacctgcg catcgagtgt tacggcacag
     9121 tcgacgaact aaacgccaca atcggtctgg ctcgcttgca cgccaacacc ccagcgacgg
     9181 cggaccttgc ggccattttg gaacgggtgc aaagcgaact ctttaacttg ggctcgcagc
     9241 tcgcgacgcg ggccgaagac ttgcgcccga cccagccggt gatcctggct cgccacatcg
     9301 atcgcttgga acaggaaatc gatcgctgca atgagcagct tcccgcccta accagctttg
     9361 tgctgccggc aggtggtgca ctaaccgcgc atctgcatct gtgccgcacg gtttgtcggc
     9421 gtgcggagcg acttgcggtt tcgctcatgc atcgcgagcc gctttcgccc gaagtcgtcc
     9481 agtacttaaa ccgtctgagc gacgcactgt ttgtgttcgc gcgtcacgcg gcgcgtgtca
     9541 ccggcgaagc ggaagttctc tggcaacccg aaaaaaccta gtccgctcaa gtccgccaac
     9601 tttcccgttc tggggtctgc ctttccgtcg ctgcatgcct acattaaaat gggtaggtgt
     9661 gttctcaatc ccgagagcaa aaaagcgtgc gaattcgcga aaacttcctt gacgtgtggc
     9721 agggcggttc gcagaatgcc ggtgcaagtc aaggaaattc gtagggtttt ccggtcgaat
     9781 ttgtgggtca gttcgctgtt gaccccccgg aatgttgacc cacctaagga gaacatcatg
     9841 ggtactctgc ttcgcacgct ggctctcgga atcgctttcg ctgcaaccgt ttcgggctgt
     9901 ggccagggca atgacgacac tgacatcgac acgctgcccg tcgtcaccga ggatgacaag
     9961 gccgacacga cggggagccc actcatttgg gtccgcccca gctcgttccc gatctactgc
    10021 atcggctacc catgtgcgac caagcaagtc gccgaagtga acggcggcga aggccggctg
    10081 atcttcaagt acgactggcg cgcgctcaag ctcactgcga cggagcagac gacggcggaa
    10141 gccaatcact ttaatatgct gctccgtggc cgttacaaca ccgtcaaggt gtcgggccag
    10201 cccatgacgg tgctccaaat ttcgcgcgca agcgtcaagg tcagcagcag ctccgacgac
    10261 aacgccgaaa aggacagctt ctatctgacc aaggaagccc cctgctccaa gcagccgtgc
    10321 cccgcggtcc aggcagagat gatcaacaag acggcggcgc cggaagtgtg ggccacggtc
    10381 gatctgtcgc ggcttggtct tagcaccacg cagcagcagt cgctgctcac cgaaatgcaa
    10441 gacaagacgg gtcaggtcta cgtgagccaa gcgaccacgc cgacgggcag caagcagatc
    10501 agccaggcgt tccgcagcat caagtccccc gcgctcaagt agtcatcccc gagcggccct
    10561 ttttcgcgcc gagcaaatct ggccatcgac cttcacaatt cggtggccaa gcgagctctc
    10621 ctgtgtagga tagattctcg acggcagtgc tgtcggatcg agcgagcagg aggtcttcta
    10681 gtgtctacac tgcgcgagaa agacgaaaaa gttgctcaga aaaaaggcct gatggcggga
    10741 gccgctgtcg tggcatcagg agcagccgtg gtcgtcggag ccccggtgct tggcgtactg
    10801 ggtcttcttc cggcggctta tcttacttat gactggttca tgttccgcgc caagcgaggc
    10861 atgcgctttt agtcgctgcc ggagcgaagg tttgtgagcc tctgtcccac ctgcaagtcc
    10921 aatttttctg gcggcgagca gttctgtcct cgtgacggta caccgctgcg gccgtcgctg
    10981 cccgacgatc ctctgtcagg tcgggtgctt tcgggccgct atcggctgct cgagctgatc
    11041 ggtcggggcg gaatgggcgc cgtctatcgg gcgcaccaca tcctgatgga caaacccgtc
    11101 gcagtgaaag tgctgcgcgg cgagctgtcg agtgacaccg aggccgtcgc gcgttttcac
    11161 cgcgaggcgc gctcggcgtc acggctcgat cacgagtgca tcatccgcgt caccgacttt
    11221 ggccagacag acgacggctt gctgtttctg gtgatggagc tactcgacgg agaaaatctg
    11281 gcgcaggtca ttcggcgcgc agctctgcca tggcgacggg cagcgacgat tgcccgcgac
    11341 atcgcgcaag ggctggccca cgcgcacgag caaggtgtga ttcaccgcga tctgaaacca
    11401 gaaaacgtgg tgattttgcg acgaggcaag gggcgacagc tcgtcaaagt gctcgacttc
    11461 gggctggcca agctgatgca cgaagcgggg ccgtcgggaa gcgctcccga tgaggagcca
    11521 ggccgggcgc tgaccaagac gggcgtcgta ttcggaacgc ccgagtacat gtcaccggaa
    11581 caggcagaag gacacaagct cggcccgcac accgatctgt atgcgctcgg cgtcgtcatg
    11641 taccaaatgg tgacgggaaa gttgccattt acggcgccga cttttttggc cctgattgcc
    11701 aagaccgtcc atgaaccgcc cccaccgccc agcaacgtgg cgcccgatgc caagctgccg
    11761 ccagggctcg aacagctcat cttgcgctgt ttggaaaaat cgcccgacga cagaccgaac
    11821 agcgcagagg aaattgccga cgaactggat gcgcttctgg cccagaactt ggatgacgtg
    11881 ggagacgatc gggcgactcc ggcatccggg gcagccaaac cgacggcttc agcgtcccag
    11941 ccagtcgttt tggcgccagc cgccgttccg cccggtgggg ttgcaaagac cgtgcagcgc
    12001 gtaaatccag cgcgtccggg cggggtgact ccatcgacgg ctgcggttgc aagtgcgttt
    12061 gaagcgctgg ccaagcgcgg tcagcctgtg agtggtccgc ccaaagcgtt gccggtgcca
    12121 gccggacccg aagacgacgc gacagctgcc gcagccagcg atgccggtgt ggtcgacgac
    12181 agcgcgccgc cagccgtcac agcgcctgcg gtgtccagcc agagtccatc gccagaagct
    12241 acgacggtgg ccgatccaga acctgcgcgg gctgcgccac ggaaagaaat ccaagaagcg
    12301 ccatcgacca gcagtggacc cgttgcggtg ccacgtagac cgtactggct tgtcggcgcg
    12361 ctgcttttgt ccgggatggt cggtgcaggg gtttacttcg gtcccaaact gctcggcaaa
    12421 aattcgcagc cggtcagcgt cgacagcgac gaagttcgcc aagcgaagct gctgcttgca
    12481 accgacaaga gtcccgctgg cattgaagcc gcgattcgtt tgctgctgct cgcgcgccaa
    12541 aaacaggaaa cgccggagct ccatcgcttg ctgtcaggcg cctacgaagc acagggcaac
    12601 cggctgcgtg cgcttggaca catgtacctc gccgtgcagc gttcccagac gatgtccgag
    12661 cggtcggtat cgcagctggc gctggcacag cttttgtctc ggcttggaca cagccaagag
    12721 gcctgtcaga cgacgcagcg gctgttgcgg gaaaaacctg actcatccgg cgaagtgaag
    12781 gtccatgcgc aggcgctgct ttccgcgctg cgctgcgatg ccaagtagcg ataccgtgtg
    12841 cgccgggttc cgcttgcgga tcaccgccgc gatcggcgtt cgtcactact tttcgggtat
    12901 cgccaggctc aggatcatgc caccgatgag caggcccgac accgcatagc caggtgccca
    12961 caagccgacg gtcgtcagca cacagttttg gccagcgttt gcttgcgacg aacacggtag
    13021 tccgccggga ttttgcgcca gcgtcgagtc cagcctctta tcaaagtaat gtgccgcgat
    13081 cgtcccaccg agcgccgcaa agccgagcgt gccaaacacc gcagccagcg ccttccgttt
    13141 ggtcgataga cccttgcgct gctgcggtgg cgtcccctgc ggagtctggg tccacagcgg
    13201 tgcttgcgtc gggtacagcg gcggtgcact cggggcagtt tgggctgtcg acgggagctg
    13261 cggcacatac gaaccagcca gcactggcgt attgccctgc ggcggcagtg ggggcggaac
    13321 gtttggactg ggcgcctgcg ggctcgcgac cggcgttgtt gcaagctgca ctttcgcgct
    13381 ttcggctggc ttggcgaccg gcatcggcgc aagctgtgga cggctgcttg ccgtcgaatc
    13441 ggtgccaact ggcgcggcgg tcttggccaa ctgcggcggc ggcgtcaccg ggacaagcgg
    13501 cggcaccatc acaggatcac tggctggcgt cggaatcgct ggtggtccgt ttttcgccgt
    13561 gccttccagc agctcattga gctgggcgtc ggcatcctga cctttgtgat agcggctgag
    13621 tatctcgctc accgtgcttt gcaccagcag cccgagcttg gtctcgtcac agtcgttgca
    13681 caggttttcg acgtcgaaca cctgacggtg acggccatcg tatagatgca tgaccacccg
    13741 ctggctcttt ttgctaccca gttggaagat gtcgccgctt agcaccacgt aggcttgatg
    13801 ttgccgggcc aaagccgaca gacacgcggc agtccgacag cgccgatccg catcggtcag
    13861 cgtcgattcc accacccgca gctctttgcg accggccagc tgttcgcgca cttgctcgct
    13921 gccgacgcca tcgagcgccc ccccgcgctg aatggcgagc cacaaaagcg gctcctctcc
    13981 atcggctcca gccgtcttcg gcagaaggac aaacagcagc agcgcacaga gccagcccca
    14041 cgacgagccc gcagtcagcc gcactggcac gcgatgggaa ggttgcttct cagttacaag
    14101 gggcatgagc ggtcacgtct cgcagatctc tctattcagc ataaactgaa attgccgcgt
    14161 gcggggaggg cggatttcgt caaattttcg cggacttagc caaccaggag ccgaaacagc
    14221 ctctcgaaac ccgtggtctt gcggttcacg gcgtatcgtc ctgcgcggcg actgtcctgc
    14281 gccgctggtc gcgagcaagt cgtgatagca tcacaagtta tggacgcttc cgaaagctgt
    14341 cgtggcgatt ctctcgggct tgccgcatgg gttcgagccg catttttgca agtcgggttc
    14401 tggcttttgg ccgctgggat tggttttggc gcagcgactg tcacaagctg cgtggttccc
    14461 gacccggcgt attgtggcaa agacgaggag tgcgcaggcc gtcccgaatc atccgacggg
    14521 accatgttgg tttgccatcc gaccaagcac atgtgcatcc cgaacaaacc gggccagtgc
    14581 ttcaaggaca ccgactgccc aagcaccaaa gcgagtcact gtgacacaag cacaaaccag
    14641 tgtgtttcgt gtcgcgttgg agatccgact gatacctcgt gtgcatcact cgggctcggc
    14701 tcgatgacca agtgtctcga caccccgtcg ggaccacagt gcatcgaatg ccaacagcac
    14761 ctggactgcc cgtccgaacg accgatttgt gacgggggat cgtgccgcag ctgccgcgag
    14821 cacaaagact gtgaaggcac cctaaactgc gaaggcggcg tcacctgcac tgactcgatg
    14881 gtctgcatcc gagacgggga cttgcccgaa ggtcgcgccg gtagctgcgc ctggaacggc
    14941 cccggatcga cgggtcgagt cgtctacgcc cacaacggca acgcggcgtg cagcgacacc
    15001 gatccttcgt atggattccg cttcagcgag ccggtctgta atctcgcccg cgcgtttgcg
    15061 attgcccgcg acacgggtcg tcgctacatc cgcatcctcg gcagcaacta cgatccactg
    15121 aacgccacga ttttgtttgg cacctattcg ttcatcggcg caccgggcaa aaactatccg
    15181 accctggcga cgatgaaagg ccgggcgctg ctgttcgaca ttcgcgacac ggcgcgcgtc
    15241 accatcgatc agctcgacat gaccgaacag aacgtcgacg cgggcgcaat tgcatgctcg
    15301 ggtccgggcg ccgatcgaat tcccggcctg accattcagc gcagcatctt gcgtggatcg
    15361 accccgccgt cgtttcccaa tcccacgtcg tcggcaatca atgtgaacga ctgcgccgtg
    15421 cggatcgaac agagcatcat cggtgtcacc aagctggccg acgcaatgat ggcttcgacg
    15481 gcaaccgcgc acgccaccgc gatccgcatt tcggacatct ggcagagcac caaggccagc
    15541 tacgtgctgg aaaacaacct gatcgcgggc aataccgcca tcgccgtgga tctgctcaac
    15601 accgatcgct tgcagcaaaa gctggtgatg cggtttaaca ccgtcgctta caacgggcga
    15661 tcgaacaacg gcaaggccgg cggcattcgc tgcgcgacgg ggccgtcggt caagccggcg
    15721 atcaccgaca acatcttttt cggcaacagc actgtcggag catcgcagat tcagtttggc
    15781 gaaggctgtt cgttcggcga caccgtggtc ggcagcgcgg attcgtgtgc ggaagcaggt
    15841 ctgcgcaaga acaatcccga gttcttcgac gacttttcgc tcaaagacgg tcccgccaac
    15901 cgcaactgct gcatcgatca ggtcagtccc ccgagcggtg acaccttccc gacgcgcgat
    15961 ctgctgcttt cgagcagacc gagcggcgca aaatacgaca tcggctgcta cgagctgcca
    16021 caataacggg ccggtttccc ctctccgatt gaaagccttg ctactcaacg cactaaaaaa
    16081 gacggcagga cgaccctttg acctctgtgg gcttggggaa tgctcggtcg atttggtcta
    16141 tcgggtgccg ctgcggcttg ataaacccct gcccgacaag gtctccgcaa aagcgcatga
    16201 agtgcttggt ggtggtcaaa tcgcgacggc ttgcgcagca gccgcgcggc ttgggcgcaa
    16261 ggttcgtttc gtcggtgcag tcggcatgga tggggcaggg caggcgcttt tggccgaact
    16321 ggccagcgat cgagtcgaca cgtcgcttgt gcggcgctgc ctgggggttc agacccggac
    16381 ggcgctgatt ctctgtggcg atgatggcga acgatctgtc atcgagtggc gcgatcaggg
    16441 gctgacgctg ccgccaggtc tgcccagcgc ggaacagcta tcgactgcac gggtgctgca
    16501 tgtcgatctg tgctttcccg acgcgacgtt ggcggcagtt gtggcagcca aggcacacgg
    16561 actgatcgtt tccgtcgatc ttgatcggcc tctgccgcgg gttgacgatc tgctgatgct
    16621 gaccgatttg tgtgtcgtat cgacgcactt cccctcacgg ctgaccggga tcgatgatcc
    16681 cgaaactgcc gccctggcgc tttccgaccg aacaaaaggc cgcgtggtgg tgacacaagg
    16741 cgagcacggc tgctggctgg tctgcgacgg tgcgattgtc cgcttgccgg cgtttgcgcc
    16801 acctcgtttg gtcgatacca cggcctgtgg cgatacgttt catgcggcgc ttatctgtgg
    16861 cttgctcgat gctgcggacg gcaaaacggg atcgcgcggt gatgatgagt cggcgctacg
    16921 ggacgcgatt cgttttgcca gtgcagcggc ggcgctcaag tgcggtgatc tgggtcgacg
    16981 tggctgtccg cagcgtgccc aagtcgatca gttcctacag accgcccact aagcgcgctc
    17041 cgactccgaa aaggtttggg cacgggggtc caatgtttgg ataatcgctc attggtgacg
    17101 aggaggagtt ccgatgaaga gcgcatcttg gtctagtctc cgtcgacgat cgtttctggc
    17161 ggccatgctg ggaggcgcgg cagcggctgc acttcccggc tgcggaagca gtccgacccc
    17221 tgcccctggc gtctcgattc ccagcgtgta cagcgaagaa tcggtcttcg attacctgca
    17281 gcgcaccgtc ggcagtttcg aacagacgcg gtatcgccag atcatcgggg cggccagcga
    17341 ctacaaacaa ggggacgaag cgattggcgt cgctgctgcc gatgaaacat cgcggcaaaa
    17401 tgcacgcacg ctcctgtcca acaccaagct cggcgacttg gctgcccatc cgctggtcga
    17461 tgaccgccag gggcagctca tcgcgcagac caccgatgcg gcgatgcaga aggcgatgtc
    17521 gtcgtggacg atggggttcc tcaagtccta tctgctggcc gcgaccgagg attcaattcg
    17581 ccaggtgaaa aacggcctga ccagcgatgt gatcggctgt ctggtcaagc ttcttaccaa
    17641 ctcgcagctc gtggcgctgt cgcagaaaat ctggaatccg ctccccggca gcaaggtcgg
    17701 cagcaaaggc tatctggggg cacgcatcca accgaactcc ccgacggaca atctcgacga
    17761 catcaagtgg cagatatttg atggttttgc ctatgccgtc ggcgatgtcg tgctcggctg
    17821 caaccccgtg tcgtctgacc ccgaatcggt cgcaagcatc gaacggatga tgcaagacct
    17881 gcgcgagacc tttggcctga ccgacatttt gccgcactgt gtcctttccc acatcgacat
    17941 ccagtcggaa gtcgagacga aatacccagg tcagaccggc atctggtttc agagcatcgc
    18001 gggcaccgac ggagccaacg gcacatttga tgtcaccgtc gaaaagatgc tccgttacgc
    18061 cgaccagcgc accggcaaat acggcctgta cttcgaaacc ggacaaggtg ccgactttac
    18121 caacggccaa gacttcggcg tcgacatggt cgtgttggaa tcgcgaaagt atggctttgc
    18181 gcgggcgctc aagcagcgtg tcgcgttggc acaggaaaaa gccggccaga ccgtcgcacc
    18241 gtgggtccac ttcaacgatg tcgccggctt cattggcccc gaggtcttcc gctttcgcga
    18301 tcagctcgtg cgctgctgtc tggaagacat cttgatgggc aagctgcacg ggatgaccat
    18361 tgggctcgac gtctgttcga cactgcatat ggatgtttcg ctcgacgatc tggactactg
    18421 cctggaccag atcatgccgg ccaatccggc ctatttgatg ggtctgccga cgaaaaacga
    18481 cccgatgctg ggctacctaa cgacggcgta ccaagaccac gttcgcatcc gcgacaggtt
    18541 tggttacaaa gtcgacgacc gcatgtgggc gttttacaag cagctcggcg tgattgacca
    18601 agcgggccag ccgaccgaca agtttggcga cccgcgctgg gtctatctgc agtttctgcg
    18661 acgcaaaggc gacaagcgca ccgacgcggt gatttacgcc gaagccgatc agaagatgaa
    18721 agaagtgcgc gagcgcgggg tttggctggc ccagggctat ggtcaaaagc cgtggaatct
    18781 ggatcccaag ctcgatgccg aaatccacgc gctctatgaa gactcccgca aggcgatttg
    18841 gaccgaatgg acgccggcct ttgtggcgtc ggtaccgatg gtcgagcagc ttcacaccaa
    18901 gtcgagcgat cgcaaagact atgtctggca tccaccgacg ggtgagcatc ttgatgatga
    18961 ctccgcgtcg gcagtggccg cgatgcggct gcgtcatcgt gaccaataca acgtgcagat
    19021 cgtcatctcg gatggtctgt gcgcgccggc cctgtcggac cccaatcacc tggcaccgta
    19081 tctggaatcg ctgcgcagtg aactgcaaaa acaaggctac aagccgtctc ccgacaacgt
    19141 ggtggtgcga ggtgggcggg tccgcgctgg ctatcacttg ggcgagctgc tctatggtgg
    19201 gctctcaaat gccgcagcga cgaaggccat cattcacttg atcggcgaac gaccgggatc
    19261 gggccaccac gccttttcgt cgtacatcac cgcgccgccg gtcagcactt ggtctcagcc
    19321 tggcaaagtc gatcacaaca tcaccaaagt tgtctcgggc atcgccgaca ctgcctatga
    19381 cccgcgaagt gccggcattg agacggtcac cattcttcag cgaatcgcgc cgccgtagtt
    19441 cgatcgagtc gttacgctcc cattgacaga agcgcgcctg cccaactaag ttccgctcgc
    19501 atcgacaaat tgcagttctc gacggtggcc ggacctatca cgccacctgt ttggcgagca
    19561 caagtgacga aggagcgtgt ttctgatggc aaagtcggtc ttgggtagcg tgttgcgact
    19621 ggcgagcgtg acggttctgc ttagcgccct tctgtcgggc tgcacgagcg gtggcggcag
    19681 cgggccgatg aatccggcgg ccaaagcgga tatgtcggtc gcgcctgagt gcgccacgat
    19741 gaaatgcgaa aacccatccg ccatctgctg cggcggcgag ccctgcattg atgtgctcag
    19801 caacacccag cactgtggcg gctgcggcaa gacctgccga tcgcgcgaag cttgcaacaa
    19861 tggcaactgc gtctgcgctg gtggtggccg gacatcgagc tgcaaagtca ccgaagcgtg
    19921 ctgtagcgat ggctgtcacg acatcctgag cgacaccaaa aactgtggtg gctgcggcgt
    19981 ttcgtgcaaa gaaggcgagg tctgcgacag tggtcagtgc aagtgcggcc catcgggcat
    20041 tgcctgcaag accggtcaaa tctgctgcgg ctcggtgtgt tcggaccgac agaacgacgc
    20101 aatgaactgt ggcatgtgcg gcaaggcctg caagcccggc aaagcctgca aaaacggtct
    20161 gtgcgaaggc gagtgtgttg gctgcaccgc gatcgagact tgctgcgatg gcacttgcgt
    20221 caacctgctc aacaaccaga tgaactgcgg catgtgcggg aacgcttgca aggtcgtcct
    20281 gggcatccca ctgccgtgcc taattggcat ctgctctttc tcgatgcccg atatgggcgc
    20341 cagggacatg tagccaggca gaggtcgcga tgagcatttc tcttcacgtc aacgtcgatc
    20401 acgttgccac gctgcgccaa gcccgcgggg tcagctatcc cgatcccgta tgggcggcgt
    20461 cgctgtgcga gctggccggc gccgatggca tcacggtcca tctgcgcgaa gatcgccgtc
    20521 acatccaaga tcgagacgtc gagctgctgc gcaagacggt gcgcggggtt ctcaatctcg
    20581 aaatggcggc aacgccggag atggtcgaca aggcaattgc aatccgtccc gacattgtga
    20641 cgctggttcc agagcggcgc gaggaacgaa ccaccgaagg cgggctcgat gtcgccggcg
    20701 gtgcgcgact cgcggtgcag gaagccgtgg cggcgcttca cgaagcgcgg attcccgtca
    20761 gcttgttcat tgcgccagac ctcacccagg tgcgggcggc ggcccaactc aaggtgccgc
    20821 gaatcgaact tcacaccggc caatattgtg aagagacgct gcgccctggc catgcagccc
    20881 aagccgccaa ggagctagag cggcttgcgc aggcggcaga agatgcggtt cgactcggca
    20941 tccacgtcgc cgcaggtcac ggactcgact atccaaacgt caagcctgtg gtccgcatcg
    21001 ctggaattga ggaactgaac atcggccacg ccatcgtcgc gcgggcggtg ctctctggga
    21061 tcgagcaggc ggtgcgcgat atgattgcgc ttatccgcca aaaccaatga ccactgctga
    21121 aaagatcgcc ggttttctct ccgctccgct gtttgctgtg gcaggcgcga gcaacgaccc
    21181 tgacaagttt ggctatcgct gcttcgtcgc cctgcgtcgc tcgggacgag ctgtgcatcc
    21241 actcaatccg cgcgcggcgg agatcgcggg cagtcccgcg tatcctgcgc tgcgagatgt
    21301 gcctggccag gtcgtcgcgc tgtcggtggt gacgccgccc caggtgacag aacagatcgt
    21361 agccgacgcc atcctggcag gcgttcgtta catctggatg cagccgggcg ccgagagccg
    21421 caccgccatc gccaaagccg aagcagcggg gcttgtggtg attgccggcg ggccgtgtgt
    21481 gctcgtcgag ctggcgaaac aagggtcggt ctagcggccc gcgtagagcg cgcttaagaa
    21541 gctctcccag cccgacaaaa gtcccgtgtc actgcgccga taaaacaggg aaggcgcaaa
    21601 gaacggacgg cgctcggtcc attcctcggg ggtgctgggc agccaggcgg aaatgccagc
    21661 cagaccgcga aagcgttcct cttcatagcg attgccggca gccagagaca gcacgctgct
    21721 ggccagggcg ctctcgctct gatcgatggc actgagcacc gcgcggcagc ttgggctttc
    21781 gctacgtgac gcttctttgc gcacgaccgt ccgcaggttc agcaaaaaca cccccaggtc
    21841 gcgtgtgccg ccggggaagt cgggcagggt ccggctggca ccaaactgca cacccgtctt
    21901 gaccgtcaga agctgctgaa tcgccaccga gcgtgtcgga tcttctgcca ggtaggccgc
    21961 cagcgcttgc cccagcgact gcaggcttgg tgacagtcca gtgcgcagct gcgacagctc
    22021 cacgacagac aaaagaaagt tcggtccccg ggttgcccca ggtgcggtgc cggtttggct
    22081 catgctgatc gccggcagct tgagcgccaa cacgcagctg cgatcgtcgc gtccgcagcg
    22141 gcttgagtgc ggcaccgatt cgggagcgat gtccggactg accagcgatt ccaagatcgc
    22201 accgtaaggc aagccgtcgt aatcaacctt ggtttcggtc gccacaaagt aacgcgcagt
    22261 gtcggccagc tcggtgctta cttcgacggt ttgcatcagg caggcatcgg tcgccagcag
    22321 gtcaaacggt cgcccactga gcttctcttc ggatgccgag cgcagcgcat cccgcaagct
    22381 cgggatgtcg aacaccgtgc gctcgctttc atcgggggcg ataccgccaa gtggccggct
    22441 cgggtcgtag atggcccgct gatccttgcc cgcgcgtggt cgccagccga gcccatgacc
    22501 ccaaacgatc accatgtagc gccgggacgg gtagtgatcg atcccccagg ccaggaagct
    22561 gcgcaggctg tcttggggcg gctggttttc atcgctggtg tattcgacga tcggcgaacg
    22621 cagcgtgctc gggtcatcgc tgtcagccgg actgtcgccc agccccagga cgtgcagacg
    22681 acgaatccct ggctgcttct gcaggtcgat ctgcacgatc agatcggcgt ccccatcggc
    22741 ggccagccgt cccgcccgct caatcgcccg caaggtctgc agcgctggct tctgcagatc
    22801 cttggtatct gccgccatat acaaaaggac cgtccactcc ttttcacact tggggcgggc
    22861 ggttcgttcg aggtagcgcc gatagccctg gttgcccagc ggtgtttgct cgctgtcttg
    22921 ggccggctga tatccgcgat ctccagccgg aagctgtgcc cacgccactt ggcagcttgg
    22981 cacgccagtg gccacaggtc ttgccggcga tgggcggggc ggatcgtcat cgcgccgcgg
    23041 cgcaggacag ccgacgaaag acaaggcaag cagggcaacc aggaaaaccc ggacggtgcg
    23101 aggcagactc aacatcggtg tttgtggctg ggtctaggtg ttcgcaaagc tgatcaagga
    23161 ctcgatgatc tttgccagag ggatgccgct ttccgccgac cgaaccgccg ctttgcgcca
    23221 gctttctggc aacggctgac ctggcgtcca tcctccggtc gcaaactgct ccgcaggtcc
    23281 aatcgcgcgc gcggtccctg ggggcgtcca cagaaaatcc ggatggcact taccagtata
    23341 ccactcgccg ccaatgatcc gcccgcttgc gtctagctcg agatcgtact tgtacacgac
    23401 cgttcggacg gcgtcatcgc caggaccatc ggtgctgcat ggggctgcca gcttttcgac
    23461 cacatagctg acttccatga tgatgccaac gaagctggcc gcttcagcgc tgcggtactt
    23521 gcggaacgga tctcgggtga acttggcccg gctgatggtg gcggtcgata gcttggtcgc
    23581 cagccgcatc gtctgcgggt tgaagtatcg gtacgaatag cccagcaccg gctggttcca
    23641 gacctcgtag tcatacgtcg cgtccatcac gaagccgcgc ttggcgaccc caatttggtt
    23701 gacgacgcac aggtgccaag tgcctgggtt tgtgtcgaag acctgtggcg acagcggacg
    23761 accattttca tccactgcgg ggtctttgtc gttgcttctt ccgccaatga accgggtcac
    23821 ggtgttggcg ttcgcccaaa gcagcgacgc aagtcccttg atatcggttg ggttaaaccg
    23881 cacctgggtt ttgccatcgg cggccagtac cgtcactgtc ttcgatgggc gcggcatcat
    23941 gtacgacgct ggagcccagc cgtggcagat gcccatccac gtttcgaccg atccgctgct
    24001 gtcaaagtag ctgcggcctt catcccacat ggccgtcgct agggttcccg tgggatcgcc
    24061 cgccagcaga tcgtattttt ccgctggcga aagcaggtca atcgcaccgg caccgccgct
    24121 ggaaagaacc gctgagaccg gatgctgttt cacataagcg tggttttctt cccatttgag
    24181 cgaaccggga aacttcggat cggcgtagcg cttgccgaga cacccctgat aaagcgccca
    24241 gtagtcgctg gaccaggggt tttgcgaaag acgcgccttg agcagcttgg ctttgtccat
    24301 ctctgcgagc cgccgataga ccaagcggtc gacgagattt tccgggcgat ccgctgcctc
    24361 aatcggggct tttcccggcg tcgcgtcttc caatgagatg accaccccgt cgatcaccgt
    24421 gtggaacgtc ttgcgcaccg agtcgcggtg cagcacgtag tgcccgttgg ccagatccga
    24481 tgcatcaaaa aacgtcgtac tccgcgtcac cgggcgtccg tttggatcgt acttcggggg
    24541 tggagtggcg gtcgcggcct tgggattggc ggcaaatgca tccaggcttt cctgtagggc
    24601 tacgtcatcc atcgcgctct cctttggcta ctgatactcg tagcttcatt acacgccttc
    24661 ccccgcgcat tgtctattca cgggggaaag ctgacgctgc gctacctgtc gatgccaggg
    24721 ccggactccg cccgattcgc tgcaagtact ggttgcggat gcttcgcgag gccgccgccg
    24781 ctgcggtctg tcctagcttg tcgagcgctt cggccagcgc atcgtggacc agcgactcga
    24841 tccgcttgtc tttgggcgtg ctcagcttct ctatcgcttg acttagaccg acggtggcgc
    24901 gctgggtctg tcccagtttg agctgcgcac gcgccagcgc ccaaagcgcc cagctttcgc
    24961 catcggcagt ttcacgattt cgcgcctgat cgagcgcaag ctgcgcagta aacatcgagg
    25021 cctgggcttc cccgcactcc acaaacgcac agcttagcgt caccagcgtc ggcaatgtcg
    25081 ccggcccgag cttgcttgtc aggccatcgc actcgcgaag acaggcgacc gcatccgcat
    25141 gttggccggc ctgccgatgc gcaatcgata gcagcgcgag caagcgcact cgatcgtcgt
    25201 tgtgatggcg caagagcaag cgatgacgca caagtgccac gaccgctttt cctggcatgc
    25261 caagctccag gtacgcttcg cccagcaccg acagcacccg tgcgctgtca aacagctcgc
    25321 tccacgacac cacttgttcc agcttggcgc tgatctgttc gacggcgcgc tggggttggc
    25381 cctgcaaaag cgccgctcgc gccatccttg caatcacccg gctttcatcg cgttcttggc
    25441 cggtgcgctc tgctgccgca agcgccgccg caaaagattc actgctctcg ctgtatcgac
    25501 gggacgataa ctgaagctca ccgagccgca gcaggactgg cacgcgggat gccgggcttt
    25561 cggcggtcgc gtgtagtcgc ttttgcgccg ccgcaatcgc ttccggtagc cgtcccgaca
    25621 gggtccacac atcggtcaag cggtcgagcg cttgaaccgt caaggtcgaa ctcttgcgct
    25681 gctcggcaag acgcagcgcg gcctcgcagg cttggactgc gcgatcgagc tgtttggctc
    25741 gcaaatggac atatgcggct tccaaatgcg cctctcccgc gaaccattcg tctctgtgct
    25801 gggtcgattg ccgcagacac accgacaagt tgtcgaccgc gcagggataa tccgcagcgt
    25861 cagaataggc ccgcgccaga tccagcagga catcacattc ttcgctgatg tcgtgacgcg
    25921 ccctggcatc ttccaacgcc agcagcagct ggggcagttt ttccgttggc tgtggcaggc
    25981 gcagcgtggg caccatgccc gacgatgaca agtcggcgtg acttgtgcct gcggctggcc
    26041 ccatcgcccc cgacaccccg gacagagagc tgcggttgcg acgtcgcgac gcccacagac
    26101 cgaccactgc caccgtcaga cacaccacga ccgccgagag cgcagtgcga cccgacatgc
    26161 atcccgccaa actgagaggc cagagcgcga gcagtgctct tcgcaacagc ttgacctctt
    26221 agcgagcagc ggctccgctc tgttccagtc gttccagcaa gcgggccgac gcctcgctca
    26281 gtcgtcgaaa caccacgccc ataccggggg gcgacgactg aacgcgcacc acctccgcaa
    26341 ccccttcgac gatctcaatt tcgtcgagca ggagggaaaa acgaacgttg acccgcgttc
    26401 ccaccggcag cgggtctttg gatcggatga atgcccccgt gcgcgagacg ttgacgacgt
    26461 actcgctcaa gtgttggtcc acggcctcga actcggcatt caccagtgtg cggtgggccc
    26521 tgggaatcat ggcctctttc gccatcgatt gctgctccct ctcttgctac agcgtgctga
    26581 ttacagaaaa cacagaagat gtcattttat cagcttttct caatcacttg ggcttgtctt
    26641 tcaccgtgac attgcatggc cccccaagcg atgctgcgcg ccatgaatcc aaaaagtgca
    26701 gatcacgacg cagacgggac tgacctgttc gcttttcaga tgccgagtcc gcttggcgac
    26761 ttatgtgggg tagcgagccc gctcggcttg tgccagctta ccttcgcacc gcttgcggaa
    26821 gcactgtccg gatacccaag tccgttgcgg cagcagctgt tcgaatcggc catcaccgtt
    26881 cccgatggcg gccaagcgat gccttcccat cttccgctgc ggccccttgg cggctggctc
    26941 aagacgcttg cgcgctatct ccacggtgag tcgctgcgct tggatatccc cgtcgacctt
    27001 cggctcgcgc gatccccgtt tcatcgcgcg gtctacgagt ccctgcttca agtggggccc
    27061 ggacaaacca ccagctatgg ggatctggcg cggatcgtgg gtcgccccaa ggcggcgcgg
    27121 gccgtgggtc aagccgttgg cagaaacccg gtcgcgctgg tcatcccttg tcaccgggtg
    27181 ctcggacggg atggatcgct gcatggcttt gcctttgggc tggaaaaaaa ggcggcgctc
    27241 ctgcgtctgg aaggggtgaa aagcaaaatg ccccaggcgt cgctcacatt ggggagctga
    27301 gtcctcgatg tgggcgcgga tttggcgctt ttctcggggg agagcttggt gataagctgc
    27361 cgagaaaccc tgcgccctct agcccagtgt ccgtgggcgc gtaggccaaa gaggaccgtg
    27421 accagatgag cgacagtagc cagcttgttc gtagctatgc cgacctgctt gacctgcttc
    27481 ggcgcgatgg cgtcccccac gaggccaatc tggaaaccag tgaagtggtg atcccaaccc
    27541 agcggggacc acttgacgcg gtgctactca ttcgctggca ggccgacgcg ggcgtcgtgc
    27601 agatgatcca gcccctgacg tttgaggtgc caccggagcg catcccggcg atggaggcgg
    27661 cgctctgtcg gctcaaccca gcgctggtgg tggctggctt tgagctggcc tacgaacgtc
    27721 ggcgggttgg cttccgcacc acgctgccgc tacagccccg cggcggactt ttgccagacg
    27781 aaatccagac ctatttccgc atcaccgtga agacggcagc ggactttctg cccacgctca
    27841 gccggatcgc cagtgggatc ggcaaaccgc agaatgtgct atccgatgcc cagtccgacc
    27901 tggcgggcgg aaacagcggt gggggtccgg gcgcactcgg gacctactag cttggcggcc
    27961 attaccaccg ggtgccttca cttttgaaaa acgtcatgtc ccagcccgat gtccatcaga
    28021 cgttcctcgc tgcgatcgaa agcgcctatc agacgctcga ttcgaacttt gacgagctgt
    28081 atagcgcctg tcacggtgcc gaagaaaagt cgcagcttcg cagcttgcac gccgcggccc
    28141 gtgatgccta ttggcgtggc gtcgcgacca agctctgcga tgacaactcc gtggttcggg
    28201 cggtctatga agacctaaac cgcgccaacc aagagctccg tgatgcactg cgtgacctcc
    28261 agcagctatc ggctgtgatc ctgcttgcaa cgcaggccgt gcgtctggct gcttccctgg
    28321 tgacgcttgc aacggttggg tagacccatg cgcgcaacgc ttcgtgggct gatactttcg
    28381 ctctgcgtcg ctgagatgac gctgcctgct ctgccagcgc tggcccagtc cgcgtcgatc
    28441 gagcaggagt ggcgcatact caccgacgcc caaagcgcct accagcgcgg caatgcagcg
    28501 gcggagcgtg gagacaaaga cggggccatc cgggagtggg atgtcgcaac cgcgaatttt
    28561 cagcaagtga tgcggatgaa cccgatgcgg accgacctgt atgcgccgct ggcggacatc
    28621 cacattcgcc gtggccaccc agcggtcgcc tatgcgctgc tcacccagca gatgcgcggc
    28681 ggtgtaaacg acctggcggt tcgcattcaa gtcgtgcgcg cgctgcgggc gatgaaccgt
    28741 cgcagcaagg ccatcgcaca agtcgaggag ctgcgcaaag accggccaaa cgatcccgat
    28801 gtcggggcgc ttcatgcgga gctgcttgcc gaagacggac agagcgatca agcgctgatg
    28861 ctgcttctgc tggttttgcc caagctgccg ctggaaggtc ggtatccagg gctcgatgga
    28921 gcgtcgatgc ggcggctcca tgcccggctg ctgcttgcca aaaatcgtgg tgctgaagcc
    28981 gttggcagtc tcacggagct gctaaaagcc aggccaaccg acgccgaagc ccagctcctg
    29041 ctcgggcaag cgctgctcgg cagcggcaaa cacgccgagg cagtggctgc tcttcgtcgt
    29101 tatcttgccc agttcccaag cgatggccgg gcgcgcgccg ctcttggcct ggcactcgcc
    29161 gatagcggtc agcttttggc cgggattgaa gaactggaac acgcaggcga agtccccgaa
    29221 gcgctgtcgc agcttggact gctctatctg cgcaaagtcc cgcccgattc gcccgcagcg
    29281 gtcgcggcgc tcaccaaagc actacagctt cagccgaaca acttgaaggt ctgcgtggca
    29341 ctcgccgagc tgcgcgatca ggcggggcag ggcgcacaag cgctcaccga tatcgagcgc
    29401 tgtaccgaac cactgctcgg cgccgatggt ggacttggcc ccgtcgatgg aactgcccaa
    29461 aaagcggtgc tgctcaggct ggatcttcag ctgaaaaaca acaagtttga acctgcgatg
    29521 cagacctggc ggggggtgca ggcgcgctat cccggcctgt cgtcgctgca aggcaagctg
    29581 gcggcagcgc ttctgcggcg cggcttgtcg aaactaccga cgggcagcca gccgtcaaat
    29641 gccgggctgg cggatctaca agaagcccac aagctggccc cgagtctggc ttctgcccag
    29701 gcgcttgcac ttggacagat cggggctggg cagccgcaag acgcgctcca gacgttacag
    29761 aactacctca aagatggctc caccgagccg cgctttctgg gcgcttacgg gcgggcactt
    29821 cgcgatgcgg gtcaggcgca ggacgcactg ctcgtgctgc aaaaagccga aggtctgaca
    29881 tccagcgcgc cgtcgaacca ggcactgaag ctggcgctcc ggcaggagct tgcactgtgt
    29941 tatctccagc tcctgcagcc gcgcctagcg cagcgctact tggacggcaa cgacgaactg
    30001 tcccagcaga tccgcgccca gtccaatttg gtgtcggcac ggattctgtt ttccagtgcg
    30061 acgccaggaa ccatgccaag cttcccgtcg gtgatgtttg ttacccaggc ggtactgcgt
    30121 ggggggccgg cggtgctgcc ttcgcagcgg gccgaagcca agctgtggca ggtcgtcggg
    30181 ctggctggaa atggtcagat tgatgtggcg gtgaagctga tcgccgaagt cgccagcctg
    30241 tttgatgtgc caacgcttga actggtcatg ggccgcggcg gtctgccaca tttgcaagcc
    30301 cggctggcac tgcggattgg cgactattac ggcggcgcgc agctggctca gcaggcgatg
    30361 ccatcgctgt cccccgatca ggtcaaggcg ctgcatgtca gcatcaccac cgccgacaac
    30421 aacaaggcgg tcgatccggt gggcaaaggc gacctcgagc gggcaaatgg actgctgcgg
    30481 gcagcggtcc agcaagccca agccggcaat tcgccccatc tgctcaaagt gctctacaac
    30541 cttgccgtgg ttcagcttca gcgcggtcgt catgatgagg ccaagagcac cctggccaaa
    30601 gtcgatccgg tgcagcttcc cgaagcactg gtcgcgcaag gggcactcgc agaagctgtg
    30661 ggcgacccac gaggggcgct cgaacagtac cgacgctacc tgtcagcggg tggtgccgcc
    30721 aatccgccgg gcgaacagct ggcgagcgtg cgtgattggg tcgatgccat ggcgcgcttc
    30781 tacgaagggg ccgcgccgca tgccgagcca gcgtccgacg catcccccaa accagtcaaa
    30841 aagcctgttg cggcaccgcg gaggcagcca tgaagcgctg gttccgcggc ttgctggcct
    30901 tcgccatcct gttttggtcg ctgaccatcc aggccgcagc cccgtctgaa aaggtgctcg
    30961 cgatctatct tccgggggtc tacttcgcgc agctagagcg caagctcgat ctgggcagtg
    31021 agctggtggc ccatctgctc aagcgacttg gcgacaagta ccacctaagt ccgcgcgtct
    31081 atgtctcggc ggaagcgatg gatgccgatg cggcgcgaat tgccatggtg ctcaccgagt
    31141 caccgtatgt cgccgcgaac ctgaccaaga ttttgccggt cgcggtcgcg gcggcaggca
    31201 atggcaccga aaccagaatg gcactgctgg cgacaccgac gatccgcagc ctgcccgatc
    31261 tcaagcgcgg tggccttgtg tttgcgtcgt cactcgaacc ggcgcagtcg ttcctcgaca
    31321 acttcgtgtt cgaaggggaa cttacgctgt cgcgcgacat gatgagcccg gtgcgagacg
    31381 tagcatcggc gctgagcctt ctgtctgtgc agaaagccca ggcggtgctt ctgtacgaag
    31441 acacgattgc gacggtacgg accgccaacc tgcgcacgct gtatcgcagt gatcaattgc
    31501 cacgtccgac gctggtactg acatcccgca gcaccgaccc tgcggacgtg caaaagctgc
    31561 gcgaggcact gacttcgttc catggtcagg tgcatcccga catcaaggca tttcgggcga
    31621 cgacggatca gccctaccaa aagctgcgcg acaggatgca gcagaagccc cgtcgcaatc
    31681 cgcagctgct gctgatcgaa gacgacacaa gcccactgcc cacgccgcgc accaacttgc
    31741 cgtccccagt gcaggtgcca ctgcgggcat ttgcgcccga aggctagcta ctcctctcgg
    31801 agcttcagct ctttcatctt gatttgcagg ctcttgcggc tgattcccag gtggcgtgcg
    31861 gcttgggtga cgttgccttg ggtctcgctg agcgcccgct gaatcatctc gcgctcgacc
    31921 cgctcggttt cctgccgaac gatttccttt agcgaggtgg ccggcggacg acttgacggg
    31981 gtctgcggct ggctcatccg aggaggcggg ggcagctgcg ctccggcggt ctgcgccaca
    32041 ttcggtggca agtcgcgcag ctcgatgacg ggtccttccg caagcacgac ggttcgctcg
    32101 atgatgttct ctagctcgcg gatgttgcca gggaacgggt agcgcaacag cgccgccatc
    32161 gcgtcgtcgc ttagcccaga cacggtcttg cgcagccgtt cgttgaagcg tgacacaaag
    32221 tgcgacacca aaagcgggat gtcttgcagc cgctctcgca gcggcggcag ctgaattggc
    32281 acgacgttca gtcggtaata cagctcttca cggaagttgc cctgcgccgc ttctttcacc
    32341 aggtcgcggt tggtggccgc gatgacccgc acgtcgaccc gcagcgtctt tatgccaccg
    32401 acccgctcga actccgattc ttggagcacg cgcagcagct tgacctgcat ctccaccgcc
    32461 agctcgccga tctcatcgag aaacagagtc ccccgatcgg ccagctcgaa ccgacccggc
    32521 ttcgagtgga cagccccggt aaaagcgcct ttctcatagc caaacagctc ggattccatc
    32581 agcgtcttgg gaatcgctgc gcagttgatg cggatgaatg actcgttttt tcgactcgag
    32641 tgctcatgca gcgcccgcgc caccagctct ttgccggttc cgctttcgcc aatgatgagt
    32701 acggtcgatg gcgtatcagc caccttatca atcagcgaga agactttttg cagtgccggg
    32761 gccgttccga ccagtccata gcgaccaagc tgtggcggaa tctggccttg tggttttcgc
    32821 ggcgcgcgca gatccagctc gtagctggcc accgctttgc gtaccacctg caagacgtgc
    32881 tcgttgtcgg tccctttttc gacaaaatcg aacgcgccca gcttcacggc ctctaccgat
    32941 tcggtgatgc gaccgtaggc ggtaaagatg atgaccttca ggtcggggta ctcgctgatg
    33001 gcacgccgga aaagctgcat gccgtcgagc ttcggcatgc gcagatcggt gatcagcacg
    33061 gcgacatcac tgtttaagag cgcgagcgcc tcttcaccat cacgggcttc ctgcacgtca
    33121 tagccttcac ggcgaagcag agacgacaag accttgcgca aactgggttc atcatcggca
    33181 atgacgacgc ggcgagggga tgcaggcacg gcgcgaccct agcagagtgg ggaagggggc
    33241 gtcaaacgcg acgagattgc aggacgtcac ctacggcggc cagcgtgctg tcatcggctg
    33301 cgccttttcg ataggccaca tcgagagaag actccgcgat gatgcggtag acatcggcga
    33361 cctggcagct gacccggctt attgcgtcaa gatgtgcagc gactgtcatg gcatccccgc
    33421 gggcgatagg acccgtcagc gcctggggca gaccgacttc ttcgatgttg cgcagcacgg
    33481 actgggtcag atccgtgagc gctcgctgac cgacctgccg cggcagaccg acccgagcca
    33541 gcagttgaga cgcgacatcc agcagggtcg ctacgtggcc tgccgccatg accgcagcgg
    33601 cgtggtagat ggggagccga tcctcgctga gcaaaagggg cgtccccccg agcgcggcac
    33661 acagttcctg ccccgcgagc attccggcac cgtctccggc aatcgccatc acagtaccgc
    33721 gcagctcctg ttcgctgtgc ggaccacttc cgtcgctgtc atcgcttttg tcgcggtgca
    33781 caaaggaacg cagcggatgc aaaagcccaa tccctctgac cttgccgatg atgctcgcca
    33841 gcacttggga cgggggccga ctgccggcac agtgcagcac gaccgagctt gccccggcgg
    33901 cgccatggct ggtgatgtat tcggctgctt ggcttacggc atcatcccgc acggccagaa
    33961 tcacgatgtc ggcgcactgc tgggccgtcc cctcggggaa tagctgtccg tgagagcggg
    34021 accaggttcg caccggatag ccggcatgct ggagcgctct ctcgaacgtc tggcctacgg
    34081 gtccggcccc aacgataagg acgcgcgtgg tgcgctcggg cggcgatgcg ggttcatcgg
    34141 tgaacaaaga tgcggtcacg ctcgttcctc ctcgctggcc tgatacagcg cgtgctgttc
    34201 gatgtaatcg agtacgcggc gacaaaccca gccggtgggt cggccacctt tggacagggc
    34261 agcccggcag cccgtcgagc tcacttcggg caggcgtagc acttcatgat ggctgatgtc
    34321 accgctcgaa accggcacca cagcatcgct gtcactgccg ctgcggtgca gcaccagaaa
    34381 gggaagcagt ttggcaagtt ctggaaaacc gaaccagcgc tcacgctctt tgagcagatc
    34441 cgagccgatt accatggaaa actcgacgct ggggtgcttt tgcataagtc gccgcaccat
    34501 gtgcagcgtg tagcttgggc cgcccagctc ttcttcgatg gttgagacgt ggacccgcgg
    34561 acccagcgaa tcggccaaga gctggcacat ggccagccga tgccggaacg gtgtcagtcg
    34621 tttgtcaaag acatggcgaa acacggggac aagccacagc tcatccaccg gaaatgtctc
    34681 aagcacatag agagcggcaa gctgatgacc aacgtgggga gggtcaaagc tgcccccaaa
    34741 aagcgctacg cgcatacgtc catggtgagc ttccgcttcc atgaaatcaa ccgccggtcg
    34801 accgcatggc ttcaatcgat ttaaagaccc cgtaccctgc tcggtcaggc acttcggatc
    34861 ggctgggcga tcagtcgggc agggctgcgg tttcttccag ttctttgccg cgcgtttctg
    34921 cgactgccaa aagaatcagc agcatcgcga aaagaggtcc aatagccgtg ctctgcaccg
    34981 ccggcccata accaaaccgg acggcgagga agccaatcag cagcggagac agaacgccgg
    35041 cagttcgtcc gatcaggttg ttggccacgc caaaactctc ggcccggatt tcggtcggga
    35101 aaagctctgt cgtcagcgtc tgcaccaccg atagcatgca ggtcgtcccg gcgattccca
    35161 gcagcacgcc cagcgtcagc agcatctgtg acgagcgcgt gtaacttagc aagacgccca
    35221 ggatggtcag gccaaagacc accactgccg aagctttcct gccgacaacg tcgagcagct
    35281 tgccgacaga aaataccagc ggcagcgaac cgactgcggc gatggtcatg cactgtgcga
    35341 cctgggccgc agaaaagtgc cgctcggtga ctgcgaagtc tttccaaaac aacaaggcgc
    35401 tttgggtgca aaacatcaca agcgcccaga tgagggacag cagcaagagg cgcggcagat
    35461 agcgactctg aaacacacgg gccagtggca gtgccgtctg cgtctcggtg gcggcaaagc
    35521 gcgcggtgtc ccgcaggttc cggcgcagat aggcgacgag caaaagcggt acgccaccga
    35581 ccaaatagac catgcgccag ccgagcgtcg tggccgtgag caggggcgta acccccgcgc
    35641 agacgatggc cccaagcgag cttgtgcctt gaatgactcc gagcacgaag ccgcggcggt
    35701 cagcggggaa ttcttcggca gcaaagacca tcacggtggc cacctctgcc gattgaaaca
    35761 gccgcgccag catctggcac accgcaaact gaccgacgtt ttgcgacagt gccgacagcg
    35821 cggtaaagac catgtagccc gtgatggtcc acgcaagcag ccgatgccgg cccagtcggt
    35881 cgccttgccg taccaggaag aacgacaaga ccattccgat gttgatgaag ctggccagac
    35941 tggtgccttc gggcaccgtc agcgaaaatg ccgtgcgtag ctcgggaaga agctgggcca
    36001 gcgcgtagta gtcgaagccc tcgaagaagg acgcgacgga caaaaacaga aacagccgcc
    36061 tatggtacgg cgcaagtgcg ggcatcaggg cctacttggc tttcgccaca gcctggcaac
    36121 taccgcacgt cccgtacaag tagagccgcg cttcttcgac ttgaaagccg gccagctccg
    36181 ttggaaccac catggtcggc aagagacctt gttccagaca aaccaggtgg ccgcagcgac
    36241 ggcattctaa gtgcgggtga cccacccggc agccttcgca agcgtggacc tcgaagcgtc
    36301 gaccccgttc cgtcgtcccc accgcctgag caatgcccgc atcgaccagt gtgttcagcg
    36361 tgcgatacac ggtgacgtga tcgatgccag ctggtgccag cttgtcgatc acctcttgcg
    36421 cggacatggg ccggtggctg tcgataagcg ctgtcagcac cgagagtcga ggcgccgtga
    36481 tgcgcaggcc tcctttgcca agcagagagc gggcctgttc cttgtgattg tggctcgcat
    36541 gctcacccgt aggcttgctg tggctgtggg ccggcgctgg gctctgcttc tcgctgctca
    36601 tggtcagtct gcgatcggct cgaagtcgaa cttgctggct ccgcagtcgg ggcaaatcca
    36661 ggtgtccggc agtgactcaa acggtgtgcc gggtgcgacg cccgaatccg gatcaccaac
    36721 ggcagggtca tagatgtgct cgcaaacgag acagcgatag cgcttgagct tttgagtgtt
    36781 tgccatgatg aaaagcgagt ataggcgcag tggtcctgtc ggcaaaggac tcctgtcgac
    36841 tcgccccatg aacggtgaaa atccgatgca aatcaggaag aaagtcgtcg gtctcgggta
    36901 aggacccaag gccagcagca tgatgagttg atcaggcggg gtctttaccc aaggctggcg
    36961 actcgatgag tcgattaggc ggggtcttta cccaaggctg gcaactcgat gagtcgatta
    37021 ggtggggtct ttacccaagg ctggcgactc gatgagtcga tcaggcgggg tctttacc
//
LOCUS       DAORGN010000022        35390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_444_length_35390_cov_6.274770, whole genome shotgun sequence.
ACCESSION   DAORGN010000022 DAORGN010000000
VERSION     DAORGN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 35390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35390
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_35390_cov_6.274770"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PKL17_04355"
     CDS             <1..766
                     /locus_tag="PKL17_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43991.1"
                     /translation="AAEKEAGGVRLGDDEESAPAKSTASDPAVDELIAKYAGGGAATG
                     QRAASRPPAPPVPAAATGTRPATRPPRPPVAPTAAEAPAVAAPQSPAASAEPLPEVKV
                     DFPSVLRKAGLTDEEQSRVDKTLSLLAALPADTPLDIKRQIVGASLQAFGIPIEQIIE
                     AAVLQIRAFDKHSLEGQKQTLAFLEQGNRRIAELEKELSRIRSSMQEQQSLQKGLELT
                     CNQRKRRVQDVLDFFGPDAVDKATKVSAKLRQNQAT"
     gene            836..1534
                     /locus_tag="PKL17_04360"
     CDS             836..1534
                     /locus_tag="PKL17_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNK43992.1"
                     /translation="MSVFGRLANLWKGFISLWISDVEKDHPEIAYENAINSMVEKYSA
                     LKRATAAIIRRREELAERLTSQQKELAQVTSDLNTAIETNQDDLSVVLIQKKNQLESA
                     IAELQADSDLAAKDADSAKQSLLSVQNEIKKLKAEKETMVAKLESAQARMRIQEQLEG
                     LSVDAEVQALDNVRTHIKTQVAQANLNKELAETDLDSRLKSLRTQSGDVTAKQQLAEM
                     KAAAAAKRAAAKSM"
     gene            1590..3260
                     /locus_tag="PKL17_04365"
     CDS             1590..3260
                     /locus_tag="PKL17_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNK43993.1"
                     /translation="MKLTPFAKLFITVIILGVVGYVLYVKRDTITQWANQGGAKGVVP
                     AGDKPSSDQPSTGTGSGGISKDDFAAIGAAREAGRQGVTGISKAQLGSGKLARKLRVG
                     INTWAGHAPGIVSNGGLTSDKASVYLSKYGLDVEFTLLEDPQAKLAAFIKGDIDIMWD
                     TVDSWAREASALAEQGKGGKAIIQQDWSRGGDGIVALSSIKSIEDLKGKKIATTRYTP
                     SHWLLLYLLSQSGLSAADKSQIEKNLVFTAEAPLAAAAFKSKNVDAAVTWEPDLSGAV
                     KARGDEAAILVSTTAATNVIADVLVAQSELVEKSPETVTAFIHGWFDGIDAIGKDPTH
                     AYMLVGKALKLPEDDVSGMLSGMKLTSFADNALFFGLDGNKAQFEALFNAAFIVWRKQ
                     GVISKSVDAKNCVDTRFVSALADFYPGQKVVETFKFDPKKTDPAKERAIINKQLTIYF
                     TTGSDQIMAGSHFVLDSLGETMTAFGNTLLRIEGNTDNTGSRTANRSLSQKRADAVKD
                     YLIENHKVDPKRFQTVGNGPDNPVAPNTTDAGRQQNRRTDIKVILNVQ"
     gene            3263..4156
                     /locus_tag="PKL17_04370"
     CDS             3263..4156
                     /locus_tag="PKL17_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK43994.1"
                     /translation="MAQPSSPKKDVPRSPLRSREPIWTLRKPLPTSWQFWLGLLLPVL
                     LLILWVSLTSGAKPVVGGAFLPKPTDVLRVLLRDLIIGKQDVIDGQPVVVRVLLDAAI
                     ASSWRIVLSFLLAAGVALPLGILMGAFEPVNRIVEPVMAPLRYMPISAFIPLTILWFG
                     IEEKQKIAFLFLGVFVYLLPVVVSAIRAVPEELVQTARTLGAGRFQVIFTVLVPAAAP
                     DIFDSFRVMNAISWTYVILAEFVNTRRGIGYQIQLAGNHLHIDEVFAGILIIGVIGLL
                     SDAMIRFCAKLLFPWREGGHV"
     gene            4149..4967
                     /locus_tag="PKL17_04375"
     CDS             4149..4967
                     /locus_tag="PKL17_04375"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK43995.1"
                     /translation="MSEPQAAQIPVTVHSTAVKLHVEDLHVTYRGRGGEDVEAVRGVS
                     FDIVDKPGIGEIVVFLGPSGCGKSTILKAVAGLLPPTKGRVLIADHEVDGPGRDRGMV
                     FQQYTSFSWLTVVENVEYGLRLQGVPSQERRSRAMEILKHVGLDQFASKYPKELSGGM
                     KQRVAIARTLINKPKLLLMDEPFGALDPQTRWEMQGMLIDISHREDNTILFVTHDVAE
                     AVYLADTVYVLSKRPAEILHRIDVPTFSDRTHKVKASPEFRQCEEQLLSLLYAK"
     gene            4964..5773
                     /locus_tag="PKL17_04380"
     CDS             4964..5773
                     /locus_tag="PKL17_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43996.1"
                     /translation="MSERDTSARGKPSEPPYAKYAFANPYNLSLLVGAGSAALLTGHW
                     WVGLCAVAAEAVWMLFAPDSRLLQKKWFDPIWEGEQQEARKARQAAKFNALPDAEKER
                     ALRLRDQQKRIEKMAQDNASFSVDLLSSDIAKLEDLVDDFLDMAQVASRYEAYLKDMN
                     LVEIEKDITRYSNQVDKLPVGDERRKVAQKNLEVLLSRKDRYLELRRNLQTARGQMDL
                     MENTFRLLSDEIVGMQNPSELGARLDELREGVAAVRETSRETERYMQGTTR"
     gene            5770..7653
                     /locus_tag="PKL17_04385"
     CDS             5770..7653
                     /locus_tag="PKL17_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK43997.1"
                     /translation="MTTLSTSKTTRLPVWADDIRRRYLRGESTQFVLFGNVHDLILDD
                     PDGQIDKRSADERLLPLPEFLSKVLLEKKSMVILYNVSTGVRFVRGKLPAGFEDLALQ
                     KEPSKILPLLERLLITQNGVAVVIEYAETVAPAGETSFSTIEDRSSVVTLQRWSMLPA
                     LDKNDSLVFMLVENLSELHPKLVANPRVATVQIPIPEAEARQALLRHIRPDMPQDELE
                     MMTEVTAGLKCIQIQGILAPHDVPSDDEAERTKYIAELLGGGAAAMERAQKLAQITKG
                     MPRSEIRRLIAPDGPVTTHADTDARAEVLRLIAARKREIIERECFSLIEFVEPKHGFE
                     VVGGMDEVKKDLSLIAKNIREGRRSRVPMGLLFVGPMGTGKTFVAEAFAKESGLTAIK
                     FKNFRSKWVGATEGNLERILQVVQAIGQVMVIIDEADRAFGNQGEDDGGTSSRVIARI
                     KEFMSDTANRGRVLFVLMTNRPDKIDIDLKRAGRLDRKIPFFYAQTVAEVEPILKAQL
                     RKNKVQSELAFPIAGDALARIVGMSNADIEAVVLLASDYASQRAETLGTGEVPVSIDD
                     LQRAARDYLPPRDLEMLEFMELLAVFEASNRRMLPPKYADLPIEALQERLQRLKVVAG
                     HRR"
     gene            7724..8167
                     /locus_tag="PKL17_04390"
     CDS             7724..8167
                     /locus_tag="PKL17_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43998.1"
                     /translation="MNFFPEIQLTQAQAEAIARGLFHIAHSDGLHEREAALVASFWAE
                     AGGSHNALSDLARRGPLSTEELTAVLDTEDLRKLFLKTALLLAFADGQVSGAESELVR
                     KYAADLGLGESLGTLETQVKEYLLLQLSHIHNTQALAQIAKKLAI"
     gene            complement(8203..8547)
                     /locus_tag="PKL17_04395"
     CDS             complement(8203..8547)
                     /locus_tag="PKL17_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK43999.1"
                     /translation="MLTKSRRRFLIELPLAAAVLLTAKNALAEHYPASLCGDCSFGSK
                     KDNCAKCGKWMGSTRIPARLCGDHGFGSKKDNCVKCGKWVGSSKARAFVCGDCGFGSK
                     KDNCVICSKWAP"
     gene            8684..8989
                     /locus_tag="PKL17_04400"
     CDS             8684..8989
                     /locus_tag="PKL17_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44000.1"
                     /translation="MAYFCHSCGNEQIFEVKVGIKVGRRDTCPHCGTDLHCCKNCQHY
                     DPGLHNQCREPEAAFIRDRTEANFCPHFGMRDSEGWKEDRSAEDAKKKLAALFKNLK"
     gene            8986..9336
                     /locus_tag="PKL17_04405"
     CDS             8986..9336
                     /locus_tag="PKL17_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44001.1"
                     /translation="MSIRLAEEDAPFDPESRRLCGDPACIGVIGDDGHCKECGRIGEG
                     EEPHARHFSEDEGQTSADDVAAAPTETESAAPAQSEPASDAGFEDRRLCGDPGCIGVL
                     SADGRCSECGRVFA"
     gene            9432..9941
                     /locus_tag="PKL17_04410"
     CDS             9432..9941
                     /locus_tag="PKL17_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44002.1"
                     /translation="MSRMLGHIAVLSLAVQIGCLIPIPLEQQVTTDGGRSLQVKAAIP
                     PFGTQRATSILEKYAYQVDVVSDSADVAARLYLQINGTCCDLNIDNPNVTRWSQQAQA
                     QAVDSSTGSYSMVFSQPVLPCTMVPAGAMVYVVPVIASGGFADFQMGVRPDGLGEVDR
                     SHYWTVICP"
     gene            9977..11776
                     /locus_tag="PKL17_04415"
     CDS             9977..11776
                     /locus_tag="PKL17_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK44003.1"
                     /translation="MPKPTSYLLRLDGGPKRRDRDLWAPFVLALVASLAACGEEAASV
                     PIACSGDLTCPPARNSAAWAVQLWPTDSGDKIGADQHFLLTPQEGQQLNFDGTGSATL
                     RFRAASLIGGLVLTADNRALIHARVQATLPSAIPGQNDYRFDTQTAEQVAGTYSLRVP
                     TPAKPMDQLYRLWVGFDDAMQAAMYPPMWIEHPVSSDVELPIQLRPANRLAVVSGRIV
                     NPLGEGVGGMTVQVFDENNQIISSSAVSLTGVGTSAGTYRTVLDPSLASKSTVNLRVV
                     VRPGTTQPGLPSLEVLIPPPTAGTERTQNFVLPSYRTPVPFMLPVRGLGPSGATQEVP
                     SARVVAQVHLEDATTLSGVRVIYTAQADTDAQGIAKLQLVPAPSGGSNLTYRVSIVSP
                     SRLPYASVSRDMQVGPTDGLLSALTLPTRSRLIGRLLSASGEPVSGGQVVAQPIAKPV
                     EVRSPLDVVPDLTLPQTTTDSDGRFVLRLDPGDYDLDMIPVLGTGPRTSLDNQRIRDV
                     DVDLGEVRLPRLALAKVLVVGPSLAPAPQVKVRVLELPDTSPRYGLACTLDLPCSKVA
                     KVRAEAFTDSKGRVQFLLPDSLPSASLLPHSLP"
     gene            complement(11738..14590)
                     /locus_tag="PKL17_04420"
     CDS             complement(11738..14590)
                     /locus_tag="PKL17_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK44004.1"
                     /translation="MRIVGRLATTGLLLVPLFACVPKPISVAALVDPKPLLPSQPHVE
                     TVEPPPNTQNLLSAPPPEVTPSAPLWKSLPNGVRIQLLPDRLVGTVAVQLWVSGGVAS
                     EPASGLGLSHLTERLLLHELAKSASDNIRAAGGDVSSFTTLDHTVFQLLLPKGQQALA
                     LDFLAAVLMPTPTVDDAMVARQVAQVEDEHKRAQLMLRSVAVEKLRELSFPGHPYARP
                     LLGDRLRLSALRSDDVLAYYRQTYQPAQLSLLVSGALADTLTHPLTSLLSGLAAPQTA
                     ATQPTTQTLTRPHSPQAWVASHDDPSGQAVVAFALPGVACAEEEVAAFDVFAQLVRSS
                     LGFRSLGSRPKSEPAALSFSGRQSGLFFAHMMVPPSQVEEMSAKLLHELLRPSQRDLE
                     PGELARAKQQLLAENARLLDTPSGRARRAGYFTSLGLSEDRYNQAVRELSDASLRQIL
                     LRHLQRDSLIGLWLWPRSRTPKDDAAQLERSRNRLLAQLKTPPPTVQKPLAAARKDGG
                     VWRYQVPGGPLVLLLPDHSVPIVAAAATWPGGLSLEDEQTAGGHELISRLWKETPRAR
                     GGQWADTLAQLRGTVATQVERDAFSLVGEWPSQAEEEALPLFLDGLSAPEPSDAELEL
                     RRRQLVAELRQKDESRAVALAKLKAKLYGSHPYKLEPSVQSLSGLSRKRLGELFRRSY
                     GPSQMTLAIVGDIEPYAVFSLIESRLQNSGKTASPPPSPLSTIETPRQYLSYHPHEQA
                     ILGLGFRTPGLKGQDALALAVLSEILVGKAGRLHRELVERRGLLLSLDGKLEGGLQGG
                     ALLISAVTTPTSLDSAEASLRDELRRLLDHPLSDDELQQAKRQLLAQKWKARQHRSDV
                     ALSLARRSALDLPSQESEEEHISSLTAVQVQTVAQRYLDEQHAVLQAVLPQALLAAKR
                     GDARDAKAGPKLLAKLIPNVSKSGSGKAVRKQRSRR"
     gene            14716..16794
                     /locus_tag="PKL17_04425"
     CDS             14716..16794
                     /locus_tag="PKL17_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNK44005.1"
                     /translation="MNTLTSIPSSSSHTMPLTLLSSPLDAAMSHLRSLQKADGGFEGE
                     MVWNTMILSQYILTQRAIGKPIDKQTADKMVLHYRTTRCKDGAWGMHGESDGYVYFTA
                     MAYVALRVLGVSASDPLCADARAWLARQPEGVLGIPHWGKFWLAMCGLYGWDGVNPFP
                     PELFVTPKWLPIHPFRFYNHTRYIYLGIAYLYGKRFQISLGDLLPTLRSELYDQAGRR
                     PYGEIDFHSYRHVVAKTDLYVAPSLPLKAAYEALHAYEKRPIAALRQYALGFCLDRIL
                     YEMRASKYQCISPVNGLLNCLAIYSHNPQHPELASCLLGLEAWKWEDEAEGIRYCGAR
                     SNTWDTAFAILSLTEADRAQQLAPHNAETLRRAYAHLCSLQMQTELDGWEAEQREPIR
                     GGWCFSDGEHRWPVSDCTAEAVCALLSLHYEGSPVPESDRLPVRRFVEAAHFILRRQN
                     EDGGFGSYEARRGGSLLEKVNPSEMYGACMTERSYLECTSSCLRGLLALRNRLGAKLG
                     GSLLLEINTAIGAAVMLLRKSQRPDGSFPGFWGINFTYGIFHVVEALHEAGIPSTDPA
                     IQRAAVWLLGKQRPDGGFGEHYSSCLTGEYVAHSESQVVNTSWALLTLLLALPPSHEA
                     TQRGLDFLCKMQGKDGGFPQQAQAGVFFGTAMLDYRLYKSYFPLWALSRAARLGQELR
                     HARTSHPLTR"
     gene            16763..19126
                     /locus_tag="PKL17_04430"
     CDS             16763..19126
                     /locus_tag="PKL17_04430"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNK44006.1"
                     /translation="MPGPPIRSLADDHGIRATLFSVGRTRRKRTRIFLPIFLGLFSAA
                     LLFGLLVLRAWLLPRYNDKFGDAPLLGHLEVDRKLPAQTHRIGPFQLSFTPDSGGVLY
                     VVHEKEPERRIWETLPGMSFVAAAYGHEKVDESRGSFFFSDKATGSTCATQRLDQIAG
                     SNGIVKLSGTLRCSGAGSAPYVLVFSQVAQNQLGFELRIEDDDLNRAFLTYASPTTEH
                     VFGFGEQFTYFDLKGRRVPIWVQEQGLGRGLQPITTAVDLVARSGGSWHTSYASIPHY
                     ITSTARSLFTENYEYQIFDLRTPDRIQVRVYSRTMKGRILYGKTPPDLIREYTEYAGR
                     MRPLPDWIHQGAIVGIQGGTARVQALFDKLQAEGAPLSAVWLQDWVGQRKTSFGQQLY
                     WNWQLDRSRYPDWEAMRRKLAAQNVRVLTYVNPFFVDIAKLGRPGRNLFAEAHDRGYL
                     VREPNGQPYMIPNTDFSAGLLDLTHPEARKWWKDIIRSEVLATGASGWMADFGEALPY
                     DALLHSDVAARSFHNQYPEEWAQLNREAIREAGREDDVVFFTRSGYRKSPGSSTLFWL
                     GDQLVTWDVHDGLRSAVVGMLSGGVSGFSLNHSDAGGYTTIVNSILKVKRSRELLWRW
                     LELSALSPVMRTHEGNRPDDNFQIDGDAATLRHFARCAKLYKAWGDYRKQLVAQAAAT
                     GMPVARHLALHYPDDPNVYALSFEEYLLGEDLLVAPISEPGANQTKVYVPEGTWVHVF
                     TGQSVTAGKSGLRVDVEAPFGKPVMFYRQGAAMVAPLLKAMKEQGLL"
     gene            19237..22443
                     /locus_tag="PKL17_04435"
     CDS             19237..22443
                     /locus_tag="PKL17_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNK44007.1"
                     /translation="MKCKLAARSPDADKSSVTSRLTGTLRHCLAWVATLAVLGAASSA
                     SAAGPKGCVVYASTDSSVTGDIKSKLEGTKLFDSVTMIDGGYTAPADATLAKCDSVLV
                     FGDYNKGGFNDPTGVGDALARYIDKGGGVVVALPFYGGYYYNAPTGTNWAKYQLIDNS
                     SSMSYVSSKSLGSKDAHPVLSGISKVDTNGSTCRERSSVTSSTVKSGAKVVATWNDGN
                     GMVIAGFPNGRPRIDVNIYPVSSDIGYYGCYNSASDAAKLIAQSMIYVASPLRANPSP
                     ADFGEVAVLGSADLNVDFENQGSAPITIASGDITPTGVFTVDTVSFPKTLNPGDKLSL
                     KFSAKPNAAGQLSAIYTLTPSTAGVAPGVVQLTVKGIGPRYDAQPSQIDFGGVPVGMT
                     ASAVTVNVTNTGGGYLGFNTVSIDDTTNFALENVPMATLLGSGAGVSFDVKFKPTLEK
                     AYTTNVRVAYSISGVNYSGIVTVKASYGKPKIVAPASLVLSPVRVGATGPDQIITLQN
                     TGQADLSITNIQFTSGDMSDFAVVTAASAMSPFKINTASSGDIKVQCKPGVQGIRQAN
                     LELTSNDPSLAMGKTNIAVSCRGTVANFELSVDKIDFMGNQQAGQCSGAQYVTVKNSG
                     TDALKILSLAFTGTNASSFKHSAPATRTVPGSGGTFVIPVQFCPVDIGAMSAALEIAT
                     DFKPGHVAKVPLTGTSTGPKVVVTPGNIDFGTVYIKTTSTSKQIKITNDGDQDLVFGK
                     SVLTPAMGAFKVAGLPTEGKILKKGDPAVVLDVQAGPVTAMQQSGEISIIVNDQIKMN
                     NIRIPLSVTGVQADLKADPMMITFPLTIVGLRSQEQTVTVTNIGAAPLTGLDLSVSGT
                     ASSDYLVSGDRTVTIPPSGKAEFKVTFKPTGSGPRSAILVVNAAGLMTPTQVKLEGTG
                     KLLTVSCSPDDKNLGMVRIGESVSLKVICRNSDSSDIDFVTGFSDNPDDWSVDPAMDK
                     LPAGTPTEEGLATLNITFKPTVTGPRTTTLTLKTKEGLVIGSITLDGTGLPMPKEKMM
                     EDQGGCQAGGRPSSAAGTLFFALLSVGTLILRRRRAAL"
     gene            complement(22517..23413)
                     /locus_tag="PKL17_04440"
     CDS             complement(22517..23413)
                     /locus_tag="PKL17_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44008.1"
                     /translation="MKGHGLPALASAWLQNVRRLMVPRPPWRADAEFDYYRVDPAELG
                     AQLARQKAWQRQGHFVGLGVVQALGLLDRTRLAGKRVLEIGAGECMLSQALLVAGASE
                     VWAVDAVPKQLWAAAASQPADGPLHCVIADAMDLPFSDGSFDFVVANLVIHHIRPLSR
                     LLPELVRVLAPGGRLLALEPAPIVGLFVHDQTSDNEAPLWPQTVTQALADAGLLETAH
                     QYLWLRLQTTRFGPLSPSYRVSGARPGQMPAQLGLRRSLRPSALPGLHIDSGCTFAPL
                     VEQQLDEIVACGSREGLLKKAE"
     gene            complement(23410..24804)
                     /locus_tag="PKL17_04445"
     CDS             complement(23410..24804)
                     /locus_tag="PKL17_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNK44009.1"
                     /translation="MNDNSGTAKLPVVASTDVPHGLRPTVDPATLTHRQLRRDRFWSH
                     IPAYAEVDEQTFVDHKWQAKHTITNPDKLLAAVQSLVSPEFYKDVAAGFEQAPMSIRV
                     SPYLLSLIDWSNPYRDPLRIQFIPVRSRLMADHPKLGLDSLSERADAPTPGLTHRYVD
                     KALFLALDTCPVYCRFCTRSYAVGVDTKQVEKFTLRVSEERWAKAFAYIASRPELEDV
                     VISGGDAYNLRPQQIREIGDALLGIDHIRRMRFATKGPAVMPQKLLTDHEWVDALTYV
                     VEKGRKLGKDVMLHTHFNHPNEITGITQDAIQVLFGRGIFVRNQSVLIRGVNDSVATM
                     GQLLKRLGYISVHPYYVYIHDMVKGVEDMRTTLQTALDIEKQLRGSTAGFNTPTFVVD
                     APGGGGKRDAHSFEHYDRTTGVSVYSAPAVKPGQLFFYFDPIDRLPPEGQARWADPAE
                     HPKMLEEARQKVHL"
     gene            complement(25003..26280)
                     /gene="tgt"
                     /locus_tag="PKL17_04450"
     CDS             complement(25003..26280)
                     /gene="tgt"
                     /locus_tag="PKL17_04450"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNK44010.1"
                     /translation="MIPSHANRLVSQAEHELPATPPADGPIYQLDGQDGKARAGRLLL
                     PHGVVKTPIFMPVGTVGSVKAMTVPELTQLGAQIILGNTYHLWLRPGMDVISAHGDLH
                     RFTTWKRPMLTDSGGFQVFSLGAAMASGAATTQEAGSRPAVQGGLVKITDEGVRFRSH
                     LDGSLRLLTPEESLRVQATLGSDIAMAFDHCPPGLSPPAEAQAAMRRTTAWAARCLSV
                     PAPMLTDGKNPLGHQKRFGIVQGGIHVDLRRQHMAEICALPFDGFALGGFAVGEPIQT
                     MYEVLDIVACELPSSKPRYLMGVGTPRDLVHAIGSGIDMFDCVMPTRNARNGQLFTSL
                     GKVNIRNAKYQRDLSPLDPDCSCETCRSYTRSYLRHLFIAKEILFSRLATLHNLTYYL
                     NLVERARAAILAGEFKKFAEAECKRWDDRARTD"
     gene            complement(26293..27429)
                     /gene="queA"
                     /locus_tag="PKL17_04455"
     CDS             complement(26293..27429)
                     /gene="queA"
                     /locus_tag="PKL17_04455"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNK44011.1"
                     /translation="MTALSAADFDYELPDELIATRPAEQRDLSRLLLVERDTLSHHTF
                     SSLPDLIEQKIGPGTLLVVNDSKVLLARLYAKKRSPDGQLGGRVELLLLEPHVGDDHK
                     TSWRVMYRVSKPLRDGTTLQILDRHGEPLANRTLTVRCVEGEGRAIVDFPTDVALDEV
                     LSQAGELPLPPYIVRQRQLTGDIAQKEDDSRYQTVYAKVLGSVAAPTAGLHFTDTLLA
                     ELSRRGIEKHSVTLHVGPGTFLPLRDDDPQKHVMHAERYHIPESTSDAIARARREGRK
                     VLAIGTTVVRTLESATKEGESVPTPGMGQSQLFIYPQAPSGRYAFRVVDALLTNFHLP
                     RSTLLMLVAAFVGRERILAAYAQAIAARYRFYSYGDAMLLPGRM"
     gene            complement(27429..28904)
                     /locus_tag="PKL17_04460"
     CDS             complement(27429..28904)
                     /locus_tag="PKL17_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNK44012.1"
                     /translation="MRRHPVGDIAQLGGPVPTPLFRTKSISKVIAEAEGSGSRLQRSL
                     SAFDLTMIGIGAIIGAGIFALTGNAAATKAGPAVVLSFVLSGLGCAFAGLCYAEMAAM
                     IPVAGSAYTYAYATLGELFAWIIGWALILEYAVGAITVAISWSGYLVRFLEKGFHIQL
                     PFEWVNGYMVTQTDAAGIVHHGIFNIPAVILVGLITVLLVVGVKESARATSAIVFIKI
                     IAVLLFIGIGAMHINTANWSPFVPFGPWGIVQGAGTIFFAYIGFDAVTTMSEEAKNAQ
                     RDLPIGIIASLVVCTVLYILVAAVLTGMVPYLELKDPAPVTLALSRAGVSWGVMLVSI
                     GALAGLGSVVMVMMMGQPRVFFAMSRDGLLPASISQVHPKYQTPFRSTILTGAVVAAC
                     SSTMSTEVAGNLTSMGTLFAFVLVCLGVLVLRKSDPTRERPFKTPLVPVVPVLGMATC
                     LGMMAGLDKATWLNFFAWLAIGLVIYFSYGKRHSVLAKSPT"
     gene            complement(29000..29341)
                     /locus_tag="PKL17_04465"
     CDS             complement(29000..29341)
                     /locus_tag="PKL17_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44013.1"
                     /translation="MRAESPAKLVYFRRELPPLQDETAGEFVVEAESPPVPNTGLGRD
                     ELWTHCYDDLMHSAVERIGQEVVRLHGSCAHVVDEQIEPKVSYERGEAWLWGMFTFVL
                     YRHPGPSTPAS"
     gene            complement(29344..31422)
                     /locus_tag="PKL17_04470"
     CDS             complement(29344..31422)
                     /locus_tag="PKL17_04470"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44014.1"
                     /translation="MMSANPQPKSAPPIPLEAIPTGRPGAVHDRPSVNMYSMSPPGPG
                     GGGMGGGGGAAAGGTGGAVGAPPGGKYAILRVISGNDQGKTFELKHTSTTIGRGADQM
                     VIVADIAVSRKHLQIHMSGNGYRMQDMGSPNGSMVNGKRQAEGQLVDNDQIEIGNTTM
                     RFEHSPSRAQAEPVPQPMQQPMMQQPMMPQPMMQQPMMPQPMMQQPMMPQPMGSYAGQ
                     PPMGYPPSGYPQPGFAPQPGMPPALSPPTSAVASPPMMTGTPASLQSQSMSMGGEGAA
                     GGPLAFLSDSRKRTLYLGAIAGALVVGGIGLILTSALRSSGGGKALDAVMDQYVTGTK
                     NFTAGHYDDAKKAFQAAAEKAPDSTVLKRYIELCDSEAKMAAALDAAKRALDKRSYAD
                     ALKEFEKVPKDSTLYEDAQQQARLARREAIKALVSEANSLAKSDTSGALDRVRQGLEI
                     DPESSELLELKARLSGGGKTEETTQVAAAEPEKVEKVEKVEKPEPVKKAEPVKKAEPV
                     KKVEAAPAAAGGDLDTNKQAASSYKNRDFAGAISALSATKHPKAAATIKDIQDVKTQV
                     EKAAKSEGSSPKDAIAAYQAAQTADKRLGGGLSGFLGGKISGLQSKAGGGQTAAKPAA
                     GGDPAKDAQADQLLAAAKSMVAKNPGQARNNCRKVMQMFGNNPSHPKVKAAYTLLQSI
                     KGGKDDDDDF"
     gene            complement(31529..31825)
                     /gene="xseB"
                     /locus_tag="PKL17_04475"
     CDS             complement(31529..31825)
                     /gene="xseB"
                     /locus_tag="PKL17_04475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNK44015.1"
                     /translation="MAEEQQTVTPPDERELGFDQVLDRLHKIVTRIEQGNLPLEQALS
                     LFEDGVRLSRRGSAILDATEQRIELLVKNDDGSESRRPLAAPGASLGGSPADST"
     gene            complement(31901..32254)
                     /locus_tag="PKL17_04480"
     CDS             complement(31901..32254)
                     /locus_tag="PKL17_04480"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK44016.1"
                     /translation="MLKLTRSIPGLFAFSLVVLAGCHGYKDTGIATVRQNNNDLKSCL
                     QEASARNPNLKGMMELAFEIGPDGKVNRFGFAKDEYKDQMLSDCVKAKATTWQFPAPP
                     SGKMEQFTYKFGSAN"
     gene            complement(32289..33503)
                     /locus_tag="PKL17_04485"
     CDS             complement(32289..33503)
                     /locus_tag="PKL17_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK44017.1"
                     /translation="MNRPAEPIPKPRLRVLHVVPSFYPAVGYGGPVRALFELCRAQVE
                     AGLDVRVLTSDADGTQRMTALSNRWVTDIGVPTFYAPVRIGKDIAPSLVVRIAQALAA
                     TDLVHVSGLWSPTSLLGLSLAALHRRPIVLSPHGSLMPWALHSGRGRTQKMAALRLLR
                     PLIDRIGGWHVSSQAEADGIRALCSDGLLSARMPIACVEYGIWPDAIATTDKPPLARN
                     PQIVVLGRIHPVKRLELAIDALWELHKTHPTAQLILAGPTEDPGYRARLEQQAASRGL
                     AQAVFWPGLLDETKKTELLSSAAVLWLCSHMESFGLVVIEALSQGTPVIATTQTPWPQ
                     LASERIGAQVDADPRAFAESTRALFSQPEALQKAQSNRCRDFVRSRYTWPALTARLCA
                     FYETCAGVTLHP"
     gene            33695..34462
                     /locus_tag="PKL17_04490"
     CDS             33695..34462
                     /locus_tag="PKL17_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44018.1"
                     /translation="MTSLPGGLRCEDLIAFDVVPSLLSAAQSAGLSGLLPLQEQAIRA
                     GLLSLPDGSRPEQDLLFIAEPGSGKRTIAELAAAHHAHAGHRVLLVTHDLESARRCVQ
                     RFAAYAAIGLRTGLLHDPADFAALDLAVAPLDTAALLLASDEPVRRGLGLVLVEELEQ
                     VADSADSELWPLLLLRCQALRADLPLRMLLLCGDVRLGEKLALLLRAKVVRAGRQLPS
                     EEDAPSLKRARALLQLRGGIAARRDNTCTAVHVARPR"
     gene            34549..>35390
                     /locus_tag="PKL17_04495"
     CDS             34549..>35390
                     /locus_tag="PKL17_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNK44019.1"
                     /translation="MNAASPVATASPSPTSLVTSTDQLVEHFRRGVKTGPLRIGLEHE
                     KIGVALDEDGTVRPVPYADKPGRPQIRALFAGLCESCGCEAVLEGGNTIALKRKDGAI
                     TLEPGGQFELSARPFVLDTECAADLDEHLRELLPLAERLSIAFVGVGFRPLGTWDDVP
                     WMPKGRYKVMREYLPTRGKLGVEMMKRTATVQANLDYVSEEDAILKMRVGVGLGPLVT
                     ALYAASPLVDGKPTDYKSFRAHCWLDTDNDRCGVLPFLFRDGASFRDYAEWALDVPMF
                     FVYR"
BASE COUNT         6833 a        10609 c        11073 g         6875 t
ORIGIN      
        1 ggcggctgaa aaggaagcgg gcggcgtgcg gctcggcgac gacgaggaat cggctcccgc
       61 gaagtcgaca gccagcgatc ctgcggtcga cgagctgatc gccaagtatg ccggtggcgg
      121 cgcagcgacg ggacagcgcg cggcaagtcg acccccggca cctcctgttc cggctgcggc
      181 gactgggaca cgtcctgcga cccgtccccc gcggcctccg gttgcgccaa ctgcggcgga
      241 agccccggcg gttgcggctc cacaaagtcc ggcggcgtcg gcagaaccgc ttcccgaggt
      301 caaagtcgac ttcccatcgg tgctgcgcaa ggccggtctt accgacgaag aacagagccg
      361 ggtcgacaaa accctgagcc ttctggccgc gctgcccgcg gatacgccgc ttgatatcaa
      421 acggcaaatc gtcggtgcgt cgctgcaagc gtttgggatt cccatcgaac aaatcatcga
      481 agccgcggtg ctacagatcc gtgcgtttga caagcacagc ctcgaagggc aaaagcaaac
      541 gctggcgttt ctggaacaag gcaaccgtcg gattgccgag ctggaaaagg agctttcgcg
      601 gattcgctcg tcgatgcaag agcagcagtc gctgcaaaaa gggctggagc ttacctgcaa
      661 ccagcgcaag cgtcgcgtgc aggatgtgct cgattttttc gggccggatg ccgtcgacaa
      721 agccaccaaa gtttcggcca agctgcggca aaaccaagca acgtaggctt cggccaagca
      781 aaatagccag atcatcacgc cgccctttgg cggccacact tcaaggagca cgcggatgtc
      841 agtctttggt cgcctcgcca acctgtggaa gggttttatc agcctgtgga tttccgacgt
      901 cgagaaggat caccccgaga ttgcgtatga aaacgcgatc aactcgatgg ttgagaagta
      961 ctcggcactg aagcgggcga cggcggcgat cattcgtcgt cgtgaagagc tggccgagcg
     1021 gctcaccagc cagcagaaag agctggcgca ggtgacttcg gacctcaaca ccgcgatcga
     1081 gaccaaccag gatgatctgt cggtggtgct gattcagaag aaaaaccagc tcgaaagcgc
     1141 gattgccgag ctacaggccg actccgatct ggccgccaag gatgccgaca gtgccaagca
     1201 gtcgctgttg tcggtgcaaa acgaaatcaa gaagctgaag gccgaaaaag agacgatggt
     1261 tgccaagctg gagtcggccc aggcgcggat gcgcattcag gaacagcttg agggtctgtc
     1321 tgtcgatgct gaagtgcagg cgctcgacaa cgtgcggacg cacatcaaaa cacaggtcgc
     1381 gcaagccaac ctcaacaaag agctggccga gacggacctg gattcgcggc tcaagtcgct
     1441 gcgcactcag tcgggcgatg tgacggcgaa gcagcagctc gccgaaatga aggccgccgc
     1501 cgctgccaag cgcgccgccg ccaagtcgat gtaacccacc gctttggaaa cgtcaccatc
     1561 gccttcacga cgaaggcgcg gagctttcga tgaaactcac cccttttgcc aagctattta
     1621 tcaccgtcat catcctcggg gtggtcggct atgtgctgta cgtgaagcgc gacacaatca
     1681 cccagtgggc caaccagggt ggcgcaaagg gtgtcgttcc cgctggtgac aagccaagtt
     1741 ctgaccagcc gtcgacggga actggatcag gtggtatctc gaaagacgac tttgcggcga
     1801 tcggtgcagc gcgcgaggcc ggtcggcagg gcgtgacggg cattagcaag gcgcagctcg
     1861 gcagtggcaa gctggcccgc aagctgcgcg tcggcatcaa cacctgggcg ggtcatgcac
     1921 cggggatcgt ttccaatggc ggtctgacga gcgacaaagc gtcggtctat ctgtcgaaat
     1981 acggtctcga cgtcgagttc acccttctcg aagatccgca ggccaagctg gccgccttca
     2041 tcaagggcga catcgacatc atgtgggaca cggtggattc gtgggcccgc gaagcgtcgg
     2101 cgctcgctga acagggcaag ggtggcaaag cgatcattca gcaagactgg tcgcgtggcg
     2161 gcgacggcat tgtcgcgcta agcagcatca agagcatcga ggatctgaag ggcaaaaaga
     2221 ttgcgacgac ccgctacacg ccgtcgcact ggctgcttct gtatcttctt tcgcagtcgg
     2281 gcctgtcggc ggcggacaag tcgcagatcg aaaagaacct ggtctttact gccgaagcac
     2341 cgctcgcagc ggcagcgttt aagtcgaaaa atgtcgacgc cgcagtgacg tgggaacccg
     2401 acctgtcagg tgcagtcaag gcgcgcggcg acgaagcggc catcttggtg tcgaccaccg
     2461 cagcgacgaa cgtgattgcc gacgtgctgg tggcgcaaag cgagctggtt gaaaagtcgc
     2521 ccgaaacggt gacggccttt atccacggct ggttcgacgg cattgatgcg attggcaaag
     2581 atccgaccca cgcttacatg ctcgtcggca aagcgctcaa actgccagaa gacgacgtgt
     2641 cggggatgct gtcggggatg aagctgacca gctttgccga caacgcgctg ttcttcggtc
     2701 tcgacggcaa caaagcgcag ttcgaggcgc tgttcaacgc cgcgttcatc gtctggcgca
     2761 agcagggcgt gatcagcaag tcggtcgatg ccaaaaactg cgtcgatacg cgcttcgtct
     2821 cggcgctggc tgatttttat cccggtcaga aagtcgtcga gacgttcaag ttcgatccca
     2881 agaagaccga cccagccaaa gagcgagcca tcatcaacaa gcagctcacc atctacttta
     2941 cgacgggcag cgaccagatc atggctggct cgcacttcgt ccttgattcg ctcggcgaga
     3001 cgatgacggc atttggcaac accctgctgc gcatcgaggg caacaccgac aacaccggat
     3061 cgcgcacggc caaccgctcg ctttcacaaa agcgcgccga cgcggtgaag gactacctga
     3121 tcgaaaacca caaggtcgat ccgaagcgct tccagaccgt cggcaatggt cccgacaacc
     3181 cggtggcacc aaacaccacc gacgcaggtc gtcagcagaa tcgccgcacc gatatcaagg
     3241 tcatcctgaa cgtgcagtag ccatggcgca gccgtcgagt ccgaaaaaag atgtgccccg
     3301 gtcgccgctc cgttcgcgcg agccgatctg gacgctgcgc aagccgctcc cgacgagctg
     3361 gcagttttgg ctcggattgc tgttgccggt gctgctgctg attttgtggg tgtcgctgac
     3421 ttccggcgca aagcccgtcg tcggtggggc atttttgccc aagccaaccg atgtgctgcg
     3481 tgtgcttttg cgcgacctca tcatcggcaa gcaagatgtg atcgacggac agccggtggt
     3541 cgtgcgcgtg cttctcgatg cggcgattgc ttcttcgtgg cgcatcgtgc tgtcgttttt
     3601 gctcgcggcg ggggttgcgc tgccgctggg cattctgatg ggcgcgtttg aacccgtgaa
     3661 tcgcatcgtc gagccggtga tggcgcctct gcgctacatg ccgatttcgg cgttcattcc
     3721 gcttacgatc ctgtggtttg gtatcgagga aaaacagaaa atcgcgtttt tgtttttggg
     3781 cgtctttgtg tacttgcttc ccgtcgtggt cagcgcaatt cgcgccgtgc cagaggagct
     3841 ggtgcagacg gctcggacgc tgggggcggg gcggtttcag gtgattttta ccgtgcttgt
     3901 acccgccgcg gcgcccgaca tctttgattc gttccgggtg atgaacgcga tttcatggac
     3961 gtatgtgatc ctggccgagt ttgtgaacac gcgacgcggc atcggctatc agatccagct
     4021 cgccggcaat cacctgcaca tcgacgaggt gtttgccggc atcctgatca tcggcgtcat
     4081 cggtcttttg tccgacgcca tgattcgctt ctgcgccaag ctcctgtttc catggcggga
     4141 gggtggtcat gtctgaaccg caagctgcgc aaattcccgt cactgtacat agcacggcgg
     4201 tcaagctcca cgtcgaggac ttgcacgtca cctatcgcgg acgcggcggc gaagacgtcg
     4261 aggcggtgcg cggggtttcg ttcgacatcg tcgacaagcc gggcatcggc gaaatcgtgg
     4321 tgttcctggg tccgtcgggc tgcggcaaat ccaccatcct aaaagcggtc gcaggtctgc
     4381 tcccaccgac gaaagggcgg gtgctgattg ccgatcacga agtcgatgga ccagggcgcg
     4441 atcgcggcat ggtgtttcag cagtacacgt cgttttcgtg gctgacggtc gtcgaaaatg
     4501 tcgaatatgg gctgcgactg caaggcgttc catcgcaaga gcgacgcagc cgggcgatgg
     4561 agatcctcaa acacgtcggg ctggaccagt tcgcgagcaa gtatcccaaa gagctgtcgg
     4621 gcggcatgaa acagcgcgtc gcgattgcgc ggacgctgat caacaagccg aaacttttgc
     4681 tgatggacga accgtttggg gcgctcgatc cgcagacgcg ctgggagatg cagggaatgc
     4741 tcatcgacat ctcgcaccgc gaagacaaca ccatcttgtt tgtgacgcac gacgtcgccg
     4801 aggcggtgta cctggccgat acggtctatg tgctgtcgaa gcggcccgcg gagattctgc
     4861 accgcatcga cgtaccgacg ttttcggacc gcacccacaa ggtcaaagcg agccccgagt
     4921 tccgtcagtg tgaggagcag cttttgtcgc tgctatatgc caagtgagcg agcgagacac
     4981 ttcagcacgc ggcaaaccga gcgagccgcc gtatgccaag tacgcattcg cgaatcccta
     5041 caacctgtcg ctgctcgtcg gcgctgggag tgcggcgcta ctgaccgggc actggtgggt
     5101 tggcttgtgt gccgtcgctg cggaagcggt ctggatgctg tttgcgcccg actcgcggct
     5161 tctgcaaaaa aagtggttcg atccgatttg ggaaggcgaa caacaagaag cccgcaaggc
     5221 ccgccaagcg gccaagttca acgcgcttcc cgacgcggaa aaagagcgtg cgctgcgcct
     5281 gcgcgatcag caaaagcgaa tcgaaaagat ggcgcaggac aatgcgagct tttcggtcga
     5341 tcttttgtcg tcggacattg ccaagctcga agacttggtc gacgactttc tcgatatggc
     5401 gcaggtggcg tcacgctacg aggcatacct aaaagacatg aacctcgtcg agattgaaaa
     5461 agacatcacc cgttatagca atcaggtcga caagcttccc gtcggtgatg aacgtcgcaa
     5521 agtggcgcag aaaaacctgg aagtgctgct gtcgcgcaaa gaccgctatc tggaactgcg
     5581 acggaatctt cagacggcac gcgggcagat ggatctgatg gaaaacacct ttcgactgct
     5641 ctccgacgaa atcgttggaa tgcagaaccc aagtgagctg ggcgcccgtc tcgacgagct
     5701 gcgcgaaggc gtggcggcgg tgcgggaaac ctcgcgcgag accgagcgct acatgcaggg
     5761 gacgacacga tgactacttt gagcacttcc aaaacgaccc ggctgccggt gtgggccgac
     5821 gatattcgcc gtcgctatct gcgcggagaa tcgacgcagt ttgtgctgtt tggcaacgtc
     5881 cacgatctga ttcttgacga tcccgacgga cagatcgaca agcgcagcgc cgacgagcga
     5941 cttttgccgc tgcctgaatt tctgagcaag gtgctgctcg aaaagaagtc gatggtcatt
     6001 ttgtacaacg tgtcgacggg cgtgcgcttt gtgcgcggca agctgccggc gggttttgaa
     6061 gacctggcgc tgcaaaaaga gccgtcgaag attctgccgt tgctcgagcg cctgctgatc
     6121 acgcaaaacg gcgtcgcggt ggtgatcgaa tacgccgaaa ccgtcgcacc cgcgggtgaa
     6181 acgagctttt cgaccatcga agatcgatcg tcggtggtga cactgcagcg ctggtcgatg
     6241 ctgccggcgc tcgacaaaaa cgacagcttg gtgttcatgc tcgtcgaaaa tctgtcggaa
     6301 cttcatccca agctggttgc caatccgcgc gtggcgacgg tgcagattcc gattcccgaa
     6361 gccgaagcca ggcaagcgct gctgcggcac atccgtcccg acatgccgca agacgaactg
     6421 gagatgatga ccgaggtgac ggcgggtctc aagtgcatcc agatccaggg cattttggcg
     6481 ccgcacgatg tcccgtccga cgacgaggcc gagcgcacca agtacattgc cgagctgttg
     6541 ggtggcggcg ccgcagcgat ggagcgggcc cagaagctgg cgcagatcac caaaggcatg
     6601 ccgcgcagtg aaattcgccg gctgatcgcg cccgacggac ctgtgacgac gcacgccgat
     6661 accgacgcac gcgccgaagt gctgcgtctg atcgcggcgc gcaagcgcga aatcatcgaa
     6721 cgtgagtgct ttagcctgat cgagttcgtc gagcccaagc acggcttcga ggtcgttggt
     6781 ggcatggacg aggtcaaaaa agacctgtcg ctgattgcca agaacattcg cgaaggccgt
     6841 cgcagccgcg tcccgatggg cttgctgttc gttgggccga tgggtacggg caaaaccttc
     6901 gtcgctgaag cgttcgccaa agagtcgggt ctgacggcga tcaagttcaa gaacttccgc
     6961 agcaagtggg tcggcgccac cgaggggaac ctcgagcgca tcctgcaagt cgtccaagcc
     7021 atcggtcagg tgatggtcat catcgacgag gccgaccgcg cgtttggcaa ccaaggcgaa
     7081 gacgacggag gcacgtcgag ccgggtcatc gctcgcatca aagagttcat gagcgacacc
     7141 gccaaccgcg ggcgcgtgct gttcgtgctg atgaccaacc ggcccgacaa gatcgacatc
     7201 gacctaaagc gcgccggacg gctcgatcgc aaaattccct ttttctatgc gcagaccgtc
     7261 gccgaggtcg agccgatcct caaagcgcag ctgcgcaaaa acaaagtcca aagcgagctt
     7321 gccttcccca tcgccgggga tgcactggcg cgtatcgtcg ggatgtcgaa tgccgacatc
     7381 gaagcggtgg tgctgctcgc gagcgactat gccagccagc gcgccgagac gctcgggacg
     7441 ggcgaagtgc cggtcagcat cgacgatcta cagcgggcgg cgcgtgacta tctgccgccg
     7501 cgagacctgg agatgctgga gtttatggag ctcttggcgg tgttcgaggc gtccaatcgc
     7561 cgaatgctgc cgccaaagta tgccgatctg ccgatcgaag cgctgcaaga gcggctacag
     7621 cggctcaaag tggtggctgg tcaccgtcgg taatcgaacc gtctatacgc agttttgtgg
     7681 acctagctag gtcgaaacaa tcgtcagttc aaggagacgt gcgatgaact tctttcccga
     7741 aattcagctc acccaggcgc aggccgaagc gattgcgcgc ggtctgtttc acattgccca
     7801 ttccgacggt ctgcacgagc gggaagccgc gctggtggcg tcgttttggg ccgaggccgg
     7861 tggcagtcac aatgcactgt cggatctggc gcgtcgcggg ccgctgtcga ccgaagagct
     7921 caccgcggtg ctcgataccg aagacctgcg caagctgttt ctgaagacgg cgcttctgct
     7981 cgcgtttgcc gatggtcagg tcagcggcgc agagagtgaa ctcgtgcgca aatacgccgc
     8041 cgatctgggc cttggcgaat cgctcggcac gctcgagacc caggtgaagg aatatctgct
     8101 tttgcagctt tcgcacattc acaacacgca ggcgctcgcg cagatcgcga aaaaactggc
     8161 gatctagcgc gcggcgctac ggaaccgacg ggcaaaaggg cgctacggag cccacttgct
     8221 gcaaatcacg cagttgtctt ttttgctgcc gaagccgcag tcgccacaaa caaaggcgcg
     8281 ggctttggac gatccgaccc acttgccgca ctttacgcag ttgtcttttt tgctgccaaa
     8341 gccgtgatcg ccgcacagtc gggccggaat ccgcgtcgag cccatccatt tgccgcactt
     8401 ggcgcagttg tcttttttgc tgccaaaact acagtcgccg cacagcgacg cgggatagtg
     8461 ctctgcgagt gcgtttttgg cggtcaaaag cacggcagcg gcgagcggca actcaatgag
     8521 aaagcgacgg cggctcttgg tcagcatcgg ggaaactcca aaatcggtgg gcgtggaggg
     8581 gtcaaagttc gcttccatcc tacttcgttt ctgcacacag actgccggtt tggttcgtga
     8641 agcttgcgtg ttctgcctcg cctgtgccta agatcgcgcc gtcatggcgt acttctgcca
     8701 ctcctgcggg aacgagcaaa tcttcgaagt caaggtcggt atcaaagtcg gtcgtcgcga
     8761 cacctgtccg cactgcggca ccgacctcca ctgctgcaaa aactgtcagc actacgaccc
     8821 cggacttcac aaccagtgtc gagagcccga agcggcgttc attcgtgatc gcaccgaggc
     8881 caacttctgc ccgcactttg ggatgcgcga ctccgaaggc tggaaggaag accgctccgc
     8941 cgaggatgcg aagaaaaaac tcgcggcgct gttcaagaat ctcaaatgag cattcgactt
     9001 gccgaagagg acgcgccgtt cgatccagaa tcgcgccggc tgtgcggtga tcccgcttgc
     9061 atcggggtca tcggcgacga cggacactgc aaagagtgcg gtcgcatcgg cgaaggcgag
     9121 gagccgcacg cacgtcattt ttccgaggac gaaggccaaa cgtctgcgga cgatgtggcg
     9181 gcagcgccca ccgaaaccga gtccgccgcg ccggcccaaa gcgaaccagc gagcgacgcc
     9241 gggtttgaag accggcggct gtgcggcgat cccggctgca tcggtgtgct gtcagctgat
     9301 ggtcgatgca gcgagtgtgg ccgggtgttt gcctaagcca tcggttaccg accgggtggc
     9361 cgaacgcgca gcaagctgat acggtttttt atgaaagcga agctgaagat tgcttcgcaa
     9421 tgcaaccgca gatgagtcgg atgcttggcc atatcgcagt gctgtcgctt gccgtgcaga
     9481 tcgggtgtct tatcccgatt ccgctcgaac aacaagtgac caccgacggg ggccgcagcc
     9541 tgcaagtcaa agcggcgatt cctccttttg gcacccaacg ggcgacgtcg attctcgaaa
     9601 aatatgctta tcaagtcgac gtcgtttccg acagtgccga tgttgccgcc cgtctgtatc
     9661 tgcaaatcaa cggcacctgc tgcgacctaa acatcgacaa cccgaacgtc acccgctggt
     9721 cgcagcaagc gcaggcgcag gcggtcgact cttcgacggg aagctactcg atggtgtttt
     9781 cgcagccggt gctgccgtgc acgatggtgc cggcgggcgc gatggtttat gtggtgccgg
     9841 tgattgcgtc gggcggattt gccgatttcc agatgggcgt tcgccccgat ggccttggcg
     9901 aggtagaccg cagccactac tggacggtaa tttgtcctta agacggttgt cttcttttgg
     9961 tccgtaagga tgtcttatgc ctaaacctac ttcttatctt ctccggctcg atggtgggcc
    10021 caagcgacgc gacagggatt tgtgggcgcc ttttgtgctc gcacttgtgg cttcgcttgc
    10081 ggcatgtggc gaagaagctg cgtcggttcc gatcgcatgc agcggtgatc tgacctgtcc
    10141 cccggcgcgc aactcggcgg cttgggcggt gcagctttgg ccgacggaca gcggcgacaa
    10201 gatcggggcc gatcagcact ttttgctgac gccacaagaa ggccagcagc tcaatttcga
    10261 tgggaccgga tcggcgacgt tgcgttttcg ggcggcgtca ctgattggcg gtctggtgct
    10321 gacggcggac aaccgcgcgc tgattcatgc gcgggtgcag gcgacgctgc cgagtgcgat
    10381 tcctggtcaa aatgactatc gcttcgatac gcagacggca gagcaggtgg cggggacgta
    10441 ttctttgcgt gtgccgacgc cagccaagcc gatggatcag ctgtatcggc tgtgggtcgg
    10501 gttcgatgat gcgatgcagg ccgcgatgta tccgccgatg tggatcgagc atccggtgtc
    10561 gagcgacgtc gagctgccga ttcagctccg gccagcgaat cggcttgcgg tggtgtcggg
    10621 tcgcatcgtc aatccgctcg gcgaaggcgt cggcggcatg acggtgcagg tcttcgatga
    10681 aaacaaccag atcatttcgt cgtcggcggt gagcctaacg ggtgtgggca catcagcggg
    10741 cacgtatcgc acggtgctag acccatcgct ggcgagcaag tcgacggtga acctgcgggt
    10801 ggtggtgcgg cccggaacga cgcagcctgg cttgccgtcg ctggaagtgc tgattccacc
    10861 gccgacagca gggaccgaac ggacgcagaa cttcgtgctg ccgtcctatc gaacaccggt
    10921 gccgtttatg ttgccggtgc gggggctcgg tccaagcggc gcaacccaag aagtcccatc
    10981 ggcgcgcgtg gtggcacagg ttcatctcga agacgccacg acgctttcgg gtgtgcgcgt
    11041 gatttacacc gcgcaggccg acaccgacgc gcagggcatt gcgaaactgc agcttgtgcc
    11101 cgcgccaagt ggtggcagca acctgacgta tcgcgtgtcg attgtgagcc cgtcgcggct
    11161 gccgtatgcc tcggtgtcgc gcgacatgca ggtgggtccg acggatgggc tgctttcagc
    11221 gctgacgctg ccgacgcgat cgcggctgat tggccggctt ttgtcggcga gcggtgagcc
    11281 ggtgtcgggc gggcaggtcg tggcgcagcc gattgcaaag ccggtggaag tccgcagtcc
    11341 gctggatgtt gtgccggatc tgacgctgcc gcagacgacc acggacagcg acggacggtt
    11401 tgtgctacgg ctcgatccgg gggactacga tctggacatg attccggtgc tggggaccgg
    11461 accgcggacg tcgcttgata accagcggat tcgcgacgtt gacgtcgatt tgggcgaagt
    11521 tcggctgccg cgactggcac tggccaaagt cctggtcgtc ggaccctcgc ttgcgcccgc
    11581 gccccaggtc aaagtccggg tgctggagct gcccgatacg tcgccgcgct acggcttggc
    11641 gtgtacgctc gacctgccgt gctcgaaggt ggcgaaagtt cgcgccgaag cgtttaccga
    11701 tagcaaaggt cgcgtccagt tcctgcttcc cgatagctta ccgtcggctt cgctgcttcc
    11761 gcacagcctt ccctgatcca cttttgctga cgttggggat cagcttggca agcagcttcg
    11821 gcccggcttt cgcgtccctt gcatcgcccc gcttggcggc gagcagcgct tgcggcaaaa
    11881 cggcttgtaa caccgcatgc tgttcatcga gatagcgctg cgccaccgtc tgaacctgca
    11941 ctgccgtcaa gctgctgatg tgttcttctt cgctttcttg actcggtaga tccagcgcgg
    12001 atcgacgggc cagcgagagc gccacatcgc tgcggtgctg ccgcgctttc cacttctgcg
    12061 ccaagagctg tcgtttcgcc tgctgaagtt catcgtcgct gagcggatgg tcgagaagcc
    12121 gccgcagctc gtcgcgcaga ctggcttcgg cagaatcgag gctcgtcggc gtcgtgacgg
    12181 cagaaatcag cagcgcaccg ccctgcagcc cgccttccag cttgccatcg agcgagagca
    12241 aaagtccgcg gcgttcgacc agctcgcgat ggagccgacc cgctttgcca acgagaattt
    12301 ccgacaagac cgccagagcc agcgcatctt ggcctttcag tccgggcgtc cgaaagccaa
    12361 gccccaggat cgcctgctcg tgcgggtgat aggacaaata ctggcggggt gtttcgatgg
    12421 tggaaagcgg cgatggcggc ggactggcgg tcttgccgct gttttgcagc cgcgactcga
    12481 tgagcgaaaa cacggcgtag ggttcgatgt ctccgacgat ggccagcgtc atttgcgacg
    12541 gaccatacga ccgtcgaaac agctcaccca gccgcttgcg cgaaagcccg gacagcgact
    12601 gcaccgacgg ttccagcttg tacggatggc tgccatacag cttcgctttg agcttcgcga
    12661 gcgccactgc gcggctttcg tctttttgcc gcagctccgc gacaagctgt cgacgccgca
    12721 gctccagttc cgcatcgctt ggttctggcg ccgaaagtcc gtcgagaaac agcggcagcg
    12781 cttcctcttc ggcttgactt ggccactcac cgaccagcga aaacgcatcg cgttcgactt
    12841 gggttgcgac ggtgcctcgt agctgcgcca gcgtgtcggc ccactgacca ccgcgcgccc
    12901 gcggcgtttc tttccacagc cgactgatca gctcatggcc ccccgccgtc tgttcgtctt
    12961 cgagcgaaag accaccaggc caagtcgccg ccgccgcgac gatcggaacg ctgtgatcgg
    13021 gaagcaaaag cacaagcggc ccacccggca cttgatagcg ccagaccccg ccgtctttgc
    13081 gtgctgccgc aagcggcttt tggaccgtcg gtggtggcgt ctttagctgc gcgagcaggc
    13141 ggttgcggct gcgctcgagc tgcgccgcgt cgtctttggg tgtgcgcgac ctgggccaaa
    13201 gccacagacc gatgagcgaa tcgcgctgca ggtggcgtag cagaatctga cgcagcgacg
    13261 catcgcttag ctctcgcacg gcttggttgt aacgatcttc cgacagcccg agcgatgtaa
    13321 agtaacccgc ccgtcgcgcc cgaccacttg gcgtatcgag cagccgcgcg ttttcggcca
    13381 gaagctgctg tttggcgcgg gccagctctc ccggttccag gtcccgctgc gaaggccgca
    13441 acagctcgtg gagcagtttg gcgctcattt cctcgacttg cgacggcggc accatcatgt
    13501 gggcaaagaa aagccccgac tgccgaccgg aaaacgacag cgccgcgggt tcggacttgg
    13561 gccggctgcc gaggctgcga aacccgaggc tgctgcgtac aagctgggca aacacatcaa
    13621 atgccgcgac ttcttcttcg gcacaagcga caccggggag agcgaaagca acgacggctt
    13681 gacccgatgg atcatcgtgg cttgcaaccc aggcctgcgg tgaatgcgga cgggtcagtg
    13741 tctgcgtggt cggctgggtc gctgcggtct gcggggccgc cagacccgaa agcagcgatg
    13801 tcaaaggatg ggtcagcgtg tcggcgagcg ctccacttac caaaagcgat agctgcgccg
    13861 gctgataggt ctgccgataa tacgcgagca catcgtcgct gcgcagcgca gacaaacgca
    13921 gccgatctcc gagcagcgga cgggcgtagg gatgacccgg aaacgacagc tcgcggagtt
    13981 tttcgacggc gacgctgcgc agcataagct gggcgcgctt gtgttcgtct tccacctgcg
    14041 caacctgtcg cgcaaccatc gcatcgtcga cagtcggcgt gggcataagc actgctgcca
    14101 aaaaatcgag cgcgagtgcc tgctggcctt tgggcaaaag cagctgaaac accgtgtgat
    14161 cgagcgtcgt aaacgacgag acatccccac ctgcggcgcg aatgttgtcg ctcgcggact
    14221 tggccagctc gtgcaaaagc agccgttcgg tcagatgcga aagtccgaga ccgctcgccg
    14281 gttcactcgc gacaccacca gaaacccaca actgcacggc cacggtaccg accaagcgat
    14341 cgggcagaag ctggattcgc acaccatttg gcaagctctt ccatagcggc gcgcttgggg
    14401 tcacttcagg tggtggggcg gacagaaggt tttgcgtgtt gggcggtggt tcgaccgttt
    14461 caacgtgggg ttgcgaaggc aagagcggct ttggatcgac cagcgctgcc acggaaatcg
    14521 gctttggcac gcacgcaaaa agcggcacca gcaaaagccc tgtcgtcgcc agacggccta
    14581 ctatacgcat cgcgcagtgt cgtaccgcaa agcgcgcagc gacgccaagc gagaaagctc
    14641 accaaggacc gtgcaggcaa actctggccg cttttttgcg caaagctctg gcatcgtagt
    14701 agctgtccca tgaccatgaa cacgctcact tcgattccgt cgtcgtcgtc tcacaccatg
    14761 ccgctgactt tgctttcgtc gccactcgac gctgcgatgt cccacctccg ctcgcttcag
    14821 aaagccgacg gaggctttga aggcgagatg gtctggaaca cgatgatcct ttcgcagtac
    14881 atcctgacgc agcgcgccat cggcaagccg atcgacaaac agaccgccga caagatggtt
    14941 ttgcactacc gcacaacccg ctgtaaggac ggcgcttggg ggatgcacgg ggaatcggac
    15001 gggtatgtct atttcaccgc gatggcgtat gtggcgctgc gggtgctcgg cgtgtcggct
    15061 tccgatccgc tgtgtgccga tgcgcgggcg tggcttgcgc ggcagcccga aggcgtgctg
    15121 gggattccgc actggggcaa gttttggctg gcgatgtgcg gtctgtatgg ctgggatggc
    15181 gtaaatccat tcccacccga gctgtttgtg acgcccaagt ggctgcccat ccatccgttc
    15241 cgtttttaca accacacgcg ctacatctac ctgggcatcg cgtatctgta tgggaagcgc
    15301 tttcagattt cgcttggcga tcttctcccg acgctgcgca gcgaactgta cgatcaagcg
    15361 ggccggcgtc cgtatggtga aatcgacttc cacagctatc gccacgtcgt cgccaaaacc
    15421 gatctgtatg tcgcgccgag cctgccgctc aaagccgcgt atgaagcgct gcacgcatac
    15481 gaaaaaaggc cgattgccgc gctgcgacag tacgcgctgg gattctgtct ggatcgaatt
    15541 ttgtacgaga tgcgcgcgag caaatatcag tgcatctcgc cggtaaacgg actcctaaac
    15601 tgcctggcaa tctattccca caacccgcag catcccgagc ttgcgagctg tcttttgggc
    15661 ctggaagcat ggaaatggga agacgaggca gaaggaatcc gctactgcgg cgcgcgcagc
    15721 aacacctggg acaccgcgtt tgcgattctg tctttgaccg aagcggatcg ggcccagcag
    15781 cttgcgccgc acaatgccga aacgctgcga cgggcgtatg cgcatttgtg cagcttgcag
    15841 atgcagaccg aactggatgg ctgggaagcc gaacagcgcg agccaatccg cggcggttgg
    15901 tgtttttccg acggagaaca tcgctggccg gtgagcgact gcacggctga agcggtctgc
    15961 gcgcttctgt ctttgcacta cgaagggagt ccggttccgg aatccgatcg tttgcctgta
    16021 cgccgttttg tcgaggctgc acacttcatt ttgcggcgcc agaacgaaga cggcggcttt
    16081 ggttcctatg aagcgcggcg cggcggatcg ctgctcgaaa aggtcaatcc gtcggagatg
    16141 tatggcgcgt gcatgaccga gcgttcctat ctggaatgca cgtcgtcgtg tctgcgcggt
    16201 cttttggcgc tgcgcaatcg gctgggcgcc aagctcggtg gctcgctttt gctagagatc
    16261 aacaccgcga ttggggcagc cgtcatgctg ctgcgcaaaa gccagcgtcc cgacggctcg
    16321 ttccctggat tttggggcat caacttcacc tacggcatct tccacgttgt cgaagcgctg
    16381 cacgaagccg ggattccgtc caccgatccg gcgatccagc gcgcggcggt gtggctgctt
    16441 ggcaaacaga gacccgatgg cggttttggc gaacactatt cgagctgcct gaccggcgag
    16501 tacgtcgccc attccgagtc gcaagtggta aacacaagct gggcactttt gacgctgctt
    16561 ttggcgctgc cgccatcgca cgaagcgacc cagcgcgggc tcgattttct gtgcaagatg
    16621 cagggcaaag acggtggctt cccgcagcaa gcgcaagcgg gggtgttttt cggcaccgcg
    16681 atgctcgact accggctcta caagtcgtac ttcccgctgt gggcgctttc tcgcgcggcg
    16741 cggctgggac aggagctacg tcatgcccgg acctcccatc cgctcactcg ctgacgatca
    16801 cggcatccgc gcgacgctgt tttcggtcgg gcggacgcgg cgcaaacgga cgcggatttt
    16861 tctgccgatc tttttgggtc tgttttcggc agcgcttctg tttggtctgc tggtgctgcg
    16921 ggcgtggctt ctgccgcgct ataacgacaa gtttggcgac gcgccgcttc tgggtcatct
    16981 ggaagtcgat cgcaagctgc cagcgcagac ccaccgcatc ggaccgtttc agctttcgtt
    17041 tacgcccgat tcaggcgggg tgctctacgt cgtgcatgaa aaggaacctg agcggcggat
    17101 ctgggaaacg ctgcccggca tgtcgtttgt cgctgcggct tacggacatg aaaaagtcga
    17161 tgaatcgcgc ggttcgtttt tcttttccga caaagcgacc ggaagcactt gtgcgacgca
    17221 gcggctcgat cagattgcgg ggtcaaatgg catcgtcaag ctgtcgggaa cgctgcgctg
    17281 tagcggtgct ggatcggccc cttatgtgct cgtgttttcg caggtggcgc aaaatcagct
    17341 cgggtttgag ctgcggattg aagacgacga tctgaaccgc gcttttttga cctatgcatc
    17401 gccgacaacc gagcacgtct ttggttttgg cgagcagttt acgtatttcg atctcaaagg
    17461 ccggcgggtg ccgatttggg tgcaggaaca ggggctcgga cgcgggcttc agccgatcac
    17521 caccgccgtc gatctggtcg cgcgatccgg gggaagctgg cacacgtctt acgccagcat
    17581 tccgcactac atcaccagca cggcgcggag cctttttacg gaaaactacg aataccaaat
    17641 cttcgatctg cgcacgccgg accgcatcca agtccgggtc tacagccgca cgatgaaggg
    17701 acggattctg tatgggaaaa cgccgccgga tctgattcgc gaatacaccg aatacgccgg
    17761 cagaatgcgt ccgcttccgg actggattca tcagggcgcg atcgtcggca ttcagggcgg
    17821 aaccgcgcgg gtgcaggcgc ttttcgacaa gctgcaagca gaaggcgcac cgctgtccgc
    17881 ggtgtggctg caagactggg tgggtcagcg caagaccagc tttgggcagc agctctattg
    17941 gaactggcag ctcgataggt cgcgctatcc cgattgggaa gcgatgcggc gcaagctggc
    18001 ggcgcaaaac gttcgcgtct tgacctatgt gaatccgttt ttcgtcgaca ttgccaagct
    18061 gggccggccc ggacgcaacc tgtttgccga agcgcacgat cgcggctatc tggtgcgcga
    18121 accaaacggc cagccgtaca tgattcccaa caccgacttt tcagcggggc tgcttgatct
    18181 gacccatccg gaagcgcgca agtggtggaa agacatcatc cgcagcgagg tcttggcgac
    18241 cggcgcatcc ggctggatgg cggactttgg cgaagcgctg ccgtatgacg cacttttgca
    18301 ttccgatgtc gcggcgcggt cgtttcacaa ccaatatccc gaggaatggg cgcagctaaa
    18361 ccgtgaagcg attcgggaag ccggtcgcga agacgatgtg gtgttcttta cccgatcggg
    18421 atatcgcaaa agtccgggca gctcgacgct gttttggctg ggtgatcagc ttgtgacctg
    18481 ggacgtccac gatggtttgc gcagtgcggt ggtcgggatg ctttcgggcg gcgtgtcggg
    18541 tttttcgctc aatcacagcg acgcgggcgg ttacaccacg attgtgaatt cgatcctgaa
    18601 agtgaagcgc agccgcgagc ttttgtggcg ctggctggag ctttcggcgc tcagtccggt
    18661 gatgcgcacg cacgaaggca atcggccaga tgacaacttc cagatcgatg gcgatgcggc
    18721 gacactgcgt cactttgccc gctgcgccaa gctgtacaaa gcgtgggggg actatcgcaa
    18781 acagctcgtg gcgcaggctg cggcgacggg aatgccggtg gcgcggcatc tggcgctgca
    18841 ctatccagat gatccgaatg tctacgcgct gtcgtttgag gaatacctgc tcggcgaaga
    18901 cctgctggtc gcgccaatca gtgaacctgg cgcaaaccag accaaagtgt atgtacccga
    18961 aggcacctgg gtgcatgtct ttaccgggca gagcgtcacc gcgggcaaga gcggtctgcg
    19021 tgtcgatgtc gaagcgccgt ttggcaagcc ggtgatgttc tatcgccaag gcgccgcaat
    19081 ggtcgcgccg ctgctcaaag cgatgaagga acagggtctt ttgtagcacc catcgcaaag
    19141 cgcgcttgtc ggtgaatcat ccgaccaaaa ggcgcggttg caaaaagctc acatatggta
    19201 tgtatgtcgc acttcttact ccctgaggag ggtgcgatga aatgtaagct tgccgctcgc
    19261 tcgccggacg ctgataaatc cagcgtcacc tctcggctca ccgggacact gcgtcactgc
    19321 ctggcttggg tggcgacgct ggctgtcctt ggggccgctt cttctgcctc tgctgcgggt
    19381 ccgaaaggct gcgtcgtgta cgccagcacg gactcatcag taacgggcga catcaagtcg
    19441 aagctcgaag gaaccaagct cttcgactcg gtcacgatga ttgacggcgg atacaccgcg
    19501 ccggcagatg ccacactcgc gaagtgtgat tcggtgctgg tgttcggcga ctacaacaag
    19561 ggcggattca acgacccgac gggcgttggt gacgcgctgg ctcgctacat cgacaaaggc
    19621 ggtggcgtcg tcgtcgcgct gccgttctac ggtggctatt actacaacgc tccgacggga
    19681 acgaactggg ccaagtacca gctaatcgac aactcgtcgt cgatgtccta tgtcagcagc
    19741 aagtcgctcg gcagcaagga cgcgcacccg gtgctgagcg gcatcagcaa agtcgacaca
    19801 aacggcagca cctgccgcga gcgctcgagc gtcaccagct cgacggtcaa gtcgggcgcc
    19861 aaagtggtcg cgacctggaa cgacggcaac ggcatggtca tcgcgggttt ccccaatggc
    19921 cgtccgcgca tcgacgtcaa catctacccg gtgtcgagcg acattggcta ctacggctgc
    19981 tacaactcgg cgtcggatgc ggccaagctg attgcgcagt cgatgatcta cgtggcgagc
    20041 ccgcttcgtg caaacccgtc gcccgccgac ttcggtgagg tagcggtgct cggcagcgcc
    20101 gatctcaacg tcgactttga aaaccagggc tcagcgccga tcacgattgc cagcggcgac
    20161 atcaccccga cgggcgtgtt caccgtggac accgtgagct tcccaaagac gctcaacccc
    20221 ggcgacaagc tcagcctgaa gttttccgcc aagccgaacg ctgccggcca gctcagcgcg
    20281 atttatacgc tgactccgtc gacggcgggt gtggcgccgg gcgtcgtaca actgacggtg
    20341 aagggcatcg gaccgcgcta tgacgcccag ccgagccaaa tcgactttgg cggtgttccg
    20401 gtcggcatga cggcgtcggc agtgacggtc aatgtcacca acaccggcgg cggctacttg
    20461 ggctttaaca ccgtgtcgat cgacgatacg accaactttg cgctcgaaaa cgtgccgatg
    20521 gcgacgctgc tcggctcggg cgcgggagtc agcttcgacg tcaagttcaa gccgacgctc
    20581 gaaaaggcct ataccaccaa cgttcgcgtc gcctactcga taagcggcgt gaactactcg
    20641 ggcatcgtga cggtcaaggc atcgtatggc aagccgaaga ttgtggcgcc ggcctcgctg
    20701 gtgctgtcgc cggttcgcgt gggtgccacg ggtccagacc agatcatcac gctccagaac
    20761 accggccaag ccgacctgag catcaccaac atccagttca ccagcggtga catgagtgac
    20821 tttgcggtgg tgacggcggc ttcggccatg tcgccgttta agatcaacac cgcgtcgtcg
    20881 ggcgacatca aggttcagtg caagccgggc gtccagggca ttcgtcaggc aaaccttgaa
    20941 ttgaccagca acgatccgtc tctggcgatg ggcaagacca acatcgcggt gtcgtgccgt
    21001 ggcaccgtgg ccaacttcga gctgagcgtc gacaagatcg acttcatggg caaccagcag
    21061 gctggtcagt gctcaggcgc gcagtatgtc actgtgaaaa actcgggcac cgacgcgctg
    21121 aagatcctgt cgctggcgtt tacgggcacc aacgcttcgt cgttcaagca ctcggcaccg
    21181 gcgacgcgca cggttccagg cagcggcggc acgtttgtga ttccggtgca gttctgcccg
    21241 gtcgacatcg gcgcgatgag cgcagcgctg gaaattgcga ccgactttaa gccgggtcac
    21301 gttgccaagg ttccgctgac gggtacgtcg acgggtccaa aagtggtggt gacgccgggc
    21361 aatatcgact ttggtacggt ctacatcaag accacgtcta cgtcgaagca gatcaagatc
    21421 accaacgacg gcgaccagga tctggtattc ggcaagagcg tactgacgcc ggcgatgggc
    21481 gcgtttaagg tcgcgggtct gccgacggaa ggcaagatcc tcaagaaagg cgatccggcg
    21541 gtggttctcg acgtgcaagc gggtccggtg acggcgatgc agcagagcgg cgagatcagc
    21601 atcatcgtca acgaccaaat caagatgaac aacatccgga ttccgctgtc ggtgacgggc
    21661 gtgcaggcgg acctcaaggc cgatccgatg atgatcacct tcccgctgac gattgttggg
    21721 ctgcgttcgc aggagcagac ggtgaccgtg accaacatcg gcgcggctcc cctgacgggt
    21781 cttgacctca gcgtgagcgg cacggcgagc agcgactacc tggtcagcgg cgatcgcacc
    21841 gtgacgattc cgccgtctgg caaggccgag ttcaaagtca ccttcaagcc gacgggcagc
    21901 ggtccgcgca gcgccatttt ggtggtcaat gctgcgggtc tgatgacgcc gacgcaggtc
    21961 aagctggaag gcaccggcaa gctgctcacc gtgagctgct cgcctgacga caagaacctg
    22021 ggtatggttc gcatcggcga atcggtcagc ctcaaggtga tctgccgcaa ctccgactcg
    22081 agcgacatcg acttcgtgac cggcttctcg gacaacccgg atgactggtc ggtcgatccc
    22141 gcgatggaca agctgccggc tggtacgccg acggaagaag gcctggcgac gctgaacatc
    22201 accttcaagc cgacggtgac gggtccgcgg acgacgacgc tgacgctcaa gaccaaagaa
    22261 ggtctggtga tcggctcgat tacgctcgac ggtacgggtc tgccgatgcc gaaggaaaag
    22321 atgatggaag atcaaggcgg ttgccaggct ggtgggcgtc catcgtccgc agcgggcacg
    22381 cttttctttg cgctgctttc cgttggtacg ctgatcctgc gccgtcgtcg cgccgcgctg
    22441 taatcgccaa aagccgacct acgaatatag taggtcggct ttggttccca ccttgtcgct
    22501 actccgcttc gcgccgctac tccgcttttt tcagcaaacc ctctcggctt ccgcacgcga
    22561 caatttcgtc aagctgctgc tcgacgagcg gtgcaaacgt gcagccgctg tcgatgtgca
    22621 gtccgggcag tgcgctgggc cgaagtgatc gtcgcaatcc aagctgcgca ggcatctgtc
    22681 cgggtcgggc tccactcaca cggtagcttg gggacagcgg tccgaatcga gtggtctgga
    22741 gccgcagcca cagatactgg tgcgcggttt ccaaaagacc cgcgtcggcc agcgcttgcg
    22801 ttaccgtctg cggccacagc ggggcttcgt tgtcgcttgt ttggtcgtgg acgaaaagtc
    22861 cgacgatcgg ggccggctcc agcgccaaca agcgaccgcc cggcgcaagc acccgcacaa
    22921 gctctggcag aagacgcgac agcggtcgaa tgtgatgaat caccaaattt gcgacgacaa
    22981 aatcaaagct tccgtcggaa aatggcagat ccatcgcatc ggcgatgaca cagtgcagtg
    23041 gcccatctgc cggctgactc gctgccgccg cccacagctg cttggggact gcatcgaccg
    23101 cccacacttc gctcgcgccc gcaacgagca gcgcttggct tagcatgcat tcgcctgcgc
    23161 cgatttccag cacacgcttg ccggcgagcc gcgttcgatc gagcagaccg agcgcctgca
    23221 ccacgccgag accgacgaag tgaccttggc gctgccacgc cttttgacgc gcaagctggg
    23281 cgccaagctc ggcgggatcg acgcggtaat agtcaaactc agcatcggcc cgccacggcg
    23341 gacgcggcac catcagtcgg cggacgttct gtagccacgc cgaggccagc gcaggcagcc
    23401 cgtgcccttt cacagatgga ctttctgtcg cgcttcctcg agcattttcg ggtgctcggc
    23461 gggatcggcc cagcgcgctt gtccctcggg cggcagacgg tcgatcggat caaaataaaa
    23521 gaacagctgg ccgggcttta ccgccggggc cgaatagacc gaaacccccg tcgtgcggtc
    23581 gtagtgttca aatgagtgcg catctcgctt gccgccacca ccgggcgcgt cgacgacgaa
    23641 cgttggcgtg ttaaagcctg ccgtcgatcc gcggagctgt ttttcgatgt cgagcgcggt
    23701 ctgcagcgtc gtccgcatgt cctcgacgcc tttcaccatg tcgtgaatgt agacgtaata
    23761 cgggtggacg ctgatgtagc cgagccgctt gagcagctgc cccatcgtcg ccaccgagtc
    23821 attgacgccg cggatcagta ccgactggtt gcgcacaaaa atgccgcgcc caaacagcac
    23881 ctggatcgca tcttgggtga tgccggtgat ctcgttgggg tgattgaagt gggtgtgcag
    23941 catcacgtct ttgccgagct tgcggccctt ttcgacgacg taagtcagcg catcgaccca
    24001 ttcgtgatcg gtcagcagtt tttgtggcat caccgccgga cccttggtgg caaaacgcat
    24061 ccgtcggatg tgatcgatgc caagcagtgc atcgccaatt tcgcgaatct gctggggccg
    24121 caggttgtag gcgtcaccgc cggaaatcac cacgtcttcc aactcgggtc gcgaggcaat
    24181 gtaagcaaaa gcttttgccc agcgctcctc actcacgcgg agcgtgaatt tttcgacttg
    24241 tttggtatcc acaccgacgg catagctgcg ggtacagaag cggcagtaca ccgggcaggt
    24301 gtccagtgcc aaaaacagcg ctttgtcgac gtagcgatgg gtcagccctg gcgtcggcgc
    24361 atcggctcgt tcgctcagcg aatccaggcc cagcttggga tggtccgcca tcagccgcga
    24421 gcgcaccgga ataaactgga tgcgcagcgg atcgcggtac ggattgctcc agtcgatgag
    24481 actcagcagg tacggggata cgcgaatgct catcggcgcc tgctcgaagc cggcggcgac
    24541 gtctttgtaa aactccggcg aaaccagcga ctgcaccgcc gcgagcagct tgtccggatt
    24601 ggtgatcgtg tgcttggcct gccacttgtg gtcgacgaag gtctgttcgt cgacttcagc
    24661 gtaggcagga atgtgcgacc agaatcggtc acggcgcagc tgacgatggg tcagcgtcgc
    24721 gggatcgacg gtcggtctca gaccatgggg gacatcggtc gacgctacta cgggcagctt
    24781 ggcagttcct gagttgtcgt tcatcgaaac accgtctgag cctgcaatcg cataagcgag
    24841 ccaggctggt ttacaagtcg tggtggtccc gtcggtgcag acagtgcagc gacgcagaca
    24901 cgggcaaaga cgtactgcgc tcggctatac gtcaggctcg tgacggatgt tggtaagaaa
    24961 ccacacgata attctcagga tagagaaggt aactgctcag tgtcaatcgg tgcgagcgcg
    25021 atcgtcccag cgtttgcact ctgcttcggc aaacttcttg aattcaccgg ccaagatggc
    25081 ggcgcgggcc cgctcaacca ggttcaagta ataggtcaag ttgtgcagcg tcgccaggcg
    25141 agaaaacagg atttctttgg cgataaacag gtggcgcaaa tacgagcggg tgtagctgcg
    25201 gcaggtctcg cagctgcaat cgggatcgag cggcgacagg tcgcgctggt atttggcgtt
    25261 gcggatgttg accttgccaa gcgacgtaaa aagctgaccg ttgcgcgcgt ttctcgtcgg
    25321 catcacgcag tcgaacatgt cgattcccga tccgatggcg tgcaccagat cgcgcggcgt
    25381 gccgacgccc atcaggtaac gcggcttgct gctcggcagc tcgcaggcga cgatatctag
    25441 cacttcgtac atcgtctgga tcggctcacc gacggcaaaa ccaccaagag cgaaaccatc
    25501 aaacggcagc gcgcagattt cggccatgtg ctgccgtcgc agatcgacgt gaataccgcc
    25561 ttgcacgatg ccgaaccgtt tttggtggcc cagcgggttt ttcccgtcag taagcatcgg
    25621 cgcaggaacc gacagacagc gcgccgccca agcggttgtc ctgcgcatcg ctgcttgcgc
    25681 ctcggcgggc ggcgacagcc ccggcggaca gtgatcaaac gccatcgcaa tgtctgaacc
    25741 cagcgtcgcc tgcacccgca gcgactcttc cggcgtcaaa agccgcagcg atccatccag
    25801 gtgcgatcga aaacggacgc cttcgtcggt gattttgaca agcccaccct gtaccgccgg
    25861 acgtgatcct gcttcttgcg tcgtggccgc cccggacgcc attgctgccc cgaggctgaa
    25921 gacctgaaat ccgcccgagt cggtcaacat gggccgtttc cacgtcgtga agcgatgcag
    25981 atcgccgtgc gccgaaatca cgtccatccc tggacgcagc cacaggtgat acgtgttgcc
    26041 gaggatgatc tgcgcaccga gctgcgtaag ttcgggcacc gtcatcgctt tgacgctgcc
    26101 gaccgtaccc acgggcataa aaatcggtgt tttgacaacc ccatgcggca acagcagccg
    26161 tccggcgcgc gctttgccat cctgtccgtc gagctgatag atcggcccgt ctgccggcgg
    26221 cgtcgcgggt agctcgtgct cggcctgact taccaagcgg tttgcatgcg atggaatcat
    26281 ggcgcgcgca gtctacatcc gaccgggcaa aagcatcgcg tcgccgtagc tataaaagcg
    26341 atagcgggcg gcgattgcct gcgcataggc tgccaaaatt cgctcgcgac caacgaacgc
    26401 cgcgaccagc atcagcagcg tcgatcgtgg caggtgaaag ttggtcagca gcgcatcgac
    26461 aacgcgaaac gcatagcgac ccgacggcgc ttgcggatag ataaaaagct gcgactgtcc
    26521 catgccgggt gtcggcacgc tttcgccttc tttcgtcgca gattcgagcg tgcgcaccac
    26581 cgttgtgcca atcgccagca cttttcgtcc ctcgcgtcgc gctctggcaa tcgcgtcgct
    26641 cgtcgattcg gggatgtggt agcgctcggc gtgcatcaca tgtttttgcg gatcgtcgtc
    26701 gcgaagtggc aaaaacgtgc ccggcccgac gtgcagtgtg acgctgtgtt tttcgattcc
    26761 gcgccgcgac agctcggcca aaagcgtgtc ggtaaaatgc agccctgccg tcggtgccgc
    26821 gaccgatccg agcactttgg catagaccgt ctgatagcgc gaatcgtctt ctttttgcgc
    26881 gatgtcgccg gtgagctggc gctgacggac gatatacggt ggcagcggca gctcacccgc
    26941 ttgggaaagg acttcatcaa gcgcaacatc cgtcgggaaa tcgacgattg cgcgaccttc
    27001 gccttcgacg cagcgcaccg tcagggttcg attggcaagc ggctcgccgt ggcgatccaa
    27061 gatttgcagc gtcgttccgt cgcgcagcgg cttgctgacc cgatacatca cccgccagct
    27121 ggtcttgtgg tcgtcgccaa cgtgcggttc caaaagcagt agctcgacgc ggcccccaag
    27181 ctgaccatcg ggacttcgtt tcttggcgta gagccgagcc agcagcacct tgctgtcgtt
    27241 tacaaccagc agcgtgccgg gcccgatttt ttgttcgatg aggtccggta gcgacgaaaa
    27301 cgtgtggtgc gaaagcgtgt cccgttcgac caaaagcagc cgcgaaaggt cgcgctgttc
    27361 tgcgggtcgg gttgcaatca gctcgtcggg aagctcgtag tcgaagtcgg cggcagaaag
    27421 ggcagtcatt acgtcggaga tttcgcgagg accgagtgac gtttgccgta gctgaagtag
    27481 atcacaagac caatcgccag ccaggcaaag aagttaagcc aagtcgcctt gtcgagaccg
    27541 gccatcattc ccagacaggt cgccatgccc agcaccggca ccaccggcac cagcggggtc
    27601 ttgaacgggc gctcgcgcgt cggatcgctc ttgcgcaaca ccagcacgcc aagacagacc
    27661 agaacgaacg caaacagggt gcccatcgac gtcaagttgc ccgcgacttc cgtggacatc
    27721 gtcgacgagc acgccgccac caccgcgccg gtcaggatcg tcgaacgaaa tggcgtctga
    27781 tatttggggt gaacttggct gatgctcgcc ggcaaaagtc cgtcgcggct catcgcgaag
    27841 aacacccgcg gctgacccat catcatcacc atcacgacgg aaccaagtcc ggccagcgct
    27901 ccgatcgaga ccagcatcac gccccacgac accccagccc gcgacagtgc cagcgtgacc
    27961 ggcgccggat ctttcagttc caggtacggc accatgccgg tcagcacagc agcgacgaga
    28021 atgtacagca ccgtgcagac gacgagcgac gcgatgatgc caatgggcaa gtcacgttgg
    28081 gcgttcttgg cttcttcgga catggtggtg acagcgtcga agccgatgta cgcaaagaag
    28141 atcgtgccgg cgccctggac gatgccccac ggaccaaacg gaacaaacgg cgaccagttg
    28201 gcggtgttga tgtgcatcgc gccgatgccg atgaacagca gcaccgcgat gatcttgata
    28261 aacacaatcg ctgacgtggc gcgggcgctt tctttgacgc cgacgaccag cagcaccgtg
    28321 ataagcccga cgagaatcac cgccgggatg ttgaaaatgc cgtgatggac gatgccggca
    28381 gcgtcggtct gggtgaccat gtagccgttg acccactcga aaggcagttg gatgtgaaag
    28441 cctttttcga gaaagcgcac caagtagccg gaccacgaaa tcgcgacggt gatggcgccg
    28501 acggcatact ccaaaatcag cgcccagccg atgatccacg cgaacagctc accgagcgtc
    28561 gcataagcgt aggtatatgc gctgccagcg acgggaatca tggctgccat ttcggcgtag
    28621 cacagacccg caaacgcaca gcccagaccc gaaagcacaa aggacagcac cacggctgga
    28681 ccggctttcg tcgctgcggc gtttcctgtg agtgcaaaaa taccggctcc gatgatggcg
    28741 ccgatgccaa tcatcgtcag gtcaaacgcc gatagcgaac gttgcaggcg actgcccgaa
    28801 ccttctgcct cggcgatgac tttgctaatg gacttggtgc gaaacagcgg agtcggcacg
    28861 ggtcctccta gttgggcaat gtcaccgacg gggtgacgac gcaaagagcc accttacatc
    28921 agaccgcctt gtgcgcggca gaaaaatcgt cgagccaccg cgtgcaaagg ctgtttgcag
    28981 aagccattgt gcggtttgcc tatgacgccg gagtgctcgg gccggggtga cggtagagca
    29041 caaacgtgaa catcccccaa agccacgctt cgccgcgctc atagctgact ttgggctcga
    29101 tttgttcgtc gacaacgtgc gcgcaactgc cgtggagccg cacgacttcc tggccgatcc
    29161 gctcgacggc gctgtgcatc aaatcatcgt agcagtgcgt ccacagctcg tcgcggccaa
    29221 ggccggtgtt gggaaccggc gggctctcgg cctcgacgac aaattcacct gcggtttcgt
    29281 cctggagcgg cggtagttca cgccgaaagt agaccagctt ggcggggctt tctgcgcgca
    29341 tggctagaag tcgtcgtcgt cgtccttgcc gcctttgatc gactggagca gggtgtaggc
    29401 ggctttgact ttcggatggc tcgggttgtt gccgaacatc tgcatgactt tgcggcagtt
    29461 gttgcgcgcc tggccggggt ttttcgccac catcgacttg gccgccgcaa gcagctgatc
    29521 cgcttgtgcg tctttggccg gatcgccgcc cgctgccggc ttggctgccg tttggccgcc
    29581 gcccgccttc gactgaagac ccgaaatttt gccacccaaa aagcccgaca gacccccacc
    29641 gagccgcttg tccgccgtct gtgcagcttg atacgcggcg atcgcgtctt tgggactgct
    29701 gccttccgat ttggcggctt tttccacctg cgtcttgacg tcttggatgt ccttgatcgt
    29761 cgctgctgct ttcggatgct tggtcgcgga aagcgccgaa atcgccccag caaagtcgcg
    29821 atttttgtac gacgacgccg cctgtttgtt ggtgtccaga tcgccgcccg ccgctgctgg
    29881 tgccgcttcg actttcttga ctggctcggc ttttttgacc ggctcggctt ttttgaccgg
    29941 ctctggcttt tcgacttttt cgaccttttc gactttttcg ggctcggctg ccgcgacctg
    30001 cgtcgtttct tccgtcttgc cgccgccgct cagacgggct ttcagctcca gcagctcgct
    30061 gctttcggga tcgatttcca gaccctggcg cacccgatcg agcgctcctg acgtgtcgct
    30121 cttggcaagc gagtttgcct ccgagaccag cgctttgatt gcttctcggc gggccagccg
    30181 cgcttgctgc tgggcgtcct cgtacagcgt cgaatctttc ggtacttttt caaactcttt
    30241 gagcgcgtcg gcgtagcttc gcttgtcgag cgcgcgtttt gccgcatcga gcgccgcggc
    30301 catcttcgct tcgctatcgc acagctcgat gtagcgcttg agtaccgtcg agtcgggagc
    30361 tttttccgct gccgcctgga acgctttttt ggcgtcgtcg taatggccgg ctgtgaaatt
    30421 cttggtgccg gtgacgtact gatccatcac cgcgtccagc gctttgccgc cacccgacga
    30481 tcgcagcgcc gacgtcagaa tcagtccaat gccaccgacg accagtgccc cggcgatcgc
    30541 tcccagatag agcgttcgtt tgcggctgtc agacagaaat gccagcggtc cacctgccgc
    30601 gccttctccg cccatcgaca tcgactgcga ctgcagcgac gcgggcgtgc cggtcatcat
    30661 cggtggtgat gcgacggctg acgttggcgg cgaaagtgcc ggcggcatac ccggctgcgg
    30721 cgcaaagccc ggctgcggat aacccgacgg cggatacccc attggcggct gcccagcgta
    30781 tgagcccatc ggctgaggca tcatcggctg ctgcatcatc ggctgaggca tcatcggctg
    30841 ctgcatcatc ggctgaggca tcatcggctg ctgcatcatc ggctgctgca ttggctgggg
    30901 aaccggttct gcctgcgcgc gcgacggcga gtgctcaaat cgcatcgtcg tgttgccgat
    30961 ctcgatctgg tcgttgtcaa ccagttggcc ttcggcctgc cgcttgccgt ttaccatcga
    31021 gccgttcggc gagcccatgt cctgcatccg atagccgttg ccggacatgt ggatttgcag
    31081 gtgtttgcgc gaaaccgcga tgtcagcgac gatcaccatc tgatcggcac cacgacctat
    31141 cgtggtcgac gtgtgcttga gctcgaacgt cttgccttgg tcgttgccgc ttatcacccg
    31201 caaaatggcg tatttgccac ctggaggcgc tccgacggca ccgcctgttc cgccggctgc
    31261 tgccccgccg ccaccaccca tgccgccacc accgggaccg ggcggggaca tcgagtacat
    31321 gttgacgctg gggcgatcgt ggaccgcacc cggacgtccc gtcggaatgg cctctaaagg
    31381 aatgggaggg gcagacttgg gttgtgggtt cgccgacatc atgcgcagtg tatcaactcg
    31441 cctctcagcg ttctagcgct gtgatagcgc aagtggccaa agtcgatggg aggagccgcg
    31501 cagaccgggg ccgcagaagc gggatgactt acgtcgaatc ggcgggcgaa ccacccaaag
    31561 aagccccagg cgcagccagc gggcgacggg attcgctgcc atcgtcgttt ttgaccagca
    31621 gttcgatgcg ctgttcggtg gcgtccaaaa tggcgctgcc gcgacgcgaa agccgcacgc
    31681 cgtcttcaaa aagcgacagc gcttgttcca gcggtaagtt gccttgttcg atgcgggtaa
    31741 cgatcttgtg cagacgatcg agcacttgat caaagcccag ctcgcgctcg tccggtgggg
    31801 tcacagtctg ctgttcttcg gccacgtacc gcgctttccg tttctccccc cggagattgc
    31861 ttacaaaaag tccgctcccc gtcgagcgga gcgctgcgag ctagttggcg ctgccgaact
    31921 tgtaggtaaa ctgctccatc ttgccgctcg gtggcgctgg gaactgccac gtcgtcgctt
    31981 tggccttcac gcagtcggac agcatctggt ccttgtactc atctttggca aagccgaagc
    32041 ggttgacttt gccgtcgggg ccgatctcga aagccagctc catcatgccc ttgaggttcg
    32101 ggttgcgagc cgacgcttcc tgcagacagg acttcagatc gttgttgttc tgccgcaccg
    32161 tggcgatgcc ggtgtctttg taaccgtggc agccagccag cacgacgagg gaaaacgcaa
    32221 acagaccagg gatcgaacga gtgagtttca acataggtat cgactttgcc aagcgatcca
    32281 cgggacggtc aagggtgcag cgtgacaccg gcgcaggtct cataaaatgc acacaagcgc
    32341 gcggtcaggg cgggccaggt gtagcgcgac cgaacaaagt cgcggcagcg gttgctctgc
    32401 gctttttgca gggcttccgg ctgcgaaaag agcgcgcgtg tcgattcggc aaaggcccgc
    32461 ggatcggcat ccacctgcgc gccgattcgc tcgctggcaa gctgcggcca tggcgtctgt
    32521 gtggttgcga tgactggcgt gccttgcgag agcgcttcaa tgacgaccag cccgaagctt
    32581 tccatatggg aacacagcca cagaaccgcg gcagaagaca gcagctcggt ctttttcgtt
    32641 tcgtcgagaa ggcccggcca aaaaacagcc tgcgcaagtc cgcgcgatgc cgcctgctgt
    32701 tcgagccgcg cgcggtagcc gggatcttcc gtcgggccag caagaatgag ctgggcagtc
    32761 ggatgggtct tgtgcagctc ccacagcgcg tcgatcgcca gctccagtcg cttgaccgga
    32821 tgaatccgcc cgagcacaac aatctgcgga ttgcgcgcca gcggcggttt gtcggtggtg
    32881 gcaatggcgt cgggccagat tccatattcg acgcaggcga tcggcatccg cgcagaaaga
    32941 agtccatcgc tgcaaagcgc gcggatgccg tcggcctcgg cctgcgacga tacatgccag
    33001 ccgccgatcc gatcgatcag cgggcgcaga agccgcagcg ccgccatttt ctgggtccgt
    33061 ccgcgtccac tgtgcagcgc ccacggcatc agcgagccat gcggcgacaa gacgatcggt
    33121 cgacggtgta gcgccgccag cgacagcccg agcaggctcg tcggactcca cagccccgac
    33181 acatgcacca gatcggttgc cgccagcgcc tgcgcaatcc gcaccaccag actgggggcg
    33241 atgtctttgc cgattcgcac cggcgcataa aacgtcggga cgccaatgtc tgtgacccag
    33301 cggtttgaca gcgccgtcat ccgctgcgtc ccgtcggcat cactggtcag cacgcgcacg
    33361 tccaagccgg cttcgacttg ggcgcggcac agctcgaaaa gcgcccgcac cggtccgccg
    33421 tacccgaccg ccggataaaa cgacggcacc acatggagca cgcgcaggcg cggcttgggg
    33481 atcggctccg cgggtcggtt catcgctgcc cgcataccgc agtttgtcgt cgagcgcaaa
    33541 aacctgtgca ggcgctgcca cggcgggcgg gcgcttacac ccaagcggga tacttggcaa
    33601 atatttggaa gtctagctct caaaataggg attgtttttg taagaccgat gccgataaac
    33661 tggccaagtt aaatcgcgaa ggagctcgtt tggcatgacc agtctgcccg gtggcttacg
    33721 ctgcgaagac ctgatcgcct tcgatgtggt accgagtctg ctttcagcgg cgcagtccgc
    33781 tgggctttct ggtttgctac cgctgcaaga acaagcgatc cgggcggggc ttctgtcgtt
    33841 gccggatggc agcaggccgg aacaagacct gctctttatc gcagagccgg gatcgggcaa
    33901 gcggaccatc gccgagctgg ctgctgcgca ccatgcccac gccggacatc gcgtgctgct
    33961 ggtgacacac gatctggaat cggcgcgacg ctgcgtccag cgctttgcgg cctatgccgc
    34021 gattggtctg cggactggac ttttgcacga tccggcggac ttcgctgcgc tcgatctggc
    34081 ggttgcgccc ctggataccg cggcgctgct cttggccagc gacgaaccgg tgcgccgtgg
    34141 acttgggctg gttctggtcg aagaactaga acaagtggcg gactcggcgg atagcgagct
    34201 gtggccgctt ttgctgctgc gctgtcaggc acttcgggcc gacttgcccc tgcgcatgct
    34261 gcttttgtgc ggcgatgttc ggcttggtga aaagctggcg ctgctcttgc gggcgaaggt
    34321 ggtgcgcgct ggtcgacagc ttccgtcgga agaagatgct ccgtcgttga aaagagcgcg
    34381 ggcgcttttg caattgcggg gcggaatcgc ggcgcggcgg gacaatacgt gcactgcggt
    34441 gcatgtggcc cgaccgcgct aggtttgctt cgctgtcgct gtttggcagt cgttgacgga
    34501 gggctggggg ctctctaagc ttgacgtact ttgcgtcagg atgcctccat gaatgctgct
    34561 tccccggttg cgaccgcttc tccgagtccg acgagccttg tgacttccac cgaccagctc
    34621 gtcgagcatt ttcggcgcgg cgtgaaaact gggcccctgc gcatcggcct ggaacacgaa
    34681 aaaatcggtg tcgcgcttga tgaagacggc acggtgcgtc cggttccgta tgcggacaag
    34741 ccaggtcgtc cgcagattcg cgcgctgttt gctgggctgt gcgaaagctg cggctgtgag
    34801 gcggtgctgg aaggtggcaa caccatcgcg ctcaagcgca aagacggcgc gattacgctc
    34861 gaaccgggtg gtcagttcga gctgtcggcg cggccttttg tccttgatac cgagtgtgcc
    34921 gcggatctcg acgagcacct gcgcgagctt ttgccgctgg cggagcgctt gtcgatcgcg
    34981 tttgtcggcg tcggtttccg accgcttggc acctgggacg atgtgccgtg gatgcccaaa
    35041 ggccgctaca aagtgatgcg tgagtatctg ccgacgcgcg gcaagctggg cgtcgaaatg
    35101 atgaagcgca cagcgacggt gcaggccaac ctcgattatg tcagcgagga agacgcgatt
    35161 ctgaagatgc gggtgggcgt cgggctggga ccgctggtga cggcgctcta cgctgcgtcg
    35221 ccgctcgttg atggcaagcc gaccgactac aagagcttcc gggcgcactg ctggctcgac
    35281 accgacaacg atcgctgcgg cgtattgccg tttttgttcc gcgacggagc aagcttccgc
    35341 gattacgccg agtgggcgct cgatgtgccg atgttttttg tctatcggca
//
LOCUS       DAORGN010000023        34809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_450_length_34809_cov_5.812338, whole genome shotgun sequence.
ACCESSION   DAORGN010000023 DAORGN010000000
VERSION     DAORGN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 34809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34809
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_34809_cov_5.812338"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKL17_04500"
     CDS             complement(<1..865)
                     /locus_tag="PKL17_04500"
                     /inference="COORDINATES: protein motif:HMM:NF038073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HNK44020.1"
                     /translation="MNSGVQRPLEILWRGPLDSCNYSCDYCPFAKRAATKKVLEQDQR
                     SLARFVDWVKTETRYALRILFTPYGEALIWPSYRQALVDLSHIPHVEQVSIQTNGSGP
                     MDFLSAANLAKVSLWISWHPTEIQQIPFAEKICALHAQGVSLSVGAVAIPQHVEPIEA
                     LRNQIPSSIPMWLNAQKPGVRYSETELQRFRAIDPDFDVDRRRHRSFGKECSTGYDSI
                     SVDGAGEITRCHFAHTRLGNLYSDDLSTLLSPRPCPRRTCDCWIGYVHLTELGLADRF
                     PRPRRLARIRVP"
     gene            complement(865..2175)
                     /locus_tag="PKL17_04505"
     CDS             complement(865..2175)
                     /locus_tag="PKL17_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HNK44021.1"
                     /translation="MKERLSDLLSRGGYHSYAYSYPHKTAYRTLPQPLSLRDLWSTEN
                     RSALFLYLHVPFCEQRCGFCNLFTQVMPKADVVTRYLDTIDRQAQVMHDVLEPYRFAQ
                     LAIGGGTPTYLDAQQLARLFATVRRLAPQVIPTSIETSPATLDADKVSLLRAEGVSRI
                     SMGVQSIFSHETSAVQRRQQLADVDRALSLFYDAIPIRNIDLIYGLPNQTEASLRESI
                     SYVVSRGANELYLYPLYVRPLTILSRHKTHQDHRVSLYRDARKHLLTLGFRQVSMRMF
                     TRVAPQAQTDDAPRYRCQEDGMVGIGPGARSYTHKLHYAAPFAVGQAAIRDRIETWLS
                     QSNEDFSVARHGFLLGPKEQQRRYLLLSLLEGQVCRPSYVKRFGKDVLLHFPELSEAV
                     AAGLVCDTPSALLLTEQGQQYADVLGHWLQSEEVLAARSSWDAA"
     gene            complement(2172..2978)
                     /locus_tag="PKL17_04510"
     CDS             complement(2172..2978)
                     /locus_tag="PKL17_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HNK44022.1"
                     /translation="MVSFQAAFGKRDVVLLTLDALRFDVAHQAMQKAKLPNFAALLPQ
                     GFEARQTPGTFTFAAHQAFFAGFFPTPAHTAPQQRPMALRFPGSRTTGPTTLLLEGTS
                     IVAAFAQANYRTICIGGTGFFNPQTPLGTVLPALFQEAHFGRDMGVTSPVSAEVQFAL
                     AQKRLGELPDSQRVFLFVNVSATHPPTRIFHSGAKKESCETQTAALCHIDRHLPVLTA
                     ALKKRGGAVGIVCSDHGTCFGEAGDDGYVGHRIAHPNVLTVPYAEFEVSA"
     gene            complement(2990..4063)
                     /locus_tag="PKL17_04515"
     CDS             complement(2990..4063)
                     /locus_tag="PKL17_04515"
                     /inference="COORDINATES: protein motif:HMM:NF038074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4014 family protein"
                     /protein_id="HNK44023.1"
                     /translation="MSRGLVVVAQPGCKRLLGLLSALTKGHHPAPQLVSWDEALQSPQ
                     RLCTLGHSGDFLRVESPGADSHIWHQIARRGGLSGTFSDGQWRPGRAWFLGLSEILSA
                     WERQTQHLIWTHPAAHILTMTDKLACAQTLWAAQIPTPEVQLAATNPDAFREQLQRTG
                     RHAVFVKPRWGSSGAGVLAFRKSGAREQITTTAQLADGVLWNEKRLHVYHDRKSIDAL
                     LSAVLTDGAVVQRWIPKAITGGGPFDLRVLVIHGKIAQQVARVGRGTITNLHILAERQ
                     EVALALAKFPSSVLSDIHDICRRTAACFPDHHAIGIDVMVDVKGRPFVLECNAWGDYL
                     PRLLSDGKDSYESQVDALFSWAA"
     gene            complement(4063..5277)
                     /locus_tag="PKL17_04520"
     CDS             complement(4063..5277)
                     /locus_tag="PKL17_04520"
                     /inference="COORDINATES: protein motif:HMM:NF038076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4015 family protein"
                     /protein_id="HNK44024.1"
                     /translation="MTNHTRRFEMVEGASSKFWQISIEGSSFTVTYGRIGTAGTSKTT
                     ACTSPEAAKTEADKLIAEKTRKGYIEQGGSAPKDTFRPPVEIDIMDHAARYLNYKVTN
                     FVVGDGGDGDDEGGRSYGALRDLDKRVFRVGISYDDDAADFTARLDALLADKKLGELR
                     GLVIGQWYGDYCDKGPNAMCEKLLANAAKLTSLKGLFVGDIVQEESEVSWIHQCDYGP
                     LLSALPQLEDLVIRGGENLRFSKLKNSTLRSILLQTGGLSKKTLKDLFSAELPSLQTL
                     RLWLGVDDYGGDGTVKDLAPILSGKLFPQLTYLGLQDCKYSDDIAKAVAKAPILERLK
                     GLDLSMGTLSDEGAKVLIDTPAIRNLKYLNLRHHYMSKEMVKKIRELGIEVNVGDQRT
                     ARDDERYPEVTE"
     gene            5469..5900
                     /locus_tag="PKL17_04525"
     CDS             5469..5900
                     /locus_tag="PKL17_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44025.1"
                     /translation="MASLQKLSWRAIQGVAGVLGCMYLVSGYIFITTRSIEFVHYTGV
                     SAQEVWTIRNLGVRVLAIGIGLLLALLMRRKDALALMLLVRLCADVGDFTNSALTPGL
                     DASVARTLAVFCVVELVALSGLVRILSAERKNAAATDGKAV"
     gene            5903..6262
                     /locus_tag="PKL17_04530"
     CDS             5903..6262
                     /locus_tag="PKL17_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44026.1"
                     /translation="MNRKIPAWVSVLVSITVAILLGKSIAIFRGPLLPREIGLQLVEL
                     AGRNAAMLVASLLALVTQRREYFVLLCVMGVCRESWDFGNVVYFDGFRPGSLGLLAFV
                     GVWLAALWTLRNRPVGA"
     gene            complement(6299..7900)
                     /locus_tag="PKL17_04535"
     CDS             complement(6299..7900)
                     /locus_tag="PKL17_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44027.1"
                     /translation="MAAPRVSQLLASRLIRRQPVRRPSEGLGQPPQIVAPDPRTLEIT
                     QLLALPSGPERTYKLDAYFFFPPSFGISDTSYSQSDFYGDASIRMRLHSPTVLLADLA
                     DLNSPRNPGTMLRQQLPLLLTDDAPRASALVKLAQLYSAEVADAASTEVEVLRQLISQ
                     AKASPIDRKRLVENLERATSHMLKALGSMHRLRAKVAAFAPLQPKEVPQALAFAEEYV
                     SAVIDLKLAELAELIDGLPELRDGTSTATRMRVRLGRTIEQVNRYRLDQGFATPWGDA
                     PEYYSYRIGRLKSILERALYIDTRQADRDPFYRNSAAMVAAGLAATWATLAQVPLLNY
                     DLARDHQGLLLTFAVVAYVLKDRIKEWTRQSLSSKILRWDHDRKLIFDTLEEVGFGSI
                     AGRARERVHFLPESQVPQDVMALRVLNRTVRGVTTETEEILHYHRELTLGAGSSPTPA
                     GFAVQDLLRLSLADVLARLDDPVAQVRFYDYQTGRFRSAQIPQVYHLNVLLASLDTVS
                     GKRTLSRTRVVCTRKGIIRLDHYGD"
     gene            complement(7882..9390)
                     /locus_tag="PKL17_04540"
     CDS             complement(7882..9390)
                     /locus_tag="PKL17_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNK44028.1"
                     /translation="MLRIPLPVKLLISYLGVLLLGAGPTFFYIRVVLQGDLLRESALR
                     MATQSQKVAELLRSVPEADRLPLITKFGEMSTPRLTYMSLAGDVLLDTSVVRPLSINH
                     ASRPEVRMAMGDTTSKPEGLPVLSGLLGLGYARRHSDTTGIETLYVARQLVDAKNQPY
                     GVLRLAMPVDSIEAVTSGTMRFLLNAMAAATSVAIVFSLISAILFVRPLQRVSQMAQS
                     LASGDLGVKVLQPTNDEVGDVARGLNQMATALRRRLLSAGMSEALLSQLVDALPCACV
                     IFEENGQMIACNGAGRRALQLSGPEAGARMNEFAEHPQVQSILKLAENDGQAEPVSLE
                     LIEGQLIQGLLHVLKRPGMAPLRVFIGVAQPAIEASLLPAVEDIVPCLLSDVLSQAEK
                     EARSGLLENGHRLEVTWEGIGGISPQLMVTDAEGRLASTISEILSAVVEATPSLGEQL
                     SVGVSQEATRIRLHIDCGVPSHVVSVVRPLIDPLGGEIETSPLETNLWLPRA"
     gene            9507..9580
                     /locus_tag="PKL17_04545"
     tRNA            9507..9580
                     /locus_tag="PKL17_04545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9541..9543,aa:Arg,seq:tcg)
     gene            9668..10663
                     /locus_tag="PKL17_04550"
     CDS             9668..10663
                     /locus_tag="PKL17_04550"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNK44029.1"
                     /translation="MFEGFANVWTPVTLASRLGKTPLAVELAGEKLVFFRGRDGKVGA
                     LVDRCPHRGVRLSLGSVAPSGCLTCPFHGWEFDVDGNARFVPLNPDAKLDRLSATALP
                     ARELGGLLWVYTAPKSTPAEPIVPATLTDPKLARTYLEEEWNAHWTRAMENMLDSPHV
                     PFVHRNTIGRFVRPRLQPNSRMDVEWEDTDFGGRTFAIIDNDRSIDPGRLEFYRPNIM
                     VLHIPMPKQTFRMHAICVPVAAQKVRMIVVGARSFAKLSLLNPLFNYSNKRIVAEDRA
                     VVESSDPPVVPPAAQEVSVRSDRATLAFRKYYFQSLHTSSVSVKRGLPVVTDSPV"
     gene            10883..12292
                     /locus_tag="PKL17_04555"
     CDS             10883..12292
                     /locus_tag="PKL17_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44030.1"
                     /translation="MHRRTPLWLPLFGLLAAPALGFADTAAPPVSASHGRAVASFWGI
                     RQDCFFDGMIADAVASRAVIGSPIRALSVPRNAATMGCFGKACVEEIRKESACADLDL
                     SGGVIGGEAAEFVEQGQRVYKLRLWKYLPATDSVLEHEALCRSDGFSSRADLARCAAE
                     KVVELASEHFKAPVHLVDTPECKPLSVTPAPVVPSPVAAAEKVPVYYAVTYSSKNEMG
                     TAKSAREHLEKRFSSEHNLVRRSSKANALSAQGSAPSVETAAEYLRAVPATESAAAPL
                     LLLTHVHAKNSTCGTWFRRAPDGNIQKFGETCGPIEVLDALPLPDSNIESIPVVPAEP
                     KVATRAKTPDFCRASIPQCPSSFPFATVLPKHGGSVGKSLLSRGPGWIPIIGAGAGLV
                     TMIALGVEDRNYRLSWPENPGWQSPEHLLASAFWTATGLTIALGASAAAVVVDHVQHK
                     HSGSAASPQACSFASEVQP"
     gene            12289..12981
                     /locus_tag="PKL17_04560"
     CDS             12289..12981
                     /locus_tag="PKL17_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44031.1"
                     /translation="MKSKPTLLRLCGLLALSLAPACIPSGATLPSGILTADGGSDVPD
                     MAMPPVVRKCKDFDAFAGSELTCWDFTDDATRASVNDWAALQDVVTKSCWSASSTTPT
                     VLQGGNASLPAKVECSLALTGNSSISGKGLDLGGVSSVTLAMSFQVAGFANPPTQSAF
                     FVRPGLLPEIPLGTIGGAPKDFQSSALSFPVSIDQSANFQSSDLKLTFARSLLDQQIF
                     IRSIAVLGTPRR"
     gene            13051..14622
                     /locus_tag="PKL17_04565"
     CDS             13051..14622
                     /locus_tag="PKL17_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNK44032.1"
                     /translation="MKLTPFAKLFIALVIVAATAYVVKVNVYDPARQAVELAAQRHTK
                     GGDVEPAKPVETAAASAQTAGPDRPIVIGINDFGGGYPLLLANDGAKPGPQSLFRQAG
                     LDVEVRLIRGSKERLKAFDDGEVDVMLLTLDYFANLAPLYKQKGVELRSFLFVDWSRG
                     NIGIAAKPQWTSIEALRTARIATTRNTPTHYFLLSLLEKSNLKPAEIDTIKSSLVFAT
                     KTPDAGALFQRGEVDAVAIWEPHLSEAIAGGKGSTLVTTETATNLVGDVLFARKDWLE
                     KNRDKVVALSKAYFSAVDAMNKDPQHAIELSAAAFAQKPEQIAANVKKVKPAQFADNR
                     AFFGLETEDCAYDQLFVEASGFWQREGLFKEPTPPAQSKWLRALEALAPQYKEQKVAE
                     SFHFQSLPKAAAPSLLTKSVSIYFASGQATVDQNARKVLDGFAQTLAVFQNAYVQVEG
                     NTDNVGARPSNVALSKQRAESVVAYLVDRHRLDRSRFIAIGNGPDAPVADNKTPEGRE
                     LNRRTDFKIIKNATP"
     gene            complement(14626..15126)
                     /locus_tag="PKL17_04570"
     CDS             complement(14626..15126)
                     /locus_tag="PKL17_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNK44033.1"
                     /translation="MSENGCVVWLTGYPASGKSTLARAVHQKLMEQGRAACVLDGDDV
                     RAVIRPSYGYDDQARTDFYASLAGLAALLAKQGLIVLVPATANRQKYRDTARSLAPHF
                     VEVFVDTPLAECQRRDPKGLYKQAPSTLPGSGTSYEVPEHPDLTVRVGDKGVENVLAL
                     LLQNGT"
     gene            complement(15123..16223)
                     /locus_tag="PKL17_04575"
     CDS             complement(15123..16223)
                     /locus_tag="PKL17_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNK44034.1"
                     /translation="MSPSSSSPTALARHVLSLYPESRIKRISALGTDDASDETTKGLG
                     YGRPVRVDLEGPDGPRRLVFHTARPDDFGHDRRSDRAQAMLLLWDSADRIPFHTRAVD
                     VGALRQSGDLISLRDAGEFYVVTEWCDGNPYADDLRRVAMTGHATPTDLERAEVLARA
                     LIEIHAAPGSHPAAYTRAIRDLLGHGEAIFGLVDSYPATTPGIELDELRRIEEKCLSW
                     RWKLRGQTDRLRKTHGDFHPFNILFDDAGKLHLLDTSRGSEGDPADDVACLAINYLFF
                     GLEHRRIWEHGLGRLWDRFWQVYLAESEQIAAPNVLHAVAPYFAWRGLVLASPLWYPN
                     LAAADRRRILAFVFRVLDAAQFDPAWGHEAMQ"
     gene            complement(16255..16935)
                     /locus_tag="PKL17_04580"
     CDS             complement(16255..16935)
                     /locus_tag="PKL17_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44035.1"
                     /translation="MLRQLLLLSVSLGLLVTAPELALARARKSSSHASSSGQRKHASS
                     KRKKKKQKHYRPPEIPQSLELTQFDLVTGQGQLIVVGTKHVPEIRLLVLTDDRGRRFV
                     PDMADCQPPVGVSLPADGSDPALVPANLRWRCTFSVPLLYRRAALVGVAMEWGDKVVE
                     VPAKKVSFVYAAAAKSVPSAPLPREDGVIAPRPVQRPDGEDGGGDAEDSEILDEEVSD
                     SEDNQRER"
     gene            complement(16965..18284)
                     /locus_tag="PKL17_04585"
     CDS             complement(16965..18284)
                     /locus_tag="PKL17_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNK44036.1"
                     /translation="MAISRGSEIKPAQGKLGVLMPGMGAVSTTTIAGVEAVRRGLAEP
                     IGSLTQLGTIRIGKRTENRTPKIKDFVPLADLNDLVFGGWDIFADDAFEAASHAGVLN
                     KDTLGQIQPYLQSVRPMAGVFEPHWVKKLHGTHIKKGTSKRDLAEQLIADIANFKKQN
                     DLSRCVAVWCGSTEIYQTPTHVHSSLAAFEAGLQSSDEAIAPSAIYAYACLKSGVPYA
                     NGAPNLSLDFPAMHELAKQQGLPIAGKDFKTGQTLMKTVIAPMLKARMLGLQGWYSTN
                     ILGNRDGEVLDDPESFKTKEMSKLGALEYILQPHLYPQLYKDFHHVVRINYYPPRGDN
                     KEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPIVLDLALFLDLASRSGMSGVQEWLS
                     FYFKTPMTAPGLYPEHDLFIQLMKLKNTLRYLRGEELITHLGTEYYD"
     gene            complement(18292..19599)
                     /gene="hflX"
                     /locus_tag="PKL17_04590"
     CDS             complement(18292..19599)
                     /gene="hflX"
                     /locus_tag="PKL17_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNK44037.1"
                     /translation="MSNSSSDVPDLLQLLPKRAILCAVQLPEEDELSFRESLGELTRL
                     ASTLGMQVEGEITQKRGSFDSAAYLGPGKLEDLAQLCKREEVPTAILIDHEVSPSQAR
                     NVQKATGVDLVLDRTAVILEIFHRHAKSRQARAQVELVRLEYMAPRLRETQGMGDRVR
                     GGIGGKGAGESQLELDRRKLRDRVAELRDELVALEREHKTRSARRQGLRRVALCGYTN
                     AGKSTLFRSLTGAEVYVADKLFATLDTTVRQLHPEPRRTILMSDTVGFIRNLPTKLVA
                     SFKTTLDEALEAGLILHVVDASDPAALRHVDTTCGVLAEIGAAEVPQLIVFNKTDRLE
                     DGKVPDEWTARFPRQVSISAKNPDDVARVRQLIIDFFDEGLIETELEVRYDQPQLRAQ
                     IFATTEVLHEEFGEQSAKLRIKADQATLGRLARNMQEHEGLKE"
     gene            19816..21576
                     /locus_tag="PKL17_04595"
     CDS             19816..21576
                     /locus_tag="PKL17_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44038.1"
                     /translation="MTSVLITDGHELAGLGAARSLGRAGHFVSVAVPQGIDAPVGKSR
                     YVARVIASPNPWADQPAYRQFLQSEAPKHDVLLPISEAAILATAACRHNLGRTLLLLP
                     SDASLRYTLSKYHATRAAQAAGLAVPPTVFLSDGNWPADLLPLVDSLAGLGYPLLIKH
                     DNCLLPSGQYVRGKTERVDSPLQARALLAELRALGAQVIAQKPIPGHGAGVFLLRHGQ
                     KTLLRFAHERLHEVPYTGGVSSLRMSCHDEKLVAQSERFLAALDYQGVAMCEFRKTPS
                     GEPLFLEINGRLWGSLALALHCGVDFPRTLLLAMQGMPVPAQADYPDGLRCRNLLPGE
                     VSHLASLWRAKDVSLSDKLVAAAKQVLLTFDPQIRHDHFWFDDPEPAAKQTQNWLSEF
                     AQRLRGRVERNRQMQSDKALLSEELARTESRFGALPTPRRILTLCLGNICRSPFAEHY
                     LRLSAMQKGLPMLVVESAGFLHHDGRATPTRFVELVKPHGVDLSHHRAQRVQREQIDA
                     ADLILVMDVQNLRAFHDEFPDAVGKTFLIGALAKSHSPEIPDPYLLPVREAEASYRLL
                     AESCAAVITRIVHKSTLRRL"
     gene            21592..22491
                     /locus_tag="PKL17_04600"
     CDS             21592..22491
                     /locus_tag="PKL17_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK44039.1"
                     /translation="MNIRRLCGWCALVPLGLLLGCGFTRQHAAKSQVDVVEDQPYVSA
                     SPHPKHRLDLYLPKVRHNVPLVLFVHGGFWKNQDRRYYQPFTGLHGNVGMALAQHGFA
                     VGVTSYRLFPSANIDMQLADVSAAAQWMVDHAKDWDGRSDALFLVGFSAGGHLVMSLC
                     ADPARLSASGIPVAALRGCASLSGVLDIPALAAQKDDAFNRELTYPLFGNTTESQAAY
                     SVFRTISPSLPPIFLLWAEKDLPFVKTASLHALQKLQQAHKEVDSFELSGYDHSDVVL
                     RILTDRDDLTPKLVRFLSAHSGP"
     gene            complement(22526..23881)
                     /locus_tag="PKL17_04605"
     CDS             complement(22526..23881)
                     /locus_tag="PKL17_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44040.1"
                     /translation="MTRSILATWSLVLVFLSAGCSVDRPPLDDSSPTDRSDGFRPGGD
                     MQIPPPVCKGRFCDVPKCPPGTTTEVVGKLYAGNGKDPIPGASVFVPVDNLPEFPPGL
                     GCDLCNDTPPSVTSAITDFDGSFRLRGTPAGKAFPVVARLGRFQRVVSVEATACVENT
                     VPADAGSKGIRFSKKNRELSPQDYIPRIAVVSGDYDQIECVLKRIGIDELDMYNGRAV
                     GMKNPPSIAEMGTLLSDENKLLSYNIVIVNCSDNQYQSLLAAPQVKKNLEKFVRSGGR
                     LYVTDWAYDVIEQVPEFSPYLCFEPQSIPGPAMCLLAPEKAMAADSRSYYGGQYRVLD
                     KDMEKWLSQMPGVINRNSQVAVDYSFVVVSKASDDPKTKTKVWVDGPASGFGNRPQTV
                     TFDYNACGRVHFSTYNTEPDGVVDETARWPSACKVDFSPQERLLEYLFFNIASCVQGP
                     G"
     gene            23933..24583
                     /locus_tag="PKL17_04610"
     CDS             23933..24583
                     /locus_tag="PKL17_04610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44041.1"
                     /translation="MVGTLSARISAVNRRDLFATVFAFCFVADVGCETRRGPADHSAA
                     TGASELSASQLAAQDSFRRPDRLLLALGIARGQTVAEIGAGGGYLTRHLAQAVGPSGK
                     VVATDIDKAALAALRSRMQAAFQVETRLVPRDASGLELSRYDVIVLADVVHLLPQPDR
                     YLPALFPSLRPGGHLYVVGREDRMPGLAQAAKRAGLQIRPVQADLPAQFVIELLLP"
     gene            24627..26216
                     /locus_tag="PKL17_04615"
     CDS             24627..26216
                     /locus_tag="PKL17_04615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44042.1"
                     /translation="MRDAVPPTEYDAVPYPCGGHDYTHPGHLAMLATLFGLSAPPVAT
                     CRVLELGCGDGSNLIPMALAMPNATFVGIDLSARHIETGQQEVSQLGISNLSLRHQDL
                     LTFGDGEQPFDYIIGHGIYSWVPEAVRSKIWDICSNLLSPHGVAYLSYNTYPGWHQRK
                     MVRDMMRFHTQRLDDPDRRRAQALAMVNFIAGAVPSQFPLHRKALETELARLTELPRN
                     YILHDDLGEINQPYYFHEFITAATAHGLQFLSETRFHVMQDNRLSPEARNTLRQVGDL
                     LVMEQYRDYITCSAFRETLICHATRSLERRIQPSQMERFSFQSSLRSQSAQPDVVHAV
                     DEKFQSDGMTLSLSEPIAKAALVIMQQNAPQAIPFETLFADSLRLCRADSQADFDLRF
                     RMNQRQELSELLLAGFGAGVIRPTLFQAPFCLRPGDKPQLEPLARLRMSANKEVTTPL
                     LDNIRFDEPFGRAVALLCTGQRSRSDMVTELSQRIAAGEFPSELSGGSVPSPEALRTM
                     VESRLTEVLDRIARYGILVEN"
     gene            26258..28576
                     /locus_tag="PKL17_04620"
     CDS             26258..28576
                     /locus_tag="PKL17_04620"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNK44043.1"
                     /translation="MDRRLGVFASVLVAFATLSLSACDGPQVDPPPIDTSVGSGPSRY
                     VQVFVGSDDAEGVADPVTNGAGGSTFPAAAAPFGMVQFGPDTPWAIPPGYKYSDDQIV
                     GFSVNHLNGAGCPAQRDFPLTPMVGDVDPEATLPFSHSQEVASPGLYEVRLASGVTVD
                     LTATPRTGMARFRFPKTSAAHLLLRSAMPWDLLVAKDASLQVVSSTLVTGWRSDAFCA
                     MGVPTKIYFAARFDRPFSARSFGTKDETQTHAEGIRSGLVLSFSSEQNPIVHVKIGLS
                     SVSIDGALRNLDAENPDWDFAALRKKTLASWDDYLGRLAVSGGSEELLTMFYSALYRV
                     FLQPSVWSDVDGSYVGFDQNIHASDGHVHYANFSGWDIYRSWIQLVAWLAPKETSDIV
                     RSLILDGQQGGALPKWAYGASETGVMIGDPADALIANAWAFGAQDFDPAAALALMLHG
                     AKDPTATCNELRVRPGLSDCLDRHYCPVDGPDGVSGSVAATLEYAIADAALGQFAAMQ
                     GDAKTAREFADRSRYWRNVFDPRAKAGSAVGLLQPRRRADVAGAADFVVAKYDDGKGV
                     VEGSLEQYSFMVPQDVPGLIDAMGGDAAFVARLDEHLQKVNAGTKSPHFYIGNEPGFA
                     TPWLYPFAGSPWKTQEAVHRIVTRSFSATPRGLPGNEDLGALSSWLVFAMLGIYPEAP
                     GVPGFVVGSPFFPQIAVKRPGQADFHISAPGLSDPVYFIVDATLQGRPLRVPWLTLDQ
                     MKAGGELTLTVSSRPNLSWGAAPADRPQTQLP"
     gene            28588..29448
                     /locus_tag="PKL17_04625"
     CDS             28588..29448
                     /locus_tag="PKL17_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK44044.1"
                     /translation="MESSSHYLLCRGRELHYLDFGPQDAPVVVAWHGLARNAHDMDVV
                     AQHLAGRFRVICPDTIGRGLSAWSPDPAREYCLDFYVELAESLLDTLGMSRCLWLGTS
                     MGGAIGLRAAAGRLRGRIDRLILNDIGPEISAAAVERIRSYAGQPPSFATVRELAQYF
                     RTIYAPFGTLSDAQWLHLTETSLRRLPDGRVTPHYDPQIVQQFIVHPHDYAQWPAWDS
                     LSLPVLCLRGERSDLLLPEVTEQMKRRGPRADVITIPGCGHAPALHVPEQLALIDEFF
                     SAKLVRSGGG"
     gene            complement(29445..29906)
                     /locus_tag="PKL17_04630"
     CDS             complement(29445..29906)
                     /locus_tag="PKL17_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HNK44045.1"
                     /translation="MSRQHRGRKGSEADEPFSLDSGESLGSRVVRLQHLMFEELYRLF
                     RLEVSDPRLQDVVPTSLQLSPDLRNAKVMFAIQKPPEEAKDVKAKKTPGEARIAVEAK
                     LRTIHEALGKVTPFLRVRLADAVQMKRLPDLHFHRDRLAESSLRATEIRTD"
     gene            29963..30367
                     /locus_tag="PKL17_04635"
     CDS             29963..30367
                     /locus_tag="PKL17_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNK44046.1"
                     /translation="MKFPDDANVGAEKKALLRDRLQRFSIDLSAVEEKAVKGSGPGGQ
                     KMNKTSSGVLLRYVLLGQPILVKWTRERQHSLNRFLALRELCDEVEAIVSPETSERLR
                     ERERIRKQKDRVRRRSSDPKADPPADKSTSKQ"
     gene            30382..30726
                     /locus_tag="PKL17_04640"
     CDS             30382..30726
                     /locus_tag="PKL17_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44047.1"
                     /translation="MRKDVWLLVALLCGACPGGAQVKSGDFAVSERLRDEDCLTASEL
                     YKQRLAVPRRAGSIVSSRLTVRSEEEQAADLFGFVGKCQTQLAGRARRAIVRCWQDST
                     DAETFDSCNDRF"
     gene            30770..31795
                     /locus_tag="PKL17_04645"
     CDS             30770..31795
                     /locus_tag="PKL17_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNK44048.1"
                     /translation="MSSETMQAVVLEKTPEGPGLVYRSVPRPTPGPGQAQVSLHAAAL
                     NRRDVWIRLGRYAQIKLPSILGSDGAGVVSSVGEGVSPQWIGKSVVLNPALTDNPSPD
                     IQGSDYRILGMPEDGTYAESIVVSARRLVVMPAHLSFVEAAALPLAGVTAYRSLCVRG
                     QVRPGQTVLIPGIGSGVSTLTLLFALHLGAKVVVTSGSHEKLAMARQLGASFGVSYHD
                     ADWDDQIAKFLGGANVDVVVDGAGADAWAKSLKLLRPGGRLVSYGATAGLPQIDLRKV
                     FWRQLQILGSTMGNESEFAEMVALVAAGKLRPLIDQVYPLASAQQAHLRMEHSQHMGK
                     LVLQIRD"
     gene            31795..32691
                     /locus_tag="PKL17_04650"
     CDS             31795..32691
                     /locus_tag="PKL17_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNK44049.1"
                     /translation="MLDLLAAIERYLDVLRVERGLSAHTLSAYARDLQSFTDYLAEHD
                     PQAATDVTALSARHGLGFAVSLGQRQLALRSQARMLSSLRGLGKFLRRENLVPSDPMA
                     EVALPKVGRPLPHSVGQTDIEALLARPDVQTPRGARDAAMIEVLYSTGLRVSELCKLA
                     IADVGPGFVRTVGKGSKTRIVPLGERAQAAIAHYVQFARPTLLGQRQSPSLFVTHHGR
                     AMTRQGFHKLLAAYGRAVGIADLHPHLLRHSFATHLLDHGADLRAVQTMLGHSDVSTT
                     EIYTHVASASLRRTYRRHHPRA"
     gene            32901..33377
                     /locus_tag="PKL17_04655"
     CDS             32901..33377
                     /locus_tag="PKL17_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44050.1"
                     /translation="MSDCAFNPRAAFEQARLGTPFASLPIRGGIRAGDASTFRCHTKA
                     TFSPGTVRVYDVADHIVAWLDGTEPTSWTEEGSVHCDGGNGVILSAEALAEIEALEDE
                     FEMFDRIDDVDLPAQVTTPGGLPIAAFAFLGDGVYCTAIGCDEAGQPCALVVHDPN"
     gene            33524..34582
                     /locus_tag="PKL17_04660"
     CDS             33524..34582
                     /locus_tag="PKL17_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNK44051.1"
                     /translation="MAIYALWNNKGGVGKSYLTFQLACEYARTHPTQNVLVIDVCPQA
                     NASSMLLGGMRNGEMELDKLGSANPRLTIAGYIRERISSPYHNPAKGANYLLQVQQVN
                     KAVPPNLYLIPGDEELEVLASRVTNATSPGPDDAWSKVHLWLSDLIQDVRVRWNVSAI
                     TVFIDCNPSFGIYTELAMSASDRLIIPFSADGSSRRAVRSVLALLYGVTRAPGGQQSE
                     YFIKSQRFRLAVPKIYCYVGNRLTQANFASANAFKTVVDAIGNEIWQIWINNPGNFYV
                     HPTGEPPPSTRSAFKRMFQYEVVDANTASVVSQALGIPIVTLTAGSKRLVGKTAIVNQ
                     TQLNKQQPNIKALVATIE"
     gene            34588..34698
                     /locus_tag="PKL17_04665"
                     /pseudo
     CDS             34588..34698
                     /locus_tag="PKL17_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
BASE COUNT         6716 a        10229 c        10521 g         7343 t
ORIGIN      
        1 gcggcacacg gattcgtgcc agtcgccgtg gccgtgggaa tcgatccgca agtcccagct
       61 cggtgaggtg gacgtaaccg atccagcagt cacaggtgcg gcgcgggcag ggacgtgggc
      121 tgagcagcgt agacagatcg tccgaataaa gattcccaag gcgagtatgc gcaaagtgac
      181 agcgggtgat ttcgccggcg ccatcgacgc tgatgctgtc atatccggtg ctgcattctt
      241 tgccgaagct gcggtggcgg cgtcgatcaa cgtcgaagtc cggatcgatg gcgcggaagc
      301 gctgcagttc cgtttccgaa taccggacac cgggtttctg cgcgtttaac cacatcggaa
      361 tgctggacgg aatctggttc cggagcgctt cgattggttc cacatgctgg ggaatggcca
      421 ccgcgccgac acttagcgaa acgccttgcg cgtggagtgc acagattttt tcggcaaacg
      481 gaatctgctg aatttccgtc ggatgccacg aaatccacag cgaaactttg gccagatttg
      541 ccgcggacaa aaaatccatc ggacccgatc cgttggtctg gatgctgacc tgttcgacgt
      601 gcggaatgtg cgaaagatcc accagggctt gccgatagct gggccagatc agcgcttcgc
      661 catacggagt aaacagaatc cgcagtgcat agcgcgtctc tgttttcacc cagtcgacaa
      721 atcgggcaag ggatcgctga tcctgttcca gtactttttt cgtcgctgcg cgcttggcaa
      781 acggacaata atcacaggaa taattgcagc tatcgagcgg tccgcgccac aggatttcaa
      841 gtggtctttg cacaccgctg ttcatcaggc cgcatcccag cttgatcgcg ccgcgaggac
      901 ttcttccgat tgcagccagt gtcccaagac atcggcgtac tgctggcctt gttcggtcaa
      961 aaggagtgcg gaaggagtgt cacaaaccag tcccgcagcg acggcttcgc tgagctcggg
     1021 aaaatgaagc agaacgtctt tcccaaaccg tttcacatac gatggccgac agacttgccc
     1081 ttccaaaaga gacagcagca gatagcgcct ttgctgttcc tttggaccca aaagaaagcc
     1141 gtggcgagcg accgaaaagt cttcgtttga ctgggacagc caggtttcga tgcggtcgcg
     1201 aatcgctgct tgtccgacgg caaatggcgc tgcgtaatga agcttgtggg tatacgaacg
     1261 cgcccccgga ccgattccta ccatgccgtc ttcttggcag cgatagcgtg gtgcatcgtc
     1321 tgtttgggcc tgtggcgcga cccgcgtgaa catacgcatc gacacctgac gaaaccccaa
     1381 tgtaagcaaa tgctttcttg catcgcgata gagcgagaca cggtgatctt ggtgggtttt
     1441 gtgacggctt aagatcgtca gcggtcgcac gtagagcgga tacagataca gctcgttggc
     1501 tccgcgcgac acaacgtaag aaatcgactc acgcaggctc gcttcggtct gatttgggag
     1561 tccgtagatc aggtcgatgt tgcgaatggg aatcgcatca tagaaaagcg aaagtgcgcg
     1621 atccacatcg gcgagctgct gacggcgctg cacagcagat gtctcatggg aaaagatgct
     1681 ctggacgccc atgctgatac gggaaacacc ctctgcccgc agcaaggaca ccttgtccgc
     1741 atccagcgta gcaggggatg tctcgatcga tgtcggaatc acttgggggg ccagtctgcg
     1801 caccgtcgca aacagccgcg caagctgctg ggcgtccagg taggtcgggg ttccgccgcc
     1861 gatggcaagc tgggcaaagc gatacggctc tagtacatcg tgcatcacct gggcctgccg
     1921 atcgattgta tcgagatacc ttgtcaccac atcggctttg ggcatgacct gggtaaacag
     1981 gttgcagaaa ccacagcgct gctcgcaaaa cggaacgtgt agatacagaa acagagccga
     2041 gcgattttcc gtcgaccaca gatcgcgaag tgacaacggc tggggcagcg ttcggtaggc
     2101 ggttttgtgc ggataggaat aggcatagga atggtatccg ccgcgcgaaa gtagatcgga
     2161 aagtcgttcc ttcacgcact cacctcgaat tctgcataag gaaccgtcag cacattggga
     2221 tgggcgatgc gatgaccaac gtatccgtcg tcaccggctt ccccaaaaca ggttccgtgg
     2281 tcactgcaaa cgattccgac cgcaccaccg cgtttcttta gcgcagcggt caacaccggc
     2341 aagtggcgat cgatgtgaca gagtgccgcg gtttgggttt cacagctttc ctttttggca
     2401 ccagaatgga aaattctcgt tggtggatgc gtcgcagata cattcacaaa cagaaacacc
     2461 cgctgactgt ccggcagctc gccgagccgt ttttgcgcca gcgcaaactg gacttccgcc
     2521 gacaccggac ttgtgactcc catgtcgcga ccaaagtgcg cttcctgaaa cagcgctggc
     2581 aacaccgtcc caagcggcgt ctgtggattg aaaaatccgg tgccgccaat gcagatggtg
     2641 cggtagttgg cctgcgcaaa cgccgccaca atcgaagtcc cttccagcaa aagtgtcgtc
     2701 ggtccggtgg tgcgggaacc cggaaaacgc agcgccatcg gtcgctgttg cggcgcggtg
     2761 tgggctggtg ttgggaaaaa gcccgcgaag aatgcttgat gggccgcaaa cgtgaacgta
     2821 cccggagtct gtctcgcctc gaatccttgc gggaggagtg cggcgaagtt tggcagtttg
     2881 gctttctgca tcgcttgatg tgcaacatca aaacgcagcg catccagcgt cagcagcacc
     2941 acatcccgtt tcccaaacgc tgcttggaaa gacaccacgt tacactccat tacgcggccc
     3001 aagaaaagag tgcatcgact tggctttcgt agctgtcttt tccgtcggaa agaaggcgcg
     3061 gtaagtagtc cccccatgcg ttacattcca gcacaaacgg acgacctttc acatccacca
     3121 tcacgtcgat tccgatggcg tgatggtctg gaaaacaggc agcagtccgt cggcagatgt
     3181 cgtgtatgtc ggacagtacg gacgacggaa atttcgccaa tgccagcgcg acttcttgcc
     3241 gctcggctaa aatgtgcagg ttggtgatcg tgccgcgacc gacccgcgcc acctgctgcg
     3301 caatctttcc gtgtatcacc aagacgcgca gatcaaacgg accgcctcct gtgatggctt
     3361 tgggaatcca gcgctggaca acggcaccgt cggtcagcac ggcagagagc agcgcatcta
     3421 tcgacttccg atcgtggtac acatgcagcc gtttttcgtt ccacagaact ccgtccgcaa
     3481 gctgtgctgt ggttgtaatc tgttcgcgcg cccccgattt ccggaatgcc aacaccccag
     3541 cccctgatga tccccagcgc ggcttcacaa acacggcatg gcgacctgtg cgctgaagct
     3601 gttcacggaa tgcatccggg ttcgtcgctg cgagctggac ttcaggagtc ggaatctggg
     3661 ccgcccaaag tgtttgggca catgccagct tgtctgtcat cgtcaagatg tgcgcggcag
     3721 gatgcgtcca gataaggtgc tgcgtttgtc gttcccacgc ggaaaggatt tcggaaagac
     3781 ccaaaaacca cgccctgccg ggtcgccact gtccgtcgct gaatgtgcct gaaagaccgc
     3841 ctcgacgcgc aatctgatgc cagatgtggg aatccgcccc cggtgattcg acgcgcaaaa
     3901 aatcaccgct gtgaccaagc gtgcagagcc gctgcggact ttgcagcgct tcatcccaag
     3961 acaccagctg cggggccgga tgatgtcctt tggtcagcgc ggacagaagt cccaaaagac
     4021 gcttacaacc tggctgtgcg acgacgacga ggcctcttga cactattctg tcacctcggg
     4081 gtagcgttca tcatcacgcg ccgtccgctg atcgccgacg tttacctcga tccccagctc
     4141 gcggatcttt ttgaccatct ctttggacat atagtggtga cgcaggttca ggtacttcag
     4201 attgcggatg gccggcgtat cgatcagcac ctttgctcct tcatcggaca gcgttcccat
     4261 cgacagatcg agccccttga gccgttccag aatcggtgct ttggccacag cctttgcgat
     4321 gtcatcgctg tacttgcagt cctgaagtcc aagatacgtc agctgcggaa acagcttgcc
     4381 agacagaatt ggcgcgagat ccttgaccgt tccatcgccg ccatagtcat cgacaccaag
     4441 ccacaagcgg agggtctgca gcgacggcag ctctgccgaa aacagatcct tgagggtctt
     4501 tttggacagg cctccggtct ggagcagaat cgaccgcaac gtggagtttt tcagcttgga
     4561 aaagcgcagg ttctcaccgc cgcgaatcac caagtcttca agctgcggaa gcgcgcttaa
     4621 cagcggtcca tagtcacact gatgaatcca tgaaacttcg ctttcctctt gcacgatgtc
     4681 gccgacaaac agacctttga gcgacgtgag cttggcggca ttcgccagca gcttttcgca
     4741 catcgcgttt ggtcctttgt cgcagtaatc accgtaccac tgaccgatca ccagacctcg
     4801 cagctcgccg agttttttgt ctgcaagcag cgcatccagc cgtgcggtga aatcggcggc
     4861 gtcatcatcg tagctaatcc caacgcgaaa cacccgcttg tcgagatcgc gcagcgcacc
     4921 gtagctgcgt cctccttcgt catcaccatc gccgccatcc ccgacgacaa agttggtgac
     4981 tttgtagttt aggtagcgtg ccgcatggtc catgatgtcg atttcgaccg gcggacggaa
     5041 cgtgtctttg ggggcgctgc caccttgctc gatgtagcct ttgcgggtct tttccgcgat
     5101 cagcttgtcg gcttccgttt tcgcggcctc tggacttgtg caggccgtgg ttttgctggt
     5161 tcctgcggtg ccgatgcgtc catacgtcac cgtgaaggaa gaaccctcga tggaaatctg
     5221 ccagaacttg ctggaggcgc cttccaccat ttcgaaacga cgggtgtggt ttgtcatcgg
     5281 caggagtttc gcaggagtcc ggattctgtc aaactcccag ccttgtttgg tccgcagtcc
     5341 ccgattgcgt gcgatccaga atcgcaccag acccaacttt ccagtgcaga ttgttcggta
     5401 tttccgaaca gagtatccac aatcggcagg attgttttcc cgaggatcac aatcgtactc
     5461 tgattgccat ggcgtctttg caaaagctct cttggcgggc gattcagggt gtggctggcg
     5521 tgctgggatg tatgtacttg gtttctgggt acatcttcat caccacgcgg agcatcgagt
     5581 ttgtccacta taccggcgtt tctgcgcagg aagtctggac aatccgcaac ttgggagtgc
     5641 gggtgctggc gataggaatc ggactgcttt tggcgcttct gatgcgacga aaagacgcac
     5701 ttgcgttgat gctgcttgtg cgactttgtg ccgatgtggg ggactttaca aactcggcgc
     5761 tgacgccggg tctcgatgca tcggtggcgc gcacgcttgc ggtgttctgc gtcgttgaac
     5821 tggtggcgct ttcgggactt gtgcggattc tgtctgcgga acggaaaaac gctgcggcta
     5881 ccgatgggaa agcggtttga tcatgaatag gaaaatcccc gcttgggtga gcgtgcttgt
     5941 ctccatcacg gttgcgattc tgcttggcaa gtcgatcgcg attttccggg ggccgcttct
     6001 gccgcgggag attggcctac agctcgtaga gcttgcggga cgcaacgcag cgatgcttgt
     6061 tgcgtctctg cttgcgctgg tcacgcagcg tcgcgagtat ttcgttttgc tctgtgtgat
     6121 gggagtctgt cgcgagtctt gggacttcgg aaacgtcgtc tactttgatg gttttcggcc
     6181 tggttctctc ggacttctgg catttgtggg agtgtggctg gctgcgctgt ggacgctgcg
     6241 gaatcgtccg gtcggtgcct aaatcggcgt tacagactgc ctgaagttcg atcttgaatc
     6301 agtctccgta atgatcgagc cggatgattc ctttgcgggt gcagaccacg cgagtccgtg
     6361 acagcgtgcg cttgccactg acggtatcca gcgacgccaa aagcacgttc aggtgataga
     6421 cctgcggaat ctgcgccgac cggaaacgcc cggtttggta gtcgtaaaaa cgcacctgcg
     6481 ccaccggatc atcaagccgc gcgagcacat ccgcgagtga gagacgcaac agatcctgca
     6541 ccgcaaagcc cgcgggtgtc ggcgaacttc cggctcccag cgtaagctcg cgatggtagt
     6601 gcaggatttc ttcggtttcc gtcgtcacgc ctcgcacggt tcggttcagt acacgcagcg
     6661 ccatcacgtc ctgtggcacc tgcgattccg gcagaaaatg aacccgctcg cgcgcgcgtc
     6721 cggcgatgct gccaaagccc acttcttcca gcgtgtcgaa gatcagcttg cgatcgtgat
     6781 cccagcgcag aatttttgat gacagactct ggcgcgtcca ttccttgatg cggtctttga
     6841 gcacatacgc gacgaccgca aacgtcagca gcagtccttg atgatcgcgc gccaagtcat
     6901 agttgaggag tggcacctgt gccagcgtcg cccacgtcgc tgcaagtccg gcggcgacca
     6961 ttgctgcgct gttgcgataa aacggatcgc ggtccgcttg tcgggtatca atgtagagcg
     7021 cgcgctcgag gatgcttttg agccgaccaa ttcgatacga gtagtattcc ggcgcgtccc
     7081 cccacggggt cgcgaatccc tgatcgagcc gatagcgatt cacctgttcg atcgttcgcc
     7141 caagccgcac gcgcatccgc gttgccgttg acgttccatc gcgcagctcg ggaagtccgt
     7201 cgataagctc ggccagttct gccagtttca gatcgatgac ggcgctgacg tactcttcag
     7261 caaacgccag cgcttgcggc acttctttgg gctgaagcgg tgcaaacgcc gccaccttgg
     7321 cgcgcagccg atgcatgctg ccgagcgctt tcagcatgtg gcttgtcgct cgctccaggt
     7381 tttcgacgag ccgtttgcga tcgatgggcg acgctttggc ttggcttatg agctgccgca
     7441 ggacttcgac ttccgtggat gcggcatcgg cgacttcggc gctgtaaagc tgggccagct
     7501 tgaccagtgc acttgcccgc ggcgcatcgt cggtcaacag cagcggtagc tgttggcgca
     7561 gcattgtccc cggattgcgc ggactgttta ggtcggccaa atcggcgagc aacaccgtcg
     7621 gggaatgcag tcgcattcgg atcgacgcat cgccgtaaaa atcgctttgc gaatagcttg
     7681 tgtcgcttat cccaaacgac ggcggaaaaa agaagtacgc atccagcttg taggtccgtt
     7741 ccgggccgct cggcagtgcc agaagctgcg tgatttccag cgtgcgcggg tccggcgcga
     7801 caatctgcgg cggctgcccg agcccttcgc tcggacgacg caccggctga cggcgaatca
     7861 gccgcgatgc cagaagctga cttacgcgcg gggcagccac aggttcgtct ccagagggct
     7921 ggtttcgatt tcgccaccga gcggatcgat gagcggtctg acgaccgaga cgacgtggct
     7981 gggcacgccg cagtcgatgt gaagacggat gcgcgtggct tcctgcgaca ctccgacgga
     8041 aagctgttca cccagagacg gcgtggcttc gaccacagcg gacaggattt cggaaatcgt
     8101 gctggccagt cgaccttccg cgtcggtgac catcagctgc ggactgattc caccgattcc
     8161 ttcccacgtc acttccaggc gatgaccgtt ttccaaaaga ccgctgcgcg cttccttttc
     8221 cgcttggctt agcacgtcgg acaaaagaca cggcacgatg tcttccaccg ccggcaaaag
     8281 cgacgcttca atcgccggtt gcgcgacgcc aataaacacc cgcagcggcg ccatgcctgg
     8341 tcgcttcaga acgtgcagca gtccttggat gagctgtcct tcgataagct ccagcgaaac
     8401 cggctcggcc tgcccgtcgt tttccgccag tttcaggatg ctctgcacct gcggatgctc
     8461 ggcaaactcg ttcattcgcg cgccggcctc gggaccgctc agctgaagcg cccgtcgccc
     8521 cgcgccgttg caagcgatca tctgaccgtt ttcttcaaag attacgcacg cgcagggcag
     8581 ggcatcgaca agctgagaca aaagcgcttc gctcattccc gccgacagaa gccgcctccg
     8641 cagcgccgtc gccatttggt ttagcccccg cgccacatcg ccgacttcat cgttcgtcgg
     8701 ctgcagcact ttgaccccca ggtcgcccga cgccaggctc tgcgccatct gcgacacccg
     8761 ctggagcggt cgcacaaaca aaatcgccga aatcagggaa aacacgatgg ccaccgacgt
     8821 cgctgccgcc atggcgttga gcagaaatcg catcgtcccg ctcgtcaccg cttcgatgct
     8881 gtcgaccggc atcgccagcc gcagcacgcc atacggttga tttttcgcat cgacgagctg
     8941 cctcgcgaca tacagcgtct cgattccagt cgtgtccgag tgacggcgcg cgtaacccag
     9001 ccccaaaagt ccgctcagca cgggcagtcc ttcgggcttg cttgtggtgt cgcccatcgc
     9061 catccggact tcgggacggc ttgcatgatt tatcgaaagg ggcctgacca ccgacgtatc
     9121 caaaagcacg tcaccggcca aagacatgta ggtcagccgt ggcgtgctca tttcgccaaa
     9181 cttggtgatg agcggcagtc gatcggcttc tggcacgctg cgcaatagct cggcgacttt
     9241 ttggctttgc gtcgccatcc gcagcgccga ttcgcgcagc aggtcccctt gtagcaccac
     9301 gcggatgtaa aaaaacgtcg gtcccgcccc gagcaggagc accccaaggt acgagatcag
     9361 cagcttaact gggagaggta tgcgaagcat ggcaaccaaa gcgggtatct gacaccaaaa
     9421 cgccgccatg cacagcgagt tttttcttgc gtgtcaaagc cagtgcacgg taattctttg
     9481 ccttccggtc gtcacagacg acctttgcgc ttgtagctca gctggataga gcaacggact
     9541 tcgaatccgt gggtcgggcg ttcgaatcgc tccaggcgca cccaagttgt ccgaaggggg
     9601 gcatacgtag gcggccaact tgaccgtttg ttgacgaagc gaccgtcgcc gtgagcatca
     9661 aatggtcatg tttgaagggt tcgcaaacgt atggacgccg gtgacactgg cgagtcggct
     9721 tggaaagacg ccgcttgccg tcgagttggc gggggaaaag ctggtgttct tccgcgggcg
     9781 cgacggcaaa gtcggggcgc tggtcgatcg ctgtccgcac cgtggcgtca gactgtcgct
     9841 gggcagtgtg gcgccgtcgg gctgcttgac ctgtccgttt cacggctggg agtttgatgt
     9901 cgacggcaac gcgcggtttg tgccgctcaa tcccgatgcc aagctcgatc gcttaagcgc
     9961 gacggccctg ccggcccgcg agctgggcgg actgctgtgg gtgtataccg cgccgaagtc
    10021 gaccccggcg gaaccgatcg tcccagcgac gctgaccgat ccgaagctgg cgcgcaccta
    10081 tcttgaagag gagtggaatg cgcactggac gcgggcgatg gaaaacatgc tcgattcgcc
    10141 gcatgtgccg tttgtccatc gcaacaccat cggtcgattt gtgcgaccac gcttacagcc
    10201 caattccagg atggatgtcg agtgggaaga caccgatttc ggcggtcgca ccttcgccat
    10261 catcgacaac gatcgcagca tcgatccggg caggctcgag ttctatcgtc cgaacatcat
    10321 ggtgctgcac attccgatgc ccaagcagac gttccgaatg cacgcgatct gtgtgccggt
    10381 ggcggcacag aaagttcgga tgatcgtcgt cggagcgcgc agctttgcga agctgtcctt
    10441 gctcaatccg ctgttcaact acagcaacaa gcgcatcgtc gccgaagacc gcgccgtcgt
    10501 cgaatcatcc gatccgcccg tggtgccacc agccgctcaa gaagtgtcgg tgcgcagcga
    10561 tcgggccacg ctcgccttcc gcaagtatta cttccagtcg ctacacacct cgtcggtgtc
    10621 tgtcaaacgc ggcttgccgg ttgtgaccga ttcgccggtc tgattttcgc ttcgttttca
    10681 cattttgggg ggtcgtcgat ccgtggcttg gcgcaggggg cgcagcaacg gaaaactggg
    10741 cttgttgggc gcgctttgtc cgtgactgga ttttgccgcg ctcgggcggg cggctttgca
    10801 tgcggatttg cttgcttttt tgcccatggt ggatgactac ttgagatgct acctgttatc
    10861 tgtaatacgg aaaaatctag ccatgcatcg aagaacaccg ctctggcttc ctcttttcgg
    10921 cttgctggct gcaccggcgc tcggttttgc tgacaccgca gcgccgccag tttcggcttc
    10981 tcacgggcgc gcggtcgcgt cgttttgggg catccgccaa gactgctttt tcgacggcat
    11041 gatcgcagac gcagtcgcca gtcgggcggt gatcggatca ccgattcggg cgctcagcgt
    11101 gccgcgcaat gccgcgacga tgggctgttt tggcaaagcc tgcgtcgaag aaatccgcaa
    11161 agagtcggcc tgcgcagacc tcgatctgtc aggcggagtc attggtggcg aggccgccga
    11221 gtttgtcgag caaggccaac gggtctacaa gctgcgcttg tggaaatacc tgcccgcgac
    11281 ggactcggtg ctagagcacg aggcgctctg tcgatcggat ggtttttcaa gccgggctga
    11341 cttggcgcgc tgtgcggcgg aaaaagtggt tgagctggct tcggaacact tcaaagcgcc
    11401 ggtgcatctg gtcgacacgc ccgagtgcaa gccgctttcg gtgacgcctg cgccggttgt
    11461 cccatcgccg gtcgcggcag ccgaaaaggt gcctgtctac tacgccgtca cctacagctc
    11521 gaaaaatgaa atggggacgg ccaagagcgc acgggaacac ctggaaaagc gcttttcgtc
    11581 ggaacacaac ctggtgcgtc gttcgagcaa agccaacgca ttgagcgccc agggatcggc
    11641 accatcggtg gaaactgcgg cagaatatct gcgagccgtt cctgcgacgg aaagtgcggc
    11701 ggcaccgctt ctgcttttga cccatgtgca tgcgaaaaac agcacttgtg ggacgtggtt
    11761 tcgcagagct cccgacggaa acatccagaa gtttggcgaa acctgcggtc cgatcgaagt
    11821 gctcgacgcg ctgccgcttc ccgacagcaa cattgagtcg atcccggtcg tcccggcgga
    11881 acccaaggtc gcgacgcgcg ccaagacgcc cgacttttgc cgcgcctcga ttccgcagtg
    11941 tccgtcgtcg ttcccgtttg cgaccgtgct gccgaaacac ggcggatcgg tgggcaagag
    12001 cctgctttca cgcggtccgg gctggattcc gatcatcggt gctggcgccg gtctggtgac
    12061 gatgattgcg ctcggggttg aagatcgaaa ctatcggctg tcgtggccgg aaaatccggg
    12121 ctggcagtcg ccggaacacc tgctggcttc ggcattttgg accgcgacgg gactgacgat
    12181 tgcgctcggc gcgagtgcgg cggcagtcgt cgtcgatcac gtacagcaca aacattccgg
    12241 ttcagccgcg agcccacagg cctgctcgtt tgcgtctgag gtgcagccat gaaaagcaaa
    12301 cccacgctcc ttcgactttg cgggctgctt gcgctatcgc ttgccccagc gtgcattccg
    12361 tccggcgcga cgctgccgag tggcatcctg acggctgatg gtggcagcga cgtgcccgac
    12421 atggcgatgc cgcccgtcgt ccgcaagtgc aaagacttcg atgcattcgc gggcagcgag
    12481 ctaacctgct gggattttac cgacgacgca acgcgggctt cggtaaatga ctgggcggcg
    12541 ctgcaagacg tcgtcaccaa gtcatgctgg tctgcatcga gcacgactcc gacggtgctg
    12601 caaggtggca atgccagcct gccggccaaa gtcgagtgca gcctcgcgct caccggcaat
    12661 tccagcatca gcggcaaagg actcgacctg ggcggtgttt cgagcgtgac cctggcgatg
    12721 agcttccagg ttgccggttt tgcgaatccg ccgacgcaga gcgcattttt cgtgcggcct
    12781 ggtctgctgc cggaaattcc gcttggcaca atcggtgggg cgcccaaaga ctttcagtca
    12841 tcggccctca gcttcccggt gtccatcgac cagagcgcca actttcagtc gagtgacctg
    12901 aagcttacgt ttgcgcggtc gcttctggac cagcagatat tcattcgttc gattgccgtg
    12961 ctgggaacgc ctcgtcgcta gcggtcgatt ccgtcgctgg ccgttctgtc acttgccccg
    13021 tattgccgcc ggcggatacc cggagggacg atgaagctga ctccatttgc gaagctgttt
    13081 atcgcgttgg taatcgtcgc agcgacggcg tatgtcgtca aagtgaacgt ctacgatccg
    13141 gcgcggcagg ccgtcgagct tgcggcccag cgtcacacca aaggcggcga tgtcgagcca
    13201 gcaaagcccg tcgaaaccgc agcggcaagt gcgcagacag ccggcccaga tcgaccgatc
    13261 gtcattggca tcaatgactt tggcggcggc tatccgctgc tgctggccaa cgacggggcc
    13321 aagccgggtc cgcagtcgct gtttcgtcag gccgggctgg atgtcgaagt gcggctgatc
    13381 cgcggatcga aagagcggct caaagcattt gacgacggcg aagtcgacgt gatgctgctt
    13441 acgctcgact actttgcgaa ccttgctccg ctttacaaac agaaaggcgt cgagctgcgc
    13501 agcttcctgt ttgtcgattg gagccgcggc aacatcggca tcgcggccaa gccgcagtgg
    13561 accagcatcg aagcgctgcg gacggcacgc attgcgacga cgcgcaacac cccgacccac
    13621 tattttctgc tgtcgctgct ggagaagagc aatctaaagc ctgccgagat cgacaccatc
    13681 aagagcagcc tggtgtttgc gaccaaaacg cccgatgcgg gggcgctgtt tcagcgtggc
    13741 gaagtcgatg cggtggcgat ttgggaaccg cacctgtcgg aagcgattgc tggcggcaaa
    13801 ggctcgacgc tggtgacgac cgaaaccgcg accaacttgg tcggcgatgt gctgtttgcg
    13861 cgcaaagact ggctggaaaa aaaccgcgac aaagtggtgg cgctgtcgaa agcctatttt
    13921 tccgccgtcg atgcgatgaa caaagatccg cagcacgcga tcgagctttc ggcggcggcg
    13981 ttcgcgcaga aacccgagca gatcgccgcg aacgtcaaga aagtgaagcc ggcgcagttt
    14041 gctgacaacc gggcattttt tggcctggaa accgaggact gcgcgtatga ccagctcttc
    14101 gttgaagcga gcggtttttg gcagcgcgaa ggactcttca aagagccgac gccgcctgcg
    14161 cagagcaagt ggcttcgggc gctcgaagcg ctggcaccgc agtacaaaga gcaaaaagtc
    14221 gcggagtcgt ttcacttcca aagtctgccc aaagcggcgg ctccgtcgct gctcaccaag
    14281 agcgtcagca tctattttgc gagcggacaa gcgaccgtgg accaaaatgc gcgcaaggtg
    14341 ctcgatggat ttgcgcagac gctggcggtg tttcaaaacg cctatgtgca ggtcgaaggc
    14401 aacaccgaca acgtcggcgc gcgtccatcg aacgtggcgc tgtcgaaaca gcgggccgaa
    14461 tcagtggtcg cgtatctggt cgatcggcat cgcctggacc gcagccgttt catcgccatc
    14521 ggcaatggac ccgatgcacc cgtcgctgac aacaaaaccc ccgaaggccg cgagctgaac
    14581 cgccgcaccg acttcaagat catcaaaaac gcgacgccgt agcgcttacg ttccgttttg
    14641 caaaagcagc gcgagcacgt tttcgacccc tttgtccccg acgcgcacag tcagatcggg
    14701 atgttcgggg acttcgtagc ttgtgccgct gccggggagc gtgctcggcg cttgtttgta
    14761 cagacctttg ggatcgcgac gctggcattc ggctagcggt gtgtcgacga agacttcgac
    14821 aaagtgcggg gccagactgc gggcggtatc gcggtatttt tggcgatttg ccgtcgctgg
    14881 aaccagcacg ataagacctt gtttggccag cagcgccgca agccccgcaa gcgaggcgta
    14941 aaagtctgtg cgcgcctggt cgtcgtagcc gtagctgggc cggatcaccg cccggacatc
    15001 atcgccgtcg agcacgcacg cggcacggcc ttgttccatc agcttttggt gtacagcgcg
    15061 ggccagcgtc gattttcccg acgcgggata gcccgtcagc cacaccacac agccgttttc
    15121 gctcactgca tcgcctcgtg gccccaagcg gggtcaaact gcgccgcatc gagcacgcga
    15181 aagacaaacg ccaaaatgcg acgacggtcg gcggcggcca ggtttggata ccacagcgga
    15241 ctcgcgagca cgagaccacg ccaagcaaaa tagggcgcca cggcgtgcag tacattcggg
    15301 gcggcaatct gctcactttc ggcgagatag acctgccaga agcgatccca caagcgaccg
    15361 agtccgtgtt cccagatccg ccgatgttcc agtccgaaaa acagatagtt tatcgccaga
    15421 caggccacgt cgtcggcggg gtcgccttcg gaaccgcggc ttgtatccag caagtggagc
    15481 ttcccggcgt cgtcaaacag gatgttgaag ggatgaaagt cgccgtgggt tttgcgcagc
    15541 ctgtccgtct gaccgcgcag cttccagcgc cagcttaggc acttttcttc gatgcggcgc
    15601 agctcgtcca actcgatgcc cggcgtcgtg gccggatagg aatcgaccaa accgaaaatg
    15661 gcttcgccat gcccgagcag atcgcgaatc gcgcgggtgt acgccgctgg atgcgaaccc
    15721 ggtgcggcgt gaatttcgat aagcgcccgc gcgagcactt cggcccgctc aagatccgtc
    15781 ggtgtcgcat gacctgtcat cgccacccgg cgcaggtcat cggcgtaggg gttgccatcg
    15841 caccactcgg tgacgacgta aaactcgccg gcgtcgcgca gggaaatcag gtcgccactt
    15901 tggcgcagcg ctcccacatc gacggcacgg gtatgaaagg gaatccgatc cgcactgtcc
    15961 cacaagagca acatggcctg cgcgcgatcg gatcgacggt catggccaaa gtcatcggga
    16021 cgggcagtgt gaaacacaag tcgccgcgga ccatcggggc cctccagatc gacgcgcacc
    16081 gggcgaccgt agcccagacc cttggtggtt tcgtcgctgg catcgtcagt accaagcgcg
    16141 gaaatgcgct tgatccgcga ttcgggatac agcgaaagaa cgtgtctggc cagggcggtg
    16201 ggggacgaag acgacggact catggcggca gaatgccaca acttgcagtt tccgttagcg
    16261 ctcgcgctga ttgtcttcgc tgtcgctgac ttcttcgtcg aggatttcgc tgtcttcggc
    16321 gtcgccaccg ccgtcttcac catctgggcg ctgcacaggt cgcggcgcaa tcacgccgtc
    16381 ttcgcgtggc agtggggccg acggaaccga cttggcggcg gcagcataga caaaagacac
    16441 ttttttggcc ggaacttcga ccactttgtc gccccattcc atggccacac cgacgagcgc
    16501 ggcgcgtcga tacaaaagcg gcaccgaaaa cgtgcagcgc cagcgcaagt tggctggaac
    16561 gagcgccgga tcggatccat cggcaggcag ggacactccg acgggcggct gacagtcggc
    16621 catgtcgggc acaaaacgcc gtccgcgatc atcggtcagc acgagcaagc ggatttccgg
    16681 aacgtgcttg gtccccacga cgatgagctg gccttggccg gtcaccaaat caaactgggt
    16741 cagttccaga ctctgcggga tttccggcgg acggtagtgc ttctgcttct tctttttgcg
    16801 cttcgacgat gcatgcttgc gctggcctga tgaacttgcg tgcgacgacg acttgcgtgc
    16861 ccgcgcgagc gccagttctg gcgctgtcac aagcagccca agcgaaacgg aaaggagcaa
    16921 aagctggcgc agcatttttg cggttccccc agcggaaggc gtgactagtc gtagtactcg
    16981 gtgccaaggt gggtgataag ttcttcaccg cgcagatagc gaagcgtgtt cttcagcttc
    17041 atcagctgaa tgaacaggtc gtgttcggga tacagccccg gtgcggtcat cggcgtcttg
    17101 aaataaaacg acagccattc ctgcacgccg ctcatgccgg accgactcgc cagatcgagg
    17161 aacagcgcca ggtcgagcac aatcggcgcc gccaggatcg aatcacggca gaggaaatcg
    17221 accttgatct gcatcggata gccgagccag ccaaagatgt cgatgttgtc ccagccttcc
    17281 ttgttgtcgc cgcgcggtgg gtagtagttg atgcggacga cgtgatggaa gtctttgtaa
    17341 agctgcggat acaggtgcgg ctgaagaatg tattccagcg cgccgagctt cgacatttcc
    17401 ttggtcttga acgattccgg atcgtccagc acttcaccgt cgcggttgcc gaggatgttg
    17461 gtcgaatacc agccctgaag tccgagcatc cgcgctttga gcatcggcgc gatcacggtc
    17521 ttcatcagcg tctggccggt cttgaagtct ttgcccgcaa tcggcagacc ttgctgcttg
    17581 gccagctcgt gcatcgccgg aaaatcgagc gacaggttcg gtgcgccgtt ggcatacggg
    17641 acgccgcttt tgaggcacgc ataagcgtaa atcgccgacg gtgcaatcgc ttcatccgat
    17701 gactgcagac ctgcttcaaa cgccgccaac gatgaatgaa catgcgtcgg cgtttgatag
    17761 atttccgtcg atccgcacca caccgcaacg cagcgcgaca gatcgttttg tttcttaaag
    17821 ttcgcaatgt cagcgataag ctgctcggcg aggtcacgct tcgacgtgcc ctttttgatg
    17881 tgcgtaccgt gcagcttttt gacccagtgc ggctcgaaca cgccggccat cggacggacg
    17941 ctctgaaggt acggctgaat ctgcccgagc gtgtctttgt tgagtacgcc cgcgtgcgac
    18001 gcagcttcga atgcgtcgtc ggcaaagatg tcccagccac cgaacaccag gtcgtttagg
    18061 tccgcgagcg ggacaaagtc tttgattttc ggggtccggt tttcggtccg cttgccgatg
    18121 cggatggtgc caagctgcgt cagcgatccg atgggctcgg cgagtccgcg acgcacggct
    18181 tcgacgccgg caatggtggt ggtggaaacg gcgcccatgc ccggcataag cacgccgagc
    18241 tttccttggg ctggtttgat ctctgagccg cgtgaaatgg ccatgtgcaa gttactcctt
    18301 taggccctcg tgctcctgca tgttgcgagc cagccttccc aacgttgctt ggtcggcttt
    18361 gatgcgcagc ttcgccgact gttcgccgaa ctcttcgtgc agcacttccg tcgtcgcgaa
    18421 aatctgcgcc cgcagctgcg gctggtcgta gcgcacttcc agttccgttt cgatgagacc
    18481 ttcgtcgaag aaatcaatga tgagctgtcg cacgcgcgca acgtcatcgg gatttttcgc
    18541 cgagatcgac acctgacgcg gaaaacgcgc ggtccactcg tcgggaactt tgccgtcttc
    18601 gagccgatcg gtcttgttaa acacgatcag ctgcggaact tcggctgcac caatttcggc
    18661 cagcactccg caggtggtat cgacatgacg cagcgccgcc gggtctgaag catcgacgac
    18721 atgcaggatc aagccggctt ctagtgcctc gtcgagggtg gtcttaaacg aggccaccag
    18781 cttggtgggt agattgcgaa tgaacccgac ggtatcggac ataagaatcg tccgtcgtgg
    18841 ttcgggatga agttgtctga ccgtggtgtc cagcgtcgca aacagcttgt cggcgacata
    18901 gacttccgcc ccagtcagcg atcgaaacag cgtcgatttg ccggcattgg tatagccgca
    18961 cagcgccacg cgacgcagcc cttggcgacg ggcactgcgc gttttgtgct cccgttccag
    19021 cgcgaccagt tcatcgcgca gctcggcgac gcgatcgcgc agcttgcgtc gatccagctc
    19081 cagctgcgac tcgccggcgc ctttgccacc gatgccacca cgcacgcgat cgcccattcc
    19141 ctgcgtctcg cgcagccgcg gcgccatgta ttccagacgc accagctcga cctgcgcccg
    19201 cgcctgccgg ctcttggcgt gacggtgaaa aatttcgagg atgaccgcag ttcgatcgag
    19261 caccaggtcc acacccgtcg ctttttgcac attgcgcgct tgcgacggtg aaacctcgtg
    19321 gtcgattagg atcgcggtcg gcacttcttc gcgtttgcaa agctgcgcca gatcctcgag
    19381 cttgcccggt cccaggtacg ccgccgaatc gaacgagccg cgtttttggg tgatttcacc
    19441 ttcgacctgc atgcccagcg ttgaggcgag ccgggtcaat tcaccgagcg attcccgaaa
    19501 gctcagctcg tcttcttcgg gaagttgaac ggcgcacagg atggctcgct tggggagtag
    19561 ttgcaaaaga tcgggaacgt cagacgagga attgctcatg taagtcgtgt atctcggcgc
    19621 ggcaaggaag gcggcacctt agcagcgagc ctgccccctg tccaccggcc aagcgcgcgc
    19681 tcgggctggg tttttcgcct gtcagtgcgt cgaaaattcg accatcgcgg acgtggattg
    19741 ttcaattcct gacagcggtg ttcaccttgc actgcgtggg cgaagctgcc gataatgggc
    19801 cgggattttt tcgccgtgac ttccgtcctg ataaccgatg ggcatgagct ggccggactc
    19861 ggagcggccc gctctctggg acgggcggga cattttgtgt ctgtcgcggt gccgcaagga
    19921 atcgatgctc ccgtcggaaa atcacgctat gtcgcgcggg tcattgcatc gcccaatccg
    19981 tgggccgatc agccggcgta ccgacagttt ctgcaaagcg aagcgcccaa gcacgacgtg
    20041 ctgttgccga tcagcgaggc ggcgatcttg gcgacggcgg cttgtcgtca taatctggga
    20101 cggacgctgc tgcttttgcc atccgatgcg tcgctgcgct acaccctgag caaataccac
    20161 gcgacccgtg ccgcgcaggc cgctgggctc gcggtgccgc cgacggtgtt cttgagtgat
    20221 ggaaactggc ctgcggacct tttgccgctc gtcgattcgc tggccggttt gggctatccg
    20281 ctgctcatca agcacgacaa ctgtcttttg ccgtcgggac agtatgtgcg gggcaagacc
    20341 gaacgggtgg attcgccgct gcaagcgcgg gcgcttctgg ccgagctgcg ggcgcttggc
    20401 gcgcaggtga ttgcgcagaa accgattcct ggtcatggcg caggtgtgtt tttgcttcgc
    20461 cacggccaaa aaacgctgct gcggtttgct catgagcggc tgcacgaagt gccctatacc
    20521 ggcggtgttt cgtcgctacg catgagctgt cacgatgaaa agctggtggc gcagtccgag
    20581 cgatttttgg ccgcgctcga ctaccaaggc gtggcgatgt gcgagtttcg caagacgccg
    20641 agcggcgagc cgctgtttct ggaaatcaac ggacggctgt ggggatcgct ggcgctggcg
    20701 ctccactgcg gggtcgattt tccgcggacg ctgcttctgg ccatgcaagg aatgccagtt
    20761 cccgcgcaag cggactatcc cgatgggctg cgctgtcgca acctgctgcc gggggaagtt
    20821 tcgcatcttg cgtcgctgtg gcgcgcaaaa gacgtgtcgc tttctgacaa gctggtggct
    20881 gcggccaagc aagtcctgct gacgtttgat ccccagattc ggcatgacca tttctggttc
    20941 gatgatccgg aaccggcagc aaagcagacc cagaactggc tatcggagtt tgcgcagcga
    21001 ctgcgaggtc gcgtagagag gaacagacag atgcagagcg ataaggcgct actttccgaa
    21061 gaacttgcgc gcacggagtc gcgctttggt gcgcttccca cgccgcgccg gattctgacg
    21121 ctgtgcctgg ggaacatctg tcgcagtccc tttgccgagc actatctgcg tctttcggcg
    21181 atgcaaaaag ggctgccgat gctcgtcgtc gaaagtgccg gttttttgca ccatgatggc
    21241 cgcgcgacac ccacgcgatt tgtcgaactg gtgaagccgc acggcgtcga cttgagccat
    21301 caccgcgcgc agcgggtgca gcgggaacag attgatgctg ccgacctgat cttggtgatg
    21361 gatgtgcaga acctgcgtgc ctttcatgac gagtttcccg atgctgtcgg caagacgttt
    21421 ttgatcggag cgctcgccaa atcgcacagt ccggaaattc ccgatccata tcttttgccg
    21481 gtgcgcgaag ccgaagccag ctaccgcttg ctcgccgagt cgtgtgccgc agtgatcacg
    21541 cgcattgtcc acaaatcgac actgcgccgg ctgtgatacg ctgtcttggg aatgaacatc
    21601 cgtcggcttt gtggatggtg tgcgctggtt ccgcttgggc ttctgctggg ctgtggtttc
    21661 acgcggcagc acgcggccaa gtcacaggtt gatgtcgtcg aagaccagcc ttatgtcagc
    21721 gcgagtccgc atccaaaaca ccggctcgat ctgtatctgc ccaaagtgcg acacaacgtc
    21781 cccttggtgc tgtttgtgca cgggggtttt tggaaaaacc aagataggcg ctactaccag
    21841 ccgtttaccg gactgcatgg caatgtcgga atggcgctcg cgcagcacgg atttgccgtc
    21901 ggtgtgacca gttatcgact gtttccttcg gcaaacatcg acatgcagct tgccgatgtc
    21961 agcgctgccg cacagtggat ggttgatcat gcaaaagact gggacggacg cagtgatgcg
    22021 ctgtttctgg tcggattttc tgcgggtgga cacttggtga tgtcgctgtg cgcggaccca
    22081 gcgcggcttt cggcatcggg aattcccgtc gctgcgctcc gtggctgtgc gtctttgtcg
    22141 ggcgtgctcg acattcctgc gctggcggcg caaaaagacg atgcgttcaa tcgcgagctg
    22201 acctatccgc tgtttggcaa cacgacagaa tcgcaggccg catactcggt atttcgcacg
    22261 atttcaccgt cgctaccgcc gatttttttg ctgtgggcgg aaaaggacct cccgtttgtg
    22321 aaaacggcgt ctcttcacgc gctgcagaag cttcagcagg cacacaaaga ggtcgattcc
    22381 ttcgagcttt ctggctacga ccactccgac gtggtgctgc ggattttgac cgatcgcgac
    22441 gatctgaccc cgaagcttgt gcgctttttg tccgcgcaca gcgggccgta acattcgtcg
    22501 gtccggtcac ttctgtctgt gacggttagc ccggcccctg cacgcacgac gcgatgttga
    22561 aaaacaggta ttcgagcagt cgttcctgtg gcgaaaaatc gactttgcag gcgcttggcc
    22621 agcgcgccgt ttcgtcgacc actccgtccg gttcggtgtt gtaggtcgaa aagtgaacgc
    22681 gcccgcatgc gttgtaatcg aacgtcacag tctggggacg attgccaaaa cccgacgccg
    22741 gaccatcgac ccagactttg gtcttggtct ttggatcatc gctcgctttg gaaaccacca
    22801 cgaagctgta atcgacggcg acctggctgt tgcggtttat gacaccgggc atttgggaca
    22861 gccacttttc catgtctttg tcgagcacgc ggtactgccc gccgtagtag ctgcgggagt
    22921 ccgcggccat tgccttttcc ggcgccaaaa gacacatcgc cggacccgga atcgactgcg
    22981 gttcaaagca cagatacggc gaaaactccg gcacctgttc gatcacatcg taggcccagt
    23041 ccgtcacata cagccgtccg cccgagcgca cgaacttttc caggtttttt ttcacctgcg
    23101 gtgccgccaa cagactctgg tactgattgt ccgaacagtt cacgatcacg atgttgtagg
    23161 aaagcagctt gttttcgtcg ctgagcagcg ttcccatttc ggcaatggac ggcggatttt
    23221 tcatccccac ggcgcgtccg ttgtacatgt ccagttcatc aattccgatc cgcttgagca
    23281 cgcactcaat ctgatcgtag tcgccgctta cgacggcaat tcttggaatg tagtcttgtg
    23341 gggacagctc gcggttcttt ttggaaaagc ggattccttt gcttccagcg tctgcgggca
    23401 ctgtattttc cacgcacgcg gtcgcttcca cagacaccac tcgctgaaat cggccaagcc
    23461 gcgcgacgac gggaaatgcc tttccggctg gcgtgccgcg caaacggaag ctgccatcaa
    23521 aatcggtgat cgccgaagtg acagatggtg gtgtgtcgtt gcacagatcg cagcccagac
    23581 cgggcggaaa ttcgggcagg ttgtcgacgg gaacaaatac cgatgcaccg ggaatcggat
    23641 cttttccgtt tcctgcatac agcttgccca ccacttcggt cgtcgtgccg ggtggacact
    23701 tgggaacgtc gcagaatcgg cctttgcaga cgggcggtgg gatctgcatg tcgccaccgg
    23761 gacggaatcc atcgctgcga tcggtcggac tgctgtcatc aagcggcggg cgatcgacgc
    23821 tgcaaccggc gctcaggaat accaaaacca gcgaccaagt tgcgaggatg ctgcgagtca
    23881 tgcgagcctc caggtgtctg caagcagagt ttgtacgcaa ggaagagtca acgtggttgg
    23941 gacgctatct gctagaattt ccgcggtcaa tcgtcgcgat ctgtttgcca cggttttcgc
    24001 gttttgtttc gtcgcggatg tcggttgtga aacgcggcgc ggaccggcag accacagcgc
    24061 agcgactggg gcaagcgagc tttcggcttc ccaacttgcg gcccaggatt cctttcggcg
    24121 tccagaccgg ctccttttgg cactgggaat tgcgcgcgga cagaccgtgg cagaaatcgg
    24181 cgcaggcggc ggctacctga cgcgccatct tgcgcaggca gtgggaccgt cgggaaaagt
    24241 ggtggcgacc gacattgaca aagcagcact ggccgcgctg cggtcgcgca tgcaagcagc
    24301 ttttcaagtc gaaacgcggc tggttccgcg cgatgcatcg ggtttggaac tgtcccgcta
    24361 tgacgtgatt gtgctggccg atgtcgtaca tcttctgccg cagccggatc gctatctgcc
    24421 agcgctgttt ccgtctttgc gaccaggcgg acatctgtat gtcgtcggac gtgaagatcg
    24481 catgcccgga cttgcacagg cagcgaagcg cgcgggtcta cagattcggc cagttcaggc
    24541 ggatctgccc gcgcagtttg tgatcgaact tcttttgccg taatcccctg gctctgtgcg
    24601 aagatcggac aaggagtgat ccgcttatgc gcgatgctgt cccacccaca gagtacgatg
    24661 ccgttcccta tccctgcgga ggccacgatt acacgcatcc cggccacctg gcgatgctcg
    24721 cgacactgtt tggtctgtcc gcccctccgg ttgcaacttg cagagtctta gaactcggct
    24781 gtggcgacgg aagcaacctg attccgatgg cgctggcgat gccgaacgcc acgtttgtcg
    24841 gaatcgatct gtctgcgcgt cacatcgaaa ccggacagca ggaagtcagt cagcttggga
    24901 tttccaatct gtctctgcgg catcaggatt tgctgacgtt tggcgacggt gaacagccgt
    24961 tcgattacat catcgggcac ggaatttatt cgtgggttcc ggaagcggtg cgcagcaaga
    25021 tttgggacat ctgtagcaat ctgctgtcgc cccatggcgt cgcgtatcta agctacaaca
    25081 cctatccggg ctggcatcag cgcaagatgg tgcgcgacat gatgcgcttt catacccaac
    25141 gactcgacga tcccgataga agacgggcgc aagcgctggc gatggtcaac ttcattgcgg
    25201 gggcggttcc ttcgcagttt ccgctgcacc gcaaagcact ggaaacagag cttgcacggc
    25261 ttacggagct gccacggaat tacatccttc acgacgactt gggcgaaatc aatcagccct
    25321 attactttca cgaattcatc accgcagcga cggcacacgg actgcaattc ctatcggaaa
    25381 cgcggttcca tgtgatgcag gacaatcggc tgtctcccga agcgcgcaat accctgcggc
    25441 aagtgggtga tctgctggtg atggagcagt atcgcgacta catcacttgc tctgcgtttc
    25501 gtgaaacgct gatctgtcac gccacgcgat ctctggaaag acgcattcaa ccgtcacaga
    25561 tggagcgttt ttcgtttcaa tccagcctgc gcagccagtc ggcacagccc gatgtggtgc
    25621 atgcggtgga tgaaaaattc cagtcggacg gaatgacgct ttccctgtct gaaccgattg
    25681 ccaaagccgc gcttgtgatc atgcaacaga atgcgcccca agcgattccg ttcgagacgc
    25741 tgtttgctga ttccttgcgg ctgtgtcgcg ccgattccca agccgatttc gatctgcgct
    25801 tccggatgaa ccagcggcaa gagctgagcg agcttttgct ggccggcttt ggtgcgggcg
    25861 tgattcgccc gacgctgttt caggctcctt tctgtctgcg tccgggcgac aaaccacagc
    25921 ttgaaccgct ggcgcggctg cgcatgtcgg caaacaagga agtcaccacg ccgctgctcg
    25981 acaacatccg ttttgatgaa ccgtttggcc gggcggtcgc gctgctctgt acgggacagc
    26041 gcagccgttc cgacatggtg acagagcttt cgcagcgaat cgccgcgggc gagtttcctt
    26101 ccgagctttc gggcggcagt gttcccagtc ccgaagcgct gcgtaccatg gtcgaaagcc
    26161 ggctcaccga ggtcttggac cgcatcgccc gctacggaat cctggtcgaa aactaatttg
    26221 ctgtcgcttt tgttttcgcg ggcgttatgg tccgcagatg gacagacgac tgggtgtttt
    26281 cgcttctgtg ctggtcgcgt ttgctacgct ttccctttcc gcatgtgatg gcccccaagt
    26341 ggacccgcca ccgattgata ccagcgtcgg tagcggacct tcccgatacg tccaggtgtt
    26401 tgtcggttcc gatgatgcag agggagtcgc agatcctgtc acaaatgggg cagggggatc
    26461 gacgtttcca gcggctgcgg ctccgtttgg gatggtgcag tttgggcccg atacaccgtg
    26521 ggcgattccc cctggataca agtacagcga cgatcagatc gtcggttttt cggtcaatca
    26581 cctaaacggc gccggctgtc ctgcgcagcg cgattttccc ctgacaccga tggtgggcga
    26641 tgtcgatccg gaagcgacgc tgccgttttc ccacagccag gaagttgctt ctccggggct
    26701 ttatgaagtc cgtctcgcat cgggtgtgac cgtcgatttg accgcaacgc cgcgaaccgg
    26761 aatggcgcgt ttccgttttc ccaaaacgtc cgctgcgcat ctgcttttgc gcagtgcgat
    26821 gccgtgggat ctgctggtcg caaaggatgc cagtctgcaa gtggtttcat cgacgctggt
    26881 gacgggctgg cgaagcgatg cattttgcgc gatgggggtc ccgaccaaaa tctattttgc
    26941 ggcgcgattt gatcgtccat tttccgcgcg ttcgtttggg accaaagatg aaacccaaac
    27001 ccacgcagag ggaattcgat ccggtcttgt gctgtcgttt tccagcgagc aaaaccccat
    27061 cgttcacgta aaaatcggtc tgtcatcggt cagcatcgac ggggcactgc gcaacctgga
    27121 tgccgaaaat ccagactggg attttgcggc gctgcggaaa aaaacactcg cgtcctggga
    27181 tgattatttg gggcggctcg cggtgagtgg gggaagtgaa gaactgctca cgatgtttta
    27241 ttcggcgctg tatcgcgtct tccttcagcc gtcggtgtgg agcgatgtcg atggctccta
    27301 tgtggggttt gaccaaaaca ttcatgcatc cgatggccac gttcactacg cgaacttttc
    27361 cggctgggac atataccggt cgtggattca gcttgtcgcg tggcttgcgc caaaggaaac
    27421 cagtgacatc gtaaggtcgc tgattctcga cggacagcag ggtggggcac tgcccaagtg
    27481 ggcctatggt gcaagtgaaa ctggcgtcat gatcggcgat ccggcggatg cgctgattgc
    27541 aaacgcttgg gcgtttggcg cgcaagactt cgatcccgcg gcggcactcg cgcttatgtt
    27601 gcatggcgca aaagatccca ccgcgacttg caatgaactg cgcgtccgtc cgggtctgtc
    27661 cgattgtttg gaccgccatt actgccctgt cgatggtcca gacggagtct ctggttccgt
    27721 cgctgcgacg cttgagtatg cgattgcgga tgcggcgctt ggtcagtttg cggcgatgca
    27781 aggcgatgcc aaaaccgcgc gtgagtttgc cgatcgatcg cgctactggc gaaacgtatt
    27841 tgatccgcgg gccaaagccg gcagtgcggt cggacttctg caacctcgac ggcgcgccga
    27901 tgtcgcgggg gctgcggact ttgtggtggc caagtatgac gatggcaaag gagtcgtcga
    27961 agggagtctg gagcagtaca gcttcatggt gccgcaggat gtgcctggtc tgatcgatgc
    28021 gatgggaggc gatgcggcct ttgtcgcgcg gctcgatgaa cacttgcaaa aggtgaatgc
    28081 cgggaccaag agcccgcatt tttacatcgg caacgaaccc ggttttgcga cgccatggct
    28141 gtatccgttt gcgggttcgc cgtggaaaac ccaagaagcg gtgcatcgca ttgtgacccg
    28201 cagtttttct gccactccgc gcggtcttcc cggcaacgaa gacctgggcg cgctgtcatc
    28261 gtggcttgtg tttgcgatgc tcggaatcta tcccgaagcc cccggagtgc ccggttttgt
    28321 cgtcggcagt ccgtttttcc cgcagatcgc cgtgaagcgg cccggacaag cggacttcca
    28381 catcagcgca ccgggtttgt cggaccccgt ctatttcatc gtggacgcga cgctccaggg
    28441 cagaccgctg cgggttccgt ggctcacgct cgatcaaatg aaagccgggg gcgagcttac
    28501 gctgaccgta tccagccgtc ccaacctgtc gtggggcgcg gcacctgccg atcggcccca
    28561 aacccagctt ccttaggagt ccctgcggtg gaaagttctt cgcactatct tttgtgccgg
    28621 ggccgcgagc tgcactacct ggactttggg ccgcaggatg cgcccgtggt ggtggcgtgg
    28681 catgggctcg cgcggaatgc gcacgacatg gatgttgtgg cgcagcatct ggctgggcgt
    28741 tttcgggtga tctgtccgga caccatcggg cgtggtctgt ccgcatggag ccccgatcct
    28801 gcgcgcgagt actgtctgga cttttacgtc gagcttgccg agtcactgct ggatacgctc
    28861 ggcatgtcgc gctgcctgtg gctgggcacg tcgatgggcg gcgcaatcgg tctgcgcgcc
    28921 gcggcgggcc ggctgcgtgg tcgaatcgat cggctgattc tcaatgacat cgggccagag
    28981 atttcggcag cggcagtcga gcggattcgc agctatgccg gccagccgcc cagctttgcc
    29041 actgtgcgcg agcttgcgca gtattttcgg accatctatg cgccgtttgg cacgctgtcc
    29101 gatgcgcagt ggctgcactt gaccgaaacc agcttgcgcc gcttgcccga cggacgggtg
    29161 acgccccatt acgatccgca gatcgtgcag cagttcatcg tccatccgca cgactatgcg
    29221 cagtggccgg cttgggattc gctgtcgctt ccggtgctgt gtctgcgcgg cgagcgctcg
    29281 gatctgctct tgcccgaagt caccgagcag atgaaacggc ggggtccgcg cgccgacgtg
    29341 atcaccatcc ctggctgtgg tcacgccccc gcgctgcacg ttcccgaaca gctcgccctc
    29401 atcgacgagt tcttttccgc gaaactggtg aggtcggggg gcggttagtc ggtccggatt
    29461 tcggtggcgc gcagcgacga ttcggccagc cggtcgcggt gaaagtgcag gtccggcagg
    29521 cgcttcatct gcacggcatc ggccagtcgc acgcgcaaaa acggcgtcac tttgcccagc
    29581 gcttcgtgaa tggtgcgcag ctttgcttcg acggcaattc gcgcttcccc cggcgtcttt
    29641 ttcgctttta catctttggc ttcttccggc ggtttttgaa tcgcgaacat cactttcgcg
    29701 ttgcgcagat ccggactcaa ctggagcgac gttggcacca catcttgaag ccgcggatcg
    29761 ctcacttcga gtcggaacaa acgatacagt tcctcgaaca tcaggtgctg aagccgcacc
    29821 acccgcgacc cgagcgactc gccgctatcg agtgaaaatg gctcatcggc ttcactgcct
    29881 ttgcgccctc tgtgctgtct agacatgggc acgttgtagc gcgtcgcaca agcgattgcc
    29941 aagccgctac actaccgctg cgatgaagtt tcccgatgat gcgaatgtcg gcgccgaaaa
    30001 aaaagcgctc ctacgtgacc ggctgcagcg cttttcgatc gacctgtccg ccgtcgaaga
    30061 aaaagccgtg aaaggcagcg gaccgggcgg acaaaagatg aacaaaacat cgagcggcgt
    30121 cctgctccgc tatgtgcttt tgggccagcc gatcctcgtc aagtggacgc gcgaacggca
    30181 gcacagcctg aatcgttttt tggccctgcg tgagctgtgc gatgaagtcg aagcgatcgt
    30241 ctcgcccgaa acgtcggagc ggctgcgcga gcgagagcgg attcgcaagc agaaagaccg
    30301 cgtccggcgt cgatcgagcg atcccaaagc cgacccgcca gccgacaaaa gtacgtccaa
    30361 gcagtgatag gatgcgcggc catgcggaag gacgtttggc tgcttgtggc gcttttgtgc
    30421 ggcgcctgtc cgggcggcgc tcaggtcaaa agcggtgact ttgccgtcag cgagcgcctg
    30481 cgagacgaag actgcctgac tgcgagtgag ctgtacaaac agcggcttgc ggttccgcgg
    30541 cgtgccggtt ccatcgtgtc gagtcgcctg accgtgcgga gcgaagaaga gcaagcggca
    30601 gaccttttcg ggtttgttgg caagtgtcag acccagcttg cgggacgggc gcgacgcgcg
    30661 attgtgcgct gttggcagga ctcgaccgac gctgaaacgt tcgattcgtg caacgaccga
    30721 ttctgatcgt cggaaccgca cggcgaccca aacccctcgg agcgaatgga tgagcagcga
    30781 gacgatgcaa gcagtggtgc tggaaaagac ccccgaaggc ccaggccttg tgtaccgcag
    30841 tgtgccgcga ccgaccccag gtccgggtca agcccaagtc agcctgcacg ccgccgcgct
    30901 caaccgtcgg gatgtgtgga ttcggctggg ccgctacgcg cagatcaagc tgccgtcgat
    30961 cctcgggtcc gatggcgctg gcgtcgtcag ctccgttggt gaaggcgtaa gcccccagtg
    31021 gatcggcaag tccgtcgtcc tcaatcccgc gctcaccgac aacccgagcc cggacattca
    31081 ggggtccgat taccgcatct tgggcatgcc cgaagatggc acctacgccg aaagcatcgt
    31141 ggtgtctgcg cgtcgactgg tggtcatgcc ggcgcacctc agctttgtcg aggcggcggc
    31201 gctaccgctg gcgggggtga cagcctatcg gtcgctgtgt gtgcgcggtc aggtaaggcc
    31261 cggtcaaacc gtgctcatcc ccggcatcgg cagtggagtg tcgacgctga cgctgttgtt
    31321 tgcgctgcac ctgggggcca aagtggtggt cacgtcgggc agccatgaaa agctcgcgat
    31381 ggccaggcag ctcggcgcaa gctttggcgt gtcgtaccac gatgccgact gggacgatca
    31441 gattgccaag tttcttggcg gcgcaaacgt cgatgtcgtc gtcgatgggg caggggccga
    31501 cgcttgggca aagagcctga agcttctgcg ccccggcgga cggcttgtca gctacggggc
    31561 taccgctggg ctgccccaga tcgatctgcg caaggtgttt tggcgacagc tccagatcct
    31621 gggttcgacg atgggcaacg aaagtgagtt tgccgaaatg gtcgccttgg tggcggcggg
    31681 caagctgcgt ccgctcatcg atcaggtgta tccgctcgcc tctgcgcagc aggcccacct
    31741 gcggatggaa cacagccagc acatgggcaa gctggtgcta cagattcgcg actgatgctg
    31801 gacttgctcg cggcgataga acgctatttg gacgtgctgc gcgtcgagcg aggtctgtct
    31861 gcgcacaccc tttcggccta tgcgcgcgac cttcagtcgt ttaccgatta cttggccgaa
    31921 cacgatccgc aggcggcgac cgatgtgacc gcgctgtcgg cccggcatgg gctcggcttt
    31981 gcggtgagcc tggggcagcg gcagcttgcg cttcgctcgc aggcacggat gctgtcgagt
    32041 ctgcgtgggc ttggcaagtt tctccggcgg gaaaacctgg tgccaagcga tccgatggcc
    32101 gaagtcgcgc tgcccaaagt cggacggccc ttgccgcaca gcgtcggtca gaccgacatc
    32161 gaagcgctgc ttgcgcgtcc cgacgtgcag acgccgcgcg gggcacggga cgccgcgatg
    32221 atcgaagtgc tgtattcgac cggcctgcgg gtcagcgagc tgtgcaaact ggcgatcgcc
    32281 gatgtgggtc cgggctttgt acgtaccgtc ggcaaaggtt caaaaacccg gattgtgccg
    32341 ctgggtgagc gggcgcaagc ggcgattgca cattacgtgc agtttgcgcg gcccacgctg
    32401 ctgggccagc ggcagtcgcc gtcgctgttt gtgacccacc atggccgcgc gatgacccgt
    32461 cagggctttc acaaactgct cgccgcgtat ggtcgcgccg tcggtatcgc cgacctgcat
    32521 ccgcaccttc tgcgccactc gtttgcgacc catcttctgg accacggcgc cgatctgcgc
    32581 gcggtgcaga cgatgctggg gcattcggat gtgtcgacga cggaaatcta tacccacgtt
    32641 gcctcggcgt cgctgcgccg aacctaccgc cgccaccacc cacgcgccta accccaagcg
    32701 ctgggcccct gtggggttat gcgcagccac tccatcgggt accaaggcgg gaaaccaatg
    32761 tgcctgcggc tggctcacct gagggagcac agtccgcagg gctctagcta gttggtagat
    32821 ggagagcatc ttgggaagct cgattcaacg cggtggcgcc tttcgacggt cgtgctagaa
    32881 ctcgcccgca ggaggaacac atgtcggatt gcgcattcaa cccaagagcc gccttcgaac
    32941 aagcaagatt gggcactccc ttcgcctctt tgccgatccg ggggggcatc cgtgctggcg
    33001 atgcctcgac gtttcgctgc cacaccaaag ccaccttttc ccctggcaca gtgcgtgtct
    33061 acgacgtggc ggatcacatc gtcgcctggc tcgatggcac cgagccaacg agctggaccg
    33121 aggaaggctc cgtccactgt gatggcggca acggtgtcat tctgtccgcc gaagcactcg
    33181 cagagattga ggcactcgaa gatgagttcg aaatgttcga tcgcatagac gacgtcgacc
    33241 ttcccgccca agtgacgacg ccgggtggcc tgcccattgc tgccttcgcg tttctcggcg
    33301 atggcgtcta ctgcaccgcc atcggttgtg atgaggccgg tcagccttgc gcgctcgtgg
    33361 tccatgaccc taactagcta gacgagcgtt ccgcctttgg cccgtcgggc aaccctacat
    33421 ccgtcggaga aactgattct ttgagcttcc cacaaaaaaa tctgacagaa agcagctagt
    33481 ttggctatct ttgcctcgac taatcaatcg agggagaatg cacatggcaa tttacgctct
    33541 gtggaataac aaaggaggag tcggtaagag ctatcttacc tttcaacttg catgtgaata
    33601 tgcgcggaca cacccgacgc agaatgtgtt ggtaattgat gtatgtccac aggctaatgc
    33661 gtcaagcatg ctgcttggtg gtatgagaaa tggagaaatg gaacttgaca agcttggtag
    33721 cgcaaacccg cggctaacaa ttgcaggata tatcagagaa cgaatttcca gcccttacca
    33781 taatccagca aagggcgcga attatttgct acaagtgcag caggtgaaca aggctgttcc
    33841 tccgaacctg tatcttattc caggggacga agaattggaa gtattggcat ctcgtgtgac
    33901 aaacgcgaca tccccaggtc cggatgacgc atggtcgaag gttcatcttt ggctaagcga
    33961 tctgattcaa gatgtgagag ttagatggaa tgtgtctgcc atcaccgtct tcattgattg
    34021 taaccctagc tttgggatct atacagaact tgctatgtct gcatcagatc gcttgataat
    34081 accgttttcg gcggatggtt cttcgcgccg tgcagtcaga agcgtattag cgcttcttta
    34141 tggagtgact cgtgcacctg gaggccaaca gtcagagtat tttattaaat cacagcgctt
    34201 ccgtttagcc gtcccaaaga tttattgcta tgttggaaac cgattgactc aggctaattt
    34261 tgcatcagca aacgcattta agactgttgt cgatgccatc gggaatgaaa tatggcaaat
    34321 atggataaat aatcctggca atttctatgt gcatccgaca ggtgaaccac caccttcgac
    34381 ccggagcgca ttcaagagaa tgttccaata tgaagtcgtt gatgcgaata cggcctccgt
    34441 cgtatctcag gcgcttggta ttcccatcgt tacgcttaca gcgggtagca aacgattggt
    34501 tggaaaaacg gcaatcgtta atcagactca actcaacaag cagcagccga acatcaaagc
    34561 acttgttgcg acgatagagt gaacggcgcg gtgcagacga tgctggggca ttcggatgtg
    34621 tcgacgacgg aaatctatac ccacgttgcc tcggcgtcgc tgcgccgaac ctaccgccgc
    34681 caccacccac gcgcctaacc caacgccctg ggcccctgtg gggttatgcg cagccacggt
    34741 cacggcgtgc ggacccacgg tcacggcgtg cggacccacg gtcacggcgt gcggacccac
    34801 ggtcacggc
//
LOCUS       DAORGN010000024        34424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_456_length_34424_cov_5.588204, whole genome shotgun sequence.
ACCESSION   DAORGN010000024 DAORGN010000000
VERSION     DAORGN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 34424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34424
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_34424_cov_5.588204"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..609)
                     /locus_tag="PKL17_04670"
     CDS             complement(109..609)
                     /locus_tag="PKL17_04670"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNK44052.1"
                     /translation="MQFRFVHDFDVEPAGFWEMFFSEAFENELFRGLKMKTWKVLERR
                     DEASKLHRTVKLEPDMAVPSWATSVVKETGYTEIDVFHKAESKMDVKIDPAVMRDRFQ
                     LSGVFAVTPLGPGRCRREFSGEIKVSVPLLGGKIEKFMIDQIRDGYDQAARITRDYLA
                     KQKSQS"
     gene            complement(639..917)
                     /locus_tag="PKL17_04675"
     CDS             complement(639..917)
                     /locus_tag="PKL17_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HNK44053.1"
                     /translation="MPKVEISHKHTVTAAEAKQKLDQLSKDLSDKYGLTSKWVSDTEA
                     KVERSGASGTIRIEPSLIKVAIDLSFALTPIKGTVEEKIKKELAKLFG"
     gene            1146..2150
                     /gene="selD"
                     /locus_tag="PKL17_04680"
                     /pseudo
     CDS             1146..2150
                     /gene="selD"
                     /locus_tag="PKL17_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            2283..4400
                     /locus_tag="PKL17_04685"
     CDS             2283..4400
                     /locus_tag="PKL17_04685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNK44054.1"
                     /translation="MQLDSEQALRIATERYRSWLRSIADVAKVVFILVESAGNLIEIV
                     SDFALQNRLGIEQFEPGTPLQQVVPPLAESLLQGLAICRHTGHSQIIEHTIDDSQGVL
                     LLKTLITRHQHNSYAIAIKNAHIERSLALQLESIASDQRQTVALLHEMDSRYRTLIDS
                     LSEGVVFISREGQILAANEAAAQLFRRSVADICKWRFGDPLWFQVIGEDLQPMPMDRW
                     PLFVTLDSGQPRQAMVAGIVFPSGNPLWLEVNSRPLLRPGEAKPYAVVLSFFDITVRK
                     QLAAELLHRAFHDPLTALPNRSLFVDRLDTAIRQARRTGDLVAVFFLDLDGFKEINDA
                     YGHETGDRFLQGVAHRLSNSLRDGDTVARFGGDEFTILLPSLQTAEDALRVAERVLEE
                     LQRPMQIDRVSLKAAASMGISLYPHDGQESATLLRNADQAMYRVKSNGRNGCQFFRTE
                     MNLTLVRRPPREEARVSALTAGVLRLRYRPLVELASEQVVGWMAELYRQIAGTRPEDC
                     PSEASLGDELAQQIFQGLVEQGCRELSTLAAGRSDAPLVVRIDGRHLVGNAVLLSVTG
                     ALEKTGLAAHLLELDVLAPNSLVDGAALTESLWQLHRLGVRLSLSASPLSAMPLLQLS
                     QLPLTALTLPEPLWRRSMEDKRTASFCEGLLAIADRLGFLVSATGIRTQAELRHVKQL
                     GCQRGQGSLWTLGSQPPKREDSA"
     gene            complement(4411..4845)
                     /locus_tag="PKL17_04690"
     CDS             complement(4411..4845)
                     /locus_tag="PKL17_04690"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK44055.1"
                     /translation="MANGPLWRTASRWLGLALSTLLVACLEQPADLKASVELLTTSSG
                     EVQEQALRTLLAHGRAALPALEAVLHRPDPRARHAGVVALRRLGLAETAPLLGHVAQF
                     DPDLGIRREARTQLEKWAAEKSARGESAQKALFLAAPGDVNQ"
     gene            complement(4845..5822)
                     /locus_tag="PKL17_04695"
     CDS             complement(4845..5822)
                     /locus_tag="PKL17_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK44056.1"
                     /translation="MANVELRDAHVLITGGAGFIGSHLVERLCQHNRVRVLDIMRRDA
                     LRPAGLHDHKNVELVVGDVLDPVAVQKAVKDTDVVIHLASIAGVDTVMRMPTQTMRVS
                     LLGTAFLLDAAHAGGRCRRFLDFSTSEVFGRYAYQVSELDATTVGAVGEARWTYAVSK
                     LATEHLAMTYHRQFGFPAVSVRPFNIYGPRQIGEGAVHHFIVRALKGEPLHIHNDGSQ
                     IRAWCYIDDIIDATLLTLSVPEASGQAFNIGNPRSTVTVYHLAKLVKELCKSSSPIEF
                     VRWDHPDVELRVPSVGKARELLKWEPRFDLEEGLGATIAWYRQKLGITG"
     gene            complement(5854..7236)
                     /locus_tag="PKL17_04700"
     CDS             complement(5854..7236)
                     /locus_tag="PKL17_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNK44057.1"
                     /translation="MFSRLEKPKPVVEHAAKVDPGTPLAERLRPRTIEEFIGQDHLLG
                     EGKLLRQAIRSKQLPSLVLWGPPGTGKTTLARILAGAVGARFSALSAVTAGVKEVRAA
                     IAEADYERSHGGKTVLFLDEIHRFNKAQQDALLPHVEHGTIVLIGATTENPSFEVNAA
                     LLSRCRVVQLFALTQVQLGQLLDRAISDRERGLGSEPLCLTEEARDFLAEHSGGDGRR
                     ALGVLEVAALHASRVDEQGRRQIDRQSIAEALQRKVLLYDKTGDEHYNVVSAFIKSLR
                     GSDPDAAVYWMARMLEAGEDPLFVLRRMVIFAAEDIGVADPRALQVAVAATEAVRFIG
                     LPEGTLPMSEAAIYLALAPKSNSAIATYQAAKRAVEQHGALPVPLHLRNAPTKLTKEL
                     GYGKDYKYPHGYAGHHVAEHYLPDALTGQHFYAPSQSGLERALAERLAQLRGQPDGAG
                     SQDNKKTGKA"
     gene            complement(7298..11053)
                     /locus_tag="PKL17_04705"
     CDS             complement(7298..11053)
                     /locus_tag="PKL17_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44058.1"
                     /translation="MAGGDRTSSYVGIMLAVLATVVVPGTIVAGLGTGAEGKPAAPAP
                     ASALKNDAAPDKKDAAPSQRGSHKLLCDLGGNVEPGCQTGSQPPDPLSPPRRSYAGTV
                     SALFVTVPEPSWGLDGMLEAMVRSFETVGYTLRAQDLPRVTGKDSPLAYGVLVFTGQN
                     TDAYNPPTRMMRKEATPKLPSGGAKQAPVPAAEPPKPFSPENAYVVYLIEETPTAGIR
                     LAALGEALGDWDSLRQEYGPPPQDEPLALLAPAFSGSAAGLAHFIASHELWRKYPLEI
                     ISGRATSTDVAQALRTARRSGPQVRFHSTVHSDEDMQEAMFSFLVDRLGADCRKIALI
                     VESSTPYGNATYQQDRSLHRGRVRRSHACPPRLLPVPSDLPRLQAEWERKKNTTQTPS
                     AATYEELAKNSLERQRDDSRRTGMLPLFSKETLSERDLSLSALLSTLYQEGIRYVGLM
                     MTDASDKIFLAQRISANCPDARLFTFESELSLVHPQDLAYTRGMLVASTYPLFTRNQQ
                     WTYPYSGAMQRLQFASQADQGVYNAALLLLGHPEHLLEYAAPFAPFERWESYAKLWRC
                     TRPSAQDPLCLAKPPVWISAVGRSALVPLEVSAPSDDDFVADRRHALFRAGEKTPLRD
                     TITKIPNSEQRYTPYDMGTVRVLTIVLLLLSLSLGVPFLRNAYGTPLWVSEAGEEARF
                     FDFLKPLPQSPHPLFAKEHTARSVYLLWLSLPLGVLSFYLGLLLALRFRAGNFMQDGT
                     GLLGRFFGNALFDSTTDWSKILDLLYLLMSTTSQSVSWVVTICVLCRMWNRPPRWPRL
                     RRFLTSLGFLSAATLFLSLVALSIAINTVSALDTKDLTSWHGANAILFLRRSAQTSYE
                     LSPLLPLLCLLAISFLWGLYNLRRISLVTLRPLSDLPVYSPPDKAQQIQQDMRLCYAQ
                     LGSHHRLTVVSVVLLVVLVASPVFGRLTTIDMSAVHLLFRVLFSVATSLIVLAVFRLL
                     LLLWPLEGALHHLRHHPLASALSRIPAAWRRPLGTMLLEELSGATEQQMLEQKLDALA
                     AGLAQQTPQARRQAIPVELHESLSLLEDRVATWHNTNPPPDPMSLVDVDLERAASALL
                     YGTRPPLRPRPAPTAPPDAGAKEPPAPDPLAFLPLAEDLLSTRLVLILGRLLPHLRNS
                     MLVTTSCMVLMLAALWSYPFQPMQWMTILIWLIFLSVCGLTAMVLLQLNRSPILSQLT
                     GTEPGKLSWDTSFLRPVVYYVLIPLLSLLSTQLPEFNWLVKLLQNLK"
     gene            complement(11069..12520)
                     /locus_tag="PKL17_04710"
     CDS             complement(11069..12520)
                     /locus_tag="PKL17_04710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK44059.1"
                     /translation="MTMTRLSMRIADPFMSHLAAPALAPTAAAEPPCLREGPPVPYGT
                     AEAQTPLWIKKPTAQPDQPWILRRRDGIEIILERKLRLGTAEDNDWVLHDSYVSQHHA
                     LLTASEDRVLVQDCGSRNGTFVNGVRLQKGELLDGGVLRLGETELWLARKPQPHSMGL
                     VGVSPQMLAVRKQIARVAPSSLPVLILGESGTGKELVAKAIHEASQRNGPLVPINCGA
                     LPRELIESELFGHERGAFTGADRKHLGCFAEAAGGTLFLDEVGELPIELQPRLLRALE
                     TQKIRPVGSTREVSVDVRIVAATHRNLPQAVADGRFRADLYYRLAGLVIDLPPLRERP
                     TDIPLLVLHFLTEVAVQHPGLHIDDDQIQAIMQAPWLGNVRELRMALLRAAHLHGPTL
                     RARDIIGQQKPLPSSSDSQVQVVGRRLVDIERDVLAQVLRHSGGNQRTAALVLDVPKS
                     SLADRLRRHGIFTSREVRVDSPTAKEPKRGTMT"
     gene            12678..13580
                     /locus_tag="PKL17_04715"
     CDS             12678..13580
                     /locus_tag="PKL17_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNK44060.1"
                     /translation="MSDLLIRAIHRTLNVRIVAAISTEVCKDAARRHRATPASACALG
                     RGLLGAYLLATLTRGGERVTIQIQANGPLRGLTADAYDDGSIRGYPLAPEACPTAKMD
                     RRQRMADLIGRDGVVNVVRDVGLKERFQGQVSLIVSEVDEDIESYLRQSEQIPSALGT
                     ELIFDSGGAIIQAGGVLIQSMPDSPDEVKTVIREAQHLLRAGELYDLLAAGPQTPESL
                     AKLVLGPYADGLEVQDTRELQFRCRCDRERVAAMLRGLGEMELDEMIAEGKAEITCNF
                     CADVYQFSVDELIEIRRSKQKVTN"
     gene            13589..14506
                     /locus_tag="PKL17_04720"
     CDS             13589..14506
                     /locus_tag="PKL17_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNK44061.1"
                     /translation="MSHFLCVAPEVAHALAEGRAVVALESTLLSHGLPWPDNLDVALK
                     AEAAVRQSGAVPATIAVLGGRIHIGLSMAQLEQVGQGQGLFKASAADLGPLLAAGVDG
                     ATTVSATVVAAARAGISVMATGGIGGVHRGDGFDISCDLTTLGSEAVAVVSAGAKAIL
                     DLPKTLEYLETLGVPVVGYGTAELPAFYTRKSGLRLLHRVDGPEPAARLIDSHFAVHS
                     RRGVLLCNPIPDAEALPSELVDRALQAALSAAETAQVGGKALTPFLLARIAAETGLRS
                     VRANCALIVDNARVGGEVAVALSAQRKAA"
     gene            14547..15428
                     /locus_tag="PKL17_04725"
     CDS             14547..15428
                     /locus_tag="PKL17_04725"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNK44062.1"
                     /translation="MSRKQIFTVASADPRGLLALLSDRLGLSLAAAQQAVVEGSVFVD
                     GRRVNKTPPEVLPGQKVQVFVPDEPPVPKSPRIVFEDADLLVVDKPAGLLCQPGRRGG
                     PSLLTLLPGPLWLPHRLDADTSGLTMLARSAAVCARLGQALAADQVTRTYAARACGLV
                     PERGQVALRIGKAAGPAVVKMRTYPPQSTQGDPALTSFVRRAVSDSSQGADSLVWLRL
                     STGRTHQVRVHLAGIGHPIVGDSLYGGRQASRLLLHAVELSFSHPRTGQLVKLASPLP
                     AEFWPLPVDDALPPHFG"
     gene            complement(15435..18041)
                     /locus_tag="PKL17_04730"
     CDS             complement(15435..18041)
                     /locus_tag="PKL17_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK44063.1"
                     /translation="MRRWLLGLPLSMLLSGSVLAADLYGGQSDLPDEVRTLLQASDPQ
                     RRKEGIERLQALPQRLSSPYLVQRLHDGEPMVRARAAQALGPIGSLAAIGPLLAGMSD
                     ADSTVRAASAEAIGQYGALPKAEQQRAIALLARAMGDGQFEVRQEALRAVERLLFSQV
                     ITVADLPPLMGPLLLRAEDENVSVRRCAMAVLGRLSPLSLSADQRGRVLLSLLGRLSD
                     PARDVRAESLRSLGLLRADSAIPAALRLLRDPSDEVRKQAMLCLGRLSAESAIPLLRE
                     TLETGSDSQRASAVQALSFYLLPTSPKRLSADVLALATQAILTSLGNPALRPLVVEAL
                     VAAGDRVGPILLQELARPALDLAQLATLIELVRDLGNKLPPPDRRTAEQVLSRELSQN
                     RVPKELLLDALAAIGDRNTAALFASLLTDKDVIVRRRAVQLLRKPNLLDVRAKDALLA
                     ATSDADPHVQRQALLALGDLQQGQARLCEVLDPTDRPALPDAETRLAAALAMQALASS
                     GALDEKSVSILVRAVLTMRPGEDERRIRRRSAQALASQLGPHPEKQGPIVAELLQALR
                     KPPDGGPHSEVLLLLSGLLRGHGSETVRERLLALSQLGAEPHSAEGQLAVDALAAIQS
                     FVDLPAQSRLIRLLSHGDPLRVNRATAALGVLLSATPTENAISALVAQLSDGGDLRTL
                     AETAWALSNLPRNHPACGRVVERLRAMLQIRRESSPDLTALRCNVLGALARLGAAETS
                     DLQWLDDPDPGIRRNAALLAGLLVPRAGTVESRLRSASASDEDRAVRQNAKAALLGQG
                     AHGHLSRKHWIITYQFDLDGKPRSFLPYRLVMGDGINRVGYTDRTGTAVDEHLPDGDN
                     EVELIAPPVTPR"
     gene            complement(18107..18826)
                     /locus_tag="PKL17_04735"
     CDS             complement(18107..18826)
                     /locus_tag="PKL17_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK44064.1"
                     /translation="MRCERCCLEDPACDALDADAFARKLSGCDSCPLLQGASPIPATV
                     LSKQLQRIKESARLLRHKQNRIRQLEHAQKELTQDILRSDERVSKLERTQHAIAQAAD
                     AQLQSYLRMLESQQESIVALSTPIIQVWDAVLVLPLIGHLDASRLQTLTENLLASLQQ
                     HKARFAILDLTGISELDAESAGRLLKVAQAVGLLGATALLCGLRPQVVRTVTSLGVDL
                     GELSVTRTLKEALGHCIARVD"
     gene            complement(18839..20017)
                     /locus_tag="PKL17_04740"
     CDS             complement(18839..20017)
                     /locus_tag="PKL17_04740"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNK44065.1"
                     /translation="MVWSGSHVATVSIAGDDAHEAAKEAAFELKDRLGCTPDVVVVFF
                     SAEFAAAEIRNGLWSELPSTTQVLGCSSFAEIGPDGALTHSVTALGLVLPGYRASVLS
                     VAGGSDSYELGKQLVFPLSGQVPDLLILLPDVLSLNATRLLRGIQKVIGDRTPIVGGA
                     PADMGAFKQTTMIAGSNLQTSGAVALALYGPISISAAAFSGYTPVSIPLRATKVEQGN
                     VVLELNGKPALDVYLDFLGPRKSELPAVTIEYPIGVLSGPADAKTVTVDVVRAAFRMD
                     ENRGAIVLGGDIPEGAELRILSATRDDVLRGTKQAMQSALLSCQDADLMLLFNCMSRK
                     IILGPRYKEEYVVADSVLPKEVPRIGFYTYGELSPVAEVTQHHESTFTVAAIRFGKHS
                     "
     gene            complement(20128..21471)
                     /locus_tag="PKL17_04745"
     CDS             complement(20128..21471)
                     /locus_tag="PKL17_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44066.1"
                     /translation="MIRISPGSPRPRSQHVSTAWSVSQSVSLSVSLAASLAASLAVSV
                     PALLSGCGDSHTNTLPPVPPPPGVTKKTGMTYYKDIQPIMQRSCQGCHVVGGVGPFPL
                     TTYDEALQNAAQVSSAVVARRMPPWKPADNCQKFQNARSLGQDAIDTVYSWVADGSPA
                     GNPADAPPNMTPNVVTLDWVDATVSPAASYTPAANLTTDYHCFVLDPKLAASRDVIGY
                     EVTPGAKQNVHHVLLYPADAATASAQDAKTPEVGWPCYGGPGISTASTIGGWVPGSPP
                     TKYPTNTGITLAAGQVLVMQIHYDNHHGGAWSPDQTSVKIQFAKTPVAKKATVLSVRN
                     ASFSIPSGATDYAVVGTMTTPSDGLIYGLAPHAHQLGKRLRVETADTCLIDIPSWDFN
                     WQQFYFYDGSQGLPFPKGTQIKLTCVYDNNTASPVGWGESTTDEMCLNYFYATGP"
     gene            complement(21546..23555)
                     /locus_tag="PKL17_04750"
     CDS             complement(21546..23555)
                     /locus_tag="PKL17_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44067.1"
                     /translation="MRHVVKLCGTLLLSTALLVGCESLWIGYKTPNPLSCEAPGYGCP
                     SDQVCNPQTRQCEPIATTMLDLAMSSGSDGGTPSSCTGAVAFACTSGSGSGMFCTQAP
                     LSMGPLKRLHGTSESDIWAISQTRLARYNGTYWNPVTTCAATTGFVDVFSVSPSDVWF
                     IEDRRVYRLLSQTLSEVPITIPAGVSLRAIYGISGKIFVAGSAGFLQKWDGTKWIDQS
                     PGGTAALSSIWGPDEQNLWVVGESGTARYWNGTAWTPLPTGITTNLDSVFGSNASNVF
                     ASGPSGALFQLTSSTATAVPSGTTAWLVAGSLAGDGTGFVVGDKVTLRRSGGFSLLSP
                     TEPPSKRMNTDVQAFSSNAAWAIGGSTGAVRSKWNGTLWFDSLDEILPVVRSLGASSQ
                     TTPSTVFAIGAGSDGKTYALTTDFVSTSKVVGLGGAGFVGNAVWVDPAPTGTTTAWAV
                     GSGGGVYSYTSNIWTAASSGTSMDLYGICGASANQVVAVGHDAAEANGQMTGFNGSSW
                     TAGGIITGSPLYDVACAGINVMAVGKDARVYDCASTCIANANFPPITGQTGYAIWITP
                     SASGMGQDIWVAGSNGTLFRHQTGTNSRTPYTVTTQHLRHIFGKNHSDMYVVGDGGTV
                     LKWNGSSFSVVKTDTAQRLQAGTVFPSGNSAFIGGPMAGWLHQIP"
     gene            complement(23579..25054)
                     /locus_tag="PKL17_04755"
     CDS             complement(23579..25054)
                     /locus_tag="PKL17_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44068.1"
                     /translation="MSNLPYVASRLFAAWLLVGAAQPARAQIDARTRVRDLIAQAEQA
                     RSAHDPAQAEKLLEQALRIENQPAITLQLGKLAEQEGRTAAAADLYRRYLDEMGESVD
                     ETTKTRLTGLISALHEPVAEVDVLGPPGSLLYVDDHLVGMLPLSGPMLLLPTSHRFRL
                     IIKNSAYRSDPLDLLPSLRAQLHLTPGAGGTAVAILSLSSEWLLLIEPADMDPGQRSI
                     VEQSVSSLAKRERATLLSTQRLERAKKSRPADCLRDPACQESVARDLGVRAVVRVHVD
                     QAAGKIRAEWFDVDGGGVAAQRERACGNCQEKAMREAAALLSRELLRDAQNHPRGVLD
                     VSSTPPGAQVVIDGSVRGETPYQRALLVGHHNLKLRLPGYSDYDEPVELLQGQTRQIA
                     ATLTAGTGTPLAPQTNQPPQPRPLWRYLVGGGLVVSGFLLSGFGANALSQNGTCGDTT
                     APPAGAPCNYLYNTGSVGGGLLGAGLGLAAGGALMIAWPTK"
     gene            25128..26495
                     /locus_tag="PKL17_04760"
     CDS             25128..26495
                     /locus_tag="PKL17_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNK44069.1"
                     /translation="MLPLPAMEPLAGLRTEAQIHGFPTVIDLPKVRVAVVEGPDAGKH
                     FDSDDEMLVRIGTRENNGLVLSDKSVSGYHMEITGTQSGWRLRDVGSTNGTFVAGMRV
                     FDLVLRSGTVVALGRSKLRVDAIHDEPVRAALHPAGRFGDMVGQSVVMRRLFSRIAQV
                     ARANSTVLITGETGTGKEAVAEALVQASLRASAPFVVIDCSALPHNLIESELFGHTKG
                     AFTGAVSDYRGAFERADGGTIFLDEIGELPLDLQPKLLRVLERREVRRIGAERTRSTD
                     VRVIAATNRVLATEVNERRFRQDLYYRLSVVNVHIPPLRDRREDIPLLIAHFYDRLAD
                     AHRGPSLGEVTQRLCDNDYTWPGNVRELRNAVERAFHVPDEDPFFAGGTPLRADASSE
                     AVHVDLAVPFKTAKQKLIDAFEKEYLQAMLKATRGNISEAARRAQIDRMHLYKLITQH
                     HLNGE"
     gene            complement(26554..26865)
                     /locus_tag="PKL17_04765"
     CDS             complement(26554..26865)
                     /locus_tag="PKL17_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44070.1"
                     /translation="MPRRLVQNLSPRIGGALLIGTLLLFASQLRCADANNFIEDDDPA
                     LTGDGGVQKGDLAEGPCFMGTPQTEPNLLNRCSDAERIERGSKIPASLWDGQSPLPYE
                     K"
     gene            complement(26865..28067)
                     /locus_tag="PKL17_04770"
     CDS             complement(26865..28067)
                     /locus_tag="PKL17_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44071.1"
                     /translation="MSVRHLSVLLFVAAAALPGRAFALQCSTLPEPTYLTATPSAEPL
                     VEKVASLLQRTDAQRTTVIYQIRPSCTALESLVKDTLPGSCASDACMRGKAMFFTLDP
                     RDTAPKECDLDTTGNKIDIVLSDVFPRSCPSYTQTAPTGIIDTAGPVSSYSLAMLRSA
                     TESAIQAEEAHFVFGAGAAAKIKPWMNDAAIGHLGTQDAGTLLVGGRIKLANSRWKGT
                     TAGSIDDLINIIYNDGTQGIGIVPTTVADRRRAELRPLGFQAIGQKGAFFPDRRSTTY
                     DKQSVRDGHYPLWGFLHTILRQDPGNPGQPLSKKGARLANIFLGKTQVGGQDSQLLQV
                     QSGFIPQCAMRVTKTSDGAPMTVYAPGEPCHCFFEHNVSQGALSCQQCPTGTCSSGVC
                     RRKYCEAQ"
     gene            28363..29334
                     /locus_tag="PKL17_04775"
     CDS             28363..29334
                     /locus_tag="PKL17_04775"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK44072.1"
                     /translation="MAASSKTRPYISSRFVLELDDSGSGQYMGTLQSIEGGTFKSEVL
                     EEKVGGSGAVTKYPGRPKFEDVTIQVGMTMAPRFWRWLAKSFTYDAQRVDGSLVALDF
                     DNRERWRRRFSGALIREVTIPTLDGAAKEPAYLTVKFAVETIKEEQSGNNKYPGEQQY
                     KDEWERQRMWLPSMFTFKVDGISRDPAETCKIDSFSIKQEIIENPIGKFLENQKEPGR
                     VEFPTIGVTILQRDAGPWMKWWDALVREGKHVLSQQHTGHIWFLPRDAVGSGQLQRKP
                     LLTLDLFGVTILGLGPVKHDSKQNQLQKVKLDLACERIDLKGGEGTV"
     gene            29421..30668
                     /locus_tag="PKL17_04780"
     CDS             29421..30668
                     /locus_tag="PKL17_04780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44073.1"
                     /translation="MARRKLTNIGNYRLVREIGRGGMGTVYEAEHERVKNRVAIKVLH
                     PEYCKDPQIVARFRQEPMAANLTRHAGITHVFESDVLPDGTPYLVMEFLDGQSLRMRL
                     RKARGGLPQLQVIRFARQLADALGAAHSKKIIHRDIKPENIMLVRDDAVPGGERAKVL
                     DFGIAVVAEDASLSMSSSSNTQIKTSPFASLLGTAIYMAPEQCLESGRAVVDEKTDVY
                     GLGIVIYESLCGQAPFVAPEMVSVMHKHISEPPPALSLLRADVRPELESLVMRMLAKS
                     GSERPSMREVSAQLQSIELAEADPSVGLSVPPPPSSMGSRLWLGLVLLFVLAGAGTLF
                     FFGRASSGTVTWRIDSSPSGAELVNAAGEVIGKTPYRHGPKRDVGKLAVMLRLPGYFP
                     ETVTLDYEHDSKQLVQLRKKPPQ"
     gene            30679..33189
                     /gene="sppA"
                     /locus_tag="PKL17_04785"
     CDS             30679..33189
                     /gene="sppA"
                     /locus_tag="PKL17_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNK44074.1"
                     /translation="MQTTGLRGLGVVAAIAFLGISGSHAQVPVRRSPIDSVYSPGMGV
                     ADGAEPWAVTLNPASLGGLRGHTFGLRHSELTSQIPWSGRGTGAYFASPLPYLSAFKV
                     GGGLEVLRPHDDLSILGKLTVAMAYQPVWWASVGLLYGHTFGKPGDRSYAGLDTVSFG
                     LHLRGNRRLAFGAMFHDLNAPKPTDPAWLPQVSRSYELEALLQPLGDSRWDIGLGVRI
                     GEATPELSPRLRLWVRPTAGLGIGIEGSSVIIPGSGDPVAYRIGFGLGIDFSRIGVSV
                     FQLAGYGSDAPLAYHGGSVAVRMSQERYPALWSGPRQVLRLHLGEVSDRALLKLLLAL
                     RDQKQSQQIEGVLVIVSGVSGSWATAYELRQALVELRKAGKRVIAYGADLGTREYYIA
                     SAADEILLDPVGLVRLSGIAQGTTHYREALLRLGIRADLVRIGRFKASPESFTRDGPT
                     ELTRLQRQSVVDDLSQRVLGEIAESRHLPLGDVERLVERGQQLPQLAKSARLVDAIAS
                     QEELQKRLVQLYGPSVRVANLPREPRSPSARPHGVAVIEVSGDLTDGQSFALPYVDFR
                     TVGAQSLSAAIERAAQDPRIEAVVLRVDSPGGSALAADMLARQIKQLAQQKPVICSFG
                     DLAASGGYYLSAPCREIFADPLTITGSIGIFGGKVDVSGLLAKFGVERARYTSGPHAD
                     MESPFRAYSDEERELLRERLQAGYDRFLDVVESGRKLSRKELEPLAEGRVFSGGQAAA
                     GRLVDKLGGLADAVARARELAGLAGENDAAVIFLPDRPRSLLRELVDVAGGLTQESTQ
                     LLPGPLRELIRLVPPMLWALSFDGSDVLLRLDEEPVKP"
     gene            complement(33186..33938)
                     /locus_tag="PKL17_04790"
     CDS             complement(33186..33938)
                     /locus_tag="PKL17_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNK44075.1"
                     /translation="MSGSKPAKEPALRERLALVCQRLYQKGLIAAGDGNVSLRLDDER
                     VLVTPSGFHKGFILPDELIVTDLQGRRLRGAHNPSSEFAMHALVYAERPDVAAVVHAH
                     PPITVALALAGVSLTDCVLSETCLLLGSVPTAPYSTPTTSEVPESLRPYVRKANAIVM
                     HRHGALTMGRTVEEAWQRMEAVEHSAKIIHAARAIGEVVPLPPPEAKKLDQLAQRLGI
                     PRPPEPPSIAPSERSEAAIIEAVLRKLATGQA"
     gene            complement(33951..>34424)
                     /locus_tag="PKL17_04795"
     CDS             complement(33951..>34424)
                     /locus_tag="PKL17_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK44076.1"
                     /translation="ELDQIALSDEVALFLATHIKSNVRELEGLLIRVAAFASLQQRPI
                     TIEFAQDTLRNFLTQSAAHLNVEAVQREVASYFGVKIADLKGSSRLSSVTKPRQVAMY
                     LARKLCKASFPELGLKFGGKDHTTVMSACRKVEALLDTDPQLRGMVQEIERHLLA"
BASE COUNT         6299 a        10091 c        11354 g         6680 t
ORIGIN      
        1 acgagcgctg ccgagcggga cgccgcagtt tcgatgtacg gttatcagtc agagggcggg
       61 gtcggctatg tgttccccgc gccgtgacgc acggggaatc gggcggggct acgactggct
      121 tttctgcttg gcgaggtagt cgcgggtgat gcgcgcggct tggtcgtagc catcgcggat
      181 ctggtcgatc atgaattttt cgatcttgcc gccgagcagc ggcaccgaga ccttgatctc
      241 accggaaaac tcgcggcggc agcggcccgg tccgagcggc gtcaccgcga acaccccaga
      301 taactggaag cggtcacgca tcaccgccgg gtcgatcttg acgtccatct tggattcggc
      361 cttgtgaaag acatcgatct cggtgtagcc ggtttccttg accaccgacg tggcccaaga
      421 tggcactgcc atgtcgggtt caagctttac ggtgcgatgc agcttgcttg cttcatcccg
      481 gcgctctagc actttccacg tcttcatctt gagtccgcgg aacagctcat tttcgaacgc
      541 ttcggaaaag aacatctccc agaagccggc tggctcaacg tcaaagtcgt gaacaaagcg
      601 gaactgcatc aaggcacctc gtaggaagcg gcttggcgtt agccgaacag cttggccagc
      661 tctttcttga tcttctcttc gaccgttccc ttgatcggcg tcagggcgaa cgacaggtcg
      721 atggcgactt tgatgaggct cggctcaatg cggatggtgc cgctggcgcc cgagcgctcg
      781 accttggcct cggtatcaga gacccacttc gaggtcagcc cgtacttgtc cgacaaatcc
      841 ttgctgagct gatcgagctt ctgtttcgct tcggcggcgg tgacagtgtg cttgtgggaa
      901 atctcaacct tgggcatcgg cgtgtgctcc ttcttgtgat ggcgttgcaa ggaccgcagg
      961 gcggggcgtc aagctgcttc gtggcaggtc gacgtccaaa tcttcgccgc tacgtgcgct
     1021 atatcatgtg ctagcttgcc gccaaaatgg acaagttaaa aggcccaggc agcgaggtag
     1081 aaaagccccc tgcctttcgc atcacccggt tcgcttcggc gggtggttga gcggccaaag
     1141 cgggtccggg cgacctgcac gcacttttgc acgcagattc agcgacccga cttggtgccg
     1201 caagccagcc cagtgtgctg gtggggaccg aaacgggcga cgatgccggg gtgtatcttg
     1261 tccacgcccc aggcgacgcg ggcattgggc ttgtccagac cgtggacttt atcacgccga
     1321 tcgtcgatga cccggtgctg tacgggcaga ttgctgctgc gaactcgctg tcggatgtct
     1381 acgcgatggg tggcaggccg atttcggcgc tgaacgtctg cgtgtttccc aaagagctag
     1441 aacccgaggc ggcgcaaggc atcctggccg gcagtagcca aaaactggcc gaagccgggg
     1501 cggcgctgct cggtggccac acggtgcgcg ggccggagct tctgtttggg ctatcggtga
     1561 caggtcttgt cgacccccag cggatctggc gcaacgtcgg ggcgcgcgcg ggcgatgcgc
     1621 tgattgtgac caaaccgctc ggggccgggc tgttggcgac gggttatcgg cgtgggcttt
     1681 tgtcaagcga cgagcttgca ccctggctat cggggctgtg tcagctaaac cagcgggcgg
     1741 cccatttgct gggtcggttt tcggtgtcgg cagccacgga cgtgacgggc tttggccttg
     1801 tcggtcacag tctgggcatg acggcgcggg gggatgtgtc gctgcgtatc gaaagccaca
     1861 ccctgccggt ctatccgggg gcgctgcggc tggccgatct gggggtgacg tgtggcgggg
     1921 cgcgcaacaa ccagcgggcg ttttcggcgc aggtccagat cgatgaatcg gtgacggcgg
     1981 cttgccgcga gctggtcttt gatccgcaga catccggggg cctgcttgtc gcgctgcccg
     2041 agtcggtggt cgaggccgcg cttgcggggc tggccgacga aggcatcggg gcggcgcgga
     2101 ttggtacggt ccttccggcg ggtaaaaagc ctatattgat atgtacttga cctgtgtggg
     2161 cgaaggcggt ccggtgctgc cctggcctac atctgttagg tgacaagatt tcgtaacttt
     2221 tgcgacaaaa gtagccctgc tccatccgta taatgggtac gtcatgagca tggagacggc
     2281 ttatgcagct tgactccgaa caggcgctgc ggatcgcaac cgagcgctat cggagctggc
     2341 ttcggtccat cgcagatgtg gccaaggttg tcttcattct cgtcgagagt gcaggcaatt
     2401 tgatcgagat cgtgtccgat tttgcactgc aaaatcgctt gggaatcgag cagtttgaac
     2461 ccggtacacc tcttcagcag gtcgtcccac cactggcgga gtcgttgctg caaggcttgg
     2521 cgatttgtcg acacaccggt cacagccaaa taattgagca tacgattgat gattcacaag
     2581 gggttttgct gctaaagaca ctgattacac gtcatcagca caactcatac gctatagcga
     2641 taaagaatgc acacatcgag cgctcgctgg cgctacagct ggaatcgata gcatctgatc
     2701 agcggcagac ggttgcgctg cttcacgaga tggattcccg ctatcgcacg ctgatcgatt
     2761 cgctgagcga aggcgtcgta ttcatctcgc gggaaggtca aatcctggct gccaatgagg
     2821 cggcggccca gcttttccgc aggagtgtcg cggacatttg caagtggcga ttcggcgatc
     2881 cgctgtggtt tcaagtgata ggtgaagacc ttcagccgat gccgatggat cgctggccgc
     2941 tgtttgtgac actcgatagt ggacagccgc gccaagcgat ggtggcaggg attgtttttc
     3001 cttcgggcaa tccgctgtgg ctggaagtca actctcggcc ccttctgcgt ccaggggaag
     3061 ccaagccgta tgcggttgtg ctgtcgtttt tcgatatcac ggtacgcaaa cagcttgcgg
     3121 cagagctctt gcatcgggcg ttccatgatc cgctgacggc cttgcccaat cgcagcttgt
     3181 ttgttgatcg actggatact gcgatccgcc aagcgcggcg cacaggggat ctggtcgcgg
     3241 tgttcttttt ggacctggat ggtttcaagg aaatcaacga cgcctacggg catgaaaccg
     3301 gcgaccgctt tttgcagggg gtggcgcatc ggctctcgaa ctcgctgcgc gatggcgaca
     3361 cggtggcgcg ctttggcggc gatgagttca caatcctttt gccatcgctc cagacggctg
     3421 aagatgcgct gcgggtggcg gagcgtgtgc tcgaagaact gcaaaggccg atgcagatcg
     3481 atcgcgtgtc gctcaaagcc gcagcgagca tgggcatcag cctgtatccc cacgacggac
     3541 aagaatcggc gacgctgctg cgcaacgccg accaggcgat gtatcgggtc aagtcgaacg
     3601 gacgcaacgg ctgccagttt ttccgtaccg aaatgaacct gacgctggtg cggcggcctc
     3661 cgcgggaaga agcgcgggtg agcgcgctga cggcgggtgt gctgcggctg cgctatcgtc
     3721 cactggtcga actggccagt gaacaggtgg tcggctggat ggcggaactg tatcggcaga
     3781 ttgccggtac gcgccccgaa gactgtccga gcgaagcatc actgggcgat gaactggcgc
     3841 agcagatttt ccaagggctt gtcgagcagg gctgtcgcga gctgtcgacg cttgctgccg
     3901 gtcgcagcga tgcgccgctg gtggtgcgga tcgatggccg tcatctggtc ggcaatgcgg
     3961 tgctgctgtc agtgactggt gcgctggaaa aaacggggct ggccgctcat ctgctcgagc
     4021 tggatgtcct ggcgccgaac tctttggtcg atggtgcggc gctgacggaa tcgctgtggc
     4081 agctgcatcg cctgggggtt cggctcagcc tgtcggcatc tccgctgtct gcgatgccgc
     4141 tgctgcagct ttcccagttg ccgctgacgg ctttgaccct gcccgaaccg ctgtggcggc
     4201 gcagcatgga agacaaacgg acggcgtcgt tctgtgaggg gcttttggcc atcgccgatc
     4261 gactgggctt tctggtctca gcgaccggca ttcggaccca ggccgagctt cggcatgtca
     4321 agcagctcgg ctgtcagcgc ggccaggggt cgctgtggac gctcggtagc cagccgccca
     4381 agcgagaaga ttcagcgtag ggaacggtgc ttactggttt acgtcgcccg gcgcagccag
     4441 gaacagggct ttttgggccg attcgccgcg ggccgatttt tcggcggccc acttctctag
     4501 ctgggtgcgt gcttcccggc ggatgccaag gtcgggatca aactgcgcaa cgtggccaag
     4561 cagcggcgcg gtttcagcga ggccaagccg gcgcagcgcc acaaccccgg catgacgggc
     4621 gcgtggatcg ggcctatgca agaccgcttc gagcgcaggc agggcggctc gaccgtgcgc
     4681 aagcagcgtc cgcagcgcct gctcttggac ttcgccgctc gatgtggtga gcagctcgac
     4741 gctggccttg aggtcggcgg gctgttcgag acaagccaca agcaaagtcg acagcgccag
     4801 tcccagccag cgtgacgccg tgcgccaaag tggaccgttt gccatcagcc ggtgatcccg
     4861 agcttttgcc gataccacgc gatggtggcg cccaggcctt cttcgaggtc gaagcgcggt
     4921 tcccacttga gcagctcgcg ggctttcccg accgatggca cgcgcagctc gacatcgggg
     4981 tggtcccagc ggacaaactc aatcggcgat gaggacttgc acagctcttt gacgagcttg
     5041 gccaggtgat agaccgtcac ggtagatcgt ggattgccga tgttaaacgc ctggccgctc
     5101 gcctcgggga cactgagcgt aagcagcgtg gcgtcgatga tgtcgtcgat ataacaccag
     5161 gcgcggattt gggagccgtc gttgtggata tgcagtggct cgcctttgag cgcccgaacg
     5221 atgaagtggt gcacggctcc ttcgccgatt tgccggggtc cgtagatgtt aaacggacgg
     5281 acgctgaccg ccggaaagcc aaactgacgg tgataggtca tcgccaggtg ttcggtcgcc
     5341 agcttcgaca ccgcgtaggt ccagcgggct tcgccaactg cgccgacggt ggtggcatct
     5401 agctcgctga cttgataggc gtaacggcca aagacttcgc tggtggaaaa gtcgaggaag
     5461 cggcgacagc gaccccctgc atgggccgca tcaagcaaaa acgccgtacc gagcagtgac
     5521 acccgcatcg tctgggtcgg catccgcatg acggtatcaa caccggcaat cgacgccagg
     5581 tggatcacca cgtcggtgtc tttgacggcc ttctgcaccg cgaccggatc aaggacatcg
     5641 ccaacgacaa gctcgacgtt tttgtggtca tgaaggccgg ctggtcgcag cgcatcgcgg
     5701 cgcatgatgt cgagcacccg gacgcggttg tgctggcaga gccgttcgac aagatgagag
     5761 ccgataaagc cggcgccgcc cgtgatgagt acatgtgcgt cgcgcagctc aacatttgcc
     5821 atggacatgg ccgcgcatgc tatccgggtt ttgctacgct ttgccggtct ttttgttgtc
     5881 ttgtgaaccc gcgccgtccg gctgaccacg cagctgcgca agcctttcgg ccagggctcg
     5941 ttccagaccg ctttggctcg gtgcgtaaaa gtgctggccg gtcagcgcat cgggcaggta
     6001 gtgctcggcg acgtgatggc cggcgtagcc gtgcgggtac ttgtagtctt tgccgtaacc
     6061 aagttccttc gtcagcttgg tcggtgcgtt gcgcaggtgc agcggcaccg gtagcgcgcc
     6121 gtgctgctcg actgcccgct tggcggcctg ataggtcgca atcgcactgt ttgacttggg
     6181 ggcgagcgcc aggtaaatcg ccgcttcgct catcggcagg gtgccttcgg gcaggccaat
     6241 aaagcgcact gcttcagtcg cggcgacggc gacttgcagc gcccgcggat ccgcgacccc
     6301 gatgtcttcg gcggcgaaaa tcaccatccg gcgcagcaca aacagcggat cttcccccgc
     6361 ttccagcatg cgggccatcc agtaaaccgc tgcgtcagga tcggatccgc gcaggctctt
     6421 gatgaacgcc gacacgacgt tgtagtgctc gtcgccggtc ttgtcgtaaa gcagcacctt
     6481 gcgctgcagc gcctccgcga tgctctggcg gtctatctgg cgccggccct gctcatcgac
     6541 ccggctggcg tggagtgcgg cgacttcaag caccccgagc gcccggcggc catcgcctcc
     6601 actgtgctcg gccagaaagt cgcgcgcttc ctcggtcaga cacagcggct cactgcccag
     6661 gccgcgttcg cggtcgctta tcgcccgatc gaggagctgg ccaagctgga cttgtgtaag
     6721 agcaaaaagc tggacgacgc gacagcggga cagaagcgcg gcattgacct cgaacgaggg
     6781 attttccgtc gtcgcgccga tcagaacgat ggtgccgtgt tcgacatgcg gcaacagtgc
     6841 atcttgctgg gctttgttaa agcggtggat ttcatcgagg aacagcaccg tcttgccacc
     6901 atgcgagcgc tcgtagtcgg cttccgcaat ggcggcgcga acttctttga caccggcagt
     6961 gacggcggac agggctgaaa agcgtgcgcc gaccgcgccg gccaaaatgc gcgccagcgt
     7021 ggtctttccc gtgcccggcg gaccccacaa gacaagcgaa gggagctgct tggaccggat
     7081 cgcctgccgc aagagcttgc cttccccaag caggtggtct tgcccgataa actcttcgat
     7141 ggttctgggt cgcagccgct cggcgagcgg ggtgccggga tcgactttgg ctgcgtgttc
     7201 tacgacgggc ttgggctttt caagacggga aaacaagtcc acggcgcgac gctagtacaa
     7261 agccaagggg cgcgtctatc tgtccgcatc gtggggatta cttcaggttc tggagcagct
     7321 tgaccagcca gttgaactcc ggaagctggg tcgacaaaag cgacagaagc ggaatcagca
     7381 cgtagtagac aacgggacgc agaaagctcg tgtcccagct gagcttgccg ggctcggttc
     7441 cggttagctg gctcaggatc gggctgcggt tgagctgcaa cagcaccatc gcggtcagtc
     7501 cacagaccga caaaaagatc agccagatca ggatcgtcat ccactgcatc ggttggaacg
     7561 gatagctcca cagcgcggcc agcatcagga ccatgcacga cgtcgttacc agcatcgagt
     7621 tgcgcaggtg cggcaaaagc cgtcccaaaa tgagcacaag tcgcgtcgac aacagatcct
     7681 cggcgagcgg caagaacgcg agcggatcgg gcgcgggcgg ttccttggcc ccggcatcgg
     7741 gcggcgcagt cggggctggg cgtggtcgca gtggcggacg ggtgccataa agaagcgcgc
     7801 tcgcggcccg ttccaaatcg acatcgacca gggacatggg atcgggcggg ggatttgtgt
     7861 tgtgccacgt cgccacgcgg tcctcgagga ggctcagtga ctcgtgcagt tcgacgggaa
     7921 tggcttgcct gcgggcctgc ggggtctgct gggccagccc ggctgccagg gcatcaagct
     7981 tctgttccag catctgctgt tccgtcgcac cggacagctc ttccaaaagc atggtgccaa
     8041 gcggtctgcg ccacgctgcc ggaatgcgcg aaagcgccga cgccaaggga tggtggcgca
     8101 agtggtggag cgctccttcg agcggccaca gcaaaagcag aagccggaac acggccagta
     8161 caatcagcga tgtcgcgacg ctaaacagca cccgaaacag caggtgcacg gcggacatgt
     8221 cgatggtggt gagcctgcca aacacgggac ttgcaaccag cacgaccagc agcaccaccg
     8281 acaccaccgt caagcgatga tgggaaccga gctgcgcata acacagccgc atgtcttgct
     8341 ggatttgctg tgccttgtcc gggggcgagt acaccggcaa atcggacagc gggcgcaaag
     8401 tgacaagcga aatccgtcgc aggttgtaaa gcccccacaa aaacgaaatc gcaagcaggc
     8461 acaaaagcgg caaaagcggc gacagctcat acgaggtctg ggccgaacga cgcaggaaca
     8521 ggatggcgtt ggcgccgtgc cagcttgtca ggtctttggt atcgagtgcg ctgacggtgt
     8581 ttatggcaat cgacagggcc acaagcgaca gaaacagcgt tgccgccgaa agaaaaccaa
     8641 ggctcgtcaa aaagcgccgc agcctgggcc agcgcggcgg gcgattccac attcggcaaa
     8701 ggacgcagat cgtcaccacc cacgacacgc tctggcttgt cgtcgacatc agcagataga
     8761 gcaggtcgag gatcttgctc cagtcggtgg tgctatcgaa cagcgcattg ccaaaaaagc
     8821 gaccgagcag cccagtgcca tcctgcatga agttcccggc acggaagcgc agcgccaaaa
     8881 ggagcccaag gtaaaacgac agcacgccaa gcggcagcga gagccacagc agatacaccg
     8941 agcgcgcggt gtgttctttg gcaaacagcg ggtgcggact ttgcggcagc ggcttcagaa
     9001 aatcgaaaaa gcgtgcttcc tcaccagcct cgctgaccca cagcggtgtt ccgtaggcat
     9061 tgcgcagaaa cggcaccccg agcgaaagcg acaaaagcag cagcacaatg gtcagcaccc
     9121 gcaccgttcc catgtcgtag ggggtgtagc gctgctctga attgggaatt ttggtgatgg
     9181 tgtcgcgcag cggggttttt tcaccggctc gaaacagggc gtggcgacgg tcggcgacaa
     9241 agtcgtcatc acttggggcg ctgacttcca gcggcaccag cgcgcttctg ccgaccgcgg
     9301 aaatccacac cggcggcttg gccaggcaga gcggatcttg cgcggaaggc ctggtgcagc
     9361 gccacagctt ggcgtagctt tcccagcgct caaagggggc aaacggcgcc gcatattcga
     9421 gcaggtgttc tggatgtccc agcaggagca gcgccgcgtt gtagacgcct tgatcggcct
     9481 ggctggcaaa ctggagccgc tgcatcgcgc ccgaatacgg ataggtccac tgctggttgc
     9541 gggtaaacag cggatacgtc gatgcgacga gcatgccgcg ggtgtacgcg agatcctggg
     9601 gatgcaccaa agacagctcg ctttcaaacg tgaacagccg tgcatcgggg cagtttgcgc
     9661 ttatccgctg ggccaaaaat atcttgtcgc tggcgtcggt catcatcagt ccgacgtagc
     9721 gaatgccttc ttgatagagc gtcgatagga gcgctgacag cgaaagatcg cgctcgctca
     9781 gggtctcttt gctaaaaaga ggcaacatgc cggtgcggcg gctgtcgtcg cgctgccgtt
     9841 ccaggctgtt tttggccagc tcttcgtagg tcgccgcgga cggggtctgg gtggtgtttt
     9901 ttttgcgttc ccactcggct tgcaagcggg gcagatccga cggcaccggc agaagccttg
     9961 gcggacaagc gtggctgcgt cggacgcgac cgcggtgcag gctgcgatct tgctggtagg
    10021 tggcgttgcc atagggggtc gatgactcga cgatgagcgc aatcttgcgg cagtcagccc
    10081 cgagccggtc gacaaggaag ctgaacatcg cttcttgcat gtcctcatca gagtgaaccg
    10141 tcgagtggaa gcgcacttgg ggtccgctgc gccgcgcagt gcgcagtgct tgggccacgt
    10201 ctgtcgaagt ggcgcggccc gagatgatct caaggggata tttgcgccac agctcgtggc
    10261 tggcgatgaa gtgggccaga ccggctgcgg acccggaaaa cgcgggtgcc aacagcgcca
    10321 agggttcgtc ttgcgggggc ggaccgtact cttgccgcaa gctgtcccag tcgccgagcg
    10381 cttcgccgag cgccgccaag cgaattcctg ccgtcggcgt ttcttcaatc agatagacga
    10441 cgtaggcgtt ttcgggggaa aacggcttcg ggggttcggc ggctggcaca ggtgcctgct
    10501 tggctccacc tgacggaagc ttgggggtcg cttccttgcg catcattcgc gtcggtggat
    10561 tgtaggcgtc ggtgttttgt ccggtaaaca caaggacgcc ataggccagc ggcgagtctt
    10621 tgccggtgac gcgcggcagg tcttgggcgc gcagcgtata gccgaccgtt tcaaacgatc
    10681 ggaccatcgc ttcgagcatg ccgtcgagtc cccagcttgg ctctggcacg gtcacaaaca
    10741 gcgccgacac cgttccggca tagctgcggc gcggcgggga cagcggatct gggggctgcg
    10801 atccggtctg acatcccggc tcgacgttgc cacccagatc gcacagcagc ttgtgcgacc
    10861 cgcgttgcga aggtgcagcg tcttttttgt cgggggctgc atcgtttttc agcgccgatg
    10921 cgggcgctgg cgctgcgggc tttccctctg cgcctgtgcc aagccctgcg acgatggtgc
    10981 cggggacgac caccgtggcc agcacggcca gcatgattcc gacatagctg ctcgttcgat
    11041 cgccacctgc catgcgctgc gcactccgtt atgtcatcgt gccgcgtttt ggctccttgg
    11101 cggtagggga gtcgacgcgg acttcgcgac ttgtgaagat gccatggcgg cgcagacggt
    11161 cggcaagaga tgacttgggc acgtccagaa ccaatgcggc agtgcgctgg ttgccgccgc
    11221 tgtgccgcag gacttgcgcc agtacgtcgc gttcgatgtc caccaatcga cggcccacaa
    11281 cctgcacttg gctgtcgctt gagctgggca ggggtttttg ctggccgatg atgtcgcggg
    11341 cgcgcagcgt cggtccatgc aagtgggcgg cgcgcagaag cgccatccga agctcgcgga
    11401 cattgcccag ccagggagcc tgcatgatcg cttggatctg atcgtcgtcg atgtgaagcc
    11461 ctggatgctg gaccgcaact tcggtcaaaa agtggagcac gagcagcgga atgtccgtcg
    11521 ggcgctctcg cagcggtggc agatcgataa ccagcccggc cagccggtag tacagatcgg
    11581 cgcggaaccg tccatcggcc acggcttgcg gcaggttgcg atgggtcgcg gcaacaatgc
    11641 gaacgtcgac gctgacttcg cgggtgctgc cgaccgggcg aatcttctgg gtttccaagg
    11701 cgcgcagaag ccgcggctga agctcgatgg gcagctcgcc cacttcatca aggaacaagg
    11761 tgccgcccgc cgcctcggca aagcagccca ggtgcttgcg atcggccccg gtgaaggccc
    11821 cgcgctcgtg tccgaacagc tcgctttcga taagctccct gggcagggca ccgcagttta
    11881 tcggcaccag gggtccgttt cgctggctgg cttcatggat ggctttggcg accagttctt
    11941 tgccggtgcc gctttccccc aggatgagca cgggcagcga cgatggggcc acccgcgcga
    12001 tctgcttgcg cacggccagc atctgggggc ttacgccaac aagtcccatg ctgtgcggct
    12061 gcggtttgcg cgcaagccac agctcggttt caccaagtcg cagcaccccg ccatcgagca
    12121 gctcaccttt ctgcaagcgg accccattta caaacgtccc attgcgcgag ccacagtctt
    12181 gcaccagcac ccggtcttcc gacgcggtga gcagggcatg atgctgcgaa acatagctgt
    12241 cgtgcagcac ccagtcgttg tcttcggcag tgccaagccg cagcttgcgt tccaaaatga
    12301 tctcgatgcc gtcgcgacga cgcaagatcc acggctgatc tggctgcgcg gtgggctttt
    12361 tgatccacag cggggtttgt gcctcggcag tgccgtaggg gacggggggg ccttcgcgca
    12421 gacatggcgg ctcagcggcg gctgtcggcg ccagcgccgg ggcggcaagg tggctcatga
    12481 aggggtccgc gattcgcatg ctcagtcggg tcatggtcat cctggctgtt ttttgggttt
    12541 tcgcctgggt cgtttgggcc caggccaccc tggtgtgaaa ggtggtgccg tgaaatcgtt
    12601 tgtaggactg ggtaggtttc gtgcgcgggt gggaaaaagt tgctaggttt ggggcccttt
    12661 tttcccaaat cgaagccatg tctgacctgc tcatccgtgc cattcaccgg acgctcaacg
    12721 tccgtatcgt cgctgccatc tccaccgagg tctgcaaaga cgccgcccgc cgtcaccgag
    12781 ccacccccgc ttctgcctgt gcgctcgggc ggggactgtt gggcgcgtat cttttggcca
    12841 ccctgacccg cggcggcgag cgcgtcacca tccaaatcca ggcaaatggt ccgctgcgcg
    12901 gcctgaccgc cgatgcgtat gacgatggtt cgatccgtgg ctatccactt gcccctgaag
    12961 catgtcccac cgccaagatg gatcggcggc agcggatggc ggacttgatt gggcgcgatg
    13021 gcgttgtcaa cgtcgtgcgc gacgtcgggc tcaaggaacg ctttcaaggc caggtgtcgc
    13081 ttatcgtcag cgaagtcgac gaagacatcg aatcgtacct gcgccagtcc gagcagatcc
    13141 cgtcggcgct cggaaccgag ctgatttttg acagtggcgg cgcgatcatt caggccggcg
    13201 gcgtgctgat ccagtcgatg cccgactcac ctgacgaagt aaagacggtg attcgtgagg
    13261 cacagcacct tctgcgcgct ggagaacttt acgatttgct ggcggcagga ccgcagacgc
    13321 ccgagtcgct ggccaagctt gtcttgggtc catatgccga cgggctggaa gtgcaagaca
    13381 cgcgcgagct acagttccgc tgccgctgcg atcgagagcg ggtcgcggcg atgctgcgcg
    13441 ggcttggcga aatggagctg gacgagatga tcgccgaggg caaggccgag atcacttgca
    13501 acttctgcgc cgatgtctac caattttccg tcgatgaact gatcgagatc cgtcgcagca
    13561 aacagaaagt cacaaactga tgcgggggct gtcgcacttt ctgtgtgtcg cgcccgaggt
    13621 cgcccatgcg cttgccgagg ggcgagcggt ggtggcgctg gagtcgacgc ttttgtcaca
    13681 cggtctgccg tggccggaca acctggatgt ggcgctcaaa gcggaagcgg cggtacgaca
    13741 gtcgggcgca gtaccagcga cgatagcggt gttgggtggt cgcatccaca tcgggctttc
    13801 aatggcgcag cttgagcagg tggggcaagg gcagggactc tttaaggcat cggcggcgga
    13861 tctgggaccg cttttggccg cgggagtcga tggcgcgacg acagtatcag cgacagtggt
    13921 ggcagcagcg cgggccggga tttcggtgat ggcgacgggg ggaatcggcg gggtacatcg
    13981 cggcgatggc tttgacatct cgtgtgattt gacgacgctg ggcagtgaag cggtggcggt
    14041 ggtgtcggcg ggggccaaag cgatcctcga tctgccaaag acgctggaat atctggaaac
    14101 gctgggcgtg ccggtggtgg gttacggaac cgccgagctg ccagcgtttt acacccgcaa
    14161 aagcggtctg cgccttttgc accgggtcga cgggcccgag cctgcggcac ggctcattga
    14221 ttcgcacttt gcggtgcatt cgcggcgcgg ggtgcttctg tgcaatccga ttcccgatgc
    14281 cgaagcgctg ccgtcagagc ttgtcgatcg ggcgctgcaa gcggcacttt ctgcggcaga
    14341 gacggcgcag gtgggcggca aagcgctgac gccgtttctg ttggcccgga ttgcagcgga
    14401 aacggggctg cgcagcgtgc gggcaaactg tgcgctaatc gtcgacaatg ctcgcgtcgg
    14461 tggggaagtc gcggtggcgc tttcggccca gcggaaggcg gcctaggttt tcgcccggcg
    14521 cgtccggcaa agaaagagtc ggcgctgtgt ctaggaagca gatatttacg gtggcaagcg
    14581 ccgatccgcg cggtctgttg gcgctgttgt ccgatcggct gggactgtcg cttgcggcgg
    14641 cgcagcaagc ggtggtcgaa gggtcggtgt ttgtcgatgg gcgacgggtg aacaaaacgc
    14701 cacccgaggt gttgcccgga caaaaagtcc aggtctttgt gcccgacgag ccgcctgtgc
    14761 ccaaatcgcc gcggattgtg tttgaagatg cggacctttt ggtcgtggac aagccggcgg
    14821 ggcttctgtg tcagccgggg cgacggggtg ggccatcgct tttgacgctt ctgccggggc
    14881 ctttgtggtt gccgcatcga ctggatgccg acacttcggg gctgacgatg ctggcgcggt
    14941 ccgcggcggt gtgtgctcgg cttgggcaag cgctggcagc ggatcaggtg acgcggacgt
    15001 atgcggcgcg ggcttgtggg cttgtgcccg agcgtggtca ggttgcgctg cggattggca
    15061 aagcggcagg tccagcggtg gtcaagatgc gaacctatcc gccgcagtcg acccagggcg
    15121 atccggcgct gacgtcgttt gtgaggcggg cggtgtccga ttccagtcag ggggccgact
    15181 cgcttgtgtg gctgcgactt tcgaccggca gaacccatca ggtgcgcgtg catctggccg
    15241 ggatcggaca tccgatcgtc ggggactcgc tgtacggcgg gcggcaggcg tcgcggctgc
    15301 ttctgcacgc ggtcgagctt tcattttccc atccacggac ggggcagctt gtgaagctgg
    15361 cttcgccgct gccggcagag ttttggccgc tgccagtcga cgatgcgctt ccgccacact
    15421 ttggctgacg cgggttagcg cggcgtcacg ggcggggcga tcagctcgac ttcgttgtcg
    15481 ccgtctggca ggtgttcgtc gaccgcggtg ccggtgcgat cggtatagcc gacgcggttt
    15541 atgccgtcgc ccatcaccag tcgatagggc aaaaagctgc gtggcttgcc gtccagatcg
    15601 aactggtagg tgatgatcca gtgcttgcgg ctcaagtgtc cgtgggcacc ctggccgagc
    15661 agtgcggctt tggcgttctg ccggacggcg cggtcttcgt cgctggcgct ggcgctgcgg
    15721 agccgggact cgacggttcc ggctcggggc acgagcaagc cggcgagcag ggccgcattg
    15781 cgccgaatgc cggggtcggg atcgtcgagc cactgcagat cgctcgtttc ggcggccccc
    15841 agacgggcca gcgcaccgag gacattgcag cgcagcgcgg tcaaatcggg cgatgactca
    15901 cggcggattt gtagcatcgc gcgcagccgc tcgacgacac ggccacaggc tggatggttg
    15961 cggggaaggt tggacagcgc ccacgcggtt tccgccaacg tgcgcaggtc gccgccatcg
    16021 gaaagctggg cgacaagggc gctgattgcg ttttcggtcg gagttgccga aagcagcacg
    16081 ccgagcgccg cggtggctcg gttcacccgc agcggatcgc catgggacaa aagccttatg
    16141 agtcggctct gggcgggcag atcgacaaac gactggatcg ctgccagcgc gtcgacggca
    16201 agttgtcctt ccgcagagtg cggctcggca cccagttgcg acagcgcaag cagtcgttcg
    16261 cgcacggtct cgctgccatg gccgcgcaaa agtcccgaca acagcagcag cacttcactg
    16321 tggggtccac cgtcgggcgg cttgcgcagc gcttgcaaca gctccgcgac aatcggtcct
    16381 tgcttttcgg ggtgagggcc aagctggctg gcaagcgctt gggcgctgcg gcgacggatg
    16441 cgtcgctcgt cttcaccggg acgcatcgtc agcacggcgc ggaccaggat ggacacactc
    16501 ttttcgtcaa gagcgccgct gctggccagc gcctgcatcg caagcgccgc cgcgagccgg
    16561 gtctctgcgt cgggaagcgc gggtcggtcg gtcgggtcga gcacttcgca gagccgcgcc
    16621 tgtccttgct ggagatcacc cagcgcgagc agagcttgac gctgaacgtg tgggtcggcg
    16681 tcgctggtgg cggcgagcag tgcgtctttg gcgcggacat cgagcaggtt gggttttcgc
    16741 aaaagctgca cggcccgccg tcgaacgatc acgtctttgt cggtcaaaag cgatgcaaaa
    16801 agtgccgcgg tgttgcgatc cccaatggcg gccagggcat cgaggagcag ctctttggga
    16861 acccggtttt gggacagctc acgcgagagc acctgttcgg cggtgcgacg atcggggggc
    16921 ggcagcttgt ttcccaggtc gcgcacaagc tcgatcagcg tcgcgagctg cgccaagtcg
    16981 agcgccggac gcgccagctc ttgcaacagg atcggcccga cgcgatcccc ggcggcgacc
    17041 agggcttcga cgacaagggg tcgcagcgcc ggattgccaa ggctcgtcag gatcgcttgg
    17101 gtggccaagg ccagtacgtc ggcggaaagc cgcttgggcg atgtcggcag caggtaaaac
    17161 gaaagcgctt ggacggcgga agcgcgctgg ctgtcactgc cggtttcgag cgtctcacgc
    17221 agaagcggaa tcgccgattc tgccgacagc cgtcccagac agagcatcgc ttgcttgcgc
    17281 acttcgtcac tgggatcgcg cagaagccgc agcgccgcgg gaatggcact gtcggcgcgc
    17341 aaaagaccca ggctgcgcag cgattcggcg cggacatcgc gggctggatc ggacaaacga
    17401 ccgagcagtg acaagagcac ccgcccgcgc tgatccgcag acagcgaaag cggactgagt
    17461 cggccaagca ccgccattgc acagcggcgg acactgacgt tttcgtcttc ggcccgcagt
    17521 agcaaaggcc ccatcagcgg cggcagatcg gcgacggtga tgacctgcga aaacaatagc
    17581 cgctcgacgg cccgcagcgc ttcttggcgg acttcgaact gaccgtctcc catggcgcgg
    17641 gcgagcaggg caatggcgcg ctgctgctcg gctttgggaa gggcaccata ctgcccaatc
    17701 gcttccgcac tggcggcccg aacggtgctg tcggcgtcgc tcatcccggc gagcaaggga
    17761 ccgattgcgg ccaagctacc gatcggaccc agcgcttgtg ccgcgcgcgc ccgcaccatc
    17821 ggctcgccgt catgcagtcg ctgtacaaga tacggggagg acagccgctg gggcagcgct
    17881 tgcagtcgct cgattccctc tttgcggcgc tgcggatcgc tggcttgcaa aagggtgcgt
    17941 acctcgtcgg ggaggtcact ctgtccacca tacagatcgg ctgccaaaac cgagccgcta
    18001 agcagcatcg acaacggcag acccagaagc cagcggcgca tacgcacagc ttgaggttat
    18061 accaggatgc ggcgtcgctg cgcactccgt gtgcggtttt ctgggtttag tccacgcggg
    18121 cgatgcagtg acccagcgct tctttgagcg tgcgcgtcac cgacagttca cccaggtcga
    18181 ccccaaggct tgtgactgtg cgaacgacct gcggacgcaa gccacagagc agggcggttg
    18241 cgccgagcag accgaccgct tgcgcgacct tgagcagccg tcctgcgctt tctgcatcga
    18301 gttcagaaat gcccgtcagg tcgagaatcg caaagcgcgc tttgtgctgc tggagggacg
    18361 cgagcaggtt ttcggtcagg gtttgcagcc gcgacgcatc gaggtgtccg atgagcggca
    18421 ggaccagcac ggcatcccac acctggatga tgggagtcga cagcgccacg atcgattcct
    18481 gctgactttc gagcatgcgc aggtaggact gtagctgggc atcggcggcc tgcgcgatgg
    18541 catgctgggt gcgttctagt ttgctgacgc gctcgtcgga acggaggatg tcttgggtga
    18601 gttccttttg cgcatgttca agctggcgaa tccgattctg tttgtgtcgc agcaggcggg
    18661 cggattcctt gatccgctga agctgtttgg acaagaccgt ggcaggaatc ggtgacgcgc
    18721 cttgcagaag cggacaggaa tcacagccgc ttagtttgcg cgcaaacgca tcggcatcca
    18781 gtgcatcgca agccgggtct tccaagcagc agcgttcaca gcgcataaac agactcgctt
    18841 aggagtgctt gccaaaccgg attgcagcga cggtaaacgt cgattcgtga tgctgcgtca
    18901 cttcggccac aggggacagc tctccgtagg tgtaaaaacc gatgcggggg acttcctttg
    18961 gcaaaacgga atcggccacc acgtattcct ctttgtagcg cggcccgaga atgattttgc
    19021 gtgacatgca gttaaacaga agcatcagat ccgcgtcttg gcaggacaga agcgcggact
    19081 gcatggcctg ttttgtgcca cgcagaacat cgtcacgggt tgcgctcaga atgcgcagct
    19141 cggccccttc cggaatgtca cccccaagta caatcgcacc ccggttttca tccatccgga
    19201 atgcggcgcg caccacatcg accgtgacgg ttttcgcatc cgccgggcca gacagaacac
    19261 cgatgggata ctcgatcgtg accgcaggaa gttcgctttt gcggggtcca agaaagtcca
    19321 gatacacgtc gagcgcgggc ttcccattga gttcgagcac cacgtttccc tgttcgactt
    19381 tggttgcacg aagcggaatc gaaaccgggg tgtatccact gaaggccgct gcggaaatgg
    19441 aaatcggtcc atacagcgcg agcgcgaccg caccacttgt ctgtagattt gatcctgcga
    19501 tcatcgtggt ctgtttaaat gcgcccatgt ccgcaggtgc tccaccgacg attggcgtgc
    19561 ggtctccgat caccttctgg atgccgcgca gaagccgcgt ggcattgagc gaaaggacat
    19621 ccggaagcag aatcagcaga tcaggaacct gtccggaaag cggaaaaaca agctgcttcc
    19681 caagttcata ggagtcgctt ccacccgcaa cagagagcac gctggcgcga taccccggaa
    19741 gtaccaggcc aagcgcagtg accgagtggg tgagcgctcc atcaggaccg atttcagcaa
    19801 aggaagaaca gcccaagacc tgcgtggtag aaggaagctc gctccacaga ccgttgcgaa
    19861 tttctgcggc agcgaactcc gcagaaaaaa acaccaccac cacatcgggg gtacagccaa
    19921 gtctgtcctt gagctcaaac gccgcttcct tggcggcttc gtgggcgtcg tctcccgcga
    19981 tcgacaccgt tgcaacatga ctaccggacc acaccatctt ggactccttg ggggccctgt
    20041 aggaacaaag acacagggca ccaaagtcat cgtaccacgg cgctaggtgc gtgaaaagac
    20101 gtgggttttt tgggtgcgga agtgtggtta ggggccggtt gcgtagaagt aatttaggca
    20161 catctcgtcc gtcgtcgatt caccccagcc gactggactt gcggtgttgt tatcgtagac
    20221 gcacgtaagc ttgatctggg tgcctttggg aaacggcaga ccctggcttc catcgtagaa
    20281 atagaactgc tgccagttga agtcccagct tggaatgtcg ataaggcaag tgtcagcggt
    20341 ttccacgcgc agtcgtttgc caagctggtg cgcatgcggg gccagaccat agataagtcc
    20401 gtccgatggt gtcgtcattg ttcccaccac ggcgtagtct gtcgcgccgg acggaatcga
    20461 aaacgacgca ttgcgaaccg acagcacggt tgcttttttg gccaccggcg tcttggcaaa
    20521 ctggatcttt acgctggtct gatcggggct ccatgcgccg ccgtggtggt tgtcgtagtg
    20581 aatctgcatc accagcactt ggccggcagc cagcgtgatt ccggtgttgg ttggatactt
    20641 ggttgggggg cttccgggga cccagccgcc gatggttgat gcggtcgaaa ttcctggtcc
    20701 cccatagcat ggccagccga cttccggagt cttggcatct tgcgcactcg ccgtcgctgc
    20761 gtccgcagga tagagcagca catggtgcac attctgtttg gcccccggtg tcacttcata
    20821 gccaatcaca tcgcggcttg cggcaagctt tggatcaagg acaaagcagt gataatcggt
    20881 ggtcaggttg gccgccgggg tataggaagc cgcaggggac accgttgcat cgacccaatc
    20941 gagcgtcacc acattgggag tcatgttggg cggcgcatcg gcaggattcc cagccggcga
    21001 tccatcggcg acccaggaat aaaccgtgtc gatggcgtct tggccgagcg agcgcgcgtt
    21061 ctgaaacttt tggcagttgt ccgcgggttt ccacggcggc atccttcgcg cgaccaccgc
    21121 ggaagagacc tgcgccgcgt tttgcagcgc ttcatcgtaa gtggtaagcg gaaagggtcc
    21181 gactccgccg accacatggc acccttgaca gctacgctgc atgatcggct ggatgtcttt
    21241 gtagtacgtc atgccggttt ttttcgtcac acccggcggt ggtggaaccg gcggcagggt
    21301 gttggtatga ctgtcgccac aaccggacaa aagagcgggg acagagacag ccagagaagc
    21361 agccagagaa gcagccagag aaacagacag agagacagac tgagagacag accaagcggt
    21421 ggaaacgtgc tgcgagcgcg ggcgcggcga acctggagag atgcggatca tcggatggga
    21481 ctcctacaga tgtggttggc aaggcacggg tccgcagcga aagcgcgagt gtctgtcgtt
    21541 tcctgttacg gaatttgatg gagccaccca gccatcggtc ctccaatgaa ggctgagttt
    21601 ccggaaggaa acacggtccc cgcttgcagc cgctgcgccg tgtccgtctt gacgaccgaa
    21661 aacgaacttc cgttccattt taggaccgtc ccgccatcac cgacgacgta catgtcggaa
    21721 tggtttttgc caaaaatgtg tcggaggtgc tgggtggtta ccgtgtaggg ggtccgcgag
    21781 ttggtgccag tttggtgacg gaagagggtg ccatttgatc cggcgaccca gatgtcttgg
    21841 cccatgccgc tggcgctcgg ggtgatccag atcgcgtagc cagtctgtcc ggtgatcggt
    21901 gggaagttgg cgttggcgat gcatgtgctg gcacagtcgt agacgcgggc gtctttgccg
    21961 acggccatga cgttgattcc cgcacaggcg acgtcataca agggagagcc tgtgatgatg
    22021 ccgccagcgg tccaggacga tccgttaaag cccgtcatct gaccgttggc ttcggctgca
    22081 tcgtgtccca ctgcaacgac ctggtttgcg ctggcgccgc agatcccgta caggtccatg
    22141 ctggtgccgc tgctggccgc cgtccagatg ttgctggtgt aggaatacac gccgccgccg
    22201 ctgccgaccg cccacgccgt ggtcgttccg gtcggtgctg ggtccaccca cacggcattg
    22261 ccgacaaaac cggcgccccc caatccgact actttgctgg tgctgacaaa gtcggtggtg
    22321 agcgcgtagg tcttcccatc gctgcccgcg ccgattgcga agacggtgct cggggtggtt
    22381 tggctgcttg cgccgagcga gcggaccacc gggaggattt cgtcgagcga gtcaaaccac
    22441 agcgttccgt tccatttgct acgcaccgcg cccgtgctgc cgccgattgc ccaggccgca
    22501 ttcgacgaaa acgcctggac atcggtgttc atgcgcttgg aaggtggctc cgtcggcgaa
    22561 agcaatgaaa agccgcccga tcgacgcagt gtcaccttgt cgcccacgac aaagcctgtg
    22621 ccatcgccag caagtgatcc cgcgacaagc caagcggtgg tgccgctcgg cacggctgtt
    22681 gccgtcgaag acgtaagctg aaacagcgca cccgaaggac cggatgcaaa cacattgctc
    22741 gcattgctgc caaacaccga atccaggttg gtcgtgatgc cggtcggaag cggtgtccat
    22801 gcggtgccgt tccaatatcg cgcggtgccc gactcaccaa ccacccacag attctgttca
    22861 tcggggcccc agatcgaact gagcgccgcg gtgccgccgg ggctttggtc aatccacttg
    22921 gtgccgtccc acttttgcaa aaatcctgcg ctgccagcga cgaaaatctt gccgcttatt
    22981 ccataaatgg cgcgcagact tacgcccgct gggatggtga tcgggacttc gctgagggtc
    23041 tggctgagca agcggtagac cctgcggtct tcgatgaacc agacatcgct tggcgacacg
    23101 gaaaaaacgt cgacaaagcc ggttgtcgcg gcgcacgtcg tgaccgggtt ccagtacgtg
    23161 ccgttataac gagcgaggcg ggtttggctg atcgcccaga tgtcactttc gctggtcccg
    23221 tgcagccgct tgagcggtcc catcgagagc ggcgcttggg tacaaaacat gccgctgccg
    23281 ctgccgctgg tgcacgcgaa cgcgaccgcg cctgtgcagc tcgacggcgt tccgccatcg
    23341 ctgccgctgc tcatcgcgag atcgagcatc gtggtggcaa tcggttcgca ctggcgggtc
    23401 tgcgggttac agacctgatc ggatggacag ccgtagccgg gagcttcgca gctgagggga
    23461 ttgggcgtct tatagccgat ccataggctc tcgcagccga cgagcagtgc agtggacaaa
    23521 agcagtgttc cgcagagctt cacgacgtgg cgcatggttt cccccccaac caaacagctt
    23581 actttgtcgg ccaggcgatc atcagcgcgc cgccggcagc aaggcccagt ccggccccga
    23641 gcagtccacc gcccacgctg ccggtgttgt agaggtagtt gcacggggcg cccgcaggcg
    23701 gggccgtcgt atcgccgcat gttccgtttt gggacagcgc gttggccccg aagccagaca
    23761 gcaaaaaccc cgaaacgacg agccctccac cgacgagata tcgccacaag ggacgcggct
    23821 ggggcggctg attggtctgc ggtgccagtg gcgtgccggt tcccgcagta agcgttgccg
    23881 cgatctgccg agtctgtcct tgcaacagct caacgggttc gtcgtagtcg gaatagccgg
    23941 gcagccgcag cttcagattg tggtgaccga cgagcagcgc ccgctggtac ggcgtctcac
    24001 cgcggactga cccgtcgatg accacctgag cccctggcgg cgtggagctt acgtcgagca
    24061 cgccacgcgg atggttttgg gcatctcgca aaagctctcg tgacagcagc gctgccgctt
    24121 cccgcatcgc tttttcttgg cagtttccac acgcgcgttc acgctgcgca gcgacgccac
    24181 caccgtctac atcgaaccac tcggcgcgaa tttttccggc ggcctggtcg acgtgaactc
    24241 gcaccactgc gcgcacaccc aagtcccgcg ccacgctctc ttggcaggct gggtcgcgca
    24301 ggcagtcggc aggtcgggat tttttcgcgc gctccagtcg ctgcgtggac aaaagtgtcg
    24361 cgcgctcgcg cttggccagg ctagacacgc tctgttcgac gatgctgcgc tgacctgggt
    24421 ccatgtccgc cggctcgatc aaaagaagcc actcgctcga cagactgagg atcgcgaccg
    24481 cggttccgcc cgcacccggc gtcaggtgaa gctgggcccg caaagacggc agcagatcga
    24541 gcgggtccga ccgataggcg ctgtttttta tgatcaagcg gaagcggtgg ctggttggca
    24601 aaagcagcat cggccctgac agcggcagca tgccgaccaa gtgatcgtcg acgtacagca
    24661 gactgccggg cggcccaaga acatcgactt ctgcgacggg ctcgtgcagc gcagaaatca
    24721 gcccggttag ccttgttttc gtcgtttcat cgaccgattc gcccatttca tcgagatagc
    24781 gccgatacag atccgccgcc gccgcggtcc gtccttcttg ctctgccagc ttgccaagct
    24841 ggagcgtgat cgcgggctgg ttttcgatcc gcagcgcttg ctcgagcagc ttttccgcct
    24901 gggcagggtc gtgcgcactc cgggcctgtt cggcttgcgc aatcaggtcg cggacgcggg
    24961 tgcgcgcatc gatctgcgca cgggccggtt gggccgcacc cacaagcagc caagcggcaa
    25021 acagacgcga cgcgacgtag gggaggtttg acatggcacg gcccttttgc gagccaactt
    25081 cattttccta tggcagcgcg gcggtcggca atcgctaggt ttggcttgtg ctacctttgc
    25141 cggcgatgga accgctggct gggctgcgaa ccgaagcaca gatacatggc tttccgacgg
    25201 tgattgacct gccgaaagtg cgcgttgcgg tcgtcgaagg accggatgcc ggcaaacact
    25261 tcgacagtga cgacgagatg ctggtgcgga tcggcacccg tgaaaacaac gggctggtgc
    25321 tgtcggacaa aagtgtcagc ggctaccaca tggagatcac gggcacccaa tcgggctggc
    25381 gtctgcgcga cgtcggcagc acaaacggta catttgtggc cggcatgcgg gtgttcgatc
    25441 tggtgctgcg atcgggaacc gtggtggcgc tggggcgaag caagctgcgg gtcgatgcga
    25501 tccacgacga gcccgtgcgg gcggcgctgc atccggcggg acgttttggc gacatggtgg
    25561 gccagagcgt ggtgatgcgg cggctgtttt cgcggattgc gcaagtggcg cgtgccaact
    25621 cgacggtgct gatcacgggg gagacgggga cgggcaaaga agcggtcgcc gaggcgctgg
    25681 tgcaagcgtc actgcgcgcg agtgcgccgt ttgtggtcat tgattgttcg gcgctaccgc
    25741 acaatctgat cgaaagtgag ctgtttggtc acacaaaagg tgcgtttacc ggcgcggtca
    25801 gcgactatcg cggggcgttt gagcgcgccg acggcgggac gatctttctc gatgaaattg
    25861 gcgagctgcc gctcgatctg cagccgaagc ttctgcgggt gctcgagcga cgtgaagtgc
    25921 gacggattgg cgccgagcgg acgcgatcga ccgatgtgcg ggtgattgcg gcgacgaacc
    25981 gagtgctcgc aaccgaagtc aatgaacgtc gtttccgtca ggatctctac tatcggctgt
    26041 cggtggtaaa cgttcacatt ccgccgctgc gcgatcgccg tgaagacata ccgctgctca
    26101 tcgcgcattt ttacgatcgg ctcgccgatg ctcatcgtgg tccatcgctc ggcgaagtca
    26161 cccagcggct gtgcgacaac gactacacct ggcctggcaa cgtccgagag ctgcgcaatg
    26221 ccgtcgagcg ggcgtttcat gtgcccgacg aagatccgtt ttttgccggt ggcacgccgc
    26281 tgcgagccga tgcgtcgtca gaagcggtcc acgtcgatct ggcggtgccg tttaagaccg
    26341 ccaagcagaa gctgattgat gcgtttgaaa aggaatatct gcaagcgatg ctcaaagcga
    26401 cgcgcggcaa catttccgag gcagcacgac gcgcgcagat cgatcggatg catctttaca
    26461 agctgatcac ccagcatcac ctaaacggcg agtaggtccg tcaagcgatg tcgtcggtga
    26521 cgaaggccgg cgatgaaggc cggcaagggg tcgctacttt tcgtagggca gggggctttg
    26581 gccgtcccaa agcgacgcgg ggattttgct gccgcgttcg atgcgttcgg cgtcgctgca
    26641 gcggtttaaa aggttcggtt ccgtctgcgg tgtgcccata aagcacgggc cttccgccag
    26701 atcgcctttt tgcacgccgc catcgccagt caacgccggg tcgtcgtctt cgatgaagtt
    26761 gttggcgtcg gcgcagcgaa gctggctggc aaacagcaag agcgtcccga taagcagcgc
    26821 accaccgatt cgcggggata ggttttgcac aagcctacgc ggcatcactg ggcctcacag
    26881 tatttgcgtc ggcagacacc gcttgagcag gttccggtgg gacactgctg gcagctgagt
    26941 gcgccttggc tgacgttgtg ctcgaagaag cagtggcagg gttcgccggg ggcgtacacg
    27001 gtcatcggcg cgccatcgct ggtcttggtg acgcgcatcg cgcactgcgg aatgaaaccg
    27061 ctttggacct gcaaaagctg cgagtcttga ccaccaacct gcgttttgcc gaggaagatg
    27121 ttagccagac gcgcgccttt tttcgacagc ggctggccgg ggtttccggg gtcttgccgc
    27181 aagatcgtgt gaagaaaacc ccacagcgga taatgtccgt cgcgcacgct ctgtttgtca
    27241 tacgtggtcg atcggcggtc ggggaaaaac gcgccttttt gaccaatggc ctggaacccg
    27301 agcgggcgca gctcggcgcg acggcgatcg gcaaccgtgg tgggcacgat gccgatgcct
    27361 tgggtaccgt cgttgtagat gatgttgatc agatcgtcga tcgagccggc ggttgtgcct
    27421 ttccagcgac tgttcgccag cttgatccgg ccaccgacga gcagcgtgcc tgcgtcctgg
    27481 gtgcccaagt ggccaatcgc cgcgtcgttc atccacggct tgatcttggc ggcagcaccg
    27541 gcaccaaaaa caaagtgggc ttcttcggcc tggatcgctg attccgtcgc cgatcggagc
    27601 atcgccagcg agtacgacga tacaggcccc gccgtatcga tgattcccgt cggtgccgtt
    27661 tgcgtgtagc tcggacagct ccgcggaaag acatccgaca gcacgatgtc aatcttgttg
    27721 ccggtggtgt cgaggtcgca ctcttttggc gcggtgtcgc gtgggtccag cgtaaagaac
    27781 atcgccttgc cgcgcatgca tgcatccgac gcgcacgatc cgggcaaggt gtcttttacg
    27841 agcgactcca gcgccgtgca gcttgggcgg atctgataga tgacggtggt tcgctgcgca
    27901 tcggtgcgct ggagcaggct ggccactttt tcgaccagcg gctccgcaga gggcgtcgcg
    27961 gtcagatagg tgggttcggg cagcgtactg cactggagcg cgaaggcccg tcccggcaga
    28021 gccgcagcgg cgacgaacaa gagcacggag aggtgcctga ccgacatgtc gcgcagccta
    28081 gcacaagggc gacaagttca agcagttttc acggactcaa ctgtacggaa ggtccggtgc
    28141 gtgggcccaa tgtagctgcc ggggatgaca agcggtgtgc agactcgtcg acaaaaccag
    28201 gaaggtcgcc agtgcgcaac agcggctcgc ggacggtcga taaccatggt cgagacagtg
    28261 ggtaactttg cctgtgaccc gtttccgaag aaaaaagctc caagtcggct ctccttcgtc
    28321 gatggcgcag gcgtgataag ctgcaaacga ggcaaataga agatggcggc ttccagcaag
    28381 acccggccct atatttccag ccgttttgtg ctcgaactcg acgattctgg ctcgggccag
    28441 tacatgggca cgctccagtc catcgagggc ggaaccttca agtccgaagt tctcgaagag
    28501 aaagtcggcg gtagcggcgc ggtgaccaag tatccgggtc ggcccaagtt cgaggacgtg
    28561 acgatccaag tcggtatgac gatggcgccg cggttttggc gctggctggc aaagagcttc
    28621 acgtatgacg cccagcgcgt cgacggatcg ctcgtcgcac tcgactttga caaccgcgaa
    28681 agatggcgtc gtcgcttcag cggggcgttg attcgcgaag tcaccatccc aacgctcgac
    28741 ggtgcggcca aagaacccgc ttatctgacc gtcaagtttg ccgtcgagac gatcaaagaa
    28801 gagcagtcgg gaaacaacaa atatcccggc gagcagcagt acaaagatga atgggagcgg
    28861 cagcgcatgt ggctcccgtc gatgttcacg ttcaaggtcg acggcatcag ccgcgatcca
    28921 gctgagacgt gcaagatcga ttcgttttcg atcaagcaag agatcatcga aaacccgatt
    28981 ggcaagttcc tcgaaaacca gaaggaaccc ggtcgcgtcg agtttccgac gattggcgtg
    29041 acgattcttc agcgtgacgc cgggccgtgg atgaagtggt gggatgcgct ggttcgcgag
    29101 ggcaaacacg ttctgagcca gcagcacacc ggccacattt ggtttcttcc gcgcgacgcg
    29161 gtcggctcgg gtcagcttca gcgcaagccg ctgctcacac tcgatctgtt tggcgtgacg
    29221 attttgggac ttggtccggt caagcacgac tcgaagcaga accagcttca gaaggtcaag
    29281 ctcgacctcg cctgcgagcg catcgatctc aagggcggcg aaggcaccgt ctgattgttc
    29341 ctttcggcac tgtacttctc gactccaatt acggcctaga agtcagctag tatgggcaga
    29401 agctctctgc ctggcttttt atggcgcgcc gcaaactgac caacatcgga aactatcgcc
    29461 tcgtgcgcga gattggtcgc ggcggcatgg gcaccgtcta cgaagccgag cacgagcggg
    29521 tcaaaaatcg cgtcgcgatc aaagtcctgc accccgaata ctgcaaagat ccgcagattg
    29581 tcgcccgttt ccgtcaggaa ccgatggcgg cgaacttgac ccggcacgct ggcatcactc
    29641 acgtctttga aagcgatgtc ctgcccgacg ggacgccgta tctggtgatg gagtttctcg
    29701 acggtcagtc gctgcggatg cggctgcgca aagcccgcgg cggtctgccg cagcttcagg
    29761 tgattcgttt tgcccggcag cttgccgacg cacttggagc ggcgcacagc aagaaaatca
    29821 tccaccgcga catcaagccg gaaaacatca tgctggtgcg cgacgacgca gtgccgggtg
    29881 gcgagcgggc caaagtgctc gattttggca ttgcggtggt cgctgaagac gcctcgctgt
    29941 cgatgtcgtc ttcgtcgaac acgcagatca aaaccagtcc gtttgcgtcg cttttgggta
    30001 cggccatcta catggcgccc gagcagtgtc ttgaaagtgg gcgggccgtc gtcgacgaaa
    30061 aaaccgacgt ctatgggctc gggattgtga tctacgaatc gctgtgcggt caggccccgt
    30121 ttgtcgcgcc cgaaatggtc agcgtcatgc acaagcacat ctcggagcca ccgcccgcgc
    30181 tgtcgctttt gcgcgccgat gtccgtcccg agctggagtc gctggtgatg cgcatgctcg
    30241 ccaagtcggg cagtgaacgt ccgtcgatgc gcgaggtatc ggcgcagctt caatcgatcg
    30301 aactggcgga agcggacccg tcggtgggtc tatcggtgcc gccaccgccc agctcgatgg
    30361 gttcgcggct gtggcttggg cttgtgctgc tttttgtgct ggccggggcg ggcactttgt
    30421 ttttctttgg tcgggcgtcg tcggggacag tgacgtggcg aattgatagc agtccatcgg
    30481 gggcagagct tgtgaatgcc gccggtgaag tgattggcaa gacgccgtac cggcacggtc
    30541 ccaagcgcga cgtcggcaag ctggcggtga tgctgcggct ccccggctac tttcccgaga
    30601 cggtgacgct cgattacgag cacgacagca aacagcttgt gcagctacgg aagaagcctc
    30661 cgcagtaaaa ggaattccat gcaaacgact gggttgcgag ggctgggcgt cgtcgctgcg
    30721 atcgcctttt tagggatttc gggctcacat gcccaggtgc cggtgcgccg ctcgccgatc
    30781 gattcggtgt attcgccggg aatgggcgtt gccgacggcg cggaaccttg ggcggtgacg
    30841 ctaaacccgg cgtcgctcgg cgggctgcgc ggccacactt ttggtctgcg tcacagcgag
    30901 ctcacgtccc agatcccgtg gtcgggtcgc ggcaccgggg cgtattttgc gtcgccactg
    30961 ccgtatcttt cggcgttcaa agtcggtggg ggtctggaag tgttgcgacc ccatgacgac
    31021 ctaagcatct tgggcaagct gacggtggcg atggcgtacc agccggtgtg gtgggcgtcg
    31081 gtcgggcttc tgtatgggca tacgtttggc aagccagggg atcgcagcta cgccgggctc
    31141 gatacggttt cttttggtct gcacctgcgc ggcaatcggc ggctcgcgtt tggcgcgatg
    31201 ttccacgatc tgaatgcgcc gaaaccgacg gaccccgcct ggctgccgca ggtttcacgc
    31261 agctatgaac tggaagcgct gcttcagccg ctgggcgatt cgcgctggga cattggactt
    31321 ggcgtgcgca taggtgaagc cacgccagag ctcagcccgc gactgcgact gtgggttcgt
    31381 ccgacggcgg gtcttgggat cggcatcgaa ggcagctcgg tcatcattcc cggcagtggc
    31441 gatccggtgg cctatcgcat cgggttcggg cttggcattg atttttctcg catcggcgtg
    31501 tcggtgtttc agctggccgg gtacggttct gatgcgcctt tggcctatca cggtggttcc
    31561 gtcgcggtgc gcatgtccca agaacgctat ccggcgctgt ggtcgggtcc gcgacaggtg
    31621 ctgcggctgc acttggggga agtcagcgat cgggcgctgc tcaagctgct tctggcactg
    31681 cgagatcaaa aacaaagcca gcagatcgaa ggcgtgctgg tgatcgtaag cggcgtttcc
    31741 ggaagctggg cgacggcgta tgagctgcgt caggcgctcg tcgagctgcg caaagcgggc
    31801 aagcgggtca ttgcctatgg cgccgatctg gggacgcgcg aatactacat tgcctcggcg
    31861 gcagacgaga ttttgctcga tccggtcggg ctcgtgcggc tgtccggcat tgcgcagggc
    31921 accactcatt atcgcgaagc gctgcttcgt ctggggattc gcgccgatct ggtgcggatt
    31981 ggtcgcttca aagcgtcgcc ggagagtttt acgcgcgacg gaccgacgga actgacccgg
    32041 cttcagcggc agtcggtcgt cgatgatctg tcgcagcggg tgctgggcga aattgccgag
    32101 tcccgccacc tgccgctcgg cgatgtcgaa cgcctggtcg aacgcggtca gcagctgcca
    32161 cagctggcaa agtcggcccg gcttgtcgac gcgatcgcca gccaagaaga actgcaaaag
    32221 cggctcgtgc agctttacgg tccgtcggtg cgtgtcgcga acttgccgcg tgagccgcga
    32281 tcgccaagtg ctcgtccaca cggcgtcgcg gtgattgaag tgagcggcga cttgaccgac
    32341 ggccagtcgt ttgcgctgcc ctatgtcgac tttcgtaccg tcggtgcaca gtcgctgtcg
    32401 gcggcgattg agcgtgcagc gcaagatcct cgaatcgaag cagtggtgct gcgcgtcgac
    32461 agccctggtg gctcggcgct cgctgccgat atgttggcgc gtcagatcaa gcagcttgcg
    32521 cagcaaaagc cggtgatctg ttcgtttggc gatctggcgg cgtcgggtgg gtactatctg
    32581 tcggcgccgt gtcgggaaat ttttgccgat ccgctgacca tcaccggatc gatcggcatc
    32641 tttggcggca aagtcgatgt aagcggtctt ttggccaagt ttggcgtcga gcgagcgcgt
    32701 tacacaagcg ggccacacgc cgacatggaa agcccgtttc gtgcctacag cgacgaagaa
    32761 cgcgagcttt tgcgggaacg gcttcaggct ggatatgacc gattcctcga tgtcgtcgaa
    32821 tcggggcgca agctgtcgcg caaagagctg gagccgttgg ccgaaggtcg ggtgttcagc
    32881 ggcggtcaag cggcggcggg gcgtctggtc gacaagctgg gcgggctggc cgatgcggtg
    32941 gcgcgggccc gtgagcttgc gggactagcg ggtgaaaacg atgcggcggt gatctttttg
    33001 cccgatcgac cgcgatcgct gctgcgggaa ctggtcgacg ttgccggcgg tctgacgcag
    33061 gaatcgaccc agcttctgcc ggggccactg cgcgagctga ttcggcttgt gccgcctatg
    33121 ctgtgggcgc tgtccttcga tgggtcggat gtgcttttgc ggctcgacga ggagcccgtc
    33181 aaaccctaag cctggcccgt cgcgagcttg cgcagaaccg cttcgatgat cgccgcttcg
    33241 cttcgttcgg acggcgcgat cgacgggggt tcgggcgggc gcggaatgcc gagtcgctgc
    33301 gcgagctgat ccagcttttt ggcttcgggc ggtggcagcg gcaccacttc gccaatcgcg
    33361 cgcgcggcgt gaatgatctt tgccgagtgc tcgaccgctt ccattcgctg ccaggcttct
    33421 tcgacggtgc gacccatcgt cagggcacca tgtcggtgca tcacgatcgc gttggccttg
    33481 cgcacatacg gtcgcagcga ttcgggcact tcgcttgtcg tcggggttga ataaggcgcc
    33541 gtcggcaccg agccaagcaa aagacaagtc tcgctgagca cacagtcggt caatgacacc
    33601 ccagcgagcg cgagcgccac cgtgatcggc gggtgggcat gaaccacagc ggctacatcg
    33661 ggtcgctcgg cgtagaccag cgcgtgcatc gcaaattccg acgacggatt gtgcgcgcca
    33721 cggagcctgc ggccctggag atcggtgaca atcagctcgt cgggtaaaat gaagcctttg
    33781 tgaaatcccg acggcgtgac cagcactcgt tcatcatcga gccgcagcga cacattgccg
    33841 tcccccgctg cgatcaggcc tttttgatac agccgctgac agacgagcgc gagccgctca
    33901 cgcagcgctg gctctttggc gggtttcgat ccagacatca ccatgcgcct ctaggcgagc
    33961 aggtgccgct cgatttcctg caccatgccg cgcagctggg gatcggtgtc gagcagcgct
    34021 tcgactttgc gacacgccga catcaccgtc gtgtgatctt tgccgccaaa cttgagcccc
    34081 agttcgggaa agctggcttt gcagagcttg cgggccagat acatcgcgac ttgccgtggc
    34141 tttgtcaccg acgacaaacg gctcgaacct ttgagatcgg cgatcttgac cccgaaatag
    34201 ctcgcgactt cgcgctgcac cgcttcgacg tttaggtgcg ccgccgactg ggtcaaaaag
    34261 ttgcgcagcg tgtcttgggc aaactcgatg gtgatcggac gctgctgaag cgatgcaaac
    34321 gccgcgacgc gaatcagaag cccttcgagc tcgcgcacgt tcgacttgat gtgggtcgcc
    34381 aaaaacagcg ccacttcgtc ggacagcgcg atctgatcca gctc
//
LOCUS       DAORGN010000025        34237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_462_length_34237_cov_6.148733, whole genome shotgun sequence.
ACCESSION   DAORGN010000025 DAORGN010000000
VERSION     DAORGN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 34237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34237
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_34237_cov_6.148733"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1827)
                     /locus_tag="PKL17_04800"
     CDS             complement(178..1827)
                     /locus_tag="PKL17_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNK44077.1"
                     /translation="MAGWLGAASLGLGAISGCGGSDGYFGPTVRLHGSFNGWGRSDAP
                     AELSWDGQNYRGVVTLPGETIEMQVYVPSLLSTYGSPQAAKAAPVPAQLSVAKDQAAG
                     GTIRVALPLPARYEVVFSPQNQTLHVDFSDDAADGQSPEAQIIIGALRKSDQLTRAEQ
                     HAKGIGLGDALRALGTDLPLPVSFGELRGLSFWHLGAVDWPSLSLVGDFNGWRTDADP
                     LTFVLDGTVAYLGKRASGARLEYRFDLHGQRYPDPQNPEVVWDGSYLPPNPANILGGN
                     AGELNSVAYAPGYLEPGARLRRLSGPDAPEGQPRPEVLVYLPPGYAQAATTRYPVLYV
                     LDGKDAVVRGGYARLFERLSQAGKLPRIVGVFVSSPSDSQARLSALTSYRDPAYPEID
                     PRGDAFSRWLTDGLMPQVDKTLRVTASRALLGVDIAGPLVMGLVWKDDKKRFLRAMSQ
                     SGRFGWGDPQLVNSPYLKLMATDISGRIERLAFDYADIDHPQAQVHETTLRSALSATG
                     YLSKVQFFKSTATAMDTWDSLRQRAEVSLPFLLRDLSPASK"
     gene            1951..3840
                     /locus_tag="PKL17_04805"
     CDS             1951..3840
                     /locus_tag="PKL17_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HNK44078.1"
                     /translation="MGRSTLNGHMFARRQAPRSIGKLGAAGRVLALAGLLVGASHVER
                     SQAKPPDPDPLPWPTIAVKDIRPGMVGYGLTVFSGTKPERFPVRVVGVLPKHTSLMDI
                     ILVESDDPRLKHSGIVAGMSGSPIYFDGKLAGALSLGWAFSKDALGGVTPIEYMAADL
                     KRPQRGRDHNHIARRSTDATRQNNPIEALHHEIPVLAQLAPGDTLQPATVPLLVSGLG
                     PQSLDLLRSAFLPYGITVMQGGGSATANAAAPRNFEPGSAIAVELVRGDVAMQSIGTV
                     TAIAGNRVLAFGHPMLNAGETYFPVATAEITSFMPSLISSFKFGHSLATVGSLIQDRS
                     AGIIADTSLPAQTLPVTFSIHPVGQPSRTLRTEMIRHRLLTPVLVGVVASQAVSVTAS
                     DVADAVVRIDSTLKVAGFAPLKQTDYLLASDGFSGKMLANSTGIRQLQEVLGNPIEPV
                     KVESLDISVELIYKPQAADVVSFSLPSDELEPAQTVPLRVLLRPFGKPVEFVTIPVEI
                     PRALAGQTVKIEVQAGPQVKPELASPDSIGDFIENLRKGYSGKSLVVTLNSTDEGVAL
                     RGRLVPSLPASVMATLRPGSNSRRGEVQKRLYRTVHDVDWVVQGKQELTIQIKELRED
                     TRSTP"
     gene            3837..6044
                     /locus_tag="PKL17_04810"
     CDS             3837..6044
                     /locus_tag="PKL17_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44079.1"
                     /translation="MKTRSPLIFAATFAWLAMAGVPEPASAGGTRSIRVTTYQEFAEG
                     QDQGVLITSQGDVQSGWSTTKTDLPQVGDDAIRTMVAAPDRTIYIGTGGESPNVHIYQ
                     AGKLRKLAKLPTTTWVSALAVRATPTGNHVLAATVADGRIFDIAEDGKVELWATVEAD
                     HIWAIQREGNQTIVATAPGRLLLIQDTDCGPTAKLGANQKTKSKILFESPARHFLSLA
                     KGEPGVYYVGTSDEAILYRITLDKEGRATARATHDFAGNEVRAVAAKDGKIFVAVNDM
                     QRGDTQLRGTKLTVPAAGTAPGVKPAPPSGTTAPPSTSPVEKKGRGGLFRIDEAGRVE
                     QLHAISDGFFNDLAVDEDGSVLAAASTPGGRGRVYRALPDRTVMTALEFKESDVLALA
                     WSQTERIVGTGSAAGLFQVQTKPATGASYQSKVLDATAIARFGALRFLAEGVRVETRS
                     GNVQKPDAAWSAWQPLAKVARVALTGEQTGEIGSPPGRYLQVRFLFAEKSVLKDFSVY
                     YQPLNQRPRLTDVTIGEDPLSRLAKGVKPSYLRPKSPIVKIRWKVENPDEDDLNYRVF
                     IRRIQSTPAAPGGPAGDVGFLRISGVDPLTRTELDLNTETVGDGLYEVKVTVSDERSN
                     QTDLALTHELSSPPFLIDNRRPEWLDVRFDATSQLVSARITDSASPITDLSLSIDGGD
                     FQPLTSRDGILDSPSEEIEYKLARLSPGAHTLLLRGSDAADNVATTQLVIQSK"
     gene            6105..7037
                     /locus_tag="PKL17_04815"
     CDS             6105..7037
                     /locus_tag="PKL17_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNK44080.1"
                     /translation="MPSVSYALRFISGKYQGGEFPLHKGREIVIGRSSELEMVLIEDM
                     VSRRHAAITINGDELTIADVGSTNGTFVNGEKIKRARLKEGDRILIGTSIMKVVTVDS
                     NMTEAEAKSQLEKRGQQQMQKTSAATRAMTGNIEEIPLPDLLQLFSTSKRTGVLTVNG
                     PNSSTGQIYLRKGQLYYASIDDSYEMKPEKAAYRILTWDKGTFELDSTEEKTFLEEIK
                     DSTEGLLMEAMRIVDEMQRLDPDLPARSAQLVIPKPLVPSLGELSKDQLEVFQVILNG
                     ATTQGVLDKSRQADCDAAQVLVDLIKKEYVVART"
     gene            7122..8282
                     /locus_tag="PKL17_04820"
     CDS             7122..8282
                     /locus_tag="PKL17_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK44081.1"
                     /translation="MKTIVAAFHPTPPSELHQGLLHRHDIRLLHVSTLPDLVDRLGKG
                     ADLCLLGPQLADCGAATASQTLRSHRRGHPIPIIFIHTGTAHAVALPPGLFDEVIEWP
                     AQQSHLPTLLARYLGVAARASERLPLKVHVYQGTSGTATNHPDAFLGSSIDISMEGLL
                     LRTSRDVGVGDKLNLRFTLPSRVQAVVCLGRVIRIDTRTHAPDLAVALHFESMPEETR
                     SALAEFFKSASGRAFRWKIIRDGQRQIVKLSGVLSAEVDLSPLKQLKGEIDFNLREFR
                     RISSDSIQTWLDLVRSLRGASKIRLVECPIQFVQQASAISNLLDSTEVVSFFAPYVCP
                     RCGLDEEQLIDVRRDLYSLQGTLERKPPSFGCVRCGTAMMFDDIPERYFMFL"
     gene            8334..10793
                     /locus_tag="PKL17_04825"
     CDS             8334..10793
                     /locus_tag="PKL17_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44082.1"
                     /translation="MPTLATPVTVRFADQNGTAVSLEGVLLSVGETATTELQGNQTRK
                     GLHLLLSGDRDYYVMISQPLAPDTRLALSGQVGKSVSALFTGRPEVRRRLARLIESTQ
                     DPEAQPGTPAEGGPSPEASTQGAPINLQEGIFGAAPLFLLPEGRLSLSATDPVPTSIE
                     LTPVFVAAARWISSRRRSTFECLFPPSAFHPDLPVRPDRLSVAQAGVLLVQLRDGLSA
                     AAVGGEGANENPVEAVQLRSAALTILSHLVATVAKDPSFRTVADEAAEIIFSLIDEEK
                     DDPTARPALRSHAIQLLQMRAPALSAAHRQRAQDLLRSLIRQAPPYTELPTVWRFAMC
                     SAWDFHEGEVELLQKRFGFTKAEPEKNPPKLPFPVERYHVVQAPFHGPKGEPIYVYAR
                     TASPKDENTEMGSEAFVGVLINRHAQLGSFDMQASASDVHQVGYKLMMNSQCAGLTTR
                     FAIAKMFPEADIYSSWDSTYFRTDPKSGKVTASEGLDCFAALLEGMSRGESHDDLSAR
                     IQQAQWHHEQGQRDGFVQFVGPAHPLVISRYSDVNQDGKADFYDGFLDFTLKNIQTDL
                     QASATPRDPGVAASQIGGDAATGLNWAAGSLNRVTQYSDLWRGLPGDSELFYIFSSGG
                     FYSHLEPPQDVAVGQGAQEVELGRLPAVCRYIQKSYGSADEPGLSVEVMFHSHLSHAA
                     PELKRLLCAAEAMRRGLDLGMLPPEGPLATQLAQRGALLLLLAGLLEFPADQNFIDGL
                     WSMALKLLNLPSISRSLVRGCITDADHNASNYYGSRRGLLQLLGDGTTDGALTKSDPL
                     AYERLRSDDPSIGRAIPLKLS"
     gene            complement(10806..12491)
                     /locus_tag="PKL17_04830"
     CDS             complement(10806..12491)
                     /locus_tag="PKL17_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK44083.1"
                     /translation="MTRLFLLLFGLWGLGLCACRNTEVVASPPLLDDGQLAKVQAAQE
                     GELKRTLGGRLRYHRELKTVAHADSLAVTLPSAGSLASSLIRLLLRYPSLFGSPSSDQ
                     FESVETTTESDGSRAITLRQKSQDVTIWGSFLRGHLDRAGKLKRIVAHTVPLLSWRAE
                     AAIPQISTEAARQESLRILTAERPLVAFSAQTPKLYYFPVEKQLFLVYRVEVSGQDGD
                     LPIRLALFLSAHTLSLLWREDLVIATDVPMSTIGRGTGVLGESFELSITQRGDSYSLE
                     DIRRGSARATVAKAAERLPGKTVQSDRVDGWDVPSAVSAYAHLALLWDYFSTTHKFFG
                     WDGSGHGIVVATHSEVRFPPLPVALFDGQRIVLGAGSPPSLLPAGGAFDVVAHEYAHA
                     VIRATADLAAEGESGAIDEGLANIFACLSEHAVGIARPNWTVGDSIYRPLTGTAALAD
                     LEDPRRTEQARFATDKVPVELRLASSPVLTDRSLRRFRAGYIGHVGFLISAKLGPAKT
                     ARLYFRAVTTYLHRYADATDLADATIAASTDLFADSESVQAAWDSAGVSDYLR"
     gene            12659..14695
                     /locus_tag="PKL17_04835"
     CDS             12659..14695
                     /locus_tag="PKL17_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44084.1"
                     /translation="MLRTSSPFQRVRSTFRRHLRPSTLCASFSSLLLLTSCGGEDPSA
                     GPGALALHVLPGLSSGTMNDYFSLPFPNDLRLKTQPAPTAPVGESELRSGYDLTQFPQ
                     PAGQPGKYVAFLNGKIPGAGPGAAIYFRFDAPLDTTTLPAAERSTDDDSTAFVVDVTE
                     GSPTYLKRTPILATFHREGNRYIGDNWVSLRPVPGLPLRERTTYIAVLTKGVHADAGG
                     EIRPDIPLLAMMSVTQPMNEPYRSAWPRYAPLRNWIAATGLRDKVGAATVFTTQDATS
                     MMGKLRQAVYDQTAVPTPKAVTYLRSHDGMADLYTGTFASPNFQSGTPPYWSEGGGLD
                     LDARGIPKVVRTENLRFALSIPNAEMPPDGWPVILYAHGTGGDYQTFIREQLDLRASL
                     IEQAGRPPVRMAMIGIDQVLHGPRDPSSSNPEITFFNLQNLEAARENVKQGAVDDFQL
                     LRLVEGMDIPKAPTTGRPIRFDKKRIYFMGHSQGGLTGPLFLAAEPKVPAGILSGAGS
                     VLILSLLNKTEPNNIAELVESLLQEPPLVDHPLLNLLQAYFESSDPNNYGKLYFREPP
                     KGMAPKSIFQSLGLWDHFTPVPNIAAFALAMGVTPVEPRLYDLPYLSLTDLRWKNGKV
                     AGNVADGKATAVLKQYVAPPRDDGHFVIFDLWAARHDWSRFLASLAQGGLSVLE"
     gene            14700..15734
                     /locus_tag="PKL17_04840"
     CDS             14700..15734
                     /locus_tag="PKL17_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNK44085.1"
                     /translation="MSRNKQLVLLHRPTGRVSIDHFEVREIPLRPPSDGELLIKTLYL
                     SIDPTNRVWMSDRPQYMPPVEIGEVMRGFGLGEVIDSRHPQFARGDLVTGLLGWQTHC
                     YSDAQGLRPLQKIPTDTGIPPRTFLGALGLTGGLTAYFGMISLGDPKAGETVVVSAAA
                     GSVGSLAGQLAKLRGARVIGIAGGHEKCSYVVGELGFDACIDYQNQDVGTELERLCPQ
                     GISVYFDNVGGSILEAVLARLALRARVVLCGMISQYNDEQRWTLPRNFDAILYQRARV
                     EGFIVSDFRPQFPKAYAELIPLWRSGQLTYREHLVHGLENAPAAVNMLFDGKNHGKLI
                     LQVATDGTSS"
     gene            15750..16532
                     /locus_tag="PKL17_04845"
     CDS             15750..16532
                     /locus_tag="PKL17_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK44086.1"
                     /translation="MSAKHVVVTGASSGIGAALVTEFVRCGARVTMVARRQAEMEALA
                     SQRPGQTQFFVVDLSRTETALDWIAKAEATFGPIDVLIHNAGVQLVEPAEEHDPEALL
                     AMLRLNLLTPLAITRQVLPSMVARGDGTIVDIASVAGLAPTPGMWGYNASKAGLGAAS
                     ESLRGELRQSGVHVVTVYPGPVDTPMARAGYAAYPENPALRVLPEGRPSELARLIREA
                     VDLRRPRVVYPSFYALLRYIPTVARWFLDRMTPRPFSRKDRT"
     gene            16532..16729
                     /locus_tag="PKL17_04850"
     CDS             16532..16729
                     /locus_tag="PKL17_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44087.1"
                     /translation="MRVSRDARFDRERAEFALRHCCEDCGLFVAQRNLCAHEWPTEEH
                     RRADYEKPTADLLFCKEFELA"
     gene            complement(16743..18005)
                     /locus_tag="PKL17_04855"
     CDS             complement(16743..18005)
                     /locus_tag="PKL17_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK44088.1"
                     /translation="MNRLSVAVLAVPLLSGCLVENLCERQPDLAECQVTQVEGPARLV
                     SRRLPLLGGELVVQVMGSPESVDVLLSPAGLDEKRVPLRLMGDGLWHAMAKPQELWPT
                     VLPGPVPIKVVTPNQQTAGTLRLYVPPSFGPDVSRSAKIVWLSVVSRHVIALEQGVTD
                     RTFGDWTYQPPVLTKAASVPLSMAFTVPSTALFGSTETAFLRLIPSGAGFALQQSPLG
                     ELRYSDIEASLDYASVTALAAGKRGAGNLLAVAGEGGSGPFLRAYRLPDGGSAATSRL
                     TVSASTGPLLLISAGTLDDNDSVDLVSVGKDGSAAVWLATATSLARDESLSQSLSTQL
                     GSDPVRALSIGDIDRDGLADVIISRKSQLSWLANQADGSFAAPSVLLTLPDNATTIDV
                     GLLDGNDSPDVVLGGPGLVLISYLNQAQ"
     gene            complement(18016..18861)
                     /locus_tag="PKL17_04860"
     CDS             complement(18016..18861)
                     /locus_tag="PKL17_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44089.1"
                     /translation="MSRSGVASVLRRIYLFGLFLCSWPSLVSAADEPVVLIGVFRDNR
                     QDGVLQSRLRDHLARNGELLLPTPKLGPGEQQCTSQECLDRLAAKAGAKFVLSIAAQR
                     TAATGDGLYLTGLLYDASRHWPRQATLDCMGCKGELLVTRITELADKLFFEIRTPELT
                     LAMNQPALPKPALVESPVSLAGSHGGSEGSYFDRLSARRKVAAGVLGAVSLAVFATAG
                     AFHGLDGHRTDIRCAPTSVPQACAWNTKWFYGAGYGLGSALLIGAGLTLFWPAETSST
                     VAAHP"
     gene            complement(18874..19152)
                     /locus_tag="PKL17_04865"
     CDS             complement(18874..19152)
                     /locus_tag="PKL17_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNK44090.1"
                     /translation="MSATSQEPEIDFVTFVMSLAASALLHMGEHEEGAPAELRDTPID
                     LAMAKQTIDIIGMLREKTRGNLTADEDRLVETALYDLRLKYLHRSKQS"
     gene            complement(19168..19482)
                     /locus_tag="PKL17_04870"
     CDS             complement(19168..19482)
                     /locus_tag="PKL17_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44091.1"
                     /translation="MADLSNDPILWDVRILERRLRRGEVSDKQLQAYLQTLPDVADKA
                     TASIPLEEPIERPPPRRPQVRITAAPAVRRDMDLDSDDLDSDLIDDDDDDDDDDLDDD
                     DE"
     gene            19612..20946
                     /locus_tag="PKL17_04875"
     CDS             19612..20946
                     /locus_tag="PKL17_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNK44092.1"
                     /translation="MQSSARPLDCRHYMPLGAAGEEAASNGLSKLALQLVGSEILKIA
                     AAVRALQAQGHAVCNLTVGDFLPKQFPIPPKLQSAVIRAYELRETNYPPSDGMPAMRE
                     AVTELYRRTLGLDYPVESVAIFGGARPAIYGTYRAIIDPGERVIYSTPSWNNNHYVRL
                     ADAIPVECETSPEEGFMPTAKSLRPLLAGARLLCLNSPLNPTGTVIDKDELRRICELV
                     LDENQRRKRSGERSLYVMYDQVYYALTFGQAKHHTPPELCPDMAAYTVFVDAISKSLC
                     ATGLRVGWAVAPPYVMGRMRDILGHVGAWAPRPEQVATAELLRDPDGLATFQTQMMAE
                     VEQRLSLLYDGISRLAAEGLPVRAIPPQGAIYLSVQFNLHGRKLPDGTVVETNEQVRN
                     YLLHAAGMAVVPFQAFGMRAETGWMRLSVGAVSIDDVTKLLPRLREALFAVA"
     gene            20957..22702
                     /locus_tag="PKL17_04880"
     CDS             20957..22702
                     /locus_tag="PKL17_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44093.1"
                     /translation="MNRSKPSLRPTFALLASVGFLLGAGCSVPTDKLPLGPIPLVLEN
                     GLLYARGQLLPTRDERCGQTPEEGAPPGSGLDAILIDTATPLTALVPQEADTPRQFQH
                     GQFSLYAALAGGKSGATQLLRCDVPVVRSDLSARDFGLRWDSSPKLLLGGVIGADQLL
                     NYSLELRLATDRPTIQISRGDLAPWCRVDRAVIPFKPLGGELAVQLSDTVIQYPATRI
                     TVGACVEPLSDPLSGPRPVDCISDADVDAALQRVLLAIRTLESQQPRDEEQLTRLASD
                     EALLRGLKLGQCTDDKSTQLGDLADNQRLRRAPYEVSGTNMRFLVSTALPELVLTQTA
                     CQRLGGKTCSCDEKNRVPLLLPGLNAKRQSDGSVVDSPDYGCRIQLGYSGHAALALMA
                     KQRQLSPCDELARSRRQRWAMSPAPAGATMQNICQRSLCLRNLGRDPAQLNRRCGYTG
                     LDSDLACNDKLSPVAAAVELGGPIDDTTTDTLSAVVVPDSAALVQSANADVRNLSAQL
                     DGVIGVSLLRRLYTTIDYPQGRIELLCRCNDPLATQKTVCQAYRGITYYTADACVESD
                     TLTVPSDLARLRCDG"
     gene            22746..24560
                     /locus_tag="PKL17_04885"
     CDS             22746..24560
                     /locus_tag="PKL17_04885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNK44094.1"
                     /translation="MMKLACTPCSAPAGRLAFFATLLLSATASAQPADLVIQKAHVLT
                     MDGARPTATTVAVRDRRIVYVGDEAGVSQFIGPRTRVVSSSGSPDMTLLPGLQDAHAH
                     LVGLGMSLAALDLRGLSSVEAISQAVTKAAQEHSDRGQFVIGRGWDQNRFSPPNFPDR
                     AQLKRLDEASAGHPVWLRRIDGHAGWANSAALALAGIGRKTVDVAGGRILRDDSGEPT
                     GVLIDNAMDLVERVLPQPSGKEIEAAIVRGSGYVVARGLTAVHDMGIGPDAVAAYRRL
                     AEQGRLPLRVYAYAEDPVPSKLQQLPHSLAYRAELDRLAQRLGPPDQSGLFTLRGIKL
                     FLDGALGSRGAALEQPYSDDPQNRGLLLTSPDHIELMARWALVHGYQLATHAIGDRAV
                     NLVLDAYRRAGVSASRNARFRIEHTQVVSDEVISQRQLQSLGVIASVQPQHAPSDAPW
                     ATQRLGPDRIKLAYAYRALLDSGAHLAGGSDFPVEEADPRLALDAALWKTQAGKPGSK
                     PPADALSVGESVRLLTSDAAYAAFAEDRLGRISVGMLADLTLIEGKLSLDDKEPVPSD
                     LPRRKVLLTVVDGVVVYDGLGQNQAHRPRRRGRLVRHR"
     gene            24597..25499
                     /locus_tag="PKL17_04890"
     CDS             24597..25499
                     /locus_tag="PKL17_04890"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44095.1"
                     /translation="MPASLMIATEEGLTVELHLSSQPVSVGRAKDNTLRIDDRRMSRH
                     HLVFRRREDASYEVADLGSYNGTLLNGRRIRQESLKAQDIIRCAGLQIQLIEKESAGD
                     AVEPSLMTVTNQALVEARSQIKQMIDEQAMLRREVGLAQESEARAKRLRDEAQDEVER
                     LHDVIAKLGQDKESLAAKVEELGKELRERLATKTDPRTDELTAKLSEAQKQVEKHKAR
                     IAELEEKDAARVASEGPLRKEIDRLADILKKRDQRELELQAAVKPALIRIAELTRELE
                     NTRIELAHSQADLEDIKKALLGKR"
     gene            25564..27177
                     /locus_tag="PKL17_04895"
     CDS             25564..27177
                     /locus_tag="PKL17_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNK44096.1"
                     /translation="MRAPFWLGSVVLFFVAGCGEDSPLEKPEALSFPASFRFGTATAG
                     FQVEMGCPKTAKADCEFPSDWTDYVTRPELVAQKNLYLSGDPPSRGPGFFELYPQDIA
                     RAKQELHSNALRLSIEWGRVFPNPTDGINGYEALRKAADPKALAFYHGVFSALRQNGI
                     KPLATVIHYVLPAWIHDAYGCHTNFAGCKNRGWLDHDRILSEAAKYAGFLGREFGAEV
                     DDWATENEPFTAVIIAGYLFQTEQRTHPPAVVLESDAARAVLNAEIEGHARMYDALKA
                     ADTADADGDGQAARVGLVYNMEAVAPADPQNEIDVSGAKHFDYLLNRVFLNATIRGDL
                     DANLDGKTVHRTDLAGRMDFLGVNYYARNVVQGSPKSFLPQLSPLLNFNILTVEYDWN
                     YARGLYEVLDSVKGFGIPLVVTETGVEDASDSGSAAAWVVRSLTWVSRAIADGIPVEG
                     YYYWTLMDNYEWNHGMNVKMGLYAVDPNDPQKTRRARRAVPIYGQIAQAGQIPKELTD
                     QYKEPRDPPHSDGGKTPTAIRIGAAAKAR"
     gene            complement(27143..27592)
                     /locus_tag="PKL17_04900"
     CDS             complement(27143..27592)
                     /locus_tag="PKL17_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44097.1"
                     /translation="MSLSPPSGSGDAKSLYSDEYACVLLTNEARLIRLKRTAQAYPDL
                     SALLGSYEKVIAAIDRTGRRGRALLVDTRAPSGRNDAWFEQGMASVRPRIDRGFARVG
                     VLTQTRIGTLQIRRWVNEDGIERLAGCDEELLIAELLSGLLPRPRFG"
     gene            complement(27655..29826)
                     /locus_tag="PKL17_04905"
     CDS             complement(27655..29826)
                     /locus_tag="PKL17_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNK44098.1"
                     /translation="MGIRLADLNPPQQKAVITTEGPLLVLAGAGSGKTRVITYRIAHL
                     LDKGVRPESILAVSFTNKAADEMRERVDRLCVGGVARGVMLSTFHALGLLILKQEQAA
                     LGMPTGFSVYDTSDQLGVIREILRHAHLDGKKLDVKLIQSRISRAKNAGLTATDFITQ
                     VRRARFVSEYDGFTAEVYPRYVEKMRALHALDFDDLLVETLRLLSEDERVQKRWQSRF
                     RYLMIDEYQDTNHCQLSLLRILCQQHRNLAVVGDDDQSIYAWRGADSRNILQFEKQFP
                     GAQVIKLEENYRSTGGILKAANAVIAKNSARHAKTLWTQRDDGEPIQLVIAPDEDAEA
                     EFVASEIDSLVISGKFDLRDIAILFRTSRQLEPIEQALRQARIDYHVIGGQAFFDRKE
                     VKDAMAYLKMLTYPHDELSLRRVINYPPRGIGPQAMEALSQIHQKARRRKPDTSLWEA
                     LRDLCGTQTGSLDLFADDSQVPQHDALPPRVMSSLFRFVRTLQKYQKMYAQVGAEKLK
                     DVAEELLRELNVHDDLVRAGPTMLQAERRLRNLSEFLASMQRYAEKAGPDFDMLSYLN
                     RLSISSQDEDVDDSLRDEVTLSTLHGAKGLEWKVVFFVGLEEEILPHKRALAPREADH
                     TIAAQRHSDGNSDGEDPSSDISEERRLCYVGITRARQKLYISRSELRGGWPKNPSRFL
                     EDIPDSLTVLRDLEGPPPKQDPQDEEAFIKSLIAQARAVSE"
     gene            30018..32093
                     /locus_tag="PKL17_04910"
     CDS             30018..32093
                     /locus_tag="PKL17_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNK44099.1"
                     /translation="MATPAEPKLTYPPTKTVDQVDEYHGEKVKDPYRWLESLDSEETR
                     QWVEAQNKVTFSYLEKIPGRDKLKQRLTELWNFEKYSAPFREGNRYFYSYNSGLQNQS
                     VLYTLTSLDAQPQLLLDPNTLAKDGTVALAGMSLTHDGNLMAYGLSAAGSDWQEWRVR
                     DVQTGKDLPDVIKWIKFSGAAWTRDNKGFFYGRFDEPKNTGKSLEDVNFFQKLYYHRL
                     GTPQAQDTLVYEDKQHKEWQFAPQVSDDGKYLIVTVAKGTDDKYRILYQELDKPGAKF
                     VELINNFDAEYTFIDNDGPVFFFKTDLKAPKAKIIAIDVRKPTERKELIPESQDTLQH
                     VSVVNGSFVATYLHDATTSVKVFDLNGKFVRDVQLPGLGTSMGFAGRRAYKETYYAFT
                     SYNMPATIYRLDLTSYESKVWKQPQLKFNPQDFETKQVFFTSKDGTKVPMFLTAKKGI
                     KQDGLNPTYLYGYGGFNIPILPSFSVADLVWMEHGGLIAYVNLRGGGEYGEEWHRAGT
                     KLRKQNVFDDFIASAEWLIKNKYTSSSKLSIAGGSNGGLLVGASITQRPDLFAAAIPS
                     VGVMDMLRFHKFTIGWEWIDDYGSSDNPEEYKALRAYSPYHNIKPGTAYPATMVTTAD
                     HDDRVVPGHSFKFAAALQAAQKGNAPVIIRIDVRAGHGAGKPTSKRIDEAADRLAFLG
                     KNLGIDFSK"
     gene            32106..32648
                     /locus_tag="PKL17_04915"
     CDS             32106..32648
                     /locus_tag="PKL17_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNK44100.1"
                     /translation="MAGSVNKVIVIGHLGANPELKYLPSGQPVCEMRVATSETYRDRN
                     DQPAERTEWHRIVVWGKTAENCSKFLQKGRQVYVEGRLQTRSWDDKEGKKQYMTEIVA
                     NQVVFLGGGGREEGARESGGGGYSRESGGYAREAAYGGGGGGGGGGGGGGRYGRSAPA
                     EAAPMAAEPGDFAAEDDIPF"
     gene            32658..33392
                     /locus_tag="PKL17_04920"
     CDS             32658..33392
                     /locus_tag="PKL17_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNK44101.1"
                     /translation="MFCWYELRTTDVSAAQRFYAAVMQWETRDPPGPGLFWQADRAVA
                     DVSPLSLQARQNGAPAHWLGHLSTGDLDRTMGNLIAAGALLRGPVRTGQDGSLAVLRD
                     PHGAMVGLSTDRTKQSQAVSWHELHVLDAEKALALYCEQFGWQPQEVLDLPMGIGPYR
                     MFASQAGKTPCGAVASMAHRPQVHTHWLFYFAVDDLDAACARVVAQGGEVFFGPVEAF
                     GSRRIAVCHDPQGAEFALQASAPIPC"
     gene            33425..33814
                     /locus_tag="PKL17_04925"
     CDS             33425..33814
                     /locus_tag="PKL17_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44102.1"
                     /translation="MSVRRSVAPDIAGCTFVLFAVTLSFAVLRAPFIHKQAGAMAQDL
                     LSYGGLMFGCMLTAAGLFRRKLWAFFPAVLLSVVAIYAAAINWHMPETGQLPAGVLGL
                     TTCVLLLLRYREFGGETQPESSRQSAE"
     gene            33991..>34237
                     /locus_tag="PKL17_04930"
     CDS             33991..>34237
                     /locus_tag="PKL17_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44103.1"
                     /translation="MSEAADKQKRIRRGALAPNPELWAALEQGEKLTAILTEFYTLAF
                     VDPRLSPFFRDVTKQRLIEKQYNFMYEILTGEPVYFGE"
BASE COUNT         7140 a        10350 c        10133 g         6614 t
ORIGIN      
        1 gcgcccgatc tgcggctatg aaagcataca accacctttc atttcaaggg gttatcgcga
       61 tcggggtgca ggccgggctt gggaggttaa gttctcgacg cggagtggac acagaaaacc
      121 agcgcacagc gaaggttttg gccagccagc gcgaaatttt tttcgcgaaa atttgactta
      181 cttgctggcc ggggaaaggt cgcgcagcaa aaatggcagg ctgacttccg cccgctggcg
      241 cagcgaatcc caggtgtcca ttgccgtcgc tgtcgacttg aaaaactgaa ctttgctcag
      301 gtaaccggtt gctgacagcg ccgaccgcag cgtagtttcg tggacttggg cctgcgggtg
      361 atcgatgtcc gcgtagtcaa atgccagccg ctcgatccgc cccgaaatat ccgtcgccat
      421 cagcttcaaa tacggcgagt tcacaagctg cggatcgccc cagccgaagc ggcccgactg
      481 gctcatcgcg cgcagaaacc gctttttgtc atctttccag accaggccca tcaccagcgg
      541 ccccgcgata tcaaccccga gcagcgcccg cgacgcagtc acccgcagcg tcttgtcgac
      601 ctgtggcata agaccgtcgg tgagccagcg cgaaaaagca tcgccgcggg gatcgatttc
      661 cggataagca ggatcgcgat agctggtcag cgctgacagt cgggcttgcg agtccgacgg
      721 tgacgaaaca aatacaccga cgatgcgtgg tagcttgccg gcctgcgaaa gccgttcgaa
      781 cagccgtgca taaccgcccc ggaccactgc atctttccca tcgagcacat agagcaccgg
      841 atagcgcgtc gtcgccgctt gcgcataacc gggtggcaga tagacaagca cttcgggccg
      901 aggctgacct tcgggagcgt cgggcccaga cagtcgacgc agccgcgcgc ccggttccaa
      961 atagcccggc gcatacgcca ccgagttcaa ctctcccgcg ttgcccccga ggatgttcgc
     1021 cggattgggc ggcagatacg agccgtccca caccacctcg ggattctgcg gatcgggata
     1081 gcgctggcca tgcaaatcaa agcgatattc gagccgcgcg ccgctcgccc gcttgcccag
     1141 gtacgccacc gtcccgtcga gaacaaaggt cagagggtcg gcgtcggtgc gccagccatt
     1201 gaaatcgcca accagcgaca gcgacggcca gtccaccgcc cccagatgcc aaaacgacag
     1261 cccccgaagc tcaccgaagc ttaccggcag aggcagatcg gtcccaagcg cccgcagcgc
     1321 gtcgccaagc ccgattcctt tggcgtgctg ttcggcgcgg gtcagctggt cgcttttgcg
     1381 caaggcgcca atgatgatct gcgcttccgg actttggccg tcagccgcgt catcggaaaa
     1441 atcaacgtgc agcgtctgat tttgcggcga aaaaaccact tcatagcgcg ccggcagcgg
     1501 cagcgcgacc cggatcgtcc cccccgccgc ttggtctttg gcgacggaaa gttgcgcagg
     1561 caccggcgcg gctttggcag cttgcggact tccatacgtg ctaagcagcg acggaacata
     1621 gacttgcatc tcgatcgtct cgccgggcaa cgtgaccacc ccgcggtagt tctgtccgtc
     1681 ccacgaaagc tcggcggggg catcgctgcg accccacccg ttaaagctac cgtgcagtcg
     1741 caccgtcggg ccgaaataac cgtcactgcc cccgcagcca cttatcgccc caagccccag
     1801 cgacgcagcc ccgagccaac cagccatcca gaacctgcgc caaggcgcca caccgttcga
     1861 ccgtgagcga gtcatgaggc cgtatattcc gcgtcgctac ggagtgtcaa gcgccggacc
     1921 ttgggttggg gcctgcattg acggaaagcc atggggcgtt ctactctcaa tggccatatg
     1981 tttgcgcgca gacaagcgcc tagatcgatt ggcaaattgg gcgccgcggg ccgggtgctg
     2041 gctttggccg gactgctcgt gggagccagt cacgtcgagc gatcgcaggc gaagcctcca
     2101 gatcccgatc cgctgccctg gccgacgatt gcggtcaaag acatccggcc agggatggtc
     2161 ggttatggac tgacggtgtt ttctggcaca aaacccgaac gcttcccggt gcgggtcgtc
     2221 ggtgtgctgc caaaacacac gagcctgatg gacatcattc tcgtcgaaag tgacgatccg
     2281 cgactgaaac actccggaat cgtcgcggga atgtcgggtt cgccgatcta tttcgacggc
     2341 aagctcgcgg gggcactgtc gctgggctgg gcgttttcca aagacgcgct cggtggcgtg
     2401 acgcccatcg aatacatggc tgccgatcta aaacgtccgc agcgaggccg cgaccacaat
     2461 cacatcgcgc gccgctcgac ggatgcgaca cggcaaaaca atccgatcga agcgctgcac
     2521 cacgaaatcc cggttttggc ccagctggcc ccaggtgata cactgcaacc agcgacggtg
     2581 ccactgctgg tttccggact tggaccccag agcctggatc ttctgcgcag cgcgtttttg
     2641 ccgtatggaa ttacggtcat gcaaggtggc ggctcagcga cggcaaacgc agcggcacca
     2701 cggaatttcg aaccaggcag cgccattgcc gtcgagctgg tccgcggcga tgtcgccatg
     2761 cagtcgatcg gcaccgtcac cgcgatcgcc ggaaaccggg tgctcgcgtt tggacatccg
     2821 atgctcaatg ccggtgagac ttattttccc gtcgctactg ccgaaatcac gagctttatg
     2881 ccatcgctga tttcgagttt taagtttggc cactcgctgg cgacggtcgg ctcgctgatt
     2941 caagatcgct cagcggggat cattgccgac acgtcgctgc cagcccagac gctgccggtg
     3001 actttttcga tccatcccgt tggccagccc agtcggacgc tgcgcaccga gatgattcgg
     3061 catcggctct tgacgcctgt tctcgtcgga gtggtcgcaa gccaagcagt cagtgtcacc
     3121 gcttcggatg tggccgatgc ggtggtgcgg attgattcga cactgaaagt ggccggtttt
     3181 gcgccgctca aacagaccga ctatctgctc gcgtccgacg gcttttcagg aaaaatgctg
     3241 gcgaactcga cggggataag acagctccaa gaagtgctcg gcaatccgat cgagccagtc
     3301 aaagtcgaat cactcgatat cagcgtcgag ctgatctaca aaccgcaggc tgccgacgtg
     3361 gtgtcttttt cgctgccgtc ggatgagctg gagccggcac aaacggtgcc gctgcgcgtg
     3421 cttctgcgtc cgttcgggaa gcccgtcgag tttgtgacga ttcccgtcga gattccccgc
     3481 gcgctggccg gtcaaaccgt caagatcgaa gtccaggccg gtccgcaagt gaaacccgag
     3541 ctggcctcgc ctgacagcat cggggatttc atcgaaaacc tgcgcaaagg ctactcgggc
     3601 aagtcgctcg tcgtcacgct caattcgaca gacgaaggcg tggcgctgcg tggacggctg
     3661 gtgccaagcc tgccggcgtc ggtgatggcg acgctgcggc ctggatcaaa cagtcgacgt
     3721 ggtgaagtcc aaaagcgcct gtatcgcacg gtccacgacg tcgactgggt ggtgcaaggc
     3781 aaacaagagc tgacgataca gatcaaagaa cttcgggaag atacgcggag cacgccgtga
     3841 aaacacgatc cccactgatt tttgcggcca ctttcgcctg gcttgcgatg gccggtgttc
     3901 cagagccagc ctcggcgggc ggcacgcgca gcatccgcgt gacgacctat caggaatttg
     3961 ccgaaggcca agatcagggc gtgctcatca cctcgcaggg ggatgtgcaa agcggctggt
     4021 cgacgacaaa aaccgatctc cctcaggtcg gcgatgacgc cattcggacg atggttgcag
     4081 cgcccgatcg cacgatttac atcggcaccg gcggggaatc gccgaatgta cacatctatc
     4141 aagccggaaa gctgcgcaaa ctggccaagc tgccgacgac gacttgggta agtgcgctcg
     4201 ccgttcgggc aacaccgacg ggaaatcatg ttcttgcagc gacggtagcg gatggtcgga
     4261 ttttcgacat cgcggaagac ggcaaagtcg agctatgggc caccgtcgaa gccgatcaca
     4321 tttgggcgat ccagcgagag ggcaaccaga cgatcgtcgc cacagccccc ggacgcctgc
     4381 ttttgatcca agatacggac tgtggtccga cggcaaagct tggtgccaat cagaaaacca
     4441 agagcaagat cctgttcgaa agtccggcgc ggcactttct ctcgctcgca aagggcgaac
     4501 ccggcgtcta ttacgttggc accagcgacg aagcgatcct gtatcgcatc acgctggaca
     4561 aagagggccg cgcgaccgct cgtgccaccc acgactttgc cggcaatgaa gtccgagccg
     4621 tcgctgccaa agacggaaaa attttcgtcg ccgtcaatga catgcagcgc ggtgataccc
     4681 agcttcgcgg caccaagctc accgtgcccg cagcgggaac tgcacccggc gtaaagccag
     4741 ccccaccgtc gggaaccacc gctccaccgt cgacaagccc ggtcgaaaag aaaggccgcg
     4801 gtggactgtt tcgcatcgac gaagccggtc gagtcgagca gctccacgcc atcagcgatg
     4861 gatttttcaa tgatttggcc gtcgatgaag atggcagcgt actcgcggct gcatcgacgc
     4921 ctggtggtcg aggccgggtc tatcgagccc tgccagatcg cacagtcatg actgcgcttg
     4981 agttcaaaga aagtgatgtg ctcgcgcttg cttggagcca gaccgagcgg atcgtcggca
     5041 ccggcagcgc cgctggcctg ttccaagttc agacgaagcc agcgacgggt gcgtcctatc
     5101 aaagcaaagt ccttgatgcg acggcgatcg ctcgctttgg tgcgctgcgt tttttggcgg
     5161 aaggcgtccg cgtcgaaaca cgcagcggca atgtgcaaaa accggatgct gcgtggagcg
     5221 cctggcagcc gctcgccaag gtcgcccggg tcgcgctgac tggtgaacag acgggggaaa
     5281 tcggatctcc gccgggtcgc tatctgcaag tccgctttct cttcgccgaa aagagcgtcc
     5341 tcaaagactt ctcggtctac tatcagccgc tcaaccagcg ccctcggctg accgacgtta
     5401 ccatcggtga agatccgctg tcgcggctcg ccaaaggggt aaagccaagc tatctgcggc
     5461 ccaagtcgcc gatcgtcaaa attcgctgga aagtcgaaaa tcccgacgag gacgacctaa
     5521 actaccgggt gttcatccgc cgcatccaaa gcacgcctgc ggcgccggga ggtccggcag
     5581 gtgacgtcgg ctttttgcgg atttcgggcg tcgatcccct gacccgcacc gagctggatt
     5641 tgaacaccga gacagtgggc gatggacttt atgaagtcaa agtgacggtc tccgatgagc
     5701 gcagcaacca aaccgacctc gcgctcacgc atgagctaag ctcgccgccg tttcttatcg
     5761 acaacaggcg acccgagtgg ctcgacgtgc gcttcgatgc gacaagccaa ctggtgtcgg
     5821 cgcggatcac agacagcgca tccccgatca ccgacttgtc gctatcgatc gacggtgggg
     5881 atttccaacc gctgaccagc cgagacggaa tcctcgatag cccaagcgaa gagatcgaat
     5941 acaagctggc gcggctttcg ccgggggcac atacgctgct gttgcgtggc agcgatgccg
     6001 ctgacaacgt cgcgacaacc cagctagtta tccaatcaaa atgaggactg gcctgtgata
     6061 tattcgtgct gcaaaagtga ctagtccttg gggagaagat tcctgtgcca agtgttagct
     6121 atgcgctccg gttcatctcg ggcaagtatc aaggtggcga atttccgctg cacaagggcc
     6181 gcgagatcgt tatcggtcga tcgagcgaac tggaaatggt cctcatcgag gacatggtgt
     6241 cgcgtcgcca tgcagccatc acgataaacg gtgatgagct gacgattgct gacgttggct
     6301 caaccaacgg cacgttcgtc aacggtgaga agatcaagcg ggctcggctc aaggaaggcg
     6361 accgcattct gatcggcacg tcgatcatga aggttgtcac cgtcgacagc aacatgaccg
     6421 aagccgaggc caagagccaa ctggaaaagc gcggtcagca gcagatgcaa aagacgtcgg
     6481 cagcgacgcg cgcaatgacc ggcaacatcg aggagattcc gctccccgac ttgctccagc
     6541 tgttttcgac gtcgaagcgc accggcgtgc tgactgtgaa tggaccgaac agctccacgg
     6601 gccagattta cctgcgcaaa ggccagctct actacgcttc tatcgacgat tcctacgaga
     6661 tgaagccaga gaaggccgcg tatcgaatcc tgacctggga caagggcact ttcgagctgg
     6721 actcgacgga ggaaaagaca ttcctcgaag agatcaaaga ctcgaccgaa ggcctgctga
     6781 tggaagcgat gcggatcgtc gacgaaatgc aacgtctcga tcccgatctg ccagcacgct
     6841 cggcccagtt ggtcattccc aagccgctgg ttccgtcgct gggtgagctg tccaaagacc
     6901 agctcgaggt cttccaagtc atcctgaacg gcgccaccac gcaaggcgta ctcgacaaga
     6961 gccgccaagc cgactgcgac gcagcccaag tgctggttga ccttatcaaa aaagagtacg
     7021 tcgtcgcccg tacctaaact ccctccgctt ccgctcgtcc tccgagcaac cggtttctca
     7081 acaagcagat tttcttttcc ccaaaatgat aagatgcagt ggtgaagacc atcgtcgctg
     7141 cattccatcc gactccccca agcgagcttc atcaaggact gctgcatcgc catgacattc
     7201 ggctgctcca tgtgagcacg ctgccggatc tggtcgatcg tttgggcaaa ggcgcagacc
     7261 tgtgtctgct cggaccgcag ctcgcggact gtggagccgc gacggcaagc cagacgctgc
     7321 ggagccatcg tcgagggcat ccgattccga tcatcttcat tcacaccgga accgcccacg
     7381 cggtggctct gccgcccgga ctgttcgatg aagtgattga gtggccggca cagcagagcc
     7441 acctgccgac actgctcgct cgctatctcg gcgttgcagc gcgcgcatcc gaacgcctgc
     7501 cgctcaaggt ccatgtctat cagggcacat ctgggaccgc gaccaaccat cctgatgcat
     7561 tcctcggttc gtcgatagac atcagcatgg aaggactgct gctgcggaca agccgcgacg
     7621 ttggcgtcgg tgacaagctc aatctgcgct tcactctccc aagccgcgtg caagcggtgg
     7681 tctgcttggg ccgagtgatt cgcatcgaca cgcgcaccca cgcaccggat ctcgcggtgg
     7741 cgctgcactt tgaatcgatg cccgaagaga cccgcagcgc gctggcagag ttcttcaagt
     7801 cggcaagtgg tcgcgcgttc cgctggaaga tcattcgcga cggtcagcga cagatcgtaa
     7861 agctgtcggg agtgctcagc gcggaagtcg atctgtcgcc gctcaagcag ctcaaagggg
     7921 aaatcgattt caacctgcgc gagttccggc gaatcagctc ggacagcatc cagacgtggc
     7981 tcgatcttgt gcgctctctg cgtggtgcgt cgaagattcg cctcgtcgag tgtcccattc
     8041 agtttgtgca gcaggccagc gcgatttcca acctgctcga tagcaccgaa gttgtctcgt
     8101 ttttcgctcc gtatgtctgt ccgcgctgcg ggctcgatga agaacagctc atcgacgtgc
     8161 gccgcgatct gtacagcctg caagggacac tggaacgcaa gcccccgtcg tttggctgcg
     8221 tccgctgcgg caccgcgatg atgtttgatg acatacccga gcggtacttc atgttcctgt
     8281 aaagcacgta atttcccaaa gcccagtcgg cccacaagcc aagggagaga gtcatgccca
     8341 cgcttgcgac cccggtgacc gttcgtttcg cggatcaaaa tggcaccgct gtgagtctcg
     8401 aaggagtgct cctttccgtc ggagaaaccg ccactacgga gctgcaaggg aatcaaaccc
     8461 gcaaaggact tcacctgctt ctgagcggcg atcgtgacta ctacgtcatg atttcgcagc
     8521 cgcttgcgcc cgatacccgg ctggcactga gcggtcaggt cggcaagagc gtgtcggcgc
     8581 tgtttaccgg tcgccctgaa gttcgcagac gactggcccg cctgattgaa tcgacccaag
     8641 atcccgaggc acagcccgga acgccggcag aaggtggccc aagccccgaa gcgtcgaccc
     8701 aaggcgcccc catcaatctg caagagggaa tcttcggagc tgcgccactg tttctgctgc
     8761 ccgagggccg gctcagtctg tcggcaaccg atccggttcc aacgagcatc gagcttactc
     8821 cggtatttgt tgcagctgcc cgctggattt cgtcgcggcg ccgctcgacg ttcgagtgtc
     8881 tgtttcctcc aagcgcattc catcccgatc tgccggttcg ccccgatcgt ctgtcggtag
     8941 cgcaagctgg cgtgctgctt gtgcagctgc gggacggact gtctgccgca gccgtgggag
     9001 gcgaaggcgc aaatgaaaat ccggtcgagg cggttcagct ccgttccgca gcgctcacca
     9061 tcttgtctca tcttgtcgcc acagttgcca aagatccttc gtttcgcacc gtcgcagacg
     9121 aagccgcaga gatcattttt tctctgatcg atgaggaaaa agacgatccg accgcgcgtc
     9181 cagcgctgcg cagccatgcc attcagcttt tgcaaatgcg cgcgccggca ctttcggcgg
     9241 cacacagaca gcgtgcgcaa gacctgttgc ggagtctgat tcgccaagcg cccccctata
     9301 cggagctccc gacggtgtgg cgatttgcca tgtgctcggc atgggacttc cacgaaggcg
     9361 aggtcgagct tttgcaaaag cgcttcggct ttaccaaagc cgagcccgaa aaaaatccgc
     9421 ccaagctgcc atttcccgtc gagcgatacc acgttgtgca ggctcctttt catggtccca
     9481 aaggcgagcc gatctacgtc tatgcacgca ccgcttcgcc caaagacgaa aacaccgaaa
     9541 tgggaagcga ggcattcgtc ggtgtcctta tcaatcggca tgcgcagctc ggttcgttcg
     9601 acatgcaagc gagtgcttcc gatgttcacc aagtcggcta caagctgatg atgaactcgc
     9661 agtgcgcggg acttacgacg cgctttgcga ttgccaagat gtttcctgaa gccgacatct
     9721 attcgtcgtg ggattcgacc tatttccgaa ccgatcccaa gagcggaaaa gtcaccgcat
     9781 cggaaggtct ggactgcttt gcagcactgc tcgaaggaat gagccgcggc gaatcgcacg
     9841 atgatctgag cgcccgcatc cagcaagcgc agtggcatca tgaacaagga cagcgcgacg
     9901 gatttgtgca gtttgttggt cccgcacacc cgctcgttat ttcgcgctac tcggatgtga
     9961 atcaggatgg gaaagcggac ttttacgacg gcttcctcga ctttaccctg aagaacattc
    10021 agaccgactt gcaagcgtcg gcaaccccgc gcgatccagg ggtcgcggca agtcagatcg
    10081 gtggcgatgc agccacggga ttgaactggg ccgcaggaag tctgaaccgc gtgacccagt
    10141 attccgatct gtggcgagga ctgcctggcg attccgagct tttttacatt ttctcgtcgg
    10201 gtggcttcta ttcccatctc gaacccccac aagatgtagc ggtcggacaa ggggcgcagg
    10261 aagtcgagct gggacggctt cccgcagtct gtcggtacat ccagaagtcg tatggaagcg
    10321 ccgacgaacc cggcctgtct gtcgaggtca tgttccacag tcacctttcc catgcggccc
    10381 cggaactgaa acgactgctg tgtgcagcgg aagccatgcg acgcggactg gatctgggaa
    10441 tgctgccgcc ggaaggaccg ctggcaacac agcttgccca gcgcggtgcc ctgcttttgc
    10501 tactcgcagg actgcttgag ttccctgccg accagaactt catcgacgga ctgtggagca
    10561 tggcgctcaa gctcctcaac ctgccgagca tttcacgatc actcgttcgc ggctgcatca
    10621 ccgatgccga tcacaacgca tcgaactact atggaagtcg ccgtggactg ctccagcttt
    10681 tgggtgatgg aacgaccgat ggcgcactga caaaatcgga cccgctcgcg tatgaacgac
    10741 tgcgcagcga cgatccgagc attggccgcg cgattccgct caagctttcc taaacgagca
    10801 agtcctcagc gcagatagtc agaaacccca gcagaatccc acgccgcctg taccgattct
    10861 gaatcagcaa atagatccgt cgaggcagcg atcgtagcgt ctgcgaggtc agtcgcgtct
    10921 gcatagcgat gcagataggt tgtgacggcc cgaaagtaga ggcgcgccgt tttcgccggt
    10981 ccaagctttg cagatatgag gaatcctaca tgcccaatat agccagcccg gaagcgtcgc
    11041 agcgaacgat cggtcagcac tggtgaagac gcaagacgca gctccactgg cactttgtcc
    11101 gtcgcaaaac gtgcttgttc ggtacgccgc ggatcttcca agtccgcgag cgctgccgtt
    11161 ccggtcaaag gtcgataaat cgaatcaccc acggtccaat tcggacgagc gattcccacc
    11221 gcatgctcgg aaagacaggc aaagatgttg gcgagtcctt catcgattgc accgctttct
    11281 ccttcggcgg ccagatccgc agtggcacga atcaccgcat gcgcatactc gtgtgcgacc
    11341 acatcgaatg cgcccccagc gggaagcaga gagggcggcg aacctgcacc gagcacaatg
    11401 cgctgaccat caaagagggc gaccggaaga ggcggaaaac gaacttcgct gtgcgttgcg
    11461 acgacgattc cgtgaccact gccatcccag ccaaagaact tgtgggtggt cgaaaagtag
    11521 tcccacaaaa gtgcaagatg cgcataagca gagaccgccg aaggaacatc ccagccatcc
    11581 actcgatcac tttgcaccgt ctttccaggg agtcgctctg ccgctttggc caccgtcgcc
    11641 cgcgctgatc cacgacggat gtcttccagc gaatagctgt cgccgcgttg ggtgatagac
    11701 agctcaaagg actccccaag cactccggtt cctctgccta tggtgctcat cggtacgtcg
    11761 gtagcgatca ccaagtcttc gcgccaaagc agactcagcg tgtgcgcaga aaggaacagc
    11821 gcgagccgga tcggcagatc cccatcttgt ccagatacct cgacgcgata gaccaaaaag
    11881 agctgctttt cgaccggaaa gtagtagagc ttgggagtct gcgcagaaaa tgcaacgagc
    11941 ggtcgctcag cagtcaggat acgcagtgac tcttgccgtg cagcctcggt gctgatctgc
    12001 ggaatcgccg cttcggcacg ccagctcagg agcggaacgg tatgcgcgac gattcgcttt
    12061 agcttgccag ctctgtcgag atgaccacgc aaaaagctgc cccagatcgt gacatcctga
    12121 ctcttttgcc gcagcgtgat cgcgcgcgag ccatcggatt ccgtcgtggt ttcgacactc
    12181 tcaaactgat cggaagacgg actaccaaac agcgaaggat agcgaagcaa aagtcggata
    12241 agcgacgagg ccaagcttcc agccgacggc agcgtcaccg caagggaatc ggcatgcgcc
    12301 accgtcttga gttcccgatg gtaacggagc cgaccaccga gcgtgcgctt tagctctccc
    12361 tcttgcgcag cctgtacttt cgcgagctgt ccatcatcaa gcagcggcgg cgacgcaacg
    12421 acctcggtat ttcgacaagc gcagagccca agaccccaaa gaccaaagag caacagaaac
    12481 aagcgtgtca tcggttctcc ttttttctgg acttggcgcc aatgcacaag ctgggccagg
    12541 gcacacagcg acggctgcgg aaaatcgcca aggccacctg tgtccggccc accggacgcc
    12601 gtccggtttc cagcgaagtc gcttgtcgcg ctggcctgac tgtggctatt gtccgcgcat
    12661 gctgcgtacc tcgtcgccat ttcagcgggt tcgttccacc tttcgtcggc atctccgtcc
    12721 gagcacgctc tgcgccagtt tttcgtcgct gctgcttttg acaagctgtg gtggtgaaga
    12781 cccaagcgcc ggaccgggtg cgttggcact gcatgtgctt cctgggcttt ccagtggcac
    12841 gatgaacgac tatttttcgc ttccgtttcc aaatgatctg cggctaaaaa cgcagccggc
    12901 cccgaccgcg cccgtgggtg agtcagagct gcgctcggga tatgatctga cgcagtttcc
    12961 gcagcccgca ggtcaacccg gaaaatacgt cgcattccta aacggaaaaa ttccaggtgc
    13021 aggtccgggc gctgcgatct acttccgctt tgatgcgccg ctggatacaa ccacactgcc
    13081 ggctgcggaa cgcagcacag atgacgattc gaccgcgttt gtggtcgacg tgaccgaagg
    13141 ttcgccaacc tatctgaaac ggactcccat cctggcaaca ttccatcgcg aagggaatcg
    13201 ctacatcggt gacaactggg tgtccctgcg gcctgtgccg ggtctcccgc tgcgcgaacg
    13261 cacgacgtac atcgcggtgc tcaccaaggg agttcatgcc gatgctggcg gcgagattcg
    13321 gccagacatt ccgctgctgg ccatgatgtc agtcacccag ccgatgaatg aaccgtacag
    13381 aagcgcctgg ccgcgctatg caccactgcg caactggatt gccgcgaccg ggctgcgcga
    13441 caaggtgggt gcggcgacgg tatttacgac gcaggatgcg acatcgatga tggggaagct
    13501 gcggcaagcg gtctatgatc agacagcggt gccgactccc aaagcggtca cgtacctgcg
    13561 cagccacgac ggaatggccg atctgtatac cggcacattc gcttcgccga acttccagag
    13621 tggaacgccc ccatactgga gcgaaggcgg gggtctggat ctggatgcgc gcgggattcc
    13681 caaagtagtg cgcacggaaa atctgcgctt tgcgctgtcg attcccaatg ccgagatgcc
    13741 ccccgatgga tggccggtga ttctgtacgc gcacggaacg ggcggtgact accagacctt
    13801 catccgcgag cagctcgacc tgcgcgcctc gttgattgag caagcaggac ggccaccggt
    13861 tcggatggcg atgatcggaa tcgatcaggt tctgcatggg ccgcgcgatc cgtcctcttc
    13921 caatccggaa attacgttct ttaacttgca gaacctggaa gccgcgcgcg aaaacgtgaa
    13981 acaaggtgca gtagatgact ttcaactgct gcgtctcgtc gagggaatgg acattcccaa
    14041 agcacccacc acaggtcgtc cgatccggtt tgacaaaaag cggatctact ttatgggaca
    14101 ctcccaaggg ggtctgaccg gaccgctgtt tttggcggcg gaaccgaaag taccggcggg
    14161 aattttgtca ggtgcaggat cggtgctgat tttgtccctg ctcaacaaga cggaaccgaa
    14221 caacatcgcc gagcttgtcg agagtctgct gcaagagccg ccgctcgtcg atcatccgct
    14281 gctcaacttg ctgcaagcgt actttgagtc gtcggacccc aacaactacg gcaagctgta
    14341 tttccgcgag ccccccaaag gaatggcgcc aaagagtatc ttccagtcgc ttggtctttg
    14401 ggatcacttt acgccggttc ctaacattgc cgcgttcgcg cttgccatgg gggtcactcc
    14461 cgtcgagccg cggctgtatg atctgcccta tctgtcgctc accgatctgc gctggaaaaa
    14521 tggaaaagtg gccggcaatg tcgccgatgg aaaggccacc gccgttctga agcagtatgt
    14581 ggctccgccg cgggacgatg gacacttcgt gatcttcgat ctgtgggcgg cacgtcatga
    14641 ctggtcgcgc ttcctggcgt cactggcgca aggcggtctg tcggtgctgg aatagctcga
    14701 tgagtaggaa caaacagctc gtccttttgc accgtcccac ggggcgcgtt tccatcgatc
    14761 actttgaagt ccgtgagata ccgctgcgcc cgcccagtga cggcgagctg ctgatcaaga
    14821 cgctctatct gtctatcgat ccgacgaacc gcgtctggat gagcgaccgc ccacagtaca
    14881 tgcctcccgt cgagatcggc gaagtgatgc gcggttttgg tttgggtgaa gtcatcgatt
    14941 cacgccatcc ccaatttgcg cgtggtgatc tggtgacagg actgcttggc tggcagaccc
    15001 actgctactc cgatgcccaa ggactgcgtc cccttcagaa aattccgacg gacacgggga
    15061 ttccgccccg cacctttttg ggagcactcg gactgaccgg tggactcact gcgtactttg
    15121 gcatgatctc gctgggagat ccgaaagcgg gtgaaaccgt cgtcgtttcg gcagccgccg
    15181 ggtcggtcgg ttcgctcgcc ggacagcttg ccaaacttcg cggcgcgcgc gtcatcggca
    15241 tcgctggggg ccacgaaaag tgcagctacg tcgtcggtga gcttggtttt gacgcctgca
    15301 tcgactacca aaaccaggat gttggcacgg agcttgagcg gctctgtccg cagggcattt
    15361 ccgtctactt tgacaacgtc gggggatcga ttctggaagc ggtgctggcc cgtttggcgc
    15421 tgcgcgcgcg cgtggtgctc tgcggcatga tctcacagta caacgacgaa caaagatgga
    15481 ctttgccgcg caactttgac gcgattttgt atcagcgtgc gcgcgtcgaa ggcttcatcg
    15541 tcagcgattt tcgcccgcag tttcccaaag cctatgcaga gctgattcca ctgtggcgta
    15601 gcggtcagct cacatatcgc gaacacttgg tgcacggact ggaaaacgcc cccgccgcgg
    15661 tcaacatgct gtttgatgga aaaaaccacg gcaagctgat cttgcaggtg gccaccgacg
    15721 gcacatcatc ataagcatag gaacacctga tgtcggcaaa acatgttgtt gtaaccgggg
    15781 cctcttccgg aatcggcgct gcgctggtca cggagtttgt tcgctgtggc gcgcgcgtca
    15841 ccatggtggc gcgtcgccaa gccgagatgg aggcgctcgc ttcccagcgg ccaggacaga
    15901 cgcagttctt cgtcgtcgat ctgagccgaa cagagaccgc gctcgattgg atcgcgaaag
    15961 cggaagcgac gtttggcccg attgacgtac tgattcacaa tgcaggtgtg cagcttgtcg
    16021 agcctgcgga agaacacgat cccgaagcgc tcctggcgat gctgcggctg aaccttttga
    16081 caccgcttgc aatcacgcga caggtcctgc ccagcatggt ggcgcgcgga gacggaacga
    16141 tcgtcgacat tgcgtcggtt gccgggcttg cgccaacccc cggaatgtgg ggctataacg
    16201 cgagcaaagc aggtcttggc gccgcatcgg agagcctgcg cggtgagctt cgccaaagcg
    16261 gtgttcatgt tgtgaccgtg tatccaggtc cggtcgatac gccgatggcg cgcgccggat
    16321 atgcggccta tcctgaaaac cccgcgctgc gcgtgctacc tgaaggaaga ccgagcgagc
    16381 ttgcgcggct gattcgcgaa gccgtcgatc tccgacgccc gcgcgtcgtc tatccgtcgt
    16441 tttatgcgct gctgcgctac attccgacgg tggcgcgctg gttcctggat cgaatgacgc
    16501 ccagaccgtt ttcgcgcaag gatcgaacct gatgcgggtt tctcgcgatg cacgctttga
    16561 tcgcgaacga gcggagtttg cactccggca ctgctgcgag gactgcggtc tgtttgtcgc
    16621 ccagcgaaac ctctgtgcac atgagtggcc gacggaagaa catcgccgcg ccgactatga
    16681 aaaaccgacc gcggacctgc ttttttgcaa agagttcgag ctggcctaag ccaccaaaac
    16741 aactactgcg cttggtttag gtaggaaatc agcacgaggc caggtccgcc aagtaccacg
    16801 tcggggctgt cgttgccgtc gagcaaaccc acgtcaatcg tggttgcgtt gtccggaagc
    16861 gtcaaaagca ccgatggcgc agcaaagctt ccgtctgctt ggttggcgag ccatgacagc
    16921 tggctcttgc ggctgatgat tacgtcggcc aggccatcgc gatcgatgtc accgatggac
    16981 agcgcccgaa caggatcgct gccaagctgg gtcgaaaggc tctgcgacaa agactcatcg
    17041 cgtgcaaggg atgtcgccgt agcaagccac accgctgcac tgccatcttt gccaacgctt
    17101 accaaatcga cgctgtcatt gtcatccagc gtccccgcag agatcagcaa aagcggcccc
    17161 gtcgaagcag agaccgtcag ccgcgatgtc gccgcactcc cgccatcagg cagccgatat
    17221 gcgcgcagaa aaggaccgct tccgccctcc cctgcaaccg ccagcagatt gccagcgccg
    17281 cgttttcccg ccgccagcgc cgtcactgat gcgtagtcga gactggcttc gatgtcgctg
    17341 taacgaagct cacccagcgg agactgctgc agcgcaaagc ccgccccact ggggatcagc
    17401 cgcaaaaatg ctgtctccgt gcttccaaag agcgccgtac tcgggacagt aaatgccatc
    17461 gaaagaggaa cggatgcggc cttcgtgagc accggcggtt ggtatgtcca gtcgccgaag
    17521 gttcggtcgg tgacgccttg ttcgagcgcg atgacatgac gactgaccac cgacagccag
    17581 acaatcttcg ccgagcgcga gacatcagga ccgaagctcg gtggcacata gagacgcagc
    17641 gtccccgccg tctgttgatt gggcgtcacc actttgatcg gcacaggtcc cggcaaaacc
    17701 gtcggccaca gctcttgcgg ctttgccatc gcatgccaca gcccatcccc cataagccgc
    17761 aaaggcaccc gcttttcgtc aagtcccgcc ggggacagga gcacatcaac cgattccggc
    17821 gagcccatca cctgcacgac cagctcaccg cccaatagag gcagccgccg actcacaagc
    17881 cgcgccggcc cctccacctg cgtcacttga cactcggcaa gatccggctg cctttcacag
    17941 aggttttcga ccaagcagcc acttagcaaa ggcacggcca aaacggccac tgacaaacgg
    18001 ttcattgcgc ggctcctagg gatgcgctgc gacggtggag cttgtttctg ccggccaaaa
    18061 caacgtcagg ccagccccaa tgagaagcgc gctgcccagc ccgtaccccg caccgtaaaa
    18121 ccacttggtg ttccacgcac aggcctgcgg tacggaagtg ggggcacagc gtatgtcggt
    18181 ccgatgcccg tcgagtccat gaaacgcgcc cgccgtcgcg aagacagcca aagacaccgc
    18241 accgagcact ccggcggcaa ccttccgtcg cgcggacaac cgatcaaagt agctcccttc
    18301 ggaaccacca tgtgaccccg cgagactcac aggcgattcg accagcgcag gctttggcaa
    18361 agccggctgg ttcatcgcca gcgtcagctc aggcgtccga atctcgaaaa acagcttgtc
    18421 ggccagttcg gtgatgcgcg tgacgagcag ctcgcctttg cagcccatgc aatcgagcgt
    18481 cgcttgccgg ggccagtggc gactcgcatc gtacagaaga ccggtcaggt acaagccatc
    18541 ccccgtcgct gcggtacgct gcgcggcgat gctcagcaca aacttggccc ccgctttggc
    18601 ggccagacgg tcgaggcact cctgtgacgt acactgttgc tcgcccggac caagcttcgg
    18661 cgtcggcaga agcagctcgc cgttgcgggc caggtgatca cgcagacgcg actgaagcac
    18721 cccgtcttgg cggttgtcac ggaacacacc gatgaggaca accggttcgt ccgcagccga
    18781 gaccagcgac ggccacgagc acaaaaacag accaaacaaa tagatgcgcc gcagaacaga
    18841 cgccacgccg gatcgcgaca cggtcatccc ctgctagctc tgtttggaac gatgaaggta
    18901 tttcagacgc agatcgtaca gcgcggtctc gacgagacga tcttcatcag cggtcaaatt
    18961 gccacgggtt ttttcgcgca gcatcccgat gatgtcgatc gtttgtttgg ccatcgcgag
    19021 atcgatgggg gtgtcgcgca gctccgcggg agccccctct tcgtgctcgc ccatgtgaag
    19081 aagcgccgac gccgcaagcg acatcacgaa ggtgacaaag tcaatctcgg gttcctgaga
    19141 agtcgcggac atgatgccta gctccggcta ctcgtcgtcg tcatccagat cgtcgtcgtc
    19201 gtcgtcatcg tcgtcgtcga tcaaatcgga gtccagatca tccgaatcaa ggtccatatc
    19261 gcgacgcaca gcaggcgccg cagtgatgcg aacctgagga cgacggggtg gcggacgctc
    19321 aatcggttct tccagcggaa tcgacgctgt cgccttgtct gcgacgtcgg gcaaggtctg
    19381 aagatacgct tgcagctgct tatcggacac ttcgccccga cgcagacgac gctctaggat
    19441 acgaacatcc cacaaaatgg gatcgttact caggtcagcc atgatggtcc tcatatagag
    19501 gcgaacgcgc ggcttttgtc aactcaaact cccatcgcag caagcgcgcg acgcttcctt
    19561 ccgttttcgc gcaagttcta cgatgatgcg ggcagccaag gagacatttt cgtgcaaagt
    19621 tctgctcgtc ctctcgactg tcgccattac atgccgctgg gtgctgccgg ggaagaggcg
    19681 gcctcaaacg gcctgtcaaa gcttgcgctc cagctcgtcg gctcggaaat tctcaaaatc
    19741 gcggcggcgg tgcgggcgct gcaagcccaa ggtcacgcgg tctgcaactt gaccgtcggg
    19801 gactttctgc cgaagcaatt tccaattccg cccaagctgc aaagcgccgt catccgggcg
    19861 tatgagctgc gcgagaccaa ctacccaccg tcggacggta tgccggcgat gcgggaagcc
    19921 gtgacggagc tgtatcggcg gacgctcggg cttgattatc cggtcgaaag cgtcgcgatc
    19981 ttcgggggcg cgcgtccggc gatttacggc acgtatcgcg cgatcatcga tcccggcgag
    20041 cgcgtgattt attcgacgcc gtcgtggaac aacaaccact acgtgcggct tgcagatgcg
    20101 attccggtcg agtgtgaaac atcgccggaa gagggcttta tgccgacggc caagtcgctg
    20161 cgtccgcttc tggctggcgc gcggctgctt tgtctaaaca gtccgctcaa tccgacggga
    20221 acggtgatcg acaaagacga actgcggcgc atctgcgagc tggtgcttga tgaaaatcag
    20281 cgacggaaac gcagcggtga gcggtcgctt tatgtgatgt atgaccaggt ttattacgcg
    20341 ctgacgttcg gacaggcgaa acaccacacg ccgcccgagc tgtgtccgga catggcggcc
    20401 tacacggtgt ttgtcgacgc gatcagcaag tcgctgtgtg cgacgggtct gcgcgtcggt
    20461 tgggccgtgg caccacctta tgtgatgggc cggatgcgtg acattttggg ccacgtcgga
    20521 gcctgggcgc ctcgtcctga gcaagtggcc acggcagaac ttttgcgtga ccccgacgga
    20581 cttgcgacgt tccagaccca gatgatggcg gaagtcgagc agcgcctgtc gctcctttac
    20641 gacggaattt cgcggctcgc agcggaaggt ctgccagtcc gcgcgattcc accgcaaggc
    20701 gcgatttatc tgtcggtgca gttcaacctg catggacgca agctacccga cggaacggtg
    20761 gtcgaaacca acgaacaagt gcgaaattat ctgctccacg cggcgggcat ggcggtggtg
    20821 ccgttccagg cgttcggaat gcgcgccgag acgggctgga tgcggctttc ggtcggtgcg
    20881 gtcagcatcg acgatgtgac caagctgttg ccgcgcctgc gtgaggctct gttcgctgtc
    20941 gcgtagaatg ctgggcatga atcggtcgaa gccgtcactg cggcccacct ttgcactgct
    21001 tgcgtcggtg ggatttctcc ttggggctgg ctgctcggtg ccaaccgaca agctgccgct
    21061 tgggccgatt ccgctcgtgc tggaaaatgg gctcctctat gcgcgcggac agcttttgcc
    21121 gacgcgcgac gagcgctgcg gtcagacgcc ggaagaaggg gcgccgcccg gctcgggtct
    21181 cgatgcgatt ctgattgata ctgcgacgcc actgacggcg ctggtgccac aagaagccga
    21241 tacgccgcgg cagtttcagc acggccagtt cagcttatac gccgcgctcg ccgggggaaa
    21301 aagtggagcg acgcagctcc tgcgctgcga tgtcccggtg gtgcgcagcg atctgtcggc
    21361 acgcgatttt ggactgcgct gggacagttc accgaaactg ctgctgggtg gtgtgatcgg
    21421 ggctgaccag cttttgaact actcgctgga gctgcgactt gcgaccgacc gtccgacgat
    21481 ccagatttcg cgcggtgact tggctccgtg gtgccgcgtc gaccgcgcag tgattccgtt
    21541 caagccgtta ggtggcgagc tcgcggtgca gctttcagac accgtgattc agtatccagc
    21601 gacgcgaatc accgtcggag cgtgtgtgga accgctgagt gatccgctgt ctggaccgcg
    21661 gcccgtcgac tgcatcagcg atgcggatgt cgatgcagcg cttcagcgag tgctgctcgc
    21721 gattcggacg ctggaaagcc agcagccgcg cgacgaagag cagctgaccc ggctcgccag
    21781 tgacgaagcg ctgctccgcg ggctcaagtt aggtcagtgt accgacgaca aaagtacgca
    21841 gctcggcgat cttgcggaca atcagcggct gcgacgagcg ccgtatgagg tgtcgggcac
    21901 aaacatgcgc tttttggtgt cgacggcgct tcctgagctg gttctgaccc aaacggcgtg
    21961 tcagcggctg ggcggcaaga cgtgtagctg cgacgaaaaa aaccgtgttc cacttctgtt
    22021 gccggggctg aacgccaagc ggcagagcga tggaagtgtg gtcgattccc ccgactacgg
    22081 ctgtcggatt cagcttggct acagtggcca tgcggcgctc gcgcttatgg ccaagcagcg
    22141 gcagctttcg ccttgtgacg aactggcgcg cagtcgacgc cagcgctggg ccatgagtcc
    22201 agcgccagcg ggcgccacga tgcaaaacat ctgtcagcgc tcgctctgtc tacgaaacct
    22261 gggacgcgac ccggcgcagc tgaatcggcg ctgcgggtac accggactcg attccgactt
    22321 ggcgtgcaat gacaagctgt cgcccgtggc agcagcggtc gagctgggcg gacccattga
    22381 cgacaccacc accgacacac tcagcgcggt ggtcgtgccc gattcggcgg cacttgtgca
    22441 gtcagccaat gccgacgtgc gcaacctgtc ggcccaactc gacggagtga tcggcgtgtc
    22501 gctgctgcgc aggctgtaca cgacgattga ctatccgcag ggccgaatcg agcttttgtg
    22561 ccgctgtaac gatccgctgg cgacgcaaaa aaccgtctgc caagcctatc gtggcatcac
    22621 gtattacacc gccgatgcct gcgtcgaaag cgacacgctg accgtgccga gcgacctggc
    22681 ccgacttcgc tgcgacggct gacctcgcgt tttctgcccc cgctgtggta tggcagtcac
    22741 gtcgcatgat gaaacttgct tgcactccgt gttctgcacc ggctggccga ctggcctttt
    22801 tcgcaacgct tttgctttct gcgactgcgt cagcgcagcc agcggacttg gtgatccaaa
    22861 aagcccatgt cttgacgatg gacggagcgc gtcccacggc aaccaccgtc gctgtgcgcg
    22921 atcgccgcat cgtctatgtc ggggatgaag ctggtgtgtc ccagttcatc ggtccacgca
    22981 cccgcgtcgt ctccagttcc ggcagtcccg acatgacgct tttgcccggt ttgcaggatg
    23041 cgcatgccca cttggttggt ttgggcatgt cgctcgccgc gctcgatctg cgaggtctgt
    23101 cgtcggtaga agcgatctcg caggccgtga ccaaagccgc gcaggaacac agcgatcgcg
    23161 gacagtttgt gatcggcaga ggctgggacc aaaaccgctt ttcgccaccg aattttcccg
    23221 atcgagcaca gctcaagcgc ctggatgaag catcggcggg acatccggtc tggctgcgac
    23281 gcatcgacgg acacgccggc tgggccaaca gtgcagcgct ggcgctcgct gggattggtc
    23341 gcaagaccgt ggacgtcgcg ggcgggcgca tcctgcgcga tgacagcggc gaaccgaccg
    23401 gcgtgctcat cgacaacgcc atggatctgg tcgaacgagt cctgccgcag ccgtcgggca
    23461 aagagatcga agcggcgatc gtccgcggca gcgggtacgt cgtcgcgcgt gggctgactg
    23521 cggtgcacga catggggatt ggtccggatg cggtcgctgc atatcggcgt ctcgccgagc
    23581 aaggccgctt gccgctgcgg gtctatgcct acgccgaaga tccggtgccc tcgaaacttc
    23641 agcagcttcc gcactcgctg gcctaccggg ccgagctgga tcgcttggcg cagcgactcg
    23701 ggcccccgga tcaaagcgga ctttttaccc tgcgcgggat caagctgttt ttggacggag
    23761 cgctcggatc acgcggcgca gcgctcgagc agccgtacag cgacgatccg caaaaccgcg
    23821 gactgctttt gacatcgccc gatcacatcg aactgatggc acgctgggcg ctggtccacg
    23881 gctatcagct cgcgacgcat gcgataggcg atcgcgcggt caacttggtg ctcgatgcgt
    23941 atcgacgcgc cggtgtcagc gcatcccgca atgcccgctt ccggattgaa cacacacagg
    24001 tcgtcagcga cgaagtgatc agccaaaggc agctccagtc gcttggggtc atcgcgtccg
    24061 tgcagccgca gcatgcgcca agtgatgcac cgtgggcgac gcagcggctc ggtcccgatc
    24121 ggatcaagct ggcctatgcg tatcgggcgc tgctcgattc gggggcacac cttgccggcg
    24181 gttcggactt ccccgtcgaa gaagccgatc cacggctcgc gctcgatgcc gcgctgtgga
    24241 aaacgcaagc cggaaagccg ggcagcaaac ctccagccga tgcactttcc gtcggggaat
    24301 ccgtgcgact tttgaccagc gatgccgctt atgccgcgtt tgccgaagat cggctcggca
    24361 gaatttcggt tggcatgctc gcggacttga cgctgatcga aggcaagctg tcgctcgacg
    24421 acaaagagcc ggttccaagc gatctgccgc gccgcaaagt cctgcttacg gtggtagatg
    24481 gtgtggtggt ctacgatggc ctgggccaaa atcaagccca ccgtccacgc cgtcgcggac
    24541 ggcttgtccg ccaccgctag tccacagcaa cgggccggcg cgatatgatg tcggctatgc
    24601 ctgcaagtct tatgatcgcc accgaggagg ggctcaccgt ggagctgcac ctgagcagcc
    24661 agccggtgag cgtgggacga gccaaagaca acaccttgcg catcgacgat cgccgcatgt
    24721 cgcgccacca tttggtgttt cgccgtcgag aagacgcaag ctatgaagtt gccgatctgg
    24781 gctcgtataa cgggacgctg ctcaatgggc gacggattcg ccaagaatcg ctcaaagccc
    24841 aagacatcat tcgctgcgcc ggattgcaga tccagctcat cgaaaaagag agcgccgggg
    24901 atgccgtcga gccgtcgctg atgacggtga caaaccaagc gcttgtcgaa gcccgctcgc
    24961 agatcaaaca aatgatcgac gaacaggcga tgctgcgacg cgaagtcggt ctggcgcagg
    25021 aatccgaagc gcgcgccaag cggctgcgcg atgaagcgca ggacgaagtc gagcggctgc
    25081 acgacgtgat cgccaagctg ggccaagaca aagagtcgct cgctgccaaa gtcgaagagc
    25141 tgggcaaaga gctgcgcgaa cggctggcga cgaagaccga cccgcgcacg gacgagctta
    25201 cggccaagct aagcgaagcg cagaagcagg tcgaaaaaca caaagcgcgc atcgccgaac
    25261 tggaagaaaa agacgctgcc cgcgtcgcca gtgaaggccc gctgcgcaag gaaatcgacc
    25321 ggctcgcgga catcctgaaa aagcgcgatc agcgagagct ggagctgcaa gccgcggtaa
    25381 agcccgcctt gattcgcatc gccgagctga cccgcgagct ggaaaacacc cgcatcgaac
    25441 tggcgcacag ccaagccgat ctggaagaca tcaaaaaggc cctgctcggc aagcgctaaa
    25501 ccaccgattc cccctgccct ttccctgtag acgcgctctg cctggttggc ggacactccg
    25561 cccatgcgcg cccccttttg gctcggttcc gtcgtgctgt ttttcgtcgc tggttgcggc
    25621 gaagattccc ccctagaaaa acccgaagcg ctgtcgtttc cggcaagctt tcgtttcgga
    25681 accgcgaccg ccggttttca ggtcgaaatg ggctgtccga aaacggccaa agcagactgc
    25741 gagtttccgt cggactggac cgattacgtc acgcgacccg agcttgtcgc gcagaaaaac
    25801 ctgtacctaa gcggcgatcc accgtcgcgc ggtcccggct tctttgaact ttatccgcag
    25861 gacatcgcgc gcgccaaaca ggaactgcat agcaatgcgc tgcggctgtc gatcgaatgg
    25921 ggtcgcgtat ttcccaatcc caccgacgga atcaatggct acgaagcact gcgcaaagcc
    25981 gccgatccca aagcgctcgc gttttatcat ggtgtgtttt cggcgcttcg ccaaaacgga
    26041 atcaagccgc ttgcgacggt gattcactac gttctgccgg cgtggattca cgacgcctac
    26101 ggctgccaca caaactttgc cggatgcaaa aatcgcggct ggctggatca cgatcgcatc
    26161 ctcagcgaag cggccaaata cgccggtttt ttgggtcggg aatttggcgc ggaagtcgat
    26221 gactgggcga cggaaaacga acccttcaca gccgtcatca ttgccggcta tctgttccaa
    26281 accgagcagc gcacgcaccc tcccgcggtg gttctggaat cggatgctgc gcgcgcagtc
    26341 ttgaatgcgg aaattgaagg ccacgcgcgg atgtacgatg cgttaaaagc cgcagatacc
    26401 gcggatgccg acggagacgg ccaagctgca cgggtcggtt tggtctacaa catggaagcg
    26461 gttgcgcccg ccgatccaca aaatgagatc gatgtaagcg gcgccaaaca cttcgactat
    26521 ctgctcaatc gcgtcttcct aaacgccacc atccggggtg atctggatgc aaacctcgac
    26581 gggaaaacgg ttcacagaac ggacttggcg ggtcgaatgg acttcctggg cgtcaactat
    26641 tacgcccgca acgtggtgca agggagtccc aagtcgtttc tgccgcagct ctctccgctg
    26701 ctcaatttca acatactcac cgtcgagtac gactggaatt atgcacgcgg tctgtacgaa
    26761 gtgctcgact ctgtgaaagg ctttgggatt ccgcttgtgg tgacagagac cggcgtggaa
    26821 gatgccagcg attccggcag tgctgccgcc tgggttgtcc gatccctcac ctgggtttcg
    26881 cgcgccatcg cagatggaat tcccgtcgag ggatactatt actggacgct gatggacaac
    26941 tacgaatgga accacggcat gaatgtgaaa atgggtctgt acgcagttga tccaaatgat
    27001 ccccaaaaga cccgcagggc ccgccgcgca gtacctatct atgggcagat tgcgcaagcc
    27061 ggacagattc ccaaagagct gacggatcaa tataaagagc cgcgagatcc cccgcacagc
    27121 gacggtggaa agaccccaac agctatccga atcggggccg cggcaaaagc ccgctaagca
    27181 gctctgctat cagcagttcc tcatcacatc cagcgagccg ctcgatccca tcttcattga
    27241 cccagcgacg aatttgtagc gttccaatcc gcgtctgagt cagcacgccg actcgcgcaa
    27301 aaccgcgatc aatgcgcgga cgcacgctgg ccattccttg ttcaaaccac gcatcgtttc
    27361 gccctgacgg tgcgcgggta tcgacgagca gcgcccggcc tctgcgacct gtcctatcaa
    27421 tggccgcgat cactttttca tacgacccca aaagcgccga cagatccgga tacgcttggg
    27481 cagtccgctt gagccgaatc agccgcgcct cattggtcaa aagcacgcaa gcgtactcgt
    27541 cgctgtagag cgattttgca tcccctgacc ctgacggcgg cgacaaactc acaggagatt
    27601 gagtgtacct ttggcgaagg gatagcccca gccgaaaatg caaacggtgc gcagctattc
    27661 ggatactgca cgcgcctgcg cgatgagcga cttgataaac gcttcctcat cttgcggatc
    27721 ttgtttggga ggcgggcctt ccaagtcgcg tagcaccgtc agagaatccg gtatgtcttc
    27781 caaaaatcgg ctgggatttt tgggccagcc accgcgcagc tcggatcgcg aaatgtagag
    27841 cttctgccgt gcgcgggtga ttcctacgta acagagcctt cgctcttcgg aaatgtcgga
    27901 agacgggtct tccccgtcgc tgtttccatc cgaatgacgc tgcgccgcaa tcgtatgatc
    27961 ggcttcacgc ggagccagcg cccgcttgtg cggcaggatt tcttcttcca gtccgacaaa
    28021 aaacaccact ttccattcca gacctttggc accatgcagc gtcgacagcg tgacttcatc
    28081 ccgcagcgag tcatcgacat cttcatcctg cgatgaaatc gaaagccgat tcaggtacga
    28141 cagcatgtca aagtccggtc cggctttttc ggcgtagcgc tgcatgctgg ccaaaaactc
    28201 gctcaggttg cgcagccgtc gctctgcttg cagcatcgtc ggtccagcgc gcaccagatc
    28261 atcgtggacg tttagctcac gcagcagctc ttctgccacg tctttgagct tttccgcgcc
    28321 aacctgggca tacatttttt gatacttttg cagcgtgcgg acaaagcgaa acagcgacga
    28381 catcacccgc ggtggcagcg cgtcatgctg cggcacttgg gaatcgtcgg cgaacagatc
    28441 gagcgatccg gtctgggttc cgcacagatc gcgcagcgct tcccacagcg acgtatctgg
    28501 tttgcgacgc cgcgcttttt ggtgaatctg cgacagcgct tccatcgcct gcgggccaat
    28561 tccgcgcggt ggatagttga tgacccgccg cagcgaaagc tcgtcgtgcg gataggtcag
    28621 catcttcaaa taggccatcg cgtctttgac ctctttgcga tcaaaaaacg cctgtcctcc
    28681 gatgacgtga tagtcgattc gcgcttgccg cagcgcttgt tcaatcggtt caagctgacg
    28741 cgaagtccga aacagaatcg cgatgtcgcg cagatcaaac tttccggaaa tcaccagcga
    28801 atcgatctcg gatgcgacaa actccgcttc ggcgtcttca tcgggagcga tgacaagctg
    28861 aatcggctcg ccatcatcgc gctgcgtcca cagcgtcttg gcatgtcgcg cgctgttttt
    28921 cgcaatcacc gcgttcgccg ctttgaggat tcctcccgtc gagcgatagt tttcttccag
    28981 cttgatgacc tgcgcaccag gaaactgttt ttcaaactgg aggatgttgc gactgtccgc
    29041 gccgcgccac gcatagatcg actgatcatc atcgccgacg accgccaggt ttctgtgctg
    29101 ctgacagagt atccgcagaa gtgaaagctg acagtggttt gtgtcctgat attcgtcgat
    29161 catcaggtaa cggaatcggc tctgccagcg cttctgtacc cgctcgtcct cgctcaaaag
    29221 tcgcagcgtt tcgacgagaa gatcgtcaaa atcaagcgca tgaagcgccc gcattttttc
    29281 gacgtagcgc ggatagactt cggcagtaaa gccgtcgtac tcgctgacaa agcgggctct
    29341 gcgcacctgc gtaataaagt ccgtcgccgt cagaccggcg tttttggcac ggcttatgcg
    29401 gctctggatc agctttacat ccagcttctt tccgtcgaga tgcgcgtggc gcagaatttc
    29461 gcgaatcaca ccgagctgat ccgatgtgtc atagaccgaa aaacccgtcg gcatgccaag
    29521 cgccgcttgt tcctgtttta ggatcaaaag accgagcgcg tggaacgtcg acagcatcac
    29581 gccgcgcgcg acccctccga cacacaagcg atccactcgt tcgcgcatct cgtcggcggc
    29641 tttgttggta aagctcaccg ccaagatgga ttcgggacgg acgcctttat ccaacagatg
    29701 cgcaatccga taggtgataa cccgcgtctt gcccgaacct gcgccggcca ggaccaaaag
    29761 cggtccctcg gtggtgatca ccgctttctg ttgcggtggg tttaagtcgg cgagccgaat
    29821 ccccatgagg cggaacggaa acacaagttt gcgtgtacgg ccacattttt cggctatagt
    29881 ccgccgccat gttttcctcg atcgcaaggc atccgtttcg ttttgtcacc ttgggtggct
    29941 ttctttccgt cggacttttg gtgtgggcgc tgcctccgca agggggcgca aacgcagccg
    30001 gaaaaaccaa aacagccatg gcaaccccgg cagaaccgaa gctgacctat ccacccacca
    30061 agacggttga tcaggtcgac gagtatcacg gcgaaaaggt caaagatccc tatcgctggc
    30121 tcgagtcgct cgattccgag gagacgcgac agtgggtcga agcgcaaaac aaagtgacgt
    30181 tttcgtatct ggaaaaaatt ccgggtcgcg acaagctgaa gcagcggctc accgagctgt
    30241 ggaactttga aaagtactcg gcccccttcc gcgaagggaa tcgctacttc tacagctaca
    30301 acagcggtct acagaaccag agcgtgctgt atacgctgac gtctctcgat gcgcagccgc
    30361 agcttctgtt ggacccaaat acgctggcca aagatggcac tgtcgcgctc gccggaatgt
    30421 cgctgacgca cgacggaaac ctgatggcgt atggtctgtc agccgctgga tcggactggc
    30481 aggaatggcg ggtccgtgat gtccaaaccg gcaaggatct gcccgacgtc atcaagtgga
    30541 tcaagttttc gggtgcggcc tggacacgcg acaacaaggg atttttctac ggacgctttg
    30601 acgagccgaa gaacaccggc aagagcctcg aagacgtaaa ctttttccag aagctgtact
    30661 accaccgctt gggaacgccg caggcccaag acacgctggt ctatgaagac aagcagcaca
    30721 aagagtggca gttcgctcca caggtttcgg acgacggaaa atacctgatt gtcacggtgg
    30781 ccaaaggaac cgatgacaag taccgaatcc tgtaccaaga gctggacaaa ccaggcgcga
    30841 aatttgtcga gcttatcaac aacttcgacg cggagtacac attcatcgac aacgatgggc
    30901 cggtgttttt cttcaaaacc gacctcaagg cgcccaaagc aaagatcatt gcgatcgatg
    30961 tccggaagcc gaccgagcgg aaggaactga ttcctgaatc gcaggacaca ctccaacacg
    31021 tctccgtcgt aaatggatcg tttgtggcga cgtatctgca cgatgcgacc accagtgtga
    31081 aggtgttcga tctaaacgga aagtttgtcc gtgatgtgca gcttccgggc ctgggaacgt
    31141 cgatgggatt tgccggtcgc agggcgtaca aagagacata ttatgcattc acaagctata
    31201 atatgccggc gacgatttac cgtctggatc tgaccagcta cgaaagcaaa gtctggaagc
    31261 agccgcagct caaattcaat ccgcaggact ttgaaaccaa gcaggtcttt ttcacgagca
    31321 aagacggaac caaagttccg atgtttttga ccgcgaaaaa gggaatcaag caagatggac
    31381 tcaatccaac gtacctgtac ggttacggtg gctttaatat tccgattctg cccagcttca
    31441 gcgtcgctga tctggtgtgg atggaacacg gcggactgat cgcttacgta aacctgcgcg
    31501 gcggtgggga atacggtgaa gagtggcatc gcgccggcac caagctgcgc aaacagaacg
    31561 tgttcgatga cttcatcgct tcggcagagt ggcttatcaa aaacaagtac acgtcaagca
    31621 gcaagctgtc gattgcgggc ggatcgaatg gtggacttct cgtcggtgca tcgatcacgc
    31681 agcgaccgga tctgtttgcc gctgcgattc cttcggtcgg cgtgatggac atgctgcgct
    31741 tccacaagtt cacgatcggt tgggaatgga tcgatgacta cggaagctcg gacaatccgg
    31801 aagaatacaa agcgctgcgc gcctactctc cgtaccacaa catcaagccg ggaacggcgt
    31861 atccagcgac gatggtgacg acggcagatc acgatgatcg cgtcgtccca ggtcacagct
    31921 tcaagttcgc cgcagcactg caagcggcac aaaaaggaaa cgcgccagtg atcattcgca
    31981 ttgatgtgcg cgcaggtcac ggcgccggca agccaacgtc caagcgcatc gacgaagcgg
    32041 cggatcggct cgccttcctg ggcaagaact tgggaatcga ttttagcaag taaaggggac
    32101 tcctcatggc tggcagtgtg aacaaagtca tcgtcatcgg acatctggga gcgaatcccg
    32161 agctgaagta cttgccgtcg ggacagccag tctgcgagat gcgggtagca accagcgaga
    32221 cctaccgcga tcgcaacgat cagccggcag aacggaccga atggcaccgc attgtggtgt
    32281 ggggcaagac ggcggaaaac tgctcgaagt ttttgcaaaa gggccgccag gtctacgtcg
    32341 aaggccgcct gcagacgcga tcgtgggacg acaaagaagg caaaaaacaa tacatgacgg
    32401 aaatcgtcgc aaaccaagtg gtgttcttgg gcggcggtgg tcgtgaagaa ggcgcgcgcg
    32461 aaagcggtgg cggtggttat tcgcgcgaaa gtggcggcta cgcgcgggaa gcagcgtatg
    32521 gcggtggcgg cggtggtggt gggggtggtg gtggcggcgg tcgctacgga cgatcggccc
    32581 ccgcggaagc tgcgccgatg gctgcggaac cgggcgactt tgccgcggaa gacgacattc
    32641 ccttttaggc catcctgatg ttctgttggt acgaactacg gacgaccgac gtgtcggcgg
    32701 cgcagcgctt ttacgccgcg gtgatgcagt gggaaacccg cgatcctccc ggtcccggtc
    32761 tgttttggca ggctgaccgg gcggttgcgg acgtttcgcc gctgtctctg caggcgcggc
    32821 aaaacggcgc tccggcgcac tggctcggac acttgagcac cggcgatctc gatcggacga
    32881 tggggaatct gatcgccgcg ggagcacttt tgcgcggacc tgtgcgaacc ggccaagacg
    32941 gatcgctcgc ggtgctgcgc gatccgcatg gcgcgatggt ggggctgtcc acagaccgca
    33001 cgaaacagag tcaagcggtg tcctggcacg agctccacgt actcgatgcc gaaaaagcgc
    33061 tggcgctgta ttgcgagcag tttggctggc agccgcaaga agtcctcgat ctgcccatgg
    33121 gaatcggtcc ttaccggatg tttgcatcgc aggctggaaa gactccgtgt ggcgcggttg
    33181 cgagcatggc ccatcgcccg caggttcata cccactggct gttttacttt gcggtcgatg
    33241 atctggatgc ggcgtgcgcg cgtgtggtcg cgcagggggg cgaagtgttt ttcggcccgg
    33301 tggaagcgtt tgggagccgt cgaatcgcag tctgccacga cccccaaggc gcagagtttg
    33361 cgctgcaagc gtccgcccca atcccctgct gacaggcggc tccgtccgcg gtagtctgct
    33421 agctatgtcg gttcgtcgaa gcgttgctcc ggatatcgca ggctgtacgt tcgttctgtt
    33481 tgccgtcacg ctaagctttg ccgtgctgcg agcgccattt atccacaagc aggcaggtgc
    33541 gatggcgcaa gacctcctgt cctacggtgg gctgatgttt ggctgcatgc tgaccgccgc
    33601 gggtctgttt cgccgcaagc tgtgggcttt ttttcctgcg gtgctgctct ctgtggtcgc
    33661 aatctacgcg gcagccatca actggcacat gccggaaacc ggacagcttc cggcgggagt
    33721 gctcggactc accacctgcg tcctactttt actgcgctat cgtgaattcg gcggagagac
    33781 ccagccagaa tcctcacgtc agagcgcgga gtaggccccc ctcctcacct tctttgtctg
    33841 tttttcccga cggaagcgca cgaaaaatac gcattttcat cgcttcatat ttgtaggcaa
    33901 agaaacaccg cggaaccgca tcccctggat gaacagatcg tgctacgttc aaagccaagg
    33961 tcttcatgat cgattggtgg ggggacagcg atgtccgaag cagcagacaa gcagaagcgt
    34021 atccgtcgtg gagcactcgc gcccaatcct gagctgtggg ccgcgcttga gcagggtgaa
    34081 aagctcaccg caattttgac cgagttttac accctggcat ttgtcgatcc gcgactgagt
    34141 ccattttttc gcgatgtcac caagcagcgg ctgatcgaaa aacagtacaa cttcatgtac
    34201 gaaatcctga cgggcgagcc ggtctatttt ggtgaac
//
LOCUS       DAORGN010000026        33772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_473_length_33772_cov_6.322063, whole genome shotgun sequence.
ACCESSION   DAORGN010000026 DAORGN010000000
VERSION     DAORGN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 33772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33772
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_33772_cov_6.322063"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2222
                     /locus_tag="PKL17_04935"
     CDS             <1..2222
                     /locus_tag="PKL17_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNK44104.1"
                     /translation="QSVYATDLLGRVTYINAAAEALYGWKKEEVLGKSLLSVAVAPGK
                     EPEANALLDRIHRGESWRGEFPLRRKDGHRFLAEVVGTPALSSSGKVIGLVGISQDIT
                     TRKNAEAALRESEALLRSIADGVPSSILRWDRQLRLTFVNREGVQIAGKPREELIGKH
                     YADVMSEAFFRHNLPRLQAALRGTPSEYEIAPPAPLGKDRSRWYWVKYIPHIIGNEVT
                     GILVLATDITQMKRDAALQTQLSEIVESASDAIVRAGRDGVVQAWNAAAERLYGYSAA
                     EMIGESFDKLIPTELLPAAHDIVARALQGERIGQFETRRQRKDGSLADVELTVSPVRD
                     ACGDIVAVSAIVRDLSARKLLERQLEDARRLETIGRLAGGIAHDFNNILQVMMLHLEH
                     LERQTHLPGSLLSSIKQLEHLADRAAKVTSQLLLFARKQTVQMQTIDANAVLQQTASL
                     LSRILGEHIDLRTKLSPFALWTEADSSMLDQVILNLCINARDAMPQGGTLELSSAMVS
                     WEGAPPERFPEAQPGRYVLLTVSDSGCGMSSDVLSHLFEPFFTTKDAGKGTGLGLAAV
                     YGIVKQHHGFIVVDSTVGQGSTFRIYFPWSDKVQPPHDTSQQTANTEPANPYTLLLVE
                     DEDMLRQIGVAMLTSEGFRVLAVADGRQALRVFDAHHDEIDLLISDMVMPGGISGMDL
                     ATTLRVRNPKLPIILMSGYSEEIITAESNPQTHIEFLAKPFRYPELLLQIRRCLPHS"
     gene            2283..4211
                     /locus_tag="PKL17_04940"
     CDS             2283..4211
                     /locus_tag="PKL17_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HNK44105.1"
                     /translation="MHLVPQPLSTLLARLVHECTTDAKAPIFDLPRHRFYPGSPVDLS
                     VSLHGRRAANPIGPAAGPHTQLAQNLVLSWLGGARVMELKTVQINDTLDIPRPCIDAE
                     NVGYNVEWSQELRLPESLFEYAKGFLLLAALRKLNPQGLRAAELDTLFDISVGYSLDG
                     IRSPQVANWLDTMRSARSLLVQLRDEVPSELRRYLPDELPDELSGCVTLSTFHGCPPD
                     EIERIVLHLFARHQMDVVVKLNPTLLGFDRVSDLLHQRLGYSELQLDPHAFESDLRWS
                     DALAMIARLKTAAQRAGCRLGVKFSNTLVVKNHKQFFPATEKLMYLSGQPLHVLAISL
                     AAEFAQATDGQVPISFSGGIDQKNCSDVVACGLLPVTVCTDLLRPGGYGRMAKYLEPL
                     PAKFAQAGATTIAELVEKNGGMAKCLADYAQKVVADPRYAAAKNQGRPRRVDSKLWMF
                     DCLSCDKCIAVCPNDANFAVNTPQVDPIPVSDVVLRDIGTLVHEPADPFALRENVQIA
                     NFADACNECGNCDIFCPEWGGPYKLKPRFFSSLQSYQVAHMQDGFVVSAGRIQGRISG
                     VEHTLVFGDTSDVFDDGEIAVSVGKTGALESAQIVGKPSVGHRLRLWNYHAMRTLYHA
                     LSQSSNFVSASTLSPVRR"
     gene            4237..5301
                     /locus_tag="PKL17_04945"
     CDS             4237..5301
                     /locus_tag="PKL17_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNK44106.1"
                     /translation="MAGTVATQRFGFIDWFRGLACILMIQTHAYDSWTKEPYRQGTWW
                     IWARLKLGGLPARMFLFLAGVSLAMRFASDARKAVPVAEARRGAFSRGLEVFLIGYGF
                     RIAEWLLGGGKVKDALSMLKVDVLQCIGLSLMITAFVASPRDLDEKRVPWKPLGLALV
                     VAAIVPTLQKLGAPGGPGPLVAYLWPGAHPLGQFPILPWLSYTLTGAAVGALWLRAAK
                     NDKLDAAMGWTVLAGLLVWLCGYGLEKAHLPIFSSTKTVRVEPPFIFFYRTAFCLIGT
                     AASYLIERLRLTDGFSPTRLLGQASLLVYMVHLDIVYNSSPYTIKQKLDPATATMLWI
                     VVAILMVVVAWFRVEGDRKT"
     gene            5346..6380
                     /locus_tag="PKL17_04950"
     CDS             5346..6380
                     /locus_tag="PKL17_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNK44107.1"
                     /translation="MSLAPPQPVPLAELVLAYLDELAHVRRLSTETVRAYRANIVEFL
                     LAAESRRKRPLAVEDLDLWAVRSFLAAQHGKEKPLTTSRKLSALRSFFEYLRRKKLIS
                     ENVAKLLARKKAELPLPQFFTPEQTSALLDPMLPTESDDARKKAVTLRDRAILELIYG
                     GGLRVSEAVNLNLADVSPEDPGQTGDEPILLVRILRGKGRKDRIAPAGRKATEALGAY
                     LPFRQSLAHPKTFAVDEKALFLSVRGQRLGVREVRRILDRATAQSGLPQSHPHALRHS
                     YATHLLGSGADLRSIQELLGHQNLSTTARYAHVDLQYLWDQYQHHPHAQSRANPTENT
                     DRGRKDHKAP"
     gene            6377..6916
                     /gene="hslV"
                     /locus_tag="PKL17_04955"
     CDS             6377..6916
                     /gene="hslV"
                     /locus_tag="PKL17_04955"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNK44108.1"
                     /translation="MTEKIHATTVLCVRKGPQVAMASDGQVTLGNTVVKATAKKVRKM
                     AEGRVLGGFAGSAADGIALFERLEAKLKEHNQQLLRAAVELAKDWRTDRALRRLEAMI
                     IVADRERTLILSGGGDVIEPENGIAAIGSGGPFALAAARALVDATDLSAREISERALR
                     IAGEICIYTNTQLCFEELS"
     gene            6913..8307
                     /gene="hslU"
                     /locus_tag="PKL17_04960"
     CDS             6913..8307
                     /gene="hslU"
                     /locus_tag="PKL17_04960"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNK44109.1"
                     /translation="MSERREAKDLTPRAIVEELDRYIVGQRDAKRAVSIALRNRWRRQ
                     QVSGHLRDEITPKNIILIGPTGVGKTEIARRLAKLVGAPFVKVEASKFTEVGYVGRDV
                     DSMVRDLIEVSIKLVKDEERAAVEGRAKEAAEERLLDVLLPPPTRNAGGVQTRQSPPP
                     VEVSSDDGSHTREKLRELLRQGKLDSREVELEVSEPQSGGGVEAIAGQNIEQMGFDLS
                     RLQDLFGGGRKKKRKVKIPEARELLTSEEAQKLIDMDKVRREAVHRAEQAGIIFIDEI
                     DKIAGRERASGPDVSREGVQRDLLPIVEGSTVTTKYGPVKTDHILFIAAGAFHVSKPS
                     DLIPELQGRFPIRVELQPLTQADLVRILSEPENSLCRQYQALLATEGVKLSWHSDAIT
                     EVARIATDANRMLEDIGARRLQTILERVLDELSFEAPEVGRHGDWAVEVTAEYVRQRV
                     ESVLSNPDLSKYIL"
     gene            8475..8753
                     /locus_tag="PKL17_04965"
     CDS             8475..8753
                     /locus_tag="PKL17_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44110.1"
                     /translation="MMRIRSLCALALCLLPLLGCTETKKEWRKDGIPKDVVKRQQALC
                     EYKAELDAGSPAGLSGDAKDARKSQVRKFTELCMKANGFKEEEITEYL"
     gene            complement(8737..9861)
                     /locus_tag="PKL17_04970"
     CDS             complement(8737..9861)
                     /locus_tag="PKL17_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNK44111.1"
                     /translation="MWQKGRFWLRGLGLLLMLLAMSRRADAAPSAQIDVYTMGPGDDL
                     FSRFGHAAICVTDELSPQGRCYNYGTADFSTPGPLTWGVIRGDGQFWVSVVSLPRMML
                     LYQLEDRTLYRQRLPLSDAQTAAIVTALHRADRPEQTLYNYRHFDDNCTTRIRDLVDE
                     VTGGALRRGNLDKRFAPLRARVYDGFAAAPLLSTLSQLVLGRRVDAPITGWDSMFLPD
                     VLRAELASHLLAAPEVVYQRQRSLPPPSNLPPLLFWSLLALGLVFLSRLGRVGRIATL
                     AVLTLCALVPWSLLVVARLPELRVNEALLVLWPTDFVFLQARYSPLYARLRLAGLVGV
                     IVGKLLGILVQPLWGLWLLASLPLLSVWQATTRPRATDIR"
     gene            9991..11553
                     /locus_tag="PKL17_04975"
     CDS             9991..11553
                     /locus_tag="PKL17_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNK44112.1"
                     /translation="MLFTVLPHSELSSPETEPQDAPPQTPPSPRRRFGLLLLAILLCL
                     SSLLIAISAVVVSRKHRQFVVPGIVISGQPLGTIPLHELPKQVHALGERYLEKRVRLL
                     IGHDKSTASLRELGLRVDEESMLRELSSLGHSGEPLADLQSHLHGKHKGFSFQFAVSL
                     DVQKSHQFLIELKDVVDRAAVDARLDLEKHTIAPERPGYLLRVYDSLVSLEYMARTLS
                     TGQALPEVRLAVAESQPVVRKESLGKLDVKTVLASWETRYSTASIDSDRTYNLKVGAD
                     KLNGYVLQPGQVFSFNAAVGDRTEKEGYRVAPVISGGELIDGLAGGMCQIASTLHAAA
                     FFAGLEIVRSTPHSRPSTYIPMGLDSTVVYPSVDLKLKNPFDFPVVIHYVVHQGVVRV
                     ELLGKERVFKVAFEREILVETPFATVTRPDPEMPTGQQFIEQDGYPGYRIKRRRYIFQ
                     GKWKTDPQDENRGNPEELISRREWDLGYPATTQFVRVGTGSPKLPKKTPPPSHRIPPL
                     PKWAKPLWVIER"
     gene            complement(11611..14499)
                     /locus_tag="PKL17_04980"
     CDS             complement(11611..14499)
                     /locus_tag="PKL17_04980"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNK44113.1"
                     /translation="MKEVSLAKAYDPAEVESKWYKVWEDQGYFHASATTPKTPFTIVI
                     PPPNVTGSLHMGHAMYVVQDVLVRWRRMQGYNALWLPGTDHAGIATQLVVERMLAKEE
                     GKSRHDLGREEFTKRVWQWKDKYGSRIIEQLRVLGFSLDWQRCRFTMDEGLSRAVVEA
                     FVRLYEDGLIYRARRLINWCTRCYTALSDLEVEAEETDGKLWHIAYPVVGSNERLVVA
                     TTRPETLLGDTAVAVHPEDPRFAHLIGKQVQLPLTDRTIPVIADPVLVSMEFGTGAVK
                     VTPGHDFNDFETGLRHNLDQISVFDLGGKLNEHAPERFRGLTIAESRKAVLEALAEAG
                     LLVEEKAHKLNLGRCQRCDTVIEPMLSLQWFVKTEPLAKPAIEAVEQGKTRFVPEHYT
                     DDYFRWMRNIRDWCISRQLWWGHRIPAWYDPQGNVYVARTFEEACQRAGRSDLSQDDD
                     VLDTWFSSALWPFSTLGWPDQTRDLKTFYPTSVMETGHDILFFWVARMMMMGLHFMKK
                     VPFRSVLLHALVVDENGEKMSKVKGNVLDPLHLVYGAKMEEVLGFPKDKAPAKKELDE
                     QLGKFKKAFPSAAAAYPNGFPRQGADALRFFLLVMGAQGRNIRLSVPRIDGYRHFLNK
                     IWSATRFFLMNVDDSEVESLANFREALLHQRGAIESESVAQSAKIELGLSERYILSRL
                     TQVTEEVDAAFSASKFSDGAQALYRFFWTELCDWYIEISKPSLSPSASPEQRRAALGT
                     LTMCLETTLRLLHPIIPFITEELWLRLPRPAGSPQSIMITLYPTSDAVLKDPVAERHM
                     AVLQSAIAAMRSIKSTYNLKTQKSEDGTESQRVQFAVKCPDAELRSVLSANKATIELL
                     TRAELSQVVEDAPTKGTIAEVVPGLTVLLLHAAELIDAPAELARLDKDIQKVEKELQT
                     VQGRLQNADFVARAKPEVVEQSRARVEELTQRTAELRQHKTAIAEMAR"
     gene            14676..16511
                     /locus_tag="PKL17_04985"
     CDS             14676..16511
                     /locus_tag="PKL17_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNK44114.1"
                     /translation="MPSIPYRCQSSVAKAPRRGSRFSWLSLLLGVALSPLPQPNAKAA
                     AQPSPAAQAATAPNPAPQAPESPLLAALRQELDRSMKRLRLKGYEAPYFIGYTLRDIE
                     EAEVTGKLGAVYSKARDRRRVIHVEVRVGNYDFDNTSAGESEGDADLALSLSEVSKDA
                     PLDDNLDALRGSLWLLTDQKYKAALASYARKRARGVRDVEPEDKLPSFSREKPQKFVG
                     DMPRYEVDLDKLSDIVRVAGARLKQAPVLDGEVKVNADRVSRYFVSSEGSETVEHRTI
                     FAAHLSAEARAKDGMLLTNERDFYGRSAEQLPSAQALATAVDGLVRDLSLLQAAPAAD
                     PYNGPAILMEEAAGVFFHETIGHRLEGERQVSEQEGRTFKGQIGQKVLPPFLSVLDDP
                     TLATAPDGTLLNGFYRYDDEGVPARPVTLIEKGVLRDYLKSRTPVLGSLRSNGHGRAE
                     GTSDPIGRMANLLVRAEKTVPLAELKAQLLAEVRRQGKPYGLIIRDILGGSTNTMSFG
                     SQTFRGQPTMVYRVDAKTGEETLVRGVEMVGTPLSSVAKIVAASDTVGVFNGFCGAES
                     GMAPVSTIAPAVLFTEIELQRAQHVSERPPILPPPWRDDAPLPKK"
     gene            16520..17872
                     /locus_tag="PKL17_04990"
     CDS             16520..17872
                     /locus_tag="PKL17_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNK44115.1"
                     /translation="MASTLDPSQAIRRDPDQTCSDLLRFVDASPTPYHAVETAAAQLR
                     AAGFRPTVPSEDFAALTPGPYYTTSSDGTILAFVRPKTRVQGFRIIGAHTDSPNLRIK
                     PRPLYEKVGYLQLGVEVYGGVLLNSWLDRDLRLAGRVMVRGVEGKLTSRLVSLSQPVV
                     RVPQLAIHLDREVSEKGLLLNRQEHLVPILGLADAKKPRQAGDSELLRELCAAQLGLG
                     PNDIVSFELHLHDSTPSCRAGLAQELIFAPRLDNLGMCHAGLQALLRTFEFDDERGIT
                     PVLALFDHEEVGSASDHGAQSATLPRLLERLALSTGCDREKYHQALSRSLCVSADMAH
                     AVHPNYPDRHEANHRPQLNMGPVVKYNSQQRYATSLPTAAQIFELGKKAELSLQEYVH
                     RTDLPCGSTIGPIVSTLLGIPTVDLGNPMLSMHSARELAGAADPENMSRLLAHFLLSR
                     "
     gene            complement(17900..19510)
                     /locus_tag="PKL17_04995"
     CDS             complement(17900..19510)
                     /locus_tag="PKL17_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNK44116.1"
                     /translation="MSAAARGDLLLLLHAHLPYVRHPENSYHLEENWLYEAITATYLP
                     LVQELQKFGTTSSGPKLTLSLSPTLCSMLRDKLLTDRYRSYLDRLLRLGEEELTRAKA
                     DPAQSELVRFYLSRFTELRALWRSLDSDLVAAFGQLQDRGTVELITTCATHAYLPVVQ
                     KPSARRAQLIIAVRHYRECFGRYPRGIWLPECGYVDGLDELLAELDLRYFFLDAHGLT
                     YARPRPPLGLHAPIFTRAGVAAFGRDLMSSKQVWSSTEGYPADGVYREFYRDIGFDRP
                     LEQIAPYIHPDGIRVNTGYKYHRVTGARVDLQDKAPYQPELARAKAREHARDFVEKRQ
                     AQIAWLSQRMDRRPVIVSPYDAELFGHWWFEGPMFLGEVLRLCSEPGSVVQLQTASGY
                     LVDHPVNAVCEPAPSSWGEGGYSAVWLDQSNDWIYRHLHHAESRLHELVTKFASKDPQ
                     ESPQQALIQRALTQAGRELLLAQSSDWAFIMKTGTAVSYAESRVKTHLQRFTKLASDL
                     ESGTIDEAFVADCESRDNLFPSLDPKLF"
     gene            complement(19510..21336)
                     /gene="ribF"
                     /locus_tag="PKL17_05000"
     CDS             complement(19510..21336)
                     /gene="ribF"
                     /locus_tag="PKL17_05000"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNK44117.1"
                     /translation="MPVFASYEEFAASSSSLTPCALVIGNFDGVHRGHKALIAEARQR
                     IGASGRVVVLTFFPHPASVLSPQNAKQVLLSSQRKRELLHLAGADDVIEQRFDAEFAK
                     LSATEFVERIILSGPPPHAVCVGHDFRFGQGRAGDAALLGQLLVRYGTVVTQLGAITV
                     STASGDTLICSSTLIRKALGDGDVDRAALLLGRAPEVEGTVVHGHGRGRTIDVPTANL
                     RVHSDVKLRPGVYAAWAEILAPIDPSSPSVLRPVAARHPAAVNLGYNPTFQGPGTVET
                     SPLSVEAHLLLAAGETLPSLYGGELRLCFVARLRDEKKFSQVAELVAQIHKDIATTRQ
                     LTGGTPSADRDVLAARDGAGVAMGSGTDREAGGRPPHDGGHSSSAAVFTHQDGSGQGD
                     SSPEPKQRRRAAHDDALPETYGRDEVEILSKDPHWYFIYWEVTAAGLAQAQSQLGLSE
                     GDGRLILRVFITTQLPQGRENREIRDLSLHQHHGKKYLESPKAGAHLRAAVGLVSKDG
                     LFSPIAQSQSVRLPPMHPSSETAIEWAHVQPVSGDGKQREHIVLSRPQTPHKERELPW
                     RTGSSPPSGQLPVTDSEWIVDAPSSSPTSSGLTPQPGKSGGL"
     gene            complement(21445..22812)
                     /locus_tag="PKL17_05005"
     CDS             complement(21445..22812)
                     /locus_tag="PKL17_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNK44118.1"
                     /translation="MSDQFTSVEEKGLGTNLVDSIKGVAVGGLLFICSFIVLWMNEGR
                     VDLSEVAKKSVVANPASVDSGLNGKFISVTGDLKTDEKIGDPELLNPGNYLRLHRNVE
                     MFAWIETVKTSTEKKTGGKKVETRTVTYSKGWTDKPKLPNEMEQPKGHENIPLTIKDE
                     SFSAQKAQVAAYPFEVAEASLPSAEPLKLSQDMVKGSSEEAKTDAAPADAAKPADGEK
                     AEGDAVKPAEDKAEDKADEDDQPKKKDKKKKRKGRSVKKPKPLPAEVGKAERKADAIA
                     ARKPVSFSLVDDKYLFKGSGTLAQPEIGDVRISFSALRPGAKVTLFGVLDGGQVRAYK
                     DKESTLFRALAGSREEAIQTMHSEYQTVGWLLRLVGFLMMWFGLILFFGPINTLLDVI
                     PFLGSAGRFLIAVAMFPIALLLSFVTILLSIIAHSPILLTLVFVGMAAGGYFLYQKKK
                     KKAAA"
     gene            23006..23224
                     /locus_tag="PKL17_05010"
     CDS             23006..23224
                     /locus_tag="PKL17_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44119.1"
                     /translation="MLRHTANRKPAFHRRHLSTVSPAASWDCGPQPLQLELSLPPEQR
                     QSAHSTSEKLAGSCVLVINIAGDDEETD"
     gene            complement(23221..23889)
                     /gene="coaE"
                     /locus_tag="PKL17_05015"
     CDS             complement(23221..23889)
                     /gene="coaE"
                     /locus_tag="PKL17_05015"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011014305.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNK44120.1"
                     /translation="MDRPFIIGLTGGIASGKSTVGKILRELGAAVVDADEVARDVVLP
                     GQPAYRKIVEAFGKGILTEPVTESGSEPPIDRKKLAARVFSDESSRRILNQITHPAIA
                     AESARQMQTATAQGAKVIVYEAALLIENNIYQGMDGLLVVDIPTELQVERAVRRGGLS
                     ADEAQARIRSQVSRSARRSAATWIVDNQGGPDDLRRQVLALWQELVQGRLPPAHKPAI
                     ADPC"
     gene            complement(23896..24300)
                     /locus_tag="PKL17_05020"
     CDS             complement(23896..24300)
                     /locus_tag="PKL17_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44121.1"
                     /translation="MKHLRSLFLVSVLAGSVFAVVSSCNTTEMPAESTDMATRTCCGK
                     PGDVGNSLGVGKYCSSAAGAECRTNTSATVCSQLLQDPTRPTYFCTQPCVPDAGTNTC
                     GDAAKCTQDKGTQQWGCVPSVCVDTPAPGCML"
     gene            complement(24494..24907)
                     /gene="ndk"
                     /locus_tag="PKL17_05025"
                     /pseudo
     CDS             complement(24494..24907)
                     /gene="ndk"
                     /locus_tag="PKL17_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            complement(25040..25351)
                     /locus_tag="PKL17_05030"
     CDS             complement(25040..25351)
                     /locus_tag="PKL17_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44122.1"
                     /translation="MAQKHWMVLPAEDALPILRALGVDPKLLKICERRRRIQQAVLAL
                     RESDHLGCVCIGKEHYDVYQIGSQDALCLSLECFGLNQILLLLCYHWLSPISAQPAGD
                     M"
     gene            25640..26248
                     /locus_tag="PKL17_05035"
     CDS             25640..26248
                     /locus_tag="PKL17_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK44123.1"
                     /translation="MSKTSSTKPRSAQPSSAQTKSPRSRSGTQPPVEPKRCLRCGAKA
                     VKPRLGPGRTTRYRTMPTLAIPEDVAIPACGRCQSEYLDEESALAIQPRLQTAYLLAL
                     HVRTRLAIDVLSQHISQRRLEQLLGLSQGYLSRIRAGAGNPSPELVSHLALLAQDPKS
                     RLFELERFWALPDDNWLETAPRPTHKRTRLRPERDVDHAYSD"
     gene            26283..27014
                     /locus_tag="PKL17_05040"
     CDS             26283..27014
                     /locus_tag="PKL17_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK44124.1"
                     /translation="MLLLSALAFSIPSPGGKELDLFQLVLSSGGVVLFVLFLLIVFFV
                     VTTFVIGYKGYQLVLASRETDQFLDTFWQVKRLEKVYDRAKELRRSPVAAQFRAGYLE
                     LVRLTKKRDEAGSEGGGDAESDLENIERALRRAAIAETTSMEALTPLLATVGSSAPFV
                     GLFGTVWGIMDAFRNIGSKGTANLATVAPGIAEALIATAIGLVAAIPAVMAFNYFARR
                     IKVLAGEMDTFHNDLLNIIKRNYLN"
     gene            complement(27072..27581)
                     /locus_tag="PKL17_05045"
     CDS             complement(27072..27581)
                     /locus_tag="PKL17_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44125.1"
                     /translation="MAGLSRQTWGDLALGTGVGVVVLALYFVDGVPGWLRGLSGAVIA
                     LSFVLPLVPYVALIPWYFWAKHRGLVPRSATPYVVAVADGFLIVEHETKGGRYRLDSV
                     VRARLARNSNWGESKLLDDALGLFADSGAEITRLPLDSSGIDALLSSLAAQKIPVHEV
                     DVSAPALID"
     gene            complement(27635..28957)
                     /locus_tag="PKL17_05050"
     CDS             complement(27635..28957)
                     /locus_tag="PKL17_05050"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNK44126.1"
                     /translation="MSKEKSSSTPVPAPAAQTATQHPKGNLGAIVNQHIDRAAKAISL
                     PEEIALILGQPKNEIIVHFPVRMDNGKYQMFTGYRVQHNNILGPYKGGIRYHQDVCLE
                     EVRGLASAMTWKSALHDIPFGGGKGGVQFDPTLHSIGEIERITRRFTHALASSIGPEY
                     DIPAPDVGTNSQVMNWLMDTYMNIAGALDKNAMRRVVTGKSITSGGSLGREAATGQGC
                     VHCITEWAKDRRFDLTGKTVAIQGFGNVGSFTAKILSKLGVSLVATGDYKGYIANPEG
                     LNPHKLAEHVRKTGSVVDYKHSKPISREEFFAVECDILVPAALELEIGEQEARNIKAR
                     LIVEGANGPTWPEADAILNERGITVVPDILANSGGVTVSYYEWLQNKRSERWDLEEVE
                     ARLEKQMKRSYGWVRDFAHEKRLDLRTAAYAIGLDRIRRAYGDRGIWP"
     gene            29148..30545
                     /locus_tag="PKL17_05055"
     CDS             29148..30545
                     /locus_tag="PKL17_05055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44127.1"
                     /translation="MTADSLSGSLLPHKLWMEPSGLAGQAVVRIFDAVQQTARSKGWF
                     AASALVGRSLRDDRKLHSRERRYVGDTVFAMLRKQRRLRALCGGKNPSGQAIYAAFLV
                     DEQTAHSDAELDALSRFLRSAGLSLPDVKVRRQDIEQRFSDIASASPDAQATLIGEAL
                     SYPDWLTSLFVSEFGLAQAIAILHAQNGRARLTLRANTLRCSRDELCAKLHALGIAAE
                     PTPFSPWGVVLDTHLNVYSLPLHQEGFCEVQDEASQLVTELCAPPPGGLVIDACAGAG
                     GKTLGLSAMMGNRGRILAFDIDDKKLSELKQRARRAGLTNIETRCLREDGLLHLVPER
                     ICGAGAQRVLVDAPCSGLGVLRRNPESRWRLTPGDIDEITVKQRQILAASARLVAPHG
                     RLIYATCTILRRENDAIVDDFLASHPDFVPMPVKEILSSSRAAPLGDGERMRIYPTEN
                     GPDGFFAAVLRRKAD"
     gene            complement(30525..31511)
                     /locus_tag="PKL17_05060"
     CDS             complement(30525..31511)
                     /locus_tag="PKL17_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNK44128.1"
                     /translation="MSAREITFLGTASQVPTRHRNHNGLFIRWDTLGILVDPGEGTQR
                     QLLLAGIAATQITHILITHFHGDHCLGLAALSQRISLDKVPHPVEVIYPASGQVFFER
                     LRYASIYKEQAQLVPVPLALPTGDDGNPAVGPLLATEIAAAKVKIWARPLSHRVECFG
                     YRIEEADGVRMLPDKLAELAVRGPDIKKLQQSGTIVVGGKTISIGEVSVPRLGQKLAV
                     VMDSRPCAQAVELARGVDVLVSEATFTREHEEEAHAFAHMTAAQAAQIAVQAQARKLV
                     LTHFSQRYTSTAGHVAEAAAIFPDVVAAEDFARVAVPPRLEESEAGSVGLAP"
     gene            31620..32156
                     /locus_tag="PKL17_05065"
     CDS             31620..32156
                     /locus_tag="PKL17_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44129.1"
                     /translation="MQRRNRHQQTFLALSLLLFACGKNPEQKAAEIGKEVDAALSTQH
                     WEQAEKKAQEILAIAKISDATRDQAKLKLEQAKSEQLAKTLYQKFSGAAGTDHDTAMA
                     AFRDMAQGSYYRGLAKTEYERILPSYIEDHLEKANSASFNGRCADAKQQLQMVFDVDP
                     QNQKAVELGKKPCPKKAD"
     gene            complement(32172..33554)
                     /locus_tag="PKL17_05070"
     CDS             complement(32172..33554)
                     /locus_tag="PKL17_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44130.1"
                     /translation="MRKYVYAASLLSLSSCSGLWTTYQVLEDMPMSDVADMAENPDLL
                     VDPCNGKQYYKMDSAEPTLVACCSNPDQNNGGTNQSNVMATIRDSQNSTMFPGNSVTI
                     RFSCAGTALGTMGFSRADASVSALFAPITRQTVIDGLPLNMSSANSMYPTVLHANQNS
                     RHFIVNSGGSLWLQNLHLKGGKATDMPAAHQSAYPAAQGKAPVAAGGSIVSMGEIKLD
                     RVAFLDNRVEGSAHIRGGAIFILNGKLSTNNSIFYGNNLSALNGATLAATDQAAGAAI
                     SVVNGNVLIGSSTILKNEALSGYNGTEYKGVIDVFEQMTGAGGAYSITVEKSLIVNSL
                     ISSAPAVNTAVNYGLSVNCTTGVQLNGSVSAGNVIDTVNNQLNKSTGKASCPSFTAPS
                     SPIPGELVAKTAIFNHPISRSHPGGQIVASGLTDLLLIGAAKLSCAGDLDFFGVNRVG
                     TLCMPGAEVP"
BASE COUNT         6906 a        10504 c         9794 g         6568 t
ORIGIN      
        1 gacagtcggt atatgcaacc gatctgctcg ggcgggtgac atacataaat gccgcagcgg
       61 aagcgctgta tggatggaaa aaggaagagg tcctggggaa atcgctcctg agcgtggctg
      121 tcgcaccagg aaaagagcca gaggccaatg cactgctcga tcgcatccac cgcggtgagt
      181 cttggcgtgg cgagttcccg ctgcgcagaa aggatgggca tcgttttttg gccgaagttg
      241 tcggaacccc agcactcagc tccagcggca aagtgattgg attggtcggt atttcccaag
      301 acatcacgac acgaaagaac gcagaagcgg cactccgtga aagtgaagcg ctgcttcgct
      361 cgatcgccga cggtgttccg agcagcatcc tgcgctggga caggcagctt cggcttacgt
      421 tcgtcaatcg cgagggtgta cagatcgccg gaaagccacg cgaagaactg attgggaagc
      481 actatgcgga cgtcatgtcg gaagcgtttt tcaggcacaa cctgccacgc ttgcaagccg
      541 cgctccgcgg aacgccatcg gaatacgaaa tcgcgccgcc cgccccgctt ggaaaggaca
      601 gaagccgctg gtactgggta aagtacattc cccacatcat cgggaatgaa gtgacaggaa
      661 tcctggtgct ggcaacggac atcacgcaga tgaagcggga tgccgcactg caaacccagc
      721 tttcggaaat tgtcgaatct gccagcgacg caatcgtccg cgccggacga gacggagtgg
      781 tgcaagcgtg gaacgcggcg gcggaacggc tgtatggcta ttccgcggcg gaaatgattg
      841 gcgaatcttt cgacaagctg attcctaccg agctgctgcc cgcagcacac gacatcgtag
      901 cgcgcgcact gcaaggcgag cgcatcggac agtttgaaac caggcgccag cgcaaagacg
      961 gcagcctggc cgacgtcgag cttacggtgt ctccggtccg tgacgcctgc ggtgacattg
     1021 tcgcagtgtc cgcgattgtc cgggacttgt cggcacggaa gctgctcgaa cggcaactgg
     1081 aggacgcgcg gcggctggaa acgattggca gactggccgg aggaatcgcg cacgacttta
     1141 acaacatctt gcaagtcatg atgctgcacc tggaacattt ggagcggcag acccacttgc
     1201 ctggatcgct tttgtcgtcg ataaagcagc tcgaacacct ggccgatcgc gccgcgaaag
     1261 tgacttcaca actgcttctg tttgcgcgca aacaaacggt gcagatgcag accatcgatg
     1321 caaacgcggt gcttcagcag acagcgagct tgctttcgcg catcttgggt gaacacatcg
     1381 atctgcgtac caagctgtct ccgtttgcgc tgtggacgga agcagacagt tcgatgctgg
     1441 atcaggtgat tttgaacctg tgcataaatg cccgcgatgc gatgccgcaa gggggcacgc
     1501 tggagctttc atctgccatg gtatcttggg aaggtgcgcc ccctgaacga ttcccagaag
     1561 cgcagcccgg acgctatgta ctactgacgg tgagcgactc cggttgtggc atgtctagcg
     1621 atgtcctttc ccatctgttt gaaccgtttt tcacaaccaa agatgccggc aaaggaactg
     1681 gtctgggact ggctgcggtc tacggaatcg tgaagcagca tcacggcttc atcgtagtcg
     1741 acagcaccgt cggacaaggc tctactttcc gcatctattt cccatggtca gacaaagtgc
     1801 agccaccgca cgatacatcc cagcaaacag cgaacacaga gccggcgaat ccatacactc
     1861 tgcttttggt cgaagacgaa gacatgctgc gtcaaattgg cgtcgcgatg ctgaccagcg
     1921 agggatttcg tgtgttggcc gtcgccgatg gccggcaggc gctgcgcgtg ttcgatgcgc
     1981 accacgatga aattgacctg ctgatttcgg acatggtgat gccgggtgga atcagcggaa
     2041 tggatctggc gaccacgctg cgcgtccgca atccgaaact cccgatcatc ctgatgagcg
     2101 gctacagcga agaaatcatc actgcggaat cgaatccgca aacgcacatc gagtttctcg
     2161 ccaagccgtt ccgctatccc gagctgctgc ttcagattcg ccgctgcctg ccacattcct
     2221 aacttggcaa gcgactgact attttgcttg cgctcgtcgc taggttttgg gatttcctcg
     2281 tcatgcatct ggttccgcag ccgctatcta ccctgctcgc ccgcctggtc cacgaatgca
     2341 cgacggatgc caaagcgccg atctttgatc tgccgcgtca tcgcttttat ccaggctcac
     2401 ccgtcgattt gtcggtgtcg ctgcatggac ggcgggcggc caatccgatt ggcccagcgg
     2461 caggaccgca cacccagctt gcgcaaaact tggtgctgtc gtggctcggc ggcgcgcggg
     2521 tgatggaact caagacggtt cagataaacg acacgctgga cattccgcgg ccctgcatcg
     2581 acgcggaaaa cgtcggttac aacgtcgagt ggtcgcaaga gctgcggctg cccgaatcgc
     2641 ttttcgaata cgccaaaggc tttttgctgt tggcagcgct gcgcaagctg aatccgcagg
     2701 gcctgcgcgc ggcagagctt gacacgctgt tcgatatttc ggttggctat tccctcgacg
     2761 gaatccgcag tccgcaggtg gccaattggc tcgatacgat gcgcagtgcg cggtctttgc
     2821 ttgtgcagct tcgcgatgaa gtgccaagcg agctgcgccg ttacctgccc gatgagctgc
     2881 ccgacgagct gtcaggatgc gtcacgctgt cgacgttcca cggctgtccg cccgatgaaa
     2941 tcgagcgcat cgttttgcac ctgtttgcac ggcaccagat ggacgtggtg gtcaagctca
     3001 acccgacgct gctcggcttt gatcgggtgt cggatctttt gcatcagcgg ctcgggtaca
     3061 gcgagcttca gctggatccg cacgcctttg aaagtgatct gcgctggagc gatgcactgg
     3121 cgatgattgc gcggctcaag accgcggcgc agcgtgccgg ctgtcggctc ggcgtgaagt
     3181 tttccaacac gctggtggtg aaaaaccaca aacagttctt tcccgcgacg gaaaagctga
     3241 tgtacctgtc gggccagccg ctgcacgtct tggcgatttc acttgcggca gagtttgcgc
     3301 aagcgacgga cggacaggtg ccgatttcgt tttcgggcgg aatcgatcag aaaaactgct
     3361 ccgatgtggt ggcgtgtggt cttttgcccg tcactgtgtg taccgacctg ttgcgtccgg
     3421 gcggttacgg tcgaatggcc aaatacctgg aacctttgcc ggccaagttt gcgcaagccg
     3481 gtgccacgac gattgccgag ctggtcgaaa aaaacggcgg catggccaag tgtcttgccg
     3541 attacgcaca gaaagtcgtc gccgatccgc gctacgccgc cgccaaaaac caaggtcgcc
     3601 cgcgccgcgt cgacagcaag ctgtggatgt tcgactgcct aagctgcgac aagtgcatcg
     3661 cggtttgtcc aaacgacgca aactttgccg tcaacacgcc ccaggtcgat ccaatcccgg
     3721 tttccgatgt ggtgctacgc gacataggca cgcttgtgca cgaaccggcg gacccatttg
     3781 ccctgcgcga aaacgtccag atcgctaact ttgccgacgc ctgcaatgag tgcggcaact
     3841 gcgacatttt ctgtcccgag tggggcggcc cgtacaagct caagccacgg ttcttttcgt
     3901 cgttgcagag ctatcaagtc gcccacatgc aagatggctt tgtcgtaagc gccggtcgca
     3961 tccaaggccg catcagcggt gtcgagcaca ccttggtttt tggcgacaca agcgatgtct
     4021 ttgacgacgg agaaatcgca gtcagcgtcg gcaaaaccgg cgctctcgag tcggcgcaga
     4081 tcgtcggcaa acccagtgtc ggacaccggc tccggctgtg gaactaccac gccatgcgga
     4141 cgctttacca cgcgctttca caaagctcga acttcgtctc agcaagcacg ctctcgcctg
     4201 tgcgaagata aggtctttcg cgaacgattt tgggcaatgg caggcactgt ggcgacgcag
     4261 cgtttcggtt ttatcgactg gttccgtggc ctggcgtgca ttctgatgat tcagacgcac
     4321 gcctacgact cgtggaccaa agagccctat cggcaaggaa cgtggtggat ttgggcccgg
     4381 cttaagctgg gcgggctgcc ggcgcgaatg tttctgtttc tggccggtgt gtcgctggcg
     4441 atgcgttttg ccagcgatgc gcgcaaagca gtgccagttg ccgaggcacg gcgcggtgcc
     4501 ttttcacgcg gcctggaagt gtttttgata ggttacggtt ttcgcattgc cgagtggctg
     4561 ctcggcggag gcaaggtcaa agacgcgctg tcgatgctga aggtcgatgt gcttcagtgc
     4621 atcgggctgt cgttgatgat caccgcgttt gtggcgtcgc cgcgcgatct ggatgaaaag
     4681 cgagtgccgt ggaagccgct ggggcttgcg cttgtcgtcg cggcgatcgt gccaacactg
     4741 caaaaactgg gtgcaccagg cgggcccgga ccgcttgtcg cgtatctgtg gccgggagcg
     4801 catccgctcg ggcagtttcc gattttgccg tggctgtcgt atacgctgac cggagccgcc
     4861 gtcggtgcgc tgtggctgcg cgcggccaaa aacgacaagc tggatgcagc gatgggctgg
     4921 acggtcttgg ccgggctttt ggtctggctg tgcggctatg gcctggaaaa agcccacttg
     4981 ccgatttttt cgtcgacgaa aacggtgcgc gtcgaaccac cgttcatttt tttctatcgc
     5041 accgcgtttt gtttgattgg caccgcagcg tcgtatctga tcgaaaggct gcgtctcacc
     5101 gatggttttt caccgacgag actgctcggg caagcgtcgc ttctcgtgta tatggtccac
     5161 ctcgatatcg tctacaacag ctcgccgtac accatcaaac agaagctaga cccagcgaca
     5221 gcgacgatgc tgtggattgt ggtcgcgatt ttgatggtcg tcgtcgcctg gtttcgcgtc
     5281 gaaggcgacc gcaagacctg accttgtgca gttgacggct cgacgacagc ttgtatgctg
     5341 ccgcggtgtc gctcgctccc ccacagcctg tgccgctcgc cgagcttgtt ttggcctatc
     5401 tcgacgagct agcgcatgtg cgtcggctgt ccaccgaaac ggtgcgcgcg tatcgcgcca
     5461 acatcgtcga atttttgctg gccgcggagt cgcgtcgcaa acggccactg gctgtcgagg
     5521 atctcgatct gtgggcggtg cgcagctttc tcgccgcgca gcatggcaaa gaaaagccgc
     5581 tgacgacgag ccgcaagctg tcggcactgc ggtctttttt tgaatatctg cgccgaaaaa
     5641 agctgatttc agaaaacgtc gccaagcttc ttgcgcgcaa aaaagccgag ctgccactgc
     5701 cgcagttttt cacgccggaa cagacctcgg cgctgcttga tccgatgttg ccgacggaaa
     5761 gcgacgatgc gcgcaaaaaa gcagtcacac tgcgcgatcg cgccatcctg gaactgatct
     5821 acggcggcgg tctgcgcgtc agcgaggcgg tgaatctgaa cttggccgac gtatcgccag
     5881 aagatccggg ccagaccggc gacgaaccga tcctgcttgt gcggattttg cgcggcaaag
     5941 gccgcaaaga ccgcatcgcg ccagcaggac gaaaggcgac cgaagcactc ggagcgtacc
     6001 tgccttttcg gcaaagcttg gcccatccaa agacttttgc agtcgacgaa aaggcgctgt
     6061 ttctgtcggt gcgcgggcag cggctcggag tgcgcgaagt ccggcggatt ctcgatcgag
     6121 cgacggcgca gagcggtctg ccgcagagcc acccgcacgc cctgcggcac agctacgcga
     6181 cgcatctttt gggatcgggg gccgatctgc gatcgattca ggagctgctc gggcaccaaa
     6241 acctatcgac caccgcgcgc tatgcgcacg tcgacttgca gtatctttgg gaccaatatc
     6301 agcaccatcc ccacgcgcaa agtcgggcaa atccgacgga aaacacagac cgcgggcgga
     6361 aggaccacaa agcgccatga ctgaaaaaat ccatgccacc accgtgctgt gcgtgcgcaa
     6421 aggcccccaa gtggcaatgg cttccgacgg acaagtcacg ctgggtaaca ccgtcgtcaa
     6481 agccaccgca aaaaaagtcc gcaagatggc cgaagggcgc gtcttgggag gttttgctgg
     6541 cagtgctgcc gacggaatcg ccctgtttga acggctcgaa gccaagctca aagaacacaa
     6601 ccaacaactg ctgcgcgccg ccgtcgagct ggccaaagac tggcgcaccg atcgcgccct
     6661 gcgtcgattg gaagccatga tcatcgtcgc ggatcgcgag cgcaccctga ttttgtcggg
     6721 cggtggcgat gtcatcgaac cggaaaatgg cattgcggcg attggttcag gcgggccatt
     6781 tgcgctggct gcggcacgcg cgctcgtcga tgcaaccgat ctgtcggccc gcgaaatcag
     6841 cgaacgggcc ctgcggattg ccggcgagat ttgcatctac accaacaccc agctttgttt
     6901 cgaggagctg tcatgagcga acgtcgcgag gccaaagacc tcactccgcg cgccatagtc
     6961 gaagaactcg atcgctacat cgtcggtcag cgtgatgcca agcgggccgt ttccatcgcg
     7021 ctgcgcaatc gctggcgacg acagcaagta tcaggacacc tgcgcgacga gatcaccccg
     7081 aaaaacatca tcttgattgg cccaacgggc gtcggcaaaa ccgaaattgc ccgtcggctc
     7141 gccaagctgg tcggcgcgcc atttgtcaaa gtcgaagcca gcaagttcac cgaagtgggt
     7201 tacgtcggtc gcgatgtcga ttcgatggtt cgcgatctca tcgaagtgtc catcaagctg
     7261 gtcaaagacg aagaacgcgc cgcggtggag ggccgagcca aagaggccgc agaggagcgc
     7321 ctcttggatg tgctgttgcc gccgccgacc cgcaatgccg gtggcgtgca gacccgtcag
     7381 agcccaccgc ccgtcgaggt cagcagcgac gatggcagcc acacccgcga aaaactgcgc
     7441 gaactgttgc gccagggcaa gctcgacagc cgcgaagtcg aactggaagt cagcgaaccg
     7501 cagagcggcg gcggggtcga agccatcgct ggacaaaaca tcgaacagat gggcttcgat
     7561 ctgtctcggc tgcaagacct gttcggcggt ggtcgcaaaa agaagcgcaa agtcaaaatc
     7621 cccgaagcgc gcgagctgct gaccagcgaa gaagcgcaaa agcttatcga catggacaaa
     7681 gtgcgacggg aagccgtcca ccgcgccgag caagccggca tcatcttcat cgacgaaatc
     7741 gacaagattg ccggacgaga gcgggcttct gggccggatg tgtcgcgcga aggcgtccag
     7801 cgcgacttgc tgccgattgt cgaagggtcg acggtgacaa ccaagtatgg gccggtcaag
     7861 accgaccaca tcttgtttat tgccgccgga gcgttccacg tcagcaagcc gtcggacctg
     7921 attccggaac tgcaaggtcg ctttccgatt cgcgtcgagc ttcagccgct cacgcaggcc
     7981 gatttggtac ggattttgag cgagccggaa aactcgctgt gtcgtcagta tcaagcgctt
     8041 ttggccaccg aaggggtcaa gctaagctgg cacagcgacg cgatcaccga agttgcccgg
     8101 attgccaccg acgccaaccg catgctcgaa gacattgggg cacggcgact tcaaacgatt
     8161 ttggaacggg tactcgacga gctttcgttt gaagcccccg aagtcggtcg tcacggcgac
     8221 tgggcggtcg aagtcaccgc cgaatacgtc cgccagcgcg tcgagagcgt cctgagcaac
     8281 cccgacctgt caaaatacat cctgtagtct tagctacact tcacgaaccg aacccgccca
     8341 agtagccacg gtccgcccag aggacgatgc cgtgccgtcc accactgggc tatgtgagca
     8401 ctttttaatc agcaattaaa acgtgctgta agttactcaa tttttacgct attctctgct
     8461 ccaacgggag attgatgatg aggattcgtt cgctctgcgc cctggccctg tgccttctgc
     8521 ccctcctcgg ctgcactgag accaaaaaag agtggcgcaa agatggcatt cccaaggacg
     8581 tcgtaaagcg tcaacaggcg ctctgcgagt acaaagcgga gctggatgct ggctcgccgg
     8641 cgggactgtc gggcgatgcg aaagacgccc gcaagagcca agtgcgcaag ttcaccgagc
     8701 tgtgcatgaa agcgaacggc ttcaaagaag aagaaatcac cgaatatctg tagcccgtgg
     8761 gcgcgtcgtc gcttgccaca ccgacaaaag cggtagcgac gccaaaagcc aaagtcccca
     8821 caaaggctgc accaagatcc caagcagctt cccgacgatc accccgacaa gcccagccag
     8881 ccgaagccgc gcatacagcg gactgtaacg cgcctgcaaa aacacaaaat ccgtcggcca
     8941 cagcacaagc agcgcttcgt tcacccgcag ctcgggaagt cgcgccacca caagcaacga
     9001 ccacggcaca agcgcgcaca gcgtcagcac agccagcgtt gcgatccgtc cgacgcgacc
     9061 aagccgtgac aaaaaaacga gcccaagcgc aagcagcgac cagaacagca gcggcggcag
     9121 gttcgacggt ggcggcagcg agcgctgacg ctgatacacc acttcgggcg cagccagaag
     9181 gtggctggcc agctcggcac gcagcacatc gggcaaaaac atgctgtccc agccggtgat
     9241 cggcgcatcg acgcggcgac ccagcacaag ctgcgacagc gtcgacaaaa gcggcgccgc
     9301 ggcaaacccg tcgtagaccc gagcgcgcag cggcgcaaag cgtttgtcca ggttgccgcg
     9361 ccgcagcgcc ccacccgtca cttcatcgac caagtcgcgg atgcgcgtcg tgcagttgtc
     9421 gtcgaaatgg cggtagttat atagcgtctg ctcaggccga tcggcccggt gcagcgccgt
     9481 cacaatggcc gcggtctggg catcggaaag cggcagtcgc tgccggtaca gcgtgcggtc
     9541 ttccagctga taaagcagca tcatccgcgg cagactgacc accgaaaccc agaactgccc
     9601 gtcgccgcga atcacgcccc aggtcaacgg ccccggcgtc gaaaaatccg ccgtcccgta
     9661 gttatagcag cgaccctgcg gactcagttc gtcggtgaca cagatggcag catggccaaa
     9721 acgcgaaaac aggtcatcgc cagggcccat cgtatagacg tcgatctgcg ccgacggtgc
     9781 tgcgtcggct cgacgggaca tcgcgagcag catcaaaagc agcccaagtc cgcgcaacca
     9841 aaaacgcccc ttttgccaca tgttgtcgat ctttgcagtc cgcagcggca gccggcaagt
     9901 cggcgttgtt cgcgcaggcg gacttttgct ttcgggctgc ggtctgcggt ttcccttttg
     9961 catcaccttg cgctatgaag cgtgcgctcg atgctgttca cggttttgcc tcatagcgag
    10021 ctctcgtcgc cggaaacgga accccaagac gcgccgcccc aaacaccccc atcgccaagg
    10081 aggcggtttg ggcttttgct gctggcgatt ttgctctgtc tgtcgtcgct gctgattgca
    10141 atttccgcag tcgtggtgtc gcgcaaacac cggcagttcg tggtgcccgg catcgtgatt
    10201 tcggggcagc cgctggggac gattccgctt cacgagctgc ccaaacaagt gcacgcgctg
    10261 ggtgaacgct atctcgaaaa gcgggtgcgg cttctgatag gtcacgacaa gtcgaccgcg
    10321 agcctgcgcg agctgggcct gcgcgtcgat gaagagtcga tgctgcgcga gctttcatcg
    10381 ctcggtcaca gcggtgagcc gctcgccgac ttgcaaagcc atctgcacgg caagcacaaa
    10441 ggcttttcgt ttcagtttgc ggtcagcctc gacgtgcaaa agtctcacca gtttttgatc
    10501 gaactaaaag atgtggtcga tcgagctgcc gtcgacgcgc gcctggatct ggaaaagcac
    10561 acgattgcgc cagagcgtcc gggctatctt ttgcgggtct atgactcgct ggtcagcctg
    10621 gaatacatgg cgcggacgct gtcgacggga caagcgcttc cggaagtccg gctggccgtc
    10681 gcggaatcgc agcccgtggt gcgcaaagaa agcctgggca agctcgatgt aaagacggtg
    10741 ctggcaagct gggaaacccg ctattcgacc gcatccatcg acagtgatcg cacctacaac
    10801 ctgaaagtcg gcgccgacaa gctcaacggc tacgtgctgc aacccggtca ggtcttttcg
    10861 tttaacgctg ccgttggaga tcgcacggaa aaagaaggct atcgggtcgc tccggtgatc
    10921 tcgggcggcg agcttatcga cggactggcg ggcggcatgt gtcagattgc gtcgacgctg
    10981 cacgcggcgg cgttctttgc ggggctcgaa attgtccgct cgacgccgca ctcccgaccg
    11041 agcacgtaca ttcccatggg gctcgattcc accgtggtct atccgtcggt ggatctgaag
    11101 ctgaaaaacc cgttcgattt cccggtggtg attcactacg tcgtgcacca gggcgtggtt
    11161 cgcgtcgagc tactcggcaa agaacgtgtc ttcaaggtcg ccttcgagcg cgagatcctc
    11221 gtcgagactc cattcgccac agtcacccgg cccgaccccg aaatgcccac ggggcagcag
    11281 ttcatcgaac aagacgggta tccggggtat cggatcaagc ggcgacggta catcttccag
    11341 ggcaagtgga agaccgatcc ccaagacgaa aaccgcggca accccgagga gctgatcagc
    11401 cggcgggagt gggatctggg ctatccagcg acgacccagt ttgtccgcgt cggaaccgga
    11461 tcgcccaagc tgccgaaaaa gacgccaccg ccgtcgcatc gcatccctcc gctgccgaag
    11521 tgggccaagc cgctgtgggt gattgaacgg tagaacgcag tcactccggt cggcgcgttg
    11581 tcacaccgcc ggaggctggc atcgaaaaag ctagcgggcc atttcggcaa tcgcggtctt
    11641 gtgctggcgc agctcggcag tgcgctgggt cagttcttcg acgcgagcgc ggctctgttc
    11701 gacgacttcc ggcttggcgc gggcgacaaa gtcggcattt tgaagccgtc cctgcaccgt
    11761 ctgaagctct ttttcgacct tttggatgtc cttatcgagt cgagccagct ctgccggcgc
    11821 gtcgataagc tccgcggcgt ggagcagcag caccgtaagc cccggcacca cctcggcgat
    11881 cgtgcccttg gtcggtgcat cctcgacgac ttgcgacagc tctgcacggg tcagaagctc
    11941 gatggtcgcc ttgttggccg acagcacgct gcgcagctct gcatcgggac acttgacggc
    12001 aaactgcacg cgctggctct cggtcccgtc ttccgacttc tgcgtcttca ggttgtaagt
    12061 cgacttgatg ctgcgcatcg ccgcgatcgc gctttgcaac accgccatgt gccgttccgc
    12121 caccgggtct ttgagcaccg catcgctcgt cggatagagc gtgatcatga tgctctgtgg
    12181 actgcccgca ggacgcggca gacgcagcca cagctcttcg gtgataaacg gaatgatcgg
    12241 gtgcaaaagc cgcagcgtcg tttccagaca catcgtcagg gttccaagcg cggcccgtcg
    12301 ctgttccgga cttgccgatg gcgacagact cggcttgctg atctcgatgt accagtcgca
    12361 aagctcggtc caaaagaacc gatagagcgc ctgcgcaccg tcggaaaact tgctggctga
    12421 aaacgctgcg tcgacttctt cggtgacttg ggtaagccgc gacaggatgt agcgttcgct
    12481 gagtcccagc tcgatttttg ccgactgagc cacgctttcc gattcgatcg ccccgcgctg
    12541 atgcaaaagc gcctcgcgga agttggccag cgactcgact tcgctgtcgt cgacgttcat
    12601 caggaaaaag cgcgtcgcac tccagatttt gttcaggaag tggcgatagc cgtcgatgcg
    12661 cggcactgac aagcggatgt tgcgaccctg cgctcccatc accaaaagga agaagcgcag
    12721 cgcgtcggcc ccctgccgcg ggaaaccgtt tggatacgcc gcagccgccg acgggaaggc
    12781 ttttttgaac ttgcccagct gctcgtcgag ttcttttttc gccggcgctt tgtctttggg
    12841 gaagcccagc acttcttcca ttttcgcgcc gtagaccaga tgcaaaggat cgaggacgtt
    12901 gcccttgacc ttggacatct tttcgccgtt ttcgtcgacg acgagcgcgt gaagcagcac
    12961 gctacggaac ggcacttttt tcataaagtg caggcccatc atcatcatgc gagcgaccca
    13021 gaagaacagg atgtcgtgtc ctgtctccat caccgacgtc gggtaaaacg tcttgaggtc
    13081 gcgcgtctga tcgggccagc ccagcgtcga aaatggccac agcgccgacg aaaaccacgt
    13141 atccagcacg tcgtcgtctt gcgaaagatc gctgcgaccg gcgcgctgac aggcttcctc
    13201 gaatgtccgg gcgacgtaga cattgccttg cgggtcgtac cacgccggaa tccgatgtcc
    13261 ccaccaaagc tgccgcgaaa tacaccagtc gcggatgttg cgcatccagc ggaagtaatc
    13321 gtcggtgtag tgctcgggga caaaccgagt cttgccttgc tcgactgctt caatcgccgg
    13381 cttggcgagc ggttcggtct tcacaaacca ctggagcgac agcatcggtt cgatcacggt
    13441 gtcgcagcgc tgacagcgtc ccaggttcag cttgtgcgct ttttcttcga cgagaagtcc
    13501 agcctcagcc agcgcttcca gcacggcttt gcgcgattcc gcgatggtca ggccgcggaa
    13561 acgctcgggc gcatgttcgt tgagcttgcc gcccaaatcg aacaccgaaa tctgatcgag
    13621 gttgtgccgc agtcccgtct cgaagtcatt gaagtcatga ccaggcgtga ccttgaccgc
    13681 gccggtgcca aactccatcg aaacgagcac cggatcggcg atcaccggga tcgtgcgatc
    13741 cgtcagcgga agctgcactt gcttgccgat gaggtgggca aagcgcggat cttcgggatg
    13801 aactgccact gccgtgtcac ccaaaagcgt ttccggtcgc gtcgtcgcca ccaccagccg
    13861 ttcattcgaa ccgacgacgg gataggcaat gtgccacagc ttgccgtccg tctcttcggc
    13921 ttcgacttcc agatccgaca gcgcggtgta acagcgcgta caccagttga taagccgtcg
    13981 cgcccggtaa atcaggccat cttcgtacag acgcacaaat gcctcgacga cggcgcgcga
    14041 cagaccttcg tccatcgtga agcggcagcg ctgccagtcg agcgaaaagc cgagtacgcg
    14101 aagctgctcg atgatgcggc tgccgtactt gtccttccac tgccaaaccc gcttggtaaa
    14161 ttcctcccgc ccgaggtcgt gccggctctt gccttcttct ttggcaagca tccgctcgac
    14221 gacaagctgg gtcgcaatgc ccgcgtgatc ggtgcccggc agccacagcg cgttatagcc
    14281 ttgcatgcgg cgccagcgca caagcacgtc ttgcacgacg tacatcgcgt ggcccatgtg
    14341 caaagagccg gtgacgttgg gcggcggaat aacgatggta aaaggggtct ttggcgtcgt
    14401 cgccgaggcg tggaaatagc cttgatcttc ccacaccttg taccacttcg attcaacctc
    14461 agccgggtcg taggccttgg ccagcgaaac ttccttcatc acgagagcat ccttcgagga
    14521 gaactggcag tcattgagcc gggccttctt atcacacggg atcatcgtct gcggcaactt
    14581 ttcaagaggc ccgaccaagc cgacggtgac aaaggtccga ctcgtcgatg cattgctgcg
    14641 ctatcatgga tggggtctta actgccgaga actcgatgcc aagcatccct tatcgttgcc
    14701 aatcatccgt cgccaaagcg ccgcgccgtg gttcccgttt ttcctggctg agcctgcttt
    14761 tgggcgttgc gctgtccccg ctgccacagc ccaacgcgaa ggccgccgca cagccaagcc
    14821 ccgcagcaca ggcggcgacc gcgccaaacc cagcgcccca agcgcctgaa agtccgctcc
    14881 tcgcggcgct gcgacaagag ctggatcgct cgatgaagcg gctgcggctc aaaggctatg
    14941 aagcgccgta tttcatcggc tacaccctgc gcgacatcga ggaagccgaa gtcaccggca
    15001 agctcggtgc cgtctacagc aaagcgcgcg atcgcagacg ggttatccac gtcgaagttc
    15061 gcgtcggcaa ctatgacttc gacaacacgt ccgccgggga atcggaaggc gatgccgatc
    15121 tggcgctgag cctttcggaa gtgtcgaaag acgccccgct cgacgacaat ttggatgcgc
    15181 tgcgcggatc gttgtggctt ttgaccgacc aaaaatacaa agccgcactg gcgtcgtatg
    15241 cccgaaagcg cgcccgtggc gtccgcgatg tcgaacccga agacaaactg ccgagttttt
    15301 cgcgtgaaaa accgcaaaag ttcgtcggtg acatgccgcg ctacgaagtc gatctcgaca
    15361 agctgtcgga catcgtgcgg gttgccggcg cgcggctcaa acaggcgcca gttctcgacg
    15421 gagaagtcaa ggtcaacgcc gaccgcgtga gccggtattt cgtcagcagt gaaggaagcg
    15481 aaaccgtcga acaccgcacc attttcgccg cacacctgtc ggcggaagcg cgcgccaaag
    15541 acggcatgct gctgaccaat gaacgggatt tttatggtcg atccgccgag cagcttccgt
    15601 cggcgcaagc gctggccacg gcggtcgacg ggctggtccg cgatctgtcg cttttgcaag
    15661 ccgccccggc agccgatccc tacaacggtc cggcgatcct gatggaagaa gccgcgggcg
    15721 tgtttttcca cgagacgatc ggccacaggc tcgaaggcga acgccaggtc agcgaacaag
    15781 aaggccgcac gttcaaaggg caaatcgggc aaaaagtgct gccgccgttt ctgtcggtgc
    15841 tcgacgatcc gacgctggca acggcgcctg acgggacgct gctcaacggt ttttatcgct
    15901 acgacgatga aggcgtgccg gcccgtcccg tgacgctgat cgaaaaaggc gtgctgcgcg
    15961 actacctgaa gtcgcggacg ccggtgctcg gatcgctgcg atcgaatggc cacggtcgcg
    16021 ccgagggcac aagcgacccg atcggacgga tggccaacct gcttgtacgc gccgaaaaga
    16081 ccgtgccgct cgccgaatta aaagcgcagc ttttggccga agtccgtcgc caaggcaagc
    16141 cttacggcct gatcatccgc gacatcctgg gcggatcaac caacaccatg agctttggca
    16201 gccagacctt ccgtggtcag ccaacgatgg tctatcgcgt cgatgccaag accggcgaag
    16261 aaaccctggt gcgcggcgtc gagatggtcg gaaccccgct cagctccgtc gccaaaatcg
    16321 tcgccgcatc cgataccgtt ggcgtgttta atggcttctg tggcgccgaa agcggcatgg
    16381 ctccggtttc gaccattgcg cccgccgtgc tgtttactga aattgaactc cagcgcgccc
    16441 aacatgtgtc agagcgacca ccgattctgc cgccaccgtg gcgcgacgac gccccgcttc
    16501 ccaagaaatg agcatgacca tggcttccac tctggatcca tcgcaggcga ttcgccgcga
    16561 tcctgaccaa acttgtagcg atctgctccg ctttgtcgat gcgtctccga cgccctatca
    16621 cgccgtagaa accgccgccg cgcagcttcg cgcagctggg tttcgtccca ccgttccaag
    16681 cgaagacttt gcggcgctca cccccggtcc gtactacacc acatcgagcg acggcacgat
    16741 tttggcgttt gtccggccca aaacccgcgt gcagggcttc cgcatcatcg gcgcccacac
    16801 cgatagcccg aacctgcgga tcaaaccgcg tcccctgtac gaaaaagtcg gctacctcca
    16861 gcttggcgtc gaagtgtatg gcggcgtgct gctcaactcg tggctcgatc gcgacctgcg
    16921 gctggctggc cgggtgatgg tgcgcggcgt cgagggcaag ctcacgtcgc ggcttgtctc
    16981 gctttcgcag cctgttgtgc gggtgcccca gctcgcgatt cacctcgacc gcgaggtcag
    17041 tgaaaagggt ctgctgctca accgccagga acatctggtg ccgatcctgg ggcttgccga
    17101 cgccaaaaag ccgcgccagg ctggggacag cgaacttctg cgcgagctgt gcgccgccca
    17161 attgggactt ggtccaaatg acatcgtcag cttcgagctg catctgcacg acagtacgcc
    17221 gtcgtgccgg gccggtcttg cacaggaact gatctttgcg ccgcggctcg acaacctggg
    17281 catgtgtcac gctggccttc aggctttgct gcggacgttc gagttcgacg acgagcgcgg
    17341 catcacccca gttttggcgc tgttcgatca cgaagaagtc ggctcggcca gcgaccacgg
    17401 tgcccagtcg gccacgctgc ccaggctgct tgaacggctg gcgctttcga cgggctgtga
    17461 ccgcgagaag taccatcaag cgctcagtcg atcgctctgc gtgtcggcgg acatggcgca
    17521 tgcggtgcat ccaaactatc ccgaccgcca cgaagccaac cacaggccgc agctcaacat
    17581 ggggccggtg gtgaaataca actcgcagca gcgctacgca acgagtctac cgacggcagc
    17641 gcagattttt gaactgggca agaaggccga actgtcgctg caagagtacg tccaccgcac
    17701 cgatttgccg tgtggcagca cgattggtcc gattgtgtcg acgctgctgg gaattccgac
    17761 ggtcgatttg ggcaatccga tgctcagcat gcactcggcg cgcgagctgg ccggcgcggc
    17821 tgacccggaa aacatgtcgc gcctgctcgc ccactttttg ctttcgcgct agcgtggcaa
    17881 cacgtcgcgt ctgctcgaat caaaacagct tcggatcgag gctcgggaac aggttgtcgc
    17941 gcgactcgca atcggcgaca aacgcttcgt cgatcgtccc cgattcgaga tcgctcgcca
    18001 gcttggtaaa gcgctgcaga tgcgtcttga cccgcgattc ggcatacgac accgccgtcc
    18061 cggtcttcat gatgaacgcc caatccgacg actgcgcgag cagcagctca cgtccggctt
    18121 gggtgagcgc ccgctgaatc agcgcctgct gcggactttc ttgcggatct ttgctcgcaa
    18181 acttggtcac aagctcgtgc agtctcgatt cggcgtggtg caggtgacgg taaatccagt
    18241 cgttgctctg atcgagccac accgcggaat agccgccttc accccacgac gacggcgccg
    18301 gttcgcagac cgcgttgacg ggatgatcga caagataacc gctcgcggtc tgaagctgca
    18361 cgacactgcc gggctcgctg cacagccgca gcacttcgcc cagaaacatc ggcccctcga
    18421 accaccagtg accaaacagc tcggcgtcat acggcgaaac aatcaccgga cggcgatcca
    18481 tccgctgcga cagccaggca atctgcgctt ggcgcttttc gacgaagtcc cgcgcatgct
    18541 cgcgcgcttt tgcccgcgcc agttccggct gatacggcgc tttgtcttgc agatcgacac
    18601 gcgcccccgt cacccggtgg tacttgtagc cggtgttgac gcggatgccg tcgggatgaa
    18661 tgtacggagc gatctgctca agcggcctgt caaagccgat gtcgcgataa aattcgcgat
    18721 acacaccgtc ggcgggatag ccttccgtcg acgaccacac ctgctttgat gacatcaggt
    18781 cgcgaccaaa cgccgccacg ccagcgcggg tgaaaatcgg cgcatgcagc ccgagcggcg
    18841 gacgcggtcg cgcatacgtc agtccgtgcg catccaggaa gaagtagcgc aggtctagct
    18901 cggcgagcag ctcgtccagc ccgtcgacgt agccacactc aggcagccag atgccgcgcg
    18961 gatagcgacc aaaacactcg cggtagtgac ggaccgcgat aatcagctgg gcccgccgcg
    19021 ccgacggctt ttgcaccacc ggcagatagg catgcgtcgc acaggtcgta atcagttcga
    19081 ctgtgccgcg atcttgaagc tgaccaaacg ccgcgaccaa gtcactgtcc agcgaacgcc
    19141 acagcgcccg cagctcggta aaccgcgaca ggtaaaagcg caccagctcc gattgcgcgg
    19201 gatctgcttt ggcgcgggtc agttcctctt ccccgagccg cagcagtcta tcgagatagc
    19261 tgcgatagcg gtcggtcaag agcttgtcgc gcagcatcga acacagcgtc ggcgaaagcg
    19321 acagcgtcag cttgggccca gatgatgtcg tgccaaactt ttgcagctct tgcacaagcg
    19381 gcagatacgt cgcggtgatc gcttcgtaga gccagttttc ttccaggtga taggaatttt
    19441 cggggtggcg cacatacggc aggtgcgcgt gaagcaaaag aagcagatca ccccgagccg
    19501 ccgccgacat cacagacctc cgctcttgcc tggctgtggt gtaagaccac tgcttgtcgg
    19561 actgctcgac ggagcatcga cgatccattc cgagtccgtc accggtagct gcccagacgg
    19621 cggagaactc cccgtccgcc agggaagctc acgctctttg tgcggcgtct ggggccgtga
    19681 cagcacgata tgttcacgct gctttccgtc gccagaaacc ggctgcacat gcgcccattc
    19741 gatcgccgtt tccgacgatg gatgcatcgg cggcaagcgc actgactgcg actgtgcgat
    19801 cggcgaaaac agaccgtctt tcgacacaag acccaccgcc gcccgcaggt gcgccccggc
    19861 tttcggtgac tccagatatt ttttgccgtg gtgctggtgc agcgaaagat cgcgaatttc
    19921 gcggttttcg cgcccctgcg ggagctgcgt cgtgatgaac acccgcaaaa tcagacggcc
    19981 atcgccttca gaaagcccga gctgcgactg cgcttgcgca agccccgccg ccgtcacttc
    20041 ccagtagatg aagtaccagt gcggatcttt ggacaggatc tcgacttcgt cgcgcccata
    20101 cgtttcgggc agcgcgtcat cgtgagccgc tcgtcgccgc tgttttggtt caggcgagct
    20161 gtcgccctga ccactgccat cctgatgtgt aaaaaccgcc gcggacgacg agtgcccgcc
    20221 gtcgtgtggt ggtcgtccgc ctgcttccct gtctgtcccg gaacccatgg caactccagc
    20281 tccgtcgcgc gccgccaaaa cgtcgcggtc tgcagacgga gtccccccgg ttagctgtct
    20341 tgtggtggcg atgtctttgt ggatctgcgc gaccagctcc gccacctggg aaaacttctt
    20401 ctcgtcacgc agccgggcca caaagcacaa tcgcagctcg ccgccataca gcgagggaag
    20461 cgtttcaccc gccgcgagca aaaggtgtgc ttcgacggag agtggcgacg tttcgaccgt
    20521 acccggaccc tggaacgttg gattatatcc aaggtttacc gccgcagggt gtctagccgc
    20581 cacaggccgc aacaccgacg gggacgacgg gtcaatcggc gccaaaattt ccgcccacgc
    20641 cgcatagaca ccgggccgca gcttcacatc cgaatgcacg cgcaggttgg ctgtcgggac
    20701 atcgatggtt cgccctcgcc catggccatg caccaccgtc ccttcgacct cgggagcccg
    20761 cccaagcaaa agcgccgccc ggtcgacatc cccgtcgcca agcgccttgc gaatcagcgt
    20821 cgatgagcag atcagcgtat cgccgctcgc cgtcgaaacc gtgatcgcgc caagctgcgt
    20881 caccacggtt ccgtatcgca ccaaaagctg ccccaaaagc gccgcgtccc cagcccgtcc
    20941 ctggccaaaa cgaaagtcgt gaccgacgca gaccgcgtgc ggaggtggac ctgacaaaat
    21001 gatccgctcg acaaattccg tcgctgaaag cttggcaaac tcggcatcga agcgctgctc
    21061 gatcacatcg tcagcgcccg ccaagtgcaa aagttcccgc ttgcgctgcg acgacaaaag
    21121 cacctgtttg gcattttgtg gcgacagcac cgacgctgga tgcggaaaaa acgtcagaac
    21181 gacgacgcga cccgaagcac cgatgcgctg ccgtgcttcg gcgatcagcg ctttgtgacc
    21241 gcggtgcacg ccatcgaaat tgccaatgac cagcgcgcac ggcgtcaaag acgagctgga
    21301 agcggcgaac tcctcgtagg aggcaaagac tggcatggta tcgggcggaa gtaggcaccg
    21361 ccgggcgggc ggtatagcgc ggagcggcgg cgatgtctag gagcgacttt gcccgaaacg
    21421 ggcgcaggcg agaaaacgcg gggcttaggc ggcggccttc ttctttttct tctggtaaag
    21481 gaagtaccca ccggcggcca tgcccacgaa caccagcgtc aacaggatcg ggctgtgcgc
    21541 gatgatcgac agcaggatgg tcacaaacga cagcaaaagc gcgattggga acatcgccac
    21601 cgcgatcagg aaccgccccg ccgaacccag gaacggaatc acatcaagca gcgtgttgat
    21661 cgggccaaag aacagaatca ggccaaacca catcatcaag aagccgacca accgaagcag
    21721 ccagccgacc gtctggtatt cgctgtgcat cgtctgaatc gcttcttccc gcgagccagc
    21781 cagtgcccgg aacagcgtcg attccttgtc tttgtaggcg cggacttggc cgccatcgag
    21841 cacaccgaac agcgtcactt tggcccctgg tcgcagcgct gaaaagctga tccgcacgtc
    21901 gccaatttcc ggctgcgcga gcgtccccga tcccttgaac agatacttgt cgtcgacgag
    21961 cgagaagctg accggcttgc gcgctgcgat cgcgtcggct ttgcgttcgg ctttgccgac
    22021 ttccgcaggc agaggcttcg gcttcttcac cgaacgaccc ttgcgcttct tctttttgtc
    22081 tttcttcttg ggctggtcgt cttcgtcggc tttgtcttcg gccttgtctt ccgccggctt
    22141 gaccgcgtcg ccttcggctt tttccccgtc cgccggcttg gcagcatctg ccggagccgc
    22201 atcggtcttg gcctcttcgg aactgccctt gaccatgtct tgcgaaagct tcagcggctc
    22261 ggcgctcggc aggctcgctt cggccacctc gaacggatag gccgcgacct gggctttctg
    22321 cgccgaaaag ctctcgtctt tgatcgtcag cggaatgttt tcgtgacctt tgggctgctc
    22381 catttcattg gggagcttcg gcttgtcggt ccagcccttg gaatacgtga ccgtccgcgt
    22441 ctcgactttc ttgccgcccg tctttttttc cgtcgacgtt ttgacggtct caatccaagc
    22501 gaacatctcg acgttgcgat gcagccgcag gtagttgccc ggattgagca gctcgggatc
    22561 gccgatcttt tcgtccgtct tcagatcgcc cgtcaccgaa atgaacttgc cattgagacc
    22621 gctgtcgacg ctggctgggt ttgcgaccac cgattttttg gcaacttctg acaggtcgac
    22681 gcgcccctcg ttcatccaca gcacgatgaa cgaacagata aacaggagcc caccgacggc
    22741 aactcccttg atggaatcga ccaggttggt tcccaaaccc ttctcttcta ccgacgtgaa
    22801 ctgatcagac attggttcct cgatggcgca agaaaagact ccaccccacc gcccccatcc
    22861 ccaaggacag ttcaaattat cacagccgct tacggcgcct caagcgaaca ccggacacgc
    22921 gcacgccgtg caagtcgctg ctgtaacttt cttttcgcta tgttattgta agaacaggca
    22981 acattttttc gcctggagag gttccatgtt gcgccacacc gcaaaccgca agccagcatt
    23041 tcatcgccgc cacctttcca ctgtttcccc tgcggcaagc tgggattgtg gtccacagcc
    23101 cctccagctc gagctgtcgc tcccacccga gcagaggcag tccgcccaca gcacgagcga
    23161 aaagctggct ggttcgtgcg tcctcgtcat caacatcgcc ggggacgacg aagagacgga
    23221 ctagcaagga tcggcgatcg ccggcttgtg cgcaggtggc agccgtccct gcacaagctc
    23281 ttgccacagc gcgagaacct gccgccgcaa gtcgtcaggt ccaccctggt tgtcgacgat
    23341 ccacgtcgct gcgctgcgcc gagcactgcg tgatacctgc gaacgaatcc gcgcctgcgc
    23401 ttcgtccgcc gaaagcccgc cgcgacggac cgcacgctca acttgcagtt ccgtcggaat
    23461 atcgacgacg agcagcccat ccatcccctg gtagatgttg ttttcgataa gcagcgcggc
    23521 ttcgtagacg atcactttgg cgccttgtgc cgtcgctgtt tgcatctgtc gggcactctc
    23581 tgctgcaatc gccggatgcg tgatctggtt taggattcga cgcgagcttt cgtcggaaaa
    23641 cacccgcgcc gccagctttt tgcgatcaat cggcggctcg ctgcctgatt ccgtcacagg
    23701 ctcggtcaga atgcccttcc caaacgcttc gacgatcttg cgataggccg gctggccggg
    23761 caaaaccaca tcgcgcgcca cttcatcggc gtcgacgacc gccgccccca gttcacgcag
    23821 gattttgccg acggtgctct ttcccgacgc aatgccgcca gtcagcccga tgatgaaagg
    23881 acgatccatc gtgcgttaca gcatgcagcc aggggccggc gtgtcaacac agaccgacgg
    23941 gacacagccc cactgctggg tgcctttgtc ctgggtgcac ttggcggcat cgccgcaggt
    24001 attggtgcca gcgtcgggga cgcacggctg cgtgcaaaag tacgtcgggc gggtcggatc
    24061 ttgcaaaagc tgcgaacaga ccgtcgcgct cgtgttggtg cggcactcgg caccggcagc
    24121 gctggagcag tattttccga cgcccaggct gttaccgacg tcgcccggct tgccgcagca
    24181 ggtgcgtgtc gccatgtcgg tcgattcggc gggcatttcg gtggtgttac aggatgaaac
    24241 gacagcaaag acgctgccag cgaggacgga gacgaggaac aaagaacgga ggtgtttcat
    24301 ggctcgcgga gcgtatcaac gcaagccgaa gagtcaaaca gcttggtcgg ttttgcggga
    24361 cgagaccgcc agcgagctac tttgcagggg cagccggagc cgccggcgga gccgctggag
    24421 ccgtcgctgg agccgctgga tttgccaccg gcgcagtggc accgacagca tcgaagttta
    24481 tcccgagcag caccagatcg cttccggcaa agaaaaagcc cgcttcgcgt cgcacatttt
    24541 ccggcgaatc gctgccgtga accgcattct ggctgacgtc ggtgccaaac ttcgcccgca
    24601 gcgtccccgg tgccgctttc ttcgaatccg tcgagcccat caggtcgcga tatttcttga
    24661 tcgcattgtc cccttcgagc accagcgcga cgatcgggcc cgacgacata aagtcagtca
    24721 gtccctcgaa gaacttcttc ccggcatgca cagagtaaaa ggtctttgct tgatcctttg
    24781 acatccgcgt catgcgcagc gccaccggtt tcagtcccga cgacgccatg tcctggatga
    24841 tgtgccaggt gtgacccttg ctcacagcat cgggtttgac catcgccagg gtgtattcga
    24901 cagccatttc tacgtctcct tgtcgtagag attgtgcctt gtttggcagt cccttagacg
    24961 gcgattttga accactgctc gccagaaaga aagggtcatc gagcctgcgg aaaacgacgg
    25021 cacggcgccg cgtagagact cacatgtcgc ccgctggttg cgcggatatc ggactcaacc
    25081 agtgataaca gagcagtagc aaaatctgat ttagtccaaa acactcgagg ctcaaacaga
    25141 gcgcatcctg gctgccaatc tgatacacgt cgtagtgctc ttttccgatg cagacacagc
    25201 ccaaatgatc actttcgcgc agagccagga ctgcctgctg gattcttcgt cgacgttcac
    25261 aaattttcag cagtttcgga tcgactccga gcgcgcgcag aatcggcagc gcgtcctcgg
    25321 caggaagcac catccagtgc ttctgcgcca ccgcctttat ctcctttagc gatctcagcg
    25381 gtgacctttt gaccaccatc tcgacaagct ctcctcagaa ctgtttggac tgaagttttt
    25441 gtttggctca aatccggtcc attgggaaga atttttccta tcaatcgcca cagcttcgtc
    25501 gaaggcccga tcgcgcaacg accgtatatt ttttatacac aaaatcacta tgtaatccga
    25561 ctcacacaat cgcttgacac ctttggctga tcgtccgaac actgcgtcgt cgttcggtcg
    25621 gcatttgggt gttttcgcga tgtcaaaaac ctcttcgaca aaacctcgct cagcgcagcc
    25681 ttcatcggca cagacgaagt cgcctcggtc ccgcagcggt acccagccgc ccgtcgaacc
    25741 caagcgctgc cttcgctgcg gcgccaaagc ggtcaagccg cgcctaggcc ccggtcgcac
    25801 gacgcgctat cggacgatgc cgacgctggc catccccgaa gacgtagcga tccccgcttg
    25861 cggtcgctgt caaagcgagt atctcgacga agaatcagcg ctcgcgatcc agcctcggct
    25921 gcaaacggcc tatcttttgg cgctgcatgt acggacgcgg ctggcgattg atgtcctgtc
    25981 gcagcacatc tcccagcgac ggcttgaaca gcttctgggc ttgtcgcagg gctatctgtc
    26041 gcgaatccgc gccggcgccg gcaatcccag ccccgagctg gtcagccacc tggcgctgct
    26101 cgcgcaagat ccgaagtcac ggcttttcga attggaacga ttttgggcgc ttcccgatga
    26161 caactggctc gaaaccgcgc cgcgccccac ccacaaacgg acccgcctgc gccccgagcg
    26221 cgacgtagac cacgcctaca gcgattgacc ccgcccaagc ggacgtttta cgctcgccgc
    26281 acatgttgct tttgtctgcc ctcgcctttt ccattccatc gcctggcggc aaagagctgg
    26341 acctgtttca gcttgtgctt agctcgggtg gcgtcgtcct gttcgtgctg tttttgctga
    26401 tcgtgttctt cgtcgtgacg acgttcgtga tcggctacaa gggctaccag ctcgtgctgg
    26461 ccagccgcga aaccgaccag tttttggaca ctttttggca ggtcaaacgg ctggaaaaag
    26521 tctatgaccg ggccaaagag ttgcggcgat cgcccgtcgc tgcgcagttt cgcgccggct
    26581 acctggaact ggtccgcctg accaaaaagc gcgacgaggc cggcagcgaa ggcggcggtg
    26641 acgccgaaag cgacctggaa aacatcgaac gcgcgctccg ccgggccgcc atcgccgaga
    26701 ccacatcgat ggaagcgctg acaccactgc ttgccaccgt cggttcatcg gcgccattcg
    26761 tcggtctgtt tggcaccgtc tggggcatca tggacgcctt ccgcaacatc ggcagcaaag
    26821 gcaccgccaa ccttgccacc gtcgctcccg gcatcgccga agccctgatt gccaccgcca
    26881 tcggcctggt cgccgccatc cccgccgtca tggcgtttaa ctatttcgcc cgtcgcatca
    26941 aagtgctcgc cggcgaaatg gataccttcc acaacgacct gctcaacatc atcaagcgca
    27001 actacctcaa ctagcggtcg ctggctttcg atgaaacgtc ccgacgggtc gccgattctg
    27061 tcgtccatca atcaatcaat cagcgccggg gcgctcacat cgacttcatg cacagggatt
    27121 ttttgggccg ccagcgacga tagcagagca tcgatccccg acgagtcaag cggtagccgc
    27181 gtgatctcgg cgccactgtc agcaaacagc cccagtgcgt cgtcgagtag cttcgattct
    27241 ccccagttgc tgttgcgcgc aagccgtgcc cgcacgaccg aatccagccg gtagcgcccg
    27301 cccttcgttt cgtgttcgac gatcagaaag ccgtcagcca ccgccaccac atacggcgtc
    27361 gcgctgcgag ggacaagtcc gcggtgtttg gcccaaaaat accaggggat gagcgccaca
    27421 tacggaacca gcggaagcac aaacgacagc gcaatcaccg cccccgaaag tcctcgcaac
    27481 caacccggca ccccatccac aaagtacagc gccagcacca caaccccaac acccgtccca
    27541 agcgccaaat ccccccacgt ctgccgtgac aaacccgcca tccctcactc ctaacacacc
    27601 gagggggatg gggggtaaag accaggatgg cgcgttacgg ccaaattccc cgatcgccgt
    27661 aagcccggcg aatccgatcg agcccaatcg catacgccgc cgtgcgcaga tccaagcgct
    27721 tttcatgcgc aaagtcccgc acccagccat acgaccgctt catctgcttt tcgagccgcg
    27781 cctcgacctc ttccagatcc cagcgctccg agcgcttgtt ttgcagccac tcgtagtacg
    27841 acaccgtcac accgcccgag ttggccagga tgtcgggcac caccgtgatg ccgcgctcgt
    27901 tgaggatcgc atcggcctcg ggccatgtcg gaccgttcgc gccttcgacg atcagccgcg
    27961 ccttgatgtt gcgggcttcc tgctcgccga tttccagctc gagcgctgcc ggcaccagga
    28021 tgtcgcactc gacggcgaag aactcctcac ggctgatcgg cttcgagtgc ttgtagtcga
    28081 cgaccgatcc ggtcttgcga acgtgctcgg cgagcttgtg cgggttcagt ccttcaggat
    28141 tggcgatgta gcctttgtaa tcgcccgtcg ccaccagcga cacaccgagc ttcgacaaga
    28201 ttttggcggt gaacgaaccg acgttgccaa agccctgaat cgcgacggtc ttgccggtca
    28261 ggtcgaagcg gcgatctttg gcccactcgg tgatgcagtg gacgcagcct tgacccgtcg
    28321 cagcttcccg cccaagcgat ccgcccgagg tgatgctctt gccggtgaca acgcggcgca
    28381 tcgcgttttt gtcgagcgcg ccggcaatgt tcatatacgt gtccatcagc cagttcatca
    28441 cttggctgtt ggtgccgacg tcgggcgccg ggatgtcgta ttcgggccca atcgagctcg
    28501 ccagcgcgtg tgtgaagcga cgggtgatgc gctcgatttc gccgatgctg tgcagcgtcg
    28561 ggtcaaactg cacgccgcct ttgccgccgc caaacggaat gtcatgcagt gcgctcttcc
    28621 aggtcatcgc cgacgccaga ccgcgcactt cttccaggca aacatcctgg tgatagcgga
    28681 tgccgccctt gtacggtccc aggatgttgt tgtgctgcac acggtagccg gtaaacatct
    28741 ggtacttgcc gttgtccatg cggaccggga agtgaacgat gatttcgttt ttcggctgtc
    28801 cgaggataag ggcaatctct tcggggaggc tgatcgcctt ggcggcgcgg tcgatgtgct
    28861 gattgacgat cgcgcccagg tttcccttgg gatgttgcgt cgcggtttga gctgccggcg
    28921 ccgggacagg ggtcgaagag gatttttctt ttgacatgac actttgttcc ttggctagtt
    28981 ccctgggcgc gccgacaggc aaaacacgtc atcggcgaca gccagagtgg aatatgagtg
    29041 gaccaatcta ccccaaagca gctcgtccag aaagtttcgc tgatgatgat tttctcgacg
    29101 ctgcgcagca tataagaagg ccagaccaca cagccagaaa cagttccgtg acagcggatt
    29161 ctctgtcggg aagtttgctt ccccacaagc tgtggatgga accttctggc ttggccgggc
    29221 aagctgtggt gcgaatcttt gacgcagtgc agcagacagc gcgcagcaaa ggttggttcg
    29281 cggcgagtgc actggtgggc cgcagcctgc gcgatgatcg caaacttcac tcacgcgagc
    29341 ggcgctatgt cggcgacacc gtgttcgcca tgctgcgcaa acaacggcgg ctgcgggcac
    29401 tgtgtggtgg caaaaatccg tcgggccaag cgatctacgc agcgtttttg gtcgatgaac
    29461 agacggcgca cagcgatgcc gaactcgatg ccttgtcgcg atttttgcgc agcgccggac
    29521 tgtcgctccc cgacgtcaaa gtacgccggc aagacatcga acagcggttt tccgacatcg
    29581 cgtcggccag tcccgatgcc caggcgacgc tgatcggcga agcgctgtcg tatcccgact
    29641 ggctcacgtc gctgtttgtg tccgagtttg gacttgcgca ggcgatcgcg atcctgcacg
    29701 cgcaaaatgg acgggcgcgg ctgaccttgc gggccaacac gctgcggtgc agtcgcgacg
    29761 agctgtgcgc caagctgcac gcgctcggga ttgccgccga gccgacgccc tttagcccgt
    29821 ggggagtggt gctcgacacg cacctgaacg tgtattcgct gccgctccat caggaaggtt
    29881 tttgcgaagt ccaagacgaa gcgagccagc ttgtgacgga gctgtgcgca ccgccgccgg
    29941 gtgggctggt catcgatgcg tgcgcggggg ctggcggcaa gacgctggga ctttcggcga
    30001 tgatgggcaa ccgtggacgc attttggcgt ttgatatcga cgacaaaaag ctcagcgaac
    30061 tgaaacagcg cgcgcgacga gccggtctta ccaacattga aacccggtgt cttcgcgaag
    30121 acggtctgct tcaccttgtc cccgagcgaa tctgcggtgc cggggcgcag cgcgtgctgg
    30181 tcgatgcgcc gtgttccgga ctgggcgttc tgcgtcgcaa tcccgagtca cgctggcgac
    30241 taacccctgg cgacatcgac gaaatcaccg tcaagcaacg gcagatcctc gccgcgagcg
    30301 cccgcctggt tgccccacac gggcggctga tctacgcaac gtgtacaatt ttgcgccgcg
    30361 aaaatgacgc catcgtcgac gattttctgg ccagccaccc cgacttcgta ccgatgccag
    30421 tcaaagaaat cctgagcagc agccgcgccg ccccgcttgg tgacggagaa cgcatgcgca
    30481 tctatccgac ggaaaacggt cccgacggat ttttcgccgc tgtgctacgg cgcaaggccg
    30541 actgaccccg cttctgattc ttccagacgg ggcggcaccg caacacgcgc gaagtcttcc
    30601 gccgcgacga catccggaaa aatcgcagcc gcttccgcca catgccctgc cgtcgaggta
    30661 tagcgctgtg aaaagtgggt cagcaccagc ttgcgcgcct gcgcctgcac ggcaatctgc
    30721 gccgcctggg ccgccgtcat atgcgcaaag gcatgcgctt cttcttcgtg ctcacgggta
    30781 aacgtcgctt cgctgaccag cacatcgacg ccacgcgcca gctccaccgc ctgggcacag
    30841 ggccgcgaat ccatcaccac cgcaagcttt tgcccaagcc gcggcacgct gacttcaccg
    30901 atggaaatgg tcttgccgcc cacgacgatc gtcccgcttt gctgaagctt tttgatgtcc
    30961 ggcccgcgca ccgcaagctc agccagcttg tcgggcagca tccgcacacc gtcggcttct
    31021 tcgattcggt agccgaaaca ctcgacgcgg tgcgacagcg gacgggccca aattttcact
    31081 ttcgccgcag caatttccgt cgccaaaagc ggtcccacag ccggattccc gtcgtcgccc
    31141 gtcggcagtg ccagcggcac cgggacaagc tgggcctgct ctttgtaaat cgaggcgtag
    31201 cgcagtcgct cgaaaaacac ctggccgctt gcgggataga tgacttcgac gggatgcgga
    31261 actttgtcga gtgaaattcg ctgtgacaac gccgccagac ccaagcagtg atcgccatga
    31321 aaatgggtaa tcaaaatatg ggtaatctgc gtcgctgcaa tcccggccag cagcagctgc
    31381 cgctgcgtgc cttcacctgg gtcgaccaag ataccgagcg tatcccagcg aataaacagg
    31441 ccattgtggt tgcggtgccg agtgggaact tggctggcag tgccgagaaa agtgatctcg
    31501 cgggcggaca tgcgcccccc atattgccaa accttgctgc tttgccacga aagcatcttg
    31561 agccgcggcg gctttccgaa ggaaaatggc ttgatacgcg cagtgttcgc ggaggtgcca
    31621 tgcagcgacg taaccgccac cagcagacct ttcttgctct ttcgctcctg cttttcgcct
    31681 gcgggaaaaa ccccgagcag aaagccgccg aaatcggcaa agaagtcgac gcagccctga
    31741 gtacgcagca ctgggaacaa gcggaaaaaa aagcgcagga aattctcgcc atcgccaaaa
    31801 tcagcgacgc gacccgcgat caggccaagc tcaagctaga gcaagccaag agcgagcagc
    31861 tggccaagac cctgtaccaa aagtttagcg gcgccgccgg caccgatcac gacactgcga
    31921 tggcggcttt ccgcgacatg gcgcagggat cgtactatcg cggacttgcc aagaccgaat
    31981 acgagcggat tttgccaagc tacatcgaag accacttgga aaaagccaac agcgccagct
    32041 tcaatggtcg ctgcgccgac gccaaacagc agctacagat ggtgttcgac gtcgatccgc
    32101 agaaccaaaa agccgtcgag cttggcaaaa aaccctgccc aaaaaaagca gactagcccg
    32161 ccgcccagcg actacggaac ctccgcaccc ggcatacaga gcgtgccaac acggttcacc
    32221 ccgaaaaagt cgagatctcc tgcacagctc agcttcgcag caccgatcag cagcagatcg
    32281 gtcagtccac tcgcgacaat ctgcccaccc ggatgggagc gactgatcgg atggttaaag
    32341 atcgcggttt ttgccaccag ttcccccgga atgggcgagc taggtgcggt gaaagatgga
    32401 caagaggctt tcccggttga tttattcagc tgattattca ccgtgtcgat cacattgccc
    32461 gctgacacgg acccatttag ctgaacacca gttgtgcaat ttacagacag cccatagttc
    32521 actgccgtat tcaccgccgg ggcagagctg atcagtgaat tcactatcag gcttttttca
    32581 acagtaatcg agtatgctcc accagcccca gtcatttgct caaatacatc aatcacaccc
    32641 ttgtactctg taccattata tccactcagc gcttcatttt tcaaaatcgt ggatgaacca
    32701 atcaaaacat ttccgtttac aactgaaata gccgctccag ctgcttgatc ggtggctgca
    32761 agcgtagcgc catttaaggc cgacagattg tttccgtaga agatggaatt gttggtagaa
    32821 agcttgccat tcaagatgaa aatggcaccg ccgcgaatgt gtgctgagcc ttcgacgcgg
    32881 ttgtctagaa aggcaacccg gtcgagcttg atctctccca tcgaaacgat cgagccacct
    32941 gccgcaaccg gtgccttccc ttgggcggct gggtatgcgc tctggtgagc agctggcatg
    33001 tccgtcgcct tgccaccctt caagtgcaag ttttggagcc acagcgagcc gccagaattt
    33061 acaataaagt ggcgggaatt ttgatttgca tgtagcaccg ttggatacat cgaattggca
    33121 gatgacatat tcagcggcag tccatcgatc accgtctgcc gcgtaatcgg agcaaaaaga
    33181 gctgacacag aggcatcagc acgcgaaaat cccatagtac ccaaggccgt tcctgcacat
    33241 gaaaatcgaa tggttacgct gttgccagga aacatagtcg agttttgcga atctcgaatc
    33301 gttgccatga cattgctttg atttgtacca ccattattct ggtctggatt tgaacaacac
    33361 gcgacaagag ttggctctgc cgaatccatt ttataatatt gttttccatt acatggatct
    33421 accaataaat caggattctc agccatgtct gccacatcag acatgggcat gtcttctaga
    33481 acttgatacg tcgtccacag cccactgcat gacgacaagg acagaagaga agcagcatac
    33541 acgtacttgc gcatggttcg acctccatat tctaagacag caattatcag tgatttgata
    33601 attactattt ataaacaata gaaaacgtat cgcgctgtcg cttcggctcg ggtattgcaa
    33661 tcgcaacagc cccgccaacc aagaacacca cgccgatccc agtgagtgcg ccgccggccc
    33721 ccccactatc ccggtagtac ttggtcgagc catccccacc acacgggctg ct
//
LOCUS       DAORGN010000027        33448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_481_length_33448_cov_5.771898, whole genome shotgun sequence.
ACCESSION   DAORGN010000027 DAORGN010000000
VERSION     DAORGN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 33448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33448
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_481_length_33448_cov_5.771898"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="PKL17_05075"
     CDS             <1..2460
                     /locus_tag="PKL17_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44131.1"
                     /translation="VLGKGTYKTDAMGKKIIDDPLMHVVIKDMALDFYVYIDERYVRF
                     MRQTVDLDLPLGLDVDAMNQIVPILGDLAMAVGNVRVSDSSLLKESPAQLARLFPSLL
                     PVLAGSLSGSLSPIKLPDIMGLQLSPVQITSTADPTGKLSLLGMFLSIKLSTMPIAGE
                     DMSLAAQAADEPTETFAKLLSVEVPTAAELAVTATEQKSTRVTLDLDAKSHLSGDREW
                     QYRIDGGLWRPFDGQQRLVIEEPELRLAGPHNIEVRARVAGQPGTLDPTPARVDFVVS
                     PVAAPASAKPTCVGSKSDSSSEQSSGQSMTGCSTSRSDTSRGTLIAFAIALCGLLSRR
                     RLAKVGTLAIAALLGVSQTACNNNPTGTGDGTDKPPVEVKKPKPEYAANDEVGRYQSI
                     AVTADKIYVSAYNSTFGDLAFTEMSMPSNQSTWLPVDGLPTGDPDTMDPSAYRSGYAD
                     AADDVGRFTSLKLNQGGNPVVAYQDVTNSAVKIAARKDNKWSTLVVADQNEGRGVGGF
                     AQLILDASDVPTVAYMVAGNKKSDGKVFAQMVVATAMSASPQSPSDFQKKVVEEVQVS
                     CAGLCGTGEACVYADPAVKDRQKTICKKTESTCTPSCKSGLACVAAKCVDALGTPPSD
                     LPQGTGLFARLVQNQDSRFLVFYNRATGALKMATGTDWKVTTLDGGDGKVDTGRHVGA
                     FAASDGTLHVAYSDADGRLFYKNAKGTMIGKAELVDDGNRTMPTPDVHSVGAGATVVL
                     GDSGPVIAYQDATAGTLEVAQKAMSWTHKTVGAEPGLSRGYYPQLFKWNSKWWVSDVV
                     YDRNADALTTLRFSELSQLTP"
     gene            2500..3438
                     /locus_tag="PKL17_05080"
     CDS             2500..3438
                     /locus_tag="PKL17_05080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44132.1"
                     /translation="MSTISGMSGTSSAMPRPQAPPPPPQQNKFRRDPRLAAVYDRDIS
                     PVWTQPFGKLLLGQVATLPGKTTILDVMCHTGDPGLELLRRFPDSRLVAIDPSAAMID
                     VARQKAGPLLSRRVFLRTEAGEPKLPFDEAAFDLVVSNLGLHESAQPHLLLRELARVA
                     KPGAQIITTLPLRGTFAEFYSLLETELQGRELEQQRLSSHLQSWPDVDVVRGWAKAAG
                     LAEVELLISPFSLLFAGAVDLYYAPLIEFGPLLAWKSLFDRDPAGMQAVFVSLRDRME
                     QLCTQHETPPLGLRPPLRKPLALTVRAACLRARRPL"
     gene            complement(3521..4132)
                     /locus_tag="PKL17_05085"
     CDS             complement(3521..4132)
                     /locus_tag="PKL17_05085"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNK44133.1"
                     /translation="MLDPFYLIVDSFAWLLRLLPLGVKTVQLRIKNQPTDEIIRQTQQ
                     AMALCAQHGATLILNDYWQTAIDLGCDFVHLGQEDLAEADLAALRRANVRIGVSTHTP
                     EELETALRACPDYVALGPIWPTLLKVMKYPPQGLARIGQWKKQIQAIPLVAIGGLTPD
                     RAPLVLQAGADSACVVTDVLTHADPESRSKEWLRATEPFRKKT"
     gene            complement(4125..4922)
                     /locus_tag="PKL17_05090"
     CDS             complement(4125..4922)
                     /locus_tag="PKL17_05090"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNK44134.1"
                     /translation="MQDNVRFYGESFACRLLLGTAQYPSPAILSAAIAASGAQIVTVS
                     LRRESSGSSSGGRAFWSMIRELGVKVLPNTAGCHSVSEAVKTAEMARDVFETDWIKLE
                     VIGHADTLQPDVFGLCDAARELTAQGFKVFPYTTDDLVVAEKLLSIGCEVLMPWGAPI
                     GSGRGLNNRFALKAMRAHFPGVPLVVDAGLGVPSHAAEAMELGYDAILLNTAVAKAGN
                     PVLMAKGFRLAIEAGRLAYMAGAMEPRDMAVPSTPVLGLAFSESKRA"
     gene            complement(4925..5074)
                     /gene="thiS"
                     /locus_tag="PKL17_05095"
     CDS             complement(4925..5074)
                     /gene="thiS"
                     /locus_tag="PKL17_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNK44135.1"
                     /translation="MADLVTELGYTDMPVATAKNGTVVRKKDRDATAISDGDVVEVLV
                     PMQGG"
     gene            complement(5091..6102)
                     /locus_tag="PKL17_05100"
                     /pseudo
     CDS             complement(5091..6102)
                     /locus_tag="PKL17_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406320.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(6233..6811)
                     /locus_tag="PKL17_05105"
     CDS             complement(6233..6811)
                     /locus_tag="PKL17_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNK44136.1"
                     /translation="MFGSSLLIAAALSGAPLKLSAQAGEDRSWLSAVVVGALLETQRA
                     AQTEPVLGFANGQPQPLLIVDMPSPYAQTQVRAEVHTALAFYRMQTAAQRFGLSLFLN
                     SGFRTNQQQQDVFDLYRRGRGPLAARPGFSNHQSGHALDIDMRLPRVRLWMSRHAHEF
                     GFQRTVPSENWHWEFRGSVQRLLRQPVAPGNS"
     gene            6947..8728
                     /locus_tag="PKL17_05110"
     CDS             6947..8728
                     /locus_tag="PKL17_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNK44137.1"
                     /translation="MGRNFPNTDRRAEAGRVAFGPTRWASRVGGLLALGLSACIEPTS
                     APPEKTPVESARPIPGLTATPHEQIVAGHLLVEFRRGATPPIVQPPSPATLLPPDENP
                     ATVVLADERLILERELWPPPASDSPLGQRDPQAGQIVLLRRDGIDESATLALLDKVAS
                     DPQVRRVEPDRLRHATSVPNDTLWSSQWALPLIRMPRAWDISTGSPDVVVAILDTGII
                     HDHPDLESRLIPGYDFISRPASADDGDRVRDSDPTDTGTVDTSRLHGSHVAGIIGAVS
                     GNAMGIAGVDQKCRLLPVRVLGVKNGDGIDSDITDALRWLTGAQLGTIPVVSRKVDVV
                     NMSFGGPGLSFTLQRAVDEVISAGLIVVVAAGNGGTDTTTYSPGGLDNVISVGASDAK
                     GKRAVYSNYGPRVDLLAPGGGLSEWDYETDLAGLPPDGILSTYRDEGVAEPKRPPFTY
                     SPLTGTSQAAPHVAGIAALARSLVPTVRQRTMGLLLRTAANAKYRCASDDLSGCGAGL
                     VDAESTLLLTQLQKRCGCEGDLYCVDGTCQTPPQIHDSIWDRPLVRGGYCQLGLSSEP
                     AAGPWLGLFTAALVLLLLFALKARPKA"
     gene            8725..9921
                     /locus_tag="PKL17_05115"
     CDS             8725..9921
                     /locus_tag="PKL17_05115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNK44138.1"
                     /translation="MNTDDVVVTGLGVVSGLGKDLSAFCDGLFSGRSGIGPLSLFSCP
                     DARSQLVAQAPDPDLSRILLPGCTSRPDRFGIAAALAAVEHAGLSTPDLHDAAVLFGT
                     GTGGAIETEQYLRELQDGMLPSPKLLVTHQPASVTDLVARILCAYGPRTTVMTACSSS
                     AIAIAQALDWIRLGRCEVALAGGAEALCKLTVAGFGALRATSPEPCQPFDKNRKGLNL
                     GEGAAVLVLESRRHAERRGAKVLARLLGAGMSCDAHHMTAPQPEGIGARQAMEAALCD
                     AKVGREAIGYINAHGTGTPHNDAAESRAVRDLLGDRAPHVPMSSIKSMCGHTLGAAGA
                     IEAAASVLSLVHGVLPPTKNLRDPETEFGLDYIPEVPRQKQVDVVLSSSFAFGGNNAA
                     LLFGRA"
     gene            9968..10387
                     /locus_tag="PKL17_05120"
     CDS             9968..10387
                     /locus_tag="PKL17_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44139.1"
                     /translation="MKETVRTSLGEIYREPDSPLVVLRRSSTPALDEDELLAMLDRFD
                     AILPPSRRRTLVFLMDMRDGPMRNDEEYEQRMNHAMQRLCAGFARVAVLMRSSVGILQ
                     ASRLRREHPIVAGTVRPFLDETQARSYLLTSSEKPSP"
     gene            10453..11148
                     /locus_tag="PKL17_05125"
     CDS             10453..11148
                     /locus_tag="PKL17_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK44140.1"
                     /translation="MSKLAEIVQRLRKWVSKKGPAQPVVPDESPVLRQKREPLFRCAM
                     QILDDAAGAEQVIRQALREAERGVMAPNGPAPPKHDEWLLRQVVSLSVQRLKALSIVP
                     VVPPLPDAARGGSESESDGADPSATQGRSTSEQQRRERLSRALLKLPVEMRVTLILAR
                     MQKRPLTEVATLLGTTETTCLFWLSHGRKQLRRALQRDLAPGPENGGPLRILVSPETP
                     HDVRGNKKAIARA"
     gene            11135..11428
                     /locus_tag="PKL17_05130"
     CDS             11135..11428
                     /locus_tag="PKL17_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44141.1"
                     /translation="MPEPEPVHVASVSEHLASCPPCRVEQEALREVDRRLEQLGEHRL
                     AAAAQVLAKTRGPSPHTPILPTQPTIAVWKMVGAAVLGVLILFGTLLLFFRGK"
     gene            11470..12594
                     /locus_tag="PKL17_05135"
     CDS             11470..12594
                     /locus_tag="PKL17_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNK44142.1"
                     /translation="MSKDPTESADKHDHDWIDIGSADELAKTPLQQIMLGSTRIALSY
                     AGGVFGAVSGVCNHAGGPLGKGTLDGDYVVCPWHQWKFHRVEGQGERGFEEDVVPAYH
                     VRVRDGRVEVSPKPHKKRAKKPHAPHPLSRTPERQPGRIRVVGISTTAMQKDFPRYST
                     SDALLEVAMDHAKQAGYDTQMIYLRDLSFRHCEGFYSKAARACTWPCSITQMDPNDQL
                     DRVYEALVHFGDVFLIATPIRWGAPSSLYFKMVERMNCIQNQITISGKALLQNKVVGA
                     IITGGQDNVQAVAGQLLGFFGELGCQFPQFPYIAHSRGWTAEDMERNIDYVKQSEELR
                     QAARELVDRSANLAERLIATTPPPVPRGGRKGRSLPLVKE"
     gene            complement(12595..13212)
                     /locus_tag="PKL17_05140"
     CDS             complement(12595..13212)
                     /locus_tag="PKL17_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44143.1"
                     /translation="MKKQLLVVCGVLALSLSVASADGNADLIQRSYDSEAVGKLQDAL
                     TALDQMSSPRRDGYVVTLRKAWLLYRLGRSAEAVEVYGRAIAQAPAAVEPRLGILLPQ
                     MALRRWGDIETQSRAILKLDPANYLATLRLAFACYNLQRYAESAAFYKRLADLYPGDV
                     EVKVGLGWALLKLGKYAEAAAEFRTVMDISPRNELARDGLKAAGG"
     gene            complement(13209..15362)
                     /locus_tag="PKL17_05145"
     CDS             complement(13209..15362)
                     /locus_tag="PKL17_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNK44144.1"
                     /translation="MRLWGFIRSLLLSYAQILFSGSVWVGLLLLVATLAMPIAAGYGL
                     LCAGSALLCAKLFQLEPEERKSGGYGYNALLCGLGIGHTCVVGPGSLALAVFCGALCT
                     VVAAGLRASIGRLHLPMLSIPFIIVYHLVLAVVEPAGLKAPPVTELVDSDGPALLRGL
                     GALFFLPRAEVGAIVLFAILVHSRISAMLAAMGFAVAYLFGIYFLGLPVETIVLVVSY
                     NAAFVAVALGGVWFVPSASAWLLGLFGSLVATLLCAGLLPIHNRLGVPIMVVPFNVTI
                     LMILLALRQRVSDSHPKAVDVMLATPEDNLEYLRARLLRFGWSQPVKMRLPLRGAWIC
                     TQGVDGAHTHKDRWRHAWDFEVLGPDGKAFRGDGSKPDQYYCFRLPVLSPAPGTVVRI
                     ENDIPDNLVGTMNLKQNWGNLVLIHHGVGIYSLLAHLSRGSVKVQLGQVVQAGDVIGL
                     CGSSGRSPQPHLHFHLQASPDIGAATIPCSFADAVVIDADRQLLHSALIPVEKQQVRN
                     LEPDEDRGSFFSTLAPGLGGEWRFQIGDREERILLEVDLFGQPLLRSLTYGTVLRYVL
                     TDDLFTTYDVSGSRRSVLYLLRTALPRIPLESSETLRFRDLLPGRLVRPLGLQLLHDF
                     VAPLRARDGIEVELCARRDGQLLQVDGQSLVDGDAGASEDGKKRGGTDTEPDLRTRVL
                     LARGVGVVRIEVTNRGRTVRAERITAPPTTKEETR"
     gene            complement(15429..16775)
                     /locus_tag="PKL17_05150"
     CDS             complement(15429..16775)
                     /locus_tag="PKL17_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNK44145.1"
                     /translation="MSKLPYERPTLVRHQMGQMNKFGRLQGMRPLTHIDGVAVSDLVA
                     KYGSPLFVFSQKTLINRYRELQQAFSRRYAKVRIAWSYKTNYLEAICRTFHKEGSWAE
                     VVSPFEYEKAIRTGIVPDHIHWNGPYKPDAAIERALSGGAMIHIDNFDELSRIERIAE
                     KMGIRPKVALRLNLAIDGLPAWSRFGVNLESGQAREAVNRLVSGDRLELTGLHSHIGT
                     FILAPESYGQAAAKLARFANEIRAAHGIKLSFIDLGGGFASPNTLAAQYLPGEQVSPS
                     FERYADAICDGLSLLEYPSKELPTLVLETGRALVDDSGYLISTVEATKRLPDGRRGLV
                     LDAGVNILFTAFWYKHDVVPAQEFGGLLEPTVLYGPLCMNIDVLRDTVQFPSVSPGQR
                     LVFRNVGAYNVTQWMQFIAMRPAVVMVSTDGQVGLIRRAEGLDDLAALEEVPAWLK"
     gene            complement(16772..17821)
                     /locus_tag="PKL17_05155"
     CDS             complement(16772..17821)
                     /locus_tag="PKL17_05155"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNK44146.1"
                     /translation="MSEKPLTVAVTGLNATDNPAPGVAVVRALRDAFPKARIVGLAYD
                     ALDPGIYATELLHEVFMLPYPSQGLTAFWSRLEYVHSKVGIDVIIPTLDAELPAFLDL
                     EPKLRNLGIRTLLPTREQFTLREKGRLPQLGQLSGISVPATATVNTIEELNRVHQRVP
                     YPMFVKGLYYGATLAHSLDEATHAFYKVVAQWGIPVVVQTLISGEEFNVVALGDGEGG
                     LVGAVGMKKLMLTDKGKGWAGITVRDPALLDVPRKFMAATKWRGPCEIELMRDKNGEY
                     HLIEINPRFPAWVYLSAGAGMNLPQACVELAMGKKPSIASDYQVGTLFVRISIDQIAR
                     IEDFQNMVTTGEVSR"
     gene            complement(17830..18096)
                     /locus_tag="PKL17_05160"
     CDS             complement(17830..18096)
                     /locus_tag="PKL17_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HNK44147.1"
                     /translation="MSGAHLKDLALSDTGFVFDPYTGATFTTNAAGLAILHALREGLS
                     RPAIVARLRERFDVHGDVESDLAEFFQLLRQHGVVPQNFTVAEK"
     gene            complement(18105..19373)
                     /locus_tag="PKL17_05165"
     CDS             complement(18105..19373)
                     /locus_tag="PKL17_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44148.1"
                     /translation="MMRRAALWCLGFVLGGAPQLAFADAYLDASRVATELETQGRLDA
                     AAEALEAIAPSYPQDLDLALRMAWLRFRAESYRRAFSHYETALKISPLSIDGHLGMGW
                     TLERLSRCDEARAHFLTLATQNPNHAGAKEGLLACPEPAGFALNTSAFLTGMAYAGHY
                     ERQGGIAVSPRLELVIDGKWLIGAAYRYSEFFLQTQTQTQVTTPGMPPRPGVPPMPPM
                     TTTVTTISSTTFDQHEAYLHAGYSGTRLSLIARYAFLVDSRGLSQASHHAGLGLRISP
                     LGLGDAQLQFATSVYSDLTIVRLAPSWRLPIAKGFSVQLGGVGQWASGSLLGNGFLEF
                     SYVRGRIAAYAGGSYGEQLRPAQLELSVITNMYERVAGSAWAGVSIRLPRDFRLLVDY
                     RFDRLRKLSGVARSTDSSGNYFTLGLSKAF"
     gene            complement(19586..19804)
                     /locus_tag="PKL17_05170"
     CDS             complement(19586..19804)
                     /locus_tag="PKL17_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44149.1"
                     /translation="MTPLEPRLVPLVDQLRNTIAEMEKLTTQARSILAQIDGSEPGRD
                     EILAERARRAVMELRAKVAATKRTQQKG"
     gene            complement(19832..20686)
                     /locus_tag="PKL17_05175"
     CDS             complement(19832..20686)
                     /locus_tag="PKL17_05175"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNK44150.1"
                     /translation="MTRRPLVFARRLFAIASLSVSLLTAACGPELAAIDEMEGDNLPF
                     HVEPQSIPTGYYDGTSGKSGNDLLVALSDIVSRGTRSLSYNAARDKMFADAEDPDNDD
                     AVDCVYTGRIGKPVNSTSTANSAKMNTEHTWPQSLGARGVAQSDMHHLWASDVDTNGR
                     RANYPFGVVKTVTWTAPNPDGTSPSKLGKDDSGRTVFEPHDKTKGDIARAIFYFYTRY
                     YRSRPADFTLNNFNVEEATLRKWHESDPPDADERAHNDLVYAIQGNRNPYIDRPELLK
                     QIPDFPDR"
     gene            20803..21438
                     /locus_tag="PKL17_05180"
     CDS             20803..21438
                     /locus_tag="PKL17_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNK44151.1"
                     /translation="MKVVFNLLQCLRNAPLRALVLLGGLLLIVGIFPSVAHAQTSYFT
                     PRTILAEFFPRSQAVSFERFDLTAEQRQKLTEVLGYTPRKPSYTIYIAKTGDQIDGYA
                     IIDEEKGQHLPITFAVQFDAKGAVKRQELMVYRERYGDEIRDARFRQQFVGKHVKSQL
                     REGEEIIAISGATISSRAMVIGVRRALVLLDELVLKRPMKQPDTAKAIPSS"
     gene            21460..22110
                     /locus_tag="PKL17_05185"
     CDS             21460..22110
                     /locus_tag="PKL17_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44152.1"
                     /translation="MRQFASSRFRLWNLSPAAKLIYTFFCGLSLLAIVSSVLLYEDLV
                     GPSLRSGHVEHVERYYGKDPAKAVPAQPAQKAEQPVGGPAIALPEDEATVQLEVAMPY
                     RKLLEVTHFHLFTIPVFVLILTHLYLLTDAEALGQLVWISLAWLSALLHILAPWLIRF
                     GSRHLALSYPLSGFGLFVACGWLTVYPVYVMWRGKAPAPSEPTPPKKAEVAPPFSD"
     gene            complement(22111..22866)
                     /locus_tag="PKL17_05190"
     CDS             complement(22111..22866)
                     /locus_tag="PKL17_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNK44153.1"
                     /translation="MHNIRNAALCTVSLLALGFQSGCGAELESTEPVNPSFFEETAMP
                     ACGTALASWNGTTAYSNGGNTGTGVSCAGVGSYGYQYQCVELVMRHFTKNWGLRWYGN
                     AKDLLTNAPRDKVDVYANGDSAHPPKPGDMLVWTNSTYGHVALITRVGGGTLDIIEQN
                     VTGSGSATLSYNGSSVGSRWGSWVPAGWAHAKANGGGGGISWDCTKSAYNGGQYWTCS
                     GGSLYKCTSGVPQQRICPAGCNVMPLGTDDTCK"
     gene            23023..24057
                     /locus_tag="PKL17_05195"
     CDS             23023..24057
                     /locus_tag="PKL17_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44154.1"
                     /translation="MKQRRSVLGLAALAVAVSFSSSLVHAQSETWAAALRPGSGDKLT
                     LTWRQGLSSGDFTQLQVANGTAQAQRCTPDCTPIGKPTPLSEGQKEQLLSGLRSVGLE
                     SLRSADEAEMTADRELGLALPGKKARTIALPKNDWPTSSDGQGIATTLDDLLRTLVAK
                     AEARPQVKLPQNADELASLRISLTVTTNKQPGGQLQLEHGTLTIAPEEGSLPRMPRPR
                     PQSRLLSDAEKTQLVQALQGLDLDRLEASIPQRARPAVGDDDGRLLTLHLLPASALPD
                     PIAKVAKRGGAVSKTAAVPAAAQPTKQPRGLRRYVADWVRSPAGPAVRLLASWLAIHL
                     PTAPSGSAEK"
     gene            24181..25737
                     /locus_tag="PKL17_05200"
     CDS             24181..25737
                     /locus_tag="PKL17_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNK44155.1"
                     /translation="MAELRLSLTRRLSALTLVSACSAIAGCGAIGAPALPFKPEEATV
                     QNVRSAVKSGFISCRELASRYQLLHDSLDPSLHAVVTWNIDLLQDADRLDAVPTAQRG
                     NFHCVPLVVKDNIHVAGMQSTGGVLALSGGGVSTDAEVVLRLRTAGALILGKTNMPDF
                     ALDGTGTQSSYGGQTLNPYNRALTVYGSSGGTAAALGASLGIVGLGTDTYGSLIQPAS
                     ANGLVALRPTQGLIPNAGVMPLMNLQDTPGPMARTVEDVAAILELLVDKSQVGKGSQS
                     YTSQLKSTGLQGLSLGFDPAALQPLAMPMLTPSSEVSDLFYKALADISTAGGKTRQVT
                     VLTTLFASLQAATDGSFACMPVDFKESFGNYLASRSDLKTKTLADVIATGKYSDSAKG
                     FLLSAQAQTDTTQTSVACQKYLTARTAAQNAIVALMDKEGIDLLVYPAANQPAFPIGT
                     PPSGWFGFQMLSSPTGLPSLTVSMGTTPKNGAPMGLTLLARPYQEAKLVQAAYAYQQQ
                     RGPRPIPPKQ"
     gene            complement(25761..26387)
                     /locus_tag="PKL17_05205"
     CDS             complement(25761..26387)
                     /locus_tag="PKL17_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44156.1"
                     /translation="MRAFHVAAAAAVSVSVLLTGCGEERRQATDVEKQGVLAVLSTGL
                     HLRDAKQDSRLELPPGTETLINLVIDALCKASLTTMPVGDHVRETLTLNCKDGDFRGT
                     YIREQDHYTFDLYAKTSLDVTYKGDVTANPSLVDGWMELKGLKDLWLTDVSVSDRIDF
                     MGIQRDAQGCLVGGGMKVELHGHIPGKTVDVSTDALFGPNCGDVWVPK"
     gene            complement(26530..27612)
                     /locus_tag="PKL17_05210"
     CDS             complement(26530..27612)
                     /locus_tag="PKL17_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK44157.1"
                     /translation="MKIAIVGAGPGGSTCALQLARHGRHEVTLLDRDEFPRPKTCGSG
                     LGPRCLEVLKDIDMADHFQPLAQKISGLRFVGPRGNEAEMNANQEAAWIIPRSTFDAE
                     IAFAAERAGVSFRQNFHAKKMVRDNAGRVVGISDGKDTIETDLLILADGAHSRFSVDK
                     RPRRQIAAIMAWYEGVPYKKGILEMYFDQRVKPWYGWLFPETDTRVNVGICYDPDDPA
                     DPKRLLDEVIATHVGERLRNATQVGKYKGHPIVYAEGVGPITSPGAIWIGEAARLTNA
                     ATGEGISYAMRSACVAADVIARHERPDTSFLREYQAATAKKFALPLKTAVGFMNFVNT
                     PAFGLASSLITSRAVKSTMRYLLANA"
     gene            27750..28496
                     /locus_tag="PKL17_05215"
     CDS             27750..28496
                     /locus_tag="PKL17_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNK44158.1"
                     /translation="MTSRHPVEPDTTKGIGFWHGLRTVPRAVDVLRNVPQVLRLLLIP
                     LLLTALLDGLALYYGFGYLHQLILLHVPQSGLFGLLSGFLTLLAGVLVVAALAWTFGF
                     VYLTLCELVVDSVSEQIEEHLTGHSGSTDDWGSKLRGVLIGLGQSLLLTLIGILSALI
                     GMIPIVGPLIAIPLGATALGYGFFAISAGRKGHSLSQRWQKGKDHLAALCGLGLPLFV
                     INLIPVVNLLALPVFIVAGTLLYLDTEQRS"
     gene            28661..31123
                     /locus_tag="PKL17_05220"
     CDS             28661..31123
                     /locus_tag="PKL17_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44159.1"
                     /translation="MTRSAPVGSRLPVIRVENPLPALWIGFVIFILWTMLVLAMSSTP
                     PHAAANGVLPLRDPALEHGLENWSAWSDRLPIKAQTIWSQLLLGGSLLLGWVWLLTAG
                     LAFVVKPDGIAARAFSRWAALCSLLLITTYELHYSNRLSLAYQVGFALTQSALLEFVL
                     FFPQRVPILLRYPWLHVVARLSDVVLIILFLLAFLLEDKWIGVFRSSQEYTALVCSVI
                     SAAVMVWRIFHHTKRRRSQALVSATAVLPLHLLFVVAICADKLCGLQLLMIALPAGFG
                     GALGLIYAMVRNDLWDSELVVPGTGLRRVLIAILSFMGGLLALVLLRSLTMLSVQAQV
                     LLTLALISVSGPMHSFVGTWLVARLFPAEALYRPTVDELIVRFTDLRTRQAVIETVEH
                     TVLSVCACERAKLVSVSSPRRVSRNSEIHRRQTDSTQAPSDPGQSSNVPLLALPDAGG
                     ESGKTLFRLTQRAIRRALRGAGVDSLRPEQIDSLCNGNLVFVDSEHSMRRTSPGLWAW
                     LLVPVRFREQVIGVLVVSPKQRGQLFSSLDQELLRTIAHQAALALANAIALEELDELR
                     RAEHSVHRERLDRAISTIAAEIAHEIRFPINFFRMLVERQERALHQGKPLSEHDWAED
                     LDIGREEVARLERMADRLRKMALSRTVQPRPLSLRSITEHVRLLLADRLAAHTFKMEL
                     KEGIEVLADPDALIQVLLNLLANAIDASTAEGTIGLTSVADPDGKLRLVVWDDGPGFD
                     APVARLFEPWFTTKAQGTGLGLAITHRLVRAHGWEIVAERRGSRTCFDVVIPLGEWRF
                     CSTGEISLLESVSSDEKQKNAL"
     gene            complement(31133..32323)
                     /locus_tag="PKL17_05225"
     CDS             complement(31133..32323)
                     /locus_tag="PKL17_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK44160.1"
                     /translation="MRVKTATLPEPLSLESLLAHADWVRKLAYVLVRDEAKADDLTQE
                     TWLAALRSPPDPTRPLRPWLAEVVRNLSRMNARSTQRRNHREIASQREVTEKEQDTPE
                     RLLERAEAQRMLSELVLRLDEPYRATVLLRYFEGCSAEEIGKRQQVPAATVRWRLAKS
                     LEKLRSDLDVRHAGDRRAWQLALVPLGSSLPLTDPSVPVVTKGVPVMKTFLTLALVAS
                     VSGGALLIHKGSTGATKAAPSAVVAAPSAPATPSAVPSSARRDLPQRTQMLAQIQTIQ
                     KKTETSPSHETAPALNREYIRDQMRALIPMIKDCYEDGIRIDPDLHGKLFVQFTIVGE
                     PDVGGLVSESKIVEEGSTLKLTSVRECIRDVMYSAVFPAPPSGGEQVVTYPFELLSAP
                     PASE"
     gene            complement(32401..33168)
                     /locus_tag="PKL17_05230"
     CDS             complement(32401..33168)
                     /locus_tag="PKL17_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNK44161.1"
                     /translation="MSQLIRLERHGNTAVLFLNRPPAHALNLDLLLELRALIPTLRVP
                     DFRALVITGQGRFFSAGLDLFEVFAYPEDKAALFPTVFDEVFSGLFSIDLPVVAALNG
                     HAVAGGAVLAATADYRICTDAESQLSLSEIRVGVPFPVTALEIVRSAWSGPCFADLVY
                     RGRAFRPAEALACHLVDEVVPAAQLLERALALAEELGSRVPLAFATTKRALRKEGRDR
                     IAAVGGPGLDPVWTLWRSEEILTAVEVYRKSLENKRR"
     gene            complement(33191..>33448)
                     /locus_tag="PKL17_05235"
     CDS             complement(33191..>33448)
                     /locus_tag="PKL17_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44162.1"
                     /translation="NGLIAHFDGSAWAVIPTSVTDTISGIAGTSASDVWAVTVQGSVL
                     RYDGQQFKTVSRDELPSLQAISAVDGQVYIAGALGTILHKS"
BASE COUNT         6792 a        10355 c         9820 g         6481 t
ORIGIN      
        1 gtgctcggca agggcaccta caagaccgac gcgatgggca aaaagatcat cgacgatccg
       61 ctgatgcacg tcgtgataaa ggacatggcg ctcgacttct atgtctacat cgacgaacgc
      121 tatgtgcgct tcatgcgtca gacggtggat ctggatctgc cgctcggact ggatgtcgat
      181 gcgatgaacc agattgtgcc gatcctcggc gacctggcga tggccgtggg caacgtccgc
      241 gtcagcgaca gctcgcttct aaaagaatcg cccgcccagc tggcccgcct gttcccgagc
      301 ctcttgccgg tgctcgccgg ttcactcagc ggctcgctca gccccatcaa gctgccggac
      361 atcatgggtc tacagctttc gccggtccag atcacgtcga cggcggaccc gacgggcaag
      421 ctgtcgctgc tcgggatgtt cctgtcgatc aaactttcga cgatgccaat tgcgggagaa
      481 gacatgagcc tggctgccca agctgctgac gaaccgaccg agacgtttgc aaaacttctg
      541 agcgtggaag tcccgacggc tgccgagctg gccgttaccg cgaccgagca gaagtcgacc
      601 cgcgtcaccc tggatctcga tgccaagagc cacctaagtg gcgaccgtga atggcagtac
      661 cgcatcgacg gtggtctgtg gcgcccattt gatggccagc agcgcttggt catcgaagag
      721 cccgagctgc gactcgccgg cccacacaac attgaagtcc gcgcccgcgt cgccggtcag
      781 ccgggaacgc tggacccgac gccggccagg gtcgattttg tcgtttcgcc cgtggctgcc
      841 cccgcgtctg ccaagcccac ctgcgtcggt agcaagtcgg attcgtcgtc tgaacaatcg
      901 tcaggtcagt cgatgacggg ttgctcgacg agccgcagcg acacaagccg tggcacattg
      961 atcgcatttg cgatcgcgct ctgcggtctt ctgtcgcgaa ggcggctggc gaaagtcggc
     1021 acgctggcca tcgcggcgct gctcggcgta agccagaccg cctgcaacaa caacccgacg
     1081 ggcactggcg acggcaccga caaaccgccc gtcgaagtga aaaaacccaa gcccgaatat
     1141 gccgcaaacg acgaggtcgg tcgctaccag agcatcgccg tgaccgcgga caaaatttac
     1201 gtcagcgcgt acaactcgac gtttggcgac ttggcgttta ccgaaatgtc gatgccgagc
     1261 aaccagagca cctggctgcc ggtcgacggt ctgccgacgg gcgatccgga caccatggac
     1321 ccgtcggcgt atcgatcggg ttatgccgat gccgccgatg atgtgggtcg atttacttcg
     1381 ctcaagctaa accaaggcgg aaaccccgtc gtcgcttacc aagacgtcac caacagtgcc
     1441 gtcaaaatag ccgcccgcaa agacaacaag tggagcacgc tggtcgtcgc cgatcagaac
     1501 gaaggtcgtg gcgttggtgg ttttgcccag ctgatcctcg acgccagcga cgttccgacc
     1561 gtggcgtata tggtcgccgg aaacaaaaaa tccgacggca aagtgttcgc gcagatggtc
     1621 gttgcgacgg ccatgagcgc aagcccgcag agcccgtcgg acttccaaaa gaaggtcgtc
     1681 gaagaagtgc aggtttcgtg cgccggtctg tgcggcacgg gtgaagcgtg cgtgtacgcc
     1741 gatcccgcgg tcaaagatcg ccaaaagacg atctgcaaaa agaccgaatc gacctgcacg
     1801 ccaagctgca agagcgggct cgcctgcgtc gctgccaagt gtgtcgatgc gctcggtacg
     1861 ccaccgagcg acctgccgca aggcaccggt ctgtttgcgc gactcgtcca gaaccaagac
     1921 agccgcttcc ttgtgttcta taaccgtgcg accggcgcgc tgaagatggc gacgggcacc
     1981 gactggaaag tcacgacgct cgatggcggc gacggaaaag tcgacaccgg acgccatgtt
     2041 ggtgcctttg cggccagcga tggaaccctg cacgttgcct acagcgatgc cgacggacgg
     2101 ctgttttaca aaaacgccaa aggcacgatg atcggcaaag cggagctcgt tgacgatggc
     2161 aaccgcacga tgccaacgcc cgacgtacac agcgttggcg ctggcgcgac ggtggttttg
     2221 ggcgacagcg gcccggtgat tgcgtaccaa gacgcgaccg cgggcacgct ggaagtggcg
     2281 caaaaagcga tgtcgtggac ccacaagacc gtcggtgccg agcccggcct aagccgtggc
     2341 tactatccgc agctgttcaa gtggaacagc aagtggtggg tttcggacgt ggtctatgac
     2401 cgcaacgccg acgcgctgac caccctgcgc tttagcgaac tgagccaatt gaccccgtag
     2461 ttacttctct tgcgaaaacg gcgcggtata gttcgcgcca tgtcgaccat ctcgggcatg
     2521 tccggcacgt cctcggccat gccccggccc caagcccccc cgccaccgcc gcaacaaaac
     2581 aagttccgtc gcgatccgcg gctggctgcg gtctacgatc gagacatttc accggtgtgg
     2641 acccagccgt ttggcaagct cttgcttggc caggtggcca cacttcccgg caagaccacg
     2701 atcctcgacg tgatgtgcca caccggagat cctgggctcg aacttctgcg ccgttttccg
     2761 gattcccgtt tggtcgcgat cgatccgtcc gcggccatga ttgatgtcgc gcgacagaaa
     2821 gccggaccgc tcctgagccg ccgggtcttt ctgcgcaccg aagcgggcga acccaagctg
     2881 ccgttcgacg aagcggcgtt cgatctggtg gtgtcaaatc tggggcttca tgaatcggcg
     2941 cagccgcatc ttctgctgcg tgagctggcg cgcgtcgcca agccaggggc gcagatcatc
     3001 acgacactgc cgctgcgcgg aacgtttgcc gaattttatt cgctgctcga aaccgagctg
     3061 caaggacgcg agctggaaca gcagcggctg tcttcccacc tgcaaagctg gcccgatgtc
     3121 gacgtggtgc ggggctgggc caaagccgca ggtctggccg aagtcgagct tctgatctcg
     3181 ccgttttcgc ttctgtttgc cggtgcggtc gatctgtact acgcgccgct tatcgaattt
     3241 gggccgctgc tcgcgtggaa gtcgctgttt gatcgcgatc ctgctgggat gcaagcggtg
     3301 tttgtctcgc tgcgcgatcg gatggaacag ctctgtaccc agcacgaaac gccgccgctg
     3361 ggtctgcgtc cgccgctgcg caagccactt gcactgaccg tgcgagcggc ctgtctgcgc
     3421 gcacgccgtc cgctgtagga atctttcagc caaacgtcaa tatgagtcat tttacatgcc
     3481 tcttattggc gcgtgccgcg gcatcggaca cggcgtagct ttatgtcttt ttgcgaaacg
     3541 gttccgtcgc ccgcagccac tctttgctgc gcgattctgg atcggcgtgg gtcaggacat
     3601 cggtcacgac acaggcacta tctgccccgg cttgcagcac caggggagcc cgatcgggcg
     3661 taagaccgcc aattgccacg agcggaatcg cctgaatctg ttttttccac tgtccgattc
     3721 gcgcaagtcc ctgcggcgga tacttcatca ctttcagcag cgtcggccag atcggtccaa
     3781 gcgcgacata atccggacac gccctaagag cggtctccag ttcttccggc gtgtgcgtag
     3841 acaccccgat ccgcacgttc gcccgtcgca gcgctgccag atcggcttcc gccagatcct
     3901 cttgtcccag atgcacaaag tcgcagccca gatcgatcgc ggtctgccaa taatcattca
     3961 ggatgagcgt cgcgccatgc tgcgcacaca gcgccatcgc ttgctgagtt tgccggatga
     4021 tctcgtcggt gggctggttt ttgatgcgaa gctgaacggt ctttacgccc aacggcagaa
     4081 gccgcagaag ccacgcaaag gaatccacga tgagataaaa aggatcaagc acgctttgac
     4141 tccgaaaagg ccagaccgag gacaggtgtc gacggtacgg ccatgtctcg cggttccatt
     4201 gcaccggcca tatacgccag tctgccggct tcgatggcca agcggaatcc tttggccatc
     4261 aggaccggat tgccggcttt ggcaaccgcg gtgttcagca aaattgcatc gtaaccaagc
     4321 tccatcgctt cggcggcgtg cgacggaacc ccaagccccg catcgacaac cagcggcacg
     4381 cccggaaagt gcgctcgcat cgctttgagc gcaaaccgat tgttaagtcc gcgcccactg
     4441 ccaatcggcg cgccccacgg catcagcact tcacaaccta tcgagagcag cttttccgca
     4501 acgaccaaat cgtcggtggt gtacggaaac accttgaatc cttgcgccgt cagttcgcgc
     4561 gccgcgtcac acagtccaaa cacatccggc tggagcgtgt cggcgtgtcc tatcacttcg
     4621 agcttgatcc agtccgtttc aaacacatcg cgcgccatct ctgccgtctt gactgcttcc
     4681 gaaaccgagt gacagcccgc ggtgttggga agcactttga cgcccagttc gcgaatcatc
     4741 gaccagaacg cacgaccacc gctgctgctg ccactggact ctcggcgcag cgacaccgtg
     4801 acgatctgcg cgccggatgc cgcaatcgcc gcagacaaaa tcgctggcga cggatattgc
     4861 gcggtgccaa ggagcagtcg acacgcaaac gactcaccgt aaaagcggac attgtcttgc
     4921 acgctcagcc tccctgcatg ggaaccagga cttcgaccac atccccgtct gatatcgcgg
     4981 tcgcgtcgcg atcttttttg cgcaccaccg tgccattttt cgccgtcgcc accggcatgt
     5041 cggtatagcc cagctccgtc accaaatcgg ccagcgtctg cgcggcgacc tcacgcggct
     5101 cgccgtttaa cacaagcttc atcgctcgac ctcgggaaaa gatgcgccgt cgaaaatgat
     5161 cgctgcagcc cgtctaccga gcgcgggcgc gaccagatag ccgtgacgat acaggccatt
     5221 tatccgcaag atgtttcctt cgcgccggat gcgcggcagg ttgtcgggaa acgccggacg
     5281 gacatcggtg ccgatttcga caatctcggc ttccccaaac gctggatgca gcgcatacgt
     5341 cgcattcagc agctcgatca tcgagcgggc agaaatccga cggcgatcac tgctttcaat
     5401 catcgtcgca ccgaccatga agtgatggtc cgcgcgcggc accacataga ccggaatccg
     5461 tgggtgcaaa agtcgaatcg gtcgcgacaa caccagctcg tcgctgcgca gaatcagcat
     5521 ctcgcctttg acgccgcgca gatccggcag cacatcccgc gcttcgtagc cgcgacaatc
     5581 gagcacccaa tcggcagtga tctgctcggg gacgacttcg gtttcagata caagctgcac
     5641 cccaaacgca cccagcagcc gcgttttgag ccacgtcagc gtctttcgcg ggtccaggtg
     5701 cgcttcgtca cgaaaataga gcgccgtttc aaaccgtccc gtgagcgctg gttccagcgt
     5761 ggcaatcgcg tcgcgcccga tcgtctgatg atgggaagtc cgcgccgcaa aacgccgcag
     5821 atccggcaga tcgcgcgccg gcgccaccac cagcgtcccg tgttcggcca cgtcgggaaa
     5881 ctgtgtccgc caatacgtaa gcgactcttg ccccagttcc ccaatcagag gctcggcgct
     5941 ttccagttca caaaacagcg ccagcatgcc gccggcaaag tgcgaacagc caagccccac
     6001 gtcgcccagg cgttcataaa gccgcacgtt ggcaccgcgc atcgcgagct gatgcgccgc
     6061 agtaaggccc atcacgcccg ctccgacaat cgcgacatcc actttgtcca cactcccctg
     6121 tctggcacga cgttgcagcg tcagcacagg agccgtgcca aagaccccca cggtctaccg
     6181 cgaggtcagc cgatctgtca atcggcccgt cggatatcgc gacgcacagc gactacgagt
     6241 tgcccggcgc caccggctga cggagcagcc tttgcacgct accgcggaac tcccagtgcc
     6301 agttttcgct cggcaccgtg cgctgaaaac cgaactcgtg cgcatgacgc gacatccaca
     6361 gccgcacccg tggcagccgc atgtcgatgt cgagcgcatg tccgctctgg tgattcgaaa
     6421 aaccaggccg cgccgccaag ggtccacgcc cgcgacgata caggtcgaac acgtcctgct
     6481 gctgctggtt tgtgcgaaag ccgctgttga gaaaaagcga cagcccaaac cgctgcgccg
     6541 ccgtctgcat ccggtaaaac gccagcgcgg tatgcacttc ggcgcgcacc tgcgtctgcg
     6601 catagggact cggcatgtcg acgatcaaga gcggctgcgg ctgaccattt gcaaagccaa
     6661 gcacaggttc ggtttgggcc gcccgctggg tttcgagcag tgccccgacc accacggcag
     6721 acagccacga tcggtcttcc ccggcctgcg ccgacagctt gagcggagcc cccgaaagcg
     6781 ccgccgcaat gagcagtgaa ctgccaaaca ttgcagtcat catagaacta cttacagaag
     6841 tgcgaaagct gcaaattcgc gaaattctcc actttttgca gcagacgatt tcactttccg
     6901 cgcggcatgg cgtgcgcggc gcaacttggg tgtaagctgc cggcacatgg ggcgtaactt
     6961 cccaaacacc gacagacgag ccgaagcggg ccgcgtcgca tttggcccca cccgatgggc
     7021 cagccgggtg gggggtctgc tcgcgctcgg gctgtcggcg tgcatcgaac cgacctctgc
     7081 gcccccggaa aaaacgcccg tcgaaagcgc ccgtcccatc cccggcctga ccgcgacgcc
     7141 gcatgaacag atcgtggccg ggcacctgct ggtggagttc cgccgcggcg ccacaccgcc
     7201 gattgtgcag ccgccctcgc ctgcgacgtt gttgccaccg gatgaaaatc cagcgacggt
     7261 ggtgcttgcg gacgaacgac tgattcttga acgtgagctg tggccgccac cggcaagcga
     7321 ttccccgctt ggacagcgcg atccgcaggc cgggcagatt gtgctgctcc ggcgggatgg
     7381 aatcgacgaa tcggcgacgc tggcgctgtt ggacaaagtg gcgagcgatc cgcaggtccg
     7441 ccgcgtcgag cccgatcgac tgcgtcacgc cacttcggtg ccaaacgata cgctgtggtc
     7501 gtcgcagtgg gccttgccgc tgattcgcat gccacgggcg tgggacatca gcaccggatc
     7561 gcccgacgtg gtggtggcga ttctcgacac tgggatcatt cacgatcatc ccgacctcga
     7621 atcgaggctg attcccggtt acgacttcat ttcgcgtcca gcgagtgccg acgatggcga
     7681 ccgcgtgcgc gatagcgacc ccaccgacac cggcaccgtc gataccagcc gtctgcatgg
     7741 cagccacgtc gccggcatca ttggcgccgt cagcggcaac gcgatgggaa tcgccggcgt
     7801 cgatcagaag tgtcgacttt tgcctgtccg ggtgttgggc gtcaaaaacg gcgacggcat
     7861 cgacagcgac atcaccgacg cgctgcgctg gctgaccggc gcccagcttg gcaccatccc
     7921 ggtcgtttcg cgcaaggtcg acgtcgtcaa catgagcttt ggcggccccg gtctttcctt
     7981 tacgctccag cgcgccgtcg atgaagtgat atccgcgggc ttgatcgtcg tcgtcgctgc
     8041 cggcaacggc ggaaccgaca ccaccacgta ttcccccggc gggctcgata acgtgatcag
     8101 cgtcggtgca tcggatgcca aaggcaagcg cgcggtctat tccaattacg gtccgcgcgt
     8161 cgatctgctc gcccctggtg gtggtctgtc ggaatgggac tacgaaaccg acttggccgg
     8221 actgcctccc gacggaattt tgtcgaccta ccgcgacgaa ggcgtcgccg aaccgaaacg
     8281 tccaccgttt acctattcac cgctcactgg cacatcacaa gcggccccac acgttgctgg
     8341 catcgcagca cttgcgcgat cgcttgtgcc caccgtccgt cagcgcacga tgggcctgct
     8401 tctgcgcacc gccgccaacg ccaaatatcg ctgcgcaagc gatgatctca gcggctgcgg
     8461 cgccggactt gtcgacgcgg aaagcacgct gctgctcacc caattgcaaa agcgctgcgg
     8521 ctgcgaaggc gacttgtact gcgtcgatgg aacctgtcag acgccgccgc aaatccacga
     8581 ctcgatctgg gaccgtccgc tcgtgcgggg tgggtactgc cagctcggcc tgtcgagcga
     8641 gccagcggca gggccgtggc ttgggctttt taccgcggcg cttgtgctgc tgctgctttt
     8701 tgcgctaaaa gccagaccga aagcatgaac accgacgatg tggtggtaac tgggcttggg
     8761 gttgtgtcgg gtcttggcaa agacctgtct gctttttgcg acggcctgtt ttcgggtcga
     8821 tcgggaattg gaccgctgtc gctgttttcg tgtcccgacg cacgcagcca gcttgtggcg
     8881 caggcgccgg acccggatct gtcgcgcatt ttgctaccgg gctgtacctc gcgtccggat
     8941 cgctttggca tcgcggcggc gctggcagcc gtcgaacatg ccggcctttc gacgcccgat
     9001 ctgcacgacg cagcggtcct gtttggcacc ggcacgggcg gcgcaatcga aaccgaacag
     9061 tatctgcgag agctgcaaga tgggatgttg ccatcgccca aactgctggt cacgcatcag
     9121 cccgcgagtg tgaccgatct ggtggcgcga attctttgtg catatggtcc gcgcacgacg
     9181 gtgatgacgg catgtagttc gtcggcgatt gcgattgcgc aagcgctcga ttggattcgg
     9241 ctcggacgct gcgaggtcgc gctggccggt ggggccgaag cgctgtgcaa gctgacggtg
     9301 gcgggctttg gtgcgctgcg ggcgacgtcg ccggaaccgt gtcagccgtt tgataaaaac
     9361 cgcaaagggc tgaacctggg ggaaggcgcg gcggtgctgg tgctggaatc gcgtcgccat
     9421 gccgagcggc ggggcgcgaa agtgctggcg cggcttttgg gggcagggat gagctgtgat
     9481 gcgcaccaca tgacggcgcc gcagcccgaa ggaatcgggg cccgccaggc gatggaggca
     9541 gcgctctgcg acgccaaagt cggtcgcgaa gcgatcggct acatcaatgc gcatgggacg
     9601 ggaacgcctc acaatgacgc cgcagaaagt cgggcggttc gggatctttt gggagatcgc
     9661 gcgccccacg tcccgatgtc gtcgatcaag tcgatgtgcg gacatacgct tggggccgcg
     9721 ggagccatcg aagccgcagc ttcggtgcta tcgcttgtgc acggcgtgct gccaccgacg
     9781 aaaaacctgc gcgatcccga aaccgaattt gggctggatt acattcccga agtgccgcgc
     9841 caaaaacagg tcgatgtcgt gctgtcatcg agctttgcat ttggcggcaa caacgcagcg
     9901 ctgcttttcg gtcgggccta ggcgcacggc gagtggcagc caggaaaagt ccgctacact
     9961 ggctgtcgtg aaggaaaccg tccgcacgtc cttgggcgaa atctaccgcg agccagactc
    10021 tccgctggtc gtgctgcggc gcagttcgac gcctgcgcta gatgaagacg agctgcttgc
    10081 catgctggat cgcttcgatg cgattttgcc cccgtcgcgc cgtcgcacgc tggtgttcct
    10141 gatggacatg cgggacgggc cgatgcgcaa cgacgaagaa tatgaacagc ggatgaacca
    10201 cgcgatgcag cgtctgtgcg ccggctttgc gcgggtcgca gtgctgatgc gctcgtcggt
    10261 gggaatcctg caagccagtc ggctccgtcg cgaacatccg atcgtggccg gcacagttcg
    10321 cccgtttttg gatgaaacgc aagcgcggtc ctacctcctc acttcttccg aaaaaccgag
    10381 tccgtaattt caattacaaa acacgcgcct agacaagtgc gttcccgtcg agctacgcta
    10441 ggtggtaagc ccttgtcgaa gctagccgag atcgtccaac gcctgcgcaa gtgggtgtcc
    10501 aaaaaaggcc ccgcgcagcc ggtcgtgccc gatgaaagtc ctgtgttgcg acaaaagcgc
    10561 gagccgcttt tccgctgcgc gatgcagatt ctcgacgatg ccgccggggc tgagcaggtc
    10621 ataaggcaag cacttcgcga agcggagcgc ggagtgatgg cgccaaatgg cccagcgcca
    10681 cccaaacacg acgagtggct tttgcggcaa gtggtcagtc tgtcggtgca gcggctcaaa
    10741 gcgctgtcta tcgtgccggt ggttccgccc ctgcccgacg cggcccgcgg tggcagtgaa
    10801 agcgaaagcg acggcgcaga tcccagcgcc acgcaaggcc gcagcacaag tgaacagcag
    10861 cgccgcgagc gattgagccg cgcgctgctc aagctgccgg tcgagatgcg ggtcacgctg
    10921 atccttgcgc ggatgcaaaa acgaccgctg acggaagttg cgacgctgct tgggacgacg
    10981 gaaacgacct gtctgttttg gctcagccac ggtcgcaagc agcttcgccg tgcgcttcag
    11041 cgcgacttgg cgcccggtcc agaaaacggc ggaccgctgc gcattcttgt ttcaccggag
    11101 actccccatg acgtgcgcgg aaacaagaag gcaattgccc gagcctgagc cagttcacgt
    11161 cgccagcgtc agcgagcacc tggcaagctg tcccccctgt cgcgtcgagc aagaagcgct
    11221 gcgcgaagtg gacagacgac tagaacagct cggtgaacat cgtctggcag ccgccgcgca
    11281 ggtgctggcc aaaacccgtg gtccgtcgcc acataccccg atcttgccga cgcagccaac
    11341 gatcgcggtg tggaaaatgg tcggcgcggc ggtgctcggt gtactgattt tgtttggcac
    11401 gttgctgctc tttttccgcg gcaaataact tcgcgcccgc gcgcaaaaac cccacacacg
    11461 gaaaccgcca tgagcaaaga cccgaccgag tctgcggaca agcacgatca cgactggatt
    11521 gacattggat ctgccgacga gctggccaag acgccgctgc aacagatcat gctcggcagc
    11581 acgcgcatcg ccttaagtta cgcgggcgga gtctttggtg cggtaagcgg tgtctgcaat
    11641 cacgccggcg gcccgcttgg gaaggggact ctcgacggag attacgtagt ctgtccgtgg
    11701 catcagtgga agtttcacag ggtcgaaggc caaggcgagc gcggatttga agaagatgtc
    11761 gtaccggcgt atcacgtccg ggttcgcgac ggtcgcgtcg aggtttcgcc caaaccacac
    11821 aaaaaacggg ccaaaaaacc gcacgcaccg catccgctgt cccgcacacc ggaaagacag
    11881 ccgggacgga ttcgcgtggt gggaatttcc acgacggcga tgcagaaaga ctttccgcgt
    11941 tattcgacat cggatgcgct gctggaagtc gcgatggatc acgccaaaca agcgggctac
    12001 gacacccaga tgatctatct gcgcgacctg tcgttccgtc actgtgaagg cttttattcg
    12061 aaagcagcgc gggcctgtac gtggccgtgt tcgatcacgc agatggaccc aaatgaccag
    12121 ctcgatcggg tctatgaagc gctggttcac tttggcgatg tgtttctgat cgcaaccccg
    12181 attcgctggg gcgcaccgtc gagcctgtac ttcaaaatgg tggaacggat gaactgcatc
    12241 caaaaccaga tcacgatttc aggaaaggcg cttctgcaaa acaaagtggt cggcgcgatc
    12301 atcaccggcg gacaggacaa cgtgcaggcg gtggcgggtc agctgctcgg attttttggc
    12361 gagctgggtt gtcagtttcc acagttccca tacatcgcgc actcacgcgg atggactgcg
    12421 gaagacatgg aacgcaacat cgactatgta aagcaaagtg aggagctacg gcaggccgcg
    12481 cgggagcttg tggaccgcag tgcaaacctg gcagagcggc tgattgcaac gacgcctccg
    12541 cccgtgccac gcggcggtcg caaaggtcgt tcgctgccgc ttgtgaaaga gtagttatcc
    12601 gcccgctgct ttgagtccgt ctctggccag ctcgttgcgc ggcgagatat ccatgacggt
    12661 tcggaattcc gccgcagcct cggcgtattt gcccaacttg agcagcgccc agccaagtcc
    12721 caccttcact tcgacatcgc ccggatacag atccgcaagc cgtttgtaaa acgccgcaga
    12781 ttccgcataa cgctgcaggt tgtagcaagc gaacgccagt cgcagcgtcg ccaaatagtt
    12841 ggccggatca agctttagga tcgcccgcga ctgcgtttcg atgtctcccc agcgacgcag
    12901 cgccatctgg ggcagcagaa ttcccagccg cggctcgacc gcagccggag cctgtgcaat
    12961 cgcgcgtcca tacacctcga ccgcttctgc cgagcggccc agccgataca aaagccacgc
    13021 tttgcgcagc gtcaccacat agccatcgcg tcgcggtgac gacatctgat cgagcgcagt
    13081 cagcgcatct tgcagcttgc ccaccgcttc actgtcatac gatcgctgga tgagatccgc
    13141 atttccgtcg gctgaagcga cagaaagcga cagcgcaagc acgccacaca ccacaagcaa
    13201 ctgcttcttc atcgcgtttc ctctttggta gtaggcggag cggtgattcg ctcggcgcga
    13261 accgtccgtc cccgattggt cacttcgatt cgtaccacac ccacgccgcg ggcaagaagc
    13321 acccgcgtcc gcagatccgg ttcggtgtct gttccaccgc gctttttccc atcttcggaa
    13381 gcacccgcat caccgtccac aagcgactgt ccatcgactt gcagaagctg cccatcccgt
    13441 cgggcacata gctcgacttc gattccgtcc cgcgccctta gcggtgcgac aaagtcatgc
    13501 agaagctgaa gtcccagcgg gcgcaccaaa cgacccggaa gcagatcacg gaagcgcagg
    13561 gtttcggaac tttccagcgg aatccgcggc agtgcagtgc gcagaagata cagcactgac
    13621 cgtcgagaac cgctcacatc ataggtcgta aacagatcat ctgtcagcac gtagcgcagc
    13681 accgttccat acgtcagcga gcgcagaagc ggctgtccaa acagatccac ttccagcaaa
    13741 attcgctctt cgcgatcgcc gatttggaac cgccattccc cgccgagtcc gggtgccagc
    13801 gtcgaaaaaa acgatcctcg atcttcatct ggttccagat tccggacctg ctgtttttcg
    13861 accggaatca gcgcagagtg cagaagctgc cggtccgcat cgatcaccac agcatcggca
    13921 aacgaacacg gaatcgtcgc agcaccgatg tctggactcg cttgcaaatg aaagtgcaga
    13981 tgcggttgcg gcgatcgtcc agagcttccg cacagaccga tcacatcacc ggcttgcacc
    14041 acttggccaa gctggacttt gaccgatccg cgcgaaagat gcgcaagcag cgaatagatt
    14101 ccgaccccat gatgaatcag gaccaggttg ccccagttct gtttcagatt catcgtccca
    14161 accaagttgt ccggaatgtc gttttcgatt cgcaccaccg ttcccggcgc tggcgacaaa
    14221 acaggcaaac gaaagcagta atattgatcc ggtttggaac cgtcaccccg gaaggctttt
    14281 ccgtccggtc ccagcacttc aaaatcccag gcatgacgcc agcgatcctt gtgtgtgtgg
    14341 gcaccgtcca cgccttgcgt acagatccag gcgccgcgca gtggcagtcg cattttgaca
    14401 ggctgagacc aaccaaagcg caaaagccgc gcgcgcagat attccaggtt gtcttccggc
    14461 gtcgctagca tcacatcgac cgctttggga tgggaatcgg aaacgcgctg ccggagcgcg
    14521 agcagaatca tcaagatggt gacgttaaac ggaaccacca tgatgggtac gccaagccgg
    14581 ttgtgaatcg gcaaaagtcc agcgcacaaa agcgttgcga ccagcgaacc aaacagaccc
    14641 agaagccaag cggaagccga aggaacaaac cacacaccgc ccaacgcaac ggccacaaac
    14701 gcggcgttat agcttacgac aaggacgatg gtttcgacgg gaagtcccaa aaaatagatg
    14761 ccgaacagat acgccaccgc aaaccccatc gccgcgagca tcgcagagat ccgcgagtgc
    14821 accaaaatcg caaacagcac gatggcgcca acctctgcac ggggcaaaaa gaacagcgcg
    14881 ccaaggccac gcagaagtgc cggtccgtcg ctgtcgacga gttctgtcac aggcggcgct
    14941 ttcagtccgg caggttccac caccgccaac accaagtggt agacgatgat aaacggaatc
    15001 gacagcatcg gcagatgcag tctgccgatc gatgcacgca gtccggctgc gaccaccgta
    15061 cagagcgcgc cacagaagac ggcgagcgcc agcgaaccgg gtccaaccac acaggtgtga
    15121 ccgattccca gaccgcacaa aagcgcgtta tagccgtagc cgccgctctt ccgttcctcg
    15181 ggttcaagct ggaagagttt ggcgcaaagg agtgcggacc ccgcgcaaag gagtccgtac
    15241 cccgccgcaa tcggcatcgc gagcgttgcg acaagcaaaa gcaggcccac ccacaccgat
    15301 cctgaaaaca gaatctgtgc gtagcttaaa agcagcgagc gtataaatcc ccaaagtcgc
    15361 aagcgttcgt cctttgccta tcagtgcggc gcttcgtttt tttgcggtcg ccccacaccg
    15421 ttttgcatct acttgagcca ggccggaact tcttcgagtg cagccagatc gtcgagtcct
    15481 tcggcgcgac gaatcaagcc gacttgccca tccgtgctga ccatgacaac agcaggacgc
    15541 atggcgataa actgcatcca ctgggtcacg ttgtacgcgc cgacgttgcg gaagaccagc
    15601 cgctgacccg gtgacaccga ggggaactgc accgtgtcgc gcagcacgtc gatgttcatg
    15661 cacaaaggtc cgtacagcac ggtcggttcc aaaagaccgc caaattcctg cgcaggaacg
    15721 acatcgtgtt tgtaccaaaa cgcggtaaac agaatgttga cgcccgcgtc gagaaccagg
    15781 ccacgacgac catcgggaag acgcttggtc gcttccaccg tcgaaatcaa gtatccggaa
    15841 tcatcgacca gcgcgcgtcc ggtttccagc accagcgttg gcagttcctt ggacggatat
    15901 tcaagcaggc tcagaccgtc gcagattgca tcggcgtagc gctcaaacga aggactgacc
    15961 tgttcccccg gaagatactg cgccgccagt gtatttggcg atgcaaaccc accacccagg
    16021 tcgataaacg acagcttgat tccgtgcgcc gcgcggatct cattggcaaa gcgcgccagt
    16081 ttcgccgccg cttgtccata ggattcaggg gccaggataa acgttccgat gtggctgtgc
    16141 agcccggtca gttccagacg gtcgccggat accaaacgat tgacggcttc gcgcgcttgt
    16201 ccggattcca gattcacacc aaagcgactc cacgccggaa gtccgtcaat cgccaagttc
    16261 agacgcagcg cgaccttggg acggattccc attttttcgg caatccgttc gatgcgcgac
    16321 agctcgtcaa agttgtcgat gtgaatcatc gcaccgccgg acagcgcacg ctcaatcgcg
    16381 gcgtcaggct tgtacggacc gttccagtga atgtgatcag gaacgatgcc ggtgcgaatc
    16441 gctttttcat actcgaacgg agagacaact tccgcccacg atccttcttt gtggaacgtg
    16501 cgacagatgg cttccaggta gtttgtctta tacgaccagg caatccggac tttggcgtag
    16561 cgacgagaaa acgcctgctg aagctcgcga tagcgattga tcagcgtttt ttgggaaaac
    16621 acaaacagcg gcgatccgta cttggccacc agatcggaaa ccgcaacgcc gtcgatgtgt
    16681 gtgagtggcc gcattccttg caaacggcca aacttgttca tctgtcccat ctggtggcgc
    16741 accagcgtgg ggcgctcgta aggaagcttg ctcatcggga aacctctccg gtggtcacca
    16801 tgttttggaa gtcttcgatc cgcgcgatct gatcaatcga gatgcggaca aacagcgtgc
    16861 cgacttgata atccgacgcg atggatggtt tttttcccat cgccagctcg acacatgctt
    16921 gcggcagatt cattcctgct cccgcgctta ggtacaccca agccggaaag cgcggattga
    16981 tctcgatcaa gtgatattcc ccgtttttgt cgcgcatcag ctcgatttca cacgggccgc
    17041 gccacttggt cgccgccatg aatttacgcg gcacatccaa tagcgccgga tcgcgcaccg
    17101 tgattccggc ccagcctttg cctttgtcgg tcagcatcag cttcttcatt ccgacggcac
    17161 cgaccagtcc cccttcccca tcccccagtg ccacgacgtt gaattcctcg ccgcttatca
    17221 gggtctgcac gaccaccgga atgccccact gcgcgaccac tttgtaaaac gcatgcgtcg
    17281 cttcatccaa gctgtgcgca agggtcgcgc cgtaatacag accctttaca aacatcggat
    17341 agggaacgcg ctgatgaacg cgattcagtt cctcgatcgt gttcaccgtt gccgtcgctg
    17401 gaaccgaaat tccagacagc tgcccaagct gcggaagtct tcctttttcg cgcagcgtaa
    17461 actgctcgcg cgttggcaaa agcgtccgga ttcccaagtt cctcagcttc ggttccagat
    17521 ccaaaaacgc gggcagctct gcatcgagcg tcggaatgat cacatcgatt ccgactttgc
    17581 tgtggacata ctccagccgc gaccaaaatg ccgtcagtcc ttgcgaggga tacggcagca
    17641 taaacacttc atgcagaagc tcggtcgcgt agattccggg gtcgagcgca tcgtaagcca
    17701 gaccgacgat tcgcgccttg ggaaaagcgt cacgcagggc ccgaaccacc gccacacccg
    17761 gagccggatt gtccgtggca ttgagtccgg tgactgccac cgtaagaggt ttttcgctca
    17821 tcgcaccctc tacttttcag cgaccgtgaa gttctgcgga accactccgt gctggcgcag
    17881 aagttggaaa aactccgcga gatccgattc gacatcaccg tgaacatcga aacgctcacg
    17941 cagtcgcgcc acaatcgctg gacggctcag tccttcgcgc agcgcgtgca ggatcgcaag
    18001 tcccgccgcg ttggttgtga acgtcgcgcc ggtatatgga tcgaacacaa acccagtgtc
    18061 gctgagagcg aggtctttca gatgtgcacc actcataagg actcctagaa ggctttgctc
    18121 aggccgagtg tgaagtagtt cccgctgctg tctgtgctac gtgcaacgcc ggaaagcttg
    18181 cgcagccgat cgaagcgata gtcgacaagc agccgaaaat cacgaggcag acggatcgag
    18241 acgccggccc aagcggaacc ggcgacgcgc tcatacatgt tggtgatgac cgatagttca
    18301 agctgggcag gacggagctg ttcgccgtag ctgccaccgg cataggccgc gatgcgaccc
    18361 cggacatagg aaaattcgag aaagccgttt cccaaaagac ttcccgaagc ccactgtccg
    18421 acgccgccga gctgaaccga aaaccccttg gcaatcggca gccgccacga gggagccagg
    18481 cgcacgatcg tcagatcgga atacacgctg gtcgcaaact gaagctgcgc atcgccaagc
    18541 ccaagcggac ttatccgcag cccaagcccg gcatgatgcg acgcctgcga aagaccgcgc
    18601 gaatcgacaa gaaaggcata ccgcgcgatc aaagacagcc gcgttcccga atatcccgcg
    18661 tgcagatacg cttcgtgctg atcaaacgtc gtcgacgata tcgtcgtgac ggtcgttgtc
    18721 atcggcggca tcggcggcac gccaggccgt ggcggcattc ccggcgtcgt cacctgcgtc
    18781 tgagtctgcg tttgcaggaa aaactcgctg tagcgatatg cggcaccgat cagccacttg
    18841 ccgtcgatta ccagctccag ccgcggcgac acggcaatgc cgccttggcg ttcgtagtga
    18901 ccggcatacg ccatcccagt caaaaacgcc gacgtattga gcgcaaaacc cgccggttcc
    18961 ggacacgcga gcagcccttc tttggcccct gcatgattgg gattttgcgt cgccagcgtc
    19021 aggaaatgtg cccgcgcttc atcgcagcgt gaaagccgct ctagcgtcca gcccattccc
    19081 aagtgtccgt cgatgctcag cggcgaaatc ttcagcgccg tttcgtagtg tgaaaatgcc
    19141 cgccggtacg actcagcccg aaaccgcagc cacgccatcc gcaacgccag atcgagatcc
    19201 tgcggatagc tcggcgcgat cgcttccagt gcttccgccg ccgcatccag ccgcccttgc
    19261 gtttcaagct ccgtcgctac gcggcttgcg tcaaggtatg catccgcgaa ggcaagctgc
    19321 ggcgcgcccc caagaacgaa acccaaacac cacaaagctg ctcgccgcat catcgaaaaa
    19381 tgctcccgac cctggtcggc ttccacatcg cttgtttgac ccttgaatag agaaagtgcg
    19441 cagcgcggcg attgcacatc gcgcgcaaaa aaatcaccgc gcccacaaat tgtcgccgtt
    19501 tcccgtcgaa aactggccaa aaaagtcggc ccccggaaac cgcgcggaca gcaggcgaac
    19561 cgcagcgctc cgctgggtac ttggttcaac ctttttgttg ggtgcgcttg gtggcagcga
    19621 ccttggcacg tagctccatc acggcgcgac gagcccgctc ggcgaggatt tcatcgcgac
    19681 cgggttcact gccatcgatc tgggccaaga tgctgcgggc ttgggtggtc agcttctcca
    19741 tctcggcgat ggtgttgcga agctggtcga ccaggggaac aaggcgaggt tctagaggcg
    19801 tcacgcccct attttatcgt aacgaacctt gctatcgatc cggaaaatcg ggaatttgtt
    19861 tcaacaattc ggggcggtcg atgtacgggt tgcggttgcc ctgaattgcg tagaccagat
    19921 cgttgtgcgc acgctcgtca gcgtcgggtg ggtcagactc atgccacttg cggagcgttg
    19981 cttcctcgac gttgaagttg tttagcgtaa agtccgccgg acggctgcgg taatagcgcg
    20041 tatagaagta gaagatggcg cgcgcgatgt cgcccttggt cttgtcgtgc ggctcgaaca
    20101 ccgtccgacc gctgtcgtct ttgccaagct tgctcggcga cgtgccgtcg ggattcggtg
    20161 cagtccacgt cacggtcttg acgacgccga atggatagtt ggcgcgacga ccgtttgtat
    20221 ccacgtccga tgcccacagg tggtgcatgt cggactgggc gacgccacgc gcccccaggc
    20281 tctgcggcca ggtgtgttcg gtgttcatct tggccgaatt tgccgtcgag gtgctgttca
    20341 ccggcttgcc gatgcgcccg gtgtacacgc agtcgaccgc gtcgtcgttg tcgggatctt
    20401 cagcgtcggc aaacatcttg tcgcgggccg cgttatacga caggctgcgg gtgccgcggc
    20461 tcacgatgtc cgacagcgcg acgagcagat catttccgct cttgccgctt gtaccgtcgt
    20521 agtatccggt gggaatgctc tgcggctcga cgtgaaatgg caggttgtcg ccttccattt
    20581 cgtcgatcgc ggcaagctct gggccacagg cggcggtgag caggctcaca gagagggagg
    20641 caatggcaaa caggcggcga gcgaaaacga gcggtcgtct ggtcatgttc tcctcgaaag
    20701 caaaccgggc gaaagtgccc gacgagcgga gggctagcac aggacgtacc aaagagggaa
    20761 caggattgac gaaaaccggt gtgaatcgta tagatgttgc taatgaaagt cgttttcaat
    20821 ttgttgcagt gcctgcgaaa tgccccgctg cgagcgcttg tgctgctggg tggtttgctg
    20881 ctcattgttg ggatttttcc gtcggtagcg catgctcaga cgtcgtactt tacgccgcgc
    20941 accatcttgg ctgagttttt cccgcgcagt caggccgtga gctttgagcg ctttgatctg
    21001 actgctgagc agcgccaaaa actgaccgaa gtgctcggct acaccccgcg caagcccagc
    21061 tacacgattt acatcgccaa gaccggcgac caaatcgacg gctacgcgat cattgatgaa
    21121 gaaaaaggcc agcatctgcc gatcacattt gccgtgcagt tcgacgccaa aggcgcggtg
    21181 aaacggcaag agcttatggt ctatcgtgaa cgctacggcg acgaaatccg cgatgcccgc
    21241 ttccgccagc agtttgtcgg caagcacgtc aaaagccagc tgcgcgaagg agaagaaatc
    21301 atcgccatct ccggtgcgac catttcatcg cgcgcgatgg tgattggcgt gcggcgggcg
    21361 cttgttctgc tcgatgagct tgtgttaaag cgcccgatga aacagcccga cacagccaaa
    21421 gccattccgt cttcttgacg cgcccaaagc cggagagcca tgcgccagtt tgcctcctcg
    21481 cggttccgtc tgtggaacct gtcccccgcc gccaagctca tctatacgtt cttttgcggg
    21541 ctgtcgcttc tggcgattgt ttcgtcggtg cttttgtacg aagacttggt cggtccgtcg
    21601 ctgcgatccg gacacgttga acacgtcgag cgctattacg gcaaagaccc cgccaaagcg
    21661 gtcccggccc agccagcgca aaaggcggaa caacccgtgg gcgggccagc catcgcgcta
    21721 cccgaagacg aagccaccgt gcagcttgaa gtcgcgatgc cctatcgcaa gctgctcgag
    21781 gtcacgcact ttcacctgtt tacgattccg gtgttcgtgc tgatcctgac gcatctgtat
    21841 ctgctcacag acgcagaagc gctgggtcag ctcgtctgga tttcgctcgc gtggctgtcg
    21901 gcgctgctgc acattttggc gccgtggctg attcgcttcg gctcgcggca cttggcgcta
    21961 agctatccgc tcagcggctt cggtctgttt gtcgcgtgcg gttggctgac ggtctatccg
    22021 gtctatgtga tgtggcgagg aaaagcccct gccccatccg aaccgacgcc gcccaaaaaa
    22081 gccgaagtgg cccccccgtt ttccgactaa ttacttgcag gtgtcgtcgg tgccgagcgg
    22141 catcacgttg cagcccgccg ggcaaatccg ctgctgcggc acaccgcttg tgcacttgta
    22201 cagacttccg cctgagcagg tccagtactg gccgccgttg taagcgctct ttgtgcagtc
    22261 ccagctgatg ccgccaccgc cgccgtttgc tttggcatgc gcccagccgg cgggaaccca
    22321 gcttccccag cgactgccca ccgatgaacc gttataggac agggttgcag aaccactgcc
    22381 agtgacgttt tgttcgatga tgtcgagcgt gccgccaccg acgcgagtga tcagcgcgac
    22441 gtggccgtag gtgctgtttg tccagaccag catgtcgccc ggcttgggtg ggtgggcgct
    22501 gtcgccattt gcgtagacat caaccttgtc gcgcggcgcg tttgtgagca ggtctttggc
    22561 gttgccatac cagcgcaggc cccagttctt ggtgaagtgg cgcatgacca gctcgacgca
    22621 ctggtactga tagccgtagc tgccgacgcc agcacaggac acgccggtgc cggtgttgcc
    22681 gccgtttgaa tacgcggtgg ttccgttcca cgaagcgagc gcggtgccac aggcgggcat
    22741 cgccgtttcc tcgaagaagc tggggttgac cggctcggtg ctttccagtt cggccccaca
    22801 gcccgactga aaacccagcg ccaaaagaga aaccgtacaa agtgcagcgt tgcgtatgtt
    22861 gtgcataccc agtctcaaag cagaccgtgt gccaagcgct aagccgtcgg caatctgtgc
    22921 gtctttgatg catcgatcca tcagatcgac tcccaggcga tggatcgaaa cagcagctac
    22981 gccacggttc ccagcgccga cccgctttgt tatagagagg cgatgaagca gcgacgtagt
    23041 gttttgggtt tggcagcact tgcggttgcc gtgtcgtttt cgtcttcgct cgtccacgcg
    23101 cagagcgaga catgggccgc cgccctacgc cccggatcag gcgacaagct gacgctgacg
    23161 tggcgacaag ggctgagcag cggcgacttc acccagcttc aggtggcaaa cggcaccgcg
    23221 caggcgcagc gctgtacgcc ggactgcacg ccgattggca agccgacgcc gctgagcgaa
    23281 ggacaaaaag agcagcttct gtcggggctg cgctcggtgg ggctggaatc gctgcgatct
    23341 gccgatgaag ccgagatgac cgccgatcgc gagctgggtc ttgcgctgcc gggcaaaaaa
    23401 gcgcgaacga ttgcgctgcc gaaaaacgac tggcccacga gcagcgacgg ccaaggcatt
    23461 gccaccacgc tcgatgatct gttgcgcacg ctggtcgcca aggccgaagc gcgcccgcag
    23521 gtcaagctgc cgcagaacgc cgatgagctg gcttcgctgc gaatttcact gacggtgacg
    23581 accaacaaac agccgggcgg gcagcttcag cttgaacatg gaacgctgac gattgcgccg
    23641 gaagaaggca gcttgccgcg catgcccagg cccaggccgc agtcccgact tctgtccgat
    23701 gcggaaaaaa cccagcttgt gcaggcgctg caagggctcg atctggatcg cttggaagcg
    23761 tcgattccgc agcgggcgcg gccagccgtc ggggatgatg acgggcgact tttgaccctg
    23821 catcttttgc ccgccagtgc gctgcccgat ccgatcgcga aagtggccaa gcgcggtggt
    23881 gcagtcagca aaactgccgc cgttcctgct gctgcgcagc cgacgaaaca accacgcggt
    23941 ctgcgtcgct atgtggccga ctgggtgcga tcgccagccg gaccggctgt gcgtcttttg
    24001 gcaagctggc tcgctatcca tctgcctacg gcgccttccg gttctgcgga aaaataagcg
    24061 cgcccgtcga aaagttgcag tgacagtttt gggctcctgc ggtgtttgaa aggacaaggg
    24121 cgagcgagtg ctacggcaca agacgcatcg ccccagacct cttcgcctaa ggagtccacc
    24181 atggccgagc ttcgtctttc gcttacccgt cgtctgtccg cgctgaccct cgtttccgca
    24241 tgcagtgcca tcgctggttg cggtgccatc ggcgcccccg cgctgccgtt taagcccgaa
    24301 gaggcgacgg tgcaaaacgt ccgcagcgcg gtcaaatcgg gctttatctc gtgccgcgag
    24361 ctggccagtc gctatcagct cctgcatgac tcgctcgatc cgtcgctgca cgcggtggtg
    24421 acgtggaaca ttgaccttct gcaagatgcc gaccgactgg atgcggtgcc gacggcgcag
    24481 cgcggaaatt tccactgtgt gccgttggtc gtaaaagaca acatccacgt cgccggcatg
    24541 cagagcacgg gcggcgtgtt ggcgctatcg ggtggcggcg tgtcgaccga tgccgaagtc
    24601 gtgctgcggc tgcgcacggc gggcgctttg atcctgggca agaccaacat gccggacttt
    24661 gcactcgacg gaactgggac gcagagctca tacggtggac agacactcaa tccgtacaac
    24721 cgcgcgctca ccgtctatgg gtcaagtggt ggaacggcgg cggcgctcgg ggcgagcctg
    24781 ggcattgttg ggctgggcac cgatacctat ggatcgctga ttcagccggc gagcgcaaac
    24841 ggcctggtcg cgctgcgtcc gacgcaaggg ctgattccaa atgccggggt gatgccgctt
    24901 atgaacctgc aagacacgcc cggcccgatg gcgcgcacgg tcgaagatgt ggcggcgatt
    24961 ctcgaacttc tcgtcgacaa atcgcaggtt ggcaaaggtt cgcaaagcta tacaagtcag
    25021 ctcaagtcga ccggcttgca gggtctttcg cttggctttg atccggcggc actgcagccg
    25081 ctggcgatgc cgatgctgac cccaagcagc gaagtgtcgg atctgtttta caaagcgctg
    25141 gcagacatct cgacggcggg aggcaagacg cggcaggtca cggtgctgac cacgctgttt
    25201 gcgtcgctgc aagcggccac cgacggaagc tttgcctgca tgccggtcga tttcaaagag
    25261 agctttggca actacctggc cagccgatcg gatctgaaaa ccaagacgct ggcggacgtg
    25321 atcgcgacgg gcaagtacag cgactcggcc aaaggctttt tgctgagcgc ccaggcccag
    25381 accgacacaa cgcagaccag cgtggcgtgc cagaagtacc tgacggcgcg cacggcggcg
    25441 caaaacgcga ttgtcgcgct gatggacaaa gagggcatcg accttctggt ctatccggca
    25501 gccaaccagc ccgcgttccc gattggcaca ccaccgagcg gctggttcgg tttccagatg
    25561 ctgtcatcgc ccaccgggct tccatcgctg acggtgtcga tgggaacgac gcccaaaaac
    25621 ggcgcgccga tgggactgac gctgctcgcc cgtccgtacc aagaagccaa gctggtgcaa
    25681 gccgcctacg cctatcagca gcagcgcggc ccacgaccca tcccgcccaa gcagtaacgg
    25741 aacctgcgac ggaagctggg ttacttgggc acccacacgt cgccgcagtt tggaccaaac
    25801 agcgcatcgg tcgaaacatc gacggtcttg ccgggaatat gaccgtgcag ctccactttc
    25861 atgccgccgc cgaccaagca gccttgggcg tcccgctgga tgcccataaa atcgatgcga
    25921 tcggacacgc ttacgtcggt aagccacaga tcctttaggc cctttagttc catccagcca
    25981 tcgaccagcg acggatttgc cgtcacgtcg cctttgtacg tcacgtcgag cgaggtcttg
    26041 gcatacagat cgaacgtgta gtgatcctgc tctcggatgt acgtgccgcg gaagtcgccg
    26101 tctttgcagt tgagggtcag cgtctcgcgg acatgatcac caacaggcat cgtggtcagc
    26161 gacgctttgc agagcgcatc gatcaccagg ttgataagcg tttcggtgcc cggcggtagt
    26221 tccaggcggc tgtcctgttt ggcgtcgcgc aggtgcagtc ccgtcgacag caccgccaga
    26281 acgccctgtt tttcgacatc ggtggcctgc cgtcgttctt cgccacagcc agtcagaagc
    26341 acagacacag acacagccgc cgcagcagca acatgaaaag ctcgcatggt tgggttcctt
    26401 ttggtttgca cgggagaatc caaaagcaat ggccgagcca tgcggccagc gcggacacaa
    26461 aaaaccgccc agcgacgagc ggttccgccc gcgccaaaat gccgcgaacc ggcgttcaca
    26521 tcgctgtgtt taggcgttgg cgagcagata gcgcatcgtg ctcttgacgg cacgacttgt
    26581 gataagcgac gacgcaagcc cgaaagccgg ggtgttgacg aagttcataa acccgacggc
    26641 ggtcttgagc ggcagcgcaa actttttggc ggtcgccgcc tggtattcgc gcaaaaagct
    26701 ggtgtcgggg cgctcgtgtc gagcaatcac atcggcagcc acacacgctg agcgcatcgc
    26761 ataggagatg ccctcccccg tcgctgcgtt tgtgagccga gccgcttcgc cgatccaaat
    26821 ggcgcccggt gaagtaatcg gtcccacgcc ttctgcataa acgatgggat ggcctttgta
    26881 cttgccaacc tgggtcgcat tccgcaggcg ttcaccgacg tgggttgcga tcacttcatc
    26941 aagcagtcgc ttgggatcgg caggatcgtc ggggtcataa cagatgccga cgttgacgcg
    27001 ggtgtcggtc tcggggaaca gccagccgta ccagggcttc acccgctggt cgaagtacat
    27061 ttccaagatg ccctttttgt agggaacacc ctcgtaccag gccatgatcg cagcgatttg
    27121 ccgacgaggc cgcttgtcga ccgaaaagcg cgagtgggct ccatcggcca agatcaacag
    27181 atcggtttcg atggtgtcct tgccatcgct tattccgacc acgcgacccg cgttgtcgcg
    27241 caccatcttt ttggcatgaa agttctgccg aaaagaaacg ccggcgcgct cagcagcaaa
    27301 ggcaatctcg gcatcgaacg tcgaccgcgg gatgatccac gccgcttctt ggttggcgtt
    27361 catttcggct tcattgccac gcggacccac gaagcgcaga ccactgattt tctgcgccag
    27421 cggctgaaag tggtcggcca tgtcgatgtc tttgagcact tcgagacagc gcggacccag
    27481 gccactgccg caggtcttgg gacggggaaa ctcatcgcga tcgagcagcg tcacctcatg
    27541 gcgaccgtgc cgagcaagct ggagcgcaca ggtcgagccg ccagggcccg caccgacgat
    27601 ggcaatcttc atgggatagg ttctccggat aagtcgggcg gcactttagc acaaagccct
    27661 ccgcgacgat tgcacccact tcgtgaaagc aagcggttgc gacattcgga cggctcgacc
    27721 aaagcggact ctcttgtacg atggaatgga tgacatcgag gcacccggtg gaaccagaca
    27781 caaccaaagg aatcggtttt tggcatgggc tgcgcaccgt accgcgcgca gtggatgtac
    27841 taaggaatgt cccgcaagtg ctgcggctct tgctgattcc tcttctgtta acggcgcttc
    27901 tcgacgggct ggcgctgtac tatggctttg gctatctgca ccaactgatc ctgttgcatg
    27961 tgccgcagag cggtctgttt gggctgctca gcggattcct tacgctgctc gccggagtgc
    28021 tggtggtcgc agcgctggcg tggacgtttg gctttgtgta cctgacgctg tgcgagctgg
    28081 tggtcgattc cgtttccgaa cagatcgaag aacatctgac cggacacagc ggcagcacag
    28141 atgactgggg gagcaagctg cgcggcgttc tcatcgggct gggacagtcc cttttgctca
    28201 cgctgattgg gattctgtcg gcgctgattg gaatgatacc gatcgtgggc cccctcatcg
    28261 cgattccgct tggcgcaacg gcactgggtt atggattttt tgccatctct gcggggcgca
    28321 aaggacacag tctgtcgcag cgctggcaaa aaggaaaaga tcatctggct gcgctctgcg
    28381 gactgggtct tcctctcttt gtcatcaacc tgattcccgt cgtgaacttg ctggctctgc
    28441 ccgtcttcat tgtggccgga accctgctgt atctggatac cgaacagcga agctaagtac
    28501 ggcccctcat ccgtccgttt tgtcaccaaa gaccccacgt caagcgaacg agcaaactgc
    28561 cggcttttgg taagccgctt gggtcagcaa aaaatcccgc gaccccttct tacacggtga
    28621 tacgctaggt gattttgaca gagcaaccat gtcagaatcc atgactcgtt ccgcgccggt
    28681 tgggagtcgc ctgccggtga tccgtgtgga aaatccgctg cccgcgctgt ggataggatt
    28741 cgtgatcttc atcctctgga ccatgctggt cctcgcgatg tcgagcactc cgccccatgc
    28801 tgcggcaaac ggagtccttc cgctgcgtga tccggcgctt gaacacggac tggaaaactg
    28861 gtccgcgtgg tccgatcgac ttccgatcaa agcccagacc atctggtccc agctcttgct
    28921 gggcggtagt ctgcttctgg gatgggtgtg gctgctcacc gccggacttg cgtttgtggt
    28981 aaagcccgat ggaattgctg cacgcgcatt ctctcgctgg gctgcgctgt gttcactgct
    29041 gcttatcacc acctatgaac tgcactactc aaaccgattg agtctggcgt atcaggttgg
    29101 ttttgcgctg acgcagtcgg ctctgttgga atttgtactg ttctttccgc agcgcgttcc
    29161 gattctgctc cgctatccgt ggctgcatgt ggtggcgcgg ctcagtgatg tcgtgctgat
    29221 cattttgttt cttctggcat tcctactgga agacaagtgg atcggcgtat tccgcagctc
    29281 gcaagaatat acggccctcg tctgctcggt gatcagtgcg gcggtgatgg tctggcggat
    29341 ctttcatcac accaagcgca gaagatcgca agcgctggta tcagcgacgg cagttttgcc
    29401 tcttcacttg ctgtttgtgg tcgcgatttg cgctgacaaa ctgtgcggtc tacagctcct
    29461 gatgattgca cttcctgccg gttttggcgg cgcgcttggg cttatctatg cgatggttcg
    29521 caacgactta tgggacagcg agctggtagt ccccggaacc ggattgcgtc gggtcttgat
    29581 cgcaattctc agctttatgg gaggactcct tgcgcttgtc cttttgcgtt cccttaccat
    29641 gctgtcggtc caagcacagg tgctgttaac cctggcgctg atcagtgtgt ccgggccgat
    29701 gcacagcttt gtcggaacgt ggctggtcgc ccgactcttc cccgcagaag cactgtatcg
    29761 accaacggtg gacgagctga ttgtccgctt caccgacttg cgtacccggc aggctgtcat
    29821 cgaaaccgtg gaacataccg tgctgtctgt ttgtgcgtgc gagcgcgcca agcttgtatc
    29881 tgttagttcc ccgcggcgcg tctcgcggaa cagcgaaatc catcgaagac agaccgattc
    29941 gacgcaagca cccagcgacc caggacagag ctcgaatgtt ccgctcctgg cacttcccga
    30001 tgcaggagga gagtcaggaa agaccctgtt tcgactgacc caacgggcga ttcggcgggc
    30061 actgcgcggt gctggcgtcg acagcctgcg tcctgaacag attgattccc tgtgcaatgg
    30121 gaatctggtg ttcgtcgatt ccgaacattc aatgcgccga accagtccgg gtctgtgggc
    30181 atggcttttg gttccggtgc gcttccgtga acaagtgatc ggcgtacttg tggtgtcgcc
    30241 caaacagcgc ggtcagctct tcagcagtct cgatcaggag ctgctgcgca ccatcgcgca
    30301 tcaagcggcg ctggccctcg caaatgccat tgcactggaa gaacttgatg agctgcggcg
    30361 cgccgaacac agtgtccacc gtgaaagact cgatcgcgcg atctctacca tcgctgccga
    30421 aatcgcccac gaaattcgtt ttccgatcaa cttcttccgc atgctggtcg aacgtcagga
    30481 acgcgcgctg catcagggaa agccgctgtc cgaacacgac tgggcggaag acctcgacat
    30541 cggtcgcgag gaagttgcgc ggcttgaacg gatggcagac agactccgca agatggcgct
    30601 gtcccgcacc gtgcagccgc gacccctttc actccgttcg attacagagc acgtccgact
    30661 gctgctcgcg gatcgactcg cggcccatac attcaagatg gagctaaaag aaggcatcga
    30721 ggtgcttgct gatcccgacg cgctgataca agtcctgctc aatctgctcg ccaatgcgat
    30781 cgatgccagt accgcagaag gaacaatcgg cctgacttct gtggccgatc ccgacggcaa
    30841 actccggctt gtggtgtggg atgacggacc tggatttgac gcgccggtcg cgcggctttt
    30901 tgagccgtgg tttaccacca aagcgcaagg aaccggactt ggtcttgcca tcacccatcg
    30961 attggtccgc gcgcacggct gggaaattgt cgccgagcgt cgtggatcgc gtacttgttt
    31021 cgacgtggtg attccgctgg gagaatggcg attctgttcg accggagaga tatctctttt
    31081 ggagtctgtc agctccgatg aaaagcagaa aaacgcactc tgataggtcg cgctactcac
    31141 ttgccggggg cgcggaaagc agctcaaacg gatacgtgac cacttgctcg ccgccactcg
    31201 gtggcgcagg aaacaccgcg ctatacatca catcgcggat gcactcacgc acactggtca
    31261 gcttgagcgt actgccttct tcgacaatct tgctttcact caccagaccg ccgacatcgg
    31321 gctcaccgac gatcgtaaac tgcacgaaca gtttcccatg caggtctgga tcgatccgaa
    31381 ttccgtcttc gtaacagtcc ttgatcatag gaatcagcgc gcgcatctga tcccgaatgt
    31441 actctcggtt tagcgcgggc gccgtctcat gcgatggcga tgtctcggtt tttttctgga
    31501 tggtctgaat ctgcgcaagc atctgtgtac gctgcggcag atcgcgccgg gccgaactgg
    31561 gaaccgcgct gggagtcgca ggagcagacg gcgcagccac cactgcagac ggtgcggcct
    31621 ttgtcgcgcc agtgcttccc ttgtgaatca aaagcgcacc accagataca cttgcgacca
    31681 gtgccaacgt gagaaaggtc ttcatgacgg ggactccttt tgtaacaacc ggaaccgatg
    31741 ggtcagtcaa aggaagcgaa cttcccagag gaacaagtgc aagctgccac gcgcggcgat
    31801 caccggcatg acgaacatcc agatcgctac ggagcttttc gagcgacttg gccaagcgcc
    31861 agcgcaccgt cgcggcgggc acttgttggc gcttgccgat ttcttcagcg ctgcatcctt
    31921 caaaataacg aagcagcaca gtggcgcgat aaggctcatc caaacgcagc accagctcag
    31981 acaacattcg ctgcgcttcc gctcgctcaa gcaaacgttc cggcgtgtct tgttcctttt
    32041 ccgtcacctc tctctgcgat gctatttcgc gatggttccg tcgctgcgta ctgcgcgcat
    32101 tcatccgaga cagattccgg accacttcag caagccacgg acgtagcggt ctggtcggat
    32161 cgggaggact gcgcagcgca gcaagccaag tctcttgcgt cagatcgtcc gcttttgctt
    32221 catcacggac caagacatac gccagcttgc ggacccaatc ggcatgggca agcagagact
    32281 ctagcgagag cggctcaggt aaggttgcgg ttttgacacg catcggtttg cctttgcctc
    32341 tctatatgcc gccgcgatca aaagtgttgg cgggtcgatg tcgatcgcta acgtattttc
    32401 ctatctgcgc ttgttctcta gcgattttcg gtagacttcg acggcagtaa ggatctcttc
    32461 gctgcgccag agcgtccaca ccgggtccaa accaggccca cccaccgccg caattctgtc
    32521 gcggccctct ttgcgaagtg ctcgcttggt cgtggcaaat gcgagcggaa cccgacttcc
    32581 cagctcctcc gcaagtgcca gtgcgcgttc caaaagctgg gccgcaggaa ccacttcgtc
    32641 caccaaatga caagccaagg cttccgcagg acgaaacgca cggccacgat agaccagatc
    32701 cgcaaagcat ggacctgacc acgcgctgcg cacgatctcc agcgcagtga cagggaatgg
    32761 aaccccgaca cgaatttccg aaagactgag ctgcgattct gcgtcggtac agatccgata
    32821 gtctgccgtc gctgcgagca cagcaccgcc cgccaccgcg tgaccattta gcgccgcaac
    32881 aacgggaaga tcgatcgaaa acaggcccga aaagacctca tcaaacactg taggaaacag
    32941 cgccgctttg tcttctggat aggcgaatac ctcgaacaga tcgagtccgg cagaaaagaa
    33001 tcgcccctgt cccgtgatca caagcgcgcg gaaatcggga actcgcagcg tcggaatcaa
    33061 cgcccgcagc tccagcagca gatccagatt cagcgcgtgc gcaggtggcc gattcagaaa
    33121 gagcaccgcg gtatttccgt gccgttcaag gcgaatcaac tgtgacatgt ccgcatcata
    33181 caaagacacg ctaggacttg tgcaaaatgg ttccgagcgc cccagcaatg tagacttgtc
    33241 cgtcgaccgc actgatcgct tgcagactgg gaagttcgtc acggctcacg gtcttaaact
    33301 gctgaccatc ataccgcagc acgcttcctt ggactgtgac cgcccacaca tcggatgcag
    33361 acgttccggc gattccgctg atggtatctg tcacgctggt cggaatcaca gcccatgccg
    33421 atccatcaaa gtgagcaatc agtccgtt
//
LOCUS       DAORGN010000028        32612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_503_length_32612_cov_6.718402, whole genome shotgun sequence.
ACCESSION   DAORGN010000028 DAORGN010000000
VERSION     DAORGN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 32612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32612
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_32612_cov_6.718402"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKL17_05240"
     CDS             complement(<1..541)
                     /locus_tag="PKL17_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNK44163.1"
                     /translation="MATKRFSSNERPATAQGGVAVHTLPPSAGPEGGPVRRVLPHSVD
                     AEESVIGGLLFSGRAMTLVADIINSDDFYDAKHEAIYAAIVELEQRTKPIDLITVAEQ
                     MRQSGTLSKLAARGSEAYLAELLNKIATVENIAHHARIVREKATARRLILAASTIQER
                     GYFDEIDVDGYIDQAQQAVF"
     gene            complement(582..893)
                     /locus_tag="PKL17_05245"
     CDS             complement(582..893)
                     /locus_tag="PKL17_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44164.1"
                     /translation="MSEQAGQKQSPLADPEPVPSPAEDEAAAAETRSLLAGLTPLSPP
                     LDLAAKVPQIIERRSRGRFFGRKKLHERIPFVWVSLVMLLVASLLYAVMRLSPALLSS
                     P"
     gene            complement(945..1538)
                     /locus_tag="PKL17_05250"
     CDS             complement(945..1538)
                     /locus_tag="PKL17_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK44165.1"
                     /translation="MDLRDEGELIRRLQQHDERAFQTCVRLYQDKVYGLVYRMLGSQA
                     EAEDVAQEVFVTVFKSIDSFRGDSKFSTWLYRIAANHCKNRIKYLRRRAHKQTAELDD
                     AAERQLFGGGSSQSTLGQQHASPVEALEGLQLSHIVQRAITKLDEEHRLLLVLRDVEE
                     LSYEEIMQVTGLPEGTVKSRLHRARLILKDEIARHTK"
     gene            complement(1541..2611)
                     /locus_tag="PKL17_05255"
     CDS             complement(1541..2611)
                     /locus_tag="PKL17_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44166.1"
                     /translation="MSSHRLVSLGGFCAASLAFLGGLSVANAAEPMVGIRSLAMGDSM
                     RAMATQSEAMLLNPAGLALSQQFQVTGFYSLRLPSLGHFLHASISDSVTNPYFALGLY
                     YNYLRETPRFGFRLRNGGDDLVQQVVGADIVRTGHESGTTLAIPIGQRLTLGASLKYA
                     YYSLRAQLGQDMAPPGQSAADSRIDRENSVDLGSTGSVVTFDFGMTLRPFAGLHVGVV
                     GQNLWPHGYELPTLLGIGLGYQVGDRLLLAVDTVINFTGNEACSDMTQTTCNETTRRT
                     TVRVGGGAEYTIAGKVPLRLGYLYDDNLRAHHATMGLGYVSKDSGFALDVSVRQRVAP
                     GSETVLLFGIRILRDGIAQEGG"
     gene            complement(2620..3582)
                     /locus_tag="PKL17_05260"
     CDS             complement(2620..3582)
                     /locus_tag="PKL17_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44167.1"
                     /translation="MHIESYSEHVALLRSLGTLKQRGLSPRGVLFLALSSQGRIYLAL
                     PSVPEQLTQESLRVGEKMALRWPDKNGDGVPERLYHFDSIHALRDDFYVFNGDRRLKD
                     PGNAMDVGIVMARFIRTCGSSSVFFGCTPHQPGSWLAFGNQTVALHDFGIVEVVPVPG
                     GLLARKMHDDRLYLLTFSDIARTGHLEGWLPVFQSPLGNILLVERRVVDSRLSLSCER
                     GLVEVDVSDLPNVRERSRLPFALPAYDEDAIESLDDDLVEQLTVCPAVLGRLSGEAFV
                     LTRGVPQPWGLRDLSPAELWTAPSGELREIESWLTQIGVAPQAL"
     gene            3631..4731
                     /locus_tag="PKL17_05265"
     CDS             3631..4731
                     /locus_tag="PKL17_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNK44168.1"
                     /translation="MDVLAAEKAKSALLLPEAREHLRRRAARRVRASIRFLRLVVQKF
                     WLSFVLIWVLLTVGTLVIHTYARKDGIEPDYLESLFATYSLFSLESVYPMPKAWAARA
                     VYFGYPLIGLLLVADTVVRATLLLFSRQENQKEWTRVLASTYRDHIILCGLGHVGYRI
                     LERLLHWHLDVVVIEKNTESSFLARTQQMGVPVLLMDARQEESLEAAGLMHARTLVIA
                     TNDDLANVEIALDARRLNPHIRVVLRMFDENIANKLRDAFHLDVAFSSAAAAAPLVAA
                     SVLDLDILGSFTLDGRELFTAKIEIGKESALLGTTIAYLANTHRVMVLSRKCGETPSH
                     LGPAPEEQLTAGDILVLHGELDTLRRLSRLAR"
     gene            4764..5702
                     /gene="bla"
                     /locus_tag="PKL17_05270"
     CDS             4764..5702
                     /gene="bla"
                     /locus_tag="PKL17_05270"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HNK44169.1"
                     /translation="MLWFALWLGLGTATPVDTIDPDAKPSVCDSCAEWNVPHKPFRIF
                     GNSYYVGPDGLSSVLITSDKGHVLIDGALPQSAPQIAENIASLGFRLQDVKWIVSSHA
                     HYDHAGGIAALSRKSGARVAASKRSSEGLRSGSVPADDPQVGYGAEMLFPKVKEVREL
                     SDGEELTLGTLRIKVHYTPGHTPGATSYSWQSCEGTTCLHMVYADSLNAVSAPGFRFS
                     AQPMRITEFEQSIEKVKSLPCDVVISAHPSFSRLFERQDLRRTRKPSDPDPMIDQAGC
                     RSYALDAQDRLHKRLSQEQETEKSAAGKRSAEAPKK"
     gene            5769..6341
                     /locus_tag="PKL17_05275"
     CDS             5769..6341
                     /locus_tag="PKL17_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44170.1"
                     /translation="MKTMRFTFVFGLFLLAGCPQSAKNESSASPSVPIEAKADVAKAA
                     PATEAEVPCTQETQLIPGIPGSPGHLFTSQINPNGSSELAQLMRTMQSELKDARTAIG
                     KGEHVGPLFPRFRKIRCSWPTNAADRNATFDAAAQNYLAAVKALDAAKKEDAAAAYER
                     VLDGCRACHEQSCGGAIPAIEALRMAKADK"
     gene            complement(6354..7919)
                     /locus_tag="PKL17_05280"
     CDS             complement(6354..7919)
                     /locus_tag="PKL17_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44171.1"
                     /translation="MSEPSSVDQTTPPAQASASEDPSVAAIAAPPEDKLTLGQQIAGG
                     LLLVNAIFVLLSEFLIPADPKLGPLFAPGRSVMPAIIDLAIGVSLLRGKPKYRTWALV
                     RVLLGTVLFTAAAWGKDPLMTASQIMIGASLLLFLLGDAGRVRLVAGGAVFFLYSLLN
                     LSGLSATLFGVNPLGTYIQVAVGMLEAAPAGTVTGTKIHYQITAPNPSWRLRTASSTV
                     KENPMADRWLTRPDFDAHVVVIAEREPGQQISLPAMTKVVIENAQRVSKSFVEIDRAP
                     LKTHPQHGVLIHTQATVENIELEYLSGVITTYEHGYQVIAFAPRKSFAKVERELRSII
                     ESFKLPTDEVPTPPADVEPTPTAQVTGQASKYVLSAPQGVWYLRKDEAAKQTNPLSDR
                     WLTRPDLDAHIMVIAELVEGAELDADKYADAVAQNLRSNQSAEIVSIEKVAADPDHGR
                     VLHVKLTSNNLPIEYRIGCFARGEEAWQVLAFASQENYPELEADFEKAIDSFQLPPPR
                     PARIVIPPKARKH"
     gene            8125..8886
                     /locus_tag="PKL17_05285"
     CDS             8125..8886
                     /locus_tag="PKL17_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNK44172.1"
                     /translation="MKRISLLVVPFALSFAACGSPLGKDGAEEGTMGASIDLNQEAQY
                     NEQATVCPGGTKTYGIDVSYYQGTINWSQVKAAGKDFAIVRVSDGTGFMDPKFVTNWK
                     GAKAAGLAVGAYQFFRPTQDATAQADLMVNQLNAVGFGAGDIPPVIDVEVTDGVSGAT
                     LLARVNTWIARVKSRTGRLPILYTSPGFWGGIGSPSTLPYIWVAHWGVSCPNLPAPWG
                     RLRFWQYSSTGKVSGISGNVDIDMYNGSLAELKGL"
     gene            8937..9944
                     /locus_tag="PKL17_05290"
     CDS             8937..9944
                     /locus_tag="PKL17_05290"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNK44173.1"
                     /translation="MNGDAIVSRFRQAVLPSCLVFALACVESGDTPKETDTPADAALA
                     SDDLASESPDAARPDLSPPPIRCTGKPMQIAGTTTVKLMSRGKERTYLLHIPSGYDPQ
                     KPTPLVFAFHGLSDKAPDFLKGIDLEREADKRTMIAIAPQGLGIVSGWNAGNCCGEPQ
                     LFGIDDIGFVRDMIDAAKKELCVDDKRIYAMGFSNGGMFTHRIACELSDLISAVGPVS
                     GSLMFPACKPTRPVSIFHLHGNADPIVGYGGGGTGSFPKVPDMIADWAKRDGCMGMAQ
                     QSYKKGSVTCDSYSTCTDASDVTLCTIDQGKHTWPGSGDGTKDISATPTLLDFFLRHS
                     R"
     gene            9958..11535
                     /locus_tag="PKL17_05295"
     CDS             9958..11535
                     /locus_tag="PKL17_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK44174.1"
                     /translation="MDQTAWVKESESLPELLFATAAKTPNHIAITISDTRIPYAALAT
                     RVRMLAQGFFEQGIRPGDSVALLLPNTPVYVMATYALLQIGAVVLNVSPASQGSELLH
                     ILTQSQAVAIVTLDVFLPGLYRVLDKSPIKRLFLSSLQGLEQKIPPPPGTPPPILIDT
                     LFRPPQLDTAQIRPVADDLAVVQFTSGSTGTPKGVLLTHRNILASVRQSSVWMNAPET
                     PNAGVLCVIPFFHVFGMTIGLHLTVAKGYRMILVPRMDALDLMPILRLIETEKPLSFP
                     AVPTLWAALLSLPGIKAESLQSLHVASSGGASLPAWVQDKYRALTGLPIYEAYGLSEA
                     AGATHSASFPGGSPLGSIGKPLAAIEARLADPSDPSRDVAKGEVGELWVRGDVVMRGY
                     LGQDAETARVLQAGWLKTRDLARCDDEGNYYIVDRSDDVIITSGFNVYPSEVESVLSK
                     HPAVQDVAVFGREDRLRGQVVIAQVVLRSEQNATAEELAQLCRDNLPDYKVPRSILFA
                     DRVPRNPAGKTLRKALH"
     gene            complement(11532..12656)
                     /locus_tag="PKL17_05300"
     CDS             complement(11532..12656)
                     /locus_tag="PKL17_05300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNK44175.1"
                     /translation="MGLPYEAWPVREFILCLDDTEWELLRLRQQLLSRFGERYVVETL
                     LSPAALHHRIDELATRGDPVPLLLLGGQQLADGGLESLIALHQKTPGAAKILFPVGPH
                     DELLPLAVRHAGVCQYLLRPYEDLQLQLCIESVMREQKQVRINETLLHTLREQQKQLD
                     AVQQDVDARVRERTRALEEANRRLTQLAATDGLTGLHNHRYLQEHLVLEVERSLRTGI
                     PLGMLMVDVDHFRQYNNRFGHQTGDEVLRRVARLIAEHRRVNDVVARYGGEEFALLLI
                     NADHRVASNIGERLRARVEAERFPQTHELPGGMLTISVGVASCPMDGTSAASLLQAAD
                     SALFRAKQAGRNRVCIAGEPPLFGDSKAARIQATRVSKSG"
     gene            12974..13615
                     /locus_tag="PKL17_05305"
     CDS             12974..13615
                     /locus_tag="PKL17_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44176.1"
                     /translation="MVTAFGKRFSSVGIFWGLLSLGATVGCETSDPCVLRQAACVDVS
                     LIGKKSDAMGAPIAYRDLSVKVCEGRALAGTACSEGMAPPSCAGELAGTSEPTTLTAV
                     AAYSANVQGLVSFQLPTAFNERTDTPPTEVDTLDAASRKTRLLALRDSDPRAVRILVT
                     QPQGNGAITVWDSRCEEDLFSDDEWSKLKYYRVGKNEYRSVFAPLRKAQTTTP"
     gene            13640..14629
                     /locus_tag="PKL17_05310"
     CDS             13640..14629
                     /locus_tag="PKL17_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNK44177.1"
                     /translation="MTTPLRVGVWGVGVMGERHARVLASQPDAKLIAIYDANRDKARS
                     VAERYGTKVADSPEALLADVDAVSIATPTPLHFSHVEQSLSLGKHVLVEKAITEQVDQ
                     AEVLTAMARRLGKLLMVGHIERYNPVFGELRRLIRENGEPPFAIVFRRLSSFSTSSQA
                     VDVVLDLMIHDVDLMLDLCRGQTVLHHDGIGRIVRTREIDHAVAHVGFAKGPVVSLTA
                     SRVTELKVRSIEVTTPSAFIVADLLRKEIQSYRNSMAAYLTQDGGVASQQNTQIQQVQ
                     VPTVEPLLLEMQDFVRAARTGEQPHVTAEDATQALRLTHALRSQMLAQLVAQP"
     gene            complement(14650..16149)
                     /locus_tag="PKL17_05315"
     CDS             complement(14650..16149)
                     /locus_tag="PKL17_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44178.1"
                     /translation="MRAVSAWLWLWLAAGCGPHSVSTKPSAPPAAKDRSRFLVRVDVR
                     SSEALADYNFAGADLHAWGPRLHAALDRAFDKETTPRTIQIQITMHPVAEATIELSAS
                     PALPSDVADRITASFQNSAPPKTKLVDYTLRVTARVGTGGPGGEASCEPKLLSPHEKR
                     LDAMKRASMAQRLELLRAWSDQEVLPVLAAVMQAADPDFSGVRSIASSLATSQPHAVS
                     VAVLLDKNPAYWRGLLEVTPGNPLLMSSRLFWHLSRGELDLVRLYLKPIYYFAKTDHL
                     AHDYLEQLRDHLVLFYDSLDAQIRVGVALHDESRFDDAIALYESILRQYPCSALARYE
                     QWFAKNARAQKAESAGQPGQIDASLDEWENRRGEIFRCNPMFEIDAVSRGDLEQKRMM
                     RRLELRQLWKDRRVENQDYLRYAEIALDLGEYALAAHLYFLIGTGLPEKEFGDRKVLA
                     HFLFSVEKLGVTSLKELFKGDHGRDFAEIQAAIESRLRKSPTAAKRSGS"
     gene            complement(16302..17669)
                     /gene="mnmE"
                     /locus_tag="PKL17_05320"
     CDS             complement(16302..17669)
                     /gene="mnmE"
                     /locus_tag="PKL17_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNK44179.1"
                     /translation="MSSPTICAIASGIGGGIGIIRISGSDAVAIAARILSPWPAEPES
                     HRLYYGQVLSPLLPDALSSSREVLDHGMFCLMRAPRSYTGEDVVEIHGHGGAVSLRRI
                     VDAAICAGARAAEPGEFTRRAFLAGKIDLTRAEAVASLLSAQSVQAARQATRQLSGEL
                     GLQVGELRRQVTQLLGEMEGLLDFPDLDEDVVILRRLRERLLALANRLGALGGSFRLG
                     GKALSTGLELALLGRTNAGKSSLVNALSESERVLVDAKPGTTRDFVEVRAEWDGIPIT
                     LIDTAGERDDKSELEAQGLKLGQKRYSGADLALVVVDGKIGLGPEEERLIAALPSTLP
                     HLMVWNKTDQVGCLPIPGGAIGVSALNGWGLRQLRQAVLSRLAGSVTAQDELLVTSAR
                     QADALRQASLALARAEDVIASGAAVEMAASEVRIAQSRLGEITGEEVSGDVLDAIFSR
                     FCVGK"
     gene            complement(17676..19493)
                     /gene="yidC"
                     /locus_tag="PKL17_05325"
     CDS             complement(17676..19493)
                     /gene="yidC"
                     /locus_tag="PKL17_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNK44180.1"
                     /translation="MNQERRAMLAIGLCLVVVVAWSMLTGFFQKKAAPANGATQTAGQ
                     AANPTANPTANPTQAANPAAPAASPQVEEVRRIIEQSGLYRAEVTSRGAALTKFELLD
                     PKMWSRDLRWLKLSGGQPTQEVRAQDGPTNLLTSYRPTLLTRLPESGVTLPEPADQQP
                     FVLASDETLPDQTRKLSFVLDLPDVKVTKSYSFPPKSLQIGFVVEIENKKSSPVSHHL
                     EVGLEGYQDPSQKTGMFSPRVVQNEVLWDRAGKRYSLNLEALQNNKTDADDMRGSLRW
                     LGIAQQYFIYGLAMEFGSQVGDKQARAKAEANGAISASVVFSDQLLQPGQKHVYPFTL
                     YAGPKQPEALDGVMVNNQTVGMSTAIEYTWGLGVIARPMLWVLRQFFHMSGSWAIAIV
                     LLTLAVKLLMLWPSLKAMKSAKAMQELKPKMDALKQKFPRDDQRNDLNMAILQLQRDH
                     GVSPLGGCLPILLQMPIWVSLYSMLGNAVELYRVRLWWISDMTASDPYFVLPLLTGVL
                     MFLQTKQSAKTTPVDPQMKMVTTMMPIMFTVFSIFLPAGLTLYILTNTALGMVQQEII
                     NRVNKQGGPAVAAAQTPSVEVVDGPVKLKKTKKSGPKSK"
     gene            complement(19508..19753)
                     /gene="yidD"
                     /locus_tag="PKL17_05330"
     CDS             complement(19508..19753)
                     /gene="yidD"
                     /locus_tag="PKL17_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNK44181.1"
                     /translation="MAESPTWLARGLLSLLRFYQRFFSTALALVSGSGCRFTPTCSEY
                     AVQAIDLHGALRGSKMALLRVCRCHPFHPGGYDPPHR"
     gene            complement(19850..20212)
                     /gene="rnpA"
                     /locus_tag="PKL17_05335"
     CDS             complement(19850..20212)
                     /gene="rnpA"
                     /locus_tag="PKL17_05335"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNK44182.1"
                     /translation="MSSLGYAFPKAERVRKRSEYLRIQGEGRKVVSKHLLAFYLFGED
                     GPPRLGITASRKVGCAVERNRVRRLLRESFRHAKHDLPRGLQLVLIAKPQATCATYSD
                     LCDELSELRRRIRSPHPA"
     gene            complement(20222..20371)
                     /gene="rpmH"
                     /locus_tag="PKL17_05340"
     CDS             complement(20222..20371)
                     /gene="rpmH"
                     /locus_tag="PKL17_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNK44183.1"
                     /translation="MKRTYQPHNLRRKRTHGFRARMASRGGRLVLNNRRRKGRKRLTV
                     KVAAK"
     gene            complement(20565..22028)
                     /locus_tag="PKL17_05345"
     CDS             complement(20565..22028)
                     /locus_tag="PKL17_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44184.1"
                     /translation="MDKRLGHYQIVRMLGSGGMGQVYEAVHDQMKRRAAIKILHKRFA
                     HNRQIATRFLNEAKATSMAEHPGIVHIYEFGQTDDGIAYIVMEYLSGETLRQRLDGQG
                     GKLPPDDVMRLGRQMASALQAAHEKGIVHRDLKPVNIMIVRDPETTGGERAKILDFGL
                     AKIIEPEGGEGITNTGTILGTPAYMAPEQCKSARAADPHSDVYSLGVIFYEMLSGDIP
                     FDADTDAELLSKHMFSDAPPLLSKAPSAGMALAALVHRMLGKDTESRPSAGEVAAELQ
                     QLQASASISTAALSAVSGPASDGGSLRNAVPQAASPKVESAKAVVAPPTPAGPSPDEA
                     VAEATVPKKRRGLLWFFLSLFLCGTGAAVFWQLKGHELLDGYLRKPPVRWSIASIPAE
                     AEVLDENGKVLGKTPLSVERAHGPGTVSLTLRLDGYLDQPVTLPLSTDTAVSEKLQPL
                     PKPQDVPTGFAPSDGGDAGSKGQPSDLAASPATSTWR"
     gene            22250..24439
                     /locus_tag="PKL17_05350"
     CDS             22250..24439
                     /locus_tag="PKL17_05350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNK44185.1"
                     /translation="MRRTSGWLSSFGVSLGTSFLLAATAQATPHPYTVDDMLRLLKVS
                     DPQVTPDERLLFFTQSRARLDRNTWQSSIWVQPLSAAGQPQGPAHQFTFPEHGGKGDG
                     QDMSPRLSPNSGRLAFLSTRDGGPPQLYVMSLAGGEARKLTSLPSGVSDPVWTPDSRG
                     LIFTSSVYADCPPLPAGEDCNRSRAEAQEKNPVLARLADRLFYRHWDHWFDGKRQHVL
                     RVMLPEAGNSQTPVDLTPGDYDAPPIARGGDEPYVIAPDGKELVFVQNRDRDVASSTN
                     SDLWAVPLGKDFVPAGQPRNLTAKNLASDVAPRFSPDGRFLAYLAQRRPGFEADRWEI
                     WLHDRVTGQYRSLTENVDLMAHELAFSDDGQRIFFVTSVKGRRAIMSVDISGKQPPVE
                     ISPGDASELRLVRGQSGPALYFTRSSLQRPPEVFRVDLDAMGKAIGPASQVTRQNDPL
                     LAEVRPGPASQLFARSQDGLTLHSHVVTPPDFTPTQKYPAVILIHGGPQGAWEDQWHW
                     RWNAQMFAGAGYVVLMANPRGSEGFGQKFLDQVSGDWGGKPYDDIMKLVDVLAQQPYI
                     DPKRIGAAGASYGGYMVNWIAGHTDRFAALVSHAGVYDLRSMAGETEEQWFPHWEFGG
                     DPSQSEKYDKFSPSRYADKFQTPTLVIAGERDYRVPMGQALQFFTALQHRKVPSRLLL
                     FPGENHWVVKPLHSRLWYGTVLDWLGRYLGGTKPDAKVLDSATTWAK"
     gene            24560..25294
                     /locus_tag="PKL17_05355"
     CDS             24560..25294
                     /locus_tag="PKL17_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNK44186.1"
                     /translation="MNTAKPSESAPLAALLFAALLVGILQRFVPFGPLVLYPFTLFGT
                     WVHEVGHGITAILVGGKFDHLDIFLDASGIAYSLVKPGWRSGIVAAGGLLAPPLVGAA
                     LLLVGRKAPRPILLLLAAAMILSLVLWVRTLVGWVTVSGLGLLIAALGRFGSQGLRLF
                     FVQLLGLLLAMDFVARIDYLFVAAGEAGGSLRQSDVGGMAKSLGGPYWFWGALIAAIS
                     ALALLAGLWSIWRSAKDVSSRPDLGV"
     gene            25307..26086
                     /locus_tag="PKL17_05360"
     CDS             25307..26086
                     /locus_tag="PKL17_05360"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNK44187.1"
                     /translation="MRHQTQLRPLQVLLTRPLDQSKQTAEWIESEGGIAHIFPCLVVL
                     PTDPRPLQQALVDVSRHSAVAVTSVHAARALIAHWPTEQKLPPLFVLGNKTAQTLAKA
                     GLVPTEIVDGETATARQLAKRLLAAIPATDKPVLFPQAETGREELPLLLAAARRNVEK
                     VAVYQTVPATKEALLPAVQLLRERRIDLWPVGSPKTAQVLLDVLSDEAASLLAGVCVG
                     AIGQTTAQALRDRNLRVDVVAEQPEFEDLLIRLAEAAARRE"
     gene            complement(26087..29098)
                     /locus_tag="PKL17_05365"
     CDS             complement(26087..29098)
                     /locus_tag="PKL17_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK44188.1"
                     /translation="MPKTSPSSSSPTTGEPGASALSSSYVRLARYIYAADLVAGKRIL
                     DLGCADGEGAALLLDRGAQSVVGLDVDAQAVSRGQSRQSARLQLRAVTAEQLLTEGGL
                     LRLVAGMTFDVIFVSGDRSWLKAPGFLSALRWLLSPTGQLLWATCSREAFPHATGAIG
                     YFELLDALEGARLGPVTMLGQSPFYAAALAPFGATDPALILDDTLAPSESPDEYVALC
                     GQAPNRPFEVVRLPRQSLQAGVRVEKQEVRVPDPQVVAERDKLTAELAQVLRDRDGLR
                     DKLSELRAQGDKATAHADSLQAHADRLQAHADRLQAQLDKVSAQLEKSAQQRREAEVH
                     SEGLAERLKVSEEARQRAESIRVELEERERQRARHETEAGEAALLHEKQMRELRTAIE
                     ERDAFVAELEDQARELPRLQERLLQTEKRAEDANRSERQARQKLAEVEGLLLRAKTEM
                     AEQLQKASLPQNLDVRQRELEAARREILVAQAELEEKTHALSQQKASLDEVQRTFEQQ
                     KAQLQKHAEDRAAAEAATVAKLEAERERLSQDAQALRAELTERDERLRLLTKRLETMP
                     VAPRPLEDGVPMPVGDIPTAPVVISSANQARNEEVATLRARTTELEAEVARLKEKVGE
                     AEREAWKQIKARSEAESAAAEVREDTVRKLRDARKLASVEMTRAMEEATKKAVALKEE
                     LARTEAERKEALAQLKQLSTERETVSEQLQTLRAELEALRWASSDGQSGSSVEAEVAR
                     IQRESQAAMQSLRSENARLIEAERTTQRQAEAKNVRIVELEQSVVQLEQALREAREKT
                     ESLGRFDAHGEARPSELGLAHDLQARERAIIDLKAERDALGRLLSEVEREAFARGERA
                     RQLKVSLSERERELETLRVELSDRERRLAQVESQSPQSAELKQREQELVAARRRIDEL
                     LAETSRRVQQDDEVVATALRERARVERLTEVVGQTTRERDEAFHRASELEQRLGQVLA
                     ESDRLRGELLRVSGETAPHILPTDLSKPTRS"
     gene            complement(29212..30822)
                     /locus_tag="PKL17_05370"
     CDS             complement(29212..30822)
                     /locus_tag="PKL17_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNK44189.1"
                     /translation="MKSLIARYHPRSLWIQTLLLFLISVALAAIVTGPMLYGRLESAA
                     LADLEHKGLSTVQTLAKDYELRLAISLRDAVQADPILRKLTSSDDEIRYIAITKGREV
                     IAVSPTTTPKDEIGSHLDQYFASNKNTVIGSDIRRYTESFATQKSPLDSDPLLGGEGK
                     KAPEEPGLLLLGLKTQRSQNKVIQQTVASIAAASLCLFTLVILLYLRWVARRMDRMVV
                     FAKAVAGGQLQQRLSDPLDDELGQLSTALSSMAQRTSDIVGEMLEASRALATAASELL
                     DSSTRQAQNANQQATSITQMGATVAELRQTFGEATSKAESVIELARRSEESSTGGGSA
                     VKESIDGMGHLRDQVQAISQTIQGLLERTDQINAIIEVVNDLAEQSNVLAVNAGIEAA
                     RAGEHGRGFAIVAREVRSLAERSKESTAEVRSILQDIRLAGRDSARVIDEGSRRADRG
                     MQLANAAGDSILQLGDTIVSSSAAAMQIATLTRQQSVGIDQIWQATKGLDQIARETAR
                     GIQQIETASANLKALSSRLTEIVGRYQV"
     gene            complement(30822..31727)
                     /locus_tag="PKL17_05375"
     CDS             complement(30822..31727)
                     /locus_tag="PKL17_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNK44190.1"
                     /translation="MADSAHAWLLFWVLTVWFGLLPSSLRAESVVIVSDARVPQYREA
                     TAAAREALPSAPLLDVTAGDLAALIGQHRPSVVLAVGQKALASAQSAAPELPIVYCLV
                     LGASAPATKTVTGVRLEVSAAEQLALLRLVHPKLKRVAVIYEPRNWTEYITDASKAAQ
                     GLGISLVPRAISDGKQVRAVLSDLLSGVDGLWLLPDPQLITAEMFGFLLVSTIERKVP
                     LFGFFDSFTKAGALASVSPDYTAIGQKAAKLAQSIAQKPETTRIPVPSPVQSPGVLTI
                     NQKTAGQLDVELSQEVLSKARQVFR"
     gene            complement(31763..>32612)
                     /locus_tag="PKL17_05380"
     CDS             complement(31763..>32612)
                     /locus_tag="PKL17_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNK44191.1"
                     /translation="DIMMPKMDGIEAVRAVAAQCPTPVLLTSVIARYPDERARLGSFG
                     FGQVELVEKPMLVGPSAAGDVAALVRRARALAFARGPASRPPRGQTPPSDAAAVAIAA
                     STGGMPALADILRSLPEVFPPLYLAQHLDADFIDSFVRFLRTEIGRPVQVVEDQLPIE
                     PSRVYVAQRAHHLLVRTGQVFVKPAHPDDLSPSADLLFRSVAKSFGKHAYGIVLTGMG
                     RDGAYGLRSLRESGAWTIAQEPSSALVSGMPSAAVELGACCEILTLPQIAARLAALKW
                     KPGGNR"
BASE COUNT         6295 a        10174 c         9717 g         6426 t
ORIGIN      
        1 cgaacaccgc ttgctgggct tggtcgatgt agccgtcgac gtcaatctca tcgaaatagc
       61 cgcgttcctg aatcgtggaa gcggccaaga tcagccgtcg cgccgtcgct ttttcgcgca
      121 cgatgcgggc gtggtgcgcg atgttttcga cggtggcaat cttgttgagc agctctgcca
      181 gatacgcttc actgccgcgc gcagcaagct tggacagcgt gccgctctgc cgcatctgtt
      241 cagcaacggt gataaggtcg atgggctttg tgcgctgctc tagctcgacg atggcggcat
      301 agatggcttc gtgtttggcg tcgtaaaagt cgtccgagtt gatgatgtcg gcgaccagcg
      361 tcatcgcgcg accggaaaac aagagaccac ctatgaccga ctcttcggcg tcgacgctgt
      421 gcggcaaaac ccgtcgcacc gggccacctt ccggaccggc agaaggcggc aaagtatgga
      481 ccgctacacc gccttgtgcc gtcgctggac gttcattgct ggaaaaccgc ttggtagcca
      541 cggcggcact ctagcacgcg ctgcccacag acacgctcag cttacggcga agacagaagc
      601 gcaggggaaa gacgcatcac agcatacaaa agcgacgcga caagcagcat cacaagcgac
      661 acccagacaa acggaatccg ctcgtgcagt tttttgcgac caaagaaacg tccgcgagat
      721 cgtcgctcga tgatctgcgg cactttggct gcaagatcca aaggcggcga aagcggcgta
      781 agacctgcga gcaaagagcg cgtctcggca gccgcagcct catcctctgc cggtgaagga
      841 accggttccg gatcggccaa aggactttgc ttctgccctg cttgctcgct catcgtgatc
      901 ttccgtttcc gtcctgtcgg cgacgcttgc cgacgctgtg tttgctattt ggtgtggcgc
      961 gcgatctcat cttttaggat gagccgcgcc cggtgcagcc gcgatttcac cgtgccttcg
     1021 ggaagcccgg tgacttgcat gatttcttcg taggacagct cttccacgtc gcgcagcacc
     1081 agaaggagtc gatgttcttc atccagcttg gtgatcgcgc gctgcacgat gtggctcagc
     1141 tgaagtcctt cgagcgcttc gaccggactt gcgtgctgct gacctagcgt gctctgggaa
     1201 gacccgccgc caaagagctg ccgttccgcc gcatcgtcca gctccgccgt ctgtttgtgc
     1261 gcgcgccgac gcagatattt gatgcggttt ttgcagtggt tggcggcgat gcgataaagc
     1321 caagtcgaaa acttcgagtc gccgcgaaag ctatcgatcg acttaaacac cgtgacaaag
     1381 acctcttgcg cgacgtcctc ggcttcggct tggctgccga gcatgcgata gaccaggccg
     1441 tagactttgt cttgatacag tcgcacgcac gtctggaagg ctctttcgtc gtgctgctgg
     1501 agccgacgga tcagctcgcc ttcgtcgcgc aggtccatgt ctagccgcct tcttgtgcga
     1561 tgccatcgcg caggatgcgg attccaaaca gcagcaccgt ttcactgccc ggtgcgaccc
     1621 gctgccgcac cgatacatcc agtgcaaagc cagagtcttt gctgacatac ccgagcccca
     1681 tcgtcgcgtg atgagcgcgc aggttgtcgt cgtacaggta gccaagccgc agcggaactt
     1741 tgccagcaat cgtgtactcg gcgccgccgc ccactcgcac cgtcgttcgc cgcgtcgtct
     1801 cgttgcaggt cgtttgcgtc atgtcgctgc acgcttcgtt gccggtgaag tttatcacgg
     1861 tgtccaccgc caaaagcagc ctgtctccca cctgatagcc aagtccaatc ccgagcagcg
     1921 tcggcagctc atagccatgc ggccacaggt tctgcccgac gacgccaacg tggagcccag
     1981 caaacggacg cagcgtcatg ccaaaatcga acgtcaccac gctgcccgtc gagccaaggt
     2041 cgacgctgtt ttcacgatcg atgcggctgt cggctgccga ctgtcccggc ggtgccatgt
     2101 cctgcccaag ctgggcgcgc agcgaatagt acgcatactt gagcgacgct ccgagcgtca
     2161 gccgctggcc aatcgggatc gccagcgtcg taccggattc atgcccggtg cgaacgatgt
     2221 cggcgcccac cacctgctgt accagatcat cgccgccatt tcgcagtcga aaaccaaacc
     2281 gcggcgtctc gcgcaggtag ttgtaatata gcccgagcgc aaaataggga ttggtcaccg
     2341 aatcggaaat cgacgcatgc aggaagtgcc cgagcgacgg caagcgcagg ctgtaaaaac
     2401 ctgtgacttg aaactgctga ctcagcgcaa gtcccgccgg attcagcagc atcgcctcgc
     2461 tttgcgtcgc catcgcccgc atgctgtcgc ccatcgccag cgatcgaatt ccgaccatgg
     2521 gttctgcggc atttgcgacg gaaagcccgc ccaaaaaagc aagcgacgcg gcgcaaaaac
     2581 caccaagcga aaccaggcga tgggaagaca tatgcaacat catagcgctt gtggcgcgac
     2641 gccgatctgg gtcagccagc tctcgatctc gcgcagctcg ccgctcggtg cagtccacag
     2701 ctctgccggc gacaagtcac gcagtcccca cggctggggc acgccgcgcg tcagcacgaa
     2761 tgcttccccg ctcaaccgcc cgagcaccgc cggacagacg gtgagctgtt cgaccagatc
     2821 gtcatccaga ctttcgatcg cgtcttcgtc gtaggccggc agcgcaaacg gcagacggct
     2881 gcgctcgcgc acattgggca gatccgatac atcgacttcg accaggccgc gctcacagga
     2941 caacgacagc cggctatcaa caacacgacg ctcgacgagc aaaatgttgc caagcggcga
     3001 ctgaaacacc ggcagccagc cttccaggtg tccggtccgg gcgatgtcgg aaaacgtcaa
     3061 aagatacagc cggtcgtcgt gcattttccg cgcgagcagt ccgcccggaa ccggcaccac
     3121 ttcgacaatg ccaaagtcgt gcagcgcgac ggtctggttg ccaaacgcca gccagcttcc
     3181 cggttggtgc ggcgtacagc caaaaaacac gctcgacgat ccacaggtgc ggataaagcg
     3241 cgccatcacg atgccgacat ccatcgcatt gccagggtct ttgagccgac ggtcgccgtt
     3301 aaacacataa aagtcgtcgc gcagggcgtg aatgctgtcg aagtgataca gccgttcggg
     3361 aacaccgtcg ccgtttttgt caggccagcg cagcgccatt ttttcaccga cgcgcagact
     3421 ctcctgggtg agctgctcgg ggaccgacgg cagcgccaaa taaatccggc cctgcgacga
     3481 cagcgccaaa aaaagcacgc cgcgaggcga cagaccgcgc tgtttcagcg taccgagcga
     3541 gcgcagcagc gcgacgtgtt ccgagtagct ctcaatatgc atgcgcagag attgctttga
     3601 gcatccaggt tgtcaagcct tgccttacac ttggacgtct tggcagcgga aaaagcaaag
     3661 tcagcgcttc ttttgcccga agcgcgcgag cacctgcgca gacgagcagc ccggcgggtg
     3721 cgcgcctcga ttcgtttcct gcggctggtg gtgcaaaagt tttggctcag ctttgtgctg
     3781 atctgggtgc ttttgaccgt gggcacgctg gtgattcaca cctatgcgcg caaagacgga
     3841 atagagccgg actatctgga aagtctgttt gcgacgtaca gcttgtttag cctcgagtcg
     3901 gtgtatccga tgccgaaagc gtgggcagcg cgcgcggtgt attttggcta tcctctgatt
     3961 ggtcttttgt tggtcgccga tacggtggtg cgggcgactc tgcttctgtt ttcgcgtcag
     4021 gaaaatcaaa aggagtggac gcgggtgctg gcctcaacgt atcgcgatca catcatcttg
     4081 tgcggtttgg gacacgtcgg ctaccgcatc ttggaacggc tcctgcactg gcatctcgac
     4141 gtggtggtga ttgaaaaaaa caccgagtca tcgtttttgg cgcgcaccca gcagatggga
     4201 gtgccggtgc ttttgatgga tgcgcggcaa gaggaatcgc tcgaagcggc gggtcttatg
     4261 cacgcgcgga cgctggtgat tgcgaccaac gatgacctcg ccaacgtcga aattgcgctc
     4321 gatgcgcggc ggctcaatcc acacattcgg gtggtgctgc ggatgttcga tgaaaacatc
     4381 gccaacaagc tgcgcgatgc atttcatctg gatgttgcgt tttcttctgc ggctgcagcg
     4441 gcgcctttgg tcgcggcatc ggtgcttgat ctggacatct tgggaagctt cacgctcgac
     4501 ggcagagagc tgtttaccgc caagatcgaa atcggcaaag agtcggcgct tttgggaacc
     4561 acgattgcgt atctggcgaa cacccatcgc gtcatggtgc tgtcacgcaa gtgcggagaa
     4621 acgccaagtc acctgggtcc ggctccggaa gaacagctca ccgcaggcga tattttggtc
     4681 ctgcacggcg agctagatac actgcgtcgg ctgagtcgct tggcgcgcta agggaattcc
     4741 cccccacaag agaggaacac tcgatgcttt ggtttgcgct ttggcttggc ctgggaacgg
     4801 caactcccgt cgacacaatt gatcctgatg caaaaccttc ggtctgtgat tcctgtgcgg
     4861 aatggaatgt gccccacaag ccgttccgga ttttcggcaa ttcctattat gtcggtccag
     4921 atggactcag ctctgtgctg atcacatcgg acaaaggaca tgtcttgata gacggagcgc
     4981 tgccccagtc ggcgccgcag attgccgaaa acatcgcgag cctgggattc cgtttgcaag
     5041 atgtaaagtg gatcgtaagc tcgcatgcgc actacgatca cgcgggcgga attgcggcgc
     5101 tgtcacgcaa aagcggagcc cgcgtcgccg ccagcaaacg gagcagcgaa ggactgcggt
     5161 ctggcagtgt gcccgcagat gatccccagg ttggttacgg cgctgaaatg ctgtttccca
     5221 aagtcaaaga agtgcgcgaa ctgtccgacg gggaggagct gactttggga acgctgcgga
     5281 tcaaggtcca ttacacaccg ggacatacgc cgggcgcgac cagttattcg tggcagtcgt
     5341 gcgaagggac aacgtgtctg cacatggtgt acgccgacag cttgaacgca gtgtcggcac
     5401 caggttttcg cttttccgcg cagcccatgc ggatcacaga gtttgagcag agcatcgaaa
     5461 aagtgaagtc gctgccgtgc gatgtggtga tttcggcgca tccgagcttt tcgcgcttgt
     5521 tcgaaaggca ggatctgcgc cgcacacgca aaccgagcga tcccgatccg atgatcgacc
     5581 aagcaggatg tcgaagctac gcgctcgatg cccaagatcg cctgcacaag cggctttccc
     5641 aagaacagga aaccgaaaag tccgccgccg gaaaacggtc tgcggaagcg cccaaaaagt
     5701 agagcggttt tctgatcctc gcggagcgct gcatccgtcg gcgtccgctt gtgctaggct
     5761 gcggcgccat gaagacgatg cgtttcactt ttgtgttcgg attgtttctt ttggcgggct
     5821 gtccacagtc tgcaaaaaac gagtcctcgg ccagtccgtc ggtccccatc gaagccaaag
     5881 ccgacgtggc caaagcggct cctgcgacgg aagcagaagt cccctgcacg caggaaaccc
     5941 agcttatccc tggcattccc ggcagccccg gacatctttt cacatcgcag atcaatccca
     6001 acggcagcag cgagctggcg cagcttatgc gcacgatgca gtcggaacta aaagacgcgc
     6061 gcaccgccat cggcaaaggc gaacacgtcg gcccgctgtt tccacgcttt cgcaaaattc
     6121 gctgttcgtg gcccacaaac gcagccgatc gcaacgcgac gtttgacgct gcggcacaga
     6181 attacttggc ggcggtcaaa gcactcgatg cggccaaaaa ggaagacgcg gctgccgctt
     6241 atgagcgagt tctcgacggt tgccgcgcct gtcacgagca aagctgcggc ggcgcgattc
     6301 ccgccatcga agctttgcgc atggccaaag ccgacaaata ggaatcgccc ccgttagtgt
     6361 ttccgcgctt tgggaggaat cacgatgcgg gccggacgcg gtggtggaag ctggaaggaa
     6421 tcgatcgctt tttcgaaatc ggcttccagt tccggatagt tttcctgcga cgcaaacgcg
     6481 agcacttgcc aggcttcctc tccacgcgca aagcagccga tgcggtattc aatcggcagg
     6541 ttgttggaag tcagcttcac atgcaggaca cgtccgtgat cggggtcagc cgcaaccttt
     6601 tcgatcgaga caatctccgc ggactgattc gagcgcagat tctgcgccac cgcatcggcg
     6661 tatttgtcgg cgtccagctc ggcgccttcg accagctcgg cgatgaccat gatgtgcgca
     6721 tccagatccg gacgggtcag ccagcgatcg gacagcggat tggtttgctt ggcagcctcg
     6781 tctttgcgca gataccagac gccttgcggc gccgacaaga cgtacttgct ggcctgtccg
     6841 gtcacttgcg cagtcggcgt cggctcgaca tcagcgggcg gcgtcggcac ttcatccgtc
     6901 ggcagcttga acgattcgat gatgctgcgc agctcccgct ccactttggc aaacgacttg
     6961 cgcggcgcaa acgcaatcac ttggtagccg tgttcatacg ttgtgatcac gccgcttagg
     7021 tattcgagtt cgatgttttc caccgtcgcc tgcgtgtgaa tcagcacgcc atgctgcgga
     7081 tgggtcttga gcggtgcgcg atcgatctcg acgaagcttt tggaaacccg ctgcgcattt
     7141 tcgatcacaa ccttggtcat ggccggaagt gatatctgct ggccgggttc ccgttccgcg
     7201 atcaccacga cgtgcgcatc gaagtccgga cgggtcagcc agcgatccgc catcggattt
     7261 tccttcacgg tggacgatgc ggtgcgcaga cgccagcttg ggtttggcgc cgtgatttga
     7321 tagtgaatct tggtccccgt caccgtcccc gctggggcag cttccagcat gccgaccgcg
     7381 acctggatgt atgtaccgag cggattgacc ccaaacagcg tggcggacag acccgacaga
     7441 ttcaaaaggg aatacaggaa gaacaccgca ccaccggcca cgagccgcac ccgccccgca
     7501 tcaccaagca ggaacagcaa aagcgacgca cctatcatga tctgcgacgc cgtcatcagc
     7561 ggatctttgc cccacgccgc agcggtaaac agcacggtgc caagcagcac gcgcacaagt
     7621 gcccaggttc ggtatttggg cttgccgcgc agaagcgaaa caccaatcgc cagatcgatg
     7681 atcgcgggca taacgctccg acccggcgca aacagcggcc cgagcttcgg atcagcggga
     7741 atcaaaaact cgctgagcag cacgaagatg gcgtttacca aaaggagtcc gccggcgatc
     7801 tgctgaccga gcgtcagctt gtcttctggc ggcgctgcga tagctgcaac cgacggatct
     7861 tcggacgcag acgcctgcgc agggggtgtc gtttggtcga ccgaactggg ctcactcatg
     7921 tgggtacctc gctaagcggt tctacacaca aaacgctgat cgggtccatc accatggggt
     7981 ctagtccccc aggcacaagc gcgcgaagac ctggtcttcc cgtcggaaaa cacccttgtc
     8041 agacttggta tgcaagctgc aatgggttgg gaatagacaa tcccaacttt tggcaaacaa
     8101 gccatacacg gaaaggaaac tgccatgaag agaatctccc tgctggttgt gccgtttgct
     8161 ctgtcgttcg ccgcttgcgg aagcccgctt ggcaaagatg gcgccgaaga aggcacgatg
     8221 ggtgccagca tcgatctcaa ccaagaggcg cagtataacg agcaagccac cgtctgcccc
     8281 ggcggcacca agacgtatgg catcgacgtt tcgtactacc agggcacgat caactggtcg
     8341 caggtcaaag ccgcaggcaa agactttgcc attgtccgcg tcagcgatgg caccggcttt
     8401 atggacccga agtttgtcac aaactggaaa ggcgccaaag ccgccggtct ggctgtcggt
     8461 gcgtaccagt tcttccgtcc gacgcaagat gcaaccgcgc aggccgacct gatggtcaat
     8521 cagctcaacg cggtcggttt cggtgccggc gacattccgc cggtcattga tgtggaagtc
     8581 accgatggcg tgtccggcgc aacgctgctt gcgcgggtga acacctggat tgcgcgggtc
     8641 aagtcgcgca ccggacggct gcccatcctg tacacctcac cgggattttg gggcggcatc
     8701 ggcagcccaa gcaccctgcc ttatatctgg gtcgcacact ggggcgtaag ctgcccaaat
     8761 ctacccgccc cgtggggtcg actccgcttc tggcaatact cgagcaccgg caaggtgtcg
     8821 ggcatcagcg gcaacgtcga catcgacatg tacaacggct cgctcgcaga gctaaaaggt
     8881 ctgtagtttg aaccaatact tgctgcgtgc cggttgcgga tttctataat ccgcgcatga
     8941 acggtgacgc gatcgtttct cgttttcggc aagcagtcct tccttcctgc ctcgtctttg
     9001 cgctcgcctg cgtagaatcc ggcgacaccc ccaaagagac agacacccca gccgacgcgg
     9061 cgctcgcgag cgatgacttg gcgagcgaat cgcccgacgc agcacggccc gatctgtcac
     9121 caccgcccat ccgctgcacc ggaaagccga tgcagatcgc cggcacaacg acggtgaagc
     9181 tgatgtcgcg cggcaaagag cggacgtatc tgctccacat tcccagcggc tacgatccgc
     9241 aaaagccgac gccgcttgtg tttgcgtttc acggactgtc agacaaagcg cccgactttc
     9301 tgaaaggaat cgatctggaa cgcgaggccg acaagcggac gatgatcgcc atcgccccgc
     9361 agggactggg aatcgtatcg ggctggaatg cggggaactg ttgtggggaa ccgcagctgt
     9421 tcggcatcga cgacatcggc tttgtccgcg acatgataga tgcggcaaaa aaagagctgt
     9481 gcgtcgatga caagcgaatc tacgcgatgg gattttcaaa cggcggcatg tttacccatc
     9541 gcattgcctg tgagctgtcc gatctgatct ctgcggtcgg cccggtgtcg ggatcgctga
     9601 tgttcccagc ctgcaagccg acgcgaccgg tatcgatttt ccacctgcac ggaaacgccg
     9661 atcccatcgt cggttacggc ggcggcggaa cgggctcctt cccgaaagtc ccagacatga
     9721 tcgccgactg ggcaaagcgc gatggctgca tgggcatggc gcagcagtcg tacaaaaaag
     9781 gatccgtcac ctgcgattcc tattcgacct gcaccgatgc atcggatgtg accctgtgta
     9841 cgatcgatca gggaaaacat acgtggccgg gatcgggcga cggcaccaaa gacatttctg
     9901 caacgccaac tctgctcgat tttttcctgc gtcacagccg ttaagggaat ccgcacgatg
     9961 gaccaaaccg cgtgggtcaa ggaatcggaa tcgctgcccg agctgctgtt tgccaccgca
    10021 gcgaaaacgc cgaatcacat tgccatcacg atttctgaca cgcggattcc gtatgcggcg
    10081 ctggcgacgc gggtgaggat gctcgcgcag ggatttttcg aacagggaat ccgtcccggt
    10141 gattctgtcg cgctgctttt gccaaacaca ccagtgtatg tgatggcaac gtatgcgctg
    10201 ctgcaaatcg gcgcggtggt gctaaacgta agtccggcga gccaaggcag cgagcttttg
    10261 cacatcctta cgcagtcgca agccgtcgca attgtgacgc ttgacgtgtt tctgcctggg
    10321 ctgtatcggg tgctcgataa gagtcctatc aaacgcctct ttttatcgtc actacaaggt
    10381 ctggaacaga aaattccgcc gccgccaggc accccgccgc cgattctgat cgatacgctg
    10441 tttcgtccgc cgcagcttga taccgcgcag atccggcccg tcgctgacga tctggcggtg
    10501 gtgcagttta caagtggttc gacgggaacg cccaaaggtg tgctgctcac ccatcgcaac
    10561 atcctcgcga gcgtgcgcca gtcttcggtg tggatgaacg caccggaaac gcccaacgcg
    10621 ggcgtgctgt gtgtgattcc gttctttcat gtcttcggaa tgacgatcgg actgcacctg
    10681 acggtggcca agggctatcg gatgattctg gtgccgcgca tggatgcgct cgatctgatg
    10741 ccaattctgc ggcttatcga aacggaaaag ccgctgtcgt ttccggcggt ccccacgctc
    10801 tgggcagcac tcctttcctt gcccggaatc aaagcagaat cgctacagag tctgcacgtc
    10861 gcatcaagtg gcggtgcgtc actgccggcg tgggtgcaag acaaataccg tgcgcttacc
    10921 ggcctgccga tttacgaagc gtatggtctg tccgaagccg caggagccac ccactctgcg
    10981 tcatttcccg gtgggagtcc gcttggatcg atcggaaaac cgctcgctgc catcgaagca
    11041 cgactggccg atcccagcga tccgtctcgc gatgtggcaa aaggggaagt gggcgagctg
    11101 tgggtccgcg gggacgtggt gatgcgcggc tacttgggac aggatgcaga gaccgcgcgg
    11161 gtgctacaag ctggctggct aaagacccgt gacctggccc gctgtgatga cgaagggaat
    11221 tactacattg tcgatcgcag cgacgatgtc atcatcacaa gcggcttcaa cgtgtaccca
    11281 agcgaagtcg aatcggtcct gtccaaacat cccgcggtcc aagatgtcgc ggtctttgga
    11341 cgggaagatc ggctgcgcgg ccaagtcgtc atcgcacagg ttgttttgcg cagcgaacag
    11401 aacgcaaccg ccgaagagct tgcgcagctt tgccgcgaca acctaccgga ctacaaagtc
    11461 ccacgcagca ttctgtttgc cgatcgcgtg ccgcgaaatc ccgccggaaa gacactccgc
    11521 aaagcgctgc actaaccgct cttgctgacg cgcgtggctt gaatgcgcgc ggctttgctg
    11581 tccccaaaca gaggcggctc gccggcgatg cagacgcgat tgcggcccgc ttgcttggca
    11641 cgaaacagcg cagagtccgc cgcttgcaaa agactcgctg cggacgttcc atccatcgga
    11701 cagcttgcga ccccaaccga aatcgtcagc attcccccag gcagttcgtg cgtctgcggg
    11761 aaacgttctg cttcgacgcg ggctcgcagc cgttcaccga tgttgctggc gacgcggtga
    11821 tcggcattga taagcagcag cgcaaactct tccccaccgt agcgcgcgac cacatcgttc
    11881 acccggcgat gttctgcaat cagccgtgcc acccgccgca gcacttcgtc gcccgtttgg
    11941 tgtccgaagc gattgttgta ctgacggaag tgatcgacat cgaccatcag catgcccagc
    12001 ggaatcccag tgcgcagcga tcgttcgact tccaaaacca ggtgttcttg cagataccgg
    12061 tggttgtgaa gtccggtcag tccgtcggtg gctgcaagct gggtcagccg ccggttcgct
    12121 tcttcgagcg cgcgcgtccg ttcccgcacc cgcgcatcaa catcctgctg caccgcatcg
    12181 agctgcttct gctgttcgcg cagcgtatgc aacagcgttt catttatccg cacctgcttc
    12241 tgctcgcgca tcaccgattc gatgcagagc tgaagctgca gatcctcata cgggcgcaaa
    12301 agatactgac agaccccggc gtggcgcacc gccaacggca aaagctcgtc gtgggggccc
    12361 accggaaaca ggattttcgc ggcgcccggc gttttttgat gcagcgcgat gagcgattcc
    12421 agcccaccgt ccgcgagctg ctgcccgccg agcagaagca gcggcacggg atcgccgcgc
    12481 gtcgccagtt cgtcaatgcg atggtgaagt gcagctggcg aaagcagtgt ttcgacgacg
    12541 tagcgttcgc caaagcgcga cagaagctgc tgccgcaggc gcagtagctc ccattccgtg
    12601 tcgtctaggc agagaataaa ttcacgcacc ggccatgcct catatggcag tcccatggag
    12661 gactaagaat ccttatgcct ctgacacagg tgccaagagt cgccgcgcag tagcaccgtc
    12721 gcggacagag gattgggtgc gaagaagaac tcgatgccta gcatcagccg acctgggaag
    12781 gctgctctta tagcacaact acaattgctg cacagcctgg gaaaagtcgg atcataagcc
    12841 ctgttgtcga ggctgctatc gtgagcccat cttgcacttg ggcctgccgc atcctgcgcg
    12901 ctttctgtcg cgcgccacca ccgccgcgac aagtcccgcg ggatggccaa aaactgtgct
    12961 aggttgcgcg gccatggtca ccgcatttgg caagcgtttt tcgtcggtcg gaatcttttg
    13021 gggacttttg tcgcttggtg cgacggtcgg ctgtgaaacc agcgatcctt gtgtgctccg
    13081 ccaagcggct tgcgtcgacg tatcgctgat cggcaaaaag tccgatgcga tgggcgcacc
    13141 gattgcctac cgcgatctgt cggtgaaggt ttgcgaaggt cgagcgctcg ctggcacggc
    13201 atgcagcgaa ggaatggcac caccaagctg tgcgggcgag ctggccggca caagcgaacc
    13261 gacgacgctc accgctgtcg ctgcatattc cgccaacgtc caaggcctgg tcagcttcca
    13321 gcttcccaca gcctttaatg aaaggacgga tacgccgccc accgaagtcg acacgctcga
    13381 cgcggcgtca cgcaaaaccc ggctgctcgc gctgcgagac agcgacccgc gcgcggtccg
    13441 aatattggtc acacagccac agggaaatgg cgccatcacc gtctgggatt ctcgctgcga
    13501 agaagacttg ttctcggacg acgaatggtc gaagctcaag tactaccgtg ttggcaaaaa
    13561 cgagtaccgt tcggtgttcg ctccgctgcg caaagcgcag accacgactc cctgatccca
    13621 tcagcagcgg aggtgcaacg tgacgactcc gctgcgcgtc ggtgtgtggg gcgttggcgt
    13681 gatgggcgaa cggcatgccc gagtgctggc cagtcagccc gacgccaaac tgatcgccat
    13741 ttatgatgcc aaccgcgaca aggcgcgcag cgtcgccgaa cgctatggca caaaagtggc
    13801 cgactccccg gaagcgctgc tggcggacgt ggatgcggta agcattgcta caccgacgcc
    13861 acttcacttt tcccatgtcg agcaaagcct cagcttaggc aagcatgtgc tcgtcgaaaa
    13921 agcgatcacc gaacaggtgg atcaagccga agtcctgacg gcgatggcgc gtcggctggg
    13981 caagctgctg atggtgggtc acatcgagcg ctataacccc gtctttggcg agctgcgccg
    14041 actgattcgc gaaaatggcg agccgccgtt tgcgatcgtt ttccgtcgac tgtcgagctt
    14101 ttcgacatcc agccaggctg tcgatgtggt gctcgatctg atgattcacg atgtcgatct
    14161 gatgctcgat ctgtgtcgcg gtcagacggt gttgcatcac gatggaatcg gacgaatcgt
    14221 ccgcacccgc gaaatcgatc atgccgttgc ccacgtcggc tttgccaaag gtccagtggt
    14281 gtcgctcacc gcttcgcgcg tcacggaact gaaagtccgc agcatcgaag tcaccactcc
    14341 atcggcgttc atcgtcgcgg acctgctgcg caaagagatc caaagctatc gcaacagcat
    14401 ggcggcgtac ctgacccaag atggcggcgt cgcgtcgcag cagaatacgc agatccaaca
    14461 ggtgcaagtg ccgacggtgg agccactgct tttggaaatg caggactttg tccgggcggc
    14521 gcgcaccggc gaacagcccc acgtcaccgc tgaagatgca acccaagcac tgcggctcac
    14581 ccacgcgctg cgcagccaaa tgctcgccca gcttgtggcc cagccatagt cgctgacggt
    14641 ccgcgtcgct tacgatcccg atcgcttggc ggcggttgga cttttcctca ggcgagattc
    14701 gattgcggct tggatttcag caaagtcgcg tccgtgatcg cctttgaaca gctctttgag
    14761 gctcgtgacc ccgagctttt cgacggaaaa caggaagtgc gccagcactt ttcgatcgcc
    14821 gaattccttt tcgggcaggc cggtgccgat caaaaaatac agatgcgccg ccaaggcgta
    14881 ttcccccaga tcgagcgcaa tttcggcgta gcgcaggtag tcctggtttt cgacccgacg
    14941 atccttccac agctgccgca gctccagccg tcgcatcatc cgcttttgct ctagatcccc
    15001 acgcgatacc gcgtcgattt cgaacatcgg attgcagcga aaaatttcgc cccgtcgatt
    15061 ttcccactcg tcgagcgagg cgtcgatttg acccggctgc ccggcgcttt cggctttctg
    15121 cgcgcgggcg ttttttgcaa accactgctc gtaccgggcc agcgccgagc acggatactg
    15181 ccgcaggatc gattcgtaga gcgcgatcgc gtcgtcgaag cgcgactcgt catgcagcgc
    15241 gactccgacg cgaatctgcg catcgagcga gtcataaaac agcaccaaat gatcgcgcag
    15301 ctgttccaga tagtcgtgcg ccaggtgatc ggtcttcgcg aagtaataaa tcggcttgag
    15361 gtagagccgc accaggtcca gttcgccgcg cgacagatgc caaaacagcc gcgacgacat
    15421 gagcagcgga ttgcccggcg tgacttccaa gagccctcgc caataggccg gatttttgtc
    15481 gagcagaacg gcgaccgaaa ccgcgtgggg ctggctcgtc gccaggctgc tcgcgatgct
    15541 gcgcacgccg gaaaaatccg gatcagcggc ttgcatcacc gccgccagca caggaagcac
    15601 ctcttgatcc gaccacgcgc gcagcagctc caaccgctgc gccatcgagg ctcgtttcat
    15661 cgcgtccagg cgcttttcat gcggcgacaa gagcttcggc tcacagctcg cttcgccgcc
    15721 gggaccgccc gttccgaccc gtgccgtcac ccgcagcgtg tagtcgacca gcttggtctt
    15781 gggcggcgca ctgttttgaa acgatgccgt gattcgatcg gccacatcgc ttggcagcgc
    15841 cgggcttgcc gaaagttcga tcgtcgcttc ggccacagga tgcatcgtga tctggatttg
    15901 aatcgtgcgc ggcgtggttt ccttatcgaa cgcccgatcg agtgccgcat gaagccgcgg
    15961 cccccaagca tgcaggtcgg cacctgcgaa gttgtagtcg gccagcgctt ccgagctgcg
    16021 cacatcgaca cggaccagaa aacggctgcg gtccttcgca gcaggtgggg cgctcggctt
    16081 ggtcgacacg gaatgagggc cacaacccgc agccagccac agccacagcc aggcgctcac
    16141 cgcacgcaat ccagaccctt tttgcaccac ccccaaacgc acaaggcccc ccgccttgca
    16201 gcaccctgcg gctgctatct gcgtatgtta cttggctttt tgcgcggcgc gtggactttg
    16261 tcagcgcctt ggacggggga tgattgggtg gttggttgct cttattttcc gacgcagaac
    16321 cgggaaaaaa tggcatcgag cacatcgcca ctgacttctt cgccggtgat ctctccgagt
    16381 cggctctgcg cgatgcgaac ttcagacgcc gccatttcga ccgccgcccc gctggcaatc
    16441 acgtcctcgg cccgcgcgag cgccaagctg gcctgcctga gcgcatcggc ctggcgagcg
    16501 ctcgtcacca gcagctcgtc ctgtgccgtc acactgcccg ccaaccgcga aagcaccgcc
    16561 tgccgaagct gccgaagccc ccagccattg agcgcgctta caccaatcgc tccgcctgga
    16621 attggcaaac agcccacctg atcggtcttg ttccacacca tcaaatgcgg aagagtcgac
    16681 ggcagcgccg cgataagcct ttcctcttcc ggccccagac cgatttttcc atcgacgacg
    16741 acaagcgcca aatccgcacc gctgtagcgt ttttgaccca gcttgagccc ctgcgcttcg
    16801 agctcgcttt tgtcgtcgcg ctcaccagcg gtgtcgatca acgtgatcgg aatgccatcc
    16861 cactcggcgc ggacttcgac aaaatcgcgc gtggtgcctg gctttgcgtc gaccagcacg
    16921 cgctcacttt ccgacagcgc attcaccaag ctcgacttgc cggcattggt gcgcccaagc
    16981 agggccagtt ccagtcccgt cgacagcgct ttgccgccca aacgaaagct gccgccgagc
    17041 gcgccgagcc gattcgcgag cgccaacagc cgctcccgca gccgccgcag aatcaccaca
    17101 tcttcatcga gatcgggaaa atcgagcagc ccttccattt ctcccaagag ctgcgtcacc
    17161 tgccgtcgca gctcaccgac ttgcagaccc agttcacccg atagctgccg cgtcgcctgc
    17221 cgcgccgctt ggaccgactg cgccgacaaa agcgacgcca ccgcctcggc ccgcgtcaga
    17281 tcgatttttc cagccaaaaa ggcgcgacgg gtaaactccc ctggctcggc ggctcgggcg
    17341 cccgcacaaa tcgcagcatc gacgatgcgt cgcagcgaca ccgcgccacc gtggccgtga
    17401 atctcgacga catcttcgcc ggtgtacgaa cgcggcgccc gcatcaggca aaacatcccg
    17461 tgatcgagca cttcccgcga cgacgaaagt gcatctggca gcagcggtga tagcacctgt
    17521 ccgtagtaca gacggtgact ttcaggttcc gccggccacg gtgacaagat acgcgctgcg
    17581 atcgcgacgg cgtcgctgcc acttatgcga atgatgccga tgccaccgcc gattcccgac
    17641 gcgatggcgc agatggtggg agacgacacg cgacgttact tgctcttggg accgctcttt
    17701 ttcgtcttct tgagcttgac cggaccgtcg acgacttcga ccgaaggagt ctgcgccgcc
    17761 gccaccgccg gaccaccctg cttgttcacg cgattgatga tctcttgctg gaccatgcca
    17821 agcgcggtgt tggtcaggat gtacagcgtc aggcccgccg gcaaaaagat gctgaacacg
    17881 gtgaacatga tcggcatcat cgtcgtgacc atcttcatct gcggatcgac gggcgtcgtc
    17941 ttggcgctct gcttggtctg caaaaacatc agcacgccgg tcagaagcgg cagcacaaag
    18001 tacgggtccg acgcggtcat atcggaaatc caccacagcc gcacccggta aagctccacc
    18061 gcattgccca gcatcgagta cagcgacacc cagatcggca tctgcaagag gatcggcagg
    18121 cagccaccga gcgggctcac cccgtgatcg cgctggagct gcaaaatcgc catgttcaag
    18181 tcgttgcgct ggtcgtcgcg cgggaacttc tgcttgagcg cgtccatctt cggcttcagc
    18241 tcttgcatcg ctttggccga cttcatcgct ttgagcgacg gccacagcat cagaagtttc
    18301 acagccagcg tcaaaagcac gatcgcgatc gcccacgacc cgctcatgtg gaaaaactga
    18361 cgcagaaccc acagcatcgg gcgggcaatc acgccaagtc cccaggtgta ctcgatcgcc
    18421 gtcgacattc cgacggtctg gttgttcacc atcaccccgt cgagcgcctc tggctgtttc
    18481 ggtccggcat acagcgtgaa cggatatacg tgcttttgtc ccggctgaag caactgatcg
    18541 ctaaacacca ccgacgcgga aatcgcccca ttggcctcgg ctttggcacg cgcctgcttg
    18601 tcgccgacct ggctgccaaa ctccatcgcc aggccgtaaa tgaagtactg ctgcgcgatg
    18661 ccgagccagc gcagactgcc gcgcatgtcg tcagcgtcgg tcttgttgtt ctgaagcgcc
    18721 tcaaggttca ggctatatcg cttgccggcg cgatcccaca gcacttcgtt ttgtacgacg
    18781 cgcggcgaaa acatcccggt cttctgcgac gggtcttgat agccttcaag cccgacttcg
    18841 aggtggtggc tgaccggcga cgatttcttg ttttcgatct cgacgacgaa gccaatttgc
    18901 aggctctttg gcgggaacga atacgacttg gtgactttga cgtcgggcag atcgagcaca
    18961 aagctcagct tgcgcgtctg atcaggcagc gtttcgtcgc tggccaagac gaacggctgc
    19021 tgatcggcag gttctggcag cgtcacaccg ctttcaggaa gtcgggtcag cagcgtcggg
    19081 cgatagctcg tgagcaggtt cgtcggtcca tcttgggccc ggacttcctg cgtcggctgg
    19141 ccccccgaaa gcttgagcca gcgtagatcg cggctccaca ttttcggatc gagcagttcg
    19201 aacttggtca gcgcggcgcc acggctcgtc acctcggcgc gatacagacc agactgttcg
    19261 atgatgcgac ggacttcttc gacctggggc gacgcggctg gcgcggctgg attcgcagct
    19321 tgcgtcggat tggcggtcgg gtttgccgtc ggattggcgg cttggccggc ggtctgggtc
    19381 gcgccgtttg cgggggcggc ttttttctgg aagaagccgg tcagcatcga ccaagcaacc
    19441 accacaacca agcacagccc gatggccagc atggcccgtc tttcctggtt catgaggaca
    19501 aaagctccta gcggtgcggc gggtcataac cgccgggatg aaatggatga cagcgacata
    19561 cccgaagcag cgccatcttg ctaccccgca gcgcaccatg caggtcgatt gcttgcactg
    19621 catattccga acacgtcggg gtgaagcgac agcccgaacc cgaaaccagc gccagtgccg
    19681 tcgaaaaaaa gcgctgatag aagcgcaata gcgacagcaa acctcgcgca agccaagtcg
    19741 gcgattcagc catggacatg tcaggcaaga ctacctgcgc tgcccgagac ggcaagggca
    19801 gatgcggagt cacgctgcaa ggtgcattcg acatgcggtg gtttcggggt tatgcgggat
    19861 gcggactgcg aatgcggcga cgaagctcgg acagctcatc gcacagatcg gaataggttg
    19921 cacaggtcgc ctgcggcttg gcgatcagaa ccaattgaag gccgcgcggc agatcatgct
    19981 tggcgtggcg aaacgactcg cggagcagcc gtctcacccg gtttctctcg acagcacagc
    20041 caactttgcg agacgcagta atccccaatc gaggcggtcc atcctcgcca aatagataaa
    20101 aggccagaag gtgtttggat acgaccttgc ggccctcgcc ttggattcgg aggtactcgg
    20161 agcgcttgcg aactcgctcg gctttcggaa aagcgtagcc gagagaagac atcaggcctg
    20221 gttatttcgc ggcgaccttg acggtcaggc gcttgcggcc cttgcggcga cggttgttga
    20281 gcaccaggcg accaccacgg gaagccatgc gggcgcggaa gccgtgggtg cgcttacgac
    20341 gaaggttgtg cggttgatac gtacgcttca cagcggatct ccttggatct aggctgaaat
    20401 tgacgtgaca aaacggcccc ttacgcgggg caattagaga aagcttggta ccagcaaggc
    20461 gcgccttctg tcaaggtcgg cgcgcaaaga tcgcgatgtt tccgacggaa aatcacgcac
    20521 acttttgatc gtgcgatgac ctgcccggtt tggaagtgag ctgctcaacg ccaagtcgag
    20581 gttgcaggtg aagcagcaag gtcagacggc tggccctttg atccggcatc accgccatcg
    20641 gatggcgcaa atcccgtcgg gacgtcttgt ggtttgggca ggggctgaag cttttcactg
    20701 accgcagtat ccgtcgacaa cggcagcgtg acaggttggt ccaggtagcc atcgagccgc
    20761 agcgtcagcg acaccgtgcc tgggccgtgc gcccgctcga cggacagcgg cgtcttgccg
    20821 agcacttttc cattttcatc caagacttca gcctcggcgg gaatgctcgc gatcgaccag
    20881 cgcaccggcg gcttgcgaag ataaccgtcc aacagttcgt ggcctttgag ctgccaaaac
    20941 accgcggccc ccgtcccaca aagaaacagc gacaggaaaa accacagcag cccgcggcgc
    21001 tttttgggga ccgtcgcttc tgcgaccgct tcgtcggggg aaggtccggc aggcgtcggg
    21061 ggcgcgacga cggctttggc cgactcgact ttgggtgacg cggcctgtgg aacggcattc
    21121 cgaagcgagc caccatcaga cgccggacca gacacggcac tcagcgccgc cgtgctgatc
    21181 gacgccgacg cctgaagctg ctgcagttcc gccgcgacct cgccagccga cggacggctc
    21241 tcggtgtctt tgcccagcat ccgatggacc agcgccgcca gcgccatccc cgccgaaggt
    21301 gccttcgaaa gcagcggcgg cgcgtccgaa aacatgtgct tggacaaaag ctccgcgtcg
    21361 gtgtcggcat caaacggaat gtcgcccgac agcatttcgt aaaaaatcac accgagcgaa
    21421 tagacatcgc tgtgcggatc agcggcgcgt gcactcttgc actgctccgg cgccatatac
    21481 gctggcgtcc cgaggatcgt ccccgtattg gtaatccctt cgccaccctc gggctcgatg
    21541 atcttcgcca gcccaaaatc aaggatcttt gcgcgctccc cccccgtcgt ctcgggatcc
    21601 cgcacaatca tgatgttgac cggcttgaga tccctgtgga cgatgccttt ttcgtgggcg
    21661 gcttggaggg cggatgccat ctgacggccc aggcgcatca catcgtcggg cgggagtttt
    21721 ccaccctgcc cgtcgagccg ctgacggagc gtttcaccgc tgagatactc catcacgatg
    21781 taggcgatgc cgtcgtcggt ctggccaaac tcgtagatgt gcacgatgcc agggtgctcg
    21841 gccatgctgg tcgctttggc ttcgtttagg aatcgcgtcg caatctgtcg gttgtgcgca
    21901 aaccgcttgt gcaggatctt gatcgcggca cgccgcttca tctgatcgtg cacagcctcg
    21961 tagacctgtc ccatgcctcc tgagccgagc atgcggacga tctggtagtg gcctagcctt
    22021 ttgtccacgt cgatccctga gagctaccca tcgcaaactg aaatgcattt cagattacga
    22081 tttttgaatt ggcattatat atggatcgcg agctgtcagc gtgaggtgat ttcactcgat
    22141 aaaatctctt ctgaagctga caggtgggca aacttgggct gtttagtgtt cattttttca
    22201 gtaattcaaa agataatctg gtgacacttc agactttttt ggacttgtga tgcggcgaac
    22261 ctctggctgg ctctcctcgt ttggtgtttc ccttggtaca agcttcctgc tggcagcgac
    22321 ggcgcaggcg acgcctcacc cgtacaccgt cgacgacatg ctgcgcttac tcaaggtgtc
    22381 cgatccacag gtaacaccgg atgagcgact gctgtttttt acccagtcgc gcgctcgtct
    22441 cgatcgaaat acctggcaga gcagcatttg ggtgcagcca ctgtcagcgg cgggacaacc
    22501 gcaaggacca gcgcatcagt ttacgtttcc ggaacacggc ggcaaaggcg atgggcagga
    22561 catgtcgccg cggctgtccc caaacagtgg tcgactggcg tttctgtcga cgcgggatgg
    22621 tgggccgccg cagctttatg tgatgagcct ggccggtggc gaagcgcgca agctgacgtc
    22681 gctgccgtcg ggtgtgagtg atccggtgtg gacgcccgat agccgcggtc tgattttcac
    22741 aagcagcgtg tacgccgact gcccgccgct gcccgccggt gaagactgca accgctcgcg
    22801 cgccgaggcc caggaaaaaa acccggtgct ggcgcggctc gctgaccggc tgttttaccg
    22861 tcactgggat cactggttcg acggcaagcg ccagcatgtg ctgcgggtga tgctgcccga
    22921 ggcggggaac tcacaaacgc cggtcgattt gacgccgggt gactacgatg cgccgccgat
    22981 tgcgcgcggt ggcgacgagc cgtatgtgat tgcgcccgac ggcaaagaac tggtgtttgt
    23041 gcagaaccgc gatcgcgacg ttgcgtcgtc gacaaactcg gatctgtggg cggtaccgct
    23101 tggcaaagac tttgtcccgg cggggcagcc acgcaacctg acggcgaaaa acctggcgtc
    23161 ggatgttgcg ccgcgctttt ctcccgacgg gcggtttttg gcatatctgg cacagcgacg
    23221 gcctggtttt gaagcggatc gctgggaaat ttggctgcac gaccgggtga cggggcagta
    23281 tcggtcgctg acggaaaacg tcgatttgat ggcgcacgag ctggccttta gcgacgacgg
    23341 acagcgcatt ttctttgtca ccagcgtcaa gggtcgacgg gcaattatgt cggtcgacat
    23401 cagcggcaag cagcctccgg tcgaaatttc gccaggcgac gcaagcgagc tgcggctggt
    23461 gcgcggccag agcggcccgg cgctgtactt tactcggtcg agcctgcaac gcccgccaga
    23521 ggtcttccgc gtcgatctgg atgcgatggg caaggcgatt ggaccggcga gccaagtgac
    23581 gcggcaaaat gatccgctgc tcgcggaggt gcgtcccggt ccggcgagtc agctgttcgc
    23641 gcgcagccaa gacggtctga cgctgcacag ccatgtggtg acgccgccag actttacgcc
    23701 gacgcagaaa tatccggcgg tgatcctgat ccacggcggt ccgcagggcg cgtgggaaga
    23761 tcagtggcac tggcgctgga acgcgcagat gttcgccggg gcgggctacg tcgtgctgat
    23821 ggcaaatccg cgcggctccg agggatttgg gcaaaagttc ctcgatcagg tgtcgggcga
    23881 ctggggcggc aagccgtatg acgacatcat gaagctcgtc gatgtgcttg cgcagcagcc
    23941 gtacatcgat ccgaaacgca tcggcgccgc tggggcaagc tacggcggct acatggtcaa
    24001 ctggatcgcg ggtcacaccg atcgttttgc cgcgctggtc agccacgcgg gcgtctacga
    24061 tctgcgatcg atggcgggcg aaaccgaaga gcagtggttt cctcactggg agtttggcgg
    24121 cgacccgtcg cagagcgaaa aatacgacaa atttagtcca tcgcgttatg ccgacaaatt
    24181 ccaaaccccg acgctggtga tcgcgggcga gcgagactat cgcgtgccga tgggacaagc
    24241 gctgcagttt tttaccgcgc tacagcatcg caaagtgccg tcgcggctgc tgctgtttcc
    24301 gggtgaaaat cactgggtcg taaaaccgct gcacagccgg ctttggtatg gcacggtgct
    24361 cgactggctg ggacggtatc tgggcgggac caagccagat gcgaaagtgc tcgacagcgc
    24421 gacgacctgg gccaaataag aggcattttt tctgcttcat attgcctgcg acatgccaag
    24481 gggtcggcgc gggcgcacct gacttgcgtc ccatgccatt ccgcaggcga ccgcccggga
    24541 agcgggtatg cttaagcgca tgaacaccgc caagccatcg gaaagtgctc cacttgcggc
    24601 gctcctgttt gcggctctcc tcgtcggaat actccagcgt tttgtgccgt ttggtccgct
    24661 tgtactgtat ccgtttacgc tgtttggcac ctgggttcac gaggtcggcc acggcataac
    24721 ggcaatcctc gtcggtggaa agtttgatca cctcgacatt ttccttgatg catccgggat
    24781 cgcgtattcg ctggtgaagc cgggctggcg ttcaggaatc gtcgctgcgg gtggactttt
    24841 ggcgccgccg cttgtcggtg ctgcgctgct gctcgtcgga cgcaaagcgc cgcgaccgat
    24901 tctgcttctg cttgcagcag cgatgattct gtcgctggtt ttgtgggtga gaacgctcgt
    24961 cggctgggtg acggtaagcg ggctggggct tttgattgcg gcgctcggac gctttggctc
    25021 gcaggggctg cggctgtttt tcgtccagct tttgggtctg cttttggcga tggacttcgt
    25081 ggctcggatt gactacctgt tcgtcgctgc gggcgaagcc gggggatctt tgcggcagtc
    25141 agatgtcggc ggcatggcca aatcgctcgg cgggccgtac tggttttggg gtgcattgat
    25201 tgcggccatt tcggcgctgg cgctgttggc tgggctgtgg tcgatttggc gttcggccaa
    25261 agacgtgtcg tcgcgacccg acctgggagt ctagctgccc gtgctgatgc gccaccagac
    25321 gcagcttcgc ccgcttcagg tgctgttgac caggccgctc gaccagtcga aacagacggc
    25381 ggagtggatc gagtccgaag gcggcatcgc gcacatcttc ccctgtttgg ttgttctgcc
    25441 gaccgatccg cggccactac agcaagcgct tgtcgatgtg tcgcgccatt cggcggtggc
    25501 agtgaccagt gttcatgcgg cacgcgcgct gattgcgcac tggccgacgg aacagaagct
    25561 gccaccactg tttgtgctcg gaaacaaaac ggcgcagacg cttgccaagg ctgggcttgt
    25621 gccgacggaa attgtcgacg gcgaaaccgc cacggcgcga cagctggcta aacgactgct
    25681 tgctgcgatc ccagcgacgg acaaaccagt gctgtttccg caagccgaaa cgggccggga
    25741 agaactgccg cttttgcttg ccgcggcgcg gcgcaacgtc gaaaaagtag cggtgtatca
    25801 gacggtgccg gcgaccaaag aagcgcttct cccggcggta cagctcctgc gggagcggcg
    25861 cattgatctg tggccggtcg gaagtccgaa aaccgcgcag gtcctgctcg atgtgctgtc
    25921 agacgaagcg gcgtcgctgc ttgccggtgt ctgcgtgggc gcgattggac agacgacggc
    25981 gcaagcgctg cgcgaccgca acctacgggt tgatgtcgtc gcagaacagc cggaatttga
    26041 agatttgctg atcaggctgg cagaagccgc cgcacgtcgc gagtaactag cttcgcgtcg
    26101 gcttcgagag gtcggtcggc agaatgtggg gcgccgtttc gccgctgacg cgcagaagct
    26161 cgccacgcag gcggtcgctt tccgccagca cttgcccgag tcgctgctca agctccgacg
    26221 cccgatggaa cgcttcgtcg cgctcgcgag tggtctgacc caccacttcc gtcagccgct
    26281 cgacgcgcgc tcgttcgcgc agtgccgttg ccaccacttc atcgtcttgc tggacgcggc
    26341 gactggtttc ggccaaaagc tcgtcgatcc gtcgtcgcgc cgccacaagt tcctgctcgc
    26401 gctgcttcag ctcggcactc tgcgggctct gcgactccac ctgcgccagc cgccgttcac
    26461 gatcggaaag ctcgacgcgc agggtctcca gctcacgctc ccgttccgac agcgacactt
    26521 tcagttgccg cgcccgctcg ccacgggcaa acgcctcgcg ctcgacttcc gacaagagcc
    26581 gtcccagcgc gtcgcgctcc gctttcagat cgatgatcgc ccgctcgcgc gcttgcaggt
    26641 cgtgggccag tcccagctca cttggacgcg cttcgccgtg ggcatcgaac cggcccaggc
    26701 tttccgtttt ttcgcgggcc tcacgcagcg cttgttcaag ctgcaccacc gactgttcca
    26761 gttcgacaat gcgaacgttt ttggcttccg cttgcctttg cgtcgtgcgc tcagcttcga
    26821 taagtcgcgc attttcgctg cgcagcgact gcatcgcggc ctggctttcg cgctgaatcc
    26881 gcgccacttc ggcctcgacc gacgaacccg actgtccgtc acttgatgcc cagcgcagcg
    26941 cttcgagttc cgcgcgcagc gtctgaagct gttccgaaac cgtctcacgc tccgtcgaaa
    27001 gctgcttcag ttgcgcaagc gcttctttgc gctcggcttc cgtccgggcc agctcttctt
    27061 tgagcgcgac ggctttcttg gtcgcttctt ccatcgcgcg ggtcatttcg accgacgcca
    27121 gcttgcgcgc atcccgcagc ttgcgcaccg tgtcttcgcg aacttccgcc gccgccgact
    27181 ctgcctcgga ccgcgctttg atctgtttcc acgcttcgcg ctcggcctcg ccgacttttt
    27241 ctttgagccg cgcgacttcg gcttccagtt ccgtcgtccg cgcccgcagc gtcgcgactt
    27301 cttcgttgcg agcctggttt gccgacgaaa tcaccaccgg cgccgtcggg atatcgccaa
    27361 ccggcatcgg cacgccatct tccagcggac gcggcgccac cggcatggtc tccagccgtt
    27421 tggtcaaaag ccgtagccgc tcgtcgcgtt cggtcagctc ggcccgcagc gcctgtgcat
    27481 cctgcgacag acgctcacgc tcggcttcga gcttcgccac cgtcgctgct tccgctgctg
    27541 cccgatcttc tgcgtgcttt tgcagctggg ccttctgctg ttcaaaggtg cgctggactt
    27601 cgtcgagact cgccttctgt tgcgaaagcg cgtgcgtttt ttcttccagt tccgcttgag
    27661 cgaccaggat ctctcgacgg gcggcttcca gttcacgctg ccgcacatca agattttgcg
    27721 gcaggctcgc tttttgaagc tgctcggcca tttcggtctt ggcgcgcaaa agcaggcctt
    27781 ccacttcggc gagcttctga cgagcttggc gttcgcttcg attggcgtct tcggcgcgct
    27841 tttccgtctg caaaagccgc tcttgcagcc ggggcagctc tcgcgcttgg tcttccagct
    27901 ctgcgacgaa cgcatcgcgt tcttcaatcg ccgtgcgcag ctcgcgcatc tgcttttcgt
    27961 gcagaagcgc cgcttctccc gcctcggttt cgtgacgagc ccgctgccgc tcacgttctt
    28021 ccagctcaac gcgaatcgac tcggcgcgct gtcgggcttc ttcgctgacc ttcagtcgct
    28081 cggccagtcc ttcgctgtga acttccgctt cccgccgctg ctgcgccgat ttttcaagct
    28141 gtgcgctgac cttgtcgagc tgcgcttgca gtcgatcggc gtgcgcttgc agccgatcgg
    28201 cgtgcgcttg cagactatcg gcatgcgccg tcgctttgtc accttgcgcc cgcagctcgc
    28261 tgagcttgtc gcgcaggccg tcgcggtcgc gcagaacctg ggccagctcc gccgtaagct
    28321 tgtcacgctc ggcgacaact tgcggatcgg gcacccgaac ttcctgtttt tcgacgcgaa
    28381 cgccggcttg cagcgattgc cgcggcagcc gcacgacctc gaacggtcga ttgggcgctt
    28441 ggccgcatag cgcaacatac tcgtcggggg actcgcttgg cgcgagcgta tcgtcgagta
    28501 tcagcgccgg atcggtggcc ccaaacggcg ccagcgcagc cgcataaaac gggctctgac
    28561 ccagcatcgt caccgggcca agccgcgctc cctccagggc atcgagcagc tcgaaatagc
    28621 cgatcgcccc cgtcgcatgc gggaacgctt cccgactgca cgtcgcccac agaagctgcc
    28681 ccgtcggact cagcagccag cgcagcgccg acagaaaacc cggcgctttg agccagctgc
    28741 gatcacccga cacgaagatg acatcaaatg tcatcccagc gacgagccgc aacagtccgc
    28801 cttcggtcaa aagctgttcc gcagtgacgg cgcgaagctg aagccgagcc gactgacggg
    28861 actggccgcg cgacaccgct tgggcatcga cgtcgagacc caccacgctc tgggcgccgc
    28921 ggtcgaggag cagggcggca ccttctccgt cggcacagcc gaggtcgagg atgcgcttgc
    28981 cggccaccaa atccgccgcg taaatgtagc gggcgagccg gacgtaagaa gaggacaaag
    29041 ccgatgcgcc gggctcgccg gtcgtcggtg acgaagagga aggggaagtc ttgggcatgc
    29101 agataaaccg tgtgcggtcg ggaatctaac accccaccgc gccaaaaagg cgtgaaaaac
    29161 cgtggtggcg gcatcgttac cacaaacttc gacttcgagg aataaaaccg cttatacctg
    29221 gtaacgtccg acgatctcgg tgagccggct cgacagtgcc ttgaggttgg ctgaggccgt
    29281 ttcgatctgc tggatgccgc gcgccgtctc gcgggcgatt tgatcaagac ctttggtcgc
    29341 ttgccagatt tggtcgatgc cgacggactg ctggcgggtg agcgtggcaa tctgcatggc
    29401 ggcggcagac gatgacacga tggtgtcacc aagctgcaag atcgaatcac cggctgcgtt
    29461 tgcgagctgc atgccgcggt cggcccggcg cgatccttcg tcgatgacac gggccgaatc
    29521 acgtccggcg agccgaatgt cctgcaagat cgagcgaact tcggcggtgg attctttgct
    29581 gcgctcggcg agtgaacgaa cctcgcgagc gacgatggca aagccgcgac catgttcacc
    29641 ggcacgggcc gcttcaatcc ccgcgttgac cgcgagcacg ttcgattgct cggcgagatc
    29701 gtttaccact tcgatgatcg cgttgatttg atcggttcgt tccagcagcc cttggatcgt
    29761 ctgagaaatc gcctgaacct gatctcgcag atgccccata ccatcgatgc tttctttcac
    29821 cgccgagcca ccgcccgtcg aggactcttc cgaccgccgc gccagttcga tcacggattc
    29881 ggctttgctg gtcgcttccc caaatgtttg ccgcagctct gcgacggtcg cacccatttg
    29941 cgtgatgctg gtcgcttgct ggtttgcgtt ttgcgcctgc cgcgtcgagg aatccagcag
    30001 ttccgatgcc gcagtggcga gcgcgcgtga cgcttccagc atttccccga cgatgtcact
    30061 ggttcgctgt gccatcgagc tgagcgcagt ggaaagctgt cccagctcat cgtcgagcgg
    30121 atcgctcagc cgctgctgaa gctgtccgcc cgcgacggct ttcgcaaaaa cgaccatgcg
    30181 atccatccgt cgcgccaccc agcgcagata caaaaggatc accagcgtaa acaggcacag
    30241 cgacgcagca gcaatgctcg ccaccgtctg ctggatcact ttgttctggc tgcgctgcgt
    30301 cttcagccca agcagcagca agcccggttc ttccggcgct tttttgccct ctccaccgag
    30361 cagcgggtca ctgtcgagcg gtgatttttg cgtggcgaag ctctcggtgt agcgacggat
    30421 gtcgcttccg atgacggtgt ttttgttcga cgcaaaatac tggtccaagt gactgccaat
    30481 ttcatctttt ggcgtcgtcg tcggcgaaac cgcaatgact tcgcgaccct tggtgatggc
    30541 aatgtagcga atttcatcgt cgctgctcgt cagtttgcgc aagatcggat cggcctgcac
    30601 cgcatcgcgc agcgagatcg ccagtcgcag ctcatagtct ttcgccagcg tctgcaccgt
    30661 cgaaagaccc ttgtgttcca ggtccgcaag cgccgccgat tcaagccgcc catagagcat
    30721 cggccccgtc acaatcgccg ccagcgcgac cgaaatcagg aagagcagca gcgtttggat
    30781 ccacagcgaa cgtgggtgat agcgagcaat cagcgatttc atcagcgaaa cacctgtcgt
    30841 gccttgctca gcacttcttg ggatagctcc acgtcgagct gtcccgcggt cttctggttt
    30901 atcgtgagga cgcccggact ttgtacaggc gacggcaccg gaatgcgcgt ggtctcgggc
    30961 ttttgcgcga tgctctgcgc gagcttggcg gctttctgcc caatcgcggt gtaatcgggc
    31021 gacaccgacg ccagcgctcc ggctttggtg aagctgtcga aaaaaccaaa cagcggcact
    31081 ttgcgctcga tcgtcgagac cagcagaaaa ccgaacatct cggcggtgat aagctgcggg
    31141 tcaggcaaaa gccacagtcc gtccacgccg ctcagcagat cggacagtac ggctcgcacc
    31201 tgttttccat cgctgattgc ccgcggaacc aaagaaatcc ccaagccctg ggcggctttg
    31261 ctcgcgtcgg tgatgtactc agtccaattg cgcggttcat agatcacggc gacgcgcttc
    31321 agcttgggat ggacaagccg aagcagcgca agctgttccg ccgccgacac ttcgagccgc
    31381 acgcccgtca cggtcttcgt agccggtgcg ctcgcgccga gcaccaagca atacacgatg
    31441 ggtagctcag gcgccgccga ctgggcactc gccagcgctt tttgtcccac agccaaaacc
    31501 accgacggtc ggtgctgacc gatcagcgcg gccagatcgc ctgccgtcac atcaagcagc
    31561 ggagcactcg gcagcgcctc gcgagcggcg gcggtggctt cgcgatactg gggcacccgc
    31621 gcatccgaca caatcaccac cgattcggcg cgcagcgacg atggcaaaag cccaaaccac
    31681 acggtcagca cccaaaacag cagccaagcg tgggcagaat ctgccatatt tcgacgggga
    31741 acactgtggc cgtggccagc tctcatcggt tgccccctgg tttccacttt agcgccgcga
    31801 gccgcgccgc aatctgcggt agcgtcagga tctcacagca ggcccccagt tcgacggcgg
    31861 cactcggcat cccactcacc agcgcactgc tcggctcttg cgcgatggtc cacgcgccgc
    31921 tttcgcgcag gctccgcaga ccgtatgcac catcacggcc catgccggtc agcacgattc
    31981 catacgcatg tttgccaaag ctcttggcga cggaacggaa caggaggtcg gcgctcggcg
    32041 acagatcatc gggatgggcc ggctttacga acacctgtcc ggtgcgcaca agcagatgat
    32101 gcgcgcgctg tgcgacatag actcggctgg gttcgatcgg aagctgatct tcgacgactt
    32161 ggaccgggcg accgatttcc gtgcgcagaa aacggacaaa actgtcgata aaatcagcgt
    32221 cgagatgctg cgccagatac agcggcggaa acacctcggg aagcgatcgc aaaatgtccg
    32281 ctagcgccgg catccccccc gtcgaagccg caatcgcgac cgcggcggca tcagacggcg
    32341 gcgtctgtcc ccgtggtggc cgcgaagcgg gtccgcgcgc aaatgccaaa gcccgcgccc
    32401 gacggaccag cgccgcgaca tcacctgccg cactgggtcc caccagcatc ggcttttcga
    32461 ccagctcgac ctgcccaaaa ccgaagctac cgagccgcgc ccgctcgtcg ggatagcgcg
    32521 caatcacgct ggtcagaagg accggcgtcg ggcactgcgc cgccaccgct cgcaccgcct
    32581 cgatcccatc catcttcggc atcatgatgt cc
//
LOCUS       DAORGN010000029        32532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_506_length_32532_cov_6.242726, whole genome shotgun sequence.
ACCESSION   DAORGN010000029 DAORGN010000000
VERSION     DAORGN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_506_length_32532_cov_6.242726"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1332)
                     /locus_tag="PKL17_05385"
     CDS             complement(160..1332)
                     /locus_tag="PKL17_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNK44192.1"
                     /translation="MHATTDTRTKRLLDLVVLLLDARRPISFAELREQFAEYRSAKPE
                     AGARAFERDKATLLEMGVPVRYVTLEDVEDAPSEGGYIIDRQKYRLPEVVLSPDEVAV
                     LVATAAAVRHQSDFPYRHAAELAVRKLMFDLSGLPARKRLRRQGGDAKTAGPAQLDVM
                     VHLPTTYKSGSLASHLEQIETAIHNRKRLTFEYDAHRGSGWLADGETGGSESSIVGPS
                     MREVDPYGMVYRQGAWLLVGHCHKAQGLRRFRVDRILKLTVAPRPKSPDFDIPADFSL
                     QEQGAISPWRFEREAKVRTKLFVSADTPWIADEDFGPASRSPGEDLGTSGTIVEFDCG
                     NPDYLISRVLSASGGLRVLAPQSLRQRVAETARAIARRNVSTGPSEASLPRAVARS"
     gene            complement(1385..2551)
                     /gene="dinB"
                     /locus_tag="PKL17_05390"
     CDS             complement(1385..2551)
                     /gene="dinB"
                     /locus_tag="PKL17_05390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNK44193.1"
                     /translation="MPVRRILHVDMDAFFASVEQRDRPDLRGQPVAVGGMHRGVVAAA
                     SYEARRFGVRSAIPMSTALRLCPQLKVVPPDFARYKAASEQVFEIFRSVTPLVEGLSL
                     DEAYLDVTENAWNEPLGVEVARRIKKQIVCQTGLTASAGVAPNKFLAKIASGWRKPDG
                     LTVIAPERVERFLSGLPIDALWGVGPVTAKKLRDAGMTRLVDVRSRSEADLSLVVGSF
                     ARTLIHLAHGEDDRPVEPHRERKSVGSETTYPQDLIDRERIADEVRALSKECTDWLSR
                     RQMFAKTVTLKVRYGDFQTITRSETSTVASRDEVQLAQRAIALVDKTEAGRRPIRLLG
                     VSLHGLSDSDSSDTATAETSVAEKSSPGPIQLPLPFVSLAPLAQSPSSNPRASH"
     gene            complement(2566..3759)
                     /locus_tag="PKL17_05395"
     CDS             complement(2566..3759)
                     /locus_tag="PKL17_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HNK44194.1"
                     /translation="MNDETLHKAAQPVSGTASPISSGVPQSGGSQTGSGKIPASRRES
                     VVDDGSGVTVSDLESVRLLLAGSSVLDWHQLAFSDLAQVQRFLRVNEFDPDSPEDMER
                     LENVRAEAVEYLTRHFGYRIPEEVAEGVPAHELLLLASRRARYQTYACVVLKVMHVLQ
                     HLDGREILFKLPVSDDQVFGLVESKVVQIVDQMRSAGLPIVEFAWSRKERDSLITKLL
                     AKRDTLAAHVYDKIRFRLITRRWEDLPAILRELCNRLVPFNYVIPGQSVNTLLPFEKI
                     FDVQPATRRLRPDLQSDAEINIPEVQPNFFSSASYRVINFVADLPLRVGPIMKQVGLD
                     DAQFDPSAVVYVLTEFQVMDAQTAIQNEQGESSHEAYKERQHIQVKARLTRGMRARPS
                     KDAAK"
     gene            complement(4065..4793)
                     /locus_tag="PKL17_05400"
     CDS             complement(4065..4793)
                     /locus_tag="PKL17_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK44195.1"
                     /translation="MAESKPQGTALPGKSGAKDPRRLVEDHIPFVRSIARKLREQVPA
                     IEFDDLVGFGMQGLLEAAQRYDDRHGVAFTTFAYYRVRGAMFDGLRSMGWLPRSEYAR
                     VRFEERANNFIQSQAEREPTANGANPVEERVRDLASALGGLAAVFVTLLDQKDEAQLP
                     DERPPAPEMIERQQMSQRVRRVMARLPDKERFLIEQYYYHDQTLEQVGASMGLSKSWT
                     SRLHARALAMLREELDAESSGKYD"
     gene            complement(4857..5729)
                     /locus_tag="PKL17_05405"
     CDS             complement(4857..5729)
                     /locus_tag="PKL17_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNK44196.1"
                     /translation="MEILGYLLLCLIWSTTWQAIRICLTGYPPLFGASLRFFIAVAVL
                     LGVITARKRSIKLPGGMRQHLALFGAGVVNGLGYALIYLAELKLSGGTTAILCATSPL
                     FTLLVARLFGLEQLVLRRVAGMSVGLFGIAALFWDGQQIGTSQVQAMLLALVAAALMW
                     PIYGALLKRYASDLSPLISTLYFLFYTALSLLLLAPLRGETFPSIGSAPLSAHLALGF
                     LAIFGSVIAWSVYLALLRRLDLSVLSTIGLVQPVFALVVDLAMKEAQLGLRGYVGALL
                     VLVAMALSTWPLRR"
     gene            complement(6079..7365)
                     /locus_tag="PKL17_05410"
     CDS             complement(6079..7365)
                     /locus_tag="PKL17_05410"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK44197.1"
                     /translation="MTSSAKTTAVPSTRSTDTTTSAVEFAKLLRDRRETAGLSRRGLA
                     EASGLSEATIKFIELGKTRPSRRTLLQLLMVPGLGLLSHDLPMPRRELPKGLFSPQST
                     PANCYLAPDYEPIRCLEELERTLRGAGGQIEQSNLYLTPQSAAGYLRFVRSSQREQKR
                     RTQLPFSEIVDGIPGSLTTRGLTVIALGSGDGQPEVRLLQKLIALQKAAVSLWLVDRS
                     EALLATAFAHAGEMLGPALVHRLGVLGDFHHLPRYTDLISPLLERDEQRMYVLLGTLG
                     HLDHEPRFLRDMLAKVTRPDDLLLLDADLLLPGESDLQPLIGKVPVDDTALSDWLFEP
                     FLHAKPRPHHIVLEQRLDVSDSIPDCQTTSIIATVETRGMLPRQFSVLRQRRYRLSSL
                     RTVLAQSGFVTLGTELWQDPQHGTTALLLCQRSDGS"
     gene            complement(7513..8127)
                     /locus_tag="PKL17_05415"
     CDS             complement(7513..8127)
                     /locus_tag="PKL17_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK44198.1"
                     /translation="MGDEEIAEGESKTKRQTSVKRRAAREKDALEYDLQGGTARFMPT
                     QTIQRTPGSKQPDSMQPQPSHRVATAGNTMKTLIVEDDFVSRTIMQEFLKQYGPSHIA
                     VNGKEAIEAVRLALERQEPYDLICLDIMMPEVDGQQALRSIRAHEESAGLPPSKTAKI
                     VMTTALRDFQNISDAYGNHCDAYVTKPINKAQMTAQLKKLGLIH"
     gene            8267..13003
                     /locus_tag="PKL17_05420"
     CDS             8267..13003
                     /locus_tag="PKL17_05420"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNK44199.1"
                     /translation="MSVTLSGGGKRQLLSSGTTDAGGGLDARFVVPQWPQGKYEVEVR
                     AEFGPQIAVEKQTVDLAAAARILLESDKPLYQPGQTVHMRALTMRSQDGHPLNSGTIR
                     FVVTDPRKNRIFQSEKPLSAFGIASTDLPLADELLLGSYHIQAELVSRPDVSAAEPAE
                     RNVEVSRYVLPKIRVSVRAEQSYTEPGKTVKFSVAANYFHGKPVSGGQVTAKAYLNAA
                     SGHLELATLHGKLDELGALSLSVPVPADSTSDNAKLIISATVEDAASQRAESRQELLI
                     AKQPIIVDVVPEAGQLVPQVENQVSVLVARPDGAPVEKAAVSLVVGGAGSTPLAGITS
                     AIGIAQFSLPRLPVNAAGTDQKGCPAASVAVKATVTIPGASAVTLSQCLSVRSEGGLL
                     LRSDRAVYARGDKLTLSVQSAAVQDGLCFIDVVRDKQLQDTLVVPLRNFRGQIAVPLQ
                     DRMAGTVSLLAYVVGPDGRKMRDARLVYVERPSALRVLAEASSTNGPAGTPLHPGQSA
                     RLRLRVVDADSGVGVKAALGLVMVDEALLALRPLRPGLLRAYFSLGQAAKRAAAERQI
                     AAGGMGIEALVEKGGLSALEQDAAKLLLAGAVAPWDDGWESDPWAGRARALTEQKRKW
                     ADALSHYEKGRTIGERVPNEPGKWRYRQDLPSLIHKSGFLTSAQLLDPWRRPLQTDVL
                     IEAAGFPAFDEYAQRLLDERLTTFYRVLWNKLEADLKTGKRQKESDGTVIFSDADIGN
                     LPGVVLLDPWGTQMRVQTRKKPHKIGHLLSKVVLYSAGPDGVFGSKDDLYPSDNTCYH
                     HSCRLSDRDITVVGVSAAQAFSDSKLGCGCGYGAAGGALAGFHRGTVAYARLSEAEGA
                     AVGARKDSVRSSFPETLLYRPEVITNEKGEAEVPLELADSITTWRLLAEAVAQDGRLG
                     SLVMGIPVVKDFFVDLDLPPVVTQHDELAIPVPMYNHLKVGQNVVVTLQQESWFAPLG
                     PLTDTVNIPAGQAAVRYFRIRVLGVGKHTLRLFAQGSAASDAVERPIEVVPDGIEQVV
                     SVQDRLSAQSVQHSVHVPAMMIPSTSEAVLKLYPSPASHVVEGLDSLLRMPHGCFEQT
                     SSTTYPNALILRYLRRARKTTPAIEQKALEYLQTGYQKLLSFEVAGGGFSWFGSAPAH
                     KILTAYGIEEFADMAEVFPVDAKVIERTQKWLASQQQSDGSFVPDRNGIREGAINAVV
                     DDKLRTTAYIALALKRTDRSGVHRRVVDHAREFAAGALRDPKVLEQVSEDPYTLALLI
                     DLLGPSYIPPSAKRPAERPLWEKLWALRKQNEDGRAVYFASVHATPTYGSGKSGVIET
                     TALAASQFHSASLPSQSSSALAYLISAKDSFGTWHSTQATIRSLKALLEQQSGDGTAT
                     SGVLDVYWDGQKHSSLTLVAKEEGLRIVALPPPPPGAHQLSLQYAGKGVFDYQLVTRY
                     YVPRGQSASPPQPASNRAIGISIQTSSLALRRNETLVQTVVLESSQSHMMPLAQVGIP
                     PGFSVEKESLDRLVTSGAIEKYELTPRYVSLYLSALDAGARKVIPIAMTALLPGKVQI
                     PAASVYPYYEPETRSAAAPSVITVQE"
     gene            complement(13044..14147)
                     /locus_tag="PKL17_05425"
     CDS             complement(13044..14147)
                     /locus_tag="PKL17_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44200.1"
                     /translation="MKAVSIAVIGNGRWARALSVRLKHNQKQWRSRIRRAMLYQPPPD
                     LPRLATFAEPSEIAMRVPVIPAKKSGGGGDETLQMTADALMFAAGEVYADSIDIAELR
                     EADLLLLAVPASKVKPLLKSISGILRPDQAMVHAIGSFAPIEGAGRQTMLPVSELIKR
                     ETPITQIGAIAGPALAEDLEEEVPAALVTGSASPDVGAMLYQALHGPQVRVYVSSDLI
                     GVEVARALVGVYAFAVGVAEAMELGPAVRSLLIARGVAEMGRLAAAFGGQEKTMLGPA
                     GLGELVIATERRGAPDFQLGKLIGKGASLGEAARQIDRACDALNMIREGHMHSQRTRL
                     TLPIVSALYRWVGGRHDLRASITDLIEKDLFLL"
     gene            14304..16601
                     /locus_tag="PKL17_05430"
     CDS             14304..16601
                     /locus_tag="PKL17_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK44201.1"
                     /translation="MRRSFSDSLWSAFLCLPLVASCGQDPNPGDNPPVEELPPDPPIC
                     SAPPTALTDVGTCKGPTGDPLPCDEAHRFCKEKYSIPNEAGMTRVELRGNYRPLGWDV
                     GYPLKQSADGKTWEGELYLPHGRESEVKFVVNGNDWRDDPSPLHPGSKVTVRCTMPPI
                     ATCDRQPLGDAESCAETLPPTGDFDWRDTVMYFTFVDRFYNGKRENDKPLQTDKAERP
                     GDYLGGDYAGIIRKIDDGYFTSLGVNALWVTVPFDNPDYLSPINPKADKRQVGGHAYS
                     GYHGYWPIDQWPKSGNRVEEHFGTESELKELVARAHSKGIKVLFDYAMVHVHEQSPLY
                     KSHAAALDGKGLPVLSPDRKTILDGSGKPAWFWPNIALAEDLTPILKNGNTQECICGS
                     GAENPCQWDGTKENPLIGTRCAFQGYLPHWNYDVDDALNYSLDAAVRLLRDTDADGYR
                     ADAVKHISAKWYKGLRARLLSEFPNKRVFVTGETYDFGNRDNLKRFVDSATMLDGQFD
                     FPLRANLASAILSRGNTGKHLPMWELAEFARTNDTYYGCGSMTAPPPVMSPWIGNHDV
                     VRPIHIAENDPLFDEWSDGKNSDPEKERTWTNLPSQPQSPEAYERLANAFAFLLTSPG
                     APLIYYGDEYGLAGGGDPDNRRTLFYADGTLRGSVRSCHQLALFERVKKLGQIRKSHP
                     ALRRGVRKTIEVSDDLWVYEMSTQKAWKTEPAEKVYVAINRSDSPQTACSLPTALLKE
                     LVGDSMIQGPAIVIPPRQARIFIAP"
     gene            complement(16633..17214)
                     /locus_tag="PKL17_05435"
     CDS             complement(16633..17214)
                     /locus_tag="PKL17_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK44202.1"
                     /translation="MSKEDEGKSKRSADEVWDALAPALNETDQTISRLQSELAATRAE
                     LSQLVDIFVGQGTLTPNHRKLLSRVARSAGEPPRVHLQVVADKYVMEGSDVDCDSLLP
                     ICRARCCSLHFPLSRQDLDEGKVRWQIDNPYMIRQNSDGYCTHIDRASKHCTVYEVRP
                     AACRGFDCREDPRIWTDFEKRILAPWPAWVKPD"
     gene            complement(17247..19304)
                     /locus_tag="PKL17_05440"
     CDS             complement(17247..19304)
                     /locus_tag="PKL17_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44203.1"
                     /translation="MMHANRSRNRRWTTLFSLSFLLSACAPSTPTPPDEEPFTAYLGD
                     ADCDVILPGYAYTKTIGGLPPACALPWPSSLYLKEDSTRKTGYTLQFGNRSLPENKQS
                     VAIDPAPYRKRDGYSVGTPILLTIPGLDLSAFASESSIEKSLASDAPLLLLEVTAAGA
                     KRIPYWIESDQYDQESSQPTLFVRPAQILKEATRYVVALRTIKDRAGKTIPPSPAFAR
                     LRDGNTANIAALKDRQARFDKTFQLLEGQGIPRSSLTLAWEFVTASSESMHSDLLAMR
                     DDALANVGGKGPELVVEKVTEYAKTNDGTGRPVDEYIGLELQGSFEVPNYLENVKIGG
                     FDGSQIHRDGKELPRAQGTRKPHFWVRIPHNALGGVPHGLVMYGHGLLNAGDEVDAGY
                     NGKIANDHKLIFFATNLLGMCEDDAIPLLSILSELSRFRSLTDRLHQGMTEWVLLARA
                     MRERLADLPIVQSKKIQVNKSELFYSGISQGGIFGGTFMAVTPDMTYGHLGVPGNNYN
                     TLLQRSTDFNQFIPVLSSAYPDPVNQNVALAAIQLLWDSTDPVSHLRHITAEPFPGNK
                     PHYVLLAPARGDYQVAVVTNENTVRSDIGIKVMANYDKQRKVPLATEQAYPYRGSGVV
                     LYNFGNPWPAAGNQHPQDSFGDPHSKPRRLDAHSQQMVNFFRTGEIIDVCGGDGCTPT
                     LAR"
     gene            complement(19369..21489)
                     /locus_tag="PKL17_05445"
     CDS             complement(19369..21489)
                     /locus_tag="PKL17_05445"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNK44204.1"
                     /translation="MDRDTSSPAAQNRQHTLPFALTCFVSALLLFLVQPLLGKFILPW
                     FGGTPGVWTTAMLLFQALLLGGYLYAHICTRLLQRGLGRWVFSLYVALVVLSLGFLPI
                     IPSDALRPHGSDAPIPRILLLLVRTVGVPYFLLAATGPLSQAWLSVRGGSPYRLYALS
                     NAGSLLALFGYPLVMEPLFPVTTQAKLWSLGFGLLLPLWLLCAYQGLLKPALQAALPT
                     SSSPDLPPQADAERTQNKTETAMSILLPMLACGMLLSTTNHLCEDVAVIPFLWVLPLA
                     IYLLSFILTFESERFYRRKRFAVLTLLAVALLLVSESFPSLLGPVLGQYAYVRSLFLS
                     LVLLFSTCMICHGEVAQTRPGAEHLTGYYLRISLGGVLGGVLLAVLSPLLLSHTAEWY
                     GTLLGCYLIAIFVRVIDEQDWTARIRTLFPAALGTVGILLALQHQHRRHPDTTILASV
                     RNFYGEVQVHDRVPNRPDEHDRAMFHGGTIHGVQYQSAKFRRDPITYYDEASGIGQVF
                     AQLENREHLHAGIVGLGIGTLATYARPGWRFRFYELNPVVEKLAQTYFTYLSSCSGTV
                     SVVLGDARLSLAQETEQMFDLLVLDAFSGDAIPTHLLTQEALATYQRHLKPDGALVIH
                     ITNRYLDLAPVVRGLAEKGRFTVTRVRSPQDDPHEIYAADWMILSRSEALVSALRPHS
                     SPIGTRPPILWTDESSPLLNLLLR"
     gene            complement(21510..23996)
                     /locus_tag="PKL17_05450"
     CDS             complement(21510..23996)
                     /locus_tag="PKL17_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNK44205.1"
                     /translation="MPKQLHHDPEPSPFHAVAKPAHTTDGTATTQHPAAQPQIESGKP
                     HSMWDAVAPIAAQGVASYLSAHPGAVKALQGWGAKIDGWLHKAGDFLSHLGGTSDAGH
                     HGSEATPHPAPALAAAKAAGAKAEPPKAQEPEPAPPVKTDGVAAHAEVTPGHESASSP
                     KAPSSEAQADKAGSDKNADPWRSVLKGQTRLKAGDSGAAVAKMQSELSQAGFAVPASG
                     VFDEATKKALLKFQNTYHIQATGELGPQTSNGLEEVVGWKGVASGKDILEPGEQGPSV
                     TMLQRQLVAAGYKVDLSGVYDPKTSAVVKELQRSKGWGPDGKVGPLTVGAIKELQHED
                     SASTGPDNANHVPSDKEGRAVYDDNKARLRDLKLQLSQGQKGDMDLFLRNFDKNEARY
                     DSVAQKANMPPELIAALHWRESSGDFGTYLHQGDPLGRPPRHWPTNIPTFYKWEDAAS
                     DALHRQKDAQQMFHIGRDTTDMAALASYSEYYNGLGYHYKDKPSPYAFSGTNQYQRGK
                     YVADGHYDPSAKDSQLGTVAMIEALREHEHKTQAPKPAPEAGDPRSNISLTMAAHPWL
                     EDKTQAPKPDGAKPATEAPAQAPSKVPVPAHAPVPTPTEKPQAHAQPGPVAEPQTEAA
                     KYEYYRHAVEGMGGQFDARPEQRNIVGVRGWMNGHPVPNVKDQYNDTLAVLWNDKLGK
                     HHVEKYNATVDPGAFPKYYNPAGDANLMEGQYDYVLGDHKGHTALNQGEAVNVWRDAN
                     KDGIRQKNEKSESGWFGINVHAGGVEPQVSNWSAGCQVIRGGWGGDPWQNFIHQMKAD
                     PDHRYKYTVIDSQHMPHQQPEDPTKQQGHG"
     gene            24172..25686
                     /locus_tag="PKL17_05455"
     CDS             24172..25686
                     /locus_tag="PKL17_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44206.1"
                     /translation="MSSSQPPRALWAHPLQLVDDLSLPAARLRLGYLLLMLGMLPVLL
                     LLGTARFPSLARLAAWVGLLLSGLPALHIVTSKRARPSAVAGLSLMIALCYFLATLHE
                     ERLLLRWGEARITGGAVTLAMAYAAVAIPVLSLGTWLGGALGVGRVFPKLRLPLPERA
                     LLWSGVLIVSWSLLLDVLRMRGLVPRSQQLISVLSALAPIDLGYALVLLPSLRPGRER
                     LGWLFWGLTVLSSLVALMSGSLAPMVQPLLTYLLGWLLIRRRLRVWPVVLGTLAVLLF
                     QPVKGEFRAKVWDREVRLSLFERAQLFVDLSARHWLGGETDKTVDRERSVKVAAARTA
                     SALQLSHIVEMTPAAIPYQNGATYRYFRYALLPRVFFPDKPSAQYADVWAAVMYGYTT
                     PSGTQHVMVGLPQLAEAYLNFGVFGGLGVVLLCGLLLRIADEMLAHRDAGCGAVALYL
                     FFAQQVMRTFEGSAGQFWGGVLQQFGLYALLLWAYGALLRAASNRPLSSVPSSV"
     gene            complement(25623..27125)
                     /gene="miaB"
                     /locus_tag="PKL17_05460"
     CDS             complement(25623..27125)
                     /gene="miaB"
                     /locus_tag="PKL17_05460"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNK44207.1"
                     /translation="MNGLSDSHSASSAQPLPHHASETGLGAARARRVFIESFGCQMND
                     YDVERMTEVLRRDGFERTDVVEEADMIVVNTCSIREKAENKAASAAGRYQKLKERNPN
                     LLVAIGGCVAQQEGDALLKRIPSADLTFGPDQIPSLGQHFRALRPQLESWPKSAPKAG
                     RYKLRLAQTEVIDVEDYQFLDADPRPSDVKVTALVTIQKGCDNFCAYCIVPRTRGREV
                     SRPADQVVKEVTRFVELGAREVTLIGQNVNAYHAIGTPDGEDFAELLRRVDAVPGLLR
                     LRFTTSHPHYFTRKVADAFRDLRTLKPWLHLPVQAGSTNVLAAMAREYTREAYLEKIA
                     YLREVCPDISLSTDIIVGYPGETEADFDQTMSLLSEVEYDSIYSFAYSRRPGTPAVEL
                     PDDVPAEEKSRRLTALQSLQKQITGKRLQRLVGRTEEILIEGRARANPSQLCGRTGGN
                     HTVNVELPEGQSGDALTGTLVPVLITQAKVHTLLGTLDSGRFDAARSSAP"
     gene            27266..29575
                     /locus_tag="PKL17_05465"
     CDS             27266..29575
                     /locus_tag="PKL17_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44208.1"
                     /translation="MTRRLGFTLFSALLCIGNAAHADKPVRTWNYLTTGNGHGFQFFD
                     TNKNKITHFLEAPYRYLRPRSDPRSDGYGRRNLAYDFYFGVKSGSGGGWLNTGTAGDA
                     SYLEETNIIKVPITLSGVSAETYYFAPFGFEGNAMIAVLKAPGATDGFALFNFHLGDG
                     TPDTPNANSERLKVLSDGKSIAESGPGGGAMIYVPITALAHADCDSAWSKVNSGQSLG
                     DKSNCSGNDVVPAFQSSLDGGYMAVATLFAENESDAETLLAQFKTWLNGRGAQKVLTD
                     ALAEWTAWRKPLPTGLALCTDDEKKLWRQSEAVLRMGQVRQPNTSSRKNFGMMLASLP
                     PGEWHTGWVRDGLYATVALARMGHHTEAKMGLDFLLNAEPVSKYSSYVSGASYRISVC
                     RYFGTGEEEADYSGQQTPNVEIDGWGMTLWAARQYVEASGDTAWLSEKTRLGPSVYDA
                     LYSGVAEALRKNTESSGIAKADSSIWEVHDENKKHFAYTTLATIRGFCDFAQLATKAG
                     KGSDATTYRGLASKARDGFLQSFVDREGAIAGSVEELAQNQYLDGAVVEAFTWNVLKD
                     SEYVGRTGKATLDVLGRLRVESGGFKRNDDGKSSYDNNEWIMIDLRMADALWRAGRES
                     ESAGLTQMIIDKASANYYLLPELYNAVRADGAVGKYIGSIPMVGYGGGAYVLTMLDRS
                     GLIEPNDCGDGMGNKSSGRALKCSDPKVTPTNPTNPSAPTADELPYENACLCGIGPRH
                     RRVSPLVLSAGLVLGTLLWRRRRARGVKA"
     gene            29572..31095
                     /locus_tag="PKL17_05470"
     CDS             29572..31095
                     /locus_tag="PKL17_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44209.1"
                     /translation="MMSRSLRPALFAFSLLSAVPSVWADEPADAVAAAAPAPVAIPQV
                     EAEPPAKSLPVPAVPAAADARPGMGVTIGPVELKATGYLRAPLRLSFRSRGDSVKEGE
                     GQYNIHTPWLVDDDYFRSGFQYLRTQESDWSELYFSAQSKYLTADVALMGSLFTDWAR
                     PLLDRQWGIAQGSVTFRYEALGPKLKFRMHVRAGSFWDRFGWLENYDTYVFGRTHQMG
                     GQVRFEFETRPVTFWLLQGVGAHLDAIEANQGLTLMNYLHLGASVYKVAHLGFYFIDT
                     LAQDKRQLKEVQDASMRVVGLDLRLNPFFGNFYLAGSIITSTQAQYMSPVLEVMHSWG
                     GRGFTENYLGTDKSDGTGSLRSLAGEYTFSLRRVLSAVAPEKAGFLRGGDLQWKWFGL
                     VAYTLSKQVDPDPSINRDGRLSFKWGTELFYQPMAFAFVALRYDRVILDVQDDASAIR
                     VISPRIGATVNWLLGAQIFLQYSRYFYGERVRLRPGQVALETLPDADVVKLQAQVSF"
     gene            31159..32112
                     /gene="gshB"
                     /locus_tag="PKL17_05475"
     CDS             31159..32112
                     /gene="gshB"
                     /locus_tag="PKL17_05475"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNK44210.1"
                     /translation="MRVLVIIDPPASLRPRSDTSVALMDECLQRGHQVFVCELHDLSL
                     RDCKPYALSQRVTGTNRETAPALSLESGTQLLPLSHFHAVLMRKDPPFDSMYLQATQI
                     LDYARGQTFLMNDPRGLRDANEKLYIFRFPDLIATTLVTRKMDEIKEFMAKLGGDIVV
                     KPIDGFGGSAVFHVRPGDSNVNAILEVSTHGEKRWVMAQRYLPEGKRGDKRIHLLGGE
                     PVGWLRRQPAGDDIRANLAAGGSAHATELTPRDREICARLKPSLVADGLHFVGIDVIG
                     DYLLEVNVTSPTGVQSVSRLYNFRLEGKIVDYIEAHAPRID"
     gene            complement(32160..>32532)
                     /locus_tag="PKL17_05480"
     CDS             complement(32160..>32532)
                     /locus_tag="PKL17_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44211.1"
                     /translation="FQSMAMVTLCKYGDPAWTQGQTYPGWRMLGNVRLDELVKYYPLV
                     LLLFGYFMVSSWRMPKFGKLKVRWLNTTMIVLLVVGYALATLQLAPEFLIGGGLLYMF
                     VATLAHFLWTPKERPEPFFPA"
BASE COUNT         6212 a         9663 c         9964 g         6693 t
ORIGIN      
        1 gcggacccac ctgcgacagc agatcaatgt catcacgcag tgtgtccaca tcgacatcga
       61 gttctttggc aagctcagcg acgggaacac cctggcgggc gagcaccgca ggaaccagat
      121 acaaaagtcg tttcagacga gcagcggtgt ccatggcggt tagctccggg cgacggcacg
      181 cggaaggctg gcttcacttg ggccggtgct gacgttgcgg cgagcgatgg cgcgggcggt
      241 ttcagcgacg cgctggcgga gtgattgggg cgcgagcacc cgcagcccgc ccgacgcaga
      301 cagcacgcgg cttatcaagt agtcggggtt gccgcagtcg aactcgacga tggtgccgct
      361 tgtgccgagg tcttcaccgg ggctgcgact ggcggggccg aagtcctcgt cggcgatcca
      421 gggggtgtcg gcgctgacaa acagcttggt gcggactttg gcttctcgct cgaaccgcca
      481 cggcgaaatg gcgccttgtt cttgcagtga aaagtccgcc ggaatgtcaa agtcaggcga
      541 cttgggacgc ggcgcgacgg tgagcttgag gatgcggtcg acacggaagc gacgaagacc
      601 ctgcgctttg tggcagtgtc cgacgagcag ccaggcacct tggcgataga ccatgccgta
      661 ggggtcgacc tcgcgcatgc tcgggccgac gatgctggat tcgctgccac cggtttcacc
      721 gtcggcgagc cagccgctgc cacggtgcgc gtcatattcg aacgtcagcc gcttgcggtt
      781 gtggatcgcg gtttcgatct gttccaggtg tgacgcgagc gatcccgact tgtacgtcgt
      841 cggcaagtgc accatcacat caagctgcgc cgggcctgcg gtttttgcgt cgccgccctg
      901 tcgtcgcagc cgcttgcggg ctggcagccc cgacagatcg aacatcagct tgcgcacggc
      961 cagctcggcg gcgtggcgat agggaaagtc gctttggtga cggacggctg ccgcggttgc
     1021 gacgagcact gcgacttcgt caggcgacag gaccacttcg ggcagccgat acttctggcg
     1081 atcgatgatg tagccacctt cactcggggc gtcctcgaca tcttcgagcg tcacatagcg
     1141 caccggaacg cccatttcga gcagcgtcgc tttgtctcgc tcgaatgcgc gtgcgccggc
     1201 ctcgggcttg gcactgcgat actcggcaaa ctgctcgcgc agctcggcaa acgaaatcgg
     1261 cctccgtgcg tcgaggagca gcactaccaa atcgagcagt cgcttggtcc ttgtatcagt
     1321 ggtggcgtgc atggctgcct cggaagtact gcgccgatgg gttggaagtc aagcgccgca
     1381 tcgcctagtg cgacgcccgc ggattggacg atgggctttg cgcaagcggc gcaagcgata
     1441 caaacggcaa cggcagttgg atggggcccg gcgaactttt ttcggcgacg ctggtttctg
     1501 ccgtggctgt gtcgctgctg tcgctgtccg ataagccgtg caaggacact ccgagcagcc
     1561 gaatcggtcg ccgtcccgcc tcagttttgt cgaccagcgc aatcgctcgc tgcgcaagct
     1621 gcacttcgtc tcgactggcc accgtcgatg tttcactgcg cgtgatggtt tgaaaatccc
     1681 cgtagcgaac ctttagggtc acggttttgg caaacatttg gcgacgggaa agccagtcgg
     1741 tgcactcttt gctgagcgcc cgcacttcat cggcaatccg ctctctgtcg atcaggtctt
     1801 gcggatacgt cgtctcactg ccgactgact tgcgctcgcg gtgaggctcg acggggcgat
     1861 cgtcttcgcc atgtgccaag tggatcagcg tgcgggcaaa gctgccgacg accaggctga
     1921 gatcggcttc gctgcgactc cgcacgtcga caagccgcgt cattcccgca tcgcgcagtt
     1981 ttttcgccgt caccggcccg acgccccaca gcgcatcaat gggcagaccc gacagaaatc
     2041 gctcgacgcg ctcgggggca atcaccgtca gaccatcagg tttgcgccag cccgacgcaa
     2101 ttttggccaa aaacttgttc ggtgccacac ctgccgacgc ggtaaggccc gtctggcaaa
     2161 cgatctgctt tttgatccgt cgcgccactt cgacgccgag cggttcattc caggcgtttt
     2221 ccgtcacatc cagatacgct tcatccagcg acaatccctc gacgagcggt gtgacggaac
     2281 ggaaaatttc gaacacctgt tccgacgcgg ctttgtagcg cgcaaaatcg ggcggcacga
     2341 cttttaactg cggacacagc cgcagtgccg tcgacatcgg aatcgccgag cgaacaccaa
     2401 accgccgcgc ttcgtagctg gcggctgcca ccacgccacg gtgcattcca ccgacggcaa
     2461 ctggctgccc gcgcagatcg ggacgatcgc gctgctcgac ggacgcaaaa aacgcatcca
     2521 tgtccacatg gaggatgcga cggaccggca tggctttgtc ggggattact tggcggcgtc
     2581 tttgcttgga cgcgcgcgca ttccacgggt gagacgggct ttgacctgga tgtgctgccg
     2641 ttctttgtac gcttcgtggc tcgattcgcc ttgctcgttt tggatcgcgg tctgcgcatc
     2701 catgacctga aattccgtca gcacataaac gaccgccgac ggatcaaact gcgcgtcatc
     2761 gagacccact tgtttcatga tcggcccgac gcgaagtggc aggtctgcga cgaagttaat
     2821 cacccggtac gacgccgacg aaaagaagtt cggctgcact tccggaatgt tgatctcggc
     2881 atcggactgc aaatcaggtc gcaagcgccg agtcgccggc tgcacatcga agattttttc
     2941 aaacggcaac agcgtgttta ccgactgacc ggggatcaca tagttaaacg ggacgagccg
     3001 gttgcatagc tcgcgcagga tcgctggcag gtcttcccag cgacgggtaa tcaagcgaaa
     3061 ccggatcttg tcgtagacgt gggccgcaag cgtatcgcgc ttggcgagca gcttggtgat
     3121 caggctgtcg cgctctttgc ggctccaggc aaattcgacg atcggcaggc ccgccgagcg
     3181 catttggtcg acgatctgca cgactttcga ttcgaccaaa ccgaacacct gatcgtcgga
     3241 aaccggcagc ttgaacagaa tttcgcgtcc gtcgaggtgc tgaagaacat gcatcacttt
     3301 cagcaccacg caggcatagg tctgatagcg tgcccgtcga gacgccagca gcagcagttc
     3361 atgcgccggg accccttcgg cgacttcttc ggggatgcga tagccaaagt ggcgagtcag
     3421 gtattcaaca gcctcggcac ggacgttttc gagccgttcc atgtcttcgg gactgtcggg
     3481 atcgaactca ttgacgcgta gaaagcgctg cacctgcgcc aaatcggaaa acgcgagctg
     3541 atgccagtcg agcaccgagc ttcccgcaag cagcagccgc accgattcga gatcggacac
     3601 cgttaccccg ctgccatcat cgacgacgga ttcacgccgg ctcgccggga ttttgcctga
     3661 cccagtctgc gaacctccac tttgcggcac gccggaagaa atgggagaag cggttcccga
     3721 aacgggctgc gcggctttgt gaagcgtctc gtcgttcatg ctcgtgtcct cgggtagggg
     3781 ccgaagcgaa gctcgctttg ttgtgctttc gtcctcactt tggtcctgca cgcgggtcgg
     3841 cttcttagcg cgagccggca caagacacaa gcgatgagcc tgctttcaaa gcttcgcagt
     3901 ggcaaagcgt ctgggtaaag cgcagttttc gtggtcgttt caaaccggcc aagaaagcgc
     3961 cgcgcgtcca tacagataga ttctagttcg ggggtctgat cacgcgaggg gatggggaaa
     4021 attgctgctc attttccgtt tgcgggcccg atcgttgtgc gcaatcagtc gtatttgccg
     4081 ctgctttcgg cgtctagctc ttccctcagc atcgcgagtg cgcgggcatg cagtcgagac
     4141 gtccacgact tcgacaggcc catgctggcg ccgacctgtt caagtgtctg atcgtggtag
     4201 tagtactgct cgatcaggaa gcgctctttg tcgggcaggc gcgccatcac ccgacggact
     4261 cgctgtgaca tctgctgccg ctcgatcatc tcgggggcgg gcgggcgctc gtcggggagc
     4321 tgggcttcgt ctttttgatc aagcagggtc acaaacaccg cggcgagccc accgagcgcg
     4381 ctggccagat cgcgaacccg ctcttcgacg ggatttgcgc catttgcggt gggctctcgt
     4441 tcggcctgtg actggatgaa gttgttggcg cgttcctcga accgcacccg ggcgtattcg
     4501 ctgcgtggca gccagcccat cgaccgcaga ccgtcgaaca tcgcgccgcg gacccggtag
     4561 tacgcgaacg tggtaaaggc gacgccatgg cggtcgtcgt atcgctgcgc agcctcgagg
     4621 agaccttgca tcccaaagcc gaccagatca tcgaactcga tcgccggcac ttgttcgcgc
     4681 agcttccgcg cgatcgagcg cacaaatggg atgtgatctt caaccagccg ccgcgggtct
     4741 ttcgctccgc tcttccctgg gagcgcggta ccttgcggct tcgactcggc catcgaaagc
     4801 agtatacccc gccgctcact tattgaggga gctcagtttc ggcgacggcg atttgcctag
     4861 cggcgcagcg gccaggttga aagcgccatc gcgaccagca ccaaaagcgc cccaacataa
     4921 ccgcgcagac cgagctgcgc ttctttcatc gccagatcaa cgacgagcgc gaacaccggc
     4981 tgcaccagtc caatcgtcga cagcactgat agatcgagcc gtcgcagaag cgccaaatag
     5041 acgctccacg cgatgacgct gccaaagatc gccaaaaacc cgagcgccaa gtgggccgac
     5101 aaaggcgccg atccaatgct cggaaaggtc tcaccacgca gcggcgccag caaaagcagg
     5161 ctcagtgcgg tgtagaaaag aaagtacagc gtcgaaatca gcggcgacag gtcgctcgcg
     5221 tagcgcttga gcagcgcgcc gtaaatcggc cacatcagcg ccgccgcgac gagcgccagc
     5281 agcatcgctt gaacttggct tgtaccgatc tgctgaccgt cccaaaacag cgccgcgatg
     5341 ccaaacaggc cgaccgacat cccggccacg cgacgcagca ccagctgttc cagcccaaaa
     5401 agccgcgcca ccagcagggt aaacagcggg cttgtggcac agagaatcgc cgtggtgccg
     5461 cccgacagct tcagctcggc caggtaaatc agcgcatagc caagaccgtt tacgacgccg
     5521 gctccgaaaa gcgccaggtg ttgccgcatg ccgccgggca acttgatcga ccgcttgcgc
     5581 gccgtgatga ccccgagcaa aaccgcgacg gcaatgaaaa atcgcaggct ggccccaaac
     5641 agcggtgggt atccggtcag acaaatgcgg atcgcttgcc aggtcgtaga ccagatcagg
     5701 cacagcagca ggtagccaag gatttccacg ctggccagaa ggttacgaat ttcgggggga
     5761 ggttggcaac gattcttggg attggtggag gtcgcgcgat cgtgcaacaa tcgccgccgc
     5821 tcgagacagt cgatgaacga aaaaaagtgc cgctgcggac acgacatatc gcacccgctc
     5881 gtcgtgcccg aaccgaaata ctctgcgtgg ggatggtttc ttctcatgat gggtgcgacg
     5941 ccaaagcctt tgcgtgcgct ctatcgctgc gagcgctgtc agcaagtcct gggctcgacc
     6001 cgcgacgaaa acgtcctgcg cgagttttcc taaagaaagc gtggcctgct gctgtgagga
     6061 cggcccgcgg tcagggcttt aggaaccgtc gctgcgctga cacaaaagga gtgccgtcgt
     6121 cccatgctgc gggtcttgcc atagctctgt tccgagcgtg acgaagccgc tttgggcaag
     6181 cacggtgcgc aagctcgaca gccgatagcg tcgctgccgg agcacggaaa actgtcgggg
     6241 caacatcccg cgggtttcga cggtggcgat gatgctggtg gtctggcagt cggggatcga
     6301 gtcgcttacg tcgagccgct gttcgagcac gatgtggtgc gggcgtggct tggcgtgcaa
     6361 aaacggttca aacagccagt ccgaaagcgc ggtgtcgtca accggcactt tgccgatcaa
     6421 gggctgcaga tcgctttcac ccggcaacaa aagatccgcg tccagcagca gcagatcgtc
     6481 tgggcgggtc actttggcga gcatatcgcg cagaaagcgc ggctcatgat ccaggtggcc
     6541 gagcgttcca agcaggacgt acatccgctg ctcatcgcgt tcgagcaggg gcgagatcag
     6601 atcggtgtat cgcggcaggt ggtgaaaatc gccgaggacg cccaatcggt gcacaagcgc
     6661 cggcccgagc atttccccag catgggcaaa cgccgtcgcc agcagcgctt cgcttcgatc
     6721 gacaagccac agcgagacgg cggctttttg cagcgcaata agcttttgca aaagccgaac
     6781 ttcaggctgt ccatcgccgc tgcccagcgc aatcacggtc agcccgcgcg tcgtcagcga
     6841 ccctggtatc ccgtcgacaa tttccgaaaa cggcagctgg gtccgtcgtt tttgctcacg
     6901 ttgtgagctg cggacaaagc gcagatagcc cgccgcggac tgcggggtca ggtacaggtt
     6961 gctctgctcg atttgccccc cggctccgcg cagtgtgcgc tcaagctcct cgagacagcg
     7021 aatcggctca tagtccggcg ccaggtaaca gttggccggc gtgctctgcg gcgaaaacaa
     7081 gcctttgggc agctctcgcc gaggcatcgg cagatcatga gacaagagcc caagccccgg
     7141 caccatcaga agctgaagca aggtccgtcg agacgggcgc gtcttgccca gctcgatgaa
     7201 cttgatggtt gcttcggaca gcccagacgc ttccgccagg ccgcggcgtg acagcccggc
     7261 ggtctcccga cggtcacgga ggagcttggc aaactccacg gcgctggtgg tcgtatcggt
     7321 cgaacgagtc gatggaacag cagtggtttt ggcagacgaa gtcatggcgg taagcgcgac
     7381 ggcggaagca gactgtgccg ctgcgtttcc gttattgctg tcgcgctcgc cgtttgaaaa
     7441 caggaaggag atgtcatcgt gtcgattctt gcagcgcgcg aagtgagtcc gccaaaaccg
     7501 caggccgcgt ggtcagtgaa tcagtccaag cttttttagc tgcgcggtca tctgcgcttt
     7561 gttgatgggc ttggtcacgt aggcatcgca gtgattgcca taggcatcgc tgatgttttg
     7621 gaaatcacgc agcgccgtgg tcatcacaat cttggcggtt ttcgacggtg gcagcccggc
     7681 tgattcttcg tgggcgcgaa tgctgcgcag cgcctgctgg ccgtctactt ccggcatcat
     7741 aatgtcaagg cagatcagat cgtaaggttc ctgtcgttcg agcgcgagcc ggacggcctc
     7801 gatggcttcc ttcccgttca ctgcgatatg cgagggtccg tattgcttca ggaattcctg
     7861 catgatcgtg cggctcacga aatcatcttc gacaattagc gttttcatcg tgttccccgc
     7921 cgtcgctacc cgatgggacg gttgcggctg cattgagtcc ggctgcttgg aacctggcgt
     7981 cctttggatt gtctgagtcg gcatgaaacg cgcagtgccc ccctgcaaat catactccag
     8041 cgcatctttt tcgcgtgccg ctcttctttt tacgctggtt tgtcttttgg ttttgctctc
     8101 cccctccgca atctcttcgt cgcccacccc ggagacatta caagggggac tgggtgtttt
     8161 cgtccgcggt agtcggctgc tgacaccggg aactgccgcc gcgctgcgca tcgcgaccca
     8221 cctggcgcct tctgaagcgg tggccaatcc ggtcggaaat gtcgccgtgt cagtgacgct
     8281 gtcaggtggt ggcaagcgtc agctcttgtc atcgggaacc acggatgctg gcggcggact
     8341 cgacgcaagg tttgtggttc cacagtggcc gcaaggcaag tatgaagtcg aagtccgcgc
     8401 cgagtttggc ccgcagatcg cagtcgaaaa acagaccgtc gatctggctg ctgccgcgcg
     8461 cattctgcta gagagcgaca aaccgcttta tcagccagga cagactgttc atatgcgggc
     8521 cttgacgatg cgttcgcagg atgggcatcc gctgaattcc ggaacgattc gttttgtcgt
     8581 caccgacccg cgcaaaaatc gcattttcca aagtgaaaaa ccgctgtcgg cgtttggcat
     8641 tgcatccacg gacttgccgc ttgcagacga gcttttgctc ggttcctatc acattcaggc
     8701 cgaacttgtg agccgtccgg atgtgtccgc agcagagcct gctgaacgaa atgtcgaagt
     8761 ctcgcgctat gttctgccga aaatccgggt ctctgtacgg gccgaacaga gctacacaga
     8821 gccgggcaag accgtgaaat tttccgtcgc tgcaaactat tttcatggca agccggtgag
     8881 cggcggtcag gtcacagcca aagcgtactt gaacgcggca tccgggcatc tggaactggc
     8941 cacactgcac ggcaagctgg atgaattggg ggcgctttca ttgtctgttc cggttcctgc
     9001 cgattccact tcagacaatg ccaagctgat catttcagcg acggtggaag atgctgcaag
     9061 tcagcgcgca gagagccgtc aagaactgct catcgccaag cagcccatca ttgtggatgt
     9121 cgttccggaa gctggacagc ttgttcccca ggtcgaaaat caagtctctg ttttggtggc
     9181 tcgccctgat ggcgctcccg tggaaaaagc ggctgtttca ctggtggtgg gcggcgctgg
     9241 cagcacgcca cttgcgggca tcacaagtgc aataggaatc gcccaatttt cgcttcctcg
     9301 tcttccggtg aacgctgctg gaacggatca aaaggggtgc cctgctgcgt cggtggctgt
     9361 caaagcgacg gtcacgattc cgggggcttc tgcggtcact ctatcgcagt gtctgtccgt
     9421 gcgcagcgaa ggcggactgt tgctgcgtag tgatcgcgcg gtctatgcaa gaggggacaa
     9481 gctgacgctg tctgtgcagt ctgccgcggt gcaagacgga ctgtgtttca ttgatgtggt
     9541 ccgtgacaag cagctccaag acacgctggt ggttccgctc cgaaactttc gcggccagat
     9601 tgcggttccg ctgcaagatc ggatggcagg aacggtctct ctgctcgcgt atgtggtggg
     9661 tccagatggt cgcaagatgc gcgatgctcg ccttgtttat gtcgaacgac cgtcggcgct
     9721 gcgggtcttg gcggaagctt cgtcgacaaa tggcccggct gggactccgc ttcatccggg
     9781 gcaaagtgcg cggctgcggc tgcgcgtcgt cgatgcggac agcggtgtcg gggtcaaagc
     9841 agcgctcgga ctggtgatgg tcgatgaagc actgctggcg ctgcgtccgc tgcggcctgg
     9901 tcttttgcgg gcgtactttt cgcttggaca agccgccaag cgtgctgccg cagaaagaca
     9961 gatcgcggct gggggcatgg gaatcgaagc gctcgtcgaa aaaggcggac tgtcggcact
    10021 ggaacaggat gccgccaagc tgctgcttgc tggcgcggtt gcaccgtggg atgacggttg
    10081 ggaaagcgat ccctgggcgg gcagggcgcg tgcgctcacc gaacagaaac gcaaatgggc
    10141 ggatgcactt tcgcactatg aaaagggcag aaccattggc gagcgggttc ccaatgagcc
    10201 agggaagtgg cgatatcgtc aggatctacc gtcgctgatt cacaaaagcg gattccttac
    10261 gtccgcgcag ctgctagacc cctggcgccg tccgctgcag accgatgtcc tgattgaagc
    10321 ggctgggttt cctgcgtttg acgaatacgc ccagcggctt ctggatgaac ggctgaccac
    10381 attttatcgc gtgctatgga acaagctgga ggcggatctg aagaccggaa aacgtcaaaa
    10441 ggaatccgat gggacggtga tattttccga cgcggacatc ggcaatctgc caggtgtggt
    10501 tctgcttgat ccatggggca cccagatgcg tgtacagacc cggaaaaagc cgcacaaaat
    10561 tggtcatctg ttgtccaaag tggtgctgta ttccgcaggt ccggacggcg tctttggcag
    10621 caaagacgat ctgtatccat cagacaatac gtgctatcac cacagctgcc ggctttccga
    10681 tcgcgacatc actgtcgtcg gagtaagcgc tgcacaggcg ttttcggatt cgaagcttgg
    10741 ctgtggctgt gggtatggtg cggcgggtgg tgcgctggct ggtttccatc gcggcacggt
    10801 cgcgtatgcc cggctgtcgg aggccgaggg cgctgcggtt ggtgcaagga aggactcggt
    10861 ccggagttcg tttccagaaa cgctgcttta tcgtccagaa gtgatcacaa atgaaaaggg
    10921 agaagcggaa gttccgctgg aactggctga ctcgatcacg acgtggcgac ttctggcgga
    10981 agctgtggcg caggatggac ggctggggag tcttgtgatg ggaattcctg tggtaaagga
    11041 cttcttcgtc gatctggatc ttcctccggt tgtgacacag cacgacgagc tggcgattcc
    11101 ggtcccgatg tataaccacc tcaaggtagg acagaatgtc gtggtgacgc tgcagcagga
    11161 gtcgtggttt gcaccgcttg gtccgctcac agatacggtg aacattcctg ctggacaggc
    11221 tgcggttcgc tatttccgga ttcgcgtctt gggagtcggc aaacacacgc tgcggctctt
    11281 tgcgcaaggc agtgccgcaa gcgatgcggt cgaacgtccg atcgaggttg ttcccgatgg
    11341 aatcgagcag gttgtcagtg tccaagaccg gctgtctgcg cagtctgttc agcacagcgt
    11401 acacgttccg gcgatgatga ttccaagcac cagcgaagcc gtcttgaagc tgtatccgtc
    11461 acccgcttcg catgtggtgg aaggactcga ttcgcttctg cggatgccgc atggttgctt
    11521 tgagcagaca tcatcgacca cgtatcccaa cgcgctgatc ttgcgctacc tgcgtcgcgc
    11581 tcgcaaaacg actccggcga ttgaacagaa agcgctggaa tatttgcaga cggggtatca
    11641 gaaactcctg tcgtttgaag ttgctggtgg tggtttttca tggtttggga gtgctcccgc
    11701 ccacaaaatc ctgaccgctt acggaatcga ggagtttgcg gacatggcgg aggtctttcc
    11761 ggtcgatgcc aaagtgatcg agcgaaccca gaagtggctg gcatcgcagc agcagtcaga
    11821 cggaagcttc gttcctgatc ggaacggaat ccgcgaaggc gcgataaacg ccgtcgtgga
    11881 tgacaagctg cgtaccactg cgtatatcgc gctggcgcta aaacgtaccg atcgcagtgg
    11941 cgtacatcgt cgtgtcgtcg atcatgcgcg tgagtttgcc gctggtgcac tccgtgatcc
    12001 aaaagtgctc gaacaagtga gtgaagaccc ctatacgctg gcgctgctta tcgatctgct
    12061 tgggccttct tacattcctc cttctgccaa gcgtcctgcg gagcgtccgc tctgggaaaa
    12121 gctgtgggcg ctgcgcaaac agaatgaaga cggacgcgcg gtatattttg cgtcggtgca
    12181 cgcgacgcct acctatggca gtgggaagtc aggagtcatc gaaaccacgg cgctggctgc
    12241 gtcgcagttt cacagcgcat ccttgccttc gcagagcagc agtgcgctcg cctatttgat
    12301 ctctgccaaa gattcctttg gtacgtggca ttcaacccaa gcgacgattc gttcgctcaa
    12361 ggcgctgctg gaacagcaaa gcggggatgg gaccgctacc agcggagtcc tcgatgtgta
    12421 ttgggatgga cagaagcact cgtcgcttac gctggtggcc aaagaagaag gtctgcggat
    12481 cgttgcgctg ccgccaccac caccgggcgc gcatcagctg tcacttcagt atgccggaaa
    12541 gggagtcttc gattaccagc tggtgacgcg ctattatgtg ccgcgcggtc aaagcgcatc
    12601 gccaccgcag ccggcatcaa atcgggcgat cggaatttcg atacagactt catcccttgc
    12661 gctgcgccgc aacgaaacgc tggtgcaaac tgtggtgttg gaatcgtcgc agtcgcacat
    12721 gatgccgctg gcccaagtgg gaattccgcc cggattttcc gtcgaaaaag agtctctgga
    12781 tcggctggtg acaagcggtg cgatcgaaaa gtatgaactt acgccgcgct acgtatcgct
    12841 gtatctgtcg gcactggatg ctggcgcacg caaggtgatt ccgattgcga tgaccgcgct
    12901 gcttcccggc aaagtccaga ttccggcagc atcggtgtat ccgtactatg aaccggaaac
    12961 gcgaagcgct gccgcgcctt ctgtcatcac cgtgcaagag tagtgtgggg gctctctgta
    13021 acaagctgcg ccccgcgcag aaatcagagc aggaagagat ccttttcgat caaatcggtg
    13081 atgctcgcgc gcaaatcgtg acgaccgccg acccagcgat acagcgcgct gacaatcggc
    13141 agggtcagtc gcgttcgctg cgagtgcatg tgtccttcgc ggatcatatt gagagcatcg
    13201 catgcgcgat cgatctgacg tgccgcttcc ccaagcgatg cgcctttgcc gatcagcttc
    13261 ccgagctgaa agtccggtgc gccgcgccgc tcggtggcga tgaccagctc gccaagtcct
    13321 gccggcccga gcatcgtctt ttcctgtccg ccaaacgccg cagcgagccg tcccatttcg
    13381 gccacgccgc gcgctatcag cagcgaacgt accgcggggc caagctccat cgcttcggct
    13441 acacccacgg caaatgcata cacaccgacc agggcgcgcg cgacttcgac accgatcaga
    13501 tcggacgata catagacccg gacttgcggc ccgtgcagcg cttggtacag catcgcgccc
    13561 acgtcgggac ttgcactgcc ggtgacgagc gcagcgggga cctcttcttc cagatcctcg
    13621 gcgagcgcag gaccggcaat cgcgccgatc tgggtgatcg gggtctcgcg cttgataagt
    13681 tcggacaccg gcagcatcgt ctgccgtccc gcaccctcga tcggggcaaa gctgccgatc
    13741 gcatgcacca tcgcttgatc gggccgcagt atgccgctta tggacttgag cagcggcttg
    13801 actttcgacg ctggcacggc caaaagcagc agatcggctt cgcgcagctc ggcaatgtcg
    13861 atcgaatctg cgtagacctc accagcggca aacatcagcg catcggcggt catttgcagc
    13921 gtttcgtcgc cgccgccacc cgatttcttg gccggaatca cgggcacccg catcgcgatc
    13981 tccgacggct cggcaaacgt cgccaggcgc ggcagatcgg gcggaggctg atagagcatg
    14041 gcgcggcgga tgcgagaccg ccactgcttc tggttgtgtt tcagacggac cgacagcgca
    14101 cgcgcccacc gtccattgcc aatcaccgcg atcgagaccg ctttcatctg gcattccccc
    14161 gaggcgactt gggtgtcgcc tcaaaaacca tgcgaccatt ataaaaaaag cggacccaag
    14221 cttctatccc ttgtcgcaca gcattgacat cgcgtcggac ccaaacgcaa gatgcgcgcc
    14281 aaacgacccg acggagaaat gccatgcgcc gctccttttc tgacagcttg tggtccgcgt
    14341 ttctgtgtct gcctttggtc gcaagctgcg gccaagatcc gaatccgggc gacaatcccc
    14401 cggtcgaaga gctgccgccc gatcccccga tctgctcggc tcctcccact gcgctgaccg
    14461 atgttggaac ttgcaaaggc ccgaccggag acccgctgcc ctgcgacgaa gcccatcgct
    14521 tctgcaagga aaagtactcg attcccaatg aagccggaat gacgcgggtc gagctgcgcg
    14581 gaaactaccg accgcttggc tgggatgtcg gttatccgct caagcagagc gcggacggaa
    14641 agacctggga aggggagctc tatcttccgc acggtcggga atcggaagtg aagtttgtcg
    14701 tcaacggcaa cgactggcgc gacgatccaa gtccgcttca tccgggcagc aaagtgaccg
    14761 tgcgctgtac gatgccgccg atcgcaacct gtgatcgcca gccgctcggt gatgctgaaa
    14821 gctgtgcaga gacgctgcca ccgacgggag acttcgactg gcgtgacacg gtgatgtact
    14881 ttacgtttgt cgatcgcttc tataatggga agcgcgaaaa cgacaagccg ctgcagaccg
    14941 acaaagcgga acgaccgggc gattatctgg gcggtgacta cgccggaatc atccgcaaaa
    15001 tcgatgatgg atacttcacc agcctcgggg tgaacgcgct gtgggtgacg gttccgtttg
    15061 acaatccgga ctacctaagc ccgataaatc ccaaagcgga caagcgtcaa gtgggcggtc
    15121 atgcgtattc cggatatcac ggctactggc cgatcgatca gtggccaaag agcggaaacc
    15181 gcgtcgaaga acacttcgga acggagtccg agctgaaaga actggtggcg cgcgcgcaca
    15241 gcaaaggaat caaagttctg ttcgattatg cgatggtgca tgtgcatgag cagagcccac
    15301 tttacaagtc gcacgctgcg gcgctcgatg gcaagggtct gccggttctg tctcccgatc
    15361 ggaagacgat cctcgacgga agcggcaagc cggcatggtt ctggccgaac atcgcgctgg
    15421 ctgaagacct gacccctatc ctaaaaaacg gaaatacgca ggagtgcatc tgtggatcgg
    15481 gtgcagagaa tccctgtcag tgggatggca ccaaagaaaa tccgctgatc ggcacgcgct
    15541 gcgcgttcca gggatatctg ccgcactgga actatgatgt cgatgatgcg ctaaactatt
    15601 cgctggatgc ggcggtgcgt ttgctgcgtg acaccgacgc cgatggctat cgtgccgatg
    15661 cggtcaaaca catcagcgcc aagtggtaca aaggtctgcg cgccagactg ctcagcgaat
    15721 tcccaaacaa gcgggtgttt gtgaccggcg agacgtatga ctttgggaat cgcgacaacc
    15781 taaagcgctt tgtcgattcc gcgacgatgc tggatggaca gtttgacttt ccgctgcgtg
    15841 cgaacttggc gtcggcgatt ctgtcgcgcg gcaacaccgg caagcacctg ccgatgtggg
    15901 agctcgcaga gtttgcgcgc accaacgaca cctattatgg ctgcggcagc atgacggctc
    15961 cgccgccggt gatgagcccg tggatcggaa atcacgatgt ggtgcgaccg attcacattg
    16021 ccgaaaacga tccgctgttt gatgagtggt cagatgggaa aaacagcgat ccggaaaagg
    16081 aacggacgtg gaccaatctg ccctcgcagc cgcagagtcc cgaagcctat gaacggctgg
    16141 ccaatgcgtt tgcattcctt ctgacgagtc ctggggctcc gcttatctat tacggcgacg
    16201 agtacgggct tgcgggtgga ggcgatccgg acaatcgccg cacgctgttt tatgcggacg
    16261 gaaccctgcg tggatcggtg cgaagctgtc atcagcttgc gctgtttgag cgggtcaaaa
    16321 aactgggcca gattcgcaaa tcccatccgg cgctgcgccg cggtgtccgc aagaccatcg
    16381 aagtcagcga cgatctgtgg gtctatgaaa tgtcgaccca gaaggcgtgg aaaacggagc
    16441 ccgcagaaaa agtctacgtt gcgatcaatc gcagtgacag cccacagact gcgtgttcgc
    16501 tgccgacggc tctgctgaaa gaactggtcg gagacagcat gatccaagga ccggcgattg
    16561 tgattccgcc gcgtcaggcg cgcatcttca tcgcgccgta atccgcagcg attcctcttc
    16621 cgcgcgctgc ggtcagtctg gtttgaccca ggctggccac ggcgccaaaa tccgtttttc
    16681 aaagtccgtc caaatccgcg gatcttcgcg gcagtcaaaa ccacgacagg cagcgggacg
    16741 cacttcgtac accgtgcagt gcttgctcgc gcggtcgatg tgcgtacagt atccatcgga
    16801 gttttgccga atcatgtacg gattgtcgat ctgccagcgc actttccctt catccagatc
    16861 ctgacgactc agcggaaagt gcagcgaaca gcagcgcgcc cgacagattg gcagcagact
    16921 gtcacaatcg acatcgctgc cttccatcac atacttgtct gcgaccactt gcagatgaac
    16981 ccgcggcggc tctccggcac tgcgtgcgac ccggcttagc agcttgcgat gatttggcgt
    17041 cagcgttccc tgcccgacga aaatgtcgac aagctgagac agctcggccc gcgtcgcagc
    17101 cagctcgctt tgcaggcgcg aaattgtctg gtcggtttcg tttagcgccg gtgccagtgc
    17161 gtcccacacc tcatccgcag accttttgga cttcccctca tcctctttgg acatggggcc
    17221 atcgtatcgc aagcgccaaa aaacgactac cgggcaagtg tcggcgtgca gccatcgccg
    17281 ccgcagacgt cgatgatttc acctgtgcgg aagaaattca ccatctgctg gctgtgggca
    17341 tccagccgcc gcggcttgct gtgcggatcg ccaaaggagt cctgtggatg ctgattccct
    17401 gccgctggcc acggattccc aaagttatac agaaccactc cggaaccgcg atacggatag
    17461 gcttgttccg tcgccagtgg cactttgcgc tgcttgtcgt agtttgccat cactttgatt
    17521 ccgatgtcgc tgcgcacggt gttttcgttt gtcaccactg cgacctgata gtcgccgcgc
    17581 gcgggagcca agagtacgta gtgcggcttg tttcctggga atggctcggc ggtgatgtgg
    17641 cgcaaatgac tgactggatc ggtgctgtcc cacagaagct gaatcgccgc gagcgcgaca
    17701 ttttggttta ccggatcggg ataggccgac gacagcaccg ggataaactg gttaaagtcc
    17761 gtcgaccgct ggagcagggt gttgtagttg ttgcccggca ctcccaagtg gccatacgtc
    17821 atgtccggcg tcaccgccat gaacgtgccg ccaaagattc cgccctgcga gatgcctgaa
    17881 tagaacagct cggacttgtt tacctggatc tttttgctct gcacaatcgg cagatccgca
    17941 agtcgctctc gcattgcgcg cgccagcagc acccactctg tcattccttg atgcagtcga
    18001 tcggtcagtg atcggaaacg cgacagctcg gacaggatcg acagaagcgg aatcgcatcg
    18061 tcttcacaca tgccgagcaa gttggtcgca aagaaaatca gcttgtgatc atttgcgatc
    18121 ttgccgttgt atccggcatc gacctcgtcc cccgcgttga ggagcccgtg tccatacatc
    18181 acgaggccgt gcggaactcc gcccagcgca ttgtgcggaa tgcgaaccca gaagtgcggc
    18241 ttgcgcgtcc cctgtgccct cggcagttct ttgccatcgc gatggatctg tgacccatcg
    18301 aagccgccaa tcttgacgtt ttccagatag tttggaactt caaacgaacc ttgcagctcc
    18361 agtccgatgt attcatcgac aggccgtccg gttccgtcat tggtcttggc atactctgtg
    18421 actttttcga cgacaagctc cggacctttg ccgcccacgt tggccagcgc atcatcgcgc
    18481 atggccaaaa gatcgctgtg catcgactcg ctgctcgcgg tgacaaactc ccatgccagc
    18541 gtcagagacg atcgcggaat tccttgtcct tccagaagct ggaacgtctt gtcaaagcgc
    18601 gcttgccgat cctttagtgc cgcgatgttc gcggtgtttc catcccgcag tcttgcaaag
    18661 gctggcgacg gtggaatcgt ctttccggcg cgatctttta tcgtgcgcag cgccaccaca
    18721 tagcgcgtgg cttctttcag aatttgtgcc ggacggacaa acagcgtcgg ctgcgaggat
    18781 tcctgatcgt actggtcgct ttcgatccag tagggaatgc gctttgcccc agccgctgtg
    18841 acttccaaaa gcaggagcgg cgcatcgctt gcgagcgact tttcaatcga actttccgac
    18901 gcaaatgcgc tcagatcgag acccggaatg gtcagcagaa tcggcgttcc tacgctgtat
    18961 ccatcacgct tgcgataggg ggccggatcg atggccacac tctgtttgtt ttccggaaga
    19021 gatcgattcc caaactggag agtgtagccg gtcttgcgcg tcgagtcctc tttcagatac
    19081 aggctggacg gccagggcag ggcgcacgct ggcggcagac ccccaatcgt tttggtatag
    19141 gcgtacccag gcaggatgac atcgcagtcc gcgtctccca agtacgcggt gaatggctcc
    19201 tcatccggcg gtgttggggt cgaaggggcg caagctgaca gcaaaaacga gagcgaaaac
    19261 agggtggtcc accggcggtt ccgtgagcga ttcgcgtgca tcatgacggc aacctagcac
    19321 ggcttttggc gcgcttcgca agtgcgtttt gcgcagcgcg gtggtgggct aacgcaaaag
    19381 caagttcagc agggggctcg attcatccgt ccacagaatc ggcggacggg ttccaatcgg
    19441 cgagctgtgc gggcgcagtg cagaaacgag cgcttcgctt ctgctcagga tcatccagtc
    19501 tgccgcatag atttcgtggg gatcgtcctg cggtgatcgc accctcgtca cagtgaatcg
    19561 tcccttttcg gcaagtccgc gcaccaccgg ggccaagtcg aggtagcgat tggtgatgtg
    19621 aatcacgagc gctccatctg gtttcaggtg tcgctggtac gtcgcaagcg cttcttgcgt
    19681 caaaagatgg gtcggaatcg cgtctccact gaacgcatcc agaaccagca gatcaaacat
    19741 ctgttcggtt tcctgggcga gcgacagtcg tgcatccccc agcacgacgc tgactgtgcc
    19801 ggaacaggaa gacagatagg tgaagtacgt ttgcgccagc ttttcgacga cgggatttag
    19861 ttcatagaaa cggaaacgcc agcctggacg tgcgtaggtc gccaaggttc cgatccccag
    19921 tccgacgatt cctgcgtgca ggtgttcccg attctcaagc tgcgcaaaca cctgaccgat
    19981 gccgcttgct tcatcatagt acgtgatcgg atcgcggcgg aacttggcgg actggtactg
    20041 gacgccgtga atggttcctc catggaacat cgcgcggtcg tgttcatccg gtcggttcgg
    20101 aacgcgatca tggacttgta cttcgccgta aaagttgcga acagatgcca agatcgtcgt
    20161 gtccggatgt cgtctgtgct gatgctgtag cgcaagcaga attcccaccg tacccagcgc
    20221 ggcaggaaac agcgtgcgga tgcgtgcagt ccagtcctgc tcatcgatca cacgcacgaa
    20281 aatcgcgatg agatagcagc cgagcagcgt tccgtaccac tcggcggtgt gcgacaaaag
    20341 aagcggtgac agaacagcca gaagcacgcc gccgagcact ccgcccagcg agatgcgcag
    20401 atagtagccg gtgaggtgtt ctgcccctgg cctggtctgc gccacttccc cgtgacagat
    20461 catgcaggtc gaaaacagca gcacaagcga tagaaagaga ctccgtacat aggcgtactg
    20521 acccagcact ggacccagca gcgagggaaa actttcggac acaagcagca gcgccaccgc
    20581 caaaagcgtc agcaccgcaa accgctttcg acggtaaaac cgttcacttt caaacgtcag
    20641 gatgaatgac agaagataga ttgcgagcgg cagaacccac agaaacggaa tcaccgcaac
    20701 gtcttcacac aggtgattgg tggtggacag aagcatcccg cacgccagca ttggcagcag
    20761 gatagacatc gcggtctctg tcttgttttg cgtccgctcg gcatcggctt gtggcggtag
    20821 atctggcgac gatgatgtgg gcagcgcagc ttgcaaggct ggtttgagca gtccttggta
    20881 cgcgcagagc agccacagtg gcaaaaggag tccgaatccc agcgaccaca gcttggcctg
    20941 cgtcgtgacc ggaaagagtg gttccatcac cagcgggtat ccgaaaagcg ccagcagcga
    21001 tcccgcattg gacagagcat agagccgata gggagaacct ccgcgaacgg acagccacgc
    21061 ttggctgagt ggccccgtcg ctgcgagcag aaagtaagga acccccacag tccgcacaag
    21121 cagcagcagg atgcgtggaa tcggcgcatc acttccgtgg ggccgcagcg cgtcggacgg
    21181 aatgatcggc agaaacccca gcgacagaac caccagcgcg acatacagcg aaaaaaccca
    21241 gcgtccgagt ccacgctgca aaagccgcgt gcaaatgtga gcgtacagat aaccgcccag
    21301 cagcagcgct tggaacagca gcatcgccgt ggtccacact cccggcgttc caccaaacca
    21361 aggcagaatg aacttcccga gcagtggctg cacaagaaac agcagcagcg cgctcacaaa
    21421 acaggtcagc gcaaacggca gcgtgtgttg tctattctgc gcagcagggg atgacgtatc
    21481 gcgatccacc ccctattcct atcacgtcac tatccgtggc cttgctgttt cgtcggatct
    21541 tcgggctgct ggtgtggcat gtgctgactg tctatcactg tgtacttgta gcgatgatcg
    21601 gggtcggcct tcatttgatg gatgaagttc tgccatgggt cgccgcccca gccgccgcga
    21661 atgacctgac agccggcaga ccagttgctg acttgcggtt caacgccgcc agcgtggacg
    21721 ttgattccga accagccact ttccgacttt tcatttttct ggcggattcc gtctttgttg
    21781 gcgtcacgcc agacgttgac tgcttcgcct tggtttagcg ccgtgtgacc tttgtggtcg
    21841 ccgagcacgt agtcgtactg accttccatc aagttggcat cacccgccgg attgtagtac
    21901 ttcgggaatg cccctggatc gacggtcgcg ttgtactttt ctacgtggtg cttgcccagc
    21961 ttgtcattcc acagcaccgc gagcgtgtcg ttgtactgat ccttgacatt gggaacgggg
    22021 tgtccgttca tccagccgcg cacaccgacg atgttgcgct gctcgggccg cgcgtcaaac
    22081 tgacctccca ttccttccac ggcgtggcga tagtattcgt acttcgcggc ttccgtttgc
    22141 ggttctgcca ccggccccgg ctgcgcatgg gcttgtggct tttcggttgg ggtgggaacc
    22201 ggggcatgag ccggaacggg cactttgctg ggggcttgtg cgggggcctc cgtagccggt
    22261 ttggcaccgt cgggtttcgg ggcctgcgtc ttgtcttcta gccacgggtg tgccgccatg
    22321 gtcagggata tgttgctgcg tggatctccg gcttccgggg ccggcttggg cgcttgcgtc
    22381 ttgtgctcgt gttcgcgcag cgcttcgatc atcgcgactg taccaagctg tgagtctttc
    22441 gcgctggggt cgtagtgacc gtcggcaaca tacttcccac gctggtactg attggttccc
    22501 gaaaacgcat acggactggg cttgtctttg tagtggtatc ccaggccgtt gtaatactcc
    22561 gaatagctcg cgagcgctgc catgtcggtg gtgtcgcggc caatatgaaa catttgctgc
    22621 gcatcttttt gccgatgcaa ggcatcgctg gccgcatctt cccacttgta gaacgtcgga
    22681 atgttcgtcg gccagtgccg tggcggtcgt cccagcggat caccctgatg caaatacgtt
    22741 ccgaagtctc ctgatgactc tcgccagtgc agcgcggcga tgagctcggg cggcatgttg
    22801 gctttttgcg ccacgctgtc atagcgcgct tcgttcttgt cgaagttgcg caggaacaga
    22861 tccatgtcgc ctttctgtcc ctggctaagc tgcagcttca ggtcgcgcag ccgcgctttg
    22921 ttgtcgtcgt acaccgcacg tccttccttg tcagaaggga cgtgattcgc gttgtcgggc
    22981 ccagtgctcg cgctgtcttc gtgctgaagt tccttgatcg ccccgacggt cagcggacca
    23041 actttgccgt ctggacccca gcctttgctg cgctggagct ctttgaccac ggcagaagtc
    23101 ttgggatcgt acacacccga cagatccact ttgtatcccg ccgcgacaag ctgcctttgc
    23161 agcattgtga ccgatggtcc ttgctcgccc ggttcgagga tgtcttttcc gctggcgact
    23221 cctttccagc ccactacttc ttccagtccg ttgctggtct gggggcccag ctcgccggtt
    23281 gcctggatgt gataggtgtt ctggaacttc agaagcgctt ttttggtcgc ttcatcgaac
    23341 acgccggacg ccggtactgc aaagcccgct tggcttagct cggactgcat cttggcaacg
    23401 gctgcaccac tgtctcctgc cttgagccgc gtttggcctt tgagcacgct gcgccatgga
    23461 tcggcgtttt tatcgctgcc agctttgtcc gcttgggcct cgctgctggg cgctttgggg
    23521 ctgctcgccg attcgtgacc tggtgtcact tccgcgtgtg cggccacgcc atctgttttg
    23581 acggggggcg caggctcggg ctcctgggct ttgggcggtt ctgccttggc gccggctgct
    23641 ttggccgctg ccaaagcagg tgcaggatga ggagtcgctt cgctcccatg gtgtcctgca
    23701 tcgctcgtac cgccgaggtg gctcagaaaa tcgcccgcct tgtgcagcca cccatcaatc
    23761 ttggcacccc agccttgcag ggctttgacc gcgcctgggt gtgccgacaa gtacgatgca
    23821 acgccttgcg ccgcaatcgg cgcgaccgca tcccacatgc tgtgcggctt gcctgattcg
    23881 atctgcggct gtgcagccgg atgctgcgtg gttgccgtgc cgtcggtggt gtgggccggc
    23941 ttggcaacgg cgtgaaaggg ggacggttcc ggatcgtggt gtagctgctt gggcatctat
    24001 ttttcattca tcggcagcac ggccctttct gttgccgctt gatatccctg cgaaatggct
    24061 ggcctaacgg gtggtccatc tcgctacacc cgtgccgctg cggcgccgct tgggggacag
    24121 gaggggattg caatggcgac ggaacttgcg caccctagag caaccgacga gatgtcaagt
    24181 tctcagcctc cgcgcgctct gtgggcgcat ccgctccagc tggtcgatga cctgtcgctt
    24241 cctgcggcgc ggcttcggct tggctatctg ctgctgatgc tgggaatgct gccggtgctg
    24301 ctgctgctcg ggacggcgcg ctttccgtcg ctcgccaggc tggcggcgtg ggtcgggctg
    24361 ctgctttctg ggcttccggc gctccacatc gtgaccagca agcgggcccg acccagcgcg
    24421 gtggccggtc tgtcgctgat gattgcgctg tgttacttcc tggccacgct gcatgaggag
    24481 cggctgcttt tgcgctgggg cgaagcgcgg atcacaggtg gggccgtcac gttggcgatg
    24541 gcctatgcgg cggtggcgat tccggtgctg tcgctcggga cgtggcttgg cggcgcgctc
    24601 ggagtcggtc gggtatttcc caagctgcgg ctgccgctgc cggaacgcgc gcttttgtgg
    24661 tcgggcgtgc tgattgtgtc gtggtcgctg ctgcttgatg tgctgcggat gcgcgggctg
    24721 gttccccgtt cgcagcagct tatcagcgtg ctgtcggcgc tggccccgat tgatctgggc
    24781 tatgcgctgg tacttttgcc cagtcttcgt ccgggtcgcg agcgattggg ctggctgttt
    24841 tggggcctga cggtgctgtc atcgctggtc gcgctgatga gtggctcgct tgccccgatg
    24901 gtgcagccgc ttttgaccta tctgctcggc tggctcttga tccggcgtcg cctgcgcgtg
    24961 tggcccgttg tgctcgggac gctggcggtg ctgctgttcc agccggtcaa gggcgagttc
    25021 cgcgccaagg tctgggatcg cgaagtgcgg ctgtcgctgt ttgagcgggc ccagctgttt
    25081 gtcgatctgt cagcgcggca ctggctgggc ggcgagaccg acaagaccgt cgaccgcgag
    25141 cgatccgtca aagtggcggc ggcgcgcacc gcgtcagccc tacagctgtc gcacatcgtc
    25201 gaaatgaccc cggcggcgat tccgtaccag aacggcgcga cctatcggta ctttcgctat
    25261 gcactcttgc cgcgggtgtt tttccccgac aagcccagcg cgcagtacgc cgatgtgtgg
    25321 gccgcggtga tgtatggcta cacgacaccg agcggcaccc aacatgtgat ggtcggtttg
    25381 ccccagcttg ccgaggcgta tctaaacttc ggcgtctttg gcgggcttgg ggtcgtgctt
    25441 ctgtgcggac tcttgctgcg catcgccgac gaaatgttgg cgcaccgtga tgccggctgc
    25501 ggtgccgtcg cgctgtatct gttttttgcg cagcaggtga tgcggacgtt cgagggctcg
    25561 gcgggtcagt tctggggcgg tgtgcttcag cagttcggtc tgtatgcgct gcttctgtgg
    25621 gcttatggcg cgctgctccg cgccgcatca aaccgtccgc tgtcgagcgt tccgagcagc
    25681 gtatgaacct tggcttgggt gatgaggacc ggaaccaggg tgccggtcag tgcatcgccg
    25741 ctttggcctt cgggtagctc gacattgacg gtgtgattgc caccggtgcg tccgcacaac
    25801 tgactcggat tggcgcgtgc acggccttcg atcaggatct cttcggtccg tcccaccagg
    25861 cgctggagcc gcttgcccgt gatctgtttc tgcaagcttt gcagcgcagt caggcgacgg
    25921 gacttttcct ctgcgggaac atcatcggga agctcgaccg cgggggttcc ggggcgacgt
    25981 gagtaggcaa aggaatagat cgaatcgtat tccacttccg aaagcagcga catcgtctga
    26041 tcgaagtctg cttccgtttc gcccggatat ccgacgatga tgtccgtcga caaagagatg
    26101 tcgggacaga cctcgcgcag ataggcaatc ttttccagat acgcttcgcg cgtatattcg
    26161 cgcgccatcg cggcaagcac atttgtcgac cccgcttgca caggcaaatg cagccatggc
    26221 ttgagcgtgc gcagatcccg gaatgcatcg gcgactttgc gcgtgaagta gtgcgggtgg
    26281 ctcgtggtaa agcgcagccg caaaagtcct ggcactgcat cgacacggcg gagcagctcg
    26341 gcgaagtctt caccatctgg ggttccgatc gcgtgatagg cattcacgtt ctgcccaatc
    26401 agtgtgactt cgcgcgcccc cagctcgaca aaccgcgtga cttctttgac cacttggtca
    26461 gccggtcgcg acacttcccg tccgcgcgtc cgcggcacga tgcagtacgc acagaagttg
    26521 tcgcagcctt tctggatcgt caccagcgcc gtcactttca catccgaggg gcgcgggtcg
    26581 gcatccagaa actggtagtc ttcgacatca atgacttcgg tctgcgcgag ccgcagcttg
    26641 tagcgaccgg ctttgggcgc acttttgggc cagctttcca gctgcggacg cagcgcgcga
    26701 aagtgctggc ccaggctcgg aatctggtcg ggaccaaacg tcaagtccgc gctgggaatc
    26761 cgcttcagca gcgcgtcgcc ttcttgctgc gcgacacaac cgccgatggc gacgagcagg
    26821 ttcggattgc gctctttgag cttctgatag cgcccggctg ccgatgcggc cttgttttct
    26881 gccttttcgc ggatcgagca ggtgttgact acgatcatgt cggcttcttc gaccacatcg
    26941 gtccgctcga aaccgtcgcg tcgcaacacc tccgtcatgc gctcgacgtc gtagtcattc
    27001 atttggcaac caaacgactc gatgaatacg cggcgtgcgc gagcggcacc cagacccgtt
    27061 tcactcgcat ggtgcggcaa aggttgcgca gaggaagcag aatgggaatc agacagaccg
    27121 ttcacgtcgc gcactttccc accctttgtt tcccaaggtc aagcagtcgc gtgccttcgc
    27181 cagccggctt agctcgctat tcgacaggtt ttgtcacagt cagcccgatt gacagcgggg
    27241 ccggggccaa aatacggtcc cgcccatgac gcgacgactg ggctttacgc tgttctccgc
    27301 gctgctctgc atcggaaatg ccgcacatgc cgacaagccg gtacgaacct ggaactacct
    27361 gacgaccggc aacggtcacg gcttccagtt cttcgacacc aacaaaaaca agatcacgca
    27421 ctttctcgaa gcgccgtatc gctatttgcg accgcgatcc gacccgcgat ccgacggtta
    27481 tggccggcgc aacctcgctt atgacttcta ttttggcgta aagagtggat cgggcggcgg
    27541 ctggctcaac accggcacgg cgggggacgc cagctacctg gaagaaacca acatcatcaa
    27601 agtcccgatc acgctgtcgg gagtaagcgc agagacctat tactttgcgc cgtttggttt
    27661 tgagggcaac gcgatgatcg ccgtcctcaa agcaccgggc gcaaccgatg gcttcgcgct
    27721 gtttaacttc cacctgggcg acggcacacc cgacacgccc aatgccaaca gcgagcggtt
    27781 aaaagtcctg tcggacggca agtcgattgc cgaaagcggc cccggtggcg gcgcgatgat
    27841 ctatgtgccg atcaccgcgc tcgcgcatgc cgactgcgat agtgcgtgga gcaaagtaaa
    27901 cagcggccag tcgcttggcg acaagagcaa ctgtagtggc aacgatgtcg tcccggcgtt
    27961 tcagtccagc ctcgacggtg ggtatatggc ggtggcgacg ctgtttgccg aaaacgagtc
    28021 cgatgccgag acgctgctcg cccagtttaa gacctggtta aatggccgcg gcgctcagaa
    28081 agtgctgacc gacgcgctcg ccgagtggac cgcatggcgc aagccgctgc cgactggact
    28141 tgcgctgtgc accgacgatg aaaagaagct gtggcggcaa agtgaagcgg tcctgcgcat
    28201 ggggcaagtt cggcagccga acacctcgtc gcgcaaaaac ttcggcatga tgctcgcgag
    28261 tctgccgccg ggtgagtggc acaccggctg ggtacgcgat gggctttatg ccaccgtcgc
    28321 gctggcgcgg atgggacatc ataccgaagc caagatgggg ctcgactttc tgttaaatgc
    28381 cgagccggtc agcaagtatt cgagctatgt ctcgggcgca agctaccgga tctctgtctg
    28441 ccgttacttc ggcaccggag aggaagaggc cgactattcc ggccaacaga cgcccaacgt
    28501 cgagatcgac ggctggggca tgaccctctg ggcagcgcgg cagtacgttg aagcatccgg
    28561 cgacacagcc tggctgtctg aaaaaacacg gcttggcccg tcggtctacg atgcgctcta
    28621 cagcggcgtc gccgaagcgc tgcgcaagaa caccgagtca tccggcatcg ccaaagccga
    28681 ttcctcgatt tgggaagtcc acgacgaaaa caaaaagcac tttgcctaca ccaccctggc
    28741 gaccattcgc ggcttctgtg attttgccca gcttgcgacc aaggcaggga agggcagcga
    28801 cgcgacgacc taccgcggtc tggccagcaa agcacgcgat ggtttcttgc agtcgtttgt
    28861 cgaccgcgaa ggcgcgattg ccgggtcggt ggaagagctg gcgcagaacc aatatctcga
    28921 cggtgcggtg gtcgaagcct ttacttggaa cgtgctcaaa gattccgaat acgtgggtcg
    28981 caccggcaaa gccacgctcg atgtcctggg ccggctgcgc gtcgaatccg gcggctttaa
    29041 gcgcaacgac gacggcaagt cgtcctacga caacaacgag tggatcatga tcgacctgcg
    29101 catggccgac gcgctgtggc gggcaggtcg cgagagcgaa tccgcgggtc ttacccagat
    29161 gatcatcgac aaggccagcg cgaactacta cctgttgccc gagctttaca acgcggtgcg
    29221 cgctgacgga gccgtcggca agtacatcgg cagcattccc atggtcggct acggcggtgg
    29281 ggcctatgtt ctgacgatgc ttgatcgctc gggacttatc gaacccaacg actgcggcga
    29341 cggcatgggc aacaaaagct cggggcgggc gctgaagtgt tcggatccaa aggtgacacc
    29401 gaccaatccg acgaatccct ctgcaccgac ggcagatgag cttccctacg aaaacgcttg
    29461 tctgtgtggc atcggccccc gtcaccgccg tgtgagtccg cttgtgcttt ctgccgggct
    29521 tgttctgggt acgctgctgt ggcgtcgtcg tcgcgcgcgg ggagtcaagg catgatgtcg
    29581 cggtcgctgc gccctgcgct gtttgcgttc agtttgttat cagccgtccc atcggtgtgg
    29641 gcagatgagc ccgccgatgc ggtggctgcg gctgccccag cccctgtggc gataccccaa
    29701 gtcgaagcgg aaccgccagc caagtcgctg cctgttccgg ctgtccctgc ggcagcggac
    29761 gcgcgaccgg gcatgggcgt gacgattggt cctgtggaac tcaaagcgac cggctatctg
    29821 cgtgcgccgc tccgtctgtc ctttcgcagc cgtggcgatt ccgtcaagga aggcgaaggt
    29881 cagtacaaca tccatacgcc gtggcttgtg gatgacgact acttccggtc gggctttcag
    29941 tacctgcgca cccaggaatc cgactggtcc gagctgtatt tttccgcgca gagcaagtac
    30001 ctgacggcgg atgtcgcgct catggggtct ttgtttaccg actgggcgcg tcctttgctc
    30061 gaccgccagt ggggcattgc gcaaggctcg gtgacgtttc gctacgaagc actggggcca
    30121 aagctcaagt ttcgcatgca cgtccgcgcc ggttcgttct gggaccgctt tggctggctc
    30181 gaaaactacg acacgtatgt atttggccgt acccaccaga tgggcggaca ggttcgcttc
    30241 gagttcgaga ccaggcccgt caccttctgg cttctgcaag gcgtcggcgc gcacctcgac
    30301 gccattgagg caaaccaagg tctgacgctg atgaactacc tgcacctggg ggccagcgtc
    30361 tacaaagttg cgcacctggg cttttacttt atcgacacgc tggcccagga caaacggcag
    30421 ctcaaagaag tgcaggatgc gtcgatgcgc gtggtcggtc tcgacctgcg gctgaacccc
    30481 tttttcggca acttctatct ggccggatcg atcatcacgt cgacccaggc gcagtacatg
    30541 agcccggtgc tcgaagtcat gcactcgtgg ggggggcgcg gctttactga aaactatctt
    30601 ggcaccgaca agagcgacgg caccggatcg ctgaggagtc tggccggcga atacaccttc
    30661 agcttgcggc gcgtgctgtc tgcggtggcg ccggaaaaag ccggcttttt gcgcggcggc
    30721 gatcttcagt ggaagtggtt tggcctggtc gcatacaccc tgtccaagca ggtcgatccc
    30781 gacccatcca tcaaccggga cgggcggctg tcgttcaagt ggggcaccga gctgttttac
    30841 cagccgatgg cgtttgcctt tgtggcactg cgctatgacc gcgtgattct ggatgtgcag
    30901 gacgatgcgt cggccatccg cgtcatctca ccgcggatcg gggcgacggt gaactggctg
    30961 ctgggggcgc agatattcct gcagtactca cgctatttct atggcgaacg ggtccggctg
    31021 cgcccaggtc aggtggcgct cgagaccttg cctgacgccg acgtggtcaa gctgcaagcc
    31081 caagtctcgt tttagcccgc gctgtgccgc gcttcgcccg ttcccccttc cccaaaaagc
    31141 agtgatagag tttgggccat gcgcgtcctc gtcatcatcg atccgcctgc cagtcttcgt
    31201 ccgcgctctg atacatccgt ggccctgatg gatgaatgct tgcagcgggg ccatcaggtc
    31261 tttgtctgcg agcttcacga tctgtcgctg cgggactgca agccctacgc gctttcgcag
    31321 cgggtgacgg ggacgaaccg ggaaaccgcg ccggcgcttt cgctggaatc agggacgcag
    31381 ctgctgccgc tttcgcactt tcacgcggtg ctgatgcgca aagacccgcc gtttgactcg
    31441 atgtatctgc aagcgacgca gattctggac tacgcccgtg gtcagacgtt tctgatgaac
    31501 gacccgcgcg gactgcgtga cgccaacgaa aagctctaca tcttccgctt cccggacctc
    31561 atcgcgacga cgctggtgac gcgcaagatg gacgagatca aagagtttat ggcaaagctc
    31621 ggcggcgaca tcgtcgtaaa gcccatcgac ggctttggtg gctctgcggt gttccacgtc
    31681 cgtcccggcg acagcaatgt caacgccatc ctcgaggtat cgacccatgg cgaaaagcgc
    31741 tgggtgatgg cgcagcgcta cctgcccgaa ggcaagcgcg gcgacaagcg catccatctg
    31801 ctcggcggtg agccggtcgg ctggctgcgt cggcaaccag cgggcgacga cattcgcgcc
    31861 aacctagctg ccggcggttc ggcccacgcc accgaactga ccccgcgtga tcgcgaaatc
    31921 tgcgcccggc tcaagccctc gctggttgcc gatggacttc actttgtcgg catcgacgtc
    31981 atcggcgact acctgctcga agtaaacgtc acctcgccga ccggggttca gtcggtcagc
    32041 cggctctaca acttccggtt ggaaggcaag atcgtcgact acatcgaagc ccacgccccc
    32101 cgcatcgact gatcgcccga tcggcaggcc ggggatgtgg tgaatcggtc tgacggacgc
    32161 taggcgggga agaacggctc ggggcgctct ttgggggtcc acaggaagtg ggcgagcgtt
    32221 gcgacgaaca tgtacaggag tcctccgccg atcagaaact cgggggccag ctggagcgtc
    32281 gccagcgcgt agccgaccac gagcaggaca atcattgtcg tgttcagcca gcggaccttg
    32341 agcttgccga acttcggcat gcgccagctc gacaccatga agtagccaaa caagagcagc
    32401 accagcgggt agtacttcac caactcatcg agcctgacgt ttccgagcat ccgccagcct
    32461 gggtaggttt gaccttgggt ccaggccggg tcgccgtact tgcacagcgt caccatcgcc
    32521 atggactgaa aa
//
LOCUS       DAORGN010000030        32379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_510_length_32379_cov_5.839717, whole genome shotgun sequence.
ACCESSION   DAORGN010000030 DAORGN010000000
VERSION     DAORGN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 32379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32379
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_510_length_32379_cov_5.839717"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1478
                     /locus_tag="PKL17_05485"
     CDS             327..1478
                     /locus_tag="PKL17_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNK44212.1"
                     /translation="MTQPALPPGAIVAEKAPPPFSLLDYEGNLRADADPSLVPDISDA
                     DCLRLYREFIKLRLVDDRYLKLQRQGRLGFYMQSTGEEATQLGVAYALRQTDWIYPSY
                     REPGIAFFRGYSIHDFTNQLFGNAEDPIKGRQMPVHHSFRAGNFVSISSPVGTQIPQA
                     VGTARAAQILKHDTVTSVFFGEGTTSPGDFHVGLNFAGVWKAPVIFVCRNNGWAISTP
                     ISVQTASPTIAHKALGYGFPGFLVDGNDILAMISATRIAAARARAGEGPTLIEALTYR
                     RAAHSSSDDPSVYRKGKDDEIKEWERRDPVARFARYLERRGILTPAIDAQYRDEITQE
                     ISAALAHIEHVGTPPLMSLIEDVYAQPTAPLREQLAELLTLPRQKLGHG"
     gene            1509..2483
                     /locus_tag="PKL17_05490"
     CDS             1509..2483
                     /locus_tag="PKL17_05490"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNK44213.1"
                     /translation="MPVYNIIQAVNDALRIEMRKNSNIVVLGEDVGNFGGVFRATSGL
                     QEEFGKDRVMDTPLAEAGIIGAAIGMSLYGLRPVAEIQFADFIFPAFDQIVNELAKFR
                     YRSGGEYPAPMVIRTPYGGGIRGGHYHSQSPEAYFAHTPGLKIVIPSNPYDAKGLLLQ
                     AMREEDPILFMEPKRVYRASRGEVPEGEYTIPLGQAKVVRPGKGVTVLCYGSMVHTVM
                     EAVEKQNAAEFDPEVIDLRTIIPFDAETILDSVKKTGRVVIVYEAPKTCGFGAELAAF
                     IAEKAILHLEAPIARCAGLDTPFPYTLELEYLPSPERVAKAIADTARY"
     gene            2487..3803
                     /locus_tag="PKL17_05495"
     CDS             2487..3803
                     /locus_tag="PKL17_05495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNK44214.1"
                     /translation="MALEFKLPDIGEGVVEGEIVRWLVKEGDVLREDQPMVEVMTDKA
                     TVEIPTPRAGRVHKLMVDAGKLCAVGQVMIVIDTGGTASPSAAPTPAAQAPVTQAAPA
                     QAAQAQVSAPVQTVSAAEAAGRKVLATPATRQLARNLGIDIRTVLGSGPGGRITRDDV
                     RRLSEKATAPAQAAPIPVQAPAAAQKPAAPAPRPAAEPVAVAVSADDERIAFRGVRKK
                     IAENMLRSKQSAAHFTYVEEVDVTDLVALRKRAQKRAQERGAKLSFLPFIIKAVVSGL
                     KKYPILNATLDQQREEIVLRKQYNIGVAAATDSGLIVPVLRNADKMSLFQIASELEPL
                     AERARTGKASRDELTGSTFTISSLGKLGGVLATPIINFPEVAILGVHKIKETPAVRDG
                     QIVVRQVMNLSISLDHRIVDGYEGAMFLQHVVSLLEDPTLMFMELT"
     gene            3807..4943
                     /locus_tag="PKL17_05500"
     CDS             3807..4943
                     /locus_tag="PKL17_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNK44215.1"
                     /translation="MRNAPPSFGFAPPAEQFVPRQYLAPNGSFDPTQVAALGIPRDQL
                     LKLYRGMLLIRIIDERMLLLQRQGRISFYGEARGQEAAVVGTAAGLGPQDYILPALRE
                     AGAALYRGLPLRTYISQIYGNENDVTKGRQMPCHPGTRQARYVTMSSCIATQLPHAVG
                     MAMAAKIRRDDVVIAGYMGDGATSEGDFHVALNFAAVYKAPVVFVCQNNQWAISTPVA
                     GQTASPTMAHKALAYGIPALRVDGNDVLACYTAMRDAVAYARSGGGPMFVEALTYRVS
                     AHSTSDDPSRYRDESVTAVWKTERDPIQRYRHFLRVIGALTDGVDEALHEAIEAEIKA
                     AVLAEEAAGLPPLSTLIEDVFATPTNALREQLAEVAAALPHKPH"
     gene            complement(4940..5332)
                     /locus_tag="PKL17_05505"
     CDS             complement(4940..5332)
                     /locus_tag="PKL17_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNK44216.1"
                     /translation="MSQQRTVISTGAAPAAIGPYSQAIAVSNLVFCSGQIPLSPESGQ
                     LVGAGDVAAQTEQVMKNLQAVLAAAGCSFADVARCTIYLSNMGDFATVNGVYGRYFPE
                     HPPARVTIAAAGLPRGALVEIDAIAVRP"
     gene            5412..6467
                     /locus_tag="PKL17_05510"
     CDS             5412..6467
                     /locus_tag="PKL17_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK44217.1"
                     /translation="MSIVSRQVVTPRLQIKCLDTGGSGQPVLFVHGNISTSEFFADLL
                     LKLPPAYRGIAPDLRGFGDTEAKPIDATRGVDDFVDDIASLIDSIEGLAGKKVVLCGW
                     SVGGAVVLRYAIKHPDRVAGLVLEAPMSPFGFGGTRDAVGTPCFADFAGSGAGTASPP
                     FVERLAQKDRSADSDFSPRKIVRGFFVKAGHQIAAAEEDRYVDEILKTQIGADHYPGD
                     AAGSANWPTVAPGTRGMNNAISPKYVNLSGFAKIPTHPKVLWIRGTDDQIVSDTSLFC
                     FGFLGKLGVIPGWPGDEAYPAQPMVSQMRTLLNDYRANGGSYEEVVLTDCGHSPHLEK
                     PTEFREKLLSFLHEIGV"
     gene            complement(6464..9220)
                     /gene="ppdK"
                     /locus_tag="PKL17_05515"
     CDS             complement(6464..9220)
                     /gene="ppdK"
                     /locus_tag="PKL17_05515"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNK44218.1"
                     /translation="MSAQTATPFQGPAKWVYLFSELDAAEQSCGSWDGVRGLLGGKGA
                     NLADMVRIGVPVPPGFVVSTAACNAFLAEGAKFPAGMWEEELLALRQVESLTGKRFGD
                     PQNPLLVSCRSGAKFSMPGMMDTVLNIGLNEEVAAGLAKLTGDSRFVYDSYRRLVQMF
                     GTVVLSLPDEPFEEVLAHARKAAGVENDASLHENDLRRITAEFKQIIARLAGHSMPDD
                     PYEQLRLATEAVFRSWNGKRAIDYRRASNIADNLGTAVCIVAMVFGNLGDTSGTGVAT
                     TRNVSTGENELEGDFLVNAQGEDVVSGTRQTERIAALGEKMPTIFAEFRDICGKLERH
                     YRDVQDVEFTIERGKLWILQTRDAKRTAQAAVRIAVELSDERLITPEEAVLRVSPSHI
                     EYFLHPQFDPKAKKERKAAGDLIATGLNVAPGAAVGEIVFDADTAERLAKVEKKAVIL
                     VRAETKPDDVHGILAARGVLTSRGGRTSHAALVTRQFGKPAVVGASALDIDAASRTFT
                     VLGRTFKEGDFVSIDGTSGEVFAGQLQTVSPDIRDPHLLRLLGLADRFRRLGVWANAD
                     YPRDAKKAREYGAQGIGLCRTEHMFFEPERLPHIQAMILARSDAERAEPLARLLPFQR
                     ADFDGLFRIMDGLPVTIRLIDPPLHEFLPSHDELLRDVAILETRVKLGDGEPELVQHE
                     LDVKRRMLHAVQFLREQNPMLGLRGVRLGIHLPELTKMQVRAIFEAACACKRDGIVVK
                     PKIMIPLVALSSELKMERTLLEEEAQKVMAEQGVRVDYQFGTMIEIPRAALTADQIAE
                     FAEFFSFGTNDLTQTTFGISRDDAEASFLVEYLSRGLLPKNPFATLDERGIGKLMDMA
                     VRLGRQSRPGLEVGICGEHGGDPESIALCHKLQLDYVSCSPYRVPVARLAAAHAALSR
                     KKPN"
     gene            9355..10485
                     /locus_tag="PKL17_05520"
     CDS             9355..10485
                     /locus_tag="PKL17_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNK44219.1"
                     /translation="MHDGQPEQAAQRMEVSELAHLIDVLWQAGYRVLGPTVGDGAIVH
                     GEVRTPNDLPVGIRDEQNGGHYRLVQRGDGAFFGHNVGPHSLKRELLPPRERLFSTIR
                     RGKQVTITNEPLSTEKIAFLGAKACELSAMLTQDRVFLGQNHPDAHYAARRRAMFIVG
                     VSCTQAGGTCFCTSMGTGPRVDAGADLALVEVVSDRGHYFLIESKTPAGHDVAKKLAL
                     APASVDDIRQAELRIADATTQMGRHLSTSGIKEQLAAAAEHPHWDDVASRCLSCANCT
                     LVCPTCFCNTVEDVTDLEGDHSERWRRWDSCFHEDFSYLHGGSVRHSVKSRYRQWMTH
                     KLSTWIDQFGQSGCVGCGRCITFCPVGIDITAEVLALKPTSP"
     gene            10482..10988
                     /locus_tag="PKL17_05525"
     CDS             10482..10988
                     /locus_tag="PKL17_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNK44220.1"
                     /translation="MKKTNLSLSPQTLSLPFFVGFSEDELAILADHCRRSHVPANTEV
                     VREGQVATEFFLVLDGKLVVETHVPGRGAVVLQTLHRGDVLGWSWLLANGSFAFSAKT
                     LTETELLIFDGTALLRTCDSHPRLGYLLMRRFAQVMSDRLRHTRIQLLDLYSPPPGGG
                     AFPALKKR"
     gene            10985..11854
                     /locus_tag="PKL17_05530"
     CDS             10985..11854
                     /locus_tag="PKL17_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNK44221.1"
                     /translation="MSDEPKPINMPKADECSALCPTPYRVLRYQRETDDTFTLTMEPE
                     SGDEALRFAPGQFNMLYAFPTGDVPISVSGAAADRKVLVHTIRAVGPVTRALSTLRRG
                     DSVLVRGPFGTPWPVEQAEDHDVVIIAGGIGLAPLRPVIYHLLAHRSRYRRVSLLYGA
                     RTPSDVLYPKQLAAWKKRADLQVEVTVDRGDPSWHGDVGVVTQLIDRVATDPSRTVAM
                     ICGPEVMMHYCRLALSQRGIAAKQIYLSMERNMKCGVGSCGHCQLGPFFLCKDGPVLD
                     YERLGPVFSVKEL"
     gene            11856..12647
                     /locus_tag="PKL17_05535"
     CDS             11856..12647
                     /locus_tag="PKL17_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNK44222.1"
                     /translation="MKAKARPRLAVWKFASCDGCQLSLLDCEDELLRLAQVVQFANFV
                     EASSKVSPGPYDLSLVEGSITTEHDRQRIADIRASSRILVTIGACATSGGIQALRNGA
                     DVAEFTRHVYAHPEYISTLATSTPMSAHVKVDYELFGCPISKSELLEVVAAFLQGRKP
                     RVYSHAVCVDCKLRGTPCLTVTRGEVCLGPVTHAGCQALCPSYGRGCFGCFGPKEASN
                     PSSLAAHLQRQSGQPDVALSRLLNSFAGQAPAFVLARKRLHGSSC"
     gene            12671..14044
                     /locus_tag="PKL17_05540"
     CDS             12671..14044
                     /locus_tag="PKL17_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNK44223.1"
                     /translation="MDHDSDSNSASESELRLRAGAKTRTIHVDYLARVEGEGALYVKT
                     RGDLVEDVQLRIFEPPRFFEAFLRGRLATEAPDLTARICGICPVAYQMSSTHAIEQAL
                     GIQITPDIRALRRLLYCGEWIESHGLHVYMLHAPDFLGKSDVVALAKDFPGLVRDGLE
                     LKKTGNEILRVLGGREIHPINIRVGGFYRLPTRAELLTLREPLLRARDHALATVRWVS
                     GFPFPDVSRNYDFVSLWHPDEYPLCEGQIVSSSGLRIAARDFEAHFVEQHVAHSNALH
                     AAKVSDGGAYLVGPLARYNLCGDKLPEIAKQAARDAKLPSRCQNPFQSIIVRAVELVF
                     ACDEALRIVEAYRPSGPSYVAAEPRDAEGAAATCAPRGLLYQRYKIRQDGTIAECKIV
                     PPTSQNQRTIEDDLRTVVSQEIRRPKAELTARCEQTIRNHDPCISCATHFLRLEIEDD
                     AKIQQNS"
     gene            14019..15392
                     /gene="glmM"
                     /locus_tag="PKL17_05545"
     CDS             14019..15392
                     /gene="glmM"
                     /locus_tag="PKL17_05545"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNK44224.1"
                     /translation="MQKSSKTPDRKLFGTDGIRGVANVPPMTIETAMRLGRAVARLYH
                     RPGHRGRIIIGKDTRLSGYMFEQAMAAGICSMGADVMLCGPLPTPGIAFLTSSMRADA
                     GVVISASHNPYYDNGIKIFAADGFKLPDSTEAKLEEMMGDSDETITRPGHDEIGRAQR
                     IDDARGRYIVFLKNSFPRKLSLDGLKIVVDCAHGAAYRVAPNVLSELGAQVIALGVGP
                     DGKNINDHVGALHTEAMCRAVVEQGAHVGIALDGDADRVIFADERGQIVDGDAIMALC
                     ARRLLARGELRKNTLVATVMSNLGLERALSDMGGKLLRTAVGDRYVVEAMRQNDLNLG
                     GEQSGHLVFLDHMTTGDGIIAALQVLTAMLEENQPLSSLASIMQPYPQVLLSVKVARR
                     LPLGELGTVQSLISSVESTLGQDGRVLVRYSGTEPKARVMIEGPDETLIQRYAQDIAS
                     ELVRACS"
     gene            complement(15408..16133)
                     /locus_tag="PKL17_05550"
     CDS             complement(15408..16133)
                     /locus_tag="PKL17_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNK44225.1"
                     /translation="MLMSVVMAGCAGHLEVRAPVEQPFDALIIPGCPTLADGALSRCL
                     QRRAVWAALLWERGQAQHFITSGSAVYTPFVEAEALAVAMTAMGVPADRIYLEPHALH
                     TEENVYNSLRIAKKKGWQRIGVASDRGQALGACQMLSDWHGQCGAFSMETSIVDHRRS
                     ELDGLLSSLRAPKVSEFLPLSQRERDRAKRQNRPARPPSFLLYPVMMMRRTLGKTPWL
                     PFHPEETPLLTWAEQKDVRRSPT"
     gene            complement(16224..19040)
                     /locus_tag="PKL17_05555"
     CDS             complement(16224..19040)
                     /locus_tag="PKL17_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK44226.1"
                     /translation="MSRCLSCHAPVPDGTEICGSCRTALVPRLDGEMLSGDETGPHYT
                     LSEEDLLQAESADTEVPFCGRKEELGRLLGCIEQSIKSHSLRSFLLIAGTGQGKTRLL
                     SELGRAATSYFAVSPDRVLQATVPGEGAAPLAVFIELIRRRCDLQKSEPIGAARDKLL
                     RLCRVLLPAVRATEVAHVLGELLGIPFPDGSAQPTNSQAASVRSESRMYSAIKRFFVA
                     DARRGPLLILLDDMDQASPETVNLVTYLLDGLSDLPVVVGLAARPEFLETFPDFGATS
                     SPPVRLQLNLLCDEEKLQLIGSIVGAEPEELPSWVVSHCQQFSESTPRAMVEFLRLLV
                     ETQVLSWQPGDGDRSLVPVWDEEKLSQVELPSSLPGVVEARLRAMAEGSRRLLEYAAV
                     AGENFHLGALLTLSRCEAYGLTELGDGDLNGPPLADIQDGDPERVAELSELCEQLIGQ
                     GVLMLVPNSQLGVEKELRFAYPPWQKTIYEQVESERRRRYHLLMAQFLLLQPDADRED
                     LQQRIARHYERAGEGLLAALSFERAAELSVKSGATGRAPRLLLRGLACLSSEHLSTRL
                     RLWQQVGRVLSLLGDLEAALSAWENVVRLGFALASRKERAQAFLEMARIVAHDPSRAL
                     RYIQRSLELFTHLQDQVGISDALDEEGQALVWLGKQSDGMDRLAQSLEMRRRLLDRKK
                     VAQSLLHVGLVEYQRGALDAAVSCYEEALRKSEDEPRLQAAAMTALGSVDLARGEVAA
                     ARARLEDALPLCERAGPSKELVTLLCCLGDAMLRDGLLSEAESRLLQAKELALRLLDQ
                     RGLAESKRLLGLIHLRRGDKQKALELCQRALERAQQSGLRSLIARGLLTLGEIHASTL
                     FDETVEGEHPAWDYLKRAVALLREGGDQIELAQGLAAFGKLLVERRKLGPGRAALREA
                     SQLAGRLRMKLHDELQPLISDLGG"
     gene            19146..20249
                     /locus_tag="PKL17_05560"
     CDS             19146..20249
                     /locus_tag="PKL17_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNK44227.1"
                     /translation="MSEERIPEPRRSRLSTYNQVALAAAATVALCYFGRLVIMPTLMG
                     VAFALLLGPFVDACARVRIPRSLSALLLITLTLTVMYAATHLLYDLVTEFLDELPRHS
                     AKFDAFFDAIRKKLDSLAKTTRLLGNTPEPSISVRQPIDWPKLVIGGANSLGEFLFIT
                     SFVPFITYFLLTWQNHLYRATVRMFSVDRRSEVERTMGQIASMMRSVLVGNLLVGLLI
                     GVLSTVLFALVHVPYFYILGFVSGLLSMVPYLGSVLAVLPPLLMGLGQLSGSGVLALG
                     MGVLLLHLLAINLLVPKMIGKRVQINPLAATLSLLLWGVLWGGIGLLLAVPIAAAIKI
                     VCDHIEPLHAIGAWLGDDLDEDPAPKASAAEHA"
     gene            20306..21745
                     /locus_tag="PKL17_05565"
     CDS             20306..21745
                     /locus_tag="PKL17_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNK44228.1"
                     /translation="MFSTALFALALISLPACQGSGRQKTVAAKKAAPTTAPSPPASPS
                     GLPALPPPATTSRTGGIELVGTTLPAALTPSQQSRRTPVVDAVNRAAPSVVSIFSEQK
                     PQYNPFGFFGLEDDDETGRTSLGSGVIVDPRGLIVTNEHVVAGAAKIRVLLADGRELP
                     ATLVGADQSFDLAVLKIEAVGKDNKEPKDLPSLKLGTASDLMIGETVIAIGNPFGLSH
                     TVTSGVISALHRMVRAKNRTYEDFIQIDAQINPGNSGGPLLNVNGELIGINTAVHSGG
                     PGIGFAIPIDRARTIVNDLLQFGRVRYGFVGVRVAPIHERRRQATGVLVTEVDAGSPA
                     EKAGLQPGDVITHIGDEATVSVERFWDRVHRILAGEDVRVRLTRGEVSVHTTALDPRE
                     MSQRAKKRLGLDVIDAQGRAVLVQRVSQGSIADRIGFQPGDAILQIGARQIRSQNDFV
                     TALGEIRPGTDTVMLVFRGQTSYYVTVPF"
     gene            21889..26460
                     /locus_tag="PKL17_05570"
     CDS             21889..26460
                     /locus_tag="PKL17_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNK44229.1"
                     /translation="MRSKRPTKALPAITASLLLSATIACDPPKPPKPAQVATPTTPQP
                     PPVEAPPLASDKVLAKSPLEAPPPPETAPPESGPAGPKPPLPPVPAELAPPASPRLSG
                     VSASDVVKTKVESFFAGKNSRRIHIQTDKPLYKPGETVWVRVWDVTTKALSGKHPGAG
                     MHVELINPRGAQIAKKKVREAGGSGQIDFALDGGIVGGEYKIKVKTFDGVQDERPLIV
                     STYEPPKLKLKLEFVRKAYGPGDEVTATIAVKRNTGEPLRNHGLTATVMLDGQALPQV
                     QLRTDEEGDGLVRFNLPNEISLGDGLLTVMADDGGITESIAKRVPIIVKKLNLALYPE
                     GGDLVYGLPGRVYFEAKNPIGKPADISGRILDENEQTVGSFESVRDGLGRVDFTPQQG
                     HRYTVTVDQPVGITDRFVMPTPQEQGCVIRSIDDLDGQQTATRVSVRCTDKRKVVVAA
                     TMRENLLDVAAVEADREPATVYLEPRGQGSEALGRTQGAVRVTVFDEAYQPLAERLVY
                     RQRRARLQVQVDPIKKSFVPREKVTLDITTRDVQGNPVPADVALSVVDDTVLSFADDK
                     TGHMLSRLYLEPELTGKVEEPNFYFDLSEKKSGMALDLLMGTRGYRRFEWRPVLGGLI
                     AQNTGKAKDETAQTVTSFSDLRKASRALPKPDKPQDVVLAQTKAPPPPPERKAHAAAE
                     REQLVVAVNKPMAGPAKTPVALGTVAATPPMRAGAGGGMVAPMAAPAMAAGAAPRPMA
                     GHADKADRMVATEDSGAPMKEEKAMAAAEPMMAKKKMAMGRAAAMDDLAGGDDMMGGP
                     VGFPMRHRDFQRPRPRPVEPWAPVRVFPQPTYQPDYVGPRTDFRETVFWAPSVQTGQD
                     GKAQLSFVLSDAVTSFRVQTEAIGGGAAGREETVIKSSLPFSMAVKLPVEVSEGDRIA
                     LPLILSNERDRALQVNLAASFGSLLKLERPVDRAQGSIAALSRDSLFYPLAVTGKKGK
                     TEVRFVADAGGLRDEFVREIAVAPRGFPQNISKSGRLKDNVVHEVELAGLMDGTSDAH
                     LKIYVSQVSTLIAGLDGMLSEPGGCFEQASSTNYPNVMILRYLKTAQIAAPRVMSRAS
                     GLLGDGYKLLTGYETPTKGYEWFGSTPGHEALTAYGLVEFTDMKQVFPEVSGAMIKRT
                     ADWLHNRRDGKGGYSRNPQALDSFGSASKAVTDAYITYSVSEAKQPGFEREIDAQEQV
                     ARDTQDGYVLALSTNTLLNAGRKDGGYAAAKRLVAMQDPSGAWKNASHSITRSGGRDL
                     QIETTALAILALLKSGQFDESVEKGMEWLMQHRSGHGAFGATQATVLALKAITAYSSA
                     RARAMTPGMVTLVVNGKEAGQLSYQPGDREPLTFAGLQKHLVAGKNRIELRHTGQTPL
                     PYSLGVSYRSDQPATSPDAKVSLVTALQRNQVKLGESVRLDVTLTNKTDQGLPMTLAR
                     VEIPGGLQFQTWQLKELREKGLIGFYETRPRQVNLYLRQMQPKQEVKLPLDLIATVPG
                     DYTAQASTAYLYYTNEHKTWVPGTRIAIE"
     gene            complement(26482..26877)
                     /locus_tag="PKL17_05575"
     CDS             complement(26482..26877)
                     /locus_tag="PKL17_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44230.1"
                     /translation="MHKNGVLWVLGSLGLSLVGVLALSCTDTTTDTPPAAVVDMRAPI
                     MDLAPSATITVQDDYYSPQEVTIPVGGKVRWVFRALSQHGVFPWDMGFPGSDTMSMGT
                     FENTFNQAGTFDYGCAIHGRAMPGRIIVK"
     gene            complement(26890..27615)
                     /locus_tag="PKL17_05580"
     CDS             complement(26890..27615)
                     /locus_tag="PKL17_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK44231.1"
                     /translation="MNAAAERNHAIYEDLWTHYELFPHSDWTYTWTEIAPLYAPDRTL
                     EIGPGMFPHVPIEGTHFVDLSRVALRALADRGGHCAVATTPLPYADARFDLVCLFEVL
                     EHVAEDEALLREICRVMTPGGHLFFSCPVNPDYWTYYDKVMGHERRYRGSELQERLYR
                     AGFVIEKVCPRHDRMDRWFGAMFGFGMRYLPGFTANIVRRYLPKVAALPWQWYEGSDF
                     AQAETMGGVTVRARKRLPSELPI"
     gene            27733..30045
                     /locus_tag="PKL17_05585"
     CDS             27733..30045
                     /locus_tag="PKL17_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNK44232.1"
                     /translation="MSRQGFILQASMRLMAGQPVLLLYGRLAEGETFLVRDDRQRACF
                     FVRTCDGEAVQKLAGPTVKLLKTPRQSLAKEPVSRVELQTPVSAAALSHRLHAAGIAT
                     FEADLNMVMRYLIDRGIRGSVEIHGSARRGRPSEEGVDLVFDNPELMPGSVLPPLKPL
                     TIRLQEGSDGTLAAAAIADELSTVVLHRKPTTSSDATAFPDERALLGALIQRIASKDP
                     DLLVGWNLLTEDLPLLRDAARRCRLSLPLARGPGDLQYQPGVGRGQTPRAVLSGRVVF
                     DVAPIWRHVKGAGYEDSLAALLDGETGKHAPSEVDTANPDELGRLRREAEGLRDLLHR
                     YGLLDLAIQRSRLTGLPMDRATASVAAFDFLYLVELGRRGYVAPTFERPTELGEHVIG
                     GHIIAPLPGLHKNVLVFDFRSLYPSVIRTFQIDPLGLLSDGDGGEPDPIVAPSGSKFR
                     RQRGILSVILDQLLPLRSAAKSQGNEAQAQAIKLLMNSLYGVLGSPACRFHKPELANA
                     VTSFGRELLQFSQARLESYGHKVLYGDTDSLFATTNLDDSATEAQLRSLGETLTARLN
                     ADLSDYIEKTWRVESRLELALHSVYRRLLLHRQRGKDKGAAKRYAGLLLKGDQCRLHI
                     VGLEAVRRDSSELCRTVQQTLYSMLFASASKEEIAAYLHRIIAELLAGKLDAMLVYRK
                     ALRREPADYVAKGAPHVVVATRHGLAPGSLVPYVMTTAGAEHPAHRQSPIDYQHYLEK
                     QVRSVAEPVLAHYGLPFGQKRQSDPQLRLL"
     gene            complement(30047..30802)
                     /locus_tag="PKL17_05590"
     CDS             complement(30047..30802)
                     /locus_tag="PKL17_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK44233.1"
                     /translation="MQKKLPQGFGSTTDDAQPLAGQVVLVTGGARRLGRAICETLWQQ
                     GASIVIHYHRSMADAQALASQLGSRARIVQADLRDRAATENMFASVLAECLRIDAVVC
                     NAAAFVRTPIETLDDAAWDDLIAVNLTAPRRCLQLGIAAGAKVIVNVADVGATQAWRG
                     FAAYGVSKAGLLQLTKIAAKELIGRVRVNAVLPGFVLPPDDASEDELARLSAKWAGYP
                     AGSPLDVARAVAFLLREPFLTGVCLPVDGGQSL"
     gene            30901..32067
                     /gene="ftsY"
                     /locus_tag="PKL17_05595"
     CDS             30901..32067
                     /gene="ftsY"
                     /locus_tag="PKL17_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNK44234.1"
                     /translation="MFSVTLSSAQATSAAPSVSSTTVLVALLVVAIAVIAVVLLRRKP
                     QLPDQPDTKSAGEPNLLPSRATTAAPQPSDTAATPSPKSKAGHDELKAGLSKTRAGFI
                     ARIAELLSKKQISDDLLPQLEEVLLTADIGPKTAEHLFSQVRSTLSRSELKDPAAVWG
                     VIKDESRRLLNVDAPAIDIAAHQPFVLLVIGVNGVGKTTTIGKLAAQHMAQGRKVILA
                     AGDTFRAAAVEQLEIWGQRAGVPVVRGKEGGDPSSVIFEAIKRAQREQIDLVIADTAG
                     RLHVKEQLMAELQKVRRVIKKADPTAPHETWLVLDATQGQNTIAQARTFKEAMEITGI
                     VLTKLDGTAKGGVILGITHELGVPVRYIGVGEKIGDLRAFDAEDFVDVLYERSA"
BASE COUNT         6386 a        10291 c         9812 g         5890 t
ORIGIN      
        1 ccgctggttt ttgtttgcag aaattggcgg ctccccggtc ttccgctggt ttttgtttgc
       61 agaaattggc ggctccccgg tcttccgctg gtttttgcgc gcggaaatgg gcggcacctg
      121 ggtctgccgc tggtttttgt ttgcagaaat cggcggcacc tgggtctgcc gccgtttttg
      181 ttgcggttgt gcgggccaag gtcgcgggtc ggtcagcggc ggcagcgggc ggctgtcgag
      241 acggtccgac cagggcgccc ggcttgacca aacggcggtg acagggctaa aaaggccgca
      301 gtcctttcat ggggaatcca cagtccatga cacagcccgc actgcctccc ggcgcgattg
      361 tcgcggaaaa ggcgccgccg ccgttctcgc tgctcgacta cgaaggaaac cttcgggccg
      421 atgcagatcc cagcttggtt cctgacattt ccgacgccga ctgcctgcgt ctgtatcggg
      481 aatttatcaa gcttcgtctg gtcgatgacc gctacctcaa gcttcagcgg caggggcggc
      541 tcggctttta catgcagtcc accggcgagg aagcgaccca gcttggcgtc gcctatgccc
      601 tgcgccaaac cgactggatt tacccgtcgt atcgcgagcc cggcatcgcg tttttccgtg
      661 gctactcgat tcacgacttt acaaaccagc tgtttggcaa cgccgaagac ccgatcaagg
      721 gtcggcagat gccggttcac cacagcttcc gcgccggtaa ctttgtgtcg atttcgtcgc
      781 cggtcggaac gcagattccg caggccgtcg gcaccgcccg cgcggcacag atcctgaagc
      841 acgacaccgt gacgtcggtg ttttttggcg aaggcacgac ctcgccaggc gacttccacg
      901 tcggtctcaa ctttgcgggc gtctggaaag cgccggtcat cttcgtctgc cgcaacaacg
      961 gctgggcgat ttcgaccccg atttccgtgc agaccgcgtc cccgacgatt gcccacaagg
     1021 cgctcggcta cggtttcccc ggcttcctgg tcgacggaaa cgacatcctg gcgatgattt
     1081 cggcgacgcg gattgctgcg gcgcgggcgc gggccgggga aggtccgacg ctcatcgaag
     1141 cgctgaccta tcgtcgggcg gcgcactcgt cgtcagatga cccgtcggtc taccgcaaag
     1201 gcaaagacga cgagatcaaa gagtgggaac ggcgcgatcc ggtcgcccgc tttgcccgct
     1261 acctggaacg gcgcggcatc ctgacgccgg cgattgacgc ccagtaccgc gacgagatca
     1321 cgcaagagat aagcgccgcg ctggcccaca tcgagcacgt cggaacgccg ccgctcatgt
     1381 cgctgattga agatgtgtac gcccagccga cggccccact gcgcgaacag ctcgccgagc
     1441 tgcttacgct gccccgtcag aagctaggac acggttagac cgccgattgg caaggagttt
     1501 tcttcgacat gcccgtatac aacatcatcc aagcggtcaa cgacgccctg cgcatcgaga
     1561 tgcgcaaaaa ttcaaacatc gtcgtcctcg gtgaagacgt cggcaacttc ggcggcgtgt
     1621 tccgcgcgac gagcggcctg caagaagagt tcggcaaaga ccgggtcatg gacacccctc
     1681 ttgccgaggc tggcatcatc ggtgcggcga tcggtatgtc gctttacggt ctgcgccccg
     1741 tcgccgaaat ccagtttgcc gacttcatct tcccggcgtt tgaccagatc gtgaacgagc
     1801 tggccaagtt ccgctatcgc tcgggcggcg agtatccagc gccgatggtc atccgcacgc
     1861 cgtatggcgg tggcatccgc ggcggtcact atcactcgca gtcgcccgag gcgtactttg
     1921 cccatacgcc gggtctcaag atcgtgattc cgtcgaatcc gtatgacgcc aagggtctgc
     1981 ttctgcaagc gatgcgcgaa gaagacccga tcctgtttat ggaacccaag cgcgtctacc
     2041 gggcgtcacg cggcgaagtg cccgagggtg agtacacgat cccgctcggc caggccaaag
     2101 tcgttcgccc cggcaaaggc gtgaccgtgc tgtgctacgg atcgatggtc cacaccgtca
     2161 tggaagcggt cgaaaagcag aacgccgccg agtttgaccc cgaagtcatc gacctgcgga
     2221 cgatcatccc gttcgacgcc gagacgatcc tcgattcggt caaaaagacc gggcgcgtcg
     2281 tcatcgtcta cgaagccccg aagacctgcg gctttggcgc cgagcttgcc gcgtttatcg
     2341 ccgaaaaagc gatcctgcac ctcgaggcgc cgatcgcccg ctgtgctggc ctggacaccc
     2401 cgttccccta cacgctcgag ctggaatacc tgccatcgcc cgagcgcgtc gccaaagcaa
     2461 tcgccgacac cgcgaggtat taagccatgg ctctcgagtt caaactcccc gacattggcg
     2521 aaggcgtcgt cgaaggcgag atcgttcgct ggctggtcaa agaaggcgac gtgctgcgcg
     2581 aagaccagcc gatggtcgaa gtcatgaccg acaaggcgac cgtcgaaatc ccgacgccac
     2641 gcgccggtcg ggttcacaag ctgatggtcg atgccggcaa gctgtgcgcc gtgggtcagg
     2701 tcatgatcgt gatcgacacc ggcggcaccg ccagcccgtc ggcagcaccg acgcccgcgg
     2761 cccaagcccc cgtgacgcaa gccgcccccg cacaagccgc tcaggcgcag gtttcagcgc
     2821 ccgttcaaac ggtgtcagcc gccgaagccg ccggtcgcaa ggttttggcc accccggcga
     2881 cgcgacagct tgcacggaac ctgggcatcg acattcgcac ggtgctcggc agtggccctg
     2941 gtggtcgcat cacccgtgac gatgtccgtc ggttgagcga aaaagccacc gccccggcac
     3001 aagcggcacc gatcccggtg caagcccccg cagcggcgca aaagccagcc gccccagcgc
     3061 cgcgtccggc ggcagagcct gtcgcggtgg ctgtcagcgc cgatgacgag cgaattgcct
     3121 tccgcggcgt gcgcaaaaag atcgccgaaa acatgctgcg atcgaagcag tcggcggcgc
     3181 actttaccta cgtcgaggaa gtggatgtca ccgacctcgt cgcgctgcgc aaacgggcgc
     3241 agaagcgggc gcaagagcgc ggcgccaagc tgtcgttttt gccgttcatc atcaaagcgg
     3301 tcgtctcggg gctgaagaag tacccgatct tgaacgcgac gctcgaccag cagcgggaag
     3361 agatcgtgct gcgcaagcag tacaacattg gtgtggcagc agcgacggat tcggggctga
     3421 tcgttccggt cttgcgcaac gccgacaaga tgtcgctgtt ccagatcgcg tcggagctgg
     3481 agccgctcgc cgagcgggca cgcaccggaa aagccagccg tgacgagctg accggatcga
     3541 cgtttaccat ctcgtcgctc ggcaagctgg gcggtgtgct ggcgacgccg atcatcaact
     3601 tccccgaagt ggcgatcctg ggcgttcaca aaatcaagga aaccccagca gtgcgcgacg
     3661 ggcagatcgt ggtgcggcag gtgatgaacc tgtctatttc gctcgatcac cggatcgtcg
     3721 atggctacga aggcgcgatg ttccttcagc atgtggtttc gctgctcgaa gacccgacgc
     3781 tgatgtttat ggagctgacc taaccgatgc gcaacgcccc gccttctttt ggctttgcgc
     3841 cgcccgccga gcagtttgtg cccaggcaat acctggcgcc aaatggcagc tttgacccga
     3901 cgcaggttgc agcactcggc atcccgcggg atcagcttct gaagctgtat cgcggcatgc
     3961 tgctgattcg gatcatcgac gaacggatgc tcctgctcca gcgccaaggt cggatcagct
     4021 tctatggcga agcgcgcggc caggaagcgg cggtggtggg aacagcggcg ggccttggac
     4081 cgcaagacta catcctgccg gcgctgcgcg aagcgggagc ggcgctgtat cgggggctgc
     4141 cgctccggac gtatatctcg cagatttacg gcaacgaaaa cgacgtcacc aaaggccggc
     4201 agatgccgtg tcaccccggt acgcggcagg cccgttacgt cacgatgtcg tcgtgcatcg
     4261 cgacgcagct gccgcatgcg gtcgggatgg cgatggcggc caaaattcgt cgcgacgacg
     4321 tggtcattgc cggttacatg ggcgatggcg cgacgagcga aggcgatttc cacgtcgcac
     4381 tcaactttgc cgcggtctac aaagcgcctg tggtgttcgt ctgtcaaaac aaccagtggg
     4441 ctatatcgac gccggtggcg ggacagacgg catcaccaac gatggcgcac aaagcgctgg
     4501 cctatggcat tccggcgctg cgcgtcgatg gcaacgatgt gctcgcgtgt tatacggcga
     4561 tgcgcgacgc cgtggcgtat gcccgcagcg ggggcggccc gatgttcgtc gaagcgctga
     4621 cctaccgggt gagcgcacac tcgacgtcgg atgatccgtc gcgttatcgc gatgaatcgg
     4681 tgacggcggt gtggaaaacc gagcgcgatc cgatccagcg ctatcggcac tttctgcggg
     4741 tgattggcgc gctgaccgac ggtgttgacg aagcgctgca cgaagcgatc gaagccgaga
     4801 tcaaagccgc ggtgctggcg gaagaagcgg cagggctgcc accgctatcg acgctgatcg
     4861 aagacgtgtt tgcgacaccg acgaacgccc tgcgtgagca gcttgccgaa gtcgccgccg
     4921 ccctgcccca caagccgcac tagggacgaa cagcgatggc gtcgatttcg acgagcgcac
     4981 cgcgcggcag accggcggca gcaatcgtga cgcgcgccgg cggatgttcc ggaaagtagc
     5041 gaccgtagac gccgttgacg gtggcaaagt cgcccatgtt ggacaggtaa atggtgcagc
     5101 gggcgacgtc ggcaaagctg caacccgctg ccgcgagcac cgcttgcaga tttttcatca
     5161 cctgctcggt ctgggcggcg acatcaccgg cgccgacaag ctgtccactt tccggcgaca
     5221 gcggaatctg ccccgaacaa aacaccaagt tggacacagc gatggcctgc gaatacggcc
     5281 caatcgccgc cggggcggcc cctgtcgaga tcacggtacg ttgttggctc atgccgcgag
     5341 cctatcttac atccgcccac ggcgccaaac gtctggtcgc gcgatacagc gacagcgtat
     5401 gatttttggc catgagcatc gtatcccgac aagtcgtcac tccacgtctc cagatcaagt
     5461 gccttgatac cggcggcagc ggccagcctg tgctgtttgt ccacggcaac atctcgacca
     5521 gcgagttttt tgccgacctg cttctgaagc tgcccccggc ctaccgcggc atcgcgccgg
     5581 atctgcgcgg ctttggcgac accgaagcca aaccgatcga cgcgacgcgc ggcgtggatg
     5641 atttcgtcga cgacatcgcc agcttgatcg attcgatcga agggctggcg ggcaagaaag
     5701 tcgtgctgtg tggctggtcc gtcggtggcg cggtggtgct gcgctatgca atcaaacacc
     5761 ccgacagagt cgccggactg gtgcttgaag caccgatgtc gccgtttggc tttggcggca
     5821 cccgtgatgc cgtcggaacg ccgtgttttg ccgactttgc cggatcgggc gccggaaccg
     5881 ccagcccccc ctttgtcgag cggcttgcgc aaaaagaccg cagcgccgac agcgattttt
     5941 cgccgcgcaa aatcgtccgc ggtttcttcg tcaaagcggg gcatcagatc gcggctgccg
     6001 aggaagatcg ctacgtcgac gaaatcttga agacccagat cggcgccgat cactatcctg
     6061 gcgatgcggc tggatcggcg aactggccga cggtggcacc gggaacgcgc ggcatgaaca
     6121 acgcgatttc gcccaagtac gtaaacctgt cgggctttgc caagattccg acgcatccaa
     6181 aagtgctgtg gattcgcggc accgacgatc aaatcgtgtc ggatacgtcg ctgttctgct
     6241 ttggtttcct gggcaagctg ggggtgattc cgggctggcc gggcgacgaa gcgtatccgg
     6301 cacagccgat ggtgtcgcag atgcggacgc tgctaaacga ctaccgcgca aacggtgggt
     6361 cgtatgaaga agtggtgctg acggactgcg gacactcgcc gcatctggaa aaaccgacgg
     6421 agtttcgcga aaagctgctg tcgttcctgc acgaaatcgg cgtctagttc ggttttttgc
     6481 gactgagggc ggcgtgcgcg gcagcgagtc gagcgacggg cacgcggtac ggtgaacacg
     6541 acacgtagtc gagctggagc ttgtggcaca gcgcgatgct ctcggggtcg ccaccgtgtt
     6601 cgccgcagat gccgacttca agtcctggcc ggctctgccg tccgagccgc accgccatgt
     6661 ccatcagctt gccgatgccg cgctcgtcga gcgtcgcgaa cggatttttc ggcaaaagac
     6721 cccgcgacag atattcgacc aggaagctcg cttcggcatc gtcacgcgaa atcccgaacg
     6781 tcgtctgcgt cagatcgttt gtgccaaacg aaaagaactc ggcaaattcg gcgatctggt
     6841 cggcggtgag cgccgcgcgc ggaatttcga tcatggtgcc gaactggtag tcgacgcgga
     6901 ccccttgctc ggccatgact ttttgcgctt cttcttcgag cagggtgcgc tccatcttca
     6961 gctcactcga cagcgcgacg agcggaatca tgattttcgg ctttacgacg atgccatcgc
     7021 gcttgcaggc gcacgccgcc tcgaaaatcg cacggacctg catcttggtc agctcgggaa
     7081 ggtggatgcc gagtcgaacg ccgcgcagac cgagcatcgg gttttgctcg cgcagaaact
     7141 gcacggcatg cagcatccgt cgcttcacat ccagttcgtg ttggactaat tctggttcgc
     7201 cgtcgcccaa cttgacgcga gtttccaaga tcgcgacatc gcgcagcagc tcatcgtggc
     7261 ttggcaaaaa ctcgtggagc ggcggatcga tcaggcgaat cgtcaccggc aggccatcca
     7321 taatccggaa cagcccgtcg aagtcggccc gttggaacgg caaaagacgc gccagcggtt
     7381 cggcccgttc ggcatcgctg cgcgccagga tcatcgcttg gatgtggggc agccgctcgg
     7441 gttcaaagaa catatgctcg gtacgacaca gtccaatgcc ttgggcgccg tattcgcggg
     7501 cttttttggc atcgcgcgga tagtcggcgt tggcccacac cccgaggcga cggaaccgat
     7561 cggccagccc gaggaggcgc aaaagatgcg gatctcgaat gtcgggtgag acggtctgaa
     7621 gctgaccggc gaagacttca ccgcttgtgc cgtcgatgga aacgaagtcg ccttctttga
     7681 acgtgcggcc taggacggta aaggttcgac tcgcggcatc aatatcgagc gccgaagcac
     7741 cgacgacggc aggcttgcca aactgccggg tgacgagcgc cgcgtggctg gtgcgaccgc
     7801 cacggctggt gagcacgccg cgcgccgcca agatgccgtg cacgtcgtcg ggtttggttt
     7861 cggcgcgaac caggatgacg gctttctttt cgactttggc cagtcgttcc gcggtatcgg
     7921 catcgaagac gatctctccg acggcagcac ccggtgcgac attgagtccg gtggcaatca
     7981 gatcgccggc tgctttgcgc tcttttttgg ctttgggatc aaactgcggg tgcaaaaagt
     8041 attcgatgtg gctcggcgag acacgcagga cggcttcttc gggcgtgatc agtctttcgt
     8101 cggaaagctc gactgcgatg cgaacggcgg cctgggcggt gcgcttggcg tcgcgggtct
     8161 gcaaaatcca cagcttgccg cgctcgatgg tgaactcgac gtcctgaacg tcgcggtagt
     8221 ggcgctctaa cttgccgcag atgtcgcgga actcggcaaa gatcgtcggc atcttttcgc
     8281 ccagcgcggc gatgcgttcg gtctgacggg tgcccgatac gacgtcttcg ccctgcgcgt
     8341 tgaccagaaa atcgccttcg agttcgtttt cgcccgtcga gacgttgcgc gtggtggcga
     8401 cgccagtgcc cgaagtgtcc ccgaggttgc caaacaccat cgcgacgatg cagacggcgg
     8461 tgccgaggtt gtcagcaatg ttcgaggcgc ggcggtagtc aatcgcgcgc ttgccgttcc
     8521 acgaacggaa gaccgcttcg gtggcgagcc ggagctgttc atacggatcg tcgggcatgc
     8581 tgtggccggc gaggcgggcg atgatctgct tgaactcggc ggtgatccgg cgcaggtcgt
     8641 tttcatgcag cgaagcatcg ttttcgacgc cagccgcttt gcgggcgtgg gcgagcactt
     8701 cttcaaacgg ctcgtcggga aggctcagca cgacagtgcc gaacatctgc acgagccgtc
     8761 gatacgagtc ataaacaaag cgcgaatcgc cggtcagctt ggcaagtccc gccgcgacct
     8821 cttcgtttag gccgatgttt aggactgtgt ccatcatgcc cggcatcgaa aacttggccc
     8881 ccgatcggca ggacacgagc agcggattct gcgggtcgcc aaagcgcttg ccggtcagcg
     8941 actcgacttg gcgcagcgcc aaaagttctt cttcccacat cccggcggga aacttggcac
     9001 cttcggccaa aaaggcgttg caagccgccg tcgaaacaac aaatcccggt gggacgggca
     9061 cgccgatgcg aaccatgtcg gcaaggtttg cgcccttgcc accgagcagc cctcgcaccc
     9121 catcccagga accgcacgat tgctcggcgg cgtcgagttc ggaaaacagg tacacccact
     9181 tggccgggcc ttgaaagggg gttgcagtct gagcggacat gatgagatac ctccgagcgt
     9241 cgttaaggaa tctgcgtaac tcttcgatct gcgaataatt tgggtacgcg cgcagggtaa
     9301 caccagagcg ggcgcgcaaa tcatcgcagc agcgagccaa ggacagaggg gacgatgcac
     9361 gacgggcagc ccgagcaagc cgcacaacgg atggaagtca gcgagcttgc gcacctcatc
     9421 gatgtgctgt ggcaagccgg ctaccgcgtg ctggggccca cggtgggtga tggggcgata
     9481 gtgcatggcg aggtgcgcac gccaaacgac ctgcccgtcg gcattcgcga tgaacaaaac
     9541 ggcggtcact atcgactggt tcagcggggt gacggcgcgt tctttggcca taacgtcggg
     9601 ccgcactcgc tcaagcgcga gcttttgccc ccgcgagagc ggctgttttc gacgattcgc
     9661 cgcggcaagc aagtgacgat cacaaacgag ccgctgtcga cggaaaagat cgcgtttctg
     9721 ggagccaaag cctgcgaact ttccgcgatg ctgacgcaag atcgggtgtt tttgggccaa
     9781 aaccatcccg atgcgcacta cgcggcacgc cggcgcgcga tgttcatcgt cggagtaagc
     9841 tgcacgcaag cgggcggcac atgtttttgc acgtcgatgg gaacggggcc gcgcgtcgat
     9901 gcgggcgccg atctcgcact tgtcgaagtg gtgtcggacc gcgggcacta ttttctgatc
     9961 gaatccaaga cgcctgccgg acacgatgtg gccaaaaagc ttgccctggc gccagccagc
    10021 gtcgacgaca ttcgccaagc cgagctgcgc attgccgatg cgacgaccca gatgggacgg
    10081 catctgtcga cgagcggaat caaagaacag cttgctgcgg cagccgaaca tccgcactgg
    10141 gacgacgtcg cttcgcgctg tctgtcgtgt gccaactgca cgctggtctg cccaacctgt
    10201 ttttgcaaca ccgtcgaaga tgtcaccgac ctagagggcg atcactccga gcgctggcga
    10261 cgctgggatt cgtgtttcca cgaagatttt tcctacctgc atggcggatc ggtccgtcac
    10321 agcgtcaagt cgcgctatcg gcagtggatg acccacaagc tgtcgacgtg gatcgatcag
    10381 tttggccagt cgggctgcgt cggctgcggt cgctgcatca cgttttgtcc cgtcgggatt
    10441 gatatcaccg ccgaagtctt ggccctaaag cctacgtcac catgaaaaag acgaatcttt
    10501 cgctgtcacc acagacgctg tcgctgccgt tcttcgtcgg attttccgaa gacgagctgg
    10561 cgattttggc cgaccactgt cgacgaagcc acgttccggc caacaccgaa gtcgtgcgcg
    10621 agggccaggt agcgaccgaa tttttcttgg tgctcgatgg aaagctggtg gtcgaaaccc
    10681 atgtccccgg tcgcggcgcg gtggtgcttc agacgctgca ccgcggtgat gtgctcggtt
    10741 ggtcgtggct tttggcaaac ggaagctttg ccttttcggc gaagacgctg accgagacgg
    10801 agcttttgat ttttgacggc acggcgctct tgcgcacctg tgacagccat ccgcggctcg
    10861 gttatctgct gatgcggcgc ttcgcccaag tgatgagcga tcggctgcgt cacacccgaa
    10921 ttcagctttt ggacctgtat tcgccgccgc cgggtggcgg tgcttttccg gcgctaaaaa
    10981 aacgatgagc gacgagccga aaccgatcaa catgccaaaa gcggacgagt gctcggcgct
    11041 ttgcccgacg ccttatcggg tcttgcgcta tcagcgtgag acggacgata cctttacgct
    11101 gacgatggaa ccggaatcgg gcgacgaggc acttcgcttt gcgcccggtc agttcaacat
    11161 gctgtatgca tttccgacgg gcgatgtgcc gatttcggtc agcggtgccg cggcagatcg
    11221 aaaagtgctc gtgcacacga ttcgcgctgt cggtccggtg acgcgggcgc tatcgacgct
    11281 cagacgaggc gatagcgtgc ttgtgcgcgg accgtttgga acgccgtggc ccgtcgaaca
    11341 agccgaagat cacgatgtgg tgatcatcgc ggggggcatc ggtctggcgc cgctgcgacc
    11401 ggtgatctat cacctgctcg cgcatcgctc gcggtatcgt cgggtgagcc tgctttacgg
    11461 tgcgcgcacg ccgagcgacg ttctctatcc caagcagctt gccgcgtgga aaaagcgcgc
    11521 cgatctgcaa gtcgaagtca ccgtcgatcg cggcgatcca agctggcatg gcgacgtcgg
    11581 tgtcgtgacc cagcttatcg atcgggtagc caccgatccc agtcgcaccg tcgccatgat
    11641 ctgtggtccc gaagtgatga tgcactactg tcggcttgcg ctgagccagc gcggcatcgc
    11701 ggcaaagcaa atctatctgt cgatggaacg caacatgaag tgcggcgtcg ggagctgcgg
    11761 tcactgtcag cttgggccgt ttttcctgtg caaagacggg ccggtgctcg actacgagcg
    11821 gcttgggccg gtgttctccg tcaaggagct gtagcgtgaa agccaaagcg cgtccccgac
    11881 tggcggtgtg gaagtttgcg tcctgcgatg gctgtcagct cagcttgctc gactgtgaag
    11941 acgaactttt gcgcctggcc caagtggtcc agtttgcaaa cttcgtcgaa gccagcagca
    12001 aggtttcccc cggaccctac gacctctcgc tcgtcgaagg atcgatcacc accgaacacg
    12061 atcggcagcg catcgccgac atccgcgcaa gctcgcgcat tttggtcacc atcggggcct
    12121 gcgcgacatc gggcggaatc caagcgctgc gcaacggcgc cgacgtcgcc gagtttaccc
    12181 gccacgtcta cgcgcatccc gaatacatct cgacgctcgc gacgagcaca ccgatgtcag
    12241 cccacgtcaa agtcgactat gagcttttcg gctgcccgat cagcaaaagc gagctgttgg
    12301 aagtcgtcgc cgcgtttttg caaggtcgca agccacgggt ctattcacac gcggtctgcg
    12361 tcgactgcaa gctgcgcggg acgccgtgcc tgacggtgac gcgcggcgaa gtctgtcttg
    12421 gcccagtcac ccacgcaggc tgccaagcgc tgtgccccag ctacggtcgc ggctgttttg
    12481 gctgctttgg ccccaaagaa gcgagcaacc cgtcgtcttt ggccgcccac cttcagcgcc
    12541 aaagcggcca gcccgatgtc gcgctctcgc gacttttgaa ctcgtttgcc ggccaagcgc
    12601 ccgcctttgt tttggcaaga aagcgcctcc acggctcaag ctgctaagct gcgcccgact
    12661 ccacttcacc atggaccacg acagcgattc caactcggca agcgaaagcg agctgcgcct
    12721 ccgcgccggg gccaagacgc gcaccatcca cgtcgactac cttgcacggg tcgagggcga
    12781 aggcgcgctg tacgtcaaga cccgcggcga tctggtcgag gacgtacagc tgcggatttt
    12841 tgaaccaccg cgcttttttg aagcgttttt gcgtggccga ttggcgacgg aagcgcccga
    12901 cctgacggcc cgcatctgcg gcatctgccc ggtggcgtat cagatgtcgt cgacgcatgc
    12961 gatagagcaa gcgctcggca tccagatcac cccggacatt cgcgcgctgc gtcggctctt
    13021 gtactgcggc gagtggatcg aaagccacgg cctgcatgtt tacatgctgc acgcgccgga
    13081 cttcctcggc aaaagcgacg tggtggcgct ggccaaagac tttcccggcc tggtgcgcga
    13141 cgggctcgaa ctgaaaaaaa ccggcaacga aatcctgcgc gtgctcggtg gccgtgaaat
    13201 ccatccgatc aacattcgcg tcggtggttt ttatcggctg cccacccgcg ccgaacttct
    13261 gaccctgcgt gaaccacttt tgcgggcccg cgatcacgcg cttgcaacgg tgcgctgggt
    13321 gtcggggttt cccttcccgg acgtgtcgcg gaactacgac tttgtcagtc tgtggcatcc
    13381 cgacgaatat ccgctgtgcg aaggccagat cgtttcgtcg tcgggtctgc gaatcgctgc
    13441 gcgtgacttt gaagcccact ttgtcgaaca gcatgtcgcc cactcgaatg cgctgcatgc
    13501 cgccaaagtg tccgacggtg gcgcgtattt ggtgggtccg cttgcgcgtt acaacctgtg
    13561 cggcgacaag ctgcccgaaa tcgccaagca agcagcccgc gacgccaagc tgccttcgcg
    13621 atgccaaaat ccgttccaaa gcatcatcgt gcgcgccgtc gagctggtct ttgcctgcga
    13681 tgaagcgctg cgaatcgtcg aagcctatcg cccgtcggga ccatcctacg tcgccgctga
    13741 gcctcgcgat gccgagggag ccgccgccac ctgtgcgccg cgtgggctgc tctaccagcg
    13801 ttacaaaatc cgccaggacg gcaccatcgc cgagtgcaag attgtgccgc ccacttcaca
    13861 aaaccaacgc accatcgagg acgatctgcg cacggttgta agccaagaaa tccgtcgacc
    13921 caaggcggag ctgacggcgc gctgcgagca gacgatccgc aaccacgacc cctgcatctc
    13981 gtgcgcgacc cattttctgc gcctggagat cgaggacgat gcaaaaatcc agcaaaactc
    14041 ctgaccgcaa gctgttcggc accgacggca ttcgcggcgt cgccaacgtc ccgccgatga
    14101 ccatcgaaac cgccatgcgg ctcggtcgcg ccgtcgcccg gctttaccac cgcccaggcc
    14161 accgaggccg catcatcatc ggcaaagaca cccgcttgtc gggctacatg ttcgagcagg
    14221 ccatggccgc cggcatttgc tcgatgggcg ccgatgtcat gctgtgcgga ccgctgccga
    14281 cgccgggcat tgcgtttttg acctcgtcga tgcgcgccga tgctggtgtg gtgatctcgg
    14341 cgagccacaa cccctactac gacaacggca tcaaaatttt cgcggcggac ggcttcaagc
    14401 tgccggactc gacagaagcc aagctcgaag aaatgatggg ggacagcgac gaaacgatca
    14461 cgcggcccgg tcacgacgaa atcggtcgcg cccagcgcat cgacgacgcc cgcggtcgct
    14521 acatcgtgtt tctcaaaaac tcgttcccgc gcaagctgtc gctcgatggg ctgaaaattg
    14581 tcgtcgattg tgcccacgga gccgcctatc gcgtcgcccc caatgtgctc tcggagcttg
    14641 gggcccaggt gattgcgctg ggcgtcggcc cggacggaaa aaacatcaac gatcacgtcg
    14701 gtgcgctgca tacagaagcc atgtgccggg ccgtcgtcga gcagggcgcg cacgtcggta
    14761 tcgcgctcga cggtgacgcc gatcgggtta tttttgccga tgagcgtggc cagattgtcg
    14821 acggtgatgc gatcatggcg ctgtgcgcaa ggcggctttt ggcacgcggc gagctgcgca
    14881 aaaacacgct ggtggcgacg gtgatgtcaa acctggggct ggaacgcgcg ctgtccgaca
    14941 tgggcggcaa gctgctgcgg actgccgtcg gcgatcgcta cgttgtcgaa gcgatgcggc
    15001 aaaacgatct caacctcggt ggtgaacaga gcggacacct ggtgttcctc gatcacatga
    15061 cgacgggcga cggcatcatc gcggccttgc aagtgctgac agcgatgctc gaagaaaatc
    15121 agccactttc gtcgctggcc agcatcatgc agccgtatcc acaggtgctt ttgtcggtca
    15181 aagtcgcgcg ccgcttgccg ctcggtgagc ttggcacggt gcagtcgctc atcagcagcg
    15241 tcgaaagcac gctcggccaa gacggacggg tgctggtgcg ctactcggga accgaaccca
    15301 aagcacgggt gatgatcgaa gggcccgacg agacgctgat tcagcgctat gcccaggaca
    15361 ttgcgtcgga gcttgtccgc gcctgttcgt aagtcaaaac accgacgcta cgtcggcgac
    15421 cgccgcacat ctttttgttc cgcccaggtc aaaagcggcg tctcttcagg atgaaacggc
    15481 agccacggcg tctttcccag cgtccgtcgc atcatcatca ccggatagag caaaaacgac
    15541 ggcggtcgcg caggtcgatt ttgccttttg gcccgatccc gctcgcgctg cgaaagtggc
    15601 agaaattcgc taactttcgg tgcgcgcagc gacgacaaaa gcccgtccag ttccgatctt
    15661 cgatgatcga ctatcgatgt ctccatcgaa aacgcgccgc actgtccgtg ccagtcggac
    15721 agcatttggc aggcgcccag cgcttgtcca cggtccgacg cgacaccgat tcgctgccag
    15781 ccttttttct tcgcaatccg cagcgagttg tagacgtttt cctcggtgtg cagcgcgtgc
    15841 ggttcaagat aaatccgatc cgccggcacc cccatcgccg tcatcgccac cgccagcgct
    15901 tccgcctcga caaatggggt gtagaccgct gatcccgacg tgatgaagtg ttgtgcctga
    15961 ccgcgttccc acagcagcgc cgcccacacc gcccgccttt gcagacagcg tgacagtgca
    16021 ccgtcggcca gcgtcggaca gccgggaatg atcagcgcat cgaaaggctg ctcgacgggg
    16081 gcacgtactt ccaggtggcc cgcgcaaccg gccatcacga cggacatcag catcgtcccg
    16141 aacataacac agcaaagcca cagtccggcc cgccgaaagc ccgagcgcgt tggcagacgg
    16201 tgtgatgcag tgcccatgac aagttagcct cccagatcac tgattagagg ctgtagctcg
    16261 tcatgaagct tcatccgaag ccgtcccgca agctggctgg cttcccgcag cgccgcccgg
    16321 cccggtccca gcttccgtct ttcgacgagc agcttgccaa atgccgccag tccctgcgcc
    16381 agttcaatct gatcgccacc ttcgcgcaga agcgccaccg cccgcttgag ataatcccag
    16441 gccgggtgct caccctcgac ggtttcatca aacagcgtcg aagcatgaat ttccccgagc
    16501 gtcaaaagac cacgcgcaat caaactgcgc agccccgact gctgcgcccg ctcaagcgcc
    16561 cgctgacaca gctccagcgc tttttgtttg tcaccgcgtc gcagatgaat cagcccgagc
    16621 agccgcttgc tttcggccag accacgctga tcgagcagcc gcagcgccag ctctttcgct
    16681 tgcaaaagcc gcgactccgc ttcgctcaag agcccatcgc gcagcatcgc atcgccgaga
    16741 cagcagagca gcgtgaccag ttctttcgac ggacccgcgc gttcacacag cggcagcgcg
    16801 tcttcgagcc gtgcccgcgc cgccgccact tcgccgcgcg ccagatcgac gctgccaagt
    16861 gccgtcatcg ccgccgcttg cagccgaggt tcgtcttccg acttgcgcag cgcttcttcg
    16921 taacagctca ccgccgcatc gagcgcccca cgctggtatt cgaccaaacc aacatgcaaa
    16981 agcgactgtg cgactttttt gcgatcgagc agcctgcgcc gcatttccag cgactgcgcg
    17041 agtcgatcca tgccgtcgga ctgcttgccc agccagacca gcgcttggcc ttcctcgtcg
    17101 agcgcatcgg aaatcccgac ctgatcttgc agatgcgtaa acagctccag cgagcgctga
    17161 atgtagcgta gcgcgcgcga cggatcgtgg gcgacgatgc gcgccatttc caaaaacgcc
    17221 tgtgcccgtt ctttgcgcga cgccagcgca aaacccagcc gcaccacgtt ttcccacgcc
    17281 gacagcgctg cttccaaatc acccagcagc gaaagcacgc gcccgacctg ctgccacagc
    17341 cgcagccgcg tcgacaaatg ttctgacgac agacacgcaa gtccgcgcaa aagcagccgc
    17401 ggcgctcgtc ccgtcgcacc gctcttgact gacagctcgg cggctcgttc aaacgaaagc
    17461 gctgccaaaa gaccttcgcc cgcccgttcg taatgacgcg caatccgctg ttgcaggtct
    17521 tcccggtcag cgtcgggctg cagcagcaga aactgcgcca tcagcaggtg atagcgacga
    17581 cgacgctcgg attcgacctg ctcgtaaatg gttttttgcc acggcggata ggcaaaccgc
    17641 agctcttttt cgacgccgag ctggctgttt ggaaccagca tcagcacgcc ctggccaatc
    17701 agctgctcgc acagctctga cagttcagcg acgcgctcgg ggtccccgtc ttggatgtcg
    17761 gcaagcggcg ggccgtttag gtccccgtcg cccagctccg tcagtccata cgcctcacag
    17821 cggctcagcg tcagaagcgc gccaagatgg aagttttcgc ccgcgaccgc cgcgtattcg
    17881 agcagccgac gcgagccttc tgccatcgcc cgcaggcgcg cctcgacaac ccccggcagc
    17941 gacgacggaa gctccacttg actgagcttt tcttcgtccc acaccggcac aagcgagcga
    18001 tccccatcac ccggctgcca cgacagcacc tgggtttcga cgagcaaacg caaaaattcg
    18061 accatcgccc gtggtgtgct ttcggaaaac tgctggcagt gcgagaccac ccacgacggc
    18121 aattcctcgg gttcggcacc gacgattgat ccgatgagct gcagtttttc ttcgtcgcag
    18181 agcagattca gctgcagccg cacaggcgga ctgctcgtcg caccaaagtc ggggaatgtt
    18241 tccaaaaact cgggccgcgc cgccagtccg acgaccaccg gcaagtccga cagcccgtcg
    18301 agcagatacg tcaccaggtt gaccgtttcg gggctcgcct ggtccatgtc atcgagcaag
    18361 atcaaaagcg gcccgcgtcg cgcatccgcg acaaagaacc gtttgatcgc cgaatacatc
    18421 cgcgactcgc tgcgcacgct ggccgcctgc gaattcgtcg gctgggccga tccatcggga
    18481 aacgggatgc ccaaaagctc gccaagcaca tgcgccactt cggtcgcgcg caccgccggc
    18541 aaaagcacac gacacagccg caaaagcttg tcccgagcag cgccaatcgg ttcgcttttt
    18601 tgcagatcgc agcgacgacg aatcagctcg atgaacaccg ccagcggtgc cgcaccttcg
    18661 ccgggtaccg tcgcttgcaa cacccgatcg ggcgacaccg caaaatacga cgtggccgcc
    18721 cgtcccagct ctgacagaag ccgcgtcttg ccctgccccg tcccggcgat aagcagaaag
    18781 ctgcgcaggc tgtgcgactt gatgctctgc tcgatgcagc cgagcagccg gcccagctcc
    18841 tcttttcgtc cgcaaaacgg cacctcggtg tcggcggatt cggcctgcaa aaggtcttct
    18901 tccgacaggg tgtaatgcgg cccggtttca tccccagaca gcatctcccc gtcgagccgc
    18961 ggaaccagcg ctgtgcggca gcttccacag atttcggttc cgtcgggcac aggtgcgtga
    19021 cagctgaggc agcgagacat cgtccgcttt taacgcgagt gcagaagctt gcacaagacc
    19081 gcaggcagcc caggctggcg aaaagcgccg cacgtcgcaa agagccgcga tattgtgaaa
    19141 aaacgatgag cgaggaacgc attccggagc cacggcgctc gcgactgtcc acctacaacc
    19201 aggttgcact ggctgccgca gcgacggtgg cgctctgcta tttcggtcgt ttggtgatca
    19261 tgccgacgct gatgggggtc gcgtttgcgc ttttgctcgg tccttttgtc gacgcctgtg
    19321 cgcgcgtgcg gattccgcgg tcgctgagtg cgctcttgct catcacgctg acgctgacag
    19381 tgatgtatgc ggcgacgcac cttctgtacg acttggtgac ggagtttctc gacgaactgc
    19441 cgagacattc ggcgaaattt gacgcttttt tcgacgcgat tcgcaaaaag ctcgacagcc
    19501 tcgccaaaac cactcggctg ctcggcaaca cgcccgaacc gagcatttcg gtgcggcagc
    19561 cgatcgactg gcccaagctg gtcattggtg gcgcaaactc tctcggtgag tttctgttta
    19621 tcacctcgtt tgtgccgttt attacgtact ttttgctgac gtggcaaaac cacctctacc
    19681 gggcgacggt gcggatgttt tccgtcgacc gccgcagcga agtcgagcgc acgatgggac
    19741 aaatcgcatc catgatgcgc agcgttctgg tcggcaatct gctcgtcggt ctgctcatcg
    19801 gcgtgctcag caccgtgctg tttgcgctgg tgcatgtgcc ctacttttac atcctcggct
    19861 tcgtgagcgg cctgctcagc atggtgccgt atctgggatc ggtgctcgcg gtgctgccgc
    19921 cgctccttat ggggcttgga cagctgagtg gatcgggggt gctggcgctc ggaatgggcg
    19981 tgctcttgct ccatcttttg gcgataaacc ttttggtgcc caaaatgatc ggcaagcgag
    20041 tgcagataaa tccactcgcc gcgacgctgt cgctgctgct gtggggggtt ttgtggggtg
    20101 ggattgggct gttgctcgcg gtgccgattg ccgctgccat caagatcgtc tgcgatcaca
    20161 tcgaaccgct gcacgccatt ggcgcgtggc ttggcgatga tctcgacgaa gatcctgcgc
    20221 ccaaagcttc cgccgcggag cacgcttgac agccgctttt cctcgccaaa gcttatccaa
    20281 catgcgccga aaaaacgctg cgtccgtgtt ttccactgcc ctttttgcgc tggccctgat
    20341 ctcgctgccg gcctgtcagg gctccggtcg tcaaaaaacc gtggctgcca aaaaagcagc
    20401 gccgacgaca gcgccaagtc cccccgcgtc gccgtcgggt ttgcctgccc tgccaccacc
    20461 ggcaacgacc agtcgcacag gcggcattga gcttgtggga acgacccttc cagcggcgct
    20521 caccccaagt cagcagagcc gtcgcacccc cgtcgtcgat gcggtgaacc gagcggcccc
    20581 atcggtggtg tcgattttta gcgaacaaaa accccagtac aatccgtttg gctttttcgg
    20641 tcttgaagac gatgacgaaa cagggcgcac ctcgctcggg tccggtgtca tcgtcgatcc
    20701 acgcggcttg atcgtcacaa acgagcacgt cgtcgccgga gccgccaaaa ttcgcgtcct
    20761 gctcgccgat ggccgcgagc taccagcaac actcgtcggg gccgatcagt cctttgactt
    20821 ggcggtgctc aaaatcgaag ccgtcggcaa agacaacaaa gaacccaaag acctgccgtc
    20881 gctcaagctc ggaacggcgt cggaccttat gatcggcgaa acggtcatcg cgatcggcaa
    20941 tccgttcgga ctttcgcaca ccgtcacgtc gggcgtgatt tcggcgctgc atcggatggt
    21001 gcgcgccaaa aatcgcacct acgaagactt catccagatc gacgcgcaga ttaaccccgg
    21061 caactcgggc ggcccgctgc taaacgtaaa cggcgagctt atcggcatca acaccgccgt
    21121 ccactcgggc ggtccgggca ttggctttgc gatccccatc gaccgcgccc gcaccatcgt
    21181 caacgatctg ctccagtttg gccgcgttcg ctatggtttc gtcggggtcc gcgtcgcccc
    21241 catccacgag cgtcgtcgcc aagccaccgg cgtccttgtc accgaagtcg atgcgggcag
    21301 ccccgccgaa aaagccggtc ttcagcccgg cgacgtcatc acccacatcg gcgacgaagc
    21361 caccgtcagt gtcgagcgtt tttgggaccg cgtccaccgc atcctcgccg gagaagacgt
    21421 ccgcgtgcgc ctgacccgcg gcgaagtcag cgtccacacc acggcgctcg atccacgcga
    21481 gatgtcacag cgcgcaaaga aacgactcgg gctcgatgtc atcgacgccc aaggccgtgc
    21541 cgtgctcgtc caaagggtca gtcaaggctc gatcgccgat cgcatcgggt ttcagcccgg
    21601 cgacgccatc ttgcaaatcg gagcccgcca gatccgctcg caaaacgact tcgtcacggc
    21661 acttggcgaa attcgacccg gcacggacac cgtcatgctc gtgttccggg gtcagacttc
    21721 gtactacgtc accgttccgt tctgatcgct tccgcctcgc ttcaggggcc ctgacttccc
    21781 ttgtgtcccg ccggcgtggg atgatgagcg gcgcgcagtg gggcttcgat gcttcgttag
    21841 atcgccaggc catgccgctg cgtttgccgg ggaagcttgg agatgcacat gcgatcgaaa
    21901 cgacccacaa aagccctacc cgcgatcaca gccagcctgc tgctgtctgc caccatcgct
    21961 tgcgatccac cgaagccgcc caaacctgcg caggtggcaa cacccacgac accgcagcca
    22021 ccaccggtcg aagcgccccc gctggcgagc gacaaagtgc tggccaagtc gccgcttgaa
    22081 gccccaccgc cgcccgaaac cgcaccgccc gaatctggtc ccgccggtcc caaaccaccg
    22141 ctgccgcctg tccccgccga gctggcgccg cccgcatcgc cgcgactttc gggtgtcagt
    22201 gcctccgacg tcgtaaagac caaggtcgaa agctttttcg ccggcaaaaa cagccgccgc
    22261 atccacatcc agaccgacaa gccgctctac aaacctggcg aaaccgtctg ggttcgcgtc
    22321 tgggacgtca ccaccaaagc gctttctggc aaacacccag gcgccggaat gcacgtcgag
    22381 cttatcaacc cgcgcggcgc ccaaatcgcc aaaaagaaag tccgcgaagc cggcggctcg
    22441 ggccagatcg actttgctct cgacggcgga atcgtcggtg gtgaatacaa gatcaaggtc
    22501 aagacgttcg acggtgtgca ggacgagcgg cctctcatcg tttcgacgta tgagccgccc
    22561 aagctcaagc tcaagctcga gtttgtccgc aaagcctatg gaccgggcga cgaagtgacg
    22621 gcgacgattg ccgtcaagcg caacaccggc gagccgctgc gcaaccacgg gctcacggcg
    22681 acggtgatgc tggatggcca agcgctgccg caggttcagc tgcgcaccga cgaagaagga
    22741 gacggtctgg ttcgcttcaa cctgccaaat gaaatcagcc tcggcgacgg tcttttgacc
    22801 gtgatggctg acgacggtgg catcaccgaa tctatcgcca agcgagtgcc gatcatcgta
    22861 aagaagctca acttggcgct ttatcccgag ggtggcgacc tggtctacgg gctgccggga
    22921 cgggtgtatt tcgaagccaa aaatccgatc ggcaagccgg cggacatctc gggtcgcatc
    22981 ctcgatgaaa acgaacagac cgtcggatca tttgaaagcg tccgcgacgg cctgggacga
    23041 gtcgacttta ccccgcagca gggccaccgc tacaccgtca ctgtcgatca gccggtcggg
    23101 atcaccgatc ggtttgtcat gccgacgccg caggaacaag gctgtgtgat ccgcagcatc
    23161 gacgacctcg acggacaaca gacggccacg cgcgtcagcg ttcgctgcac cgacaaacgc
    23221 aaagtcgtgg tcgccgcgac gatgcgcgaa aacctgctgg atgtcgccgc ggtggaagcc
    23281 gatcgcgaac cagcgacggt gtatcttgaa ccacgcggcc agggcagcga agcgctcgga
    23341 cggacacaag gagccgtgcg ggtgacggtg ttcgacgaag cgtaccaacc gctggcggaa
    23401 cgactggtct atcgacaaag acgggcacga ctgcaagtgc aggtggaccc gatcaaaaag
    23461 agctttgttc cgcgcgaaaa ggtcacgctc gacatcacga cgcgcgacgt gcaaggcaac
    23521 ccggtaccag ccgatgtggc actgtcggtg gtcgacgaca cggtgcttag ttttgccgac
    23581 gacaagaccg gccacatgct gagccgcctg tatcttgagc ccgagcttac cggcaaagtc
    23641 gaagagccga atttttactt cgacctgtcg gaaaaaaaga gcggcatggc gctcgatctg
    23701 ctgatgggca cgcgcggcta ccgccggttc gagtggcgcc cggtgctggg tggtctgatc
    23761 gcccaaaaca ccggcaaagc gaaagacgag acggcgcaga cggtgacaag cttttccgat
    23821 ctgcgcaaag ccagccgtgc gcttcccaag cccgacaagc cgcaagacgt ggtgctggcg
    23881 cagaccaagg caccgccgcc ccctcccgag cgcaaagccc acgctgccgc cgagcgtgaa
    23941 cagttggtgg tcgcggtgaa caagccgatg gcgggtccag ccaaaacccc ggtcgccttg
    24001 ggaacggtgg ctgcgacgcc acctatgcgc gctggggcgg gaggtggcat ggtggccccg
    24061 atggcagcgc ccgcgatggc agccggggcc gccccgcgtc cgatggctgg gcacgcggac
    24121 aaggccgatc ggatggtggc gacggaagac tcgggcgcgc cgatgaaaga agaaaaggcg
    24181 atggccgccg ccgagccgat gatggccaaa aagaaaatgg cgatgggccg agccgcggcg
    24241 atggacgatc tggcgggcgg cgacgacatg atgggcggtc cggtgggctt cccgatgcgg
    24301 catcgcgact ttcagcggcc tcgtccgcgt ccggtcgagc catgggcgcc ggtccgcgtc
    24361 ttcccgcagc caacctatca gcctgactac gttggtccgc gcacggattt ccgagagacg
    24421 gtgttctggg ctccatcggt ccagaccggc caggacggca aagcccagct ttcgtttgtg
    24481 ctgtccgacg cagtgaccag cttccgggtg cagaccgaag cgatcggtgg cggtgccgca
    24541 ggtcgcgaag aaacggtgat caagtcgagc ctgccgttta gcatggcggt gaaactcccc
    24601 gtcgaggtca gcgaaggtga tcggattgcg ctgccgctga ttttgtcaaa cgaacgcgat
    24661 cgggcgcttc aagtgaacct tgcggcaagc ttcggcagct tgctcaagct ggaacgaccc
    24721 gtcgaccgcg cccaaggttc gattgcagcc ctgtcgcgcg attcgctgtt ttatccgctg
    24781 gccgtcactg gcaaaaaggg caaaactgaa gttcgtttcg tcgccgatgc cggcggtctg
    24841 cgggatgagt ttgtccgcga aatcgccgtc gcaccgcgcg gttttccgca gaacatctcg
    24901 aagtcgggtc ggctcaaaga caacgtcgtg cacgaagtcg agctggcggg acttatggac
    24961 gggaccagcg acgcgcactt gaaaatctac gtcagccaag tctcgacgct gattgccggc
    25021 ctcgatggca tgctgagcga gccgggcggc tgcttcgagc aagcctcgag caccaactac
    25081 ccgaacgtga tgatcctgcg ctacttgaag acggcccaga tcgcggcccc acgggtgatg
    25141 tcgcgcgcca gcggactgct cggggacggt tacaagctgt tgaccggata tgagacgccg
    25201 accaaaggct acgagtggtt tggttcgacg ccgggccacg aagcgctgac ggcttacggc
    25261 ctggtcgaat ttaccgacat gaagcaggtg ttccccgaag tcagcggcgc gatgatcaag
    25321 cgaaccgccg attggctgca caaccgccgc gatggcaaag gcggctactc gcgcaatccg
    25381 caggcgctcg acagctttgg atcggccagc aaggccgtca ccgacgccta catcacgtac
    25441 agtgtaagcg aagcgaagca gcccggcttt gaacgcgaga tcgatgccca agagcaagtc
    25501 gcgcgcgaca cccaagacgg ctacgtgctg gcgcttagca caaacacgct gctaaacgcc
    25561 ggtcgcaaag acggtgggta tgcggcggcc aaacgactcg ttgcgatgca agatccgtcg
    25621 ggggcctgga aaaacgccag ccacagcatc acccgatcag gcggacgcga tctgcaaatc
    25681 gagacgacgg cgctcgcgat tttggccctg ctcaagagcg gacagttcga cgaatcggtc
    25741 gaaaaaggca tggaatggct gatgcagcac aggtcgggtc acggcgcgtt tggcgcgacg
    25801 caggcgacgg tgctggcgct caaagcgatc actgcgtact cgtcagcgcg agcgcgagcg
    25861 atgacgccag ggatggtgac gctggtcgta aacggcaaag aagccggaca gcttagctat
    25921 cagcccggcg atcgcgagcc gcttacattc gcgggcctgc aaaaacactt ggtcgccggc
    25981 aaaaaccgca tcgagctgcg tcacacggga cagacgccgc tgccctacag cctcggggtg
    26041 agctatcgtt cggaccagcc agcgacgagc ccagatgcga aggtgtcgct tgttacggcg
    26101 ctccagcgca atcaggtaaa gctcggggaa tcggtgcggc tcgacgtgac gctgaccaac
    26161 aagaccgatc aaggcctgcc gatgacgctg gctcgagtcg aaattccggg tgggctccag
    26221 tttcagacct ggcagctcaa agagctgcgc gaaaaagggc tgatcggctt ttatgaaacg
    26281 cgcccgcgcc aggtgaactt gtacctacgg cagatgcagc caaaacagga agtgaagctg
    26341 ccgctcgact tgattgcgac ggtgccgggt gactatacgg cgcaggcgtc gacggcgtat
    26401 ctgtactaca caaacgagca caagacctgg gtgccgggca cgcgcatcgc catcgagtag
    26461 cccccgaaag ctgcgcagcg gttacttgac gatgatccgc cccggcatcg cgcgaccgtg
    26521 aatcgcgcag ccgtaatcga acgtccccgc ctgattgaac gtgttttcaa acgtccccat
    26581 cgacatcgtg tcgctgccag gaaaccccat gtcccacgga aagacaccgt gctgcgacag
    26641 cgcgcggaag acccagcgaa cctttccacc aacgggaatc gtcacttcct gcggtgaata
    26701 gtaatcgtcc tgcacggtga tggtcgccga cggtgccaaa tccatgatcg gcgcccgcat
    26761 gtcgaccacc gccgcaggag gcgtgtccgt cgtcgtatcg gtacagctca gcgcgagcac
    26821 acccacaagc gacagcccca aagaccccaa aacccagagc acaccatttt tatgcatgtt
    26881 agaagcgtat taaatgggca gctcgctggg aagtcgcttg cgtgctctga cggtgacacc
    26941 acccatcgtt tcggcctggg caaagtcgga tccttcgtac cactgccacg gcagcgccgc
    27001 gactttgggc aggtaacgac ggacgatgtt ggccgtgaaa cctggcagat agcgcatccc
    27061 gaaaccgaac atcgcaccaa accagcgatc cattcgatcg tggcgcggac agactttttc
    27121 gatcacgaag ccggcgcggt aaagccgctc ttgcagctcg ctgccgcgat agcgtcgctc
    27181 atggcccatg actttgtcgt agtacgtcca atagtcgggg tttaccggac acgaaaaaaa
    27241 caggtggccg cccggcgtca tcacccggca aatctcacgc aaaagcgctt catcttcggc
    27301 gacgtgctct agcacttcaa acagacacac caggtcaaag cgcgcatcgg catacggcag
    27361 cggcgtcgtc gcaacagcac agtgcccacc gcgatcggcc agtgcccgca gcgccacccg
    27421 cgacaaatcg acaaagtgcg tcccctcgat cggaacgtgc ggaaacatcc ccggaccgat
    27481 ctcgagcgtc cgatccggcg catacagcgg cgcaatttcc gtccaggtgt acgtccagtc
    27541 gctgtgcgga aacagctcgt agtgggtcca aagatcctcg tagatcgcgt ggtttctctc
    27601 ggcggcggcg ttcatgcgca gaagctagcc cggcccccat ccccagacaa gagccaccta
    27661 tgacccgcaa agtcgcgata cgatgtgact ttggcgagtt ctgtctgatc gctcgccggg
    27721 gggacggggg ttatgagcag acagggattt atccttcagg cgagcatgcg ccttatggcc
    27781 gggcagccgg tgctgctgct ctatggacgg ctcgcggaag gcgagacttt tttggtaaga
    27841 gacgatcgcc agcgcgcctg tttttttgtc cgcacttgcg acggggaagc ggtgcaaaag
    27901 ctggctgggc cgaccgtcaa gctgctcaag acgccgcggc aatcgctcgc caaagagccg
    27961 gtgtcacggg tcgagctaca gacgccggtg tcggcagcag cgctgtcgca tcggctccac
    28021 gcggcgggca tcgcgacgtt tgaagccgac ctcaacatgg tgatgcgcta tctcatcgat
    28081 cgtgggatac gcggcagtgt ggaaatccac ggcagtgcgc ggcgcggacg accgtccgaa
    28141 gaaggcgtcg atctggtgtt cgacaatccc gagcttatgc ccggatcggt gctgccaccg
    28201 ctcaaaccgc tgacgattcg cctgcaagaa ggttccgacg gcacactggc ggcagctgcg
    28261 atcgccgatg agctcagcac ggtggtactg caccggaaac cgacgacaag cagcgacgca
    28321 accgcatttc ccgatgaacg cgcgctgctc ggtgccttga tccagcggat cgccagcaaa
    28381 gatcctgatc tgctggtcgg ctggaatctt ttgaccgaag atttgccact tttgcgcgac
    28441 gcagcccgtc gctgtcgttt gtcgctacca ctcgcgcgcg gaccgggcga tctgcaatac
    28501 caaccgggcg tgggccgtgg tcagacgcca cgcgccgtgc tgtccggtcg ggtggttttc
    28561 gatgtggcgc cgatctggcg acatgtcaaa ggggccggct atgaagactc gctggcggcg
    28621 cttctcgacg gggaaaccgg caagcacgcg cccagcgaag tcgacacagc gaaccccgat
    28681 gaactgggac ggctccgtcg agaagccgaa ggactgcgcg atctgctcca ccgctacggt
    28741 ctgctcgact tggcgatcca gcgcagccgg ctgaccggtc tgcccatgga tcgggcgacg
    28801 gcgagcgttg ctgcgtttga ttttctgtac ctcgtcgagc tgggacggcg cggctatgta
    28861 gcgccgacct tcgagcgacc gaccgagctc ggcgagcatg tgatcggcgg acacatcatc
    28921 gcgccgcttc cggggctgca caaaaacgtc ttggtgttcg actttcgcag cctgtacccg
    28981 agcgtgattc ggacgtttca gatcgatccg ctgggtcttc tttccgacgg agacggcggg
    29041 gaaccagatc cgatcgtcgc gcccagtggt tccaagtttc gtcgccagcg tggcattttg
    29101 agcgtgattt tggatcagct tctgccgctg cgcagcgccg ccaaaagcca gggcaacgaa
    29161 gcgcaagccc aagcgatcaa gctgctgatg aactctcttt atggtgtgct cggcagccca
    29221 gcatgtcgtt tccacaaacc cgagctggcc aacgccgtca ccagcttcgg gcgcgagctg
    29281 ctccagtttt cgcaagcgcg actcgaaagc tacggacaca aggtgctcta cggcgacacc
    29341 gatagcttgt ttgcgacgac aaacctcgac gacagcgcaa ctgaagcgca gctgcgatcg
    29401 ctcggcgaaa cgctgacggc gcggctgaac gccgatctgt ccgattacat cgaaaaaacc
    29461 tggcgcgtcg aaagccggct cgagctggcg ctgcactcgg tctaccgtcg cttgctgcta
    29521 caccgtcagc gcggcaagga caaaggcgcc gccaaacgct acgccgggct gctgctcaaa
    29581 ggagatcagt gtcgactgca catcgtcggt ctggaagcag tgcgccgcga ctccagcgag
    29641 ctgtgccgca ccgtgcagca gacgctctat tcgatgctgt tcgcgagcgc cagcaaagaa
    29701 gaaatcgccg cctacctgca tcgcatcatc gccgagttgc tcgctggaaa gctcgacgcg
    29761 atgctggtgt atcgcaaagc gctgcgacga gaaccggctg actacgtggc aaaaggtgcc
    29821 ccccacgtcg tcgtcgcgac ccgtcacgga cttgcgcctg gcagcttggt tccctacgtc
    29881 atgacgacgg caggcgccga acatccagcg catcgccaaa gtccgatcga ctatcagcac
    29941 tatctcgaaa aacaggttcg ttccgtcgcg gaacccgtgc tggcgcacta cggcctgcca
    30001 tttggacaaa agcgccaaag cgatccgcag ctccgcttgc tctagatcac aggctttgac
    30061 caccgtcgac gggtagacac acgccggtca gaaacggctc acgcagaaga aacgccaccg
    30121 cgcgcgccac atcgagtggt gatccggcag gatatcccgc ccatttcgcc gacaaacgcg
    30181 caagctcgtc ctcgctcgcg tcgtcgggcg gaagaacaaa tcccggtagc acggcattta
    30241 cccgcacccg cccgatcagc tctttggccg cgatcttggt cagctgcaaa agccccgctt
    30301 tcgagacgcc atacgccgca aaaccgcgcc acgcttgcgt cgcaccgaca tcggccacgt
    30361 tgacgatcac ttttgccccc gcagcaatcc caagctgcaa gcagcgacgc ggcgccgtca
    30421 gattgacagc gatcagatca tcccacgccg catcatctag cgtttcaatc ggcgtgcgaa
    30481 caaacgcggc agcattacag acgacggcat cgattcgcaa gcactcggcc agcacgctcg
    30541 cgaacatatt ttccgtcgct gcccgatcac gcaaatccgc ctgaacaatc cgcgcacgcg
    30601 acccaagctg cgaagccagc gcctgcgcat cggccatcga ccggtggtag tgaatcacga
    30661 tcgacgcccc ttgctgccac agcgtctcgc agatcgcccg ccccagacgc cgcgcaccgc
    30721 ccgtcaccag caccacctgt ccagccagcg gctgcgcatc atctgtcgtc gagccaaacc
    30781 cctgtggcag ttttttctgc atgaccaaac cgggcgctat cgcagcgcga ggcgacctcg
    30841 gtccgcaaga aaaaacgcta aggaattggc caaagctccg cgtatagtac cggggcacct
    30901 atgttttccg tgacgttgtc ctcggcgcaa gcgacctcgg cggcgccatc cgtttcttca
    30961 accactgtgc ttgtcgcgct gctggttgtg gccatcgccg tgatcgcggt ggttctgctc
    31021 cgtcggaagc cccagcttcc cgaccagcca gataccaagt ctgcgggcga accgaacctt
    31081 ctgccgagtc gggccaccac tgcggcaccg cagccaagcg acaccgccgc cacgccgagc
    31141 cccaaaagca aagccggtca cgacgagcta aaggccggtc tttccaagac gcgcgctggt
    31201 tttattgccc ggatcgccga gcttctgtcg aaaaaacaaa tctcggacga tctgctgccg
    31261 cagctggaag aagtgcttct gacagcggac atcgggccca agaccgccga gcacctgttt
    31321 tcccaggttc gttcgacgct gtcgcgatca gagctcaaag atccggcggc agtctggggg
    31381 gtgataaaag acgaatcgcg gcggcttctg aacgtggatg cacccgcgat cgacatcgct
    31441 gcccatcagc cgtttgtcct gctggtcatc ggcgtcaacg gcgtcggcaa aaccaccacc
    31501 atcggcaagc ttgcggccca gcacatggcg caaggtcgca aagtgatcct tgcggcgggc
    31561 gataccttcc gcgccgctgc cgtcgagcag ctcgaaatct ggggtcaacg agccggcgtg
    31621 ccagtcgtgc gtggcaaaga aggcggcgat ccctcttcgg tgatttttga agcgatcaag
    31681 cgggcccagc gcgagcagat cgacttggtg atcgccgaca cagccggacg gctccacgtc
    31741 aaagaacagc tgatggctga acttcaaaaa gttcgccgcg tgataaaaaa agccgatccg
    31801 accgcgccgc atgagacctg gttggtgctc gatgcgaccc aaggtcagaa caccattgcc
    31861 caggcgcgaa cttttaaaga ggcgatggaa attaccggga tcgtgctgac caagctcgac
    31921 ggcaccgcca aaggcggcgt gattttgggc atcacccacg agctaggcgt cccggtccgt
    31981 tacatcggtg tcggcgaaaa aattggtgac ctgcgtgctt ttgacgcaga agattttgtc
    32041 gatgtgttat atgagcgttc tgcctaacgg cagaagcaaa tgggccgcgg tctgggccca
    32101 caagtaggct gcaaacgagc ccaagcagta ggtacgacag cgtgcggaac aacgagttcc
    32161 tcgtcgagag tcacgattca cagccctccc ccggccaacg gtccgtcggg gaggttgtgt
    32221 cgtttgtctc tagcccgcac gataccagcg acgacaatgg caatttcggg aagggcttct
    32281 cttcgcaaag acgaaatcag tcccgaaagc cgcttgattg cgacaaaagg cgcatgatat
    32341 cgtttcccct cgatatcaga ggatgccagc cccacaagg
//
LOCUS       DAORGN010000031        32353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_512_length_32353_cov_6.748932, whole genome shotgun sequence.
ACCESSION   DAORGN010000031 DAORGN010000000
VERSION     DAORGN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 32353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32353
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_32353_cov_6.748932"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKL17_05600"
     CDS             <1..880
                     /locus_tag="PKL17_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNK44235.1"
                     /translation="LSPNEVALLCTGTQGESVAALGKLVAGLSPDSAPPFGKLRLSAG
                     DCVVLAARAIPGNERAIARLADRLVAHRVEVVSGPHYSVSGHGYRDELKWLLQTFAPR
                     TLLPVHGGPRQLAAHAEIAHSLGIPALVQHNGDILELTDAGTVQVVGQLPSPGLAVEG
                     TTVGEVGQDTLRLRRQLAQTGLVIVAQVFGKPHRLAVRAVGISDPGPQLDALCQKAAR
                     LGEEVIAALRGTEPTPPDLHELERAVGRAVRGVFRVARGIKPMVQCVLCENSAGDELI
                     EAAAVGPSPQQMVDKD"
     gene            complement(824..1888)
                     /gene="aroC"
                     /locus_tag="PKL17_05605"
     CDS             complement(824..1888)
                     /gene="aroC"
                     /locus_tag="PKL17_05605"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNK44236.1"
                     /translation="MGASSFGQHFVVTTFGESHGAALGVVIDGCPAGIPFLPDLLQSE
                     LQRRRPGNKTATGESLTSARAEPDAAEVLSGVYAGKTLGTPIAMLVRNLDARSQDYAD
                     IARSPRPGHADDVWRHKFGHSDPRGGGRSSGRETVARVMAGAVAQMVLRQLAPDLVVR
                     GFARQIGPLSLSEDEVAALQADPPAVRQIDDFPARLPSLRLKTQVEQLLLAAKREGKS
                     YGGLVELWCDGVPKGLGQPVFHKLKSDLAAALLGVGAVSSVELGDGMLAAKAEGSEFH
                     RAADSPDRYGGIRGGISTGERIVLRAACKPTATVLDTAKAGRHDPCIVPRAIPVLEAM
                     VYLVLVDHLLWRRTDRGSFD"
     gene            complement(1891..3144)
                     /locus_tag="PKL17_05610"
     CDS             complement(1891..3144)
                     /locus_tag="PKL17_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44237.1"
                     /translation="MNSDRLPFSFSGTIPPSKSLLIRGLLLQLTEPAVTLPVLSRCSD
                     VVAMQGACAAFAAGQTRIHCGEAGLVLRLCLGYTARRGGDFVLCGSRRLIERPHEPFL
                     SVLRQLGAQVSICDDGAAPALRTRSLGFADPGGRLSLLSGLSSQFASSLILNAWDLPF
                     PLHILCEKEPISEGYLDLSIDVAQRCGMQIERQYEADKMILIVPPRQRLVPAVLPCEA
                     DVSSAFAVAALAAVAGTCHIVNFPLHSLQPDHCFVDILQKMGVSLDLSTKGLVVQRAE
                     RLRAIEVDVGGAPDLTPVLSVLCALAEGTSRLFGAPHLRGKESDRIATTSALLQVLGR
                     EHTPREDGIEIVGRPLADSDRSTLRQFDAGSDHRLVMAAALARWAGFPVEIAGLSAVH
                     KSFPEFLTIAGLADLAADTLSKKDS"
     gene            complement(3141..4724)
                     /locus_tag="PKL17_05615"
     CDS             complement(3141..4724)
                     /locus_tag="PKL17_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNK44238.1"
                     /translation="MLILSGPPGVGKTSIGRICAELLGLPFVDSDAVLSAQAGMPLAE
                     LIFRDGEAALRERELAFVNALDSAPCVLSVGGGLVTSPQVRARLRKLGFVCGLDASID
                     VLLARLAQSPALRPLLLPDPKKTLPDLLVRRESAYRDVDVRLSVDALSAQQAAAAVVS
                     LYRQLFVQKESFKILHNGPTSVHCADALPTLDDVGDAPLLVYDQRIEQLAQHADPALL
                     SWLHGFPQRYGVCAGEDLKDLAAFPKHVEHLLEIAAPLPQSRLSVVALGGGSVGDFAG
                     FFASVFKRGVPLQQIPSTWLAAIDSAHGGKNALNVGRAKNQIGTIVAPTHVWITKRVL
                     DLQPEERAHEAMGELAKIAYLDGGEWVQGLLASQKTGAALLWEWLPAAISAKRRIVEL
                     DPQERLGIRHKLNLGHTVGHVLETVHGLPHGLAVAQGLYFALSFSTQKGLLQATVKAD
                     LEARLRDRFGLYDRRSELPRIPQAQFVRLLAQDKKRSSKDEVRFVFLRAMGQSEVIPV
                     TLSELTQAAIEMGYVEPQS"
     gene            4775..6259
                     /locus_tag="PKL17_05620"
     CDS             4775..6259
                     /locus_tag="PKL17_05620"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44239.1"
                     /translation="MDEKQYAQSHRGATLTVQIQPGQSPQDVLRTVQKSITEGLRYVE
                     LRDDLICDEELLWISDRLPRPTKLLSLRRAREEERSRLSLIRRCQPALLDVALELGHD
                     ANRLYSLIETTGSVRGVVSCHARDRSVPFAQALANLSKQVPSHFATKAALPIDNLAEL
                     FDGHRWHLQDPQRNLFLPIASDGSGRFRFYRLLLGESLLLNFLRDPCAPLIPDQPTLS
                     EWQTRHHVPIPTQGPIPFAAIVGDPVEHSRTPAHHGSFFARYGMPVLKVRLTSLDLSS
                     DVLSILLSLGLRACAITSPHKTWLRDSIDQHSGMWEVSDDRDPQSAGNTLVACDDGTV
                     FGTSTDGIGLRAAWEETLIAQRERCGQNPGIAVFGGGGLLPLLRTVFPGALFLSARSG
                     KPRDASPSGPADKDITVLVWSVGPSRFVHNPPSGLRPKLVFDLNYAEDSPARAYAQSI
                     GAQHVDGSLFFRKQADAQQLFWQRHLGHSQAARDHVDFAKRSPQ"
     gene            6256..6693
                     /locus_tag="PKL17_05625"
     CDS             6256..6693
                     /locus_tag="PKL17_05625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNK44240.1"
                     /translation="MSAVLYSHPMIVPFQDIDAAGIVFFARIFDYFHNAFVEHLRARG
                     VSLPEVIAGGEWGAPLAHAEADYKFPLRFGDEVRVELVSADLGDTSVTLHFRIVSGSD
                     PVRVHSFGKTVHVTIDRKTFRPRPVPPALRAAYKATAPSDQSA"
     gene            complement(6620..8062)
                     /gene="menE"
                     /locus_tag="PKL17_05630"
     CDS             complement(6620..8062)
                     /gene="menE"
                     /locus_tag="PKL17_05630"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HNK44241.1"
                     /translation="MDVGLRTAAQQAPHRLALIHPDGAYTYSDLAERVFSVCAALDQR
                     GIPRKDVVAIRASNRWQTVVLLLALIERGTPFVPIHPRYTDHELSFVQKDAAVDRVFA
                     DSDVDSLIDASSTGLSSHAVISDEETLAILYSSGTTGTPKGAILSRSAFWASAKGSAE
                     NLGFCEDDRWLVCLPLCHIGGLSIVTRCVLARACMVLLPRFSAASVLDAIEAHRVTLL
                     SVVPTMLHALLEADERGILSRLRAVLCGGAATPFPLLSRAVDRGVNVLCTYGLTEACS
                     QVTVQKWLPKPLARRGSGTPLQGVEVAIRGEAGSFLPSGQVGQIVVKGRSVMTGYLHR
                     PPLAGDWLDTGDLGELDDSGTLFVHARRTDLIVTGGENVYPAEVEQALLTIPQVRDAL
                     VFGIPDEVWGALVCAALVVEKDFDQKQMESALAERLAKFKRPRRFVVVDSFPELPNGK
                     RDRLRAITRFGPMVQSPCTLRAGQGEPGAV"
     gene            complement(8053..9126)
                     /locus_tag="PKL17_05635"
     CDS             complement(8053..9126)
                     /locus_tag="PKL17_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNK44242.1"
                     /translation="MMGIAALSLPSPIVASRLHLVGGSLSGSFANSQRTFLDREAVVL
                     QLVDADGQTGVGEAAPLVGHSQECLSQCMSALHGVHKRITMDAEGWPVADGLSALPAA
                     RFALETAYLDLLARRHAVSIATLFQGLAQPIPRSAVAQSLTEAESAWQRGIRTIKVKV
                     GDPKKEASDELHILSQLRSRLGKSLRLRLDANGAWPLEKARAQLSCYAEWEPEFVEQP
                     VAVQELDRLGSCAVPWAADESLLHPIEAEKLLQSRDCSVFVLKPALLGFRCARQLALA
                     AAACGKGVVITHSLDGPIGLAAACELALSLPSAPLACGLDAHPGLSAFPAVAIPHHAE
                     SAAQIAPSRGPGLGFPVAGLPWT"
     gene            complement(9133..10053)
                     /locus_tag="PKL17_05640"
     CDS             complement(9133..10053)
                     /locus_tag="PKL17_05640"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNK44243.1"
                     /translation="MSRQRAAQRIPEGVQKNSVKAWILATRPATLTVGLIPVLVGCAI
                     ARSMHALRICPALAALFGAIFIQIGTNLANDVFDYEKGADTAERLGPLRVTQAGLIAP
                     HNVRLGMYISFALATVLGLYLTWSAGPVILIIGLLSIASGIAYTGGPYPLGYNGLGDL
                     FVFLFFGFVAVCGTVFVQAGSVPALAVWASIPVGAIATAVLVVNNLRDGATDVVAGKR
                     TLVVRFGRSFGVSEYVGLVMLAYVVPVLLMMTGQCGRAVLLPLMTLPLALPLVRSVSQ
                     DSGAQLNQTLAKTAQLLLFHGILFAIGLAQ"
     gene            complement(10075..10902)
                     /gene="menB"
                     /locus_tag="PKL17_05645"
     CDS             complement(10075..10902)
                     /gene="menB"
                     /locus_tag="PKL17_05645"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: protein motif:HMM:NF005637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNK44244.1"
                     /translation="MSDTTWKTVDLGFQDVKYEKTDDGIAKITINRPEVRNAFRPRTV
                     KELSAAFTDARDDADVGVVILTGEGKEAFCSGGDQRVRGQGGYVGEDGIPRLNVLDVQ
                     RQIRTLPKPVIAMVAGYAIGGGHVLHMLCDLTIAADNARFGQTGPKVGSFDGGYGASY
                     MARIVGQKKAREIWFLCRQYDAQQALSMGLVNTVVPVDKLEEETLKWAREMLANSPTA
                     LRFLKAALNADCDGQAGLQELAGSATLLYYLTEEGREGKEAFLEKRKPNFRKFKRFP"
     gene            complement(10899..13430)
                     /gene="menD"
                     /locus_tag="PKL17_05650"
     CDS             complement(10899..13430)
                     /gene="menD"
                     /locus_tag="PKL17_05650"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNK44245.1"
                     /translation="MIGKSLHTAWAELFLSSLRRAGVTDVVVSPGSRSTPLALAAAVD
                     PHLRVQVILDERVAAFFALGQARQSGRPSVLVCTSGTAPAHYLPAVIEASQSHIPLLL
                     VTADRPWEDYDCGAAQTVDQVKLFGDYVRHYAELGLPDPSGAALRAVVRVAGQAVSRT
                     LYPLPGPVHVNARFRKPLEPVFTEDAAAGIPAALLEELKESPAATAIVSDLLPSQAAV
                     ELLVTLCKDKRRGIIICGPAHVHTSCEALRVLLQRLSVQTGFPIFAESTSGVRFGIRA
                     EVCGGFDAFLRDAELTDQKPELLVEIGGFPVSSSYASFASRWADCRRVVIAPHGVQDP
                     IGTAQHVNADPVALLAKVCDLLPAQCPDTGWHSLFAQAEKRHWEVVQAELSDPILSEG
                     AVAHGLCLSLPDESVLLVGNSLPVRDLDLFCPPSEKPLRVLHQRGASGIDGLIATAAG
                     VKSQTSKTVALLLGDLSAQHDLGGLAPLAQASGPLVVVIVQNGGGRIFEQLPVGSSSA
                     AAPHFAKLFLTPQTIDFAQAAAAFGIPFVRAASTQSYQAALAQGIESERPLIIEAVVP
                     GDDGLARRKRIWKRSPQPSSQKARSEAQPWVFLHGFLGSPALWTEPTESLSIPSLIDH
                     LPGHGPSPWTLPDADFLAVIDQIADRIKPPRFGLCGYSMGARLALALAIRHPQRVSSL
                     MLIGVDPGIVDDAERQARIQWEDRWVRLLLEKGLPSFVSEWEKLPLFASQNRLSQTSQ
                     LQQRLSRLQHRPDAILWALRTLGTGRMPNLWPHVPSLQMPISLVTGEVDEKFSTIAQK
                     LTSIHPFIEHHVISGSGHNPILEAPAQMLAVFSSHLTRSFGESQR"
     gene            complement(13427..14815)
                     /locus_tag="PKL17_05655"
     CDS             complement(13427..14815)
                     /locus_tag="PKL17_05655"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HNK44246.1"
                     /translation="MRTLLARAKKASVGMAEDTLLCLSAPLSLSSPVRALVDSFQSTL
                     TSYYAPPARVADFACISIGQAVRIDQDGGLAISQVKARAAQIETQLVCLRAEACDAAP
                     QPRLFGGFRFAPDATRQSADDPWHAFADASFVLPRWTVFVREHESYVQLVIRPAELSA
                     AERLLEELDRIEHAAQGSSGNPSPVCGGDFVQTEQPSQAEYCAMVAAALAEIHAGVLQ
                     KVVPARRLQIDSSALFSLSQTLARLDDSYSDCCRFAICRDGSHMVGATPELLFHKRGQ
                     TLRTEALAGTRARRAELDDKQLVRELMSDDKEQREHAHVISAIASELHPHCESDPQVG
                     QTTIRTLRNVHHLCTPIAARLKQSTHLLDLVAALHPTPAVGGLPRDRAAQFLSTQEPF
                     SRGLYAAPVGYFDLAGDGAFWVAIRSALICGSRAWLYAGAGVVAGSIAQKEWAETNAK
                     CSAMRSALGVTI"
     gene            complement(14819..15553)
                     /gene="ubiE"
                     /locus_tag="PKL17_05660"
     CDS             complement(14819..15553)
                     /gene="ubiE"
                     /locus_tag="PKL17_05660"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNK44247.1"
                     /translation="MSETQPVKQDSSAAAGSGQMFDGIAARYDLLNRIISLGIDQRWR
                     KKTVAALELASGDRVLDLATGTADLAILVAQTAPDVDVIGVDPSRNMLAVGQRKIEQK
                     QLASRITLQEGDAQALPFAADSVSRICMAFGIRNVPDRALALREMARVTRKDGRIAIL
                     ELAEPREGLLSGIARFHVHSVVPWVGGLLSGSKEYRYLQQSIAAFPAASDFAALMESC
                     GLSVLSVEPLTFGVATLYVATPRKEK"
     gene            complement(15550..16533)
                     /gene="aroF"
                     /locus_tag="PKL17_05665"
     CDS             complement(15550..16533)
                     /gene="aroF"
                     /locus_tag="PKL17_05665"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNK44248.1"
                     /translation="MIVSLLKDADPQAVKRELTALGLWIASVEQGLGSGGIQFVVAPH
                     STVVDPQLVAKIRGVFAVSMPVPEHPRIDAQGPTVEVAGHAIGGRLPTLMCGPCSVES
                     KEQIDAIAARLAPLGVTFLRGGAFKPRSSPYNFQGHGDPALGWMRDAATKNGLRVVTE
                     VLSEQDVSLVAEQADLIQIGSRNMQNFALLKKVGKAGKPVLLKRAMSATVEEWLLAAE
                     YLLHHGASGVVLCERGIRGFDAATRNLVDLTAVALLANVHKLPVIVDPSHGTGRRDLI
                     LPLSRAALAAGAAGVMIETHDDPGRALSDGPQALRGDELEDVIGKLQAVTR"
     gene            complement(16543..17601)
                     /locus_tag="PKL17_05670"
     CDS             complement(16543..17601)
                     /locus_tag="PKL17_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNK44249.1"
                     /translation="MASISDATSDVLSVLSTVSAKHKVESLAATLSQLRRFVGSDLAE
                     VEAGLATIEHGDTPMHDSAKHLLGQKGKRLRPLCVALAARVGSGFGESARELAIAVEL
                     VHSATLLHDDVIDLGDKRRGVDTARVIYGNAGSIYAGDFLLVDALKRISRVGIPGLLD
                     RSLSVLHEILEAEALQLANRGNVRGSVSDYFRIVEGKTASLFRWALFAGGSAGGVPHD
                     QCLLLESYGRNLGVAFQVIDDVLDLAGNPEVVGKSLFADLHEGKMTYPLLLAIEREPS
                     LGEELAAACEQAALCLSPQIEKHLTAVLWEKGVVEQCVSLARMRSAEAIAALSSLPAS
                     RARAALEQVALALVYREK"
     gene            complement(17668..18216)
                     /locus_tag="PKL17_05675"
     CDS             complement(17668..18216)
                     /locus_tag="PKL17_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44250.1"
                     /translation="MMHISHSFQEAELLIADAVGEVIEHWGFRKALGRVWTVLYLAGE
                     SLSAAELAERLSMSAGAVSMTLTELQEWAVVRRVWRPGERREFFEAETDLWKMISKVV
                     AERERFWVRSVRERIEQACGVLRNLPTEQARGALLRAKVLLSFAQLAEGVIDRFLASR
                     RADFSGFGVLEKTPKTASSERR"
     gene            18430..19404
                     /locus_tag="PKL17_05680"
     CDS             18430..19404
                     /locus_tag="PKL17_05680"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNK44251.1"
                     /translation="MRTRALILSAALGLVPLSLPLFPVQNAEARPIRRPGVHALLQDL
                     AQIEAFNGERDDQRIGQQIHQQIIAVRINLLRTLSSQEPMHQAGRATMEGLYNMAALN
                     NRSFDTDQEVVWRIAGSIEHMRSRLEQLLMRHGPYQAVPVPRDYRAPVGYQPPVYRGP
                     VWDPFQGGYVPSYPGQGGVVQPVPPAPPSPPIYQQPPSPPPPVYQQQPPPPPVYQQQP
                     PPPPVYQQPAVMPVHPGQFGQLLSQMQRLSFSDEKLNYSRDLVNSGYYFTCEQIVQMM
                     KTSAFGDEQVKIGSTLYPRAVDPQNFMQMTSALTFESDRQKLRRLIGK"
     gene            complement(19405..20625)
                     /locus_tag="PKL17_05685"
     CDS             complement(19405..20625)
                     /locus_tag="PKL17_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNK44252.1"
                     /translation="MFWRAMDKKEYLAKVHTAVKQNLRYSQADVLGFPGSFLDQEIFP
                     VEQFVEEHPFLLCLRENPNHIGCHTLTESERAFSGTQALEVDLIRLCAEEIAGAPALG
                     YDGYVGSGGTECNIEAMWIFRNALYKQGISPHELVLVCSEDTHYSAHKAANLLGIDLL
                     QVPVDENSRQMEKSVLAAQITEAKRRGKRAFVCILNMGTTMFGSVDDIDSFCAVLDGL
                     GVEFRIHIDAAFGGFVYPFSTPVHRLDFRNPRVLSMTMDAHKMLQAPYGTGIFLCRKG
                     MLENVCTEAAAYVHGKDYTLCGSRSGASAVAVWMILQAYGSEGGIRFVKGLLERTDRL
                     CAGLTRLGISYFRAAGMNVVTMKAQQIPPAIAERFTLVPDSHEGTPRYVKAVVMDHVT
                     EAAIDRFLGELENS"
     gene            20824..21654
                     /locus_tag="PKL17_05690"
     CDS             20824..21654
                     /locus_tag="PKL17_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44253.1"
                     /translation="MTKRFSLRMIPLCAGLLLSGTFAGCGGEVEIAELEQQLTETKAA
                     QIVQADFDGYTNAVKLKNCTFVVAGGTATFTPSNELSQVLYGDPNGAAHKSSFAVPPI
                     TSSAATITITSMEAEMANTGITLAGTTLSVKFGFHGQLHIVATVPVFGKLPADLVIKS
                     SNLTANLGYDKATARANAQSVKANFSITTKNCGGTGWCNGIIDSILKSNLATWVETPL
                     KDSLNKALNSADVTDALNEGLTIMYNAKDPKPTKWSMAAGSLDLSGGAFRFNVSRTTP
                     "
     gene            complement(21729..23426)
                     /gene="ade"
                     /locus_tag="PKL17_05695"
     CDS             complement(21729..23426)
                     /gene="ade"
                     /locus_tag="PKL17_05695"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNK44254.1"
                     /translation="MSASSSVSAPPLDSHSQIHRGQVVDLLQQRIFPGEVFVKDGQIS
                     AIREVSQVDETDGFLMPGFVDSHIHIESSLLTPPEFARLAVAHGTVAAVCDPHEIANV
                     MGIAGVRYMQAESERTPFHFFFGVPSCVPATIFETSGATLGLAEVRELLSDPRFTHLS
                     EVMNVPGVLHGDPVLLGKLAAAQDRQIPIDGHAPGLFGEALREYVRHGISTDHECTSL
                     EEARQKLWLGQKIQIREGSAAKNFSTLWPLLKEAPRSCMLCSDDLHPDDLVIGHISRL
                     AAAAIQSGVDLFSVLHAACRTPVLHYRLPVGQLQVGDRADFVRVRDLKSLEVVETWLQ
                     GELVFHRGACLLPRLPIPICNHFSAQPIEKESLRVCARAGKLRVIVAHDRQLLTDCEI
                     LSPKVVDGAVVSDPDRDVLKLVVQSRYDKEPPQVAFVRGFGLVEGAIATSVAHDSHNV
                     IAVGADDDALLLAINRVLAERGGLCVVSKNETRCLPLPIAGLMSDADGREVAAQYVLL
                     NQLAAACGCPLAAPLMTLSFLALLVIPSLKLSDRGLFDGNKFQFVDLFVETDVPHTS"
     gene            23504..24379
                     /locus_tag="PKL17_05700"
     CDS             23504..24379
                     /locus_tag="PKL17_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNK44255.1"
                     /translation="MNRRHFLSGVGLALASGCAGPGRFVPSDSHGFVAHQINTPQGPH
                     RFFVYRPRYHSGSEKLPVILYLHGGGERGEDGVSATQVGLGPVAQASLGYFPFLVVFP
                     QCKSGGFWGMPDMAERAMQALSVAIHDHGGDPERVYVTGNSLGGYGTYLLAARYPDRF
                     AALSPICGGVKPPPLIRLPKEATLFDLQGDVYAQLAERLGRTPVWIFHGGSDPLIPYR
                     ISIQIAQALREHPGRSDPGLTQLTIWPKVGHAAEEPTYNLPQLFDWFLKQRRGKPDPS
                     AGYLAAQLEPQLSQT"
     gene            complement(24401..25060)
                     /gene="moaD"
                     /locus_tag="PKL17_05705"
     CDS             complement(24401..25060)
                     /gene="moaD"
                     /locus_tag="PKL17_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNK44256.1"
                     /translation="MKIAVRYFAHLRERLRRESDSIELADGSTVGDLLLLLGEREPLV
                     AASRRSLQVAVNMEFMPAKTQLKDGDEVALIPPVAGGSALVRLSRQPLQYDEVIAAVS
                     GPGQGGIVLFAGLVRDHNDGKQVVRLDYEAYDEMAVRKMAEIAARIESEQPGVRVAMV
                     HRVGSLQIGDLAVILAASAPHRGEAFAACRAAIEALKVEVPIWKKEFSPDGEEWLGQG
                     G"
     gene            complement(25097..26404)
                     /locus_tag="PKL17_05710"
     CDS             complement(25097..26404)
                     /locus_tag="PKL17_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNK44257.1"
                     /translation="MDQKALLAKLSQHTAHVTVIGLGYVGLPLLVEVARAGFHVTGYD
                     LIEERAASVNRGESYIKDVPSSILSELRAQGRIDATTDPRCLGRSDVVIICVPTPLNK
                     SRSPDNSYIVAAAEALLPHLHKGMLVVLESTTFPGFTREVLLPRLSETGLVVGSDFYL
                     AFSPERVDPGNARFHTKNTPKVVGGTTPECLTMIRALYGEVMDKLVPVSSTDAAEMVK
                     LLENTFRAVNIGLVNEVAIMCEKLGLDTWEVIDAAATKPFGFMPFYPGPGLGGHCIPV
                     DPLYLSWKLRTLKYEARFIDLADQINTGMPEVVVSRLQDALNDHRQAVRGSRLLVLGV
                     AYKRDIDDVRESPALDVIELLWGKGATVEYHDPYVPSLRIGSHLLQSVPLGNLGDYDA
                     VLILTDHSSIDYGAIGRSAKLVLDTRNATRNVDKTQSCPIVRL"
     gene            complement(26449..27213)
                     /locus_tag="PKL17_05715"
     CDS             complement(26449..27213)
                     /locus_tag="PKL17_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNK44258.1"
                     /translation="MRSSVRSDSSTTTSAPLADRFLAMTERDPESDESLPYRLLTPAA
                     SDAPTEKVPLVVFLHGAGERGDDNCAQLQHGVAEFLSADDRRSAYPCFYLVPQCPPGQ
                     RWVEVNWDAPQHQLPDSPSRPLRLLMRLIDRLARDPRIDSSRIYLLGLSMGGFGVWDL
                     LCRRPQLVAAAVIICGGGDEQQAAKMAHIPQWVFHGAQDAVVSVSRSRNMVHALQKAG
                     GQPRYVEYPMVAHDAWGPALREPELIPWLWRQTRTR"
     gene            27258..28310
                     /locus_tag="PKL17_05720"
     CDS             27258..28310
                     /locus_tag="PKL17_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44259.1"
                     /translation="MWTWFGVAGAAAFLLLLAGWVLRRGTPAQHAALAPADTPPHDPS
                     GSDANTTSDDDQTAAADAQTSSDADPSHSETETDSHDETDGETGDENTDRRKPILHSR
                     HWAVLAGEQEPHRFLISAFEKLLKPGSGSRATGEGRYWHLVDNSGRLQLSALVRKGEV
                     LEVLAIYPYAETTNSWPLLLERIEESPDGLQARLCGRSGGAHFAVFDTLYFKNQRHYA
                     VGETYDLQVSAIGYQLELDEPAEHSAVGSGFGSLPALSSDREAAEDEIVFHSTVEDVF
                     DCEFWGIELQVYLVTLLHGPAGPLRVEMYVHPSLTQQRFEIGDRVRGVAWLFGLWPDP
                     RATALAEALRAARLPN"
     gene            complement(28330..28866)
                     /locus_tag="PKL17_05725"
     CDS             complement(28330..28866)
                     /locus_tag="PKL17_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44260.1"
                     /translation="MDSAKQSESAPTNNPSANEPSGPIGRLSHDHIDLEAEERRHRRE
                     KRAGQPVMAIILGLTLLTAVIFYVLHLRAVAKEEAEAKQRALAAAALAEAAAKEAQKA
                     VPPPAPATAPATAPAATPTPPATTDKGAAPQPAKPAEAKPAEAKPATKAEKPARPAKK
                     ADDSKTKSLPRLPVLPEG"
     gene            29142..>32353
                     /locus_tag="PKL17_05730"
     CDS             29142..>32353
                     /locus_tag="PKL17_05730"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNK44261.1"
                     /translation="MIGSLLGLFSLLSACQSEDAVLLQIRPPSGVTINEYAVTVQDHD
                     KHVIVYQSGIQPVSAVSKGRDLFAAPLKLGLKLSTKANYLIHVRASANKLIPDGVIPT
                     SRDDEYFFAAIVNVTGTQQLDADLLDIDPQYDVDFDHFPDATKWTAAVPAAKVTYQTK
                     MNVLDCVDKDPDPTQPKLPTGLYAVDINPLAKPRCGLPFDISCGDSVPPCQDIDGDGD
                     PESTDCDDNDPNRFHGNARPRNCCTCTDKASCAKDHTKLADMSKCMPERCNSDVDYDC
                     TGKRVPCFVDDDCDGYSPNDPVVTQRDCDDTNPNIYPGAPKNCADTSKDWACDGNPSG
                     GCVDCDLDGDGYQRNDKAQGGICPTAAYTSSGKKIDCDDNDRGVFPGSTMYNGPSQIM
                     GFADLAGKEGGGSKVAAMRRLCWNTLVDGTTAQDADCDSTARNGCPTAACDVDGDGFP
                     KDNTPACNPGGLLPVDSNDADYQSFSGAPDHCGDGKPQNGTSDTPCTNDKDGDGYNAD
                     ADCDDNDPNSHPWATELCDGKDNDCDGLIDELNPDDKGARLVEKSPVTGKNVIMSCTD
                     YYRGECNNTVAATGLKTGRCVCSGIVPGKHDSNALRKACPATVDTAATAPKCFGAQQP
                     TKQTCEAVVANRKDQDCDGRLDAPDGMNLLEYGQACGVNTGTCIAGTVNGCDYTKTNP
                     FSIATGYAVVPAFDEARKYLTCTGATGPSKQLCDGKDENCNNVDDDCSDYGAANSACC
                     KNVAMCVDVAIDFAHCGNCSTACDTATANACVNKQCMCGANPACNGARGMCKNGTTCV
                     ECMADADCGDPAKPICKGQDHCVECAANADCAASANGPACDSTAEICVACTAGFGCNG
                     ATAHCKVNANPTLNKCVQCLTNSDCSGGTPACDTATNTCVPCLTGFGCSAPNSQCKLG
                     ATSAMNQCVQCLVDGNCSGSTPACDTTVNKCVTCTAGNKTQCTGSTAQCLVNAVDPTQ
                     NKCVACTANAQCTVSPRLLCDIGGQYKCASCLKDMDCGGTTPFCYQDAVDVTKNVCTA
                     CNADMDCGAGKFCFVQTNPANNFCGDCKTDADCPAMNPAC"
BASE COUNT         6970 a        10461 c         8978 g         5944 t
ORIGIN      
        1 gctttcgcca aacgaagtcg cgctgctgtg taccggcacc caaggggaat ctgtcgcggc
       61 gctcggcaag ctggtcgcag gtctgtcgcc cgattcggca ccgccgtttg gcaagctgcg
      121 actttcggct ggggactgcg tggtgctggc ggcgcgggcg attcctggca atgagcgggc
      181 gattgcgcga ctggccgacc gactggtcgc ccatcgcgtc gaggtcgtct caggcccgca
      241 ctattcggtg tccggacatg gctatcgcga cgagctcaag tggctgttac agacgtttgc
      301 gccgcgcacg cttctgcccg tccacggtgg accgcgccag cttgccgcac acgccgagat
      361 cgcgcacagc ctgggaattc ccgcgctggt ccagcacaac ggcgacatcc tcgaactgac
      421 cgatgcgggg accgtgcagg tggtgggaca gctgccaagt ccaggcctcg ccgtcgaagg
      481 aaccacggtt ggcgaggtgg gccaagatac cctgcgactg cgccggcagc ttgcccagac
      541 gggtcttgtc atcgtcgcgc aggtgtttgg caaaccgcat cggctcgcgg ttcgtgccgt
      601 cggtatttct gatccggggc cgcagcttga tgcgctgtgt caaaaagcgg cgcgtcttgg
      661 tgaagaagtc atcgcagcgc tgcgcgggac tgaaccaacg ccgccggatc tccacgagct
      721 ggagcgcgca gtcggtcgcg ccgtgcgcgg agtgtttcgc gtcgcccgtg gcatcaagcc
      781 gatggtgcag tgcgtgctct gtgaaaacag cgcaggcgac gaactaatcg aagctgccgc
      841 ggtcggtccg tcgccacagc agatggtcga caaggactaa atacaccatc gcttcaagca
      901 ccggaatcgc gcgcggcaca atgcacggat cgtgacggcc cgctttggcg gtatcgagca
      961 ccgtcgccgt cggcttacac gcagcccgca ggacaattcg ttcaccggtg ctgatgccgc
     1021 cgcgaatccc gccataacga tccggtgaat cggcggcacg atggaactcg ctgccttcgg
     1081 ctttggccgc cagcatgccg tcgcccaatt cgacactgct gaccgctccg acgccaagca
     1141 gcgccgccgc cagatccgac ttcagcttgt gaaacaccgg ctgtcccagt cctttgggca
     1201 ccccatcgca ccacagctcg accagcccgc cgtagctttt gccttcgcgc ttggccgcca
     1261 aaagaagctg ctcgacctgg gttttcaggc gaagactcgg cagccgcgcc ggaaagtcgt
     1321 cgatctgccg caccgccggt ggatcggctt gcagcgccgc cacttcatct tccgaaagtg
     1381 aaagcggccc aatctgtcgc gcaaaaccac gcaccaccag atcgggcgca agctgccgca
     1441 gcaccatctg cgccaccgcg cccgccatca cccgcgccac ggtttcacgc cccgacgatc
     1501 gccccccgcc gcgcggatcg gaatggccaa acttgtgccg ccaaacatca tcggcgtgac
     1561 caggccgagg actgcgcgca atgtcggcat agtcctgcga tcgtgcatcg aggttgcgca
     1621 ccagcatcgc aatcggcgta cccagcgtct tgcctgcgta gacaccgcta agcacttccg
     1681 cggcatcggg ttccgcgcgt gccgaagtca gactttcccc cgtcgcggtc ttgttcccag
     1741 gacgccgccg ctgaagttcg ctctgcagaa gatccggcaa aaacggaatc cccgctggac
     1801 agccatcaat gaccacgccg agcgccgcgc cgtggctttc accgaatgtc gtcaccacaa
     1861 agtgctgacc aaatgagctt gcgcccatgt ttaggagtcc tttttggaaa gagtatctgc
     1921 cgcaagatcc gcaagtccgg caattgtcaa aaactccgga aagctcttgt gaaccgctga
     1981 aagcccggca atttcgacgg gaaatcccgc ccagcgcgcc aaagctgctg ccatcaccaa
     2041 gcgatgatcg gacccggcat caaactgtcg cagcgtgctc cgatcggaat ccgcaagcgg
     2101 gcgtcccacg atctcgattc cgtcttcgcg cggcgtatgt tcacgaccaa gcacttgcaa
     2161 aagcgcgctc gtcgttgcga tgcgatccga ttccttcccg cgcaggtgcg gcgcgccaaa
     2221 cagccgtgac gttccttccg caagcgcgca caacaccgaa agcactggcg taaggtccgg
     2281 cgcgcccccc acatcgactt caatcgcgcg gagccgttcg gcgcgctgta ccaccagacc
     2341 tttcgtcgac aaatcaagcg acacacccat cttttgcagg atgtcgacaa agcagtgatc
     2401 gggctgaaga ctatgtaaag gaaagtttac aatgtgacaa gttcccgcga cagccgcgag
     2461 cgccgccacc gcaaacgccg agctgacatc ggcttcgcag ggaagtaccg ccggcacaag
     2521 acgctgccgc ggcggcacaa taagaatcat tttgtctgct tcatattgac gctcgatctg
     2581 cataccacag cgctgcgcga catcgatcga cagatccaaa tacccttccg atatgggttc
     2641 cttttcacac aaaatgtgca gcgggaatgg cagatcccag gcattcaaaa tcagcgacga
     2701 agcaaactga ctcgacagcc cagaaagcaa ggacagtcgc ccgcccggat ctgcaaaacc
     2761 cagtgaccgc gtgcgcagcg ccggggctgc gccatcgtcg cagatcgata cctgcgcgcc
     2821 aagctgccgc agcacggaaa gaaacggctc gtgcggcctt tcgataagcc ttcgcgatcc
     2881 gcacagcaca aagtctccgc cgcgccgcgc cgtgtatccc agacacagac gcagcaccag
     2941 acctgcctcg ccacagtgaa tccgcgtctg tcccgctgca aacgccgcac aggccccttg
     3001 catggccacc acatcgctac agcgcgaaag cacaggcagc gtcaccgccg gttctgtaag
     3061 ctggagcaaa aggccccgga tcagcagtga cttcgacggg ggaatcgttc ccgaaaacga
     3121 aaaaggaagg cgatcggaat tcatgattgg ggctccacat aacccatttc gatcgcggcc
     3181 tgggtcagct cggacagcgt caccggaatc acttcggact gtcccatcgc gcgcaaaaag
     3241 acaaagcgga cttcgtcctt gctcgatcgt tttttgtcct gcgccagcaa gcggacaaac
     3301 tgtgcctgtg gaatccgggg cagctcgctc cgcctatcat acagaccaaa ccgatcgcgc
     3361 agtcgcgctt ccaaatctgc tttcaccgtc gcttgtagca gtcctttttg cgtcgaaaac
     3421 gaaagtgcaa agtacagtcc ttgcgccacc gccagaccgt gtggcaggcc gtgaacggtt
     3481 tccagcacat ggcccacggt gtgtcccaag ttcagcttgt ggcgaattcc cagacgctct
     3541 tgcggatcga gttcgacaat ccgccgcttc gcagaaatcg ccgccggcag ccattcccac
     3601 agcagcgcag cgcccgtttt ttgcgaagcg agaagtccct ggacccactc accgccatcc
     3661 aaataggcga tcttggccag ctctcccatc gcttcgtggg cccgctcttc tggctgcaaa
     3721 tccagcaccc gctttgtgat ccagacatgc gtcggagcga cgatggttcc gatctgattt
     3781 tttgcccgac cgacattcag cgcatttttg ccgccatgcg cagaatcaat cgccgcaagc
     3841 caggtcgaag gaatctgctg aagcggcaca ccgcgcttaa acacactcgc aaaaaatccg
     3901 gcaaagtcgc ccactgaacc accgcccagc gccacaaccg aaagccgact ttgcggcagc
     3961 ggcgctgcga tctccagcaa atgttcgacg tgctttggga acgccgccag gtctttcaga
     4021 tcctctccgg cacagacccc atagcgctgt ggaaagccat gcagccagct gagcagcgca
     4081 ggatcggcgt gctgggccag ctgctcgatt cgctgatcat acacaagcag cggcgcatcc
     4141 cccacatcat ccagcgtggg cagtgcgtca gcacagtgaa cacttgtcgg cccattatga
     4201 agtattttaa atgactcttt ttgaacaaag agctggcggt agagcgacac caccgcggcg
     4261 gcggcttgct gcgccgacag cgcatctacc gacagccgaa catcgacatc gcgataggca
     4321 ctttcccgtc gcacaagcag atcaggaagc gtcttttttg gatccggaag cagcagcggt
     4381 cgcagcgccg gactctgcgc aagccgcgcc aacagcacat cgatcgaggc atccagacca
     4441 cacacaaaac ccagctttcg cagccgtgcc cgcacttgtg gactggtgac cagaccgccc
     4501 cctaccgaca gcacgcacgg ggcggaatcc agtgcattca caaacgcaag ctcgcgctcc
     4561 cgcagcgccg cttcaccgtc gcggaaaatc agctctgcga gcggcattcc ggcctgcgca
     4621 gacaacaccg catccgaatc gacaaacggc agcccgagca gctcggcgca gatccgacca
     4681 atggatgtct ttccgacgcc aggggggcca ctcagaatca gcatggcgcg gtatactgcc
     4741 acctatttcc ggaatctgct agtagtctgc ctgcatggat gaaaaacagt acgcgcaaag
     4801 ccaccgcgga gccacgctca cggtccaaat ccagcccgga caaagcccgc aagatgtgct
     4861 gcgtaccgtc caaaaatcca tcacggaagg actgcgctac gtagagctgc gcgatgacct
     4921 gatctgtgat gaggaactgc tgtggataag cgacaggctg ccgcgcccca caaagctgct
     4981 gagtctgcgc agagcccgtg aagaagagcg aagtcgtctg agccttatcc gtcgctgtca
     5041 gcccgccctt cttgatgtcg cgctggagct tgggcacgat gcgaaccggc tctattcgct
     5101 gatcgaaaca actggttccg tgcgcggagt tgtctcgtgt catgcgcgcg atcgcagcgt
     5161 tccttttgca caagcgctgg ccaatctttc caaacaagtt ccgtcccatt ttgcgacaaa
     5221 agctgcgctg ccgattgaca acctggccga acttttcgac ggacacaggt ggcatttgca
     5281 agatccacag cggaatctgt ttctgcccat cgcaagtgat ggcagcggtc gtttccgttt
     5341 ctatcgcttg ctgctgggtg aatcgcttct gctgaatttc ctgcgcgatc cctgcgctcc
     5401 cttgattccc gatcagccca ccctgtcgga atggcagacc cgtcatcacg tcccgatccc
     5461 aacacaggga ccgattccgt ttgccgcgat tgtgggcgat cccgtcgagc acagccgcac
     5521 cccagcacat catggatcct tttttgcgcg ctacggaatg ccggttctca aagtccggtt
     5581 gacctcttta gacctctcgt ccgatgtcct ttcgattctg ctttcactgg gtctgcgcgc
     5641 ttgtgcgatc acgtctccgc acaaaacctg gctgcgggac agcatcgatc aacacagcgg
     5701 catgtgggaa gtcagcgatg atcgcgatcc gcagtctgcc ggcaacaccc tggtcgcatg
     5761 tgacgacgga accgtattcg gaaccagtac cgatggaatc ggtctgcgcg cggcctggga
     5821 agagaccctc atcgcgcagc gtgaacgctg tggacagaat ccaggcatcg ccgtgtttgg
     5881 tggtggagga cttctgcccc tgctccgcac ggtgtttccc ggcgcactct ttttgtcggc
     5941 gcgcagtggg aagccgcggg atgcgtcacc aagcggacct gcggacaagg acatcacagt
     6001 ccttgtctgg tcggtcggcc ctagtcgctt tgtgcacaat ccaccgtcag ggctgcgacc
     6061 caagctggtg tttgatctaa actatgcgga agactctccg gcgcgcgcct atgcccagtc
     6121 gatcggtgcc cagcatgtcg atgggagtct gtttttccgc aaacaagccg acgcacagca
     6181 gctgttttgg cagcgacact tgggacattc ccaggcagca cgcgatcacg tagactttgc
     6241 aaaaaggagt ccgcaatgag cgcggttttg tattcccatc cgatgatcgt accgttccaa
     6301 gacatcgacg ctgcggggat cgtatttttc gcccgcatct ttgattactt tcacaatgca
     6361 tttgtcgagc acttgcgggc ccgtggcgtg agtctgcctg aagtcatcgc tggcggagaa
     6421 tggggagccc cgctcgccca tgctgaagct gactacaaat ttccgctgcg ctttggcgat
     6481 gaagtgcgtg tggaattggt gtcagcagac ctgggcgaca catccgtcac cttgcatttc
     6541 cggattgtgt ccggaagcga tccggtgcgc gtgcacagct tcggaaagac cgtccacgtc
     6601 accatcgatc gcaagacgtt tagaccgcgc ccggttcccc ctgccctgcg cgcagcgtac
     6661 aaggcgactg caccatcgga ccaaagcgcg tgatggcacg cagcctgtcg cgctttccgt
     6721 ttggcagttc cggaaaggaa tcgaccacca caaagcgacg cggtcgctta aacttcgcaa
     6781 gccgctcggc cagcgccgat tccatctgct tctgatcgaa gtctttttcg acgaccaagg
     6841 ccgcacagac cagcgccccc cagacttcat ccggaatccc aaacaccagc gcatcacgga
     6901 cttgcggaat cgtcaacaga gcctgctcga cttccgcagg atagacattt tcaccgccgg
     6961 ttacgatcag atcggttcgc cgcgcatgga caaaaagcgt ccccgaatca tccagctcgc
     7021 ccagatcccc tgtatccagc cagtcgcccg caagcggtgg ccgatgcaga tacccagtca
     7081 tcacactgcg gcctttcacc acaatctggc cgacctgtcc ggaaggcaga aacgatcccg
     7141 cttcgccgcg gatcgcaacc tctactccct gtagcggagt gcccgatcct cgtcgagcca
     7201 gcggcttcgg aagccacttt tgcaccgtca cctgagagca cgcttccgta agcccatacg
     7261 tacacagcac attgacccca cgatccactg cgcgcgaaag caagggaaac ggagtcgcgg
     7321 ctcccccaca gagcaccgcg cgaagacgcg acaaaatccc ccgttcgtcc gcttccagca
     7381 gcgcatgcag catggtcgga accaccgaaa gcagcgtgac ccgatgcgct tctatggcat
     7441 ccagtaccga cgctgccgaa aagcgtggca gcagcaccat gcaagctctt gcgagcacgc
     7501 agcgcgtcac aatcgaaaga ccgccgatgt gacagagcgg cagacagaca agccacctgt
     7561 cgtcttcgca aaatccgagg ttttccgcac ttccttttgc gctggcccaa aaagcagaac
     7621 gcgaaaggat ggctcctttg ggtgttcccg tggttccaga tgaatacaga atcgccagcg
     7681 tttcttcgtc ggaaatgacc gcgtgtgaag acagcccagt tgatgatgca tcgatcaggg
     7741 aatcgacatc ggaatccgca aacacccgat cgaccgctgc gtccttttgc acaaacgaaa
     7801 gctcatgatc tgtgtagcgc ggatggatcg gcacaaacgg tgtgccgcgt tcgataagcg
     7861 ccagcagaag aacaacggtc tgccagcggt tgctcgcgcg aatcgccacc acgtctttgc
     7921 gcggaatgcc acgctggtcc agtgctgcgc agaccgaaaa cacccgctcc gccagatcag
     7981 aataggtata ggcgccatca ggatggatca gcgccagccg atggggagcc tgctgtgccg
     8041 cagtccgcag acctacgtcc atggcaaacc tgcgacggga aaaccaagtc cggggccgcg
     8101 cgatggcgca atctgggccg cagattccgc atgatgagga atcgccaccg caggaaaagc
     8161 agacagcccc ggatgtgcat ccaagccgca cgcaagcggt gcagaaggca atgacagcgc
     8221 cagctcacac gcggcagcca gaccgatcgg tccgtcgagg gaatgggtga tcaccacgcc
     8281 ttttccgcac gccgccgccg caagtgcaag ctgccgcgcg cagcgaaaac caagcagcgc
     8341 aggctttagt acaaacaccg aacagtcgcg gctctgcaac agcttttcgg cttcgattgg
     8401 gtgcaaaagg gattcatccg cagcccaagg aaccgcgcag cttcccagcc ggtcaagttc
     8461 ctgtacagcg acgggctgtt cgacaaactc tggttcccat tcggcataac aggaaagctg
     8521 cgcacgcgcc ttttccagcg gccatgcgcc gtttgcatca agccgcaagc gcagcgactt
     8581 tcccagccga ctgcgaagct gcgacagaat atgaagctcg tcagatgcct ctttttttgg
     8641 atcaccgact ttgactttga tcgtccgaat tccgcgctgc cacgcagact ctgcttcggt
     8701 caggctctgc gccaccgcac tgcgcggaat cggctgcgcc agaccttgaa acagggtcgc
     8761 aatggaaaca gcatggcgac gcgccagaag atccagatac gcggtctcca gcgcaaagcg
     8821 ggccgccggc agcgcggaaa gtccgtcggc caccggccag ccttccgcat ccatcgtgat
     8881 ccgtttgtga accccatgga gcgcagacat acactgcgaa agacactctt gcgagtgacc
     8941 cacaagcggt gccgcttcac ccacgccggt ttgtccatcg gcatctacga gctgcaatac
     9001 gaccgcttca cgatccaaaa acgtgcgctg ggaattcgca aaagagccgc tgagcgagcc
     9061 ccccacaagg tgaagccgcg acgccacaat cggcgatggc aaagagagcg cggcgattcc
     9121 catcatggcc ccttactgcg caagtccgat cgcaaaaagg attccgtgaa aaagcagaag
     9181 ctgcgccgtt ttcgccagcg tctgattcag ctgtgcgccg gagtcctgcg acaccgaacg
     9241 aaccagcggc agcgccagcg gcagcgtcat cagtggcaaa agcaccgcgc gaccacactg
     9301 cccggtcatc atcagaagca ccggaaccac gtaggccagc atcacaagac ccacatattc
     9361 gcttacgcca aacgatcggc caaagcgcac caccagcgtt cgtttgcccg ccaccacatc
     9421 ggtcgctccg tcgcgcaggt tgttgaccac cagcaccgcg gtcgcaatcg ctcccaccgg
     9481 aatcgacgcc cacacagcca gcgcgggaac cgaccctgct tgcacaaaca cggtgccgca
     9541 gactgcgaca aaaccgaaaa acaggaagac aaacagatcg cccagaccgt tatagcccag
     9601 cggatatgga ccgccggtat aggcgattcc cgatgcgatc gacaaaaggc cgatgatcaa
     9661 gatcacaggg ccagcagacc aggtcagata aagccccagc accgtcgcca gtgcaaagct
     9721 tatgtacatt cccaagcgga cgttgtgcgg cgcgataaga cccgcttgcg tgacccgcag
     9781 cggacccagg cgttcggcgg tatcggcccc cttttcatag tcaaacacat cgtttgccag
     9841 gttggttccg atctggatga agatcgcgcc aaacagcgcc gccagcgcag gacaaatccg
     9901 cagcgcatgc atcgatcgcg caatcgcgca ccccaccaag acgggaatca gccccaccgt
     9961 cagtgtcgct ggacgggtgg ccaagatcca cgccttcaca gagttctttt gtacgccctc
    10021 tggaatgcgc tgtgctgcac gctgtctgct catgggaatc gctctttttc tgcgttacgg
    10081 aaagcgcttg aacttgcgga aattcggctt gcgcttttcc aaaaatgcct cttttccttc
    10141 gcgtccttct tcggtcaggt aatacagcag cgtggcgctt ccggccagct cttgcagacc
    10201 cgcttgtccg tcgcagtcgg cattgagcgc cgccttcaaa aagcgcagcg cggtcgggct
    10261 gttggcgagc atttcgcggg cccacttgag cgtttcctct tccagcttgt caaccgggac
    10321 caccgtgttg acaagcccca tcgaaagcgc ctgctgcgca tcgtactgac gacagagaaa
    10381 ccagatttcg cgcgcttttt tctgaccgac aattcgcgcc atatacgaag cgccgtaccc
    10441 accgtcaaag ctgccgactt tcggccccgt ctgcccaaag cgcgcattgt ccgccgcaat
    10501 cgtcagatca cacagcatgt gcagcacatg tccgcccccg attgcatagc ccgcaaccat
    10561 tgcaatgacg ggcttgggaa gcgtccgaat ctgccgctgc acatccagta cgttaagccg
    10621 cggaattccg tcttccccga cgtaaccacc ttgaccgcgg acccgctgat cgccgcccga
    10681 acagaacgcc tctttgcctt cgccggtcag gatcaccacg ccgacatctg catcatcacg
    10741 ggcatccgta aacgccgcag agagctcctt gaccgtgcgc ggacggaatg cgttgcggac
    10801 ttccggacgg ttgatcgtga tcttggcgat tccgtcgtcg gtcttttcat actttacgtc
    10861 ttggaaacca agatcgactg tcttccaggt tgtgtcactc atcgttgcga ttctccaaaa
    10921 ctgcgcgtga ggtgagacga aaaaacagcg agcatctgtg ctggggcttc gagaatcgga
    10981 ttgtgtcccg accccgatat cacatgatgt tcgatgaagg gatggatcga cgttagcttc
    11041 tgcgcgatcg tcgaaaactt ttcatcgact tcgcccgtaa ccagcgaaat cggcatctgt
    11101 agcgatggga catggggcca caggttcggc attcggcctg ttcccagcgt gcgaagtgcc
    11161 cacaaaatcg cgtccggtct gtgctgcaaa cgcgacaacc gctgttgcag ctgcgacgtc
    11221 tgcgaaagcc tattttgtga agcaaacagc ggcagctttt cccattctga aacaaacgat
    11281 gggagtcctt tttccaatag caaacggacc cagcggtctt cccactgaat tcgcgcttgg
    11341 cgctctgcat cgtccacaat tccggggtcg actccgatca acatcagcga cgacacacgc
    11401 tgcgggtgcc gaatcgccag cgccagcgcc aggcgggctc ccatcgaata tccgcacagg
    11461 ccaaaccgcg gcggcttgat tcgatcggcg atctgatcaa tcactgctaa aaaatccgca
    11521 tccggcagcg tccacggact gggtccatgc cctggcaaat gatcgatcag cgacggaatc
    11581 gacagcgact ctgtgggttc tgtccacagc gcaggactgc ccagaaagcc gtgcagaaac
    11641 acccacggct gcgcttcact gcgcgccttc tgggaagaag gctgcggcga tcttttccag
    11701 attcgttttc gccgcgcaag tccatcgtca cccggaacca ccgcttcaat gatcaggggt
    11761 ctttccgatt cgattccctg cgcaagcgcc gcttgatagg actgcgtgga cgcagcccgc
    11821 acaaacggaa tcccaaacgc cgcggcggcc tgcgcaaagt cgatcgtctg cggggtcaaa
    11881 aacagcttgg caaagtgcgg cgcagcagcg gagcttgaac caaccggaag ctgttcaaaa
    11941 atccgcccgc cgccattttg gacaatgacc accacaagcg gtcccgacgc ctgtgcaagc
    12001 ggagcaagtc cgcccagatc atgctgcgcg ctcagatcgc caagcagcag cgcaacggtc
    12061 ttcgatgtct ggcttttgac acccgccgcc gtggcaatca aaccatcaat gccgctcgcg
    12121 ccgcgctgat gaagcacacg cagcggcttt tcggaaggcg gacaaaacag atccagatcg
    12181 cgcaccggaa ggctgttgcc gacaagcaaa accgattcat caggaagcga cagacacagc
    12241 ccgtgggcga cggcgccttc gctcaagatt ggatcggaaa gctcggcctg taccacctcc
    12301 cagtgccgct tctctgcttg tgcaaacaga ctgtgccagc cggtatcagg acactgcgcc
    12361 ggcagcagat cacagacttt cgcaaggagc gcgaccggat cggcgttcac atgttgtgca
    12421 gtcccaatcg ggtcctgtac accgtgcggt gcaatcacaa cacgccggca gtctgcccat
    12481 ctgctggcaa agctcgcata agatgaagag accggaaaac caccaatctc gacgagaagt
    12541 tcaggttttt gatcagtcag ttcggcatcg cgcaaaaacg catcaaagcc accacagact
    12601 tccgcacgta taccaaaccg gactccgctt gtggattccg caaagatggg aaagccggtc
    12661 tgcacagaca gccgctgaag cagaacccgc agcgcttcac acgacgtgtg aacatgcgca
    12721 ggaccacaga tgatgattcc gcgccgcttg tccttgcaca gtgtcacaag cagctcgaca
    12781 gcggcctgcg aaggcaacag atcggataca attgccgtcg ctgcgggtga ttccttcagt
    12841 tcttcaagaa gcgcagcagg aatcccagcc gccgcgtctt ctgtgaacac tggttcgagc
    12901 ggcttgcgaa agcgcgcgtt cacatggacc ggccctggca gcggatacag cgtccgcgac
    12961 accgcctgcc cggccactcg caccacggca cgcagcgccg caccacttgg atcgggaagt
    13021 cccagctctg catagtgacg cacgtagtca ccaaacagct tgacctgatc caccgtctgc
    13081 gccgcaccgc agtcatagtc ttcccacggc ctgtcggcgg tcaccaaaag cagtggaatg
    13141 tgactctgtg acgcttcgat caccgcgggc agatagtggg ccggtgcggt tcctgatgtg
    13201 cagacaagaa cgctcgggcg tccgctctgc cgcgcttgtc ccagcgcaaa aaatgccgcg
    13261 acacgttcat ccaagatcac ctgcacgcgc aagtgcggat cgacggcggc agccagggca
    13321 agcggtgtcg atcgcgaacc cggactcacc accacatcgg tcacacccgc gcgccgtagc
    13381 gacgacagaa acagctcggc ccacgcggta tgcaggctct tgccgatcat attgttactc
    13441 ccagcgcgct gcgcatcgcg gaacatttcg cgttcgtttc cgcccattcc ttttgtgcga
    13501 tcgaacccgc gaccacaccg gcaccggcat acagccacgc acgcgaacca cagatcagcg
    13561 ccgatcgaat cgccacccag aatgccccat cccccgcaag atcaaagtag cccacagggg
    13621 ccgcgtaaag accgcgcgaa aacggctcct gtgttgacag aaactgcgcc gcccgatcac
    13681 gcggcagacc gcccaccgcc ggggttggat gcaaagccgc gaccagatcg agcagatgcg
    13741 tcgactgttt caagcgcgcc gcgatcggcg tacacaagtg atggacgttg cgcagcgttc
    13801 ggatcgtggt ctgacccacc tgtggatcgc tttcgcagtg gggatgcagc tcgcttgcga
    13861 tcgcagaaat gacatgcgca tgttcacgct gctctttgtc atcggacata agctcgcgca
    13921 caagctgctt atcatccagc tctgcccgtc gagcccgcgt acctgccagt gcctcggtcc
    13981 gcaaggtctg gccccgcttg tgaaacagca gctcgggcgt tgccccaacc atgtgtgaac
    14041 catcacggca gatcgcaaaa cgacagcagt ctgaatagga atcatctagt cttgccaagg
    14101 tctgggaaag cgaaaacaaa gccgaagagt cgatctgtaa ccttcgcgcc ggaacgactt
    14161 tttgcaaaac ccccgcatga atttccgcaa gcgccgccgc aaccatcgcg cagtattcgg
    14221 cctgcgatgg ctgttcggtc tgcacaaagt caccgccgca caccggagaa ggattcccgc
    14281 ttgatccctg cgccgcgtgc tcgattcggt cgagttcttc gagaagccgc tccgctgcgg
    14341 atagctcggc gggacgaatc acaagctgca catacgactc gtgttcgcgg acaaacaccg
    14401 tccagcgcgg cagcacaaag ctggcgtccg caaaagcgtg ccacggatcg tcggcagact
    14461 gtctggtcgc atccggtgca aagcgaaaac ccccaaacag ccgaggctgt ggcgcagcat
    14521 cgcaagcctc tgcacgcaga cagacaagct gtgtttcgat ctgagcggca cgcgctttca
    14581 cttgcgaaat cgccaagccg ccatcctgat cgattcgcac cgcttggccg atagaaatgc
    14641 aagcaaagtc agccacccgc gcaggcggcg catagtacga tgtcagcgtc gactgaaagg
    14701 aatcaaccag cgcacggacc ggggacgaca aagaaagcgg tgccgaaaga cacagcagcg
    14761 tgtcctccgc cattcccaca gacgcttttt ttgcccgcgc cagaagagtc cgcacgcgct
    14821 acttctcctt gcgcggcgtc gcgacataca gcgtcgccac accgaatgtc agcggctcga
    14881 ccgaaagaac agacagaccg cacgactcca taagcgccgc aaaatcactc gccgcaggaa
    14941 acgccgcgat cgactgttgc agatagcggt attccttcga tccagacaga agtccgccga
    15001 cccacggaac cacgctgtga acatgaaagc gcgcgatgcc gcttagcaat ccttcgcgcg
    15061 gctcagccag ttccagaatc gcaatccgtc cgtctttgcg ggtgacgcgc gccatttccc
    15121 gcagtgcgag cgcccgatca ggaacgttgc gaattccaaa cgccatgcag atccgtgaaa
    15181 cagagtccgc agcaaacggc agcgcctgcg catcgccttc ttgcaaggta atgcgggacg
    15241 caagctgttt ttgttcgatc ttgcgctgtc ccacggccag catgttgcgc gacggatcga
    15301 cgccaatcac atctacatcg ggcgcagtct gcgcgaccaa aatcgccaag tccgcagtgc
    15361 cggtcgccag atccagcacg cgatcaccgc ttgcaagttc gagcgctgcc actgtctttt
    15421 tccgccagcg ctgatcgatt cccagcgaga tgatccgatt gagcagatcg tagcgcgctg
    15481 cgattccatc aaacatctgg ccgctgcctg cagcagccga tgaatcctgt ttgacaggtt
    15541 gagtctcact catcgggtca ccgcttgcag cttgccaatc acgtcttcca gttcatcgcc
    15601 ccgcagcgct tgtggcccgt cgctgagcgc tcgccctgga tcatcgtggg tttctatcat
    15661 cacacccgca gccccagccg caagcgcagc ccgcgaaagc ggcaagatca gatcgcgccg
    15721 tccggttccg tgcgatggat cgacaatcac cggaagcttg tgaacattgg ccaaaagtgc
    15781 caccgcagtc agatccacca ggttgcgtgt ggcggcatca aaaccacgaa ttcctcgttc
    15841 acacagaacg actccgctcg cgccatgatg cagcagatat tccgccgcca aaagccactc
    15901 ttcaaccgtc gctgacatcg cccgcttgag cagcaccggc ttgccggctt ttccgacttt
    15961 ttttagcagc gcaaagtttt gcatgttgcg cgaaccaatc tgaatcagat cggcttgctc
    16021 tgcgaccaga ctgacatcct gttcggacaa cacttcggtg accacgcgaa gtccgttttt
    16081 cgtcgcagca tcgcgcatcc aaccgagcgc cggatcccca tgtccctgaa aattgtacgg
    16141 cgagctgcgc ggcttgaacg cgccaccgcg cagaaacgtc acgcccagcg gtgccagccg
    16201 cgccgcgatc gcatcgatct gttcttttga ttcgacgctg cacggaccac acatcagcgt
    16261 cggcaagcga ccaccgattg catgacctgc gacctcgacc gttggtccct gtgcatcaat
    16321 cctgggatgt tcgggaactg gcatcgacac cgcaaacaca ccgcggatct tcgccacaag
    16381 ctgtggatcg accaccgtgg aatgcggcgc caccacaaac tgaattccgc ccgaacccag
    16441 gccctgttca acggacgcaa tccaaagccc aagcgctgta agctcacgct tcaccgcttg
    16501 cggatcagca tctttcagaa gtgaaacaat catctgttgc ctctactttt ctcggtacac
    16561 cagagccaag gcaacctgtt ccagcgccgc ccttgcacgc gaagccggaa gcgaactgag
    16621 cgcggcaatc gcctctgcag aacgcatccg cgcgagcgaa acgcactgct cgaccacccc
    16681 tttttcccac agcaccgccg tcaggtgttt ttcgatctgg ggggaaagac acagcgccgc
    16741 ttgctcacac gctgctgcaa gctcttcacc caaagacggt tcccgctcga tcgccagcaa
    16801 gagcggatac gtcatcttcc cttcgtgcag atccgcgaac agagacttcc caaccacttc
    16861 gggattccct gccagatcga gcacgtcatc gatcacctga aaagcaaccc ccagattccg
    16921 tccgtagctt tccagaagca gacactgatc gtggggaaca ccaccggcag aaccgcccgc
    16981 aaacagcgcc cagcggaaca gactcgcggt ttttccttcg acgatgcgga agtaatccga
    17041 aaccgatcct cgcacattgc cgcggtttgc aagctgcagc gcttcggctt ccagaatttc
    17101 gtgcaggacg gacagactcc gatcgagcag tccgggaatt ccgacccgcg agatccgttt
    17161 cagcgcgtcg accagcaaaa aatcccccgc atagatgctg ccggcattgc catagatcac
    17221 ccgcgctgtg tccacgcccc gccgcttgtc cccaaggtca atcacatcat catgaagcag
    17281 ggttgcggaa tggaccagct cgacggcaat tgccagttcc cgcgccgatt caccaaaccc
    17341 gcttccgacc cgcgccgcca gcgcgacaca gagcggacgg agtcgctttc ccttctgccc
    17401 aagcaagtgc ttggcgctgt catgcatcgg cgtatcgccg tgttcaatcg tcgccagtcc
    17461 ggcttcgact tccgctagat ccgaccccac aaaacgccgc agttgcgaaa gcgtcgcggc
    17521 cagcgattcg actttgtgtt tggcagacac cgtcgataga accgaaagga catcgcttgt
    17581 cgcatccgag atcgaagcca acgatgattt gggaacttgt gattctgtgt ccacgttttt
    17641 tcttccgtat tccgatgact ctgcgcgcta gcgacgttcc gaactggctg ttttcggtgt
    17701 cttttccagc accccaaaac cgctgaagtc ggcacgtcgt gacgccaaaa accgatcgat
    17761 cacaccctcg gcaagctgcg caaaagacag cagcactttg gcccgcaaaa gcgccccgcg
    17821 tgcttgttcg gtcggcaagt tccgcaaaac gccgcaggct tgctcgatcc gctcgcgcac
    17881 actgcgaacc cagaaacgct cacgctcggc gacgaccttt gaaatcatct tccacaggtc
    17941 ggtctctgct tcgaaaaact cgcgccgctc acccggtcgc cagaccctgc gcaccaccgc
    18001 ccattcctga agttccgtca gcgtcatgct caccgccccc gccgacatcg acagccgctc
    18061 ggccagctct gccgcggaca gcgactcgcc cgcaagatac agcaccgtcc acacccgtcc
    18121 gagcgctttg cgaaaacccc agtgctcgat gacttcaccc actgcgtcag caatgagcag
    18181 ctcggcttcc tgaaaagagt ggcttatgtg catcatggct tgaactttca gaaagatttg
    18241 aaactactgt aacctcaaaa tgctcactgt caagagaaac gcgggcacgc cgcctgcgcg
    18301 aaagaggtcg gaccgtactg ccagccggcg acggacgtgc tgcatccgat agactgaaga
    18361 aacgtcgccg cgctatcgtc ccatcctaag gtacgtaagc gacccaaacc ctttttcgag
    18421 gtatcttcca tgcgaacccg tgccctgatt ctgtcggcgg ctcttggcct tgtgccgctg
    18481 tccctgccgc tttttcccgt ccagaacgcc gaagcgcgac cgattcggcg tcccggcgtt
    18541 cacgcactgc tccaagactt ggcgcagatt gaagccttca acggcgagcg tgacgatcag
    18601 cggatcggcc agcagatcca ccagcagatc attgcggtgc ggatcaatct gctgcgcacg
    18661 ctgagcagcc aggaaccgat gcaccaggcc ggtcgcgcga cgatggaagg tctttacaac
    18721 atggccgcgc tcaacaaccg atcgtttgat accgatcagg aagtggtgtg gcgcatcgcg
    18781 ggcagcatcg aacacatgcg ctcgcggctg gaacagctgt tgatgcggca cggtccgtac
    18841 caggcggttc cggtgccgcg tgactaccgc gcgccagttg gatatcagcc gccggtctat
    18901 cgcgggccgg tctgggaccc gttccaaggc ggctatgttc cgtcctatcc gggccaaggc
    18961 ggcgtcgtgc agcctgtgcc acccgcgcca ccttcgccgc cgatttatca gcagccgcct
    19021 tcgccgccgc cgccggttta tcagcagcag cctcccccgc cgccggttta tcagcagcag
    19081 cctcccccgc cgccggttta tcagcagccc gcggtgatgc cggtccatcc ggggcagttt
    19141 ggccagctcc tttcgcagat gcagcgtctg tcgtttagcg atgaaaagct caactactct
    19201 cgcgatctgg tgaactctgg ttactacttc acctgcgaac agatcgtgca gatgatgaag
    19261 acgtcggcgt tcggtgatga gcaggtcaag attggatcga cgctgtatcc gcgcgcggtc
    19321 gatccgcaga acttcatgca gatgacatct gcgctgacgt ttgagtccga ccgccagaag
    19381 ctgcgccgcc tgattgggaa gtaatcaaga gttctccagc tcccccagga agcggtcgat
    19441 cgccgcttct gtcacatgat ccatcaccac cgcttttacg taccgcggtg tcccttcatg
    19501 agaatccgga accagcgtga accgctccgc aatcgcgggc ggaatctgct gcgctttcat
    19561 tgtcaccaca ttcattcccg cggcgcggaa ataggaaatg ccaagccgcg taagcccagc
    19621 gcagagccga tcggtgcgtt cgaggagtcc tttgacaaag cggattccgc cttccgatcc
    19681 ataggcctgc aagatcatcc agactgccac ggcggaagcc ccagaacgac tgccgcagag
    19741 cgtatagtct ttgccatgaa cataggcagc agcttcggta cagacgtttt ctagcattcc
    19801 ttttcggcac aggaaaatcc cggtcccata cggcgcttgc agcatcttgt gcgcatccat
    19861 cgtcatcgac aacacgcgcg gattgcgaaa gtccagtcga tggacagggg tggaaaacgg
    19921 atagacaaaa cccccgaacg cagcatcgat gtgaatccga aattcgactc ccaaaccgtc
    19981 gagcaccgca caaaaggaat cgatgtcatc gaccgatccg aacatcgtgg tgcccatgtt
    20041 taggatgcag acaaatgccc gcttgccccg ccgtttcgct tcggtgatct gcgcggcaag
    20101 gacggacttt tccatctgcc gactgttttc atcgacagga acttgcaaaa gatcaatgcc
    20161 gagcaggttg gcggctttgt gcgccgaata gtgggtgtct tccgaacaga ccagcaccag
    20221 ctcgtgcggg gaaattccct gtttgtagag cgcgttgcgg aaaatccaca tcgcttcgat
    20281 gttgcactct gttccgccgc ttccgacgta accgtcgtat cccagcgcag gcgcgcccgc
    20341 gatctcttca gcacagagcc gaatcagatc gacttccagc gcttgtgtcc cggaaaacgc
    20401 ccgctctgat tccgtcagag tgtgacagcc gatgtggttg ggattttcgc gcagacacag
    20461 caaaaaagga tgctcttcga cgaattgctc gacgggaaaa atctcttgat cgagaaacga
    20521 ccccggaaaa ccgagcacgt cggcctgcga gtagcgcaga ttctgcttca ccgccgtgtg
    20581 cacttttgcc aggtattcct ttttgtccat cgctcgccag aacatatgaa gactcctttg
    20641 cgcgggtacg ccggattgtg aatcgattgc cgcaccgcgt caccactgca gcctttgcgt
    20701 cagaaagtgc agcatagaat ttgtagaaaa agcaattgac gtagtcttgt aagtttcggt
    20761 acgaactcct cttcctgtcc gtttttcttg ctaggcccaa acgcaaaccg aaggagggtt
    20821 ccgatgacga aacggttttc cctgcggatg atcccgctgt gcgctggtct tcttctatca
    20881 ggtacgttcg caggctgcgg cggtgaagtc gaaattgccg agcttgagca gcagcttacc
    20941 gagaccaaag cggcgcagat cgtacaagcc gattttgatg gctacacgaa cgcggtgaag
    21001 ctgaaaaact gcacattcgt cgtcgcaggt ggaaccgcga catttacgcc ctccaatgag
    21061 ctttcgcagg tgctgtacgg cgatcccaat ggcgccgcgc acaagtcatc gtttgcagtt
    21121 ccgccgatca cgtcgtcggc tgcaacgatc accatcacca gcatggaagc ggagatggcg
    21181 aacaccggaa tcacgctggc tggaaccacg ctgagcgtga agtttggttt ccatggccag
    21241 cttcacatcg tcgcgacggt gccggtgttt ggcaagctgc ctgctgacct tgtgatcaag
    21301 tcgtcgaacc tgaccgcaaa ccttggttat gacaaagcga ccgcgcgcgc caacgcccaa
    21361 tcggtgaagg ccaacttcag catcaccacc aagaactgcg gcggcaccgg ctggtgtaac
    21421 ggaatcatcg actcgatcct gaagtcgaac ctggcgacct gggtggaaac cccgctcaag
    21481 gactcgctga acaaggcgct caacagtgcg gacgtgaccg acgcgctgaa cgaaggtctg
    21541 acgatcatgt acaacgccaa agatccgaag ccgaccaagt ggtcgatggc ggctggcagt
    21601 ctggatcttt cgggcggcgc gttccgcttc aacgtctcgc gaaccacacc gtaaccgcag
    21661 catcccttcc gcagcactcc gcttttgcca gacagactcc gcgatcacga tccggagtcg
    21721 tgtctgtttc atgatgtgtg cggcacatcc gtttccacaa acagatcgac aaactggaac
    21781 ttgtttccgt caaataggcc gcggtccgaa agcttgagcg acggaatcac cagcagcgcc
    21841 aaaaacgaca gggtcatgag cggcgccgcc agcggacagc cacacgcagc cgcaagctga
    21901 ttgagcaaga cgtactgcgc cgcgacttca cgtccatccg catcgctcat aagtcccgcg
    21961 atcggcagcg gcagacatcg cgtttcgttt tttgaaacca cacacagacc gccacgttca
    22021 gcgagcacac ggtttatcgc caaaagcagt gcgtcatcat ctgcaccgac ggcgatgacg
    22081 ttgtgactgt catgcgcaac gctcgttgca atcgcgcctt cgacaagtcc gaagccacgc
    22141 acaaacgcca cctgcggcgg ctctttgtcg taacgactct ggaccaccag cttgagaaca
    22201 tcgcggtccg gatcagagac caccgcacca tcgacaactt tgggcgaaag gatctcgcag
    22261 tcggtcagaa gctgtctgtc gtgcgccacg atcacgcgca gcttgccggc ccgcgcgcac
    22321 acccgcagcg attccttctc gatgggttgc gccgaaaagt ggttgcaaat cgggatcgga
    22381 agccgtggca aaagacacgc accgcgatgg aacaccagct ctccttgcag ccaggtttcc
    22441 acgacttcca gcgacttgag atcacgcaca cggacaaagt ccgcgcggtc cccaacctga
    22501 agctgaccga ccggaagtcg atagtgcagc accggcgttc ggcaagcggc gtgcagaacc
    22561 gaaaacagat cgacccctga ctggatcgca gccgctgcca gacgacttat gtgacctatc
    22621 acaaggtcat cgggatgcag atcatcgcta cacaacatac agctacgcgg cgcttctttt
    22681 agcagcggcc acagcgtcga aaagttcttc gcagcagagc cctcgcggat ctgaattttc
    22741 tgtcccagcc acagcttctg tcgggcttct tccaagctgg tgcattcatg atcggtactg
    22801 attccgtgcc gcacatattc gcgcagcgct tcgccaaaca gaccgggagc atgtccgtca
    22861 ataggaatct gtcgatcttg tgccgcggca agctttccca acagcaccgg atcaccatgc
    22921 agcacacccg gaacattcat cacttcggac agatgggtaa agcgcggatc ggacagaagc
    22981 tcgcgcactt cggcaagacc aagcgtcgcg ccagacgttt caaaaatcgt cgccggaaca
    23041 caggacggaa cgccgaaaaa gaagtgaaac ggagtccgct cggactctgc ttgcatgtac
    23101 cgcacgcccg cgattcccat cacattggca atctcatgtg gatcgcagac cgcagcgacg
    23161 gtgccatgag ccacggccag tcgcgcaaac tccggcggcg tcaaaagcga gctttcgatg
    23221 tgaatgtggg aatcgacaaa acccggcata aggaatccgt ccgtttcgtc cacttgtgac
    23281 acttcgcgga tcgcagaaat ctggccgtct ttcacgaaga cttcgccagg aaagatccgc
    23341 tgctgcaaaa gatcgacgac ctggcctctg tgaatctgtg aatgggaatc gagcggcggt
    23401 gcggaaaccg aagaagaagc agacatgccg ggactataaa cgacgacctg cttgccttgt
    23461 gtccgccgcg gcatccaaac tgtggataca ttcgagaggc cccatgaacc gtcgtcattt
    23521 tttgtccggt gtgggtttgg ctttggcgag cggctgcgcg ggtccgggtc gttttgtccc
    23581 atcggattcg catggctttg tcgcgcacca gatcaacacg ccgcagggac cgcatcgctt
    23641 ctttgtctat cggccccggt atcacagtgg atcggaaaaa ctgccggtga tcttgtatct
    23701 gcacggcggt ggcgagcgcg gtgaagacgg cgtgtcagcg acgcaagtgg gtctgggccc
    23761 ggtcgcgcaa gcatcgcttg gctactttcc gtttctggtg gtgtttccgc agtgcaaaag
    23821 cggcggcttc tggggcatgc ccgacatggc ggaacgcgcg atgcaagcgc tgtcggtggc
    23881 gattcacgat catggcggcg atccggaacg ggtctatgtg acaggaaact cgctcggggg
    23941 atatggaacg tacctgcttg cggcccgcta tcccgatcgc tttgccgcac tttcgccgat
    24001 ctgtggtggg gtcaagccgc cgccgctcat ccgtctgccc aaagaagcga cgctgtttga
    24061 tctgcaaggc gatgtctatg cgcagctcgc cgagcggctc ggacggacac ccgtgtggat
    24121 tttccacggc ggttccgatc cgctgattcc gtatcgcatc tcgatccaga ttgcccaggc
    24181 gctgcgtgaa caccccggtc gttccgatcc tgggcttacg cagcttacga tttggcccaa
    24241 agtcggacac gctgccgagg aaccgaccta caacctgccg cagctttttg attggttttt
    24301 aaaacagcgg cggggcaagc ccgatccaag cgccggatat ctggccgcgc agctagaacc
    24361 ccagctttcc cagacataaa caggtggcga aacggtcggc ttaaccgccc tgccccagcc
    24421 actcttcccc gtcgggagaa aactcttttt tccagatcgg cacttcgact ttcagcgctt
    24481 caatcgccgc ccgacacgcc gcaaacgctt cgccgcgatg gggtgccgat gccgccaaga
    24541 tcaccgccaa atcgccaatc tggagcgagc cgacgcggtg caccatcgcg acccgcacac
    24601 cgggctgctc ggattcgatc cgcgccgcga tctcggccat cttgcgcacc gccatctcgt
    24661 catacgcttc gtaatcgagc cgcaccacct gtttgccgtc gttgtgatcg cgcacaagac
    24721 cggcaaacag cacgatcccg ccctgtcccg gcccgctgac cgccgcgatc acttcgtcgt
    24781 attgcaaggg ctgccgcgaa agccgaacca gcgcagagcc cccggcgact ggcggaatca
    24841 gcgcgacttc atcgccgtct ttgagctggg ttttggccgg cataaattcc atgttcaccg
    24901 cgacctgcaa gctgcgccga ctcgccgcga ccaaaggctc gcgttcaccc agcagcaaaa
    24961 gcagatcccc gactgttgat ccgtcggcaa gttcgatcga atccgactct ctgcgcaaac
    25021 gctcgcgcag atgggcaaag tagcgcaccg cgatcttcat aggggtttcc ttcacagaag
    25081 aggatcggcg cggacgctac agccgcacga tcggacagga ctgcgttttg tcgacgttgc
    25141 gcgtcgcgtt gcgggtatca agcaccagct tggcgctgcg accgattgcg ccatagtcaa
    25201 tcgagctgtg gtcggtcagg atcagcaccg catcgtagtc accgaggttg ccaagcggca
    25261 ccgactgaag cagatggctg ccaatccgca agctcggaac atatggatcg tgatactcga
    25321 cggtggcgcc tttgccccac aaaagctcaa tcacatcgag cgctggcgat tcgcgcacgt
    25381 cgtcgatgtc gcgcttgtac gccacgccca gcaccaaaag ccgcgatcca cgcaccgctt
    25441 gccgatggtc attgagagca tcttgcagcc gcgacaccac cacttccggc atcccggtat
    25501 tgatctgatc ggccaggtcg ataaagcgcg cttcgtattt gagcgtccgc agtttccacg
    25561 acagatacag cggatcgacg gggatgcagt gaccgcccag acccggcccc ggataaaacg
    25621 gcataaaacc aaacggcttg gtcgccgccg cgtcgatcac ttcccaggta tcgagcccca
    25681 gcttttcgca catgatcgcg acctcgttga cgaggccgat gttgacggcg cgaaaagtgt
    25741 tttccaaaag cttcaccatt tccgccgcat ccgtcgacga aaccggcacc agcttgtcca
    25801 tgacttcgcc gtacagggcg cgaatcatcg tcagacattc gggcgtagtg ccaccgacga
    25861 ccttgggggt gtttttggtg tgaaaacggg cattgccggg atcgacgcgc tcgggagaaa
    25921 acgccagata gaagtcgcta ccgacgacaa gacctgtttc ggaaagccgc ggcagcagca
    25981 cctcgcgggt gaagccgggg aacgtcgtcg attccagcac caccagcatc cctttgtgca
    26041 ggtgcggcaa aagcgcttcc gccgcagcga cgatgtagct attgtccggc gaccgcgatt
    26101 tgttaagcgg cgtcggcacg cagatgatca cgacatccga tctgccaagg cagcgcgggt
    26161 cggtggtcgc gtcgatgcgt ccctgcgcgc gcagctcgga cagaatgctg ctcggaacgt
    26221 ctttgatgta gctttcgcca cgattgaccg aggctgcgcg ctcctcgata agatcgtagc
    26281 cagtgacgtg gaacccggcg cgcgcgactt cgacgagaag cggcagaccg acatagccca
    26341 ggccgatcac agtgacgtgc gcggtgtgct gcgaaagctt tgcgaggagc gctttttgat
    26401 ccattgcgtt gcccgcttct atcgtacgca aagccgcgat tgcaaggttt accgcgtgcg
    26461 cgtttgccgc cacagccacg ggataagctc cggctcgcgc agcgccggtc cccatgcatc
    26521 atgcgccacc atcggatatt cgacatagcg cggctgtccg ccggcttttt gcagcgcgtg
    26581 taccatgttg cgcgagcgag aaaccgaaac caccgcgtct tgcgccccgt gaaacaccca
    26641 ctgcggaatg tgggccatct tggcggcctg ctgttcgtcg ccgccaccac agatgatcac
    26701 cgccgcggcg acaagctgcg gacgccggca cagcagatcc caaacgccaa agccgcccat
    26761 cgacagccca agcaaataga tgcgcgacga atcaatccgc ggatcgcgcg cgagccggtc
    26821 gataagccgc ataagcagcc gcagcggtcg cgaaggtgaa tccggcagct gatgctgcgg
    26881 cgcgtcccag ttcacttcga cccagcgctg accgggcgga cactgcggga caagataaaa
    26941 acacggatag gcagaacgtc tgtcgtctgc ggacaaaaac tcagcgacac catgttggag
    27001 ctgcgcgcag ttgtcgtcgc cacgttcgcc cgcgccgtgc aaaaaaacca ccagcggcac
    27061 tttttctgtc ggcgcatccg acgcagccgg ggtcagcaga cgatatggca gcgactcgtc
    27121 gctttcggga tcacgctcgg tcatggccaa aaaacgatcg gcaagcgggg cagaagtggt
    27181 cgtcgatgaa tcgctgcgca cagaagatcg catccgaaaa gtgtagtcca cctgccacct
    27241 ggctatactg ccgcgccatg tggacttggt tcggggtggc cggtgccgcc gcgtttttac
    27301 tcctgctcgc aggctgggtt ttgcgacgcg gaacccctgc acaacatgcg gcactcgcgc
    27361 ctgcggacac gccgccacac gacccgagcg gcagcgacgc aaacaccaca agcgatgatg
    27421 accaaaccgc agcggcggac gcgcagacct ccagcgatgc cgatccgtcg cacagcgaaa
    27481 ccgaaaccga cagtcacgat gaaacggacg gcgaaacggg cgacgaaaac accgatcgcc
    27541 gcaagccgat cctgcacagc cgacactggg cggttctcgc gggggaacaa gagccgcatc
    27601 gcttcctgat ttcagcgttt gaaaagctgc tcaagcccgg cagtggatcg cgcgcgaccg
    27661 gcgaaggtcg ttactggcat ctcgtcgaca acagcggacg gctacagcta tcggcgctgg
    27721 ttcgcaaagg cgaagtgttg gaagtgctgg cgatctatcc gtatgccgaa accaccaaca
    27781 gctggccgct cttgctcgaa cgaatcgagg aaagtcccga cggactgcaa gcccgcttgt
    27841 gtggacgttc cggcggtgcc cacttcgcgg tgtttgacac gctgtacttc aaaaaccagc
    27901 gccactatgc ggtcggcgaa acctacgatc tgcaagtcag cgcgattggc tatcagctag
    27961 agctcgatga accggcggaa cacagcgcgg tggggagcgg ctttggatcg ctccctgccc
    28021 tgagttccga tcgcgaggct gccgaagacg agatcgtctt tcacagcacc gtcgaagatg
    28081 tctttgattg cgagttctgg ggcatcgaac ttcaggtcta tctggtgacg cttttgcacg
    28141 gcccagccgg tccgctgcgc gtcgagatgt acgtgcatcc atcgctgacc caacagcgct
    28201 tcgaaatcgg cgatcgggtc cgcggggttg cgtggctgtt cggactctgg ccggatccac
    28261 gcgccaccgc gctcgcggaa gcactgcgcg ccgcccgact gcccaactag ttttccgtcg
    28321 gagcgaggct tacccttcgg gtagaaccgg caagcgcggc aggctcttgg tcttgctgtc
    28381 gtcggctttt ttcgccggtc gcgccggttt ttctgctttg gtggcaggtt tggcttcggc
    28441 aggtttggct tcggcaggct tggccggttg cggtgccgcg cctttgtccg tggtcgcagg
    28501 cggggtcggt gtcgcagcag gcgcagtcgc tggtgccgtc gctggcgcag gcggcgggac
    28561 cgctttttga gcttccttgg cagcggcttc ggccaaagcg gcagcggcca acgcgcgctg
    28621 tttggcttcg gcttcctctt tggcgaccgc ccgcaggtgc agcacataaa agatcaccgc
    28681 ggtaagcagc gtcagcccca agatgatcgc catcaccggc tgacctgcgc gtttttctcg
    28741 ccggtggcgt cgctcctcgg cttccaggtc aatgtgatcg tgcgaaagac gaccgatcgg
    28801 cccgctcggt tcgtttgcgg acggattgtt cgtcggggcg gattctgact gtttggcgct
    28861 gtccatcgtg cccggtcagg atatcagccg ggccggcgat ttcgcaaacc cccacgtcgg
    28921 cgcaccggga aaaagtaccg cagagacgga cgcctggcag gcagacccgg ttctggctgt
    28981 actcgcgacg gcaaagcgag ctaacatggt ttgcaagtag cttgggggag cttatgaccg
    29041 cttaccgact cactggccac gctgtatcca catcgacgtg ccttgatcgg ccttaccttc
    29101 gaccttcatc gcgattccgt cagtggctcc gaaaaaattc cgtgattggc agtctgcttg
    29161 gcctgttttc gctgctcagt gcctgtcaga gcgaagacgc tgtgcttctg caaatccggc
    29221 caccatcggg cgtcaccata aacgaatacg cggtgacggt gcaggaccat gacaagcacg
    29281 tcatcgtcta tcagtccggc attcagcccg tgtcagccgt cagcaaaggc cgcgacctgt
    29341 tcgccgctcc gctcaagctg ggactgaagc tatcgacgaa agcgaactac cttatccatg
    29401 tgcgggcgtc ggcaaacaag ctgattcccg atggcgtcat tccgacctct cgcgatgacg
    29461 agtatttctt tgccgccatc gtcaacgtga ccggcaccca gcagctggat gccgatctgc
    29521 ttgatatcga tccgcagtac gacgtcgact tcgatcactt tcccgacgcc accaagtgga
    29581 ccgcagcagt cccagcggcc aaagtcacgt atcaaaccaa gatgaacgtg ctcgactgcg
    29641 tcgacaaaga tcccgatccg acacagccga aactgcccac gggtctttac gcggtcgaca
    29701 taaacccact ggccaagccg cgctgcggtc tgccatttga catttcgtgc ggtgacagcg
    29761 tcccgccttg ccaagatatc gacggcgacg gcgatcccga aagcaccgac tgcgacgaca
    29821 acgatccaaa tcgtttccac ggcaacgcgc gtccgcgcaa ctgctgcacc tgtactgaca
    29881 aagcaagctg cgccaaagac cacaccaagc tggccgacat gtccaagtgc atgccggagc
    29941 gctgcaacag cgacgtcgac tacgactgca ccggcaagcg cgttccgtgt tttgtcgatg
    30001 acgactgcga cggctattcg ccaaacgatc cggtcgtcac ccagcgcgac tgcgacgaca
    30061 caaaccccaa catctatccg ggtgcgccga aaaactgcgc cgacaccagc aaagactggg
    30121 cgtgcgacgg caatccgtcg ggtggctgcg tcgactgcga tctcgacggt gacggctacc
    30181 agcgcaacga caaagcacaa ggcggcatct gcccaaccgc ggcctacacg tcatccggca
    30241 agaagatcga ctgcgacgac aacgatcgcg gcgtgttccc cggatcgacg atgtacaacg
    30301 gtccgagcca gatcatgggc ttcgccgatc tcgcgggcaa agaaggcggc ggatcgaaag
    30361 tcgcagccat gcggcggctg tgctggaaca ccctggtcga cggcaccacg gcgcaagatg
    30421 ccgactgtga cagcaccgct cgcaatggct gtccgaccgc agcgtgcgac gttgacggcg
    30481 acggcttccc caaagacaac accccagcgt gcaaccccgg cgggcttttg ccggtcgaca
    30541 gcaacgacgc cgactaccag agcttcagcg gcgcgcccga ccactgtggc gacggcaaac
    30601 cgcaaaacgg cacgtccgat accccgtgca caaacgacaa agacggcgac ggttacaacg
    30661 cggacgccga ctgcgatgac aacgatccca actcgcaccc gtgggcgacc gagctgtgtg
    30721 atggcaaaga caacgactgc gacggtctta tcgacgagct gaaccccgat gacaagggcg
    30781 cgcggctggt cgaaaaaagc ccggtcacag gcaaaaacgt catcatgtcg tgcaccgact
    30841 actaccgcgg cgagtgcaac aacaccgttg ccgccaccgg actcaagacg ggtcgctgcg
    30901 tgtgcagtgg cattgtgccc ggcaagcacg actcaaatgc gctgcgcaaa gcatgcccag
    30961 cgacggtgga taccgcggcg accgctccca agtgtttcgg cgcgcagcag ccgaccaaac
    31021 agacctgcga agccgtggtt gcgaaccgca aagaccaaga ctgcgacgga cggctcgatg
    31081 cgccggacgg catgaacctt ctggaatacg gccaggcctg cggcgtaaac accggcactt
    31141 gcatagccgg caccgtcaat ggctgcgact acaccaagac caacccgttt tcgatcgcca
    31201 ccggctacgc cgtcgtgccg gcctttgacg aagcgcgcaa gtatctgacc tgcacgggtg
    31261 cgaccggccc aagcaagcag ctttgcgacg gcaaagacga aaactgcaac aatgtcgacg
    31321 acgactgctc ggactatggc gccgccaact cggcgtgctg caaaaacgtc gcgatgtgcg
    31381 tggacgtggc gatcgacttc gcccactgcg gcaactgctc gacggcgtgt gacaccgcaa
    31441 cggccaacgc ctgcgtcaac aaacagtgca tgtgcggtgc aaacccagcg tgcaacggtg
    31501 cccgcggcat gtgcaaaaac ggcacgacct gcgtcgagtg catggccgac gccgactgcg
    31561 gcgatccggc caaaccgatc tgcaaaggcc aggatcactg cgtcgagtgc gcagccaacg
    31621 ccgactgcgc cgccagcgcc aacggcccgg cctgcgacag caccgcggaa atctgcgtcg
    31681 cctgcaccgc cggctttggc tgcaacggcg cgacggccca ctgcaaagtg aacgccaatc
    31741 cgacgctgaa caagtgcgtc cagtgcttga ccaactcgga ctgttcggga ggaacgccgg
    31801 cctgcgacac cgcgaccaac acctgcgtgc cgtgccttac tggctttggc tgcagcgccc
    31861 cgaacagcca atgcaagctc ggcgccacgt cggccatgaa ccagtgcgtc cagtgcttgg
    31921 tcgatggcaa ctgctcgggc agcacgccgg cttgcgacac caccgtcaac aagtgcgtaa
    31981 cctgcaccgc gggcaacaag acccagtgca ccggatcgac ggcccagtgt ctcgtcaacg
    32041 cggtcgatcc gacccagaac aagtgcgtcg cttgcaccgc caacgcccag tgcacggtgt
    32101 cgccacgact tctgtgcgac atcggcggcc agtacaagtg cgcttcgtgc ctaaaagaca
    32161 tggactgcgg cgggaccaca ccgttctgtt accaggacgc cgtcgacgta accaaaaatg
    32221 tctgcaccgc gtgcaacgcc gacatggact gcggcgccgg caagttctgc tttgtgcaga
    32281 caaacccggc aaacaacttc tgcggtgact gcaagaccga cgcagactgc ccggcgatga
    32341 atccggcctg cga
//
LOCUS       DAORGN010000032        32240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_515_length_32240_cov_6.178189, whole genome shotgun sequence.
ACCESSION   DAORGN010000032 DAORGN010000000
VERSION     DAORGN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 32240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32240
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_515_length_32240_cov_6.178189"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKL17_05735"
     CDS             <1..901
                     /locus_tag="PKL17_05735"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNK44262.1"
                     /translation="DPQCNLTFGMLGKRMFALWDRPTEACAHAAACLEPVRQGRGLLR
                     PPQLAEVADRLWLLPGHLDLSRFEQTLAEQWPRVMAADNERALDVVLSLSALAHRAAE
                     SVAADLVLLDVGPSLGAINHSAMLACDNVVVPMAWDWFSLQALLNIGPSIKEWRDDWQ
                     TVRERFLRSHPYAAFATHRFQPIGYVLQQRINQPDMDSLGYEAIGLNVQRVFRIHVLQ
                     EGRLQDGAATDPDEHRLAMVDHYAALAPLSQLFCKPMFDLTRADGAVGGQVRMVRECR
                     RTIEALARKICRRMDIPLPLPPP"
     gene            complement(1202..2143)
                     /locus_tag="PKL17_05740"
     CDS             complement(1202..2143)
                     /locus_tag="PKL17_05740"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNK44263.1"
                     /translation="MSSQASVVARRVFYLSPHPDDVALSCAGSLLADVAAGADVTLLS
                     VFVSGESAATRRAEDEQAARALGCKYLSLDLFDAPDRPEIHGSLGLFMPYGPPHLGIT
                     SEVVARLRWHVSAPAELVAPLAVGGHIDHRIVHEAARALAFELGLQLAYFEDLPYSLS
                     RFSVARRLAALETSVPRLAGTDRGTVAMERAAYRDYLCGLPLFRRYPPGLRFLLGHLV
                     ARTAVRADGQAHRPGPRPDLTAQLQTVTANSPARLAAIAAYASQWPLFAVSAEELLAR
                     VVDYGRTLGPALPDDKTYERLWHDRAFPPRMDHSESG"
     gene            complement(2127..2999)
                     /locus_tag="PKL17_05745"
     CDS             complement(2127..2999)
                     /locus_tag="PKL17_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNK44264.1"
                     /translation="MTRFLSFRLCQLAVLGLLFFGGSLAAPLPDALAGERILPMGPAE
                     VSSAVGDVSAGRSDRELHAVQKGVRLTTEPWVKTGPGAHAELRFADGTLIRLGERTRI
                     TLFPTERRVWLAQGKVLVVSDRMAGGLAVLSELAALLPEGTSYLAEAVAKPDGKSLAS
                     VTVTVIEGAVCACSIAANVPSQAAATRDAMVLPGETMTIVDLWKRPAPKAVSLLQIVS
                     SEKLWMGFETPLPELRWVKENLDQQRRGILGSRNTRLRREIFWKRPPHPPVPPPTQFI
                     EPAKPPQVRYEFPG"
     gene            3096..3848
                     /gene="rph"
                     /locus_tag="PKL17_05750"
     CDS             3096..3848
                     /gene="rph"
                     /locus_tag="PKL17_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNK44265.1"
                     /translation="MRSDGREAGQLRQTVIEVDYLSAPLACTLVRSGSTWVLCTASVD
                     EQVPPFLRPKPSADGQRNQPQSGWVTAEYSMLPGSTGTRSSRAPSGRSKEIERLIGRS
                     LRQAVDLTKLGPRTITIDCDVLQADGGTRTASITGGYVALALAVKRLMAKGLIRENPL
                     KRAIAAVSVGIIDGQAYLDLPYEEDSRAEVDMNVVMAQPQSGGEPAFVEVQGTGEHGT
                     FSRSQLDALLGLAEGGIGTLFALQQAALAAAR"
     gene            3856..4554
                     /locus_tag="PKL17_05755"
     CDS             3856..4554
                     /locus_tag="PKL17_05755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNK44266.1"
                     /translation="MAETPMQPLVLCLATQNRHKVEELLALLLTLAPDLQGQVKLTSL
                     AELGITDEVVEDGETFADNALIKAKAAWLRTGLWSLSDDSGLMVDALCGKPGILSARW
                     GGEPRSDRRNIDKLLSDLHDVPDEKRQAQFVCTLCLYGQSKQSAAPQVVLREGICKGT
                     LRTTLAGTSGFGYDPLFVPTAAELANAGLAAEKYGLTYAELPADDKNRLSHRTRAVLA
                     LLPDLRRLIGQLST"
     gene            4594..4670
                     /locus_tag="PKL17_05760"
     tRNA            4594..4670
                     /locus_tag="PKL17_05760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4628..4630,aa:Pro,seq:tgg)
     gene            4692..4765
                     /locus_tag="PKL17_05765"
     tRNA            4692..4765
                     /locus_tag="PKL17_05765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4726..4728,aa:Arg,seq:tct)
     gene            4792..5613
                     /gene="ccsA"
                     /locus_tag="PKL17_05770"
     CDS             4792..5613
                     /gene="ccsA"
                     /locus_tag="PKL17_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNK44267.1"
                     /translation="MTLALQAVACTLYVVGVAQPLLRSPLKVARLILLLAVFSQVADI
                     AWLCLRGQHPGSSTREAVYFLSWLLTLAYPLLSRKHPAPLIGALLLPMAMTMEIVVRL
                     VPGVTDPGPAAQRQLLASLHIFCATIGTALFGLAAATSVVYLLSERHLKRHMLDAATP
                     RLPLQTLDGWNHQSIAFGLLAFTATMVSGTYWLSTAGPQPGSTAMGAQLSSLFRSPQY
                     GLSLLTWLIFAGLLIGRRVLGLRGRRAAMWTLLGFAMAVSILGVYLLRDLRLPTP"
     gene            5610..6968
                     /gene="hemA"
                     /locus_tag="PKL17_05775"
     CDS             5610..6968
                     /gene="hemA"
                     /locus_tag="PKL17_05775"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNK44268.1"
                     /translation="MSRLFVIGLSHRTAPLTMRESLAVPDSELSDRLQAAASLCGEAM
                     LLSTCNRVELYVSLGDESAVGKLRKLVTQSQPDAERHLYEKQGPEAVGHLFRVASSLD
                     SLVLGEPQILGQLKQAYRSAQEVGTVGQVLGAIVPKAFAVAKRVRSETAIGENPASVA
                     SVAVQLAHQVFGSLHKQPVLLVGAGKMSELVARHLLQAEVHPLWIVNRTLSRAESLAA
                     ELGGEAHPFDKLESLLCRAQVVISSTGARDPVIREELVARVMKARRGRWLVLIDIAVP
                     RDIEPQVGSLDNVYLYDLDALQQVVSHGQAERQREADSARRIVEEELARQSRRERSAD
                     VNPVIRALRSYALDVATAEVARVLPKLGPLTDKQKQLISSLGPSIVNKLLHLPLTSLR
                     QEVERAEGQESPAELGEAIVKLWSLDKQLAQQSEDASKSGVPRELPSPSQDRKDWENS
                     SS"
     gene            6965..7933
                     /gene="hemC"
                     /locus_tag="PKL17_05780"
     CDS             6965..7933
                     /gene="hemC"
                     /locus_tag="PKL17_05780"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNK44269.1"
                     /translation="MNAVRIATRGSKLALWQAEHIRAELCAREPGLAVELLVVKTVGD
                     QVLDRSLSAIGGKGLFTKEVEEALLDGRADLAVHSLKDVPTEETPGLEIAAYPPREQP
                     WDALLLNPSIRHAKGDSAQKLIGLLPSGARVGTSSLRRGSQILALRSDLQIVPLRGNV
                     DTRLRKLLAGELDAAVLAAAGLHRLGLGDHIAAYFSADELLPACGQGALAVQCRKGDT
                     ATRTRLARLDHLPTRQAVLAERRLNQRLGGSCQTPLAAHARLVEGSLGDSELHLTAMV
                     GSLDGRTVLRAAAQGSPQSPFLLAETVAEALLGQGAQALLRSDAPR"
     gene            8090..9031
                     /locus_tag="PKL17_05785"
     CDS             8090..9031
                     /locus_tag="PKL17_05785"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNK44270.1"
                     /translation="MAHIRPEKHIWLDGKLVPWNESHVHFLTHSLHYGLAAFEGIRAY
                     AQQTPPGTGAIFRLSEHTDRLFDSCKLCLIDVPYTREQINQACIETMQANGMQAAYLR
                     PLVFLGDGSMGLGAMDPPVRVGVAAYVWGRYLGDEGIAKGIRAKISSYGRNNMQVGLP
                     KGKICGQYVNSVLAKREVIKAGYDEAIMLDGSGQVAEATGENIFAVIRGQLVTPPLSA
                     AILAGITRDSVLTLARDLGIPTVEAPIARDQLLLADEVFLTGTAAEVTPVREIDDRAI
                     GNGKPGPITTRIQQTFFAATSGQLHPYSRWLTPIPAT"
     gene            9162..10835
                     /locus_tag="PKL17_05790"
     CDS             9162..10835
                     /locus_tag="PKL17_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44271.1"
                     /translation="MLRRLRAPSLLSLLSALSLTALDPAMGHAASVQVDSQIVGQLYE
                     LRTADAGSSDMRLVDRRRLTTYLGLHVSGLGPKDDDGLPGFRNQFAVHFQMRFDADFG
                     DYLCAIGRTSYGAPLGCLGRDKGGRSLSPELSNYKPEILEGYVEGQSLGGWVDLRLGR
                     QIQWDLFDLRGIDGLWVQARTPIYVAAEVFGGLSQNGTLPIDSPLYALDGTSRSVNPS
                     VGDEQQQTDAYQPTVGFALRSYGLRDVQTRLAYRRTFSSTQNLAPAGCQTTPGQTGNA
                     CAPGFGTIEERLAYSLHGRFLQGRLQGFGGFRYDFVSGKLDDGHAGLRAAPQLGHFVL
                     AEYRYVAPTFDGDSIWNVFSSQPYHDVRLAYDGRLRSPSDDSGNRRYGEFALHAHGFA
                     RMFQSTEHKAIGGRGPLTAAVGGSAGVRWQRGMGQIRLDAYGDGGYGGLRAGSDLAGR
                     ITLWRNRLGFEGRLLYMYFADDVQTENHSHMMGVQLGARWAIINGLLLHILAENNVDR
                     FYNSQLRLMAMLDLSYLLGPGGGGIPPAGLLQMGLGSYPPLGAQAGVLR"
     gene            10838..12367
                     /locus_tag="PKL17_05795"
     CDS             10838..12367
                     /locus_tag="PKL17_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNK44272.1"
                     /translation="MPCFDHKSARAAVWLTILFGVWSASAAPNQKPAAGSSPAAVAAG
                     AETTVTPPAPPPAPPSPDDEQTKAQSIKLEQLLSVKNLPPAEAVSRGERSRVIYPDQT
                     IPLRFSHKNHLAKDIACDSCHQAALTSVSAKDRLLPTEEDCAVCHPIDRADPYKKTSA
                     APATCATCHVGVPSAAAPGTPAPGKDFEALVPRVVIPDPNLKFNHKQHLDKQIPCSSC
                     HGDLSKVDLATRAQLPTMAQCLGCHDNRMPSSTKAQAQRPTTSPAQVENKAGLLGGRV
                     SRGPSPRCGTCHLQQSDGLLATRFPSGVLAPSGLLRGDVHGMDWKGNHRQPALNDPEY
                     CATCHSQAWCQSCHNGVVRPLDLHGGDYVNRHTIEARRNQPDCGSCHRRQTFCLGCHE
                     RLGVVSHSSLLTSPPVSAFSPGTSRRFHPDGWSSPTAGPSHHAWEAQRNLRTCTSCHR
                     EETCIDCHSAQPGGRYSTGKSPHGQEWLSSGRCQSIQSRNARVCLKCHRDGSPQLQCV
                     N"
     gene            complement(12375..12926)
                     /locus_tag="PKL17_05800"
     CDS             complement(12375..12926)
                     /locus_tag="PKL17_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44273.1"
                     /translation="MPTPQKSAPLSRRSLLGILCTLPLSASCLGKSSSKSETQLPNGI
                     YPAIGDPLDAPPANLPSGQRSIRYDPKLADSASTDPVTFVTVDTKDFIPLILSKAPES
                     QRQQDGRLLVGVALADAYVKPLADFTRAHLGQRIALLLDGEVMSCHKIRTVIEGGQIQ
                     ITRCDDHACEKILSKLVKLGVGK"
     gene            13126..14001
                     /gene="fdhD"
                     /locus_tag="PKL17_05805"
     CDS             13126..14001
                     /gene="fdhD"
                     /locus_tag="PKL17_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNK44274.1"
                     /translation="MESPLQILVRHGAQGSPQPLTITMRTPAETRIQRDEDDADLALG
                     FLVGEGIIHSPSEVLAVESPQGDDSVLVTLAQSVRLPTARLERSFFSTSACGVCGKST
                     LAGLAAEPQEPVQPQIPQIAASDLFSLPEIVRQAQSTFERTGGLHAAALFDERLQLVA
                     LREDVGRHNAVDKLIGSRLRVGALPLHGHLLVVSGRASFELVQKARMACIPIFVAIGA
                     PSSLATEVAKQAGMTLVAFLRQKKLNVYCGVERLRKGNAPLVQDPEPDKEDDEACRLP
                     PSPQPGRRLPMIQAK"
     gene            13941..16250
                     /locus_tag="PKL17_05810"
     CDS             13941..16250
                     /locus_tag="PKL17_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HNK44275.1"
                     /translation="MSTSPVPPTGAQTPDDSSQVKLGDRAAQAGGAASIVSTLHELRR
                     NDTGVVRGVRTLLALNQPEGVDCPGCAWPEAAGERGEIEFCENGAKAIAWETTRRRID
                     AAFFAKHSLADLAAQSDYAIGQLGRLVEPMYLRPGATHYEPIGWDDAYALIAKEAHAL
                     SSPDEAAFYTSGRTSNEAAYLFQLFVRQLGTNNLPDCANLCHESSGVALKETVGVGKG
                     TVQLDDFEQADAIFLLGQNPGTNHPRMMAQLQKAAQRGCEIVAVNPLPEAALLRFSHP
                     QHATELLLGGSPITSLFLPVKIDGDAALLKGIMKELLSEERARPGKVFDWEFVRSHTQ
                     GIEALLDDLDATSWEDVCADSGLPRNVIRQAAEVCLSARRIIACWAMGLTQHKAAVAT
                     IQQVVNLLLLGGHIGRPGAGLCPVRGHSNVQGDRTMGITPKVDPEFLGRMEQRYQFVG
                     PKTPGLDSVGTIEAMAKGQIRLFVSLGGNFLSANPDTEFTARALGRCKLSVHISTKLH
                     RGHLLPGGASLILPCLGRTEQDEQKSGPQFVTVENSMSVVHRTQGVLSPASDKLRSEV
                     AIVCEMAQQVLGSRSVVPWLRLRDNYDLIRDEIEATVLGFSHFNERVRSGDGFVLPNG
                     ARNRSFATPEGRAQFTVAPLPKHELSDGQLLMMTIRTHDQFNTTIYGLNDRYRGVFGG
                     RRVILMNQEDMAERGLSAQMLVDLTSHFGNETRVARRFVVVPYAIPRGCTATYFPETN
                     VLVPLQSRADKSRTPTSKSVVITVAKSEN"
     gene            complement(16247..16600)
                     /locus_tag="PKL17_05815"
     CDS             complement(16247..16600)
                     /locus_tag="PKL17_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNK44276.1"
                     /translation="MKISLASAAVKMPGGYLEITAADFARVSYQGRLIDVREPAEFVG
                     ELGHLPRAELVPLATILEKAAVWNREEPLVLVCRSGARSGRAAQQLVAMGFRHVVNLG
                     GGMLGVNAAGMPTDR"
     gene            complement(16633..17532)
                     /locus_tag="PKL17_05820"
     CDS             complement(16633..17532)
                     /locus_tag="PKL17_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK44277.1"
                     /translation="MRIHTLFDPATYTLTYVVYDADSHDAVVIDPVLDYDPNGSQTSL
                     SSVDKVTAFLKENGLRLRHILETHAHADHITGAQILKARFSSSVVIGRRITEVQSTFK
                     TVFDLPDSFATDGSQFDKLLGDGEVLGAGTLHIAAIETPGHTPACVTYRMGDALFTGD
                     ALFIEDYGTGRCDFPRGSAESLYRSIHDKLYTLPDETRVFVGHDYQPGGRPLRYETTI
                     GQSKQKNIQLRADTTMEQFVSFRQNRDATLSAPRLLFPSVQVNVDAGRLPKAHGNGIR
                     YLQIPLNLKGPSEDDGSPRGAAQ"
     gene            17782..18933
                     /locus_tag="PKL17_05825"
     CDS             17782..18933
                     /locus_tag="PKL17_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNK44278.1"
                     /translation="MKPIQKEANKTLHIHGASLRFRAVHSVHLPLLLGIVLLCGLWPR
                     PVAAQLATCSPSPEHALSLFQDRYSLLIGAFAQVQYAASISDQSLTKSDFTVPRARIC
                     ASGHAVTKRLRYRLMIGRSIQRELEVNDAFVEWEPIDGIALRLGRMKLPIIHEWIESA
                     QPLATVDRALASRLLLPGRDYGARVSGLLLQRHVEYIIGVWNGDGDAATRAADTSPAV
                     VARLALHLTGPYVGAVDFDKSAPAVSLEVGTMWNRWKPPSSTNPAPQVEDILVNASAL
                     FRYAHFDGAAEYMTLIRRDDTKTITTQAGYLRLTYFVPRLRSAATLRGSLVDVRQDKP
                     DRQLELEADVGFFLDRHRLKAVLRYAGLLHLIQSTAEHQLGLQAQIAVY"
     gene            18964..20553
                     /locus_tag="PKL17_05830"
     CDS             18964..20553
                     /locus_tag="PKL17_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44279.1"
                     /translation="MNPQTPPQDATTVPESAKAESSASTPRWVFWLWGLLLIASVVPI
                     WFVRYQPLPDHAEHLAAAAILHHYRDTGFEFSRYYAPSLGLVPYWGYYFSVLLFAFLV
                     GVDIANRIVLSLYVTGLPIGFALLAKQFGRSPHLGLFLFPLVWNFNFLIGFTPFCIGL
                     LLIPYALILYDRFCLHPNWATALAAIVGGVITFFAHLLPWGMYIGAAGLVGLCHSERT
                     LRRLLARFSVWLVPVLVGGYVMLHGRGLAMGKVGRGLSWARRSFGENVRVFYDFVWNG
                     CVGHEDELLIGILICTFVALFVTAPKSHKWQLRDLRAEACFLVAVVAYFVLPRSILQP
                     EYWWGVNIRFATMACAFAALLIRGPIVGWRRYLLVPVAISGLGFAIDTTHHFRQAARF
                     AEGFDELAELPGHGTRVLMLLSGARHDPEVRQNYLQCYYEMYQVYHGGYMPWNFDGGF
                     PISYRVRYPAPNWRMMDFDWNKHARYYDYLFVFEGSGGDPRHFFAGHEQQVELAGQRG
                     KWTLWRRKEARVDLDNDIPKR"
     gene            complement(20617..21513)
                     /locus_tag="PKL17_05835"
     CDS             complement(20617..21513)
                     /locus_tag="PKL17_05835"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HNK44280.1"
                     /translation="MAFDIDRFVDLSKAVDTSDLDWDYIKRVGVTADEARILRYMADV
                     ESHTIIYLRDVLSGHTASDPEITQFMACWVYEETHHGRALDKFLTTCGYPPEDNRYSK
                     VLAKMGFQEYIEAFLTVNIPKLTPHFSATHMSWGAVDEMMAASAYTQLAYYTSNKELS
                     KLLLRMSKDERRHQSFYYQQAEKRLQHPLAQALCATALRVFWNPVGMGVGEDTTLGFI
                     AALLYDDERGREDLARMDKLMAKLPGLGWFNRVSLEVGKAIEMFRAREPERAQQIYEK
                     KQLVARERAMMPEDPDFALTPA"
     gene            22039..22446
                     /locus_tag="PKL17_05840"
     CDS             22039..22446
                     /locus_tag="PKL17_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HNK44281.1"
                     /translation="MKRWVCSVSGLLLTAVLSLASPPSASADVGPAPSCPSGTHSAYL
                     YGRRCVKDGYHLVQGPDGQVSEAPDVPAAPPSKDPTPSSPEPTKTPDPPRQVGKSGGC
                     SAADSDIPMLGTTLLAAGLLGLALALRRRRENS"
     gene            22461..23612
                     /locus_tag="PKL17_05845"
     CDS             22461..23612
                     /locus_tag="PKL17_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNK44282.1"
                     /translation="MFEKTPSHCAQVRLLAVRFAISLGIVLGLLLLGNPGHAAPLKVG
                     LTAKSTPCVFNPDGQWRGSFYETWNEISVAANLPFEVVNIPNFKQLLQAGQEGQVDVA
                     VSCVNMTAERLSKYRFSVPVQEDGVSVMVRKEPFHIWTPVVRTLFSLDLLELLGGILA
                     FILAMALVIWKIEKYATQESTLKTGKLRTFSKLFQILLSGPGTNVIANSARSNTLIGV
                     TYFVRVIAASVLVSFVSVNIIKKSTQDVPSAINGIQDLAGKTVSVGTGSVSEHWVNSY
                     NAQLHPSDQKGQIHIKQIDSLQQSCDALRDGQVDAVIADHTQVQYYLTKVNPRAPFQI
                     VIRNVHRQSQGLIFSPQLPPETALQINQAIARLKENGTVDNIKKRWVPE"
     gene            23650..24402
                     /locus_tag="PKL17_05850"
     CDS             23650..24402
                     /locus_tag="PKL17_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44283.1"
                     /translation="MMHRPSNIFSRFRCQSLAFFVAVGGFLFTQGGCAEPPSQPDPDA
                     QALPLTADSWALYTIMPGAHSASISMASPGNPISLFTTVSGRDYRFRFGPSAAYVITK
                     PTQPEDQLDFNKLPGFSDCGTLDLSVNGAMFGWRWRLDLTPNVLEVYAYANNASKHLW
                     STGPLLTLDADDLRSDTPLRYRVWIDGAQYRFAVSGTMRGRAVSATTTLPRACAGTNP
                     SGIKWASGLYFGGTSTAPSKITGYVSEIPFAP"
     gene            complement(24491..24838)
                     /locus_tag="PKL17_05855"
     CDS             complement(24491..24838)
                     /locus_tag="PKL17_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44284.1"
                     /translation="MATPKKRAPSPEKTPEAVEVKAACQPFPWLVTAIPATATAQFAP
                     RNLEVRRREISDRAGLLRRLGYSKADVLSRLLGYQSWEYEPFHTSPLATEVTALVEAV
                     FAPAKGRVTTLQP"
     gene            complement(24886..25911)
                     /locus_tag="PKL17_05860"
     CDS             complement(24886..25911)
                     /locus_tag="PKL17_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNK44285.1"
                     /translation="MANQTRKDVVLLCNPRAGGRWRALAGVLDSPEAKGVRRIVTDHI
                     DDVGAALADVGQRADLVCIYGGDGTIYHVINEMLRIPPNGHPPPRIFLLGGGTMNVTA
                     TKLAMSGTPANNFRAVISAYHAGTLRYRQVPLIAVRTGGQVRYGFTFGMGPLVRLLDR
                     YERGGKGKLAALGLGVQAIAAALSPLRTSFAPLLDQQTATIIADGRPLSQISFAALFA
                     NTTGAVQRLVEPFPSDCPPEHFHFLAYAVSAREMAALVPLLMNGKVPLDLGLLRSPFA
                     AIKTLAGAVTGRGELPRDPRYVNHPAQKLRIETGEAIFTLDGEVLPTDGQLEVTLGPT
                     LSLALHP"
     gene            26032..27549
                     /locus_tag="PKL17_05865"
     CDS             26032..27549
                     /locus_tag="PKL17_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44286.1"
                     /translation="MVATKTPLWVLVGSLLTSFGCGSTWPQVMDPSDIVSLREGTPKI
                     ARVADLGSPHIGNPGPMQPASDGQITVGELVLIEGGGFGKQPTVSLGGRPAEIHWRTD
                     GGGLIVQVPSGVAAGAQPLVVEVGGKRAETTVHVQRLAVVLDGPRGLLHTLKVSGGGG
                     KPPTVEGYGQAVPVAHARSLALSADGAAAYVLWQDKDRDEVAIVDLTAAKGPFLRDHR
                     RLSHRAQTIVAASAAKVVAIIGENQVTVWNVSEANRPSPWPAAKLPEEARGATLFALD
                     PTGTLLAAGFPEQNEVALYDIRPTRTDVVPKEVARATVLPLARQPLLHDLRFSADGET
                     LWTLSGDPSVDKAQQPTQLVALSLGEKEPGQTKRSLSVGKPIEAKDAGAPLALTVGRS
                     RPIASGATIRAVPERSQVLFSTAPRGEGQKAQPGSLFRVAEGGTLKAVLSGGPLVHSV
                     DVSPDAGLAVCAEQGKDSPLAVTAADTEIRSTASFSLGSTTAAADPSSTMLLVQP"
     gene            complement(27546..28358)
                     /locus_tag="PKL17_05870"
     CDS             complement(27546..28358)
                     /locus_tag="PKL17_05870"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNK44287.1"
                     /translation="MLSQLGSPWLHLETCGSTSDEARKLAKAGKPHGTVVTADAQQHG
                     RGRQGRVWYSPPGDNLYLSLILRPPLEPKNVPLLTLCAGLAVLEAAQSVLAEHGLLSS
                     PLLLKWPNDVLAQTPAGLRKVAGILTEMTLVAGRCDFVIVGVGVNVCGRSFPPSVPAT
                     SLLQLVPNEKPPGSVHDFALRLLGCFEPLYLRFLRDGAPWVTAAFSAAATLTAPGKAV
                     SVSTATGTICGESLGIDSDGALIIRKDDGFVERVLAGDLVYDASDPRRTKGG"
     gene            complement(28371..29666)
                     /gene="tyrS"
                     /locus_tag="PKL17_05875"
     CDS             complement(28371..29666)
                     /gene="tyrS"
                     /locus_tag="PKL17_05875"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017020103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNK44288.1"
                     /translation="MTTKNEFLTELKSRGLLHQVSHEEALTAHLASGVRRAYAGFDPT
                     ADSLTIGNFIPIKLLMHWQRAGHQPVVVMGGGTGLIGDPSGKSAERQLMTRETVEHNV
                     SRQRLIFERLLDFSGSTGAQLHNNLDWLGQLSFIEVLRDVGKHFSVNAMIQRDSVRDR
                     LNQREQGISYTEFSYMILQAFDFLHLRRTDGVTVQLGGSDQWGNIVSGSDLIRRILPD
                     GQNESFAVTAPLVTRSDGGKFGKTESGAIWLSPEKTSPYAFYQFWLNTDDGDLSRYLR
                     LFTFFTTVEIADLEALHQKDPGARQAHRALARHMTELLHGPTERAQAEAAAQALFSGD
                     VRALSEKTLREVFAAVPTSTLDKNSITADGLSLVDVLAQTSLCKSKREAREFLQSGSV
                     TVSGRKVGPDDKLVPSDLLHGSTVAIRRGKKNWHILRCE"
     gene            29780..31288
                     /locus_tag="PKL17_05880"
     CDS             29780..31288
                     /locus_tag="PKL17_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44289.1"
                     /translation="MESGHSLRLPPGMRWGLAWVTAVLLIACAVSPSKALAGKPRCRL
                     ERVDLSKFTDGGQIKAVAGVVELEGQVNTERPASAFRLLVNGKPVAKPDKAEPFERAG
                     QELYLVLAVEISALFAPSFEKIKETLREFLESLPPKTKVKLVQFGYEIEPTPVFMAPG
                     ALAPIIDDINPDDQGDVQLLNAIQAGLVALNKVQPGKDKSGEPLPPPRKAIVVLSDGM
                     NQLMDRKTFRRVGDVLRQNGVPLFPIAFSPRDDRGPLLNLGELAKRSFGTFRWAQKDA
                     DLKDQFQNLAEELKKDHVLHFTGKKLSLDALRTAQFALQCGDLKSNTIMQPGVPPPKP
                     STWWKWLLGILLTLVGLWGAAQGALFVLKRKAMKMGALGPPPAQVGVQVPQAAPTQLL
                     GYATMKGNRPLGATLIGIGALAGKRVGIEHTVIVGRSVAGSTNLQVNTDDSLGPSHCE
                     IRREPNGFFLIDYGAPSGSFVNEQRVAGKQLVNDGDLIRLGNATQLKFRLED"
     gene            complement(31277..>32240)
                     /locus_tag="PKL17_05885"
     CDS             complement(31277..>32240)
                     /locus_tag="PKL17_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44290.1"
                     /translation="ASVPYREDEYVNGLTGGSFGKLSLTKVRQSDRYFVLPLLGSDVY
                     PVTAISALRAAFSPTEIISDADRDVAMRVLIGRRDLPAMTNRAILGLAFDDASCNLSF
                     ASVSCGNAVGGPLEEYSGVLGGKLLRPADPNATYDWQEPGDGSQATTIGDLARSWFSG
                     PALDFAEWYFPVRLTLDAVVAATLTMNETDWPVASYGLRAMHGAKMDLPVLALATQLA
                     GKGSGDPRAFDGLRALLTPVPIGPGRPLAGTPRSRPEAFAVMADDKLTHIDPLLGQDR
                     AGSRVQAFYQALADFMKQSSPTGGVVVHVAPTTPTTPMHKRLVF"
BASE COUNT         6240 a         9858 c         9925 g         6217 t
ORIGIN      
        1 cgatccacag tgcaacttga cgtttgggat gttgggcaag cggatgtttg ccctttggga
       61 tcgacccact gaagcttgcg ctcacgccgc cgcctgtctt gagccagtcc gccagggtcg
      121 cgggcttttg cgcccgccgc agcttgccga agtcgccgat cgactgtggc tgctgccggg
      181 tcacctggac ctgagccgat tcgaacagac cctggccgag cagtggccac gggtgatggc
      241 cgcggacaac gaacgggcgc ttgatgtggt gttgtcgctc agcgccctgg cacaccgcgc
      301 cgccgagtca gtcgccgcgg atctggtcct gctagatgtc ggacccagcc tgggcgcaat
      361 caatcactcg gccatgttgg cttgtgacaa cgtcgtcgtg ccgatggcat gggactggtt
      421 tagcctgcaa gccctgctaa acattggccc gagtatcaag gagtggcgag acgactggca
      481 gaccgtcagg gaacgcttcc tgcgcagcca tccctatgca gccttcgcga cgcatcgttt
      541 ccaaccaatc ggctacgtcc ttcagcagcg tatcaaccag ccggacatgg actcgcttgg
      601 ttatgaggcc atcggcttga acgtccagcg tgtcttcaga attcacgttc tgcaagaagg
      661 tcgccttcag gatggcgcgg cgacagatcc agacgaacat cggctggcga tggtcgatca
      721 ctacgcggca ctggctccgc tatcgcaact gttttgcaag ccgatgtttg atctaacgcg
      781 ggccgatggt gccgtgggcg ggcaggttcg gatggttcgg gagtgtcggc ggacgattga
      841 agcgctggcg cgcaaaattt gccgccgcat ggacattccg ttgccgctgc cgccgccgta
      901 gcagattggt gccacctggc aggccgcaag cgtgtgcccg cagagaagtc gcccgtttgg
      961 ggtggtttgg ccggcgcatc gactcggtga gctgtttggg ccaggtgacg accgcggcgg
     1021 atcgacccag cgagctgttc gctcggggtg ctttccctgg cccatcgact catcgagcgg
     1081 ttcggttggg gtgatgactg ggccggatcg actcggtgag cggattgggt ctggtcttta
     1141 ccccagccca tcgactcatc gagtcgttcg gcttgggtcc ggaccccaca tggtcgactc
     1201 gtcatcctga ttcgctgtgg tccatacgcg ggggaaacgc tcgatcgtgc cacagtcgtt
     1261 cgtaagtctt gtcgtcgggc agcgcggggc caagcgtccg accgtaatca accacacggg
     1321 ccagcagctc ttcggcggat acagcaaaca gcggccactg ggaagcatac gccgcgatgg
     1381 ccgcaagccg cgctgggctg ttggccgtca cagtctgtag ctgagccgta aggtcgggac
     1441 gcggccctgg ccgatgcgct tgtccatccg cgcgcaccgc cgtccgcgcc accaagtggc
     1501 cgagcaaaaa tcggaggcct ggtggatagc gacgaaaaag cggcagccca cacagatagt
     1561 ctcggtaggc ggctcgctcc atcgccaccg tgccgcgatc ggtgcctgcc agccttggaa
     1621 cggacgtctc tagggctgcg agccgacgag cgacggaaaa acgggacaag ctgtagggca
     1681 agtcctcaaa gtacgcaagc tgtagaccca gttcaaacgc cagggcgcgg gctgcttcat
     1741 ggacaatccg gtgatcgatg tgtccgccga cggccagtgg cgcaaccagt tcagccggtg
     1801 cgcttacgtg ccagcgcagc cgcgcgacca cttcactcgt gatgcccaaa tgcggcggtc
     1861 catacggcat aaacagcccc agactgccgt ggatttcggg gcggtccggg gcatcaaaca
     1921 gatccagcga caggtatttg catccgagtg cccgtgccgc ctgctcatct tccgcgcgcc
     1981 gggtggcggc gctttctccg ctgacgaaca ccgataggag cgtgacatcg gccccggcgg
     2041 ccacgtcggc aagcagcgac cctgcgcacg acagcgccac atcatcggga tgcggactta
     2101 ggtaaaagac gcgccgcgcc accacgctag cctgggaact catagcgaac ctggggcggc
     2161 ttggccggct caataaactg cgtgggcggc ggtacgggtg gatgcggagg acgcttccag
     2221 aaaatctcgc gccgcagcct ggtgtttcga ctgcccaaaa ttccgcgccg ttgttggtcg
     2281 aggttttctt tgacccagcg cagctcgggc aagggggttt caaagcccat ccacagcttt
     2341 tcactcgata cgatctgcaa aaggctgacg gcctttggcg cgggccgctt ccatagatcg
     2401 acaatggtca tggtctcgcc cggcagcacc atcgcatcac gggtcgctgc cgcctggctt
     2461 ggcacattcg ctgcgatcga gcaggcacag accgcccctt caatgaccgt cacggtcaca
     2521 ctggccagcg attttccatc gggctttgcc accgcctcgg ccagataact ggtgccttcg
     2581 ggcagcagcg cggccagctc cgatagcacc gcgagcccac cggccatccg atcagacacc
     2641 accagcacct tgccctgcgc aagccacacc cgtcgctcgg tgggaaacag cgtgatgcgg
     2701 gtccgttcac ccaggcgaat cagcgtccca tctgcaaagc gcagctccgc gtgggcaccg
     2761 ggccccgtct tgacccacgg ttcagtggtc agccggacgc ctttttgcac cgcgtgcagc
     2821 tcacgatcgg atcgccctgc cgacacatcc cccactgccg agctcacttc ggcggggccc
     2881 atcggcaaaa tccgctctcc ggccaacgca tcggggagtg gcgcagcaag cgaaccgcca
     2941 aaaaacaaaa gcccaaggac agcaagctgg cagagccgaa acgatagaaa gcgtgtcacg
     3001 acgatctcaa cctctaacat acgtgacggc gcgcgccgag cactcttcac ttcgaaggct
     3061 tgacccctgg cgacggtttt gacatcgtcc gcgccatgcg aagcgatgga cgagaggcgg
     3121 ggcagctccg ccagacggtg attgaggtgg attacttgag cgcgccgctg gcttgtacgc
     3181 tggtgcgcag cggatcgact tgggtgctgt gtacggcgtc ggtggatgag caagtaccgc
     3241 cgtttttgcg gcccaagccg tcagccgatg gtcagcgcaa ccaaccgcag agcggctggg
     3301 tcacggcgga atacagcatg ctgcccggct cgacgggaac ccgaagctcg cgggcgccgt
     3361 caggacgcag caaagagatt gagcggctga ttggtcgatc gctccggcag gcggtcgatc
     3421 tgaccaagct gggcccgcgg acgatcacca tcgactgcga tgtcctgcaa gccgacggtg
     3481 gcacccggac tgcatcgatc acaggtggct atgtcgcgct ggcgctggcg gtcaagcggc
     3541 tgatggccaa ggggctgatt cgcgaaaacc cgctcaagcg ggcgattgcg gcagtgtccg
     3601 tcgggatcat tgatggccag gcgtacctcg atctgccgta tgaagaagac agccgcgcgg
     3661 aagtcgacat gaacgtggtg atggcgcagc cccagtccgg cggcgaaccc gcgtttgtcg
     3721 aagtccaagg cacgggcgaa cacggcacct tcagccgcag ccagctcgac gcactgcttg
     3781 gtctggccga aggcggtatc gggacgctct ttgcccttca gcaagcggct ctcgcggcgg
     3841 ctcggtagaa cgtccatggc tgaaaccccg atgcagccgc tggtcttgtg cctggcgacg
     3901 cagaaccggc acaaggtcga agagctgctg gcactgctgc tgacgctggc acccgaccta
     3961 cagggacaag tcaagctgac ctctctggca gagcttggga tcaccgacga ggtggtggaa
     4021 gacggcgaga cctttgccga caacgcgctt atcaaagcca aggccgcatg gttgcggact
     4081 ggcctgtggt cgctgtcgga cgacagtgga cttatggtgg atgcgctgtg tggcaaaccg
     4141 gggattctct cggcgcgctg gggtggtgaa ccacgcagtg accgtcgcaa catcgacaag
     4201 cttttgtctg atctgcacga tgtgcccgac gaaaaacggc aagcccagtt cgtctgtacg
     4261 ctgtgcttgt atggacagtc caagcagtcg gcggcacccc aagtcgtgct gcgcgaaggc
     4321 atctgcaaag ggacactgcg gacgaccttg gcgggcacga gcggctttgg ttacgacccg
     4381 ctttttgtcc cgaccgcggc tgaacttgcg aacgcggggc ttgcagcgga aaaatacggc
     4441 ctgacctatg cggagctacc tgccgacgac aaaaatcgcc tttcgcatcg aacccgagcc
     4501 gttcttgcgc tcttgcccga cctgcggcgg ttgattgggc agcttagtac ttgattgctg
     4561 cgccaggttg gcgtagaact cgttgtcagt cctcggggcg tagcgcagtc cggtagcgcg
     4621 cctgctttgg gagcaggatg tcggaggttc gaatcctctc gccccgacca cagggtcccg
     4681 tcacgggttc tgcgcccgta gctcagtcgg atagagcaac ggccttctaa gccgtgggcc
     4741 gcaggttcga ttcctgccgg gcgcgcttca tctatgcccc cactctttcc ggtgacgtta
     4801 gccttgcagg cggtggcctg taccctctac gtcgtggggg tcgcgcagcc gcttttgcgt
     4861 tcacccctga aagtggcgcg cctgatcctg cttctggcgg tgttttcgca ggtcgctgac
     4921 atcgcatggc tttgtctgcg tggccagcat cccggttcgt cgacgcgcga agcggtgtat
     4981 ttcctgtcgt ggctgctcac gctggcgtat ccacttctgt cgcgcaagca ccctgcaccg
     5041 cttatcggtg ccttgctcct accgatggcg atgacgatgg agatcgtcgt gcgcctggtg
     5101 ccaggggtga ctgatccagg accggcggcg caaaggcagc ttcttgccag tctgcacatc
     5161 ttttgtgcaa cgatcggaac cgctttgttt ggtctcgcgg cagcaacgag cgtggtctat
     5221 ctactaagtg aacggcacct caagcgtcac atgctcgatg ccgcgacgcc gcgacttccg
     5281 cttcagacgc tggatggctg gaaccaccag agcatcgcgt ttgggctttt ggcgttcacg
     5341 gcaaccatgg tgtcgggaac gtactggctt agcacggcgg ggccgcagcc cggtagcacc
     5401 gcgatgggcg cacagctttc gagcctgttt cggtcgccgc agtatgggct gtcgcttctg
     5461 acctggctta tatttgccgg tcttttgatc ggacggcgcg tgctgggtct gcgcgggcgg
     5521 cgggcggcga tgtggacgct gctgggtttt gcgatggcgg tcagcatctt gggtgtgtat
     5581 ctgcttcgcg atctgaggtt gccgacgccg tgagtcgcct gtttgtcatt gggctttcgc
     5641 atcgcacggc accgctgacc atgcgagagt ccctggcggt gcccgattcc gagctgtcgg
     5701 acaggctgca agcggcagct tcgctgtgcg gcgaggcaat gcttctgtcg acctgcaacc
     5761 gcgtcgagct gtacgtatct ttgggtgacg aatcagcggt gggcaagctc cgcaagctgg
     5821 tcacgcagtc tcagccggac gccgagcggc acctgtacga aaagcaggga cccgaagcgg
     5881 tcggacacct gttccgcgtg gcgtcgagcc ttgactcact ggtgcttggc gaaccgcaga
     5941 ttctgggaca gctcaagcag gcctatcgat cggcgcaaga ggtcggcacc gtcgggcaag
     6001 ttctgggggc gatcgtcccc aaggcgttcg cggtggccaa gcgggtgcgc agcgagacgg
     6061 ccattgggga aaatccagcc tcggtggcgt ctgtcgcggt gcagcttgcc catcaggttt
     6121 tcggcagcct gcacaaacag ccggtgctgc tggtaggcgc cggcaagatg tcagagcttg
     6181 tggctcggca ccttttgcaa gccgaagtcc atccgctgtg gatcgtaaac cgcacgctgt
     6241 cccgcgcaga gagtctggcg gcggagcttg gcggtgaagc acacccgttt gacaagctgg
     6301 aatcgctttt gtgccgggct caggtggtca taagctcgac gggggcgcgc gatccagtga
     6361 tccgcgagga gctggttgcg cgggtgatga aggcgcggcg gggtcgctgg cttgtgctga
     6421 tcgacatcgc ggtgccacgg gacatcgaac cgcaggtcgg cagcctcgac aacgtctatc
     6481 tgtacgacct ggatgcgctg cagcaagtgg tgtcgcatgg ccaagccgag cggcagcgcg
     6541 aagccgactc agcccgtcga attgtcgaag aggagctggc acggcagagc cgccgcgagc
     6601 gcagtgccga cgtaaatccg gtgatccggg cgctgcgcag ctacgcgctc gatgtggcga
     6661 ccgcagaagt cgcccgcgtc ctgcccaagc tcgggccgct caccgacaaa cagaaacagc
     6721 tcatcagcag cctgggtccg agcatcgtca acaagctttt gcacctgccg cttacgtcgc
     6781 tgcgtcaaga agtggagcgc gccgaaggac aggaatcgcc ggcagagctg ggcgaagcga
     6841 tcgtaaagct gtggtctctc gacaagcaac ttgcccagca gagcgaggac gcttcgaaaa
     6901 gtggcgttcc ccgcgagcta ccaagcccga gccaagaccg caaagattgg gagaactcgt
     6961 cgtcatgaat gccgtgcgta ttgcaacccg cggctccaag ctggcgctgt ggcaggccga
     7021 acacattcgc gccgagctgt gcgctcgtga gccgggcctt gcggtcgaac ttttggtggt
     7081 aaaaaccgtc ggggatcagg tgctcgatcg gtcactgtcg gcgatcggtg gcaagggact
     7141 gtttaccaaa gaagtcgaag aggcgctgct cgatgggcga gccgacttgg cggttcattc
     7201 gctcaaggat gttccgaccg aggaaacgcc cggcctagag attgcggcgt acccaccacg
     7261 cgaacagccg tgggatgcgc tgctattaaa cccgtcgata cggcatgcca aaggcgattc
     7321 ggcgcagaag ctgatcggtc ttttgccatc cggagcacgg gtggggacga gcagtctgcg
     7381 ccgcggcagc cagatccttg cgctgcgcag tgatctacag attgttccgc tgcggggcaa
     7441 cgtcgacacc cgactgcgga agctgcttgc cggggagctt gacgcggcgg tgctggctgc
     7501 ggcaggactt caccgcttgg ggcttggcga tcacatcgcc gcctacttca gcgcagacga
     7561 gcttttgccc gcctgcggcc aaggtgcact cgccgtgcag tgccgtaaag gcgataccgc
     7621 aacgcggacg cggctggccc gactggacca tctgccgacc aggcaagcgg tcttggccga
     7681 gcgccggctg aaccaacgac ttggcgggag ctgccaaacg ccgctggcgg cccatgcccg
     7741 gctggtcgaa ggatcattgg gcgattccga gctgcatctg accgcaatgg tcggttcact
     7801 ggacggtagg acggtgctgc gcgcagccgc gcaaggcagc cctcagtcgc cctttttgct
     7861 ggcagagacc gtcgcagaag ccctgctggg gcaaggcgcg caggcgctgc tccgctctga
     7921 cgcgccgcgt taggcatctg cgtcgcattc acgacgccat ttcacagttg cgcgacggcg
     7981 ttgcctggtg ttaccgattg aacaggcgac aactcgccct ggcagcaacg ttggctgcgc
     8041 ccagtctccg actaccttgg aaaagagaca aaaggcgcaa ggagcatcta tggctcacat
     8101 tcgtcccgaa aaacacatct ggctcgacgg gaaattggtc ccatggaacg agtctcacgt
     8161 tcactttctg acccactcgc tgcactacgg tctggccgcc ttcgagggca tccgggccta
     8221 tgcacaacag accccgccgg gcacgggtgc tattttccgg ctgagtgagc acaccgaccg
     8281 actgttcgat tcgtgcaagc tgtgtctgat cgatgtgccg tatacccgcg agcagataaa
     8341 ccaagcctgc atcgagacga tgcaagccaa cggcatgcag gcagcctatc tgcgtccact
     8401 ggtctttttg ggcgacggct cgatgggcct gggcgcgatg gacccgccgg tccgcgtcgg
     8461 tgttgctgcc tacgtttggg gccgttacct cggggatgaa ggcatcgcca aaggcatccg
     8521 cgccaagatt tcatcgtatg gccgcaacaa catgcaggtg ggtctgccca agggcaaaat
     8581 ctgcggtcag tacgtcaact cggtgctcgc caagcgcgag gtgataaaag ccgggtacga
     8641 cgaagcgatc atgctcgatg gcagtggcca ggtggcggaa gcgacgggcg aaaacatctt
     8701 tgcggtgatt cgtggccagc ttgtcacccc gccgctgtcg gcagcgatcc tggcgggcat
     8761 cacccgcgac tcggtgctga cgttggcgcg tgacttgggc atcccgacgg tcgaagcgcc
     8821 gatcgcacgc gaccaactgc tgctggccga cgaagtgttc ttgaccggca cagcagcgga
     8881 agtgacgccg gttcgtgaga tcgacgatcg cgcgatcgga aatggcaagc cgggtccgat
     8941 caccacgcgg attcagcaga cgttctttgc cgcgacaagt ggacagcttc acccgtactc
     9001 gcgctggctg acgccgatcc cagccaccta aaaccccccg agcgctgcac gcgcgaaagg
     9061 cgaaagcccc ccgacacagg acgtgctgcg accagatggc gcaacaattg accgtaagtc
     9121 ggggatccgc ttacgatgtt tgaaacgtgc cccgctctcg agtgctacgt cgcctccggg
     9181 cgccgagtct gctttcgctt ctgtcggcct tgagtctgac ggcgctggac cctgccatgg
     9241 gccacgccgc cagcgtccag gtggattcgc agatcgtcgg tcaactgtat gaactgcgca
     9301 ccgccgatgc cggcagctcg gacatgcggc tcgtcgaccg gcgccgactg acgacctacc
     9361 tgggactgca cgtaagcggc cttggaccca aagatgacga tgggctgccg ggctttcgca
     9421 accagttcgc cgtccacttt cagatgcgct tcgacgccga ctttggcgac tacctgtgcg
     9481 cgattgggcg gacgtcatac ggcgcaccgc tcggctgcct cggtcgcgac aagggcggac
     9541 gcagcctaag ccccgagctg tcgaactaca aaccggaaat tctcgaagga tacgtcgaag
     9601 gccagagcct gggtggctgg gtggacctgc ggctgggacg acagatccag tgggatctgt
     9661 tcgatctgcg cggcatcgac ggactgtggg tgcaggcacg gacgcccatc tacgtcgccg
     9721 cggaagtgtt cggaggcctg tcgcaaaacg gcacgctacc gatcgactcg ccgctgtatg
     9781 cgctcgatgg gacgagccgc agcgtaaatc cgagcgtcgg cgatgagcag cagcagaccg
     9841 atgcgtatca accgacggtg ggctttgcgc tgcgttcgta tggactgcgc gacgtccaga
     9901 cccggctggc ctatcggcga acgttttcat cgacgcagaa cctagcgcct gcgggctgcc
     9961 agaccacgcc gggacagacc ggcaatgcct gcgcgccggg ctttggcacc atcgaggaac
    10021 ggctcgctta ttcgctgcac ggacgctttt tgcaaggccg gctgcaaggc tttggcggtt
    10081 ttcgttacga ctttgtcagc ggcaagctcg acgacgggca tgccggtctg cgcgccgcac
    10141 cacagcttgg acatttcgtg ctggcggaat atcgctacgt cgcgccgacg ttcgatggcg
    10201 actcgatctg gaacgtgttt tcgtcgcagc catatcacga tgtccgcctg gcgtatgatg
    10261 gacggctgcg cagcccaagc gacgacagcg gcaatcgccg ttacggcgag ttcgcgctgc
    10321 atgcgcatgg ctttgcccgg atgtttcaga gcacggaaca caaagccatc ggtggacgag
    10381 gaccactcac cgcggcagtc gggggcagcg ccggggtgcg ctggcagcga ggcatgggcc
    10441 agattcgact cgatgcctac ggcgacggag gttacggcgg cctgcgcgct ggcagtgact
    10501 tggccggacg gatcacgctg tggcgcaacc gcctggggtt tgaaggtcgg ctgctgtata
    10561 tgtactttgc cgacgacgtg cagaccgaaa atcacagcca catgatgggc gttcagctcg
    10621 gcgcgcgctg ggcgatcata aacggtcttt tgctccacat cctcgccgag aacaacgtcg
    10681 ataggtttta caactctcag cttcgattga tggccatgct ggaccttagc tacctgctcg
    10741 gaccgggcgg tggcggcatt ccgccggctg gtcttctgca aatgggtctg ggaagctatc
    10801 caccccttgg agcgcaagcg ggggtgctgc ggtgaagatg ccttgctttg atcacaaatc
    10861 ggcccgcgct gccgtgtggc tgaccatcct tttcggggtg tggtccgcgt cagcagcccc
    10921 aaaccaaaag cccgccgcag gttcatcgcc cgcggcagtg gcggctggcg cagagacaac
    10981 cgtgacgccg ccggccccac cgccggctcc gccgagtcca gacgacgaac aaaccaaggc
    11041 gcagagtatc aagctggagc agcttctgag cgtgaagaac ctgccccccg ccgaggctgt
    11101 gagccggggc gagcgatcgc gggtgatcta ccccgaccaa acgattccgc tgcgcttttc
    11161 gcacaagaat cacctggcca aagacatcgc ctgcgatagc tgccaccaag ccgcgctcac
    11221 gtcggtgagt gccaaagatc ggctgctgcc gaccgaagaa gactgcgcgg tgtgccaccc
    11281 catcgatcgc gccgatcctt ataagaaaac cagtgcggcc ccggcgacgt gcgcgacctg
    11341 ccacgtcggc gttccgagcg ccgctgcgcc gggcacgccc gcaccaggca aagacttcga
    11401 agcgctggtg ccgcgggtcg tgattcccga tccgaacctc aagttcaatc acaagcagca
    11461 cctcgacaag cagatcccgt gttcgagctg tcacggcgac ttgtcgaaag tggatctggc
    11521 gacgcgggcc cagctaccga cgatggcgca gtgcctgggc tgccatgaca accggatgcc
    11581 gtcgagcacc aaagcacagg cacaacgccc cacaacgagc ccggcacagg tcgaaaacaa
    11641 agccggtctg ctgggcggac gggtatcccg cggtccaagc ccgcgctgcg gcacttgtca
    11701 cctgcaacag tcggatggtc ttttggcgac gcgcttccca agcggcgtgc tggcaccctc
    11761 gggacttttg cgcggggatg tccacgggat ggactggaag ggaaaccacc gtcagccagc
    11821 gctaaacgat ccagagtatt gtgcgacgtg ccacagccag gcgtggtgtc agtcgtgcca
    11881 caacggcgtg gtcaggccgc ttgatctgca tggcggcgac tatgtaaatc gtcacacgat
    11941 cgaagcgcgg cgcaaccagc ccgactgcgg atcatgtcac cgtcgccaga cgttctgcct
    12001 gggctgtcac gagcggcttg gcgtggtatc gcacagctcg cttttgacca gtccgccggt
    12061 cagcgcgttt tcccctggca cttcccgccg tttccacccc gatggctggt ccagtccgac
    12121 ggcaggccct tcgcaccatg cgtgggaagc gcagcgcaac ctgcggacct gtacgtcgtg
    12181 ccatcgcgaa gagacctgca tcgactgcca cagcgcgcag ccaggcggac gctattccac
    12241 cggcaaaagt ccacacggtc aggagtggct cagctcgggt cgctgccagt cgattcagtc
    12301 gcgcaatgcg cgggtctgcc tcaagtgcca ccgcgacggt tcgccgcagc ttcagtgcgt
    12361 aaactgaccc gcggctactt tccgacgccc agcttgacca gcttggagag gattttttcg
    12421 caggcgtgat catcgcagcg ggtaatttgg atctgaccgc cttcaatcac ggtgcggatc
    12481 ttgtgacagc tcatcacctc gccatccagc aaaagggcga ttcgctggcc caggtgtgcc
    12541 cgcgtaaagt cggcaagcgg cttcacatac gcatcggcga gcgccacccc gacgagcagc
    12601 ctgccatctt gctggcgctg cgactctggc gctttgctca aaatgagcgg aatgaagtcc
    12661 ttggtatcga cggtaacgaa ggtcacaggg tcggtcgatg cactatcggc cagctttgga
    12721 tcgtagcgga ttgatcgctg accgctcggc aggttcgctg gtggcgcatc gagcggatcc
    12781 ccgatggctg gatagatccc gtttggcagt tgggtctcgc ttttcgacga gcttttgcca
    12841 agacaggatg ccgaaagcgg cagcgtgcag agaatcccca gcagacttcg acgcgagagc
    12901 ggagcagact tttgcggagt gggcattgtt attagctgcg catgggtcgg cgtccaggca
    12961 agcggcgcgg acaaaaaccc gcacgacttc tcgcttgtca catttgtccg tcgcttgtcg
    13021 aacctgcgga agtattctgg gcgctagatg gcgcgacgta cccagaccac cccagtattt
    13081 caccttgacg gagacgtgat ctcagaacgg caggaagtac tttgcgtgga atcgccgctg
    13141 caaattttgg ttcgccatgg tgcccaaggc agtccgcagc cgctgacgat cacgatgcgc
    13201 acgccagcag agacgcggat acagcgggat gaagacgatg ccgacctggc gctcggtttt
    13261 ctggtcggtg aaggaatcat ccacagtcct tctgaagtgc tggcggtcga gagtccgcaa
    13321 ggggacgaca gcgtacttgt gacactcgcc cagtcggtcc gcctgcccac cgcacgactt
    13381 gagcggagtt ttttttcgac gagtgcctgt ggcgtctgcg gaaaaagcac gctcgccggt
    13441 ctggctgccg aaccccaaga gcccgtgcag ccgcagatcc cgcagatcgc ggcgtcggac
    13501 ctgttttctc tgccagagat cgtgcgccaa gcccagtcga cgtttgaacg gaccggggga
    13561 cttcacgctg cggccctgtt tgatgaacgg ctccagcttg ttgcgctgcg cgaagatgtc
    13621 ggtcgccaca atgcggtcga caagctgatt ggatcgcggc tgcgggtcgg ggccctgccg
    13681 ctgcacgggc atctgctggt ggtgtcggga cgcgccagct ttgagcttgt gcaaaaagcc
    13741 cgcatggcct gcattccgat ctttgtcgcg attggcgcac catcgagctt ggcgaccgaa
    13801 gtggcgaagc aagcgggcat gacgctggtg gcttttttgc gccagaagaa actgaacgtc
    13861 tattgtggcg tcgagcggct gcgcaaaggt aacgcgccgc ttgtgcaaga cccagaacca
    13921 gacaaagagg atgatgaggc atgtcgactt cccccgtccc cccaaccggg gcgcagactc
    13981 ccgatgattc aagccaagta aagcttggcg atcgggccgc gcaagctggc ggtgccgcat
    14041 ccattgtgtc gacgctgcac gagcttcggc gcaatgacac gggagtggtc cgcggcgtgc
    14101 gaacgctctt ggcgctaaac caaccggaag gcgtcgactg tccaggctgc gcgtggccgg
    14161 aagcggcagg cgaacgcggc gaaatcgagt tctgtgaaaa cggtgccaaa gcgatcgctt
    14221 gggaaaccac ccgtcggcgc attgatgcag cgttctttgc aaaacacagc ctggcggatc
    14281 tggccgcaca gagtgactac gccattggac agcttggccg cctggtcgag ccgatgtacc
    14341 taagaccggg cgcgacccac tacgaaccga tcgggtggga cgatgcctat gcacttatcg
    14401 cgaaagaggc acacgcgctt tcttcccccg atgaagcagc gttttacacg tcgggacgga
    14461 ccagcaacga agcggcgtat ctgttccagt tgtttgtccg ccagcttggc acaaacaacc
    14521 tgccagactg cgcaaacctg tgtcacgaat cgagcggcgt cgcgctcaag gaaacggtag
    14581 gcgtcggcaa aggaaccgtg cagcttgacg attttgagca ggccgacgcg atctttttgc
    14641 tgggtcaaaa tccggggaca aaccatccgc ggatgatggc gcagctacaa aaagcggcgc
    14701 agcgaggctg tgaaatcgtc gcggtgaatc cgcttccgga agcggcgctt ctgcgctttt
    14761 cccatcccca gcatgcgacg gagcttctgc tgggtggatc gccgattacg agcctgtttc
    14821 tgcctgtcaa aattgatggg gatgcggcgc tcctcaaagg gatcatgaag gagcttctgt
    14881 ccgaagagcg cgcacgtccg ggcaaagtct tcgattggga atttgtccga tcgcacaccc
    14941 agggaatcga agcgctgctc gatgatctgg atgcgacatc ttgggaagat gtttgcgcgg
    15001 acagcgggct gccacggaac gtgattcgcc aggctgccga agtctgcctt tcggcgcggc
    15061 ggatcatcgc ctgctgggcc atgggactga cccagcacaa agcggcggtg gcgacgattc
    15121 agcaggtcgt aaaccttctg cttctgggag gtcacatcgg tcgaccagga gcgggtcttt
    15181 gtccagtgcg cgggcactcg aatgtgcagg gggatcggac catgggaatc acgcccaaag
    15241 tcgatccaga attccttggc cggatggagc agcgctatca gttcgtcggc ccgaaaacac
    15301 cggggctcga ttcggtgggg accatcgaag cgatggccaa gggacagatt cgcttgtttg
    15361 tctctctggg cggaaatttc ctgtcggcca atcctgatac agagttcaca gcgagggcgc
    15421 ttggccgctg caagctgtcg gtacacatat cgaccaagct gcaccgcgga catcttttgc
    15481 caggcggtgc gtcacttatc ctgccgtgcc ttggtcgcac cgaacaggat gaacaaaaga
    15541 gtggtccgca gtttgtcacc gtcgaaaatt caatgagcgt agtccaccga acgcagggag
    15601 tcctaagccc agcatcggac aagctgcgca gcgaagtggc aattgtctgt gaaatggcgc
    15661 aacaagtgct cggcagtcgc agcgtggtgc cgtggttgcg tctgcgcgac aactacgatc
    15721 tgatccgcga tgagatcgaa gcgaccgtct tgggcttttc ccatttcaac gagcgcgtcc
    15781 gcagcgggga tggttttgtg ctaccaaacg gagcgcgcaa tcgcagcttt gccacgccgg
    15841 aaggccgggc tcagtttacc gtcgcgcccc ttcccaaaca cgagctttcc gacgggcagc
    15901 tcctgatgat gacgatccgg acgcacgatc agttcaacac cacgatttat ggattaaacg
    15961 atcggtatcg tggtgtgttc ggcggtcggc gcgtcatctt gatgaatcaa gaggacatgg
    16021 cagagcgcgg tctgtcggct caaatgctcg tcgatctgac cagtcatttt ggcaacgaga
    16081 cgcgggtggc gcggcgtttt gtggtggttc cctatgcgat tccgcgcggc tgcacggcga
    16141 cctatttccc agagaccaat gtcctggttc cactgcagag tcgcgcggac aaaagccgca
    16201 caccaacatc caagtctgtg gtcatcaccg tcgccaagag cgaaaactag cggtcggtcg
    16261 gcatccccgc ggcgttcact ccgagcattc cgccgcccag gtttacgaca tgacggaaac
    16321 ccatcgcgac aagctgctgg gccgcccgcc ccgaacgtgc gccggatcga cagaccagca
    16381 cgagcggttc ctcgcgattc cagaccgctg ctttttccaa aatggtggcc agcggtacaa
    16441 gctctgcgcg gggcagatga cccagctcgc cgacgaactc ggctggctcg cgcacgtcga
    16501 taagtcgtcc ctgataagac accctggcaa agtctgcagc agtgatttcc agataaccac
    16561 ctggcatctt caccgcggcg ctggcaagtg agattttcat gggaggagtc ctttgcccaa
    16621 agaggaaagt agctactgtg ccgccccgcg cggagagccg tcgtcttccg acggaccctt
    16681 gaggtttaaa ggaatctgta gatagcggat tccgtttccg tgcgctttgg gaagccgccc
    16741 cgcatcgaca ttcacttgca cgctgggaaa cagcagccgc ggagccgaca gcgtcgcatc
    16801 gcggttttgc cggaaggaca caaactgttc catcgtggta tcggcgcgca gctgaatgtt
    16861 cttctgtttg ctctgcccga tggtggtttc atagcgaagc ggacgcccgc ccggctggta
    16921 gtcgtgaccg acgaagacgc gcgtttcatc cggaagggtg tagagcttgt cgtgaatcga
    16981 tcgatacagc gattcagcac tgccgcgcgg gaagtcacag cgtccggttc cgtagtcttc
    17041 gataaacagc gcatcgccgg taaacagcgc atcccccatc cgatacgtca cacaggcggg
    17101 agtatgtccc ggcgtttcaa tcgccgcgat gtggagcgtt cctgcgccaa ggacttcgcc
    17161 atcaccgagc agcttgtcaa actggctgcc atcggtcgca aagctatccg gaaggtcgaa
    17221 gacggtctta aacgtagact gcacttcggt gatccgccga cctatcacaa cactggaaga
    17281 aaagcgcgcc ttgagaatct gcgccccggt gatgtggtca gcgtgcgcgt gggtttccag
    17341 aatgtggcgc aggcggagtc cgttttcctt gaggaacgcc gtcaccttat cgaccgatga
    17401 aagcgaagtc tgcgatccgt tgggatcata gtcgagcacc ggatcgatca cgaccgcatc
    17461 gtggctgtcg gcgtcgtaga ccacataagt cagcgtgtac gtcgcggggt cgaacaaggt
    17521 gtggatacgc atcgaatgaa ctccaagcgg aagaggttgc tggctggaag ctgccgttgc
    17581 ttccgaaaca agagcaacgt gcatgccatc cactctgccg cagccatctc ctcgcggctt
    17641 ccgtccttct gtcccacttg gtttcaattc tgtttcaagt ctgaaacatc atgaatccag
    17701 ccgcgaacca cgccaacgca gactcgccgc ccaggcacgc ggcatcacgc ttgctgtctt
    17761 tgccgtgcag aggtcgcaac gatgaaacca atccaaaaag aagcaaataa aacgcttcat
    17821 attcacgggg catcactccg tttccgcgcg gtgcattccg tccacttgcc tcttctgctg
    17881 gggattgttt tgctgtgcgg actgtggcct cggcctgtcg cagcgcagct tgcaacctgt
    17941 agcccgagtc cggaacacgc cttgtcgctt ttccaagacc gctattcctt gctgatcggc
    18001 gcgtttgccc aagttcagta tgcggccagc atcagcgatc aatcgctgac caaaagcgac
    18061 ttcacggtgc cgcgcgcgcg catctgtgcg tcgggacatg ccgtgaccaa gcggctgcgc
    18121 tatcgactga tgatcggacg aagcatccag cgtgaactgg aagtaaacga cgcctttgtc
    18181 gagtgggaac ccatagacgg aatcgcgctg cggctgggtc gaatgaagct gccgatcatc
    18241 catgagtgga tcgagtcggc gcagccgctg gcgacggtcg atcgcgcgct ggcatcacga
    18301 ctgctcttgc ccggacggga ctatggggcg cgggtgtccg gactgctttt gcagcggcat
    18361 gtcgaataca tcatcggggt gtggaacggc gacggtgatg cggcgaccag agcggcggat
    18421 acctcgcccg ccgtcgtcgc gcgcctggcg ctgcacctga ccggacccta tgtcggcgcg
    18481 gtggactttg acaaaagcgc gcccgcggtg agcctggaag tcggaacgat gtggaatcgc
    18541 tggaaaccac cgtcgagcac aaacccagcc ccgcaggtcg aagacatcct ggtaaatgca
    18601 agcgcgctgt ttcgttatgc ccacttcgac ggcgcagccg agtacatgac actgatccgc
    18661 cgcgacgata caaagaccat cacgacgcag gccggctacc tgcggctgac gtactttgtg
    18721 ccgcgcttgc gatcggcggc gacgctgcgt ggatcgctcg tcgatgttcg gcaggacaaa
    18781 cccgaccggc agctggagct ggaagcagac gtgggtttct ttttggatcg tcaccgcttg
    18841 aaggcggtgc tgcgctacgc tggtctgctc catctgattc agtcgacagc tgaacaccaa
    18901 cttggcctgc aagcccagat cgccgtctac taaccctgtg ttagcctatc gccaccgctt
    18961 gcgatgaacc cacaaacacc gccccaggat gcgacgacgg ttccagaatc ggccaaagcc
    19021 gaatcgtccg catcaacacc gcgctgggtg ttttggctgt ggggattgtt gctgattgcg
    19081 tcggtggttc cgatttggtt cgtgcgatat cagccactgc ctgatcacgc agaacatctg
    19141 gccgccgcag cgatactgca ccattatcgc gacaccggtt tcgagttttc ccgttactat
    19201 gcaccgagct tgggcttggt tccttattgg ggatattatt tttccgtctt gttatttgcc
    19261 tttctggtgg gcgtcgatat tgccaaccgg atagtcttgt ccttgtatgt gacagggctg
    19321 ccgattggtt tcgctttgtt ggccaagcag tttgggcgga gcccgcacct tggactgttt
    19381 ttgtttccgc tggtttggaa ttttaacttc ctgattggat ttaccccttt ctgcatcggt
    19441 ctgctactca ttccctatgc actcattctt tatgatcgtt tctgtctaca tccaaactgg
    19501 gcaacagcgc tggctgccat cgtgggtggt gtgatcacgt tttttgcgca tttgttgccg
    19561 tggggaatgt acattggcgc agccgggctg gttggattgt gtcattcgga acgcacgctg
    19621 cgacgcctgc tggcgcgttt ttcagtctgg ctggttccag tcttggtcgg cggctatgtg
    19681 atgctgcatg gccgtgggct cgcgatgggc aaagtcggac ggggtctgtc gtgggccagg
    19741 cgcagcttcg gcgaaaacgt ccgcgtcttt tacgacttcg tctggaacgg ctgcgtcggc
    19801 catgaagacg agctgctgat tggcatcctg atttgcactt tcgttgcgct gtttgtcaca
    19861 gcacccaaat cgcacaaatg gcagcttcgt gacctgcgcg cggaagcgtg tttcctggtt
    19921 gcagtggttg cgtattttgt gctgccgcgc tcgattcttc agcccgaata ctggtggggt
    19981 gtaaacatcc gatttgccac catggcttgt gcatttgctg cgcttttgat tcgcggcccc
    20041 atcgtcggct ggcgccgtta tttgttggtt cctgtggcga tatcaggact gggttttgcg
    20101 atcgatacca cacatcattt tagacaagcg gcccgtttcg cagagggctt cgatgaactg
    20161 gcggaacttc ccggtcatgg cacgcgggtg ctgatgcttt tgtcgggggc gcgtcacgat
    20221 ccagaagtcc gtcaaaacta tcttcagtgc tattacgaaa tgtatcaggt gtatcatggc
    20281 ggatatatgc catggaattt cgacggaggt ttccccattt cctatcgtgt gcgctatcca
    20341 gcacccaact ggcgaatgat ggatttcgac tggaacaagc acgcgcgcta ttacgattac
    20401 ctgtttgtct tcgagggcag cggcggcgat ccgcgccact tttttgcagg tcacgaacaa
    20461 caagtcgagc tggccgggca gcggggcaag tggacgctct ggcgacgaaa agaagcgcgg
    20521 gtcgatctgg acaacgacat tccaaagcgg taagcgccgc gccaaaaaat cggcagcgtc
    20581 gtcgatccgc ttgcgccgtg gcccggcaat gacacgctag gctggcgtca gcgcgaagtc
    20641 gggatcttcc ggcatcatcg cccgctcacg cgcgacaagc tgcttctttt cgtaaatctg
    20701 ctgggcacgc tccggctcgc gggcgcggaa catctcgatg gccttgccga cctctaggga
    20761 cacccgattg aaccagccca ggcccggcag cttcgccatc agcttgtcca tccgcgccag
    20821 gtcttcgcga ccccgctcgt cgtcgtacaa tagcgccgcg atgaagccca aggtggtgtc
    20881 ttcgccgacg cccattccga ccggattcca gaacacgcgc agtgccgtcg cacagagcgc
    20941 ctgcgcgagc ggatgctgga gccgcttttc cgcctgctga tagtagaacg actggtggcg
    21001 acgctcgtcc ttggacatcc gcagaagcag cttcgacagc tctttgttgc tggtgtagta
    21061 cgcaagctgc gtatatgccg atgctgccat catttcgtcg acggcacccc acgacatgtg
    21121 ggtggcggaa aagtgcggcg tcagcttggg aatgttcacc gtcaaaaatg cctcgatgta
    21181 ttcctgaaag cccatcttcg ccagcacttt gctgtagcgg ttgtcctcgg gcggatagcc
    21241 gcaggtggtc aggaacttgt cgagcgcccg tccgtggtgc gtttcctcgt agacccagca
    21301 ggccataaac tgggtgattt cgggatcgct ggcggtgtgg cccgacagca cgtcacgcag
    21361 atagatgatg gtgtggcttt ccacatcggc catgtagcgg aggatgcggg cttcatcggc
    21421 ggtgacgcca acccgtttga tgtagtccca gtcgagatcg ctggtgtcta ctgctttgga
    21481 gagatcaacg aaccgatcga tatcaaatgc cattgctacg ttcctttggc tacgcagtga
    21541 tttttccgtt ttgatcttta tcacgcagag aaaacaaaag gtgcgccata tcacatcgcg
    21601 cggtcgtcta cacaatgtgt cagccgtcgg aaagtccgac cttttggata gagccaaagt
    21661 gcgcaaggtg atcatgtgtc gcagtgggcc acggtcgacg ggagtctgcg gtaagacaca
    21721 agcgctgctg gcttatgtgc acacaaattc ggaaagccgc atgcctctat ttgcgcaacg
    21781 aacccaagca ctacatcgaa ttcatgtaga tatcttcccc aaaggaagaa ttaaggctta
    21841 gtgactgtct gacgatacgt gctatcgcga ccgccctatc agacagacag tcaaagacgc
    21901 ctccgcgcgc gtgtgttcac ccgcaagacc gcccattcac ggatcgcagc tcgcacgccg
    21961 agcggttgtg cgggccaaaa atccgttcgt tccgcaggct tgtgagacga gcttgccagt
    22021 cgtgatagga tgcgggccat gaaacgatgg gtgtgctctg tttccgggct gctgttgacg
    22081 gctgtgctgt cgctggcatc gcccccttct gcatccgccg acgttgggcc ggcaccgtct
    22141 tgcccaagcg gaacccacag cgcgtatctg tacggcaggc gctgcgtcaa agatggctac
    22201 cacttggtcc aaggtccaga tggccaagtc agcgaagctc ccgacgttcc cgcagctccc
    22261 ccaagcaaag atcccacgcc aagctcgccc gaacccacaa agacgcccga tccgccccgc
    22321 caagtgggca aaagtggcgg ctgttccgca gcagacagtg acattccgat gctggggact
    22381 acgcttttgg ctgcgggtct gctcggtctg gccttggcgc ttcgtcgtcg ccgtgagaac
    22441 tcctaacagg attggttccc gtgtttgaaa agacaccttc ccattgtgcg caagtccgcc
    22501 tgctcgctgt ccgtttcgca atatcccttg gcatcgtcct cgggctccta ttgcttggga
    22561 atcccggcca cgccgccccc ctcaaggtcg gtttgaccgc caaatcgacc ccctgtgtgt
    22621 tcaatcccga tgggcagtgg cgcggatcat tttatgaaac gtggaacgaa atttccgtcg
    22681 ctgcgaatct tccgtttgaa gtggtgaaca ttcccaactt caagcagcta ttgcaggccg
    22741 gacaagaggg tcaggtcgat gtggcggtca gctgcgtcaa catgacggcg gaacggctta
    22801 gcaagtatcg cttcagcgtt ccggttcagg aagacggcgt atcggtgatg gttcgcaagg
    22861 aaccgtttca catctggacg ccagtggtac ggacgctgtt ttctctcgat ctgctagagc
    22921 ttctgggcgg catcctcgcg ttcatcttgg cgatggctct tgtcatctgg aaaatcgaaa
    22981 agtacgcgac ccaggagtcc accctaaaga ccggcaagct gcgcacgttt tcgaagctgt
    23041 ttcagattct gctcagcggt cctggcacaa acgtgatcgc caattctgcc cgcagcaaca
    23101 ccctgatcgg agtgacgtac tttgtgcggg tgatcgcagc ttcggttctg gtcagctttg
    23161 tatcggtgaa catcatcaag aaatcgacgc aagacgttcc cagcgcgata aacggaatcc
    23221 aggatctggc cggcaagacg gtatctgtgg gcaccggcag cgtcagcgaa cactgggtca
    23281 acagctacaa cgcgcagctt catccaagcg atcaaaaagg tcagattcac atcaagcaga
    23341 tcgacagcct gcagcagtct tgtgatgcac tgcgcgatgg acaagtcgat gcagtgattg
    23401 ccgaccatac ccaggttcag tactacctaa ccaaagtcaa tccgcgcgca ccgttccaga
    23461 ttgtgatccg caacgtccat cgccaatcac aggggctgat cttttcgccg cagctaccgc
    23521 cggaaaccgc gctccagatc aaccaggcga ttgcgcgact caaggaaaac ggaacggtcg
    23581 acaacatcaa aaaacgctgg gttccggaat agctcccgct tctgtctggg ctgtgcatac
    23641 actgcggaca tgatgcacag accatcgaac attttttcac gctttcgctg tcaaagcctg
    23701 gctttcttcg tcgctgtggg cggattccta ttcacccaag gcggctgtgc agaaccccca
    23761 tcacaaccag accccgatgc gcaagcgctg ccgcttaccg cagattcgtg ggcgctttac
    23821 accatcatgc ccggtgccca cagcgcgagc atcagcatgg cgtctccggg caacccgatc
    23881 agcttgttta cgacggtatc tggccgcgac taccgcttcc gctttggccc ctctgcggcc
    23941 tatgtgatca ccaaaccaac ccagccagaa gatcagctcg actttaacaa gctgccagga
    24001 ttttctgatt gtggaacgct cgatctgtcg gtaaacggcg cgatgtttgg ctggcgctgg
    24061 cggctggatc tgacgccgaa tgtgctcgaa gtgtacgcct acgcaaacaa cgcgagcaag
    24121 cacctgtggt cgacgggacc gcttcttacg ctggatgccg acgacctccg cagtgacacg
    24181 ccgctgcgct accgcgtgtg gatagatggc gcgcagtatc gcttcgcggt cagcggcacg
    24241 atgcgaggcc gcgcagtcag cgcgacgacc accctgcccc gcgcgtgcgc cggcacaaat
    24301 cccagcggaa tcaagtgggc atccggtctg tatttcggtg gaacatcgac tgcgccaagc
    24361 aaaatcaccg gctacgtgtc cgaaattccc tttgcgccct aacggtcgag gcagaaacgc
    24421 tgcgccaaac agagtgcgcg ccaatatgag tcattcaaaa tgattcatat tggacgcagt
    24481 ccaaactcgg ctacggctga agtgtggtca cgcgaccttt ggccggagcg aacacggctt
    24541 caacaagcgc agtgacttcc gtggccaacg gcgacgtgtg gaacggctcg tattcccagc
    24601 tttggtagcc gagaagtcgg ctcagcacat ctgctttgct gtaaccaagg cgacgcagaa
    24661 gtccagcgcg gtcgctgatc tctcgccgcc gcacttcgag gttccgtggc gcaaactgag
    24721 ccgtcgcggt cgcgggaatt gccgtcacaa gccacggaaa cggctgacaa gccgccttga
    24781 cttcgacggc ttctggcgtc ttttcaggac tgggagcgcg tttttttggg gtagccatgg
    24841 gcgaatccta atacgttttt cgcccggcag ttgcagttgt gttgtttacg gatgcagtgc
    24901 cagcgaaagc gtcggaccca gcgtcacttc aagctggccg tccgtcggca gaacctctcc
    24961 gtcgagggta aagatcgctt cgccggtctc gatccgcagc ttttgcgccg gatggttcac
    25021 gtagcgcgga tcacgcggca gctcgccccg cccggtcacc gcccccgcca gggtttttat
    25081 cgccgcaaac ggactgcgca aaagacccag atcgagcggc acttttccat tcatgagcaa
    25141 aggcacgagc gccgccattt cccgcgccga caccgcatac gccaaaaagt gaaagtgttc
    25201 gggtggacaa tcggatggaa acggctcgac cagacgctgc actgcgcccg tggtgttggc
    25261 aaacagggcc gcaaaactga tctgtgaaag cggtcgtcca tcggcaatga tcgtcgcagt
    25321 ctgctggtcg agcagcggcg caaacgaagt ccgcagtgga ctgagcgccg ccgcaatcgc
    25381 ttgcaccccc agcccaagcg ccgccagctt gcctttgccg ccgcgttcat agcgatcgag
    25441 cagccgcaca agcggcccca ttccgaaggt gaagccgtag cgaacctggc ccccggtgcg
    25501 caccgcgata agcggcacct gccgatagcg cagcgtcccc gcgtgatagg ccgaaatcac
    25561 cgcacgaaag ttgttcgctg gcgttccgct catcgccagc ttggtcgccg tcacgttcat
    25621 cgtcccgcca ccgagcagga agatccgcgg cggcggatgc ccattgggcg gaatccgcag
    25681 catttcgttg atgacgtgat agatcgtgcc gtcgccgcca tagatacaga cgaggtcagc
    25741 acgctgcccg acatccgcca gcgctgcgcc cacgtcgtcg atgtgatcgg tgacgatgcg
    25801 ccgcacacct ttggcttcgg gactgtcgag aaccccggcg agcgcccgcc agcgcccccc
    25861 tgcccgcgga ttgcacagga gcaccacgtc cttgcgtgtc tggtttgcca tgccgcacct
    25921 accgtaccca aaaagccggg ctgcggggaa cttccttgcg gcccccttca ccgagcgatg
    25981 gcgcggtcgc tgcgttgaca agcggggcga cacgttggaa tgcttggcgc catggtggct
    26041 acaaagactc cgctgtgggt gctggtcggt tcgcttctca cctctttcgg ttgtggctcg
    26101 acgtggccgc aggtcatgga cccaagtgac atcgtgtcgc tgcgcgaagg cacgccaaag
    26161 attgcgcgcg tcgctgattt gggttcgcca cacattggca accccggacc gatgcagccg
    26221 gcatccgatg gtcaaatcac cgtcggagag cttgtgctga tcgagggcgg cggatttggc
    26281 aaacaaccca cggtttcgct cggtggtcgt ccggcagaaa ttcactggcg caccgatggc
    26341 ggcggactga ttgtccaggt gccgagcggc gtcgctgcgg gcgcacagcc gctcgtcgtc
    26401 gaagtgggcg gcaagcgggc cgaaacgacc gtccacgtcc agcgacttgc ggtggtgctc
    26461 gatggaccgc gcggtctttt gcatacgctc aaagtcagtg gcggcggcgg aaaacccccg
    26521 acggtcgaag gctatggcca agcggtgccg gttgcgcatg cacgcagctt ggcgctaagc
    26581 gccgacggag ccgcagccta tgtgctctgg caagacaagg atcgcgatga agtggcgatc
    26641 gtcgatttga ccgcagccaa aggaccattt ctacgcgacc acagacggct ttcgcaccgg
    26701 gcgcagacga tcgtcgcggc ctcggcagcc aaagtggtgg cgatcatcgg ggaaaaccag
    26761 gtcacagtgt ggaatgtcag cgaagccaac cgtccgtcgc catggccggc ggccaagctg
    26821 ccagaagaag cacgcggagc gacactgttt gcgctggacc cgacggggac actcttggcg
    26881 gcgggatttc ccgaacagaa cgaagtcgcg ctgtacgaca tccggccaac gcggaccgac
    26941 gttgtgccca aagaagtggc gcgggcgacg gtgctaccgc tggctcggca gccgctgctc
    27001 cacgatctgc gcttttctgc cgatggcgag acgctgtgga cgctaagcgg cgatccatcc
    27061 gtcgacaaag cccagcagcc gacccagctt gtggcgctgt ctttgggcga aaaagagccg
    27121 ggccaaacca agcggtcgct gtctgtcggc aagccgatcg aagcaaaaga cgccggggca
    27181 ccgctcgcgc tgacggtggg acgcagccga ccgatcgcca gcggagcgac catccgggcg
    27241 gtgcccgagc gatcgcaggt gctgttttcg acggctccgc gtggcgaagg gcaaaaagcg
    27301 cagcccggat cgctgtttcg cgtcgctgaa ggcggcacgc tcaaagccgt gctaagcggt
    27361 ggtccgctgg ttcacagcgt cgacgtaagt cccgacgcag gtttggcggt ttgtgccgag
    27421 cagggcaaag acagcccgct cgcggtcacc gctgccgaca cggagattcg cagcaccgcg
    27481 tcgttttcgc tcggatcgac gaccgctgct gccgacccgt ccagcacgat gcttctggtt
    27541 cagccctagc cgccttttgt ccgacgcgga tcgcttgcgt cgtagaccag atcgccagcc
    27601 aagacgcgct cgacgaagcc gtcgtctttg cgaatgatga gcgcgccatc cgaatcaatg
    27661 cccagcgatt ccccacaaat cgtcccggtc gccgtcgaaa ccgacaccgc tttgccaggt
    27721 gcggtcagcg tcgcggcagc ggaaaatgcc gcagtcaccc acggcgcacc gtcgcgcaaa
    27781 aaacgcaggt agagcggctc gaaacagcca agcagccgca gcgcaaagtc atgcaccgat
    27841 ccaggcggct tctcattggg gacaagctgc aaaagacttg tcgccggcac agacggtgga
    27901 aaactgcgac cacagacgtt gacgcccacc ccgacgatca caaagtcaca gcgcccggcg
    27961 acaagggtca tctcggtcaa aatccccgcc actttgcgca gtcccgcggg cgtctgagcc
    28021 aaaacgtcgt taggccattt cagaagcagc ggcgacgaca aaagcccatg ctccgccagc
    28081 accgactggg ccgcttcgag caccgccaac ccagcacaca gggtcaaaag cggtacgttt
    28141 tttggctcga gcggcggacg caagatcagc gacaggtaca gattgtcccc cggcggcgaa
    28201 taccacaccc ggccttgccg accccggcca tgctgctgcg cgtcggcagt caccaccgtt
    28261 ccgtgcggtt tgccggcttt ggccagcttg cgcgcctcgt cgctggtcga tccgcacgtt
    28321 tccaggtgca gccatggact gcctagctgc gacagcatga ggcgacccga ctactcacag
    28381 cggaggatgt gccagttctt tttgccgcga cggatggcga cggtgctgcc gtgaagcaga
    28441 tcgcttggca ccaatttgtc gtcgggacca accttgcgcc ccgacaccgt gaccgatccc
    28501 gactgaagaa actcgcgcgc ttcccgcttc gacttgcaca gcgaagtctg cgccagcaca
    28561 tcgacgagag agaggccatc tgcggtgatg ctgtttttgt cgagcgtgct cgtcggaacc
    28621 gccgcaaaga cctcgcgcag cgtcttttcg gacagcgccc gcacatcacc ggaaaacagc
    28681 gcctgggctg ccgcttcggc ctgcgcccgc tcggtcggac cgtgcaaaag ctcggtcatg
    28741 tgccgagcca acgcgcgatg ggcctgccgc gcccccggat ctttttggtg cagcgcttcc
    28801 agatcggcaa tctcgacggt ggtaaaaaac gtgaacagtc gcagatagcg cgaaagatcg
    28861 ccgtcgtcgg tgttcagcca aaactgatag aacgcatagg gactggtctt ttccggcgac
    28921 agccagatcg cccccgattc ggtcttgcca aactttccgc cgtcgctgcg cgtcacaagc
    28981 ggcgccgtca ccgcgaagct ttcattctgt ccgtcgggca aaatccgccg gatcagatcg
    29041 ctgcccgaca cgatgttgcc ccactgatcg ctgccgccaa gctgcaccgt cacgccgtct
    29101 gtccgtcgca gatgcagaaa gtcaaaggct tgcaggatca tgtacgaaaa ctcggtgtag
    29161 ctgatgcctt gctcacgctg gtttaaccgg tcgcgcaccg agtctcgctg gatcatcgcg
    29221 tttaccgaaa agtgcttgcc gacgtcgcgc agcacttcga taaacgacaa ctgtcccagc
    29281 cagtcgaggt tgttgtgcag ctgggccccc gtcgatccgg aaaaatcgag cagccgttca
    29341 aaaatcagcc gctgccggga cacgttgtgc tcgacggttt cgcgggtcat aagctgccgc
    29401 tctgccgatt ttcccgacgg atcaccgata agtccggtgc cgccccccat caccaccacc
    29461 ggctgatgtc cggcgcgctg ccagtgcatc agcagcttga tcgggatgaa gttgccgatg
    29521 gtcagcgagt ccgccgtcgg atcaaagccg gcataggcac gacgcacgcc gctcgccaag
    29581 tgcgcggtca gggcttcctc gtggctcact tggtgcagca ggccgcggct ttttagctcc
    29641 gtcagaaatt cgtttttcgt cgtcacggca gcaccatagc cgacccgtgc gccgcagtaa
    29701 accgcgtaag cccagcgcag agcggcttgc gactcgggag gttgagagtc acaggccgca
    29761 tcggatacga tgtaacgtga tggaaagtgg ccattccctg cggctccccc ccggcatgcg
    29821 ctggggcctt gcgtgggtca ccgcggtgct gctcatcgcc tgtgcggtgt cgccgagcaa
    29881 ggcgctggcg ggcaagccgc gctgtcggct cgagcgagtt gatctgtcca agtttaccga
    29941 cggtgggcag atcaaagcgg tagccggtgt cgtcgaactc gaaggccagg tcaacaccga
    30001 gcgtcccgcg tcggcgtttc gactgctggt caacggcaag ccggtcgcca agcccgacaa
    30061 agccgaacca ttcgagcgcg ctggacaaga gctgtacctg gtgctcgccg tcgagatttc
    30121 agccctgttt gcgccgtcgt tcgaaaaaat caaagaaacc ctgcgcgagt ttttggaatc
    30181 gctgccgccg aaaaccaaag tcaaattggt gcagtttggc tacgaaatcg agccgacgcc
    30241 ggtgttcatg gcaccgggtg cgctggcgcc gatcatcgat gacataaacc ccgacgatca
    30301 gggtgatgtg cagcttctga acgcgattca agcggggctc gtcgcgctca acaaagtgca
    30361 gccgggcaaa gacaaaagcg gcgagccact gccgccgccg cgcaaagcga ttgtggtgct
    30421 gtcggacggc atgaaccagc tgatggatcg caagacgttt cggcgggtcg gcgacgtgct
    30481 gcgccaaaat ggcgtgccgc tgtttccgat cgcgttttcg ccgcgcgatg accgcggacc
    30541 gctcttgaac ctgggtgagc tggccaagcg atcgtttggc acgttccgct gggcacagaa
    30601 agacgccgac ctaaaggacc agtttcagaa cctcgccgag gagctgaaaa aagaccacgt
    30661 cctgcacttt accggcaaaa aactgagcct cgatgcgctg cggacggcgc agtttgcgct
    30721 tcagtgcggc gatctgaagt cgaacacgat catgcagccg ggcgtgccgc cgccgaagcc
    30781 aagtacgtgg tggaaatggc tcctcggcat tttgcttacg ctcgtcgggc tgtggggtgc
    30841 ggcgcaaggt gccctgtttg tgctcaagcg caaagcgatg aagatgggcg cgctcgggcc
    30901 accaccggcc caagtgggtg tgcaagtgcc gcaggcggcg ccgacgcagc ttctcgggta
    30961 cgcgacgatg aaaggcaaca ggccacttgg cgcgacgctc atcggcatcg gcgcgctggc
    31021 tggcaaacgg gttggcatcg agcacaccgt catcgtcggt cgatccgtcg ccggcagcac
    31081 caacttgcag gtaaacaccg acgacagctt ggggcccagc cactgcgaaa tccgccgcga
    31141 gccgaacgga tttttcctga tcgactacgg cgctccgtcg ggaagtttcg taaacgaaca
    31201 gcgcgtcgcc ggcaagcaac tggtaaacga cggcgatctg attcggctgg gaaacgcgac
    31261 ccagctcaag ttccgactag aagactagcc gcttgtgcat cggcgtcgtg ggcgtcgtcg
    31321 gtgcgacatg aacgaccacg ccgcccgtcg gactgctctg cttcatgaaa tcggcgagcg
    31381 cttggtaaaa tgcctgcacg cgcgagcccg cccgatcttg cccgagcagc ggatcgatgt
    31441 gggtcagctt gtcgtcggcc atcaccgcaa acgcttcggg ccgactgcgc ggtgtccccg
    31501 cgagcggtcg ccctggacca atcggcaccg gggtcaaaag cgcccgaagc ccgtcgaaag
    31561 cccgcggatc gccactgcct ttgccggcaa gctgcgtcgc cagggccagc actggcaagt
    31621 ccatcttcgc tccatgcatc gcgcgcagtc cgtaggaagc caccggccag tcggtttcgt
    31681 tcatcgtcag cgtcgctgcc accactgcgt ccagggtcag ccgcactggg aaataccact
    31741 cggcaaaatc gagcgcagga ccggaaaacc acgaccgcgc caagtcgccg atcgtcgtcg
    31801 cttggctgcc gtcgccgggt tcctgccagt cataggttgc gtttggatcg gcaggacgca
    31861 aaagcttccc accgagcacg ccactgtatt cttcgagcgg accgcccacc gcattgccac
    31921 acgacaccga cgcaaacgag aggttgcagc tcgcgtcgtc aaacgccagg cccaagatcg
    31981 cgcggtttgt catcgccggc agatcgcgac gaccaatcag cacgcgcatc gccacatcgc
    32041 gatcggcatc actgatgatt tccgtcgggg aaaacgccgc ccgcagcgct gaaatcgccg
    32101 tcaccgggta gacatcacta ccgagcagcg gcagcacgaa atagcgatcg ctctgacgga
    32161 ctttggtaag gctcagcttg ccaaacgacc cgccagttag gccatttacg tattcatctt
    32221 cccgataagg caccgacgct
//
LOCUS       DAORGN010000033        31501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_534_length_31501_cov_6.406029, whole genome shotgun sequence.
ACCESSION   DAORGN010000033 DAORGN010000000
VERSION     DAORGN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 31501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31501
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_534_length_31501_cov_6.406029"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKL17_05890"
     CDS             complement(<1..239)
                     /locus_tag="PKL17_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44291.1"
                     /translation="MRLHTDALISYFQQLIQHNEDADIAKSYVKTLQEISGFVDIKSR
                     LPAGPSGRRDLSVGRETLLQFGKLLKDRRLKARLSR"
     gene            complement(575..1951)
                     /locus_tag="PKL17_05895"
     CDS             complement(575..1951)
                     /locus_tag="PKL17_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44292.1"
                     /translation="MACVWAWLCLACLWLFPSPIWAKPDFRSVGQVADSVAVARVTQL
                     LAAAEQALARNALTLTYQLLEEAYRLRGDVDTLYLLGHLAYVERRSVEAFDLFRRYAR
                     EKEGPLGDEHPEVPKVLAEPLPPAGEVLILGERGSLVLVDERLVGTLPLSAPLLLPAG
                     KHQVVLELAARRKQQDLDIHSGSSWLLRSTVDSEDIAASLAPIIVFLPHYRGLDGEAI
                     RQLEQAAEQAAHGSGHILKRREMAMTAAPQLASCLDSLLCQEELGKRNQAEYVLLLHA
                     EHSGLGQQSDWRLHLSVIDVAVGDRAATGTPLCASCTISKAAATIGSTLAHLLEQAQE
                     KPKGNLQVLSVPSRAEVFVGERKLGITPLNRPAWAGRLPLEVRSPGLPPYQGEVLVEP
                     DRSVSLSIDLGEGGSSDADQQKKQTRKPIYKRWWFWAAGGAVLGGISLGLILGLTPRS
                     HQNIDWVK"
     gene            2252..2773
                     /locus_tag="PKL17_05900"
     CDS             2252..2773
                     /locus_tag="PKL17_05900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNK44293.1"
                     /translation="MSIQVASPAPSFKTQALVGREFKTVSLSDYAGKWVLLMFYPMDF
                     TFICPTELVGLNDKHGDFADRDCVVLAASTDTVHSHLGWVKADARLGNLKYPVLADVT
                     KSISRDYGVLIENEGIALRGTFLIDPNGVLRWACVNDGSAGRSIDEMIRVLDALQTDK
                     LCPCNWKKGEATL"
     gene            2928..3536
                     /locus_tag="PKL17_05905"
     CDS             2928..3536
                     /locus_tag="PKL17_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNK44294.1"
                     /translation="MKFSQEELDGARSRVAERFSVQAAPTVLSQIPAEVVRDAALNLA
                     RALDGDSAILARGDRLLIGLGVALAKNGRPGAGLAGWLADAAKVAGKTDEDIEAAAAA
                     ALACATYNGYYKFRSLVSDSKPYESFQPGLRASPFVRSALSRTLVELISLAVSVMNGC
                     TSCVNGHVETVKGGNLPNPDAAIDEAVRISAVVAALAAWDKA"
     gene            3618..4055
                     /locus_tag="PKL17_05910"
     CDS             3618..4055
                     /locus_tag="PKL17_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNK44295.1"
                     /translation="MHDGSMERQQLLHYEEILRNADQFVTVQRRALLKYLLRHRVHPT
                     TAQIASAVGRSGSASLATVYNNLALFAELAIVRTLRSPRGEVHWDIRTDRHHHLVCSD
                     CGHVDDVSEEQVEVSIRAPDIIHRVTHTEAWLLWSCHRCKPTL"
     gene            4227..5228
                     /locus_tag="PKL17_05915"
     CDS             4227..5228
                     /locus_tag="PKL17_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44296.1"
                     /translation="MSALVATEACGLPDDAEFRGAPPQVQHVTIAFPGVSTMSPRLPR
                     EDSTPKNTDTAQYYELTQSMAALLNSYALQLLTVARVMVQLPATTRMGESRTWGPYEP
                     GGADPLTYRLVVNRLGEAVFAYTLSARNQSAGSDNEFLVVLDGSVTKGDESSAAKGRM
                     TVHFDNRRKLVPDSCEQGKIDFEYDSLGDPALLDITLQKVGSQNPLGRKCRQDPPRDG
                     RFHVEHGSDDSGNFLFDVRKNIHGDDANRPLLETVLLRSRWNEKGQGRSDGKIADGEV
                     SIDLRNAGLSERYVSFSQCFGQGGKALYQTASPESLRLLTSLGDPSVCTPKTAAFPD"
     gene            complement(5261..6553)
                     /locus_tag="PKL17_05920"
     CDS             complement(5261..6553)
                     /locus_tag="PKL17_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK44297.1"
                     /translation="MSALGITIILAMVLAAGVFLLFTIINNISVCPPSEVLIFSGRTR
                     RLADGRLVGYRVIRGGRAMRIPLIETKDRMQLTNMAIELSVTNAFSRGGIPLKVHAVA
                     NVKLPSEESLLHNAIERFLGASREGIMRVAKDTLEGNLRGVIAELTPEEINQQKMIFQ
                     QKLIEEADKDLHRLGLLLDNLQIQNISDDVSYLSSVGRVRSARIKKEARIAELQAWAS
                     AAVQKAHNQMNAEISKIDTDTQVAQKENQRRIVEAQTKREAVISEVRGQVQAQLTEAK
                     AQLRAWEARAEQVRRKLEADVISSAQATKSKAEADAKANAASIVAEGRAAAAALVSLS
                     QAYQAGGRSARESLLLQKLLPVFSELTGTMKAIKVDRLTLLGGASGGSGGGTPLGAQV
                     VAVNEQVRAATGVDLVSTVQRAVLRSAQPHIPSPPTGK"
     gene            complement(6550..7899)
                     /locus_tag="PKL17_05925"
     CDS             complement(6550..7899)
                     /locus_tag="PKL17_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK44298.1"
                     /translation="MAQNPLGLWALASRALDEPPPQAFSDDARLAGVVMLAAAFVVGL
                     LVFVWMLRQFLYICRPNELLVVSGKSHRTSTGEATTFTVVLSGTHFRIPFLQTVDRMD
                     LRLIPIELSMPKALSSGGIPLDIHAIANVKISSDPRFVYNAVERFLGLPRETIWQTAK
                     QTLEGSLRDVISQLTPEQVNQDRIEFANQLVQVSNQIFNKMGLQLDTLKIQRVEDEAG
                     YLVNLGRAQIANAVRDAENAENQANQEIAQSEAAARQLAEVAQKDAEIGVAQKRNQLR
                     QVVGQLEGQAQAVEREATAAAEQARAEAEQELQTVRKDLNQRKLFAEIVLPAEADQKA
                     QSLLSEGSAAPRREQGLAAAEAMRLLSEALQAAGPQARELFVLSQLDTLVAQVASKVK
                     DLSVREIQIVDTGDGTSLAQVAAAYPAIVTEVLSTLKGLTGVDIKNLLTPPTEGGSR"
     gene            complement(7902..8705)
                     /locus_tag="PKL17_05930"
     CDS             complement(7902..8705)
                     /locus_tag="PKL17_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44299.1"
                     /translation="MLYLYVFAAVAGGLLVLVSLFGGGQHDNQSDGDDQGQAHGDAQA
                     GQAHGGHLDAAAGHGDGHGDGHGDAAGHGDGHGHGDGHGDGHGLSTHDQGHAHDGDGM
                     ASGVPTALLWLMSIQVWTYLLAFGGLTGLLLRTVAKVPEPICGMSALLVGVSTAFAAR
                     AAMQRLSVTRESGTLVEEKMVGSTAEVLIPAPPGGCGKVRLEVAGQTVDLLAKASDGG
                     PLLAGSLVTILDVRDSQAEVSVDRKDEREQQAKQRGAIQAGRSAQEKGQ"
     gene            8837..9967
                     /locus_tag="PKL17_05935"
     CDS             8837..9967
                     /locus_tag="PKL17_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK44300.1"
                     /translation="MAPGSKYLSTMIERIYLDNIRTFVNFEWKPAKLALILGGNGSGK
                     TALLATLRAIRRFVGGQYSSEEAFPATSLTRWEDKRTDQTVEIDLRVGTDLFGYRLVI
                     AQNPKEPGANRVKHESLRKNGELCIQREENKLELAYEVGWPQLSIKPTRSAMDIGDNR
                     TDAPSSVFQSAVWHLWFLQPDARQMTARLADSPQGESSAWLRHNFSNFADWYLRILQT
                     RPKSVFSALRTLEEIIPGLQELHADSGWLYARFESEGKTASFRFDELSDGQRALIALH
                     LLRHVAATPGSVLALDEPDNYVALAEIQPWLLDVERAALKSGGPQVWLISHHPEVINL
                     LAPEYGVRFFRDGMGPTRTAPFQSAQQLSASETVARGWDNEP"
     gene            9957..10574
                     /locus_tag="PKL17_05940"
     CDS             9957..10574
                     /locus_tag="PKL17_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44301.1"
                     /translation="MSRSVSILLLCEDKQARVFVESVLEQIDPSLLRRLRVVPFPVTG
                     RTGTAQGLVDGYKVYPCGSMHIRENYPQLVSQRRQNAGRFRAMGCRLIVQIDVDNSEK
                     SPRSVADRRAELVRACEAQKITPPAANEPVAHLIPRRNIETWIVFGNSGQPVDEGTEY
                     PHLTGHESDCEPAARRFADECKTNQPSALAPPSWTFGLSELRKVL"
     gene            10886..11551
                     /locus_tag="PKL17_05945"
     CDS             10886..11551
                     /locus_tag="PKL17_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44302.1"
                     /translation="MPQIRDERTGTDELIRDGSYHYAALLADPDAAHLAPLVKKRLDA
                     LSAAQADMHKAELGRIEVQALAERAEYQHDRRQSVLELEVLAAVGRSRKNPAYQEVYP
                     HGLSGLLSLSGEEQEHATVGLLQKLKQHHPDLWKTHSKELTKLAQDATAAEKALKKAQ
                     VEESRAFLDEQAARAELVRQLRKNEGALISLYPGERGAIRLYYRQPKKRTSPAASPAP
                     HPA"
     gene            11609..12559
                     /locus_tag="PKL17_05950"
     CDS             11609..12559
                     /locus_tag="PKL17_05950"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HNK44303.1"
                     /translation="MRLGSALAFGLLPILACGTGCHKKGAKQASLVLYTGYGSSAPGM
                     LYGRVQKGPPDPTPEPGQSKIQRIQETLQALDASALPHAAVDIKVGDHLLSATSDDRG
                     YLEVTLPAKLAPPAVPVEIHLRTPGYDAPPLAGSIPVYDGTAGLAVVSDVDDTVLDSQ
                     ITDKSKMVKNALLRSTWELVTFPDAPQVLSQFGKDKPVFYLSGSPWGFQPRIASFFAR
                     VGLPMGTIILKRFSSEPLLDQMGFKYPHLLDIAKALPEKKLILFGDSGEKDPEIYARL
                     RSEQPSRVEQIYIHLVTKEAPDSPRFTGMKVFQHWSELRP"
     gene            complement(12626..15226)
                     /locus_tag="PKL17_05955"
     CDS             complement(12626..15226)
                     /locus_tag="PKL17_05955"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNK44304.1"
                     /translation="MAKFASRPWLRPLLPLFSVALLSCSHNDAPVPDDAPPGPCAAQG
                     LSEKSRLPAGDAQGHRDPLGAKAAKQARASRISDPAWIRQADTARHPVRTGDYLLIND
                     KIAVYIEAPGVSDGYQPFGGEILSLDTVGPDGKPSGNSLYGETIMALGRQVLKAETVS
                     VINDGSDGNAAVIRTSGVLTDLPFLEFLGSVFADNFYLPAAIDYILLPGAESVKLRLH
                     ILNTRDEELPLYRYQLFVLFQSSRGQLFTEKAGYGDLNGDQQWLGFDSGDSSFAMHFL
                     SGSYTPLLGRSGSIIGRGAGLTAKACSPLSTDYVEFIVGGPHSDGLSEAVRRVLGQPA
                     YRAVTGTVTDTSGAPQAGAYVHALRSGGGGYIARTQTNARGEYTLHLPAEPVELSATQ
                     YGQPPSVKKIVAPTENTANLKMEATGTLSVTAVEKGSGVALPVRVQVIPQAGPPSFPD
                     SYGVRDEAGGRSVQEFAVSGSTKLAIPAGKQRVIISRGPEWDLFDQTVTVESGKTTEV
                     RAELLHTVDSQGVMCADFHIHSYFSADSSDDVEYKVKGAIADGLEIPISSEHEYIIDF
                     QPIVRRLGLTRWAFGMPSEEFTTFAWGHFGVIPITPRPDEPNRGAVQWYGKKPPEVFH
                     TIAQLPEKPVLIVNHPRSDSFGGYFSSVSFDRATVSGDPDLYSDEYGALEVFNDSDFE
                     SNRNAIVQDWFALLNAGKKVWAVGSSDSHGLRTSPVGYPRTCLRFGHDDPTRLSAETV
                     RDVLRAGKAVVSGGLYMTVKGPDGEGPGEQIRSAGRPVQFQVVVQSPSWLTAKRLETI
                     VDGETIATEELRETVTPQGHRYETTVNVMGKPGRSQHWVVFHASAGGLDLTPVHPGRR
                     PFAVSNPIFF"
     gene            complement(15358..16572)
                     /locus_tag="PKL17_05960"
     CDS             complement(15358..16572)
                     /locus_tag="PKL17_05960"
                     /inference="COORDINATES: protein motif:HMM:NF020269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HNK44305.1"
                     /translation="MKNKKLLCRSALVLPLGLLLSAVGCGGYQSSDGTAVAALNLQLK
                     GDFSGGTVVSQPAGIDCGTTCASTFAVGQSITLTVNLSDSTDFVGWDGDCTGSSPTCT
                     LSLDKGKNVGATFKSKTNRANVDLTIGGAGGGLVTSMPFGIQCSGGTCSGGFDKGTML
                     TLTAAPDTTSYFTGWTGGCTGSARTCTITLTSDVQISANFGNPASCEQLRADNPTLTD
                     GARKLFVGGDKTKSWDAYCVMSVTPALSYLPLTNVAATQNFSQIPTGGGVTGTTVKTN
                     YTRLLIDPSTLKIDTNDRRFAASTGAAAKGTTNITAMPYATAMACNGTTTAGTANIDL
                     TGTKFAVATGALVLGGTTATGMATPTPDGQVRVINLTGNGSAAACGWFAPTGASTPIN
                     SAGSPLQLVYFP"
     gene            complement(16799..17452)
                     /locus_tag="PKL17_05965"
     CDS             complement(16799..17452)
                     /locus_tag="PKL17_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44306.1"
                     /translation="MRAFRLWLLGPLIGLGFGGCGPSLADRVDALQQLLEAQSEQIQR
                     LRLEIGDGIGMAMCTPELSQLIEDVQRECEAQTETCTTKQIRPAVIAADPEHKGRFLK
                     LMSHLRHEVFYFRDGASQVIPFRAERLHKLLRSALLRNTQFLIVSSPDSGEQEAHRRA
                     RAMEQILVERGIPRAKIRRWVYAFPQLKEEVERSVDLPGLGETRDLHRGVWVFRSDC"
     gene            17623..18339
                     /locus_tag="PKL17_05970"
     CDS             17623..18339
                     /locus_tag="PKL17_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44307.1"
                     /translation="MSAVTLLLGESAAHADLGSLGSTTIGLALSAGFQTDPRTCHVAG
                     DTRNAPGTGCAMLVAGIEGDFLWRGRIGMLLGLSSTAGQANVPTTSMPGGDAPPAFPD
                     RVSVVAGLDVRPLGFFINREDRSYKARLFHGLRMGIGPSLEIVRTALDSSIAASSRSG
                     AVASSLIGMHALLDGEIPLVASAANSLSLRVSVRLLYVPKISLNDGTVLSQPFDPTSS
                     SEPQGYGLRTQLLFGLAYYL"
     gene            18436..19044
                     /locus_tag="PKL17_05975"
     CDS             18436..19044
                     /locus_tag="PKL17_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44308.1"
                     /translation="MELRTFSFLDILQPQLTGFLQTVAQGFLPLERQASLIVEIAPGI
                     AVNQLTDVALKQTRVIPGMQIVERAYGLLELHSFDQGEVRSAGAAILSAMGKDESARL
                     KPKVLSEQTITGIEGYHSQLINRFRHGDMIGEKQTLYTLELQPAGYAALCANEAEKAA
                     PVHLLEVVTFGAVGRLWLGGYEAEIEAAREAIHAALAGLSGR"
     gene            19048..19743
                     /gene="nth"
                     /locus_tag="PKL17_05980"
     CDS             19048..19743
                     /gene="nth"
                     /locus_tag="PKL17_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNK44309.1"
                     /translation="MQPGAAVENKTTTRRTRKSETARIADILDELDRLYPDAHCELVY
                     KSPFELLIAVILSAQCTDDRVNMITPTLFARFPDPAALAQADLEELEKIIYSTGFYRN
                     KAKNIKACAKKLVEKFGGKVPQSMPDLLSLPGVARKTANVVLGTAFGIPSGVVVDTHI
                     GRLSQRLGMTKQDDPEKIELDLQKLWPKDRWIMSGHRLIWHGRRVCFARKPSCATCTL
                     RPVCPAKEAKELP"
     gene            complement(19766..20203)
                     /locus_tag="PKL17_05985"
     CDS             complement(19766..20203)
                     /locus_tag="PKL17_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNK44310.1"
                     /translation="MTLDNLVGLSLEKIEMDEAAVRRLLDAAALSLRDAQLDGLSPEG
                     RFDLAYKAIMQTANAALQANGYRTLTSKPGHHQTMVQSLARTIGLEYPNVILLDKLRK
                     QRNDIDYTGEVVSKELSQTCLREASTLYTRVCDHIAERKNAVR"
     gene            complement(20190..20768)
                     /locus_tag="PKL17_05990"
     CDS             complement(20190..20768)
                     /locus_tag="PKL17_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010655397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNK44311.1"
                     /translation="MSLASVLFPGYRRRVLGLLLLHPSQSFHVREIARLTGSSTGSLT
                     RELSKLADAGLLIRERQGNQVRYAANRGALVFEELASILRKTSGLADVLAAALLPLTT
                     RIRAAFVFGSMATGKATMQSDVDILIIGDVEFGEAVECLFPAQATIGREINPKVFSAP
                     AWQALLHKGGAFICDVLAKPKLFVIGGPDDLG"
     gene            complement(20844..22346)
                     /gene="gspN"
                     /locus_tag="PKL17_05995"
     CDS             complement(20844..22346)
                     /gene="gspN"
                     /locus_tag="PKL17_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HNK44312.1"
                     /translation="MRRQLIRLLRRLRKTNRVVTVLLYAVFGTLTFFVSLSLSLPIDK
                     IKDRIERELSQDQGPPISATGGFGIGTGMDVAIGELDLHVLRPGVSATDVRLKPRKVP
                     GSDAAAAKNLRPIVIDRLDARVKPLDAAFGTKSGSLSVEAFGGELTAEGSQGDDGVQV
                     VANLHDVTLLRLSALSGLLPLPMLGTLALSLHFKAPNQKPATPARAGAAPLLNPPPPR
                     LDVAKAVGDLEIKLGQATIGDGKAKLVVPGDAFLSQGITFPKLRLGDVIGKVTIERGR
                     ANIVDVHAKSPDVELWVDGYLELRDPIQLSDARLYVRFKPSPQLTAREPTMELVVNSQ
                     SQGKRADGAIGFAITGSLANPKAKAAKDPPDGVVLRAGTLGQVSPAARPSFSPGTPPA
                     PSPSPPPMMPSQAAPQPQAIPSPPPPSSDTVVVPPPPQNPPPIQQAIPSPPPPPPNPL
                     ASAGPPPGTLHVPPPNQPTPSEAAAPSQQPHSPSPESAPKPEASPVPAPQ"
     gene            complement(22346..22990)
                     /locus_tag="PKL17_06000"
     CDS             complement(22346..22990)
                     /locus_tag="PKL17_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44313.1"
                     /translation="MSSSEPTIIGKVPGFARVREFLDGLAPRERALLMTLVITVLGLG
                     MLGGAVYLYDRIDTLEEQTDAMQRALRDIAKKRGPYLQARARAQELDRRIGTTPLQLS
                     GFLEGLAKENSIEIRETNPRTPEPMGKKYMQQSVDLRISKVGLESLLKFMHKIETYPS
                     NLVLVTQLNVRSRDDKHQEFEVDMTVSTYEHAPKTAAKDKKGDGKTDSPAEKEP"
     gene            complement(23037..24836)
                     /gene="pilM"
                     /locus_tag="PKL17_06005"
     CDS             complement(23037..24836)
                     /gene="pilM"
                     /locus_tag="PKL17_06005"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNK44314.1"
                     /translation="MSTILGIDLGSYSVKVARIEQGFRTVHLASIEEERVPEPGEGTP
                     LPPKAVDRKHESSLNSEMAAADASPAIDLTTDSLLDRQMRALLTILGRQKIRAESCAV
                     GISEDVTLRLIEMPLSDAKKVAQTLPFELAGQLMEDLDGHVVDQLPADVPAGSGASLW
                     LAACAKQQSVELRLGSLGPLGIDPRVCGSTALATAALWIQSKQGSMPQKDLPLWVVDL
                     GHRMTHVLALAAHPKRPGQVLTQFARTIPRGGQQITEAIAKHIGMGLIAAEKLKHDNG
                     LSDFSNPRVAKVIRDSLRPLLRDLKQTLGTYVSRFGSEPKALFLTGGTAQLHGVQDLL
                     AEELGVPVQPLLPPEGATWLGPGVREMPAGESMTFGEANASRTYVSSTALVHRWPTGT
                     AAVGLGLGAALGGPNFNFRKGELAFRTDYAMLREKAPLLVGFAVSLVACVGLWAYASL
                     KQLEKESDRLRLQLISESTSLFEKPETDGHKVSAELAAALSQDKAQGQSIPQVSALDL
                     VEDISAAAPSSNAAGDAKLDVIELHIRPKKTDIKATCASAQYVDDLATSLGKIPCFRK
                     VQKGKVLTLKNTGPDGKPVEVKQFSLEIETTCP"
     gene            complement(24852..26390)
                     /locus_tag="PKL17_06010"
     CDS             complement(24852..26390)
                     /locus_tag="PKL17_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNK44315.1"
                     /translation="MDRKDCGSLDVASVPTGKPDRKRRQRGMAILTVLVSLALLGATT
                     ADLAYNAQVEMEAAANSRDMLRAEYLATSGIELAKLLVAVQGGMQNMLQSLPAEFRDA
                     IVITDFAGFLAQAFGGDKEARDGLGAMVGIDLGSVEGMGTPKGTSFDLAITSEEGKFP
                     INCGGGYNPNKEAQRSLYLLLDSLVRPPRFDRLFQVPDRDGVVINREDVPTNIIDWTD
                     VDVSRYSPVGAGSASEETYDKGRDRYEPHNQYFDTIEEMIQVRGVSEDLWAYFGDFFT
                     VYGTQDCKVLASAMEPTAWPLLAAMIAASAVDRTAVFDPNTSFVAQQLAGMLKSGLPM
                     LKSMSGELNKLGSCKPDPRCPNATPQTTTNKTTSTTTKTATPPPASDAIETLSNVICS
                     SAIEKLPQMAESLSSMTGMSSPPTLTTPLKPIALCPGMLARFLRDRPTAAAGAQGGSS
                     AGKSPRRFFRIDATGSVQRSAQKNTQVHIRGVWDSQSMNMNPVCVNHPSCFKNGTWLY
                     YRLD"
     gene            complement(26415..27104)
                     /locus_tag="PKL17_06015"
     CDS             complement(26415..27104)
                     /locus_tag="PKL17_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNK44316.1"
                     /translation="MKRRQAGFTLIEVMVSIGILSSITVLLWATSQGTFRTKKAVESK
                     MARYRVARLAMDRMLRDVQMAYLSDNNVVGTEQTPRTYFDGVNRPDIDELRFSYFGHQ
                     RLYAESKEGDTAAVAYFGLRDKSDGGKFNLWRKETRRLQADRIDNANIPGEAEIIADD
                     VVRLELSYYEPVRKEWLDTWRTTQADGMPNKLPSRVRVKLVIHDERGEELPLQSEVRI
                     AMFQKLDTKPN"
     gene            complement(27101..27676)
                     /locus_tag="PKL17_06020"
     CDS             complement(27101..27676)
                     /locus_tag="PKL17_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNK44317.1"
                     /translation="MRRKQAGFTLLEVMIAMAILAVSLVSLYLTTGRAIRVSMHARAM
                     TQATFLCRQKMSEIQNEFVTEGFKDDAGVKEDRGDFQDPAFKQFRYVTIIEKIRLPAT
                     DQMQSAATKLLQDKQSAASKNKTASASSSSSSGNLGGSMGGFLGPVKDMLEQGIRRVT
                     VRIEWDEPGLPDQRIEVVAFFTDVRKAPVSL"
     gene            complement(27676..28398)
                     /locus_tag="PKL17_06025"
     CDS             complement(27676..28398)
                     /locus_tag="PKL17_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNK44318.1"
                     /translation="MQRNGGHSLAARRAEAGYSLVELMIALAVVGLISGGAVMGLRSL
                     VRSELRSQSSKLAAAIRYSYDRALTTGAFYRLHFDLDNQTYRLERSESRVLIDTKLAT
                     LSRGRGKDRDELDKEAAEEDKRALNGLPEELLPPQAPRRPKFAEYKDSTLPKVKLSRI
                     KVLDVLLPREKDPYVSGHAYLHFFPDGHTERAVIHLGTDSQDDVQYTLWVHGLTGRVE
                     VLPGRQGPPPDFDVSGSDKGVP"
     gene            complement(28413..28922)
                     /locus_tag="PKL17_06030"
     CDS             complement(28413..28922)
                     /locus_tag="PKL17_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNK44319.1"
                     /translation="MTLPTPVSASQTSVLFRLRRAARQLAQSLSHRARRDTSAPRLRR
                     VEGGFSLTEIMIALAIVSLVMGGAAVVAFGQFEKARKKETVTMIHKVKDAVLQWRMDA
                     TGEQCPGSLNDLVTAKILNKEPKDGWGRPFVMKCPGEHDTDGIDLWSFGKDGKEGTAD
                     DIKSWEDDK"
     gene            complement(28951..30255)
                     /gene="gspF"
                     /locus_tag="PKL17_06035"
     CDS             complement(28951..30255)
                     /gene="gspF"
                     /locus_tag="PKL17_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNK44320.1"
                     /translation="MPVFRWKGYDGKGKQVQGVRDSESPRTLRQALKREGIMLSDVSE
                     AQVGVGKSKKKASDSQLDTVGQGLFVGLLVALRLGFVAQAFAEYRRTAKPMEIAVLTR
                     QLGTLLRAGVQLSESLAALIEQAEDPNLKQVMSDVKVQVNEGLPLSSAMARHPGCFGE
                     LYVNMVTAGETAGNLEAVLLRLADFLESQNKLRAKVMSAMMYPIIMTVLAVGILTLLM
                     TTVVPKVTAIFADTGRALPLNTQILIFISTLVSDYLWLLLIIGIGSFVAFRRWVSTPK
                     GRLSWDTFKLRVPVLGGLERKVAVSRFAKTLSTMLASGVQLLRAMEIVKNVLGNTVLM
                     NVIEQARESIREGESIAAPLKRSGQFPAIVCHMIAVGERSGQLEQMLENVSGAYDLEV
                     ETQLERLTALLSPLMILGMGGAVFFVVTSIMLPIMQMNDFVQ"
     gene            complement(30260..>31501)
                     /locus_tag="PKL17_06040"
     CDS             complement(30260..>31501)
                     /locus_tag="PKL17_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNK44321.1"
                     /translation="VDVSDEAPIIRWVNSVIFHAVRVKASDIHFEPREKDMVVRYRID
                     NVLEEYKKAPRTFINSIVARVKIMAGLNIAEKRLPQDGRIRRKIAGKDIDMRVATVPT
                     AFGERITVRLLDKSSVLLDLADLGFGADHLETMVEMINRPHGILLVTGPTGSGKTTTL
                     YAALSKINTPDINILTVEDPVEYQLEGISQVQVNPKIDLTFAAAIRSFLRHDPDVIMV
                     GEIRDQETASMAITASLTGHLVFSTIHTNDAAGAVTRLVDMGIEPFLVTSSLMGVLAQ
                     RLVRRLCIHCRETYKPTATELSRLAIPAEDFFAGRYKVATYITKAPPPPPGHLYRARE
                     GGCNVCSQRGYQGRSAIYELLEITDAVRELSLKKADAATLKKAAIHSGMRTLRTDGAG
                     KVLAGITSIEEVMLVTAEDKE"
BASE COUNT         6178 a         9916 c         9218 g         6189 t
ORIGIN      
        1 cgactcagcc gcgctttgag ccgccggtct ttgagcagct tgccaaactg cagcagcgtt
       61 tcccgaccca ccgaaagatc gcgtcgcccg gacggacccg ccggcaagcg gctcttgata
      121 tcgacaaaac cagaaatttc ctgaagcgtt tttacataag atttcgcgat atcggcatct
      181 tcattgtgct gaatcaactg ctgaaaatac gaaatcaacg catcggtatg tagccgcaag
      241 ttgggcgaca atacgtgttc cgccaccgcc aattcagaat taggagtctc ttctgtatgc
      301 actgctgtgt tcgtcattgt tgcgtccgct aagcttttcc ggcgaggcaa ctccagtcac
      361 cggcacggct gcggaagatt gcatcagaaa cgaacaaacg tgaagagaga atcctacctg
      421 tttttcgacg gattgtggca ggcagaaggc caattcgcgg ttatatccaa ctatcgttac
      481 ccaagatgag ccgatttgcg acagagcaac gcacagatag gcacactttg gtgttggact
      541 aaatgcgatt tggtttccgt aatggaacag tctgctattt gacccagtca atgttctggt
      601 ggctgcgagg cgtcagaccc aaaatcagcc caagcgaaat cccacccagc accgcaccac
      661 ccgccgccca aaaccaccag cgcttgtaaa tcggtttgcg cgtctgtttt ttctgttggt
      721 cggcgtcgct gctaccgcct tcgcccagat caattgacag cgagacgctg cgatcgggtt
      781 cgaccagcac ttcgccctga tacggcggca gaccaggcga acgcacttcc agcggcagcc
      841 gacccgccca cgccggacgg ttgagcggag taatgcccag cttgcgctca ccgacaaaca
      901 cctcggcacg cgacgggacg cttaacactt gcaggttgcc tttgggcttc tcttgcgcct
      961 gttccaaaag atgagccagc gtcgatccaa tcgtcgcagc ggccttgctg atggtgcagc
     1021 ttgcacacag cggcgttccc gtcgcagcgc gatcacccac cgcaacgtcg atgaccgaca
     1081 agtgaagccg ccagtccgac tgctgcccca gcccgctgtg ctcggcatgc agaagcagca
     1141 cgtattccgc ttggttgcgc ttgcccagct cttcctgaca cagcagcgag tccagacagc
     1201 ttgccagttg cggggccgcc gtcatcgcca tctcgcgccg cttcaggatg tgtccactgc
     1261 catgcgccgc ttgttccgcc gcttgttcca gctgacgaat cgcttcccca tcgagcccgc
     1321 gatagtgcgg caaaaacaca atgatcgggg ccagcgatgc cgcaatgtcc tctgaatcga
     1381 cggtactgcg caaaagccag cttgatcccg agtgaatgtc cagatcctgc tgttttcgtc
     1441 tcgccgccag ttccagcacc acctggtgtt ttcccgccgg cagaagcagc ggtgccgaca
     1501 aaggcagcgt tccgaccagc cgctcatcaa cgagcaccag tgacccgcgc tcgcccaaaa
     1561 tcagcacttc gcccgcgggt ggcagaggct cggccagcac tttcggcact tcgggatgtt
     1621 catcgccaag cggtccttct ttttcccgcg cgtagcgccg aaacagatcg aacgcttcga
     1681 cgctgcgccg ctccacatac gccaagtgcc ccagcaggta caacgtatcg acatcgccgc
     1741 gcagccgata cgcttcttcg agcagctgat acgttagcgt cagcgcattg cgggccagtg
     1801 cctgctctgc tgcggccaaa agctgcgtca cccgcgcaac cgccacgcta tcggccacct
     1861 gtccgacgct gcgaaagtcg ggcttggccc aaatcggcga tggaaaaagc cacaggcagg
     1921 ccagacacag ccatgcccaa acacaggcca tacctcggcg ctgtgcagcc ttttgccccc
     1981 gcataccatc aagatcgcac aatctgtggt cctaggacag cccccgcgta gcagcgcgcc
     2041 tcggcgagtc gtcattttcc gtccgcagct tgcacagccc gaaatccgcg cagacaagag
     2101 ttgacgcagc caggtcagat ttggcatccc atgtaccaag tttggggcaa aatttagaat
     2161 ggttctaaaa ttgaccaact gacatcaaac caaacacatc cccgttgtga gccactggct
     2221 ctgacgacga aaacccaaag gagatcctcc catgagcatt caagtcgctt cgcccgcccc
     2281 ctcgttcaag acccaagccc tggttggccg cgagtttaag accgtctcgc tgtccgacta
     2341 cgctggcaag tgggtgctcc tcatgttcta cccgatggac tttacgttca tctgcccgac
     2401 ggaactcgtc ggtctgaatg acaagcacgg cgactttgcc gaccgtgact gcgtggttct
     2461 cgccgccagc accgacaccg ttcactcgca cctcggctgg gtcaaggccg atgcgcgcct
     2521 tggcaacctc aagtacccgg tcctcgccga cgtgaccaag tcgatctcgc gagactacgg
     2581 cgtgctcatc gaaaacgaag gcatcgcgct gcgtggaacc ttcctcatcg atccgaacgg
     2641 cgtgctgcgc tgggcctgtg tgaacgatgg ctcggcgggc cgcagcatcg acgagatgat
     2701 ccgcgtgctc gacgcgctgc agaccgacaa gctctgcccc tgcaactgga agaagggcga
     2761 agcgacgctg tagtagcccg attcaagggg cggcctgtgc aaaaacgccg ggccgccgac
     2821 ttggccagac cgctgtccag ggatcgaatg acgatcgccc aaggtctgcc gcgcgactcg
     2881 atgttccgtc gctgcgcgca ggcctttttt gtagcaggag tattgcgatg aagttttctc
     2941 aagaagagct ggatggtgcc cgcagccgcg ttgctgaacg tttttccgta caagctgcgc
     3001 cgacggtact ttcgcaaatc cccgctgaag tggtgcgcga tgcggcgctc aatttggcca
     3061 gggcgctcga tggagacagc gccattttgg cgcgtggcga ccgcttgctg attggacttg
     3121 gggtggcgct ggccaaaaac ggtcggcccg gtgcaggtct cgccggctgg ctggccgacg
     3181 ccgccaaagt cgcgggcaag accgatgaag atatcgaagc cgctgcggca gccgcgctcg
     3241 cctgtgcaac ctacaacggc tattacaaat ttcgttcgct tgtgtccgat agcaaaccgt
     3301 atgagtcgtt tcagccaggg ctgcgggcca gtccgtttgt ccgttcggcg ctcagtcgaa
     3361 ccctcgtcga gctgatttca ctcgccgtgt cggtcatgaa cggctgcacc tcgtgcgtaa
     3421 atggccacgt cgaaacggtc aaaggcggca acctgccaaa tcccgatgcc gcgatagatg
     3481 aagcggtgcg cataagcgct gtggtcgccg cgctcgcagc ttgggacaag gcgtaaccgc
     3541 tggcggatcc agcgcgagca aaggcaggtc ttgtcgccgg cccaaaagac agcgcaagat
     3601 agaaagtggt aacacccatg cacgacggat cgatggaacg acagcagctc ctgcactacg
     3661 aggagatttt gcgcaatgcc gaccagtttg tgacggtgca aaggcgcgca ctgctcaagt
     3721 atcttttgcg acatcgtgtc catccgacga cggcccagat cgcaagcgcc gtcggtcgta
     3781 gcggcagtgc cagcctcgcc acggtctaca acaacctggc gctgtttgca gagctggcga
     3841 tcgtccgcac cctgcgcagc ccccgcggcg aggtccactg ggacatccgc accgatcgcc
     3901 atcatcactt ggtctgttcc gactgtggcc acgtcgacga cgtatccgaa gaacaggttg
     3961 aagtaagcat ccgcgcgccc gacatcatcc atcgcgtcac ccacaccgaa gcgtggcttc
     4021 tgtggagctg ccaccgctgc aagccgacgc tgtagacccg cccctctgca caaaagcgcc
     4081 cgcctgggcc aacgcttgat ctataaatcg gcttgggcct ttttcttctc aacgtaacgg
     4141 cccgcttagc gacttagcgg agacttattc ggatgcggaa acagccacgg cacaagcgcc
     4201 gggccagccg agcctacttg cttggcttgt cggcacttgt cgcaacggaa gcatgcggtc
     4261 ttcccgatga tgccgagttt cgcggtgcgc ccccgcaggt gcagcacgtc acgattgcct
     4321 tccctggcgt ctcgacgatg tccccacggc tgccacgcga agacagcacg cccaaaaaca
     4381 ccgacaccgc gcagtattac gaactgaccc agtcgatggc ggcgctgctc aattcctatg
     4441 cgctccagct actgacggtg gcgcgggtga tggtccagct tccggcgacg acccgcatgg
     4501 gcgaaagccg aacgtggggc ccctatgaac ccggtggtgc cgacccgctg acctatcgtc
     4561 tggtcgtcaa tcggctgggc gaagcggtgt ttgcctatac gctatcggcc cgcaaccaaa
     4621 gcgccggcag cgacaacgaa tttctggtcg tcctcgatgg atcggtgacc aaaggcgacg
     4681 aatcaagcgc cgccaaaggt cgcatgaccg tccatttcga caaccgccgc aagctggtcc
     4741 ccgacagctg cgaacagggc aagatcgact tcgagtacga cagccttgga gacccagccc
     4801 tgctcgacat caccctgcaa aaagtgggca gccaaaaccc gctgggccgc aaatgtcgcc
     4861 aagatccgcc ccgcgacggt cgatttcacg tcgagcacgg cagcgatgac agcggcaatt
     4921 tcctgttcga cgttcgcaaa aacatccacg gcgacgacgc caaccgtccc ctgctggaaa
     4981 ctgtgctttt gcgcagccgc tggaacgaaa aaggccaagg ccgatccgac gggaaaatcg
     5041 ccgatggcga agtatccatc gacttgcgca acgccgggct cagcgagcgc tacgtgagct
     5101 tttcgcagtg ctttggccag ggcggcaaag cgctatacca gacggcaagc ccagaatcgc
     5161 tgcggctttt gacgagcctg ggcgatccga gcgtctgcac gcccaagacc gccgcttttc
     5221 ctgattgatg gagagatggc gcaggcagac gccgacggga ttacttgccc gtggggggcg
     5281 aagggatgtg cggttgagcg ctgcgcagca ccgcacgctg gaccgtggac accagatcga
     5341 ctccagtggc agcccgcact tgttcgttga cggcgacaac ctgggcaccc agcggcgtgc
     5401 caccacccga tccaccgctc gccccaccga gcagcgtcag cctgtcgacc tttatcgctt
     5461 tcattgtgcc ggtcagctct gaaaacaccg gcagaagctt ttgcaaaagc agcgactcgc
     5521 gcgcgctgcg accaccggct tgatacgcct gcgacagact gaccagcgcc gctgctgccg
     5581 cccgaccttc ggcgacaatc gatgcggcgt tggctttggc gtccgcttcg gctttcgact
     5641 tggtggcctg cgccgacgaa atcacatcgg cttcgagctt gcgccgcacc tgctcggccc
     5701 gcgcttccca cgcccgcagc tgcgccttgg cttccgtcag ctgcgcctgg acttggccgc
     5761 gcacttccga aatcaccgcc tcgcgcttgg tctgcgcttc cacgatgcga cgctggtttt
     5821 ctttttgcgc gacctgcgtg tcggtgtcga tcttggaaat ctcggcgttc atctggttgt
     5881 gggctttttg caccgccgcc gatgcccacg cttgcagctc ggcaatccgc gcttcttttt
     5941 tgatgcgcgc cgaacgaacc cgacccaccg acgacaggta gctgacgtca tcgctgatgt
     6001 tttggatttg caggttgtcg agcagaagcc ccagccgatg cagatccttg tcagcttcct
     6061 cgatgagctt ttgctgaaag atcatcttct gctggttgat ttcctcgggc gtaagctcgg
     6121 cgatgacacc gcgcaggttg ccttcgagcg tgtctttggc gacccgcatg atgccttcgc
     6181 gcgacgcccc caaaaagcgc tcgatcgcgt tgtgcagcag cgactcttca ctcggcagct
     6241 tgacgttggc gacggcgtgc actttcagcg gaatgccacc gcgcgaaaac gcattggtca
     6301 ccgacagctc aatcgccatg ttggtaagct gcatgcggtc tttggtttcg atgagcggaa
     6361 tccgcatcgc ccgaccaccg cgaatcacgc gatagccgac gagccgccca tccgcaagcc
     6421 gccgcgtccg acccgaaaag atcagcactt ccgacggcgg acagaccgaa atgttgttga
     6481 tgatggtaaa cagcaggaac acccctgccg cgagcaccat cgccaaaatg atggtgatgc
     6541 ctagcgcgct catcggctgc ctccttctgt gggcggcgta agcaagttct tgatgtccac
     6601 gccggtcagg cccttgagcg tcgacagaac ttcggtgaca atcgccggat aggccgccgc
     6661 cacctgcgcg agcgacgtgc catcgccggt atcgacgatc tggatttcgc gcaccgacag
     6721 gtctttgact ttgctcgcga cctgcgcgac cagcgtatca agctgcgaca gcacaaacag
     6781 ttcccgcgct tgcggaccgg cggcttgcag cgcttcggac aaaagacgca tcgcctcggc
     6841 tgcggccaga ccttgctcgc ggcgcggcgc ggcggagcct tccgacaaga gactctgcgc
     6901 tttctgatcc gcttcggcgg gcagcacgat ttccgcaaac agcttgcgct ggtttagatc
     6961 cttgcgcacc gtctgaagct cttgttcggc ttcggcgcgg gcctgttccg ctgctgcggt
     7021 ggcctcgcgt tcgaccgcct gcgcctgtcc ttcgagctga ccgacgacct gccgcagctg
     7081 gttgcgcttc tgggcgacgc caatttcggc atctttttgc gccacttcgg caagctgccg
     7141 cgccgccgct tcggactgcg cgatttcctg gttggcctgg ttttccgcgt tttccgcgtc
     7201 acgcaccgca ttggcaatct gcgctcggcc caggttgacc aggtagccgg cctcgtcttc
     7261 gacgcgctgg atcttcaagg tgtccagctg caggcccatc ttgttgaaaa tctggttcga
     7321 gacctgcaca agctggttgg caaactcgat gcggtcttgg ttgacctgct cgggcgtaag
     7381 ctgcgaaatc acgtcgcgca gcgagccttc aagcgtctgt ttggcggtct gccagatcgt
     7441 ctcccgcggg agccccaaaa agcgctcgac ggcgttatag acaaagcgcg ggtccgacga
     7501 aatcttgacg ttggcaatcg catgaatgtc gagcggaatg ccgcccgacg acagcgcttt
     7561 gggcatcgac agctcgatgg gaatcagccg cagatccatg cggtcgacgg tctgcaaaaa
     7621 cggaatccga aagtgcgtgc ccgacagcac gaccgtaaac gtggtcgctt ctcccgtcga
     7681 agtgcggtgc gacttaccgg acaccaccag cagttcattg ggacggcaga tgtacaaaaa
     7741 ctgccgcagc atccacacaa agacgagcag gccaacgaca aacgccgccg ccaacatcac
     7801 cacgccagcc agccgcgcgt cgtcggaaaa ggcctgcgga gggggttcgt cgagcgctcg
     7861 gcttgcgagt gcccaaagac ccaaggggtt ttgcgccatg tttactgtcc tttttcctgt
     7921 gcagagcgtc cggcctggat cgctccacgt tgcttcgctt gctgttcgcg ttcgtctttg
     7981 cggtcgacgc tgacctcggc ttggctgtcg cgcacatcga gaatcgtgac gagcgatcca
     8041 gccagaagcg ggccgccgtc cgacgctttg gccaaaagat ccaccgtctg ccccgccact
     8101 tcgagccgca ctttgccgca gccacccggt ggggccggaa tcagcacttc tgccgtcgaa
     8161 ccgaccatct tttcttcgac cagcgtgcca ctttcgcgcg tcacgctgag cctttgcatc
     8221 gccgcccgcg ccgcaaacgc cgtcgacacc cccaccaaaa gcgccgacat cccacagatc
     8281 ggctcgggaa ctttggcgac ggtccgcaaa agcagcccgg tcaggccacc gaacgccaaa
     8341 agatacgtcc agacctgaat cgacatcagc cacaagagcg ccgtcggcac cccggatgcc
     8401 atgccatcgc cgtcgtgcgc atgaccctga tcgtgggtgg acagaccgtg cccgtcgccg
     8461 tgtccatcgc cgtgtccgtg gccatcgcca tgccctgcgg cgtcgccatg tccatcgccg
     8521 tgcccgtcgc cgtgtcccgc tgcggcatcc agatgaccgc cgtgcgcctg gcccgcctgc
     8581 gcgtcgccgt gcgcctggcc ctggtcgtct ccgtcggact gattgtcatg ctggccgccg
     8641 ccgaaaagcg acaccagcac gagcagcccc ccggccaccg cagcaaacac atacaggtac
     8701 agcacgcaaa ggattgtcgg cccgacctgc accccctgtc aaacccacgc cgcacccaaa
     8761 tccccccgca cccgaatcca ccccacccca taagtcccca agacctctct tccccctgtg
     8821 cgcaaagcat gctgtcatgg cgccagggtc gaaatacctc agcacgatga tcgaacgcat
     8881 ctacctcgac aacatccgga ctttcgtcaa cttcgagtgg aagccagcca agctcgcgct
     8941 catcctgggc ggaaacgggt cgggcaagac agctcttttg gcaacactgc gggcgattcg
     9001 caggtttgtc ggtggacagt acagcagcga ggaagccttc ccagctacca gcctgacgcg
     9061 atgggaggac aagcgtacag accagacggt cgagatcgat ctgcgggtgg gcacggattt
     9121 gtttggctat cgcttggtca tcgcgcaaaa ccccaaagag cctggcgcaa atcgcgtaaa
     9181 gcacgagagc ctgcgcaaaa atggcgagct gtgcatccag cgagaagaaa acaagctcga
     9241 gctggcctat gaagtcggct ggccgcagtt gtctatcaag ccgacgcgct cagcgatgga
     9301 catcggtgac aatcgaacgg acgccccatc aagtgtcttt cagtcggcgg tctggcacct
     9361 gtggttctta caacccgatg cccgtcagat gacagcacgc cttgcagaca gtccacaggg
     9421 agagtcgtcc gcctggcttc gccacaactt cagcaacttt gcagactggt atctgcgcat
     9481 ccttcagacg aggcccaagt cggtcttttc cgcgctgcgg acgctcgaag agatcatccc
     9541 cggattgcaa gaactgcatg ccgattcggg gtggctgtac gcccgctttg agtcagaggg
     9601 gaagacggca agcttccgct ttgacgagct atcggatggg caacgagcac tgattgcgct
     9661 tcacctcttg cgccacgtcg cggcaacacc cggaagcgtg ctggcgctgg acgaaccgga
     9721 caactatgtg gcgctggcgg aaattcagcc ctggcttttg gatgtggagc gggccgcgct
     9781 aaagtccgga gggccgcaag tgtggcttat cagccatcac ccggaagtca tcaacctgct
     9841 ggcacccgag tatggggttc ggtttttccg tgacggcatg ggtccaaccc gcacggcgcc
     9901 atttcagtct gcccagcagc tgagtgcatc cgagacggtg gctcgcggat gggacaatga
     9961 gccgtagcgt cagcatcctc cttctgtgtg aagacaagca ggcgcgggtc tttgtcgaat
    10021 cagtgctcga acagattgat ccgagcctct tgcgacggct ccgcgtagta ccatttcccg
    10081 tcacaggtcg caccggaacg gcacaaggtc ttgtggacgg ttacaaagtc tatccatgcg
    10141 gcagcatgca tatacgcgaa aactatccgc agctcgtcag ccagcgaagg cagaatgcgg
    10201 gtcgcttccg cgcgatgggc tgccgcctga ttgttcaaat cgacgtggac aactcggaaa
    10261 aaagcccacg ctcagtcgcc gaccgccggg ccgagcttgt gcgtgcctgc gaagcgcaaa
    10321 agatcacgcc gcccgctgca aacgagccag tggcgcacct cattccgcgt cgcaatatcg
    10381 agacgtggat tgtgtttggc aactcgggac agcctgtcga tgaagggacc gagtatcccc
    10441 acctgacagg acacgagtct gactgcgagc cggctgcgcg gaggttcgcg gacgaatgca
    10501 agacgaacca gccgagcgcc ttggctccgc cgtcttggac cttcgggctt tcggagctgc
    10561 gcaaagtgct ctgacgacag tcttggctgc gcgcaaatcg gcggcgggct cgtctgccgc
    10621 tccttttggc ttcccgcaat tggcggcggc ctcgtcttcc gctcatttcg gattcccaaa
    10681 atgggcggca ccccagcctg ccgctcgttt ttgtttgcag aaatgggcgg catcccagcc
    10741 tgccgctcgt ttttgcgcgc ggaaatgggc ggcaccttgg cctgccgctg attttttttc
    10801 cgacgacagc tctgcggttt gggtggcgga acaaaaaatc tgtcctaacc tcctttcgtt
    10861 tcacttcccg acagaggagc acctgatgcc gcagattcgc gatgaacgga ccggaaccga
    10921 tgaactgatt cgagacggca gctaccacta tgcggcgctg ctggccgacc cggacgcggc
    10981 ccacttggcg ccgcttgtga aaaagcgact ggacgcactg tccgccgcgc aggctgacat
    11041 gcacaaagcc gagctggggc ggattgaagt gcaggcgctg gccgagcgcg ccgaatacca
    11101 gcatgaccgt cggcagagcg tgctggaact ggaagtgctg gcggcggtcg gacggagccg
    11161 caaaaacccc gcctaccaag aggtctatcc gcacggcctg tcgggacttc tgtcgctgtc
    11221 gggggaagag caagaacatg cgaccgtcgg gcttttgcaa aagctgaagc aacatcaccc
    11281 ggatctgtgg aagacgcaca gcaaagagct gaccaagctg gcgcaggatg cgacggcagc
    11341 ggaaaaagca ctgaagaagg cgcaggtgga agaaagccgg gcgttcctcg acgaacaggc
    11401 ggcgcgggcc gagcttgtgc ggcagcttcg caaaaacgaa ggcgcgctca tctcgctgta
    11461 ccccggcgag cgcggcgcga ttcggctgta ctaccggcag ccaaagaaac gaaccagccc
    11521 ggcggcatcc cccgcgccac atcccgccta accactcggt gtccggcttt gcgtgtcatc
    11581 ccagcagagg tcgcggtacg ctgtaccgat gcgacttggg tctgcactgg ctttcggcct
    11641 gcttccgatt ctggcttgcg gcacggggtg tcacaaaaag ggcgcaaagc aggcgtcgct
    11701 tgtgctgtac accgggtacg gaagctcggc gcctggaatg ctgtacggac gggtgcaaaa
    11761 aggcccgccc gacccgacgc ccgaacccgg acaaagcaag atccagcgca tccaagagac
    11821 gctgcaagcg ctggacgcca gtgcgctgcc gcacgcggcg gtggacatca aagtcggcga
    11881 ccacttgcta tcggcaacaa gcgacgaccg cggctatctg gaagtcacgc tgccggccaa
    11941 gcttgcgccg cccgcggtgc cggtcgaaat tcacttgcgc acgccgggat atgacgcgcc
    12001 gccacttgcc ggatcgatcc cggtctacga cggaacggcg ggtctggcgg tggtatcgga
    12061 tgtcgatgac acggtgctcg attcgcagat caccgacaaa tccaagatgg tcaaaaacgc
    12121 gctgctgcgc agtacgtggg aacttgtgac gtttcccgac gcgccgcagg tgctcagcca
    12181 gtttggcaaa gacaagccgg tgttctacct gtcgggcagt ccgtggggct ttcaaccacg
    12241 aatcgccagc ttttttgcgc gggtgggact gccgatgggg acgatcatcc tcaagcgctt
    12301 ttcatcggaa ccactgctgg atcagatggg cttcaagtat ccgcaccttc tggacatcgc
    12361 gaaggcgctg ccggaaaaaa aactgattct gtttggtgac tcgggcgaaa aagaccccga
    12421 aatctatgcg cgactgcgca gcgaacagcc aagccgcgtc gagcaaatct acatccacct
    12481 ggtcaccaaa gaagcccccg attccccgcg cttcaccggc atgaaagtct tccaacactg
    12541 gtccgagctc aggccctaac gaaacatccg ctgtgggggc accttccggc gcgctttttt
    12601 cgcggtgcgc ggacgtgggg aaggtttaga agaagatggg gtttgagacg gcgaaggggc
    12661 ggcggccagg gtgaacgggg gtgaggtcta acccgcccgc cgaggcgtgg aacaccaccc
    12721 agtgctggct gcgtccgggc ttgcccatca cattgacggt cgtttcatag cggtgccctt
    12781 gcggcgtcac cgtctcgcgc agctcctcgg ttgcaatcgt ttcaccatcg acgatcgttt
    12841 ccagccgctt ggcggtaagc cagcttgggc tctgcaccac gacctggaac tgcactgggc
    12901 gtcccgccga acgaatctgc tcaccggggc cttcgccgtc tggtcccttc accgtcatgt
    12961 acagcccgcc cgacaccacc gctttgccgg cccgcagcac gtcccgcacg gtttccgccg
    13021 aaagccgtgt cggatcgtca tggccaaagc gcaggcacgt ccgcgggtag ccgaccggac
    13081 tggtgcgcag accgtggctg tccgagctgc ccacggccca gacttttttg ccggcgttta
    13141 gcagtgcgaa ccagtcctgc acaatcgcgt tgcggttgga ttcaaagtcg ctgtcgttga
    13201 agacttcgag cgcgccgtac tcatcgctgt acagatccgg gtcgccgctc accgtcgcgc
    13261 gatcaaagct gacggacgaa aagtagccgc caaagctgtc cgagcgcggg tgattcacaa
    13321 tcagcaccgg cttttctgga agctgcgcaa tggtgtggaa cacctcgggc ggttttttgc
    13381 cgtaccactg cacggcgcca cggttcggtt cgtccggacg cggcgtaatc ggaatgacgc
    13441 cgaagtggcc ccacgcaaac gtcgtaaatt cttccgacgg catcccaaac gcccagcggg
    13501 taagtcccag gcgacggaca atcggctgaa agtcgatgat gtactcgtgc tcgctcgaaa
    13561 tcgggatttc gagcccgtct gctatcgcgc ctttgacctt gtattcgacg tcatcggacg
    13621 agtcggcgga aaagtacgag tggatgtgga agtcggcgca catcacgcct tggctgtcga
    13681 cggtgtgcag aagctcggcg cggacttcgg tggtcttgcc tgattcgacc gtgacagtct
    13741 gatcgaacaa gtcccactcg gggccgcggc ttatgatgac gcgctgcttg ccggcaggaa
    13801 tcgccagctt ggtgctgccg ctcacggcaa actcttgcac gctgcgcccg ccggcttcgt
    13861 cacggacccc ataggaatcg ggaaacgacg gcggacccgc ctgcggaatc acctgcaccc
    13921 gcaccggcag cgcgacacca ctgccttttt ccaccgccgt gaccgacagc gttccggtcg
    13981 cttccatctt gaggttggcg gtgttttcag tcggtgcgac gatcttcttc accgatggcg
    14041 gctgtccgta ctgggtcgcc gatagctcga ccggttcggc gggcagatgc agggtgtatt
    14101 cgccgcgcgc gtttgtctgg gttcgcgcaa tgtaaccgcc accgcccgat cgcagcgcat
    14161 gcacataggc accggcctgc ggcgcgccgc ttgtatcggt gacggtgccc gtgacggcgc
    14221 gataggcggg ctgacccagc acccgacgca ccgcttcgga aagtccgtcg gaatgcggac
    14281 cgccgacgat gaactcgacg taatcggtgc tgagcggact gcatgccttc gcggtcaaac
    14341 cagcgccgcg accgatgata gaacccgagc gaccgagcag tggcgtatag ctgcccgaca
    14401 gaaagtgcat cgcgaacgac gaatcgccag agtcaaaccc gagccactgc tgatcgccgt
    14461 tcaagtcgcc atagccggct ttttcggtaa aaagctggcc gcggctcgac tgaaacagca
    14521 caaagagctg atagcggtac agcggcagtt cttcgtcgcg ggtgttcagg atgtgcagcc
    14581 gcagcttcac gctttcggca cccggcaaaa ggatgtagtc gatcgccgcg ggcaggtaga
    14641 agttgtcggc gaacaccgat cccagaaatt ccagaaacgg caggtcggtc agcacgccgg
    14701 atgtacgaat cacggcggca ttgccatccg atccgtcatt tatcacgctg acggtttcgg
    14761 cttttagcac ctgacgaccg agcgccatga tggtctcgcc gtacagtgag ttgccgctcg
    14821 gtttgccatc tggaccgacg gtatcgagcg acaggatttc gcctccgaac ggctgatagc
    14881 catcggaaac gcccggcgct tcgatgtaga ccgcgatctt gtcgttgatc agcagatagt
    14941 cgccggtgcg caccggatgc cgcgcggtgt ccgcctggcg aatccaggcc ggatcgctga
    15001 tgcgtgacgc ccgcgcttgt ttggccgcct tggccccgag tgggtcgcga tggccttgcg
    15061 cgtcaccagc gggcagacgg ctcttttcgg acagaccctg tgccgcacac gggcccggcg
    15121 gtgcatcgtc tggcacaggc gcgtcgttgt gcgagcagga caaaagcgcc accgaaaaga
    15181 gcggcagcag cggacgtagc cacggtcggg aagcaaactt tgccatcgcg agcacctccg
    15241 aaaggaggca ctagaccaaa tggcaagcga tgcagcaagg ggaggatttt gcccccgccc
    15301 aagatgcgcg cacggaccgg gcgatccgcg acgaacgggc cagggtgaag cgggcgatta
    15361 cgggaagtag acgagctgaa gtggcgagcc ggcgctgttg attggcgtcg aagctcccgt
    15421 cggtgcaaac cagccgcagg cggcggcgct gccgttgcca gtcaagttga tgacccgcac
    15481 ctgaccatcc ggcgtcggcg tcgccatgcc ggtcgcggtg gttccgccca gcacaagcgc
    15541 gcccgtggcc acggcgaact tggtcccggt caggtcgatg ttggcggtgc cagcggtggt
    15601 ggtgccgttg caagccatcg ccgtcgcata cggcatcgcc gtgatgttgg tggtgccttt
    15661 ggccgcagcc cccgtcgaag ccgcgaaacg cctgtcattg gtgtcgatct tcagcgttga
    15721 aggatcgatg agcagccggg tgtagttggt tttcaccgtc gtgcccgtca cgccaccacc
    15781 ggtcggaatc tgcgaaaagt tctgggtcgc agcgacgttg gtcagcggca gataggaaag
    15841 cgctggcgtc accgacatca cgcagtaggc atcccacgac ttggtcttgt caccgccgac
    15901 aaacagcttg cgcgcgccgt ccgtcagcgt tgggttgtcg gcgcggagct gctcacagct
    15961 tgccggattg ccgaagtttg ccgaaatctg aacatctgac gtaagcgtga tggtgcaggt
    16021 gcgcgccgat cccgtgcagc cgccggtcca gccggtgaaa tacgacgtgg tgtcgggcgc
    16081 cgcggtcagc gtcagcatcg tgcccttgtc aaagccgccc gagcaggtgc cgcccgaaca
    16141 ctggatgcca aacggcatgc tggtgacaag tccaccgccc gcgccgccga tggtcagatc
    16201 gacattggcg cggtttgtct tgctcttgaa agtcgcaccg acgtttttgc ccttgtccaa
    16261 agacagcgtg caggtcggag agctgccggt gcagtcgccg tcccagccga caaagtcggt
    16321 gctgtcgctc agattcaccg tcagcgtgat gctctggccg accgcgaacg tgctggcgca
    16381 ggtggtgcca cagtcgatgc cggcaggctg cgaaaccacc gtgccacccg aaaagtcgcc
    16441 cttgagctgg aggttgagcg ccgccaccgc agtgccatcg ctcgattgat acccaccaca
    16501 gcccacagcg gaaagcaaaa gtcccaaagg caacaccaac gccgatcgac acagaagctt
    16561 cttgtttttc atatgctgtt tctcctcgcg tattgagacg aaagacgctc gatgcgaaaa
    16621 gcactggttg gagccagggc gcgcacgaag gtcagagcaa gctcgtggtg attttatcat
    16681 cgcgccgtgc ctgctttcgc aagtttcgtg gccgggctct ggcgtcacac gttcttggtc
    16741 agattgggaa gcgctgcgcc gatttgtcgc gcaccgaaca gcggcccaag cggcggcatt
    16801 agcagtcgga acggaagacc cagacgccgc ggtgcagatc gcgggtttcg ccaagccccg
    16861 gcagatccac cgagcgctcg acttcttctt tgagctgcgg aaatgcgtag acccagcgac
    16921 gaattttggc gcgcggaatg ccgcgttcga ccaatatctg ttccattgcg cgggcccgcc
    16981 gatgcgcttc ctgttcccca ctgtcgggtg acgacacgat caaaaactgg gtgttgcgca
    17041 gaagcgccga gcgcaaaagc ttgtgcagcc gctcggcccg aaacggaatc acctggcttg
    17101 cgccgtcccg gaaataaaag acctcgtgac gcagatgcga catcagcttg agaaaccggc
    17161 ccttgtgttc gggatctgcg gcaatcaccg ctgggcgaat ctgtttggtg gtgcaggttt
    17221 cggtctgcgc ttcgcactcg cgctgcacgt cttcgataag ctggctgagc tccggcgtgc
    17281 acatcgccat gccgattccg tcgccgattt cgagccgcag ccgctgaatc tgttcgctct
    17341 gcgcttccaa aagctgctgg agcgcatcga cccggtccgc aagcgacggc ccgcagccgc
    17401 caaatcccaa cccgataagc ggacccagca gccacagacg aaaagcgcgc attggcaacg
    17461 accacgacat cagcaaggag tggctactat cacaatttgc aatccggacc gtagactttg
    17521 cagcctttgc ctttggaccc gacctgggct accttgccgc ccatgttgcg agtgtcgcgt
    17581 gtccgtaaag cccgtcggaa agggcctgtc gtggccgcgg cgatgtctgc tgtcacgctg
    17641 cttttggggg aatcggcggc gcacgcggat ctcggttcgc tgggcagtac gacgattggg
    17701 cttgcgctgt cggcgggatt tcagaccgat ccgcggacct gccacgtcgc cggtgacaca
    17761 cgcaacgcgc cgggaactgg ctgcgcaatg ctggtcgcgg gaattgaagg cgactttttg
    17821 tggcgcggtc gaattggcat gcttttgggg ctttcatcga cggcgggaca ggccaatgtg
    17881 ccgacgacaa gcatgcccgg cggcgatgcc ccacccgcgt ttcccgatcg ggtctcggtg
    17941 gtggctggac tcgatgtgcg tccgctcggt ttcttcatca atcgcgagga tcgatcgtac
    18001 aaagcgcggc tgtttcacgg tctgcgaatg gggatcggtc cgtcgctgga aatcgtccgc
    18061 accgcgctcg attcttcgat tgctgccagc agccgcagtg gcgcagttgc gagcagcttg
    18121 attggcatgc atgcgctgct ggatggtgaa attccgctcg ttgccagcgc cgcaaattcg
    18181 ctgtcgctgc gggtgtcggt gcgacttctg tatgtgccca agatcagcct aaatgacggc
    18241 acggtgctgt cgcagccgtt cgatccaacg tcgagcagtg agccgcaagg ctacggtttg
    18301 cgaacccagc tgctgtttgg cctggcgtac tacttgtagg cgcgtgcttg tcgcgtttgg
    18361 gccgcttgtg ctaggttgcg ccatcttcgt ttgggaaggg ttttctcgac gaggccaacc
    18421 gaaaaaagag ccgccatgga actccgcact ttcagctttc tcgacatcct tcagccgcag
    18481 ttgacgggct ttcttcaaac ggtggcccag ggctttctgc cgctggaacg gcaagcgtcg
    18541 ctgatcgtcg agatcgcgcc gggcattgcc gtaaatcagc tcaccgacgt ggcgctcaag
    18601 caaacgcggg tcattccggg catgcagatc gtcgagcgag cctacggact tttggaactg
    18661 cacagcttcg atcaaggcga agtccgatcc gccggagcag cgattttgtc ggcgatgggc
    18721 aaagacgaaa gcgcgcggct caagccgaaa gtgctgtcgg aacagacgat caccggcatc
    18781 gagggctatc actcgcagct catcaatcgt ttccgtcatg gcgacatgat tggcgaaaag
    18841 cagacgctgt acacgctgga gcttcagccc gccgggtacg ccgcgctctg cgccaatgaa
    18901 gcggaaaaag ccgcgccggt gcatctgctg gaagtcgtga cgtttggtgc ggtcggtcgg
    18961 ctgtggctcg gtggatatga agccgagatc gaggccgctc gcgaagccat tcacgccgct
    19021 ctcgcgggac tttccggacg gtaagccatg cagccggggg ccgcggtgga aaacaagacg
    19081 acgacacggc ggacacgcaa aagcgagacc gcgcggattg ccgacatcct cgatgagctg
    19141 gaccggctgt atcccgatgc gcactgcgag cttgtgtaca aaagtccgtt cgagctgctc
    19201 atcgcggtga ttttgtcggc gcagtgcaca gacgatcgcg tcaacatgat cacaccgacg
    19261 ctgtttgcgc gatttcccga tccggcggcg ctggcccagg ccgatctcga agagctcgaa
    19321 aagatcatct attcgacggg cttttatcgg aacaaagcga agaacatcaa agcctgcgcc
    19381 aaaaagctgg tcgaaaagtt tggcggcaag gttccgcagt ccatgccgga tttgctgtcg
    19441 ctgccgggcg tcgcgcgcaa gaccgcgaac gtggtgctgg gaacggcgtt tgggattccg
    19501 agcggtgtcg tcgtcgatac ccacatcgga cggctcagcc agcgactcgg gatgaccaaa
    19561 caggacgatc cagaaaaaat cgagctggat ctgcaaaagc tgtggcccaa agaccgctgg
    19621 atcatgtcag gccaccgtct gatctggcac ggtcgtcgcg tctgtttcgc ccgcaagccg
    19681 agctgcgcaa cctgtacgct gcgaccggtc tgcccagcca aagaagccaa agagctgccg
    19741 tagcgagcta ctgtccgctc ggtgctcagc gaacggcgtt cttccgctct gcgatgtggt
    19801 cgcagactcg cgtgtacaac gtgctcgctt cgcgcagaca cgtttgactc aactctttgc
    19861 tgacgacctc tcctgtgtag tcgatgtcgt tgcgctgctt tcgcagcttg tcgagcaaaa
    19921 tcacgttcgg atactcgagt ccaatcgtcc gcgcaagtga ctgcaccatc gtctgatggt
    19981 gccccggctt gctggtaagc gttcgatatc cgttcgcttg cagcgcggca ttggctgtct
    20041 gcatgatcgc tttgtacgca agatcgaatc gtccctcggg acttagccca tcaagctgcg
    20101 catctcgcaa gctcagtgct gcggcatcga gaagacgacg cactgcggct tcgtccattt
    20161 caattttttc gagagacaga ccgaccaaat tatccaaggt catctgggcc ccctatcacg
    20221 aagagcttgg gtttggccag aacgtcacaa atgaaggcac cgcccttatg caaaagcgct
    20281 tgccacgccg gggccgaaaa caccttgggg ttgatttcgc gcccaatcgt agcctgcgcg
    20341 ggaaaaaggc attcaacagc ctcgccgaac tcgacgtcgc caatgatcag gatgtcgacg
    20401 tcactctgca tggtggcttt tcccgtcgcc atcgaaccaa aaacgaaagc cgcacgaatt
    20461 ctcgtcgtca gcggcaaaag agccgccgcg agcacatcag cgagtcccga ggtcttgcgc
    20521 aggatgctag ccagttcctc aaagaccagg gcgccacgat tcgcggcata ccgaacctgg
    20581 ttgccttgcc gctcccgaat caacagtcca gcatcggcga gtttgctcag ttcacgggtc
    20641 agggaaccgg ttgaacttcc ggtcaagcga gcaatctcac gcacatgaaa gctctggctc
    20701 ggatgtaaca gcaggaggcc aagcactcga cgacggtagc cagggaaaag tacggaagcg
    20761 agggacatgt ggggaaaacg ccaactgatc taaaaagagc accatttggt gccttttcgg
    20821 aaacactgtc aactgctacc ggattattgt ggggcgggaa cggggctggc ttcgggcttt
    20881 ggggctgatt cgggggacgg ggaatggggc tgttgcgaag gagccgccgc ttcgcttggg
    20941 gtcggctgat tgggcggcgg cacatgcaga gttcccggcg gagggccggc gctcgccagc
    21001 ggattgggcg gcggcggcgg tggactggga atcgcctgct ggatcggtgg cggattttgt
    21061 ggcggcggcg gcaccaccac tgtgtcactg ctcggtggtg gcgggctggg aatcgcttgc
    21121 ggctgcggcg ccgcttggct tggcatcatg ggtggcggcg acggcgacgg cgcaggcggt
    21181 gtaccgggtg aaaaggaagg ccgcgctgcc ggcgacacct gtccgagcgt cccggcgcgc
    21241 agcaccactc cgtccggggg atctttggcc gccttggcct ttggattggc gagcgaaccc
    21301 gtgatggcaa aaccaatggc cccatcggcc cgcttgccct gcgactggct gttgaccacc
    21361 agttccatcg tcggttcgcg cgcggtaagt tgcggcgacg gcttgaagcg cacatacagg
    21421 cgcgcatcgg aaagctggat cggatcacgc agttccagat agccatcgac ccacagctcg
    21481 acgtcgggac ttttggcgtg aacgtcgaca atgtttgcac gtccgcgctc gatggtgact
    21541 ttgccaatga cgtcgcccag gcgcagcttg ggaaacgtga tgccttgcga caaaaacgca
    21601 tcgcccggta cgaccagctt ggctttgccg tcgccgatcg tcgcttgccc gagcttgatt
    21661 tccagatcac cgaccgcttt tgcgacatcc agccgaggcg gcggcggatt gagcagcggc
    21721 gccgcaccag cccgagcagg agtcgccggt ttttggttcg gcgccttgaa gtgcagagac
    21781 agcgccagcg tcccgagcat cggcagaggc aaaagccccg acagcgccga cagccgcagc
    21841 agcgtcacat cgtgaaggtt ggcaaccacc tggacgccgt catcaccttg gctgccttcg
    21901 gcggtcagct cgccaccaaa cgcttcgacg gacagcgatc cgctctttgt cccaaacgcc
    21961 gcatcgagcg gcttcacccg cgcatccagc ctatcaatca caatcggtcg caggtttttc
    22021 gccgctgccg catcgctgcc gggaactttg cgtggcttca gtcgcacgtc cgtcgctgac
    22081 acaccgggcc gcagcacatg cagatccagt tccccgatgg cgacatccat gccggtgcca
    22141 atgccaaagc cgcccgtcgc ggaaatcggc ggaccttgat cttgggacag ctcacgctcg
    22201 atgcggtctt tgattttgtc gatgggaagc gacagcgaca aagacacaaa aaacgtcagc
    22261 gtgccgaaca cggcgtaaag cagcaccgtg acgacgcggt tggtcttccg cagccgtcgc
    22321 aaaagacgaa tcagttgccg gcgcattacg gctccttttc cgcaggcgag tccgtcttgc
    22381 catcgccctt tttgtctttg gccgcggtct tcggcgcatg ttcatacgtc gagacggtca
    22441 tgtcgacctc gaattcctgg tgtttgtcgt cgcgagagcg gacgttaagc tgcgtcacca
    22501 gcaccaagtt cgatggatac gtctcgatct tgtgcatgaa cttgagcagc gactcgaggc
    22561 cgactttgct gatccgcaga tcgaccgact gctgcatgta ctttttgccc atcggctcgg
    22621 gcgtgcgcgg gttggtttcg cgaatttcga tgctgttttc tttcgccaga ccttccaaaa
    22681 aacccgaaag ctgaagcggc gtcgtgccaa tccggcgatc cagttcctgg gcccgcgccc
    22741 gcgcttgcag atacggtccg cgctttttgg caatgtcgcg cagggcccgc tgcatcgcat
    22801 cggtctgttc ttccagggtg tcgatgcggt cgtacagata caccgccccg ccgagcatgc
    22861 cgagtccgag cacggtaata accagcgtca tgagcagtgc acgctctcgc ggagccaatc
    22921 cgtcgagaaa ttcgcgcaca cgggcaaatc cgggcacttt gccgatgata gtgggctcgc
    22981 tgctgctcat cgtcgagact ccatcacaaa ctggcgaacg agggagagac catcggctac
    23041 ggacatgtcg tctcgatctc cagtgaaaac tgctttactt cgaccggttt tccgtcgggg
    23101 cctgtgtttt ttaacgtcaa cactttgccc ttttggactt tgcggaagca gggaattttg
    23161 cccaagctgg tcgccagatc atcgacatac tgcgctgatg cacaggtcgc tttgatgtcg
    23221 gtctttttgg gccgaatgtg cagctcgatg acatcgagct tcgcgtcgcc tgcggcattg
    23281 ctcgacggag ccgccgccga aatgtcttcg accaaatcga gcgccgaaac ctgcggaatg
    23341 ctctgtccct gcgctttgtc ttggctgagc gctgccgcga gctctgccga cactttgtgg
    23401 ccgtcggttt ccggtttttc aaacagcgac gtcgattcac tgatgagctg aagccgcagc
    23461 cggtcggatt ccttttccag ctgcttgagc gacgcatacg cccacagccc cacgcacgcg
    23521 accaacgaca ccgcaaaacc gacgagcagc ggcgcttttt cgcgcagcat cgcgtagtcc
    23581 gtgcggaatg ccagctcgcc tttgcggaag ttgaagttcg gtccacccag cgcggcgcca
    23641 aggccaagcc ccaccgcagc agttcccgtc ggccagcgat gcacaagcgc cgtcgatgac
    23701 acatacgtcc gcgatgcatt cgcttcgcca aacgtcatcg attctccggc tggcatttca
    23761 cgcacgcccg gacccagcca cgttgcacct tccggcggca aaagcggctg caccggaacc
    23821 cccagttcct cggccagcag gtcttgcaca ccgtgaagct gcgccgtccc gccggtcaga
    23881 aacagcgctt tgggctcgct gccaaaacgg ctcacatacg ttcccagcgt ctgcttgaga
    23941 tcacgcaaga gcggtcggag cgaatcacga atcactttgg ccacccgcgg attggaaaaa
    24001 tccgacagac cgttgtcgtg tttgagcttt tccgccgcga taagacccat gccgatgtgc
    24061 ttggcgatgg cttcggtgat ctgctggcca cctcgcggaa tcgtgcgcgc aaactgcgtc
    24121 agcacctgac cgggtctttt gggatgcgcc gccagtgcca gcacatgggt catgcgatgc
    24181 ccaagatcca ccacccacag cggcaggtct ttttgcggca tcgagccctg tttgctttga
    24241 atccacagcg ccgccgtcgc caaagccgtt gaaccacaga cgcgcggatc gatcccgagc
    24301 ggtccgagcg aaccaagccg cagctcgacc gattgttgtt tggcacaagc cgcaagccac
    24361 agcgacgccc ccgaaccagc gggaacatcg gcaggcagct gatcgacaac atgcccgtcg
    24421 aggtcttcca tcagctgacc ggccagctcg aacggcagcg tctgcgcgac ctttttggcg
    24481 tccgaaagcg gcatctcgat aagacgcagc gtcacgtctt cggaaattcc gacggcacag
    24541 ctctcggcgc gtatcttttg tcgccccaga atcgtcagca gcgcccgcat ctggcggtcg
    24601 agcagcgaat cggtcgtcag atcaatggct ggcgatgcat cggctgccgc catttcgctg
    24661 tttagcgacg actcgtgctt gcgatccacc gctttcggag gcagcggcgt gccttcaccg
    24721 ggctcgggaa cgcgctcttc ctcgatgctg gccagatgaa cggttcggaa gccctgctcg
    24781 atgcgggcca ccttcaccga ataactgcca aggtctatgc ctaatatcgt cgacatttca
    24841 aacttccgtc gctaatcgag tcggtaatac agccaggtgc cgtttttgaa acagcttggg
    24901 tggtttacgc aaaccgggtt catgttcatg ctctggctgt cccagacgcc gcgaatgtgc
    24961 acctgggtgt ttttctgagc ggaacgctgc acgctgccgg tggcatcgat gcggaaaaaa
    25021 cgccgcggac tcttgcctgc gctcgacccg ccttgagccc cagccgccgc ggtgggacga
    25081 tcacgcagaa aacgcgccag catccctggg cacagcgcta tcggcttcag cggtgtcgtg
    25141 agcgtcggcg gcgatgacat gcccgtcatc gatgacaacg actcagccat ctgcggcagc
    25201 ttttcaatcg cgctcgaaca gatcacattc gacagcgttt cgatggcatc gctggccggt
    25261 gggggagtcg cggtcttggt cgtcgtactg gtcgtcttgt tcgtcgtcgt ttgcggcgtt
    25321 gcgttcggac agcgcgggtc gggcttgcac gaacccagct tgtttagctc gcccgacatc
    25381 gacttgagca tcggcagacc cgacttgagc atccccgcaa gctgctgcgc gacaaacgac
    25441 gtgttgggat caaagaccgc ggtgcgatcc actgccgacg ctgcaatcat cgccgccaag
    25501 agcggccacg ccgtcggttc catcgccgat gccagcacct tgcaatcctg cgttccgtac
    25561 acggtgaaaa agtcgccaaa gtacgcccac aagtcttcgg aaacaccgcg gacttggatc
    25621 atttcctcga tggtgtcgaa gtactgattg tgcggttcgt accggtcgcg acctttgtca
    25681 taggtttctt cacttgcact gcccgcaccg acgggagaat aacgtgacac atcgacatcg
    25741 gtccagtcga tgatgttggt cggaacgtct tcgcggttta tcaccacgcc atcgcggtcg
    25801 ggaacctgaa acagccggtc aaagcgcggc ggacgcacca gcgaatccag cagcaagtac
    25861 aagctgcgct gcgcttcttt gtttgggtta tagccaccgc cgcagttgat ggggaacttg
    25921 ccttcctcac ttgtgatcgc aagatcaaac gaagtgcctt tgggagtccc catcccttcg
    25981 acgctgccga ggtcgattcc caccatcgcg ccgagcccat cacgggcttc tttgtcaccc
    26041 ccaaacgctt gcgccaaaaa tcccgcgaag tcggtgatca caatcgcatc gcgaaactcc
    26101 gccggcagcg actggagcat gttctgcatt ccgccttgca ccgccacgag cagcttcgcc
    26161 agctcgattc ccgatgtcgc cagatattcc gcgcgcagca tgtcacggct gttggcggcg
    26221 gcttccattt cgacttgggc gttatacgcc aggtctgccg tcgtcgcccc aagcagtgcc
    26281 aagctcacga gcacagtcag gatcgccatg ccacgctgcc gccgtttccg atccggtttt
    26341 ccggtcggaa cgctcgctac atcaaggctg ccgcagtctt ttcgatccat gttcgtactt
    26401 ccgtaaaaaa cgacctagtt gggtttggta tccagcttct gaaacatcgc aattcggact
    26461 tcgctctgca gcggcagttc ttcgccgcgc tcgtcgtgaa tcaccagttt cacgcgcacc
    26521 cgactgggca gtttgttggg cattccgtcc gcctgggtcg tgcgccaggt atcaagccac
    26581 tccttgcgaa ccggttcgta gtacgaaagc tcgagtcgca cgacatcgtc ggcgatgatc
    26641 tcggcttcac cgggaatgtt ggcgttgtcg atgcggtcgg cttgcagacg acgtgtctct
    26701 ttgcgccaca ggttgaactt gccgccgtcg cttttgtctc gcaggccgaa gtacgccacc
    26761 gctgcggtat cgccttcctt ggattcggca tacagccgct ggtggccaaa ataactaaag
    26821 cgcagctcgt cgatgtcggg ccgattgaca ccatcgaaat aggtgcgcgg agtctgctct
    26881 gtaccgacga cattgttgtc ggacagatag gccatctgca catcgcgcaa catccgatcc
    26941 atcgccagcc gtgcgacgcg atagcgcgcc attttcgatt cgacggcttt tttggtccgg
    27001 aatgtgccct ggctcgtcgc ccacaaaagc acggtgatcg acgacagaat gcctatcgac
    27061 accatcactt cgatgagcgt aaagccagcc tgccgccgtt tcatagcgac accggagcct
    27121 ttctgacatc ggtaaaaaac gccacgactt cgattcgctg atcgggcagg ccgggctcgt
    27181 cccactcgat gcggaccgtg acccggcgga tgccttgttc cagcatgtct ttgaccggac
    27241 ccaaaaagcc gcccatgctg ccccccaggt ttcccgatga agaagacgac gatgcggacg
    27301 ccgtcttgtt tttgctggcc gccgactgct tgtcttgcaa aagcttggtc gctgccgact
    27361 gcatctgatc cgtcgctggc aagcggattt tttcgatgat ggtgacgtag cgaaactgtt
    27421 tgaacgccgg gtcctggaaa tcgccgcggt cttcctttac acccgcatcg tccttgaaac
    27481 cctcggtgac aaactcgttc tggatttcgg acattttctg ccgacaaagg aacgtcgctt
    27541 gcgtcatcgc gcgcgcatgc atgctgacgc gaatcgcacg ccccgttgtc aggtacagac
    27601 tgacgagcga caccgccaaa atcgccatcg cgatcatgac ttccagaagc gtaaatcctg
    27661 cctgcttgcg tcgcatcagg ggactccttt gtcgcttccg ctgacatcaa agtcgggcgg
    27721 tggtccttgc cttccgggca acacttcgac gcgcccggtc agcccgtgaa cccacaaggt
    27781 gtactgcacg tcgtcttgcg aatcggtgcc aaggtgaatc accgcccgct cggtgtgacc
    27841 gtcgggaaaa aagtgcagat aggcatgccc agacacatac ggatcttttt cgcgcggcaa
    27901 cagcacatcg agcactttga tgcgcgacag cttgaccttg ggcagcgtgc tgtctttgta
    27961 ttccgcaaac ttggggcgac ggggcgcttg cggaggcaaa agctcctcgg gaagaccgtt
    28021 tagggcgcgc ttgtcctctt ccgcggcttc cttgtcgagt tcatcgcgat ctttgccccg
    28081 gccacgcgac agcgtcgcca gcttggtgtc gataaggacg cgcgactcgg accgttccag
    28141 ccgataggtc tggttgtcga gatcaaagtg gagccgataa aacgccccgg tcgtcagcgc
    28201 acggtcgtag ctgtagcgga tcgcagcggc gagcttgctc gactgcgaac gcagctcact
    28261 gcggaccaga ctgcgcaggc ccatgaccgc cccgcccgaa atcaaaccga cgaccgccaa
    28321 cgcgatcatc agctcgacga gcgagtagcc agcctcggcg cgtcttgccg ccaaactatg
    28381 tcccccgttc cgctgcatgg gcctttggcg ttttacttgt cgtcttccca gctcttgatg
    28441 tcgtcggcgg tgccttcttt gccgtctttg ccaaagctcc acagatcgat gccgtcggtg
    28501 tcgtgctcgc cggggcactt catgacgaac ggacgacccc agccgtcttt gggctctttg
    28561 ttcaaaatct tggcggtcac caagtcattg agcgatccgg ggcactgctc tcccgtcgca
    28621 tccatccgcc actgaagcac cgcgtctttg actttgtgaa tcatcgtcac ggtttctttt
    28681 ttgcgcgctt tttcaaactg accaaacgcg acgacggcgg caccacccat caccaagctc
    28741 acaatcgcca gcgcgatcat gatttcggtc aagctgaagc ctccttcgac ccggcggagc
    28801 cgcggagccg aagtgtcgcg gcgcgcacga tgcgacaagc tctgggcgag ctggcgggcg
    28861 gcgcggcgca gtcggaacag aaccgaggtt tgcgaagcgg aaacaggggt tgggagcgtc
    28921 atgtgagtct ccttgtcgaa aacgggatct ttactgcaca aagtcgttca tctgcatgat
    28981 cggcagcatg atggacgtga cgacgaaaaa taccgcgcca cccatgccca agatcatgag
    29041 cggtgacaaa agcgcggtca gccgttccag ctgcgtctcg acttccaggt cataagcacc
    29101 cgagacgttt tcgagcatct gttccagctg acccgatcgt tcaccgacgg caatcatgtg
    29161 acacacaatc gccggaaact ggccactgcg cttgagcggc gccgcaatcg attcaccttc
    29221 gcggatcgac tcgcgcgctt gctcaatgac attcatcagc acggtgttgc cgagcacgtt
    29281 tttgacgatt tccatcgccc gcagaagctg cacacccgac gccagcatcg tcgacagcgt
    29341 cttggcaaaa cggctcaccg cgactttccg ttccaaacca ccgagcaccg gcacccgcag
    29401 cttgaacgtg tcccagctca gccgaccttt cggcgtcgac acccagcgac ggaacgcgac
    29461 aaagctacca atgccgatga tcagcaaaag ccacaggtaa tcgctgacca gcgtgctgat
    29521 gaagatcaat atctgggtgt tgagcggcag cgcccgtccc gtatcggcaa aaatcgccgt
    29581 cactttcggc accaccgtcg tcatcaaaag cgtcaaaatg ccgacggcga gcaccgtcat
    29641 gatgatcgga tacatcatcg ccgacatgac tttggcgcgc agcttgttct gcgattccaa
    29701 aaagtcagcc agacgcagca gcaccgcttc aagatttcct gcggtttccc cggcggtgac
    29761 catgttcacg tacagctcgc caaaacagcc cggatgccgc gccatcgccg atgacaaagg
    29821 cagaccttcg ttgacctgca ctttgacgtc ggacatcacc tgtttcagat tcggatcttc
    29881 ggcctgctcg atgagcgccg cgagcgattc cgaaagctgc acgccggcgc gcaaaagcgt
    29941 cccgagctgt cgcgtcagca ccgcaatttc catcggcttg gcggtgcgcc gatattcggc
    30001 aaacgcctgc gccacaaaac ccagccgcag cgcaacgagc agaccaacaa aaagaccctg
    30061 cccaactgtg tctagctgcg agtcactggc ttttttcttg gatttgccga cgccaacttg
    30121 ggcttcgctg acgtcggaca gcatgatgcc ttctcgcttg agcgcctgac gcaaagtccg
    30181 cgggctttcg gagtcgcgga ctccttgcac ctgcttgcct ttgccgtcgt agcctttcca
    30241 acgaaaaacc ggcatcgtcc tattccttgt cctcggcggt caccagcatc acttcttcaa
    30301 tggaagtaat gccagccaag accttgcccg cgccgtcggt gcgtagcgtc cgcatgccac
    30361 tgtgtatcgc cgcttttttg agcgtcgccg catcggcttt tttcagactc agctcgcgca
    30421 ccgcatcggt gatttcgagc agctcataaa tggccgaacg tccctgataa ccacgctgac
    30481 tgcacacgtt gcagccgcct tcgcgcgccc gatacagatg acccggcggc ggcggcggcg
    30541 ctttggtgat gtaggttgcg actttgtacc gcccggcaaa aaaatcttcg gcaggaatcg
    30601 ccagacgcga aagctcggtt gccgtcggct tgtacgtttc gcgacagtga atgcacaaac
    30661 gccgcacgag gcgctgcgcc agcacaccca tcagcgacga agtgaccagg aacggctcga
    30721 ttcccatgtc cacaagacgt gtcaccgcgc ccgctgcatc atttgtgtga atcgtcgaaa
    30781 acaccaagtg acccgtcagt gacgcggtaa tcgccatcga cgcggtttcc tgatcacgga
    30841 tttcgccgac catgatgacg tcgggatcgt gacgcagaaa cgatcgaatc gccgccgcga
    30901 acgtcagatc aatcttggga ttgacctgca cctgcgagat gccttcaagc tgatactcga
    30961 cgggatcttc gaccgtcagg atgttgatgt ccggcgtgtt gattttcgac agcgccgcat
    31021 acagcgtggt cgtcttgcct gaacccgtcg gacccgtcac cagcaagatg ccgtgcggac
    31081 gattgatcat ctcgaccatc gtttcgaggt gatccgcacc aaaccccaag tccgccagat
    31141 cgagcagcac cgaacttttg tcgagcaaac gcaccgtaat gcgttcacca aatgcggtcg
    31201 gcaccgtcgc gacacgcatg tcgatgtctt tgcccgcgat cttgcgtcgg atgcgaccgt
    31261 cttgcggcag ccgcttttcc gcgatgttca gccctgccat gatcttgacg cgagcgacga
    31321 tcgagttgat gaacgtgcgc ggcgcttttt tgtattcctc aaggacgttg tcgatgcgat
    31381 agcgcaccac catgtccttt tcgcgcggct caaagtggat atcggaagcc ttgacgcgca
    31441 ccgcatgaaa gatcaccgag ttgacccagc gaatgatcgg cgcttcgtcg ctgacatcga
    31501 c
//
LOCUS       DAORGN010000034        30985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_547_length_30985_cov_6.131102, whole genome shotgun sequence.
ACCESSION   DAORGN010000034 DAORGN010000000
VERSION     DAORGN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 30985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30985
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_30985_cov_6.131102"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKL17_06045"
     CDS             complement(<1..252)
                     /locus_tag="PKL17_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HNK44322.1"
                     /translation="MNIPASVQSWVNEIAQVTRPERIYFCDGSEAENRRLLDEMSQSG
                     VLLPLKSETYPNCYLHRSHPSDVARTEHLTFICSDSAEEV"
     gene            complement(414..1460)
                     /locus_tag="PKL17_06050"
     CDS             complement(414..1460)
                     /locus_tag="PKL17_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44323.1"
                     /translation="MSRLAFLLVGIGLCACGGTGLSSRPGDVPSSAHHNGERNPIGSP
                     VGSPVGSDSVSQMQSQTAIPITRNALSGTWDWLYRSSTQQGDLRIEEEEWNLHQKGSR
                     VSGDYLRQVTILSLDQKPFRCNGLFGFLLTSRVRLSGEVRGDAFVLQETSVEQEPSPC
                     SDEKMSPRSYEGSLLGNHLSLRYPGGQQTLVRRGKDSKVAPLTPLEGGGRESLSEKDL
                     VLNGIWDFQLRSSVDSDGDQHSEVEEWHLVDMGGKLAGYCDRIVERSRQRGVFACSGS
                     AKFRSVTRYTLRGRRSGEHFFLNETDYRSEPSPCENGGRRVDSYRGTILPMGQLLLEW
                     SGGQQILRRRREKD"
     gene            complement(1473..1997)
                     /locus_tag="PKL17_06055"
     CDS             complement(1473..1997)
                     /locus_tag="PKL17_06055"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNK44324.1"
                     /translation="MAANPSTEPGQNPDRAADSQTKAGDFSARAAYDRDSYSDADSDA
                     EYDDYDRDESPSYVPSISGRMFRLLGGLFSVHAEIAKQELGRDRSRILGGIILLVVGL
                     SCISMVILLLQGVLVWLFMRLGIALGFALLIVAGIDLLFAGIAIALSQRALARPVMPE
                     TRALLRRTIDSVIS"
     gene            complement(1987..2493)
                     /locus_tag="PKL17_06060"
     CDS             complement(1987..2493)
                     /locus_tag="PKL17_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44325.1"
                     /translation="MAEDTATRELSRTVFQDSFAKLQPVILAEWAELDPVSLSDTGGD
                     LDKVIALVAERTAHTKALVRKQLQELWVILSEPPRRRPPSGAPPSGGSARSQKGLHNV
                     LPESADELLRELEERTSHILKELRGGVLKDTRVKVRENLLFSLLVTLGLGFIIGVLFT
                     GWGPRRGR"
     gene            complement(2587..3552)
                     /gene="panB"
                     /locus_tag="PKL17_06065"
     CDS             complement(2587..3552)
                     /gene="panB"
                     /locus_tag="PKL17_06065"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNK44326.1"
                     /translation="MSVHSARRAKVTHLELRKMKEQGERIAMLTAYDFSFARLCDQAG
                     TDILLVGDSLGMVVQGQKNTLPVTMDHMIYHCQAVARGVEAASGRAMVVGDLPFMSYQ
                     SDLDEAMRNAGRLIKEGQAEAVKLEGGAEYCELVQRLNRVGIPVMGHVGLTPQSLHAL
                     GGFKIQGRDDQAAQRIKADAIALAEAGCFSLVLEGIPQELAQEITDSLQIPTIGIGAG
                     PECDGQVLVIYDLLGMNEEFRPRFVKRYENFAVRIRTAIDSYIGEVRSGAFPGPEHSF
                     SRSEKSKSAPRAVAAFGVVAESEGRVLDSVGVGCIPLRPTNHKPT"
     gene            3703..5298
                     /gene="hutH"
                     /locus_tag="PKL17_06070"
     CDS             3703..5298
                     /gene="hutH"
                     /locus_tag="PKL17_06070"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNK44327.1"
                     /translation="MSIVKPSETQASPFRIGEDVLDLTSMREICQQGRAVVLSDGAKQ
                     RIERSRAVVDKLVSGGDEAASAYGINTGFGFLADVRISARQIVELQYNLVRSHAAGVG
                     PRLPIPTVRGMLLLRACTLSLGHSGVRLSVAQRLCELLNRRIHPCVPSKGSVGASGDL
                     APLAHLTLGLIGEGDVEVGLPGNVSAKTAAQAHDEAGLTPIVLAAKEGLALINGTQCM
                     TSIGLLALADALDLCALADVIGAMSLEALLGTPRAFDPRIHAARPQPGQSISAQNLRA
                     LLHGSPIVESHKDCGKVQDPYSLRCMPQVHGATRDGLGYAKQVLLQESRSATDNPLIF
                     VEPGDALDYDIVSGGNFHGQPVALALDFAGIATAELANISERRIEQLVNPHLSSGLPA
                     FLVRESGLNSGYMIAQVTAAALVSENKILAHPASVDSIPSSAGREDHVSMGVHAADKL
                     QRIVENVRTVLAIELCCAAQGISLRAPLLPATALSAVYRVVRSRVPEYTHDRPLYQEI
                     EAVRTLCENGSLLAAARQHIELL"
     gene            5387..5722
                     /locus_tag="PKL17_06075"
     CDS             5387..5722
                     /locus_tag="PKL17_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNK44328.1"
                     /translation="MYASHVAASVRRSRPSWLVTIWALRIGAALSLAAFVGYVPYRLY
                     VRVGLQQHISLRRELRELTESNERLRRENGELRLQLLRVQEDDTEMERVARDELGLIH
                     SDELLFKVE"
     gene            5726..7993
                     /locus_tag="PKL17_06080"
     CDS             5726..7993
                     /locus_tag="PKL17_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNK44329.1"
                     /translation="MPARQVPSETTLQKWLLWRKHPRLVRGEWWALWAVLGLVLLLLA
                     NGQYRALGSGTAAISAVLCFVLLLLLMVLRLWPVQTGRDEGQPDRHMATGAAQLVLGF
                     ALAQGLGGLWGEAAVVRYALVAAVIGFHHGLRLLGLSVLTLCCEGLFRYAAGQHTPSE
                     ILGSLLILGLFMGLREAVFRGELLRQQMEHKQRMQAALLDIERQAELFRLNLTEDSLV
                     AATTEWTDHSGNESAADHTDRSEAEMLLLRSAVVMVRQNLDALVGLLRRALGLSTCAV
                     LWESGDGGVLEVVAASTDAPLPLLRAVSPSAGLLGSVRKTKAVQQLTAPKLAQLSYYD
                     RPVRPDDRVRFAAAMVLPLFEDGALTGMLCVDKMAREGEDAAPFSPSEEEALKAAVPV
                     ILRSIQSERVFRSVERGRDANRRLASASGQLTAALRPEDVQQSALSAVAKLCAYDFAA
                     LTEYDDQAKRHTVLAVSGDEAICAQVNGLRFAQNEGLCSSVIRRHTILPVGGILRDHG
                     ADTQVFDDHTRLRGYKWLLVLPLLLHHKPRGTLVVAGRSDAAAPERQPLLKVLANQIA
                     VSIDNARMYQAVESLATTDGLTGLYNRRTFQQRIEEMRERAERQSRTLALILCDIDHF
                     KKINDTRGHLVGDEVLRQVGKLLYETVRKVDVAARYGGEEFAILLEATEEEGARLLAE
                     RIRQEAEKLSLQSAKGPFSCTLSFGVATFPGDGVTTLDLIAHADAALYAAKHQGRNRV
                     VCYSVLADETAQPAA"
     gene            8090..9130
                     /locus_tag="PKL17_06085"
     CDS             8090..9130
                     /locus_tag="PKL17_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNK44330.1"
                     /translation="MPAPQLHLTFGDLVQHNPLVPLAMRRACAAEPVYARFGSIFHDL
                     PYYYAPMVFEAVRYGLGAPALDEPWGYRLHSVHPERLVESYIRAARTLPGLGDQERLA
                     LVGGLLSHCAIDLAMHPLVNYCARRDTLRHGGHESTHHRLTEKYQALFIHLWRLGDDQ
                     IGLPAFAERCRITKHGNSLSLKVEQPILDLMQQAFRGAYGSAPSVLQFSKWVRNFRQF
                     GILLSTPWVAKNSQRTRTDAIRERYFVNAEFDAREFFAAAERRLGKISTLSYDYFVAG
                     DFSPAAQASYLSQVGIDDLAEPQSVDLPQIPYLPPVRGKVQQPKQPKTPRIRLPQVRL
                     RRKKNPAATASM"
     gene            9292..11511
                     /locus_tag="PKL17_06090"
     CDS             9292..11511
                     /locus_tag="PKL17_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44331.1"
                     /translation="MIAVLSRTFTLGRYTCVERLGQSPVGEVWRAKRFGLAGLDRQYL
                     VYRIGASQIAQDSTANLRLQAALRSVSEIQHQNLLHIEEFGSQSGDQFVAYEFVGFAD
                     LGKLKSGIDLLGDEAKSLLPQIVALIGQQLARALLAAQERGVMHGMLSPSCIWISPGG
                     DAHIAELGLWAVLPQGPWKLDGALKSLGPYVAPELLDSGVPGPRSDVFALGSLLAEQL
                     KLVSQDKLSPDSKSAQSSLQTICQRAMEKSAGQRPSSMLELIKQLDAVRVTEAAPAQL
                     ARFGQLFELAGDTVPQPVTTSDRSAAEPLPPPPSMKIAVGKTPAKPPEKAAAKDAVGS
                     EKAAPQDKADAADGKAAASPVTINPRAAFGAGMAGNGASAVTPTADVDAAEAELAGGD
                     GKNKPALPARLRPSTPPPDTDHISVTDTNPAMMPLPKAKRTTAKPSKAVGDEPTNQFK
                     LEAAEAALAAEQAAAAASAPAADPPVSDPSTLEASELLEENPALNSTGNYSQPELPPV
                     TPEIAAAAGMSQSPSGSPSAPAVEPSPPPESQPRRLSPAAFAGIGLGAAVVAGGIMFA
                     LIGGNSTSNSVISDGGAKDGMTSTQDVVAKLPSDEIQVDSAPSAAVFLDGESKGKAPL
                     KLKVKAGTHKLLIVADSFKLWKQEASGGQTISAKLIKASLPDDVSGTAAVKVKCKRDG
                     ELRILVDGNDSGLSCPTDDLMLKPGKYTLGFLSPTSDELREKSIKVKKKGLKLKVKF"
     gene            complement(11556..16115)
                     /locus_tag="PKL17_06095"
     CDS             complement(11556..16115)
                     /locus_tag="PKL17_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK44332.1"
                     /translation="MTPSAPPLDVAQTLEGKRVLFIGGTGFVGKVTMSMLLCKYPMLG
                     KLYALVRPGSGYTAETRFFQKIAKSRPFDPVWKTFGDGTEKYLREKVVPLAGDVSRPN
                     VGLSDADLALLTQDGPLDVILNSAGLVSFNPSLESALRINVHGVRYVLDLARATSAKV
                     VHVSTCFVAGRREGEVWEDESLIGYFPRRPGHDRSLSAGSALRKGDFDPEAEIADCER
                     LIEQTRQRSEDRAHLSMFREQGADRLRQEGRDPDDERHLKAALQREKKTWTAERLTEL
                     GMERAQHWGFTNTYTYTKSIGDQLCAMATLPESERKDGKKPVRVAIVRPAIVESAMSY
                     PFPGWNEGFNTTAPLIFMVLKGHIQVPASPGTVLDMIPVDMVASATIAATAATLAERN
                     ELIYHLGSSDSSPLRMPRAVELTGLYKRRHFREKLEHKQGDAVMNRLRSRLESIPVSK
                     TRYQKLSVPLLRGVAEAASGMIGDALDKMWGMPRLTALGERAREKLDETAQLAKQAEG
                     IFDLFFPFVHDNAPIFRADNTRRLFRSLTPHDQTLLRWNPETINWREYMLDVHIPGLE
                     KWIFPSLDEEFQARAKAVYTYRDLLEMLESAAKAYRGRTAMRLLPPLDADGEPIGHGQ
                     RYTYRDLSERSAKVSETLRRLGLPVSGKVLLLSENRPDWAISYFGILKCAAVVVPVDK
                     DASLEEVLNICRFADVYAAIVSDKVQARIDVRGALAQTMPSVKVLNFRELFSAETPHA
                     LVAASSLPALTLSGRADEMASLIFTSGTTGRPKGVMLSHRNFTSLLAKMGGVFDVDKH
                     DGLLSVLPLHHTFEFSAGLLMPMSRGAQIAYLPEVNADSLQNAFSSAHITGMVGVPAL
                     YQLLYRRITKQISDRLPQRAVPWVLRLLERLYDLTRSARERTRKALGIELNLPRALFL
                     PVHQRFGGKLRLLISGGSALPIETMKEFRGLGFNLYEGYGMTESAPVLTVTRPGSPLL
                     LGSVGEALPGVDVKIHEPDATGTGEIIASGPNVMLGYYNDPELTKETIKNGYLHTGDL
                     GRIDGKGHLFIVGRKKDVIIGANGENVYPDELEERYRESPFVKELCVVGLAEAELKEN
                     PSPDDKGEAVACLIVPAYDAPQSEGLSREAVREKVREHVRNVSAKLSVAKRVKILHLT
                     DLELPKTATRKVKRKQVVDELRRLERTRRQAQAEVQSGGTATAGDWLLSLLADVTGKP
                     RSAVRPESTMQELGLDSLSFAELGVALEAAGVQVPENVDITSIVSVPELQKAIAQWGR
                     RKPVKEAPKEKTKKKDKNESTDDRLVLPDWLVRAGNRGLNAGQRALYERVLKTRVTGK
                     AYLPASRQFIVAANHSSHLDMGLVKHALGDWGDRLVALAAKDYFFDDPLKRVYFENFT
                     NLIPMDRTGSLRESLRLAARVIEDGYVLLIFPEGTRSESGIMQSFKASIGYLALHHQI
                     DVLPMYLEGTHDAMPKGNVLPKQGTPVAAHIGPVLRFEQLQKAVAKVPRTEQNREAAR
                     LVELAVRKLAPVGPNRQMPKDADVE"
     gene            16250..17671
                     /locus_tag="PKL17_06100"
     CDS             16250..17671
                     /locus_tag="PKL17_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNK44333.1"
                     /translation="MKSACSLLSQSAARSHRFLLGTFVFALCSCTHGNQGIELGGNIP
                     PSVGTEILGLPPIDPNLQYNHTFVQQTEADFFGWSKNGIVASRDGNRVRLVLSPEKQG
                     TLRCESVDIDGGMARFDAASGLCAGTDPMPGGLPSGVNYYSGGSFFFGTMTSPEVTPR
                     APIDHLIASWNAQTPPGTWLQLRVRMKVGGAWSRYYKLPIWSSDAAVVKRHSIKEAAD
                     DTGYVDTDTFELRNQKTATAYQLSVTLFSTDAAKTPALGLVAAVASRDKTDYPKQDLD
                     PSVFGKNLPVPQRSQELPEYKKPEYQQYGGGGEVWCSPTSTSMVMEYYSQKLGDPQLN
                     QTVPDAAIGCYDWVYKGTGNWPFNTAYASTLGLAGYVTRFYSFAHAAPFIKAGVPLII
                     SIAFKAGELPGAPISKTNGHLIVVRGFDKNGDVIANDPAARDNASVQLVYPRAALEAA
                     WAHSHRTAYLLYPPTGFDLPQLP"
     gene            complement(17690..19189)
                     /locus_tag="PKL17_06105"
     CDS             complement(17690..19189)
                     /locus_tag="PKL17_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNK44334.1"
                     /translation="METQHAIVKSYVAAHQPVTQEVQAVLPISEGRLPSDLRGVLFRN
                     GPGRFANHGQTYGHLIDGDGMVIRFAIGSHGPDGQPCIRYRNSYVRTTEFVYEQRSGR
                     LLYRNFGTNRAGGFFGNLFRTHVKNVANTSVIYHHGRLFALWEGGLPHLLDPVNLDTI
                     ERYDFFGALKNRGPLLERVLLPELPFSAHPKEDPNTGELWNFGLYLGPHPKILLYNIR
                     GGVALTVTREIPLPAPLLAHDFLLTPNWQVLFLFPVSLRMLPTLMGRPPSGSVQEVEG
                     GMTTILLIPRDGGPVKKFQASPCFVLHFVNAYEDENGALVVDACRQDHFPDAPRPGEL
                     LAGHISDIPHVKLTRYVIDPSAEEGTAVSESPLGGFSSELPRIHPARQGLPYRYAFTV
                     SAMPDAPHGLFSAIARFDVQTGDMQLRDFAPGIPGEPVMVPRPSAQDEDDGWLLTVVY
                     HGSEHRSALYVLDARDLSTVCVAELPHHIPPGFHGNFVPAAHCPPGWHS"
     gene            complement(19328..20416)
                     /gene="nagZ"
                     /locus_tag="PKL17_06110"
     CDS             complement(19328..20416)
                     /gene="nagZ"
                     /locus_tag="PKL17_06110"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNK44335.1"
                     /translation="MPSPAELFIVGYQGTELPTSLATRFRRGDFAGLIFFRRNFQNPD
                     SARAIAIDMESVLRGYAPSSAVEQAAPILAIDHEGGRVQRLRGDVTVWPPMHKLADKP
                     EQLCEAVGQAMGWELAALGFNVSFAPVLDVHTNPANPIIGDRAFGTLPEAAASRALAY
                     LRGLERNVGLRGCGKHFPGHGDTETDSHLTLPVVKRDLATLRQVELLPFERASAADIG
                     MLMTAHVVYPAVDARPATLSRKWLTDMLRGELGFSGVVVSDDLDMKAVTAQQLGIADD
                     SGVVVESLLAGCDAFLFCQDAERLLRAEEALSRAAEKSSIVRDRIAESAARLSRFRAT
                     LRSSGPTHERIAHFPVQDHLLLTSQVLV"
     gene            complement(20400..21152)
                     /locus_tag="PKL17_06115"
     CDS             complement(20400..21152)
                     /locus_tag="PKL17_06115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNK44336.1"
                     /translation="MATRARLFVSPDRLGPSVVLRGAEHHYLASVLRLRPGAQVTLLD
                     GQGRQALAVVSAVHAQTIELSAEPPTHVSVAQPTLVLFVGLLKGDKQDFVIQKATELG
                     ASRIVPVQCDRSVPQLLGERSAAKHRRWQDIAKGAAQQSKRADVPDIAQVQTLEVSLR
                     EAEGQKLLFYEGCAPPLAQVLDPGAAPKVVSLLIGPEGGLADSEIEAAKAVGFSSVSL
                     GPLVLRAETAVVAALAVVGYLLSQGADDAKSC"
     gene            complement(21171..24971)
                     /locus_tag="PKL17_06120"
     CDS             complement(21171..24971)
                     /locus_tag="PKL17_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44337.1"
                     /translation="MAAPRPVGVSLLPKGVAAPLAKVALSPLPSLAGPNQSTTVAPPI
                     PAASTAIPPGISPLPLPLPLLAQAQNKPTTTATPDPEDPQRFATPSPGQVVDEEDEES
                     EEEKKRKREEAKKAKPECDSDAQCPDKTVCIKQSCQSVNRPLSAILYFHRKGPIGYRL
                     VLPFYYSFWRPDRTTKVLFPLFADRKDDKEKTRDTWVFPTYQYKREPGMRAHRIWPIF
                     FYQSYGDSGEKGKSVGLLPLFWVTRRTDAQTVVLPPLLFWHHHNVEEKRTDTGFLPLL
                     LFVRKDPELSQAFFLALGFYRKTPDRTVGGFLPLVFHSKTAEKRHTMVLPLFYDGENY
                     QTGTRTSTLFPLYLYHRGADKSRLLVTPLGGSYRNPEEESTTTVLLTPPTFHRDDPVR
                     RFTTVLPPLAMWYRNKTTGAAWGYTGPFFFTRDEEGGSEGLLPLYLHFFSKRAQSDTH
                     VFVPALAALHTSPTRKFGFLGPVYGWSSKTGSGGGLLPLISVARGDKPHVAILPPLFI
                     YAADKEAGSSYVSVGPVFARVKTRGEDAGYTAGVFPLLWLHRRGQSSTQMLFPLLYHH
                     RRPHEETLQLGPFYWYRKCPEYIGDKRAEVRAGLVPLLFWKRSPESSYTVLFPLLWQV
                     RNPRGSALVIGPFFHTTEKRPEGEVKTTGLVPLFYASQGPKSTFAIAPLFAYHRTPES
                     KTVWVGPYVETVRGLGTREQVINRAVLPLYFFHCSPGRRASVLFPLFADVKDKDTEFR
                     QIALLYYGVKTPERTAHVLLPLFFHVRSPERTSTVLLPFFYSKNEKTGGVSAGLLPLF
                     AFGKSKEATWATTPLGFFYKDEKATRAAALLFYADVRKDREDFGVFPLWFYSRRDTAH
                     GLFILPFIYHSHDAARSRHLTVLGPLYFGRRGEATFGGFAPLFYGRNDGDGSYRFMAL
                     PLVYFSHRAGKAPEDWLITPLVGVNRNPVGFRFWIGPLYVRKQDPVTSAALFPLFYYT
                     RDDKAHTSTSMLLPLWLQTRSPERSLTMATPLFWHERTLTRRITTFFPFVLDVHHLHS
                     DRLTAVGPLVPLVVRLSDYAAKTTTWVFPPLLTYVKKRADGYHNAVVFPLVWHFGGKD
                     RSTTVLFPLFYHVRRPASQFTALLPFFAYRRDEQGTKTLFLPPLLTWVRNYEDGSRDR
                     VVFPFVWHFKRPKQTTTVFFPIGAHWANEKGHYTLVLNSYYYKGTGERAGAYRFEFWP
                     LFHVGRPRAGDLEWSILSGLVGYSRDGIRRTLRLLWGVFIPLEPVGAQASWYGASWRM
                     ASDR"
     gene            complement(25043..25543)
                     /locus_tag="PKL17_06125"
     CDS             complement(25043..25543)
                     /locus_tag="PKL17_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44338.1"
                     /translation="MSSVSAYASEVVTLADNTQIAGKLTHYFDGIIVMETSGGQKLEL
                     PRDKVKSITFKLPPPRAEFSTPEKTFERWRGAMAKGDVQKAIDCYAMLYQGQVGQQMM
                     QSPDELKKAQKDLEGVQFELRGSTFKAQGEFKLATLKVRRSKGENVQTDEVNLVLENG
                     EWKMTP"
     gene            complement(25766..27430)
                     /locus_tag="PKL17_06130"
     CDS             complement(25766..27430)
                     /locus_tag="PKL17_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK44339.1"
                     /translation="MTQVEIFVPLQQLQLDEDEAVHADPETALWAVAMLEAARRAGIP
                     RPGGARLLRKSLDARKGFPIGYRMQIEVFPEGAELPPACSRIDLRRPGGKGSWPFLQG
                     GMQRLSPPASLTEKKRVVVVGSGPAGTMCAKRLCEAGADVILIEQGQPVQPRRHDLAK
                     LTRGELSSDSNYCFGEGGAGTFSDGKLYTRSKDRAAIGETILTLIEHGADPRISVESR
                     PHIGSNRLPKILLELRADLTARGVQYHFGEKVTELLRAPSGKLLGVRCASGLSVLADA
                     VVLAVGHSARGIYELLVRQDILVERKPFAIGARVEHPQPLIDRIQYGRAAEHPALPAA
                     FYQVAASVGERGVYSFCMCPGGWVVPCATEPGGLCVNGMSLKRRDSPLANSALVVSVE
                     DRDVAPFERFAGDPLAGMWFQRAVEERAFLAGGGRFVAPAHKLTDFLVGKPSSDVLPT
                     TYRPGIVSANLADVLPPFVVDSLRQAVQRFGRTLPGFVSPQAQLVGVETRTSSPLRIV
                     RDDKLMSPSLAGLFPVGEGAGYAGGIVSAAIDGLRSADAVISYLRS"
     gene            complement(27445..28479)
                     /locus_tag="PKL17_06135"
     CDS             complement(27445..28479)
                     /locus_tag="PKL17_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK44340.1"
                     /translation="MQKQSSTETKQHEPTFRQRGRAFALLVGVSLAALGCGDGTALPD
                     SEQPAQLAQPLSCKGAPYPIVLAHGFAGFERIGPLNYFFQVGADLRSRGETVVEAKVP
                     PYESSDVRAAALAAVVDDTLKTYNACKVNVIGHSQGGIDGRALISGKKYGNKVASLVT
                     VSTPHRGTEIADVVSGLVPGFSYDVINAILKAVSGLTDAPGSPNLKNALAQLTTPGMQ
                     RWNAANPDDPAVRYYSVAGRSAGRIANSECAGGAWGNSSRIDLLDPLLALPEGVFAIY
                     SDNPLVPRVNDGLVTVQSAKWGKFLGCVPADHLDEVGQIGHLFPDVVSGFSHKELYRK
                     IVAELRKDGL"
     gene            28578..29816
                     /locus_tag="PKL17_06140"
     CDS             28578..29816
                     /locus_tag="PKL17_06140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44341.1"
                     /translation="MSQDQTVNDPLIGTVVDRRYKVLKRLGVGGMGIVYKVEHSFLNK
                     FFALKVMRPTKDETDRKRFEQEARLASVIRHPNVVEISDFGVLPTNQPYYVMEFLSGQ
                     TVGDAIFAGFMDSQLVCHIGVQIARGLQSIHKQNVVHRDLKPDNIFLIDPDKTGENQD
                     ENSETGDSEPAFVKIMDFGIAKSVNSHLTGTGMTVGTPEYMSPEQARGETVDWRSDQY
                     SLGCILYEMLTREIPFDGKGAFEVMYKHLNAQPVPPRKRRPERADFISSELEAIVLKM
                     LAKKPKDRHPSMRVVELALRDEAARLLLSGAGDDTMVGSTPVHVSNDSTPLIRTLEDT
                     RKTVPFMPVAAPPETMANRPTKKIDIKDQAEMMNLIGRIGGIVHSPRSLPPASVQPGV
                     FQSILAWFARLWQRLARLWK"
     gene            29826..30188
                     /locus_tag="PKL17_06145"
     CDS             29826..30188
                     /locus_tag="PKL17_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44342.1"
                     /translation="MTYGELWWRAFWMTQAIEVPIYLLVLTKFPARPRWRSGREPVTD
                     WPHKFFCAFLASAITHPWVWYFFPYVIDDYWPMVAVAETFAVVVEALWFHFCGLRRAL
                     LWSLIANGTSATIGFLRH"
     gene            30223..30579
                     /locus_tag="PKL17_06150"
     CDS             30223..30579
                     /locus_tag="PKL17_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44343.1"
                     /translation="MSDWLYRTPTAFHGKKLHRLGLACSYGIDEDGVRAGMDRGVNFF
                     LWTMKNKAMRAPLGDAFKNRRDQVAVIGYATLGFFGWGVRHSCESLLKELKLDYLDVY
                     LLGWLGVTTAYTAATE"
     gene            30688..>30985
                     /locus_tag="PKL17_06155"
     CDS             30688..>30985
                     /locus_tag="PKL17_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNK44344.1"
                     /translation="MIRYNAAHPGAERDIFPHVKAQKPSILAYTATRWGKLLKRPSGY
                     DGPVMTAGDCYRFQLSNPHVDIALTGPASRAELIQNLDALDKGPLTPEEDAWMRS"
BASE COUNT         6203 a         9763 c         9245 g         5774 t
ORIGIN      
        1 gacttcttcg gcgctgtcgc tgcaaatgaa cgtcaggtgc tccgtgcggg cgacatcgct
       61 gggatgagag cggtgcaaat aacagtttgg atacgtttcc gatttgagcg gcaagagcac
      121 gccgctctgg ctcatttcgt cgagcaagcg gcgattttct gcttcggaac catcgcaaaa
      181 gtagatgcgc tcagggcggg tcacttgggc gatttcgttc acccagcttt gcacagaggc
      241 ggggatgttc atggggtttt ggtcctttca aatcccgacg ggccgattgt cgaccacgcg
      301 ggcttgtcgt ctaagcgaag gcaagctgtc cgaatcgagg acgcccggaa cctaccacaa
      361 accccgccgg aaccgcgagt ggagaccgcg acaacacgac cagcgcagtc gggtcagtct
      421 ttttcgcgac gacgccgcag gatctgctgg ccgccgctcc actccagcaa aagctgaccc
      481 atgggaagaa ttgtgccgcg ataggaatcg acccggcgtc cgccgttttc gcaaggactt
      541 ggctcgctgc gatagtccgt ttcattgagg aaaaagtgtt cgccgctgcg tcgcccacgc
      601 aaggtgtagc gcgtcacgga tcggaacttc gcactgccgc tgcaagcaaa cacaccgcgc
      661 tgccgactgc gttcgacgat gcgatcgcag tagcccgcga gttttccgcc catgtcgacc
      721 aggtgccact cttcgacttc gctgtgctga tcaccatcgc tgtcgacaga gctgcgcagc
      781 tgaaaatccc agattccgtt taagaccaga tccttttcgg aaagcgactc gcgcccaccg
      841 ccttccagtg gtgtaagcgg tgccactttg ctgtctttgc cacgccgaac aagcgtttgc
      901 tgaccacctg gatagcgcag cgacaggtga ttcccaagca gactcccttc atagctgcgc
      961 ggggacattt tttcgtcgct gcacggcgac ggttcctgtt cgactgatgt ttcctgtagg
     1021 acaaacgcat cgccgcgtac ttcaccgctg agccgcacgc gactggtcag caaaaaacca
     1081 aacagaccat tacagcgaaa cggcttctga tccaaagaca ggatcgtcac ctgccgcaga
     1141 tagtccccgc ttacgcggct gcctttttgg tgaagattcc actcttcttc ctcgatgcgc
     1201 agatcccctt gctgggtgct gctgcggtac agccagtccc aggttccaga cagcgcattg
     1261 cgggtgatag gaatcgcggt ttgggattgc atctgagaga cagaatcgct gccaaccgga
     1321 ctcccaactg gactcccaat cggattcctt tcaccattgt gatgtgcgga agaagggaca
     1381 tcgccaggac gactggaaag accggttccg ccacacgcgc agagcccgat tccgacgagc
     1441 aagaaagcaa ggcgtgacat cacaaacgct cgctaggaaa tcacagaatc gatggtccgc
     1501 cgcagcagcg cccgcgtttc tggcatcacc ggccttgcca gcgcgcgctg cgaaagcgcg
     1561 atcgcgattc cagcaaacag cagatcgatt cccgccacaa tcaggagcgc aaaaccgagc
     1621 gcgatgccaa gccgcataaa gagccacacc agcacgccct gcagaagcag aatcaccatc
     1681 gaaatgcacg acagaccgac gacaagcagg atgattccgc ccaggatgcg ggacctgtcg
     1741 cgaccgagtt cctgtttggc aatctcggcg tgaacggaaa acaaaccacc gagcaaacgg
     1801 aacatccgtc cgctgatgct gggaacatag gaagggctct catcgcgatc gtagtcgtca
     1861 tattccgcgt cgctgtcggc atcggaatag gaatcgcggt catatgccgc tcgcgcagag
     1921 aaatcgcccg ctttggtttg ggaatcggcg gcgcgatccg ggttctgccc cggttctgta
     1981 ctgggattag cggccacgcc gtgggcccca gccggtaaag agcacaccga tgataaaacc
     2041 gagtcccagc gtcaccagca gcgaaaacag cagattttca cggactttga cgcgggtgtc
     2101 tttgagcacc ccaccacgca gctctttgag gatgtggctg gtccgttctt cgagctcgcg
     2161 cagaagttca tccgcggact ctggcaggac attgtgcagt cctttttgcg atcgcgcaga
     2221 tcccccagaa ggcggcgctc ccgacggtgg acgacgacgc ggcggttccg acaaaatgac
     2281 ccacagctct tgcagttgct tgcgcacgag cgctttggtg tgcgcggttc gttcggccac
     2341 aagcgcaatc actttgtcga gatcgccgcc ggtgtccgaa agcgaaaccg gatcaagctc
     2401 cgcccactcg gccaagataa ccggctggag ctttgcaaag gagtcctgaa acacggttcg
     2461 gctcagctcg cgggttgcag tgtcttcggc catgacttcg cccctctatt cttcagaaca
     2521 tcacagacca aaaggaagcg gatgggaatc ccttttgtgt gacacccacc gcttccgggg
     2581 ttttgtttag gttggtttgt ggtttgttgg acgcaagggg atgcagccaa cgccgacgct
     2641 gtcgagcacg cggccttccg attcggcgac gacgccaaag gcggcgacgg cccgcggtgc
     2701 cgacttgctc ttttcgctgc gcgaaaacga atgctcggga ccggggaagg cgccactgcg
     2761 gacttcgccg atgtagctgt cgatggcggt acggatgcgg accgcgaagt tttcgtagcg
     2821 cttgacgaag cggggacgga attcttcgtt catgcccagc aagtcgtaaa tcaccaaaac
     2881 ttggccgtca cactcgggac cggcgccgat gccgatggtc ggaatttgca gcgaatcggt
     2941 gatctcttgc gccagctctt gcggaatccc ttcgagcacc agcgaaaaac agcccgcttc
     3001 ggcgagtgcg atggcatcgg ctttgatccg ctgggccgct tgatcgtcgc gaccctggat
     3061 tttgaaaccc cccagcgcgt gcagcgattg tggagtaagt ccaacatgcc ccatcaccgg
     3121 aatgccgacg cggttgaggc gctggaccag ttcacaatac tcggcgccgc cttcgagctt
     3181 gaccgcttcg gcctgccctt ctttgataag ccgtccggcg ttgcgcatgg cctcgtcgag
     3241 atcggactga tacgacataa acggcagatc gccgaccacc atcgcacgac cactcgccgc
     3301 ttcgacgccg cgcgccaccg cctgacagtg atagatcatg tgatccatcg tcaccggcag
     3361 ggtgtttttc tggccttgga cgaccatccc gagcgaatct ccgacgagga gaatgtccgt
     3421 cccggcctga tcgcagagcc gagcaaagct aaagtcatac gccgtgagca tggcaattcg
     3481 ttcgccttgc tccttcattt tgcgcagttc gagatgcgta accttggcgc gccgagcact
     3541 gtgtaccgac atgtgggcct ccttgccgtc tcggtcctgc gatgaagatc caagcggcag
     3601 acatgggcaa ttgcggggga actctttatg gattccacac agccacggcc cgcagcaagg
     3661 gggaagccgc gaaaaagatg catttgtgtt acgaagcggc gcatgtcgat cgtaaagccg
     3721 agtgaaaccc aggcttcgcc gttccgcatt ggcgaagacg tgctggattt gacgtcgatg
     3781 cgggaaatct gtcagcaagg ccgcgccgtg gttctgtccg acggggccaa gcagcgcatc
     3841 gaacgatcac gcgccgtcgt cgataagctc gtgagcggcg gcgatgaagc cgcgagtgct
     3901 tacggcatca acaccggctt tggctttctg gccgacgtgc gaatttccgc ccggcaaatc
     3961 gtcgagcttc agtacaactt ggttcgctcg cacgcagccg gcgttggtcc acggctgccg
     4021 attccaaccg tgcgcgggat gctgcttttg cgcgcctgta cgctgtcttt gggacacagt
     4081 ggcgtgcggc tgtccgtcgc gcagcgcctg tgcgagctgc tgaatcgtcg catccacccg
     4141 tgcgtgccaa gcaaaggttc cgtcggtgca tcgggcgatc tggcgccact tgcgcaccta
     4201 acgctcggac ttatcggcga aggcgatgtc gaagtcggtc tgcccggcaa cgtgtcagcc
     4261 aaaacagcag cgcaagcgca cgacgaagcg ggacttaccc cgattgtgct cgcggcgaaa
     4321 gaaggtctgg cgctgatcaa cggaacgcag tgcatgacat cgattggtct gctcgcgctc
     4381 gccgatgcgc ttgatttgtg cgcgctcgcg gatgtgatcg gcgcgatgtc gctcgaagcg
     4441 ctcctcggga cgccacgcgc tttcgatccg cgcatccacg ccgcccgtcc gcagccgggt
     4501 cagtcgatct cggcgcaaaa cctgcgcgca cttttgcacg ggagcccgat cgtcgaatcg
     4561 cacaaagact gcggcaaagt ccaagatccc tactcgctgc gctgcatgcc gcaggtacac
     4621 ggagcgaccc gcgacggact cggctacgcc aagcaggtgc ttttgcaaga atcgcgcagc
     4681 gccaccgaca atccgctgat cttcgtcgag ccaggggatg cgcttgatta cgacatcgtg
     4741 agcggcggca acttccacgg tcaacccgtc gcgctcgcgc tggattttgc tggcattgcg
     4801 acggcggaac tagccaacat ttcggaacga cgcatcgagc agctggtcaa tccgcacttg
     4861 tcgtcggggc tgccggcctt tttggtacgc gaaagcgggc tcaattcggg ttacatgatc
     4921 gcgcaagtga cggcggcggc gctggtatcg gaaaacaaga tcctcgcgca tccggcatcg
     4981 gtggattcga tcccatcgtc ggcaggtcgc gaagatcacg tctcgatggg tgtgcatgct
     5041 gccgacaagc tccagcgcat cgtcgaaaac gtccgcaccg tgctcgcgat cgagctgtgc
     5101 tgtgcggccc aaggaatttc gctgcgtgct ccgctgctgc cagcgacggc actttcggcg
     5161 gtgtatcgcg tcgttcgctc gcgcgtgccc gaatacaccc acgacaggcc gctctatcag
     5221 gaaatcgagg cagtgcgcac gctgtgtgaa aatggcagct tgctcgccgc cgcccgtcag
     5281 cacatcgaac tgctctgatc agcgacgatc ggttcatcac ccccgacaga agccgggccg
     5341 cgcaaaaatt tgtgtttgac tcggcctcca cgcaaaatcc cagccaatgt acgcttccca
     5401 cgtcgcggct tcggtacggc gctctcgccc gtcgtggctc gtcacgatct gggcgctgcg
     5461 catcggagca gcgctgtcgc tggcggcttt cgtcggttat gtgccctatc ggctgtacgt
     5521 ccgcgtcggg cttcagcagc acatttcgct tcgtcgcgag ctgcgcgaac tgaccgaaag
     5581 caacgaacgg ctgcgtcgtg aaaatggcga gcttcggctc cagctattgc gcgtgcagga
     5641 agacgacacc gaaatggagc gcgtggccag ggacgagctg ggacttatcc acagcgacga
     5701 gcttttgttc aaagtggagt aagctttgcc agcgcgccaa gttccgtcgg aaacgacgct
     5761 gcaaaaatgg cttctgtggc gcaaacaccc gcggcttgtg cgtggggagt ggtgggcgct
     5821 gtgggccgtg cttggtctgg tgctgctgct gctcgccaat gggcaatatc gcgcgcttgg
     5881 atcagggacg gcggcgatct ctgctgtgct gtgctttgtg ctgctgcttt tgctgatggt
     5941 gctgcggctg tggccggtcc agacgggccg cgacgaaggc caaccggatc ggcacatggc
     6001 gacgggggcg gcgcagcttg tgctcggttt tgcgctggca caaggactgg gcggactgtg
     6061 gggcgaagcg gcggtggtgc gttacgcgct cgtcgcggcg gtgattggtt ttcaccacgg
     6121 actgcggctt ttggggctgt cggtgctgac gctgtgttgc gaaggtcttt ttcgctatgc
     6181 cgcaggtcag cacaccccaa gcgaaatcct cggttcgctg ctgattttgg ggctgtttat
     6241 gggtctgcgc gaagcggtgt tccgcggcga gcttctgcgt cagcaaatgg agcacaagca
     6301 aaggatgcaa gcggcgcttc tcgacatcga gcgacaagcc gagctgtttc gtctgaacct
     6361 gaccgaagac tcgctcgtcg cggcgacgac agaatggacc gatcacagcg gcaacgaatc
     6421 ggcagcggat cacacagacc gcagcgaagc ggaaatgttg ctgttgcgga gcgccgtcgt
     6481 gatggtgcgg caaaacctcg atgcgctggt tgggcttttg cgtcgggcgc ttgggctgtc
     6541 cacctgcgcg gtgctgtggg aaagtggcga tggaggtgtt ttggaagtcg tcgctgcgtc
     6601 aaccgatgcg ccgctgccgc ttctgcgtgc ggtgtcgcca agtgccgggc ttttgggatc
     6661 ggtgcgcaag accaaagcgg tgcagcagct gacggcgccc aagctcgcgc agcttagcta
     6721 ctacgatcgc ccggtgcgcc ccgacgacag ggtgcgcttt gccgcggcga tggtgctgcc
     6781 gctgtttgaa gacggtgcgc tgaccggcat gctgtgcgtc gacaaaatgg cccgcgaggg
     6841 ggaagatgcc gcgccgtttt cgccaagcga agaagaagcg ctcaaagccg cggtgccggt
     6901 gattctgcgc agcattcagt ccgagcgcgt gttccgcagc gtcgagcgag gccgggatgc
     6961 aaaccggcgg ctggcgtcgg catcggggca gcttacggcg gcgctgcgtc ccgaagacgt
     7021 ccagcagtca gcgctcagcg cggtggccaa gctgtgtgcc tatgactttg cggcgctgac
     7081 cgaatacgac gaccaggcaa aacgccacac ggtgcttgcg gtcagcggcg acgaagccat
     7141 ctgtgcgcaa gtgaacggac tgcgttttgc ccaaaacgaa gggctctgtt catcggtgat
     7201 tcggcgtcac accatcctgc cggtgggggg aattttgcgc gatcacggcg ctgacaccca
     7261 ggttttcgac gaccacaccc gcctgcgcgg ctacaagtgg cttttggttt tgccactttt
     7321 gctgcatcac aagccgcgcg gcacactcgt cgtcgcgggt cgaagcgacg ccgctgcgcc
     7381 cgaacggcaa ccgctgctca aagtgttggc caaccaaatt gccgtctcga tcgacaacgc
     7441 gcggatgtat caggccgtcg agtcactcgc gaccaccgac ggactgaccg gactttacaa
     7501 caggcgcacc ttccagcagc gaatcgaaga aatgcgcgag cgagccgaaa ggcagagccg
     7561 cacgctggcg ctgatcctgt gcgacatcga ccacttcaaa aaaatcaacg acacccgcgg
     7621 ccacctcgtc ggggatgaag tgctacgaca ggttggcaag ctgctttacg aaaccgtgcg
     7681 caaggtcgat gtggcggcac gttacggtgg cgaagagttc gcgattttgc tcgaagcgac
     7741 cgaggaagaa ggggcacgac ttttggccga acgaatccgc caagaagccg aaaaactgtc
     7801 gctgcagagc gccaaaggcc cattttcttg cacgctgtcg ttcggtgttg cgacattccc
     7861 cggtgacgga gtgacgacgc ttgatctgat tgcgcacgcc gatgcggcgc tctatgccgc
     7921 caaacaccaa ggccgcaacc gggtcgtctg ttacagcgtg ctcgccgacg aaacagccca
     7981 gcccgcagcc tagaattgcg ccactggccg gaccatcatc cctgatttgt cgatgttgcg
     8041 acggagatcc ccacgccgaa gtggggggct tgctgctaga atcggtcgca tgcctgcgcc
     8101 gcagcttcac cttacgttcg gcgatctcgt tcaacacaac ccgctcgtcc cgctcgcgat
     8161 gcgtcgagcc tgtgcagcgg aaccggtcta tgcccgcttc gggtcgattt tccacgacct
     8221 gccctactac tacgcaccga tggtgtttga agccgtgcgt tacggactgg gcgctcccgc
     8281 gctcgacgag ccgtggggtt atcgtctgca ctcggtccat cccgagcgac tggtcgaatc
     8341 gtacattcgc gccgcccgca ccctgcctgg acttggcgat caagaacggc tcgcgctcgt
     8401 cggtggattg ctttcacact gcgcaatcga cttggcaatg catccgcttg tcaactactg
     8461 cgcccgtcgc gacacactgc gtcatggcgg ccacgaaagc acccaccatc gcctgaccga
     8521 aaaatatcag gcgctgttta tccatctgtg gcgacttggc gacgatcaga tcggcctgcc
     8581 tgcttttgcc gagcgctgcc gcatcaccaa acacggaaac tcgctgtcgc tgaaagttga
     8641 gcagccgatc cttgacctga tgcagcaggc attccgcggc gcctatggat cggccccgtc
     8701 ggtgcttcag tttagcaagt gggtccgcaa cttccgtcag tttggcatct tgctgtcgac
     8761 gccctgggtg gccaaaaaca gccagcgcac ccgcaccgac gcgattcgcg agcgctattt
     8821 tgtaaacgcc gagtttgacg cccgtgagtt tttcgctgcg gcagagcgtc gactcgggaa
     8881 aataagtacg ctttcttacg attatttcgt cgcaggtgac ttttccccgg cggcgcaggc
     8941 cagctacctt tcgcaggtcg gcattgatga cctggccgaa ccgcagtcgg tcgatctgcc
     9001 gcagattccc tatcttccgc cggtgcgcgg caaagtgcag cagcccaagc agcccaaaac
     9061 accgcgcatc cgtctgccgc aggtgcgtct gcgtcgcaaa aagaatcctg ccgcgaccgc
     9121 ttcgatgtaa actgcagccc ccccgtcgat tcgtcgtcat ttccgtcaca cattcatcca
     9181 caaaaagcgc agccaggggt caagtcctct cgggtgtttt tgtgcgatcg cgttcaagcg
     9241 ccgaaaaaca ttgagctaac aaacaatttt ttctaacgaa gacgtggtaa catgattgcc
     9301 gtgctatcgc gtaccttcac gctcgggcgc tatacctgcg tcgagaggct tggccaaagt
     9361 cctgtcggcg aggtatggcg agccaagcga tttggcctgg cgggtctcga ccggcagtac
     9421 ctggtctatc ggatcggggc gtcgcagatt gcccaggact cgaccgccaa tctccgtctg
     9481 caagcagcgc tgcgcagcgt gtctgaaatc cagcaccaga accttctgca catcgaggag
     9541 ttcggcagcc aaagcggcga tcagttcgtc gcctacgaat ttgttggttt tgccgatctg
     9601 gggaaactga agtccggaat cgacctgctt ggcgacgagg ccaagtcgct tctgccgcag
     9661 attgtggcgc ttatcggaca gcagcttgcg cgggcacttt tggcggcgca ggaacgcggc
     9721 gtcatgcacg gcatgctgtc gccgtcgtgc atttggattt caccgggagg cgacgcgcac
     9781 attgccgagt tgggactgtg ggcggtgctg ccgcaagggc cgtggaagct cgacggagcg
     9841 ctcaagtcgc ttggcccata tgttgcgccg gagctgctcg acagtggggt gccagggccg
     9901 cgcagcgatg tgtttgcgct gggcagtctg cttgcggaac agctcaagct ggtcagccaa
     9961 gacaagctgt cgcctgattc aaagtcggcc cagtcgtcgc tgcaaaccat ctgtcagcgc
    10021 gcgatggaaa agtcagccgg gcagcgtccg tcgagcatgc tggaacttat caagcagctc
    10081 gatgcggtgc gcgtgaccga agcggcgccg gctcagctcg cccgatttgg tcagctgttt
    10141 gagctggccg gtgacaccgt tccacagccg gtgacgacca gcgatcgctc ggcagccgag
    10201 ccgctgccgc cgccgccgag catgaagatc gccgtcggca agaccccggc caagccgccc
    10261 gaaaaagcag ccgccaaaga cgcggtcggt agcgaaaagg ctgcgccaca agacaaggcg
    10321 gacgccgcgg acggaaaggc cgccgccagc cccgtgacga taaacccacg ggccgcattt
    10381 ggagccggaa tggcaggcaa cggcgcgtct gcggtgacgc cgaccgccga tgtcgatgca
    10441 gccgaggcag agctcgcagg gggcgatgga aaaaacaaac cggcactgcc ggcccgcttg
    10501 cgcccaagca cgccgccgcc cgataccgat cacatctcgg tgacggatac caacccggcg
    10561 atgatgccgc ttccgaaggc aaaacggacg accgccaagc catcgaaagc cgtcggtgac
    10621 gagccaacca atcagtttaa gctagaggcc gccgaagccg cgctcgccgc cgagcaagcc
    10681 gccgcagcgg ccagcgcccc cgcagccgat ccgcccgtca gcgatccatc gacgctggaa
    10741 gcatcggagc tgctcgaaga aaatcctgcg ctcaactcga ccggcaacta ctcccagcca
    10801 gagctgccgc ccgtcacccc ggaaattgct gctgcagcgg gaatgagcca atctccgtcg
    10861 ggcagcccaa gcgccccggc tgtcgaacca tccccaccgc cggaaagtca gccaagacgg
    10921 ctgtcaccgg cggcatttgc gggcatcggt ctgggcgctg ccgtcgttgc aggcggaatc
    10981 atgttcgctt tgatcggtgg caattcgaca agcaattcgg taatcagcga cggtggtgcg
    11041 aaagacggca tgacatctac ccaagacgtc gtcgccaagc tgccgagcga cgaaatccag
    11101 gtggacagcg cgccatctgc tgcggtcttt ctcgacggag aatcgaaggg aaaagcgccg
    11161 ctcaagctga aggtcaaggc cgggacccac aagctgctca tcgtcgccga ttcgttcaag
    11221 ctatggaaac aagaggccag cggcggtcag accatcagcg ccaagctgat caaagccagc
    11281 cttcccgacg acgtaagcgg caccgcggca gtcaaagtca agtgcaagcg cgatggtgag
    11341 ctgcgaatcc tcgtcgacgg aaatgacagc ggtctaagct gcccgacgga cgatctgatg
    11401 ctcaaaccgg gcaaatacac gctcggcttt ttgagcccga cttccgacga gctgcgcgaa
    11461 aaatcgatca aggtaaagaa gaagggcctg aagctgaaag tgaagttcta gcccgcccag
    11521 gcagcccgcg cgcccaggca ggccagcacc cgcagctact cgacgtcggc gtctttgggc
    11581 atctgacgat tcggacccac aggtgccagc ttgcgcaccg ccagctcgac aagccgcgcc
    11641 gcttcccggt tttgttcggt ccgcggcact ttcgcgaccg ctttttgcaa ctgttcaaag
    11701 cggagcaccg gcccaatgtg tgcggcgacg ggagtgcctt gcttgggcag cacgttgcct
    11761 tttggcatcg cgtcatgtgt accttccagg tacatcggca gcacgtcgat ctgatggtgc
    11821 agcgccaggt agccaatcga cgccttgaac gactgcataa tccccgattc cgaccgcgtt
    11881 ccctcgggga aaatcagcag cacatagccg tcttcgatga ctcgtgccgc cagccgcaga
    11941 gactctcgca gcgaacccgt gcgatccatc gggatcaggt tggtgaagtt ttcaaagtag
    12001 acgcgcttga gcggatcgtc gaagaagtag tctttcgccg cgagcgccac cagccggtcg
    12061 ccccagtcgc cgagcgcatg cttcacaagc cccatgtcca ggtggctgct gtggttcgca
    12121 gcgacgataa actgtcgcga agctggcagg tatgctttgc ccgtaacccg cgtttttaac
    12181 acccgctcat aaagcgcccg ctggcccgcg ttgagtccgc ggttgcccgc ccgcaccagc
    12241 caatcgggaa gcaccagcct gtcgtcggtg ctttcgtttt tgtccttctt tttggtcttt
    12301 tccttgggcg cctctttgac cggcttgcga cgaccccact gggcaatcgc tttctgaagc
    12361 tcggggacgc tgacgatgct ggtgatgtcg acgttttctg gcacctgcac gcccgccgct
    12421 tcaagcgcaa cacccagctc ggcaaacgac agcgaatcga gcccgagttc ctgcatcgtc
    12481 gattccggac gcaccgcaga gcgcggcttg cccgtcacat ccgcgagcag cgaaagcagc
    12541 cagtctcccg ccgtcgctgt accgccgctt tggacttcgg cctgcgcctg tcgccgcgtc
    12601 cgctcgagcc gtcgcagctc gtcgaccacc tgcttgcgct tgaccttgcg cgtcgccgtt
    12661 ttcggcagct ccagatcggt caggtgcaaa atcttgactc gcttggccac cgacagcttc
    12721 gccgagacat tgcggacatg ctcgcgcact ttttcgcgga cggcttcgcg cgaaagtcct
    12781 tccgactgcg gcgcgtcgta ggccgggacg ataagacagg ccaccgcttc gcctttgtcg
    12841 tcgggcgatg gattttcttt cagctcggct tcggccagac cgacgacgca cagctctttg
    12901 acaaacggcg actcgcgata gcgttcttcc agttcgtcgg gatagacgtt ttcgccgttg
    12961 gcaccgatga tcacgtcttt cttgcgtccg acgataaaca ggtggccttt gccatcgatt
    13021 cggcccaaat cgccggtgtg cagatagccg tttttgatcg tctctttggt cagctcgggg
    13081 tcgttgtaat accccagcat cacgttcgga cccgacgcga tgatttcgcc cgtccccgtc
    13141 gcatccggct cgtgaatctt gacgtcgaca ccgggcaggg cttcaccgac gctgccaagc
    13201 agaagcggcg aacccggcct tgtcacggtc agcaccggcg cgctctcggt catgccgtag
    13261 ccttcgtaca ggttaaagcc gagcccgcgg aactctttca tcgtctcgat gggcagcgcc
    13321 gaaccaccgc ttatcagaag ccgcagcttg ccgccgaacc gctggtgcac cggcaaaaac
    13381 agtgcccgcg gcaagttcag ctcgatgccc agcgccttgc gggtccgttc gcgcgccgac
    13441 cgcgtcaggt cgtacagccg ctccaaaaga cgcagcaccc atggcaccgc gcgctgcggc
    13501 agtcgatccg aaatctgctt ggtgatgcgc cgatacaaga gctgatacag cgccggcacg
    13561 ccgaccattc cggtgatatg cgccgacgaa aaagcgttct gcagcgagtc agcgttcacc
    13621 tcgggcagat aggcaatctg cgccccgcgt gacatcggca tcagcagacc ggcggaaaat
    13681 tcgaacgtgt ggtgcagcgg cagcaccgac agaagaccat cgtgcttgtc gacatcgaag
    13741 acgccgccca ttttggccaa aagcgacgtg aagttgcgat gcgacagcat gacgcccttg
    13801 ggccgtccgg tcgtgcccga cgtgaaaatc agcgatgcca tctcgtcggc gcggcccgac
    13861 agcgtaagcg ccggcagact gcttgccgcc acaagcgcgt gcggcgtttc ggcagagaaa
    13921 agctcgcgga aattgagcac tttgacgctt ggcatcgtct gcgcaagcgc gccccgcacg
    13981 tcgatccgcg cctgcacttt gtctgacaca atcgccgcgt agacatcggc aaaacgacag
    14041 atgttcagca cttcttcgag tgaggcgtcc ttgtcgacgg gaaccaccac ggcggcgcat
    14101 ttcaaaatgc caaagtacga aatcgcccaa tcgggacggt tttccgacag cagcagcact
    14161 ttgccagaca ccggcagccc gagtcgccgc agcgtctcgc tgacctttgc cgagcgctcg
    14221 ctcagatcgc gataggtgta gcgctggccg tgcccaatcg gttcgccgtc ggcatcaagc
    14281 ggcggcagaa gtcgcatcgc cgtccgaccg cggtaggcct tcgctgcgga ctcgagcatt
    14341 tcgagcaagt cgcggtaggt gtagaccgct ttggcgcggg cttgaaattc ttcatccagc
    14401 gacgggaaaa tccacttttc cagacccgga atgtggacgt ccagcatgta ctcgcgccag
    14461 ttgatcgttt ccggattcca gcgcaaaagc gtctggtcat gtggcgtcag actgcggaac
    14521 agccgtcggg tgttgtcggc gcggaaaatc ggcgcgttat cgtgaacaaa cgggaaaaac
    14581 aggtcaaaaa tcccttcggc ctgcttcgcg agctgcgccg tttcgtcgag cttttcgcgc
    14641 gcccgttcgc ccagtgccgt cagccgcggc atcccccaca tcttgtcgag tgcatcgccg
    14701 atcatgcccg acgccgcttc ggccacccca cgcagaagcg gcacgctgag cttttgatat
    14761 cgggttttgc tgacgggaat cgactccagc cgcgatcgca gccggttcat caccgcgtcg
    14821 ccctgcttgt gttcgagctt ttcgcggaag tgtcgccgct tgtaaagacc ggttagctcg
    14881 acggcacgcg gcatccgcag cgggctcgag tcgcttgaac cgaggtgata gatcagttca
    14941 ttgcgctcgg ccagcgtcgc tgccgtggct gcaatcgtcg ccgacgccac catgtcgacg
    15001 ggaatcatgt ccagcaccgt ccccggcgaa gcgggaactt gaatgtggcc ttttaacacc
    15061 atgaaaatca gcggtgcggt ggtgttaaag ccttcgttcc agccggggaa cggatagctc
    15121 atcgccgatt cgacgatggc cggacggaca atcgcgacgc ggaccggctt tttgccgtct
    15181 ttgcgctcgg attctggcag cgtcgccatc gcacagagct ggtcgccgat gctctttgtg
    15241 taggtgtacg tgttggtgaa gccccagtgc tgcgcgcgct ccatgcccag ttcggtgagc
    15301 cgctcggcgg tccaggtctt tttttcgcgc tgcagcgctg ctttcaggtg ccgctcgtcg
    15361 tcgggatcgc ggccttcttg gcgcagcctg tcggccccct gttcgcggaa catcgacagg
    15421 tgggcgcggt cctcggaacg ctgacgggtc tgttcaatca agcgttcgca gtcggcaatc
    15481 tctgcttcgg gatcgaaatc gcctttgcgc agcgccgatc ctgccgacaa cgagcgatcg
    15541 tgaccgggac ggcgagggaa atagccaatc agtgactcgt cttcccagac ttcgccttcg
    15601 cggcgaccag cgacgaaaca ggtcgagacg tgcacgactt tggccgatgt cgcccgcgcc
    15661 agatcgagca catagcgaac gccgtggacg ttgatgcgca gcgccgattc cagcgacgga
    15721 ttgaacgaca ccagacccgc cgagttcaag atcacatcga gcggcccgtc ttgcgtcaaa
    15781 agcgccagat cggcgtcgga aagaccgaca ttcggacgcg aaacgtcacc cgccagcggc
    15841 accacttttt cgcgcagata tttttctgtg ccgtcgccaa acgtcttcca gaccggatca
    15901 aagggacgcg acttggcaat tttttggaaa aaccgtgtct cggcggtgta gcccgatccg
    15961 ggacgaacca gcgcatacag cttgccgagc atcggatact tgcacagcag catcgacatc
    16021 gtgactttgc cgacgaagcc ggtgccgcca ataaacagaa cgcgcttgcc ttcgagcgtc
    16081 tgggcaacgt caagaggagg agcggacggg gtcatggtcg gacgaagcta ctacgaacga
    16141 cgcgaaacgg gaagcaagca gcccgaaagt tgcggcggtt gtctggtcga ttccgcaaag
    16201 ctgactatga tgcaccgacc accgaaccaa accgcggcga ggactcccga tgaaatccgc
    16261 ttgctctctt ctttcgcaat ctgctgcacg ctcgcatcgc ttcctgttgg ggacgttcgt
    16321 atttgcgctg tgtagctgca cgcatgggaa tcaaggaatc gagctgggag gcaacattcc
    16381 accgtctgtg ggaacggaga ttttgggcct gccgccgatt gatccaaacc tgcagtacaa
    16441 ccacaccttt gtgcagcaga cagaagccga ttttttcgga tggagcaaaa acggcatcgt
    16501 cgcaagtcgc gatgggaatc gggtacggct tgtcctttcg cccgaaaaac agggaacgct
    16561 gcgctgcgaa agcgtcgaca tcgacggtgg gatggcgcgc tttgatgccg caagcgggct
    16621 gtgtgcaggg accgatccga tgccgggcgg gctcccaagc ggtgtgaact actacagtgg
    16681 cggatcgttt tttttcggca cgatgacatc gccggaagtg acgccgcgag cgccgattga
    16741 tcacctgatt gcgtcgtgga atgcgcagac gccaccggga acctggctgc agctgcgggt
    16801 gcggatgaaa gtcggcggtg cgtggagccg ctattacaag ctccccattt ggagcagcga
    16861 tgcagcagtg gtgaagcggc acagcataaa ggaagccgcg gacgataccg gctatgtcga
    16921 taccgatacc tttgagctgc gcaatcaaaa gacggcgacg gcgtatcagc tcagcgtgac
    16981 gctgttttcc accgatgcgg ccaaaacgcc ggcattgggt ttggtcgcgg cggtggcttc
    17041 gcgcgacaag acggactatc ccaaacaaga cctcgatccg tcggtgtttg ggaaaaacct
    17101 gccggttccg cagcgctcgc aagagcttcc ggaatacaaa aaaccggaat accagcagta
    17161 cggcggcggc ggcgaagtct ggtgcagccc aacctcgacg agcatggtga tggagtacta
    17221 cagccaaaag ctcggcgatc cgcagctaaa ccagacagtg cccgacgctg cgatcggctg
    17281 ttatgactgg gtgtacaaag gcaccggcaa ctggccgttt aacaccgcgt atgcgagcac
    17341 gctgggactt gccggttacg tcacgcgctt ttattcgttt gcgcatgcgg cgccgtttat
    17401 caaagccggg gtgccgctca tcatcagcat cgcgttcaaa gcgggcgagc tgcccggtgc
    17461 gccgatcagc aagaccaacg gccacctgat cgtcgtgcgc ggcttcgaca aaaacggcga
    17521 tgtgatcgca aatgacccgg cggctcgcga caatgcgtcg gtgcagcttg tctatccgcg
    17581 ggcagcgctc gaagcggcgt gggcccactc gcatcgcacg gcgtatctgc tgtatccccc
    17641 gacgggtttc gatctgccgc agcttccgtg acggcgccca agtccggcgc tacgaatgcc
    17701 agccaggtgg acagtgcgca gccggaacga agttgccgtg aaaaccgggc ggaatgtgat
    17761 gtggcagctc ggccacgcag accgtcgaca ggtcgcgcgc atccagcaca tagagcgccg
    17821 atcgatgctc actgccgtga tacacgacgg tgagcagcca accatcgtct tcatcctgcg
    17881 cagacggacg cggcaccatc accggctccc ccggaattcc cggcgcaaag tcgcggagct
    17941 gcatgtcgcc ggtttggaca tcgaagcgcg caatcgccga aaacagcccg tgcggcgcat
    18001 ccggcatcgc tgacaccgta aacgcatagc gatagggcag gccttgccgg gccggatgaa
    18061 tccgcggcag ctcggacgaa aaaccaccca gggggctctc gctgacggcg gttccttctt
    18121 cggcgcttgg gtcgatcacg tagcgcgtca gcttcacatg cgggatgtcc gaaatgtgac
    18181 ctgccaacag ctcgcccggt cgcggcgcgt caggaaagtg atcctggcga caggcgtcga
    18241 cgaccagcgc tccgttttcg tcctcgtaag cgttcacaaa atggagcaca aaacacggcg
    18301 acgcttggaa ctttttcact ggcccgccat cgcgcggaat cagcaaaatc gtcgtcattc
    18361 cgccttcgac ttcttgcacc gatcccgacg gcggacggcc catcagcgtc ggcaacatac
    18421 gcagcgacac cggaaacaga aacagcacct gccagtttgg cgtcagcaaa aagtcatgcg
    18481 cgagcagcgg cgccggcaaa ggaatttccc gcgtcaccgt cagcgccacc ccaccgcgaa
    18541 tgttgtagag caggatcttg ggatgcggcc caaggtacag gccaaagttc cacagctcgc
    18601 cagtgttggg gtcttccttg gggtgggcgg aaaacggcag ctcgggcaga agcacgcgct
    18661 caagcaaggg tccgcggttt ttcagcgcac caaagaagtc gtagcgctca atcgtgtcga
    18721 ggttcaccgg gtccagcagg tgcggcaagc cgccttccca cagcgcaaac agcctgccgt
    18781 ggtggtagat gacgctggtg ttggcgacgt tttttacgtg ggtgcgaaac aggttgccga
    18841 aaaatccgcc ggcgcggttt gtgccaaagt tgcgatacag aagtcggccc gatcgctgct
    18901 cgtacacaaa ttcggtggtg cggacgtagc tgttgcgata gcgaatgcac ggctggccgt
    18961 ctggaccgtg gctgccgatg gcgaagcgaa tcaccatgcc atcgccgtca atcaggtgtc
    19021 cataggtctg gccgtggttg gcaaatcggc ccggtccgtt gcgaaacaaa acgccgcgca
    19081 agtccgaagg cagccgtccc tcgctgatcg gcaggaccgc ctgcacttcc tgcgtcacgg
    19141 gctggtgcgc agcgacatag cttttgacaa tcgcgtgctg ggtttccatg cgtcagccta
    19201 tgaccagttc cgcttgcaag agcttgctac gatcccagcc acggcagacg gcctgctgtc
    19261 aagccagccg cccaagtccc cgcaatggct cacgtttttg aaaatgaaac gaagcgcgcg
    19321 cacgcggtca gactaagacc tgcgatgtga gaagcaagtg atcttgtacg gggaagtgtg
    19381 caatccgctc atgtgtcgga ccacttgacc gcagggtggc acggaaacgg ctcagccgcg
    19441 cggcgctttc ggcgatgcgg tcgcggacga tgctgctctt ttcagcagcc cggctgagtg
    19501 cctcttcggc gcgcaggagc cgttccgcat cttggcagaa caaaaacgca tcgcagccag
    19561 ccagcaggct ctcaaccacc accccgctgt catcggcaat tccgagctgc tgcgccgtca
    19621 ccgccttcat gtccagatcg tccgacacaa cgacgccgga aaagcccagc tcgccgcgca
    19681 gcatgtctgt cagccatttg cgcgacagcg tggcgggccg cgcatcgacg gcgggataga
    19741 caacgtgcgc ggtcatcagc atcccaatgt ccgccgccga agcccgctca aacggcaaca
    19801 gctcgacctg ccgcagcgtc gccagatcgc gcttcaccac aggcagcgtc aggtgcgagt
    19861 cggtttcggt gtcgccatga cccggaaagt gcttgccgca gccacgcaga ccgacgttcc
    19921 gctccagccc gcgcagatag gcaagtgccc gcgaggccgc agcttccggt agcgtgccaa
    19981 acgcccggtc gccgatgatc ggattggccg gattggtatg cacgtcgagc accggcgcaa
    20041 agctcacgtt gaagccgagc gccgccagct cccagcccat ggcctgaccg accgcttcgc
    20101 aaagctgctc gggcttgtcc gcaagcttgt gcatcggcgg ccacaccgtg acgtcgccgc
    20161 gcagccgctg cacccgtccg ccttcgtgat cgatcgccaa aatcggcgcc gcctgttcca
    20221 ccgcagacga cggagcatag ccacgcagca cggactccat gtcgattgca atcgcacgcg
    20281 cgctatcggg attttggaag ttgcgtcgaa agaaaatcag cccggcaaag tcgccgcgac
    20341 gaaagcgcgt cgccagcgaa gtaggcagct cggtgccctg atatccgacg ataaacagct
    20401 cagcaggact tggcatcgtc cgctccttgc gaaagaagat aacccacaac ggccagcgca
    20461 gcgacgacag cggtttcggc ccgcaagacc aaaggaccga ggctgaccga cgaaaaaccg
    20521 acggctttcg cggcttcgat ttccgaatca gccaagcccc cttctggtcc aatcagaagg
    20581 ctcaccacct ttggcgcagc ccccggatca agcacctgcg caagcggtgg tgcgcagcct
    20641 tcgtaaaaca gcagcttctg tccttcggct tcccgcagtg acacttcgag tgtctgtacc
    20701 tgcgcaatgt cgggaacgtc ggcgcgcttg gactgctggg ccgccccttt tgcgatgtct
    20761 tgccagcgac ggtgttttgc agctgaccgc tcaccgagaa gctgcggcac gctcctatca
    20821 cactgcacag ggacgatgcg gcttgccccc agctcggtcg ctttttggat cacgaagtcc
    20881 tgcttgtcac ctttgagcaa cccgacgaaa agcaccagag tgggctgcgc gaccgacaca
    20941 tgcgtcgggg gctccgccga tagctcgatc gtctgggcgt ggaccgcaga caccacagcc
    21001 agcgcttgcc ggccctgtcc atcgagcagc gtcacctggg cccccggtcg cagccgcagc
    21061 accgacgcca ggtagtgatg ttccgcgccg cgcaacacca cacttggccc cagccggtcc
    21121 ggagaaacaa acagccgcgc ccgcgtcgcc acaaggcccc ccagaagcga ctagcggtcc
    21181 gatgccatgc gccagcttgc gccgtaccaa gacgcctgcg caccgacggg ttctagcgga
    21241 ataaagacgc cccacaggag ccgcagcgtg cgacggatgc catcgcgtga gtaaccaacc
    21301 agacctgaca ggatgctcca ttccagatcg cccgcacgcg gacgtccgac gtggaacagc
    21361 ggccagaact cgaagcgata cgcgccggcc cgctcgcccg tgcccttgta gtagtagctg
    21421 ttgagcacca gcgtgtagtg gcccttttcg ttggcccagt gcgcgccgat cgggaagaac
    21481 accgtcgtcg tctgcttggg tcgcttaaag tgccaaacaa acgggaagac cacgcgatca
    21541 cggctgccgt cttcgtagtt gcgaacccag gtgagcagcg gcggcaaaaa cagcgtcttt
    21601 gtgccttgtt catcgcgacg atacgcaaag aatggcagca gcgccgtgaa ctgactggcc
    21661 ggacgacgca catggtagaa cagcgggaac agcaccgtcg tcgagcgatc cttgccaccg
    21721 aagtgccaca caagcgggaa caccaccgcg ttgtgatagc cgtcagcacg ctttttcacg
    21781 taagtgagca gcggcgggaa cacccaagtc gtcgtcttgg ccgcatagtc ggaaagacga
    21841 accacaagcg gcacaagtgg acccaccgcc gtcagccggt cgctgtgcag gtgatgcaca
    21901 tcgagcacga aggggaagaa cgtcgtgatc cggcgcgtca gtgtccgctc atgccaaaac
    21961 agcggtgtcg ccatcgtcaa gctacgctcc ggcgagcgag tctggagcca caacggcagc
    22021 agcatgctcg tcgaggtgtg cgctttgtcg tcgcgggtgt aatagaacag cgggaacagc
    22081 gccgccgacg tcaccggatc ttgcttccgc acatacagcg gcccaatcca gaaccggaaa
    22141 ccaaccggat tgcgattcac accgacgagc ggcgtaatca accagtcttc tggggccttg
    22201 ccggcacggt gcgaaaagta aaccagcggc aacgccatga agcgatacga gccgtcgcca
    22261 tcgttgcgac cgtagaacag cggtgcaaag ccaccgaacg tcgcttcgcc gcgccgaccg
    22321 aaatacagcg gtccaagcac ggtcaggtgc cgactgcgcg ccgcgtcatg cgagtgataa
    22381 atgaacggca gaatgaacag tccatgcgcc gtgtcccgtc gcgagtagaa ccacagcggg
    22441 aacacaccga agtcttcgcg gtccttgcgc acgtcggcgt aaaacaaaag tgccgccgcc
    22501 cgcgtcgctt tttcgtcttt gtagaagaag ccaagcggcg tcgtcgccca ggtggcttcc
    22561 ttcgacttgc caaacgcgaa cagcggcaaa agacctgccg acaccccgcc cgtcttctca
    22621 ttttttgaat agaagaacgg caaaagcacg gtcgaggtcc gctccggact ccgcacatgg
    22681 aaaaacagcg gcagaaggac atgcgccgtc cgctcggggg tcttgacgcc gtaatagagc
    22741 agcgcgatct ggcggaactc ggtgtccttg tccttcacgt ccgcaaacag cgggaacagc
    22801 accgatgccc gtcgacccgg tgagcagtgg aagaaataca gcggcagcac cgcgcgattg
    22861 atgacctgct cacgggtgcc aagacctcgc accgtctcga catacggacc gacccatacg
    22921 gtcttgcttt ccggcgtgcg gtgataggca aagagcggcg caatcgcaaa ggtcgacttc
    22981 ggtccttgcg aggcatagaa cagcggcaca aggccggtcg tcttgacctc gccttcgggg
    23041 cgcttttcgg tggtgtggaa gaacggccca atcaccagcg ccgagccacg cggattccgc
    23101 acctgccaca aaagcgggaa cagcacggtg tagctgctct cgggcgagcg cttccagaac
    23161 agaagcggca caagtccggc ccgcacttcg gcgcgcttgt caccgatgta ttcggggcac
    23221 ttgcgatacc agtaaaacgg tccaagctgg agcgtttctt cgtgcggacg acggtgatgg
    23281 tagagcagcg gaaacagcat ctgggtgctg ctctgtccgc gacggtggag ccacagaagc
    23341 gggaacaccc cagcggtata gccggcgtcc tcgccgcgcg tcttcacccg tgcgaacacc
    23401 ggaccgaccg atacgtagct cgaaccggcc tctttgtcgg cggcataaat gaacagcggc
    23461 ggcaaaatcg caacgtgcgg tttgtcaccc cgcgcaaccg aaatgagcgg cagaagaccg
    23521 ccacccgagc ccgttttcga cgaccagccg tagaccggtc caagaaaacc aaacttgcgc
    23581 gtcggcgagg tatgaagcgc ggccaacgcc ggcacaaaca catgcgtgtc gctctgcgcc
    23641 cgcttgctaa agaagtgcag gtacagaggg agaagacctt cgctgccacc ttcttcgtcg
    23701 cgggtaaaga agaacggacc ggtataaccc caggccgcgc ccgtcgtctt gttgcgatac
    23761 cacatcgcca gcggcggcag caccgtcgta aagcgccgca ccggatcatc gcgatggaac
    23821 gtcggcgggg tcaaaagcac cgtcgtcgtc gattcttctt ccggattgcg atacgaaccg
    23881 ccgagcggcg tcaccaaaag ccggctcttg tcggcgccgc ggtgatacag gtacagcgga
    23941 aacagcgtcg aagtccgcgt acccgtctgg tagttttcgc cgtcatagaa cagcggcagc
    24001 accatcgtgt ggcgcttttc cgccgtcttc gagtgaaaga ccagcggcag gaagcccccg
    24061 acggtgcgat ctggcgtctt gcggtaaaaa ccgagcgcca aaaagaacgc ctgcgacagc
    24121 tctggatctt tgcggacaaa cagaagcagc ggcaaaaacc cggtgtcagt ccgcttttct
    24181 tcgacgttgt ggtgatgcca aaacagaagc ggcggcaaca ccaccgtctg tgcgtcggtc
    24241 cgtcgcgtca cccagaacag cggcaaaagt ccaacggatt tgcccttttc gccgctgtcg
    24301 ccgtagctct gatagaaaaa aatcggccag atgcggtgcg cccgcatccc cggttcgcgc
    24361 ttgtactggt acgtcggaaa gacccaggtg tcgcgggtct tttctttgtc atccttgcgg
    24421 tcggcaaaca gcgggaacaa gaccttcgtc gtgcgatcgg gccgccagaa gctgtaataa
    24481 aacggcagca ccagccggta gccgatcggc cccttgcggt ggaagtacag aatcgccgac
    24541 aagggccggt tgacgctctg gcagctctgc ttgatacaga ccgtcttgtc ggggcactgc
    24601 gcgtcgctgt cacactctgg cttggccttt ttggcttctt cgcgtttgcg ctttttttct
    24661 tcttcggact cttcgtcctc ttcgtcgacc acttgcccag gcgacggcgt cgcgaaacgc
    24721 tgcggatctt ccgggtcagg cgtcgctgtc gtcgtcggtt tgttctgcgc ttgcgccaac
    24781 agcggcagcg gcagcggcag cggcgaaatc cccggtggaa ttgccgtcga cgcagccggg
    24841 atcggcggag cgaccgttgt cgactgattt gggccagcca acgacggcag cggtgacagc
    24901 gccactttcg caagcggtgc cgccacgccc tttggcagaa gcgaaactcc cacaggtcgc
    24961 ggcgcagcca tggcagatcc ggtccccgca aacagcaggg ccgacacaat cgccagacgc
    25021 accgagaggc cgcggagaga agttacggcg tcatcttcca ctcgccgttc tccagcacaa
    25081 ggttcacttc gtcggtctgg acgttttcgc ccttgctacg gcggactttc agcgtcgcca
    25141 gcttgaactc gccctgcgcc ttgaaggtgc tgccacgcag ctcaaactgc acgccctcta
    25201 agtctttttg cgccttcttg agctcgtcgg gcgactgcat catctgctga ccgacttgcc
    25261 cctgatacag catcgcgtag cagtcgatcg ctttttgcac gtcgcctttg gccatcgccc
    25321 cgcgccagcg ctcaaacgtc ttttccggcg tcgaaaactc cgcccgcggc ggtggcagct
    25381 tgaaggtgat cgacttcacc ttgtcgcgcg gcagctcaag cttttgcccg ccgctcgtct
    25441 ccatcacgat gatgccgtcg aagtaatggg tgagcttgcc tgcgatctgg gtgttgtcgg
    25501 cgagagttac cacttcggat gcgtaggcac tcacagacga caagagcaac gctccgaagg
    25561 caaaacaagc agaacgaaga gcagatcccg ccacgcaaga catggcgttc cagccagaag
    25621 gcatcaggca aactcctatg ggtgaggggc tggatttacc ttgacttttt gctgctgtca
    25681 aggcgccaaa gccagcgttt ccgtcggtga aaccctagca aaaaagtagc gaaaaatcgc
    25741 cccaacaaac ccttttgatg agcggttagc tgcgcaaata gctgatcacg gcgtccgcac
    25801 tgcgcagacc atcgatcgcc gcgctgacaa tcccgccggc ataacctgcg ccttcaccga
    25861 cgggaaagag tccggcaagc gatggtgaca tcagcttgtc atcgcgaaca atgcgcagcg
    25921 gcgacgacgt gcgcgtttcg accccgacaa gctgggcctg cggcgataca aagcccggca
    25981 aggtacggcc aaaccgctgc accgcttgcc gcagcgaatc gacgacaaac ggcggcagca
    26041 catccgccaa gttcgcactg acgatgcccg gtcgataggt ggtcggcagc acatcactgc
    26101 tcggctttcc gacgagaaaa tcggtcagct tgtgagccgg cgcaacaaag cgaccgccac
    26161 cggccaaaaa ggcccgctcc tcgacggcgc gctgaaacca catcccggcc agcggatcgc
    26221 cggcgaagcg ttcaaacggg gcgacatcgc gatcttcgac ggacaccacc aatgcactgt
    26281 tggccagcgg tgagtcgcga cgtttcaggc tcatcccgtt tacgcacaga ccgccgggct
    26341 cggtcgcgca aggaaccacc cagccgcccg gacacatgca aaagctgtag acgcctcgct
    26401 caccgaccga cgctgccact tgataaaacg ccgccggcag cgccggatgt tccgccgccc
    26461 gtccgtactg aatgcgatcg atgagcggct gcggatgctc gacccgcgcc ccgatcgcaa
    26521 acggcttgcg ctcgacgagg atatcttgcc gcaccaagag ttcgtagatg ccgcgggcgc
    26581 tgtgtccgac ggccaaaacc accgcatcgg ccaggacaga aagccccgat gcgcagcgca
    26641 ccccgagcag ctttcccgac ggtgcgcgta gaagttcggt gactttttca ccaaagtgat
    26701 actgcacccc gcgtgccgtc agatcggcgc gcagctcgag caggatcttg ggcaagcggt
    26761 tgctaccgat gtgcggacgt gactcgacgc tgattcgagg atcggcacca tgctcgatca
    26821 gcgtcaggat ggtttcgcca atcgccgccc gatctttgct gcgggtgtac agcttgccgt
    26881 cgctaaatgt ccctgccccg ccctcgccaa agcagtagtt cgaatcgctc gacagctcgc
    26941 cgcgggtcag cttcgccagg tcatgtcgcc gcggctgcac cggctggcct tgttctatca
    27001 ggatcacgtc cgcgcccgct tcgcacagcc gcttcgcaca catggttccc gccggaccgc
    27061 tgccgacaac caccacgcgc tttttttccg tcagcgaagc cgggggcgaa agccgctgca
    27121 tgccgccttg caggaacggc cagctgcctt tgccgcccgg acggcgcaga tcaattcgag
    27181 aacacgccgg gggcagctcg gcgccttcgg gaaagacctc gatctgcatg cgatagccaa
    27241 tcgggaagcc tttgcgcgcg tcgagcgact tgcgcaaaag ccgggcccca ccaggccgcg
    27301 gaatgccagc ccgtcgtgca gcttcgagca tcgccaccgc ccacagcgcg gtctctggat
    27361 ccgcatgcac cgcttcgtct tcgtcgagct gaagctgctg gagcgggaca aatatctcaa
    27421 cttgcgtcaa gcaaacgccc tttcttatag cccgtctttg cgaagctcag cgacgatttt
    27481 tcgatacagc tctttgtgcg aaaaaccgct caccacgtcg gggaatagat ggcctatctg
    27541 cccgacttca tccaggtgat cggcgggcac gcagcccagg aacttgcccc atttcgccga
    27601 ctgcaccgtc accagcccgt cgttcacacg cgggaccagc gggttgtcgc tgtagatcgc
    27661 aaacacgcct tcgggcagcg cgagcagcgg atcaagcagg tcgatgcgcg acgagttgcc
    27721 ccacgcgccg ccggcacact cgctgtttgc aatccgtccc gccgatcgcc cagcgacgct
    27781 gtagtaacga accgctggat cgtcgggatt ggcggcattc catctttgca tccccggcgt
    27841 cgtaagctgc gcaagcgcat tttttaggtt cgggctgccc ggcgcatccg tcagccccga
    27901 caccgccttg aggatggcgt ttatcacgtc gtaggaaaaa ccgggcacca gtccgcttac
    27961 cacgtcggca atttcagtgc cgcgatgcgg cgtgctgacg gtgacgagtg acgccacttt
    28021 gtttccgtat tttttgccac ttatcagcgc tcgtccgtcg ataccgccct gcgagtggcc
    28081 gatcacgttg accttgcaag cgttgtacgt cttgagcgtg tcatcgacga ccgcagccag
    28141 cgcggcagcc cgcacatccg acgattcata cggtggaact ttggcttcga cgacagtttc
    28201 tccgcggctg cgcagatcgg cgccgacttg gaaaaagtag tttaggggac caatccgctc
    28261 aaaaccggca aagccgtgcg ccaggacgat tggatacggt gcgcctttgc aggacagcgg
    28321 ctgcgcaagc tgcgctggct gttctgaatc gggaagcgcc gttccatccc cacagcccag
    28381 ggccgccaaa gaaacgccca caagcagtgc gaaggcgcgc ccacgctgcc ggaatgtcgg
    28441 ctcatgttgc ttcgtttccg tcgacgactg tttttgcatg tggtcctcca tttcttccct
    28501 tggtgaaaaa gcgagccatt tttcgacgat ggtttgataa agtaaatgag cttcggcagt
    28561 ttgcctttgc aggcctcatg tcgcaggacc agaccgtaaa tgacccactg attggcacgg
    28621 tggtcgaccg tcgctacaaa gtgttaaagc gccttggcgt cggcggcatg ggcatcgtct
    28681 acaaggtcga gcactcgttt ttgaacaagt tcttcgccct caaggtgatg cgaccgacga
    28741 aagacgagac cgacagaaag cgcttcgagc aagaggcgcg cctggcatcg gtgattcgcc
    28801 accccaacgt cgtcgagatc agcgactttg gcgtcctgcc cacaaaccag ccgtattacg
    28861 tgatggagtt cctgagcggt cagaccgtcg gcgacgccat cttcgctgga tttatggaca
    28921 gccagcttgt ctgccacatc ggcgtccaga ttgcgcgcgg cctgcagtcc atccacaagc
    28981 agaacgtcgt ccaccgcgac ctaaagcccg acaacatatt tcttatcgac cccgacaaga
    29041 cgggcgaaaa ccaggacgaa aacagcgaga cgggggacag cgagcccgcg tttgtcaaaa
    29101 tcatggattt tggcatcgcc aagtcggtca acagccacct gaccggcacc ggaatgaccg
    29161 tcggcacgcc cgaatacatg tcccccgagc aggcccgcgg cgaaaccgtc gactggcgtt
    29221 cggatcagta ttcgctcggc tgcattttgt acgaaatgct gacccgcgag attcccttcg
    29281 acggcaaagg cgcgttcgag gtgatgtaca agcacctcaa tgcccagccc gtcccaccgc
    29341 gcaagcgccg gcccgagcga gccgacttca tttcgtctga gctggaagcg attgtgctca
    29401 aaatgctcgc gaagaagccc aaggaccgcc acccgagcat gcgcgtcgtc gaactggcgc
    29461 tgcgtgacga agcggcgcgg cttctgcttt cgggcgcggg cgacgacacc atggtcggca
    29521 gcaccccggt ccatgtgtca aacgactcga cgccgcttat ccgcacgctc gaagacaccc
    29581 gcaagaccgt accgtttatg cccgtcgcgg ccccgcccga gacgatggcc aaccgcccga
    29641 ccaaaaagat cgacatcaaa gaccaggcgg aaatgatgaa cctcatcggg cgcatcggcg
    29701 gaattgtcca ttcgccgcgc tcgctgccgc ctgcgtcggt ccagcctggt gtgttccagt
    29761 cgattcttgc ttggtttgcg cgcctttggc agcgacttgc gcgtctgtgg aagtagactt
    29821 ccgccatgac gtatggggag ctgtggtggc gcgcgttttg gatgacgcag gcgatcgaag
    29881 tgccgatcta tctgctcgtg ctgaccaagt tcccggcccg tccccgctgg cgtagcgggc
    29941 gcgaacctgt gaccgactgg ccgcacaagt ttttctgcgc gtttttggct tcggccatca
    30001 cccatccgtg ggtctggtac tttttcccgt atgtcatcga cgactactgg ccgatggtcg
    30061 cagtcgccga gacgttcgcc gtggtggtcg aagcgctgtg gtttcatttc tgcggtctgc
    30121 gtcgcgctct gctatggtcg cttatcgcaa acggcacgag cgctacgatt ggttttctac
    30181 ggcactgacg gcgaacacgc ccctttgcat ttggagacgc ccatgtccga ctggctctat
    30241 cgcaccccga ctgcgtttca cggcaaaaag ctccaccgac tcgggctcgc ctgtagctac
    30301 ggcatcgacg aagacggagt ccgcgccggc atggaccgcg gtgtgaactt ctttttgtgg
    30361 acgatgaaaa acaaggcgat gcgagcgccg ctcggcgatg cgttcaaaaa ccgccgcgat
    30421 caggttgccg tcatcggcta cgccacgctg ggctttttcg gctggggcgt ccgtcacagc
    30481 tgcgaatcgc tgctgaagga actcaagctc gactacctcg atgtctacct cctcggctgg
    30541 ctcggcgtca ccacggcgta caccgcggcc accgaataag aactgatcca cctgcgcgaa
    30601 agcggcaaag tccgcgccat cggctgctcg attcacaacc gcgagcgcgc cggcaagctc
    30661 gccgccgaat cgccgctcga ccttctgatg attcgctaca acgccgccca ccccggtgcc
    30721 gagcgcgaca tcttccccca cgtcaaagcg caaaagccca gcatcttggc ctacaccgcg
    30781 acccgctggg gcaagctctt aaagcgcccg agcggctacg acggccccgt gatgaccgcg
    30841 ggcgactgct accgcttcca gctctcgaat ccccacgtcg acatcgcgct caccgggccc
    30901 gccagtcgcg ccgagcttat ccaaaacctc gacgcgctgg acaaaggccc cctcaccccc
    30961 gaagaagacg cctggatgcg cagct
//
LOCUS       DAORGN010000035        30827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_551_length_30827_cov_6.085240, whole genome shotgun sequence.
ACCESSION   DAORGN010000035 DAORGN010000000
VERSION     DAORGN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 30827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30827
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_551_length_30827_cov_6.085240"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKL17_06160"
     CDS             complement(<1..662)
                     /locus_tag="PKL17_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44345.1"
                     /translation="MKKLHLLCLFALAVMLGPGAAAAKKKPTGIRESKFFKVDMDHSK
                     VVGPVDRVQAALSLDLTSVTTDQPQYWPNEKVYLKVMALGRPSADVTGKLAKRDGVSS
                     DITGKLDENGVLVLTVLDGEAKKLELGEYRIDVSVKGGKGKGQATFSVVEGTLGSLSF
                     AHDWKKVTRPDELEKINGAWFLGNAAGAGKRWGNGLSFKNELRMSNQPFSGEVTVHSR
                     CML"
     gene            complement(750..1997)
                     /locus_tag="PKL17_06165"
     CDS             complement(750..1997)
                     /locus_tag="PKL17_06165"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNK44346.1"
                     /translation="MTQHRKGPPPSDNPLPAWLFEKPEPAMEKPIIVQKYGGSSVADP
                     DRLRLVAKRVSDTVRQGNRVVVVVSAMGKTTDQLIALAKSVCTSPPRRELDMLMSSGE
                     RISMALLAMALSDLGHDAISFTGSQSGILTNDRHSGARIIEIRPVRILDELDRDRIVI
                     VAGFQGMSYRREVTTLGRGGSDTTAVALSAALSADCEIYSDVDGVYSADPRVVADARH
                     LPSIGTRQMQELADHGAKVLNAQAVEWAARAGIVIYARKTHGSAKQTLIRSDDEAPIE
                     RGRATAVTGTNQIVEIQSRHGAAILRVLGEHGVSLRHVGLCGEMLEVALTLEDLPDFG
                     RTLDELRTVDPDLVLRDDLGMVTAVGPGIGSDPVLLGRLLSQLRDSGFVPSRVTTSPL
                     RISVWLPKDVVGDATRLLHALLC"
     gene            2112..2185
                     /locus_tag="PKL17_06170"
     tRNA            2112..2185
                     /locus_tag="PKL17_06170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2146..2148,aa:Pro,seq:cgg)
     gene            complement(2275..3024)
                     /locus_tag="PKL17_06175"
     CDS             complement(2275..3024)
                     /locus_tag="PKL17_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNK44347.1"
                     /translation="MRVAAVQFKADRKDRQGSRDRLLALCAQAAERGATLVVCPEMAL
                     TGYLFTDAADVARVAEPAQGESFAALSSLAAHYGLTLVCGYPERAEDAPTPCFYNSAW
                     IIGPDGTLLSNYRKRLLYTADETWAKPGDTPYPQVRLPWGTLTAGICMDLNDDRFVEF
                     LYGARADVIAFCTNWLEEGLDLHSYWRFRLLLSRSVFVAANTYGSEVCRGHDATEFAG
                     QSAIFDSRGRTLAKAPPAGDAVISAEVRLSR"
     gene            complement(3031..4617)
                     /locus_tag="PKL17_06180"
     CDS             complement(3031..4617)
                     /locus_tag="PKL17_06180"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNK44348.1"
                     /translation="MVEPRIVVVDDNQRFCEQVVAAFAEVGILAQGFTRGSEALPVIQ
                     SQQPALLVVDLLRQGSEGRWLLSQLFSPEAAVPCPSVLALVGVTDDLSVLPDGVDALV
                     RPVFPRQVVASAQRLLPIQTVRGGRQATLRGLPAVVDASAVHTVPASPMSPASGQAVP
                     RPVEDKVTARQPQAEKSPFEEDSDFEPGETLLAPESIQALAREMMRADPDDPALLQSS
                     DLLPIEDDVMRGDEDTQPREDQVRFGRTASAGPASAGGKVLAGDLTALPLFEVVDLIA
                     RQKLSGVLSVTATGRALSVYFLDGWIAQATAQGLPSLRLGRFLLELDGQLRQPEIDAV
                     ASQSLGRPGPSERDATRVATDGMMAAVGQGPESLLGQRLVRAGLLRRDELTQALSRQS
                     YELICEGLRMPSGQFEFERTRQLPASVLSEDLGGALALDPAQVLLEGLRRLDDWRRFE
                     QDVAEGAIYVSYAASSSELLRLGLSQAELSVLSLCNGRATVAEIARESRLPLSEVSRT
                     LGRLLTLRLLRRRLPALLAL"
     gene            complement(4733..5419)
                     /locus_tag="PKL17_06185"
     CDS             complement(4733..5419)
                     /locus_tag="PKL17_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44349.1"
                     /translation="MKFSRSLARLFAFTATMTTLGMSAQSDAHAILTSPKPRDTSDAN
                     KAGPCGPAPIVNGPNLYTPGATMTVSWKETINHPGCFVVEWSTDGNKTFTNLATVKHT
                     TVGALPRPYSTQVTLPAGNCTSCTLRMIQWMLADDLQACPPATIPLNSQYFSCAEMTT
                     DPSRFGDMAGMTVTDMAMSSSPDMAVTTPPPGDGGTTATGCNFPAQTATSTGGLLILA
                     AAAGLLRRRR"
     gene            5548..6165
                     /locus_tag="PKL17_06190"
     CDS             5548..6165
                     /locus_tag="PKL17_06190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNK44350.1"
                     /translation="MQETQAAMAVFVHPSADVSEKAQLADGVRVWNQAQVREHAVIGQ
                     GTQIGKDVYVDAGVTVGAHCKIQNGVYLYHGVTVEDGVFLGPRATTTNDKTPRAIFPD
                     GRLRGASDFSVQPTRICYGAAIGAGAVLVCGITIGKFAMVAAGSVVTRDVPAYGLCLG
                     NPARLVGVVCACGVKQSASDSFCPACGAPIPDEAKSLWTQLSPGR"
     gene            complement(6149..6475)
                     /locus_tag="PKL17_06195"
     CDS             complement(6149..6475)
                     /locus_tag="PKL17_06195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK44351.1"
                     /translation="MTHATYRPRFEVTARLENPSTDEPHQVIGLSRSTVSFACPEPPG
                     IGTEVDVVIHLPALRSSLHARGQVAWRNRQEPADLGVKLDEVDAAGRELHARYLQLVL
                     AASAQG"
     gene            complement(6661..7881)
                     /locus_tag="PKL17_06200"
     CDS             complement(6661..7881)
                     /locus_tag="PKL17_06200"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK44352.1"
                     /translation="MLSALYLFLSLILLFLAVGVVRPRWLSQTIGLVASVPLLLVSLF
                     VPQIVFAGLLTMLLFAHFGAFSTTVGLVGLLIHILSWGLLIRYLWQLRSVLPVVDGQP
                     IVDAEHPFPDHAEEGQDLEDGRAPSLWPSLFLRTPSIGKVDVIRDVVYRQVDGVRLRL
                     DVYRPKNRPAELLPSIVFVHGGAWVVGSKRQSPFLMFDLAAAGYVVFAVSYRLAPRFP
                     LPAAIHDCKAAVAWVREHGPEYGGTPFAIAMGNSAGGHLAAMLACSGSDRSLQPGFED
                     KDCRVRGGVILYGLTDFTGIFEHHPHPIAHYLLEELVFRRRYREDADVFRRAQPVSYL
                     SADIPPLLLIHGEHDVMIPIVEAHNFYQRLKEAGARRVHLCEVPMAPHAFEIAPTPLQ
                     QRTQRIIERFLATL"
     gene            complement(7910..8134)
                     /locus_tag="PKL17_06205"
     CDS             complement(7910..8134)
                     /locus_tag="PKL17_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNK44353.1"
                     /translation="MLRCADGTIYTGCAISLPKRLLAHARGSAKYTRSRLPVELLYAE
                     SVADRRAALRREAAIKRLTRSQKLALCGMT"
     gene            8151..8630
                     /locus_tag="PKL17_06210"
     CDS             8151..8630
                     /locus_tag="PKL17_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44354.1"
                     /translation="MIADGSTEALGGGRRAVSIACIDVRTGGMLACLAQGMRVDTGRK
                     LFAGVRVDGRMREQLDKCPQRDRQYFESADGKYLQVLRSGKDSFIGKVLEPATEARSL
                     EDIRRNVWSLLQRVCPGRRDEGEIRLYALESEEPTMLHGGLPQPGNPYSNEEDDDFR"
     gene            complement(8722..9102)
                     /locus_tag="PKL17_06215"
     CDS             complement(8722..9102)
                     /locus_tag="PKL17_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNK44355.1"
                     /translation="MPVTVAVNEPETVVHKGSMGLAKTELDVCKTPTPGGPVPIPYPN
                     VAMSSDTTSGSTTVKCDGNSIMLKNSSFGLSTGDEAGSIGGIKSNVTKGQADPVNYSF
                     DVKVEGQNVVRKSDPMMQNKKNTI"
     gene            complement(9087..10160)
                     /locus_tag="PKL17_06220"
     CDS             complement(9087..10160)
                     /locus_tag="PKL17_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNK44356.1"
                     /translation="MMVLHSTPFVGALLSFLDKDGSEGRTALLKATFEIRSDGLLQIA
                     EEQEPICFTDQHIGDPTSSSMIYPSDGAHRKLATDILVIGGAYAPGGRPTPSFRISLS
                     VGRAQKDIAVFGDRCWTYSRFNGVSMSQPSPCTEVPLCWERAFGGEEPLRSAPDQRQW
                     EKRNPIGTGFRLTKSKESLDGLALPNLEDPEHLIQSWEDKPAPQNMGCVGRSWMPRSQ
                     YAGTYDEAWQQERMPIPPQDFDDRFFHAATAGLVYPGYLIGGEAVRAFNLSKAAIVEF
                     NLPTMHVTFRGTSRKKTFQAPGLLDTVAFKFDMHRVILVWRYTYTVSLSEPADSVSAD
                     ITVGQGLGHGQCAVRERTRCQSP"
     gene            complement(11265..11837)
                     /locus_tag="PKL17_06225"
     CDS             complement(11265..11837)
                     /locus_tag="PKL17_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44357.1"
                     /translation="MERIEPILIEFGCRFENEIHQLVNTDGTPGVETAKTVSVNSLAE
                     VPMVAARFWGVSLYCVSEDAGEIYIRIFKAGQVGFHVQYNESSSAFRQRIEDSRLAER
                     LTGFLLRMAATLSAKACVYDMESVDGFVDASPETIRALLQSGRFASERDRGLLLLSAD
                     SLPFEEASALAPHHGSVKLSTTGYIVMNFL"
     gene            complement(11905..12450)
                     /locus_tag="PKL17_06230"
     CDS             complement(11905..12450)
                     /locus_tag="PKL17_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44358.1"
                     /translation="MATKIIQLTLTAASPEKPTTRCPGGGDHQWVLKKASGSKSLDDK
                     IQDLKSASKAGTQFEGIAAEHNKKAGTLSDSRDLSEDTDPMVWKCTVAGCHFARQSRE
                     ADHTADSPPGKQSIVEVKATRSLDERSARQLGRNLQAASQGQVAGVIMKVPAGPQRST
                     LVGQIRAVAASLGKTVSIIRV"
     gene            complement(12495..14114)
                     /locus_tag="PKL17_06235"
     CDS             complement(12495..14114)
                     /locus_tag="PKL17_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNK44359.1"
                     /translation="MPTSLAQSILSPSFTPAVRDVGAVIDLLCRGRATPAAVTVALLR
                     VGKAALLPIAQALPTAPKELRPDLVAILGRIVVETGSDPAPLLSLLADGDRKTARAAV
                     VALGKLASKQPAVEAELVRLWPSWTSSADRRALVETLGKIGGAASLVLLDSTQAEPGS
                     VLAMALAQAKQRLVRTGLRHEQIPATERILASVALPQKWPLSLRARAGLSQILVSELA
                     TLGLVPDSVVDQQTLGEPSRVTVGWSRPLSELWKLRTFVDVGFSLPLPVQDFDDIRFS
                     HALASALGKPEVIQLLSALTALPIRYRLELVDQGPRRALLRDLSAQIAARVPQLINDP
                     TDSPWQFDVVQTGAGKLCLELVPKQLVDPRFAYRQKMVAAASHPTLAAALAHVLAAAP
                     GDVVWDPFVGSGGELCEVFLRSPGVRLLGSDVDAEALAAAQANGKAAAAPLELRRGDA
                     LSLWPQGVTCVVTNPPMGRRVCRGDLVPLLEQFVVHVARRLPRGGKLCWLNPLPNQTS
                     HLLADHGMRRKIAQPVDMNGFWAQLELWIAR"
     gene            complement(14124..15266)
                     /locus_tag="PKL17_06240"
     CDS             complement(14124..15266)
                     /locus_tag="PKL17_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNK44360.1"
                     /translation="MPVPSRKSRALRFAASAAMALCVHGLTFGYLAWAGVLSPSISKP
                     LPLIVDGLPEREGESEPLSVESLVDEIEKPPKPSSADTKAAKEREEKDPKGQVVDIAQ
                     PAIEQRPDQSKFLSEYDAMVLHETKGAVGRDKAGAKVAPTPPSPFVPPSPPSKLPPGD
                     PRAELPKNGLLALRGPSGDKLPGPTGQKADVRSLGPDGTLAHQAGQGAVSPSERVGAQ
                     TLPGMGPLRLTPSQESLQRALGQGSGSPDYLGDLDDGDATSLSSRKWKFAAFFNRMKS
                     QVREEWHPDQLLSRHDPSGNIYGSKDRVTMLKVELSLDGRVKDVSVVKPSGVEFLDDE
                     AMSAFRRAQPFSNPPEPLADPDGIIRFQFGFVVQVSGYTSFRVRKY"
     gene            complement(15284..16156)
                     /locus_tag="PKL17_06245"
     CDS             complement(15284..16156)
                     /locus_tag="PKL17_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNK44361.1"
                     /translation="MDQDKSRKNRADAVGRGPLLLLTYAGALEAPETGRRFILDGEEI
                     IIGRSSDATIQVDRDSVSRRHARLSKTEGGWTVQDLQSTNGSYVNDVPIREHKLASGD
                     LLKVGSAIFRYLAGNNLESLLTDELYQIAVRDGLTHSLSRRAFVEAADREVARAHKQS
                     RPLSLLAIDIDHVKQINDNYGHLSGDFVIRELARRIRKKCERYELLCRYEGSQFLLLL
                     PESNQQAAQARAEEIRASAASESFSFEGDQLAVTVTVGVLTLSPEGDTVSLLRQSQEI
                     VQRGKKQGRNRVVS"
     gene            complement(16180..16674)
                     /locus_tag="PKL17_06250"
     CDS             complement(16180..16674)
                     /locus_tag="PKL17_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44362.1"
                     /translation="MNARTTTRFLCSALPCLALGTLAMFSTGCEPPPDNSPAIRITAP
                     NPGDVLPAGQPIKVLFEISGTDSSAGCEAGFAFKLSGSDVKECGRGQVRAYINGVNFV
                     ARVNQVPAADNPWQIPNSAIVGDTAPYLTAGSKRLVFKLFYNDSPGTPVDPQRSVEMN
                     VTLQ"
     gene            complement(16756..17496)
                     /locus_tag="PKL17_06255"
     CDS             complement(16756..17496)
                     /locus_tag="PKL17_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44363.1"
                     /translation="MAREENSLLFSLKEVANTLQDRLKEEEDTRLAAIEAERRAKEDA
                     IRRAKEEEEAKIRAAEDAVRREREEKERKEREDRIRLEEAERRARIEAEARIEEANLR
                     AAAEAIKAKPLPVKLIAAVSAVFILISIVVVVSVKRAQEAAKKEAIAQAIARIEAERK
                     KDRDEAARLQKALTDRLTEAKQLEDQVNAAKSEKERMELAERAKQAAAAASRAAADAA
                     AQRAASKERDRKAKLERCKNADDPLCGI"
     gene            17816..20791
                     /locus_tag="PKL17_06260"
     CDS             17816..20791
                     /locus_tag="PKL17_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNK44364.1"
                     /translation="MDVAWGLAVQPASAATQAAPAQTPAQAHGPGAGAPSPHPGGHGA
                     GPVAGPAVIGPMEKHLRNLRQLTFGGENAEAYFSQDGKKLSLQATTAADRCDQIYQLD
                     VPQPGAAAPKLLPVSFGKGRHTCSYFFPDASRIIFSSTRHLGDGCPPEPDRSKGYVWP
                     LYNYQIYSSLPDGSDVKRLSNTASYDAESTICKDGSVIFTSDRDGDLELYRMNLDGSG
                     VKRLTTAPGYDGGAFFSEDCKSIVWRAARPKNEAEAKEMKSLLAEHLVRPTRMELWVA
                     DADGKNAHQVTDLGAASFAPYFYPASVAGAPNRRIIFSSNNGDPRGREFDLWAINADG
                     SQLEQITFAPDFDGFPIFSPDGKTLAFSSNRGGKSRGETNVFVADWVKSEPQSTKPVP
                     ADQVGRRIGWLAAPEREGRGVGTNGIKAAAQEIASWMQAAGLKPAGEKDGGKPSFFQN
                     VEVVIGVRDAGSSFRLEGAAPRALTSTEATAAAMSSNGAFAGKIRFAGYGISAPESHW
                     DDLHGVDVKGQVALVLRGIPDGKIPSSAARSYSDLRYKAWNLREHGAKAVLFVDPKND
                     NLPKLTLDGPEGGAGIPVAFVSRALVAQLLPLTATAASVQANPHAPAPKVEGPLSHLL
                     LATETAQAAAITATAELSGKVNLARESRQERNVIGYLPASQPPTGEVPALVLGAHYDH
                     LGHGGRTSMAPGQTAVHPGADDNASGTTALLEAARLLGEKDRSFDVYFAAFTGEELGL
                     VGSSKFVSAWPPELSRERVKAMLNFDMVGRLGGSADATIPTVNVQGSDSAAEWKDLVI
                     PTCQQAGLTCKLGGDGYGPSDMTPFYAAGVPVLFFFTGAHPDYHRPTDTADKINALGI
                     LHISRLATEIVRALGEKLKTPGARLTYTKPKGPAPRAGDMGGYGAYLGTIPDYTAMQG
                     AQGGVKLSGARPGSPADKAGVTAGDILVQLGDSKISTLEDMTFALRKYKSGETVDVVV
                     RRGGDQVIKLRATLGTRGQ"
     gene            20839..22428
                     /locus_tag="PKL17_06265"
     CDS             20839..22428
                     /locus_tag="PKL17_06265"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNK44365.1"
                     /translation="MSTSSPNETPSVAGERPSPLKVLEAKETEALAAGGAARVKRQHE
                     NGKLTARERIELFCDKGSFVELDKFVTHRCTDFGMQDQKVLGDGVITGYGMASGRQVF
                     LFAQDFTVFGGSLSGAYAQKICKVMDLAMRVGAPVVGLNDSGGARIQEGVESLAGYAE
                     IFTRNVLASGVVPQLSLILGPCAGGAVYSPAMTDFVAMVDKTSYMFITGPDVVKAVTH
                     EEISKEELGGSRTHASRSGVAHLRCPDEKTALLTMRDLLSYLPQNNAEDPPVVATNDP
                     ADREDAALDSLVPLEATRAYDIKELIRRIVDGGRFLEIQAELAQNIVIGFARMDGRSV
                     GIVANQPAVLAGVLDIAASVKAARFVRFCDCFNIPLITLVDVPGFMPGKDQEYGGIIR
                     HGAKLLYAFVEATVPKVTLITRKAYGGAYDVMASKHVRADINFAYPTAEIAVMGPEGA
                     VNIIYRDELAQSKDPEATRAALVREYRDKFANPYAAASLGYVDEVIRPRQTRAKIISA
                     LHMLRNKRQQNPPKKHGNIPL"
     gene            complement(22451..23365)
                     /locus_tag="PKL17_06270"
     CDS             complement(22451..23365)
                     /locus_tag="PKL17_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK44366.1"
                     /translation="MSEAAASAFRHQIITAGGLRFHVAECGSEDGPPVLLLHGFPECW
                     YSWRHQLAALAAAGFRAMAPDLRGYGESDKPARVKDYSPAQLAQDVVAISNALGYKRL
                     DVVGHDWGGAIAWLTASFAKTKHVVRRLAILNAPHPALFLDQLTFRQLRRSWYMLFFQ
                     LPHLPEWLLRRDDFAFVRKIFSHTQALRKRISDADFAVYKEALSRPFALTSTINYYRA
                     VVRENPFLLKRRVSPVEAETLLIWGERDAALGKELTEGLSQMVPRLQVAYLPAGHFVQ
                     LEQPDEVSRLLVDYLSRPLPLPTAFESP"
     gene            complement(23362..24987)
                     /locus_tag="PKL17_06275"
     CDS             complement(23362..24987)
                     /locus_tag="PKL17_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNK44367.1"
                     /translation="MLAKQTDEVQKWTLPLATIECQNPATLEKLGEVPSLSPADVKAR
                     VERARQAQIAWGQTTFAERRRVLRMLLDHIVAHQADICRLCSVDSGKTMADAAMGEIF
                     PVCEKINYLLEHGEADLKTDSRPSGLLMHKAARVEYHPLGVVGVICPWNFPFHNIFCP
                     TVPALFAGNAVIAKTSEWTAWSAADFQAIFDEVLTKAGYSPDLVQVITGDGETGRALV
                     NSGVEKIFFTGSPENGRKVMSSASETLTPVVLELGGKDPMIICDDADLDQAVGAAMLG
                     VFTACGQMCVAAERLYVFDGIYDRFVDEVVRRVRALRQGPPSQDPGGRFDLGALTMPR
                     QLDIIERQVADAVGKGARVLCGGRRASGLSGQFFEPTVVVDCNHTMDLVQKETFGPVM
                     TIIRVRDEEEAVRLANDTSYGLGSSVFTKNRRRAERIAKGISAGMTVVNDYGIAYMVQ
                     AAPFGGTRISGFGRINGREGLRACCHVKTVVTDKLPIHLPMSLYPIRAATFPMLEGAV
                     RLFYGNGFSERAKGVVQVARSLVEMAKQARSDK"
     gene            complement(25106..25939)
                     /locus_tag="PKL17_06280"
     CDS             complement(25106..25939)
                     /locus_tag="PKL17_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44368.1"
                     /translation="MMRRGGILLGALLSAVLFGPGAGRAGSHGSASHDSAGKSGGKPQ
                     VMLLLSAPSQDEASRGRLLHHVLRGLRGKKVTADPIDVSTAQPGDRWPQLDRCSDSEC
                     QAELCQKSELRQLFSLRIVRDGDDYQVSGQIYDAAMADFASAVETTCVSCDGDQLAAR
                     LVTLTGELVHKAHMRKTGLLEITSMPPGATVRLNGMRLGQTPLTVTTFAGEHRIEVVK
                     PGFVRYQLDVVVEPGRGAAHDANLVIEPGVPQSGDQPQPRRASVGQGEAFGQAVLASQ
                     P"
     gene            complement(25936..29622)
                     /locus_tag="PKL17_06285"
     CDS             complement(25936..29622)
                     /locus_tag="PKL17_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK44369.1"
                     /translation="MSVHNTAQARRCERCGELYPEEEKHTCGSQDEVKPVSSPSVPPP
                     SAPDTTHSSNDDLIGTVIDGRFDILERLSAGGMGVVYRARHVLLDAEVAIKVLLKPQD
                     QDAQYRFLQEAQLASKVKHPNTVVVSDFGVLPDDRSFLAMEFLRGPVLSKVISQGPLE
                     PLRMCRIAVQIARGLQAVHDKGIIHRDLKPDNIFLVDQDGQKDFVKIVDFGIATNRAT
                     AVQVDLDMLSAEKLGAEAQRAMKERHTMPGMVLGTPQYMSPEQTQGFELDPRTDQYAL
                     GCIMYEMLTGVVPFDDASFLTVMFAQASAPVPPMGARAANLKIPESLERVVMRTLAKE
                     PAARFPSMKALEEALQAEIDVLSPTGRQSQVQIQVVKKGWPLWALMATIWGTLLVVGA
                     STLVGWVIYKRQKQAAHDESLAATTRLLEARQRAVEVLKNDLAAKAVELRVGALSGLA
                     ETRDGTLLPVLSPLLKDGELRVQVKAAEILGQLGRREASAQLAPLLGADGVAPLLQTA
                     AAEALDLLGDVRGRQALKKTMASKGDQQAKLRATLYLCGTGDADARKLLTQAVAQGLL
                     SETGQLEVLPLLSRAGDQNARSRLRLRLGGSGNVDQQRQVAMSLAKLSEPSGKQYLRE
                     QSKKPGLDGLIAAVLLAQLEESVDPALFRNVLNAPAATASMQVLAVTGLGYSGQRPDI
                     ELLQARIIPTEPPLQQAAATAIIRLTGADPQVLAMQDISLAERALQGGGGLLPEEALA
                     ILGEVGTGKQVGTLTRVLRDSTDLATRRGAVRALAKVRDRAALTALVSGLEDKDASVR
                     EEVIRALGEAGRRLIAQGQKDVLGEVKGWLGKVVTGPSGREQALARTTLLKLGDESQR
                     TEVLGVLRSGDGDARRQVIESLDKDAATLSAALEDPEDQNRFLAARRLAELNDKRAIP
                     VLRQTLADNPDSPEGILAYALLRRLNVQAEAPKKATELLDSSEPAVRAAAVQAAVEQD
                     SATAALLLERAARDPDKQVRLAAVAEVARLTEGESRPLLRTLMRDRDPEVRAKATAVY
                     QQQKAAAEKPPEPTDAAARPDAGAAQPDAAATQAVQKPDAVEDKPAQLEAIEKLVHAG
                     ASAFKDGSYDKARARLQQANALCGREAQKACAKFSWELGFYLGRSFEGDARYEQAMSE
                     YQKLKSQRGLSKSQRKELSEATGRVQNKLALLTIIHNKKGRCVPEERWLGPGTHELQI
                     KGSPAQTIELRARQKQVIREPGCT"
     gene            29847..>30827
                     /locus_tag="PKL17_06290"
     CDS             29847..>30827
                     /locus_tag="PKL17_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNK44370.1"
                     /translation="MGTDESLDKTAQPDEGPDATLGPDDQPVPTLRLLYSRSIGILTP
                     QRAKLRSEPSAWRIDSRELRIGRGAPPSEVCLADDSQLSRLHASVKASGLQVVLRDLG
                     SSNGCFVGGTRTKEAVLQDGDIIRCGGSLFIFRCEPTKVPDAKIDSLIGQSLLSRRLR
                     HEIALFAPTQCTVLLLGESGTGKEVTARALHTLSGVAGPLIAVNCAAIPETLAESQLF
                     GHVAGAFTGAKAHAGYFRAAHHGTLFLDEVGELPASLQPKLLRALEEGVITPVGSVEP
                     QPCKVRLIAATNRHLQSEVADGKLRGDLYARLVEIVLRLPPLRARREEILL"
BASE COUNT         5990 a        10266 c         8877 g         5694 t
ORIGIN      
        1 agcatgcagc gcgaatggac cgtgacttcg ccggaaaacg gctgattcga catccgcagc
       61 tcgtttttga acgacagccc gttgccccag cgcttgcccg cgcccgccgc attgccgaga
      121 aaccaggcgc catttatttt ttccagctcg tcgggacggg tgactttctt ccaatcatgc
      181 gcaaacgaca gcgacccgag cgttccttcg acgacggaaa atgtcgcctg gcctttgccc
      241 ttgccgccct tgacgctgac gtcgattcga tactcaccca gctccagttt cttggcctca
      301 ccgtcgagaa cggtcagaac caagaccccg ttttcgtcaa gctttccagt gatgtccgag
      361 cttacgccgt cgcgcttggc cagcttgccc gtcacgtccg ccgacggacg cccgagcgcc
      421 atcactttca aatagacttt ttcattgggc cagtactgcg gctggtcggt cgtcaccgac
      481 gtgagatcca ggctcagcgc cgcttggacg cgatcgaccg gaccgaccac tttgctgtgg
      541 tccatgtcga ctttgaagaa cttcgactcg cgaatgcccg tcggcttctt ctttgccgcc
      601 gccgcgcccg gtccaagcat caccgccagt gcaaacaaac acagaagatg caactttttc
      661 atggctcacc atggtaacgc caaggcagcc tgccgtcttt gcccgactcg atctcgatct
      721 atctgcgacg gaaatagacc gtgccgcgat cagcaaagca gggcatgcag aagccgggtc
      781 gcatcgccga ccacatcttt tggcagccac accgaaatcc gcagcggcga cgtcgtcacc
      841 cgcgacggca caaaccccga atcccgtagc tgcgaaagca gcctgccaag cagcaccgga
      901 tcactgccga tccccggtcc gaccgccgtc accatcccca gatcgtcccg cagcaccaga
      961 tccggatcga ctgtgcgcag ctcatcgagc gtccggccaa agtccggtag atcctccagc
     1021 gtcagcgcca cctcgagcat ttcaccgcag agcccgacgt gccgcagact gaccccgtgc
     1081 tcgccgagca cccgcaaaat cgccgcccca tgccgcgact ggatttcgac gatctgattc
     1141 gttccagtca ctgccgtcgc ccggcctcgc tcgatcggtg cttcatcatc cgatcgaatc
     1201 agcgtctgtt tggccgatcc gtgcgtcttg cgggcgtaaa tcacgatccc ggcacgcgca
     1261 gcccactcga ccgcttgggc atttagcact ttggcgccgt gatcggccag ttcctgcatc
     1321 tgccgcgtgc caatcgacgg aagatgtctg gcatcggcca caacgcgcgg gtccgccgaa
     1381 tacaccccgt cgacatccga gtagatttcg cagtccgccg aaagcgccgc cgaaagcgcc
     1441 accgccgtcg tgtccgatcc gccccgtccg agcgtcgtca cctcgcgccg gtagctcatc
     1501 ccttggaaac cggcgacaat cacgatccga tcccgatcca gctcgtcgag gatgcgcacc
     1561 ggtctgattt cgatgatccg cgcccccgag tgccgatcgt ttgtcaaaat ccccgactgt
     1621 gatcccgtaa agctaatcgc atcgtggccc agatccgaca gagccatcgc caaaagcgcc
     1681 atcgaaatcc gttcgccgct cgacatcagc atgtccagct cacgacgcgg cggcgacgtg
     1741 cacacactct ttgccagcgc aatcagctga tcggtggtct tgcccatcgc tgacaccacc
     1801 accaccaccc ggttgccttg cctgaccgta tcggacaccc gctttgcgac gagccgaagc
     1861 cggtccggat cggcgaccga gctgccaccg tatttctgca cgatgatcgg cttttccatc
     1921 gcaggctccg gtttttcaaa cagccaggcc ggcaggggat tgtccgaggg cggcggacct
     1981 tttctgtgct gggtcatcgg acggcatttt gagtgagcac ccaggccagt tcaagattcg
     2041 cgcgccctgg ggaaaaaggt tgacaatcga gatacgcacc tgtagaaagg gcatctgttt
     2101 cgctgactta gcggggcgta gcgcagcctg gtagcgcacc tggttcggga ccagggagtc
     2161 ggaggttcaa atcctctcgc cccgacacga aagccgcagt gggaaaaccg ctgcggcttt
     2221 tttgtttttg ccgtccgcgc aaaaacccag cgctgtgcca atgcccgatt tcgctcaacg
     2281 cgacagccgg acctcggcac ttatcaccgc gtcacctgcg ggcggagctt tcgccaaagt
     2341 ccgacctcgg ctgtcgaaaa tcgccgactg gccggcaaac tcggtcgcgt cgtgcccacg
     2401 gcatacttcg cttccgtagg tgttggcggc gacaaacacg ctccgcgaca aaagcagacg
     2461 aaaccgccaa tagctgtgca gatccagccc ttcttccagc cagttggtgc aaaacgcgat
     2521 gacatctgcc cgcgcgccgt acagaaactc gacgaagcga tcgtcgttta ggtccatgca
     2581 gatgccggcg gtcagcgtcc cccacggaag ccgcacctgc ggatacggcg tatcacccgg
     2641 ctttgcccag gtttcgtcgg cggtgtaaag cagccgcttg cggtagttgg aaaggagcgt
     2701 cccgtcgggc ccaatgatcc acgccgagtt gtaaaagcat ggcgtcggtg cgtcctcggc
     2761 tcgttccgga tagccgcaga ccagcgtaag gccgtagtgc gccgccagcg aagacagcgc
     2821 cgcaaagctc tcgccctgtg ccggctcggc gacacgggcg acatcagcag cgtcggtaaa
     2881 cagatacccg gtcagcgcca tttcgggaca gaccacgagc gtcgcccctc gctctgcggc
     2941 ctgggcacac aaggccagca gccgatcacg cgagccctgt cgatccttgc gatcggcttt
     3001 gaactgaacc gccgcgaccc gcatccgtta ctacagtgcc agaagcgcgg gcagccgccg
     3061 acgcagaagt cgcaaagtca aaagccggcc cagcgtccgc gacacctcag acagcggcag
     3121 ccggctctcg cgggcaatct ccgccactgt ggcgcgaccg ttgcacagcg acagaaccga
     3181 cagctcggcc tgactcagcc ccagccgcaa aagctcactg ctcgacgcgg catagcttac
     3241 gtagatcgcg ccttcggcca catcttgttc aaaccgtcgc cagtcgtcga gccgccgcag
     3301 cccttccaac agaacctgcg ccggatcgag cgcgagcgca ccacccagat cctctgaaag
     3361 cacactggcg ggaagctgcc gggttcgctc aaactcaaac tgtcccgacg gcatgcgcag
     3421 cccttcgcag atcagctcgt agctctgacg cgacagcgcc tgggtcagct cgtcgcggcg
     3481 caaaagcccg gcgcgaacca gccgctgacc cagcagcgat tctggaccct gccccaccgc
     3541 cgccatcatg ccatcggtgg ccacgcgggt ggcgtcgcgc tcgcttggac cgggacgacc
     3601 cagcgactgc gatgcgactg catcaatttc cggctgacga agctgcccgt cgagttcgag
     3661 cagaaaccgc ccgaggcgca agctcggcag accctgcgcc gtcgcctggg caatccagcc
     3721 atcgagaaaa tagaccgaaa gcgcccgtcc ggtcgccgtc accgacagca cgccgcttag
     3781 cttctgccgc gcgatgagat ccaccacttc gaaaagcggc agcgccgtca gatcgcccgc
     3841 gagcactttg ccgcccgcag acgctggacc tgccgatgca gtccgaccaa agcgaacctg
     3901 atcctcgcgc ggctgggtgt cttcatcccc gcgcatgacg tcatcttcga tcggcaacag
     3961 gtcgctgctc tgcaaaagcg ccggatcatc gggatcggcg cgcatcatct ctcgcgccag
     4021 cgcttggatg ctctctgggg ccagcagcgt ctcacctggc tcgaagtcgg agtcttcttc
     4081 aaacggactt ttttcggcct gcggctgccg tgctgtcact ttgtcttcga cgggacgcgg
     4141 caccgcttgt ccactcgcag ggctcatcgg cgacgctggc acggtatgca ccgccgatgc
     4201 atcaaccacc gccggcagcc cacgcagcgt cgcttgccga cccccgcgca cggtctggat
     4261 cggcagaagc cgctgggccg aagccaccac ctggcgcgga aacaccggcc tgaccagcgc
     4321 gtcgacgcca tcgggcaaaa ccgacaggtc atcggtgacg ccgaccagcg ccaacaccga
     4381 aggacacggc accgccgctt cgggcgaaaa cagctgcgac aaaagccagc gaccttctga
     4441 cccttggcgc agcaaatcaa cgaccaaaag cgctggctgc tgactttgga tgactggcag
     4501 cgcctcgctc ccgcgagtga aaccctgcgc caagatgccg acctctgcga acgcagcaac
     4561 cacctgctcg caaaagcgct ggttgtcatc gacgacgaca atacgcggct cgaccatggt
     4621 cgcggacgct aacatgccgg accgggctcc gcaaagccgc atttcgagcc gggtctcacg
     4681 agaaaaatgt gcggaagaag ttaggcgggc acaaacgata cgaagccagc gcttagcgac
     4741 gtcgccgcaa gaggccagcc gccgccgcaa gaatcaacag tccgccagtc gaggtcgcag
     4801 tctgggccgg gaagttgcag ccggttgccg tcgtgccgcc atcgcctggt ggcggggtcg
     4861 tgaccgccat gtccggcgac gaactcatcg ccatgtccgt caccgtcatg cccgccatgt
     4921 cgccaaatcg gctcgggtcc gtcgtcattt cagcgcacga aaaatactgc gagttcagcg
     4981 gaatcgtcgc gggcgggcag gcctgaagat cgtctgccag catccactga atcatccgca
     5041 gcgtgcagct tgtgcagttg ccagcaggca gcgtcacctg cgttgagtag ggccgtggca
     5101 gcgcgcccac cgtggtgtgt ttcaccgtcg ccaagttggt aaacgtcttg ttgccgtctg
     5161 tcgaccactc gacgacaaaa cagcccgggt ggttgatggt ttctttccac gaaaccgtca
     5221 tcgtcgcgcc gggggtgtaa agattcggac cgtttacgat cggcgccggg ccgcacggtc
     5281 cggccttgtt ggcgtcgctg gtgtcgcgcg gcttgggcga tgtcaggatt gcgtgcgcgt
     5341 cgctttgcgc actcatccca agcgtggtca tggtagcggt aaacgcgaac agtcgagcga
     5401 gcgagcggga aaacttcatc ggagagcctc tcttgtgagc taggggggac aagccttgga
     5461 gcaagccgtg acataggcgc tgcccagcgc aggttttgaa agattccaaa tttataggaa
     5521 ttttagctca caaaaaagag cgttttcatg caggagacac aagcagccat ggcggtcttt
     5581 gttcatccaa gcgcagacgt atcggaaaaa gcccagcttg ccgacggcgt gcgagtctgg
     5641 aatcaagccc aagtccgcga acatgccgtg atcggacagg gcacccagat cggcaaagat
     5701 gtatatgtcg atgcaggtgt caccgtcggt gcgcactgca aaatccaaaa cggtgtctac
     5761 ctttaccacg gcgtcaccgt cgaagatggc gtctttttgg gaccgcgcgc gaccacgacc
     5821 aacgacaaga ccccgcgcgc gattttcccc gatggcaggc tccgcggtgc cagcgacttt
     5881 tcggtccagc cgacccgcat ttgttacggc gcagcgattg gtgctggagc cgtccttgtg
     5941 tgcggaatca ccatcggaaa atttgccatg gtggctgcag gttcggtggt cacgcgcgat
     6001 gtacccgcct acggtctttg cttgggcaac ccggcgcggc ttgtcggcgt cgtctgtgcg
     6061 tgcggcgtca aacagtctgc cagtgactcg ttttgtccag cctgcggtgc gccgattccc
     6121 gacgaagcca agtcactttg gacccagctt agccctgggc gctagccgcc aagacaagct
     6181 gaagatagcg cgcgtgcaat tcccggcccg ctgcatcgac ctcgtccaac ttgacaccga
     6241 ggtccgccgg ttcttgccgg ttgcgccacg ccacctgtcc ccgcgcatgc agcgaagagc
     6301 gcagcgccgg caggtggatc accacgtcca cttccgtacc aatccccggc ggctctggac
     6361 aggcaaagct gaccgtcgag cgagaaagac cgatcacctg gtgcggctcg tccgtacttg
     6421 ggttttccag ccgagccgtg acttcgaagc gggggcgata cgtggcgtgc gtcatggggt
     6481 ccttgcggcc acagccaaaa cattctgggc cgcaaagaat cgtatcacaa actactcaat
     6541 tatttgacag acaattggga agcaaacacc cccaccgcaa tgcatggcca aaggccctgg
     6601 ggccgcccaa acccaggatc aacaaacccc caccgaagcc cgcagcagac cgtctatacg
     6661 ctacagcgtg gcgaggaacc gttcgatgat ccgctgcgtg cgctgctgaa gcggcgtcgg
     6721 tgcgatctcg aatgcgtgcg gcgccatcgg cacttcgcac agatgaaccc gcctcgcgcc
     6781 cgcttctttt agtcgctgat agaagttgtg cgcctcgacg atcggaatca tcacgtcatg
     6841 ctcgccgtgg atgagcaaaa ggggcggaat gtctgccgat agatagctga caggctgcgc
     6901 ccgccgaaag acatcggcgt cttcgcgata gcgacggcga aacaccagtt cttccagcag
     6961 atagtgcgca atcggatgcg gatggtgttc aaaaatgccg gtaaagtcgg tcagtccgta
     7021 caggatcact ccgccgcgca cccgacagtc tttgtcctcg aagcccggct gcagcgatcg
     7081 atccgatcct gaacacgcga gcatcgccgc caggtgtccg cccgccgagt ttcccatcgc
     7141 aatcgcgaaa ggggtgccgc cgtattcggg accgtgctcg cgcacccacg ccaccgcagc
     7201 tttgcagtcg tgaatcgccg ccggaagcgg gaagcgtggt gccagccgat agctcaccgc
     7261 aaacaccacg tagcccgccg ccgccaaatc gaacatcaga aacggtgact ggcgcttgct
     7321 gccgaccacc caagcgccac cgtggacaaa cacgatcgac ggcaacagct ctgccgggcg
     7381 gtttttgggc cgatagacat cgagccgcag ccgcacccca tcgacctgcc ggtagacgac
     7441 atcgcggatc acatccacct tgccaatcga gggcgtgcgc agaaaaagcg acggccacag
     7501 cgagggagca cggccatctt cgagatcctg gccttcctcg gcatgatcgg gaaatgggtg
     7561 ctcggcatcg acaatcggct gaccatcgac gaccggcagc acactgcgca gctgccaaag
     7621 atagcgaatc agaagtcccc acgacaggat gtgaatcagc agcccgacca gacccaccgt
     7681 cgttgaaaac gcgccaaagt gcgcaaacag cagcatcgtc aaaagcccgg caaagacgat
     7741 ctgcggcaca aacaaagaca ccaaaagcag cggcaccgag gcaaccaggc caatcgtctg
     7801 cgacagccag cgcggacgca ccactccgac ggcaagaaac agcaggatca gcgacaaaaa
     7861 caggtacagc gcagaaagca tcattccctc gtgaagcgcg gcttttctct taagtcatgc
     7921 cacacagcgc aagcttttgg gatcgggtca gtcgcttgat cgcagcttct cgacgcagcg
     7981 ccgcacgccg atcggccacc gactcggcat acaaaagctc gaccggcaag cgcgaccggg
     8041 tgtacttggc gctgccacgg gcgtgggcca agagccgctt gggcaaagaa atcgcacacc
     8101 cggtgtagat ggtcccatcg gcacagcgca gcatgtagac acaccaaccc gtgatcgccg
     8161 atggctcgac agaagcgctg ggcggcggtc gtcgggcggt ttcaattgcc tgcattgacg
     8221 ttcgcacagg cggaatgcta gcgtgcttgg cccagggcat gcgagtcgat accggaagaa
     8281 agctgtttgc aggcgtgcgc gttgatggcc gaatgcgtga gcagctcgac aagtgcccac
     8341 agcgagaccg ccaatacttc gagtctgccg acggcaaata cctgcaagtg ttgcgcagcg
     8401 gcaaagacag cttcatcggc aaggtcttag aacccgccac cgaagcgcgc agcctcgaag
     8461 acattcgtcg caatgtctgg tcgctgctcc agcgcgtctg tccgggccgc cgtgacgaag
     8521 gcgaaattcg cctgtacgcg ctcgaatcgg aagaaccgac gatgctgcac ggtgggctgc
     8581 cacaacccgg caatccctac agcaacgaag aagacgacga cttccgctag ctttccgttc
     8641 cgaaagtaac cgtcatttcg ccgcggggtt tcatcgcgtt cgaacgtggc gcgtgtttgg
     8701 cggaccaggc ccgatgagcc tttagatcgt gttcttcttg ttctgcatca ttgggtcgga
     8761 tttccggacg acgttctgtc cttcgacctt cacatcgaac gaatagttga cggggtccgc
     8821 ctggcccttt gtgacgttcg acttgatgcc accgatggaa ccggcttcat ccccagtgct
     8881 aagaccaaac gatgaattct tcagcatgat ggagttccca tcgcacttca cggtcgtgct
     8941 cccactcgtc gtgtcggacg acatcgcaac gttcgggtat gggatcggca ccggcccccc
     9001 tggcgtcggt gtcttgcaca cgtcaagctc ggtcttggcg agtcccatgc tccccttgtg
     9061 gacgactgtt tccggttcat tcacagctac ggtgactggc atcgcgttct ctctcgtact
     9121 gcgcactggc catgcccgag cccctggcca actgtgatgt ctgccgatac ggagtccgca
     9181 ggctcggaca gtgaaaccgt ataggtatag cgccagacca ggatcacccg atgcatatcg
     9241 aatttgaagg cgacggtgtc cagaaggcca ggcgcctgaa acgtcttttt ccgagacgtg
     9301 ccgcggaatg tgacgtgcat tgtgggcaga ttgaactcca caatcgcagc ctttgacagg
     9361 ttgaacgcac gcacagcctc tccgccaatc aaatatcctg ggtacacaag accggcagtg
     9421 gctgcatgaa agaaccgatc atcgaagtcc tgcggcggaa tcggcattcg ctcctgctgc
     9481 caagcttcat catacgtgcc cgcatactga ctgcgcggca tccaactcct tccgacacag
     9541 cccatgtttt gtggtgccgg cttgtcttcc cagctctgga tgaggtgctc ggggtcctcc
     9601 aggttcggca gcgccagacc atccagtgat tccttgctct tggtcaaccg gaagccggtc
     9661 ccgatcgggt tccttttttc ccactgacgt tggtcaggag cgctccgaag gggctcctca
     9721 cctccgaaag cgcgctccca gcacaagggc acttccgtgc acgggctagg ctgtgacatg
     9781 gagaccccgt tgaaccgact gtaggtccag cagcgatcac cgaacaccgc gatatctttc
     9841 tgcgcgcgcc ctaccgacaa gctgatacga aaggatggcg taggccgccc acctggtgca
     9901 taggctccgc cgattaccaa aatgtctgtc gccaattttc gatgtgcgcc atcgctggga
     9961 tagatcatgg acgaactggt gggatcacca atgtgctgat cggtgaagca tattggctcc
    10021 tgctcttcgg caatctgcag caaaccatcc gaacgaatct cgaaagtggc cttgagcagt
    10081 gcggttcggc cctcacttcc atctttgtcc aagaatgaca gcagggcccc gacaaagggt
    10141 gtgctgtgta aaaccatcat cgcgatctcg aatccctgca ggcattctgc acgcagagcg
    10201 cccctctcgt accctgttcg gaagagcaga agaccaagca gcgctcactt cgggcataac
    10261 cacgcgccca ggcaatcgcc gctacaccca gcaaaagagg catggaggca gcgccaatgt
    10321 ccccgataca atcggcacaa tgaagcacct cgggcgagtc cagggtgcgg caccgggcga
    10381 gcatccattc atgggcacga tacgcctcgc cgttcagatc gcagtagacc tggccgatgt
    10441 ccatctgact ccaccgagcc atgcggagtg catctcgaat gcaatcggtc aggcctttgc
    10501 ccagacacgg cagttcgctc aggacaggtg ccggttcttt cgcgatgccc agcccagcaa
    10561 cagtacagta cacgttggac ccctcggtct gggcgcaccg aagatcctct agcgcgacgc
    10621 aagcactcgc ttctcctgga atcagtccac ggggctgcga gcgccctttg agtcgagcac
    10681 tctgctctaa acgcaacagg gtcggaatgt cgtggagcga gtcgatgccc cacacaagct
    10741 cgatcgcgcg tgggtcgtca gcgagtcgct cctgtgcatc gcgcacggca atcataccgc
    10801 cggcgcttcc ttgcggatag acgtgcattc gcgtcggagc cgaacgaccg atatcagaaa
    10861 gcacgctctg tagtgccaag aagctctcgg aggacagcgt ctcgccatcc cgcaacacca
    10921 ccgagagacc gagcggacga cttgttcccg tcgggtcacg cggagcaagc gcctggcgaa
    10981 gtgtatgctg ggcaatctga cggagccgct ctgccggacg attcgcaggc gcaaaccccg
    11041 gaggtatccg agctgttatg gttggcccgc cgtcgccaga aacaacgtcg ggaagttcgc
    11101 agaacctgac gattccggca cgtagggcgg cacaagtctg ttgcgccgtg tatcccacgg
    11161 gggaaaccat gccaaccccc gtgaccgcaa gcgtcgctgt caagctaaac cactttcttt
    11221 gcctaagaga tgggctggcc gtttgccttc gggatggcct cgcatcaaag gaaattcatg
    11281 acgatgtagc cggtcgttga cagtttcact gagccatggt gtggcgcgag cgccgatgct
    11341 tcttcaaacg gcagtgaatc agcggacaaa agcagcagcc cgcggtcgcg ctccgaggca
    11401 aaacgtccag actggagcag agcccggatc gtctcgggac tggcgtctac aaacccatcg
    11461 acggattcca tgtcgtagac gcaggccttg gcagatagcg tcgcagccat ccgcagtaga
    11521 aagccagtca gccgctcggc aaggcgcgag tcctcgatac gctgacggaa agccgagctg
    11581 ctttcattgt actggacgtg aaaacccacc tgtccggctt tgaagatccg aatgtagatt
    11641 tcgccagcgt cttccgaaac gcaatacagc gagacacccc aaaagcgcgc cgctaccatc
    11701 ggtacttcag caaggctatt cacgctcacc gtcttagcgg tttcgacacc tggcgtccca
    11761 tctgtgttga cgagttgatg aatctcgttt tcgaatctgc aaccgaattc gatcagaatc
    11821 ggctcgatcc gttccacgat agacttgagc gacagcgtga tgagggaaaa ggtcgcgtcg
    11881 taatggtgtg ccatgctctt cgccttatac gcggatgata gacacggtct tgcccaggct
    11941 ggcggccact gctcggatct gacctacgag cgtgctccgc tgcggccccg ccggaacctt
    12001 catgatcaca cccgcaactt gcccctggct tgccgcctgc agattccgcc caagctggcg
    12061 cgcactgcgc tcatcgagtg accgcgtcgc cttcacctcg acgatggact gcttgccggg
    12121 tggggaatcc gccgtgtggt cagcttcacg cgactgcctt gcaaagtggc aacccgcgac
    12181 cgtacacttc cacaccatgg ggtcggtatc ctcgctgaga tcccgactgt ccgatagcgt
    12241 acccgccttt ttgttatgtt ccgctgcgat tccctcaaat tgggtgccag ccttggaggc
    12301 gcttttcaaa tcttggatct tgtcatcgag agacttggac ccggacgcct ttttgagaac
    12361 ccactggtgg tcaccgcctc ccggacaccg ggtggtcggc ttctccggcg aagccgcggt
    12421 cagtgtaagc tggatgattt tcgtcgccat ctgcgcatgc ccttttccgg acagctattg
    12481 caaatatgtc ctcactagcg cgcaatccat agctcaagct gggcccagaa gccgttcatg
    12541 tcgacgggct gggcgatctt gcggcgcatg ccgtggtcgg ccaaaagatg gctcgtctga
    12601 ttgggcagcg gattgagcca acacagcttt ccgccccgcg gcagccgtcg cgccacatgg
    12661 accacgaact gttccaaaag tggcaccaga tcgccccggc agacccgtcg ccccatcggt
    12721 ggattggtca ccacgcaagt cacgccttgc ggccacagcg acagcgcatc gcctcttcgc
    12781 agctccaacg gagcggctgc cgctttccca ttcgcctgcg ccgccgccag cgcttccgca
    12841 tcgacatcac ttcccagaag ccgcacgccc ggactgcgca aaaatacctc gcacagctcg
    12901 ccccctgatc caacaaacgg gtcccacacc acatcgcccg gtgccgccgc aagaacatgc
    12961 gcaagcgccg ctgccagcgt cggatgagac gccgctgcca ccatcttttg ccgatacgca
    13021 aagcgcggat cgacaagctg cttgggtaca agctccagac agagtttccc cgcgcctgtc
    13081 tgcaccacgt caaactgcca cgggctgtcc gtcggatcat tgatgagctg gggcacccgc
    13141 gcagcaatct gtgccgacag atcgcgcaag agcgcccgcc gcggtccctg gtcgactagc
    13201 tccaaccgat agcgaatcgg taaagcggtc agcgccgaca aaagctggat cacctccggc
    13261 ttgccaagcg cagaagccag cgcgtgcgaa aaccgaatgt catcgaaatc ctgcaccggc
    13321 aaaggcagcg aaaagcccac atcgacaaac gtccgcagct tccacagctc tgaaagcggc
    13381 ctagaccagc caaccgtcac ccgactcggc tcccccagcg tctgctgatc gaccaccgaa
    13441 tcgggcacaa gcccaagcgt cgcaagctca gaaaccagaa tctgtgaaag tccggcccgc
    13501 gcccgcagcg aaagtggcca cttttgtggc agcgccaccg atgccaaaat ccgctcggtc
    13561 gctgggatct gctcgtggcg caggccagtg cgcacaagcc gctgcttggc ctgcgcaagc
    13621 gccatcgcca gcacggaacc cggctctgcc tgggtcgaat ccaagagcac aagcgacgcg
    13681 gccccaccga ttttgcccag cgtttcgaca agcgcccgcc gatctgccga cgatgtccag
    13741 ctcggccaca gccgcacaag ctcggcttcc acagcgggct gtttggacgc aagcttgccg
    13801 agcgccacca ccgccgcacg cgccgttttc cgatccccat ctgcaagaag cgacagaagc
    13861 ggcgccggat cactgccggt ctcgaccaca atccggccca agatggccac caggtcaggc
    13921 cgcagctctt tgggggcagt tggcagggcc tgcgcgatcg gcaaaagcgc cgcttttccc
    13981 acccgcaaaa gcgccaccgt caccgcggcg ggcgtcgcac ggcctcggca caaaaggtca
    14041 atcaccgcgc cgacgtcacg cacggctggg gtaaacgacg gcgacaatat cgattgggca
    14101 agggaggtcg gcatgggcga aggctagtac ttgcgaacac ggaacgaggt gtagcccgac
    14161 acctgtacca cgaagccaaa ctgaaaacgg atgattccgt cggggtccgc cagcggctcg
    14221 ggcgggttgg aaaacggctg tgcccgacga aacgcgctca ttgcttcgtc gtccagaaac
    14281 tcaacccccg acggcttgac cacgctgacg tctttgaccc ggccatccag cgacagctcg
    14341 actttcagca tcgtcacccg atctttgctg ccatagatgt tgccgctcgg atcgtggcgc
    14401 gacaaaagct gatcgggatg ccattcctcg cgcacttggc tcttcatccg gttgaaaaac
    14461 gctgcaaact tccacttccg cgagctaagc gacgtcgcgt ccccgtcgtc cagatcgccc
    14521 agatagtccg gggaaccgct gccctgacca agcgcccgct ggagcgactc ttgcgacgga
    14581 gtcagccgca gcggccccat ccccggcagc gtctgcgcgc ccactcgctc gctcggtgac
    14641 accgcgcctt gtccggcctg atgcgccagc gttccgtcgg gaccaagcga tcgcacgtcc
    14701 gccttttgtc ccgtcggccc cggcagtttg tcgccacttg gaccccgcag cgccaacagc
    14761 ccgtttttgg gcagctcggc gcgcggatcg cccggcggca gcttgcttgg cggggatggc
    14821 ggcacaaacg ggcttggcgg cgtcggtgca accttggccc cggctttgtc acggcccact
    14881 gctcccttgg tttcgtgcag caccatcgcg tcgtattccg acagaaactt cgactgatcg
    14941 gggcgctgtt cgatcgccgg ctgggcgatg tcgaccacct ggcctttggg atctttttcc
    15001 tcgcgctctt tggcagcttt ggtgtcggca gaacttggct tgggaggctt ttcgatctcg
    15061 tcgaccagcg actccaccga aagtggctcg ctctcgcctt cgcgttctgg cagcccatcc
    15121 acaatcagcg gcagcggctt gctgatcgat ggtgacaaca cgcccgccca cgccaaatag
    15181 ccaaacgtca gaccgtggac acacagcgcc atcgctgccg aagcagcaaa acgaagcgcg
    15241 cgcgacttgc gggacggaac aggcacgggt tgcttggtga agattaactc accacgcgat
    15301 ttctgccctg cttcttgcca cgctgcacaa tttcctggga ctggcgcaaa agagaaacgg
    15361 tgtcgccttc gggcgaaagc gtcaacacgc caaccgtcac cgtgaccgct aactgatcgc
    15421 cttcaaacga aaagctctca ctcgctgccg aggcgcggat ctcttcggcc cgcgcttggg
    15481 cagcttgctg attcgattcc ggcagcaaca gcaaaaactg ggagccttcg tagcgacaca
    15541 aaagttcgta gcgctcgcat tttttgcgaa tccgccgcgc cagctccctg atgacaaaat
    15601 cgccggacag gtgcccgtag ttgtcgttga tctgtttgac gtggtcgata tcaatcgcga
    15661 gcagggacaa aggccgtgac tgcttgtggg cccgcgccac ctcgcgatcc gctgcttcga
    15721 cgaatgcccg acggctcagt gaatgcgtca ggccatcgcg caccgcaatc tggtacagct
    15781 cgtcggtcag cagcgactcc aggttgtttc cagccaggta gcggaagatc gcactgccga
    15841 ctttcagaag atcgcccgag gccagcttgt gctcgcggat cggcacatca tttacgtagc
    15901 tgccgttggt cgattgcaga tcctgcaccg tccaaccgcc ctcggttttc gacaggcgcg
    15961 catgccgccg cgaaaccgag tcccgatcga cctgaatggt cgcatcgctg ctacgaccga
    16021 tgatgatctc ctcaccatcg aggatgaacc gacgaccagt ctcgggggcc tcgagcgcgc
    16081 ctgcatacgt cagaagaagc agcggacccc gaccgacagc gtcggcgcgg ttcttgcgag
    16141 acttgtcttg gtccatgtag aaagcccctc ggatcggact tattgcaagg tcacgttcat
    16201 ctcgaccgac cgctgcggat cgaccggcgt gccaggcgaa tcattataga acagcttgaa
    16261 gaccagtcgc ttgctgccag ccgtcaggta cggcgccgtg tcaccgacaa tcgccgagtt
    16321 cgggatctgc cacggattgt ccgccgccgg cacttggttc acgcgggcga cgaagttcac
    16381 gccgtttatg tacgcgcgaa cctgtccgcg accgcactct ttgacatccg agccgctcag
    16441 cttgaacgca aagcctgctt cacaacccgc gctgctgtcg gtgcccgaga tttcaaacag
    16501 caccttgatc ggttgacccg cgggcaggac atcccctggg tttggcgccg tgatccgaat
    16561 cgccggagag ttgtcgggcg gcggctcgca gcccgtcgaa aacatggcca gggtgccgag
    16621 cgcaaggcaa ggcagtgcag agcataggaa acgagtcgtg gtccttgcgt tcatcgcagt
    16681 gtcctttcgg tgaagaaaca agagcaaatg ctcttaagca ggtaggttca aacgtaagcg
    16741 aaggcgcggg gtcgcttaga taccacagag cgggtcgtcg gcgttcttgc agcgctcgag
    16801 cttggctttg cgatcgcgtt ctttcgaggc cgcgcgctga gctgcggcgt ccgctgcggc
    16861 gcgagaggct gccgcggctg cctgcttggc tcgctcggcc agctccatcc gttctttctc
    16921 gctcttggcc gcgttgactt gatcttcgag ctgcttggct tccgtcagtc gatcggtcag
    16981 cgctttttgc agacgggcgg cttcgtcgcg gtccttcttg cgctcggctt cgatgcgcgc
    17041 aatggcctgg gcgatcgcct cttttttggc cgcttcctgg gcgcgcttga cgctgacgac
    17101 aaccacgatg ctgatcagga tgaacaccgc cgacacagca gcgatcagct tgaccggcag
    17161 tggcttcgcc ttgatggctt cggcagcagc acgcaggttg gcttcttcga tgcgagcctc
    17221 tgcctcgatg cgagcgcgac gctccgcttc ttccagacgg atgcgatctt cgcgctcttt
    17281 gcgttccttt tcttcgcgct cacgccgtac agcatcctcg gcggcccgaa tcttcgcctc
    17341 ttcttcttcc ttggcacgac ggatcgcatc ttctttggcg cgacgctccg cctcgatggc
    17401 agcgagacgg gtgtcctcct cttccttcag acgatcctgg agggtgttgg cgacttcctt
    17461 gagcgaaaac agtagcgagt tctcttcacg ggccatggga actgcgtctc cttgtcaggc
    17521 tggtttcggc taaaagccgc cagctgcggg cctttcttcg acggaaaatc tgcttgacac
    17581 tataccactt gaggttccga cgccgccttt gattttgcag ctatgcgaat cccctgcact
    17641 tttgccggat gtcgcagcag gcggctgccc aaaaaccgtc gattccaccg caaaaacgac
    17701 cgcgagtcgt gcagcgcgaa gcggccacga gccttgacct gcgtggttgc caagggtagg
    17761 acatgccatg actttttcgt cctcctcttt aacccttttc tccctctgtg tcacgttgga
    17821 tgttgcgtgg ggacttgcgg tgcagcccgc ctcggcggcg acgcaagcgg cacccgcgca
    17881 gaccccagcg caagcacatg gaccgggcgc aggggccccg tctccgcatc ccggtggaca
    17941 tggcgcgggc ccggtggcgg gtcctgccgt cattggtccg atggaaaagc acctgcggaa
    18001 cctgcgccag cttacgttcg gcggcgaaaa cgccgaggcc tacttttccc aagacggcaa
    18061 aaagctcagc ctgcaagcaa cgacggcggc ggaccgctgt gaccaaatct atcagcttga
    18121 tgtcccgcag cccggcgcgg cggcgcccaa acttttgccg gtgagctttg gcaaaggtcg
    18181 ccacacttgc agttactttt tccccgacgc aagccgcatc atcttttcgt cgacgcggca
    18241 cctgggggac ggttgccctc cggagcccga tcgcagcaaa ggctatgtct ggccgctcta
    18301 caactaccag atttattcgt cgctccccga cggaagtgat gtcaagcggc tatcaaacac
    18361 cgcaagctac gacgccgaat cgaccatctg caaagatggc agcgtcattt tcacttccga
    18421 ccgcgacggt gatcttgaac tttaccgcat gaatctcgac ggcagcgggg tcaagcgttt
    18481 gaccaccgcc ccaggttacg acggcggcgc cttcttttcg gaagactgca agtcgatcgt
    18541 ctggcgggcc gcccgtccga aaaacgaagc cgaagccaag gaaatgaagt cgctcctggc
    18601 cgagcacttg gtccgcccga cgcgcatgga gctgtgggtc gccgatgccg atggcaaaaa
    18661 tgcccaccaa gtcaccgacc tgggtgccgc ttcgtttgca ccgtattttt acccggccag
    18721 cgtggccggc gcgccaaacc gccgcatcat cttttcgtcc aacaatggcg atccgcgcgg
    18781 acgagagttt gacctttggg ccatcaacgc cgacggctcg cagcttgagc agatcacctt
    18841 tgcacccgac ttcgacggtt ttccgatctt ttcgcccgac ggcaagaccc tggcgttttc
    18901 gtcgaaccgc ggtggcaagt cgcgcggcga aaccaacgtc tttgtcgccg attgggtgaa
    18961 gtctgaaccg cagtcgacaa agcccgtgcc cgccgaccag gttgggcgcc gcatcggctg
    19021 gctcgctgcc cccgagcgcg aaggccgtgg cgtcggcaca aatggcatca aagccgccgc
    19081 gcaagagatc gcaagctgga tgcaagccgc agggcttaag ccggcagggg aaaaagacgg
    19141 cggcaagcct tcttttttcc agaacgtcga agtggtgatc ggcgtccgcg atgccggctc
    19201 gtcgttccgt ttggaaggcg cggcaccacg cgcgctcacc agcacagaag ccacggcggc
    19261 ggcgatgtct tcaaatggcg cttttgccgg caagatccgc tttgctggct acggcatcag
    19321 cgctccagaa tcgcactggg acgacctcca cggcgtcgat gtcaaaggcc aggttgccct
    19381 ggtgctgcgc ggcattcccg acggcaagat cccatcgtcg gcagcccgct cgtattcgga
    19441 tcttcgctac aaagcgtgga atctgcgcga acacggtgcc aaggcggtgc tgtttgtcga
    19501 tcccaaaaat gacaacctac ccaagctgac tctcgatggg cccgaagggg gtgccggcat
    19561 cccggtggcg tttgtcagcc gcgccctggt tgcgcagctt ctgcctttga ccgccactgc
    19621 ggccagcgtg caggccaatc cgcatgcgcc ggctccgaaa gtcgaaggcc cgctgtccca
    19681 cctgctcttg gcgaccgaaa ccgcgcaggc cgcggcgatc acagcgacgg cggaactttc
    19741 gggcaaagtg aaccttgcgc gcgagtcgcg gcaagagcgc aacgtcatcg gctatctgcc
    19801 cgccagccag ccgcctaccg gggaggttcc ggccctggtc cttggtgctc actacgacca
    19861 tttgggccac ggaggccgca cgtcgatggc gccgggtcaa accgcggtgc atccaggcgc
    19921 cgatgacaac gcctcgggca ccacggcgct cctcgaagcc gcgcggctgc tcggggaaaa
    19981 agaccgcagc ttcgatgtct acttcgccgc ttttaccggg gaagagctgg gcttggtggg
    20041 gtcgagcaag ttcgtctccg cgtggccacc tgagctcagc cgcgagcggg tcaaagcgat
    20101 gctcaacttc gacatggtgg gccggctcgg tggatcggcg gacgcgacga ttcccactgt
    20161 caatgtccag ggcagcgact ccgccgcgga atggaaagac ctggtgatcc cgacctgcca
    20221 gcaagccggc ctgacctgca agctcggcgg cgatggatac ggacccagcg acatgacgcc
    20281 gttttatgcc gccggagttc ccgtcctgtt tttctttacc ggcgcccacc cggactacca
    20341 ccgcccaacc gataccgccg acaagatcaa cgcgctcggc atcctgcaca tcagccgctt
    20401 ggcgactgag atcgtccgtg cgctcggcga aaaactgaaa acccccggcg ccaggctcac
    20461 ctataccaag cccaaaggac cggcaccgcg cgcaggtgac atgggcggct acggcgcgta
    20521 cctgggcacg atccccgact acaccgcgat gcaaggcgcc caaggtggcg tcaagctgtc
    20581 gggtgcccgt cccggcagcc ccgctgacaa agccggtgta accgccggag acatcctggt
    20641 ccagctcgga gacagcaaga tttcgacgct ggaagacatg acgtttgcgc ttcgcaaata
    20701 caagagcggc gagacggttg acgtggtggt gcgccgcggt ggcgaccagg tcatcaagct
    20761 gcgggccacc ctcggaaccc gcggccaata gcttcgctta gaaaaccaaa cccgtttgcg
    20821 ataaggtctt gtccactcat gagcacgtcg agccccaacg aaactccgtc tgtcgctggc
    20881 gagcgcccct cgccgctcaa agttcttgaa gccaaagaga ccgaagccct tgctgccggt
    20941 ggtgcggcgc gggtgaaacg ccaacatgaa aacggcaagc tgaccgcccg cgagcgcatc
    21001 gagctgttct gtgacaaggg cagcttcgtc gagctagaca agttcgtcac ccatcgctgc
    21061 accgactttg gcatgcagga tcagaaagtg ctcggggacg gcgtcatcac cggctacggc
    21121 atggcatctg gccgccaggt ctttttgttc gcgcaagact tcaccgtgtt tggcggatcg
    21181 ctgtccggtg cttacgcgca aaaaatctgc aaggtcatgg acctggcgat gcgggtcgga
    21241 gcgccagtgg tcgggctcaa tgactcgggc ggcgcgcgga ttcaagaagg cgtggaatcg
    21301 ctggctggct acgccgaaat ctttacccgc aacgtgctcg cgtcgggtgt ggtgccgcag
    21361 ctttcgctca tcctgggacc gtgtgccggc ggcgcggtct attccccggc gatgaccgat
    21421 tttgtcgcga tggtcgacaa gaccagctac atgttcatca ccggcccgga cgtcgtcaaa
    21481 gctgtcaccc acgaagaaat cagcaaagaa gagctgggcg gttcgcgcac ccacgcatca
    21541 cggtcgggcg tcgcacacct gcgctgtccc gatgaaaaga ccgcgctttt gacgatgcgc
    21601 gatcttttgt cgtatctgcc gcagaacaac gccgaagacc cgccggttgt ggccacaaac
    21661 gacccggcgg atcgtgaaga cgcggcgctc gatagcttgg tgccgctcga agccacccgc
    21721 gcctatgaca tcaaagagct gatccgtcgc atcgtcgatg gcggacgctt tctcgaaatc
    21781 caggccgagc tggcacaaaa catcgtcatc ggctttgccc gcatggatgg gcgctcggtc
    21841 gggattgtcg ccaaccagcc ggcggtgttg gccggtgtgc tcgacattgc ggcgtcggtc
    21901 aaagcggctc gttttgtgcg gttctgcgac tgctttaaca tcccgctgat tacgctggtc
    21961 gacgtaccgg gctttatgcc gggcaaagac caggaatacg gcggcatcat ccggcacggc
    22021 gccaagctgc tgtatgcgtt cgtcgaagcg acggttccca aggtgacact gattacccgc
    22081 aaagcctacg gcggcgctta cgacgtcatg gcgtccaagc atgtgcgggc cgacataaac
    22141 ttcgcctatc cgacggcaga aatcgcggtc atgggccccg agggcgcggt caacatcatc
    22201 tatcgcgacg agctggccca gtccaaagac ccagaagcga cgcgcgcagc ccttgtgcgc
    22261 gaatatcgcg acaagtttgc caatccgtat gcggcggctt cactcggata cgtcgacgaa
    22321 gtgattcggc ctcggcagac gcgcgccaag atcatctcgg cgctccacat gctgcgcaac
    22381 aagcgccagc agaacccgcc caaaaagcac ggcaacatcc cgctgtagcc caagccgacc
    22441 cgcggcagac ttacggcgat tcaaaggcgg tcggcagcgg cagcggacgg ctcaggtagt
    22501 cgacgagcag ccgactgact tcatcgggct gttcaagctg cacaaagtgg cccgccggca
    22561 gatacgcaac ctgtagccgc ggcaccatct gcgaaagtcc ttccgtcagc tccttgccaa
    22621 gtgcggcgtc ccgttccccc cagataagca gcgtctcagc ttccacgggt gaaacccgcc
    22681 gcttgagcaa aaacggattt tctctgacga cggcgcggta atagttgatg gtcgacgtca
    22741 gtgcaaaagg ccgcgacagc gcttctttgt acacggcaaa gtcggcgtcg ctgattcgct
    22801 tgcgcagcgc ctgggtgtgc gaaaaaattt tgcgcacaaa cgcaaagtcg tcgcgccgca
    22861 gcagccactc gggcaggtgc gggagctgga aaaacagcat gtaccagctc cgccgcagct
    22921 gccgaaacgt aagctggtcc agaaacagcg ccgggtgcgg ggcgttcaga atcgccagcc
    22981 gccgcacgac atgcttggtc tttgcaaacg aagcggtcag ccaagcaatc gcgccgcccc
    23041 agtcatgacc gacgacatcc agccgcttat agcccagcgc attgctgatc gcgacgacat
    23101 cttgcgcaag ctgggccggc gaatagtcct tcacgcgggc cggcttgtcc gattcaccat
    23161 agccccgcag atccggggcc atcgcccgaa aacccgccgc cgcaagcgcc gcaagctgat
    23221 gacgccacga ataccagcac tcgggaaagc cgtgcaaaag cagcaccggt ggcccatctt
    23281 cactgccaca ctcggcgacg tggaagcgca gtcccccggc ggtgatgatc tggtggcgaa
    23341 aagccgacgc tgcggcctcg ctcacttgtc gctccgggct tgttttgcca tctcgaccaa
    23401 gctgcgcgcg acttgcacga cgcctttggc ccgctcggaa aacccgtttc cataaaacag
    23461 ccgcacggcg ccctccagca tcgggaaagt cgccgcccgg atcggataca gcgacatcgg
    23521 caggtggatc ggcagcttgt ccgtcacgac ggtctttacg tggcagcaag cccgcagacc
    23581 ttcccgcccg ttgatgcgcc caaaacccga aatgcgcgtc ccgccaaacg gcgccgcctg
    23641 caccatgtag gcgatgccgt agtcatttac gaccgtcatc cccgccgaga tgcccttggc
    23701 gatgcgctcc gcgcgccgac ggttcttggt aaacaccgac gaaccgagtc catagcttgt
    23761 gtcgttggcg agccgaaccg cttcttcttc atcgcggacc cggatgatgg tcatcaccgg
    23821 cccaaatgtt tctttttgca ccagatccat cgtgtggttg cagtccacca ccaccgtcgg
    23881 ttcgaagaac tgtcccgaaa gcccgctggc ccgccgtccc ccacacagca cacgagcgcc
    23941 cttgcccacc gcgtcggcga cttgccgttc gatgatgtct agctggcgcg gcatcgtcag
    24001 cgcccccagg tcaaaccggc cccccggatc ttgcgatggt ggaccttgcc gcagtgcccg
    24061 cacgcgccgc accacttcgt cgacaaatcg atcgtagatg ccatcaaaga cgtacagccg
    24121 ctccgccgcg acacacatct ggccgcaggc ggtaaacacc ccgagcatcg ccgcacccac
    24181 cgcctgatcc agatccgcat cgtcgcagat gatcatcgga tctttgccgc ccagctccag
    24241 caccaccggg gtcagcgtct cggacgccga cgacatcacc ttgcggccat tttccggcga
    24301 gccggtaaag aaaatctttt cgacccccga attgaccagc gcccgcccgg tttccccgtc
    24361 ccccgtgatc acctgcacca gatccgggct atagcccgct ttggtcagca cttcgtcgaa
    24421 aatggcctga aagtcggcag ccgaccacgc ggtccactcc gatgtcttgg cgatgaccgc
    24481 attgcccgca aacagcgccg gaactgtcgg acagaaaatg ttgtggaagg gaaagttcca
    24541 cggacaaatc accccgacga cgccgagcgg gtgatattcg acccgcgccg ctttgtgcat
    24601 caaaagcccc gacggacgcg aatccgtttt tagatccgcc tcgccgtgct ccagcaggta
    24661 gttgatcttt tcgcagacgg ggaagatttc acccatcgcc gcatcggcca tggtcttgcc
    24721 cgaatcgacc gagcagagcc ggcagatgtc cgcttggtgc gcgacgatgt gatcgaggag
    24781 catccgcagc acacggcggc gctcagcaaa cgtcgtctgg ccccaggcaa tctgggcttg
    24841 gcgtgcccgc tcgacccgcg ctttgacatc cgcaggggat agcgacggca cctcgccgag
    24901 tttctctagc gtcgctgggt tttggcattc gattgtggcc aggggcaaag tccacttttg
    24961 gacttcatcg gtttgcttgg caagcatcgc ggctcctcgg gaaaacgttc gcacgcctta
    25021 tgccaaaaag ccggcaacat ggggtagcaa aaagcgcgat ccgccgcccg acccgccaca
    25081 gtcgcaacat gtccccgcaa aaggactacg gctgcgacgc caaaacggcc tgtccgaacg
    25141 cctcgccctg gccaacgctt gcccgccggg gctgtggctg gtcgccggac tggggaacgc
    25201 cgggctcgat gaccaaattg gcatcgtggg ccgcgccgcg accgggctct accaccacgt
    25261 cgagctggta gcgaacaaag ccaggcttta ccacttcgat ccggtgctcg ccagcaaacg
    25321 tcgtcaccgt cagcggagtc tgcccaagcc gcatgccatt gaggcgcacc gtcgcccccg
    25381 gtggcatcga cgtgatctcc agaagtccgg ttttgcgcat gtgggctttg tggacaagtt
    25441 ccccggtcag cgtcacaagc cgcgccgcaa gctgatcacc atcacacgac acgcaggtcg
    25501 tttcgaccgc cgaagcaaag tcggccatcg ccgcatcgta aatctgcccg ctcacttgat
    25561 agtcgtcgcc atcccgcacg atgcgcagcg aaaaaagctg ccgcagctcg gatttctgac
    25621 acagctcggc ttggcactca ctgtcgctgc agcggtcgag ctggggccag cgatcgcccg
    25681 gctgcgcggt tgacacatcg atgggatcgg cggtgacttt tttgccgcgc aggccgcgca
    25741 gaacgtgatg caaaagccgt ccccgcgacg cttcgtcttg ggatggtgcc gacagaagca
    25801 gcatcacctg aggcttgccc cccgacttgc ccgccgaatc atgcgaagct gacccatgcg
    25861 agccagcacg tccagcccct gggccaaaaa gcactgccga aagcagcgca cctagcaaga
    25921 tccccccgcg tcgcatcatg tgcagcccgg ctcccttatg acctgcttct ggcgggcgcg
    25981 cagctcgatg gtctgtgctg gactgccctt gatctgtagc tcgtgcgttc cgggccccag
    26041 ccagcgctct tcaggaacgc agcggccctt cttgttgtga ataatcgtca aaagcgcgag
    26101 cttgttttgc acccggcccg tcgcctcgga cagctctttg cgctgtgact tgctgagccc
    26161 gcgctgtgac ttgagcttct ggtattcgct catcgcctgc tcgtagcgcg cgtccccttc
    26221 gaagctgcgc ccgaggtaaa aacccagctc ccacgaaaac ttggcgcacg ctttctgggc
    26281 ttcacgcccg cacagcgcat tggcctgctg aagccgcgcg cgcgctttgt cgtagctgcc
    26341 atctttgaac gcgctcgccc cggcatgcac cagcttttcg atcgcctcta gctgcgctgg
    26401 cttgtcttcg accgcatcgg gtttttgcac agcctgcgtg gcggctgcgt ccggctgggc
    26461 agcgcccgca tcgggtctgg ctgccgcgtc ggtcggttca ggcggttttt ccgccgccgc
    26521 cttctgctgc tgatacaccg cggtcgcttt ggcccgcacc tcggggtcac ggtcacgcat
    26581 cagcgtgcgc aaaagcggcc ttgattcgcc ttcggtaagc cgcgccactt ccgccactgc
    26641 cgccagccga acctgcttgt ccggatcgcg cgccgctcgt tccagcagca gcgccgccgt
    26701 cgccgagtct tgttccaccg ccgcctgcac cgctgcggcg cgaactgccg gctcgctgct
    26761 atccaaaagc tcggtggcct ttttgggcgc ttcggcctgg acattgagcc gtcgcaaaag
    26821 cgcatacgcc aagatcccct cgggagagtc gggattgtcg gccaacgtct gccgaagcac
    26881 cgggatcgcc cgcttgtcgt ttagctcggc cagccgccgc gccgccaaaa atcggttctg
    26941 atcctccggg tcttccagcg ctgccgaaag cgtcgccgca tccttgtcca gcgattcgat
    27001 gacctggcgt cgcgcatccc catcccccga tcgcaacacc cccagcactt cggttcgctg
    27061 gctttcatca cccagcttca gcagtgtcgt tcgcgccagt gcctgctcac gcccgcttgg
    27121 acccgtcacc actttgccaa gccagccctt cacttcccca aggacatcct tctgcccttg
    27181 tgcaatcagg cgacggcccg cttcccccaa cgcgcggatg acttcctcac gcaccgacgc
    27241 atctttgtct tccaggccgc tcaccagtgc ggtcagcgca gcgcgatcac gcactttggc
    27301 cagcgcgcgc acagccccac ggcgcgtcgc cagatccgtg ctgtcgcgca aaacccgcgt
    27361 cagcgtaccg acctgtttgc cggtgccgac ttcacccagg atggcgagtg cttcctccgg
    27421 caagagtcct ccgccacctt gcagcgcccg ctcggccaag ctgatgtcct gcatcgccaa
    27481 gacctgcggg tccgcccccg tcagccgaat gatcgccgtt gctgccgctt gctgaagcgg
    27541 cggctcggtc ggaatgattc gcgcctgcaa aagctcgatg tcgggacgct gaccgctata
    27601 gccaagccct gtgaccgcca gcacctgcat gctcgccgtc gccgcgggcg cgttgagcac
    27661 attgcgaaac agtgccggat ccaccgattc ttccagctgc gcaagcagca ccgcggcgat
    27721 aagtccatcg agccccggct ttttgctctg ctcgcgcagg tactgtttcc ccgacggctc
    27781 gctgagcttg gccagcgaca tcgcaacttg ccgctgctgg tcgacattgc cgctgccgcc
    27841 aagccgaagc cgaagtcgtg accgcgcatt ttggtcgccg gcccgcgaaa gcagcggcaa
    27901 cacctccagc tgaccggttt ccgacaaaag accttgcgcc accgcctggg tcagcagctt
    27961 gcgcgcgtcc gcatcgccgg tcccacacag gtacagcgtc gcccgcagct tggcttgttg
    28021 atcgcccttg ctggccatcg tctttttcag cgcctgccgg ccccgcacgt cgccgagcag
    28081 atccagcgct tccgccgccg cagtttgcaa aagtggcgca accccgtccg cccccaaaag
    28141 cggtgccagt tgtgccgatg cctcacgtcg cccaagctga cccaaaatct cggcggcctt
    28201 gacctgcacc cgcagctcgc catcttttag cagcggcgac agcaccggca aaagcgtccc
    28261 atcgcgcgtt tccgcaagac cgctgagcgc ccccacccgc agctccaccg ctttggcggc
    28321 caggtcgttt tttagaacct cgaccgctcg ctgtcgtgct tcgagaagcc gcgtcgtggc
    28381 tgccaggctt tcatcatgcg cggcctgctt ctgacgcttg taaatcaccc agccgaccag
    28441 cgtgctcgcc cccaccacca gcagcgttcc ccaaatcgtc gccatcagcg cccacagcgg
    28501 ccagcccttc ttgaccactt ggatctgaac ttgcgactgc cgcccggtcg gcgacagcac
    28561 atcgatctcc gcttggagcg cttcctccag cgccttcatg gatggaaaac gcgccgctgg
    28621 ttctttcgcc agcgtccgca tcaccacccg ttccagcgat tcggggatct tcaggttcgc
    28681 cgcccgcgcg cccatcggcg gcaccggggc agacgcctgc gcgaacatca cggtcagaaa
    28741 gctggcgtcg tcaaacggca cgacgcccgt cagcatttcg tacatgatgc agccgagcgc
    28801 gtactggtcc gtgcgcgggt ccagctcgaa gccctgcgtc tgctcgggcg acatgtactg
    28861 cggcgtcccc agcaccatgc cgggcatcgt gtgccgctct ttcatcgcgc gctgtgcctc
    28921 ggcacccagc ttttctgccg acagcatgtc cagatcgacc tgcactgccg tcgcgcggtt
    28981 tgttgcgatg ccaaagtcga caatctttac aaagtctttt tgcccatcct ggtcgacgag
    29041 aaaaatgtta tccggcttga gatcgcgatg gatgatcccc ttgtcgtgga cggcctggag
    29101 tccgcgggcg atttgcaccg cgatccggca catccgcagc ggctccagcg ggccttggct
    29161 tatcaccttg ctcagcaccg ggccgcgcag aaactccatc gccaggaatg agcggtcatc
    29221 cggcagcacg ccgaagtccg acaccaccac ggtgttgggg tgcttgacct tgctcgccag
    29281 ctgggcttcc tgaagaaacc ggtactgcgc gtcctgatct tgcggtttta gcaagacctt
    29341 gatcgcgact tccgcatcga gaaggacgtg gcgagcgcgg tacaccacac ccatgccacc
    29401 ggcagaaagc cgctccagga tgtcaaagcg tccgtcgatg accgtaccga tgagatcatc
    29461 gttggaagaa tgcgtggtat ccggcgcact cggcggcggc acactcgggc tggagactgg
    29521 ctttacctcg tcctgactgc cacaggtgtg tttttcctct tcgggatata gctctccgca
    29581 gcgctcgcag cgacgtgctt gagcggtgtt gtgcacgctc atcggacgcc ttcccctggg
    29641 cgcgcaccgc agacgccaca tgcaccgtcc ccctgacggc gcgacgtgta ggcaaagttc
    29701 atgacgctag gccattttac acgagaaccg ggcgctgtgg ggttcggaat ttcgcaggat
    29761 tagcgacaca ttccgtcgca aagattggcg gtccacttgc gtctccacac aaaaaccgcg
    29821 tgacaatcgg gtagcatgta ggggccgtgg gcaccgacga gtcactcgat aaaaccgcac
    29881 aaccggatga agggccggac gcgacgctgg gtccggatga ccagcctgtc ccgacgctgc
    29941 gccttttgta tagccgcagc atcggcatcc tgacccccca gcgtgccaag ctgcgcagtg
    30001 aaccgagcgc ctggcggatt gacagccgcg agctacgaat tggccgcggt gcgcccccat
    30061 ccgaggtctg tctcgccgac gattcgcagc tatcgcgtct gcatgccagc gtcaaagcca
    30121 gcggcttaca ggtggtactc cgcgacctcg gcagctccaa tggctgtttc gtcgggggaa
    30181 ctcgcacaaa agaagccgtt ctccaagatg gcgacatcat ccgctgtggc ggctcgctgt
    30241 ttattttccg ctgcgaaccg accaaagttc ccgatgccaa gatcgattca ctgatcggtc
    30301 agagccttct gagccgtcgg ctgcgacatg agatcgccct gtttgcgccg acccagtgca
    30361 ccgtgctttt gctgggcgaa agtggcaccg gcaaagaagt caccgcgcgg gcgctgcaca
    30421 cgctgtcagg cgtcgcgggg ccacttatcg cggtcaactg cgccgcgatt cccgaaacgc
    30481 tggccgaaag tcagctcttt ggccacgtcg ctggcgcgtt caccggggcc aaagcccacg
    30541 ccggttactt tcgcgcggcc catcacggca ccctgtttct cgatgaagtc ggggaacttc
    30601 cggcctcgct tcagcccaag cttctgcgtg cgctggaaga aggggtcatc actccagtcg
    30661 gaagcgtcga gccccagccc tgcaaagtcc gactgatagc agccaccaac cgccacctgc
    30721 aaagcgaggt tgcagacgga aagcttcgcg gtgatctgta cgcccgtttg gtggaaatcg
    30781 tgttgcgcct gccaccgctg cgggcccggc gggaagaaat cctgctt
//
LOCUS       DAORGN010000036        30241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_563_length_30241_cov_5.158815, whole genome shotgun sequence.
ACCESSION   DAORGN010000036 DAORGN010000000
VERSION     DAORGN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 30241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30241
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_30241_cov_5.158815"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKL17_06295"
     CDS             <1..693
                     /locus_tag="PKL17_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNK44371.1"
                     /translation="AAGAHVVDFRPVAPTELLYLWMAVISSDAAATLQKQLEGEAVCK
                     QLQPTLQAAKLPDALKPLVARLLAVKGDERLAKLLRSLGRKPVEELWRLTAQRTQMRR
                     SELDAWNEANIDAVICPPHVLPAMPLGSSGDLTLTLSYMFRYVMLNFVAGVVPVTRVA
                     ENETTWQGPPPRDGVAKRCAEVVRGSAGLPIGVQVVSRPYREDVALAVMAAIEAAAVH
                     DPSFPKTPIDPA"
     gene            728..1438
                     /locus_tag="PKL17_06300"
     CDS             728..1438
                     /locus_tag="PKL17_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44372.1"
                     /translation="MQAESYDATQTKVDAALAAAEKRHAKNPERAELIARARRFKASW
                     IELAEALASCQKRQLYTEWGFVSFEEYYRKELHLKTATVNKLVGSYAFLRRAAPEVLE
                     RDGLSQPIPSIQSIEFLRRAEEAVESGQAPTDLLSEVRRAVLDDSLTLGKLNKQFKET
                     LFPSDADVEQKKRRKEARRLVQKLVDMLDLLRDSLPDSVIAQTEQALRELADMLPPEE
                     AGDEAAADAKPEEEAALQ"
     gene            complement(1495..2556)
                     /locus_tag="PKL17_06305"
     CDS             complement(1495..2556)
                     /locus_tag="PKL17_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNK44373.1"
                     /translation="MRNVAISVGVLLVALVIWVATRKPLSIDEELGKLPADERQALDA
                     LASAAGIAVAQLRPVGPGVLSYNPKAVAIQDGHVVELRIADASLKQIEPIAKLTALRT
                     LWLDGNPIASLQPISGLKSLRTLNLGRCKLGQVTELHDVPALTNLDLTGNQIDDVSSL
                     VALPALEKLVLSGNPLKSLPADAPKRWSVQSDLAPPAPPADSKPKTASQVATPPNWVD
                     ELPKPTGVKVRGTRGGRVTNAAYSVTGTIEKLDGVLEVGNIPGVAQISGGNTTLDLEV
                     QKGTVRAYLAKSIPDPSTLTGLKRGFIYAEATAGKPGQIVGDLRSFGPGFGKPRLYEF
                     VIESVGGEAEGISYKLSRQ"
     gene            2738..5236
                     /locus_tag="PKL17_06310"
     CDS             2738..5236
                     /locus_tag="PKL17_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44374.1"
                     /translation="MSDFEQAQTPSSTGSGYNHYGISGEQKNESGSYQNWTGWGSGVG
                     NRKDDTPQPDEKDANAPHSVKNSKMGAPSIDMVDLDGLRPEGVDPYDTNGVYRESLSG
                     VKPWENKWGEGKRDQEGKLSAELWSGSTVAGVREQTGIRGRAKTEGKYGTALAQGDAY
                     AVAEAGADAKAKLGTDGFEASTHASTRAGVGVNGDASLTSKDGRLASMQGIVDDELNM
                     GVGVHGDAFVGGKLGAGAKIGLGPEFIGAEAKVGGMLGAEANADIHGNLGPLKGKAGV
                     SGIAGIGFEASGGISIEDWKLHIGGKIGAALGLGGSVSVDATLDLKQTAQLAQWAGKQ
                     ICNGVQWAGQKVHDGLDRDGDGKLTLNDAAKGVGELAQGGANLIDKGWDGAQKLLDGD
                     KDGKFSFNDVKIRADQAKDWLASKGSQALGWAGDQVNAAGKALHNMADRDGDGKIGVG
                     DLVAGAAQAKEFAKEKIGQAGQALGNAKDWAVDKAKQAGTWLKKKADLDGDGKVGIGD
                     VFTGIGEVKNFAGEKIQQIGGAIGAAKDWAVDKAGDAKDWLLEKAANAGSAIKNAADR
                     DGDGKVGFSDLVTGLGQAKEFVGEKAGAAGKAIGNALGAAKDWAGDKLHSAKDAIHNA
                     ADRDGDGKVDWHDAVAGAGQLKNWAGDKLSSAGKTIGNAVGAAKDWASDKLHAAKDTI
                     HNAADRDGDGKVDWHDAVAGAGQLKNWAGDKVSAAGKTIGNAVGAAKDWAGDKVASAK
                     KAVTQAADRDGDGKIGWGDVKAGASQAKEWAGDKISSAGKAIGAAKDWVGNKASAAWD
                     GAKSAGSTLVSGVSGAAKTVGGTLKKAKDFFGGW"
     gene            complement(5304..6368)
                     /locus_tag="PKL17_06315"
     CDS             complement(5304..6368)
                     /locus_tag="PKL17_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNK44375.1"
                     /translation="MRNVAISVGVLALVLVIWVATRKPVSLDDELATLPPEERQALTA
                     LVSAAGLSPAQLRAVAPGVLTYNPKAVAIQSGHVVELRLADTPLTKADDVAKLPALRT
                     LWLDGAKLETLRPLSGLKSLKILNLSRCQLRSVTDLVDLPALTSLDLSGNQIEDVSAL
                     VGLPALEQLTLSGNPIQRLPSSAPSRWKVKSDMTDAAAAQKSEPALEKPDNWIEKDKA
                     PKTSGTAKPGRVTGVVTNAEWSVKGDISVLQGTVVIDGIPGDSNINGTDAALEVEVES
                     GQVRGYIEKALRIPGSTFKRRHGYAFCDASPGKPGRVSGSLTSGGPGNYGVSQSYHVI
                     FESIGGEAKGIKFRLMPPSR"
     gene            6506..8806
                     /locus_tag="PKL17_06320"
     CDS             6506..8806
                     /locus_tag="PKL17_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44376.1"
                     /translation="MSDFEQEQGDAHSGGSAFDGLSAPVNHYDISGTEKSQGGYTPSY
                     QSWDAWGYGAGNRKNDTPQVDPKKPDAPGTVKNSTMGAPSVDQVNLSDLKVPADQRGP
                     ANGFYDESVAGTVPWKEKWGSGKYNKDGTSADLWSGSTVAGARRQAGLRGGVSVEGDY
                     GKAEASGDLYANAEAGVGANAKVTNTGVEAGAQASARAGIGVRGDANLTSADGRLASM
                     DGIVTDPLNAGVGAHADGFAGVKVGAGVKAGIGEKFTGIEGKIGGFAGAEGSADIHAN
                     LGPLKGKAGVSGIAGIGFEASGGISLEDWKLHIGGKLGAALGLGGSVSFDATLDLKQT
                     AQLAMWAGKQIHNGLDRDGDGKLSLNDAAKGVGELAQGGANLLDKGWDGAQKLLDGDK
                     DGKFTFNDVKIRAGQAKDWLASKGSQALGWAGDQVNAAGKALHNMADRDGDGKIGIGD
                     LVAGAAQAKEFAKDKIGQAGQALGNAKDWALDKAKQAGTWLKKSADRDGDGKLGIGDV
                     LTGIGEVKNFAGEKMHQLGGALGAAKDWAADKAGDAKDWLLDKAANAGKALHNAADRD
                     GDGKLGFSDIVAGMGQAKDWASDKASAAGKAIGNAAGAAKDWIGDKASAAGKALHNAA
                     DRDGDGKLGWGDVVAGAGQAKNWLGDKAASAGKALGAAKDWASDKASAAGKALHNAAD
                     RDGDGKLGWGDVVAGAGQAKEFAGNAVNTAGKALGAAKDWASDKASAAWDGAKSAGKT
                     LVGGLSSAASTVGGTFKKAKDFFGGW"
     gene            complement(8866..10974)
                     /gene="uvrB"
                     /locus_tag="PKL17_06325"
     CDS             complement(8866..10974)
                     /gene="uvrB"
                     /locus_tag="PKL17_06325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNK44377.1"
                     /translation="MSQGTPLQIVSQMEPKGDQPTAITQLVEGIQRGDKRQVLLGLTG
                     SGKTFTVACVIEKVQKPTLIIAHNKTLAAQLYGEFKELFPNNAVEYFVSYYDYYQPEA
                     YIPTSDTFIEKDSLINEEIDRMRHSAMHAVVSRRDVIVVASVSCIYGIGDPEFYRSMV
                     LSLDVGQSVRRDAVLRRLVELQYERNDIDFHRGTFRVRGDTVEVFPPFEEEVAIRIEW
                     FGDDIDSIAEVDPLRGKTLRKLKEVSIFPGSQYVTPADALVSAMRAIKEELREQITNF
                     KDNMKLVEAQRIEQRTLFDLEMLDQMGHCSGIENYSRHLSGRAAGEPPATLIDYFPRD
                     FLCIIDESHQTVPQVQAMYRGDRSRKETLVSYGFRLPSALDNRPLKFEEFNHRVPQAV
                     FVSATPGDYELEQTGGVVVQQVIRPTGLLDPQIEVRPVSTQVDDSISEIRLRIAQGDR
                     VLVTTLTKRMAEQLTEFYAENGIRVRYLHSDIDTLERSEIIRDLRQGVFDVLVGINLL
                     REGLDLPEVSLVLILDADKEGFLRAERSLMQTCGRAARNEQGRVIMYADRITRSMQVC
                     LDETARRRKIQDEHNKKHGITPQTVKRKVSDLRAIVQGELTHAAEDLAAGDARPGYGS
                     DKDKERKGRPDKKTGLPFGAKKQAQSKDEAPLDLANIPALLTKLKAQMKEAAADLNFE
                     RAASLRDRIRELEQLDLMLR"
     gene            complement(10981..13782)
                     /locus_tag="PKL17_06330"
     CDS             complement(10981..13782)
                     /locus_tag="PKL17_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK44378.1"
                     /translation="MKRGRGLWAALLLVVLLLVPRGLWAIGEVTGRLGGVVTIDGTKD
                     GLSGVPIVVRSKQLLGGSRTIETGDDGSYLFQSLPPGTYELEAKIEGFAPVEQRGIVV
                     VAGQLAAVDVVLHVGQITETKKIIEKRNPILNPESAVSTTTLDNQKVSRTPVFRQVQS
                     IAQLAAGVGPGTAPSVRGGLSRYTRFLVDGLDTSDVVTGGLSSPMNFDAVEQYNLFTG
                     AMDAEYNSLGLVQNMVTRSGGNNFTLDASVVIQPTAFNLRTRYGAQLPQQNQALIYDD
                     RDPPVRDFYSVNANVGGPIVKDRLWFFTSFQFNYNKAMTTVPPFPWLGITEDTDRFRD
                     TYTYLGRAKLTWQVTTGTRVSLSFNIDRNYITNSAQQNTLAQEAERRVGRGGEWLVLL
                     IDSALSPKWMFQMQAGFTDKRSVEDTMAVGPDGLPDRITPSHVLRTSDQFNGVTYLNS
                     SLGWNEETKYRVQVDPTFLYSSMGLGGMHNVKFGAQFAYMSYRHNVGIAGGRNYVDTV
                     PGTPCDPADPNTHASCSQVTEYPDSLPAVDGSAAGGFSTTAEAFNIGFFAQDRYTIKR
                     YLTLVPGFRFDVGVLRDYQGNKLGTLVGYGPRLSLVYDLLHDRSTLISAHYGRHNDVG
                     NTYIADRGNPQQVAIQKNWDNASRSFVERNRSGGPGGQLFADNILPPSLDEVAAGIRR
                     EIITELIVGVDYTFRRYANMWANSEVNQVWDPAGTRIVGYVNGERRKIYQAETPDDAQ
                     RTYHGLDLWAQGNPGNWSLVASYTLAFTDGTVSDYFSAYRQNPRLNPLFYGPMSDNYR
                     HTLKGAVDYSFDFGLNTSVRLQYRTGAAQWKVFQSPEDTSFSLYRSPRGTSTGTRVND
                     PTTWANFQLPDQFTVDLQFTYNFQKLTGQRIDLMLFFFNVLGAGIPTSIDARNGATFG
                     TIQGRADNFNAEIIVRYRF"
     gene            complement(13798..15228)
                     /locus_tag="PKL17_06335"
     CDS             complement(13798..15228)
                     /locus_tag="PKL17_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44379.1"
                     /translation="MSQKSTLVRSVGLSLLPVCLLSLSAACSGVIDPDPYTGTIDPSA
                     FDKQYKFQPASREDPVNGCLLPRRGWDGASLSDSPRFYYLGALFAAQLDLAGAFDTTR
                     ALPASVYSLSGCNAPEGRKDPGEFDIRVNNYDRRVQYPILSQGLFPVQVGSAGEPTAQ
                     ASYKPFHLVVQVQLETTVRDRMGCNDVKRELSLLERAGWDRATKTFPDTGPKDFDFAF
                     PSREQIRSGAVRFKDWPMVNVGVPVGRSLDPIQACPFVTGSKAVYPQNLSDSAAGFQF
                     PSQAWFRGLLSGYVDGGDLPLITEARKCPALFDSGKSCKVDAMGMVTGCSVADGEVCT
                     ATDPAKSGTCLVPPPVCPVINELYVSKDEFDPPSASSFDSSDPLPRSKSIVTLTDAMD
                     MTKTRKADALAMFAVSPGQPNYSPVCRVRWVDLSRATCAAKENQTIKPRPLCTIAELQ
                     ASTGAILPTAPGKDVFVHCLSLAAAK"
     gene            complement(15338..15724)
                     /locus_tag="PKL17_06340"
     CDS             complement(15338..15724)
                     /locus_tag="PKL17_06340"
                     /inference="COORDINATES: protein motif:HMM:NF023903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HNK44380.1"
                     /translation="MSKARIPCKIPDRVLQAFEQELAKARQAETRQQFDEAWRHLERA
                     HVLGQAWNSPHARVHLGMMRWSLRRGDLSRLVRQTLILLSNPLATALVRGLVGASGQN
                     VNPLRRGPVPSDLAEILRLGVKDATA"
     gene            complement(15804..16910)
                     /locus_tag="PKL17_06345"
     CDS             complement(15804..16910)
                     /locus_tag="PKL17_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNK44381.1"
                     /translation="MQDSSGDFLARISSFGDPWRVTWRRRLVTIPLFYLVGTLIVVLL
                     PLLALAGLVTAAFERHPFARLRFIFFLAGYFGGEMLFLFLLFLQWVFLLGFLPGSQVR
                     LAHAAESLSNAWGRVLYQLGVWSFDLRLSFDGLEQLEKPGPLLVLLRHASLGDTPLAP
                     TYLGSRYGFRLRYIVKRELINDPVFDVIGGRLGSCFVRRGSKNSGREIESVCAQLEHL
                     SPRDAVILYPEGTRYTDAKRLQVMAKLERDNPALYARVSQLSHVMPPQLGGAVELLCR
                     NPSADVLIVEHVGYEVAATFGDLVDGRALHQRIEVRFRRIPFAEVPKDREEIISWLLE
                     QWTQMDQWVGQRKPHVITKSAPDLPALTLGSSAK"
     gene            complement(17380..19287)
                     /locus_tag="PKL17_06350"
     CDS             complement(17380..19287)
                     /locus_tag="PKL17_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNK44382.1"
                     /translation="MNSLTRLFSSSLLAIAVLPSASAFASAPLAPKAAACLTESVKNT
                     AKNDPQIRASASRGLAFLSKASQAWTQQHRCFGCHVQAVTMEALSVGKKHQYTIAGQD
                     LAEMVKALKMGVTAGGHTTGIAFEGSAWARYDEWVGNNETAELLRYAQELLSVQGKDG
                     AINDDDRRLPVTGGTLETTFQAAQTWRQAYARTAEQKWLSPMRRAEAYLAKHSASFGS
                     GKQVYVQDINFSLLGLLASGVTRNEASAHNLIKKLLDRQNGDGGWGLDPQKSDAFATG
                     QTVYALKLAGYSDADTQVARGIRFLVSTQDQSGAWRTYRSGQGGAEKAETMWAVLGLV
                     TVDVASLKVTGLTDGQHVEPKMTLAVSAADNQGAGIKQIEVLVDDRSVHTECGAKLAY
                     TLDTAQLSAGKHMIDFVAVTDKGQRSVRRYTVYAGDVFMTELGAQFDEKSSSSVVSLR
                     NIAPQSEQSGSVEMEVYTIKDGGKDAGKDTKTSEPKDRVFVSSKKGELGGLSFSWSGQ
                     GSDGKALPVGRYVAKVQFRDAQGKVRQTDSTIFFHGSEAAQRAKFAEVEGQIALRGGS
                     GSANTLVELVDDSGNVVQSTRTTEQGNYRFKSVDRGQYKVRVRKNGFSDLEQNIQAAP
                     AAAPAKASMSW"
     gene            complement(19392..21545)
                     /locus_tag="PKL17_06355"
     CDS             complement(19392..21545)
                     /locus_tag="PKL17_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNK44383.1"
                     /translation="MHALPLLLATKLVVWHAYAGGEEKALTEVVERYQKQHPQGPQVE
                     AVSVPYGSFADKLQAAIPRGHGPDLFIQAHDIVGQWSKLGLIEPLNSHLPLADVLPTL
                     WPETIPPLKDGDALLGLPLSWKTLALFYRKDLVATPPRTTADILQIGAEQKAHSPAGS
                     RRYGLVYEAGNFFKHAALLHGFGGAIIPPGSPVPTLDTKEQLAALQFVSQLVQNGDLP
                     EEMSAVLTTQFFNEGRAAMTINGPWFIAEIAKDVPYGVAPMPVVSETGQIAAPLTSIE
                     AGFVSAKSEHKGEAADFLRYLASAESAQIRATVGRQSVSEKTTWDRPEVQNDPVLSAF
                     FAQRQHIVPSPTHPAMRAFWEPGEQALRQVLRGGKPQDALRAAQRLLDQYLRPPPATA
                     DDRPYLLALSVVVLGLCVWQYLRLRRRDVFGEAWRERDAYGYIGPALFAMLLLSLIPF
                     AVGAGMSLFHRDLDGSWRFVGAENFADILLCRGGGCLEPMSFYFTLLVTICWTLANVG
                     LHLVIGGGLALLLRSPWLALRGVYRMLLIVPWAVPNYITGLIWKGMFNRQFGAINGLL
                     QLLGIAPVSWFSHFSTALAANIATNVWLGFPFMMVVALGHLSQIPSELEEAAMLDGAN
                     LWQRLRHVVLPLLLPAMLPSVILGAVWTFNMFNVVFLVSGGEPDGATDILVSQAYRWA
                     FTRGHRYGFAAAYAVLIFVILIIQSALTRRLTDRR"
     gene            complement(21545..22663)
                     /locus_tag="PKL17_06360"
     CDS             complement(21545..22663)
                     /locus_tag="PKL17_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK44384.1"
                     /translation="MANVSIRRLCKDKPSALGKGGPHRILHDISMEIADGEFLVLVGP
                     SGCGKTTLLRCIAGLEEPSSGELAIGDHVVNDVPPQARDIAMVFQSYALYPHMTVAEN
                     LSFGLKMRRTAAPEIKKLVEEAAQMLEISHLLSRYPRELSGGQRQRVAMGRAVVRRPK
                     AFLFDEPLSNLDAALRQQMRLELLRLHRRLKATMIYVTHDQVEAMTLADRIAVLRQGR
                     IEQMGTPAQLYDNPANTFVARFFGTPEMNLIDGTIEAGWFSGDGIPFRVHVPGFADQP
                     VTLGVRAEDLHLHKADGTELPRGRIDVVEHHGHEDLLHVRCGDKVLSVRSIAHKSPPP
                     GTDVHLVVSPENCHLFQRRDDKGDGNRLSPYEKSEAAA"
     gene            complement(22736..23218)
                     /locus_tag="PKL17_06365"
     CDS             complement(22736..23218)
                     /locus_tag="PKL17_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44385.1"
                     /translation="MFDFDLPDPGLYRTTQPMPGNESAIPAGVLVFIGQPKTGGVKFV
                     VRPGQNRRNRWFWQDPTTPLKATHAAWARTLKALPSEGFYVLPETINMDGGGKWLKNA
                     IVELGYNEEGKGIIFVGQWQEDGEDNSLFFSDRGMMVDDRLLAKLIWAPILPVNGKPQ
                     "
     gene            23451..25256
                     /locus_tag="PKL17_06370"
     CDS             23451..25256
                     /locus_tag="PKL17_06370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44386.1"
                     /translation="MVGRTLAHYRLLELVGHGGMASVFRAHDEQLDREVAIKVMHPHL
                     ATEAQARQRFSREARAVARLRHENILEIYAASDPESPESYLVTEFVSGPTLRKFFELH
                     GPLPAEIVGLLGWVLADALAAAHEFGIVHRDVKPENVMIRTDGRIVLCDFGIARLLDN
                     DSVTNTGQLLGSPAYIAPEHISGQPQDGRSDLFALGVLLYEALSAELPFSGRNPHETL
                     TLIARGEHKPIGERVPGLPRPLEQIVEKLLSCSPDDRYPTARNLGLALLDFLRDVGLA
                     TPREQLRSYFLDPEGFWQSFLPQLVETLIKNGKARAEEGRTAAALSDWGRVLLLSPGH
                     AEAEELIARASRLKQRRRIVRTIWISIGAAAACVGLAVLGQSVLSRPKVAPPLHIVAA
                     QQTAASAVPISADTDKAPVISVDDPSPVPAETDLGQDASTSKPKLVHRPLPVKSAAPT
                     HVVRLEPWPKSVTVTHNGKRLGAYGTDVRTVQLGPGPNEFLFESQACYSEKITLPAGS
                     TPEEVRVRLRWKPALLQVRAHSGRLQPSDPVDILVDGHIVGRGGQVMAIPVSADEGHQ
                     AVSVQVGAPGHKTHTENVQVRANQLLQLDVDLPMR"
     gene            25321..26523
                     /locus_tag="PKL17_06375"
     CDS             25321..26523
                     /locus_tag="PKL17_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNK44387.1"
                     /translation="MKRLWGLLVCMSLLFAAGCSGLYMNIPMTRPASVDISGIRKIAV
                     LGLPQNPDTPPSFGSELTGRLAANLTSNGYFTVVERPDLMQLLAQLQMPPEALNDPVG
                     LSRIGQGLGVDAIIIGSVDSLRVQDTTGVDLLDVRIPVIAPVVAVAPPMGGTVVLPPP
                     PTPPPVVVAPPPRHRHRTTVTPPPPPPPPAAVEPGYVIERRPFPFVVRTANLVMKLQL
                     VRVKDSRLIHMLPVSFTASGKFYPGPQPDPAPSYHSGPALSTSPYVMRSLPAPEAMVS
                     LFASEMSGTFASHIVPTRYTKRIEFASDKNPDNQRGLEFCRSNAWNLAYQAFTEGLAK
                     DPNNAALHYNLGILRESDGDYATANAEYQRALSIDPSKKLFQQTYRRLQDSMAEQEVV
                     RRQMEAQP"
     gene            complement(26546..27526)
                     /locus_tag="PKL17_06380"
     CDS             complement(26546..27526)
                     /locus_tag="PKL17_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44388.1"
                     /translation="MSMSRVGVAVRMLMLLLTGLSLGGCASVYVSWMQPRADGTAEFV
                     LFSKSRSLGLRKSGGEFFVADEYTPAERVLWKEGAPSRFVGKSNDTLSPPAGLVPRYI
                     VTVALCSPPESLPASDARQREGDPLVWCLWKDEATQTVRVEARLPFVDPPRFRQVFQG
                     PDLPASVEPLSGQRAVMTRGGRLFLLDAQSGTVQLVLREPAELRGATADGDLLVSVQG
                     GPNMLLLRSHKVLPLPTTFSMGGLAAAAGALWMTNLYEHRLFRIDPETATLTEVVRPV
                     GCVKLLGGDVLGHLWFSTEPSEVRRGHALSLCRWDPKSPSPVVVQIPTEL"
     gene            27645..30086
                     /locus_tag="PKL17_06385"
     CDS             27645..30086
                     /locus_tag="PKL17_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44389.1"
                     /translation="MISLLALSATIASGSHFTCAIGSGGAVFCFGANESGQLGDGSRE
                     SRGKPVRVVGLTGAKDIAAGDTHACVVDAEGHVLCWGNNGPGSCGVPLDIHVTTPQRV
                     EGLPNASRVFAGHSTSCAVSTDGRLFCWGRNLSNQLGVPRGPHEAGRPSPVEIKELKD
                     VQSVAIGDSHTCALTKRGEVYCFGSNEHGRSGGQGSVVPPTRINIGPAVAIAATSNAS
                     FATLKDGTLYFWGSWQRDRDFVMAPVPTPTGDRNVTLLFTNRHSAYVRSGSNDWRFPK
                     AGVAAPMPPTGLSWAVEVSAGETHACGRDRDGALRCFGTNREGIGTGVPWNGKEPVVV
                     PLDAPESDPLGTTAQPPPPPAACEASAASPSWQQAKPTPPTCGNGKRDVIGMAGGQCP
                     PCIPDRECHCAPRVELREACDGKDLGGASCSSLGYGSGKLACTAQCTVDTSGCVPASH
                     LPSGARLAFVELPIDKTPTAGMAIAPNDNVLGAVWGATGQCGQAVFGQFSAGLKLLST
                     SAPFGRPHASRMQLVATPSGWLAAVGDRLNTWVHLIDASGQPGPEQVAFRGRPVFLQS
                     QGPNGPYLLGLSTRNHPYMGGLDTVLLSGSGEVLAKSTVFGHQSSASETMDSYPPPAD
                     QVAITTLDDGFAVARAQLVPGVMDLGGIVVARLAKDGQVQAKSLIAIGGTSPFFGSHK
                     LLGWLSNLSGPGETQRYGVQTVSIDANGAAQGEPQLVTKLDPSEWIITGALAGPSPVL
                     LVPQVDAFQSTFQSNTARRIDLLTLPKQRLTVIDGGGMGAAKLLRWNDAMVAGFVWQR
                     DGRARIGLMTAPAAK"
BASE COUNT         6189 a         9485 c         9185 g         5382 t
ORIGIN      
        1 gcagccggtg cccacgtcgt cgattttcgg cccgtcgcgc ccacggaatt gctctatctg
       61 tggatggcag tgatcagcag cgacgcggcg gcgacgttac aaaaacagct tgagggtgaa
      121 gcggtctgta agcagcttca gccgacgctg caagccgcca aactgcccga tgcgctcaag
      181 ccactggtcg cgcggctgct tgcggtcaaa ggcgacgagc ggctggcgaa acttttgcgc
      241 tcgctcgggc gcaagccagt ggaagagctg tggcgtctga ccgcgcagcg cacccagatg
      301 cgacgcagcg agctggacgc ctggaacgaa gccaacatcg acgcggtgat ctgcccgccc
      361 catgtgctgc cggccatgcc gctcggttca tctggggatc tgacgctgac gctgtcttac
      421 atgttccgct atgtgatgct gaactttgtc gccggagtcg tgccggtcac gcgcgtcgcc
      481 gaaaacgaaa ccacttggca aggcccgccg cctcgcgatg gcgttgccaa gcgctgcgct
      541 gaagtggtcc gcggcagcgc cggactgccc atcggcgtgc aggtcgtctc ccgtccctat
      601 cgcgaagatg tcgcactcgc ggtaatggcc gccatcgaag cggcggcagt tcacgatcca
      661 agcttcccca aaacccccat cgaccccgcg taatcgcttg ggcctggccg tgctataaac
      721 cgcggccatg caagccgagt cttacgacgc cacccagacc aaagtcgacg ccgccctcgc
      781 ggcagccgaa aaacgtcacg ccaaaaatcc tgaacgcgcc gagcttatcg cccgcgcccg
      841 ccgcttcaaa gcaagctgga tcgagctggc cgaagccctt gcgagctgtc aaaaacgcca
      901 gctttacacc gagtggggct ttgtgtcctt cgaggagtac tatcgcaaag aactccactt
      961 gaagaccgcc acggtcaaca agctggtcgg ttcgtatgca tttttgcgtc gcgcagcgcc
     1021 cgaagtgctg gaacgcgatg gactcagcca gcccatccca agcattcaga gcatcgagtt
     1081 tctgcgccgc gccgaagagg cggtcgaaag tggccaagcg cccaccgatc tgctgtccga
     1141 agtgcgccgc gccgtgctcg atgacagcct gacgctcggc aagctgaaca agcagttcaa
     1201 agagacgctg ttcccatcgg acgccgatgt cgagcaaaag aaacggcgca aggaagcgcg
     1261 ccggctggtg caaaagctgg tcgatatgct ggatctgctg cgcgacagcc tgcccgattc
     1321 ggtgattgcc cagaccgaac aagcgctgcg cgagctggcc gacatgctgc cgccagagga
     1381 agcgggagac gaagcggctg cggatgcaaa accagaagaa gaagcggcgc ttcagtaacc
     1441 aagcaaaaca aaggccggtg gttgcccttg gatcaccgac cgcgggccgc cagattactg
     1501 ccgcgacagc ttgtacgaaa tgccttcggc ttcgccgccc acgctctcga tcacgaattc
     1561 ataaagtcgt ggttttccaa aacccggtcc aaagctgcgc aaatctccca caatctggcc
     1621 cggcttccct gctgtcgctt cggcatagat gaagccgcgc ttcagtccgg tcagcgtgct
     1681 cggatccggg atcgacttgg ccagatacgc ccgcacggtg cccttctgca cctctagatc
     1741 gagcgtcgta ttgccgccgc ttatctgcgc aacgccggga atgttgccaa cttccagcac
     1801 accgtccagc ttctcgatgg ttccggtgac cgagtaggcc gcgttggtga ctcgtccgcc
     1861 acgggtgcca cgaactttga cacctgtggg cttgggtagc tcgtcgaccc aatttggcgg
     1921 agtggcgacc tgcgatgcgg tcttgggctt gctatccgcc ggtggggccg ggggagccaa
     1981 gtccgattgc acgctccagc gcttcggcgc atctgccggc agcgacttca gcgggttgcc
     2041 cgatagcacc agcttttcca gcgcgggcag cgcgaccaaa gacgacacat cgtcgatctg
     2101 atttccggtc agatccaggt tggtgagcgc cggcacatca tgcagctccg tcacctgtcc
     2161 aagcttgcag cgacccaggt tcagggtccg cagggacttg agcccgctta tcggctgaag
     2221 cgacgcgatc gggttgccat cgagccacaa ggtccggagc gccgtcagtt tggctatcgg
     2281 ttcgatttgt ttgagcgacg catcggcgat ccgcagctcg accacatggc cgtcttgaat
     2341 ggccaccgct ttggggttgt aagacagcac acccggcccg accggtcgga gctgagccac
     2401 cgcaatccca gccgccgatg caagcgcatc gagcgcctgt cgctcatcgg cgggcagctt
     2461 cccaagctct tcatcaatgg agagaggttt gcgcgttgcc acccaaatca ccaaggcgac
     2521 gagaagaacc ccgacggaaa tcgcgacatt tctcatggct tgtagtctat cattggaacg
     2581 gggcgatgcg gagagacgct gtctcggtcc gcgcggcttt ggccggtgtg ggccaaggta
     2641 gaccgctgtc gacagcgaca cgcgcggcgc gtctgtcagg caacttggcg cgtcgcgtgc
     2701 cgaaaatttg gtcacagaag ttagttgggg gatcgacgtg agcgactttg agcaggcaca
     2761 aacgccttct tccaccggtt cggggtacaa ccactacgga atctccggcg agcaaaagaa
     2821 cgaaagcggc tcgtatcaga actggacggg ctggggctca ggcgtcggca atcgcaaaga
     2881 cgacaccccg cagccagacg aaaaagacgc gaacgcaccg cacagcgtca aaaactccaa
     2941 aatgggcgcg cccagcatcg acatggtcga tctggacgga ctgcggcccg agggagtcga
     3001 cccatatgac acaaatggtg tctaccgcga gtcgctctca ggcgtaaagc cgtgggaaaa
     3061 caagtggggc gaaggcaagc gcgaccaaga aggaaagctg agcgccgagc tgtggagcgg
     3121 cagcaccgtc gccggcgtcc gtgaacagac cggcattcga ggccgcgcca agacggaagg
     3181 caagtacggc acagcgctcg cgcaagggga cgcatacgcc gttgcagaag ccggtgcgga
     3241 cgccaaagcc aagctaggca ccgatggttt cgaagccagc acgcatgcca gcacccgcgc
     3301 cggcgtcggc gtaaatggcg atgcgtcttt gaccagcaag gatggccgac tggcatcgat
     3361 gcaaggcatc gtggacgacg agctcaacat gggcgtcggt gtccatggcg atgcgttcgt
     3421 cggtggcaag ctcggtgcgg gcgcgaaaat tggcctgggc cccgagttca tcggtgctga
     3481 agccaaagtc ggcggcatgc tcggtgcgga agccaatgct gatatccacg gcaacctcgg
     3541 ccccctcaaa ggcaaggcgg gcgtttcggg tatcgccggc attggctttg aggcatcggg
     3601 cggtatctcg atcgaagact ggaagctgca catcggcggc aagatcggcg ccgcactggg
     3661 cctcggtggc tcggtgtcgg tggacgcgac gctggatctg aagcagaccg cccagctcgc
     3721 gcagtgggcc ggcaagcaaa tctgcaacgg tgtccagtgg gcaggacaaa aagtccacga
     3781 cggactcgat cgcgatggcg atggcaagtt gacgctcaat gatgcggcca agggcgtcgg
     3841 tgagctggcc caaggcggcg ccaacctgat cgacaaaggc tgggacggcg cccagaagct
     3901 gctcgacggc gacaaggacg gcaagttcag cttcaacgat gtcaaaatcc gcgccgacca
     3961 ggccaaggac tggctcgcaa gcaaaggcag ccaagcgctc ggttgggcag gcgatcaggt
     4021 aaacgccgcc ggcaaggccc tgcacaacat ggccgaccgc gatggcgacg gcaagatcgg
     4081 tgtcggtgac cttgtcgccg gtgcagccca agccaaagag tttgccaaag aaaaaattgg
     4141 ccaagcaggt caggcacttg gcaatgccaa ggactgggcg gtcgacaaag cgaaacaggc
     4201 cggtacgtgg ctcaagaaga aggccgatct ggacggcgac ggcaaggtgg gaatcggcga
     4261 cgtgttcacc ggcatcggcg aggtgaaaaa cttcgcgggt gaaaagatcc agcagatcgg
     4321 cggtgcgatt ggtgccgcca aagactgggc cgtggacaag gcgggcgacg ccaaagactg
     4381 gctgctcgaa aaggcggcca atgcgggcag cgcgatcaaa aacgctgccg accgcgatgg
     4441 cgatggcaaa gtcgggttca gtgaccttgt cactggactt ggccaagcca aagagtttgt
     4501 gggcgaaaaa gcaggcgcgg caggcaaagc cattggcaat gcgctcggcg cggcaaaaga
     4561 ctgggcaggc gacaagctcc actcggccaa agacgccatc cacaacgccg ctgatcgcga
     4621 tggcgatggc aaggtcgatt ggcacgatgc ggtggctggc gcaggtcagc tcaagaactg
     4681 ggcaggcgac aagctcagct cggcgggcaa gaccattggc aacgccgttg gtgccgcaaa
     4741 agactgggcc agcgacaagc ttcacgcggc caaagacacc attcacaacg ccgctgatcg
     4801 cgatggcgat ggcaaggtcg attggcacga tgcggtggct ggtgccggtc agctcaagaa
     4861 ctgggcgggc gacaaggtaa gcgctgccgg caagaccatt ggcaacgctg tcggcgccgc
     4921 aaaagactgg gcgggtgaca aggtggcgtc agccaaaaag gcagtcaccc aagccgccga
     4981 ccgcgatggc gacggcaaga tcggctgggg cgacgtcaaa gcgggcgcaa gccaagccaa
     5041 agagtgggcg ggcgacaaaa tcagctctgc cggcaaagcg atcggggccg ccaaagactg
     5101 ggtgggcaac aaagcctccg cggcctggga cggagccaag agcgccggca gtacgctcgt
     5161 cagcggcgtc tctggtgcag ccaagaccgt cggcggtacg ctcaagaaag ccaaagactt
     5221 cttcggcggc tggtaacttc cgcttcgccg tggctcgccg ggcccatccg gcgggcccgc
     5281 ttcaaaaatc ccctcgaaac agcttaacgg gacggcggca tcagccggaa cttgatccct
     5341 ttggcctcgc ccccgatgct ctcaaagatg acgtgatagc tctgggacac gccgtagttg
     5401 cccggtccac cgctggtcag cgatccggac actctgccag gcttgccagg gctggcatca
     5461 cagaacgcat agccgtggcg acgcttgaac gtgctgcccg gaatgcgtag tgccttttcg
     5521 atgtagccgc ggacctgacc actttcgact tcgacttcca aggccgcgtc ggtgccgttg
     5581 atgttgctgt caccaggaat cccgtcgatg acgaccgttc cttgcagcac gctgatgtca
     5641 ccttttaccg accactcggc attggtcacg acgccggtca ctcgacctgg cttggcggtg
     5701 ccgctggtct ttggtgcttt gtctttttcg atccagttgt cgggcttttc caaagcaggc
     5761 tccgattttt gtgccgctgc agcgtctgtc atgtccgact tgaccttcca gcgggacggc
     5821 gcgctgcttg gcaaacgctg aatcggattt cccgaaagcg taagctgttc aagtgccggc
     5881 agccccacga gcgccgatac atcctcgatc tgattcccgc tcaaatccag gctcgtcagc
     5941 gcgggcaggt cgaccagatc ggtgacactg cgaagctgac agcggctcaa gttcaggatc
     6001 tttagcgact tgagtcccga cagcggtcgc agcgtttcga gcttggcgcc atcgagccac
     6061 aacgtgcgca gcgcgggcag cttggccacg tcatccgctt tggtaagtgg cgtgtccgct
     6121 agtcgcagct ccaccacatg gccgctctga atcgcaacgg ccttcggatt gtaggtcagc
     6181 acgcccggcg cgacggcccg aagctgagcc ggcgacagcc cagccgccga aaccagcgcg
     6241 gtcagggctt gccgttcctc cggtggcagg gtcgcaagct catcgtctaa cgacacaggc
     6301 ttgcgagtcg caacccagat gacgagcacc agcgcaagaa caccgacgga aatcgccacg
     6361 tttctcatgc caacagtcta ccacctgatg gatcggccat ggctctgctc atgccgcaaa
     6421 aaacgcgcgc cgttctgccc gcgctgccca cacaacttcg gtgcgcagac gccgacaacc
     6481 caaacaaagc agttggggga atctcgtgag cgacttcgag caagaacagg gcgatgctca
     6541 ttccggcggg tctgcatttg atggactgtc ggcaccggta aaccactacg acatctccgg
     6601 aacggaaaag agccagggtg gctatacgcc cagctatcaa agctgggatg cctggggata
     6661 cggagcgggc aaccggaaaa atgacacgcc gcaggtcgat ccgaaaaagc cggatgcgcc
     6721 aggcacggtc aaaaactcca cgatgggtgc gccgagcgtc gatcaggtca acctgtccga
     6781 tctaaaagtt ccagcagatc agcgcggccc ggccaatggc ttctacgacg agtcggtggc
     6841 cggcaccgtg ccctggaagg aaaagtgggg ctcgggcaaa tacaacaaag acggcaccag
     6901 cgccgacctc tggagcgggt ctaccgttgc cggtgcgaga cggcaagcgg gtctgcgtgg
     6961 tggcgtttcc gtcgaaggtg actacggcaa agccgaagca tcgggcgacc tctacgccaa
     7021 tgcggaagcc ggcgtcggtg caaacgccaa agtcacgaac acgggtgtgg aagccggtgc
     7081 gcaggccagc gcccgcgccg gcatcggcgt gcgtggtgat gcaaacctga ccagcgccga
     7141 cggtcggctc gcgtccatgg acggcattgt gaccgatccg ctcaacgcag gcgtcggcgc
     7201 ccatgccgac ggattcgccg gcgtcaaagt gggggccggc gtcaaagctg gcatcggcga
     7261 aaagttcacc ggcattgaag gcaaaatcgg tggcttcgcg ggtgccgaag gcagcgccga
     7321 catccatgcc aacctgggtc cgctcaaggg caaggctggt gtgtcgggca tcgcgggcat
     7381 cggcttcgag gcttcgggcg gcatctcgct ggaagactgg aagcttcaca tcggcggcaa
     7441 gctcggtgcg gcactcggac ttggtggttc ggtttcgttc gacgccacgc ttgatctcaa
     7501 gcagaccgcg cagcttgcaa tgtgggccgg caagcagatc cacaacggtc tggatcgcga
     7561 tggcgacggc aaattgtcgc tcaatgacgc ggcaaaaggc gtcggcgagc tggcccaagg
     7621 tggcgccaac ctgctcgaca agggctggga tggtgcacag aagctgctcg acggcgacaa
     7681 ggacggcaag ttcacgttca acgacgtcaa gatccgcgcc ggtcaggcca aggactggct
     7741 cgcgagcaaa ggcagccagg cacttggttg ggcaggcgat caggtaaacg ccgctggcaa
     7801 ggccctgcac aacatggccg accgcgatgg cgacggcaag atcggcatcg gcgatctcgt
     7861 tgcaggcgca gcccaggcca aagagtttgc caaagacaag atcggccagg cgggccaggc
     7921 gctgggcaat gccaaagact gggcgctcga caaagcgaag caggccggca cttggctcaa
     7981 gaagtcggca gatcgcgacg gcgacggcaa gctcggcatc ggcgacgtgc tcaccggcat
     8041 cggcgaagtc aaaaacttcg ccggcgaaaa gatgcaccag ctcgggggcg cgcttggcgc
     8101 ggccaaggac tgggcggctg acaaggccgg tgatgctaag gactggttgc tcgacaaagc
     8161 cgccaacgcc ggcaaggcgc tgcacaatgc cgccgaccgt gatggcgatg gcaagctcgg
     8221 tttcagtgac atcgtcgctg ggatgggcca agccaaggac tgggcatcgg acaaagccag
     8281 cgccgcgggc aaagccattg gcaacgccgc tggagccgcc aaagactgga tcggtgacaa
     8341 agccagtgct gccggcaagg cgctgcacaa cgccgccgac cgcgatggcg atggcaagct
     8401 cggctggggc gatgtggttg ccggtgccgg ccaagccaag aactggctcg gcgacaaggc
     8461 ggcgtccgca ggaaaagcgc tcggcgctgc caaagactgg gcatctgaca aagcgagtgc
     8521 cgcaggcaag gcgctgcaca atgccgccga ccgcgatggc gacggcaagc tcggctgggg
     8581 tgacgtcgtc gcgggcgcag gccaggccaa agagtttgcc ggcaacgcag taaacaccgc
     8641 aggcaaagcc ctgggcgccg ccaaagactg ggcctctgac aaagcctccg ctgcctggga
     8701 cggagccaag agcgccggca agaccctcgt cggtggcctg tccagtgcgg ccagcaccgt
     8761 cggcggcacg ttcaagaagg cgaaagactt cttcggcggc tggtaacttc cgcgctccac
     8821 ctggctcgcc ggattccaca cggcgagcca cgtttcacca tcccgttacc gcaacatcag
     8881 gtctagctgt tctagctcgc gaatccggtc gcgcagcgag gccgctcgtt cgaagttcag
     8941 atcagcagcg gcttccttca tctgcgcttt cagcttggtc aaaagtgccg gaatgttggc
     9001 cagatcaagc ggcgcttcgt ccttggactg cgcctgtttc ttggccccaa acggcagacc
     9061 ggttttcttg tcggggcgac ctttgcgctc tttgtccttg tcgctgccat agcccggacg
     9121 cgcgtccccc gctgccaagt cttccgcggc gtgggttagc tcaccttgca cgatcgcgcg
     9181 cagatcactg accttgcgct tgaccgtttg cggggtgatt ccgtgcttct tgttgtgctc
     9241 gtcctgaatc ttgcgccgtc gggccgtttc atccaggcac acctgcatcg accgcgtaat
     9301 ccggtcggcg tacatgatga ctctgccttg ttcatttcgc gccgcgcgac cgcaggtctg
     9361 catcagcgac cgctccgcgc gcaaaaatcc ttctttgtcg gcgtccagaa tcaggaccag
     9421 cgacacttcc ggaagatcca ggccttcgcg caacaggttg atgccgacca gcacatcaaa
     9481 gaccccctgg cgcagatcac ggatgatttc tgaccgctcc agcgtgtcga tgtccgagtg
     9541 cagataccga actcgaatgc cgttttccgc gtaaaactcg gtcagctgct cggccatccg
     9601 cttggtcagc gtcgtcacca gcacgcggtc gccttgcgca atccgcagcc gaatttcgga
     9661 aatcgagtcg tcgacctgtg tcgacaccgg acgtacttcg atctggggat cgagcagacc
     9721 cgtcggtcgg atcacctgct ggaccacaac cccgccggtc tgctcaagtt catagtcacc
     9781 aggcgtcgcg gacacgaaca ccgcttgcgg cacccgatgg ttaaactcct cgaacttcag
     9841 cggtcggttg tcgagcgccg acggcagccg gaagccataa ctaaccaggg tttccttgcg
     9901 cgaacgatcc ccgcggtaca tcgcctgcac ctgcggcacg gtctggtgac tttcgtcgat
     9961 gatgcacaga aagtcgcgcg gaaagtagtc gatcagcgtg gctggcggtt cccccgcagc
    10021 gcgtcccgac aagtgccgcg aatagttttc aatccccgag cagtggccca tctgatccag
    10081 catctcgagg tcgaacagag ttcgctgttc aatccgctgc gcttcgacca gcttcatgtt
    10141 gtctttgaag ttggtgatct gctcgcgcag ctcttctttg atggcgcgca tcgcgctgac
    10201 cagcgcatcg gcaggcgtca cgtactgaga gccagggaaa atgctcactt cttttagctt
    10261 gcgcagcgtc ttgccacgca gcggatcgac ctcggcaatg ctgtcgatgt catcgccaaa
    10321 ccactcgatc cgaatcgcca cttcttcttc aaatggcggg aagacttcga ctgtgtcgcc
    10381 acgcacccgg aacgttccac ggtggaagtc gatgtcattg cgctcgtact gaagttccac
    10441 aagtcgccga agcaccgcgt cccgccgcac gctctgcccc acgtccaggc taagcaccat
    10501 gctgcgatag aactcgggat cgccaatgcc atagatgcac gagaccgaag ccaccacgat
    10561 cacgtcgcgc cgcgatacca ccgcgtgcat cgccgagtga cgcatccgat cgatctcttc
    10621 gtttatcagc gagtcctttt cgataaacgt atccgaggtc ggaatgtagg cttccggctg
    10681 gtagtagtcg taataggaaa cgaaatactc gacggcgttg ttcggaaaca gttccttgaa
    10741 ctcgccgtat agctgcgccg ccagcgtctt gttgtgggcg atgatcagcg tcggcttttg
    10801 gactttctcg atgacacaag ccaccgtgaa cgtcttgccc gaccccgtca gacccagcag
    10861 cacctggcgc ttgtcaccac gctggatgcc ttcgacaagc tgagtgatgg ccgtgggctg
    10921 gtcgcccttc ggctccatct gcgaaacgat ctgtagcgga gtgccctgcg acatcgtggt
    10981 ttagaaccgg tagcggacga tgatttcggc gttgaagttg tcagcgcgcc cctggatggt
    11041 cccaaaggtg gcgccgttgc gtgcatcaat ggacgtcgga atccccgcgc ccagcacgtt
    11101 gaagaagaac agcatcaggt cgatgcgctg cccggttagc ttctggaagt tgtaggtgaa
    11161 ctgaaggtcg accgtgaact gatctggaag ctggaagttt gcccaagtcg tcggatcgtt
    11221 gacccgcgtt cccgtcgatg tgccgcgcgg cgaacgatac agcgaaaacg acgtatcttc
    11281 tgggctctgg aatactttcc actgcgccgc gcccgttcgg tactgaagcc ggacgctcgt
    11341 gtttagacca aagtcgaacg agtaatccac cgcgcccttc agcgtgtggc gatagttgtc
    11401 agacatcggt ccgtagaaca gcggattcag ccgcggattc tgccggtacg ccgaaaagta
    11461 atcgctgacc gttccatcgg taaacgccag cgtataggaa gccaccaggc tccagttgcc
    11521 cggattgcct tgcgcccaca gatccagtcc gtgataggtg cgctgcgcat catcgggcgt
    11581 ttcggcctgg taaatcttcc gccgctcacc atttacgtag ccgacgatcc gcgtgcccgc
    11641 cgggtcccac acttggttta cttccgagtt ggcccacatg ttggcgtagc gacggaatgt
    11701 atagtcgacg cccacgataa gctcggtgat gatctcgcgg cgaatgccgg cagcgacttc
    11761 atccaagctc ggcggcaaga tgttgtctgc gaacagctgg ccgcccggac cgcccgaacg
    11821 attccgctcg acaaaagacc ggctggcgtt gtcccagttt ttctgaatcg caacctgctg
    11881 cggattgccg cggtcggcga tgtacgtatt tccgacgtcg ttgtggcgtc cgtagtgggc
    11941 cgaaatcagc gtcgagcggt cgtgcaaaag atcatagacc aaagacaggc gcggaccgta
    12001 accgaccagc gtccccagct tgtttccttg gtagtcacgc agcacgccga catcgaaacg
    12061 gaagcccgga accagcgtca ggtagcgctt gatggtgtag cgatcctggg caaaaaagcc
    12121 gatgttaaac gcttctgccg tcgtcgaaaa accacccgcc gccgacccat ctaccgcagg
    12181 cagtgagtcc ggatattccg tcacctggga acagcttgca tgcgtgtttg ggtccgccgg
    12241 atcgcaaggc gtacccggca cggtatcgac atagttgcga cccccagcga tgccgacgtt
    12301 gtggcggtag ctcatgtacg caaactgagc gccaaacttc acgttgtgca tgccgccgag
    12361 tcccatcgat gaatacagga acgtcgggtc gacctggacg cggtacttgg tttcttcgtt
    12421 ccagccaagc gagctgttta ggtacgtgac gccgttgaac tgatcggacg tgcgcagcac
    12481 atgcgacggc gtaatccgat ccggcaagcc atcgggcccc accgccatgg tgtcttccac
    12541 cgagcgcttg tcggtgaaac cggcctgcat ctggaacatc cacttgggcg acagcgccga
    12601 atcaatcagc agcaccaacc actcaccacc gcgaccgacg cgccgctcgg cttcttgtgc
    12661 cagggtgttc tgctgcgcgc tgttggtgat gtagttgcgg tcgatgttaa acgacaaaga
    12721 gacgcgcgtt cccgtcgtga cttgccaggt cagcttggcc cgtcccaaat aggtataggt
    12781 gtcccgaaag cggtcggtgt cttcagtaat cccgagccac ggaaacggtg gcacggtcgt
    12841 catcgctttg ttgtagttga actggaacga cgtaaaaaac cacagccggt ctttcacaat
    12901 cggcccgccc acgttggcgt tgaccgaata aaagtcccgg accggcggat cacggtcgtc
    12961 gtagatcagc gcttggtttt gctgcggcag ctgggcgcca tagcgcgtgc gcaggttgaa
    13021 cgccgtcggc tgaatcacca cgctggcatc gagcgtgaaa ttgttcccgc ccgatcgcgt
    13081 gaccatgttc tggaccagac cgagcgagtt gtattcggcg tccatcgcac cggtgaacag
    13141 gttgtactgt tccacggcgt caaagttcat tggcgagcta agcccgcccg tcacgacatc
    13201 ggaagtatcc agaccatcga cgagaaagcg cgtatagcgg gaaagaccac cacgaaccga
    13261 gggcgctgtt cccggcccga cacccgcggc aagctgcgca atcgactgga cctgccggaa
    13321 taccggcgtc cgcgacacct tttggttgtc gagcgtcgtc gtggaaaccg cgctttccgg
    13381 attcaggatc gggttgcgct tctcaatgat ttttttcgtt tcggtgatct gcccgacatg
    13441 cagcaccaca tcgaccgcgg cgagctggcc cgccaccacg acgatgccgc gctgctcaac
    13501 cggcgcaaat ccttcgatct tggcttcgag ttcataagtt cccggcggca agctctggaa
    13561 caggtagctg ccgtcatcgc cggtttcgat ggttcggctg ccacccagaa gctgctttga
    13621 tcgaacgacg atcggcacgc cggacagacc gtctttggtt ccatcgatcg taacgacacc
    13681 gcccagacgt ccggtgactt caccaatcgc ccacagtccc cgcggcacaa gcaacagcac
    13741 aacgagcagc agcgccgccc acagccctct tcctctcttc atcggcggga ctcctcgcta
    13801 cttggccgca gccaaggaca gacagtgaac gaacacgtcc ttccctggtg ccgtcggcag
    13861 gatcgcgccg gtgctcgcct gtagctcagc gattgtgcac agcggtcgag gtttgatcgt
    13921 ctggttttct ttggccgcac aggttgcgcg gcttagatcc acccagcgca cgcggcagac
    13981 cggcgaatag tttggctgac ccggtgaaac cgcaaacatc gccagtgcat cggctttgcg
    14041 cgtcttggtc atgtccatcg catcggtcag cgtgacaatc gacttgctgc gtggcagcgg
    14101 atcgctcgaa tcaaagctcg atgcagacgg tggatcaaac tcgtccttcg atacgtagag
    14161 ttcgtttatg accgggcaga caggtggcgg aacaagacat gttccgctct tggctgggtc
    14221 ggttgccgtg cagacttcgc catctgcgac gctgcagccc gtgaccattc ccatcgcgtc
    14281 gaccttgcag ctcttgcccg agtcaaacag ggccgggcac ttgcgcgctt ccgtgatgag
    14341 cggcagatcg ccaccatcga catagcccga cagaaggcca cggaaccaag cctgggaggg
    14401 gaactgaaac ccagccgccg agtcgctcaa gttctgggga tagacggctt tgctgcccgt
    14461 cacaaacgga cacgcttgga tcgggtctaa gctgcggccc accggaacgc cgacgttgac
    14521 catcggccag tctttgaaac ggaccgcgcc agatcgaatc tgctcacggc ttgggaacgc
    14581 aaagtcaaag tcctttggcc ctgtgtccgg aaaggtcttg gttgcgcgat cccagccggc
    14641 ccgttccaaa agggacagct cgcgcttcac atcgttgcag cccatgcggt ctcgcacggt
    14701 cgtttcaagc tgaacctgca cgaccaaatg gaacggctta tacgatgctt gcgccgtcgg
    14761 ctcgcccgcc gatccgacct gcaccggaaa cagtccttgc gacaggatcg gatactgcac
    14821 gcgccggtcg tagttattta cgcggatatc aaactcaccg gggtctttgc gaccttccgg
    14881 cgcattgcaa cccgatagcg aatacaccga tgcaggcaga gcccgcgtgg tgtcaaaggc
    14941 acccgccaga tcaagctgcg cggcgaacag agcccccagg taatagaagc gcggcgagtc
    15001 actcagcgac gcgccatccc agccgcgccg cggcaaaagg cagccgttta ccggatcttc
    15061 gcgagaggcc ggctgaaact tgtactgctt gtcgaatgcc gatggatcga tcgttccggt
    15121 atacgggtcc ggatcgatca cgccactgca agcagccgac aaggaaagca agcagacggg
    15181 aagaagcgac aaaccgacag agcggacgag cgtgcttttc tgcgacatcg cgccgccgtg
    15241 taacacgttc gacccaagca agacaagcgc cgtcgaaaac atcgacgaat cgacaggcgc
    15301 aacggcggtt ttgtatcggg aaaaggcaaa cgcccagcta cgcggtggcg tctttcacac
    15361 ccagtcgcaa aatttccgcc aggtcggacg gaaccggccc gcgacgcaag gggttcacat
    15421 tctgaccgct tgccccaacc agaccgcgca ccagcgcagt tgccagcgga ttggacaaaa
    15481 ggatcagagt ctgccgcaca aggcgtgaaa ggtcgccccg ccgcaagctc cagcgcatca
    15541 ttcccaagtg cacacgcgca tgcggactgt tccacgcttg tcccagcaca tgcgcccgct
    15601 ccaaatgccg ccatgcctcg tcgaactgct ggcgtgtttc ggcttggcgt gccttcgcca
    15661 gttcctgttc aaaggcttgc aggacgcggt cgggaatctt gcacggaatg cgggctttgc
    15721 tcatggggga cgctctccgc gcccagacta gcatgacctg gacgtttccc cggacttggc
    15781 cagcccgctt ctgccttgcc agactacttg gcgctggagc ccagcgtcag cgctggcaaa
    15841 tcaggggcag acttggtgat cacatgcggc ttgcgctgcc cgacccactg gtccatctgc
    15901 gtccactgct ccagcagcca ggaaatgatt tcctctcggt cctttggcac ctcggcaaac
    15961 ggaattcgtc ggaagcggac ttcaatgcgc tgatgcagcg cccgaccatc gacaagatcg
    16021 ccgaacgtcg ctgcaacttc atacccaacg tgttccacaa tcaggacatc cgctgacgga
    16081 ttccgacaga gcagctcgac cgcgccacca agctgcggcg gcatgacgtg ggatagctgg
    16141 gaaacgcgcg cgtacagcgc cggattgtcg cgctctagct tggccatgac ctgaagccgc
    16201 ttggcatcgg tgtagcgggt tccttccgga tacagaatga ccgcatcgcg cggcgacaga
    16261 tgttcaagct gcgcacagac cgactcgatt tctcgaccgc tgttcttgga accacgccga
    16321 acaaaacagg aaccgagccg accgccgatc acatcgaaca ccgggtcatt gataagctcg
    16381 cgcttgacga tgtagcgcag ccggaaacca tagcgagagc ccagatacgt cggcgcaagc
    16441 ggcgtatcgc ccaagctggc gtgacgcaga agcacaagca ggggccccgg tttttccagc
    16501 tgctccaggc cgtcaaacga caggcgcaga tcgaagctcc aaaccccaag ctggtagaga
    16561 acccggcccc aggcgttgga aagactctcg gcggcatggg ccaagcggac ctgtgaaccc
    16621 ggcaagaaac cgagcagaaa gacccactgg agaaacagca ggaacaaaaa cagcatctcg
    16681 ccgccaaagt agcccgccaa aaagaagatg aagcgaagac gagcaaacgg gtgacgctca
    16741 aacgcagccg tgacaagacc tgccagggca agcagaggca aaaggacaac aatcagcgtg
    16801 ccgaccaggt agaaaagcgg aatcgtgacc aaacgtcgac gccaggtcac acgccacgga
    16861 tcgccaaacg atgaaatacg agcgaggaag tcaccactac tgtcctgcat gacgcgaaga
    16921 tatcggaaag cattgccgac ggtgaagtga cgacacaaac tccgcagaaa aatgacgacg
    16981 ctgtgacaat tccatgacaa gatcccccaa tccggtctgg ccgcacggcc acttgtccgc
    17041 aaaaagtccg caggcatgct ggccgcactg ccgcccccta tcttgaaagc caagcagcgt
    17101 gtggccgaac tttggccgcg tttgtgtttt gcaattcgat tgggtccgcg ccgccggaat
    17161 tccgaagaac aacgcactac cgattggccg gaagtcggaa acctcttggg actgtggatt
    17221 ggggctgtcc gcgcctgcga acccaagcgg aggtggcata aacgtcgcgg ccccactgcg
    17281 accattcaac gaacgcagta gaaaaatgtg tggccgcaca acccgatggg aaaagaaaac
    17341 cggatggccc gcggtccgca gttcccaatc gggttcacgt taccagctca tgctggcttt
    17401 ggcgggggca gcagcgggag cagcttggat gttctgctcc aggtcggaaa agccgttttt
    17461 gcgaacgcgg actttgtact gaccgcgatc cacagacttg aaacgatagt tgccttgctc
    17521 ggtcgttcgg gtggactgca ccacgtttcc ggaatcgtcg acaagctcga cgagcgtgtt
    17581 ggcagaacca ctgccaccac gaagggcaat ctggccttcg acttcggcaa acttggcgcg
    17641 ctgggcggct tcgctgccgt ggaaaaagat ggtgctatcg gtctgacgga ctttgccttg
    17701 ggcatcgcgg aattgaactt tggcgacgta acgaccgacc ggcagcgcct tgccatcgct
    17761 gccttgtccg ctccaactga acgacagacc gccaagttcc cccttcttgc tgctcacaaa
    17821 gacgcggtct ttgggttcgc ttgtctttgt gtctttgccc gcgtccttgc cgccgtcttt
    17881 gatggtgtag acctccattt cgacggaccc tgactgctcg gactgcgggg cgatgttgcg
    17941 cagcgataca acggacgagc tgctcttttc atcgaactgc gcccccagct cggtcataaa
    18001 cacatcgcct gcgtacacgg tgtagcgacg gaccgagcgc tgtcctttgt ccgtcaccgc
    18061 gacaaaatca atcatgtgct tgcctgccga aagctgggcg gtgtcgagcg tgtatgccag
    18121 cttagcaccg cactctgtat gcaccgatcg atcgtcgacc aggacttcga tctgcttgat
    18181 tccggccccc tggttgtccg ccgcagagac cgcaagcgtc atcttgggtt cgacgtgctg
    18241 accatccgta agtcctgtca ctttcagact ggccacatcc acagtgacca gcccgagtac
    18301 ggcccacatg gtctcggctt tttccgcccc accctgcccg ctgcgatacg ttcgccaggc
    18361 gccgctctgg tcctgggtgc tgaccagaaa acggatgcca cgcgccacct gggtatctgc
    18421 atcggaatat ccggccagct tgagcgcgta cacggtctgt ccggttgcaa acgcatcact
    18481 cttctgcgga tcaagtcccc agccgccgtc gccattttgc cgatcgagca gcttcttgat
    18541 caggttgtgc gcgctggctt cattgcgcgt cacaccggat gccaaaagtc ccagcagcga
    18601 aaagttgatg tcctgaacgt agacctgttt gccactgcca aagctggcgg agtgttttgc
    18661 caggtatgct tcggcacgcc gcatcggcga cagccatttc tgttctgccg tgcgggcata
    18721 ggcttgtcgc cacgtctggg ccgcttggaa cgtcgtttct agcgttccgc ccgtgaccgg
    18781 caagcggcga tcatcgtcat tgatggcgcc atctttgccc tgaaccgaca gaagctcctg
    18841 cgcataccga agcagctcgg cggtttcgtt gttcccgacc cactcgtcat aacgcgccca
    18901 ggcgctcccc tcgaacgcga tgccggtggt atggccgccc gctgtcaccc ccattttcag
    18961 ggctttgacc atctctgcga gatcctggcc agcgatggtg tactggtgtt tttttcccac
    19021 cgagagtgct tccatcgtca ccgcttggac gtggcagcca aaacagcggt gctgctgcgt
    19081 ccaggcctgg ctcgctttcg ataggaaggc cagtccccgg ctggcgctgg ctcgaatctg
    19141 cggatcgttc ttggcggtat ttttgaccga ttccgtcaaa caagcagcgg cctttggtgc
    19201 aagcggggcg ctcgcgaaag ccgaagcaga ggggagcaca gcgatcgcga gcagcgacga
    19261 agaaaacaga cgagtgagcg agttcatgaa agcctccggt atttctgccg ggctcaaacg
    19321 tcgtccgttt gcaaaggatc tggacccatg cggcgggcgc gaattttttc tgcgcaccga
    19381 cgcagccgca gctatcggcg atcggtaagc ctgcgggtca gcgccgattg aatgatcaag
    19441 atcacgaaga tcagcaccgc atacgcagcc gcaaagccgt agcgatgtcc gcgggtgaac
    19501 gcccagcgat aggcctggga aaccaggatg tcggtggcgc cgtccggttc gccgccggac
    19561 accaggaaca ccacgttgaa catattaaac gtccacaccg cgcccagaat caccgaaggc
    19621 agcatcgccg gcagcaaaag cggaaggaca acgtgacgca gccgctgcca caggttggca
    19681 ccgtcaagca tcgccgcttc ttccagttcg gacggaatct gcgaaagatg tcccagcgca
    19741 accaccatca tgaacgggaa accgagccag acgttggttg cgatgttggc cgcgagcgcg
    19801 gtcgaaaagt gactaaacca agacacaggc gcaatgccca gcagctggag aagaccgttg
    19861 atggcgccaa actgccggtt gaacatgccc ttccagataa gcccagtgat gtaattgggc
    19921 accgcccacg ggacgatcag cagcatgcgg tagacaccgc gcagcgcaag ccacggactg
    19981 cgcaaaagca gcgccagacc cccgccaatc accagatgca agcccacgtt ggccaacgtc
    20041 cagcagatcg tcaccagcag cgtgaagtag aagctcatcg gctccaggca gccaccgccg
    20101 cgacacagca aaatgtcggc aaagttttca gccccgacaa accgccaact tccgtctaga
    20161 tcgcgatgga acagcgacat ccccgcgccc actgcaaacg gaatcaggga caaaagcagc
    20221 atcgcaaaca gcgccggccc gatgtagccg taggcgtccc gctctcgcca tgcttcgcca
    20281 aagacatcgc gccgtcgcag ccgcaggtac tgccaaacac acagcccaag caccaccaca
    20341 gacagcgcca gcaaatatgg tctgtcatcg gcagtcgcgg ggggcgggcg caggtattga
    20401 tcaagcagcc gttgggccgc gcgcagcgca tcttgcggtt ttccaccgcg cagcacctga
    20461 cggagcgcct gctcacccgg ttcccagaac gcgcgcatcg ctggatgggt ggggcttggc
    20521 acgatgtgct gacgctgggc gaaaaaagcg ctgagcaccg gatcgttttg cacttcgggc
    20581 cggtcccacg tcgtcttttc cgatacagac tgccggccca ccgtggcgcg aatctgggcc
    20641 gactcggcgc tggccaagta gcgcaggaaa tccgccgcct cgcctttgtg ttcacttttg
    20701 gccgacacaa accccgcttc aatcgaggtc agcggcgcag caatctggcc cgtttcggac
    20761 accacgggca tgggcgcaac gccatagggg acatccttgg caatctcggc gataaaccac
    20821 ggaccattga tggtcatcgc ggcccggccc tcgttaaaaa actgggtggt caggaccgcc
    20881 gacatctctt cgggcaagtc gccgttttgg accagctgcg aaacaaactg gagcgcggca
    20941 agctgttctt tggtgtcgag cgtgggtacc gggctgcccg gcggaatgat cgcgccgcca
    21001 aagccgtgga gcagcgccgc atgcttaaag aagttgccgg cttcatagac caggccatag
    21061 cgacggctgc cggcgggact gtgggccttt tgctcggctc cgatttgcag gatgtccgcc
    21121 gtggtccgcg gcggcgtcgc caccaggtct ttgcgataaa acagcgccag cgttttccac
    21181 gacagcggca gacccaaaag cgcgtcgccg tccttgagcg gtggaatggt ttccggccac
    21241 agcgtcggca ggacatcagc cagcggcagg tgcgaattga gcggttcaat gaggccgagc
    21301 ttcgaccact gcccgacaat gtcgtgcgcc tggataaaca ggtccggacc gtgcccgcgc
    21361 gggatcgccg cttggagctt gtcggcaaag ctaccgtagg gaacgctgac cgcttcgacc
    21421 tgcggcccct gcggatgctg cttttggtat cgctcgacga cctcggtcag cgccttttct
    21481 tcgccgcccg cgtaggcatg ccaaacgacc agcttggtgg caagaagcaa aggcagcgcg
    21541 tgcatcaggc cgccgcctcg ctcttttcat acggggaaag ccggttgccg tcccctttgt
    21601 catcgcgacg ctgaaacaga tgacagtttt cgggcgatac caccagatga acgtcggtgc
    21661 ctggcggcgg cgacttgtgc gcaatcgatc gaaccgacag cactttgtcc ccgcagcgga
    21721 cgtgcagcaa gtcttcgtgg ccgtgatgtt ccacgacatc gatgcggccc cgcggcagct
    21781 cggtgccatc ggctttgtgc aagtgcaggt cttcggcgcg cacgcccagc gtcaccggct
    21841 gatcggcaaa gcccggaacg tggacacgaa acggaatgcc gtccccggaa aaccagccgg
    21901 cttcgatcgt gccgtcaatc aggttcattt ccggcgtgcc aaaaaatcgc gcgacgaacg
    21961 tgttggccgg gttgtcataa agctgcgcgg gagtgcccat ctgttcgatg cggccttggc
    22021 gcaacaccgc aattcgatcg gccagcgtca tcgcttcgac ctgatcgtgg gtcacgtaga
    22081 tcatcgtcgc tttgagccgc cggtgcagac gcagaagctc taggcgcatc tgctgccgca
    22141 gggcagcatc cagattcgac aacggttcgt cgaacagaaa cgctttggga cgccgcacga
    22201 cggcacgacc catggccacg cgctgccttt gcccacccga cagctcgcgc ggatagcgcg
    22261 acaaaagatg cgaaatctcc agcatctgcg ccgcctcttc caccagcttt ttgatctcgg
    22321 gagcggcagt gcggcgcatt ttcagaccga acgacagatt ttccgcgacg gtcatgtgcg
    22381 ggtacagcgc gtagctttga aacaccatcg cgatgtcccg cgcctgcggc ggcacgtcat
    22441 ttacaacgtg gtcgccaatc gccagctcac cactcgacgg ttcttcgagc ccggcgatgc
    22501 agcgcagaag cgtcgtcttg ccacatcccg acggaccgac gagcaccaga aactcgccat
    22561 cggcaatttc catcgagatg tcatgcaaaa tccgatgcgg tccgcccttg ccaagggccg
    22621 acggcttgtc tttgcacaga cgacgaatgc tgacgtttgc catgcgaaac gagggccaaa
    22681 gtgtaccagg ctttgcagcg agttggccct acatctcagg caaatttggc aaaagctact
    22741 gaggtttgcc gttcacaggc aggatcggcg cccaaatcag cttggccagc agccggtcgt
    22801 ccaccatcat gccgcggtcg ctgaaaaaca gggagttgtc ttccccgtct tcctgccact
    22861 gacccacgaa gatgatgccc ttgccctctt cgttatagcc cagttcgaca atcgcgttct
    22921 tgagccactt gccgccgccg tccatgttga tggtttcggg cagcacgtaa aaaccttcgg
    22981 agggaagcgc cttgagcgtg cgcgcccagg ccgcatgcgt tgccttcagc ggcgtggtcg
    23041 ggtcctgcca aaaccagcgg ttgcggcggt tttggcccgg acggaccacg aacttgacgc
    23101 cgccagtctt cggctggccg atgaaaacca gaacgccggc gggaatcgcc gattcattgc
    23161 ccggcatcgg ctgcgtggtg cggtataggc caggatcagg cagatcgaag tcaaacatgg
    23221 gcggtgttct ccaaggtttt tcaggggaaa gcctgcgctt tccggtttgc tcatcactgt
    23281 aacatcgcgt gtcggcacgg aaaaaccgca tccgcagaac cgacggcacg ggtgctgtac
    23341 gaaaaaccaa gctggggatg acttgctgtc acggcaaggg ccgatttgcg cccacatcac
    23401 gggctaacgc ttgctgcccg tcatagccat ctgataggct gccgctcacg atggtcgggc
    23461 gtaccctcgc gcactatcga ctgcttgagc ttgtcgggca tgggggaatg gcgtcagtct
    23521 tccgtgccca cgacgagcag ctcgatcgcg aggtcgctat caaggtgatg catccacacc
    23581 tggcgaccga ggcgcaggcc aggcagcgat tttctcggga agcacgcgcg gtggcgcggc
    23641 tgcggcacga aaacatcctc gaaatctatg ccgcaagtga tcccgaatcg cccgaaagct
    23701 acctggtcac cgagttcgtc agtgggccga cgctgcgcaa gttctttgag ctacatggcc
    23761 cgcttcccgc cgagattgtc ggtctgcttg gctgggttct cgccgacgcg ctcgcagctg
    23821 cacacgaatt tggcatcgtc caccgcgacg tgaagcccga aaatgtcatg attcgcaccg
    23881 acggtcggat cgtgctgtgt gattttggca tcgcccggct gctcgacaac gactcggtga
    23941 caaatacggg ccagcttctg ggttcgcccg cgtatatcgc ccctgaacac atcagcggac
    24001 agccgcagga cggtcgatcc gatctgtttg cgcttggggt gctgctgtac gaagcgctgt
    24061 cggcagagct gcccttttca ggccgcaatc cccacgaaac gctgacgctg attgcgcgcg
    24121 gtgaacacaa gccaattggc gagcgcgtgc cgggtctgcc tcggccactt gaacagattg
    24181 tcgaaaagct gctgagctgt tcgcccgacg accgctatcc gaccgctcgc aacctgggct
    24241 tggcgctctt ggacttcctg cgggacgtcg gcctggcgac accacgagag cagcttcgca
    24301 gttactttct ggaccccgag ggattttggc agagcttttt gcctcagctc gtcgagacgc
    24361 tgataaaaaa tggcaaagcc cgcgccgaag aaggtcgcac cgctgccgcc ctttcagatt
    24421 ggggtcgggt gctgcttctg tcgcctggtc acgccgaagc cgaggagctg atcgcccgtg
    24481 cctcgcggct caagcagcgc cgccggatcg tccgcaccat ctggatcagc atcggcgcgg
    24541 ctgcggcatg cgtcggtctg gcagtgttgg ggcaaagcgt actttcccgg cccaaggtgg
    24601 cgccgccgct gcacatcgtc gccgcgcagc aaaccgctgc ctcggcagtg ccgatttccg
    24661 ccgacaccga caaagcgccg gtcatatccg tcgatgatcc ttcgccggtc ccagcggaaa
    24721 ccgacctggg ccaagacgca tcgacaagta agcccaagct ggttcaccgt cccttgccgg
    24781 tcaagtcggc agcccccacg catgtggtcc ggctagagcc gtggccgaag tctgtgacgg
    24841 tgacgcacaa cggcaagcgg cttggcgcct atggaaccga cgtccgcacg gtccagcttg
    24901 gccccggccc gaacgaattt ctgttcgaaa gccaagcctg ctacagcgaa aaaatcaccc
    24961 tgcccgctgg cagcaccccc gaagaagtcc gcgttcgcct gcgctggaaa ccggcccttt
    25021 tgcaagtcag ggcccacagt ggaaggctcc agccgagcga tcccgtggac attttggttg
    25081 acggtcacat cgtcggacgc ggcggacagg tgatggcgat tccggtctct gccgatgaag
    25141 gccatcaagc cgtcagtgtt caagtcggcg cgcccggtca caaaacccat accgaaaacg
    25201 tacaagtacg ggcgaaccaa cttttgcagc tggatgttga tctgccgatg cgctagctcg
    25261 cgataagatg cggattgatt cgcggcatgc gcacggcatg tcgaccaaac gaggggccag
    25321 atgaaacgac tgtggggtct gctggtgtgt atgagcctgc ttttcgcagc gggctgttcg
    25381 gggctgtaca tgaacattcc gatgacgcga ccagccagcg tcgatatttc cggaattcgc
    25441 aagatcgcag tgctgggctt gccgcaaaac ccggacacgc ccccttcatt tggcagcgag
    25501 ctgacgggac ggctggcggc aaatctcacc agcaatggct acttcaccgt cgtggagcgc
    25561 ccggacctaa tgcagcttct ggcgcagctg caaatgccgc ccgaagcact caatgatccg
    25621 gtgggtctgt cgcggatcgg ccaggggctt ggcgtcgatg cgatcatcat cggaagtgtc
    25681 gactcgctgc gggtgcaaga cacaaccgga gtggatctgt tggatgtacg catcccggtg
    25741 atcgccccgg tcgtggcggt cgcgccgccg atgggtggca ccgttgtgct gccgcctcca
    25801 ccgacgccgc cgccggtcgt ggtcgcgccg cccccgcggc atcggcaccg cacgacggtg
    25861 acgccgccac cgccgccgcc ccctccagcc gccgtcgagc ccggttatgt gatagagcgt
    25921 cgcccgttcc cgtttgtggt ccgcacggcc aatctggtga tgaagcttca gcttgtgcgg
    25981 gtcaaagatt cgcggctcat tcacatgctg ccggtaagct tcaccgcgag tggcaagttc
    26041 taccctggac cgcagcccga tccagcgccg agctatcaca gcggaccggc cctttcgaca
    26101 agtccctacg tgatgcgttc gctgcccgcg cctgaagcga tggtcagcct gttcgcttcc
    26161 gagatgagcg gcaccttcgc aagccacatc gtgcccacgc gctacaccaa gcggatcgag
    26221 ttcgcgagcg acaaaaaccc cgacaatcag cgcggcctgg agttctgccg gtcgaatgca
    26281 tggaacttgg cctatcaagc gtttaccgag ggactggcca aagatccgaa caacgcggcc
    26341 ttgcactata acctcggaat tttgcgtgaa tccgacgggg attacgccac cgcaaacgcc
    26401 gaatatcagc gcgcgctgag catcgatccc agcaagaagc tgtttcagca gacctatcgc
    26461 cggctgcaag actccatggc cgaacaagaa gtcgtccgcc gccaaatgga agcccagccg
    26521 taatccgcac gagcccgatc ccgcgtcaca gctcggttgg aatctgcacc acaacgggcg
    26581 acgggctctt ggggtcccag cggcacagag acagggcatg gccacgccgc acctcgctcg
    26641 gctcggttga aaaccacagg tggcccagca cgtcgccgcc cagcagcttc acacatccga
    26701 cgggccgcac aacttcggtc aacgtggccg tttccggatc gatgcgaaac aggcggtgct
    26761 cgtagagatt ggtcatccac agcgcccccg ctgccgcggc cagcccaccc atcgaaaagg
    26821 tcgttggcag tggcagcacc ttgtgcgagc gcagaagcaa catgtttggc cccccctgga
    26881 cactcaccag caagtcgcca tctgccgtcg ccccgcgcag ctccgccggc tcacgaagaa
    26941 caagctgcac cgtccctgac tgggcatcca gcagaaacaa ccgtccgccg cgtgtcatca
    27001 ctgcgcgctg tcccgacagc ggctcgactg atgccggcag atccggccct tggaacacct
    27061 gacgaaaccg cggtgggtcc acaaatggca gccgcgcttc gacccggacg gtctgcgttg
    27121 cttcgtcttt ccacaggcac cagaccagcg gatcgccctc ccgctgccgc gcatcagacg
    27181 cgggcagaga ctccggcggg ctgcacagcg ccaccgtcac gatgtaacga ggcacaagac
    27241 ccgctggcgg cgacagcgtg tcgtttgact tgcccacaaa acgtgacgga gcaccttctt
    27301 tccacagcac ccgttcagcc ggcgtgtatt cgtctgcaac gaagaactcg ccgccgctct
    27361 tgcgcaggcc caaagaccgc gacttcgaaa acagtacaaa ctctgcagtt ccatcggcgc
    27421 gcggctgcat ccacgacacg tagaccgacg cgcagccccc gagcgaaagc ccagtcagca
    27481 gcagcatcag catccgaacg gcaaccccaa cgcgcgacat cgacatggcg tcacagtacc
    27541 tacgccggaa gcggcgcgga cccaactttt cgcaaatcgt tttggtggga aaagtgagcc
    27601 ggcccagacg gtgtcttgcc ttacgataga cgcgagggat tccgatgatc agtctgctgg
    27661 cactgtcggc caccatcgct tcgggcagtc acttcacctg cgccatcggg agcggtggcg
    27721 ccgtgttttg ctttggggca aacgaatctg ggcagcttgg tgacggcagc cgcgagtcgc
    27781 gcggaaaacc ggttcgcgtc gttggactca ctggcgccaa agacatcgcc gcgggcgaca
    27841 cccacgcttg cgtcgtcgat gccgaaggtc acgttctttg ctgggggaac aatggtccgg
    27901 ggtcgtgcgg cgttccgctc gacatccatg tcacgacgcc ccagcgggta gaaggactgc
    27961 cgaacgcgag ccgcgtgttc gccggccaca gcacatcttg cgcggtcagt acggacggac
    28021 ggctgttttg ctggggacga aatctgtcaa accagctcgg agtgccacgg ggtccacacg
    28081 aagccggacg gccaagtccc gtcgaaatca aagagctgaa agacgtgcag tccgtcgcca
    28141 tcggcgattc gcacacctgc gcactgacaa agcgcggcga ggtctactgc tttggaagca
    28201 acgaacatgg acgcagcggc ggccaaggca gcgtggttcc cccgacacga ataaacatcg
    28261 gcccggcggt ggccattgcc gcgaccagca atgcatcgtt tgcgaccctt aaggacggca
    28321 cgctgtattt ctggggaagc tggcagcgcg accgcgactt tgtgatggca cctgtgccga
    28381 ccccgaccgg cgaccgcaac gtcacgctgc tttttaccaa tcgccacagc gcgtatgtgc
    28441 gcagcggatc aaatgactgg cgctttccca aggccggcgt ggcggccccg atgccgccga
    28501 caggcctttc gtgggccgtc gaggtttcgg caggtgaaac gcacgcctgc ggacgggacc
    28561 gcgacggagc cctgcgctgc tttggcacaa accgcgaagg catcggcacg ggcgttccct
    28621 ggaacggcaa agaacccgtc gtcgtgccgc tcgatgcccc cgaatcggac ccacttggca
    28681 cgaccgcgca gccaccgcca ccgcccgcag cctgtgaagc gagcgccgcg tcaccatcat
    28741 ggcaacaggc caaaccgacg ccgcccacct gcggcaatgg caagcgagat gtcatcggca
    28801 tggccggtgg gcagtgtccg ccctgcatcc cggatcgcga atgtcactgc gccccgcgcg
    28861 tcgagctgcg ggaagcgtgc gatggcaaag acctcggtgg cgcgtcgtgt tcttcgcttg
    28921 gctacggcag cggcaagctc gcctgcacgg cgcagtgtac ggtcgacaca agtggatgcg
    28981 tgccggcaag tcacctgccc agcggagcgc gcttggcctt tgttgagctt ccgatagaca
    29041 agacgccgac cgccggcatg gccatcgcgc cgaacgacaa cgtgctgggg gcggtgtggg
    29101 gagccacggg tcagtgcgga caggcggtgt ttggtcagtt ttcggcggga ctcaagctgc
    29161 tgtcgacgtc agcgccgttt ggacgcccgc acgcaagccg gatgcagctt gtcgcgactc
    29221 cgagcggctg gctggcggcg gtgggcgacc gactgaacac ctgggtccat ctaattgatg
    29281 caagcggaca gccggggccc gagcaggtcg cgttccgggg tcggcccgtg tttttgcaga
    29341 gccaagggcc aaacggtccg tacctgctcg gactgtcgac ccgcaatcat ccgtacatgg
    29401 gcggtctcga cacggtgctg ctcagcggaa gcggcgaggt gctggccaaa tcgacggtgt
    29461 ttggccacca gtcgtcggca tccgagacga tggattcgta tccaccgccc gccgatcaag
    29521 tcgccatcac gacgctggat gacgggtttg cggttgcacg cgcccaactg gtgccaggcg
    29581 tcatggatct gggcggcatc gtcgtggcgc ggctggccaa agacggccag gtccaggcca
    29641 agtcactcat cgcgataggt ggtacgtcgc cgtttttcgg ttcgcacaag ctgctgggct
    29701 ggctgtccaa tctgtcgggc ccaggggaaa cccagcgcta cggcgtgcag accgtcagca
    29761 tcgatgcaaa cggcgcggcc caaggcgagc cgcagcttgt gacgaagctc gatccatcgg
    29821 aatggatcat cacgggggcg ctcgcgggcc caagtcctgt gctgcttgtg ccgcaggtgg
    29881 atgcgtttca gtcgacgttt cagtcgaaca cggcccgccg aatcgatctg ctgacgctgc
    29941 ccaagcagcg gctgacggtg atagacggcg gcggcatggg ggcggccaag ctcttgcgct
    30001 ggaacgatgc gatggtggcc ggctttgtct ggcagcgcga cggacgcgcg cgaatcggtc
    30061 tgatgacagc acccgcagcg aaataggggt cgaagccgaa gccacgaggc ttggccccct
    30121 cgacccagaa gaacaggccg agctggccgc actcacgaag ctgctcgccg gtcggttttc
    30181 accgcgaaag ccccacccgc gcgcgacgcg accgaccaca ggctgccgac gtgagcaaat
    30241 c
//
LOCUS       DAORGN010000037        30032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_572_length_30032_cov_6.088901, whole genome shotgun sequence.
ACCESSION   DAORGN010000037 DAORGN010000000
VERSION     DAORGN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 30032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30032
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_572_length_30032_cov_6.088901"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1621)
                     /locus_tag="PKL17_06390"
     CDS             complement(128..1621)
                     /locus_tag="PKL17_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44390.1"
                     /translation="MRLALRLTMLIIFTFVLALSAITWEQKGRFEWRYEEMRTEQLQA
                     LLTALDEGANLAWHDGGEMSLRRYLDRVAARQDYSSVHLVRLDALSKDDLFAAFPTEL
                     RKQAQAGEQVIFIDHQKSPPVLVAFDRLSLGQEAGQRFGVTLRRAMQGESEFVSENLR
                     TFIKNTGLTVLVGVIVAVLLGARLISAPLNRIVAKARLVGKGDFGVRFLIGNESSAEI
                     TLLSTELDAMVERLGELHRRVESEAAARVAVVEKMRHNDRLATVGTLAAGIAHELGTP
                     LHIVAGRANRIATAQAASDDVRAEAESIRSQCERMRIIVEQLLTFARKPSGQPSTVSL
                     QDVARRVTAWLTPVARKKNCELSLVPPSADIRCIAHVGLIEQALTNITVNALQAVGED
                     GIVRVAVREEVRSDKSPGGKPQKWAVLEVSDNGSGIAKEIRANIFDPFFTTKAVSEGT
                     GLGLSIAHEIVQEHGGFIEVTSRTAGESASGGEHGTTMRLYFQAEAA"
     gene            complement(1808..3208)
                     /locus_tag="PKL17_06395"
     CDS             complement(1808..3208)
                     /locus_tag="PKL17_06395"
                     /inference="COORDINATES: protein motif:HMM:NF025399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4246 family protein"
                     /protein_id="HNK44391.1"
                     /translation="MSSAQSAPHFAQSSDFAFVEELLTYGEGHPPQPGDIVEFEPGDL
                     ETTSGEPFPSDSALVVASGDETLTLKDQQSGQEMEVSSDGWFRIVESPQIQNIWEQHL
                     RRPCVDGIAYGDGLVDANLVGRLRANVKRLIDEEPVDYHPGSGTKVRDLVHPSLYPYI
                     EGKSVVVGSVPKAESPPYDRFGRPFESSRYQWLPTPFAIGSDGKIQIPEYINNLDRTK
                     YPALYDDLAALFATALPLVESVTGYVDSTKFWQEECDEIEHEGELPESGGDEAQTVKP
                     TSLRGRELQVIPKIVEYRLGQGETHEGVWHVEGMSHEHIVATCVYILDRDAAIQGGAL
                     RFKRAYTVEEAGRLFWNIDQCRPRPIEQLVDEGTIPLGSLETPEGRLLVFPNSHIHKL
                     SELSLVSGSSEGRRRVIVFWVVDPNVRLVSTREVAPQQTVIPKDEALAVRLELMEERK
                     RHKQSLNVRAVSLCEH"
     gene            3371..4807
                     /locus_tag="PKL17_06400"
     CDS             3371..4807
                     /locus_tag="PKL17_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK44392.1"
                     /translation="MNSSELALLHASLAELSDLVAFSSVLYRKMFERQPSYRALFAAD
                     LEPQLTRMAAAIRFGILHIADDTVLLPLLTSFAARHVEAGITNQMFEVVGLALQDALK
                     EQLGERFSDPLPRLWQSAYQKLSWALQERMWHAPQDGLPQMERAIREKLLLSSYDDLP
                     AVAPLHPVHDDCTVQFVGVGKVTVRRGDTLLSVARTHDIPMLGECGGHGRCTTCRVFV
                     EEGMEWLGPRTVAEEAIAQRRHLPPRVRLACQTNVLGPCRVRRLLHDERDIAAVLRRR
                     SGISGQQREIAILFADIRDFTPFVEQNLAHDVTHMLNRYFTAIVDAMEPHGAYVDKYI
                     GDGIMVLFGLDSQRHVHPCLDAVQSALAIQRAVSEQNPYFEKYFGHRFVCGVGVHFGP
                     AVIGDIGSWRKSQLTALGDTVNVAARIESATKQLGHAILVSEPVVRALHKLPSDLPAA
                     KSFVIGESVEAKLKGKSEPQNVYPVRPA"
     gene            complement(4820..6847)
                     /locus_tag="PKL17_06405"
     CDS             complement(4820..6847)
                     /locus_tag="PKL17_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNK44393.1"
                     /translation="MLAQLADVHFGYPGTEIFESLSFQVNPGQRIGLVGPNGAGKSTL
                     MRLLVGDLLPDRGQVVRARGKEISYLHQSQEFHGAGTLWETLLLPFARLLDMRAEIAK
                     LHDEIALLSHDEAESEKGRALLRRYGDLEHEYAHHDGYNLEVRARALAYDLGFSETDL
                     NRAVATLSGGERGRVELAKVLLAQPDLLLLDEPTNHLDVEAVEHLEERLKQWDATRAF
                     VVISHDRYFLQAVCNEIVDVEDGEVVRYPGSYEKYLVARQERHDMLNAAWQRQQEEIA
                     KTEDFIRRNIAGQKTNQAKSRRKMLDKVDRLKRHRDTWAEAGEIGLRFATSDHRGAKE
                     AIRTEHLCLGFGDDAPLLRDFSTTIYRGERIGIIGPNGAGKTTLMKALIGRGPQRGGQ
                     CYLGPEVRIGYFDQKLSDLDDDHSLIDEIRSIRGDWNEDKTRTYLGRFRFTGEDGFKK
                     VRGLSGGERNRLQLAKLMLKPVNLLVMDEPTNHLDIPARETLEEALCAFDGTLLVVSH
                     DRFFLDRVVTKLYHLDPASASIDTHIGNYSDWKRRLLEEKRAAQEQAEAAKAQAAKAA
                     AKGSAKPAQNKNAATEIPDESADKAKRIAEYEAKKERERQRDRKVKRLGQVEVDIAKL
                     EARLKELRETLTAEHGGDWQKLHKLVDEEHQTDRKLQSLMSEWETLSEELA"
     gene            complement(6918..7352)
                     /locus_tag="PKL17_06410"
     CDS             complement(6918..7352)
                     /locus_tag="PKL17_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44394.1"
                     /translation="MIFRSPPPKSGPLHVLEDPFFKATVNRQEHFLLITRSEKGFSDG
                     QEARLVHKNLVEAVAPYLRFRVIVDLRRAKGNSSNEIEKAITEVWFPTLIQFSPLAVL
                     VQTAAGRLQVTRMFREQGASGKGFLSVAEALRHIGLPEDHPL"
     gene            7563..8108
                     /locus_tag="PKL17_06415"
     CDS             7563..8108
                     /locus_tag="PKL17_06415"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK44395.1"
                     /translation="MADLCIAQTVPPQPVAKKRGYRPRGPVAVGTPSPSGSSPASPAA
                     DPGQPEAPPPPTVILRPEDKRQQSAGDKLNTTEVINMEDSHLSDRELQQEDFDAVFRP
                     RQGDILGCIDDARGDAQLEGQVAVAFRVQRTGKVSGVRVEAPAYLLDHGFLACVRKVV
                     QSLSFPVSNKSQVVTYPFALR"
     gene            8142..8819
                     /locus_tag="PKL17_06420"
     CDS             8142..8819
                     /locus_tag="PKL17_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNK44396.1"
                     /translation="MPLLLSLSMPGTRELFGYGVVALSAIFFVVDPIAVVPVFITITE
                     GDSAHKRKQMALRACIVTAGILLTFLIAGGLIFKLFSLTLAAFRIAGGLLLSLTGLDM
                     LRSVTSATKTSDKEVTEAVKKPDVAIVPLAMPLLAGPGSIATVMVLVAQAKHFWQVVI
                     LGVAIVITAAASYLMLRAASLVNRILGASGRAILERVMGLLLVAIAVQFIIGGVRDAF
                     PEIFVGR"
     gene            8928..9650
                     /locus_tag="PKL17_06425"
     CDS             8928..9650
                     /locus_tag="PKL17_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK44397.1"
                     /translation="MTQSQSAPPPVIDIREVSKVYRTSDVEVHALRSVDLRILRGEMV
                     AIMGQSGSGKSTLMNIIGTLDRPTSGSYHLDGVPVERLDETELARLRNRKIGFVFQSF
                     NLLPRHTALANVEVPLVYGRVPKIERSKRAAESLRRVGLGDRMDHHPNQLSGGQQQRV
                     AIARAMVTDPVLLLADEPTGALDTEMTDQIMKLFCELHKSGMTVVLVTHEPDVAAYAQ
                     RVVRFRDGRIVSDEQNPERPHA"
     gene            9643..11202
                     /locus_tag="PKL17_06430"
     CDS             9643..11202
                     /locus_tag="PKL17_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK44398.1"
                     /translation="MRSRQLAFSIGLATLALSSSAIAAPPPAADTVHLSLREAIELGL
                     KADPGVVSAKQSRDRADLAVTRSQLDRFSLRVDAFVNEQYRVSNIAGAAPTPTCSTLA
                     PVRSLTGSGNLYIPLQLLASSGGSLGAPSEADCMASGGAYYAGDSLQSGALGQFSLAA
                     NLNVPLFSGFRVTATVERAKLLRDAADASMHQSERQIALDVLRAYWSARRLELQLEVS
                     QQSLARFDEATAAVAARVRNGLAPVIDQNRMESRRQNEVSRRADLDGALLEAKAQLGV
                     LLGVSLASSSLSEPSELPPAPPESMESVDQLLTQARSQRPELRAAHANLLAQVQAVRI
                     ARSTYYPQIGLSGLLQFSNNPYNPLIGARVANSTANPFTNITGSVFLGSTLSLNLFDT
                     LNTYTAVRDAQLEEKRLLAEERRVGRAIEADVRVLHARLLHLYRMREPLLRSRALARD
                     NLSILERRYRSGDVAVLDLIDAATDLVTQEITLANQAATIAQTWGELYLAAGRLPPAS
                     LLAAQDLQGAR"
     gene            11221..>12254
                     /locus_tag="PKL17_06435"
     CDS             11221..>12254
                     /locus_tag="PKL17_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK44399.1"
                     /translation="MSEFNKKRSKWPTILAVMGVLLLIAGGVFWKSRAGKRAAFDPSL
                     LTKVTRGDLEVAVTELGKIEPREKVAVKSKVAGQVEKILIEEGMPVKSGQLLLFLDPT
                     EFQRSVVRAQQEVDKAQASLDFARVTLDRRRRAFADRAVAQADVDLAENDWKTKKIAV
                     AQAREALAVADDQLRYSRIIAPIDGTVIQRSIRVGETVVPGTMATLDEKSLMVVADMS
                     TLIAKVELNQIDVAKVRLDQDVTLTLDALPGKNYHAKVSKIAPAAITPKGKETDIFPV
                     EATLDAGALADIKPGMTADVKFHIDVRKGVLKLPIEAVTKEDGKHFVSKLVEKQPGQA
                     KVADKIEVKVG"
     assembly_gap    12255..12354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12610..13842
                     /locus_tag="PKL17_06440"
     CDS             12610..13842
                     /locus_tag="PKL17_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK44400.1"
                     /translation="MWLTYLQIALRVLRANMFRSTLTVASIVIGAFSIVLMTSLAESG
                     FGTLSRSFEELGGARLISMWPSKPEAAEGKDAMHLGGINRHDAEAVRSVPHLVALTQF
                     VSSWKQDLTSDEGVRVQGDLVGGDTSFLGFFRYQLSQGRGLDDTDITDRARTCVIGND
                     LAEKLWGKDAQVIGRYVTVAGTHCRIVGRLKKVDRWGMGFGWKWDEVLVMPIDTLLDR
                     IPKLGKEGRRMFLLTDSPTHNDIVKRIVNTVLLERHHGLDDFRLFDFETRLKGFFQVF
                     LIMKVIVGLLASISLVVGGVGIMNIMLVSVSERVREIGIRKALGATPADIGRQFLVEA
                     TLLSGVGGAFGAGLGILGALLGGAIITHFKPTWLTLISQPAVIVALTSSSLIGLVFGY
                     WPARSASRLDPVVAIRSQ"
     gene            13855..15114
                     /locus_tag="PKL17_06445"
     CDS             13855..15114
                     /locus_tag="PKL17_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK44401.1"
                     /translation="MSLFASLLDLLSSVLHSFRQHKVRAFLTLLGMIIGAGSVVLLSG
                     LLAGGEDALSSTQQFIEEKDVIEIENAAAPEKQRNRTQRALDYLDQRALDRSVATGGS
                     PTEGELMDWRHKAKVGETTKNVMVLGASPQALDLYHVKLEMGRFIDEHDLQKRARVAV
                     IGYEVWKDLLSGTRDLNGKSVTIGSVRWQVIGVLAHKPPMVAGPGTWMWDRRVVVPAT
                     TFQGVIRHSRKLDSIYIRVLPQLGDLMQAVVRARSWAKTTILSRHYEVENFKVDSDKD
                     SQQQAEVIFLVINILMLCTAALSLFVGGINIMNIMLVTVTERTREIGIRRALGATRGD
                     ILQQFLLEAGIIAGLGGMAGVLGGMALVFSISKILAYVLGSWTAHYKLWAILLGLGSS
                     TFTGVFFGLYPAWRAARLDPVEALRYE"
     gene            15199..15756
                     /locus_tag="PKL17_06450"
     CDS             15199..15756
                     /locus_tag="PKL17_06450"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNK44402.1"
                     /translation="MRFVHAALFALLLSASPALARPVASVEVPETLSFEGKTLKLNGA
                     GIRKKVIVKVYVGALYLEETTQNGDAIIKSDAVRVVKLTFLRSVEKDKLIGAFKEGFE
                     KNSADKAAAFAAHLEKLAAVLTDAKEGSVMTVAYVPGKGTQIGMQGGGSVTIEGKDFG
                     DAMFRNWLGAKPADGGLKTEMLAGK"
     gene            complement(15830..17347)
                     /locus_tag="PKL17_06455"
     CDS             complement(15830..17347)
                     /locus_tag="PKL17_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK44403.1"
                     /translation="MKRRWLLYVGLLSLLVIPALIPPSTTYDPTQLVAGLVESATPAT
                     KGPLQRPGSLEIKNVRLAGTEALTSIRIENGRITRIGGEPGSDLPILDGGGRYVMPGL
                     FDAHVHLSLAPGAALRSDDAKTSEALRAWHLRAYVASGVTSILDPAVDDTVAKDVRGK
                     LAQGHVGPRYFALGPPVAARGGYIDDLFPPGYSDAQQDGVASHMDRLVAMAADGVKVP
                     LEPGMLAPIWNVHSPQMLATIRREAEKRKLPIYVHAINDAMYQLGLPLAPHTFVHPPE
                     TISDGTLQRLIDSHAYVMSTLSVYDVQRFLLTPDEFSDPQGQLTIPPVEMETLRNPAM
                     QRRYLVDMIGENMPFVKGRYRDLFGIVSATKRGQNMMISALGPRLSATAKTVVKLHRA
                     GVPIVMGSDSGNWPVFPFFFHGPTTWREIRLLERAGLSPEETLRAATLEPARMLGIEN
                     DQGTISVGKRADLLLLDADPLKGLGKALPSLHTVVLNGIAHTKEEWMNLPSSFPK"
     gene            17476..18021
                     /locus_tag="PKL17_06460"
     CDS             17476..18021
                     /locus_tag="PKL17_06460"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNK44404.1"
                     /translation="MALTATVYHLPVALSDVDRGVYETLDLRLARHPSETMRYLLLRL
                     LAYCLLYEEGIAFSKGGLSSTDEAPISIRDRTGLLRAFVDIGFPSADRLHKAAKAAPR
                     VILFTAVEAHLLQKEARERTIHRVEEIEVWRIDPTFLDALEPLVNRHTKIDLLRSDGV
                     LYATVDGKTLETPLSRISLGA"
     gene            18129..19262
                     /locus_tag="PKL17_06465"
     CDS             18129..19262
                     /locus_tag="PKL17_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017651000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44405.1"
                     /translation="MAMRSWLLCSTVAFMLGAGCTEPTGTQPGGGSAGDKGEKGDKGD
                     PGLPGPKGEKGDKGDTGAAGPMGMTGPAGPMGMTGAKGDKGDPGAVGPTGPMGPAGTT
                     GATGPAGATGPAGATGPAGSKGDKGDRGDPGPQGPTGPAGTSAYAEDVASFAGYTTGT
                     TTGAITNGRYGMHALCAAAFPGSHLCHSSEYAFTNSAVNPPTDGAWIDPSFTIGISGV
                     RQLSGTSCDSWSQTASGYYGVYVTPSGGLVTTDCVKRKALACCNSVARTQFVGFTTAT
                     TSGAAGSRFKMHQTCAAEFAGSHLCHSIEYARSHSAVVPPANGAWIDPSNGNLAGNAR
                     DLAGTSCDNWTQMASGYYGVYVTPNGSLVSTDCVKVKSLACCR"
     gene            complement(19271..20056)
                     /locus_tag="PKL17_06470"
     CDS             complement(19271..20056)
                     /locus_tag="PKL17_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNK44406.1"
                     /translation="MTLPPAYAQVLGLGVLWTSIHCVGMCGPLLSGFDIAGVMRGRNR
                     WQGIGGVLLYQAGRGLTYAWLGSLAGLLGAGLSHAAQTGGAVLALLFGVLLCGMALRT
                     LRPNPSDAPLVKLRGGRSLSLSPAQVVESVLVRARQSLLPLLASRHPLRELALGAMMG
                     FLPCMIPAWALSQAALTGSPLHGALVMLLLVGLTTPILLVATQLPRLLLQLPPLLQKR
                     LPALLYLCSGMWLILVGGAGLALWPHAHLGFALFGRPLLLMLF"
     gene            complement(20058..21455)
                     /gene="ccoG"
                     /locus_tag="PKL17_06475"
     CDS             complement(20058..21455)
                     /gene="ccoG"
                     /locus_tag="PKL17_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNK44407.1"
                     /translation="MKREEPTLDKLHMLDERGSRQYIYPADVHGRFSKLRPLVFYILI
                     AVYISLPWITIGGHPAVYLDIAARRFFLFGRTFNAQDLYLAFFILTGIGFSLIVMAAL
                     FGRVWCGWACPQTVFLEGVFRRIERLIEGPASKRQALARGPWTAEKILRKAALHICYV
                     VIAYVLAHLFLAYFVSAKQLLRMIQDGPFAHVSTFLWGVGMTAVFYWNFYWFREQLCL
                     IVCPYGRLQSVMQDPDTVVIGYDKKRGEPRGKAHGPGVGDCVDCHRCVAVCPTGIDIR
                     NGLQMECVGCAGCIDACDEIMVKLGRPKGLIRYDSERGLLGEKRRFVRPRLYLYLLLG
                     MLGLVVSTTLFLRHTAFESNLLHARGMTFVIDGDIVRNQFVVHLINKNPVKSTLQIES
                     AATAPLTLIIPQPTVELESLASIEVPVIASIPRADYHAGIRVKLKVTDSASKTDRIMD
                     AALTGPTSPATGNSH"
     gene            complement(21546..22178)
                     /locus_tag="PKL17_06480"
     CDS             complement(21546..22178)
                     /locus_tag="PKL17_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNK44408.1"
                     /translation="MSAQEQGSTANAGAAPGQDVETNHDYDGIREFDNPLPRWWLLTF
                     YATIVFSFGYWFYYHTSQTGDLPMVAYQKELDEAKKAEDKRMAEQEAAGKGVTEEQLL
                     AMSKDAAVLERGAAVFKQNCLACHGAEGQGTVGPNLTDNYFLHGAKAKDTYQVIGTGV
                     LEKGMPAWKTVLGATKVQEATAFVLSLRGKHVAGKPPQGKTESGEPAPAQ"
     gene            complement(22182..22397)
                     /locus_tag="PKL17_06485"
     CDS             complement(22182..22397)
                     /locus_tag="PKL17_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44409.1"
                     /translation="MYHEFFSQKGVLVLPILAMLAFLLSFFAVVVATFRKKQASVFQS
                     IAQLPLGDDGEVKRAPVLPAGSQRKGV"
     gene            complement(22410..24716)
                     /gene="ccoN"
                     /locus_tag="PKL17_06490"
     CDS             complement(22410..24716)
                     /gene="ccoN"
                     /locus_tag="PKL17_06490"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNK44410.1"
                     /translation="MKEVRITYDDVTVRRFMLASIFWGIIGMIVGLLIASQLAFPELN
                     LASFLSFGRLRPLHTNAVIFAFVGNMMFAGVYYSTQRLCKSRMGSDLLSAIHFYGWQL
                     IIVCAALTLPFGFTQGKEYAELEWPIDILIALIWVVFAINFFWTLAIRKEKHLYVALW
                     FYISTILTVAVLHIVNSLALPLFSGLKSYSIYAGVQDALVQWWYGHNAVAFFLTTPIL
                     GIMYYFLPKAAERPVYSYRLSIVHFWALIFMYIWAGPHHLLYTALPDWAQSMGMLFSV
                     MLWAPSWGGMVNGLLTLRGAWGKLRTDPVIKFFIAGVTFYGMATFEGPLLSIKSVSGL
                     AHYTDWIVGHAHTGALGWNGFMAAGMFYYLVPKLWNRPLHSRSLADLHFWTATVGILL
                     YTISMWIAGVTQGLMWKAVNPEGALLYPSFVETLLAIRPMYLTRMVGGLLYITGFVLM
                     IVNLWKTISAGEAVETSITVVEEEESHKEVAWTAILFSRPLLLSVVALVGMAAFFLID
                     SVKIWIPTVALLTGFVLVMWFSYAMKKDDSGRLNVHRTLEGRSLIFTALVLIAVLIGG
                     VAELLPTILIDRAVPMTGAVQKPYRPLELHGRDLYVREGCYTCHSQMVRPFPSEKLRY
                     GDPSTAGEFIYDHPFQWGSKRTGPDLHRLGGKYGNLWHYQHLMDPRSTSQGSNMPPYA
                     FLAKSKLDLGDTAVKMNAMKTLGVPYSAAEIGNGNAELEAQAKAIAKDLAGQGITVDW
                     DSQMVALIAYLQRLGRSQGVAPLAQVTR"
     gene            complement(24713..24901)
                     /locus_tag="PKL17_06495"
     CDS             complement(24713..24901)
                     /locus_tag="PKL17_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase"
                     /protein_id="HNK44411.1"
                     /translation="MNALYLTLFVSLLLVIAAGILFGFLYTRRSHEYADRLSLLPLDS
                     DEEIKVSPSPSRTKERRS"
     gene            complement(24898..27423)
                     /locus_tag="PKL17_06500"
     CDS             complement(24898..27423)
                     /locus_tag="PKL17_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNK44412.1"
                     /translation="MTALSVCPHCNSPASSDAAYHPYCCVGCKAAHGLLQSAGLLNFY
                     ALRGATRLSAVGSRGEKRERLWLTPLLDQAESKAADAELVALRLDVQGLECAACVWLL
                     EKLFLKKPGAARIMVNPAVGRIELLYRRTQHGRQTVEQFVDDVEALGYRLGPARKDGD
                     AKVDDLVLRLGLSAALSMNSMIFAFSTYFGLSQREEPIIYKLFGYGGLALGTATVLIG
                     GTVFFRSAVQAISRGILHMDVPIALGILLTYVGSVWSFFAGEGRASYFDTLCIFITLM
                     LLGRLLQRRLASHNRQRLLADDTVSGLLVRTIRTGTDGTSRLEILPASQIQKDQVLLC
                     APGELLPVQATLLDGTAEFSLDWIMGESTPKQYAEKQAIPAGAHNRGKEAVRLSADEA
                     FSASRLRDLLCAPSVEKPLPGDVAASGQRADFWDYLSRYYVSLVLLLATTAFALWFRS
                     GVLRATEVAVAVLVVTCPCALGIATPLAYELAQARLRRRGLFVRTPSFFDRALGLRKV
                     LLDKTGTVTLSELTLANKAPLHGLSSEQRQALFQMVARSNHPVSRAILSAISQLGTED
                     GIDATAQVEEHAGLGLSLGRFGHTYRLGTPRFVLPTDADSQADSQSDSDSSEPQSVFG
                     CDGVPLARFSLSEALCRDAASEVSALKAQGLSLFILSGDRQDKVSRIGRQLGICDAFG
                     GLSPEQKCAMVKQIDDGHGDTLMIGDGINDALAFSAAACAGTPAIDRPTLPARADFYF
                     SGVGIGPISEALKTAKALRRTILRNLGLSSFYNLIVLSLSFAGLMTPLRCALAMPLSS
                     LVVIFATVRSLRDKDEATDQNIPHAPDEKSSETESPLLLGVSS"
     gene            27654..29195
                     /locus_tag="PKL17_06505"
     CDS             27654..29195
                     /locus_tag="PKL17_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK44413.1"
                     /translation="MQAPSLARHFHLLSDADLQALAPVLRHLREQRTRVLDEFYKRYL
                     IHFGQEVSLGFSDFSALYGRDLDAMVRHLADLDMDDFAAEVRAVGYEMSERGVPFAEV
                     ISSLHLLEEAVGTVLGPAVARGELQPRVFQIFDKLSHCRIIVLAGAYFTGQSADAEQQ
                     LHNVSSLQDGSDLARQFSSLVGQSEAMRRLFAQLQAAALSPGGAALLVGESGTGKELA
                     ARALHQKSRGVKRPFVAVNCAALPRDLIESELFGHRRGAYTGAQTEYGGLVRAASTGT
                     LFLDEITEMAVEVQAKLLRVLEEKAVRPVGAPGEIAVDVRFIASTNRDPQKAVAAGLL
                     REDLYYRISAFRVDLPPLRERRGDIPLLVRHFAELLHKRGLRAIEQFDAAALRALQTY
                     TWPGNVRELRNAIDFALTVGSGPALLRDDLPPHVLRGAAPPLPGDAPASGDSPSSAEA
                     SVPASDKDSAGAVPTMEEAERELIQRALSATGGNKLQAARMLGISRHRLYDSLRRFRI
                     EDAEA"
     gene            29283..29876
                     /locus_tag="PKL17_06510"
     CDS             29283..29876
                     /locus_tag="PKL17_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNK44414.1"
                     /translation="MGLYRKPADTRRSAPRAIRVAASDSAKDSASADSQGRSASRAPT
                     LYANMIEQHHEIDQLLSRLVQAVAHINGPPERAQARRLLTRLRKTLYAHISLEDGILF
                     REFESQSGLLLEGPTATLRREHQVLKARIEELSVSIDNGEKTPTLLRLLEAFCALYAD
                     HGRREEVMYGICERLLSPELLAKAQTLLRRKPSRSGD"
BASE COUNT         6021 a         8855 c         8889 g         6167 t
ORIGIN      
        1 gttttggctt ttgccagcgc acaatcaccg cttttcgcca gcgtcacgtc atagccacgg
       61 tcggaaagct ccgcctgtag gaagcgaagc aggtccacat cgtcatcgag tagaagtgct
      121 cggatactca cgctgcctcc gcctgaaaat agagccgcat cgtcgtcccg tgctcgccgc
      181 ctgacgccga ttctcccgct gtgcggcttg tcacttcgat gaagccaccg tgttcttgca
      241 caatttcgtg cgcaatcgac agcccaagtc cggtgccttc gctgacggct ttggtggtga
      301 aaaacgggtc aaaaatgttg gcgcggatct ctttggcgat gccgctgccg ttgtcgctga
      361 cctccagcac cgcccacttt tgcggcttgc cgccagggga cttgtcgctg cggacttcct
      421 cgcgaacggc gacgcggacg atgccgtctt cgccgactgc ttgcagcgcg ttgaccgtga
      481 tgttggtcag tgcctgttcg atgagcccga cgtgggcgat gcagcgaata tcggcacttg
      541 gcggcacaag cgaaagctcg cagttttttt tccgcgccac cggcgtaagc caagccgtga
      601 cgcgacgggc gacatcttgc agcgacaccg tgcttggctg accgctcggc ttgcgggcaa
      661 acgtgagcag ctgttcgacg atgatgcgca tccgttcgca ctggctgcgg atgctttcgg
      721 cttcggcgcg cacgtcatcg gaagcagcct gtgcggtcgc gatgcggttg gcgcgaccgg
      781 caacaatgtg cagcggcgtt cccagctcgt gcgcgatccc tgctgccagt gttccgacgg
      841 tggctagccg gtcgttgtgg cgcatttttt cgacgacggc gacccgcgct gcggcttcgc
      901 tttcgacccg ccgatgcagc tcgccgagcc gctcgaccat cgcgtccagc tcggtgctca
      961 gcagcgtgat ctctgcgctc gattcattgc cgatgaggaa tcgcacgcca aagtcgcctt
     1021 tgccgaccag ccgcgctttg gccacgatcc gattgagcgg tgccgagata agccgcgccc
     1081 ccagcagcac cgcgacgatc acgccgacca gcaccgtcag tccggtgttc ttgataaacg
     1141 tgcgcaggtt ttcgctcaca aactcggact ctccttgcat ggcccgtcgc agcgtcacgc
     1201 caaatcgctg tccggcctcc tgtccgagcg acagccgatc gaacgccacc agcaccggcg
     1261 gacttttttg gtggtcgatg aaaatcacct gctcacccgc ttgcgcttgc tttcgcagct
     1321 cggtaggaaa cgccgcgaac agatcgtctt ttgacagggc atcgagccgg accagatgca
     1381 ccgaagaata atcttgccgc gccgccaccc gatcgagata gcggcgcaga ctcatctcgc
     1441 caccgtcgtg ccatgccagg tttgcgcctt catcaagcgc ggtcaaaagc gcttgcagct
     1501 gctcggtccg catctcttcg tagcgccact cgaagcgacc cttttgttcc caggtaatcg
     1561 ccgacagtgc cagcacaaag gtaaagatga tgagcatcgt gagacggagc gcgagtcgca
     1621 tgggcgtgta tccttgagcc cgcaggtgac aaacaatgat gcgctgccac cgtgcttgcc
     1681 gggccgtaga ctgcgttgcg atgtagcttc tatgcaagcg ctgcgccatc gactctgcga
     1741 cgcagcgaag ggagtgcgga ctctttccgt gcggaggaac tccccagcct ggggaaactt
     1801 cgggctccta gtgttcacac aggctgacgg cgcgcacgtt gagcgactgt ttgtgacgct
     1861 tgcgctcttc catcagctcc agccttaccg cgagcgcttc gtctttcgga atcactgttt
     1921 gctgtggcgc aacttcccgc gtcgacacaa gccgcacatt gggatcgacc acccagaaca
     1981 caatcacgcg acgacggcct tcacttgatc cagagacaag cgaaagctcg ctgagcttgt
     2041 gaatgtgtga attgggaaac accagcagac gaccttccgg agtctccaga ctgccgagcg
     2101 gaatcgttcc ttcgtctacc agttgttcga tcggacgggg ccgacactga tcaatgttcc
     2161 agaacagacg acctgcttct tcgacggtgt aggctcgctt gaaacgcagt gcgccgcctt
     2221 ggatcgccgc gtcgcgatcc agaatgtaca cgcaggtcgc gacgatgtgt tcatggctca
     2281 ttccttcgac gtgccagacg ccttcatgtg tttccccttg tccgagccgg tattcgacaa
     2341 tcttgggaat cacttgcagt tcacgtccgc gcagcgacgt tggcttcacg gtctgggctt
     2401 cgtcaccacc ggattccgga agctcgcctt cgtgctcgat ttcatcgcac tcttcttgcc
     2461 agaacttggt gctgtccacg tatcctgtca cgctttcaac aagcggaagc gccgttgcaa
     2521 acagcgcagc cagatcgtca taaagcgccg gatattttgt gcgatccagg ttgtttatgt
     2581 actctggaat ctggattttt ccgtcgctgc ctatcgcaaa cggagtcggg agccactgat
     2641 agcgcgacga ttcaaacgga cgaccaaacc gatcataggg cggcgattcc gctttgggaa
     2701 cactgccaac caccaccgat ttcccttcga tgtacggata caaagacgga tggaccagat
     2761 cgcgaacttt ggttcccgaa cccggatggt aatcaaccgg ttcttcgtcg ataagtcgct
     2821 tcacattggc gcgcagtcga ccgaccaaat tcgcatcgac caagccgtca ccatacgcga
     2881 ttccgtctac acatggcctg cgcaggtgct gttcccagat gttctggatc tgcggcgatt
     2941 caacaatccg gaaccagcca tccgacgaca cttccatttc ctggccgctc tgctggtctt
     3001 tgagtgtgag cgtttcatcc ccggatgcaa ccaccagcgc ggaatcggaa gggaagggct
     3061 ctccgctggt ggtttcaagg tcgcccggct caaactcgac gatgtcaccg ggctgtggcg
     3121 ggtgcccctc gccatatgtc aaaagctcct caacaaatgc aaagtcgctg ctttgggcaa
     3181 agtgcggggc agactgcgcg ctcgacatcg gatctccttt ggcttctggc ttcaaactag
     3241 cgcagcgcgc ggacaaaagg aatcttgtag gtgcgctgcc tggaaaaatg cgctgacatt
     3301 tcgtggcgat ggcacacgcc tctacacagg ccagccttgg cagcgacttg gcggcgcgat
     3361 accatggggc gtgaactcat ctgaacttgc gctgctccat gcatcccttg ccgagctgtc
     3421 cgatctcgtc gccttttcga gtgtgctgta tcggaaaatg ttcgagcgtc agccaagcta
     3481 tcgtgcgctg tttgccgcag acctggaacc acagcttacg cggatggctg cggcgattcg
     3541 ttttggcatc ttgcacattg cggacgatac ggtgcttttg ccgctgctga cttcgtttgc
     3601 ggcgcgtcat gtcgaagccg gaatcaccaa tcagatgttc gaagtcgtcg gtctggcgct
     3661 gcaagatgcg ctaaaggaac agcttggaga gagattttcc gatccactcc cacggctgtg
     3721 gcagagcgcg tatcagaagc tgtcgtgggc actgcaagaa cggatgtggc atgcgccgca
     3781 agatggcctg ccgcagatgg agcgcgcgat tcgggaaaag ctgctgctgt catcgtatga
     3841 tgatcttccg gcggtcgcgc cgctccatcc ggttcacgat gactgtacgg tgcagtttgt
     3901 cggtgtggga aaagtaacgg tgcgacgggg ggacacactc ctttcggtgg cgcgcacgca
     3961 tgacattccg atgcttggtg aatgtggcgg gcatggtcgc tgcacgacct gtcgggtgtt
     4021 tgtcgaagaa ggaatggagt ggcttggtcc acgcaccgta gccgaggaag cgattgctca
     4081 gcgtcggcat ctgccaccca gagtccgtct cgcctgtcaa accaacgtgc tgggtccgtg
     4141 tcgggtgcgg cgactgcttc acgatgaacg cgacattgct gcggtgctgc ggcggcgttc
     4201 cggaatcagc ggtcagcagc gcgagattgc gattctgttt gccgacattc gcgacttcac
     4261 gccgtttgtc gaacagaact tggcgcacga tgtgacgcac atgctcaatc gctatttcac
     4321 cgcgatcgtg gatgcgatgg agccgcacgg agcctacgta gacaagtaca ttggcgacgg
     4381 aatcatggtg ctgttcggac tggattccca gcgccatgtc cacccttgtt tggatgccgt
     4441 gcagtccgcg cttgcgattc agcgcgcagt gtcagaacag aatccgtact tcgaaaaata
     4501 cttcggacac cgctttgtct gtggcgtggg agtccacttt ggacccgcag tcatcgggga
     4561 cattggatcg tggcggaagt cgcagcttac ggcgcttggc gataccgtca atgtggcggc
     4621 gcggatcgaa tcagcgacga aacagcttgg gcacgcgatc ctggtttccg aacccgtggt
     4681 gcgtgcgctg cacaagctgc cgtccgatct tccggcagcc aaatcgtttg tcatcggaga
     4741 gtctgtggaa gccaagctca aagggaaaag cgagccgcaa aacgtctatc cagtccggcc
     4801 tgcctagacc gcggattcct tacgccagct cctcactgag cgtttcccat tcgctcatca
     4861 gcgactgaag tttgcggtct gtctgatgtt cctcatcgac cagtttgtgc agcttctgcc
     4921 agtcgccgcc atgctctgcg gtcagcgttt cccgcagctc tttgagccgc gcttccagct
     4981 ttgcgatgtc gacttcgact tgcccaagcc gcttcacttt gcgatcgcgc tggcgttcgc
     5041 gttccttttt ggcttcatat tcggcaatgc gcttcgcttt gtcggcagat tcatcaggga
     5101 tttccgtcgc tgcgttcttg ttttgcgcag gctttgcact gcctttggct gccgctttgg
     5161 ctgcttgcgc tttggccgct tccgcttgtt cttgcgccgc gcgtttttct tccagaagcc
     5221 gccgtttcca gtccgaatag ttaccgatgt gcgtatcaat cgacgctgac gccgggtcca
     5281 ggtgatacag cttggtcacc acgcgatcga ggaagaaacg atcgtgactg accaccaaga
     5341 gcgtgccatc aaacgcgcac agcgcttctt ccagcgtctc acgcgcggga atgtccaggt
     5401 ggtttgtcgg ttcgtccatc accagcagat tcaccggctt taacatcagc ttcgcgagct
     5461 gaagccgatt ccgttcgcct cctgataggc cgcggacttt tttaaaacca tcttctccgg
     5521 tgaaacggaa tcgacccaaa tacgtgcgcg ttttgtcttc gttccagtcg ccccgaatgg
     5581 atcgaatctc gtcgataagc gagtgatcat catccagatc agacagcttc tgatcaaagt
     5641 atccgatccg cacttccgga cccagatagc actgaccacc gcgctgcgga cctctgccta
     5701 tcagcgcttt catcagggtc gtttttcccg cgccgtttgg tccaatgatg ccgatccgtt
     5761 cgccacgata aatggtggta ctgaagtcgc gcagaagcgg tgcatcgtcg ccaaaaccca
     5821 aacagaggtg ctcggtccga atcgcttctt tggccccccg atgatcactt gtcgcaaagc
     5881 gcagaccgat ttctcccgct tccgcccagg tatcgcggtg tcgcttgagc cggtctactt
     5941 tgtcgagcat cttgcgccgg ctcttggctt ggttggtctt ctgtccggca atgttgcgac
     6001 ggatgaagtc ttccgttttg gcaatctctt cctgttgcct ctgccacgcg gcgtttagca
     6061 tgtcgtgccg ttcctgccgc gccaccagat acttttcgta gctgccggga tagcgcacca
     6121 cttcgccatc ttcgacatcg acaatttcgt tacagacagc ttgcaggaag tagcgatcgt
     6181 ggctgatcac cacaaacgcc cgcgtcgcat cccactgctt gagccgttct tccaagtgtt
     6241 cgaccgcttc cacatccaag tggtttgtcg gttcatcgag caagagcaag tccggctgtg
     6301 ccagcagcac tttcgccagc tcgacacgac cccgctcgcc tcctgacagc gtcgccacgg
     6361 cgcgattcag atcggtttcc gaaaagccca gatcataggc cagcgcgcgc gcccgcactt
     6421 ccaagttgta gccatcatgg tgcgcgtatt catgttccag atccccgtaa cgacgcagca
     6481 gcgcccggcc tttttcgctc tctgcttcgt catgggaaag cagtgcgatc tcgtcgtgca
     6541 gcttggcaat ttctgcccgc atgtcgagca gcctggcaaa cggcaaaagc agcgtttccc
     6601 acagcgttcc tgcgccatgg aattcctgcg actgatgcag atacgaaatc tctttgccgc
     6661 gcgcgcgtac cacttgcccg cgatccggaa gcagatcacc gacgagcaaa cgcatcagcg
     6721 tcgattttcc ggcgccgttt ggaccgacca gaccaatccg ctggccgggg tttacctgaa
     6781 acgacagact ttcaaagatt tccgtaccgg gatagccgaa gtgaacgtcg gcaagctgag
     6841 caagcatggc cgccgattct atcgcgccgc cgctcccatc gcgcacaaat tgggcagccc
     6901 gcttctcaaa ctgcggctta cagcgggtga tcttcgggca agccaatgtg acgcagggct
     6961 tcggccaccg acagaaagcc ctttcccgaa gcaccttgct cgcgaaacat ccgcgtaacc
     7021 tggagccgtc ccgccgccgt ttggaccagc accgccagcg gagaaaactg gatcagcgtc
     7081 ggaaaccaga cttcggtgat cgctttttcg atctcgttgc tcgagttgcc cttggcccgt
     7141 cgcaggtcga ctatcacccg gaagcgcaga tacggagcca ccgcttcgac caaatttttg
     7201 tgcaccagcc gcgcttcctg gccgtcggaa aacccttttt cagatcgtgt gatcagcaga
     7261 aagtgttctt gccggttcac cgtcgcctta aaaaacgggt cttcaagaac atgcagcggc
     7321 cctgactttg gcggggggct tcggaagatc atgtaccagt cgggtacaaa catcgtatca
     7381 cgtcgacgag attcggtcgt gatacttgcc agcgcggggg gttttagaaa actcttctat
     7441 ccatgcgcgg ttttttgttt ggcttccttg tgtgtgcgct gctctgtgca ggggcatacc
     7501 atcagtattt gcgaccacag ccgctggacc cgtgccgggt ttgctcgacg ggaactcgct
     7561 gcgtggccga tctttgcatt gcgcagacgg tgccgcccca gccggtcgcc aaaaaacgcg
     7621 gttatcgtcc gcgcggacct gttgccgttg gcacgcccag tcccagcggc agcagcccag
     7681 cgagtccagc cgcggacccc ggacagcctg aagcgccgcc gccgcccacg gtgattttgc
     7741 gcccagaaga caagcggcaa cagtcggcgg gcgacaagct gaacaccacc gaagtcatca
     7801 acatggaaga ctcgcacttg tccgatcgtg agctgcaaca ggaagacttc gacgcggtgt
     7861 ttcgtcctcg gcagggcgat atcttgggct gtattgatga tgcgcgcggc gatgcccagc
     7921 tcgaaggaca ggtcgccgtc gcttttcgcg tccagcgcac cggcaaagtc agcggcgtgc
     7981 gggtcgaagc gccggcttac ctgctcgatc atgggttttt ggcctgtgtc cgcaaagtcg
     8041 tccagagcct gtcgtttccc gtctcgaaca agtcacaggt cgtgacctac ccctttgcgc
     8101 tgcgctaaaa ctcgggtaac tttcgtcgcc gcggacgccc tatgcccctt ctgctttcgc
     8161 tttcgatgcc cggtacgcgg gaactttttg gctacggcgt cgtcgccctg tccgccattt
     8221 tcttcgtcgt cgatccgatc gcggtggtgc cggtgttcat taccatcacg gaaggtgact
     8281 cggcccacaa gcgcaagcaa atggcgctgc gtgcttgcat cgtcaccgcc ggcatcttgc
     8341 tgacgttcct gatcgctggc ggactgatct ttaagttgtt ttcactgacg ctggcggcgt
     8401 ttcgcatcgc tggcggactt ttgctgtcgc tcaccggact cgacatgctg cgcagcgtga
     8461 cttcggcaac caagaccagc gacaaagaag tcaccgaagc ggtcaaaaaa cccgatgtgg
     8521 cgatcgtccc tttggccatg ccgcttctgg ctgggccggg ttcgattgcg acggtgatgg
     8581 tgctggtcgc gcaggccaaa cacttctggc aggtggtgat tttgggcgtc gcgatcgtga
     8641 tcaccgcggc ggcttcgtat ctgatgctgc gcgccgcctc gctcgtgaac cgcatcttgg
     8701 gcgcgtcggg gcgggcgatc ctcgaacgtg tgatgggcct tttgctggtg gccatcgccg
     8761 tgcagttcat cattggcggc gtgcgcgacg ccttcccaga gatttttgtc ggtcgatagg
     8821 tcgtttttcc ggaagtgttt ttcacccccg gctgttatct ccacgcacat agactgccac
     8881 gctgcctgct ttgcgcagcc gcaatccgaa gcaaggacat ctgtttcatg acccaatccc
     8941 aatcggcgcc cccgccggtc atcgacatcc gtgaggtcag caaggtctat cgcacctccg
     9001 acgtcgaggt tcacgctctg cgcagtgtcg atctgcgaat tctgcgcggg gaaatggtcg
     9061 ccatcatggg ccaaagcggc tcgggcaaat cgacgctgat gaacatcatc gggacgctcg
     9121 atagaccgac ttcgggcagc tatcacctgg atggcgttcc ggttgaaagg ctcgatgaaa
     9181 ccgagcttgc ccgtctgcgc aaccgcaaaa tcggctttgt ttttcagtca tttaacctgc
     9241 tgcccaggca caccgcgctt gccaatgtgg aagtgccgct cgtctacggg cgcgtgccga
     9301 aaatcgaacg ctcgaagcgc gccgccgaat ctttgcgacg ggtcgggctc ggcgatcgaa
     9361 tggatcacca tcccaaccag ctttcgggtg ggcagcagca gcgggtggcg attgcgcgag
     9421 cgatggtcac cgatccggtg ctgctgctcg ctgacgaacc aacgggcgca ctcgataccg
     9481 aaatgaccga tcagatcatg aagttgtttt gtgagcttca caaaagcggc atgaccgtcg
     9541 tgctggtgac tcacgaaccc gatgtcgcgg cgtatgccca gcgggtggtt cgtttccgcg
     9601 acggacgaat cgtatctgat gaacaaaatc cggagcgtcc gcatgcgtag tcgtcagttg
     9661 gctttttcga tcggattggc gacgctggcc ttgtcttctt cggcgattgc ggcaccgccg
     9721 ccggctgcgg acacggtaca tctgtcgctg cgtgaagcca tcgaactggg tctgaaagcc
     9781 gatccagggg tggtgtcagc gaagcaatcg cgcgatcgcg ctgatctggc ggtgacgcgg
     9841 tcgcagcttg atcgtttttc gctgcgcgtc gatgcgttcg tcaatgaaca gtaccgggtc
     9901 agcaacatcg ccggggccgc accaacgccg acatgttcga cgctggcgcc cgtgcgcagc
     9961 ctgactggca gtggcaatct ctacattccg ctgcagcttt tggcgtcgtc gggtggcagc
    10021 cttggcgcac cttcggaagc ggactgtatg gcatccggcg gggcgtatta cgcgggcgat
    10081 tcgctgcaat cgggcgcgct gggtcagttt agcttggcgg ccaacctaaa cgtgccgctg
    10141 ttttccggtt tccgtgtcac tgccaccgtc gagcgggcca agcttttgcg tgatgcggca
    10201 gatgcatcca tgcaccaaag tgaacggcag atcgcgcttg atgtgctgcg ggcgtactgg
    10261 agcgcgcgac ggctggagct acagctagaa gtttcgcagc agtcgctggc ccgctttgat
    10321 gaagccacgg ctgccgtcgc tgcccgagtg cgcaacggtc tggccccggt gattgatcaa
    10381 aaccgcatgg agtcgcgtcg gcaaaatgaa gtctcgcggc gggccgatct ggacggcgcc
    10441 ctgctggaag ccaaagccca gcttggcgtg ctgctgggtg tatcgctggc ctcgtcgtcg
    10501 ctgtcagaac ctagcgagct gccgccggcg ccaccggaaa gcatggaaag tgtcgaccag
    10561 ctcttgacgc aggcgcgcag ccagcggccc gagctgcgtg ccgcgcatgc gaacctgctc
    10621 gcccaagtgc aggcggtgcg gattgcgcgc tcgacgtact atccgcagat cgggctgtcg
    10681 ggacttttgc agttttcaaa caacccgtat aacccgctga tcggtgcgcg cgtcgccaac
    10741 agcacggcga atccgtttac caacatcacc gggtcggtgt tcctgggttc gacgctgagc
    10801 ctgaatctgt ttgataccct gaacacgtat acggcggtgc gcgacgcgca gctggaagaa
    10861 aaacggctgc tcgcagagga aagacgggtc ggacgcgcga ttgaagctga tgtgcgggtg
    10921 cttcacgcgc ggctgctcca cctgtatcgg atgcgcgaac cccttttgcg cagtcgggcc
    10981 ctggcgcgtg acaacctgag catccttgag cgtcgctatc gcagcggcga tgtggcggtt
    11041 ctggatctga ttgatgcagc gacggatctg gtgacccaag aaatcacgct ggcgaaccag
    11101 gcggcgacaa tcgcgcagac ctggggtgag ctgtatctgg cggcgggacg actgccaccg
    11161 gcttcgcttc tggcagccca agacctgcaa ggcgcccgct aacctgggga agagagtgag
    11221 atgagcgagt tcaataagaa acgttcgaag tggcccacca tcttggctgt gatgggtgtg
    11281 ctgttgctca ttgcgggcgg cgtgttttgg aagagccggg caggcaagcg ggcggcgttc
    11341 gatccgtcgc tgctgaccaa ggtgacgcgc ggcgatctag aggttgcggt gaccgagctt
    11401 ggcaagatcg aaccgcggga aaaagtcgcg gtgaagtcga aagtcgccgg ccaagtggag
    11461 aaaattctca tcgaggaagg catgccggta aagtccggcc agctgcttct gtttttggac
    11521 ccgaccgagt ttcagcgcag cgtggtgagg gcgcagcagg aagtcgacaa agcccaagcc
    11581 tcgctcgatt ttgcgcgggt cacgctggat cgcaggcggc gtgcatttgc cgatcgcgcg
    11641 gtcgcgcagg ccgatgtgga tctggcggaa aacgactgga agaccaaaaa aattgcggtg
    11701 gcacaggcgc gggaagcgct cgcggtcgct gatgatcagc ttcgttattc gcgcatcatc
    11761 gcgccgattg acggaacggt gattcagcgc agcattcgtg tcggtgaaac ggtcgttcca
    11821 ggcacgatgg caacgctgga tgaaaagtcg ctgatggtgg tcgcggacat gtcgacgctg
    11881 atcgccaaag tcgaactcaa ccaaattgac gtggccaaag ttcggctgga tcaggatgtg
    11941 acgctgacgc ttgatgcgct accggggaaa aattaccacg cgaaagtgtc caaaattgcg
    12001 ccggcagcga tcacgcccaa aggcaaagaa accgacatct ttcccgtcga ggcaacgctg
    12061 gatgcggggg cgcttgcaga catcaaaccg ggcatgaccg ccgatgtgaa gttccacatc
    12121 gacgtgcgca aaggcgtgct gaagctgccc atcgaagcgg tgaccaagga agacggcaaa
    12181 cactttgtca gcaagctggt cgaaaaacag ccagggcagg ccaaagtcgc cgacaagatc
    12241 gaagtcaaag tcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgaaa
    12361 aacagccagg gcaggccaaa gtcgccgaca agatcgaagt caaagtcggg gttcgcaatg
    12421 atcgcgaaca ggagatcgtt tcaggggtcg cagagtcgga cgaagttgtg atccgtccgc
    12481 cgtccgcggc tgccaacgag ttcaaataag gagccgtggg tctgcctctg cctggcgcga
    12541 cacgcggggc tttccgtttc tttggatttt cttttttcgg ttcgctcaca gcaaacagga
    12601 gccggtccgg tgtggctgac gtatttgcag attgcgctgc gggtgctgcg ggcgaacatg
    12661 tttcgctcga cgctgacggt cgcgtcgatt gtcattggtg cgttttctat cgtgctgatg
    12721 acatcgctgg cggaatcggg ctttggcacg ctgtcgcgct cgtttgaaga actgggtggg
    12781 gcgcgcctga tttcgatgtg gccgtcgaaa ccggaagccg ccgaaggcaa agacgcgatg
    12841 cacttgggcg gcatcaaccg gcatgatgca gaggcggtgc gcagtgttcc gcacctggtt
    12901 gcgctgaccc agtttgtttc ctcttggaag caggatttga catccgatga aggcgtccgt
    12961 gtgcaaggcg acctcgtcgg tggggacacc agcttcctgg gttttttccg ctatcagctt
    13021 tcgcaaggcc gtggactgga tgacaccgac atcaccgatc gggcgcgcac ttgtgtgatt
    13081 ggaaatgatc tggcggaaaa gctatggggg aaagatgcgc aggtcattgg gcgttatgtc
    13141 accgtcgcag gaacccactg ccggattgtc ggtcggctca aaaaagtcga tcgctgggga
    13201 atgggttttg gttggaagtg ggacgaagtg ttggtgatgc cgattgatac gctgctcgat
    13261 aggattccca agctgggcaa agaggggcga cggatgtttc tgctcaccga cagtccgacg
    13321 cacaatgaca tcgtcaagcg gatcgtaaac acggtgcttt tggaacgtca tcatggactg
    13381 gatgactttc gcttgtttga ttttgaaacg cggctcaaag gttttttcca agtcttcctg
    13441 atcatgaaag tgattgtcgg actgctcgcg agcatctcac ttgtcgtcgg tggtgttggc
    13501 atcatgaaca tcatgctggt gtcggtttcg gaacgtgtgc gcgagatcgg aatccgcaaa
    13561 gcactgggcg caacaccagc agacatcggg cggcagttct tggtggaagc gacgctgctc
    13621 tctggggtcg gcggggcgtt tggcgcggga ctgggaatct tgggggcgct gctcggcggt
    13681 gcgatcatca cccacttcaa gccgacgtgg ctgacgctga tttcgcagcc cgcggtgatt
    13741 gtggcgctga ccagctcatc gctgattggt cttgtgttcg gatactggcc ggcgcgaagt
    13801 gcaagtcggc tcgatccggt ggtcgccatc cgcagtcaat aagaggagta taagatgtct
    13861 ctatttgctt cactcctcga tctgctttca tcggtgctgc attcgtttcg gcagcacaaa
    13921 gtgcgggctt ttctgaccct gctcggcatg atcatcggcg ccggttcggt ggtgcttttg
    13981 tcgggacttt tggccggtgg ggaagacgcg ctgtcgtcga cgcagcagtt tatcgaagaa
    14041 aaagatgtga tcgaaatcga aaacgcggcg gcaccggaaa agcagcgcaa tcggacccag
    14101 cgtgcactcg attatctgga tcagcgggcg ctcgaccgca gcgtggcgac gggtggttct
    14161 ccgacggaag gagagcttat ggactggcgc cacaaagcga aagtcgggga aaccaccaaa
    14221 aatgtgatgg tgcttggggc gtctccgcaa gcgctcgacc tgtatcacgt caaactggaa
    14281 atgggccgct ttatcgacga gcacgatctg caaaagcgcg cccgtgtggc cgtgattggg
    14341 tacgaagtct ggaaagacct gctttcgggg acgcgcgatc tgaatggaaa gtccgtgacg
    14401 attggatcgg tgcgctggca ggtcatcggc gtgctggcac acaaaccacc gatggtggcg
    14461 gggccaggaa cttggatgtg ggatcgccgc gtggtggttc ctgcgacgac gtttcaaggg
    14521 gtgattcggc attcgcgcaa gctcgacagc atctacattc gggtgttgcc gcagcttggg
    14581 gatctgatgc aggcggtggt gcgggcgcgg tcgtgggcga aaacgacgat tttgtcccgt
    14641 cactatgaag tcgaaaattt caaagtcgat tccgacaagg attcccaaca gcaggccgag
    14701 gtgatctttt tggtcatcaa catactgatg ctgtgcacgg cggcactgtc tctgtttgtc
    14761 ggcggaatca acatcatgaa catcatgctg gtgacggtga cagagcggac gcgcgaaatc
    14821 ggaatccggc gagcccttgg tgccacgcgc ggcgacatct tgcagcagtt cctgctcgaa
    14881 gccgggatca tcgcaggact tggtgggatg gccggtgtgc tcgggggcat ggcgctggtg
    14941 ttttcaatca gcaaaatttt ggcttacgtg ctgggaagct ggacggcgca ctacaagctg
    15001 tgggcgattt tgctcgggct tggttccagt acgttcacgg gtgtgttctt tggtctgtat
    15061 ccggcgtggc gggccgcgcg tcttgatccg gtggaagccc tgcgttacga ataagcaccg
    15121 tcggtggcga aaattttcgc gcagtggtta cactttcgcg cgtacttctg cgagcatgtt
    15181 cagttaggag gaaagcgaat gcggtttgtc catgcggctc tgtttgctct tttgctgtcg
    15241 gccagcccag ccctggcgcg tccggttgcc agtgtggagg ttcccgaaac cctgagcttc
    15301 gaagggaaga cgctcaagct caacggcgcg ggaattcgca agaaggtcat cgtcaaggtg
    15361 tatgtcggcg cgctgtatct ggaagagacg acgcaaaatg gcgatgcgat catcaagtcg
    15421 gatgcggtgc gggtggtcaa gctgacgttt ctgcgcagcg tcgaaaagga caagctgatc
    15481 ggcgccttca aggaaggctt tgagaaaaac tccgcggaca aagcggcggc gttcgcagcc
    15541 catctggaaa aactggcagc ggttctcacc gacgcaaaag aaggatcggt gatgactgtg
    15601 gcctacgttc cggggaaggg cacccagatt ggcatgcagg gtggcggctc ggtcacaatc
    15661 gaaggcaaag acttcggtga tgcgatgttc cgcaactggc ttggtgccaa gcctgccgac
    15721 ggtggactga agaccgaaat gctggccgga aagtagtctg ggcgcgctcg gttgtcgctc
    15781 tgcacgttgg tccggttgat tcctgctgct tgtttgattc cctcgcacat cacttcggaa
    15841 acgaactggg aaggttcatc cattcctctt tggtgtgggc gattccgttc agcaccacgg
    15901 tgtgaagcga gggcagggct tttcccaacc ctttgagcgg atcggcatcc agcagcagca
    15961 ggtccgcgcg ctttccgacg gaaatcgttc cctgatcgtt ttcgattccg agcatccgcg
    16021 ccggttccag ggtcgccgct cgcagcgttt cttcggggct cagtccggct ctttccagca
    16081 gccggatctc tcgccaggtc gtcggtccgt gaaagaaaaa gggaaacacc ggccagtttc
    16141 cggaatcgct tcccatcaca atcggaacgc ccgcgcggtg cagcttgacg accgtcttgg
    16201 ctgtggccga caagcgcgga cccagcgccg agatcatcat gttttgccct cgtttcgtcg
    16261 ccgagacgat tccgaacaag tcgcgatagc ggcctttcac aaacggcatg ttttccccga
    16321 tcatgtcgac cagatagcgt cgctgcatcg ccggattccg cagtgtttcc atttcgacag
    16381 gcggaatcgt gagctgtcct tgcggatcgg aaaattcatc cggcgtaagc aaaaaccgct
    16441 gcacatcgta gaccgacagc gttgacatca cataggcatg ggaatcgata agtcgctgga
    16501 gcgtaccgtc ggaaatcgtc tctggtggat gcacaaacgt atgcggcgca agcggaagac
    16561 caagctggta catcgcatca tttatcgcgt gaacatagat cggaagcttg cgcttttccg
    16621 cttcacgtcg aatcgtcgca agcatctgtg gactgtgtac gttccaaatc ggcgccagca
    16681 ttcccggctc cagcggaact ttcacaccgt cggcagccat cgccaccaag cgatccatgt
    16741 gggacgcgac gccatcttgc tgcgcatccg aataaccggg aggaaacaga tcatctatgt
    16801 atcctccgcg cgcggccacc ggagggccca gggcaaagta gcgcggtccg acatgacctt
    16861 gcgccagttt tccacgcacg tctttggcca cggtgtcatc gacggcaggg tccaaaatgc
    16921 tggtcactcc ggaagcgaca tacgcgcgca gatgccacgc ccgcagcgct tcgctggtct
    16981 tggcatcatc gcttcgcagt gcggctccgg gggccagcga caaatggaca tgcgcgtcga
    17041 acagaccggg cattacatag cgcccaccac cgtcgagaat cggcaagtca gatcccggtt
    17101 ctccgccgat tcgggtgatg cgtccgtttt caatccgaat cgatgtcagc gcctctgttc
    17161 ctgcgagccg aacgttcttg atttcgagcg atccgggccg ctgaagtgga ccctttgtcg
    17221 cgggtgtggc cgactccacc aatccggcca ctagctgcgt cggatcatag gtcgtacttg
    17281 gcggaatcag cgcaggaatc acaagcaaag acaagagtcc tacgtacaac agccagcgac
    17341 gtttcattga aaactccgta ccccatgatc gccgaatctt tccgctggag caagccgcgg
    17401 ccatggcggg attccctagc gcggtctttt ttgcgctcgc gtttgttggc caaggtgtgc
    17461 gatgacttgg cactgatggc actcacagcg acggtctatc accttccggt tgcgctttcc
    17521 gatgtcgatc gcggcgtcta tgaaacgctc gatttgcggc ttgcgcgcca tccttcggaa
    17581 acgatgcgct atctgctctt gcggctcttg gcgtactgtc ttttgtacga agaaggaatc
    17641 gcgtttagca aagggggtct gtcttccacg gacgaagcgc cgatctcgat tcgggatagg
    17701 acgggtcttc tgcgtgcgtt tgtcgatatc ggttttccgt cggcagatcg acttcacaaa
    17761 gccgccaaag cggcgccgcg ggtgattctg tttaccgcgg tagaagcgca tcttttgcaa
    17821 aaggaagcgc gcgagcggac gattcacaga gtcgaagaga tcgaagtctg gcgaatcgat
    17881 ccgacgtttt tggatgcgct ggaaccgctg gtgaatcgtc acaccaagat cgatctgctg
    17941 cgatcagacg gcgttctgta tgcgacggtg gacggcaaaa cgctggagac accgctgtcc
    18001 cgcatcagct tgggagccta gggtttttta tgcccgtgat ggtgtcgatt gactcccaaa
    18061 gtgcctgcga tccatagtcc gcgcgtgtgg caaaaacctg gcccacaaaa caagcgaggt
    18121 acacggtgat ggcgatgcgg tcttggctgc tgtgctcgac ggtggctttc atgctgggcg
    18181 cgggttgtac ggaacctacc ggaacgcaac cgggcggcgg atctgcgggg gacaaaggcg
    18241 aaaaagggga caaaggcgac cccggactgc ccggtccaaa aggcgaaaag ggggacaaag
    18301 gggacaccgg agctgccgga ccgatgggaa tgacgggccc agcggggccg atgggaatga
    18361 cgggtgccaa aggggacaaa ggcgatcctg gagcagtcgg accgacgggg ccgatgggac
    18421 ctgcgggcac gacaggtgcg accgggcctg cgggcgcgac gggacctgcg ggcgcgacgg
    18481 gacctgcggg cagcaaaggg gacaaagggg atcgcggcga tcctggaccg caaggcccca
    18541 cgggacctgc gggcacttct gcctatgcgg aagatgtggc ctcgtttgct ggttacacga
    18601 cggggactac gacgggcgcg atcacaaatg gtcggtacgg aatgcatgcg ctgtgcgcgg
    18661 cggcctttcc gggttcgcac ctgtgtcaca gcagtgaata cgcatttaca aactccgcgg
    18721 taaacccacc caccgacgga gcctggatcg atccgtcgtt taccattggc atttccgggg
    18781 tccgccagct ttccggtaca agctgtgata gctggagcca gaccgcatcg ggttactacg
    18841 gtgtctatgt cacgccatca gggggacttg tgacgaccga ctgcgtaaaa cgcaaagcac
    18901 tggcgtgttg taacagtgtg gcgcgcacgc agtttgtcgg cttcaccacc gcaacgacca
    18961 gtggcgccgc cggcagtcgc ttcaaaatgc accagacctg cgcggcggaa tttgcgggtt
    19021 cccacctgtg ccactccatt gagtacgccc gctctcattc cgctgtcgtc ccgcccgcaa
    19081 acggagcgtg gatcgatcca tcgaacggca acctcgctgg aaacgcgcgc gatttggccg
    19141 gcaccagctg tgacaactgg acgcagatgg catcgggtta ttacggagtt tacgtcacac
    19201 cgaacggcag cttggtgtcg accgactgcg tgaaagtgaa gtccttggcc tgctgtcggt
    19261 agcgatttcg ctaaaacaac atcagcagca gcggacgacc aaacagcgca aagcccaagt
    19321 gggcgtgcgg ccacaaagca agtcccgctc caccgacgag aatcagccac attcctgagc
    19381 acagataaag cagtgccggc agccgttttt gcaaaagcgg aggcagctgc agaagcagcc
    19441 gtggtagctg ggtcgcgaca agcagaatcg gtgtggtcag tccgaccaaa agcagcatca
    19501 caagcgcacc gtgcagaggc gatccagtca gcgccgcttg cgacagcgcc cacgctggaa
    19561 tcatgcacgg cagaaagccc atcatcgcgc ccagcgccag ctcgcgcagc ggatgtcgac
    19621 tggcgagcag cggcaaaagc gactgcctgg cgcgcacaag gacagactcc accacttgcg
    19681 caggggacag cgacagcgat ctgccaccac gcagcttgac aagcggcgcg tcgctgggat
    19741 ttggacgcag cgtccgcagt gccattccgc agagcagcac gccaaacaaa agcgccagga
    19801 ccgctccccc ggtttgtgct gcgtgcgaca gacccgctcc caacaatccg gccaagctac
    19861 cgagccacgc ataggtgagc ccacgccccg cttggtacag cagcacgccg ccgattcctt
    19921 gccagcgatt ccgtccacgc atcaccccgg cgatgtcgaa accggacaaa agcggcccgc
    19981 acatgccgac gcagtgaatg ctggtccaca gaacgcccag tcccagcact tgcgcatacg
    20041 ccggaggaag cgtcatatca gtgcgaattt cccgtcgctg ggcttgtggg tccggtcagc
    20101 gcggcatcca tgattcgatc ggtcttggat gcgctgtctg tgaccttgag cttgaccctt
    20161 atgccggcgt gataatcggc gcgcggaatc gaggcaatca ccggcacttc gatgctcgcc
    20221 agcgattcca gttcaacggt cggctgcgga atgatgagcg tcaaaggtgc cgtcgctgcg
    20281 ctttctatct gaagcgtcga cttgactgga tttttgttga tcaggtggac gacgaactgg
    20341 ttgcgcacga tgtcgccatc aatgacaaac gtcatgccgc gcgcgtgaag caggttcgat
    20401 tcaaacgcgg tgtggcgcag gaacagcgtc gtcgatacca caagtcccaa catcccgagc
    20461 agcaggtaca gataaagccg cggacggaca aagcgtcgct tttccccgag aagtccgcgc
    20521 tctgagtcat agcggataag tcctttggga cgcccgagct tgaccatgat ttcgtcgcag
    20581 gcatcaatgc agccggcaca accgacgcac tccatttgca ggccgttgcg gatgtcgatt
    20641 ccggttggac agaccgcaac acagcgatga cagtcgacgc agtcgccgac ccctgggcca
    20701 tgcgctttgc cgcgtggttc gccgcgtttt ttgtcgtacc cgatgacgac ggtatcgggg
    20761 tcttgcatga ccgattgaag ccgaccatac ggacagacaa tcagacagag ctgctcgcgg
    20821 aaccaataga aattccagta aaacaccgcg gtcattccga cgccccacag aaacgtcgaa
    20881 acgtgcgcaa acggtccgtc ttggatcatc cgcagcagct gtttggccga cacaaagtac
    20941 gccaaaaaca gatgcgccag cacatacgcg atgaccacat agcagatgtg cagcgcggct
    21001 ttgcgcagaa ttttttccgc tgtccatgga cctctcgcga gcgcttgccg cttgctcgcc
    21061 gggccttcga taagccgttc aatccgtcgg aacacgcctt ccaaaaagac agtctgcgga
    21121 cacgcccagc cacaccaaac gcgaccaaac agagccgcca tcacaatcag cgaaaagccg
    21181 attccggtca ggataaaaaa cgccagatac aagtcctggg cattgaacgt gcgaccaaac
    21241 aggaaaaacc gtcgcgctgc gatgtccagg tagaccgccg gatgcccacc gatggtgatc
    21301 cacggcagcg agatgtacac cgcgatcagg atgtaaaaca cgagcggtcg cagcttggaa
    21361 aagcgaccgt gtacatcggc aggatagatg tactgtcgcg acccacgctc gtcgagcatg
    21421 tgcagcttat caagagtcgg ttcttcgcgt ttcatcggaa tactctttgg gtccagcgta
    21481 tcccccacac gctgaaccgc caaactggcg ccttccccag cgagcgtgcg gaaggcggag
    21541 gggaactact gggccggggc gggctctcct gattcggtct ttccttgcgg tggcttgcca
    21601 gcgacatgtt tgccgcgcag ggacaggaca aacgccgtcg cttcttgcac tttggtcgcg
    21661 cccagcaccg tcttccaggc aggcattccc ttttccagca cgccggttcc tatcacttga
    21721 taggtgtctt tggctttggc gccatgcaga aagtagttgt cggtcaggtt cggtccgaca
    21781 gtgccttgtc cttcggctcc gtggcaagcg aggcagtttt gtttgaacac cgctgcgccg
    21841 cgctccagca ccgcagcatc tttgctcatc gccaggagct gctcttcggt gactcccttg
    21901 ccggcggctt cctgttccgc catccgcttg tcttccgcct tcttcgcttc atcgagttct
    21961 ttttggtacg ccaccatcgg caaatcgccc gtttgcgacg tgtgatagta gaaccagtag
    22021 ccgaaagaaa acacgatcgt cgcgtaaaac gtcagcagcc accagcgcgg cagcggattg
    22081 tcaaactcgc gaatgccgtc gtagtcgtgg ttggtttcga catcttgtcc gggcgcagca
    22141 ccggcgttgg ccgtgcttcc ttgttcttgc gcgctcatga ctcacactcc tttgcgctgc
    22201 gaacctgccg gcagcaccgg ggcacgcttg acttcgccgt cgtcgccaag cggcaactga
    22261 gcgatcgatt gaaacacgga cgcttgcttt ttgcgaaagg tcgcaaccac caccgcaaaa
    22321 aacgacaaca gaaacgccag catcgccaaa atgggcagga ccaggactcc cttttgactg
    22381 aaaaactcgt ggtacatagg gagtcctcct taccgggtca cttgggcgag cggggccacg
    22441 ccttggctgc gaccgagccg ctgcagatac gcgataagcg ccaccatctg gctgtcccaa
    22501 tcgacggtga ttccctgacc tgcgagatcc ttggcaatcg ctttcgcttg cgcttcaagc
    22561 tctgcattgc cattgccaat ctctgctgcg ctatacggta cgcccagcgt tttcatcgcg
    22621 ttcattttca ccgccgtgtc acccagatcg agcttgctct tggccaggaa tgcatacggc
    22681 ggcatgttcg atccctgcga cgtcgatcgc gggtccatca ggtgctggta gtgccacagg
    22741 ttgccgtact tgccgcccaa gcggtgcagg tccggacccg tccgcttgct gccccactgg
    22801 aatggatgat cgtaaatgaa ctcgcccgcg gtgcttggat ccccgtagcg cagtttttca
    22861 cttgggaacg gacgcaccat ctgcgaatgg caggtgtagc agccttcgcg aacgtacaga
    22921 tcccggccat gcagctccag cggtcggtag ggcttttgca ctgcgcccgt catcggcact
    22981 gcgcggtcga tcaggatcgt cggcaacagc tcggcgacgc caccaatcag gaccgcgatg
    23041 agcaccagcg cagtaaagat gagcgaccgg ccttccagcg tgcggtgcac attcagcctg
    23101 ccgctgtcgt cttttttcat cgcatacgag aaccacatca ccagcacaaa gccagtgaga
    23161 agcgccacgg tcggaatcca gattttcact gaatcaatca ggaagaatgc tgccattccg
    23221 acgagcgcca ccaccgaaag cagcagcgga cgcgaaaaca gaatcgccgt ccatgccact
    23281 tccttgtgtg attcctcttc ttcgaccacc gtgatcgacg tttcgactgc ttcccccgca
    23341 gaaatcgtct tccacaggtt cacaatcatc agcacaaagc ccgtgatgta aagcagtccg
    23401 cctaccatcc gcgtcaggta catcggacga atcgccagca gcgtttcgac gaagcttggg
    23461 tacagaagcg cgccttcggg attgaccgct ttccacataa gaccttgggt cacacccgca
    23521 atccacatcg agatggtgta aagcaggatt cccaccgtcg cggtccagaa gtgcagatcc
    23581 gccagcgacc gcgagtgaag cggacgattc cacagcttgg gaaccagata gtagaacata
    23641 ccggctgcca taaagccgtt ccaacccagc gctccggtgt gcgcgtgacc gacgatccag
    23701 tccgtgtagt gcgcaagtcc ggacacgctc tttatcgaaa gcagcgggcc ttcaaacgtc
    23761 gccattccgt aaaacgtcac gcccgcgata aagaacttga tcaccgggtc ggtgcgcagc
    23821 ttgccccacg cgccccgcag cgtcaaaagt ccgtttacca tgccacccca cgacggtgcc
    23881 cacagcatca ccgaaaacag cattcccatc gactgcgccc agtcgggcag cgcggtgtaa
    23941 agcaggtggt gcggacccgc ccagatgtac atgaagatga gcgcccagaa gtggacgatc
    24001 gagagccgat aggaatagac cggacgttcc gccgctttcg gaaggaagta gtacatgatt
    24061 ccgagaatcg gcgtcgtcaa aaagaacgcg accgcgttgt gtccgtacca ccactgcacc
    24121 agcgcgtcct gcacacctgc gtagatcgaa taggacttca gtcccgaaaa cagcggcagg
    24181 gccaagctgt tgacgatgtg gagcacagcg acggtcaaga tcgtcgagat gtagaaccac
    24241 agcgcaacgt acaggtgttt ttctttccga atcgccagcg tccaaaagaa gttgatcgcg
    24301 aacaccaccc agatgagcgc gatcaggatg tcgatcggcc attccagctc ggcgtattcc
    24361 tttccttgcg taaaaccaaa cggcaaggtg agcgccgcac agacgatgat gagctgccag
    24421 ccatagaagt ggatcgccga cagcaggtcg gaacccatcc gcgacttaca cagccgctgc
    24481 gtcgagtagt agacaccggc aaacatcatg ttgccgacga acgcaaagat cactgcgttg
    24541 gtgtgcagtg gacgcaagcg tccaaagctt aggaatgacg ccaggtttag ctctgggaat
    24601 gcgagctgtg atgcgatgag gaggccgacg atcatgccga tgatgcccca gaatatggag
    24661 gccagcatga accttcggac cgtgacatca tcgtacgtaa tccgtacttc tttcatgacc
    24721 tacgttcctt tgttcgggaa ggggaggggg aaaccttgat ttcctcgtcg gaatccaagg
    24781 gcaataggga caaccgatcg gcgtattcgt gactgcgccg cgtatacaga aatccaaaca
    24841 gaattcctgc ggcgatcaca agcagaaggc tcacaaacag ggtgaggtac agcgcattca
    24901 tgaagacact cccagcaaaa gcggggactc cgtttcactt gatttttcgt cgggggcgtg
    24961 cggtatgttc tgatctgttg cttcgtcttt gtcacgcagc gaacgcaccg tggcaaagat
    25021 cacgaccagc gagctaagcg gcatcgccag cgcacagcgc agcggtgtca taagccccgc
    25081 aaacgacaga gacagaacga tcaaattgta aaaagatgaa agtcccaggt tgcgcaagat
    25141 ggtgcgacgc agcgctttcg ccgtctttag cgcttcactt atcgggccga ttccgacccc
    25201 tgaaaaatag aagtcagcgc gggctggcag cgtcgggcga tcaatcgccg gagtgcccgc
    25261 acaagccgcc gccgaaaacg cgagcgcatc gttgattccg tccccaatca tcagcgtatc
    25321 gccatggccg tcatcgatct gtttgaccat cgcgcacttt tgttcggggg acagaccgcc
    25381 aaatgcgtca caaatgccaa gctgccgtcc gatgcggctg actttgtctt gccgatcccc
    25441 ggacaggatg aatagtgaca gaccttgtgc tttgagcgcc gatacctcgg acgctgcatc
    25501 gcgacagagt gcttccgaaa gggaaaaacg cgcaagcgga acgccatcgc agccaaacac
    25561 cgactgcggc tcgctcgaat cggaatcgga ctgggaatcg gcttgggaat cggcgtctgt
    25621 cggcaacaca aagcgcggcg ttcccagccg ataggtgtga ccgaagcggc caagtgaaag
    25681 acccagtccc gcgtgttctt ccacttgggc tgtcgcatcg ataccgtcct cggtgccaag
    25741 ctgggaaatc gccgacaaaa tcgcccggct gaccggatga ttcgatcgcg ccaccatttg
    25801 aaacagcgcc tgcctttgtt cgctggacag accatgcagc ggcgctttgt tcgcaagtgt
    25861 cagctcgctg agcgtcaccg ttccggtctt gtccagaagc actttgcgca gtccgagcgc
    25921 gcgatcaaaa aacgatggtg tccgcacaaa cagaccacgc cgccgcagcc gcgcttgcgc
    25981 aagttcatag gccagcggcg tcgcgattcc gagcgcacag ggacaggtca cgaccagcac
    26041 cgccaccgct acttccgtcg cccgcagcac tcctgatcga aaccacagcg caaaagcggt
    26101 cgtcgccaaa agaagcacca aagagacata atatcgactg aggtagtccc aaaaatctgc
    26161 gcgctggccg cttgcagcaa cgtctccggg cagcggtttt tcgacgctgg gtgcacagag
    26221 cagatcgcgc agacgtgacg cactgaacgc ttcatcggca gagagtcgga ccgcttcttt
    26281 tccgcggttg tgggcccctg cgggaatggc ttgtttttcg gcgtattgct tgggcgtcga
    26341 ctctcccatg atccagtcga gcgaaaactc tgccgtccca tcgagcagcg tcgcctgcac
    26401 cggcagaagt tcgcccggcg cgcacagcag cacttggtct ttttggatct gcgacgccgg
    26461 caggatttcc agacggcttg tgccatcggt ccccgtccga atcgttcgca caagcagtcc
    26521 agacaccgta tcgtccgcca gcagcctttg ccggttgtga ctcgcgagcc gtcgctgaag
    26581 gagccgcccg agcagcatca gcgtaatgaa aatgcacagc gtatcgaaat aggaagcgcg
    26641 tccctcgccg gcaaagaacg accacacgct gccgacgtag gtcaaaagga tgccgagcgc
    26701 aatcggaaca tccatgtgca agatgccgcg cgaaatcgct tgcacggcgc tgcggaagaa
    26761 caccgttcca ccgattaaca ccgtcgctgt gcccagtgcc agaccgccgt acccaaacag
    26821 cttgtagatg atcggttctt cgcgctgcga cagaccaaaa taggtcgaaa acgcgaaaat
    26881 catgctgttc atcgaaagcg ctgcgcttaa gccaagccgc agcaccaagt catcgacttt
    26941 ggcatcgccg tctttgcggg ccggccccag ccgatagccg agcgcttcga catcatcgac
    27001 aaactgctcg acggtttggc gaccgtgctg cgtgcggcga tacaaaagct cgatccgacc
    27061 gacggcagga ttgaccatga tgcgggcggc accgggcttt ttcaggaaca gcttttccaa
    27121 aagccataca cacgccgcac actccagccc ttggacatcg agccgcagcg ccacaagctc
    27181 ggcatcggcg gccttgctct cggcttggtc cagcagcggc gtaagccaca ggcgctcgcg
    27241 tttttccccc cgactgccca ctgccgacag ccgcgtcgcc ccgcgcagtg cataaaaatt
    27301 gagcaggccg gctgactgca aaagaccatg cgccgccttg cagccaacgc agcagtacgg
    27361 atggtaggcc gcgtcagacg aagcgggtga gttgcagtgg ggacaaacgc taagagcggt
    27421 catggccgct cggctccttg gttgtttcgg tccgcgcagt gtgccgcgcg tctctctcct
    27481 tggggaagca aaggctttgc caagactgct cagccacggg actcggtcag cgcggacgaa
    27541 aactgttccc tatcggaaca caactttgac cctttgtgta cccaaacggt acacgccagc
    27601 cccgcgcaag ctctcccacg aatctgcgct tctgctaaag taccgacttg ccgatgcagg
    27661 ccccttccct cgcacgtcac tttcacctgc tcagcgatgc ggatttgcag gcgctggcac
    27721 ccgtgctgcg tcatctgcgc gagcagcgga cgcgggttct cgacgagttt tataagcgct
    27781 acctgattca ctttggccaa gaagtcagcc ttggattttc cgacttttcg gcgctgtatg
    27841 gtcgcgatct ggatgcgatg gtgcggcacc tggccgatct cgacatggac gactttgccg
    27901 ccgaagtgcg ggccgtcggc tatgaaatga gcgagcgcgg cgtgccgttt gccgaagtga
    27961 tctcgtcgct gcatttgcta gaggaagcgg tcggaacggt gctcgggcct gccgtcgcgc
    28021 ggggcgagct tcagcctcgg gtgtttcaga ttttcgacaa gctcagccac tgccggatca
    28081 ttgtgctcgc cggggcttat ttcaccgggc agtctgccga cgctgagcag cagctgcaca
    28141 atgtctcttc gctgcaagat ggcagcgact tggcgcggca gttttcgtcg ctggtcggtc
    28201 aaagtgaagc catgcgacgg ctgtttgcgc agcttcaagc ggcggcacta agccctggcg
    28261 gagccgccct gctcgtcgga gaaagcggca ccggcaaaga gcttgcggca cgcgcgctgc
    28321 atcaaaaaag ccgtggcgta aagcgtccct tcgtcgccgt aaactgtgct gcgctgccac
    28381 gcgatctgat cgaaagcgag ctgtttggcc atcgtcgcgg cgcgtatacc ggcgcgcaga
    28441 ccgaatacgg cggtctggtg cgcgcagcgt cgacagggac attgtttctc gacgaaatca
    28501 ccgaaatggc agttgaagtc caagccaagc tgctgcgcgt gctggaagaa aaagcagtgc
    28561 gcccagtggg agcacctggc gaaattgccg tcgatgttcg ctttatcgca tcgaccaatc
    28621 gcgatcccca aaaagccgtg gccgcagggc tcctgcggga ggatctgtac taccgcatca
    28681 gcgcctttcg ggtcgacttg ccgccgctgc gtgagcgacg gggcgacatc ccgcttctgg
    28741 tccgccactt tgccgagctt ttgcacaagc ggggacttcg cgcgatcgaa caattcgacg
    28801 cggcggcgct gcgtgctctc cagacgtata cctggccggg caacgtgcgc gagctgcgaa
    28861 acgcgatcga ttttgcgctg accgtcggaa gtggtcccgc gcttctgcgc gacgatctgc
    28921 ccccgcatgt gttgcgtggc gcggcgccgc cgcttccggg cgatgcccca gccagcgggg
    28981 attccccgtc gagcgcggag gccagtgtac ccgcgtcaga caaagattcc gccggcgctg
    29041 tccccaccat ggaagaagcc gagcgcgagc tgatccagcg ggcgctgtca gcgacgggcg
    29101 gaaacaagct gcaagcggcg cggatgctcg ggatttcgcg ccatcgtctg tatgacagct
    29161 tgcggcggtt tcggatcgaa gacgccgaag cctaagccgc gaactgttcc caaaaggaac
    29221 actgtggcct gcttcctgtg tggccaagat gtggccggac ttttgcatga tctcgccact
    29281 tgatggggct ttaccgcaaa cccgcagaca caaggcgcag cgctccccgc gcgatccgcg
    29341 tcgctgcgtc ggattcggcc aaagattcgg cgtcggctga ttcgcagggc cgcagcgcaa
    29401 gccgagctcc gacgctttac gccaacatga tcgagcagca tcacgagatc gatcagctgc
    29461 tcagccggct tgtgcaggcc gtggcgcaca tcaatgggcc tcccgaacgg gcgcaggcac
    29521 ggcgactttt gacacggctg cgcaagacgc tgtacgccca catctcactc gaagacggaa
    29581 ttctgtttcg cgagtttgaa tcccagagcg gactgctttt ggaaggcccg acggcgacgc
    29641 tccgtcgtga acatcaggtg ctcaaagcgc ggatcgaaga gctgtcggta tcgatcgaca
    29701 acggtgaaaa aacgccgacg ctcttgcggt tgctcgaagc gttttgtgcg ctgtacgccg
    29761 atcacggtcg tcgtgaagag gtgatgtacg gaatttgtga acggcttttg tcccccgagc
    29821 tcctcgcgaa agcgcagacg ctgctgcgcc gcaagccatc ccgcagcgga gactagcgga
    29881 tcgtcgtcgg cgtcttgcgc gtgcttgtgg aagagtggcg gatcgactgc atcgatcgta
    29941 tagaaccctc tatgccgtgt ccggggcgtg gcggcacttc tgccggctgc gccgtcgcgt
    30001 tcatgcagtc accgcacgtt acgaggtcta ca
//
LOCUS       DAORGN010000038        29835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_578_length_29835_cov_5.807085, whole genome shotgun sequence.
ACCESSION   DAORGN010000038 DAORGN010000000
VERSION     DAORGN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 29835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29835
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_578_length_29835_cov_5.807085"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2459)
                     /locus_tag="PKL17_06515"
     CDS             complement(237..2459)
                     /locus_tag="PKL17_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK44415.1"
                     /translation="MSLFPRRRRLFMRAAGSGLAFGLLLASACSPARLGVDEEDGLRD
                     GGDSTSTDMSTGPGKPCGADSPCPMGWLCHMGQCVPDRGTCCSDNDCQNDTRCSVQVG
                     DGGSCGVCLDWKDGESDAMCKGNGFSATEFKAPIDHCQWPAKGAPAPVASDVVMTPLV
                     IDLDGDAKPEIVFAPQVSAGPSRLVAMSGKDCTVRYDVDANIQGFSQIAAADLDGDKR
                     PEIIALLATGAPGSGHSIGVFEGTTGKLITSSTDAFALSGGVSFDCSGPAVADLDGDG
                     APEIVVGGMALRFNKAKKQLEKMFNSPVVGATWGTLTLVNDMDGDGKPDIISGNRIFD
                     GQTGVDKTPSSMASLAQGGYPAIGDFNKDGSPDIVFVQSASNDQKVAVIDVKADKFLM
                     PMTSIPGGWGGPPTVADFDGDGTPDFGTAGPRSYYVFSLDCLKTPKPAKCRGTLPGVL
                     WNTVTKDVSSGGTASSVFDFNGDGRAEVVYRDECWLRVYNGPDGKTVFARPITSGTAL
                     EMPIVADVDNDGHADLIVPSDSIQGNGYCADQNPERDTGMAHTGVTQGVFVLKDPMNR
                     WMPSRPLWNQHTYHITNVNDDGTIPAKETPNWSTYNNYRQNIQGTLVKTVPLPDATSK
                     GSPSPDQVDCSKQWKLYAQVCNRGSDLIATMIPGTFYKKDPRIDPASAICVAYTTAPI
                     PPGKCEVVSCAWDKPVQGSVDLWFRANDDGKGARPQPQCKNGNDLSFMPGAHCTQGPG
                     "
     gene            2830..3468
                     /locus_tag="PKL17_06520"
     CDS             2830..3468
                     /locus_tag="PKL17_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44416.1"
                     /translation="MKKRSTSGELQDLIKQLTTIRKEHVRALDEIENTFRMFGLDRLI
                     KDGAGKKRAGKKAAVKAAPSAPSKKKAGKAKKAAAPAATATKAPKAAKKAKATKAPKA
                     ATKAPKATKAPKATKAPKAAKSAGKRRGSFEITGDELILRFVKDKGNATTEQIRKHWE
                     ASSRGGKSDNNLTNLVKSGKLVRSKDPSGPGSLYSLPASAAASTAPTTTPTA"
     gene            complement(3568..4449)
                     /locus_tag="PKL17_06525"
     CDS             complement(3568..4449)
                     /locus_tag="PKL17_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44417.1"
                     /translation="MTLLLALTMLAGMDVGNGSRYGYEGDLFDNVGTFACRRTLTVRY
                     GQKGWQKMRNHGVAHRTLPCGTRLGICNPRTGRCTAAYVVDRGPWGALDRNGEWHVRT
                     GPLRPGEHYRGVLDLLPGVYSAIDLQGIEKVYYWPLPDPQQSQASRENRLPPLRSHGP
                     SPYAFYLAPSHRSTPFPPLRDLNAEVLLAGVTVKKESRHGELPPLRGFELPAPLPSPQ
                     LLAAVVPPQPPPPSLSPTLSGMSAVLPVNAVGRSFPPLRDASAPQPVETVQSRFGMGR
                     SAKAQPVVPVRLLPANS"
     gene            complement(5028..5981)
                     /locus_tag="PKL17_06530"
     CDS             complement(5028..5981)
                     /locus_tag="PKL17_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK44418.1"
                     /translation="MKFFVTGCAGFIGSHLCHRLLGLGHEVLGLDCFDETLYPAALHR
                     AQLAPLLSHPAFRFVEGDILSAGLLAELTKEASAVIHLAALAGVRPSLFQAARYMRVN
                     VEGTQNVLDACRQNGIRRIVFASSSSVYGAHSAVPFREDDTAMRPASPYAASKRAGEL
                     ICSTHADLYGAQIAALRFFTVYGPHQRPEMAIASFAARILRGDPVVLHGSGTSARDYT
                     YIDDIIDGIIAAIDRTADSQLGFRAYNLGGERTTKLADLVTRLESSLGRSAQIEWRPD
                     QPGDVPITWAQLDRARHELGYAPKVDIAQGIARYCDWLRSR"
     gene            complement(6009..6515)
                     /locus_tag="PKL17_06535"
     CDS             complement(6009..6515)
                     /locus_tag="PKL17_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44419.1"
                     /translation="MHRRLVRIGLGFSLLFAASANAQIPPLRSAYPPSQTPPPGSPLI
                     GRDLRKVRIGALIWAAGYLPALVAPLVLWPRVESGEGPSALANYSLLVPVVGPLVSAI
                     AAPAQTDDGNGRAVMSSWSVPWLLTSGLLQATGLAILISGARPHLLLDDSLVLFPTTN
                     GVMAAGRF"
     gene            6827..7624
                     /locus_tag="PKL17_06540"
     CDS             6827..7624
                     /locus_tag="PKL17_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK44420.1"
                     /translation="MKRALPFVFLLGLAAGCGIPKEQWEAKLKENADLQMKVTDLEKH
                     KQKLESEIADLKKEKADLQRTIDNLKDSMGKLGKDKADLLERMAKLSATLEDLERAKA
                     AADKRSKAFRDLLAKFKAMMDAGKLQVEVRNGLMLVKLPDNILFDPGKTELKKEGQEA
                     IGQVTRILNSIEGRKFQVAGHTDNVPTGKNSRFKSNWELSSQRALTVLDLMVKEGMDA
                     KRLSAAGYADVLPVATNDTEAGRKKNRRIEIVLQPNIEDLPGIDEKQ"
     gene            7802..8677
                     /locus_tag="PKL17_06545"
     CDS             7802..8677
                     /locus_tag="PKL17_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44421.1"
                     /translation="MTSMKLKLSLAAAFALVMPGVASAQGAPDPNGQPPPGYYAPPPP
                     AGYGQPYQPPPPQPYQPDPAYQPQPYQPQPYQPQPYQPQPYQPAPMYQPQPQVTRSFL
                     YRPQFRPQFGIAARFGGYGNYGSFGSYSTGGGGVDFLLRAHPRLTFELGIQYQRISQE
                     DVYLNDFQYERYDVPMTLGMRVHLGNPLWIVSPYLVGAGGLTYSRLYMLDALTYESHW
                     FGEVQGGIGFEWRLGQHFAFNLDLRGQGRFRKVGELGTPTSNEGTLTDVYGNTVPSMG
                     GSGGFILNMGIGGFF"
     gene            8741..10153
                     /locus_tag="PKL17_06550"
     CDS             8741..10153
                     /locus_tag="PKL17_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44422.1"
                     /translation="MAGARVKKLLDQGISLHQAGRISEAEALYRKVLSLSPSDPDAQH
                     MLGVAAIQRGDFERAIPFLHRARNSYPRMPELHNHLGMAQRGLGKLTEAQGSFRTALL
                     INPRFVDAHVNLADVLTQQGLIGDALSQIQHALRIDSSHVESYNMQGNILHRQRRYAE
                     AVASYQQGLARNPASGLLWQNMGNTLREMKDFAAAATAYQEAMRRQPKDARICLLLGA
                     TLEKLGQPEAAEAAYREALKRKPSWSEADFFLSALRSRTGDEQAPATAPQEFVTQLFD
                     AYASNFDQHLIEKLKYRAPELLSDALTPHWPTEPGVVIDLGCGTGLLGPLIRPHASQL
                     HGVDLSPNMIQAAAQRRVYDHLELGEMVACLAGRPAQFDAVVATDVLIYVGDLAPLFA
                     KAHAALRPGGLFAFTVEENTRDSGYWLQPSRRYAHAADYLRKLAAERGFVEKSLSTDV
                     IRQQSGQAITGHIVVFRRNP"
     gene            complement(10163..11416)
                     /locus_tag="PKL17_06555"
     CDS             complement(10163..11416)
                     /locus_tag="PKL17_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44423.1"
                     /translation="MKRHPLCLPGVWLLATSGLVTGCDNQLSLEHRLTSLRMVLRSPM
                     ESELGSPTNPVTPSTLKFDIEALDEKGKTFPFDGNLSAYLIAGGNRLSLVDPCSSATP
                     TGDPTVLKTIGMRSGKVTGEQVPLSLPPVFGRIAINLEEPQSQALGSTPAIYFPNPTI
                     ARLMKPLDVTAGNASYCSPYLSRQVTVDTTATAAGKLIVSSVFQNGLAVSDTSSPDYG
                     SMYVFTFSQPSAQIQIGTILDSMSGAIAKFNGMTQLANPVLIPTSSFRPDLVPAPVEL
                     DAAHRPTGGPTSANNQWLTKSIAAPVRVTGIVCDVVADSNRASNWEKYNTVVINQVDS
                     DPKSSTGCGGVNLSYDSGLGTRFNVQLPGKGFGGFDPVQYAGQEATFIGMLQNGASKS
                     GKTLFWTAVVRRTADVCLKPKAQCP"
     gene            complement(11429..13690)
                     /locus_tag="PKL17_06560"
     CDS             complement(11429..13690)
                     /locus_tag="PKL17_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNK44424.1"
                     /translation="MLQKRHRRQTPWIFVLSLLLGLSACTYSREPEDLRGQDVRLRLI
                     HTSDVHSRLLPYQYAPNLPDRNLGLNVLPGVCTESRTCSSDFSRTCSTDSDCSAFPTA
                     SVGGLARMATLVKRERAKSPRTLHLDSGDMFQGAPVFNLFNGEVEVRTMGMLGVQGMA
                     LGNHEFDKGAVLLGIQLMNNGPNFPILAANYEWFDPDDQRAPKLGRFIQPVSVYNVRG
                     IKVGVIGLGNVSSLNSSVEGDNSLGLRARDSKQAVSDLVRVLRPQVDLMVVVSHMGPD
                     DDVAVSREVSDNDSEVEQNDPNSAAVDLNGIDVVLGGHLHTVYYPPVDISHYDKTGSY
                     QGHTIIVNSGAFAKFIGSLDMVVHVGDPASADPLLRRSFVKSYTFDILPVGERPWQPG
                     DPTDPCDPSIRCDPPHPSNPKVTCPYRGAMPAPRPNVICTPPDGDMSRVLEPYEIKMK
                     QLLNLGQVYAVVPCPTGSSSCPKVLRNDAGGGDSQLGNLVATSMRLRRRVEADFALTN
                     SLGIRADFESGPLTLEQLYNVFPFDNTITTQFLSGDEVLQMLDFVADRSSERGCKTQV
                     QVSGIYFTLNCQNQRCPCRTKPELCDCAKTADCQTYRAQCPTSLSCSDRLSSTVAQKS
                     KWVGPFADQIVLGDDCRNIDGTANKDKCKPLDCFASYKVAVNDYISVGGSGFVVLKRN
                     TTRFNTGISLRDALADFIRTLGTDDRYRCKDATKYSNIKGRGPTGDYDYSSVTCLLPE
                     VFAHDGRIQPLVR"
     gene            13958..14488
                     /locus_tag="PKL17_06565"
     CDS             13958..14488
                     /locus_tag="PKL17_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNK44425.1"
                     /translation="MQTKDATPPAEVDSDGSQPRQDKLLRIGQLAELCNKTTRALHLY
                     EELGLLRPVIRSRGGFRLYSPTAIERVQWISRLQDADVSLGEISQLLRDLEEQRVGTE
                     AMTKLRTLLDAKLRETREQIEKLRRLEQDLQAGLSYLDGCKVCSSETPTSECGGCRIH
                     GHDGPQPLMVAGVHVS"
     gene            14505..15719
                     /locus_tag="PKL17_06570"
     CDS             14505..15719
                     /locus_tag="PKL17_06570"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNK44426.1"
                     /translation="MKLPIFMDYHSTTPVDPRVLQAMLPYFTTHFGNAASRNHAYGWT
                     AEKAVDDAREQIGQLIGASGKEIVFTSGATESNNLALKGAAHFYKDRGNHIITTQIEH
                     KSVLDTCKRLELEGFEVTYLPVESDGRLRPEAVRAAMTDRTILVSVMLANNEIGTIQP
                     LKEIGALVKEKGALFHTDAVQGIGKIPFDVSDVQADLVSLTAHKLYGPKGVGALYVRR
                     KPRVRLEAQMDGGGHERGLRSGTLNVPGIVGFAKACELAQKEMATESARIASLRDRLE
                     SSLMKALPHVFRNGSAEHRLPGNANLSFAYVEGEGLLMALKDVAVSSGSACTSASLEP
                     SYVLRALGIGEELAHTSIRFGLGRFTTDEEVDFVIQLTTDKVRRLREMSPLWEMVQEG
                     VDLKTIEWTAAH"
     gene            15752..16159
                     /gene="iscU"
                     /locus_tag="PKL17_06575"
     CDS             15752..16159
                     /gene="iscU"
                     /locus_tag="PKL17_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNK44427.1"
                     /translation="MAYSSKVIDHYENPRNVGSFDKNDPDVGTGLVGAPACGDVMKLQ
                     LKIDDEGVIQDARFKTFGCGSAIASSSLVTEWVKGMSIEDAEKIQNSAIADELNLPPV
                     KIHCSVLAEDAIKAAIADYRRKKGPGLETEKAS"
     gene            16162..16503
                     /locus_tag="PKL17_06580"
     CDS             16162..16503
                     /locus_tag="PKL17_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNK44428.1"
                     /translation="MGIQATTKAIAEVKRLQEKRAAEGKLAQGLRIGIRGGGCTGFSY
                     LFEWADEPPRSTDKVFEFGEDVRIFVDPKSLVYLNGTELDFVTSMMGYGFKFQNPNTK
                     GSCGCGESVQF"
     gene            16484..17050
                     /gene="hscB"
                     /locus_tag="PKL17_06585"
     CDS             16484..17050
                     /gene="hscB"
                     /locus_tag="PKL17_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNK44429.1"
                     /translation="MRASSSKSYFALLDLPESYSLDAAELERRYREKSRHWHPDRFSR
                     APASERAAVLQRATELNEAYRTLRSDGKRAEYLLKLYGVDLADESKKPALAPGFLAEI
                     LELREALADARVEGNSAQLADIKKTVQAELAKLASRLHDGFARLLAADPAALSEVTEL
                     CYVQRYYQRFADDIAEFEETAAGHLAVL"
     gene            17106..19061
                     /gene="hscA"
                     /locus_tag="PKL17_06590"
     CDS             17106..19061
                     /gene="hscA"
                     /locus_tag="PKL17_06590"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNK44430.1"
                     /translation="MSLFQIAEPGESKAKDACKGRVIGIDLGTTNSLVAVVQDGEPTC
                     LRDEHGSALLPSVVRYRKNAAPLVGKEAKLTAFEAPHDTIASVKRFMGRGPADIAKDG
                     TAQRFGQYLFADPSLFADGVIRLVVAGPSGMRPVTPMEVSAEILRVLRARAEQQLGSE
                     LEGAVITVPAYFDDAQRQATKDAGRLAGLSVLRLLNEPTAAALAYGLDKKREGKFAVY
                     DLGGGTFDVSILDLCDGIFTVMATCGDTALGGDDMDSALAELIASKHPQGQTLRSQIA
                     ADPVRSRHLLDAARTMKHQLTDADTATTNWEGAALSITRSEFDAAIAPILQRTAGPCR
                     RALKDAELTASELTGVILVGGSTRVPAVRRFVAELFGQTPLCDLDPEQVVALGAAVQA
                     DILASERQDALLIDVTPLSLGLALMGGIVEKIIPRNSRVPTGAKQVFTTYADGQTGFD
                     LHVVQGERETASECRSLARFKLTGIPKMAAGMARLEVMFLIDENGLLQVGARELTSGR
                     AAHIAVKPSYGLSDEQIEKMLVDSFEHAEGDLRKRALLEQRVEAERILQALETALSQD
                     GHLLSEEEQQPILEARTALQAARNGVDHRLIADCITALDVASKEFAQRRMNAGLSRAV
                     AGKTVGSVEQKVHYDENDLPKTVPLAE"
     gene            19066..19419
                     /locus_tag="PKL17_06595"
     CDS             19066..19419
                     /locus_tag="PKL17_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNK44431.1"
                     /translation="MPIVTFLNDPGADGKPKSIDVAPGTSILEAAKRTSAQVGYACGG
                     VCACSTCHVYVKNGLSSLSAQKDREEDILDKAFDVRANSRLGCQAKLGSGNVEVEISR
                     ESRRAFFDEHPDARG"
     gene            complement(19450..21597)
                     /locus_tag="PKL17_06600"
     CDS             complement(19450..21597)
                     /locus_tag="PKL17_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK44432.1"
                     /translation="MPRHLVLRTLLPALMFSFSVADGAVAQEESSLATTVTADKPESA
                     ASAQTVRDRDLQLRPIQRVADLLRVVPGLLTVQHAGGGKANQYLLRGFDADHGTDVAF
                     SIDGVPINLVSHGHGQGFSDMNFVIAELIERVEISKGPYFVEQGDFATAGAVNLVTRK
                     KFASNQVSFSVDTLAGLRALAVASPQLQSLGEKLHPLVVAEVSRTSGPFLHPEDYHKY
                     NVFGKVSYDLGPQTTISLSASSYGGSWNASGQLPSRAVDAGLVDFYGSLDPSEGGEST
                     RHNLYLALHHRDGLRSDVSVLVFGSLYRFKLYSNFTLYSADPIRGDEIEQGDERLLGG
                     LKAHYRRRFVWHFVRADTTLGVSVRHDSIANTLYHAQARQRIDALTDHDVRETSLGVF
                     LKEEIAFSSFVRLVAGLRADGFFFDVTDRRELLPSDGTRPVDEAQKTSGTRSAQLLSP
                     KLSLIVSPHRVIDLFANVGYGFHSNDARGVVRGKAPVTPLTPALGYEIGARTRLSDRL
                     DLALALWGLDLRGELVWSGDEGTTEASGATRRLGLEFETRLRIWDFLHFDFDFTANDA
                     RFRETTERGNAVPLAPRVTLSSGLSLQTKFGLRGSLRFTGITQRPATEDEFLIAQGAY
                     LFDAFLGYRFRFVEVSVSVENLLNQRYKQAQFAITSRLPSEPSQSAPPPPSACPSSTR
                     AKSGSDGNFLGCEDVSFTPGYPINARFTVSLFY"
     gene            complement(21489..22148)
                     /locus_tag="PKL17_06605"
     CDS             complement(21489..22148)
                     /locus_tag="PKL17_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44433.1"
                     /translation="MKLGRSQRFCTTPLRRYRLSQLCLFLCAIAVHGLPWLHLAWHRP
                     DHVHVGGGQRFVHPTEKTQSHDGSDEAGHVHSHAGHSHSRRVLGWFGSLQGRLPLVEI
                     VADDGSGHDDLHLPLGAAHGLSFVAPTRFVPWWDVGVHRQAAGEPGISSLRLRIVSAR
                     ARAPPGWVCTDRTDSQPISRSRQRCQGILFCARFCLRSCFHFPSLMARLPRKNPRLQR
                     R"
     gene            complement(22279..24006)
                     /locus_tag="PKL17_06610"
     CDS             complement(22279..24006)
                     /locus_tag="PKL17_06610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44434.1"
                     /translation="MAKRRECPSCRSVYQGDDKLFCPKDGTRLVEQVARPSEDPWIGR
                     VIAGRFVLERRLGKGGMGVVYLANHNVLRRPFAVKLLRREFVSNERALARFFREARVA
                     SSVDHANIVSIYDYGQTDKGEPYLVMEYVEGTLLYQLVVSSPGKCLHPLQAVDISLQV
                     ARALEHAHQRGVVHRDIKPENILITTVGGQTDFVKVLDFGVARVIGQPPLTRVGEEII
                     GTPEFIAPEMMSASGELSPSVDLYALGIMLHDTIVGEPPWRGDIKEILNGHMNVVPPR
                     LSDRRKDALIPRELDELAAQLLEKNPNRRPTATETVEILTRIRALLPARSVQLRRGDQ
                     PKTKSDAEPTLSGKETQLLPRPSAVAPQLRAETVILPRNEPNAAARQAEIEAIDSEIV
                     GLLARLSTLIEQVGQKLWVDKWPEKPQMLRELLVGNTLAQEKRQQRINRLHDQVQQQQ
                     TRLQQHRSELRQQILALSERLQLETQLPESEQKRIKRLIEEKERLLAQVLFSAPTSPE
                     PELYLLRRELRELTEQARSGWPQLARLLIDDPKAEKFPERGTVELVLEQLSAAQAMQV
                     LLQRQNVAI"
     gene            24167..24427
                     /locus_tag="PKL17_06615"
     CDS             24167..24427
                     /locus_tag="PKL17_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44435.1"
                     /translation="MNPKHTILSSLSNHLAGAISALAKLSRRVGAKVVDYVLVAGQHP
                     SPVRSPEIAAFTALAAERAKRRAAQSQAAPAAALLASATLTM"
     gene            complement(24447..24896)
                     /locus_tag="PKL17_06620"
     CDS             complement(24447..24896)
                     /locus_tag="PKL17_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44436.1"
                     /translation="MSHVPDEVRFQDTTAGISSSGRDVLAKLLSAVPALCFIHCVGTV
                     LLALALPAAASLWTAGEWLEGPLWLLSIGVVGTLLYRRRQLRGVTGALLSLSLLLGLV
                     GFGLDSELARRISLLGLVALQLLSWQRRQKLALVPVCPCAHHQRLAA"
     gene            25032..26435
                     /locus_tag="PKL17_06625"
     CDS             25032..26435
                     /locus_tag="PKL17_06625"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HNK44437.1"
                     /translation="MQDRQPISPQAVYFARHALLPSGMCKNVRIELSDDGSFSSVTPQ
                     ASPDGATELPGVVLPGMVNLHSHAFQRAMAGLTERGGPLTDSFWTWREVMYHFLRSLG
                     PDDVEAIAAQLYVELLRGGFTGVVEFHYLHRDPQGELYENPAELALRISHAAEQTGIG
                     LTLLASLYQQGGFGAEPLSPGQRRFYLSDDQFGQVITTLMAHRRPIDRIGIAPHSLRA
                     VTPQALEQATQIIESIDPRAPIHLHISEQPAEVAACLSWSQKTPIDWLLDHVAVDGRY
                     CLIHATHATEAEISRLARHAVTIGLCPSTEANLGDGIFAAAALISGGGRIGIGTDSHV
                     GTSALDELRLLEYSQRLRHGRRNVLAKQEGVSTGESLWLAAAGGGAQASAQPTGVLAA
                     GSRADLVVLSDDRPALYGRHGSQILDAAMFGPDRDAVSDVMVQGRWVVRSRRHFAEES
                     IAARFRSTVERLAARGP"
     gene            26493..27380
                     /locus_tag="PKL17_06630"
     CDS             26493..27380
                     /locus_tag="PKL17_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNK44438.1"
                     /translation="MQRPFRTFLGVDLGGGKGKTTAMVRLRLTGQGDKPLVVEDCGSG
                     KPYFDDRLLTLVEQHKDDAVLAVDAPLTLPVCVRCTLASCPSLTHCSVDTVRWFRDRT
                     THEASSGKPRYTPYTQRATEVILHEVHQILPRETLGQGMGPLTARALYLRQALRKSYV
                     LNQNLIEVYPKATLTQLFPDPAPPQPASAIRYRDAQGRPVSIEGKPLHPGSTVARTYK
                     RGHGPDVRKDVLSSLPELSFATGQWREAVIKNDHIFDALICAYTGYLWARDGWQLPKD
                     PVFADDGYIWTPPRRNNSI"
     gene            27516..29027
                     /locus_tag="PKL17_06635"
     CDS             27516..29027
                     /locus_tag="PKL17_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK44439.1"
                     /translation="MKSWYRLLFLGTTAVAVVAASCNHDNPGTSDLGGADFAVGSDMA
                     GSGSDDGGGDGGAVSTDMAMPLALTAASPPTGPTTGNIDVTISGTGFLSGAFVTIDGQ
                     SATVKSVTSTAIVVTLPARPGTKGPVAVVVTNPGGKTVMSSTIFAYYFGTLSFDTAVK
                     TTVGGTPTNIAITEIENNAKPDIVVTDNSSIGQIVTLSGNGNGTFLTPAKFAAGISTY
                     GLKVLDMDADGFNDVVVTNASSAPSGVGIHLNSKTGTFPSRTPVVTGDIPFSVDAKDI
                     NGDGYIDLVVANGGGSSNNLAFLLGKVGGTYAAPVNLTAGTVPRSVVFVDVNKDGKTD
                     IVAANESTANVSVFIGDGTGNFGNKKDYQAGSGPYSVAATDFNGDGNVDLIVCNSRDA
                     TIALLTGVGDGSFNAPKTTSLGTPNTSLPYQIAIGDINGDGRPDVVSAIYADQQIGIT
                     YNANGLFGTTTKLAASGFPRGVAVGDLNGDGTTDIVSANSTDGSISIILNKSK"
     gene            29117..>29835
                     /locus_tag="PKL17_06640"
     CDS             29117..>29835
                     /locus_tag="PKL17_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNK44440.1"
                     /translation="MRQFSSDSADENVITIDSDSAPRILFYGEDFLCEDLPVGTKVIY
                     PRRPISGLPNPRAAIRYALHHPEDMEPLHALLFPGMKVTIAVDDISLPLPPMQKPDIR
                     ETICSIVLEMLADHGVDDIHIIVATSLHRRMTEREIRYMLGDKIVDAYWPDNLYNHDA
                     CDPERMVTIGKTRHNEVVETNRRAIESDLVIYVNINLVPMDGGHKSVGVGLCGYESLK
                     AHHTPKTIVDSQSYMDPKNSAL"
BASE COUNT         6307 a         9284 c         8880 g         5364 t
ORIGIN      
        1 cacgccatgc gcaccagccg tcacgccatg cgcaccagcc gtcacgccgt cctcacactc
       61 cgtcacgccg tcctcacact ccatcacgcc gtcctcaccc tccatcacgc catgcgcacc
      121 agccgtcacg ccgtcctcac actccgtcac cacgtaacgg cgggtcgaca gcgcagtttt
      181 tggtgaaggg tggggaagca gacaggggga agcgggaata cgggcggcga cgtgggctag
      241 ccggggcctt gggtgcagtg ggcgccgggc ataaacgaca ggtcattgcc gtttttgcac
      301 tgcggctgtg ggcgggctcc cttgccgtcg tcgttggcgc ggaaccacag atcgaccgat
      361 ccttgcaccg gcttgtccca cgcgcagctt acgacttcgc atttgccggg cggaatcggc
      421 gccgtggtgt aagcgacgca gatcgccgac gccgggtcga tgcgcggatc tttcttgtag
      481 aacgtgcccg gaatcatcgt cgcgatgagg tccgatccgc ggttgcagac ctgtgcatac
      541 agcttccact gcttgctaca gtcgacctga tccggcgacg gcgagccttt gctggtcgca
      601 tcgggcagcg gcaccgtctt gaccagcgtg ccctggatgt tctgccggta gttgttgtac
      661 gtggaccagt tgggcgtctc tttggccggt atcgtgccgt cgtcgttcac attggtgatg
      721 tgataggtgt gctggttcca cagcgggcgt gacggcatcc agcggttcat cggatctttg
      781 aggacaaaca ccccttgcgt caccccggtg tgcgccatgc cggtgtcgcg ctcaggattt
      841 tggtcggcgc agtagccgtt gccttggatg gaatcggacg gaacgatgag gtcggcgtgt
      901 ccgtcgttgt ccacgtcggc gacaatcggc atctccagcg cggtgccgga cgtaatcggc
      961 ctggcgaaca cggtcttgcc atcgggtccg ttgtagacgc gcagccagca ctcatcgcgg
     1021 tagacaactt cggcgcggcc atcgccgttg aagtcaaaga ccgatgacgc agtgccgccc
     1081 gatgacacgt ctttggtcac ggtgttccac agcacaccgg gcaacgtgcc gcggcacttg
     1141 gcgggcttgg gagtcttgag gcaatcgagc gaaaacacgt aataggaacg tggtcccgcg
     1201 gtgccaaagt ccggcgtgcc atcgccatcg aagtccgcga cggtgggcgg gccaccccag
     1261 ccaccaggaa tcgatgtcat cggcatcagg aacttgtcgg ctttcacatc gatcaccgcg
     1321 actttttgat cgttgctcgc ggactgcaca aacacgatgt ccggactgcc gtctttgttg
     1381 aaatccccaa tcgccggata gccgccctgc gcaagcgacg ccatcgagct tggcgtcttg
     1441 tccaccccag tctggccgtc gaagatgcgg ttgccagaga tgatgtccgg cttgccgtca
     1501 ccatccatgt catttaccag cgtgagcgtg ccccacgttg cgcccaccac cgggctattg
     1561 aacatctttt cgagctgctt tttggctttg ttaaagcgca gcgccatgcc gccgaccacg
     1621 atctctggtg cgccatcccc atcgagatcc gcaacggcgg gaccggaaca gtcaaacgac
     1681 acgccgcccg acagcgcaaa ggcgtccgta ctgctcgtaa tcagcttgcc ggtggttcct
     1741 tcaaacacac caatcgaatg tccagagccc ggcgcccccg tcgcaagcag cgcgatgatt
     1801 tcgggccgct tgtcgccatc caaatcggca gccgcaatct gcgaaaagcc ctggatgttg
     1861 gcatcgacat cgtaccggac ggtgcagtct ttgccgctca tcgcgacgag gcggcttggg
     1921 ccagcagaca cctggggcgc aaacacaatt tcgggcttcg catcgccatc caaatcgatc
     1981 accagcggcg tcatcaccac atcactggcg accggagcgg gcgcgccttt ggccggccac
     2041 tgacagtgat cgatcggcgc tttgaactcg gtggcggaaa agccgttgcc tttgcacatg
     2101 gcgtcggact ctccgtcttt ccaatcgaga cagacaccgc agctaccgcc atcaccgacc
     2161 tgcacgctgc agcgggtgtc gttttggcag tcgttgtccg aacagcaagt gccgcgatcg
     2221 ggaacgcact gtcccatgtg acacagccaa cccatcggac agggggaatc ggcgccgcat
     2281 ggcttccccg gacccgtcga catgtccgtc gacgtggaat cgccgccatc gcgcaggccg
     2341 tcttcttcat caactccgag ccgggcggga ctgcatgcgg aagcgagcaa aaggccaaac
     2401 gcaagacccg accccgcagc acgcatgaaa agccgacggc gacgtggaaa cagcgacatg
     2461 gtgactcctt gacggaaacg gctcaatttc tgccgaaaat cgccgcgact ataacaagcg
     2521 ggctcagcca gatcgagcgt ttcacttctc gatttcaaaa gtcgcccagc ggaacagacc
     2581 ccctaggttg cgcaagggaa gcttctgcaa gggcccccta agcggcctcc caaaccacta
     2641 cagagacact cctaagcgca ctccttttcg cggatttccc gtcggatttt gcgaacagag
     2701 agcccccttc ttcattgttt tgcgaaatgc gcgcgattca ctcttgtacg caccggcggg
     2761 ctcatgtacc tttcgcccgc ttttcgcaaa cactacttcc gcgacgtaaa gacgtaaaaa
     2821 ggaatcacca tgaagaagcg tagtacctct ggcgaacttc aggatctcat caagcagctc
     2881 accaccatcc gcaaggagca tgtgcgggcg ctcgacgaga ttgagaacac cttccgcatg
     2941 ttcggactgg atcggctcat caaagacggc gctggcaaaa agcgcgctgg caagaaagcc
     3001 gcagtgaagg ctgctccttc ggccccgagc aagaagaaag cgggcaaagc caagaaggct
     3061 gcggccccag ccgcgacggc gaccaaggcc cccaaggccg cgaaaaaggc caaggcgacc
     3121 aaagccccca aggccgcgac caaagccccc aaggcgacca aagctcccaa ggcgaccaaa
     3181 gcccccaaag cagccaagtc ggctggcaag cgccgtggct cgttcgagat cacgggtgac
     3241 gagctgatcc tccgcttcgt caaggacaag ggcaacgcca ccaccgagca gatccgcaag
     3301 cactgggaag cctccagccg cggcggcaag tcggacaaca acctcaccaa cctggtgaag
     3361 agcggcaagc tcgtgcgcag caaagatcca agcggccctg gttcgctcta cagcctgccg
     3421 gcttctgctg ctgcttcgac cgcgccgacc accaccccga cggcctaacc agccgccccc
     3481 atctttccgc ccgatttttc catcccaaac aacatcgccc aggctgtgtc catgacacag
     3541 cagggtcgtc ttgtgctgcg ctgacgacta gctgttggcg ggcaggaggc gcaccggcac
     3601 caccggctgc gctttggcag agcgacccat tccgaagcgc gactgcacgg tttcaaccgg
     3661 ctgtggcgcc gacgcatcgc gcagcggtgg gaatgaccgt cccacagcgt tcaccggcag
     3721 caccgcgctc attcccgaaa gagtcggtga cagactcggc ggtggcggct gcggcggaac
     3781 caccgcggcg agcagctgtg gcgacggcaa aggcgcaggc agctcgaagc cgcgcagtgg
     3841 tggcagctcg ccatggcgcg actctttttt gaccgtcacc ccagccagca gcacttccgc
     3901 attgagatcg cgcagcggcg gaaacggcgt gctgcgatga ctcggcgcca aatagaaagc
     3961 atacgggctt ggtccgtggc tgcgcagcgg tggcagacgg ttttctcggc ttgcctgcga
     4021 ctgctgcgga tcgggcagcg gccagtaata gactttttcg attccttgca ggtcgatcgc
     4081 cgaatagaca cccggcagca gatcgagcac cccgcggtag tgttcaccag gacgcagcgg
     4141 cccggtccgc acatgccatt cgccattgcg gtcgagcgcg ccccacggtc cacgatcgac
     4201 gacatacgcc gccgtgcaac gcccggtccg cgggttacaa atcccaagcc gagttccaca
     4261 aggcaacgtg cgatgggcca caccgtggtt gcgcatcttc tgccaacctt tttggccata
     4321 gcgcaccgtc agggtccgac gacaagcaaa cgtccccacg ttgtcgaaca gatccccttc
     4381 atagccgtag cgtgagccgt tccccacatc catcccggca agcatcgtca gagcaagaag
     4441 caacgtcact ggcgccctcc cccgcgcttc gctacccagt gaacgtcgcc gatccgctct
     4501 gcgcagaaag acggtctaac actgcgatct gtagcgtaag tgcgccaatt acaacacatt
     4561 tgcaatgccc agattacgaa acaccgcgta gaaggatatc aaacagagct gcgagtccga
     4621 tccaacaatt tttatcccac agcgaaattg gtgcgataac gtaggaatgt tgcgcaggtt
     4681 ctctgcctga attgacgcgg tgcgtaaaat ccgacccaaa gaccagattt tccgtctgaa
     4741 acactgcaaa gaccccccaa gctgacgcca acatgtggct caacaaccga aactggtcgg
     4801 gccacagatg ccaactattc aacaaacaaa tatcatcaat aatgaaaaca aaataaatca
     4861 ataaccatca acacaaaaat cacacaagat caatcaaaga tgcctatttg aaaaaaagga
     4921 cgcaacattc tttccgtcga aacttcgtcg atggagagat tgccggataa acgtggggcg
     4981 tacagcgggc gaggaggaga gaaaaaacgg cggcggaaac ggcggggtca gcggctgcgc
     5041 agccagtcac aatagcgggc gatgccttgg gcgatatcga ccttgggtgc atagcccagc
     5101 tcgtggcgcg cgcgatcgag ctgcgcccag gtgatgggca cgtcgccggg ctggtcgggt
     5161 cgccactcga tctgcgccga acgtccgagc gacgattcca agcgcgtcac aaggtcagcc
     5221 agcttggtcg tccgctcgcc acccaggttg taggcccgga agccgagctg gctgtccgcg
     5281 gtacgatcaa tcgccgcgat gatgccgtcg atgatgtcgt ctatgtaggt gtagtcgcgg
     5341 gcgcttgtgc cgctgccgtg cagaacgacg ggatcgccgc gcaaaatccg cgccgcgaag
     5401 ctggcaatcg ccatttcggg acgctgatgc ggaccataga cggtgaaaaa gcgcagcgcg
     5461 gcgatttgtg caccataaag atcggcatgg gtcgagcaga taagctcacc agcgcgcttg
     5521 ctggcggcgt agggggacgc gggccgcatc gcggtgtcgt cttcgcgaaa ggggacggcg
     5581 ctgtgtgcac cgtagaccga gctggacgaa gcaaagacga tgcggcggat tccgttttgg
     5641 cgacacgcat caagcacatt ctgggtgcct tcgacattga cgcgcatgta gcgagcggct
     5701 tggaaaagcg acggacgaac tcccgccagc gccgccaaat gaatcaccgc cgatgcttct
     5761 tttgtcagct ccgcgagcag ccccgccgac aagatgtcac cttcgacaaa acggaacgcc
     5821 ggatgggaca gaagcggtgc cagctgcgcg cggtggagcg ccgccggata cagcgtttcg
     5881 tcaaagcagt cgagcccgag aacttcgtgc cccaaaccaa gcagccgatg gcacagatga
     5941 ctgccaataa acccagcaca gcccgtgaca aaaaacttca tccccgtccc tccctgctct
     6001 gcagcagatc aaaagcggcc cgccgccata acgccatttg tcgtcgggaa aagcacaagt
     6061 gaatcatcga gcagaaggtg cggacgcgcc ccggaaatca atatcgccag gccggtcgct
     6121 tgcaaaagcc cgctggtcaa aagccacggc acgctccacg acgacatcac cgcgcgcccg
     6181 ttgccatcgt cggtctgcgc cggggcagca atcgccgaaa cgagcggacc gaccaccggc
     6241 accagcagcg aatagttggc gagcgcgctc ggaccttcgc cgctttcgac ccgcggccac
     6301 agcaccagcg gtgcaaccag cgcgggcaga tatccggcgg cccaaatcaa cgcgccgatc
     6361 cgaactttgc gcaggtcacg tccgataagc ggcgatccgg gcggcggggt ttgcgatggt
     6421 ggatacgcag agcgcagcgg cggaatctgg gcgtttgccg aagctgcaaa cagcagggaa
     6481 aacccaagcc cgatccgcac aagtcgacga tgcatcgctc gcaagagtag cgcgccgcgc
     6541 ccaaagccga aaagtgtgca gactgcttca cgaagcatca aggcattgct cgacggtacg
     6601 gatgacccga tcgcttgcat gtggaccagc ggaccgtcgt attccggtcg ggccgggccg
     6661 aattgcacgg cgaatcatcc atggccgcgt tttaccaaag cgagtcgcct acccccaaac
     6721 attcaaacga cgccacccgc gagtgtgtga cgactgtcgc cgcgaggcgg tttcttccct
     6781 gcccagatgc ggttaggatg aaatgagcca atacggagaa gtttccatga aaagagcatt
     6841 gccctttgtc tttctgcttg gcctcgcggc tggctgcggc atccccaagg aacagtggga
     6901 agcaaagctg aaggaaaacg ccgaccttca gatgaaggtc accgacctgg aaaagcacaa
     6961 gcagaagctg gagtcggaga ttgccgatct gaagaaggaa aaagccgatc ttcagcgcac
     7021 catcgacaac ctcaaggaca gcatgggcaa gcttggcaag gacaaggccg acctgctaga
     7081 gcggatggcc aagctgagcg cgacgctgga agacctcgag cgcgccaagg ctgccgccga
     7141 caagcgcagc aaagcattcc gcgacctgct cgccaagttc aaggcgatga tggacgctgg
     7201 caagctgcag gtcgaagtgc gcaacggtct gatgctggtg aagctgcccg acaacatcct
     7261 gtttgatccg ggcaagaccg aactgaaaaa ggaaggccag gaagccatcg ggcaggtcac
     7321 gcgcatcctc aacagcatcg agggtcgcaa gttccaggtc gccggccaca ccgacaacgt
     7381 cccaacgggc aaaaactctc ggtttaagtc gaactgggag ctgtcgtctc agcgagcgct
     7441 caccgtgctg gatctgatgg tcaaggaagg catggacgcc aagcggctgt cggctgcggg
     7501 ttacgctgat gtgttgccag tggcgacaaa cgacaccgag gcgggacgga agaaaaaccg
     7561 ccgcatcgaa atcgtgctcc agccgaacat cgaagacctg cccggcatcg acgaaaagca
     7621 atagcactgc gcacgctcgt tcacagcgac gactcagtct gtgaaccatc cgctacagtt
     7681 tcaggcccgc cgccgcaaaa tccccctggc acaactactg ctacgataga ccggcaacac
     7741 ctcgtctagg aacacgcgcc cggcctggtt tgtcagtcgg cgcgtcgaaa ggaacttcgc
     7801 tatgacttcg atgaagctga aactgtcgct tgctgccgcc tttgccctgg tgatgcccgg
     7861 cgttgcttct gcgcagggag cgcccgatcc gaacggtcag ccgccgccag gctactatgc
     7921 gccgccgccg ccggctggtt atggtcagcc ctatcagcca ccaccgcccc agccgtatca
     7981 gcccgatccg gcctatcagc cgcagcctta tcagccgcag ccttatcagc cgcagcctta
     8041 tcagccgcag ccttatcagc cggcaccgat gtatcagcca cagccacagg tgacgcggag
     8101 ctttctgtat cgtccgcagt ttcgcccaca gtttggcatc gccgcacgct ttggcggcta
     8161 cggcaactac ggttcctttg gcagctacag caccggcggt ggcggtgtcg atttcctgct
     8221 gcgcgcccat ccgcggctga cgttcgagct tggcattcag taccagcgca tctcgcagga
     8281 agatgtctat ctaaacgact tccagtacga gcgctacgat gtgccgatga cgctgggaat
     8341 gcgcgtccac ctcggcaatc cgctgtggat tgtgtctccg tatctggtcg gcgcaggagg
     8401 cctcacgtat tcccgactgt atatgctcga cgcgctgacc tacgaatcgc actggtttgg
     8461 cgaagtccaa ggcggcattg gcttcgagtg gcggctcggc cagcacttcg cgttcaacct
     8521 cgacctgcgt gggcaaggac gcttccgcaa agtcggcgag ctcggaaccc cgacgtcgaa
     8581 cgaaggaaca ctgaccgacg tgtatggcaa caccgtcccg tcgatgggtg gaagcggcgg
     8641 tttcattctc aacatgggaa tcggcggctt cttctaaatc caaacccgca tagattcccg
     8701 taagcaaatt cactggatta tcctaggaag ctaggaatcc atggctggcg cacgggtcaa
     8761 aaagctcctc gatcaaggta tttcgctgca ccaagcaggc cgcatcagcg aggccgaagc
     8821 gctgtaccgc aaggtgcttt cgctgtcgcc aagtgatccc gatgcacagc acatgctcgg
     8881 tgtcgcggcc attcagcgcg gcgattttga acgggcgatt ccgtttctcc accgtgcacg
     8941 caacagctat ccacgcatgc ccgagctgca caaccacttg gggatggcac agcgcggact
     9001 gggcaagctg accgaagcgc agggatcgtt ccggaccgcg cttttgataa atccgcggtt
     9061 tgtggatgcg cacgtcaact tggccgatgt cttgacgcag caagggctga ttggcgacgc
     9121 actttcacaa attcagcacg cgctgcggat cgattcaagt cacgtcgaaa gctacaacat
     9181 gcagggcaac atcctgcacc ggcagaggcg gtacgcagaa gcggtcgcaa gctaccagca
     9241 gggtcttgcg cggaatccag cgtcgggact gctctggcag aacatgggca ataccctgcg
     9301 cgaaatgaaa gacttcgcag cggcagcgac ggcgtatcag gaagcgatgc ggcggcagcc
     9361 caaagacgcg cgaatctgcc ttttgcttgg cgcgacgctg gaaaagctgg gtcagcccga
     9421 agccgccgaa gccgcatatc gcgaagcgct gaaacgaaaa cccagctggt ccgaggccga
     9481 tttctttctt tctgcgctgc gcagccgcac gggcgacgaa caagcgccag ccaccgctcc
     9541 acaagagttt gtgacccagc tcttcgacgc ctacgcgagc aattttgacc agcatttgat
     9601 cgaaaagctc aaataccgtg cgccggagct gctcagcgac gccctcacac cgcattggcc
     9661 gaccgaaccc ggcgtggtca tcgatctcgg ctgtggaacc ggacttttgg gaccgctcat
     9721 ccggccccat gcgtcgcagc tgcatggcgt cgatctgtcg ccgaacatga ttcaagcagc
     9781 agcgcaaagg cgagtctacg atcacctgga gcttggcgag atggtcgcct gcttggcagg
     9841 aagaccggcc cagtttgacg ccgtggtggc cacggacgtg ctgatttatg tgggggactt
     9901 ggcaccgctg tttgcgaaag cccatgccgc gctgcgaccg ggtggtctgt ttgcattcac
     9961 cgtcgaggaa aatacccgcg acagtggata ttggctacag ccttcgcgtc gctatgccca
    10021 cgctgcggat tatttgcgca aactggccgc tgaacgtggt ttcgtcgaaa aaagcctgtc
    10081 gaccgatgtc attcgtcagc agagcggaca ggcgatcacc ggccacatcg tggtcttccg
    10141 tcgcaatccg tagcggcaca cgttacggac actgggcttt gggttttaga cacacgtcgg
    10201 cggtacgccg caccactgcc gtccaaaaca gcgtcttgcc gctcttgctg gcaccgtttt
    10261 gcagcatgcc gatgaacgtc gcttcttgtc ccgcgtactg aaccggatcg aagccgccga
    10321 agcctttgcc gggaagctgg acgttaaagc gcgtgccgag accgctgtcg taagacaagt
    10381 tgacgccgcc acagcccgtg ctgctcttcg gatcgctgtc gacttggttg atgacgacgg
    10441 tgttgtattt ttcccagttg ctcgcgcggt tcgaatcagc gacgacatcg cagacgatgc
    10501 cagtgacccg gacgggtgcc gcgatcgact tggtaagcca ctgattgttc gccgaagtcg
    10561 gtccacccgt cggacgatgc gccgcatcca gttctaccgg ggccggcacc agatcaggcc
    10621 ggaacgacga cgtcgggatc agcaccggat tggcgagctg ggtcatgccg ttgaacttgg
    10681 caatcgcgcc cgacatgctg tcgagaatcg taccgatctg gatctgcgcc gacggctgcg
    10741 aaaacgtaaa cacgtacatg ctgccgtagt ccggcgacga cgtatcactc accgccagcc
    10801 cgttttgaaa caccgacgag acgatcagct tgcccgctgc cgtcgcggtc gtgtcgaccg
    10861 tcacctgccg ggacagatac gggctacagt acgacgcatt tcccgccgtc acatcgagcg
    10921 gcttcatcag ccgcgcaatc gtcggattgg gaaagtagat cgcaggcgtc gagccgagcg
    10981 cttggctttg cggctcctcg aggttgatgg cgatgcgtcc aaagaccggc ggcaggctga
    11041 gcggcacttg ctcgcctgtg accttgccgc tgcgcatgcc gatggtctta agcaccgtcg
    11101 gatctcccgt tggtgtcgcg ctagagcacg gatcgaccag cgacaagcga ttgccgcccg
    11161 cgatcagata cgccgagaga tttccatcaa acggaaacgt cttgcctttt tcgtcgagcg
    11221 cttcaatgtc aaacttcagc gtcgacggag tcactggatt ggtcggtgat cccagctcac
    11281 tttccatcgg actgcgcagc accatccgca ggctggtcag acgatgttcg agcgaaagct
    11341 gattgtcgca gcccgtcaca aggccgcttg tcgccagcaa ccacacgcca ggaaggcaca
    11401 aagggtgtcg cttcatcgaa aactcccgtc agcgaaccaa aggttgaatg cgcccgtcgt
    11461 gagcgaagac ctcggggagc agacaggtga cgcttgagta gtcgtagtct cccgtcgggc
    11521 ctcggccttt gatgttactg tacttggtcg cgtctttgca gcgatagcgg tcgtcggtgc
    11581 ccagcgtgcg aatgaaatcg gccagcgcgt cacgcaagct gatgccggtg ttaaaccgcg
    11641 tggtgttgcg cttgagcacg acaaagccgg aaccaccgac ggaaatgtag tcgttgaccg
    11701 cgactttgta gctggcaaaa cagtcgagcg gcttgcactt gtctttgttt gccgtgccgt
    11761 cgatgttgcg gcagtcgtca cccagaacga tctgatcggc gaacggaccg acccacttcg
    11821 acttctgggc caccgtcgag ctgagccgat ccgaacacga cagcgacgtg ggacactgcg
    11881 cgcgataggt ctggcagtcg gcagtctttg cgcagtcgca cagctccggt ttggtccggc
    11941 aggggcagcg ctggttttgg cagttcaagg tgaagtaaat gcccgacacc tgaacctggg
    12001 ttttgcagcc gcgctcgctg gagcggtccg cgacgaaatc gagcatctga agcacttcgt
    12061 cgccggacaa aaactgcgtc gtgatggtgt tatcaaacgg gaacacgttg tagagctgct
    12121 cgagcgtgag cggccccgat tcaaagtcgg cgcgaatgcc cagcgagttg gtcagcgcaa
    12181 agtccgcctc gacccgacga cgcagccgca tcgacgttgc gaccaagttg ccaagctgcg
    12241 agtcaccacc gccggcatcg ttgcgcagca ctttggggca gctcgaagaa cccgtcggac
    12301 acggcaccac ggcgtagacc tggcccagat tcagaagctg cttcatcttg atttcatacg
    12361 gttccagcac ccgcgacatg tcaccgtcgg gcggcgtaca aatgacattc ggacgtggcg
    12421 ccggcatcgc cccacgatac gggcaggtca ctttgggatt cgacggatgc ggcggatcgc
    12481 agcgaatcga cggatcacac ggatcggttg gatcgcctgg ctgccacgga cgctctccga
    12541 cggggaggat gtcgaacgtg tagctcttga cgaaactgcg acgcaaaagc ggatcagcgg
    12601 aagctggatc accgacgtga accaccatgt cgagcgagcc gatgaacttc gcaaaggcac
    12661 cgctgttgac gatgatggtg tggccttggt agctgccggt cttgtcgtag tgcgaaatgt
    12721 cgacgggcgg atagtacacc gtgtgcaggt gcccgccgag cacgacgtca attccgttga
    12781 ggtcgacggc ggcgctgttc ggatcgtttt gttcgacttc gctgtcgttg tcggacactt
    12841 cgcgtgacac cgcgacgtca tcgtcggggc ccatgtggct tacgacgacc atcagatcga
    12901 cttgcggtcg cagcacgcgc accagatcgg acaccgcttg tttggaatcg cgcgcccgca
    12961 gacccagcga gttgtcacct tcgaccgagc tattgagcga cgagacgttg ccaagaccaa
    13021 ttacgccgac cttgatgccg cgcacgttgt agaccgacac cggctggata aaacgaccga
    13081 gtttgggcgc gcgctggtca tcgggatcaa accactcgta gttggccgcc aggatgggga
    13141 agttgggccc gttgttcatg agctggatgc ccaaaagcac cgcgcctttg tcgaactcgt
    13201 ggttgccgag cgccatgccc tgcacgccga gcattcccat cgtccgcact tcgacttcgc
    13261 cgttgaacag attgaacacc ggcgcgccct ggaacatgtc gccggaatcc aggtgcagcg
    13321 tccgcggcga cttggcgcgc tcgcgtttca ccagcgtcgc catccgtgcc agtccaccga
    13381 ccgatgccgt cgggaacgcc gaacagtccg aatccgtcga acaggtgcgc gaaaagtccg
    13441 aactacaggt ccgcgactcg gtacagacgc ccggcagcac attgagcccg aggttgcgat
    13501 cgggcaagtt cggtgcgtac tgatacggca aaagccgcga gtgcacgtcc gaggtatgaa
    13561 tcagccgcag gcgcacgtct tgaccgcgca aatcctcggg ctcacgagaa tacgtgcaag
    13621 ccgacagacc caaaagcaat gacagcacaa aaatccaagg ggtttgtcga cggtggcgct
    13681 tttgcaacat caggctcctc atccgcagtg agcggtactg tagcaaaaac cccactcccc
    13741 gtcgaaaaac ctccggtgac cgcccttctt tctgaattta tataagtttg acaataagac
    13801 cagccggatc gccgcgacgt gatgtcggat gcggtcctac cgtcctggtt ttccgccaaa
    13861 cttgaactct tacgtaagcg atagattttg cgcttgaccg gcccgaagtg gcatctgata
    13921 ctacgcgaca aatctcacgc acacgtaagg gttagagttg cagaccaagg acgccacacc
    13981 gccagcggaa gttgacagcg acggcagcca gccacgccag gacaagctcc tgcggattgg
    14041 ccagcttgcc gagctgtgca acaaaaccac gcgggcgctg catctttatg aagagcttgg
    14101 cttgctccgt ccggtgattc gttcgcgcgg cggcttccgg ctctattcac cgacggcgat
    14161 tgagcgagtg cagtggatct cgcggctgca agacgccgat gtgtctctcg gggaaatctc
    14221 gcagcttttg cgcgatctcg aagaacagcg cgtcggcacg gaagcgatga ccaagctgcg
    14281 cacgctgctc gacgccaagc tgcgcgaaac ccgcgagcaa atcgaaaagc tcaggcggct
    14341 agagcaagac ctccaggccg ggctcagcta tctcgacggc tgcaaagtct gtagttccga
    14401 aaccccgacg agcgagtgtg gtggctgccg cattcacggc cacgacggac cccagccgct
    14461 gatggtggcg ggcgttcacg ttagttaaag gacgaaccat ggcgatgaag ctcccgattt
    14521 ttatggatta ccactcgacg acgccggtcg atccgcgcgt tctgcaggcg atgctgccgt
    14581 actttacgac gcactttggc aacgccgcga gccgcaacca cgcatacggt tggaccgccg
    14641 aaaaagccgt cgacgacgcc cgcgaacaga tcggccagct catcggcgca tcgggcaaag
    14701 aaatcgtctt tacttcgggc gcaaccgagt caaacaacct ggcgctcaag ggcgcggcgc
    14761 acttctacaa ggaccgcggc aaccacatca tcacgacgca gattgagcac aaatcggtgc
    14821 ttgatacctg caagcggctc gagctggaag gcttcgaggt gacgtatctg ccggttgagt
    14881 ccgacggtcg actgcggccc gaagcagtgc gcgcggcgat gacggatcgc acgattttgg
    14941 tgtcggtgat gctcgccaac aacgaaatcg ggacgattca accgctcaag gaaattggcg
    15001 cgctcgtcaa agaaaaaggc gcgctgtttc acaccgacgc ggtgcagggc atcggcaaaa
    15061 ttccgttcga tgtcagcgac gtgcaggcag acttggtgtc gctgacggcg cacaaactgt
    15121 atgggcccaa aggcgtcggt gcgctgtacg tccgtcgcaa accgcgggtc cgcttggaag
    15181 cgcagatgga cggtggcgga cacgaacgcg gtctgcgctc gggcacgctg aatgtgcctg
    15241 gcatcgtcgg ttttgccaaa gcgtgtgagc ttgcgcaaaa agaaatggcg acggaatcgg
    15301 cgcggattgc atccctgcgc gacaggctgg aaagcagcct gatgaaagcg ctgccgcatg
    15361 tcttccgcaa cggatcggcg gaacaccgac tgcccggcaa cgccaaccta agctttgcct
    15421 acgtcgaagg tgagggcctg ctgatggcgc tcaaagacgt ggcggtgtca tcgggttcgg
    15481 cgtgcacgtc ggcgtctttg gaaccgtcgt atgtgctgcg cgcgctcggc atcggcgaag
    15541 agctggcgca tacctcgatt cgatttggac tcggacgctt taccaccgac gaagaagtcg
    15601 attttgtgat tcagcttacg accgacaaag tgcgtcgcct gcgcgagatg tccccgctgt
    15661 gggaaatggt gcaggaaggc gtcgatctaa agaccatcga atggaccgcg gcgcactagt
    15721 cgccgggtga aacagcaaga gacaaacgac catggcttac agcagcaaag tcatcgacca
    15781 ctacgagaac ccgcgcaacg tcggcagctt cgacaaaaac gatcccgatg tcgggaccgg
    15841 gcttgtcggt gcaccagcat gcggcgacgt gatgaagctt cagctcaaga tcgacgacga
    15901 aggcgtgatt caagatgcgc gctttaagac cttcggctgc ggatcggcga ttgccagttc
    15961 gtcgctcgtc accgagtggg tcaagggcat gtcgatcgaa gacgcggaaa aaatccaaaa
    16021 ttcggcgatt gccgacgagc taaacctgcc tccggtcaag atccactgct cggttctcgc
    16081 cgaagacgca atcaaggcgg cgattgctga ctaccgtcgc aagaagggac cgggtttaga
    16141 gacggagaaa gcgagctaac gatgggcatt caagcgacca ccaaagccat cgctgaagtg
    16201 aagcggctac aggaaaagcg cgccgccgag ggcaagctag cgcaaggtct gcgcatcggc
    16261 attcgtggcg gcggctgcac cggcttttcg tatctgttcg agtgggccga cgagccaccg
    16321 cgcagcaccg acaaagtgtt cgagtttggc gaagacgtgc gcatttttgt cgaccccaag
    16381 agcctggtct acctaaacgg caccgagctg gactttgtca ccagcatgat ggggtacggc
    16441 ttcaagttcc aaaaccccaa caccaaggga agctgtggct gcggtgagag cgtccagttc
    16501 taaatcgtat tttgcgctgc tggatctgcc cgagagctat tcgcttgacg cggcggagct
    16561 ggaacggcgc tatcgcgaaa agtcgcggca ctggcatcct gaccgcttca gtcgggcacc
    16621 cgccagtgaa cgcgccgctg tgctccaacg ggccaccgag ctaaacgaag cctatcgaac
    16681 cttacgcagc gacggaaaac gcgcggaata tttgctcaag ctttacggcg tcgacctggc
    16741 cgatgaaagc aagaagccgg cgcttgctcc gggctttctt gccgaaatcc tcgagctgcg
    16801 tgaagcgctc gccgatgccc gtgtcgaagg caacagtgcc cagcttgccg acatcaaaaa
    16861 gacggtgcag gccgagcttg ccaagcttgc cagccgactg cacgacggct ttgctcggct
    16921 tttggccgca gatccggcgg cgttatcgga agtgaccgag ctgtgctacg tgcagcgcta
    16981 ttaccagcgc ttcgccgacg acatcgccga gtttgaagaa accgccgcag gtcacttggc
    17041 cgtgctataa gccgcgcact ttgccttccg caaaccaccc acccgtttcc ctgtagagag
    17101 atggaatgag cctgtttcaa attgccgagc ctggcgaatc caaagccaag gatgcctgca
    17161 aaggtcgcgt gatcggaatc gacctgggca ccaccaactc gctcgtcgct gtggtgcaag
    17221 atggggagcc cacctgtctg cgtgacgagc atggctcggc gcttttgccg tcggtggtac
    17281 gttaccgaaa aaacgccgcg ccgctcgtcg gcaaagaagc caagctgact gcgtttgaag
    17341 cgccgcacga cacgatcgcg tcggtcaagc ggttcatggg acgcggtccg gcggacattg
    17401 ccaaagatgg aaccgcgcag cgttttggac agtatctgtt tgccgacccg agcttgtttg
    17461 cagacggtgt gatccgattg gtcgtcgcag gtccatcagg gatgcgcccg gtgacaccga
    17521 tggaggtcag cgccgaaatt ctgcgcgtgc tgcgagcgcg ggcggaacag cagctcggca
    17581 gcgagctgga aggcgcggtg atcaccgtac cggcctattt cgacgacgcg cagcggcaag
    17641 cgaccaaaga tgcggggcgg ctggcggggc tttcggtgct gcggctgctg aatgaaccga
    17701 cggcggcggc gctggcttac gggctcgaca aaaagcggga gggcaagttc gcggtctacg
    17761 acctcggcgg aggcacgttt gatgtctcga tcctcgacct gtgcgacggc attttcaccg
    17821 tgatggcaac gtgcggtgac acggcgctcg gtggcgatga catggattcc gccctggccg
    17881 agcttatcgc atcaaaacac ccgcaaggtc agacgctgcg cagtcagatt gccgccgatc
    17941 cggttcgctc gcgacatctg ctcgacgcgg cgcggacgat gaaacaccag ttgacggatg
    18001 ccgatacagc gacgacaaac tgggaaggcg cagcgctttc catcacgcgc agtgagtttg
    18061 atgcagcgat tgcacccatt ctgcagcgca ctgccggtcc ctgtcgtcgc gcactcaaag
    18121 acgccgagct tacagcgagc gagctaaccg gggtgattct cgtcggcggt tcgacccgcg
    18181 tgccagccgt ccgccgcttc gttgctgagc tgtttggcca aacgccgctg tgtgatctcg
    18241 atcccgaaca agtggtggcg ctcggggcgg cggtccaggc cgacattttg gccagcgagc
    18301 ggcaagacgc gctgcttatc gacgttacgc cgctgtcgct ggggctagcg ctgatgggtg
    18361 gaatcgtcga aaagatcatc ccgcgcaaca gccgagtccc gacgggagcg aagcaggttt
    18421 tcaccacgta tgccgatggg cagaccggct ttgatcttca cgtcgtgcag ggcgaacgcg
    18481 aaacggcgag cgagtgtcgc agcctggcgc gcttcaaact gaccgggatt ccgaagatgg
    18541 ccgccgggat ggcgcggctc gaagtgatgt tcctgatcga cgaaaacggg ctgctgcaag
    18601 tcggcgcgcg cgaactgacg tcgggaaggg cggcacacat tgcggtcaag ccgtcgtatg
    18661 ggctcagcga cgagcagatt gaaaaaatgc tcgtcgatag ctttgagcat gccgaagggg
    18721 atctgcgcaa gcgggcgctg ctcgaacagc gcgtcgaggc agagcgaatc ctgcaagcgc
    18781 tggagaccgc gctttcacaa gacggtcacc tgctcagcga ggaagaacag cagccgattc
    18841 tggaagcccg gacggcgctt caggcggccc gaaatggcgt cgatcatcgc ctgattgccg
    18901 actgcatcac cgcgctggat gtcgccagca aagagtttgc gcagcggcgg atgaacgccg
    18961 ggctcagtcg cgcagtggcc ggaaagaccg tcggcagcgt cgagcaaaaa gtgcattacg
    19021 acgaaaacga tctgcccaag actgtccctc tcgctgagta agaacatgcc catcgtcact
    19081 tttttgaacg atcctggcgc cgacggaaag cccaagagca tcgacgtagc gccggggacc
    19141 agcatcctcg aagcggccaa gcggacttca gcccaagtcg gctacgcctg cggcggcgtc
    19201 tgcgcctgct cgacctgtca cgtctacgtc aaaaatggtc tttcgtcgct gtctgcgcaa
    19261 aaagatcgcg aagaggacat cctcgacaaa gcgttcgatg tccgcgcaaa ctcgcggctg
    19321 ggctgccagg ccaagctggg cagcggcaac gtcgaagtcg agatttcccg cgaaagccgc
    19381 cgcgcatttt tcgacgaaca tcccgacgcg cgcggctagt ttcgtggcgt tccgtcgcgc
    19441 aacgccgtcc taataaaaca gggacacggt gaagcgcgcg tttatcggat agccgggcgt
    19501 aaagctgaca tcctcacagc cgaggaagtt gccgtcgctg ccgctcttgg ctcgcgtgct
    19561 gcttggacaa gctgagggtg gcggtggggc gctttgggac ggctcgctgg gcagccgcga
    19621 agtgatggca aactgcgcct gcttgtagcg ctgattgagc aagttttcga cgctgacgct
    19681 cacttcgaca aagcgaaagc gataccccaa aaacgcatcg aacagatagg cgccttgggc
    19741 gatcagaaac tcgtcttccg tcgctgggcg ctgggtgatg ccggtaaagc gcagcgatcc
    19801 gcgcagacca aacttcgtct gcaacgaaag cccgctcgac agcgtcacgc gcggagccag
    19861 cgggacggcg ttgccacgtt ccgtggtctc gcgaaagcgc gcgtcgttgg cagtgaagtc
    19921 gaaatcgaag tgcagaaaat cccagatgcg cagccgcgtc tcgaactcca gtccaagccg
    19981 gcgcgtcgct ccgctggctt cggttgttcc ttcgtcgcca gaccagacca gctcaccgcg
    20041 aagatcaagc ccccacaacg ccagcgccag atcgagccgg tccgaaagcc gcgtccgcgc
    20101 gccgatttca tacccgagcg ccggggtcag cggcgtcacc ggagccttgc cacggaccac
    20161 accgcgcgca tcgttcgaat gaaaaccgta gccgacgttg gcaaacaggt cgatgacccg
    20221 atgcggcgac acaatgagcg acagcttcgg tgacaaaagc tgcgcgctgc gggtgcccga
    20281 tgtcttttgc gcttcatcga caggccgcgt cccatcggag ggcaacagct cgcgacgatc
    20341 cgtcacatca aagaaaaaac catcggcccg cagaccagcg accaaacgga caaacgacga
    20401 aaacgcgatc tcctctttca gaaacacccc gaggctggtt tcgcgcacat catggtcggt
    20461 cagggcatcg attcgctgcc gtgcctgcgc gtgatacagc gtgttggcaa tcgaatcgtg
    20521 acgaacactg acccccaagg tggtgtccgc acggacaaaa tgccaaacga agcgccttcg
    20581 atagtgcgcc ttgagtccgc ccagcagccg ctcgtcaccc tgttcgatct cgtcgccgcg
    20641 aatcgggtct gccgaataca acgtgaagtt ggaataaagc ttgaaccggt agagcgaccc
    20701 aaagacaagg acgctcacgt cgctgcgcag cccgtctcga tggtgcagcg ccagatacag
    20761 gttgtgacgg gtcgattcgc cgccttcgct cgggtcaagc gaaccgtaga aatcgaccag
    20821 accggcatcg acggcccgcg acggaagctg tccagaagca ttccacgaac cgccatagct
    20881 cgacgcggaa agtgaaatcg tcgtctgcgg cccaaggtcg tagctgactt tgccgaagac
    20941 gttgtacttg tgatagtcct cgggatgtaa aaaggggccg cttgtgcgcg aaacctcggc
    21001 gacgaccagg ggatgcagct tttcgcccag cgattgtagc tgtggactgg cgacggccaa
    21061 cgcgcgcaat cccgccagtg tgtcgacgga aaagctgacc tgattgctgg cgaacttttt
    21121 gcgcgtgacc agattgaccg caccagcggt ggcaaagtcg ccctgctcga caaaatacgg
    21181 gcctttgctt atctcgacgc gctcgatcag ctcggcgatg acaaagttca tgtcggaaaa
    21241 accctgtccg tggccgtgcg agaccaggtt gatgggcact ccgtcgatag aaaatgccac
    21301 atcggttccg tggtcggcgt cgaagccgcg cagcagatac tgattggctt tgccgccgcc
    21361 cgcgtgctgc acggtcaaaa gtcccggtac aacccgcaac agatcggcga cacgctggat
    21421 cggtcgtagc tgcaaatcgc gatcgcgcac cgtctgagca gacgcagcgc tttcgggctt
    21481 atcagccgtc accgtcgttg caagcgagga ttcttcctgg gcaaccgcgc catcagcgac
    21541 ggaaaatgaa aacatgagcg caggcaaaag cgtgcgcaaa acaagatgcc ttggcatcgt
    21601 tgtcggctcc ttgaaatggg ttgggaatcg gttctgtcgg tacagaccca tcccggagga
    21661 gcccgcgccc gcgccgacac gatgcgcaaa cgcagcgacg aaatccctgg ttcgccggca
    21721 gcctggcgat ggacccccac gtcccaccac ggcacaaacc gcgtcggtgc cacaaacgaa
    21781 agcccatgcg ccgcaccgag cggaaggtgc aagtcgtcgt gtccactgcc gtcgtcagcg
    21841 acgatctcga caagcggcag ccgtccctgc aggctgccga accaaccaag cacccgccgc
    21901 gaatgcgagt gcccggcgtg ggaatgaacg tgcccggctt cgtccgatcc gtcgtgggac
    21961 tgcgtttttt ccgtcggatg aacaaaacgc tggccgcctc cgacgtgaac gtgatcaggc
    22021 cgatgccaag caaggtgcag ccacggcaag ccgtgcaccg cgatggcgca cagaaaaaga
    22081 cacagctgcg acaggcgata ccgacggagc ggcgtcgtgc aaaagcgctg cgagcgaccg
    22141 agcttcacct gtcggcattg tacggaagat tgggtcttct ggttcccgat gacaaagatg
    22201 ctgcgcgacg gccacgccca caggcgccca cgacgaactg cggcacacga ctggcgacga
    22261 ccaccgactt gcgggcggct aaatcgctac gttctgccgc tgaagcagca cttgcatcgc
    22321 ttgcgccgcc gagagctgtt ccagcaccag ctcgacggtg ccacgctcgg gaaacttttc
    22381 cgccttcgga tcatcgatca acagccgggc aagctgcggc cacccactgc gggcttgctc
    22441 ggtcagttca cgcagctcac gtcgcagcaa gtacagctcc ggctcgggcg aagtcggggc
    22501 ggaaaacagc acttgtgcca agagccgctc tttttcctcg atgagtcgct tgattcgctt
    22561 ttgctcactc tcggggagct gggtttcaag ttgcagccgc tcgctcagcg ccaaaatctg
    22621 ctgacgcagc tcgctgcggt gctgctgaag tcgcgtctgc tgctgctgaa cctgatcatg
    22681 cagccggttg attcgctgct gccgcttttc ctgcgccagg gtgttcccaa cgagaagctc
    22741 gcggagcatc tgcggctttt ccggccactt gtcgacccac aacttctgcc caacttgttc
    22801 gatcagcgtc gacagccgcg ccaagagccc gacgatttcg ctatcaatcg cttcgatttc
    22861 cgcctgccgt gccgctgcat tgggttcgtt gcgcggcaaa atcaccgtct cggcccgcag
    22921 ctgtggcgcc acagcacttg gcctgggcag aagctgcgtc tctttcccgc tcagcgtcgg
    22981 ctcagcatcg gacttggtct tgggctgatc cccccgtcgg agctgcaccg aacgagccgg
    23041 caaaagcgcc cgaatccgtg tcagaatttc gaccgtttcc gtcgctgtag gccgtcggtt
    23101 cgggttcttt tccagcagct gcgccgccag ctcgtccagc tctcgcggaa tcagcgcatc
    23161 tttgcgcctg tccgacagcc gcggcggcac cacgttcatg tgcccgttca ggatctcttt
    23221 gatatcgcct cgccacggcg gttcaccgac gatggtgtca tgcagcatga tccccagcgc
    23281 atacaaatcc accgacggag aaagctcgcc cgacgcagac atcatctctg gcgcgataaa
    23341 ctccggcgtc ccgatgatct cttcaccgac gcgagtcagc ggtggctgac caatcacccg
    23401 agccacccca aagtccagca ccttcacaaa atccgtctgc ccccccaccg tcgtaatcaa
    23461 aatgttttcc ggcttgatgt cacgatgtac caccccccgt tggtgggcgt gttctagggc
    23521 gcgggccact tgcagtgaga tgtcgacggc ttgcagcgga tgcaggcact tgccggggct
    23581 gctgacgacc agctgataaa gcagcgtgcc ctcgacgtat tccatgacca agtagggctc
    23641 gcctttgtcg gtctggccgt agtcgtaaat gctgacgatg ttggcgtgat cgacgctgga
    23701 cgcgacgcgg gcttcgcgga aaaaacgcgc cagtgcgcgc tcgttgctga cgaactctcg
    23761 acggagaagt ttgaccgcga acgggcgccg cagcacgttg tggttggcca aatagacgac
    23821 gcccatgccg cctttgccaa gtcgccgctc gaggacaaaa cgcccagcga tgacgcggcc
    23881 aatccagggg tcttcgctcg gacgggcgac ttgctcgacg agccgagtgc cgtctttggg
    23941 acaaaacagc ttgtcgtcgc cctgatacac gcttcgacaa gaaggacact cgcgtcgctt
    24001 ggccatcgtc cccagaaccc acgcactgtg ggggatggga catcgtatca cgaccggccc
    24061 gatctgcatg cccgatttct tccgagtgaa cagcgacggt gtcagacccg cgatgtacaa
    24121 aggttcctgt aagcactcaa tcacccccta tcaaaaagga gcggccatga accccaagca
    24181 caccatcctg tcgtcgctga gcaaccacct ggcgggagcg atttcggcgc ttgccaagct
    24241 gtcgcggcgt gtgggcgcaa aggtggtcga ctacgtgctc gtcgcaggac aacatccatc
    24301 gccggttcgc tcgccagaaa tcgcagcctt cacggcactc gcggcggaac gggccaaacg
    24361 gcgggcagcc caaagccaag cagcccccgc cgcagcgctc ctcgcatcag cgacgcttac
    24421 gatgtaattc gacgagtcct acgtcgctag gcggccagcc gctggtgatg cgcgcacgga
    24481 cacacaggca caagcgccag cttttgtcga cgctgccacg acaaaagctg aagcgcaacc
    24541 aagccaagca gactgatgcg ccgcgcaagc tcgctgtcga gcccgaaacc gacgagccca
    24601 agcagaaggg acagcgagag aagagcccct gtcacaccac gcagctgccg tcgccgatag
    24661 agcagcgtcc cgaccacccc aatcgacaac agccacagcg gaccttcgag ccattccccg
    24721 gctgtccaca gcgacgcggc tgccggaagc gcgagcgcca gcagcaccgt cccgacgcag
    24781 tgaatgaagc acagcgccgg caccgccgaa agcagcttgg ctagcacatc gcgccccgac
    24841 gaggaaatcc cagcggtcgt gtcttgaaaa cggacttcat cgggaacgtg tgacatcgcg
    24901 gcctcgtgct ctggtttttg caagatactt gcaactatgt caagtcggcg cggccaaaaa
    24961 atgggcgaaa tacgaagcag tggacgacac ggcggttgac ggacgcgccg cacttgggca
    25021 cttcatgaag catgcaagac aggcagccga tttcgcccca agcggtctac ttcgcgcggc
    25081 atgcgcttct gccgtcggga atgtgcaaaa atgttcgcat cgagctatcc gacgacggca
    25141 gcttttccag cgtaacgcca caggccagcc ccgatggtgc gacggagctg cccggcgtcg
    25201 tgctgccggg aatggtcaac cttcactcgc atgcgtttca gcgcgcgatg gcgggactga
    25261 ccgagcgtgg cgggccgctc accgacagtt tttggacctg gcgcgaagtg atgtatcact
    25321 ttttgcgcag cctcgggccc gacgatgtgg aagcgattgc ggcgcagctt tacgtcgagc
    25381 ttttgcgtgg cggttttact ggcgtcgtcg agtttcacta cctgcaccgt gacccgcaag
    25441 gcgagctgta cgaaaacccc gccgagctgg cactgcgcat ctcccatgcc gcggaacaga
    25501 ccggcatcgg cctgacgctt cttgccagtt tgtatcaaca gggcggcttt ggcgcggagc
    25561 cgctttcgcc gggacagcgc cggttctatc tgtccgacga ccagtttggg caggtgatca
    25621 ccacactgat ggcgcacagg cggcccatcg atcgcatcgg catagcgccg cactcgctgc
    25681 gcgcggtgac accgcaggcg ctggagcagg cgacgcagat catcgaatcc atcgatccgc
    25741 gggcccccat ccacctgcac atttcggaac aaccggcgga ggtagcggcg tgtctttcct
    25801 ggtcacaaaa aacaccgatt gactggctgc tcgatcatgt ggcggtcgac gggcggtatt
    25861 gtctaatcca cgcgacgcat gcgacagaag cggaaatttc gcggctcgcc cggcatgctg
    25921 tcacgattgg actgtgtcca agtaccgagg ccaacctggg ggacggaatc tttgcggctg
    25981 ctgcgcttat cagcggcggc ggacggattg gcattggtac agatagccac gtcggtactt
    26041 cggcgctcga tgagctgcgg cttttggaat acagccaaag actgcggcac ggtcgacgca
    26101 atgtcctggc caagcaagaa ggcgtctcga caggagaatc gctgtggcta gcggcagccg
    26161 gtggcggagc gcaggcatcg gcccagccca ccggcgtcct tgcagccgga tcgcgcgccg
    26221 acttggtggt tctttccgac gacagacctg cactttatgg ccgccacggc agccagattc
    26281 tggatgcggc gatgtttgga cctgaccgcg atgccgtctc ggatgtgatg gtgcaggggc
    26341 gctgggtggt tcgctcgcgt cggcatttcg ctgaagaatc gatcgccgca cgcttccgct
    26401 cgacggtcga gcgactcgcg gcgcgggggc cgtgaagcct gttccgcaga aaccgcccgt
    26461 gatgctaggg tgtacgtctt gggattgatt cgatgcagcg gccctttcgc acattccttg
    26521 gcgtcgatct cggcggaggc aaaggcaaga ccacggcgat ggttcggctg cggctcacgg
    26581 gccaagggga taagccactt gtcgtcgagg actgcggcag tggcaagccc tattttgacg
    26641 accgactgct aaccctcgtc gagcagcaca aagacgacgc tgtgctcgcg gtcgatgcgc
    26701 ccctgacgct gccggtctgt gtacgctgta ctctggcgag ctgcccgagc ctgacccact
    26761 gcagtgtcga tacggttcgt tggtttcggg accgcacgac ccacgaagcc agctcaggca
    26821 agccgcgcta tacgccatat acccagcgag ccaccgaagt gatcctgcac gaagtgcacc
    26881 aaatcctgcc gagagagacg ctgggccaag ggatgggacc gctgacggcg cgagcactgt
    26941 atctgcgaca ggcgctgcgc aagagctatg tgctaaacca aaatctcatc gaggtgtatc
    27001 ccaaagcgac gctgacgcaa ctgtttccgg acccggcccc gccacagcca gcgtcggcaa
    27061 ttcgttatcg cgatgcccaa ggtcgcccgg tgtcgatcga aggcaagccg cttcatccgg
    27121 ggtcgacggt cgcgcgcacc tacaagcggg gacacgggcc cgacgtgcgc aaggatgttc
    27181 tgtcgtcgct gcccgagctt tcgtttgcga ccggacagtg gcgcgaagcc gtgatcaaaa
    27241 acgatcacat cttcgatgcg ctgatctgcg cctataccgg ctatctgtgg gcgcgcgatg
    27301 gctggcagct gcccaaagac ccggtctttg ccgacgatgg ctatatctgg acaccaccgc
    27361 gacgcaacaa ctcaatctga cccaccgttg gtggcgctcg ctcgccacgc cttgccggtc
    27421 acatttcgaa aataaatttc actttgggtg ggcagtagca ggctcgtcca tatacttact
    27481 cgcgatcgac ttgcattcat aaggagtacc catccatgaa gtcttggtat cgtctgctct
    27541 tccttggcac aactgcagtc gcagtggtgg ctgccagttg caatcacgac aatcccggca
    27601 caagtgacct aggtggtgca gacttcgccg tcggttctga catggccggt agcggcagcg
    27661 acgatggcgg cggcgatggc ggcgcggtca gcaccgacat ggccatgccg ctcgcgctga
    27721 ctgcggccag tccaccgacg gggccgacga ccggaaacat cgacgtcacg atcagcggca
    27781 ccggctttct aagcggcgcg tttgtcacca tcgacgggca gagcgcaacg gtgaagtcag
    27841 tgaccagcac cgccatcgtc gtcaccctgc ccgcccggcc cggcaccaaa ggccccgtcg
    27901 ctgtcgtcgt cacaaacccc ggcggcaaga cggtgatgag ctcgacgatc ttcgcctact
    27961 actttggcac cctgagcttc gatactgcgg tcaagaccac cgtcggtggc acccccacca
    28021 acatcgcaat caccgaaatc gaaaacaacg ccaagcctga catcgtcgtc accgacaaca
    28081 gcagcatcgg tcaaattgtc accttgtcgg gcaacggcaa tggcacgttc ctcacccccg
    28141 ccaagtttgc cgccggcatt tcgacctacg ggctcaaagt cctggacatg gatgcagacg
    28201 gctttaacga tgtcgtcgtc accaacgcat ccagcgcacc gtcgggagtc ggcatccacc
    28261 taaacagcaa gaccggcacg ttcccgtcgc gcacgccggt cgtcacaggt gacattccat
    28321 tttctgtcga tgccaaagac ataaacggcg acggctacat cgatctcgtc gtcgccaacg
    28381 gcggcggcag cagcaacaac ctggctttcc ttttgggcaa agtgggcgga acctacgccg
    28441 cgccggtcaa tctgaccgcc gggaccgtgc cccgttctgt ggtgtttgtc gacgtcaaca
    28501 aggacggaaa gaccgacatc gtcgccgcca acgaatcaac cgccaacgtc agcgtcttta
    28561 tcggcgacgg cacgggcaac tttggcaaca agaaagacta tcaagccggt tcggggcctt
    28621 attccgtcgc tgcgaccgat ttcaatggcg acggcaacgt cgatctcatc gtctgcaaca
    28681 gccgcgatgc gacgatcgca cttttgactg gcgtgggcga cggatcgttt aatgcgccca
    28741 agacgacctc gctgggaacg ccgaacacct cgctgccata tcaaatcgcg attggcgaca
    28801 tcaacggcga cggcaggccc gacgtggttt ccgccattta cgccgaccag cagattggca
    28861 tcacctacaa cgccaacggc ttgtttggca ccacgaccaa gctggcagcg agcggcttcc
    28921 cacgcggcgt ggcagtcggt gatctcaatg gcgatggcac caccgacatt gtcagcgcca
    28981 actccaccga cggcagcatc tctatcatcc tcaacaaatc gaagtagcgc cgcgccagct
    29041 tgccggccca ggtgggcctg cctgcgcggg caactacttg tcagcagacg aaaaacctgc
    29101 gatagcttgc gctctcatgc gccagttctc ttccgattcc gccgacgaaa acgtcatcac
    29161 catcgactca gacagcgcac cgcgcatctt gttctatggc gaagactttc tctgtgagga
    29221 cttgcccgtc gggaccaagg tcatttaccc gcgtcgtccg ataagcggtc tgcccaatcc
    29281 ccgcgccgcc attcgctatg ccctgcatca ccccgaagac atggagccgc tgcacgcgct
    29341 gctctttccg gggatgaagg tgacgattgc cgtcgacgat atttcgctgc cgctgccgcc
    29401 gatgcagaag ccggacatcc gcgagacgat ctgctcgatc gttttggaaa tgctggcgga
    29461 ccatggtgtc gacgacatcc acatcatcgt cgcgacgagc ctgcaccgcc ggatgaccga
    29521 gcgcgaaatc cgctacatgc tcggcgacaa aatcgtcgac gcatactggc cggacaatct
    29581 gtataaccac gacgcctgcg accccgaacg gatggtcacg atcggcaaga cccgccacaa
    29641 cgaggtcgtc gagacgaacc gccgcgccat cgaaagcgac ttggtgatct acgtcaacat
    29701 caacctggtg ccaatggatg gcgggcacaa gtcggttggc gtcggtctgt gcggttacga
    29761 aagcctgaaa gcgcaccaca cgccgaagac gattgtcgac tcgcagtcgt acatggaccc
    29821 gaaaaactcg gcgct
//
LOCUS       DAORGN010000039        29799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_579_length_29799_cov_6.178792, whole genome shotgun sequence.
ACCESSION   DAORGN010000039 DAORGN010000000
VERSION     DAORGN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_29799_cov_6.178792"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="PKL17_06645"
     CDS             <1..1954
                     /locus_tag="PKL17_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNK44441.1"
                     /translation="HGVSPLGPEKTLSDVATFQVAMPTDLGATVKRKFYYRAVAGMSM
                     GGIGASMYFFRHPDRFDAVGVLGADPGPDLTYSMGFTRDMFFGGFCTADDEKAGKGKI
                     GQTCPLPRPPIAGQNENVGSFEAMPVQLGEGIGLTLRRSLYLRANRDLVRAIGNWAMY
                     NPIDPYLPPGVPSSTVMLTPDQACKNPVVIKGQKSDPTAKPLYDARFNPEGKWDVITF
                     CDGGEVDGKQGVFDPAKQQTNPVQIALAVDLNANGKRDAGEPVVIQASEPFQDVGLDG
                     LPDQKEPGYDPIRNPDPAGDNYHYLKNPGGTEGNFRYDQGEPYEDSGLDGVKGKGCEI
                     DSGNPGCFDHGEANGKFDYNPGLMKWMDHDPHTLMEKQPLADLAQKDIYYDAGIRDFF
                     NAHISTGYLFGVLAARGLSTQLFGGFPALNGLAPTSEARFSAADVDMAKLGRRLFVRY
                     GNPEISEAKAAETGDGRHAGEVTQVVHRAMILFKFLLTRWPDADTSIQPADDPRLVPK
                     GLSLKLKNGRQTPFSMILPPGYFEPQNQNLRYPVIYIGHGYGMNPEDLGKSIGSLIHS
                     FMSEPDEKQRLPKAILVFIDGVCRPGGEVPTQPLPGEGDLCEEGTFYTDHPQGSFLGD
                     SMLEQLDAYLRANYRLREPSEQTIPL"
     gene            2047..2601
                     /locus_tag="PKL17_06650"
     CDS             2047..2601
                     /locus_tag="PKL17_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44442.1"
                     /translation="MKIESQSKKWFFTGTAFALTLLLSPLAFADATDEEEGTVTGDEF
                     APDWANPDFAAKLSQAQAESRIRAAGISVSSSGNCTNRNNSTCTSFDQINDSTVSGIV
                     TLRSASGCAMTITGGTETGHASGTYSHWNGYKVDIAFSSCIGSYILNNFSYIGKRGDG
                     APMYKSAAGNIYAKESTHWDITYF"
     gene            2667..3128
                     /locus_tag="PKL17_06655"
     CDS             2667..3128
                     /locus_tag="PKL17_06655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNK44443.1"
                     /translation="MPTHPTCPFDPLRSDLVVEGRGLAGRGLFAKVPIPKGTVLGCLT
                     PGTPTVLLLDADGNVSYGQWESAQTIDLVVDNQRLICLVKDFGKDGPRGVDLLNHSCT
                     PNCEVVARLVVKTVRDVPAGDELTIDYLASGITLVKEGIACLCRAGCPTVI"
     gene            complement(3121..3933)
                     /locus_tag="PKL17_06660"
     CDS             complement(3121..3933)
                     /locus_tag="PKL17_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNK44444.1"
                     /translation="MTKPSFQPTPWQTLPQVCLPATPEDRSPRVDLWLFFHEATDHSA
                     PFAAYFPLLTDEERARHDRFVFDRDRRIFLATRALVRCVLAEYTGVDPRDFRFASHRF
                     GKPFVDAPRLSPALSFNLSNTHNLVVCAVTLGDEMIGVDVESLDRASDPLSLADHVFA
                     PAEIAALRSLPVEDQQERFFSYWTLKESYIKARGLGLQLPLSQFAFTLGAAPAPTIAF
                     DSRLADDAAAWSFALLSAFPTHLLAVAKKQRPPLALHACRYVPLKGMIPPVK"
     gene            complement(3970..5235)
                     /locus_tag="PKL17_06665"
     CDS             complement(3970..5235)
                     /locus_tag="PKL17_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44445.1"
                     /translation="MSLLKWKLMLTTLPVPVAAVIVRWVLEHFFKNYMNNYSGVMDFS
                     ALAPVLTGGVFLIGLMLSGTLQDYKESEKIPAELATSLETIEESIGWVCANRPQVNER
                     QMRDILMEVTDELIAWLYRKRSHEQMFIVIEKLNLLLKEFDKLGANLPSVRMTTEIHN
                     LRRTVTRIGVISRTGFLASGYALLDLLVVSVLTLLVVASYKSEFGTLGKYIIIGFVSL
                     IYSYLWRLVRDVDDPFEYSPDGERTGAAEVELFPLTEYQARARDRLAGKTLEERYASA
                     QDPDEEKPDAAPSPALLIGVGVGCLFIGAFVVWLVSRSIGPQGSSAGVTPVPATSAPV
                     RKVEEGAPKQPADKPGEEAAQPGKDEKSDKDEKSDKGTAQNPATAPADKPSSDSAPVR
                     IASPKEDDSDAASDPPKKKRRHHRHKKEE"
     gene            complement(5281..7668)
                     /locus_tag="PKL17_06670"
     CDS             complement(5281..7668)
                     /locus_tag="PKL17_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein kinase/ABC
                     transporter substrate-binding protein"
                     /protein_id="HNK44446.1"
                     /translation="MHRLLLLPPPPPSDGTPPDPRIGATFGKYKVIRLLGKGGMGAVY
                     EGEDAALRRKVAVKFLPDELLNKPDVIERFMREAQVAGGLNHPNIIAVYDVGKDERGC
                     YMVMELLHPKSASSRIKSEGPYHWIVATRIIADCAAALSIAHQNGIIHRDIKPDNILF
                     SHMGGVKLVDFGLVKLVEDDLHLTQSGMLCGSPLYMSPEQASNHAMDQRTDLYSLGAT
                     YYALLAGKPPFHGSGVPQILLSHLTEPTPDPREVNSEIPEACVQIVNRAMAKKPDQRY
                     QTAADMAADLEALLAGIPQRNQSVFTMQEDAPSLLAKANQSQSGALKIPSSSGLRAPA
                     EVGEVAKPPVEPRSGRRAFLAFAALAVVGAGGAIFGLRGRSGKKTTPTGSEGSTTPPV
                     TSGPPIKVGILHSLSGALAVSEHPLADASLLAIDEINAKGGVLGRQLIPVVVDGKSEI
                     TADSAFTRGAEKLLEADKVAAVFGGFGSAGRRVIRPYFEKHDQLLFYPAQYEGIEESQ
                     SLVYTGCTPNQLAVPAVKWCAESLNAKKFFLIGTDGLRAHAINAIVEDHIEQLHGDVV
                     GTHYAVVGESNFAQIVKKIEKVRPDIIINTLVGDSVLSFFRALIEEDITARTLPVLSF
                     TLGENELAQLSEPVLAGHYVARTRFPPIADSKVEPFATRFQRKYGEHRPVSETMEAAY
                     YGVYLWAAAVQRAGSEEVNRVRLALKGQEFSLGNVRVRVDPSNQHIWKIFEIGQVRSD
                     NKIDVIKTGDAPIPPLPFPGPRTHAEWQSFADALYEKWGGNWANPAKPKQKKSKK"
     gene            complement(7715..11290)
                     /locus_tag="PKL17_06675"
     CDS             complement(7715..11290)
                     /locus_tag="PKL17_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK44447.1"
                     /translation="MTSHATQAPDSLVLGIDGFVYADLFSPYGLRRLHDCWRAQVGRE
                     HPALLDRYDAYQKGEPLSDKDLSALLLELAATVSRFLAQLVPDIATPLAALRDETATD
                     LALFRYKDEIIKRQADKLRLSADQAESATVEGEALLSRHGLSSLPSERDLARLGLRLL
                     DDDQAAELAILVRWLAAQKQALLDAGHLGFRLPKATDFSQLVPLHRPDPNIPEALCGH
                     EEHQRRRDGFALTDPRKSRLQVLAEVDYCMYCHERSKDSCSKGMRDKNGAVKQNPLGA
                     PLTGCPLGEKISEMHTLRRQGDPLGALALVMLDNPMCAGTGHRICNDCMKACIFQKQD
                     PVNIPEVETSVLTDVLGLPWGVELYGLLLRWNPLHRTRPYPLAYNGRKVLVVGLGPAG
                     YTLCHYLLREGFAVCAVDGLKLEPIDRALAGDLASGVVPKPVRDFSRLKAPLDARVLT
                     GFGGVSEYGITVRWDKNFLELLYLSLSREPLLQLHGGVRFGGTLELDDAWQLGFDHVA
                     LATGAGKPTLVEMEGNLCRGIRQASDFLMGLQLTGAFKRDSLANLQVRLPAVVIGGGL
                     TAIDTATELAAYYVVQVEKMLDRWESLVPELGEAALTARFDREEREILDELLSHGRAF
                     RAERQAAAAQGRSPYFAPLIARFGGVTLVYRKSLLDSPAYRLNHEEVAKGLEEGIRYI
                     EKLSPKVALTDAFGALRAVQFERQAQNGERYSGTGEVVELAARTLCIAAGTSPNTMYE
                     KEHPGTASLGRGGFFAPHRADWRDSQLALVPDANGFFTSYLRDGKVVSYYGDNHPRFA
                     GSVVRAMASAKQGHSQVSALFAADLARAKQEVQRDSPSEEQTRRDLAWQALRTRLFDE
                     LRAHVVSVERLTPNIVDVRVRAPWAARKFEPGQFFRLQNFETHAKVVAGSRLSMEGIA
                     LTGADSDKESGILSLIVLEMGGSSSLCQRLVPGEPVVVMGPTGTPTEIPKNETVCLVG
                     GGLGNAVLFSIAKALKANGCQVLYFAGYKQPQDVFRQDMIEAHTDEVVWCCDVPPPSG
                     AALRSRRSGDKLFVGNIVAALLAYAKGDLGPPRFDLQQVSRFIVIGSDRMMAAVAQAR
                     HTILREHVNPRAVAIGSINSPMQCMMKEICAQCLTRHRDPQTGQESVVYTCVNQDQSL
                     DAVVWPHLAARLRQNSTQEKLTALWIAHLTELAPPPALPSPVERG"
     gene            complement(11298..11993)
                     /locus_tag="PKL17_06680"
     CDS             complement(11298..11993)
                     /locus_tag="PKL17_06680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNK44448.1"
                     /translation="MISTFAALALPTLLAAQAATPPPPVGPTTPPLVLEQSLVGIKGQ
                     GALQATLELVSEGKPLGAVHCDLFADKAPQAVAAFVGLARGLVAFKDPHPQRTGWQKK
                     PFYDGLPVHRVIGEFLIQSGDPKCFGDAQCMFTPGSGDPGFALPDEVRPELRFDQGGV
                     LAMASRGPNTAGSQFFITVKAAPWLSGQHTIFGQCDNLPLLDQVSRLETYSNDAPKVP
                     VVIQRLTVTRKNK"
     gene            complement(12020..12529)
                     /locus_tag="PKL17_06685"
     CDS             complement(12020..12529)
                     /locus_tag="PKL17_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNK44449.1"
                     /translation="MPAFRSSRVTLPALVLAASSLTWLGCSKPPTPQPVSQSPAQKPT
                     VAFYPSSGNPPWPVTVELAKNDQERTRGLMFRRELAPSSGMLFLFEAPEIRRFWMRNT
                     LIPLDMIFLNERKIVVGIEENTIPHDETSRGPDQPAQYVVEVAGGEARKHGITVGSRA
                     EFLHVDGAK"
     gene            complement(12536..13096)
                     /locus_tag="PKL17_06690"
     CDS             complement(12536..13096)
                     /locus_tag="PKL17_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44450.1"
                     /translation="MARLVDLFALAIVAFLLLLPQPTVVFTPGFAGDKSDIDRIAVLE
                     DALYRKQDDVETAVELARAYLRIEQPAWAVATLRPYGKADSYQVHQVLATAYATVLRF
                     DLGLAEAEAGLAVCESQGPRCPEVTQIRLGYLANLMRENAQKGIDANTDPVAAKRMVQ
                     AALRATKAQTPTTPPPPAKPAPAAKP"
     gene            13202..17263
                     /locus_tag="PKL17_06695"
     CDS             13202..17263
                     /locus_tag="PKL17_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44451.1"
                     /translation="MSVLCRMPKRSRSVGRALLLGLLLPLLPSPVALADDWQVTRSEF
                     DPRIVNELKDQVRKSPDDSGLFRRLVGLYRRHSSLEKLQAELADRAQRSGQATDLYLV
                     AQVARERGQPGDAAKLLEEAVQQGLDEAKAALLLSELAQKKTPPDVAEARKQLGRALS
                     LLRPSDARRRPALRRQVDLSLQAGDTARAEAELRDLMKSATGTESTRLAQELAELLAR
                     AGKSKESLSEYRALAEKQAGESKVQSLLRCGELEESLRDDNAAIATYRQAQKLLPAGH
                     HLQRDVSEHLIALSRKRDELVPFIKQFEADHPQAQRSFSDWELLGRLYDERGDTAAAL
                     LAYRGALRKDPHHIDVRRRLIAILERGGVTAEVLSEYEQLIAQAPGDSRGYLELAERQ
                     WKVGQKPKALLTLRRAAARFAADPSLHSALAELYSRFGEADLALQESELLVRLEPREE
                     SYIINLGELYWARGRKERADEVWKRLLTMGTSRAQGQARLADVYAEHNLMSQALDLYQ
                     KAVSAEPNNLQLRRGLAQAYERLSRGRDAMVTWEQIYFAAKSPSERLLQIEARKQLAS
                     LVGKESRLSAQLYTWQRRLAAQLGQQTQTLQPDTLMLGLLVAETQQQAGKLGDAEQVL
                     SQLRVRLPDGPQLAEVLLSLCQVLKAQRKLEEAIAVLKQAASLLPDRQRELYAQLADL
                     SMQSYRDGDAIRYAEQAVADASGELSLGEILERRDEVPRAMAAYKRAMERDPRLFRAY
                     MALARLHVQRGELHEAAALYRDVVRRTPQEELVLEAGRKAIDLHEYLGSLGELLKELS
                     PLAYSVVPRPVYRRLLLFLYERYAAPLIALSRSGDAGAQAELVRLGQGGLKPLTETLT
                     EGDTTEQRLAVSLLAELRNPSAVPSLLNLVMAGLPLPGQEPRIVLHGSTGSGGVPGGV
                     PGGVSGVRTGADIDLRVDALLAVARLSDPQSVPGLLRLTRSREKQVRLAALYGLLRLA
                     DKLDRAQLGQLDPLLADAAPGCRALAALLFGRVSELSGQPIAPKIRSQLVAALETRRV
                     RNDDADELFASAAAQALGRARDRAAVPLLIDVLRLGNDEVERQAAWALGRTGDPRALP
                     ALVRAVFGKQPALRMTAAAALASLSTGPQPQPADVLPSEKRGLDGLDVARFLSDIAGQ
                     GALGISKPAWLDSPGELSGALRDALDDTPALALRTLDDLLGSTSGPPQVLRLGPLETW
                     LPAGTDASGVRESVAAALLPALRKLGLPDGVASSPLLQPAALRVLGQIAQTRSPIAPK
                     AAAVLRDVLLSRASTDVLLLSASLYAKVPPSQREAQPVLDALLQQPDRWLRLQTLRLA
                     AEPDARPLLSASAIVKASLDPDGFIREAAQRLK"
     gene            complement(17580..18422)
                     /gene="mutM"
                     /locus_tag="PKL17_06700"
     CDS             complement(17580..18422)
                     /gene="mutM"
                     /locus_tag="PKL17_06700"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNK44452.1"
                     /translation="MPELPEVETVCRGLRGFLCQQVLSDVVVRRRDLRVPIPPDFEQK
                     VRGRTVIDIDRRAKYIVCRLDSGDAVLIHLGMSGRINGFVAADPKRPLAPAKHDHVLF
                     HTQSGTLVSFCDPRRFGVMTLAKTDKLAQHLLLKDLGIEPLSHELTPSWLTQALRKKR
                     VPIKVALLDQALVVGIGNIYASEILHRAHILPQRLACELSPREVKAVCASIPVILRKA
                     INAGGSTLRDYVQASGELGGFQNLFAVYDRESEPCKRRGCAGTIVRIVQAGRATYFCP
                     RCQH"
     gene            complement(18616..19125)
                     /locus_tag="PKL17_06705"
                     /pseudo
     CDS             complement(18616..19125)
                     /locus_tag="PKL17_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(19458..21026)
                     /locus_tag="PKL17_06710"
     CDS             complement(19458..21026)
                     /locus_tag="PKL17_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK44453.1"
                     /translation="MSKPPLSAASKPVLFALGAWVAMACPRFACALGESAPIDINKFH
                     PAPGTQKLVTLDLAEVDPHLTFVPQLFVHYARRPLVYTLGGVPAADLVQNRLTGDISV
                     SVSFLRRLQVSLSLPVTFWQTGELPQNGFLDPKDPTSGRTVKRQFAKDGLAQAGQEDL
                     RFSVKAVLYRYRQNDDPEKIGFGLGIAGDLTVPTGNADSFMGSRLPTFAPRLLAHLDY
                     RRLQVAAFVGSIFSSTERFYNAETQHSFLFGLGLQLQLRRSPTAPISLVTEAWGSTPF
                     VVTDRLANTPAELTVALKAAYHGFTFFLGGGPGLSPGVGVPDWRAYLGMSYAGTYVKR
                     PPPPPPPPPPPPPPPPPKKPVEPPPPPPPPPDLPPSCEGEDCPDQVRRPAHVEVKGQK
                     LVLSERIFFDFDRDTIKPISYPVLDEVVRVLKARPDIKRMRVEGHTDNKGRDQYNLDL
                     SRRRAQSVMVYLVDHGIEPWRLSSAGFGLRCPIVPNDSPDRRAQNRRVDFIITEQEGV
                     TLDPPKCPLTLISR"
     gene            21228..23858
                     /gene="leuS"
                     /locus_tag="PKL17_06715"
     CDS             21228..23858
                     /gene="leuS"
                     /locus_tag="PKL17_06715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNK44454.1"
                     /translation="MNPSQTAEASASLPEKYDPQSVEPRWQAEWQRQGAFLAPPAVDS
                     SGKPDPRPKYFIMEMFPYPSGRLHMGHVRNYSIGDVLARAYRMKGHRVLYPMGWDAFG
                     LPAENAAIKAKVHPRDWTVKNIESMKGPLGRLGQSYDWSHELSTCEPEYFIHEQRIFI
                     EMWKRGLAYRKGALVNWCTSCATVLANEQVEDGLCWRCQSVVIQRELTQWFLRITEYA
                     QQILDDIEQLRGKWPDKVLHMQENWIGRSEGARIRFPLVQPIADVTEIEVFTTRPDTL
                     FGATFMSIAAEHPLALRLCAGTSREAEVTAFVNKVRAEDKTRRGADDYVKEGCFTGAC
                     VVNPANGRQIPVYIANFVLMDYGTGAVMAVPAHDQRDFEFAKKYALPIEVVIQPDGTA
                     LVASELSAAYTERGQLLNSGEFSGQDNEAAKKNITAWLAQKGQGGPTVSFRLRDWLVS
                     RQRYWGCPIPMVECPQHGYQPVPDAELPVQLPTDIEFVPSGQSPLATHPTWKNATCPV
                     CQGPARRETDTMDGFMESSWYFLRYTAPTFAGGILDEAGKTFLPVDQYVGGSEHATKH
                     LIYSRFFTKMLSDWGVLPKTLREPFVRLLTQGMVLKDAYRCAEHDYLYPEEVQDGRCA
                     KCGKDAQYLRQMKMSKTFRNVVEPMALVEKYGADTARLFCLFAAPPDSVLEWNEAGVE
                     GCSRFLGRMYRLVQRFVSLPEAGGTPPSDSAAADLRRVTHKTIRRVTTDIFERQQFNT
                     AIAAIMELSNAITDFAATQTVQSAALREAVETAVQLLGPMAPHICEELWQALSHKTLL
                     ALSAWPVFDEAAAKDDKVTVVVQVNGKKRAELQVAADSSEDVIQQAALSDPGVKQFTD
                     GKSIKKSIYVKGRLLNLVVA"
     gene            23945..26224
                     /locus_tag="PKL17_06720"
     CDS             23945..26224
                     /locus_tag="PKL17_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK44455.1"
                     /translation="MDARTILLISVALNVPILMFMIQGRLRRADIPGQTEWIGGILLV
                     WLASLLMLLRGSIPDFFSIALGNACWLFCLLAFTTGTYKFLELPSRLPTSVYVMCTVS
                     AILIGLFAYVPSPWPRFPAINIRVVVASAAGALLCVSVLLGMKKTAPRLKPCVEILQL
                     PVWGFALVSLLRLIATLVAPFHGLSPLQGSAGQSLLVFGLLVVDLMVAWAMVYANNRR
                     LDIERREKVAALADSETHIRRFLEAAPVALFSMCSDGSILYANGRFTKQLGYPLAEIP
                     TLDAFFEHVFPDRGYRDAQRTKWQALLSPGDKNERTELRFESALRCKDGQERTFGVQA
                     SAMGDEVLLTLFDLSTHKEAEQALRFAKEEAERAMRSKSLFLANMSHELRTPMNAILG
                     LSQLARECLADKNELRETRHFLAQIHSSAGALLGILNDILDVSKLEAGRMELEQVPFD
                     LHAVIDQQELLHGQKVKERGLTFSITISPAVPRFLVGDQLRLSQILTNLLSNAIKFTE
                     RGSVSLQIEPMATEPAPDKDAPMHLRFVVADTGIGMNEAQVAALGQPFVQADASTTRK
                     FGGTGLGLYISKQLVGLLGGRLEVQSQVGQGSRFVLIAPFLQVPASRVRPLPSDSDRS
                     AQFRPLPLRPLRVLIVEDNPINQLVAQRFLEKLGITTELAQNGEQALGLLRAQRDIDL
                     VLMDVQMPVMDGLEATQRIRGELGLVLLPIVAMTAHAFPEERERCLAAGMNDHVAKPI
                     SAAGLAEVIARWVPVDPAH"
     gene            26302..27120
                     /gene="recO"
                     /locus_tag="PKL17_06725"
     CDS             26302..27120
                     /gene="recO"
                     /locus_tag="PKL17_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNK44456.1"
                     /translation="MRKRSGPKEATPCLVLRVVDYGDADRIVQLLTAAHGKVSALARG
                     ARSSRRRFGAALSSFGFGEALLEERRGQDLWILSDLHVSRGFPNLPLELSRFGHAAYA
                     CELCLHLCPPHVPEPEVLRLLVTYLDYLDGLPIADKPSPLALRVFELQLLAAVGLALQ
                     LDDCAACGAQVPPAPLVPLDVKRGGVLCELCFLGPVPLHLGPQGGGLPQPLRDSLRML
                     GGLQLDPEALSSVSIDVQGHAKARELLQSVIQNHLGHGLRAVEFITKMNAFQLP"
     gene            complement(27182..28003)
                     /locus_tag="PKL17_06730"
     CDS             complement(27182..28003)
                     /locus_tag="PKL17_06730"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNK44457.1"
                     /translation="MSIALFQTLCVVIVSLTLVTMARSTPWKPLLSDYFALAVAGYLG
                     EESCITLYHFYQYSPAWNARLVDVPLLVPLIWPLVVLSARQVGQALWPDAKSPLARAA
                     ITAALVTFDASLMEVVAVRAGLWSWAEPGHLQVPLIGILGWGYFALGAELVLGQPWRA
                     RRLWLLLLAPLVTNALIVGSWWICFRHALRGDLGQASVSAMAGLGVLSLVAVLWQRRR
                     GHCIPLAIAGPRMIAATLFVALLLATAPRDPNLWLQTLALALPYLAATSFRPAPA"
     gene            complement(28025..28252)
                     /locus_tag="PKL17_06735"
     CDS             complement(28025..28252)
                     /locus_tag="PKL17_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNK44458.1"
                     /translation="MSKKKHPVKAHITGNIWKITTTVGAQVNEGDTLVIMESMKMEMP
                     VESEVEGTVVEIVTKEGVPAKEGEILLYIEG"
     gene            complement(28275..>29799)
                     /locus_tag="PKL17_06740"
     CDS             complement(28275..>29799)
                     /locus_tag="PKL17_06740"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNK44459.1"
                     /translation="SPNEVSAMKKQLSLTRRFFPLLCASGLALSCGGIDPNANDNMGG
                     DNPPKVNPFEAKPECSGDKVDFKKGSRGLVVSNLEIAASGQGLDLDGDGKADNKLGAI
                     ASLANPELEGTFKEKHDVVIPMEFFGSTSGDSMCTKFAMYLGQFLKDRDKDGKDNTWE
                     TDNGVAKGDCNDYDKNVHAGAPEVAGNRMDDDCDGLADNPTQKKPADDASAKMDLDGD
                     GVTVAEGDCDDRATSPMVPTDTGMVPLAKLRHPAKAAMSISAGVERCDGIDYNCDGIP
                     DNAAQCDPFGDANVSIDIQKLSLDSQSQPILTFKNGQVKANKLTAGPSVFRVTVPISD
                     QQLELELSGARVEATFRNAGNLTYLDNAVLGGVLEVLSLARLDKIMVKGFLTPPQSLF
                     DAVFANGALAEVLRLKKDKDGHYLPDMDVDGDGLETFWASDPEKTPALVDTCKDGDGT
                     VIKNGDTTVQNDDPKKRCVFAKDKKGNYRFVDGISAALKFKAVPAKLGDVVDTMVGK"
BASE COUNT         5407 a         9172 c         9473 g         5747 t
ORIGIN      
        1 gcatggcgta tcgccacttg ggccggaaaa aacgctgtcc gacgtggcga cattccaagt
       61 ggcgatgccg accgatctgg gcgcgacggt gaagcgcaag ttttactacc gcgccgttgc
      121 tggcatgtcg atgggaggaa tcggcgcgtc gatgtatttt ttccgtcatc ccgaccgctt
      181 cgatgccgtc ggtgtgctcg gggccgatcc gggaccggat ctgacgtaca gcatgggttt
      241 tacccgcgac atgtttttcg gcggtttctg cacggcagac gatgaaaaag cagggaaggg
      301 caaaatcggc cagacctgcc cgctgccgcg tccgccgatc gccggtcaga acgaaaatgt
      361 tggttcgttt gaagcgatgc cggtgcagct cggtgagggc atcggcctga cgctgcgtcg
      421 cagcctgtac ctgcgggcga accgcgacct ggtgcgcgcg attggcaact gggcgatgta
      481 taacccgatc gatccgtatc tgccgccggg cgtgccgtct tcgacggtga tgctgacgcc
      541 ggatcaggct tgcaaaaacc cggtggtgat aaaaggacaa aagagcgatc cgacggcgaa
      601 gccgctgtac gacgcgcgct tcaatcccga aggcaagtgg gatgtgatta cgttctgcga
      661 cggcggcgag gtcgacggca aacagggcgt gtttgatccg gccaaacagc agaccaatcc
      721 ggtgcagatt gcgctcgcgg tcgacctaaa cgcaaacggc aagcgcgacg ccggtgagcc
      781 ggtggtgatt caagcgagcg agccgttcca agatgtgggt ctcgacggtc tgcccgatca
      841 aaaagagccc ggctacgatc cgattcgcaa ccccgatcca gcgggcgaca actaccacta
      901 tctgaagaat ccgggcggca ccgaaggcaa cttccgttac gaccaaggcg agccgtatga
      961 agacagcggt ctcgacggtg tgaagggcaa aggctgcgaa attgacagcg gcaaccccgg
     1021 ctgttttgac cacggcgaag caaacggcaa gttcgactac aaccccggcc tcatgaagtg
     1081 gatggatcac gatccacaca cgctgatgga aaagcagccg ctcgccgacc ttgcgcaaaa
     1141 agacatctac tacgacgccg gcatccgtga ctttttcaac gcgcacatct cgacgggtta
     1201 tttgttcggc gtgctggcag cgcgcggtct gtcgacgcag ctttttggtg gtttcccagc
     1261 gctaaatggc ctggctccca cgtcggaagc tcgtttttca gcagccgatg tcgacatggc
     1321 gaagctcggt cggcggctgt ttgtgcgcta tggcaatccg gaaatttccg aagcgaaagc
     1381 ggcggaaacc ggcgatggtc gtcacgctgg cgaagtgacc caggtcgttc accgcgcgat
     1441 gattctgttc aagttcctgc tcacgcgctg gccggacgcc gatacgtcga ttcagcctgc
     1501 cgacgatccg cgccttgtac ccaaaggtct gtcgctcaag ctcaaaaacg gtcgtcagac
     1561 gccgtttagc atgatcttgc cgccgggcta ttttgagccg caaaaccaga acctgcgcta
     1621 tccggtcatc tacatcggcc acggctacgg catgaacccc gaagacctgg gcaagagcat
     1681 cggcagcctg atccacagct ttatgtccga gcccgatgaa aaacagcgtc tgcccaaagc
     1741 gattctggtg ttcatcgatg gtgtctgtcg tcccggtggc gaagtcccga cgcagccact
     1801 gcccggtgaa ggcgatctgt gcgaagaagg cacgttctac actgaccatc cgcagggcag
     1861 cttcctgggc gattcgatgc tagagcagct ggatgcgtac ctgcgggcca actatcggct
     1921 gcgtgagccg tcggagcaga cgattccgct gtagcttctg ctttcgcgcg gtggggggac
     1981 agacgtccga aaatgaaacc ctacggatgc ctcttattgg catccctttt ccgtcaagga
     2041 ggtcccatga agatcgagag ccagtcgaaa aagtggtttt ttaccggcac tgcctttgcg
     2101 ctcacgcttc ttttgtcacc gctcgcgttt gccgatgcca ccgacgagga agaaggcacc
     2161 gtgaccggcg atgaattcgc gccggactgg gccaatcctg actttgccgc caagctttca
     2221 caggcccagg cggaatcccg gattcgcgcc gctggcatca gcgtgtcgtc gagcggcaac
     2281 tgcacaaacc gcaacaactc aacttgcacg tcgtttgacc agatcaacga cagcactgtg
     2341 agcggcatcg tcaccctgcg cagcgccagc ggctgcgcaa tgaccatcac cggcggcacg
     2401 gaaaccggcc acgcatcggg aacctacagc cactggaatg gctacaaggt cgacatcgcg
     2461 ttttcgagct gcattggttc gtacatcctg aacaactttt cgtacatcgg caaacgcggc
     2521 gacggcgcgc cgatgtataa gtccgccgct ggcaacatct acgccaaaga gagcacgcac
     2581 tgggacatca cgtacttcta gtctgcgctt ctctgtgacg acggcatcgt ccgtcggtcc
     2641 atcatcgctg ccagctctct tcgcttatgc ctacacatcc aacatgtcct tttgacccgc
     2701 tgcggtccga tcttgtcgtc gaaggcagag gtcttgccgg tcgtggtctg tttgccaaag
     2761 ttcccattcc caaaggcacc gtgctcggtt gtttgacgcc gggcacgccg acggtgcttt
     2821 tgctcgatgc cgatggaaac gtcagctacg gccagtggga atccgcccag accatcgacc
     2881 ttgtcgtcga caaccaacga cttatttgct tggtcaaaga ctttggcaaa gacggtcccc
     2941 gcggcgtcga tctgctaaac catagctgca caccgaactg cgaagtggtg gcccgtctgg
     3001 tcgtaaagac ggtgcgcgat gtcccagccg gcgacgagct tacgatcgat tatctggcgt
     3061 ccggaatcac gctcgtcaaa gaaggaatcg cttgcctgtg tcgggctggc tgtccgacgg
     3121 ttatttgacc ggcggaatca ttcctttgag cggcacatag cgacaagcgt gcagcgccag
     3181 cggcgggcgt tgttttttgg cgaccgcgag aaggtgcgtc ggaaacgccg aaagaagcgc
     3241 aaagctccac gcggcggcgt cgtcggccag ccttgaatca aacgcaatcg tcggcgcggg
     3301 tgcggcaccc agtgtgaaag caaactgcga cagcggaagc tgaagcccca gaccgcgcgc
     3361 cttgatgtag ctttccttga gcgtccagta ggaaaagaag cgctcttgct gatcttcgac
     3421 gggaagactg cgcagtgccg caatttccgc tggcgcaaac acatggtcgg cgagcgacag
     3481 tggatcgctg gctcgatcga gcgattccac atcgacgccg atcatttcat cgcccagcgt
     3541 gaccgcacag accaccaagt tgtgggtgtt tgacagattg aacgacaggg caggggaaag
     3601 ccgtggcgcg tcgacaaatg gcttgccaaa gcggtggctg gcaaagcgaa aatcacgcgg
     3661 atcaacgcca gtgtattccg ccagcacgca gcgaaccagt gcgcgggtgg ccaaaaaaat
     3721 gcgccgatcg cggtcaaaca caaagcggtc gtggcgtgcg cgttcttcgt cggtgagaag
     3781 cggaaaataa gccgcaaacg gcgcgctgtg atcggtcgct tcgtggaaaa acagccacag
     3841 atcgacgcgc gggctgcgat cctctggcgt cgcaggcaaa catacctgcg gcagcgtctg
     3901 ccagggcgtt ggctggaagc tgggcttggt caacggacac cgacctgcgg ctcgaaagcg
     3961 agccggcgcc tattcttcct tcttgtggcg gtggtggcgg cgctttttct tgggagggtc
     4021 gctcgccgca tcgctgtcgt cctctttggg actggcgatg cgcaccggcg cgctgtccga
     4081 gctgggcttg tccgcgggcg cggtcgctgg gttttgtgcc gtgcccttgt cgcttttttc
     4141 gtctttgtcg cttttttcgt ctttgccggg ctgcgctgct tcttcgccgg gcttgtccgc
     4201 gggttgtttg ggggcgcctt cttcgacttt gcgaaccgga gcactcgtcg cgggaaccgg
     4261 cgtcacacct gccgagctac cttgcggacc gatcgaccgg ctgacaagcc acaccacaaa
     4321 cgccccaatg aacagacagc cgacgccaac cccaatcaac agggccggac ttggcgccgc
     4381 atccggcttt tcctcgtcgg gatcttgcgc acttgcatag cgttcttcca gcgtttttcc
     4441 cgccagtctg tcgcgtgcac gcgcttggta ttcggtcagc ggaaacagct caacttctgc
     4501 ggcgccagtg cgctctccgt cgggagaata ctcgaacgga tcatcgacat cgcgcaccag
     4561 ccgccacagg tagctgtaga tcagcgacac gaagccgatg atgatgtact tgccgagcgt
     4621 gccaaactcc gatttgtacg acgccacgac gagcagcgtc agcaccgaca ccaccagcag
     4681 atcgagcagc gcataacccg acgccaaaaa cccggtccgc gaaatcacac caattcgcgt
     4741 cacggtacgc cgcaggttgt ggatttccgt ggtcatgcgc accgacggca gattcgcgcc
     4801 cagcttgtcg aactctttga gcagcaagtt cagtttttca atcacgatga acatctgctc
     4861 gtgcgaccgc ttgcgataca gccacgcaat cagctcgtcg gtgacttcca tcaaaatgtc
     4921 gcgcatctgc cgctcgttga cttgcggacg gtttgcgcag acccagccaa tcgactcttc
     4981 gatcgtctcc agcgatgtcg caagctcagc aggtatcttt tcgctttctt tgtagtcttg
     5041 cagcgtcccc gacagcatca ggccgatgag gaacacaccg ccagtcagca ccggagccag
     5101 cgccgaaaag tccatcacgc cgctgtagtt gttcatgtag ttcttgaaaa agtgttcaag
     5161 aacccaccgc acaatcaccg ccgcgaccgg gaccgggagc gtggtgagca tcaacttcca
     5221 cttgagcaag ctcatcgccg cctcaacttg ctgcgtggga gaccgcaagg ccgccgcgcg
     5281 ctacttcttt gatttcttct gtttgggctt tgccggattg gcccagttgc cgccccattt
     5341 ttcgtacagc gcgtcggcga agctctgcca ctccgcatgc gtgcgcggcc ctgggaaggg
     5401 caggggagga atcggtgcgt cgccggtctt gatcacgtcg attttgttgt cgctgcgcac
     5461 ctggccgatc tcgaaaatct tccagatgtg ctggttgctc ggatcgacgc ggacgcggac
     5521 attgccgagc gaaaattctt gtcctttgag cgcgagccgg acgcgattga cttcttcgct
     5581 gccggcgcgc tgcaccgccg ccgcccacaa atacaccccg taataggccg cttccatcgt
     5641 ttcgctgacc ggacggtgtt cgccgtattt gcgctggaag cgggtcgcaa acggctccac
     5701 tttcgagtcc gcaatcggcg gaaagcgcgt ccgtgcgacg tagtgtccgg cgagcacagg
     5761 ctcggaaagc tgcgccagct cattttctcc gagcgtaaac gacagcaccg gcagcgttcg
     5821 cgccgtgatg tcttcttcga tgagcgcgcg gaaaaacgac agcaccgaat cacccaccag
     5881 cgtgttgatg atgatgtcgg ggcgcacttt ttcgattttc tttacgatct gcgcaaagtt
     5941 cgattcaccg acgactgcat agtgggttcc gaccacatcg ccatgcagct gttcgatgtg
     6001 atcttcgacg attgcgttta tcgcatgggc gcgcagaccg tcggtgccga tcaaaaagaa
     6061 ctttttggca ttgagcgact cggcgcacca cttgaccgcc gggaccgcga gctgattcgg
     6121 cgtgcagccg gtgtagacca ggctctgcga ctcctcgatg ccttcgtact gcgccggata
     6181 gaacagcagc tgatcgtgtt tttcaaaata gggccggatt acccgccgtc ctgccgagcc
     6241 aaagccaccg aacaccgccg cgactttgtc ggcttcgagc agtttttcgg cgccgcgcgt
     6301 aaacgcggaa tctgccgtga tctctgattt gccatcgacg accaccggga taagctgccg
     6361 tccgagcacg ccgcctttgg cattgatttc atcgatcgcc aaaagcgatg catcggcgag
     6421 cgggtgttca ctgaccgcca gcgcgcccga caacgagtgc aagatgccga ctttgatcgg
     6481 cgggccgctg gttactggcg gtgtggtgct gccttcgctg ccggttggtg tcgttttttt
     6541 tcccgatctg ccgcgcagac caaagatcgc accgccagcg ccgaccaccg ccagcgccgc
     6601 aaaagccaga aacgcccgcc gccctgatcg cggctcgacg ggaggcttgg cgacttcacc
     6661 gacttcggca ggcgcgcgaa gccccgagct cgacggaatt ttcagcgccc ccgactggct
     6721 ctggtttgct ttggccaaaa gcgatggcgc gtcttcctgc atcgtgaaga ccgactggtt
     6781 gcgctgcggt atccccgcca gcagcgcttc cagatccgcc gccatgtcgg ctgccgtctg
     6841 gtagcgctga tccggctttt tcgccatcgc gcggtttacg atctgcacgc acgcttcggg
     6901 aatttcggag ttgacctcac gcgggtccgg cgtcggctcg gtcaaatgac tgagcaggat
     6961 ctgcggcacg cccgagccat gaaacggcgg tttccccgcc agcagcgcat agtacgtcgc
     7021 ccccagcgag tacaggtcgg tccgctgatc catcgcatgg tttgatgcct gttccgggct
     7081 catgtaaagc ggactgccgc acagcatgcc gctctgggtc aggtgcaggt catcttcgac
     7141 gagcttgacc agcccaaagt ccaccagctt caccccgccc atatgcgaaa acaggatgtt
     7201 gtccggctta atgtcgcggt ggatgatgcc gttttggtgg gcgatggaca gcgcggcggc
     7261 acagtcagcg atgatgcggg tggcgacgat ccagtggtac ggcccttcgc tcttgatccg
     7321 gctcgatgcg ctcttggggt gcaaaagctc catcaccatg tagcagccgc gttcgtcttt
     7381 gccgacgtcg tagaccgcga tgatgttggg gtgattcaga ccgcccgcga cctgggcttc
     7441 gcgcatgaag cgctcaatca catccggctt gttgagcagc tcgtcgggca ggaacttgac
     7501 cgcgactttg cggcggagcg ccgcgtcctc gccttcgtag acggcgccca tgccaccttt
     7561 gccgagcaga cggatgacct tgtattttcc aaacgtcgcg ccgatgcgtg gatcaggggg
     7621 cgtgccgtca gatggcggcg ggggggggag gagcagaagg cggtgcacct gtttcggtca
     7681 cgataaaaca tcatcgacga ctttttgcac tgggtcaacc gcgctcgacg ggcgagggca
     7741 gggcaggggg cggcgcaagc tcggtcaggt gggcaatcca cagcgccgtc agcttttcct
     7801 gtgtgctgtt ttggcgcagt cgtgccgcca agtgtggcca caccaccgca tcgagcgact
     7861 gatcctgatt tacgcaggta tagaccacgc tttcctgtcc ggtctgcggg tcgcggtgcc
     7921 gggtcagaca ctgggcgcaa atctctttca tcatgcactg catcggcgag ttgatgctgc
     7981 caatcgccac cgcacgcgga ttcacatgct cacgcaggat ggtgtgccgc gcctgcgcaa
     8041 ccgccgccat catccgatcc gatccgatca cgatgaaccg gctgacttgc tgtaagtcaa
     8101 agcgcggcgg tcccaagtcg cctttcgcat acgccagcag cgccgccacg atgttgccga
     8161 caaacagctt gtcgccggac cggcgcgagc gcagcgcggc accggacggg ggcggcacat
     8221 cgcagcacca caccacttca tcggtgtgcg cttcgatcat gtcttgccgg aacacgtcct
     8281 gtggctgctt gtagccggcg aaatacagca cctggcagcc gtttgctttg agcgccttcg
     8341 caatcgaaaa cagcaccgca ttgcccaggc cgccgccgac caagcagacc gtttcgtttt
     8401 tggggatttc cgttggagtt cccgtcggcc ccatcaccac caccggctcg cccggcacaa
     8461 gccgctgaca caggctcgag ctgccgccca tttccagcac gatgagcgac aagattcccg
     8521 actctttgtc ggaatccgcc cccgtcagcg cgatgccttc catcgaaagc cggctgccgg
     8581 ccaccacctt ggcgtgcgtt tcgaagtttt gcagccggaa gaactggccc ggttcaaact
     8641 tgcgcgccgc ccacggagcc cgcacccgga catccacgat gtttggcgtc agccgctcga
     8701 ccgatacgac atgggcccgc agctcgtcaa agagccgcgt ccgcagcgcc tgccacgcca
     8761 agtctctgcg cgtttgttct tcgcttggcg agtcccgctg cacttcctgc ttggccctgg
     8821 ccagatccgc ggcaaacagc gccgacacct gcgaatgacc ttgcttggcc gacgccatcg
     8881 cccgcaccac cgaaccggca aagcggggat ggttgtcgcc gtaataggac accaccttgc
     8941 cgtcgcgcag gtacgacgtg aaaaaaccgt tcgcgtcagg aacaagcgca agctgactgt
     9001 ctcgccaatc ggcccggtgc ggcgcaaaaa aaccgccacg tcccaagctc gccgtccccg
     9061 gatgctcttt ttcatacatc gtgttggggc ttgtgcccgc cgcgatgcag agcgtccgcg
     9121 ccgccagctc gaccacttcg cccgttccgc tgtagcgctc gccgttttgc gcctggcgtt
     9181 cgaactgcac cgcccgcagc gcaccaaacg catcggtcag cgccactttc gggctcagtt
     9241 tttcgatgta gcgaatgcct tcttccaggc ccttggcgac ttcttcgtgg ttgagcctgt
     9301 acgcgggcga atcgagcagg ctcttgcgat agaccagcgt cacgccgcca aagcgcgcaa
     9361 tcagcggcgc aaaatacggc gatcgtcctt gtgctgcggc agcctgtcgc tcggcccgaa
     9421 acgcccgtcc gtgactcagc agctcgtcga ggatttcgcg ttcttcgcga tcaaaacggg
     9481 ccgtcagcgc cgcttcgccc agctcgggca caagcgattc ccagcgatcg agcatttttt
     9541 cgacctgcac cacgtagtac gcggccagct ccgtcgcggt atcaatcgcg gtcagtccgc
     9601 cgccaatcac caccgctggc agccgcacct gcaagttcgc cagggagtcg cgcttgaacg
     9661 ccccggtcag ctgcaggccc atcaggaaat ccgatgcttg ccgaatgccg cgacacaggt
     9721 tgccttccat ttcgaccagc gttggcttgc ccgcgcccgt cgccagcgca acgtgatcaa
     9781 acccgagctg ccacgcatca tcgagttcca gcgtgccgcc aaaccgcacc ccgccatgca
     9841 gctgcaaaag tggttcacgc gagagcgaca gatacagaag ctcgaggaaa tttttgtccc
     9901 agcgcaccgt gatgccgtat tccgacaccc cgccaaaccc cgtcagaacc cgcgcatcaa
     9961 gcggcgcttt caaccgggaa aaatcgcgca ccggcttggg cacaacgcca cttgccaggt
    10021 cgcccgcaag cgctcgatcg atcggttcca gcttcagccc atcgaccgca cagaccgcaa
    10081 acccttcgcg cagaaggtag tggcacagcg tgtagcccgc cggcccgagc ccgacgacca
    10141 gcactttgcg accgttgtac gcaagcggat acggacgggt gcggtgcagc ggattccagc
    10201 gcagaagcag cccgtacaac tcgacgcccc acggcagccc cagcacatcc gtcagcaccg
    10261 acgtttcgac ttccgggatg ttgaccgggt cctgcttttg gaaaatgcac gctttcatgc
    10321 agtcgttgca gattcgatgt cccgtcccgg cacacatcgg gttgtcgagc atgaccagcg
    10381 ccagcgcgcc gagcggatcg ccttgccgac gcagcgtgtg catttccgaa atcttttccc
    10441 cgagcggaca gccggtcagc ggcgccccga gcgggttttg cttcaccgcg ccgtttttgt
    10501 cgcgcatgcc tttgctgcac gagtctttgc tgcgctcgtg gcagtacatg cagtagtcga
    10561 cttcggccaa cacctgcagc cggctcttgc gcggatcggt cagtgcaaag ccgtcccgtc
    10621 ggcgctggtg ttcttcgtgt ccgcacagcg cttccgggat gttcggatcg ggccgatgca
    10681 ggggcacaag ctgcgagaag tcggtcgctt tcggcaggcg aaatcccagg tgtcccgcat
    10741 cgagcagcgc ttgtttttgc gccgccagcc agcgcaccaa gatcgcaagc tccgccgcct
    10801 ggtcatcatc gagcagccgc agcccgagcc gcgccagatc ccgttcactg ggcagtgacg
    10861 aaagaccgtg ccgcgaaagc agcgcttccc cttcgaccgt cgcgctttcg gcctggtccg
    10921 cgctcagtcg gagcttgtcc gcctggcgtt tgatgatctc gtctttgtag cgaaacagcg
    10981 ccagatccgt cgcggtttcg tcccgcagtg ccgcaagcgg cgtcgcaatg tcgggaacaa
    11041 gctgcgccaa aaaccgcgac accgtggccg caagctcaag caaaagcgcc gacagatcct
    11101 tgtccgacag cggctcgcct ttttggtacg catcgtagcg gtcgagcagc gccggatgtt
    11161 cgcgtccgac ctgcgcccgc cagcagtcat gcagccgccg caggccgtaa ggagaaaaca
    11221 gatccgcata cacaaagcca tcgatcccca agacaagcga gtccggggcc tgggttgcgt
    11281 gtgacgtcat cgtccgccta cttgtttttt cgggtcacgg tcagtcgctg gatcacgacg
    11341 ggcactttgg gcgcgtcgtt cgaataggtt tccagccggc tcacttggtc gagcagcggc
    11401 aagttgtcgc actgaccaaa gatggtgtgc tgtccagaaa gccacggcgc ggctttgacg
    11461 gtgatgaaaa actgcgaacc agcggtgttc ggtccgcggc tggccatcgc cagcaccccg
    11521 ccttggtcaa agcgcagctc ggggcgaact tcatcgggca gggcaaaacc gggatcgcca
    11581 ctgccggggg tgaacatgca ctgcgcatcg ccaaagcact tgggatcgcc gctttgaatc
    11641 agaaattcgc cgatcacgcg atgaaccggc aggccgtcgt aaaaaggctt tttctgccag
    11701 cctgtgcgct gcggatgcgg gtctttgaat gcgaccaggc cccgcgcaag cccgacaaac
    11761 gccgcgaccg cttgcggcgc tttgtccgcg aacagatcac agtgcactgc gccgagcggc
    11821 ttgccttcgc tcacaagctc cagcgtggct tgcagtgcgc cctggccttt gattccgacc
    11881 aggctctgtt cgagcacgag cggcggcgtc gttgggccaa ccggcggggg cggcgtggcg
    11941 gcttgggctg caagcagcgt cggcagtgcg agcgcagcga acgtcgagat catcacttcc
    12001 cctctaagca gtggcccatc tacttggcgc cgtcgacatg cagaaattcc gctcgtgatc
    12061 caacggtgat gccatgcttg cgcgcttcgc cgccggcgac ttcgacgacg tactgcgccg
    12121 gctgatcggg gccgcggctc gtttcatcgt gcggaatggt gttttcttcg atgcccacca
    12181 cgatcttgcg ctcgtttagg aaaatcatgt cgagcgggat cagcgtgttg cgcatccaaa
    12241 agcgacgaat ctccggtgcc tcgaacagaa acagcatgcc gctgctcggt gccagctctc
    12301 gccggaacat caggccgcgc gtccgttcct ggtcgttttt cgccagctcc accgtcaccg
    12361 gccatggcgg attgcccgac gacggataaa acgcgaccgt cggtttttgg gctgggcttt
    12421 ggctgactgg ctgcggcgtc ggtggcttgc tgcaaccaag ccaagtcagc gaacttgcgg
    12481 ccagcacaag cgccggcaaa gtcacacgag acgaacggaa ggctggcatg ggcacttacg
    12541 gtttggcggc gggggcgggc tttgcgggtg gtggtggcgt cgtcggggtc tgcgctttgg
    12601 tcgcccgcag cgccgcttgc accattcgct tggcggccac agggtcggtg ttggcgtcga
    12661 tgcccttctg cgcgttttcc cgcatcaggt tcgccaggta gccgagccga atctgcgtca
    12721 cttccgggca gcgtggacct tgtgattcac acaccgccag accggcttcc gcttctgcaa
    12781 gtcccaggtc gaaacgcagc accgttgcat atgccgtcgc cagcacctga tgcacttggt
    12841 agctgtcggc cttgccatac ggacgcagcg tcgcaaccgc ccacgccggc tgctcgatgc
    12901 gcaggtacgc acgcgccagc tcgacggcgg tttcgacgtc atcttgcttg cgatacagcg
    12961 catcttcgag caccgcgatg cggtcgatgt cgcttttgtc ccccgcaaaa ccgggcgtaa
    13021 acaccaccgt cggctgcggc aaaagcagaa gaaacgcaac aatcgcgaga gcaaacaggt
    13081 cgacaagcct tgccacgagg agccactcct tgtcgtcaat ctatcgctcg ctgtgcgctc
    13141 tcgtcaaatc ccgcttcgtg agattgcgcg tcttgtcgaa gctggcgtta cactcctttt
    13201 catgtctgtg ctttgccgga tgcccaagcg gtcgcgttct gtcgggcgcg cgcttttgct
    13261 cggactgctg ctgccgcttc tgccatcgcc cgtcgcgctg gccgatgact ggcaggtgac
    13321 gcgctcggaa tttgacccgc gcatcgtcaa cgaactcaaa gatcaggtgc gcaaaagtcc
    13381 cgatgacagc ggtctgtttc gccggctggt cgggctgtat cgccggcaca gctctctgga
    13441 aaagctgcaa gccgagctgg ccgatcgcgc gcagcgatcc gggcaggcga cggatctgta
    13501 tctggtggcg caggtcgccc gcgaacgggg tcagccaggt gacgccgcga agctgctgga
    13561 ggaggcggtc caacaagggc tcgatgaagc caaagcggcg cttttgttgt ccgagctggc
    13621 gcagaaaaag acgccgcccg atgttgccga agcacgcaaa cagctcgggc gggcgctttc
    13681 cctgttgcgg cccagcgatg cgcgccgacg gcctgcgctg cggcgccagg tggatctgtc
    13741 gctgcaagcg ggggacaccg ccagggccga agccgagctg cgcgatctga tgaagagcgc
    13801 gacggggaca gagtcgaccc ggcttgcgca agagctggcc gagctgctgg cccgcgcagg
    13861 caagtccaag gaatcgctgt ccgaatatcg cgcgctcgca gaaaaacaag caggcgagtc
    13921 gaaagtgcag tcgctgctcc gctgtggcga gctggaagag tcgctgcgcg atgacaatgc
    13981 agccattgca acgtatcgtc aggcgcagaa gctgctgccg gctgggcatc acctccagcg
    14041 cgatgtcagc gagcacctga tcgcgctgtc ccgcaaacgc gacgagctgg tgccgtttat
    14101 caagcagttt gaagccgacc atccgcaggc gcagcgcagc ttttccgatt gggagctact
    14161 cggacggctg tacgacgagc ggggtgacac ggcggcggcg cttttggcct atcgcggtgc
    14221 gctgcgcaaa gatccgcatc acatcgatgt ccggcggcgg ttgattgcga tcctcgagcg
    14281 cggcggtgtg acggcagagg tgttgtcgga atacgagcag ttgattgcgc aagcaccggg
    14341 cgattcccgc gggtatctgg agctggcgga acggcagtgg aaagtcgggc agaaacccaa
    14401 agcgctcctg acactgcgac gagcggcggc ccgttttgcg gctgatccct ctttgcactc
    14461 ggcgctcgcg gagctgtact cgcgctttgg ggaagccgac ctggcgctgc aagagtccga
    14521 gcttttggtc cggctggagc cgcgcgaaga gagctacatc atcaacctgg gcgagctgta
    14581 ctgggcgcgc ggtcgcaaag agcgcgccga cgaggtgtgg aagcgactgc tgacgatggg
    14641 aaccagccga gcgcaaggtc aggcgcggct cgcggatgtc tacgccgagc acaacctgat
    14701 gtcgcaagcg ctcgacctgt accaaaaagc ggtgtcggcg gagccaaaca acctgcagct
    14761 gcggcgcggt ctggcccaag cctacgagcg gctttcgcgc gggcgcgacg cgatggtgac
    14821 gtgggaacag atttattttg ccgcgaaaag tccgtccgag cgactgctcc agatcgaagc
    14881 ccgcaagcag ctcgcgtcgc tggtcggcaa agaatcgcgg ctcagtgcgc agctttatac
    14941 ctggcagcgg cggctggcgg cgcagcttgg ccagcagacc cagacgctgc aaccggatac
    15001 gctgatgctc gggctcttgg tcgccgaaac ccagcagcag gccggcaagc tcggggatgc
    15061 cgagcaagtc ttaagtcagc ttcgcgtccg gttgcccgat gggccgcagc ttgccgaagt
    15121 gctgctatcg ctttgtcagg tgctcaaagc gcagcgcaag ctggaagaag cgatagccgt
    15181 gctcaaacaa gccgcgtcgc ttctgcccga ccgccagcgt gagctgtacg cccagcttgc
    15241 cgatctgtcg atgcagagct accgcgatgg cgatgcgatt cgctatgccg aacaggcggt
    15301 tgccgatgcg agcggcgagc tgtcgctggg ggaaatcctc gaacgacgcg acgaagtgcc
    15361 gcgcgcgatg gcggcctaca agcgagcgat ggagcgcgac ccgcggctgt ttcgggccta
    15421 tatggcgctg gcgcggctgc acgttcagcg cggcgagctt cacgaagcgg cggcgcttta
    15481 tcgcgacgtg gttcggcgga cgccgcaaga agagctggtg ctggaagccg ggcgcaaagc
    15541 catcgacctg cacgaatacc tcggctcgct gggtgagctg ctcaaagagc tgtcgccgct
    15601 ggcctattcg gtggtgccaa ggccggtcta tcgtcgcctg ctcttgtttc tttacgagcg
    15661 ctacgcggcg ccgctgattg ctctttcgcg cagtggcgat gccggcgcgc aggcggaatt
    15721 ggtccggctg gggcagggcg gtctgaagcc gctgaccgaa accctgaccg aaggcgatac
    15781 gaccgagcag cgcttggcgg tatcgctctt ggccgagctg cgcaacccga gcgccgtgcc
    15841 gtctttgctc aacctggtga tggcgggact gccactgcca gggcaagagc cgcgcattgt
    15901 gctgcacggc tcgacaggat ctggcggtgt gccaggcggt gtaccgggcg gtgtatcggg
    15961 cgttcgtacc ggcgcggaca ttgacttgcg cgtcgatgcc ttgctcgcgg tggcgcgcct
    16021 gtccgatccg cagagcgtgc cgggcctgct ccgcctgacc cgcagccgcg aaaaacaggt
    16081 gcgactggct gcgctgtacg gtctcttgcg gctcgccgac aagctcgatc gggctcagct
    16141 tggccagctt gatccgctgc tggccgatgc ggcgccgggt tgtcgcgcgc ttgccgcact
    16201 gttgtttggc agggtcagcg agctgtccgg acagccgatt gcgcccaaga ttcgctcgca
    16261 gcttgtcgcg gcactggaaa cccggcgggt tcgcaacgac gacgccgatg agctgtttgc
    16321 atcagccgcg gcgcaggcgc tgggccgggc gcgggatcgg gcggcggtgc cgctgctcat
    16381 cgacgtgctg cggctgggca atgacgaagt cgagcggcag gcagcgtggg cgcttgggcg
    16441 aaccggcgat ccgcgtgcgt tgccggcgct tgtgcgggcg gtctttggca aacagccggc
    16501 cctgcggatg accgcggcgg cggcgcttgc cagcctttcg accggcccac agccccagcc
    16561 ggcggatgtg ctgccaagcg aaaagcgggg tcttgatggg ctcgatgtgg cgcgcttttt
    16621 gtcggacatt gccggtcagg gcgcgttagg gatcagcaag ccggcatggc tcgacagtcc
    16681 gggggagctt tcgggggcgc tgcgcgatgc gcttgatgat acgccggcgc tggccctgcg
    16741 gacgctggat gacttgctgg gcagtacatc gggtccgccg caggtgcttc ggctcgggcc
    16801 gctggagacc tggctcccgg cagggacgga cgcaagcggc gtgcgtgagt cggtcgcggc
    16861 ggcgcttttg cccgcgctgc gcaagctggg tctgccggac ggtgtggcgt catcaccgct
    16921 gcttcaaccg gcggcgctgc gtgtgctcgg acaaatcgcc cagactcgct ccccgatcgc
    16981 gccgaaagca gcggcggtgc tgcgggatgt gcttctgtcc cgcgcgtcga cagatgtgct
    17041 gctgctttcg gcgtctctct acgccaaagt tccgccgtcc cagcgcgaag cccagccggt
    17101 gctggatgct ctgcttcagc agccggatcg ctggctgcgt ctccagacgc ttcggctggc
    17161 cgccgagcca gacgcccgcc cgctcttgtc cgcgtccgcc atcgtcaaag ccagcctaga
    17221 ccccgatggc ttcatccgcg aagccgccca acgactcaag taacccagtg ttccacgtgg
    17281 aacactgcca aagtccgccc atccccatgt tccacgtgga acatgggcgc gcttgacccg
    17341 cgggagccgc gtgcgaagcg caggggaact ggcggaaaga gcgggaggcg agggtcgcga
    17401 tggttttggg aagtgaaaaa cgggggaagg cgcgggttgg gacggtttcg ggaagtgaaa
    17461 aacgggggga ggcgcgggtt gggacggttt tgggaagtgg aaaacggggg aaggcgcggg
    17521 ttgggacggt tttgggccga cgggcgggca gggcgtgggc gacgtagtgt gatgtatcgt
    17581 cagtgttggc agcgcgggca gaaataggtg gcgcggcctg cctggacgat gcgcacgatg
    17641 gtgcccgcgc agccgcggcg cttacagggc tcgctttcgc gatcatagac ggcgaacagg
    17701 ttttggaaac cgcccagctc gccggatgcc tgcacgtagt cgcgcagtgt cgagccgccg
    17761 gcattgatgg ctttccgcag gatgacgggg atgcttgcgc agacggcttt gacttcgcgc
    17821 ggcgaaagct cgcaggccag gcgctgcggc aggatgtggg cacggtgcag gatctcgctc
    17881 gcgtagatgt tgccaatccc caccaccagc gcttggtcga gcagcgcgac tttgatgggc
    17941 acccgctttt tcctgagcgc ttgggtcagc cagctcggtg tcagctcatg ggaaagcggc
    18001 tcgatgccca ggtctttgag cagcaggtgc tgggccagct tgtcggtttt ggcgagcgtc
    18061 atcaccccga agcggcgcgg atcgcaaaag ctcaccaggg tgccgctctg ggtgtggaaa
    18121 aggacatggt cgtgcttggc cggcgcgagc gggcgtttgg gatctgccgc tacaaagccg
    18181 tttatgcgcc ccgacatccc caggtgaatc agcaccgcgt caccgctgtc gagcctgcag
    18241 acgatgtatt tggcccgccg atcgatgtca atgacggtcc gaccgcggac tttttgctcg
    18301 aagtctgggg gaatcggcac gcgcagatcg cggcgacgca cgacgacatc ggacagaact
    18361 tgttggcaga gaaagccgcg caaaccgcgg cagacggttt caacttcggg aagctccggc
    18421 acgcgcgaat cataggatgt tgcgtgccga gcgtgggggg cgatcggcgg gttacttgcg
    18481 cgcggtcagg cgacggcgga gtccggcagc aaggagcgcg agcgaggcga gcaggcccat
    18541 cgagccggcg acgtttgaaa ccggagacgt gcccggtgcc actgcgcagc ccggaccgcc
    18601 cgcgacacct ggctggcact gatcaccgat gccatcgccg ttggtgtctt tctgatccgg
    18661 gtttttgtcg gcgaagcagt tgtcgcaggc atcgccgatg gtgtcgccat cggtgtcttt
    18721 ttggtcggcg ttcggggtgt cgatgcagtt gtcgcacgca tccggaatgc cgtctttgtc
    18781 gcgatcctgg cggttgtcgc ccgcgcactt gtcgcaagca tcgccgacgc cgtccccgtc
    18841 ggagtccttc tggtcgccgt ttttcacggt ggggcagttg tcgcacgaat cgggcttgcc
    18901 atcgccatcg gtatcgggct gatccgggtt cggcatgctg gtgaggcagg cgtcgcaagc
    18961 gtcaccgatg ccatcgccgt tcgagtcctt ctgatccggg ttcgggacgt tcgggcagtt
    19021 gtcgcagaca tcgccaatgc ggtcgctgtc gcggtcggcc tggtccaagt tgggccggtc
    19081 cgggcagttg tcgcacgagt tgccaagtcc atcgccatcg cggtccggcg ccagcatgtc
    19141 gctacagctt gcggctttgc cggggcccgg cgcgacgtag tcgtcacaag gccgcttcgg
    19201 cgtaaattgt tcgatcggcg tgcagctgtt gccgttggcg aagtagtcga cgcagtagcc
    19261 ttcgccggga cgcgggatgc cgttgcagtt gacgtccttt tgctgggcca ttgccagccc
    19321 agacgaaagt ccgatggcgg caaacagaag tccggcgaga agggggcggc tcgcggtctt
    19381 tctggaaagc gaaaggccct ggcgaatgtc tcgataggtc atgggtttca agctccttat
    19441 gtgtcttgtg cgtcgcgtta ccgtgagatg agagtaagcg gacacttcgg ggggtccagc
    19501 gtcacgcctt cttgctctgt aatgatgaag tctacgcgac gattctgcgc tcgccgatct
    19561 ggcgagtcat tgggcacgat gggacagcgc aggccaaagc ctgccgatga cagccgccac
    19621 ggctcgatgc cgtggtcgac tagatacacc atcaccgact gggcgcgtcg acgcgacaga
    19681 tccaggttgt actggtcgcg gcctttgttg tcggtgtggc cttcgacgcg catccgcttg
    19741 atgtccggac gcgctttgag cacgcgcacc acttcatcca gcactgggta gcttatcggc
    19801 ttgatggtgt cgcgatcgaa gtcgaagaaa atgcgctcgg acagcaccag cttttgccct
    19861 ttgacttcga cgtgggctgg gcgacgcact tggtctgggc agtcctcgcc ctcgcagctc
    19921 ggcggaagat ccggaggcgg cggaggcggc ggaggcggct cgaccggctt cttcggagga
    19981 ggaggcggcg gcggtggtgg tggcggcgga ggcggcggtg gtggccgctt cacgtaagta
    20041 cccgcgtagc tcatgcccag gtacgcgcgc caatcgggca ccccaacgcc aggactcaga
    20101 cccggcccac cgcccaggaa aaacgtgaag ccgtgatacg ccgctttgag cgccacggtc
    20161 agctcggcgg gcgtattggc cagccgatcg gtcacgacaa acggggtgct gccccaggct
    20221 tcggtgacaa gtgaaatcgg tgcagtcggg ctgcgccgaa gctgtagctg gagaccgaga
    20281 ccaaacaaga agctgtgctg ggtttcggcg ttgtaaaaac gctcggtcga cgaaaagatg
    20341 ctgccgacaa acgccgccac ttgcagccgt cgatagtcca gatgggccaa aagccgcggt
    20401 gcaaacgtcg gcagccgcga tcccatgaag ctgtcggcgt tgccggtcgg caccgtcaaa
    20461 tcgcccgcga tacccagacc aaagccgatc ttttccggat cgtcgttttg gcgatagcga
    20521 tacagcaccg ccttgaccga aaagcgcagg tcttcctggc cggcttgggc aagcccgtct
    20581 ttggcaaact gccgcttcac cgtgcgaccg gacgtcggat ctttcggatc gagaaagcca
    20641 ttttgcggca gctcgccggt ctgccaaaac gtcactggca gcgacagcga cacttgcagc
    20701 cgacgcagga acgacaccga aaccgagatg tccccggtca ggcggttttg caccaagtcc
    20761 gccgccggca ccccgccgag cgtgtacacc agcgggcgtc gcgcgtagtg cacaaacagc
    20821 tgcggcacaa acgtcaggtg cggatcgact tcagccagat cgagcgtcac cagcttttgg
    20881 gtgcccggcg ctggatggaa cttgttgatg tcgatcgggg ctgactcacc aagcgcacaa
    20941 gcgaaacgcg gacaggccat tgccacccac gcgccgagcg cgaagaggac aggtttgctc
    21001 gctgcggaaa gtggcggctt gctcatgacc aagagccctt tgtgcggcct aagtgtgcgc
    21061 aatggttgtg ctcgctgggt aggcgagggc tgcttctact ctgacacaaa aaaaacagcc
    21121 agcctagagc gtgcaggcgg tcgcgcagtg tggccaagcc accgccagaa gcggattgtc
    21181 gacgacaaat ttgcccgcaa ggccgtcttc gtggtagtgg ctccgatatg aatccgtcgc
    21241 agaccgcaga agcttccgca tcgcttcccg aaaagtacga cccgcagagt gtcgagccgc
    21301 gctggcaagc cgagtggcag cgccaaggtg cgttccttgc gcctcccgct gtcgattcaa
    21361 gcggcaagcc agacccgcgg cccaagtatt tcatcatgga gatgttcccg tatccatcgg
    21421 ggcggctcca catggggcat gtgcgcaact actcgatcgg cgacgtgctg gcgcgggcgt
    21481 atcggatgaa aggccaccgc gtgctgtatc cgatgggctg ggatgcgttc ggtctgccgg
    21541 cggaaaatgc cgcgatcaaa gccaaagtcc acccgcgcga ctggacggtg aagaacatcg
    21601 agtcgatgaa gggaccgctc ggtcggcttg gccagtcgta tgactggtcg cacgagctgt
    21661 cgacctgcga gccggaatac ttcatccacg agcagcgaat cttcatcgag atgtggaagc
    21721 gcgggctcgc gtatcgcaaa ggtgccctgg tcaactggtg tacgtcgtgc gcgaccgtgc
    21781 tcgccaacga acaagtcgaa gacgggctgt gctggcgctg tcagtcggtg gtcatccagc
    21841 gggaactgac gcagtggttt ttgcgcatca ccgaatacgc gcagcagatc cttgatgaca
    21901 tcgaacagct ccgcggaaag tggccggaca aagtgctgca catgcaggaa aactggatcg
    21961 ggcggtcaga aggcgcacgg attcgctttc cgcttgtgca gccgatcgcc gatgtcaccg
    22021 agatcgaggt gtttaccacc cggcccgata cgctgtttgg cgcgacgttc atgtcgattg
    22081 ctgccgaaca tccgctggcg ctgcggctgt gcgcaggcac ttcgcgtgaa gccgaagtca
    22141 ccgcgtttgt gaacaaggtg cgcgccgaag acaagacccg tcgcggtgct gatgactacg
    22201 tcaaagaggg ctgctttacc ggcgcttgcg tcgtcaatcc cgcaaacggt cgccagatcc
    22261 cggtctacat cgccaacttt gtgctgatgg actacggcac cggcgcagtc atggcggtcc
    22321 cggcccacga tcagcgcgac ttcgagttcg ccaaaaagta cgcactcccc atcgaagtcg
    22381 tgatccagcc ggacggcaca gcgcttgtcg caagcgagct gtcggcggcg tataccgagc
    22441 ggggtcagct tctgaactcg ggggaatttt cggggcagga caacgaagcg gcgaaaaaga
    22501 acatcaccgc gtggcttgcg caaaaggggc agggcggacc gacggtttcg ttccggctgc
    22561 gcgactggct ggtgtcacgc cagcgctact ggggctgtcc gataccgatg gtcgagtgcc
    22621 cccagcacgg ctaccagcca gtgcccgatg ccgagctgcc ggtgcagctg ccgaccgaca
    22681 tcgagtttgt gccgtcgggt cagtcgccgc tcgcgaccca tcccacctgg aagaacgcca
    22741 cctgcccggt ctgtcaggga ccagctcggc gcgaaaccga caccatggac ggctttatgg
    22801 agtcgtcttg gtactttttg cgctacaccg cgcccacgtt cgcaggcggg attttggatg
    22861 aagccggcaa gacgtttttg ccggtcgacc agtacgtcgg cggcagcgag cacgcgacca
    22921 agcacctgat ctactcgcgg ttttttacca agatgctgtc ggactggggt gtgctgccca
    22981 agaccttgcg cgaaccgttt gtgcggcttt tgacgcaggg catggtgctc aaagacgcct
    23041 atcgctgcgc cgagcacgac tacctgtacc cggaagaagt gcaagacggg cgctgcgcaa
    23101 agtgcggcaa ggatgcccag tacctgcgcc agatgaagat gtccaagacg ttccgcaacg
    23161 tcgtcgagcc gatggcgctg gtggaaaaat acggtgccga caccgcgcgg ctgttctgtc
    23221 tgtttgccgc gccgcccgac agtgtgctcg agtggaacga agccggtgtc gaaggctgta
    23281 gccgcttttt gggccgcatg tatcgcctgg tgcagcgctt tgtgtcgctt ccagaggcgg
    23341 ggggcacgcc acctagcgac agtgcagcgg cggatctgcg gcgggtgacg cacaagacga
    23401 ttcgccgcgt caccaccgac atcttcgagc ggcagcagtt taacaccgcg attgccgcga
    23461 tcatggagct gtcgaacgcc atcaccgact ttgctgcaac ccagacggtg cagtcggcag
    23521 cgctgcgcga agcggtcgaa accgcggtcc agcttctggg cccgatggca ccgcacatct
    23581 gcgaagagct gtggcaggcg ctttcgcaca agacgctgct ggcgctgtcg gcctggccgg
    23641 tgttcgacga agccgcggcc aaagacgaca aagtcacggt ggtcgtccag gtgaacggca
    23701 aaaaacgcgc cgagctgcaa gtggccgccg attccagcga ggacgtgatc cagcaggcgg
    23761 cgctgtccga ccccggtgtc aagcaattca ccgacggcaa aagcatcaag aaatcgattt
    23821 acgtcaaagg ccgcctgctc aatcttgtcg tcgcgtagtc gctcgcagcg ggcgccgttc
    23881 ggtgcgataa tcacggcgcg gggcaaagga ttttttccgt tccccgcgct ggatgtcgct
    23941 tgcgatggac gcccgcacaa tcctgctcat ctcagtcgcg ctgaacgttc cgatcctgat
    24001 gttcatgatt cagggtcggc tgcggcgcgc tgacattccg ggtcagacgg agtggatcgg
    24061 cggcattctg ctggtctggc tggcatcgct gctgatgctt ctgcgcggct cgataccgga
    24121 ctttttttcg atcgcgctcg gcaatgcgtg ctggctgttt tgcttgctgg cgtttacgac
    24181 cggaacctac aagtttctgg aattgccgtc gcggctcccg acttcggtgt acgtcatgtg
    24241 tacggtgtcg gcgatcttga tcgggctgtt cgcctacgtg ccgtcgccat ggccgcgctt
    24301 tcccgccatc aacatccgcg tcgtcgtggc ctcggcggct ggcgctctgc tctgtgtgtc
    24361 ggtgctgctt ggcatgaaaa agaccgcccc gcggctaaag ccctgcgtcg agatcctgca
    24421 gctaccagtc tggggtttcg cgctggtgtc gctgctccgc ctgattgcca cgctggtggc
    24481 acctttccat ggcctaagtc cgctgcaagg cagcgccggt cagtcactgc tggtgtttgg
    24541 tcttttggtg gtcgacttga tggtggcgtg ggcgatggtc tatgccaaca accgtcggct
    24601 cgacattgag cggcgcgaaa aagtggcagc gctggccgac agcgaaacgc acatccgacg
    24661 gttccttgaa gcggcgccgg tcgcgctgtt ttcgatgtgt tccgacgggt cgattttgta
    24721 cgccaacggt cgcttcacca agcagctcgg ctatccgctc gcagaaattc cgacgctcga
    24781 tgcgtttttc gaacacgtct ttcccgacag aggctatcgc gatgcgcagc ggaccaagtg
    24841 gcaagcgctg ctctcgccgg gcgacaaaaa cgagcggacc gagctgcggt tcgaatcggc
    24901 gctgcgctgc aaagacggcc aggaaagaac ctttggcgtg caggcgagcg cgatgggtga
    24961 cgaagtgctg ttgacgctgt ttgacctcag cacgcacaaa gaagccgagc aggcgctgcg
    25021 ttttgccaaa gaagaagccg agcgggcgat gcggagcaag agcctgtttt tggccaacat
    25081 gtcgcacgag ctgcgcacgc cgatgaacgc gatcctcgga ctttcgcagc ttgcgcgcga
    25141 gtgcttggct gacaaaaacg agctgcgcga gacccggcac tttttggccc agattcactc
    25201 gtcggcgggt gcacttctgg gaatcctcaa cgacattctc gacgtgtcga agctggaagc
    25261 cgggcggatg gaactggagc aggtgccgtt cgatctgcat gcggtgatcg atcagcagga
    25321 gcttttgcac ggacagaaag tcaaagagcg cgggctcacg ttttcgatca ccatttcgcc
    25381 agcggtgccg cgctttctgg tcggtgatca gcttcggctg tcgcaaattt tgaccaacct
    25441 tctgtcgaac gcgatcaagt tcaccgaacg tggcagcgta tcgctccaga ttgagccgat
    25501 ggccaccgag cccgcgccgg acaaggacgc accgatgcac ttgcgctttg tcgtggccga
    25561 caccggaatc ggcatgaacg aggcccaagt cgcggcgctt gggcagcctt ttgtacaagc
    25621 cgacgcatcg accacgcgca agttcggcgg caccggcctg ggtctttaca tcagcaagca
    25681 gctggtgggg cttttgggcg gtcgtctcga agtgcaaagc caggtgggcc aaggcagccg
    25741 gtttgtgctg atcgcgccgt ttttgcaggt accggcgtcg cgggttcgtc cgctgccatc
    25801 ggattcggat cgctcggcgc agtttcgacc cctgccgctg cgcccgctgc gcgtgctgat
    25861 cgtcgaagac aatccgataa accagctggt cgcgcagcgc tttttggaaa agctgggcat
    25921 caccaccgaa cttgcccaaa acggcgagca ggcgctcggt cttctgcgcg cccagcgaga
    25981 catcgatctg gtgctgatgg atgtgcagat gccggtgatg gatggactgg aagcgacgca
    26041 gcggattcgc ggcgagctgg ggcttgtcct tttgccgatt gtcgcgatga ctgcgcacgc
    26101 ttttcccgaa gagcgcgagc gctgcttggc cgccggcatg aacgatcacg tcgccaagcc
    26161 gatcagtgcg gcgggtctgg cggaagtgat tgcgcgctgg gtgccggtcg atcctgcgca
    26221 ttaaccgccg ctgggccaca cgctcgcgct atgtttggcg ccgcgccggc aagccgccgg
    26281 cctttcggaa ggtgtgtgga aatgcgcaaa cgcagtggtc ccaaagaagc aacgccgtgt
    26341 ctggtgctgc gggtggtgga ctatggcgat gccgaccgaa tcgtccagct tctgaccgcg
    26401 gcccacggca aggtgtcggc actggcacgc ggcgcgcggt ccagccggcg gcggtttggt
    26461 gcggcgctgt catcgttcgg ttttggtgag gcgctgctcg aagaacggcg cggccaggat
    26521 ctgtggattt tgtcggatct gcatgtcagc cgcggctttc ccaacctgcc gctggagctg
    26581 tcgcgctttg gccacgccgc ctacgcctgc gagctgtgcc tgcacctgtg tccgccgcac
    26641 gtccccgagc ccgaggtgtt gcggctgctt gtgacctatc tcgactattt ggatgggctg
    26701 ccgatcgcag acaagccgtc gccgctggcg ctgcgggtgt ttgaactcca gctcttggcc
    26761 gccgtcggtc ttgcgcttca gctggatgac tgcgcggcgt gtggggccca ggttccgccc
    26821 gcgccgctcg ttccgctcga tgtaaagcgc ggtggtgtgc tctgcgagtt gtgctttctg
    26881 ggtccggtgc cgcttcacct gggaccgcaa gggggtggcc tgccgcagcc gctgcgcgac
    26941 agcctgcgaa tgctcggcgg gctacagctc gatccagagg cgctgtcctc ggtgtcgatc
    27001 gatgtgcaag gccatgccaa ggcgcgcgag ctgctccaat cggtgatcca aaaccacttg
    27061 ggtcacgggc tgcgcgccgt cgagttcatc accaagatga acgccttcca gctcccgtaa
    27121 catttccgcc cgcgtcagac accagccttc gcaaaaaccg gcaggtggaa aggggcgggg
    27181 gttaggcggg ggcggggcgg aagcttgtgg cagccaggta gggcagcgcg agcgccaggg
    27241 tttgtagcca caggttggga tcgcgcggcg cagtggccag caatagggcg acaaacagcg
    27301 tggcggcaat catccgcggt ccggcaatcg caagcggaat gcagtgacca cgccgcctct
    27361 gccaaagcac ggctacaagc gaaagcacac cgagccccgc catcgcagag acggacgctt
    27421 ggcccaggtc gccgcgcagc gcatggcgga aacagatcca ccacgaaccg acaatcagcg
    27481 cgtttgtcac aagcggcgcg agcagcagca gccaaagtcg ccgcgcccgc cacggctggc
    27541 caagcacaag ctcggctccc agcgcgaaat agccccaccc aaggatgccg atgagcggca
    27601 cctgcaagtg tcccggttcg gcccaggacc acagcccggc gcgcaccgcg accacttcca
    27661 tcaggctcgc gtcaaatgtg acgagcgcag cggtgatggc ggcccgcgca agcggcgact
    27721 tcgcatcggg ccacagcgcc tggccaacct gccgcgccga cagaaccacc agcggccaga
    27781 tcagcggcac cagcagcggc acatcgacca agcgggcgtt ccaagccggg ctgtactggt
    27841 aaaagtggta aagcgtgatg cagctttctt cacccagata accagcgacg gcgagggcga
    27901 agtagtcgga caaaagcggc ttccacgggg tgctgcgcgc catcgtcacg agcgtgagcg
    27961 aaacgatgac gacgcagagc gtctgaaaca gcgcgatgga caaaggcggg gtggggcggg
    28021 gcgactagcc ctcgatgtag agcaggattt cgccttcttt ggccgggacg ccttctttgg
    28081 tgacgatctc gacgacggtg ccttcgactt cggattcgac cggcatttcc attttcatcg
    28141 attccatgat caccagggtg tcgccctcat tcacctgggc accgacggtg gtcgtgatct
    28201 tccagatgtt gccggtgatg tgcgctttga cggggtgttt tttcttgctc atgggctgct
    28261 cgtgcctctt tgcgttactt gcccaccatc gtatcaacga catcgcccag cttggcagga
    28321 accgccttga acttgagcgc cgccgagatg ccgtcgacaa aacggtagtt gcccttcttg
    28381 tctttggcga agacgcagcg ctttttggga tcgtcgtttt ggaccgtggt atcgccgttt
    28441 ttgatcacag tgccgtcgcc gtctttgcag gtgtcgacca gcgcgggggt cttttcggga
    28501 tcggacgccc agaaggtttc cagaccatcg ccgtcgacat ccatgtcggg caggtagtgg
    28561 ccatccttgt ccttcttaag ccgcagcact tcggcgagcg ctccgtttgc gaacaccgcg
    28621 tcgaacagcg actgcggcgg cgtcaaaaag cctttgacca tgatcttgtc gagccgcgcc
    28681 aggctcagca cttcgagcac gccaccgagc accgcattgt ccagataggt caggttcccg
    28741 gcgttgcgga acgtcgcttc gacgcgggcg ccagacagtt cgagttcaag ctgctggtcg
    28801 ctgattggca cggtgacgcg gaacaccgac ggaccggcgg tcagcttgtt ggctttgact
    28861 tggccatttt tgaacgtgag gatcggctgg ctctggctgt cgagcgagag cttctgaatg
    28921 tcgatcgaca cgttggcgtc gccgaacgga tcgcactggg cggcgttgtc ggggatgccg
    28981 tcgcagttgt agtcgatgcc atcgcagcgc tcgacgccag ccgagatgct catggcggcc
    29041 ttggcggggt ggcgcagctt ggcgagcggg accattccag tgtccgtcgg gaccatcggt
    29101 gaggtcgcgc gatcgtcgca gtcgccttcc gcaacggtta cgccgtcgcc gtcgagatcc
    29161 atttttgcag aagcgtcgtc cgccggcttt ttctgggtcg ggttgtcggc gagtccgtcg
    29221 cagtcgtcat ccatgcggtt gccggcgact tccggcgcgc ccgcgtggac gtttttgtca
    29281 tagtcgttgc agtcaccctt ggcgacgccg ttgtcggttt cccaggtgtt gtctttgccg
    29341 tctttgtcac ggtctttgag aaactgaccc aggtacatcg cgaacttggt gcacatcgag
    29401 tcgccgctcg tcgagccgaa gaactccatc gggatgacga cgtcgtgttt ttccttgaac
    29461 gtgccttcga gttcgggatt ggcgagcgaa gcaatcgcgc ccagcttgtt gtcggctttg
    29521 ccgtcgccat cgaggtcgag accttgaccc gacgcggcga tttccaggtt gctcacgacc
    29581 aggccgcgcg atcccttttt gaagtccacc ttgtcacccg agcactcggg cttggcttcg
    29641 aacggattga ccttgggcgg gttgtcgccg cccatgttgt cattcgcgtt gggatcgatg
    29701 ccgccgcagc ttagggccag gcccgacgcg cacaggaggg gaaaaaaacg ccgggtaagg
    29761 gaaagctgtt ttttcattgc tgacacctcg tttggggaa
//
LOCUS       DAORGN010000040        29714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_581_length_29714_cov_6.798173, whole genome shotgun sequence.
ACCESSION   DAORGN010000040 DAORGN010000000
VERSION     DAORGN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 29714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29714
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_581_length_29714_cov_6.798173"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKL17_06745"
     CDS             <1..716
                     /locus_tag="PKL17_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNK44460.1"
                     /translation="TEDLGLKLENLSIKDLGRAKRVTVDKDNTTVVDGAGKKADIDAR
                     VKQIRAQIEETSSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEKKARVEDALHAT
                     RAAVEEGIVPGGGVALLRAQSVLDGLKLGEEQMYGVAIIRRAIEEPMRWIAQNGGVEG
                     SIVVNKVRDGKGSFGYNASTDKYEDLLDAGVIDPTKVVRTALQNAASVASLMLTTEAM
                     IAEKAKEKAEEGGHAGHAH"
     gene            complement(816..1754)
                     /locus_tag="PKL17_06750"
     CDS             complement(816..1754)
                     /locus_tag="PKL17_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK44461.1"
                     /translation="MANILITGIAGGLSQRVAAKLLADGNSVVGIDYREIRTPLPPSL
                     AKLKVHRAHYNKTIIEDVFRHNSFDAVMHLGRAFSLKESVGKRFDLNVVGSQKIMNLA
                     VQHNVPSLVVLSTFHIYGAHPSNHIPIAEDEPLRAGVQFPQIADAIQMDNMASTWVYR
                     HPQVKTCVLRSTNIIGPTLHNTLTQFLRLRHVPYLLGFNPMMQFLHEDDLADAIIAAW
                     RGKVVGVYNVTSDETVPWRTAISLTRAQTFPILAPVADLFLRATGNFPGYLIDFFRYP
                     CVISDRQFRSKVTWFPRHGLEETLWSAVSSARSSQP"
     gene            complement(1766..2701)
                     /locus_tag="PKL17_06755"
     CDS             complement(1766..2701)
                     /locus_tag="PKL17_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK44462.1"
                     /translation="MLKSTFDSVAQELPPDLLLKCNEILSRLTNLTQIANEVRHHWVD
                     PTVGAAIDSLPNRVNSQGFDPWGFSPQDAKFYYSLARHVYDYFRPEIFGIENLPKGRV
                     LIVPNHSGQLPFDGIVVAVAALLKASPPRLVRAMAERWVSTLPFVNEMFARSGVVLGD
                     PINCRNLLEDDQAILVFPEGARGSGKTFQHRYRLVDFGRGFMRLALQTNTPIVPVAVI
                     GAEESIISIHDWRGLAKLLHAPYVPISPLLPLLGPLAYFPLPTKFRVYFGEPMYFEGP
                     FDDEDSVIQRKVDAVQDRVQQLINEGLLQRRSVFF"
     gene            2825..3394
                     /locus_tag="PKL17_06760"
     CDS             2825..3394
                     /locus_tag="PKL17_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44463.1"
                     /translation="MKTSTVLARMSSIVRFKLTMVLFLAGLFGSGCQPEAFDVTLTIP
                     EQRQPGGSGMIQTFVPTQWVIVVSLADEAKIRGTNPPKKAFVKDFSFAITSTSRPVGD
                     VDTFDYLNTAEFFLEPTRAGSQLQKLKIADIPSKEGPAGEIYPRLISDLDILPLLNEG
                     ARLTATATGTLPPDDVSYGGKFVVGVQVF"
     gene            complement(3506..4531)
                     /locus_tag="PKL17_06765"
     CDS             complement(3506..4531)
                     /locus_tag="PKL17_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNK44464.1"
                     /translation="MPTAESLQLTSLSVHSTALAGNRYMDPTTRRVEVILPPSYEREP
                     SRRYPVIYVLSSFAGTGYQLLNRSPIAESLDERISRLHAQDPTLPEFITVLPDCYTAL
                     GGSQYVNSPVLGRYQDHLVHEVVPLVDAKLRTLANSAHRAVVGRSSGGIGALWLAMNH
                     PDLFSAVASHAGDGFFRMTTPPELLKFCRRVRRYGGPEGALKTWLDAGKGPRPGELFD
                     VMTIFASGAAYSPDPTLPLGFALPVNWHDATIDEAVFARWLRYDPVEICDEEPYRQAL
                     AKLNLIFIDAGTRDEYYLDMAARKLVSRLRSLSIPVVHEEFDDGHMNTNYRFDRSLPA
                     LARAIAG"
     gene            complement(4576..10596)
                     /locus_tag="PKL17_06770"
     CDS             complement(4576..10596)
                     /locus_tag="PKL17_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44465.1"
                     /translation="MPPADPTSKKPGSDDSLLGDFDFGDEDFNWDKLADVPASTSFNS
                     EDELPPELRKAVVKEPDPQPVQAKANADDSAAAKPVPTAAKSSDVAPIVAPQKSATAE
                     SVVDPLALPPLDGEAAPLPPLGAGGSLLGDFDLDFPVGPAKTPVASEPKTEPKTESKV
                     EPKAEPKAEPKAVSPPSVDTPVISVEPPAAATSADDEDDDYDVQIDAAPASDSKSVPV
                     ADPSMTDLVPDFGLSPATVQTAPAASPLSEPSVELSLPPPPALQTAPAAQEPPKAPTR
                     STDAQPKLELAFAEPLPPPPASDEPLPPAQVAAPIAEMPIAAKSTRDSFVALPPIQPG
                     RSPLPLRNQGTPIVPRRLPLPSVENLPPPPPCAESGAALETASRRVILAQFDAETRSG
                     TDDAGRNARLLLAAAMQAEALHENSEAIDRYRRALELAPNNRTALRALRRLLQIPGPT
                     AQPDEAAMLIEREQKQSSQNEQLGLLFQRMELLRAAGQLGDVKALAQDFLSSISRGSH
                     KLGQSIALLAQSDVTLAQGTPTELAPTLDGILAQPMLAAVLRPALQVQRARLDEPAGR
                     DQATAQRFETTFGQSPTCSAALGWLRTAARLPRLRKEEPSPLAKAVRAVQSAALPSAL
                     KASLLRLAARQSPAAEQKAALADAAQLGDALAWDEIACSAEHAQDFATAASSYAKAAE
                     TRRDPWLRSEALRLSARAYLQAGQDEAAQRALVDALSAAAMTDGAHDGVSYLLLERVC
                     RKLGRPSDLLKLWRKQGESSDGQGVYAFLYAADLQLRSAEGNAGREAAIAELQAALKV
                     RPDFLEAALRLYELQSESGQPAEAVKSLLSATQQVQSDPIAELCRRLLREEAAAQLGR
                     LGQHAEAAKVLLSDAVVQDAVTDVAPTLSPARSWQLALLATRLRGMAEPNLLQQLSEV
                     LHQAAGTAQGSLAAQLWFARGNLLSSIQPDPSPEGGAVEESYRQALLAEPGHAAALQR
                     LEMRAALTDRTKLSKSPLSHFVTQGLRARMDLAKGQPSQPLWGIRLLLAEELDAGEPA
                     AALRTYRELRKLESAPNGLLGLDDWTYVTAWRAGQGLEQLERELAQDPDSDTRYALLI
                     LAGEQLESQGKAKLATERYQQALELRPGHPVAKAKLTQALLSAGMHDELARFTAAELK
                     DAPDIQTRVQAFERQALLASLQLSDPMARSEAIENAYRNVLTVDASNHLAMRLLERHF
                     IAHGQWGELIHLYEQMGLTATDTPFAVHISSDRARLRQRILWQQGGDAEATHNQMEND
                     YRLALYRDRTFRPALRLLHEGAVLRDDQAQIAELSQWIGELSIAPSPGEQSQKRSAAV
                     FLTRAAEAASHSGGDADQVIALYQSAISKAADHLPTLRGLLHYALLHQRYQVALDCAE
                     QIAELLYDTEERYLHYMLVGVLAQNLVPDAVRARRALRKGLVLLPDREEAFERLRVSY
                     STTSAQSAEDARALADLIKERLQRSDLSQAQIASLRVDLGQLYAGQLKNRAMAKYELG
                     EALRLKADQPAALYMLGKLHADDSEWQHAIDPLRRYGQMETRPSQLLALHLLLGEIFS
                     EQLHDPQQAIAEYAKAIQLQPTNQTALTKLADLFLAQNRSVETLPLLKRLVKYTEDKQ
                     RKIGYYHRIAGLSELQGDSRGALEALRQAVEVDPVDLSAIRELALFYERTSDVTSLRF
                     HLEAAAGRFRPQIVSKPRDLAVHQALLQILILRRSEQAHLAAGAITSLGGVIPKDLTP
                     LLEKLPRKEATKNGLRDPSTDDTLYPAVVSPSIRMVFRLLAEPLGKLYVSDGRKLQAL
                     GVDRKERLPKSGHPVRDLANKLAADLGIGEFDIYLTAAQGKDDAGQTVPHYTIEPLEP
                     PALIISNVLIDGASEAERRFVLGGLLKILQNQLHLPLRMSSEDLAVLFGGLVRQQVKD
                     YVPVGFAEKRIEAEAQRQRRAIPSKLAVQIVPLAMEFYAAVPNYDGIAKALLLSSHFF
                     GLLWCGSLPAAVASLKRRGPAGEKLIDELYRFALSREADELLRQLSGS"
     gene            complement(10601..16102)
                     /locus_tag="PKL17_06775"
     CDS             complement(10601..16102)
                     /locus_tag="PKL17_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44466.1"
                     /translation="MSSTDNPADLLSNLRPTGRAKDASPPSEAAEAVIAALDDADVSL
                     DTDDESVTLLPDSGSDAIEILDAEPMAAGEAPLAGAIDPVDLIQRRAKNERPRDRIAA
                     LERQLRDEKDHGRAAVLQHEIAVVLERTAQADTDAAQGYAAALQHDPALRPNLWALRR
                     IYYRRGMWPSLLRLLDLEADSAASPRERAELLTEKGHILEDRLGNVEEALTCYRTAHE
                     LDQDALAPIVALEKVLTRQAGESALASTSTRPSDELLSVYRSLLAATKDPGRRVALLI
                     EQARIEEIPRISTDGKDVPKPDVTAALNYLHDAYDVGVDQLRVIDEIVRITAAAGRIP
                     DCLTALEIRAEILEMQAENATQQRKQLLNDQIVGIRRWQANLARERIGNLELAWQYLE
                     KAQEKSPADPLLGPELLTVADAQGQPERLIQQLLERERELRIQRPEDAPPPVGVWLRQ
                     ALALRGMGNDEEADRIEAKVAEVAPSHLLLEMLRRRRAMRNYDLSSLATQLVAEAERA
                     AQGIVGKDGVVVRDPLWAVDALIAATDCALQAGDLATAETLAQRAEALLPNPMTEAGR
                     VPRRLLDAQLEEIYLRSSREGLLAGLFEGRIADGQITQTEEVLLRETLIELYSGILGS
                     PENATPHKRALERLGQTDLRIQRREVLKARQRGNLAEEEAALAQWIQAVSAKAKPPEA
                     DLLRRAELLQKLGRSPEASPIYEKLLEQHPGHPQALEELLRVYLGQGKKDELVQLLQR
                     QIEQVAGKEDDSAVATERTLRARLIDLFEHELNKPDQAAAQYKAMLQRDPQSLPALSA
                     LLHHQRRSGDGTKLAAQLAQLAEHAPTSLLRGDALLRLAELKEEGGGRPQDVDELYEQ
                     ALQALPMPSAQATHAAVGRLRAVMQQRKYDKLADVLEALSDSLSSDDPQTADVVALLR
                     EEQAALLMLSATPTSTAMERADAQLAKASKDLRQTADGKTKTATLVQLASTRVLLNQR
                     REDGKAQGVALTELAELLLSLEDPGAKFVAAELLIRAGIEATLYDDDPGGRIEAARRL
                     LLAYRTLGDQPQVVIPLTDLLSDPTVLEPMAQLPDVVQALRARQTLCPDSEIADRVAF
                     ILLESEAFLIHSSIDEVDDQTAAKLRKSAAEAALRAIQIDPTSIQALLLLRQATAPSD
                     AELDPLRDEPLSPEGSSRLRAYAMYTLRMASLLIDSEARTDLYSEAGQLLLRLSDQDG
                     AAAALRTALDGRPFDSGIFGPLLTLLQRRAEQTSDNGPLLELLDFRLSQVARTDEEKQ
                     HDLPLRIQLLSQRAALHLLTGQNVAAASDLETLLSLSPDHLLSHRRLAALRAHHGDLA
                     AATMHYRRLLDLSQSPTEKLAIHRALGELLQESTPEHAVMHLGEALRIGADLRRESGT
                     AETTDEIEQRVDLHKRLLGLELQKGDRAAAHKTLKTLVSELPTAKELATLRQQVLIEV
                     AGLYERELGDREAALQVLQTLLDEQPLHLAALERIVTLTQASGETARSQGVLNRARDE
                     ARKQAATLADVDTTLSVVPFATLSQVFDWQKQSDARSLAAQATAAVERAQGGSAEMPP
                     PPPAKLPERSVGPPLRSAAFSADARGILLDMWQEVWETGSKLLGPDLTQLSPTPRDRI
                     NAKKVPPAWTTVDTLAQRFGLGNTDLSIAYTLVAGKEKDACVAVGQNLVCGSSFGENL
                     SAWSPQQFFRIVRRLSLLPDRLGVIDCAPAELLLFFAACCQLIGVPTPSLAAAEKSKL
                     DEKVRNLDRAIARKEKNGLRGLSSRMSELAGENGKNLVLGWQRTILRGSAQLAMAITG
                     NLSAALKETGAKLDTDDEHEAKTARALLTFSVSPDLLKLRRELGLTEKE"
     gene            16389..16946
                     /locus_tag="PKL17_06780"
     CDS             16389..16946
                     /locus_tag="PKL17_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNK44467.1"
                     /translation="MSAQVVVTSPSATCHIFTFKEGLLSSVAHDLKLVVDKCVIELTK
                     ADSSDSPDAVTAVSASFDPRSIRVVCAQRDGRDQPGSLSADHRAEIERHIQQDVLHTD
                     RYAEIRFASTSVTKQGSGFAVSGELLLHGQRRTLTVLGTLQDGHFRSEVRLHQPDFGI
                     KPFSAALGTLRVKADLLVSLSVKVS"
     gene            complement(16921..17679)
                     /locus_tag="PKL17_06785"
     CDS             complement(16921..17679)
                     /locus_tag="PKL17_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK44468.1"
                     /translation="MSESGDAPVIEISGLRRHFLLGDELVEALRGVDIRIERGEYVAI
                     VGPSGSGKSTLMNLLGCLDTPTEGRYLLDGVEVQVLDDDALAEIRNRKIGFVFQSFNL
                     LPRASAVDNVALPMLYAGASRAARREAAITALRRVGLGDRLYHRPDQLSGGQRQRVAI
                     ARALVNNPAVLLADEPTGNLDQRTGAEIVALFERLHQEGVTVILVTHDHALAQRTARQ
                     IVIVDGKVARDVRSQTESGAPEPSLSASSPSQTG"
     gene            complement(17676..18872)
                     /locus_tag="PKL17_06790"
     CDS             complement(17676..18872)
                     /locus_tag="PKL17_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK44469.1"
                     /translation="MTSVAKSTRSASWFGRVVLIVSLIVAGVLLRRMWPERSIQVRAA
                     QVSVGKVRDVVSSSTAGEVTPEKHVTLRAELAGQIVAVTRRRGERVSRGELLFRIDPS
                     DLEVKLRQAQAALATADAQVHQAAARVETLKRQSERAKKLLEGGAGTQQVSDDAAAAL
                     QEVNRALMTAHSQRDQASAQLAAAKVARARAEIAAPFDGILTDVHIQLGDTVAPATPM
                     ALLIDDARLHVDATIDEADVAKVKPGQSAELHLDALPDHNITGKVTRTDPAVKRDLKG
                     ARTLTVEVEVSDLVSARAAGLLPGMSANIEILVAENSGVLAVPSNLIVGRGLSRFVYR
                     LVPEGKLHRVHKQPVEVGLSNWERSELLRGVQAGDLLVSSLNEKGLEDGVLVQVASVE
                     ASKESK"
     gene            18874..19359
                     /locus_tag="PKL17_06795"
     CDS             18874..19359
                     /locus_tag="PKL17_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44470.1"
                     /translation="MRAHSSTNNFNRSSTTRRLGGLLLAFCLASCGDGFGPGGSGDPS
                     TQGPGTSKLAIRWTLGGLTLTETRCAQLGIGSMDVYLLRPSDNAVLAVWSSVVCGLDR
                     YSLVELPFGTVKVHVEAIQTPGATMRKCVPYLGETLAPTGPQFNDPPVTVALKAYSSC
                     L"
     gene            complement(19363..20142)
                     /locus_tag="PKL17_06800"
     CDS             complement(19363..20142)
                     /locus_tag="PKL17_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNK44471.1"
                     /translation="MSRTSSRARSPLAVAQSAPTLSTLESAIPACNACPRLRTYCEGV
                     AATKIKRFSDDEYWGRPAPGFGDHEAKVLIVGLAPAAHGANRTGRLFTGDRSGDVLYA
                     GLHREGFASQAHSVARNDGLTLRDVYISVAARCAPPDNRPTPDELSTCRAFLRRELEL
                     LTRLRVVIALGAIAHQAAWLALTSDEKQDHPAPKMPKFGHGREHPIHLSLSSTEPREL
                     TLIDSYHVSQRNTFTGLMTNAMLDDILRRARQLSDGGISTR"
     gene            20290..21906
                     /locus_tag="PKL17_06805"
     CDS             20290..21906
                     /locus_tag="PKL17_06805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HNK44472.1"
                     /translation="MSVVDAAGQYKAFRTEPKRSTKKDMGSSLRRKLFLSALGLSAVL
                     VPTLAVPWFGAGRRKGTRTPSSLPPPYRLPSGFLWGTATAAYQIENTQNDDWAVFEKD
                     VLANHRVEQLAPGQAKPGHIHKLSDFPDEVLAKKTDFDARLEDDLAMAAAMKHNSYRF
                     SISWSRLFPRPDMTDPDPDGIAYYQRLFDTLDRLRLKPAVTLFHFVSPAWFWQEIAGR
                     RGWERPDALEHFERFVAAVVAHFGGRAELWCTLNEPMVYVYAGYLDGVFPPLERRGDP
                     KAIVQVTSQLLRAHALAYRLLKEDAARRGKPVQVGIAQHVRSFQPLRNHHPLDRLTAQ
                     LVEQAFVWDFLDALHSGVLRSKAMNVEQAIPGLVGTQDYVGLNYYGRFYVKSHVTRPT
                     KFDVLMHDPQSDEDRPNELGWASYPEGFRLALLKAHERYHLPLYVLENGTADSKDEDT
                     DRQRLLVEHIRELFLAQKAGADIRGYFHWSLFDNFEWAEGFTARFGLTKIDYQNNFAR
                     TPRPSAALYTRIIEANGLDAELLAQHGPWP"
     gene            complement(22006..22401)
                     /locus_tag="PKL17_06810"
     CDS             complement(22006..22401)
                     /locus_tag="PKL17_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44473.1"
                     /translation="MSGVTFVNPAPDCPTRLCMITPKVADNTNALAKADHRANELAVC
                     TAECNTDADCGSPYENDNRCGKYVCAVASVVPDPTGETFCCKKLCMCDADRQKGFNVD
                     GNGASLPKDSYGVSIPPACQDPATACKLK"
     gene            23048..24742
                     /locus_tag="PKL17_06815"
     CDS             23048..24742
                     /locus_tag="PKL17_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44474.1"
                     /translation="MNRTLRNTVAAVATCLAFAGPAEAKKKQKDADLQPAQPAATPAA
                     QGGGGAVAAPTVESSAPPSKTLERALKLYDSEDYYMASIELNKVVEGQSGDDEGNKQR
                     AEFFMGKTLFNLKYYSASLSYFNKIVDKGQSHRYYQKTLQWLASLSRFLPESAGVLEK
                     IGKYNRQDLEQPALEPVRNELYYLLARYHYTKSNFKEALELFALVPETNEFFGRSKYL
                     EGIIHVRENRGKEAAEAFRQILRKAKAGDANTVEFEDMANLALARVFYATRQFPQAIR
                     FYDKLPSESPDWLPSLFESSWAHFQIDADDKALGNIHTLNAPFFENEFFPESIILRAV
                     IFFQRCNYERAMETLVEFNQTYPALREEILALVKKYPDNAELYKYILKIRSGEAGLSE
                     RVQRASEGALQDRTLAKTLDYVTELDRELSAVDRADPSWKSTAVAGTVLQDLTLQRSI
                     AENEAGGLARKRLERLASEIQELIKQAIKIEYETLQGQKGVLEANIKGEQAAISGTSK
                     KAVNIAADDEHVYWKFNGEYWKDELGYYRFRVVNRCEQARGGAAPAAPAEAGMPPT"
     gene            24782..28531
                     /locus_tag="PKL17_06820"
     CDS             24782..28531
                     /locus_tag="PKL17_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44475.1"
                     /translation="MNLGDPQGRLRHFRPKQEAKTFTERVSGEMFKAPASRILCGPLV
                     AALVLAGVSTAFASGKIQKKEEAVDAKQTALTKPVSKADRDKKKPDLAISDVFSGKGA
                     DLKAVTDAQIKVLQRLIDATGDSDPEKPDLLFRMAELYAEQQSYYNFKARDLDEKIFE
                     AKEKGDTAKENQAKAQQADYEKREKAWLMEAVKKYLEVANGPKYQNYTKMDQVLFYLA
                     YLLTQVKKEDQARVFFKRLIKDYPQSQFLPHAYLSFGEYFFENKDLENALKFYEKVLA
                     YNDSPVYGFALYKKGWVYYNQTDFKSAMATFIDVIDFASKGRPGKPKGDPQLLKEARK
                     DIVRTYAQVGTPEKAWPFFQKYGKDYAPNMLEILADLYNAQGKFLDSIKVYRELIKLN
                     PGSDKLCNYQTEILKNTLSMTGSRAVPETVKELQRLSAIYEKVSEMKNVKKESLDECR
                     DNTAGMLRELATVWHKEAQKTGVMDTYANASDLYKEYIRRFPKEKDSYLMHFWYAELL
                     FKLGSMDNKASSDWYCQAAPVYTDVVKMDTKGKYLKDAAYAAVISWKNCLDVSDEGAD
                     AAEEAKKALAEKRAQRKQAEANKGKDKDKDKGEDQQMAEKPIPEKIQKMMDAFDTYIQ
                     YVPDAPELPTIKYRRARIFFENNYFDKAIPLFKDLAENHSKSELALYSANLLLECIAI
                     KIKLDKAKGDEWVAMMEKQVDKFSGMPELTKDKEFEESLQKIKNSLLRIKIEVVEKLG
                     DYKQAGVLYLQLAERFPNDPKVAELYFNAAVNFERARLIGLAIQARERLIQLRPDDPL
                     AKKAIYRLGQNYQFIAFYEQAADKYETFATKFPGEFADPKEKDPAKKVDAPLALNTAA
                     FFRRGLGQTDKSIELVNLFIKNYGGRKEFFDQAAGVFFDMGQIYEQRKDWAKVKQHFA
                     EYLKSWSAKGGIDRQIIAEVKLGEIAWRESCPLQNQPNGACLEIKRVRASAATALQAK
                     NAAAAKNKKVKNKGPLKLGQCGPETKSKIAVQDRRPAQVKEAMGHFQKALDLYKGGAA
                     ANNVPKNDEQERDRRVAVMTYHAAMAKMYQGDQLYEELIKMTVPTGLVFDPQKKKEME
                     ASTKKFKGWIEGKTKKLLDAQKIYQGVILMKNANWAIAAAARIGQLFQDFANGLFTTE
                     VPTPPPTPSGMERDEWADMFRNAYCDQMTDQAEPLDAKAVEGLKTCLDKSTELNWFNE
                     WSKLCEEELNQIKPSEYPLASEIRAEPGYAGVTLDVAQPFMAPDK"
     gene            28879..>29714
                     /locus_tag="PKL17_06825"
     CDS             28879..>29714
                     /locus_tag="PKL17_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44476.1"
                     /translation="MEKDAESMYKQALSKNPKLAPAMNNLGVMMQRQGRAQEAMLQFE
                     NAIKADPKSPLAVSAYNNRGALLYDRARQANNKAGFDEAVGQLRRALALDAESMPAYQ
                     ILASIYYTTAEADRSKLRLAQLVCDEAKKINPDYAPIYNTLGLIKLRSKDVTGALTEF
                     RKAATLDPGLSEAQLNIGAISLSARSYKQAEEAFQAVIKKVPNHFDATVGMGVALRGQ
                     RKADEAEQWYNKALQLQVDPKMEPRLDAKKCGVLYNLGLLYQDYKNGTPEDMNKGKDY
                     YN"
BASE COUNT         5806 a         8915 c         9211 g         5782 t
ORIGIN      
        1 tcaccgagga tctcggtctg aagctggaaa acctgtcgat caaggatctg ggccgcgcca
       61 agcgcgtcac cgtcgacaag gacaacacca ccgtcgtcga tggcgccggc aagaaggcgg
      121 acatcgacgc tcgcgtcaag cagatccgcg cccaaatcga ggagacctcg agcgattacg
      181 atcgcgagaa gctgcaagag cggctggcga agctcgtcgg tggcgttgcg gtgatcaagg
      241 tcggcgctgc gaccgagacc gagatgaagg aaaagaaggc tcgcgtcgaa gacgctctgc
      301 acgcgacccg cgctgccgtc gaagagggca tcgttccggg tggtggtgtg gcccttctcc
      361 gcgctcagtc ggttctcgac gggctcaagc tcggtgaaga gcagatgtac ggtgtcgcga
      421 tcatccgtcg cgccatcgag gagccgatgc gctggatcgc ccagaacggc ggcgtcgaag
      481 gcagcatcgt ggtaaacaag gtccgcgacg gcaaaggttc gtttggctac aacgcctcga
      541 ccgacaagta cgaggatctc ctcgacgccg gcgtcatcga cccgaccaag gtcgtgcgca
      601 ctgcgcttca gaatgcggcg tcggttgcca gcctgatgct gaccaccgag gcgatgatcg
      661 ctgagaaggc caaagagaag gccgaagaag gcggccacgc cggccacgcg cactagtcaa
      721 aacaaggggt gagcagcccc ttctgacggt gaaaaaccct ccgtaagctt cggcttatgg
      781 ggggttttgt ttttttgggg cgctttcgcg ggcggctagg gctggctgct gcgggcggac
      841 gagacggcgg accacagcgt ttcttccagg ccgtggcggg ggaaccaggt gactttgctg
      901 cggaactggc ggtcagagat gacgcaggga tagcggaaga agtcgatcag gtagccaggg
      961 aagttgccgg tggcgcgcaa aaacagatcc gcaacggggg ccaggatcgg aaacgtctgg
     1021 gcgcgggtca gcgaaatcgc ggtgcgccac gggacggttt catcgctggt gacgttgtat
     1081 acgccgacca ccttcccgcg ccaggccgcg atgattgcgt cggcgaggtc gtcttcatgc
     1141 agaaactgca tcatcgggtt aaagcccagc agatacggca catggcgcag ccgcaaaaac
     1201 tgcgtcagcg tgttgtgcag cgtcgggccg atgatgttgg tcgaccgaag cacgcaggtc
     1261 ttcacttgcg gatgccgata cacccacgtc gacgccatgt tgtccatctg aatggcgtcg
     1321 gcaatttgcg ggaactgcac acccgcgcgc agcggctcgt cttcggcaat cgggatgtga
     1381 ttcgacggat gggcgccgta gatgtggaat gtcgacagca cgaccaggct cggcacgttg
     1441 tgctgcaccg ccaggttcat gattttctgc gaaccgacga cgtttaagtc gaagcgcttg
     1501 ccgacggatt ctttcagact aaacgcgcgt cccaggtgca tcaccgcgtc gaagctgttg
     1561 tgacggaaga cgtcttcgat gatggtcttg ttgtagtggg cgcggtggac tttgagcttt
     1621 gcgagcgacg ggggcagcgg cgtgcggatt tcgcgatagt cgatgccaac gaccgaattt
     1681 ccgtcggcaa ggagcttggc ggcgacgcgc tgggacagac cgcccgcaat accagtgatg
     1741 aggatgttgg ccacggcaag accccctaaa agaacaccga gcggcgctgg agcagcccct
     1801 cgttgatgag ctgctgcacc cggtcttgca ctgcgtcgac cttgcgctgg atcacgctgt
     1861 cttcgtcgtc aaacggcccc tcgaagtaca tcggttcgcc gaagtagacg cgaaacttcg
     1921 tcggcagtgg aaaatatgca agcggcccca aaagtggcaa aagcgggctt atcggcacgt
     1981 agggcgcatg cagcagcttg gcgaggcccc gccagtcgtg gatgctgatg atggattcct
     2041 cggcaccgat cacagcgacg gggacaatcg gcgtgttcgt ctgaagcgcc agccgcataa
     2101 aaccgcgacc gaagtcgaca agccgatagc gatgctgaaa cgtcttgccc gaaccgcgtg
     2161 ctccctcggg aaacaccaga atcgcctggt cgtcttcgag caggttgcga cagtttatcg
     2221 ggtcgcccag caccacgccc gagcgtgcga acatttcgtt cacaaacggc agcgtcgaaa
     2281 cccagcgctc ggccatcgcc cgcaccagcc ggggcggcga cgctttcagg agcgcagcga
     2341 cggcaaccac aatcccatcg aacggaagct gccccgagtg attggggacg atcagcaccc
     2401 ggcctttggg caggttttcg atgccgaaaa tctccgggcg aaagtagtcg tacacatgcc
     2461 gcgccagcga gtagtagaac ttggcgtcct gcggagagaa accccacgga tcgaaaccct
     2521 gcgagtttac ccggttcggc agcgagtcaa tcgcggctcc gaccgtcggg tcgacccagt
     2581 ggtgacggac ctcgtttgcg atctgcgtca agttggtcag tcgagacaaa atctcattgc
     2641 acttgagcaa aaggtccgga ggtagctcct gcgcgactga gtcgaaggtg ctctttagca
     2701 tgtcgacact ctctgcgcgg cggtggcttg gccgcccgag caatgtacca gacctggccg
     2761 ataaacacag cggctcggcc ttcagaatgc gagcacagtc ggaatttctg attacaatgc
     2821 gcgcatgaaa acctcgactg tccttgccag gatgtcttcg attgttcggt tcaagctgac
     2881 aatggtcctc tttttggccg gattgttcgg cagcggttgt caacccgaag cgtttgacgt
     2941 caccctgacc atccccgagc agcgccagcc cggcggttca ggcatgatcc agacgtttgt
     3001 gccgacgcaa tgggtgatcg tggtgagcct ggccgacgaa gccaagatcc gcggtaccaa
     3061 tccgcccaaa aaggcctttg taaaagactt ttcgtttgcg attacgtcaa cgtcgcgccc
     3121 cgtcggtgat gtcgacacgt ttgactacct gaacaccgcg gagtttttcc tggaaccgac
     3181 gcgggccggc agccagcttc agaagctgaa aattgccgac attccgtcga aagaagggcc
     3241 ggcgggggaa atctatccgc ggctcatcag cgacctggac attctgccgc tgcttaacga
     3301 aggggcgcga ctgacggcga cagcgacggg aacgctgcca cctgatgacg tgtcctatgg
     3361 cggcaaattc gtggttggcg tccaagtctt ttagcgaagc aaaccaccca attgctcgat
     3421 atgaagcaga caaaatgctt catattgaaa cccacataag aggcatgtga tacgcctctt
     3481 atcgacgacc gaccgtcccg acaagctatc cggcgatcgc gcgtgccaag gcgggcaggc
     3541 tgcgatcgaa gcggtagttt gtgttcatgt gtccgtcgtc aaattcctcg tggacgaccg
     3601 gaatcgacaa gctgcgcagt cggctgacca gcttgcgcgc cgccatgtcc aggtagtatt
     3661 cgtcgcgggt tcccgcatca atgaagatga ggttcagctt cgcgagcgcc tgtcggtacg
     3721 gttcttcgtc gcaaatttcg acgggatcgt aacggagcca gcgtgcaaac accgcttcgt
     3781 cgatggtggc gtcgtgccag ttgaccggca gggcaaagcc gagtggcagc gtgggatcgg
     3841 ggctgtaggc tgcgccgctc gcaaagatgg tcatcacgtc gaacagctca ccggggcgcg
     3901 gacctttgcc ggcgtcgagc caggtcttta gcgcgccttc gggaccgcca tagcgacgga
     3961 cgcgccgaca gaacttgagc agctcgggcg gggtggtcat gcggaaaaaa ccatccccgg
     4021 cgtggcttgc gacggcggaa aacagatcgg gatggttcat cgccagccac agcgcgccga
     4081 tgccgcccga tgaacgtcca acgacggcac gatgggctga attcgccagt gtgcgcagct
     4141 tggcgtcgac gagcggtacg acttcgtgaa ccaggtggtc ttggtagcgt ccgagcactg
     4201 gcgagttgac gtactgactg ccgccgagcg cggtgtagca gtcgggcagc acggtgataa
     4261 attccggcag cgtcggatct tgggcgtgca ggcggctgat tcgttcgtcg agcgattcgg
     4321 caatcggcga gcgattgagc agctgatagc cggtgccggc aaacgacgaa aggacgtaga
     4381 tcaccggata acgacggctc ggctcgcgct cgtagctcgg cggcaagatc acttcgacac
     4441 ggcgcgtcgt cgggtccata tagcgattgc cggcgagcgc ggtgctgtgg acggaaagcg
     4501 aagtgagctg gaggctttcg gcagtcggca tgccgcgttt atagcaggtc ttgtttggcg
     4561 gcgggtcggg gtcggttagc tgccactgag ctggcgcaga agctcgtcgg cttcgcgact
     4621 gagcgcaaag cggtacagct catcgatgag cttttcgccg gctgggccgc gacgtttgag
     4681 cgacgcgacc gcggcgggca gactgccgca ccacagaagg ccaaagaagt ggctgctgag
     4741 cagaagcgct ttggcgatgc cgtcgtagtt tggcaccgcg gcgtaaaatt ccatcgccag
     4801 cggcacgatc tgcaccgcca gcttgctggg aatggcccgc ctttgacgct gcgcttcggc
     4861 ttcgatccgc ttttcggcaa aaccaaccgg cacgtagtct ttgacctgct ggcgcaccag
     4921 gccaccgaac agaaccgcga ggtcttcact cgacatccgc agcggcaagt gaagctggtt
     4981 ttgcaaaatc ttgagcaggc caccaagcac aaagcgccgc tcggcttctg acgcaccgtc
     5041 gatgagcacg ttcgaaatga tgagcgccgg cggttccagc ggttcgatcg tgtagtgcgg
     5101 caccgtctga ccggcatcgt ctttgccctg tgctgccgtc agatagatgt caaattcccc
     5161 gatgcccagg tccgctgcca gcttgtttgc gaggtcgcgc accggatgac ccgatttggg
     5221 cagccgctct ttgcgatcga cgccgagcgc ttgcagcttc cgtccgtcgc tgacatacag
     5281 ttttccaagt ggctcggcga gaagccggaa caccatccga atgctcggcg agaccaccgc
     5341 cggatacagc gtatcgtccg tcgacggatc gcgcagaccg tttttggtcg cttccttgcg
     5401 cggcagcttt tccaaaaggg gcgtaaggtc tttcggaatg acgccgccca gcgacgtgat
     5461 ggcgcccgcg gccaagtgtg cctgttcact gcgacgcaga atcaggattt gtagcagcgc
     5521 ttggtggacc gcaaggtcgc gcggcttgct gacgatctgc ggacggaaac gaccggcggc
     5581 agcttcgaga tggaagcgca gcgacgtgac atccgaagtc cgttcgtaaa acagcgccag
     5641 ctcacgaatc gccgataggt cgacgggatc gacttcgacg gcctgtcgca gtgcttcgag
     5701 cgcgccgcgg ctgtcgcctt gcagttccga caggcccgcg atgcgatggt agtagccaat
     5761 cttgcgctgc ttgtcctcgg tgtatttgac cagtcgcttg agcagcggca gtgtttcgac
     5821 gctgcgattc tgcgcgagaa acagatcggc cagcttggtc agcgccgtct ggttggtcgg
     5881 ctgaagctga atcgctttgg cgtattcggc gatggcctgc tgcgggtcgt ggagctgctc
     5941 gctgaaaatc tcaccgagca aaagatgcag cgccagaagc tgcgacggac gggtttccat
     6001 ctgtccgtag cgacgcagcg gatcgatcgc gtgctgccac tcgctgtcgt cggcgtgcag
     6061 cttgccgagc atgtacagcg ccgccggctg atcggctttc agacgcagcg cttcgccaag
     6121 ctcgtacttg gccatcgccc ggtttttgag ctgaccggca taaagctggc ccaaatcgac
     6181 gcgcaagctc gcaatctgcg cttgggacag atcgctgcgc tggagccgtt ctttgataag
     6241 gtcggcgagc gcgcgggcgt cctcggcgct ctgggcgctg gtggtgctgt aggacacgcg
     6301 cagtcgctcg aacgcttctt cgcgatcggg caaaagcaca agtcctttgc gcagcgcacg
     6361 acgtgcccgc accgcgtcgg gaaccaggtt ctgcgccagc acaccgacga gcatgtagtg
     6421 cagatagcgt tcttcggtgt cgtacagaag ttccgcaatc tgttcggcac agtcgagcgc
     6481 gacctgatag cgctgatgca aaagcgcata gtgcaaaaga ccgcgcagcg tcggtaggtg
     6541 atccgcggct ttgcttatcg ccgactgata cagcgcaatg acttgatcgg catcgccacc
     6601 gctgtgcgac gcagcctcgg cagcgcgggt caaaaacacc gcggcactgc gtttttggct
     6661 ctgctcgcct ggacttggcg cgatcgacag ctcacctatc cactgcgaca gctctgcaat
     6721 ctgcgcctga tcgtcgcgca gcacggcacc ttcatgcaga agccgcagcg ccgggcggaa
     6781 cgtccgatcg cggtacagcg ccagacggta atcgttttcc atttggttgt gcgtcgcttc
     6841 ggcgtcgcca ccttgctgcc acagaattcg ctggcgcagc cgcgcgcgat ccgagctgat
     6901 gtgcaccgca aacggcgtgt ccgtcgctgt cagccccatc tgttcgtaca ggtgaatcag
     6961 ctcgccccac tgaccatggg caatgaagtg ccgttccaaa agacgcatcg ccaagtggtt
     7021 gctcgcgtcg acggtcagca cattgcgata ggcgttttcg atcgcttccg atcgcgccat
     7081 cggatcggac agttgcaggc tcgccagcag ggcttgtcgc tcaaatgcct gcacgcgggt
     7141 ctgaatgtcg ggcgcgtctt ttagttccgc cgcggtaaag cgtgccagct cgtcgtgcat
     7201 tcctgccgac agcagcgcct gcgtcagctt ggctttggcc actggatgtc cgggtcgcag
     7261 ctccagtgct tgctgatagc gttccgtcgc caacttggcc ttgccttggg attccagctg
     7321 ctcacccgcg agaatcagca gtgcgtagcg cgtgtcggag tcgggatctt gcgccagttc
     7381 gcgttcgagc tgttcgagac cctggcccgc ccgccacgcc gtcacatacg tccagtcatc
     7441 gagacccaaa agaccgttcg gcgcactttc gagcttgcgc aactcgcgat aggttcgcag
     7501 cgccgccgcc ggttcaccag catccagttc ttcggcgagc aaaagccgaa ttccccacag
     7561 tggctgcgac ggctgaccct tggccagatc catccgcgcg cgcagccctt gcgtgacaaa
     7621 gtgcgaaagc ggacttttgc tgagcttggt tcggtccgtc agtgcggcgc gcatttccag
     7681 acgctgcagc gctgctgcgt gacctggctc ggcgagcagc gcttgccggt agctttcctc
     7741 gacggcgcca ccttccgggc tcggatcggg ctgaatcgac gaaagcaagt tgccgcgcgc
     7801 aaaccacagc tgcgccgcca ggctgccctg cgcggtgcct gccgcctgat gaagcacctc
     7861 ggaaagctgc tgcaagaggt tcggttccgc catcccgcgc agtcgggtgg ccaggagcgc
     7921 aagctgccag ctccgggcgg gtgacagcgt cggcgcgaca tccgttacgg cgtcttgcac
     7981 caccgcgtcg ctgagcagca ccttggccgc ttcggcatgc tggccgagcc gtccaagctg
     8041 cgccgcggct tcttcgcgca acagtcgtcg acacagctcg gcaatcggat cgctctgcac
     8101 ctgctgtgtc gccgaaagca ggctctttac cgcttcggct ggctgaccgc tttcgctttg
     8161 cagctcgtaa agccgcagcg ccgcttcgag gaaatcggga cgaaccttca gcgcggcttg
     8221 cagctcggca attgccgcct cgcgtccggc gttgccttcg gcgctgcgca gctgcagatc
     8281 ggcggcatac aggaacgcat acacgccttg tccgtcgctc gattcaccct gtttgcgcca
     8341 caacttcagc agatcgcttg ggcgaccgag cttgcgacag acgcgctcga gcagcagata
     8401 gctgactccg tcgtgggctc cgtcggtcat cgcggctgcg gacagtgcat cgaccagcgc
     8461 gcgttgggcc gcttcgtcct gtccggcctg caaataagcg cgagccgaaa gccgcagcgc
     8521 ttcgctgcgc agccacggat ctcgccgcgt ttctgccgct ttggcatagc tgctcgctgc
     8581 ggtggcaaag tcctgcgcat gttccgcgga acatgcgatt tcgtcccaag ccagcgcatc
     8641 cccaagctgg gctgcatcgg cgagcgcggc tttctgctcg gcggcgggtg actgccttgc
     8701 cgccagtcgc aaaagcgatg ccttgagcgc gctcggcaac gccgcgcttt gcacggcgcg
     8761 caccgctttg gccagcggcg acggttcctc tttgcgcaga cggggcaatc gggccgctgt
     8821 acgcagccag cccagcgcgg cggaacatgt tggactctgt ccaaatgtcg tttcgaaacg
     8881 ctgcgctgtc gcttgatcac gaccggctgg ttcatcaagc cgcgcacgct ggacctggag
     8941 cgccggacga agcaccgcgg ccagcatcgg ctgggccaag attccatcca gcgtcggggc
     9001 cagctcggtt ggcgtgccct gggccagcgt cacatcgctc tgggccaaca gcgcgatgct
     9061 ctgacccagc ttgtgactgc cgcgcgaaat gctcgacagg aagtcctgcg ccagcgcttt
     9121 gacgtcgcca agctgcccgg cggcccgcaa aagttccatg cgctgaaaca gcagaccaag
     9181 ctgttcgttt tggctgctct gtttttgctc gcgctcgatc agcatcgccg cttcatccgg
     9241 ttgtgccgtc ggccctggga tttgcagcaa gcggcggagc gcccgcagcg cggtccgatt
     9301 gttgggcgcg agctcgagag cgcgccgata gcggtcgatt gcttcgctgt tttcgtgcag
     9361 cgcttccgct tgcatggccg cggcgagcag caaacgcgca ttgcggcccg catcgtcagt
     9421 tccactgcgg gtttcggcat caaactgcgc caaaatcacc cgtcgagacg ccgtctcaag
     9481 cgctgcgccg ctttccgcac acggcggtgg cggaggcagg ttttcgacgc tcggcagcgg
     9541 cagtcgtcgc ggcacaatcg gggttccctg gttgcgcagc ggcaggggcg accggcccgg
     9601 ctggatcggt ggcagtgcaa cgaagctgtc gcgcgtcgac ttggccgcaa tgggcatttc
     9661 ggcgatcggc gctgcgactt gggctggcgg aagtggctcg tcgctggctg gcggcggcgg
     9721 cagtggttca gcaaaagcga gttcgagctt gggctgagca tccgtcgatc gggtcggtgc
     9781 tttgggcggc tcttgggcgg cgggcgccgt ttggagcgcc ggtggcggcg gcagcgacag
     9841 ctcgaccgat ggttcgctga gcggacttgc tgctggggcc gtttggaccg tcgctgggct
     9901 gaggccaaag tccggcacca gatcggtcat cgacggatcg gcgacaggga ccgacttgct
     9961 atcgctggcg ggagccgcat cgatctggac atcgtagtca tcgtcttcgt cgtcagcgct
    10021 cgtcgcggcg gctggcggtt cgacggaaat gacaggtgtg tcgacggatg gcgggctcac
    10081 ggccttgggt tcggccttgg gctcggcttt gggctcgacc ttggattcgg tcttgggttc
    10141 ggtcttgggc tcgctggcca cgggtgtttt tgccgggccg acggggaagt cgagatcgaa
    10201 atcaccaagc agcgaaccgc ccgcgccaag tgggggcagc ggtgcggctt ctccatcgag
    10261 cggaggcaaa gccagtggat cgacgacgct ttctgcggtc gcactctttt ggggcgccac
    10321 aatcggcgcg acatccgacg acttggctgc ggtcgggacg ggctttgcgg cggcgctgtc
    10381 gtccgcgttg gccttcgctt gcacgggctg cggatcgggc tcttttacaa ccgccttgcg
    10441 cagctccggg ggcagctcgt cctcggaatt gaaagacgtg ctcgccggaa catcagcgag
    10501 cttgtcccag ttaaagtcct cgtcgccaaa gtcgaaatcg ccaagcagcg aatcgtccga
    10561 ccctggcttc ttgctggttg gatcagccgg aggcacgtct ctactccttc tccgtcagac
    10621 cgagttcgcg acggagcttc aacagatcgg gcgacacgct gaatgtgagc agtgcgcgcg
    10681 ctgtcttcgc ttcgtgttcg tcgtcggtat cgagcttggc gccagtctct ttgagcgccg
    10741 ccgaaaggtt gccggtgatg gccatcgcaa gctgtgcact gccccgcagg attgtgcgct
    10801 gccagcccag caccagattt ttgccgtttt cgcccgccag ttccgacatc cgcgacgaca
    10861 gcccgcgcag accgtttttt tctttgcggg cgatcgcgcg gtcgaggttg cggacttttt
    10921 cgtcgagctt gctcttttct gccgcggcca ggctgggcgt cggtacaccg atgagctgac
    10981 agcaagcggc aaagaacagc agcagttcag ccggtgcgca gtcgatcaca cccaggcggt
    11041 ccggcagaag cgacagccgg cgcacgatgc ggaaaaactg ctgcggcgac caagcgctca
    11101 ggttttcacc aaagctgctg ccgcagacca ggttttgccc gacggccacg caggcgtcct
    11161 tttctttgcc agcgacgaga gtatacgcaa tgctcaggtc ggtgttgcca agcccaaagc
    11221 gctgcgccag cgtatcgacc gtcgtccagg ccggtggcac ttttttggca tttatgcgat
    11281 cccgcggcgt cggtgacagc tgcgtaagat cgggcccgag cagcttgctg cccgtctccc
    11341 acacctcttg ccacatgtcg agcaggatgc cgcgcgcatc ggcggaaaac gcagcactgc
    11401 gcagcggtgg accgaccgac cgctcgggca gtttggccgg cggtggcggt ggcatttcag
    11461 cgctgccacc ttgggcgcgc tcgacggcgg cggtggcttg cgcggccagg ctgcgtgcgt
    11521 cgctttgctt ttgccagtca aacacctgtg acagcgtcgc aaaaggcacg accgaaagcg
    11581 tggtatcgac atcggccagc gtcgctgctt gcttgcgtgc ctcgtcgcgg gctcggttta
    11641 gcacgccttg tgatcgggcc gtttcaccac tggcctgggt cagcgtcaca attcgctcga
    11701 gcgctgccaa gtgcagtggc tgttcatcga gcagcgtctg cagaacctgg agcgccgctt
    11761 cgcgatcgcc gagttcgcgt tcataaagcc ccgcgacttc gataagcact tgttgacgca
    11821 gcgtcgccag ctctttggcc gtcggcagct cactaaccag cgtctttagc gtcttgtgcg
    11881 ccgctgcgcg atcgcccttt tgcaactcca agccgagcag tcgcttgtgt aggtcgacgc
    11941 gctgttcgat ctcatcggtc gtttcagcgg taccactttc gcgtcgcaga tccgctccga
    12001 ttcgcagggc ttcgccaagg tgcatcactg cgtgttccgg cgtgctctct tgcaaaagtt
    12061 cgcccagtgc gcggtgaatc gccagctttt ccgtcggact ttgcgacaag tcgagcagcc
    12121 ttcgatagtg catcgtcgcc gccgccaagt ccccatggtg cgcacgcagc gctgccaacc
    12181 gacggtgtga cagcaaatga tcgggcgaaa gcgaaagcag ggtttccaga tcactcgccg
    12241 cagcgacgtt ctgtcccgtc agcagatgca gggccgcgcg ctgcgacaga agctgaattc
    12301 gcagtggcag atcgtgctgc ttttcttcgt cggtacgcgc cacttgcgac agccgaaaat
    12361 cgagcagttc gagcagcggt ccgttgtcgc tggtctgttc ggcgcgacgc tgaagcaggg
    12421 tcaaaagcgg tccaaaaatg ccgctgtcga aaggccgtcc gtcgagagcc gttcgcagtg
    12481 ccgccgccgc accgtcttgg tcggacagcc gcaaaagcag ttgaccggcc tcgctgtaca
    12541 agtcggtgcg cgcttcgcta tcgatcagca gcgacgccat ccgcagcgta tacatcgcat
    12601 aggcacgcag ccgcgacgat ccttccggcg acagtggttc gtcgcgaagc ggatcgagct
    12661 cggcatcgct gggtgccgtc gcctggcgaa gaagcagcag cgcttggatg ctggttgggt
    12721 caatctggat ggctcgcagc gccgcttctg ccgcggactt tcgcagcttc gccgcggtct
    12781 gatcgtccac ctcgtcgatc gagctgtgaa tcaggaacgc ttccgactcg agcaaaatga
    12841 acgccacccg gtcggcaatt tcgctgtcgg ggcacagcgt ttggcgggcc cgcagcgctt
    12901 gcacgacgtc aggtagctgc gccatcggct caagtaccgt cggatcggac aaaagatcgg
    12961 tcagcggaat caccacctgc ggctgatccc cgagcgtgcg ataggcgagc agcagccttc
    13021 gggccgcttc gattcgtccg ccggggtcat cgtcgtatag cgtcgcttca attccggcgc
    13081 gaatcagcag ctcggcagcg acgaacttgg cgcctggatc ttcgagagac aacaggagct
    13141 cggccagttc cgtcagcgcg acaccctgcg ccttgccatc ttcccgacgc tgattgagca
    13201 gcacgcgcgt gcttgccagt tgcaccagcg ttgcggtctt ggttttcccg tccgcagttt
    13261 gacgcaggtc tttgctcgct ttggccagct gcgcatcggc gcgttccatc gccgtgctcg
    13321 tcggtgtggc ggacagcatc agcagtgccg cttgctcttc acgcaaaagc gcaaccacgt
    13381 ccgccgtctg tggatcgtcg gacgacagcg aatccgacag cgcttccagg acatcggcca
    13441 gcttgtcgta tttgcgctgc tgcatcactg cgcgcagcct gccgacagcc gcgtgcgttg
    13501 cttgggccga cggcatcggc agtgcttgca gcgcctgttc atacagctcg tcgacgtctt
    13561 gcggccttcc gccgccttcc tcttttagct ccgccagtcg aagcagcgcg tcaccgcgga
    13621 gcaggctcgt cggcgcgtgc tcggcgagct gggcgagctg ggccgccagt ttggtgccgt
    13681 cgccgctgcg tctttgatgg tgcaaaagcg ccgatagcgc cggcaggctc tgcggatcgc
    13741 gctgaagcat cgctttgtac tgcgccgccg cctgatcggg cttgttgagc tcgtgctcga
    13801 acaggtcgat aagccgcgcc cgcagcgtgc gttcggtcgc gaccgccgag tcgtcctctt
    13861 tgccggcgac ctgctcgatt tggcgttgca gaagctggac aagctcgtcc tttttgccct
    13921 gcccaaggta cacccgcaga agctcctcca gcgcctgcgg atgccccgga tgctgttcca
    13981 gcagcttttc gtaaatcggg cttgcttcgg ggctgcgacc cagtttttgc aaaagctcag
    14041 cccggcgcag aagatccgct tccgggggct tggctttggc gctgactgct tgaatccact
    14101 gggccagggc cgcttcttct tccgccaggt tgccgcgctg ccgggctttg agcacttcgc
    14161 gtcgctgaat ccgcagatcc gtctgtccca agcgctcaag cgctcgtttg tgcggcgtcg
    14221 cattttcggg cgaaccaagg atcccggaat aaagctcgat gagtgtctcg cgcagcagaa
    14281 cttcttctgt ctgcgtaatc tgtccgtctg cgatccggcc ttcaaacaga cccgccagca
    14341 gtccttcgcg gctgctgcgc agataaattt cttcaagctg cgcgtcgagc agtcgtcgag
    14401 gaacccgccc cgcttccgtc atcggattgg gcagaagtgc ttcggctcgt tgcgccagcg
    14461 tttcggcggt ggccagatcc cccgcttgca acgcgcagtc ggtcgccgca atcagcgcat
    14521 cgaccgccca aagcggatcg cggacgacga cgccgtcttt gccgacgatg ccttgcgcgg
    14581 cgcgctcagc ttctgcgaca agctgggtcg ccaggctcga caagtcgtag tttcgcatcg
    14641 cccgacgacg acgcagcatc tcgagcagaa gatggctcgg tgccacctcg gcgactttgg
    14701 cctcgatgcg gtcggcttcc tcgtcgttgc ccattccgcg cagtgccagc gcttgtcgaa
    14761 gccatacccc cactggcggc ggtgcgtcct cgggtcgctg gatgcgcagc tcccgctcgc
    14821 gttcgagaag ctgctgaatc aatcgttccg gctgaccttg cgcatctgcg acggtcagaa
    14881 gctctggtcc cagcagcggg tccgccggtg acttttcctg cgctttttcg agatactgcc
    14941 acgccagctc caggttgccg atgcgctcgc gtgcaagatt ggcctgccag cgacggatgc
    15001 caacgatctg atcgttcagt agttgtttcc gctgctgcgt cgcattttcg gcctgcatct
    15061 cgaggatttc ggcgcgaatt tccagtgccg tcagacagtc gggaatccgt cccgctgccg
    15121 cagtgatgcg cacaatttcg tcaatgaccc gcagctggtc gacgccgaca tcgtaggcat
    15181 cgtgcaggta gttgagcgcc gccgtgacat cgggcttggg tacgtctttc ccatcggtag
    15241 agatcctcgg gatctcctcg atgcgggcct gttcgatgag cagcgccacc cgtcggcccg
    15301 gatctttggt tgccgcaagt agtgatcgat acaccgacag cagctcgtcg ctaggacgtg
    15361 tcgatgtgct ggccagcgcc gattcccccg cttgtcgcgt cagcactttt tccagcgcga
    15421 cgatcggggc cagcgcgtct tgatccagct cgtgtgcggt tcgatagcag gtcagcgctt
    15481 cctcgacgtt gcccaggcga tcttcgagaa tgtggccttt ttccgtcaga agctctgccc
    15541 gttctcgcgg gctcgcggcg gaatctgctt ccagatcgag cagccgcagc aaacttggcc
    15601 acattccccg tcggtaataa atgcgacgca gcgcccacag attgggccgt agcgccgggt
    15661 cgtgctggag cgccgccgca tagccttggg ctgcgtcggt atccgcctgc gcagtgcgtt
    15721 cgagcacgac ggcgatttcg tgctgaagca ccgccgctcg accgtggtct ttttcgtcgc
    15781 gcaactgacg ttccagcgcg gcgatgcggt cgcgagggcg ttcatttttg gctcgacgct
    15841 ggatcagatc gaccggatcg atggcacccg ccaggggggc ttcgccggct gccatcggct
    15901 cagcatcgag aatttcgatg gcatcagagc cgctatcggg cagcagcgtc accgattcat
    15961 cgtcggtgtc gagcgaaaca tcggcgtcgt cgagtgctgc gatgaccgct tcggctgctt
    16021 cggatggtgg gcttgcgtct ttggctcgac ccgttggccg caagttgctg agaagatccg
    16081 ctggattatc ggtggaagac atggatgcgc ccgtcgtaac ccacgcccta ggctccctcg
    16141 gtccgaccag gtggacgtag aaggctgctc ggcaccgcgc ctagcgggca agcctagaaa
    16201 gcctggtaat acattgagac tttagtatcg acgcccggat agtgaagtgt caaaccaaag
    16261 cgtttgccga gatttacagc ggacttatct aggtgggtac tgtaggtcaa tgtctgtatg
    16321 tgaaatacag aacaagcgca ttttggcggc tatccaaact ggcaaacaag ctgctaaagt
    16381 gcgccgcaat gagtgcccaa gttgtcgtca caagtccgtc agcaacctgc cacattttca
    16441 cgttcaaaga gggcctgctt tcgagcgtcg cgcacgatct taagctcgtc gttgacaaat
    16501 gtgtgattga gttgacaaaa gctgactcgt ccgattcgcc cgatgcggtg acggcggtgt
    16561 cggcgagctt cgatccgcgc agcattcggg tggtttgtgc gcagcgcgac gggcgtgatc
    16621 agccaggctc gctgtcggca gaccatcggg ccgaaatcga gcggcacatt cagcaagatg
    16681 tgctgcacac cgaccgctat gcggaaatcc gctttgcgtc gaccagcgtg acaaaacaag
    16741 gcagtggttt tgcggtaagc ggcgagcttt tgttgcacgg tcaaaggcgg actctgacgg
    16801 tgctcgggac gctgcaagac gggcatttca gaagtgaagt ccggctgcat caacccgact
    16861 ttggcataaa gccatttagt gctgcgctgg ggacgctgcg ggtcaaagcc gatctgctgg
    16921 tcagcctgtc tgtgaaggtg agctagcgct caacgatggc tcgggggcac ctgattcggt
    16981 ctgggaacga acatcgcgcg cgacttttcc gtcgacaatc acgatctgcc gcgccgtcct
    17041 ttgcgccagc gcgtgatcgt gggtcaccaa aatgaccgtc acgccttctt ggtgcagtct
    17101 ttcaaacagt gcgacgattt ccgccccggt ccgctgatca agatttcccg tcggctcgtc
    17161 cgccaaaagc accgccggat tgttgacgag cgcacgggca atcgccaccc gctgtcgctg
    17221 tccacccgag agctgatcgg gccggtggta cagccggtcg cccagtccga cgcgacgcag
    17281 cgccgtgatc gcagcttccc gtcgtgccgc ccggcttgct cccgcgtaca gcatcggcag
    17341 cgccacattg tcgacggcac ttgcccgcgg cagcaggttg aagctttgaa agacaaaacc
    17401 gatcttgcga ttgcggattt cggccagtgc gtcgtcgtcc agaacctgca cttcgacgcc
    17461 atccagcaga tagcggcctt ccgtcggtgt atcgagacag ccgagcaagt tcatcagcgt
    17521 cgacttgccc gaaccggacg gaccgacgat ggccacgtac tcgccgcgct cgatgcggat
    17581 atcgacgccg cgcagggctt cgaccagttc atcacccagc agaaagtgac gccgaagccc
    17641 gctgatctcg atcaccgggg cgtcgccgct ctcgctcact tgctctcctt gctcgcttca
    17701 acgctggcca cttgcaccag cacgccgtct tcgagccctt tttcattgag cgacgacacc
    17761 agcaagtcac ccgcctgcac gccgcgcaaa agctcactgc gttcccaatt tgacaggccg
    17821 acctcgacgg gctgtttgtg gacccggtgc agcttgcctt cgggaaccaa acgatagaca
    17881 aagcggctga gcccgcgtcc gacgattaga ttcgacggca ccgcgagaac gcccgaattt
    17941 tccgcgacca aaatttcgat gtttgccgac atccccggca aaagtccggc ggcgcgggcg
    18001 ctcaccagat cggatacttc cacttcgacg gtcagcgtgc gtgcaccctt gagatcgcgt
    18061 ttgaccgccg gatcggttcg cgtgactttg cctgtgatgt tgtgatccgg gagcgcgtcg
    18121 aggtggagct ccgcactttg acctggcttg actttggcga catcggcttc gtcgatggtt
    18181 gcgtccacat gcagccgcgc atcgtcgata agcagcgcca ttggggtcgc cggtgcgacg
    18241 gtatcaccaa gctgaatgtg tacatccgtc aagattccgt caaaaggcgc cgcaatctct
    18301 gcccgtgcgc gtgccacttt cgctgccgca agctgcgccg acgcctgatc gcgctggctg
    18361 tgggcggtca tgagcgcgcg attgacctcc tgaagtgctg cggctgcatc gtcgctgacc
    18421 tgctgcgtgc cggcgccgcc ttcgagcagt tttttcgctc gttccgactg ccgcttgagc
    18481 gtttcaaccc gcgccgccgc ctggtgcact tgggcatccg ccgtcgccag cgctgcttgg
    18541 gcttgccgca gcttgacttc tagatcgctc ggatcgatgc gaaacagcag ctccccccgg
    18601 ctgacgcgct cgccccgtcg acgtgtgacc gcgacaatct gtcctgccag ctcggcgcgc
    18661 agcgtcacat gcttttcggg cgtcacttcg cccgccgtcg atgaagacac gacatcacgt
    18721 accttcccga cgctgacttg cgctgcccga acttgaatgg atcgttccgg ccacatgcgc
    18781 cgcagaagca cgccagcgac gatgagtgat acaatgagaa caactcgccc aaaccacgaa
    18841 gcagagcgag tcgacttggc cacggaggtc atgatgcgcg cgcactctag cacgaacaat
    18901 ttcaaccgaa gttccacgac gagacgtctt ggcggtctgc tgctcgcctt ctgtttggca
    18961 agctgcggcg acggctttgg acctggcggc tctggcgatc cgtccaccca agggcctggc
    19021 accagcaagc tcgcgatccg ctggacactc ggtgggctca ctcttacgga aacgcgctgt
    19081 gcgcagcttg gcatcgggtc gatggatgtc tatcttctgc gacccagcga caacgccgtc
    19141 ctcgccgttt ggagcagcgt cgtctgtggg ctcgatcgct attcgctcgt cgagctgccg
    19201 tttggaaccg tcaaagttca cgttgaagcg atccaaaccc caggggcgac gatgcgaaag
    19261 tgcgtgccct atcttggcga aacactggca ccgacgggac cgcagtttaa tgatcccccg
    19321 gtgaccgtcg cgctcaaggc gtacagctcc tgtctgtagt cgtcagcggg tcgatattcc
    19381 gccgtctgaa agttgccggg cccgccgcaa gatgtcatcg agcatcgcat ttgtcataag
    19441 ccccgtaaac gtgttgcgct gcgagacgtg gtagctgtcg atgagcgtca gctcgcgcgg
    19501 ctcggtcgac gacaggctta ggtgaatcgg atgctcgcgt ccgtggccaa acttgggcat
    19561 tttcggcgcg ggatggtctt gtttttcgtc gctcgtcaga gccagccacg ccgcctgatg
    19621 ggcaatcgcc ccaagcgcaa tcaccacccg caagcgggtc agtagctcta gctctcgacg
    19681 gagaaatgcc cggcaggtcg acagctcatc cggtgtgggc cggttgtcag ggggcgcgca
    19741 gcgagcagcg acgctgatgt acacatcgcg aagcgtcaga ccgtcatttc gggcgacgga
    19801 gtgggcctgc gatgcaaagc cttcgcgatg cagtccagcg tacagcacgt cgccgctgcg
    19861 atcgccggta aacagacgac ccgtccggtt tgcgccatgg gctgccgggg ccagcccgac
    19921 gatcagaacc ttcgcctcgt ggtcgccaaa tcctggggcc ggtctgcccc aatactcgtc
    19981 gtctgaaaaa cgcttgatct tcgtcgctgc aacgccttcg cagtaggttc gcaggcgtgg
    20041 gcaggcgttg caggccggaa tcgccgattc cagcgtcgaa agggtagggg cggactgggc
    20101 aaccgcgagc ggactgcgag cccgagacga ggtacgcgac atgtatctca cattatcgca
    20161 tagcttgcgt ttggattgca cacgttcatg ggcacgcata gcgcaaaccc accggaaaag
    20221 cggcggcaaa aatagcgcac gcaagcattt cgattgatgg atagcgacta tgtggatatt
    20281 gcagcgtcga tgtcggttgt ggacgctgct ggacagtaca aggccttccg aaccgagccc
    20341 aagcgcagta caaagaaaga catgggatct tcgctgcgcc gcaagctgtt tctgtcagct
    20401 ctgggtctat ccgcggtact tgttccgacg ctggctgtgc cgtggttcgg tgcgggccgg
    20461 cgcaaaggca cccggacacc gtcgagtctg ccgccgccct atcggttgcc gagcggtttt
    20521 ttgtggggta cagcgacggc agcgtatcag attgaaaaca cccagaacga tgactgggcg
    20581 gtgtttgaaa aagacgtgct ggcaaaccat cgcgtcgagc agctggcccc gggacaggcc
    20641 aagccaggtc acatccacaa gctgtccgat tttcccgacg aggtgctggc caaaaagacc
    20701 gacttcgatg cgcggctcga agacgatctg gcgatggcag cggcgatgaa gcacaactcg
    20761 tatcgctttt cgatttcgtg gagccggctc ttcccgcggc ctgacatgac agatcccgat
    20821 cccgacggaa tcgcttatta ccagcgcctg tttgacacgc tcgatcggct gcggctcaag
    20881 ccggcggtga cgctgtttca ctttgtcagt ccggcgtggt tttggcagga aatcgcgggt
    20941 cgacgcggct gggaacgtcc ggacgcgctg gaacatttcg agcgcttcgt cgctgcggtc
    21001 gtcgcgcatt ttggcggacg cgccgagctg tggtgcacgc taaacgaacc gatggtctat
    21061 gtatacgcgg gctatctcga cggtgtgttt ccgccgctgg aacgccgcgg tgatccgaaa
    21121 gcgatcgtac aagtcacaag ccagctcctg cgggcccacg cgcttgcgta tcgactgctc
    21181 aaagaagatg cggcgcgccg aggaaaaccc gtgcaagtgg ggattgccca gcatgtgcgc
    21241 tcgttccagc cgctgcgcaa tcatcatccg ctggatcgtc tgacggcgca gcttgtcgag
    21301 caggcgtttg tctgggactt tctcgatgcg ctgcacagcg gtgtgcttcg atccaaggcg
    21361 atgaatgtcg agcaggcgat tccggggctt gtcggcacgc aagattacgt cggactgaac
    21421 tactacgggc gcttctacgt gaaaagtcat gtcaccaggc cgacgaagtt cgatgtgctg
    21481 atgcacgatc cgcagtccga cgaggacagg cccaatgagc taggctgggc gtcgtatccc
    21541 gaaggcttcc ggctggcgct tttgaaggcg cacgagcggt atcatctgcc gctctacgtt
    21601 ctcgaaaatg gaacggcgga ctcgaaggac gaagacaccg accgccagcg actgctggtc
    21661 gagcacatcc gcgagctgtt tctggcgcag aaagccggcg cggacattcg tggctatttc
    21721 cactggtcgc ttttcgacaa cttcgagtgg gccgagggct ttacggcgcg ttttgggctg
    21781 acgaaaatcg actaccaaaa caactttgcg cgcacaccac ggccatccgc ggcgctttac
    21841 acccgcatca ttgaggcaaa cgggctcgat gctgaactgc tcgcgcagca cggtccgtgg
    21901 ccgtaaccaa aaacggctgg cttgttcgca aacgtattgg gggaaaaggg gaggggctcg
    21961 ggcctcgtct ccgagagaga ggagacgagt tgtgcgtcgg tcggactact tcagcttgca
    22021 ggccgtcgcc gggtcttggc aagcaggcgg gatcgacacg ccgtaggagt ctttcggaag
    22081 gctggcgccg ttgccgtcga cgttgaagcc cttctggcga tccgcgtcgc acatgcagag
    22141 cttcttgcag cagaacgttt cgccagtcgg atcgggaacc accgacgcaa ccgcacagac
    22201 gtacttgcca cagcggttgt cgttctcata agggctgccg cagtcggcgt cggtgttgca
    22261 ctctgcggtg cagaccgcaa gctcattggc gcggtgatct gccttcgcca gagcattggt
    22321 gttgtccgcc accttcggcg taatcataca cagacgggtc ggacagtcgg gagcaggatt
    22381 cacaaacgta acaccgctca tgaccttcga tgcgagcgga ttgttgcagg ctcggccgat
    22441 ctggttatcg gtgcaccccg atagcatgag ggcgaggccg agggaacaaa acgaaaacca
    22501 cgcggctaga cggcgcttca tacttaatag atcctccgac ctggatgtca caagtcttgg
    22561 acaggataca cggttcacga gcctagtcaa gccttcgtga cccgcctgcg gctcctccca
    22621 cgctcttgta cgatccatcc aaaggctccg gctaagccag gcacccgtac tgcgctggta
    22681 cgctggcgca ttcatgcgct tgtgggtagg atcagatgct gcgttgtcgg ataactccta
    22741 catcgatcgc ggtcctcttt tgctgctggt gcaaaagttg ggtttgcgcc tgccgtggcg
    22801 gccaaaaacc cggtgttttc ctctggctca cccgagcgct cggtaaataa gcgagccttg
    22861 acaccatgaa tttgcggttc gtataatcgc cacgtttggc tacggaagac acaatactgt
    22921 cgatatggcg tgggctaaca aggctcgatg cggtcgacca gatctcgttg gtgagtatgg
    22981 tccactcgtc gtggcatcga acaactatcc gcatgtgccg aagcggtttg gagacagggg
    23041 tttgcgcatg aaccgtactc tgaggaacac cgtcgctgct gtcgcaactt gcctggcgtt
    23101 tgccggcccg gcagaggcga agaagaagca aaaggacgct gacctgcagc ccgcccagcc
    23161 ggccgctact ccggcggcgc agggtggcgg tggcgctgtt gcagccccga cggtggagag
    23221 cagtgcgcct ccatcgaaga cgctggagcg tgcgctcaag ctgtatgaca gcgaagacta
    23281 ctacatggcc tcgatcgagc tgaacaaggt cgtcgaaggt cagtcgggtg acgacgaagg
    23341 taacaaacag cgtgccgagt tcttcatggg caagacgctg tttaacctga agtactactc
    23401 ggcgtcgctg tcgtacttca acaagatcgt cgacaaaggt cagtcgcatc gctactacca
    23461 gaagacgctt cagtggctcg cgtcgctgtc gcgcttcctt cccgagtcgg ctggcgtgct
    23521 cgagaagatc ggtaagtaca accggcagga cctcgagcag cccgctctcg agccggtccg
    23581 taacgagctg tactatctgc tcgcgcggta tcactacacc aagagcaact tcaaagaggc
    23641 gctcgagctg ttcgcgctgg tgcccgagac gaacgagttc tttgggcgct cgaagtacct
    23701 cgaaggcatc atccatgtcc gtgaaaaccg cggcaaggaa gctgccgagg cgttccgtca
    23761 gattctccgc aaagccaaag cgggtgacgc caataccgtc gagttcgaag acatggcgaa
    23821 cctggctctc gctcgcgtgt tctacgcgac gcggcagttc ccgcaggcga tccgtttcta
    23881 cgacaagctg ccgtccgaat cgccggattg gctgccgtcg ctgtttgagt cgagctgggc
    23941 gcacttccag atcgatgcag acgacaaggc gcttggtaac atccacacgc tgaatgctcc
    24001 gttcttcgag aacgagttct tccccgagtc gatcattttg cgcgccgtga ttttcttcca
    24061 gcgctgcaac tatgagcgcg cgatggagac gctggttgag ttcaaccaga cctatccagc
    24121 gctgcgagag gaaatcctcg cgctcgtgaa gaagtacccg gacaacgccg agctgtacaa
    24181 gtacatcctc aagattcggt cgggtgaagc cggtctgtca gagcgcgtcc agcgtgcatc
    24241 ggaaggcgca cttcaggacc gcacactggc caagacgctc gactacgtta ccgagctgga
    24301 tcgcgaactg agcgccgttg atcgcgccga cccgagctgg aagtcgaccg ccgttgctgg
    24361 caccgtgctt caggatctga cgctacagcg ctcgatcgcg gaaaacgaag cgggtggtct
    24421 ggctcgcaag cgtctggagc gactggcgag tgagatccag gagctcatca agcaagcgat
    24481 caagatcgag tacgaaaccc tccaaggtca gaagggcgtg ctcgaggcca acatcaaggg
    24541 cgaacaggcg gccatcagtg gaacctcgaa gaaggcagtg aacatcgctg ccgacgacga
    24601 gcacgtctac tggaagttca acggcgagta ctggaaagac gaactgggtt attaccggtt
    24661 ccgcgtagtc aaccgctgcg agcaagcgag gggcggagcg gctcctgcgg cgccagcgga
    24721 agcaggaatg ccgccgacgt aagtgcgaag tagcggaaga tcccgagtgt agtcgggcgg
    24781 tttgaaccta ggcgacccac aaggtcgcct gcgccatttc cgtcccaagc aagaggcgaa
    24841 gaccttcact gagcgagtat caggcgaaat gtttaaagca cctgcatccc gaatcctctg
    24901 tggacctctg gtcgcagcat tggtcctggc cggagtctca accgcatttg cgagtgggaa
    24961 aatccaaaag aaagaggaag ccgtcgacgc caagcagaca gcgctaacga agccggtttc
    25021 caaggccgat cgcgacaaga aaaagcccga tctggcgatc tctgacgtat tttccggcaa
    25081 gggcgctgat ctgaaggcgg tgacggacgc tcagatcaag gttctccagc gtctgattga
    25141 cgcaacggga gacagcgatc ctgaaaagcc ggatctgctc ttccgcatgg ccgaactcta
    25201 cgccgagcag cagagctact acaacttcaa ggcgcgcgat ctcgacgaga aaatcttcga
    25261 ggcgaaggaa aaaggcgaca ccgcgaagga aaaccaagcc aaggcacagc aggctgacta
    25321 cgaaaagcgt gaaaaagcct ggctgatgga agccgtgaaa aagtacctcg aggtcgccaa
    25381 cggcccgaag taccagaact acacgaagat ggaccaggtc ctcttctacc ttgcgtacct
    25441 cctcacgcag gtgaagaagg aagaccaggc gcgtgtgttc ttcaagcgac tcatcaaaga
    25501 ctatccgcag tcgcagttcc tcccgcacgc ctacctgtcg ttcggtgaat acttcttcga
    25561 gaacaaggat cttgagaacg cactcaagtt ctacgaaaag gtgctcgcct acaacgacag
    25621 cccggtctac ggcttcgcgc tctataagaa gggctgggtc tactacaacc agaccgactt
    25681 caagtccgcg atggcgacgt ttatcgacgt catcgacttt gcgagcaagg gtcgtccggg
    25741 caagccaaag ggggatccgc agctgctgaa agaagcacgc aaggacattg tgcgcaccta
    25801 tgcgcaggtc ggtacgcccg aaaaagcgtg gccgttcttc cagaagtacg gcaaagacta
    25861 cgccccgaac atgctcgaga tccttgccga tctttacaac gcgcaaggca agttcctcga
    25921 cagcatcaag gtctaccgcg agcttatcaa gctgaacccc ggttcggaca agctctgcaa
    25981 ctaccagacg gaaattctca agaacacgct gtcgatgacc ggctcacgcg cggttcctga
    26041 gacggtcaag gaactccagc gtctctcggc gatctacgaa aaggtctccg agatgaagaa
    26101 cgtgaagaag gaatctctcg acgagtgccg cgacaacacc gccggtatgc ttcgtgagct
    26161 tgcgacggtg tggcacaaag aagcgcagaa gactggcgtg atggacacct acgccaacgc
    26221 ttcggacctt tacaaagagt acatccgtcg cttcccgaaa gagaaagaca gctacctgat
    26281 gcacttctgg tacgccgaac ttctcttcaa gctcggctcg atggacaaca aggcttcgag
    26341 cgactggtac tgccaggctg cccccgtcta caccgacgtg gtgaagatgg acacgaaggg
    26401 caagtacctc aaagacgcgg cgtatgctgc ggttatctcg tggaagaact gcctcgatgt
    26461 ctcggatgaa ggcgcggacg ccgccgagga agccaagaag gctctcgctg aaaagcgcgc
    26521 acagcgcaag caggctgagg ccaacaaggg caaggacaaa gacaaggaca agggcgaaga
    26581 tcagcagatg gctgaaaagc ccattcccga gaagatccag aagatgatgg acgccttcga
    26641 tacgtacatc cagtacgtgc cggatgcgcc agaactgccg acgatcaagt accgtcgcgc
    26701 gcgtatcttc ttcgagaaca actacttcga taaggcgatc ccgctgttca aggatctcgc
    26761 cgaaaaccac tcgaagagcg agcttgcgct ttactcggcg aacctgctcc tggagtgcat
    26821 cgccatcaag atcaagctcg acaaagccaa gggcgacgag tgggtcgcga tgatggaaaa
    26881 gcaggtcgac aagttctcgg gcatgccgga gctcaccaag gacaaggagt tcgaggagtc
    26941 gctccagaag atcaagaaca gcctgctgcg catcaagatt gaagtcgtcg aaaagctggg
    27001 cgactacaag caagcgggcg tgctctatct gcagttggcg gagcgcttcc cgaacgatcc
    27061 aaaggtggcg gaactctact tcaacgctgc ggtcaacttc gagcgtgcac ggctcatcgg
    27121 tctggcgatt caggctcgtg agcggctgat ccagctgcgt cccgatgatc cgctggccaa
    27181 gaaggcgatt taccgtctgg gtcagaacta ccagttcatc gcgttctacg agcaagcggc
    27241 tgacaagtac gagacgtttg cgacgaagtt ccccggagag ttcgccgatc cgaaagaaaa
    27301 ggacccggcc aagaaggtcg atgcgccact ggctctcaac accgcggcat tcttccgtcg
    27361 tggtcttggt cagacggaca agtcgatcga gcttgtgaac ctgtttatca agaactacgg
    27421 cggtcgcaag gagttcttcg accaggctgc cggcgtgttc ttcgatatgg gccagattta
    27481 cgagcagcgg aaagactggg ccaaggtcaa gcagcacttc gcggagtacc tgaagtcgtg
    27541 gtcggccaag ggcggtatcg accgtcagat cattgcggaa gtgaagctcg gtgaaatcgc
    27601 gtggcgtgag tcgtgcccac tacagaacca gccgaacggc gcctgccttg aaatcaagcg
    27661 tgtgcgcgcg agtgctgcaa cggctctgca agcgaagaac gctgccgctg cgaagaacaa
    27721 gaaggtcaag aacaagggcc cgctcaagct gggtcagtgc ggtccggaaa cgaagtcgaa
    27781 gatcgcggtt caggatcgtc gtccggcgca agtgaaggag gccatgggcc acttccagaa
    27841 agcgcttgat ctgtacaaag gcggcgcggc tgccaacaac gtgcccaaga acgacgagca
    27901 ggagcgtgac cgtcgcgttg cggtcatgac ctatcacgcc gcgatggcga agatgtatca
    27961 aggtgaccag ctctacgagg agctgatcaa gatgaccgtt ccgacgggcc tcgtcttcga
    28021 tccgcagaag aagaaggaga tggaggcgtc gaccaagaag ttcaagggct ggatcgaagg
    28081 caagaccaag aaactgctcg acgcgcagaa gatttaccag ggcgtcattc tgatgaagaa
    28141 cgccaactgg gccatcgctg cagcggcgcg tatcggtcag ctgttccagg acttcgcaaa
    28201 cggtctcttt accaccgaag tcccaactcc gccgccgacg ccgtcgggta tggagcgcga
    28261 cgagtgggcg gatatgttcc gcaacgccta ctgcgaccag atgaccgacc aggccgaacc
    28321 gctcgatgca aaggcagtcg aaggtctgaa gacctgcctc gacaagtcga cggagctgaa
    28381 ctggtttaac gaatggtcga agctctgcga agaggaactc aaccagatca agccgtctga
    28441 gtacccgctc gcgtcggaaa tccgcgctga gccgggctac gccggtgtga ccttggatgt
    28501 ggcgcagccc ttcatggcgc cagataagta atgaccatgc gcatgaaacg gagacaagct
    28561 gtcatgggta gcggcgctgc ttcacaagtg cgggggattc ggatgggttc tgccgtcctt
    28621 cggttgacga ttgcctcggg gctgcttctg tccgcagacg cggcctttgc gaaaaaggaa
    28681 aaaggtccac tcgacgagat ggtcgagaag tggaaggctg cgcacgagcg gaagaaggac
    28741 agtccgcccg gtccaatcgt ggtgtctgcc gacgattgtc agaagttcgc caaagacatg
    28801 gtcaaggcgg gcgaaaaaga caaaggtcgc gaagcggacg ggatctttaa cgctggcgct
    28861 gtgtacgaag agtgcggcat ggaaaaagat gccgagtcga tgtacaagca ggcgctctcg
    28921 aagaatccga agctggcgcc ggcgatgaac aacctgggcg tcatgatgca gcggcagggg
    28981 cgtgcgcaag aagccatgct gcagttcgag aacgcgatca aggcggaccc caagagcccg
    29041 ctcgcggtat ctgcctataa caaccgtggc gctctgctgt atgaccgtgc gcgccaggcc
    29101 aacaacaaag cgggatttga tgaagcggtc ggtcagcttc gtcgcgcgct tgcactcgat
    29161 gctgagtcga tgccagcgta tcaaatcttg gcgtcaatct attacaccac agctgaagca
    29221 gaccgttcga aactgcgtct tgcccagctt gtgtgtgatg aagccaagaa gatcaatccc
    29281 gattacgctc ctatctacaa tacgcttggt cttatcaagc tacgtagcaa ggacgtgact
    29341 ggcgccctga ccgagttccg gaaggcggcg acgctcgatc cgggtctttc ggaagcgcag
    29401 ctcaacatcg gcgcaatcag cctctcggcc cgtagctaca agcaggcgga agaagccttc
    29461 caagcggtga tcaagaaggt gccgaaccac ttcgacgcaa ccgtcggaat gggcgtggcc
    29521 ctgcgtggtc agcggaaggc cgatgaagcc gagcagtggt acaataaagc gcttcagctt
    29581 caggtcgatc caaaaatgga gccgcgcctg gatgcgaaaa agtgcggtgt tctctacaac
    29641 cttggtctgc tttatcagga ttacaaaaac ggtactcctg aggatatgaa caagggcaag
    29701 gattactata ataa
//
LOCUS       DAORGN010000041        29411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_592_length_29411_cov_6.236817, whole genome shotgun sequence.
ACCESSION   DAORGN010000041 DAORGN010000000
VERSION     DAORGN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 29411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29411
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_29411_cov_6.236817"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKL17_06830"
     CDS             complement(<1..427)
                     /locus_tag="PKL17_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44477.1"
                     /translation="MRGLSVFALLALCASACVTAPPYRGGKFTLSQPLILTADERGYD
                     APQPEVQPLQSGQVMALSLSLDQAAYVYVLQRRGGVLDSVYETPASDGRHGPGQIRIP
                     RGDSYLRVPSLSRDTRLCVLLAAEPIDAQKRACPFERQHL"
     gene            complement(483..1502)
                     /locus_tag="PKL17_06835"
     CDS             complement(483..1502)
                     /locus_tag="PKL17_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44478.1"
                     /translation="MSVAAGSLSVSRPTYRVASLAAALLLSGTLFGGTGCNRTSGTAA
                     AKDDLHLVPKDTDVVFMVNVKQARKSPLWDKFVDKFNQDPKAKADYDEVVKKCQIDPL
                     KQIDSMFVAVPSNVTDSKEFAVLLRGSFAPEKFVTCMQTVAKDKNEPVTEADYNGHKY
                     YTVGKQSSFMTAIPKKAVVLAGSEWVKRSIDLSLGKGENATQNSALGDIIKRTKTSDS
                     LWWAGLVPPQLSEKLGSNPQMGPVRSLRSVSGSVEIAKGLELHAYLDLGSDADATQLK
                     DKATDQLTQLRNLPALKMMGLLSFLDTVKVSSQKASFVVDVNMNQQQVDDLITRLSGM
                     AQQFR"
     gene            complement(1632..2330)
                     /locus_tag="PKL17_06840"
     CDS             complement(1632..2330)
                     /locus_tag="PKL17_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNK44479.1"
                     /translation="MMVWPKHALFALLAAVLALSLSSPAVAKSKKKPAPLPAIELEHL
                     TTHHHLSLQPAPGGGFSKRKMQEVSSFLRCHHTGRRHAMNERLVSILYQVSRHYKNAK
                     LFIVAGYRAPKIAKQKGNPRSPHKRGVATDFQVEGVSIESVRDYLRGNFHNIGVGYYP
                     NSGFVHVDVGRQKDAFWIDYSGPGEKARYSPPSLRRDSRRSPQVAEAGREPAEDSARE
                     KRSSAEPDGDELGG"
     gene            2562..3575
                     /locus_tag="PKL17_06845"
     CDS             2562..3575
                     /locus_tag="PKL17_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44480.1"
                     /translation="MKTLLSTLTSLVLSASLSSAAHAQTLDPSMDPNQQWQDGQGAEM
                     YQAPDPNYQNYQADPNAYQNQVQQFGFFGPHPIAYDQGGGFCNHQGAHEHPYPVFDQN
                     LFRMSSGYAYFIGDVGDFGYSQTGYQYVAEHPIGHIHGGGYCFMSWPHRHWFAPIGVN
                     FVWSNAGYVYNGPWGSDYYSLRPRYVTYYNDYYRRWYLGGRYYSLRPSHTYIGFGWHR
                     PMARPYYGGGGYYRPGYGGAVVRPAPVYRPPVYQNPGYRPGPVYQAPAYRPAPVYQAP
                     AYRAPVYQAPAYRPAPVYSAPMHSAPVYRAPAYSAPVHSAPVYRAPVHSAPAGVHHAH
                     GWR"
     gene            3613..4251
                     /locus_tag="PKL17_06850"
     CDS             3613..4251
                     /locus_tag="PKL17_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK44481.1"
                     /translation="MNPFRVVRSQPEMADDLARIQKLCFPTVGEAEIITAAQYRSHIA
                     TFPEGQLAVIDKQGTVVAGSTSCRCNLDLANYHHTYMEASGDNWLKNHVPDGPWLYGV
                     DIGVHPDYRGQGLSKLLYDARKMLIYSLGLRGILVGGMLKGYGPYRATMSPGEYAAQV
                     ASGTYFDPTLSVQLRRGFHIHGMLPHYIDDPTIDGAAALLVFHNDRLTHADS"
     gene            4273..6291
                     /gene="selB"
                     /locus_tag="PKL17_06855"
     CDS             4273..6291
                     /gene="selB"
                     /locus_tag="PKL17_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNK44482.1"
                     /translation="MSAFRVIGTAGHIDHGKTSLVKALTGIDTDRLKEEKQRGITIEL
                     GFAHLRLPSGATVSVVDVPGHERFIRTMVAGAVGIDAVVLVVAADEGVMPQTREHLDI
                     CELLGVQSGVVALTKCDLVTDDLLALAKQELRQVLAGTFLAHADIVPCSAHSGVGLSD
                     LSQAIERTLHRLPGRDTESAVRLPVDRVFTLKGFGTVVTGTQWSGTLKLGDELVALPS
                     AKTVAKVRGLHVHGKAVSESQAGQRTAVNLSVPLTAIAKGETLVPPGVLVASSVLDVK
                     LRYLPVARLALPRRSRLLVHVGTAQRLCNVTLLDRETLEPGQTAYAQILLSEPLCAMP
                     GDHFVLRGFAPQKHHGTTVGGGLVLRNLAHPQRRKNWHGSDTLTAYRNALQALADESL
                     RAAAIESLVSLHTERRGLHGTSLLDLQRSLPLGLPSIKQALSALLQRQVLRKMDDIPE
                     QEAGAWLSSKSLQTIEEGILSTVKSAIRNSPGSSGLAKEALRSRLFGPLQGITPRLLD
                     AVCQQLVRDGKLFQQREELSTTRDDAKPQDSLPVQAILHAIQKAGLAGPKLEELIVVL
                     PPDVTKQTSKSPPLRPEEIKRLLEPVIRRGDVIRLKDLLFHRSAIDDLRDRLVAFLRQ
                     HREISPTQFKDLVGQSRKYSIPLAEYFDAQRLTLRVGDQRRLRQAPPI"
     gene            6394..7902
                     /locus_tag="PKL17_06860"
     CDS             6394..7902
                     /locus_tag="PKL17_06860"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNK44483.1"
                     /translation="MRRTTSALAGLITLCGLSSAAFATVAQPNGLVVPRDSANGEVQI
                     YTMLQNREPGVNWQTDSNTTPEVFSPLCDFTAELVLRQTGSNLAVGWYNVDPARTVAP
                     ALTEIYQVIPKSSPVGTKITGATIRGDTRYKGGLIGFALMSDGSFTSKFTEKKWNTIC
                     TSCTPQGPWIHSVTYISKATANGFYVAFEDGNPTGSSFNNDGDYNDYVFFFTGLTCQG
                     GGQQCDIPTSKGACRSGVTECLGTGGTTCKPLVQPGQNTEKCDGVDNDCNGIVDDNAP
                     CPANQLCSKGHCADPCGSEVLCPVGQSCELGRCIDTACVGKTCPVTQVCRAGGCVDPC
                     DGIKCPGVTVCSGGVCVDPCASVTCPSGKVCQNGACVQGCDCAPCGSGQACQTSTNKC
                     VSQGCQTMTCGAGDICQNGACVAACTGAVCPSGQACSQGVCQDLPPDTSGGGGSDGGT
                     TEQPPTDGGTIATGCGCRITPSPDRQGLAACVAAFAMLALTARRRITARRRK"
     gene            7985..9130
                     /locus_tag="PKL17_06865"
     CDS             7985..9130
                     /locus_tag="PKL17_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44484.1"
                     /translation="MRGSALWLSVCLLWLSAACTSNRGTNIDDPYAISHGNECSDGQW
                     LCQAGARKQCIRGNWLTRNVCLPNQVCDTSLGCVECSPRLPRQCDGDNVRVCAANGSF
                     GDLIQKCDPGMCKGGTCANTCGENADLVYLVDEQYRLLSFNPRDGKNELKLLGDISCP
                     AGRSFDGGTATPFSMSVDRDARAWVLYNSGEIFWVNTKDLSCKNSGFVPSQSGFETFG
                     MGFVTDGLGTEAETLFVTGGSHLDPGKGRMASIDKKSLMLTPIGSLPTTEYGPELTGT
                     GRGELWAYFPGIGESFVAMMDKGTGMPAQKYPLSPLPGTVRAWAFAHWGGRYYIFVTV
                     FDPMLGRNNSQILMFTPDDGMVQKIMDNLPYIIVGAGVSTCAPVVIG"
     gene            9139..9801
                     /locus_tag="PKL17_06870"
     CDS             9139..9801
                     /locus_tag="PKL17_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNK44485.1"
                     /translation="MDVKDVFLLSQEELGRGHYLRLLRVHLRNQYADGSLSAPYFCEL
                     VDRPAHGTDAVVVGLWRRNSANQDIEVLLRKGLRPALRFGRDPARLAVPDRAPYLLLY
                     EVVAGILENEDRGPEGIKRRAVQEAWEEAGVRLSEADIEPLGAPIFPSPGMSPEAFHL
                     VCAEVKESESEPSGDGSPMEHGAKLLWLSLSEALALCRRGEVEDAKTEVLLHRLERKF
                     RN"
     gene            complement(9819..10607)
                     /locus_tag="PKL17_06875"
     CDS             complement(9819..10607)
                     /locus_tag="PKL17_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44486.1"
                     /translation="MDSKRIALIGVLLCGGSLPFLTGAGGGGCGSFTSTTPAPDVAGN
                     WAISYGNTMSVKVTIGGVSYSQELTPSGGSFTIMHQGKPYAFGVDCSRPEVVCPSEVW
                     PTTVMMDQRDTMYKHRMWVKIPVQQCSGVQVPPQSNQCGTGTPNPECKPVCTGAVSTS
                     TADAFGLISESGDRFDLFLGGGFATNGVNCALLGLSAASAQIVSTGQNIDWQATELRA
                     GTVKTGYAGGCLWAGAVDPMTGKPEALVLGATVEISTPFTGKRL"
     gene            10744..>10923
                     /locus_tag="PKL17_06880"
     CDS             10744..>10923
                     /locus_tag="PKL17_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44487.1"
                     /translation="MRASHRAKASLAALCVVGIGPCGSAWAQQNDAAATPQQVSAVPV
                     LPDGPAAQTAQLPPPP"
     assembly_gap    10924..11023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11024..11890
                     /locus_tag="PKL17_06885"
     CDS             <11024..11890
                     /locus_tag="PKL17_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44488.1"
                     /translation="IRCGGLSIVETVCRYGDFTGIANTYHPNGSVAESLTFVGGILEG
                     LVESYDPVGHLLSRRLFHVGKPLAPGQNVSQIPLGKDPPLPPPPTFDPQVSSLPAGTP
                     EPPSPATSDSTTAPFGGLVGIGGSLQLGVLANQSIAPFAIGGQFLLIPNTGRLRPEFR
                     AGAIYLPRTDYRRIDVPLSIGLQFDLFAAPSSLYVGAALLTQYARRFLPTDIPSPSIE
                     DGWLLGGEGIVGLRVQRSRQSYWLFDVRMGGSGRVDSQPQLLRPQADGPPEPAMGRQF
                     QLLFGISFVGLV"
     gene            11907..12974
                     /locus_tag="PKL17_06890"
     CDS             11907..12974
                     /locus_tag="PKL17_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44489.1"
                     /translation="MDRSRLPFSRRAALLLSFFSLSACGSNQELPDSVKKRNGTVVVE
                     SCALDPWQTATLQSDSARAVLNEVILLCLYVRNGVAEPLAPADQSSVAQVTSSLKQRG
                     YRVRLGVTTGETPGTTPSALEGILVDPAKRAALIASLAGFAPISDGIALAPPTLLPTS
                     EASFRAFVNELAATLPDRRVGLFAPPSSMTPSDVASGDAIKLSALSSSLSRSYLMTLD
                     LHCCDDVPGPTTASDWITRVVTFARSQTNTASLSFSLPLYGTYFGATKTQRQVSYAEA
                     AGMAASRHIEILRREDTGSLYFSFSDAGGVSTVVFDDSHSLLRTLSEVDEGVPADVGV
                     LYYGLGGEDPNLFAALKERMK"
     gene            12971..14272
                     /locus_tag="PKL17_06895"
     CDS             12971..14272
                     /locus_tag="PKL17_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNK44490.1"
                     /translation="MIEVRATAQAAQAAFCDAVIQVAPLGVRDPLANPIFEQALAKNY
                     LIFDAYVSGERRVDLHPIVLTAQQHAEAVRCALTTAKLVSAIASRSHDDPQEAARYGF
                     SPAVRRLAEASHSARDRHFLYRVDLLYGMDGFRACEINADCPGGHNEALGLPLLAQAA
                     GFWSGHNPTSVVAALCDALCALRHGEGTIALIYATAYAEDLQVCALVQRELHRRNIPA
                     VLASPTALRRMGKGLRIGKDRISVLYRYFPTEYMDGQRNLSAIADAVEHGEVRTLSNF
                     AEIYTQSKLAFARVHALSDSLHDSDRAGLRYIPLTHAVTDLDVLDLLSQRSQWVLKRA
                     LGRVGEEVFVGSLLGDEDWKKVIEKARSEHEQGEAWVLQKFVPQRPIESPYGPLLLTL
                     GAYVLDGNFCGYFARVTPESHASHSALCLPVFVLPDTGKEP"
     gene            14272..15237
                     /locus_tag="PKL17_06900"
     CDS             14272..15237
                     /locus_tag="PKL17_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44491.1"
                     /translation="MQTKFAVPLLLLAGSCTTARPPSAEFLARAEQIPPPTSMLLPSA
                     QRAFAAWQLPQDDPWSRFNKWTLLSSLGEQVTAGELPDVRNLVLLQRAELLGARLGAA
                     GLPPDTLWIVDLRGAAAVAFGNAVSHAAPHPVSLVMTFNNWPAADELIPAEETLAALV
                     TMRPRLPEIGTSGSRPVFLLDSWRLAYRDQELAEETIDNRYYLTTSDFPEPAQLVEHG
                     IRRIVYLTETLGGSPDQAVEEDDLHETFLRYSQAGLSLSMLGLDELDDPQPMMLISRR
                     RFYIPRPRTTIVHSPMFYARSHGGFGGPRARPTFHGVLHRSGHGG"
     gene            complement(15526..16350)
                     /locus_tag="PKL17_06905"
     CDS             complement(15526..16350)
                     /locus_tag="PKL17_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44492.1"
                     /translation="MNTTLSTLSPRVAARRSQAQSQSVTSTSAEELVAAPSAENPAQA
                     AATDPLPDVQARLGESQALAELIERARHHDRRARSMLCERLLPFFHRVVAKLSFRFGR
                     VTSETIRQESLDMVQYVACALLEDLDLPGGVLARWRPEQGPLEAWLRPFVTCRALDCL
                     RKRKRPLAELGFGEHLPQALEQDLLSLHRALPGAMVQIEQRDALAKLCERILTEPHLG
                     KEALTLLERLFWLEEDREDVAKSMGLKRNTLDVRVKRIREHLGRLCDELGIPFGQT"
     gene            complement(16673..18904)
                     /locus_tag="PKL17_06910"
     CDS             complement(16673..18904)
                     /locus_tag="PKL17_06910"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK44493.1"
                     /translation="MAAIPITIQVYQGDTLVKTEKLTQETIKIGKISSAHVNLEDDSV
                     SRLHANIEVSPENQVTIVDLSSTKGTFVNGARLGANKPQPLKSGDEVKIGDVRLVVTF
                     EAPAVAAPAPTPAPAAAAAPPPPRPMGPPLPGMRAPGAPVAPPAAAAAAPAHSGGLSI
                     PPAYGPKAFVPQLPPQVREQVELQDGSKAVEVAALFEDAVVEVRHYDDPKGGKVTGAT
                     NGMIYGGIASLAVVFVLFLTNYITVSREVAVEEEWEKENAAAAPDKKKEKPHKSAGSR
                     AHDAAGGILLGAGIAALALGVLRRQKEMQENEFTIGPAPGTTFKTPGENLPVERFPLV
                     RSTGDQYEILLTQKMTGELQVGGKSIQIAELLGSGQARQADVQGAFVMPIPPEGRFSL
                     QLGDSFFNVNSVAKPRAYPVPLDIDWGTQSYTGAVFGAVALFMALIFSVPPDPKSLSL
                     DAFMNDQRFAKLVQKPPEEKPEEIPDWLKKKAEEKDDGGKKAKESEGKMGKKDTNQKN
                     KLYNIKGPQDNKDIKLAKEAAKDAAKNAGVLGLIRSGQMGNMASIFGRDSALGKDASD
                     AMGGLVGTEAGDAYGGGGFGLVGAGGGGGGTGEGTIGLGNIGTVGHGAGGGGYGAKVG
                     ALKGRKAGAPEVVPGTAEVRGSLDKELIRRIIRRHINEVKFCYERELTRTPNMEGRVM
                     VNFTIGPTGGVVASIVQSSTLGNPVVEQCIAGALRRWEFPRPQGGIVVVTYPFVLKAA
                     GGE"
     gene            complement(19262..20743)
                     /locus_tag="PKL17_06915"
     CDS             complement(19262..20743)
                     /locus_tag="PKL17_06915"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNK44494.1"
                     /translation="MATARAWGFAVGLYALAFSPGVATAGGMFVGTHGARPTARGGAF
                     VAGADDLNAIYYNPAGVVLSQPQPEGWSVLFDAGLVMQHVAYTRNDAGVQRPTVVGDG
                     GVIGAAPLVIPQLGFARKFSRPWGSMAFGLGLWIPYTALSRYPEPSYDTEEARRQVPD
                     VAPQRYQLIGLYDGSLSRATLLAVLNPVASFSLLGDKLQLGIGPQLMLLYFRAKLMLN
                     GCPQVMCAPENPDYDALVVAQAFAPVPSLNLGAIYRPIDMLKLGLSFQLPFFVRSATG
                     SVDTRLPVSELFNGAAVQGRDASLAMDLPPILRLGIELTTWQKRLRVELAYTAEFWSI
                     QDSVLVTPKNVSLVNVKGFDTYTLGPVAIDRQMQTTHAVHLGAEATLWKYIGARIGGM
                     FESGAMPDATFTILSPDSHKGMIALGAFVPSVKFAKAEWRFDLGYARIIQPDRIIASE
                     DSKLAPQNPLRPDVGLAPGQGGIGGGRYEVSYDLLTLGFSIIR"
     gene            complement(20743..21696)
                     /gene="trxB"
                     /locus_tag="PKL17_06920"
     CDS             complement(20743..21696)
                     /gene="trxB"
                     /locus_tag="PKL17_06920"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNK44495.1"
                     /translation="MSEGKQIEQVVIIGSGPAGHTAAIYAARANLRPVMLEGFMAGGV
                     AAGGQLTTTTEVENFPGFPSGIMGPELMDRMRAQSEHHGTRIFTETVERVDLSQRPFK
                     VETDSQTVYAESLIIATGATAKRMSIPGEDKLWQRGISACAVCDGALPLFRNQVLVVV
                     GGGDTAVEEATHLAKFGSKVILVHRRGELRASKAMQKRLSADPKVSVLWHHVVEEAIG
                     TTSLQAVRVRNVQSGEAHVIDCRGLFYAIGHVPNTEFLGGQLSLDETGYILTKPGTTQ
                     TSVQGVFAAGDVQDKRWRQAITAAGTGCMAALEAEAFLAGH"
     gene            complement(21811..24135)
                     /locus_tag="PKL17_06925"
     CDS             complement(21811..24135)
                     /locus_tag="PKL17_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK44496.1"
                     /translation="MARPRFWQLWAVCGFLCGCSSVEPGPRYDARCLLPVRFRPDVQL
                     ARKDISLSREQVEHPELIGSWDGFARPGYRRFDEQTSLQGTRYFTLSLPLPPGRYRYG
                     FVVGDELLPDEMNPHSEFAPHPLWIDSAPYEAEWSVFDLPDCRLPSLAVSQLTVDDAS
                     SLTARDGKIVVRFALTDGIEPAQLGKPLVTVKQSGNTLDSPTAQLFTLSDHEVQITIA
                     DLPSGKYQIDLSVSDPIHAAALHKTVQAVVSPQRRAEKESPRGERADTVLYHVFLDRF
                     RGSLGPLAPPATPGMRAGGTLQGVRTAVEQGYFDRLGITTLWLSPLYENAPGTQRGRD
                     GNLYEAYHGYWPAQPRSVEPKLGTAADLEQLVLSAHHHGLRVIFDAVPNHLYVGHPYY
                     QEHGRLSPASQPDPAKKSWFSDGPRACVCGSPGCGWGERIEDCWFDTYLPDVNLRNPD
                     AAAQQSADLLWWLSRFDLDGMRIDAVPMMPRGFTRQVVRSVADQATRRGQDVLIIGED
                     YTGPGDGGRLEIRSFLGDRFDGLHSAFDFPLFWVMVDALAKDRVSLVTLEKQIALSKE
                     VYGGSGAVMGHILNNHDTARFVSEAAGNAGSDPWKSPPAQPTDAEPYLRHMLAMTLLL
                     TLPGIPVLYYGDEVGLAGAGDPDSRRVLPDVLGGGLFSMQQRVLEHVQRLGTLRRCHG
                     ALRKGDRSLLVADNEFSVALHSPPGEGQSGPAIVVIARASGERQLQVRGIPPGRYRDV
                     FSGERLSVDEKSAAGITLRSLKSQVFIREDSPCF"
     gene            24279..25004
                     /locus_tag="PKL17_06930"
     CDS             24279..25004
                     /locus_tag="PKL17_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNK44497.1"
                     /translation="MDKRVKDADAALYDVSDGATIMSGGFGLCGNPENLIEALLRRGT
                     RGLTLISNNCGTDGKGLGKLLQNGQVRKMIASYVGENKTFERMFLEGKLEVELCPQGT
                     LAERMRAAGAGIPAFFTPTGFGTVRAEGKEVRVFPDRLGRQRSYILETALSADYAFVK
                     AWKGDRFGNLVYRKTARNFNPVMATAARITIAEVEQIVEPGEIPPDQVHTPGIFVKRI
                     FQGANYVKPIEQRTVRPRPSSAV"
     gene            25016..25699
                     /locus_tag="PKL17_06935"
     CDS             25016..25699
                     /locus_tag="PKL17_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HNK44498.1"
                     /translation="MAITREQMAQRVASELRDGMYVNLGIGLPTLVANYVPPGMDVIL
                     QSENGMLGTGPFPFEGEEDPDLINAGKQTVTELPGASYFSSAESFGMIRGAHIDICIL
                     GALQVSQTGDLANWTIPGKMVKGMGGAMDLVAGARRVIILMEHVAKVKGAAEGSFDFK
                     VVKECKLPLTAPRCVARIVTDLAVMDVREDGLHLVEVAPGTTVEEVQRYTEPQLILPP
                     GGVVPVMAV"
     gene            25719..26783
                     /locus_tag="PKL17_06940"
     CDS             25719..26783
                     /locus_tag="PKL17_06940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNK44499.1"
                     /translation="MALLSHAGRPGAPPLAVDAVVFGEALVDFFPENPGTKLEDVERF
                     VRHLGGAPCNLAIGLRRQGIPTALVTQVGSDAFGRFIKQKLDSEGVAVDGIASHKSAR
                     TGVTFVSVAATGERSFLFYRHPSADMLVTTAEVTPPLILRGRLFHFGSSSLSREPARA
                     ATLHALELVNKQRKQRIVSCDLNLRPHLWPDIKEAPPLLRRVLSGCDVVKLTDDELPI
                     LCGTESIEAGAQYVRGLGATMVVVTLGDRGAYFDSPQGTAYIAAESVPVVDQTGAGDA
                     FTAGLLATLLVYLNGSGPDDLRERLKALPFDVLKRGCLHGTHLAARVCTALGATTALP
                     HLPPPPPPLVKVSASSKKDF"
     gene            26783..27352
                     /locus_tag="PKL17_06945"
     CDS             26783..27352
                     /locus_tag="PKL17_06945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNK44500.1"
                     /translation="MPPIKIIGLGGSLKPESASVKALRIALSGAVETGAHIELLELHE
                     IALPMFVPDMEPPPQVRRFVDAVSGSAGMIWSSPVYHGSMSGAFKNALDWLELLSKHD
                     PPYLTDKVVGLIATAGGVQGLQAINAMEFAVRSLRGYTHPLTAPIDRAWQLFDKSGNF
                     SDEATIARLRTLGQGVASAARRLGLPHHC"
     gene            27368..28243
                     /locus_tag="PKL17_06950"
     CDS             27368..28243
                     /locus_tag="PKL17_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK44501.1"
                     /translation="MVTVLQEPMRLYDFAWPERFSSRASRHGVLCYFDSGKADDGPPL
                     VMLHALGINFTQWRHVAQILAPQQRLIGIDMPGCGHSAKPRHRWQLSDMTEAVIGLLD
                     ELGIERAMIAGHSFGGRIALELALRHPERVAGLLLLNAAGFIRYPKLLSHVGERLLKP
                     KLVAPIMVALSKPILGTIVAHPTSPSGEFAASVTERFRPEFAWDFAYHACPLLPALLS
                     DVIDELQHLHLPIEVLWGRRDRLLRYRSVEKHLYRIPTARIDVIDDCGHMPNFEHPDL
                     VAKAAERLRSRVRKG"
     gene            28240..29085
                     /locus_tag="PKL17_06955"
     CDS             28240..29085
                     /locus_tag="PKL17_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44502.1"
                     /translation="MTEGRKVRYGDELIYDAEFTRKFIAWIEPIARAYFRAEVRGAEL
                     LPRKQTLLVSHHDGGMMPIDSLFVGKAWHERFSFHRPLHVLVHDMVLRFARPLRRIGA
                     VLADRHNLDRLIESGRSALIYPGAAHETFRPFWERKIITLGNRTGFIKHALHKRILLT
                     PVVSAGVHETFVVLSRGSWLAERLRFRELFRANVFPVVAGLPFGIWLGAFFPQLPLPA
                     KITVQIMPPVDLVPWAERLLGRAWKDSDADEQDVVWTCFYELRDSMQRALDRLYAERR
                     FPILG"
     gene            29181..29390
                     /locus_tag="PKL17_06960"
     CDS             29181..29390
                     /locus_tag="PKL17_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44503.1"
                     /translation="MTNLVVVKATPSADISTDKTMAAGSETAESAKATQRDQVGAPVH
                     LAWREPVRGRALAWFIQAFGGVQFG"
BASE COUNT         5810 a         8954 c         8719 g         5828 t
ORIGIN      
        1 ttaggtgctg ccgttcaaac ggacacgcac gcttttgcgc atcaatcggc tcggcggcaa
       61 gcaggacgca cagccgggta tcccgactga gactgggcac ccgcagatac gaatcaccgc
      121 gcggaatgcg gatctgaccc ggaccgtgac gaccatcgga agccggcgtt tcatagacgc
      181 tgtcgagcac gccaccgcgt cgctgaagca catatacgta ggccgcttga tcgagcgata
      241 gggaaagcgc catcacctgt ccagactgaa gcggctggac ttctggctgc ggcgcatcgt
      301 agccgcgctc atcggcggtg agtatcagtg gctgagacag cgtgaacttg ccgccacgat
      361 agggcggcgc ggtgacgcaa gctgaagcac agagcgcaag gagcgcgaaa acagacaagc
      421 cccgcaagtc gaatccccct gaatcatcaa gatatcaggc cggcgcgcga ctgtggggtg
      481 gtttagcgaa actgctgtgc catccccgac agacgggtga tcagatcgtc gacctgctgc
      541 tggttcatgt tcacatcgac cacgaacgac gctttttgcg acgacacttt gaccgtatca
      601 aggaagctga gaagacccat catcttgagc gccggcaggt tgcgaagctg cgtaagctga
      661 tcggtcgctt tgtccttgag ctgggtcgcg tcggcgtcgg agcccaggtc cagataggcg
      721 tgcagctcca agcctttggc aatctcgacg gaacccgaga cgctgcgcag cgaccgcacc
      781 gggcccatct gcggattgga cccgagcttt tccgaaagct gcggcggcac aagacccgcc
      841 caccacagcg aatccgacgt cttggtccgc ttgatgatgt cgccgagcgc gctgttctgc
      901 gtcgcgtttt cacccttgcc caaagacaag tcgatcgatc gcttgaccca ctcgctgccg
      961 gccagcacca ccgctttttt cggaatcgcc gtcatgaaag acgactgctt gccgacggtg
     1021 tagtacttgt gaccgttata gtctgcctcg gtgaccggct cgtttttgtc cttggcgacg
     1081 gtctgcatgc aagtgacaaa cttttccggg gcaaagctgc cgcgcagaag caccgcgaac
     1141 tctttgctgt cggtcacgtt cgacggcacc gcaacgaaca tcgaatcaat ctgcttgagc
     1201 ggatcgatct gacacttttt gaccacttcg tcgtagtccg ctttcgcttt cggatcttgg
     1261 ttgaacttgt cgacgaactt gtcccacagc gggctcttgc gcgcctgctt gacgttgacc
     1321 atgaacacca cgtcggtgtc tttgggaacc aagtggaggt cgtctttggc cgccgccgtt
     1381 cctgatgtgc ggttgcagcc ggtgccaccg aaaagtgtcc cagaaaggag cagcgccgcc
     1441 gcaagcgagg ccacgcgata ggtcgggcga gaaacggaaa gcgaaccagc agctacggac
     1501 atcgtgggaa tccttgaagt agagagtgtg ccttttgtac gacgcaaaga agcgttctgt
     1561 caacgcgagt ctcgtcgaca accggcaaac gaagggaatg tgtgtggttt tgccaagtcc
     1621 acccaggcag cctacccgcc cagctcatcg ccgtcgggct ccgccgaaga acgcttttca
     1681 cgggcactgt cttctgccgg ttcgcgcccg gcttccgcca cctggggcga acgccgcgaa
     1741 tcgcgacgca gcgacggcgg tgaatagcgc gctttttcac ccggacccga ataatcgatc
     1801 cagaaagcgt ctttttgacg acccacatcg acgtggacaa agcccgaatt gggatagtac
     1861 ccgacgccga tgttgtggaa gttgccgcgc aggtagtcac gcaccgattc aatcgacacg
     1921 ccttcgacct gaaaatccgt cgcgacaccg cgtttgtgcg ggctgcgcgg attgcctttc
     1981 tgtttggcaa tttttggggc gcgatacccc gcaacgataa atagcttggc gtttttgtag
     2041 tgtcgcgaaa cttgataaag gatgctgacc aggcgctcgt tcatggcatg acgacggcct
     2101 gtgtgatggc agcgcagaaa cgacgacact tcctgcattt ttcgcttgga aaaaccacca
     2161 ccgggcgccg gctgtagtga caggtggtga tgcgtcgtca ggtgttccag ttcaatcgcg
     2221 ggcaaagggg ccggcttctt tttggacttg gccacagcgg gcgatgacag agacagcgca
     2281 agcactgccg cgagcagtgc aaacagcgcg tgttttggcc agaccatcat atgctttggg
     2341 gtaacagagg tcggcggcat cggcaagaac tttgtcggcg gcgggccgca agacgtaatc
     2401 ggatgttcac atctgtggcc caaagtagac ggttagaata ttcacgcggc ccacttcatt
     2461 cgcactttct cgacggaaag aggggttgta ccaggaaatg gtcctggtgc cttgctggct
     2521 tggtccgagt ctcgctttga cacctgggca ggaggaacgc catgaagacc ctactttcca
     2581 ccctcacttc gcttgtgctt tcggcttcgc tgagcagcgc cgcgcacgcg cagacgctcg
     2641 acccatcgat ggacccgaat cagcagtggc aggatggtca gggcgccgag atgtatcagg
     2701 cacccgaccc gaactatcag aactaccagg cggacccaaa cgcctatcaa aatcaggtgc
     2761 agcagtttgg ctttttcggc ccgcacccga tcgcgtatga ccaaggtggc ggtttctgca
     2821 atcaccaggg cgcccacgag cacccctacc cggtgtttga tcagaacctg ttccggatgt
     2881 cgagcggcta cgcctacttc atcggcgacg ttggcgactt cgggtacagc cagaccggct
     2941 atcagtacgt cgctgagcac ccgatcggtc acatccacgg cggcggctac tgcttcatga
     3001 gctggccaca ccgtcactgg tttgcgccga tcggtgtgaa cttcgtgtgg agcaacgccg
     3061 gctacgtcta caacggcccg tggggatcgg actactattc gctgcgccca cgctacgtga
     3121 cgtactataa cgactattat cgtcgctggt atctgggcgg tcggtactat tcgctgcgcc
     3181 caagccacac gtacatcggc tttggttggc atcgtccgat ggcgcgcccg tactacggtg
     3241 gtggcggcta ctatcgtccc ggttatggtg gagccgtcgt gcgtcccgcg ccggtttatc
     3301 gtccgccggt ctaccaaaac cccggctacc gtccgggacc ggtctatcag gcccccgcgt
     3361 atcggcccgc tccggtgtac caggctcccg cgtatcgagc cccggtgtac caggctcccg
     3421 cgtatcggcc cgctccggtg tactcggccc cgatgcactc ggctccggtc tatcgcgcac
     3481 cggcttattc ggccccggtt cactcggctc cggtctatcg agccccggtt cactcggccc
     3541 cggcgggcgt tcaccacgca cacggctggc gctaaccgac caatctgtcc cgcttcgtag
     3601 taaaaccccc gcatgaaccc attccgtgtc gtcaggtcgc agcccgaaat ggccgacgac
     3661 ctggcgcgga ttcaaaagct gtgttttccc accgttgggg aagccgaaat cattaccgct
     3721 gcgcagtacc gatcccacat cgcgacgttt cccgaaggtc aactggcggt gatcgataag
     3781 caaggcacgg tcgtcgctgg gtccaccagc tgccgctgca acctcgatct ggccaactac
     3841 caccatacgt acatggaagc ttccggagac aactggctca aaaaccatgt tcccgacggt
     3901 ccatggctgt acggagtcga catcggagtt catccagact accgcggtca gggactcagc
     3961 aagctcctat atgatgctcg caaaatgctt atttattcac tcggtctgcg cggaatcctc
     4021 gtcggaggca tgctaaaggg gtacggtccc taccgcgcca cgatgtcacc tggcgaatac
     4081 gcggcccagg ttgcaagtgg aacctatttt gatccgaccc tgagcgtcca acttcgccgc
     4141 ggttttcaca tccacggaat gcttccgcac tacatagacg atccaaccat tgatggtgcc
     4201 gccgcgctgc tcgtgtttca caacgaccga ctgacgcacg ccgattcctg acttctgcta
     4261 tcgtgcgccg ccatgagcgc tttccgcgtc attggcaccg ccggccacat cgatcacggc
     4321 aagaccagct tggtcaaagc cctgaccggc atcgacaccg atcgactcaa agaagaaaaa
     4381 cagcgcggca tcaccatcga acttggcttt gcccacctgc gtcttccatc gggagcaacg
     4441 gtctcggttg tcgatgtgcc cggtcatgaa cgcttcattc gcacaatggt cgcgggcgca
     4501 gtgggaatcg atgccgtggt actggtcgtc gctgccgatg aaggtgtgat gccacaaacg
     4561 cgcgaacacc tggacatctg cgagctgctc ggggtgcaaa gtggcgtcgt ggcgctgacc
     4621 aagtgtgatc tggtaacgga cgatctgctc gcgcttgcca aacaggaact gcgccaggtg
     4681 ctggccggta cgttcctcgc ccacgccgac attgttccgt gttcggcgca cagcggagtc
     4741 ggtctgtccg atctatccca agccatcgaa cgcacgctgc acagactgcc cggtcgcgat
     4801 acggaatcgg cggtgcgctt gccggtggat cgcgtcttta cgctcaaagg gtttggcacc
     4861 gttgtgaccg gaacccagtg gtcgggaacg ctcaagctgg gcgacgagct ggtggctctg
     4921 ccaagcgcaa aaacagtggc caaagtccgc ggcttgcatg tgcatggcaa agcagtttct
     4981 gaatcccaag cgggacagcg cactgcggtc aatctcagcg ttccgctcac cgcgattgcg
     5041 aaaggagaaa cgctggttcc gcccggtgtg ctcgtcgcat catcggtact ggacgtaaag
     5101 ctccgatatc tgccggttgc acggctggcc ctgccgcggc ggagccgact gctggtgcat
     5161 gtgggaaccg cgcagaggct ctgcaatgtg acgctgcttg atcgcgaaac gctggaaccc
     5221 ggacagaccg cctatgccca gattctgctg tccgagccgc tgtgtgcgat gcctggagat
     5281 cacttcgtgc tgcgtggttt tgccccgcag aaacaccacg gaaccacggt cggcggcggt
     5341 ctggtgctgc gcaacttggc gcatcctcag cgacgaaaaa attggcatgg cagcgacacg
     5401 ttgacggcgt atcggaacgc gctgcaagcg cttgctgatg aatcgctgcg cgctgcggcg
     5461 atagagtctc tggtttcact tcacaccgag cggcgcggac ttcatggaac atcacttctg
     5521 gacttgcaac gatcgcttcc gcttggtttg ccgtcgataa agcaggctct gtcagcactt
     5581 ttgcaacgcc aagtgctgcg gaaaatggac gacattcccg aacaggaagc cggcgcttgg
     5641 ctttcgtcaa aatcactcca gaccatcgag gaaggcattc tctcgacggt aaaaagtgcg
     5701 attcggaatt caccgggatc atcgggactg gccaaagaag cgctgcggtc acggcttttc
     5761 ggaccccttc agggaatcac accgcggctc ctagatgcag tctgtcagca gcttgtccgc
     5821 gacggaaaac tgtttcagca gcgcgaggaa ctttcgacga cacgggacga cgcaaagccg
     5881 caggattccc ttcctgtgca agcgattctg catgcgatcc aaaaggcagg tcttgcggga
     5941 ccaaagctcg aagagctgat cgtcgttttg ccaccggacg tgaccaagca gacatccaaa
     6001 tcgccgcccc ttcgacccga agagatcaag cgactgctcg aacccgtcat ccgccggggc
     6061 gatgtgatcc ggctcaaaga cctgctgttt catcgcagcg ccatcgacga tctgcgtgat
     6121 cgactggtcg cgttcctgcg gcagcatcgc gaaatcagcc caacccagtt caaagacttg
     6181 gtcggacaga gccgcaaata cagcattcct ttggcggaat acttcgacgc gcagcgactc
     6241 accttgcggg ttggcgacca gcgccgtctg cgccaagctc cgcctatata actacgtcga
     6301 taggtagagc cgagttttcg tcaattcagt gaagctgact acatttttat cctgctacga
     6361 tgaaaaggaa tgacttacgt agcaggaggg aacatgagac ggacaacttc ggctctggca
     6421 ggacttatca cgctatgcgg gctgtcgtca gcggccttcg cgacggtggc gcaaccgaac
     6481 ggtctggtgg tgccacgcga ctcggccaac ggagaagtgc agatctacac gatgcttcaa
     6541 aaccgcgaac cgggtgtgaa ctggcagacc gactcgaaca ccacccccga agtcttttcg
     6601 ccgctctgtg actttaccgc cgagctggtg ctgcgccaga ccggatccaa cctcgcggtg
     6661 ggttggtaca acgtcgaccc ggcccgcacc gtcgcccctg cactgaccga aatctaccaa
     6721 gtcattccca agagcagccc ggtgggaacc aagatcacgg gcgcaaccat tcgcggcgat
     6781 acgcgctaca agggcgggct gatcggcttt gccctgatgt ccgacggcag cttcaccagc
     6841 aagttcaccg aaaagaagtg gaacaccatc tgcacgtcgt gcacgccgca gggtccgtgg
     6901 attcactcgg tcacgtacat ctcgaaggca acggcgaacg gtttttatgt cgcgttcgag
     6961 gacggcaatc ccaccggcag cagcttcaac aacgacggtg actacaacga ctacgtgttc
     7021 ttctttaccg gactaacctg ccaaggcggt ggccagcagt gcgacattcc gaccagcaaa
     7081 ggcgcctgcc gcagcggcgt caccgagtgt ctgggcacgg gcggaaccac ttgcaaacca
     7141 ctggtgcagc ccggccagaa caccgaaaag tgcgatggcg tcgacaatga ctgcaacgga
     7201 atcgtcgacg acaacgcccc ctgcccggcc aaccaactct gttccaaagg tcactgcgcc
     7261 gacccctgcg gttcggaagt cctgtgcccg gtcggacaaa gctgcgagct gggccgctgc
     7321 atcgacaccg cgtgcgtcgg caagacctgc ccagtgacgc aagtctgtcg cgccggtggc
     7381 tgcgtcgacc cttgcgatgg tatcaagtgt ccgggcgtca ccgtctgttc gggcggtgtc
     7441 tgcgtcgatc cctgcgcgag cgtgacctgc ccgtcgggaa aagtctgtca gaacggcgcc
     7501 tgcgtccagg gctgtgactg cgctccatgc ggcagcggcc aagcctgcca gaccagcacc
     7561 aacaagtgcg tatcgcaagg ctgccagacc atgacctgcg gagccggcga catctgtcaa
     7621 aacggcgctt gcgtcgcggc ctgcaccggc gcggtctgcc caagcggcca agcgtgcagc
     7681 caaggcgtct gccaagacct gcctccggac accagcggcg gcggcggcag cgacggcggc
     7741 acaaccgagc agcccccaac cgacggtgga accatcgcca ccggctgcgg ctgccgcatt
     7801 acgccaagcc ccgaccgcca aggactcgcg gcctgcgtgg ctgcgtttgc tatgctcgcg
     7861 ctcaccgccc gtcgccgcat cacggctcgc cgccgcaaat agcaccgcgc atacgtaacc
     7921 tttgacgacc gccgcagggt cggaatatcg tccggcctca tcttccatct tgctggaggt
     7981 cgcgatgcgc ggttccgccc tgtggctgtc ggtctgtttg ctctggctct ctgctgcatg
     8041 cacatcaaac cgcggaacca acattgatga cccctatgcc atttcgcatg gcaatgaatg
     8101 cagcgacggt cagtggctgt gccaagccgg tgcgcgcaaa cagtgcatcc gcggcaactg
     8161 gctgacccgc aacgtctgtc taccaaacca agtctgcgat acgtcgcttg gctgcgtcga
     8221 gtgttcgcct cgcctgccgc gccagtgtga cggcgacaac gtccgggtct gcgccgccaa
     8281 cggcagcttt ggcgatttga tccaaaagtg cgaccccggc atgtgcaaag gcggaacctg
     8341 cgccaacacc tgcggtgaaa atgccgactt ggtctatcta gtcgacgagc aataccggct
     8401 cctgtcgttc aatccgcgcg atgggaaaaa cgaactgaag ctacttggcg acatcagctg
     8461 cccggcggga cgatcgtttg atggtggcac cgcgacgccg ttttcgatgt ccgtcgatcg
     8521 agatgcccgc gcctgggtgc tttacaacag cggcgaaatt ttttgggtca acaccaagga
     8581 tctgagctgc aaaaacagtg gctttgtccc cagccaaagc gggtttgaaa cattcggaat
     8641 gggctttgtc accgacggac ttggcaccga agcagagacc ctgtttgtga ccggcggttc
     8701 ccatcttgat ccaggcaaag gacggatggc cagcatcgac aaaaagtcgc tcatgctgac
     8761 tccgattgga tcgctgccga cgacagaata cggaccggag ctgaccggga ccggacgcgg
     8821 tgagctgtgg gcgtacttcc ccggaatcgg tgagtcattt gtcgcgatga tggacaaagg
     8881 aaccggaatg ccggcgcaaa agtatccgct gtcgccgctg ccaggcaccg tgcgcgcttg
     8941 ggcatttgcc cactggggag gccgctatta catctttgtc acggtgttcg atccgatgct
     9001 cggacgcaac aactcccaga ttttgatgtt tacgcccgat gacggaatgg tccaaaaaat
     9061 catggacaat cttccgtaca tcatcgtcgg tgcgggcgta tccacctgcg cccctgtggt
     9121 gattgggtaa ggcgacccgt ggacgtcaaa gacgtatttt tgctttcaca agaggagctt
     9181 gggcgcgggc actacctgcg gcttctgcgc gttcacctgc gcaaccagta tgccgatggg
     9241 agtctgtctg cgccctattt ttgtgaactg gtcgatcgtc cggcgcatgg caccgatgcg
     9301 gtggtggtcg gtctttggcg acggaacagc gccaatcaag acatcgaggt gctgctgcgc
     9361 aagggactcc gtccagcgct ccgttttggt cgagatccgg cccggctggc ggttcccgat
     9421 cgggccccct atctgctttt gtatgaagtc gttgccggca ttttggaaaa cgaagatcgg
     9481 ggtccggaag gaatcaagcg gcgcgcggtg caagaagcct gggaagaagc cggtgtccgt
     9541 ctgagcgaag ccgacatcga gccgctggga gcaccgattt tcccaagccc cggcatgtcc
     9601 cccgaagcgt tccatttggt ctgtgccgaa gtcaaagaaa gcgaaagtga gccaagcgga
     9661 gacggttccc ccatggaaca cggcgccaag ctattgtggc tgtcgctttc agaagcgctt
     9721 gcgctgtgtc ggcgtgggga agtggaagac gcgaagactg aagtgctgct tcatcgactg
     9781 gaacgcaagt tcagaaactg aatctgcgtt cattttttct acagccgctt gccggtaaac
     9841 ggagtggaaa tttcgacggt cgccccgagc acgagcgctt ccggtttgcc ggtcatcggg
     9901 tcgaccgcac ctgcccacag acagccgccg gcatagcctg tcttgacggt tccagcacgc
     9961 agctcggtgg cttgccagtc gatgttctgt ccggtcgaaa cgatctgtgc gctcgccgcg
    10021 gaaagaccaa gcagcgcgca gtttacgccg tttgtggcaa agccgccgcc caggaacaga
    10081 tcgaagcgat caccggattc agaaatcaga ccaaatgcat cagccgtgct ggtggagact
    10141 gcccccgtac agacaggctt gcactcggga ttgggcgttc cggttccgca ctgattgctc
    10201 tggggcggca cctggacgcc gctgcactgc tgcaccggaa ttttcaccca catccgatgt
    10261 ttgtacatcg tatcgcgctg atccatcatc actgtggtcg gccacacttc gctcgggcag
    10321 accacttcgg gacgggaaca gtcgacgcca aacgcatacg gcttgccctg atgcatgatc
    10381 gtaaaggaac caccgcttgg cgtaagttcc tgcgaatagg acacgccgcc aatggtgact
    10441 ttgacgctca ttgtgttgcc gtaggaaatc gcccagttcc ctgcaacatc aggagccggt
    10501 gtcgtcgacg taaacgagcc acagccgcca ccacctgccc ccgtcaggaa gggaagcgaa
    10561 ccaccgcaga gcagaacacc gataagcgca attcgttttg agtccatagg agttcctcca
    10621 tggttaggga gtccggaaac ggcgtgagag tatgcctaac gtggcctgtc ggcaacctcc
    10681 ttgtatgcgc tgcgtttttc gcaaatttct ttcgagccag cgactgctga tacactgaac
    10741 cgtatgcgcg ccagccaccg tgcgaaagcc agccttgccg ctctgtgtgt cgtcggaatc
    10801 ggaccgtgcg gcagcgcatg ggcacagcaa aacgacgccg cagctacgcc acaacaagtc
    10861 agtgcggtcc cagtgctccc tgacggacct gcggcccaga ccgcccagct tccgccacca
    10921 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatccgct gtggtggact
    11041 gtcgattgtc gaaacagtct gtcgctacgg cgactttact ggcatcgcaa acacgtacca
    11101 tccaaacggc agcgtggccg agtctctgac gtttgtgggc ggcatcctcg aagggcttgt
    11161 cgaatcctac gatccggttg gacaccttct gtcgcgtcgt ctgtttcatg tgggaaaacc
    11221 gctcgcgccc ggacagaacg taagtcagat accgcttggc aaagatccgc cactgccacc
    11281 gccgccgacc tttgatccac aggtttcgtc actgccggca ggaacaccgg agccgccgtc
    11341 gcccgccacg tccgattcca cgacggcccc atttggcgga ctggtgggga ttggcggatc
    11401 acttcagctc ggtgtgttgg ccaatcagtc gatcgctccg tttgcgattg gcggccagtt
    11461 tctgctgatt cccaacaccg gacgactgcg ccccgagttc cgcgccggcg cgatctatct
    11521 tccccgaacg gattaccgac gaatcgatgt gcctttgtcg atcgggttgc agttcgatct
    11581 gttcgcagca ccaagctcac tgtatgtggg cgcggcactc ctgacccagt atgcgcgtcg
    11641 attcctgccc acggacatcc cttctcccag catagaagat ggctggctgc tcgggggaga
    11701 aggaatcgtt ggactccgcg ttcagcgctc taggcaaagc tactggctgt tcgatgtccg
    11761 gatgggagga agcggacgtg tcgattccca gccacagctt ctgcgccccc aagcagacgg
    11821 tccgccagag ccagcgatgg gacgtcagtt tcagcttctg tttggcatct ctttcgtcgg
    11881 cctggtttga gcctaggagt cttacgatgg atcgctcacg tcttcctttt tcgcgtcgcg
    11941 ccgcgctgct gctgtctttc ttttcgctgt cggcctgcgg tagcaatcag gaacttcccg
    12001 acagcgtgaa gaagcggaat ggaacggtgg tggtggaaag ctgcgcgctt gatccttggc
    12061 aaactgcgac gctgcaatcc gatagcgcac gcgcggtgct aaacgaagtg atcctgctgt
    12121 gtctgtacgt tcgcaatggc gttgccgaac cgcttgcgcc tgctgatcag tcgagcgtag
    12181 cgcaagtcac aagctcgcta aaacagcgcg gttatcgcgt ccgtttggga gtcaccacag
    12241 gagagactcc cggcacgacc cccagtgcac ttgaagggat cttggtcgat ccagcaaaac
    12301 gcgcggcgct cattgcatcg ctggccggat ttgccccgat ttccgacgga attgcactgg
    12361 cgccccccac gcttttgccg accagtgaag ccagcttccg ggcgtttgtg aatgaactcg
    12421 ctgcaaccct tccggatcgc agagtcggcc tgtttgcccc gccatcgagc atgaccccaa
    12481 gcgatgttgc atcgggggat gcgatcaagc tgtccgcgct cagcagctcg ctttcccgca
    12541 gctaccttat gacgctcgat ctgcactgct gcgatgacgt tcccggacca accacggcgt
    12601 cagactggat cacccgcgtg gtgacgtttg cccgatccca gacgaacaca gcgtcgcttt
    12661 ccttttcgtt gccgctgtac ggaacgtact ttggtgcgac caaaactcag cgtcaggtgt
    12721 cgtatgcaga agcggcagga atggcagcca gtcgacacat cgaaattctg cgccgcgaag
    12781 atactggatc gttgtacttc agtttttccg acgcgggcgg tgtttccacg gtggtctttg
    12841 atgattccca cagcctgctg cgcacactat cggaagtcga tgaaggggtt cctgcggatg
    12901 tcggcgtgct gtactacgga cttggcggcg aagatccaaa cctgtttgca gcgctaaagg
    12961 agcgcatgaa gtgatcgaag tacgcgccac cgcgcaggct gcacaagcgg cattctgcga
    13021 tgcagtgatc caggtcgctc cgctcggtgt gcgcgatccg ctggccaatc cgatttttga
    13081 acaagcgctg gcgaaaaact atctgatctt cgatgcctac gtcagcgggg aacgccgggt
    13141 cgatctccat ccaattgtgc tcacggcgca gcaacatgcc gaagccgtcc gctgcgcgct
    13201 gaccacggca aagctggttt cagccatcgc atcgcgttcg catgatgatc cgcaggaagc
    13261 agcacgctac ggcttttcac cagcggttcg ccgtctggct gaagcgtcgc acagtgcgcg
    13321 cgatcgccac tttttgtatc gtgtggatct gctgtacgga atggatggtt ttcgcgcctg
    13381 tgagataaac gccgactgtc ctggtggaca taatgaagca cttggtctgc ctcttttggc
    13441 gcaggctgcg ggcttttggt ctggacacaa tcccacgtcg gtggttgcgg cgctgtgtga
    13501 tgcgctctgc gcgctgcgcc atggcgaagg aaccatcgcg ctgatttacg cgacggcgta
    13561 tgcggaagac ttgcaggtct gcgcactggt ccagcgagag ctccacagac gcaacatccc
    13621 tgcggtgttg gcatcaccga ccgcactgcg ccggatggga aaaggtctgc ggattggcaa
    13681 agatcgcatt tcggtgctct atcgctattt tcccactgag tacatggacg gtcagcgaaa
    13741 cctctctgca attgctgatg ccgtcgagca tggcgaagtc cgaacgcttt caaactttgc
    13801 tgaaatctac acccagtcca agctggcatt tgcgcgcgtg catgcactgt ccgattcctt
    13861 gcatgacagc gatcgtgctg ggcttcggta cattccgctg acccatgcag tgaccgatct
    13921 ggatgtgctg gatctgcttt cccaacgatc gcagtgggta ctaaaaagag cgctcggtcg
    13981 cgtcggagaa gaagtctttg ttggctctct gcttggggat gaagactgga aaaaagtgat
    14041 cgaaaaagca cgcagcgaac acgagcaggg tgaagcctgg gttttgcaaa agtttgttcc
    14101 gcaaagaccg atagagagtc cctacggacc actccttttg actttgggag cctacgttct
    14161 cgacggaaat ttttgtggtt actttgcgcg cgtgaccccc gaaagtcatg catcccatag
    14221 cgcactctgt ctgccggtgt ttgtactgcc cgataccggg aaggagcctt gatgcagacc
    14281 aagtttgctg ttccactact tctgctcgcg ggttcctgca ccacggcccg gcctccgtcc
    14341 gcggagtttc tggcgcgggc cgaacagatt ccgccaccga cgagcatgct gctcccatcg
    14401 gcacagcgtg cgtttgcggc gtggcagctt ccgcaagatg atccatggtc gcgctttaac
    14461 aaatggacgc ttttgtcatc gctgggtgaa caggtgacgg cgggtgagct tccggatgtg
    14521 cgcaacttgg tgctgctcca gcgcgcggag cttctcgggg cgcgtcttgg cgcagcgggt
    14581 ctgccacccg acaccctgtg gattgtcgat ctgcgcggag cggctgccgt cgcatttggc
    14641 aatgcggtca gtcacgcggc gccccatccg gtgtcactgg tgatgacgtt taacaactgg
    14701 cccgcagcgg atgaattgat tcccgcggaa gagacgctgg cggcgctggt gacgatgcga
    14761 ccgcgactgc ccgaaatcgg gacaagtggg agtcgtccgg tctttttgct cgattcgtgg
    14821 cggctcgcgt atcgcgacca ggaacttgcc gaggaaacca tcgacaatcg ctactatcta
    14881 acgacgtcgg attttcccga accggcccag cttgtcgaac acggcatccg acggattgtc
    14941 tacctgaccg aaacgctcgg cggaagtcct gatcaagcgg tggaggaaga tgacctgcac
    15001 gagacgtttt tgcgctactc gcaagcgggt ctgtcgctgt cgatgctggg attggacgaa
    15061 ctggatgatc cacagccgat gatgctgatt tcacgacgtc gtttctacat ccccaggccg
    15121 cgtaccacca tcgtccacag tccgatgttc tacgcccgtt cccacggtgg tttcgggggt
    15181 ccgcgcgctc gtccgacgtt ccacggggtg ctccaccgat ccggtcacgg cggatagttc
    15241 gtgctgcatc gtcgcctgcg atacgatgtc gccgatctct ttttccgaca gcggacgaaa
    15301 cagatcaaca agccctggcg aaagcgcggg gtcgcgggcc aacgcggcag tttgttcgtc
    15361 ggatagctcg ccacgctgaa gatcggcaag ctgcgcttct ttgtccgcaa aagccgcgcg
    15421 ttccacgata tgggaaagtt tgatggggtc catgcaacct ggatgcgtta cggaacgcac
    15481 gcgcaggttg tgacgcaagt cccatcgatt gcaggtgtgc gcagcttagg tttggccaaa
    15541 cggaatgcca agctcgtcgc agagccgacc caggtgctcg cgaatccgct tcacccgcac
    15601 atcgagtgta ttgcgcttga gccccatgct tttggcgaca tcctcgcggt cttcttccag
    15661 ccaaaacagc cgctccagca gcgtgagcgc ttctttgccc aggtgcggtt cagtcaaaat
    15721 ccgctcacac agcttggcca gggcgtcgcg ctgctcgatc tgaaccatcg cgcccggcag
    15781 agcacggtgc agcgacagca gatcctgttc aagcgcctgc ggcaaatgct caccaaatcc
    15841 aagctctgcc agaggccgct tgcgcttgcg cagacaatcg agcgctcgac aggtcacaaa
    15901 cggacgcagc cacgcctcca gcggcccttg ctctggacgc cagcgggcca gcacgccccc
    15961 cggcaggtcc aggtcttcca acagcgcgca ggccacgtac tgcaccatgt cgagcgactc
    16021 ttgccgaatc gtttcgctgg tcacgcgccc aaaccgaaag ctgagcttgg ccaccacgcg
    16081 atgaaagaac ggcagcagcc gctcgcacag catgctgcgg gcacgccggt cgtgatggcg
    16141 ggcgcgctcg atcagctcgg ccagggcttg ggattcaccc agtcgggcct gcacatcggg
    16201 cagcggatcg gttgccgccg cttgggccgg gttttcagcg gaaggcgccg cgacaagttc
    16261 ttcggctgac gtgctggtga cggactggct ttgcgcctgg cttcggcgag cggccacgcg
    16321 cggagacaaa gtggaaagag tggtgttcat gaccgttctc tttcgcaagc gccgggccag
    16381 cccattttct gacgaacatc ttgcgttctc gcgcaaactc cgtcgccatt gcccatgggc
    16441 agccggcaga cacgccctgg gcacgccggc tggttgcaaa gcgagtttca agctcacaag
    16501 tcgcgtatga aaacgccaac gccactgtcc acactacgaa gcaggtccgc catggactga
    16561 cagtgatggg aatcgagaga aaggagcggg agggaaagga gcgggaggga aaggagcagg
    16621 aggggagcgg ctgggtaccg aggggctggc acccagcagg ccgacgtgaa gcttattcgc
    16681 cgccagcagc cttgaggacg aacgggtagg tcacgacgac gataccaccc tggggccgcg
    16741 ggaactccca gcgacggagc gcaccagcga tgcactgctc gacgacgggg ttaccgagtg
    16801 tcgacgactg cacgatggaa gcaacaactc cgcctgtcgg tccaatcgtg aagttcacca
    16861 tgacgcggcc ttccatgttc ggggtgcggg tcagctcgcg ctcgtagcag aacttcactt
    16921 cgttgatgtg gcgacgaatg atgcggcgaa tcagttcctt atcgagcgag ccacgaactt
    16981 ccgcggtgcc ggggacgact tcaggagcgc ccgccttgcg gcctttgagg gcacccactt
    17041 tggcgccgta gccaccacca cccgcgccgt gaccgacggt gccgatgttg ccaagaccga
    17101 tcgtgccttc acccgtacca ccaccgccgc cgccggcgcc gacgaggccg aagccaccgc
    17161 caccgtatgc gtcaccagct tccgtaccga cgagaccacc catggcgtcc gacgcatcct
    17221 taccgagtgc cgagtcacga ccgaagatcg aggccatgtt gcccatctga cccgagcgga
    17281 taagaccgag aacaccggcg ttcttcgccg cgtctttggc cgcttctttg gccagcttga
    17341 tgtccttgtt atcttgcggg cccttgatgt tgtagagctt gttcttctgg ttcgtgtcct
    17401 ttttgcccat cttgccttcg gactctttgg cctttttgcc gccgtcgtcc ttctcttcgg
    17461 ccttcttctt cagccagtcg ggaatttctt cgggcttttc ctcgggtggc ttctgcacaa
    17521 gcttcgcaaa gcgctgatcg ttcatgaacg catcgagcga cagcgatttc gggtcaggcg
    17581 gcaccgagaa gataagcgcc ataaacagcg caaccgcacc aaacaccgcg ccggtatacg
    17641 actgcgttcc ccagtcgatg tcgagcggca ccggatacgc gcgcggcttg gccaccgagt
    17701 ttacgttgaa gaagctgtcg ccaagctgaa ggctaaagcg accttccggc ggaatcggca
    17761 tcacaaacgc gccctgcacg tcggcctgac gcgcctgacc cgagccgagc agctcggcga
    17821 tctggatgct cttgccaccg acctgaagct caccggtcat tttttgggtg agcaggatct
    17881 catactggtc gccagtcgaa cgaaccagcg ggaagcgctc gacgggcagg ttttcgccag
    17941 gggtcttgaa ggtcgtgcca ggagccggtc caatcgtgaa ctcgttttcc tgcatttcct
    18001 tctgccgacg cagcacgccg agcgcgagcg ccgcgatgcc agcaccgagc aggattccgc
    18061 ccgcagcgtc gtgcgcccgg ctgcctgctg acttgtgcgg cttctctttt ttcttgtcgg
    18121 gagccgccgc ggcattttct ttttcccact cttcctcgac ggcaacttca cgcgacaccg
    18181 tgatgtagtt ggtcaggaac aggacgaaga ccaccgccaa agaggcgatg ccgccataga
    18241 tcatgccgtt tgtggcgccc gtgaccttgc cacctttcgg atcgtcgtag tggcgaactt
    18301 cgacgacggc atcttcgaac agcgcggcca cttcgaccgc cttcgagccg tcctgaagtt
    18361 ccacttgctc acgaacctgc ggcgggagct ggggaacaaa cgctttgggg ccataggccg
    18421 gcggaatcga cagaccaccg gagtgggccg gcgcagcagc cgcagccgca ggcggggcaa
    18481 ccggagcgcc cggcgcacgc ataccaggca gcggcggacc catcggacgc ggtggcggag
    18541 gagccgcagc agccgcagga gctggcgtcg gtgccggagc agcgaccgca ggtgcttcaa
    18601 acgtcacgac caggcggaca tcgccgatct tgacttcgtc accgctcttg agcggctgtg
    18661 gcttgttggc cccgagccgc gccccgttca caaacgtgcc cttcgtcgag ctgaggtcga
    18721 cgatggtcac ttggttttcg ggagagactt cgatgttggc gtggagccgg ctcaccgagt
    18781 cgtcttcgag gttgacgtgg gccgacgaga tttttccgat cttgatcgtc tcttgggtca
    18841 gcttttcggt cttcaccaag gtatcgccct ggtagacctg aatcgtaatg gggatggcag
    18901 ccatctatgt cctccctgcc tggttctttc ctgttagcca gtcacagttt cggacacctt
    18961 ttggttttct gggttccaaa aaattttctt gttgttcaaa aaacccgtgc taggaaggtc
    19021 gaaccaatgc gagcggaaca gctccgtcgg gaatcgcagt ggagcaacac aaccacacgt
    19081 cgcacaagct gcgccatcgc ccgaccccac cgctgatctg ccgacaagca aaaaaacgac
    19141 cgcgacccca aacaggttgg cgatcgaaat cggcctgatc gcttgcggat cgtgttgcgc
    19201 acagccgatt ggacacaggc ttcgcagcta ggttcccaac gcctgctttg ccgatcgctg
    19261 cttatcgaat gatggaaaaa ccgagcgtga gaaggtcgta gctgacttcg tagcgaccgc
    19321 cgccgattcc gccttgacct ggggccaggc ccacgtcggg acgcagcgga ttttgtggcg
    19381 cgagtttgct gtcttcggac gcgatgattc gatcaggctg gatgatgcgg gcgtagccga
    19441 ggtcgaaacg ccattcggct tttgcgaact ttacagacgg gacaaatgca ccaagtgcga
    19501 tcatcccttt gtgactatcg ggcgataaga tcgtgaacgt ggcgtcgggc attgcgccgc
    19561 tttcgaacat accgccgatg cgcgccccga tgtatttcca cagcgtcgct tcggcgccca
    19621 gatggaccgc gtgcgtcgtc tgcatctgcc tgtcgatcgc gacgggcccc agcgtatagg
    19681 tgtcaaaacc ctttacgttt acgaggctga cgtttttcgg cgtgaccaag acgctgtcct
    19741 ggatgctcca aaactcggcg gtataggcca gctcgacgcg cagccgcttt tgccacgtcg
    19801 tcagctcgat gccaagccgc aagatcggcg gcaagtccat cgccagactc gcatcgcgac
    19861 cctgcaccgc tgcgccgtta aacagctcgc tcaccggcag gcgggtatcg acgctgcccg
    19921 tcgcgctgcg cacaaaaaat ggcagctgaa acgacagccc cagcttcagc atgtcgatgg
    19981 gccggtagat cgcgcccagg ttcaaagacg gcaccggcgc gaatgcctgc gcaacgacga
    20041 gcgcatcgta gtcggggttt tccggcgcgc acatcacctg cgggcagccg tttagcatca
    20101 gcttggcgcg aaagtagagc agcatcagct gcggaccgat gcccagctgc agcttgtcgc
    20161 cgagcagcga aaagctggcg acggggttta gcaccgcaag cagcgtcgcc cgcgacagcg
    20221 atccgtcgta cagcccgata agctgatagc gctgcggtgc cacgtcggga acctggcggc
    20281 gcgcttcctc ggtgtcatac gacggctcgg gatagcgact gagcgcggta tagggaatcc
    20341 acaaccccaa accaaacgcc atcgatcccc acggacgcga aaacttccgc gcaaagccaa
    20401 gctgcggaat gaccagcggc gccgcgccga tgactccgcc gtcaccgacg accgtgggtc
    20461 gctggacgcc cgcgtcgttg cgggtgtagg cgacgtgctg catgacgagc ccagcgtcga
    20521 aaagtacact ccagccttcg ggttgcggct ggctgagcac gacaccggcg gggttatagt
    20581 aaattgcgtt cagatcatca gcgccagcga cgaaagcccc gcctcgcgca gtgggacgag
    20641 cgccgtgggt tccgacgaac atgccacccg ccgtcgctac accgggggaa aacgccagcg
    20701 catacaggcc gacagcgaat ccccaggccc gcgccgtcgc catcagtgtc ccgccaaaaa
    20761 ggcttccgcc tcaagcgctg ccatgcagcc cgtgcccgca gcggtgatcg cctgacgcca
    20821 gcgcttgtcc tgcacgtcgc ccgcggcaaa cacaccctgc accgacgtct gggtcgtgcc
    20881 cggcttggtc aggatgtaac cggtttcatc cagcgagagc tgaccgccga gaaactccgt
    20941 attgggaacg tgacctatcg cataaaacag gccgcggcag tcgatgacat gggcttcgcc
    21001 gctttgcaca ttgcgcaccc ggaccgcttg cagcgacgta gtgccgatcg cttcttcgac
    21061 gacgtgatgc cacagcaccg acactttggg gtccgcactg agccgctttt gcatcgcttt
    21121 cgacgcccgc agctcgccgc gacgatgaac caggatgact ttgctgccaa acttggcaag
    21181 atgagtcgct tcctcgaccg cggtatcacc accaccgacg acaacgagca cttgattgcg
    21241 aaacaaaggc agcgcgccat cgcagaccgc acaggcgctg atgccacgct gccacagctt
    21301 gtcttcgccg gggatcgaca tccgcttggc agtggccccc gtcgcgatga tcagtgactc
    21361 ggcgtacacc gtctggctgt cggtctcgac tttgaagggg cgctggctca gatcaacgcg
    21421 ctcgacggtt tcggtgaaaa tgcgggtgcc gtggtgttcg gactgggccc gcatgcggtc
    21481 catcagttcg gggcccatga tgcccgacgg aaagccgggg aagttttcga cttcggtcgt
    21541 cgtcgtcagt tgaccgcccg cggcgacacc accggccata aagccttcca gcatgaccgg
    21601 gcgcaggttc gcacgagccg cgtaaatggc cgcagtgtgt ccggcgggac cggagccgat
    21661 gatgacgact tgttcgattt gtttgccttc gctcatgggt atttttgctc gatttgtggt
    21721 ggtaggccgc cgagcgtagc caaaaccacc gtgtttgtta agcgcgtcgc cgcccagcaa
    21781 gcagcccggc gactgacgac gacaagaaga tcaaaaacac ggcgagtctt cgcgaatgaa
    21841 gacctggctt ttcagcgacc gcagggtgat tccggctgcg gatttttcgt cgacagacaa
    21901 gcgctcgcca ctgaacacat cgcgatagcg accgggcgga atgccgcgca cttgcagctg
    21961 ccgttccccg ctggcgcgcg caatcacgac gatggcaggg ccgctctgtc cttcgccggg
    22021 cggactgtgc agcgcgaccg aaaattcgtt gtcagcgacg agtagtgagc gatccccttt
    22081 gcgcagcgcc ccgtgacagc gacgcagagt cccaagccgc tgcacatgtt ccagcacccg
    22141 ctgctgcatc gaaaaaagcc cgccgccgag cacatccggc agcacccgtc gcgaatctgg
    22201 atctccggcc ccggccaagc cgacttcatc gccgtaatac agcaccggaa tccccggcaa
    22261 ggtcagaagc agcgtcatcg ccagcatgtg gcgcagataa ggctccgcgt ccgtcggctg
    22321 ggctggtggc gacttccacg gatcactgcc ggcattgccc gccgcttccg acacaaaacg
    22381 cgccgtatcg tggttgttca ggatgtgccc catcaccgcg cccgatccgc cgtagacctc
    22441 tttcgacaga gcgatctgtt tttccagcgt cacaagcgac acccgatctt tggccagcgc
    22501 gtcgaccatg acccaaaaca gcggaaaatc gaatgcactg tgcaggccgt cgaatcgatc
    22561 gcctaaaaag ctgcggattt ccagccgccc gccgtcgcca ggtccagtgt aatcctcgcc
    22621 gatgatgagc acgtcctgac cgcgacgggt cgcttgatcc gcgacgctgc gcaccacctg
    22681 gcgagtaaaa ccgcgcggca tcatcggcac cgcatcaatc cgcattccgt cgagatcaaa
    22741 gcgcgaaagc caccacaaca ggtcggcaga ctgctgggct gccgcgtcgg gattgcgcag
    22801 gtttacatcg ggtagatagg tatcaaacca gcagtcctcg atccgttcgc cccagccaca
    22861 acccggactg ccacagacgc aggcacgcgg accgtcggaa aaccaggatt tcttggccgg
    22921 atcaggctga cttgcggggg acagtctgcc gtgctcttgg tagtacggat ggccgacgta
    22981 caagtggttt ggcactgcat caaaaatcac gcgcagaccg tggtgatgcg ccgacaggac
    23041 aagctgttcc agatccgccg ccgttccgag cttgggctcg acggatcgcg gctgggccgg
    23101 ccagtatccg tgatacgcct cgtacagatt cccatcgcgg ccacgctggg ttccgggcgc
    23161 attttcataa agcggagaca gccacagcgt ggtgattccc aagcgatcaa aatagccttg
    23221 ctcgacggcg gttcggacgc cttgcagggt tcctccggcg cgcattcccg gtgtcgcagg
    23281 cggcgccagg ggccccaagc tgccgcgaaa acgatccaaa aacacatggt aaagcaccgt
    23341 atccgcacgc tcaccgcggg ggctttcctt ttcggcgcga cgctgcggtg acaccaccgc
    23401 ttgcaccgtc ttgtgcagcg cagcagcgtg gatcggatcg ctcaccgaca ggtcgatttg
    23461 gtacttcccg ctcggcaagt cagcgattgt gatctggact tcatgatccg acagcgtgaa
    23521 aagctgcgcc gtcggagaat cgagcgtgtt tccactctgc ttgacggtga ccagcggttt
    23581 cccaagctgg gccggttcga ttccgtctgt cagcgcaaac cgcacgacaa ttttcccgtc
    23641 gcgcgccgtc aaactgctcg catcgtcgac ggtaagctgg gacaccgcca agctgggcaa
    23701 gcgacagtcg ggcagatcaa acaccgacca ctctgcttca tagggcgccg aatcgatcca
    23761 caaaggatgc ggggcaaact cactgtgcgg attcatttca tcgggaagca gttcatcacc
    23821 gacgacaaaa ccataccgat agcggcccgg tggaagcggc aaagacaggg taaaatagcg
    23881 cgttccttga agcgaggtct gctcgtcgaa gcgccgatag ccaggccggg caaagccgtc
    23941 ccagctgccg atgagctcgg gatgttcgac ttgctcacgc gaaagggaaa tgtctttgcg
    24001 ggcaagctgc acgtcggggc gaaagcgaac cggaagcaga cagcgtgcat catagcgcgg
    24061 ccccggctcg acggaagaac agccgcaaag gaatccgcaa acagcccaaa gttgccaaaa
    24121 gcgagggcgc gccatgcgcg acggaatgta gccaaattgc aaagggtttg ggaaaagttc
    24181 cgctcgatgg cttgggtcca ccttctgtcc gcgaggcgga cccactgcgg gcgtcgcgac
    24241 tgttgcagag tccgggtcgt ttcgggcaag ctgcggctat ggacaagaga gtcaaagatg
    24301 ccgacgcggc gctctacgac gtgagcgatg gcgccacgat catgagcggg ggttttggcc
    24361 tgtgtggcaa cccggaaaac ctgattgaag cgctgctgcg acgcggaacc cgcggcctca
    24421 cgctgatttc caacaactgt ggcaccgacg gaaaaggcct gggcaagctg ctgcaaaacg
    24481 ggcaggtacg caaaatgatt gcgtcctacg tcggggaaaa caagacgttt gagcggatgt
    24541 ttctggaagg caagctcgaa gtcgagctgt gtccacaagg cacgctggcc gaacggatgc
    24601 gcgccgctgg agccgggatt cctgccttct tcacaccgac gggatttggc accgtccgcg
    24661 ccgaaggcaa agaagtccgc gtcttcccgg accgcctggg acgacagcgc agctacatcc
    24721 tggaaaccgc gctgtcggca gactatgcgt ttgtcaaagc ctggaaaggg gaccgcttcg
    24781 gcaacctcgt ctatcgcaag acggcgcgca atttcaatcc ggtgatggcc accgcagcgc
    24841 gcatcacgat tgcggaagtc gaacagatcg tcgagcctgg cgagattccg cccgatcaag
    24901 tccacacccc cggaatcttt gtgaagcgca ttttccaagg cgcaaactac gtaaagccaa
    24961 tcgagcagcg aactgtgcgg ccccgtccgt cgtccgcggt gtaaggagtt tggtgatggc
    25021 gattactcgt gaacagatgg cccagcgcgt cgccagcgag ctgcgcgatg gcatgtatgt
    25081 aaaccttggg attggcctgc cgacgctggt tgccaactac gtaccgcctg gaatggatgt
    25141 gattctgcaa tcggaaaacg gaatgctcgg aacaggtccg tttccgtttg aaggagaaga
    25201 agatccagac cttatcaacg cgggaaaaca gaccgtaaca gagctgcccg gtgcatcgta
    25261 tttttcgtcg gcagagtcgt tcggaatgat ccgcggcgcg cacatcgaca tctgcatttt
    25321 gggtgcgctg caagtgtcgc agaccggtga tctggccaac tggacgattc ccggcaagat
    25381 ggtaaaggga atgggcggtg cgatggatct cgtcgccggg gcccgtcgcg tcatcatctt
    25441 gatggaacat gtcgccaagg tcaaaggcgc agcggaagga agctttgatt tcaaagtcgt
    25501 aaaggaatgc aagctgccgc tgacggcccc gcgctgcgtc gcgcggattg tgacggatct
    25561 ggcggtgatg gatgtccgcg aagatggtct tcacctggtt gaagtggcgc cgggcacgac
    25621 ggtggaagaa gtccagcgct ataccgaacc gcagctgatt ctgccgccgg gtggagtcgt
    25681 tcccgtgatg gcggtgtagc aaaggaatcc caattcccat ggcactcctt tcgcatgcag
    25741 gacgaccggg cgcgcctcct cttgccgtcg atgcggtggt gtttggggag gccctggttg
    25801 atttttttcc cgaaaatcca ggcacaaaac tagaagatgt cgagcgcttt gtccgtcacc
    25861 tcggcggcgc tccttgcaac ctggcgattg gacttcggcg acaaggaatc cccaccgcac
    25921 tggtcacgca ggtcgggtcc gatgcgtttg gccgcttcat caagcagaaa ctggacagtg
    25981 aaggtgttgc cgtagatggc atcgcttcgc acaagagcgc acgcaccgga gtcacttttg
    26041 tttccgtcgc tgcgaccggt gaaagatcgt ttctgtttta ccgccatcca tccgcggaca
    26101 tgctggtcac aactgccgaa gtgacaccgc ctttgatctt gcgcggacgg ctatttcact
    26161 tcggatcatc gtctttgtcg cgggaaccag cgcgcgccgc aaccctgcat gcactggagc
    26221 ttgtcaacaa gcagcgcaaa cagcgcatcg tgagctgtga tctgaacctg cgcccacatc
    26281 tttggccgga cataaaggaa gcgccaccgc tattgcgcag agtcctttcc ggctgcgatg
    26341 tcgtcaagct caccgacgat gaacttccga ttctgtgtgg aacagagagc atcgaagcgg
    26401 gtgcgcagta tgttcgcggt ctgggcgcga cgatggtggt ggttacgctt ggtgatcgcg
    26461 gcgcgtactt cgattcacca caaggaactg cgtacattgc tgcggaatcg gttccggttg
    26521 tcgatcaaac aggggccggc gatgccttta ccgcgggtct tttggcgacg ctgctcgtct
    26581 atttgaacgg atcaggacca gatgatctgc gcgagcggct caaagcgctt cctttcgatg
    26641 tcttaaagcg cggctgtctg cacggaaccc acctggcggc gcgcgtctgt acggcgctcg
    26701 gagcgacaac tgccctgccc catctgccgc cgccgccgcc gccacttgtc aaagtctcgg
    26761 cttctagtaa gaaggatttc taatgcctcc tatcaagatc atcggtctgg gcggttcgct
    26821 aaaacccgaa tcagcgagtg tcaaagcact ccgcatcgct ctgtccgggg cagtggaaac
    26881 cggtgcgcac atcgagcttt tggagctgca cgaaatcgcg ctgccgatgt ttgttccgga
    26941 catggaaccg ccaccgcagg tccgccggtt tgtcgacgct gtttcaggct ctgctggcat
    27001 gatttggtcc agtcccgtct atcacggcag catgagcggt gcgttcaaaa acgcactcga
    27061 ttggctggag cttctgtcca aacatgatcc gccctatctg accgacaaag tggtgggact
    27121 tatcgcaacg gcaggaggcg tacaaggact gcaagcgata aacgcgatgg agtttgccgt
    27181 cagatcgctg cgcggctata cacatccgct gactgcgccg attgatcggg cctggcagtt
    27241 gtttgacaag agcggaaact tttccgatga agcgacgatt gcgcggctgc gtaccttggg
    27301 acaaggcgtc gccagcgcag cccgcaggct cggtttgccg caccactgct aactccgcgg
    27361 agtccccatg gtcacggtcc tgcaagagcc gatgcgtctt tatgacttcg cctggccaga
    27421 gcgcttttca tcccgcgcga gtcgacatgg cgtactctgt tattttgaca gcggcaaagc
    27481 ggacgatggc ccaccgctgg tcatgctgca tgcgctggga atcaacttca cccagtggcg
    27541 tcacgtcgcc cagatcctgg ccccgcagca gcggctgata ggaatcgaca tgccgggctg
    27601 tggacattcg gccaagccac gtcaccgctg gcaactgtcc gacatgacag aagcggtcat
    27661 cgggcttttg gatgaactgg gaatcgagcg cgccatgatc gccggacatt cgtttggcgg
    27721 acggatcgca ctggagctgg cgctgcgtca tccggagcgg gtagccgggc ttttgctgct
    27781 caatgcggcg ggcttcattc gctatcccaa gctgctatcc cacgtcggtg agcgactgct
    27841 aaaacccaag cttgttgcac cgattatggt ggcgctgtcc aagccgattc tgggcaccat
    27901 tgtcgcgcat ccaacaagtc cgtccggaga gtttgccgct tccgtcaccg aaagattccg
    27961 tccagagttt gcgtgggatt ttgcgtatca cgcttgtccg cttttgccag cgctgctgtc
    28021 cgatgtgatc gatgaactgc aacatctgca tcttccgatc gaggtgctgt ggggccgcag
    28081 agaccgcttg ctccgctatc gcagcgtcga aaaacacctg tacagaatcc cgactgcgcg
    28141 gatcgatgtg attgatgact gcgggcatat gccaaacttt gaacatcccg acctcgtcgc
    28201 caaggccgcc gaaaggctgc gttcccgtgt gaggaaagga tgaccgaagg acgtaaggtg
    28261 cgctacggcg acgagctcat ctacgacgcc gagtttacgc gcaagttcat tgcctggatc
    28321 gagccgatcg cacgggccta ctttcgcgcc gaggtacgtg gtgcagagct tttgccgcgc
    28381 aaacagacgc tgctggtcag tcaccatgat ggcggaatga tgccgattga ctctctgttt
    28441 gtcggcaaag cctggcatga gcggttttcc tttcaccgtc cgctgcatgt gctggtccat
    28501 gacatggtgc tgcgttttgc ccgtccactg cgacggatcg gcgcggtgct ggcggatcgt
    28561 cacaacctgg atcggctgat tgaaagtggt cgatcggcgc tgatttatcc gggggccgca
    28621 cacgagacat ttcgaccgtt ctgggaacgc aagatcatca cgcttgggaa tcgcaccgga
    28681 tttatcaaac atgcgctgca caagcggatt ctgctgactc cggtggtctc tgcaggtgtc
    28741 catgaaacct ttgtggtgct gtcgcgtgga tcgtggctgg cagagcgact ccgtttccgc
    28801 gagctgtttc gcgccaatgt gttcccggtg gtcgctggac tgccgttcgg aatttggctc
    28861 ggtgcgtttt ttccgcagct cccccttccc gccaaaatca cggtgcagat catgccgcct
    28921 gtcgacttgg taccgtgggc ggagcggctt ctgggtcgcg cctggaaaga cagcgacgcc
    28981 gacgaacaag atgtagtctg gacttgcttt tacgagcttc gcgacagcat gcagcgcgcg
    29041 ctcgatcggc tgtacgccga gcggcgcttt cccatcctgg gctgagcacg acgtcttctc
    29101 agaagctcgg cttggcggta tcgtaagcta tggccgaatc aggcagcgcg cctccagggg
    29161 gggggacaaa gccactggca atgaccaact tggtcgtcgt caaagcgacc ccttctgccg
    29221 atatttcgac ggacaaaacc atggccgccg gcagcgaaac tgccgagtcc gccaaagcca
    29281 cccagcgcga ccaggtcggc gcccccgtcc acctggcctg gcgcgaaccc gtccgcggcc
    29341 gcgccctggc ctggttcatc caggcctttg gcggcgtgca gtttggctaa ggcgcgggaa
    29401 ggcgcgaggg g
//
LOCUS       DAORGN010000042        28775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_620_length_28775_cov_5.837430, whole genome shotgun sequence.
ACCESSION   DAORGN010000042 DAORGN010000000
VERSION     DAORGN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28775
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_620_length_28775_cov_5.837430"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKL17_06965"
     CDS             complement(<1..410)
                     /locus_tag="PKL17_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44504.1"
                     /translation="MTTYVVMASHVEEISQIVLSPQELAAIQHGGHIEFAGLSIQVED
                     DVLRAEGEAIADVPTFKHLLRICEEIFRLHGSWALLAIGNDQTPAMSAERRRILAEWS
                     QTHPARGIALVRITNPLAITMLSLMMRAMNVLRAT"
     gene            complement(503..2035)
                     /locus_tag="PKL17_06970"
     CDS             complement(503..2035)
                     /locus_tag="PKL17_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNK44505.1"
                     /translation="MRSLFSLLAGLLRFLWALLTLLTPLLGVWLGSSLVSFYGLRAEL
                     ALAAGVLLFPILPILWELRATAAWNRARTEERRMGKPPVRWLSGLERLVLRTMIINLA
                     FLGGLLGYFPKVAFSALATRGDWFLFEKHDAASDTLRSLLLTGAGGLEWLHRLANPNP
                     YKKPGDDVPAPETVKPIDDQKPTYPIRRWRKTTDPVPSAHPPDPQPSPEPPSTVGPAP
                     TWQVGDTTWPQQNDVSSVVLDMKPSDEISIEQVARHIASKEHDPFARVKALHDWVVTR
                     LDYDHDSLRRGQRKPQDAASVFARRNAVCEGYARLLVALGQSSGDHFVYVHGDTREEG
                     GERAAIGHAWNAVEVLGKWYIVDATWDDPTIDGKSAHYRTDYLFIPPRLAIYDHRPEN
                     DKWQMLSQPLSRGEFLRQPLTRPSLSRVGLELLVPDSTRVEVNDGVQLQLANPRHLYL
                     SARIGLSDAEHSERCGVSHSDPVTLFCPTPHQGKYRATVFANHQESGTYDQVLALDVF
                     RR"
     gene            2178..3281
                     /locus_tag="PKL17_06975"
     CDS             2178..3281
                     /locus_tag="PKL17_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44506.1"
                     /translation="MTSNHSFRCSTCNSVLLFSPSQGGLRCTHCQAVEVPKKSTLAVS
                     ERDLLAQLSAHHRADGPTLAELPKSQSARCKECGATVVFPAHITARVCAFCGSPSVLP
                     PKDPTQSLRPESIIPFSVDKQKAKALMGTWARSLRFRPADLIRASQAERLIGIYIPHF
                     TFDAHVDSSWTAKAGFDYSETEEYEETVDGQTVRKTREVRKIRWEDRSGTRSDDYDDL
                     LICAGRGLPPGLADSVSNFQTRALVPYAPDYLAGFFAEDLAMGLAEGWKRGESSIRWL
                     QERRCAFDVGGDHQADLRVESSLSNLTYKAVLLPFWLATYRYRERSYRVLINGQTGRI
                     DGESPKSFAKVGLWLVLLVLAAVVLYGLWTTYF"
     gene            complement(3306..4565)
                     /gene="ftsZ"
                     /locus_tag="PKL17_06980"
     CDS             complement(3306..4565)
                     /gene="ftsZ"
                     /locus_tag="PKL17_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNK44507.1"
                     /translation="MALLEFDDNHNHAKIKVIGVGGGGGNAVSTMILSRLEGVDFIAA
                     NTDLQALESNQAQIKIPLGTKVTRGLGAGANPEIGRQSAMEDRERIAEVLQGADMVFV
                     TAGMGGGTGTGAAPVIAEVARELGALTVGVVTKPFGFEGKKRRRHAEDGIGALAAAVD
                     TLITIPNERLLSVAGQKTTMLDAFRKADDVLLNAVQGISDLITIPGLINVDFADVKTI
                     MSNMGRALMGTGRASGERRAVEAAQQAISSPLLEDVSITGATGILINITGGPDLTLFE
                     VNEASTLIQEAAHEDANIIFGSVIDPSIGDELRITVIATGFDAQYADVEPTIYDRRPS
                     TTQHQVPISFVQQKAEMAPPPPPPAARVEVAASGPARRDRVATPVTTQVRTAPVRSAI
                     GTRLSFEEEDFDTPTYQRRSQQVAARE"
     gene            complement(4688..4978)
                     /locus_tag="PKL17_06985"
     CDS             complement(4688..4978)
                     /locus_tag="PKL17_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44508.1"
                     /translation="MTRFRATGLVAALGLFTATAYINYVSLVDAYGSGPPYYGRTTNM
                     DKWSSPLWGLLMIDGVVLLMVLAAVQLFLRRQAETRALEEVPEPKLDQDSDP"
     gene            5271..5846
                     /locus_tag="PKL17_06990"
     CDS             5271..5846
                     /locus_tag="PKL17_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HNK44509.1"
                     /translation="MAVVHAYKQTDDKRTKDGDLIEVTSGWCIGMVVHWILAKKEGAD
                     FWKKAAGADGEQAFRMIMAMQQIWYKQGGWNQKKEYDIFDKLLGGKKVKRVHYNDTTP
                     KKVISPEDVWMGITNTAGRFGLINIGFAQGGHAMGVCKNSDESFNFFDPNCGEFKWKD
                     VAEFRKGLKAEIFDKMNYKNAVNRLIIAKYD"
     gene            5918..7507
                     /locus_tag="PKL17_06995"
     CDS             5918..7507
                     /locus_tag="PKL17_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44510.1"
                     /translation="MERLLFVFRMQWHKSPVFVCVLCLSWLASLLPIWIGRYVPLLDY
                     PAHLANLYIFRHLNDPALGFSQYYETNLAPLPYWVQYGIEYGLSIFVGEELAQKLFLS
                     LAIGLLPWTVALYARALGRDPWLLVLAIPLAWNMNVANGFLAYVGGLPLLFVALWSLA
                     KYSEQPSVSRALVATLLGVLLYFSHILLWGTLLCAGGLTALVCCRPFAIRRVLVALCP
                     LVPTALLGLWAQLYGDADKTRVTVRGAGLRDIQGVWSSVAQNLDMLRGWTMNTLPETR
                     DENGYQLLWLAAWLVLLIVGFRSEARTPTEKIQTVPLGSRLLGRAEVGFACAVGLFFS
                     LPRSLLRPFYWYAVNRRLAVVVALFAILLIRGSLFSSALRRGILLGVMVLSIVYTVDV
                     SAHYYRVNQRLAAFDALIAQVPRGKQVLPLMSQFGDPDCVVNCFNQWGSYLQIRQGGY
                     MIPYFPIEFPLRRKQVPTPTPIPWDHPQSFRFREHARGWDYFLIHGPVLPSFFAGSED
                     AVRLVGQRGDFTLYEKRAGLP"
     gene            complement(7513..9984)
                     /locus_tag="PKL17_07000"
     CDS             complement(7513..9984)
                     /locus_tag="PKL17_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK44511.1"
                     /translation="MTELSPDLPPLKRADLRRILEHTLRTEEIIDSFVVDSFPPEVYR
                     RIRDRTYVGKLNELLAELDTDQILRQLLHWVEIDERNEILRALRGSPVEVSRPLLCLG
                     EVFPTTGVPQYTLVDPEQLVDIVDGMRRRGMGLLVEGPSGIGKTTAVRRALQLLPADA
                     PKVIWKNGRLREDQDTLRRIVQSGLHDGGHLIVDDFHRVPADLRQDLADLIKSLSDSA
                     RQDAKVTLIGINPLGSSLIGPNTDIVGRYVTVDLRRQPAARIADLLRKGEHALNIEFR
                     QRDSFISEARGSFLTAQALCYECARKEGVVETERRRRTLVAAPGDYAVDRVQQHLAER
                     FDAGLRDFAAFDALSPPRGAALALLWMLHESSENSVSLAAASQRFPVLREAIEWLQKG
                     KLQQRFEQAGHLRELFYWNREGGVLSIEDPRLDFYLQHLNWSAFIRDTGHRRDRVFWT
                     RQRGLEIYPAENGSISGLVPPITLPDGQLPNYDLPESYVLHLSDLHFQSITQVVLWLD
                     QLEADLRDSRALAIPKLDAIIVSGDISESSSSEQFDAAEQFLWELRKSYKLPPHRLVL
                     VPGNHDVSWEYSRAAYQEIDGAQIDRDKLRGFEGAWVEEAGRFYLRDDAKYAHRFSPF
                     ADFFKRACMQAYPLEAEMQSTLHAFPQIKLLCLGLNSAWICDHQKQYRGRASIHGAAL
                     AQALRTLRQEPTFADWLKIAVWHHPTQGSEDDRIRDTGFLDRLSQAGFRIGLHGHIHS
                     AGIASHRSDLTLGGRPLDLLGAGTFGAPTKEWRSGVPLQYQLLRIKGNRLTVYSRCKD
                     NPEGVWRADGRWGMGKAASEYEIEL"
     gene            10084..11085
                     /locus_tag="PKL17_07005"
     CDS             10084..11085
                     /locus_tag="PKL17_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44512.1"
                     /translation="MKRELLLCAVASLLGCSSGGATSTNPDPSPDLALAPGNDDMAVS
                     PGGADMAQVNDMLPPPDLTPPIPSEADCFTDWKKLGNCPAPQITESYLGNNCAGTTGV
                     FVVGRYFQSGNKFWTTNGFMPYGPYALASKLGRDTWNYLTPRIMCITTSNDATYWTGF
                     AMQVKNPDGVTSNSVTVTNKVGTTPALPSTGSTSAFDWNACMDAPMTYTQGQAKIVPP
                     ASSATLGPVTISKRTRPCNAVSGCGDWNPTTTETTTTAGLQVVGGTSIHFMLGTTDCG
                     AIGSGFVSTNYCSTTGPAGSYDAHVSANCMMLNRRGFSSVAGDGSYTETHWGAVIRY"
     gene            11117..11626
                     /locus_tag="PKL17_07010"
     CDS             11117..11626
                     /locus_tag="PKL17_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44513.1"
                     /translation="MGKSGKSKPTFRISDTGAYVCPHCQTQNQYAMAECRVCGAPPPQ
                     LQAQRVRQKQLGDIGSGWMEVGTLFIGFGVAESLFLGVHSVRRRDDEVALGALVLFFA
                     LAYCIDPARRRSPGAFVTAALLTVSLSVVVFALGFWLHALFPFGSSCALFAAARQAMR
                     REGILSQKL"
     gene            11648..12355
                     /locus_tag="PKL17_07015"
     CDS             11648..12355
                     /locus_tag="PKL17_07015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44514.1"
                     /translation="MKNTQTSAHATAAEPQVRRRNLRTWVSDSLHDLFFEVIALPGKT
                     AQKFILDRVFAWKYRVHDPWSLRSSAYEQTRYQRMLDLLPDRAYPRVLELGCAEGAFT
                     HKLADAIAGAQILGVDISDKAIARAQQICQDQSQVTLRVMNVLSGLPQGPFDLVFCSE
                     VLYYLDRSQRKQLARKLAHAMAPGALLVSSNIFPRAQFLHRELSRIPSLKLLTEQLFV
                     DEPPGYALALYQRTVAQ"
     gene            12368..13111
                     /locus_tag="PKL17_07020"
     CDS             12368..13111
                     /locus_tag="PKL17_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44515.1"
                     /translation="MNRTRLLGLLGLCLVFVLAGLWIATQGDLLLGLGCAIMFGGGVA
                     IFGLQILDSYREQEAALIDGEVRLPPSVPIHESPLRPRIAMAVMGCFGLGAVLVGIDR
                     QPLLLLIGAALCVLTLVFPFFKAKGYLTSRIMFSPQGIHIIERKRRYLIPFEHIVKMQ
                     SAEWNGHLLICIWPTTLDAVVDTVEPADQKESLHKDLLKSEAWMGTPLTLWPTRFGMQ
                     GELLLRALHRYISDPSSRQELKPQAALAP"
     gene            complement(13136..13702)
                     /locus_tag="PKL17_07025"
     CDS             complement(13136..13702)
                     /locus_tag="PKL17_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK44516.1"
                     /translation="MALHFQHKQVLSAELSALPHTSPRLPLIFRFGTWEDLQKQKGHP
                     DYGYGAIPYLEGRLRAGDSFLLGELDGQLVMTAWLMRGTMELGELIVPIPKDHAYHYK
                     LYTIEKFRRQGAARGLWMFLRERLMEQGIVRMISTVERRNTTSLHAHVQAGFRPLGSF
                     AELQVGPMSGYVLSRALRRYLRDQRLPG"
     gene            13933..16302
                     /locus_tag="PKL17_07030"
     CDS             13933..16302
                     /locus_tag="PKL17_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK44517.1"
                     /translation="MRGRDLLCSAAIVWSVGTSARATVDTVIRGSVRDELLAPVASAD
                     VRLYDRRGRLLRSTTTDAQGQFSFAGVAFGEYEVRAKAEGRAETKQRVQPSSAEAAEV
                     DLFCVAQNTVAVIVEEREAQAPSRAVGTVATLTRETLKALPKGEDRPITEVVTTQPGF
                     VQDAFGNVYSRGNHSNIQYQIDGIPIPDSVGSLFAQSIPVRLIDSLEVLTGGLGAEYG
                     NRLAAVVNINTRRGGNTLDGMVQLRYGSFETLESSGFLSYGRGPFSFFVGGSYMQSQR
                     MLDPPAPGTSLHNFGQNARLFARLDYTAGEHDRIELFANYAQNRFQIPINQSLKPYDA
                     SLPDGGRTPDEYGNDPAPYVPHDTSASETEHEVFLTLSWIHSFGKKGQLQIAPYYKLS
                     FGALSSDPEHALGELADPGMVVSDVTRRADHGGAVAHYSVLIGRHMLKVGAQLNYLRG
                     ATDFTKYTRDDENPQGGIALAQPGVDRTSALLTGAYIQDRFEFGRLGLQYGVRFDLQH
                     VILADGQTSDQWGVSPRLGASFAFLKELVGHAFVGVLFTPPPPLDLGNAARVLGVIPP
                     DEQVPYDIKAETAAYGELGLSGRLWKKLKLSVTGWGKYSWNQLDNIALGSTNLIGAYN
                     FEHGRTIGVELAADFVFHEHLSAFANATWLVAQGQGISSAKFLFDAEQLADTSWQTLD
                     HAQSYTANAGLTVSYQGAQATALMNYGSGLRTGPTNNQSVPGHVRFDLTLQYSFDHLP
                     VRPRLAIDIINLFDAQYAYRIGNAFVGSSYAAPRSVFARLAIPLDFPTR"
     gene            complement(16330..17592)
                     /locus_tag="PKL17_07035"
     CDS             complement(16330..17592)
                     /locus_tag="PKL17_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44518.1"
                     /translation="MQSQTEQLARKRRSLSLPGHNLRTHGEPSWADRLGDALLIPGIP
                     EQIPPQDRQVVEAALGPHLFHSYAGRTHPLLVRLLCQDMQPGESLLDPFVGSGTVLVE
                     GVRIGLKTVGCDISPLSIRLSRLKATPMPEPMQKALLLRAQSVCDASLARVKKRLSPP
                     RNYDDPQFYAPHVYLELCGLRQEIASVCADDAPLGEALLLIFSSIVVKASRQRSESSQ
                     DLVERQIGKGQVSRWFLRKAEEFVRLQSAFWQKGQKTIPPLLIEGDARSELLAQTEQK
                     LARGSVQRVITSPPYLGTYDYLHHHLRRYAWLGIDPAPIERDEMAARRQALPLRKRIE
                     RHEADTWNWLSAVERLLQTGGLCFVLVGDSVVDETLLDGAQPIHRAAESTRLRVMAEA
                     SVERPHAVRTVHPLPPRREHLILLQRNG"
     gene            17749..18465
                     /locus_tag="PKL17_07040"
     CDS             17749..18465
                     /locus_tag="PKL17_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNK44519.1"
                     /translation="MATRETGQHYQDPKKALWCLKKLPLLADIPHDTLSQLADSVSLL
                     DLRRRQVIYLPGDPGGAVYFLSSGRVKISKVTRDGKELTLDYRVAGDVFGELCLIEGG
                     AREDMAEAMDQTLVIEVERGQFERLVQKEGLIGFRLVKVLAQRRRELEGKIENLIFKD
                     VNSKLAELLLRLGQDYGIDDSRGTLVSLKITHQEMANLIGSTRETVSLTLSQFKRSGL
                     IATDGRRVILSNQEGLRALA"
     gene            complement(18490..20022)
                     /locus_tag="PKL17_07045"
     CDS             complement(18490..20022)
                     /locus_tag="PKL17_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44520.1"
                     /translation="MTLAVLGLLLLFLLRAIHFLQVMNKDDQRSEEGQQDLKLSLKLC
                     GWLLFAGSLVYALESLRHGEVYWLILPLLLLSLLWRPMFIVLDVLVPRGLAKWTYRTA
                     MLLLWASADRRGQALLYANLALLRAPSHRDVRDDEAFLLEKQKQLPGGAAALLAAALQ
                     VARREGIQSIVPLLRCLDSVDPTVKPNWAMAVAHEILLVDAVRIGDWKKVQTLSLEPF
                     ETPLSQFLAACADIFVGLPTKDTTKARDRLYWLWLRAPKKQHLLWLLEQAQKYDPQKP
                     GSAGQMDPVKMTADRPQIEVALMLKAWLLQTDPHQVKAEDLRGLGAVWDLALADQGLD
                     RLIMTRALTLGIAGSEQKAKDTLRSQVVATLRSYLSQGTMPIAKLFPSGKLPPKSVLS
                     DAVLQVRAELLEQFEAQTNKLEERTQDKRSLPWLDEWREWAQIRESYQKIEDFGGMEA
                     RRLAWTSLHRQANNWACWLWNARSEKVLANAVFRFLLRESDALGDERTSQLAKKNVGS
                     GL"
     gene            complement(20019..20369)
                     /locus_tag="PKL17_07050"
     CDS             complement(20019..20369)
                     /locus_tag="PKL17_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44521.1"
                     /translation="MSRERFLENPFYVLGVRPECSRAEMEREGQKLLGMLEIGLPSAK
                     KYKTPLGEAERTPEMVRQAMAELRDPQRRLLHELWAQLPAERVAITPNGSDGGSGPGP
                     WPDAVSALGFGAKK"
     gene            complement(20366..21235)
                     /locus_tag="PKL17_07055"
     CDS             complement(20366..21235)
                     /locus_tag="PKL17_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44522.1"
                     /translation="MAATMQLARRLSSGEGDASAEERALVAIDTHGAVISPLRRYAGR
                     AAVYGALSILSGIGVCSLLSGDPLVLALSLVGTAAWGHGLLVTRWLEQASQLIHLSRL
                     DEAEQLLLRCLRPPWGSETVRGHAHLRLSTLASHRGSHSEALEQARMAAALFAGEYPP
                     QPQFLQLSRYQEVRALLSLGKLADARHLFAELGGAPSGDYLRAHYYVTELYLALAEGQ
                     IPFLDEPLWERAQLALDTDHAPPLLGLCAWGFDRLGDEETAQHFRKLCQKRCGEQLPL
                     TLPLLARYLARWT"
     gene            complement(21245..22288)
                     /locus_tag="PKL17_07060"
     CDS             complement(21245..22288)
                     /locus_tag="PKL17_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44523.1"
                     /translation="MPKQSDAPSADAVAPERVGTSLARTASEDRSLDRQEMPDALLSA
                     VDHALDRPPRDVPTMQLAKTLEEAGPRTFVYIDSQGRVRPPTQYRMMQAASYAMLTTI
                     LVGGSVLYAYLWGALGLLFPLVFGSLVGRNLLITRRINQAALLSSHDRLDEAEAILRR
                     LLRRRGLGRRLRALVHHNLGAVATRRGDHKEAVTQLREAIALYKDAWRKSPHLRSCQY
                     GEVIALCNLGKVDEARERLAQVHITDQGDYLLLKYWTTELYVRFCAGDEPPSAVELWE
                     RAERALKITASSALLALCAWAYTKGKVPDHDMAWHLLRESIDRLDNEPLARIMPPLWK
                     WIMQNRTAAESAA"
     gene            22392..23009
                     /locus_tag="PKL17_07065"
     CDS             22392..23009
                     /locus_tag="PKL17_07065"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNK44524.1"
                     /translation="MQSTNWSSGQLSAYAVAGLVTCSLVGYFAWALPDMLHRGRDGIR
                     KTREAPCQVLRPSPTSAALGRLPQPAPDFTLKTHDGQDVKLSSLRGRVVLLNFWATWC
                     PTCSVEEPSLERLATELKGQPFSLLAVSVDDNWDVVRQHFSSGSAMTVLLDKERKVPQ
                     SYGTEKFPESFLIDRDGNVRYFVVSERQLWHTQEVKACIQALLDE"
     gene            23080..23796
                     /locus_tag="PKL17_07070"
     CDS             23080..23796
                     /locus_tag="PKL17_07070"
                     /inference="COORDINATES: protein motif:HMM:NF028481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNK44525.1"
                     /translation="MVKLYGFGAGLGLPDPSPFVVKVAHYLRMIAVAYEPCAGNARKA
                     PKGKLPYIEDSGAVVADSSFILDHLRKKYQDLDAGMNAQDRALAIAVKALLEEQLYFV
                     MVTQRWKEDRGFAVLQKGLAEILRKAGLPSFAISTVAGLIRKQVIKTVNAQGMGRHSE
                     QEVEDIGIASLESLSELLGDKKNFLGDAPRSIDATVFAFLWLILAAPYENAVKAYAQS
                     KTNLVAYTERHKTQYWSDWK"
     gene            complement(23816..25198)
                     /locus_tag="PKL17_07075"
     CDS             complement(23816..25198)
                     /locus_tag="PKL17_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44526.1"
                     /translation="MTIRAGLAACLVIGLASCGSKEVPLLPEEISGHCNYVNKFSSEP
                     ECRNYHGDWPADKIMEDCADWNGTADIGTPCSAQSVLGQCILEKDGLYIAAQAIGDNP
                     DKCASTKRGCELFGGGVFDAAPICGGVESSGSDGVGLPVFQQPVRVCKDPVAGEAAGQ
                     SAGGQVCTWEMISGVTEEGRHFEDYASCDRVRTQRPYYGAGTLPNNTREDPRLKDPTY
                     ATEQAWVMSQIKSAACVCCHSTKAPKGPSNWYVDQPGNFLNGFYDRGLAMGAGWLDTV
                     GFGAYPPSENNGFSRATPDNPGHSIFPTQDDARMRRFFEKELAARGKTRADFAGEKYV
                     AGPLDEQRFFRPAACTRGEGVRSDGLLVWKGGNARYVYVLAASAASPTVPPNLDLPQG
                     TLWRIDVPTDGKPVQSGTVKYGSVPAGLSQKFPASGAAPAQLVSGQQYYLYALADILQ
                     PVTRCLFTAP"
     gene            25650..26867
                     /locus_tag="PKL17_07080"
     CDS             25650..26867
                     /locus_tag="PKL17_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK44527.1"
                     /translation="MRKASFLVALMLGGAVTTLTGCPEPETGTPEVPVDMTPAKAFHL
                     TGVVVGTADQPLSNATIRVGTNAVTASGPDGKFSIDMAAPSGPVSLTISAAGYIPLVQ
                     MLPSDAMDVKYRLQRVAETSFPTSAFALTVADPASGAVVSIPADGLQGPGGAAPDGEI
                     TISIRFIDPSKQPMPGGDGALGTKNEQLALDPYGIVYTAARDSKGNVLSLKSGVTMTL
                     YIPVPNDRLAMAPSEVGLWKLDSTKNAWVQDIGPSGTPTMLTRKNTSFTCRDKQLDPC
                     DILACSNVSNQAWVGQASSFGFLNADVSFDKPACLRLDVQQPQQVCLHFEIPSAGSIK
                     IKEHCYGQGSFALYNLPANMPISVKTMTPGAACSPDLSMGKMANPGDVWGGTGVPSSP
                     SLCKGSITIPPTP"
     gene            26922..28487
                     /locus_tag="PKL17_07085"
     CDS             26922..28487
                     /locus_tag="PKL17_07085"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNK44528.1"
                     /translation="MSSHPPHPTDPVERLHAAQARAEKGGAHKYHEKNAAEGKLFCRE
                     RLRLLFDPESEFVEDGLLANNQAHDLPADGVVTGIGRVHGRPVAVMANDSTIKAGSWG
                     ARTVEKIIRIQETAGRLRIPLVYLVDSAGARITDQIDMFPGRRHAGRIFNNQVQLSGS
                     IPQLCLLFGPSAAGGAYIPAFCDVVFMVSGNASMYLGSPRMAEMVIGEKTTLEEMGGA
                     KMHCEVSGCGDLLAKTEAEVIAKCRDYLSYLPSHAGGALPPPKEGASLSPRPDARRID
                     DILPKNHNQSCDIVQVIEALVDQGSFFEIKKLFAKEIVTGFARLAGRTIGIVASQPKH
                     KGGVLFVDSADKAARFIWLCDAFDIPLLFLADVPGFMIGTAVERQGIIRHGAKMISAV
                     SDATVPKICVVLRKAYGAGLYAMCGPGFHPDATIALPQSSIAVMGPEAAVNAVFFNKI
                     QEKPEAERAAYIEQLRAEYREDIDIYKLAGDLHIDAIVEGDKLRSELIRRFAFAEKKK
                     VPTYPRKRSVTPV"
BASE COUNT         5496 a         8713 c         8569 g         5997 t
ORIGIN      
        1 gtcgcgcgaa gtacgttcat cgctcgcatc atcagcgaca acatcgtgat cgcaagtggg
       61 ttggtgatgc gaaccagcgc aatgccgcgg gctgggtggg tctggctcca ttcagccaag
      121 atccgtcttc gttccgccga catcgctgga gtctgatcgt tgccaatcgc cagcagcgcc
      181 cagctcccgt gcaggcggaa gatttcctcg cagatgcgca gaaggtgttt gaaggtcggc
      241 acatcggcga tggcttcccc ttcggcacga agcacgtcat cttccacttg gatggaaagt
      301 ccggcgaact cgatgtggcc gccgtgttgg attgctgcga gctcttgcgg cgaaaggacg
      361 atctgactga tctcttccac atgcgaggcc atcacaacgt atgttgtcat ggcgcgtgcg
      421 tcttgtcact ccttgtgcgg agtttgcggc caggtgcgcg aaaccgccct ggggtggggt
      481 gatcggacgc cgcgggcgac ggtcagcggc ggaatacgtc gagcgccaag acttgatcgt
      541 aagttcctga ctcttggtgg ttggcaaaca ccgtcgcgcg gtacttgcct tggtgtggcg
      601 taggacaaaa aagcgtcaca ggatcactgt gtgaaacgcc gcagcgctcg ctgtgttctg
      661 cgtcggacag cccgatgcgg gccgacagat aaagatggcg cggattggca agctgaagct
      721 gtacgccgtc gttcacttcg acacgggttg aatcgggcac aagcagttcc agaccgacgc
      781 gcgaaagact cgggcgcgtc agtggttgac ggagaaattc accccgcgac aagggctgcg
      841 aaagcatctg ccatttgtca ttttccggac ggtggtcgta gatagcgagc cgcggcggaa
      901 taaacagata gtcggtgcgg taatgggcgc ttttgccatc tatggttgga tcgtcccagg
      961 ttgcgtcgac gatgtaccac ttgcccaaga cttcgacggc gttccaggca tgtccgatgg
     1021 ctgctcgctc gccgccttct tcgcgtgtgt caccgtggac atagacaaag tgatcacctg
     1081 agctctgccc gagcgcgacc aggagccgtg cgtagccttc acacactgca tttcgccgcg
     1141 caaacaccga tgccgcatcc tgcggtttgc gctggccgcg tcgcagcgag tcgtgatcat
     1201 agtcaagtcg ggtcaccacc cagtcgtgca gcgctttgac gcgcgcaaac ggatcatgct
     1261 ctttgctggc gatgtggcgg gcgacttgct cgatgctgat ttcgtcgctt ggcttcatgt
     1321 ccaaaacgac gctgctgaca tcgttttgtt gtggccacgt cgtgtcaccc acctgccagg
     1381 ttggcgcagg gccgaccgtg gatggtggct cgggtgatgg ctgcggatcg ggcgggtgcg
     1441 ctgacggtac cggatcagtc gttttccgcc agcgacgaat cggataggtc ggtttttggt
     1501 cgtcgatggg cttgacggtt tccggcgcgg gtacgtcgtc gccaggcttt ttgtacgggt
     1561 ttggattcgc cagtcgatgc agccattcca gtccgcccgc gccagtcagc agcaacgagc
     1621 gcagcgtgtc cgacgccgca tcgtgctttt caaacagaaa ccaatcgccg cgtgttgcca
     1681 gcgcggaaaa tgcaactttg ggaaaatagc cgagcagtcc tccgagaaac gccaggttga
     1741 tgatcatcgt gcggagcacc aggcgttcca accccgaaag ccagcgcacg ggcggctttc
     1801 ccattcgtcg ttcttcggtc cgcgcgcgat tccaagctgc cgtcgcccgc agttcccaca
     1861 ggatcggcag aatcggaaac agcagcacgc ccgctgccag cgcaagctcg gcacgcagcc
     1921 cataaaacga caccagagac gaacccagcc acacgcccaa aagcggcgtg agcagcgtga
     1981 gcagcgccca aagaaaccgc agaagtccgg ccaaaagcga aaacagagac cgcatcttct
     2041 gtcagttcga ccgagccgca gtgcgctggc aagcagtttt tcggtggtct ttccggctgg
     2101 gccttgttcc atgggaactt tggccaaatc cctgggcagg cggtcttgct catgcgaggc
     2161 ttgctgggtc gcccaccatg accagcaatc actcgtttcg ctgctcgact tgcaacagcg
     2221 tcttgctgtt tagtccaagc caaggcggtc tgcgctgcac ccactgccag gccgtggagg
     2281 tgccaaagaa aagtacgctg gcggtgtccg agcgcgatct tcttgcgcag ctttccgccc
     2341 atcatcgcgc cgatggtccg acgctggccg agctgccgaa aagccagtca gcccgctgca
     2401 aagagtgtgg tgccaccgtc gtgtttcccg cgcacatcac cgcgcgggtc tgtgcgtttt
     2461 gcggttcgcc aagtgtgctt ccgccaaaag atccgaccca gagcctgcgg cccgaatcaa
     2521 tcatcccgtt ttctgtcgac aaacagaaag ccaaagcgct gatgggaaca tgggcgcgca
     2581 gcttgcgctt tcgtcccgcg gatctgattc gcgcaagcca agccgagcgg cttatcggaa
     2641 tctacattcc ccatttcact ttcgatgcgc acgtcgattc gagctggacg gccaaggccg
     2701 gttttgacta ctccgagacc gaagaatacg aagaaaccgt cgatggccag accgtgcgca
     2761 agacccgcga agtgcgcaag attcgctggg aagaccgcag tggcacccgc agtgatgatt
     2821 acgacgactt gctgatctgc gccggacggg gacttccgcc aggactggcc gattcggtca
     2881 gcaatttcca aacgcgcgcg ctggtgccgt atgcccctga ttatctcgct ggtttttttg
     2941 ccgaagacct ggcgatgggg cttgccgaag gctggaagcg cggcgagtcg tcgattcgct
     3001 ggctgcaaga gcgacgctgc gcattcgatg tgggcggcga tcaccaagcc gacctgcgcg
     3061 tcgaaagctc gctgtcgaac ctgacctaca aagctgtgct gctgccgttt tggcttgcga
     3121 cctatcgtta ccgcgagcgc tcgtatcgag tcctgataaa cggacagacg ggtcgcatcg
     3181 acggagaatc ccccaaatcg tttgccaaag tggggctttg gctcgtgctt ttggtgctgg
     3241 ctgccgtcgt cctgtatggg ctctggacga cgtactttta ggtgggaaaa gaagcgcggg
     3301 aaagactatt cgcgggctgc gacttgctgg ctgcggcgct ggtaggtcgg cgtatcgaag
     3361 tcttcctctt cgaagctgag tcgggtgccg atcgccgatc gcaccggggc ggtacgaact
     3421 tgggtggtga ctggtgtcgc aacgcgatcc cggcgggccg gaccagaagc cgcaacttcc
     3481 acgcgcgccg cgggaggagg aggcggcggc gccatttcgg ctttctgctg cacaaacgag
     3541 atcggcactt gatgctgcgt ggtcgacgga cgacgatcgt aaatcgtcgg ctcgacatcg
     3601 gcgtactgcg cgtcgaaacc ggtggcaatg accgtgatgc gcagctcatc gccaatcgac
     3661 ggatcaatca ccgaaccgaa gatgatgttg gcgtcttcgt gcgcggcttc ttggatcagc
     3721 gtcgatgctt cgttgacttc aaacagcgtc agatccggac cgcccgtgat gttgatcagg
     3781 atgccggtgg ctccggtgat cgacacatct tcgagcagcg gcgagctaat cgcttgctgt
     3841 gcggcttcca ccgcgcgacg ttcgcctgat gcccgacccg ttcccatgag cgcgcggccc
     3901 atgttcgaca tgatggtctt gacgtcggca aagtcgacgt tgataagacc cggaatcgta
     3961 atcaagtccg atatgccttg caccgcgttg agcagcacat cgtctgcttt gcggaacgca
     4021 tcaagcatcg tcgttttctg acccgccacc gaaagcagtc gctcattcgg aatcgtgatc
     4081 agcgtatcga ccgccgccgc cagcgcgccg atgccatctt cggcatgacg acgccgtttt
     4141 ttgccttcaa aaccgaacgg cttggtcacc acaccaacgg tgagagcgcc cagctcgcgc
     4201 gcgacttcgg caatgacggg ggcagctcca gtcccggttc cgccgcccat gcccgcggtc
     4261 acaaagacca tgtccgcgcc ttgcagcact tcggcaatgc gctcacggtc ttccatcgcc
     4321 gactgccgac cgatttcggg attggcaccg gcgccgagcc cgcgcgtcac cttggtgccg
     4381 agcggaattt tgatttgcgc ttggttgctt tccagcgcct gaaggtcggt gttggcggcg
     4441 ataaaatcca cgccttcgag ccgactgagg atcatggtgc tgacggcgtt gccgccgccg
     4501 ccgcccacgc caatcacttt gatcttggcg tggttgtggt tgtcgtcgaa ttccagtagg
     4561 gccatacggt gttcctcccc gtggaattga ttgtttaagg gcgggatcta tgggtcaatt
     4621 ttttcccaat tttttccgaa aaaatatttt gcgcctcacc cgatctcggt ggatctccgt
     4681 cgctgtccta gggatcgctg tcctgatcga gtttgggctc tgggacttcc tcaagcgcgc
     4741 gcgtttcggc ctggcggcgc aaaaacagct gcacggcggc cagcaccatc agcagcacca
     4801 cgccatctat catcagaagc ccccaaagcg gcgatgacca tttgtccatg ttggtcgtgc
     4861 gaccgtagta cggcggtcca ctgccatacg catcgacgag cgacacatag tttatgtacg
     4921 ccgtcgctgt aaaaagtccg agcgccgcca caagtcccgt cgcacgaaat cgcgtcatgc
     4981 cgcttcactc atcgtggcgc gatccgtagc agtgcgcttg gtcgatcgca aaaaagtcga
     5041 tcacacaggc tggtctgctg cggcttaggt ttccgcggtt tatcggatcg caaaaaagcg
     5101 ctctatactt ttggtgcgag cgcctttccg ttgctcgcgg gggcggacac tcggtccgcg
     5161 caggctctgg ccttctgcct tgcgagcggt gggagtgctt tggttctgca gacgtggact
     5221 tggcttccat cgcgtttttg gggcggtgac tttcacagga gcaaaacacg atggctgtgg
     5281 ttcatgcgta caaacaaacc gacgacaagc gaaccaaaga cggcgacttg atcgaagtga
     5341 ccagcggttg gtgcatcggc atggtcgttc actggatctt ggccaagaag gaaggcgccg
     5401 acttttggaa aaaagcggca ggtgcagacg gtgagcaagc cttccggatg atcatggcga
     5461 tgcagcagat ttggtacaag cagggcggct ggaaccagaa aaaagagtac gacatcttcg
     5521 acaagctgct cggcggcaag aaagtgaagc gcgttcacta taacgacacc accccaaaga
     5581 aagtcatcag cccagaagac gtttggatgg gcatcacgaa cactgcgggc cggttcggac
     5641 ttatcaacat cggctttgcg cagggtggcc acgcgatggg tgtttgcaaa aacagcgacg
     5701 aaagctttaa cttttttgat cccaactgcg gcgagttcaa gtggaaagat gtcgccgagt
     5761 tccgcaaagg actcaaggcc gaaatcttcg acaagatgaa ctacaaaaat gcggtcaatc
     5821 ggctgatcat cgccaagtac gactagtcac ggtgtgcgct gcgctcgcgc ggcggtttca
     5881 gtcgctccat cgtcggtttt gtgtgtagga tgcggccatg gagcgactcc tttttgtgtt
     5941 ccgcatgcag tggcacaagt cgcccgtttt cgtctgcgtt ctgtgtctgt cgtggcttgc
     6001 gtcgcttctg ccgatttgga ttgggcgcta tgttccgctc ctcgactatc cggcgcacct
     6061 ggccaacctg tacatctttc ggcatctgaa cgatccggcg cttggttttt cgcagtacta
     6121 cgaaaccaac ctggcaccgc tgccgtattg ggtgcagtac ggaatcgagt acggactgtc
     6181 gattttcgtc ggtgaagagc tggcgcagaa gctgtttctt tcgctcgcga ttggtctatt
     6241 gccttggacg gttgcgctct atgccagggc gcttgggcgt gacccgtggc ttctggtgct
     6301 ggcgattccg ctcgcgtgga acatgaatgt cgcgaacggt tttttggcgt atgtcggcgg
     6361 tctgccgctt ctgtttgtcg cgctctggtc gcttgccaaa tacagcgagc agccgtctgt
     6421 ttcccgcgcc cttgtggcga cgctgctggg cgttctgctc tacttttcgc acatcctttt
     6481 gtggggcacg ctgctgtgcg cgggcggact caccgcgctg gtctgctgtc gaccgtttgc
     6541 gattcggcgc gtgttggtcg cactgtgtcc gctcgttcca accgctctct tgggtctgtg
     6601 ggcgcagctt tatggcgacg ccgacaagac gcgcgtgacg gtgcgtggcg cgggcttgcg
     6661 cgatatccaa ggcgtgtgga gcagcgtcgc gcaaaacctc gacatgctgc gcggctggac
     6721 gatgaacacg ctgcccgaaa cgcgcgatga aaacggctac cagcttttgt ggcttgcggc
     6781 gtggcttgtg ctgttgatcg ttggttttcg cagtgaagcg cgaacgccaa cggaaaagat
     6841 ccagactgtg ccgcttgggt cgcggctgct cggtcgcgcc gaagtgggtt ttgcttgtgc
     6901 ggtgggtctt ttcttttcgc tgccgcgcag tctgctgcgg cctttttatt ggtatgcagt
     6961 gaaccggcgg ctcgcggtgg tcgtggcgct gtttgcgatt ctgctgattc gcggttcgct
     7021 gttttcttct gcgctgcggc ggggaatttt gctcggtgtg atggtacttt ccatcgtcta
     7081 tacagtcgac gtaagcgcgc attattaccg cgtaaaccag cggctcgccg cctttgatgc
     7141 gctgattgcg caggtgccgc gcggcaaaca agtcctgccg ctcatgtcgc agtttggtga
     7201 tccggactgc gtggtcaact gcttcaacca atggggaagc tatctgcaga ttcgccaagg
     7261 cggctacatg attccctatt tcccgattga atttccgctc cgccgcaagc aagtgccgac
     7321 gccgaccccg attccttggg atcatccaca gtcgtttcgc tttcgcgaac atgcacgcgg
     7381 ctgggattac tttctgattc acgggccggt gctgccgtcg ttttttgccg gttccgaaga
     7441 tgcggttcgg ctcgttggtc agcgcggcga cttcacactg tatgaaaagc gtgcgggtct
     7501 accgtaaccg actcatagct cgatttcgta ttccgaagcc gccttgccca tgccccatct
     7561 gccgtcggcg cgccagactc cttcggggtt gtccttgcag cgcgaataca ccgtcagccg
     7621 attccctttg atgcgcaaga gctgatattg cagcggcact ccgcttcgcc actctttggt
     7681 cggtgcgcca aaggttccgg ccccgagcag atcgagcggc ctgcctccca atgtcaaatc
     7741 actgcggtgc gaagcaatcc cggcgctgtg aatgtgtccg tgcagcccga tgcgaaaacc
     7801 cgcttggctc aacctgtcga ggaatccggt gtcacggatg cggtcgtctt cgctcccttg
     7861 ggtcgggtgg tgccagacgg caatcttcag ccaatcagca aacgtcggtt cttgccgcag
     7921 ggttcgcagc gcttgcgcaa gtgctgcgcc gtggatgctc gcgcgtccgc ggtactgttt
     7981 ttgatgatca cagatccacg cagagtttag tcccagacac aacagcttga tctgcgggaa
     8041 tgcgtgcagc gtcgactgca tctcggcttc cagcggatag gcctgcatgc aagcgcgctt
     8101 gaaaaagtcg gcaaacggcg aaaagcggtg cgcgtacttg gcatcatcac gcagataaaa
     8161 gcggcccgct tcttcgaccc acgcgccttc aaaaccacgc agcttgtcgc gatcgatctg
     8221 tgcaccgtcg atttcctggt acgccgcgcg cgaatattcc cagcttacat cgtgatttcc
     8281 gggcaccagc accaggcggt gcggcggcag tttgtaactt ttgcgcagct cccacagaaa
     8341 ctgctcggcg gcgtcgaatt gttcgctgct ggagctttcg ctgatgtctc ccgacacaat
     8401 gatggcgtcg agcttgggaa tcgccagcgc tcggctgtcg cgcagatccg cttccagttg
     8461 gtcaagccaa agcaccacct gcgtgatgct ctgaaagtgc agatccgaca gatgcaggac
     8521 atacgattcc ggcaggtcgt agttcggaag ttgtccgtcg ggtagtgtga tcggcggcac
     8581 cagtcctgaa atgctgccat tttccgccgg ataaatttcc agtccgcgct gtcgagtcca
     8641 gaacacccgg tcgcggcgat ggccggtgtc acgaatgaac gccgaccagt tgaggtgttg
     8701 cagatagaaa tcgagccgcg gatcttctat cgacagaacc ccgccttcgc gattccagta
     8761 gaacagctcg cgcagatgtc ctgcttgttc aaaacgctgc tgaagcttgc ctttttgcag
     8821 ccattcaatt gcttcgcgca gaacgggaaa acgctggctg gctgctgcaa gggatacgct
     8881 gttttccgac gattcatgca gcatccacaa aagcgccagc gccgcgccgc gcggtggtga
     8941 cagcgcatca aacgcggcaa agtcacgcag tcccgcatcg aagcgttcgg ccaggtgttg
     9001 ctgcacccga tcgaccgcgt aatcccccgg cgctgcgacc agtgtgcggc ggcggcgctc
     9061 ggtttcgacg acaccttctt tgcgcgcgca ctcataacag agcgcttgtg ccgtcagaaa
     9121 gctgccgcgc gcttccgaaa tgaagctgtc gcgctgccga aattcgatgt tgagcgcgtg
     9181 ctcccctttg cgcagcagat ccgcaatacg tgccgctggc tgccggcgca aatcgacggt
     9241 gacataacgc ccaacgatgt cggtattggg cccaatcagc gacgaaccga gcggattgat
     9301 tccgatcagc gtcactttgg catcttgccg cgcgctgtcc gagagcgact tgatcaggtc
     9361 ggccaagtct tgtcgcaggt ctgccggcac ccggtgaaag tcatcgacaa tcaggtgtcc
     9421 gccgtcgtgt agaccgcttt ggacaatgcg ccgcagcgtg tcttgatctt cgcgcagccg
     9481 accatttttc caaatcacct tgggggcgtc cgctggcaaa agctgtagcg cgcgtcgcac
     9541 cgccgtcgtc ttgccaattc cggatggacc ttccaccaga agacccatcc cgcgacgccg
     9601 catgccatca acaatgtcga cgagctgttc cggatcgacc agtgtgtact gcggcactcc
     9661 agtcgtgggg aagacttcgc ccaaacacag cagcggacgg gacacttcga cgggactgcc
     9721 acgcagtgcg cgcaggattt catttcgttc gtcgatttcg acccagtgca gaagctgtcg
     9781 caggatttga tcggtatcca gttcggcgag cagctcgttg agcttgccga cataggtccg
     9841 atcgcgaatc cgccgataca cttccggggg aaagctatcg acgacaaacg aatcgatgat
     9901 ctcttcggtg cgcagcgtgt gttccaagat gcgccgaaga tcggcacgct tgagaggggg
     9961 caaatccggt gaaagttctg tcatcgtttc tgccgcgctg tgcgtgatgt gattcccact
    10021 ttagcactcg gacgtatcga ccgggagggt ctcgaacctt tcgcagtgat accattgcca
    10081 gtcatgaaac gagaactgct tctttgtgcg gttgcttcgc tgcttggttg ctcgtcgggc
    10141 ggtgcgactt cgacaaatcc agatcccagt ccggatctcg cgctcgcacc tggcaacgac
    10201 gacatggcgg tctcacctgg gggcgcggac atggcccagg tgaacgacat gctgccgccg
    10261 cctgatctga caccgccgat tcccagcgaa gccgactgtt ttaccgattg gaagaaactg
    10321 ggaaactgtc cagcacccca gatcaccgaa tcgtatttgg gcaacaactg cgccggcacc
    10381 accggtgtgt tcgtggtcgg tcgctacttt cagtctggca acaagttttg gaccaccaac
    10441 ggctttatgc cgtatggacc gtatgcgctg gccagcaagc ttggtcgcga cacctggaac
    10501 tacctgacgc cgcggatcat gtgcatcacc accagcaacg acgccaccta ctggactggc
    10561 ttcgcgatgc aggtcaaaaa ccccgatgga gtgacttcca attcggtcac ggtgacaaac
    10621 aaagtgggca caacgccagc gcttccttcg acgggaagca ccagtgcatt tgactggaat
    10681 gcctgcatgg atgcgccgat gacgtatacg cagggtcagg ccaaaattgt gccacccgct
    10741 tcgagtgcca cgctcgggcc tgtcaccatc agcaagcgga caagaccctg caatgcggtg
    10801 agcggctgcg gcgactggaa tcccaccacg accgaaacga cgacgaccgc agggctgcaa
    10861 gtggtcggcg gcacttcgat acactttatg ctcggaacca ccgactgtgg cgccatcgga
    10921 tcggggtttg tcagcaccaa ctactgctcg accaccggcc cggcgggttc ttacgacgcg
    10981 cacgtttcgg caaactgcat gatgctcaac cggcgcggct tttcgtccgt cgctggcgat
    11041 ggaagctaca ccgaaaccca ctggggcgca gtgattcgtt actaagccag cttgcgtgcg
    11101 ggggggcgct cgctcgatgg ggaagtccgg caagtccaag ccaacatttc gcatcagcga
    11161 tactggggcc tatgtctgtc cgcactgtca gacccagaac cagtacgcga tggccgaatg
    11221 ccgggtctgt ggcgcaccac ccccgcagct gcaagcgcag cgcgttcgtc aaaagcagct
    11281 tggtgacatt ggttccggct ggatggaagt ggggacgctt ttcattggtt ttggcgtcgc
    11341 ggaatccctt ttcctggggg tgcacagcgt ccgtcgccgt gacgatgagg ttgcacttgg
    11401 cgcgctcgtt ctttttttcg cgttggctta ctgcatcgat ccggcgcgtc ggcgaagccc
    11461 tggcgctttt gtgacagccg ccctgctgac cgtttctctt tccgtggtgg tttttgcgct
    11521 tggcttttgg ctgcacgcgc tgtttccgtt tgggtcaagc tgcgcgcttt ttgcggcggc
    11581 ccgccaagcg atgcgacgcg aaggaatttt gtcccaaaag ctttgatatt cattgcaaaa
    11641 atgcggcatg aaaaacaccc aaacgtcggc ccacgcgacc gcagccgagc cgcaagtacg
    11701 tcggagaaac ctacggacct gggtgtctga ttcgctgcac gatctgttct ttgaagtcat
    11761 tgcgctgccc ggaaagaccg cccagaagtt catcttggac cgggtatttg catggaagta
    11821 tcgcgtccac gatccgtggt cactgcgctc cagcgcctat gaacagacgc gctatcagag
    11881 aatgctcgat ctgcttcccg atcgcgccta tccgcgggtg ctggaactgg gctgtgccga
    11941 aggtgctttc acccacaaac tggccgacgc catcgcgggg gcccagatcc tcggcgtcga
    12001 catcagcgac aaagccatcg cgcgcgccca gcaaatctgc caagaccaaa gccaagtcac
    12061 gctgcgggtg atgaacgtgc tgtccggtct gccgcagggc ccctttgacc tcgtgttctg
    12121 tagcgaagtg ctctactacc tcgatcgttc ccagcggaaa cagcttgcac ggaagcttgc
    12181 ccacgcaatg gctccggggg cactgcttgt ttcatccaac attttcccgc gggcgcagtt
    12241 tctgcatcga gagctgtcgc gcattccatc gctcaagctg cttaccgaac agctctttgt
    12301 cgacgaaccg ccgggctatg cgctcgcgct ctatcagcgc actgtggcgc agtgataggc
    12361 tctgtggatg aaccggacgc ggctgcttgg tcttctcggt ctgtgccttg tgtttgtgct
    12421 tgccgggctg tggattgcga cccaaggtga tctgctgctt gggctgggct gcgctatcat
    12481 gttcggcggt ggcgtggcga tttttggact tcagatcctg gattcctatc gcgaacagga
    12541 agccgcactc atcgacggag aagtccgctt gccgccatcg gtgccgatcc acgaatcgcc
    12601 gctgcgtccg cgcatcgcga tggccgtcat gggctgcttt ggtctgggcg ccgtgctcgt
    12661 cggaatcgat cggcaacccc tgcttctgct gattggcgcg gcgctgtgtg tgttgacgtt
    12721 ggtgttcccg tttttcaaag ccaaaggcta tctcacgtcg cggatcatgt tcagtccgca
    12781 ggggattcac atcatcgaac gcaagcgccg ctatctgatt ccgtttgaac acatcgtcaa
    12841 gatgcagtcc gcagagtgga acggacatct tctgatttgc atctggccga cgacgctcga
    12901 tgctgtggtc gataccgtcg agcccgccga tcaaaaggaa tccctgcaca aagacctgct
    12961 caagtcggaa gcctggatgg gaactccgct gacgctgtgg ccgactcgtt tcggaatgca
    13021 gggggaactg ctgcttcgcg cgctgcatcg ctacatttcc gacccttcca gtcgtcagga
    13081 actcaagccg caagcggcac ttgcaccgta gccaaagacg ccgcggtctt cgctcctagc
    13141 ccggcaagcg ctggtcgcgc agatagcggc gcagcgcgcg cgacagaaca tagccagaca
    13201 tcgggccaac ctgtagctct gcaaacgaac cgagcggacg aaaacccgct tgaacatgcg
    13261 catgcagcga cgtggtgttg cgccgttcca ccgtacttat catccgcaca atgccctgtt
    13321 ccataagccg ctcgcgcagg aacatccaca gaccacgcgc tgcaccttgg cggcggaact
    13381 tctcgatggt gtagagcttg tagtgatagg cgtggtcttt gggaatcggc acgatcagct
    13441 ccccaagctc catcgttccg cgcatcagcc acgccgtcat cacaagctgt ccgtcaagct
    13501 ctccgagtaa aaaggaatcg cccgcgcgca gccggccttc caaataagga atcgcgccat
    13561 agccgtagtc gggatgccct ttttgttttt gcaaatcctc ccaagtgccg aagcgaaaaa
    13621 tcagcgggag ccgcggcgag gtatggggta gggcggacag ctctgccgag aggacttgtt
    13681 tgtgctgaaa gtggagtgcc atggcgggct cctggtgtgc ttgtgccatg acacatttcc
    13741 gctgcgaacg caaagtgacg tgccgggtgt gggggtctgt cggctgcacg accgcacttg
    13801 cacttaatcg caagttactt gcaaaaagaa acagccccag gataggctga ttcagtcgtt
    13861 tgggccttct gcccagatgg attttgcaag caagtggcat cttcggtttg acttggggag
    13921 gaaagacaga ggatgagggg acgcgatctt ctgtgcagtg cagcgatcgt ttggtcagtc
    13981 ggcacttctg cccgtgcgac tgtggacacg gtgattcgtg gatcggtgcg agacgagctg
    14041 cttgcgcccg tcgcttctgc cgatgttcgg ctgtacgatc gacgcggcag gcttcttaga
    14101 agcaccacca ccgatgcgca aggccagttt tcgtttgctg gggtggcgtt cggtgaatac
    14161 gaggtgcgcg ccaaagccga aggccgggcg gaaaccaagc agcgggtgca gcccagctcc
    14221 gccgaagccg cggaagtcga tctgttctgc gtcgcccaaa acaccgtcgc agtaatcgtc
    14281 gaagaacggg aagcgcaagc tccttcacgc gcggtgggaa cggtggcgac gctgacccgc
    14341 gaaacgctca aggcgctgcc caaaggcgaa gatcgtccga tcactgaagt ggtgacgacg
    14401 cagccgggct ttgtccaaga tgcgtttggg aatgtctatt cgcgcgggaa tcactcgaac
    14461 attcagtatc aaatcgatgg gattccgatt cccgattccg tgggaagtct gtttgcgcag
    14521 tcgattccgg tgcggctcat tgattcgctg gaagtgctga ctggcggtct gggcgccgag
    14581 tatggcaatc ggctggctgc cgtcgtcaat atcaatacca ggcggggcgg aaacacgctg
    14641 gatggcatgg tgcagcttcg ctacggctcg tttgaaacgc tggaatcgag cggttttttg
    14701 tcctatggcc gcggcccgtt ttcattcttt gtcggcggca gttacatgca gtcgcagcgc
    14761 atgctcgacc cgccggctcc ggggacttct ctgcacaact ttggtcaaaa tgcgcggctg
    14821 tttgcgcggc tcgactacac cgccggggaa catgatcgga tcgagctgtt tgcaaactat
    14881 gcgcagaacc gctttcagat tccgataaac cagagcctaa aaccctacga tgccagcttg
    14941 cccgacggtg gacggacccc cgatgaatat ggcaacgatc cggctcccta cgttccacac
    15001 gacaccagcg ccagcgagac cgaacacgaa gtgtttttga ccctgtcgtg gattcattcg
    15061 tttgggaaaa aaggccagct tcagattgcg ccgtattaca agctgtcgtt tggcgcgctg
    15121 tcatcggacc cagaacatgc gctcggggaa ctggccgatc cgggcatggt ggtcagcgat
    15181 gtgacgcggc gcgcggatca cggcggagcg gtcgcgcatt attcggtgct gatcgggcga
    15241 cacatgctga aggtgggcgc gcagctaaac tacctgcgcg gtgcgaccga ttttacaaag
    15301 tacacccgcg acgacgaaaa cccccaaggt ggcatcgcgc tggcgcagcc tggcgtggat
    15361 cgcacgtcgg cgctgctcac cggcgcatac atccaagacc gctttgaatt tggccggctc
    15421 ggtcttcagt acggtgttcg ctttgatctt caacatgtga tcctcgcaga cggtcagacc
    15481 agtgatcagt ggggcgtaag tccgcggctt ggcgcgtcgt ttgccttttt gaaagagctg
    15541 gtcggtcacg cctttgtcgg tgtgctgttt acgccgccgc cgccgctcga tctgggcaat
    15601 gccgcgcgcg tgctcggcgt cattccgccc gatgagcaag ttccctacga catcaaggcc
    15661 gaaaccgccg cgtatggaga gcttggacta tcgggccggc tgtggaaaaa gctcaagctg
    15721 tcggtgacgg gctggggcaa gtattcctgg aatcagctcg acaacattgc gctcggatcg
    15781 accaacctga ttggtgccta caactttgaa catggccgca ccattggcgt cgagctggcc
    15841 gctgactttg tgttccatga acacctgtcc gcgtttgcca atgcgacctg gcttgtggcc
    15901 caagggcagg gcatttcgtc cgccaagttt ctgtttgacg ccgagcagct tgccgatacg
    15961 tcgtggcaga cgctcgatca tgcgcagtcc tataccgcca atgccgggtt gaccgtcagt
    16021 taccaggggg cgcaggcgac ggcgcttatg aactatggtt ccggactgcg gaccgggccg
    16081 accaacaacc agagtgtgcc ggggcatgtc cgcttcgatc tcacgctcca gtattcgttt
    16141 gatcatctgc cggtgcgacc gcgtctggcc atcgacatca tcaacttgtt tgatgcgcag
    16201 tatgcctatc ggatcggcaa tgcgtttgtc ggcagctctt atgcggcccc ccgatcggtg
    16261 ttcgcgcgct tggcgatacc gctcgatttt ccgacccggt aagcccggcg ttcacagcgc
    16321 tcgggtcgac tacccgttcc gctggagcaa gatcaggtgt tcgcggcggg gcggaagagg
    16381 atgcacggtc cgcaccgcat gtggacgttc gaccgacgct tcggccatca cccgcagtcg
    16441 agtggactcg gcggcgcgat ggatcggctg cgccccatca agcagcgtct catccaccac
    16501 cgaatcaccg accaggacaa agcacagacc gccggtttga agcagccgct cgaccgccga
    16561 aagccagttc caggtatcgg cttcgtgtcg ttcgatccgt ttgcgcaaag gcagcgcctg
    16621 tctgcgggcg gccatttcat cgcgctcaat cggtgccgga tcgatcccaa gccacgcata
    16681 gcgtcgcaag tgatggtgca gatagtcata cgttcccagg tacggcggcg acgtaatcac
    16741 ccgctgcacc gaaccgcgcg caagcttttg ctcggtctgc gccagaagct cgctgcgcgc
    16801 atcgccttcg atcaaaagcg gcgggatcgt cttttgtccc ttttgccaaa acgcgctctg
    16861 taagcggaca aactcttcgg ccttgcgcaa aaaccagcgc gacacctggc ctttgccgat
    16921 ctgtcgctca accagatcct ggctcgattc gctgcgctgt ctggatgcct tcaccacgat
    16981 cgaagaaaag atcaaaagca gcgcttcccc aagcggcgca tcgtctgcac agaccgacgc
    17041 gatttcctgc cgcagaccgc acagctccaa atagacgtgc ggcgcgtaga actgcggatc
    17101 gtcgtagttg cgcggtggcg acagccgttt tttcacccgc gcaagcgatg cgtcacagac
    17161 gctctgcgcg cgcaggagca gcgccttttg catgggctct ggcatcggcg tcgctttgag
    17221 ccgcgacagc cgaatcgaca gcgggcttat gtcacagccc accgttttta gaccgattcg
    17281 cacgccttcc acaagcaccg ttcccgaccc gacaaatgga tcgagcagtg actcacccgg
    17341 ctgcatgtcc tgacacaaaa gccgcacgag caaaggatgg gttcgcccgg cgtagctgtg
    17401 aaacagatgt ggacccagcg cggcttccac cacctgtctg tcttgtggcg gaatttgctc
    17461 gggtatcccc ggaatcagca gcgcgtcacc caatcggtcg gcccacgaag gctccccatg
    17521 ggtgcgcaag ttgtgaccgg gcaaagacag agatcgccgc ttgcgggcaa gctgttcagt
    17581 ctggctttgc atcagcgttc tcccccgaaa acatccactg caagacgggc gcgcggcccg
    17641 gttgatagcg accccagctg tgtagaaagc ctcacgaagc gggaatcaaa cgtaatcggc
    17701 aaaaccgatg gttgccgggc cagggtcgtt tgcagtatgt aggggcgcat ggcgactcgc
    17761 gaaaccgggc aacattatca ggatccaaaa aaagcgctgt ggtgtttaaa gaagctgccg
    17821 cttctggcgg acattccgca cgacaccctt tcgcagcttg cagacagcgt ctcgcttttg
    17881 gatctgcgtc ggcgacaggt gatttatttg ccgggcgatc cgggcggcgc ggtgtacttc
    17941 cttagctcgg gccgggtcaa gatcagcaag gtgacgcgcg atggcaaaga gctgacgctc
    18001 gactaccgcg tggccggcga tgtctttggc gagctttgtc tgattgaagg cggcgcccgc
    18061 gaagacatgg ccgaagcgat ggaccagacg ctcgtgattg aagtcgagcg gggacagttt
    18121 gaacgcctgg tgcagaagga aggactcatc ggcttccgtc tcgtcaaggt gctggcccaa
    18181 agacggcgcg agctcgaagg caagatcgaa aacctgatct tcaaggacgt aaactccaag
    18241 ctggccgagc tgcttctgcg cctgggccaa gactacggca ttgatgacag ccgcggcacg
    18301 ctggtgtcgc tcaagatcac ccaccaagag atggccaacc tcatcgggtc cacccgcgag
    18361 acggtatcgc tgacgctcag ccagtttaag cgcagcggcc tgattgccac cgatggacgt
    18421 cgcgtcatcc tgtcaaacca agaaggactt cgcgcactcg cgtaagcgcc gcccgcgctg
    18481 tcggcgttct tacagaccag agccgacgtt ctttttggcc agctgcgacg tgcgttcatc
    18541 cccgagcgcg tccgactccc gcagcaggaa acgaaacacc gcattggcca ggactttttc
    18601 gctgcgcgca ttccacagcc agcaggccca gttgttcgcc tgccgatgca gtgatgtcca
    18661 cgccagccgc cgcgcttcca ttccgccaaa gtcttcgatc ttttgatacg actcgcggat
    18721 ctgcgcccac tcccgccact cgtcaagcca aggcagcgac cgcttgtcct gcgtgcgttc
    18781 ttcgagcttg tttgtctgcg cttcaaactg ttccaaaagc tcggcgcgaa cttgcagcac
    18841 cgcatcgctg agcacgctct ttggcggtag ctttccactt ggaaacagct tggcaatcgg
    18901 catcgtgcct tggctcaaat agctgcggag cgttgccacc acttggctgc gcagtgtgtc
    18961 tttggctttc tgttcactgc ccgcaatgcc cagcgtcagc gcccgcgtca tgatcagtcg
    19021 atccagcccc tgatccgcca gcgccagatc ccacacggcc cccagcccac gcaggtcttc
    19081 tgctttgacc tgatgcggat cggtctgcaa aagccacgcc ttgagcatga gcgccacttc
    19141 gatttggggg cgatccgccg tcattttcac agggtccatc tgtcccgcgc tgccgggctt
    19201 ttgtggatcg tacttttgcg cctgttcgag cagccacaac agatgctgtt tctttggcgc
    19261 ccgcagccac agccaataca gcctatcacg agccttggtc gtatctttcg tcggaagacc
    19321 gacgaaaata tcagcgcaag cggcaaggaa ctgcgacagc ggtgtctcaa acggttcaag
    19381 cgacagcgtc tgcacttttt tccagtcgcc gattcgcacc gcatcgacca gcagaatttc
    19441 atgcgcgacc gccatcgccc agtttggctt gaccgtcgga tcgaccgagt ccaagcagcg
    19501 cagaagcggc acaatcgact ggattccctc gcggcgagcc acttgcaacg ctgccgccaa
    19561 cagcgccgcc gccccccctg gaagctgctt ttgtttttcc agcagaaacg cttcgtcgtc
    19621 gcggacatcg cggtggctcg gcgcgcgcaa aagcgccaag ttggcataca gcagcgcttg
    19681 tccgcgtcga tcggcagacg cccacaaaag cagcatcgcc gtccgatacg tccacttggc
    19741 caagccccgt ggcacaagca cgtccagcac gatgaacatg ggccgccaca gcagcgaaag
    19801 cagcaacaat ggcaggatca gccagtacac ctcgccgtga cgcagcgact ccagcgcgta
    19861 gaccagcgat cctgcaaaca gcagccagcc gcacagcttg agcgacagct tgaggtcttg
    19921 ttggccttcc tcgctgcgct ggtcgtcttt gttcatcact tgcagaaagt gaatcgcgcg
    19981 cagaagaaaa agcagcagca ggcccaggac ggcgagcgtc acttcttggc tccgaagccg
    20041 agcgcactga ctgcatctgg ccacggacct ggaccgctgc caccgtcgct accatttggc
    20101 gtgatggcga cccgctcggc gggcagttgg gcccacagct cgtgcaaaag ccgccgctgc
    20161 ggatcgcgca gctctgccat cgcttggcga accatttccg gggttcgctc ggcttcgcca
    20221 agtggcgtct tgtacttttt ggcgctcggc agaccgattt ccagcatccc gagcagcttc
    20281 tgcccttcgc gctccatttc ggcgcggctg cactccgggc ggaccccgag cacataaaac
    20341 ggattttcga ggaagcgctc tcggctcatg tccagcgtgc gagatagcgt gccaaaagcg
    20401 gcagcgtcaa ggggagctgc tcaccacagc gcttttgaca cagcttgcgg aagtgctgcg
    20461 ccgtttcttc gtcgcccagc cgatcgaaac cccaggcgca cagtcccaga agcggcggcg
    20521 cgtgatcggt atcgagcgcc agctgcgccc gctcccacag cggctcatcc aaaaagggga
    20581 tctgaccttc cgcaagtgcc aagtacagct cggtgacgta gtagtgggcc cgcaggtaat
    20641 cgccgcttgg cgcgccaccc agctcggcga acaggtggcg cgcatcggcc agcttgccca
    20701 gcgacagaag cgcccgcact tcctggtagc gcgacagctg caaaaactgc ggctgcggcg
    20761 ggtattcgcc agcaaacagc gcggcggcca ttcgcgcctg ttccagcgct tcgctgtggc
    20821 tgccgcggtg gcttgccagt gtcgaaagtc gcaggtgggc gtgcccgcgg accgtctctg
    20881 aaccccacgg cggacgcagg cagcgcaaaa gaagctgctc tgcttcatcc agtcgcgaca
    20941 ggtggataag ctgcgacgct tgctctagcc agcgcgtcac cagcaggccg tgtccccacg
    21001 ccgccgtgcc caccaagcta agcgccagca ccagcggatc tcccgataga agagagcaca
    21061 cgccaatgcc cgacaggatc gacagtgcgc cataaacggc ggcccgcccc gcgtagcgcc
    21121 gaagcggcga gatcaccgcg ccgtgcgtat caatggccac cagcgcccgc tcttccgcgg
    21181 acgcatcccc ctctcccgat gacagcctgc gcgcgagctg catggttgcc gccatcaatc
    21241 ccccttacgc tgccgactcc gcggcggtgc ggttctgcat gatccacttc cacaggggcg
    21301 gcatgatgcg ggcgagcggc tcgttatcga gtcgatcgat ggactcccgc aacagatgcc
    21361 aagccatgtc gtgatcgggc actttgcctt tggtataggc ccaggcacac agggccaaaa
    21421 gcgccgatga agctgtgatt ttaagcgccc gttccgctcg ctcccacagc tcgactgccg
    21481 atggcggttc atcgccggcg cagaagcgaa cgtaaagctc cgtcgtccag tacttcaaaa
    21541 gcaggtagtc gccctgatcc gtgatgtgca cttgggccag gcgctcgcgc gcttcgtcga
    21601 ctttgccaag gttgcacagc gctatcacct cgccgtactg acacgagcgc aggtgcggac
    21661 ttttgcgcca ggcgtctttg tagagcgcaa tcgcctcgcg aagctgggtc acggcttctt
    21721 tgtgatcgcc acgccgggtc gcaaccgcgc ccaggttgtg gtgaaccagc gctcgcagcc
    21781 gtcgcccaag tccccgacgg cgcagaagtc ggcgcaagat cgcttccgct tcatcgagcc
    21841 tgtcgtgaga tgacaaaagt gccgcctggt tgatccgcct tgtgatcagc aagttccgac
    21901 cgaccagcga gccgaacacc agcggaaaca gaagcccaag cgcgccccac aggtaggcat
    21961 aaagtacgct tccaccaacg aggatggtgg tcagcatggc atacgacgcg gcctgcatca
    22021 tgcggtactg cgtcggtggg cgcacccggc cttggctgtc gatatagaca aatgttcgcg
    22081 ggcccgcttc ttccagcgtc ttggcaagct gcatcgtcgg cacgtcccgc ggcgggcgat
    22141 caagcgcgtg gtcgacggca gaaagcagcg cgtcgggcat ctcttgccga tcaagcgagc
    22201 gatcctcgga cgccgtgcgg gcaaggcttg ttccgacccg ctcaggcgct accgcatcgg
    22261 cactcggtgc atcgctttgc tttggcactg tgtctagcct atcacgacat gcgcagactt
    22321 ttcgccgctg cttgttgccg caaagtgcaa gctcaagtac cctcggctgc cggaagatgg
    22381 tgacaaaccg catgcaatcc accaactggt cttcgggaca gctctcggcc tacgccgtcg
    22441 ccgggcttgt cacttgctcg ctcgtcggtt acttcgcgtg ggcactgccc gatatgctcc
    22501 accgcggtcg cgacggcatt cgcaaaaccc gcgaggcacc gtgccaagtg ctgcggcctt
    22561 cgcccaccag tgccgcgctg ggccggctgc cgcagcccgc gcctgacttt accctgaaga
    22621 cccacgacgg tcaggatgtc aagctctcct cgctgcgtgg gcgggtggtg ctgcttaact
    22681 tttgggcgac gtggtgccca acttgttcgg ttgaagaacc ttcgctcgaa cggctggcca
    22741 cagaactcaa aggccagccc ttttcgctgc ttgcggtcag cgttgatgac aactgggatg
    22801 tggtgcggca gcatttctca agcggatcgg cgatgaccgt gctgctcgac aaagagcgca
    22861 aggtgccgca gtcctacggt acggaaaagt tccccgagtc gtttctcatc gaccgcgatg
    22921 gcaacgtccg ctacttcgtg gtcagcgagc gtcagctttg gcacacccaa gaggtcaaag
    22981 cctgcatcca agcgctgctc gacgaatagc gccggcaggc tttttcagcc gtccactttt
    23041 gtctggcttc gcagcgtggt cttaagtaag tctgcgccca tggtgaagct ttacggtttt
    23101 ggggcgggtc tgggactgcc ggacccgagt ccgttcgtcg tcaaggtggc gcactacctg
    23161 cggatgattg ccgtcgcata tgaaccctgt gccggcaatg cgcgcaaagc ccccaaaggc
    23221 aagctgcctt atatcgaaga cagcggtgcc gtagtggccg actcttcgtt catcctcgac
    23281 cacctgcgga agaagtacca ggatctggat gcagggatga acgcccagga ccgcgcgctc
    23341 gccatcgcgg tcaaagcgct tttggaagag cagctttact ttgtgatggt gacgcagcgc
    23401 tggaaagaag atcgtggctt cgccgtgctg caaaaaggac tggccgaaat cctgcgcaaa
    23461 gcgggcctgc ccagcttcgc gatatcaacg gtggcggggc tgattcgcaa gcaggtcata
    23521 aagaccgtca acgcgcaggg aatggggcgg cattccgagc aggaagtgga agacatcggc
    23581 atcgcgtcgc tggaatcgct gtctgagctg ctcggtgaca agaaaaactt cctgggcgat
    23641 gcgccgcgtt cgattgacgc gacggtgttt gctttcctgt ggcttatctt ggccgcaccg
    23701 tatgaaaacg cggtcaaagc ctacgcgcaa agcaagacca acctggtcgc gtacaccgag
    23761 cgtcacaaga cccaatactg gtcggattgg aagtaggggg gaaaacgcgg ttgcgctacg
    23821 gggcggtaaa cagacagcgc gtgaccggct gaagaatgtc cgcgagcgcg tacaggtagt
    23881 actgctggcc gctgacaagc tgcgccggag cggccccgct ggctgggaac ttctgcgaaa
    23941 gtcccgcggg tacgctgccg tatttcacgg ttccgctttg gaccggcttg ccatcggtag
    24001 ggacatcaat gcgccacagc gttccctgcg gcagatcgag gttcggcggc accgtcgggg
    24061 aagctgcgct ggccgcaagc acgtagacat agcgcgcgtt gcctcctttc cagaccagga
    24121 gtccatcgga ccggacacct tccccgcggg tgcacgctgc gggccggaag aaccgctgct
    24181 catcgagcgg gccagccacg tacttttccc cggcgaagtc ggcacgggtt ttcccgcgcg
    24241 ccgccagttc cttttcaaaa aagcgccgca tccgcgcatc atcctgggtc gggaagatgc
    24301 tgtgccccgg attgtctggg gtcgcgcggg aaaacccgtt gttttccgat ggtggatacg
    24361 caccaaatcc caccgtatcg agccagccgg cgcccattgc cagaccccga tcataaaagc
    24421 cgttcaggaa gttgcccggc tgatcaacgt accagttcga cgggcctttg ggcgctttgg
    24481 tcgaatgaca gcagacacac gccgcggact tgatctgcga catcacccag gcctgctcgg
    24541 tcgcatacgt cgggtctttt agtcgtggat cttcgcgcgt gttgtttggg agtgtgcccg
    24601 ctccgtaata cggacgctgg gtgcgcaccc ggtcgcagct tgcgtagtct tcaaagtggc
    24661 gaccttcttc ggtcacgccc gagatcattt cccacgtaca gacctggccc cctgcgctct
    24721 gtcctgcggc ttccccagct accggatctt tgcagacccg aaccggctgc tgaaacacgg
    24781 ggagtcctac gccatcgctg ccgctcgact ccacgccacc gcagatcggg gccgcatcaa
    24841 atacgccacc accaaacaat tcgcagcccc gcttggtcga tgcgcacttg tctggattgt
    24901 ctcctatcgc ttgtgccgcg atgtaaagac catccttttc caagatgcac tgacccagga
    24961 cgctctgtgc actgcacggc gtaccgatgt cggcggtgcc gttccagtct gcgcagtctt
    25021 ccatgatttt gtcggcgggc cagtcaccat ggtagttgcg gcactctggc tcgctgctga
    25081 acttgtttac gtagttgcag tggccggaga tttcttctgg gagcaggggc acttccttgc
    25141 tgccacagct tgctaagccg atgacaagac acgccgcaag accggcacgg attgtcattt
    25201 ggttccagtt tgggaatcgc tgtgaacacc acacagatgc agtctgacca gatcggaaac
    25261 tgggcttcat cattgccgtc gctccttggg caagtccgga ccccgcatgc cccttgggct
    25321 gttcaccgat tgcgcagggc acatcgaatg ggatgctgct tggcggcaaa ctgtgtcacg
    25381 tcaccagctg cgtttttcct cgcgcgtgat gtggcttccg gtgtggccag agggggccca
    25441 tctcactacg ttccgcgcgc cccctttggg ccaagccaag tccgcagccg gcagatcctt
    25501 ctctgctgcg taagtagcag taactacatt gcgattgcct gcccccccat gtaaaaattg
    25561 gaaccggcgt tgctcatttt ccgttgtcac cctctgcgga tagagcctgc tacggaccac
    25621 acaagaccta atcgttggag agaaaagtca tgcggaaagc ttcgtttctt gttgcgctca
    25681 tgttgggtgg tgcggtgacc accctgactg gatgtcctga gccggaaacc gggacgcctg
    25741 aagttccggt tgatatgact ccggccaaag cgttccacct gaccggcgtt gtggtcggca
    25801 cggctgacca gccactatcg aatgccacca tccgcgtcgg tacaaacgcg gtaacggcca
    25861 gcggccctga cggaaagttc tccatcgata tggccgctcc atcggggccg gtgtcgctca
    25921 ccatcagcgc tgcgggctac atcccgctgg tccagatgct gccctccgat gcgatggacg
    25981 tgaagtatcg cttgcagcgg gttgcagaga cttcattccc gacgagtgcc tttgcactca
    26041 cggtcgcaga tccagccagc ggtgccgttg tttcaattcc tgccgatgga ctccagggcc
    26101 ctggcggcgc ggccccagat ggcgaaatta cgatctcgat ccgcttcatc gatcccagca
    26161 agcagccgat gccgggtggc gatggcgcac tgggtaccaa aaacgaacag cttgcgctgg
    26221 acccctacgg catcgtctat accgccgcgc gcgacagcaa gggcaacgtg ctctcgctca
    26281 agtcgggtgt gacgatgacg ctgtacattc cggtcccgaa cgaccggctg gcgatggcac
    26341 cgagcgaagt ggggctgtgg aagctggatt cgacgaaaaa cgcctgggtg caagacattg
    26401 ggccaagcgg tacgccgacc atgctgaccc gcaagaacac cagcttcacc tgccgcgaca
    26461 agcagcttga tccctgcgac atcctggcgt gcagcaacgt gtcgaaccag gcgtgggttg
    26521 gtcaggcatc gtcgtttggc ttcctaaacg ccgatgtctc gttcgacaaa ccggcctgcc
    26581 tgcggcttga tgtgcagcag ccgcagcaag tctgcctcca cttcgagatt ccgtcggcag
    26641 gatcgatcaa gatcaaggaa cactgctacg gtcagggcag ctttgcgctc tacaacttgc
    26701 cggcgaacat gccgatttcc gtcaagacca tgacgcccgg tgctgcatgc agtcccgatc
    26761 tgtcgatggg caagatggca aaccccggcg atgtgtgggg cggcactggc gttcccagca
    26821 gccccagcct gtgcaaaggt tccatcacga ttccgcccac gccgtaacga ttccgcttcg
    26881 caagcagccc cgcgtcacag gttcgtgtaa tatgcggggc catgtccagc catcctcccc
    26941 atccgactga tccggtcgaa agacttcacg ctgcacaggc ccgcgccgaa aagggcggtg
    27001 cgcacaagta tcacgaaaaa aacgctgccg agggcaagct gttctgtcgt gagcgactgc
    27061 ggcttctgtt tgacccagaa tcggagtttg tcgaagacgg tctgctcgcc aacaaccagg
    27121 cccatgatct tccagcggac ggtgtggtca ctggcattgg gcgtgtgcat gggcgtccgg
    27181 tcgccgtgat ggccaacgac tcgacgatca aagctggatc gtggggcgcg cggactgtcg
    27241 aaaagatcat tcggattcag gaaaccgccg gtcggctgcg cattccgctt gtgtacttgg
    27301 tggacagcgc gggcgctcgg atcaccgacc agatcgatat gtttcctggc cgacgccacg
    27361 cgggccgcat cttcaacaac caagtgcagc tttcgggatc gattccccag ctctgtctgc
    27421 tgtttggtcc gtcggcagcc ggtggcgcgt acatcccggc attctgcgat gtggtgttca
    27481 tggtcagcgg caacgccagc atgtacctgg gatcgccgcg gatggccgaa atggtgattg
    27541 gcgaaaaaac caccctcgaa gagatgggcg gcgccaagat gcactgcgaa gtgtcaggct
    27601 gcggcgactt gcttgccaag accgaagcgg aagtcatcgc caagtgtcgc gactatctgt
    27661 cctatctgcc ttcgcatgcg gggggcgcgc tgccaccacc caaagagggg gcttcgcttt
    27721 cgccgcgtcc ggacgcacgc cgcatcgacg acattttgcc caaaaaccac aaccagtcgt
    27781 gcgacatcgt tcaggtgatc gaagccctgg tcgatcaggg cagcttcttt gaaatcaaaa
    27841 agctgttcgc caaagaaatc gtcactggct ttgctcggct tgcgggccga acgatcggca
    27901 ttgtggcgtc gcagcccaaa cacaaagggg gcgtgctgtt cgtcgattcc gctgacaaag
    27961 cggcgcgctt tatctggctg tgcgatgcgt ttgacattcc gcttttgttt ctggcggacg
    28021 tgcccggttt catgattggc accgcagtcg aacgacaagg catcatccgg cacggggcca
    28081 agatgatttc cgcggtgtcc gatgccaccg tgccaaagat ttgtgtggtt ttgcgcaagg
    28141 cgtatggcgc agggctctat gcgatgtgtg gtccgggttt tcatcccgac gcgacgatcg
    28201 cgctgcccca atcttcgatt gcggtcatgg gaccagaagc cgccgtcaac gcggtcttct
    28261 tcaacaagat ccaagaaaag cccgaagcag agcgcgctgc atacatcgaa cagctacgcg
    28321 cggagtatcg cgaagacatc gacatctaca agctggctgg tgatcttcac atcgacgcaa
    28381 ttgtcgaagg cgacaagctc cgcagtgagc tgattcgtcg ctttgcgttt gctgagaaaa
    28441 agaaagtccc cacctatccg cgcaagcggt ctgtcacccc ggtgtagtgt ccctgtggcc
    28501 tacgcgccgg ctccttctgt gggccgggcg gccacgtccc ccaaaaacgc tggcgcagag
    28561 ctgggccccg gttggacatc gtttctgctt ggtcacaatc ggtgggccgt gctgcgggcg
    28621 cggtgaatgt tgccgcgtgc gcgatggttc cgcgttcgca gcctgcttgg ttttggtttt
    28681 tcgatgtgtc cgctgctcgg acatgggaaa aaacgcgact tgggaatgcg gccagacttc
    28741 cggaccgcgg ctgtttcgcg aatccgtagg tggcc
//
LOCUS       DAORGN010000043        28768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_621_length_28768_cov_5.341692, whole genome shotgun sequence.
ACCESSION   DAORGN010000043 DAORGN010000000
VERSION     DAORGN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28768
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_28768_cov_5.341692"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKL17_07090"
     CDS             <1..878
                     /locus_tag="PKL17_07090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNK44529.1"
                     /translation="ANCAVQGASAPPQIVQAIERLAPHVDVMIVGRGGGSVADLWCFN
                     DEQVARAIHRCPVPVVSAVGHEVDFTIADFVADVRAPTPSAAAELCVPEHARLFRELG
                     ELSRRLLRAGRRLVDGGRQQIDQLLGRGQMAIERNLMQKRRTLGDLGKRLTACHPKTQ
                     LLRDQGQIDKLHSRLVAAMEKQTSERRQNLLSLTGSDDERLVRAVQRLLATKRQQLVG
                     EVGKLEALSPLKVLTRGFAVARDAEGRVLRDSAAVTDGDSISVQLAQGALFCTVDSRS
                     LPRSQNFEIPKSSKN"
     gene            1050..3167
                     /locus_tag="PKL17_07095"
     CDS             1050..3167
                     /locus_tag="PKL17_07095"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNK44530.1"
                     /translation="MKRHSTLFPVFLFGVVTACGDSGLPEPAGIQAELQLSNFGSCAD
                     LEQYVEDQAVFEMRTQLESMKRSGGSIDIFGRTTGVADSAAAPTAGAADSSKSSGPSA
                     YTKTNTQVAGVDEADFFKNDGTRIFQLVGQKLYAVKSWPANAMQIQSSMNIEGYPTQM
                     YLDEKNRVVVFSSIYTQYTGYLEDWAVGSGGGVAADVACGPGYCGGWYGNTTKVTVID
                     VSDLTNLKVQNEFFLPGNYANSRRVGSSVRLVLSDHFRWPSTVKFYPDSSTINYNDWR
                     AWNAELDRLKDANEKIIRDQTLDKWLPAQKVRTADGTVKTLPYNCSDFAKHNAPTHLG
                     ITTVATINLDAPQAAPQRSSILGEVGEIYANQNALYLTNRHWWWWPRFNQRDWTYLFK
                     FDLSDVNKARFVAAGGVDGHIVDQFSMDEDKGFFRVATTIAERVQDPKDPRNYWGIIQ
                     TTNRVTVMGQNGGKIQTVGQSEELAKGERIYSSRFMGDRGFVVTYRQVDPLYTFDLSN
                     PASPRKVGELKIPGFSTYIHPLDANNLLTIGIYTPETGTGPRSLQLSIFDVSDFANPK
                     QKWTQLVGSSYGWSEATYEHKAFNYFPERKMLAIPFSDWSYSGTDPWGSFRSELKVFD
                     IDVATGFKLRGSVSMKDVYQSAGLPTWAWYYSPWMRRSVMATDMGTDYVYAVSDAGIR
                     AVTGPTLGTTLSTVVFPRYDVLR"
     gene            3321..3674
                     /locus_tag="PKL17_07100"
     CDS             3321..3674
                     /locus_tag="PKL17_07100"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44531.1"
                     /translation="MRSLNFRSFVSALQVPVLAIGMLFASGCFKTTAIIPTYPMAMSE
                     TRTMWTNGFFWGAVGDTVDTGAVCGGRPVMRVTTDRSFGNAVVSWVTLGIYTPSRLRV
                     TCGQAPVHAGYYQNY"
     gene            3776..5113
                     /locus_tag="PKL17_07105"
     CDS             3776..5113
                     /locus_tag="PKL17_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44532.1"
                     /translation="MRIAVLSTALLSFVGLFSAACSPKDDLNVEQKPEQLLTYHQDAK
                     PILERYCTGCHTEGGIANFALTSYAKAKQFAPLLAGSVESGDMPPWPPSDDSLPLRYP
                     RKMEPAHKQLLLDWLKGGAQEGDATAPARVVIQSPERPAPPRADLVLDMGVTYQPNKN
                     MHDDYRCFVLDPNPSGTGGMPQDSWVRAGVVKPGNAAIDHHVIIFAIPPDKAALVKKK
                     DADEAGPGYSCLGGPGTWSASFLLGWAPGGVAVRLPDNEGMPVKKGTIFVMQVHYNVH
                     NDDGKGDRTTAELEIVKTPPQYQVYQLPLADPDRLKIPANDPDAIQSIVAPVRLILQE
                     LKLPGNDLTIVSVTPHMHLLGTQISTLVGSQPLVSIPKWNFHWQQAYQLTEPFVAANN
                     QSLILECHYNNTADNQPVIDGKKRTPRDITWGEGTEDEMCLSFVGIRVPRAQP"
     gene            complement(5165..5521)
                     /locus_tag="PKL17_07110"
     CDS             complement(5165..5521)
                     /locus_tag="PKL17_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44533.1"
                     /translation="MSIIQDFIREAGWPIFPTLAFGIAALAVSIKMIEKPTEQLASLV
                     RNLSCLTLIFGVFGTIVGLQYSVSAIEKVEPAMRWIVLIGLRETLNNLDAALLVLFPT
                     MLCYTAGKYKLTESKP"
     gene            complement(5688..>7424)
                     /locus_tag="PKL17_07115"
     CDS             complement(5688..>7424)
                     /locus_tag="PKL17_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK44534.1"
                     /translation="AVPEAVPEEVWSGRLGERLAPGSELAASALLEPPAPSSVSPIPF
                     AGPAPRIAVGLRDDSSQPLSFEAVAELLDSATTREDIIAALLRGARSRGAFAALFTKH
                     HDFVQGRLSLGTDFGQTDLIEKLVIPTDKASPFATALARSEPLACQILETDVGADVLK
                     ALERPVGVSALLVPVIVRAKSVALLYVDDDGSLIEGSVSQELRDAAAQAGQAWARLQT
                     EDRPGPTDKTEKSLLPLTTAPMPTVAPGTSIPAPVADQPTTPMRNKKGKSGDSPKPAP
                     AAEPAKAEGGKKDADAKKEVDLSALVDKAGNGDDDAFAKLLAAGEAGARAVVSALPGP
                     LRGADRRSIGDPTPTSAASQGPLLSLVLRLGEAAISPILERIANPKTPAEIRGYLIMV
                     LAEAPASSALTALGDLLCDRSETVRTAALSALRSFPPSAPLRALNKWLIDQLSKGDDR
                     RRSHAIEALVELRETSIVPELISLLSDADPACVETAQRALVILCKQDFGESPRRWNAW
                     WSKHGSEPRLSWLLRGLSHADQQVRALAQDELTQLSGDVAGYRSEQPKRERELAAKRW
                     TEWWQRRGYAVE"
     assembly_gap    7425..7524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7525..9320)
                     /locus_tag="PKL17_07120"
     CDS             complement(<7525..9320)
                     /locus_tag="PKL17_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44535.1"
                     /translation="MSKRLSSLLVEDGRVTSQRITDAIERQALNGGCLDSSLLELKAI
                     DEDLLIQYLKRAAQLPALPHGISGSERPLPEVLEVFTKSVAESHHAVPTRLLPGGVLQ
                     VLVTVEVDVADLDALAHVVKQRIEPWIVFEFRYQEAFDLVYGGGVATRFSRLLKRSNQ
                     THESKGWPAPLFVDPIPRFGSLDSLRLCPQPKVVEPKAAQPAAAQVEAPTLPTEVPPV
                     PTEVPPVPTEVPPLPTEVPPVPTEVPPLPIKHMEAEAVPAAAVSEPAVPTEVRDTVEV
                     PAIRTEVVLADATPAAPTSQPNIAEPVVRDTVKVPAIAATASPSEPSMSMDDWLSADD
                     WLSAASAVESKRTPSDKVVVSRRTPQPAAQPQPAKVEPAPVAAVSEPAPPAAIEKPIP
                     AEKPATPVAAEAKPVEKVSAPVVTTPPAQPAAKPAAVTPPAQGGKKKKGKHGREVTLA
                     TNAPTLGQLSGSKPQPQPQAKPQPQAQAKPQPQAQPQAKPQPQAQPQAKPQPQAQPQA
                     KPQPQAQPQAKPQPVSAPAVKPTERPVEAKPAQKAPEAKPAEKLAAKPVDTKPADKTP
                     ETKPAEKPAPVSPDSKPVDKQPAPVSAEAKPV"
     gene            complement(9317..9841)
                     /locus_tag="PKL17_07125"
     CDS             complement(9317..9841)
                     /locus_tag="PKL17_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNK44536.1"
                     /translation="MALVATSAVEPSIESASTPKTSFRAAGFFRRSTAMVFDLLLVVP
                     LQVLAVFGLRFIQRQPVPRLAELSPDVLLASLSDGSVKSEAMLVAGLAVTLLYYWLFV
                     GLRGQTPGKWLTRVMVVDVYGDRPSLLRAMVRTLSYGVAVLPCSLGVLWIGFDREKRG
                     LHDWLAGTYVVQKP"
     gene            complement(9880..10905)
                     /locus_tag="PKL17_07130"
     CDS             complement(9880..10905)
                     /locus_tag="PKL17_07130"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44537.1"
                     /translation="MARFRLRMRNMEINLPMGEIIIGRGPECYLRVDEPMVSRRHARL
                     RVSQTSITLEDLGSRNGSRVNGINATAVVQLNPGDILGIGTQQFTLDREDDPSPHRPT
                     VTHMPDWTQKSGNTNVSVSQSFSDVNVSPVSVGPQPAQAPAPPVPPSPSAPPIAIPSP
                     GSTISNPAPASSSISLPGGVPDHPATPITDGQTHVAPDSDSIETTGGANRGSAYRLFW
                     GLSDKVLAMGRIEDAERMMGPRLAEMRSRAEGGDIPEDVVIVEALRRALRLAGATKKD
                     QWFAWVFDYARVCRYKMPTALLDEIYAQVFACRPAVSARIVAYATTIDDDTIAVRLAT
                     LRRLCRE"
     gene            11067..13721
                     /locus_tag="PKL17_07135"
     CDS             11067..13721
                     /locus_tag="PKL17_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNK44538.1"
                     /translation="MQEPHDQPVPGTTQTPVDAAQQPTPTWNVQLDRAAVIAAELNLR
                     PQQVERTLALLDEGATLPFIARYRKEATEGLGEVEISTIAERGTYLTELQTRKAAVLT
                     EIHKQGKLTDLLKQRIVKTLSRTELEDLYLPFKPKRRTRATIAREKGLEPLAQLIWSQ
                     PDTAPSLDELATPYVNAEKGLLDVASVLSGARDIVAEQISDDAGVRSALRTFSLESGV
                     LKSRLIKRKDDSEEAPEPGNERAAGDVKPVVKTDKKDDPALKFSDYFEYEESAKTIPS
                     HRMLALRRGEKEGVLRVTLEVDGDRSRGVISQQVIRAPSSPLLGELKLAIDDAWERLL
                     KPAVEVDVRLVLRERADAEAIRVFAENLRHLLLQAPLGGKRVLALDPGYRTGCKLAVV
                     DEKGDLLAHDVIFATMSESRRIEAAERLEDLVREHKVSSIAIGNGTASRETEGFVRKL
                     AAAGKLPGIKIIVVSEAGASVYSTSEIGRQEFPTHDPTVRSAASIGRRLQDPLAELVK
                     IEPKSIGVGQYQHDVHQPTLKKQLDQVVESCVNQVGVDVNTASPKLLQYVAGVGETLA
                     QNIANYRSQHGPFSSRKQLLDVPRMGPKAFEQSAGFLRIRENAESPLDSSAVHPESYD
                     VVERMAKDLSVSVHELVGNSDLIRQIQIVRYVDDRRGEPTLRDILAELEKPGRDPRQE
                     FEEVGFNPDVTEVSHLKEGMVLNGVVTNVTNFGAFVDVGVHQDGLVHISELSHKFVKD
                     PAEAVKVGDRVKVRVIKVELDRMRIGLSIKAASEPPKPEPGDARRPPRAVDGGQPRGR
                     DGDRRREDGPRRDEGPRREEAPRRDDNRARPPAAQSGNARPPADNRPPRRDDAGPRPP
                     SRGDKPAAGGPKTSPFNSIRGLNLVLKK"
     gene            complement(13746..15677)
                     /locus_tag="PKL17_07140"
     CDS             complement(13746..15677)
                     /locus_tag="PKL17_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44539.1"
                     /translation="MHTITAAALCGTMLFSAPFAVADIPPNAIPQAAPKAQPNPTSGQ
                     QAPMGKAQIVATVRIANLSQSIKTLAGYVPFPLPVQESLQQVVGDFAKVINQAAPLDF
                     ALALEQDAATAMRGAPPWAVALSTTSAEETRKIAKSQGLLGENRNGQTQLRFPLGKND
                     VLYCVLQGQAGPGKLTCAPSERERDMLSPQLANLNVPGAQGKDLYAEVSVASLLGIFG
                     TQWKQVLDAGTMMLPQRLAIGDPKFDRAATDAVQALIGQVAMTGQDLSLLALDLTLRP
                     DQVQVALGYKMSGTQSAWAKADAAAAARKPIAPPAAFSALPKEVVSASYYVTDPRWSK
                     QVVELLLPLVDGFLAHDGLAEADRQAIRDLLLKQPKWDGVMTTVLGEGIGDKPAAAGD
                     PLTNMLSNHYYLSTAERENGGVDESPAFLRALAATWNRPGLQAYLKKKWKTVGIKSPI
                     PIIRAETVGKPFGPDAAGLYLSLDLSGWSSELDKATKGQKRGPFRVYLFSAQVAGRVW
                     SAVAADKNLALQKLQRLANLPASETLASRSGLAQVQQPGWQSAGFTTLAGWIGLFDTV
                     MRVAEKSPLGKGAAPGQTGTSLLNVIPHHGEVPITYGAQGGSIGPQGMTKVMSASIPR
                     LVIEDLIALAMNIGGGGKK"
     gene            15824..17326
                     /locus_tag="PKL17_07145"
     CDS             15824..17326
                     /locus_tag="PKL17_07145"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNK44540.1"
                     /translation="MKFSFRTVDSPAMVLAEKADQLAIPLGEGSLQSDALLLAIDAQL
                     GGTLRHTIDDEQFLGKASQSLNLHSLGRLGATRLLLLGTGKPGAVLLADLRTWTARAV
                     RIAGAKVKRLALCLSGVAVEQAALVGQAVAEGAILASYQFDKYVTGDRKRSFLLEEVV
                     VFLGAGASIEAAEHGRRRGEIISGGVILARDLANEQPTVMCPRKLAEVAADVAERYGL
                     SCTIRGVKECEQLGMGMFLAVGRGSTEEARFIHLTYRPSTPPRRKVALIGKSVTFDSG
                     GLSIKTTEGMLWMKMDMGGGAAVLGAIQAIAQLGSADEVHVLLAAAENMPSGNSYKLG
                     DVLRSMSGKTVEINNTDAEGRLTLGDAMTYAVEKIGVDEIIDLATLTGSSMVALGNFT
                     GALFSNSDTLAGRLLEASQQGGDDLWRMPLRDRLFDSLKSDIADMKNSGDRYGGSISA
                     ALFLREFIGDKTWAHIDMAGPIHADKDFGHVSKGATGFGVATLVELLSPR"
     gene            complement(17337..17408)
                     /locus_tag="PKL17_07150"
     tRNA            complement(17337..17408)
                     /locus_tag="PKL17_07150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17374..17376),aa:Thr,seq:cgt)
     gene            complement(17465..18406)
                     /locus_tag="PKL17_07155"
     CDS             complement(17465..18406)
                     /locus_tag="PKL17_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNK44541.1"
                     /translation="MWGPTFALLLLATPGTADGVALSAVSGLLPSGALPYSVLSALSS
                     DRLRALTPDGEGQSKQPHSVFTKLSQSGKEKGCCGYPLGETGTYKLTYYWLAYESEYA
                     NEPYEIEIYTRQGFPLGRFPRAFVFELRLEGSGVLRDGRLINYDGRCHYGIGTCYQTL
                     DPQAHPFGKGGQQRALQPFRSVAVDPRWVPLGTPLYMPELKGMRLPDGSYHDGCVRAD
                     DTGGGIRRREIDFFVESYANYKYLEEQLWNDHKVTPHVEEPRCQYLRQFDPGVDRRSE
                     SIDETATAFVRRPPPKPPSAATTKKGSQRRTTRKKQA"
     gene            complement(18456..21437)
                     /locus_tag="PKL17_07160"
     CDS             complement(18456..21437)
                     /locus_tag="PKL17_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK44542.1"
                     /translation="MSAALPKAWLLTISAGYLLIGLLACVAAFRRGRLALLLPTLIAA
                     LLSGMLAAVGYGLFALHLWEPAFLSSGRELLGESLRSAVDGGLAIRIGPRVVVVEQPL
                     WLLLVSAIPWLIPGLLVSLTDMSRKQLWLQLVLRSSLLIVLSTALARPALLGDRQKLS
                     VVALVDVSDSVSSSQLARVSAQIETLRERTRSRGDDLHIVRFGGQPRLFPQPAPGVPL
                     PPLRAPTTRSDDADPTASNLQAALQLSYGLHAPGTLRRTILFSDGNQTEGDVLGEAVA
                     AARRRVHVSVFPLKASDSPEVLVRELRLLGGPGADAQLKVGAPFTLEAEVYSSVQQTA
                     RLELLQNGSPNPDDAHRDIELTPGRNLVRFRSEPKGPGALTYQARLVAKPNSGTQAAT
                     PLSDTIATNNQAQLSLLAKGKPRVLYIEGESTAKGYLTQALQRENIDVEARGPYGMPS
                     SAKDLLPYDLVILSDVSATLVSQAQMLAIESYVRDLGGGFVMVGGENSFGSGGYQGTQ
                     LEKLLPVRFEQEKKREQPSLAMVLCIDRSASMNGPKLELAKDAARATAEMLAPDDLIG
                     VVAFDSQAIPLVRLQRAQNRIRISTDIARLTAGGGTQLLPPLNEAYQQLVSANAKIKH
                     VILLTDGQAEYQGIIELTDQMVQNKITVSTVGVGAGADQTLLTAIAEHGGGRFYYTQD
                     ASSVPKIFTKETTQVARSALIEERVVPKVAKFVELLDGVSIDNAPPLRGYVSTKAKPL
                     AEVILVSPLGEPLLARWRQGLGQAVAFTSDAKNRWAVDWLRWPGYAKFWAQLVRSSMR
                     HDGQRSGHGYDLQTMVAPPLLRVRVDAVSADDRFLSGLQTELEVIAPAEGGIGLIAGK
                     GDIVERKEMSLVAPGRYEAEVRLGRTGSFLLRAIHRSPSPSPSTPGPIVAESWGTLSL
                     PYPREYLALPPDDLLLAQVATLGHGKLLQDATSVLDPGDEKIRFLRALWPSLLWVAIG
                     LLLIDVLLRRLRIFGFRPLSL"
     gene            complement(21434..23422)
                     /locus_tag="PKL17_07165"
     CDS             complement(21434..23422)
                     /locus_tag="PKL17_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK44543.1"
                     /translation="MTPLSLLGLPWNQLLPIAAAGSAVLTVLYVLRQRRRRLEVPFSP
                     LWQKVLGQSEAQSLWQKLLRVLSLLLQLVILGLLTLSLGDPRLGRSTEGRSLVLLIDA
                     SASMQAQVPLLPGKTRLDLARAEAEQIIRGLHGDDLAVVVGLDGRPAPLGGFSDDERE
                     LLAQLHQVEARDTGADLQAGLLLAEALLRGRPHPQVVLFSDGGFDEAALRKKPAALDV
                     RFVPLPSQARLRPGDGNAAITSFAVRRYRRNRLSYEVLLQLSYFPSPQSPPGAKTATL
                     ELRQEGELVDVQKLDLVPGEQTRKLYPSLSGAGTHLQATLKLEGGEVDLLPLDNHAYA
                     VLPERRRVKMLIVTRGNLFLEGALLAASAGEENHLAIDKITPSDYSDDKAARYDAVLF
                     DAFTPPRPPDAHAIYLDPQGADSPFAIAKTVNAPYLSDVDEQHPVLRWVSLADLNMSR
                     ASVFRLGPFDRPLAKMIKDPIVIAREQPGPLGMRRAVALGFDVRQSDLPLRVAFPVLL
                     MNAIDWFAGDVDEDLGSFRTGTTLFLPLRDNPSKTRGPDTKLRELQKADLTQPGDVHT
                     MVPVHEGIVKLYGEKAGFYSLRVPELLRGIEFALNLADGAESAALLRTELQLERTTLV
                     RPDAGQVALRRTLWPYLLLVALLLLLCEWWTYHRRWTV"
     gene            complement(23419..24369)
                     /locus_tag="PKL17_07170"
     CDS             complement(23419..24369)
                     /locus_tag="PKL17_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNK44544.1"
                     /translation="MEKAAASTQAAQAGFDQQFLKQLEYLHVVAKKVFAGKSRAERRS
                     RGQGSGIEFADHRDYTAGDDLRYLDWSVYGRMDRLLLRLFEQEEDLHIYLLIDRSASM
                     RLGGKPDVPLLRRGTDGRMAKLGYAAQITAALAYVGLANLDRVAIYGLGERLSAELPA
                     SRGKGQIFKVFDFLANLKPDGQTDLKASIGEFVQRIKRRGIAIVLSDFYDHHGYEEGL
                     NLLRYHRFEPAAIQIIDPIESNPPLRGDLEIVDMETGELREVTLSQSLIEAYQREHQS
                     YCDALAAFCKSRSVSYIRAETGVPFDELTLRALRQGGFIR"
     gene            complement(24377..25447)
                     /locus_tag="PKL17_07175"
     CDS             complement(24377..25447)
                     /locus_tag="PKL17_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK44545.1"
                     /translation="MSQVAEPATAEQAAQQIQRFRKDVRRLRDEIARVIVGHEEIVEG
                     VVIALLSGGHVLLEGVPGLGKTMLVRTLAEAIDLSFSRVQFTPDLMPADILGTNMLVE
                     EESGRRRFEFQRGPIFAHIVLADEINRATPKTQSALLETMQEQTVTVAKTTYQLPQPF
                     FVLATQNPLEMEGTYPLPEAQLDRFFFKLRVEFPSSEALHTILDRTTSQTSVHATKVL
                     GGPELLELRSLVRQVPVARTVQDYAVRILIGSHPDAEQAQKALGKVEKTDRNDLVRRF
                     VRYGASPRGAQALLLGAKVLALLDGRFAVSCADVAKVARPALRHRLILNFEGQAEGVS
                     TDDIITAIISRTPEAIPGTARS"
     gene            complement(25594..25986)
                     /locus_tag="PKL17_07180"
     CDS             complement(25594..25986)
                     /locus_tag="PKL17_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNK44546.1"
                     /translation="MARPTLLSLKAQLQKLRDEVVHQGPAKIEPNRKDATTIGVSDED
                     EQALSEMMQTLASQQNRRQSELLGQIDRALLRLDREPDMVGLCEECEEEIDERRLSLR
                     PYVTLCADCQADHDPKRNQSRKSLTDYR"
     gene            complement(25989..27233)
                     /locus_tag="PKL17_07185"
     CDS             complement(25989..27233)
                     /locus_tag="PKL17_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNK44547.1"
                     /translation="MSQTKKSPTNTAASSEFAAMLAEFEKEQKSSPARRKQEGPKVGA
                     EVRGRILSIGRDSAFVDLGGKDDGAIPLDELRGPDGQLTAQVGDEITARIVEIDGRGG
                     GVVLRRTLGRGPDARGELRQAFELGIPFEGTVSGVVKGGVEVQVAGVRGFCPISQLDL
                     RHTEDASGYVGQKLQFRITRFEENGRSVNVVLSRRALLEEAAQALAEELRKKLAPGVV
                     ARGRVTALKDYGAFIDLGGIEGMLHVSELSFQRARLPKDVLSVGQELEVAILKIEPSQ
                     DPKRPERISLSLKSLEKDPWSDIETQFPVGTRFPATVLRTEAFGAIVELVPGIEGLVH
                     ISELGGGKRLRHAKEAVQPGAKIEVVIQQIDRDKRRLSLGMASPTDVEGGEAAPSAPS
                     GGGRVGFGTLGDLLSKASQKKK"
     gene            complement(27303..28682)
                     /locus_tag="PKL17_07190"
     CDS             complement(27303..28682)
                     /locus_tag="PKL17_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNK44548.1"
                     /translation="MAQTVFHKLWQRTTAVTMCAAVLIALSVPRTALAKACPNLVILI
                     DQSASMAQNSLGQSVPQGSPDSKWSIATKALTNMNNKYDGLLPLGYCNFPNPTGCSVN
                     AKIPIPPGYSNRIAINNAMITYPFSGGNTPTCTAVTSAAADLVKSDPTRPGYLLLVTD
                     GSPDALCCGADPVKTTVDAIRAAANPTVAGTPPVYTMVLGFGKTLDSERASLNLMADA
                     GGFPLTQDPTYHYYRAEDATALEQALATIVKSVVGGDAGTIATCEDGCYAKGCPGGQV
                     CYQNSCRANPCATKTCPSSQYCLPTFYANGTSNAECVDACRSPCPQTSRCDRGKCVGD
                     PCSGQCLPGQKCQPIPDSSFGTCVDEPLCKNTICHQGQGCFAGATDGQCVDNLCNYVT
                     CPQGTVCHPFTGACESPDPQPVLSLGAGGIGCDVSQTASSASRLSWLSSGLLLSLLLL
                     SRRRGAGRA"
BASE COUNT         5390 a         8548 c         9193 g         5537 t
ORIGIN      
        1 tggcaaactg cgcggttcag ggggcatcgg ccccgccaca gattgtgcaa gcgatcgaaa
       61 ggctggcacc gcacgtcgac gtgatgatcg tggggcgggg cgggggatcg gtggccgatc
      121 tgtggtgttt taacgatgag caagtcgccc gcgcgatcca tcgctgcccg gtgccggtgg
      181 tgtcggcggt gggtcacgag gtggacttca ccatcgccga ctttgttgcg gacgtgcggg
      241 ctccgacgcc gtcagcggct gccgagctat gtgttccgga acatgcgcgg ctttttcgcg
      301 aactgggtga actgagccgt cgcctgctgc gggcggggcg gcggcttgtg gacggtggca
      361 gacaacagat cgatcagctg cttgggcgtg ggcagatggc gatcgagcgg aacttgatgc
      421 aaaagcgtcg cacgctcggg gatctgggca agcgacttac tgcttgtcat cccaagacgc
      481 agcttctgcg ggaccaggga cagatcgaca agctgcacag tcgactggtt gctgcgatgg
      541 agaagcagac gagcgagcgc cgtcagaacc ttctgtcgct gactggatcg gacgacgaac
      601 gcctggtgcg ggccgtgcag cggttgcttg ccaccaaacg gcagcagctt gtgggcgaag
      661 tcggaaagct ggaagcgctg tcccccctca aagttttgac ccgtggtttt gccgtggcgc
      721 gcgatgcgga aggccgtgtg cttcgtgatt ctgcggcagt gaccgacgga gattccatct
      781 cggttcagct tgcgcaaggg gcgctgtttt gcacggtgga ttcgcgttcg ctaccgcgct
      841 ctcagaattt cgagatcccc aagtcatcaa aaaactgagc ttgcggcgtt tcccatgtcc
      901 gttcgaaacg gacagtttcc gtcgctgctg tcggcgttcg ttcgatctgg catggccttc
      961 gcaagagtcc atgggcaaga agccaccttg catggggctg cggcgtttcg tagccctttt
     1021 tcttaaggct ttccgtagga gaaatcgtca tgaagcggca ttcgactctg ttccccgtgt
     1081 tcctttttgg tgtggtgacg gcttgcggcg acagcggtct tcccgagccg gcagggattc
     1141 aggctgagct ccagctctcg aacttcggta gctgcgcgga tctcgagcag tacgtcgaag
     1201 accaagcggt tttcgagatg cggacgcagc ttgagtcgat gaagcgcagt ggcggcagca
     1261 tcgacatttt cggtcgaacc accggcgttg cggattcggc tgcggcaccg acagcaggcg
     1321 cggcggactc gagcaagtct tctggcccaa gtgcgtacac caagaccaac acccaggttg
     1381 ccggtgtcga cgaagcagac tttttcaaga acgatggcac ccgcattttc cagctcgtcg
     1441 gacagaagct ctacgcagtc aagagctggc cggctaacgc gatgcagatt cagtcctcga
     1501 tgaacatcga gggctacccg acccagatgt atctcgatga gaaaaaccgc gtggtggtct
     1561 tttcatcgat ctacacccag tacacgggct acctggaaga ctgggcggtc ggcagtgggg
     1621 gcggtgtggc ggcagatgtc gcctgcggtc ctggctactg cggcggttgg tacggcaaca
     1681 ccaccaaagt cacggtcatt gacgtttctg acctgaccaa cctgaaggtg cagaacgaat
     1741 ttttcttgcc gggcaactac gcgaactcgc gccgggtggg atcgtcggta cggctggtgc
     1801 tgtcggatca cttccgctgg ccatcgaccg tgaagtttta tccggactcc tcgaccatca
     1861 actacaacga ctggcgcgcg tggaacgccg agctggatcg gctcaaggac gcaaacgaaa
     1921 agatcatccg cgaccagacg ctggacaagt ggctgccggc ccagaaggtg cggaccgcgg
     1981 acggtacggt gaaaacgctg ccgtacaact gctcggactt tgccaagcac aatgcgccga
     2041 cgcatctggg tatcacgacg gtggcgacca tcaacctgga tgcgccgcaa gctgcaccac
     2101 agcggtcgtc gatcctgggc gaagtcggcg aaatctatgc caaccagaac gcgctgtacc
     2161 ttaccaaccg tcactggtgg tggtggccgc gcttcaatca gcgcgactgg acgtatctgt
     2221 tcaagtttga tctgtcggat gtgaacaagg cgcgtttcgt cgcggctggt ggtgtcgatg
     2281 gccacatcgt cgatcagttc tcgatggatg aagacaaggg gttcttccgc gtcgcgacaa
     2341 ccatcgccga gcgcgtccaa gatcccaaag acccgcgcaa ctactggggc atcatccaga
     2401 cgaccaaccg cgtcactgtg atgggtcaaa acggcggcaa gatccagacc gtgggacaga
     2461 gtgaagagct ggccaagggc gagcggattt actcctcgcg attcatgggc gaccgcggtt
     2521 ttgtcgtcac gtaccgccag gtcgatccgc tctacacgtt cgacctgtcg aatccggcga
     2581 gcccgcgcaa agtcggcgag ctgaagatcc ccggcttttc gacgtacatt catccgcttg
     2641 atgcgaacaa cctgctgacc atcggcattt acacgccgga aaccggcacc ggaccgcgct
     2701 cgcttcagct cagcattttc gatgtgtcgg acttcgccaa tccgaagcag aagtggaccc
     2761 agcttgtggg ttcgtcttac ggctggtcgg aagcgacgta tgaacacaaa gcgttcaact
     2821 acttcccaga gcgcaagatg ctggcgattc cgttttcgga ctggtcctac tcgggcaccg
     2881 atccatgggg atcgttccgc agcgagctga aggtgttcga cattgatgtc gcaacgggct
     2941 ttaagctgcg cggatcggta tcgatgaaag atgtgtacca gagcgccggc cttccgacct
     3001 gggcgtggta ctacagcccg tggatgcggc gcagcgtgat ggcgacggac atgggcaccg
     3061 actacgtcta cgcggtcagc gatgccggca tccgcgccgt gacgggtccg acgctcggta
     3121 cgacgctgtc gacggtggtt ttcccgcgct acgacgtcct gcgatagttc ccctcctgtt
     3181 tgcggcgcag gctggttgtg tctgcgctgc ccccgcaaaa aaattttcag atgcggtgac
     3241 acatcttggc ggctgcggtg tttgagatgg caagcagaga tgctgcaaat ccaaacccca
     3301 cacgtcgctg aggtgatgtc atgcgtagcc taaactttcg ttcgtttgtt tctgcccttc
     3361 aggttcccgt gcttgctatc ggaatgttgt ttgcgtcggg ctgcttcaag acgaccgcaa
     3421 tcattccgac ctatccgatg gcaatgtcag aaacccggac catgtggacg aacggatttt
     3481 tctggggagc cgtcggtgac accgtggaca ccggggctgt ctgtggtggt cgtccggtga
     3541 tgcgcgtcac cacggaccgc agctttggca atgcggtggt gtcttgggtg acgctcggaa
     3601 tttacacgcc gtcgcgactg cgcgtgacct gcggccaggc gcccgtccat gcgggctatt
     3661 atcagaacta ctgatcgcaa catgtctttg gacttcgcga agactggttc ccattccttc
     3721 gcagactctg ggctatgatt cgcgccgctt gtcttttccc ttgcggaggc tttcgatgcg
     3781 aatcgctgtc ctgtccactg ccctcttgtc tttcgtcgga cttttttcgg cggcatgttc
     3841 ccccaaagac gatctcaacg ttgagcagaa gcccgaacaa ctgctgacgt accatcagga
     3901 tgcgaagccg attctggaac gctactgcac gggatgtcat acagaaggcg ggatcgcaaa
     3961 ctttgcgctg accagttacg ccaaagccaa gcagtttgcg ccgctcttgg ccggttcggt
     4021 ggagtccggc gacatgccgc cgtggccacc gagcgacgat tcgttgcctt tgcgctatcc
     4081 gcgcaagatg gaacctgcgc acaaacagct gctgctcgat tggctcaaag gcggtgcgca
     4141 ggaaggtgat gcgactgcac ctgcgcgggt cgtcattcaa tctccggagc gaccggcgcc
     4201 gccgcgtgcg gacctggtgc tcgacatggg cgtgacgtat cagcccaaca agaacatgca
     4261 cgatgactat cgctgcttcg tgctggaccc gaacccgagt ggcacgggcg gaatgccgca
     4321 ggattcgtgg gtccgcgctg gtgtggtcaa gccgggcaac gcagcgattg atcaccacgt
     4381 catcatcttt gcgattcctc ctgacaaagc ggcccttgtc aaaaagaagg atgcggacga
     4441 agcggggcct gggtatagct gcctgggtgg tcccggaact tggtcggcgt cgtttctgct
     4501 cggttgggca cccggtggcg tcgcagttcg gcttccggac aacgaaggaa tgcccgtcaa
     4561 aaaaggcacg atcttcgtaa tgcaggtcca ctacaacgtg cacaacgatg acggcaaagg
     4621 ggaccgcacc accgctgagc tagagattgt aaagacgccg ccacagtacc aggtgtacca
     4681 gctaccgctg gcggaccccg accgactgaa gattccagcc aacgatccgg acgccatcca
     4741 gagcattgtc gcgcccgtgc ggctgatcct tcaggaactg aagctgcccg gcaatgatct
     4801 gacgattgtg tcggtcacgc cgcacatgca ccttttggga acacagattt cgacgctggt
     4861 gggaagtcag ccgctggtgt cgattccgaa atggaacttc cactggcagc aggcctatca
     4921 actgaccgag ccgtttgtgg cggccaacaa tcagtcgctg attctggagt gtcactacaa
     4981 caacactgcc gacaaccagc cggtgataga tgggaagaaa aggacgccgc gcgatatcac
     5041 ctggggagaa ggcaccgaag atgagatgtg cctaagcttc gtgggaatcc gcgttccgcg
     5101 ggcgcagccg tagttgccgg aatccgtcgc cgtgccttag gggaaacggc gaatcatcct
     5161 tccgctatgg cttggattcg gtcagcttgt actttcccgc ggtgtaacag agcatcgtcg
     5221 ggaacaggac cagcagcgcc gcgtccaggt tgtttagcgt ctcgcgcaga ccaatcaaga
     5281 caatccagcg catcgccggc tcgacttttt caatcgcgct gaccgagtac tgaaggccga
     5341 cgatggttcc gaacacgccg aagatgagcg tcagacagct caaattgcgg accagcgagg
     5401 caagctgttc ggtcggcttt tcgatcatct tgatcgagac ggcgagtgcg gcgattccaa
     5461 atgccagggt cgggaaaatc ggccagccag cttcgcgaat gaaatcttga atgatcgaca
     5521 tgggacacct ccttgggatg ttgctcttgg gttcagtctg accctattgc agccgctgtg
     5581 ccaagccgcg gcacgtcgtg ttgcgaggag aactgagttg tcgcaaggag gaatgcgtca
     5641 caaagggagt gtccgcggac gtccgcaggt gtccgggggg acgagggcta ttcgaccgcg
     5701 tagccgcgcc gctgccacca ctcggtccag cgcttggcgg ccagctcgcg ctcgcgcttt
     5761 ggctgttccg atcggtagcc ggcgacgtca cccgaaagct gggtcagctc gtcttgggca
     5821 agcgcgcgaa cctgctgatc cgcatgcgac agaccgcgca gaagccagct cagccgcggt
     5881 tcggacccgt gcttggacca ccaggcgttc cagcgccgcg ggctttcgcc aaagtcttgc
     5941 ttgcacagga tcaccagcgc ccgctgagcc gtttcgacgc aggcggggtc ggcatcggac
     6001 agaagcgaga tcagctccgg cacaatcgag gtttcgcgta gctcgaccag cgcttcgatg
     6061 gcatggctgc ggcgacggtc atcgcccttg gacagctggt cgatgagcca cttgttgagt
     6121 gcgcgcaggg gggcactcgg cggaaacgat cgcagggcgg aaagcgccgc agtgcggacg
     6181 gtctcgctgc gatcgcacag cagatcgccg agcgcggtca gggcggacga tgcgggggct
     6241 tcagccagca ccatgatcaa gtagccacgg atttccgctg gggtcttggg attggcgatg
     6301 cgttcgagaa ttggcgaaat ggccgcttct ccgagccgaa gaaccagcga caaaagcggt
     6361 ccttggctgg cggcagacgt cggtgtcgga tcgccaatgc tgcggcgatc ggctccacgc
     6421 agcgggccgg gcagggccga taccaccgcg cgcgcaccgg cttctccagc agcgagcagc
     6481 ttggcaaacg catcgtcatc accatttccg gccttgtcga caagcgcgga aagatcgacc
     6541 tcttttttgg cgtccgcgtc ctttttgcca ccctccgctt tggcgggctc ggctgccggc
     6601 gcgggctttg gtgagtcgcc gctcttgccc tttttgttcc gcatcggcgt cgtcggctgg
     6661 tcggcaacgg gcgcagggat gctagtaccc ggagccaccg ttggcatcgg tgcagtggtc
     6721 agcggcaaga gcgacttttc ggtcttgtcg gtcggaccgg gccgatcttc ggtctgcagt
     6781 cgcgcccatg cctgacccgc ttgcgcggcg gcatcacgca gctcctgact gaccgatcct
     6841 tcgatcaggc tgccatcgtc atcgacatac agcagcgcga ccgacttggc ccgcacgatg
     6901 accggaacca gcaaggccga cacgccgacc ggacgctcca gtgcctttag cacatcggcg
     6961 ccgacgtcgg tttcaagaat ctggcacgcc agcggttcac tgcgcgcaag tgcggtcgca
     7021 aacggcgacg ccttgtccgt cgggatcacc agcttttcga tcagatcggt ctgcccaaag
     7081 tcagtaccga gcgacagccg gccctgcaca aagtcatggt gtttggtaaa cagggcggca
     7141 aacgccccgc gcgatcgagc accacggagg agtgcggcga tgatgtcttc gcgtgtcgtc
     7201 gccgaatcga gcagctcggc gactgcttca aacgaaagcg gctggctgct gtcgtcgcga
     7261 agtcccacgg caatgcgtgg cgccggaccg gcaaacggga tgggcgatac agacgacgga
     7321 gcgggcggtt cgagcagcgc gctggccgcc agttcgctgc ccggagccag gcgctcgccc
     7381 aagcgaccac tccagacctc ttcgggcact gcttcgggga ccgcnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnntcgaca ggctttgcct cggcagaaac cggcgcgggt
     7561 tgtttgtcga caggctttga atctggcgaa accggcgcgg gcttttctgc cggcttggtc
     7621 tcgggagttt tgtccgccgg tttggtatcg acgggcttgg ccgccagctt ttccgcaggt
     7681 ttggcttctg gggccttttg tgcaggcttt gcctcgaccg gtctttcggt tggcttcacc
     7741 gcaggagcgg agactggctg cggcttggct tgcggctggg cttgaggctg tggtttggcc
     7801 tgcggctggg cttgaggctg tggtttggcc tgcggctggg cttgaggctg cggcttggcc
     7861 tgcggctggg cttgaggctg cggcttggcc tgggcttgag gctgcggctt ggcttgcggc
     7921 tgtggctgtg gttttgatcc cgacagctgg ccgagcgttg gcgcattggt cgccagcgtg
     7981 acttcgcgtc cgtgcttgcc cttcttcttt tttccaccct gagcaggcgg ggtcactgcg
     8041 gctggctttg cggctggctg ggccggtggg gtcgtgacga ccggcgcgct gactttttcg
     8101 acaggctttg cctcggcagc gacaggtgtt gccggttttt ctgccgggat cggcttttcg
     8161 atcgctgcgg gcggagcggg ctcgctgacc gcggcgacgg gcgctggctc gactttggcg
     8221 ggctgtggct gggcagccgg ctggggcgtg cgtcgcgaaa ccacaacctt gtcgctgggc
     8281 gtgcgcttgc tttcgactgc acttgccgca gacagccagt cgtctgcgga cagccaatcg
     8341 tccatcgaca tgctgggctc gctcggcgat gccgtcgcag cgatcgctgg aactttgacc
     8401 gtgtcgcgca cgaccggctc ggcaatgttc ggctggctgg tcggggcggc tggggtcgcg
     8461 tcggcgagca caacctcggt gcggatggcc ggcacttcga cggtgtcgcg aacttcggtg
     8521 gggaccgcgg gttcgctgac agctgctgcc ggcactgcct cggcttccat gtgcttgatg
     8581 ggcagcggtg gaacctcggt tgggaccggc ggaacttcag tcggcagggg cggaacttca
     8641 gtcggaaccg gcggaacttc agttgggacc ggcggaactt cggttggcag cgtcggcgct
     8701 tccacttggg cggcggcggg ctgggcggct ttcggctcga caacttttgg ctgcgggcag
     8761 agccggagcg aatcgaggct gccaaaacgc ggaatcggat cgacgaaaag cggtgccggc
     8821 cagcctttgc tttcgtgggt ctggttgctg cgcttgagca gtcgggaaaa gcgagttgca
     8881 acaccgccgc cgtacaccaa atcaaacgct tcttggtagc ggaactcgaa gacaatccac
     8941 ggctcgatgc gctgcttgac gacgtgcgcg agtgcgtcca ggtccgcgac atcgacttcg
     9001 acggtgacca gcacctgcag cacaccgcct ggcagaagcc gggtcgggac cgcgtgatgg
     9061 gactcggcaa cggacttggt gaagacctca agcacttctg gaagcgggcg ctcgctcccg
     9121 gaaatgccat gcggcaaagc cggtagttgc gcggcgcgct tgaggtactg gatcagcaga
     9181 tcctcatcaa tggctttgag ttccagcagg ctgctatcga gacagccgcc atttaaggcc
     9241 tggcgttcga tggcgtccgt gatgcgctgt gacgtgactc gcccatcttc gacgagcagt
     9301 gaagagagtc gtttgctcat ggcttctgca ccacgtaggt tcccgccagc cagtcgtgga
     9361 ggccgcgttt ctctcgatca aaaccaatcc acagtacgcc gagcgagcac ggaagcaccg
     9421 ccacgccgta gctgagtgtc cgcaccatcg cgcgcaaaag ggatgggcga tctccgtaga
     9481 catcgacgac catcactcgg gtcaaccact tgcccggcgt ctgtccgcgc agtccgacaa
     9541 acagccagta gtacaagagc gtgaccgcga gtccggccac aagcatcgct tcgcttttga
     9601 cgctgccatc ggaaaggctg gccagcagca catcagggct tagctcggcg agccgtggca
     9661 ccggctgacg ctgaatgaag cggagcccga agaccgccag cacttgcagc ggcacgacca
     9721 gcagcagatc aaacaccatc gccgtagaac gacggaagaa gcctgcggcc cgaaacgatg
     9781 tcttgggggt tgatgccgac tctatggagg gctcaaccgc agatgtggcg actaatgcca
     9841 tgggattact atattttccg gcaaggcgaa ttacaatcgt tactcacgac acaggcgacg
     9901 taacgtagcc aggcggacag cgatcgtgtc gtcgtcgatt gtggtggcgt aagcgacgat
     9961 tcgtgcagaa acagcgggtc ggcaggcgaa cacctgggcg taaatttcat cgagcagagc
    10021 cgtcggcatt ttgtaccggc acaccctggc gtaatcgaag acccaggcaa accactgatc
    10081 ttttttggtg gcccctgcga gtcggagcgc ccggcgcagc gcttcgacga tcacgacatc
    10141 ttcggggatg tccccacctt cggcgcgtga gcgcatttcg gcgagccgcg ggcccatcat
    10201 gcgctcggca tcctcgatgc gacccatcgc cagcactttg tccgataggc cccagaacag
    10261 acggtaggcc gagccgcggt ttgcgccgcc cgtggtttcg atggaatcgc tatcgggcgc
    10321 aacgtgggtc tgaccgtccg tgatcggggt tgccggatga tctggaacac cacccggaag
    10381 agatatactg ctcgacgccg gagccggatt gctgatggtg ctgccggggc tcgggatggc
    10441 aatcggcggc gccgacggac tggggggcac gggcggggca ggggcctgcg cgggctgcgg
    10501 tccgaccgaa acgggggata cgtttacatc actgaagctc tgcgagacgc tgacattggt
    10561 gttgccggac ttttgtgtcc agtccggcat atgcgtcacg gtgggtcggt gcggcgatgg
    10621 gtcgtcttcg cgatccagcg taaactgctg ggtcccaatg cccaagatgt cgccgggatt
    10681 gagctgtacg acggcggtgg cgttgatgcc attgacacgc gagccgttgc gacttcccag
    10741 gtcttcgagg gtgatgctgg tctgactcac ccgcaagcga gcgtgtcgcc gagacaccat
    10801 gggctcatca acccgcagat agcactctgg gccacgacca atgatgatct cgcccattgg
    10861 caggttgatc tccatgttgc gcatgcgcag cctaaatcgg gccatggtcg tattaagtta
    10921 ccgccgattg ggcacttctc acaagaggcg cgtgctgtgg gtgggcttct tgggtcgcgg
    10981 acggggactc gctgggcaag ataacggggg gtcgcgccgg ttcggtcttg atctccgtcg
    11041 ggcatctagt gtacgagtga cccgttatgc aagagccgca tgaccagcct gttcctggta
    11101 ctacacagac acccgtcgac gctgcccagc agccgacccc cacatggaat gttcagctcg
    11161 atcgcgccgc cgtgattgca gcagagctga acctccgtcc gcagcaagtg gagcgaacgc
    11221 tcgcccttct agatgaaggg gcgacgctgc cgtttatcgc gcgctaccgc aaggaagcga
    11281 ccgagggact tggcgaagtc gaaatttcga cgatcgccga gcgcggcacg tatctcactg
    11341 agctacagac tcgcaaagcc gcggtgctga cggagataca caagcaaggg aagctaacgg
    11401 acctattaaa gcagcggatc gtaaagacgc tgtctcgcac ggagctagaa gacctgtatc
    11461 taccgtttaa gccgaagcgg cggacgcggg cgaccatcgc gcgggaaaaa gggctggagc
    11521 ctttggcgca gctcatctgg tcacagcccg atacggcacc gtcgctggac gagctggcca
    11581 cgccgtatgt gaacgcggaa aaaggtctgc tcgacgtcgc gagtgtgctt tcgggtgcgc
    11641 gcgacatcgt ggccgaacag atttcggacg acgcgggtgt gcgctcggcg ctgcggacgt
    11701 tttcgctgga aagtggcgtg ctcaagtcgc gcctgatcaa acggaaggac gattcggaag
    11761 aagccccgga gccaggcaac gagcgggccg caggcgacgt caagccagtg gtcaagaccg
    11821 acaaaaaaga cgatccggcg ctgaagtttt ccgactactt cgaatacgag gaaagcgcca
    11881 agacgattcc gtcgcaccgg atgctcgcgc tgcgccgtgg tgaaaaagaa ggcgtgctgc
    11941 gggtgacgct ggaagtcgat ggcgacagga gccgtggcgt catcagccag caggtgattc
    12001 gggccccaag cagtccactt ttgggtgagc taaagctcgc gatcgatgac gcctgggaac
    12061 ggctgctcaa gcccgcggtc gaggtcgatg tccgtttggt gctgcgtgag cgtgccgatg
    12121 ccgaggcgat ccgggtgttc gcggaaaacc ttcgccacct tttgcttcag gcgccgctcg
    12181 gtggcaagcg ggtgctggcg ctcgaccctg gttatcgcac cggctgcaag ctggccgtcg
    12241 tcgatgaaaa gggggatctg ctcgcgcacg acgtgatctt tgcgacgatg agcgagtccc
    12301 gacggattga agctgcggaa cggctcgaag accttgtgcg agagcacaaa gtcagctcga
    12361 tcgcgatcgg caacggcacc gcaagtcgag aaaccgaagg ctttgtccgc aagctggctg
    12421 ccgctggcaa gctgccgggg atcaagatca tcgtggtcag cgaggcgggt gcttcggtgt
    12481 attcgacatc ggagattggc cggcaagagt ttccgacgca cgatccgacg gtgcgcagtg
    12541 ccgcttcgat tggacgtcgt ctgcaagacc cgctcgctga gctggtgaag attgagccga
    12601 agtcgattgg cgttggtcag taccagcacg acgtgcacca accaacgctg aaaaagcagc
    12661 tcgatcaggt ggtcgagtcc tgtgtgaacc aggttggtgt cgatgtgaat actgcatcgc
    12721 ccaagctgct tcagtacgtg gccggtgtcg gcgagacgct cgcccaaaac atcgccaact
    12781 atcgcagtca gcacgggccg ttttcatcgc gcaaacagct tctggacgtg ccgcggatgg
    12841 ggccgaaggc gttcgagcag tcggcaggct ttttgcgtat ccgcgaaaat gccgagtcgc
    12901 cgctcgattc ctcggcggtg cacccggaaa gctacgacgt ggtcgagcgg atggccaaag
    12961 acctcagcgt gtcggtccac gagctggtcg ggaacagcga cctgattcgg caaatccaga
    13021 tcgttcgcta cgtggatgat cgccggggtg aaccgacgct gcgcgacatc ttggcggaac
    13081 tggaaaagcc ggggcgcgat ccgcggcaag agttcgagga agttggcttc aaccccgacg
    13141 tgaccgaggt gtcgcacctg aaagagggca tggtcctaaa cggggttgtc accaacgtca
    13201 ccaacttcgg cgcgttcgtc gatgttggtg tgcaccaaga cggcctggtc catatctcgg
    13261 agctgtcgca caagtttgtc aaagaccccg ccgaggcagt caaagtcggc gatcgcgtca
    13321 aagtccgcgt catcaaggtc gagctcgacc ggatgcgaat tggtctttcg atcaaagcgg
    13381 cgagcgagcc gccgaagccg gaaccaggcg atgcccgtcg tccgccgcga gcggtggatg
    13441 gcggacagcc gcggggccga gacggcgaca gacggcgcga ggacgggcca cggcgtgatg
    13501 aggggccacg tcgcgaagag gcaccgcggc gtgatgacaa ccgggcccga ccgcccgccg
    13561 cacagagcgg caacgcccga ccgcccgccg acaaccgtcc accccgtcga gacgatgccg
    13621 gccctcgtcc gcccagccgc ggggacaagc cggcggcagg tgggcccaag acctcaccgt
    13681 ttaactcgat ccgcggtctc aacctggtac tcaagaagta gtccaggcga ggccttccgg
    13741 gggggttact tcttgccccc gcccccgatg ttcatcgcca gcgcaatcaa atcctcaatc
    13801 acgagccgcg gaatggatgc gctcatgacc ttggtcatgc cttgcggtcc gatgctgcca
    13861 ccttgcgcgc cgtaggtgat gggcacctcg ccgtggtgcg gaatcacatt gagcagcgag
    13921 gttccggtct gacccggcgc tgcgcctttg ccaagcggcg acttttcggc cacccgcatc
    13981 acggtgtcaa acaggccaat ccagcccgcg agcgtcgtaa agccggcact ttgccagccg
    14041 ggctgctgca cctgggcgag gccgcttcga ctcgccaggg tttcgctggc cggcagattc
    14101 gccagccgct ggagtttctg aagcgcgagg tttttgtcgg cagcgaccgc cgaccacacc
    14161 cgacccgcga cctgcgcact aaaaaggtag acgcgaaatg ggccgcgttt ttggcctttg
    14221 gtggccttgt ccagctcact gctccagccc gatagatcca atgacaggta gagccccgcc
    14281 gcgtcaggcc caaacggctt gccaacggtt tcggcgcgga tgattggaat gggggacttg
    14341 atgccgacgg tcttccactt ctttttcaaa taggcctgaa gccctgggcg attccaggtg
    14401 gcggccagcg cgcgcaggaa ggccggcgac tcatcgacac cgccattttc tcgctccgcg
    14461 gtcgacaggt aatagtggtt gctgagcatg ttggtgagcg gatcgcccgc cgcggctggc
    14521 ttgtcgccga ttccttcacc gagcacggtc gtcatcacgc cgtcccactt gggctgtttg
    14581 agcagcagat cccgaatggc ttggcgatcc gcttcagcca ggccgtcgtg ggccagaaaa
    14641 ccgtcgacga gaggcagcag cagctcaacg acctgcttgc tccaccgcgg atcggtcacg
    14701 tagtacgacg cggagacgac ttctttgggc agggccgaaa acgctgcggg cggcgcgatg
    14761 ggcttgcggg ccgccgccgc tgcatctgct ttggcccagg ccgactgggt tcccgacatt
    14821 ttgtagccca acgcgacctg gacctgatcg gggcgcagcg tcagatcgag cgcgagcagc
    14881 gacagatcct gtccggtcat cgcaacttgc ccgatgagcg cctgcactgc gtccgtcgct
    14941 gcgcgatcaa actttggatc gccaatcgcc agccgctgcg gcagcatcat cgtgccggcg
    15001 tccagcacct gcttccactg cgtaccaaaa attcccagca gcgacgcgac cgacacttcc
    15061 gcatagaggt ctttgccttg ggctccgggg acgttcagat tcgcaagctg aggcgacagc
    15121 atgtcgcgtt ctcgttccga cggtgcgcag gtgagcttgc ccggaccggc ctggccctgc
    15181 agcacgcaat agaggacgtc gtttttgccg agcggaaagc gaagctgggt ttggccgttg
    15241 cggttttccc cgagcagccc ttgggatttg gcgatcttgc gggtttcttc ggcgctggtt
    15301 gtggataaag ccaccgccca cggtggggct ccgcgcatgg ccgtcgccgc gtcttgttcg
    15361 agtgccagcg cgaagtcgag cggcgccgct tggtttatca ccttggcaaa atcaccgacg
    15421 acctgctgga gactttcctg caccggcagc ggaaaaggaa cgtaacccgc cagcgtcttt
    15481 atcgactgcg acaggttggc gatgcgaacc gtcgcgacga tctgggcttt gcccatggga
    15541 gcctgctggc ccgatgtcgg gttcggctgg gctttggggg cagcttgggg tatcgcgttt
    15601 ggcgggatgt ccgcgacggc aaagggggcg gaaaaaagca tcgtgccgca cagcgcagcg
    15661 gcagtgatgg tgtgcattcg catgtggcta tcttcgcatg gaaccgccgt gctgccagtg
    15721 ctgtcagacc gagctggtaa agccgggata tcgccgctcc aagtcggcgt gtatcatcgt
    15781 ctttttcgtt gagtccggtt ttgcctagaa ggagcacgag accatgaagt tcagcttccg
    15841 aacggtcgac agtcctgcga tggttctcgc ggagaaagca gatcagctgg cgatcccgct
    15901 gggagaaggt tcactgcaaa gtgacgcgct gctgttggcc atcgacgcgc agcttggggg
    15961 aacgctgcgg cacacgatcg acgacgagca gtttttgggc aaggcgtccc agagtctcaa
    16021 tttgcatagc ttggggcggc ttggcgcgac gcggcttctg ctgctgggga cgggcaagcc
    16081 tggggcggtg ttgctggcgg atctgcggac gtggacagca cgcgctgtac ggatcgcggg
    16141 cgcaaaggtg aagcgtctgg cgctgtgtct gtcgggcgtc gcggtcgagc aagctgcgct
    16201 tgtgggtcag gcggtcgccg agggcgcgat tttggcaagc taccagtttg acaagtacgt
    16261 gaccggcgat cgcaagcgat cgtttctgct cgaagaggtg gtggtgttcc tcggggcagg
    16321 ggcatccatc gaagcggcgg aacacggtcg tcgtcgtggc gagatcattt cgggaggcgt
    16381 gattctggcg cgcgatctgg ccaacgaaca gccgacggtg atgtgtccac gcaagctggc
    16441 cgaagtcgcg gcagatgtgg ccgagcgcta cggcctaagc tgtacgattc gcggcgtcaa
    16501 agagtgcgag cagctgggaa tggggatgtt cctggcggtc gggcgtggtt cgacagaaga
    16561 agcccgcttc atccacctga cgtatcgccc gagcactccg ccgcgtcgca aggtggcgct
    16621 gatcggcaag tcggtgacgt ttgattcggg cggtctgtcg atcaaaacca ccgaaggcat
    16681 gctgtggatg aagatggaca tggggggcgg agcggcggtc ctgggggcga tccaggcgat
    16741 tgcacagctt ggcagtgcgg acgaggtgca tgtgctgctg gctgcggcgg aaaacatgcc
    16801 gtccggaaat tcgtacaagc tgggcgatgt gctgcgcagc atgagcggca agacggtcga
    16861 gatcaacaac accgacgccg aaggccggct gacgctgggc gatgcgatga cctatgccgt
    16921 cgaaaagatc ggtgtcgatg agatcatcga tctggcgacg ctgaccggca gctcaatggt
    16981 ggcgcttggc aacttcaccg gcgcactgtt ttcaaacagc gacactttgg ctggtcgtct
    17041 gctcgaagca tcccagcagg gcggcgacga tctgtggcgg atgccgctgc gcgaccggct
    17101 gttcgactcg ctcaagagcg acatcgccga catgaaaaac tcgggcgatc gctatggcgg
    17161 ttcgatcagt gctgcgctct tcctgcgcga gttcatcggc gacaagacgt gggcgcatat
    17221 cgacatggcg ggccccatcc acgccgacaa agacttcggt cacgtctcaa aaggcgcaac
    17281 cggcttcggt gtcgcaaccc tggttgagtt gctgagcccc cgctaaaaat ccccagagcc
    17341 actgctggga cttgaaccca ggacctacgg tttacgaaac cgttgctcta ccgctgagct
    17401 acagtggcga aaagcgaaac gcagtctact tagcttccca acgcacagca agacggattt
    17461 ttggctaggc ctgctttttt cgggtcgtgc gccgctgtga gccttttttg gtcgtcgcgg
    17521 cactcggtgg cttgggcggt ggcctgcgca cgaacgcggt cgcggtttcg tcgatcgatt
    17581 cggatcgccg atcgacgccc ggatcaaact gccgcagata ctgacagcgg ggctcctcga
    17641 cgtgtggcgt gactttgtga tcgttccaca gctgttcttc gaggtacttg tagtttgcgt
    17701 agctttcgac gaaaaaatcg atctcgcgcc gcctgattcc gccacctgtg tcgtctgcgc
    17761 gcacgcagcc atcgtgatag ctgccgtcgg gcaagcgcat gccctttagc tcgggcatat
    17821 acagcggtgt tccgagcggc acccagcgtg gatcgacggc gacggaacga aacggctgaa
    17881 gcgcccgctg ctggccgcct ttgccaaacg gatgcgcctg cgggtcgagc gtttggtagc
    17941 acgttccgat tccgtagtgg cagcgtccgt cgtagttgat aagccgacca tcgcgcagca
    18001 cgccggaccc ttcgagccgc agctcaaaaa caaacgcgcg tgggaaccgc ccgaggggaa
    18061 agccttggcg ggtgtagatt tcgatctcgt aaggctcgtt ggcgtattcc gattcatacg
    18121 cgagccagta gtacgtcagc ttgtaggttc cggtttcacc gagcggatag ccgcagcagc
    18181 ccttttcttt gccactttgg gacagcttgg taaacacgct gtggggctgc ttgctttggc
    18241 cttcgccgtc gggggtgagc gcgcgaagtc gatccgaaga aagcgccgac aggaccgaat
    18301 acggaagtgc gccgctgggc aaaagaccgc tcaccgccga cagtgcaacg ccgtcagcgg
    18361 ttcccggtgt ggccagcaaa aggagcgcaa aggtcggtcc ccacatcgca atcgaagtcc
    18421 ttccgtcctt tcagcataca agcagtgcgg gctgctcaga gcgacagcgg acggaaccca
    18481 aagattcgca ggcgacgcag cagcacgtca atgagcaaaa gcccaatcgc cacccacaag
    18541 agcgacggcc acagcgcccg caggaagcga attttttcat cgcccggatc gagcaccgac
    18601 gtggcgtctt gcagcagttt tccatgaccc agcgtcgcaa cttgcgcaag aagcagatca
    18661 tcgggcggca gcgcgaggta ttcgcgcggg tagggcaaag acagcgttcc ccaagactcg
    18721 gcgacgatgg gtccgggcgt gctcggactg ggcgacggac tgcgatggat ggcgcgcagc
    18781 aaaaacgatc cggtgcgtcc cagcctgacc tcggcttcgt agcgaccggg ggcgaccagc
    18841 gacatttctt tgcgctcgac gatatcgcct ttgccggcga tgagcccaat gccaccctct
    18901 gccggtgcga tcacttccag ttcggtttgt agccccgata ggaaacgatc gtcggcggat
    18961 actgcatcga cccgaacccg caaaagcggc ggtgccacca tcgtctgcaa gtcgtagccg
    19021 tggccgctgc gctgtccatc atggcgcatc gacgaacgaa ccagctgggc ccagaacttc
    19081 gcgtatcccg gccagcgcag ccagtcgacc gcccagcgat tcttggcgtc gctggtgaac
    19141 gcgaccgctt gccccagtcc ttgtcgccac cgggccagca gtggttcacc gagcggcgag
    19201 accaagatca cttcggcgag cggcttggct ttggtcgaaa catagccgcg cagtggcggt
    19261 gcgttgtcga tggaaacgcc gtcgagcagc tcgacaaact tggcgacttt gggcaccacc
    19321 cgttcttcga tcagtgccga gcgggcgacc tgggtcgtct ctttggtgaa aatcttgggc
    19381 accgacgatg catcttgcgt gtagtaaaaa cgcccgccgc cgtgttcggc aatcgcggtc
    19441 agcagcgtct gatccgcgcc ggcacccacc cccaccgtcg aaacggtgat cttgttttgc
    19501 accatctggt cggtcagctc gatgatgcct tggtattcgg cttgtccgtc ggtgagcagg
    19561 atgacgtgct tgatcttggc attggccgac acaagctgct gatacgcttc gttgagcggt
    19621 ggcagaagct gcgtgccgcc cccggcggtg agccgcgcaa tgtcagtcga gattcggatg
    19681 cgattttggg cgcgctgaag ccgcacaagc ggaatggctt ggctgtcgaa agcgaccacg
    19741 ccgatcaggt cgtcgggggc cagcatttct gccgtcgccc gcgcggcatc tttggcaagc
    19801 tctagcttcg ggccattcat cgacgccgaa cgatcgatgc acagcaccat ggccagcgac
    19861 ggctgctcgc gttttttttc ctgctcgaac cgcaccggca gaagtttttc tagctgcgtg
    19921 ccttgataac cgcccgatcc aaagctgttt tcgccaccca ccatcacgaa gccaccgccg
    19981 agatcacgga cgtaggactc gatggccagc atctgggcct gactgaccag cgtcgcggac
    20041 acatcggaca agatcaccag atcgtagggc agaaggtctt tggccgaaga cggcatgccg
    20101 taggggccgc gggcttcgac gtcgatgttt tcgcgctgga gcgcttgcgt cagatagcct
    20161 ttggccgtcg attcgccctc gatgtacaaa acgcgcggct tgcctttggc gagcagtgac
    20221 agttgggcct ggttgttggt cgcgatggtg tcggaaagtg gggtcgccgc ttgcgtcccg
    20281 ctgtttggtt tggcgacgag ccgagcttga taggtgagag cgccggggcc tttgggttcg
    20341 ctgcggaagc gcaccaagtt gcgaccgggc gttagttcga tgtcgcgatg tgcatcgtcg
    20401 ggattggggc tgccgttttg caaaagttcg agacgcgcag tctgctgcac actggaatag
    20461 acttcggctt cgagggtaaa tggggcaccg actttgagct gcgcatcggc accgggtccg
    20521 cccaaaagtc gcagctcgcg caccagcact tcgggtgagt cgctggcttt gaggggaaag
    20581 acgctgacat ggacgcggcg tcgggcggct gcaactgctt caccaagtac gtcgccttct
    20641 gtctgatttc catcggaaaa caggatcgtg cggcgcagcg ttccgggcgc gtggagccca
    20701 tacgaaagct gaagcgcggc ttgcaagttg ctcgcggttg gatcggcatc gtctgaacgg
    20761 gtcgtcggcg cgcgcaaagg tggcaggggc acgcccggcg ccggctgcgg aaagagcctt
    20821 ggctgtccac caaagcggac gatgtgcaga tcgtcgcctc gcgaccgggt ccgttcacgc
    20881 agcgtttcga tctgggccga cacccgcgca agctgcgacg acgacaccga gtccgacaca
    20941 tcgaccagcg cgaccaccga cagcttctgc cggtccccga gcagcgccgg gcgagccagc
    21001 gccgtgctca gcacgatgag cagggaactg cgcaacacaa gctggagcca cagctgcttg
    21061 cgcgacatgt cggtcagcga aaccaagagc cccggaatca gccacggaat ggccgaaacg
    21121 agcagcagcc acagcggttg ttcgacgacc accacccgcg gtccgattcg gatggccaga
    21181 ccgccatcga ccgcgctgcg cagcgattcc cccaacagct cgcgcccgga cgacaaaaac
    21241 gcaggctccc acagatgcag cgcaaacagg ccgtagccaa ctgcggccaa catgcccgaa
    21301 aggagcgcgg cgataagcgt cggaagtagc agtgccagcc gaccgcgccg aaacgccgcg
    21361 acgcaggcca aaagtccgat cagcaagtaa cccgcgctga tcgtaagcag ccaagctttg
    21421 ggcagcgcag cgctcatacc gtccacctgc gatgataggt ccaccactcg cacagcagca
    21481 aaagcagcgc cacgagcaac agatacggcc acagcgtgcg ccgcagcgcg acttgacctg
    21541 catcggggcg gacaagcgtc gtgcgttcga gctgaagttc tgtccgcaaa agcgctgccg
    21601 attccgcgcc gtcggccaag ttcagcgcaa actcgattcc gcgcaaaagc tcgggcacgc
    21661 gcagcgaata gaaaccggcc ttttcaccgt acagcttcac aatcccttcg tggaccggca
    21721 ccatcgtgtg cacatcgcct ggctgggtga gatcggcctt ttgcagctca cgcagcttgg
    21781 tgtcgggacc gcgggttttg gacggattgt cacgcagcgg caaaaacagc gtggttccgg
    21841 tgcggaaact gccgagatcc tcatcgacat cgccggcaaa ccagtcgatt gcgttcatca
    21901 gcagcaccgg aaaggccacc cgcagcggca gatcgctctg acggacatcg aaacccaagg
    21961 cgacggcgcg ccgcatgcca agcggtcctg gctgctcacg cgcaatcaca atcgggtctt
    22021 tgatcatttt ggcgagcgga cgatcgaagg ggcccaagcg gaacaccgac gcgcggctca
    22081 tgttgaggtc ggccagcgac acccagcgaa gcaccggatg ttgttcgtcg acgtccgaca
    22141 gatacggcgc gttgaccgtc ttggcgatcg caaatggact gtcggcgcct tgaggatcga
    22201 ggtaaatcgc atgtgcatca ggcgggcggg gcggcgtaaa cgcatcaaac agaaccgcgt
    22261 cataccgggc cgctttgtcg tcgctgtaat cggatggcgt gatcttgtcg atggccaggt
    22321 gattttcttc accggcactt gcggccagca gcgcgccttc cagaaacaaa ttcccccggg
    22381 tgacgatcag catcttgacc cgccggcgct cgggcagcac cgcgtaagcg tggttgtcga
    22441 gcggcaacag atcgacttca ccaccttcga gcttcagcgt cgcttgcagg tgggttcccg
    22501 cgcccgacag gcttgggtag agcttgcggg tctgttcgcc gggaaccaga tcgagcttct
    22561 ggacatcgac cagctcgcct tcttgccgca gctccagcgt cgcggttttt gcgccaggcg
    22621 gactttgggg cgacggaaaa taggacaact ggagcagcac ttcgtagctc aatcggttgc
    22681 ggcgataccg acgcaccgca aacgaagtga tcgcggcatt gccatcgccc ggtcggagac
    22741 gggcttggct tggcagcggg acaaagcgga catcgagcgc cgcaggtttt ttccgcaggg
    22801 ctgcttcgtc aaatcctccg tcggaaaaca gcacgacctg cgggtgtgga cgaccacgca
    22861 acagggcttc ggccagcagc agtccggcct gcaaatcagc gccggtatcg cgggcttcca
    22921 cttggtgtag ctgcgcgagc agttcgcgtt catcgtcgga aaaaccaccc aaaggcgcgg
    22981 gccggccatc caggccgacg accactgcga gatcgtcgcc atgcaggccg cggatgatct
    23041 gttctgcctc ggcgcgtgcc aggtccagtc gggtcttgcc aggcagcagc ggaacctggg
    23101 cttgcatcga cgccgaggca tcaatcagca gcaccagcga tcgtccttcc gtcgaccgac
    23161 ccagccgcgg atcgccaagt gacagcgtca aaagcccgag gatgacaagc tgcaaaagca
    23221 gcgacaacac ccgcaacagc ttctgccaca ggctctgcgc ttcgctttgc ccgagcactt
    23281 tttgccacag cggtgaaaac ggcacttcca gccgtcgccg tctttgccgc agcacataca
    23341 gcaccgtcag caccgcgctt cccgccgccg caatcggcag aagctggttc cacggcagcc
    23401 caagcagcga aagcggcgtc atctgatgaa gcctccctgg cgcagggctc gcaaggtcag
    23461 ttcgtcgaag ggaacgccgg tttcggcgcg aatgtagctt acgctgcggc ttttgcagaa
    23521 ggcggcgagc gcatcacagt agctttggtg ttcgcgctga taggcctcga taagtgactg
    23581 cgaaagggtc acttcgcgta gctcgccggt ttccatgtcg acgatttcca aatccccccg
    23641 cagcggcgga ttcgattcga tcgggtcgat gatttggatc gccgccggct cgaagcggtg
    23701 gtagcgcaac aggttgaggc cttcttcgta gccgtggtgg tcgtaaaaat ccgacagcac
    23761 aatcgcgatg ccgcggcgct tgatgcgctg cacaaactcg ccaatcgacg ctttgaggtc
    23821 ggtttgaccg tcgggtttta ggttcgcgag aaaatcgaag actttgaaaa tctgaccttt
    23881 gccacgcgac gcgggcagct cggcggacag ccgttcaccc agtccataaa tcgccacgcg
    23941 gtcgaggttt gcgagcccga cataggccag cgctgcggtg atctgcgccg catagccaag
    24001 cttggccatc cgtccgtctg tgccccgtcg caaaagtggc acatctggct tgccgccgag
    24061 ccgcatcgac gccgagcgat cgatgagcag atagatgtgc aagtcctctt cttgttcaaa
    24121 cagacgcaaa agcagccgat ccatccgtcc gtagaccgac caatcgaggt agcgcagatc
    24181 gtcgccagcg gtgtagtcgc ggtgatcggc aaactcgatg cccgatccct gtccccgaga
    24241 gcgtcgctcg gcgcgacttt tccccgcgaa gacttttttg gcgacgacgt gcaggtattc
    24301 cagctgtttg aggaactgct gatcgaagcc cgcttgcgcc gcctgcgtcg atgctgccgc
    24361 cttttccact tggggcctac gaccgcgccg ttccggggat cgcctcgggc gtgcgtgaaa
    24421 tgatcgccgt gatgatatcg tccgtcgaga cgccttcggc ctgaccttca aagttgagga
    24481 tcaagcgatg acgcagcgcc ggtctggcca ctttggcgac atcggcgcat gacacggcaa
    24541 aacgtccgtc gagcagcgcc aggactttgg caccgagcag cagcgcttgt gcaccacgcg
    24601 gcgacgcgcc atagcggaca aagcggcgca ccaagtcatt gcgatcggtc ttttccactt
    24661 tcccgagcgc tttttgcgcc tgctctgcgt cgggatggct gccaatcagg atgcgcactg
    24721 cgtagtcttg gaccgtccgc gcgaccggca cctgccgaac caagctgcga agctccagca
    24781 gctcgggccc gccgagcact tttgtcgcgt gcacgctggt ctggctggtc gtgcgatcga
    24841 ggatcgtatg cagcgcttca ctcgacggaa attcgacccg cagcttgaag aaaaagcgat
    24901 cgagctgcgc ttcgggcagc ggatacgttc cttccatttc cagcgggttc tgcgtcgcga
    24961 gcacgaaaaa cggctggggc aattggtacg tcgttttggc caccgtcacc gtctgctctt
    25021 gcatggtttc gagcagtgcg gactgggttt tcggcgttgc tcggttgatt tcgtcggcca
    25081 gcacgatgtg ggcaaagatg gggccgcgct ggaactcgaa ccgtcgccga ccgctctctt
    25141 cttcgacgag catgttggtg cccaagatgt cagcgggcat caggtcgggc gtaaactgca
    25201 cgcgggaaaa gctcagatcg atggcttcgg cgagcgtccg caccagcatt gttttgccca
    25261 gaccgggtac gccttcgagc agcacatggc cgcccgacag aagcgcaatc accacacctt
    25321 cgacaatttc ttcgtgaccg acgatcacgc gggcaatttc atcgcgcagc cgtcgcacgt
    25381 ctttgcgaaa tcgctggatc tgctgcgccg cctgttcagc cgtcgctggt tctgccactt
    25441 gtgacatgta cacaccttcc cgcccggcga gtttctgcca attgtatccg agtcttcggc
    25501 gacccacgca gcgggtgcga acaatttgcc gtcagtcagg ggtttttcaa aaaaactgcg
    25561 ccagcgagtg agacagggga gggcggacgg cggctagcga tagtcggtca ggctcttgcg
    25621 agactggttg cgcttcggat catgatcggc ttggcagtcg gcgcacagcg tcacgtaggg
    25681 tcgcaaagac agccgccgct cgtcgatctc ttcttcgcac tcttcgcaca gaccgaccat
    25741 gtcgggctcg cgatccagac gcaaaagcgc ccgatcgatc tgcccgagca gctcgctctg
    25801 ccgtcggttt tgctgcgagg cgagcgtctg catcatttcc gacagcgcct gttcgtcttc
    25861 gtcgctgacg ccgatggtgg tcgcgtcttt gcgattgggt tctatcttcg ccggcccttg
    25921 gtgcacgacc tcgtcacgca gcttttgcag ctgggccttg agcgacagca gggttgggcg
    25981 agccatggct actttttctt ctgcgacgct ttcgagagca gatcgccgag cgtaccgaag
    26041 ccgacccgtc caccaccgct cggtgccgac ggggctgctt ctccgccttc tacatcggtt
    26101 ggcgaagcca tgccgagcga gagccgccgc ttgtcgcgat cgatctgctg aatcacgact
    26161 tcgatcttgg cacccggctg gaccgcttct ttggcgtgac gcagtcgctt gccgccgccg
    26221 agctcgctga tgtggaccag tccttcgatg ccggggacca gctcgacgat cgcgccaaac
    26281 gcttcggtgc gaagcacagt ggccgggaag cgcgtgccta ccgggaactg ggtttcgatg
    26341 tcgctccacg gatctttttc gagcgacttg agcgagagcg agatgcgctc ggggcgcttg
    26401 ggatcttggc tcggctcgat cttcaggatc gcaacttcca gttcttgccc gacggacaga
    26461 acatcttttg gcaagcgtgc gcgctggaac gaaagctcgc tgacgtggag catgccttca
    26521 atgccaccga gatcgataaa cgcgccgtag tccttgagcg ccgtgacgcg gcctcgggcg
    26581 accacaccgg gggccagctt cttgcgcagc tcttcggcca gggcttgggc ggcttcttca
    26641 agaagcgcgc ggcgcgacag caccacgttc accgatcggc cattttcctc gaagcgggtg
    26701 atgcgaaact ggagcttttg gccgacgtaa ccgctcgcgt cttcggtgtg gcgcagatcc
    26761 agttgcgaga tcggacagaa gccgcggacg cctgcgactt gaacttcgac gccgcctttg
    26821 accacgccgc tgaccgtacc ctcgaacgga atgcccagtt caaaggcttg ccgcagctcg
    26881 ccgcgggcat cggggccacg accgagtgtg cgacgtagca ccacgccacc accacggcca
    26941 tcaatttcga cgatgcgggc ggtgatttca tccccgactt gggcagtcaa ttgcccatcg
    27001 ggaccgcgca gctcgtcgag cgggatggcg ccatcgtcct tgccaccgag atcgacaaac
    27061 gccgaatcac gaccgatcga aaggatgcgg ccacggactt ccgcgccgac tttgggcccc
    27121 tcttgcttgc gacgggctgg cgaggacttt tgctcttttt caaattcggc cagcatcgcg
    27181 gcgaattccg acgacgcagc cgtgttggtt ggggatttct tggtctggga catgggcgcg
    27241 gattctagcg acaccgcttc gcccatgcaa aggtcttccg tgctgtgatg cggacttgcg
    27301 tgctacgcgc gtcctgcgcc ccggcgccgc gaaagcagca ggagcgaaag caaaagacct
    27361 gatgaaagcc acgaaagtcg gctggcagaa gatgcggttt ggctcacatc gcaaccgatt
    27421 ccgccggcac cgagactgag caccggttgg ggatcgggcg actcgcaagc gcccgtaaac
    27481 ggatgacaga ctgtgccttg cgggcaggtg acgtagttac acaggttgtc tacgcactgg
    27541 ccatcggttg caccggcaaa acagccctga ccctggtggc agatggtgtt tttgcagagc
    27601 ggctcgtcga cgcatgtacc gaagctgctg tcggggatcg gctggcactt ttggcctgga
    27661 aggcactggc cgctgcacgg atcgccaacg cacttgccgc gatcgcagcg cgaggtctgg
    27721 gggcagggcg aacggcaggc gtcgacacac tccgcgttgc ttgtgccgtt tgcgtaaaac
    27781 gtcggcaggc agtactgcga cgaaggacac gttttcgtcg cgcaaggatt ggctcggcag
    27841 gaattctgat aacagacctg accaccgggg cagcctttgg catagcagcc gtcttcacag
    27901 gttgcgatgg ttcccgcgtc accgccgacg accgacttga cgatggtcgc gagcgcttgt
    27961 tcgagtgccg tggcgtcttc ggcgcggtag tagtgatagg ttgggtcttg ggtaagcggg
    28021 aaaccgccag catcggccat caggttgagc gacgctcgtt cgctgtcgag tgttttgcca
    28081 aagccgagca ccatcgtata aaccggcggt gttcctgcaa ccgtcggatt ggccgcggcc
    28141 cggatcgcat cgaccgtggt tttgaccgga tcggcaccgc agcagagtgc atctggcgaa
    28201 ccatcggtca cgagcagcaa gtaacctgga cgggtggggt cgcttttcac cagatcagcc
    28261 gctgcactcg tgactgccgt gcaggtcggc gtgtttccac ccgaaaacgg atacgtgatc
    28321 atcgcgttgt tgatcgcgat gcggttgctg tagcccggcg ggatcgggat tttggcgttg
    28381 accgaacagc cagtggggtt tggaaagttg cagtacccga gcggcagcag cccgtcgtac
    28441 ttgttgttca tgttggtgag cgccttggtg gcgatcgacc acttggaatc gggtgatccc
    28501 tgcggcacgg actgaccgag cgagttttgc gccatcgagg cggattggtc gatcagaatc
    28561 accaggttgg gacaggcttt ggcgagcgcg gtgcgcggca cagaaagcgc aatgagcact
    28621 gcagcacaca ttgtcaccgc cgtcgtgcgc tgccaaagtt tgtgaaaaac agtctgagcc
    28681 atagaagtat ctgcctccgt gtagggcgtg gccacttggg cgcgggcggc tgagctaagt
    28741 caggaaaatc cgctctccat catatcgc
//
LOCUS       DAORGN010000044        28724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_622_length_28724_cov_6.380829, whole genome shotgun sequence.
ACCESSION   DAORGN010000044 DAORGN010000000
VERSION     DAORGN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28724
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_28724_cov_6.380829"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKL17_07195"
     CDS             <1..376
                     /locus_tag="PKL17_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNK44549.1"
                     /translation="FSGKDPSKVDRSACYYARYIAKNIVAAGLASRCEVQVAYAIGVA
                     APVSIHVTTFGTGRVSDESLEKLVRAHFDCRPAALIRELDLLRPIYKPTAAYGHFGRS
                     EASFSWEKTNKAAALKDAAKAA"
     gene            466..1089
                     /locus_tag="PKL17_07200"
     CDS             466..1089
                     /locus_tag="PKL17_07200"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNK44550.1"
                     /translation="MARIVTHAEVANIPDGGELSIEPGAELTPLAKERATARKIRITV
                     GTPRMNEDTMAVAARVAEHVLMRLGKSDASVVERVASEVLAALDPGTQSSAAPGLPPA
                     ADYCSAYIESERSRARRKVVITSTGRNQKGVVAKLTAILAELGGDILDISQTLVGDYF
                     TMLVIVDIAEMRVDFSQLKTQLQQVADAMGIHVMLMHEDLVRSLHRV"
     gene            1109..2488
                     /locus_tag="PKL17_07205"
     CDS             1109..2488
                     /locus_tag="PKL17_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNK44551.1"
                     /translation="MIFDLEETRAILRMIETEHLDIRTVTLGISLRGCATADLGETCR
                     RVYDHIMRSAERLVPVAKEVNAAYGVPIVNKRIAVTPIALVGEPSGAASYVPLAQALD
                     RAAEQLGIDYIAGFSAIVEKGITSGDAVLLDSIPEALSTTNRVCSSINVATTRAGINM
                     DAIATMGHVIRALAQSTADRGGIGCAKLVTFANMPEDNPFVAGANHGIGEGDLTLNVG
                     VSGPGVVLSALRRLRESSRKVTLTDVAETIKRMSFKVTRAGELIGQEVARRLGGKVRF
                     GIVDLSLAPTPAIGDSVAEILEILGLERTGCHGTTAALALLTDAVKKGGAMATSSVGG
                     LSGAFIPVSEDHDMVSAVRTGALSLDKLEAMTAVCSVGLDMVAVPGDTSAAMISAIIA
                     DEISIGTFNNKTTAVRIIPVPGKGPGESVMFGGLLGEAIVMPVSRYQPDGFIALGGRI
                     PAPVISLRN"
     gene            complement(2492..5509)
                     /locus_tag="PKL17_07210"
     CDS             complement(2492..5509)
                     /locus_tag="PKL17_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44552.1"
                     /translation="MSKAKTVLEVRILSGGRAGEQQLFFDASEVRIGRHPTADVLLPD
                     ASVSRQHAQIVHQGSEGYAIVDAGSTAGIYDLSTGQKVTRLAIPEKGSIEVALGPPMT
                     SPRCALGMGMALPFGRYLLTGRLGGGGMAEVFLARQTGLGGLFRPVALKLIQPEMFEI
                     VDASSMFLDEARIAAEISHHNVVKIYDVGEQDGVLYLAMEYLRGVTVASLIGQFLRRG
                     ETMPPDLAAAIVAQACAGLHAAHQLRDGNGRLLNVVHRDVSPSNLMVLPEGLVKVIDF
                     GVARADTRLLRKEEGLQGKPAYMSPEQILLQPLDARSDVFALGVVLYEMCSGQPLFAR
                     DDTAATFYAIVRGELTPLRKVCPQASARLEAVVHRALARPLHERYVSAADLAQDLDKV
                     VEESGGRFSSIGAIGRFLQERGIHLQAAPPQLLHEVPKALYGRKPVERSTDFVDEEAI
                     AQETFGPALSGPPKSTMGPVPDGLPPSTAKPPEPPRPDSASPRSSDAVPLWTIDPRRS
                     PGSGRANLALLRPLSEEPHRLAGLVLADRYRLLRFIGSGPKRDQPFPKLYFQAELLSD
                     GPNEVKARETKWLRGGHVAVSVCGRGPQLAPLSSLQHAKLQALTDSRPPRSDSRFLIP
                     MLDCGLLPQREESDRDGSGSSFLVMPLCERRLPTIGQLPTAERLALVERLLLALSELC
                     RQDPRFVHGAIRPSSVGLCSQTDAVVLLDFCIEQVLGEPLLSPSLERPLLASPYLAPE
                     CYGGAPPSPRSDVYSVGVLGYEMLGGDVQLLTQALRSRRGVPRLPPQQGVPPHLENAI
                     LAALRGEPSQRPTAEELLPILKSQPVEAAVSRSASAVPTLLLPPSGEVLRQRLPSGHD
                     LYLSTLPLLSGQQPTMIPLQLVEQPDALPAELQLLLSGDTLQVELGQSGRGRDRPSLY
                     HDAQQPNTRCDCLTLLPTQSQGHFDVGHRRSTVTRIGYVSAQGSPSGGVQDLSLPELG
                     IAVRSSGPLRRLTVLYTASTSRRLFYAVCLCLG"
     gene            5766..13994
                     /locus_tag="PKL17_07215"
     CDS             5766..13994
                     /locus_tag="PKL17_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44553.1"
                     /translation="MAERKEQPASIFPDADALIQKAAGQRGMEAAQSLQRAAELLLKK
                     GEVEQAYSCYERALGELSEQIGSPPAAKLGAALEFELGKLCEEELGRFEQALLHYQQA
                     FKLRPDYLEPLRRGRLIYQSLGDMDMVARLIDLHLSNLVAGEAEQGIALALELGQLKL
                     RLHDPGGAVEVLRSALRMHNEASEEAEVPEQLLATLAESYMSPDYQPGISEKEHARRN
                     ASDIFLALAKRYLDEELLDILEKLRAAEEEEGNTTPLSVEQLVKSKNRPLTDQDKKAL
                     GYLKRALSSDIRNVTAAVLLEAMYLRLPDSVGTPELIKLYRSGARVTRRGPKLLALYE
                     TTGSPDYQAVADACRAGLDAVSSMDEWHETRQVLVDVLKKASDLLGLGQLREEDALEA
                     ALPEERAELMMEAADYYNRGGDTERYIACLKQAFHELPLHSEAFRKLSDYYKSRRDFI
                     GLAVIQENRMAAQFETARLDLQSYAKQLEELGELYEKKLLDVVSAAAIWRRIDELMPS
                     LRSQSERKRLGQRLSRTEMQINELVVELERMDVEDPARVEYLRRLAQLYREIHEPKHA
                     TTVLEQLLQVAPGDLPSIKVLIELAEQSGDTTRQLELLRQQAGIAADKAERLGILRRM
                     MTLCDQRLLRRDEQFDGQDSLEMMVWVCRSLLAELPSDRDALKRLADALQLVGSKNEL
                     LEVMESYLKVAPTPREKLTLHRRIAKIGEDCGDLARAVSHLERAVRICPPGPEAEEVL
                     CELARVYGAQGRMELAVQTLELVQKQSSRSGPELHRLLGRLTLQADDPQLLEKSLRAF
                     REVLVRLPTDTEALAALQKIHRARGEWKELEQVLRKQLEPSDPELPPRERLSLALDLA
                     EVIGQHLGDPRAAAELLEKVQAETPIVDLRVHRHLRGLYEELGDVRRAVACEERELLL
                     TEDPVARVERAIEIARLWQTRSKDHARAYLSYERAVRLMPDLPEGTPERAAVRRLITQ
                     ALEAMSQMAVHRGKWEDVVSIGQKRLNLAVEEGEAMQAAAILVELAQVYEEKLSQPTD
                     GFAMRIQAFELSPDLMSIAELADLAGKYGQWKPLADLHIQRVERALSEKHDVPLDSLL
                     AASQILEQKLGDPIQAFRLAKKALPITVSSAMAATNPRSLQARLLSEMDRVLRALHKK
                     GGEQSSELALESVSLARELAGLYRALVDELVRSTDAGIEETALRIHVLLGESAKVREQ
                     VLFDPAGALGDRMHAFTVGGERDHSEDPQADEVFQFTVAEVHRLALVSGQIKEAVAVD
                     MRRQERAQTDLRRQQVACESAGWLDDFGNDPQRALKACIKALPMCQEGSDAQAEIRGR
                     LHRLGQKMGLLAWDEVARAERALAQNDPKKLRQRLLYLAALWQQGAQDSVRAIDAAGQ
                     ALRLTFFPSGVPTGKSAQPLSELLVPQLSAELVSEQQQIRGVLDSLFRAAQGESEPAA
                     KLVTLLDNLANQLTEAGAPQWAARVMLDAAQADEKRGRFGQAERRYQEASKQSEVQDE
                     AMSGLERIYRQQRRLADLAALLEKRRPLLAETAQLKLLVELAEVYREINKHQQALNAL
                     QQAVAIDDSDAGPHLSMAKLFETQKTFTRAIESYKAAAQRTPSAIDGAKALLQAADLL
                     ERKLNENDEALALTKQALRRTLVEQGKRLSQGELLLPGDPLREPRDAAWNHVERLLGK
                     LQRQEEMDALVDERLEVTPALLHDERESLLSKQLQTLKAKLQTPSEVTPRDGEEAATP
                     SPDLESRRLHLLAELGKLRPTDDTLLDEQQAILTARKELAKAREIAAQRLQLAEKQAA
                     DPSTVSERQLAVANLDIELGQFADAESALEQVLLRSPTSASALTTLVELHTRTGNHGG
                     LVAALVRMSSQQIDLVAAVGALRQAAQVALTAQDDVPAAKKLLGDALVRIDAAPTVTP
                     AQKEQVRRALGQVLVPLFDLARREGNTSFAQELAQRAIHEADLTGTHAADMQVHLGQE
                     ALSAGKSDDAIRFFDAALSASPGLLEPTLALLELLGPSGNHARIDDLIEAALKATSTG
                     ESILPDAERARLLRKQATARQELGRLDGTLDALKAAELIVPGSLNDQIWLGETAFAQG
                     DFETAHSQLAKLVRFVAEKDALPAPLSVGQLADLLDHAGQSAAELHKAEEARSLWQAA
                     LQVVPDHSSVMNHYLELLLTAGQSQDAEQALSMLTTRASQAAAAGDTAGARRDIRRAA
                     ALSAGPLGDNLKAQEFLRQVLTMLPPLDDSSDAALRDEWTTLLSQVYDNAEKLGELAE
                     ARGYAERLAQLSETVKGQSVWLHRAALCALRCGDSAGAKTLVLSALAATPAQLGLIGD
                     YLEQASDDEANEKLPVLLDAAAATENSATTPKGPDWRDQLKTLWVRAAEIHVRKGDGQ
                     KAAQAYERALSTVADLSGNETGTLRRALLEVLPDTEHDRARTHLHALLKSAPRDVSLL
                     SKLQSVEERAQNPGAAWRLAQLLHLLDANLPAPPLPSLPPKDIKFDEADHQRLADPEA
                     RAMADVLSSLWDGVFSLKAPTLDALGVQGTDRLQGNENSPDELARAFALCSRVLGNQR
                     GGLYRPAGHALALPRLMAKLPTAVIASPQIGKRPPGELVFLLARAVESLRPEYIMPVA
                     MPATDLNRLLQLAVKAFSPRQTAKPTDDVTTWKRELAYRASKKLQDLLKEQPDLDPAA
                     TGYRLAIRRAIGRAALLVSGDLLAARNVLLQIDVPAAQDAGSTAAGYYLRLGEGESSA
                     LADAESDLADLCAFFIAPQNAALFDKLHPR"
     gene            14074..14691
                     /locus_tag="PKL17_07220"
     CDS             14074..14691
                     /locus_tag="PKL17_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44554.1"
                     /translation="MNTKPSLVRPAGFSSFLLGLLVSVSLLGGCREPFDGFAPRTDGS
                     SDLSTISDASVRDGATVYDLTSKADLAWPAPSLPPSSCYQRSAGVTGIQVVLRVDQYI
                     GVRTGRNGPHEIVHGTIINTPWVYQASIVDTTNVEVAMNIQTATDPTGLPMEIPVRAR
                     DQLELEGEYIPKSTANATTALGPSAVIHFTHSPCGYAVLGGTKYK"
     gene            complement(14702..15895)
                     /locus_tag="PKL17_07225"
     CDS             complement(14702..15895)
                     /locus_tag="PKL17_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNK44555.1"
                     /translation="MPLPSRLFLSAVLLGLGCQSPEPTSTLLNEVPLAVHSVSWNAKN
                     VATGKISAVAELNEDTVVFSDQGALVFTAGLLLNTDATSKSWNAASVIPAGDLAGQWL
                     VGVDGKGHLRRLRDRAVLEDVSDRYGLRSDVISDLAAADVATATVFALENELAVADGK
                     NVVRYPVKLRNLQGGGGRVVGISDGTAIAFEPSSGKLKRFGLPQAAVATVIDTAGRVV
                     IATKDTLYREQSRELGSALDVLYVSADAPLLGLALASGEVWVQVGDALALLASDAIQK
                     SPPLALPADAKLFGSPTGDVWVLSSGMLRRFGEDSGGGADEEVWRQTVQPVFTRLCAL
                     CHMPGGSANLDLSTYKSWDIRRAAIKQRVIDGKPTPMPPKGAGTPTQDELMAISAWLQ
                     PKMKP"
     gene            complement(15905..16831)
                     /locus_tag="PKL17_07230"
     CDS             complement(15905..16831)
                     /locus_tag="PKL17_07230"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNK44556.1"
                     /translation="MTLSVTPRVKEILSYYPSDNPGTLSNLARLLMHGRLAGTGRMVI
                     LPVDQGFEHGPYRSFGMNPDALDPDYHFRLAIESGCAAYAAPLGFLEAGAGKFAGEIP
                     LILKLNNSDSLSKLEPCSALTGTVKDALRLGCVGIGFTIYPGSSSRNEMYQQLRDLTA
                     EAKEAGLIVVTWSYPRGANLSKDGETSLDVAGYAAHIAAQLGAHIVKVKLPKDRIEQP
                     EAAKVYGAHKPASATMAERVRHVVKCCFDGKRVIIFSGGETKDNDDAVLDDIRAIRDG
                     GGYGSIIGRNAFQRPFAQGKKLLTDIMDIYAK"
     gene            17043..17255
                     /locus_tag="PKL17_07235"
     CDS             17043..17255
                     /locus_tag="PKL17_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNK44557.1"
                     /translation="MWLQAANLSYLGIFFGVSIVIGAALGSFLDKKWGTHPYLLMVGV
                     VLGIATGFNELWRVAKRYQKQLKQKS"
     gene            17252..17680
                     /locus_tag="PKL17_07240"
     CDS             17252..17680
                     /locus_tag="PKL17_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44558.1"
                     /translation="MNDQTQTPAPPVNTPVRAHFRAVERITLVLGALLVVASMLLVSL
                     KLQLSVSYGVLLSIVNARAVRFFGERLAARQSVGLVVVLFQLKLAVLGVLIYSGLKLF
                     SLDGIGLCVGLSLLPLSIVVRAVQWGMSAPASNGELVSNG"
     gene            17673..18521
                     /gene="atpB"
                     /locus_tag="PKL17_07245"
     CDS             17673..18521
                     /gene="atpB"
                     /locus_tag="PKL17_07245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNK44559.1"
                     /translation="MGEESTWFDFLPGLANLIAWAKQYLGREDPSQAFFFQGGPFVDS
                     HFNLTHVFSALLVLCFVTLGAMSFASAMRKGGDDAIVPPSQFSLRNLFEVVGDALWNL
                     TRNIMGEENGRKYLPLIGTLACFIFFSNVLSLIPGMVPPTSTLKTNVALALTVFVITH
                     VEGIRAQGIVSYLKHFAGPIWWLAWIMIPIEIIGHLARPLSLSMRLMGNMVSDHKVVG
                     VFFFLVPLLVPVPFLILGTMVAVIQTLVFCLLSMVYINMAVATHDHDHDEHAGHDGHG
                     EHAHAH"
     gene            18676..19017
                     /locus_tag="PKL17_07250"
     CDS             18676..19017
                     /locus_tag="PKL17_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNK44560.1"
                     /translation="MTTKISKYMLAAMAALVTLMATSAAYAQAASPEVAKAENARWYA
                     LAAALGLGIAAFGGALGQGKAAAAALDGIARNPGASDKLFTPMILGLALIESLVIYSL
                     LIAFLLYGKVS"
     gene            complement(19044..19811)
                     /locus_tag="PKL17_07255"
     CDS             complement(19044..19811)
                     /locus_tag="PKL17_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNK44561.1"
                     /translation="MVKIEKQAAVWTVIVDRPRVRNAVDRETAHALTQAFRDFDSDGE
                     ARVAVLYGEGGHFCAGADLKAVAGGSPNQLTVDGDGPMGPTRLRLGKPTIAAISGYAV
                     AGGLELACFCDLRVADDTAVLGVFCRRFGVPLIDGGTIRLPQIIGLGRALDLILTGRP
                     VSATEALSMGLVNRVVAAGTARQNAEKLAQELARLPQSCLRSDRMSTLLQYDDPLPVH
                     EALQREFQLGMQTILSGETVGGATTFASGAGRHGQAL"
     gene            19926..20615
                     /locus_tag="PKL17_07260"
     CDS             19926..20615
                     /locus_tag="PKL17_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44562.1"
                     /translation="MFAKGSIFSFALCTLLSISVPVSAAPPPSPEAKAIARAAVEAAE
                     LSPQKTRSLRLRLHLSSLLPQLRVTFGRGWQLSSSRDYLLETPATENDRINYAITASW
                     DLSRLVVPHEELHLIKEEQRRATLRLRLEERLARLLAARCRLQKPEHPTDRTKLDELE
                     ATLIVLTGGKTLPAVDEKQDCPAAPLRIGLTASANTPASADDVLAAPAHDGTAAHESA
                     EPSEPASSPDE"
     gene            20645..21043
                     /locus_tag="PKL17_07265"
     CDS             20645..21043
                     /locus_tag="PKL17_07265"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNK44563.1"
                     /translation="MIVGVGLDLCLVSRMEKAITRSGGRFAARVFTSDERRYCESRAQ
                     PAQHFAARFAAKEAILKALGVPEGLSWHELEIAQRPSGGPQVVLHGVARQAALALGIS
                     VLHLSLTHQGDAACAVVIAERWAEASERSL"
     gene            21043..22704
                     /locus_tag="PKL17_07270"
     CDS             21043..22704
                     /locus_tag="PKL17_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNK44564.1"
                     /translation="MIPLVTATQMRALDEETIAQLGVTGGVLMETAGRGVISVLFDLH
                     RQGRFDLRTAQVCVVAGPGNNGGDGMVIARYLHSHGIACDLWIVALRPRIRGDALVHL
                     AAAEAVGVTSHFCEAESPLLQQRLAALSPGDVLVDALLGTGVSRTVGGGLCTVITQIN
                     ASRAITVSVDLPSGLDADRGIPIDAGPAPVIVRADYTVTFGFPKLGLCGSPGFVFAGE
                     TFVADIGIPAARCAHHGVTAQLVSAQVMTPLQSVRPPLGHKGSHGHLLCVAGSEGKLG
                     AALLATEAALRTGVGLCTLAAARAALDGSLGSRCPEAMTLPYDPQDKHALDQILSALP
                     GKTAVAAGPGLLPTQATQELIIRLLAADTLPMVLDAEALNQLVGTLDVVRQRSQRGGE
                     TVLTPHPGEAARLLGTTSAAVQADRTCAVRALADKTHAVVLLKGAKTLIARSHASDTT
                     GVTLAMIPTGNAAMGSGGMGDVLTGMIGALLASGMHAFDAACLAAYWHGLAGDVAATR
                     KAPGSIVLATEVMQDLDEARKVGGTFASSQPSWPVWPLAWAASRT"
     gene            complement(22701..24020)
                     /locus_tag="PKL17_07275"
     CDS             complement(22701..24020)
                     /locus_tag="PKL17_07275"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNK44565.1"
                     /translation="MSKNTLTITDNRTGKTYEVPITDGTIRTVDLRQIKTDSEDFGLM
                     GYDPAYLNTASCRSAITLIDGDKGILRYRGYPIEQLAEKSSYLEVAYLLFHGELPDQA
                     QLSKWIDQITTHTFVHENVKTVIEGFRYDAHPMGMFLATIGALSTFYPEAKHVYDKNI
                     RMLNVVRLLAKVPTIAAWSFRHSQGFPFVYPDNGLSFAGNFLAMMKRMSESRYKPNPV
                     LERALDVLFILHADHEQNCSTSVMRSTGSSQVDPYSAVAAASAALYGPLHGGANEAVL
                     RMLAEIGSLANVPAFVKEVKEGKGEKKLMGFGHRVYKNYDPRAKVIKRLAEEVFEVTG
                     RNPLLDIALELERIALQDDYFVKRKLYPNVDFYSGLIYQAMGFPVDMFPVLFAIPRTS
                     GWLAQWCEMLDDSEQKIARPRQIYTGQDRRDYPAERQNPFEFERKRG"
     gene            24281..26617
                     /locus_tag="PKL17_07280"
     CDS             24281..26617
                     /locus_tag="PKL17_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44566.1"
                     /translation="MSSLESDPTSPAPAFATSPPLPNAARYAFLSRPGPALACLLLFL
                     GLLLVPGLTSFGLWDPHEVRLVEGASEPIAAAELWKPANAIKPRLPLLPIKLGLSLFG
                     VGELGARLPMLAIGIAVAAAVLLYFAIQGRVRAGLLSGVVLLTTPALFLGARQASLHL
                     LPMLGQLLAVTGLAMLCWPQAGKTVVSSVVGLLLAAAGLGLGLLTSGSLIGMAVPALA
                     VGLTLALTDGPILGEMLATAVAGLALTMPLRQILVVSQLRSSLVVGLGVTLLLLGIVL
                     ALLSRRSIWLCASLVGVALLPALPQSLSGYSGWLAGVPHWPAHRDMQADSLVKTLGFV
                     MFPWVALVPVALAQLFLADTESPSSVESAKSGEALRAAFPNLLLLTWLSVGYLLHTLQ
                     AALVQEQSFAALPSLALLVGGYLDGQLHNRRSSIVAAVCSGLLAVFVARDFFFGPEQY
                     LSAQLTELLKWPAVPAGTDVLVGQGGQIVSTFGMLVALAFAIGLYVPSRRAIVLLPAA
                     ATVLALFAMHGLVPAVTRHVSYRGIYTKYEKLGGRELGLYGIQKSSARIYGQNTIEIP
                     SLPELFTYLTKDAQKRAFAIVGASEMAALDVHSFQNNLPYYVVDDSNSQYVLLSNQLG
                     PGEKDLNPLRRLVSTQPPQVQAELHATFDDKIELIGYDIPQEAQRGTEFIVRLHYRVL
                     APVLGNYRVFLHFDGSSARINGDHIPLGGKYPTNYWTRGQYITDEYRMSTSRLNQSAG
                     YYTVFTGFWPGGDGARMKVTQGPHEPDNRVRLSAVRVK"
     gene            26617..27471
                     /locus_tag="PKL17_07285"
     CDS             26617..27471
                     /locus_tag="PKL17_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK44567.1"
                     /translation="MDLKSLADVELRDATFPLSRSAPSNFLPALRTPVILRELVALGV
                     LDPGLSKVIVQNGRVVGCCLVEVGAQGAYISGLGTDALSQQRGGSRALIEAVIAAGKQ
                     AQLGRLSVEVSEADAGPQGLWQAVGFVPLRTRLRMALLGTPNRALLPDPDDRACMAAT
                     RISAVPCGDALGFLQHHGPSQLGAAVSPAVLGKLEKRLSAAAMLVGSAIEAVVIVDKE
                     RKLLVALAGDPAYLPQLAVYAAARLSAAYVDSLADDDPAVEALESAGFSRIAVRTELS
                     LSLPNASA"
     gene            27485..>28724
                     /locus_tag="PKL17_07290"
     CDS             27485..>28724
                     /locus_tag="PKL17_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNK44568.1"
                     /translation="MDTFLRGRPIASCLALLLLLLFPLGGRADGDGGDSGEGDTPRRA
                     LERFLGNVASGRYSEAADDLELHTSEQSDRTKLAKMLGAVIDRRLSADPEWLSRVSDA
                     PSGDQKDGIAQDQDEVGRIPATPIAEPVRLRRVWMRDRSEYRWQFSHKTVQRVPSWYG
                     RLDDVWLREHLPDKLLRKGPKGFVVWEWLALPVVLTASGFVAWLLTLLLELSLRPLAR
                     RSASRWDDLLFRRLRRPVRLLLAVVFWGLSMPYVLITGQAERIVHNFARVGFLLGLFF
                     CVWRCVDVAVQIVRESDWLKSHPAAKGVIPLGYRLAEITVAAIAVVTTLQELGYPVTS
                     LVAGLGIGGLAVALAAQKTVEHVFGGVMLSVDQPMRVGDLVRVDDVVGYVEQIGLRST
                     AIRTLERSLVNFPNGKLADMK"
BASE COUNT         5499 a         8805 c         9133 g         5287 t
ORIGIN      
        1 gttttcgggc aaagatccca gcaaagtcga tcgctcggct tgctactacg cgcgctacat
       61 cgccaaaaac atcgtcgctg cgggtcttgc gagccgctgc gaagtccagg tcgcttacgc
      121 aattggcgtc gccgccccgg tcagcatcca cgtcaccacg ttcggcaccg gcagggtttc
      181 cgacgagtcg ctggaaaaac tggtccgcgc gcacttcgac tgccgtccgg cggcgctgat
      241 ccgagagctg gatctgctcc gtccgatcta caagccgacg gcagcttacg gacactttgg
      301 ccgcagcgaa gcgtcttttt cgtgggaaaa gacgaacaaa gccgccgcgc tcaaagacgc
      361 cgccaaagca gcctaagaac ggctgggcaa aacccactgc cacgttcgat tccctcccgt
      421 cgagactccc aaaagcggtc gcgctgaccg cgaaggcaaa catccatggc acgcatcgtc
      481 acgcatgctg aagtcgccaa cattcccgac ggcggcgagc tgagcattga gcctggcgcc
      541 gagcttacgc cgctggccaa agaacgcgcg accgctcgca agatccgaat caccgtcggt
      601 acgccccgca tgaacgaaga caccatggcg gtggcggcgc gggtcgccga gcatgtgctg
      661 atgcggcttg gcaaaagcga tgcatccgtc gtcgagcggg ttgcaagtga agtgctggcg
      721 gcgctcgatc cagggacgca gtcaagtgcc gctccggggc tgccgcctgc cgccgattac
      781 tgctcggcgt atatcgagtc ggaacgcagt cgagctcgcc gcaaagtggt gattacgtcg
      841 acggggcgaa accaaaaagg cgtcgtcgcc aagctgacgg cgattttggc agagctgggc
      901 ggggacattc tcgatatctc acagacgctc gtcggggact actttacgat gctggtgatc
      961 gtcgacatcg ctgaaatgcg cgtcgatttt tcgcagctca aaacccagct tcagcaggtc
     1021 gccgacgcga tgggcatcca tgtgatgctg atgcacgagg atctggtgcg ctcgctgcac
     1081 cgcgtctgac ggaaacgagg agctttcgat gatcttcgac ttagaggaga cgcgcgccat
     1141 cctgcgaatg atcgagacgg aacatctcga cattcgcacc gtcacactcg ggatcagctt
     1201 gcgcggctgt gcgacggcgg atcttggcga aacgtgtcgg cgcgtctacg accacatcat
     1261 gcgcagtgcc gagaggctgg ttcccgtcgc caaagaagtc aacgcggcgt atggcgtgcc
     1321 gatcgtcaat aaaagaatcg ctgtcactcc gattgcgctc gtcggtgaac caagcggcgc
     1381 agcgagctac gtgcctctgg cccaggcgct cgatcgcgcg gcggaacagc tcggcatcga
     1441 ctacatcgct ggcttctcgg ccatcgtcga aaaaggcatt acgtcgggcg acgcggtgct
     1501 acttgactcc attcccgagg cactgtcgac gacaaaccga gtctgtagct cgatcaacgt
     1561 cgcgacgacc cgcgcaggca tcaacatgga cgcaattgcg acgatgggcc atgtgattcg
     1621 cgcactcgcc caatcgacgg cggatcgcgg cggcatcggc tgcgccaagc tggtcacttt
     1681 cgcgaacatg cccgaagaca atccgttcgt cgctggtgca aaccacggca tcggcgaagg
     1741 cgatctgacg ctcaatgtcg gtgtcagcgg tcccggcgtg gtgctttcag cgctgcgccg
     1801 gctgcgcgaa tcgagccgca aagtcacact gaccgacgtg gctgagacga tcaagcgaat
     1861 gtccttcaag gtcacgcgcg ccggggagct gatcggccaa gaagtcgcgc gtcgactcgg
     1921 cggaaaagtt cgctttggca tcgtcgatct gtcgctggcc ccgacgcccg cgattggtga
     1981 cagcgtcgcc gaaattctcg aaatcctggg tctggaacgg acgggctgcc atggcacgac
     2041 ggcagcgctg gcgctcttga ccgatgcggt gaaaaaaggc ggcgcgatgg ccaccagcag
     2101 cgtcggaggt ctttcaggcg cgttcattcc ggtgtccgaa gatcacgaca tggtatcggc
     2161 ggtccgcacg ggtgcgctct cactcgacaa gctggaagcg atgacggcgg tttgttcggt
     2221 cggactcgac atggtggcgg tgccgggcga cacctctgca gcgatgattt ccgcgatcat
     2281 tgccgacgag atttcgatcg gcacgttcaa caacaagacg acagcggtgc gcatcatccc
     2341 ggtgcccggc aaaggccccg gtgagtcggt gatgttcggc ggtctcttgg gcgaagcgat
     2401 cgtgatgccg gtgtcgcgct atcagcccga tggcttcatt gcgctcggcg gacgaattcc
     2461 agcgccggtg atctcgctgc gcaactaacc gtcagcccag gcacagacag acggcgtaaa
     2521 acaggcggcg gctggtcgac gcggtgtaaa gcaccgtcag acggcgcagc ggtccagatg
     2581 atcgcacggc aattcccagc tcgggcagcg acagatcctg cacaccgccc gacggggaac
     2641 cctgggccga cacatagcca atgcgcgtga cggtgctgcg tcgatgtccg acatcgaagt
     2701 gcccctgcga ctgcgtcggc aaaagcgtca ggcagtcgca gcgcgtgttc ggctgctgcg
     2761 catcgtgata aaggctcgga cgatcgcggc ctcggcccga ctgccccagc tcgacctgaa
     2821 gggtgtcgcc gctgagcaac agttgcagtt ccgctggcag cgcgtcgggc tgttcgacaa
     2881 gctggagcgg aatcatcgtc ggctgctgac ctgacaagag cggcagcgtc gacaaataca
     2941 gatcgtgtcc gcttggcaat cgctgtctca gcacttctcc cgaaggcggc agcaacagcg
     3001 tcggcactgc cgaagcggat cgcgaaaccg cggcttcgac gggctgactc ttcaagatcg
     3061 gcaggagctc ttctgccgtc ggacgctggc tgggttcacc ccgcaaggct gccaaaatcg
     3121 cattttccag atgcggcggc accccttgct gcggtggcaa acgcggcacg ccccgtcgag
     3181 aacgcagcgc ctgggtcaag agctgcacgt cgccaccgag catttcatag ccaagcacac
     3241 cgacggaata gacatcgctg cgcggacttg gcggcgcgcc gccatagcac tccggcgcaa
     3301 gatacgggga tgccagaagt ggtcgttcca aggacggcga cagaagcggt tcacccagca
     3361 cttgttcgat acaaaaatcg agcaagacga ccgcgtcggt ctgagagcaa agtcccacac
     3421 tcgaaggtcg aatcgcccca tggacaaagc gcgggtcctg acggcacagc tccgaaagcg
     3481 cgagaagcag ccgctcgacc aacgccagtc gctctgccgt cggaagctgc ccaatcgtcg
     3541 gcagccgccg ctcacacagc ggcatcacca aaaatgaact gccgctgcca tcgcgatcgc
     3601 tttcctctcg ctggggcaga agtccgcagt ccagcatcgg tatcagaaaa cgcgaatcgc
     3661 tgcgcggtgg acgactgtcg gttagcgcct ggagcttggc atgctgaagc gacgaaagcg
     3721 gcgcaagctg cggcccacga ccacagaccg acaccgcgac atgtccgccc cgcagccact
     3781 tggtttcgcg cgctttgact tcgttcggcc cgtcggaaag tagctcggct tgaaagtaca
     3841 gcttgggaaa cggctgatcg cgttttggcc ccgacccgat gaagcgcagc agtcggtagc
     3901 gatcagccag tacaagccct gccaggcgat gcggctcttc ggacagcggt cgcaagagcg
     3961 ccaggttggc ccgtcccgaa cccggactgc gacgcggatc gatcgtccac agcggcaccg
     4021 catcgctcga tcgaggggaa gccgaatcgg gccgcggcgg ctcgggaggc ttcgcggtgc
     4081 tcggcggcag tccatcggga accggaccca tcgtgctctt gggcggaccg ctgagcgctg
     4141 ggccaaatgt ctcctgcgcg atcgcttcct catcgacaaa gtcggtgctg cgttcgacgg
     4201 gcttgcgtcc gtacagcgct ttgggaactt cgtgcaaaag ctgtggcggt gccgcctgaa
     4261 gatgtatccc gcgctcttgc aaaaatcgac caatcgcacc gatgctggaa aagcgccccc
     4321 cggattcctc gacgactttg tcgaggtctt gcgccaaatc cgccgcgctc acatagcgtt
     4381 cgtgaagcgg tcgcgcaagc gcccgatgca ccaccgcttc gagccgcgcc gaagcctgcg
     4441 gacagacctt gcgcagcggc gtaagctcgc cccggacaat tgcgtaaaac gtcgccgcag
     4501 tatcgtcgcg ggcaaacagc ggctggccac tgcacatttc gtacagcacc accccgagcg
     4561 caaacacatc cgagcgcgca tcgagcggct gcagcagaat ctgctctggc gacatgtagg
     4621 ccggcttgcc ttgcagaccc tcttctttgc gcaaaagccg cgtgtcggcc cgcgcaacgc
     4681 caaaatcgat gactttgacc aagccttcgg gcagcaccat caaattcgat ggcgacacat
     4741 cgcgatgaac gacattgagc agccgtccgt tgccatcgcg cagctggtgt gccgcgtgaa
     4801 gtcccgcaca agcctgggcg acgatcgctg ctgccaaatc tgggggcatc gtctcgccgc
     4861 gacggagaaa ttgcccaatc aggctggcga cggtgacacc gcgcagatac tccatcgcca
     4921 ggtacagcac gccgtcttgt tcgccgacgt cgtaaatttt gaccacgttg tggtggctga
     4981 tttccgccgc gattcgcgct tcatccagaa acatcgatga ggcatcgacg atctcgaaca
     5041 tctcgggctg gatcagcttg agcgcgacgg gccgaaacag tccgccaagt ccagtttggc
     5101 gggccaaaaa gacctcggcc atgccgccac caccgagccg ccccgtcagc aaatagcgac
     5161 caaacggcag cgccatgccc atcccgagcg cacagcgcgg tgacgtcatc ggcggaccaa
     5221 gcgcgacctc gatggagcct ttttccggaa tggcaagccg cgtgaccttc tgtcccgtcg
     5281 acaaatcgta gattccggcg gtactgcccg cgtcgacaat cgcataaccc tcgctgccct
     5341 gatggacaat ttgggcgtgc tggcgcgaca ccgaagcatc gggcaaaagc acatctgccg
     5401 tcggatgacg accgatacgc acctcacttg cgtcgaagaa tagctgctgc tccccggctc
     5461 tgccgccgct caggatgcgg acttcaagaa ccgttttggc tttgctcatc gcaggttggt
     5521 tccacgcggc agtgtatgag agggtacagc accggggcaa ggtctgcggc gtctacccca
     5581 ccccaaacgg cacaactttc tttcagaccg tgccccggaa agccagccac gcgcagtttt
     5641 tcgcaacatt tcaccggcca gaacagcggc aagtcctcat gaaaaaaggg atttgtgggc
     5701 tacagaaaaa aaccgtggta cggttatcgg gcggcagtcc tgaccctgcc gacagagtgt
     5761 ggtctatggc cgaacgtaaa gagcagcccg catccatttt tcccgacgct gacgcgctga
     5821 ttcaaaaggc cgctggccag cgcggaatgg aagccgccca gtccttgcag cgcgccgctg
     5881 agctgttgct aaaaaaaggc gaagtcgaac aggcgtatag ctgttacgag cgcgcactcg
     5941 gcgaactgtc cgagcagatt ggttccccac ctgccgccaa gctgggcgcc gcgctggagt
     6001 tcgagctggg caagctgtgc gaagaagagc tggggcgttt cgagcaagcg cttttgcact
     6061 accagcaggc gttcaagctg cggcccgatt atctggaacc gctgcggcgc ggacggctga
     6121 tttatcagtc gctcggcgac atggacatgg tggcgcggct tatcgacttg cacctgtcga
     6181 atctcgtcgc gggtgaagcc gagcagggca tcgcgctggc gctggaactt ggtcagctca
     6241 agctgcgact gcatgatcca ggtggcgccg tcgaggttct gcgcagcgcg ctgcggatgc
     6301 acaacgaagc gagcgaagaa gccgaggttc cagagcagct tttggcgacg ctggccgagt
     6361 cgtatatgtc gcccgactac cagccgggaa tcagcgaaaa agaacacgca cgccgaaacg
     6421 cgagcgacat ttttttggcg ctggccaagc gatatctcga cgaagaactg ctcgacatac
     6481 tcgaaaaact gcgcgcagcg gaagaagaag aaggcaacac cacgccgctg tccgtcgagc
     6541 agttggtcaa aagcaaaaat cgtccgctca ccgatcagga caaaaaagcg ctcggttatc
     6601 tcaagcgagc gctgtcgtcg gacattcgca atgtcacggc agcggttctg cttgaagcga
     6661 tgtatctgcg cctgcccgat tccgtcggta cgccagagct tatcaagctg tatcgcagcg
     6721 gcgcgcgggt gactcggcgc ggaccgaagc tgcttgcgct gtatgaaacg acgggaagtc
     6781 ccgattatca ggcggtggcc gatgcgtgtc gggccggcct tgatgcggtc agctcgatgg
     6841 acgagtggca cgagacgcgg caagttctcg tcgacgtgct gaagaaagcc agcgatttgc
     6901 tgggtctggg acagctccgc gaagaagacg cgctagaagc ggcactgcca gaagaacgcg
     6961 ccgagctgat gatggaggcc gccgactact acaaccgcgg cggtgacacc gagcgctaca
     7021 ttgcgtgtct caagcaggcg tttcacgagc tgccgctgca cagcgaagcg tttcgcaagc
     7081 tgtcggatta ttacaaatct cgtcgagact tcatcggtct ggcggtgatc caagaaaacc
     7141 gcatggcggc gcagtttgag acggcgcggc tcgacctgca aagctatgcc aaacagctcg
     7201 aggagctggg cgagctgtat gaaaaaaagc tgctcgacgt ggtgtcggcg gcggcgatct
     7261 ggcgacgcat cgacgagctg atgccgtctt tgcgatcgca aagtgaacgc aagcggctgg
     7321 ggcaaaggct gtcccgcacc gagatgcaga tcaacgagct tgtcgtcgag cttgagcgca
     7381 tggatgtaga agatccagcg cgcgtcgagt atctgcgacg gctggcgcag ctttatcgcg
     7441 aaatccacga accgaagcat gcgacgacgg tgctagagca gcttctgcaa gttgcgcccg
     7501 gcgatctgcc gtcgatcaag gtgcttatcg agctggccga acagagcggc gacacgacgc
     7561 gacagctgga gcttttgcgc cagcaagcgg gcattgccgc cgacaaagcc gagcgactcg
     7621 gcattttgcg acggatgatg acgctgtgcg accagcggct tctgcgtcga gacgagcagt
     7681 tcgacggaca agacagcctg gaaatgatgg tctgggtctg ccgcagcctg ctcgcagagc
     7741 tgccgtcgga ccgtgacgcg ctcaagcgac tcgccgatgc actccagctc gtcggcagca
     7801 aaaacgagct gctcgaagtg atggagtcgt atctgaaagt cgcgccgacg ccacgcgaaa
     7861 aactgacgct gcaccgtcgg attgccaaaa ttggcgaaga ctgcggcgat ctggcgcgtg
     7921 cggtgtctca cctcgagcga gcggtgcgca tttgcccgcc cggtcccgag gccgaagaag
     7981 tgctgtgcga gctggctcgg gtctacggcg cgcaaggccg gatggaactg gcggtgcaga
     8041 cgctggaact ggtgcaaaag cagagcagcc gatcggggcc agagctgcat cggctgctcg
     8101 ggcgactgac gctgcaagcc gacgatccgc agcttttgga aaaaagcctg cgggcgttcc
     8161 gcgaagtgct ggtgcggctt ccgacggaca ccgaagcgct cgcggcactg caaaaaatcc
     8221 atcgcgcccg cggcgagtgg aaagaactcg aacaggtgct gcgcaagcag cttgagccgt
     8281 ccgatcccga gctgccgccg cgtgagcgtc tgtcgctggc gctcgatctg gcggaagtca
     8341 tcggccaaca cctcggcgat ccgcgcgcag cggcagagct gctggaaaaa gtgcaggccg
     8401 aaacgccgat tgtcgatctg cgcgtccacc gtcacctgcg tggcctgtac gaagagctcg
     8461 gcgacgtgcg acgggcggtg gcgtgcgaag aacgcgagct tttgctgacc gaagatccgg
     8521 tggcccgcgt cgagcgcgcg atcgaaattg cgcgactttg gcagacccgc agcaaagatc
     8581 acgcccgtgc ctatctgtcc tacgaacgcg cagtgcggct gatgccggac ctgccggaag
     8641 gaacgccaga aagggccgca gttcgtcgtc tgattacgca agcgcttgaa gcgatgagcc
     8701 agatggcagt tcatcgcgga aaatgggaag atgtcgtctc aattggccaa aaacggctga
     8761 atttggctgt tgaggaaggc gaagcaatgc aagccgccgc catcttggtg gagcttgcgc
     8821 aggtgtacga agaaaagctg agtcagccca ccgacggctt cgcgatgcgc atccaagcgt
     8881 tcgagctgtc gccagacctt atgtcgatcg ccgagctggc cgacctggcc ggaaagtacg
     8941 ggcagtggaa gccgctcgcc gatctgcaca tccagcgcgt cgaaagagcg ctcagcgaaa
     9001 aacacgacgt accgcttgat tcactgctcg cagcgtcgca gattttggaa caaaagctgg
     9061 gcgatccgat ccaggcgttc cggctggcga aaaaagcgct gccgattacc gtcagttcgg
     9121 cgatggcggc gaccaatccg cgcagcctgc aagcgcgcct tttgtctgaa atggaccggg
     9181 tgctgcgggc gctgcacaaa aaaggcggcg agcagagcag cgagctggct ttggagtcgg
     9241 tgtcgctcgc gcgggagctg gccggactgt accgtgcgct cgtcgacgag ctggttcgat
     9301 cgacggatgc gggaatcgaa gaaacagcgc tgcgcatcca cgttctgctc ggggaatcgg
     9361 ccaaagttcg cgagcaagtg ctgtttgatc cagccggtgc gctgggcgat cggatgcacg
     9421 catttaccgt cggtggagaa cgcgatcaca gcgaagatcc acaggccgac gaagtattcc
     9481 agttcaccgt cgcggaagta catcgtctgg cgctggtcag cggccagatc aaagaagcgg
     9541 tcgccgtcga catgcgtcgg caagagcgtg ctcagacgga tctgcgtcgg cagcaagtgg
     9601 cgtgtgaaag cgctggctgg ctcgacgatt ttggcaacga tccgcagcgg gcgctcaaag
     9661 cgtgcatcaa agcgctgccg atgtgtcagg aaggcagcga cgcgcaggcc gaaattcgcg
     9721 gtcgtctgca ccggctcggg cagaagatgg gtcttttggc gtgggacgaa gttgcacggg
     9781 ccgagcgggc gctggcgcaa aacgatccga aaaaactgcg gcagcgactg ctgtacctgg
     9841 cggcgctgtg gcagcaagga gcgcaggact cggtgcgcgc catcgacgcg gcgggccaag
     9901 cgctgcggct gacgtttttc ccatcgggtg ttccgacggg caagtcggcg cagccgttgt
     9961 cggaactgct ggtgccgcag ctttccgccg agcttgtctc ggagcagcag cagattcgcg
    10021 gcgtgctcga cagtctgttc cgggcagcgc aaggtgaaag cgagccagcg gcgaagctgg
    10081 tgacgctgct cgacaacctg gccaatcagc tgaccgaagc gggcgcaccg cagtgggcgg
    10141 cacgggtgat gctcgacgcg gcgcaagcag acgaaaagcg cggtcggttc ggtcaagccg
    10201 aacggcgcta ccaagaagcg agcaagcaga gcgaagtcca ggacgaagcc atgagcggtc
    10261 tggagcgcat ctatcggcag cagcgacggc tggccgatct ggcggcgctg ctcgaaaaac
    10321 gccgtccgct gctcgcggaa acggcgcagc tcaagcttct ggtcgaactg gccgaggtct
    10381 atcgcgaaat caacaagcac cagcaggcgc tcaacgcgct tcagcaagcg gtggcgattg
    10441 atgacagcga cgccggaccg cacctgtcga tggccaagct gttcgaaacg caaaagacgt
    10501 ttacccgcgc gattgaatcg tacaaagcgg cagcgcagcg cacaccgtcg gcaattgatg
    10561 gcgccaaggc acttttgcaa gcggcggatc tgctcgagcg caagctcaac gaaaatgacg
    10621 aagcgctggc gctcaccaag caagcgctga gacggacgct cgtcgagcaa ggcaagcgac
    10681 tttcgcaagg cgagcttttg ttgccaggcg atccactgcg cgagccgcgg gatgcagcgt
    10741 ggaatcacgt cgagcgactg ctcgggaagc tgcagcggca agaagaaatg gacgcgcttg
    10801 tcgacgagcg gttggaggtg acgcctgcgc tgctccacga cgagcgagaa agcctgctgt
    10861 cgaaacagct acagacgctg aaggcgaagc tgcaaacccc aagcgaagtg acaccgcgcg
    10921 atggcgaaga agcggcgacg ccaagtcccg atctggaatc gcgccggctc catctgttgg
    10981 cggaacttgg caagctgcga ccgacggacg atacgctgct cgacgaacag caagccatcc
    11041 tgacggcgcg caaagaactg gccaaagccc gcgagatcgc cgctcagcgg ctccagcttg
    11101 cggaaaaaca agccgccgat ccgtcgacgg tgagcgaacg gcaactggcg gtggccaacc
    11161 tggacatcga gcttggccag ttcgccgacg cagaaagtgc gctcgaacag gtgcttttgc
    11221 ggtcgcccac atcggccagc gcactgacga cgctcgtcga gctgcacacg cgcacgggca
    11281 atcacggcgg tctggtcgcg gcactggtgc gaatgtcgtc gcagcagatc gatctggtcg
    11341 cagcggtggg cgcgcttcgg caggccgcgc aagttgcact gacggcgcag gacgatgtgc
    11401 cggcggccaa aaagctgctc ggtgatgcgc ttgtgcgtat cgatgcagcc ccgacggtga
    11461 caccagcgca aaaagagcag gtgcgtcgcg cgcttgggca agtgctggtg ccgctgttcg
    11521 acttggcccg tcgggaaggc aacacctcct tcgcgcaaga actggcgcag cgagcgattc
    11581 acgaggcgga tctcacgggg acgcacgccg cggacatgca agttcacctc ggacaagaag
    11641 cgctttccgc cggcaaatcc gacgatgcga ttcgcttttt cgacgcggcg ctttcggctt
    11701 ctccgggtct gctcgaaccg acgctggcgc ttttggaact tctgggtccg tcgggaaacc
    11761 acgcgcgaat cgacgatctg atcgaagcgg cgctgaaagc cacgtcgacg ggggaaagca
    11821 ttctgcccga tgcagagcgg gcgcggcttc tgcgcaagca agcaacggca cggcaagagc
    11881 tgggacggct cgacggaacg ctggacgcgc tgaaggcggc agagctgatc gttcccggct
    11941 cgctcaacga ccaaatctgg ctcggtgaaa ccgcgtttgc gcagggtgat ttcgagacgg
    12001 cacattcgca gcttgccaag ctcgtccgct tcgttgcgga aaaagacgcg ctgccggcgc
    12061 cgctttcggt cggacagctt gcggatctgc tggatcacgc cggtcagtcg gcagccgaac
    12121 tgcacaaagc cgaggaagcc cgctcgctgt ggcaagcggc gctgcaagtc gtcccagatc
    12181 attcgtcggt gatgaaccac tatctggaac ttctgctgac cgcagggcag agccaagacg
    12241 ccgagcaggc tctgtcgatg ctgacgacgc gagcaagcca agcggcggcg gcgggagaca
    12301 cagccggcgc gcgacgagat atccgacggg cagcggcgct ttcggctgga ccactcggcg
    12361 acaacttgaa agcgcaggag tttttgcggc aagtgctgac gatgctgccg cctctcgacg
    12421 attcaagcga tgcagcgctg cgcgacgagt ggacgacgct gctttcgcag gtttacgaca
    12481 acgccgaaaa gctcggtgag cttgcggaag cacgcgggta tgcggaacga cttgcccagc
    12541 tttccgagac ggtgaaaggc caaagtgtgt ggctgcaccg agcggcgctg tgtgcgctgc
    12601 gttgtggcga ttcggcgggc gccaagaccc tggtgctttc ggcgcttgcg gctacaccgg
    12661 cgcagctcgg gctcatcggc gattatctgg aacaagccag cgacgacgaa gccaacgaaa
    12721 agttgccggt tttgctcgat gcggcagccg cgacggaaaa cagcgcgacg acaccgaagg
    12781 gccccgattg gcgagaccag ctcaaaacac tgtgggtgcg cgccgcggaa atccacgttc
    12841 gcaagggcga tggtcaaaaa gcggcccagg cctacgagcg ggcgctttcg acggtggctg
    12901 atctttccgg caacgaaaca ggcacgctgc gacgggctct gctcgaagta ttgccagata
    12961 cggaacatga ccgggcgcgg acccatctgc atgcactgct caagagcgcg ccgcgtgatg
    13021 tgtcgctgct gagcaaactg cagtccgtcg aagaacgcgc ccaaaatccc ggtgcggcgt
    13081 ggcgtctggc gcagcttctg cacctgctgg acgcaaattt gccggcgccg ccgctgcctt
    13141 cgctgccgcc caaagacatc aagttcgacg aagccgatca ccagcggctg gccgaccccg
    13201 aagcccgcgc gatggccgac gtgctgtcgt cgctgtggga tggcgttttt tcgctcaaag
    13261 caccgacgct ggatgcgctg ggggttcagg gcaccgatcg actccaaggc aacgaaaatt
    13321 cccccgacga gctggctcgg gcctttgcgc tgtgcagccg ggtgctcggc aaccagcgcg
    13381 gcggtctgta ccggcctgcg ggtcatgcgc tggccctgcc ccgtctgatg gcgaagctac
    13441 ccacagcggt catcgcgtcg ccgcagatcg gcaaacggcc accgggcgag ctggtctttt
    13501 tgctcgcgcg tgccgtcgag agcttgcggc ccgaatacat catgccggtg gcgatgccag
    13561 cgacggactt gaatcggctg ctccagcttg cggtcaaggc gttcagtccg cgccagaccg
    13621 ccaagcccac cgacgatgtg acgacgtgga aacgagagct ggcctaccgc gcgagcaaaa
    13681 agctgcaaga cctgctaaaa gaacagcccg atctggatcc agcagcgacg ggatatcggc
    13741 tggcgatccg ccgcgctatc ggtcgtgccg cgctgctcgt ttcgggtgac ttgctggcgg
    13801 cgcgcaatgt gctcttgcag atcgatgtcc ctgccgcgca agacgctggt tcgacggcgg
    13861 ctggctacta cctgcggctc ggcgaaggcg aaagctcggc gctggcggac gcggaatccg
    13921 atctggccga tctttgcgct ttctttatcg caccacagaa cgccgcgctg ttcgacaagc
    13981 tccaccctcg gtaagatcac gctggttgcg accggtttcc cgtgaaccag cgcccggtgt
    14041 atgctcggcg cgaactcaaa gcacgggtga cctttgaaca cgaaaccatc tctcgtcagg
    14101 cccgccggct tctcgtcctt tttgcttggt ctgctcgtca gcgtcagcct gcttggtggc
    14161 tgtcgcgagc cgtttgacgg atttgctcca cgcaccgacg ggagcagcga tctttcaacg
    14221 atcagcgatg cctcggtccg cgacggtgcg acggtctatg acctcacgtc gaaggccgat
    14281 ctggcctggc ctgcgccgag cttgccgccg tcgagctgct accagcggtc agcgggtgtc
    14341 accggcatcc aagtcgtcct gcgcgtcgac cagtacatcg gcgttcgcac aggacgcaac
    14401 ggcccccacg aaatcgtcca cggcacgatc atcaacacgc cgtgggttta ccaagcgtcg
    14461 atcgtcgaca ccaccaacgt cgaagtcgcg atgaatatcc aaacggcgac cgatccgacg
    14521 ggcctgccga tggaaatccc ggtgcgcgcc cgcgaccagc tcgagcttga aggcgaatac
    14581 attcccaaat cgaccgcgaa tgcgacgacg gcgctcggtc cctccgctgt catccacttc
    14641 acccacagtc cttgcggcta cgccgtcctc ggtggcacca agtacaagta accgcccgcg
    14701 gctacggctt catctttggc tgaagccacg ccgaaatcgc catcagttca tcttgcgtcg
    14761 gcgtgcccgc acctttgggc ggcatgggcg ttggttttcc gtcgatcacc cgctgcttga
    14821 tcgccgcgcg gcgaatgtcc cacgacttgt acgtcgacag atccaggttc gcgctgcccc
    14881 ccggcatgtg acacagcgcg cacagccgcg taaacaccgg ctgcaccgtc tgcctccaca
    14941 cctcttcatc cgcaccacct ccggaatctt cgccgaagcg acgcagcatc ccagacgaaa
    15001 gcacccagac atcccccgtc ggtgaaccga acagtttggc atccgcaggc agcgcgagtg
    15061 gcggcgactt ttggatcgca tcgctggcca aaagcgccag ggcatcgccc acctgtaccc
    15121 acacctcgcc gctcgcgagc gccagaccca aaagcggcgc gtccgccgac acataaagca
    15181 catcgagcgc cgagcccagc tcgcgactct gctcgcgata cagcgtgtct ttggtcgcaa
    15241 tcaccacccg gcccgccgta tcgatcaccg tcgccaccgc ggcttgtggc aggccaaacc
    15301 gtttgagctt gccgctgctc ggctcgaacg caatcgcggt gccgtcggaa atgcccacca
    15361 ctcgtccgcc gccgccttgc aggttccgca gcttgactgg atagcgaacg acgttttttc
    15421 cgtcggccac cgcgagctca ttttcgagcg caaacaccgt cgctgttgcc acgtcggcag
    15481 ccgccagatc agaaatgacg tcgctgcgca ggccatagcg atccgacaca tcttccagca
    15541 cggcgcgatc acgcagcctt cgcaggtgcc ctttgccatc gacgcccacg agccactgcc
    15601 cagccaaatc gcccgctgga atcaccgacg cagcgttcca gcttttgctt gtcgcatcgg
    15661 tattcagaag cagacccgca gtaaaaacca gcgcgccttg gtccgaaaac acgacggtgt
    15721 cttcgttcag ttccgcgacg gcagaaatct tcccggtcgc gacatttttg gcattccacg
    15781 acacactgtg gaccgcaagc ggcacctcgt ttagcagcgt cgaggtcggc tcgggcgact
    15841 ggcacccaag cccaagcagc acagccgata gaaacagacg acttggcagt ggcatgacgt
    15901 cgctctactt ggcgtagatg tccatgatgt ccgtcagcag ctttttgccc tgcgcaaacg
    15961 gacgctggaa ggcgttgcga ccgatgatcg atccatagcc accgccgtcg cgaatcgcgc
    16021 ggatgtcgtc gagcaccgca tcatcgttgt ctttggtttc gccacccgaa aagatgatga
    16081 cgcgcttgcc gtcgaagcag cacttgacga cgtgacggac gcgctcggcc atcgtcgcgc
    16141 tcgccggttt gtgggcaccg tagaccttgg cggcctcggg ctgttcgatg cgatctttgg
    16201 gcagcttgac cttgacgatg tgggcaccaa gctgcgccgc gatgtgcgcc gcatagcccg
    16261 ccacatcgag cgacgtttcg ccgtctttcg acaggttggc accgcgcgga tagctccagg
    16321 tcacgacgat caggccggct tctttggctt ccgccgtcag atcgcgaagc tgctgataca
    16381 tttcattgcg cgacgacgac cccggataga tcgtaaagcc gatgccgacg cagccaagcc
    16441 gcagcgcgtc tttcaccgtg ccggtcagcg ccgaacaggg ctccagcttc gacagcgaat
    16501 cactgttgtt tagcttgaga atgagcggaa tttcccccgc aaacttgccg gcacctgctt
    16561 ccagaaagcc gagcggcgct gcataggccg cacagcctga ctcgatcgcc agacggaagt
    16621 gatagtccgg atcgagcgca tcaggattca tgccaaagct gcggtacgga ccgtgttcga
    16681 atccctggtc gacgggcaaa atcaccatgc gcccagtccc agccagacga ccatgcatga
    16741 gaagccgcgc caaattgctc agcgtgccag ggttgtccga cggataatac gaaaggatct
    16801 ctttgacgcg cggggtgaca gacagggtca tggggactcc ttgacttgga aagttgcgat
    16861 cggaaagtcg cgggcgtggc aaacaatcaa agcgccgacg cggcccgagc atgccaaagc
    16921 cgccccggtc ctgacaaggc tcgccaaaag gaggttgcct aaagcggctc ggctgtggta
    16981 atcagcggcg cgcaagtgcg aaggaggaag cgatcagcga gagcaaaagc aacccgcaga
    17041 gcatgtggct tcaggcagcg aacctgagct acctcggtat cttttttggc gtttcaatcg
    17101 tcatcggtgc cgcgctcggc agctttctcg acaaaaaatg gggcacccat ccctacctct
    17161 tgatggtggg cgtggtcctc ggcatcgcta ccggcttcaa cgaactttgg cgtgtcgcca
    17221 aacgctacca aaagcagcta aagcaaaaat cgtgaacgac caaacccaaa ccccagcgcc
    17281 cccggtgaac actccagtac gtgctcactt tcgtgccgtc gagcgaatta cgctcgtgct
    17341 gggtgcgctg ttggtcgtgg cgtcgatgct tttggtatcg ctaaagcttc agctctcggt
    17401 gtcgtatggc gtgctgctca gcatcgtaaa tgcgcgggca gttcgttttt ttggggaacg
    17461 gctggctgcc cggcaatcgg tggggctggt cgtcgtcttg tttcagctca agctggctgt
    17521 gctcggtgtg ctgatctatt ccggtctgaa gctgttttcg ctcgacggaa tcggtctgtg
    17581 cgttggtctg tcgcttttgc cgctgtcgat cgtggtgcgg gccgtgcagt ggggaatgtc
    17641 cgcacccgct tcaaatggag aactggtgag caatgggtga ggaatccacc tggttcgatt
    17701 ttctgcctgg gctggccaat ctaatcgcct gggcgaagca ataccttggg cgcgaagatc
    17761 cgtcgcaggc cttctttttt cagggcggac cgttcgtcga ctcgcacttc aacctgacgc
    17821 acgtcttttc ggcgcttttg gtgctgtgct ttgtgacgct tggcgcgatg agctttgcct
    17881 cggcgatgcg caaaggtggc gacgacgcga ttgttccgcc gtcgcagttt tcgctgcgca
    17941 acctgttcga ggtcgtcggc gacgcgctgt ggaacctgac ccgcaacatc atgggcgaag
    18001 aaaacggtcg caagtacctg ccgctgattg gcacgctcgc ttgcttcatt ttctttagca
    18061 acgtgctgtc gctgattccg ggtatggtgc cgccgacttc gacgctgaaa acgaacgtcg
    18121 cgctcgcgct gaccgtgttt gtgatcaccc acgtcgaagg catccgcgcg caaggcatcg
    18181 tttcgtacct gaagcacttc gccggcccga tctggtggct cgcgtggatc atgattccga
    18241 ttgaaatcat cggtcacctg gcccgtccgc tgtcgctgtc gatgcgtctg atgggcaaca
    18301 tggtgtcgga ccacaaggtc gtcggcgtgt tcttcttcct ggttccgctg ctcgtcccgg
    18361 tgccgttcct catcctcggc acgatggtgg cggtgattca gaccctggtg ttctgccttt
    18421 tgtcgatggt gtacatcaac atggccgtcg cgacgcacga ccacgatcac gacgaacacg
    18481 cgggtcatga cggccacggc gagcacgctc acgcgcacta aacatcgccc gacgctttcg
    18541 cctcgggttg gctgatcaga cgggcaaaca gcccgtgccg ccgcaaggac ttcgatcagc
    18601 cgcaaattga gaagattgat tcacaaactc aagaccggta aagaaaccaa aacgcttcac
    18661 ggaggaactc gaaccatgac caccaagatc tccaagtaca tgctcgcggc gatggctgcg
    18721 ctcgtgaccc tgatggctac ctcggctgct tacgcgcagg cggcctcgcc ggaagttgcc
    18781 aaggcggaaa acgctcgctg gtacgcgctg gctgcggccc tcggcctcgg cattgcggca
    18841 ttcggtggcg cgctcggcca gggcaaggcg gcggcggcgg ccctcgacgg catcgcccgc
    18901 aaccccggcg catcggacaa gctgttcacc ccgatgattc tcggcctcgc cctcatcgag
    18961 tcgctggtca tctactcgct gctgatcgcg ttcctcctct acggcaaggt cagctaagca
    19021 gcccgtttcg tctgcggcca gcgctacagc gcctggccgt gacgccccgc ccccgacgca
    19081 aacgtcgtcg ccccaccgac tgtttctccc gacaaaatcg tctgcatccc aagctgaaac
    19141 tcgcgctgta acgcctcgtg cacaggcagc ggatcgtcat actgaagcag cgtcgacatt
    19201 cgatcactgc gcagacagct ttgcggcagc cgcgccagct cctgcgccag cttttccgca
    19261 ttttgccgcg ccgtgcccgc cgcgacgacc cgattgacga gccccatcga cagcgcttcc
    19321 gtcgccgata ccggtcgccc ggtcagaatc aaatcgagtg cccgtcccag cccaatgatc
    19381 tgcggcaaac gaatcgtccc tccgtcgatg agcggcacac caaagcgccg acaaaacaca
    19441 cccagcaccg cggtgtcatc cgcgacacgc agatcacaga aacaggccag ttccagtccg
    19501 ccagcgacgg catagcccga aatggctgca atcgtcggtt tgccaagccg cagccgcgtc
    19561 ggacccatcg gcccatcccc gtcgacggta agctgattcg gactgccccc tgcgaccgcc
    19621 ttgagatccg ccccagcgca gaaatgaccg ccttcaccat agagcaccgc gacacgcgct
    19681 tcaccgtcgc tgtcgaagtc gcgaaatgcc tgcgtaagcg catgggccgt ttcacgatca
    19741 acggcattgc gcacacgggg ccggtccaca atcaccgtcc atactgccgc ctgcttttcg
    19801 attttcacca tcgcggactc cttggctcgc tgttttgtcg acgggacgaa aaaatgtgaa
    19861 gctaaactcg caacttcttc gatacccagc cgaacacctg gacctaccca ccaaaggagg
    19921 ctcagatgtt cgccaaaggc tcaatttttt cgttcgcact gtgcacgctg ctttcgataa
    19981 gcgtcccggt ttctgcggcg ccaccaccgt cgcctgaagc gaaagcgatt gcccgcgccg
    20041 ccgtcgaagc agccgaactt agcccacaga aaactcgatc gctgcgcttg cgcctgcacc
    20101 tctcgtcgct cttgccgcag ctacgggtga catttggccg cggctggcag ctttcgtcga
    20161 gccgtgacta tctgctcgaa acaccagcga cggaaaatga ccgcataaac tacgcgatca
    20221 ccgcgagttg ggatctttcg cggctcgtcg tcccgcacga agaactgcac cttatcaaag
    20281 aagaacagcg acgggcgacg ctgcggctgc gactggaaga acggctggcc aggctgctgg
    20341 cggctcgctg tcgactccag aaaccggaac atccgaccga tcggaccaag ctcgacgagc
    20401 tggaagccac gctgatcgtg ctcaccggtg gcaagacgct tcccgctgtc gatgaaaaac
    20461 aagactgtcc agcggcgccc ctacgaatcg gactgacggc ctcagcaaat acgccagctt
    20521 ctgccgacga cgttctggca gcacccgcgc atgacggaac tgctgcccat gaaagcgccg
    20581 aaccgagcga gccagccagc tctcccgacg aataacaggc cagttgtggt atttcttcgt
    20641 ctcgatgatt gttggcgttg ggctcgacct gtgccttgtc tcgcggatgg aaaaagcgat
    20701 cacccgaagt ggcggtcgct ttgccgcaag agtattcacc agcgacgagc gtcgttattg
    20761 cgaaagccgc gcccagccgg cgcaacactt tgccgcgcgc tttgccgcca aggaagccat
    20821 cctcaaagcg ctcggcgtgc ccgaaggact gtcgtggcac gaactggaaa ttgcccagcg
    20881 cccgtcggga ggtccgcagg tcgttctgca tggggttgcc aggcaagccg cgctcgcgct
    20941 cgggatttcg gtcctgcacc tgtcgctgac ccaccaaggc gatgcggcgt gcgcagtggt
    21001 cattgccgaa cgatgggccg aagccagcga gaggagtctg taatgatacc gcttgtgacg
    21061 gcgacgcaga tgcgtgcgct cgacgaagaa accatcgccc agcttggggt cacaggcggt
    21121 gtgctgatgg aaaccgccgg acgcggtgtc atcagcgttc tttttgacct gcatcgccaa
    21181 ggccgcttcg acctccgcac cgcccaggtt tgcgtcgtcg caggtcccgg caacaacggc
    21241 ggcgatggca tggtgattgc gcgctacctg cacagccacg gcattgcctg cgatctgtgg
    21301 attgtcgctt tgcgaccgcg cattcggggc gatgcgctcg tacacctcgc cgccgccgaa
    21361 gccgtcggtg tcacctccca cttctgtgag gcagagtccc cgcttttgca acagcgcttg
    21421 gccgcgcttt cacccggcga tgtccttgtc gatgcgctgc tcggtacggg ggtttcgcgc
    21481 actgtcggtg gcggcctttg caccgtgatc acgcagatca atgccagtcg agccatcacg
    21541 gtgtccgtcg atctgccgtc cggtctcgac gccgaccgcg gcattccgat cgatgcgggt
    21601 ccggccccgg tcatcgtgcg cgccgattac accgtcacgt tcggcttccc caagctgggt
    21661 ctgtgcggat cgccgggctt tgtcttcgcc ggggaaacct tcgtcgctga cattggcatc
    21721 ccggctgctc gctgcgccca tcatggggtt actgcccagc ttgtgtctgc ccaggtgatg
    21781 acgccgcttc agtccgtccg cccaccgctt ggccacaagg gaagtcacgg acacctgttg
    21841 tgcgtcgcgg gttcggaagg caagctcggc gcggcgctgc ttgcgacgga agcggcgctg
    21901 cggaccggcg tggggctgtg cacgctggcg gctgcacgcg cggcgctcga tggttcgctg
    21961 gggagccgct gtcccgaggc gatgacgctg ccctatgatc cgcaggacaa acacgcgctt
    22021 gatcagattc tgtccgcact ccccggcaag actgcagtgg cggctggccc cggacttttg
    22081 ccgacccaag cgacccagga actgatcatc cgtctgcttg cagccgatac gctgccgatg
    22141 gtgctggacg cagaagcgct gaatcaactg gtcggcacgc ttgatgtggt caggcagcgc
    22201 agccagcgcg gtggtgagac agtgctgacg ccgcatccgg gggaagcggc gcgactcctt
    22261 ggcaccacgt cggcggcagt gcaggccgat cggacctgcg ccgtccgcgc gctggctgac
    22321 aagacccatg ctgtggtgct gctcaaaggc gccaagacgc tgattgcgcg gtctcacgct
    22381 tcagacacga cgggggtcac actggcgatg attccgacgg gaaatgcggc gatgggatcg
    22441 ggcgggatgg gcgatgtcct gaccggcatg atcggcgctt tgctcgcgtc gggaatgcat
    22501 gcttttgacg cagcgtgtct tgcggcgtac tggcatggtc tcgctgggga tgtggcggcc
    22561 acacgcaaag ccccgggcag catcgtgctg gccaccgaag tcatgcagga tttggatgaa
    22621 gcgaggaaag taggcgggac gtttgcgagc agccagccgt cttggccggt gtggccgctc
    22681 gcgtgggcgg cgagtcgaac ttagccgcgc ttgcgctcga actcgaacgg gttctggcgt
    22741 tccgccgggt aatcgcgacg atcctggccg gtgtaaatct gacgcggacg ggcaattttc
    22801 tgctcggaat cgtcgagcat ttcgcaccac tgcgccagcc agcccgaggt ccgcggaatc
    22861 gcgaacagca ccgggaacat atccaccggg aagcccatgg cctggtagat aaggcccgag
    22921 taaaagtcga cgttcgggta cagcttgcgc ttgacgaagt agtcgtcctg aagggcaatc
    22981 cgctccagct cgagcgcgat gtcgagcagc gggttgcggc ccgtgacttc gaacacttcc
    23041 tcggcgagcc gcttgatgac cttggcgcgc gggtcgtagt tcttgtagac gcggtggccg
    23101 aagcccatca gcttcttttc gcccttgcct tctttgactt ccttgacgaa ggccgggacg
    23161 ttggcaaggc tgccgatttc ggcgagcatg cgcagcaccg cctcgttggc gccaccgtgc
    23221 agcggaccgt acagcgccgc cgatgccgca gcgactgccg aatacggatc gacctgcgag
    23281 ctacccgtcg agcgcatgac cgaagtcgag cagttctgct cgtggtccgc gtgaaggatg
    23341 aacagcacgt cgagcgcgcg ctcgagcacc gggttcggct tgtagcgcga ctcgctcatc
    23401 cgcttcatca tggcgaggaa gttgccggcg aagctcaggc cattgtcagg atagacgaac
    23461 gggaagccct ggctgtgacg gaacgaccac gccgcgatgg tcggcacctt ggcgagcagg
    23521 cgcacgacgt tgagcatgcg gatgttcttg tcgtagacgt gcttggcttc gggatagaag
    23581 gtcgacagcg cgccaatcgt ggcaaggaac atgcccatcg ggtgcgcgtc gtaccggaag
    23641 ccctcgatca ccgtcttcac attttcgtgc acgaaggtgt gggtggtgat ctggtcgatc
    23701 cacttgctaa gctgcgcttg gtccggaagc tcgccgtgga acagaaggta ggcgacctcg
    23761 aggtagctcg acttttcggc gagctgctca atcgggtagc cacggtaacg caggatgccc
    23821 ttgtcgccgt cgatgagggt gatggccgac cgacaggacg cggtgttcag gtacgccggg
    23881 tcgtaaccca taagaccgaa gtcttcactg tcagtcttga tttgacgcag gtcgacagtg
    23941 cgaatcgtac cgtcggtgat gggaacttcg taggtcttgc cagtgcggtt atcggtgatg
    24001 gtcagggtgt tcttcgacat ggtccctctt gtagccaaaa agggctgaaa cggatgatcg
    24061 agccgccaag cggcggcggg tcagactgcg tcaccttatg gctggtctga ccaaaaactt
    24121 tggcgcgcag tctagcggaa gggttttcgc tcgtcactat cttgcgccaa cttttgatgc
    24181 gaatcactgg cttttcgctg cgcgaaaaat cgcctctggg aacaacaagt cgcggcgagg
    24241 cttgcgaaag agaacgactt tggccatgtt tgcgcgaccg atgtccagcc tcgaatctga
    24301 cccgacttca cctgcgccag catttgcgac gagtccgccg cttcctaacg cagcgcgtta
    24361 cgcattcctt tcgcgccccg gtccagccct cgcgtgtctg cttctgtttt tgggcctttt
    24421 gcttgtgcca ggcctgacga gctttggcct gtgggacccg cacgaagtcc gtctcgtcga
    24481 aggcgccagc gaaccgattg ccgccgccga gctgtggaag cccgccaatg cgataaagcc
    24541 acggctgccg ctgcttccga tcaagctggg tctgtcgctg tttggcgtcg gagagctggg
    24601 cgcgcgactg ccgatgctgg cgatcggaat cgctgttgcc gcggcggtgc ttttgtattt
    24661 tgcgatccag ggtcgggtgc gcgccggtct gctgtcgggt gtggtgcttc tgacgacgcc
    24721 agcgctgttt ttgggggcgc gtcaggcgag cctgcatctt ctgccgatgc tgggacagct
    24781 tttggccgtg accggccttg cgatgctgtg ctggccacaa gcgggcaaaa cggtcgtgtc
    24841 cagcgtggtc ggtctgctgc tggcggcagc ggggcttggg ctgggcctgc tgacgtctgg
    24901 cagcctgatt ggcatggcgg tgccggcgct ggcggtgggt ctgacgctgg cgcttaccga
    24961 cggaccgatc ctgggggaaa tgctggcgac tgccgtggcg gggcttgcct tgaccatgcc
    25021 gttgcgacag atcctcgtgg tcagtcagct ccgcagctcg ctcgtcgtcg gacttggggt
    25081 gacgctgctt ttgcttggca tcgtgctcgc gctcttgtca cggcgatcga tctggctgtg
    25141 tgcatcgctt gtcggggtgg cgcttctgcc agcgctgccg cagagcctct ctggttattc
    25201 gggctggctc gccggggtgc cgcactggcc ggcgcatcga gacatgcagg ccgattcgct
    25261 ggtgaagacg ctcggctttg tgatgtttcc gtgggtggcg cttgtgccgg tggcgctggc
    25321 ccagcttttt ttggcggaca ccgaatcacc atcgtctgtg gaaagcgcga aatcgggtga
    25381 agcgctgcgc gcggcgtttc cgaacctgct gctcctgacg tggctgtcgg ttggctatct
    25441 tttgcacacg ctgcaggcgg cgctggtaca ggaacagagc tttgcggccc tgccgtctct
    25501 tgcacttttg gtcggtggct atctcgacgg gcagctccac aaccgacggt catcgatcgt
    25561 ggcggcggtc tgttcggggc ttttggcggt gtttgtcgcg cgcgactttt tctttggccc
    25621 cgagcagtat ctatcggccc agctcaccga gctgctcaag tggccggcgg tgccagccgg
    25681 aaccgatgtg ctggtcggcc aaggcggaca gatcgtaagc actttcggca tgttggttgc
    25741 gctggcgttt gcgatcggtc tgtatgtgcc gtctcgtcgg gcgattgtgc ttttgccagc
    25801 ggcggcgact gtgctggcgc tgtttgcgat gcacgggctg gttccggcgg tgacgcggca
    25861 tgtgtcgtat cgcgggattt acaccaaata cgaaaagctc ggcggcagag agctggggct
    25921 atacggcata caaaagtcgt cggcgcgcat ctacgggcaa aacacgatcg agattccctc
    25981 gctgcctgag ctgtttacct acctgaccaa agatgcgcag aagcgagcgt ttgcgatcgt
    26041 cggtgcgagc gagatggcgg cactcgatgt ccactcgttc caaaacaacc tgccgtatta
    26101 cgtcgtcgac gactcgaaca gccagtatgt gcttttgtcg aatcagctcg gaccggggga
    26161 aaaagaccta aacccgctgc gccgtctggt atcgacacag ccgccccagg ttcaggccga
    26221 actacacgcc acgttcgacg acaaaatcga gctgattggc tacgacatcc cgcaagaagc
    26281 gcagcgcggc accgagttca tcgtccgcct gcactaccgg gtcttggcgc cggtgctcgg
    26341 caactaccgg gtgtttttgc acttcgatgg atcaagcgca cgcatcaacg gcgatcacat
    26401 cccgctcggt ggcaaatacc cgaccaacta ctggacccgc ggccagtaca tcaccgacga
    26461 gtaccgcatg agcacctcgc gtctaaacca gagcgccggg tattatacgg tgtttactgg
    26521 cttctggccg ggcggggatg gagcacggat gaaggtgacg caaggcccgc acgagccgga
    26581 caaccgcgtc cgcctctcgg cggtaagggt caagtgatgg atctcaagtc gctggcagat
    26641 gtcgagcttc gagacgctac gtttccactg agccgatccg cgccgtcgaa ctttttgccg
    26701 gcgctgcgca ccccggtgat cctgcgtgag ctggtggcgc tcggggtgct cgatccgggg
    26761 ctgtcgaaag tcattgtcca aaacggtcgg gtggtgggct gctgcctggt cgaagtcggc
    26821 gcccaagggg cgtacatttc gggcctggga accgacgccc tatcgcaaca gcgaggcgga
    26881 tcgcgggcgc tgatcgaagc ggtgatcgcg gcgggaaaac aggcgcagct gggccggctg
    26941 tcggtcgaag tcagcgaggc cgatgccggg ccgcagggac tttggcaagc ggtgggcttt
    27001 gtgcctttgc ggacgcggct gcggatggcg cttttgggga cgccaaaccg cgcgcttctg
    27061 cccgacccgg acgatagagc gtgcatggcg gccacgcgca tttccgctgt gccttgtggt
    27121 gatgcgctcg ggtttttgca acaccacgga ccttcgcagc tgggcgcggc ggtttcgccg
    27181 gcagtgctgg gcaagctgga aaagcgcctg tccgctgcgg cgatgcttgt cggatcggcg
    27241 attgaagcgg tggtgatcgt cgacaaagaa cgcaagctgc tggtggcact ggcaggcgat
    27301 ccagcgtacc ttccgcagct tgcggtctat gcggcggcgc gactttcggc ggcgtatgta
    27361 gactcgctgg cggacgatga tccggcggtg gaggccctgg aaagcgccgg tttttcacgg
    27421 attgccgtcc gcaccgagct gtcgctttcg cttccgaacg cttctgcgta aaaagagcat
    27481 gcgcatggac acgtttttgc gggggcgacc catcgcttcc tgcctggcac ttttgctttt
    27541 gttgctgttt ccactgggcg gtcgcgccga tggggatggc ggtgacagcg gcgaagggga
    27601 cacgccacgg cgggcgctgg agcggttttt gggcaacgtc gcgtcgggac gctacagcga
    27661 agcagccgac gacctggaac ttcacacaag tgagcagtcg gatcgaacga agctggccaa
    27721 gatgctgggg gcggtcatcg accggcggct gtcggcggac cccgagtggc tgagccgggt
    27781 atcggacgca ccaagcgggg accaaaaaga cgggatcgcc caggaccaag acgaagtggg
    27841 acggattccg gcgacgccga ttgccgagcc ggtgcggctg cgccgggtct ggatgcggga
    27901 tcgcagcgaa taccgctggc agttttcgca caagacggtg cagcgggtgc cgtcgtggta
    27961 cgggcggctg gatgacgtgt ggctgcgaga acacctgccg gacaagctct tgcgcaaagg
    28021 gcccaaaggc ttcgtggtgt gggaatggct ggcgctgccg gtggtgctga cggcaagcgg
    28081 ctttgtcgca tggcttctga cgctgctttt ggagctgtcg ctgcgacctc tggcgcggcg
    28141 gtcggcttcg cgctgggatg acctgttgtt ccggcggctg cgacggcccg tgcgactgct
    28201 tctggcggtg gtgttctggg ggctttcgat gccgtatgtg ctcatcacgg ggcaggcgga
    28261 gcggatcgtc cacaactttg cgcgggtggg ttttctgctc gggctgtttt tctgcgtgtg
    28321 gcgctgcgtc gatgtcgccg tacagatcgt gcgggaatcg gactggctca agtcgcaccc
    28381 ggcggccaaa ggggtcatcc cgctggggta tcggctggcg gaaatcacgg tggcggcgat
    28441 cgcggtggtg acgacgctgc aagagctggg atatccggtg acgagcctgg tggcaggtct
    28501 gggcatcggc ggcttggcgg tggctctggc ggcgcaaaaa acggtcgagc acgtctttgg
    28561 cggcgtgatg ctgagcgtcg atcagccgat gcgggtcggg gatctggtgc gggtcgacga
    28621 tgtggtgggg tacgtcgaac agattgggct gcgctcgacg gcgattcgaa cgctggagcg
    28681 gtcgctggtg aacttcccga acgggaagct ggcggacatg aaga
//
LOCUS       DAORGN010000045        28679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_625_length_28679_cov_6.219257, whole genome shotgun sequence.
ACCESSION   DAORGN010000045 DAORGN010000000
VERSION     DAORGN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28679
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_625_length_28679_cov_6.219257"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKL17_07295"
     CDS             complement(<1..364)
                     /locus_tag="PKL17_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK44569.1"
                     /translation="MASSHALVVEDEEQFRKVVGEFLQGAGYRVTLCESGDKAIQLAS
                     ELPFDVIISDVRLGYGPDGLDVLSAFQKHQPQAPVILMTAFGDVENAMKAIQQGAYDY
                     ISKFPFQPQELLQCVARAL"
     gene            complement(407..2509)
                     /locus_tag="PKL17_07300"
     CDS             complement(407..2509)
                     /locus_tag="PKL17_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK44570.1"
                     /translation="MRKLLPAIALLWTTAVSASASAQTAGPGFQVNRYEPTAAGEWSL
                     LVEHPWYSSTRYFAAGVTLNYAHNPLTYGLRFADGSFVRGGAVIGNQLIGHIDVAGSF
                     LDRVLLSLSMPLTMYESGESQYGVSPVSGAAVGDPRIGARVRLFGQPYQGPASLSLGV
                     DVWIPINTFASPPPFPAQTGESGVRALPKLIAGGLWKSLMWSLTAGFYWRPNASLSKL
                     QPVNSNSIGAEVQFGAAVAYANRSLGLAVGPEVLVSSVVAADSAGGSRAFKPDSMGIE
                     LLLGAHYSLAKTMQISLGGGTTFFQVPGAPDFRFLTRLAYAPFRDAKAPDADGDGIPD
                     HSDACPSEAGVASKDPKKHGCPLPNDRDRDGVIDPDDLCVDVPAGKNPDPERRGCPLG
                     DKDGDGVLDPQDLCPTEAAGKVPDPKRLGCPAGDADSDGVLDPEDLCPSEPVGKVPDP
                     NRKGCPDKDSDGDGVFDSQDQCKDVHAGLMPDPMKAGCPSPDRDGDSIADPVDACPDK
                     KGAPSADPKKNGCPGLVVVESGQIVILKQVFFATGKDTILKVSYPVLDAVVMALKAFP
                     DIKRVRVEGHTDNQGKADYNTSLSDRRAKSVMAYLIVKGIEARRLDSQGFGPSKPIAD
                     NKTVKGRALNRRVDFVIVEPNLPMAKAPLPPPPVQDEAKPKAPVGKAPVKAPVGKAPV
                     KPAAKAPVGKAPAKPAAK"
     gene            complement(2519..5110)
                     /locus_tag="PKL17_07305"
     CDS             complement(2519..5110)
                     /locus_tag="PKL17_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44571.1"
                     /translation="MSHPSSPAATIRRQAGLMFGLLGLWAGCAATDFAEVPTLQTANL
                     AANPDGDLTATMLGQVVNRYAPLSAAAAAGDTAVSVTNLAALNVAPDDLLLIIELQGA
                     QIRSTDDDQFGSVQSLQGAGLYELVKVRSVDLASSRVLLDTRCGGLRNAYSATGKSQV
                     VRVPQVGNLTIPSGTSVVAQPWDGQKGGIVALRASGNITIDGALDVSGQGFRGGSGQT
                     GLVVQTDESPDRAGYRTTQASLGAAKGESIAGFSADYAALGGAFGRGAPANGGGGGNG
                     VKSGGGGGANGGAALAYDGSGVMDANAVGKAAWNLDPAAMLAGKLTTSAGGGRGGYSG
                     SSSDQDALVVGPGTASWGGNQRRERGGRGGHPIVQTPGSRLFFGGGGGGGDASGKTAA
                     AGAPGGSGGGLAFLWGSRLSGAGRISASGLPGGSTSGPSHDQAGGGGGGGGTLALFVP
                     VLEGSLKLQAAGGLGGNQGAPAGTDAAGPGGGGGGGVLVLPGTLPVTVARSTAGAAAG
                     TTLAAAVSEFPVNGATRGSDGEEIVFVPGSALSGCMPTDLSIAVTSTQKSAVPGLSYT
                     ISVTVQNLGSETVIAAPLSSLLSPLGIPSVNWSCSAIPATVGDVASCNPNRGFQDLNS
                     TVTLSTGQKAVYQVVIAVPSALTGTLTYQATVAAASGQSDVDLSNNVASLSVPIQPVA
                     DLQALVTMAPQPAAPGQPISVLAQARNMGPSDARDIVLRFVIPDGFRVLREPSSATMT
                     CSGSAETGYECTAAQLARFGTDDVLLVLEPVDSPSALTFADSVRGSVGDDTPGNNQAT
                     ATVQYDSSLAPYRPAQLAGGGFGCGVTQAFPATKSSLLFGSAVLLFSTALRHVRRLRR
                     GRLFRNR"
     gene            complement(5209..6333)
                     /locus_tag="PKL17_07310"
     CDS             complement(5209..6333)
                     /locus_tag="PKL17_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK44572.1"
                     /translation="MMLSEKLSALELSRLAVWVYDNEHAKIVWANQEALLLWKAQSRA
                     ELYARSYKDNSAATQARLDSYMRKVSSGEEVAEDWTLYPRGKPTTMTLHGTGVSLDDG
                     RLGILFQAVVKDQSLDGSMVRGVEVLRHTSLLVSLLDENGDELFHNPTVLRTFAAETR
                     FQSWFPETWQTILRTVSSGDRYQAELPVQTAAGDRWHLVEAQAATDPLTGKRGVLLQQ
                     LDVSQRRQAEELAESRSRLVAELQSALQLVETQRQEILALSAPVIDVGRGILAVPIIG
                     ELHDVRGAELLHRLLEAVSAQRARFVIVDLTGCANLDDEGARLLLRVTRSIALLGSQA
                     IVTGVAAQLAKAMVQAGVDMTQLVTLRNLRDGLDYCRKLT"
     gene            6417..8813
                     /locus_tag="PKL17_07315"
     CDS             6417..8813
                     /locus_tag="PKL17_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNK44573.1"
                     /translation="MKTENDPCAPPIAAFGSDSAKSNFIRDIIDADLQSGRHNTVVTR
                     FPPEPNGYLHIGHAKSICLNFGIARDYKKAGHTAFCHLRFDDTNPTKEDIEYVESIQH
                     DVKWLGFDWGDKMFFASDYFEKLYDIAVYLIKKGKAYVDSSTLEEIREGRGSLTEPGK
                     DSPYRSRSVEESLDLFARMRKGEFSDGAHVLRAKIDMSAKNMLMRDPVIYRIRHAHHH
                     RTGDAWCIYPMYDYAHPLSDAIESITHSICTLEFENNREFYDWLVAEARDLLPSLPHQ
                     YEFARLSLAYTMMSKRNLLKLVEEKVVSGWDDPRMPTIAGLRRRGYTPEAIRDFADMI
                     GVAKANSQVDYEKLEYCIRNDLNYRAPRRMCVLDPLKVVISNFPEGQREEVAAADFPL
                     DVNKPGTRPLSFSREIYIERSDFEEHPPKGFHRLSPGQEVRLLFSYIIRCEDVIKDAS
                     GHVIELRCSYDPQSRSGQSSRRVKGNIHWVDKASAKPCEVRLYERLFVAQQPGVERDF
                     HEDLNPESLRVINTALIEPSVESDEAGSRYQFARQGFFASDVKDSRPGHLVFNRIVTL
                     RDSWAKSVSDGEAEEKPTEKAAAKPESAEKADDRSRPQKKSRAQIRAEARQKDPQLAA
                     RFARYRDDLKLGEEEADILSGEALVAEFFDSACAIYANPQSIAKWIMSELLRAQKDRP
                     LSELPLGPVQLAELAKLVDEGEISTSAGKTVFAALMSEGGSPKALVDKLGLHKVADQG
                     ALEPIVDKVLADNADNVARYKEGKKTLLGMFVGQVLKATGGSADPTLARKLIIARIGE
                     "
     gene            complement(9080..9517)
                     /locus_tag="PKL17_07320"
     CDS             complement(9080..9517)
                     /locus_tag="PKL17_07320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNK44574.1"
                     /translation="MSELVSSFDLQIEQIDGYEFRVKFDKPQHPELHMDEPAPLGKDT
                     APNPSRILAAAIGNCLSASLVFCMSRNGAKLSGFKSDVHVELTRNEQKRLRIGKVDVT
                     LRPQIDGDAAALEKCLATFEDFCVVTQSVRQGLPVHVKVEPQK"
     gene            complement(9570..10688)
                     /locus_tag="PKL17_07325"
     CDS             complement(9570..10688)
                     /locus_tag="PKL17_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNK44575.1"
                     /translation="MSQSADKTTPESPPVPPVFLVEMALRLRRFLLRAADAVVPPYVA
                     LLDRFFGAATTMLLHSAAELRIADLLAKDPLTAAEIAARCKTDPALTERLLRALVGIG
                     VFARSEDGKYRNNRVAQGLRTGVPGSVRGFVEFFGIPEIVDAWQDLPNVLRHGGVGFQ
                     RVHGRGVWEWLATSKVGADSFVEGMSSMTEEVSSSIAAAYPFDEVKKICDVGGGVGIV
                     LAAALRRHPHLSALLFDEEAMLEKAPEHLRKRGVLDRVELAPGSFFGSIPRGADAYIL
                     KTVLHNWNDEDACTILRNVRAAMEPGHRLLVADFVGGPDAVTTLIPFMDIAGMLIYSG
                     RERTEKEMALLFEATGFRFRRLIPLPAGQAIFEGIAVA"
     gene            complement(10779..14351)
                     /locus_tag="PKL17_07330"
     CDS             complement(10779..14351)
                     /locus_tag="PKL17_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44576.1"
                     /translation="MKRIWVFAFCLLSIVSLATCQRESESSLLRAFRVTDRNQLFGGG
                     PRALGEVGDYVLQNDRIRLVVQNAGFSRGFGVYGGGIIDADLRRFDEQGRNESNRQGG
                     NDIFAEMFPSFFFQAVACDKVEVITDGTKPYDERYGTNRVHYDEGTAVIRASGGGGEF
                     LTLLKVFDSLFLNYLLPTASHSSDGNSELVRLALLLGLPFADFAAELNKLQNENARYE
                     VDYVLRPSVRHVEIHSRIINKTKKPLQIPSSVLANSTFQQQIGGVDLSTVRVPIGMVM
                     LYGRLNNVWLPGTGFDLRHPLERSFGRGLPLPAFNGVVTEFIASASSRMNDRVSYGLI
                     AAPSETNFVKQNADAYRNLGKYKDTFTPIDNTSLLVPFTAISFIGVFSDSVKTTIAPG
                     GFVEMTQYFIVGGGDVASIVDDVLSLRKTQSGTYQGILRRQQGGDPTSHGQIVIYQEL
                     GPQSGVRSSSFAKDDDYLDAGLRLCQTAGKDTLCRPYSQDYPDDAGNLFGNLPPGRYA
                     YRVQSEGRPIGPFVPFEIKAGESTFIDPILPPATYIQAAVVDDSGRPVPAKVSVVGKY
                     SQDLTNAQRRSGAVFDLQAGEDNRFTDMLPDSKEGQRAYIEAVAYTGADGHVTIPVRP
                     GDYLVYFSRGFEYDLVSAPVSVALGNAAQATGTLTRVVDTSGWVSMDGHVHSENSIDS
                     RMALTPRVTSAAGEGLEVVISTDHNFVSDWRPQLAALGLTPWLATFVGIEFTTLESGH
                     FNSYPLDYKVGPVTHGAFEWFGRPPDELFAGLRQLADPAAQTNIIVCNHPRDSTQGYF
                     NQYGRSSVTGDMVAQGTSKRLAGPNGRAFFDEKGKSRLSYGCDALEILNGKLGHQIHS
                     VRVPTDWPKECYKPLPSGFDPKTMLDPCSANGKILRPSSTTDALVPGTILLDKTPSAD
                     PGSSGLDNVEAVFPGAVDDWFHMLNQGFRHTVVAASDSHADVGEEPGAPRTYLRVGHD
                     DPVRVDAAQLVDVIKNQRAVTLSHGPFLTFTVTVPTASGGQTEPVQIGSEVNAPDGKV
                     TVNYRLSAPPWVSVARLHVYVNGRLVQRIPIDPDRNLSDRAERGGPQSGQIPLQLSRD
                     SWIVLEAVGDRPMFPVITGTEEPFLLVSDAVGALAGPLGIAATTDIGAVVVGNPQPYA
                     LTNPIWVSTSRAAAWRAPGVVPWKEINVPAQDPGVGVLRTRNHD"
     gene            complement(14385..15761)
                     /locus_tag="PKL17_07335"
     CDS             complement(14385..15761)
                     /locus_tag="PKL17_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNK44577.1"
                     /translation="MLRSSPASLACALVLSSFPFAASAQSYGDHVSPPPVDAPPPALP
                     EPAVQPVPAQDGTSALPAIAPGPAPTLIPSVTMPMGAASIWRIDLGGYIHGAYRWIEQ
                     PQNYNLAGRNNGFQLEQARVSANVQYKSLLAARVSIEGASEDRLSQSFPGGQLTARLR
                     DAYITWAPFRFFRVSVGQMVTPWDLDSMRSDAELPFVSRAVPVEGVQPTEGYTTRGMG
                     SDRALGISIHSGHIPLAGNTSFRYALFAGNGNGQNQILSDNNIPAVRGRAEFSYWGTQ
                     GLPNDQVRPIYAVTDDLRKPVLHLGIAAQWNPRTVGNLPDLIRETDVGVAADVAASLF
                     GIELQAAVLYMQTSRDTLSAVPPLERFGFWAHLRYTLPRIPVEITPGYRIASYSPRAH
                     LSTSPTSDMDKQVDAALGLLYHTFGIFVRPTRTFPVHMSLNYTITTEQAPNILNNDRF
                     EADIVAAF"
     gene            complement(15938..16900)
                     /locus_tag="PKL17_07340"
     CDS             complement(15938..16900)
                     /locus_tag="PKL17_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44578.1"
                     /translation="MKRGLLCCVLWLVALAGLSGCVTVNQHSAVRLSQQAVQSTRRLS
                     ASLDELRSGLGAYVDSQTLSVPLLGTAPLSHDTLCQIESVQQSLRLRKALLVRLGRAY
                     EHYILLAQEGADRWSGEVFDNLLVDLDPSEFALAAPLEPGCPPLPSAAIPKPSDPLPA
                     QAPRKKLSPSQQLQRASERLRGLLGQVLELVESEKPLYLSMQKQQLRARASVAKVLYQ
                     RHGVLLPTELLGSVLSGLGLSYGELDWQPQKTRGLPEADQKNLRAAVVQLLSRRVEQQ
                     SALYAAQLDEQLLFLHALVLQHQRLEAGQPLDFRQLAQWLIPSR"
     gene            complement(16904..17392)
                     /locus_tag="PKL17_07345"
     CDS             complement(16904..17392)
                     /locus_tag="PKL17_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNK44579.1"
                     /translation="MIVGVCRITLQLEDCHSDKDKRSVLRRIKDRVHQKWNCAIAEVG
                     DTESLDSAQLGIVVVSNQRGYTQSQVQKILQFIDDLAFARITDDEQDFLDYGDGAVEN
                     ALGGDYEHWEPGEPPPPKAGPKLQAPKLLEPAEYPWDSPDPESPSQNPESHVSPRSKG
                     GR"
     gene            complement(17466..20732)
                     /gene="infB"
                     /locus_tag="PKL17_07350"
     CDS             complement(17466..20732)
                     /gene="infB"
                     /locus_tag="PKL17_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNK44580.1"
                     /translation="MNTESVGTAGSKVAIYELAKELGIAHKDLVEKVRALGIEAKNHM
                     SRLERDEVDRVRRAFDKERHDNTVQERVSETVIRRRAKDGSRLHPEAQHAVANPPPPP
                     VVETRPPPVVVQSEPPRRIVRVVSPAPETPVRPVADVKPTPPPVVTPPSPPAQVVSEK
                     PAAVKVEPVAVKAKPVDPTPAPVVATTPAAAPVAAPTVESTAKAGDPAKKPARAKAAS
                     EPLAPAKADVVAEKPVATETPAPVKVEAAPAAPTPVVAKPPVVEAKPAPVESKPVAAK
                     AVEAPPVAKVEAPAAPAAKVEAAKPAPVESKPAAAKPVVQVAPKPAVAEAAKSVATPA
                     AQAKSQGVVSRPAPAAPVVETAAKSAAPRHVEPAVAPPPAEPVRAASASAPSATSPSQ
                     GAPAKVFVTAQTRRSLEGLGPTGRVIDLSAFKPQPSAKPAAPSSESPRNFRSGGERTD
                     TPEMGRGPRQEVSGERLRGGQESRRAPVGPVQPARGKLPPGRKPGKTQITTAAEHKRV
                     VKMGETIVIAELARQMAQKATDVLKKIWELGMRNVMINSSIDFDTAQLVASEFEWRVE
                     STAFQEQSVVVETTDKPEDLKPRAPVITIMGHVDHGKTSLLDAIRNANVAAGEAGGIT
                     QHIGAYKVTSQAGDVVFLDTPGHEAFTEMRARGAQVTDIVVLVVAADDGIMPQTVEAL
                     NHAKDAKVPVIVAVNKIDKQGAQPDRIRQQLAAHGLTSEEWGGETIFCDVSARTKIGV
                     DRLLEMLALQSEVLELRANPNKAAKGAVIEARMDAKRGPVATVLVQEGTLRVGDTVVV
                     GEEMGKVRAMSDDKGRTLAQAGPSTPVELLGLSGVPRAGEVLNAVADERASKELVEHR
                     RLRRIERDRAKAGPVSLDKLMDAMKAGLKELKIILKADVQGSSEALGSALGKLSTKEV
                     KVSIIQSGVGGITESDVNLAKAGNAIIIGFHVRPAGKAAALAEQEGVDIKLYDIIYDA
                     LDDVKLAMAGLLAPVKRDKFLGKAEVREIFNIPKVGIVAGCSVVEGTMKRSAMARLIR
                     DSVLIYSGKLGSLRRFKDDVREVQQGYECGMSIDGYLDIKVGDVIESYEVEEIAPTLA
                     "
     gene            complement(20732..21088)
                     /locus_tag="PKL17_07355"
     CDS             complement(20732..21088)
                     /locus_tag="PKL17_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNK44581.1"
                     /translation="MVTSPKKSQTANAQRQTPVRTCVGCRQTDSQATLLRAVIDGAGR
                     LSFGLGRRQAGRGTYVHRIARCIDAAVKGGFARSLRRTVQVGDVAALYQLAAVSDGSQ
                     PGSRAPALSLRNQDNH"
     gene            complement(21114..22736)
                     /gene="nusA"
                     /locus_tag="PKL17_07360"
     CDS             complement(21114..22736)
                     /gene="nusA"
                     /locus_tag="PKL17_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNK44582.1"
                     /translation="MQPNLSMVLEQVGKDKGIDKKVLVQTLEQAIMTAAKKVFGLERE
                     MEAQFNEDSGQVDLFQVCIVTDSVDVKGRDIAKVDADRNGLRADIGDELLFQIFYTDG
                     DADKAEEQQRKYGDILDLHDTVKRFGRIAAQTAKQVIIQRIREAERENTFNEYRDRKG
                     ELIHGTVRRFERGNIIVDLGKTEAILPVREQVQRENYRIGDRIIAYCVDIEKNARGPQ
                     IVLSRASKGLLEKLFEQEVSEIYEKIVRIESSAREPGSRSKIAVSSRDRDVDPVGACV
                     GIKGSRVQAVVQELRGEKIDIVPYSEDPARFVCNAIAPAEVSRVVIDAENHTMELIVP
                     DDKLSLAIGKKGQNVRLASQLTGWRIDIHAESRVREMEQVERQVLAGLDGSSEELSST
                     LFRLGWRSVSDLAEARIEDLRNVPGVGGEAGAIRLKEAALGFVNEERRKRGEPPLSPS
                     ERPARSDLPSSGAARQEAPSRSAPPSRGPQNPLPGIDPDIVARLMDAGYTTLESMEEE
                     DEERLAQAADLTLDEARDVKRRVTRLLTRGNR"
     gene            complement(22806..23321)
                     /gene="rimP"
                     /locus_tag="PKL17_07365"
     CDS             complement(22806..23321)
                     /gene="rimP"
                     /locus_tag="PKL17_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNK44583.1"
                     /translation="MKPVDLKALHKLIEPAVDGQGYELVDLEWTRGVSGWVLRVTIDR
                     LPEQGHVSHQDCVAVSREVSALLDVHDVLPGAYSLEVSSPGVDRPLKKSADFARFIGK
                     KAKVRLRPDAVQSFDLQGPQRPDSVPRRNFSGTIDAVEGDRVKISVEGAGQFELVIGE
                     IEKANAVFEFV"
     gene            23597..24704
                     /gene="prfB"
                     /locus_tag="PKL17_07370"
     CDS             join(23597..23674,23676..24704)
                     /gene="prfB"
                     /locus_tag="PKL17_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139800744.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNK44584.1"
                     /translation="MALETQKTHELLRSLGERLAALRGHLDIEKLQERLLEIEQVSAD
                     PAFWNQQEKAQTILREQAGHKATVGEWSRLSGELQDAKVLLELAEEAGDEGEAEEARK
                     LASRVETGVEAMELRRMLSGEQDHTDALVSINAGAGGVDAQDWADMLRRMYYRWAERR
                     GYAVEELDLQEGEEAGIKSVSFAVRGPNAFGYLKAESGVHRLVRISPFDSNARRHTAF
                     AAAFVYPEIDDSIEVDIRDEDLEVQTMRSGGAGGQHVNKTESAVRIIHKPSGLVAKCQ
                     SERSQHKNRSTAMKMLRAMLYAQKLREKEAKIDEANSHKKAIEWGSQIRSYVLAPYRL
                     VTDHRSELKVGNVDAVLDGELDEFIKAYLIQAAG"
     gene            24708..25850
                     /locus_tag="PKL17_07375"
     CDS             24708..25850
                     /locus_tag="PKL17_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNK44585.1"
                     /translation="MTIAQSVAERFRADFSLEPDEVLFNNAGVAPLSRVAEAAIHTMA
                     NRMCRGWFALSDVLRLYEQGRIALARLTGARSEDVALFQTCAAAISQVAFGLSLRPGD
                     EIVTVDQEYPSNAYPWHAAAQRASALIRTVRSQPDFSLPTESVIAAIGPQTRVVAVSW
                     VQFQTGAVLDLAALGQACRRHGAWLVVDAIQGLGVLPFDLHKLGVDAVCGGTHKWLCG
                     PLGQGFLALAPGRAAELSPLLHGAMTYGTPDDPVDPSRAPHPDARRFEPGSPQLLPVA
                     ATLATVERSLSYGIENLHQAALAVSDRLITGLATRGARLLLPVGSALRSPIVTFVPRG
                     SLEAATARLSAHKVAFARRAGGIRLSPHAFNHAADVDRFFAILDGA"
     gene            25813..26973
                     /locus_tag="PKL17_07380"
     CDS             25813..26973
                     /locus_tag="PKL17_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44586.1"
                     /translation="MSIDFLPSSTALELFAIGAPGLERALFQELCAICPQAKLVQTPE
                     GCTVVGDLETLYRLNLHSRLSTRVLLRVGKLRALHFPELRRKVAQLPWPQFAKSAVRL
                     RVSASAHRCRLIHTGALIERVELAVADSGVTPTDDPDAPLLHVFVRGQADQFTLSVDT
                     SGELLHRRGYRHEDGGAPLRETLAPALLALANFSDATALCDPMCGSGTLAIEAALVAL
                     RRAPGLQRSFAFFDWPGFSPSLWQKLREESQAAERRTLPQPIWASDRDPKVIEIARRN
                     AERAGIAEYISFAVCAVAQLRLPPVETGLILCNPPYGKRLHDPQLVETYRSLLHLWRR
                     SPGFQLAVFTPSAKLAAAAGTKTCLATLHNGGLRVGLYGSEPVRESAAVTLF"
     gene            complement(26960..27616)
                     /locus_tag="PKL17_07385"
     CDS             complement(26960..27616)
                     /locus_tag="PKL17_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44587.1"
                     /translation="MTEQELTLAEQTLHYDLKLNINIPMTYQGLKISCQNLFGIQCSA
                     LPGVLGLTSSYQVTGLCDDKTMLCAGDIKQLILFVVDFSKDPAFTTGFAQSNADNMRD
                     VNLFYTASNGVDIDITKIDAYIGPDGIRSTTDPRAIYIGSVGPLPRKGSITDNGSPIP
                     VPDGSPAHDVFINAVRDPSKPLNVLLSVTARYISGDPVPAAGAIDVKLTPRVKMLKKE
                     "
     gene            complement(27827..28183)
                     /locus_tag="PKL17_07390"
     CDS             complement(27827..28183)
                     /locus_tag="PKL17_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44588.1"
                     /translation="MFRDSLRTLVEKTDGAVAGLLMGFDGIAVESFAKDAQKLDIQTV
                     GMEFSFILAQVKKAADIIEGGSVEEISIRTERMTLLIRVISKDYFVALALAPSGNSGK
                     GRYLLRVTAPKIQAEL"
     gene            28368..>28679
                     /locus_tag="PKL17_07395"
     CDS             28368..>28679
                     /locus_tag="PKL17_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44589.1"
                     /translation="MTISPAEAQTITDPRIATHLIGPSGDPLGPGGDPLAATALANTL
                     PSPGPSASTGKPGESGQPATTVRASGAQRSGFSSSLLDCDIPPPAEVEGYEIKKPLGR
                     GA"
BASE COUNT         5618 a         9054 c         8375 g         5632 t
ORIGIN      
        1 ccagcgcacg cgccacgcac tggagcagct cttgcggctg aaacggaaac ttcgaaatgt
       61 agtcgtaggc gccttgctga atcgctttca tcgcgttttc aacatcgcca aacgccgtca
      121 tcaaaatcac cggtgcctgc ggctggtgtt tctgaaacgc ggaaagcacg tcgagcccgt
      181 ccggcccata cccaagccgc acgtcgctga tgatcacatc gaaaggaagt tcgctggcga
      241 gctggatggc tttgtcgccg ctctcgcaca gggtcacgcg gtagccggcg ccttgcagaa
      301 actctccgac cactttgcgg aactgttcct cgtcctcgac caccagcgca tgcgatgaag
      361 ccatggctgc accatagcat gcgcggcgga gagccggcgg cagcgattac ttggcagcgg
      421 gtttggcggg cgcttttccg acgggtgctt tggctgccgg cttgaccggg gctttgccga
      481 cgggcgcttt gacgggcgct ttgccgacgg gcgctttcgg tttcgcttcg tcttgcactg
      541 gcggcggtgg cagcggcgct ttcgccatcg gcaggttcgg ttcgacgatc acgaaatcga
      601 cgcggcggtt gagcgcccga cccttgacgg tcttgttgtc ggcaatcggc ttgctcgggc
      661 caaagccctg cgaatccagt cgacgggcct cgatgccctt gacgataagg taggccatca
      721 cgctcttggc gcggcgatcc gacaagctgg tgttgtaatc ggccttgcct tggttgtcgg
      781 tgtgaccctc gacgcgcact ctctttatgt cggggaacgc cttgagcgcc atcaccaccg
      841 cgtcgagcac cggatagctg acctttagga tggtgtcttt gcccgtcgca aagaacactt
      901 gttttaggat cacaatctgg ccgctttcga cgacgaccag gccggggcag ccgttctttt
      961 tcggatctgc cgacggtgcg ccttttttgt cggggcaggc atcaacagga tcggcgatcg
     1021 agtcgccatc gcgatccggc gacgggcagc ccgctttcat cgggtcgggc atcagtccag
     1081 cgtggacatc tttgcactga tcttggctat cgaacacgcc atcgccgtcg ctgtctttgt
     1141 ccgggcagcc tttgcggttc ggatcaggaa ctttgcccac gggttcagat ggacacaggt
     1201 cttccggatc gagcacaccg tcggaatccg cgtcgcccgc cgggcagccc aagcgcttcg
     1261 gatcgggaac ttttcctgcg gcttccgtcg ggcacagatc ctgcggatca agcacaccgt
     1321 cgccgtcttt gtcgcccagc ggacagccgc gtcgctctgg atcgggattt ttgcccgccg
     1381 gcacgtcgac gcagaggtca tcgggatcga tcacgccatc gcgatctcga tcgttgggca
     1441 gcggacagcc gtgctttttg ggatctttgc tcgcgacgcc ggcttcgctg ggacaggcat
     1501 ccgagtgatc gggaatgcca tcgccgtcgg catcgggcgc tttcgcatcg cggaacggcg
     1561 cataagccag ccgcgtcagg aaacggaagt ccggtgcgcc tggaacttgg aaaaacgtcg
     1621 tcccaccgcc gagagaaatt tgcatcgtct tggccaggct gtagtgcgcg ccgagcaaca
     1681 gctcgattcc catcgagtcc ggcttaaacg cccgcgatcc accggctgaa tcggcagcga
     1741 ccaccgacga aaccaggact tccggaccga ccgcaagtcc caggctgcga ttggcataag
     1801 ccaccgctgc accaaactgc acttcggcac cgatcgagtt cgagttcacc ggttgcagct
     1861 tcgacagcga cgcatttggc cgccagtaaa agcccgccgt gagcgaccac ataagcgact
     1921 tccaaagacc gcccgcgata agcttcggca gggcgcgcac accactttcc cccgtctgcg
     1981 caggaaatgg gggcggactg gcgaaggtgt ttatcggaat ccacacgtcg acgcccaagc
     2041 tcagcgacgc cggaccttgg tagggctgac caaacagccg aaccctggca ccaatccgcg
     2101 gatctccgac ggcagccccg ctgaccggcg aaacaccgta ctgcgattcg ccgctttcgt
     2161 acatcgtcag cggcatcgac aaagacagaa gcacgcggtc taaaaacgat cccgccacgt
     2221 cgatgtgacc aatcagctga ttgccgatca ccgccccgcc ccgcacaaac gaaccgtcgg
     2281 caaagcgcag cccatacgtc agcggattgt gtgcgtagtt tagcgtcacg cccgccgcga
     2341 aatagcgggt gctgctgtac cacgggtgct cgacgagcag cgaccactca ccggcggctg
     2401 ttggttcata acggttgacc tggaatccag ggcccgcagt ctgagcgctc gccgaggctg
     2461 aaacggcagt tgtccagagc aaagcaatgg caggcaagag ttttcgcacg atctacccct
     2521 aacggttgcg gaaaagccga cctctgcgaa gccggcgcac atgacgaagc gcggtggaaa
     2581 acagcagcac cgcagacccg aacaaaagcg aacttttcgt cgctggaaaa gcttgtgtaa
     2641 ccccacagcc aaagccgccg cccgcaagct gcgcgggccg gtacggcgca agcgatgagt
     2701 catactgtac cgtcgctgtg gcttggttgt tgcctggcgt atcatcgccg accgaaccac
     2761 ggactgaatc agcaaatgtt agcgccgaag gactgtccac cggctccagc acgaggagca
     2821 cgtcatcggt gccaaagcgg gcaagctgcg ccgccgtaca ctcgtaaccc gtttcggcgc
     2881 tgccgctgca cgtcatcgtc gctgagcttg gctcgcgcag cacgcgaaat ccgtcgggaa
     2941 tcacaaaccg cagcacgatg tcgcgggcgt cgctcgggcc catgttgcgc gcctgcgcca
     3001 agaccgaaat cggctgtccc ggtgcggcgg gctgcggagc catcgtcacc agcgcttgca
     3061 gatccgcgac gggctgaatc ggcacgctca gcgatgcgac gttgttgctc agatcgacat
     3121 cgctctgccc gctcgccgcc gccaccgtcg cttgataggt cagggttccg gtcagcgccg
     3181 acggcaccgc gatcaccacc tgatagaccg ctttttgccc cgtcgacagc gtgacggtcg
     3241 aattcagatc ctgaaagccg cgattgggat tgcagcttgc gacatcaccc accgtcgcag
     3301 gaatcgccga gcaggaccag ttcaccgatg gaatccccaa aggggacagc aggctgctca
     3361 gcggcgctgc gataaccgtt tcactgccca ggttttgcac cgtgacgctg atcgtgtagc
     3421 taagccctgg cactgcgctt ttttgcgtgc tcgtcaccgc aatcgaaagg tctgttggca
     3481 tgcagccgga aagcgcgctg cccggaacaa acacgatctc ttccccgtcg ctgccgcggg
     3541 ttgcgccatt gaccggaaat tcgcttacag ccgccgcgag tgtcgtgccc gccgccgcgc
     3601 ccgccgtcga tcgagcaacc gtcaccggca gagtgcctgg caaaaccaac acgccgccac
     3661 cgccgccacc accaggaccc gccgcatccg tccccgccgg ggccccctgg ttgccaccga
     3721 gtccgcccgc cgcttgcagt ttgagcgagc cttccagcac agggacaaaa agcgccagcg
     3781 tgccaccgcc accgccgccg ccgcccgctt gatcgtgcga cggaccactg gtgctgccgc
     3841 cggggagacc gctcgcgctg atccgaccgg caccgcttaa ccgactgccc cacaaaaacg
     3901 cgagcccgcc gccgctgccc cccggagcgc ccgccgctgc ggtcttgccg ctggcatcgc
     3961 cgccgccgcc gccaccgccg aaaaacagcc gactgccggg cgtttggacg atcggatgac
     4021 cgccacgacc accccgctcg cgcctttggt tgccgcccca actggcggtg cccggaccga
     4081 cgaccagcgc atcctgatcg ctgctgctgc ccgaatatcc gccgcggcca ccgcccgcag
     4141 atgtcgtcag cttgcccgcc aacatcgccg ctggatcgag attccacgcg gcttttccga
     4201 cggcattcgc atccatgaca ccgctgccgt catacgccaa ggccgccccg ccatttgcac
     4261 cgccaccgcc accgcttttg acgccgtttc caccaccgcc gccgttggcc ggtgcgccgc
     4321 gaccgaatgc accacctagc gcggcatagt cggcagaaaa accggcaatg ctttcgcctt
     4381 tggccgcccc gagcgaagcc tgcgtcgtgc gataacctgc tcgatctgga ctttcgtcgg
     4441 tctgcaccac aagacccgtc tgaccgctgc cgccgcggaa accctgacca ctcacatcta
     4501 gtgcgccgtc gatggtgatg tttccagatg cccgcagcgc gacgatgccg cctttttgcc
     4561 catcccacgg ctgggccacg acgctggtgc cgctgggaat ggtcaggttg ccgacctgcg
     4621 gaacccgcac cacctgcgac ttgcccgtcg ccgaatacgc attgcgcagc ccaccgcagc
     4681 gggtatccag cagcacccgc gagctggcca gatcgacgct gcgcactttc acaagttcat
     4741 acagaccggc gccttgcagg ctctgcaccg agccaaactg atcgtcgtcg gtcgatcgaa
     4801 tctgcgcccc ttgcagttcg atgatcagca gcagatcgtc cggtgccaca ttcagcgccg
     4861 ccaggttggt cactgacacc gccgtgtcac ctgccgccgc cgctgccgaa agcggcgcat
     4921 agcgattgac cacttggccc agcatcgtcg ccgtcaagtc cccatcggga ttcgccgcca
     4981 ggttcgccgt ctgcagcgtg ggaacttccg caaaatccgt ggccgcacag ccagcccaaa
     5041 gccccaagag cccaaacatc aggccggctt gccgtcggat cgttgctgcg ggtgaagacg
     5101 ggtgcgacac gatactcagt tgaaattgta tcgctcgcta ttttggattg gcaattacga
     5161 aatatttatc cgcctttagg acacttgcag cccggccacg cggatgactc aagtcagctt
     5221 gcggcaatag tccaggccgt cgcgcaggtt gcgcagcgtc acaagctgcg tcatgtcgac
     5281 ccccgcctgc accatcgctt tcgcaagctg agccgccacc ccggtcacga tcgcctgaga
     5341 tcccaaaagc gcaatcgatc gggtcacgcg aagcaaaagc cgcgccccct catcgtcgag
     5401 gtttgcacac ccggtcagat cgacgatcac aaaccgcgcc cgctgtgccg ataccgcttc
     5461 gagcagccga tgcaaaagct cggccccgcg cacgtcgtga agttcgccga tgatcggcac
     5521 cgcgaggatg ccgcgcccaa catctatcac cggcgccgac aaagccagga tctcttgccg
     5581 ctgggtttcg acaagctgta gcgcgctctg cagctcggcg accaggcgac tgcggctctc
     5641 agccaattcc tcggcttgcc gcctttgcga cacatcgagc tgctgaagca ggacgccccg
     5701 cttgccagtc agcggatcgg tcgccgcctg cgcttcgacc aggtgccagc gatcccccgc
     5761 tgccgtctgc acaggcagct cggcttgata gcgatcacca ctggaaactg tgcgcaggat
     5821 cgtctgccac gtctcgggaa accaggactg aaaccgcgtc tcggcagcaa acgtccgcag
     5881 caccgtcggg ttgtgaaaca gctcgtcccc gttttcatcg agcaagctga ccaaaagcga
     5941 cgtatgacgc agcacctcga cgccgcgcac catcgatcca tcaagcgact gatcttttac
     6001 caccgcctga aacaaaatcc caagccgccc atcgtcgagc gaaacgcccg tgccgtgcag
     6061 cgtcatcgtc gtcggtttgc cgcgcggata cagcgtccag tcttcggcca cttcttcccc
     6121 agaagacacc ttgcgcatgt aactgtcgag ccgcgcctgc gtcgccgccg agttgtcttt
     6181 gtacgatcgc gcatacagct cggcgcgcga ctgcgctttc cacagcagca gcgcttcttg
     6241 attggcccag acaatcttgg cgtgctcgtt gtcatagacc cacaccgcaa gccgagacag
     6301 ctccagtgcg gacagctttt cggacagcat catcctgctt acgatatcag gcgattcact
     6361 tcccagtaaa tccaagctat tatccgcccc tcgacgcacc ttcggagaga gagcccgtga
     6421 agactgaaaa tgacccttgt gcaccgccga ttgcggcttt cggcagcgat tccgccaaat
     6481 cgaacttcat ccgcgacatt atcgatgcgg atctgcaaag cggcaggcac aacaccgtcg
     6541 tcacccgctt ccctcccgaa ccgaacggct acctgcacat cggtcacgcc aagtcgatct
     6601 gcctgaactt tggcatcgcg cgcgattaca aaaaagccgg tcacaccgcg ttttgtcacc
     6661 tgcgcttcga cgacacaaac cccaccaaag aagacatcga gtacgtcgag tcgatccagc
     6721 acgatgtgaa gtggctgggt ttcgactggg gcgacaagat gttttttgcc tcggattact
     6781 ttgaaaagct gtacgacatc gcggtctacc tgatcaaaaa aggcaaggct tacgtcgaca
     6841 gctcgacgct cgaggaaatc cgcgaaggcc gcggcagctt gaccgagccg ggcaaagaca
     6901 gtccgtaccg cagtcgttcc gtggaagaga gtctggatct gtttgcgcgg atgcggaaag
     6961 gcgaattttc cgacggtgcg cacgttcttc gcgccaagat cgacatgagc gcgaaaaaca
     7021 tgctgatgcg cgatccggtg atctatcgga ttcggcacgc gcaccatcat cgcaccggag
     7081 acgcttggtg catctatccg atgtacgact atgcgcatcc gctgtccgac gcgattgagt
     7141 cgatcacgca ctcgatctgt acgctggaat ttgaaaacaa ccgcgagttt tatgactggc
     7201 tggtagcgga agcgcgcgat cttctgccgt ctttgccaca ccagtatgag tttgcgcgac
     7261 tttcgcttgc gtacacgatg atgtcgaagc ggaatctgct caagctcgtc gaagaaaagg
     7321 tcgtttcggg ctgggatgat ccgcgcatgc cgacgattgc aggactgcgc cgccgcggct
     7381 acacccccga agcgattcgc gactttgccg acatgatagg agtcgccaaa gcgaactccc
     7441 aagtcgacta cgaaaagctc gagtactgca ttcgcaacga cctaaactac cgtgcgccgc
     7501 gacggatgtg cgtgctcgat ccgctcaaag tcgtgatcag caactttccg gaaggacagc
     7561 gcgaagaagt cgccgcggcg gactttccgc tcgatgtgaa caaaccggga acgcgcccgc
     7621 tttccttttc gcgtgagatt tacatcgagc gcagcgactt cgaggaacat ccacccaagg
     7681 gattccaccg tctgtcgccg ggtcaggaag tgcggctcct gttttcgtat attatacgtt
     7741 gcgaagatgt aataaaggac gcttccggac acgtcatcga gctgcgctgc agctacgatc
     7801 cacagagccg cagcggtcag tcttcacggc gggtcaaagg caacattcac tgggtggaca
     7861 aagcgtcggc aaagccgtgc gaagtgcgac tttatgaacg tctgtttgtc gcgcagcagc
     7921 ctggtgtgga acgcgatttc cacgaggatc tgaatccgga atcgctgcgc gtcatcaaca
     7981 cggcgcttat cgaacccagc gtcgaaagcg atgaagccgg cagccgctac cagtttgcgc
     8041 gccagggctt tttcgcaagc gatgtcaaag acagccgtcc cggtcacttg gtgtttaacc
     8101 gcatcgtgac gctgcgtgat tcgtgggcca agagcgtaag cgatggcgaa gcggaagaaa
     8161 aaccgacgga aaaagccgcc gccaaaccag agtccgccga aaaggccgac gaccggagtc
     8221 gtccgcagaa aaaaagccgc gcccaaattc gcgccgaagc acgccaaaaa gatccgcagc
     8281 ttgcggcccg ttttgcgcgc tatcgcgacg atctaaagct gggcgaagaa gaagccgaca
     8341 tcctgtccgg ggaagcgctg gtggcagaat tttttgattc cgcgtgcgcc atctacgcca
     8401 atccgcagag catcgccaag tggatcatga gcgagctttt gcgcgcgcaa aaagatcgtc
     8461 ctctgtccga gcttccgctg ggtcctgtgc agcttgcaga gcttgccaag ctggtcgatg
     8521 aaggtgaaat ctcgacgagc gcaggcaaga cggtgtttgc agcgctgatg agcgaagggg
     8581 gctctcccaa agcgctggtg gacaagctgg gactgcacaa agtggccgat cagggcgcgc
     8641 tcgaaccgat tgtggacaaa gttctggccg acaacgcgga caacgtggcg cgctacaaag
     8701 aaggcaaaaa aacgctgctc ggaatgtttg tcggacaggt tctgaaagca acgggtggca
     8761 gtgccgaccc gacgctggcc cgcaaactga tcatcgcccg catcggcgaa tgatcgcgat
     8821 acccacgcga ctcctttcgg cccactgctc ttttccctct ttcggggtct cgctgtgggt
     8881 cccccttccc ttcgagcttt ttttcgtccc gcgagcggtc cgataaaaac tctccggtcg
     8941 gcccactgct ctttttcctc tttcggggtc tcgctgtggg tcccccttcc cttcgagctt
     9001 tttttcgtcc cgcgagcggt ccgataaaaa ctctccggtc ggcccactgc tctttttcct
     9061 ctttcggggt ctcagccact cacttttgcg gttcgacttt tacgtggacg gggagtcctt
     9121 ggcggacgct ctgggtcacg acgcagaagt cttcaaacgt cgccagacat ttttccagcg
     9181 cagcagcatc tccgtcgatt tgcggtcgca gcgtcacatc gactttgccg attcgcagcc
     9241 gcttctgttc gttgcgagtc agttccacat gcacatcgga cttgaaaccc gacagcttgg
     9301 cgccgtttct ggacatgcag aacacaaggc tcgcggacag acagttgcca attgcggcag
     9361 ccaaaatccg cgacggattg ggcgccgtgt ctttgccaag cggcgctggt tcatccatgt
     9421 gtagttcggg atgctgcggc ttgtcgaact tcacccgaaa ttcgtagcca tcgatctgct
     9481 cgatctgtag atcaaacgaa gacacaagct cactcatcgg ttgctccttg tccgtctact
     9541 ttaccgcgcc aaagggaagt ctgcgcgact tatgcgacgg cgattccttc gaaaatggcc
     9601 tgaccagcag gaagcggaat caagcgacgg aaacggaacc cggtcgcttc aaagagcagc
     9661 gccatttctt tttccgtccg ttcccgaccg ctgtaaatca gcatgccagc gatgtccatg
     9721 aacggaatca gcgtcgtcac cgcatccggt ccaccgacaa aatcggcaac cagcagacga
     9781 tgtccgggtt ccatcgccgc gcgcacattg cgcaagatgg tgcaggcgtc ttcatcgttc
     9841 cagttgtgca gcaccgtctt cagaatgtac gcatcagcac cgcgcggaat tgatccaaaa
     9901 aaactgcccg gtgcaagctc aacgcgatca agcacaccac gttttcgcag atgttcgggc
     9961 gctttttcga gcatcgcttc ttcatcaaac agcagcgccg acaagtgtgg atgtcgacgg
    10021 agcgccgcag cgagcacaat tccgacaccg cccccgacat cgcagatttt tttcacttca
    10081 tcaaacggat acgcggcggc aatcgatgat gagacttctt cggtcattga gctcattcct
    10141 tccacaaagg aatcggcacc gactttcgat gtggcaagcc actcccacac accgcgtccg
    10201 tgcacgcgct ggaaaccgac tccgccatgc cgcagtacgt tcggaagatc ctgccatgcg
    10261 tcgacgatct cgggaattcc gaagaactcg acaaagccac gcacgctgcc cggaacgccg
    10321 gtgcgaagtc cctgcgcgac ccggttgttg cggtacttcc cgtcttcact tcgcgcaaag
    10381 acgccgattc cgacaagcgc acgcaacagt cgttcggtaa gcgcaggatc ggtcttgcag
    10441 cgggccgcga tctccgctgc ggtcagcgga tctttggcga gcaagtcggc gatgcgcagc
    10501 tctgcagcag agtgcagaag catcgttgtc gccgcaccaa aaaaccgatc gagcagcgcc
    10561 acatacggtg ggaccacagc atcggcagca cgcagcagaa aacgccgcag ccgcagcgcc
    10621 atctccacca aaaacacagg aggaaccgga ggactttcag gagtcgtttt gtcggcagat
    10681 tgagacatgt gaataccctc cccagaaggg aatcggaaca ggggttcttc gattcactgt
    10741 caatctgcgc acgacttggc aggtaggaaa gagcgtgttt agtcgtgatt gcgcgtgcgc
    10801 agcacaccga caccggggtc ttgggctgga acgttgattt ctttccaggg aaccacacca
    10861 ggggctcgcc aggcagcggc gcgactggtc gacacccaga tcggattggt cagcgcatag
    10921 ggctgcggat tcccaaccac cactgcacca atgtctgtcg tcgccgcaat cccgagcgga
    10981 cccgccagcg cgcccaccgc atcggaaacc aaaaggaatg gctcctccgt tccggtgatg
    11041 acagggaaca tcggtcgatc gcccaccgct tccagcacga tccaggaatc gcgcgatagc
    11101 tgaagcggta tctgtccact ctgtggtccg ccgcgctctg cacggtctga caagttgcga
    11161 tcgggatcga tcggaatccg ctgcacaaga cgaccattta cgtagacatg cagccgcgca
    11221 accgacaccc aaggcggagc cgataggcgg tagttcacgg tgacttttcc gtcgggggcg
    11281 ttgacttccg aaccaatctg caccggttct gtttggccac cgctcgccgt aggaaccgtc
    11341 accgtaaacg tcaaaaacgg tccatgggaa agcgtcactg cgcgctggtt tttgatcaca
    11401 tccaccagct gcgccgcatc cacacgcacc ggatcatcgt gacccacgcg cagatacgtg
    11461 cgcggcgcgc cgggctcttc gccgacgtcg gcatgggaat ccgaagccgc caccacggtg
    11521 tgacggaatc cttggtttag catgtggaac cagtcatcca ccgcaccagg aaacaccgct
    11581 tccacattgt ccagaccact agaccccgga tcagcagaag gagtcttgtc gagaaggatg
    11641 gttccgggca ccagcgcatc ggtcgtgctc gacgggcgca gaatctttcc gtttgcacta
    11701 cacggatcga gcatcgtctt tggatcaaag ccactcggca gcggcttgta gcattccttt
    11761 ggccagtctg tcggcacccg caccgagtga atctgatgtc ccaatttgcc gttcaggatt
    11821 tccagcgcat cgcagccata ggacagtcgc gactttcctt tttcgtcgaa aaatgccctt
    11881 ccgtttggac cggccagccg cttcgacgtt ccttgcgcca ccatgtcgcc cgtcacactc
    11941 gatcgtccgt actggttgaa atagccctgg gtcgaatcgc gtggatggtt gcacacgatg
    12001 atgttggtct gggccgcagg atcggcaagc tgccgcagac ccgcaaacag ctcatcgggc
    12061 ggacggccaa accactcgaa cgcgccgtgg gtcacgggac cgactttgta atcgagcgga
    12121 taggagttga aatggccgct ttccagcgtc gtaaactcga tgcccacaaa cgtcgccagc
    12181 caaggcgtaa gtcccagcgc cgcaagctgg ggccgccagt ctgaaacgaa gttgtgatcg
    12241 gtcgaaatga caacttccag accttcaccc gctgcggacg tcacccgcgg tgtcagcgcc
    12301 atccgtgaat cgatcgagtt ttccgaatgg acatgtccgt ccattgaaac ccagccgctg
    12361 gtatccacca ctcgggtcag ggttcccgtc gcctgcgctg cattcccaag cgcaacagag
    12421 acaggagcag agaccaggtc atattcgaag ccgcggctaa aatagaccaa atagtcgcct
    12481 ggacggactg ggatcgtaac gtgtccgtcc gcaccggtat atgccaccgc ttcgatgtat
    12541 gcgcgctgtc cttccttgct gtctggcagc atgtcggtga agcgattgtc ttcccctgct
    12601 tggagatcga acaccgcacc tgaccgtcgc tgcgcattgg tcaggtcttg cgaatacttg
    12661 ccgaccacac tgactttggc gggcacagga cgcccactgt catccaccac cgcggcctgg
    12721 atgtacgtag ccggcggcag gatcggatcg atgaatgtgc tctcgcccgc cttgatctca
    12781 aacggaacaa atggaccaat tggtctacct tcactctgca cgcgataggc gtagcggcca
    12841 ggcggcagat tgccaaacag attccctgcg tcgtcggggt aatcctggga atagggccgg
    12901 cacagcgtat cttttcccgc ggtctgacac agccgaagcc cggcatccag atagtcatcg
    12961 tccttggcaa agctgctgct gcgcacgccc gattgtggac ccagctcttg atagatcacg
    13021 atctgtccgt gcgaagtggg atcgcccccc tgctgtctgc gcagaatgcc ttgataggtt
    13081 ccggactggg ttttgcgcag cgaaaggaca tcatcgacaa tcgacgcaac atcgccacca
    13141 ccgacgataa agtactgcgt catttcgacg aaacctcccg gcgcgatggt cgtttttacc
    13201 gaatcactaa agacaccgat aaacgaaatc gcggtaaacg gaaccagcag cgaggtgttg
    13261 tcgatcggcg taaacgtgtc tttgtacttc ccgaggttgc gatacgcatc ggcattctgt
    13321 ttgacaaagt ttgtctcgct gggtgcggcg atgagtccgt agcttacgcg gtcgttcatc
    13381 cggctcgatg ccgacgcgat aaactctgtc accaccccgt taaacgccgg caaagggagt
    13441 ccgcgcccaa acgatcgctc gagcggatga cgcagatcaa agccggttcc tggcagccag
    13501 acgttgttga gccgtccgta cagcatcacc attccgatgg gtacgcgcac ggtgctcaga
    13561 tcgacgccgc caatctgctg ctggaacgtg gaatttgcca gcaccgagct aggaatctgc
    13621 agcggttttt tggtcttgtt gatgatgcga gagtgaatct cgacgtgacg gaccgaagga
    13681 cgcagcacgt agtcgacttc atagcgcgcg ttttcgttct gtagtttgtt tagctcggca
    13741 gcaaagtcgg caaaagggag tcccaaaagg agtgccagcc gaaccagttc actgttgcca
    13801 tcggaagagt gtgatgccgt cggtaacaag tagtttagga acaggctgtc gaacactttg
    13861 agcagggtaa gaaactctcc gccaccaccg gaagcacgga tgactgcggt gccttcatcg
    13921 tagtgaacgc ggtttgttcc ataccgttcg tcatacggtt ttgtcccgtc ggtgatgact
    13981 tcgactttgt cgcacgccac cgcttgaaaa aagaacgacg gaaacatctc ggcaaagatg
    14041 tcgttgccgc cttggcgatt ggattcgttg cggccttgtt catcaaaacg gcgcaagtct
    14101 gcgtcgatga ttccgccgcc atagactccg aagccgcgcg aaaatccggc attttgaacc
    14161 accagccgga tgcgatcgtt ttgcagtacg taatcgccga cttcaccgag cgcgcgtgga
    14221 ccaccgccaa agagctgatt ccgatccgtg acccggaatg cccgcagaag cgacgattca
    14281 ctttcgcgct gacaggtggc cagcgaaacg atcgacaaaa gacaaaacgc aaacacccag
    14341 attcgcttca tgggaagact ccttttgtgc tgtcttcaca cggactaaaa cgcagccaca
    14401 atgtccgctt caaagcgatc gttgtttagg atgttgggcg cttgctctgt ggtgatggtg
    14461 tagtttagcg acatgtgaac ggggaacgtt ctcgtcggac ggacgaagat tccgaacgtg
    14521 tggtacagaa gtcccagtgc agcatccact tgcttgtcca tgtcgcttgt tggacttgtc
    14581 gaaagatgcg cccgcggtga ataggaagcg atgcggtagc ccggtgtgat ctcgaccgga
    14641 atgcgcggca aggtgtagcg cagatgcgcc caaaaaccga agcgctccag cggaggaacc
    14701 gcgctcaacg tatcgcgcga ggtctgcatg tacaaaacgg cagcttggag ttcgattcca
    14761 aacagcgacg ctgccacatc tgcggccact ccgacatcgg tttcacggat cagatcgggc
    14821 aaatttccga cggtgcgagg attccactgc gctgcgattc ccagatgcaa aaccggcttg
    14881 cgcagatcat ctgtcaccgc ataaatgggt cgcacctgat cgtttgggag tccctgtgtt
    14941 ccccaatagg aaaactcggc gcgaccccgc accgccggaa tgttgttgtc ggacagaatc
    15001 tgattctgtc cattcccatt gccggcaaac agcgcgtagc gaaacgaggt atttcccgcg
    15061 agcggaatgt gtcccgagtg aatcgaaatt cccagcgcac gatccgatcc cattccgcgc
    15121 gttgtatagc cttcggtcgg ctgcacgcct tccaccggca ctgcgcgcga cacaaacggc
    15181 agctcggcat cggagcgcat gctgtccaag tcccacggcg tcaccatctg cccaacgctg
    15241 acgcggaaaa accggaacgg cgcccaggtg atataggcgt cgcgcagtcg tgccgtaagc
    15301 tgaccgccgg gaaagctctg gcttaggcga tcttcgctcg cgccttcgat cgaaacccgc
    15361 gctgccagca gggacttgta ctgcacgttg gcactgaccc gcgcttgctc cagctgaaag
    15421 ccgttgttgc gtcccgcgag gttgtagttc tgcggctgct cgatccagcg gtacgcgccg
    15481 tggatgtagc cgcccaggtc aatgcgccaa atcgacgcgg cgcccatcgg catggtgaca
    15541 gacggaatca gcgtcggtgc gggaccgggc gcaatcgctg gcagcgcgct ggttccgtct
    15601 tgggcaggca cgggctggac ggcaggctcg gggagagccg gtggtggcgc atcgacaggc
    15661 ggcggggaaa cgtggtcgcc atagctttgg gccgacgccg caaatggaaa cgaagacagc
    15721 acaagcgcgc aggcaagcga agcgggagag gatcgcaaca cggagtacct ccgaccaagg
    15781 cgcagcgagg tagccaccgc ctcggacaac acgatgtacg agcgaaaact acagacccac
    15841 gcgcagagga acccacacca ccgcaccctg tcaagcggtg gttcgatggt ctgcgcaggt
    15901 gttgcgaaat cggcggcggt tgttggggcg gtcgaactta acggctgggt atcagccact
    15961 gcgccagctg gcgaaaatca agcggctgcc ccgcttcaag tcgctgatgc tgcaggacca
    16021 gcgcgtgcaa aaacagaagc tgctcgtcga gctgggcggc atacagtgcc gactgctgct
    16081 cgacccgacg cgaaaggagc tgcaccaccg cagcgcgcag gtttttttgg tccgcttcag
    16141 gcagtccgcg tgtcttctgc ggctgccagt ccagctcccc atacgaaagt ccgagcccgc
    16201 ttagcacaga cccaagcagt tccgtcggca aaagcacgcc gtggcgctga tacagcacct
    16261 tggcgacact cgcgcgcgcc cgcagctgct gtttttgcat cgacagatac agcggctttt
    16321 ccgactcgac cagctccaac acctggccga gcagtccgcg cagtcgctca ctcgcccgct
    16381 gaagctgctg cgacggactc agttttttgc gcggtgcctg cgctggcaac ggatcgctcg
    16441 gcttgggaat cgcggcgctg ggcagcggcg gacagcctgg ctcaagcggc gctgccaagg
    16501 caaactcgct cgggtccaag tcgacaagca ggttgtcgaa cacttcgccc gaccagcgat
    16561 cggcaccttc ttgtgccagc aaaatgtagt gctcatacgc gcgcccaagc cgcacaagca
    16621 gcgcttttcg cagccgcagg ctctgctgca ccgattcgat ctggcacagc gtgtcgtggg
    16681 aaagtggcgc ggtgcccaaa agcggcacgc tcaacgtctg gctgtcgaca tacgcgccaa
    16741 gcccggatcg cagctcatca agcgaggccg acagccgccg cgtcgactgc accgcttgct
    16801 gcgacagtcg caccgcgctg tgctgattca ccgtcacaca gcccgacaga cctgcgagcg
    16861 cgacaagcca cagcacacag cacagcaggc ctcgcttcac ggcctagcgt ccccctttgg
    16921 aacgcggcga gacatgcgac tccggatttt gcgacggact ttccggatct ggcgaatccc
    16981 acggatactc tgccggttcg agcagcttgg gcgcttgcag cttggggcca gcctttggcg
    17041 gcggcggctc tcctggctcc cagtgctcat agtcgccccc gagcgcgttt tcgaccgcac
    17101 cgtcgccata atcaagaaag tcctgctcgt cgtcggtgat ccgcgcaaac gccagatcgt
    17161 cgataaactg gaggattttc tgcacctgcg actgggtgta gccgcgctgg tttgacacca
    17221 ccacgatgcc aagctgcgcg ctgtcgagcg attcggtgtc gccgacctcg gcaatggcac
    17281 agttccattt ttggtggacg cgatccttga tgcgacgcag caccgagcgc ttgtctttgt
    17341 cggagtggca gtcttcaagc tgcaaggtga tgcgacagac accaacgatc atggcttagg
    17401 accaggaaga atcttaacgc gagccggcca ggcagcgtgc tggccttgcc gcaaacggag
    17461 ggattttagg cgagcgtcgg cgcgatctct tcgacttcat acgactcgat gacgtcgccg
    17521 accttgatgt cgagatagcc gtcgatcgac ataccgcact cgtagccctg ctgcacttcg
    17581 cggacgtcgt ctttgaagcg acgcagcgaa cccagcttgc ccgagtagat cagcaccgag
    17641 tcgcgaatca gtcgtgccat cgccgagcgc ttcattgtgc cttcgaccac cgagcagccc
    17701 gcgacgatac caaccttcgg aatgttgaag atttcgcgga cttcggcttt gccgaggaac
    17761 ttgtcgcgct tgaccggcgc caaaaggccc gccatcgcca gtttcacgtc gtcgagtgcg
    17821 tcgtagatga tgtcgtacag cttgatgtcg acaccctctt gctcggcgag cgctgccgcc
    17881 ttgccagcgg gacggacgtg gaagccgatg atgatcgcgt tgccggcttt ggccaggttg
    17941 acgtcgcttt cggtgatacc gccgacgccc gactgaatga tcgatacctt gacctctttg
    18001 gtcgacagct tgccaagtgc actgccgagc gcttcgctcg atccctgcac gtcggccttg
    18061 aggatgatct tcagttcctt gagacccgcc ttcatggcgt ccatcagctt gtcgagcgat
    18121 accggacctg ccttggcgcg atcgcgttcg atgcgacgca gacgacggtg ttcgaccagt
    18181 tccttcgagg cgcgctcgtc ggccactgca ttgagcactt cacccgcgcg cggcacaccc
    18241 gaaagaccga gcagctcgac cggcgtcgat ggacccgcct gcgccagcgt ccgacctttg
    18301 tcgtcgctca tcgcgcggac tttgcccatt tcttcgccga ccaccacggt atcgccgacg
    18361 cgcagcgtac cttcttgcac cagcaccgtc gcgaccggac cacgcttggc gtccatccgc
    18421 gcttcgatga ctgcgccctt ggcggccttg tttgggttgg cccgcagctc gagcacttcc
    18481 gactgcagcg ccagcatttc gagcagcctg tcgacaccga tcttggtccg cgccgacaca
    18541 tcgcagaaaa tcgtctcgcc gccccactct tccgatgtca ggccgtgcgc cgcaagctgc
    18601 tgccgaatcc gatccggctg cgcgccttgc ttgtcgatct tgttgaccgc gacgatgacc
    18661 ggaaccttgg catccttggc gtggttcagc gcttcgacgg tctgcggcat gatgccgtcg
    18721 tccgccgcga ccaccagaac gacgatgtcc gtcacctggg caccacgcgc acgcatttcg
    18781 gtaaaggctt cgtgacccgg cgtatccagg aacacgacat cgcccgcctg cgacgtaact
    18841 ttgtacgcgc cgatgtgctg ggtaatgccg cccgcttcac cggctgcgac gttggcattg
    18901 cgaatcgcat cgagcagcga ggtcttaccg tggtcgacgt gacccatgat ggtaatgacc
    18961 ggcgcgcgtg gcttcagatc ctcgggtttg tcggtggtct cgacgacaac gctctgctct
    19021 tggaacgccg tcgactcgac acgccactcg aactcactgg cgacaagctg cgcggtgtca
    19081 aagtcgatcg aggagttgat catgacgttg cgcatgccaa gctcccagat tttcttgagc
    19141 acgtcggtgg ccttttgggc catctggcgc gcaagctcgg cgatgacgat cgtctcgccc
    19201 atcttgacga cgcgcttgtg ctcggcggcg gtggtgatct gggtcttgcc gggcttgcga
    19261 ccgggcggca gtttgccacg cgctggctga accggaccaa ccggcgcgcg ccgtgactct
    19321 tggccaccac gcaaccgctc gcccgagact tcctgacgcg gaccgcgacc catttcgggc
    19381 gtatcggtac gctcgccccc cgaacggaag ttgcgcggac tctcgctgct tggcgcagca
    19441 ggcttggccg atggctgggg cttgaacgcc gacagatcga tcacccgacc cgttggaccc
    19501 agtccttcga gcgagcgtcg ggtctgcgcg gtgacaaata ccttggccgg agcaccttgc
    19561 gatggcgaag ttgccgacgg tgccgaagcc gaagctgcac gaactggctc tgcaggcggc
    19621 ggcgccaccg caggctcgac gtgtcgcggg gccgccgatt tggctgccgt ttcgaccacc
    19681 ggtgcagcgg gggctggccg actgaccacg ccctgcgact tcgcttgagc agccggggtc
    19741 gcaacggact tggcggcttc ggccacagcc ggttttggag ccacctgtac aaccggcttg
    19801 gcggctgcag gtttgctctc cacgggtgct ggcttggcag cttcaacctt cgccgccgga
    19861 gccgcaggtg cttccacctt ggcgacgggc ggtgcttcga ccgccttggc cgcgaccggc
    19921 ttgctttcca cgggtgctgg cttggcttcc accacgggcg gcttggccac cactggcgtc
    19981 ggtgcagccg gtgcggcttc caccttgacc ggagccgggg tttccgttgc aacgggcttt
    20041 tcagccacca catctgcttt ggccggggcc aaaggttcag aagcagcctt ggcacgcgcc
    20101 ggctttttgg ccggatcgcc agctttcgca gtcgattcca cagtcggtgc agccacagga
    20161 gccgcggcag gtgtcgtagc aaccactggg gccggcgtcg gatcaaccgg cttggctttg
    20221 acagccacgg gctcgacctt gaccgcagcg ggcttttcgc tgacaacctg ggcaggcggg
    20281 ctcggcggcg tgaccaccgg aggcggcgtc ggcttgacat cggccaccgg acgaaccggg
    20341 gtttccggtg caggactgac cacgcggaca atccgccgag gcggctcact ctgcacgaca
    20401 acgggtggcg gcctggtttc aaccacaggt ggcggcggcg ggtttgccac ggcatgctgc
    20461 gcttctggat gcaagcgact accgtctttc gcccgtcgac gaatcacggt ctccgagacg
    20521 cgctcttgca ccgtgttgtc gtgacgctct ttgtcgaacg cgcgccggac gcggtctacc
    20581 tcatcgcgct ccagccgcga catgtggttc ttggcctcga tgcccaaggc tcggaccttt
    20641 tcgaccaaat ccttgtgggc aatccctagc tctttggcta gttcgtagat tgcgaccttg
    20701 gagcccgcag taccgacgct ctcggtgttc attagtggtt gtcctgattc ctaaggctca
    20761 gcgcaggggc ccgcgagccc ggctgcgagc cgtctgagac ggcggcaagt tggtacagcg
    20821 ctgcgacatc cccaacctgg accgtgcgcc gcagcgaccg cgcaaatccg cctttaactg
    20881 ctgcatcgat acatcgggcg atccgatgca cgtaagtccc tcgacccgct tgccgacgcc
    20941 caagcccaaa cgaaagacgc cctgcaccat ctatcaccgc acgcaaaaga gtcgcctggc
    21001 tgtcggtttg ccgacaaccg acacacgtcc gcactggcgt ttgcctttgc gcgtttgccg
    21061 tctggctctt ttttggcgat gtcaccatgg acaggtcttt ttctccacgc ggactagcgg
    21121 ttgccccgcg tcaaaagtcg cgtcactcga cgcttcacat cgcgtgcttc atcgagggtc
    21181 aggtcagccg cctgggccag ccgttcctcg tcttcttctt ccatcgattc cagcgtcgta
    21241 tagcccgcat ccatcagtcg cgcaacgatg tccggatcaa taccgggcag cggattttgc
    21301 ggcccacgcg acggtggcgc actgcgactt ggggcttctt gacgggccgc gccgctcgac
    21361 ggcagatcgg accgcgctgg acgctcactt ggcgatagtg gcggctcacc gcgcttgcga
    21421 cgctcttcgt tgacgaaacc gagcgccgcc tctttgagac gaatcgcccc ggcttcaccg
    21481 ccgacgcccg gcacgttgcg caggtcttcg atccgcgcct cggccagatc cgacaccgaa
    21541 cgccagccca aacggaacag cgtgctcgac agttcttccg acgagccatc cagacccgcg
    21601 agcacttgtc gttcgacctg ttccatctcg cgcacgcggc tttcggcgtg gatatcaatc
    21661 cgccagccgg taagctgcga cgccagacga acgttttgac cctttttgcc gatggccagg
    21721 ctcagtttgt cgtcgggaac aatcagctcc atcgtgtggt tttcggcgtc aatgacgacg
    21781 cgcgatacct cggcaggagc aatcgcattg caaacaaacc gcgccggatc ttcgctgtac
    21841 ggaacgatgt cgatcttttc gccgcgcagc tcttgcacca ccgcctggac gcgcgaacct
    21901 ttgattccga cgcaagcgcc caccggatcc acgtcacgat cgcgtgagct tacggcgatt
    21961 ttgctgcgcg agcccggctc acgcgccgac gactcgatgc ggacgatttt ttcgtaaatt
    22021 tcgctgactt cttgctcgaa cagtttttcg agcaggccct tcgacgcccg cgacagcaca
    22081 atctgcggac cgcgcgcgtt tttctcgatg tcgacgcagt acgcaatgat gcggtcgccg
    22141 atgcggtaat tttcgcgctg aacttgctca cgcaccggca agatcgcctc ggttttgcca
    22201 aggtcgacga tgatgttgcc gcgctcgaag cgacggacgg tgccgtggat cagctcgcct
    22261 ttgcgatcgc ggtattcgtt gaaggtgttt tcgcgctcag cctcgcgaat gcgctggatg
    22321 atgacctgtt tggcggtctg cgcggcgatg cggccaaatc gcttgacggt gtcgtgcagg
    22381 tcgaggatgt cgccgtattt gcgctgctgc tcttcggcct tgtcggcgtc cccatcggtg
    22441 tagaaaatct ggaacagcag ctcgtcgcca atgtcggcgc gcagcccgtt gcggtcggca
    22501 tcgactttgg cgatgtcgcg acctttcacg tcgacggaat cggtgacaat gcagacctgg
    22561 aacaggtcca cctgcccgct gtcctcgttg aactgagcct ccatctcgcg ctcgaggcca
    22621 aagaccttct tggcagcggt catgatggct tgctcgagcg tctggacaag gaccttcttg
    22681 tcgatccctt tgtccttgcc tacttgctcc agcaccatcg agaggttggg ttgcatcgcg
    22741 tttaccttac ttcctggcct atgccttgtg gccgattcgt cctggcccgt gtgtttctac
    22801 ttccgtcaga cgaactcaaa caccgcgttg gctttttcta tttcaccgat cacaagttca
    22861 aattggccgg cgccttcgac cgatatcttc acgcgatccc cttcgaccgc atcgatcgtg
    22921 ccgctaaagt tccgccgagg aaccgaatca ggtcgctgcg gaccttgcag atcgaaagac
    22981 tgcacagcgt cgggacgcag ccgaaccttg gcttttttgc cgatgaagcg ggcaaagtcg
    23041 gcgctctttt tcagaggccg atcgacgccc ggtgagctga cttccaagct gtaagccccc
    23101 ggcaatacat cgtggacatc aagcagcgct gacacttcgc gcgacaccgc cacgcagtct
    23161 tggtgcgaga cgtggccctg ctccggcagc cgatcgatgg tcacgcgcag cacccatccc
    23221 gacacgccgc gggtccattc cagatcgaca agctcgtagc cctggccatc aacggcgggc
    23281 tcgatcagct tgtgcagggc tttgaggtca acgggcttca tggcttcttt caaaaaaaaa
    23341 ggcgggtccg ggcggaccca ccttccgtgg attcctacca aaatgtatct ccgcctatac
    23401 cagaaccacg gcctttcgcg caagagcgaa gctcgtcacc gacgggacgc ttgcagccca
    23461 aaacgagcga ttaagatcgc aaaaaatcgc cgtctttttg gtccgaccat gatacgagtt
    23521 gggcgattgg ctgcccgatt tttgccaaag cacgcccggc cagcctggtt tttgtgttag
    23581 ttccttccca cttcacatgg cgttagagac acaaaagacc cacgaacttc tacggtccct
    23641 tggggagcgg ctcgctgctc tccgagggca tctttgacat cgaaaagctg caagaacggc
    23701 tgttagagat cgagcaagtc tcggcggacc cggcgttttg gaaccagcag gaaaaagcgc
    23761 agaccatcct gcgggaacaa gcaggacaca aagccactgt cggtgaatgg tcgcgcctgt
    23821 cgggtgagct gcaagatgcc aaggtgcttt tggagctggc ggaagaagcc ggcgacgaag
    23881 gcgaagccga agaagcgcgc aagctggcaa gccgcgtcga aacaggcgtc gaagcgatgg
    23941 aacttcggcg catgctgtcc ggcgagcaag atcacaccga tgccttggtt tcgataaacg
    24001 ctggtgctgg tggcgtcgac gcgcaggact gggccgacat gctgcgccgg atgtactacc
    24061 gctgggcgga acgacgtggc tatgccgtcg aagagctcga tctgcaagag ggcgaagaag
    24121 ccggcatcaa gtcagtgagc ttcgcggtgc gcggcccaaa tgcgttcggc tatctgaaag
    24181 cggaaagtgg cgtccatcgg ctggtgcgga tttcgccgtt tgactcgaat gcccgtcggc
    24241 acaccgcgtt tgcggcggcg tttgtgtatc cggaaatcga cgattcgatt gaagtcgaca
    24301 tccgcgacga agacttggaa gtgcagacga tgcgatcggg cggcgctggc ggccagcacg
    24361 tcaacaagac cgaaagcgcg gtgcggatca tccacaaacc gagcggtctc gtcgccaagt
    24421 gtcagtccga acgatcgcaa cataagaacc gctcgacggc gatgaagatg ctacgggcga
    24481 tgctgtatgc acagaagctg cgcgaaaaag aagccaagat cgacgaagcc aacagccaca
    24541 agaaagcgat cgagtggggc agccaaattc gatcgtatgt gctggcgccg tatcgtctgg
    24601 tgaccgatca ccgcagtgaa ctcaaagtcg gcaacgtcga cgcggttctc gacggagagc
    24661 tggacgagtt catcaaagcc tatctgatac aggccgcagg ataagccatg acgattgccc
    24721 agtccgtcgc agagcgcttt cgggccgact tttcgcttga accagacgaa gtgcttttca
    24781 ataacgccgg ggtcgcaccg ctgagccgag ttgccgaggc tgcgatccac accatggcga
    24841 atcggatgtg ccgtggctgg tttgcgcttt ccgacgtact gcggctatac gaacaggggc
    24901 ggattgccct tgcccgcctg acgggcgcgc gatccgaaga cgtggcgctg ttccaaactt
    24961 gtgcggcggc gatctcgcag gtggcgttcg gtctgtcgct gcggcctggc gatgagatcg
    25021 tcaccgtcga ccaagagtat ccatcgaacg cctatccgtg gcacgcagcc gcacagcggg
    25081 cgtcggcgct gattcggacc gttcgatccc aaccagactt tagcttgccg acggaaagtg
    25141 tcatcgcggc gattggtccg cagacgcgcg tcgtcgcagt gtcgtgggtg cagttccaga
    25201 cgggtgcggt gctggatttg gcggcgcttg gccaggcttg tcggcggcat ggagcctggc
    25261 tggtcgtcga cgccatccag ggactgggcg tgctgccgtt cgatctgcac aagctcggcg
    25321 tcgatgcggt ctgcggcgga acccacaagt ggctgtgcgg accgcttggt cagggctttt
    25381 tggcgcttgc gcccggtcgc gctgccgagc tttcgccgct tttgcacggc gcgatgacgt
    25441 atggaacccc cgacgacccc gtcgacccaa gccgagcccc gcacccggac gcccgcaggt
    25501 tcgagcccgg atcgccgcag cttctgccgg ttgccgcgac gctggccacc gtcgaaagaa
    25561 gcctgtcgta tgggattgaa aatctgcacc aagccgcgct cgcggtgtct gatcgactga
    25621 tcaccggcct ggccacccgt ggtgctcgat tgcttctgcc cgtcggttcg gcgctgcgct
    25681 cgccgattgt gacatttgtt ccgcgcggct ctctcgaagc ggcgacggcg cgactttcgg
    25741 cgcacaaagt agcgttcgct cggcgagccg gtggaattcg cctgtctccg catgccttca
    25801 atcacgcagc cgatgtcgat cgattttttg ccatcctcga cggcgcttga gctgttcgcg
    25861 atcggtgcgc ccggactgga gcgcgcgctt ttccaggagc tttgcgcgat ttgtccgcaa
    25921 gccaagcttg tgcaaacgcc cgaaggctgc accgtcgtcg gcgacctgga aacgctgtac
    25981 cggctgaatc tgcactctcg gctgtcgacg cgggtgctgc ttcgcgtcgg gaagctgcgc
    26041 gcccttcatt ttccggaact gcgacgcaaa gtcgcgcagc tgccctggcc tcagtttgcc
    26101 aagtcggcgg tgcggctgcg ggtgtcggcg tcggcccatc gctgtcgtct gattcacacc
    26161 ggcgctctca tcgaacgggt cgagctggcc gtcgccgatt ccggggttac gcccaccgac
    26221 gatcccgacg cgccactatt gcatgtcttc gtccgcggcc aggccgatca gttcacgctg
    26281 tccgtcgaca catcgggcga gcttttgcac cgtcggggct accgtcacga agatggcggc
    26341 gcgccgctgc gagagacgct ggcccccgca cttttggcgc tggcgaattt ttccgacgca
    26401 actgcgctgt gtgacccgat gtgtggctca ggaacgcttg cgatcgaggc tgcacttgtt
    26461 gcgctgcgtc gcgcaccggg gctgcagcga tcgtttgcat ttttcgattg gccgggtttt
    26521 tccccgtcgc tgtggcagaa gctgcgagag gaaagccagg ccgccgaacg acggacgcta
    26581 ccgcagccaa tctgggccag cgatcgcgat ccaaaagtca tcgagatcgc ccgccgcaat
    26641 gccgagagag ccgggatcgc cgagtacatt tcatttgcgg tctgcgcggt ggcgcagctg
    26701 cggcttccgc ccgtcgaaac cgggctgatt ttgtgcaatc caccgtatgg aaagcgactg
    26761 catgacccgc agctcgtcga aacctatcga tcccttttgc atctgtggcg acgcagtccg
    26821 gggtttcagt tggcggtctt cacaccatcg gccaagctcg ccgcagccgc cgggaccaaa
    26881 acctgtctcg cgacgctgca caatggcgga ctgcgtgtcg gactgtacgg cagcgagccc
    26941 gtccgagagt ccgccgctgt tactcttttt tgagcatctt gacccgtggc gtaagcttga
    27001 cgtcgatagc gcccgctgcc ggcactggat cacccgagat atagcgcgcc gtcaccgaca
    27061 acagcacatt gagcggcttc gacggatcgc gcacggcgtt tatgaacaca tcgtgcgccg
    27121 gcgagccatc ggggacgggg atcgggctgc cattgtcggt aatcgaacct ttgcgcggaa
    27181 gcggaccgac ggaaccgatg tagatggcgc gcggatcggt ggtgctgcga atgccgtcgg
    27241 ggccgatgta ggcatcaatt ttggtgatat cgatgtcgac accgtttgat gcggtataaa
    27301 acaggttcac atcgcgcatg ttgtcggcgt tcgattgcgc aaatccggtg gtaaacgccg
    27361 gatcttttga aaaatcaacg acaaatagaa tgagctgttt gatatcaccg gcgcatagca
    27421 tcgttttgtc atcgcaaagt ccggtaactt gatacgaact ggtcagaccc agcacacccg
    27481 gtagtgctga acattgaatt ccaaataggt tctggcagct tatcttcaat ccctgatacg
    27541 tcatcgggat gttgatgttc agcttgagat cgtagtgaag ggtttgctcg gcgagagtca
    27601 gctcttgctc ggtcacggag ccacagccgc cggttgccgc gcctaacagc gcagccgcgc
    27661 aagccaagga aaggaaacga cgctttgcgg aggataaagt ctcaattaga tattttctca
    27721 taaagagaaa tatatcatat cgagcccttc gccaaaagag gcagatagca tgactggatg
    27781 ctcagacgag tacccaggtt atgccgaagt gctgttttcg agaagattac agctcagctt
    27841 gaatcttcgg tgcggtcacg cgcagcaggt atcgaccttt gccgctgttg ccgctcggcg
    27901 caagcgccag cgcaacgaag tagtctttcg agatgacccg gataaggaga gtcatccgct
    27961 cggtgcgaat gctgatctcc tcgacactgc caccttcgat gatgtcggcg gctttcttga
    28021 cctgggccaa gatgaacgaa aactccatgc cgacggtctg gatgtccagc ttttgggcgt
    28081 ctttggcaaa gctctcgacg gcaattccgt caaagcccat cagcagtcca gcgaccgcac
    28141 catctgtctt ctctacaagc gtgcgcaagc tatctcggaa cataagtttc tttttaccgg
    28201 tttttgccgg caaaggtcaa ttgcttccgc ttgtgtcccc cgaaggttag cccgcacgca
    28261 agggatgtgg gcgatgcgcc gtgcatggta tggtgcgcgc ttcgcttagg ccgagccagg
    28321 ttatgtcgca cgcgagccga acgtctgcca aaacaagcga tctctcgatg acaatctcgc
    28381 ccgcagaggc ccagacgatc accgatccgc gcatcgcgac ccacctgatc gggccaagtg
    28441 gcgatccgct cgggccgggc ggcgatccgc tggcggcgac ggcacttgcc aacacgctgc
    28501 caagtccagg cccaagtgca tccaccggaa aaccgggtga atcgggacag ccagcgacga
    28561 cggtgcgggc atcgggggcg caaagaagtg gttttagttc atcgctgctc gactgtgaca
    28621 ttcccccgcc cgccgaagtc gaaggctacg agatcaaaaa accgctcgga cggggcgcg
//
LOCUS       DAORGN010000046        28434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_633_length_28434_cov_5.833962, whole genome shotgun sequence.
ACCESSION   DAORGN010000046 DAORGN010000000
VERSION     DAORGN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28434
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_28434_cov_5.833962"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKL17_07400"
     CDS             complement(<1..274)
                     /locus_tag="PKL17_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44590.1"
                     /translation="MSKPSREQIQRAVSYFEQAEAIWIGAGAGLSVAAGVDFSDEKSF
                     ARDYPGMLQYGVRRKLDMMGVWDLPQELTWGYYLPHVKETRFGEVRG"
     gene            complement(308..1168)
                     /locus_tag="PKL17_07405"
     CDS             complement(308..1168)
                     /locus_tag="PKL17_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HNK44591.1"
                     /translation="MLELSDYRDWIGLDKPFVRPTPLAASDVPAVVDTLCQTFVREPR
                     RTPFQVRKGLSPRELLYALLISRDPLPIPSAVMDLLDRLWALESVQRRTVTQTALPLG
                     VSVWKGDIAELSVCAIVNAANDALLGCFRPFHACIDNRIHAVAGPALRADCQQIMTRQ
                     GHPEPTGRAKITRAYHLPSRYVLHTVGPIVNLDASVVPDSQCTELRSCYLSCLDLAAQ
                     VPDIRSIALCCISTGAFSFPKSQAADIALSAVDDWLDRNPARFDRIVFNVFTETDHVL
                     YRDKLAFRSR"
     gene            complement(1256..2431)
                     /locus_tag="PKL17_07410"
     CDS             complement(1256..2431)
                     /locus_tag="PKL17_07410"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HNK44592.1"
                     /translation="MKRISYVALIALALSCDSTGSTGNDVAAPTCTDGQKNGTESDID
                     CGGSCGACVVGKHCALAADCTSGTCIAGSCMNPTCTDGVKNGGESDVDCGGSCGACAD
                     GKACANPADCMSSVCSAKTCTAPVCSDGVKNGSETDVDCGGGCPSCVAGLRCVQNSDC
                     ATGFVCDQTSSVCRNPASCQELLSRRPGTLTGTYLLLPAGVTTPFLANCEMTKDGGGW
                     TMVLKSNGDATLGYGSALWTDTNLLNPTDLSYSPGNAKFQGFLSVPGGELRGELDGYS
                     FKLKLAQSQTAQAVFQGPASVSSPFSVALGTGANWSVQPNCQTFGVNTPYAYQRTRFG
                     WTANQEADCNSNDTAIGLGLGATNTTAQDRGAGYACLSTNCSQGNVNTGGSGFLWVR"
     gene            complement(2635..4275)
                     /locus_tag="PKL17_07415"
     CDS             complement(2635..4275)
                     /locus_tag="PKL17_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNK44593.1"
                     /translation="MSLRAKQVVIIILGLVFLGSLLLVQYMEVLRKKQESGDRATAHV
                     VVPQSSQSCVKCHQEKTPNIVEHWKFSTHSQKGVGCLDCHKADRDDADAFIHEGAVIA
                     TIVTPKDCSGCHPREFDEFTHSHHSAAGNILASLDNYLAETVEGHRGSFNPHGPTVGR
                     SDINNVNGMASAASGCQQCHGAKVALKTKDGGRITLDDLKPDANGRPTNALVLANVLR
                     TADGKPMLHESTWPNTGIGRLNLDGSRGSCSACHSRHDFSPRRARQPENCGKCHLGPD
                     HPQEEIYQESKHGIAYKDLQSRMNLDAKTWILGQDYSAAPTCATCHMGGNLRNGGKVT
                     HDPGERISWTNRPPVSKLLDTDAKHRIVSEVDPAKRKELITDSWEQKRGRMKEVCGHC
                     HSPDYVTAFYVQYDELVILYNEKFGKPGQSIMDEMYKQGLISKTQFDEPVEWTWFYLW
                     HHEGRRARHGASMMAPDYTHWHGMYEVADRWYMHFIPEVREIIKKARETGKAPQADAA
                     QAVVDKIMAKPEHSWYQGGSDAQYDEIKKQMEQRYGSK"
     gene            complement(4294..5340)
                     /locus_tag="PKL17_07420"
     CDS             complement(4294..5340)
                     /locus_tag="PKL17_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNK44594.1"
                     /translation="MGTLNQFFKERVPVSSETMAELTNEPVPNHLKRWWFALGGTPAY
                     LFVIMIVTGILLACYYQPGPTTAYQSVRFITEEVAFGWYIRSLHKWSATFMVAGVILH
                     QMRVFFTGAYRRPRELNWMIGMGLLVSTLVGGFTGYSLVFEQLSYWGATVASNIADAV
                     PLVGSFAKRMLLAGDTYNSHTLSRLFVIHAALMPTLMVLLLVLHIAMVRLHGVTEFRF
                     PDDKSEQKYFNFIPDHLFTELIVGLLLMIVLSTLAVTLPAEMGKIADPLTTPEVIKPE
                     WYFYVSFRWLKLFPATVAVLSMGLIVFLFFVWPFVDGLIRRRWPGSEASLWIGAVSVI
                     TIVSLTVWEAVVAH"
     gene            complement(5327..5968)
                     /locus_tag="PKL17_07425"
     CDS             complement(5327..5968)
                     /locus_tag="PKL17_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNK44595.1"
                     /translation="MADKPEDDPQAQRDHHSEKDSDPSRRDLLSVALNAGVALSLGAS
                     YGMLGGYAISYLYPTQSEPKSWLYVSQVGTIPTGGQVKFIAPSGASVAVARIAEGADS
                     NCFIALSSTCPHMGCQVQWEANKNRFFCPCHNGVFNERGEGIDGPPKGQSLARYPIKV
                     ENGLLFIEVAVEALPQQKKQLASVNTRSGCLREPRRLPVLPSGGRGGDSDGHA"
     gene            6661..7425
                     /locus_tag="PKL17_07430"
     CDS             6661..7425
                     /locus_tag="PKL17_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNK44596.1"
                     /translation="MEALWQHLTASASNFTLYALFPTIVVNITWIVSGLFCLGLDSIP
                     ALRPYKIQHHVNGTAEFWKVVLHSLRNKLISEVPLTFASYPFFVWLGISKDLPLPTVT
                     TILGTLVFCLLVEDTWHYFAHRALHTRWAMDNIHSLHHHYMTPFGLASSYAHPLETLF
                     TGFGTVLPMLILRPHLFTLLIWVVVKQLQATAVHCGYMFPFRPSRYLPFLVSAGFHDR
                     HHRRFNRNFAPNFVFWDKLLGTADEKNLGPVAERAK"
     gene            7437..9230
                     /locus_tag="PKL17_07435"
     CDS             7437..9230
                     /locus_tag="PKL17_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK44597.1"
                     /translation="MQVLSLLDRAIGRAIGRDKPIDVPSGLRLPPLLSGGLPVLGHAS
                     DFVTGAIQLLFRAHREHGEICRLNVFHRNMVAMFGPDAHEAVFRAPDTVLNPSEAYKI
                     MTPVFGKGIVYDAPPDKQAEQFKMLLPALKDKRMRTYGEAVVQETEESTRGWGDDGTL
                     DFVEFCRVLTNFTSSRCLLGREFREGMNDEFAAVYHDLEMGVTPLAYLNAHLPIPSFI
                     RRDRARVRLVEMISKITEARRREQRVGEDFLQTLMDAKYQDGRSLTEDEITGLLLAGM
                     FAGHHTSSVTTAWAMIDLLRNPASLKRAIDEVDRVFASGRAVNHAALRELSFLENVVK
                     ETLRLHPPLFMLVRVAQTDFTFKDFFIPKGTWILISPTVSHRIPEVFPHPETFDPDRF
                     APPREEDKRDFAYIAFGGGRHKCLGNAFAILQIKAILALLLGQYEFELAGDPIDSDFH
                     GLVVGPKVPCRIRYSRRANPSVTIAGATALAKLADEYGQAAQQGGEAGKGGCPVAHPS
                     AAQEAAPASKPETEEKADAAAQPKKLTVMLDKDLCQGHAVCAGEAPELFYVAGDGKVA
                     LKQEDIPPEQYAKLRAAAKFCPTRAIRIKEQ"
     gene            9363..10142
                     /locus_tag="PKL17_07440"
     CDS             9363..10142
                     /locus_tag="PKL17_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNK44598.1"
                     /translation="MTLGYLTAGLLVGFLAGLTGVGGGSLMTPLLVFLFRFKPVVAVG
                     TDLLFAAITKTGGVLVHHGKHKSVDWQIVGLLAAGSLPGSIGTLLVLQNYARGAGLNR
                     IVTSSLGVALILVALAQLLRSTLVQFGKNQDARIPSPLKKLRTPLTIAVGLLLGVLVT
                     LSSVGAGALGTVAVLALYPRLPTLKVVGTDLAFAIPLTAVAGLGHWRLGHVDFSLLGS
                     LLLGSLPGIWLGSLLSAKIPERILRPLLAILLLLIGLKFVI"
     gene            complement(10152..11858)
                     /locus_tag="PKL17_07445"
     CDS             complement(10152..11858)
                     /locus_tag="PKL17_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNK44599.1"
                     /translation="MTQLDWLILIGTLAVIVSVGLWKGRSVRTAEQYLRAGRDMKWYT
                     IGLSIMATQASAITFLSMPGQAYEDGLGFVQFYFGLPVAMVILSAAIVPIFSRLRVAT
                     AYEYLEHRFDRRTRMLTAFLFLLQRGLGTGISIYAPAIILSTVLGWPLHLTNLLIGVL
                     VIVYTVSGGTRVVSQTQTYQMIVMLSGVLLAFLFILSALPKEVSLPDALYIAGSLGKL
                     HAVDFSLRLDTRYTFWSGMTGGLFVALAYFGTDQTQVQRYLTGGSLVESRLGLLLNGI
                     VKIPMQFVILLVGVLVLVFYQFQRPPLFFHATELHRAEQSVYSAELRSVEDSYSQVFS
                     RKQKLIDDLLLARRSGNVEAMQSAKKSLQSADSEAKQLRQAAKGIVRKANPRAETKDA
                     DYIFITFVKDHFPSGLYGLFLAVIFCAAMSATASALNALGATTVVDFYKTSLRPHESD
                     RHYFLAAKGFTILWGLLAMLFATFASLVDNLIQAVNILGSIFYGPTLGVFLVGFFLPR
                     VGAKSVFVSLVAAQALVLLVFAFSSIGFLWYNVIGCGAVLILSILGMRFETAPKPTVT
                     TS"
     gene            complement(11855..11992)
                     /locus_tag="PKL17_07450"
     CDS             complement(11855..11992)
                     /locus_tag="PKL17_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44600.1"
                     /translation="MNPSEHQTPCDPPPILKSWGRLYVLTLGMLFLSVLLLFVLGKVF
                     S"
     gene            complement(11982..14450)
                     /locus_tag="PKL17_07455"
     CDS             complement(11982..14450)
                     /locus_tag="PKL17_07455"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNK44601.1"
                     /translation="MVSSAHSPPKQPSGAELLRAIDRLSVVGNVLYVAAHPDDENTRL
                     LAYLSSDLQVRTAYLSLTRGDGGQNLIGPEQGPLLGLIRTQELLAARRVDSAEQFFTR
                     ARDFGYSKTPAETLSIWDRNAVLEDTVWVIRKFRPDVIITRFPTQGLDTHGHHTASAM
                     LAEEAFAAAADPNFAKQQLAQVSVWQARRLLWNRSPWRSLAGEDLSKFLKLDVGSYNP
                     VLGVSYGELASESRSMHKSQGFGSAKQRGASPEYFLPLLPKSESKLPASLFEGIDLSW
                     NRVAGGGPLALLLAKISKSFDLRRPEASIPQLLQARRELLRLPDSPWKQPKLREIEDI
                     IVACAGLYAEASASDHAITEGSDLSVSLQVINRSTAPLRLREVRLSTGEMLSVEQNLA
                     SGELWQKEQTIRIPAQTPIGNPYWLTQQPLPGLYPVRDPLRIGMPEDPPVAQAAFVIE
                     WSGPQGEKETLTVDRAVLHKWTDPVAGERTRSIEVWPQVMVNVDGKVMMFADGEKKPL
                     RVRLKAGQADVKGTVRIEVPSGFSVEPSAIPFQLDKKGAEEEVLFQVRPKANPQATPI
                     VGTLRVIAETNGQSFDRGIVRIEHPHIPIQTVFPEASVSLCRFDLKKTRSRIGYIAGA
                     GDDVAAALRQVGFDVTQLSEEQILQKPLAPYGAIVVGVRAFNVNPRLPFYYKKLMDYV
                     QAGGNLVVQYSTSNRLGKLQVPIGPHPFEISTDRVTDENAPVTFALPNHPILQRPNKI
                     TAADFSGWVQERGLYFADKIDEKYETPLSLRDPGEPERRGSLLFVRHGKGSFVYTGLA
                     FFRQLPAGVPGAFRLFANLISHEP"
     gene            14668..16332
                     /gene="bshC"
                     /locus_tag="PKL17_07460"
     CDS             14668..16332
                     /gene="bshC"
                     /locus_tag="PKL17_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNK44602.1"
                     /translation="MLASFGAAYLKGDPLAAGFLDGDFRDPLSLRAIVAQRTSQRISV
                     ELAALLTTQQNPLGQSVARERHMQQLCAAQGAGVVVTGQQVGLFLGPLYTVYKAATAV
                     AAARTLSAQTGQPIVPLFWLQTEDHDYEEIRSCTIPSPNKGEPPLVLSLPPSDPAEQR
                     MSVAYRQLGPEVDALLSELESWLHPLPHAADCLSLLRRHYAIGRSLAQAFSGLLCELF
                     ADEGLLCLDPRQPGQDTLPKLAAPLYRNILTSHETLGSALLARQRALSDRGFACQIPV
                     RPHSSLLFFHPDGERSPRHRLQQHQQTGPDGHASLLQWSIPQDGRRYSQSDLLARMDS
                     DPLSFGTSALVRPLVQDTILPTVAYVGGPAELSYFAQVTPLYHALGIVQPVIMPRARF
                     RLIDESTRTALAKLALRACDVEAPKDDIMLRLAQGKPADVPSPQAVEERLLAQLLSPL
                     SEIDSLDPALQDAVHTARRVMEKTAKKISLRYAQRLHEKDTVNSERIDRLQAAIFPSS
                     TPQERLFSLPFYLAKYGLFGWKQRLFESLAARSVFSADQSVRDIFL"
     gene            16352..17560
                     /gene="bshA"
                     /locus_tag="PKL17_07465"
     CDS             16352..17560
                     /gene="bshA"
                     /locus_tag="PKL17_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNK44603.1"
                     /translation="MPLRIALICFPTVGGSGKIATDLGRTLARRGHSVHLLSYDRPGF
                     IFAPEANLHVEKIAIPDYPLLHLSAYAMSLASQLAELSQHVGFDLWHLHYAIPHMMSA
                     YLAMQLLRDRNAPIPKVVTTLHGTDVTLVGSDPALVPMHRFLLSQCDALTTPSAYLRD
                     AAHAQIGIPQTSPIQVIPNFVDTEFFCPLRPSPSSSERCALLSQLLGKTISEEESATT
                     AVLLHSSNFRPVKRIDDVIQLFVRVTQLLPEGRRGVLFLVGDGPEKPRMEALAESMGV
                     AAAVRFLGIQHDFRTLLQHSDVFLLPSTIEGFGLSALEALSCGVPVVASRAGGLVEVV
                     EDGKTGFLCPVGDVAAMADAVLRILSVPNLRSALSQNARRQVEERWQSDQVIDQYESF
                     YMHILRHYPL"
     gene            17628..18173
                     /gene="def"
                     /locus_tag="PKL17_07470"
     CDS             17628..18173
                     /gene="def"
                     /locus_tag="PKL17_07470"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNK44604.1"
                     /translation="MSLLKIVRFPSPFLRQPTQEVKTIDKEIRTLVSDMMETMCDVNG
                     AGLAAIQVGSLHRIFIIEALAAGGERTDPPKVFINPVIEQLSPDTDVRDEGCLSFPGI
                     FISVKRSLKAKVSALDLDGKPFSVDAEEFFARAIQHEYDHLVNKLLYDHAGPIKKQMI
                     KRKLDKMTDDEAMDLLRQYGE"
     gene            18267..>19110
                     /locus_tag="PKL17_07475"
     CDS             18267..>19110
                     /locus_tag="PKL17_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44605.1"
                     /translation="MSRTRSAFGFDIAAVTFASFLLGATAQAQTAQKVDAADLIAAQK
                     AGGPAAAAYNGKLLEVFNLKINGFFPVNDALMRRNPALDLRTSKYFDYIACESTLANS
                     PELAKLATGQQLVVTGVAQVGTYSLRLSGCTIVKLLDPVLPPSVSMADTGGKVTAADV
                     VTDYLRNSVAATLKYKGKVIAISGKLSDTSGGKLKLRGTEFNSVTCSVPGPVATFAAS
                     LPSGSSVVVKGTVSEMDTMDLQVKDCSLVDPVQPPPAAAPVAAQAAPVAAPTAPVAAP
                     TAPVA"
     assembly_gap    19111..19210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19211..19534
                     /locus_tag="PKL17_07480"
     CDS             <19211..19534
                     /locus_tag="PKL17_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44606.1"
                     /translation="AAVKFKAADLKSYYPTAVHAGGNWTMTGVAAGIPVQVRMDEKDG
                     SPTRGHFFITIAGGTERPMNKTELRMFYDGMLARAKAPGAAPFEKEAARALAQALGIK
                     PPKLP"
     gene            complement(19553..21181)
                     /gene="pruA"
                     /locus_tag="PKL17_07485"
     CDS             complement(19553..21181)
                     /gene="pruA"
                     /locus_tag="PKL17_07485"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNK44607.1"
                     /translation="MQDTIARYGEPKNEPVFGYLPGSAERNALKAELAKMGGASVDVP
                     LYIGGERVRTGKTRNIVTPHSHRHSLGVFHQGGSSHVEQAIAQALAAQADWAALPWQQ
                     RAAVFLKAADLLSGPWRMRLNAATMLGQSKTCYQAEIDAACELADFFRFNVYFYRRLL
                     EADQPLSPTPTWNQLDLRPLEGFVFAVTPFNFTSIAGNLPTAPALLGNVVLWKPAATS
                     MLSAYYIMELLLEAGLPPGVINLVAGPAAEIGAVALSHPSLAGIHFTGSTGVFQQMWG
                     TVGNNIQRYHTYPRLVGETGGKDFILAHESADEDALVAAIVRGGFEYQGQKCSAASRI
                     YVPRPLWGKIRDKLCAEIESIKVGDPTDFRNFMGAVIDAGAYSSITSYIRAASSIPGA
                     RVLCGGESDDSLGYFIRPTLVEAEDPRHKLMCEEIFGPVVTLHVYDDWQEALRLVDTS
                     SPYALTGAVFARSRTVIDQALLRLRHAAGNFYINDKPTGAVVGQQPFGGARGSGTNDK
                     AGSAMNLLRWTSLRAIKENFVPPRDYRYGFLAAE"
     gene            complement(21211..22668)
                     /locus_tag="PKL17_07490"
     CDS             complement(21211..22668)
                     /locus_tag="PKL17_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44608.1"
                     /translation="MFDWLLRHVLLRGLSRATIDACLAHPEVRALLRFRFAYLFAFHA
                     PVLVPLLDALSGPDASGQGGDPSLSVLSLGLASLTMVLADVPTGFYADRHGAKASLSL
                     GLWATNGIMVAFFGMALLRALWPTQFVALSLGPWAVLFLEGAIGVSLALLSGADTVLF
                     LQVARRSQIPGLLHPSFEGVGSSIRYLGTMVAVLLGALFYDLCAWLVPALHLRTAVQS
                     GLFLLTSAAELVALAELAKLRDDHTPLHRPGLRELFRGFSVVLGYPRFFAELWLLSMA
                     AAVALFAVYLVQSPLSRLSTALATQHPLLWPLYTLVAVLGYWACSVGSQRFARHRGEA
                     LIVLPRRSLSLTLGSWLLLLLLPLGFYATENLSIQPVVVLTLCIVLCLLFNFVRGFIE
                     PYSAATLIRFTEEQGAKVPASLVSGFNSLKRGAHFACSALFFVLQRRFVTADPDARLA
                     RSLAFVALGFALVCLPVLPRLLARPSSLPGGQKTA"
     gene            complement(22675..23469)
                     /locus_tag="PKL17_07495"
     CDS             complement(22675..23469)
                     /locus_tag="PKL17_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44609.1"
                     /translation="MKRYHLFEFCDLPWLPPFFRSLTPQFLEAAFRLLGSYSVAVPML
                     AKALRATGSVELLDLGSGGAGPLPQLLDGLAKDHGLMVRATMSDKFPNHAALSAAVRR
                     DPERLSFLPDSVDATAVPAHAAGLRTMFQLLHHFPPETARAILQDAVRCQRGIAIFEV
                     TQRRLLGVVLALLIPFVVLLVTPLVRPLRLWRLLLTYVVPIAPLVIAWDALVSTLRSY
                     TEDELRAMTDSLDGPAYVWQIGHGRKLLSEVTWLIGYPATGETGKD"
     gene            complement(23466..24797)
                     /locus_tag="PKL17_07500"
     CDS             complement(23466..24797)
                     /locus_tag="PKL17_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HNK44610.1"
                     /translation="MERALADMERAFYLLDRGTRFNGVQVVTLRGAVDVALLRQALLR
                     LQARHPLLRVRLRGDDKKLFLTDEDHGPLPLTVQTRRSDSDWIALAEDELNRPFDREG
                     DHLTRLQLLLGDDRSDLILSHHHVTADALSIIFAIRDLLTDLDTLRRGDILPPPESLP
                     LRPPMPALLPPSARGLRLLPAMHHFFFKHVVGKPLRRAKTLPLSQPMPAAQRRNRVVH
                     RALVGETLTALKARVKKEQTTVHGALAAALLLSTSQEVFAERIAQNRATKAGCFSAVN
                     LRGDLTPSVGEEMGLYISQVTTFHQVVPPPSLWNLARELRQQLTRTLAQGEQYLTMPL
                     IGLFIPRFGDVAARFVKNFDAASPALTGVTNLQKLPIPLEYGDLQIEDYQITVGLSVV
                     GQLLLAVTTVGDRLNLNLVFVDPLVSPDRATRIADGLLAQLDAALGVSEAA"
     gene            complement(24830..26623)
                     /locus_tag="PKL17_07505"
     CDS             complement(24830..26623)
                     /locus_tag="PKL17_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44611.1"
                     /translation="MPQKPAAAPTEMPAADFEEDTLLPPPARSLLAPEHEPPDEALLE
                     ADRAMLPARLSQEKSSPELSSEQSAIPEHVIDVPTMQGFLATRPIDGISDPLAAQSGD
                     AIARVMAELPVDRTQQMPLFLPSRLRGDETPRQRLDVGPIPPSAMTQELVPIDPAATV
                     ELPQVPRSLTRRFQAVLPNATAQMLPVSPHETAKLDPVPSSQTMRFPTLPTSQRVPAR
                     SVPITAPPPSYSAEIPSPSDPDKGESTAEIARAALASAATMEMPMVPSDDRDAESVLS
                     VSTRSLPKISLPPPSEDTLPPLPLLPPSDTLWGRLSYAVSGLRSLMRRRKQLRCLLRD
                     LGERLSAYDRALVQLGELAYAENIELLRDPNNSDGAVSTELPASKDAALLAVQVVADR
                     LWAKAQKEQRSLARKEAVLSSELRVLRHRRDELYQRLLLLAGDPHASEASGDERFSVG
                     NELTGIESDRQKVAARLGEVRALWRLVRRKNHHLKSRRAELVYALSLEYAESVQKSPH
                     LLVLGTLCASVRPLPAFGKDKRARFDSLYAVLDALRSGIAKVETRLALLDGDRRAADQ
                     KAWKSALLLLGGLALIGGGIVGGLLYYLLLP"
     gene            26854..27114
                     /locus_tag="PKL17_07510"
     CDS             26854..27114
                     /locus_tag="PKL17_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK44612.1"
                     /translation="MTTARRPRSEGRLAKYLRRCERDFILKALRMHRGHNSQTAAWLG
                     VSRRALYDKMRDYGLDGEASAMRTEAGIMGPRKVDLDGGDED"
     gene            27264..28049
                     /locus_tag="PKL17_07515"
     CDS             27264..28049
                     /locus_tag="PKL17_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNK44613.1"
                     /translation="MSETPPTDYELYLRVPELLRLQKQPSELSCPDELLFQVVHQAAE
                     LWMKLADFEMRLLGPLISSERLAEASKTLRRVHAVLVQLAQSLETLYTLTPSDYMRIR
                     EVLGRGSGQESPGFKRLLAVPAEVYPHFEQLLAKRKLTLIELYREPSRHADVFQVAEA
                     LIDFDLRLQEWRNRHILMVYRTIGVGTPSLKGKHSEMLERGLRQQFFPKLWEVRDELY
                     GEWSAKNPSGADYSLTGTVKPEPAPVETPLPARAPKPPRRKTK"
     gene            28057..>28434
                     /locus_tag="PKL17_07520"
     CDS             28057..>28434
                     /locus_tag="PKL17_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNK44614.1"
                     /translation="MHEPTSGSDNEQTLLSLRSEFPALAQSVYMISHSLGAMPRATTE
                     ALSEFTSLWVQKSIVAWEDWLRQAQLAASRIGHIIGAAPGTVQMATNVSQVQATIASC
                     LDFAKPRNRVVYTDMNFPSVSYVW"
BASE COUNT         6054 a         8709 c         8067 g         5504 t
ORIGIN      
        1 ctccacggac ttcgccaaag cgcgtctctt tgacatgcgg caagtaatat ccccaggtca
       61 gttcctgcgg caaatcccag actcccatca tgtcgagctt gcgacgcacg ccgtactgaa
      121 gcattccagg gtagtcccgc gcaaacgact tctcgtcgga aaaatcgact ccggcagcca
      181 ccgaaagacc ggcgccggcc ccaatccaga tcgcctctgc ttgttcaaaa taggacaccg
      241 cacgctggat ctgttcccgc gatggcttgc tcatttatga ctccttagct gattcctaac
      301 gtgtttccta acgtgaacgg aatgcgagct tgtcgcgata cagtacgtgg tcggtttccg
      361 tgaacacgtt gaacacaatc cgatcgaacc gagccggatt cctatccagc caatcatcga
      421 cggcagacag cgcaatgtcc gccgcttggg actttggaaa agaaaacgcc ccggtggaaa
      481 tgcagcacag cgcaatactg cgaatgtcag gcacctgcgc agccagatcc agacacgaca
      541 gatagcagct ccgcagctct gtacactgcg aatccggaac cacagacgca tcgagattca
      601 caatcggccc caccgtatgc aagacgtatc gacttggcag atgataggcg cgcgtgattt
      661 tcgcccttcc cgtcggttcc ggatgacctt gccgcgtcat gatctgctga cagtcggccc
      721 gcagcgcagg accagctacc gcatgaatcc ggttgtcgat gcaggcatga aacggcctga
      781 aacagccgag cagcgcatcg ttggccgcat tcacaatcgc acagactgac agttcggcga
      841 tgtctccctt ccacacagag accccgagcg gaagcgctgt ctgcgtcact gtccgccgct
      901 gcacggattc caaggcccac agtcgatcca gcagatccat cacagcggaa ggtatcggca
      961 acggatcacg cgaaatcagc agcgcataca gaagctcacg cggagaaagt cccttgcgaa
     1021 cctgaaaggg agtccgccgc ggctcgcgca caaaggtctg gcagagtgta tccactaccg
     1081 ccggtacgtc gctcgcagcc agcggcgtgg gacgcacaaa cggcttatcg agaccgatcc
     1141 agtcgcggta atcggacagt tccagcatgc ttccaatgtg aacatgccgg tctgtccgaa
     1201 caatagtgcc gaaagcgaac agtttgttcg gttctattga acaatcaagg gacagttagc
     1261 gcacccacag aaagccgctg ccgccggtgt tgacgtttcc ttggctacag tttgtcgaca
     1321 gacaggcata gcccgcgccg cgatcctgcg ccgtcgtgtt cgtcgcccca agcccaagtc
     1381 cgatggccgt gtcattcgag ttgcagtcgg cttcctggtt cgcagtccag ccaaaccgcg
     1441 tccgctgata ggcataaggc gtgttgacgc cgaatgtctg acagtttggc tgcaccgacc
     1501 agtttgcgcc cgttcccagc gccaccgaaa acggactcga caccgatgcg gggccctgaa
     1561 agaccgcctg cgccgtctgg ctctgcgcaa gcttcagctt gaagctgtac ccgtctagct
     1621 cgccgcgcag ctcgccacca ggaaccgaaa ggaatccttg gaatttggca ttgccgggcg
     1681 aatacgaaag gtcggtcggg tttagcaggt tggtgtctgt ccacagcgcc gatccatatc
     1741 ccagcgtggc gtctccgttt gacttgagca ccatcgtcca gcccccgcca tctttggtca
     1801 tttcgcagtt ggcgagaaac ggcgtcgtca ccccggcggg caacagcaga tacgttccgg
     1861 tcagggtgcc tggccgacgg gacagaagct cctgacagct tgccggattg cggcatacgg
     1921 agctggtttg atcgcagaca aaacccgttg cgcagtcact gttttggacg cagcgcagac
     1981 cggcgacaca gctcggacag ccaccgccgc agtccacatc ggtttccgat ccatttttca
     2041 cgccatccga acagactggt gcggtgcagg ttttcgccga acacaccgat gacatgcagt
     2101 ccgcagggtt tgcacacgct ttgccatctg cacaagctcc gcatgatccg ccacagtcca
     2161 cgtcggattc accgccgttt ttcaccccgt ccgtacaggt tggattcata cagcttccgg
     2221 cgatgcaggt gccgcttgtg cagtccgcag ccagggcaca gtgcttgccg accacacacg
     2281 cgccgcagct tccgccacag tcaatgtccg actccgttcc gttcttttgt ccgtcggtac
     2341 aggtgggcgc tgccacatcg tttccggtcg atccagtact gtcacaagac agcgcgagcg
     2401 caatcaaggc cacataggat atccgcttca tagacacctc ccatgctgat cataagggag
     2461 atccaatcag cagcacgtac cgtcgcagaa cgcggcgttt ggacagtgaa ttttgcccca
     2521 agtggaaagc acaaagcgcc gcgctgcggc ctgggaagga aacgaaaggg agggagagga
     2581 gagaactaca gagcgtcgag cgtcaagcgg cggactgcgc gcaaaagcgg gcggctactt
     2641 cgatccgtag cgctgctcca tctgcttttt gatctcatcg tactgcgcgt cgctgccgcc
     2701 ctgataccaa gagtgttcgg gcttggccat gatcttgtcg acgacagcct gcgccgcatc
     2761 ggcttgtggg gctttgccgg tctcacgcgc cttcttgatg atctcgcgca cttccggaat
     2821 gaagtgcatg taccagcgat ccgcgacttc atacatgccg tgccagtgcg tgtagtcggg
     2881 agccatcatg ctggcaccgt gacgcgcgcg gcgtccttcg tggtgccaca gatagaacca
     2941 cgtccactcg accggctcgt cgaactgcgt cttgctgatg agcccctgct tgtacatctc
     3001 atccatgatc gactggccgg gcttgccgaa cttttcgtta tacagaatca ccagctcgtc
     3061 gtactggacg tagaacgcgg tgacgtaatc gggcgagtgg cagtgaccgc agacttcttt
     3121 catccgtccg cgcttctgtt cccacgaatc ggtgatcagc tctttgcgtt tggccggatc
     3181 cacttcggac acaatgcggt gtttggcatc ggtatccaaa agcttcgaca ccggcggacg
     3241 gttggtccag ctgatccgct cacccggatc gtgcgtcact ttgccgccat tgcgcagatt
     3301 gccgcccatg tggcacgtcg cgcacgtcgg tgccgcgctg tagtcctgtc ccaaaatcca
     3361 ggtcttggca tccaggttca tgcgcgactg gagatccttg taagcaattc cgtgcttgct
     3421 ctcctgatag atttcttctt gcggatggtc cggtccaagg tggcacttgc cgcagttttc
     3481 cggctgccgg gcgcgacgcg gactgaagtc gtggcgcgag tgacaggcac tgcatgaacc
     3541 acgcgaacca tcgaggttca gccgaccaat tccggtgttc ggccaggtcg attcatgcag
     3601 catcggcttg ccatcggcgg tgcgcagcac attggccagc accagcgcgt tggtcggtcg
     3661 accgtttgca tccggcttca gatcatcgag agtaatccgt ccgccgtctt tggtcttcag
     3721 cgcgactttc gcaccgtggc actgctgaca gccgctggcc gcggaagcca ttccgtttac
     3781 gttatttatg tcgctgcgac cgaccgtggg cccgtgcgga ttgaacgatc cacgatggcc
     3841 ttcgaccgtt tcggccaggt agttgtcgag cgacgccaag atgttgcccg ccgcagagtg
     3901 atgagagtgc gtaaactcat caaactcacg cgggtgacag cctgaacagt ctttcggcgt
     3961 tacgatcgtt gcgatgactg cgccttcatg gatgaacgca tcggcgtcat cgcgatcggc
     4021 tttgtgacag tccagacagc cgacaccctt ttgggagtgg gtactgaact tccagtgctc
     4081 gacgatgttg ggggtctttt cctggtggca cttcacacag gactgcgagc tttgcggaac
     4141 caccacatgt gcggttgcgc gatcgccgct ttcctgcttt ttgcgcagca cttccatgta
     4201 ctgcacaagc agaagagaac ccaagaacac aaggcccagg atgatgatga caacttgttt
     4261 tgcgcggagt gacatgatgt gcgcgactcc tttttagtgc gcgacgacgg cttcccacac
     4321 cgtcagcgat acgatggtga tgaccgaaac cgcaccaatc cacaggctgg cttcgctgcc
     4381 cggccagcgt cgacgaatca gtccatcgac aaacggccag acgaaaaaca ggaacacgat
     4441 aagacccatg ctcagcaccg ccacggtagc gggaaagagc ttgagccagc gaaacgaaac
     4501 gtaaaaatac cactctggct tgatcacttc cggcgtggtc agcgggtccg cgatcttgcc
     4561 catttcagcc ggcagcgtta ccgccagcgt cgacagaacg atcatcagca acagtcccac
     4621 gatcagctcg gtaaacaggt gatcgggaat gaagttgaag tatttctgtt cgctcttgtc
     4681 gtccggaaag cgaaattccg tcacgccatg cagccgaacc atcgcgatgt gaagcaccaa
     4741 aaggagcacc atcagcgtcg gcatcagcgc agcgtgaatc acgaaaagcc gcgacagcgt
     4801 atgggaattg taggtatccc ccgcaagcag catgcgcttg gcaaaactgc ctaccagcgg
     4861 caccgcatct gcgatgttgg aagcgactgt ggccccccaa taggagagct gctcgaacac
     4921 cagcgaatag ccggtaaaac cgccaaccag cgtcgaaacg agaagaccca ttccgatcat
     4981 ccagttcagc tcacgcggac gccgatacgc cccggtgaag aacacccgca tctggtgcag
     5041 gatcacaccc gcgaccatga acgtcgcgct ccacttgtgc agcgatcgga tgtaccaacc
     5101 gaacgcgact tcttcggtga taaagcgcac cgactgatac gccgtcgtcg gtcctggctg
     5161 atagtaacag gccagcaaaa tgcccgtcac gatcatgatc acaaacaggt atgccggcgt
     5221 tccaccgagc gcaaaccacc agcgcttgag gtgattggga accggctcgt tggtcagctc
     5281 tgccatcgtc tcggagctga ccggaacgcg ctctttgaag aactggttaa gcgtgcccat
     5341 cggaatcccc tccccggccc ccggatggaa gtaccggcag tcgcctgggc tcacgcagac
     5401 agcccgatcg ggtatttaca cttgcaagct gcttcttttg ctgcggcagt gcttcgacgg
     5461 cgacttcgat aaacagaaga ccgttttcga ctttgatggg atagcgcgcc aggctctgtc
     5521 ctttgggggg accgtcgatg ccttcgccgc gttcgttgaa tacgccgttg tgacacggac
     5581 agaagaagcg gtttttgttc gcttcccact gcacctgaca gcccatgtga gggcacgtac
     5641 tggacagcgc gatgaagcag tttgaatcgg cgccctctgc gatgcgtgcg acggcaacgc
     5701 tggcaccact cggtgcgatg aacttcactt gtccgccggt tggaatcgtc ccaacctggc
     5761 tcacatacag ccaggatttg ggctcgctct gcgtcggata cagataactt atcgcatacc
     5821 caccgagcat gccgtagctt gcgcccaaag acagcgcgac gccggcattg agtgcaacgc
     5881 tcagcagatc acgacgcgaa ggatcactgt ccttttcgct gtgatggtct cgctgtgctt
     5941 gcggatcgtc ctcgggtttg tccgccatgg tgtttcctcg acgaaagtaa gttgccctac
     6001 tgttcgagga caccatcaca atattgcgga taataaatac ggcttttttt ccgcggccaa
     6061 aaccctgcga tcttatgaag agttttccag gctgcaatcc gacgatgccg cgcgcgccca
     6121 accgaccagc aaacgcgctg gaagcacaca cagcgacagg caagcgtcgc acagcggtca
     6181 cagtgccgcc agtcctcacg ctccgtgatc gctccgctga atcaccgatg cggacagaat
     6241 tgcagcgata caacgccgca caccgcgctg gtcaaaagcg ccgcagtcgt tgacagagcc
     6301 gagcgcgtag cgcccctggc ccattacatg gccgcgaagg agcgcagcag aaacgaaagc
     6361 tgacgtttgg ggaggatctc gcagcgcaag atcggcaagc atggaagaca ggatcgatcg
     6421 ctcgatcgct cgcttgtgga acacgctgaa atggggcgat ttcgcaccca atcttccggt
     6481 agttcgcgca tccgcaccgc agcagatgga aacaccgtgc ggaactgcac agcgacgaaa
     6541 aaacacagct tgcaggtgcc gcagcgtttc cattttggac agcaaatcct cgtacacaaa
     6601 atccaagtgc ttctgctata agaccgccac tttctagcct acgcacggag cctgctgacg
     6661 atggaagcgc tttggcaaca cctgactgct tcggcctcga actttacgct gtacgcgctg
     6721 ttcccgacga tcgtcgtcaa catcacctgg atcgtgagcg gtctgttctg tttgggactg
     6781 gattcgattc cggcgctgcg tccatacaag atccaacatc acgtaaatgg aaccgccgag
     6841 ttctggaaag tggtgctgca ttcgctgcgc aacaagctga tttcggaagt gccgctgacg
     6901 tttgcttcct atccgttctt tgtctggctg ggaatcagca aagacctgcc acttccgacg
     6961 gtgacgacca ttttgggaac gctggtattc tgccttctgg tagaagacac ctggcactac
     7021 tttgcgcacc gcgcactgca cacgcgctgg gcgatggaca acatccattc cctgcatcat
     7081 cactacatga ctccgtttgg tttggcatct agctatgccc atcctctgga gactctgttt
     7141 actggtttcg gaaccgtctt gccgatgctg attctgcgtc cgcatctgtt tacgctgctc
     7201 atctgggtgg tggtcaagca gctacaagcg acggcagttc actgcggtta catgtttccg
     7261 ttccgcccca gtcgttatct tcctttcctg gtcagcgccg gattccatga caggcatcac
     7321 cgacgcttta accgcaactt cgcgcccaac tttgtctttt gggacaagct gcttgggacc
     7381 gcggatgaaa aaaacctcgg ccctgtggca gagcgcgcga aatagtgagg ccccacatgc
     7441 aagtactcag ccttttggat cgtgcgattg gtcgcgcgat tggacgtgac aaaccgatcg
     7501 atgtgccaag cggtcttcgg ctcccaccgc ttctgtccgg aggtcttccg gttctgggtc
     7561 acgcttccga tttcgtgact ggcgcgatcc agcttctgtt ccgtgcccac cgtgaacacg
     7621 gggaaatctg tcgactgaat gtcttccatc gcaacatggt ggcgatgttt ggtcccgatg
     7681 cccatgaagc ggtcttccgc gcccccgata cggtgttaaa tccgtcggaa gcgtacaaga
     7741 tcatgacgcc ggtgttcgga aaaggaatcg tctatgacgc gcctcccgac aagcaagcag
     7801 agcagtttaa aatgctgctg ccggcgctca aagacaaacg gatgcggaca tacggtgaag
     7861 ccgtggtcca agaaaccgaa gagagcacgc gcggctgggg cgatgacgga acgctcgatt
     7921 ttgtcgagtt ctgtcgcgtc ctgaccaact tcacatccag tcgctgtctg ctcggtcggg
     7981 aattccgcga aggcatgaat gatgagtttg ccgcggtcta tcacgatctg gaaatgggag
     8041 tgactccgct tgcgtatctg aacgcgcacc tgccgattcc gtcattcatt cggcgcgatc
     8101 gcgcgcgcgt tcgtttggtc gagatgatca gcaagatcac cgaagcccgg cgacgcgagc
     8161 agcgggtggg agaagacttc ctgcaaacgc tgatggatgc gaagtaccag gacggacgca
     8221 gcctgaccga agacgaaatc accgggctac tgctcgccgg aatgttcgcg ggacatcaca
     8281 caagttcggt cacaaccgcc tgggcgatga tcgatctgtt gcgcaatcct gcgtcgctca
     8341 agcgcgccat tgatgaagtc gatcgggtgt tcgcaagcgg tcgcgcggtc aatcacgcag
     8401 cactgcgcga gctttcgttt ttggaaaacg tggtcaaaga aacgctgcgt ctgcatccac
     8461 cgctgttcat gctggtgcgg gtggcgcaga ccgacttcac gttcaaagac ttcttcattc
     8521 ccaaaggcac ctggattcta atttctccga cggtgtcgca ccggattccg gaagtctttc
     8581 cgcaccccga aaccttcgat ccagatcgct ttgcgccgcc acgcgaagaa gacaagcgcg
     8641 actttgcata catcgcgttt ggaggcggtc gccacaagtg tctgggcaat gcgttcgcga
     8701 tccttcagat caaagcgatc ctggcgctgc tgctgggtca atatgagttt gagctggccg
     8761 gcgatccgat cgactcggac ttccatgggc ttgtggtcgg tccgaaagtg ccctgccgaa
     8821 ttcgctattc acggcgcgca aatccgtcgg tgacgatcgc cggagcgacc gcgctggcca
     8881 agctcgccga tgaatacgga caagccgcgc agcaaggtgg cgaagccgga aaaggtggct
     8941 gcccggttgc gcatcccagc gccgcacaag aagccgcgcc cgcatcgaaa cctgaaacgg
     9001 aagaaaaagc agatgctgcg gcccagccca aaaaactcac ggtgatgctc gacaaggatc
     9061 tgtgccaagg acacgccgtc tgcgcagggg aagccccaga gctgttctat gtggcgggtg
     9121 atggcaaggt cgcgctcaag caagaggaca ttccgcccga gcagtacgca aagctccgtg
     9181 ctgctgccaa gttctgtccg acccgcgcga ttcgcatcaa agagcaatag gaatccacat
     9241 ccgattccgc agtcgccaca gcgtcacttc acagccgcgc gcgggggact gcgtgtttgc
     9301 tgggtttctt tcgcaagccg cacgatctaa gctgcgcccg ttgtgcgaag gagtcgactc
     9361 cgatgacgct gggctatctg actgccggac tgctggttgg ttttttggct gggctcacgg
     9421 gcgtcggtgg cggttcgctg atgacaccgc ttttggtgtt cctgttccgt ttcaagcccg
     9481 tggtcgcggt cggtacggat ctgctgtttg cggcaattac caaaacggga ggagtgctgg
     9541 tgcatcacgg caaacacaag tcggtcgact ggcagatcgt cggtctgctt gccgcaggga
     9601 gtctgcccgg atcgatcgga acgctgcttg tgctgcaaaa ctacgcacgc ggtgcggggc
     9661 tcaatcggat cgtcaccagc tcgcttgggg tggcgctgat tttggtcgcg ctggcacagc
     9721 ttctgcgcag tacgctggtg cagtttggca aaaatcagga cgcgcggatt cccagtccac
     9781 tcaaaaaact gcggacgccg ctgacgattg cggtggggct cttgctgggt gtgctggtga
     9841 cgctgtcttc ggtgggagca ggcgcgctgg gaaccgttgc ggtgttggcg ctgtatccac
     9901 ggttgccgac gctgaaagtg gtgggaacgg acctcgcctt tgcgattcca ctgaccgcgg
     9961 tggctggact tggccactgg cggctcggtc acgttgactt ttcgcttttg gggagtctgc
    10021 ttctcggcag tctgcccgga atctggctgg gaagtctgct cagcgcgaag attcccgaac
    10081 gcatcttgcg accgctgctg gcgattttgc tgctgctgat cggtctcaag tttgtgatct
    10141 aaagaacgcg atcaggaagt cgtcaccgtc ggtttgggag ccgtttcaaa gcgcattccc
    10201 aagattgaca gaatcagcac cgcaccgcag ccgatcacgt tgtaccaaag aaaaccgatg
    10261 ctgctgaaag caaacaccag cagcaccagc gcttgtgctg cgacaagcga aacgaacacc
    10321 gacttggccc ccacgcgcgg caaaaagaag ccaaccaaaa acacgcccag cgtcggaccg
    10381 taaaagatcg atcccaagat gttgaccgct tggatcaggt tgtcgacaag cgatgcgaat
    10441 gtcgcaaaca gcatcgccag cagcccccac aaaatcgtaa atcccttcgc cgccaggaag
    10501 tagtgtcgat cactttcatg cggtcgcagt gacgtcttgt aaaagtccac caccgtggtc
    10561 gcgcccagcg cattgagcgc actggccgtt gctgacatcg cagcgcagaa gatcaccgcc
    10621 aaaaacagac catacagtcc agaaggaaag tgatctttca caaaggtgat gaagatgtag
    10681 tcggcgtcct tggtttccgc gcgcggattc gctttgcgaa cgattccctt ggctgcttgg
    10741 cgaagctgct tcgcttccga atccgcgctt tgcagcgact tcttggccga ctgcatcgct
    10801 tcgacattcc cactgcggcg cgcaagcagc agatcatcta tcagtttctg cttgcgcgaa
    10861 aacacctggg aataggaatc ctcgacgctg cgtagctcgg cagaatagac ggactgctcg
    10921 gcacgatgca gctctgttgc atggaaaaac agcggtggac gctggaactg ataaaagaca
    10981 agcaccagga cgccgaccag caaaatcaca aactgcatcg gaattttgac gattccgttg
    11041 agcagcagtc ccagccgact ttcgacgagc gatccacccg tcagatagcg ctgcacttgg
    11101 gtctgatcgg ttccgaaata cgccagcgcc acaaacagac cgccggtcat tccggaccaa
    11161 aacgtatagc gcgtatccag tcgcagcgaa aaatcgaccg cgtgtagttt tcccagactc
    11221 ccggcgatgt acagcgcgtc gggcagactg acctctttgg gaagcgcaga cagaatgaac
    11281 aggaatgcga gcagaacccc cgacagcatc acaatcattt gataggtctg ggtttgactg
    11341 acgacccgtg ttccccccga caccgtatag acaatcacaa ggaccccaat cagcagattg
    11401 gtcaggtgca gcggccagcc aagcaccgtc gacaaaatga tcgcgggggc ataaatcgaa
    11461 atgcctgtac caagcccccg ttgcagcaaa aacagaaaag cggtcagcat ccgggtccgc
    11521 cgatcaaagc gatgttccag atattcatag gccgtcgcca cgcgcagccg gctgaagatc
    11581 ggcacaatcg cggcagacag aatcaccatc gccaccggca gaccaaagta aaactgcaca
    11641 aaacccagtc cgtcttcata ggcttggccg ggcatcgaca gaaacgtgat cgcactcgcc
    11701 tgcgtcgcca tgatcgacag gccgatggtg taccacttca tgtcgcgacc cgcgcgcaga
    11761 tactgttccg ccgtgcgcac cgatcggccc ttccacagtc cgaccgatac gatgacggcc
    11821 agcgttccga tcaggatcag ccagtcgagc tgggtcatga aaacaccttg cccaaaacga
    11881 acaggagcag caccgacaga aacagcatgc cgagcgtcag cacataaagc cgaccccatg
    11941 acttgagaat cggcggcgga tcacaaggag tctggtgttc gctagggttc atgagagatc
    12001 aagttggcaa acagtcggaa cgcgccggga accccagccg gaagctgacg gaaaaacgca
    12061 agtccggtgt agacaaacga tccttttccg tggcggacaa acagcagact gccgcgtctt
    12121 tctggttccc ccggatcgcg cagtgacagc ggcgtttcgt acttttcgtc gatcttgtct
    12181 gcgaaataca gaccccgttc ctgaacccaa ccggaaaagt cagccgcagt gatcttgttt
    12241 ggacgctgca agatcggatg attgggaagc gcaaatgtaa ccggggcgtt ttcgtcggtg
    12301 acgcggtcgg tagaaatttc aaatggatgc ggcccgatcg gcacttgcag ttttcccaag
    12361 cgattggacg tgctgtactg cacgaccaag ttcccccccg cctgcacgta gtccatcagt
    12421 tttttgtaat aaaacggcag ccgcggattc acgttgaacg cgcgcactcc gacgacaatc
    12481 gcaccataag gggccagcgg tttttgcagg atctgttctt ccgaaagctg cgtcacatca
    12541 aagccaactt gacgcagcgc tgccgccaca tcatcaccgg cccccgcgat gtagccaatc
    12601 cggctgcggg tcttcttcag atcgaaccga cacagcgaca cgctcgcttc cggaaacacc
    12661 gtctgaatcg gaatgtgagg atgctcaatg cggacaattc cgcgatcgaa agactgtccg
    12721 tttgtttccg cgatgacgcg cagcgtaccc acaatcggtg tcgcttgcgg attggctttg
    12781 ggacggactt ggaacaggac ttcctcttcg gccccctttt tgtcgagctg aaacggaatc
    12841 gcgctgggtt cgacggaaaa tccgctgggg acttcgatcc gcacggttcc tttgacatct
    12901 gcttgcccgg ctttgagacg cacccgcagc ggcttctttt caccatcggc aaacatcatg
    12961 actttgccat cgacgttcac cattacctgc ggccacactt cgatcgatcg agtgcgctct
    13021 ccagcgacgg gatctgtcca tttgtgaagc accgcgcgat cgactgtcag ggtttccttt
    13081 tccccctgcg gcccgctcca ttcgatcaca aatgctgcct gggcaaccgg aggatcttcg
    13141 ggcatcccaa tccgcagcgg atcgcgcacc ggataaagtc cgggcagtgg ctgttgggtc
    13201 agccaatagg gattcccaat cggtgtctgg gccggaatcc ggatcgtctg ttctttttgc
    13261 cacagctccc cgcttgccag gttctgctcg acggacagca tctctccggt ggacagacgg
    13321 acttcgcgca gccgcagcgg cgcagtcgat cgattgatga cttgcaagct gaccgaaaga
    13381 tcactccctt ccgtgatcgc atgatcgctt gcactcgctt ctgcatacag tcccgcgcag
    13441 gccacgatga tgtcttcgat ctcacgcagt ttgggctgtt tccacggact gtcaggcagc
    13501 cgcaaaagtt ctctgcgtgc ctgcaaaagc tggggaatcg acgcttccgg tcgtcgcaga
    13561 tcgaacgact tgctgatctt cgccagcaac agcgcaagcg gtccgccacc cgccacccgg
    13621 ttccaagaca gatcgattcc ttcaaacaaa ctcgcgggca gcttgctttc cgatttggga
    13681 agaagcggca aaaagtattc tggacttgcg ccgcgctgct tggcagaacc aaatccctgg
    13741 ctcttgtgca tgctgcggct ttccgacgcc agttccccat aggaaacacc gagcacaggg
    13801 ttataggaac cgacatcaag cttgaggaac ttcgacaagt cttccccggc cagactgcgc
    13861 cacggtgatc gattccacag aagacgccgc gcctgccaaa cagaaacttg tgcaagctgc
    13921 tgcttggcaa agtttggatc tgccgccgcc gcaaatgcct cttctgccag catcgccgat
    13981 gcggtgtgat gcccgtgtgt atccagtccc tgcgtcggaa agcgcgtgat gatcacatcg
    14041 ggacggaact tgcgaatcac ccacaccgtg tcttccagca cggcattccg atcccaaatc
    14101 gacagcgtct ctgccggcgt tttggaatag ccaaagtcgc gcgcacgggt gaaaaattgc
    14161 tcggcagaat cgacgcgccg cgctgcaagc agttcctgcg tccgaatcaa accgagaagt
    14221 ggcccctgtt ccggtccgat caagttctgt ccaccatcgc cgcgggtcag ggacagatac
    14281 gcggtccgga cctgcagatc gctgctcaga tacgccagaa gccgggtgtt ttcgtcgtcg
    14341 ggatgcgctg caacatacag cacgtttccc accacagaca gccgatcaat cgcccgcaaa
    14401 agctccgcac cacttggttg tttgggagga ctgtgcgccg aagaaaccat gtctgtgagc
    14461 agtacaccga tagaaaggac gatgccaagc accgattttg gcgaaagcaa acggcacccg
    14521 ttttgaagtg atcggttttg aaacgacatg atggacaaga tgcctccgca gcagaaaaac
    14581 gcgcgccagt atacgcagac cgtttccgct tttgtgcacc aagcgtagtt tcgcaaacgg
    14641 tccactttcg gtagtctgcg cagcatcatg ctggcttcgt ttggtgcggc gtatctgaaa
    14701 ggcgatcctt tggcggcggg ttttctcgac ggtgattttc gcgatccgct gtcgcttcgc
    14761 gcgatcgtgg cgcagcggac aagtcaaagg atttcggtcg agctggcggc acttctcacc
    14821 acgcaacaaa acccacttgg ccaaagtgtg gcgcgcgaaa gacacatgca gcagctctgc
    14881 gcggctcagg gtgcgggcgt ggtcgtgacc ggacagcaag tcggactgtt tttgggaccg
    14941 ctctacaccg tctacaaagc agcgaccgcg gttgccgctg cacgcacact ctctgcgcag
    15001 acaggacagc cgattgtgcc gctgttttgg ctgcaaacgg aagatcacga ctacgaagaa
    15061 atccgatcct gtacgattcc aagtccgaac aaaggagagc cgccgctggt cctttcgctg
    15121 ccgccgtccg accccgcaga acagcgaatg tccgtcgcat atcgtcagct gggtccagaa
    15181 gtcgatgcgc tcctgtcgga gctcgagtcg tggcttcatc ccctgcccca cgccgccgac
    15241 tgtctgtctc ttttgcgccg gcattacgca atcggtcggt cgctcgccca ggctttttcg
    15301 ggactccttt gcgagttatt tgctgacgaa ggactgcttt gtttggaccc gcgacagcct
    15361 ggacaggaca ccctacccaa gcttgctgcg ccgctgtatc gaaacatcct cacgtcgcat
    15421 gaaacgctcg gcagcgcgct ccttgcgcga cagcgggcgc tttccgatcg gggctttgct
    15481 tgtcagattc cggtgcgtcc gcacagctcg ctcttgttct ttcatcccga cggggaaagg
    15541 agtccgcgcc atcgccttca gcagcatcaa cagaccgggc cagatggaca cgcttcgctt
    15601 ctgcagtggt cgattccgca ggacggacgc cgctattccc agtccgatct gctcgcccgc
    15661 atggacagcg atccgctttc gtttggtacg tctgcgcttg tccgcccgct tgtgcaagac
    15721 actattttac cgacggtggc ttacgtgggt ggccccgccg agctgagcta ctttgcccaa
    15781 gtgacaccgc tttaccatgc gctggggatt gtccagccag tcatcatgcc gcgcgcgcgc
    15841 tttcgcttga tcgatgaaag tacgcgcaca gcgcttgcca agctggcgct gcgtgcgtgc
    15901 gatgtggaag cgccgaaaga cgacatcatg ctgcggcttg cgcaaggaaa gccagccgat
    15961 gtgccgtcgc cccaagcggt ggaagagagg ctccttgccc agcttctgtc gccactttcc
    16021 gagatcgatt cacttgatcc ggcgcttcag gatgccgttc acaccgcgcg ccgcgtgatg
    16081 gaaaaaaccg cgaagaaaat cagcttgcgc tacgcccaaa ggctgcacga aaaagacacc
    16141 gtgaacagcg agcggatcga tcgtttgcaa gcggcgattt ttccttcgtc gaccccgcaa
    16201 gagcgcttgt tttcgctgcc gttttatctg gccaagtacg gactctttgg atggaaacag
    16261 agactgtttg aaagtctggc cgcaaggtcg gttttttccg ccgatcagtc ggtgcgtgac
    16321 atttttctgt aaccgtttgg aaagggaatc catgcctctg cgcatcgcgc tgatctgctt
    16381 tcccacggtg ggaggaagcg gcaaaattgc gaccgacttg ggacggacac ttgcgcgccg
    16441 cggtcacagc gtccatttgc taagctacga tagacccggc ttcatttttg cgccggaagc
    16501 aaacctgcac gtcgaaaaaa ttgcgattcc agactatccg ctcttgcatc tttcggccta
    16561 tgcgatgtcg ctcgcttcgc agcttgccga gctgtcacaa cacgttggct tcgatctgtg
    16621 gcacttgcac tacgcgatcc cccacatgat gagcgcgtac ctggcgatgc agcttctgcg
    16681 cgacagaaac gcgccgattc ccaaagtggt gacgacactg cacggaaccg atgtaacact
    16741 ggtggggagt gatcctgcgc ttgtgccgat gcaccgattt ttgctttcac agtgcgatgc
    16801 gctgacgact ccttccgcgt atctacgcga cgctgcacat gcgcagattg ggattccgca
    16861 gacttcgccg attcaggtga ttcccaactt tgtcgacacc gaattctttt gtccactgcg
    16921 gccaagtccg tcttcatcgg aaagatgcgc gcttctgtca cagcttctgg gaaaaacgat
    16981 cagtgaagag gaatctgcga cgacagcggt gctgcttcat agctcgaact ttcgtccggt
    17041 caagcggatc gatgatgtga tccagctctt tgtccgggta acgcagcttc tgccagaagg
    17101 tcggcgcggc gtcctgtttt tggtggggga tggtccagaa aaaccgcgaa tggaagcgct
    17161 tgcggaatcc atgggagtgg ccgccgcggt gcgttttttg ggaatccaac atgactttcg
    17221 cacgctgctt cagcacagcg atgtcttcct tctgccgagc acgatcgaag gctttgggct
    17281 gtcggcactg gaagcactca gctgcggagt tcccgtggtg gcatcgcgcg ctggaggcct
    17341 tgtggaagtg gtcgaggatg gcaagaccgg ttttttgtgt ccggtcggtg atgtcgccgc
    17401 gatggccgat gcggtgctgc gcatcctttc ggttccgaac ctgcggtcag cgctgtccca
    17461 aaacgcacgc agacaagtcg aggagcgctg gcagagtgat caggtgatcg atcaatatga
    17521 atcattttac atgcacattt taaggcacta tcctttataa agagcgatgc tcgccgcaaa
    17581 cagtgggatt gcgcgcccgc ttgttagcgg gtaggttttt agcccgcatg tcacttctga
    17641 aaattgttcg tttcccgtcg ccgtttctgc gtcagccgac ccaagaggtc aagacgatcg
    17701 acaaagagat tcgcacgctc gtttccgaca tgatggaaac gatgtgcgac gtaaatggtg
    17761 ccgggcttgc ggcgatccaa gtcggatcgc ttcaccgcat cttcatcatc gaagcgctcg
    17821 ccgcgggcgg tgagcggacg gaccccccga aggtgtttat caatccggtg attgaacaac
    17881 tgtcgcccga taccgatgtc cgcgatgaag gttgtctgtc gtttccgggg atctttattt
    17941 cggtgaagcg gtcgctcaaa gccaaggtct cggcgctcga tctcgacgga aaaccgtttt
    18001 ccgtggatgc cgaggagttc ttcgcgcgag cgatccagca cgaatacgat cacttggtaa
    18061 acaagctttt gtacgaccac gcaggtccca tcaaaaagca gatgataaag cgcaagctag
    18121 acaagatgac cgacgacgaa gcgatggatc tgctgcggca atacggcgag taattccgcc
    18181 cccaaaaaac agcacacttt gcgctgaaca aagcgcctgc gcagccgtct gtttgctacc
    18241 gtagacgctg cataggagtg tgctcaatgt ctcggactcg ctcagcattc ggattcgata
    18301 tcgctgccgt tacgttcgct tcctttctgc ttggcgcaac tgcgcaggca cagaccgcac
    18361 aaaaggtgga tgctgcggat ctcatcgccg cgcagaaagc aggcggaccc gccgccgccg
    18421 cgtacaacgg caagctgctc gaagtcttta acctcaagat aaatggcttt ttcccggtga
    18481 acgacgcgct gatgcgacgc aatccggccc tggacctgcg gacgtcgaaa tatttcgact
    18541 acatcgcgtg tgaatcgacc ctggccaatt cacctgagct tgcaaaactg gcgacgggac
    18601 agcagctggt cgtaaccggc gtggcccagg tgggaacgta ttcgctgcgg ctcagtggct
    18661 gcacgattgt caagctgctt gatccggtgc ttcccccgtc ggtaagcatg gccgatacag
    18721 gtggcaaagt gaccgccgcc gacgttgtca ccgactactt gcggaactcg gtggcggcga
    18781 cgctcaaata caagggcaaa gtcatcgcca tcagcggcaa gctcagcgac acaagcggcg
    18841 gcaagctgaa gctgcgcggc accgaattca actcggttac atgcagcgtg cccggcccgg
    18901 tcgccacctt tgctgccagc ctgccgtcgg gctcaagcgt ggtggtcaaa ggcaccgtca
    18961 gcgaaatgga cacgatggat ctccaggtca aagactgttc gctggtcgat ccagttcagc
    19021 cgccgccagc cgccgctcct gttgcggcac aggctgctcc tgtggctgca ccgacggctc
    19081 ctgtggctgc accgacggct cctgtggctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn gccgcagtga agttcaaagc cgctgacctc aagagctact atccgacggc
    19261 agttcacgcg ggcggaaact ggacgatgac gggcgttgcc gccggaatcc cagtgcaggt
    19321 ccggatggat gaaaaggacg gatcaccgac gcgcggccac ttcttcatca cgattgccgg
    19381 tgggaccgag cgcccgatga acaaaaccga gctgcggatg ttttacgacg gcatgcttgc
    19441 acgcgccaaa gcccccggcg cggcaccgtt tgaaaaagaa gccgcccgcg ccctcgctca
    19501 ggccctggga atcaagccgc cgaagctgcc gtaaccccga cgctcgcccg gactactcag
    19561 cagcgagaaa gccgtagcgg tagtcgcgcg gcggcacaaa gttttccttt atcgcgcgca
    19621 ggctggtcca gcgcagcagg ttcatcgccg atccggcctt gtcgtttgtg cccgatccac
    19681 gcgcgccacc aaatggctgc tgtccgacga cggctccggt cggtttgtcg tttatgtaaa
    19741 agttgcccgc cgcgtgacgc agccgcaaaa gagcctgatc aatcacggtg cgactgcgcg
    19801 caaacacggc tcccgtcagc gcataaggcg acgacgtatc gacgagccgc agcgcttcct
    19861 gccagtcgtc gtagacgtgc agcgttacaa ccgggccaaa aatttcttcg cacatcagct
    19921 tatgacgggg atcttcggct tccaccagcg tcgggcgaat gaaataaccc aggctgtcgt
    19981 cgctttcgcc gccgcacagc acccgcgcgc cgggaatgct gctcgccgcg cggatgtacg
    20041 acgtgatcga tgaatacgcc cccgcgtcga tcaccgcgcc catgaagttg cgaaagtcgg
    20101 tcgggtcgcc cactttgatc gactcgattt cggcgcacag cttgtcgcga attttgcccc
    20161 acaaaggacg cggcacgtaa atccgcgacg ctgccgagca cttttgcccc tggtattcaa
    20221 agccgccgcg gacgatcgct gcgaccagtg catcttcgtc ggcgctctcg tgcgccaaga
    20281 tgaagtcttt gccgcccgtt tcaccgacga gccgtggata ggtgtggtag cgctggatgt
    20341 tgttgccgac ggtgccccac atctgctgga acacgcccgt cgagccggta aagtgaatgc
    20401 ccgccaagct gggatgagac agcgccactg cgccaatttc cgccgcagga cctgcgacca
    20461 agttgatcac gcccggcggc agccccgctt ccagcaaaag ttccatgatg tagtacgccg
    20521 acagcatcga cgtcgccgcc ggtttccaca aaaccacgtt gccaagcagc gcgggcgccg
    20581 tcggcaaatt tccggcaatg ctggtgaagt tgaacggcgt caccgcaaag acaaagcctt
    20641 cgagtggccg cagatcgagc tgattccacg tcggcgtcgg gctcaacggc tgatccgctt
    20701 cgagcagccg ccggtaaaag tagacattga agcgaaaaaa gtcagccagc tcacacgctg
    20761 catcaatttc ggcttggtag caggtcttgc tttgccccag catcgtcgcc gcgttcagcc
    20821 gcatccgcca cggccccgac agcagatccg ccgctttcag aaacaccgcg gcgcgctgct
    20881 gccacggaag tgctgcccag tcggcctgtg cggccagcgc ttgcgcaatc gcttgctcga
    20941 cgtggcttga tccgccctgg tgaaagaccc cgaggctgtg ccggtgcgag tgcggcgtca
    21001 cgatgttgcg ggtcttgccg gtgcgcaccc gttcaccgcc gatgtaaagc ggcacgtcga
    21061 cgctggctcc gcccattttg gcaagctcgg cttttagcgc atttcgctcg gcgctgcccg
    21121 gtagatagcc aaacactggt tcgtttttcg gttccccgta tcgcgcgatg gtgtcctgca
    21181 taccgacttc gtagcacaag ctacgtgcag ctacgcggtc ttttgcccac ccggcaggct
    21241 cgatggacgc gccaaaagcc gcggcagcac cggcaaacag accagcgcaa aaccgagcgc
    21301 gacaaacgcc aagctccgcg caagccgagc gtcgggatca gcggtcacaa agcgccgctg
    21361 caagacaaaa aacagcgcgc tacacgcaaa atgggcaccg cgcttgagcg agttaaaacc
    21421 cgacaccaaa gacgccggta ctttggctcc ttgctcctcg gtgaagcgaa tcagcgtcgc
    21481 cgcgctatac ggttcgataa aaccccgcac aaagttaaac aatagacaca gcacaatgca
    21541 cagcgtcagc accaccaccg gctgaatcga caggttttcc gtcgcataga agcccagcgg
    21601 aagcagcagc aaaagcagcc acgatcccag ggtgagcgac agtgatcgtc gcggcagcac
    21661 aatcagcgct tcgccgcgat gacgggcaaa ccgctgcgaa ccgacgctgc acgcccagta
    21721 gccaagcacc gccaccagcg tgtaaagcgg ccacaacagc ggatgctgcg tcgccagcgc
    21781 cgtcgaaagc cggctgagcg gcgactgcac aaggtaaacc gcgaacagcg ccaccgccgc
    21841 ggccatcgac aaaagccaca gctcggcaaa aaagcgcgga tacccgagca ccaccgaaaa
    21901 accgcggaac agctcgcgca gccccggacg atgcagcggc gtatggtcgt cgcgcagctt
    21961 ggccagctcc gccagtgcga ccagctcggc tgccgaagtg agcaaaaaca acccagattg
    22021 caccgcagtg cgcagatgca gcgccggcac aagccatgcg cacaagtcgt aaaacagcgc
    22081 cccgagcagc accgccacca tcgtgcccaa atagcggatc gagctgccga cgccctcgaa
    22141 gctgggatgc aaaagacccg gaatttgcga acgccgcgcc acttgcaaaa acagcaccgt
    22201 gtcggctccg ctcagaagtg ccaagctgac gccgattgcg ccttccaaaa acagcactgc
    22261 ccacggtccc agcgacagcg cgacaaactg cgttggccac agcgcgcgca gaagcgccat
    22321 gccaaaaaac gccaccatga tgccgtttgt cgcccacagc ccgagcgaaa gcgacgcttt
    22381 ggccccatgc ctatcggcat aaaaacccgt cggcacatcg gccagcacca tcgtcaggct
    22441 cgccagcccc agcgacagca ccgacagtga cggatcaccg ccctggccgc ttgcatccgg
    22501 ccccgaaagc gcatcaagca gcggcacgag caccggcgca tgaaaagcga acaagtaagc
    22561 gaaccgaaag cgcaacagcg cccgaacttc ggggtgagcc aggcaggcat caatcgtcgc
    22621 acgcgacaat ccgcgcaaaa gaacgtggcg aagcagccaa tcgaacatcg ccgcttagtc
    22681 cttgccggtc tcgcccgtcg ctggatagcc gatcagccac gttacttccg acaaaagctt
    22741 gcgaccatgg ccaatctgcc aaacatacgc cggtccgtcg agcgaatcgg tcatcgcccg
    22801 cagttcgtct tcggtgtagc tgcgcagcgt cgatacaagc gcgtcccacg caatgacgag
    22861 cggcgcaatc ggcaccacat acgtcaacag cagtcgccaa aggcgcagcg gacgaaccag
    22921 cggcgtcacc agaagcacca caaacggaat gagcagcgcc agcacgaccc ccagcagtcg
    22981 tcgctgcgtc acttcaaaaa tcgcaatccc gcgttgacaa cgcaccgcgt cttgcaaaat
    23041 cgcccgcgcg gtttccggcg gaaaatgatg caacagttga aacatcgtcc gcagtcccgc
    23101 cgcgtgggcc ggcaccgccg tggcatcgac ggaatctggc aaaaacgaca gccgctcggg
    23161 atcgcgacgg accgccgcag aaagcgcggc atggtttgga aacttgtcgc tcatcgtcgc
    23221 ccgcaccata agcccgtgat ctttggcaag accgtcgaga agctgcggca gcggccccgc
    23281 cccgcccgat ccaagatcga gcagttccac actcccggtc gcccgcagcg ccttggcgag
    23341 catcggtaca gcgacggaat acgatcccaa aaggcggaac gcggcttcca aaaactgcgg
    23401 cgtcaggctg cgaaaaaacg gcggcagcca cggcaaatcg caaaactcga acaggtgata
    23461 gcgcttcatg ccgcctcgga aacaccaagc gccgcatcaa gctgcgcaag cagaccgtcg
    23521 gcaattcgcg tcgcccgatc cggtgacacc agcggatcga caaacaccaa gttgaggttt
    23581 agcctgtcgc ccaccgtcgt caccgccaaa agaagctgtc ccaccaccga cagcccgacg
    23641 gtgatttggt agtcctcaat ctgcaagtcc ccatattcga gcgggatcgg cagcttttgc
    23701 aggtttgtca cccccgtcag cgccggcgaa gccgcatcga agtttttgac aaagcgcgcc
    23761 gccacgtcgc caaaccgcgg aataaacagc ccaatcagcg gcatcgtcag gtactgctcg
    23821 ccctgcgcaa gcgtccgggt cagctgttgc cgaagttcgc gcgccaggtt ccacagcgac
    23881 ggcggcggca ccacctgatg gaacgtcgtc acctgcgaga tgtagagccc catttcttcc
    23941 ccgacgctgg gcgtaagatc gccgcgcagg ttcaccgccg aaaaacagcc ggcctttgtc
    24001 gctcggtttt gcgcaatccg ctcggcgaat acttcctggc tcgtcgacaa aagcagcgcc
    24061 gctgccagcg caccatgcac cgtcgtctgt tcttttttca cccgcgcttt gagcgccgta
    24121 agcgtttccc cgacgagcgc ccgatgcacg acgcgattgc gacgctgcgc cgctggcatt
    24181 ggctgggaaa gcggcagcgt cttggcccga cgcagcggct tgccgacgac atgtttgaaa
    24241 aaaaagtgat gcatcgccgg cagcagccgc agaccgcgcg ccgacggtgg caacagcgcg
    24301 ggcatgggcg gtcgcagcgg caggctttca ggtggtggca agatgtcgcc gcgacgaagc
    24361 gtatccaaat cggtcagcag atcgcgaatc gcaaaaatga tcgagagcgc atcggcggtg
    24421 acgtgatggt gcgaaaggat cagatcgctc cgatcgtcgc caagcaagag ctgaagccgg
    24481 gttaagtgat cgccctcacg gtcaaacgga cgatttagct cgtcttcggc gagcgcgatc
    24541 cagtcgctgt cgctgcgcct tgtctgcacc gtcagcggca gcggcccgtg atcttcgtcg
    24601 gtcagaaaca gctttttgtc gtcgccacgt agccgcaccc gcaaaagtgg atgccgcgcc
    24661 tgaagccgca acagcgcctg ccgcaacagc gcgacgtcga ccgccccccg cagcgtcacg
    24721 acttggacgc cgttaaagcg ggtgccgcga tcgagcagat aaaaggcgcg ctccatgtcg
    24781 gccagggctc gttccatggg cgcagcctag actttgcaac caatgcgatt caaggcaaga
    24841 gcaggtaata aagcagccca ccgacgatcc caccaccgat aagagcaagc ccaccgagca
    24901 acagcagcgc cgacttccac gccttctgat ccgcagcacg gcggtcgccg tcgagcagtg
    24961 ccagccgcgt ttcgactttc gcaattccgc tgcgcagcgc atccagcacg gcatacagcg
    25021 aatcgaatcg cgcccgtttg tctttgccaa aagccggcag cggacgaacg ctcgcacaca
    25081 gcgtccccag caccagaaga tgcggcgact tttgcaccga ttcggcgtat tccaggctca
    25141 gcgcatagac aagctccgcc cgacgagact tcagatggtg atttttccgt cgcaccagcc
    25201 gccacagggc gcgcacttcg cccagccgcg ccgccacttt ttgccgatca ctttcaatcc
    25261 cggtcagctc atttccgacg gaaaaacgct catcgccact ggcttcgctg gcatgcggat
    25321 caccggccag caagagcagc cgctgataca gctcatcacg gcgatgccgc aagacgcgca
    25381 gctccgacga cagcaccgct tctttgcgcg ccagtgagcg ctgctctttc tgcgcctttg
    25441 cccacagccg atccgccacc acttgcaccg ccagcagcgc cgcgtctttg ctggccggca
    25501 gctcggtact gactgccccg tcgctgttat tcggatcgcg caaaagttcg atgttttccg
    25561 cataggccag ctcacccagc tggaccagcg cgcgatcgta ggcggacagc cgctcgccca
    25621 ggtcgcgcag caagcaccga agctgttttc gccgacgcat aagcgatcgc agcccagaca
    25681 ccgcatagga caggcggccc cagagcgtgt ccgacggtgg cagcagcggc agcggtggca
    25741 gcgtatcttc actcggcggc ggcagactga ttttgggcag cgagcgggta cttaccgaca
    25801 gcacactctc tgcgtcgcga tcgtcggacg gcaccatcgg catttccatc gtcgcggcag
    25861 aagccagcgc ggcgcgggcg atttcagcgg tgctttcgcc cttatcaggg tcggacggcg
    25921 acgggatttc agccgaataa gacggcggcg gtgcggtgat cggcaccgat cgagccggta
    25981 cgcgctgcga agtcggcagc gtcggaaaac gcatcgtctg agacgacgga accggatcca
    26041 gcttggccgt ttcatgcggc gacaccggca gcatctgtgc ggtcgcattc ggcagcaccg
    26101 cctggaaacg cctggtcagc gagcgtggta cttgcggcag ctcgacggtc gccgccgggt
    26161 cgatcggcac aagttcctgc gtcatcgccg acggtggaat cggtcccaca tccagccgct
    26221 gccgtggcgt ttcatcccca cgcaatcgcg atggcagaaa cagcggcatc tgctgggttc
    26281 tgtcgacggg aagttcggcc atgacgcgcg cgatggcatc cccgctttgc gcagccaacg
    26341 gatcagaaat cccgtcgata ggccgggtcg cgaggaagcc ctgcatcgtc ggaacgtcaa
    26401 tgacatgttc cgggatcgca gactgttcgg acgacagctc cggcgacgat ttttcctgtg
    26461 agagtcgcgc gggcaacatc gcccggtcgg cttctaacag agcctcgtcc ggcggctcat
    26521 gttcgggcgc gagtagactt ctggccggcg gcggcagcag cgtgtcttct tcgaaatcag
    26581 cggcaggcat ttccgtcgga gcagcagcgg gcttttgcgg catgaagcac gtcctcgcca
    26641 gggcagcgag ccaaagagac cgcatcctat tacgagttgc ttgcttctcg gaagaaacca
    26701 agcgacccct tgcgacaggt ggggcaaggt cagagaacag cgacggaacc gcgattttcg
    26761 acggagaaga ggggcatatt acttcacagt atttttgtta gctaaatcac tctacaattg
    26821 aacaacagga aaaattttcg taaggtaatt ccagtgacta ctgcacgaag accacgatca
    26881 gaggggcggc tggcgaagta tcttcgccgg tgtgagcggg acttcatcct gaaggcgctg
    26941 agaatgcatc gcggccacaa cagccagacg gcggcgtggc tcggagtttc gcggcgtgcg
    27001 ctgtacgaca aaatgcgcga ctacggactc gatggtgagg cgtcggcgat gcgaaccgag
    27061 gctggcatca tggggccgcg caaagtcgac ctcgacggtg gcgacgagga ttagcgttcg
    27121 cccggcgcag gcagcaagct gactatctcc aaatttccga cggtgcgaag cagttatctc
    27181 gcaacacacc gacgaaaaga gagacgcagc ttgcctctgt tttggtaggc gatgaaagcc
    27241 gctgcatgtg atatgcgtgc ggcatgtcag aaacgcctcc gaccgactac gagctgtact
    27301 tgcgcgttcc cgagctcctt cgcttgcaaa agcaaccgag tgagctctcg tgccccgacg
    27361 agcttctgtt ccaggtcgtc catcaggctg ccgagctgtg gatgaagctt gcggactttg
    27421 agatgcgact gctggggccg ctcatctcgt cggaacgact tgccgaagcc agcaagacgc
    27481 tgcgacgggt tcatgcggtg ctggtgcagc tcgcgcagag cctggagacg ctgtacacac
    27541 tgacgccgtc cgattacatg cggattcgcg aagtgctggg acgcggatcg gggcaagaat
    27601 cccccggatt taagcgactg ctcgcggtgc cggcagaagt ctatccgcac tttgagcagc
    27661 ttttggccaa gcgcaagctg acgctcatcg aactgtatcg cgagccgtcg cgccacgccg
    27721 acgtgtttca ggtcgccgaa gcgctgattg acttcgatct ccgtctgcaa gagtggcgca
    27781 atcggcacat cctgatggtc tatcgcacca tcggcgtggg cacgccgtcg ctcaaaggca
    27841 agcactccga aatgctggag cggggccttc gccagcagtt tttcccgaag ctgtgggaag
    27901 tccgcgacga gctatacggc gaatggtcgg ccaaaaatcc gtcgggcgcc gactattcac
    27961 tgaccgggac cgtcaagcca gagcccgccc ccgtcgaaac cccactgccg gcgcgagcgc
    28021 ccaaaccgcc ccgtcgcaaa accaagtaaa gccgccatgc acgagccaac ctccggttca
    28081 gataacgagc agacgctgct gtcgctgcgc agtgaatttc cagcgcttgc gcagagcgtg
    28141 tacatgatca gccattcgct gggtgccatg ccacgggcga cgaccgaagc gcttagcgaa
    28201 tttacgtcgc tgtgggtcca aaaatcgatt gtggcgtggg aagactggct caggcaggcg
    28261 cagcttgcgg cgagccgcat cgggcacatc atcggcgcag cgccgggcac cgtccagatg
    28321 gcgacgaacg taagccaagt gcaggcgacg atcgcaagct gtctcgattt tgccaagccg
    28381 cgcaaccgcg tcgtctacac cgacatgaac ttcccgtcgg tcagctacgt atgg
//
LOCUS       DAORGN010000047        28344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_636_length_28344_cov_5.644491, whole genome shotgun sequence.
ACCESSION   DAORGN010000047 DAORGN010000000
VERSION     DAORGN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28344
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_28344_cov_5.644491"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="PKL17_07525"
     CDS             complement(<1..1951)
                     /locus_tag="PKL17_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNK44615.1"
                     /translation="MVSSIVFLVLLLVASAVFLRSVLRLYRIMARGKENDLKLTDRIA
                     TRIASVLIYFFGQKKVAEPMSYATPAGATSKHHLLIFYGFMIITIGTVEFMLQGLLWD
                     IVPGFSFRMILGPVYPALSWSIDIFNGIVLCMIFYAYFRRVVVKPRLIPLSGDAAFIL
                     AQIGMLMVSHFGFHGFHYASAQNPESFGPLSDAIGKATMGMGGAHVVSEVSRWIHAGI
                     LLFFLNYLPYSKHIHILGSLPNIYFRKLDQKGVMPKLNLDDENDWGVGKIEQFSQKSL
                     LDQYACTECARCSNYCPAYNTDKPLSPMHLIHDLKDEMKERGALAMQLDALKAQAGVT
                     AKDKSEAEAQLEKAPEAFKKQIADLQAKCDELPPLVGGRIKDETLWSCTTCGACQEVC
                     PVFIDHPLKIQQMKTHLVLTEGRMPTELQRTFQNLERQGNPWGVNADQRMEWAEGLDV
                     PVPTVEEKPDFEYLLFVGCAAAFDDRIKKSMRALVEVLHAAGTSFAVLGKSEGCSGDP
                     ARRAGNEFVFQEQAQANVDAFNEAKVKRIVTSCPHCFHTLKNEYPQFGGNYEVLHHSE
                     LLAHLLSSGKLQPDTKMNQKVTFHDSCYLGRWNGVYDAPRTVIDAVATGVIELGRKKE
                     RGFCCGAGGGRMWQEEKGPRVNRNRT"
     gene            2056..3846
                     /locus_tag="PKL17_07530"
     CDS             2056..3846
                     /locus_tag="PKL17_07530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK44616.1"
                     /translation="MPRATLRVRDLPDPLTFVIHPGTALLIGRMPDPERLRREHPDLA
                     PVLRDRRVLTTRIDSLRVSANHLLLVCTDEGEVSLYETHSRNGTSLRLAAGQPVQLQG
                     DVALDLAPRGGTTTGIGEPRPADWSDEGDFGSSVLEAINEWLRPQTVDLRAELRASGK
                     QDSDGWTFHLADDHELRIGSLSSGTYDPQVGVVLDRIRVYVHEQNLRFEQFKRRVDGM
                     IVSSPIMRDVLRRVSEAAEQGRRTVLLGPTGAGKEWLARCYHRNSPRATGPFAALNCA
                     LLDRDLLFAQLFGAKRGSFTGAVNDIPGMVEAAHEGTLFLDELGEMSLDVQKALLRFL
                     DSRGEYQRLGDPKPRRADVQLVCASNAKLDENDFRMKHFRDDLWYRLSSAVIRVPPLR
                     ERPEDVLAFLQLKTLRGSQLRVVDALSAVALKSVLSDPLPGNFRDLENFVDRLPLVQK
                     PGEIDVATVESALREGRGTPSSSSAVPQEGFQVKDSGRFKRFRQPQDRPPDNRNLDWD
                     GMMSAAVAAFLADHGDSTTSWGELQSLVEKYVKPVFVAYTTEMAYVDELSKNVNYSAL
                     ARKLNIADGSTVKMHLSRYVERFRQKKPGP"
     gene            complement(3788..5275)
                     /locus_tag="PKL17_07535"
     CDS             complement(3788..5275)
                     /locus_tag="PKL17_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44617.1"
                     /translation="MSRRLVPRWTVVVPWLALVALALVFRLSLVAEFRASAGDGIHYH
                     QLAQQLRHAHRFAYGPPPQPLTWTRSPGYPAFLAMLSPSTPVDLPQHLVIATRANAVL
                     DVGTAGLVAVMAWLLGMGRLAQVCGFASTIFMPPLFMLASHGLCESLTTWLLTLACCS
                     ALATRRAARPFFFALVAGLSLGLALWVRLDSVLALPALLVVLWMAGRTIKHRLRWVLA
                     CFALSMMVYSPWPLRSLLTFGELHLGPSPWIDTQGVPMRLGMMRWAQTWATGRIDGEN
                     YCLFVAANHGQLPPTRPNIILPTMYDDDAERQALFDLFNRYCREGESPDVDRGFYALA
                     AERLRKHPFRTLVILPLYRIRTLWSPMPSYELSLRSRLLRLPTQRPFWDNVCSALLLL
                     SGFGACIALLDPGKRRWAAALLLIPLVRSLVFAFLLPIPLQRYFVEAIPVLLLFSGYA
                     LAWPLVRLHDLLPKPLYRLWGQRPGGAMGPASSGEIAPRSDSDAF"
     gene            complement(5315..6868)
                     /locus_tag="PKL17_07540"
     CDS             complement(5315..6868)
                     /locus_tag="PKL17_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44618.1"
                     /translation="MWRFLAGIAVLFVSQLAWADLPALRSLAGDRKSTAMIDQGRQAL
                     ENNDPARAILVLEDAYRYWPKPGILYLLGRAALLEGRTRSAIDLYRRYIEQVGDDAEA
                     EVRAELTQVAQQPLEVGADLTVAGVPGGLVTVDDRLVGSLPLATPVQLSAGKHQVVLT
                     KEGRKVEATVTLLPRRAAEVRFTLKPPLAVTTLTPGVLLLIEPQSLEPGFLTNLRKTV
                     SEAVGRQRAVLVSPDTQASVMAQNRELEGCLDQPSCQEKLAMQSNATYALRLEFQLGA
                     AALSVPELQSGSSQRPVVAYRYSASVLDVEVGAIAATATDSCVASACNKLMSRIADMV
                     GDLLKTAANKPRGTLLVSSEPAGATVKLGPRNIGSTPLRRDAFIGPYELVLEKSGYYP
                     LVAQVLVEDNRELKLDRPLIPLPVVAPSRSRAILKWTTLGLGIVSAAAGATLLGLNGR
                     PMACSSDPTMTCPFDGRAGGAALLTIGTLSLGGSLALFLLDAQKPASSQTAMQPTSAT
                     PALSLSGSF"
     gene            complement(6913..11877)
                     /locus_tag="PKL17_07545"
     CDS             complement(6913..11877)
                     /locus_tag="PKL17_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44619.1"
                     /translation="MDSKSYSVGAKLADGPLGALHEGTYAQTQAKVLMRVLPAQLNKD
                     GRALSRLKGLRKSVSKLISGQSQVEPGARPDPVLPALLEYGNLPGGVVFLASEWVSGE
                     PLQEQRRRSMGLPLPNKALRIGRQLATCLTLAHSASLCHLRLSPSKVVVSTSAGEGEQ
                     IKLMDLGIAQSLLDLLPGGVISDVKGSDERVYLAPEQRNGELGTTASDVFALGVLLYE
                     LAAGRLPSQASLDESGASQVTSLPQILPSWQRPLAVLLDRMLATDPKARPTMGEVAAQ
                     LQQLSSQQPASSSSLPPPVHAPSPVQPASSSSLPPSSRSLPRNVSPPATPDVLSDSLT
                     IPGQVATAAKADVKPQTLPAASASSTAAAKDAKPDSLSDGLTLPGDRPKGDSIGFAQT
                     MVPPTVQAGRSAPAVPPVASSDAMSPAVEISAEYTQSGGVSTSAKTGDGGTAATSAEP
                     GVAGQVALRSGQLVGNYRVTAKIGQGGMGSVYSAVHKQIGRRAAIKILHGPLAGTADY
                     AARFLNEARAVNMLRHPGLVEVFDFGQLPDGTLYIIMEFLEGESLRGLLRRDGALPEE
                     LAKDLALQIGQALLAAHQKGIIHRDLKPENVMLVPDPLKPDERRVKVLDFGIAKVATS
                     IAADPESPDFETQVGTTMGTPKYMAPEQYGGAAKVDGKADVFALGVMLYEMLAGMPPF
                     PKTSLMAFAKPPRPLKEVAPTVSPKLAEYVHAMLAPKSEQRPAMADVVRELAPPPMTT
                     AQVAVVPAPRRSPIVWVLTGLFLACAIAAGVFVYEEHFRPRPATDLVVDANDLAIDVN
                     AARARALSVLYVGLRAPDPQLRAQAVRALGHSRDSAQWTSIVSLLKDPEPLVQVEAVS
                     ALGTLGALDAQPALLQLLDSNPNIGLKVAVAGALAKLQSQRGRAVLRELLQSSDERIR
                     LRAALLLVESGGDAATARPVLRDAVDKPGLPEDVQLRVLSRLAQTGDAQAVARLREAL
                     AGDEFSLRRVSAAAGLARVGEPSGKELLRQATQKEGPTQLLAALMLATVGDSLGFVRM
                     VQVATSASYPEPSRLVAIDGLSACGRRQGAQQLLKVLDEAKASQLLRQAAAGSIVQIA
                     GGDPEQIAKQSLGWAQAALGHDDWLVRESATLVLGDIDNADSVPLLTKALSDPQQSVR
                     RGAAAALGRKRVRSALLALRQALGDAEPDVRRAGMMSVSRVAAHLLSRGANAHDDELI
                     LRLRQLVETGNTEDQLTAAGTLLQLGEHAHRERLREGLQAPDPLTRKLVVEMLPAGDE
                     LLRHALADQALSVRFAAARKLIGHKVAEALPVLHEVLASGGVDSLVAYGLLKKAGEKV
                     SPPASFSELLGRGDMPTSLAVLDIIADLPLPEALPLLQKARLDSSPEVRRRVGEVSYD
                     FYKASPSEPLLMLMIGLLADPEMAVRTRVSSLLSQSREAQEEPPGDLGVPPDLTTVDA
                     GPTVQTGLLRIEGDDKVRFQVDDQAAQLLGPGMAAVTVPAGKHKVRYAGGAIDVEIVA
                     GSTASVRIPILLGDQFVIEAAEALSRHETTAAQKLLDRARALGSRGKLSRAMFAELTY
                     QQARIHEENGSHLEAMTEYEALGRLPERRPEHTTAALGASSRLQPYLGRLIISKEVDG
                     QCTTIMQWVRPGEHLVDGGRPVRVRAGGQARVELCASGRIDSSP"
     gene            12082..15123
                     /locus_tag="PKL17_07550"
     CDS             12082..15123
                     /locus_tag="PKL17_07550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44620.1"
                     /translation="MSRSSRSVPPGPGDGDGPPSKHELGLDVTGKRSDLPRDTDPDPN
                     AKVTLNPDLASATLREPGLAVETPPVSSRPSSDLAATSEQLADESALLRKQMTRSKPT
                     IEELPRGATIGRYMVLSCLGSGGMGVVYAAYDPELDRKVAIKLLKSSSSGSSSRVRFL
                     REAQAMARLSHPNVINVFDVGTIENQVFIAMEFVDGGTLNHWLTDKPRTQREIIDIYT
                     LAGRGLQAAHAAGLVHRDFKPDNVLISREGQVRVMDFGLARSIGQSEDDTTLPRELTS
                     SSSNQALNIRLTHTGAMMGTPRYMAPEQYEGQATDQRTDQFSFCVALYEALYGVPPFV
                     GDDINTLGFNVVRGRVASPPPDTKVPTWIRHVLLRGLQVKPEDRFPSMEELILALNPQ
                     TKRLPRPWLVTFVLLSLVLVGNLIYQVREQRRAGMCGGSEEKLRPIWSSQRKSELEQA
                     LLSSKQSYAQDVWQRVEQTLDRYASDWSAMRTEACLLTLRGQQTQQILDLRMRCLDDR
                     LAELRALTAILQKADPTVLQEAASATHELSPISGCANIEALSAPIPLPDNPVTRKRVD
                     ALRQEMAEVRAIERFGKYSEALVRAEKLAHDSVQSRYKPLAAEAKYLLGEIQMRGGLG
                     AQAEQSLVSAVMAAIDSRHLRVMAESALLLTETTGFRIKQFRKAEVWSQIAEAMLEEI
                     SDPEPLRASLYLANCMVASQENHYERAVDQCQKALSLREKLFGHEGPEVAEALNVLAG
                     VYRRSERLAQAIDLYNQALRIETAQLGSLHPNLVSTLRGLGIVARDQLRYDEALSYFQ
                     RALSIQEKSLGADNIRVSDYHIQLVRTLLAMNRLDEAEQHAEKAVSIRRKADARVDVE
                     RLTEAIYFLGEVKSARGQLHESLKLHEEAMRLREQQRGDRREEMLAFSLTSAGEVLFQ
                     LGQAAAARPYLERALNFRETQPADTRRAERSALGRTQFAMAQVLWALGDLEQQKQAIE
                     LAQAAAREYEAYGSRPGNILPKITTWLDMHPLPTTRKNHAR"
     gene            15113..16195
                     /locus_tag="PKL17_07555"
     CDS             15113..16195
                     /locus_tag="PKL17_07555"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK44621.1"
                     /translation="MRADLPSLLPLICPCCRHLSARGRELYSLSVGEVLRSTGGEDDG
                     TPEDIEEGILVCDNPSCQRRYPIIAGIPVVLPDLTGFASKQLLALSCFHEPETLATFA
                     AQGPDDAALPRMLEYLSIYLDAHFGDYSIPAPDGPEPGCGGTQLWHALSGCNAAPVER
                     AIELGCGVGRGLHILAEGAELTVGVELNFAALLYARWILRGETVRYARRTSGRHYQPA
                     SIHAPARSASAVQLVCGDALDPPLAPDSFQRVASLNVMDAVQSAHQHLSVVDGLCVPG
                     GEVVLSSPFNWQSGIVAEDGRLDAADPAQLLHDMLQSGRGLLSGYAISESRELRWRLR
                     RDGRSAVSYFTHYLRAHKRGQLPRPT"
     gene            16208..16858
                     /locus_tag="PKL17_07560"
     CDS             16208..16858
                     /locus_tag="PKL17_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2621 family protein"
                     /protein_id="HNK44622.1"
                     /translation="MVVFGLSHIDVPVVNLDRAEQWWCERLGFRVKTRSDGFLDIDVN
                     TCIVRLSKVRRVEHPVTLRIQVDHVESALADIKQVGGRVQYEPLRTAEQELVSAVLDP
                     DGHTLLLWRPLSEDEYEKIPELPKEMTWRPDAEELLQTLLRSVPSLFRAIARWRVAKN
                     AEYLAESTKVVHREQVIRAFILSSAKITRYRNRQPLIDAGIDVSQYEADFAAEERL"
     gene            complement(16890..19199)
                     /locus_tag="PKL17_07565"
     CDS             complement(16890..19199)
                     /locus_tag="PKL17_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44623.1"
                     /translation="MQFVSNANHPNRHRLWLLSLLLLLSQLASCGGEQFVLVTVLGTD
                     AETSGFVASARLDGVLVVDSQRFPSNPPYFHLTLPSSSQGQLEVLIEALDGDACTRAQ
                     GRGAVRIDKNGQATVDVFLTKQPFATCPVRVLLPDAAQSRVVSMGGELDCTGSCVRPI
                     RKGSSLSLHVDPATGEHLLGFRLPCVGTEPCQVTVSKPTDVEVAVVKAQVCSPDAVCW
                     DRPSPQGQTLRGLWGPSPRDIWAVGDAGSILHFDGISWVSTAVVSSETLNGMWGSATD
                     RAWAVGEHGAILRYDGKQWASVASPTIESLRSIWGSSGSDIWAVGENGALIHYDGAIW
                     SQQSIGTTMAFSAVFGTGPRDVWAMGYGSQSFHYDGLKWNAVPMGSTLLVVGIWGASP
                     SDLWAVGYDYTSRTGVILHSSGQTWIRYFATSGPRLYSVFGSPQGDVFAVGELGMLLR
                     LAKGRWETLPPLTTNRLLSGALFSTQLGLAVGEGGTTLRFDGLAWKLASDGDITNLFA
                     VSASEASDLWLVGDAGRVLHAHGGLFDRVPSTTTDPLRGVYAAKSDDVWIVGDFGTVL
                     HYGGQRIDLVTSGVSTDFRGVYGFGSKDIWMVGRAGLLLHYDGQGFARTDTGLTQALH
                     AVGGSSGSDVWAVGEQGVILHLVDGEWQRSWSRTGDTLRAVWAASPSEVWIVGDNGVI
                     IRYNGSSYEYDFVGDERNPITLLGIAGQPGGEVYIVGQSGKMFRFDRTAWRAVESGTS
                     GALSAVAKDPSGKYWFAGQGGAILHRDPK"
     gene            19416..20195
                     /gene="rpiA"
                     /locus_tag="PKL17_07570"
     CDS             19416..20195
                     /gene="rpiA"
                     /locus_tag="PKL17_07570"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNK44624.1"
                     /translation="MSQVESSAAVPASNPSPTAVARRAAAEAAVALLKPGMTVGLGTG
                     DTAAYAIRALAARKLTGLRCVATSTRSADLARELGLPLFELDDIAPATAATEKQPIDI
                     TIDGADEIDGVLQLSKGGGGALLFEKLVAQASQSMVVIADEQKVVRTIGDLRPLAIEV
                     VRFGALHTLARIAQLPQVQHAQLRRVVGSEALFHTDSGNLIVDAQLQLPPSTDAARTL
                     HHTLKQSLGVVETGLFCDEAALALVGQPDGSVRRLSRQSSK"
     gene            20232..23996
                     /locus_tag="PKL17_07575"
     CDS             20232..23996
                     /locus_tag="PKL17_07575"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNK44625.1"
                     /translation="MEWLLIPAFGVLIWLLQRSVQQEQALSTLAKRLKSTEELATYQA
                     ERAAKEVERLRTQVLELLKAHGQLQRRLGDIQEVVNAAPEPSHAQPIAPAVVVEQSPS
                     VQRTPPPLLPSTTEPLVVEPPVAASMAPEAVETTRTLADAAFQAAASAGLTDSAAGAD
                     TTDPATAPSRPSQPAPADTIDSEVAQAVPPAPAAIPTPPQPSNPAPPPAIVPPSAPTF
                     DWEQLLGVRGAAWLGGIALAIAGTLFAKYSIENGLIGPTLRVILLTLLGLAALVGSEF
                     FMRPRFAVTANAACGAGIAILYTAFYSAHGLYDLLPMAPTFGLMLLTTVVAALLSLRY
                     DSLYIAVLGLVGGFATPLALSTGQDRPIGLFAYVLLLNFGFLWVAIQRRWHKLLSLSL
                     AATLLIQTGWAIVHLSPEKLPILVATSTVFALLYISIPLFVKQFEPEKHQALLWNATS
                     AAIWPFLFAIVLATRPEYAQRWALLFALVVCLDIGLLILAVERWALLLWPALFGSILT
                     QVSWASTDAAVSRLPVLAGVSLLLMVLFGLAPMVHRFRKPSQEIPESLHLTAAGASLW
                     PLVAGLLLLGSDFGQRHGLLYGWLLVAYLWAAVTGTREGRVLRIIGVSTLLCLLGVQW
                     TQRFVSANVLWPATLLLMVPVLIGNLLPRLAPHLCQKDVDPQKSGTRATLHELAATIL
                     IIGVVGLLGVQIAKGLAEPPWLLLLVVTIVFGLLGERSRVSALPLVQPVMTLLVAALL
                     CGWQFGVFDSDADLVKVKPAERVLRDLAVPLLFAAAMQLRAAVRSLRGSAVAPALEDL
                     GPGSREQMIWDSEVSATVATLVGMISAMLALIDSGLSQRPGLALLTLLAYNLLLITTM
                     LRQTVTLLLLVGQLLSAIALISWQHDHFQAVDFPTVMPLYVVFYLWFLLLPLGLLLFV
                     PRLRARRSLFFAAGLAGPMFFYPLYRAWLSGLGDLVIGALPVVMATLSLAGLAIVQAL
                     PRPGVTRKLSADEMAERHLAHRALFAAVGVGFIALAIPLQLQKQWIGVAWAIEAAAVW
                     WMYRRLPHPGLKYFGLLLYCGVIFRLIPSAAFLQYHERGWPVVNWLLYSYGVPCICFL
                     VGAAGLAPVEDKYRRRFEDTLPFGRLPLAGIAYYTGLVLIFVLINLEIADAFSVGRYT
                     ELWQERSYARDLTRSLSWVLYALTLLVTGMRQSGRGQRFFSLGLMLLTVGKVFLYDLA
                     KVGGIYRALSFLGLAVSLFVVSLLYQRLAFRNAASGKDQNSKAPSPEKPT"
     gene            23993..26269
                     /locus_tag="PKL17_07580"
     CDS             23993..26269
                     /locus_tag="PKL17_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44626.1"
                     /translation="MSAFSHHPTLRRWVQLASLPLVLSLLGVLIQPSHAQGIATDAAQ
                     SSFPFRRSVEVRGSSMDGFAVFALPPELSNGDAEGGYRLIRSDGREVPYVVTSDAGES
                     KDRRHSGQLVDTRHEGDQTLWIVDLGSIRRFESLDLNIPQIQFRRRVTVEAAEQQNGP
                     YHVIARDVGIFDLPWQNAPGGRLHYTTVRFAESQRARFLRFQISSRWRGFLFRPLSLQ
                     GVAAVAHLSRPGSEWTLPAPLVPMATPPLPKHVKPGDHPHLSLYRIDVPPGLSFRHIQ
                     LSARDPGFVRSVRLLEIEDNASTVHSSTGKIQVLSEGMIFRISDGPVPTSGGKKADKG
                     EKTDPIDDEPVTGENLTIPVEDRPGKGILVLEITDGDSPALTDLTATVSGIGSRLIFP
                     VGPSASRESDSAQKYALYFGSETATKRDYDLQKLVSSLSRLGSFLPAQLGGKEPNPRF
                     QKAQPLPAVPTAGSPIVTSSYRLMRTVAMRDGVELYSIQLSPVDIAYLRPDFGDLRVV
                     DPDGQQVPYVLIPNAIETRTELEFAKEPAGDGKMSRYRLSLRHGNQLLQVPIDALELS
                     VGSSFYRRTVRIREVRSEATPYASQLLSATILRLSEDDAGFHRLPLPGQPVRELLLEI
                     DNGDNPPLDITQVFGVVSVPRLIYKGDAQKEYRLLIGNPDVQAPSYDLASLRNVLLDY
                     PARLVVLGSLMANPNFRPPSNYFQEGRMNLVLLGVLLLCVVVLLALSLRLLRNPHPEK
                     PPPIDTDGQNPPPSPPAA"
     gene            complement(26285..27601)
                     /locus_tag="PKL17_07585"
     CDS             complement(26285..27601)
                     /locus_tag="PKL17_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNK44627.1"
                     /translation="MNSEKSDVQAEATLVTIGDELCRGEIVDSNAAFLGSELTGLGVY
                     VRQRIGCNDRLPHIVECLKTAAKTSQFVVASGGLGPTSDDLTVDAVSSLLGVSPVSEP
                     DHEARMRQRFTERKFTITPNNLRQVRIPAGAFVLANRTGLAPGFAVQIGGARCFFMPG
                     VPREMKPMFSEQVVPHIRTNLPPRTVIRSVYRSLGLGESHVDHRLQDLLSSTAELRHA
                     ETVEVTIHYRLAFPEVLVTLMLSGSDGNQVAQAQAVLDAEVRRRLGHALYGTGDADLA
                     MVVGEALKSRGETLATAESCTGGMIGQTITAIAGSSAYYLGGVVAYANQVKQAVLGVS
                     DATLRDHGAVSEACVRELSEGVRKLLGATYGIAVSGVAGPSGGTPEKPVGTVWIAAAG
                     PAQTICRQILWPGDREQIRKIATAAALNLLHKLMFPARLSDPALRI"
     gene            27860..>28344
                     /locus_tag="PKL17_07590"
     CDS             27860..>28344
                     /locus_tag="PKL17_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNK44628.1"
                     /translation="MKRQQNEDYYDTFEEEKLFIVADGMGGHAKGEVASKLATETVKE
                     FFKRTRDEEATWPYKMDRQLSYVENRLVCGIKLANWKVHESATKDPMCKGMGTTMVTC
                     MISGDKSYFSHVGDSRGYRIRDGVIKQMTRDHSLLEDYKEAKPDMTEEEQKNFPHKNV
                     IT"
BASE COUNT         5536 a         8858 c         8618 g         5332 t
ORIGIN      
        1 cggtgcggtt gcggttgacg cgcggaccct tttcttcctg ccacatccga ccgccgcccg
       61 cgccgcagca gaagccgcgt tcttttttgc ggcccagttc gatgacgcca gtggcgaccg
      121 catcaatgac ggtgcgcggc gcatcgtaga caccgttcca gcggcccagg tagcacgagt
      181 cgtggaacgt gaccttctgg ttcattttgg tgtcgggctg tagcttgccg ctcgacagaa
      241 gatgggccag cagttcggag tggtggagca cttcatagtt tccaccaaac tgcgggtatt
      301 cgttcttcag ggtgtggaag cagtgcgggc agctcgtgac gatgcgcttc accttggctt
      361 cgttgaaagc atcgacgttg gcctgggctt gctcctggaa gacaaactca ttgcccgcgc
      421 gccgcgccgg atcgcccgag cagccttccg acttgccgag cacagcaaag cttgtacccg
      481 ccgcgtgcag gacttcgacg agtgcgcgca tcgacttctt gatgcggtcg tcgaacgccg
      541 ccgcgcaacc aacaaacagc aggtattcga agtccggctt ttcttcgacc gtcggaaccg
      601 gaacgtccag cccttccgcc cattccatgc gctggtcggc attcacaccc caggggttgc
      661 cctggcgttc gaggttttgg aacgtgcgct gaagttccgt cggcatgcgg ccttcggtca
      721 gcaccaggtg ggtcttcatc tgctgaatct tcagcggatg gtcaataaac accgggcaga
      781 cttcctggca ggcaccgcac gtcgtgcacg accacagcgt ttcgtccttg atgcggcccc
      841 cgaccagcgg tggcagctcg tcgcacttgg cttgcaggtc ggcaatctgc tttttgaacg
      901 cctcgggcgc tttttcaagc tgggcttcgg cttcgctctt gtccttggcg gtgacgccag
      961 cctgcgcttt gagcgcatcg agctgcatcg ccagggcacc acgttccttc atttcgtctt
     1021 tcaggtcgtg gatcaggtgc atcggcgaca gcggcttgtc cgtgttgtag gccgggcagt
     1081 agttcgagca gcgcgcacac tcggtacagg cgtactggtc gagcagggac ttttgcgaaa
     1141 actgctcaat tttgccgacg ccccagtcgt tttcatcgtc gaggttcagc ttgggcatga
     1201 cgcccttttg gtcgagcttg cggaagtaga tgttcggcag cgaacccagg atgtggatgt
     1261 gcttcgagta cggcaggtag ttgaggaaga acagcagaat gccggcgtga atccagcgcg
     1321 acacctcgct cacaacgtga gcaccgccca ttcccatcgt ggccttgcca atcgcatccg
     1381 acagcggacc aaagctttcc ggattctgcg ccgacgcata gtggaagccg tgaaagccaa
     1441 agtggctcac catcagcatg ccaatctgcg ccaagatgaa cgcggcgtcg cccgacagcg
     1501 ggatgagccg aggcttgacc accacccgtc ggaagtaggc gtagaagatc atgcacagca
     1561 cgatcccgtt gaagatgtcg atgctccacg acagcgccgg atagaccggg cccaggatca
     1621 tgcggaagct aaagcccggc acgatgtccc acaaaagccc ttggagcata aactcgacgg
     1681 ttccgatcgt gatgatcatg aagccgtaga agatcagcag gtggtgcttg gatgtggccc
     1741 ctgccggcgt ggcatacgac atcggctcag cgactttttt ctggccgaag aagtaaatca
     1801 gaaccgacgc gatgcgggtg gcgatgcggt cggtgagctt gagatcgttt tctttgcccc
     1861 gcgccatgat gcggtacagc cgcagcactg aacgcaaaaa cactgcgctc gcgacgagca
     1921 gcaggaccag aaacactatc gagctaacca tggattcatc ccttcccaag cagttccggc
     1981 tttggtgacc tgccttatat cacagtctgg gcgatcacga ccgacgatgg atcgaagctg
     2041 ccttataatg gcacggtgcc tcgtgcaact ctccgcgttc gcgaccttcc tgaccccctg
     2101 accttcgtga ttcatcctgg gaccgcactg ctgataggac ggatgcccga tccggaaagg
     2161 ctgcggcgcg aacaccccga cctcgccccg gtcttgaggg accgccgcgt gctgacaacc
     2221 cggattgatt cgctgcgggt gtcggcaaac cacctgctgc tcgtctgcac ggacgaaggc
     2281 gaagtgtcgc tttacgaaac ccacagccgc aatggcacgt cgctgcgcct ggccgcgggt
     2341 cagccggtac aactgcaagg tgatgtggcg ctcgatctgg cgccacgcgg cgggacgacg
     2401 acaggcattg gtgaaccacg tccagccgac tggagcgatg agggcgactt tggatcatcg
     2461 gttctggaag ccatcaacga gtggcttagg ccacagactg tcgacctgcg cgccgagctg
     2521 cgagcatccg gcaaacagga cagcgatggc tggacgttcc acctggccga cgatcatgag
     2581 ctgcggattg gatcgctgag cagcggcacg tatgacccgc aggtcggcgt cgtgctggac
     2641 cgcattcgcg tgtacgttca cgaacaaaac ctccgcttcg agcagttcaa gcgccgtgtc
     2701 gatgggatga ttgtttcgtc gcccatcatg cgcgacgtgc tgcggagggt gtccgaagcc
     2761 gcagaacaag gtcgacgcac ggtgctgctc ggtccaaccg gcgcgggcaa agagtggctg
     2821 gcgcgctgtt atcaccgaaa ctcgccgcgc gcgacggggc catttgctgc acttaactgt
     2881 gcgctgctcg atcgcgacct tttgtttgcg cagctctttg gtgccaagcg cggttcgttt
     2941 accggcgcag tcaacgacat tccgggcatg gtggaagcgg cccacgaagg gacgctgttt
     3001 ctcgatgaac tgggggaaat gagccttgat gtccaaaaag ccctgctgcg ctttctggac
     3061 agccgcggcg aataccagcg actgggcgat ccgaagccgc gccgcgccga tgtgcagctt
     3121 gtctgcgcga gcaacgccaa gctcgatgaa aatgactttc ggatgaagca cttccgcgac
     3181 gacctgtggt atcggctgtc gtcggcggtc atccgtgttc cacccctgcg cgagcggcca
     3241 gaagacgtgc tcgcgtttct gcagctcaaa acactgcgag ggagccagct tcgggtggtt
     3301 gacgcgctgt ccgccgtggc gctcaagagc gttttatcgg acccgctgcc tggcaacttc
     3361 cgcgacctgg aaaacttcgt cgatcgactg ccgctggtcc aaaagcctgg cgaaattgat
     3421 gtcgcgacgg tggaatcggc actgcgcgaa ggacgcggca cgccaagcag ttcgtccgcc
     3481 gtgccgcaag agggctttca ggtcaaagac agcggtcggt tcaagcgatt ccggcaaccg
     3541 caggatcgtc cgccagacaa tcgcaacctg gactgggacg gcatgatgtc ggcggcggtg
     3601 gcggcgtttc tggccgacca tggggacagc accaccagct ggggcgagct ccagtcgctg
     3661 gtcgaaaaat acgtcaagcc ggtctttgtc gcgtacacca ccgagatggc atacgtcgac
     3721 gagctgagca aaaacgtgaa ctactccgca ctggcccgca agctcaacat tgcggacggc
     3781 agcacggtca aaatgcatct gagtcgctac gtggagcgat ttcgccagaa gaagccgggc
     3841 ccatagcgcc gccaggacgc tgtccccaca gtcggtaaag gggcttgggc agcaagtcgt
     3901 gaagtcgcac aagcggccag gccaaggcgt agccggaaaa cagcagcagc accggaatcg
     3961 cttcgacaaa gtagcgctga agcggaatcg gcagcaaaaa ggcaaacaca agcgagcgca
     4021 caagcggaat gagcagcagc gccgccgccc agcgccgctt gccagggtca agcagcgcga
     4081 tgcacgcgcc aaaccctgac agaagcagca gcgccgagca gacattgtcc caaaaaggac
     4141 gctgtgtcgg cagtcgcaaa agccgcgagc ggaggctcag ttcatacgac ggcatcggcg
     4201 accacagcgt ccggattcgg tacagcggca agatcaccag cgtgcgaaac ggatgtttgc
     4261 gcagccgctc ggctgccagc gcgtaaaaac cacggtcgac atccgggctt tcgccttccc
     4321 gacagtagcg attgaacaga tcgaacagcg cctgccgttc cgcatcatcg tcgtacatcg
     4381 tcggcaggat gatgttgggc cgtgtcggcg gtagctgtcc gtgattggca gcgacaaaca
     4441 agcagtagtt ttcgccatca atccgtcctg tcgcccacgt ctgtgcccag cgcatcatcc
     4501 caagccgcat cggcacgccc tgggtgtcaa tccacggcga tggacccagg tgcagttcac
     4561 caaacgtcag cagactgcgc agcggccacg gggaatacac catcatcgac agcgcaaaac
     4621 aggccagtac ccagcgaagt cggtgcttga tcgtgcgacc ggccatccac agaacaacca
     4681 gcagcgccgg caaggccagc acggaatcaa gcctcaccca cagcgcaagt cccagggaaa
     4741 gccccgccac cagcgcaaag aaaaacggtc gcgcagcccg acgggttgcc aatgcagagc
     4801 agcaagccag cgtcagaagc caagtcgtca gcgattcgca caagccgtgc gaggccagca
     4861 taaacagagg tggcataaaa atcgtcgaag caaagccaca cacctgcgca agccgtccca
     4921 tccccaaaag ccacgccatg acggcgacca ggccggcagt tccgacatcg agcacggcgt
     4981 tggcgcgcgt cgcaatcacc agatgctgcg gcagatccac gggggtagac ggcgacagca
     5041 tcgccaaaaa cgccggatac cctggcgagc gggtccaggt gagcggctgc ggcggtggtc
     5101 cgtaagcaaa acgatgggcg tggcgaagct gctgggcaag ctggtgatag tgaatcccat
     5161 cgcctgcgga ggcacgaaac tcggcgacca atgacaaccg aaagacgagc gccagtgcga
     5221 ccagggcaag ccatggaacg actaccgtcc accggggcac gaggcgacga ctcatgcggg
     5281 caagaaaacc acgccgaacc cgagcttggg aagactagaa cgagccagaa agagacaaag
     5341 caggggtcgc ggaggtcggc tgcatggccg tctgtgacga cgccggcttc tgggcatcca
     5401 gcagaaacag cgccagcgag ccgccgagcg aaagggtgcc aatggtcagc agcgctgccc
     5461 cccctgcccg tccatcaaat gggcaagtca tcgtcggatc gctgctacaa gccatcggtc
     5521 gaccattgag ccccagcagc gtcgcaccag ccgccgccga cacaatcccc agccccagcg
     5581 tcgtccattt caggatggcg cgcgaacgag acggcgcaac gaccggcagc ggaatcagcg
     5641 gcctatcaag cttgagctcg cggttgtctt ccaccagcac ctgcgcgaca agcggatagt
     5701 agccggactt ttccagcacc agctcatagg ggccgatgaa cgcatcgcga cgcaggggcg
     5761 tgctgccgat gttgcggggg cccagcttca ccgtggctcc cgccggttcc gatgaaacca
     5821 acagcgttcc acgcggcttg ttggctgcgg tcttgagcaa gtccccgacc atgtcggcaa
     5881 tccggctcat cagcttgtta cacgccgagg ccacgcagct atcggttgcg gtggcggcaa
     5941 tcgcgccgac ttccacgtcc agcaccgatg cggaatagcg atacgcgacg accggacgct
     6001 gcgagctgcc cgactggagt tcaggcacgc taagcgccgc cgctcccagc tggaattcca
     6061 agcgcagcgc ataggttgcg ttcgactgca tggccagttt ttcctggcag cttggctggt
     6121 ccaggcagcc ttccagctcg cgattttgcg ccatcacact ggcttgggta tcaggggaca
     6181 ccaacaccgc ccgctgacgc ccgactgcct cgctcacggt cttgcgcagg ttggtcaaaa
     6241 agcccggttc cagcgactgc ggttcaatca gcagcaaaac accaggggtc agcgtggtga
     6301 ctgcgagcgg tggtttcagg gtaaagcgca cctcagcagc ccggcgcggc aaaagtgtca
     6361 ccgtggcttc gactttgcgc ccttctttgg tcagcaccac ctgatgtttg cccgccgata
     6421 gctgaacggg cgtcgccagc ggcagcgaac caaccagtcg atcgtcaacg gtcaccaagc
     6481 cgccgggcac acccgcgacc gtcagatcgg cgccgacttc gagcggctgc tgtgccactt
     6541 gggttagctc ggcgcgaacc tccgcttcgg catcgtcacc gacttgctca atgtagcgac
     6601 gatacaagtc gatggctgac cgggttcgac cttccagaag tgccgcccgc ccaagcaggt
     6661 acaaaatccc cggcttcggc caatagcgat aggcatcttc gagcaccaag atcgcgcggg
     6721 caggatcgtt attttccagt gcctgtcgac cttggtcgat catcgccgtc gacttgcgat
     6781 cgccggccag actacgtagc gccggcaagt ccgcccaggc gagctgggaa acgaacagca
     6841 ccgcgatgcc tgcaagaaaa cgccacatcg tgtttgtccc tgattcgatc ctgatttgtc
     6901 gacgatccac catcatggcg agctatcaat ccggccactt gcgcacagct cgacgcgcgc
     6961 ctggcccccg gcgcgaacac gaacaggtcg accgccatcg acaaggtgtt cgccgggacg
     7021 aacccactgc atgatcgtcg tacactgccc atcgacttct ttgctgatga tgagccggcc
     7081 aagatacggc tgaagccgcg acgatgcacc gagcgcagcc gtcgtatgtt cgggtctgcg
     7141 ttccggcagc cgaccgagcg cttcatactc cgtcatcgcc tcgaggtgac tgccgttttc
     7201 ttcgtggatg cgcgcctgct ggtacgtaag ctcggcaaac atggcgcgac tcagcttgcc
     7261 gcgactgccg agcgcgcggg ccctgtcgag cagtttctgc gccgctgtgg tttcgtgccg
     7321 tgacagcgct tcggcggcct cgatcacaaa ctgatcaccg agcaggatcg ggattcgcac
     7381 cgacgcagtg ctgccggcca cgatctctac atcgatcgca ccgcccgcat agcgcacttt
     7441 gtgtttaccg gcggggaccg tcactgcggc catgcccgga cccagaagct gcgccgcttg
     7501 atcatcgact tggaaacgaa ctttgtcgtc gccctcgatg cgcaaaagac cggtctgcac
     7561 cgtcggtcct gcatctaccg tggtcagatc aggaggaacc cccaagtcgc ccggtggttc
     7621 ctcctgcgct tcacggctct gggacagcaa gctggacacc cgcgtccgca ccgccatttc
     7681 gggatcggca agcaaaccaa tcatcagcat caaaagcggt tcgcttgggc tggctttgta
     7741 gaagtcatag ctgacttcgc cgacgcgacg acgcacttcc ggtgaagaat ccagccgcgc
     7801 cttttgcaaa agtggcagcg cttcgggcag cggcaggtcc gcgatgatgt ccagcaccgc
     7861 cagactggtg ggcatgtcgc cacgacccaa cagctcgcta aacgacgccg gtggcgacac
     7921 cttttcgccg gcctttttga gcagaccgta tgccaccaag ctgtcgacac caccgctggc
     7981 caatacttcg tgcagcaccg ggagcgcctc tgcgaccttg tgaccgatga gctttcgcgc
     8041 cgccgcaaaa cgtaccgaca aggcctggtc cgcgagagcg tgccgaagca gctcatcgcc
     8101 cgcaggcagc atctccacaa cgagcttgcg ggtcagcgga tcgggcgctt gcagtccttc
     8161 tcgcagcctt tcccggtgcg catgttcgcc aagctgtagc agtgtccctg ccgccgtaag
     8221 ctggtcttcg gtgttgcccg tttcgacaag ctgccgcagc cgcaggatca gctcatcgtc
     8281 gtgggcattc gctcctcgtg acaaaagatg cgcggcgacc cgcgacacac tcatcatgcc
     8341 ggcacgacgg acatcgggtt cagcatcgcc gagcgcctgt cgcagtgcca aaagggccga
     8401 acggacccgc ttgcgaccca gtgcagccgc cgcgccacga cggaccgact gctgcggatc
     8461 gctcagcgct ttggtcagaa gcggcaccga gtccgcattg tcgatgtcgc caagaaccag
     8521 cgttgcggac tcgcgaacca accagtcgtc gtggcccagc gcagcttgcg cccagcccag
     8581 gctctgcttg gcgatctgct cgggatctcc acctgcaatc tggacgatcg atcccgccgc
     8641 cgcttggcga agcagttggg acgccttggc ttcgtcgagc actttgagaa gctgctgcgc
     8701 gccttgtcgt ctgccacacg cggaaagacc atctatcgcc acaagacgtg atggttccgg
     8761 ataagaagca gacgttgcaa cctggaccat ccggacaaag cccaaggaat ccccgacggt
     8821 cgccagcatc agcgccgcca gaagctgcgt cggtccttcc ttttgggtcg cttgccgaag
     8881 cagttctttg ccactcggtt ccccgacgcg cgccagaccc gccgcagcag aaacccgacg
     8941 caagctgaat tcatcgccgg ccagcgcttc gcggagtcga gccaccgcct gtgcatcacc
     9001 ggtctgcgcc agtcgtgaca gcacgcgcag ttggacatct tcaggcagtc cgggcttgtc
     9061 gacggcatcg cgcaacaccg gacgggccgt ggccgcatcc ccaccacttt cgacgagcag
     9121 aagcgccgcc cgcagacgga ttctttcatc gctgctttgc aaaagctctc gcagcaccgc
     9181 ccgaccgcgc tgactttgca gcttggcgag tgccccggcg acggcaactt tcagaccaat
     9241 gtttggattg ctatcgagca gctgcagcag cgccggctgg gcatcaagcg cccccagcgt
     9301 tccgagcgcc gacaccgcct cgacttgcac cagcggctcg ggatctttca gcagcgacac
     9361 gatgctggtc cactgggcac tgtcgcggct gtgacccaaa gcgcgcaccg cttgcgcccg
     9421 caactgcgga tcgggtgccc gcagaccgac gtacagcacc gacagggctc gcgcccgcgc
     9481 cgcatttaca tcgatcgcca ggtcgttggc atcgaccacc aagtcggtgg caggccgtgg
     9541 ccggaagtgt tcttcataga caaatacgcc cgcggcaatc gcacaggcaa gaaacagccc
     9601 ggtcagcacc caaacgattg gactgcgacg cggcgccggc accacagcga cttgcgctgt
     9661 cgtcatcggc ggcggcgcaa gttctcgcac cacatcggcc atcgccggac gctgttccga
     9721 cttgggtgcc agcatggcgt gcacgtattc cgccagcttc ggactgaccg tcggcgccac
     9781 ttctttgagc ggtcgcggcg gcttggcaaa cgccatcagc gacgtcttcg gaaacggcgg
     9841 catcccagcg agcatttcgt acagcatgac gccgagtgca aacacgtccg ccttgccgtc
     9901 caccttcgcg gcgccgccgt actgctctgg agccatgtac tttggcgttc ccatcgtcgt
     9961 gccgacctgc gtttcgaagt cgggagactc cggatcggca gcaatcgacg tcgccacttt
    10021 cgcaatgcca aagtcgagca ccttgacccg ccgctcgtcg ggtttcaagg ggtccggcac
    10081 cagcatgacg ttttcaggct tgaggtctcg gtggatgatg cccttttggt gggcggcgag
    10141 cagggcttgt cctatctgta gcgcgaggtc tttggccaat tcctcgggga gcgcgccgtc
    10201 tcgacggaga aggccgcgca gtgactcgcc ctcgaggaac tccatgatga tgtaaagcgt
    10261 cccgtccggc agttgtccga agtcgaagac ttcgacaaga ccaggatggc gcagcatgtt
    10321 gacggcacgc gcttcattga gaaaccgcgc cgcatagtcg gcagtccccg ccagcggacc
    10381 gtgcagaatc ttgatggctg cacgtcggcc aatttgcttg tggaccgccg aatagaccga
    10441 tcccatcccg ccttgaccaa tcttggccgt aacacggtag ttgccgacga gctgtcccga
    10501 ccgcaacgcc acctgccccg ccacaccggg ttccgctgac gtcgcagcag ttccgccatc
    10561 gcccgtcttg gccgacgtcg aaacgccgcc gctttgggta tattccgccg atatttcgac
    10621 ggctggcgac atcgcatccg atgaagcaac cggcggcaca gcaggtgccg aacgcccagc
    10681 ctgcaccgtg ggcggaacca tggtctgagc aaagccaatg ctgtcccctt tggggcggtc
    10741 tccgggcagg gtcagtccgt ctgacaaact atcgggcttg gcatctttcg cagcggcagt
    10801 cgaagacgcg gaagcggcgg gcaaggtctg cggtttgaca tcggccttgg cggcggtggc
    10861 aacctgccct gggatggtca gcgagtcgga cagcacatcg ggcgttgctg gcggtgacac
    10921 gttgcgtggc agcgaacggc tcgatggtgg caagctcgat gatgaagccg gctggacggg
    10981 cgacggtgca tggacgggcg gtggcaggct gctgctcgac gctggttgct gtgacgaaag
    11041 ctgctgaagc tgggctgcga cttcgcccat ggtcggtcgc gctttcgggt cggtggccaa
    11101 catacggtcc aaaagcaccg ccaaaggccg ctgccacgac ggcaaaatct gcggcaagct
    11161 ggtgacttgg ctggccccgg attcatccag cgacgcctgg ctcggcagcc gacccgcggc
    11221 cagttcatac agcagcaccc cgagcgcaaa aacatccgat gctgtcgtcc ctagctcgcc
    11281 attgcgctgc tcgggggcca gatagacgcg ctcgtcgctt ccttttacgt cgctgatcac
    11341 accgccggga agcagatcga gcagcgactg cgcaatcccg aggtccatca gcttgatctg
    11401 ttcaccctcg ccagcgctcg tcgatacaac cactttgctc ggggacagcc gaagatgaca
    11461 aagggatgcg gagtgcgcca gcgtaaggca ggtcgcaagc tgacggccaa tccgcagcgc
    11521 cttgttgggc agcggcaagc ccatcgaacg acgccgctgc tcctgaagcg gttcgccact
    11581 gacccattcg ctggccaaaa acaccacacc gccgggcaga tttccgtact ccagcagggc
    11641 cggcaggacc ggatcgggcc gtgcacctgg ctcgacttgg ctttggccgc tgatcagctt
    11701 gctgacggac ttgcgcagcc ctttgagccg ggacagcgcc ctgccgtctt tgttgagctg
    11761 agcgggcagt acccgcatca ggacttttgc ttgggtctgc gcataggtgc cttcatgcag
    11821 tgcgcccagc ggcccatctg cgagctttgc accgacgctg tagctcttcg aatccatcac
    11881 cgcaacgacc ccatgccccg cacgcggagc ccgcccctag accgcagtga gctaagaaat
    11941 gtatgagcca ccaccatgaa tttaaccgat ttaatccgta catgtttttt cttctgcgtg
    12001 tgatctcggc ccagcctgcg caaaagcgac tgtcaaagcc ccccttgtat gttggcgggg
    12061 tgataaaatg ggcccgcctt tatgagtcgg tctagcagaa gcgtacctcc aggccctggt
    12121 gacggcgacg gcccacccag caagcacgag cttggcctgg atgtgaccgg caaacgctct
    12181 gatctgcctc gcgataccga tcctgatcca aatgccaaag tgacgctgaa cccggatctg
    12241 gcttcggcga ccttgcgcga accaggtctc gcggtggaaa cgcccccggt aagcagccgt
    12301 ccgtcatccg atctggcagc caccagcgaa cagcttgcgg atgagtcagc gctcttgcgc
    12361 aagcaaatga cgcgcagcaa gcccaccatc gaagagctgc cgcgcggcgc aacgatcggt
    12421 cgctacatgg tgctgtcgtg cctgggatcg ggcggaatgg gtgtggtgta tgcggcctac
    12481 gatccggaat tagatcgaaa ggttgcgatc aagctgctca agtcgagcag cagcggcagc
    12541 agcagccggg tgcgtttttt gcgcgaagcc caggcgatgg cgcggctgtc ccatcccaac
    12601 gtgatcaatg tctttgacgt cggaaccatc gaaaaccaag tcttcatcgc gatggaattt
    12661 gtcgatggtg ggacgctcaa tcactggctg accgacaaac cacgcacgca gcgcgagatc
    12721 atcgacattt acaccctggc gggacgaggt cttcaggccg cccatgcagc cggtctggta
    12781 catcgcgatt tcaagccgga caacgtgctg atcagtcgcg aaggccaggt gcgagtcatg
    12841 gactttggcc tggctcgatc gattgggcag tcggaagacg acacaaccct gccgcgcgag
    12901 cttacgtcgt cgtcgtccaa tcaagcgctg aacattcgac tgacccatac cggcgccatg
    12961 atgggcacgc cgcgttacat ggcacccgag cagtacgaag gacaggcgac cgaccagcgc
    13021 accgatcagt tcagcttctg cgtcgcgctg tacgaagcgc tgtatggagt gccgccgttt
    13081 gttggtgatg acataaatac gctcgggttt aacgtcgtgc gcgggcgggt ggcgtcccca
    13141 ccgccagaca ccaaagtgcc gacgtggatt cgccatgtgc tgctgcgcgg gctccaggtc
    13201 aaacccgaag atcgctttcc gtcgatggaa gagctgatcc tggcgctcaa tccacagacg
    13261 aagcgactgc ctagaccatg gcttgttacg tttgtgctgc tgtcgctggt gctggtcggc
    13321 aacctgattt accaggtgcg agaacagcga cgggcgggca tgtgcggtgg ttcggaagaa
    13381 aagctgcgtc cgatttggtc gagtcagcgc aaaagcgagc ttgaacaagc acttttgtcg
    13441 tcgaaacagt cgtatgcgca agatgtctgg cagcgggtcg agcagacgct ggaccgctac
    13501 gcttcagact ggtcggccat gcgcaccgag gcgtgtctac tcaccctgcg cggccagcag
    13561 acgcagcaga tcctcgatct gcggatgcgc tgtttggatg atcgactggc ggagctgcgt
    13621 gctctgacgg cgattttgca gaaagccgat ccgacggtgt tgcaggaagc ggcgtcggca
    13681 acgcacgaac tgtcccccat atctggttgc gccaacatcg aagccctgag cgcccccatt
    13741 ccgctgccgg acaacccggt cactcgcaag cgcgtcgatg cgctgcgtca ggaaatggcg
    13801 gaagtgcggg cgatcgagcg ctttggcaaa tacagcgagg cgctggtgcg ggcggaaaag
    13861 ctggcgcatg actccgtgca gtcgcgctat aagccgctcg cggcggaagc caagtacctg
    13921 ctgggtgaaa tccagatgcg cggcggtctg ggcgcacaag ccgagcagtc gctggtgtcc
    13981 gccgtgatgg cagcgatcga ttcgcgacac ctgcgggtga tggcggaaag cgcactgctt
    14041 ttgaccgaaa cgaccggttt tcggatcaag cagttccgca aagccgaagt ttggtcgcag
    14101 attgccgagg cgatgctcga agaaatcagc gatcccgagc cgctgcgcgc cagtctgtac
    14161 ctggcaaact gcatggtcgc ctcgcaggaa aatcactatg aacgagccgt cgaccagtgc
    14221 caaaaagcgc tgagcctgcg ggaaaaactg ttcggccacg aaggtcccga agttgccgaa
    14281 gcgctgaacg tgctggcggg cgtctaccgt cggtcagagc ggctcgcgca agcgatcgac
    14341 ctttataacc aagcgctgcg gattgaaacc gcccagcttg gatcgctgca ccccaacctg
    14401 gtgtcgacgc tgcgtggcct tggaatcgtg gcccgtgatc agctccggta tgacgaagcg
    14461 ctgtcgtatt tccagcgggc gctgtcgatt caggaaaagt cgctcggcgc ggacaacatt
    14521 cgggtcagcg actaccacat tcagcttgtg cgcacgctgt tggcgatgaa ccggctcgac
    14581 gaagccgaac agcacgcgga aaaagcggtc agcattcgcc gcaaagccga tgcgcgcgtc
    14641 gacgtcgaac gactgacgga agcgatttac tttttgggtg aagtcaagtc ggcccgcggg
    14701 cagctccatg aatcgctgaa gcttcacgaa gaagcgatgc gactgcgcga acagcagcgc
    14761 ggcgaccgac gagaagagat gctggcattt agcctgacca gcgccgggga agtgctgttt
    14821 cagctcggcc aagcggcggc ggcgcggccc tatctggaac gggcgctgaa cttccgcgaa
    14881 acgcagccag cggatacgcg acgtgcagag cggtccgcgc tgggacgcac gcagtttgcg
    14941 atggcgcaag tgctgtgggc gctgggtgat ctggaacagc agaaacaagc catcgagctg
    15001 gcccaagccg cggcccgcga atacgaagcg tatggcagcc ggcccggcaa catcttgccg
    15061 aagatcacaa cttggctcga catgcatcct ctgccgacga ctcggaaaaa ccatgcgcgc
    15121 tgatcttcct tcgcttttgc cgctgatctg tccttgctgc cgccatctgt cggcgcgcgg
    15181 acgagagctg tattccctgt cggtgggcga agtgctgcgc tcgactggcg gtgaagacga
    15241 cggcaccccg gaagacatcg aagaaggcat cctggtctgc gacaatccgt cgtgtcagcg
    15301 tcgctatcca atcatcgctg gcatccccgt ggtgctgccg gacttgactg gctttgcatc
    15361 gaaacagctg ctggcgctga gctgttttca tgaacccgaa acgctggcga cgtttgccgc
    15421 gcaagggcct gacgatgccg cactgccccg gatgctcgag tatttgtcga tctatctgga
    15481 tgcacatttt ggcgactact cgattcccgc ccccgacggt cctgaacccg gctgcggggg
    15541 cacccagctt tggcacgcgc tgtctggttg caatgccgct cccgtcgagc gagccatcga
    15601 acttggctgc ggcgtcggtc gcggtctgca cattctggcc gaaggcgccg aactaaccgt
    15661 cggtgtcgag ctgaactttg ccgcactttt gtacgcacgc tggattttgc gcggcgaaac
    15721 ggtgcgttat gcgcgcagaa cgagcggtcg gcactatcag ccagcgagca tccacgcacc
    15781 agcgcggagc gcatcggctg tgcagcttgt ctgtggggat gcgctcgatc caccgctggc
    15841 gcccgatagc tttcagcggg tggcatcgct caacgtgatg gatgcggtgc agtcggccca
    15901 tcagcaccta tcggtcgtcg atggactgtg cgtgcctggt ggcgaagtgg tgctgtcgtc
    15961 cccgttcaac tggcagtcag gcattgttgc ggaagatgga cggctcgatg ctgccgaccc
    16021 ggcgcagctg ctccacgaca tgctgcaaag tggccgcggg cttctgtccg gttatgccat
    16081 cagcgaaagt cgtgagctgc gctggcggct ccgtcgcgat ggtcgcagtg cggtcagcta
    16141 ctttacccac tatctgcgcg ctcacaaacg cggacagctt ccgcgcccga cgtaaacgag
    16201 gcaagccatg gttgtttttg gactgagtca tattgacgtg cccgtcgtca acctggaccg
    16261 ggctgagcag tggtggtgtg aacgtctggg ctttcgggtc aagacgcgca gcgacggatt
    16321 tttggacatc gacgtcaaca cctgcattgt ccgcttgtcc aaagttcgcc gcgtcgaaca
    16381 tccggtgacg ctgcggatcc aggtcgacca cgtcgaatcg gcgctggcag acatcaaaca
    16441 ggtcggcggg cgggtccagt acgaaccgct gcgcaccgca gaacaagagc tggtttcggc
    16501 ggtgctcgat cccgacggac acacgctgct tttgtggcgc ccgctcagcg aagacgagta
    16561 cgaaaaaatc cctgagctgc caaaagaaat gacctggcgg cccgacgcgg aggagctgct
    16621 gcaaacgctg ctgcggagtg taccgtcgct gtttcgagcg attgcgcgct ggcgggtggc
    16681 caagaacgcc gaatacctcg ccgagtcgac caaggtggtc caccgcgagc aagtgattcg
    16741 cgcgttcatt ttgtcgagtg ccaagatcac gcgctatcgc aaccgacagc cgctgatcga
    16801 tgcgggaatc gacgtcagcc agtacgaagc cgactttgct gccgaggaac gactgtagtt
    16861 cttacgctgc gaaaccagca gcgcgacgac tacttcgggt cgcggtgcaa aatggcgccg
    16921 ccctgccctg cgaaccaata cttgccgctt gggtctttgg ccaccgcgct gagcgccccg
    16981 gaagtcccgc tttcaacggc acgccaagcc gtgcggtcga agcgaaacat cttgccgctt
    17041 tgtccgacga tgtacacttc gccgcctggc tgaccggcga tgccgagcaa ggtgatcggg
    17101 ttgcgctcgt cgcccacgaa gtcatattcg tagctgctgc cgttgtaacg gatgatcacg
    17161 ccgttgtcgc cgacgatcca gacctcgcta ggagaagcgg cccagacggc gcgcagcgta
    17221 tcgccagtgc gtgaccagga ccgctgccac tcgccatcga ccagatgcaa gatgacgcct
    17281 tgctcaccaa cggcccagac atcacttcct gacgacccgc cgaccgcatg cagcgcttgg
    17341 gtgagcccgg tatcagtccg agcaaaaccc tgaccgtcgt agtgcagcaa cagtccggcg
    17401 cggcccacca tccagatgtc tttggaccca aagccgtaga caccgcgaaa atccgtcgag
    17461 acgccgcttg tgacaaggtc aatgcgctgg ccgccgtagt gaagcaccgt gccaaagtca
    17521 ccgacgatcc agacatcgtc gcttttggcc gcatagaccc cgcgcagtgg atcggtggtg
    17581 gtgctgggaa ctcggtcaaa aaggccgcca tgcgcatgaa gcacgcggcc cgcatcgccg
    17641 accaaccaca aatcggacgc ttcgcttgcg cttacggcaa acaagttggt gatgtctccg
    17701 tcgctcgcga gcttccaagc gagtccatcg aagcgcagcg tcgtaccgcc ttcccccacg
    17761 gcaagcccaa gctgggtcga aaacaacgca ccagaaagca gccgattcgt cgtcaaaggc
    17821 ggcagcgttt cccagcgccc cttggccagc cgcagcagca tgccaagctc tccgacagca
    17881 aacacatcgc cttgcggact gccaaagacg gagtaaaggc gcggtccgct ggtggcaaaa
    17941 tagcgaatcc acgtctgtcc gctgctgtgc aaaatgacgc cggtccggct tgtgtagtcg
    18001 tagccgacgg cccacaggtc gcttggcgat gcgccccaaa tgccgacgac gagcagcgtc
    18061 gagcccatcg gcaccgcgtt ccatttcagt ccatcgtagt gaaacgactg actgccatag
    18121 cccatcgccc agacatcgcg cggcccagtg ccaaagaccg ccgaaaacgc catcgtggtg
    18181 ccaatcgact gctggctcca gatcgcgccg tcatagtgga tgagcgcacc gttttctccg
    18241 acggcccaga tgtcactgcc actgctgccc cagatgctgc gcagcgattc gatcgtcgga
    18301 cttgcgacgg aagcccactg ctttccgtcg tagcgcagaa ttgcaccgtg ttctccgaca
    18361 gcccacgccc ggtcggtcgc actgccccac atgccgttta gcgtctcgct gctcacgacg
    18421 gcggtcgaaa cccaactgat cccatcgaaa tgaagaatcg aaccggcgtc accgaccgcc
    18481 cagatgtcac gcggtgacgg accccacagt ccgcgcagcg tctgcccttg cggtgatgga
    18541 cgatcccaac agaccgcatc gggcgaacat acctgcgctt tgacgacggc gacttcgaca
    18601 tcggtgggct tgctgacggt gacttggcaa ggctcggttc cgacgcaagg caggcgaaaa
    18661 ccgagcagat gttcgcctgt ggctggatcg acgtgaagcg aaagcgagct gcctttgcga
    18721 atcggacgga cgcacgaacc tgtgcaatcc agctcgccgc ccatcgatac aacgcggctc
    18781 tgcgcggcgt cgggcagaag cacgcgcact gggcaggtgg caaacggctg tttggtcaga
    18841 aatacatcga cggtggcttg gccgtttttg tcgatgcgga cggcgccacg gccttgcgcg
    18901 cgggtacagg cgtcaccatc gagcgcttcg atcagcactt caagctgacc ttggctgcta
    18961 gacggcaggg tcaggtgaaa atagggcgga tttgaaggga accgctgcga atcaacgacg
    19021 agcacgccat ctagacgcgc ggacgccaca aatccactgg tttcggcatc cgtgccgagc
    19081 accgtcacca gtacgaactg ctcgccgcca cagcttgcca gctgcgaaag cagaagcagc
    19141 agcgacagca gccacagccg gtgacggttt gggtggttcg cattcgagac gaattgcatc
    19201 tgcgcactcc tgagcgccgg tcttggccac cacggagacc attcccccac gagccgcgac
    19261 ggagagcctg ggcactcagg tggaatcata acccgacaca gcgaaggctt gcgagactgt
    19321 ccacccgtcc agaatgagag gcgcccgcac agtcgctgac acccggtgac attgagcgct
    19381 taactgccga gtctcactgc gctatcttgc ccggcatgtc gcaagtcgag tcgtccgcag
    19441 ctgtgcctgc ttcgaatccg tcccccactg ccgtcgcccg tcgtgccgct gcggaagccg
    19501 cggtcgcact tctgaaacca ggaatgaccg ttgggcttgg gactggagac accgcggcgt
    19561 atgccataag ggcactcgct gcgcgaaagc tcacagggct gcgctgtgtg gccacatcga
    19621 ctcgatcggc ggacctggcc cgcgaactgg gtctgccgct gtttgaactg gacgacatcg
    19681 caccagcgac ggcagcaacc gaaaaacaac caatcgacat cacgattgat ggtgccgatg
    19741 aaatcgacgg cgtactccag ctcagcaaag gcggtggcgg tgcactgctg tttgaaaaac
    19801 tggtcgcaca ggcatcgcag agcatggtgg tgattgccga cgaacaaaaa gtcgtccgca
    19861 cgatcggcga cttgcggcca ctggccatcg aagtggtgcg ctttggcgcg ctgcataccc
    19921 tggcgcggat cgcccagctt ccgcaggtac agcatgcgca gcttcgccga gtcgtcggca
    19981 gtgaagcgct gtttcacacc gattctggca acttgatcgt cgatgcgcag ctacagcttc
    20041 ccccaagcac agacgccgcg cgaacgctcc atcacacgct gaagcagagc ctgggcgtgg
    20101 tcgaaaccgg cctgttctgc gatgaggccg cgctggcgtt ggtcggtcag cccgatgggt
    20161 cggtgcggcg tctttcgcgc caaagtagca agtgacctac cgacgtgtgg taaagtagcc
    20221 ggacctttac catggaatgg cttctcatcc cggcgtttgg ggtcttgatc tggctgcttc
    20281 aacgcagcgt ccagcaagaa caagcgctgt cgacgctggc caagcggctc aaatcgactg
    20341 aagagctggc gacgtaccaa gcggaacgcg cggcgaaaga agtggagcgg ctgcgcacgc
    20401 aagtgctcga gctgctcaaa gcgcacggtc agcttcagcg acggctgggt gacattcaag
    20461 aagtggtcaa tgcggcaccg gaaccgagcc atgcacagcc aatcgcgccc gctgtagtcg
    20521 tcgagcagtc gccaagcgtg caacgaacgc cccctccgct gctgccttcg accaccgaac
    20581 cgctcgtcgt cgaaccaccg gttgcggcgt cgatggcccc agaagctgtc gagaccacgc
    20641 gcacactcgc cgacgcggca ttccaggcag cagcttccgc gggtctcacc gattcggcag
    20701 cgggcgctga taccaccgat ccagcgacgg ctccgtcacg gcccagtcag ccagcgcctg
    20761 cggacacgat cgattctgaa gttgcacaag cggtgccgcc cgcgcccgct gcgatcccca
    20821 caccaccaca accgtcgaat cctgcgccgc caccggcgat tgttccacct agcgcaccga
    20881 ctttcgattg ggaacagctg ctcggagtcc gcggcgcggc atggctggga ggcatcgcac
    20941 tcgccatcgc cgggacgctg tttgcaaagt actcgattga aaatgggctg attgggccaa
    21001 cgctgcgggt catcttgctg acgctgctgg gcctggctgc gctggtcggt tccgaatttt
    21061 ttatgcgtcc gcgctttgcc gtcacagcca acgctgcgtg cggtgccggc atcgccatcc
    21121 tctacaccgc gttttattcg gcgcacggac tttacgatct tttgccgatg gcgccgacgt
    21181 tcgggctgat gctgctgacg acagtggtgg cagccctttt gtcgcttcgc tatgactcgc
    21241 tttacatcgc ggtactgggg ctcgtcgggg gctttgcgac gccgctggcg ctatcgacgg
    21301 gacaggatcg accgatcgga ctgtttgctt acgtactttt gctaaacttc ggctttttgt
    21361 gggtggcgat ccagcgacgg tggcacaagt tgctttcgct gagcctggcg gcaacactgc
    21421 ttatccaaac cggctgggca attgtccatc tgtcgccgga aaagctgccg atcttggtgg
    21481 caacatcgac ggtgttcgcc ctgctctaca tctcgatccc gctttttgta aaacagtttg
    21541 agccggaaaa acaccaagcg ctgctgtgga atgcgacgtc ggcggcgatc tggccgtttc
    21601 tgtttgccat cgtgctggcg acgcggcccg aatacgcgca gcgctgggca ctgctgtttg
    21661 ccctggtggt ctgtctcgac atcgggttgc tgattttggc cgtcgagcgc tgggctctgc
    21721 ttttgtggcc ggcgctgttt ggttcgatct tgacccaggt cagctgggcg tcgactgatg
    21781 cagctgtcag tcggctgccg gtgctggccg gggtgtcgct tctcttgatg gtgctatttg
    21841 gactcgctcc aatggtgcac cgcttccgaa agccaagtca ggagattccc gaaagcttgc
    21901 acttgaccgc ggcgggcgcg tcgctgtggc cgcttgtggc cggtctgctg cttctgggca
    21961 gcgactttgg ccagcggcac ggtcttttgt atggctggtt gcttgtggcg tatttgtggg
    22021 ctgcggtgac gggcacaagg gaaggccgag tcctgcgcat catcggggta tcgacgcttt
    22081 tgtgtctgct cggggtgcag tggacgcagc gctttgtcag cgccaatgtg ctctggccgg
    22141 cgacgctgct actgatggtt ccggtgctga ttgggaatct tttgccacgg ctggccccgc
    22201 acctctgcca aaaagacgta gatccgcaga aatcgggaac ccgtgcgacg ctacatgagc
    22261 tggcagcgac gatcctgatc attggtgtgg tgggacttct gggtgtgcag atcgccaaag
    22321 gtctggccga gccgccgtgg cttttgctgc tggtggtgac gatcgtgttt gggctgctcg
    22381 gggaacgatc gcgggtttca gcgctgccgc tggtacagcc ggtgatgacg ctgctcgtcg
    22441 cggcgctgct gtgtgggtgg cagtttggtg tgtttgattc cgacgctgat ctggtcaaag
    22501 tcaagcccgc cgagcgtgtg ctgcgggatc tggcggtgcc acttctgttt gcggcagcga
    22561 tgcagctgcg ggcggcagtt cgatcgctgc gcggatcggc agtggccccc gcactggaag
    22621 acctgggtcc gggcagccgc gaacagatga tctgggacag tgaagtgtcg gcaactgtgg
    22681 caacgctcgt cggcatgatt tcggcgatgt tggcgcttat cgactcggga ctgagccagc
    22741 gcccagggct cgcgctgctg acgctgcttg cgtacaactt gctgctgatc acgacgatgc
    22801 tgcggcagac ggtgacgctg ctattgctgg tcgggcagct gctatcggca atcgcgctga
    22861 tttcctggca acatgaccac ttccaagccg tagatttccc gacggtgatg ccactttatg
    22921 tggtgttcta tctgtggttt ctgctgttgc cgctgggtct gctgctgttt gtgccgcgac
    22981 tgcgcgctcg acggagcctg tttttcgccg cgggcttggc aggtccgatg ttcttctatc
    23041 cgctgtaccg cgcgtggctg tccggtctcg gcgatctggt gattggcgcg ctgccggtgg
    23101 tgatggcgac gctgtctttg gcaggactcg cgattgtgca agcgctgccg cgtccggggg
    23161 tcacacgcaa gctcagcgcc gatgaaatgg ctgaacggca cctggcacat cgcgcgctgt
    23221 tcgcggcagt gggcgtcggt tttatcgcgc ttgcgatccc gctccagttg caaaaacagt
    23281 ggattggagt cgcctgggcg atcgaggctg cggcggtgtg gtggatgtat cgtcgactgc
    23341 cgcatccggg gctcaagtac tttggcctgc tgctgtactg cggtgtgatt ttccgtctga
    23401 ttccgagtgc agcgtttctt cagtaccacg agcgcggctg gccggtcgtc aactggctgc
    23461 tgtacagcta cggcgtgcct tgtatctgtt tcctcgtcgg tgcggcaggt cttgccccag
    23521 tcgaagacaa ataccgtcgc cgattcgagg acacgctacc gtttgggcga ctgccgctcg
    23581 cgggaatcgc gtactacacc ggcctggtgc tgattttcgt gctgatcaac ttggaaattg
    23641 ccgatgcatt ttccgtcggt cgttacaccg agctgtggca agaacgcagc tacgcgcgcg
    23701 acctgacgcg gtctttgtcg tgggttttgt atgcgctgac cctgcttgtg accggcatgc
    23761 ggcaaagcgg acgcggacag cgcttctttt cgctcggact gatgctgctg accgtcggaa
    23821 aagtgttttt gtacgacctg gcgaaagtcg gcggaattta ccgcgcgctg tcgtttctgg
    23881 gcctggccgt gtcgctgttt gtggtgtcgc ttttgtatca gcggctggcc ttccgcaacg
    23941 cagcctcggg caaagatcaa aactccaaag ctccgtctcc ggagaagcca acatgagcgc
    24001 tttctcgcat catccaacgc tccgccgatg ggtgcagctt gcgtcgctgc ccctggttct
    24061 gtcgctgctt ggcgtgctga ttcagccgtc gcacgcgcag ggaattgcga ccgacgccgc
    24121 ccaatcgagc tttccatttc ggcgcagcgt cgaagtgcgc ggcagttcga tggacggttt
    24181 cgcagtcttt gccctgcccc ccgaacttag caacggcgat gccgaaggtg gctatcgact
    24241 gattcgcagc gacggacgcg aagtgcccta tgtcgttacc agtgacgccg gcgaatccaa
    24301 agatcgtcgc cacagtggcc agcttgtcga tacgcggcac gaaggcgatc agacgctgtg
    24361 gatcgtcgac ttgggatcga ttcggcgctt cgagagcctg gatctgaaca ttccccagat
    24421 ccagttccgt cgccgagtca ccgtcgaagc cgcagagcag caaaacgggc cgtatcacgt
    24481 cattgcgcgc gacgttggca tcttcgatct gccgtggcag aacgcgccgg gtggacggct
    24541 gcactacacc acggttcgct ttgccgaaag ccagcgcgcc cgttttctgc gcttccaaat
    24601 cagcagtcgc tggcgcggtt ttttgttccg tccgctgtcg ctacaaggtg ttgctgccgt
    24661 cgctcacctg agccgtccgg gatctgagtg gacgcttccc gcgccgcttg tgccgatggc
    24721 gacaccgccg ctgcccaagc acgtaaaacc aggcgatcat ccacatcttt cgctgtatcg
    24781 aatcgacgtg ccgccaggcc tgtcgttccg ccacattcag ctttcggcgc gcgatccggg
    24841 ctttgtccga tcggtgcggc tgctggaaat cgaagacaac gcatcgacgg tccattcttc
    24901 gacgggcaag attcaggtgt tgtcggaagg catgattttc cgaatcagcg acggacccgt
    24961 gccaacaagt ggcggcaaga aagccgacaa aggggaaaag accgacccca tcgacgacga
    25021 acccgtcacg ggcgaaaacc tgacgatccc agtcgaagac aggcccggca aaggcatcct
    25081 ggttctggaa atcacggacg gcgacagccc ggcactcacc gatctaaccg ccacggtatc
    25141 gggcatcggt tcgcggttga ttttcccagt aggtccgtcg gccagtcgcg aaagcgattc
    25201 ggcgcagaaa tacgccctgt attttggcag cgagacggca accaagcgcg actatgacct
    25261 gcaaaagctg gtcagttcgc tctctcgact ggggagtttt ttgccagccc agctcggtgg
    25321 caaagaaccg aatccacgct tccaaaaggc gcagccgctc cctgcggttc cgacggcggg
    25381 cagcccgatc gtcacgtcca gctatcggct gatgcgcacc gtcgcgatgc gagacggtgt
    25441 cgagctgtat tcgatccagc tttcgcccgt ggacatcgcg tacctaaggc cggattttgg
    25501 cgatctgcgc gtggtcgatc ccgacggaca gcaagtgcca tatgtgctga ttccaaatgc
    25561 gatcgaaacc cgcaccgagc tggagttcgc caaagaaccg gcaggcgacg gcaaaatgag
    25621 ccgatatcgc ctgtctttgc gccatggcaa ccagcttttg caggtgccga tcgacgcgct
    25681 cgaactgtcg gttggcagct cgttttaccg tcgcaccgtg cggattcgcg aggttcgttc
    25741 ggaagcgacg ccgtatgcct cgcagctgct cagtgcgacg atcttgcggc tgtcagaaga
    25801 tgacgcgggc tttcatcgct tgccgctgcc cggccagccc gtgcgggaac tgctgctcga
    25861 aattgacaac ggcgacaacc caccgctcga catcacgcag gtgtttggcg tcgtatcggt
    25921 gccgcggctg atttacaaag gcgacgcgca gaaagaatat cggctgctca tcggcaatcc
    25981 cgacgtgcaa gcgccaagct acgacttggc gagtctgcgc aacgtgctgc tcgactatcc
    26041 ggcgcggctt gtggtgctgg gcagcttgat ggccaatccg aacttccggc caccgagcaa
    26101 ttacttccaa gaaggccgga tgaacctggt tctgcttggt gtgctgctgc tatgcgtggt
    26161 ggtgctgctg gcgctgtcgc tgcggctctt gcgcaatccc catcccgaaa aacccccgcc
    26221 gatcgacacc gacgggcaga acccgccgcc aagtccgcca gcagcctagc gagctctttg
    26281 cgaatcagat ccgcagcgcc gggtccgaaa gccgcgcggg aaacatcagc ttatgcagaa
    26341 gattcagcgc cgccgccgtg gcgatcttgc ggatctgctc gcgatcgccc ggccacaaaa
    26401 tctgcctgca gatcgtttgc gcagggcccg cggctgcaat ccagaccgtt cctacgggct
    26461 tttcgggggt tcccccgctt ggaccagcca ccccagacac cgcgatgcca tacgtggccc
    26521 ccaggagctt ccgcacgcct tctgacagtt cgcgcacgca cgcttcgctg acggcaccgt
    26581 gatcgcgcag ggtcgcgtcg ctcaccccca gcacggcttg tttgacttgg tttgcatacg
    26641 ccaccacgcc accgaggtaa tacgcagacg aacccgcgat cgcggtgatc gtctgaccga
    26701 tcattccgcc ggtgcacgac tccgccgtgg ccagggtttc gccccgcgat ttgagcgctt
    26761 cgccaacgac catcgccaga tcggcatcac cggttccata cagagcgtgt cccagacggc
    26821 gacggacttc ggcatccagc acggcttgcg cctgcgcgac ttggttcccg tctgaaccgc
    26881 tgagcatcag cgtcaccagc acttcgggga acgccagccg atagtgaatc gtgacttcca
    26941 ccgtttcggc atgccgcaat tccgccgtcg atgacagcaa gtcttgcagc cggtgatcga
    27001 catgcgattc accaagcccg agcgaccgat acacgctgcg gatcaccgtt cgcggcggca
    27061 agttggtgcg aatatggggc accacttgct cgctgaacat cggcttcatt tcccgaggga
    27121 caccgggcat aaaaaagcag cgcgcaccac ctatctgaac agcaaaaccc ggcgccagcc
    27181 ccgttcgatt ggccagcacg aaagccccag ccggaatgcg cacttgccgc aggttgttcg
    27241 gcgtaatcgt gaacttccgt tcggtaaagc gctgccgcat ccgtgcttca tgatccggtt
    27301 ccgaaaccgg cgaaaccccg agcagcgacg aaaccgcatc gaccgtcagg tcatcacttg
    27361 tcggcccaag accgccgctc gcgacgacaa actggctggt cttggctgcc gttttcagac
    27421 attccacgat gtggggcaga cggtcgttgc agccgatgcg ctggcggaca tagacgccaa
    27481 gtccagtcag ctcggacccc aagaaagcgg cgttgctgtc gacgatttca ccgcgacaaa
    27541 gctcgtcgcc aatcgtgacc aaggtggctt cagcctgaac atcggatttc tcggaattca
    27601 tgataggccc cactttagca gcaaccacct ggaaccactg gccatggcgc tgcatgtgcg
    27661 gctcgcgaag cacgatttca cacggtctag aaaaagtagt ttttcgccac atgatcgggt
    27721 tccgcagatt cccgactgac agcacggtct ggttgacttt ctcgtcaccg ttgataaagt
    27781 cctactctgc gctgaagatg cacaacagcc tggcgccttt accatgaaga ttcgctacgc
    27841 agccaagacc gaccctggaa tgaagcggca gcagaacgag gactattacg acacgttcga
    27901 agaggaaaaa ctcttcatcg tggccgacgg tatgggtggc catgccaaag gcgaagtagc
    27961 ctcgaagctt gcaaccgaga cggtcaaaga gttcttcaag cgcacccgcg acgaagaagc
    28021 gacttggccc tacaagatgg accggcagct ctcgtatgta gagaaccggc tcgtctgcgg
    28081 catcaagctg gccaactgga aggttcacga atcggcgacc aaagacccga tgtgcaaggg
    28141 catgggtacg acgatggtga cctgcatgat cagcggcgac aagtcgtact ttagccacgt
    28201 cggtgactcg cgtggttatc gcatccgcga cggcgtcatc aagcagatga cgcgcgatca
    28261 cagcctcctc gaagactaca aggaggccaa gcccgacatg acggaggagg agcagaaaaa
    28321 cttccctcac aagaacgtca tcac
//
LOCUS       DAORGN010000048        28251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_641_length_28251_cov_6.406689, whole genome shotgun sequence.
ACCESSION   DAORGN010000048 DAORGN010000000
VERSION     DAORGN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 28251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28251
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_28251_cov_6.406689"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PKL17_07595"
     CDS             <1..774
                     /locus_tag="PKL17_07595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44629.1"
                     /translation="LPVCDVLIEAHAATLVHRDIKPENVFLHKTRSGEVIKILDFGIA
                     KMLGEGQDLDRKLMTGGGGIIGTPTYMAPERLRQGSYDGRSDVYSVGVMMYLMLSGRL
                     PFTSDKESYVEIVLGHLNQPVPPLVGGIDPIPGFFTQVVMRTLEKDPSLRPTARELLN
                     DLVRVARYGLDAGGLLGDVAVPRSGQPSQSGAPSEEIDAERTGELASSNGEAVSSPSA
                     KTLPPATAAVLSRTIEEDPPAAIAHERTQDFSRPGTNAS"
     gene            complement(796..2172)
                     /locus_tag="PKL17_07600"
     CDS             complement(796..2172)
                     /locus_tag="PKL17_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44630.1"
                     /translation="MPPFVRRLSLGAAFLGLAGGILLSPPKSRAEGEVTQTQAAVAQP
                     LGSESKAGAQALLGAVDEITRKVVTIRGLPTKTTFARGVLSRQEITQRLKERIHNDYS
                     PGEVQGEALVLKRMGLIPVDADYEKLLFDLLSEQVAGFYDPYRKTLYVADWLPIDMQK
                     PALAHEIQHALQDQHFDLKKLAKPLKNEGDRQLAQAALVEGDGTAVMMEYAARNMGVE
                     SAQLPQVLARLGRQMMQLAISTTPSLQNAPAVLRESLMFPYAAGLEFVAAVRGDKPWA
                     RIDQAFREPPISTEQVMHPEKYVAAEKPWKVTATLLASLPQYKEQRRDVFGELMWKVW
                     FGRAMSDRDAEQAAAGWGGDLLISYAQPGETLPLVALFSGWDTDKDADEAEQAAQKLV
                     LSLTGKTGDKLPAPGKVAMFERSVGDRAFVVARKDKRLLILCGAPTASTSAVVAEVWH
                     SWSATPAP"
     gene            2270..3475
                     /locus_tag="PKL17_07605"
     CDS             2270..3475
                     /locus_tag="PKL17_07605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK44631.1"
                     /translation="MPSQTQPSLRRETAALRVALVSEYYYPDIGGMPEHVHQLGRALA
                     RCGHQVTVITTEFPGRVDGPTDVPYEVVRLGRACPPLYANGSLSLSAVGWGLRRRLQA
                     LFRQRRFDVIHVHAPIFPTLALLAISCAPPESALIGTLHTHFVDSSLLRFFRPALQRY
                     LDALDGLIAVSDTAVDCMRRIGFRLDAEIIPNGVDFDSWQSGTSLPSLRGNSSLHLLV
                     QARLEPRNRIETVIAALSALSHDGQQRRLSVLGEGPRRQELVSLSHRIDAQFPGAVVA
                     ARPDYAASADVYSFTADIASHPMSLIEGMAAGLPVIAHPISGVRELVSDGKEGLLVPV
                     GDAAAYAQAIRTLDASPSLRRQLGDAARAKVAWLDWRRLVKPIESQYLRAISSRRASG
                     RAGSASVWP"
     gene            complement(3438..4562)
                     /locus_tag="PKL17_07610"
     CDS             complement(3438..4562)
                     /locus_tag="PKL17_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK44632.1"
                     /translation="MRRIFLAMSLLISLSACEPSGADKRPGRPPPLTLVSRPVQRDVP
                     VEIRAPVDLRPREQADILSKQVGYLSAVLVDRGDVVKKGQLLALVRPSELPDQLQAAR
                     GQLQQAEAALEQAKQNRTRIGALAPAGVVSTQELQQSSTALSQAEAAEAAARAQLQAV
                     AVRLGETKIESPIDGVIMQRRFDAGALVGPQNGAIVTVGRVDPLRVFVAVREQEAAKV
                     GIGQKVLLEVDAQPGEVFSGAVVRLSPGFDPLSRTLEAEVHLPNSDGKLRPGMYGRAR
                     IQVGLHKNTLTVPESAVQLSGDQRFVFVIVDGKAQRRAVTLGVDGGTFLEIVSGLKTD
                     DSIVVAGIDALADGSPVRTQSGTTPWQPTTAKPTPSPPAH"
     gene            4699..5166
                     /locus_tag="PKL17_07615"
     CDS             4699..5166
                     /locus_tag="PKL17_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNK44633.1"
                     /translation="MQQIRFDDVAALQARVGEPYSPWGPEVTITQDMINRFADVTIDH
                     QWIHIDVERAQKESPLKCTIAHGFFVLSLLPHLRQRVDMQVVGYRNVLNYGADKLRFL
                     SPVPAGSTVHARCRVVSVEAKPKGTLLSEEIEIAVVGQDRPALTYTMLMLFQP"
     gene            complement(5167..6153)
                     /locus_tag="PKL17_07620"
     CDS             complement(5167..6153)
                     /locus_tag="PKL17_07620"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNK44634.1"
                     /translation="MVHPSPIFAVDDESLERAANLLRSGEVCAFPTETVYGLGADAEN
                     EAAVLRIYALKGRPRQNPLIVHVESLAAARTLCSDWSTLAERLGQRFWPGPLSLVLPR
                     APHIPALVSAGLPSVAIRVPAHPVALALLTKTGRPLAAPSANRSESVSPTTAQHVLRS
                     LPNVPMILDGGPCRLGIESTVVSLTQNPPRLLRPGALPLRLLLEEIPDLALPGWEKET
                     PTGALPSPGLLSRHYAPSAPLFLIRDDDAPSVAQLPRPIGLITHTGLPTVASQCQDIE
                     LLPSTPEAYATDLYAALHRLDDKQVKSIVVLRPPQDNDWRAVIDRLDRAAAS"
     gene            complement(6173..8614)
                     /locus_tag="PKL17_07625"
     CDS             complement(6173..8614)
                     /locus_tag="PKL17_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNK44635.1"
                     /translation="MRTLRISRLCAFASLAVCLPVPVALVVPTSQAHAASGSTTTISP
                     NVVSRAMDGTPRLLLGSLTAASPGTAPLTVAERYLVQLRQSSPTWVPQELSEPELIPV
                     GTGHVVRYRQQHHGLPVWGSELVLRISTDGQVERLFRDVVPTDALAKIDPVPKISPQD
                     AQQAVRALGPGQIGEPVLMIDNQNRRLAYLVSRMELSALEHGDYLVDAQSGQVIRRIE
                     RLNFLNQFGVYRYNPGTTSNIDQFGIPSGDAGPFAPTGTGARGMTSTLIKGLNCVDDF
                     TTRLPGGGTTKLHLCTPGQSLSNTSGDYNQFLPLIGANDGHCPTLNDANKNSFGEAHM
                     YWHTANIYTRFRTLFGALGIADFKLRISTGATPKPFPLLTNLCMPDLTNAAKATNPNI
                     ALSPFENAFYSPGDSGGGYSYFILGVPGDMIAFGMGAKANYSMDADVIYHEFTHAVIS
                     TRNRLVGSVGIDNYGLNDDPGAMNEGFADFFSSATTGNSPVGEYAKQNFGLATVGLRD
                     VDNNLHCSNDRVGEVHEDANPWSGALWRARKAVTGDPLDTSATAVQKRQAFDQAVLAA
                     IEGSVMGTTMTEMGTLVVNEVGKLAATLGADASQKTTDAMTTQGVLSLCDRIIRVQTP
                     HKMLCLDSDAKKVWPGHAQWKIDMPASADTVTFDFTTLAAGAVCNNIDPTATSQAPKL
                     QIAQRLDGGPITWNMTGAGTYDKLIDVKQGATNNSWTVTVPLAKSKTFHLMLVNSGGP
                     RVAQNVTMTMSCAAPDGCPVPADADGGVTQVPLTTPTGCGCHVGEASRATSALSLLSV
                     GGLLAALVSRRRRRA"
     gene            8808..10898
                     /gene="fusA"
                     /locus_tag="PKL17_07630"
     CDS             8808..10898
                     /gene="fusA"
                     /locus_tag="PKL17_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNK44636.1"
                     /translation="MISDLSRVRNIGISAHIDSGKTTLTERILFYTKRIHAIHEVKGK
                     DGVGAKMDSMDLERERGITIQSAATYCSWNNHHVNIIDTPGHVDFTIEVERALRVLDG
                     AILVLCSVAGVQSQSLTVDRQMRRYRVPRIAFVNKCDRTGANPLRVKNQLREKLGHNP
                     VLLQLPIGLEDKFEGVVDLVTMEAITFTGDNGQEIHRNPIPADMKSQADKAREEMLDT
                     VSMFSDALTEAILEDRVTPELIREAIRRGTLDMKLTPVMMGSAYKNKAVQLLLDAVTY
                     YLPCPSDVVNEAIDIDSPDNKKVTLQSDSSLATVALAFKLEDGRYGQLTYMRVYQGAL
                     RKDDVIHNTRTGKKVKVARLVRMHSDDKEDISDAGPGDIIAMFGVDCNSGDTFTSGVQ
                     LAMTSMYVPDPVIKLAIKPADQKAQMNMSKAIQRFTKEDPTLRCEVDPESGETLLAGM
                     GELHLDVYIERMKREYNAVVQVSPPQVAYRETVTQRADYNYTHKKQTGGSGQYGKVMG
                     YIEPCAEKDFEFVDEVVGGTVPREFIPSVEKGFRSMVAKGQLIGFPVVNVRVVLADGG
                     SHAVDSSDIAFQEAARGAWREVYQKAKPQILEPIMKVALEGPSEHQGNMLSTLMSRRG
                     MIIGTTEADGMSTIEAEVPLAEMFGYATTLRSATQGKAEFSMEFSRYAPVPGGLAEEL
                     IAKVAKAKAAEKKK"
     gene            10913..12250
                     /locus_tag="PKL17_07635"
     CDS             10913..12250
                     /locus_tag="PKL17_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44637.1"
                     /translation="MYRKEVNERSPLRILEKSSHGGLGRGNLGVAMARAGVGKTAFLV
                     HVALDDLMRDRKVLHVSLDSPVDHVKSWYDAVFQDLVKTADLADHAVTQELINKNRII
                     QAYSLHGHGGANEFSIAKLKHSIELLSQHAQFVPDVIMIDAFDWSRATLEEAAELRAL
                     AKQFNVELWMTALTHRHQTGPEVPDRVPPPCDRFASLISLVVFLQPVDKNLSVRLLKD
                     HDNEVVCDTHLVLHPDTMRLVDDRAPSSPASDLPPSAYTLLSGGAIGAESEFGACAER
                     YGLKEENYSFEGHPVARTRGLRLLTNEELRDGDVSLAYVSATMHRPYHQNPTLRKVLQ
                     SIWHQVNSSQEVFIIGVIQRDDTVKGGTGWAAELARHQGKPLHVYDQERRSWFTWNHT
                     TRQWQASKPVITHTRFTGTGTRSLSDDGRAAIHELFAQSFGAARTGRSGPIKA"
     gene            12253..13485
                     /locus_tag="PKL17_07640"
     CDS             12253..13485
                     /locus_tag="PKL17_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNK44638.1"
                     /translation="MRAGQPRLCKLLRAVLPIVVVLLAFAFPQTAFSHQSSVVFLRLV
                     PERHTLHVTLRIANSDLFEALGLPADHVVLPEDVHRQREQLTAYLLGRVSVANLGVGC
                     PGRREQSAFEPRQGGFFAVETLAFECPRALEKSTLRYDLFFDLDPRHQGMALVELPGA
                     PREYIFRENSRLLTLDQPRTVLDYALDYLRLGIEHIFTGYDHLAFVIGLLLLVGSLWR
                     KTSPRHALGYILRVVTGFTVAHSLTLILSALRFVSLPSRWVESLIALSIGYVAIENIV
                     GVKVQRRLWLAFGFGLVHGLGFASVLRELGLPSRGLVVSLLCFNLGVEVGQLTVVLLL
                     LPLLALMSRSKWYPSVMIRGASWILLALSLIWFVERAFDMQWERGRAAIAWKRVSGDD
                     PTAFARHEIRLTTTANRS"
     gene            13537..13854
                     /locus_tag="PKL17_07645"
     CDS             13537..13854
                     /locus_tag="PKL17_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNK44639.1"
                     /translation="MATMITEDCINCGACEPECPNEAISEGEDLYVIDPNLCTECVGF
                     HGSEACQAVCPVECCIPDPNNRESEETLLNRAKKLHTGEKFPELHELNAKNSLFRNSA
                     RKH"
     gene            13988..17608
                     /gene="nifJ"
                     /locus_tag="PKL17_07650"
     CDS             13988..17608
                     /gene="nifJ"
                     /locus_tag="PKL17_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNK44640.1"
                     /translation="MPAVTIDANEAVANVAHRLSEVIAIYPITPASAMGELADEWSTH
                     GRKNLWGEVPRVVEMQSEAGAIGAVHGALQAGSLATTFTASQGLLLMIPSMFKIAGEL
                     LPMVLHVAARTVATHALSIFGDHSDVMACRSTGFALLCAGSVQEAQDFAALAHAATLQ
                     TRVPILHFFDGFRTSHEIAKIEPISDDTLRSLIPETGIAAVRERAMTPDRPVLRGTAQ
                     NPDTFFQAREACNPFYEAFPAALKQHMARFADLTGRRYELFEYVGHPEADRVIVTMGS
                     SSECVHETVEYLQAQGQRVGLIKIRLLRPFSLVDFARVLPKTVRQIAVLDRTKEPGAV
                     GDPLYLDVVTALSEAVSDGLYPHLPKVIAGRYGLSSKEFTPAMVVAVFSELAQASPRR
                     HFTIGINDDVSKRSLDFDDSLGIEPADVVRAVFFGLGSDGTVGSNKNTIKIIGEETDH
                     FAQGYFVYDSRKAGAVTVSHLRFGPRPIRSTYKIRRAGFVACHQPQLLDRFEMAELAD
                     EGSTFLLNAPWPADRAFGFLPREVQQLLIARKMRFFVIDGQRVAEEAGLGRMISTVMQ
                     TCFFALSGVLQKEVALDKIRQAAQKSYGKRGSEVVRRNLAAIDRTLDNLFEVAVPELP
                     STQRSRPDIVPKTAPDFVQRVTALMLAGHGDYLPVSAFPVDGTWPLGTAIYDRRSLAK
                     EMPTWDSRVCIQCNKCVLICPHAAIRSTVCDEKQLGDAPAGFVVVDYKERDALPGQKY
                     RLQVAPDDCTGCGLCVQFCPAKDKTNPRHKAIDMSPAEPLKPVERETFAFFQKLPAPD
                     RKTVHLDVKGSQFLPPLFEFSGACAGCGETPYLKLLTQLFGDRLMVANATGCSSIYGG
                     NLPTTPWSKNADGRGPAWANSLFEDNAEFGLGFRLSIDQHTAYAQSLLRGLSGELSDE
                     LVTAILTAPQDSEASLAAQRERVVELKNRLQRIARPESALLQALADYLVKKSVWIVGG
                     DGWAYDIGFGGLDHVIASGTDVNLLVLDTEVYSNTGGQQSKSTPIGAAAKFAAAGKEL
                     PKKDLGMHAMMYGHVYVARVAMGAKDAHTVKAFLEAESYPGPSLIIAYSHCIAHGYDL
                     QMGAEQQRLAVDSGVWPLYRFDPRRLDVGEPPLKLDATPGKAKVQQYMANEGRFRMVE
                     LADPARYKQLLKAAERDVAMRQAMYQHLQGLQVPVPGANKPAQSDKPATPSDKPTGAG
                     SH"
     gene            17611..18609
                     /locus_tag="PKL17_07655"
     CDS             17611..18609
                     /locus_tag="PKL17_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNK44641.1"
                     /translation="MNLATSYLGLKLAHPILPGASPLADDLDSVKRLEDAGAAAIVMR
                     SLFEEQVAQESLGAYQHLDGHGDLYAEAQSYFPSTNVFALRADAYLEQLRRIRQSVAV
                     PVIASLNGTTPGGWLDYAKQIEQAGAHALELNLYSLPSSPEHSAHDVERGQLQIIAEV
                     ARSVRIPIAVKLSPFYTSLPHFVAEVARTGARGVVLFNRFYQPDIDIETLDNVPQLHL
                     SDSSELLLRLRWLAIVSPRTPLSLACSGGVHSATDAVKALMAGAHAVQVVSALLQHGP
                     GTLRKIVDGVSRWLTEHDYESMDQLIGSMNHARSPNPSAYERANYIRLLSSFHPQD"
     gene            18928..19827
                     /locus_tag="PKL17_07660"
     CDS             18928..19827
                     /locus_tag="PKL17_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNK44642.1"
                     /translation="MSEQTAQSPARPFVKWAGGKRQLLPDLLHRVPSSFRHYHEPFVG
                     GGALFFALTEQNRLDGKGVRLTDINRELINTYQMVRDRVETLIALLSTFKNDESFYYT
                     IRALEPSQLDEVQRAARFIYLNKTCFNGLFRENRRGQFNVPFGRYKQAQFCAPNDLRA
                     ASRALRGVCLEVAGFDEIAMSCQPGDFVYCDPPYVPVSRTASFTSYNGGGFGDEDQQR
                     LADVVRVLGERGVHVLLSNSAVPLTYALYRELHIEEVLARRAINSRGDRRGHVPELLV
                     AAGPLASELLARRRPGAPASLAK"
     gene            19889..20545
                     /locus_tag="PKL17_07665"
     CDS             19889..20545
                     /locus_tag="PKL17_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44643.1"
                     /translation="MTHRVPVRLLCGPLCGLLGLLSSCTLSESYLVPSKAIELVHSQP
                     PDSRQRAQLPALRESDRSAALIRYRALEWSGETLNRVAATPMTMARVRVAKVNPLMIA
                     GGTILALSVPHLALGMAAGLDLPPGETKPADPIGMGIGLGLGGLHVFVGGLLMALGER
                     RPNIEPTEPALVKAYLRGEIPTATAAQEAVTETGEAAPPPDPPRRSTYNVPLSPDDGK
                     "
     gene            20561..21898
                     /gene="purB"
                     /locus_tag="PKL17_07670"
     CDS             20561..21898
                     /gene="purB"
                     /locus_tag="PKL17_07670"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNK44644.1"
                     /translation="MISRYTRPELAALWSESRRLALFLEVELAATAALEEAQSSPIPT
                     GTAMRLRQLSAQMGPLDPSRVSEIERRTQHEVLAFLEHVEERLGPEARWLHFGLTSSD
                     VLDTVLALQLREATSIIIRGIDEQLLPALRRQATAHQQTPMIGRTHGIFAEPIAAGLV
                     FLGSYAEIERAKARIQRAQRAIAVGKLSGAVGVYGSGCVTPDVEEKALSSLGLVAETV
                     ATQVVARDRHAELLWSLSMLGAAVERLAVNLRHLQRSEVSEVEEAFAPGQKGSSAMPH
                     KKNPISAENLCGLARLLRANAHAAVENIALWHERDISHSSVERVIMPDSTTLCDYMVQ
                     RAAKLVFGLSVRTGQMDRNLAQSGDLYASEKVMLQLVRSGLSRQSAYELVQRHALACV
                     AERGSADAPSFQARLAADPQIADRLPTSVLAACFEIATQLQHVPQIFARTLAD"
     gene            22065..23273
                     /gene="clpX"
                     /locus_tag="PKL17_07675"
     CDS             22065..23273
                     /gene="clpX"
                     /locus_tag="PKL17_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNK44645.1"
                     /translation="MDDFHCSFCGKRRREVRKLISGPRVFICDECVSLCNDILSKEDA
                     QERPRYPKPVEIKDELDKYVVGQGRAKKTLAVSVYNHYKRLMSRGRAGDVDVHKGNVL
                     IIGPTGCGKTLLAQTLAKKLDVPFAIADATTLTEAGYVGEDVESVIKALYRNAGGDIE
                     KAQRGIVCIDEIDKISRKGGGPSVTRDVSGEGVQQALLKLLEGKHATITPDGARNRPQ
                     QELIQVDTTDILFICCGAFNGLEDTIRRRVSERGMGFGARISARNDEDKDALRAQVRT
                     EDLIKYGMIPEFLGRLPIIVSCDSLDENALAEVLWKPKNALTKQYSRLFEMEGVKLRF
                     TDDALMAVAKEAYRRKSGARGLRAIMEEVMLEVMYEIPSMTGVRECVINADVITSRQQ
                     PQLIREKKAS"
     gene            complement(23347..24009)
                     /gene="rsmG"
                     /locus_tag="PKL17_07680"
     CDS             complement(23347..24009)
                     /gene="rsmG"
                     /locus_tag="PKL17_07680"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNK44646.1"
                     /translation="MQDSAVSSIEKLSPLERLLVSAAPRQGVCLSPEDARRLIRYAEL
                     LEEWGRKINLTAVVGREQIVRRHFLDSLALVGRLPESSKLPAQTLVDVGSGAGFPGVL
                     CALCRPDLQVTLSERIGKKAAFLLTLRRELGLKFDVFADDASRLPAGFGIVVSRAALP
                     LPKWLPFATDLAAPQGFVFAMTSPGEPIPALPAQLRPFVDEAYDVGEGPHRILGYRRD
                     DQ"
     gene            complement(23990..24622)
                     /locus_tag="PKL17_07685"
     CDS             complement(23990..24622)
                     /locus_tag="PKL17_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44647.1"
                     /translation="MTTRHTYPRRGKRLALSLCLVAGSVLGIYCTTTTPTPGQDPAIL
                     GRCSFPRACYLVHKSGPLQGQCDSCATGASCRLIFTADETGATSGTWKLGSAAPGPLD
                     SAAICGNYTSPSADLVALCLSPEMVCVARGPECSASSYCVAEGQDCASGVMSFPQHRP
                     GTDKTACPTEKSRCCAPVTDGGSSDGGSSDGSVSDGGVTDLSSTDAGLRG"
     gene            complement(24619..25365)
                     /locus_tag="PKL17_07690"
     CDS             complement(24619..25365)
                     /locus_tag="PKL17_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNK44648.1"
                     /translation="MLKPIGKQRVAEEIVQQLRSLILRGVYGIGDKLPPERKLAEDLG
                     VNRASLREAIKSLEQMGLVKTRQGDGTRVLPFMETAGVELVSHLVTGTADGIPNADVL
                     FDVLEFRRIFARDVAELAAERATADDLTRLEEIAKKADADVSPDEMLKLDFEFYVELT
                     RTARNRVFQLLINTIRMAVSAHAAFFAQVLPPATVQRKCHRDVIAGLKAKDTVRTAKA
                     VSDYMMQAQQLLSALLLSLGNSTDKPSGTK"
     gene            complement(25490..27838)
                     /locus_tag="PKL17_07695"
     CDS             complement(25490..27838)
                     /locus_tag="PKL17_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44649.1"
                     /translation="MFRKLKGRSGLNTLLASSMIALFAAMTGCSGSEVGNGAGLCDPA
                     CADNEECIDSICVSKTRCTVDSDCPGDSYCLNNRCAPYSADHPNNPTCERLVPAGLLS
                     PQIFCEWQKPLATDPYPNHKQVLSTPLVMDFNFAGRGSEFVTTRPSILVNTYSGTDGS
                     CGLYSGNYGILRVLDGRSCNQQYVIATPINGATTPAIGDLNGDSSADIVAYSATGGLV
                     AFGFDRAQNKFVTLWTSKTGAGADSNPFAGLCEWTGPAIHDLDDDGRPEIIAEGFVYS
                     ASGVLIDSSVNLRTTGQDTGQFSVVSDIDKDGIPNLVSPTAIYSWDKANSKWKKDRDL
                     PLPPGLSQARAYVAVADFGVPNGTTIDRNISDGIAEIAIIMSGRAWIVSKDGAIVFGP
                     LSLPGSTGGGPPTIGDFDNDGRAEFAAAGSSSYTVFDPDCVAGAEAKFCPSGRTDRLL
                     WSNLSQDVSSNITGSSLFDFEGDGTAEAVYADECFARIYSGKTGEILFSQAHSSCTWN
                     EYPVVADVVGNFRSKLIVPSNENCGVACPAVDPIFKGLRCTASTDCPNGIGCDQGYCR
                     CTADTQCNTASVGGGFVCKPPIAGVPGTGNTCQAAFTGKRTGIRVFGDVADRWVASRP
                     LWNQHTYSITNTDDKGGIPRTSQWQRNWDVPGNNNFRMNVQGKLSSSAAGDVTAPNNK
                     GTAVCQGSTVQLSTSICNRGTAPVVDGTPISFYENGILLCTALTAEALAPGLCSTVSC
                     SAPLSQSGSHTVQIIADDNGMGTGQTSECNETNNTGTMSFAC"
BASE COUNT         5394 a         8450 c         8804 g         5603 t
ORIGIN      
        1 ctgccggtct gcgacgtgct gatcgaagca cacgcagcca cgctggttca tcgcgatatc
       61 aagcctgaga acgtgttttt gcacaaaact cgctcggggg aagtgatcaa gattctcgac
      121 tttggcatcg caaagatgct tggtgaaggc caggatctgg atcgcaagct gatgaccggc
      181 ggcggcggca tcatcggcac gcccacctac atggctccag agcgcttgcg gcagggttcc
      241 tatgatggcc gcagcgacgt ctacagcgtc ggcgtcatga tgtatctgat gctgtcggga
      301 cggctgccgt ttaccagcga caaagaaagc tacgtcgaga tcgtgctcgg gcatctgaat
      361 cagccggtgc caccgctcgt cggtggaatc gatccgatac cgggcttttt tacccaagtg
      421 gtgatgcgca cgctcgaaaa agatccgtcg ctgcgtccga ccgcgcgtga gctattgaat
      481 gatctggtcc gggtggcgcg ctatgggctc gacgcggggg ggcttttggg cgatgtggca
      541 gtgccgcgca gtggccaacc gtcgcagagc ggcgccccca gcgaagagat cgatgccgaa
      601 cggacgggcg agcttgcatc cagcaacggc gaagcagtct cgagcccatc ggcgaagact
      661 ttgccaccgg ccaccgccgc tgtgctcagt cggaccatcg aggaagatcc accggcagcg
      721 attgcccacg agcgaaccca ggacttcagt cggcccggca cgaacgcaag ctaacgtcga
      781 tcgggccctt ccagactagg gcgcaggggt tgccgaccac gaatgccaga cctcggcgac
      841 gacggcactt gtgctggcgg tgggcgctcc gcataggatc aaaagtcgct tgtccttgcg
      901 ggcgacgaca aatgcgcgat cgccgacgct gcgttcaaac atcgccacct tgccgggggc
      961 aggcagcttg tcgccggtct tgccagtcag actcagcacc agcttttgtg ccgcttgttc
     1021 ggcctcgtcg gcgtctttgt cggtatccca gcctgaaaac agcgcaacca gcggcagggt
     1081 ttcgcctggt tgggcatacg aaatgagcag atcgccgccc catcccgctg ctgcttgctc
     1141 ggcatcccga tcagacatcg cgcggccaaa ccagactttc cacatcagtt cgccaaacac
     1201 atcgcgacgc tgctctttgt actgcggcag gctagcgagc aatgtcgcgg tcactttcca
     1261 tggtttttcc gccgcgacgt acttttccgg gtgcatcact tgttccgtcg aaatcggcgg
     1321 ttcacggaat gcctgatcga tgcgtgccca cggcttgtcg ccgcgcaccg ctgcgacgaa
     1381 ctccagaccc gcggcatacg gaaacatcag gctttcgcgc agcaccgcgg gcgcattttg
     1441 caaagacggc gtcgttgaaa tcgcaagctg catcatctgc cgtcccagcc tggccaagac
     1501 ttggggaagc tgcgccgatt caacgcccat gttgcgcgcc gcgtattcca tcatcaccgc
     1561 tgtgccgtcc ccctcgacca gcgccgcctg tgcgagctgc cgatcgcctt cgttcttgag
     1621 cggcttggcc agctttttca ggtcaaagtg ctggtcttgc agcgcatgct gaatttcgtg
     1681 cgccagcgcc ggtttttgca tgtcgatcgg cagccagtcc gcgacgtaca gcgtcttgcg
     1741 atacggatcg taaaaaccgg ccacctgttc ggacagaaga tcaaaaagca gcttttcgta
     1801 gtccgcgtcg acgggaatca gccccattcg tttgagcacg agcgcttcac cctgcacttc
     1861 gcctggcgaa tagtcgttgt ggatgcgctc tttgagtcgc tgggtgattt cctgacggct
     1921 gagcacgccg cgcgcgaacg tggttttcgt cggcaagccg cgaatcgtga ccactttgcg
     1981 ggtgatttca tcgacagccc cgagcagcgc ttgcgctcca gctttgctct cgctacccaa
     2041 cggctgggcg acggcggctt gggtctgcgt gacttctccc tcggcgcgac tcttcggcgg
     2101 cgaaagcaaa atcccgccgg caagccccaa aaaagcagcc ccaagcgaga gtcggcggac
     2161 aaaaggtggc aaagccgcgg cgggctgaga ccgacgatga acggcgaaac gaaaccacat
     2221 tgttgagagc ttaaccaaac ttcgacgacc ttgctatgtt gctgccgtca tgccaagtca
     2281 aacccagccg agcttgcgac gggaaaccgc agcgctgcgg gtggctctcg tcagcgagta
     2341 ttactatccc gacatcggcg gcatgcccga acacgtccat cagctcggac gggcgctcgc
     2401 gcggtgtggt catcaggtga cggtgatcac gacggaattt cccggtcgcg tcgatggacc
     2461 gacggacgtg ccctatgaag tggtgcggct tgggcgagcc tgtccgccgc tgtatgcaaa
     2521 cggttcgctg tcgctgtcgg cagtgggatg ggggcttcgg cgacggctgc aggcgctgtt
     2581 tcggcagcgc cgcttcgatg tgattcatgt ccacgcgcca atttttccga cgctggccct
     2641 gcttgcgatt tcgtgtgcac caccggaatc ggcgctgatc ggaacgctgc acacgcactt
     2701 tgtcgattcg tcgcttttgc ggttctttcg gcctgcactg cagcgctacc tcgacgcgct
     2761 cgacgggctg attgcggtga gcgacacggc ggtcgactgc atgcggcgga tcggttttcg
     2821 gctcgacgcc gagatcatcc caaacggtgt cgatttcgac agttggcagt cggggacatc
     2881 actgccgtcg ctgcgcggca actcgtcgct gcatctgttg gtacaggcca ggctggaacc
     2941 gcgcaatcgc atcgaaacgg tcattgcggc gctttcggcg ttgtcccacg atggtcagca
     3001 gcggcgactg tcggtgctcg gcgaaggtcc gcgtcgccaa gaacttgtgt cgctgtccca
     3061 ccgaatcgac gcgcagtttc cgggggccgt ggtggcggct cggcccgact atgcagcgtc
     3121 ggcggatgtt tattcgttta ccgccgacat tgcttcgcat ccgatgtcgc tgattgaagg
     3181 aatggcagca ggtctgccgg tgatcgcgca tccgatttcg ggtgtgcgtg agcttgtcag
     3241 cgatggaaaa gagggacttt tggtgccggt cggcgatgcc gcggcctacg cgcaggcgat
     3301 tcgcacgctg gatgcgtcac catcgctgcg tcgacagctt ggcgatgcgg cacgggccaa
     3361 ggtggcttgg ctcgattggc gacggcttgt gaagccgatt gaatcgcagt atctgcgggc
     3421 gatttcgtcg cggcgcgcta gtgggcgggc gggctcggcg tcggtttggc cgtagtcggt
     3481 tgccatgggg tggtgccgct ttgggttcgc acgggcgagc catcggccag cgcgtcaatt
     3541 cccgcgacaa caatcgaatc gtcggttttc agtccactga cgatttccag gaatgtgcca
     3601 ccgtcgacac ccaaagtgac cgctcgcctt tgtgctttgc cgtcgacaat cacaaacaca
     3661 aagcgctgat cgcccgatag ctgcaccgcg gactctggca ccgtcagggt gtttttgtgc
     3721 agtcccacct gaatccgcgc ccgaccgtac attccgggcc gcagcttgcc gtcggaattg
     3781 ggcaggtgga cttcggcttc cagggtgcgc gacagcggat cgaaaccagg cgacagccgc
     3841 accacggcgc cggaaaacac ctcccccggc tgggcatcga cctcgagcag cactttttga
     3901 ccgattccaa ctttggccgc ttcttgctcg cgcacagcga caaagacccg cagcggatcg
     3961 acccgtccga cggtgacaat ggcgccattt tgtggaccga cgagcgcgcc tgcatcgaat
     4021 cggcgctgca tgatcacgcc atcaatgggc gattcgattt tggtttcacc gagtcgaact
     4081 gcgacggctt gaagctgggc tcgggcggcg gcttcggcgg cttcggcttg cgacagcgcg
     4141 gtgctgcttt gctgaagctc ttgcgtcgac accacacccg ccggggccag cgcgccaatc
     4201 cgcgtgcgat tttgtttggc ctgttccagc gccgcttcgg cttgttgaag ctgtccgcgc
     4261 gccgcttgga gctgatcggg cagctcactc ggacgcacca gcgcaagaag ctggcctttt
     4321 ttcaccacgt cgccgcgatc aaccagcacc gcgctcagat agccgacttg cttggacaga
     4381 atgtcggctt gttcgcgtgg gcgaagatcg accggcgcac gaatttcgac cggtacgtcg
     4441 cgctgcactg gccttgaaac cagcgtaagc ggcggcggtc gaccggggcg cttgtccgcc
     4501 ccacttggct cgcaggcgga cagactgata agcaggctca tcgcgaggaa aatgcggcgc
     4561 acgaacctgg attagcacgg cttgccgaag ccggcatgca aaagcgtggc gtgacagtcc
     4621 ggctgcgtgc tttcggtgat ggctgggcat gcgctatctt gtcgcccgtt ttcacaacca
     4681 gcgagggaat ccaaagtcat gcagcagatc cgtttcgatg atgtcgccgc cctgcaagct
     4741 cgcgtcggtg aaccgtatag cccgtggggg ccggaagtca ccatcacgca agatatgatc
     4801 aaccgatttg ccgacgtgac gatcgatcac cagtggattc acatcgacgt cgaacgtgcg
     4861 caaaaagaaa gcccgctaaa gtgcaccatc gcgcatggct ttttcgtgct gagcctcctg
     4921 ccgcatctgc gtcagcgcgt cgacatgcag gtggtcggtt atcgcaacgt gctaaattac
     4981 ggcgccgaca agctacgttt tctaagccca gttcccgccg gtagcacggt ccatgcccgc
     5041 tgccgcgtcg tgtccgttga agccaagccc aaaggcacgc tgctcagcga agagatcgaa
     5101 attgccgtcg tcggtcagga tcgaccggcg ctgacctaca cgatgctgat gctgtttcag
     5161 ccgtagctac gaggcggcgg cgcgatcgag tcgatcgatc accgcgcgcc agtcgttgtc
     5221 ttggggcggg cgcagaacga cgatgctctt cacctgtttg tcgtcgagcc gatgcagcgc
     5281 cgcatacaga tcggtcgcgt aagcctcagg cgtcgacggc aacagttcga tgtcctggca
     5341 ctgcgaagcg accgtcggca gcccagtgtg cgtgataagc ccgattggcc gcggcagctg
     5401 ggccacggac ggcgcatcat cgtcgcggat caaaaacagc ggggcggacg gcgcgtagtg
     5461 tcgactgagc agtccaggcg atggcagcgc tcccgtcggg gtttcttttt cccagccggg
     5521 tagcgcaagg tcggggattt cttccagcag cagtcgcagc ggcagcgccc ccggacgcag
     5581 cagccgcggc ggattctgcg tcagagacac caccgtcgat tcaatcccca aacggcacgg
     5641 tcccccgtcg agaatcatcg gtacattcgg caagctgcgc aggacgtgct gggctgtcgt
     5701 cggggacacc gattcgctgc gattggcact tggggcggca agcgggcgtc cggtttttgt
     5761 aagcagcgcc agcgccacag gatgggctgg cacgcgaatc gccaccgatg gaagtccggc
     5821 gctcaccagc gcggggatgt ggggcgcgcg cggcagcacc agcgacagcg ggcccggcca
     5881 aaaacgctgc cccagccgct cggcgagcgt cgaccagtcg ctgcagagcg ttcgggccgc
     5941 cgccaggctt tcgacgtgga cgatcagcgg attttggcgc gggcgacctt tgagcgcata
     6001 aattcgcaac accgccgctt cattttccgc atcggccccc aggccataga cggtttccgt
     6061 cggaaatgcg cagacttcgc cgctgcgcag caggtttgcc gcgcgttcca gcgattcgtc
     6121 gtcgacagca aagatcgggg atggatggac catggaccaa gaagtgtcgc gattaggcac
     6181 gtcgacgacg gcgagaaacc agagctgcga gcagaccacc gacgctcaaa agcgaaaggg
     6241 ccgacgtggc gcgcgatgct tcaccaacgt ggcagccgca gccggttggc gtcgtcagcg
     6301 gaacttgcgt cacgccacca tcggcatcgg cgggaacggg gcagccgtcc ggcgcagcgc
     6361 acgacatcgt catcgtcaca ttttgcgcga cccgcggccc gcccgagttc accagcatca
     6421 ggtggaacgt cttgctcttc gcgagcggca ccgtcaccgt ccagctgttg ttggtcgcgc
     6481 cctgtttcac gtcgatgagc ttgtcgtagg tgcctgcccc ggtcatgttc caggtaatcg
     6541 gtcctccgtc gagccgctgg gcaatttgta gcttgggcgc ctgacttgtc gctgtcggat
     6601 cgatgttgtt gcagaccgcg cccgccgcca gcgtggtgaa gtcaaacgtc accgtatctg
     6661 ccgaagctgg catgtcgatt ttccactgcg catgtccggg ccatactttt ttcgcgtcgg
     6721 aatcgaggca cagcatcttg tgcggcgtct gtacccgaat gatccgatcg cacagactga
     6781 gcacgccctg cgtcgtcatc gcgtctgtgg tcttttggct cgcatccgcc ccgagcgtcg
     6841 ccgccagctt gccgacttca tttacgacca gcgtgcccat ttcggtcatg gtcgtgccca
     6901 tcaccgatcc ttcaatcgcc gccagcaccg cctgatcaaa cgcttggcgt ttctgcaccg
     6961 ccgtcgcgct cgtatcgagt ggatcacccg tcactgcttt gcgcgcccgc cacagcgcgc
     7021 ccgaccacgg attggcatct tcatgcactt caccgacgcg gtcgtttgag cagtgaaggt
     7081 tgttgtcgac gtcgcgcagg cccacggtgg ccaggccaaa gttctgtttg gcgtattctc
     7141 cgacggggct attgccggtt gtcgccgatg aaaagaaatc ggcaaagcct tcgttcatgg
     7201 cgccgggatc atcgttgagc ccgtaattgt ctatgccgac cgaaccaacc aagcgatttc
     7261 tcgtcgagat caccgcgtgg gtgaactcgt gatagatgac gtcggcatcc atcgagtagt
     7321 tggccttggc gcccatgcca aacgcgatca tgtcgccagg gacaccgagg atgaagtagc
     7381 tgtagccgcc gcccgaatca cccggtgagt aaaaggcgtt ttcaaacggg ctcagcgcaa
     7441 tgttcggatt ggtcgctttc gccgcgttcg tcagatccgg catacacagg ttggtcaaaa
     7501 gcggaaacgg ctttggcgtc gcgccggtcg aaattcgcag cttgaaatcg gcgattccca
     7561 atgccccaaa cagcgtgcgg aagcgcgtgt agatgttggc ggtgtgccag tacatgtgcg
     7621 cttcaccaaa cgagttcttg ttggcatcgt ttagcgtcgg acagtgaccg tcgtttgccc
     7681 cgatgagcgg caggaactgg ttgtagtcgc ccgacgtgtt cgaaagcgac tgccccggcg
     7741 tacagaggtg cagcttggtg gtgcctcccc ctggcagccg cgtcgtaaaa tcgtccacac
     7801 agttgagccc cttgatcagc gtgctggtca tgccgcgcgc acctgtgccc gtcggtgcaa
     7861 acggtcctgc atcgccgctg ggaatcccaa actgatcgat gttgctggtc gtccctgggt
     7921 tatagcgata cacgccaaac tgattcagga agttcagtcg ctcgatccgc cgaatcacct
     7981 ggccactttg cgcgtcgacc aggtagtcac cgtgctcgag cgccgacagc tccatccgcg
     8041 acaccaaata cgcgagtcgt cggttctggt tgtcgatcat cagcaccggc tcgccgatct
     8101 ggcccggacc gagcgcccgc accgcttgct gcgcgtcttg cggtgatatc ttcggcaccg
     8161 gatcaatttt ggcgagcgcg tccgtcggta ctacgtcacg gaacagccgc tcgacttgtc
     8221 catccgtcga aatccgcagg accagctccg aaccccagac cggaagaccg tggtgctgct
     8281 ggcgatagcg cacaacgtgt ccagttccga cggggatcag ctcgggttcc gacagctctt
     8341 gcggaaccca agtcggcgac gactggcgaa gctggacaag atagcgctcg gctaccgtca
     8401 gcggtgcggt tcccggcgaa gctgctgtga gcgagcccag cagcagtcgc ggggttccat
     8461 ccatcgcgcg cgaaacaacg ttcggtgaaa tggtcgtggt ggagccgctg gcggcgtgcg
     8521 cctggctcgt cggaaccacc aaggccacag gcacgggcag acagacagca agcgaggcaa
     8581 aggcacagag ccgcgaaata cgcagggttc gcatgggatg ctccttgcac gccggagttt
     8641 cggcgcagtg tcagccttgc ttaccagaaa tttttggccg acggagggac aaaatcacaa
     8701 gtcgctggag cggcgattcg gtatttttcg cttgcgtcta attcacttcc gaagaaaatc
     8761 cctgcggccc atccctgtgg ctggatgggg tctgtggagc caacatcgtg atttccgatc
     8821 tctctcgcgt ccgtaacatc gggatctccg cgcatattga cagcggcaag accacgctca
     8881 ccgaacgaat cctcttctat accaagcgca ttcacgccat ccacgaagtc aaaggcaaag
     8941 atggtgtcgg cgcaaagatg gacagcatgg atctggagcg tgagcgcggc atcaccatcc
     9001 agagcgcggc gacctactgc tcgtggaaca accaccacgt caacatcatc gacacgccag
     9061 gacacgtcga cttcaccatc gaagtcgagc gcgcccttcg cgttctcgac ggcgccatcc
     9121 tggtgctgtg ttcggtggcc ggtgtccaga gtcagtcgct gaccgtcgac cgccagatgc
     9181 ggcgctatcg cgttccgcgc atcgcatttg taaacaagtg tgatcgcacc ggcgccaacc
     9241 cgctgcgggt caaaaaccag ctgcgcgaaa agctcggaca caacccggtg ctgctccagc
     9301 tgccgatcgg acttgaggac aagttcgagg gcgtggtgga cctcgtcacc atggaggcga
     9361 tcaccttcac gggcgacaac ggccaagaga tccaccgcaa tccgatcccc gccgacatga
     9421 aatcgcaagc ggacaaagcg cgcgaggaaa tgctcgatac ggtgtcgatg ttttcggatg
     9481 cgctgaccga agcgatcctc gaggaccgcg tcacgccgga gctgattcgc gaagcgatcc
     9541 gccgcggcac gctcgacatg aagctgacgc cggtgatgat gggatcggcc tataaaaaca
     9601 aggcggtcca gcttctcctc gacgcagtga cctactacct gccctgtccg tcggatgtcg
     9661 tcaacgaagc gatcgacatc gacagcccgg acaacaagaa agtcacgctc cagtcggatt
     9721 caagccttgc gacggtggcg ctggcgttca agctcgaaga cgggcgctac ggccagctga
     9781 cctatatgcg tgtctaccaa ggcgcgctgc gcaaagacga tgtcatccac aacacccgca
     9841 ccggcaaaaa ggtgaaggtg gcgcggctcg ttcggatgca ctccgacgac aaggaagaca
     9901 taagcgatgc cggtcccggc gacatcatcg cgatgttcgg cgtcgactgt aactccggcg
     9961 ataccttcac aagcggcgtg cagcttgcga tgacttcgat gtacgtgccc gacccggtca
    10021 tcaagctggc gatcaagcca gccgaccaga aggcgcagat gaacatgtcg aaggcgatcc
    10081 agcgcttcac caaggaagat ccgacgctgc gctgcgaagt cgatcccgaa agtggcgaga
    10141 cgctgctggc gggtatgggt gagcttcacc tcgacgtgta catcgagcgc atgaagcgcg
    10201 agtacaacgc cgttgtgcaa gtctcgccgc cgcaggttgc ctaccgtgag acggtgacgc
    10261 agcgtgctga ctataactac acccacaaga agcagacggg tggttcgggt cagtacggta
    10321 aggtcatggg ctacatcgag ccgtgtgccg aaaaagactt cgagttcgtc gatgaagtcg
    10381 tcggtggtac ggtcccgcgt gagttcatcc cgagcgttga aaagggcttc cgctcgatgg
    10441 tggcgaaggg tcagctcatc ggcttcccgg tcgtcaacgt ccgcgtggtc ctcgcagacg
    10501 gtggttcgca cgcggtcgac tcgtcagata tcgccttcca ggaagctgca cgaggcgcct
    10561 ggcgcgaggt ctaccaaaaa gccaagccgc agattcttga gccgatcatg aaggttgcgc
    10621 ttgagggtcc aagcgagcac cagggcaaca tgctttccac cctgatgtct cgtcgcggta
    10681 tgatcattgg cacgaccgaa gcagacggta tgtcgacaat cgaagcggaa gtgccgctgg
    10741 ccgaaatgtt cggctatgcg acgacgctac gctccgcgac gcagggcaaa gccgagttct
    10801 cgatggagtt ctcgcgctac gcccctgtgc cgggtggcct cgccgaggaa ctgattgcca
    10861 aggttgccaa ggcgaaagcc gcagagaaga agaagtagcc aggaggccca tcatgtatcg
    10921 caaagaagtg aacgaacgga gcccgctacg gattcttgag aaatcgagcc acggtggtct
    10981 gggccgcggc aacctcggtg ttgccatggc gcgggccggc gtcggcaaga cggcctttct
    11041 ggtgcatgtg gcgctcgatg acctgatgcg ggatcgcaag gttctgcacg tctcgctcga
    11101 ctcgcccgtc gaccacgtaa agagctggta cgacgcggtg tttcaggatc tggtcaaaac
    11161 agccgatctt gcagaccacg cggtgacgca agagctgatc aacaaaaacc ggatcatcca
    11221 ggcctacagc ctgcatggtc acggtggtgc caatgagttt tcgatcgcga agctcaagca
    11281 ctcgatcgag ctgctgtcgc agcacgcgca gtttgtgcct gatgtgatca tgatcgatgc
    11341 gttcgactgg tcgcgggcga cgctggaaga agcggcagag ctgcgggcgc tcgccaagca
    11401 gttcaacgtc gagctgtgga tgacggcact gacgcatcgc caccagacgg gccccgaagt
    11461 gcctgatcgg gtgccgccgc cgtgtgatcg gtttgccagc ctgattagcc ttgtggtgtt
    11521 cctgcagccc gttgacaaga acctctcggt gcggctgctt aaagatcacg acaacgaggt
    11581 ggtctgcgac acgcatctgg tgctgcaccc cgacacgatg cggctcgttg acgaccgcgc
    11641 gccgagctcg ccagccagtg atctgccgcc gtcggcctat acgctgctgt cgggtggcgc
    11701 gattggggcg gaatcggagt tcggtgcctg cgccgagcgc tacggcctga aggaagaaaa
    11761 ctactcattc gagggacacc cagtggcgcg gacgcgcggt ctgcggctgc tcacgaatga
    11821 agaactgcgc gatggtgatg tgagcctggc atatgtctct gcgacgatgc atcggcccta
    11881 tcaccagaat ccgacgctgc gcaaggtgct ccagagcatt tggcaccagg tcaattcgtc
    11941 gcaggaagtg ttcatcatcg gcgtgatcca gcgcgacgac accgtcaagg gtggcacggg
    12001 ctgggcggca gagctggcac gtcaccaagg caagccgctg cacgtctacg accaagagcg
    12061 tcgctcgtgg tttacgtgga atcacacgac ccgtcagtgg caggcgtcga agccggtcat
    12121 tacccacacc cgcttcaccg gcaccggcac gcgcagcctc agcgatgatg gtcgcgcagc
    12181 gattcacgag ctgttcgcgc agagctttgg cgcggcgcgc acgggtcgct cgggcccgat
    12241 caaggcctag tgatgcgtgc ggggcagccg cgcttgtgca aactgctgcg cgctgtgctg
    12301 cccatcgtcg ttgtcctgct tgccttcgcg tttccgcaga ccgctttttc ccatcaatcg
    12361 agtgtggtgt ttcttcggct tgtgccggaa cgacacaccc tgcatgtgac gctacggatt
    12421 gcaaacagcg atctgtttga agcgctcggt ctgcctgccg accatgtggt cttgcccgaa
    12481 gacgtccacc gccagcggga acagctgacg gcgtatttgc tgggccgtgt gtccgtcgca
    12541 aaccttggcg ttggctgtcc gggtcggcgt gaacagagtg catttgaacc ccggcaaggt
    12601 ggctttttcg cggtcgaaac gctggcgttt gagtgtccac gcgcgcttga aaaatcgacg
    12661 ctgcgctacg atctgttttt cgacctcgat ccgcggcatc agggcatggc gcttgtcgag
    12721 ctgcccggtg ccccgcgcga atacattttt cgcgaaaaca gtcgcctgct cacgttggat
    12781 cagccgcgca cggttcttga ctatgcgctc gactacctgc ggcttgggat tgagcacatt
    12841 ttcaccggat acgatcacct ggcatttgtg atcgggctgc tgctgctcgt cggtagcctg
    12901 tggcgcaaga catcgccgcg gcacgcgctg ggctacatcc tgcgggtggt gacgggcttt
    12961 accgtcgcgc actcactgac gctcatcctg tctgcgctgc gatttgtcag cctgcccagc
    13021 cgatgggtgg aatcgctgat cgcgctgtcg attggttatg tcgccatcga aaacattgtc
    13081 ggcgtgaagg tccagcgacg gctctggctg gcgttcggat tcggtctggt ccacgggctc
    13141 ggctttgcat cggtgctgcg cgagctgggg ctaccgagtc gcggtctggt ggtgtcgctc
    13201 ctgtgcttca acctgggcgt cgaggtgggg cagctgacgg tggtgttgct gctgctgcct
    13261 ttgctcgcgc tcatgtcgcg gtcgaaatgg tatccgtccg tgatgatccg cggcgcaagc
    13321 tggattttgc tcgcgttgtc gctgatctgg tttgtcgagc gcgcttttga tatgcagtgg
    13381 gaacgaggcc gcgcggcgat tgcgtggaaa cgagtgtcgg gcgacgatcc gactgcattt
    13441 gctagacacg aaatccggtt gacgaccaca gccaaccgct cgtaacgtaa aaacccaagt
    13501 tcgcaccgac gaagtgcata aaggagcctg accgacatgg ccacgatgat caccgaggat
    13561 tgcatcaact gcggcgcctg tgagccggag tgcccgaatg aagccatctc cgagggagag
    13621 gatctctatg tgatcgaccc gaacctctgc accgagtgcg ttggtttcca tggcagcgaa
    13681 gcctgccagg cggtgtgccc ggtggagtgc tgcatccccg acccgaacaa tcgggagagc
    13741 gaggaaacgc tgctcaaccg cgccaagaag ctccacaccg gcgaaaagtt ccccgagctg
    13801 cacgaactga acgcgaaaaa ctcgctgttc agaaactctg cccgcaagca ctaggcaaaa
    13861 agcgtcattt tcgcggcaaa gtttcttttc ctggccgcgt cctccaccta agatcccatt
    13921 gtctgggctg actcctcgtc gagaggttct ggtccgccct ggcagcagat tcacaaaaag
    13981 ggatcgcatg cctgccgtta cgattgatgc caatgaagcc gtcgccaacg tggcgcatcg
    14041 gctaagtgaa gtcatcgcga tttatccgat cactcccgca tcggcgatgg gcgagctggc
    14101 cgatgagtgg tcgacgcatg gccgcaagaa cctgtggggc gaggtcccgc gcgtcgtcga
    14161 aatgcagtcc gaagccggtg ccataggggc cgtgcatggc gcgctgcaag cggggtcgct
    14221 ggcgacgacg tttaccgctt cgcagggtct tttgctgatg attcccagca tgttcaagat
    14281 cgccggcgag cttctgccga tggtcttgca cgtcgcggcg cgcacggtgg cgacccacgc
    14341 gctgtcgatt tttggcgatc actccgatgt gatggcatgt cgatcgacgg gctttgcgct
    14401 gctgtgtgcc ggatcggtac aggaagccca ggactttgcc gccctggcgc atgcagcgac
    14461 gctccagacg cgggtgccga tcctgcattt tttcgacggt tttcggacct cgcacgagat
    14521 cgccaaaatc gagccgatca gcgacgatac attgcgcagc ttgatacccg aaaccgggat
    14581 tgctgcggtg cgcgagcgtg cgatgacgcc ggatcgtccg gtgctgcgtg gcacggcgca
    14641 gaaccccgat acgtttttcc aagcgcgcga ggcctgtaac ccgttttatg aagcgttccc
    14701 ggcggcgctg aagcagcaca tggcgcgttt tgccgatctg accgggcggc gctacgagct
    14761 gttcgagtat gtcggccatc ccgaagccga tcgggtcatt gtgacgatgg gttcgtcgtc
    14821 agaatgcgtg catgaaaccg tcgagtattt gcaggcgcag ggtcagcggg tcggacttat
    14881 caaaatccgg ctgttgcggc ctttttcgct cgtcgatttt gcccgtgtgc tgccgaagac
    14941 ggtgcgccag atcgcggtgc tcgatcgtac caaagagcct ggtgccgtcg gtgatccgct
    15001 ctatttggat gttgtgacgg cgctttccga agcggtttcc gacgggcttt atccccacct
    15061 gccgaaagtc atcgccggtc gatacggttt gtcgtcgaaa gaatttacgc cagcgatggt
    15121 cgtcgcggtg ttttccgagc tggcacaagc gtcccctcgt cgccatttca cgattggcat
    15181 caacgacgac gtcagcaagc gatcactcga ttttgacgat tcgctgggca tcgagcccgc
    15241 cgacgtggtt cgagcggtgt ttttcggtct gggatcggac ggcaccgtcg gaagcaacaa
    15301 aaacaccatc aagatcatcg gcgaagagac cgaccacttc gcgcagggct attttgtcta
    15361 cgactcgcgc aaggccggtg cggtcacggt gtcgcacctg cggtttggcc cgaggccgat
    15421 tcgatcgacc tacaaaattc ggcgcgcggg ttttgtcgcc tgtcaccagc cgcagcttct
    15481 cgatcgcttt gaaatggccg agcttgccga cgagggatcg acgtttttgc tcaatgcgcc
    15541 gtggccagcc gatcgggcgt ttggattttt gccgcgtgaa gtgcagcagc ttttgattgc
    15601 gcgcaagatg cgctttttcg tgatcgacgg acagcgcgtc gccgaggaag cgggcctggg
    15661 gcggatgatc agcacggtga tgcagacctg ctttttcgcg ctttcgggtg tgctgcaaaa
    15721 agaagtcgca ctcgacaaga ttcgccaggc ggcgcaaaag tcctacggta agcgcggcag
    15781 cgaagtggtg cgcagaaacc tcgcggcgat cgatcgcacg ctcgacaact tgttcgaagt
    15841 cgccgtcccc gagctgccgt cgacgcagcg ctcgcgtccc gacatcgtgc ccaaaactgc
    15901 accggacttt gtgcagcgcg tgacggcact gatgctggcg gggcacggtg attatctgcc
    15961 ggtgagcgcg tttccggtcg acggaacgtg gccgcttggc actgcgattt acgatcggcg
    16021 cagcctcgcc aaagagatgc cgacgtggga ttcgcgggtt tgcattcagt gcaacaagtg
    16081 cgtgctgatc tgtccgcacg cggcgattcg ttcgacggtc tgcgatgaaa aacagctcgg
    16141 tgatgcgcca gcgggctttg tcgtcgtcga ttacaaagaa cgcgacgcgc tgccggggca
    16201 aaaataccgg cttcaggtcg cgcccgacga ctgcacgggc tgcgggctct gtgtgcagtt
    16261 ttgtccggcc aaagacaaga ccaatccgcg ccacaaagcg atcgacatgt cgccggcaga
    16321 accgctcaaa cccgtcgagc gcgagacgtt tgcgtttttc caaaagctgc ccgcgcctga
    16381 tcgcaagacg gtgcatctcg atgtcaaagg cagtcagttt ttgccgccgc tgttcgagtt
    16441 ttcgggggcc tgtgcgggct gcggtgagac gccgtatctg aaactgctga cgcagctttt
    16501 tggcgatcgg ctgatggtgg ccaatgcgac cggctgttcg tcgatttatg gcggcaactt
    16561 gccgacgacg ccgtggagca aaaacgcaga cggacgcggg ccggcgtggg ccaactcgct
    16621 gttcgaggac aacgccgagt ttgggctggg tttccgactg tcgattgatc agcacaccgc
    16681 gtatgcacaa agtctgctgc gcgggctttc cggtgaactg tccgacgagc ttgtgacggc
    16741 gattttgacc gcgccgcagg acagtgaagc gagcttggcg gcgcagcggg agcgggtcgt
    16801 cgagctgaaa aatcggctgc agcggatcgc ccgacctgaa tcggcgctgc tgcaagcgct
    16861 ggccgattat ttggtcaaaa agagcgtctg gatcgtcggt ggcgatggct gggcttacga
    16921 catcggcttt ggtggtctcg atcacgtcat tgcttcgggg accgacgtaa acctcttggt
    16981 gctcgacacc gaggtctatt cgaacaccgg cggacagcag tcgaaatcga caccgatcgg
    17041 cgcggcggcg aagtttgcgg cagcaggcaa agagctgccc aaaaaagacc tcggcatgca
    17101 cgcaatgatg tacggccacg tttacgtcgc gcgcgtcgcg atgggggcca aagacgcgca
    17161 cacggtcaag gccttccttg aagctgaaag ctatccgggt ccgtcgctca tcatcgcgta
    17221 cagccactgc attgcgcacg ggtacgacct gcaaatgggt gccgaacagc agcgcctggc
    17281 cgtcgattcg ggcgtgtggc cgctgtatcg cttcgatccg cgccggcttg atgttggcga
    17341 gccgccgctc aagctggacg cgacgccggg caaagccaaa gtgcagcagt acatggccaa
    17401 cgaaggccgc ttccggatgg tcgagcttgc cgatccagcg cgctacaaac agctgctgaa
    17461 agcggcagag cgcgacgtgg cgatgcgaca ggcgatgtat cagcatctgc aggggttgca
    17521 ggtgccggta ccgggcgcaa acaagcctgc ccaaagcgac aaaccagcga cacccagcga
    17581 caagccgacc ggggcaggga gccactagcg atgaaccttg cgacaagcta tttgggactc
    17641 aagctcgcgc atccgatcct gccgggtgcg tcgccgctcg ccgatgatct cgacagcgtc
    17701 aagcggctcg aagatgccgg ggctgcggcg attgtcatgc gctctctgtt cgaggaacag
    17761 gtggcgcaag agtcgctcgg cgcgtatcag catctcgacg gacatggcga tctgtacgcc
    17821 gaagcgcaga gctattttcc gtcgaccaac gtgtttgcac ttcgcgccga tgcgtatttg
    17881 gaacagcttc ggcggattcg tcagtcggtg gcggtgccgg tgattgcatc gctcaacggc
    17941 acgacgccgg gcggctggct cgattacgcc aaacagatcg aacaggcggg ggcgcacgcg
    18001 ctggagctga acctctattc gctgccatcg tcgcccgagc actcggcgca tgatgtcgag
    18061 cgcggccagc ttcaaatcat cgccgaggtc gcccgcagtg tcaggattcc catcgcggtg
    18121 aagctgtcac cgttttatac cagcctgccg cacttcgtcg ctgaagtggc ccgaaccggc
    18181 gcgcgtggcg tggtgctttt taatcgtttt taccaacctg acatcgacat cgaaacactc
    18241 gacaatgtgc cgcagcttca cctgtccgac tcgtcggagc tgctgcttcg gctgcgttgg
    18301 ctggcgattg tttcgccgcg gacgccgttg tcgctcgctt gctcgggcgg ggttcactcg
    18361 gccaccgatg cggtgaaagc gctgatggcg ggggcgcacg cggtgcaggt cgtttcggcg
    18421 ctgctgcagc acggtccggg tacgctgcgc aagatcgtcg acggcgtttc gcgctggctg
    18481 accgaacatg actacgagtc gatggaccag cttatcggca gcatgaacca cgcgcgctcg
    18541 cccaatccgt cggcctacga gcgagccaac tacatccgtc tgctcagtag ctttcatccg
    18601 caagactgac gctgctcgac gggaaatccc gagatatcaa aaaaattcgc gcaaaaattt
    18661 gcgctcaatt cctgtgccca tagcatgggc tgcgtgtctc tttcgacgga tgtcacacat
    18721 gggattgctt tgcgcacaat tgcgcatagt gcgccgctgc ctatccagac agggcgcgac
    18781 ggagtccttg ccgacgatcg agaacaagcc gcgtcgtgga atgaagcaga gcgcagcgtt
    18841 cttcgacatg taagtctttc ggatagcgaa tctatcttca cgaaagactg gtcagatgcc
    18901 gcgctgatcg gcgatgctga tgagcttgtg agcgagcaaa cagcgcagtc tccagcacgt
    18961 ccgtttgtga agtgggctgg cggcaaacgg cagcttctgc cggatttgct gcatcgggtg
    19021 ccaagctcgt ttcgtcatta tcacgagcct ttcgtcggtg gtggcgcgct ttttttcgcg
    19081 ctgaccgaac aaaatcgact ggatggcaaa ggcgtccgac tgaccgatat caatcgcgaa
    19141 ctcatcaaca catatcagat ggttcgggat cgagttgaaa cgctgatagc gctgctctcg
    19201 acgtttaaga acgacgaaag cttttactat acgattcggg cacttgagcc ttcacagctc
    19261 gacgaagtgc agcgagctgc gcggtttatt tatctgaaca agacctgttt taatggactg
    19321 ttccgagaga accgcagagg acagttcaac gtcccgttcg gtcgatacaa acaagcacaa
    19381 ttttgcgcac caaacgactt gcgggccgcg agtcgtgccc tgcgcggtgt ctgtttggaa
    19441 gtcgcaggtt ttgatgaaat cgcgatgagc tgccagccgg gcgatttcgt gtattgcgat
    19501 ccgccctatg ttccggtgag ccgaacagcg tcgtttacaa gctacaacgg cggtggtttt
    19561 ggcgacgagg atcagcagcg tctggccgac gtggttcgcg tgctgggcga gcgcggtgtg
    19621 catgtacttt tgtcaaactc ggcggtgccg ctgacctacg cgctgtatcg cgagctgcac
    19681 atcgaggaag tgctggcgcg acgggcgata aactctcgcg gcgatcgtcg aggacacgtt
    19741 cccgagctgc tggtggctgc cggaccgctg gcatccgagc tgctggctcg tcgtcgccct
    19801 ggtgcgccgg cttcgcttgc caagtgaccg cctggcggcg aacgattccg tcgttttttc
    19861 ttttggcgtc gctgatacac tgcacgcgat gacgcaccgg gttcctgttc ggctgctctg
    19921 tggtccgctg tgcggcctgc ttggccttct gtcgtcgtgt acgctttccg agtcgtatct
    19981 tgtgccgagc aaagcgatcg agcttgtgca cagccagccg ccggattcgc ggcagcgggc
    20041 gcagcttccg gcgctgcgtg agtcggatcg ctccgcggcg ctgattcgtt atcgggcact
    20101 cgaatggagc ggcgagacgc tcaatcgggt ggctgcgacg ccgatgacga tggcgcgggt
    20161 gcgggtcgcg aaagtgaatc cgctgatgat tgccggcgga acgatcttgg cgctttcggt
    20221 gccgcacctg gcgctcggga tggcagcggg acttgacctg ccgccggggg aaaccaagcc
    20281 cgcagatccg atcgggatgg gcatcggtct gggtcttggt ggactgcatg tgttcgtcgg
    20341 tggcttgctg atggcgctgg gcgaacggcg tcccaacatc gaaccgaccg agcctgcgct
    20401 ggtaaaggcg tatttgcgcg gtgaaattcc gacggcgact gcggcccaag aagccgtgac
    20461 ggaaaccggg gaagccgcgc ctccgcctga tccgccgcgg cgctcgacct ataacgtccc
    20521 gctttcaccg gacgacggta agtagtaaga cctgggcacc atgatctccc gctacacccg
    20581 tcctgagctg gccgcgctgt ggagcgagtc gcggcggctc gcgctttttc ttgaagtgga
    20641 actggcggcg actgccgcgc tcgaggaagc ccaatcgagc cccattccga ctggcaccgc
    20701 gatgcgtctg cgtcagctgt cggcgcaaat gggaccgctc gacccaagtc gcgtatcgga
    20761 aatcgagcga cgcacccagc acgaagtctt ggcgtttttg gaacatgtgg aagaacgtct
    20821 gggccccgag gcgcgctggc tccactttgg acttacttca tcggatgtgc tcgataccgt
    20881 gctggcgctt cagctgcggg aagcgacgtc tatcatcatt cggggcatcg acgaacagct
    20941 tcttcctgcg ctgcgacgac aggcgacggc gcatcagcag acgccgatga tcgggcgcac
    21001 gcacggaatt tttgccgaac cgatcgccgc cggcctggtt ttcctcggta gctatgctga
    21061 aattgagcgc gccaaagccc gaatccagcg cgcccaacgt gcgatcgccg tcggaaaatt
    21121 gtcgggcgcg gtcggcgtct acggcagcgg ctgcgtcacc ccagacgtcg aagaaaaagc
    21181 gctgtcgagc ctcgggctgg tggcagaaac cgtcgcgacc caagtggtgg cgcgcgatcg
    21241 tcatgccgag ctgttgtggt cgctgtcgat gctgggggct gccgtcgagc gcctggcggt
    21301 gaacctgcgc cacttgcagc gcagcgaagt gtctgaagtc gaagaagcct ttgcgccggg
    21361 ccaaaaaggc agctcggcaa tgccgcacaa aaagaatccg atctctgcgg aaaatctctg
    21421 cggtctggcg cggctgctgc gggccaatgc ccacgccgca gtcgaaaaca tcgcgctgtg
    21481 gcacgaacga gacatctcgc attcgtcggt ggagcgcgtg atcatgccgg atagcacgac
    21541 gctgtgcgac tacatggtgc agcgcgccgc caagctggtg ttcgggcttt cggtgcgcac
    21601 cgggcagatg gatcgcaacc tggcccaaag cggtgatctg tatgcgtctg aaaaagtgat
    21661 gctccagctc gtgcgcagcg gtctttcgcg acagtcggcg tatgaactgg tgcagcgtca
    21721 tgcgctggct tgcgtcgccg agcggggcag tgcggacgcg ccgtcgtttc aggctcgcct
    21781 ggctgccgat ccgcagattg ccgataggct gccgacatcg gtgctggctg cctgtttcga
    21841 gatcgcgacg cagcttcagc atgtgccgca gattttcgct cgcacgctcg ccgactgacc
    21901 gaatcgccgc ttgcgccgac ggaacgcttg gcgaagtcgc gaaaatgcgg tcatctaact
    21961 agcgaaaact cttgatagaa aaaatgattc ccccttcttt gctagcatcc gcatgctgtg
    22021 caagaatcct tatctgaggc gacagtagcc tcactgagac cctaatggac gactttcact
    22081 gctccttctg tggcaagagg cgtcgcgaag tccgcaagct gatcagcggc ccgcgtgtct
    22141 tcatctgcga cgagtgcgtc tcgctttgca acgacattct ctcgaaagag gacgcgcagg
    22201 aaaggccgcg ctatcccaag cccgtcgaga tcaaagacga acttgacaag tacgtcgtcg
    22261 ggcaaggccg cgccaaaaaa acgctcgccg tctcggtgta taaccactac aaacggctga
    22321 tgtcgcgagg ccgcgccggt gacgtcgatg tccacaaggg caacgtgctt atcatcgggc
    22381 cgacgggctg tggcaagacg ctgctcgcgc agacgctggc caagaagctc gatgtgccgt
    22441 ttgcgattgc cgatgcgacg acactgaccg aagccggcta cgtcggcgaa gacgtcgaaa
    22501 gtgtcatcaa agcgctgtat cgcaacgccg gtggcgacat cgaaaaagcc cagcgcggca
    22561 tcgtctgcat cgacgagatc gacaaaattt ctcgcaaggg cggtggcccg tcggtcacgc
    22621 gcgatgtcag cggcgaaggc gtccagcagg cgctgctcaa gctgctcgaa ggcaagcacg
    22681 cgacgattac ccccgacgga gcgcgcaacc gtccgcagca agagcttatc caggtcgata
    22741 ccaccgacat cttgtttatt tgctgcggtg cgttcaacgg tctggaagac acgattcgtc
    22801 gacgggtatc cgagcgcgga atgggctttg gggcacgaat ttcggcccgc aatgatgaag
    22861 acaaagacgc gctgcgcgcc caggttcgca ccgaagactt gatcaagtac ggcatgattc
    22921 ccgaattcct cggtcgtctg ccgatcatcg tttcgtgcga ctcgctcgac gaaaatgcgc
    22981 tggccgaagt gctgtggaag ccgaaaaacg cgctgaccaa gcaatattcg cggctgttcg
    23041 agatggaagg cgtcaagctg cgcttcaccg acgatgcgct gatggcagtg gccaaggaag
    23101 cgtaccgtcg caagtccggc gcccgcggcc tgcgcgcgat catggaagaa gtgatgctgg
    23161 aagtgatgta cgaaatcccg tcgatgacag gcgtgcgcga gtgcgtcatc aacgccgacg
    23221 tcatcaccag ccgccagcag ccgcagctca tccgcgaaaa aaaagccagc taagctagcc
    23281 ccgccactcg ctttttccga cgaaaaattg cattccggtg cgccgttttg ggcgcctgcg
    23341 tctttgctac tggtcgtctc gacggtagcc caagatgcgg tgcggacctt cgcccacgtc
    23401 ataggcttcg tcgacaaaag gacggagctg cgctggcagg gccggaatcg gctcgccggg
    23461 gctcgtcatc gcgaaaacaa agccttgcgg tgcggcgaga tccgtcgcga agggcaacca
    23521 cttgggcaag ggcagggcgg cgcgcgaaac cacgatgcca aaaccggctg gcagtcggct
    23581 ggcatcgtcc gcgaagacgt caaacttaag ccccagttcc cgtcgcagcg tcagtaaaaa
    23641 cgccgctttt ttgccaattc gttcgctcag cgtgacctgc aagtccgggc ggcacagcgc
    23701 acagagcaca cccggaaaac ccgcgccgct gcccacatca acaagcgtct gcgccggcag
    23761 cttgctcgat tccggcaggc gaccgaccag cgccaggctg tcgagaaagt gccgccggac
    23821 aatctgttcg cgacccacga ccgccgtcag gttgatcttg cgtccccact cttcgagcag
    23881 ctcggcgtag cgaatcagcc gtcgtgcgtc ttccggcgac agacagacac cttgccgggg
    23941 cgctgcgctg accagcagac gttcgagcgg cgacagcttt tcaatcgaac taaccgcgga
    24001 gtcctgcatc cgtactgctc agatccgtga cgccgccatc gctcacactg ccatcggaac
    24061 ttccgccatc gctgcttccg ccatcggtta caggcgcgca gcagcggctt ttttccgtcg
    24121 gacaggccgt cttgtcggtg ccgggccgat gctggggaaa cgacatcaca ccgctcgcgc
    24181 agtcttgacc ttcggcgacg cagtagctgc tggccgagca ctctggaccg cgtgcgacgc
    24241 agaccatttc tggcgacagg cacagggcaa ccaggtctgc cgacggggat gtgtagtttc
    24301 cgcagatcgc cgccgaatcg agcggaccgg gcgccgcact tcccaacttc catgtgcccg
    24361 acgtagcccc agtttcgtcg gcagtaaaga tcaaccgaca ggatgcgccc gtcgcacagc
    24421 tatcacactg gccttgcagc ggtccggact tgtggaccag ataacaggcg cggggaaagc
    24481 tacagcgacc caagatcgcg gggtcttggc cgggcgtcgg cgtggtcgtc gtgcagtaaa
    24541 ttccgagcac gctcccggcg acaagacaca gcgacagagc aagtcgttta ccgcgtcgag
    24601 gatatgtgtg tcgagtcgtc acttggttcc gctgggtttg tcggtgctgt tgccaagcga
    24661 cagaagcagc gctgaaagaa gctgctgcgc ctgcatcatg tagtcactga cggctttggc
    24721 ggtgcgcacc gtgtctttgg ccttcagtcc ggcgatgaca tcccgatggc acttgcgctg
    24781 aacggtggcg ggcggcagaa cctgcgcaaa aaacgccgcg tgcgccgaaa ccgccatccg
    24841 aattgtgttg atgagcagtt gaaacacccg gttgcgtgcg gtgcgcgtca gctcgacgta
    24901 aaactcaaag tccagcttga gcatttcgtc gggcgagaca tcggcatcgg cttttttcgc
    24961 aatctcctcg agccgggtga gatcatcggc ggtggcacgc tcggcggcca gctcggccac
    25021 atcgcgcgca aagatccgcc gaaattccag cacgtcgaac agcacatcgg cattgggaat
    25081 cccgtcagcc gttccggtca ccaggtgcga gaccagctcg acgcctgcgg tttccataaa
    25141 cggaagtacg cgcgttccgt cgccctgacg ggtcttaacc aaacccatct gctctagcga
    25201 tttgatggct tcgcgcagcg acgcgcgatt tacaccgaga tcttcagcga gcttgcgctc
    25261 gggagggagc ttgtcaccaa taccgtacac gccacgcaga atcaggctgc gcagctgttg
    25321 aacaatttct tcagcgactc gttgcttacc aattggtttc agcatcggcg gcagggtagc
    25381 aggtttgcgg aaaagtggaa cagctacaaa aacccgctgc gcaaggtcga taagtggaca
    25441 gaccagcgtg ccatttcgcc gttgtcggag cggatagaat cgaggcgggc tagcaggcga
    25501 agctcatcgt gccggtattg ttggtctcgt tgcactcgct ggtttgacct gtgcccatac
    25561 cgttgtcgtc ggcgatgatt tggacggtgt ggctgccgct ctggctcagc ggtgcgctgc
    25621 agcttaccgt cgagcagagc ccgggggcca gcgcctcggc ggtgagcgca gtgcagagca
    25681 ggattccatt ttcgtaaaac gaaatcggcg tgccatcgac gacgggcgct gttccgcgat
    25741 tgcagatcga cgtcgagagc tggaccgtgc tgccctgaca gaccgcggtg cccttgttgt
    25801 ttggtgccgt gacatcaccg gctgccgacg agctgagctt gccttggaca ttcatgcgga
    25861 agttgttgtt gcccggcaca tcccagttgc gctgccactg gctggtgcgc ggaatgccgc
    25921 ctttgtcgtc ggtgttggtg atcgagtacg tgtgctggtt ccagagcgga cgcgacgcga
    25981 cccagcgatc ggcgacgtcg ccgaacacgc gaatgccggt gcgcttgcca gtaaacgcgg
    26041 cttggcaggt attgccggtg ccagggacgc cagcgatggg aggcttgcag acaaagccgc
    26101 cgccgacgct cgcggtgttg cactgggtat cggcggtgca gcggcagtag ccctgatcgc
    26161 agccgatgcc gtttggacag tcggtgctgg cggtgcagcg caggcctttg aagatcgggt
    26221 cgaccgccgg gcaagccacg ccgcagtttt cattggacgg gacgatcagc ttgctgcgga
    26281 agtttccaac cacgtcagcg acgacgggat attcgttcca agtgcaggac gagtgggctt
    26341 gcgaaaacag gatttcgccg gttttgcccg aatagatgcg ggcgaaacac tcgtcggcgt
    26401 agaccgcttc ggcggtgcca tcgccttcaa aatcgaacag cgacgatccg gtgatgttcg
    26461 agctgacgtc ttgcgacagg ttgctccaca gcaggcgatc ggtgcgtccg ctcgggcaga
    26521 acttggcttc ggctccggcg acgcagtcgg gatcgaacac ggtgtaagaa ctggagccag
    26581 cggcggcaaa ctcggcgcgt ccatcgttgt cgaagtcgcc aatcgttggc gggccaccac
    26641 ccgtcgatcc agggaggctg agcgggccaa agacgatcgc gccgtctttg gacacaatcc
    26701 aagcgcgtcc gctcatgatg atggcaattt cggcgattcc gtcggagata ttgcggtcga
    26761 tcgtggttcc gttgggcacc ccgaaatcag cgacggcaac ataggcacgg gcttgactaa
    26821 gtcccggcgg cagcggcaga tcccgatctt ttttccactt cgaattggcc ttgtcccacg
    26881 agtaaatcgc cgtcggtgag accaagtttg gaatgccgtc tttgtcgatg tcggacacga
    26941 cagagaactg cccagtgtct tggccggtgg tgcgaagatt gaccgaggag tcgatcagca
    27001 cgccgctcgc gctgtacacg aagccttcgg cgatgatttc gggcctgcca tcgtcgtcga
    27061 gatcgtggat cgccgggcca gtccattcgc acagaccggc aaacggattc gagtccgccc
    27121 cggcgccggt tttcgatgtc cacagcgtca cgaacttgtt ttgcgcgcgg tcaaagccaa
    27181 aggcgacgag tccgcccgtc gcagaatacg caacgatgtc tgccgagcta tcgccgttga
    27241 gatcgccgat ggctggggtg gtcgcaccgt ttatcggcgt cgcaatgaca tactgctgat
    27301 tgcagcttct gccgtcgaga acgcgcagga ttccgtaatt gccgctgtaa agaccgcagc
    27361 ttccgtcggt gccgctgtag gtgttgacca ggatcgacgg acgggtagtg acaaactcgg
    27421 aaccgcgacc ggcaaagttg aagtccatca ccagcggcgt cgagagcact tgcttgtgat
    27481 tcggatacgg gtccgttgcc agcggttttt gccattcaca aaaaatctgg ggcgatagca
    27541 gcccggcggg aaccaggcgt tcacaggttg ggttgttggg gtgatcggcg ctgtagggtg
    27601 cgcagcggtt gttcaaacag tatgaatcac cagggcagtc actatcgacg gtgcagcgcg
    27661 tttttgaaac acaaatgctg tcgatacatt cctcattgtc ggcacaggcc ggatcgcaca
    27721 gcccggcccc gtttcccact tcagaacccg agcagccggt catcgctgcg aaaagcgcga
    27781 tcatcgacga tgccagcagc gtgtttaacc ccgagcgtcc cttcagcttg cgaaacattg
    27841 gtttgcctcc ccatcggatc atcggctgtc ccgagtgcca aaatccggaa ttaaaaatgc
    27901 cgatattcta tttatgatag cgcgattgct tgttcgcaca ccaaagccgg cctcgtcgta
    27961 tgacgctcgg cggcgctggg gcgagccgac ttgccaaaac gccgcccttt cgctcgaagc
    28021 gccgacattt tcttgggggt tggcggtcgc tgtgttacct atcgaagaca cctgtcgtgg
    28081 ccatccagcg cgatggttgc gatcgaattt tgtgtattta gctacatgac ttgcgctaaa
    28141 gtagcggtca aactcacctc tggtcgtctg aggcacgcgg tcttcctgac cgatgcccac
    28201 gggcccgcag tcgagcttgc aatggtctaa ccagcatgtc gaacgccgaa c
//
LOCUS       DAORGN010000049        27058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_690_length_27058_cov_6.410547, whole genome shotgun sequence.
ACCESSION   DAORGN010000049 DAORGN010000000
VERSION     DAORGN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27058
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_27058_cov_6.410547"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PKL17_07700"
     CDS             <1..997
                     /locus_tag="PKL17_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK44650.1"
                     /translation="AKIREIVGRSPAMLEVYKLVARVASSQSTVLVIGESGTGKELVA
                     RAIHNHSPRASGPFVAVNCTAISESLLESELFGHVKGAFTGAQHAKRGYFEEAQGGTL
                     FLDEIGDINAKMQAQLLRVLQEGEIRRVGSTEPVHVDVRVVAATNRDLDEEVQKGRFR
                     EDLYFRINVVTIRLPPLRERPEDITLLVEHFLAKYGDREGRHSIGVADEAMELLCRYG
                     WPGNVRELENVIERALALSKSGIVLPSDLPIDVRGSEARSVPRSGGSLVEDRPTLAEL
                     EQRYINLILQQEGGNKKRAAEVLGIDRRTLYRSLERGVPDRSERFDREPSDDKDG"
     gene            1033..1671
                     /locus_tag="PKL17_07705"
     CDS             1033..1671
                     /locus_tag="PKL17_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNK44651.1"
                     /translation="MSTLSLSVCQHPLLCARGFVTEFPSPLASMPSPDWLKSGLSLSA
                     SAPLSSSDALRQAVRDLLRHGGYKPTGRGKPASEYLLRAASEGGLGSINLAVDACNVV
                     SLHSGIPVSVVDLGRAKPPLSIRIGAAGETYVFNASGQTIDIAGLLVLCDAEGPCANA
                     VKDAQRTKTHDGTSQTLSILWGCTSEREQTSRALDYYLELLHKLGAKTQVVL"
     gene            complement(1829..3391)
                     /locus_tag="PKL17_07710"
     CDS             complement(1829..3391)
                     /locus_tag="PKL17_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44652.1"
                     /translation="MHTVGRTAWLSPLRHLVRFGVLLVLLVLGACGDNAQNQSDGPLI
                     RLHVTPVDGSVDKAVLSVAATDAMGVSKTNMSTFTAAPYDLLGVTFPVGTRGATSFQV
                     DLYSGSCLVGTGTATLTIDSDNVVDLNVTIKAIPLCGNGVTLTVQIAKIVSGNGTVSS
                     NPPGINCDDTGKNCTLIVAKNTPITLTATNTGGAMSSFNGWSGGGCSGTGNCTVPMDQ
                     DTTVQAVFTSCHGWCKETIPASVTTSLNGVGGSGASNVVVAGNSGTVLTWDGSSWKTI
                     GLPGGQTPNLRGVAGKVGFGAVYLVGDTAKIFKWTTTMTPLTTTSTSNLNSVVIAANG
                     LGYVVGSVGESLLIGTNGAVASGQLAIGTTTMNSICATTGGDFVVGGNKATVNAVAGL
                     WDGNKKTTAQMATGPAVPGNIYATLCGGTYHYAAGDGGVIVRRSSTAGQDNAWMTQTT
                     NVTTAIRGMWSSGDTFIIAVGDGGTILQYDGVAWKTMTSNTTANLRAVWGSSPTNIFA
                     VGDGGTVLHYTP"
     gene            complement(3473..4387)
                     /locus_tag="PKL17_07715"
     CDS             complement(3473..4387)
                     /locus_tag="PKL17_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44653.1"
                     /translation="MKIRFRQLGLYRKTVWLIVALLGALWTLGEKSANAASVVISEPQ
                     LEGELSPSEKTQLRSALESALKSQQLAPIAQTDLEATLSGEAQLQGCHTELCYERLGR
                     LLDGQMVVRYRVRRGEGKTPVAWRFSVEVLDVEVGAFGAKLSEDCPSCNDKQAAEKLG
                     DLAKRAIIESVARPRGTLDIRSEPSGAAVFVDGTELGITPYKRTTFAGPHKVVLRHVG
                     YRSEQVDAVIEDGQKQRIERTLTVGTDPTKVVVIEKTPVYKKWWFWVALGGAVAVAAG
                     VTAGVVIATRPAASTDRTPPSNNLSFSF"
     gene            4547..5146
                     /locus_tag="PKL17_07720"
     CDS             4547..5146
                     /locus_tag="PKL17_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNK44654.1"
                     /translation="MRTSVLLALLLCAAPALAQTPAAAPTAAPAPAAAGVTLSISQKD
                     SSITYRLIHKLHKFDGVSKQVEGKGRILPDGKAQVMVRAKVESFDSGNANRDSHMKET
                     VEAARFPQVELKASGEAALPASFPATVDKTFNAELSFHGVKKNLSIPVKLTYQSATSI
                     KAEAHLMLSLDEFKIERPSLMFVKIDDAMQVDIALTFSP"
     gene            complement(5169..5858)
                     /locus_tag="PKL17_07725"
     CDS             complement(5169..5858)
                     /locus_tag="PKL17_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44655.1"
                     /translation="MRFSYAHMLAAGMIHRLWKSALFGLVGIAAVSCYRPTYDGPYLC
                     DPNNKGSDCPDGWSCNSGQCLAPGQTPHQNGICSGSGMLLAMARGDQVWACSGSFAQG
                     AASTLCIDQPGVHVCGAGPHDEALISLIDCDTVQGFFLSLASVSIGPFPICDPGPAMP
                     HALLGCGTAPSVKSLPGPDCHGLHHALGCFDTSGWSCDPPLGGVSAAHTDSASNPGGV
                     LCCSGGMIPKR"
     gene            complement(5858..7057)
                     /locus_tag="PKL17_07730"
     CDS             complement(5858..7057)
                     /locus_tag="PKL17_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44656.1"
                     /translation="MIGSALTLWLLLLAMPAQMLTPGAQRVDSLHVLSRDPALGDEVA
                     RAARRYLHRPTQVRKDSESEPQPDSDGIVRLLVQTDSSTEHGLRCLLTLSQGTRSLKR
                     SLQLRETPNHIDLAEAIAIELPALLSLLSEQGIAQSPNPAPVSATIPPLRRASPPMGN
                     PMPARSAVLAKSAARSHPARTASEPSEASATSPTETENPTTEPAARATTKTEPDPVLA
                     MRSPAPTQAVSTIPASIPKDSVPTDSGSKDSAANSPEEKTPKETVAIQASDAKTPELF
                     VAASKSDPVKIRRPAGAVGLLIGGGALLLAGVGTGIETLLVAKQVSQPTADGHFDKQL
                     DQQGKTLSAVTIALDSVGGAALLAGSVWLYVHTQRAAKLGASKRRVAVAPLVQKAGMG
                     MLSEVRF"
     gene            complement(7054..8229)
                     /locus_tag="PKL17_07735"
     CDS             complement(7054..8229)
                     /locus_tag="PKL17_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNK44657.1"
                     /translation="MSSQPKDGAKEEELRPLRSLLGSPNASQLAPDERLLAEAFHLVS
                     EDSHPDSETASFDKLEGAAQRLARRRRWRYHLWEAWRDMGNVRLLATSAAVLVVISVA
                     VLSLRGNSSSDDRGGLSGLYQRITQPSSFREQLTLRSEKESRSPLRCGAELFVIGQVD
                     VEQKERFRPNIKLARGQVRVHVPPLPSGGKLVVETPDAEVIVHGTRFAVTREEGSSET
                     SVRVEEGLVEVLPKGGNRAAVFLRAGESLTVPSLAQYQKTLGAKVGQLIEEGRCDAEA
                     DALLRSYLDSGMLSFPSENAVAESAARYHLGSCAADRGEVENAVSLFESVAKGDGIRA
                     DNALARIAQLRAEHDRKSGQLGWQRYLVRFPNGLHRESAHRYLKESEERPQTESPPR"
     gene            complement(8222..8833)
                     /locus_tag="PKL17_07740"
     CDS             complement(8222..8833)
                     /locus_tag="PKL17_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK44658.1"
                     /translation="MNKPIEFLPLGASAERELVAGLCRGEAGCYRQLYDRFAPRVQRL
                     LLRIFADPQLARDAVQSTFLIVFEKIDRFDGRSSLLTWITRIAINEAHGQRRKSARRP
                     PERHDDGPLPPSPEDQHGDKELQKKLMELVQTLPEEKRTALLLFEIEGFAVAEIAEIT
                     GEPRGTVLARLSRTRAELQKELSAWCAQEPNAANRQVRSKEYV"
     gene            complement(8949..9674)
                     /locus_tag="PKL17_07745"
     CDS             complement(8949..9674)
                     /locus_tag="PKL17_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44659.1"
                     /translation="MKTSLSLGFVSALLFGLGIGCGDSLSLGSDVMAITGGSLSGEQL
                     ALPDCDGAGARNHAGRTQLSELDELTSLLALSADQVAQIQPILDATRAKAEDIRAQVQ
                     AGTLSKEEGRAQIKALHDAQNAQIMALLTPDQQTKFASMRDHHHGPFDLTKLKDAIGL
                     SDDQVSQISALQDAAQSKISDLHAQVEAGTLSADDAHTQIEQIRQDTMAQVKAVLSAD
                     QQAMLDKILAAHGRKGPGGPLRR"
     gene            9908..10957
                     /gene="era"
                     /locus_tag="PKL17_07750"
     CDS             9908..10957
                     /gene="era"
                     /locus_tag="PKL17_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNK44660.1"
                     /translation="MKSPSKNKQAEEKSATHGRVGFVALMGRPNVGKSTLLNSILGEH
                     LSIVSPKPQTTRNRILGVKNIKPRRSDAGTKPAVQLALVDTPGLHLTSIKGRTALNRF
                     MVEEAQQAVDGVDAVLLVVNLKLPDGKIDDDFVPSLAHADEQIAEQLVALGKPLIVAL
                     NKTDLVSDKRLFLPLIEALAQKLPGAPIVPISAMLGDGVDVLVDEICAKLPEGEPLFP
                     QEVLTDRPERFLVSELVREQVFLATQKEIPYAVAVTIDGWQERIADHGKKQGERIGVQ
                     IDATIHVEKPGQKKILVGERGQMIRNIGTLARQQIGELLGCGVHLQLFVRVDEDWSQT
                     PSGLRKMGYEMEARR"
     gene            10954..12450
                     /gene="der"
                     /locus_tag="PKL17_07755"
     CDS             10954..12450
                     /gene="der"
                     /locus_tag="PKL17_07755"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNK44661.1"
                     /translation="MKSAPRPVVDRSLPVVALVGRPNVGKSTLFNRLTGNHHAIVEDM
                     PGVTRDRRYGQCEYDGRLFRVVDTGGIDFGAEGSIAVGMRKQAELAIAEADVVLLIID
                     GQKGLLPDDRDIFVKLRRGGKKILIVANKIDGAKHESGLVDLYGLGSGDVFGISAAHG
                     RGMPDLLDAIVDGIPKAEPVAVHEPPAFADVEDDADDEPALSEEELARIDAAIDSESE
                     EDPADEGPATVSEADLLRDQIRIALVGRPNAGKSSLVNALCGEERMLVDPTPGTTRDP
                     IDTPILHNGQRFTLIDTAGIRRRVRVHEASDRIAMTMAERAIERAEVAVLVIDASAGI
                     GEMDAKIAGLVNDAGRVLLIVLNKRDLVPPAEVKAIEAELDRKLQFVPWAHRLWVSAQ
                     TQRGVLKILEEATRCYKSFASRVKTSALNRFFAGIVEKHPPPLFQGKPIRLYYITQAQ
                     TKPPTFVLSVNYPEGMHFSYRRYLQNQLRESFGFVGTPIRLVARAKTK"
     gene            12463..13383
                     /locus_tag="PKL17_07760"
     CDS             12463..13383
                     /locus_tag="PKL17_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNK44662.1"
                     /translation="MRSVRFGLWLGIIGLVGCDGIQKVAQDGPLSCHSDDDCAAQPMQ
                     KACHAGFCADPGCPLGTAYVGAGPYSRGCDPIDPDCELGAQPLHTVTIDHGFCVSVTE
                     LQVQQYRQCVAAGACTKAPDLRCTQDLATWTESPDANEALPLTCLLWSEADAACRFLG
                     GRLPTEAEWEKAARGRDARKYPWGNVQPLGCGSAVNYDGGGSCPGRPWSASSASRQGA
                     QLFSAARAVDMAGNVWEWVTDYYSPLAYRDCATGCIDPLGPLSGTLRSRRGGGFQSSQ
                     WKELTTFWRDFHTPESARSDSQGARCIFPK"
     gene            13392..14501
                     /locus_tag="PKL17_07765"
     CDS             13392..14501
                     /locus_tag="PKL17_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44663.1"
                     /translation="MSFSWAVTAHRTLWKGLLGLSVWAQLAFLAHAETKPGLSLSVRE
                     VSTLPGVYAGQLAFAPDGKTWAAGQQREISLFDGDKPIRTLTPVWVDSDADLHFLADG
                     KKLAAGGRIYSLPDGKELFAVSANRAVHERGFEIDAATISPDFAKCVAWVKYHPSRCC
                     RESGHRDAPAHKPASPVFVIDTQSGQTQPLPIEDGGFGEYRALAASSRYFVVGGVANK
                     ATVFDRKTAKPAATLSEDGAFYAFRFSADEKTLAAIHLGRFLIVYDGDRFTKRTKIEV
                     LPDGKWLTALAISPSLPIVAVSGWDGNLRLYSLASSDVGKLLWSGPLGQASAIAFSPK
                     GNELLVAVSNPNRIVRLAVSVVAGDGQRDRSLGHR"
     gene            complement(14451..16217)
                     /locus_tag="PKL17_07770"
     CDS             complement(14451..16217)
                     /locus_tag="PKL17_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44664.1"
                     /translation="MRLSRTLPLAVLVCIFAEPAQAKDSELYGSVVDGLRLGLTVTPS
                     RSVLPSDVEVRITLQNTGSSRKSIPISTCHSISWASYARLLVRTDGGKTYRMAFSGMV
                     DTADLHPHIPLELAPSETLTVTRRLTELAEHFPAEDADLGLAEKLLSAKQIELAADFF
                     SSEKGVPKLRSGQAIQRFGLSPIDKPLSTGCVAQIATSHHVACALNRAGTPYCWGDTE
                     PGFNPDTKRHEVSIPRAITRLRKGVRSLSFGTSELCTITTEGVFCVRGGNDSVSVNRL
                     TLPYRIYGLPPSVARLWTGSADQCAQTDAGALWCWGRSFLSAPPGSVGTEWRAKEHSV
                     LGHDVTSVVMGAQHACAQKRDGSVFCWGENKAGQIGNGTLVGTEDPTLVSGFGGPIRQ
                     LAAGHNFTCGLREDGQVLCFGKNEYGALGKDIQTAFSRPFVRSDLGKENVALFAAQNQ
                     LCAQKLDGKLVCMGVVAQDSQRLTTQTPQLVDRIPSELSAVSFGPRDTCTLSKQGQVH
                     CYGETPGAMKLPDEPSPPAGAQPIIGLSDVASVQLGHSFFCALTRAGKVSCWGDGSGG
                     QLGAGKLTMAKRPIALSIPCDD"
     gene            complement(16292..16642)
                     /locus_tag="PKL17_07775"
     CDS             complement(16292..16642)
                     /locus_tag="PKL17_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44665.1"
                     /translation="MRLCLAVLLGLLTWFGTIPARADVSPPPSCGSTSSADITACSGK
                     MAGDACSFANGTKGSCASLRCVTDAGTTQLACVATGASSPGCSFSGTMESGAAVGMLL
                     LGTLAAALLRRRER"
     gene            16829..17590
                     /locus_tag="PKL17_07780"
     CDS             16829..17590
                     /locus_tag="PKL17_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNK44666.1"
                     /translation="MNVVPTRSFHSGWIAVSLGVIFSTAIAAQSWVKVRLHRDHSIEV
                     TGSAKRRIVSDLIEWKADVATEGKDRFAAYKSLHDHVERTLAYLKEQGIAETELRVSS
                     VFTEEMSETEYIGKGDDRIERKIPKGFRTSQTISVSSKEIAKIEKVSREVTQLMSSGV
                     PVTSESPSYYYTQLGALKIEMLAEAAKDCRIRAERILSSTGGAHLGRLRRADMGVINI
                     NPANSTEVSNEGNSDVSSLEKDILTIVHCSFDLSE"
     gene            17638..18153
                     /locus_tag="PKL17_07785"
     CDS             17638..18153
                     /locus_tag="PKL17_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44667.1"
                     /translation="MRHTMLFAVSVFLFACGPTKGPVTTVGASIFTSDVITVVAENHG
                     GGFVPPPPAGSACALGAAKYTLTVGSKFFDWTRCVSSGMNPYTIAKGSRTLTDAEYKN
                     LVPYLENLTVVAGDGLCGADKGTLDVTITTPSGQQTYGDSFYSCSIKDKPLVRTDALD
                     RLFSQLYTLAM"
     gene            complement(18168..25505)
                     /locus_tag="PKL17_07790"
     CDS             complement(18168..25505)
                     /locus_tag="PKL17_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44668.1"
                     /translation="MVLFGFSAKGFAAAPTVVTVAASGITSSSAVLNGSGNPGGEATT
                     GRFRYSATNPGTCNDTFGTRVPATSGTDLGNGSATVPYSITTTGLTPGTTYYFCAIVT
                     NASGTAYGSVLSFTIPAQPLVTTSAATSITSNSATINGTANPFTLSTTGWFRYSTTNP
                     VTCDDSFGSRIPSSGGQNLGTGITPYAYAQSLTSLSPGTTYYFCAIAQNSLGTSVGTV
                     QSFTTPSMAPTVSTNSASNLSRTGAQLNGYANPGGDATTAWFRYGPASPGTCNDTWGT
                     RTPATGGTSLGSGTSNASFSQTVSGLLPGVTYYYCALAKNSQGLSWGSVQTFITPSAP
                     TATTVFATYITNTTAQLNGLGNPNRAATSGWFRYALTDPGTCDDKFGSRAPLSGGTAL
                     GSDIYDQSYSQSISGLSPATTYYYCAIVQSSEGTAYGTILTFTTATVPVATTNPATGI
                     SATTATLNGAGNPNAATASGWFRYSASNPGSCNDSFGTRTPSSGGTNLGSGAASMPFT
                     YSLSGLTPGTTYYYCAIVSNTYGNAYGTVQTFATPANAPTVTTSGAYNLSRTSATLSG
                     YGNPGGDATTGWFRYSTASPGTCNDSFGTRAPATGGTALGSGTSNVTFTQAITGLAPA
                     TTYYFCAIVQNSGGQVVGSVQSFITPSAPTAATASATNLTTSSAQLNGSGNPNRASAT
                     GWFRYSIANPGTCDDKFGSRSPLTGGTALGNDIYDQTFAQSISGLSGGTTYYYCAIVQ
                     SSEGTAFGTVMSFTTSGIASATTNAASAVTSTTATLNGSGVPNGATASGWFRYSTTNP
                     GTCNDSFGNRAPSSGGTNLGAGTTSSPFAQTISGLSPGATYYYCAIVSNTYGTGYGAV
                     VQFTPLTMAPTVNTSGASSLTRTGAVLSGYANPGGDATTGWFRYSTASPGTCNDSFGT
                     RAPAMGGSALGSGNTNVSFTQTISGLAPATTYYYCALAQNSLGMAMGSVQTFITPSAP
                     TALTAAASNITDTTVLLNGSGNPNRAYTTGWFRYATTNPGACDDKFGSRAPVTGGTNL
                     GSDIYDQPYNQTLSGLSPATTYYYCAIVQSSEGTAFGALLSFTTATAPTATTNPATSI
                     TSTSAILNGSGTPNGGTSTGWFRYSATNPGSCSDSFGTRAPSSGGTNLGAGTSATNFQ
                     QGIGSLSPGTTYYYCAIVTNSYGTSFGAIVSFVTPATTPTVSTSGASNLTRTDALLSG
                     YGNPGGSATVGWFRYSTASPGTCNDSFGTRAPATGGSAMGAGTSNVSFSQAISGLSAA
                     TTYYYCAIAQNAQGIVFGSIQSFITPSAPSALTTAAGTIYDTSAQLNGSGNPNRAYTT
                     GWFRYATTNPGTCDDKFGSRAPLSGGINLGSDIYDQPYYQTVSGLSPATTYYYCAIVQ
                     SSEGTAFGAVMSFTTASPAVAVTGMPASVTSTSAMLSGTGTPNGGSTTGWFRYSATNP
                     GACNDSFGSRIPSSGGTNLGAGFSPTSFAQSIGSLLPGTKYYYCAIAQNSYGIGYGAV
                     VSFTTPVQAPAVTTSGASNLTRTTGVLYGYANPGGDATTGWFRYSTASPGTCNDTFGT
                     RAPAMGGSPLGSGTSNVTFSQAITGLAPATTYYFCAIAQNSQGVAWGTVQTFITPTAP
                     TALTTPATTITTSSAQLNGSGNPNRGATIGYFRYAATDPGTCDDKFGSRAPTSGGINL
                     GSDIYDQPYYQSISGLSPATTYYFCAIVQSSEGTAFGTVLTFTTSAPATAATVAATSV
                     TPTTATLNGSGNPNGATATGWFRYSTSSPGTCNDTFGTRTPTSGGISLGAGISTVPFG
                     QLITGLSPGVTYYYCAIVQNAYGTGMGALLSVTTPAGPPTVSTDYPSGIATTLNGTAN
                     PNGSATTGWFRLATSSPGTCNDTFGTRIPTTGGTALGSGVVGVSYSNAATGLTPGTTY
                     YYCAIAQNAVGTAFGTIRSFVAPMPPTVNTLAATPVTATTAQLNGTANPNGNSTIGWF
                     RYSTSSPGTCNDVFGTRAPTSGGTSLGAGSSATPFGESISGLSPGTTYYFCAIAVSAY
                     GTVFGSVLSFTTPTTLRTPIKPSAPGGTTPPITPTPSDVDLLAQRIVTLDATSIGTQT
                     AILVGSANPSSEEMTGWFRLSSRDPGTCNDNFGIRGPQKGGAAVGASKIPVSFTQAMS
                     GLSAGTTYYYCAIGSTDKGHFFGAVKQFTTRAAAPKVTTSAPTDVTAQMAQVHAAIVP
                     SGDDTVAWFRYSAEPQESCNDSFGKRVPEHNQQSVGSGSREVVISETLYGLQRGTTYY
                     VCAIAQNSHGTTLGELVQVVPGERAPEVETENPAAVDSATATLAGRVREHQGGSRTWF
                     RLGEQNPAVCDDSFGIRVEARSLVVPSATSNLRFDTTPQPGDDTYRTSEPLTGLTPGT
                     TYYYCAAASGVGGVTFGAVRSFTTALPPPEVGTVTMSAQTAEGTVGCSVGGTASSRGS
                     VPAVLALLPLIGLLAARRRARRR"
     gene            complement(25852..>27058)
                     /locus_tag="PKL17_07795"
     CDS             complement(25852..>27058)
                     /locus_tag="PKL17_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44669.1"
                     /translation="HAFLCGAFSLQGTTLSVTLRLLAVDSGFQLFCRRWELPFGEALS
                     MCQKTAQEIAVALTSEVTAPQSDDVLDPETLNLYLKARQLYTGNDSSFFHHSVELLEQ
                     ALAQKPNSPLLLVGYAKACARAWFWGHSEDEHKAIRAAQRAVEVAKNQGPPYLALGAA
                     CYGTGDLPGSVRALRKGLGIAPQLADCHDLLGRILCECGPLSEAVAHLETASRLDPLS
                     TRPRLEMARLFAMTGAWKQVDSLLEQELNDPRAVPLWWMITGRMALWRKDPAWAKQKL
                     TMPGDESVGCQRGKRLLRVAASEDGSASLSDDEFRQLFEAGSSANPRRRVMLHQGRTE
                     LLCFEQKREAALLSLADCVEDGLCDVMWIDQCPLLELLRDEPRFVTLRQVVEARASQI
                     RAAMETPLP"
BASE COUNT         5095 a         7892 c         8568 g         5503 t
ORIGIN      
        1 cgccaaaatt cgcgaaatcg tcggtcgctc gccggccatg ctggaggtgt acaagctggt
       61 ggcgcgggtc gccagctcgc agtcgacggt gctggtgatt ggtgaatcgg gaaccggaaa
      121 agagctggtg gcgcgggcga ttcacaacca ttccccgcgc gcgagcggtc cgtttgtcgc
      181 cgtaaactgc accgcgatca gtgaatcgct gctggagtcc gagctgtttg gtcacgtcaa
      241 aggcgcgttt accggggcgc agcatgccaa gcgcggctat tttgaagaag cgcaaggcgg
      301 aacgctgttt ctcgatgaga ttggcgacat aaacgccaag atgcaggcgc agcttctgcg
      361 ggtgttgcaa gaaggtgaaa tccgtcgcgt cggcagtacc gaaccggttc atgtcgatgt
      421 gcgggtggtt gccgcgacga accgcgatct cgatgaagaa gtgcaaaaag ggcgcttccg
      481 cgaagacctg tatttccgca tcaatgtggt gacgattcga ctgccgccgc tgcgggaacg
      541 gcccgaagac atcacgctgc tagtcgaaca ttttttggcc aaatatggcg accgcgaagg
      601 ccgacactcg attggtgtgg ccgacgaagc gatggaactt ttgtgtcgct atggctggcc
      661 gggcaacgtg cgcgagctgg aaaatgtgat cgagcgcgcg ctggcgctgt ccaaaagcgg
      721 aatcgtgctg ccatcggatc tgccgattga tgtgcgcggc agtgaagcgc gcagtgtgcc
      781 gcgatcgggg ggaagtctgg tcgaagatcg tccgacgctg gccgagctgg agcagcgcta
      841 catcaacctg attttgcagc aggaaggtgg caacaaaaag cgcgctgccg aagtgttggg
      901 aatcgatcgc cgcacgctgt atcgcagctt ggaacgcggc gtgccagatc gcagcgagcg
      961 attcgatcgc gaacccagcg acgacaaaga cggctagatt ccttttggca aagcactgct
     1021 atagtgccgc gcatgtcgac gctttcgctt tctgtctgcc agcatccgct gctttgtgcg
     1081 cgcggttttg tgacggaatt tccgtcgccg cttgcgtcta tgccatctcc ggactggctc
     1141 aaatccggtc tgtctttgtc ggcttctgca ccgctttcct cgtccgatgc gctgcgccaa
     1201 gcggtgcgtg atctgctgcg ccacggtggc tacaaaccca ccggcagagg caagccagcg
     1261 tcggaatatc tgctgcgtgc cgcgagcgaa ggaggacttg gttccatcaa cctggcggtc
     1321 gatgcgtgca acgtggtgtc gctccattcc ggaattccgg taagtgtggt cgatctggga
     1381 cgggcgaaac cgccgctgtc gattcggatc ggggccgccg gggaaaccta tgtgtttaat
     1441 gcgtccggac agaccatcga cattgccgga cttttggtgc tgtgtgatgc agaaggtccg
     1501 tgtgcaaacg ctgtcaaaga tgcgcagcgg accaagacac acgacggaac cagccagacg
     1561 ctgtcgatcc tctggggctg tacaagtgaa cgtgagcaga caagccgcgc gctcgattac
     1621 tatctagagc ttttgcacaa gctcggcgcg aagacgcagg tggttttgta gcgcgatttc
     1681 gcgcggtaga ccatttggac tgtatccaaa ctagttttgt actgtatcca atatgaagca
     1741 aatcaaatgc ttcatattgg tttggcctgg gtggcgtggg aattgggcga tggggccgta
     1801 ggcgtgcgcg gctgcgcaag cggacagatc aaggcgtgta gtgcagcacg gttccgccat
     1861 ccccgacggc aaagatgttg gtcgggctgg atccccacac tgcgcgcagg ttggcggtgg
     1921 tgttggatgt catcgttttc cacgcaacgc cgtcatactg caagatggtg ccaccgtcgc
     1981 cgaccgcgat gatgaaggtg tcgccgctcg accacatgcc acgaatcgcg gtggtgacat
     2041 tggtcgtctg tgtcatccat gcgttgtctt gtccggctgt gctggatcga cggacgatga
     2101 cgccaccatc gccagcagca tagtgatagg tgccgccgca cagcgtcgcg taaatgtttc
     2161 ctggaaccgc aggaccggtg gccatttgcg ctgtggtctt tttgttgcca tcccagagcc
     2221 cagcgacggc gttgacggtt gctttgttgc ccccgaccac gaaatcacca cccgtggttg
     2281 cgcagatgct gttcatcgtg gtggttccga tggcaagctg acccgacgca actgcaccgt
     2341 ttgtgccgat gagcagggac tcaccgacgc tgccgaccac atagccgagg ccgttggcag
     2401 cgatgaccac cgagtttagg ttcgatgtcg atgtcgtggt aagcggtgtc atcgtcgtgg
     2461 tccatttgaa tatcttggcg gtgtctccga cgagatagac cgctccgaag ccgacttttc
     2521 cagcgacgcc gcgcaggttg ggagtctgtc cgcccggcag tccgatggtc ttccacgaag
     2581 aaccatccca agtgagcact gtgccgctgt ttccggcgac aacgacgttg ctcgcaccgc
     2641 tgccgccgac gccgttcagg ctcgtcgtga cgctggcggg aatcgtttct ttgcaccagc
     2701 cgtggcagct tgtgaagacg gcctgcacgg tggtgtcttg atccatcgga acggtgcagt
     2761 tgccagtgcc gctacagccg ccgcccgacc agccgttgaa ggacgacatc gcaccgccgg
     2821 tgttggttgc ggtcaacgtg atcggcgtgt ttttggcgac gatcagggtg cagtttttcc
     2881 cggtgtcatc gcagtttatt cccggcggat tcgacgacac agttccgttg ccgctgacaa
     2941 ttttcgcgat ctggaccgtc agggtgacgc cgttgccgca gagcggaatc gctttgatgg
     3001 tgacatttag atcgacgacg ttgtcgctgt cgatggtgag cgtggcggtg ccggttccca
     3061 ccaagcagct tccgctgtac aggtcgactt ggaagctggt cgcgccgcgc gttccgacgg
     3121 gaaatgtgac acccagcaga tcgtaaggtg ccgccgtgaa cgtgctcatg ttggttttcg
     3181 agacgcccat cgcatcggtc gcagccacgc ttaggacggc cttgtcgacg ctgccatcga
     3241 ccggggtgac gtgcaggcgg atgagcggac cgtcgctctg gttttgggcg ttgtcgccac
     3301 atgcaccgag cacgagcagc acaagcaaga cgccgaaacg gaccaggtgg cgcagtggag
     3361 acagccaagc agtgcggcct acggtatgca tcccatcctc ctacgtaaat gagtcgcgcc
     3421 gacggagatg cgtttgcggc gcaggactcg gggcttgctt ttggacacgg ccttagaacg
     3481 aaaaggaaag gttgttgctt ggcggcgtgc gatcggtgct ggcagccggg cgcgtcgcga
     3541 tgacgacacc cgctgtgaca cccgctgcca ccgcgaccgc accaccgagc gcaacccaaa
     3601 accaccattt tttgtagacc ggagtctttt cgatgacgac cactttggtg ggatcggttc
     3661 cgacggtgag tgtgcgttcg atgcgctgtt tttgaccatc ttcaatcacc gcatcgactt
     3721 gttcggatcg atagccgaca tgacgcagca ccactttgtg cggacccgca aacgtcgtgc
     3781 gcttgtacgg cgtgattccg agctcggtgc catcgacaaa caccgccgcg ccgctgggtt
     3841 cggaacgaat gtcgagcgtg ccgcgcggac gcgccaccga ttcgatgatc gcgcgcttgg
     3901 ccaaatcgcc gagtttttcg gcggcctgtt tgtcgttaca gcttggacag tcttccgaca
     3961 gcttggcgcc aaaggccccg acttcgacat cgagcacttc gacggaaaaa cgccaagcca
     4021 ccggtgtttt gccttcgccg cggcggactc gataacgaac caccatctgt ccgtcgagca
     4081 gccgcccgag ccgttcgtaa cagagctcgg tgtgacagcc ttgaagctgc gcttcacccg
     4141 acagcgtggc ttccagatcg gtctgggcaa tcggcgcaag ctgctgggac ttgagcgcgc
     4201 tttcgagcgc ggaacgaagc tgggtctttt cgcttggcga cagctcgcct tcgagctgcg
     4261 gttccgaaat gaccacactg gcggcgttcg cgcttttttc gcctagggtc cacagtgcgc
     4321 cgagtagcgc gacgatgagc cataccgttt tgcgatacaa acccagctgg cgaaagcgga
     4381 tcttcatgcc ggccccctca aaagacaatg cagagcgcat caaacagtca tcgtatccga
     4441 tcccgataat tttcaatagc tgggcgagga gttcgttatt ctgtgggcac tgatgaatcg
     4501 tagccttggc ttgcgtctaa acgacgagcc catctaagga gtccacatgc gtacctctgt
     4561 tctgcttgct ctcttgcttt gcgcagctcc ggctttggcg cagacccccg cagccgctcc
     4621 caccgcggca cccgcccccg cagcagcggg cgtgacgctt tcgatcagcc aaaaggacag
     4681 cagcatcacc tatcgactga ttcacaagct gcacaagttc gatggcgtgt ccaaacaggt
     4741 cgaaggcaaa ggccgcatcc ttcctgacgg aaaagcccag gtgatggtgc gtgccaaagt
     4801 ggagtcgttc gattccggga atgccaatcg cgattcccat atgaaggaaa ccgtcgaggc
     4861 ggcgcgcttt ccccaggtcg agctgaaggc aagtggcgaa gcggctctcc cggcttcatt
     4921 cccggccacc gtcgacaaaa cgttcaacgc cgagctgtct tttcacggcg tcaaaaagaa
     4981 cctgagcatt ccggtcaagc tgacctacca aagcgcgacg agcatcaaag cggaagcgca
     5041 ccttatgctg agtctcgatg agtttaagat cgagcgaccc agcctgatgt ttgtcaaaat
     5101 cgacgatgcg atgcaggtcg atatcgcgct cactttctcg ccgtaaccgc cgcagcgggc
     5161 ggcttgagtc agcgtttcgg aatcatgccg ccgctacagc acaacacgcc gcccggattg
     5221 ctcgcggaat cggtgtgggc ggcgctcacg cccccgaggg gcggatcaca cgaccagccg
     5281 cttgtgtcaa agcatcccag cgcgtgatgc agtccatgac agtccggacc gggaagcgat
     5341 tttaccgacg gcgctgttcc acaccccagc agcgcatgcg gcatcgcagg tccgggatca
     5401 cagatcggaa acggtcctat cgaaacgctc gccagcgaca gaaaaaatcc ttgtaccgtg
     5461 tcgcagtcga taagcgagat cagcgcttcg tcgtgcggtc cggcaccgca gacatgaacc
     5521 cccggctgat cgatgcagag cgtacttgcg gcaccttgcg caaagctgcc gctacaggcc
     5581 cacacctgat caccgcgtgc catcgcgagc agcattcctg aacctgagca gattccgttt
     5641 tggtgcggcg tctgtccggg ggccagacat tggccggaat tgcagctcca cccgtcggga
     5701 cagtcgcttc ctttgttgtt gggatcgcac agatagggac catcgtaggt gggccgatag
     5761 caggacaccg cagcgattcc tacaagtcca aacagggccg acttccacag tctgtggatc
     5821 attccggcag ccagcatgtg ggcgtaagaa aaacgcatca gaagcggacc tccgacagca
     5881 ttcccatccc agctttttga acaagcggcg cgacggcgac acgtcttttg cttgcaccaa
     5941 gcttggctgc ccgctgggta tgtacgtaca gccaaacact gcccgccaag agcgccgctc
     6001 ccccgacgga atcaagcgct atcgtcaccg cgctcagggt ttttccctgc tgatcgagct
     6061 gcttgtcaaa gtgtccgtcg gcggtcggtt ggctgacttg tttggccaca agcagggttt
     6121 caatgccggt tcccacccca gccaagagca gcgcaccgcc acctatgagc agtcccactg
     6181 cgcccgcagg tcgtcggatt ttgactggat cagattttga cgcagcgacg aagagttctg
     6241 gtgttttggc atcactggct tgaatcgcca cggtctcttt tggcgttttt tcttccggag
     6301 aattggccgc ggagtctttg gaaccggagt ctgtgggaac ggagtctttg ggaatcgacg
     6361 caggaattgt actgacagct tgggtcggtg ccggactgcg catcgccaag accggatcgg
     6421 gttccgtctt tgtggtcgcg cgggctgccg gttctgtcgt cggattttct gtctctgttg
     6481 gcgatgtggc ggacgcttcg ctgggctctg aagcggtgcg cgctggatgt gatcgagctg
     6541 cgctttttgc caacacggcg ctccgcgcgg gcatcggatt tcccatgggc gggcttgctc
     6601 tgcgcagagg cggaatcgtc gcggaaaccg gcgccggatt tggtgactgc gcgattcctt
     6661 gttccgaaag cagagacaga agcgccggaa gttcgatcgc aatcgcttcc gccagatcga
     6721 tgtggtttgg ggtctcgcgc agctgcagac ttcgctttaa cgaccttgtt ccttgcgaca
     6781 gcgtaagcag acaacggagc ccgtgttccg tcgacgaatc ggtctgaacc agcaaacgga
     6841 cgatgccatc gctgtccggt tgcggttcgg attcactgtc cttgcgaact tgggtcggac
     6901 ggtgcaaata gcgtcgcgca gcgcgggcaa cttcatcccc gagcgcggga tcacggctca
     6961 gtacatgcag agagtccact cgctgggcac ctggagtcag catctgtgcc ggcatcgcca
     7021 gcaaaagaag ccacagcgtc aaggccgaac cgatcatcgg ggcggagact ccgtttgggg
     7081 gcgttcttca ctctctttca gatagcgatg cgcagactcg cggtgcagtc catttgggaa
     7141 tcgcacaaga taacgctgcc aaccaagctg gccacttttg cgatcgtgct cggcgcgaag
     7201 ctgggcaatc cgtgccagtg cattgtccgc gcggatgcca tcgcctttgg ccacagactc
     7261 gaacagagac actgcgtttt cgacttctcc gcgatcggca gcgcagcttc ccaagtggta
     7321 acgtgcagcg ctttccgcaa cagcattttc cgacggaaaa ctcagcattc cgctgtccag
     7381 ataggaacgc agcagcgcgt ctgcttctgc atcacagcgt ccttcttcga tcagctgacc
     7441 gacctttgct ccgagcgttt tttggtactg cgcaaggctt ggaaccgtaa gcgattcacc
     7501 tgcacgcaaa aacacggctg cccggtttcc gcctttgggc aaaacttcaa ccaggccttc
     7561 ttcgacgcgc acgctggttt cgctgctgcc ttcctcgcgt gttacggcaa agcgcgttcc
     7621 gtgcacaatc acttcagcat ccggggtttc taccaccaac tttccaccgc ttggcaaagg
     7681 cggaacgtgg acccggacct ggccgcgcgc cagcttgata ttcggtcgga atcgctcttt
     7741 ctgctcgaca tcgacctgtc ctatcacgaa aagctctgcg ccgcagcgga gcgggctacg
     7801 cgattccttt tcactgcgga gtgtgagctg ttcccgaaac gacgatggct gggtgatacg
     7861 ctgatacagt ccggaaagcc caccgcgatc gtcgctgctg ctgtttccgc gcaaggacaa
     7921 aacggcgacc gaaatcacca caaggactgc cgccgaagtc gcaagcagac gaacatttcc
     7981 catgtcccgc cacgcttccc atagatgata tcgccagcgt ctccgtcgag ccaggcgctg
     8041 cgctgcgcct tccagtttgt caaagcttgc ggtttcggaa tcaggatggg aatcctcaga
     8101 aaccaaatga aacgcttccg cgaggagtcg ttcatccggt gcaagctgcg acgcattcgg
     8161 agacccaagc agactacgca gcggacgcag ctcttcttct ttggctccgt cttttggctg
     8221 actagacata ctctttactc cgtacttggc ggtttgccgc gttcggttcc tgcgcgcacc
     8281 acgcggacag ctctttttgc agctctgcgc gggtgcgaga cagacgagcc agcaccgttc
     8341 cgcgtggctc tcccgtgatc tcggcaattt cggccaccgc aaaaccttca atttcaaaca
     8401 gcaggagcgc ggtccgtttt tcttccggaa gcgtctgcac aagctccatc agcttttttt
     8461 gcagctcttt gtcgccatgc tgatcttccg gactcggcgg cagtggtccg tcgtcgtgtc
     8521 tttcgggcgg acggcgtgca ctcttgcgcc gctggccgtg cgcttcgttt atcgcgatgc
     8581 gcgtaatcca ggtcaaaagc gacgaccgac catcgaagcg atcgattttt tcaaacacga
     8641 taaggaatgt cgactgaacc gcatcacggg ccagctgcgg atcggcaaaa attcgcagga
     8701 gcagcctctg tacccgtggt gcgaagcggt cgtaaagctg ccgataacag ccggcctcac
     8761 ctcggcagag accggccacc agctctcgtt cggcggatgc tcccagcggc agaaattcga
     8821 ttggcttgtt catgcgctac ctggccatca tatcgtgggt tgtgggtgcc gcggtgcggc
     8881 gtttcccatc ccaaaccgtt agagcaagta ggcgggcggc tgattgcgtg gggcgaaaag
     8941 aaaatttttt accgtcgcag agggccgccc ggaccctttc ttccgtgcgc cgcgaggatc
     9001 ttgtcgagca tcgcttgctg atcggcagag agcaccgctt tcacctgcgc catcgtgtct
     9061 tgccgtatct gctcgatctg ggtgtgcgca tcgtccgcgg acagcgtacc tgcttccacc
     9121 tgcgcatgca gatccgaaat cttgctctgc gcggcatcct gaagcgcact gatttggctg
     9181 acttgatcgt cgctgagccc gatcgcatct ttgagctttg tcagatcaaa cggaccgtgg
     9241 tgatgatccc gcatgctggc aaacttggtc tgctgatccg gggtcaggag cgccatgatc
     9301 tgcgcgttct gtgcgtcgtg cagcgctttg atctgggcgc ggccctcttc tttgcttagg
     9361 gttccggcct ggacctgggc gcggatgtct tccgctttgg ctcgtgtcgc gtcgagaatc
     9421 ggctgaatct gtgccacctg atccgccgac agagcgagca gtgaagtcag ctcatccagt
     9481 tccgagagct gggtccgtcc ggcatgattc cgcgccccag ctccgtcgca gtcgggcagc
     9541 gcaagctgtt ctccgctcaa gcttccaccg gtgattgcca tcacatcgct gccaagcgac
     9601 agggagtctc cgcaaccgat tcccaaacca aacagaagtg ccgacacaaa accaagcgac
     9661 agagaagtct tcatggtacg tctccttcgg agcagccgtg tggagcgatg cagcgtccgg
     9721 accggaaaac ggacaccgca gcgcgggttc atttcgttcc atcgatggct gtaagagaga
     9781 gtccaaaccc cccgcgccga ttgcatccac gcgcattttt ttggatcgct tctgcctggc
     9841 aatcgtcgtt tgcggcgcgg ctgccgtttg cactgccggg aacggacgtg ctatgcaagc
     9901 gcccttcatg aaatctccgt cgaaaaataa acaggccgag gaaaagtcag ccacgcatgg
     9961 gcgggttggc tttgttgccc taatgggtcg tcccaacgtg ggcaaatcga cgctgctcaa
    10021 cagcatcctt ggcgaacacc tctccattgt tagccccaaa ccacagacca cgcgcaaccg
    10081 catcttgggg gtaaaaaaca taaaaccccg tcgcagcgat gccggaacga agcctgctgt
    10141 gcagcttgcg ctcgtcgata cgccgggtct gcacctgact tcgatcaaag gccgcacggc
    10201 gctcaatcgc ttcatggtcg aagaagcgca acaagccgtc gatggcgtcg atgcggtgct
    10261 tttggtcgtg aacctgaagc tgccggatgg aaaaatcgac gacgactttg ttccgtcgct
    10321 ggctcatgcc gatgaacaga tcgcggaaca gcttgtggcg ctgggcaagc cgctcatcgt
    10381 cgcgctgaac aagaccgatc tggtgtcgga caagcgcttg tttttgccgc tcatcgaggc
    10441 gctggcgcag aagctgcccg gcgcgcccat cgtgccgatt tcggcgatgc tgggcgatgg
    10501 cgttgatgtt ctcgtcgatg aaatctgcgc caagctgccc gaaggcgagc cgctgtttcc
    10561 gcaggaagtc ctgaccgatc ggcccgagcg cttcctggtc agcgagttgg tgcgcgagca
    10621 agtgtttctg gcgacgcaga aagagatccc ctatgccgtc gcggtgacga tcgatggctg
    10681 gcaggaacgc atcgccgatc atggcaaaaa acagggcgag cgaatcggcg ttcagatcga
    10741 cgccacgatt catgtcgaaa aacccggaca gaaaaaaatt ctcgttggtg aacgcggtca
    10801 gatgattcgc aacatcggaa cgctggcgcg gcagcagatc ggggaacttt tgggctgcgg
    10861 cgtgcatctt cagctgtttg tccgcgtcga tgaagactgg tcgcagacgc cgtcggggct
    10921 gcgcaagatg ggctatgaaa tggaggcgcg gcgatgaaat cggcacctcg tccggttgtg
    10981 gaccgctctt tgcccgtcgt ggcactcgtc ggtcgtccca atgttggcaa gtccacgctg
    11041 ttcaatcgct tgaccggcaa ccaccacgcc atcgtcgaag acatgccggg tgtgacccgc
    11101 gacagacgct acggtcagtg tgaatacgac ggacggctgt tccgggtcgt cgataccggc
    11161 ggcatcgact ttggcgccga aggcagcatc gcggtaggaa tgcgcaagca ggccgagctg
    11221 gcgattgccg aagccgatgt cgtgctgctg atcatcgacg gacaaaaggg tcttcttccc
    11281 gacgaccgcg acatctttgt caagctgcgg cgcggcggca aaaagatact catcgtggcc
    11341 aacaagatcg acggtgccaa gcacgaatca ggacttgtcg atctgtatgg attgggcagt
    11401 ggcgatgtgt ttggcatctc ggcggcgcac ggtcgcggaa tgcccgatct gctggatgca
    11461 attgtcgacg gaattcccaa agcggaaccc gttgccgttc atgagccgcc cgcgtttgcc
    11521 gatgtggaag acgacgctga cgacgagccg gcgctgtcgg aagaagagct ggcgcggatc
    11581 gatgcggcga tcgattccga aagcgaagaa gacccagccg atgaaggtcc agcgacggtc
    11641 agtgaagccg acctgctgcg cgatcaaatt cggattgcgc tggtcggtcg tcccaatgcc
    11701 ggcaagtcgt cgctggtcaa cgcgctgtgc ggggaagaac ggatgctcgt cgatccgacc
    11761 cccggcacca cccgcgatcc gatcgatacg ccgattttgc acaacggtca gcgcttcact
    11821 ttgatcgata ccgccggcat tcgtcgtcgg gtgcgcgtgc atgaagcgag cgaccgaatt
    11881 gcgatgacga tggccgagcg tgcgatcgag cgcgccgagg tcgcggttct ggtgatcgac
    11941 gcgagcgctg gcatcggcga aatggacgcc aaaatcgccg gccttgtgaa cgacgccgga
    12001 cgggtgctgt tgatcgtcct caacaagcgc gatctggtgc cgcctgccga ggtcaaagcc
    12061 atcgaggccg agctagaccg caagctccag tttgtgccgt gggcgcacag gctgtgggtg
    12121 tcggcgcaga cgcagcgcgg cgtgctcaaa attttggaag aagcgacgcg ctgttacaag
    12181 tcgttcgcga gtcgcgtcaa gacctcggcg ctcaaccgct tttttgcggg catcgtcgaa
    12241 aagcatccgc cgcctctgtt ccagggcaag ccgattcggc tctattacat cacccaggcg
    12301 cagaccaagc cgccgacgtt tgttctgtcg gtgaactatc ccgaaggcat gcacttttcg
    12361 tatcgccgct acctgcaaaa ccagctccgc gagtcgttcg gcttcgtcgg tacgcccatt
    12421 cgactggtcg cccgcgccaa aaccaagtga caatcggaac cgatgcgatc ggttcggttt
    12481 ggactgtggc tgggaatcat cggccttgtg ggctgtgatg ggattcaaaa ggtggcgcaa
    12541 gacggtccct tgtcgtgtca cagcgacgat gactgtgctg cgcagccgat gcagaaagcc
    12601 tgccacgccg gattttgtgc cgatccgggc tgtcctttgg gaaccgctta tgtgggggcc
    12661 gggccgtaca gccgcggctg tgatccgatc gatcccgact gtgagcttgg cgcacagccg
    12721 cttcacaccg tgaccatcga ccatggtttt tgcgtcagtg tgaccgaatt gcaagtgcag
    12781 cagtaccggc agtgcgtcgc tgcgggtgca tgcacaaaag cgccggatct gcgctgcacc
    12841 caagacttgg cgacgtggac agaatcgccg gacgcaaatg aagcgctgcc gttgacctgt
    12901 cttttgtggt cggaagccga tgccgcgtgt cgttttctgg gtggacgctt gccgacagaa
    12961 gcggagtggg aaaaggccgc gcgcggacgt gacgcacgca agtatccgtg gggcaacgtg
    13021 cagccgctcg gctgtggttc ggcggtaaat tacgacggtg gtggcagctg tccggggcgt
    13081 ccgtggtcgg cgagcagcgc gtcgcgacaa ggggcacagc ttttttccgc cgcccgcgcc
    13141 gtcgatatgg ccggcaatgt gtgggaatgg gtcacggact actattcgcc ccttgcgtat
    13201 cgcgactgtg cgacgggctg cattgatccg ctcgggcctc tttccggcac gctgcggagt
    13261 cgtcgcggcg gcggttttca gagcagccag tggaaagaac tgacgacatt ttggcgcgac
    13321 tttcacacac cagagagcgc tcgcagcgac agccaaggcg cgcgctgcat tttcccaaag
    13381 taggagggca gatgtccttt tcgtgggcag tgacggcgca tcgcacgctg tggaaaggtc
    13441 tgctgggtct ttctgtctgg gcgcagcttg cttttctggc gcacgcggaa acaaagcctg
    13501 ggctttcgct ttccgttcgg gaagtatcga cgctgcccgg tgtgtatgca ggtcagcttg
    13561 cgttcgcgcc cgatggcaag acgtgggccg cgggtcagca gcgagagata tcgctctttg
    13621 acggtgacaa accgatccgc acgctgacgc cggtgtgggt cgatagcgat gcggacttgc
    13681 actttttggc cgatggaaaa aagctcgcgg cggggggacg gatttattca cttcctgacg
    13741 gaaaagagct gttcgctgtc tctgcaaacc gtgctgtcca tgaacgtggc tttgaaatcg
    13801 acgccgcgac catttcgcca gactttgcca agtgtgttgc gtgggtcaaa taccatccca
    13861 gtcgctgctg ccgagagtcc ggacatcgcg atgcgccagc ccacaagccg gcatcccctg
    13921 tgtttgtcat cgacacccag agcggacaga cgcagcctct gccgatcgaa gacggaggct
    13981 ttggtgaata ccgtgcgctt gccgccagca gccggtattt cgtcgtcggt ggcgttgcca
    14041 acaaagccac tgtgttcgat cgcaaaacgg caaagcccgc tgccacactt tcggaagacg
    14101 gggcgtttta tgcattccgt ttttccgctg atgaaaagac gctggcggcg attcatctgg
    14161 gccgattcct gattgtgtat gacggcgaca ggttcacaaa acggacgaaa atagaagttc
    14221 tgccggatgg caagtggctg actgcgctgg cgatttctcc gtcgctgccg atcgttgctg
    14281 tcagcggttg ggatggcaat ctgcggctct attcgctggc gagcagcgat gtcggaaagc
    14341 tgctgtggag cggcccgctt ggacaggcct ctgccatcgc tttttcccca aagggaaacg
    14401 agctgctcgt cgccgtgtca aacccgaatc ggattgtccg tttggcggtg tcagtcgtcg
    14461 caggggatgg acagcgcgat cggtcgcttg gccatcgtta gcttgccggc accgagctgt
    14521 ccgcccgatc catcacccca gcacgacact ttgccagccc gtgtcagcgc acagaaaaag
    14581 gaatggccaa gctgcacact ggcgacatcg gacagaccga tgatcggctg cgctccggcg
    14641 gggggactgg gttcatcggg aagcttcatc gcacccggcg tttccccgta acagtgcacc
    14701 tgtccctgtt tcgatagcgt gcaggtatca cggggaccaa acgacacggc ggaaagctcg
    14761 ctgggaatcc gatcgacaag ctgcggagtc tgggtcgtaa gccgttggga gtcctgtgca
    14821 accactccca tgcagaccaa tttcccgtcg agcttctgcg cgcagagctg attttgggcg
    14881 gcaaacagcg ccacattttc ttttcccaag tcggatcgca caaacggacg cgaaaacgca
    14941 gtctgtatgt ctttgcccag cgctccgtat tcatttttgc caaagcagag cacctgtccg
    15001 tcttcgcgaa gtccacacgt aaaattgtgt ccagccgcga gctgccgaat cgggccgcca
    15061 aagccgctca ccagcgtcgg atcttccgtg ccaacaaggg ttccgtttcc gatctgtcct
    15121 gctttgtttt caccccagca aaagacactt ccatcgcgtt tttgtgcgca cgcatgctgg
    15181 gctcccatca ccacggaagt cacatcatga ccgagcacgc tgtgttcctt ggctcgccac
    15241 tccgttccaa cgcttccggg gggtgcagac agaaaactac ggccccaaca ccacagcgct
    15301 ccggcgtcgg tctgcgcgca ctgatcggcg cttccggtcc acaaacgggc gacgctgggc
    15361 ggcagtccgt aaatgcggta cggcagcgtc aagcggttca ccgataccga gtcatttcca
    15421 ccgcgaacac agaacacccc ctccgtggtg atcgtacaca gctcggacgt accaaaagaa
    15481 agcgaacgga ctcctttgcg caggcgggtg atggctctgg gaatcgagac ttcgtgtcgc
    15541 ttggtgtctg ggttaaagcc cggttcggtg tccccccagc agtacggggt tccggcccgg
    15601 ttgagcgcac acgcaacatg atgtgaggtc gcgatctggg ccacacatcc cgtcgacaga
    15661 ggcttgtcaa tgggactcag tccaaaacgc tggatcgctt gtccggagcg gagtttggga
    15721 actccttttt ctgaagaaaa gaagtctgcc gcaagctcga tctgttttgc tgaaagcagc
    15781 ttttccgcaa gcccaaggtc tgcgtcttcg gctgggaaat gctctgccag ctcggtcagt
    15841 ctgcgcgtga cggtcagggt ttccgatggc gcaagctcta gcggaatgtg cggatgcaaa
    15901 tccgcggtat caaccattcc tgaaaacgcc atccgatagg tctttccccc gtcggtacgc
    15961 accagaagcc gcgcgtagct tgcccagctt atcgaatgac aggtcgaaat cggaatcgac
    16021 ttgcggctgc tgcctgtgtt ttgcaaggtg atccggactt cgacatcgct tgggagcacg
    16081 ctgcgagacg gcgtcaccgt caggccaagt cgcagaccat cgacgacaga gccatagagt
    16141 tcggagtctt tggcttgtgc gggttcggcg aaaatacaaa ccaggacagc cagcggcagc
    16201 gtccgcgaaa gtctcatgtc gacatcataa cgccgaggct cgcgccgggg tcttggaacg
    16261 ccgggtattg tgtttgcgga ctggcgtagg gctagcgttc ccgacggcgg agcaatgcgg
    16321 ctgcaagcgt accaagaagc agcatgccga ccgcagcacc gctttccatg gttcccgaaa
    16381 acgaacaacc gggggaactg gcacctgttg cgacacaggc cagttgggtg gttccagcgt
    16441 cggtgacaca gcgcaggctg gcgcagcttc ctttggttcc gtttgcgaaa ctgcaggcat
    16501 ccccagccat tttgccgctg cacgctgtga tgtccgctga actggtgctg ccgcagctcg
    16561 gtgggggcga cacatcggcc cgcgccggaa tcgtcccaaa ccacgtcaga agaccgagga
    16621 ggacagctag acaaaggcgc atggtcgaat tcctttcgct ctgtttgtgg tcgcaccata
    16681 cctcgatcgg tttcgcgtgg cgatcgattc tgcgacgatt gattctgcgg cagcggcgtt
    16741 catccctacg ggaaagggga atggacgctg tttgcaaaga agggcgcgga tttttgtggc
    16801 gggacaggtt tcatggtacg ccgccgcgat gaacgtcgtc ccaacacgca gtttccacag
    16861 cggctggatc gcagtttctt tgggcgtgat tttttcgacc gcgattgcgg cgcagtcgtg
    16921 ggtcaaagtc cgcttgcatc gcgatcactc catcgaagtg acgggctctg ccaagcggcg
    16981 gattgtgtcc gatttgattg aatggaaagc cgacgtagcg acggaaggca aggaccgttt
    17041 tgccgcctac aagagcctgc acgatcatgt ggaacgcacg ctcgcgtatc tgaaagagca
    17101 aggcattgcg gaaaccgagc tgcgtgtgtc gtcggtgttc accgaagaaa tgagcgaaac
    17161 cgagtacatt ggcaaaggcg acgatcggat tgagcgcaag atccccaaag ggttccggac
    17221 ttcgcagacc atttcggtgt cgtcgaagga aatcgccaaa atcgaaaaag tgtcacgcga
    17281 agtgacacag cttatgtcga gcggcgtgcc ggtcacttcg gagtcaccga gctactacta
    17341 cacccagctt ggcgcgctca aaatcgagat gcttgccgag gctgccaaag actgtcggat
    17401 tcgggccgag cgcatcttgt catcgacggg cggggctcac ctcgggcggc tccggcgcgc
    17461 ggacatgggc gtcatcaaca tcaatccggc caactcgacc gaggtgtcga acgaaggcaa
    17521 cagcgacgta agctcgctgg aaaaagacat tttgaccatc gttcactgct cgtttgatct
    17581 gtcggaataa caactggccc caaatgtgca tcgtccggcg gaaaaaggag gaatccgatg
    17641 cgccatacga tgctttttgc ggtgtctgtg tttctgtttg cttgcggtcc caccaagggt
    17701 ccggtcacga cagtgggtgc cagcattttt acgtccgatg tcatcaccgt ggtggctgaa
    17761 aatcacggtg gtggctttgt tcccccaccg cctgctggat cggcctgtgc gctgggggcg
    17821 gccaagtaca cgctgacggt gggcagcaag tttttcgatt ggacgcgctg cgtttcgagc
    17881 ggaatgaatc cgtacaccat cgccaaaggc agtcgcacgc tgaccgatgc ggaatacaaa
    17941 aatctggtgc cgtatctcga aaatctgacg gtggtggcgg gggacggact ctgcggtgcg
    18001 gacaaaggga cgctcgatgt gaccatcacc acgccgagcg gccagcagac atacggcgac
    18061 agcttctatt cgtgttcgat aaaggacaag ccgctcgtga ggaccgatgc cctcgatcgg
    18121 ctgttttcac agctttacac gctggcgatg tagcggaatg tccgaaccta gcggcggcgg
    18181 gcgcgacggc gggcggcgag aagtccaatc agcggcagaa gggccaacac cgccgggacc
    18241 gaacctcgcg acgatgccgt tccacccacc gagcagccga ccgtgccttc cgctgtttgc
    18301 gcggacattg tgaccgtacc gacttcgggc ggcggcagag cggtggtaaa ggaacggacc
    18361 gcaccaaacg tgacaccacc cacaccgctc gcggcggcgc agtagtaata ggtcgtgccg
    18421 ggcgtaagtc cggtcagcgg ttccgacgtg cggtaggtgt cgtctcccgg ctgcggcgtc
    18481 gtgtcaaatc gcaggttgct cgtggccgac ggcacgacaa ggctgcgggc ttcgacgcgg
    18541 atgccgaagc tgtcgtcgca gaccgcgggg ttttgttcgc cgaggcggaa ccaagtgcgg
    18601 ctgccaccct ggtgctcgcg gactcggcct gccagggtcg ccgttgcgct gtcgacggcg
    18661 gctgggtttt cggtttcgac ttcgggggcg cgctcgccgg ggaccacttg gaccagctca
    18721 ccaagcgttg tgccatggct gttttgggcg atggcgcaga cgtaatacgt ggtgccgcgc
    18781 tggagtccgt acagcgtctc gctgatgacg acttctctgc tgccgctgcc gaccgactgc
    18841 tggttgtgtt ccggaacccg cttgccgaag ctgtcgttgc agctttcctg cggctcggcg
    18901 ctgtaacgga accaggccac tgtgtcgtcg ccgctcggga cgatggccgc gtgaacctgt
    18961 gccatctggg cggtcacgtc ggtcggggcc gacgtggtga ctttcggggc ggcagcgcgg
    19021 gtcgtaaact gcttgaccgc accgaagaag tggcctttgt ccgtcgagcc aatcgcgcag
    19081 tagtagtacg tcgtgccggc agacagaccg ctcatcgcct gagtgaacga caccgggatc
    19141 ttcgatgcac cgacggcagc gccgcccttt tgcggaccac ggatgccgaa gttgtcgttg
    19201 caagtgccag ggtcgcggct gcttagtcgg aaccagccgg tcatttcctc gctgctcggg
    19261 ttggcgctgc cgacgagaat cgccgtttgg gtgccaatcg aagtggcatc gagcgtgacg
    19321 attcgctgtg ccaaaaggtc aacgtccgaa ggcgtcggtg tgatgggcgg cgttgtgcct
    19381 cccggtgcgc tgggctttat cggcgtgcgc agcgtcgtcg gcgtcgtgaa cgacagcacc
    19441 gatccaaaca ccgttccgta tgcactgact gcgatggcgc agaagtaata cgtcgtgccg
    19501 ggcgacagac ccgagatcga ctcaccaaac ggcgtcgcgc tgctgccggc tccgagcgaa
    19561 gtgccaccgg acgtcggcgc acgggtgcca aagacgtcgt tgcaggtgcc ggggctcgac
    19621 gtgctgtaac ggaaccaacc aatcgtcgag ttgccattcg ggttggcagt gccgttaagc
    19681 tgcgcggtcg tcgcggtgac tggcgtcgcg gccagggtgt ttaccgtcgg tggcatcggg
    19741 gcgacaaacg aacgaatggt gccaaatgca gtgccgacgg cgttctgggc aatcgcgcag
    19801 tagtagtacg tggtgccggg ggtgagtccg gtcgcggcgt tgctgtacga cacaccgacg
    19861 acgcccgagc cgagcgcagt gccacccgtc gtcggaatcc gcgtgccgaa ggtgtcgttg
    19921 caagtgccgg ggctgctcgt cgcgagccgg aaccagccgg tggtcgccga gccgttcgga
    19981 ttggcggtgc cgttgagcgt cgtcgcaatg ccgctcggat agtcggtgct gaccgttggc
    20041 gggccggcgg gcgttgtgac cgacaagagc gcgcccatgc cggtgccgta agcgttttga
    20101 acaatcgcgc agtagtagta ggtcacgccg ggcgacaggc cggtgatgag ctggccaaac
    20161 ggcaccgtcg aaatgcccgc gcccaggctg atgcctccgc tcgtcggggt gcgcgtgcca
    20221 aaggtgtcgt tgcaggtgcc agggctcgac gtgctgtaac ggaaccagcc ggttgcggtg
    20281 gcgccgtttg gattgccgct gccgttgagc gtcgcggtcg tcggggtcac gctggtcgcg
    20341 gccaccgtcg ctgcggtcgc tggggccgaa gtcgtaaacg tcagcaccgt gccaaacgcc
    20401 gtgccctctg agctttgaac aatcgcgcag aagtagtacg tcgtcgccgg cgacagaccg
    20461 cttatcgact ggtagtacgg ctggtcatag atgtccgaac ccaggttgat gccaccgctc
    20521 gtcggtgcgc gtgaaccgaa cttgtcgtcg caggtgcccg gatcggttgc ggcatagcgg
    20581 aagtaaccga tggtggcgcc gcggtttgga ttgcccgatc cgttcagctg cgccgagctg
    20641 gtggtgatgg tcgttgcggg cgtcgtcagt gcggtcggtg cggtcggcgt gataaacgtc
    20701 tggaccgttc cccaggccac gccctgcgag ttctgggcaa tcgcgcagaa gtagtacgtc
    20761 gtcgccgggg ccaggccggt aatcgcctgc gagaaggtca cattggatgt gccactgccg
    20821 agcggagagc cgcccatcgc cggagcgcgg gtgccaaagg tgtcgttgca agtgccgggg
    20881 ctcgcggtgc tgtagcggaa ccagccggtg gtcgcatcgc cgccgggatt ggcatagccg
    20941 tagagcacgc cggtggtgcg cgtcaggttg ctggcgccgc tggtcgtcac tgctggggcc
    21001 tgcaccgggg tcgtaaacga caccaccgcg ccgtagccga tgccgtaact gttctgggct
    21061 atcgcacagt agtagtactt ggtgccgggc agcaggctgc ctatcgactg cgcaaacgac
    21121 gtcggactaa agccagcacc gaggtttgtg ccgccgctcg aaggaatgcg gctgccgaag
    21181 ctgtcattgc aagcgccggg gttcgtcgcg ctgtagcgga accagccggt ggtgctgccg
    21241 ccgttgggcg tgccggtgcc gctcagcatc gcggacgtcg aagtcacact cgcgggcatg
    21301 ccggtgacgg ccactgcggg cgatgcagtc gtaaaggaca tcactgcgcc aaacgccgtg
    21361 ccctcgctgc tttgaacgat ggcgcagtag tagtacgtcg tcgctggcga caggccagac
    21421 accgtctggt aatacggctg gtcgtagatg tccgaaccca ggtttatgcc acccgaaagc
    21481 ggtgcgcgcg aaccgaactt gtcatcgcag gttcctgggt tagtggtcgc ataacggaac
    21541 cagccggtgg tgtaggcgcg gtttggattg cccgatccgt tgagctgcgc cgaggtgtcg
    21601 taaatggtgc ccgcagccgt ggtcaatgcc gacggtgccg acggcgtgat gaacgactgg
    21661 atcgatccaa agacgatgcc ctgcgcgttt tgggcaatcg cgcagtagta gtacgtggtt
    21721 gccgccgaca gaccgcttat cgcttggcta aacgaaacgt tggatgtgcc agcgcccatc
    21781 gccgatccgc ccgtcgccgg agcgcgggtg ccgaagctgt cgttgcaggt gccggggctc
    21841 gcggtgctgt aacggaacca gccgaccgtc gccgatccgc cggggttgcc gtagccgctg
    21901 agcagcgcgt cggtgcgggt caggttgctg gcgccgctcg tgctgaccgt cggggtggtc
    21961 gctggcgtca caaacgacac gatggcgcca aagcttgtgc cgtagctgtt tgtgacgatg
    22021 gcgcagtagt agtacgtcgt gccggggctc aggctgccga tgccctgctg gaagttggtc
    22081 gcgctggttc ccgcacccag gtttgtgccg ccgctcgatg gagcgcgggt gccgaagctg
    22141 tcactgcagc tgccagggtt ggtcgcgctg taacggaacc agccggtcga cgtgccgcca
    22201 ttgggcgtgc cggaaccatt gaggatcgca ctggtcgacg taatgctggt tgccgggttg
    22261 gtggtcgccg ttggcgctgt tgccgtcgtg aaggacagga gcgcgccaaa tgccgtgccc
    22321 tcgctgcttt gcacgatggc gcagtagtag tacgtcgtcg ctggagacag gcccgatagc
    22381 gtctgattgt acggctggtc gtagatgtcc gaacccaggt ttgtgccgcc cgtgaccggt
    22441 gcgcgcgagc cgaacttgtc gtcgcaagcg ccggggttcg tcgtcgcata gcggaaccag
    22501 ccggtggtgt aagcccggtt cgggttgccc gatccattga gcagtaccgt ggtgtcggtg
    22561 atgttgctcg ctgccgcggt cagggccgtc ggtgctgacg gtgtgatgaa cgtctgcacc
    22621 gatcccatcg ccatgccaag cgagttttgc gcaagtgcgc agtagtaata ggtcgtcgcc
    22681 ggggccagac ccgagatcgt ctgggtaaac gaaacattgg tgttgcccga accaagcgcc
    22741 gatccgccca tcgccggagc gcgggtgcca aagctgtcgt tgcaggtgcc ggggctcgcg
    22801 gtgctgtaac ggaaccagcc ggtcgtcgcg tcgccacccg ggtttgcgta accgctcagg
    22861 accgcgccgg tgcgggtcag gctcgatgca ccactggtgt ttaccgtcgg agccatcgtc
    22921 agcggcgtaa actggacgac ggcgccataa ccagttccat aggtgttgct gacgatcgcg
    22981 cagtagtagt acgtcgcgcc tggcgacagg ccgctgatcg tctgcgcaaa cggggagctg
    23041 gtggtgccgg cgcccaggtt tgtgccgccg ctcgacggag cacggttgcc aaagctgtca
    23101 ttgcaggtgc caggattggt ggtgctgtaa cggaaccagc ccgaagccgt cgcgccgttg
    23161 ggtacgccag atccattgag tgtcgcggtg gttgatgtca ctgccgacgc tgcattggtg
    23221 gtggccgacg cgatgcccga tgtcgtaaat gacatcacgg tgccaaacgc cgtgccctcg
    23281 ctgctctgga cgatggcgca gtagtagtac gtggtgccgc ccgacaggcc cgaaatgctc
    23341 tgcgcaaacg tctggtcata gatgtcgttg cccagagcgg tgccgccggt gagcggcgag
    23401 cgcgagccga acttgtcgtc gcacgtaccg gggttggcga tgctgtagcg gaaccagccg
    23461 gtggctgaag cgcggtttgg gttgcccgat ccgttcagct gcgccgaact ggtggttagg
    23521 ttggtcgccg atgccgtcgc tgcggttgga gccgacgggg tgatgaagga ctggaccgaa
    23581 ccgacgacct ggccgcccga gttctggaca atcgcgcaga agtaatacgt cgttgctggc
    23641 gcgagtccgg tgatcgcctg cgtgaaggtg acgttcgatg ttccgctgcc gagtgccgta
    23701 ccacccgtcg ccggagcgcg ggtgccaaag ctgtcattgc aggtgccggg gctcgcggtg
    23761 ctgtaacgga accagccggt ggtcgcgtca ccgccggggt ttccgtatcc ggacagcgtc
    23821 gccgacgtgc ggcttagatt gtacgcgccg gatgtcgtca ccgtcggtgc gttggccggc
    23881 gttgcgaacg tctgcaccgt gccataggcg ttgccgtagg tgttgctgac gatcgcgcag
    23941 tagtaatagg tcgtgccggg ggtcaggccg ctcagcgaat acgtaaacgg catgctcgcc
    24001 gcgcccgatc cgaggtttgt gccgccgctc gacggagtgc gggtaccaaa gctgtcgttg
    24061 cagctaccag ggttgctggc gctgtagcgg aaccagccgc tggccgtcgc cgcattcgga
    24121 ttgccggctc cgtttagcgt cgccgtcgtc gccgaaatgc cggtagccgg gttggtcgtc
    24181 gccacgggca ccgtcgcagt ggtaaacgtc aggatggttc cataggccgt gccttcgctg
    24241 ctctggacga tcgcgcagta gtagtacgtc gtcgctggcg acagacccga aatcgactgg
    24301 gaataggact ggtcgtaaat gtcgctgccc agcgcggtgc cacccgacag cggtgcgcgc
    24361 gagccaaact tgtcgtcgca ggtgcccgga tcggtcagcg cgtagcggaa ccagccgctc
    24421 gtcgccgctc ggttgggatt gccaaggcca ttcagttgcg ccgtcgtgtt ggtgatgtac
    24481 gtcgcgaata ccgtggtcgc cgtcggggcc gacggagtga taaaggtctg caccgaaccc
    24541 cacgacaggc cctgcgagtt tttggcgagc gcgcaatagt aataggtcac gcccggcaga
    24601 aggccgctca ccgtctggct gaacgacgca ttgctggtgc ccgagccaag cgaggttccg
    24661 cccgtagccg gtgtgcgcgt cccccacgta tcgttgcagg tgccggggct ggccggtccg
    24721 taccggaacc acgcggtcgt cgcgtcgcct cctggattgg catagccgtt aagctgcgcg
    24781 cccgtgcggc ttaggttgga tgcgctgttg gtgctgaccg tcggggccat cgatggcgtg
    24841 gtgaacgact gcaccgtccc gacgctggtg ccaagcgagt tctgtgcgat ggcgcagaag
    24901 tagtacgtgg tgccggggct taggctggtc agcgactgcg cgtaggcata cggggtgatg
    24961 ccggtgccga ggttttgccc gccactcgat gggatgcggc tgccgaagct gtcgtcgcaa
    25021 gtgaccgggt ttgtcgtgct gtagcggaac cagccggtcg tcgacagcgt gaacggattg
    25081 gcggtgccat tgatcgtggc gctgttgctg gtgatgctgg tggcggcgct ggttgtgacc
    25141 aggggctggg ccggaatcgt aaacgacagc accgagccat acgccgtccc cgacgcattg
    25201 gtgacaatcg cgcagaagta ataggtggtg ccgggggtaa gtcccgtcgt cgtgatcgag
    25261 tacggaaccg tcgcggagcc gttgcccaga tcggtgccgc tcgtcgctgg tacgcgcgta
    25321 ccaaaggtgt cgttgcaggt gccggggttg gtggcgctat agcggaagcg acctgtcgtc
    25381 gcctcaccgc cgggattgcc cgacccattg agcaccgcag aggagctggt gatgccgctg
    25441 gctgccaccg tcacaacggt cggagctgcg gcaaagcctt tggccgaaaa gccaaacaaa
    25501 accacgagca aagctgctcg caaaatagaa atcagcacga aaatcccccc aggaaaaaca
    25561 acctcgtaca ttaagcaaaa tcagtgtgag agtcgattgg aaaatgtagt ctgagtggtg
    25621 tttccgacgg aaatgatagg gctgaatgga agatcgtttt gcttttgatg ataggtattg
    25681 gcgcttgatt tgatatcgaa atatcagtgc tttgggtgat ttgtgcgaag tctgatttgt
    25741 ggacttttct gcgcatttat ttgggcagaa aacaggtcga tcattgcgcc aaatatgcaa
    25801 agtcaaagca caaatgggcc tttggcgggt cggctgggcg aatcgatcgg gctacggcag
    25861 cggggtttcc atcgcggcgc ggatctgtga agcccgcgct tccaccacct gacgcagcgt
    25921 gacaaagcgt ggttcgtcgc gcagaagctc aagcagcggg cactggtcga tccacatgac
    25981 gtcgcacagt ccatcttcga cacaatcggc cagcgaaaga agggccgctt cgcgcttctg
    26041 ttcaaaacag agcagctcgg tccggccttg gtgcagcatg acccgccgcc gagggttggc
    26101 gcttgaaccg gcttcgaata gttggcgaaa ttcgtcatcg cttagcgagg ccgacccatc
    26161 ttcactggcg gcgacgcgca gcagtcgttt gccacgctgg cagcccaccg attcatcccc
    26221 tggcatcgtc agtttctgct tggcccacgc cgggtctttt cgccacagcg ccatgcgtcc
    26281 ggtgatcatc caccacagcg ggactgcgcg cggatcattt agttcttgct cgagcagcga
    26341 atcgacctgc ttccacgccc cggtcatcgc aaacagacgg gccatttcca atcgcggcct
    26401 ggtgctcagc ggatcgagcc gacttgcggt ttccaggtgt gcgacagctt ccgacagcgg
    26461 accacactcg cacaggattc gcccgagcaa gtcgtgacaa tcggcaagct gaggtgcaat
    26521 cccaagtcct ttgcgcagcg cccggaccga gcccggcaga tcgccggttc cataacaggc
    26581 cgcaccaagc gccaaataag gtggtccctg gtttttcgca acttcgacgg cccgctgggc
    26641 agcgcgaatc gccttgtgct cgtcttccga atgtccccaa aaccacgccc gcgcacaggc
    26701 tttggcgtag ccgaccaaaa gaagtgggct gtttggtttt tgggcgaggg cttgttccaa
    26761 aagctcgacg ctgtgatgga aaaacgacga atcgttgccg gtgtaaagct gccgtgcttt
    26821 gaggtacaga ttcagcgttt cgggatcgag aacgtcgtca ctttgcggcg cggtaacttc
    26881 gctggtgagc gcgacggcga tctcctgcgc ggttttttgg cacatcgaaa gtgcttcacc
    26941 aaacggcagc tcccagcgac ggcaaaaaag ctggaaacca ctgtcgaccg cgagcagacg
    27001 cagcgtcact gaaagcgtgg ttccttgcag cgaaaacgca ccacacaaaa atgcatgc
//
LOCUS       DAORGN010000050        25820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_742_length_25820_cov_6.260741, whole genome shotgun sequence.
ACCESSION   DAORGN010000050 DAORGN010000000
VERSION     DAORGN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 25820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25820
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_25820_cov_6.260741"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PKL17_07800"
     CDS             <1..1825
                     /locus_tag="PKL17_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44670.1"
                     /translation="KVYTENVEVKAAETRPVWVIMASDVKTGALIVSADVPADVVVDG
                     QPKGQAPVVVDNLTEGEHVVEVRRNEPGSPPWRQNVRVVGNQQTKVHANTTPPPPAAG
                     TLMVVTSVGDADVQIDGVSKGAPGQPISLSPGQHSITVSAKGHKAVTKVVEIEAGKPR
                     VERIEMEGDAVTRKVGLIRIVMANPIDGAQYYINGRRYDETAALSDQGVEIASGKVIV
                     VAKKDGFGQDSKEVTLAAGSTETVTLNLRNVGKVYIASEPKGAYVTLDRVLIGQTPLT
                     RENIGQGPHTVEFQMPGFEQKVEQVNVRSGETEQVSVMLVKIKEKDEDPKEVRRGLSS
                     FSAVTNKFGSFTADIGSGYPWIGNVRLTVGVWKTKHSALGEIGLDIGTEFRSNFYSDH
                     EAAGTIRFQMMRKGPFALGLHMLVGGGGGPRGRNSFSWELGVPVTILAGSMVKLTGKA
                     YLQVYSDRYCPSEDAIREMYKNDRNKFVRMGNPADTSDGGGAEHTGDRCVGRSYDPKI
                     SFPSPIYAVEQYNGTRKYDASLPDFQVDGTGVLERFVSVRGMLQAAVELALTSNVSFY
                     GILEGAPFQKERQLFTDKFNRLFPIHDTPIYGRLGLTAKFF"
     gene            complement(1903..2862)
                     /gene="msrP"
                     /locus_tag="PKL17_07805"
     CDS             complement(1903..2862)
                     /gene="msrP"
                     /locus_tag="PKL17_07805"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNK44671.1"
                     /translation="MLIKKPDDILGREITPRADFLSRRRFLIGTGMAGASLGAGVYLF
                     GQTKTSKAESAQKLAATKRADFSVSEAPNSYKDVTTYNNFYEFGLGKADPAENAHTLK
                     TRPWTVRIEGEVQKPRTVGIEELLKLAPLEERIYRFRCVEAWSMVVPWIGYSLSELVK
                     LCQPTSKAKYVQFVTLKDTAQMPGQKSWALDWPYVEALRMDEAMHPLSLLCLGVYGEV
                     LPNQNGAPVRLIVPWKYGFKSCKSVVAIRFTEEQPVTTWNKQAPGEYGFYANVNPEVD
                     HPRWTQASERRIGELRRRKTLPFNGYADQVAKLYSGMDLKRFF"
     gene            2995..4773
                     /gene="sppA"
                     /locus_tag="PKL17_07810"
     CDS             2995..4773
                     /gene="sppA"
                     /locus_tag="PKL17_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNK44672.1"
                     /translation="MPAAIRRFLTFIGGITVLFVILGIVLVSTATRKKAVPQQVILEL
                     DLEREVVEYLPDDPIAQAAGGERIVLHNLTMTLERAERDQRVVGLVARLGAAKVPVAT
                     LQELRDAIIRFRQKGKFAIAFSETFGEFQNGLGAYYLATAFDEIWMQPSGDIGLAGLY
                     LESPFLRGVLDKLGVKPRLDQRKEYKNAMNMFTERAMTPAHREAMTKIGESILGQIVR
                     AMGESRKLSADEVRALIDKGPLLGAEAVTAKLVDKLGYHDEFYAAAKQRSSGAELLYL
                     HKYAERTEPLHKSGPRIALIYGVGGVSRGKSSVDPLSGDATLGSDTVAAGFRAAILDP
                     EVKAILFRVNSPGGSYVASDVIWQEVGRARRAGKKVVVSMGAVAGSGGYFVAMAADKI
                     VAEPATITGSIGVLGGKLVPAELWEKIGLSFDAVQLGKNAAFFSMNRDYSPEEWARLQ
                     AWLDRVYDDFTSKVATGRKLDKQKVLEIAKGRIWSGEDAKGLGLVDELGGLHTALALC
                     KQLAGIADGQDVELKTYPQERGPLKAIVAKLTHKDPDSSEKEGAQVRMQSDALQSALR
                     VVQMAKQLGLVGSQDVLQMPAVELGF"
     gene            complement(4789..5427)
                     /locus_tag="PKL17_07815"
     CDS             complement(4789..5427)
                     /locus_tag="PKL17_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNK44673.1"
                     /translation="MPIALDVNVLPRGQASRTRKLYNAFFDTWLEKDPERRRISLNLA
                     DDYRSLPVFDEWDITAKFEMLYGEGKLDERGAERWAALTQLTDQLHSADMVVITAPMW
                     NFSIPWHLKRWIDCVVQGRLTFEYLDGQFRGLLGGRPAVLLTSRDGIYTPGTELAALD
                     FQVPYLRTILGLIGLNPIHEVIAEPMMLAGPDAGVAALQVAVDKARALAESL"
     gene            complement(5445..6062)
                     /locus_tag="PKL17_07820"
     CDS             complement(5445..6062)
                     /locus_tag="PKL17_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44674.1"
                     /translation="MDRSNVTLAQLARGIAVRFVQPAVIDAALTALGTRADIVLSELT
                     TERRSTLINHFVRLLRNTGIQTATRVEHAMLEAASCRTAEKRVISLKDAISLIAVRNL
                     IHQMATAVGIGWNDSMKLQSALSDISRFIIEKGGGKVEIKDTGSALVFLIDSLADLTA
                     FSPPQIPSPSWLASTLNLAQGFRTTLSGGGTHFEFWYARPHSMVA"
     gene            complement(6328..6939)
                     /locus_tag="PKL17_07825"
     CDS             complement(6328..6939)
                     /locus_tag="PKL17_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNK44675.1"
                     /translation="MQKPKLTYFDAPVSRGEECRLALHIAGVDFEDVRIKPADWPALK
                     PATPFGGLPTYEEPGRPLLAQTNAILVLIGRRHGLHPDDPFEAARHEAVMHHVEDLRA
                     HVGPTIRIADPEEKRRVREGLVASYLPVWAEQTERQLGDGPFFAKDKLNVVDLKLYLA
                     VRWFAGGKVDHIPATIFAAYPKLNRVHDAVRDHASVRAWYAKS"
     gene            complement(7097..8614)
                     /locus_tag="PKL17_07830"
     CDS             complement(7097..8614)
                     /locus_tag="PKL17_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK44676.1"
                     /translation="MQQPKTTRLAVAFVGLCSLVQSSGCTDLWKAQLVANPKNCAIAP
                     AICGDLEECDYETERCVPLMPPACPQISFDAIHPQFGSRGGGTPITITGSNFQGSMRV
                     EIDGTPLDNIQIDSPTQLRGTLGATINSCGKSPVTVISSCFERVSKESAFSYSLDPLT
                     FDSQPQILPSSPTSSVQQIVIDDFNADGDPDIIGIESASVRLFLSDGNGSFTTTTPVS
                     LAGTLFQAAIGDANGDKAIDLMVSDASNPRLWLLLNSGKGVLSTQSIPMPEPLRGIAS
                     RDLNADGRDDVVAVGISGTMYQLLGATTGFLSPDVVANGLPGQSQLVTLADLTTDGQP
                     ELVVAGGNSQFVEVWQKTGAKTFARLSQNKVPAAAYSVAAGDINGDGLPDVVAGLVGQ
                     RNVAVLTAQGGGLFQSAVTVPSGVIPRHVQLVDINCDGKLDLLVSSATENMLGMLLGN
                     ASGTLSAGSTIDLSAQQPGTAQSLLARDVNFDGTVDLVVGSSQSPAYLLIANTSP"
     gene            complement(8630..10186)
                     /locus_tag="PKL17_07835"
     CDS             complement(8630..10186)
                     /locus_tag="PKL17_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK44677.1"
                     /translation="MLIRSRIDHIKSKSLLLAGALSVSVWASCDSLWKDFTISTAPQD
                     PCKNAMCAAYQVCNSTSGACESTPLRFDGITPTTGSLYGGTQVTIKGAGLASDTAAKF
                     GGLLIDGPVFDKTGTVLSGIAPKPTQSLRCRMPVDVELSNPRGDKIAKTAAFTYLFDP
                     FTSNSFLPLGAVSSPVNKILVGNLDQDTNMWPDLLFRTETGVLLARNIGTTIAMPDQV
                     YSPAPSHLVIAQSGPPTTGMSSAPYIVLGFAANHQVKLLQYYNGLVTENSFDSIYPLL
                     AMTTADLDRDGLDEVIYAASKPGTQSSELYVLHRANLGSGFGRVGPFFVPNIVAGIAG
                     VDLGGDPNLELVLTSSSSSDLTIFGWDGTKTLPVSIPLAVPGIMFGQVISADLDGDKK
                     NDLAFLSTQANTSATLVVALNRPTRWDSLTFPIPDLIGTPDTELSTYDMNCDGIQDIL
                     INQFGPNPKTLFFYSGGNGTFTPKAPQSTQPNGALAVADINGDGFPDLLVGRNVLNSN
                     SMLSIAPGNK"
     gene            complement(10224..11768)
                     /locus_tag="PKL17_07840"
     CDS             complement(10224..11768)
                     /locus_tag="PKL17_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK44678.1"
                     /translation="MIHIQRPTFTQIATLSLLSALSQSGCSSLWNGFTTGDPSSCVNT
                     PTLCSQAQVCNRDTGMCEASTVSDYFSYTGINPSMGKTGGGDAVDLRGTGFTTDTVVK
                     IAGQPLVQPMVQGNGTVIHGLTPVSPGRCGPVNVEVTRTGFPAALHPAEFLYKFEPYH
                     NAPPFALANLNEEVLQIAQAEFTGDRFPDLIVSSPKTLYFVSNNAGAAGPAENTGLLF
                     SKPIRFSVGAFQLPGRTAAAIVDPNTNALLFVNTVSSFTPAYNNGTVPKDVATINLDA
                     VGTDEVVIYGSNTSSTNYYLTPARESATGNVSAGSTVNLGGSPLPAMAVLDITGDGFP
                     DVVLGGANSTSLVYAKSTGSFGLTYAGAPAITLPVGVSSLVAADWDRDGKTDLAALGT
                     DGRVFFLKNQGNGSLIKIDEQPTTIKSAGGVTEPGPPMQAYDVNCDGLLDLILGPSAS
                     VSPINNITIFLNDGQGHYPPKQTLQNTGGSFLFTDLNQDTLPDFVMRDPVIQRTGLNV
                     AMGSIP"
     gene            complement(11784..12329)
                     /locus_tag="PKL17_07845"
     CDS             complement(11784..12329)
                     /locus_tag="PKL17_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44679.1"
                     /translation="MRYFVLLLAASLCVPNVGAAQTAAHAPLSAPRIELPTQIPAVEP
                     SPAEPEPDPSEYEPPPAAPKVLSVLPRSVGPSERKYRWPLWRILTGSALVVGGGVMFG
                     FGMSAFAFDGICVPNPPPGVECRRYYDTKTQGIALTTGGVLTAVGGLLLISIPSKVSR
                     PKPAAQLLLPGLSISLSKQTD"
     gene            12408..12914
                     /gene="sixA"
                     /locus_tag="PKL17_07850"
     CDS             12408..12914
                     /gene="sixA"
                     /locus_tag="PKL17_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNK44680.1"
                     /translation="MRKLTRPLRIRHADGVKIYLIRHGHAVDAELHLADGERYLSKKG
                     RRTTREVGRALRKASVSVDAILTSPLVRAVQTAELVAEQLGFEGVIEVLPSLMPIGPI
                     ETSAEVIREQSRTVAVVGHEPSISALAGLLTGQGHFPPLRKAQVVCIHGDRAVWTIDP
                     DRLDPVSL"
     gene            complement(12970..13641)
                     /locus_tag="PKL17_07855"
     CDS             complement(12970..13641)
                     /locus_tag="PKL17_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNK44681.1"
                     /translation="MKAEKKPEFMPIPKQVLPTLRKRDRKMAWLIDQVGPFEMGPPRQ
                     RTHLEALCRSIVFQQLSGKAASTIFGRFRALWPTGEFPTAPQIMQKPDESLRGCGLSG
                     QKVSYLRDLCQKLIDDPHFLSDVDALDDEAVIDRLTSVRGLGRWTCEMFLMFHLGRLD
                     VWPVGDLGVRKAVQRLHGLDDLPPPKQMQELGQPYAPYRSVASWYLWRLLELPEETVA
                     LLPAR"
     gene            13749..14999
                     /gene="ribD"
                     /locus_tag="PKL17_07860"
     CDS             13749..14999
                     /gene="ribD"
                     /locus_tag="PKL17_07860"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNK44682.1"
                     /translation="MTVSTPHAGAAAFTERLLPGERSHDAAMMRLAIAAAETARGATS
                     PNPLVGAVIVDESVSPPQVLATGFHAQAGRAHAEVDALQKLAQTGASATKKTLYVTLE
                     PCNHVGRTGKCTDAILAAGLARVVIATADPNPHVAGGGAGRLREAGLDVLVGVCEPQC
                     QWQNRGFLRWLKSGRPQLLLKAAISLDGRIAPQAPPDTVIGPRWLTGGPARQKAHQLR
                     AASDAILVGAGTVLADDPQLTVRLFDREVVRQPLRVILDGALRVPTTAKALGPGSLLV
                     TCESSFQAQPDRVAALQSIGTEVIPLPLPSSPDVANRRGAATDVDLHAVLRLLGQRQV
                     LYALCEGGGVLHGALLESGLCDEAALFVAPLFLGDGGVPLLRHLAYPDVTAAAWIETP
                     QYSQLGDDLFVTGALRRGGFAPSA"
     gene            15019..15681
                     /locus_tag="PKL17_07865"
     CDS             15019..15681
                     /locus_tag="PKL17_07865"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNK44683.1"
                     /translation="MFTGLVEDLGRIIEVSPLPRGSGQRIGLSSPLLSQPLPLGASLA
                     VDGVCLTVVAASPGLASVEVGPETRARTTLSDLAPGRVVHLERALQVSDRLGGHIVTG
                     HVDGLGHVSKSVRRGDSWDVEVNTPQELLRYIVEKGAICIDGVSLTVNSVSARGFSVS
                     LVPHTQAHTHLAQKSVGGAVNLEVDIIAKHVEKLIAAYLPTQPSGLSLTKLKEFGFAR
                     GD"
     gene            15665..16819
                     /gene="ribB"
                     /locus_tag="PKL17_07870"
     CDS             15665..16819
                     /gene="ribB"
                     /locus_tag="PKL17_07870"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNK44684.1"
                     /translation="MHAGTSTTERAARVKRALADILAGKMVILVDDEDRENEGDLVMA
                     ADKVTPEAINFMARFGRGLICLTLTEEWADHLQLPLQSSRAGGPPLGTAFTVSIEARH
                     GVTSGISAADRAHTILTAVAKNAKPEDLVTPGHIFPLRAKRGGTLVRTGHTEGSVDLL
                     RLSGLTPAAVICEIMNDDGTMARMPDLLRFAKQHDLLVLSIADIIEHRLQTESLVEKV
                     ETFELTPTINGQKAKRFSGALFRTAIEPTEYLALWIGDLSGDDPALVRVQTASLPGDV
                     FGALGCDSGAQLQAALRIIDSAGRGVLLYVFPTGRFNVRDDLRAHLLHRTDVIPSGNK
                     LRDFGLGAQVLRELGVQSMRLLTNNPKKIAGLTGHGLKLVESIPLLPQLP"
     gene            16859..17353
                     /gene="ribE"
                     /locus_tag="PKL17_07875"
     CDS             16859..17353
                     /gene="ribE"
                     /locus_tag="PKL17_07875"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNK44685.1"
                     /translation="MSSEDQTKVIEISGSLSAAQAKIAIVASRFNSFIVERLIEGAVD
                     VLRRVGGAPESISIIRCPGAFELPAVTRRCVESGKFDAVICLGAVIRGATPHFDYVAA
                     ESSKGIASVAAQATIPVVFGVLTCDTIEQAVERAGTKAGNKGADAAMTALEMISLFRK
                     LGHG"
     gene            17346..17855
                     /gene="nusB"
                     /locus_tag="PKL17_07880"
     CDS             17346..17855
                     /gene="nusB"
                     /locus_tag="PKL17_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNK44686.1"
                     /translation="MAKASGNRRRARESAMQILCVLDRQPELLPGQATALYFAHLLGD
                     DGESSTSIDERTLPSDYRLFAETLVSGVRKAQSDIDALLSRSSKNWRLSRMAVPDRNI
                     LRLACFELMFCPEVPARAVLNEAIEIARRFGTSDSSSFVNGVLDRVVVELGRRQDTEH
                     ATESESTPA"
     gene            17881..19260
                     /locus_tag="PKL17_07885"
     CDS             17881..19260
                     /locus_tag="PKL17_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44687.1"
                     /translation="MNKAFLLLFLACIACACEPLPYSPHRATESPLPKNAFVHLVVVS
                     SPSPSVEVSISNQVRAVVPRRGISPRMELSSGNYPLLITGGGSTLCESSLQLLPGSET
                     LLIASAQNTDDKKCPSLVLSAYPLGQRDPKLARVRLLHSALDAPMLELVGPSGEEIVD
                     GIAAGAVSAYGGLASPVAAGSTLRLRSPLDKQPLFDVMTDGLSLGSASTWLGVGEIGP
                     LASQNAFGLLSFDEESGALRELSAVPSAGAPDGEVLLFHASKEVGSLVAKVAGKPLFS
                     ALSYPHASPLATLASGLRTLSLEDPSGTVWTGPLRLWPGRGWLLFLYGTRSAPRLVAL
                     PRPALAPQTVWRVLNLVDGLDSVDLLDGDDAMVSQLGFGAATAPTLVDALRPGTIRLR
                     DRGAGNRSWDIVLTAGAATASLDQVVTLVLLGSASSPRSVSAQLVVESRAGLMTAPPV
                     LALPTSPSL"
     gene            19416..22247
                     /locus_tag="PKL17_07890"
     CDS             19416..22247
                     /locus_tag="PKL17_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44688.1"
                     /translation="MPIQQAQQLGRYYLLDRVAFGGMAEIFRAKTRDAQGKELLVAVK
                     RVLAHLCEDDEFIQMLIDEARLTALLKHPNIARVYEFSKVGSEYFIAMEYVEGKDMRA
                     LLERARQNQEWLAEDLIAYIGMQVALGLHFAYEQKDRDGTPLHIVHRDVSPSNVLLSY
                     GGDVKLCDFGIAKAEGARQQTRTGVIKGKVKYMSPEQAMGRTLNNRSDLFSLGTLLYE
                     MLALQAPFTAQTEIELLFAVRDARKRPLRELRPNIHPELERIIDKAMERQEKKRWENG
                     QEFAEALGTFLQYHYPNTNMASLSRCMATMFAREIDKERAAMAEFVIDTSRFDENVGE
                     NLLADVLGPDAEYTQFTAAFGRQDIADPLPSTPTGPNPNQMRVLASVSMPGTVDFDML
                     QTTEKPRYEAVGMMPPVSPAPGEPGWMPSAQTMPRGARPSQAMPPVPMGPRPSQAVPQ
                     LPILGDTQPNGESGFETVIIQREPVLEKRPGGPIAPPIIDEQFHAEETRVLSRDQVDF
                     LKLIPQKSSPAPQPVQSAQKPFEFHEQATQILDARSLPAELQQLLARKSAELAAAVKQ
                     APVTPPPSAPVPSGKARAPSIQLQPLDSDFHDAATEYLDWSPIEPSAIEEMSLDPSSA
                     NFGAFRQGTGAHAPVALPRPATSAQPQIAVTPPAAALPPRPPASSAAPIPPPRSATGA
                     QPPVGLPMPPLSPSAKSPPPPPPAAAKIAPPPPRSATGAQVPVGLPLPPLSASMKSPV
                     PPPPPSKSPAPAPAPPLAPPALAPSLRAATPGARPTGLQPQIGRTQTAPPLAKAPPAP
                     EPKPAPAPGRVAAIQPPGEDEWEALPHLDSTMLARAGSVPVVLPRSALPARPTGRVDA
                     IPPAGQKFDPLEHTMQTDDEHMSPSVGDSPLDDDQPTPVRGTSLSDMGLGTESEFAED
                     TDKGDGPKGFNKHPDPSLTFEGDDLEPVD"
     gene            complement(22254..22991)
                     /locus_tag="PKL17_07895"
     CDS             complement(22254..22991)
                     /locus_tag="PKL17_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HNK44689.1"
                     /translation="MADSPSLPSPSGNDCPSNGDSRPKWPIRLWMSFLRAHRRFGFAV
                     AVSLLTLLLVPARLGFVVRIVSAWDSGSLAMLALTWWIMIRSGPQKTRRRAASEDPGR
                     TLIWLIVLASCGFSLLAATALINQAEHLANAPLRLLLVGLCLLAVASSWLLTHTAWAL
                     RYAHLYYRQDGAGEGGLSFPGEEPPDDLDFAYFAFTLGMCFQTSDVAVLSRTIRRYVL
                     LHALVSFVYNTSIIALSINLASGLFGG"
     gene            complement(23073..23771)
                     /locus_tag="PKL17_07900"
     CDS             complement(23073..23771)
                     /locus_tag="PKL17_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44690.1"
                     /translation="MSRPNYRVILSFDSERKTFTARVPELPHCTAEGMTRAEAILHVE
                     EELAALYANLAAQGKSPPPSIDEQAQSGEFTVKVSRGLHRELLWQAASEGVELSQLAG
                     ELLSSALESRRNLRPRRGQEGNQAHPDHRPPRHDGGGDRRRGGPQGGRFGDMMEDRAH
                     FIEYVRNLEHSDNRRGGGGGGGPRGERHDGGRRGGGGPRGGHGPNRDRRPSGDRPHSS
                     GDQGAPSGNSERGS"
     gene            complement(23869..24708)
                     /locus_tag="PKL17_07905"
     CDS             complement(23869..24708)
                     /locus_tag="PKL17_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44691.1"
                     /translation="MKNVYLVVEGVHDAAFIGRLLTKHAKLAQIKEQTKLPPGWQTFV
                     DNFKWPTRNRQGVLDISRLAVPAPLFFGHPQHDLMVAVSNAEGIANLAARLESDLEQI
                     LLGKLVLDGVGVVLDSDEEDAQARLSKLLAELRARDVQIPIPDKPELGVVKHGKPRFG
                     VFAMPSPTRKGTLEDLLLDCAQAVYPKLLTQAQGLVDRRSEFLQELEADETKLLENKP
                     AGRKKATVGVMGAFLKPGKAIQNSIHDHRWVSKETLSLPQLAPLVTFLDALLDAAQGC
                     RAV"
     gene            complement(24705..25766)
                     /locus_tag="PKL17_07910"
     CDS             complement(24705..25766)
                     /locus_tag="PKL17_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK44692.1"
                     /translation="MLLHDLHIGGYRGLRDLHLEGLGRVNVLIGQNNSGKSSVLEAAA
                     LLARPFDDAQWTRVIQSRDSFLSDTPPHALWELFPCAEPLLFGGTRPKAEAIQLRGEL
                     TSGLRTLEAVAHAQETAERLPGGDLRIEVTAVLSAQNGNESGHYRLTRGGLISTAGGQ
                     VRPALQSDRAVLLSPWSRLSSSWAMDLLSTAIEQGKKTDAIAMLRLFDSEIDGLEIIK
                     RFEQGRILVQHRTRGMPSLSTFGAGVHVAMTMAAAVSKARGGLLLVDEIETGIHHSML
                     VEVCSHLFAMAAASDVQVFCTTHSLETIDALVTAAERSQQTDALCGYRLDRSEREHTV
                     RRYQGAKLSRLREGGVDLR"
BASE COUNT         4972 a         7712 c         7987 g         5149 t
ORIGIN      
        1 caaggtctac accgaaaacg tcgaggtcaa agcggcggag acgcgcccgg tgtgggtcat
       61 catggcgagt gacgtgaaga ccggcgcgct gattgtgtcg gcggacgttc ctgccgatgt
      121 agtcgtcgac ggtcagccca aaggccaggc acccgtggtg gtggacaacc tgaccgaagg
      181 cgagcacgtc gtcgaagttc gtcgcaatga accaggctcg ccgccgtggc gtcagaatgt
      241 gcgcgtggtt ggcaaccagc agaccaaagt gcatgccaac accacgccgc cgccgccggc
      301 tgccggaacc ctgatggtcg tgaccagcgt cggtgatgcc gatgtgcaga tcgatggcgt
      361 atcgaagggc gcaccgggtc agccgatcag cctgtcgccg ggtcagcact cgatcacggt
      421 gtcggccaag ggtcacaagg ccgtcaccaa ggtcgtcgaa atcgaagctg gcaagccccg
      481 cgtcgagcgc atcgagatgg aaggcgacgc cgtcacccgc aaggtcggcc tgattcgcat
      541 cgtgatggcc aacccgatcg acggcgcgca gtactacata aatggccgtc gctacgacga
      601 aaccgccgcg ctgtcggatc agggcgtcga aatcgcgtcg ggcaaagtga tcgtcgtcgc
      661 caagaaagac ggctttggcc aggacagcaa agaagtgacg ctcgctgctg gatcgaccga
      721 aacggtgacg ctgaacctgc gcaacgtcgg caaggtctac atcgcgtcgg aaccgaaggg
      781 cgcttacgtg acgctggacc gtgtgctgat cggccagacg ccgctgaccc gcgaaaacat
      841 cggccagggt ccgcacaccg tcgagttcca aatgcccggc tttgagcaaa aggtcgagca
      901 ggtcaatgtt cgctcgggcg aaaccgagca agtcagcgtg atgctggtga agatcaaaga
      961 gaaggacgaa gatcccaaag aagttcgtcg cgggctgtcg tcgttttcgg cggtcacgaa
     1021 caagtttggc agcttcaccg cagacatcgg cagtggctat ccctggatcg gcaatgtccg
     1081 tctgaccgtc ggtgtgtgga agaccaaaca cagcgccctc ggcgagatcg gtctcgacat
     1141 cggcaccgag ttccgcagca acttctatag cgatcacgaa gccgcgggca cgattcgctt
     1201 ccagatgatg cgcaagggtc cgttcgctct cggcctgcac atgctcgtcg gtggtggcgg
     1261 tggtccgcgt ggccgcaaca gctttagctg ggaactgggc gtcccggtca cgatcctcgc
     1321 ggggagcatg gtcaagctga caggcaaggc gtacctgcag gtctactcgg atcgctactg
     1381 cccgtcggag gatgcgattc gggaaatgta caaaaacgat cgcaataagt ttgtccgcat
     1441 gggcaacccc gccgatacct cggatggtgg tggcgccgag cacaccggcg atcgctgcgt
     1501 cggtcgttcc tacgatccga agatcagctt cccgagcccg atctacgctg tcgagcagta
     1561 caacggcacc cgcaagtacg atgcaagcct gcccgacttc caggtggacg gcaccggcgt
     1621 gctcgagcgc ttcgtcagtg tccgcggcat gctacaggcg gcggtggagc tggccctgac
     1681 ctcgaacgtg agcttctacg gcatcctcga aggcgcgccg ttccagaagg agcgtcagct
     1741 cttcaccgac aagttcaacc ggctgttccc gatccacgac acgccgatct acggacggct
     1801 tggtctgacc gcgaagttct tctagccgcc gagcccgcgt cctaagcggg cctttcccgt
     1861 cgctttgaac ggcgtccgcc gacttcccct cgacagcgct ttctaaaaga accgcttcag
     1921 atccatgccg ctatacagct tggcgacttg atctgcatag ccattgaagg gcagggtttt
     1981 ccgtcttcgc agctcgccga tccgtcgctc actggcttgc gtccagcgcg ggtgatcgac
     2041 ctcgggattg acgttggcgt aaaagccgta ctcgccggga gcttgcttgt tccacgtcgt
     2101 cactggctgc tcttcggtga agcggatcgc gaccacgctc ttgcagctct tgaagccgta
     2161 tttccacggc acgatcagac ggaccggcgc cccgttttgg ttgggcagca cctcgccata
     2221 gacccccaaa cagagcagcg agagcggatg cattgcttca tccatgcgca gcgcttcgac
     2281 atacggccag tcgagcgccc agctcttttg tccgggcatc tgcgccgtgt ctttgagcgt
     2341 cacaaactgg acgtacttgg ctttgctggt cggctggcag agcttcacaa gttccgacag
     2401 ggagtagccg atccacggca ccaccatcga ccacgcctcg acgcagcgga agcgataaat
     2461 tcgttcttcg agcggcgcca gcttgagcag ctcctcgatg ccgacggtgc gcggcttctg
     2521 tacttcgcct tcgattcgca ccgtccacgg gcgcgtcttc agcgtgtggg cattttccgc
     2581 tggatctgct ttgccgagcc caaactcgta gaagttgttg tacgtcgtca catctttgta
     2641 ggagtttggc gcttcagaga cggaaaaatc ggctcgtttc gtcgctgcga gcttctgcgc
     2701 cgattccgct ttcgatgtct tggtctggcc aaacagatag acgccggctc ctaacgacgc
     2761 gccagccatc ccggtgccga tgagaaagcg tcgtcgactg agaaaatccg cccgcggggt
     2821 gatttcgcga cccaagatgt cgtccggttt tttgatcagc aaaggtgact cccgtccgct
     2881 gctgtttttt gggcggacat ttgatcattt tagattcctg cgacgggtgc tgcactccga
     2941 ctttgtgatc gcgaggccgt gctagttttt tgccaaaccc acaaaggagt ctgtatgcca
     3001 gcggcgattc ggcgattttt gacctttatc ggcgggatca ctgtgttgtt tgtgattctg
     3061 ggcatcgtgc ttgtttccac cgccacgcgg aaaaaagcgg tgccacagca ggtgattttg
     3121 gagctcgatt tggagcgcga ggtcgtcgag tatctgcccg acgatccgat cgcgcaggcc
     3181 gccggcgggg aacgcatcgt gctgcacaac ctgacgatga cgctggagcg ggccgagcgg
     3241 gatcagcggg tggttgggct ggtcgcgagg cttggcgctg cgaaagtccc cgtcgcgacg
     3301 ctgcaagagc tgcgcgatgc gatcattcgg tttcgccaaa agggcaagtt cgccatcgcg
     3361 ttttccgaaa cgtttggcga attccaaaac gggctcggtg cctactattt ggcgacggca
     3421 tttgacgaaa tttggatgca gccgtcgggc gacattggtc ttgccggact gtacctagag
     3481 tcgccgtttt tgcgcggtgt gctcgacaag ctgggggtga agccgcggct ggatcagcgc
     3541 aaggaataca aaaatgcgat gaacatgttc accgagcggg cgatgacacc agcccaccgg
     3601 gaagcgatga ccaaaattgg cgagagcatt ttgggtcaaa tcgtccgcgc gatgggcgag
     3661 tcgcgcaagc tgtccgcaga cgaagtgcgg gcgctcatcg acaaaggacc gctgcttggg
     3721 gcggaagcgg tgacggcgaa gcttgtcgac aagcttggct atcacgatga attttatgcg
     3781 gcggccaagc agcggtcgtc gggggctgag cttttgtacc tgcacaaata cgccgagcgc
     3841 accgagcctt tgcacaagag cggtccacgc atcgcgctga tctatggcgt cggtggcgtt
     3901 tcgcgcggga aaagctcggt cgatccgctg tcgggtgatg cgacgcttgg ttcggacacg
     3961 gtcgcggcgg gtttccgggc ggcgatcttg gacccggaag tcaaagcgat cctgtttcgt
     4021 gtcaacagcc ccggcggttc gtatgtcgcg tcggatgtga tctggcaaga ggtcggacgg
     4081 gctcgtcgcg ccggcaaaaa agtcgtggtg tcgatgggcg cggtcgctgg ttccggtggc
     4141 tattttgtcg cgatggctgc ggacaagatc gttgcggagc cggcgacgat taccggatcg
     4201 atcggtgtgc tcggcggcaa gctggttcca gcggagctgt gggaaaaaat cggtctttcg
     4261 ttcgatgcag ttcagctggg caaaaatgcg gcgttctttt cgatgaaccg cgactattca
     4321 cccgaggagt gggcccgtct gcaagcctgg ctcgatcgcg tctacgacga ctttacgagc
     4381 aaagtggcga cggggcgcaa gctcgacaaa cagaaagtct tggagatcgc caaagggcga
     4441 atttggagcg gcgaagatgc caaagggctg ggtcttgtcg acgagctggg cggacttcac
     4501 accgcgctgg cgctgtgcaa gcagctggcg gggatcgcgg acggacaaga tgttgaacta
     4561 aagacctatc cgcaggaacg tgggccgctc aaagccatcg tggccaagct gacgcacaaa
     4621 gatcccgata gctcggaaaa agaaggggcg caggtgcgga tgcaaagtga tgcgctacag
     4681 tcggcgctgc gcgtggtgca gatggcgaaa cagctcgggc tcgtcggcag ccaggatgtg
     4741 ctccagatgc cagcggtcga gctgggcttt tagatttccg tcgggcggtt acagcgattc
     4801 ggcgagtgcg cgggctttgt ctaccgccac ttgcagtgct gcgacgcctg cgtctggacc
     4861 cgcgagcatc atcggctctg cgatgacttc atggattgga ttgagtccaa ttagacccaa
     4921 gatggtgcgc aggtaaggaa cctgaaaatc gagcgccgcc agctcggttc cgggcgtgta
     4981 gatgccatcg cgtgatgtca aaagtaccgc aggccgtccg ccgagcagtc cgcgaaactg
     5041 accatccaga tactcaaacg tcagccgacc ctggaccacg cagtcgatcc agcgcttcag
     5101 gtgccaggga atcgaaaaat tccacatcgg tgcggtgatc acgaccatgt ctgccgagtg
     5161 aagctgatcg gtaagctggg tcagcgcggc ccagcgttcg gcgccccgct cgtcgagctt
     5221 gccttcccca tagagcattt caaactttgc ggtgatgtcc cactcatcga acaccggcag
     5281 gctgcggtaa tcgtctgcca ggttgagcga gatccgcctt cgctcgggat ctttttccag
     5341 ccaggtatca aagaaggcgt tgtagagctt gcgagtccgc gacgcttgtc cgcgcggcag
     5401 gacgttgaca tcaagagcga tgggcataaa gacctcgtgg ttcgttacgc gaccatcgag
     5461 tgtggtcggg cgtaccagaa ttcaaagtgg gtgccgccgc cgcttagcgt ggtgcgaaag
     5521 ccctgcgcca gattgagcgt gctggccagc cagctcggcg atggaatctg tggtggggaa
     5581 aacgccgtga gatcggcgag cgaatcgatc aagaagacga gcgcgctgcc ggtgtctttg
     5641 atttcgacct tgccgccgcc tttttcgatg atgaatcgcg aaatgtccga cagcgccgac
     5701 tgcagcttca tgctgtcgtt ccagccgatg ccgaccgccg tggccatctg gtgaatcagg
     5761 ttgcgcaccg cgatgagcga gatggcgtcc ttgagcgaga tcacgcgctt ttccgcagtt
     5821 cggcagcttg cagcctcgag catcgcatgc tccacgcggg tggcggtctg aatgcccgtg
     5881 ttgcggagca gtcgcacaaa gtgattgatg agtgtgctgc ggcgctcggt ggtaagttcg
     5941 ctaagaacga tgtcggcgcg ggtgccgagc gcggtcaatg ccgcatcaat cactgcaggt
     6001 tgcacaaacc tgactgcgat gccgcgggcg agctgtgcga gcgtgacgtt cgagcggtcc
     6061 atggctcgta atcccaatgg ggtcagaaat tatggtacga caaatcgcaa ttcctgatcg
     6121 attggataat gaaccatttg tcaaacactg ccgcgtcgga cttgggctga tgtggtcggg
     6181 tagcggatgg aaacgccaca gcgctgtgct gttttcgggc gtgactgcgg acagagcacg
     6241 gacgtgctgc ggactggcag caagcgggac gttaaagtgt acttcttgct gccggtattc
     6301 gactgtccgc tggggtctgc gcagcgctta gctttttgcg taccacgcgc gcacagaagc
     6361 atgatcgcgg actgcatcgt ggacgcggtt tagcttggga tacgcggcaa aaatcgtcgc
     6421 cggaatgtga tcgactttgc cgccggcaaa ccagcgcact gccaagtaga gcttcagatc
     6481 aacgacattc agtttgtctt tggcaaagaa gggcccgtca ccaagctgtc tctctgtctg
     6541 ctccgcccaa accggaagat aggaagcgac aagtccttcg cgcacgcgcc gcttttcttc
     6601 tgggtcggca atccgaatcg tcggtccgac gtgggcgcgc aagtcctcga catggtgcat
     6661 cacggcttcg tgccgcgccg cctcgaacgg atcatctgga tgcagaccgt ggcggcgtcc
     6721 gatcaggacc agaatcgcgt tggtctgtgc cagcaaagga cgccccggtt cctcataggt
     6781 gggaagtccg ccaaacggag tcgccggctt gagcgcgggc cagtccgccg gctttatccg
     6841 cacgtcctcg aaatccaccc ccgcaatgtg cagcgcgaga cggcactctt caccacggct
     6901 tacgggcgca tcaaagtagg tcagcttggg tttttgcatt ctctgtttgt actagagaca
     6961 gcgaatcttg acgagcggat tccaagccca agtcgtgcag aagggaatgc gcaggcgtga
     7021 agtgggaatg cgcagccgtg aagtgggaat gcgcaggcgt gaagtgggaa tgcgcaggcg
     7081 tgacgtggga atgcagttac ggagacgtgt tggcgatgag cagataggca ggactttggc
     7141 tgctaccgac caccagatcg accgtcccat caaagttcac atcgcgcgcc aaaagcgact
     7201 gtgcggtgcc cggctgctgc gcggacagat caatggtgct acccgcgctg agcgttccac
     7261 ttgcattccc aagcaacatt ccgagcatgt tttcggtcgc gctgctcacc aacagatcga
     7321 gcttgccatc acagttgatg tcgacaagct gaacgtgacg cggaatcacc cccgatggca
     7381 cggtcaccgc gctttggaac agaccgccgc cctgcgctgt gagtacggcc acgttgcgct
     7441 gcccgacaag tccggcgacc acatccggca gaccatcgcc atttatgtct ccagcggcca
     7501 ctgaatacgc ggcggctggg actttgtttt gcgaaagtcg cgcaaatgtc ttggccccgg
     7561 ttttttgcca cacttcgaca aactgactgt ttccaccggc cacgaccagc tcgggctgtc
     7621 catccgtcgt cagatccgca agcgtgacaa gctgtgactg gcccggcagt ccatttgcca
     7681 cgacatcagg ggaaaggaat ccggtagtgg cgcccaaaag ctgatacatc gtgccgctga
     7741 tccccacggc gactacatca tcgcgtccgt cggcattgag gtctctgctc gcgattccgc
     7801 gcagcggttc aggcatcgga atgctctgcg tagacaggac tcctttgcca ctatttagca
     7861 gcagccacag ccggggattg ctcgcatcac tgaccatcaa atcgatcgct ttgtcgccgt
     7921 ttgcatcgcc gatggcggct tgaaacagcg tgcctgccaa gctgaccgga gtcgtggtcg
     7981 taaacgatcc atttccgtcg ctgagaaaga gcctaacgct cgctgattcg atgccgatga
     8041 tgtccggatc tccatccgca ttgaagtcgt cgatgacgat ctgctgcacg ctgctggtgg
     8101 gggaagaggg aaggatctgc ggctggctat caaatgtcag cggatcgagc gaataggaaa
     8161 acgcactctc tttgctgaca cgctcgaaac acgagctgat cacagtcacc ggagacttcc
     8221 cacaggaatt gatcgtcgcg cccagcgtgc cgcgaagctg cgtcggtgaa tcaatctgga
     8281 tgttgtcgag aggcgttccg tcgatctcga cccgcatgct gccttggaaa ttgctgccgg
     8341 tgatcgtgat cggggtgccg ccgccacgac tcccaaactg cggatgtatc gcatcaaagg
     8401 atatctgtgg acacgccgga ggcatcagcg gaacgcaccg ctccgtttca tagtcacact
     8461 cctccaggtc gccacaaatg gctggtgcaa tcgcgcagtt tttcggattt gcgacaagct
     8521 gggctttcca caagtccgta cagccactgc tctgcacaag ggagcacagt ccgacgaaag
     8581 caaccgcaag gcgagtcgtc tttggctgtt gcatgaagcg gactcctggc tatttgttgc
     8641 ctggcgcgat gctcagcatg ctgttgctgt tgagtacgtt acgaccgacc agcagatcgg
     8701 gaaatccatc cccattgata tcggccacgg caagcgcgcc gtttggctgc gtgctttgcg
     8761 gggctttggg agtgaacgtc ccattgccgc cgctatagaa aaacagggtc tttggattgg
     8821 gtccaaactg attgatcaga atgtcctgaa tcccgtcgca gttcatgtca tacgtggaaa
     8881 gctcggtatc aggtgtccca atcaaatccg gaatcggaaa cgtcagggaa tcccatcggg
     8941 tcggacggtt cagcgcgaca accagggttg ccgaagtatt tgcctgcgtg gaaaggaatg
     9001 ccagatcgtt ttttttgtct ccatccagat cggcagaaat gacctgacca aacatgattc
     9061 ctgggacagc cagcggaatg ctcacgggta gggttttggt tccatcccaa ccaaagatgg
     9121 tcaggtccga tgaactgctg gatgtaagaa caagctcgag gtttgggtcg ccacccagat
     9181 cgactccggc gatgccagcg acgatgttcg gaacaaaaaa ggggcccacg cgaccaaaac
     9241 cagatcctag attggcccga tgcaggacat agagctcgct ggactgggtt cctggttttg
     9301 acgctgcgta aatcacttcg tccagaccat cgcgatcaag atcagcggtc gtcatcgcca
     9361 agagcggata gatgctatcg aaggagtttt ctgtgaccag tccgttgtaa tactggagga
     9421 gtttcacttg gtgattcgct gcgaaaccca gcacgatgta gggggcactg ctcatgccag
     9481 ttgttggtgg tccgctctgg gcaatcacca gatgcgacgg agctggggaa tacacctgat
     9541 ccggcatcgc aatcgtggtt ccgatatttc tggccagcaa caccccggtc tccgtgcgaa
     9601 aaagcagatc gggccacatg tttgtgtcct gatccaaatt gccgaccagg atcttgttta
     9661 ccgggcttga gaccgcgccg agtggcagga aactgttgct tgtgaatgga tcgaagagat
     9721 acgtaaacgc tgcggtcttg gcgattttat caccgcgcgg attgctaagc tccacgtcga
     9781 ccggcatcct gcagcgcagg ctctgtgtgg gctttggcgc gattcctgaa agtacggttc
     9841 ctgttttgtc aaacacggga ccatcgatca aaagtccgcc aaattttgct gccgtgtcac
     9901 ttgccaatcc ggcccctttg atcgtgacct gggtgcctcc gtacagcgat cctgtcgtcg
     9961 gtgtgattcc gtcaaagcgc agcggggtac tttcacacgc gccgcttgtg gaattgcaga
    10021 cttgataggc cgcgcacatg gcgtttttgc acggatcttg cggcgcggtg ctgatggtga
    10081 agtctttcca cagcgaatca caggacgccc aaacggaaac agacagtgca ccagccagaa
    10141 gcagagactt cgatttgatg tgatcgatgc gagaacggat aagcataggt cgactccttg
    10201 taatcggaag cgtcaaacgt aagttaggga atgctcccca tcgcgacatt caggccggtt
    10261 cgctggatca caggatcgcg catcacaaag tcgggcagcg tgtcctggtt taagtctgta
    10321 aacaggaacg atcctccggt gttttggagt gtctgtttgg gcggataatg cccctgtccg
    10381 tcgtttagaa aaatcgtgat gttgttgatc ggactcaccg acgctgacgg gcccagaatc
    10441 aggtcgagca gtccgtcgca gtttacgtca taggcctgca tgggcggtcc gggttcagtg
    10501 acgccgccgg ctgacttgat cgttgttggt tgttcatcga tcttgataag cgatccgttg
    10561 ccttggtttt ttaggaaaaa gacgcgaccg tccgtaccaa gcgccgccag atccgttttc
    10621 ccatcccgat cccagtctgc agcgaccagt gagcttacgc cgacgggcag cgtgatggcc
    10681 ggcgctcctg cataggtgag cccgaaactc cccgtgcttt ttgcatagac caaactggtc
    10741 gagtttgctc caccaagaac cacatccgga aatccgtccc cggtgatgtc gagaaccgcc
    10801 atggcaggca acggagaccc gccaagattc acagtagacc cggcactgac gttgccggtg
    10861 gcagactccc tggcgggcgt tagatagtag ttcgttgaag acgtgttgct gccgtagatc
    10921 acgacttcat cggttcccac ggcatccagg ttgatggtgg ccacatcctt tggcaccgtg
    10981 ccgttattgt aagccggcgt gaacgatgaa accgtgttca caaacagcag tgcgtttgtg
    11041 ttgggatcga cgatggctgc ggctgttcgg cctggtagtt ggaaggcgcc gaccgaaaag
    11101 cggatgggtt ttgaaaacag aagtccggtg ttctctgccg gcccagcagc tccggcgttg
    11161 ttggaaacga agtacagtgt cttggggctc gacacaatca gatccggaaa gcgatctcct
    11221 gtgaattccg cttgtgcaat ctggagtacc tcctcgttta ggtttgcgag cgcaaaaggg
    11281 ggggcgttgt gatacggctc aaacttgtac agaaattccg ccggatggag cgcagctggg
    11341 aatccggttc gagtcacttc gacattgacg ggtccgcatc gacctggtga aaccggcgtc
    11401 agaccgtgaa tcacagttcc gtttccttgc accatcggct gcacaagcgg ctgacccgca
    11461 atcttgacca cggtgtcggt tgtgaatcca gtgccgcgca gatcgaccgc atcgccgccg
    11521 cccgtttttc ccatcgaagg gttgattccc gtgtaggaaa aatagtctga gaccgtgctg
    11581 gcttcacaca ttccggtgtc gcgattgcag acctgcgctt ggctacacag agtcggcgtg
    11641 ttgacacagg aactggggtc tcccgtcgta aaaccattcc aaagggaact gcatccactt
    11701 tgggaaagtg cagacagaag tgacagtgtc gcgatctggg tgaaggttgg tctttgaata
    11761 tgaatcatat gaaatgcctc tatttagtcg gtctgtttgc taagcgagat cgaaagacca
    11821 ggaagcagaa gctgggccgc tggtttgggt cgcgacacct tcgacggaat gctgatcaga
    11881 aggagtcctc cgaccgcagt gagaacgcct cctgtggtca gcgcaatgcc ttgtgtcttg
    11941 gtgtcgtaat agcgacggca ttccacaccg gggggcggat tcggaacgca gatgccatca
    12001 aacgcaaagg ctgacattcc gaaaccgaac atcacgccgc ctcctacgac aagtgcgctc
    12061 ccggtgagaa tccgccacag tggccagcgg tacttgcgtt cggacggccc caccgatcgg
    12121 ggcaataccg aaagcacttt tggggccgcg ggcggcggtt cgtattcgct gggatcgggc
    12181 tctggttcag cggggctcgg ctcgacggcg gggatctgtg tcggcagttc aatccggggc
    12241 gcagaaagcg gcgcgtgtgc ggcggtttgg gcagcgccga cgttgggcac acagagagag
    12301 gccgcaagca gaagtacgaa gtagcgcatc gcgtggcgag gccccccaag acccctttgt
    12361 gcaacttcct attttagttc gggacgccgc gcgttgcctg cttgtcgttg cgaaagttga
    12421 cacggccttt gcgcatccgt cacgctgatg gggtgaagat ttacctgatt cggcatgggc
    12481 atgcggtgga tgcggagctt cacctggccg atggagagcg ctatctaagc aagaaaggtc
    12541 gccgcaccac gcgcgaggtg gggcgggcgc tgcgcaaggc ttctgtatcg gtggatgcga
    12601 tcctgaccag tccacttgtg cgggcggtgc agacggcaga gcttgtcgct gagcagcttg
    12661 gctttgaagg cgtcattgaa gttctgccct cgctgatgcc gatcgggccg attgaaacca
    12721 gcgccgaagt gatccgcgag cagtcacgaa ccgtggcagt cgtcggacac gagccgagca
    12781 tttcggcgct ggctggactt ctgaccggcc agggtcactt tccgccgctg cgcaaagccc
    12841 aagtggtgtg tatccacggc gatcgcgcgg tgtggacgat cgatccagat cgactcgatc
    12901 cggtttcgct ctgacggatt cgcggggact gttttcgccc gacttgctgt ccgcaaacgc
    12961 cgccgccgtc tagcgggccg gcagcagggc caccgtttcc tccggcagct cgagcagtcg
    13021 ccacagatac cagcttgcga cgctgcgata cggtgcatag ggctgcccca gttcctgcat
    13081 ttgcttgggc ggtggcagat catcgagccc gtgcagtcgc tgcaccgctt tgcgcacccc
    13141 caagtccccg actggccaca cgtcgagccg ccccaggtga aacatcagga acatctcgca
    13201 ggtccagcgc cccagcccgc gcacgctggt cagccgatcg atcaccgctt catcgtcgag
    13261 cgcatcgaca tccgacagaa agtgcggatc gtctatcagc ttctgacaca gatcgcgcag
    13321 gtacgacact ttttgccctg acagaccgca gccgcgcagg ctctcgtcgg gtttttgcat
    13381 gatctgcggc gctgtcggaa attccccggt cggccacagc gcgcggaatc gaccaaatat
    13441 cgtcgacgcg gctttgcccg aaagctgctg aaacacgatc gatcgacaca gggcttctaa
    13501 gtgggtgcgc tgccgaggcg gtcccatctc gaacggtccg acctgatcga taagccacgc
    13561 catcttgcga tctcgcttgc gcagcgtcgg cagaacctgc tttggaatcg gcataaactc
    13621 aggctttttt tcggccttca tcgcaacacc tttgggattt gtgggtagct ggggtcaaag
    13681 ggcgctgatt ctagccttgc gcagccggcg tggctgcggt acacgccctg ccaaccgatt
    13741 tttccgccat gaccgtctcg acaccgcatg ctggggctgc tgctttcacg gaacggctgc
    13801 ttcccggcga acgatcccac gacgctgcca tgatgcgtct ggcgatcgca gcggcagaga
    13861 cagcgcgcgg tgcgacaagt cccaatcctc tcgtcggtgc cgtcattgtg gatgagtccg
    13921 tttccccacc acaggtgctg gcaacgggtt ttcatgcgca ggctggacgc gcccacgccg
    13981 aagtcgatgc gctgcaaaag ctggcccaaa ccggcgcaag tgctacgaaa aagaccctat
    14041 acgtcacgct agagccgtgt aatcacgtcg gacggacggg caagtgtacc gatgcgatct
    14101 tggcggcggg acttgctcgg gtggtcattg cgacagccga tcccaatccg cacgttgccg
    14161 gtggtggggc agggcggctg cgcgaagctg gactcgacgt tctcgtgggc gtgtgcgagc
    14221 cgcagtgtca gtggcaaaac cgcggctttt tgcgctggct caagtcgggc aggccgcagc
    14281 ttctgctcaa agcggcgatt tctctcgatg gaagaatcgc accacaggcg ccgccggaca
    14341 cggtgattgg gccccgctgg ctgaccggcg ggcccgcgcg gcaaaaagcc catcagctgc
    14401 gggcggcaag tgatgcgatc ttggttggtg ccgggacggt gctcgccgac gatccgcagc
    14461 ttaccgtgcg gcttttcgat cgcgaagtcg tccgtcagcc gctgcgggtg attttggatg
    14521 gtgcgctgcg ggttccaacg accgcgaaag ccctgggtcc ggggtcgctt cttgtgacct
    14581 gtgaatcgag ctttcaagcg cagccggacc gggtcgccgc gctgcaatcg atcggaaccg
    14641 aggtcattcc gttgccactc ccgtcgagtc ccgatgtggc gaaccgacgt ggtgcggcga
    14701 ccgatgtcga tctacatgcg gtgctgcgcc tgcttgggca gcgtcaggtg ctctatgccc
    14761 tgtgcgaagg cggtggcgtg ctgcacggtg cgctgcttga atccggtctg tgcgatgaag
    14821 ccgcgctgtt tgttgcgccc ttgtttttgg gcgacggcgg ggtgccgctg ctgcgtcact
    14881 tggcctatcc cgacgtgacc gcggcggcgt ggatcgaaac accgcagtac tcgcagcttg
    14941 gcgacgatct gtttgtcacg ggggcgctgc gtcgcggcgg ttttgcaccg tctgcctgac
    15001 gggcaaaagg agtcagtttt gtttaccggc ctagtcgaag acctcgggcg aatcatcgaa
    15061 gtatccccgc tgccacgcgg aagtggtcag cggatcgggc tttcgtcgcc cttgctttcg
    15121 cagccgttgc cgcttggcgc gtcgctggcc gtcgatgggg tctgtctcac ggtcgtcgct
    15181 gcgtcgcccg gcttggccag tgtcgaggtc ggtcccgaaa cccgcgctcg caccacgctg
    15241 tccgatctgg cacctggacg ggtcgtccat ctggaacgtg cgctccaggt atcggatcgc
    15301 ttgggcggtc acatcgtcac cggccacgtc gatggcctag gtcacgtcag caagagcgtt
    15361 cggcgcggcg atagctggga tgtcgaagta aacacccccc aggaactctt gcgctacatc
    15421 gtcgaaaaag gtgcgatctg catcgacggt gtcagcttga ccgtcaacag cgtctcggcg
    15481 cgcggttttt ctgtgtcgct tgtgccgcat acgcaggcgc acacccattt ggcgcaaaaa
    15541 tccgtcggcg gtgcggtgaa tctggaagtg gacatcatcg ccaagcacgt cgagaaactg
    15601 atagccgctt atcttccgac gcagccttcg gggctgtcgc tcacaaagct caaggagttt
    15661 ggttttgcac gcggggacta gtacgacgga acgagctgcg cgcgtaaagc gggcgctcgc
    15721 tgatattttg gccggcaaaa tggtgattct cgtcgacgat gaggatcgcg aaaacgaagg
    15781 cgacttggtg atggccgcgg acaaggtcac tcccgaggcg ataaacttca tggcgcgctt
    15841 tggtcggggg ctgatctgcc tgacgctgac cgaggagtgg gccgatcact tgcagttgcc
    15901 gctgcaaagc tcacgcgcgg ggggaccgcc gctcgggacc gcgtttaccg tcagcatcga
    15961 ggcgcgccat ggcgtcacct cgggcatctc agcagcggac cgggcgcaca cgatcttgac
    16021 ggcggtggcc aagaacgcca agcccgaaga cttggtgacg ccggggcaca ttttcccgct
    16081 gcgggccaag cgcggcggaa cgctcgtgcg gaccgggcac accgaaggtt cggtcgatct
    16141 gctgcgactg tccgggctta cgccagcggc ggtgatctgc gagatcatga acgacgacgg
    16201 cacgatggcg cggatgcccg atcttttgcg ctttgccaag cagcacgacc tgctggtgct
    16261 cagcattgcg gacatcatcg aacatcgttt gcagacggaa tcgctggtcg aaaaagtcga
    16321 aacattcgag cttacaccga cgataaacgg tcaaaaagcc aagcggtttt ccggggcgct
    16381 gtttcgcacc gcgattgaac cgacggaata cctggcgctg tggatcggtg atctgtcggg
    16441 cgacgatccg gcgctggtgc gggtccagac cgcgagcctg ccgggcgatg tgttcggcgc
    16501 gctcggctgc gacagtgggg cccagcttca ggcggcgctg cgcatcatcg acagtgccgg
    16561 acgtggcgtg cttttgtacg tgtttccgac ggggcgtttc aatgtccggg acgacctgcg
    16621 ggcccacctg ctccaccgta ccgatgtgat tccgtcgggg aacaagctgc gcgactttgg
    16681 gctcggggcg caagtgctgc gcgagctggg cgttcagtcg atgcgtcttc tgaccaacaa
    16741 ccccaagaag attgccggac tgaccggcca tgggctaaag ctcgtcgaaa gcatcccgct
    16801 tctgccccag ctgccctagt ttggccgtcc gttacctgat tttgtgagag gaagacttat
    16861 gtcgagtgaa gaccaaacga aagtgatcga aatctcgggt tcgctttcgg cggcccaagc
    16921 caagatcgcg atcgtcgcca gccgcttcaa cagctttatc gtcgagcggc tcatcgaagg
    16981 tgccgtcgat gtgctgcgtc gcgtcggcgg tgcgcccgaa agcatttcca tcatccgctg
    17041 ccctggcgcg ttcgagctgc cggcagtcac ccgtcgctgt gtcgaaagcg gcaagttcga
    17101 cgctgtgatc tgcctgggcg cggtcattcg tggcgccacc ccccactttg actacgtcgc
    17161 tgccgaatcg agcaagggca tcgccagcgt cgctgcgcaa gccaccatcc cggtcgtttt
    17221 cggcgtgctg acctgcgaca ccatcgaaca agccgtcgag cgcgctggca ccaaagctgg
    17281 caacaaaggc gctgacgctg cgatgacggc gcttgaaatg atctcgcttt tccggaagct
    17341 cggacatggc taaagcaagc ggaaatcgtc gtcgggctcg cgagagcgcg atgcagatcc
    17401 tgtgtgtgct cgataggcag cccgagcttt tgccgggcca ggcgacggcg ctctattttg
    17461 cccacttgct cggcgacgat ggcgaatcga gcacgtcgat cgacgaacgc acgctgccga
    17521 gcgactaccg tctgtttgcc gagacgctgg tttccggcgt tcgcaaagca caaagcgaca
    17581 ttgatgcgct gctcagtcgg tcgtcgaaga actggcgact gtcgcgcatg gcggttcccg
    17641 atcgcaacat tttgcgactg gcctgcttcg agctgatgtt ctgtcccgaa gtaccggcgc
    17701 gtgcggtgct aaacgaagcg atcgagattg cgcggcgttt tggcaccagc gactcgtcat
    17761 cgtttgtcaa cggagtgctc gatcgggtcg tcgtggagct gggccggcga caagacaccg
    17821 agcacgcgac cgagtcagag tccactcctg cgtagtctgc ggacaaaggt gagggcagct
    17881 gtgaacaagg cattccttct tctgtttttg gcctgtatcg cctgtgcgtg tgaacccttg
    17941 ccgtacagtc cccatcgggc caccgaatcg ccgctgccga aaaatgcctt tgttcacttg
    18001 gtcgtcgtca gcagtccgtc gccgtccgtc gaagtgtcga tttcgaacca agtgcgcgcc
    18061 gtcgtgccac gacgcggcat tagtcctcgg atggagctgt cttctggcaa ctatccgctg
    18121 ctgattaccg gcggtggttc gacgctgtgc gagtcatcgc ttcagcttct gccgggcagt
    18181 gaaacgctgc ttatcgcgag cgcgcaaaat accgacgaca aaaagtgtcc atcgcttgtg
    18241 ctttcggcgt atccgcttgg gcagcgcgat ccgaagcttg cgcgggtgcg acttttgcac
    18301 agcgcgcttg atgcgccgat gctggaactt gtcggtccgt cgggtgagga aatcgtcgac
    18361 ggcatcgccg ctggtgcagt ttcggcctac ggtggactgg cttcgcccgt tgctgccggt
    18421 tcgacactgc ggctgcggtc gcctctcgac aaacagccac tttttgacgt gatgaccgat
    18481 ggactttcgc tcggttctgc cagcacttgg ctcggcgtcg gtgaaattgg cccgctggca
    18541 tcccaaaatg cctttgggct tttgtcattt gacgaagaaa gcggggcgct gcgcgagcta
    18601 tcggcggtcc cttctgcggg ggcacccgac ggcgaagttc ttttgtttca tgccagcaaa
    18661 gaagtcggct cgcttgtcgc gaaagtggca ggaaagccgc tgttttctgc cctttcgtat
    18721 ccgcatgcct cgccgcttgc cacgcttgct tcaggacttc gcacgctgtc gctggaggac
    18781 ccaagcggta cggtctggac gggtccactg cggctgtggc cgggccgtgg ctggctgctt
    18841 tttttgtatg gcactcgcag cgcgccgcgg cttgtggcgc tgccacggcc agcacttgcg
    18901 ccgcagacgg tgtggcgggt gttgaacttg gtcgatggct tggattcggt ggatctgctc
    18961 gacggcgatg atgccatggt gagccagctt ggatttggcg cggcgactgc gcccacgctg
    19021 gtcgatgcac tgcgaccggg gacgattcgg ctgcgcgatc gcggggcggg caatcgctcg
    19081 tgggacattg tgctgactgc gggcgcggcc accgcgtcgc tcgatcaagt ggtcacgctg
    19141 gtgcttttgg ggtcggcttc aagcccgcgc tcggtgtctg cccagcttgt tgtcgagtcg
    19201 cgcgccggtc tgatgacggc accgcctgtg cttgcgcttc cgaccagtcc ttcgctgtaa
    19261 agacagccgc taagacttgc atcgcaaagg ccacttttgt cgccgtggcg tttggcgggt
    19321 tttgcgcatt gcatcgccgg ctgggtcggt gctaccgtct gagcttggca cgttgtggtc
    19381 ctaaaaaacc gcggcgataa gcccccggaa cgtcgatgcc gattcagcaa gcgcaacaac
    19441 tgggccgcta ttacctgctc gaccgtgtgg cgttcggtgg catggccgaa atttttcgcg
    19501 ccaagacccg cgacgcccaa ggcaaagagc ttctcgtcgc tgtcaaacga gtcttggccc
    19561 acctgtgcga agacgatgag tttatccaga tgctcatcga cgaggcacgg ctgacggcgc
    19621 tgctcaagca tcccaacatt gcccgcgtct acgagttttc caaagtcggc agcgagtact
    19681 tcatcgcgat ggagtacgtc gaaggcaaag acatgcgggc gctgctcgaa agggcgcggc
    19741 aaaaccaaga gtggctcgcc gaagacctga ttgcctacat cggtatgcag gttgcgcttg
    19801 ggctgcactt cgcttacgag cagaaagacc gcgacgggac gccgctgcac atcgttcacc
    19861 gggacgtgtc gccgtcgaac gtgcttttgt cgtatggcgg cgacgtcaag ctgtgtgact
    19921 tcggcatcgc caaagccgaa ggcgcccgtc agcagacccg cactggtgtc atcaaaggca
    19981 aagtcaagta catgtcgccc gagcaggcga tgggtcggac gctcaacaac cgatccgacc
    20041 tgttttcgct tgggacgctg ctgtacgaaa tgctggcgct gcaagcgccg ttcaccgccc
    20101 agacggaaat cgagcttctg ttcgcggttc gcgatgccag aaagcgtccg ctgcgcgagc
    20161 tgcgcccgaa catccacccg gagcttgagc gaatcatcga caaagcgatg gagcgccaag
    20221 agaaaaagcg ctgggaaaac ggccaagagt ttgccgaggc gctcgggacg ttccttcagt
    20281 atcactatcc gaacaccaac atggcttcgc tgtcgcgctg catggccacg atgtttgcgc
    20341 gcgaaatcga caaagagcgc gcagcgatgg cggagtttgt gatcgatacg tcgcgctttg
    20401 atgaaaacgt cggtgaaaac ttgctcgccg atgtgctcgg gcccgacgca gagtacaccc
    20461 agtttaccgc cgcatttgga cgccaagaca tcgccgatcc actgccgagc acgccgacgg
    20521 gacccaatcc caatcagatg cgggtgctgg cgtcggtgag catgccggga accgtcgatt
    20581 tcgatatgct gcagacgacg gaaaagccac gctatgaggc ggtggggatg atgccgccgg
    20641 tgtcgccagc ccccggcgaa ccgggctgga tgccgtcagc gcagaccatg ccgcgcgggg
    20701 cgcgtccgtc ccaagcgatg ccgcctgtac cgatgggtcc gcgtccatcg caagccgtgc
    20761 cgcagcttcc gatcctgggc gacacccaac caaacggcga aagcggcttt gaaacggtga
    20821 tcatccagcg tgagccggtg ctcgaaaagc gacccggtgg accgattgcg ccgcccatca
    20881 tcgacgagca gttccacgcc gaagaaaccc gggtgctgtc gcgtgatcag gtcgattttc
    20941 tcaagctgat tccgcagaag tcgtcgcccg cgccgcagcc tgtgcagtcg gcgcaaaagc
    21001 cgttcgagtt tcacgagcaa gcgacgcaga ttctcgacgc acgcagcctg cctgcggagc
    21061 ttcagcagct cttggcgcgc aaaagtgcag agcttgccgc ggcggtgaaa caggcaccgg
    21121 tgacaccgcc accatcggcg ccagttccat cgggaaaagc ccgtgcgccg tcgatacagc
    21181 ttcagccgct cgactctgat ttccacgacg cggcgacgga ataccttgac tggagcccga
    21241 ttgaaccgtc ggcgatagag gaaatgtcgc tcgacccatc gtcggccaac tttggcgcgt
    21301 tccgtcaggg gacaggggcg cacgcgccag ttgcgctgcc gcgtccggcc acctcggcgc
    21361 agccacagat tgcggtgaca cccccagcgg ctgccttgcc gccgcgtccg cctgcatcgt
    21421 cggcagcgcc gatcccgccg ccgcgatccg ccacgggtgc acagccgccg gtcggtctgc
    21481 cgatgccgcc gctcagccca tcggccaaaa gtccaccgcc gccgccgccc gctgccgcca
    21541 agattgcgcc gccgccaccg cgctcggcca ccggggcgca ggtgccagtc ggtctgccgc
    21601 tgccgccgct cagcgcctcg atgaaaagtc cggtgccgcc gccgccgccg agcaagagtc
    21661 cagcccctgc gccggctccg ccacttgccc cgccggcgct tgcgccttca ctgcgcgcgg
    21721 ccacgccggg ggccagacca acgggacttc agccgcagat tggtcgcact cagacggcgc
    21781 cgccgcttgc caaagcgcca cctgcgcccg aaccaaagcc agcgccagca ccgggtcggg
    21841 ttgctgccat ccagccgccc ggcgaagacg agtgggaagc gctgccgcac ctggactcga
    21901 caatgctcgc ccgcgccggt tcagtaccgg tggtgttgcc acgcagtgcg ctgcccgcgc
    21961 gcccgacggg acgggtcgat gcgattccgc cagcaggtca gaagttcgat ccgctcgagc
    22021 acaccatgca aaccgacgac gagcacatga gtccttcggt gggcgattcg ccgctcgacg
    22081 atgaccagcc gacaccggtg cgcggaactt cgctttcgga tatgggccta ggtacagaga
    22141 gcgagttcgc cgaagacacc gacaaaggcg acggacccaa gggctttaac aagcaccccg
    22201 atccctcgct gacgtttgaa ggtgacgacc tagagccggt cgattagtcc aagtcagccg
    22261 ccaaacagcc ccgacgccag gttgatggac agcgcgatga tgctggtgtt gtagacgaac
    22321 gacacgagcg cgtgcaaaag cacgtagcga cgaatcgtgc ggctcaagac ggccacgtcc
    22381 gaggtctgaa aacacatccc aagcgtaaaa gcgaagtacg cgaagtcgag gtcgtcgggt
    22441 ggttcctcgc cggggaacga aagaccacct tcccccgcgc catcttgacg gtagtacagg
    22501 tgcgcataac gcagcgccca cgccgtgtgc gtcagaagcc acgagcttgc caccgccaaa
    22561 agacacagcc caacgagcag cagccgcagc ggtgcgtttg ccaggtgttc cgcctggttg
    22621 ataagcgccg tcgctgccag caggctaaaa ccacacgacg caagcacaat cagccagatc
    22681 agcgtccgcc ctggatcttc cgatgcagcc cgtcgccgcg ttttttgcgg tcccgagcga
    22741 atcatgatcc accacgtcag cgccagcatc gccagcgagc cgctgtccca cgccgacaca
    22801 atccgcacaa caaagccgag ccgcgccggc acaagcaaaa gcgtcaaaag cgacaccgcg
    22861 acggcaaaac cgaagcgtcg gtgggcgcgc agaaaagaca tccacagacg gatgggccac
    22921 ttgggccgcg aatctccgtt tgagggacag tcgtttccgc tgggacttgg caggcttggc
    22981 gaatccgcca cgatggcacg ctctggcaaa gaccgccgcg gcccccggcc agcccagcag
    23041 gcgggcaagt ggacctgcgg cggacgaaat cgctagctgc cgcgctcgga gttgccgctc
    23101 ggcgcgcctt ggtcgccgct gctgtgcgga cggtcgccgc tgggccgacg atcgcggttt
    23161 ggaccgtgac caccgcgtgg gccgccaccg ccgcgccgac cgccatcgtg ccgttcaccg
    23221 cgtggaccac caccaccacc gccgccgcga cggttgtcgc tgtgttccag gttgcgcacg
    23281 tattcaatga agtgcgcgcg gtcttccatc atgtcgccaa accggccacc ttgcgggcca
    23341 ccgcgacgac gatcgccacc accatcatgg cgcggcggac ggtggtctgg atgggcctgg
    23401 ttgccttcct ggccacgccg cggacgcagg ttgcgccgac tttccagcgc cgacgacaga
    23461 agctcacccg ccagctgcga aagctcgacg ccttcactcg ctgcttgcca cagaagttcg
    23521 cggtgcaggc cccggctgac cttgaccgtg aactcgccgc tctgcgcttg ctcatcaatc
    23581 gacggcgggg gcgacttgcc ttgcgccgcg aggtttgcat acagggctgc cagctcctcc
    23641 tcgacgtgga ggatcgcctc ggcgcgggtc atgccttccg cggtgcagtg cggaagctcg
    23701 ggaacgcgcg ccgtaaacgt cttgcgctcg ctatcgaaag agagaatcac ccgatagttc
    23761 ggtctcgaca ttcaagattt cctttggtgc gaaagtgggc agtcatctga aacagactgc
    23821 acggagagac gcccagtgtg tcgtcaccga cgaaacaaag tcaagccttt acaccgccct
    23881 acacccttgc gccgcgtcta gcaacgcatc aaggaacgtc acaagcggcg caagctgcgg
    23941 cagcgacagc gtctccttcg acacccagcg gtgatcatgg atcgagttct gaatcgcctt
    24001 ccccggcttt agaaatgccc ccatcacccc gaccgttgcc ttcttgcgtc ccgccggttt
    24061 gttttcgagc agcttcgttt cgtctgcctc aagctcttgc aggaactcag accgtctatc
    24121 gaccaagcct tgcgcctgcg tcagaagctt cggatagacc gcttgtgcgc agtcgaggag
    24181 caagtcttcc agcgtccctt ttcgcgttgg tgacggcatc gcaaacacgc caaaacgcgg
    24241 cttgccatgc ttgaccactc cgagttccgg tttgtcagga atcgggatct ggacatcgcg
    24301 agcacgcagt tccgctagca gcttggacaa gcgtgcttgt gcgtcctctt catcgctgtc
    24361 cagcacgacc ccaacgccgt cgagtaccag cttgcctagc agaatctgct ccaggtcgct
    24421 ttccagccgc gctgcgaggt ttgcgattcc ttccgcgttg cttacggcca ccatcaggtc
    24481 atgttgtgga tgaccaaaga acagcggcgc cggcacagcc aatcggctga tatcgagcac
    24541 accctgtcgg ttccgggtgg gccacttgaa attgtcgaca aacgtctgcc atcccggcgg
    24601 gagtttcgtc tgttctttga tctgcgccag ctttgcgtgc ttggtcagca gtcgaccgat
    24661 gaacgctgca tcgtggacgc cttcgacgac cagatagacg tttttcatcg tagatcgacg
    24721 cctccctcac gcaggcgaga cagcttggca ccctgatagc gccgcacggt gtgctcgcgc
    24781 tcgctgcgat cgagccggta gccgcacagc gcatccgttt gctggctccg ctctgcggct
    24841 gtgacgagcg cgtcgatggt ttcgaggctg tgggtggtgc aaaacacctg cacatcgctc
    24901 gccgcggcca tcgcaaacag gtgcgagcag acctcgacca gcatgctgtg atgaatcccc
    24961 gtctcgatct catccacaag cagcagcccc ccgcgcgcct tcgacaccgc cgccgccatc
    25021 gtcatggcca catgcacgcc cgccccaaac gtcgagagtg acggcatccc gcgggtccgg
    25081 tgctggacca agattcggcc ttgctcgaag cgcttgatga tctccagtcc gtctatttcc
    25141 gaatcaaaca ggcgcagcat ggcgatcgca tccgttttct tcccttgctc gatcgccgtg
    25201 ctcagcaagt ccattgccca gctcgaagac agcctgctcc aaggtgagag gagaaccgca
    25261 cgatccgact gaagtgcggg cctgacctga ccgccggctg tcgaaatcag gccaccccgc
    25321 gtgagccggt aatgcccaga ctcatttcca ttttgggcag ataacaccgc cgtcacttcg
    25381 atgcggagat ccccacctgg caggcgttct gcggtttctt gggcatgggc aacggcttcc
    25441 aatgtacgga ggcccgaggt cagctctcca cgaagctgaa tggcctcggc tttgggtcgg
    25501 gtgccaccaa agaggagtgg ctctgcacaa ggaaacagct cccacagcgc atgcgggggc
    25561 gtgtcggaca gaaaactgtc gcggctttga atcacgcgcg tccactgtgc atcgtcgaac
    25621 gggcgggcga ggagggcggc ggcttcgagc acggacgact tgccggagtt gttttgaccg
    25681 atgaggacgt tgacgcggcc aagcccttct aggtgcaggt cgcgcagccc tcggtatcct
    25741 ccgatgtgca gatcgtggag cagcatgcgc cctttgttac accgaaccgt cgcaagttgc
    25801 caccttgccg ctcgctgaca
//
LOCUS       DAORGN010000051        25655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_747_length_25655_cov_5.656484, whole genome shotgun sequence.
ACCESSION   DAORGN010000051 DAORGN010000000
VERSION     DAORGN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 25655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25655
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_25655_cov_5.656484"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKL17_07915"
     CDS             <1..431
                     /locus_tag="PKL17_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44693.1"
                     /translation="SRWQSRLLRGKKRAYLHPAGKEAVTLVQPLWRDGDHVLWQLSPL
                     TGRPHQLRVELARRGCPILGDALYGSRVPWADGIALCAVELDFSALPDQSELGLPPSL
                     RLGGDDAVRSGIAWRKAFASKSGHSIHSACPTDGIRTGAG"
     gene            complement(419..1114)
                     /locus_tag="PKL17_07920"
                     /pseudo
     CDS             complement(419..1114)
                     /locus_tag="PKL17_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            1229..2233
                     /locus_tag="PKL17_07925"
     CDS             1229..2233
                     /locus_tag="PKL17_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44694.1"
                     /translation="MRRLIVGLGLCGLLALGGATGCGPRAIVSDLTHHNMSDEDLLQL
                     LPQGQEAVFDIELQSLRAWPPARSLMALLPEGAQRRLRDVAADPLSDLDAVCIGLRGL
                     GTKDVEATLLFRGSPSRNKLWQQMLSRPQSREVTYHGMPIAEVADESLAQLTPQTVVL
                     GSRLQVRQTIDIFRGVDHGARKQPALVAALAMAPTGKAGRPAVLMAILPSSDLRQKAH
                     NAGLLRILDDAQAVAVAMAIGDGFDIGIVASYPDVTMAQSALAFVNAQAAELSLRPLT
                     KALGLERFISPLVAVAVPQSKKRPTPELHLAYRLPGDDLAELLRRIEQLKQLFPSQRN
                     "
     gene            complement(2219..3079)
                     /locus_tag="PKL17_07930"
     CDS             complement(2219..3079)
                     /locus_tag="PKL17_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK44695.1"
                     /translation="MEVTFHGVRGSIPAPGPDTVRFGGNTCCVSVRSESGKILILDMG
                     TGIISLGRKLLGSEFGKGKGHGAILLSHAHWDHIQGFPFFAPVFIPGNRFSVFGPAKS
                     AAMLEGILEGQVNPHFSPLQTMKNLGAMFELLPVPTDNDPPPIFWEGIAISGRPNPHG
                     PSTCMAYRIEENGRSLVYAPDAGYPDAGPSEEVLRLYQGADLLIHDSTYTPEDRSVRR
                     SRGYSSFADAAYAAVRAGVRRLALFHFDQDYSDDAVDSIYSRCRELLDSLGGRHIELI
                     ASREGSSVTL"
     gene            complement(3070..4101)
                     /locus_tag="PKL17_07935"
     CDS             complement(3070..4101)
                     /locus_tag="PKL17_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK44696.1"
                     /translation="MKLRFWGVRGSYPVPGTTTQRYGGNTSCVEVRCASAQDPSAPRI
                     ILDAGTGLRRLGKDLMHGPFGEGKGDAHLLVSHTHWDHIQGLPFFSPLYRPGNKFHVY
                     ARQRDDMHLRMVFALQSDNPYFPVPFDAAAANVQFSELTDDARFEIGPVKVRCTRLNH
                     PWIAIAFRLDYEGASVAYVTDTAPFRDILIEHEFIRQPPKPGEPLAPEDAAKLAAMRE
                     GVVRLCEGAQLVIYDTQFTPTEYAQRPHWGHSCAEDAIEIAREAGAESLMLYHHSPDR
                     SDAQIDELLGQHRHQLARDGARLELHAAYEGMEVDLGKHGMQVGHASSRSMGQGHRGS
                     SSNEEESWK"
     gene            4420..4644
                     /locus_tag="PKL17_07940"
     CDS             4420..4644
                     /locus_tag="PKL17_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNK44697.1"
                     /translation="MRNIGVRLGFVALLLLGGCTVVRPYQREALAERCMAPGFGDKSE
                     VKFRAHWEYARHGTQGGFGDDAGGGGCGCN"
     gene            4647..5948
                     /locus_tag="PKL17_07945"
     CDS             4647..5948
                     /locus_tag="PKL17_07945"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNK44698.1"
                     /translation="MPKPGAGRGLAGGRWAARLCFLCFVLWTGGLRAEQLPQNVSGEA
                     AFRGSYWRDRNTRVLNPTVDLRQQTSSGVSVTGAYGLDAITSASVAAGATTDQPFTEL
                     RHEIGFGLEVPLAGKSTLSGGYGYSTESDYFSHTASLRTKISLLQDNTILRIGGDYTH
                     SSVGKRLGPTGYLNMGILHEAHAIVGVSQVMSRTIVGSLSYELFVLSGYQNNPYRPVF
                     VAGERREFENLPRLRIRHAIGMSGHKMFRFDRTLVPHLTLRPALRILVDSWGTKSVSP
                     ELAVHLPIGPVELRGLLSYFHQAEASFYRSSCGEQPKVFPGAPCYAERGVEFGTATDS
                     NGNETTFMAYTSDPKLGTYSTWTTELQIKWRMTVFSHLGSVGSLLSRSAIELSGGMWF
                     AQDAIGNQFGIPLVGNDQVGVAGCSALCGAGFAHLGLYVPL"
     gene            5992..6492
                     /locus_tag="PKL17_07950"
     CDS             5992..6492
                     /locus_tag="PKL17_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK44699.1"
                     /translation="MKQPITLVVNGEKHDTLCTPNLTLLEVLRENLQLTGTKHGCELG
                     ECGACAVLLDGEPVLSCLVLALECESRAITTVEGLKRGPVLHPLQSAFADHGAAQCGY
                     CTPGILVTAKALLDKNPCPSRSDVKEALSGNLCRCTGYLQILEAVDAAAQVLREEASS
                     QGGAAR"
     gene            6489..8942
                     /locus_tag="PKL17_07955"
     CDS             6489..8942
                     /locus_tag="PKL17_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK44700.1"
                     /translation="MSHRPLHIIGKPRRRVDGRAKVSGETRYADDISLPRMLHCRLLR
                     SPLPHARIVSIDASRALSQPGVHLVLTGQDMPTTFGILPVSQDEHALCLHKVRFVGDP
                     VAAVVASSELVAEEALSLIDVQYEPLPTIASPEEALATEDPRIHEYGDQGNIHKAVAM
                     SFGSVDDGFAQADHIFEDTFHYAGNTHLPLETHAALAALDPDGKLTIWSSTQTPHYAH
                     RAIAKVLGLPPSRVRVISTPQGGGFGGKTDPFNHEIVVCKAAMMTGRPVKICLSREEV
                     FYCHRGRHPVLMKLRCGVKNDGTLTALHLKTLLDGGAYGSYGVASTFYTGALQTVTYH
                     LPNYRFDGCRVFTNKPPCGPKRGHGTVQPRFGQEIQLDKIAHALKLDPAELRLKIVEK
                     PNSLTANFLQVRTIGLADCIRSVVRRSGFSDKRGKLPAGRGVGIACSSYLSGAGMPIY
                     YNDMPHAGVQIKIDRSGLVTAFCGAAEVGQGSDDVLCACVAEVLGVEVADVKVVTGDS
                     DLTPVDLGSYSSRVTLMMGNAAIAAAERAREIIATPVAEKLGIAAEQLVFSHGRVFDA
                     AAPEKGMSFREAVIVAESAQGTIGATGSYKPQKPAALFKGGGVGPSPTYSYSAAVVEV
                     EVDTETGWLHVDKIWIAHDIGRALNPTLARGQVEGSVYMGLGEALMEEQAFRRLPPKL
                     SSALVHRTPSILEYKSITSLEMPDVDIELIEDPDPNGPFGGKEVGQGPLLPIMPAVAN
                     AIFDAVGVRIDEVPITPEKILRALKLKAEGKPARIGPKDFPSVPYPEPLQVPTPWQGG
                     DGRATNDVVRDKSKRAKNV"
     gene            8951..9976
                     /locus_tag="PKL17_07960"
     CDS             8951..9976
                     /locus_tag="PKL17_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNK44701.1"
                     /translation="MLRLPEFHLLAPQSLSEALAMLAADPQNTSVLAGGTDLLPNMKR
                     RQQTPARLLSLRNLTALRHIHVSDGEIRIGGAVRLREIEDHPLLQAEARALTQAVSQI
                     ATVHIRGTATIGGNLCLDTRCDYYDQSEPWRKAIGYCKKKDGDICWVATSSPRCLAVS
                     STDAAPALMALSARVVLSSQNGDRELDLADLYHNDGMAYLTKRPDELLTEIRIPRLPS
                     RKSAYWKLRRRGSFDFPLVSVAAAVQLGSDGKVAQAGLALGAVSSRPFLVTDAAQLLV
                     GQPLSDDRIAQVADAAHALAKPMDHADMSLSYRKTMVKSLTRSALCEVRGDDVTKERL
                     RLMRLIG"
     gene            complement(9988..11286)
                     /locus_tag="PKL17_07965"
     CDS             complement(9988..11286)
                     /locus_tag="PKL17_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNK44702.1"
                     /translation="MHLSRREAIVSALFGAGSLGLRALATGLPASLLLHPHKSWADAC
                     ANRDAAQYLIWATSGSGDPSNANVPGTYDDPGIAHPLDAAMAPTPMTLSGKTYTAARP
                     WATLPQALLDQTCFFHHTTLTNSHANEGKVLKLMGAVKRQEMLVSLISKHLSPCLSTV
                     QPEPASVSGEVITFGGRILPALTPVGLRDVFVSPKGPLTNLQALRDADLDRIHAVLKQ
                     NGNSVQRSYLDRMAKSKSELRNISDSLLGSLATIKANDLDGQLTAAVALIQMNVTPAS
                     VIFIPFGGDNHSDANLARESAQHVSSCAAIASLWQKLAAAKLDQKTTFVLMNVFGRTL
                     NRPQRMGRDHLGSHHCTVISGKRVRGSVVGGVMKSGSDYAATPIDSKTGLAAMGTAAD
                     IRFEDTLGAVGKTIAAAVGLPNDVLNDQITTGKVVTAALT"
     gene            complement(11298..13277)
                     /locus_tag="PKL17_07970"
     CDS             complement(11298..13277)
                     /locus_tag="PKL17_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HNK44703.1"
                     /translation="MRRAQGTLLGMFALGMALSAISCEQAPATMGPESVAPVENTELP
                     ATFEAMGPYSYVGKVKNVLTGLPPSDAELLAVQKDETALRGLIDDWMKTPQFRTRMLA
                     FFATAFQQGQLNRASLLDMVPDAPIGDRMLGNIMASFPLTVWQLTMVEGKPLTEALTT
                     ERFMMTPPLATLLAFLDNRYTTDANRGTDYITTANPNFKITLSNKSGPIAIEKTLDPQ
                     DPLFMNWYLPVAVPAACTDPLVYPRDTPRLYQFLFGSVNASTGVCTQSFAVPSQFADA
                     EYDAWRMVQIRQPKAGEASSLFYDLTAMRNTGELVLRVPRVGFFTTPAFFANWATNAS
                     NQARVTINQTLIVALGRSFDDSNNTLPLALPAMDKAHSSDPACYPCHKTLDPMRQVFR
                     QAYTLTYHEQKDPAQTSMPGVFAFDGISKNVSTPKELGQAMAGHPRFAVAWAQKLCYF
                     ANSAACSESDPEFQRIVMAFQSSGHDLKTLLREVLTSPLTTGTRNTQTFADQGMTLSV
                     ARRDQLCGAMSTRLGVPNLCALGRAPSLAQLIPSDGFSRGGTAPILATDPSLFYRAAT
                     EQLCRNFADYVVDSNVTGKPSRYQSKLPDAAISDMVQTVMSVVPSDPRYGSLLAILSE
                     HYANASKTQGITGVDALKSTFTLACMSPTSLSLGL"
     gene            13416..14219
                     /locus_tag="PKL17_07975"
     CDS             13416..14219
                     /locus_tag="PKL17_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44704.1"
                     /translation="MWSDRRGTAKASSGPHRRWLVAALVLGGLVTDVSCGPQIPSGQP
                     LTEGDLAVAPIDWNVAPLAAGKVVAIAENDDDVALFSDGSVSVWSGGESLGRDSSVRA
                     WRSAAAVPALGFSGRWLLGVSDDGKLYRLHYGEAQTLSCEDVSARYLLSNSPVREVAA
                     LGGALVGFVTDGRLAISDGQRLKQYELSLHGLTGSLGKAAGITDDEVVVLFAQDDTLQ
                     RLSLPSVNWVAFDGAGMLWAASGSGLYRQRDDHFEQVLSPADGQSIRGL"
     gene            14247..14669
                     /locus_tag="PKL17_07980"
     CDS             14247..14669
                     /locus_tag="PKL17_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNK44705.1"
                     /translation="MSDGVALVRDGQLLRAPLPTVGNPGDDAAAMLRVLGSPSGDAWL
                     LTDSQVVRVGEESGGGQDLVLWRKQMQPVFVRTCQGCHLPSGSAHLDLSTHSSWARYR
                     RVLAQRVVAGTPTPMPPTGAGKLTADELAQVMAWTSRP"
     gene            14875..15858
                     /locus_tag="PKL17_07985"
     CDS             14875..15858
                     /locus_tag="PKL17_07985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK44706.1"
                     /translation="MRVSIAVPAYNEERNIGELLDTLRAQRTKRAKIVEVVVVASGCT
                     DRTADIVRERAARPGVPVRLIEEPERRGKVAAINAYLRVLNPHVDAICVCSADLLVSR
                     EVVERLVQCFLINPEVGMCGGRPVPTNRIDSFTGAATRFLWQMHHRVALEAPKLGELV
                     MIRAGIVRNLPPESAVDEASIEQLVCNAGYRLAYVPEAVVHNHGPETVRDFIRQRRRI
                     AAGHLWLRSVSGYSVSTMNVWRIARLALAEIDVSHPREALYRLGTIGVEAVSRVLGFV
                     DFHTNSTKHAVWKVSESTKRVMNDEVRGLYLSDSVDDAPLARPESGRATGT"
     gene            complement(15872..16630)
                     /locus_tag="PKL17_07990"
     CDS             complement(15872..16630)
                     /locus_tag="PKL17_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNK44707.1"
                     /translation="MIRRSPFLLLGICLPSLAVGNPLAQSQQLLVVLSDGFDAPRGTL
                     YRFERRQSSSSLVRVGSPLPVWLGRSGLGWRSDPGAPIPPVEGPVKREGDGRSPAGIL
                     PIGEMWGYAPAAPGGVKLPYHQATAQDRCVDDVASPSYNQIVRESAAPSWKSAEHMKM
                     PTDHYKYLVVLGYNLPKARPGAGSCIFLHVAPPPQGGTAGCTALFEKDLVDVLRWLDP
                     KRTPVIVQLPKQVLTSAIKAWQLPTELAQLANTP"
     gene            complement(16623..17237)
                     /gene="ruvA"
                     /locus_tag="PKL17_07995"
     CDS             complement(16623..17237)
                     /gene="ruvA"
                     /locus_tag="PKL17_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNK44708.1"
                     /translation="MIARLTGILADKTLDSVLLDVGGVGYHVSVSLCTLATLPTVGSK
                     VELFTHLHVREDAMQLFGFATRDERKAFELCTSVSGIGPKLAIGLLSGLDAAGLFAAV
                     AAEDVARLRKVPGIGPKTAERLVLELRDKVPRPEGRSKPQPRTKQDPAAVHVGLFADV
                     MGALVNLGYRPTDAERATEQAIKAQPEAQIETVVRTALRALQRD"
     gene            complement(17305..18669)
                     /locus_tag="PKL17_08000"
     CDS             complement(17305..18669)
                     /locus_tag="PKL17_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNK44709.1"
                     /translation="MSHFAYRPGMPLCGLLSVLSLGSPACSPPDPCQGQSSSCLTVQV
                     ETQGDVKIDLIRTIYSVNGGTSQQQGFATASNTASSLPIAFALALGDGGTAQVDIVGE
                     LGANAVLRGSTTETLAAGEHKRVVVSLGRDVSKLPFIGPEPRYGAGLAAVPISAPTTI
                     VMFGGVVVGDQPLSETWEYDLTQNTWQRSTSAMSPGPRQPQMVADPTADRVLVVQGLG
                     AGGVVQPDAWQFGPTMPGAVRSWTPLTTVTSPLPPRGAAGLTILPSMTGTPLAFFFGG
                     IDGVGGNPRSDLWRMLTPSMTNFATTAANGAPVIRGPKLFSTQTDVYLVGCEDTMQTV
                     MKVWRLDTTTTPNNWMVLSNDGGAPSYRQSFAAAIDPQTGMIAVFGGKAQDGSFLSDS
                     YRFNVQGGSWSTVSGDQNPPARIDASMTFANGRYYLVGGRDSNLKPRLDNWKLTTEGW
                     KRWL"
     gene            complement(18666..19265)
                     /locus_tag="PKL17_08005"
     CDS             complement(18666..19265)
                     /locus_tag="PKL17_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44710.1"
                     /translation="MEKVIARSLLLWGIVGLASPVVHAAEPPTAAAKAAAKKAYEQGT
                     AHYKKGEWDPAIEQFELCYKNLPQPVFLFNIAQSHNKAGRPEKALEYYKKYLNDAPTA
                     PNRAEVEQTVAELEQQLTPAKPAVEIAQLQTFPVEVASEPPPKGFVAKSSIWPFSKKT
                     WIIIGAAAGGAVVLGTAIGLGVYFGTRGPTVTVFNPVNP"
     gene            19312..20223
                     /locus_tag="PKL17_08010"
     CDS             19312..20223
                     /locus_tag="PKL17_08010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNK44711.1"
                     /translation="MQTERGGLQVGAVTEVVLLFVVGLLLLVVIGVLWGGMSGPLLSW
                     LGLRALSTGRWAVIPQVVLPELLGLCLPACLFLKWRQGSPACQDVPLDGSSLGRLLQQ
                     LVAGLLLGGSLFYFLGVWLLPHYERLVPLSPSEQRALLRMLVPPQGLRPLWIDLLTFA
                     LCPALCEELFFRGAILRHLLAARADLPAEMAAWGMQKRATAVVLSSLLFGIIHLSWGR
                     LVPTAVLGAAFALAVLRSGSLWTVIAMHFANNAVVVMLARQGISSLTSVPMATSGALL
                     VPACLALGLGIWLLKPRSTRIDSRCSA"
     gene            20257..21444
                     /locus_tag="PKL17_08015"
     CDS             20257..21444
                     /locus_tag="PKL17_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44712.1"
                     /translation="MADLFLRRSAFCARVLLGFFWLGLPLRVSMAQKPVATQSPLWVL
                     PVSSPPTLPGLGKELQRALLGALKEANLNAQPGKSDSDGGISARLEELGTGRFRLTLS
                     HRSGSSQSVGDLEHLDDLVYAVVAELRPKLHSELLPSDPSQIPSLGASKLAALPSPQV
                     VSKQSPTIQTAQFGLGSKEIKLATALLGSSAKSKREPQRTESVVPAQLPPPTPVAVSN
                     SATAPTPPVTSNLPPASTTTNPTTPASAQTDPEQKPRTRPRIAVGVMGEPGSLPPGYF
                     GLGLTGQQALLAFLQQRLQVPAVATRLYSLVGGFEALEQSLRVSARHTLMARLDSLSV
                     TSGIMSGRIHLVLLHDGKLLYDRSVALPPTAVSAAEPPATAFGRAVTSAVESLSNDLL
                     TRL"
     gene            21624..24377
                     /locus_tag="PKL17_08020"
     CDS             21624..24377
                     /locus_tag="PKL17_08020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44713.1"
                     /translation="MALPSPFGKYLLLERISVGGMAEVFKAKTFGVEGFEKIIAIKKI
                     LPAMAEDADFIQMFIDEAKIAGQLNHANICQIFELGKIDDSHFIAMEYIWGKDLLQIQ
                     NRFRRLKKHMPVQMSAFVMGKVCEGLDYAHRKKDATGKPLNIIHRDVSPQNVITSYDG
                     EVKVIDFGIAKAASRSSKTQAGVLKGKFGYMSPEQVGGKPLDQRTDIFAVGTILYELL
                     TSERLFMGESDFATLEKIRNATVPPPSTINKEIPPELDRIILKALARDPNERYQWGSE
                     MHDDLADFAAQFEPVYNAKALATWMREAFSPEMKKESAVLEQQRKIGKEAMTQGGQAK
                     GGAPPSARLRSPTLAPTSSAPRAAPTESPDPTSALSEAEIEPLAEGEGGGEGGDLLNQ
                     ATAIVMPDQLPNAGEGDLPNQSTAIFDSAKHGGGEDENDIKAQSTAIFDPSSHKGAQP
                     AQPAPAPAPPQPQMDPFAGMNPGYPQNPSMVGPMGAFPSAYGSAVMPAMAMPFATGSM
                     SALPAGAMVMLPNGTVIPASAVQQPAPPPPPVAPVPEQRGGSLGKDILVGVLVAIAVV
                     GLVLGGKFALQPGKGTVVVTVFPPRPATVYIDGREAGKVEQGGALLLRDVGKGQRQLL
                     VRAEEGESQQVIDVLAGDTVQVTAQLGAGSAAAQTSGQGNGVIRLRVPPGVFISVNGV
                     LVKESDILKGYLVPAGMVQNVSVKKAGKQPVNFQVTLQSGQEYDRAIELQEGRGKLVV
                     NTKPAGAEVLVNGRSYGKSPVTVEDLDPTKTAKVVLKKRGHGSVTKLVSFGDAFEQSL
                     DIDFATGGKDDDKGSSKSSGSSDSAQASSATPAKTETPAAASSSGDKEGFLIANSQPY
                     ARVFVDDKDTGKTTPIAPRDRIPLKAGKHKVTFVTSGKRVNFDVVIRAGEEYKLVRNL
                     NED"
     gene            complement(24451..>25655)
                     /locus_tag="PKL17_08025"
     CDS             complement(24451..>25655)
                     /locus_tag="PKL17_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNK44714.1"
                     /translation="LWALISRLVSDGVDELLPFLIGDYAKDLVFSTLLGVLTTNPRRQ
                     RVIYATFVLAMLFAAIVSIPQRFGPKQCHYFRFASQLNYEQTSDRRPCQSTAECYTVP
                     QHEQHLRDFGWACERTGPLGLATVVDRIHYTGNLLDPNALALALVMAAALLVGLATWP
                     DPSPRRWLWLIGLPVLGLAVILAASRAAQVAMGAVLLCFFYFRVGISGAVLAAVLSSP
                     LLLISTRNEAEAAYSTVTRIMTYLNGFRAFLDYPLFGVGFANYERISFLNAHNSFLQA
                     LVETGIIGGALYLTGIYASAKLLIQVIQFPSEELLATEPARLEEIQHIAKTLLAMLLG
                     VLLCVLFLSLAFDFSWLVPLGLVAGFELSLRDEFPTHRLRIGIIELVAMIIGTLLFLG
                     VFVLVTAR"
BASE COUNT         4844 a         7513 c         8254 g         5044 t
ORIGIN      
        1 cgtcacgctg gcagtcgcgg cttttgcgcg gcaaaaagcg ggcgtatctg catccagcgg
       61 gaaaagaggc ggtgacgctt gtgcagccgc tgtggcgcga cggcgatcat gtgctctggc
      121 agctgtcgcc gctcaccgga cggcctcatc agctgcgcgt cgagctggcg cggcgtggct
      181 gtccgattct cggcgatgcg ctgtatggtt cgcgcgtgcc gtgggcggac ggaattgcgc
      241 tgtgtgccgt cgaactggat ttttcggcgc tgcccgatca gtcggaactg gggctgccac
      301 cgtcgctgcg cttgggcggt gatgacgcgg ttcgcagcgg catcgcgtgg cgcaaagcgt
      361 tcgccagcaa gtccggacat tcaatccaca gcgcgtgtcc caccgacgga atcagaaccg
      421 gggccggctg accgacgagc ttcgccaagt tggcttgctg cgtcggtggc atcagaaaat
      481 cctcactccc cgcaatcacg gcgcaagtct ttgcagcatc cggcgggcag ccggggccaa
      541 agcgatgcgc aagctgtcgc gacagatcgt gcgcgatgag cgcttgtagc tgtccttgca
      601 cggcgtgctc gcggtggctt tgtccgtcgg atagcgcatc gagcatcggc aaaatgctcg
      661 gtggttctag aaaccgggct gaaaagcaca gcacatgcag cgtccgcaga agcgtcgccc
      721 gcggcacgtc ggatctgtgc agcgccagca ggctttcgac aatcgctcgc agccgcggat
      781 cgaccgtcgc tgtcgtggca accagcgcga cgtgtcggaa ctgctgcggg gcggagacga
      841 gcgcatgttg cgcccacatt ccacccatcg aaaaaccggc gcagtcgacc tgcggcaagg
      901 acagcgtcga aagcagggtt agcaggcgtt cccgctgcgc cgaaaaatca taagctgccg
      961 cttcggtggg ggcttcgctg cgtccgctgc cgagtggatc gaccaaaagg acgcttcgcc
     1021 gcaacagccg agccagccgc tgaagctgcc gcccatcgga caaaagacct ggcaaaagca
     1081 aaactggcgt cgaatcgggg cagtcgtcgg gcaccgccgt gagctgaaaa aaaagcccgg
     1141 caatctggcc cgtgatgggt ttttctcgtt tcagcaaagc aacttacgat acgccgtgac
     1201 ctggcctgca caagctagac tgatggtgat gcgacggttg attgtcggac ttgggctgtg
     1261 tgggctgctt gcgcttggtg gggcgacggg ctgtgggccg cgggcgattg tttccgacct
     1321 gacacaccac aacatgtcgg acgaggattt gctccagctc ctgccgcagg gacaagaggc
     1381 ggttttcgat atcgagcttc agtcgctgcg ggcttggccg cctgcgcgat cgctaatggc
     1441 actgctgccc gaaggtgcgc agcgacgatt gcgagacgtg gctgccgatc cgctcagcga
     1501 tctggacgcg gtctgcatcg ggctgcgggg gcttggcaca aaagacgtcg aagcgacgct
     1561 gctgtttcgt ggttcaccgt cgcgtaacaa gctgtggcag cagatgctga gccggcccca
     1621 aagtcgcgaa gtcacctatc acggtatgcc catcgccgaa gtcgccgacg agtcgctcgc
     1681 gcagcttacg ccccagacgg tggtgctggg ttctcgactg caagtgcgac agaccatcga
     1741 catttttcgg ggcgtcgatc atggggcgcg caaacagccg gcgcttgtgg cagcgcttgc
     1801 gatggcaccg acggggaaag ctggacgacc agcggtgttg atggcgattt tgccgtcgtc
     1861 ggatctgcgt caaaaagcgc acaatgccgg cctgctgcgg atcttggatg atgcgcaagc
     1921 ggtggcggtg gcgatggcga ttggcgacgg cttcgacatt gggattgtcg cgtcgtatcc
     1981 cgatgtgacg atggctcaat cggcgctggc gtttgtgaac gcgcaggcgg cggagctgtc
     2041 tttgcggcca cttaccaaag cgcttgggct tgaacggttt atctcaccgc tcgtcgctgt
     2101 cgcggtgccg cagtcgaaaa aaaggccgac gccagagctg caccttgcgt atcgtttgcc
     2161 cggtgatgac ttggcggagc ttctgcgccg gatcgaacag ctaaaacagc tttttccgtc
     2221 acagcgtaac tgagctgcct tcgcgcgaag cgataagctc gatgtggcga ccgccgagcg
     2281 agtcgagcag ctcgcgacag cgcgaataga tcgaatccac cgcgtcgtcg ctgtagtcct
     2341 ggtcaaagtg gaaaagggcc aggcggcgaa ccccagcgcg cactgccgca tacgccgcat
     2401 cggcaaacga cgaatagcca cgggaccgac ggaccgatcg atcttcgggc gtatacgtcg
     2461 agtcgtgaat cagcagatcg gcgccttgat agagccgcaa cacttcttcg cttgggcctg
     2521 catcgggata accagcgtcg ggggcataga ccagcgatcg gccattttcc tcgatccggt
     2581 atgccatgca cgtcgacgga ccgtgtggat tgggtcttcc tgatatcgcg atgccttccc
     2641 aaaagatcgg cggcggatcg ttgtccgtcg gaacaggcaa aagctcgaac atcgcgccga
     2701 ggttcttcat cgtctgaagc ggcgagaagt gcggattgac ctgaccttcg aggattcctt
     2761 ccagcatcgc ggcgctcttg gccggtccga agacggaaaa gcgattgccg gggatgaaca
     2821 cgggcgcaaa aaacgggaag ccttggatgt gatcccagtg ggcgtgggac agcaggatgg
     2881 caccgtggcc tttgcctttg ccaaactcgc tgccgagcag ctttcgcccg aggctgatga
     2941 tgccggtccc catgtccaaa attaggatct tgcccgattc cgatcgcacg ctgacacagc
     3001 aggtgttgcc gccaaagcgg actgtgtcgg gaccaggggc gggaatcgac ccgcgtacac
     3061 cgtgaaatgt tacttccacg actcttcctc gtttgaactg gagccgcgat ggccctgacc
     3121 catcgatcgg ctgctggcat ggcccacctg catgccgtgt ttgcccaggt cgacttccat
     3181 tccctcgtaa gccgcgtgca gttccagtcg tgcaccgtcg cgcgcaagct gatgccgatg
     3241 ctgaccaagc agctcgtcga tctgcgcatc cgagcgatcg ggcgagtggt gatacagcat
     3301 cagtgattct gcaccggcct cgcgggcaat ttcgatcgca tcttcggcgc agctatgacc
     3361 ccagtgcggg cgctgcgcgt attccgtcgg cgtaaactgc gtatcgtaga tgacgagctg
     3421 agccccttcg cacagccgga ccacgccttc gcgcatcgcc gccagcttgg ctgcatcttc
     3481 gggcgcaagc ggctcgccgg gcttgggcgg ctgacggatg aactcgtgtt ctatcaagat
     3541 gtcgcggaac ggcgccgtgt cggtcacgta agcgacggaa gcgccttcat aatcgagccg
     3601 gaacgcgatc gcgatccacg ggtgattcag ccgcgtgcag cgcactttca ccgggccaat
     3661 ttcaaagcgt gcatcgtcgg tcagctcgga aaactgcaca ttggccgccg ccgcgtcaaa
     3721 cggcaccgga aagtacggat tgtccgattg aagtgcgaac accatccgca ggtgcatgtc
     3781 atcgcgctga cgggcgtaga catggaattt gttgccggga cgatacagcg gcgaaaaaaa
     3841 cggcaggcct tggatgtgat cccagtgggt gtggctgacg agcaagtgcg catcgccttt
     3901 gccctcgccg aacggcccgt gcatcagatc cttgccgagc cgccgcaggc ccgttccggc
     3961 atcaaggatg atccggggcg cgctggggtc ttgcgccgaa gcgcagcgaa cttcgacaca
     4021 gctggtgttg ccaccgtagc gctgcgtcgt cgtgccgggc accggatagg agccgcgaac
     4081 gccccagaac cttagcttca cctcggcccc ctttttgctg atggcatgcg ctcatcctca
     4141 caacgtcggc ctgacgcatg gaggagatag gacgccctgc tcatcatgag atgctttagg
     4201 ggtttcatag cttgctaaaa acagcttcca tcgtaacacg cggggttttc agaaatctac
     4261 agaatctccg ccgggtcgcg ctgcgatggg ctcctgtctg gctgtaggcg ctttgtctgt
     4321 cgcggcgggg tctgtcgcgg cagggtctgt ggtgggtgcg cacggtggct tctgtctcct
     4381 tgggcttggg tgggaaagcg tgctacaagg cgacccgata tgcggaacat cggcgtgcga
     4441 cttggctttg tggctctgct cctgctgggc ggctgtaccg tcgtccgtcc gtatcagcgc
     4501 gaggcgttgg ccgagcggtg catggcaccg ggttttggcg acaagtcgga agtgaagttc
     4561 cgcgcccact gggaatacgc acggcatggc acccaaggcg gctttggcga cgatgcgggc
     4621 ggcggagggt gcggttgcaa ctgaccatgc caaaaccagg tgctgggcgc ggcttggctg
     4681 gcgggcgctg ggccgcgcgg ctgtgctttt tgtgctttgt gctgtggacg ggcggactgc
     4741 gagcggaaca gctgccgcaa aacgtcagtg gcgaagcggc ttttcggggc agctactggc
     4801 gcgatcgcaa cacgcgggtg ctcaatccga cggtcgatct gcgccaacag acatcaagcg
     4861 gcgtatcggt cactggtgcg tatggcctgg atgcgatcac gtctgcgtcg gtggcagctg
     4921 gggcgacgac cgatcagcca tttaccgaac tgcgccacga aatcggtttc ggactcgaag
     4981 ttcccctggc tggcaaatcg acgctttcgg gcgggtacgg ctattcgacc gagagcgact
     5041 atttttcgca tactgcgtcg ctgcggacca aaatcagcct gctgcaagac aacaccattt
     5101 tgcgcatcgg gggcgactac acacactcgt cggtgggcaa gcggctcggg ccgaccggct
     5161 acttgaacat gggcattttg cacgaagccc acgccatcgt cggtgtttcg caagtgatga
     5221 gtcggacgat tgttggctcg ctcagctacg agctgtttgt gctgtcgggc taccaaaaca
     5281 atccgtatcg tccggttttc gtcgcgggcg agcggcgcga gtttgaaaat ctgccgcggc
     5341 tgcggattcg ccacgcaatt ggtatgtcag gtcacaaaat gtttcgtttt gaccggacat
     5401 tggtgccgca cctgacgctg cgaccggcgc tgcgcatctt ggtcgattcg tgggggacaa
     5461 agagtgtcag ccccgagctt gcggttcatt tgccgatagg ccccgtcgag ctgcgcggtc
     5521 ttctgtcgta ttttcatcag gccgaagcgt cgttttatcg ctcaagctgc ggcgagcagc
     5581 ccaaggtctt tccgggggcg ccctgctatg ccgaacgagg cgtcgaattt ggcaccgcca
     5641 ccgatagcaa cggcaacgag acgacgttta tggcctatac ctcggacccc aagctgggga
     5701 cgtactcgac gtggaccacc gagctacaga tcaagtggcg gatgacggtg ttttcccact
     5761 tgggctccgt cggttcgctt ctgtctcgca gcgccatcga gctgtccggt ggaatgtggt
     5821 ttgcgcagga cgcgattggc aaccagtttg gcattccgct ggtcggcaac gaccaagttg
     5881 gcgtcgccgg ttgttccgct ctctgtggcg ccggttttgc ccatctgggt ctatacgttc
     5941 cgctgtaacc atcgcgaaga gaaaccaacc aatccgacaa ggagtgggtt tgtgaaacag
     6001 ccgattaccc tcgtcgtcaa cggcgaaaaa cacgacacgt tgtgtacgcc caacctgacg
     6061 ctacttgagg tactgcgtga aaaccttcag cttaccggca ccaagcacgg ctgcgagctg
     6121 ggtgagtgcg gcgcttgtgc agtgcttctc gatggagaac ccgtgctttc ttgtctggtc
     6181 ctggcgctgg aatgcgaaag ccgcgcgatc acaacggtcg agggactaaa gcgcggtccg
     6241 gttctgcatc cgctgcaatc ggcgtttgct gatcacgggg cggcgcagtg cggctactgc
     6301 acgccgggga ttttggtgac ggccaaggcg ctgctcgaca aaaatccctg tcccagtcgg
     6361 tccgatgtca aagaggcgct gtcgggcaat ctctgtcgct gcaccggcta tctgcaaatt
     6421 ctggaagcgg tcgatgccgc ggcccaggtg ctgcgcgagg aagcttcgtc acaagggggc
     6481 gctgcgcgat gagccatcgg cctctgcaca tcattggaaa accccgccgt cgcgtcgacg
     6541 gtcgcgccaa agtgagcggt gaaacccgct atgccgacga catctcgctt ccgcgcatgc
     6601 tccactgtcg gctgttgcgg tcgccgctgc cgcatgcccg gattgtgtcg attgatgcca
     6661 gtcgcgcgct ttcgcagccc ggcgtgcatt tggtcctgac cggccaagac atgccgacga
     6721 cgttcgggat tttgcctgtt agccaggacg aacacgcgct gtgtctgcac aaagtccgct
     6781 tcgtcggtga tccagtcgcg gcagtggtgg catcgagcga gcttgtagcc gaagaagcgc
     6841 tgtcgctcat cgacgtgcag tatgaaccgc tgccgacgat tgcgtcgccc gaagaagcgc
     6901 tggccaccga agatccgcgg attcacgaat acggcgatca gggcaacatt cacaaagcgg
     6961 ttgcgatgtc gttcggcagc gtcgatgacg gctttgcgca ggccgatcac atcttcgagg
     7021 acacgttcca ttacgccggc aacacccacc tgccgctcga aacccatgcg gcgctggcgg
     7081 cgcttgatcc cgacggcaaa ctgacgattt ggtcgagcac gcagacgccc cactatgcgc
     7141 accgcgcgat tgccaaagtg ctggggctgc caccgtcgcg ggtgcgggtg atttcaacgc
     7201 cacaaggcgg tggttttggt ggcaagaccg atccgtttaa ccacgaaatc gtcgtctgca
     7261 aagccgcgat gatgacgggc cgcccggtga agatttgtct gtcgcgcgaa gaagtcttct
     7321 attgccatcg cggtcgtcac ccggtgctga tgaagctgcg ctgcggtgtg aaaaacgacg
     7381 ggacgctgac ggcgctgcat ttgaagacgc tgctcgacgg tggtgcgtat ggttcgtatg
     7441 gcgttgccag cacgttttac accggcgcgc ttcagacggt gacgtatcac ctgccaaact
     7501 atcgtttcga cggctgccgg gtgtttacca acaagccgcc atgcggaccc aagcgcggtc
     7561 acggcacggt gcagccgcgg tttggtcagg aaatccagct cgacaagatt gcgcacgcgc
     7621 tgaagctcga tccggcagag ctgcggctga agatcgtcga aaagcccaac tcgctgaccg
     7681 ccaatttttt gcaagtgcgg acgattgggc tcgctgactg cattcgttcg gtggtgcgac
     7741 gttcgggttt ttccgacaag cgaggcaagc taccggcagg tcgtggcgtc ggcatcgcgt
     7801 gctcgtcgta tctgtcgggc gccggcatgc cgatttatta caacgacatg ccgcatgcgg
     7861 gcgtgcagat caaaatcgat cgttcgggtt tggtgacggc gttctgtggc gccgccgaag
     7921 tcgggcaggg cagtgacgat gtgctgtgcg cctgcgtcgc cgaagtgctg ggcgtcgaag
     7981 tggccgatgt caaagtcgtg actggcgaca gcgacctaac tcccgtcgac ctgggttcct
     8041 attcgagtcg cgtcacgctg atgatgggca atgcggcgat tgcagcggcg gaacgcgccc
     8101 gcgagatcat tgcgacaccc gtcgctgaaa agctgggaat tgctgccgaa cagctcgtct
     8161 tttcacacgg tcgggtgttc gatgcagccg caccggaaaa agggatgtca ttccgcgaag
     8221 ccgtcatcgt ggcggagtcc gcgcagggga cgattggcgc gacgggatcg tacaaaccgc
     8281 aaaaaccggc agcgctgttc aaaggcggtg gcgtcggtcc ctcgccgacg tattcgtatt
     8341 ccgctgccgt cgtcgaagtc gaggtcgaca ccgaaacggg ctggctgcac gtcgacaaaa
     8401 tctggatagc gcacgacatc ggacgggcgc tcaatccgac gctggcgcgt ggtcaggtgg
     8461 aaggttcggt gtacatgggg ctcggcgaag cgctgatgga ggaacaggcg tttcgtcgtc
     8521 tgccaccgaa gctgtcgagc gcgcttgtgc atcgcacgcc gtcgattttg gaatacaaga
     8581 gcatcacgtc gctggaaatg ccggacgtcg acatcgagct tatcgaagac cccgatccga
     8641 atggtccgtt tggcggcaaa gaggtcgggc aaggaccgct tttgccgatt atgcctgccg
     8701 tcgccaacgc gatttttgat gccgtcgggg tgcgcattga tgaagtgccg atcacgcccg
     8761 aaaaaatcct gcgcgcgctc aagctcaagg cggaaggcaa gccggcgcgg attgggccca
     8821 aggattttcc gtctgtgccg tatcccgaac cactgcaagt gccgacgcca tggcaaggcg
     8881 gcgatggccg ggccacaaac gacgtggtgc gcgacaaaag caagcgcgcc aagaacgtct
     8941 agggggacac atgctgaggc ttcctgaatt tcatctcctg gctccgcagt cgctgtccga
     9001 agcgctggcc atgctcgcgg cggacccgca aaacacatcg gtgctggcgg gcggaaccga
     9061 cctgttgccc aacatgaaaa ggcggcaaca gacaccggcc cggctgctga gcttgcgcaa
     9121 cctgactgcg ctgcggcata tccacgtatc cgacggggaa atccgcatag gtggcgcggt
     9181 gcgactgcgt gaaatcgaag atcatccgct tctgcaagcc gaggcgcgcg cgctgaccca
     9241 agcggtaagc cagattgcga ccgtgcacat ccgtggcact gcgacgattg gcggcaacct
     9301 ctgcctggat acgcgctgcg attactacga ccagagcgag ccgtggcgca aagccattgg
     9361 atactgcaaa aagaaagacg gagacatctg ctgggttgcc acgtcgagcc cgcgctgtct
     9421 ggcggtttcg tcgacagatg cggctccggc gctgatggcg ctttcggcgc gggtggtttt
     9481 gtcgtcgcaa aacggcgatc gggaactgga tcttgccgac ctgtatcaca acgacggcat
     9541 ggcgtatttg accaaacgtc ccgacgaact gctgacggaa attcggattc cgcgtctgcc
     9601 ttcgcgcaag agcgcgtact ggaaactgcg acggcgtggc tcgtttgatt ttccgctggt
     9661 ttctgtggcc gctgcggttc agcttggcag tgatggcaag gttgcacagg ccgggcttgc
     9721 gcttggcgcg gtgtcatcgc gaccattttt ggtgacggac gctgcgcagc ttcttgtcgg
     9781 gcagccgctt tccgatgatc gaatcgcgca agttgccgac gctgcgcatg cgctggcaaa
     9841 gccgatggat cacgcggaca tgtcgctgtc gtatcgcaag acgatggtga aaagtctgac
     9901 ccggtccgcg ctctgcgaag tgcgcggcga cgatgtgacc aaagagaggc tgcggctgat
     9961 gcggctgatt ggctagcgca ggcggactta ggtgagcgcg gcggtgacga ccttgccggt
    10021 cgtgatttga tcgttgagga cgtcgtttgg cagtccgact gctgcggcga tggttttgcc
    10081 gacggcaccg agcgtgtcct cgaagcggat gtcggcagcc gtacccatgg cagccaagcc
    10141 ggtcttggaa tcaatcggcg tcgcggcata gtcgctgcct gacttcatga cgccgcctac
    10201 gactgaaccg cgcacgcgct tgccgctgat caccgtgcag tggtgactac ccaggtgatc
    10261 gcgtcccatg cgctgcggac ggttgagcgt gcgaccaaac acattcatca gcacaaatgt
    10321 cgttttctga tcgagcttgg ccgccgcgag cttttgccac agcgacgcaa tcgccgcgca
    10381 actgctgacg tgctgggccg attctcgcgc caggtttgcg tccgagtggt tgtcaccgcc
    10441 aaacgggatg aaaatgaccg acgctggcgt gacgttcatt tggataagcg ccaccgctgc
    10501 cgttagctgt ccgtccaggt cgttggcttt gatcgtcgcc aagctgccaa gcaagctgtc
    10561 cgatatgttg cgcagctcgg acttcgactt ggccatgcgg tccaggtaac tgcgctggac
    10621 gctgttgccg ttttgtttga gcacggcatg gatgcgatcg agatcggcgt cgcgcagcgc
    10681 ttgcaggttg gtcagcggac ctttggggct gacaaacaca tcgcgcagac cgacgggcgt
    10741 gagcgcgggc aggatgcggc caccaaacgt gatgacttcg ccgctcaccg atgccggttc
    10801 aggctgcacc gtcgaaagac acggcgacag gtgcttggaa atcagcgaaa ccagcatttc
    10861 ttgccgcttc accgctccca tcagcttgag cactttgcct tcgttggcgt ggctgttggt
    10921 cagcgttgtg tgatggaaaa aacacgtctg gtcgagcagc gcttgcggca gcgtcgccca
    10981 cggtctggcc gcggtatagg tttttcccga cagcgtcatc ggcgtcgggg ccatcgccgc
    11041 gtcgagcggg tgggcaatcc ccggatcgtc gtaagtaccc ggcacattgg cattggacgg
    11101 atcgcccgaa ccggacgtcg cccagatgag atattgcgcc gcgtcacggt ttgcgcaggc
    11161 atcggcccac gacttgtgtg gatgcagcag cagagacgcc ggaagtcccg tcgccagcgc
    11221 ccgcagtccg agcgagccgg cgccaaacag cgcgcttaca atggcttcac gtcgagaaag
    11281 gtgcatggtg gaactcctta cagacccaaa gagaggctgg tcggcgacat gcaggccagc
    11341 gtgaaagtgg acttgagtgc gtcgaccccg gtgatgcctt gggtcttgct cgcgttggcg
    11401 tagtgctcgc tcaaaatcgc aagcagcgat ccgtagcgcg ggtccgatgg aacgacactc
    11461 atcaccgtct gcaccatgtc ggaaatcgcc gcgtcaggca gcttgctttg atatcggctc
    11521 ggcttgcccg tcacgttgct gtcgacgaca tagtcggcga agtttcggca caactgctcg
    11581 gtcgccgcgc gataaaacaa gctcgggtcg gtcgccagga tcggcgcggt tccgccacgc
    11641 gaaaatccgt cgctggggat cagctgcgca aggcttggtg ctcgtccgag cgcacacagg
    11701 tttggcactc caagtcttgt cgacatggcg ccgcacagct ggtcgcgtcg tgccaccgac
    11761 agcgtcatgc cctgatccgc aaatgtctgc gtgttgcgcg ttcccgtcgt cagcggtgac
    11821 gtcagcactt cgcgcagcag ggtctttagg tcatgaccgc tcgactgaaa tgccatgacg
    11881 atgcgctgga attcgggatc actttcgcta cacgccgccg agtttgcgaa atagcagagc
    11941 ttctgcgccc acgcgacggc aaagcgtgga tggcccgcca tcgcctgacc cagctctttg
    12001 ggcgtcgaga cgttcttgct gatcccgtcg aacgcaaaca cacccggcat cgacgtctgc
    12061 gccggatctt tttgttcgtg ataggtcagc gtgtacgcct gccggaacac ctgccgcatc
    12121 ggatcgagcg tcttgtgaca ggggtagcaa gccgggtccg atgagtgcgc tttgtccatc
    12181 gccggcagcg ccagcggcag cgtgttgttg ctgtcatcga agctgcgtcc gagcgcgacg
    12241 atcagcgtct ggtttatcgt cacccgcgct tggttgctgg cattggtcgc ccagttggca
    12301 aaaaaggccg gcgtcgtgaa aaagccaacc cgcggcaccc gcaagaccag ctcgccggtg
    12361 ttgcgcatcg ccgtcaggtc ataaaacagc gacgacgctt caccggcttt gggctgccgg
    12421 atctgcacca tccgccacgc atcatactcg gcatcggcaa actgcgacgg caccgcaaac
    12481 gactgcgtac agacgcccgt gctcgcattg accgaaccaa acaggaactg ataaagccgc
    12541 ggcgtgtcgc gcgggtagac cagcggatcg gtacacgctg cggggacagc gacgggcaga
    12601 taccagttca taaacagcgg atcttgcgga tcgagcgttt tttcgatcgc aatcggtcca
    12661 cttttgttcg acagcgtgat cttgaagttg ggattcgccg tcgtgatgta atcggtgccg
    12721 cggtttgcgt cggtcgtgta gcggttgtcg agaaaagcca gcagcgtcgc cagcggcggc
    12781 gtcatcatga accgctcagt cgtaagcgct tcggtgagcg gcttgccttc gaccatcgtc
    12841 agctgccaca ccgtcagcgg aaagctcgcc atgatgttgc cgagcatgcg gtcgccaatc
    12901 ggcgcatccg gcaccatgtc gagaaggctt gcgcgattga gctgcccttg ctgaaaggcc
    12961 gtcgcaaaaa acgcgagcat ccgagtgcgg aactgcggcg tcttcatcca atcgtcgata
    13021 agcccgcgca gcgccgtttc gtctttttgc accgccaaaa gctcggcgtc cgaaggggga
    13081 agccccgtca gcacgttctt cactttgccg acgtagctgt aaggacccat cgcttcaaaa
    13141 gtcgctggca gttcggtgtt ttcaaccggc gcgacggatt ccggtcccat cgtcgctggc
    13201 gcctgctcgc atgagatagc cgaaagcgcc atcccgagag caaacatgcc caacagcgtt
    13261 ccttgtgccc tgcgcattga ttggcctcct gacgtgttcg caaaacgtcg ccgcaaagcg
    13321 tgacgttaaa aaatagcggt gatgggccgc agattgggaa tctttttgcc gaatttttga
    13381 cgcaaaatcg cagcccaaaa cggccaggag acgccatgtg gagcgatcga cgtgggacag
    13441 cgaaagcgag cagtgggccg cacagacggt ggctcgtcgc cgcactcgtg ctgggtggtc
    13501 tggtgaccga tgtcagctgc ggtccgcaga tcccgtccgg tcagccgctg accgaaggcg
    13561 atctcgcggt ggcgccaatt gactggaatg ttgcaccgct tgcggcggga aaagtggtcg
    13621 cgattgccga aaatgacgac gacgtggcgc tgttttcaga tggcagcgtg agcgtgtggt
    13681 caggcggtga atcgctcgga cgggacagct cggtgcgtgc gtggcgatcg gcggcggcag
    13741 ttccggcgct cggtttttcg gggcgctggc tgctcggggt ttccgacgat ggcaagctgt
    13801 atcgcttgca ctatggtgaa gcccaaaccc tgtcttgcga agatgtctcg gcgcgctatc
    13861 tgctatcgaa cagtccggtg cgcgaagtgg cggcgctcgg cggtgcgctg gttggctttg
    13921 tgacagacgg gcgattggcg atttccgatg ggcagcggct gaaacaatat gaactttcgc
    13981 tgcacgggct gaccggcagt ttgggcaaag ccgcgggcat cacagacgat gaagtcgtgg
    14041 tgctttttgc ccaagatgac acgcttcaaa gactgagttt gccgtcggta aactgggttg
    14101 cgtttgatgg cgctggcatg ctgtgggccg cgagcggaag cggactttat cgtcagcgcg
    14161 acgaccactt cgaacaggta ctttcccctg ctgacgggca gtcgatccgt ggtctttagg
    14221 cgggatctgc tggtctgtgg ctgcttatgt ccgatggtgt tgcgctggtg cgcgacggac
    14281 agcttctgcg tgcgccgctg cccaccgtgg gcaatcccgg cgatgatgcg gcggcgatgc
    14341 tgcgggtttt gggctcaccg agcggagatg cctggctgct caccgattcg caggtggtgc
    14401 gcgtcgggga agagagcggt ggcggacaag acttggtgct gtggcgcaag cagatgcagc
    14461 cggtgtttgt gcggacctgt cagggctgtc acttgccaag cggcagcgcg catctcgatt
    14521 tgtcgacgca tagcagctgg gcacggtatc ggcgggtgct ggcccagcgt gtggtggcgg
    14581 ggacaccgac gccgatgccg ccgaccgggg cgggaaaact gaccgcggat gagctggcac
    14641 aggtgatggc ctggacgtcg cgtccgtgaa acatccgtga atgctgtgtg aatgagcagt
    14701 gaacgacgca gcgatttttt gaccccttgt ggcgcaaccg cttgaaattg cctagattgt
    14761 ggatgggaat gtcgcctttt cgccgtgctg gcttgctttc cgcccagtag gttgcgatag
    14821 attcccattt gcgagttttg cttctctata ccgcttagac taacgaggtt tcgtatgagg
    14881 gtgagtatcg ccgtccccgc ctataacgag gagcgcaaca tcggtgagct tctcgatacg
    14941 cttcgggccc aacggacaaa gcgtgccaaa atcgtcgaag tggtggtcgt cgcgtcgggg
    15001 tgcaccgatc gcaccgctga cattgtgcgc gaacgggccg cgcgcccggg ggttccggtg
    15061 cggctcatcg aagaaccaga gcgacggggg aaagtggcgg cgatcaacgc ctatctgcgg
    15121 gtgttaaatc cgcatgtcga cgccatttgc gtctgtagcg cggatctgtt ggtttcgcgc
    15181 gaagtcgtcg agcgcctggt tcagtgtttc ctgataaacc ccgaagtcgg catgtgcggc
    15241 gggcgaccgg tgccgaccaa ccggattgat tcgtttaccg gcgcagcgac gcgctttttg
    15301 tggcagatgc atcaccgcgt ggcgctcgaa gcccccaagc ttggcgagct ggtgatgatt
    15361 cgcgccggga ttgtgcgcaa cctgccgccc gagagtgcag tcgacgaagc gagcatcgaa
    15421 cagctcgtct gcaacgccgg atatcgtctg gcctatgtcc ccgaagcggt cgtgcacaac
    15481 cacggtcccg aaacggtgcg tgacttcatt cgtcaacggc ggcgaattgc cgcgggccat
    15541 ctgtggctgc gttcggtgtc gggctattcc gtgtcgacga tgaatgtctg gcgcattgcg
    15601 cggctggcgc tggcggaaat tgatgtctca catccgcggg aagcgctgta tcggcttggg
    15661 accattggcg tcgaagcggt gtcgcgcgtg ctcggctttg tcgactttca caccaacagc
    15721 accaaacacg ctgtgtggaa agtcagtgaa tcgaccaagc gggtgatgaa cgacgaagtg
    15781 cgcggcctgt atttgagcga ttccgtcgat gacgcgccgc tcgccaggcc tgagtcggga
    15841 cgcgccaccg gcacataagc tgcggacacg gctacggcgt gttcgcaagc tgcgcaagct
    15901 ccgtcggaag ctgccaggct ttgatcgccg acgtgagcac ttgtttgggc aactgaacaa
    15961 tcaccggcgt ccgtttcggg tcgagccagc gcagcacgtc gaccaggtct ttttcgaaca
    16021 gcgccgtaca gcctgccgtt ccaccctgcg gcggcggtgc gacgtgcaaa aagatgcagc
    16081 ttccggcgcc tggtcgcgct tttggcaggt tgtatccgag caccaccagg tatttgtagt
    16141 ggtccgtcgg catcttcata tgttctgccg atttccacga cggagctgcc gattccctga
    16201 caatttggtt gtagcttggc gacgccacat cgtcgacgca gcgatcttgc gcggtggcct
    16261 ggtgataagg cagcttcacg ccacctggcg ctgccggggc atatccccac atctcgccaa
    16321 tcggcagaat gcctgccggg gaacgtccgt cgccttctcg cttgaccggg ccttccacgg
    16381 gcggaatggg ggcacccgga tcacttcgcc agccaagccc actgcgccca agccagaccg
    16441 gaagtggcga accgacgcgg actagcgacg acgaggactg gcgccgctcg aaccgataga
    16501 gcgtcccgcg cggggcatcg aagccgtcgc tgagcaccac cagaagctgc tgactttgcg
    16561 ccagtgggtt tccgacggca aggctcggca gacagatacc caaaagcaga aacggcgacc
    16621 gcctaatcac gctgcaacgc tcgcagcgcc gtgcgcacca cggtttcgat ctgggcttcg
    16681 ggctgcgctt tgatcgcttg ctccgtcgcg cgctcggcat ctgtgggacg gtagcccagg
    16741 tttacgagcg cccccatcac gtctgcaaac agcccaacat gaaccgcggc gggatcttgc
    16801 ttggtgcggg gctgcggttt gcttctgccc tcgggccgcg gcactttgtc gcgcagctcg
    16861 agcaccaggc gttccgcggt tttggggcca atgccgggca ctttgcgtag gcgcgctacg
    16921 tcttccgcag cgacggcggc aaacagtccc gcggcgtcca aaccggacaa aagtccaatc
    16981 gccagcttgg gaccgattcc tgataccgat gtgcacagct cgaacgcttt tcgttcgtcg
    17041 cgggtggcaa aaccaaagag ctgcatcgca tcttcgcgga cgtgcagatg ggtgaacagc
    17101 tccactttgc taccgacggt ggggagcgtc gccaaggtac acagcgaaac cgatacatgg
    17161 tagccgacgc cgcccacgtc gagcagcacg ctatcgagcg tcttgtctgc gaggatgcca
    17221 gtaagtcgtg caatcacggg tccgactgtg ccaaatcgag cactcgtcgt caaagaagcg
    17281 ggcgcaaaag gatcggggga atggctacag ccagcgtttc cagccctcgg tggtgagctt
    17341 ccagttgtca aggcgtggct tgaggttgct gtcgcgtccg cccacaaggt agtagcggcc
    17401 atttgcgaac gtcatgctgg cgtcgattcg tgccggtgga ttttggtcgc cgctgaccgt
    17461 cgaccaggat ccgccctgca cgttgaaacg gtacgaatcg gacagaaacg aaccgtcctg
    17521 cgctttgccg ccaaacaccg caatcatgcc cgtctgtggg tcaattgcgg cggcgaacga
    17581 ctggcgatag gaaggcgcgc ccccgtcgtt tgatagcacc atccagttgt tgggggtcgt
    17641 ggtggtgtcg agccgccaga ctttcatcac cgtctgcatc gtgtcttcgc agccgacaag
    17701 atacacatcg gtctgcgtcg aaaacagctt cggcccgcgg atgaccggcg caccgtttgc
    17761 cgccgtggtg gcaaagtttg tcatgctggg ggtcagcatg cgccacaggt cactgcgggg
    17821 attgccaccg accccgtcga tgccgccaaa aaagaaggcc agcggcgttc ctgtcatcga
    17881 cggcaggatg gtcagtccgg ctgctcctcg tggtggcagt gggctggtca ccgtcgtaag
    17941 tggcgtccag ctgcgaacgg ctccgggcat cgtcggtcca aactgccatg catcgggctg
    18001 caccacgccg cccgcaccca gaccttgcac cacgagcacg cggtctgccg tcggatcggc
    18061 gaccatctgc ggctggcgcg ggccagggct catcgcgctg gtcgagcgct gccatgtgtt
    18121 ttgggttagg tcatattccc acgtctcgct gagcggctgg tcgccgacga caactccacc
    18181 gaacattacg atggttgtcg gcgccgagat gggcaccgca gccagaccgg caccgtaacg
    18241 cggctcggga ccgataaaag gcagcttgct cacatcgcgt cctaggctca cgacgacgcg
    18301 cttgtgttca cccgcagcga gtgtttcggt ggtgctgccg cgcaagaccg cgtttgcgcc
    18361 tagctcgcca acgatgtcca cttgtgctgt tccaccgtcg ccgagcgcca gggcaaacgc
    18421 aatgggcagg ctgctcgcgg tgttgctggc cgtggcaaat ccctgctgct ggcttgtgcc
    18481 gccgttcacg ctgtagatgg tgcggatgag gtcgattttg acgtcgccct gcgtctcgac
    18541 ttgcacggtg agacagcttg agctttggcc ttggcagggg tcgggcgggc tgcatgctgg
    18601 gctgccgagc gatagcacgg acaaaagacc acagagcggc atacccggtc ggtacgcgaa
    18661 gtgactcatg gattcaccgg attaaaaact gtgacggtgg ggccgcgggt tccaaaataa
    18721 acaccgaggc caatggcggt tccgagcacc accgcgccgc cagcggcagc gccgatgatg
    18781 atccaagtct ttttcgaaaa cggccaaatc gaagacttgg cgacgaagcc tttgggcggg
    18841 ggttcgcttg ctacttcgac ggggaacgtc tggagctggg cgatttcgac cgctggtttt
    18901 gccggagtca gctgctgctc cagctcggcg acagtctgtt cgacttctgc ccgattgggg
    18961 gcagtcggcg cgtcgtttag atattttttg tagtattcga gcgccttttc cggtctgccg
    19021 gctttgttat gcgattgcgc aatattgaag aggaaaaccg gctggggcag gttcttgtag
    19081 cagagctcaa actgctcgat ggccggatcc cattcgcctt ttttgtaatg ggcagttccc
    19141 tgctcatagg cctttttggc ggcagctttg gcagcggcgg tgggcggctc ggcggcgtgc
    19201 acgacaggcg acgccagccc gacaatcccc cagagaagga gcgagcgagc aataactttt
    19261 tccacacctt atcgtatcac gcattgcgtg cgatctgata ggagaagcgc tgtgcaaacg
    19321 gagcgtggcg gcttgcaggt gggggcggtg acagaagtcg tcctgctttt tgttgtgggt
    19381 cttttgctgc tcgtcgtcat cggggtgctc tggggcggaa tgtcgggtcc gctgctgtct
    19441 tggctggggc tgcgggcgct gtcgactggg cgctgggcgg tgattccgca ggtggtcttg
    19501 cccgagcttt tggggctttg cctgccggca tgtctgtttc tgaagtggcg gcaggggtcg
    19561 cctgcgtgcc aagacgtgcc gctggatggt tccagcctgg ggcggctctt gcagcagctt
    19621 gtggccgggc ttctgctcgg cggatcgctg ttttatttcc ttggcgtgtg gctgctgccg
    19681 cactatgaac gactggtgcc gctgtcaccg tcggaacagc gcgcgctctt gcggatgctg
    19741 gtgcctccac agggcttgcg tccgctgtgg attgatctgc tgacgtttgc gctgtgtccg
    19801 gcgctctgtg aagaactgtt ctttcgtggc gcgatcttgc gtcacttgct ggccgcacga
    19861 gcggacttgc ctgcggaaat ggccgcttgg ggcatgcaaa agcgagcgac cgctgtggtg
    19921 ctaagttcct tgctgtttgg aatcatccac ctgtcgtggg gccggcttgt gccgactgcg
    19981 gtgctggggg cggcgttcgc gctggcggtg ctgcgcagcg gttcgctgtg gaccgtgatc
    20041 gcgatgcatt ttgccaacaa tgcggtggtg gtgatgttgg cgcggcaggg catatcgtcg
    20101 ctgacatcgg tgccgatggc gacaagtggg gcgctgcttg tgccggcctg tttggcgctg
    20161 ggactgggga tttggctttt gaaaccacga tcgacccgca tcgactcgcg ttgcagtgct
    20221 tgacagcaag gggcgctgcc gatttgcttg tggatagtgg ctgacttgtt cctgcgacga
    20281 tcggcgtttt gtgcgcgtgt gctgctgggc tttttttggc tcggcctgcc gctgcgcgtg
    20341 tcgatggccc aaaagccggt cgcaacccag tcgccgcttt gggttctgcc ggtcagttcg
    20401 cccccgacgc tgccggggct tggcaaagag cttcagcggg cgcttttggg cgcgctaaaa
    20461 gaagcgaact tgaacgccca gccgggcaag agcgattccg atggcgggat ttcggcgcgg
    20521 ctcgaagagc tgggcacggg gcgattccgt ctgacgctga gccatcgcag tggcagcagc
    20581 cagtcggttg gcgatctcga acacctggat gacttggtct acgcggtggt ggccgagctt
    20641 cggcccaagc tgcattccga gctcttgccg tccgatccgt cgcagatacc gtctcttggt
    20701 gcaagcaagc tggccgcttt gccaagtccg caggtggtca gcaagcagtc gccgacgatc
    20761 cagaccgcgc agtttgggct gggaagcaaa gagatcaagc tggccacggc gctgcttggc
    20821 tcgtccgcaa aatcgaagcg cgagccgcag cggacggaat cagtggtacc ggcgcagctg
    20881 ccgccaccaa cgccggttgc cgtgtcgaat tcggcgacgg ccccgacgcc acctgtgacc
    20941 agcaacttgc cgccggcttc gacgacgaca aatccaacca cgccggcttc agcgcaaacc
    21001 gacccggaac aaaaaccgcg cacccgacca cgaattgccg tcggtgtgat gggggaacca
    21061 ggatcgttgc cacctgggta ttttgggctt gggctgactg ggcagcaggc gctgcttgct
    21121 tttttgcagc agcggcttca agtgccggcg gtggcgacac gtttgtatag tctggtcggc
    21181 ggttttgaag cgctggaaca gtcgctgcgg gtgagcgcgc ggcacacgct gatggcgcgt
    21241 ctggactcac tgtccgtcac gagcggaatc atgagtgggc gcatccatct ggtgcttttg
    21301 cacgacggca agctgcttta cgatcgcagt gttgcgctgc cgccaactgc cgtgagcgca
    21361 gccgagccac ccgcgaccgc gtttgggcga gctgtaacca gcgccgtcga gtctctctcg
    21421 aacgatcttt tgacccgttt gtagtcaagc gggtgccgtt gtccgccgcg gtgagcaggg
    21481 cttcccccgc gagacaaaca gcgcatctgc gggtccggca attgacgcta agtccgcttc
    21541 tgtcatacga taaccttcgc aaaaaggtgt cctcattatc tgcaaccatg agtgggtcac
    21601 cagcaagacg ctgaggagcg cgcgtggcac taccttcgcc ttttggaaaa tacctgctcc
    21661 tcgagcgaat ctccgtcggc ggcatggccg aggtcttcaa agcgaagacg ttcggcgtcg
    21721 agggcttcga gaagatcatc gccatcaaga aaattctgcc ggcgatggcg gaagatgccg
    21781 acttcatcca gatgttcatt gacgaggcca agatcgctgg ccagctcaat cacgcgaaca
    21841 tctgtcagat atttgaactc ggcaaaatcg acgacagcca cttcatcgcg atggaataca
    21901 tctggggcaa ggatttgctc cagattcaga accgtttccg tcgcctgaag aagcacatgc
    21961 cggtgcagat gagcgcgttt gtgatgggca aagtctgtga aggactggac tatgcccacc
    22021 gcaaaaaaga cgcgacgggc aaaccgctca acatcatcca ccgcgacgtt tcgccgcaga
    22081 acgtcatcac cagctacgac ggtgaagtca aagtcatcga ctttggtatc gccaaagcag
    22141 catcgcgctc gtcgaaaacg caggccggcg tgctaaaggg caagttcggc tacatgagcc
    22201 ccgagcaggt cggaggcaag ccgctcgatc agcgcaccga tatttttgcc gtcggcacga
    22261 ttttgtatga gctgctcacg tcggagcggc tgttcatggg cgaaagtgac tttgcgacgc
    22321 tcgaaaaaat tcgcaatgcc acggttccgc ccccgtcgac gatcaacaag gaaattccgc
    22381 ccgaactgga ccgcatcatt ttgaaagcgc tcgcgcgcga tcccaatgag cgataccagt
    22441 ggggcagcga gatgcacgat gatctcgccg actttgcggc gcagtttgag ccggtctaca
    22501 acgccaaagc gctggcgacg tggatgcgcg aggcgttctc gccggaaatg aagaaagagt
    22561 cggcggtcct cgagcagcag cgcaagatcg gcaaagaggc gatgacccaa ggcggacaag
    22621 ccaagggtgg ggcaccgcct tctgcgcggc ttcgttcgcc gacgctggcc ccgacatctt
    22681 cggctccgcg tgcggccccg acggaatcgc ccgatccgac cagtgcgctg tcggaagcag
    22741 aaatcgagcc gcttgccgaa ggggagggcg gtggcgaagg tggcgacctg ctaaaccagg
    22801 cgacggccat cgtcatgccc gatcagcttc ccaatgctgg cgagggcgat ctaccgaatc
    22861 aatcgacggc gatcttcgac agtgccaagc acggcggcgg cgaagacgaa aacgacatca
    22921 aagcgcagtc gacagcgatt tttgatccca gctcgcacaa aggagcgcag ccggcacagc
    22981 ctgcaccggc gccagcgccg ccccagccgc agatggaccc gtttgcgggc atgaatccgg
    23041 gctatccgca aaacccgagc atggtcggcc ccatgggcgc ttttccgtcg gcatatggct
    23101 cggcggtgat gccggcgatg gcgatgccat ttgcgaccgg atcgatgtcg gcgctgccgg
    23161 cgggcgcgat ggtgatgctg cccaatggga cggtgattcc cgcttccgcg gtgcagcagc
    23221 cagccccgcc accaccgccg gttgcgcctg tgcccgaaca gcgcggggga tcgctcggca
    23281 aagacattct cgtcggtgtg ctggtggcca tcgcggtggt tggcttggtg ctcggtggaa
    23341 agtttgcgct ccagcccggc aaaggcaccg tggtggtgac tgtgttccca ccgcgtccag
    23401 cgacggtcta cattgatggt cgcgaagccg gcaaggtcga gcagggtggg gcgctgctct
    23461 tgcgcgacgt cggaaaaggc cagcgtcagc tcttggtgcg agcggaagaa ggcgaatcgc
    23521 agcaagtcat cgatgtgctg gccggtgaca cggtgcaggt gacggcgcag cttggcgcag
    23581 gcagtgcggc agcgcagacc agcggccaag gcaacggcgt cattcggctg cgggttccac
    23641 ccggcgtgtt catttcggta aacggcgtgc tggtcaaaga aagcgacatc ctgaagggct
    23701 atttggtccc ggcggggatg gtgcaaaacg tatcggtgaa aaaagccggc aagcagccgg
    23761 tgaacttcca agtcacgctc cagtcgggtc aggaatacga ccgcgcgatc gagctacagg
    23821 aaggccgcgg caagctggtg gtaaacacca agccggcggg cgccgaagtc ctggtcaatg
    23881 gtcgatcgta tggaaaatcg ccagtcaccg tcgaggatct cgatccaacc aagaccgcca
    23941 aagtggtgct caaaaagcgc ggacatggat cggtgaccaa gctggtgtcg ttcggcgatg
    24001 cgttcgagca gagcctcgac atcgactttg cgacgggcgg caaggatgac gacaagggct
    24061 cgtcgaagag ttctggttca tcggacagcg cccaggcgag cagtgccacg ccggccaaaa
    24121 ccgaaacccc tgcggcggcc tcttcaagtg gcgacaaaga aggttttttg atcgccaaca
    24181 gccagccata tgcgcgggtg ttcgtcgatg acaaagacac cggcaagacc acgccgatcg
    24241 cgccgcgtga tcgcattccg ctcaaggccg gcaaacacaa ggtcacgttc gtgaccagcg
    24301 gcaagcgcgt aaacttcgac gtcgtgattc gtgccggcga ggaatacaag ctggtccgaa
    24361 acctcaacga agactaaaca cgcaacgccg aaatgccagt ggacgagggc tgctgtggct
    24421 tggcgctgtc agccggcacc ttggccgaac ctagcgcgcg gtcacgagca cgaacacacc
    24481 caaaaacagc agcgttccga tgatcatcgc gacaagttcg atgattccga tgcggagtct
    24541 gtgcgtcgga aactcatcgc gcaaagacag ctcgaaaccg gcgacaagac cgagcggcac
    24601 aagccacgaa aaatcgaacg ccagcgacaa gaacagcaca cacaaaagca ctccgagcag
    24661 cattgcgagc agcgtctttg cgatgtgctg aatttcctcg agccgcgcag gctcggtcgc
    24721 caagagttct tccgacggaa actgaatgac ctggatgagc agcttggccg acgcataaat
    24781 gcccgttaga tacagcgctc cgccgatgat ccccgtttcg accagcgcct gcaaaaagct
    24841 gttgtgggca ttcaaaaagc tgatccgttc gtagttcgca aaaccgacgc cgaacagcgg
    24901 atagtcgagg aaagcacgga agccatttag gtacgtcatg atccgcgtca ccgtcgaata
    24961 agcggcttcg gcttcatttc gcgtcgaaat cagcagcagc ggcgacgaca gcacggctgc
    25021 gaggacggct cccgatatgc cgacgcgaaa atagaaaaaa cacagcagca ctgcgcccat
    25081 cgctacctgc gccgctcgcg acgcggccaa aatcaccgcc aggcccagca ccggcaagcc
    25141 gataagccac aaccagcgtc tcggcgacgg gtcgggccag gttgccagtc ccaccagcag
    25201 cgccgctgcc atcaccagtg ccagcgccag cgcattcgga tcgagcaggt tgccggtgta
    25261 gtggatgcgg tccaccaccg tcgccagtcc cagcggtccg gtccgttcgc acgcccagcc
    25321 gaagtcgcgc aggtgctgct cgtgctgggg cactgtgtag cactcggcgg tcgactgaca
    25381 ggggcgccgg tcgctcgtct gttcatagtt tagctgcgac gcaaagcgga aatagtggca
    25441 ctgcttgggc ccaaatcgct gcggaatcga gacgatcgcg gcaaacagca tcgcaagcac
    25501 gaacgtcgcg tagatgactc gctggcgccg tggattggtc gtcagcacgc caagcagcgt
    25561 cgaaaagacc aagtctttgg catagtcgcc gatcagaaag ggcaaaagtt cgtcgacccc
    25621 atccgatacg agccgcgaaa tcagcgccca taaaa
//
LOCUS       DAORGN010000052        25391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_758_length_25391_cov_6.255328, whole genome shotgun sequence.
ACCESSION   DAORGN010000052 DAORGN010000000
VERSION     DAORGN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 25391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25391
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_25391_cov_6.255328"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKL17_08030"
     CDS             <1..256
                     /locus_tag="PKL17_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44715.1"
                     /translation="RLAEGESLLVVLTGSLRGARARTVDVQFAAAPTPPIDKMPDTDP
                     PMASGCAVSGRSSSWSPGPIGLLLCALAFVRRRFLPWARR"
     gene            320..1702
                     /locus_tag="PKL17_08035"
     CDS             320..1702
                     /locus_tag="PKL17_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNK44716.1"
                     /translation="MGIIVQKFGGTSVGTAERLRNVADIIEKTYRTKPVIAVVSAMSG
                     TTKAEGTTSLLLAASAAAQTTGSPFRHELSLIEAVHVRAVEQAITGESLRAEVLHFVR
                     DEIEKLQRFLDAIRVIRELSPRSQDVVMGLGERLSARVLAAVLLDRGLPSRFVDLAQV
                     VPENEKEVDPAFFRRLQTRIGELCRPQGRELPVVTGFFGMVPGGLLGAVGRGYTDFTA
                     AMIAAGLGQSLADEMQVWKEVDGIFTADPRKVPTARVLPRISAAEAAELTYFGSEVLH
                     PFTMERVVSAQVPIRIKNTFKPDAPGTVICSHEEARPGCVTAVTVKRDICVVTITSNR
                     MYNAYGFLARVFGVLDRHGVVVDLVSTSEVSISCTVDKLADAEKARADLEQLGQVFVS
                     PRRAILAIVGESMKYTTGTAGKLFSTLGDSGISVEMISQGASEINISCVVKEEDAARG
                     LCAIHAAFFG"
     gene            1785..2138
                     /locus_tag="PKL17_08040"
     CDS             1785..2138
                     /locus_tag="PKL17_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44717.1"
                     /translation="MKKLLAFLVVVALVGGAGFFGYREMRPERRACRKLASLCERVDK
                     STTAEENSKSCESTFAELRDTSGPDSVDKPAQCILDSKTCPEATGCFVGGVGGGLIRG
                     LGSEFLKGLGKSLGD"
     gene            2156..2329
                     /locus_tag="PKL17_08045"
     CDS             2156..2329
                     /locus_tag="PKL17_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44718.1"
                     /translation="MVRSIRLSSSSQNAPRAFGNAVADSGFLFPTTSRAITTQNPVLL
                     ACMNGMWNVLPPD"
     gene            2446..3714
                     /locus_tag="PKL17_08050"
     CDS             2446..3714
                     /locus_tag="PKL17_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK44719.1"
                     /translation="MAALQTTANGQTEGTLLCLAALFERLAHFVVISLLALHLNERRG
                     YSEPDALSVCGLYGAAVYVSAILGGWVSDRSGNPRLATQIGAFLLCGGYVILASPTLP
                     IVVPLALLTCGHALYKPSLAVLFGRLRGQSAEARFRLLYLTVNVGATLAPFAAQLALS
                     RGGAMAPFVLANLLSVVTLATVLILSKMPDSVSEQTVSRESAQGHDKKTPNIGTVLIL
                     CLLSLAFFSALAQTEGALIFWARDRTDRSLFGYEIPTLWLTCLPALCLLLIAAPIERL
                     WLLLTKWGRPLSAQNKLRLGLLVTAVAFAMFALTARAAETSGKLAMRNMVFLNLLLAV
                     GELCVAPVAMAIVHSISPGKRQGLGMGLWFLVVALANWLAGQLGRLSTDSRSAHPYAA
                     WALLALGLFVAWLVSVLFWTKLDDKPDSVE"
     gene            4032..5225
                     /locus_tag="PKL17_08055"
     CDS             4032..5225
                     /locus_tag="PKL17_08055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK44720.1"
                     /translation="MDRKRIAIVGTNFAGYTAAIELKELVGDNHDIVVIANTHKFLFF
                     PSLIWYPFGLREEKDITFDVRPIYQSHRIGFIEAEVTGFDPEQNRVLLKDQEPVTYDY
                     LIIGTGPKVDYDYIPGLREHSQSIVGLAPAHRTREAWNKFLSDPGPVVIASAQGAACF
                     GAAYEFLFNVRYQLSKHHLQDKAPLSYVTAEPFAAHFGIGGFGNAQKMCEWMFSHYHI
                     SPHYNAVIQEVRADGVVLADGTFFPSKFTMVMPRFVGIDAVRNTKGLANANGFLEVDD
                     SYQHPTYRNIYAAGVAVAVKPPSETPVPCGVPKTGYPTEQMAKTAVKNIIADIKGLPK
                     QHAKFDDMAAYCIMDTGNMGMVIVGDHMLSPREHEFIIPGPEAHWAKVAFEKYFLYSR
                     RHAHV"
     gene            complement(5270..7729)
                     /locus_tag="PKL17_08060"
     CDS             complement(5270..7729)
                     /locus_tag="PKL17_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK44721.1"
                     /translation="MMSPPSIPSHARVVIIGGGIIGTSVAYHLAHMGVKDVVLLERDR
                     LTSGTTWHAAGLIVTFGSTSETSTELRKYSRDLYTRLEEETGLSTGFRPVGFIELAAD
                     ADRLEEYRRVAAFNRHCGVDVHELSGREVQALFPLARVDDILAGFYVKEDGRGNPVDI
                     TMALAKGARQQGAKLLEGVPAIGITHKRGVVTGVRTPYGDIQAEYVVNCAGMWARQLG
                     DEAGVNIPLQAAEHYYLITEKIPQISPDWPVIEDPGSYGYYREEGGGLLVGLFEPVCA
                     PWKTEGIPSDFSFGEISPDWDRMGPYLQKAMERVPISLETGVKKFFCGPESFTADLSP
                     VIGEAPELKNYFVAAGLNSIGIITGGGMGRLIAHWILHGRADVDITGMNIDRFHRYQS
                     NPEYRRVRTVESLGMVYQCHYPTRSMMTARNCKRSAFHDRLVAQGAYCKDVSGWEGPD
                     WYAPPGVEPRVEKLSWGRQNWFPYWEAEHRATREGVILMDMSFMAKFLVQGHDAGRLL
                     NYLSANQVDGPRGVITYTQWLNEAGKLEADLTVTKLDDERFWVVASDTAHRHAETWMR
                     RHFGSHHAFLTDVTSGYAQLNVQGPLSRTLLQRVTTVDLSNDAFPFRTARDIDIGFAR
                     ALCIRITYLGELGYELYIPTEQALHVYDRLMEAGRDLGVRPAGLKALASLRMEKGYRD
                     YGHDIDNTDSVLEAGLGFAVDLKKPEGFLGKAAVLAKKAEGPLKKRLLQVQVLDPDPL
                     LFHAEVVYRNQRALGYIRAASYGHTLGGAVGLAMIEADCAIDQDFVDKGQWEVDIAGK
                     KYPAVASIRPLYDPDMKRIKC"
     gene            complement(7749..8417)
                     /locus_tag="PKL17_08065"
     CDS             complement(7749..8417)
                     /locus_tag="PKL17_08065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44722.1"
                     /translation="MLNWAARYYPIVRALRRVVGNTDSILEVGSGAFGLARYLPHNIV
                     GCDVTFAQKPLANLLAVRASALHLPFSDESFAAVVSSDVLEHVPPDLRPQVISETLRV
                     ARKVAIFGFPCGPLAQKLDEDFLTFHREHNIDPPEWLTEHMQFPFPQTSLFAQLEPAW
                     RVRFFGNESLRFHALINHAERRGPWNRLFQTGLSLVPTLCERALQLFDRPPFYRQICI
                     IERA"
     gene            8522..9883
                     /locus_tag="PKL17_08070"
     CDS             8522..9883
                     /locus_tag="PKL17_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNK44723.1"
                     /translation="MKLRATLRGKTYQFRDLRDVMAKANEEKSGDRLAGVAAADTLER
                     IAAKLVLSEIPLSAFVEHPAIPYEDDAVTRLILDELNQPVYQSVKNWTVSQLREHVLR
                     TDVTGPDLLRLGRGLSSEMIAACAKLMSNLDLVCAARKIRVVVHANNTLGQEGRLGVR
                     LQPNHPNDSIDGILASLRDGLSFGCGDAVVGINPVTDNWEITRKILDATHDFLEEWQV
                     PTQNCCLSHVTTQMKALQKGAKLGLMFQSLCGTESGLRAFGTDIGMLREAWDMTRKLG
                     TATGPNVMYFETGQGSALSANAHHGVDQLTCEARNYGIARYYQPFLVNTVVGFIGPEY
                     LFDARQITRAGLEDHFMGKFHGLPMGCDACYTNHADVDHNDLENLEILLANAGCNFFM
                     GLPMGDDIMLNYQSTSFHDDATMREVLGLRPAPEFEAWMERVGLWENGRLSARAGDAT
                     LLT"
     gene            9885..10358
                     /gene="ybaK"
                     /locus_tag="PKL17_08075"
     CDS             9885..10358
                     /gene="ybaK"
                     /locus_tag="PKL17_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HNK44724.1"
                     /translation="MNDIPVTTAVRFLRAHKIDFKPHLYDWEEHGGTAHSAKSLGVDE
                     HQVIKTLVFEGDGKKPLIVLMHGDREVSTKNLARFLGCKTVQPCTPQNVTRYTGYQVG
                     GCSPFGTRQSLLVYVEQSILTLPRMFINGGKRGFLVELFPTALSHLSPTQVTVGI"
     gene            10482..11003
                     /locus_tag="PKL17_08080"
     CDS             10482..11003
                     /locus_tag="PKL17_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNK44725.1"
                     /translation="MRTAKELLSNYAEYHRDRRNIATHFVGIPMIVFSVAVLLSRLHI
                     PLGPQWLSAATVTWVLTTVFYLSRHLVLGGATSLAYGLAIWFAQGYANGPFSTWISVG
                     VGCFVVGWIIQFIGHYYEGRKPAFVDDLVGFLIGPMFVVAETLFAMGVCLELHEHIVA
                     TAGPTRIRDMSRA"
     gene            11007..12131
                     /locus_tag="PKL17_08085"
     CDS             11007..12131
                     /locus_tag="PKL17_08085"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNK44726.1"
                     /translation="MSGHSSPEPKVSPVELFFDLVFVYAITQVTTLLLSDLSPLGFVR
                     SGLALFMIWWAWSLYTWMANAIGPSGRATRAGILVATVPTFMMAFTLPGLFRDTALWF
                     GGSYFVVRELGLLLYWIFLSHDREHRSGLRLFVPYASLAPMLVLAGGFVSAETRTLLF
                     AAAMGIDVLGALSSGRGLFRISAAHFAERYGLIVIIAMGESVIAIGIGAAQAHCDAWL
                     LGGITVMLLGVIALFWSYFDWVSEAAEHRLVHAKEADRGRLARDMYTFFHFPIVAGTI
                     LYAVAAKKILAHAHDPLSLSGRFCLSAGIGLFLLGFVLGSRRVGGLVMYERLLAISLI
                     AALSAVPLAIPGLGLAALVLFVLACALALENRRRKRHPAH"
     gene            12234..13133
                     /locus_tag="PKL17_08090"
     CDS             12234..13133
                     /locus_tag="PKL17_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44727.1"
                     /translation="MNARKHLSRFLALSVMSLLMLISASAQATNYTLWIHGRTASTTK
                     PGNYADFSYWGPATVSAGVNKKAVNWDGVSYISSQNYRVRDALDCYCTGTNWCYIAVH
                     SAGDYQIGYALSMYGGSARYKKNATPSSTGTCGNTDGTTQTGWNIKWVRVASGASGGS
                     ELADAGSWASSEQLTQDLVTTTARAKYNHNTTYGVTFYNYAGASGTLYSAILPGQDDE
                     AVAYHTTGGVAGTAGASFGNPSDWFSNDLTLGTAACEGGAAKWSYHSVSYRDDAEALN
                     HYASGNWAGIIGPVRTDVAKYAL"
     gene            13151..14341
                     /locus_tag="PKL17_08095"
     CDS             13151..14341
                     /locus_tag="PKL17_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44728.1"
                     /translation="MALGRNKTLGLGAVFAVIVGAAYFLSRPEPSLPLPEGSPASPSA
                     PPVFAGRLQAPSGTLSGVAKPQPESTADDWPQRLREAQERLGNYKRFARYPPGSRPAR
                     EHPDQMTPFLPVVRQSPLILHGEANEDVRIQLGQDRRELVADEAAKLWVRCEDSQGKV
                     LPCKVAQATAMPAPPSDSKLPPVSLSFSDDGQNGDEHAQDGTLTTSFQPSLLGFRSFH
                     GTMRVQVQLALGKEQGSALFDLLYTPTAPARFTGTVKESRADGSLQFVLGLSVQKAGR
                     YFVVGRVDDQKEKQIGYLQWEGELAPGNQTVSLTVFGKLIYDERPEFPLRLRDVEGFL
                     FLEDSAPDRMHMPRLMGVVHKSQVYSVADFADTEWDGEMKRRYLDEYSKDVERAEKRD
                     PNLP"
     gene            14402..14872
                     /locus_tag="PKL17_08100"
     CDS             14402..14872
                     /locus_tag="PKL17_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44729.1"
                     /translation="MAASSDQDPAVEITFGRHRGVFDGERAVITVHGVVTESDAAQMM
                     DFILRWDARAGNLGMVLRAPESFSVTPEARKYFVRSSRVGLPAIPMAVMGTSAIVRGI
                     LTLLINAMRITLQRDVPVRFCSTDEEALSWLSDQMKLRAVQLANMKVIAATPKS"
     gene            complement(14924..15379)
                     /locus_tag="PKL17_08105"
     CDS             complement(14924..15379)
                     /locus_tag="PKL17_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44730.1"
                     /translation="MEQQRPLPLIPPEIGSMRLRLHDDILHVHSDGHLDLAKSKQITE
                     VYQSVLDHFGYLLLRLDLTHSTGIDLDARRHAVEWGKGCIDVHATAATGAPLAIRMFV
                     GLMFRATQILAGNSTSAMHFASGEDDAVKWLSLQRPRLITASVKRRPSR"
     gene            complement(15442..16653)
                     /locus_tag="PKL17_08110"
     CDS             complement(15442..16653)
                     /locus_tag="PKL17_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNK44731.1"
                     /translation="MRAGSLNIWGPYQDAPNKFRLKISENGEVRSICFRSYDEAELAK
                     ARLRDDIAAQHVYTVGEAIRQFTENLQETRGIKPESVLWTAAQLEWLPSETPLASVNK
                     ERAEKLYRDLVTAPNPRTGKPLSTATHHGRLSLARRLYEFAIKNEMCRHNPFASVTPV
                     GRKRVGKPQLRIDEARRFEAKALELAQAGDVAALGALLMLHLGLRQGEVAARVARDVD
                     DEGRVLWVPSGKTKNARRRLKVPDVLRPLLLKLIETTPPGALLFYKGDKVPPTNYFWS
                     QVRRLCQLAGVPAVCPHSLRGLHATLALEGGATADAVARALGHGSFAMTARHYASESS
                     VDNAKASKVSSLLGSTPSSHLSTADTAKQATPHPNPQPASDDSDQLIGVLRSLPADKL
                     AAILSRLAPTG"
     gene            complement(16813..17187)
                     /gene="folB"
                     /locus_tag="PKL17_08115"
     CDS             complement(16813..17187)
                     /gene="folB"
                     /locus_tag="PKL17_08115"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNK44732.1"
                     /translation="MSDRICIEGLTAFGLVGVYPNEKDTPQRLLVNLCVETDTRVAAH
                     SEQLEDTLDYDRLAECVRQVIRERHHNLIETVAERIALRILLAHRGRIEALTVRVEKP
                     GAVPDAQTVSVEIRRTMADMNP"
     gene            17289..18266
                     /locus_tag="PKL17_08120"
     CDS             17289..18266
                     /locus_tag="PKL17_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HNK44733.1"
                     /translation="MSTLAITLSDILAAQARILADVHHTPILRCQTLSSLCGCTLGLK
                     AEHLQRSGSFKLRGALNRLRQLSLAEKQRGVVAFSSGNHAQGVALAAQICDLPAVIVM
                     PSDAPKVKLDATRGYGAEVVLYDRKTEDREAIARKLCTERGAILVPPFDDPHIMAGQG
                     TVGLEILSDVPDVDTLLLPIGGGGLISGVAMAVKSLRPSVRVIGVEPETADDALRSRQ
                     AGQVVRISQPQTIADGVATQAVGQLTFPVMQALVDDIVTVSEAQIAAALGLVLTRAKQ
                     VVEPTAALGVAALLSGRCRLPGQNVVALLCGGNLDLSSLPRIQELAGIR"
     gene            18302..20182
                     /locus_tag="PKL17_08125"
     CDS             18302..20182
                     /locus_tag="PKL17_08125"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK44734.1"
                     /translation="MLEALGPGPLSPVTARFRALVSALLSPALPVRLDSQNSTEVQLR
                     IERPGKPPEFVVIVDAVKEDERSLQLRLARLTSESVNDQHVVVLSNQGWLPDVLTKAA
                     ATYGSRLKLYQVDSEGKCLLGSPGGALAAALKAQKELPPSQETEEEFAKRISEAESEK
                     QRQIDRFEERLTAQSPVATFVLAGLLALLYGLFRLWTPADGALWTQLQLGICVPRFVS
                     DGELWRLVTGGLLHDQLLVVGLNIAVLLSLGYQTERLIGTGRFLVLVVASLLAAALFA
                     VIPTPQLLIPRKYGFSPALFGIIGGLSGLSILPRSLPPETVARVRKVALAGVIPGAIL
                     AVLSGERGTLLAGFAVGLILVGSGLLPSLRLSDPDPPSGVTRLAQQAMVGLSALLLVG
                     SLAVAFVVGKPWSQEQSARERFSRTMLAQALGNGKSGPAGQSGSATATGGATPEADMT
                     PQLQSLPGVGIGLVLPKLLGDPQLQGEGAQQTIRFGDLTGKLQQLDVAVRRHEAPFKK
                     RAQVLVAFDNAVALVKNDKQREVKAGRLTLVGEPRSFEVGDLPALEMTFNGVEGQKAR
                     TAVFVNKQALIVLSYLYSDLLPPNLQLDLGKVLSSVKDEFADSPVEQAKGKKKKKAKK
                     RR"
     gene            20243..21055
                     /locus_tag="PKL17_08130"
     CDS             20243..21055
                     /locus_tag="PKL17_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK44735.1"
                     /translation="MELSSSSHRFAVYPSLAGKVALVTGASSGIGAACAEALAQNGCR
                     LIVLARREERLRSLADRLTSLGAPDVWPVVCDVADRPQVMAQLAALPSRLAAVDIVIH
                     AAGLGRGRDKLHEGNPAEWDEVLDCNLKGVLNVTHAVLPQMVARDAGHIVHIGSVAGS
                     QPYQGGAVYCASKAAIRMLNQCLKHDLLGTRIRVSSVDPGMVETEFSIVRFRGDIDRA
                     RAVYEGMTPMTAADVAESVLFCLSRPAHVNISEVMMLATDQAAATTVHRRPR"
     gene            21296..21943
                     /locus_tag="PKL17_08135"
     CDS             21296..21943
                     /locus_tag="PKL17_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44736.1"
                     /translation="MATQIIDVDSEDKFPRIHLIDHTGPGGYGGFIVLTGSDITNFKG
                     YLKKYSGHRLVSKYAGIHIYWGTYTRAKIGKDEIYDSMNLLHQGWGVKTISAVSTIQS
                     FAQATTVTQFAQKGAMKVIPYLIPSGDLPSPRDLLAEKNRPYKRGDTKKHIFSDFMSL
                     LGRAGFASRYFLSISYRDGSSGHAGGLRGQEKIAMLGYVDANTSINFEQIAQLYF"
     gene            complement(21966..24377)
                     /locus_tag="PKL17_08140"
     CDS             complement(21966..24377)
                     /locus_tag="PKL17_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44737.1"
                     /translation="MNLLGGDWNEWKLTLLAPRSLSGRIVALLIVVVVLGLAVRALRG
                     EQPRRRLLLLSLRTVGLLATLLLFFQPTIRLRNVSLQPNHIAVLVDSSESMRLAEEGG
                     GETRAARAANHLRSQLGALGQWQKDHKVEFYSFGERLQSATWDSVTHPDPANLRADAT
                     RIREALSAVRQRYEGRDLAGVVMFSDGLDNGRLSSGLSFPNPEQLSFDADTQEFLRAL
                     DVPVHTVAVGKPGLRDIALTRVLADQFAFARTAVEVEARLQVFGAKAAGFAGRHLPVT
                     LRRDGVPVLTVDIEVQPEQRDYRVVFPFTPDRVGKFLYDISVPVQPGEAISENNRRAF
                     VLKVVREKIRVVHVSGRPSWDARFLRAALKSDPNIDLVAFFILRSPSDIEFAPSEETS
                     LIPFPCQELFQDQLRSFDLLILQNFNYSAYCSQFLQPAIVPFVEGGGAVAMLGGDLSF
                     SSGGYYGSEVSQILPVVLPPDELSGAPSSRLIDADPFVPKLTPAGRNHVVTALHLDVK
                     ENAQRWEQLPPLDGLNLVQKARQQATVLLSHPRLKDQDGNPMPVLSVAELGKGRTLAL
                     QADSTWKWAFAPPQTGKAAAKAEGDTRSTTYQRFFDQAIRWLIRDPSLRLLRVEVAET
                     ELRRGQPVRVEVRALGPDYQPVGKVEVSLSLQRIAGTESGATSEPLAESVMSKTLHTD
                     EEGVGSIEWPQLPEGGYRVIARAVIAGRPTDDSDVFLVRGIGRELEAPEADDRLLKLV
                     SETTGGDHKKPGDSLMGLSFHPPHIEHVRDHRDIPLWDNPLVLAIAVTCFGLNWTLRR
                     RWGYA"
     gene            complement(24374..25189)
                     /locus_tag="PKL17_08145"
     CDS             complement(24374..25189)
                     /locus_tag="PKL17_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNK44738.1"
                     /translation="MRLRSQLGGLFLLLLACFVAGPRLALGFGGEDKLELGQIQLTGL
                     PTAQAIPRPEALSRLLWELEKRTSVEVNSDSQIVHLGDVASLHRHPLLYLAGDRAFPM
                     PSDDELANLRRHLQLGGMLLIDSAEGRAGGAFDQSVRALLTKLFPQTPLGRLPDDHVL
                     FRSFYLLRVPIGRIIALPYVEAVQLRGRAAVVYSQNDLGGAWARDRYGQWLHDVVPGG
                     EVQREQALRLGINLVLYSLCLDYKADQVHVPFIMRRRAWQPQLPSITPAPVTP"
BASE COUNT         4966 a         7262 c         7850 g         5313 t
ORIGIN      
        1 gcgcttggcg gaaggggaat cgctgctcgt cgtgctgacc gggtcgctgc gcggggcgag
       61 ggcgcgcacg gtcgatgtgc agtttgccgc agcaccgacg ccacccatcg acaaaatgcc
      121 tgacacggac ccgcccatgg cgagcggctg tgcggtgtcg ggacgttctt cctcttggtc
      181 gcccggaccc atcggcctgc tgctgtgtgc gcttgctttc gtccgtcgtc gcttccttcc
      241 gtgggcgcgg cgctgacggc gaaattttgg tgcgctctag acttgaccgt gcgcgcccat
      301 ggcctcatga ctttttgcca tgggcatcat cgtgcaaaaa ttcggcggca ccagcgtggg
      361 cactgccgag cggctccgca acgtcgctga catcatcgaa aaaacctatc gcacaaagcc
      421 ggtgattgcc gtcgtgtcgg cgatgagcgg gacgaccaaa gccgaaggaa cgaccagcct
      481 gcttctggcg gcttcggcgg cggcacagac gacgggatcg ccgtttcgcc acgagctgtc
      541 gctgattgaa gcggtccatg tgcgggccgt cgagcaagcg ataaccggtg agtcactgcg
      601 ggctgaagtg ctgcactttg tccgcgacga aatcgaaaag ctccagcgct ttctcgatgc
      661 gatccgggtg attcgtgagc tgtcgccgcg gtcgcaagac gtggtgatgg gtctgggcga
      721 acggctgagc gcgcgggtgc tggcggcggt gctcttggat cgcggtctgc cgagtcgctt
      781 tgtcgatttg gcgcaggtgg ttcccgaaaa cgaaaaagaa gtcgatccag cgtttttccg
      841 acggctgcaa acccgaatcg gcgagctgtg tcgtccgcaa ggccgcgagc tgccggtggt
      901 gaccggcttt tttggcatgg tgccgggcgg actgctcggc gcggtgggcc gtggatacac
      961 cgactttacc gccgcgatga ttgctgccgg attgggtcag tcgctcgccg acgaaatgca
     1021 ggtgtggaaa gaagtcgatg gaatcttcac cgccgatccg cgcaaagtgc cgaccgcccg
     1081 cgtgctgccg cgcatctctg ctgccgaggc tgccgagctg acctatttcg gttcggaagt
     1141 gctgcatccg ttcacgatgg agcgcgtcgt gtcggcacag gtgccgatcc gaatcaaaaa
     1201 caccttcaaa cccgatgcgc cggggacggt gatttgcagt catgaagaag cgcgtccggg
     1261 ctgcgtcacc gccgtgacgg tcaagcgcga catctgcgtc gtcaccatca cgtcgaatcg
     1321 gatgtataac gcctacggtt ttttggcgcg ggtgttcggg gtgctggacc ggcacggcgt
     1381 cgtcgtcgat ttggtctcga cgagcgaagt cagcatttct tgtaccgtcg acaagctcgc
     1441 tgacgcggaa aaagcccgcg cggatctgga acagctcggg caggtgtttg tgtcgccgcg
     1501 tcgcgccatc ttggccatcg tgggtgagag catgaagtac accaccggga ccgccggcaa
     1561 gctgttttca acgctcggag acagcggcat cagcgtcgag atgatttcgc aaggggcatc
     1621 ggagatcaac atctcgtgcg tggtgaaaga agaagacgcg gcgcgcggtc tgtgtgccat
     1681 tcacgccgcg ttttttggct aaacatccgt cgctgttgct tggcgacgcg gcttgtccta
     1741 cccttgcgat gggtttttgg tttagccatc acggaggctc gctcatgaaa aagcttcttg
     1801 cgttcctggt cgtggtcgcg ctggtcggcg gcgctggttt tttcggttat cgggaaatgc
     1861 ggcccgagcg gcgagcatgc cgaaagcttg cttcgctgtg tgagcgggtc gacaaaagca
     1921 ccaccgcgga agaaaatagc aagagctgcg aatcgacgtt cgccgagctg cgcgacacat
     1981 cgggccccga tagcgtcgac aagcccgcgc agtgcatcct cgatagcaag acctgccccg
     2041 aagccactgg ctgttttgtc ggtggcgttg gcggtggtct gatccgcggc ctcggcagtg
     2101 aattcctaaa gggactcggc aagtcgctcg gcgactaaca ccgtttgctc atgtgatggt
     2161 gcgctcgatt cgcttgtcgt catcgtcgca gaatgcaccg cgcgcgtttg ggaatgcggt
     2221 cgccgattcc ggattcctat tcccgaccac atcacgagcg atcaccacgc agaatccggt
     2281 tcttttggca tgcatgaacg gcatgtggaa cgtactgcct ccagactagc tcgctctgtc
     2341 gacgacaaat agcttggtgc atgcggcgga tggctgggaa aactccaaat tctctggctg
     2401 cttgagctat tttgtgagat cgcgcaccat cgaaaacagg ggctgatggc ggcgttacag
     2461 accaccgcga acgggcagac ggaaggaaca cttctgtgtt tggctgcgct gtttgaacgg
     2521 ctggcgcatt ttgtcgtgat cagcctgctt gcgctgcacc taaatgaacg gcgcggctat
     2581 tcggaacccg atgcgctgtc tgtgtgtggt ttgtatggcg cggcggtgta tgtgagcgcg
     2641 attttgggtg gctgggtttc cgatcgctct gggaatcctc gcctggccac gcagatcggc
     2701 gcgttccttt tgtgtggtgg gtatgtgatt ctggcttcac cgacgctgcc cattgtggtt
     2761 ccgctggcgc tgctcacatg cggacacgcg ctttacaaac cgagtctggc ggtactgttt
     2821 ggtaggctgc gcggacagtc ggcggaagcg cgattccggc tcctgtattt gaccgtgaat
     2881 gtgggggcta cgctggctcc ttttgctgcg cagcttgcgc tttctcgcgg tggcgcgatg
     2941 gctccgtttg tattggcgaa cttgctgtct gtggtcacgc tggcgacggt ccttatcctg
     3001 tccaagatgc ctgactctgt ttcggaacag acagtgtcca gggaatccgc acaaggacac
     3061 gacaaaaaaa cgccgaacat aggaacggtg ctgattctct gtctgctatc gctggcgttc
     3121 ttttctgcgc tggcacagac cgaaggggcg ctgatttttt gggcgcgtga cagaacggat
     3181 cggagtctgt ttgggtatga aatcccaacg ctgtggctga cttgtctgcc ggctctgtgt
     3241 ctgctgctga ttgcggcgcc tatcgaacgg ctgtggcttt tgctgacaaa gtggggtcgt
     3301 ccactgtcgg cgcagaacaa gctgcgactt gggcttttgg tcactgcggt ggcgtttgcg
     3361 atgtttgcac tgactgcgcg cgctgccgaa acaagcggca agctggcgat gcggaacatg
     3421 gttttcctga acttgctgct cgcggtggga gagctgtgtg tggccccggt ggcgatggcg
     3481 attgttcatt cgatctcacc gggaaaaaga caaggtctgg gaatgggtct gtggttcctg
     3541 gtcgttgccc tcgcaaactg gctggccgga cagcttggcc ggctgtcgac agattcgcgc
     3601 agcgcccatc cctatgcggc gtgggcgttg cttgcactgg gactgtttgt ggcgtggctg
     3661 gtaagtgttc tgttttggac aaagctcgac gacaagcctg attctgtgga ataagcggaa
     3721 gctggcacgt agcttgcaaa acatccatgc aggagcgtcc catggatgtc acgaacctga
     3781 ccctgcttgc tggcgctgtg atctggatcg tccttcagtt ttggctgctg ccccgtctgg
     3841 gagtccctac ttgagcggtt cctagcgcgc gccggtcggc gtgcagtcgc caggcagagt
     3901 ccgatccctc tgtgaaacag aatgaaacag agtccggaca gaaacagtaa catttcgtcg
     3961 acaaattgtt tcacgaaatg aaacagagtg tgaaacacaa gactctgttt cggtggaaag
     4021 ggaaagaaaa catggaccgc aaacggattg caattgtcgg tacaaacttt gccggctaca
     4081 ccgctgcgat tgagctcaag gaacttgtgg gggacaacca tgacatcgtg gtgatcgcaa
     4141 acacgcacaa gttcctgttt ttcccgtcgc tgatttggta tccgttcgga ctgcgcgaag
     4201 aaaaagacat cacgttcgat gtccgtccga tctaccaaag tcatcgcatc ggcttcatcg
     4261 aagccgaagt gacaggcttt gatccggaac aaaatcgtgt gctgctaaaa gaccaagagc
     4321 cggtgacgta tgactacctg atcatcggca ccgggcccaa agtcgactac gactacattc
     4381 cgggtctgcg cgagcactcc caatcgattg tgggactcgc ccccgcgcac cgtacccgag
     4441 aggcgtggaa caagtttctg tccgatcccg gtccggtggt catcgcgtcg gcgcagggtg
     4501 cagcctgttt tggtgcggcg tatgagttcc tgttcaacgt ccgctatcag ctttcgaagc
     4561 atcacctgca agacaaagca ccgctttcct atgtcacggc ggaaccgttc gcggcccact
     4621 tcggaatcgg tggctttggc aacgcgcaaa agatgtgcga gtggatgttt tcgcactacc
     4681 acataagtcc gcactataac gcggtgattc aggaagtcag agcagacggg gttgtgcttg
     4741 ccgacgggac attcttccct tccaagttca cgatggtgat gccgcgcttt gtgggaatcg
     4801 atgcggtgcg caataccaaa gggctggcga acgcaaacgg atttttggaa gtcgacgatt
     4861 cctatcagca cccgacgtat cgcaacatct atgcggcggg tgtggcggtc gctgtgaaac
     4921 cgccttccga aacgccggtt ccgtgcggcg ttcccaagac cggctatccg acggaacaga
     4981 tggcgaagac cgcagtcaaa aacatcatcg cggacatcaa ggggcttccc aaacagcacg
     5041 cgaagtttga tgacatggcg gcgtactgca tcatggacac gggcaacatg ggcatggtga
     5101 ttgtgggtga tcacatgctg agcccgcggg aacatgaatt tatcattccc ggtccggaag
     5161 cccactgggc caaagtcgcg ttcgaaaaat acttcctgta ttcccgtcgc cacgcccacg
     5221 tctaacgctg tcgctgatcg tcccctttct gcgggaaggg gacagatatt tagcatttga
     5281 tccgcttcat atctggatcg tacagcggac ggatcgatgc gacggcggga tattttttgc
     5341 cggcgatgtc gacttcccac tggcctttgt cgacaaagtc ctgatcgatg gcacagtcgg
     5401 cttcgatcat cgccaaaccg accgcgccac ccagcgtgtg accataggac gccgcgcgaa
     5461 tgtagccgag cgctcgttgg tttcggtaca cgacttcggc gtgaaacagg agcggatcag
     5521 gatctaatac ctgcacttgc agaagccgtt ttttgagcgg tccttcggcc tttttggcga
     5581 gcacagcggc tttgcccaaa aatccttccg gcttcttcag atcgacggca aaaccaagtc
     5641 cggcttcgag tacggaatcg gtgttgtcga tgtcatgtcc atagtcgcgg tagccctttt
     5701 ccattcgcag actcgcgagt gctttgagcc cagcgggacg gactcccaag tcacgtccgg
     5761 cctccatcag tcgatcgtag acatgcagcg cttgctcggt cggaatgtac agctcgtaac
     5821 ccagctcgcc cagatacgtg atgcggatgc acagcgcgcg ggcaaagccg atgtcgatgt
     5881 cccgcgccgt gcgaaagggg aaggcatcgt ttgacagatc caccgtcgtg acgcgctgca
     5941 gaagcgttcg cgaaagcggt ccctgcacgt tcagctgcgc atatccggac gtcacatcgg
     6001 tcaaaaacgc atggtgcgat ccaaagtgac gccgcatcca cgtttcggca tgccgatgcg
     6061 cggtatcgga agccaccacc cagaagcgtt cgtcgtcgag ctttgtcacc gtcaagtcgg
     6121 cttccagctt gcccgcttcg tttaaccact gggtgtacgt aatgacgccg cgtggaccgt
     6181 cgacttgatt ggcagaaagg tagttgagga gtcgccccgc atcgtgtccc tggaccaaaa
     6241 acttggccat aaaagacatg tccatcagga tcacgccttc gcgggtcgcg cggtgttccg
     6301 cttcccaata ggggaaccag ttttgccggc cccacgacag cttttcgact ctcggctcga
     6361 cgccaggtgg ggcgtaccag tcgggtcctt cccagccgct gacatcctta cagtacgcgc
     6421 cttgtgccac caatcggtcg tgaaaagccg accgcttgca gttgcgcgcc gtcatcatcg
     6481 agcgggtcgg atagtgacac tgatacacca ttcccagcga ttccaccgtg cgcacccgcc
     6541 ggtattctgg attcgattgg tagcggtgaa aacgatcgat gttcatgccg gtgatgtcga
     6601 catcggcgcg tccgtgcaaa atccagtgcg cgataagacg ccccattccg cccccggtga
     6661 tgattccgat cgagttgagt cccgccgcga caaagtagtt ttttagctcg ggcgcttcgc
     6721 caatgaccgg actcaaatcg gcggtgaagc tctccggtcc gcagaagaat tttttgaccc
     6781 cggtttcgag cgagatcgga acccgctcca tcgctttttg cagatacggt cccatccgat
     6841 cccaatcggg cgaaatttca ccaaaggaaa agtcggatgg gattccttct gtcttccagg
     6901 gcgcgcagac cggctcaaac agaccgacca ggagtccgcc tccttcttcg cggtagtacc
     6961 cataggagcc cggatcttca atcacgggcc agtccggact tatctgcgga attttttcgg
     7021 tgataaggta gtaatgttct gcggcttgca gcggaatgtt gacgcctgcc tcgtcgccga
     7081 gctgccgggc ccacatgccg gcgcagttta caacgtactc ggcttggatg tcgccgtagg
     7141 gagtccgaac cccagtcaca accccgcgct tgtgggtgat tccaatcgcg ggcacacctt
     7201 ccagcagctt ggccccttgc tgccgcgcgc ctttcgccag cgccatcgtg atgtcgaccg
     7261 gattgccgcg accgtcttcc tttacgtaaa aaccagccag gatgtcatcg acccgcgcca
     7321 gcggaaacag cgcctgcact tcgcgccccg acagctcatg cacatcgaca ccacagtggc
     7381 gattgaacgc tgcgacgcgc cggtattctt ccagtcgatc ggcatcggct gcgagctcga
     7441 taaaaccgac aggccgaaaa cccgtcgaca ggccggtctc ctcttcgagt cgggtgtaca
     7501 gatcgcgcga atatttgcgc agctctgtcg atgtttccga tgtcgatcca aacgtgacga
     7561 taagtcctgc ggcgtgccaa gtggttccgg atgtcagccg gtcgcgttcc agaagaacga
     7621 catcttttac acccatgtgg gccaggtgat aggcgacgga tgtaccgatg attccgccgc
     7681 cgatgatgac aacgcgcgca tgcgaaggaa tggaaggagg gctcatcatc gggcgaacat
     7741 acccgacttt aggcacgttc aatgatgcag atttgtcggt agaatggggg gcgatcaaaa
     7801 agctgaagcg ctcgttcaca gagcgtgggc acaagcgaaa ggccggtctg aaacaagcga
     7861 ttccaggggc cgcgtcgctc ggcgtgattg ataagcgcgt gaaagcggag cgattcgttg
     7921 ccgaaaaagc gcacccgcca tgctggttca agctgcgcaa acagactcgt ttggggaaac
     7981 ggaaactgca tgtgttcggt cagccactcg ggcggatcga tgttgtgttc gcggtgaaaa
     8041 gtcaggaagt cttcgtcgag cttctgcgcc agcgggccac agggaaaacc aaagatggcg
     8101 accttgcgcg caacccgcag cgtttccgaa atcacctgcg gacgcagatc gggcggaacg
     8161 tgttccagca catcggaaga caccacggcg gcaaaacttt cgtcggaaaa aggcaagtga
     8221 agcgccgatg cccgcaccgc cagcaggttg gccagtggct tttgcgcaaa cgttacatcg
     8281 cagccgacga tgttgtgcgg cagatagcgc gcaagtccaa atgcccccga tccaacctct
     8341 aggatcgaat cggtgtttcc gacgacgcgg cgcagcgcac ggacaatcgg ataatagcga
     8401 gcggcccagt ttaacaacga tgccctccga aaccgcccca agctaaacca aacggaaacg
     8461 tggaagggaa gcaaaaagcc gcttgacgcc tctcggaagc aaaagtaacg attcgcgacc
     8521 gatgaagctc cgggcaacgc tgcgtggcaa gacgtatcag tttcgcgacc tccgcgacgt
     8581 gatggcgaaa gccaatgaag aaaaatcagg cgaccgtctg gcgggggtgg cggctgccga
     8641 taccctggag cggattgcgg ccaagctggt gctgtcggaa attccgctgt ccgcgtttgt
     8701 cgaacatccg gcgattccgt atgaagatga cgcggtgacg cggctgattc tggatgagct
     8761 gaaccagccg gtctatcagt cggtcaaaaa ctggacggtg tcgcagctgc gcgagcatgt
     8821 gctgcgcacg gacgtgacag gtccggatct gttgcggctg ggtcggggtt tgtcatcgga
     8881 aatgatcgca gcgtgtgcga agctgatgtc gaacttggac ttggtctgtg cggcgcgcaa
     8941 aattcgcgtc gtcgttcacg ccaacaacac gctcggacag gaagggcggc tgggggtgcg
     9001 gctgcagccg aaccatccga acgactcgat cgatggaatt ttggcgtcgc tgcgtgatgg
     9061 tctgtcgttt ggttgtggtg atgcggtggt gggcataaac cctgtgaccg acaactggga
     9121 aatcacccgc aagatcctcg atgcgacgca cgactttttg gaagagtggc aggtgcccac
     9181 gcaaaactgc tgtctttcgc acgtcaccac gcagatgaaa gcgctgcaaa aaggcgccaa
     9241 gctggggctt atgttccagt cgctgtgtgg aacagagtca ggactgcgcg cgttcggaac
     9301 ggacatcggc atgctgcgcg aagcctggga catgacccgc aagctcggca cagcgacggg
     9361 gccaaacgtg atgtattttg aaaccgggca gggcagtgcg ctgtccgcca acgcccatca
     9421 cggtgtcgat cagctgacct gcgaagcgcg caactacgga atcgcgcgct actaccagcc
     9481 gtttttggtc aacaccgtcg tcggtttcat cggtccagaa tatctgttcg atgcgcgcca
     9541 gatcacgcgg gccggtctgg aagatcactt catgggcaag tttcacggcc tgccgatggg
     9601 ctgcgatgcg tgctacacaa accacgcgga cgttgaccac aacgatctcg aaaacttgga
     9661 aattctgctc gccaacgcgg gctgcaactt ctttatgggt ctgccgatgg gggacgacat
     9721 catgctcaac taccagagca cgtcgtttca cgatgatgcg acgatgcgtg aagtgctggg
     9781 actgcgtccg gcccccgagt ttgaagcctg gatggagcgc gtcggtctgt gggaaaacgg
     9841 acgcttgtcg gcgcgcgccg gagacgccac cctgctgacc taagatgaat gacattcctg
     9901 ttaccactgc ggtgcggttt ctgcgcgcgc acaagatcga tttcaagccg cacctgtacg
     9961 actgggaaga acacggcggg actgcgcact ctgccaagtc gctcggcgtc gatgaacatc
    10021 aggtgataaa gacgctggtg tttgaaggcg acggcaaaaa gccgctcatc gtgctgatgc
    10081 atggcgaccg cgaggtatcg accaaaaacc ttgcgcgctt tttgggctgc aagaccgtgc
    10141 agccgtgtac gccgcagaat gtgacgcgct acaccggata tcaggttggt gggtgtagtc
    10201 cgtttggaac ccgccagagt ctgctggtgt acgtcgagca gtccattttg acgctgccgc
    10261 ggatgttcat aaacggtggc aagcgcggct ttttggtcga gctttttccg acggcgcttt
    10321 cgcatctttc gccgacccag gtcacggtcg gcatctaagt cctgccgatg cgaacacagg
    10381 ctgccgcttt cgccacggga tcggtctttg accgtgccga ttgacggagg cccggcaact
    10441 gccgcaccct tttgtgatag caaaaaagcc aaggagagcg catgcgaacc gccaaagagc
    10501 ttctgtcaaa ctacgccgag tatcaccgcg atcgtcgcaa catcgcgacc cactttgtcg
    10561 gcattccgat gattgtcttt tcggttgcgg ttctgctttc gcggctgcac attcccttgg
    10621 gtccacagtg gctgtcagca gcgacggtga cgtgggtgct gaccacggtg ttttatctga
    10681 gccggcatct tgttttgggg ggcgcaacat cgttggcata cgggcttgcg atctggtttg
    10741 cgcagggcta tgcaaacggt ccgttttcca cctggatttc cgtcggtgtc ggctgtttcg
    10801 tagtcggctg gatcattcaa tttattggcc actactatga aggtcgcaag ccagcctttg
    10861 tcgatgatct ggtcggtttc ctgatcggtc cgatgtttgt tgtcgccgag acgctgtttg
    10921 cgatgggagt ctgtctggaa ctgcacgagc acatcgttgc aaccgccgga ccgacgcgaa
    10981 ttcgtgacat gagccgagcc taagcgatgt cagggcacag cagtccggaa cccaaagtct
    11041 cgccggtcga gctgtttttc gatctggtgt tcgtctacgc gattacgcaa gtgacgacgc
    11101 tgcttctttc ggatctgtcg ccacttggtt ttgttcgcag tggtctggct ctgtttatga
    11161 tctggtgggc ctggtcgctt tacacctgga tggcgaacgc gattggaccg tcgggacgag
    11221 cgacccgcgc cggaatcctg gtggcgacgg tgccgacgtt catgatggcg tttacgctgc
    11281 cgggtctgtt ccgcgacacg gcgctgtggt ttggcggatc gtactttgtt gtgcgcgagc
    11341 tggggctttt gctctactgg atttttctgt cgcacgatcg ggagcaccga tccggactgc
    11401 ggctgtttgt tccgtatgca tcgcttgcgc cgatgctggt gctggctggc ggttttgttt
    11461 cggcggaaac gcgcacgctg ctgtttgcgg cggccatggg aatcgacgtt ctgggggcgc
    11521 tcagctctgg gcgcggtctg ttccggatct ctgcggcgca ctttgcagag cgctacggac
    11581 tcatcgtgat catcgcgatg ggggaatcgg tcatcgccat aggaatcgga gcggcacagg
    11641 cgcactgcga tgcgtggctt ttgggcggaa tcacggtgat gctgctggga gtcatcgcgc
    11701 tgttttggtc gtactttgac tgggtttctg aagcggcgga acatcgcctg gtccatgcca
    11761 aagaagccga cagaggccgc ttggcgcgcg acatgtatac gttttttcac ttcccgattg
    11821 tggccggaac gatcctgtat gccgtcgctg cgaaaaaaat tctggcacat gcccacgatc
    11881 cgctgtctct gtccgggcgg ttttgccttt cggcaggaat cggtctgttt ctgctcggct
    11941 ttgtactggg cagccggcgg gtcggtggac ttgtgatgta tgaacggctg ctggcgattt
    12001 cgctgatcgc ggcgctgtct gctgtgccgc tcgcgattcc ggggcttggc ttggctgcgc
    12061 ttgtgctttt tgtcctggcc tgcgcgcttg cactggaaaa ccgccgtcgc aagcgtcatc
    12121 ccgcccactg accgcaaaac ttctttcacg ggatcttttc ttgcgccaaa gaccggagta
    12181 cgttccggct ctgtttccgt ttttcgtccc cccgaaacga aaggagtcct ttcatgaacg
    12241 cacgcaaaca cctgtctcga tttttggctc tgtcggtgat gtcgcttctg atgctcattt
    12301 ccgcgagcgc ccaggcgacc aactacaccc tgtggattca tggccgaacc gcttcgacga
    12361 ccaagccggg caactacgcc gacttttcgt attggggtcc ggcgactgtc tctgctggcg
    12421 tcaacaaaaa ggccgtcaac tgggatggcg taagctacat aagctcccag aactatcgcg
    12481 tccgggacgc gctcgactgt tactgcaccg gcaccaactg gtgttacatc gcggtccaca
    12541 gcgctggcga ttaccagatt ggctacgcac tttcgatgta cggaggctct gcgcgataca
    12601 aaaagaacgc gacccccagc tcgaccggaa cgtgtggaaa caccgacggt acgacccaaa
    12661 ccggctggaa catcaagtgg gtccgcgtgg catccggtgc aagcggtggc agcgagctgg
    12721 cagatgccgg atcgtgggcc agctcggaac agctgacgca ggatctggtg acgaccacgg
    12781 cgcgtgccaa gtacaaccac aacaccacct atggcgtgac gttttataac tacgcgggcg
    12841 catcgggaac gctgtattcg gcgattctgc ccggacaaga tgacgaagcg gtggcctatc
    12901 acaccacggg cggtgtggcg ggaacggcgg gtgcgtcgtt tgggaatccc tccgactggt
    12961 ttagcaacga cctgacgctt gggacggcag cgtgcgaagg cggcgcagcg aaatggagct
    13021 atcactctgt ttcgtatcgc gatgatgccg aagcgctgaa ccactacgca tcaggaaact
    13081 gggccggaat cattggtccg gtgcgcaccg acgtcgcgaa atacgcgctg taaggaaggg
    13141 aggcaaagac gtggcacttg gtcgtaacaa aacgctgggc cttggggccg tgtttgcggt
    13201 gatcgtaggt gcggcgtatt ttctgtcgcg acctgagccg tctttgccct tgccggaagg
    13261 ttcgcctgcc agtccgtccg ctccgcctgt tttcgcggga cgtctgcaag cgccttccgg
    13321 caccctttcg ggtgtcgcca agccgcaacc tgaaagcaca gcagacgact ggccacagcg
    13381 tctgcgcgaa gcacaggaac ggcttggcaa ttacaagcgc tttgcgcggt atccgccggg
    13441 cagtcgcccc gcgcgcgaac atccagatca gatgacaccg tttcttccgg tcgttcgcca
    13501 aagtcccctg attctgcatg gcgaagccaa tgaagatgtc cggattcagc taggccaaga
    13561 tcgacgagaa cttgttgctg atgaagcggc aaagctgtgg gtgcgctgtg aagacagcca
    13621 gggaaaagtg ctgccgtgca aagtggcgca ggcgactgca atgccagcgc ctccgtcgga
    13681 cagcaagctt cctccggtgt ccctttcttt ttcagatgac ggacaaaatg gcgatgaaca
    13741 cgcgcaagac ggaaccctga ccacgtcgtt tcagccttcg cttttgggat tccgtagctt
    13801 tcacggaacg atgcgcgtgc aggttcagct cgcgcttggg aaagagcaag gctcggcgct
    13861 gtttgacttg ctctacacac cgacggcccc ggcgcgcttt accggaacgg tgaaggaatc
    13921 gcgagcggat ggttcgctcc agtttgtgct gggactctct gtccagaaag cggggcgtta
    13981 ttttgtcgtc gggcgcgtcg atgatcagaa ggaaaagcag attggctatt tgcagtggga
    14041 aggcgagctt gcgccgggca atcagaccgt ttcgttgacg gtgtttggca agctgatcta
    14101 cgacgaaaga cccgaatttc cgctgcgttt gcgtgatgtc gaaggctttt tgtttttgga
    14161 agacagcgcg ccggatcgga tgcacatgcc gcggcttatg ggagtggtcc acaagtcaca
    14221 ggtgtattcc gttgccgact ttgcggatac agagtgggat ggtgagatga agcgccgcta
    14281 cctcgatgag tattcaaaag atgtcgagcg agcggaaaag cgcgatccca accttccata
    14341 aacgtctttc ccgactacgt agtgggttgt gaaaaggctc gctgcatcgg aagatgttcc
    14401 gatggcagct tcgagcgacc aagaccccgc ggtggagatc acgtttggcc gtcaccgcgg
    14461 ggtgtttgac ggcgagcgcg cagtgatcac cgtccacggt gtcgtcacgg aatccgatgc
    14521 cgcgcagatg atggacttta tcttgcgctg ggatgcccgc gccggaaatc ttgggatggt
    14581 gctgcgtgcg ccagagtcgt tttcggtgac tccggaagcg cgcaagtact ttgtcaggag
    14641 ctcgcgggtg ggactgccgg cgattccgat ggcggtgatg ggaacctctg cgattgttcg
    14701 ggggatcttg acgctgctta tcaatgcgat gcggatcacg cttcagcgcg atgtgccggt
    14761 tcgcttctgt tcaacggacg aagaagcgct gagctggctt tccgatcaga tgaagctgcg
    14821 cgccgtgcag cttgccaaca tgaaagtcat cgcagcgacg ccaaagtcgt agttctgttt
    14881 ggcggtctgc gccgcgcaga acaaacgggg gacacgaaac cgactatcga ctggggcgac
    14941 gtttcaccga cgctgtgatg agccgtggtc gctgcaaaga cagccacttt accgcgtcgt
    15001 cttcaccgct cgcgaagtgc atcgccgatg tcgagttgcc tgccaggatt tgcgtcgccc
    15061 gaaacatcag cccaacaaac atacggatgg ccagcggggc tccggttgcg gcggtggcat
    15121 gaacgtcgat gcagcccttc ccccattcga cagcgtggcg ccgcgcatcc aggtcgattc
    15181 cggtggaatg ggtcagatca aggcgcagca gcaggtagcc gaagtgatca agcaccgatt
    15241 gatacacctc ggtgatctgc ttgctcttcg ccaagtccag gtggccgtcg gagtggacgt
    15301 ggaggatgtc atcatgaagc cgcagtcgca tcgaaccaat ctcggggggg atgagaggaa
    15361 gcggtcgctg ctgctccata ggagtatgtt cgcacaaagg ggggcgctac aggaagcgct
    15421 cccgacatcg gttgctggtg gttagccggt cggggcgagc ctggacagaa tggcggcgag
    15481 cttgtcggcg ggcagactgc ggagcactcc gatcagttga tcggaatcgt cagaagccgg
    15541 ctgcggattc ggatggggag tcgcttgctt ggccgtatct gccgtcgaca aatggctcga
    15601 tggcgtactc ccaagcagac tggacacttt gcttgctttg gcgttgtcga cgctgctttc
    15661 tgatgcatag tgccgcgctg tcatcgcaaa cgatccgtga cccagtgcgc gtgccaccgc
    15721 atcggcagtc gccccacctt ccaatgccag cgtcgcgtgc agaccacgca gcgagtgcgg
    15781 acagaccgct ggcacgcctg cgagctgaca gagccgccgc acctgcgacc agaaatagtt
    15841 ggtcggtggc actttgtcgc ctttgtaaaa caaaagtgcg ccgggcggag tcgtttcgat
    15901 cagcttgagc agaagcggcc ttagcacatc gggaaccttc agccgacggc gcgcattttt
    15961 ggtttttccc gacggaaccc acagcacccg gccttcatca tcgacatcgc gcgcgacccg
    16021 tgctgccact tcaccttgtc gcagtcccag gtgcagcatc aaaagcgccc cgagcgctgc
    16081 gacatccccg gcttgcgcaa gctccagcgc tttggcttca aaacgtcgtg cttcatcaat
    16141 gcgcagctgc ggcttgccga cccgcttgcg accaaccgga gtgaccgacg caaacggatt
    16201 gtggcggcac atctcattct ttatcgcaaa ctcgtacagc cttcgcgcca ggctcaatcg
    16261 accgtggtgt gtggccgtcg acagcggctt gccggtgcgc ggattgggag ccgtgaccaa
    16321 atcgcgatac agcttttcgg cgcgctcttt gttgacgctt gcgagcggcg tttcacttgg
    16381 caaccactca agctgcgctg cggtccacag cacactttcg ggcttgatgc cgcgtgtctc
    16441 ttgcaggttt tcggtaaact gtcggatggc ttcaccgacg gtgtagacat gctgcgcagc
    16501 gatgtcatcg cgcagccttg ccttggccag ctctgcttcg tcgtagctgc ggaaacagat
    16561 gctacgtact tcgccgtttt ccgaaatctt gagccgaaac ttgttgggcg catcctggta
    16621 ggggccccag atgttgagcg atcctgctct catttttcca tgatcgcaca ggcagaaagc
    16681 tgctgacaag cctttcaaac cactgggttt ttcacaaaac agagcgcaat cagcgacgat
    16741 gtcgccgcat gtggggaagt ggaacgtagc tgtaggtgga atgggaagag agccgcccaa
    16801 tatgaagtat ttctaaggat tcatatcggc catcgtgcgg cgaatttcga cggaaacggt
    16861 ctgggcatcg ggcactgccc ctggcttttc aacccgcacg gtcagcgctt cgatccgtcc
    16921 gcggtgcgcc aaaagaatcc gcagcgcaat ccgttccgcg acggtctcga tcaggttgtg
    16981 gtgccgctcg cgaatcactt ggcgcacaca ctcggccagc cggtcatagt cgagcgtgtc
    17041 ttcgagctgc tcgctgtgcg ccgccacgcg ggtgtctgtt tcgacgcaca ggtttaccaa
    17101 tagtcgctgc ggcgtgtcct tttcatttgg atagacaccg acgagaccaa aggctgtaag
    17161 cccttcgatg cagatccgat cgctcatccg ctactttcct ccgttttccg gcttgttcga
    17221 tttctttggc aacgcttccc gattcaccgg aaaagccacg gtacaacaat gtcaaaaggc
    17281 acggtcacat gagcacactc gcgatcactc tttctgacat cttggccgcc caggcgcgga
    17341 ttttggcgga cgtccatcac acgcccattc tgcgctgtca gacgctgtcg tcgctgtgtg
    17401 gctgcacgct cgggctcaaa gccgaacatc tgcagcgctc ggggtcgttc aagctgcggg
    17461 gcgcgctgaa ccgtctgcgc cagctatcgc ttgccgaaaa acagcgcggc gtggtggcgt
    17521 tttcgtcggg aaatcatgcg caaggcgtgg ccctggcggc gcagatttgc gatctgccgg
    17581 cggtgatcgt gatgccgagc gatgcgccaa aggtcaagct cgacgcgacg cgcggctatg
    17641 gcgccgaagt ggtgctgtat gaccgcaaaa cggaggaccg cgaagcgatt gcccgcaagc
    17701 tgtgcacaga gcgtggcgcg atcttggtgc ctccgtttga cgatccgcac atcatggccg
    17761 ggcagggcac cgtcggactg gagattctgt ccgatgttcc cgacgtcgac acgctgcttc
    17821 tcccaatcgg cggcggcggg ctgatttctg gcgttgcgat ggcggtcaag tcgctgcggc
    17881 ccagcgtgcg ggtgattggt gtcgaaccgg aaactgccga cgatgcgctc cgatcgcgac
    17941 aggccggtca ggtggtgcga atttcccagc cacagacgat tgccgacgga gtggcgaccc
    18001 aagcagtcgg gcagcttacc tttccggtga tgcaggcgct cgtcgacgac attgtgacgg
    18061 tcagcgaagc gcagattgcg gcggctttgg ggctggtcct tacgcgggcc aaacaggtgg
    18121 tggaaccgac ggcggcgctc ggggtggcgg cgcttctgtc tggtcggtgc cgcttgccgg
    18181 gacagaacgt ggtcgcgctt ctgtgcggtg gcaacctcga tctgtcgtcg ctgcctcgca
    18241 tccaagaact tgcaggtatt cggtaacaaa aactacattt tttacggttt acaatggggg
    18301 aatgctcgaa gcccttggcc caggccccct gtcgccggtg acggcccgct ttcgcgccct
    18361 ggtcagcgca ctgctatcgc cggcactgcc ggtgcgactc gacagccaga attcgaccga
    18421 agtgcagctg cggatcgagc gtcccggcaa accgcccgag tttgtggtca tcgtcgacgc
    18481 ggtcaaagaa gacgaacgct cgctccagct tcggctggcc cgtctgacgt ccgagagcgt
    18541 aaacgatcaa catgtcgtgg tgctgtcgaa ccaaggctgg ctgcccgacg tgttgaccaa
    18601 agcggcagcg acgtatggat cgcggctgaa gctgtatcag gtcgattccg aaggcaagtg
    18661 tctgctcggc agtccaggcg gagcgcttgc ggcggcgctc aaagcgcaaa aagagctgcc
    18721 accgtcgcag gaaaccgaag aagagtttgc caagcgaatc agcgaagccg agtcggaaaa
    18781 gcagcgccag atcgatcgtt tcgaagaacg gctgacggcg cagagtccgg tggcgacgtt
    18841 tgtcctggcg ggacttttgg cgttgctcta cgggctgttt cgtttgtgga cgcctgccga
    18901 cggggcgctg tggacccagc ttcagcttgg aatctgcgtg ccgcgctttg tatcagacgg
    18961 cgagctgtgg cggcttgtga cgggcggact tttgcacgat cagctgctcg tcgtgggact
    19021 gaacatcgcg gtgcttttgt cgcttggata tcagacggaa cggctgattg ggacggggcg
    19081 gtttttggtg ctggttgtgg ccagcttgct tgcggcggcg ctctttgcgg tgattccgac
    19141 gccgcagctt ttgattccgc gcaaatacgg tttttccccg gcgctgttcg gcatcatcgg
    19201 tgggctgtcc gggctgtcga tcttgccgcg cagcctgccg ccggaaacgg tggcgcgcgt
    19261 gcgcaaagtt gcactcgcgg gcgtgattcc gggcgcgatt ctggcggtgt taagcggcga
    19321 gcggggcacg cttttggcgg gctttgcggt cgggctgatt ttggtcgggt cggggctttt
    19381 gccgtcgttg cgactgtctg atccagatcc accgagcggc gtgacccggc tcgcccagca
    19441 agcgatggtg gggctttcgg cgcttttgct tgtcggcagt ctggcggtgg catttgtcgt
    19501 cggcaagccg tggtcgcaag aacaaagcgc gcgtgagcgc ttcagtcgga cgatgctggc
    19561 gcaggcgctt ggcaacggca aaagcggtcc agcgggacag tcgggatcgg caacagcgac
    19621 gggtggggcg acaccagaag ccgacatgac gccgcagctc cagtcgctgc cgggcgtcgg
    19681 aattggcctt gtgctgccga agctgctcgg tgatccgcag cttcagggcg aaggcgcgca
    19741 gcagacgatt cgttttggcg acctgactgg aaaacttcag cagctcgatg tggcggtgcg
    19801 aaggcacgag gctccgttca aaaaacgtgc gcaggtgctg gtcgcattcg acaacgcggt
    19861 ggcgctggtg aaaaacgaca agcagcgcga ggtcaaagcg gggcgtctca cactcgtcgg
    19921 cgagccacgc agctttgaag ttggcgacct gccggcgctg gaaatgacgt ttaacggcgt
    19981 cgaggggcaa aaagcgcgca ccgccgtgtt cgtcaacaag caggcgctca tcgtgctgtc
    20041 gtatctgtat tcggatctgc tgccgccgaa ccttcagctt gatctgggca aagtgctttc
    20101 gtcggtcaaa gacgagtttg ccgattctcc cgtcgagcag gccaaaggca aaaagaagaa
    20161 aaaagccaaa aagcggcgct agcgactgcc cggcggcaaa agcggtcgaa aaacgcccga
    20221 tcgcagctta gtatcgccgg ccatggagct ttcgtcgtcg tcccaccgct ttgcggtcta
    20281 tccatcgctt gcaggcaagg tcgcgctggt cacgggtgca tccagtggca tcggggccgc
    20341 ctgtgccgag gcactcgcgc agaatggctg ccggttgatt gtgcttgctc gtcgggaaga
    20401 acggctccgg tcgctggcgg accggctgac ttcgctcggg gcccctgacg tgtggccggt
    20461 ggtctgtgat gtcgccgacc gtccacaggt gatggcgcag ctggcggcgc tgccaagtcg
    20521 actggctgct gtcgacatcg tgattcacgc ggcggggctt gggcgtgggc gcgacaagct
    20581 gcacgaggga aatccggcgg aatgggacga agtgctcgat tgcaacctca aaggggtgct
    20641 caacgtgacc catgcggtgc tgccacagat ggtggcgcgc gacgctgggc acatcgtcca
    20701 catcggctcg gtcgccggca gccagcctta ccagggcggg gcggtctact gcgccagcaa
    20761 agccgcgatt cgcatgctca accagtgtct aaagcacgac cttctgggca ctcgcattcg
    20821 cgtaagcagc gtcgatcccg gcatggtcga aaccgagttt tcgatcgtgc gcttccgcgg
    20881 cgacatcgat cgcgcccgcg ctgtctacga aggcatgacg ccgatgaccg ccgccgatgt
    20941 cgccgagtcc gtgctgttct gtctgtcgcg ccccgcccac gtcaacataa gtgaagtcat
    21001 gatgctggcg accgaccaag ccgccgcaac caccgttcac cgtcgccccc gctagcctca
    21061 cgcttgctcg acagatgtgc ccacttttcg cgaccttagc cgcatagggt tgcaaaatcg
    21121 atattttatg tggaaaaatc caaagcgcgg aaactgcatt atcaagcaaa gatggtgcgc
    21181 tggtgaacgc cacttgctag atcgatggtg caatttcctg cgctgtggtt gctgagtgtg
    21241 ggaacgatat ccctgtcgga ccattgagaa cttcgactca aggaaaatca acaagatggc
    21301 gactcagatc attgatgtag atagcgaaga caaattcccg cgaattcatt tgatcgatca
    21361 tactgggcct ggcggatatg gtggatttat cgtattaaca gggtctgata ttacgaattt
    21421 caaaggctat ttgaagaaat attctggcca tcggctggtt agtaaatatg caggaattca
    21481 tatttactgg ggaacctata ctcgtgcaaa aatcggcaaa gatgaaattt atgattccat
    21541 gaatcttttg catcagggct ggggggtcaa gacgatcagc gccgtcagca cgatacagtc
    21601 attcgcccaa gcaaccaccg tgacccagtt cgcgcaaaaa ggtgcaatga aggtcatccc
    21661 ttatttgatt ccttcgggcg atcttcccag tcctcgtgat ttgctcgctg agaaaaatcg
    21721 cccctataaa cggggtgata ccaagaaaca catatttagt gatttcatgt ctcttcttgg
    21781 ccgagcagga tttgcatcac gctatttctt gtcaatttca taccgggacg gaagctctgg
    21841 tcatgccggt ggattgcgcg gtcaagaaaa aatcgcaatg cttggatatg tcgatgcaaa
    21901 tacatcgatt aactttgaac aaatcgccca gctatatttt taaatagttg gcgaatgttc
    21961 ccgaattagg cgtatcccca gcggcggcgc agcgtccagt tgagtccaaa acaggtgacg
    22021 gcaatcgcca gaaccagtgg attgtcccac agcggaatgt cgcggtggtc gcggacgtgc
    22081 tcgatgtgcg gcgggtgaaa cgaaagcccc atcagtgaat cgccgggttt tttgtgatcg
    22141 ccgcccgtcg tttcgctgac cagcttcagg agtcggtcgt cggcttcggg cgcttccagc
    22201 tcgcggccaa ttccgcggac caagaacacg tcgctgtcgt cggtcggacg cccggcaatg
    22261 acggcgcggg cgatgacccg gtagccgcct tcggggagct gcggccactc gatgctgccg
    22321 acgccttctt catcggtgtg cagggtcttc gacatcacgc tttcggccag tggttccgac
    22381 gtggcgcccg attccgtccc ggcgatgcgc tggagcgaca gcgagacttc gacttttccg
    22441 acgggctgat agtccggtcc aagcgcccgc acctcgacgc gcaccggctg accccgacgc
    22501 agctcggttt cggcgacctc gacgcgcaaa agccgcagcg acggatcgcg aatcagccac
    22561 cgaatcgcct gatcaaaaaa gcgctgatac gtcgtgctgc gggtgtcgcc ttccgctttc
    22621 gccgcggctt tgccggtctg tggcggcgca aacgcccact tccacgtcga gtccgcttgc
    22681 agtgccagcg tccgcccttt gccaagctcg gccaccgaca aaaccggcat cggattgccg
    22741 tcttggtctt tgagccgcgg atgcgaaagc agcaccgtcg cttgctgccg ggctttttgc
    22801 accagattca gaccgtcgag cggcggcagc tgttcccagc gctgggcatt ttccttcaca
    22861 tcgaggtgca gcgccgtcac gacgtggttg cgtccggctg gcgtcagctt gggcacaaac
    22921 ggatcggcat caatcagccg cgacgacggt gcgccggata gctcatcggg cggcagcacc
    22981 accggcagga tctgcgacac ctcggacccg tagtaaccac ccgacgaaaa cgacagatcg
    23041 ccgcccagca ttgcgacggc accaccgcct tcgacgaagg gcacgatggc tggctgcaaa
    23101 aactgcgagc agtacgcgct gtagttaaag ttttgcaaaa tcaacagatc gaagctgcgc
    23161 agctggtctt ggaacagctc ctgacacggg aaaggaatca gcgacgtttc ttccgacgga
    23221 gcaaactcga tgtcgctcgg tgagcgaagg ataaaaaacg ccaccaggtc gatgttggga
    23281 tcgctcttga gcgcggcgcg caaaaaacgg gcatcccacg acgggcgacc cgagacgtgg
    23341 acgacgcgga ttttttcgcg caccactttg aggacgaacg cccgccggtt gttttccgaa
    23401 atcgcttcgc cgggctgcac cggcacgctg atgtcgtaca ggaacttgcc gacgcggtcg
    23461 ggggtaaacg ggaagaccac ccggtagtcg cgctgctcgg gctggacttc gatgtcgacg
    23521 gtcaggacgg gcaccccgtc gcgacgcagt gtcaccggca ggtgtcgtcc ggcaaagccc
    23581 gccgctttgg ccccaaacac ttgcagccgc gcttcgactt cgacggcggt tcgggcaaac
    23641 gcaaactggt cggcgagcac tcgggtcagc gcaatgtcac gaagtcccgg tttgccgacg
    23701 gcgaccgtgt ggaccggcac atccagcgca cgcagaaatt cctgggtatc ggcgtcgaag
    23761 ctaagctgtt cggggtttgg gaaagacagc ccgctgctga gtcgtccatt gtcgagcccg
    23821 tcggaaaaca tcaccacgcc ggccaaatcg cggccttcgt accgctgtcg cactgccgac
    23881 agcgcttcac gaattcgcgt cgcgtcggcg cgcaggttgg ccgggtcggg atgggtcacg
    23941 ctgtcccacg tcgccgactg gagccgctcg ccgaagctgt aaaactcgac cttgtggtct
    24001 ttttgccact gcccgagcgc gcccagctgg ctgcgcaagt ggttggccgc gcgggccgct
    24061 cgcgtttcgc cgccgccttc ttcggccagc cgcatcgatt ccgacgaatc gaccagcacc
    24121 gcgatgtgat tgggctgcag gctgacgttg cgcaggcgaa tcgtcggctg aaaaaacaac
    24181 agcagcgtcg ccaagagtcc gacggtgcgc agcgacaaaa gcagaagcct tcgccgcggc
    24241 tgttccccgc gcagcgcccg caccgccagc ccgagcacga cgacgacaat cagaagcgcg
    24301 acaatgcggc ccgacaggct gcgcggtgcc aaaagtgtca gcttccactc gttccagtcg
    24361 ccgccgagca ggttcatggt gtcaccggcg caggagtgat gctgggaagc tgcggctgcc
    24421 aggcccgtcg gcgcatgata aacggcacat ggacctgatc cgccttgtag tcgagacaca
    24481 gcgaatacaa aaccaggttt atccccagac gcagcgcctg ttcgcgctgg acttctccgc
    24541 cggggaccac atcgtgcagc cactgaccgt agcgatcgcg ggcccacgcc ccacccaggt
    24601 cattttggct gtataccact gctgcccgtc cgcgaagctg caccgcttcg acgtagggca
    24661 gggcgatgat gcgaccaatc gggacgcgca aaagataaaa cgaccggaac aggacgtgat
    24721 cgtcgggcag ccggccaagc ggtgtctgcg gaaacagctt ggtcagcagc gcgcgcaccg
    24781 actgatcgaa cgctccgccg gctcgaccct cggcgctgtc gatcagcagc atgcctccaa
    24841 gctgaaggtg ccgccgcagg ttggccagct catcgtcgct cggcatggga aacgcgcgat
    24901 cgccggccag gtacaaaagc ggatgccggt gcagcgacgc gacatcgccc agatggacga
    24961 tctgggaatc cgagttcact tcgacggacg ttcgcttttc cagttcccac aaaagccggg
    25021 acagggcttc gggccgcggg atcgcctgcg ccgtcggcag cccggtcagc tgaatctgcc
    25081 caagctctag cttgtcctcg ccgccaaaac ccaaggcgag ccgcggcccg gcgacaaaac
    25141 acgccagaag cagcagaaac agtcccccaa gttgtgaacg taaacgcacg agcgacggcc
    25201 tatttataac ggatcgcaag cccgccgtca ccgccagagc cttgcaaaac aacaaacgcg
    25261 gttgaaaacc atacggtgca tgtggcggtg attgggcgag acgagccctg tcagtgcagg
    25321 gaagggcatt tgcagcgcag ggaagggcat ttgcagcgca gggaagggca tttgcagcgc
    25381 agggaagggc a
//
LOCUS       DAORGN010000053        24726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_793_length_24726_cov_5.668761, whole genome shotgun sequence.
ACCESSION   DAORGN010000053 DAORGN010000000
VERSION     DAORGN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 24726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24726
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_793_length_24726_cov_5.668761"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PKL17_08150"
     CDS             complement(<1..968)
                     /locus_tag="PKL17_08150"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNK44739.1"
                     /translation="MNSQLDFFSLTPDAVLGAVEQALDPTGKIRATGRCFVHASMENR
                     VYEIELEEPLAGVGGKVIAKFYRPGRWSHAALVQEHDFLRELSEAEVPVVAPIAMAGA
                     EGKTLHKTSFGVFFSLFPKVGGRVKEELQEAELLQIGRLLARLHTVGAAHPAADRPRL
                     TPAEYLLPALHSLLQSGLVDMQLQSRYERAARTLFRMAEPLWQGLTLHRVHGDCHGGN
                     LLWQPSGPVFLDFDDLMMAPAVQDLWMVVRGRDAEATQQREIIIEGYEQIRDFDRRTL
                     RMIEALRGIRMIHYASWVAKRYADPIFVRTFPEFPTYAHWADETATL"
     gene            complement(1007..1606)
                     /locus_tag="PKL17_08155"
     CDS             complement(1007..1606)
                     /locus_tag="PKL17_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK44740.1"
                     /translation="MMSIWATANLFGLLVAAAVGLTEPELEAALRGDTPTRMESFTTE
                     TGKASGRGVGAIVIDRPMAEVWSVVSHFPDKAEYMPRLEKVEVLEATKERLRAKMTVD
                     AAVSTARYTAIFSISQAQHTIDFQLDKSASDNTIKDTVGGYALFEVSTEKTLLVYRTW
                     VDTGRAIPRFVADYMSRKSIPNLLHAIKKRVESRGLYRK"
     gene            1716..2681
                     /locus_tag="PKL17_08160"
     CDS             1716..2681
                     /locus_tag="PKL17_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HNK44741.1"
                     /translation="MRRLLRILVISACAGTVFSPQSGHGYVRTRTKVSCRPLRWAQTC
                     IFLQPDAELLRDDLPADVTLQAIQSAIAAWNDRLAPSSFLQLHYLPPTDSREASQTDR
                     LGWIKFRFGSWCRPPKDSSSSPVCFDPSATAVTTVSFLNKPTEPDLDGQIVDTDIDLN
                     AVGYRFVDGSKPLSTGDRRSPVDLWNVLTHELGHVMGLDHTCLIDGGSSGCMTDHKGA
                     QVVTCATLDGQRFSSQDAETAYEAAMYPTTTAGDYSRRIPTADDLAGVITTHPASADP
                     QVCSLPSVASGCTVSAQVQFGNPPFWAALGGLSVLLALSLRLRRR"
     gene            2770..3399
                     /locus_tag="PKL17_08165"
     CDS             2770..3399
                     /locus_tag="PKL17_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNK44742.1"
                     /translation="MIKLYGMTLSAHTRKVQSALIELGLPYEMITVDLTKGEQHKPEY
                     LALNPGNKTPTLVDGDTVVIESNAILLYLAETYGKGKLIAESGKDRWQTVQWLVWTAS
                     EGHGPLSRPWYVKVLFPMMGQPLDEAGLAKSHEDARVPLKVMDSVLAGQPYFGGSNFS
                     VADIALAESVHLSQWGGLDTSSFPNVTRWFHDVTSRDAYKQSRFQHSAS"
     gene            3583..4059
                     /locus_tag="PKL17_08170"
     CDS             3583..4059
                     /locus_tag="PKL17_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNK44743.1"
                     /translation="MKRNHGACVGLLACALGLLGVGTANAEEIGSVSTVFKMVGPNDK
                     IVIEAFDDPKVDGVTCYLSRAKKGGIKGGLGVATDTSDASIACRQVGPIKIKEPFPDG
                     EEVFKKDTSLLFKTMQVVRFLDRKRDVLIYLVYSDKLIDGSPKNSVTAVPLRNPTP"
     gene            complement(4063..5139)
                     /locus_tag="PKL17_08175"
     CDS             complement(4063..5139)
                     /locus_tag="PKL17_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNK44744.1"
                     /translation="MMIRFLIATLVSSSLFLSAGCGSDPQQSPDQPPPNQLSDPATCG
                     STQLPRVIVRGNQLGVVCHGSEVPVRLKGINRSGLQHKSSLGNAGFPSNPQDELHRFR
                     SEWNAVAVRLPIAQDHYMGSASYRDDIGNIVSATQSEGQYLILEVHGYSYNLNPPLPD
                     GQTSVLWDQLARRFGKESHVLFDLWNEPRASSWDVWKTQAESLIKTIRSAGAQDTVVV
                     VGGLDYAYDLSALADANNRISSGLGPIIYATHPYPFKGTPPHVEPDWDRYFGNVSRDV
                     PVILGEYGVDEMIMDAQAARDWMARLHRYIDGKGLSALAWSVGDMPHLVLGTNGSGVV
                     LPSNPPDPSRPTDPFGIAVKAWMK"
     gene            complement(5149..7593)
                     /locus_tag="PKL17_08180"
     CDS             complement(5149..7593)
                     /locus_tag="PKL17_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44745.1"
                     /translation="MLTGQLGPYQLVKKIAVGGMAEIYRAIVPDTEPLRQVAIKVIHP
                     NNSEDPDFVRMLLDEARLAVRLKHPNIVTTYDLGKDKEQYYLVMELVEGVDLFRLEQR
                     ATDLRLSIPVPLCAYIARELARGLHFAHELADPAGKPLHVVHRDVSPQNVLLSYEGAV
                     KLTDFGIAKAEGRSEQTQLGIIKGKYYYMSPEQAAGEPLDRRTDIFACGILLYEMLAG
                     EMLYFDQNIERLLDKVRKAEVPQIVRRRPDTPQELERIMMRALRRKPDDRYKTAEDLA
                     QALDGFLRRHAPTFQASELGKFVEKVQTTQVKKRGADEPTAALDLSLAAKQSMSAPDV
                     DEEDADRSPSPTMGIDAAQLEQMRALGIKDDNSLLFQSGNLKALLERKAPGRNDEDDA
                     DDARTGTDRGLRPSDHSDVTTHRRISGRLVEAAPPKAVFPAITADGEVFGLLTEGADL
                     LPRPTELGQVGAAIRRDGSGSLYPKNQADGLGDSVTAVAPVSVLPPGVERSKRTRRPS
                     SQSSGEPIRETQRPRQSEAKLELLQSDVSYDDGGSAPGRPAVELAQSDDFAPLGSLAK
                     QEQKKPPPKAGQSSGERNAVSIVAEPSGQIDIISTELTHEALSAPADLRAPQPDPEAS
                     VEVGPSPNRWLFPLAGVSAALLSAALTWFLVIGPQRSQRSLSPDDLAVQDLAPLSDLA
                     DKPPASDAGSETTKPNGEAGSPVATTDKSKPSAVPAEDKQKPSAGQVLVRSEPPGASV
                     RIDKEEVGVTPLLLLGRTPGEDFKIELKLEGYKKGRKRVKWKDKDRLEIKVKLQSESA
                     ESEASSDEKTEEKETK"
     gene            complement(7693..8655)
                     /locus_tag="PKL17_08185"
     CDS             complement(7693..8655)
                     /locus_tag="PKL17_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44746.1"
                     /translation="MDSFQQPAASGQRILIVTEDPVLRQRLSQVVRTAGVQPVAVAGV
                     HEIAGPQAPHRLVIAAWDGKLDWMKTLCRKLRDSAQLIAMVPQRSLAEQVALLSDSHC
                     NHVLTADERGMFLLQVTIGKFVSGELFGLERYLPPGTPIQLTRLRDFQGRQNAIDEVL
                     SFADKSGVRRQVRASIGQVCEELLMNALYDAPVDANGNPLFHHVEVKERVDKLSPRPV
                     SIRYAVADDQFAVSVRDRFGRLEKATILRYIEKCLKSSDQIDRKTYGAGLGLYLIVNA
                     ATQYVVNVAPGMATEVVCTFDRGTRQPLRALSMFVHPGNPTSKE"
     gene            complement(8746..11355)
                     /locus_tag="PKL17_08190"
     CDS             complement(8746..11355)
                     /locus_tag="PKL17_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44747.1"
                     /translation="MGRVRLQADFLDDKDQASDCPDFLVGDDLVAQKTFAITKPQRLR
                     LRVGAEAPIVALYLLFAGEGMVDTWNSWRRSPRPPRENDLRLDANGSPCPCRRETLPL
                     PLTLIPPQTRAVFPATILAFFPDEPMAPESELVIWLPAGPHQLARVIVSTEKLALQAA
                     PALPLPAAPSQPKSSSQLPKHGDQLTETLQKRTSDPTVADLHQLHLPFRGGALLGQVE
                     GPIAYQLWDGGIVLRQWGEGTDRALYRLATDWLQTNEMHGQQRLQRGYLPIAEVELSA
                     GDKTLTQRVFVDHEHCLRARYTLRGLTKERIEDEVWQCGLSGCENDPKALAQRISRQP
                     GDAQELTILLSQRTGTRSLERVDGGVRLDVTIPLAEHSPLRSFEEAEQFLSADCEAYL
                     QRGAELSFPDPLFAKLWRALLLHNRLFVQGGVMRYGLFPGVYDGGVFGVEEGWNIVAL
                     SQYGHHDEAARVLERTFFDPQFLAKEGQHHQYRNGLALTYAADVYALSQDRTLLARLW
                     PQIAESAEWIAASFRSTQQLVDGNRPIHFGLLPKHTYGGDLTTPAFGLYGSSACWRGL
                     RDAGRLAKITGDGRADSWLAQAAETRRNLLLAAERSFQHNGTPPYLPFRTDETGKTPS
                     AGDYHQLFASLILETALFGWHGRFSHEITDYLEQTGRQVLQVARFDDWFGRLGVDAEY
                     SRGTQLCALHRRDFSRFYLGLLGQVALSCDPYTFVSPETAIVLFDDTEWRDRMKALSQ
                     QPCRFDSDPCSAGTAVMLQYLRFLVVSEERDEDDLPTGTVLVGAALPKSYFAPGQHFA
                     AHKLPTLFGPISVDCESSERETIYRIAYDQAFTVELFYFDRAGQHRTHKTQLPVPAQT
                     TRPHTPTVVRLFR"
     gene            complement(11398..12333)
                     /locus_tag="PKL17_08195"
     CDS             complement(11398..12333)
                     /locus_tag="PKL17_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44748.1"
                     /translation="MRVWIGLLSLLLLVVGSSGTIDIAAARRPPKSATPAVAPAPPVA
                     PSAAPAPSAAPATPAAAAAAPSAPKTAIKVICATEGAELSIDGELIGQTPFKDIVPLS
                     PGEHTIRVSRLGFTPYIDVIKAKNGQTSKIEVDLTPISGVLGITSTSVKDGPAVRVFI
                     DDKFFGKAPLETEVPLGNHQVRVERPGYYSETFAIKAEPGKRIDKDVELKPLPADQNP
                     YLVKQPQVKWYQKWWVWTIVAVGVVVVATAIIVPVVLSQQTICKDVDVCTTIQAAPLR
                     IQPYSLTQPIENPRFVQPIGAPQPTQLVPSLTLRF"
     gene            complement(12414..14702)
                     /locus_tag="PKL17_08200"
     CDS             complement(12414..14702)
                     /locus_tag="PKL17_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44749.1"
                     /translation="MSDQPESGEDLEPAHSSFRRAPKGGDVLDSIYRLDAPLGKGGVG
                     VVYRAWHLHLQRPCAIKFLHPQLVSNPELRTRFRREAQSAFQLGHPHIVAITDFRDDG
                     TNWPYLVMELVQGQSLRDRLEKGPLDPQLSVRLMAELADALVVAHRRGVIHRDLKPEN
                     LLLANVENPPPGEPALTLKVLDFGLSKMLDGVEITGSGRLVGSPSYMSPEQARGDSHL
                     VDARSDIWSVGVLLYECLTAEKLFSCDDFEQKRQMIMAAKLPPLPFTERGLPTMIEKI
                     ITRCCQQKPEHRYQSATSLLHSLNAVYPKPQPRIDTLPPGTKLQGGVLVLGLPPSGVN
                     PTGSQQHEALVAAPSAGESQSGPEIVIVPAVVKSMLPDSSTASVIKNGELVQGGAPKP
                     GLRKSHVAIAAVAVSAALGFTSLAGYTLWQSQQKTPIPNPNAATTVPQDPLIKVSGVT
                     DPKPADPTGVASSPDAKAAPPAVTPDLGVAALPKDSPSPVLPTGDEKDEAKDAGAQGQ
                     TGIAAATSPRVHTPPNLPLRGPRAKAIGEPPTHPLRFGPPRHANLAMLGLPAPASAVP
                     FAGPAPDAKDGKESASAAGNPVAAEKPAAKEATSKDAATAAEKTAAPAKDGAATSASK
                     EAVVKEATSKDTASRDGTAKDDKDGEPAKAEKPARKATPVADAKNEPVTTQEAAMAAN
                     AILRKATPQVGRCYPAEVVPPTKISVELTVAKNGKITEASVEGAGDQTACVRNAVKLI
                     RLGAISDADSYNLQYDFVNIRR"
     gene            14860..15093
                     /locus_tag="PKL17_08205"
     CDS             14860..15093
                     /locus_tag="PKL17_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44750.1"
                     /translation="MSTGSNRDGDGDRIPPHELPFPASLCHLCQAPRYIRSGKGSVFV
                     LCPLLPEKYPRQPVRTCALFSPRADDGSSSSDR"
     gene            15210..16034
                     /locus_tag="PKL17_08210"
     CDS             15210..16034
                     /locus_tag="PKL17_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44751.1"
                     /translation="MRGDKSRMQSQRLLLAASAASLLFLAQSAAHAEVHLAIGSRFEP
                     YRYTMAQFPNAATAAGDLAVANQSFQTTSLSPYFGAFFSQRYGLVLGLDLGWAKQNTE
                     KQMGANPALTATDSYLQFGLGLGFKMYLREPRKDRIAPYAYVDVFKYFASVSTTNTAV
                     TGDQASAQADTSSPVGGTLAIGAEYFLGTGFSIGAEVFGLRVSHVGAEYTDATMTRRS
                     VGFTELSMYSGLTLNYRFAVGSGSKSDEPEEDRRRTSTPSAPPPPSVPPPTPEAID"
     gene            complement(16056..16499)
                     /locus_tag="PKL17_08215"
     CDS             complement(16056..16499)
                     /locus_tag="PKL17_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44752.1"
                     /translation="MKDRLTKKQLSVSMYDRGMNVVAIAKLLRCEPSYVANALIDQGR
                     VPAYVDLYTSTGPQNEDAKELEGVLKFTDAASARDSVAKIEKLWQRFSAERNRRGMHQ
                     CEVVALIGRNRAIGIGKHVEAQVFADWLLERLRSPATKNPSPKAA"
     gene            16730..17152
                     /locus_tag="PKL17_08220"
     CDS             16730..17152
                     /locus_tag="PKL17_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal family protein"
                     /protein_id="HNK44753.1"
                     /translation="MRKLSALLTALVLSSVASTALADIEVRRNGSSWARIEGDGTIRI
                     SGSSVGRIENDGTVRKNGSSVGRVENDGTIREHGSSVGRVENDGTIRKNGSSIGRIEG
                     DGDIRKHGSSWGRASNCCGDFGSKRTVAALLVFFSDYF"
     gene            complement(17156..18124)
                     /locus_tag="PKL17_08225"
     CDS             complement(17156..18124)
                     /locus_tag="PKL17_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44754.1"
                     /translation="MRVFGSSFKTILCGGLLSVFSLTLPAAADTAPATDAKSTDAKQA
                     DAKGSSGPVIEVIGPPPELTDYDSVPKALDAILQRAEKDAGQKPRVVAFGEYHQLKGK
                     THIPSALKRFTTELWASVQPLSSDLVLETFIPEGNCGKEEKKVVKDVATTTKRPEATE
                     SELVTLIKQAKAQGVQPHILQVSCKEYQSVLDEKGQVDYVKMLKLINDLLQKRISEVR
                     TRRKKASVDKVVMAYGGALHNDLYPMAELAEYTFGQALDHEFPGEYLEIDLYVPEYIA
                     ADKQVLSQPWYPAYKKMQKPGKVVVVRRGPASFVLLFAPMPAAPKK"
     gene            complement(18192..19700)
                     /locus_tag="PKL17_08230"
     CDS             complement(18192..19700)
                     /locus_tag="PKL17_08230"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNK44755.1"
                     /translation="MKRAILSVGLLLLGLGQLGCRRVALVEPIVPAEDLSLRGLDAPS
                     PKQQLQKAISERLRSREQMLREVYAPSSKWQALRIGQAAIDRRQLSIGRLVETGKELF
                     RVDFTVDEGLGNGLAAQKSTLAGDRPPPNLRHVQRGEFGGPDATRCAACHHLGGLGGG
                     GGKIDTVFLLGDGERPSSALPRNPKALLGAGLLQALGEEMTRELRVRLREADRQLAPG
                     QSVPLESKGVVFGPIKRRQDGKLDLTGIHGVSPDFIVRPFGWKGTTATLRQVVVEELQ
                     QHLGIQADELVHKQDPNRQAFLGDGPFDDPDADGVKDEATEGMVTALSTYIAALPPPM
                     EEVPEVATFSLRAARGFEVFEKIGCASCHVPTMKLSEPVISVGPSWEKRPRVDLSPLV
                     TDGRKAHEPIVVRLYSDLKRHNMGDALAEPYGYRGVPASDWLTPPLWGIASSGPYLHD
                     GRAPNVSSAIEAHGGEAAAAAKAYAALSTDDSAALRVFLQTLARPQVLEFKP"
     gene            complement(19697..21253)
                     /locus_tag="PKL17_08235"
     CDS             complement(19697..21253)
                     /locus_tag="PKL17_08235"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNK44756.1"
                     /translation="MILAALVLGSCHSGPPSAAGISETSTSQPLPSLGQQMSLSPELE
                     SYLADVLESQDVRRIGRYIAAVEPSEQRDDLNPSRHKASAKKELELTESLFQAGEELF
                     ETELAVVRGFGHAWGPQGPKMHRVHRGAKGGPDSFSCRSCHHRGGDDGAGEYNENALV
                     GGDGERVKSALERNPPSLQGGGLLQVLASEVSAALQSKVAGAPPGEPVKVDLKVEDVD
                     FGTVVRMPDGTLDTRGLRGIDPDLIVKPFGWKGTHSTLRRFAEEAFQVHHGMESGVLS
                     HYRRHFSTAPFFASDATRAIMAAFAGGDPHDLDGDGSRSELTEAMLTSIAVYLTLLPI
                     PTLEPPRAPDLLAAFRQGQAAFDRVGCSGCHKPVWTVQNPVWTERGEAQSGGFRIDMD
                     LRKDIRNGPSLINRDTEHVGFPVLLYSDLRRHDMGKELSDPAPAPGSPGPRIPPSHFL
                     TRPLWGLADSAPYLHDGRAQTISQAIVMHGGEAEESRRKYQALPVETQRAIQVFLLSL
                     TRPTLPEVSQ"
     gene            complement(21421..21495)
                     /locus_tag="PKL17_08240"
     tRNA            complement(21421..21495)
                     /locus_tag="PKL17_08240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21458..21460),aa:Pro,seq:ggg)
     gene            21747..22472
                     /locus_tag="PKL17_08245"
     CDS             21747..22472
                     /locus_tag="PKL17_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44757.1"
                     /translation="MTIESGILLGQTLVSTMPKSMPAHVKRAAVKLGKVADAAQKALA
                     RRQKELGQIPEETARDIDLAADQAWSALRDILDALSRLPSTYDRAKKARLLVSSVFPE
                     GTGFLRLPYSEQYVQMDTILRRIDESGLTKSIDAVVGPELLLEIRAVHLRYGAMCTRR
                     LNDKGPADSLTFHLRALQRSVVEYATSVASTVDSDDPASIALALDALRPIDNHRQQVA
                     SARRPESGPPATPDPAPSPPLAP"
     gene            complement(22748..23353)
                     /locus_tag="PKL17_08250"
     CDS             complement(22748..23353)
                     /locus_tag="PKL17_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44758.1"
                     /translation="MSRKPKVTILYEDARGQRNEFGLHRLVISAVRDDDAITAPYHEL
                     AAALEHRCQKGVDQVLRTCREDLPDIARDGRKVAVVVDEDVIRDKLRLKPCATLPQVA
                     QAIRALCAADMAPHIEVFVLSRNLESVLKAARDCVKEHRAEGPTDELFDRALCKDQLA
                     RDLVLSGVAKETHAKVRRCILHKISGLSDLVQWLGSVLSAS"
     gene            complement(23350..24588)
                     /locus_tag="PKL17_08255"
     CDS             complement(23350..24588)
                     /locus_tag="PKL17_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK44759.1"
                     /translation="MAITELRIAGLRTLADVRLSLRELTVLIGANGTGKSSIIEACEL
                     LRKAPSPDFLKEFRRTHWGFSGLLRHGADQLKLGVRIEEDGEVIDYDFALADREGKLT
                     FKETVGAGPNQRPRSTDWTYGEHPTHTELLVGFADRVQRSGNLEQMLANGLDDAARSQ
                     VRDRISVLGKVARALQSIEICLPFETLPWWASRSLKRPSAMREDNIVEPFERLEPLGS
                     NLGSAFFTLRNELGDAHWRETLELVRLGLGDHVEDVLVRAGGDGGKISLRVKYRSQLQ
                     PVPTFALSDGTLAFLAWVALYRAPRPQPAALLAFDEPELHLHPHLLMRVLDFLESLSQ
                     TSPVLIATHSDRLLDGLSRPADQAVLCELDEQGQTQLRRPDKEALDRWLARYRGIGDL
                     RSAGHDDSIWADAEPAADKA"
BASE COUNT         4647 a         7504 c         7372 g         5203 t
ORIGIN      
        1 agcgtcgccg tttcatcggc ccagtgcgca taggttggga attccggaaa cgtgcggaca
       61 aagatcgggt cggcgtagcg cttggccacc cagcttgcat agtgaatcat gcgaatgcca
      121 cgcagcgctt caatcatccg cagcgttcgc ctgtcgaagt cgcggatctg ctcgtaccct
      181 tcgatgatga tctcgcgctg ctgcgtcgct tctgcatcgc gaccgcgcac caccatccac
      241 agatcctgaa ccgcgggcgc catcatcaga tcatcgaagt ccagaaacac cggaccgctg
      301 ggctgccaca gcagatttcc gccgtgacag tcgccatgaa cgcggtgcag cgtcaatcct
      361 tgccacagcg gctccgccat ccgaaacagt gtgcgcgcgg cccgttcata gcgactctga
      421 agctgcatgt cgacaagtcc ggactgcaaa agactgtgca gcgcaggcag caaatattct
      481 gctggtgtaa gccgcggacg atccgctgca ggatgcgctg caccgacggt gtgcagccgc
      541 gcaagaagtc ttccgatctg cagaagctcc gcttcctgaa gttcttcttt tacccggcct
      601 ccgactttgg gaaacagcga aaagaagact ccgaaagatg tcttatgcag cgtttttcct
      661 tcggctccgg ccatcgcaat cggcgcgacc acgggaactt ccgcttcgga aagctcgcgc
      721 agaaaatcat gctcttgcac aagcgctgcg tgggaccatc gtcctggtcg atagaacttg
      781 gcgatgactt tcccaccgac gccagccagt ggttcctcta gctcgatttc atagacgcgg
      841 ttttccatgc tggcatggac aaaacagcgt ccggtggcgc gtatctttcc ggttggatcg
      901 agcgcttgct cgaccgctcc cagcactgca tccggcgtca atgaaaagaa atcgagctgt
      961 gagttcacgg gcagcgactt tgccaaagga acccagcggg cgggaattat tttcgataaa
     1021 gaccccggct ttcgacgcgc tttttgatcg cgtgcagcaa gttgggaatc gacttgcgcg
     1081 acatgtagtc ggcgacaaag cgcggaatcg cgcgacccgt atccacccag gtgcgataga
     1141 caagcagggt cttttcagtc gacacttcaa acagtgcata cccgccgacc gtgtctttga
     1201 tcgtgttgtc actggcgctc ttgtcgagct ggaagtcgat cgtgtgctgc gcttggctta
     1261 tcgaaaaaat ggctgtgtag cgcgccgtgc tcacagcggc atcgacggtc attttcgcgc
     1321 ggagtcgctc tttggtcgct tccaacactt cgactttttc cagccgcggc atgtattccg
     1381 ctttgtcggg aaagtgactg accacgctcc agacttcagc catgggtctg tcgatgacaa
     1441 tcgctccgac gccgcgtccc gatgccttgc cggtctctgt ggtgaaggac tccattcggg
     1501 tcggagtgtc accgcgcagc gccgcctcaa gttctggttc cgtaagtcct accgcagcag
     1561 ccacaagcag accaaatagg tttgccgtgg cccagatgct catcatgaat cgacgctatc
     1621 acgctttgcc ccaaacccga cagacccccg tcacgattcc aacttttggc gcgaccgcaa
     1681 aaaatccaga ctaagcttag gggtgagaaa ccatcatgcg taggctgctc cgcatccttg
     1741 tcatcagtgc gtgcgcggga acggtctttt cgccgcagtc cggacatggc tacgtgcgga
     1801 cacggaccaa agtctcctgt cggccactgc gctgggctca gacttgtatc tttcttcaac
     1861 cggatgcgga gctgcttcgc gatgacttgc ctgccgatgt cacgttgcag gcgattcaaa
     1921 gtgcaatcgc cgcttggaat gatcggctcg caccatcgag ttttttgcag ctccactatc
     1981 tgccgccgac cgattcacgc gaagcgagcc agaccgatcg cctgggatgg atcaagttcc
     2041 gctttggttc atggtgtagg ccgccaaaag acagcagcag cagtcctgtc tgttttgatc
     2101 cttcggccac cgcagtgacg accgtttcgt ttttgaacaa accgacagag cctgatctgg
     2161 atggccaaat cgtcgataca gacatcgatc taaacgccgt tgggtatcgt tttgtcgacg
     2221 ggtcgaagcc tctctcaact ggggatcggc gctctcctgt cgatctgtgg aatgtgctta
     2281 cccatgaact gggacatgtg atgggacttg accatacttg tctgattgat gggggatcga
     2341 gtggttgcat gaccgatcac aaaggtgcgc aagttgtcac ctgtgcaacg ctcgatggac
     2401 agcgcttttc ttcgcaagat gcagagactg cttatgaagc cgcgatgtat ccaaccacga
     2461 cagcggggga ctactcgcga cggattccta cggcagatga tctggctggc gtgatcacga
     2521 cacaccctgc ttcggctgat ccccaagtct gttcgcttcc gtcggtagcg agcggctgca
     2581 cggtgtctgc acaggtccag tttgggaatc cccctttttg ggcagcgctc ggtggactgt
     2641 cggttttgct ggcgctgtct ttgcggctgc gtcgtcgctg attgctgatg ttcgttctgc
     2701 gccaaactcg tgatacgcta cagccttccg agcacatggc tcacccaatc accgaagggg
     2761 gaacacgcga tgatcaagct gtatggaatg acgctctccg ctcacacgcg caaggttcag
     2821 tcggcactta tcgaactggg tctgccgtat gaaatgatta ccgtcgattt gaccaaaggc
     2881 gagcagcaca agccggaata tctggcgctc aatcccggca acaagacgcc gacgctggtt
     2941 gatggtgaca cggtggtgat cgagtccaat gcgatcctgc tttaccttgc cgaaacgtat
     3001 ggcaagggca agctgattgc agagagcggc aaagatcgtt ggcagaccgt gcagtggctg
     3061 gtctggaccg catcggaagg tcatggtccg ctgtcgcgtc cgtggtatgt gaaagttctg
     3121 tttccgatga tgggtcagcc gctggacgaa gcgggtcttg ccaaatcgca tgaagatgcc
     3181 agggttccgc tgaaggtcat ggacagcgtg ctggccggtc agccatactt tggtggaagc
     3241 aacttctccg tcgctgacat cgcacttgca gagtcggttc acctgtcgca gtggggcggg
     3301 cttgacacca gctcctttcc gaacgtgacg cgctggttcc atgatgtgac ctcgcgcgat
     3361 gcctacaagc agtcccgttt ccagcacagc gccagctagt tttgcggcgt cttctggaat
     3421 ccgcttcctt cacgatcatc gtctgaaaaa tcccccctct gtctccgttt cggctgccgc
     3481 tttgcggagt ggaatttgtc tgcccgctgg caacttcctt gcgtctttgg gattccgaac
     3541 atagtctctt ttgggactcc tatttctgtc ttgaggtgac gtatgaaacg gaatcacggt
     3601 gcttgtgttg gtcttttggc ttgtgcgctg ggacttttgg gtgtgggaac cgcaaacgca
     3661 gaagaaattg gcagcgtgtc gacggtgttt aagatggtgg ggccgaacga caagattgtg
     3721 atcgaagcat ttgatgaccc caaagtcgat ggcgtcacct gctatctgtc gcgcgccaaa
     3781 aaaggtggaa tcaagggcgg actcggcgtg gcgaccgata cctcggatgc atcgatcgct
     3841 tgtcgacagg tcggcccgat caagatcaag gaaccctttc ccgatggaga ggaagtcttc
     3901 aaaaaagaca cctcgttgct gtttaagacc atgcaggtgg tgcggtttct cgacagaaaa
     3961 cgcgatgtcc tgatctatct ggtctattcc gacaagctga tcgatggctc gccgaaaaac
     4021 tctgtcaccg cggttcctct gcgaaatccg acaccatagc gcttacttca tccaggcttt
     4081 cactgcgatt ccgaacggat cggtggggcg acttgggtcg ggaggattcg acggcagaac
     4141 cacgccgctg ccatttgtgc caagcacaag atgcggcata tcgccaaccg accacgcaag
     4201 tgcagacagt ccttttccgt cgatgtaacg gtgcagtcgt gccatccagt cgcgcgccgc
     4261 ttgcgcatcc atgatcatct catcgacgcc gtattccccc aggatgacgg gcacatcgcg
     4321 cgatacattc ccaaagtagc gatcccagtc cggctcgacg tgcggcggcg ttcctttgaa
     4381 cggatacgga tgcgttgcat agatgatcgg tccgagtccg ctgctgatgc gattgttcgc
     4441 gtcggcaagc gccgatagat cgtaggcgta atcgagtcct cccaccacca ccacggtgtc
     4501 ttgtgccccc gcgctccgaa tcgttttgat aagcgactct gcttgcgttt tccagacatc
     4561 ccacgacgag gcacgcggtt cattccacag atcgaacagc acatgggatt ccttgccaaa
     4621 gcggcgcgcc agctgatccc atagaacgct tgtctgccca tccggaagcg gcggattcag
     4681 gttgtaggaa tagccgtgga cttccaggat caggtactgt ccttcgcttt gtgtggcgct
     4741 gacaatgttg ccgatgtcat cgcgatagct cgcgcttccc atgtagtggt cttgcgcaat
     4801 gggaagtcga acagcgacgg cattccattc acttcggaag cgatgcagct cgtcttgcgg
     4861 attggacgga aagccggcgt ttcccagcga tgacttgtgc tgaagacccg atcgattgat
     4921 tccttttaat cgcaccggaa cttcgctgcc atgacagacc acgccgagct gattcccgcg
     4981 cacaatgact cgcggtagct gcgtacttcc gcacgtcgca ggatcggaaa gttggtttgg
     5041 cggcggctgg tctgggctct gctggggatc gcttccgcag ccggcagaca gaaacaaact
     5101 ggatgaaacc aaggttgcga tcaggaaacg gatcatcatt ccgaaagttt acttggtttc
     5161 cttttcttca gtcttctcgt cgctgctggc ttcactttca gcgctctcgc tttgcagctt
     5221 cactttgatc tcaagccggt ctttgtcttt ccatttcacc cgcttgcgac cctttttgta
     5281 tccttcaagc ttcagctcga tcttgaagtc ttctcccggc gtgcgaccga gcagaaggag
     5341 cggcgtcacg cccacttcct ctttgtcgat tcgcaccgat gctcccggtg gttcgctgcg
     5401 taccaagact tgtccggcgc tcggcttctg tttgtcttcc gctggcactg cggaaggttt
     5461 gcttttgtcg gttgtggcga cgggagaacc ggcttctccg tttggctttg tggtttcgct
     5521 tcccgcatca cttgctggtg gtttgtccgc aagatccgaa agcggtgcaa ggtcttggac
     5581 cgccagatca tccggggaca gactgcgctg gctccgctgc ggaccaatga ccaaaaacca
     5641 tgtcagggct gcggacaaaa gcgcagcaga gacgccggca agcggaaaca gccagcgatt
     5701 gggtgatgga cccacttcga cggacgcttc tggatcaggt tggggggcgc ggagatccgc
     5761 aggtgcactc agcgcttcgt gcgtaagctc ggtcgatatg atgtcgatct gtcccgatgg
     5821 ctcggcgaca atcgatacag cgttgcgttc accgctgctc tgtccggcct ttggtggagg
     5881 ttttttctgt tcttgtttgg ccagactgcc gagcggagca aagtcatcgc tctgcgccaa
     5941 ttcaacggca ggtcgtcctg gtgcgctgcc gccgtcatcg tatgacacat cgctctgcaa
     6001 aagttctagc ttggcttccg actgacgcgg gcgttgggtt tctctgatcg gttcgccaga
     6061 agactgcgac gatgggcgac gggttcgctt cgatcgctcg acccctggcg gcagcacaga
     6121 gacaggtgcg accgcggtga cgctatcgcc cagaccatcg gcctgattct tcggataaag
     6181 cgatccactg ccgtcgcgtc ggatcgctgc gccgacctgg ccaagctcgg tcgggcgtgg
     6241 caaaaggtcg gctccttcgg tcaaaagtcc aaagacttca ccatcggcag taatcgctgg
     6301 aaagaccgct ttggggggcg ccgcttcaac cagtcgtccg gaaatccgcc tgtgggttgt
     6361 cacatcggag tgatcactgg gccgcagtcc gcgatcggtg cccgtgcgcg cgtcgtcggc
     6421 gtcgtcttca tcgtttctgc ccggcgcttt ccgttcgagc agggctttca gattgcccga
     6481 ctgaaaaagc agactgttgt cgtcttttat ccccagcgcc cgcatctgct caagctgcgc
     6541 cgcatcaatg cccatcgtcg gacttggcga gcgatccgcg tcctcttcat ccacatcggg
     6601 cgcggacatg ctctgcttgg ctgccagtga cagatcgagc gcggcagtcg gttcatcggc
     6661 accgcgtttt ttgacctgcg tggtctgcac tttttcgacg aacttgccaa gctcgctcgc
     6721 ttgaaatgtc ggtgcgtgcc tgcgcaaaaa accgtcgagc gcctgggcca ggtcttccgc
     6781 cgttttgtag cgatcatcgg gcttgcgacg cagcgcccgc atcatgattc gctctagctc
     6841 ttgcggcgta tccggacggc gccgcacgat ctgcggcacc tctgctttgc gtactttgtc
     6901 gagcagccgc tcgatgtttt ggtcaaagta cagcatctcg cccgccagca tttcgtacag
     6961 caaaatgccg catgcaaaga tgtcagtccg tcggtcaagc ggctctcccg ccgcctgttc
     7021 cggactcatg tagtagtact tgcccttgat gatcccaagc tgcgtctgtt ccgatcggcc
     7081 ttctgctttc gcgatgccaa agtcggtcag cttgaccgcg ccttcatagg acagaagcac
     7141 attctgcggt gacacgtcgc ggtgcacgac atggagcggt tttcccgccg ggtcggcaag
     7201 ctcgtgggca aagtgcagac cgcgcgcaag ctcgcgcgca atgtaggcgc agagcggaac
     7261 cggaatgctc agccgcagat cggtcgcgcg ctgttcgagt cggaacaggt cgacgccttc
     7321 gaccagctcc atcaccaagt agtactgttc tttgtccttt cccagatcgt aagtggtgac
     7381 gatgttggga tgtttaagcc gcaccgcgag tcgcgcttcg tcgagcagca tgcggacgaa
     7441 gtcgggatct tcgctgttgt tcgggtgaat gactttgatg gcaacttgcc gtagcggctc
     7501 ggtgtcggga acgatggcgc gatagatttc ggccattccc ccgacggcga tcttcttgac
     7561 gagctgatat ggaccaagct gccccgtcag cacatgcgtc cctcgaagtt gacggctcgc
     7621 ggaaaagtga gcggctgccg attagacatt gtcaatcatc ggagagcgcg tgcctagcca
     7681 gccgcgtggc ggctattcct tgctcgtcgg attgccaggg tggacaaaca tcgacagcgc
     7741 gcgcagcggc tgccgcgttc ctctgtcaaa agtacagacg acttcggtgg ccatgccggg
     7801 ggcgacattc accacatatt gcgtcgcggc gttcacaatc aaatagagcc cgagcccggc
     7861 accatacgtc ttccgatcga tctggtccga gcttttgagg cacttttcga tgtagcgcag
     7921 gatcgtcgct ttttcgagcc gaccaaacct atcgcgcacc gagacagcaa actgatcatc
     7981 ggccactgca tagcgaatcg agacgggacg cggcgacagc ttgtcgactc gctctttgac
     8041 ttcgacgtga tgaaacagcg gattgccgtt tgcgtcgacc ggcgcgtcat agagcgcatt
     8101 catcagcagt tcttcgcaga cctgcccgat cgacgcgcga acctgtcgac gcacgcccga
     8161 tttgtcggca aacgacagca cttcgtcgat cgcgttctgt ctgccctgaa aatcacgcag
     8221 ccgcgtcagc tgaatcggcg ttcccggcgg caggtagcgt tccagaccaa acagctcgcc
     8281 ggaaacgaac ttgccgatcg tcacttgcaa aaggaacatg ccgcgctcgt cggcggtcag
     8341 tacgtgattg cagtgcgaat cggaaagcag cgcgacttgc tcggcgagac tgcgctgcgg
     8401 aaccattgca atcagctgtg ccgagtcgcg aagctttcga cacaacgtct tcatccagtc
     8461 gagcttgccg tcccacgccg cgatcaccag acgatgcggc gcttgcggac cggcaatttc
     8521 gtggacacca gcgacggcaa cgggctgaac tcctgccgtg cggacgactt gggatagtcg
     8581 ctgtcgcagc accggatctt cggtgacgat caggattcgc tgtccggacg cagctggttg
     8641 ctggaaacta tccaccgttt gcgagcgtcg cagcttgcga ctgtcgctaa gcgtataatt
     8701 tccagaggaa aagtcaacgg ccttcgtcgg ggtggctacg cagagctaac gaaagaggcg
     8761 gacgacggtg ggcgtgtggg ggcgcgtcgt ttgggcggga acgggaagct gcgtcttgtg
     8821 ggtgcggtgc tgcccagcgc ggtcaaagta gaaaagctcg acggtgaatg cctggtcata
     8881 agcgatgcgg tagatggttt cacgctcgct cgattcacag tcgacggaaa tgggaccaaa
     8941 cagcgttggg agcttatgcg ctgcaaagtg ctgaccgggc gcaaaatagc tcttgggcaa
     9001 agccgcgccg acgagcaccg ttcccgttgg caaatcgtct tcgtcgcgtt cctcgctgac
     9061 cacgagaaag cgcagatact gaagcatcac cgccgtgccc gccgaacagg ggtcggaatc
     9121 gaaccggcaa ggctgctggg atagcgcttt cattcggtcg cgccactcgg tgtcgtcaaa
     9181 cagcacgatc gcggtttccg gcgaaacgaa cgtgtacgga tcacagctga gcgcgacttg
     9241 tccgagcaag cccaggtaga accgggaaaa atcccgacgg tgcagcgcgc agagctgggt
     9301 gccgcgcgaa tactcggcat cgacgcccag acgaccaaac cagtcgtcaa agcgagccac
     9361 ttgcagcacc tgtcgtccgg tctgctccag gtagtcggtg atctcgtggg aaaagcgacc
     9421 atgccagcca aacagcgcgg tttccagaat cagcgacgca aacagctgat gatagtcgcc
     9481 tgcgctcggt gtctttccgg tttcgtcggt gcgaaacggc agatacggcg gcgtgccgtt
     9541 gtgctgaaag gaccgctctg ccgccaacag caggttgcgt cgcgtttctg ccgcttgcgc
     9601 aagccatgaa tcggcgcgcc catcgccggt gattttcgcg agtcgacccg catcgcgcag
     9661 cccgcgccag cacgccgagc tgccatacaa accaaacgcg ggcgtcgtca aatcaccgcc
     9721 ataggtatgt ttgggcaaaa gtccgaaatg aatcggtcta tttccgtcga caagctgctg
     9781 cgtgctgcga aacgacgcgg cgatccactc ggcactttcg gcgatctgcg gccacagcct
     9841 tgccagcagc gtgcgatctt gggacagcgc atacacgtcg gcggcatacg tcagcgccag
     9901 accgttgcga tactgatggt gctgaccctc tttggcgaga aactgcggat cgaaaaacgt
     9961 ccgctccagc acgcgcgccg cttcgtcgtg gtgtccgtac tgcgaaagcg cgacgatgtt
    10021 ccagccttcc tcgacgccaa acaccccacc atcgtagacg ccagggaaca gtccgtaccg
    10081 catcacgccg ccctgcacga acaggcgatt gtgcaaaaga agcgcacgcc acagcttggc
    10141 aaacagcgga tcgggaaacg acagctcggc gccacgctgc agatacgctt cgcaatcggc
    10201 gctgagaaac tgctcggctt cctcgaaact gcgcagcggc gagtgttcgg ccagcggaat
    10261 cgtcacgtcg agccggactc ccccgtcgac gcgctcaagc gatcgcgttc ccgttcgctg
    10321 cgaaagcaaa atcgtcagtt cctgggcgtc gccaggctgt ctggaaatgc gctgtgccag
    10381 cgcttttggg tcgttttcgc agcctgaaag accgcactgc cagacttcgt cttcgatccg
    10441 ttctttcgtc agtccccgca gggtgtagcg tgcgcgcaga cagtgctcgt ggtcgacaaa
    10501 gacccgctgc gtcagcgttt tgtcgccggc agaaagctcc acttccgcga tcggcagata
    10561 gccgcgctgg agccgctgct ggccgtgcat ttcgttggtt tgtagccagt cggtcgccaa
    10621 ccgatagagc gcccgatcgg tgccttcgcc ccactgccgc agcacgattc cgccgtccca
    10681 tagctgatac gcgatcggtc cttcgacttg cccaagcagc gccccaccgc gaaacggcag
    10741 atgcagctgg tgcagatcag ccaccgtcgg atcgcttgtt cgcttttgca gcgtctcggt
    10801 gagctgatcc ccgtgcttgg ggagctgtga actggacttg ggctggctcg gcgcggctgg
    10861 aagtggaagc gctggtgcgg cctgcagcgc cagtttttcc gtcgagacaa tcacccgcgc
    10921 aagctgatgt ggacccgccg gcagccagat cacaagctcg ctttcggggg ccatcggttc
    10981 gtcgggaaaa aacgccagga tcgtcgctgg aaaaacggcc cgcgtctggg gcggaatcag
    11041 cgttagcggc agcggcagcg tttcccgccg gcaagggcag ggcgatccat ttgcatccag
    11101 ccgaaggtcg ttttccctgg gcggtcgtgg tgatcgccgc cagctattcc aggtgtcgac
    11161 catgccttcg ccggcaaaca gcagatacag cgcgacgatc ggtgcttctg ccccgacgcg
    11221 cagtcgcagc cgctgcggtt ttgtgatcgc gaaggtcttt tgcgcaacca gatcgtcccc
    11281 gacgagaaaa tcgggacagt cacttgcttg atctttgtcg tcgagaaaat cggcttgcag
    11341 gcggacgcga cccatttcct catcatcgcg gaattggggc aggctgtgct tttttcccta
    11401 gaagcggagc gtcaggcttg gcacaagctg cgtcggctgc ggcgcgccga tcggctgaac
    11461 aaaacgcgga ttttcgatcg gctgggtcag gctatacggc tgaatgcgca gcggcgctgc
    11521 ctggatcgtc gtgcagacat cgacatcttt gcagatcgtc tgctgcgaca gcacaaccgg
    11581 aacgatgatg gcggttgcga ccacaaccac accgacggca acgatcgtcc agacccacca
    11641 cttttgatac cactttacct gcggctgctt caccagatac gggttctggt cggcgggcag
    11701 tggctttagc tcgacgtctt tgtcgatgcg cttgccgggc tcggctttga tggcaaaggt
    11761 ctccgagtag tagcccggac gctcgacgcg aacttggtga ttgccgagcg gaacttcggt
    11821 ttccagcggc gctttgccaa agaacttgtc gtcgatgaaa acccggactg ccgggccgtc
    11881 tttgaccgat gtcgaggtaa tgccaagcac gccggaaatc ggggtcagat cgacttcgat
    11941 tttgctcgtc tggccgtttt tggccttgat gacgtcgatg taaggcgtaa agccaagccg
    12001 agagacgcgg atggtgtgct cgccgggcga aagcggcacg atgtccttaa aaggcgtttg
    12061 gccaatcagt tcgccgtcga tggaaagctc tgcgccttcc gtcgcgcaga tcactttgat
    12121 ggccgtcttc ggggcacttg gtgctgccgc tgctgctgcc ggcgtcgcgg gcgcagcgct
    12181 tggggccggg gcagcgcttg gggccacagg tggcgccggg gccacggcag gcgtagcgct
    12241 tttcggcgga cggcgcgccg cagcaatatc aattgttccc gaagaaccaa ccaccaggag
    12301 cagcagggac aaaaggccga tccagacgcg catcgaggtc atcttatcaa acttcctagt
    12361 cccacgtcgg cctgtggtcg ggaaaatttg cgacttggtc tgcagcgtcg gggctagcgg
    12421 cggatgttga cgaaatcata ttggaggttg tagctgtccg cgtcgctgat ggccccgaga
    12481 cgaatcaatt tcaccgcgtt tcgcacgcag gccgtttggt cgccggcacc ttcgacgctg
    12541 gcttcggtga ttttgccgtt tttggcgacg gtaagctcga cgctgatttt ggtaggtggt
    12601 acgacttcag cgggatagca gcgaccgact tgcggtgtgg ctttgcgcaa gatggcattg
    12661 gcggccatgg ctgcttcctg ggtggtcacc ggttcgtttt tcgcatcggc caccggagtc
    12721 gctttgcgtg ccggtttttc ggctttggcc ggctctccgt ctttgtcgtc tttggccgtg
    12781 ccgtcgcggc tcgcggtgtc tttgctcgtg gcctctttga ccaccgcttc tttgctggct
    12841 gacgttgccg caccgtcttt tgcgggcgct gcggtttttt cggcagccgt ggccgcgtct
    12901 ttgctcgtcg cttctttggc ggcgggtttt tcggcagcga caggattgcc cgcggcgctt
    12961 gcggactctt tgccatcctt ggcatcgggc gctgggccgg caaatggcac cgccgaggca
    13021 ggcgccggca gtcccagcat cgccaggttg gcgtggcgcg gtggtccaaa acgcagtgga
    13081 tgcgtcggtg gctcgccgat ggctttcgcc cgcggtccac gcagcggcag gttgggcgga
    13141 gtgtgtacgc gcgggctcgt cgctgcggcg atgccagttt gaccctgtgc tccagcgtcc
    13201 ttggcttcgt ctttttcatc ccccgtcggc aaaaccggcg acggactgtc tttgggcagc
    13261 gctgccaccc cgagatccgg cgtcactgcc ggtggtgcag ccttggcatc cggactgctc
    13321 gcaacacctg tcgggtccgc tggtttgggg tctgtgacgc cgcttacttt gatgagcgga
    13381 tcttggggaa ccgtcgtcgc cgcattgggg tttggaatcg gtgtcttttg ctgactctgc
    13441 cacagcgtat aaccggccaa actggtaaat ccaagagccg cagacaccgc gacagccgca
    13501 atggcgacgt gcgatttccg cagaccgggc ttcggcgccc caccttgcac cagctcgcca
    13561 ttttttatca ccgacgcagt cgagctgtcg ggcagcatcg acttgaccac cgccgggacg
    13621 atgacaattt ccggtccgga ctgcgactca cctgccgatg gtgctgcgac aagcgcttcg
    13681 tgctgttgcg atcccgttgg attcacaccc gacggtggta gtcccagaac cagcacgccg
    13741 ccttgcagct tggttcccgg tggcagcgtg tcgatccgtg gctgcggctt gggatacacc
    13801 gcgttcagcg agtgcagcag cgacgtcgca gactgatagc gatgttcggg tttttgctga
    13861 cagcagcggg tgatgatctt ttcaatcatc gtcggcagac cgcgttcggt aaacgggagc
    13921 ggcggcagct tggctgccat gatcatctgc cgcttttgct cgaagtcatc acacgaaaac
    13981 agcttttccg ccgtcagaca ttcatacaaa agcacaccga cggaccagat gtccgatcgg
    14041 gcatcgacca gatgcgagtc accgcgcgct tgctcgggcg acatatacga cggcgatcca
    14101 accaaccgac ccgatccggt gatttccacc ccgtcgagca tcttcgacag accgaaatcg
    14161 agcactttca gcgtcagcgc cggctctccc ggtggcggat tttcgacatt cgcgagcaga
    14221 agattttccg gtttcagatc tcggtggatc acgccgcggc ggtgggcgac gaccagtgcg
    14281 tcggccagct ctgccatcag ccgcaccgat agctgcggat cgagcggtcc tttttcgagt
    14341 cgatcgcgca gcgactgacc ctggaccagc tccatcacca gatatggcca gtttgtcccg
    14401 tcgtcgcgga aatcggtgat ggcgacgatg tgcggatgtc cgagctggaa cgcgctctgc
    14461 gcttctcgac gaaagcgagt gcgcagctct gggtttgaca caagctgcgg gtgcagaaat
    14521 ttgatcgcac aaggccgctg caggtgcagg tgccaagcgc gatacacgac gccgacgcca
    14581 cctttcccca ggggcgcatc gagtcgatag atgctgtcga gcacatcgcc gcccttaggg
    14641 gctcgacgga atgagctgtg cgcgggctct aggtcttctc cgctctcagg ctggtcgctc
    14701 atcttgggct acgccatgcg ggcaaagcgc acggaaatac aaagaatcgt aacacgaact
    14761 actcatttgc agagtggcga tatggtgctt tgctccactt tggtggtttt tgcgggccag
    14821 attgattttg tccgcggcct gctgcgctac cctgtgcgca tgtcgactgg ttcaaatcgc
    14881 gacggtgatg gcgatcgcat cccgccccac gagctgccgt ttccggccag cttgtgccac
    14941 ctttgccaag cgccccgcta cattcgctct ggcaaaggca gcgtctttgt cctgtgtccg
    15001 ctgcttcccg aaaagtatcc gcggcagccg gtgcgcactt gcgcgctgtt ttcgccgcgt
    15061 gccgacgacg gttcgagttc gtcagaccgc tgacaagtca aattttttcg cgccacgcgc
    15121 cgcccaagac acggtatata gccagcacgc acttgtagtc gtctgtgcgt ttaccgtctc
    15181 aatcccggct ttttcggagg atccctacga tgcgtggtga caagtcccgt atgcagagcc
    15241 agcgcctgct tcttgctgcg tccgcggcga gcttgctctt tttggcgcag agcgcggccc
    15301 atgcggaagt acatctggcg ataggttcgc gcttcgagcc gtatcgctac acgatggcgc
    15361 agtttcccaa tgcggcgacg gcggctggcg accttgcggt ggccaatcag agcttccaga
    15421 ccacgtcgct gtcgccctat tttggcgcct tcttttcgca gcgctacggg cttgtgctcg
    15481 ggctcgatct ggggtgggcg aagcaaaata ccgaaaagca gatgggggca aatcctgcgc
    15541 taaccgcgac ggacagctat ctccagttcg gtctgggcct gggcttcaaa atgtacctgc
    15601 gcgagccgcg caaggatcgc attgcccctt acgcctacgt cgatgtgttc aagtattttg
    15661 cgtcggtgtc gacgaccaac acggcggtca ctggcgatca ggcgtcagcg caagccgata
    15721 cgtcgagccc ggtgggcggc acgctggcga tcggcgctga gtactttttg gggaccggct
    15781 tttcaatcgg tgctgaagtg ttcggtctgc gcgtctcgca cgtcggggct gaatacaccg
    15841 atgcgacgat gactcggcgc agcgttggct ttaccgaact gtcgatgtac agcggtctga
    15901 cgctcaacta tcgctttgcg gtgggttcgg gatcgaagtc agacgagcca gaggaagacc
    15961 ggcgacggac gagcacgccg agcgcgccgc cgccaccgag cgtgccgccg ccgacgcctg
    16021 aagcgatcga ctagcgcacg cagtggtttc gacggttacg cagctttcgg cgacgggttt
    16081 ttcgtcgctg ggctgcgcag tcgttccaaa agccagtccg cgaacacttg cgcttcgacg
    16141 tgtttgccaa ttccgatggc gcggttgcga ccgatgagcg ccaccacctc gcactgatgc
    16201 attccccgac ggttgcgctc tgccgaaaat cgttgccata gcttctcaat cttggccacc
    16261 gaatcccgcg ctgacgctgc gtcggtaaat ttcagcaccc cttccagctc tttggcgtcc
    16321 tcgttctgcg gtcccgtcga ggtatagagg tcgacatacg ccggaacccg tccctgatcg
    16381 atgagtgcgt tggcgacgta gctgggttcg cagcgcagca gtttcgcgat cgccacgacg
    16441 ttcatgcctc ggtcgtacat cgaaacggaa agctgctttt tggtcagtcg gtccttcatg
    16501 atcgctcctt tgccttgggg tccaggaagg cacatggggt tttcccccca aaagaatcgt
    16561 cgcacagggg tctgacagcg tcgggcggcc aaagcgatga gtagaaacgc ccaaagaccg
    16621 tctatgtttg ccacgccgat cggcacttgg gctgtgcggt tccgatctgt gaaaccgtcg
    16681 cccatgccgc gaaaccagac cgaacacgtt cactgccaaa ggagaaacca tgcgaaagct
    16741 ctctgcactt ttgaccgcgc ttgtcctttc ttccgtcgcc agcaccgcac ttgccgacat
    16801 cgaagtccgt cgcaacggtt caagctgggc ccgcatcgaa ggcgacggca ccatccgcat
    16861 cagcggatcg agcgtgggcc gcattgaaaa cgatggcact gtgcgcaaaa acggcagcag
    16921 cgtgggccgg gtcgaaaacg acggcaccat ccgtgaacac ggcagcagcg tcgggcgggt
    16981 cgaaaacgac ggcacaatcc gcaaaaacgg cagcagcatt ggccgcatcg agggcgacgg
    17041 cgacatccgc aagcacggtt cgagctgggg acgggcctcg aactgctgcg gcgactttgg
    17101 cagcaagcgc accgtcgcgg cgctgctggt gttctttagc gattacttct agccgctact
    17161 tttttggggc ggcgggcatc ggggcaaaca gaagaacaaa cgatgccggc ccgcgtctca
    17221 cgacgacgac tttgcccggc ttctgcattt ttttgtacgc cggataccac ggctgcgaaa
    17281 ggacctgctt gtcggcggcg atgtattccg gcacgtaaag atcaatttcc agatactcgc
    17341 cgggaaactc gtggtcgagc gcttgtccga acgtgtattc ggcaagctcg gccattggat
    17401 acagatcgtt gtgcagcgca ccgccatacg ccatcaccac tttgtcgacg ctggcctttt
    17461 tccgtcgggt gcgcacttcg ctgattcgct tttgcaacag gtcgtttatc agcttgagca
    17521 tcttgacgta gtcgacctgg cctttttcgt cgagcaccga ctgatactct ttgcagctta
    17581 cttgcaagat gtgcggctgc acgccttgtg ctttggcctg cttgatcagt gtgaccagct
    17641 cgctttcggt cgcttcgggc cgcttggtcg tcgtcgcgac atcttttacg accttttttt
    17701 cttctttgcc gcagttcccc tcggggataa acgtctccag caccaggtcc gacgacaagg
    17761 gctgtaccga tgcccacagc tccgtcgtga aacgcttgag tgcgctcggg atgtgggtct
    17821 tgcctttcaa ctggtggtat tcgccaaatg cgacgacacg cggtttttgg ccggcgtcct
    17881 tttcggcacg ctgcaagatc gcatcgagcg ctttgggaac actgtcgtag tcggtgagct
    17941 ctggcggtgg gccgatgact tcgataacgg ggccagaaga tccctttgca tcggcctgtt
    18001 ttgcgtccgt tgattttgcg tcggtggctg gggctgtatc ggcagccgcc ggcagcgtca
    18061 gcgaaaaaac agacagcagc ccgccgcaca ggattgtttt gaacgaggaa ccaaacacgc
    18121 gcatcgtcat catcctaccg attgccggtt tggagtgccg ggcgagcgct cggttccgcg
    18181 caaaaaaaac actacggctt aaactccagc acctgcggac gggcgagtgt ctgaagaaac
    18241 acccgcagtg ctgccgaatc gtcggtcgaa agcgccgcat aggctttggc tgctgcggcg
    18301 gcttctcccc catgggcttc gatggcgctg ctcacgttgg gggcgcggcc atcgtgcaga
    18361 tacggtcccg acgaagcaat cccccacaaa ggcggcgtca gccagtctga tgccggcacc
    18421 ccgcggtagc catacggctc ggcgagcgca tcgcccatgt tgtggcgctt taggtctgaa
    18481 tacagccgca ccacaatcgg ctcgtgcgct ttgcgtccat cggtgacaag cggtgacaga
    18541 tcgacgcgcg gtcgtttttc ccacgacggc ccgaccgaaa tcaccggctc gcttagcttc
    18601 atcgtcggaa cgtgacagct agcgcagccg attttttcaa acacctcgaa accacgcgcc
    18661 gctcgcagtg aaaaggtcgc gacttcgggc acttcttcca tcggtggggg cagcgctgcg
    18721 atgtacgtcg aaagcgccgt caccattcct tcggtcgctt cgtcttttac cccgtcggca
    18781 tcgggatcat caaacggacc gtcgcccaaa aacgcctgcc gattgggatc ttgtttgtgc
    18841 acaagctcgt cggcctggat gcccagatgc tgctgaagtt cttcgaccac cacttggcgc
    18901 agcgtcgctg tggtgccttt ccagccgaat gggcgaacga tgaagtcggg gcttaccccg
    18961 tggatgccgg tcaagtcgag cttgccgtct tggcgtcgct tgatcgggcc aaacacgacc
    19021 cctttgcttt cgagcgggac gctttgaccg ggggcaagct ggcggtcggc ttcccgcagt
    19081 cggacgcgca gctcgcgggt catttcttcg ccgagcgctt gcaaaagtcc cgccccaagc
    19141 agtgctttgg ggttgcgagg cagcgccgag cttggccgct ccccatcgcc gagcaaaaac
    19201 accgtgtcga ttttgccgcc accgccaccg agcccgccca agtgatgaca ggccgcacag
    19261 cgagtggcgt cgggtccacc aaattccccg cgctgcacat gccgcaggtt cggcggtgga
    19321 cggtcccccg cgagcgtgct tttttgggcc gcgagcccat ttcctaggcc ctcatcgacg
    19381 gtgaagtcga cgcggaacag ctctttgccg gtctcgacca gccgtccgat cgaaagctgt
    19441 cgcctgtcaa tcgccgcttg cccaatccgc agcgcctgcc acttgctcga tggggcgtag
    19501 acttcgcgga gcatttgctc gcggctgcgt agccgttccg aaatcgcttt ttgcagctgc
    19561 tgctttggtg acggtgcatc caggccgcgc aggctcaggt cttcggctgg gacaatcggc
    19621 tcaaccagcg ccacgcgacg acagccaagc tggccaagcc ccaaaagcaa aagtccgaca
    19681 gagagaatcg cccgtttcat tgcgacacct cgggcagcgt cggacgtgtc aaagacagca
    19741 aaaacacctg aatcgcgcgc tgcgtttcga ccggcagcgc ttggtatttg cgtcgggact
    19801 cctcggcttc cccgccgtgc atcacaatcg cctggcttat cgtctgggcg cgaccatcgt
    19861 gcaggtacgg cgccgagtcg gcgagccccc acagcggtcg ggtcaaaaag tgcgacggcg
    19921 gaatccgcgg tccgggtgat cctggggccg gcgctggatc ggacagctct ttgcccatgt
    19981 catgccgacg caaatcggaa taaagcagca ccggaaaacc gacgtgttcg gtgtcgcgat
    20041 tgatcagcga gggaccgttg cggatgtctt tgcggagatc catgtcgatg cgaaaaccgc
    20101 cgctctgggc ttcgccacgt tcggtccaga cgggattttg gacagtccag accggcttgt
    20161 ggcagccgct gcagccaacg cggtcaaacg cggcctgacc ctgacggaat gccgccagaa
    20221 ggtcgggcgc ccgcggcggt tccagcgtcg gaatcggcag cagcgtcaga tacaccgcaa
    20281 tcgacgtcag catcgcttcg gtcagctcac tgcgcgagcc atcgccatcg aggtcgtgcg
    20341 gatcgccgcc ggcaaacgcc gccatgatgg cgcgggtggc atccgatgca aaaaacggtg
    20401 cggtcgaaaa atgccgtcgg tagtggctca gcacgcccga ttccatgccg tggtggactt
    20461 ggaacgcttc ttcggcaaac cggcgcagcg tgctgtgggt gcctttccac ccgaacggct
    20521 tgacgataag gtccggatcg atgccgcgca gtccgcgcgt gtccagcgtg ccgtcgggca
    20581 tgcgcacgac cgtcccgaag tcgacgtctt cgacctttag atcgactttg acgggctcgc
    20641 ccggtggggc cccggcgact ttggattgca gcgccgcgct tacttcgctg gccagcacct
    20701 gcaaaagtcc gccaccttgc agcgaaggcg gattgcgctc gagcgcactt ttgacccgct
    20761 caccatcccc accgacgagc gcattttcat tgtattcgcc cgcaccatcg tcaccaccgc
    20821 ggtgatgaca cgaccgacag ctaaacgagt ccggtccgcc tttggcccca cgatgcaccc
    20881 ggtgcatttt cggcccctgc ggcccccagg catgaccgaa gccgcgcacc accgccaact
    20941 cggtttcgaa cagctcctcg ccggcctgaa acagcgactc ggtcagttcc agttcttttt
    21001 tcgccgacgc tttgtgccgc gacgggttta ggtcatcgcg ctgttccgat ggttccaccg
    21061 ctgcgatgta tcgaccgatg cggcggacat cctggctttc cagcacatcg gccaaatagc
    21121 tctccagctc tgggcttagc gacatctgct gacccagcga cggcagcggc tgcgaagtcg
    21181 atgtctcaga aatcccggct gcgcttggcg gcccgctgtg acacgacccc agaaccaacg
    21241 cagccaagat catcgcccga ctgcgtaacc ggacagatgt gcgggaagac aaggaaactc
    21301 ctcggcacga tcttacttcg tcgggaaacg ccctcacaag cgcgaatttg tccgcgatgg
    21361 ccggctacag aaggcacacc gcggtcaaaa aaaaaccctc cgcttttggc ggagggcttg
    21421 tcggggcgac tggatttgaa ccagcgaccc ccagtccccc agactggtgc gctaaccagg
    21481 ctgcgccacg ccccgaaacg acgagagaca catctaccaa agcgccactg tccccgtcaa
    21541 gaaaactttg agaaaataag ttcccgtctc tgctgaaaaa gaacggggca accaaggacc
    21601 gactttccgt cgcgaccgcg gaagcagaac ggaaaaagtg aagaaagttc aaactttgat
    21661 ttggcggacc ggcaaacgcg atccatagac ctactatgtc cgacatatca ttcgatccgc
    21721 tcccctatct ccgtacctcc cctggcatga ccatcgagtc tggcatcttg ctcggccaga
    21781 cgctggtatc caccatgccc aaatccatgc ccgcccacgt aaagcgagcc gctgtaaagc
    21841 tcggcaaggt cgccgacgct gcacagaagg cgctcgcgag gcggcaaaaa gagctaggtc
    21901 agatccccga agaaactgcc cgcgacatcg acctcgccgc cgaccaagcc tggagcgcgc
    21961 tgcgtgacat cttggatgcg ctcagccgtt tgccatcgac ttatgaccgc gccaaaaaag
    22021 cgcggctgct ggtttcttca gtatttcccg aagggacagg ctttctgcgt ctgccctatt
    22081 ccgagcagta cgtgcagatg gacaccatct tgcgtcgcat cgacgaaagc ggactgacca
    22141 agagcattga tgcggtcgtc ggacccgaac tgctgctgga gatccgcgcc gtccatcttc
    22201 gctatggcgc gatgtgcacg cgacggctca atgacaaggg gccagccgac agcctgacct
    22261 ttcatctgcg cgcacttcag cggtcggtgg tcgagtatgc aacctctgtc gccagcaccg
    22321 tggacagcga tgatcctgca tcgatcgcgc tcgcactcga tgcgctgcgg cccatcgaca
    22381 atcatcgcca gcaggtcgcc agcgctcgcc gtcccgaatc cggaccgccc gccacgccag
    22441 atcccgcccc cagtcctccg cttgccccat aaaccaccga tccccaatca gccggtgaag
    22501 cgttcacccc caaaccgggg tgatgacgga atgggggtga agcattcacc ccgaaaccag
    22561 ggtcgttgct ggaatgaggg tgaagcattc accccgaaac cagggtcgct gctggaatga
    22621 gggtgaagcg ttcaccccca aaccggatgg ctggcggaat gggggtgaag atgtcactcc
    22681 gaaaccaggg tcgctgctgg aatgggggtg aagcgttcat ccccaaaccg ggcgctggtg
    22741 gattgggtca ggaagcgctg agcactgaac cgagccactg caccagatcg gaaaggcccg
    22801 atatcttgtg cagaatgcag cggcggactt tggcatgggt ctctttggcc acgccgctta
    22861 gcacgagatc ccgcgcaagc tggtctttgc acagcgcccg atcaaacagc tcatcggttg
    22921 gaccctcggc gcggtgttct ttgacacagt cacgcgccgc tttgagcacc gattccaggt
    22981 tgcgtgacag cacgaagacc tcgatgtgcg gtgccatgtc tgccgcacac agcgcgcgaa
    23041 tcgcctgggc aacctgcggc agtgtcgcgc atggctttag ccggagcttg tcgcggatca
    23101 cgtcttcatc gaccaccacc gccactttgc gtccatcgcg ggcgatgtcg ggcaagtctt
    23161 cgcggcaggt ccgcagtacc tggtcgacgc ctttttgaca gcgatgttcc agggcggctg
    23221 ccagctcgtg gtacggcgct gtgatggcat catcgtctcg caccgccgat atcaccagcc
    23281 gatgcagccc gaactcgttg cgctgtccac gcgcatcttc gtacaggatc gtgaccttgg
    23341 gcttgcggct catgctttgt ccgctgcggg ctcggcgtca gcccagatcg agtcatcgtg
    23401 accggcgctg cgcagatccc caatgccgcg atagcgtgcc agccagcggt cgagcgcttc
    23461 tttgtcgggg cgacggagct gcgtctggcc ttgctcatcc agctcgcaca gcacggcttg
    23521 gtccgccggg cgcgacaggc catcgagcag tcggtccgag tgcgtcgcaa tcagcaccgg
    23581 cgaggtctgg gaaagcgact ccagaaagtc gagcacccgc atgagcaggt gcgggtggag
    23641 gtgcagctcg ggctcgtcga acgcgagcag cgcggcgggc tggggacgcg gcgctcgata
    23701 cagcgccacc caggccagga acgccagcgt gccgtcggat agcgcaaacg tcggcaccgg
    23761 ctgaagctgc gaccgatact tgacccgcaa agatatcttg ccgccgtcgc ccccggctct
    23821 gaccagcaca tcttcgacgt ggtccccaag acccagccgg acaagctcca gcgtctcgcg
    23881 ccagtgggca tcgcccagct cgttgcgcag cgtgaaaaac gcgctgccga ggttgctgcc
    23941 aagcggctct aggcgctcga atggctcgac gatgttgtct tcgcgcatcg ccgaaggccg
    24001 cttcagactg cgactcgccc accagggcag cgtctcaaac ggcaaacaaa tctcaatgct
    24061 ttgaagtgcc cgagcgactt tgccgagcac actgattcgg tcgcggactt ggctgcgtgc
    24121 ggcgtcgtct agtccgttgg cgagcatctg ctcaaggttg ccggatcgct gtactcgatc
    24181 tgcaaaacca acaagcaact ccgtgtgagt tgggtgttcg ccgtaagtcc agtcggtgct
    24241 gcggggacgt tggttagggc ctgcgccaac cgtttctttg aaggtgagtt tgccttcgcg
    24301 gtcggccagg gcgaagtcgt agtcgatgac ttcgccgtct tcttcgatgc ggaccccgag
    24361 ctttagctgg tcggccccgt ggcgcagcag gccggaaaag ccccagtggg tgcggcggaa
    24421 ctctttgagg aagtcagggc tcggcgcttt gcgcaaaagc tcacaggctt cgatgatgct
    24481 gctcttgccg gtgccgtttg caccaatcag gaccgtaagc tcacgcaggc tcagccgcac
    24541 gtcggccagc gtgcgcaggc ccgcgatgcg gagttcggtg atcgccatag cgacgcatca
    24601 tacgcgccga cgtgacgttg ggatcggcaa aaacaggggg gatctagtct tcgtcgtcgc
    24661 cgaagaggat gccgatttgg agttcgagtg cgtcgaaggg agcggcgcgg acgcggtcgg
    24721 tgcggc
//
LOCUS       DAORGN010000054        24657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_796_length_24657_cov_6.463336, whole genome shotgun sequence.
ACCESSION   DAORGN010000054 DAORGN010000000
VERSION     DAORGN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 24657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24657
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_24657_cov_6.463336"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PKL17_08260"
     CDS             <1..1072
                     /locus_tag="PKL17_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK44760.1"
                     /translation="LLQSGLGDAPHDEAQRRALLRSLGQLPGVWVPSEHGEQLPKVAA
                     ADDDKLPATSAFLTPHTELSSMFLIEPERGCSRGCTYCVMRRSTNGGMRLVSPERIRS
                     LLPDGITRVGLVGAAVTDHPRLPQILDHVVNERGLQVGISSLRADRLTDEIVGLLAKG
                     GYRTLTVASDGASERMRDKIERRTKARHILRSAELAKQHGMKTLKVYMMVGLPDETDD
                     DIDELCAFAKEISKIIRLAYGVAPFVAKRNTPLDGAPFAGIAEVEQRLARLKSGLRGC
                     AEVRPTSARWAFVEYLLAQGGGDAGLRAYAAHLAGGSFAAWRRAFLGYATYHAPTQER
                     LKTMPDDGRLRLPLLQIGANPS"
     gene            complement(1069..1572)
                     /locus_tag="PKL17_08265"
     CDS             complement(1069..1572)
                     /locus_tag="PKL17_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HNK44761.1"
                     /translation="MTEPQPQLPPSLLIACYAEARRTYPREACGLLCGRRGAMAIDTL
                     RVCENDQDRLHALDPLGFPRDGRTAFRLRFVDVLWLYESLQTDVPARILFHSHVDVGA
                     AFSAQDRVEAQRHDVLRETFDHLVVDVSADGARGALRYRFVDGDFRCIARYDAQGRFL
                     QVDSGTS"
     gene            complement(1569..2339)
                     /locus_tag="PKL17_08270"
     CDS             complement(1569..2339)
                     /locus_tag="PKL17_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNK44762.1"
                     /translation="MTVPRLPGPLEIARLLYEHSARGDRGLIEVPTAKGNLRLQLSVG
                     FVLAMDVGPDAPVGSDSQVRYVLRARGLPQFRLGQTLSGRFAVEPFRPDASIRAHVDA
                     QALSAEKLRERIGLHKVTLSFPPHASALHSEERRVVARLSDALSLEELHKLGDLPPTR
                     LIRLLLFLDALGVLVIGLGRGELAAAYALLELPAQATRSEVKSAYRRLARTLHPDQFP
                     SADPAERRALADRFSAIHAAYRVLVTHLADEKKSPETA"
     gene            2455..2646
                     /gene="rpmE"
                     /locus_tag="PKL17_08275"
                     /pseudo
     CDS             2455..2646
                     /gene="rpmE"
                     /locus_tag="PKL17_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            2853..3926
                     /gene="prfA"
                     /locus_tag="PKL17_08280"
     CDS             2853..3926
                     /gene="prfA"
                     /locus_tag="PKL17_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNK44763.1"
                     /translation="MFDKLAEVENRYQDLEVQLADPDVLAKQQQFLKLAKERAEIEEL
                     VATYRGYKKTLDDIEQNAPLLRESDPDLRTMAREEEQRLLLLREETENKLKLLLLPKD
                     KNDDKNIVLEIRAGTGGEEAALFAGDLFRMYSRYAERKNWRVEILSMSEAAAGGLKEA
                     IVMISTQSSGKVYSELKYESGVHRVQRVPETEAQGRIHTSAATVAVLPEAEDVDVKIE
                     EKDLRIEVMRSGGPGGQSVNTTDSAVQIMHIPSGIIVRCQQEKSQHKNRATAMRILKT
                     KLLDIEREKQESAEREARRSQVKSGDRSEKIRTYNFPQDRVTDHRIGLTRHNLQKFMD
                     GDIQDVIDALRAHHQAELLKGET"
     gene            complement(3937..4455)
                     /locus_tag="PKL17_08285"
     CDS             complement(3937..4455)
                     /locus_tag="PKL17_08285"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44764.1"
                     /translation="MSTALDTNELMELASKVGPEEFSVRFPHLFLVFYETVDTVGVSS
                     FATQVISKDAARGRPTSQIRAVPLVKAPNNPYSDRISLGRARNCDVVLRNPSVSKLHA
                     HIRKEPNGSWVVIDANSHNGTTIDGNRVAAGQPIPIKIGEPITFGGMIVRIATATMVH
                     QALLGRPPWGSR"
     gene            complement(4559..7300)
                     /locus_tag="PKL17_08290"
     CDS             complement(4559..7300)
                     /locus_tag="PKL17_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44765.1"
                     /translation="MAIGNTDAQKERLWQSARTSEVDAVRRRLDSGQHVALVVGDGKS
                     GDGLGDPVIGRVLGEECGRLTSGQRRLGDVRLVVIDCGLYPQPLGALYEALRRVSELL
                     GTGRSGSQVAADRVLAGLRRRLMGDVGEPPPHLALLLALEKLGPKAGVSGVRWALAFA
                     RAERADADTLHELTQLVARRLSVPLLLLFGSSPTAGPGFDLLAALRACGGDDAVIECV
                     QAGHGDSGDTAKPRSLPALSPPVMAVLRALALTGAGCELSLLVELLGLRDDEVLWALQ
                     EASDAGVEIIDDGNDHFELKPELVSLLISPMLPSLVRSYHRRLAALLSDRLPPTNVLA
                     SATSATKTASPSATTAVLDSLPVPTLGSADTPDIGTEDVPAATTQPGAAPDVTPDAPG
                     PAPAGSEPPALANSAESEKDDPATQPRTTDREASTDSAASPALSQVLVASSEKTPAIP
                     AAPDPAAAPAAMSATVPLYADAMAPTLHWPYGAGPAAARTSSVGQSAPTPAGESPPAN
                     TKTAAAQNTAVAQNTATAPASDRSVRRAQPARAARHLIEAGEIDPGIDRYLHAVDQAL
                     RVGAIREALTLADRAMQLLGELPDSPLRRTLRIRALCAVGRIRWLGNGPDERFTLKGA
                     LTAFQEARALLRPTDSAALRTTVVSQLSAVLYEIGDLPSLEAALTELSDASRTLLGQG
                     EPAMAARLLNDQAAVYVRIGDPVRAAHLLEESRRFFSSGRFPSDDEEVARELADTEHL
                     LAKLPLHVAARQGRQLDAIGLGRSHAQQAAETYKRLGMRRELAHVTETLGRLETMAGR
                     HEAAMAYLSTAAELGRELHDLLLLARTTAAMSEVLVAANQLAEAIELLVESLSLNVEK
                     GSLIGLAFVRRSVEKLAQRVKITESSEAPTSRALARLYEDLLAAEDQLGRAKLPSDG"
     gene            7542..7970
                     /locus_tag="PKL17_08295"
     CDS             7542..7970
                     /locus_tag="PKL17_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNK44766.1"
                     /translation="MLNESQRSQLEKVLLGERERLLQAAKDGLKFSMERERSIGRDSI
                     DESMEEELFSTELRLRDREKFLLNKINAAIERLENRTIDECEDCGETISFRRLMARPV
                     TTLCIDCKEEKEKEELTKSQTIGRGGGGGDESMSDHMDDE"
     gene            8054..9415
                     /locus_tag="PKL17_08300"
     CDS             8054..9415
                     /locus_tag="PKL17_08300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44767.1"
                     /translation="MDSQRYKVTDRLDAGGMAEVFRGIAESSVGGLKRAVAIKRILPN
                     LTKNKRFVQMFLDEARLALHLQHTNIVLVYDVGSSETGDDTAYFLVMEFIDGCNLKVL
                     LQTLHTMGQRLPVSQTLYIILEVCKALAYAHDLADPETGKPLGIVHRDISPPNILFSK
                     RGEVKLADFGLAKAASQVENTDPGVVKGKFSYLSPEAARGEEVDRRADIFAVGILMYE
                     LLTGQRLFDGETDYATVKLVREAVVPSLIAQNPDVTPELETIVRRALARDRNQRYQRV
                     EELLEAIAHYLFSRGMKVTSRDIEQLIGRVLAERQKSKPQPQSTLIDNLIQDEILRFT
                     SLDEVGDEQQKSSEGQAPLNPDQFIDPRAWMAESQSSIPTRAPETPTSGPGLGKSVAP
                     LESLLAEKPALAVESAPATPPPSSVSAGFRSRKPSGPPLVPIIMAVVTVLLGILGYLL
                     LRN"
     gene            9489..10760
                     /gene="glgC"
                     /locus_tag="PKL17_08305"
     CDS             9489..10760
                     /gene="glgC"
                     /locus_tag="PKL17_08305"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNK44768.1"
                     /translation="MSPVFSPHRGPNPNVLALVLAGGEGRRLWPLTADRAKPSVPIGG
                     RYRLIDFVLSNLVNSGFLKIKVLTQYMSDSLNSHIARGWRLPAMLDQYVEPVPAQQRR
                     GREWFKGSADAIFQSLNVITDEDPDYVMVFGGDHVYKMDVQQMLDFHRDNSADCTVAC
                     IPVPVEQAKSFGIIEVDASWRILSFTEKPDCPREIPGRPGWALASMGNYIFNTRTLIQ
                     QIVLDAETTASSQHDFGRNILPDMVGKRALYAYDFMRNDIPGMDERERGYWRDVGTIQ
                     QYFLANLDFVQIVPVFNLYNPRWPLHTWVRSLPPAKFVFNEDGPGGQRRVGFATNSLV
                     SEGCIVSGGHVDQSVLGPRVRINSFAQVEQCILHESVDIGRHCRIKRAIIDKHVRVPA
                     GTEIGFDPEADRRRFQVIDDIVVIPKNFDFG"
     gene            complement(10874..11842)
                     /locus_tag="PKL17_08310"
     CDS             complement(10874..11842)
                     /locus_tag="PKL17_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNK44769.1"
                     /translation="MAEFLDKTPSCAYTHGLMSLFARRKPARKSGRSPRGVWLRALLF
                     WRPRNRRLGRAPVRTPRPEEKSTPRTALSVLLSGLTLVGLSALLVATVVTGRHYLLHS
                     PRFAVAAVEISPTHHVTTERLRSLSGVQLGQNLISLDTSDVAERLRREPWLRKIHVEK
                     RLPSQLKIVVSEFEPAALVALDSIYMITADGEVFRRAMPTDYDPKLPIVTGVGRAAYL
                     LDREWAQGQLTRALHVLSLYQQNPERPMVREAHIDQITGVTFSMADGSTIRLGQGDDS
                     ELTKRLQRFDAVWQTLKKKGETAQLVLLDNRAHPDHVTVRLSQKRP"
     gene            complement(11900..13141)
                     /locus_tag="PKL17_08315"
     CDS             complement(11900..13141)
                     /locus_tag="PKL17_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNK44770.1"
                     /translation="MQPRIYLDHSATTPLHPCVADAMRDALTLCGNPSSLHHEGRAAR
                     EAVESARRSVASLIGAQPAEVIFTSGGTESNNTALLSLAEVVRKLGQPLHAVTSPIEH
                     PSVQKSMQRLADRGFRVTQLAVDSFGRIDVQSFARLCEVDPPSLVSLSMVNHELGNIF
                     PVAELTQLAKQRGAWVHCDAVQGVSKLPISVRELGVDTLSVSAHKFYGPKGIGALFIS
                     GPQPGSCGTMPELPAFLVGGEQEKGRRAGTENVLAIVGFGRAAKLAQEELLWRVDELR
                     KRRERLESGLLRLPDVTVHGDPQLRSPTVCNARFAGVEGDMLLASLDLDGIAVSTGAA
                     CSSGSSEASKVLRGIGLPRDLARQAVRFSIGIGTTDDDIDRTLSCIAAAVGRIRALGP
                     SPLLSVPPKNQQTVQIRFSRP"
     gene            complement(13174..16446)
                     /locus_tag="PKL17_08320"
     CDS             complement(13174..16446)
                     /locus_tag="PKL17_08320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNK44771.1"
                     /translation="MSGKDSPYVELRTKSFFSFLQSTVDPEDLAKAAASAGYQAVGLI
                     DTASASGLARFHKAAKHEGIRPIVGGEVLVDGAPLALLCETAEGYRNLCRLITTQHSA
                     ARGGRGLTLFDVEQHHAGLIALSGGGDGKIGGLIDQGDADAAERKLDVLHGIFGAKNF
                     FVELQLHLDEREDRRNAVLTALADRKQLPLVLTNDVRMLGGDELLLLDALTCIREKVQ
                     LHAAGKRLLQNGERFLKPAREMAALLPDLPQAARNTVEIAGRCTFSLDALGYRFPLYP
                     LPAGETPEEFLRAVVFDAAESRFRPYEKRAKDQLERELGLIQKLGLCGYFLIVWDLIQ
                     FCKSRGILVQGRGSAANSAVCYALGITACDPLKMDLLFERFLSEERGEWPDIDLDFPS
                     GSRREEVIQYIYRKYGPAGCGLTATFTTYRARSAVRELGKILGFSDDSISQLAKKLSR
                     FGDEFGAYGSGIPEAEPTPFVEHVKSCGLNPEDRNVAQLVRLYEEIQDIPRHIGQHPG
                     GMVIAAGRLDEIVPIQPAAMPGRAIIQWDKDDVGDLGIVKIDLLGLGMMAVLEEASKM
                     VPYHDGQPFDMAALPKDDPQVYSLIARADTVGVFQIESRAQMSCLPRTRPAKFYDLVV
                     QVGLIRPGPIVGKMVHPYLRRCTGEEPVSYPHPSLAPILERTLGIPLFQEQVMRMAMV
                     AAGFSGGLADQLRRAMDSKRGTKKMRMLVDKLRAGMEAKGIPKDAAQQIEQAITAFAQ
                     YGFPESHAISFAWLTYASAYLKVHHPGVFYTALLNAWPMGFYAPATVIRDAQRQGVVI
                     RAIDVTASEWLCSIEHSPDREVGYWTADRPDNVIPFDRSPWHAAPHGVASAPVSRVLR
                     IGLRYVGGLKRSSAEAILKARLARPFVDLDDFMNRCRALSQEEISQLAELGALSSLQQ
                     KPSRREALWQVSKWEKQSELSLREGEQEDPSPLREMSQMEELQADLAGSGVTVGPHPI
                     ALLRRELDSYGVARIDELTKRSPKSRARVAGVCIIRQRPPTAKGFLFLTMEDETGLLN
                     VIVSPQLLAEQRTVIYGTRSLVIDGVIEKNGESVQLRADRFRPLDQVIAVISDAPSAN
                     TE"
     gene            complement(16459..17946)
                     /locus_tag="PKL17_08325"
     CDS             complement(16459..17946)
                     /locus_tag="PKL17_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HNK44772.1"
                     /translation="MALTCERVACLWIPSWPLQALLRARPELRQQKLVVCDGSLPSSQ
                     VLGATLAARQLGVRASQTVAQARSLCGELEVHELSEAHRQAAHDALYDVASSFSPSIE
                     CGDGYVLCGIGDMAKLFPDEKLLGKALLAQAESVRLHGRVAIASQAATAKLIARARAD
                     VTVVAPGDEKLYLRPLPLCVLPMSASQALLLSRFGLRTVGDFLALPRTKLPTRLGKDG
                     VALHRLAAGEDDSPLKPTPPPPEICESLDLDEPEANLEPLLFLLRGLTDRMMARLRAM
                     SLACGDFSVELLLKPQGVQRVEVKVATPTREVATLTELLRLSLQAHPPETEVRGLLVR
                     TVAQRPRPLQLGLFDGEQLLPERLMTLLSRLQALCGEGRVGQPMASDSHLPGLAEVVP
                     LKLPSRRDADEPPPSDPPQKMALHMLRPPRAATVKMDGIRLTHLHSDGLSGPVLRAGG
                     PYRIRGSAGNEGLRDYYDVELPSGEALRLFFDRDEGNWFLDARYD"
     gene            complement(17959..18699)
                     /locus_tag="PKL17_08330"
     CDS             complement(17959..18699)
                     /locus_tag="PKL17_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44773.1"
                     /translation="MFSSLARLSSVEDGDFQSVSLGSDSVDNRQAAIAKLRQACRSEV
                     QMSKAPPLPTGFAVIDESLGGGLPRGQIVEAVGKVGRLSLALSVLAKATQQKETAVFI
                     DGADALDPGGAMAQGIDLRRLLWARVKNGNSALRAADLTLGAGGVSLIVLYLVGVADA
                     QQLVRAALWSRLQHRAQRAKAALLVVTEKPLAQSFAVATLGFVDDGMRWQAAPGGRLQ
                     LCARQARIEVLRSRLGAPGDVQPFCLAR"
     gene            complement(18921..>22579)
                     /locus_tag="PKL17_08335"
     CDS             complement(18921..>22579)
                     /locus_tag="PKL17_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44774.1"
                     /translation="APAEPVLPPVSTEKAKPANEPGAACPKLEASRLLSAYFAIPDGD
                     KGRANHDAIHQALVSQDVSVQFLVATVPEPRLGFDGTVESITRALEATGFLLDRYLLP
                     WNPQADKRSGESSCSQDQPGVALFHRAATASGHGGSRRGLLLLYLVGESPIAGIHKPA
                     FISAIDDLLRIRALVPDGRCESCNEVRMVGPAFSGAVVSLDQILAHFPSLSFRMLSGR
                     ATSGAIRDQIQKWNDGMRSVRYQATVIPDEALQTEFFCFLTETLGADEHDVALISESS
                     TGYGQAVIAAQASQSQGLPPGYDRVFPAHKTHKSNHCKRAHRPRLTLPVPLHIARLHT
                     VDVAKTSPSTPRSSEPLLPDLVPSPVSRPLPTLLDDSPRTDIIPTLSPRTVSAAERSL
                     AHILSTIYAEKIRYVGILATDVQDKLFLARQIRAYSPDVVLFTFESDILYTHPEARSY
                     LKGMLVVSPYPLFTRNQQWSYPFRGLRQRIQFASDADQGVYNATVALLGYPEQLVEYS
                     QAAPGVDKPLSRPAIWVSAVGNDTLFPLAFIKDYDAGGYVYENPSIHRGQFEYRYSPY
                     QQGTLSFVMVLIGIFGLWLCAGYFVHYYFPDVTPPILLSPLKIFRRSSIVRSLLDDEL
                     AARESNRQPIFMLCLFVPAWVGYFVLTTTHFMQLRDGNLDELLDEKRLSLWLRFWYHS
                     GHIGVYQNLSWKVLCIALFGVVAQLAYSAALLDAMLLYLLPGWRSKLHDRFVAKWQGL
                     GSRWRRFWLTVVGLGVAGLLLLGFFLFVAFVNYLQVDFNHLLFLRRSAQPAFGLSPLM
                     PLLLLGLGVQLYGYCELQRIRLLEKLATVYLDLLEELEDDRGLRGKISEIAAYLGSPS
                     VWQILAGFLLALFQAVTVLSELVTLEVAPMHLVFRFLIALQVTMIGFVFYRFLRLWSV
                     FREFLHQLGHHPLAQSLERLPHKFIRSLGALFLEDLPELTRRQAAQEHFRLLINHMNQ
                     LDFDRDLLPLTTVTDIEKVKSLLQDLRELVPHIERETMQQGAATDVPEAQVVHASRIL
                     TQILKRFWRARPLPGSLVAGRPGEAQLSVRPGPEQNTAEVYQRLLPDTLHLWLRLAED
                     FLAIQVVTYINRLFPHLRNGLLNVTIGFVLLLLALIVYPFQPQRYLVLLSTVLLVMTV
                     PTTMYVLAQMNRDEVLSRIAKSQPGKLTWDRAFISQIVIYGVLPLLSLIATQFPEVRG
                     AAFSWLETALKTLK"
     assembly_gap    22580..22679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22902..24158)
                     /locus_tag="PKL17_08340"
     CDS             complement(22902..24158)
                     /locus_tag="PKL17_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNK44775.1"
                     /translation="MTGAHIARPAPVAASRPRYLALCARNTEPLVAAELVALPDVTDV
                     EVRQGAVSFSGPRAALYRANLYSRCASRILQVLGDYPCEGPADLYDAVRRLPWEEFID
                     PSGTMAVSAMGEGPGLANSMFTALKTKDAVVDRFRDRVGRRPDIDTQQPDLRINVQLY
                     SGARGTRMMIGADASDPPLFQRGYRQQGGLAPLKETLAAAIVAQTGEPALGATPISEA
                     VHTTPAHQPRPAEPRPSTIVDLCCGSGTLLTEAGLSVLRVAPGLSRRFGFMRWRDFDP
                     LLFRKLEQEAKAKQRAAAGVFLYGSDIDPRAVRMAGQNLAAAGLGRQSRLIRADLRSV
                     EPPPAQPGIVLCNPPYGERMGDEHELVDLYRALGDTLKRRFVGYRAYVYTANLQLARQ
                     IGLRPSARHVLWNGMLEGRLLRFDLY"
     gene            24272..24622
                     /locus_tag="PKL17_08345"
     CDS             24272..24622
                     /locus_tag="PKL17_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNK44776.1"
                     /translation="MSSDCIFCKIASRAIPSPLVYEDSEVVAFQDINPQAAHHVLIIP
                     RQHFADIGEAAAAGQSALLGQLVTAAAHIAKERGLSPSGYRLVCNTGKDGGQTVRHLH
                     FHLLGGRPLGWPPG"
BASE COUNT         5137 a         7923 c         7163 g         4334 t
ORIGIN      
        1 cctgcttcag tcgggcctcg gcgatgcgcc acatgatgaa gcgcagcggc gggcactttt
       61 gcgctcactt ggccaactgc ccggtgtttg ggtgcccagc gagcacggcg aacagcttcc
      121 caaagtcgcg gcggcagacg atgacaagct gcccgcaacg tcggcgtttc tgaccccgca
      181 caccgaactg tcgtcgatgt ttttgatcga acccgagcgg ggctgctcgc gcggatgtac
      241 ctattgtgtg atgcgtcggt cgacgaacgg cggcatgcgg ctggtatcgc ccgagcgcat
      301 tcggtcgctt ctgcccgacg gcatcacgcg cgtgggcctg gtcggcgcag ccgtgacgga
      361 tcatccccgg ctgccgcaga tcctcgatca cgtcgtcaat gaacgagggc tccaagtcgg
      421 catcagctcg ctgcgggctg atcggttgac cgacgaaatt gtcggactgc tcgccaaagg
      481 cggctatcgc acgctaaccg tggccagcga cggtgccagc gagcgaatgc gcgacaaaat
      541 cgaacgccgc accaaagccc gtcacatcct gcgatccgcc gagctggcca agcagcacgg
      601 catgaagacc ttaaaggtct acatgatggt cggtctgccc gatgaaaccg acgacgacat
      661 cgacgagctg tgcgcgttcg ccaaagaaat ctccaagatc atccgtttgg cgtatggcgt
      721 cgctccgttt gtcgccaagc gcaacacgcc gctcgacggt gcgccgtttg ctggcattgc
      781 cgaagtggaa cagcgtcttg cgcggctcaa gtcgggtctg cgtggctgtg ccgaggtacg
      841 tccgacttca gcgcgctggg cctttgtcga atatctgctc gcacaaggcg gcggcgatgc
      901 ggggctgcgt gcctatgccg cgcacttggc cggtggatca tttgcggcgt ggcgtcgggc
      961 gtttttgggg tatgcaacct accacgcacc gacgcaagaa cgcctgaaga cgatgccgga
     1021 tgacggtcgg ctgcgcttgc cgctgttgca gatcggcgca aacccaagct agctggtccc
     1081 gctgtccacc tgtaaaaagc gaccctgggc atcatatcgg gcgatgcagc gaaaatcgcc
     1141 gtcgacaaac cgatatctca gtgccccgcg tgcgccgtcc gctgacacat caacgaccag
     1201 gtgatcaaat gtttcgcgca gcacgtcgtg ccgctgggct tcgactcgat cttgggcaga
     1261 aaacgccgcg ccgacgtcga catgcgaatg gaacaagatg cgcgcaggca cgtccgtttg
     1321 cagcgattca taaagccaaa gtacatcgac aaaacgcagc cgaaacgcgg ttcgtccgtc
     1381 gcggggaaag cccagcggat ccagcgcatg cagccggtcc tgatcgtttt cacagacacg
     1441 caacgtatcg atcgccatcg caccgcgtcg cccgcagagc agaccacacg cttcgcgcgg
     1501 ataggtgcgc cgcgcttcgg cataacacgc gataagcagc gacggtggaa gctgcggctg
     1561 cggctcggtc atgcggtctc gggcgatttt ttttcatcgg ccaggtgggt gacaagcacg
     1621 cgataggccg catggatggc agaaaagcga tcggccagcg cccgccgctc ggcaggatcg
     1681 gcggagggaa attgatcggg gtgcagcgtc cgcgcgagcc gtcggtaggc acttttgact
     1741 tcactgcgcg tcgcctgggc cggcagctcg agcagcgcat acgccgccgc cagctcgccc
     1801 cgtccaagcc caatcaccag cacaccgagc gcgtccaaaa acagcagcaa ccgaatcagc
     1861 cgcgttggcg gaagatcccc cagcttgtgc agttcttcga gcgacagcgc atcggacagc
     1921 cgcgccacca ccctgcgttc ttcgctgtgc agcgccgagg catgcggcgg aaagctcagc
     1981 gtgactttgt gcagcccgat ccgctcgcgc agtttttctg ccgaaagcgc ctgcgcatcg
     2041 acatgggcgc gaatgctcgc atccggacga aacggttcca ccgcaaaacg gcccgaaagc
     2101 gtctgtccca gccgaaactg cggcagcccg cgcgcgcgca gaacatagcg gacctgcgaa
     2161 tcggaaccca cgggtgcatc tggcccgaca tccatcgcga gcacgaaacc gaccgacaac
     2221 tggagccgca aattgccttt tgccgtcggt acttcaatca atccacggtc tccgcgcgca
     2281 gagtgctcat acagcaaacg ggcgatctcg agcgggccag gcaggcgggg tacagtcacg
     2341 atcgtggcgg agtgtagcaa aggatcttgc acgccgcaga aagagaagat aagtaaatca
     2401 ctcttgtttt ggtagggcag ccctgcgccc gattgtttca aggaaaacct caccatggcc
     2461 aaagccggca ttcacccgaa ctatcaactc gccaccatca cctgcgcttg cggcgcggtg
     2521 tacgaaagcg cttcgacgcg cggctcgttt tcggtggaaa tctgcggtcg ctgccacccg
     2581 ttttacaccg gcaagcagaa gctgctcgac gccgcgggtc gcatcgatcg cttcaacaag
     2641 cgctaccagc taaagcccgg cgcggcgagc accagcatcg aagcgccgaa gaaggccgaa
     2701 gcccccaagg ccgaaaaaaa ggctgaagcc cccaaggccg aaaagaaggc tgaagccccc
     2761 aaggctgaca agaaggctga agcccccaag gctgacaaga agccgaaggc gtagttcccc
     2821 ttcttccctt tccttgctgc ccgcccatgt cgatgttcga caagctcgcc gaagtcgaga
     2881 atcgctatca ggacctcgag gtccagttgg ccgatcccga cgtcttggcc aaacaacagc
     2941 agttcctcaa gctggccaaa gagcgagctg aaatcgagga gcttgttgcc acctatcgtg
     3001 gttacaagaa aaccctcgac gacatcgagc aaaatgcacc gcttttgcgg gaatcagacc
     3061 ccgatttgcg gacgatggcg cgcgaggaag agcagcgact tttgttgctg cgcgaagaga
     3121 cggaaaacaa gctgaagctt ctgcttctgc ccaaggacaa gaacgacgac aaaaacatcg
     3181 tcctcgaaat ccgggcaggc acaggcggcg aagaagcagc gctgtttgcg ggcgacctgt
     3241 tccgcatgta cagccgctat gctgaacgca aaaactggcg cgtcgagatc ctgagcatgt
     3301 cggaagcggc tgccggcggt ctcaaagaag cgatcgtgat gatctcgacg cagtcaagcg
     3361 gcaaggtcta ttccgagctg aagtacgaat cgggcgtcca ccgcgttcag cgcgtgccgg
     3421 aaaccgaagc acaaggccgg attcacacct cggcagcgac ggtggcagtg cttccagaag
     3481 ccgaagacgt cgatgtgaaa atcgaggaaa aagacctccg catcgaagtc atgcgatcgg
     3541 gcggtccagg tggtcagtcg gtcaacacca ctgactctgc cgtccagatc atgcacatcc
     3601 cgagcggcat catcgttcgc tgtcagcagg aaaaatcgca gcacaaaaac cgtgcgacgg
     3661 cgatgcgcat cctcaagacc aagctgctcg acattgagcg cgaaaaacag gaatccgccg
     3721 agcgtgaagc acggcgttcc caggtcaaga gcggcgaccg atccgaaaag attcgcacct
     3781 acaacttccc gcaagaccgc gtcaccgatc accgcatcgg cctgacccgt cacaacctgc
     3841 agaagttcat ggacggcgac attcaggacg tgatcgacgc cctgcgcgcc caccaccaag
     3901 ccgagctgct caaaggcgag acctagtttt tgccagttag cggctgcccc aaggcgggcg
     3961 gccaagaagt gcctggtgca ccatcgtcgc ggtggcaatg cgaacaatca tgccgccgaa
     4021 ggtgatcggc tcaccgatct tgatggggat gggctggcca gcagcgacgc ggttgccgtc
     4081 gatggtggtg ccgttgtgtg agttggcgtc gatcacgacc cacgagccat ttggctcctt
     4141 gcggatgtgg gcgtgcagct tcgacaccga cggattgcgc aacacgacgt cgcagttgcg
     4201 ggctcgcccg agcgagatgc gatccgagta gggattgttg ggcgctttga cgagcggtac
     4261 ggcgcgaatc tggctcgttg gacgtccgcg ggcggcgtct ttcgagatga cctgcgtcgc
     4321 aaacgaggac acgccgaccg tatccaccgt ctcatagaag accaggaaga gatgcggaaa
     4381 ccgcacggaa aactcttccg ggcctacctt gctcgctagc tccatgagct cgttggtatc
     4441 gagagcggtg ctcatcaggc caacctaaaa agtggagtag aaaccagtct acgcgatcgg
     4501 cgcaaatagg ggaacgggct tggcaacttg gcgttgtcac ccacgctgcg tggctggttc
     4561 acccatcgga cggcagtttg gcccgaccga gctgatcctc ggcggccagc aggtcttcgt
     4621 acagccgtgc cagcgcccgc gaggtcggcg cttcgctgct ttcagtaatc tttacgcgct
     4681 gcgccagttt ttcaacgctg cgtcggacaa aggccagccc aatcagcgat cctttttcga
     4741 cattgagcga cagcgactcg accagaagct cgatagcctc tgccagctga tttgctgcga
     4801 cgagcacttc cgacatcgcc gccgtggttc gcgcgagcaa aagcagatcg tgcagctcgc
     4861 ggccaagctc cgctgccgtc gacaagtacg ccatcgccgc ttcgtgtcga ccagccatcg
     4921 tttccagccg tcccaaggtt tcggttacat gggccagttc gcgccgcatc cccagccgtt
     4981 tgtacgtctc ggcagcttgc tgcgcgtgcg atcgcccaag cccaatcgca tcaagctgcc
     5041 gtccctgccg cgccgccaca tgaagcggca gcttcgcgag caagtgctca gtgtcggcca
     5101 gctcccgcgc gacttcctcg tcatcggacg gaaagcgacc gctcgaaaag aagcgccgtg
     5161 attcttccaa aagatgcgcg gcccgcaccg gatcgccaat tcgcacgtag accgccgctt
     5221 gatcattgag cagccgcgcc gccatcgccg gttcgccctg gccaagcagc gtgcggctgg
     5281 catcggacag ctccgtcagc gccgcttcca gcgacggaag atcgccaatt tcgtaaagca
     5341 ccgccgatag ctgcgacacc accgtggtgc gcagcgccgc agaatccgtc ggacgcagca
     5401 gcgcgcgcgc ttcttggaac gccgtaagcg cccccttgag tgtgaaccgc tcgtcgggac
     5461 cattgcccag ccaacgaatc cgtccgaccg cgcacagcgc ccgaatccga agcgtgcgcc
     5521 gcagcgggga atccggcagt tcgccaagaa gctgcatcgc gcgatcggcc agcgtcagcg
     5581 cttcgcgaat cgccccgacg cgcagagctt gatcgaccgc gtggagatag cgatcaatcc
     5641 ctggatcgat ttcgcccgct tcaatgaggt gccgggctgc tcgcgcgggt tgggcccgtc
     5701 ggaccgatcg atctgaggcg ggggccgtcg cggtattttg cgccaccgcg gtattttgcg
     5761 ccgccgcggt cttagtattc gcaggtggac tttcgcccgc tggggtcggt gcggattgac
     5821 cgaccgatga cgtccgggcc gccgccggac ccgcgccgta tggccaatgc agcgtcggag
     5881 ccatcgcatc agcatacagt ggaaccgtcg ccgacatcgc cgccggagca gccgccggat
     5941 caggtgccgc cgggatggct ggcgtttttt ccgacgaagc aaccaacact tgcgatagcg
     6001 ctggcgatgc agcgctgtcc gtcgaagcct cgcgatcggt cgtccgtggc tgggtcgccg
     6061 gatcgtcttt ttctgattct gccgaattcg caagtgctgg cggttccgaa cccgcgggtg
     6121 caggccccgg agcgtcgggc gtcacatccg gtgcggcacc gggctgggtc gtcgcagcgg
     6181 gcacatcttc tgtgccaatg tccggggtgt ccgccgatcc cagcgtcggc accggcaatg
     6241 aatccagcac cgcggtcgtc gccgacggag acgccgtctt tgtcgccgat gttgcgctgg
     6301 ccagcacgtt cgttggtggc aaacggtccg aaagcagcgc cgccagccgg cgatggtagc
     6361 tacggaccag cgacggaagc attggggaaa tcaacagcga aaccagctcg ggctttagct
     6421 caaagtgatc gtttccgtcg tcaatgatct cgacgcccgc atcggacgcc tcttgcagcg
     6481 cccacagcac ttcatcgtcc cgaagcccga gcagttcgac gagcagcgac agctcacagc
     6541 ccgcaccggt gagcgcgagc gcccgcaaca ccgccatcac cggcggcgac agtgccggca
     6601 gcgaacgcgg ctttgcggtg tcgcccgaat caccatgtcc cgcttggaca cactcgatca
     6661 ccgcatcgtc ccctccgcat gcacgcagcg cagccagcag gtcaaacccc ggccccgctg
     6721 tcggcgacga accgaacagc agcagcagcg ggaccgacag ccgccgcgcc acaagctggg
     6781 tcagttcgtg cagggtatcg gcgtcggcgc gctccgcccg agcaaaggcg agcgcccagc
     6841 gaaccccgct gacacccgct ttcggcccga gtttttcaag cgccagcagc agcgccaggt
     6901 gcggtggcgg ttcccccaca tcgcccatca gccgacgccg cagtcccgcc agcacccgat
     6961 cggctgcgac ctgcgaaccc gatcgccccg tgccaagcag ctcgctgacc cgacgcagtg
     7021 cctcatagag cgcaccgagc ggctgtggat acagcccaca gtcaatcacc accagccgca
     7081 catccccaag tcggcgctgc cccgatgtca gccgcccaca ttcctccccg agtacacgcc
     7141 cgatgaccgg gtcgccaagc ccgtcccccg atttgccatc cccgaccaca agcgccacat
     7201 gctgccccga atcaagccgc cgacgcacag catcgacttc gcttgtacgc gcgctttgcc
     7261 agagccgctc tttttgcgca tccgtgttcc cgatcgccat ccaaaaacct cccggctggt
     7321 gctcaaaata aaccaaactc tgtccgaaaa ctgaaggcgc caggccggcc tggctgtgtc
     7381 gaaatccccc gttgacaagc ggcggcgcgg acctatagag tacgctacct tatgacattt
     7441 agctggctcg cgggtgcctc gacggtgaag gcagacgccc aacaagtaca cagagccagc
     7501 aagccggatt ttttcagacg ggtaagaagg ggttcgcaca catgctgaac gaaagccagc
     7561 ggtcgcaact tgagaaagtg cttctcggag agcgcgagcg cttgttacag gcagcgaaag
     7621 atggtctcaa gttcagcatg gagcgcgagc gcagcattgg tcgtgactcc attgatgaat
     7681 cgatggagga agagctgttc tcgacggagc ttcggcttcg cgatcgcgaa aagttccttc
     7741 tgaacaagat caacgctgcg atcgagcggc tggaaaatcg caccattgat gaatgcgaag
     7801 actgcggcga aacgatttcg ttccggcggc tgatggcacg cccggtgacg actttgtgca
     7861 tcgactgcaa agaagaaaaa gaaaaagaag agctgaccaa gagtcagacg atcggtcgtg
     7921 gtggtggcgg cggcgatgag tcgatgagcg accacatgga cgatgagtga tagacttggg
     7981 tagttacgcg ctcagcgcga cgttctaaac ccaccaaaat ggccttttag gcctttgcaa
     8041 gttaggtcag cagatggatt cccagcgcta taaagtcacg gaccgcctcg atgcaggcgg
     8101 aatggcagag gtctttcgcg gcattgccga gtcctcggtc ggtggcctca agcgcgcggt
     8161 cgcgatcaag cgaatcctgc cgaacctgac caaaaacaag cgcttcgtgc agatgtttct
     8221 cgacgaagcg cgcctggcac tgcaccttca gcacaccaac attgtgctgg tgtacgacgt
     8281 cggctcctcg gaaacgggcg atgacaccgc ctacttcctg gtgatggagt tcatcgacgg
     8341 atgcaacctc aaggtgcttt tgcagacgct gcacacgatg ggtcagcgac tgccggtcag
     8401 ccagacgctg tacatcatcc tagaggtctg caaagcgctg gcgtatgcac acgacctggc
     8461 tgatcccgaa acgggcaagc cgctcggcat cgtgcaccgc gatatctcgc cgccgaacat
     8521 ccttttttcg aagcgtggcg aggtcaagct ggccgacttt ggtctggcca aagcagcgtc
     8581 acaggtcgaa aacaccgatc cgggcgtcgt caaaggcaag ttcagttatc tgtcgccgga
     8641 agcagcgcgc ggcgaagaag tcgatcgtag ggccgatatt tttgccgtcg gcatcctgat
     8701 gtacgaactt ttgaccggcc agcgtctgtt cgatggtgaa accgactatg cgacggtgaa
     8761 gctggttcgt gaagcggttg tgccgtcgct tatcgcgcaa aatcccgacg tcacgcccga
     8821 actggaaacc atcgtgcgcc gggcgctcgc gcgcgatcgc aaccagcgct atcagcgcgt
     8881 cgaagagctg ctcgaagcga tcgcccacta tctgttttcg cgcggcatga aggtcacgag
     8941 ccgcgacatc gagcagctca tcggtcgggt gctcgccgag cggcagaagt cgaagccgca
     9001 gccgcagagc acgctcatcg acaacctgat ccaagacgaa atcctgcgct tcacttcgct
     9061 cgacgaagtg ggggacgagc agcaaaaatc cagcgaagga caggcgccgc tcaatcccga
     9121 tcagttcatc gatcctcgcg cgtggatggc cgaaagtcag tcgagcattc cgacccgcgc
     9181 accggaaaca ccgacgtccg gtcctgggct tggcaagagc gtcgcgccgc tcgagtcgct
     9241 gctggccgaa aagcccgccc tggctgtgga atccgccccg gccacgccac cgccgtcttc
     9301 ggtttctgct ggatttcgtt cacgaaaacc gagcggtccg ccgctggtcc cgattatcat
     9361 ggcagtagta acagtgctct tgggcatctt ggggtatctg ctgctgcgaa actgagtgct
     9421 cgttgtcttc actcggtgcg cagccaggac caaagatgtg tccttgactg aacctggatg
     9481 gagacggcat gagcccggtg ttttccccgc atcgcggacc gaatcccaac gtcctggcgc
     9541 tcgttttggc gggtggcgaa ggacgacgtc tgtggccgct gaccgccgat cgcgccaaac
     9601 cgtcggtgcc gatcggtggc cgctatcgcc tgatcgactt cgtcctgtcg aacctggtca
     9661 actccggttt tctcaaaatc aaagtgctga cccagtacat gagcgattcg ctcaacagcc
     9721 acatcgcgcg cggctggcgg ctgccggcga tgctcgatca gtacgtggag cccgtccccg
     9781 cacagcagcg acggggccgc gagtggttca agggttctgc cgacgccatc ttccagtcgc
     9841 taaacgtcat caccgacgaa gatcccgact acgtgatggt gttcggcggc gaccatgtct
     9901 acaaaatgga cgtccagcag atgctcgact tccatcgcga caacagcgcc gactgcaccg
     9961 tcgcctgcat cccagttccc gtcgagcaag ccaagtcgtt cggaatcatc gaagtcgacg
    10021 ccagctggcg aatcctgtcg tttactgaaa aacccgactg tccgcgcgaa atcccaggcc
    10081 ggcctggctg ggcactcgcg tcgatgggca actacatttt caacacccgg acgctgattc
    10141 agcagatcgt cctcgacgct gaaacaaccg cgagcagcca gcacgacttc ggtcgcaaca
    10201 tcctgcccga catggtcggc aagcgtgcgc tgtacgccta cgacttcatg cgcaacgaca
    10261 tccccggcat ggacgaacgc gagcgaggtt attggcgcga cgtcgggacg atccagcagt
    10321 actttttggc caacttggac tttgtgcaga tcgtgccggt gttcaacctc tataacccac
    10381 gctggccgct gcacacctgg gtgcgatcgc tgccaccggc caagttcgtc ttcaacgaag
    10441 acggtcccgg tggccagcgc cgcgtcggtt ttgcgacaaa ctcgttggtc agtgaaggct
    10501 gcatcgtgtc gggcggccac gtcgaccaga gcgtgctcgg cccgcgcgtc cgcatcaaca
    10561 gcttcgccca ggtcgagcag tgcatcctgc acgaatccgt cgacattggc cggcactgcc
    10621 gcataaagcg cgccatcatc gacaagcacg tccgcgttcc cgccggcacc gaaatcggct
    10681 tcgatccgga agccgaccgc cgccgcttcc aggtcatcga cgacatcgtc gtcatcccca
    10741 aaaacttcga cttcggctag ccaacgcgca caagcggtgg ccatcgatgc gctgccgcac
    10801 cacacatgcc gctccgcgca cgacgagcac ccacgacccc gcccaatcgc gacctgtggg
    10861 aaggtcgaac gatctacgga cgcttttggg acagtcgcac ggtgacgtgg tcgggatggg
    10921 cgcggttgtc gagaagcaca agctgcgcgg tttccccttt tttcttgagc gtctgccaca
    10981 ccgcatcaaa gcgctggagc cgttttgtaa gctcgctgtc gtcgccctgc ccgagccgaa
    11041 tcgtcgagcc atcggccatc gaaaacgtaa cgccggtgat ctgatcgatg tgggcctcgc
    11101 gcaccatcgg gcgttcgggg ttttgctgat acagcgacag cacatggagc gcccgcgtaa
    11161 gctgaccttg cgcccactct cggtcgagca gatacgccgc ccgacccacc ccggtcacaa
    11221 tcggcagttt cggatcatag tccgtcggca tcgcccgtcg gaagacctca ccgtcggcag
    11281 tgatcatgta gatcgaatca agcgcaacca gcgccgcggg ctcaaactca ctcacgacga
    11341 tttttagctg cgacggcaga cgtttttcga cgtgaatctt gcgcagccaa ggctcgcgcc
    11401 gaagccgctc cgccacgtcg ctggtatcga gcgaaatcag gttctgccca agctgaaccc
    11461 ccgacaagct gcgcagtcgt tcggtggtga catgatgcgt gggtgaaatt tcgaccgcag
    11521 cgacggcaaa tcgaggactg tgcaaaagat agtgccgtcc cgtcaccacc gtcgcgacca
    11581 aaagtgccga cagcccgacc aaagtaagcc cagacagcag caccgacaac gcagtccgcg
    11641 gcgtcgattt ttcctcggga cgcggcgtcc gcaccggcgc tcgtccgagc cgacgattgc
    11701 gcggtcgcca gaacaagagc gcccgcagcc acactccgcg aggactgcga ccggactttc
    11761 gcgccggttt ccgacgagca aacagcgaca taagcccgtg agtgtaggcg cagcttgggg
    11821 tcttgtccaa aaactcagcc aactgcgaac cgagccgagc gacggacagc tttaatttgg
    11881 gcggcggaag attccggtgt cacggacggg aaaagcggat ctgcacggtc tgttggtttt
    11941 tgggcggcac agacagaagc ggactcggtc cgagtgctcg gatgcggcca actgccgcgg
    12001 cgatacagga caaggtgcga tcgatgtcgt cgtcggtcgt cccaattcca atcgaaaaac
    12061 gcacggcctg gcgtgccaga tcccgcggaa gaccaatccc gcgcagcact ttcgacgcct
    12121 cgctggaccc tgaactacac gccgcgcccg tcgaaaccgc aatcccatcc aagtcgagac
    12181 tggccagcag catgtcgcct tcgaccccgg caaagcgcgc attgcacacc gtcggactac
    12241 gaagctgcgg atcgccgtga accgtcacat caggcagtcg caagagccca ctctccagtc
    12301 gctcacgtcg cttgcgcagc tcatcgaccc gccaaagcag ctcttcctgg gccagcttgg
    12361 cagcgcgacc aaaacccacg atcgccagca cgttttcggt tcctgcccgc cgcccttttt
    12421 cctgttcacc accgacgaga aatgccggaa gttcaggcat cgttccgcag cttcccggct
    12481 ggggcccaga aatgaacagc gcgccgatcc ctttgggtcc gtaaaacttg tgcgccgaca
    12541 cgctcagggt atcgacgccc agctcacgca ctgaaatcgg cagcttgctc accccctgca
    12601 ccgcgtcaca gtgaacccat gccccccgct gcttggcgag ctgcgtcagt tccgccaccg
    12661 ggaatatgtt gcccagttca tggttcacca tcgacagcga cacaagcgac ggcggatcga
    12721 cttcacacag ccgagcaaac gactgcacat cgattcggcc aaacgaatcg acggcaagct
    12781 gcgtgacccg aaaaccacga tctgcaaggc gctgcatcga cttttgcacc gacggatgtt
    12841 caatcggcga agtcaccgca tgcagcggtt gccccagctt gcgcaccact tcagccagtg
    12901 aaagcagcgc ggtgttgttg gactcagtcc cacctgacgt gaagatcact tccgcgggct
    12961 gcgcaccaat aagcgacgcg acgctgcgac gggccgattc aacggcctcg cgcgcggctc
    13021 gcccctcgtg atgaagcgaa gatggattgc cacagagcgt cagcgcatcg cgcatcgcat
    13081 cggccacaca aggatgaagc ggcgtcgttg cgctgtgatc gagataaatc cttggctgca
    13141 tgtgcgaaac ctactcgcag ccgcgccgct tcgctactcg gtatttgcgc tgggcgcatc
    13201 ggaaatcact gcgatcactt gatcgagagg ccgaaagcga tcggcgcgaa gctgtaccga
    13261 ttcaccattt ttttcgatca ccccgtcgat gaccaagctg cgcgtgccgt aaatcacggt
    13321 gcgctgctcg gccaaaagct gcggactgac gatgacgttg agcagcccgg tttcatcttc
    13381 catcgtcaaa aacagaaagc ctttggccgt cggtggtcgc tgacgaatga tgcagacgcc
    13441 ggccacgcga gcgcgacttt tcggactgcg tttggtcagt tcatcgatcc gtgccacccc
    13501 ataggaatcg agctcgcgcc gcagaagcgc aatcggatgc ggtccgaccg tgacaccact
    13561 gcccgccagg tccgcttgca gctcctccat ctgcgacatc tcgcgcagcg gcgatggatc
    13621 ttcctgttcc ccttcgcgca aagacagctc gctctgcttt tcccatttgc tgacttgcca
    13681 cagtgcttcg cggcgactcg gcttctgctg aagcgacgac agcgcaccca gctcggcaag
    13741 ctgcgaaatc tcttcctgcg acagcgcgcg acagcgattc atgaagtcat ccagatcgac
    13801 aaacggacgc gccaaacggg ctttcagaat cgcttctgcc gatgagcgct tcagtccgcc
    13861 gacataacgc agaccgatgc gtagcacacg cgaaaccgga gcagaagcga ccccgtgcgg
    13921 agccgcatgc cacggactcc tatcaaacgg aatcacgttg tcgggtcgat ccgcagtcca
    13981 atatccaacc tcgcgatcgg gactgtgttc aatggaacag agccactctg aagccgtgac
    14041 atcgatcgcg cggatcacca caccttgccg ttgtgcatcg cgaatcactg tcgctggcgc
    14101 ataaaatccc atcggccatg cattgagcag cgcagtataa aacacacccg gatgatgcac
    14161 tttcagatac gcgctcgcat acgtaagcca tgcaaagctg atcgcgtgcg attccggaaa
    14221 tccgtactgt gcaaacgcgg tgatcgcttg ttcaatctgc tgggctgcgt ctttgggaat
    14281 ccccttcgct tccattccgg ctcgcagttt gtcgacgagc atgcgcatct ttttggtacc
    14341 acgcttactg tccatggcac gccgcagctg atcggccaga ccgcccgaaa aacctgccgc
    14401 aaccattgcc atgcgcatca cttgttcttg aaacagcggg attcccagcg tccgctctag
    14461 gatcggtgca agcgaaggat gcggatagga aaccggttct tccccggtac agcgccgaag
    14521 gtacggatga accatctttc cgacgatggg accaggccgg atcagaccca cttggaccac
    14581 cagatcatag aattttgccg gacgagtgcg cggcagacaa gacatctgcg cgcggctttc
    14641 gatctgaaaa acccccactg tatcggctcg cgcaatcagc gaatagacct gtggatcgtc
    14701 tttgggaagc gcagccatat caaaaggctg tccgtcgtga tacggcacca ttttgctcgc
    14761 ttcttccagc accgccatca ttcccaaacc cagcagatcg atcttcacaa tccccagatc
    14821 gccgacatca tctttgtccc actgaatgat cgcgcgtccg ggcatcgcgg caggctgaat
    14881 cggtacaatc tcgtcgagcc gaccggcagc gatgaccatt ccaccgggat gctgtccgat
    14941 gtggcgcggg atgtcttgga tctcttcata cagccggaca agctgggcaa cgttgcggtc
    15001 ttctggattg agtccgcagc ttttcacatg ttcaacaaaa ggagtcggct cagcttcggg
    15061 gattcctgaa ccatacgccc cgaattcatc gccaaatcga ctcagctttt tcgcgagctg
    15121 cgaaatagaa tcatctgaaa agcctaatat tttgccaagc tcgcgcacgg cactgcgcgc
    15181 ccgataggtg gtgaacgtcg ccgtcagtcc gcagccggct ggaccgtatt tgcgatagat
    15241 gtactgaatg acttcttcac gccgacttcc cgacggaaaa tccaggtcta tgtccggcca
    15301 ctcgccccgt tcttccgaca aaaaacgttc gaacagcaga tccattttca gcggatcgca
    15361 cgccgtgatt cccagtgcgt aacagaccgc agagttggct gcgctgccgc gaccctgcac
    15421 caagattccg cgacttttgc aaaactgaat cagatcccag acgatcaaaa agtagccgca
    15481 aagtcccagc ttttggataa gtcccagctc gcgttcgagc tgatctttgg cgcgcttttc
    15541 gtagggacgg aatcggctct cggcagcgtc gaaaaccacc gcgcgcagga actcttccgg
    15601 ggtttcgcca gccggcagcg gatacagcgg aaaacgatag ccgagcgcat ccaaactgaa
    15661 cgtacagcga cccgcaattt cgacagtatt tcgcgccgct tgcggcagat cgggaagcag
    15721 cgccgccatc tcacgcgcgg gctttaggaa tcgttcgccg ttttgcaaaa gccgctttcc
    15781 cgccgcgtga agctgcactt tttcgcgaat gcacgtcagc gcatccagca aaagcagctc
    15841 atcgccaccg agcatccgca catcattggt cagcacaagc ggcagctgtt tgcgatcagc
    15901 aagcgcggtc agcactgcgt ttcgccgatc ctcgcgttca tccaagtgaa gctgtagttc
    15961 gacgaagaag ttcttcgcgc caaagattcc gtgcagcaca tcaagcttgc gctctgccgc
    16021 atccgcatcg ccctggtcga tcagtccacc gatttttccg tcgccgccac cgctcagcgc
    16081 aatgagcccc gcgtgatgct gttccacatc aaacagcgtc agtccccgac ccccgcgcgc
    16141 cgcactgtgc tgcgtcgtga tcagccgaca caggttgcga tagccttccg cggtttcaca
    16201 caagagtgca agcggcgcac cgtcgaccag aacctctcca ccgacaatcg gacggatgcc
    16261 ttcgtgcttg gcggctttgt gaaaacgcgc aagtcccgac gcgctcgctg tgtcgataag
    16321 tccgaccgct tgatagcccg ccgaggccgc cgctttggcc aagtcttcgg gatcaaccgt
    16381 gctctgcaaa aagctaaaaa agctcttggt ccgcagctcg acgtaaggac tgtccttgcc
    16441 gctcatgccc acacctcgtt agtcgtagcg cgcgtcgagg aaccagtttc cctcgtcgcg
    16501 atcaaagaac agccgcagtg cctcgccgga aggcagctcg acgtcgtagt agtcgcgcag
    16561 tccttcattt ccggcgctgc cgcggatgcg atagggtccg cctgcgcgca ggacggggcc
    16621 gctcagtccg tcgctgtgca ggtgggttaa ccgaatgcca tccatcttga cggtggcagc
    16681 tcgtggtggt cgcagcatgt gcagcgccat cttttgcggc gggtctgacg gtggcggttc
    16741 gtcggcatcg cgccgcgacg gcagcttgag cggcaccact tccgcaagcc ccggtagatg
    16801 cgaatccgac gccatcggct gccccactcg cccttcgcca cacagcgctt gcagccgcga
    16861 aagcaacgtc atcaaacgct cgggaagtag ctgttctccg tcgaacagcc cgagctgaag
    16921 cggcctgggt cgctgcgcca ccgtccgcac caaaagacca cgcacctcgg tttccggcgg
    16981 atgcgcttgc agtgacagcc gcagaagctc ggtcagcgtc gccacttcgc gcgtcggtgt
    17041 cgccaccttg acttcgacgc gctggacgcc ttgcggtttt agcaaaagct cgacggaaaa
    17101 atcgccgcac gcaaggctca tcgcccgcag ccgcgccatc atccggtcgg tcagaccgcg
    17161 caaaagaaac agcagcggct ccaagttcgc ttcgggctcg tccagatcca gcgattcaca
    17221 aatttctggc ggcggcggcg tcggcttgag cggcgagtca tcttcccccg cggccagccg
    17281 atgcagcgcc accccgtctt tgccaagccg cgtcggcagt ttcgtccgcg gcagcgccaa
    17341 aaagtccccc accgtccgca gtccaaagcg cgacaaaagc agcgcctgcg aagccgacat
    17401 cggcagcaca cagagcggaa gcggacgcag gtacagtttt tcgtcgcctg gcgccaccac
    17461 cgtgacatcg gcacgcgctc gcgcaatcag ttttgccgtc gcggcttggc tggcaatggc
    17521 gacccgaccg tgcagccgca ccgattccgc ctgcgcgagc agcgccttgc ccagaagctt
    17581 ttcgtcggga aacagcttgg ccatgtcgcc aatgccgcac agcacgtaac cgtccccaca
    17641 ctcgatgctg ggggaaaacg acgacgccac gtcgtacaag gcgtcgtgcg ccgcttgccg
    17701 atgcgcttcg ctaagctcgt gcacttccag ctcgccacac agcgagcgcg cctgcgccac
    17761 cgtctgagac gcccgcaccc caagctgcct ggccgcaagc gtcgcgccca gcacttgcga
    17821 cgacggcagc gagccgtcgc agacgaccaa tttctgctgt cgcagctctg gtcgcgcccg
    17881 caaaagcgct tgcagcggcc acgatggaat ccacaagcag gcgacacgtt cgcaggtcag
    17941 cgccatcaca gacccccact acctggccag gcaaaatggc tgcacgtcgc cgggcgcccc
    18001 gagccgactg cgcaacactt cgatgcgggc ttgccgcgca cagagctgaa gccgtccgcc
    18061 cggcgccgct tgccagcgca tcccgtcatc gacaaacccg agcgtcgcaa ccgcaaagct
    18121 ctgcgcgagc ggcttttccg tcaccaccaa aagcgccgcc ttggcccgct gcgccctgtg
    18181 ctgaagccgc gaccacagcg ccgcccgcac cagctgctgc gcatccgcaa ccccgaccag
    18241 gtaaagcacg atcagcgata ccccaccggc ccccagcgtc aggtcggcag ctcgcagcgc
    18301 agaattacca tttttcaccc gagcccaaag cagacgccgc agatcgattc cttgcgccat
    18361 cgcgccccct ggatcgagcg catcggcgcc atcaatgaac accgcggttt ctttttgctg
    18421 ggtcgctttg gccagcaccg acagcgccag cgacagccgc ccgaccttgc ccaccgcttc
    18481 gacgatctgt ccgcgcggca gtccaccacc gagcgattca tcgatcaccg caaaacccgt
    18541 cggcagcggc ggcgctttgc tcatctgcac ttcgctgcga caggcctgcc ggagcttggc
    18601 gattgcagct tggcgattgt cgacggagtc agatcccagc gacacgcttt ggaagtcgcc
    18661 gtcttctacc gagctcagcc gcgccagact gctaaacatg cgttcaggct attcggatca
    18721 aatgttcagg tcaatcaatt tgagaagaat tttacccaaa gtggttttct tagggaaaat
    18781 tcaaagtgaa ataagctgct cagcgacgaa ggttgtgttc gccagccgat ggttgggcgc
    18841 agagcggacg cagccgttcc cacgacccac gccgcgcgat gcagcgcgac ggggcttgac
    18901 ggtttttgct gacgagttcg ttatttcagg gtcttgagcg cggtttcgag ccacgaaaaa
    18961 gcggcaccgc gtacttcggg gaactgggtc gcaatcaagc tgagcagcgg cagcacaccg
    19021 tagatcacga tctgggaaat aaatgcacgg tcccaggtca gcttgccggg ctggcttttg
    19081 gcgatgcgcg acagcacttc gtcgcggttc atctgcgcca gcacgtacat cgtcgtcggc
    19141 accgtcatca ccagcagcac cgtcgacaga agcaccagat accgctgcgg ctgaaacgga
    19201 tagacgatga gcgcgagcaa aagcagcaca aacccgatcg tcacgttgag cagtccgttg
    19261 cgcagatggg gaaacagccg gttgatgtag gtcacgacct ggatcgccaa aaagtcctcg
    19321 gcgagccgca gccacaggtg cagcgtgtcg ggcaagagcc gctggtagac ttcggcggtg
    19381 ttctgttcgg ggccgggacg cacgctgagc tgcgcttcac cgggccgacc ggcgacgagc
    19441 gaaccgggca gaggccgcgc ccgccaaaac cgcttgagga tctgggtcaa aatccgcgac
    19501 gcatgcacga cttgggcttc gggcacatcc gtcgcggcgc cttgctgcat cgtctcacgc
    19561 tcgatgtggg gcacaagctc gcgcaggtct tgcagaagcg acttgacctt ttcgatgtcc
    19621 gtcaccgtcg tcagcggcag cagatcgcga tcgaaatcga gctggttcat gtggttgata
    19681 agcaggcgga agtgttcctg cgcggcctgt cgacgggtca gctctggcag gtcttccaaa
    19741 aacagcgccc caaggctgcg aatgaacttg tggggcaagc gctcgagcga ctgggcgagc
    19801 gggtgatgcc caagctgatg cagaaattcg cgaaacactg accacaggcg caagaaacga
    19861 taaaaaacaa agccgatcat cgtgacttgc agcgcgatca gaaaacggaa cacaaggtgc
    19921 atcggcgcga cctcgagcgt caccagttcc gacagcaccg tcaccgcttg aaacagcgcc
    19981 aagagaaagc cggccaaaat ctgccacacc gacggacttc ccagataggc ggcgatctcg
    20041 gatatcttgc cgcgcagacc gcggtcgtct tcaagctcct cgagcaaatc gagatacacc
    20101 gtcgccaatt tttcgagcag gcgaatccgc tgtagctcgc agtagccgta cagctgcacg
    20161 ccaagcccga gcaaaagcag cggcatcaag ggagacagtc caaaggccgg ctgcgcgctc
    20221 cgtcgcagaa acagcaagtg attgaagtcg acttgcaggt aattgacaaa agcgacgaac
    20281 aagaaaaaac caagcagcaa aaggccggcc acaccaagcc caaccaccgt cagccaaaac
    20341 cttcgccagc gactgccgag accctgccac tttgcgacaa agcgatcgtg cagtttgctg
    20401 cgccagcccg gcaaaaggta aagcagcatc gcgtcgagca gcgccgccga atacgcaagc
    20461 tgcgccacca ccccaaacag cgcaatgcac agcactttcc acgacaggtt ttggtaaacg
    20521 ccgatgtggc ccgagtgata ccaaaaccgc agccagaggg acaggcgctt ttcgtcgagc
    20581 agctcgtcga gattgccgtc gcgaagctgc ataaagtgcg tcgtcgtcag cacgaaatag
    20641 ccaacccacg ccggcacgaa cagacacagc ataaaaatcg gctgccggtt cgattcgcgc
    20701 gccgccagtt catcgtcgag cagggaacgg acaatgctgc tgcgccggaa aatcttgagc
    20761 ggcgacagca aaatcggcgg cgtcacgtcg ggaaagtagt agtgcacgaa gtagcctgcg
    20821 cacagccaca ggccaaaaat tccgatgagc accatcacaa agctcagcgt gccttgctgg
    20881 tacggcgagt agcggtattc aaactggccg cgatggatcg acggattttc atacacgtag
    20941 ccgcctgcgt cgtagtcttt gataaacgcg agcggaaaca gcgtgtcgtt tccgacggca
    21001 ctgacccaaa tggccgggcg gcttagcggc ttgtcgacgc cgggtgcggc ttgcgaatac
    21061 tcgacaagct gttcgggata gcccaaaagc gcgacggtgg cgttataaac cccttggtcg
    21121 gcatcgctcg caaactggat gcgctgacgc agcccgcgga acggatagct ccactgctgg
    21181 ttgcgggtaa aaagcgggta cggcgacacc accagcatcc ctttgagata cgagcgcgcc
    21241 tcgggatggg tatacaggat gtcgctttcg aacgtaaaca gcaccacgtc ggggctgtag
    21301 gcgcggatct gccgggcgag aaacagcttg tcctgtacgt cggtggcgag gattccgaca
    21361 tagcgaattt tttcggcgta aatcgtgctc aagatgtggg ccagcgagcg ttcggcagca
    21421 gacaccgtgc gcggcgacag cgtcgggatg atgtcggtac gcggactgtc atcgagcagc
    21481 gttggcaaag gccgactgac cggcgatggc acaagatcgg gcagcagcgg ttccgagctg
    21541 cgcggtgtcg acggcgaagt cttggccacg tccacggtgt gcagcctggc gatgtgcagc
    21601 ggcaccggca gcgtcagccg tggacgatgc gcgcgtttgc agtggttgct cttgtgcgtc
    21661 ttgtgggccg gaaacacccg atcgtagccc ggcggcagac cctgcgattg cgaagcctgc
    21721 gccgcaatca ccgcttgccc atatcccgtc gatgattccg aaatcagcgc cacatcatgt
    21781 tcatcggcgc ccagcgtttc ggtcagaaaa caaaagaact cggtctgcag cgcttcgtcg
    21841 ggaatcaccg tcgcctgata gcgaaccgag cgcatcccgt cgttccattt ttgaatttga
    21901 tcgcgaattg cgccgctcgt cgcccgtcca gaaagcatcc gaaaagacag cgacggaaaa
    21961 tgcgccaaga tttgatcgag cgaaaccacc gcgcccgaaa acgctgggcc gaccattcgc
    22021 acttcgttac aggactcgca gcgaccgtcg ggaaccagcg cgcgaatgcg cagcaaatcg
    22081 tcgatggcgg aaatgaacgc cggtttgtga atgccggcga tgggcgactc gccgacaagg
    22141 tagagcaaaa gcagaccacg acggcttccc ccatgcccac tcgccgtcgc agcgcgatgg
    22201 aacagggcta caccgggctg atcctgcgaa cagcttgact cgccactgcg cttgtcggcc
    22261 tgcgggttcc acggcagaag gtagcgatcg agcaaaaaac ccgtcgcctc cagtgcgcgg
    22321 gtaatcgact cgacggtgcc atcgaaaccg agccgaggtt cgggaaccgt cgcgaccaaa
    22381 aactgaacac tgacatcctg cgaaaccagc gcctgatgaa tcgcatcgtg gttcgcccgg
    22441 cctttgtcac cgtctggaat cgcaaaatat gccgacaaaa gccggctcgc ttccagcttg
    22501 ggacaggcgg ccccaggttc gttcgctggc ttggcttttt cagtcgaaac gggcggcaaa
    22561 accggctctg ctggcgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    22681 gacttgcgga cgccactgga cttgcggacg ccacggtggg atcgggaagc ggtcgcggcg
    22741 ggttcgccat cgacgctaca ccccaaagcc caagcagcag aagcacaacc gcacgcaggg
    22801 gatgtcgaat gcaaagcggt cttcgcatgg cttcgacgtt agcaaatccg gcggtctgct
    22861 tgggagcttg tgctcgcgat ttgtcgcgtc ttccgccgcg attagtagag atcgaagcgc
    22921 agcagccggc cttcaagcat gccgttccac aacacatgcc gcgcggacgg acgcagccca
    22981 atctgccgtg ccagctgtag gtttgccgtg tacacgtagg cgcgatagcc gacaaagcgc
    23041 cgcttgagcg tgtcaccaag cgcgcggtac agatcgacaa gctcgtgttc gtcgcccatc
    23101 cgctcgccat acggcggatt gcacagcacg atgcccggtt gtgccggcgg tggttcgaca
    23161 gagcgcagat cggcgcgaat cagccgtgac tggcgcccaa ggcccgccgc cgccaagttc
    23221 tgcccggcca tccgcaccgc ccgcggatca atatctgacc catacaggaa cacacccgcc
    23281 gctgctcgct gcttggcttt cgcttcctgc tctagcttgc gaaacaacag cggatcaaag
    23341 tcgcgccagc gcataaaacc aaagcgacgc gaaagtcccg gcgccacccg cagcaccgac
    23401 agacccgctt cggtaagcag cgtgccactg ccacagcaca gatcgacaat cgtcgacgga
    23461 cgcggctcgg ctgggcgtgg ctggtgcgcc ggtgtggtgt gcaccgcttc ggaaatcggc
    23521 gtcgcaccaa gcgctggttc gccggtttgc gccacaatcg ccgccgccag cgtttccttc
    23581 agcggagcaa gcccgccctg ttgtcgatag ccacgctgga acagcggcgg atcggacgca
    23641 tccgccccga tcatcatccg cgtgccgcgc gcccctgaat aaagctgcac gttgatccgc
    23701 agatccggct gctgggtgtc gatgtcaggc cgtcgaccga cgcgatcgcg aaagcgatcc
    23761 accaccgcat ctttggtctt tagcgccgtg aacatgctgt tggcaagacc gggcccttcg
    23821 cccatcgcgc tcacagccat cgttcccgac ggatcgatga attcttccca cggcagacgg
    23881 cgcaccgcgt cgtacagatc cgccggccct tcgcacggat aatccccaag cacttgcagg
    23941 attcgcgacg cacagcggga atacaagttg gcccgataca gcgccgcccg tggacccgaa
    24001 aaagacaccg cgccttggcg tacttccacg tcggtcacat cggggagcgc gaccagctca
    24061 gcagcgacca gtggttcggt gttgcgggcg caaagcgcca gatagcgagg ccgactcgca
    24121 gcgaccggtg ccggtctggc gatatgggct cctgtcatcg tcggttctcc taggcgcggg
    24181 gttttcgcga ctttagtgac ccaggcccct gcaagtcaac gcgaccttgc gcagttgact
    24241 tcccaagcca tctgcctgca ccgtaagcgc catgagttcc gactgcattt tctgcaagat
    24301 tgcaagccgc gccatcccgt cgcccttggt ctacgaagac agcgaagtgg tcgcgttcca
    24361 agacatcaat ccgcaggccg cgcaccacgt ccttatcatc ccgcgtcagc actttgccga
    24421 tatcggggaa gcggcggcgg cgggacaaag cgccctgttg ggtcagcttg tcacggcggc
    24481 ggcgcacatc gccaaagagc gcggattgtc cccaagcggc tatcgcttgg tctgcaacac
    24541 cggcaaagac ggcggtcaga cggtgcgtca cctgcacttc cacttgcttg gtggacgacc
    24601 actgggctgg ccaccgggct agcgaactgg cccgtggcgc ggcgggttcc cgcgcgg
//
LOCUS       DAORGN010000055        24572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_802_length_24572_cov_5.146143, whole genome shotgun sequence.
ACCESSION   DAORGN010000055 DAORGN010000000
VERSION     DAORGN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 24572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24572
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_802_length_24572_cov_5.146143"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1793
                     /locus_tag="PKL17_08350"
     CDS             171..1793
                     /locus_tag="PKL17_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNK44777.1"
                     /translation="MHPLPIDPRIVEECRSLAGQVADQVQSFISAHTTVSIERTVLRA
                     YGVDGVDGEGVPLVNRCVDRYRGSAALGHGIAALLGLLLVRRPGLPIQEAAEELAFGH
                     TLPEGADLLSALDAERAAIEAVLVEPTRLAIEKIDVAHSARIAAWQRLGLGPDRAPAT
                     GNVIRPGGGPPLKYVIVATGNIYDDADQARAAAQVGADVIAVIRSTAQSLIDYVPYGA
                     TTEGYGGTWATQENFRIVRRALDEEQEKLGRYLQQVNYSSGLCMPEIAYLGAVERLDM
                     LLNDAMYGILFRDINPKRTLCDQYFSRRIIGRAGIIINTGEDNYLTTADAVEKAHTVL
                     ASQFINEAFAFRAGLCEEQMGLGHAFEIDKALPDSFVLELAQALLVRQLFPKHPIKWM
                     PATKHKTGDIFFSHRVDGLFDLLGVMTGQTIELLGMMTEAIHTPLLHDRWAAIKGADY
                     VFTAARSLGKELRFDPEGQVVARAKQVLDEAVTLLRKVAQEGLFAAIAAGEFADVRRP
                     ENGGRGLDGVIARHPGYTNPILRILEGHNPTG"
     gene            1976..2725
                     /locus_tag="PKL17_08355"
     CDS             1976..2725
                     /locus_tag="PKL17_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNK44778.1"
                     /translation="MGEYPGLDIKTLSEGDRGLLKRLLEKFPSACGKPHSLAVSLRTD
                     PSCKLSGIASKWLLKMVIDGFLESEIEEKYSERYLQRKCYSVDIAQAPVRGDAKAPVT
                     IIEFSDFECPACRAVEPMLKQVLAEYSKVRLVFMNYPLPMHPNAMNSAMASVAAGKQG
                     KFWSYHDKLFENQDKQTMADLVRYAMEMKLDVTKFQADMQAARERVEKDRAEGKKVEL
                     TGTPGMLVNGCKVRAGSIEELRSYIDAELAK"
     gene            2722..3231
                     /locus_tag="PKL17_08360"
     CDS             2722..3231
                     /locus_tag="PKL17_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNK44779.1"
                     /translation="MTGLRLAIATLCLSVSLGVAAAGQQAGKPAADFSLPDAAQKTVR
                     LSDLRGKVVLLDFWASWCEPCLRELPELEKLHKQLASRGVVVIGVNIDRDRKNADELI
                     SRYKLSFANLFDPKGQIVELYDPPKMPTSYVIDAQGNVRLVNEGFAGPADIAKLRTAI
                     DQILANPAK"
     gene            complement(3250..4035)
                     /locus_tag="PKL17_08365"
     CDS             complement(3250..4035)
                     /locus_tag="PKL17_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNK44780.1"
                     /translation="MQAEPSRFAPPCRLVDLTFPEAMTLRAAQPVVLLPMGSVEAHGP
                     HLPLGTDSFLSESLALSAQKQLHQTQTLSVIAPTLSYALTHFASEFAGTVSLSAAAAT
                     GQLRGVVEAFLAAGFSRICLVNSHLEPAHIQGIRTLVSDLCTQHGPVVAFPDQTAKRW
                     ARTLSAEYKSGSCHAGSYETSLLLVARPDLCRNEIAQQLPNNPSDFLAKVRAGAQTFR
                     EAGGDSAYFGRPAESSQQEGEAQLALLTQMVVTTIAETWPKSS"
     gene            complement(4038..5318)
                     /locus_tag="PKL17_08370"
     CDS             complement(4038..5318)
                     /locus_tag="PKL17_08370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNK44781.1"
                     /translation="MSSYGPSDHSEDNTPRPRRKTTTFVAPAPRLRRGSVRLPFDAAR
                     RLAAGHPWVFRDTIGRPLSSESGDVIELFDPEGNFIARGIYDAEGPIAVRAFTRDPDE
                     VLDEAALLRRVRAAAKLRKTLLPTDELTAYRLIHGEGDFLPGMTVDRYGDHLVVHVFS
                     ASLAKLTDQLVPVLREVHAPRSIYVQKRYRPLGGEAPREPAELAWGEVAPVEIEVREG
                     KMRFGVDVTAPLGTGLFPDLRNGRNAVTARAQGRRVLNLFSYTGALSLAAAVGGAKEV
                     VSVDIAAKAHARARRNLQLSGLSEDIHEFIAADALTVMARMADRKRQFDMVILDPPAF
                     AHSRERSFSVEKDYRELVSAALRICDSGALLCCAVNAHRFSLESLLLELGEGASRSRR
                     LVRVVEQLGLPADFPLPAGFPDGNYLKFVICSVV"
     gene            5505..7055
                     /locus_tag="PKL17_08375"
     CDS             5505..7055
                     /locus_tag="PKL17_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44782.1"
                     /translation="MSTQNQGKQPRSVPPPPPKVASHSRPNLRRQSMDDLLDDFIVRA
                     NQRVSSIRSRQWDLLVPAEEVDVSQIISDEPPVAVHAAPAAQVATVIVAEQAAPAPVV
                     VPVPEPVLASVPAPVASVEPTVAQVEESPAAPATAQAIEVVAAQAVPSAPEVVAPAVE
                     PPATVVDAAVDAAPAVEAKVEQATAPEPSGATTQPAGHKGNKKKNRDRQRQQSQAMPG
                     RDDRQEPVSESAPAATTTEPEPTPEKVEAAKPAAVVAAPAKAPVRSEAAPVKSESRPR
                     VAPVPLEPPPKSGTSKGTLVIGMAVAAGLAVGAMYYLQNGSQNNVTPPPAAVTPPPAA
                     VTPPPATVTPPPAAAVPPPVETTAGQPAATAPATAAPTAAVAAPEVEKAKPAAVAPEA
                     TATSSKPATEAAKAEPAPVKAEKAAAAEAPAKSEPVAKPEKAAKAEKPAKAEKPAKAE
                     APAKAETPAKSEPAAKPEKAAKAEKPAKAEAPAKSEPAAKPEKADKPDKAPAKSKKDE
                     SWIDPFAQ"
     gene            7086..8711
                     /locus_tag="PKL17_08380"
     CDS             7086..8711
                     /locus_tag="PKL17_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44783.1"
                     /translation="MRKKTLLTATAVALVAMTIPVESQWLCGVGLRSASAQEEDPEIT
                     AEAKKHYKLGQDAFTEGKYDLAIKELKRAYVLKRIPAILVNIAMTYRKTKDYEMAIYF
                     YKKYLTDSPADDKQRPAIEAAIGEVEKERSAAAQPQLAPAKPAVEMAKPAEAVKPAVE
                     AVKQAEPGKPAVEAAKPAVEAAKSAEAVKPATEAAKPAEAVQAPAPAAPEKSATEWAH
                     TPVDAAPPGQPVDIRVQMPVMKGVKVKVYFRKEGQATFDSLELKRRGNEKVARIPESV
                     AQGKAFQYYIEAKDAAGTIVKSIGNDMSPNLVLIDPTARPQIIGAEVQGETADDDEVA
                     KRTKTGPTRDIENEQATFDLGGQSAAMARLRQQMRDREKSKSKGSGKSLLGPMGWGGG
                     AVALLGLGGLGAGGAFLGMASNYANLVSSDSACDTKVKVDGVPQCPHFGPNDNPDLSK
                     TLKPATAEYERKGMLYDKVGTAVTAVGGVLLAGGAAMLTYDIIKRYGKEKPPAPKTRK
                     VKKVIEVEEPATSWLIAPVVSPGSVGIVSLFNY"
     gene            8733..9635
                     /locus_tag="PKL17_08385"
     CDS             8733..9635
                     /locus_tag="PKL17_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44784.1"
                     /translation="MRLSKLLTLGLLLSAACYKPSYSSDEDLGKAFRCYVNDKPACPG
                     DLVCCVGDECGDALLDPITGMPRIDNGTGRPLEGSCVKPRPPKDMVVTPVGYWDFGIK
                     GSYYTGIQNDPMLTGKDPDTGKWRCKRDDLNMSKPAEIERMFEPNDLPDQAISLSNPL
                     PIDPTPGPGTSYEICPDQSSPDLPDVDVFKFRLTSPTKVIVRLTYKVSYGDLDVALFR
                     LAKDDATGELKPQRVLADLTAVDNGCLEAPNLQPLGPREYYYIVVRGTNSPDKPSSYS
                     MNNYSIRVYGTTTDSCVAKKDGGV"
     gene            9710..11692
                     /locus_tag="PKL17_08390"
     CDS             9710..11692
                     /locus_tag="PKL17_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNK44785.1"
                     /translation="MQTSPRRAMTLTQKILAAHAIGLSRPWVKAGDILRVRVDWTIAS
                     ELAWNGMDKTYEALGRPPLHSPDRFFLAVDHTVDPTTLRSDVRAQKLTQLSRSFAKER
                     KLRHFYDANQTILHTKFYRDLLLPGQVVIGADSHTSSHGGLGAFAIGLGGADVTAAMV
                     LGESWIEVPEAIAVDYVGTPAFGMTGKDIILRTLGDLGRNTVAMERSVEYRGEAVRNF
                     STDMRFTIANMTAEFGGFSGIFEPDAVTAAWLAQRSEPPKAGEALFFRADDDAEYRTR
                     HTIRLDSLEPLVAKPFSPDNVVPLSQHQGMPIHGAFIGACTTTEEELVLGALVLEEAL
                     RRGIVPTGSGRRLVVPGDLSIQGRLYEAGLLRVYEQAGFQVDPPGCSMCLGVASQRAG
                     KGETWLSSQNRNYPNRMGEGSLAWLASGPAVAAAAIQMQVRDPRELLAGIDPDRYQRI
                     LRRSGHSLPTVTTSEPTVSISAGTGSAAHATQTAAAQTVQGRVQRFGNHVDTDAIIPG
                     QFCHLTDFSELGDHCFEFVRPGFASAVRAGQNIVVAEEGWGSGSSREQAVWALKGAGV
                     SVVIAKSYAFIHKRNLVNEALPFLVVTDEEFYSLCADNDELSVDLSGTVRHVASGRTW
                     SAQPTTALVSALVREGGLVPAIKHHGQTVFATLTTG"
     gene            complement(11999..13390)
                     /locus_tag="PKL17_08395"
     CDS             complement(11999..13390)
                     /locus_tag="PKL17_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44786.1"
                     /translation="MPVLATLLLTLFAAFVLWRLLRSFRHGLSIRMQVFVAMATVSGG
                     FAALLGLLAVQRLELRAARLLAEVSREEAAILSSVISLTGKTPQDLTDVPSLQKLAEV
                     RPGGLHIEVVDPEGRVLLASGAPIPQRPIEQALLRSPQGAVLGAVRVSRESLGFRQIL
                     REVASRAALLTLLLVLGTASAAALIGRAIASPIEQLTQAASRIAQGERQAVLPPPFGR
                     EVRTLTHAVESMRRELEGRHLAEKLAADLSHELKNPVAAIRAAAEVLADGALDEPDTA
                     RRFVARIAEAAQRLQGILGNLLMLTRLQARGVVREPVDLAELTRQSIDAHAAQATQRR
                     IEVRAELPGELMVPGDEPWLRRAVDNLVDNAIAFADVSHGVAAEPAVTVRLRHDEERA
                     ILEVENRGAGILPDIRGRLFERFVTTRRDTGGSGLGLAIVAAVAEQHGGRVEVSDAGP
                     PKTQLRLTLPALD"
     gene            13448..14152
                     /locus_tag="PKL17_08400"
     CDS             13448..14152
                     /locus_tag="PKL17_08400"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44787.1"
                     /translation="MKTKTQLGLALVLSCLPLLAFADKAPPPHAHHGGPGEKPMVHRF
                     EDPAKWTAVFDSPDRDGWQKPQEIVKRLQLSPGSTVVDIGAGTGYLMPHLAAAVGDKG
                     KVIAVDIEPKMVDYLTERAKKAKLTQVTAQLGLPTDPKLAPASVDKIVFLDTWHHVPS
                     RIEYAQKLFAALKPGGTVYVVDFTMESSHGPPKKHRLLPEAVSLELSTAGFVAEVLPE
                     DLPDQYIVAGKKKANP"
     gene            14177..15184
                     /locus_tag="PKL17_08405"
     CDS             14177..15184
                     /locus_tag="PKL17_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNK44788.1"
                     /translation="MLAKRLLSLGLTMGFSTLLACDPVPPAETDTAPLSSEQALHAGS
                     AIQQVFVVMMENQNAKDVYGNPDAPYINQLMKDYGYASNFQDCLPSSVPSEPHYIWLE
                     AGTNAFSDVTFGGDGDATPRNSTASTAHLVNLLDKAGISWAAYQEGIDSTISGACPIS
                     SNGLTRYAAKHNPFVFFKDVSGNPPSKTASRCVAHNKPLSQFASDLSTGSVARYNLIT
                     PNLCHDMHGALGCSGGSNVKQGDTWLKGFLPSLITYAKANRGVILLTWDETEGTTTQP
                     FLVIGSGLKRSGYESKVRYDMSSVLKSIQRIFAVTPLLGHAADASTTDLSDFFATGSF
                     P"
     gene            15213..15935
                     /locus_tag="PKL17_08410"
     CDS             15213..15935
                     /locus_tag="PKL17_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK44789.1"
                     /translation="MSLFEPSLTVVIPAFNEADNLKVVLPDALRFMSERCVNCTLLLI
                     DDGSKDGTASVIAEYVANHPQRVQVITHPRNLGLTAALRSGFFGAHSELVTWIPADGQ
                     IPISELDKMLRAYRGEDLLLTTYRHRPDGLWRATMSKTVRIMVRLATGFADRLEGPYL
                     FRRALIDELNLVCMRSAGSIGLEIAAKSHALGKRIGSTEIECVPRLSGRSKVADLRNI
                     VTYFGEIWLIHRSQSELLASRR"
     gene            complement(15831..16775)
                     /locus_tag="PKL17_08415"
     CDS             complement(15831..16775)
                     /locus_tag="PKL17_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK44790.1"
                     /translation="MKRALSALRVVPRAAKLRVLALLCGAALLIGLPWLAKPSHPGLL
                     LLGELLFLSALLGFLWCRIPCGLWLVPRDLHGFVDGNSDEQVAFSFDDGPTAGLTDAL
                     LDLLLAHGVRASFFVLLHKAETHPQIIRRIVREGHVLGLHGEDHRLPLWQTHTQLTQS
                     LLRAKERLMAIAGQPITLFRPSHGVRTPALLSALRTSDLRLCMWDHGVWDTDAPPPAT
                     LLSRLHIVQSLGRKADRPRVLLLHDGLGDEPGIPPHAQPLLYAVQQFLRDTKTQSDRT
                     QPGVLATAGEQLGLRAVDQPNLAKVRHDVAQVGDLGPP"
     gene            17053..19599
                     /locus_tag="PKL17_08420"
     CDS             17053..19599
                     /locus_tag="PKL17_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK44791.1"
                     /translation="MDRSTSRQGGSLLSWFAIFHRRYRSVLTQAMLAGTAASGLVYLD
                     DTLLGWITSSLADPGQGKEMPVPMLVGDLANRLGIGLPFLLVAFFFVNRLVTAGIGFW
                     QIYCTGVLNLRGKADLEREILTHLLRKDDAFFGRHSPAETVNRLSIDLQRICARRPNL
                     MMFWWSALLIIGNLTYFVVHDLRLSLVALASCVLGGFWASTMSGRVKQTDARYLHEDD
                     QVKRCFEDYLRAFQEVQVGKLQDKVTAHLGQLQEHRGQTFRRYVIINESVAVGNLMAN
                     LLAFVAMLLVVIYMRKSGAVSRALMLIPVIIHTLPYLIKHMVQLINLSMDYQLSRTSV
                     HRLLEYETDSDEESGGASAKDPIPTTVKLHNVTYQYAGPDGSRQGGVVAVQAELLPGK
                     LTTIVGGAGSGKSTFIKLLLGRLVPQDGAVQYGDATLATLQKRELAAMCTLMPQTLAL
                     LDGSIAQNILFGRPRGQDTLDAQDFDVVEKSGLGRICRLKALEMAPDPKIEVPELYRD
                     IAALRSQLRQRLLEKCGVTVRGYEEGHADPKHWALEMLLGGRCDRARTLSLLLADSTR
                     RSLAALLSTEEGKLLIDRACHVLAESSGLLSLDNFHVYSQLSPWPLEEKVWQLRRSFL
                     HLSARRPDGKRELLAALTIGLTSSLGENAASTEQLKTVRRSEQAAGSLRLLRDLLGSV
                     CRPFQLDSVHPFLSWRENLTFGVIDLPNSRAGRLVEQTLLQFQEQDGLREAFTRLGVQ
                     FGIGRQGANLSGGQGQLVALVRALLRKTQVIVLDEPTSALDPASRARVVELLTDWKKD
                     RVVVTISHDPEFARQADHVLLMDGGRLIASGTFDELKETNEVFRRTLRQT"
     gene            19596..20036
                     /locus_tag="PKL17_08425"
     CDS             19596..20036
                     /locus_tag="PKL17_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNK44792.1"
                     /translation="MNAMPLSAAEKMNVLTRCVVFAGIPSRDLSVLAEMMTTERLSAQ
                     EVLFEKGDPSDSVFVVADGKLGIFLDPAGKPVRILGPGELLGEYGMFIGLFRTATVRA
                     DSSAVLLSLDYQRFRAFLLQFPQSALVLMKIAVQRLVAAESARR"
     gene            20196..21056
                     /locus_tag="PKL17_08430"
     CDS             20196..21056
                     /locus_tag="PKL17_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK44793.1"
                     /translation="MNDLDAWLELDDEKIPLADDCSIGRTQSNTLPLRDDKVSRAHAR
                     IYRQEDEFCVVDLGSANGTFVNGRRVVQPVQLRSGDVVAVGPFELTFRRNEKAASIPP
                     PSLDESLNVQTLVHVRSVDCYLLVADIIGSTQLVTTHSSDALPFITGRWFTRCQEVIE
                     RNKGSINQYLGDGLFAYWQSNNVSPMQVAEGLRQLAYLQATEETRFRFVVHFGPVSTG
                     GVVATGEAPLFGQDVHYVFRMEKLAPQLNLTRMMSHSAKELLGRFLPTSEAGSHPLSG
                     MKGTHRMYSF"
     gene            complement(21117..22064)
                     /locus_tag="PKL17_08435"
     CDS             complement(21117..22064)
                     /locus_tag="PKL17_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK44794.1"
                     /translation="MKWLDVVVAPKHKASDLPHSALSEGEAPSIRFDIWGCRGSRNIL
                     PAMSKIGNCTSCYSLSHGDVLFALDAGRGLTALSTRLFGRGRLAQIRHVVVLVSHSHL
                     DHWEGIKDAEWFWRRDNGISLSICGPKEALQAIEQGLRHPSFVPLQILSLGTLADLEF
                     RTLHEGQRLTLFGWQVEPFALHHYSGSGAQRQMLDALGYRVTVPSGPTVTYLCDHEPT
                     QATRAAEERALLGSHLALYDAHFPHIKEQTFGHGSIEHAGIMAREHPDKLILAGHINP
                     RYSDAEVRRFLTQHGRGTPNLRIAIENQSYAWDGNRFHR"
     gene            22225..22971
                     /locus_tag="PKL17_08440"
     CDS             22225..22971
                     /locus_tag="PKL17_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK44795.1"
                     /translation="MRLSDMKVIVTGGAQGMGAHFVKRLHEAGAQVAAGDVNEAGLAE
                     LPAGVHRRRLNVADEADVQAFVAWAHGAMGGLNGLINNAGILRDGLLVKKDKATGAVI
                     KMSTDQWNAVLAVNLHGATFMVREVVAKMAETDSRPGVIVNMSSLARYGNRGQSNYVS
                     AKAALAANAVTWAREFGPLGIRCVAIAPGMIETPMTQGMNQKARDALVAAVPVGRVGV
                     PEDIWQAVKFAIECDYFNGRCVDVDGGLVM"
     gene            complement(22984..>24572)
                     /locus_tag="PKL17_08445"
     CDS             complement(22984..>24572)
                     /locus_tag="PKL17_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNK44796.1"
                     /translation="DRGNVAILVAMLDRSEEQRAAELLKAILSSVSDAVITTDEDGSI
                     VMANAATERVLGYAPSELTGQNIKQLIPVAQHASHTAAMNRWREQNGYLLGDNRREMT
                     PQRKDGTTFPAEITVASFQINGKPHFTGVVRDLTVRRKLEDQLRQAQKMEAVGQLAGG
                     IAHDFNNLLTVINGQTSRLLHKLPHDDVLHDPLTDILAAGERAATLTAQLLAFSRRAI
                     VEPRLLDLNQVVMGTTRMLRRLIGEDITLLTQLRPGPTTVKMDAGQLEQVLINLALNA
                     RDAMPKGGKLTIGTAQQDITAPSSDRDREPAPGSYILLWVADTGIGITDAVKSRIFEP
                     FFTTKGVGKGSGLGLATVYGIVRQAGGYISFESEAGKGTTFRVLLPQQKKLPVSAHAA
                     SRLDSPTGTETILLAEDEDVVRKLVRQVLELHGYTVIEASGGQHALEVAREYEKPIDL
                     LLTDVVMPGLGGRELADEVRRHRPDIRVLYMSGYTDDAITRHGVAAAVDAFLQKPFAP
                     PQLLRKIRELLNQKSHSDRS"
BASE COUNT         4878 a         7440 c         7642 g         4612 t
ORIGIN      
        1 agtctgagcc atagaagtat ctgcctccgt gtagggcgtg gccacttggg cgcgagaagc
       61 tccgctaagt caggaaaatc cgctctccat catatcgcaa tcgggcgctt tcttgctcag
      121 acccccgccg tgaagttgat ccacgtttcg cgaggacata cgctgcggtc atgcacccgc
      181 tgccgattga ccctcgtatc gtcgaagaat gccgcagcct ggccggtcaa gttgcagatc
      241 aggtccagtc atttatttct gcgcacacca cggtcagcat cgaacggacg gtgttgcgcg
      301 cctacggtgt cgatggtgtc gatggggaag gcgttccgct cgtcaaccgc tgcgtcgatc
      361 gataccgtgg ctcggcggcg ctggggcacg gcattgctgc gctgctcggg ctgttgcttg
      421 tgcgacgacc ggggctgccc atccaagaag cggcagagga actggcgttt ggccacacgc
      481 tgcccgaagg cgcggatctg ttgtcggcgc tcgatgcaga gcgggccgcg attgaagcgg
      541 tgcttgtgga accgacccgg ctcgcgatcg aaaaaattga tgtggcccac tcggcgcgga
      601 ttgccgcgtg gcagcggctg gggcttggac ctgatcgagc acctgcgacg gggaatgtga
      661 ttcgtcccgg tggtggaccg ccgctgaaat acgtgatcgt ggcgaccggc aatatttatg
      721 acgatgccga tcaagcgcgt gccgcagcgc aagtgggcgc cgatgtgatc gcagtgattc
      781 gatcgacggc gcagtcgctt atcgactatg tgccgtatgg agcgacgacg gaaggctacg
      841 gcggaacctg ggcgacgcag gaaaactttc gcatcgttcg tcgcgcgctc gacgaagaac
      901 aggaaaagct cgggcgctat ctgcagcagg tgaactattc gtcgggactt tgcatgcccg
      961 aaattgccta ccttggcgcg gtggagcggc tcgacatgct gctcaacgac gcgatgtacg
     1021 gcatcttgtt tcgcgacatc aatcccaagc ggacgctctg cgaccagtat ttttccaggc
     1081 ggatcattgg ccgcgccggg atcatcatca acaccggcga ggacaactat ctgacgacgg
     1141 cggacgcggt cgaaaaagcg cacaccgtat tggcgtcgca gttcatcaac gaagccttcg
     1201 cgttccgggc tgggctttgc gaagaacaga tgggcctggg acacgctttc gagatcgaca
     1261 aagcactgcc cgattcgttt gtcttggaac ttgcgcaggc gcttttggtt cgacagctct
     1321 ttccaaaaca cccgatcaag tggatgccag cgacgaaaca caagacgggc gacatcttct
     1381 tttcgcatcg ggtcgacggg ctgttcgatc tgctcggggt gatgaccggg caaaccatcg
     1441 agctgctcgg gatgatgacc gaagcgattc atacgccgct gttgcacgat cgctgggcgg
     1501 cgataaaagg cgccgactat gtgtttacgg cggcccgcag cctcggaaaa gagctacgtt
     1561 tcgatcccga aggccaggtg gtggcgcggg ccaaacaggt gctcgatgaa gcggtgacgc
     1621 tcttgcgcaa ggtggctcag gaaggcctgt ttgcggcgat cgctgccggt gagtttgccg
     1681 atgtccgtcg tccagaaaat ggtgggcgcg ggctcgatgg ggtcatcgct cggcatccgg
     1741 gctacacaaa cccgattttg cgcatcttgg aaggacacaa ccccacgggt tgagatcgta
     1801 tttcgtgata gaaagggacg ttcagccgtc caagactgca tacaccatga cgcacagcgc
     1861 ttctttcctt cgctctaccg cttctgcggt cactttcggc ctcggcctgt tcctttcctc
     1921 ggcggtcttt gctgtcgggg aagcacccaa gccagcggcg ccagctccgg cggctgtggg
     1981 cgaatacccc ggacttgaca taaagacgct gtccgaaggc gatcggggcc tgctgaagcg
     2041 gctgctcgaa aagtttccgt cggcgtgtgg caagccgcat tcgctcgcgg tgtcgctgcg
     2101 gaccgatcca agctgcaagc tgtcggggat tgccagcaag tggctgctca agatggtgat
     2161 cgacggcttc cttgagagtg aaatcgagga aaagtacagc gagcgctacc ttcagcggaa
     2221 atgctacagc gtcgacatcg cgcaggcgcc ggtgcggggc gatgccaaag cacccgtgac
     2281 gatcatcgag ttttccgatt tcgagtgtcc cgcctgccgc gccgtcgagc cgatgctcaa
     2341 gcaagtgctg gcggaataca gcaaagtgcg cctggtgttt atgaactatc cactgccgat
     2401 gcatccgaac gcgatgaatt cggccatggc ttccgtcgct gctggcaagc aaggcaagtt
     2461 ctggagctac cacgacaagc tgtttgaaaa ccaggacaag cagacgatgg cggatctggt
     2521 tcgctacgcg atggagatga agctcgacgt gaccaagttc caggccgata tgcaggcggc
     2581 gcgtgagcga gtcgaaaaag atcgcgccga aggcaaaaaa gtcgagctga ccggaacgcc
     2641 tggcatgctg gtgaacggct gcaaagtgcg cgcgggatct atcgaggagc ttcgatccta
     2701 catcgatgcg gagctggcca aatgaccggg ctgcggctgg cgattgcgac gctgtgcctt
     2761 tcggtttcgc ttggtgttgc agcggcggga cagcaagcgg gcaagccggc tgcggacttt
     2821 tcgcttcccg acgcggcgca gaaaacggtt cgtttaagcg atctgcgcgg caaggtggtg
     2881 ctgctcgatt tttgggcgtc ttggtgtgaa ccctgcctgc gcgagctgcc cgagctggaa
     2941 aagctccaca aacagcttgc cagccgcggc gtcgtcgtca ttggcgtcaa catcgatcgc
     3001 gatcgcaaaa acgccgacga gcttatcagt cgctacaagc tgtcgtttgc caacttgttc
     3061 gatccaaaag gccagatcgt tgaactatac gatccgccga aaatgccgac ctcgtatgtg
     3121 atcgatgcgc agggcaacgt ccggctggta aatgaaggct ttgccggtcc cgccgacatc
     3181 gccaagctcc ggaccgcgat cgatcaaatc ctcgcgaatc cagccaagta gcgacgacag
     3241 ccggtcttcc tacgaagact tgggccacgt ctcggcgatc gtcgtcacca ccatttgggt
     3301 caacagcgca agctgggctt cgccttcttg ctgggacgac tcggcagggc gaccgaaata
     3361 cgcgctgtcg ccacccgcct cgcggaaggt ctgcgcaccg gcccggactt tggccaaaaa
     3421 atcgctcgga ttgtttggca gctgctgggc gatttcgttt cgacacagat cggggcgagc
     3481 gaccagcagc agcgacgttt cgtaactgcc agcgtgacag cttccgctct tgtattccgc
     3541 cgacagtgtg cgcgcccagc gcttggccgt ctgatcggga aatgcgacga ctgggccgtg
     3601 ctgcgtacac agatcggaca ccagcgtgcg aatgccctga atgtgggcgg gctctaggtg
     3661 gctgttgacc aagcagatcc gcgaaaaacc cgccgccaaa aacgcttcga caactccgcg
     3721 tagctgcccc gttgccgctg ctgcggaaag cgacaccgtt cccgcaaatt ccgacgcaaa
     3781 gtgggtcagc gcgtagctta gcgtcggggc aatcaccgac agcgtctgtg tttggtgtag
     3841 ctgcttctgc gccgacagcg ccagggattc cgacaaaaac gaatcggtcc cgagcggcag
     3901 gtgcggcccg tgtgcttcga ccgatcccat cggcaacagc acgaccggct gggccgcgcg
     3961 cagcgtcatg gcttccggaa acgtcagatc gaccagacga caaggcggcg caaaccgcga
     4021 cggctcagcc tgcactgcta caccacgcta cagatcacga atttgaggta gtttccgtcg
     4081 gggaatcccg caggaagggg gaagtctgcc ggcagtccga gctgctcgac gacgcgaacc
     4141 aagcgccgcg atcgactcgc gccttcgccc agttccaaaa gcagtgattc gagcgaaaag
     4201 cggtgcgcat tgaccgcgca gcacaagagc gcgccgctgt cacagatccg cagcgccgcc
     4261 gataccagct cgcggtagtc cttttcgaca gaaaacgaac gctcgcgtga gtgcgcaaac
     4321 gccggtgggt cgaggatcac catgtcaaac tgccgcttgc gatcggccat ccgcgccatc
     4381 accgtcagtg catctgcggc gataaactcg tggatgtctt cgctgagccc ggaaagctgc
     4441 aagttgcgac gggcccgcgc gtgggctttg gccgcaatgt ccaccgatac gacttctttg
     4501 gcaccaccga cggcggctgc cagcgaaaga gcacccgtgt agctaaacag gttgagcacc
     4561 cgtcgaccct gcgcgcgtgc cgtgacggca ttgcgaccgt ttcgcagatc gggaaacagt
     4621 ccggtgccca gcggcgcggt gacatcgacg ccaaagcgca tcttgccttc gcgcacttca
     4681 atctcgaccg gcgcgacttc gccccatgcc agctcagcgg gttcacgcgg ggcctcgcca
     4741 ccgagcggac gatagcgctt ctgcacataa atcgatcgcg gcgcgtgaac ttcccgcagc
     4801 actgggacaa gttgatcggt cagcttggcg agcgacgccg aaaagacgtg gacgaccaga
     4861 tgatcgccgt atcgatcgac ggtcatgccg ggcaggaagt cgccttcacc atgaatcagt
     4921 cgataggcgg tcagctcatc cgtcggcaaa agcgtcttgc gcagcttggc cgcggcgcgg
     4981 acccggcgca ggagtgccgc ttcgtccaat acttcgtcgg gatcgcgggt aaacgcccgc
     5041 accgcaattg gaccttcagc gtcgtagatg ccgcgcgcga tgaagttgcc ttcgggatcg
     5101 aagagttcga tcacgtcgcc cgattcgctc gaaagaggcc ggccaatggt gtcgcgaaat
     5161 acccagggat gaccggcggc cagacgtcgt gcggcgtcaa atggcagacg gacgcttccc
     5221 ctgcggagac gcggtgccgg cgcaacaaac gtggtggttt tgcgcctggg ccgcggagtg
     5281 ttgtcctctg aatggtcgct tggaccgtag cttgacatgg cgcgcagcat acgggccaag
     5341 cccggtacag gcaagagggg taagcggctg tgtgtgcggg tgcggtcgcg cgtacatttg
     5401 gcttgaaatg gctacgcggc gaaaggtaaa acctccggct agcgtccctc gtcccttggg
     5461 ggtcgtgtcc gccaaatggg cgggaactgg aaggcgcagc caagatgtcc acgcagaacc
     5521 aaggcaaaca accccgttcg gtacctcctc cgcccccgaa ggtggcttcg cacagccgtc
     5581 ccaatctgcg gcggcaaagc atggatgatc tgctcgacga ctttatcgtt cgggccaacc
     5641 agcgcgtcag ttcgatccgc agccggcagt gggatcttct cgtcccagct gaagaagtcg
     5701 acgtctcgca gatcatctcg gatgaaccac cggtcgcggt ccatgcggca cccgccgcgc
     5761 aagtcgccac cgtgatcgtc gcagagcaag cggcgccagc gcccgtcgtg gtgccggttc
     5821 cagagcccgt tttggcgtct gtgccggctc ctgtggcaag cgtggaaccg acggtggccc
     5881 aggtggaaga atcccctgct gctcctgcga ccgcccaggc gatcgaagtc gtggcggcac
     5941 aggctgttcc ttcggcccca gaagtggttg ctcccgctgt cgagccacct gcgactgtgg
     6001 tcgacgctgc ggtcgatgcc gcgccagcag tcgaagcaaa agtggaacaa gcgacggctc
     6061 cggaaccgag cggcgcgacg acccagcccg caggacacaa gggcaacaag aaaaagaatc
     6121 gggatcgtca gcgtcagcag tcgcaagcga tgccaggtcg cgacgatcga caagagccgg
     6181 tgtcggaatc ggcacctgca gcgactacga ccgaaccgga accgacccca gaaaaggtcg
     6241 aagccgccaa gcccgccgcg gtggtggcag cgcctgccaa agcgccggtc cgttcagaag
     6301 cagcgcccgt caaatcggaa tcgcgtcctc gcgtggcgcc cgttccgctt gagcctccgc
     6361 cgaagtcggg cacgagcaaa ggaacgctcg ttattggcat ggcggtcgcg gcgggtcttg
     6421 ccgtgggcgc aatgtactat ctccaaaacg gctcacagaa caatgtgacg cccccgcctg
     6481 ctgcagtgac gcccccgcct gccgcagtga cgcccccgcc cgccacagtg acgcccccgc
     6541 cggctgctgc ggtgccacca cctgtggaaa cgacggctgg gcagccagcg gcgacggctc
     6601 cggccacggc tgctccgacg gcagcggtcg ccgcgccaga agtcgaaaaa gccaagccag
     6661 cggcggttgc cccggaagcg acggcgacga gcagcaagcc ggcgacggaa gcggccaagg
     6721 ctgagcctgc accagtgaaa gccgaaaagg cggctgcagc ggaagcaccg gcaaagagcg
     6781 aacccgtcgc gaaaccggaa aaagcggcca aggcagaaaa accagccaag gcggaaaaac
     6841 cagcgaaggc agaagctcca gcgaaggcag aaactcctgc caagagcgag cccgccgcga
     6901 agcccgaaaa agcagccaaa gcggaaaagc cagcgaaggc ggaagcccca gccaagagcg
     6961 aacccgccgc gaagcctgaa aaggccgaca agcccgacaa agccccagcc aagtccaaga
     7021 aggacgaaag ctggatcgac ccgtttgcac agtaacgagc tattgggaag cccgaaggga
     7081 gcaagatgcg caagaaaact cttctcacag ccaccgcggt tgccctggtt gcgatgacca
     7141 ttcccgtcga gagccagtgg ctctgtgggg ttggtctccg ctcggcctcg gcgcaggaag
     7201 aagatcctga gatcaccgca gaggccaaga agcactacaa gctcggccag gacgccttta
     7261 ccgaaggcaa atacgacctg gcgatcaaag aactcaagcg cgcctatgtg ctcaagcgca
     7321 tcccggccat cttggtcaac atcgcgatga cctatcgcaa gaccaaggac tacgagatgg
     7381 ccatctactt ctataagaag tacttgaccg actcaccggc ggacgacaag cagcgtccag
     7441 cgattgaggc cgcgattggc gaagtggaaa aagagcgcag cgccgcggcc caaccgcagc
     7501 tcgcgccagc caagcccgcc gtcgagatgg ccaagcctgc cgaagcggtg aagccggcgg
     7561 tcgaagcggt caaacaagcc gaaccgggca agcccgcggt cgaagccgcc aagcctgcag
     7621 tcgaagccgc aaagtccgca gaagcggtca agccggcaac ggaagcggcc aaacccgcag
     7681 aagcggtcca agccccggcc ccggctgccc cggaaaagtc ggccaccgag tgggcacaca
     7741 cacccgtcga tgcggcacct ccgggtcagc ccgtcgacat ccgcgtccaa atgccggtca
     7801 tgaagggcgt caaggtgaag gtctatttcc gcaaggaagg ccaggccacc ttcgacagct
     7861 tggagctgaa gcgtcgcggc aacgaaaaag tcgcgcgcat tcccgaatcg gtcgcgcaag
     7921 gcaaagcatt ccagtactac atcgaggcca aagacgcggc aggcaccatc gtcaagtcca
     7981 tcggcaacga catgtcgccg aacctggtgc tgatcgatcc gacggcgcgg cctcagatca
     8041 ttggtgccga agtccagggc gaaaccgcgg atgacgatga agtcgccaag cggacgaaga
     8101 cgggcccgac gcgcgacatc gaaaacgaac aggcgacgtt tgacctgggt ggtcagtcgg
     8161 cggcgatggc tcgtctgcgt cagcagatgc gcgaccggga aaagtcgaag agcaaaggca
     8221 gcggcaagtc cctgctcggg ccgatgggct ggggtggtgg tgcggtggcg cttctcggac
     8281 tcggtggact tggggccggc ggcgcgttcc ttgggatggc gtcgaactac gccaacttgg
     8341 tttcgagcga ttcggcctgc gataccaagg tcaaggtcga cggtgttccg cagtgtccgc
     8401 actttggccc gaacgacaac cccgatctgt cgaagacgct caagcctgcg acggcggaat
     8461 atgaacgcaa gggaatgctc tatgacaagg tgggcaccgc ggtgactgcc gttggtggcg
     8521 tccttcttgc gggcggcgcg gcgatgctga cctacgacat catcaagcgc tatggcaaag
     8581 agaagccgcc agcgccgaag acccgcaagg tcaaaaaggt gatcgaggtc gaggagcccg
     8641 ccacgtcgtg gctgattgcg ccggtcgtaa gccccggctc ggtcgggatc gtctcgctgt
     8701 ttaactacta aggtgttgtg ggaggattcc cgatgcgtct ttcgaagctt ttgaccctgg
     8761 ggctgctgct gtcggcggcc tgctataagc caagctacag ctccgacgag gatctgggca
     8821 aagcgttccg ctgctacgtc aacgacaagc ccgcttgccc cggcgatctg gtctgctgcg
     8881 tcggtgacga gtgtggcgac gcgctgctcg atccgatcac cggcatgccg cgcatcgaca
     8941 atggcaccgg tcgtcccctc gaaggctcgt gtgtgaagcc gcgtccgccg aaagacatgg
     9001 tggtcacacc cgtcggttac tgggactttg gcatcaaggg cagctactac accggcatcc
     9061 agaacgatcc gatgctgacc ggcaaagatc ccgacaccgg caagtggcgc tgcaagcgcg
     9121 acgacctcaa catgagcaag cccgccgaga tcgagcggat gttcgagccg aacgacctgc
     9181 ccgatcaggc gatcagcctg tcgaatccgc tgcccatcga tccgacgccg ggccctggca
     9241 cttcgtatga aatttgccca gaccagagct cgcccgacct gcccgatgtc gacgtgttca
     9301 agttccggct gacctcgccg accaaggtga ttgtccgcct gacctacaag gtcagctacg
     9361 gcgaccttga tgtggcgctg ttccggctgg ccaaagacga cgccaccgga gagctgaagc
     9421 cgcagcgcgt gctcgccgac ctgacggcgg tggacaatgg ctgcctcgaa gcgccaaacc
     9481 tccagccgct cggcccgcgt gagtactact acatcgttgt gcgcgggacg aactcgccag
     9541 acaagccgtc cagctactcg atgaacaact acagcattcg cgtgtacggg acgacgaccg
     9601 attcctgcgt cgccaaaaaa gacggcggcg tctagccggc tgcgtttctc tttcccacaa
     9661 cccatagctc cggggaggcg caagcttcct cggtggtgag accatccaga tgcagacctc
     9721 tccgcgtcgg gcgatgacgc ttacgcaaaa gattttggcc gcccatgcca ttgggctttc
     9781 gcgtccgtgg gtaaaggccg gtgacatcct gcgcgtgcgc gtcgactgga ccattgccag
     9841 cgagctggcg tggaacggca tggacaagac ctacgaagcg ctcggtaggc cgcccctgca
     9901 cagccccgat cgtttctttt tggccgtcga tcacaccgtc gatccaacga cgctgcgcag
     9961 cgatgtgcga gcccagaagc tgacccagct ttcgcgcagc tttgccaaag agcgcaagct
    10021 ccgtcacttc tacgacgcca accagaccat cttgcacacc aagttctatc gcgacctgct
    10081 tctgccgggt caggtggtga ttggggcgga ctcgcacacg tcgtcgcatg gcggcctggg
    10141 cgcgtttgcg attggtctgg gcggcgccga tgtgaccgct gcgatggtgc tcggtgagtc
    10201 gtggattgaa gtgcccgagg cgatagcggt cgattacgtc ggcaccccgg cgtttgggat
    10261 gaccggcaaa gacatcatcc tgcgcacgct cggcgatctg ggccgcaaca cggtcgcgat
    10321 ggagcgcagc gtcgaatatc gcggcgaagc ggtgcggaac ttttcgaccg acatgcgctt
    10381 taccatcgcc aacatgaccg ccgagtttgg cggcttcagc ggcatcttcg agcccgacgc
    10441 cgtgactgca gcctggctgg cccagcgcag tgagccgccc aaggccggcg aagcgctgtt
    10501 tttccgcgcg gacgacgatg cggagtatcg gacgcggcac acgattcggc tcgacagcct
    10561 ggaaccgctc gttgccaagc cgttttcacc cgacaacgtc gtgccgcttt cgcagcacca
    10621 gggcatgccg atccatggcg cgttcatcgg ggcctgtacg acgaccgaag aagagctggt
    10681 gctgggggcg ctggtgctgg aagaagcgct gcgtcgcggc attgttccga caggatcggg
    10741 ccgccgtttg gtcgtgcccg gcgatctgtc gattcaggga cggctgtacg aagcgggtct
    10801 tttgcgggtg tacgagcaag ccggcttcca ggtcgatccg ccgggctgct cgatgtgcct
    10861 tggggtcgcg agccagcggg ccggcaaagg cgaaacctgg ctgtcgtcgc aaaaccgcaa
    10921 ctatccaaac cgcatgggcg aaggctcgct ggcctggctg gcatcgggac cggcggtcgc
    10981 agccgcggcc atccagatgc aggttcgcga tccgcgagag cttctcgctg gcatcgaccc
    11041 cgatcgctac cagcgaatct tgcggcgcag cggccacagc ctgcccactg tcaccaccag
    11101 cgagccgacg gtttcgatca gcgctgggac tggatctgcc gcgcacgcca cccaaacagc
    11161 agcggcgcaa accgtgcagg ggcgcgtcca gcgctttggc aaccacgtcg acaccgacgc
    11221 catcatcccc ggccagttct gtcacctgac ggacttttcg gagcttggcg atcactgctt
    11281 cgagttcgtg cgtcccggct ttgcgagtgc cgtccgcgcc ggccaaaaca tcgtcgttgc
    11341 cgaagaaggc tggggctccg gttccagccg tgagcaagcc gtctgggcgc tcaaaggtgc
    11401 gggcgtatcc gtcgtcatcg ccaagagcta cgccttcatc cacaagcgca acctggtgaa
    11461 cgaagcgctg ccgttccttg tcgtcactga cgaagagttc tacagcctgt gcgccgacaa
    11521 cgacgaactg tccgtcgacc tgtcgggcac cgtgcgtcat gttgccagcg ggcggacgtg
    11581 gagcgcccag cccaccaccg cgctcgtatc cgccctggtc cgcgaaggcg gacttgtccc
    11641 cgccatcaaa caccacggcc agaccgtctt cgccaccctc accaccggct aaggccccac
    11701 gcaagaagtg ccggaagtat gtgagcgtgg gggcgaccgg agagcttttt tcggaccgcc
    11761 tgagggacgg aaaaagactc gcagggaagc ggggccccac gcaagacgtg ccggacgtag
    11821 atgatcgagg tcgaggacgt agatgatcgg taacggagtg gtcgatgatc tgccacagcg
    11881 tggtcgatga tctgccacgg agtggtcgat gatctgccac tgagtggccg atgatcggta
    11941 actgagtggc cgatgatcgg taacggagtg gtcgatgatc ggtaacggag tggccgattt
    12001 agtcgagggc cggcagggtg agtcggagct gcgttttcgg tgggcccgcg tctgagactt
    12061 ccacgcgccc cccatgctgt tccgccaccg ccgcaactat cgccagaccc aacccactgc
    12121 cgcccgtgtc acgccgcgtg gtgacaaacc gctcgaacag ccgcccgcga atgtccggca
    12181 aaatccccgc cccccggttt tcgacttcca agatcgcccg ttcctcgtca tggcggagcc
    12241 gcaccgtcac cgccggttcg gctgctacac cgtggctgac atcggcaaac gcgatcgcgt
    12301 tgtccaccaa gttgtccact gcccgacgca gccacggctc gtcaccgggg accatcagct
    12361 cgcccggcag ctcggcacgg acttcgattc gccgctgcgt cgcctgtgcc gcatgggcat
    12421 cgatcgactg acgggtcagc tcggccagat cgacgggttc ccgcaccacc ccgcgcgctt
    12481 gcagccgggt cagcatcagc aggttcccca agatgccctg gagccgctgc gccgcttccg
    12541 caatccgcgc cacaaagcgc cgcgccgtat ccggttcatc gagcgcacca tcggcgagca
    12601 cttccgccgc cgcacgaatc gccgcgaccg ggtttttcag ttcatgcgac agatccgccg
    12661 cgagcttttc cgccaggtgc cgtccttcca gctcgcgccg catcgactcc acggcgtgcg
    12721 tcagcgtccg cacctcgcga ccaaacggcg gtggcagcac cgcttgccgt tcaccttgcg
    12781 caatgcggga cgccgcctgg gtcagttgtt caatcggact cgcaatcgcc cggccaatca
    12841 gcgccgctgc actggccgtc cccagcacca aaagcagcgt cagcagcgcc gcccgcgacg
    12901 ccacttcccg cagaatttgt cgaaagccca ggctttcccg cgaaacccgc accgccccaa
    12961 ggactgcgcc ctgtggcgac cggagcagcg cttgttcaat cggacgctgc ggaatcggcg
    13021 ctccgctggc caaaagcacc cgaccttctg gatcgacgac ttcgatgtgc aggccaccgg
    13081 gccggacctc ggcgagcttt tgcaaagacg gcacatcggt cagatcctgc ggcgtcttgc
    13141 cagtcagcga gatcacagac gacagaatcg cggcttcttc gcgtgatact tcggccaaaa
    13201 gccgcgccgc ccgcagctcc agcctttgca ccgcaagcag tcccaaaagc gccgcgaagc
    13261 cgcccgacac cgtcgccatc gccacaaaca cctgcatccg aatcgacagg ccgtgtcgaa
    13321 acgaacgcaa aagccgccac agcacaaacg cagcaaacag cgtgagcagc agcgtcgcca
    13381 gaaccggcat aaagacgtcc atccggccac gctacaccaa aatgtcgctg tgctaacgtc
    13441 agcgcttgtg aagacaaaaa cccaacttgg tctggctctt gtgctgtcct gtctgcctct
    13501 gcttgctttt gcggacaaag cgccgccgcc gcacgcgcat cacggtgggc cgggcgaaaa
    13561 gccgatggtg catcgcttcg aagatccggc gaagtggacc gcggtgtttg acagtccgga
    13621 ccgtgacggc tggcaaaaac cgcaagagat tgtgaagcgg ctgcagcttt cacccggcag
    13681 cacggtcgtc gacatcggcg ccggcactgg ctacctgatg ccgcacttgg cggcggcggt
    13741 gggcgacaaa ggcaaggtca ttgccgtcga catcgagccc aagatggtcg attacctgac
    13801 cgagcgggcc aaaaaagcca agctcacaca agtcaccgcg cagctcggtc tgccgacgga
    13861 ccccaagctg gctccggcga gcgtcgacaa gatcgtcttt ctggacacct ggcaccatgt
    13921 gccgtcgcga atcgagtatg cacagaagct ctttgcggcg ctgaaaccag gaggcaccgt
    13981 ctatgtcgtc gacttcacca tggagtcgtc gcacgggccg ccgaaaaagc accgcctctt
    14041 gcccgaagct gtgtccctgg agctgtccac cgccggcttt gtcgccgaag tgttgcccga
    14101 agacctcccc gaccaatata tcgtcgccgg aaaaaagaaa gccaacccgt aacttttgat
    14161 cacgctcgga ggtttcatgc ttgcgaaacg tttgctgtcg ctgggtttga cgatggggtt
    14221 ttcgacgctg ctcgcctgtg atccggttcc cccggcggaa acggacacgg caccgctgtc
    14281 gtccgaacag gcgctgcatg ccgggtcggc gattcagcag gtgttcgtgg tgatgatgga
    14341 aaaccagaac gccaaggacg tgtatggcaa ccccgatgcg ccatacatca atcagctgat
    14401 gaaagactac ggctacgcat cgaacttcca agactgtctg ccatcgagcg tcccgagcga
    14461 gccgcactac atctggctgg aagctggcac caacgcattt tccgatgtga cctttggcgg
    14521 cgatggcgat gcgacgccgc gcaacagcac cgcgagcacc gcgcacctcg tcaacctgct
    14581 cgacaaagcc ggcataagct gggcggccta tcaggaaggc atcgattcca ccatcagcgg
    14641 cgcctgtccg atttcgtcca atgggctcac ccgctacgcc gccaagcaca acccgtttgt
    14701 gttcttcaaa gacgtatccg gcaatccccc gagcaagacc gccagccgct gcgtcgccca
    14761 caacaagccg ctgtcccagt ttgcgtctga tctttcgacg ggcagcgtcg ctcgctacaa
    14821 cctgatcacc ccgaacttgt gccacgacat gcacggcgcg ctgggctgct cgggcgggtc
    14881 gaacgtcaag cagggtgaca cctggctgaa aggctttttg ccgtcgctta tcacctatgc
    14941 gaaagccaac cgcggcgtga tccttttgac ctgggatgaa accgagggca caacgacgca
    15001 gccttttttg gtcatcggca gtgggcttaa gcggtctggc tacgaaagca aagtccgtta
    15061 cgacatgtcg tcggtgctga agtcgattca gcgcatcttt gcggtgacgc cgctccttgg
    15121 tcatgccgcc gatgcctcga ccaccgactt gtctgacttc tttgcgacgg gatcgtttcc
    15181 ataaccgatc gcccgcgtgg taaagcggag acgtgagttt gtttgagccg tctctgaccg
    15241 tggtgattcc cgccttcaat gaggcagaca acctaaaagt cgtgcttccc gacgcactgc
    15301 gcttcatgtc cgagcgctgc gtaaactgca ccctgctgct tatcgacgac ggttcaaaag
    15361 acggcaccgc ttctgtcatc gccgagtacg tcgccaacca tccccagcgc gtccaggtca
    15421 tcacccatcc gcgcaacctg gggctgacgg cggcgctgcg cagcggattt ttcggcgccc
    15481 acagcgagct tgtgacctgg attcctgccg acggacagat tccgatttcc gagctcgaca
    15541 agatgctgcg ggcctaccgc ggtgaggatc tgctgctgac gacgtaccgg catcgtcccg
    15601 atggcctgtg gcgggccacg atgtccaaaa cggtgcggat catggtgcgg ctggcgacgg
    15661 gctttgccga tcggctggaa ggtccgtatc tgtttcggcg ggcgctcatc gacgagctga
    15721 acctggtctg catgcggtcc gccggttcga tcgggcttga gattgcagcc aagagccacg
    15781 cgctgggcaa gcggatcgga tcgacggaaa ttgagtgtgt gccgcggctg tcagggcggt
    15841 ccaaggtcgc cgacttgcgc aacatcgtga cgtactttgg cgagatttgg ctgatccacc
    15901 gctcgcagtc cgagctgctc gccagccgtc gctaacaccc ctggttgcgt gcggtccgat
    15961 tgggtctttg tatcgcgcag aaactgctgg acggcgtaca acagcggctg ggcatgtggc
    16021 ggaatgccgg gttcatcgcc gagcccgtca tgcagcaaca gcacccgcgg acggtcggcc
    16081 ttgcgcccaa gcgattgcac gatgtgaagc cgggacagca gcgtggcggg cggtggcgcg
    16141 tcggtgtccc agacgccgtg atcccacatg cacagccgca gatcacttgt gcgcagcgcc
    16201 gaaagcaggg caggggtgcg gacgccgtgg cttggccgaa acagcgtgat gggctgaccg
    16261 gcgatggcca taagccgctc tttggcgcgg agcaggctct gggtcagctg ggtgtgggtc
    16321 tgccacagcg gcaagcgatg atcttcgccg tgcagtccca agacgtggcc ttcacggacg
    16381 atgcgccgga tgatctgcgg atgtgtttcc gccttgtgca gcagcacaaa aaacgacgcg
    16441 cggacaccgt gggccaaaag cagatcgagc agcgcatcgg tcaggcccgc ggtcggtcca
    16501 tcatcaaacg aaaacgcgac ctgttcatcc gaattgccgt cgacaaagcc atgtagatcg
    16561 cgcggcacca gccacagccc gcagggaatc cggcaccaca gaaagccaag cagcgccgac
    16621 aaaaacagca gctcgccgag caagagcagt ccggggtgac tgggtttcgc aagccacggc
    16681 agtccgatca acagcgccgc accacacagc aaggccagga cgcggagctt ggcggcccgt
    16741 ggcacgacgc gaagtgcaga cagcgctcgc ttcatggctt gtgatgactt tggcggcggc
    16801 gcggcgggcg caacagtcca tttgcgacga cggtgaggca gatgagaagc aggctataag
    16861 cgctgcgaag acttcgccac atgccgaagc gatctaccac ttcgcgatag gcgccggcac
    16921 cattttttga gtgatttggc tacgttggac aaatgaagaa tgagcaatgg accgcaacgc
    16981 aggtatttgt cgattcaatg attgaaaacg aagtagccat cacaagataa ttgcgcaaag
    17041 ggagacatcg acttggatcg ctcgacgtcg cggcaggggg gttcgctgct ttcgtggttt
    17101 gcgatctttc atcgtcgcta tcgttccgtg ctgacccagg cgatgctcgc ggggacggcg
    17161 gcttcggggc tggtgtacct cgatgacacg ctgcttggct ggatcaccag ctcgctggcc
    17221 gaccctggcc agggcaaaga aatgccggtg ccgatgctgg tcggtgactt ggcgaaccga
    17281 ttgggcattg gtctaccctt tttgctggtt gcgtttttct ttgtaaatcg cttggttact
    17341 gcgggtattg gtttttggca gatatattgc acgggtgttc tgaatctgcg tggcaaagct
    17401 gatttggaac gtgaaatcct gacgcatctt ttgcgcaaag atgacgcctt ctttggtcgc
    17461 cactcacctg cggaaacggt caatcggttg tcgatcgatc tgcagcgaat ctgcgcgcga
    17521 aggccgaacc tgatgatgtt ttggtggtcg gcgctgctca tcatcggcaa cctgacgtac
    17581 ttcgtggtgc acgatctgcg gctgtcgctg gtggctttgg caagctgcgt gctcgggggt
    17641 ttttgggcca gcacgatgtc gggacgggtc aagcagaccg acgcgcgcta tctgcacgaa
    17701 gacgaccaag tgaagcgctg tttcgaagac tatctgcgtg cgtttcagga agtgcaggtc
    17761 ggcaagctgc aagacaaagt gacggcgcat ctgggacagc ttcaagagca tcgcggccag
    17821 acgtttcgtc gctacgtgat catcaacgag tcggtggcgg tgggaaacct gatggcgaat
    17881 ctgctcgcgt ttgttgcgat gctgctcgtc gtcatttaca tgcgcaaaag cggcgcggtc
    17941 agccgggccc tgatgctgat ccccgtcatc attcacacgc ttccgtatct tatcaagcac
    18001 atggttcagc tcatcaattt gagcatggac tatcagctgt cgcggacgag tgtgcatcgg
    18061 ctgctcgagt atgaaaccga cagcgatgag gagtcgggcg gagccagtgc gaaggatccg
    18121 attccgacca cggtgaagct gcacaacgtc acctatcagt acgctggtcc cgatgggagt
    18181 cggcaaggcg gagtggtcgc agtgcaagcg gagcttctgc ccggaaagct cacgaccatc
    18241 gtcggtggtg ctggttccgg gaagagcacg tttatcaaac tcttgctcgg gcgactggtt
    18301 ccgcaggatg gagcggtaca gtacggtgat gccacgcttg cgacactgca aaagcgcgag
    18361 ctggcggcga tgtgtacgct gatgccgcag acgcttgcgc ttctggatgg gagcatcgcg
    18421 cagaacattc tgtttggtcg tccgcgcggc caagacacgc tggatgcaca agactttgat
    18481 gtggtggaaa agtccggact tgggcggatt tgtcgtctga aagcgctgga aatggcgccc
    18541 gatccgaaga tcgaagtacc cgagctttac cgcgacattg cagcgctgcg ttcgcagctg
    18601 cggcagcggc ttttggaaaa gtgcggagtg acggtgcgcg gctatgaaga aggacacgcc
    18661 gatccaaaac actgggcgct ggaaatgctg cttgggggtc gctgtgatcg ggcgcgcacg
    18721 ctgtcacttc ttttggcgga ctcgacgcgg cggagtctgg cggcgctttt gtccacggaa
    18781 gaaggcaagc tgcttatcga ccgcgcctgc catgtgctgg cagaaagcag cggtcttttg
    18841 tcgctcgaca acttccatgt gtattcgcag ctttccccgt ggccgctgga agaaaaagtc
    18901 tggcagcttc ggcggtcttt tctgcacctg tcggcgcggc gtccagacgg caagcgcgag
    18961 cttctggctg cactgacgat tggactgact tcatcactgg gggaaaacgc agcatcgaca
    19021 gagcagctca agacggtgcg gcgcagcgag caagcggcag gatcgctgcg gcttctgcgc
    19081 gatttgctgg gttcggtgtg tcgtccgttc cagctcgatt cggtgcatcc gtttctttcg
    19141 tggcgcgaaa atctgacatt cggtgtcatc gatctgccga acagccgcgc cggtcgcttg
    19201 gttgaacaga cactgctcca gtttcaggaa caggacggtc tgcgcgaagc gtttacgcgg
    19261 ctcggagtcc agtttggtat cggtcgtcag ggcgcgaacc tgtctggtgg gcaaggccag
    19321 cttgtggcgc tggtgcgggc gcttctgcgc aaaacgcagg tgatcgtgct ggatgaacca
    19381 acgtcggcgc tggacccggc aagccgtgcc cgcgtcgtcg agcttttgac cgattggaag
    19441 aaagatcggg tggtggtgac gatcagccac gatccggagt ttgcgcgaca ggccgatcat
    19501 gtcctcctga tggacggggg acggctgatt gccagcggga cgtttgatga actgaaggaa
    19561 accaacgagg tcttccgaag gacactacga caaacatgaa cgcgatgcct ttgtctgccg
    19621 ctgaaaaaat gaatgtgctg acccgctgcg tggtgttcgc aggcattccg agccgcgatc
    19681 tctctgtgct ggcggaaatg atgacgaccg agcgactgtc tgcgcaggaa gtgctgtttg
    19741 aaaaaggcga cccgtcggat tcggtgttcg tcgtggcaga cggcaagctg ggaatctttc
    19801 tggaccccgc tggaaaaccg gtgcgcatcc tgggccctgg tgagctgctc ggtgaatacg
    19861 gaatgttcat cggcctgttc cggacggcga cggtgcgagc cgacagctcg gcggtgcttt
    19921 tgtcgcttga ttaccagcgg ttccgcgcgt ttttgctcca gtttccgcag tcggcgctgg
    19981 tgctcatgaa aatagccgtc cagcgtctgg tggcggcgga atctgctcgt cgctagatgc
    20041 gccgtcttga ggcttcgctt ttgttgctac gcacccgctt cgcatttgtt cacacagatt
    20101 ccgcagcaaa ttccgcagct tggcgcgggt cgaagttctc tgccgaaagc gtgtcactcc
    20161 gtcgtacagt attgttctct atggagcatt agtccgtgaa cgacctagat gcttggttag
    20221 agcttgacga tgagaaaatt cccttagcgg acgactgctc gatcggacgg acgcagagca
    20281 atacgctgcc gcttcgtgat gacaaagttt cgcgcgcgca cgctcggatt taccgccagg
    20341 aagacgagtt ctgcgtggtt gatctgggca gcgccaacgg cacgtttgta aacggtcgcc
    20401 gcgtcgttca gcccgtgcag ctgcgcagcg gcgacgtggt agcggtcggt ccctttgagc
    20461 tgacattccg tcgcaatgaa aaagccgctt cgattccgcc gcccagcctc gatgaatcgc
    20521 tcaacgtcca gacgctggtg catgtccgtt cggtggactg ttatctgctg gttgcagaca
    20581 tcattggatc gacgcagctt gtgacgacgc acagctcgga tgcgctgccg tttatcaccg
    20641 gacggtggtt tacccgctgt caggaagtca tcgagcgcaa caaaggttcc atcaaccaat
    20701 atctgggcga cggactgttt gcgtactggc agtcgaacaa tgtctcgccg atgcaagtcg
    20761 ccgaaggtct gcgccagctt gcgtatctgc aagcgaccga agaaacacgc ttccgttttg
    20821 tcgttcactt tggccccgtt tcgacggggg gagtggttgc aaccggggaa gcgccgctgt
    20881 ttggacaaga cgtgcactac gtcttccgga tggaaaagct ggcgccacag cttaacctga
    20941 cccgcatgat gagccactct gccaaagagc tgctcgggcg atttttgccg acgagcgaag
    21001 ccggttccca tccgctttcc ggaatgaagg gaacccaccg gatgtactcg ttttaattcc
    21061 tgatcttcct tcgcccccac ggcttgccaa tggggcgttt cacaccgcgg acttggtcag
    21121 cgatggaagc ggtttccatc ccaagcgtag ctttggtttt cgatggcgat ccgcaggttg
    21181 ggggtgccgc gtccgtgctg tgttaggaat cggcggactt cggcgtcgct gtagcgcggg
    21241 tttatgtgtc cagcgaggat cagcttgtcc ggatgttcgc gcgccatgat cccagcgtgt
    21301 tcgatcgatc cgtgtccgaa cgtctgttct ttgatgtgcg gaaagtgcgc gtcgtaaagc
    21361 gccagatgac tgcccaaaag ggctctctct tcggctgcgc gcgtcgcttg ggttggttcg
    21421 tgatcacaca gatatgtcac cgtcggcccc gacgggactg tgacccgata acccagcgca
    21481 tcgagcatct gccgctgggc cccgctgccg ctgtagtgat gaagcgcaaa cggttcgacc
    21541 tgccaaccaa aaagagtcag ccgctggcct tcgtgcagcg tgcgaaattc cagatcagcc
    21601 agcgttccca gcgacaggat ttgcagcggc acaaacgacg gatgacgcag cccctgttcg
    21661 atcgcttgca gcgcttcttt gggcccgcag atgctcagcg aaatgccatt gtctcgtcgc
    21721 caaaaccact ctgcgtcttt gattccttcc cagtgatcca gatgggaatg gctgaccagc
    21781 acgacaacgt ggcggatctg cgcaagccgt ccccgcccaa acagacgggt cgaaagcgcc
    21841 gtcagtccgc gtccggcatc aagcgcaaac agcacatccc catgcgacag ggaatagcag
    21901 gatgtgcagt tcccaatctt ggacatcgcc ggcaggatgt tgcgcgatcc gcgacagccc
    21961 cagatatcaa accggatcga cggagcttcg ccctcggaca gagcgctgtg cggcaaatcg
    22021 ctcgctttgt gcttgggcgc gaccacgacg tctagccatt tcatcacagt cgcagaggtg
    22081 ggggaatgca aaagaagaga cggcgcttcg aggcagtgag tctgcgcgct tacgcagcgg
    22141 ccctggcgcg cgtggacact gccggatgat ggtgataggc tgccgccgcc agtttgtaaa
    22201 agctacttcc aaggagaaga cgacatgcga ctgagtgata tgaaggtgat cgtgaccggc
    22261 ggcgcgcaag gcatgggagc gcactttgtt aagcggctcc acgaggcggg tgcgcaggtg
    22321 gctgctggtg acgtaaacga agcaggtctg gccgagctgc cggcgggcgt gcaccgtcgt
    22381 cgtttgaatg tcgcggacga agccgatgtg caggcgtttg ttgcctgggc acacggcgcg
    22441 atgggcgggc tcaacggtct tatcaacaac gccggaattt tgcgcgacgg cctgctggtc
    22501 aaaaaagaca aggccaccgg cgctgtcatc aagatgtcga cggatcagtg gaacgccgtg
    22561 ctcgccgtga acctgcacgg cgcgaccttc atggtgcgcg aagtcgttgc caaaatggcc
    22621 gaaaccgaca gccgtccggg cgtgattgtg aacatgtcgt cgctcgcgcg gtacggcaac
    22681 cgcggccagt caaactatgt gtcggccaaa gcagcgctgg cggccaatgc ggtgacgtgg
    22741 gcgcgtgagt ttggtccgct cggcattcgc tgtgtcgcga ttgcgccggg catgatcgaa
    22801 accccgatga cgcagggaat gaaccagaaa gcgcgcgatg cactggtcgc ggcggttccg
    22861 gtcggtcgcg ttggtgtgcc ggaagacatc tggcaggcgg tcaagtttgc gatcgagtgc
    22921 gactatttca acggtcgctg tgtcgacgtc gacggcggtc tcgtgatgta agaacgctgc
    22981 gagctacgat cggtcggaat gggatttctg attgagcagc tcgcgaattt tccgcaggag
    23041 ctgcggcgga gcgaacggtt tctgaagaaa cgcatcgact gccgccgcga ctccgtgccg
    23101 ggtgatggca tcatcggtat agccgctcat gtacagcacc cggatgtctg gtcggtgtcg
    23161 acggacttca tcggccagct cgcgtccgcc caggccaggc atcaccacat cggtgagcag
    23221 cagatcaatc ggcttttcgt actctctggc cacttcaagt gcatgctgtc cgccgctggc
    23281 ttcgatgacc gtatagccgt gcagctccag cacttgccgc acgagctttc gcacgacatc
    23341 ctcgtcttcg gcgagcagaa tcgtctctgt tcccgtcgga gaatcgagcc gacttgcagc
    23401 gtgggcggaa accggcagct ttttttgctg cgggagcaga acgcggaacg tggttccttt
    23461 gcccgcttcg ctttcaaacg aaatgtatcc tccggcttgg cgaacgattc cgtacaccgt
    23521 cgccagaccc agccctgaac ctttgccgac ccctttggtt gtgaaaaacg gttcgaaaat
    23581 gcgcgacttc acagcatcgg tgatgccgat accggtgtct gctacccaca gcaaaatgta
    23641 cgaacccgga gccggttcac gatcgcgatc gctcgacggt gctgtgatgt cttgttgcgc
    23701 ggtgccaatg gtcagcttgc ctcctttggg catcgcatca cgcgcgttta aagccagatt
    23761 gatcagcacc tgttcgagct gaccggcatc catcttcact gtcgtcggcc caggccgaag
    23821 ctgggttagc agtgtgatgt cttcgccgat caggcggcgc agcatccgcg tggttcccat
    23881 caccacttgg tttaagtcca aaagtctggg ttcgacaatc gcgcgccgac tgaacgcgag
    23941 aagctgcgcc gtcagcgtcg ctgcgcgttc acctgccgca aggatgtccg tgagtggatc
    24001 gtgcaggaca tcatcgtgtg gaagcttgtg cagcagccgc gaagtctgtc cgtttatcac
    24061 cgtaagcagg ttgttgaagt cgtgcgcaat cccgccggca agttgtccta cggcttccat
    24121 tttttgcgct tggcgcagct gatcttccag cttgcgacgc accgtcaggt cgcgcaccac
    24181 gccggtaaaa tgcggtttgc catttatctg aaacgacgcc accgttattt cggctggaaa
    24241 cgtggttccg tctttccgct gcggtgtcat ttcgcgccgg ttgtcaccga gcagataccc
    24301 gttttgttcg cgccagcggt tcatcgccgc agtgtgactg gcgtgctgtg cgaccggaat
    24361 cagctgcttg atgttttgtc cggtaagctc actcggcgca tagccaagca ctcgctccgt
    24421 tgcagcgttc gccatcacaa tcgatccgtc ttcatcggtt gtgatcacgg catcgctgac
    24481 gctgctgagg atggccttta gtagttctgc ggcgcgctgt tcttcactgc gatcgagcat
    24541 tgcgaccaaa atcgcgacgt tgccacgatc ga
//
LOCUS       DAORGN010000056        24483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_806_length_24483_cov_6.552849, whole genome shotgun sequence.
ACCESSION   DAORGN010000056 DAORGN010000000
VERSION     DAORGN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 24483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24483
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_24483_cov_6.552849"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="PKL17_08450"
     CDS             complement(<1..1492)
                     /locus_tag="PKL17_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNK44797.1"
                     /translation="MSTPEPLNPAQWQAVHHKNGPLLILAGAGSGKTRVITFRLAELL
                     KQGVSAQRLLVVTFTNKAAAELRHRVEQLLHGSPSQDPHLPRLELPRWIGTFHSIGAR
                     LLRSLSSYAGLPSSFPILDEDEQLKVCKELLIEEQIDERRLPPRALRAHIDRAKNQGL
                     LAEDYEGTDYFSDLVRRLYRRYEQRLLQIGAADFGDLLLLPVRIAEREPSIAELLASR
                     FEHVLVDEFQDVNVVQYRLLKILAGKTRNLAVVGDDDQAIYGWRGADVRLLLDFQHDW
                     PDAEIVKLEENYRSSQVILDAAHAVVACNEDRHEKRLYTRRDGGDPIVFFRARDDRAE
                     ARFVVESILRMQAEEGYFPEDFAVIYRTNAQSLRFEETLQAVGLPFSLLGGMRFFERA
                     EIKDVLAYLRLCQNPEDELALRRVINVPARQIGQSTIDKLLARARAEGKSLWQVLKLA
                     AAGDGAFAKKKQKDLAQFVCLLEQLGQRSSELAVSALTVEVLDKTGY"
     gene            1652..2008
                     /locus_tag="PKL17_08455"
     CDS             1652..2008
                     /locus_tag="PKL17_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44798.1"
                     /translation="MSHDLEHKKDPKENPFLAAFKATELGKKKRGSDDDQLNIAAAPI
                     HLGGDSSSKVSAQAAQSAASVMSHWASDQPPIDTLTVADRDRKRKKGMLDAGEKPKDD
                     KLGKQEKDDNKQQGHE"
     gene            2117..4216
                     /locus_tag="PKL17_08460"
     CDS             2117..4216
                     /locus_tag="PKL17_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNK44799.1"
                     /translation="MSNRLALSSSPYLRQHAENPVDWYPWGDEAFARARREDKPVLLS
                     IGYSACHWCHVMAHESFENPETAALMNQSFVCIKVDREEHPDVDQLYQQAHQLLAEGG
                     GWPLTMFLLPSGEPFFGGTYYPPEDRYGRPSFRRILTALAAAYRDEKAKVLEYGQKLL
                     DALREVQQQASGTETSAHLPIDLVQRVAGRLGSQVDRREGGFGHAPKFPNPTALGLLL
                     RSYFRSGEVDDLRPALLTLNKMAQGGIYDHLGGGFARYSTDDKWLVPHFEKMLYDNAL
                     LVRLYAQAKVLLVAQGQDVLAQRYQRVIDETVGWLGRQMSAKDGGFYAALDADSEGVE
                     GKYYVWDYAEVEQLVGPQKAALLSRCYDVIPGGNWHDPHGHGPAETSILHVTDQPRDD
                     DEDALLAEAMATLLAARDKRTPPGTDDKILTSWNALLVSGLATAGQLLAREDLTALAQ
                     TTADFLLSHLRRPDGQLLRTYKDGHAKWAATLDDHAFLADALWQLAQATQDERYLTCA
                     HELTESALRDLYDADSQSFFVGPAETDGVSLVTRPVSLHDSAIPSGVSVMCQNLLRLA
                     AVLPDVRDRYQAIAEACLLRLGERAQRSPMGMAGLIHALDLLTHGVVVTCIIEPADCR
                     DRSLRAMALRSYVPDHFVWIVTEGKPLPQAIAALFDGKTARNGQPTAYVCVGTTCSAP
                     IVEAQALRARLAGQTVP"
     gene            complement(4308..6902)
                     /locus_tag="PKL17_08465"
     CDS             complement(4308..6902)
                     /locus_tag="PKL17_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44800.1"
                     /translation="MKKQRFVWSILPGLFLLIGVVAGYGSGCSGNNVGGDNVDAGTDP
                     NATDDMSTDPNAASCAAVNANCGLISDGQGGTIDCGTCTAPQTCGGGGVFFQCGGSSG
                     GCTPKTATEACAAINANCGELSDGCNGTVSCGTCTNAGETCGGGGTPFVCGKGGGTCT
                     PTTCTALNANCGMIGDGCGGTLNCGNCTVAGDTCGGGGVAGRCGQPKCTPYTNCAQVN
                     ANCGFAGDGCGGIIGGATGPGCGSCPSGQLCGANGMANKCGVPAPTCTNLCKNQVTCD
                     GGSTTLTGTVTAPGHDNTATWGTPDPIPNALVYVPNGTVMPFTSGVTCDQCGSDVTGS
                     PLVSANTGIDGKFTLTNVPCGVPIPLVVQLGRWRRQVTLPAVACCSTTAVPTTSTRLP
                     RNKTEGDIPAIAVVTGSADPMECVLPKIGIDTTEFTDPNGTGRVNFYSANGATISGTT
                     PTAATLFGNLTTMKKYDLIILDCEGAPYDKSAYYNNLLNYTAAGGRIYSTHYGYSFLH
                     GQNQTAPPALNTAWDATATWKVNQASPPNQSAIIDQSFPKGKTFATWLKAVAGGTLGQ
                     IPVTQVRHDFDAVVSPSQQWMYGTQTTTTKFQNFPMHYTFNTPVGAMPANQCGRVMYS
                     DFHVSTGAAGTGTFPGECNLGTGKPLTAQEKVLEYMLFDLTSCIKADIPTCTPKTCAM
                     QGFNCGAQGDGCGGIIANCGTCTAPQICGGGGVPGVCGGGCTPLTCAQLGYTCGTYND
                     GCGGTLSCGTCTSPQVCGGGGAGKCGTGSCMAKTCTQLGLTCGSTGDGCGGTLSCGTC
                     PSGQGCVAGTCKTTGCTPLTVDQACTVNGYNCGPVGDGCGGTVNCGTCPSGQSCGVFG
                     PNKCGVVG"
     gene            complement(7228..8709)
                     /locus_tag="PKL17_08470"
     CDS             complement(7228..8709)
                     /locus_tag="PKL17_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44801.1"
                     /translation="MKSARRVVAGWLWVLCTVLWAGCSWIFDDTAPEMPLVGDPPVME
                     RFLKLNQKTARDAFVVYDASDKPWAVIPELPELPAGIPDGVTLPNLPELIRLVRLDPQ
                     EDPTTAATYPSQYLQLTLRSLYIMVTPQLLDPQSKDKTVFLSRVRPGQPLELFGQFPE
                     GTPVLLVSSNEQVGVMISQSSKSRALRAFRMDGSMETRDIPIPEGVDPSKPFDKGRYQ
                     LDGSGERFIVQDGKDVITVYETRAKGGQLSLGKQARTWILDSRQTALLFCGDGGLVRL
                     PLDGSAAMALDSLPCSQEVFRLTSISGTRVVLYKSGDGLRQLTEDGSSAAIWRMDAIG
                     QLLAVGPSGELLYSTDPALTYGAGIGNGFLGSQQVMERGRRPSWSSDHKRLRYLEWAA
                     RSDSAGDFHSRLFASDEVLRIARNVRSFSEIPDGRVVAVSNSVPKGTHNRIIVVDENA
                     QQARWVAQSSRDYLLILGSSDLLAKVVVGQTGWDIRRVPIPAP"
     gene            complement(8900..9301)
                     /gene="rpsI"
                     /locus_tag="PKL17_08475"
     CDS             complement(8900..9301)
                     /gene="rpsI"
                     /locus_tag="PKL17_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNK44802.1"
                     /translation="MANVQRNAVYATGRRKEAVARVWMMPGSGKVTINHRDIDNYFGR
                     ATSKMVLRQPLELTSQSANFDVYVNVAGGGLTGQADAIRLGITRALMKVNAGFRSALK
                     GAGYVTRDPRCKERKKYGQRGARARFQFSKR"
     gene            complement(9318..9782)
                     /gene="rplM"
                     /locus_tag="PKL17_08480"
     CDS             complement(9318..9782)
                     /gene="rplM"
                     /locus_tag="PKL17_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNK44803.1"
                     /translation="MATPIPSTYLKNPDKAQAERKWHVIDVAGLTLGRAATQIATLLR
                     GKHKTDYTPNIDSGDFVVVLNAQKVHLTGKKLDDKMYHKHTLYLGGLKSRPAREMLAT
                     RSDEVIRRAVWGMLPKGPLGRVIIKKLKVYPGTEHPHSAQQPGKLTLKTMRG"
     gene            10070..11383
                     /gene="tolB"
                     /locus_tag="PKL17_08485"
     CDS             10070..11383
                     /gene="tolB"
                     /locus_tag="PKL17_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNK44804.1"
                     /translation="MRRTLLAAFCVGLPLAMALGLPSAARAQADDVVRFKITNPGQAL
                     YKIAVPKLLGDGESAQVLQEVLSGDLSASGLFKSLDPNSFVADLAKEDLSIAPDSWRT
                     VGAEGVIKAKVTATGGDLAIEFRLYEVIKGDRAVLTKSYRGPAASVRRLAHTFADEVV
                     RYFTGEDSFFGTQIAFARPSGRQQEIVVMDYDGAGQRTLTSNGSQNILPSWHPSGSSL
                     LYTSFVRGTPDLWAIPTSGTKARRVSTRPGLNTGGVFSPDGSKIAVTLSFEGNSEIYV
                     ITPNGDVIKRLTNNPGIDSSPTWSPDGSQIAFVSDRHGSPQIWKMSASGADQTKLTRK
                     GSYNVEPSWNPKPLGGKSLIAFSGRDEKGTMDIFTVDAATGDLQRITENQGANSHPSW
                     APTGRALVFKSSRGGVFVSTADGKTQRPVYRGAAETPIWGPSAKP"
     gene            complement(11401..12099)
                     /locus_tag="PKL17_08490"
     CDS             complement(11401..12099)
                     /locus_tag="PKL17_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNK44805.1"
                     /translation="MTGSFLRQVGLLLWKDVRVELRARELIYATMLFAAIIVLLFSFS
                     FLGGAPPTVEVMAGVLWVAIALSGMLGISRSFEREREADTLRALLLAPIERSALYLAK
                     LLSITLLMLVTSLIVVPGLLLFFQMKVGSAQSWLLFLALLGLGTFGFAAVAALFGASL
                     GRARVREVLLPLLVYPLVVPALLAGSRGTYAVLYGTPEALVDAFRWIKFLIVFDSMSV
                     ILGLWLFEPLCAQE"
     gene            complement(12096..12860)
                     /gene="ccmA"
                     /locus_tag="PKL17_08495"
     CDS             complement(12096..12860)
                     /gene="ccmA"
                     /locus_tag="PKL17_08495"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNK44806.1"
                     /translation="MPADGPAVKQVTLRGIFKDYGTKRALSDVSLTLHAGHVTALLGE
                     NGAGKSTLLGVLAMQIRPTRGTLLFDGQDVATLDGMWLRQQLSLLSHEPRCYADLSAR
                     ENLHFFSKLYDHGSTESQRHTQVGELLARVGLSHAADRPSRTFSRGMVQRLALARAIL
                     HKPTVLLLDEPYTGLDQEGVRLLSSIIADERARGAMIVVISHDFEPLAPLLDVAAVLR
                     RGRLEKTQAYVQGECTRQQLLSLYARAAERPLETSA"
     gene            complement(12850..14448)
                     /locus_tag="PKL17_08500"
     CDS             complement(12850..14448)
                     /locus_tag="PKL17_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44807.1"
                     /translation="MRLSSFLVVFLLFQSLAVAQPMMPDPAQMSGIPRPDPQVAPGTV
                     TVRLIRGAFTSPIVDQEVTLTPASGAPLKARSDAAGRATFSGVPTGTYEARSVLGTIT
                     KSSQPIAVMGAPAPGVRVMLVFPKDAPDAAPNANAAPAAQVGAQAASQAEPAGAPSAA
                     SVPATPAPPADGQVHADSALPAGTLRVFVVDEASKVLSGIPVTLYRRSGPEAQVEKLP
                     AQTTTDAGSVVWAKMATGPAEYLVTISRFGYEQPSEPFHLEGAMGTVLTLVSRPPSQG
                     DDARRKLVISTGSHIIFDLQEEMLSVSEILRLNNPSPQAFDPGAEGLRIPLPEGAIAP
                     QLSPGGPSTLSIDQSSPGNVALVWKGPLPPGESMVQFHFMLRHTGELQFRQPASLAIQ
                     DLRVVIEKRPDLKLDGVTDVQERAWQGHQLLFGLLAGTTEGGQLQFTISGLPAEQRAT
                     RLVGGALAALILAGFVFLAWQGKPGDEDAQQAARQKLISRRDKLLDELLAFDDGPARG
                     ARSREQVMGDLTAVYRALDEADAS"
     gene            complement(14448..15113)
                     /locus_tag="PKL17_08505"
     CDS             complement(14448..15113)
                     /locus_tag="PKL17_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNK44808.1"
                     /translation="MSAASTTISMTWTDSRLLYGGSGVTFAVGLCGFSIALGSLSGPS
                     IALAGAVASLWATGLMLVQSARGLTAVSTEDEHQEAQAAVGRRRRELQREYHLLKRAL
                     KELELDRSMGKLSDDDYSEIRGRYRERAVRVLRQIDQGQSYEQQIERDLLARREALGK
                     KAPLESASGRVSEKPSEVDSRKTENDQRPTVIATACPDCGTRNDLDARFCKNCGRKMG
                     GSK"
     gene            complement(15073..17658)
                     /locus_tag="PKL17_08510"
     CDS             complement(15073..17658)
                     /locus_tag="PKL17_08510"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNK44809.1"
                     /translation="MAQANWVALFGSFVLLCTLVLTTYAGGAAVAGARRQSPQLVRSA
                     IYAGYACTALLSLASALIFFAVLSNDFSLKYVHQNSDAAMPWFYKLTSYWGGLDGSMM
                     FWAWLLSIFSTVAIARNRERHRELMPWVTAILMGIQAFFVSLVVFYKRPFDTFLNEVP
                     AAGKGLNPLLQDFYMATHPPSLYVGYVSASVPFAFCMAALITGNLDDTWLQSVRRWML
                     ICWYFLSQGLILGSLWAYHVLGWGGYWGWDPVENAGILPWFTATAFLHSLMIQERRGM
                     MKVWNVVLVVLTFLLTLVGTFMTRSGIVQSVHAFGNDPELAARFLAFIGFAAVFSFGY
                     LVYRLPLLRSRADLESLLSREFAFLLNNWVLIACAFFILVATLFPTLSEAVTRNRITV
                     GPPFFNRWMAPLGLILLLLTGIGPLMAWRRASQGMLWKQFRYPLVVGAMTIILVASML
                     PWARGKSSFMHEKIQAPVSLICFGLCAMVLMTVMQEFVVGTRARQAGTKLDFLTALVG
                     LLLRNKRRYGGYLVHVGIVLMFFGFAGQTFQAENEVTLARGKSTKIGRYEVLLTSFRS
                     YSDPQKEVTEARLLVKSGGAQFELRPAKWAYRGHEEEPPRTIVATHNSLFEDLYVILN
                     GIDGNESVAGLKLIINPLVLWVWLGFVMLMFGTAIAFLPDRNEPTTKVDGETEHRPSG
                     PSKAAIASLIALVLLGASGPAWATGGGGGDSAMHQSTEGQNFPTPPRNDLERDLRKTI
                     VCMCGCGRQTLAECTCGFAAKERGIIARLIDEGRSREQIIRHFLDKYPGESALVVPQD
                     VGFNRLAWIFPVVIIAAALFALGFLARRWGQQARLSAAASSPLKDQERASYERRLDDD
                     LDDMD"
     gene            complement(17662..18177)
                     /locus_tag="PKL17_08515"
     CDS             complement(17662..18177)
                     /locus_tag="PKL17_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNK44810.1"
                     /translation="MTVKTSSALPSGPSVGPPKASGRSRGLLIVLSVGIIVGGIGLLL
                     SDSSGFEYYKHVDEVAKESGSWQNKRLQLHGYVVPGSIKKRMNHDVQKLEYKFQIENC
                     GAVAEAYFAGVVPDTFKDGAEVVLKGQLHGQQFTANEVMAKCPSKYQQAGGSHPTAVQ
                     RCSRDSAGKGN"
     gene            complement(18244..19368)
                     /gene="moaA"
                     /locus_tag="PKL17_08520"
     CDS             complement(18244..19368)
                     /gene="moaA"
                     /locus_tag="PKL17_08520"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNK44811.1"
                     /translation="MRDGSSCGTTSRSGSSSLHLPIVDGKPAVRTGPGTAAGPLVDGL
                     SRSISYLRISLTDRCNYRCTYCMPDEGIDLAPRADVLSFEEIEHAVEALMRVGVRKVR
                     LTGGEPTVRKDLIDLVQRLSRLGLDDLSLSTNGERLVELAAPLKDAGLHRLNVSVDTL
                     REDRFLQVTRRGNLAQVLAGLDKARAVGFVHTKVNCVAMRGFNDDEFAALCAYCWDRD
                     FIPRFIELMPMSDGTLFYPGTFLPASELRQILTRAFGPLYRAEGEPLLPGVGPARYAA
                     VEYHGALRQVGMISAVTEPFCDTCNRIRLSATGQLHGCLAYDDAIDLRSVLRSGLTAD
                     DLMQRLVQVARGGLSEKRAGHVFSESGCGGPRKHMVSIGG"
     gene            complement(19365..19871)
                     /locus_tag="PKL17_08525"
     CDS             complement(19365..19871)
                     /locus_tag="PKL17_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNK44812.1"
                     /translation="MTPAQQHKAHAPRHVRVYVLTVSDTRTEENDEGGRVCRELVESF
                     GHTVVGKAIVRDEPTLVQAVMGKIADEQSADVIISTGGTGISRRDSTFEAVSSLLEKR
                     LDGFGELFRYLSYAEIGSPAMLSRAVAGLCRGLVIFAVPGSPHAVRLAMEKLIGPELG
                     HLKFEARR"
     gene            complement(20072..>24483)
                     /locus_tag="PKL17_08530"
     CDS             complement(20072..>24483)
                     /locus_tag="PKL17_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44813.1"
                     /translation="DPRDIDALRAVAALYRKLDQPDDLAEILHRLIDSLTLVGGNIEE
                     IREAWAELGKLYSDRIMRPQDAIKAWLKVFEINKRDFRALDALEVLYGAESQWEDAVN
                     VIEKRVKLLEKDSQKIAELLKIGHIWEEKVSDLAAAADAYERVQAIDKTNLEASVQLE
                     GIFRTRKNWNQLADLLLARVEFPDNQPTHRIELLQQVARIYEDNMNDRDSAFVILQAA
                     FREDYSVDSTARELERLASIINKWGDLLGEYTQSVTQIADPKARADLWVKIGRWYAEH
                     LNRIDYAIASQQQALQIDKNHKEALTNLAEAYKKTQQWNELVATLEHHSSIEDDAEKK
                     VAILLDLAGIYENQMQNAEQAIGALRRAISADPTCMPALDSLERLYTAGQSWINLIDI
                     LGRKAKNTDDPDKVIMLKRRIGELYDQRLSDPNKAIISHREILDIDANNLRAMRALEE
                     LYAKVQQAEPYLQILETQLDVVGSDDERISLYEKIAVAWENLFKKVDKAAEAHEKALT
                     FSERHFPSYRQLERLYRILKQWPELVDTYRRHISVSVDGAERMSLYAAMGEVYEVEIK
                     DNDRAIESYSEILAIDPNHQGALIALGRLYERVEDWSRAIDTLSHLVSLVQDRRQQVE
                     LHTRIGGLYEDKLQDQETAEGRFLEALRVDPNYVPAMHKLIALYKGRGDWLKASQLMI
                     RAEEQTPNPLEKIKLLFEIGTILRERLDDEKKGAEFLARVMQLDPEHVGAAEPLAELY
                     FREGRWKELEPILDALVRKADKRDAKEMNNLYFRVAKTADALGNRDKALKYFKMAYDL
                     DSTSLQTLMGRADLLYRLEDWEGAFKLYQTVLVHHREAQSDREVVDIFYRLGIIKLRQ
                     TEKKKALNMFEKALELDPNHRPTLLAVIEMQLANNDWEAVISAKRALLPVSEAEEKFK
                     IDCEMAEMYADKLQQYPKAIQSYLDAIEIKPDSHVTLHKVLELYTKTEQWKKAIEILG
                     RLAQLEHDLLRRGKFYYTAGVIYRDALKSTDEAIDQFNLALDSYFSKPELIPSARFQE
                     FLKPFEAIDKICTTRKDWKAQERNYRKMIKRMPKTGQEQVTVALWHALGEIYRSRQKD
                     IPAAIQTFEVAVGLEPENLKRREILGELYIVGGPDYLDKAVNEQMFLIDKDPYRVEAY
                     KALRRIYMDSRQFDKAWCMCSALTYLQRADADEQQFYEQYKKGLPKARNKLTDEMWAK
                     YIYHKDEDRFIGAIFATVYSAVGNLRAAEHKQYGLKRKEKRDLSSDQTMFGRVFTYVM
                     QVLNVPPNVEIFFRTEQPGDLVVANCKEKNLFIPSIVVGQGMLAGRGDKDVAFPSAVF
                     LTKMRPEHYLRAILQSNTELAVALLSAVRLARPDFPVPPQHAQFVDQFSGAMGPFVPF
                     AAREHLGLVVQKYIANKSQLDMAKWAQAVDLTSHRAGLVVCNDLSVAARYIGQEPTAV
                     GGMAPKDKVKELVLYSISPTYFELRALLGIAIG"
BASE COUNT         4913 a         7773 c         7198 g         4599 t
ORIGIN      
        1 gatagccggt cttgtcgagc acttcgacgg tcagcgccga cacagcaagc tcactcgatc
       61 gctgcccaag ctgctccaag aggcagacaa actgcgccag gtctttttgc tttttcttgg
      121 caaacgcacc gtcaccagcc gccgccagct tgagcacttg ccacagagat ttcccctcag
      181 cgcgcgctct ggccaaaagc ttgtctatcg tgctttgccc gatttggcgc gccggcacat
      241 tgatcacccg ccgcagcgcc agttcgtctt cggggttttg gcacagccgc agatacgcaa
      301 gcacgtcctt tatctcggca cgctcgaaaa agcgcatgcc cccgagcagc gaaaacggca
      361 gcccgaccgc ttgcagggtt tcctcgaagc gcagcgactg cgcgttggtg cggtaaatca
      421 ccgcgaagtc ttcgggaaaa tagccctctt cggcctgcat gcgcaggatg ctttcgacga
      481 caaaccgcgc ttcggcgcga tcatcgcggg cccgaaaaaa cacgatcgga tcgccaccgt
      541 cgcgccgcgt atacagtcgc ttttcgtgcc gatcttcgtt acacgccacc accgcgtgcg
      601 ccgcgtccaa aatcacctgc gacgatcggt agttttcttc caacttcacg atctcggcgt
      661 cgggccagtc gtgctgaaaa tcgagcaaaa gtcgcacgtc cgcgccgcgc caaccgtaaa
      721 tcgcctgatc gtcatcgccg accacggcca ggttgcgcgt cttgccggcc aggattttca
      781 gcagccgata ctgcacgaca ttgacgtctt gaaactcatc gaccaagacg tgttcaaagc
      841 gcgaggccaa cagctcggca atcgacggct cgcgctcggc aatccgcacc ggcaaaagca
      901 gcagatcgcc aaaatccgcc gccccgattt ggagcaagcg ctgctcatag cgacggtaca
      961 ggcgacgaac caggtccgaa aagtaatcgg tcccttcgta gtcttcggcg agcagacctt
     1021 ggtttttggc gcgatcgata tgggcccgca gcgcccgcgg tggcaagcgc cgctcatcga
     1081 tctgttcttc gatcagcagc tctttgcaga ccttgagctg ttcatcttca tcaagaatcg
     1141 gaaagctcga cggcagaccc gcatagctcg acagactgcg caaaagccgc gcgcctatcg
     1201 agtgaaacgt cccaatccag cgcggcagct cgagccgggg caggtgcgga tcttgcgacg
     1261 gcgatccgtg caaaagctgc tcgacgcgat gtcgcagctc cgccgccgcc ttgttggtaa
     1321 acgtcacgac cagcagacgc tgcgccgaaa cgccttgttt caggagctcc gcgagccgaa
     1381 acgtgatcac ccgcgtcttg cccgaaccgg ctcctgccaa aatcagcagc ggaccgtttt
     1441 tatgatgaac ggcttgccac tgcgcggggt taagcggctc aggcgtagac ataggctgtg
     1501 gggttatgcc ccaagaagct gggcggatac aagggaagtg cagaaccgac acagtcgaca
     1561 gaaaaaacgc gaaacagcga cgaaccgcac ccttttttca gactgtccga taatttgaaa
     1621 cagcatcccc gccgatgcga aggtgaacgc gatgtcgcac gatcttgagc acaaaaaaga
     1681 tcccaaggaa aacccgttcc tggctgcgtt caaagccacc gaactgggca aaaaaaagcg
     1741 tggttccgac gacgaccagc tcaacatcgc ggctgccccg attcacctcg gtggcgattc
     1801 atcgagcaag gtgtccgcgc aagccgcgca gtccgcagcg tcggtgatgt cgcactgggc
     1861 gagtgaccag ccgccgatcg atacgctgac ggtcgcggat cgggatcgca agcgcaaaaa
     1921 aggcatgctc gacgcgggcg aaaaacccaa agacgacaag ctcggcaagc aagaaaaaga
     1981 cgacaacaag cagcagggtc acgaataacg acctgcgcag actgtttttc ccgcctcgat
     2041 cggttttccc acccaagcga ctcggcacgg catccaaagc gggttagctt cttcccgcta
     2101 ccgcgaggtc tttgtcgtgt caaaccgtct tgccctgtcg tcgagtccct atcttcgtca
     2161 acatgccgaa aaccccgtcg attggtatcc ctggggggac gaagcgtttg cccgcgcccg
     2221 tcgtgaagac aagccggtgc ttttgtcgat cggctattcg gcgtgtcact ggtgccatgt
     2281 catggcccac gaatcattcg aaaacccaga gaccgcggcg ctgatgaacc agtcgttcgt
     2341 ctgcatcaaa gtcgatcgcg aagaacatcc cgacgtcgac cagctgtatc agcaagcgca
     2401 tcagcttctg gccgaaggcg gcgggtggcc gctgacgatg tttcttttgc cgtcgggcga
     2461 gccttttttc ggtggcacct actacccgcc cgaagatcgc tacggtcggc cttcgttccg
     2521 gcgaatcctc actgcgctgg ctgcggcgta tcgcgatgaa aaagcgaaag tgctcgaata
     2581 tgggcaaaaa cttctcgatg cgctccgaga agtgcagcag caagcgagcg gcactgaaac
     2641 aagcgcgcac ctgccaatcg atctggtgca gcgagtggcg ggacggctcg gatcacaggt
     2701 tgatcgtcgc gaaggcggtt ttggtcatgc gcccaagttt cccaatccga cggcgctggg
     2761 actgctcttg cgatcgtatt ttcgcagcgg tgaagtcgat gatctaaggc cggcgctttt
     2821 gacgctcaac aagatggcgc agggcggcat ttacgatcac ctgggcggcg gttttgcgcg
     2881 ctattccacc gacgacaagt ggctggtgcc gcactttgaa aaaatgctct acgataacgc
     2941 gctgcttgtg cgtctgtatg cgcaggccaa ggtgctgctc gtcgcgcagg gtcaagacgt
     3001 gctggcgcag cggtatcagc gcgtgatcga cgaaacggtc ggctggctcg ggcgacaaat
     3061 gagcgccaaa gacggtggtt tttacgcggc gctcgatgcc gacagcgaag gcgtcgaagg
     3121 caagtactac gtctgggact acgccgaagt cgagcagctt gtcggaccgc aaaaagcagc
     3181 gcttttgagc cgctgctacg acgtgattcc cggcggcaac tggcacgatc cacacggaca
     3241 tggcccggca gaaacgtcaa ttttgcatgt caccgaccag cctcgcgatg acgacgaaga
     3301 tgcgctgctg gccgaagcga tggcgactct tctggccgcg cgcgacaaac gaactccgcc
     3361 gggaacggac gacaaaatcc tgaccagctg gaacgcgctt ctggtttcgg gtttggccac
     3421 cgcggggcag ctgctcgcgc gggaagacct gacggccctg gcacaaacga ccgccgattt
     3481 tttgctttcg cacttgcgcc gcccagacgg acagctgctg cgcacctaca aagacggaca
     3541 tgcgaagtgg gcggcgactc tcgatgatca cgcctttttg gccgatgcgc tgtggcagct
     3601 cgcccaagcg acgcaggacg agcgctacct gacttgtgcc cacgagctga cggaatcggc
     3661 gctgcgcgat ctgtacgatg ccgattcgca gtcgtttttt gtcgggcccg cggaaaccga
     3721 tggcgtgtcg ctggtgacac gcccggtgag cctgcatgac agcgcgatcc catcgggtgt
     3781 ttcggtgatg tgccaaaact tgctccggct ggctgcggtc cttccggatg tgcgtgatcg
     3841 ctatcaagcg attgccgaag cttgtctttt gcggctcggt gagcgggcgc agcgcagtcc
     3901 gatggggatg gccggactga ttcacgcgct cgatctgctg acacacggcg tggtggtcac
     3961 ttgcatcatc gagccagccg attgtcgcga tcggtccctg cgagcgatgg cgctgcgcag
     4021 ctacgttccc gatcactttg tctggatcgt caccgaaggc aagccactgc cacaagcgat
     4081 cgccgcgctg ttcgatggca agaccgcgcg caatggtcag cccaccgcgt atgtctgcgt
     4141 cggaacaacg tgcagtgcac cgattgtgga agcgcaggcg ctgcgtgctc gcttggcagg
     4201 acagacggtc ccatagcgct gggctccttg ggggagccgc agctctgggt tttgcaggaa
     4261 ggaaggcccg cggtcaggaa gctacgcggg cccacggagt acgccggtta gccgacgacg
     4321 ccgcacttgt tcgggccgaa cacgccgcag ctctgacccg acgggcaggt gccgcagttt
     4381 acggtgccac cgcagccgtc accgacgggg ccgcagttgt acccgttcac agtgcaagcc
     4441 tggtcgacgg tcagcggggt gcagccggtc gtcttgcagg taccggcaac gcagccctga
     4501 cccgacgggc aagtgccgca gcttagcgtg ccaccgcagc cgtcacccgt cgaaccgcac
     4561 gtcagaccaa gctgggtgca ggtctttgcc atgcagcttc cggtgccaca cttgccagcg
     4621 ccgccaccgc cacaaacctg cggagaggtg caagtgccgc agcttagcgt gccaccgcag
     4681 ccgtcgttgt aggtgccgca ggtgtagcca agctgggcgc aggtcagagg cgtgcagcca
     4741 ccgccgcaga cgcccggaac gccaccacca ccgcaaatct gtggcgccgt gcaggtgccg
     4801 cagtttgcaa tgatgccacc gcagccgtcg ccctgcgcac cgcagttgaa gccttgcatc
     4861 gcgcaggtct tcggcgtgca cgtcggaatg tcagccttga tgcacgaggt caggtcaaac
     4921 agcatgtact cgagcacttt ttcctgcgcg gtcagcggct tgccggtgcc aaggttgcac
     4981 tcgccaggga acgtgccggt gccggcagcg cccgtgctga cgtggaagtc gctgtacatg
     5041 acgcggccac actggttcgc tggcatcgcg ccgaccggcg tgttgaaggt gtagtgcatc
     5101 gggaagttct ggaacttcgt cgtcgtcgtc tgcgtaccgt acatccactg ctgcgacggc
     5161 gagaccacgg catcgaagtc atggcgaacc tgggtcactg gaatctgacc gagcgtgcca
     5221 cccgcgacgg ccttgagcca cgtcgcgaac gtcttgccct tcgggaagct ctggtcgatg
     5281 atcgcgctct gattcggggg cgaggcctgg ttcaccttcc acgttgccgt cgcgtcccac
     5341 gcagtgttca gtgccggcgg tgccgtctgg ttttggccgt gcaagaacga atagccgtag
     5401 tgcgtcgagt agatgcgacc acccgcggcg gtgtagttca gcaggttgtt gtagtacgcc
     5461 gacttgtcgt aaggcgcacc ctcgcagtcg aggatgatca ggtcgtactt cttcatcgtc
     5521 gtcaggttgc caaacagcgt cgccgccgtc ggggtcgtac cgctgatcgt cgcaccgttc
     5581 gcagagtaga agttgacgcg gcccgtgccg ttcggatcgg taaactcggt ggtgtcgatg
     5641 ccgatcttcg gcagcacgca ctccatcggg tccgccgagc cggtgacaac cgcgatcgcc
     5701 ggaatgtcgc cttccgtctt gttgcgcggc agacgggtcg aagtcgtcgg caccgccgtc
     5761 gtcgagcagc aagcaactgc cggcagcgtg acctgacgac gccaacggcc aagctgaacc
     5821 accagcggaa tgggcacacc gcaggggacg ttggtcagcg tgaacttgcc atcaatgccg
     5881 gtgttggcgc tcaccagcgg cgagcccgtc acgtccgatc cgcactgatc gcaggtcacg
     5941 cccgacgtaa acggcatcac cgttccgttc gggacgtaga caagcgcgtt cgggatcggg
     6001 tccggagttc cccaggtcgc cgtgttgtcg tggcctggcg ccgtcaccgt tccggtcagc
     6061 gtcgtcgagc caccatcaca agtcacctgg tttttgcaaa gattcgtaca cgtcggggcc
     6121 ggcacgccgc acttgttggc catgccgttg gcaccgcaga gctgaccgct cgggcacgaa
     6181 ccacagcccg gcccggtcgc accgccgatg atgccgccgc agccgtcgcc cgcgaagcca
     6241 cagttggcgt tgacttgcgc gcagttggtg tacggggtgc acttgggctg gccacagcga
     6301 cccgccacgc caccaccgcc acaggtatca ccagcgacgg tacagtttcc acagttcagg
     6361 gtgccaccgc agccatcgcc gatcatgccg cagttcgcat tgagcgccgt gcacgtcgtc
     6421 ggagtacagg taccgccgcc cttgccacag acgaacggag tgccaccgcc accacaggtc
     6481 tcgccggcgt ttgtacaggt tccgcagctc acggtgccgt tgcaaccgtc ggacagctca
     6541 ccgcagttgg cgttgattgc ggcgcatgcc tcggttgccg tcttgggagt acagccaccc
     6601 gacgaaccac cgcactggaa gaagacgccg ccgccgccac aggtctgggg cgcggtacag
     6661 gtaccgcagt cgatcgttcc accttggccg tcgctgatca ggccgcagtt ggcgttgact
     6721 gcggcgcagc ttgccgcgtt tgggtcggtg ctcatatcgt cggtggcatt cggatcggtg
     6781 cccgcatcga cgttgtcgcc acccacgttg ttgccggagc agcccgagcc gtagcccgca
     6841 accaccccga taagtaagaa caatcccggt aagatagacc aaacaaagcg ttgcttcttc
     6901 atgtgccccc ctttcgtcgt cgtcctttcg atcataaatc gacagaggag gccaatgtca
     6961 atcgaatagc cgctttcgaa tttcccactt acgacgccag cggttatgta gtcagcgcaa
     7021 caaccgaggc tgacacgttc caaggagcat ccccacccac gttgttctca acacgcgccc
     7081 atttcgcatt gagaaattaa gcactcattc aaaatcagcc aaaatttgaa attaataatc
     7141 aaaacaaatc aacacaaaac cattccagat atcacatggc caaacttctg atcgatgggg
     7201 atacatatct tccatgcggt ttcccgctta cggcgccggg atcgggacgc ggcggatgtc
     7261 ccacccggtc tggccgacca caaccttggc cagcagatcg ctagacccca ggatcagcag
     7321 gtagtcgcgc gagctctggg ccacccaccg ggcctgctgg gcattttcgt cgacgacgat
     7381 gattcggttg tgggtgcctt tgggaacaga attgctcacc gccacgacac gcccgtcggg
     7441 gatttcggaa aaactgcgca cgttgcgggc gatccgcagc acttcatcgc ttgcgaaaag
     7501 ccgcgagtga aaatcgcccg cactgtcgga acgcgcggcc cattccaagt agcgcagacg
     7561 cttgtggtct gacgaccagg aaggacgccg cccccgctcc atcacttgct gcgagccgag
     7621 gaagccgttg ccaattcctg ccccataggt cagcgccgga tcggtggaat aaagaagttc
     7681 gccacttggg ccgaccgcca gaagctgacc tatcgcatcc attcgccaga tcgccgcgga
     7741 gctaccgtcc tcggtcaact ggcgcagccc atcgccgctt ttgtacagca ccacccgcgt
     7801 cccggatatc gaagtcaggc gaaacacctc ctgcgaacac ggcagcgaat cgagcgccat
     7861 cgcagcgctc ccatcgagcg gaagacgaac aagaccaccg tccccacaaa acaacagcgc
     7921 ggtctggcgg ctgtccaaaa tccacgtccg cgcctgtttg ccaagtgaaa gctgcccgcc
     7981 tttggcccgc gtttcgtaga cggtgatgac atctttgccg tcttggacga tgaagcgctc
     8041 gccgctgcca tcaagctgat agcgcccttt gtcaaacggc ttggaagggt ccacgccttc
     8101 aggaatcggg atgtcgcgcg tctccatcga gccgtccatg cgaaaagcgc gcagcgctcg
     8161 gctcttgctg ctttgcgaga tcatcacccc aacctgctca ttcgaactga ccagcagcac
     8221 gggcgttccc tcgggaaact ggccaaacag ctcgagcggc tgccccggtc gaacccgcga
     8281 aagaaacacc gtcttgtctt tgctctgcgg gtccagaagc tgcggcgtca ccatgatgta
     8341 caaagagcgc agcgtcagct ggagatactg cgacggataa gtcgccgctg tcgtcggatc
     8401 ttcctgcgga tcaagtcgaa ccagccgaat cagctcgggc agattgggaa gagtcacccc
     8461 gtcggggatg ccggcaggaa gctcgggtag ctcgggaatc accgcccacg gcttgtcgct
     8521 tgcgtcgtag accacaaacg catcacgcgc cgtcttctgg ttcagcttaa gaaaccgctc
     8581 catgaccggc ggatcaccga cgagcggcat ctcgggtgcg gtgtcgtcga aaatccagct
     8641 acagccagcc cacagcacgg tgcaaagcac ccacagccag ccagccacca ctcgcctagc
     8701 cgatttcata tccgcgagtc taccctgcac cgcaagaagc gaaagcatgc gcaaacgcgc
     8761 cacgcaagct gcgtggtcgt cgaaaagtgt gggaaaagaa gagggagaaa aaggaaaaca
     8821 cagcgacaac ccatgtcggg ctgcgctgtg ttctgttttt ggatggcaac cctccgcagc
     8881 gaaagcggag ggattttggc tagcgcttcg agaactggaa gcgagcgcgt gcaccacgct
     8941 gaccgtactt cttgcgctcc ttgcagcgcg ggtcgcgggt gacgtagcca gcgcccttga
     9001 gcgccgagcg gaagccagcg ttcactttca tgagcgcgcg ggtgatgccg aggcgaatcg
     9061 catcggcttg gcccgtcaga ccgccgccag cgacgttgac atagacgtca aagttcgccg
     9121 actgcgaagt cagctcgagc ggctgccgaa gcaccatctt cgaggtcgcc cgaccgaagt
     9181 agttgtcgat atcacggtgg ttgatggtga ccttgcccga gccgggcatc atccacacac
     9241 gcgccaccgc ctctttccgg cgtcccgtgg cgtacacagc gtttcgttga acgttcgcca
     9301 tttctcaaaa aaccttccta gccacgcatg gtcttcaagg tgagcttgcc gggctgctgg
     9361 gcgctgtgcg gatgctcggt gcccggatag accttgagct tcttgatgat gacccgtccg
     9421 agcgggccct tcggcagcat gccccacacc gcgcgacgaa tcacttcgtc cgagcgggtc
     9481 gccagcatct cgcgagctgg acgggacttg agaccaccga ggtacagcgt gtgcttgtgg
     9541 tacatcttgt cgtcgagctt tttgccggtc aggtgaacct tctgggcatt gagcacgacc
     9601 acgaagtcgc cgctgtcgat gttgggggtg taatcggtct tgtgcttgcc gcgcaggagg
     9661 gtggcgatct gcgtcgccgc gcgacccaag gtcaggcctg ccacgtcgat gacgtgccac
     9721 ttgcgctcag cctgcgcctt gtcgggattc ttgaggtacg tgctcggaat cggagtagcc
     9781 atgcgagttc cttagagaga ttggaactat cggatcacaa aagcagtgtc aagaaccttc
     9841 ttaggttgtg cggacttagg cccacgcgcc tgctgaatgc acaccgcttt tggtagccgt
     9901 tttttccggt ctggccggtt cgtttttgga cccaggacgc tagcagattt gggcggggat
     9961 cggtacagcc tgtgcaaact tttttccgca agcttttcag aacatggcgc accgggtagg
    10021 ccacgctcgc cgcttggatt ttcccgcaag ggacgctacg gtaaggccta tgcgacggac
    10081 cctgctcgcg gctttttgtg tgggactgcc tttggcgatg gcgcttggtc ttccgtctgc
    10141 ggcacgggcc caggcggatg atgtggtgcg gtttaaaatc acaaaccccg gacaagcgct
    10201 ctacaagatc gccgtgccga aactgctggg ggacggcgaa agtgcgcagg tgctgcaaga
    10261 agtgctgagc ggcgacttga gtgcgtccgg attgtttaag tcgctcgatc cgaactcgtt
    10321 tgttgccgat ctcgccaaag aagacctgtc gattgcgccg gattcgtggc gtaccgtcgg
    10381 tgccgaaggc gtcatcaaag cgaaagtcac cgcgacgggc ggcgatctcg cgatcgagtt
    10441 tcggctctac gaagtcatca aaggcgaccg cgcggtcctg accaaaagct atcgtggacc
    10501 tgccgccagc gttcgccggc ttgcccatac gtttgccgac gaagtggtgc gctatttcac
    10561 cggcgaagat tcgtttttcg gcacccagat cgcgtttgcc cgcccgagcg gtcgccagca
    10621 agagatcgtg gtaatggact acgacggtgc gggccagcgg acgctcacgt cgaacggctc
    10681 acagaatatt ttgccgtcgt ggcatccatc gggaagcagc ttgctctata ccagctttgt
    10741 ccgcggcacc cccgatctgt gggcgattcc gacgagcggc accaaagccc gacgggtgtc
    10801 gacacggcca ggactgaaca ccggcggcgt cttttccccc gacggcagca agatcgcagt
    10861 cacgctttct ttcgagggaa acagcgaaat ctatgtgatt acgccaaatg gcgatgtgat
    10921 caagcgactg accaacaacc ccggcattga tagctcgccg acctggtcgc ccgacggcag
    10981 ccagatcgct tttgtgtccg accgccacgg cagcccgcag atttggaaga tgtccgcgtc
    11041 gggtgccgac caaaccaagc tcacccgcaa aggcagctac aacgtcgaac cgtcgtggaa
    11101 tcccaagcca cttggcggca agtcgttgat cgctttttcg gggcgcgacg aaaagggcac
    11161 gatggacatt ttcaccgtcg atgccgccac gggtgatctt cagcgcatca ccgaaaacca
    11221 gggcgcaaac agccacccaa gctgggcacc gaccggacga gcgctggtct tcaaatcgag
    11281 ccgcggcggt gtgtttgtat cgaccgctga cggcaaaacc cagcgtccgg tctaccgcgg
    11341 cgcagccgaa accccgatct ggggcccgtc cgccaagccc taaccgccct tttcgctcgc
    11401 ttactcttgg gcgcacagcg gctcgaacag ccacagcccc aaaatcaccg acatgctgtc
    11461 gaagacgatc aggaatttga tccagcgaaa cgcatcgacc agcgcttccg gcgttccgta
    11521 gagcaccgca tacgtgccac gcgagcccgc cagcagcgcc ggcaccacca gcggatagac
    11581 gagcagcggc aaaagcacct cacgcacacg cgcccgaccg agcgatgccc caaacagcgc
    11641 cgccaccgcc gcaaacccga acgtgcccag cccaagcagc gccaaaaaca gcagccacga
    11701 ctgcgccgac ccgactttca tctgaaaaaa caagagcagg cccggaacca cgatcagcga
    11761 cgtgaccagc atcagcagcg taatcgacag cagcttggcc agatacagcg cactgcgttc
    11821 gatcggggcc aaaagcagcg cgcgcagcgt atcggcctcg cgttcccgct cgaagctgcg
    11881 cgaaatcccg agcatccccg acagcgcgat cgccacccac agcacgcccg ccatcacttc
    11941 gaccgtcggt ggcgcaccac ctaagaacga aaatgaaaac agcagcacga tgatcgcagc
    12001 aaacagcatc gtcgcgtaaa tcagctcacg ggcgcgcagc tcgacgcgca cgtctttcca
    12061 cagaagcagc ccgacctgcc gcaaaaacga tcccgtcatg ccgatgtctc cagcggacgc
    12121 tcggcggcac gcgcatacag cgacagaagc tgctgacggg tacactcgcc ctgcacatag
    12181 gcctgggtct tttccagccg accccgccgc agcaccgccg ctacatcgag cagcggcgcc
    12241 agcggttcaa agtcatggct tatcacgacg atcatcgcgc cgcgcgcccg ttcgtcggcg
    12301 atgatcgacg acaaaagccg cacgccttct tgatcgagcc ccgtatacgg ttcgtcgagg
    12361 agcagcaccg tcggcttgtg caaaatcgcc cgcgccagcg cgagccgttg caccattcca
    12421 cgcgaaaagg tccggctggg ccgatcggca gcatgggaaa gcccaacgcg cgcaagcagc
    12481 tcgcctactt gcgtgtgccg ctgcgattcg gtgctgccgt ggtcgtacag cttggaaaaa
    12541 aaatgcaggt tttcgcgcgc tgacaaatcg gcgtagcagc gtggctcgtg cgaaagcagc
    12601 gaaagctgtt gccgcagcca cattccatcg agcgtggcga cgtcctggcc atcgaacagc
    12661 agcgtgccgc gggtcggtcg aatctgcatc gccaagaccc cgagcagcgt cgatttgccc
    12721 gcgccatttt ccccgagcag cgccgtcaca tgcccagcat gaagggtcag cgacacatcg
    12781 ctgagcgcgc gttttgtgcc gtagtctttg aaaatcccgc gcagcgtgac ctgcttcacc
    12841 gccgggccat cagctggcat cggcctcatc cagcgcgcga tacaccgctg tcagatcgcc
    12901 catcacttgc tcgcggctgc gcgctcctct ggctgggcca tcgtcgaaag caaggagctc
    12961 atcgagcagc ttgtcacgtc gcgaaatcag cttctgtcgc gccgcttgct gggcatcttc
    13021 gtcgcccggc ttgccttgcc aggccaaaaa gacaaatccc gccaggatca gcgccgccag
    13081 cgcaccgccg acaagccgcg tcgcccgctg ctctgccggc agtcccgaaa tcgtaaactg
    13141 aagctgtcca ccttcggtcg tccccgcgag cagaccaaac agaagctggt gcccttgcca
    13201 cgcgcgctct tgcacatcgg tcacgccgtc gagcttcaga tcgggacgct tttcgatgac
    13261 gacgcgcaga tcctgaatcg ccagactcgc cggctgccga aactgaagct cgcccgtgtg
    13321 gcgcagcatg aaatgaaact gcaccatcga ttcgcccggc ggcagcggtc ctttccacac
    13381 cagcgcgacg ttgcccggcg agctttgatc gatgctcagc gtcgatggac cgcccggcga
    13441 aagctgtggc gcaatcgccc cttccggcag aggaattcgc agcccttcgg cccccggatc
    13501 gaacgcctgc ggcgacggat tgttgagccg cagaatttct gacaccgaca gcatctcttc
    13561 ttggaggtcg aaaataatgt gcgatcccgt cgagatgacc agcttgcgac gcgcatcgtc
    13621 accctgcgaa ggtggccgag acacaagggt cagcaccgtc cccatcgcac cttccagatg
    13681 gaatggctcc gacggctgct catagccaaa gcgactgatc gtgaccagat actcggcggg
    13741 gccagtcgcc atcttcgccc acacgacgga acctgcgtca gttgtcgttt gcgcagggag
    13801 cttttcgacc tgggcttcag ggccactgcg acggtacagc gtgaccggaa tgccagacag
    13861 cactttgctg gcttcatcga caacaaagac ccgcagcgtg cccgctggta gcgccgagtc
    13921 cgcgtgaact tgaccgtcag caggcggagc aggagttgca ggaaccgaag ccgccgaagg
    13981 agcgcccgcg ggttccgcct gcgaagcggc ctgcgcaccg acttgggccg ctggggccgc
    14041 gttcgcgttg ggcgcagcat cgggcgcatc tttgggaaac accagcatca cccgcacgcc
    14101 aggcgccgga gcccccatca ccgcaatcgg ctgcgacgac ttggtgattg tcccgagcac
    14161 cgagcgtgct tcgtaggtgc cggtcggaac gccggaaaaa gtcgcccgtc ctgccgcgtc
    14221 gctgcgcgct ttgagcggag cccccgaagc aggcgtcagc gtgacttctt ggtcgacgat
    14281 tggacttgtg aacgcgcccc gaatcagccg caccgtcacg gttcccggtg ccacctgcgg
    14341 atcgggacgt ggaatgcccg acatctgcgc cgggtccggc atcatcggct gggccaccgc
    14401 cagcgactga aacagcaaga acacaaccag gaaagacgag agccgcatca cttgctgcct
    14461 cccatcttgc gaccgcagtt tttgcaaaag cgcgcgtcga ggtcattgcg cgtgccgcag
    14521 tcgggacaag cggtcgcaat caccgtcggt cgctggtcgt tttcggtctt acgcgaatcc
    14581 acttctgacg gcttttcaga gacgcgacct gaggcgcttt ccagcggcgc ttttttcccg
    14641 agcgcttccc gtcgcgccaa aagatcgcgc tcgatctgct gctcatagct ctggccttga
    14701 tcgatctgcc gcaaaacccg cactgcccgt tcgcgatacc gaccacgaat ttcgctgtag
    14761 tcgtcgtcgg acagtttgcc catcgaacgg tccagttcca gttctttaag cgcccgcttg
    14821 agcaggtgat attcacgctg tagttcgcgt cgccgtcggc ccaccgcggc ttgggcttcc
    14881 tgatgctcgt cttccgtcga caccgcggtc agaccgcgcg ccgattgaac gagcataagc
    14941 cccgtcgccc acagcgaagc caccgccccg gccagagcaa tcgacggacc gctcaagctg
    15001 ccaagtgcga tcgaaaagcc gcaaagcccc accgcaaagg tgacgccact accgccatac
    15061 aggagccgag agtcagtcca tgtcatcgag atcgtcgtcg aggcggcgct cataagaagc
    15121 gcgttcttgg tctttgagtg gtgaagaagc ggcggcagaa agccgagcct gctggcccca
    15181 gcgccgcgcc aaaaatccca gtgcgaacag cgccgccgca atgatcacga cgggaaaaat
    15241 ccacgccagg cggttaaagc ccacatcttg tggcaccacc agcgccgatt caccgggata
    15301 tttgtcgaga aagtgacgaa tgatctgctc gcggctgcga ccctcgtcga taagccgagc
    15361 aatgatgccg cgctctttgg cggcaaaacc gcaggtgcac tcggcgagcg tctgccggcc
    15421 acagccgcac atgcagacga tggtcttgcg cagatcacgc tcgaggtcgt tccgcggcgg
    15481 cgtcggaaag ttctggcctt cggtcgactg atgcatcgcc gaatcaccac cgccaccgcc
    15541 cgtcgcccat gccggtccag atgcgcccaa aagcacgagc gctatcaggc tggcgatagc
    15601 ggctttgctt ggccccgacg gtcgatgttc ggtttcgcca tcaaccttgg tcgtcggttc
    15661 gtttcggtcg ggcaaaaacg cgatcgcggt gccaaacatc agcatcacaa agcccagcca
    15721 gacccacagc accagcgggt ttatgatcag cttgagcccc gccaccgatt cgttgccgtc
    15781 gatgccattg aggatcacat acaggtcttc aaacaggctg ttgtgcgtcg cgacaatcgt
    15841 gcgcgggggt tcttcttcgt ggccgcgata ggcccacttg gcgggccgca gctcgaactg
    15901 tgcaccgcca gatttgacca gcagccgcgc ttccgtgacc tctttttgcg gatcggaata
    15961 gctgcggaaa ctcgtaagca gaacctcgta ccgtccgatc ttggtcgact tgccgcgcgc
    16021 gagcgtcacc tcgttttcgg cctggaaggt ctgcccagca aagccaaaaa acatcagcac
    16081 gatgccgacg tggaccagat agccgccgta gcgccgcttg ttgcgcaaaa gcagaccgac
    16141 aagcgccgtc aaaaagtcga gcttggtgcc cgcctgacgc gctcgcgtcc cgacgacaaa
    16201 ttcctgcatc acggtcatca ggaccattgc acacagtcca aagcaaatca ggctcaccgg
    16261 ggcctggatt ttttcgtgca taaacgatga cttgccgcgc gcccacggca gcatgctggc
    16321 gaccaaaatg atcgtcatgg caccgacgac aagcggatag cggaactgct tccacagcat
    16381 cccctgcgac gcacgccgcc acgccatcag cggcccaatc ccggtcaaga gcagcagaat
    16441 caaaccaagc ggcgccatcc agcgattgaa aaacggcggc ccgacggtga ttcggttgcg
    16501 cgtcaccgcc tcggacagcg ttggaaacag cgtcgccacc aggatgaaga acgcgcaggc
    16561 aatcagcacc cagttgttga gcagaaacgc aaactcacgc gagagcagcg attccaagtc
    16621 ggcgcgggaa cgcaaaagcg gcagccggta gaccagatag ccaaacgaaa acaccgcagc
    16681 aaagccgatg aacgccaaaa agcgcgccgc cagctcgggg tcgttgccga atgcatgcac
    16741 cgactggacg atgcccgatc gggtcataaa cgtcccaacc agcgtcaaaa ggaacgtcag
    16801 cacgacgagc acgacattcc agaccttcat catgccgcgt cgctcttgga tcatcagcga
    16861 gtgcagaaac gccgtcgctg taaaccaggg caaaatgccg gcattttcga cgggatccca
    16921 gccccagtaa ccgccccagc caaggacgtg ataggcccac aatgagccga ggatgagccc
    16981 ctgcgacaga aagtaccagc agatgagcat ccagcgccgc accgactgaa gccaagtgtc
    17041 gtcaagattg ccggtgataa gcgccgccat acaaaacgca aagggcacgc tcgccgagac
    17101 gtagccgacg tacagagacg gcggatgcgt cgccatgtag aagtcttgca gaagcggatt
    17161 gagccctttg cccgccgccg gaacttcgtt taggaaggtg tcaaatggcc gcttgtaaaa
    17221 caccaccagc gacacaaaaa acgcctgaat tcccatcaga atcgccgtga cccacggcat
    17281 cagctcgcgg tgccgttcgc ggtttcgggc aatcgccacc gtcgaaaaaa tcgacagaag
    17341 ccacgcccaa aacatcatcg acccgtcgag accgccccag tacgacgtca gcttgtaaaa
    17401 ccacggcatc gccgcgtcgg agttctggtg gacatacttg agcgaaaagt cgttcgacag
    17461 caccgcgaag aaaatcagcg ccgaggccag gctcaaaagc gccgtgcagg catagccggc
    17521 gtagattgcc gaacgcacaa gctgcggcga ctgacgacgc gcccccgcga ccgccgcgcc
    17581 tcccgcatac gtcgtcaaaa ccagcgtaca gagcagcacg aagctgccaa aaagagccac
    17641 ccagttggcc tgtgccatgg cttagttgcc cttgcctgcc gagtcgcggc tacagcgctg
    17701 cacagccgtc ggatgactcc cgcccgcttg ctgatatttc gacggacact tggccatcac
    17761 ttcgtttgcg gtgaactgct gcccgtggag ctggcctttg aggacgactt cggccccgtc
    17821 tttgaacgtg tcgggtacga cgccagcaaa gtaggcttca gcgacggcgc cacagttttc
    17881 gatttgaaac ttgtattcca gcttctgcac gtcgtggttc atgcgttttt tgatcgaacc
    17941 agggacgacg tagccgtgga gctgaagtcg tttgttttgc caactgccgc tctctttggc
    18001 gacctcgtcg acgtgtttgt agtactcaaa gcccgaggaa tccgagagca aaagaccgat
    18061 tcccccgacg atgatgccga ccgacagcac gatgaggagc ccgcggctgc gaccactcgc
    18121 tttgggcgga cccacggaag gaccactcgg gagcgcggat gatgtcttta ccgtcatgac
    18181 gtttcgacct tttaagttca catattttgg cagcttccgc gcctacaaaa tcaccgtggc
    18241 gcgtcagccg ccgatagaaa ccatgtgttt gcgcggtccg ccgcagcccg actctgaaaa
    18301 cacatgccca gcccgctttt cagaaagtcc gccgcgcgca acttggacca gccgctgcat
    18361 caaatcgtcc gcagtcagcc ccgagcgtaa cacgctgcgg aggtctatcg catcgtcgta
    18421 tgccagacag ccgtgaagct gtcccgtcgc tgacaaacga atgcgattgc aggtgtcaca
    18481 aaacggctcg gtcactgccg aaatcatccc cacttgacgc agtgcgccat gatattcgac
    18541 cgcggcatag cgtgcgggcc caacgcccgg cagaagcggc tcgccttctg cgcggtacag
    18601 cggaccaaac gcgcgggtca aaatctgtcg cagctcagag gcgggcaaaa atgttcccgg
    18661 ataaaacagt gtgccgtcgg acatcggcat cagctcgata aaccgcggaa tgaagtcgcg
    18721 atcccagcag taggcacaca gcgctgcgaa ctcgtcgtcg ttgaagccgc gcatcgccac
    18781 gcagttgacc ttggtgtgga caaaaccaac ggcacgcgcc ttgtcgagtc cagccaagac
    18841 ctgggccaaa ttgccgcgcc gcgtcacttg aagaaagcga tcttcacgca gtgtgtcaac
    18901 gctgacattg agtcggtgaa gcccggcatc cttgagcggc gctgcaagct cgacgagccg
    18961 ttcaccattg gtcgacagcg acagatcgtc gagccccagc cgagagagcc gctgaaccag
    19021 atcgatcaag tccttgcgca ccgtcggttc gccgccagtc agcctgactt tgcgcacgcc
    19081 gacgcgcatc agggcttcga ccgcgtgctc gatttcctcg aatgacagca cgtcggcacg
    19141 cggcgcaaga tcgatgcctt cgtcgggcat gcagtaggta cagcgatagt tacagcggtc
    19201 ggtcagcgaa atccgcaggt agcttatgct gcgcgaaagt ccgtcgacaa gtgggccagc
    19261 tgcagttcca ggtccggtcc gcactgccgg ttttccgtcg acaatcggaa gatgaagcga
    19321 actacttccc gatctgctcg tcgtcccgca agaactgcca tctctcatcg tcgcgcctcg
    19381 aactttagat gcccaagctc gggcccgatg agcttttcca tcgccaaacg caccgcatgc
    19441 ggtgaacccg gcaccgcaaa aatcaccagc ccacgacaca gaccggcaac cgcgcgcgac
    19501 agcatcgccg gtgaaccaat ttcggcatac gacagatagc gaaacagctc cccaaagcca
    19561 tcgagcctct tttcgagcag cgacgacacg gcttcaaacg tgctgtcgcg gcgagaaatc
    19621 ccggtgcccc cggtgctgat gatgacgtcg gcggactgct cgtcggcaat cttgcccatc
    19681 acggcctgaa ccagcgtcgg ttcgtcacgc acaatcgctt ttccgacgac cgtgtgccca
    19741 aacgactcga caagctcgcg gcagacccgg cccccttcgt cgttttcctc ggtgcgggta
    19801 tccgaaacgg tcaggacata gacgcgaacg tgacggggag cgtgggcttt gtgctgctgt
    19861 gcgggcgtca tgggcggagc atcctagcaa attataacgt tacgtcagct tttgcagccg
    19921 gcgtattctt cttgcatctt cgtcgagtcg ctttggcttg ccaaagacaa aagtccaccg
    19981 ctcaggctgt gaacctgagt cggcggcttt gctgatgtcg aggggaggaa tcacgccgca
    20041 agagatggcg gcgtcgatgc tgcgggaagg actagccgat ggcgatgccg aggagcgcgc
    20101 gcagctcgaa gtaggtcggc gagatggagt acagcaccag ctctttgacc ttgtccttcg
    20161 gagccatgcc gccaactgcc gtcggttcct gaccgatgta gcgcgccgcg acactgaggt
    20221 cgttgcagac cacaaggccc gctcggtgcg acgtcagatc gaccgcctgc gcccacttcg
    20281 ccatgtcgag ctgcgacttg ttggcgatgt acttctgcac caccaagccc aggtgctctc
    20341 gcgccgcgaa cgggacgaac ggacccatcg cgcccgagaa ctggtcgacg aactgcgcgt
    20401 gctgcggcgg caccgggaag tccggacgag ccaggcgcac cgccgagagc agcgcgacag
    20461 ctagctcagt gttcgactgc aagatggccc gcaggtagtg ctcgggacgc atcttggtga
    20521 ggaacaccgc actcgggaaa gcgacgtcct tgtcaccgcg gccagccaac atcccttgtc
    20581 cgacgacaat cgacgggatg aagaggttct tctccttgca gttggcgacg acgagatcgc
    20641 cgggctgctc ggtgcggaag aatatctcga cgttcggcgg cacattgagc acctgcatga
    20701 cgtaggtgaa cacgcgccca aacatcgtct ggtcactcga cagatcgcgc ttttccttgc
    20761 gcttgagtcc gtactgcttg tgctctgcgg cgcgcaggtt gccaaccgcc gagtacaccg
    20821 tcgcaaagat ggccccgatg aagcggtcct catccttgtg gtagatgtac ttggcccaca
    20881 tctcgtcggt gagcttgttg cgcgccttcg gcagaccctt cttgtactgc tcgtagaact
    20941 gctgctcgtc ggcgtccgct cgctgcagat aggtcagcgc cgaacacatg caccacgcct
    21001 tgtcgaactg gcgtgaatcc atgtagatgc gacgcagagc cttgtacgcc tcgacgcggt
    21061 acggatcctt gtcgatgagg aacatctgct cgttgacggc cttgtcgagg taatcggggc
    21121 caccgacgat atagagctca ccgaggatct cgcgacgctt gaggttttcc ggctcgaggc
    21181 caactgccac ctcgaacgtc tggattgccg ccgggatgtc cttctgacgc gagcgataga
    21241 tttcgccgag tgcgtgccac agcgcaacgg tgacctgctc ctgcccggtc ttgggcatgc
    21301 gcttgatcat cttgcggtag ttgcgctcct gcgccttcca gtccttgcgc gtcgtgcaga
    21361 tcttgtcgat ggcttcaaac ggcttcagga actcctggaa ccgcgccgaa ggaatcagct
    21421 ccggcttgct gaagtacgag tcgagcgcca ggttgaactg gtcgatcgct tcatccgtcg
    21481 acttgagcgc gtcgcgatag ataacgcccg cggtgtagta gaacttgccg cgacggagca
    21541 ggtcgtgctc aagctgcgcg agccgtccga ggatctcgat cgcttttttc cactgctcgg
    21601 tcttggtgta aagctccagg accttgtgca gcgtgacgtg gctgtccggc ttgatctcga
    21661 tcgcatcgag gtagctctgg atggccttcg ggtactgctg gagcttgtcc gcgtacattt
    21721 cggccatttc gcagtcgatc ttgaacttct cctcggcttc cgagacgggc aaaagtgccc
    21781 gcttggcgct gatgaccgct tcccagtcgt tgttggcaag ctgcatctcg atgacggcga
    21841 ggagcgtcgg cctgtggttc gggtcgagct cgagcgcctt ctcgaacatg ttgagcgcct
    21901 tcttcttttc cgtctggcgc agcttgatga tgcccaggcg gtagaagata tcgacgactt
    21961 cacggtcaga ctgcgcctcg cggtgatgca cgagcaccgt ctggtacagc ttgaacgcac
    22021 cttcccaatc ctcgaggcga tacagaaggt cggcccggcc catcagcgtc tggagtgacg
    22081 tgctgtccag gtcataggcc atcttgaagt acttgagggc cttgtcccgg ttgccgagag
    22141 cgtccgccgt cttcgcgacg cggaagtaca ggttgttcat ctccttcgca tcgcgcttat
    22201 cggccttgcg gaccagcgcg tcgaggatcg gctcaagttc tttccaacgt ccctcgcgga
    22261 agtacagctc agccagcggc tcggcggcac cgacgtgctc tgggtcgagc tgcatgacac
    22321 gggcgaggaa ctccgcaccc ttcttttcgt cgtcgagacg ctcgcgcagg atcgtgccga
    22381 tctcgaagag cagcttgatc ttttcgagcg ggttcggcgt ctgctcttcg gcgcggatca
    22441 tgagctgcga cgccttcagc cagtcgccac ggcccttgta cagcgcgatg agcttgtgca
    22501 tcgccggaac gtagttcggg tcgacgcgca gcgcttcgag gaagcggcct tccgcggttt
    22561 cctgatcctg gagcttgtct tcgtacagcc caccgatccg cgtgtgcagc tcgacttgct
    22621 gacgacgatc ctgaaccagc gacaccaagt gcgacagggt gtcgatggcg cggctccagt
    22681 cttcgacgcg ctcgtacagt cggccaaggg caatcagcgc accctggtgg ttcgggtcga
    22741 tggcgagaat ctcgctgtac gactcgatgg cgcggtcgtt gtccttgatt tcgacttcgt
    22801 agacctcacc catcgcagcg tagagcgaca tccgctcggc gccgtccacc gacacactga
    22861 tgtgccgacg gtaggtgtcg accagctctg gccactgctt caggatgcga tacagccgct
    22921 cgagctgacg gtacgacggg aagtgacgct cgctgaacgt gagtgccttc tcgtgcgctt
    22981 cggcagcctt gtcgaccttc ttgaacaggt tttcccaagc gacggcgatc ttttcgtaca
    23041 ggctgatccg ctcgtcgtcc gatccaacca cgtcaagctg cgtctccagg atttgcaggt
    23101 acggctcggc ctgctggacc ttggcataca gctcctcgag ggcccgcatc gcgcgcaggt
    23161 tgttggcgtc gatgtcgagg atctcgcggt ggctgatgat cgccttgttc gggtccgaca
    23221 gccgctgatc gtacagctcg ccgatccgcc gcttgagcat gatgaccttg tcgggatcat
    23281 cggtgttctt ggccttgcga ccgagaatgt cgatgaggtt tatccacgac tggcccgcgg
    23341 tgtacagccg ctcgagggaa tcgagcgctg gcatgcaggt cgggtcggca ctgatcgccc
    23401 gacgcagcgc gccgattgct tgctcggcgt tctgcatctg gttttcatag atgcccgcca
    23461 ggtcgagcag gatcgcgact ttcttctcgg catcgtcttc aatcgacgag tggtgctcga
    23521 gcgtcgccac cagttcgttc cactgctgcg tctttttgta cgcttcagcg aggttggtga
    23581 gcgcttcctt gtggttcttg tcgatctgga gcgcctgctg ctgcgacgca atcgcgtagt
    23641 cgatgcgatt caggtgctcg gcgtaccagc gaccgatctt gacccacagg tctgcgcgcg
    23701 ccttcgggtc ggcgatctgc gtgacgctct gcgtgtactc accaagcagg tctccccact
    23761 tgttgatgat cgaggccaga cgttccagct cacgcgccgt cgagtcaacc gagtagtcct
    23821 cacggaacgc cgcttgaaga atcacgaacg cgctgtcgcg atcgttcatg ttgtcttcgt
    23881 aaatgcgggc aacctgctgc aaaagctcga tgcgatgcgt cggctggttg tccgggaact
    23941 ccacgcgggc gagcagaagg tccgccagct ggttccagtt tttgcgggtc cggaagatgc
    24001 cctcaagctg aaccgaggct tccaggttgg tcttgtcgat ggcctgcaca cgctcgtagg
    24061 cgtcggcagc cgcggccaaa tccgagactt tttcttccca gatgtgaccg atcttcagaa
    24121 gctcagcgat cttctggctg tctttttcga gcagcttgac gcgcttctcg atgacgttga
    24181 ccgcgtcttc ccactggctt tcggcgccgt acagaacctc aagcgcatcc agcgcacgga
    24241 agtcacgctt gttgatctcg aagaccttga gccacgcctt gatcgcatcc tggggccgca
    24301 tgatgcggtc cgagtacagc ttgccaagct cggcccacgc ttcgcggatc tcttcgatgt
    24361 tgccaccgac gagcgtcagc gaatcgatca agcggtgcag gatctcggcc agatcgtcgg
    24421 gctgatcgag cttgcgatag agcgccgcga cggcccgcag cgcatcaatg tcgcgcgggt
    24481 cga
//
LOCUS       DAORGN010000057        23912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_831_length_23912_cov_5.167833, whole genome shotgun sequence.
ACCESSION   DAORGN010000057 DAORGN010000000
VERSION     DAORGN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23912
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_23912_cov_5.167833"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1799
                     /locus_tag="PKL17_08535"
     CDS             114..1799
                     /locus_tag="PKL17_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNK44814.1"
                     /translation="MMVKDPEAAGGERAKVLDFGIAKIAEEPNLKTQAGSVLGTPAYM
                     APEQCKGASGVTSKSDVYALGVMMYEMLCGQTPFQGEGLGDIMVKHMTEEPPPLQRRQ
                     PAVSEPVAALVSRMLAKSPDARPSMRQVIEETERLGAMKTGVMSAFSETPASAVAPNG
                     TLVLADGQPATPQTLALGDKAALHGQTAITSSPGGSSRGKLLVMLGLAASVLLVGAVV
                     LLRSRKPPPPAPLAEHPQGKPDRKIPPADPSPEPRRKPSVPVPIGMVFVPGGRFEMGS
                     TDGEVDMAFKLCQKRGSGCRRDVFERETPRHAVVVRDLFVDQYEVQNEQFVSFLGTQK
                     GLKLDKKKFVKGAKGILIEVGAESGIVAASKKLTFTLREGFAKKPVAMVSWSAAKLYC
                     EAQGKRLLTEAEWELAARGLGRRTYPWGDNQPKCGEVAFGRDPALPNSARCRNLESTP
                     DPVGKSTQDRSPDGVYDLGGNVTEWVADAFLEHYEPCQGACKEPQISDESIASAGKKK
                     NKKGPAPLHVARGGNFNQSLDGCRSAGRNRFPEDTLLPTLGFRCAKAAAPSGS"
     gene            1924..2217
                     /locus_tag="PKL17_08540"
     CDS             1924..2217
                     /locus_tag="PKL17_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44815.1"
                     /translation="MPRSMTTATLFFAALFVGVCFGSVSSAKTAHTESQPSGPSADLR
                     SEAPPPHDPTKKNPGEPCKDAKECQPHHQCVKVGDKNVCQAPPRPQLPPGAVT"
     gene            2283..3335
                     /locus_tag="PKL17_08545"
     CDS             2283..3335
                     /locus_tag="PKL17_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44816.1"
                     /translation="MRSSSAKTASVVQSAWLQDARFDLVFVVGIALLAAVMSAVTVVS
                     PELFLAMLTVHTWLFGYEHLAATYTRLFGRPEDRERFFRLTFWVPPLVLLALCAVSRT
                     SGLIGIYVVYFFGQLHHTVRQSFGIAQQYRQRAGGLPWDPRWQSQLTLWSVPIWGLLH
                     RCVQRPVEFLFQPFWLPPVPRALETCAAIASAGLWLYWFYTRVAAYRRGELALGHTLY
                     MLSHLFVYLGGYILIDDLCSGWLLVNVWHNVQYIVFVWHYNRRRFASGIDPQSRVLSW
                     LSQPGWVRTALYGLSSVLFALPLYYLLPRLGLTLDKLVAQTAVPSAILIGLSLTFHHY
                     IVDAFVWKRRNDPTAI"
     gene            3416..6424
                     /locus_tag="PKL17_08550"
     CDS             3416..6424
                     /locus_tag="PKL17_08550"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNK44817.1"
                     /translation="MKKRMAAWGTQMMGTRNGWAAWSLVLGLVWGCSTNGTDGTPSGD
                     VVDAAVPGIPPLINEFVTRNQTGLTDEDGSTSDWIELVSQDTVPFDLGGYHLTDSKAS
                     PKRWTFPEGTVIQPGAFLVVFASGKNRIVAGKPLHTNFSLSSGSPQGSCSGQSGGYLA
                     LTDRDGVPVGPVFDPYPAQSADVAYGLRSPVPTADRSYFQKPTPAAPNNLGSAPADRV
                     VIEPSSRTFAVITPLQVTLSTASPTAVIRYTTNRARPIAVPGSVGTFAADAMTDTCTQ
                     TAHGLQNGDPVRVSGPAPLTSGNTYFAVVLSPDTFKLAVEPGGPPIDLTASGTFEVRR
                     DAANGTAATSDIITTTYPHTFFAGDSVTVSTSGTLPAPLTAGTTYYVDVLSATTLRLS
                     TSPTLVPAVDITTTGSGSLTVSRTPSPTYSKPLSVSVTTRVRARAFETGRPDGPLVGA
                     MYFALDAAAQTANSNLPLVLTHSFNTAILNNNVPIDSYIMFFEPKAPDNLAKMTNLPD
                     LVAPCTLERHGSSTGGEAKFSMVVELQDENGIDQNCSPFGMPAESDWMLNAPYRYDRS
                     MMHNDLLYRLSNNAGRWAVHTKLVEHFHNEQSAPDTIEGSFTGVDYFGVYSFMEKIKR
                     GPSRVNVEGLSIGDSTTPTIAGGYIFKADRLDPGEVGIVPLSGQSFGGMGGNVMPWVY
                     PRELSPDPFKVVNLAQSNWLRGHLGEAWSVLGSANYADPVNGYAKYWDVAALIDNHIL
                     NAASKNADAFRLSAFWHKPRLGKITAGPVWDYDRAMGSTDGRDFDYGTWTGVAGFFNY
                     AWYVQMFADPNFRQAWIDRLHELRQGPMSTQAIHAHIDEFAGLLNPGDGAGTPAKRSA
                     VRWPVSAPRTTANNTAITNNLFDGTYPGEVAWLKYWWEKRLAFMDGELTRPAVANIPS
                     GKVAIGTTITLSSPSQSLPGVKLYYTTDGSDPRPPAPGPVLSAKAIEYTGPITISGPT
                     QLVVRTYNPTPATVAVTTNWSAKTVLDYTQ"
     gene            complement(6457..6948)
                     /locus_tag="PKL17_08555"
     CDS             complement(6457..6948)
                     /locus_tag="PKL17_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNK44818.1"
                     /translation="MQPCPCGSGNVLAGCCGIYHANPGTAPTAEALMRSRYSAYVVRN
                     SDYLLLTWAKAQRPRKLDLAGDQTEWLGLHIERTEAGRETDDTGRVAFVARFRSQGVS
                     QALHEDSQFVRENGSWVYVDGTSELRRTNAGTVKHAAQTPSRNDPCTCGSGLKWKKCC
                     GRS"
     gene            complement(6948..7331)
                     /locus_tag="PKL17_08560"
     CDS             complement(6948..7331)
                     /locus_tag="PKL17_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNK44819.1"
                     /translation="MQLVGTLLVVLVAVEHLWFLILEMFLFRTPVGLETFQMSQQKAD
                     ACATLAQNQGLYNGFLAAGLLTALLTKSALLRRFSLACVIVAGIYGCYSLGSQTVLLF
                     QSGPALLALLITEAAQKRQSETGAR"
     gene            complement(7528..9321)
                     /locus_tag="PKL17_08565"
     CDS             complement(7528..9321)
                     /locus_tag="PKL17_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44820.1"
                     /translation="MIPWLRHFRLPIQAGMSRLTALVCGIALHGCTPPSQPIVMVTVN
                     GITSDLVTETVQTTLNGQKSVNSLDTVTRRLDHIELRLAETAVGTLGIAISAQGSDGC
                     TARAGKTSVDITGPGNYSASVDLADEQGCTLVVRKEGDGAARVTLSDGTAWTFSPPEP
                     PEAMCPIDLIVGTSMQKAFPFGTKLELKSDTAWDIAPDSYIAGVAGCTQSAAGCGVTI
                     GADKVQVTVRAGRNTVCSNDQVCWEHPRPQGLHLRRIKGSNNDDIWASGDGAILHWEG
                     TYWSSPRRARLPNMFSGIVSADLGSAVFVGNSGTVSRLISDTWSCVESMGSQDLRAAW
                     GQSATDFWVVGGQGTLAHWNGTAWTTSPIAGSVELWAISGTSASNIWAVGDGSTVLHY
                     DGTSWNKVSLGASIPTTLSLRGVYANPEGEVWIVGDGGTSLSVSGGKTTTWATGTTAK
                     LNDIFAIPGQAKWAVGDSGTLLRHDGKSWSPVDSGTKEALNSLWGAENTSIWAVGNYG
                     TLLRFNGVFWTVASSARKTQNLYALGSIPAASSTGNPIFVVGDQGTVMSTSGADWLLD
                     PNTNGITTRTLRAVSTTPTNLWFAGDAGTIV"
     gene            complement(9349..9990)
                     /locus_tag="PKL17_08570"
     CDS             complement(9349..9990)
                     /locus_tag="PKL17_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNK44821.1"
                     /translation="MLAVRKVTQHAQALYLAAGLAFCLLPGCKHTRPNGSQQTRGLGA
                     ATDDLPASGPLTVSLTAMLSEGSTERPLLASESLHAGDHLYFLVRASQPAYLYVVLYG
                     PSGTPSILFPRKGEPARIAARCPVSIPAQGSFYLRNPAGNHDLRVIASTEPLEQADRK
                     LCDQLQLPCETPIPDAVKTCPKERARALFPSMQVATATPLGVASLRLVLRQDP"
     gene            complement(10034..11353)
                     /locus_tag="PKL17_08575"
     CDS             complement(10034..11353)
                     /locus_tag="PKL17_08575"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNK44822.1"
                     /translation="MQKSVRRCRFLLAGTAAQLLAAQPAWAQSPTARSTENPAAAAGE
                     LRSTTPRPHIELLSSTVNKDGSITVEAQVEDPAGGEVELAILQDGEPVKALTVAQGRD
                     LATASRYTLQIRPPHKDGVLSIVARSGSTSSQPLTLRLRKGVPERRPALYVLAIGVGR
                     YQSTDIPSLRFAAKDAKDLATTLQKQRDMLYREISTRVLTDGQAIRSQVMEGLHWLTT
                     QVGKDDTAVVFLAGHGTNDPDGMYYFLPTDAQADRATMLSGSELQDALRQIPGRVVLM
                     LDTCHSGNVLGKRSLNRFINDLTSENRIVVFAASTGDQVARESSDWNNGVFTKALVEG
                     LRGVADYAEDSKITVSELETWTSVRVSQLTAGAQTPTLAKPNAVPDYTVAALPEQGVL
                     PNPKQAVRRRLLWGAGGVSLGLAVGIGLAVGLSLTISGSQNVLEPRF"
     gene            11586..13181
                     /locus_tag="PKL17_08580"
     CDS             11586..13181
                     /locus_tag="PKL17_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNK44823.1"
                     /translation="MPSSLTYPGVYIEEIPSGIRTVTGVATSVTAFVGRAQRGPVGEP
                     VVLSSFRDYERNFGGLWLDSTMSFAVYDFFKNGGSQAVIVRAFKKPTTTATDGSTTSA
                     SGVATFTADTLGLSAKSPGTWAANLLVSVDYTDRTSDVSAEIAKNLGIATSDLFNVAI
                     TYFAASGAVTERFVNVNLKDNVRRVDRVLEQKSLFVRVTAGSDGNPTLPTAVPAASTA
                     NATTGEVGVRANTGTALDSLPLTSTEYDFSVLDKTDTVNLICVPPDTRGGDTANSVYS
                     NALTYAVKRRAILIVDPPAAWSSKAAQDIKTSDLGLTGPAARNAVVYYPRIRKPNPLR
                     SDLVEEFAPSGLCAGIISRTDTQRGIWKSPAGIDAALYGVDSVLPKLSDSDSGTLNPM
                     GVNCIRSFPASGQVIWGARTLRGADALGDEYKYLSVRRLALYLEESIYRGTQWAVFEP
                     NDEPLWAQIRLNIGTFMQNLFRQGAFQGSSPRDAYFVKCDKETTTAADIQAGIVNIVI
                     GFAPLKPAEFVVIKFQQAAGQSS"
     gene            13194..13721
                     /locus_tag="PKL17_08585"
     CDS             13194..13721
                     /locus_tag="PKL17_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK44824.1"
                     /translation="MPQFTVNTHRFEPYQNYRFRIKWDGRYVAGVTKVSSLRRSTEVV
                     KFREGGDPSSSRKLPGRTEYEAITLERGVTHDAEFERWANKVSTYGAGLGAERSLKDF
                     RKDVIIELLNEAGQIAISYRVYRCWVSEYQAMPDLDASGNAVAISHIKLEHEGWERDT
                     AQTEPVEPTFQLPST"
     gene            13746..14477
                     /locus_tag="PKL17_08590"
     CDS             13746..14477
                     /locus_tag="PKL17_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44825.1"
                     /translation="MMASEAEILRGFDLCDGHSDVDRAVLMLLWGYPERRLPELLALP
                     IGARDSLLLALCERTFGDRVSGSALCPVCQQRSTFFLRASDLRLPPQQQELPLVLRHG
                     DWELHYRLPTSYALRGAAACPDVESAADSILRYCLLSARHKETPVSCTDLPIDICQRI
                     GQALALADPQSEVLLSLRCAHCGHNWQSLFDIARFLWTELSHKAQRVFDEVHTLASKY
                     GWSEQEILHMSPVRRRQYLQRCGHE"
     gene            14470..15708
                     /locus_tag="PKL17_08595"
     CDS             14470..15708
                     /locus_tag="PKL17_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44826.1"
                     /translation="MSNFFQHLALRALGQVRKSDAQPVVRSRFEPDSQRSRNLLDEVG
                     QPDPQEHENVPDLGHTDQTSLANIRPRVTAGQAGFTVSASDRTAEPSLRASAQVVPHG
                     DTPNSHDVAPGPEDGPQSLELSEPSLAGATPSRHDVMPRGSKEPGPDPFSVDPLAEVP
                     DPGVSVPSSKDKPRTTTGKSADLQDADFGDAQDGRSTNLPASDPTSDSQPSGTVGKAS
                     PDSIRQRSNNADAAGTTSEGQPRQLSRVSPLSAEPDRSSANSERVPSVEETESDSGSG
                     ASGDRAARTVADPRVSVAGPRLPASHASRAGTRNTGHSETEEVQGTDFASAPSSALAQ
                     TDVSHGAGQGISLQTAKPKSESAGPSQSVSAPQVHIRIGRIEVRAPAPKPAMVQAPVP
                     PPRTPSVSLSDYLKQTRGRG"
     gene            15717..16322
                     /locus_tag="PKL17_08600"
     CDS             15717..16322
                     /locus_tag="PKL17_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNK44827.1"
                     /translation="MSVAAVTAALRNRLTVVSSPLPGETTTDLSDTQVTTLPPDKAGL
                     QEDHNQINLFLYQIQPSPSGRNLDSRGQAGRKPALALELLYLVTFYGRNANEILTQRL
                     LGRAMSLLHSAPALAPAELAAALPGSDLDKATDLVRLVPHLMPNEEMVRLWGTFQVKY
                     RLSVAYRASIILIDHEYPDTILPAPTERRITLGPIPDGGSL"
     gene            16319..18334
                     /locus_tag="PKL17_08605"
     CDS             16319..18334
                     /locus_tag="PKL17_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44828.1"
                     /translation="MSDGGPSSPVPSVHDRWLSGNQQYLLLSLARVRKALLDYLLRSQ
                     PDAVVNAVPEPLPQAVDADSLAASMPAPPALLSLCAAFELSAFERDLLLLCVGVELEP
                     AFRRLCQKAGGTAQPTFQLALSALAQPHLSAVSPSGPLRRFGLVEALSSETLTASPLR
                     IDPRILSHLTGLSLVDERLLAVGRLLSPPDEPLGTALSSLAQQIVRLWSVDTEDAPPA
                     VSLFGADGGERLALAQHACQPLGLLLFEVRCADLPHSPAERAQLAKLWAREQSLLHIA
                     LLLSVADGDSPETVRQAMLWIAQLPGAVLCSSPTPLRDAQKRMVGIEVPRMLPDERTE
                     LLTELFEELDPQLASMRGPGGESLLSELSMQFQIGREAVVAACQRASGMSLPLPQALW
                     QSFRMQARPHLDELARRVEPKVTWDDLVLADEVKQKLRELLAHQRYQVTVLRSWGMAG
                     TAQRGLGVSALLCGQSGTGKTFAAEAIAHELQLDLYHIDLSQIVSKYIGETEKNLRRV
                     FDAAEEGGAVLLFDEADALFGKRSEVKDSHDRYANIEVSYLLQRMESYQGITLLTSNL
                     RSALDSAFLRRLRFIIPFPFPDANQRAELWRRAFAKQTPTRGLVFEKLAQLSVTGGQI
                     RNIALCAAFLAASQGQPVGMRHILDAARSEYQKSERPLNENEVRGWL"
     gene            18325..18678
                     /locus_tag="PKL17_08610"
     CDS             18325..18678
                     /locus_tag="PKL17_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44829.1"
                     /translation="MAVTHQVFLHIDEIVLDGVEGTGDFQAELERALLAQLAAPGLAA
                     LMVGGGDFRELSLGQLQLGELRIGMSTLGSALGSALGSALVSRLPASPPQVGSTGVAL
                     PGLTAGPNRGVAGKE"
     gene            18681..19388
                     /locus_tag="PKL17_08615"
     CDS             18681..19388
                     /locus_tag="PKL17_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44830.1"
                     /translation="MGIADKVSQTALSYAEQAISSIPPLRKGALIHGPMLIPFQYNPE
                     KLTRTLTASSMGTSGPDLLRLKGPPGEKIALEILLDAADQDNRQSGLQLADGIYPSLA
                     ALEMIIYPSSARVLANAALSQMGIIELIPPLPVMTVLVWGYRRVVPVRIESFTVTEEL
                     FDSALNPLRAHVGLGLQVLTYEDLGLLSAGGMLSMAGHVQREVLAASAAIRSTIGAAS
                     VTGLDMNVVSKAIKGIL"
     gene            19411..19695
                     /locus_tag="PKL17_08620"
     CDS             19411..19695
                     /locus_tag="PKL17_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44831.1"
                     /translation="MLDPNSRYYNIETAQLEQGGRSVRYLTRRMLPALAAQTTLEEYQ
                     AAGSERLDLLASMGYGDPLQAYRLIDANPVLHPSELLVARRRIRIALSRL"
     gene            19745..20860
                     /locus_tag="PKL17_08625"
     CDS             19745..20860
                     /locus_tag="PKL17_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44832.1"
                     /translation="MQANQLGVRLVLWAGIVPAPVPQSVLEAIEKLEIERSGDDVGGF
                     QLTLRAEKTVGVEFDLLRSPQLLVGSRVVIGVLMGVTPEWLLDGVITHRELLPSDKPG
                     SSRLCLTGRDLSAVMDRMDVVMSYPGCSDSVIAAAILVRYAPYGVVPQVSPTVDVPLP
                     TERVPLQHETDLRCLRRLARDNNYVFFIEPLGFGASRAYFGPDLRFALPLSPLRVDMG
                     ARSNVDALTFKQDGDRAEFVEGRAYPPGFPVAVPIVGLPLPPVPPLAAIPTLPLHYRF
                     DGSLTARSIPRALLAATGAQARANHRAVECEGTVDTVRYGSVMQPRRLVGVQGAGYSF
                     DGYYSVSKVRHELSRHKYTQKFSLVREGVGSLTPAVM"
     gene            20860..21459
                     /locus_tag="PKL17_08630"
     CDS             20860..21459
                     /locus_tag="PKL17_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNK44833.1"
                     /translation="MADRPTFYGKYQGVVVNVLDPERRGRILVQVPAVYKDSVSGWAE
                     PCLPLVGIRSGLLALPQVGAKVWIEFEQGDPERPIWCGGFWAPGTAPSAIAPLQTVLQ
                     TTGGNVILIDDTPGVGGITLKTIAGDTFKLSPATGIALQTFTGEKIEVSPVGITIQSA
                     AGHSIMINAAGICLSTSTGATVQLTGNIVDINAGALEVI"
     gene            21459..21785
                     /locus_tag="PKL17_08635"
     CDS             21459..21785
                     /locus_tag="PKL17_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44834.1"
                     /translation="MGAPLLHAGNQITCVHGGQGEVIFTNFRVSTRGNYFMTVGDTVS
                     FAGCPGTPPPAQLVCVIAQYAMPATRVFICGQPALLQSSIATATPTGTPAVTAPAPPA
                     SFVGGM"
     gene            21787..22194
                     /locus_tag="PKL17_08640"
     CDS             21787..22194
                     /locus_tag="PKL17_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNK44835.1"
                     /translation="MAMRFPYQLSSRGTTATASASEDAYIRDLMEQVLLTSPGERVNR
                     PDFGSGLLGMVFAPGGDVLQAALQANVQATLQRWLQDLVLIQAVSVDVVDSAAYITIQ
                     YVVQRTQQTQVARFSTEPSSASFSASVSAGGAS"
     gene            22191..>23912
                     /locus_tag="PKL17_08645"
     CDS             22191..>23912
                     /locus_tag="PKL17_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNK44836.1"
                     /translation="MSQDASQDTTRTSTATTQDTPDIDYLSRDFASFRRQMLDRLATI
                     SPGTASGHPADMGTVLVEVLSQAADELSMFQDAVATEAYLATARLRTSVRRHARLLDY
                     RLHDGCNARVWVTVAVSDAADGQVLPQGTRLLSRVEGQPTQLGEDDIRSLRSRGALVF
                     ETMQSLLLRSAHSVMALDVQAGPVLAGAQSAQLVQSQTTKLALRVGDVVIFEQLAVEA
                     GAAAALDRRHAVRLTSVTDTSTSTAVGALKIGWSLDDALPFALDPRRTQVLGNVVLAD
                     HGYTLPDAELIQFENSRARPQLLQGPLTWQTMVLSGGQALRFDPNGAAASVVPTEILP
                     STMISPVISLVDEVGQPWQARRDLLSSAALARDFVVETEDDGQTYLRFGDGVYGRLAQ
                     GPLFARYRIGNGSTGNIGADSLSHIGSLVPGIRSVRNPLPARGGKDPEPLDRVRNLAP
                     RQFQVAERAVTTEDYAQIALRFPTVREVRAMRRYTGSFFTTRLFVVRFGGLPVDQAFC
                     DSLLDFLSHYRMAGHVLRIDGPVKIPLDIRLTVGVKSGHFRGDVVAALHAALGPGTSS
                     KPGFFHPD"
BASE COUNT         4391 a         7206 c         7452 g         4863 t
ORIGIN      
        1 gtttcgagga gaacagagcc acatagcgtt ttcccgcgca cttcccagaa gcgatcaaga
       61 acattcattt ctagacgatt ttttagtgat ctaacactca gccagacaac gtcatcatgg
      121 tgaaagatcc ggaggcggcg gggggcgagc gggcgaaggt tctcgacttt gggattgcga
      181 agattgcgga agagccgaac ctgaaaaccc aggcgggttc ggtgctggga acgccggcat
      241 acatggcgcc agagcagtgc aaaggcgcgt cgggtgtgac gtcgaaaagt gatgtctatg
      301 cactcggggt catgatgtac gaaatgctgt gtggccagac tccgttccag ggcgaaggac
      361 tgggggacat catggtcaaa cacatgacgg aagaaccccc gccgctgcaa aggcgtcagc
      421 ctgccgtcag tgaacctgtc gctgcgctcg tttctcggat gctggccaaa agtccggacg
      481 cgcggccttc gatgcggcag gtgatcgagg aaaccgagcg ccttggcgcg atgaagaccg
      541 gcgtgatgag tgcgttttcc gaaacaccag caagtgcggt ggcgccaaac ggaaccttgg
      601 tgcttgcaga cggacagcct gccacaccac agacgctcgc gcttggtgac aaagcagctt
      661 tacatggaca gactgcgatc acttcctcac ctggcggaag ctcgcggggc aagctgctgg
      721 tcatgttggg actcgctgcc tctgttctct tggtcggcgc ggtcgtgctg ctgcgcagtc
      781 gcaagccacc gccgccggct cctttggccg agcatccaca gggaaaaccg gatcgcaaaa
      841 ttccgccagc tgatccgagt ccggaaccgc ggcgcaaacc gagcgtgcca gttccgattg
      901 ggatggtgtt tgtgccaggg ggccgcttcg agatgggcag caccgatggc gaagtcgaca
      961 tggcatttaa gctctgccaa aagcgcggca gtggctgtcg ccgcgatgtc ttcgagcgcg
     1021 aaacacctcg tcatgccgtc gtcgtgcgcg atctgtttgt cgatcagtac gaagtacaaa
     1081 acgaacagtt tgtcagcttt ttgggaaccc aaaagggact caagctcgac aaaaaaaagt
     1141 ttgtaaaggg tgccaaagga atcctgatcg aagtcggcgc tgagagcgga attgtggcgg
     1201 cttcaaaaaa gctgacattt acgctgcgcg aaggattcgc aaaaaaaccc gttgccatgg
     1261 tgtcatggtc tgcggcaaag ctgtactgtg aagcgcaagg gaagcgcctt ctgacggaag
     1321 cggaatggga acttgcggcg cgcggacttg gccgccgaac ctatccatgg ggggacaacc
     1381 agcccaagtg cggagaggtt gcatttggtc gcgatccggc actgcccaac tcggcgcgct
     1441 gtcgcaatct ggaaagcacg ccggacccgg tcgggaagtc gacccaggac cgctctccag
     1501 atggcgtcta tgatctgggc ggtaacgtca ccgagtgggt tgcagatgcg tttttggaac
     1561 actatgagcc ctgccaaggt gcttgtaagg aaccgcagat cagcgacgag tcgatcgcaa
     1621 gtgctggcaa aaagaaaaac aagaagggcc cggctccgct gcatgtcgcg cgcggtggga
     1681 acttcaatca atcgttggat ggttgtcgca gcgcgggacg gaatcgtttt ccggaagaca
     1741 ccttgcttcc cacccttggt ttccgctgtg ccaaagcagc cgcaccgtcc ggtagctagt
     1801 ctgtgctcga ttcactggcg caagcggctg tccgtcggat atactagagc gatcggtctg
     1861 catgtctgcg cgcagactgt cccacatacc cagtttctgt tctgtgccca aaaggagttc
     1921 ccaatgccgc gttcgatgac gactgccacg ctgtttttcg ctgctctgtt tgtcggtgtc
     1981 tgttttggct ctgtgtcttc cgccaaaacg gcgcacacgg aaagtcagcc ctcgggacct
     2041 tccgcagacc tgcgaagcga agcgccgccg ccccacgatc cgaccaaaaa gaatccaggc
     2101 gagccgtgca aagatgcaaa ggaatgccag ccgcatcacc agtgcgtcaa agtcggcgat
     2161 aagaatgtct gccaagcgcc gccgcgtccg cagcttccgc cgggcgccgt gacctagctc
     2221 tctgtttgcc gctgccaatc ccttctgcgg agtccttttt tgcacgggta aaggcctttc
     2281 cgatgcgcag ctcttccgca aaaacagcgt ctgttgtgca gtctgcgtgg ctgcaagatg
     2341 cccgctttga tctggtcttt gtcgtgggaa ttgcgctgtt ggcggcagtc atgtccgcag
     2401 tcacggtggt ctctccggaa ctgtttttgg cgatgctgac tgtgcatacc tggctgttcg
     2461 gctacgaaca cctggccgca acgtacaccc gactgtttgg tcgtccagaa gatcgcgagc
     2521 gttttttccg tctgactttt tgggttccgc cgctcgttct gttggcactt tgtgcggttt
     2581 ctcggacctc ggggctcatc gggatttatg tggtgtattt tttcggccag ctccaccaca
     2641 ccgtcaggca aagttttggg attgcgcagc agtatcgtca gcgggccggc ggactccctt
     2701 gggacccgcg ctggcagtcg cagctgacgc tgtggtcggt tccgatttgg ggactcctcc
     2761 accgctgtgt ccagagaccg gtcgagtttc tgttccagcc gttctggttg cccccagttc
     2821 ctcgcgcatt ggaaacgtgt gcggccattg cgagcgcggg actttggctg tactggttct
     2881 acacgcgcgt ggccgcgtac cgtcgggggg aactcgcgct tggccatacg ctgtacatgc
     2941 tgtcccacct gtttgtttat ttggggggat acattctcat cgacgatttg tgctcgggct
     3001 ggctgctcgt caacgtctgg cacaacgtcc agtacattgt ctttgtctgg cattacaacc
     3061 ggcggcgttt tgcatccgga atcgaccccc aaagccgcgt gctgtcgtgg ctgtcgcagc
     3121 cgggctgggt ccggactgcg ctgtatggac tttcatcggt gctgtttgcg ctgccgcttt
     3181 actatctatt gccgcggctt ggtttgaccc ttgacaagct ggttgcccag accgcggtgc
     3241 cgtcggcgat cttgatagga ctgtcgctta cgttccatca ctacatcgtc gatgcgtttg
     3301 tctggaagcg gcgcaacgac cccaccgcga tttgatcagg agtttcggca gtctgggcac
     3361 gcgcgcttgg aagtttgggc ggatgctggc tcgcagcgtc cgcaggatga gacctatgaa
     3421 gaagcggatg gccgcttggg ggacgcagat gatgggtacg cgcaacggat gggctgcatg
     3481 gagtcttgtg ctcgggcttg tatgggggtg cagtacaaac ggcaccgatg ggactcccag
     3541 tggtgatgtc gtggatgctg cagttcctgg cattcctccc ctcatcaatg agtttgtgac
     3601 ccggaaccag accggcctga ccgatgaaga tggttcgacg tccgactgga tcgagctggt
     3661 cagccaagac acggttccgt ttgaccttgg cggctatcac ctgaccgata gcaaagccag
     3721 tccaaagcgc tggacttttc cggaaggaac ggtgattcag ccgggggcct ttttggtggt
     3781 cttcgcgagc ggaaagaatc gcatcgtggc aggcaagccg ctccatacga acttcagtct
     3841 gtcgagcggt agtccgcaag gaagctgctc gggacaaagt ggtggatacc tggccctgac
     3901 cgatcgcgac ggcgtccctg tcggtccggt gtttgacccc tatcccgcgc aatcagcgga
     3961 tgttgcatac ggactgcgca gtcctgtccc caccgcggat cgttcctatt tccaaaagcc
     4021 cactccggcg gctcccaaca acctgggctc tgccccggcg gatcgcgtgg tcatagagcc
     4081 aagtagccgc acgtttgctg ttattacgcc gctgcaagtc actctttcaa ccgcatcccc
     4141 aactgcggtc attcggtaca ccaccaatcg cgcaagaccg atagccgtcc ctggttccgt
     4201 tggtacgttt gcagccgatg cgatgaccga tacctgtacg cagactgccc acggactcca
     4261 aaatggcgat ccggttcgcg tgtccggtcc ggccccgctg acttccggaa acacctattt
     4321 tgctgtcgtg ctgtcccccg atacattcaa gctggccgtt gagccgggcg gacccccgat
     4381 tgacctgacc gcgagcggaa cttttgaagt ccggcgcgac gctgcaaatg gtaccgcagc
     4441 cacaagcgac atcatcacca ccacctatcc gcatacgttc tttgccggtg attccgtcac
     4501 ggtcagcaca agtggaacgc tgccggctcc gcttaccgct ggcactacgt attacgtcga
     4561 tgtcctgtcc gcgaccacgc tgcggctgtc gacatcaccg acgcttgtgc ccgctgtcga
     4621 tatcaccacg acgggaagcg gtagcttgac ggtttcacga actccgtccc ctacctattc
     4681 caagccgctt tccgtttctg tgaccacccg tgttcgtgcc cgcgcatttg aaacgggccg
     4741 tccagacggg ccgcttgtcg gggccatgta ctttgcgctt gatgcggcag cacagacggc
     4801 aaattccaac ttgccgcttg tacttacgca ctcttttaac accgcgattt tgaacaacaa
     4861 cgtaccgatc gacagctaca tcatgttttt cgagcccaag gctcccgaca acctggccaa
     4921 gatgaccaat cttcccgatt tggtcgcgcc gtgtacgctg gaacggcacg gatcttcgac
     4981 gggtggcgaa gccaagtttt cgatggtggt cgaacttcag gatgaaaacg gaatcgatca
     5041 gaactgctct ccgtttggta tgccggcaga gtcggactgg atgctcaacg cgccgtatcg
     5101 ttacgaccga tcgatgatgc acaacgacct tctgtatcgg ctgagcaaca acgccggtcg
     5161 ctgggccgtc cacaccaagc tggtcgaaca cttccacaac gaacagtccg cgcccgacac
     5221 catcgaggga tcgtttaccg gcgtcgatta ctttggcgtc tacagtttta tggaaaagat
     5281 aaagcgcggg ccaagtcgcg taaacgtcga agggctctcg atcggtgaca gcaccacccc
     5341 gaccattgca ggtggctaca tcttcaaagc cgaccggctc gatccgggtg aagtgggaat
     5401 cgtgccgctt tcgggacaga gcttcggcgg catgggcggc aacgtcatgc cctgggttta
     5461 tccgcgagag ctttcccccg atccgttcaa agttgtgaac ctggcgcaga gcaactggct
     5521 gcgcgggcac ctgggcgaag cctggtcggt gcttggcagt gcaaactacg ccgatccagt
     5581 aaacggctat gccaagtatt gggatgtcgc cgcgctcatc gacaaccaca tcctcaacgc
     5641 cgccagcaag aacgccgacg ccttccgctt gagcgcgttt tggcacaagc cacgcctggg
     5701 caagatcacc gccgggccgg tctgggacta cgaccgcgcc atgggatcga ccgatggccg
     5761 cgactttgac tatggaacgt ggaccggcgt cgccggcttt ttcaactatg cgtggtatgt
     5821 ccagatgttc gccgatccga acttccgcca ggcctggatc gaccggctcc acgagctgcg
     5881 ccaagggccg atgtccaccc aagcgatcca cgcgcacatc gacgagtttg ctggcctgct
     5941 caatccgggt gatggggccg gcaccccggc caagcgctcg gcggttcgct ggccggtgag
     6001 cgcgccgcgg acgaccgcaa acaacaccgc gatcaccaac aacctgtttg atggcacata
     6061 tcccggcgaa gtggcctggc tcaagtactg gtgggaaaag cgcctggcgt ttatggacgg
     6121 cgagctgacc cgtcccgcgg ttgcgaacat tccgtctggc aaagttgcca tagggaccac
     6181 cattacactc agctcgccga gccagtcgct tcccggcgtc aagctgtact acaccaccga
     6241 tggttcggac ccgcggcccc ccgcgcccgg tcccgtgctt tccgccaaag ccatcgagta
     6301 caccggaccc atcaccatca gcggcccgac gcaacttgtg gtgcggacct acaatcccac
     6361 gccggccacc gttgctgtca ccaccaactg gagcgcgaag acggtgctcg actataccca
     6421 gtagacgctt ttttcaggac tgcccgcgct ccgccgctag cttcgaccgc agcacttttt
     6481 ccacttgagt ccacttccgc aagtacacgg gtcattgcgt gaaggggttt gggccgcgtg
     6541 tttcaccgtg cctgcgtttg tgcggcgtag ctcgcttgtg ccgtcgacgt agacccagct
     6601 tccgttttcg cgcacaaact ggctgtcttc gtgcagcgct tgcgaaacac cttggctgcg
     6661 aaagcgcgcc acaaacgcca ccctgcccgt gtcgtcggtt tccctgcccg cttcggtccg
     6721 ttcaatgtga agacccagcc attcggtctg gtcgcccgca aggtcgagct tgcgcggacg
     6781 ctgcgccttg gcccacgtca gcagcagata gtcactgttt cgcacgacat aggcgctgta
     6841 gcgcgatcgc atcagcgctt ctgccgtcgg ggccgttccg gggtttgcgt ggtagatgcc
     6901 gcagcagccg gccagcacgt tgccgcttcc acagggacag ggctgcatca gcgcgctccg
     6961 gtttcgctct gtcgcttttg cgctgcctca gtgataagca gggccagcag cgctggaccc
     7021 gactggaaca aaagcaccgt ctggcttccc agcgaatagc agccgtagat tcccgccacg
     7081 atgacgcagg ccaggctgaa gcgacgcaga agcgccgatt tggtcagcag cgccgtcaaa
     7141 agacccgcag ccagaaagcc gttataaaga ccctggtttt gcgccagcgt cgcgcacgca
     7201 tcggccttct gctgcgacat ctgaaatgtt tcgagtccga ccggggtgcg gaacagaaac
     7261 atctccagga tcaaaaacca caggtgttcg accgcgacca gcacaaccaa gagggttccg
     7321 acaagctgca tggtgcgagt atgccgcaaa ccaagtgacg agcgcagtgg gccggcccaa
     7381 ccgctgcgat cttcgtgccg caatcgtggc gcctacgttg ccagttcgta atgtcgcaag
     7441 ccttcggtgt gtgcgcaggg gagagcgcgc ccctgcggta tgcaaccgtc gcagatggtg
     7501 cgtgtatctg cgcagcgcga aatcgcgtta gacgatggtt cccgcgtctc cggcaaacca
     7561 gaggtttgtg ggggttgtcg atacggcgcg cagggtgcgg gtcgtgattc cgtttgtgtt
     7621 tggatcgagc agccaatcgg cgccactcgt gctcattacc gtgccttgat cgccgacgac
     7681 gaagatcggg ttgccggtag agctggccgc tgggatcgat cccagcgcat acaagttctg
     7741 ggtcttgcgc gcgctggatg cgacggtcca aaacacgcca ttaaagcgga gcagggtgcc
     7801 gtagttgccg acagcccaga tgctggtgtt ttctgcgccc cacagcgagt tgagcgcttc
     7861 tttggtgccg ctgtcgactg gcgaccagct cttgccatcg tgacgcagca gcgtgccgct
     7921 gtcaccgacc gcccacttgg cttggccggg aatcgcgaaa atgtcattga gcttggcggt
     7981 ggttcccgtt gcccaagtgg tcgtcttgcc cccgcttacg ctaaggcttg tgccgccgtc
     8041 gccaacgatc cagacctcgc cttcgggatt tgcatagacg ccgcgcaggg acagcgtggt
     8101 cggaatcgaa gcgccaagcg atactttgtt ccagcttgtg ccatcgtaat gaagcacggt
     8161 gctgccatcc ccgacggccc agatgttgga tgctgaggtt ccgctgatgg cccacagctc
     8221 gacgctgccg gcaattggac ttgtcgtcca tgccgttccg ttccagtgcg cgagcgtgcc
     8281 ttgaccaccg accacccaga aatccgtggc gctttggccc catgccgcgc gcagatcctg
     8341 gctgcccatg ctctcaacac agctccaggt gtcgctgatc agccgactga ccgtgccact
     8401 gttgccgaca aacaccgctg agcccagatc ggcggacacg attcccgaaa acatgttcgg
     8461 aagacgggcc cgcctgggtg aagaccagta cgtcccctcc cagtgcagga tggcgccgtc
     8521 accggatgcc cagatgtcgt cgttgtttga tcccttgata cggcgcaggt gcagtccctg
     8581 cggacgcggg tgttcccagc agacttggtc attgctgcat acggtgtttc gtccggcccg
     8641 tactgtgacc tgcactttgt cggccccgat cgtgacaccg cagcctgctg cgctttgggt
     8701 gcagcctgcg acccctgcga tataggaatc cggcgcaatg tcccacgccg tgtcgctctt
     8761 tagctccagc ttggttccaa acgggaacgc tttctgcatc gatgtgccca cgatcaaatc
     8821 gatcgggcac atcgcttcgg ggggctcggg gggcgaaaat gtccacgcgg ttccgtccga
     8881 aagcgtcacc cgcgcggcgc cgtctccttc cttgcggacc acaagcgtgc agccctgttc
     8941 atcggccaga tcgactgacg cgctgtagtt gccaggtccg gtgatgtcga cggatgtttt
     9001 ccccgcgcgc gccgtgcagc catcactccc ttgcgctgaa atcgcgattc ccagcgttcc
     9061 caccgctgtt tcggccagcc gcagctcgat gtgatcgaga cgacgggtca ctgtgtcgag
     9121 cgagtttacg gacttttgac catttagcgt tgtctgtacc gtttcagtca caagatccga
     9181 cgtgatgccg tttaccgtga ccatgacaat cggctgactg ggcggcgtgc agccatgcag
     9241 cgcgatgccg cacacaagcg cagtcagtcg ggacatccct gcctggatgg gaagacggaa
     9301 gtggcgtagc cagggaatca tcggcagcgc tccttttcgg tcgcaaagtc agggatcttg
     9361 gcgcagcaca agccgcagcg aggcaacgcc cagcggtgtc gcggtcgcca cttgcatcga
     9421 cggaaacagg gcacgggcac gctctttggg acaggttttg acagcgtccg gaatcggcgt
     9481 ttcacaggga agctgaagct gatcacagag cttgcgatcc gcctgttcca gtggctccgt
     9541 ggaagcgatg acccgcagat cgtgatttcc cgctggattg cgcagataaa acgaaccctg
     9601 cgccggaatg ctcaccggac agcgtgctgc gatgcgcgcg ggttcccctt tgcgtggaaa
     9661 cagaatgctg ggcgtgccgc ttgggccata cagaacgacg tacagatagg ctggctgtga
     9721 cgcccggacc aggaagtaca ggtgatcacc agcatgcagc gattccgacg ccaaaagagg
     9781 ccgctcggta cttccctcgc tcagcatcgc ggtaagcgag acggtgagcg gaccgcttgc
     9841 gggcagatca tcggtggcgg ctcccagtcc acgggtctgc tggctgccgt ttggccgggt
     9901 gtgtttgcag ccaggaagca gacagaaggc gagcccggct gccaggtaca gcgcctgtgc
     9961 gtgctgcgtg actttgcgaa ctgctaacaa agacagactc ctgtggctgt ggattcctgg
    10021 ttggaacaca gcgttagaag cgtggctcca aaacattttg gctgccggaa atcgtcagag
    10081 acagtccgac cgcaagcccg attcccaccg ccagtcccaa agacaccccg cccgcacccc
    10141 acagcagtct tcgccgcacc gcttgtttgg gatttggcag caccccttgt tctggcagtg
    10201 ccgcaacggt gtaatcggga accgcattgg gcttggccag tgtcggtgtc tgtgccccgg
    10261 cggtcagctg tgatacccga acgcttgtcc aagtttccag ctcgctcact gtgatcttgc
    10321 tgtcttctgc atagtctgcc acgccgcgca gtccttcgac gagtgccttg gtaaagacgc
    10381 cgttgttcca atcagatgac tctcttgcca cctgatcgcc ggtcgatgcg gcaaacacca
    10441 cgatgcgatt ttcgctggtg agatcgttaa taaaccgatt caagctgcgc ttcccgagca
    10501 cgttgccaga gtgacaggtg tcgagcatca agaccacgcg ccctggaatc tgccgcagcg
    10561 catcttgcag ctcgctgcct gacagcatcg tcgcgcgatc cgcctgggcg tccgttggca
    10621 aaaagtaata catcccgtcc ggatcgtttg ttccatgtcc ggccaaaaac accaccgcgg
    10681 tgtcgtcttt gccgacttgg gtggtcagcc agtgaagtcc ttccatcacc tgcgaacgaa
    10741 tcgcctgccc atcggtcagc acgcgcgtac tgatttcgcg atacaacatg tcacgctgtt
    10801 tttgcagggt ggtggccagg tcttttgcat ccttggcggc aaagcgcaaa gacggaatgt
    10861 cggtggattg atagcggccc accccgatgg ccagcacata cagcgcgggc cggcgttcgg
    10921 gaactccttt gcgcaatcgc agggtcagcg gctgactcga cgtgctaccg ctgcgcgcga
    10981 cgatcgaaag caccccatct ttgtggggcg ggcggatctg aagcgtgtaa cgactggcgg
    11041 tggccagatc ccgtccttgc gccacggtca gcgctttcac cggctcgccg tcttgcaaaa
    11101 tcgccagctc gacttcgccc cccgccggat cttcgacctg cgcttcgacc gtgatcgatc
    11161 cgtccttgtt caccgtcgaa gaaagcagct cgatgtgcgg tcgtggcgtc gtgctgcgta
    11221 gctctccggc tgccgcggcg gggttttccg tgctgcgtgc ggtgggactt tgggcccaag
    11281 ctggctgggc cgctaaaagc tgggctgcgg tgccagcgag cagaaagcga cagcgtctga
    11341 cactcttctg catcagttaa gacgctaccg aaaacgcgcc agatggcctg ttgatcgtta
    11401 cctcgccctg ctttcgcttt tttgaactac ctgactggcc tgccgcggac aaaacataca
    11461 cggacggatt ggagagcgcg ccgctttcca aaactttcac cagcaatcct cctccttccg
    11521 gtggggggac caagtctgcg gcgcggaggg gaccctgcgc tcgcgcaaag aaggagtacg
    11581 tatcgatgcc cagctcgctc acttacccag gcgtatacat agaggagatt cccagtggca
    11641 tccgcaccgt cactggggtt gccacgtcgg tcactgcatt tgttggccgt gcccagcgtg
    11701 gacccgtcgg ggaaccggtg gtgctcagca gcttccgcga ctacgagcgc aacttcggtg
    11761 ggctgtggct cgacagcacg atgagttttg cggtctatga cttcttcaaa aacggcggca
    11821 gccaagcggt gattgtccgc gcgttcaaga agccaaccac caccgcgacg gacggatcga
    11881 ccaccagcgc gagcggagtc gctaccttca cggcggacac cctggggctt tcggccaaaa
    11941 gccctggcac ttgggcggcg aacctgctgg tctccgtcga ctacaccgac agaaccagcg
    12001 atgtcagcgc cgaaattgcc aagaacctgg gaattgccac tagcgatctg ttcaatgttg
    12061 cgattaccta ctttgcagcg tcgggggctg tgaccgagcg cttcgtcaac gtgaacctaa
    12121 aggacaacgt ccgtcgcgtc gaccgggtgc tggaacagaa gtcgctgttt gtccgcgtca
    12181 ctgccgggag cgatgggaat ccgacgctgc caacggcagt ccctgcggcg agcaccgcca
    12241 acgcgaccac cggggaagtc ggcgttcgtg caaacacggg cactgcactc gacagcttgc
    12301 cactcacgtc gactgagtat gacttttcgg ttctcgacaa gaccgatacc gtcaacctga
    12361 tctgcgttcc gcccgacacc cgcggcggcg ataccgcaaa ctcggtgtat tcaaacgcgc
    12421 ttacgtatgc ggtaaagcgt cgggcgattt tgattgtcga cccgccggca gcgtggagca
    12481 gcaaggccgc acaagacatc aagacttctg accttggcct gacgggtcca gccgcgcgaa
    12541 acgccgtcgt ctactacccg cgcattcgca aaccaaatcc gctgcgcagt gatctggtgg
    12601 aagagtttgc gccgagcggt ctgtgcgctg gaatcatctc gcgcacggac acccagcgtg
    12661 gcatttggaa gtcgcccgcc ggcattgacg ccgccctgta tggcgtcgac agcgtgcttc
    12721 ccaagctcag cgacagcgac agcggaacac tcaatccgat gggcgtaaac tgcatccgca
    12781 gctttccggc cagcggtcag gtcatctggg gcgcgcgtac cctgcgtggt gccgatgcgc
    12841 tgggtgatga gtacaagtac ctgtcggttc gccgcctggc tctgtaccta gaggaaagca
    12901 tctatcgcgg cacccagtgg gcggtgttcg agccgaacga cgagccgctg tgggcgcaga
    12961 tccggctcaa catcggaacc tttatgcaga acctgttccg ccagggcgct ttccaaggta
    13021 gctcgccgcg ggatgcgtac ttcgtcaagt gtgacaagga gacgaccacg gcggctgaca
    13081 tccaggccgg catcgtcaac atcgtcatcg gttttgctcc gctcaagccg gcggagttcg
    13141 ttgtcatcaa gttccaacag gccgctggcc agagctcgta ggagtgaccg cctatgcccc
    13201 agtttaccgt caacacgcac cgctttgagc cgtaccaaaa ctaccgtttc cgcatcaagt
    13261 gggacggtcg ctacgtcgcc ggagtcacca aagtgtcttc cctgcggcgg tccaccgaag
    13321 tcgtcaaatt ccgcgaaggt ggcgacccct cgtccagccg caaacttccc ggtcgcaccg
    13381 agtatgaagc gatcacgctg gagcgtggcg tgacccacga tgcagagttc gagcgctggg
    13441 ccaacaaggt ttcgacctac ggcgcgggcc ttggggccga gcgctcgctc aaagacttcc
    13501 gcaaggatgt catcatcgag ctgctcaacg aagccggtca gattgcgatt tcctaccggg
    13561 tctatcgctg ctgggtttcg gaataccaag ccatgcccga tctcgacgcg agcggcaatg
    13621 ctgtggccat cagccacatc aagctggagc acgaaggctg ggaacgtgac accgcgcaga
    13681 cggaacccgt cgagccgacg tttcagcttc ccagtaccta gtgatggagc agagcggttt
    13741 tggccatgat ggcaagtgaa gcggagatcc tgcgcggttt tgatctgtgt gacgggcaca
    13801 gtgatgtgga ccgcgccgtg ctgatgctgc tgtgggggta tccagagcgg cgcctgccag
    13861 agcttttggc tctgcccatt ggcgcgcgtg attcccttct gcttgcgctg tgtgaacgga
    13921 cgtttggcga tcgtgtgtct ggatcagcac tttgtccggt ctgtcagcag cgcagcacgt
    13981 tttttcttcg cgcgtctgat cttcgtttgc caccgcaaca gcaggaactt cctttggtct
    14041 tgcggcacgg cgattgggaa ctacactacc gattgcctac aagctacgcg ctgcgcggtg
    14101 cggcggcgtg tccggatgtt gaatctgccg cagattccat ccttcgctac tgtcttctgt
    14161 ctgcccgtca caaagagacc cccgtatcct gcaccgacct accgatcgac atctgccagc
    14221 gaatcggtca ggcattggct ttggctgatc cacagagcga agtccttctg tctttgcgct
    14281 gcgcgcactg tggtcacaac tggcagtccc ttttcgacat cgcccgcttt ctgtggacag
    14341 agctgtctca caaagcccag cgagtctttg acgaagtaca caccctggcc agcaagtacg
    14401 gctggagcga gcaagagatc ctgcacatgt cgcctgtgcg ccgtcgccaa tatctacaga
    14461 ggtgcggaca tgagtaactt cttccagcac cttgcacttc gcgcgctcgg gcaggtccgc
    14521 aaaagcgatg cccagcccgt cgttcgttct cgctttgagc cagacagcca gagaagtcgc
    14581 aatctgcttg atgaagtcgg ccagccggat ccacaggaac atgaaaatgt tccggatctg
    14641 ggccacacgg accagacatc cctggccaat attcgaccgc gtgtgactgc gggccaggcc
    14701 ggtttcacgg tttctgcttc tgaccgaact gcggaaccat cgctgcgtgc gtctgcgcag
    14761 gtggtcccgc acggggacac accaaattcg cacgatgttg cacctggccc agaagacggc
    14821 ccgcagtcgt tggaactttc ggaaccgtcc ttggctgggg ccaccccatc gcgacacgat
    14881 gtgatgccgc gtggttccaa ggaaccaggc ccagatccat tttccgtgga ccccttggcc
    14941 gaagttcccg accccggtgt gtccgttcct tcatcgaaag acaagccgcg caccaccact
    15001 ggcaagtccg cggatttgca ggacgcagac ttcggcgatg cgcaggatgg ccgcagcaca
    15061 aatcttccgg catctgaccc aaccagcgat tcgcagccgt ctgggacagt gggcaaagcg
    15121 tccccagatt cgattcggca gcgaagtaac aatgccgacg ctgcggggac cacatcggaa
    15181 gggcaaccgc gtcaattatc ccgcgtgtcc ccactttcgg cagaacctga ccgcagttcc
    15241 gcaaacagcg agcgtgtacc gagcgtggag gaaacggaat cagattccgg cagcggcgct
    15301 tccggggatc gtgccgcgcg cacggtggct gacccccgtg tgtctgttgc ggggccgcgc
    15361 ttgccggcaa gccatgcatc acgcgctggg acgcggaaca cgggccacag tgaaacggaa
    15421 gaagtgcagg ggacagactt tgcttctgct ccttcttctg ctttggcgca gaccgatgta
    15481 tcccatggcg cgggtcaggg gatttcgctg cagacggcca aaccgaagtc cgaatcggct
    15541 gggccatccc agtcggtgtc tgcgccgcaa gtccacattc gcatcggacg aatcgaagtg
    15601 cgtgccccgg cgccaaagcc tgccatggtg caagcgccgg ttccgccccc gcggacgcct
    15661 tctgtttcgc tttctgacta cctcaagcaa acccgaggcc gtggatgagt agtacgttgt
    15721 ccgtagcggc tgtcactgcc gcccttcgca accgtttgac cgtcgtgtcg agccccttgc
    15781 cgggagagac caccacggat ctttcggata cccaggtcac gaccctgccg ccagacaaag
    15841 cggggcttca ggaagaccac aaccagatca atctgtttct gtaccagatc cagcccagcc
    15901 cgtcgggacg caacctggat tcccgtggtc aggcgggtcg caagccggcg ctcgccctgg
    15961 aacttctgta ccttgtgacg ttttacgggc gcaacgcaaa cgagatcctg acccagcgtc
    16021 tgcttgggcg ggccatgagt cttttgcact ctgcacccgc gctggcccca gcagagcttg
    16081 cggcggcgct tccgggcagt gacctcgaca aagcgaccga cctggtgcgg ttggttccgc
    16141 acctgatgcc caacgaagaa atggtccgtc tgtggggaac attccaggtg aagtaccgcc
    16201 tgtcggttgc ctatcgcgcg tccatcattt tgattgacca cgaatacccc gacaccatct
    16261 tgcctgcgcc gaccgagcgc cgcattacct tgggcccgat tccggatgga ggcagcttgt
    16321 gagcgatggc ggtccttcat cgcctgttcc cagcgtccat gatcgctggt tgagcggcaa
    16381 tcagcagtat ctgctgcttt cgcttgcgcg ggttcgcaag gcgcttttgg attatctttt
    16441 gcgcagccag ccggacgcag tcgtaaacgc ggtgccggag cctttgccac aggcggtcga
    16501 tgcggattcg cttgcggctt cgatgccggc acccccggcc ctgctttcgc tgtgtgcggc
    16561 gtttgagctg tccgcgtttg aacgggatct gctcttgctt tgtgtcgggg tcgagctgga
    16621 gcccgcgttt cgtcgtttgt gtcagaaggc tggcggcacg gcgcagccga cgttccagct
    16681 tgcgctttcc gccttggcgc agccgcacct aagcgcggtg tctccctcgg gtccgctgcg
    16741 ccggtttggg cttgttgaag cgttgtcgag cgaaacgctg accgcaagtc cgctgcgcat
    16801 cgacccgcga attttgagcc acctgaccgg gctttcgctt gtcgatgaac gtctgcttgc
    16861 ggtgggccgg ctgctttcgc cacctgatga accgcttggc accgcgcttt cgtcgcttgc
    16921 gcagcagatt gtgcggctgt ggtccgtgga cacagaggac gcgccgcccg cggtttcgct
    16981 gtttggggca gacggcggcg aacggctcgc gctggcccag cacgcctgtc agccgctcgg
    17041 ccttttgctg tttgaagttc gctgcgccga cttgccacac agtcctgcgg aacgtgccca
    17101 gcttgccaag ctgtgggcgc gcgagcagtc acttttgcac attgcgcttc tgctgagtgt
    17161 ggcggacgga gattcgcccg aaacggtgcg ccaagcgatg ttgtggatcg ctcagctacc
    17221 gggcgctgtg ttgtgcagca gtccgacgcc cctgcgtgat gcgcaaaagc ggatggtcgg
    17281 gatcgaagtt ccgcgcatgc tgccggatga acggacagag ctgctcaccg agctgttcga
    17341 ggaactggac ccgcagcttg cgtcgatgcg cggtccaggg ggggaatcgc ttctgtccga
    17401 gctgtcgatg cagttccaga tcgggcgtga agccgtggtg gctgcgtgtc agcgcgcgag
    17461 cgggatgtct ttgccgctgc cgcaggcgct gtggcagtcg ttccggatgc aggccagacc
    17521 ccatctggat gagctggcgc gccgggtcga gcccaaagtg acctgggatg atctggtgct
    17581 ggccgatgag gtcaaacaga agctgcgcga gctgcttgcc catcagcgct atcaagtcac
    17641 ggtgttaagg agctggggga tggcgggtac ggcgcagcgg ggacttggcg tgtcggcgct
    17701 gctctgcggc caaagtggta cgggcaagac gtttgctgcc gaggcaatcg cgcacgagct
    17761 acagcttgac ctgtatcaca tcgacttgtc gcagatcgtc agcaagtaca tcggcgaaac
    17821 ggaaaagaac ctgcgccggg tgttcgatgc ggctgaagag ggtggggcgg tgctgctgtt
    17881 tgatgaagcc gacgcgctgt tcggcaagcg gtccgaagtc aaagacagcc acgaccgcta
    17941 cgccaacatc gaagtgagct acctgcttca gcggatggaa tcgtaccagg gcatcaccct
    18001 gcttacgtcg aacctgcgct cggcgctcga cagtgcattt ctgcgtcggc tgcgctttat
    18061 cattccgttt ccgtttcccg atgcaaacca gcgcgcagag ctgtggcggc gggcgtttgc
    18121 caagcagacc ccgaccaggg gacttgtgtt tgaaaagctg gcccagcttt ctgtgacggg
    18181 cggacaaatc cgcaacatcg cgctgtgtgc ggcgttcttg gcggcatcgc agggtcagcc
    18241 ggttggaatg cggcacattc tcgacgcggc gcgcagtgaa tatcagaaaa gcgagcgacc
    18301 cctaaacgaa aacgaagtgc gggggtggct gtgacgcacc aggtcttttt gcatatcgat
    18361 gagattgttc tcgatggggt cgaggggacc ggcgactttc aagccgagct agagcgcgcg
    18421 ctcttggcgc aactggcagc gccgggtctg gcggctctga tggtcggtgg cggcgacttt
    18481 cgtgagcttt cgctcggcca gcttcagctt ggtgagctgc ggatcgggat gtcgacgctt
    18541 gggtcggcgc ttgggtcggc gcttgggtcg gcactggttt cgcggcttcc cgcgtcgcca
    18601 ccgcaagtcg ggtctacggg ggttgcgctg ccggggctta cggcgggtcc aaatcgtggc
    18661 gttgcaggca aggagtaacc atgggcattg cagacaaagt gagtcagacc gctctctctt
    18721 atgccgagca ggcgatttcc agcattccgc cgctgcgcaa aggggcgctg attcatgggc
    18781 caatgctgat tccgtttcag tacaacccgg aaaagctgac gcgcacgctg acggcgagca
    18841 gcatggggac aagtggcccg gatctgttgc ggctgaaggg accgcccggt gaaaaaatcg
    18901 cgctcgagat tttgctcgat gcggcggatc aggataaccg gcagagcggg ctgcagcttg
    18961 cggacggcat ctatccgtct ttggccgcgc tggagatgat catttatccc agctcggcgc
    19021 gggtgctggc caacgcggca ctgtctcaga tgggaatcat cgagctgatt ccgcctttgc
    19081 cagtgatgac ggtgctggtc tggggttatc ggcgcgtggt gcccgtgcgg atcgagtcgt
    19141 tcacggtgac ggaagagctg tttgattccg cgctcaatcc gctgcgcgcg catgtcggcc
    19201 tggggcttca ggtgctgact tacgaagacc tggggcttct gtcggcgggc ggcatgctgt
    19261 cgatggcggg acatgtgcag cgggaagtgc tggcggcgtc cgcggcgatc cgttcgacga
    19321 ttggtgcggc gagcgtgacc gggctggaca tgaacgtcgt cagcaaggcc ataaaaggga
    19381 ttctgtaatc gctaggtggg gggacaaacg atgctggacc caaacagccg ctactacaac
    19441 atcgagacgg cgcagcttga acaaggcggg cgcagcgtgc gctatcttac gcggcggatg
    19501 ttgccggccc tggccgcgca gacaacgcta gaggaatacc aagcggcggg cagcgagcgg
    19561 ctcgatctgc tggccagcat gggctatggg gacccgctcc aggcgtatcg gctgatcgac
    19621 gcaaacccgg tgctgcaccc aagtgagctg ttggtggccc ggcggcgcat tcggatcgcg
    19681 ctgtcgcgac tttaaccaaa gtggtgggaa cgccgaaacg agcgagctgg caaaggggga
    19741 tagcgtgcag gccaatcagt tgggcgtgcg ccttgtgctg tgggcgggaa tcgttcctgc
    19801 cccggtgccg cagtcggtgc tagaagcgat cgagaagctg gaaatcgagc gcagcggaga
    19861 cgacgtgggc ggttttcagc tcacgctgcg ggcggaaaaa accgtggggg tggagttcga
    19921 tcttctgcgc agtccgcagc ttttggtggg gagccgggtc gtcatcggcg tgctgatggg
    19981 cgtgacgccg gaatggctgc tggatggtgt gatcacccat cgcgagcttc tgccgagcga
    20041 caaaccgggc agcagccggc tctgtttaac cgggcgtgat ctgtctgcgg tgatggaccg
    20101 gatggatgtg gtgatgagct atccgggctg tagcgattcg gtgattgcag cggcgatctt
    20161 ggttcgttac gccccctatg gcgtggttcc ccaggtcagc ccgacggtgg atgtgccact
    20221 gccgaccgag cgcgtgccgc ttcagcacga aaccgacctc cgctgtcttc ggcggctggc
    20281 gcgtgacaac aactacgtgt ttttcatcga gccgcttggt tttggcgcaa gccgcgccta
    20341 tttcgggccg gatcttcgct ttgcgctgcc tttgtcgcca ctgcgcgtcg acatgggggc
    20401 gcgcagcaat gtcgatgcac tgacgttcaa gcaagacgga gatcgcgcag agtttgtgga
    20461 aggacgcgcc tatccgccag ggtttcctgt cgcggtgcca atcgtcggtc tgccgctgcc
    20521 gcctgtgccg cctttggcgg cgattccgac cctgcctctg cactatcgtt ttgacggatc
    20581 actgaccgca cgcagcattc cgcgcgccct gcttgcagca acgggggccc aggcccgcgc
    20641 caaccatcgg gcggtggagt gcgaaggaac cgtggacacg gtgcgctacg gaagtgtcat
    20701 gcagccgcgc cggctcgtcg gtgtgcaggg ggctggatac tcgtttgatg gctattacag
    20761 cgtcagcaaa gtccgtcacg aactgagccg gcacaagtac acccagaagt tttccctcgt
    20821 ccgagagggc gttggctcgc taaccccggc ggtgatgtga tggcggacag acctacgttt
    20881 tatggcaagt atcaaggcgt cgtcgtaaac gtgttagacc ccgagcggcg cgggcgcatt
    20941 ttggtacagg tgccggcggt ctacaaagac agcgtcagcg gctgggccga gccgtgtctg
    21001 ccactggtgg gaatccggtc cggacttttg gcgctgccgc aggtcggtgc caaggtctgg
    21061 atcgagtttg agcaaggcga ccccgagcgc cccatctggt gcggcggttt ttgggcccct
    21121 ggtacggccc catcggccat tgcgccgttg caaaccgtgc ttcagaccac tggcggcaac
    21181 gtcatcttga ttgatgacac gccgggggtg ggcgggatca cgctcaagac cattgcgggg
    21241 gatacgttca agctttcgcc ggcgaccggg atcgcgctgc aaaccttcac cggggaaaaa
    21301 atcgaggtgt cgcccgtcgg catcaccatc cagagcgcgg cgggccacag catcatgatc
    21361 aatgccgccg gaatctgcct gtcgacttcg acgggggcga ctgtgcagct gaccggcaac
    21421 atcgtcgaca tcaacgcggg cgcactggag gtcatctaat gggcgcacca ctgcttcatg
    21481 ccggcaacca aatcacctgc gtgcacggcg gccaaggtga agtgatcttc accaactttc
    21541 gggtcagcac gcgcggcaac tactttatga ccgtgggcga cacggtgtcg tttgccggct
    21601 gtccgggcac gccaccacca gcccagcttg tctgtgtgat tgcccagtac gcgatgccag
    21661 cgacgcgggt gtttatctgc ggccagccgg cgctgcttca gtcttcgata gcaacggcga
    21721 caccgacggg cacgccggca gttacggcac ctgcgccgcc ggcctcattc gtagggggaa
    21781 tgtaagatgg cgatgcgctt tccgtatcag cttagcagcc gtggcacgac ggcaaccgcc
    21841 agcgcttccg aggatgcgta catccgcgat ttgatggagc aggtgctgct cacttctccc
    21901 ggcgagcgcg tcaatcgacc tgactttggc agtggcctgc ttgggatggt ttttgcgccg
    21961 gggggcgacg tgctgcaagc cgcgctgcaa gccaacgtgc aggcgacgct tcagcgctgg
    22021 ctgcaagacc tggtgttaat ccaggcggtc agcgtcgacg tggtcgattc ggcggcgtac
    22081 atcacgatcc agtacgtggt ccagcgcacc cagcagacgc aggtggcgcg gttttccacc
    22141 gaacccagca gcgcttcgtt ttcggcatct gtgtccgcgg gaggtgcgtc atgagccaag
    22201 acgcaagcca agacaccaca aggacgagca ctgcgacgac gcaagacacg ccggacattg
    22261 actacctaag ccgtgacttt gcgagtttcc ggcggcagat gctcgaccgg ctggcgacga
    22321 tttcacccgg tacagcgagc gggcacccag ccgacatggg caccgtgctg gtcgaagtgc
    22381 tgtcgcaagc ggcggacgag ctgtcgatgt ttcaagatgc ggtggcgacg gaagcctatc
    22441 tggcgacggc gcggctgcgg acatcggtgc ggcggcacgc gcggctgctc gattatcggc
    22501 tgcacgacgg ctgcaatgcc agggtttggg tgactgtcgc agtaagcgat gctgccgatg
    22561 gccaggtgtt gccgcaaggc acgcggcttt tgagccgggt agaggggcag ccgacgcagc
    22621 ttggtgaaga cgacatccgc agcctgcgca gccgtggcgc gctggtgttt gaaacgatgc
    22681 agagcctgct tttgcggagc gcgcacagcg tgatggcact ggatgtgcaa gcgggtccgg
    22741 tgttggcagg cgcgcagtcc gcacagcttg tccagtcgca gacgaccaag ctggccctgc
    22801 gcgttgggga tgtcgtcatt tttgaacagc tcgcagttga agcgggggcc gctgcggcac
    22861 tggacaggcg gcatgcggtg cggctcacca gcgtgaccga cacctcgacg tcgactgcgg
    22921 tgggggcctt gaagatcggc tggtctttgg acgacgcctt gccgtttgcg ctcgaccccc
    22981 ggcgcaccca agtgctgggc aacgtcgtgc tggcggacca tggctacacg ctgcccgacg
    23041 ccgagctgat ccagtttgaa aacagccggg cccgtcccca gcttctgcaa ggtccgctga
    23101 cgtggcagac gatggttttg tcgggcgggc aagcgctgcg gtttgatcca aatggcgcgg
    23161 cggcttctgt ggtgccgacg gagatcctgc cgagcaccat gataagtccg gtcatttcgc
    23221 ttgtggatga agtgggacag ccctggcaag cgcggcggga tctgctttcg agcgcggcgc
    23281 tggcgcgcga ctttgtcgtt gaaacagagg acgacggaca gacgtatctg cgctttgggg
    23341 atggcgtcta tggccggctt gcgcaaggcc cgctgtttgc gcgctatcgc attggcaatg
    23401 gcagcaccgg aaacatcgga gcggactcgc tgtcgcacat cggatcgctt gtgccgggca
    23461 tccgcagcgt ccgcaatccc ctgccagcgc ggggcggcaa agatcccgaa ccgctcgatc
    23521 gcgtgcgcaa cctggcgcct cggcagtttc aggtcgccga gcgcgcggtg acgacggaag
    23581 actatgcgca gattgcgctg cgcttcccga ccgtgcggga agtgcgggcg atgcgtcgct
    23641 acaccgggag cttttttacg acgcggctgt ttgtggtgcg ctttggcggt ctgccggtcg
    23701 atcaggcgtt ctgtgacagc ctgctggact ttctgtcgca ctatcggatg gcggggcatg
    23761 tgctgcgcat cgacgggccg gtcaagattc cgctcgacat tcggctgacc gtgggggtca
    23821 agtcgggaca cttccggggc gacgtggtgg cggcgctaca tgctgcgctg ggtccgggga
    23881 cgagcagcaa gccgggcttt ttccatcccg ac
//
LOCUS       DAORGN010000058        23693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_840_length_23693_cov_6.507911, whole genome shotgun sequence.
ACCESSION   DAORGN010000058 DAORGN010000000
VERSION     DAORGN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23693
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_23693_cov_6.507911"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKL17_08650"
     CDS             <1..1044
                     /locus_tag="PKL17_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNK44837.1"
                     /translation="DAYGGHAVASTGHCHPKVVQAISAQAAQLLFYSTALPHPGRERL
                     AEAIVSHCPSALRRVFLVNSGAEANENALHLARKITGRQHFVSIEGGWHGRTAATLAV
                     TDGEKYMAGARRAGIPLSVKVPWNNPDALKQAVSESIAGVILEPVQGLTGARDLPPAL
                     LQAARQACDKHGALLIFDEVQCGVGRCGAFTAAESFGVTPDVLTLAKGLASGLPISAV
                     VTTDAVAQHIKQGDLGSTFGGGPVPCAAALATLQVIDEERLIENAMAVGDFLAKGALS
                     LGQDKVVATQGRGLLLGLRLAKPAAQVQHALLAHHIVSGTSSDPQVLRLMPPLTFSKS
                     EAELVLSALSEVL"
     gene            1045..2094
                     /locus_tag="PKL17_08655"
     CDS             1045..2094
                     /locus_tag="PKL17_08655"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HNK44838.1"
                     /translation="MVASTQIPARKKRDFLDMADLAPLALAKLLDLAAQLRRGDAKHK
                     DALAQKVLAMVFLDPSLRTRTSFEVAMFLHGGHGVVLEPGRTSWAWETRKGVVMDGTT
                     VEHIAEAARVLGRYGDAVALRAFPRGGAWSEEREDRIIHDFAAYCEKPVINMESVRRH
                     PCQGLADALTIRDRLPNPKGRRCVLMWAYHPKPLPTAVPASAAIAAASQGMALQIVHP
                     PGYELDQDDMATIRSVASQNGGSVDVSHDLDGSLHDADVVYVKSWGAISHFGRPSEEA
                     ALRSPYRDWRMTKDRSEMLHPDGFVMHCLPVRRNIEIDDAVLDGPRSAVVDQAENRLH
                     AQRALLIDLVSDLAL"
     gene            2117..3061
                     /gene="argB"
                     /locus_tag="PKL17_08660"
     CDS             2117..3061
                     /gene="argB"
                     /locus_tag="PKL17_08660"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNK44839.1"
                     /translation="MSIEPAKGIAGLKGALRYVRAYRDQIFVIKLGGEVLERAESLSG
                     VAAQLSLLSSLGVHLVIVHGGGPQASQLTRRLGGEPKMIAGRRITDDTALSAVVMAVA
                     GQLNVQVLSALRAHHVRAVGLSGVDAELVTAHKRPPVEVVDDSGQTQKVDFGHVGDID
                     RVDPRVIETLLASGYVPVISCLAGDNQGNIYNINADTLAESLAVALRAQKLIFMTSAP
                     GVLRDRNDPSTLVTFADPDDLASLLSSGSIAGGMRPKVEACIRAATGGVERTHIIDGG
                     APDSLLFEVFTGAGCGTMIVGRKEKATYLGVDLAALLG"
     gene            3054..4175
                     /locus_tag="PKL17_08665"
     CDS             3054..4175
                     /locus_tag="PKL17_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNK44840.1"
                     /translation="MASTTARLDEVQLLRELVQIPSVSGEEAAIAAYVEKIASQYGLP
                     VRRDDAMVAIELQSDKAGKGLALVSHLDTVPVGQGWTRPPFEPTVEGDRIYGRGANDA
                     KASCAAMIGAALDVLSAGGPASGRLLVVLGYGEETRDTSMPRAVPRLGPLDAAIIGEP
                     TSLDLAVAQRGLLMVDLVAHGDQCHAAYAAERGAQSSVLTLARDLVKLPTLLLDRPHP
                     LLGQPTITPTVLEAGVARNVTPPVAKAVLDLRTTPSWPHDELGSVLQAQLGSQVNVTS
                     SRLKPCETPPNSRLLAAMLSVRPFAQQFGSPTCSDWVFLRHLDAIKVGPGISRVSHTS
                     DEWVSIEQLTAARRFYAAVATQYLSPDFSTESLVFSERR"
     gene            4172..5587
                     /locus_tag="PKL17_08670"
     CDS             4172..5587
                     /locus_tag="PKL17_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNK44841.1"
                     /translation="MSAKAAPTTLWSPSATPDARALAFTIGDDPHWDSQLLRWDVIGS
                     LGHIAGLRSANILSPVDHQRLHNHLLSALSAVESGELFIAREHEDGHTAVELWLSQRD
                     PELGARLHTGRSRNDQIAVDLRLFMKSSLQKIVELAGQLATVLLDFAAQHRQTLWPGF
                     THQRRAMPSSLGLWSAALAEGLIDTIETHAALLGQIDRSPLGSAAGYGVPLSLDREAT
                     AQALGFAGLVQVVTAVQNGRGKLEAALLFFCTQLGHDLSRLCADVCLYSAEEFGFLIL
                     PADLATGSSLMPHKRNPDVFELGRAKMAALDGDLTQLRFLAGKLSSGYHRDFQLMKEP
                     LLRGVQRTMETLDVLQMAIPKLGVDKDRCLAALSGNLLVTDEVIRRVESGQPFRTAYR
                     SVADELKRDALTLPIADPADLIRRRRSTGGMGNLGLPKLRTRLRHCLRQNEKSQVQFR
                     SAIDRLLQPMPTHDSDTHNPK"
     gene            5592..6062
                     /locus_tag="PKL17_08675"
     CDS             5592..6062
                     /locus_tag="PKL17_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HNK44842.1"
                     /translation="MKSRRALRLRKLLELISSREMRTQEEVCQALLAEGWPVTQSSVS
                     RDIATLKLRKEDGVYVRQSPRSDKTQPNSLTVVLRESVLDFAPAGDALVVIHTPPSEA
                     SRVGAAIDRLAFSEVVGTIAGDDTVFVAVRDSAAQKRFLSHLHKQGEEDHPSTT"
     gene            6096..8258
                     /gene="fusA"
                     /locus_tag="PKL17_08680"
     CDS             6096..8258
                     /gene="fusA"
                     /locus_tag="PKL17_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNK44843.1"
                     /translation="MSSAPSPAPSESKAGAKSKKLDRVRNIGIIAHIDAGKTTVSERF
                     LFYSGRIHKVGDVDDGNTQMDWMPEERKRGITITAAATSFTWRNHEVHLIDTPGHVDF
                     TIEVERSLRVLDGAVVVMSAVDGVEPQTETVWHQADKFHVPRMVFVNKMDRVGANFES
                     VVEDVRKTLAAYPIPIQEPIGAEDTFQGAYDLIEQKKVTFSGDEDDPPEVSDPSDDDM
                     AVIGPAREKLIERVGEVDDAIAELFLTGSPVDAATLRAAIRRVTLARKAVPVLCGSAL
                     RNRGVQPLLDAVVDFLPAPTEVPAIVGQNPRTGEAESRPSDDKAPLCALAFKIFMLEG
                     RKAVYLRIYSGVLKAGDDVFNPRLGPPTKDDKGWKKGEAKEQKPLGRIEKIARLFTVH
                     AASREKIDQAGAGNIVLAVGLKDTATGDTLCDPRAQIVLEPIDTYQPVISQAIEASTS
                     ADAEKMLAALQKIADEDPTFQVRDDAETGQTLISGMGELHLEIARSRLESEYNVVART
                     GRPQVVYHETVLGQGEAEHLFERAAEDEQLFGRATVRVSPRARGEGLLVRSEVPPPSP
                     DWVPALSRALPGCIEAALSGIREAMGSGPKGNKLVDIEAVLVSVEPKLDVASDVGWRI
                     AGSRALIKACEAANTALLEPIMHLEVVVPDEFVGDVLGDLNQRRAQIHDVGVRGNRRC
                     VIAELPLRALFGYSTAVRSATQGRADFVMTFSKYDTWS"
     gene            complement(8272..9345)
                     /locus_tag="PKL17_08685"
     CDS             complement(8272..9345)
                     /locus_tag="PKL17_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK44844.1"
                     /translation="MGEDAEPAIASAAELPRLRAELAALSTEVQRLRALVAAVPDLII
                     RLHRDGTYLDFVGAKDIPVLISRSERIGRHVRDVLPKEVADAYLLATAAAQNTSQTQT
                     FRYQLPIGGKTGHYEARVVPSGPDEVLMVVRDVTTQHLAEEALRVEKECSEQILLNIL
                     PRPIVDELKRASGSISHRFESATVLFADIVGFTAAAASLPPAAVVETLNEIFTSFDSI
                     AERWDLEKIKTIGDAYMVVGGVPIARPNHAESIAEMALSMQEKICDFSFPGHPPLSLR
                     IGIATGPVVAGVIGMRKFIYDLWGDTVNVASRMESSGEPGRIQVAESTYEVLRDRYRF
                     EHRGEIPIKGKGMLSTYFLLGRV"
     gene            complement(9527..9601)
                     /locus_tag="PKL17_08690"
     tRNA            complement(9527..9601)
                     /locus_tag="PKL17_08690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9567..9569),aa:Gly,seq:gcc)
     gene            9782..11137
                     /locus_tag="PKL17_08695"
     CDS             9782..11137
                     /locus_tag="PKL17_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44845.1"
                     /translation="MHMSQDLTAILLPQPPRGPNPDTVVNIVAQNSPPTAWVRLSPLE
                     KLEVQQLEVMDGATGLPAVSPAMLVALSQNGGKAMFVHVNHPGKQALLHAFEDGIEVA
                     SYTGEPNEAFVAEFQKLVGHSVDDVVGADDGTRVGFGQAATRTAALVRGRLLMVPPGT
                     PTGLGSFVFHDRGFDAPSNLIVTGTDDEPAETTRAAFFAFDGNLIHQAFNQIPGGQLA
                     QVLGGAPEDVLGPLMELRDSTTKALSQHQTPPGQAKDHPAWHTHTFELLALSHAGVFG
                     GGDTLKFLDQKLLAILNIGDATPIIDADDAEELEEMPSVLDAMIDVLPCPKPPGGYGP
                     LFENIGPEEIGALVPWAKPGQPYDGAVFLLKPDRLLELARSFDANRLGQRLERFCRAL
                     YSAKMGDDVQEDAYLAWRGAWEQKSQKDIERLLLAWAEFRVVLEMAAQNRLNVGLAVY
                     G"
     gene            complement(11128..12696)
                     /locus_tag="PKL17_08700"
     CDS             complement(11128..12696)
                     /locus_tag="PKL17_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44846.1"
                     /translation="MFARLTVLLFCVFLLLGGREPPWADANVAYQTTQAIVDRGELSI
                     QLSAPAYFFVERNGKKYGFASLGTAIAQIPSYLAFRALRKLPGLAPGPWYAMASHLSS
                     ALWMSLAIALFARRAQRIGATATQAVALAFVLQLGTLCLVYGRSSYGEALQAFLLTWF
                     VDLTLLASEQKRKKTLLLCGLCAGMLLHAKLVYGAVLPVGLGLFLWAHRRDGLWANLR
                     ALGLPLCGALPGIALLLAHNWVKTGHPLRTGYQSGQLGLFDGDLLSGLFGLLLSPGRG
                     LVFYAPPLLLGVLGIHRAWQTERRLTVLVGLTAAVVTLISAKYPVWHGGYCYGPRYLV
                     PLIPMLLWLSVPWLSSLGQHQARRAGWIVALVGALGLWVNGLGSALYWDHYCRVLASV
                     QRQALVPGWSEDHHAFGYFVPQFSPIRGHFWLLRHTLRGDLDLPRDAPWLSVLGRPVD
                     LREEAARLRVDFWALDWLTGAPWTHKRYGFGLLGMLVAGVFWSARGLGARLRWDDRQT
                     AAARYGERAGGDQP"
     gene            12752..14083
                     /locus_tag="PKL17_08705"
     CDS             12752..14083
                     /locus_tag="PKL17_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK44847.1"
                     /translation="MLATLHLLLATAPMMNLAVNRPKMGVLPLFALLLFGLWVLLLRF
                     ALPGFGRKWTRAVLILGVLTVLMMVPRVLWRWAGAPPGIATLHRYLLGPMTVVQVAML
                     AGLFTAPLWVPLGRYIKRRLAREAAKAAPAESTSETPDPTTTHETAQAKELVTPSLPA
                     PMVLTRRKALQTLPWILPGGAMVAASYGVFVESSRIVLRKIKLAIPGLPKELSGFRIG
                     QVTDIHISRFQTQLHHLERGMELLARENLDLLCPTGDLCDEPRLHLDTLRIIRQVPAR
                     LGHIACAGNHELYLADPLTISRAYEKAQIQLLEEESVLVAGLRIAGIGFAHSGRVPRL
                     DQRLVPAQLETALKDRRQGEPTLLLAHHPHAFEHVGGHQVALQLSGHTHGGQVGLGGR
                     SLLEPVYPLIRGHYRGQDGVSQLFVSAGLGHWLPFRFGCPPEVVVIELVAV"
     gene            14226..15710
                     /locus_tag="PKL17_08710"
     CDS             14226..15710
                     /locus_tag="PKL17_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK44848.1"
                     /translation="MNQAPASSSEEATTESTPSRSPLQAAIAELIQPFRDIVKVSPAL
                     SLGTFVPYFLDGLSYLGMLTLLATYLAQNVALGDVWAGLLTGFLTGGITFAMFLLGST
                     SDRWGVRRSLIVSMAVLLLGRSLMALPEVLSLRPGLWNGLMLLNLLGMVVVIVGYGAF
                     QPATYAAVKEFSNEKTAPMSYALLYALQNLGAFVSGLLSPRIRNLSKGILPPNGITGV
                     FWFYAALTVLGLLLMIRYLPKKPQPADSELVARALSDAKHVFAQKQSRRKRLVTWLRE
                     HPLADPKFSFFIFVLIPVQTLFAHQWLTLPQYIERSYRAWPWVSENFETFSNLNPLLV
                     FLLTPLLTAATARRSVYAMMIWGTAIMGMPTVLLALGPSPLSLVAFMLIGAMGESLWQ
                     PRFLQYAAEIAPEGKTGLYMGVAQLPWFLTKLLTSLYSGWFLAHYCPQQGTLRTESMW
                     FYYWLIACVSPVGLILARKWVGSALSVRVIASDKATPAAEKTVA"
     gene            complement(15733..17913)
                     /locus_tag="PKL17_08715"
     CDS             complement(15733..17913)
                     /locus_tag="PKL17_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44849.1"
                     /translation="MQKRAFLRLALFSTAVSVGQLAILLFLTHALSPLGSRALVLSAG
                     LWLLGEALWLRVAQSPVAAARRTAQTQPALLPAFFVRSLLICGWAVVLSLLLLRMQLV
                     SPDLLAVLPWLGLSLGPLLAAVQVLALSRMDRRAVSVTIQSGQSAEPVAWFRQRLLRR
                     SGTVVLAGLATWAQLFALIFLVLKLWLSRHDWLFFRAFAPALAVGEWLWLISSIALLS
                     SIEKPLIGLAPGSPFGRREAVWLRVVHRLLLLPSRLSMAHALLTYAGFAAVVCWLVHG
                     DLLSFRQGEIFLGMVLIGESAAVLWLGLLARRSLRPLFPPASQHLSLRALSALRPPPL
                     SGQVLLLLSTVLLGLALLLLPRHWGSQLVLLLLFALILPGTALLLSLLHRKLLGRTFG
                     EVSLGTGDPGQQLGAWLVDAEHSVLGRALLSLSQELHAKLHAASDAQALLHREVEQRT
                     AELRDKNDALSKALAELAQTQDKLVESEKLASIGRLIANVTHEINNPVNAVLNSAAPL
                     SLLLSDLAQRLSDGEAMPLSDRQELVSDSAQMLQVIERGTLRTRDIVRSLHRYSVSDA
                     VMADSVQLADCLREAWTLCQGPGKQQVLVDWQLAELPPLSGHAGQLQQVLTNLLANAV
                     FALHERAQQDPSAPVPKLVLRTEQIGGEQIVAITDNGIGMTSEVRRRLFEPFFSTKDV
                     AHGTGLGLAIAHGIIRAHQGRISVESQPNQGTTFTICLPCERQG"
     gene            complement(17919..18506)
                     /gene="plsY"
                     /locus_tag="PKL17_08720"
     CDS             complement(17919..18506)
                     /gene="plsY"
                     /locus_tag="PKL17_08720"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNK44850.1"
                     /translation="MVILLAVLLSYLSGSVPYGLVFAKLRRVDLRAVGSGNIGATNAA
                     RALGKGFGALVLVLDALKATIPVLLALRYFADHPHKEWIAVAVALSAFVGHLLPVFAG
                     FAGGKGVATALGCFVALAPWPALLGLVSWAVLFGLSRISSVGSLGAVLLFPLWLYLCH
                     ASQPIYVLSGVFLLLILIRHRGNIARLWNKTEQRL"
     gene            complement(18914..20041)
                     /gene="cheB"
                     /locus_tag="PKL17_08725"
     CDS             complement(18914..20041)
                     /gene="cheB"
                     /locus_tag="PKL17_08725"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNK44851.1"
                     /translation="MSGRVLKVVIIDDQPVIRQALTALLRGEPDLQIVGQASDGEEGL
                     KVVFATQPDVITLDLEMPRLDGFAFLRLLMAKQPRPVVIVSSHATRESVFRALELGAV
                     EFVAKPSRIASDADLQPIRDDLLRKLRTVTQLSIRSVAERMRLHGQGVRLRTRSSEDL
                     LSGMRSLPEPALLPRFLVCIGASTGGPPAIATLLQALPSKLPISVLITQHMPSKFTAA
                     FVDRLRRTTEWSVREADPDLPVAPGEVLVAPASHSLCVYRDGGVLRVRKASEIQEPRG
                     GYVPSIDRMLSAAAEAMGPAVLAVILSGMSGDAVQGVQAVSRTAGRVLVESPQSAVIA
                     SMPEAVIQTGAVHEVAPLPKLASLIIRYVLSRPSISAAVDK"
     gene            complement(20038..20898)
                     /locus_tag="PKL17_08730"
     CDS             complement(20038..20898)
                     /locus_tag="PKL17_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNK44852.1"
                     /translation="MPSPQPGALSLDTPTFELLRKLIEARYGLVYTPETAYLLERRLA
                     SRVQAHGLSSFQEYYAQLTSPRIPHVMRDRELAEVFELLSTRETYFFRESYQLDAFRE
                     VLLPQLHAKRPRGNSLNIWSAGCASGEETYSLAVEIIESGLFEGWQVHVVGSDLSSQA
                     LQTAMHAVYGPSSFRQTDAARHNRYFRPLTGRWQVIESVRKLCTFHKLNLMDADFSSI
                     GGPFDAIFCRNVIIYFDRASRGSLIQRLADRLAPGGYLFLGHAESLLDANTPLGIAHL
                     GRELVYRKPA"
     gene            complement(20905..22137)
                     /locus_tag="PKL17_08735"
     CDS             complement(20905..22137)
                     /locus_tag="PKL17_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK44853.1"
                     /translation="MTLHNSAYDLAQLAAIFREGEEEARLSAVQKLNRQPEDETERRA
                     LLDLLIEVMGDDSWQVRKEAVASALSFTDTVLLAQKLVDAMSEPDNIGRRNAVIEGVM
                     KLGPVCIEPLLAALAQKPDYRKVLVDVLGTYGDTRVIPALGQSLLDEDPNVRAAAMEQ
                     LASFSSEEVVPALRAALRSPDMLVVLAALDGLNRQQVVLPIAEVLPLKEAVALRPAMM
                     TSLGYSKDSAAVPVLIDGLLDRARGAREAALVALYRLYQQLDDAGRKLVIDSAKALDE
                     MAVRSSLRALLEATPSVRQATAALLGWTGRREMLRPLVLALGDADKTVSETTGQAILQ
                     MGHDALSVLCDMLPTLDPRSRASAFQFFQRHSADIRDANLRSRIRSLLKYGTQDKNPA
                     TAQAAQQALTFYPSTSQD"
     gene            complement(22143..22709)
                     /locus_tag="PKL17_08740"
     CDS             complement(22143..22709)
                     /locus_tag="PKL17_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK44854.1"
                     /translation="MSRGRSIRGGFGLGNKTVERDKTRDEIVQLCGFRIGDEEYALDI
                     MRIKEIINPLAITRVPRAASFIEGVVELRGTILPVVDMRKRFDLPPLTAEQLEDYAVR
                     RARKYLIVPIEGRLVGMIVDRVSDVLRVSRDAIRPPPLLTQNENARYFAGVCHHRGRI
                     LMVLDLDAILSTTERISLAGLNQAAKGA"
     gene            complement(22706..23317)
                     /locus_tag="PKL17_08745"
     CDS             complement(22706..23317)
                     /locus_tag="PKL17_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK44855.1"
                     /translation="MAQPSGSPSNYDGSQRHPTGELRLARDVLTFHIAEEEYGIGIEH
                     IREIIKHRLVTEVPRVPGFVAGIIAVRGQVMPVLDLRQRLRMRAASLSAKSRILIVSR
                     PAASNENVSEEERERFGLVVDRVNEVVRIHEVDIEPPTVLSGKESDFVEGIGRIRAEV
                     DESARREARFGPGMITTEPQRIIILLDLVKVLSFEVMRSGRMF"
     gene            complement(23317..>23693)
                     /locus_tag="PKL17_08750"
     CDS             complement(23317..>23693)
                     /locus_tag="PKL17_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK44856.1"
                     /translation="SVMESLMLDYKQIRTIERREVITLRDQTLPLVRLEDIFSLQRPP
                     EMPEPQSGYVVVVALAQNRLGLLVDDLVGQQDIVIKSLGKTLQGIPGIAGATELGGQQ
                     TALVLDVRAVAEEAMPRISEVA"
BASE COUNT         4719 a         7613 c         7126 g         4235 t
ORIGIN      
        1 gatgcgtatg gcggacatgc ggtggcgtcg acgggccact gtcatcccaa agtggtccag
       61 gcgatttcgg cccaggccgc gcagcttttg ttttattcga cggcactccc gcatccgggg
      121 cgcgagcggc tcgccgaagc catcgtgtcg cactgcccca gcgcgctgcg cagagtgttc
      181 ttggtcaatt ccggggccga agcgaacgaa aacgcgctgc atctggcgcg aaaaatcacc
      241 gggcgacagc acttcgtgtc gatcgagggt ggttggcacg gacgcaccgc agcgacgttg
      301 gcagtgaccg acggcgaaaa atacatggcg ggcgcgcggc gggccggcat tccgctgtct
      361 gtgaaagtgc cgtggaacaa tccggatgcg ctgaaacagg cggtcagtga atcgattgcc
      421 ggcgtgattt tggagccagt ccaaggtctg accggcgccc gcgatttgcc acctgcgctg
      481 ttgcaagcgg ccaggcaggc ttgcgacaag cacggtgcgc tgctgatttt cgacgaagtg
      541 cagtgtggcg tcggacgctg tggcgcattt accgccgccg agtcgtttgg cgtcaccccc
      601 gatgtgctga cgctggccaa aggtctggcg tcggggctgc cgatttcagc ggtggtgaca
      661 accgacgcgg tggcccagca cataaagcaa ggcgacttgg gcagcacgtt tggcggcggt
      721 ccggtgccgt gtgcagcggc gctcgcgaca ctgcaagtca tcgacgaaga acggctgatc
      781 gaaaatgcaa tggccgttgg tgatttcttg gccaaagggg cgctttcgct tggccaagac
      841 aaagtggtgg cgacgcaggg acgcggactt ttgctcgggc tgcggcttgc aaaaccggcg
      901 gcgcaggtcc aacacgcgct gcttgcgcat cacatcgtaa gtggcacgtc gtcggaccca
      961 caggtgctcc ggctgatgcc gccgctgacg ttttcaaaat ccgaagcaga gcttgtcctg
     1021 tcggcgcttt cggaggtgct gtaaatggtc gcttcgacgc aaatcccagc gcggaaaaag
     1081 cgcgattttt tggacatggc cgacctggca ccgcttgcgc tggccaagct gctcgatttg
     1141 gcagcgcagc tacgacgcgg cgacgcaaaa cacaaagacg cactcgcaca aaaagtgctc
     1201 gcgatggtgt ttctcgaccc gagcctgcgc acccgcacca gcttcgaggt tgcgatgttt
     1261 ctgcacggcg gccacggcgt cgtcctcgaa ccgggtcgca caagctgggc gtgggaaacc
     1321 cgcaaaggcg tcgtcatgga cggcaccacc gtcgaacaca tcgctgaagc cgcgcgcgtc
     1381 ttgggacggt acggcgacgc ggtggccctg cgcgcattcc cgcgaggcgg cgcttggtcc
     1441 gaagagcgcg aagatcgcat catccacgac tttgccgcct actgcgaaaa gccggtcatc
     1501 aacatggaat cggtgcgccg ccatccgtgt cagggtctgg ccgatgcact gacgattcgc
     1561 gaccgactgc ccaatcccaa aggccgtcgc tgcgtgctga tgtgggctta tcatcccaag
     1621 ccgctgccga cggcagttcc ggccagcgcg gcgattgcag cagccagcca aggcatggcg
     1681 cttcagattg tccatccgcc cggttacgag ctggaccaag acgacatggc gacgattcgc
     1741 agcgtggcgt cccaaaacgg cggcagcgtc gatgtgtcgc acgatctcga tgggagcctc
     1801 catgacgccg acgtggtgta cgtcaagtcg tggggcgcga tcagtcactt tggtcgaccc
     1861 agcgaagaag ccgcgctgcg cagtccttat cgcgactggc gaatgaccaa agaccgcagc
     1921 gaaatgcttc atccggacgg gtttgtcatg cactgcctgc cggtgcggcg aaacattgaa
     1981 atagacgatg cggtgctcga cgggccgcgg tctgcggtcg tcgatcaggc ggaaaaccgg
     2041 cttcatgccc agcgcgccct gctcatcgat cttgtctcgg acctggcgct gtaagagcga
     2101 aagaaaggaa ctcctcgtgt caatcgagcc agccaaaggc attgccggtc tcaaaggggc
     2161 gctgcgttat gtgcgcgcct accgtgacca gatttttgtg atcaagctcg gcggtgaagt
     2221 cctggaacgg gccgaatcgc tgtccggagt ggcggcgcag ctttcgcttt tgtcgtcgct
     2281 gggtgtccat ctggtgatcg tccacggcgg cggtccccag gccagccagc tcacgcggcg
     2341 tctcggtggt gaacccaaga tgatcgcggg acgacgaatc accgatgaca ccgcgctgtc
     2401 ggcagtcgtg atggcggtgg ccggtcagct caatgtgcag gtgctgtcgg cactgcgggc
     2461 ccaccatgtg cgcgccgtcg gtctttcggg tgtcgatgcc gaactggtca ctgcccacaa
     2521 gcgcccgccc gttgaagtgg tcgatgacag tggccagacc caaaaagtcg acttcggcca
     2581 cgtcggcgac attgatcgcg tcgatccgcg ggtcatcgaa acgctgctgg catcgggtta
     2641 tgtgcccgtg atctcgtgtc tggccggcga caaccaaggc aacatctaca acatcaacgc
     2701 cgacacgctg gccgaaagct tggccgtcgc gctgcgggcg cagaagctga ttttcatgac
     2761 gtctgcgccg ggtgtgctgc gcgatcgcaa cgatccatcg acgctggtga cgtttgccga
     2821 tcccgatgat ctggccagcc tgctgtcgag cggttcgatt gccggcggca tgcgacccaa
     2881 agtcgaagcc tgcatccgtg cagcgacggg cggcgtcgaa cgaacccaca tcatcgacgg
     2941 cggcgcgccc gactcgctcc tgttcgaggt cttcaccggc gcgggctgcg gcacgatgat
     3001 cgtcggacgc aaagaaaaag cgacttatct cggagtcgat cttgccgcac tccttggcta
     3061 gcacgacggc gcggctcgat gaagtgcagc ttctgcgcga gcttgtgcag attccgtctg
     3121 tgtccggcga agaagcagcg attgcggcct acgttgagaa gattgcttcc caatacggcc
     3181 tgccggtgcg tcgcgacgat gcaatggtcg ccatcgagct gcagagcgac aaagccggca
     3241 aaggccttgc gctcgtttca cacctggaca ccgttcccgt cggacaaggc tggacgcgac
     3301 cgccttttga gcccaccgtc gagggtgaca ggatctacgg tcgcggcgca aacgacgcca
     3361 aagcaagctg cgcagcgatg atcggagcgg cgctcgatgt gctttctgcg ggcggtccag
     3421 cgtcgggacg cctgcttgtc gtgctgggtt atggcgaaga aacgcgcgac accagcatgc
     3481 cgcgggcggt gccacggctc ggaccgctcg acgcggcgat catcggtgag cccacgtcac
     3541 tggatttggc ggtcgcacag cgagggcttt tgatggttga tctcgtcgca cacggggatc
     3601 aatgccacgc ggcctatgca gcagagcgcg gcgcacagtc atcggtgctg acgctggccc
     3661 gcgatctggt caagctgcca acgctgcttc ttgacagacc gcatccactg cttggacaac
     3721 cgacgatcac cccgacagtg ctggaagccg gcgtggcccg caatgtcacc ccgcccgtcg
     3781 ccaaagccgt gctcgatctg cgcaccacac caagctggcc ccacgacgag ctgggatcgg
     3841 ttttgcaagc gcagcttggc agccaagtca acgtgacctc gtcgcggcta aagccgtgtg
     3901 aaacgccgcc aaactcgcgc ctcctcgccg ccatgctatc ggtcaggccc tttgcgcagc
     3961 agtttggcag cccgacgtgt tcggactggg tgtttttgcg ccatctcgac gccatcaagg
     4021 tcggccccgg cataagccgc gtttcccaca ccagcgacga gtgggtttcc atcgaacagc
     4081 tgaccgcggc acgccgtttt tacgccgccg tcgccaccca gtacctatcc cccgactttt
     4141 cgacggaatc tttggttttt tcggagcgac gatgagcgcg aaagctgccc ctaccacgct
     4201 ttggtccccg tcggccaccc cagatgcgcg ggccttggct tttaccatcg gcgacgatcc
     4261 gcactgggac agccagcttc tgcgctggga cgtgatcggc agcctgggtc acatcgccgg
     4321 gctgcgcagt gccaacatct taagtcccgt cgaccaccag cgtctgcaca accacttgct
     4381 gagcgcactt tcggcggtcg aaagcgggga gcttttcatc gcgcgcgaac atgaagacgg
     4441 ccacaccgcc gtcgagcttt ggctgtcgca gcgcgatccg gagctgggag cgcgtctgca
     4501 caccggacgg tcgcgcaatg accaaattgc cgtcgatctg cggcttttta tgaagtcgtc
     4561 gctgcaaaag attgtcgaac tggcgggaca gcttgcgacg gtgctgctcg atttcgccgc
     4621 ccaacatcgc cagacgctgt ggccgggttt tacccatcag cgacgggcca tgccatcgtc
     4681 tttgggactt tggtcggcag ctttggccga aggactgatt gacaccatcg agacgcatgc
     4741 ggcgctgctc ggacagatcg atcgctcgcc gctcggtagc gctgccggct atggcgtgcc
     4801 gctttcactc gaccgcgagg cgacggcgca agcgctcggt tttgccggtc ttgtgcaggt
     4861 ggtgacggcg gtgcaaaacg gacgcggcaa gctcgaagca gcgctgcttt ttttctgtac
     4921 ccaactgggt cacgacctgt cgcgactgtg cgccgatgtc tgcctgtatt cggcggaaga
     4981 gtttggcttt ctgatcctgc ctgccgatct ggcgacgggt tcgagcctga tgccgcacaa
     5041 gcgaaacccc gatgtgttcg agctgggacg cgcgaagatg gcggcgctcg acggggattt
     5101 gacccagctg cgctttttgg cgggcaagct gtcgtcgggc tatcaccgcg actttcagct
     5161 gatgaaagag ccgcttttgc gcggggtgca aaggacgatg gaaacgctgg atgttttgca
     5221 gatggcgatt cccaagctcg gcgtcgacaa agaccgctgc ttggctgcgc tgtctggcaa
     5281 cttgctggtc acagatgaag tgatccgccg cgtcgaatcg ggccagccct tccgcaccgc
     5341 ctatcgcagc gtcgccgacg agctaaagcg cgatgccttg acgctgccga tcgctgatcc
     5401 tgccgatctg attcgccgcc gccgctcgac gggtgggatg ggcaacctgg gactgcccaa
     5461 gctgcggact cgactgcgcc actgcctgcg tcaaaacgaa aaatcccagg tccagtttcg
     5521 ctccgcgatc gaccggctgt tgcagccgat gccgacgcac gattccgaca cccacaaccc
     5581 caagtgagac gatgaaaagc cgacgcgcgc tccgtctacg caagctgctt gaactgattt
     5641 cttcgcgtga aatgcggacc caggaagaag tctgtcaggc gcttctcgcc gaaggatggc
     5701 cggtcactca gtcgtcggtg agtcgcgaca ttgcgacgct caagctccgc aaagaagacg
     5761 gcgtgtatgt tcgccaaagt ccacgcagcg acaaaacgca gccgaattcc ctgacggtgg
     5821 tgctgcgcga atcggtgctt gattttgccc cggctggcga cgcgctggtg gtcattcaca
     5881 ccccgccgag tgaagccagt cgagtcggcg ccgccattga tcgcctggcc ttttccgaag
     5941 tcgtgggcac catcgctggc gacgacaccg tctttgtggc cgttcgcgac agcgctgcgc
     6001 aaaaacgatt tttgtcacac cttcacaagc aaggcgaaga agaccaccca tcgaccacct
     6061 agccagcacg cacagttgtc tctacactgc gcgccatgtc ttcggcccca tctccggctc
     6121 cgagcgagtc caaagccggc gccaagagca agaagctcga tcgcgtccgc aacatcggaa
     6181 tcatcgccca catcgacgct ggcaaaacca cggtcagcga acgttttctg ttttattcag
     6241 gccgcatcca caaggtcggc gatgtcgacg acggcaacac ccaaatggac tggatgcccg
     6301 aggagcgcaa gcgcggcatc accatcaccg ccgcggcgac gtcgtttacc tggcgcaacc
     6361 acgaagtgca cctcatcgac acccccggcc acgtcgactt caccattgaa gtcgagcgct
     6421 cgctgcgggt tctcgacggt gcagtcgtcg tcatgtccgc cgtcgatggc gtcgaaccac
     6481 agacggaaac cgtctggcac caagccgaca agtttcacgt cccgcgcatg gtcttcgtca
     6541 acaagatgga ccgggtcggt gccaactttg aatccgtcgt cgaggatgtc cgcaagacgc
     6601 tcgctgcgta cccgattccg attcaagagc cgatcggcgc cgaagacacg ttccagggcg
     6661 cttacgatct gatcgaacag aaaaaagtca cgttcagcgg cgatgaagac gatccgcccg
     6721 aagtcagcga tcccagcgac gatgacatgg cggtgattgg cccagcgcgc gaaaagctga
     6781 tcgagcgcgt cggtgaagtc gatgatgcga tagccgagct gtttctgact ggatcgcccg
     6841 ttgatgcggc gacgctgcgg gcggcgattc gtcgcgtcac cctggcgcgc aaagccgtgc
     6901 cggtgctgtg tggatcggca ctgcgcaacc gcggtgtgca gccgctgctt gatgcggtcg
     6961 tcgattttct gccggcgcca accgaagtcc cggcgatcgt cggacaaaat ccgcgcaccg
     7021 gcgaagccga aagtcgaccg tcggacgaca aagcgccgct gtgtgcactc gcgttcaaaa
     7081 tcttcatgct cgagggacgc aaggcggtct acctgcggat ttattcgggc gtgctcaaag
     7141 cgggcgacga tgtcttcaat ccgcggctcg gaccgccgac caaagacgac aagggctgga
     7201 aaaaaggcga agccaaagaa caaaagccgc ttggtcgaat cgaaaaaatt gcccgcctgt
     7261 ttaccgtcca cgccgccagc cgcgaaaaaa tcgatcaagc cggtgcggga aacatcgtgc
     7321 tcgcggtcgg gctcaaggac acggcgacgg gcgatacgct atgcgatcca cgggcgcaga
     7381 tcgtgctgga accgattgat acctatcagc cggtgatctc gcaggcgatc gaagcgtcga
     7441 cgagtgccga tgccgaaaag atgctggcgg cactgcaaaa gattgccgac gaagatccta
     7501 cgttccaggt ccgcgacgat gccgaaacgg gtcagacgct gatttcgggc atgggtgaac
     7561 tgcatctgga aatcgcgcgc tcgcgtctgg aatcggaata caacgtcgtc gcgcgtaccg
     7621 gacgtccgca ggtcgtctat cacgaaaccg tgctgggcca aggcgaagcc gaacacctgt
     7681 tcgagcgggc cgccgaagac gaacagctgt ttggtcgtgc gacggtgcgg gtgtcgccac
     7741 gggcccgtgg ggaaggactg ctcgtccgca gcgaagtgcc accgccgtcg cccgattggg
     7801 tgcctgcgct gtctcgcgcc ctgcccggct gcatcgaagc tgcgctcagc ggcattcgcg
     7861 aagcgatggg atcaggacca aaaggcaaca agctcgtcga cattgaagcc gtgcttgtgt
     7921 ccgtcgagcc caagctcgat gtggcgtccg acgtcggctg gcggattgcc ggttcgcgcg
     7981 cgctcatcaa agcctgcgaa gctgccaaca ccgcgctgct cgagccgatc atgcacctgg
     8041 aagtcgtggt ccccgacgag tttgtcggcg atgtgctcgg ggatctcaat cagcgccggg
     8101 cgcaaatcca cgatgtcggc gtccgcggca accgtcgctg tgtgatagcc gagctgccgc
     8161 tgcgagcgct gttcggctac tcgacggcgg tgcgttcggc gacgcaggga cgcgctgact
     8221 ttgtgatgac gttttcgaaa tacgacacct ggtcgtaacc aacacccacc gctagacccg
     8281 cccgagcaga aaatacgtcg acaacatccc tttgcctttg atcggaatct cgccgcggtg
     8341 ctcgaagcga tagcggtcgc gcagcacttc atacgtcgat tcggcaacct gaatccgacc
     8401 cggctctccc gacgattcca tccggctggc gacattcacc gtgtcgcccc acaagtcata
     8461 aatgaacttg cgcatgccaa tcacaccggc gacgaccgga cctgtggcga taccgatgcg
     8521 gagcgacagc ggcgggtgcc ccggaaacga aaaatcgcag attttttcct gcatcgacag
     8581 cgccatctcg gcaatcgact cggcgtgatt cggtcgcgca atcggcacgc caccgacgac
     8641 catgtaggca tcgccgatcg tcttgatctt ttccaagtcc cagcgctcgg cgatcgagtc
     8701 aaacgacgta aagatttcgt tcagcgtctc gaccactgct gccggcggaa gactcgctgc
     8761 cgcggcggta aatcccacga tgtccgcaaa caacaccgtc gccgattcaa agcgatgcga
     8821 gatgctgccg ctcgctcgtt tcagctcgtc gacaatcggc ctcggcagga tgttcagaag
     8881 aatctgctcg gaacactctt tttcgacccg cagcgcttct tccgccaggt gctgggtcgt
     8941 cacgtcgcgc accaccatca gcacttcatc cggaccgctc ggcaccaccc gcgcttcgta
     9001 atgcccggtc ttgccgccaa tcggcagctg atagcgaaac gtctgggtct gcgaagtgtt
     9061 ttgcgccgct gccgtcgcca gcaggtacgc atccgccact tctttgggca gcacgtcgcg
     9121 cacatgtcgg ccaatccgct cgctgcgtga gatcagcacc gggatgtctt tggcccccac
     9181 aaaatcgagg tacgtaccgt cgcggtgcag ccgaatgatc aagtccggaa ccgccgcgac
     9241 cagcgcccgc agccgctgca cttccgtcga tagcgctgca agttcggccc gcagccgcgg
     9301 tagctcggca gcacttgcga ttgcgggttc ggcgtcctcg cccataacgc catggtacga
     9361 gcgcggccag aaagcaggtg caaaaagcaa acgatcgaac catcttcgat cgcctagaag
     9421 gagcgctcgc cgcacagacc cgtgcccgcg aatcacagcg gcccaaacag ctgggcccaa
     9481 aatgaaaaaa gccgcccaat cggagcggct tattcatcga aaaatatgga gcgggagaag
     9541 ggatttgaac cctcgacgtc caccttggca aggtggcact ctaccactga gttactcccg
     9601 cgagagacgg aaggcagtct atcttggcca ctttgtaagt caacagaatt ttttgaactt
     9661 ttttcagccg cgcaagcccg cgctgccacc aaaaaaaccg caggacgtac acccgaaacg
     9721 acgcgcgccg cagcggtcac gcttgcaact tggcaaagaa ccgaggtggg aataacctac
     9781 ggtgcacatg tctcaagacc tgaccgcaat ccttctgccg cagccaccgc gcggcccaaa
     9841 ccctgacacc gttgtgaata tcgtcgccca gaactcgccg cccacggcct gggtccgcct
     9901 gtcgccgctc gaaaagctgg aagtccaaca gctcgaggtg atggacggag caactggtct
     9961 cccggcggtt tctccggcga tgctcgtcgc gctgtcgcaa aacggcggca aggcgatgtt
    10021 tgttcacgta aaccatcccg gcaagcaagc gctgctgcat gcgttcgagg atggcatcga
    10081 agtcgcaagc tataccggcg agcccaacga agcgtttgtg gcggagttcc agaagctggt
    10141 cgggcactcg gtcgacgatg tggtcggcgc cgatgatgga acccgggttg gctttggcca
    10201 ggctgcgacg cgcaccgcgg cgcttgtgcg gggacggctg ctgatggttc cgccgggtac
    10261 gccgacggga cttggttcgt ttgtgttcca cgatcgcggc tttgacgcac cgtcgaacct
    10321 gattgtgacc ggcacggacg atgaacccgc cgaaacgacc cgcgcggcgt tctttgcttt
    10381 cgacggcaac ctgatccacc aggcgttcaa ccaaatcccc ggtggccagc ttgcccaagt
    10441 gctgggcggc gcgcccgaag atgtgctcgg accgcttatg gagctgcgcg attcgacgac
    10501 caaggcgctc agccaacacc agacgccgcc gggacaagcc aaagatcacc ccgcgtggca
    10561 cacccacacg tttgaactgt tggcgctgtc gcacgccggt gtcttcggcg gaggcgatac
    10621 gctcaagttc ctcgatcaga agctactggc catcctgaac attggcgacg cgacgccgat
    10681 cattgatgcc gacgacgccg aagaactcga agagatgccg agcgtgctcg atgcgatgat
    10741 cgacgtgctg ccctgcccca aaccaccggg aggctacggg ccgctgttcg aaaacattgg
    10801 tcccgaagaa atcggtgcgc tggtgccgtg ggccaagccc ggacagccct acgatggtgc
    10861 ggtgtttctg ctcaagccgg atcggctgct ggagttggcg cgcagcttcg acgccaatcg
    10921 gctcggtcag cggcttgaac gattctgccg cgcgctctac agcgccaaga tgggcgacga
    10981 cgtgcaggaa gacgcttacc tggcctggcg cggcgcgtgg gaacagaaga gccaaaaaga
    11041 catcgaacgg ctgctgctcg cgtgggcgga gttccgcgtc gtgctcgaaa tggcggcgca
    11101 gaaccggctc aacgtcggtc tcgcggtcta cggctgatcg cccccggccc gttcaccgta
    11161 tcgggccgct gccgtttgtc tgtcgtccca ccgcagccgc gcgcctagac cacgcgccga
    11221 ccaaaaaact cccgcaacca acattccaag cagcccaaag ccatatcgct tgtgcgtcca
    11281 cggtgccccc gtgagccagt cgagcgccca gaagtccacc cgcagacgcg ccgcttcttc
    11341 ccgcagatcg accggccttc cgagcaccga aagccacggc gcatcacgcg gcaagtccaa
    11401 gtcgccgcgc agcgtgtggc gcagcaacca aaaatgtccg cgaatcggcg aaaactgcgg
    11461 cacaaagtac ccgaaggcgt ggtggtcttc gctccagccc ggcacgagcg cctgcctttg
    11521 caccgacgcc agcacccggc agtaatgatc ccaatacagc gcactgccca ggccgtttac
    11581 ccaaagcccc agcgccccca caagcgccac aatccatccc gcccgccgcg cctggtgttg
    11641 ccccagcgat gaaagccacg gcaccgaaag ccacagcagc atcggaatca gcggcacaag
    11701 atagcgcggc ccgtagcagt agccgccgtg ccagaccgga tacttggccg aaatcagcgt
    11761 caccactgcc gccgtaagcc ccacaagcac agtcagccgc cgctcggttt gccacgcccg
    11821 atggattccc agcacgccca acagaagcgg cggcgcgtaa aacacaagtc ctcgtcccgg
    11881 cgaaagcaac agcccaaaca gaccggacaa caggtcgcca tcaaacagac caagctgtcc
    11941 cgactgatag cccgtgcgca gcggatgccc ggtcttcacc cagttgtgcg caagcaaaag
    12001 cgcaattcca ggcagcgcac cacaaagtgg cagcccgagc gcgcgcaagt tggcccaaag
    12061 tccatccctg cggtgcgccc acaaaaacaa gccgagccca acgggcaaca cggcgccgta
    12121 gaccagcttg gcgtgcagca acatcccggc gcacaaccca cacagaagca gcgtcttttt
    12181 gcgcttctgt tcacttgcca acagcgtcaa atcgacaaac caggtgagca aaaacgcctg
    12241 aagcgcttcg ccatacgagc tgcgtccata gaccaaacac agcgtcccaa gctgaagcac
    12301 aaacgccaaa gccaccgcct gtgtggccgt cgcgccgatc cgctgagccc gccgcgcaaa
    12361 cagcgcaatc gccagcgaca tccacaacgc cgacgaaaga tgggacgcca tcgcatacca
    12421 cggacccgga gcaagtcccg gcagcttgcg cagcgcccga aacgcaaggt agctggggat
    12481 ttgagcgatg gccgtcccaa gcgaagcaaa gccgtacttt ttgccgttgc gctcaacgaa
    12541 aaagtaagca ggcgccgaaa gctgaatcga cagctcgcct cgatcgacga tcgcttgcgt
    12601 ggtctgatac gcgacgttgg catcggccca cggtggttcc cgtcccccaa gcaaaaggaa
    12661 cacgcaaaac agcagcaccg tcagacgggc aaacatgtgc ggagggtatc acttttgcga
    12721 cagcctctgc ttgcggcata gtgaaggttc gatgcttgcg actctgcatc tgctgcttgc
    12781 gactgcaccg atgatgaatc tggcggtgaa ccgaccgaaa atgggcgtgc tgccgctttt
    12841 cgcgctgctg ctctttgggc tgtgggtctt gctgctgcgc tttgcgctgc ccggttttgg
    12901 tcgcaagtgg acccgcgcgg tgttgatcct cggcgtgctg acggtgctga tgatggtgcc
    12961 gcgggtgctg tggcgctggg ctggggcacc gccgggcatt gcgacgctgc atcggtatct
    13021 gctcggaccg atgacggtgg tgcaagtggc gatgctggcg ggtcttttta ccgcgccttt
    13081 gtgggtgccg ctcgggcgct acatcaaacg gcgactggcc agggaagccg ccaaggcagc
    13141 tccggcggaa tcgacttctg aaacgccaga cccgacgacg acccacgaaa cagcccaggc
    13201 caaagaactt gtcacgccga gcctgccagc gcccatggtg ctgacccgac gcaaagcgct
    13261 gcaaaccctg ccgtggattt tgcccggcgg cgccatggtg gccgccagct acggcgtctt
    13321 tgtcgagtcg tctcgcatcg tgctgcgcaa aatcaagctg gcgattcccg gcctgcccaa
    13381 agagctgtcg ggctttcgca tcggccaagt cacggacatt cacatctcac gctttcagac
    13441 ccagctccat cacctggagc gtggcatgga gcttctggcc cgcgaaaacc tcgacctttt
    13501 gtgtccgacc ggcgatctct gcgacgaacc ccggctgcat ctggatacgc tgcggatcat
    13561 caggcaagtg ccggcgcggc tgggtcacat cgcctgcgca ggcaaccacg agctgtatct
    13621 ggccgatccg ctgacgattt cgcgggcgta tgaaaaagcg cagattcagc tgctggaaga
    13681 agagtcggtg ctggttgcgg gactgcggat tgccggaatt ggctttgcgc actccggtcg
    13741 ggtgccgcgg ctggatcagc ggcttgtgcc ggcgcagctt gaaaccgcgc tgaaagaccg
    13801 tcgccaaggc gagccgacgc ttcttttggc gcatcacccg cacgcttttg aacacgtcgg
    13861 cggtcatcaa gttgcgctcc agctttcggg ccacacgcac ggcggccaag tcggtctggg
    13921 cggcaggtcg ctgctcgagc cagtctatcc gcttatccgc ggtcactatc gcggtcaaga
    13981 tggcgtgtcc cagctttttg tgtctgcggg tcttggccac tggttgccgt tccgctttgg
    14041 ctgcccaccg gaagtcgtcg tcatcgagct ggtcgcggtc tgaccgacgc gacagccaca
    14101 tccccggcac tgcataacga actgccttgg gccttgcgct tgggcccccg tgatcgtttg
    14161 cgcagtttct gattgccagc ggaagagctg ggcaataaat aatgcgcgcg gatgttgtca
    14221 ccgcgatgaa ccaagcgccc gcctcctcgt cggaagaagc caccacagag tccacgccat
    14281 cgcgaagccc gcttcaggct gccatcgccg agctgattca gccctttcgc gacattgtga
    14341 aagtcagtcc ggcgctgtcg ctgggaactt tcgttccgta ttttctcgat gggctgtcct
    14401 atctgggaat gctgacgctg ctcgcgacgt atctggcaca gaacgtggcg cttggggatg
    14461 tgtgggcggg cttattgacc gggtttctga ccggcggaat cacgtttgcg atgttcctgc
    14521 tcggatcgac aagtgaccgc tggggcgtgc ggcgttcgct gattgtgtcg atggcggtgc
    14581 tgctcctggg tcgctcgctg atggcgctgc ccgaagtgct ttcgctaagg cccggtctgt
    14641 ggaacgggct gatgctgctg aacctgctcg gcatggtggt tgtgatcgtc ggctacggcg
    14701 cgtttcagcc ggcgacctat gcggcggtca aagagttttc aaacgaaaag accgcgccga
    14761 tgagctacgc gctgctctat gcgctgcaaa acctgggggc gttcgtttcg gggcttttgt
    14821 cgccgcgcat ccgcaacctg agcaaaggca tcctgccgcc aaacggcatc accggcgtgt
    14881 tctggtttta tgccgcgctg acggttttgg ggctgctttt gatgattcgt tatctgccca
    14941 agaagccgca gccggcggac tcggagctgg tggcgcgagc gctcagcgat gccaagcacg
    15001 tttttgcgca gaagcagagc cgccgcaaac gccttgtgac ctggctgcgc gaacatccgc
    15061 tcgccgatcc caagttcagc ttttttatct tcgtgctgat tccggtgcag acgctgtttg
    15121 cgcaccagtg gctcacgctg ccgcagtaca tcgagcgctc ataccgagcg tggccctggg
    15181 tcagtgaaaa cttcgagacg ttttccaacc taaacccgct gctggtgttt ttgctgacgc
    15241 cgcttttgac cgcggccacc gcacgacgca gcgtctacgc gatgatgatc tggggcaccg
    15301 ctatcatggg aatgccgacg gtgctgcttg cgctggggcc cagtcctcta tcgcttgtgg
    15361 cgtttatgct gattggcgcg atgggtgaat cgctgtggca accgcgcttt ttgcagtacg
    15421 cggcagagat tgcgccagaa ggaaaaaccg gcctgtacat gggcgtcgcg cagctgccat
    15481 ggtttttgac caagcttctg accagtctgt attcaggctg gtttttggcc cactactgtc
    15541 cgcagcaggg cacgctgcgc accgagtcga tgtggtttta ctactggctg attgcctgcg
    15601 tttcgccggt cgggctgatc ttggcgcgca agtgggtcgg cagtgcgctg tctgtccgcg
    15661 tcatcgccag cgacaaagcg acgcccgcag ccgaaaagac cgtcgcataa agcccggccc
    15721 ctcgcgggtt tcctacccct gtctttcgca gggaagacag atggtgaacg tcgtgccctg
    15781 gtttggctgc gattcgacgc tgatgcggcc ttgatgggcg cgaatgatgc cgtgcgcgat
    15841 ggccagtcca agtccggtgc catgggcgac gtctttggtg gaaaaaaacg gctcgaacaa
    15901 gcggcggcgc acttcacttg tcatgccgat gccgttgtcg gtgatggcca cgatctgctc
    15961 gccgccgatc tgttcggtac gcagcaccag cttgggcaca ggcgcgctcg gatcttgttg
    16021 tgcccgttcg tgcagcgcaa acaccgcgtt ggccagcaga ttggtcagca cctgctgtag
    16081 ctgtccggca tgaccggaaa gtggcggcag ctcggcaagc tgccaatcga ccaagacctg
    16141 ctgtttgccc ggcccttggc acagtgtcca cgcttcacgc aggcagtcgg caagctgcac
    16201 cgaatccgcc atcaccgcgt cgcttaccga atagcgatgc aagctccgca cgatgtcgcg
    16261 ggttcgcagg gtgccgcgct cgatgacttg caacatctgg gcgctgtcgg acacaagctc
    16321 ttgccggtcg gacagcggca tcgcttcccc atccgaaagc cgctgcgcca ggtccgaaag
    16381 caaaagcgaa agcggcgccg cgctgttgag cactgcgttg accgggttgt tgatttcgtg
    16441 cgtgacgttg gcaatcaagc gaccaatcga cgcaagcttt tcggactcca ccagcttgtc
    16501 ctgggtctgt gctagctcgg ccagcgcttt cgacagcgca tcgtttttgt cacgcagctc
    16561 ggcggtccgc tgctcgacct cgcggtgcag cagcgcctga gcatccgacg ccgcgtgtag
    16621 cttggcatgt agctcttgcg acagcgacaa aagcgcccgc ccaagtacgc tgtgctccgc
    16681 atcgaccagc cacgcgccaa gctgctgccc cggatcgccc gtgccgagcg acacctcgcc
    16741 aaacgtccgt ccgagcagct tgcgatgcaa aagcgacaga agcagcgctg tccccggcaa
    16801 aatcagcgca aacaacaaaa gcagcacaag ctgcgagccc cagtgccgtg gcaaaagcaa
    16861 aagcgcgagc cccaagagca ccgtgctaag cagcagcagc acctgccccg acagcggtgg
    16921 gggccgcagc gccgacaatg cccgcagcga tagatgctgg ctcgctgggg gaaacaaagg
    16981 ccgcagactg cggcgcgcca aaagcccaag ccacagcaca gcggcgcttt cgccaatcag
    17041 caccatgcca aggaaaatct ccccctgtcg gaagctcagc agatccccgt gcacaagcca
    17101 gcagaccacg gcagcaaacc ccgcataggt cagaagcgca tgtgccatcg acagccggga
    17161 cgggagcagg agcagccgat gcaccacccg cagccacacc gcttcgcgcc gcccaaacgg
    17221 agagcctggc gcaagtccaa tcagcggctt ttcaatgctc gacagaagtg caatcgacga
    17281 aatcagccac agccattccc ccaccgccag cgcgggtgca aaggcgcgga aaaacagcca
    17341 gtcatgccgg gaaagccaca gcttgagcac caaaaagatc agggcaaaaa gctgggccca
    17401 cgtcgcaagt ccagccagca caaccgtccc tgaccgccgc agaagccgct gccgaaacca
    17461 cgccaccggc tcggcggact gtccgctttg gatcgtgacc gacaccgcgc gccgatccat
    17521 gcgcgacagc gccagcacct gcaccgcggc aagcagcggc cccaaagaca gcccaagcca
    17581 cggcagcacc gccaaaagat caggcgatac aagctgcatc cgcagaagca agagcgacaa
    17641 caccaccgcc cagccacaga tgagcaggct gcgcacaaaa aacgctggca agagcgccgg
    17701 ctgggtctgc gccgtccgcc gcgccgcggc cactggactt tgggcaacac gcagccacag
    17761 cgcctcgcca agcagccaca gccccgccga aagcaccagc gcccttgagc caagcggcga
    17821 cagcgcatgg gtcaaaaaca gcaggatcgc aagctgcccg accgacaccg ccgtcgaaaa
    17881 cagcgccagc cgcaaaaatg cacgcttttg cacgctgact acaggcgctg ctcggtcttg
    17941 ttccacaggc gggcgatgtt tccgcgatgg cggatcagga tcaagagcag aaacaccccc
    18001 gacagcacat agatgggctg gctggcatgg cacagataca gccacagcgg aaacagcagc
    18061 accgcgccga gcgaccccac agatgaaatt cgcgaaagac caaacagcac cgcccatgac
    18121 acaagcccaa gcagggccgg ccacggcgca agcgccacaa aacaacccaa ggccgtcgcg
    18181 acgcctttgc cacccgcgaa accggcaaac accggcagca agtgaccgac aaacgccgaa
    18241 agcgccacgg ctacggcaat ccactccttg tgcgggtggt ccgcgaaata tcgcagcgcc
    18301 aaaagcaccg gaatcgtcgc tttgagcgcg tccagcacaa gcaccagcgc gccaaagcct
    18361 ttgccgagcg ctcgcgccgc gttggttgcc ccgatgttgc ccgatccgac cgcccgcaga
    18421 tcgacgcggc ggagcttcgc aaagaccaag ccgtatggca ccgaacctga caggtacgaa
    18481 agcaaaaccg cgagcaaaat caccatcccc tgcacactac acccaagctc tacgagatca
    18541 gcgacttctc acagcagtgg ttccatagtc ccttgcgagc actccgtctt ctcgcaccat
    18601 actttccata gtcccctgcg agcactccgt cttctcgcac catactttcc atagtccctt
    18661 gcgagcactc cgtcttctcg caccatactt tccatagtcc cctgcgagca ctccgtcttc
    18721 tcgcaccata ctttccatag tcccctgcga gcactccgtc ttctcgcacc atactttcca
    18781 tagtcccttg cgagcactcc gtcttctcgc accatacttt ccatagtccc ctgcgtggcc
    18841 agagacctct cgctgggtcg cactatgggt ttggttggct gccgccgcaa aagcgtttca
    18901 gcgcgcgtgc ggactacttg tcgacagccg cgctgatcga gggacgggac aagacgtagc
    18961 gaatgataag gctggccagc ttgggcagtg gcgcgacttc gtggaccgcg ccggtctgga
    19021 tcaccgcttc gggcatgctg gcgatgaccg cggactgcgg tgactcgacg agcacacgac
    19081 ctgcggtgcg gcttacggcc tggacgccct gcacagcatc gccggacatg cccgacaaaa
    19141 tgacggccag caccgctgga cccatggctt cagcagcggc gctgagcatg cggtcgatgg
    19201 acgggacata gccgccgcgg ggctcttgta tctcgctggc tttgcgaacc cgcagcacgc
    19261 cgccgtctcg atacacgcag agcgagtggc tggccggggc gaccaagact tcccctgggg
    19321 ccaccggcaa gtccggatca gcctcgcgca cggaccactc ggtggtacgg cgcagcctat
    19381 cgacaaacgc cgccgtgaac ttgctcggca tgtgctgggt gataagcacc gaaatcggca
    19441 gcttgctcgg cagcgcttgc aaaagggtcg ctatcgccgg cggccccccc gtggacgcgc
    19501 cgatgcagac cagaaagcgc ggcagaagcg caggctccgg caagctgcgc atccccgaca
    19561 gcaggtcttc tgacgatcgg gtgcgcagcc tgacgccttg cccgtgaagc cgcatccgtt
    19621 cggcgaccga tcgaatgcta agttgcgtca ccgtccgcag cttgcgcagc agatcgtcgc
    19681 gaatcggctg caggtccgca tccgaggcga tgcgcgatgg cttggcgaca aactcgaccg
    19741 caccgagctc cagcgcgcga aagacgctct cgcgggtggc gtggcttgac acaatcacca
    19801 ccggacgtgg ctgcttggcc atcaacagcc gcaaaaaagc aaagccgtcg agcctgggca
    19861 tctctaggtc gagcgtgatc acatcgggtt gggtggcgaa aaccaccttc agaccttctt
    19921 cgccatcgct cgcctgaccg acgatttgca agtcgggttc gccgcgaaga agcgccgtca
    19981 gcgcctgccg aatcaccggc tggtcatcaa tgatgaccac tttcagcacc cggccactca
    20041 tgcaggcttc cgatagacca gctcgcgacc caagtgggca atcccgagcg gcgtattggc
    20101 gtcgagcagc gactccgcgt gacccagaaa cagataaccg ccaggtgcca gccggtccgc
    20161 aagacgctgt atgagcgatc cacggcttgc gcggtcaaag tagatgatga cgttgcgaca
    20221 gaagatggcg tcgaacggac cgccaatgct gctgaagtcg gcatccatca ggttgagctt
    20281 gtggaacgtg cagagcttgc gcaccgactc gatgacttgc cagcgcccgg tgagcggcct
    20341 gaaataccga ttgtgacgag cagcatcggt ctggcggaac gacgacggcc cgtacacggc
    20401 atgcatcgcg gtctgaagcg cctgcgaaga caagtcgctg ccaacgacat ggacctgcca
    20461 gccctcgaac agtccgcttt cgatgatctc gaccgccaaa gaataggttt cttcgccgct
    20521 ggcacagccg gctgaccaga tgttgagact gttgccgcgt ggccgcttgg catgaagctg
    20581 cggcaaaagc acctcgcgga acgcatcgag ctgatacgac tcgcggaaaa agtacgtctc
    20641 gcgcgtggac agaagctcaa atacttcggc aagctcacgg tcgcgcatga catggggaat
    20701 gcgcgggctc gtaagctgcg cgtagtactc ctgaaacgac gacagcccat gcgcctgcac
    20761 gcgcgacgca agccggcgtt ccagcagata ggccgtctcc ggggtgtaga ccaggccata
    20821 gcgggcttcg atcagcttgc gcaacagctc gaacgtcggg gtatcgagcg acaatgcgcc
    20881 gggttgcggc gaaggcatgg ccacttagtc ctgggacgtg ctcggataga acgtaagtgc
    20941 ttgctgcgcc gcctgcgccg tggcggggtt tttgtcttgg gtgccgtact tcagaagcga
    21001 gcgaatccgg ctgcgcagat tcgcgtcgcg gatgtcggca gaatgacgct ggaaaaactg
    21061 aaacgcgctg gcccggctgc gtggatcaag cgtcggtagc atgtcgcaca gcaccgagag
    21121 cgcatcgtga cccatctgaa gaatcgcttg gccggtcgtt tcggacaccg tcttgtcagc
    21181 gtcgcccagc gccagcacca gcggacgcag catttcccgt cgacccgtcc agccgagcag
    21241 cgccgcagtg gcctgccgaa ccgacggcgt tgcttcgaga agcgctcgca gcgatgaacg
    21301 aaccgccatt tcgtcgagcg ctttggccga atcaatcacc agcttgcggc ccgcgtcgtc
    21361 cagctgctga tacagtcgat acagtgccac cagcgccgct tcacgggcac cgcgcgcgcg
    21421 atcgagcagt ccgtcgatca gcaccggcac tgccgcagag tccttgctgt agcctagcga
    21481 cgtcatcatc gccggacgca gcgccaccgc ttctttgagc ggaaggactt cggcaatcgg
    21541 caagaccacc tgctgccggt ttagaccgtc gagcgccgcc agcacgacca gcatgtcggg
    21601 actgcgcagc gccgcacgca gcgccggcac gacctcttcg cttgaaaacg acgcaagctg
    21661 ttccatggcc gcggcgcgga cgttggggtc ttcgtcgagc agtgattgac cgagcgccgg
    21721 aatcacccgc gtgtcgccgt aggtccccag cacatcgacc agcaccttgc ggtagtccgg
    21781 cttttgcgcg agcgcggcaa gcagtggctc gatgcagacc ggccccagct tcatcacgcc
    21841 ttcgatgacg gcattgcgac ggccaatgtt gtccggctcg ctcatcgcat cgacaagctt
    21901 ctgggccaaa agcaccgtat ccgtaaagct cagcgccgaa gccaccgctt ccttgcggac
    21961 ctgccagctg tcgtcaccca tcacttcaat gagcaggtcg agcagcgcgc ggcgctccgt
    22021 ctcgtcttcc ggttgccggt taagcttctg gacggcggac aggcgagctt cttcctctcc
    22081 ctcacggaag atcgctgcaa gctgtgccag gtcataagcg gaattgtgca gggtcatgct
    22141 gattacgctc ccttggcggc ctggttgagc cccgccaagc tgattcgttc tgtcgtactc
    22201 aaaatcgcat caaggtcgag caccatcagg atgcgacctc ggtgatgaca aaccccagcg
    22261 aagtagcggg cattttcgtt ctgcgtcaaa agaggtgggg gccgaatcgc atcgcgtgac
    22321 acacgcagca cgtcggacac ccggtcgacg atcattccga ccagtcgacc ctctatcggc
    22381 acaatcaggt atttgcgagc ccgccggact gcatagtctt cgagctgctc ggcggtgagc
    22441 ggcggcaggt cgaagcgctt gcgcatatca accactggca aaatcgtacc gcgaagctcg
    22501 acgacgcctt cgatgaacga agccgcgcgc ggcacccggg taatcgccag cgggttgatg
    22561 atttctttga tccgcataat gtcgagcgcg tactcctcgt cgccgatgcg gaaaccgcac
    22621 agctgcacga tttcatcgcg cgtcttgtca cgctcgacag tcttgttgcc aaggccaaag
    22681 ccccctcgta tggatcgtcc ccggctcaaa acatcctccc ggaacgcatg acttcaaacg
    22741 acagcacttt gaccaagtcg agcaagatga tgatgcgctg cggctcggtg gtgatcatgc
    22801 ccggcccaaa gcgcgcttct cgtcgtgccg actcgtccac ttcggcgcgg atgcgtccga
    22861 tgccttcgac aaagtcggac tcttttcctg acagcacggt cggcggctca atgtcgactt
    22921 cgtgaatgcg taccacttcg tttacgcgat cgacgaccag cccaaagcgc tcgcgctctt
    22981 cttccgacac gttttcattg cttgcggcag gacgactgac aatcaagatg cgcgacttcg
    23041 ccgaaagcga agcagcccgc atccgcagtc gctgacgaag atccagcacg ggcatgacct
    23101 ggccacgaac cgcgatgatg ccggcgacaa agcccggaac ccgcggcact tcggtgacga
    23161 gtcgatgctt gatgatctcg cgaatgtgct caatgccgat gccgtactct tcttcggcga
    23221 tgtggaacgt cagcacgtcg cgagcaagac ggagctcgcc ggtcggatga cgctgcgatc
    23281 catcgtagtt gctgggcgat ccgctcggct gcgccatcac gccacctccg aaatacgcgg
    23341 catcgcttct tcggcaaccg cccgcacatc aagcaccagg gcggtctgct ggccacccag
    23401 ctccgtcgca cccgcaatgc caggaatgcc ttgcagcgtc ttgccgagcg acttgatgac
    23461 gatgtcctgc tgaccgacca agtcatcgac cagcaggccg agccggttct gcgccagcgc
    23521 caccacgacg acgtagccac tctgcggctc cggcatctcg ggcggacgct gaagcgaaaa
    23581 gatgtcttcg agccgaacca gaggcagcgt ctggtcgcgc agcgtaatca cctcgcggcg
    23641 ctcgatggtg cggatctgct tgtagtcgag catcaagctt tccatcaccg agt
//
LOCUS       DAORGN010000059        23688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_841_length_23688_cov_5.691364, whole genome shotgun sequence.
ACCESSION   DAORGN010000059 DAORGN010000000
VERSION     DAORGN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23688
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_23688_cov_5.691364"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /gene="msrA"
                     /locus_tag="PKL17_08755"
     CDS             complement(<1..559)
                     /gene="msrA"
                     /locus_tag="PKL17_08755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNK44857.1"
                     /translation="MFFLDPRKLKLPTAAEALPGRSEAMPVSGKHFVSGHPTVMPFPA
                     GIERAMFGMGCFWGAEKQFWLTPGVYSTAVGYAAGLTPNPSYYEVCSGRTGHNEVVLV
                     AYDPQQIRYEALLRAFWEGHDPTQGMRQGPDIGTQYRSGIYVYSPEQKKRAEASLAEY
                     QRALSAAGYGPITTEILPASAFYYAE"
     gene            639..2237
                     /locus_tag="PKL17_08760"
     CDS             639..2237
                     /locus_tag="PKL17_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44858.1"
                     /translation="MPAVEKKPKDAHVDPVKPTQTAPAAVQKPAVNVPWEALAPVPLE
                     HVPMPLGPVPTPDKLPPEVFSDSLGHYADAGFQPVKPAEPVSREKKIESYEQTLDGAN
                     YVQQHLQGQQKVDYLEDRLGALAQFSQGVAPATATGKQTETVPTQLIDDARPLIESLE
                     RMTPDATVKKAGTNAEGAHVGSGADWNTKLGVPQYRTQSDNLIPPEASCNMTSLAMAL
                     ERMGYGRENAMDAIDGELRQKYLDEQKAKLAKTKGDPSTLPKTADDVTLPAGYFEGQV
                     KGYLKAVNDSAEKPYQGVRGKTTNDKAWDAIAGQYRENAQFEDTLDFLRYLSKAGGRT
                     DLDTVTPKLLEKLEPDPDKRPTYRSVTPDRNMDWTKARGEMSNVLDEGGAAMLSYHHK
                     GGGNRDASHIISVQQMQKDGLIVDDPYGHIRPGYNVSQVGDAYGEKGQAGRSPDRKNQ
                     VDPAVNRALPSQMDSDWTASAGQHLKANESLGNSSSVADSVFANSWRSIRFLEPAKPK
                     PKAPVVEPAKVPAVPQQNPADPHK"
     gene            2343..3077
                     /locus_tag="PKL17_08765"
     CDS             2343..3077
                     /locus_tag="PKL17_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNK44859.1"
                     /translation="MTTGQHSDEKSYKTWAHAVEQQLAEHGSEERRKVAVTYFPTAMR
                     VLGVPVPALRTVAKGLAKQLKTEPPGVVVAHAQALVDAGTMEGRQVAYEILEAHKAAA
                     LSLGTVEIERLRVGLDNWASVDSFAASVAGPAWRRGQLPDRLVDAWAVSPDRFVRRLA
                     LVCTVALNMRSRGGKGDVARTLHVCRQLVGDRDEFVVKALSWALRELLPHDAAQVQSF
                     VAAHEAKLNRRIVREISNKLTKGTKN"
     gene            complement(3099..5147)
                     /locus_tag="PKL17_08770"
     CDS             complement(3099..5147)
                     /locus_tag="PKL17_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNK44860.1"
                     /translation="MPTDSDSPPSGAPTFADLGLSAALVRAVHDEGYTTPTPIQARAI
                     PVLLSGRDLLGQAQTGTGKTAAFALPLLQAIDPSVAAVQALVLCPTRELALQVATAVH
                     DYGKHMQTLSVLPIYGGDSMTRQLRRLSHGVQVVVGTPGRVLDHLERGSLSLDAVRFV
                     VLDEADEMLRMGFIEDVETILGKTPKDRQTALLSATLPPQIRRIAHNQLQNPEHVAIA
                     AATRTVDTIQQRFVLVEERHKTDALLRILEVEAPEAALIFTRTRIGADELAEVLTSEG
                     IPAEALHSDLSQAQRTTVLEKMRAQKLRVVVATDVAARGLDIDHISHVINFDLPMDLE
                     TYVHRIGRTGRAGRRGEALLFVTPRERGRLRLLERFTQSPLTPLPIPTRQMVIAKREE
                     RIAEELLPQLQHPVPDRFRALCQKLVEQSGHDAEAIAALLLSQKCADKNPSHRRVPDD
                     LQVALRPDRPLRDDRPAGRFSRSDDRPRFATRPDRPPRDEQPPRFFDRPARPPRDEQP
                     PRFFDRPPRDEQPTRSFDRPARPPRDEQPPRFFDRPARPPRDEQPPRFSDRPRHEPSD
                     EQAEPLFDRAPRSRSARSPRPDEVTKLLIAVGYEHGLRPQDVVGAIANEAGIPGRAIG
                     AIEIFPTSTVVELPMQLGTRVLTSLRRTYMRGQAAKPRMLSQLDEAPPSRKRPSRTKH
                     "
     gene            complement(5298..7265)
                     /locus_tag="PKL17_08775"
     CDS             complement(5298..7265)
                     /locus_tag="PKL17_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK44861.1"
                     /translation="MLSSVSFAVDSTDRIALVGVNGSGKSTLLRLLVGSHADPAFGAD
                     AAVHDPRESEPDAGLITRRRGLHVEYVPQEPKLPVTQTVQEALRQGMRRHAEVLAELE
                     TLAQDIETSDGEKRDAAIARQAELHEHIKDLGGFDVDHEIRGIAASLELPPMDAVIGN
                     LSIGERRRVALAAALLARPELLMLDEPTNHLDARTVEWLENRLRSQPGALVLVTHDRY
                     FLDRVATRILEIDRGRIYAYEGNYQRFLEKQAERLDTEASRERERAMFVRRELDWIRR
                     GPAARTTKQKARIDRFNEAVAQKPKAEDKSTVDRRLILRLPTGGRLGKTILEMRGISK
                     SIGGKKLFSDLQLILKPGDRIGIIGGNGAGKTTLVRTLIGELLPDAGEVVRGVNTRIA
                     FLDQGRAELDDSRSVLEEVAGDSDSVFLEDGPVHVRTFLRMLLFDDRFADAKVATLSG
                     GERNRVQLAKLLRRGGNLLILDEPTNDLDLLTLGVLEEALAEFPGCALVVSHDRWFLD
                     HVATGILAFEGNGKVSFHEGNYSDYLERTKGEAKADAESIGKTAKPQAPASKASAAAD
                     KTEPPKNQSKPRKLTFAEQNELAKMEENILAAESEAEALSQKLSDPSVYKDHATDVPG
                     WVASLDKKRADIERLYARWQELESLAAQSKK"
     gene            complement(7350..8411)
                     /locus_tag="PKL17_08780"
     CDS             complement(7350..8411)
                     /locus_tag="PKL17_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK44862.1"
                     /translation="MEPSSLGPVLVTGGTGFLGEHLLRALRGKGKAVRVLTREPTPEL
                     RELSVSVYQGDLLSDANRAEKASAKDVDLPENEALDAALDGVKEVYHLAGMVSRDPAR
                     TQTMMHLHVDGTRRLLHACKRAGVRRVLLASTSGTIAISREADPIPDESWPYPVDLCG
                     EWPYYLSKIYQEKLALELAPKLGLELVVVNPSLLLGPGDRRGSSTMDVRRFLCNEIPA
                     VPAGGINFVDVRDVAPACVAAMERGRNGQRYLLGGMNWTFAEFLGRLGRIARMDGPWL
                     KLPSRWNRLFEGAVKAVDELYRHRGQTSPIERISFEMSQHYWYCDSSLAMRELGFATR
                     DPAETLDDTIRDVRKSLRY"
     gene            complement(8465..9610)
                     /locus_tag="PKL17_08785"
     CDS             complement(8465..9610)
                     /locus_tag="PKL17_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK44863.1"
                     /translation="MAAIFFAFLDGAFRYDCARCGQACCRGKGVALAANRELVPLLRR
                     VPQVAPLLTPLPGGYVKLPDVTDGCWFLGDDGMCSYERSHGRDAKFTTCRLFPFNRVF
                     RVGSAQVVDVNSVVCPIEDALTTGHGVTHAELSAELSALGSSPLTESSVNLPPGAREL
                     RWLALETTLRDDSAQFLGEPDYLAFARHMQATTAKHLGLRGEIPDTIGRLGTYFTEIY
                     GALASPHGPAIARGLTLLSPSLRFNALFRKNADDYRRLIKAQPGQLLATFVQSLWAAA
                     TWNRPLSLRAQTELHQAQTDLRTLLARLHEPAQLHAALAVPDLPPELQDAAKRLHDRL
                     SRPTAAKQPVGDILVDIARELPSEKRALVPTLLLRAGESLRFVEAHP"
     gene            9700..10047
                     /locus_tag="PKL17_08790"
     CDS             9700..10047
                     /locus_tag="PKL17_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNK44864.1"
                     /translation="MISFVVSFVLYALAFMMTARLVPGIEVRSFRSAFGFAIVFAILD
                     GLLFKLLAFFTLPLVILSLGLFCLVIRAFLFLLTDKFIDGVKIDGFVPALIGSVLTGI
                     LNSGITWFLHKIF"
     gene            complement(10094..11776)
                     /locus_tag="PKL17_08795"
     CDS             complement(10094..11776)
                     /locus_tag="PKL17_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44865.1"
                     /translation="MQLVRIVPSLFLSAMILGGCGGGKKPPQLQVSKPAPIQVPVPAT
                     PASSDLWGYAKLRDPLGLAQKIFGPMTQPMLLSLGLGPNDLKPGSSAALYVWDPEGQP
                     LMAAPSALMLPFPAESERIAELSRAFAGTQALPIPSGTLFLLNPAAQAKAKTQKDAIL
                     QLGAAQSPFDVTVYAYLDPILKKHGPALRETLNKVGESVAVNGQNAALPGTGDSIKKL
                     YSNLVDSLIELKSATLAANLTDRALELSLISENKAPASKTEPYAVPDLLSFLPPSHLR
                     LQWNTRDMQRFVDFYLKTYAPMFESQPPIKEAMQKSLSEWSKAGQKISMAMSVTVGGD
                     NYIQMSSIMKVEDGKAALSAMRTGFQILMSPKVKESWDKLGISMTSKVSQGARKLHGW
                     PVDSYEYSYQVTKPEMQTAKQMFDKFSNLKMEVVQVGNYLVFAMGSSVDQAVNALMTG
                     KPEFPVEAMKRFPAGAAVYADIDLKRIAANVEKMMGRPLAIPLPQGAEVVSAASFEGS
                     AASQHQLFVPKALVDLGIGALMSAVQTRSRSNDDFGYDPAGPMGPPPGAPPP"
     gene            complement(11873..13231)
                     /locus_tag="PKL17_08800"
     CDS             complement(11873..13231)
                     /locus_tag="PKL17_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK44866.1"
                     /translation="MSILRRLRNLPQSAVHIAQQTFFSQRFRHYQHLRRQRFGMDLSA
                     LRTPGRPGLVSIVLPVFNGADLMSSAILSICAQTYSDWELVIINDGSNDNTAELAEHF
                     AARDPRIRVFHQENRKIPRTLSRGFRLADGEFFTWTSADNRLKPDFLSRFVDCFHRHP
                     DWDVAFANQDLIGEDGLPLRNSDYYCEYQTPKGSEHIYLPQRTDMLHSAGNLVGGAFM
                     YRSRAAHILGDYSPFRFTVEDYDYWLLADTLLGVHHVDFLSPVYEYRFHSGSLTSRAK
                     ELRIREATEKLLAWDRFRQDYCLSPLYVVLDASAQAADSPVWLDVKQQLRNASHRVIS
                     LSALLQHGFPTTHATAVYLRADLAAACRLPPPSGIPKGVLSAFLLCDQESTIPTDIHP
                     DWDVVLALGPGDVPHLGTQDTRYQGLWRSPDSHTLVRALDCRARVEALRTIETQTASA
                     KS"
     gene            complement(13228..14910)
                     /locus_tag="PKL17_08805"
     CDS             complement(13228..14910)
                     /locus_tag="PKL17_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44867.1"
                     /translation="MTSPLPRSGPRIPSAAGMSERWGLLLLTLVASLSLWGQLQLFLR
                     LRPGIPTAFLAVSTAFLLAKRISPRPLRDILSVCAIVALSAFAMGRIFDLSWDGQTYH
                     QEAIFQLAHGWNPIWDSPLPVWHGDITWCNSYPKGIWLAQAVFFRVFGNLESAKAVAV
                     SLLLSSLFLGYAAARSLDLKPGTSLLIAAAAALNPVVFCQLPSFYVDGALGSLLLILL
                     ALAVLFFQAPQRRHLVTMLLALSILCVTKFTGVVYAVFFSVGVGLSALLWKRKLVKPL
                     LVTTVLGGVLGFLFLGFQPYVTNLRSHLHPFHPLAGSQRIDFMPAQLGPEFAAQPRIL
                     TLAESLFSTSTNDKAWPRWKIPFAISRTELSEFGIPDIRIGGFGPWFGPVLGLSLLGV
                     LFLFVYHSRQKSLPRSRRELGTSHGKFLWPGLLVLAVILASSLVNPGVFWARYVPQLW
                     LIPVVVVVLLRLQGNRFAWALLLLLSMNAALVIGGSTNGQLARQHAIRNQLDEIRRQP
                     ITVNLQTFGAVRFRLQREHVDFRLGTLVCPAPKTMVSSLVEYCLRTPEGTQP"
     gene            complement(14939..16123)
                     /gene="wecB"
                     /locus_tag="PKL17_08810"
     CDS             complement(14939..16123)
                     /gene="wecB"
                     /locus_tag="PKL17_08810"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNK44868.1"
                     /translation="MHVLLAIGTRPEAIKLAPLYHALRRDPFFRVTTCITSQHRELLQ
                     QMLSFFDVRPDVDLQVMQPRQTLADLTCNVLRGADELLAEKRPDLVVVQGDTTTAFTV
                     GLAALYQRIRVAHVEAGLRSFDLKQPFPEEANRRLVDVFSDYLFAPTDGAADNLRKEG
                     YSQSSIFVTGNTGIDALLLAADIVRTQGHALPMNLPAGSKLALLTAHRRESFGDGLVR
                     ICQAVLQLLSQHPNLHILYPVHPNPNVQEAAQKYLAGHPRIFLCAPLEYPGFVAALAA
                     ADLILTDSGGVQEEAPALGKRVLVLRETTERPEGIDAGVAELVGTDVDKIVTRATALL
                     QGNAPKDRVLSPYGDGRASERIVEVLKTGALHSAFHLDTQDTAQDRPLQRVSQTRQLS
                     RS"
     gene            complement(16139..20590)
                     /locus_tag="PKL17_08815"
     CDS             complement(16139..20590)
                     /locus_tag="PKL17_08815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK44869.1"
                     /translation="MITLGCIYCASSRLTPAYQRADGNEIVACQDCGLLFIRDIPDDL
                     SALYGAEYFHKHDTATGQGYSDYASIAPISFRWQLALLRLWAGKNPLERRKLLDLGCA
                     NGLFLRLAASAGFGGTGVEISDAAAASVRDQGFPVIASAFETTEVPPCDVITAWDFIE
                     HVRDLHTVFEKVRLTLRPGGVFLFATPDGGSARAKTHGASWICLNSSFEHITYLTRPF
                     LQNALREVFGCDPILLSFDVGDHWTSIVGMVRVGGLTDEDQRISQLLSGQIPTAEEVR
                     RHGAELGYFLASFLQLPKLSDLLDQADGILPESTNAALRGSLLYLSDYHAEALDLLRK
                     GLADEPYLAAWLAAAEERVFAVREQELSAQLVQQKRSMEDRLARVRGENNHLREVNEQ
                     MLQSVTWKLGRKITEPIENIPHSRAVLDGLRQLRERGPMRSLSLARDYVEGVAGSAGI
                     PQRLRDRFTRKKNAPEETPSADSAQTPQPIPLASTGFPTQPAVDAVQTKQTQAVLPEP
                     DAILSTDPGRLRSQCLPLVSVILPVYNQTDLLRDSVLSVLSQSYPRIELVILDDGSRE
                     DVPAVLSGLLDLPSVRLFRQANQKLPRALSHAFQYARGELLTWTSADNLMHPHAVARL
                     CEALLRNPDAVLAYADVALIDDSGKPLTDGSYRPMYVDPQRPYVTRLTRSDVPLAAEN
                     DNYINACFLYRRSASEALGHIYADRLRGAEDYDFWLRLRTAGRLIHIGNEDPLYFYRV
                     HQRSMSHELLTQELAAHHQRICQLTELDSQRQRYAQKRWDVVIDSGLSPKTRSELQDA
                     LGQLPVQVLLQAGGAAKNSDRKTLSLLPPSQEPAHTSDFQQFVHVHPGHFALICRTPE
                     SKDSPRSERRIELPRGHNVHPLAIKARDVQKPGPVLPKAASGRPVFAIHIPFQRLQFT
                     VPQLHKLVSGNPWAYFLLINASSERCEELEHLTAELKNVGIVDERPLGEPYTLYAAID
                     WLLVPPCEPALGIGEQLDLLALSYAIRKPLIFANGHAQLVAPYQFVASEGESSLSFAR
                     SLRPDMMQIEVLHSYLESWTQERCLSTLLRHANTFAQDFAVPFPDTGIVPPPDSAPDP
                     VLPLITSSEPLKAAVLVDTLDVGGLEEVVAFLVRNLHRHGVRPTVTCLSHGGHVADKL
                     QREGHQVLVANGDPQRLHMQLQQLSPQIVNSHYASLPALEAANKLGRPIVETIHNTYV
                     WLDAERWKIEAQRSRFFAKGLAVSALVKRYYHAHNPQLAEDKVVVVGNAIDPDRIIPV
                     EQKIARAALGIPMDVTLFLCLGRYCPQKNQLGMIQGFAELAKTVPDAVLLCMGNTPKE
                     EQAYFDKLEALQKSLPCRDRIRFDSYHPDVSTVLSAADVLLMDSFFEGWSLAATEALL
                     TGTPLIHSLCGSAEELCGANSERGFVIPNPGGDIMKLELGPLLEIANRPDQPNIAALH
                     SAMAKAVQKRDEWRRERAAISTYARRMFHPTAILTRYAQTFFSFTRN"
     gene            complement(20770..22941)
                     /locus_tag="PKL17_08820"
     CDS             complement(20770..22941)
                     /locus_tag="PKL17_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK44870.1"
                     /translation="MRFRFSQALWAALLLLLGLPGPARAQNVGFQINRYEPTTVGEWS
                     FMVDHPWYSSTRYFAAGITLNYAHNPLVFGYSTVDNSFRQTYSIINHQLIGNVDLAGS
                     FLDRVTIAASVPVTLLERGQQIGDLAPVSGMAVGDPRISVMARIFGQPDGSAFSLSAG
                     ASVWIPLRAIDSSLPMLTSDAGVRVLPKIVAGGLAKKIRWSATLGFLYRPEATLGNYM
                     IPDGSTTGSALQLGVSAAYADTLRRFSIGPELLVSTDILSGHAFARDYTSLEVLLGVH
                     YNIAGYVQVGAAGGVGVLREPGTPDARALLRVAYAPMPKPEPPRVDSDGDGIIDRKDA
                     CPNDPGPATKDPRTNGCPPPDRDRDGVPDETDQCPTVPKGDRPDPNKLGCPIPDRDGD
                     GVLDAQDQCPTEAQGSEPDPKRPGCPDKDSDADGVVDSKDQCPQKPKGDHPDPGKLGC
                     PDSDADGDGVYDSVDQCPKVSAGGHPDPAKPGCPLPDRDGDSIVDAEDACPTKPGAPD
                     PNPKKNGCPGLVEIRRGLIVINRAVFFANDEAEILKNSYPVLEAVKNALQLQTQITKL
                     QIEGHTDDRGDAAHNTDLSKRRAQSVMQWLVDHGIAAGRLTAEGFGPNRPIADNKTLY
                     GRAKNRRVEFRILEPSGMVQAVSEAKPAGSPAAAQPTASPAAAPSDEPPPEAMEDGKG
                     KKDKKGKKDRGKKAKLDAGTDGGDDAKADKKKRRKKKKKDE"
     gene            complement(22953..>23688)
                     /locus_tag="PKL17_08825"
     CDS             complement(22953..>23688)
                     /locus_tag="PKL17_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNK44871.1"
                     /translation="AAGPGGGGGGGYVTLSPSTTVTTQANGGSGGTTASTDMLAFPRN
                     GASDGSLGQIVQTQLGPYAGAPYCSIADLSLTMSATPSVAHEIDPFQLVVNVQNLGPG
                     LSGNVQVAIDLPTGIKVQNIDAQNWSCRQTTRQMICKLATLPITQAPQIALTLIPALG
                     ATSLAFSATVSAPTTDPNLDNNSASVTIDNPAPLVARPAGGGFGCTMTTGTAGTSSTA
                     LWAMAFLLCGLRIVRRQRNWNGRIFG"
BASE COUNT         4738 a         6881 c         7405 g         4664 t
ORIGIN      
        1 cctcggcgta ataaaaggca gaagctggca ggatttccgt cgtaatcggg ccgtagcctg
       61 ccgccgaaag tgcgcgctga tattcggcca gtgacgcttc agcccgtttt ttctgctcgg
      121 ggctgtacac gtaaattccc gaacgatact gggtgccgat gtctggtccc tgccgcatgc
      181 cttgggttgg gtcgtggcct tcccaaaacg ctcgcagcag cgcttcatag cggatctgct
      241 gtgggtcata cgcgaccagc accacttcat tgtgtccggt acgacccgaa cagacttcgt
      301 agtagctggg gtttggcgta agtcccgcag cgtagccgac cgccgtgctg tacaccccag
      361 gcgtcagcca gaactgcttt tccgcgcccc aaaaacagcc cattccgaac atcgctcgct
      421 cgattccggc ggggaacggc atcaccgtgg gatgaccgga tacaaagtgt ttcccggaga
      481 caggcatcgc ttcactgcgg ccaggaagtg cttcggctgc cgtcggtaac ttgagcttgc
      541 ggggatctag gaagaacatg caccttatcc tagtacggtc gcggggcact tgccgcaggt
      601 ggtctgccga taagtgaagt gcgaaggggg aatggtctat gcctgctgtt gagaaaaaac
      661 caaaggacgc gcacgttgat ccggtcaagc cgacgcagac tgcgccggcg gcagtccaaa
      721 aaccggcggt caatgtgccg tgggaagcgc tggctccggt gccgcttgag catgtgccga
      781 tgccgctggg cccggtgccg acgcccgaca agctgccgcc cgaggttttt tcggattcgc
      841 tgggtcacta tgcagacgcg ggattccagc cagtgaagcc cgctgagccg gtgagccgcg
      901 aaaagaaaat cgagtcgtat gagcagacgc tcgacggcgc aaactatgtg cagcagcacc
      961 tgcaagggca gcaaaaagtc gactatttgg aagatcggct gggcgcgctg gcgcagtttt
     1021 cgcaaggcgt ggcaccagca acagcgacgg ggaagcagac ggaaacggtg ccaacccagc
     1081 tgatcgacga tgcccggcct ctgattgagt cgctggaacg gatgaccccg gacgcgacgg
     1141 tgaaaaaggc aggcaccaat gccgaaggcg cgcatgttgg cagcggtgca gactggaaca
     1201 ccaagctggg cgtgccgcag taccgcacgc agtcggacaa cctgatcccg cccgaggcgt
     1261 cgtgcaacat gacgagcctg gcgatggcgc tggaacggat gggctacggt cgcgaaaatg
     1321 cgatggatgc catcgacggg gagctgcggc aaaagtatct cgatgagcag aaagccaagc
     1381 tggcgaagac caaaggcgac ccaagcacgc tgccaaagac ggctgatgat gtgacgctgc
     1441 ctgccgggta tttcgaaggg caggtgaagg gctatctcaa ggcggtgaac gactcggcag
     1501 aaaagccgta tcagggcgtg cgtggcaaga cgacgaacga caaggcgtgg gacgccattg
     1561 ccggtcaata ccgcgaaaat gcccagttcg aggacacgct cgatttcctg cggtacttga
     1621 gcaaagcggg cgggcggacg gaccttgata ctgtgacgcc gaagctcttg gaaaaactcg
     1681 agcccgaccc cgacaagcga ccgacgtatc ggtcggtgac gccggaccgc aacatggact
     1741 ggaccaaagc gcgcggcgag atgtcgaacg tgctcgacga aggtggcgcg gcgatgctga
     1801 gctaccatca caaaggcggc ggcaaccgcg acgcatcgca catcatttcg gtccagcaga
     1861 tgcaaaaaga cgggctgatt gtcgacgacc cttacgggca catccgtcct gggtacaacg
     1921 tcagccaagt cggggacgcc tacggggaaa aaggccaggc ggggcgatcc ccggatcgca
     1981 aaaaccaggt cgatccggcg gtcaaccgtg cccttccgtc ccagatggac agcgactgga
     2041 cggcgtcggc aggccagcac ctgaaagcaa acgaatccct cggcaactca tcgtcagtgg
     2101 ctgacagcgt gtttgccaat tcgtggcggt cgattcgctt tctggaaccg gccaagccga
     2161 agccgaaagc cccggtggtc gagccggcca aagttcccgc ggtgccgcag caaaatccag
     2221 ccgatccaca caagtagcgt tcgttatctc gacggaagaa ggcctgtttc cgtctggtgt
     2281 cacggcgttt gctgtgccat cgtgccgacg ttcactgcgc aaaagcaatg tcggagttgg
     2341 cgatgacgac gggacagcac agcgacgaaa agtcgtacaa gacgtgggcg catgcggtcg
     2401 agcaacagct cgccgagcat ggcagcgaag agcgacggaa ggtcgcggtg acgtattttc
     2461 caacggcgat gcgggtgctc ggggtgccgg tgccagcgct gcggacggtg gccaaggggc
     2521 tggccaagca gctcaagacg gaaccgccgg gtgttgtggt cgcgcatgcg caagcgctgg
     2581 tcgacgcggg gacgatggaa ggccggcagg ttgcgtatga aatcttggaa gcgcacaaag
     2641 cggcggcgct gtcgctggga accgtcgaaa tcgagcggct gcgggtgggg ctcgacaact
     2701 gggcgtcggt cgattcgttt gcggcgtcgg tcgcggggcc ggcatggcgg cgcggtcagc
     2761 tacccgaccg cctggttgat gcgtgggcgg tgtcgccaga tcggtttgtc aggcggcttg
     2821 cgctggtctg tacggtggcg ctgaacatgc gatcgcgcgg cggcaaaggc gatgttgcgc
     2881 ggacgctgca tgtctgtcgc caactggtcg gcgatcgcga tgagtttgtg gtcaaagcgc
     2941 tgtcctgggc gctgcgtgag ctgcttccgc acgacgccgc ccaagtgcag tcgtttgtgg
     3001 cagcgcacga ggcgaagcta aaccgtcgca tcgtgcgcga aatcagcaac aagctgacca
     3061 aaggcacaaa gaactagcga cggcagggga cagtgggtct agtgcttggt gcgcgacgga
     3121 cgtttgcgcg acggcggggc ttcatccagc tgtgacagca tgcgcggctt ggcggcttgg
     3181 ccacgcatgt acgtccgacg cagcgaggtc aagacccgcg tcccaagctg catcggcagc
     3241 tcgaccacag tggacgttgg gaaaatctcg atcgcgccaa tcgcacgacc tggaatcccg
     3301 gcttcattgg cgatggcacc gacgacatct tggggtcgca ggccgtgttc atagccaacc
     3361 gcgatgagca gcttggtgac ttcatcgggc cgtgggctgc gcgccgaacg cgaacgcggg
     3421 gcccgatcga aaagcggctc ggcctgttcg tcacttggtt cgtggcgcgg gcggtcagaa
     3481 aagcgcggcg gctgttcatc gcgcggcgga cgggccgggc ggtcgaaaaa gcgcggcggc
     3541 tgctcatcac gcggcggacg tgccgggcgg tcaaacgagc gagtcggctg ctcgtcgcgc
     3601 ggcggacggt caaagaaacg cggcggctgt tcatcgcgcg gcgggcgggc cgggcggtca
     3661 aaaaagcgcg gtggctgctc atcacgcggc ggacggtcgg gtcgggtcgc aaagcgtggg
     3721 cggtcatcgc tgcgggaaaa tcgacccgcc ggtcgatcgt cgcgcagcgg acggtcggga
     3781 cgcagcgcga cctgcaagtc atcaggaacg cggcggtgcg acgggttttt gtcagcacac
     3841 ttttgcgaca aaagcagggc ggcaatcgct tcggcatcgt ggccactttg ctcgaccagc
     3901 ttttgacaca gcgcgcggaa acgatcgggc acaggatgtt gaagctgcgg cagaagttct
     3961 tcggcgatcc gctcttcacg cttggcaatc accatctgcc gggtgggaat cggcagcggc
     4021 gtgagcggac tttgggtgaa gcgttccagc aaccggagcc ggccccgttc gcgcggagtg
     4081 acaaacagca gcgcttctcc ccgtcggcca gcccgtcctg tgcgcccgat gcgatggaca
     4141 taggtttcca gatccatcgg caaatcgaaa ttgatgacgt ggctgatgtg gtcgatgtcg
     4201 agccctcgcg cggcgacatc ggtggcgacc accacgcgca gtttctgcgc ccgcattttt
     4261 tccagcaccg tcgtgcgctg cgcctgtgac agatcactgt gcagggcttc ggcggggatt
     4321 ccttcgctgg tcaggacttc ggccagctca tcggcgccga tgcgggtgcg ggtaaaaatc
     4381 agtgcggctt cgggcgcttc gacttccaga atccgcagga gcgcatcggt tttgtggcgt
     4441 tcttcgacca gcacaaagcg ctgctggatg gtgtcgacgg tccgggttgc cgcggcaatg
     4501 gccacatgct cggggttttg cagctggttg tgggcaatgc gtcgaatttg cggcggcagc
     4561 gtcgctgaca aaagcgcagt ttgtctgtct ttgggcgtct tgccgaggat tgtttcgaca
     4621 tcttcgataa agcccatgcg cagcatttcg tcggcttcat cgagcacgac aaaacgcacc
     4681 gcatcaagcg acagcgagcc tcgctcgagg tgatcgagca cccgccccgg cgttccgacc
     4741 acgacttgca cgccatggct cagccggcga agctgccgcg tcatcgaatc gccaccataa
     4801 atgggcagca ccgacagcgt ttgcatgtgc ttgccgtagt cgtggaccgc ggtcgccact
     4861 tgcagcgcca gctcgcgggt cggacacagc accagcgcct ggactgccgc aacgcttgga
     4921 tcaatcgctt gcagaagcgg cagggcaaac gctgcggtct tgccggtgcc ggtctgggct
     4981 tgccccagca gatcgcggcc cgaaagcagc actggaatgg cgcgcgcttg aatcggcgtc
     5041 ggtgtggtgt atccctcgtc gtggactgca cggacaaggg ccgcagaaag tcccaaatct
     5101 gcgaacgtcg gcgcaccgct cggcggcgaa tcactatcgg ttggcaaggg aagggcagaa
     5161 gcagtcgaag gggtcgccga agcgacggcg gggtcggtca aacaaggctc actttgtgaa
     5221 ggggaaaccg ggaaagccgg ctgcagttgc gcagccaaag ggacgcgaac tctacacggg
     5281 attgccaggt tgtgtgctta ttttttgctc tgggcggcca gcgattccag ctcttgccag
     5341 cgggcgtaca gccgttcaat gtcggcgcgt tttttgtcga gacttgcaac ccagccgggc
     5401 acatcggtgg cgtggtcttt gtacacgctg gggtcgctca gtttttgtga cagggcttcg
     5461 gcttcggact cggcagccag gatgttttct tccatcttgg cgagctcgtt ttgttccgca
     5521 aacgtcagct tgcgcggctt gctttggttt ttgggcggtt cggttttgtc cgccgcggcg
     5581 cttgctttgc tggctggggc ttggggtttt gcggtcttgc cgatgctttc ggcgtcggct
     5641 tttgcctcac ccttggtccg ttccaggtag tcgctgtagt tgccttcgtg aaagctgact
     5701 ttgccgttgc cctcgaaagc gaggatgccg gtggcgacgt gatcgagaaa ccagcgatcg
     5761 tgcgaaacga caagcgcgca gccggggaat tccgccagcg cttcttcgag cacgccgagt
     5821 gtcagcaggt cgagatcgtt ggtcggttcg tcgaggatga gcaggttgcc gccacgacgc
     5881 aacagcttgg caagctggac gcggttgcgt tcaccacccg acagcgtcgc gactttggcg
     5941 tcggcgaaac gatcatcaaa cagcagcatg cgcagaaacg tccggacgtg aaccggcccg
     6001 tcttccagaa aaacgctgtc gctgtcaccg gccacttctt caagcacgct tcggctgtcg
     6061 tcgagctcgg cgcggccttg gtccaaaaac gcaatccggg tgttgacccc gcggaccact
     6121 tcaccagcgt cgggcagaag ctcgccaatc agggtgcgaa ccagggtggt tttgccggcc
     6181 ccgttgccgc cgatgatccc gatgcggtcg ccgggtttta ggatgagctg caggtcggaa
     6241 aacagctttt tgccgccgat ggacttgctg atcccgcgca tttccaagat ggtcttgccg
     6301 agcctgccgc cggtcgggag ccgcaggatg agccgcctat cgacggtgga tttgtcctcg
     6361 gctttcggct tttgtgcgac ggcttcgttg aaacggtcaa tgcgggcttt ttgcttggtg
     6421 gtgcgggctg ctgggccgcg gcgaatccag tccagctctc gcctgacaaa catcgcccgt
     6481 tcgcgctcgc ggctggcttc ggtatcgagc cgctcggcct gcttttcgag aaagcgctga
     6541 taattgcctt cgtaggcgta gatccgtccg cgatcgattt ccaaaatccg cgtcgcgacg
     6601 cgatccaaga agtagcggtc gtgcgtgacg agcaccagcg cgccgggttg gctgcgcagg
     6661 cggttttcca gccactcgac ggtgcgggcg tccaggtggt tggtcggctc gtcgagcatc
     6721 agcagttccg gtcgggctag cagcgccgcg gccagtgcca cccgtcgccg ttcgccgatc
     6781 gagaggttgc caatcaccgc atccatcggc ggcagctcta gcgacgccgc aattccgcga
     6841 atttcatgat cgacgtcgaa gccgcccagg tctttgatgt gttcgtgcaa ctcggcctgt
     6901 cgggcaatcg ccgcatcgcg cttttcaccg tcgctggttt cgatgtcctg cgccagcgtt
     6961 tccagttccg cgagcacttc ggcatgacgg cgcatccctt ggcgcagcgc ttcctgcacg
     7021 gtctgggtga ccggaagctt gggttcttgc gggacgtatt cgacgtgcag cccgcgcctt
     7081 cgggtaatca ggccggcgtc cggttcgctt tcgcgcggat catgcacggc agcatcggcg
     7141 ccgaaggcag ggtcagcatg tgaaccaacg agcagccgca gcagcgtcga tttgcccgag
     7201 ccgttcacac ccacaagtgc aatgcgatcc gtcgagtcga ccgcaaacga aacactggag
     7261 agcacacgac gcgcgccgaa gtttttttca accccttgaa cggtgaggat tgtcgtcaca
     7321 agccgccttt gccgagaaat gggggggcgt tagtagcgca ggctcttgcg gacgtcgcga
     7381 atcgtgtcgt cgagcgtctc ggcgggatcg cgggtggcaa aacccagctc acgcatggcc
     7441 aggctgctgt cgcagtacca gtagtgctgg gacatttcga agctgatgcg ttcaattggg
     7501 ctggtctggc cgcggtggcg atacagctcg tcgacggcct tgaccgcgcc ttcaaacagg
     7561 cgattccagc gcgacggcag cttgagccat ggcccatcca tgcgggcaat gcggcccagt
     7621 cgaccgagaa actcggcaaa cgtccagttc atgccaccga gcaagtagcg ctgtccgttt
     7681 ctgccccgct ccatcgccgc cacgcaggct ggggcaacat cgcgcacgtc gacaaagttt
     7741 atgccgccag cggggaccgc ggggatttcg ttgcacagaa agcgacggac atccatcgtc
     7801 gatgaaccgc gacggtcgcc cggcccgagc agcaggcttg ggtttacgac gacaagctct
     7861 agtcccagct tcggcgcaag ctctagcgcg agcttttcct gataaatctt ggacaagtag
     7921 taaggccact cgccgcagag atcgacggga tatggccagc tttcatcggg aatcggatcg
     7981 gcctcgcggc ttatcgcaat ggttcccgat gtactggcga gcagcacgcg gcggactccg
     8041 gcgcgcttgc aggcatgcaa aagccgccgg gttccatcga cgtgcagatg catcatcgtt
     8101 tgggtccgcg ccggatcgcg cgacaccatt cccgccaagt gatagacctc tttgacacca
     8161 tcgagggctg cgtcgagcgc ttcgttttcc ggcagatcga catcttttgc ggatgctttt
     8221 tcggcgcggt ttgcatcgga caaaaggtcg ccctgataga ccgataccga cagctcgcgt
     8281 agctccggcg tcggttcccg tgtgagcacc cgaaccgctt tgcctttgcc ccggagcgcg
     8341 cgcagcagat gctctccaag aaatcccgtc ccacctgtaa ccagaaccgg ccctaaagac
     8401 gagggctcca ccacttgacc gtttgcgttc acgaccgggg acgataaccc aggccgcgct
     8461 tcagtcaagg gtgtgcctcc acaaagcgca gcgactcgcc ggcacgcaga agcagggtcg
     8521 gcacaagggc ccgcttttcc gacggaagtt cccgcgcgat gtcgacaagg atgtccccaa
     8581 cgggctgttt ggcagcggtt gggcggctga gccggtcgtg aagtcgcttc gcggcgtctt
     8641 gcagctctgg cggcagatca ggcacggcca acgcggcgtg aagctgggcc ggttcgtgca
     8701 ggcgtgcaag cagcgtgcgc agatcggtct gggcttggtg cagttcggtc tgggcgcgca
     8761 gtgaaagtgg tcgattccac gtcgctgccg cccacaatga ctgcacaaaa gtcgcaagaa
     8821 gctgccccgg ctgcgctttg atgagccgac ggtaatcatc ggcgtttttg cgaaacaagg
     8881 cgttgaagcg caaagacggc gacagcaggg tcaggccgcg cgcgatggct ggtccgtgcg
     8941 gcgacgcaag cgcaccgtat atctctgtga agtaagtccc aagccgtcca atcgtgtcgg
     9001 ggatttcgcc gcgcaagccc agatgtttgg cggtggtcgc ttgcatgtga cgggcaaacg
     9061 ccaggtagtc cggctcaccc agaaactgtg cagagtcgtc gcgcagcgtg gtttcgagcg
     9121 ccaaccagcg cagctcgcgg gcgcccggcg gcaggttgac gctgctttcg gtgagcggac
     9181 tgctcccgag cgccgaaagc tcggcagaaa gttcggcgtg ggtgacgccg tggcctgttg
     9241 tcagcgcatc ttcgatggga caaacgacgc tgttgacgtc gacgacctgg gccgatccaa
     9301 cacgaaagac gcgattgaag gggaacagcc ggcaggtcgt gaacttggcg tcgcgaccgt
     9361 ggctgcgctc gtaactgcac atgccatcgt cgccgagaaa ccagcagcca tccgttacgt
     9421 cggggagctt gacgtacccg ccgggaagcg gcgtaagcag cggcgcaacc tggggcactc
     9481 gacgcaagag cggcacaagc tctcggttgg ctgcgagcgc gacgcctttg cctcggcagc
     9541 aggcttgacc gcagcgggcg cagtcatagc ggaaggcacc gtccaaaaag gcaaaaaaaa
     9601 tcgcggccat agcccatctt accatgcggt ggcgcgcgga ccggaaggca ggcaagatga
     9661 agtcgtggcg ccacttcgcg cccggaggcc aaaacgatca tgatctcgtt tgttgtttcg
     9721 tttgtcctgt atgccctggc gttcatgatg acggcccgcc tggtgccagg aatcgaggtc
     9781 cgctctttcc gcagcgcgtt tggttttgcc atcgtgtttg cgatcctgga tggcctgctg
     9841 ttcaagctgc tggcgttttt caccctgccg cttgtgatcc tgtcactcgg tctgttctgt
     9901 ctggtgattc gcgcgtttct gtttctgctc accgacaagt tcatcgacgg agtgaagatc
     9961 gatggctttg tcccagcgct cattggatcg gttctgaccg gcatcctgaa cagcggaatc
    10021 acctggtttc tgcacaagat tttctaggcg ggaaaagacg gcgagcggcg acgggttgca
    10081 tgtcgccgat gctttacgga ggcggggcgc ccggcggtgg gcccatcgga cctgccggat
    10141 cgtacccaaa gtcatcgttt gatcggctgc gcgtttgcac tgcactcatg agcgcaccga
    10201 ttcccaaatc aaccaaggct ttggggacaa agagttggtg ctggctggcg gcgcttcctt
    10261 caaacgacgc tgcggacacc acttcggcgc cttgcggaag cggaatcgcg agcggacgac
    10321 ccatcatttt ttcgacgttg gccgcaattc gcttgagatc gatatcggca tagaccgcag
    10381 cgccggctgg gaaacgcttc atcgcttcga ccggaaattc cggcttgccg gtcatcaggg
    10441 cgttcaccgc ttgatcgacg ctgctaccca tcgcaaagac caagtagttg ccgacttgca
    10501 ccacttccat tttcaggttc gaaaacttgt cgaacatctg cttggcggtc tgcatttcgg
    10561 gcttggtgac ttggtagctg tattcgtagc tgtcgacggg ccagccgtgc agtttgcgcg
    10621 ctccttggct gactttgctg gtcatcgaaa tgcccagctt gtcccacgac tccttgacct
    10681 tgggcgacat caggatttgg aagccggtgc gcatggccga aagggctgct ttgccgtctt
    10741 ctactttcat gatcgaagac atctgaatat agttgtcgcc accgacggtg acagacatcg
    10801 ccatgctgat cttttggccg gctttgctcc attccgaaag cgacttctgc atcgcttcct
    10861 tgatgggcgg ctgcgattca aacatcggcg cgtaggtctt caggtagaag tcgacaaagc
    10921 gctgcatgtc gcgcgtgttc cactgaagtc gcagatggga cggcggcaaa aacgacagca
    10981 gatccggaac ggcgtagggt tcggttttgc tcgcgggcgc tttgttttcg ctgatcagcg
    11041 atagctccag tgcgcggtcg gtcaaattgg cagccagcgt cgccgacttg agctcgatca
    11101 ggctgtcgac caagttgctg tacagctttt tgatgctgtc tccggtgccg gggagcgccg
    11161 cgttttgtcc attgacagcg acggactcac ccactttgtt gagcgtttca cgcagagccg
    11221 ggccgtgctt tttcaggatc gggtccaggt acgcatagac cgtcacgtca aacggacttt
    11281 gcgccgcccc aagctgcaag atggcgtctt tttgcgtctt tgctttggct tgcgcggcgg
    11341 ggtttagcaa aaacagcgta ccggacggaa tcggcagcgc ttgcgtgcca gcaaaggcgc
    11401 gcgacagctc cgcgatgcgc tcactttcgg cagggaaggg caacatgagt gccgacggcg
    11461 cggccatcag cggctggcct tctgggtccc acacatagag cgcggcactg ctgccaggtt
    11521 tcagatcatt tggacccagt ccaagcgaca gcagcatcgg ctgcgtcatc gggccaaaga
    11581 ttttctgcgc caaaccgagc ggatcgcgca gcttggcata tccccacaga tccgagctgg
    11641 ccggcgtggc gggaacggga acttgaatcg gcgcaggctt ggatacctga agctgcggcg
    11701 gtttttttcc gcctccacag ccacccagga tcatcgcgct cagaaacaac gacggaacga
    11761 tacgaacaag ctgcatggaa tgcctcaaat gtcggtttta caagaacaag cggcgccaag
    11821 ctaaaccgca tttgggtcct acactcaagc acttgcgtgc gcttttttgc ggctagctct
    11881 tcgctgatgc ggtctgtgtt tcgatggtgc gcagggcttc gacgcgggcg cgacaatcca
    11941 gggctcttac aagggtgtgg gaatcggggc ttcgccacag tccttgatag cgggtgtctt
    12001 gcgtcccaag gtgcggcacg tcccctggtc ccaaggccaa gacaacatcc caatccggat
    12061 gaatgtccgt cggaatcgtg gattcctgat cgcacaacag gaatgccgaa agcacgcctt
    12121 tgggaatgcc gcttgggggg ggaagtctgc atgcggctgc caagtctgcg cgcagataga
    12181 cagccgtcgc gtgcgtggtg ggaaagccgt gctgcaaaag agcagacaag gaaatgacgc
    12241 ggtgggaagc gttgcgaagc tgctgtttca cgtcgagcca gactggggaa tccgcagcct
    12301 gtgccgatgc atcgagcacg acatacagtg gcgaaagaca gtaatcctgg cgaaagcgat
    12361 cccacgcgag cagcttttcg gttgcttcgc gaatccgcag ctctttggcg cgcgatgtga
    12421 gcgatccgct gtgaaaacgg tattcataca caggcgacaa aaagtcgacg tgatgaaccc
    12481 caagcagggt gtctgcgagc agccaataat cgtaatcttc gacggtaaaa cggaacggac
    12541 tgtaatcgcc aaggatgtgc gcggcgcggc tacggtacat aaacgcgcct ccgaccaggt
    12601 tgccagcgga gtgcaacatg tccgttcgct gcggaagata aatgtgctcg ctgcctttgg
    12661 gggtctggta ttcgcagtag tagtcgctgt ttcgcagcgg cagtccgtct tcgccgatca
    12721 gatcctggtt tgcaaacgcc acatcccagt ccggatgacg gtggaaacag tcgacaaagc
    12781 gcgaaaggaa gtcgggtttt agtcggttgt cagcgctcgt ccaggtgaaa aactcaccgt
    12841 cagccaaacg gaatccgcgc gacagtgtgc gaggaatttt gcggttttcc tgatgaaaga
    12901 cgcggatgcg agggtcgcgc gcggcgaagt gttctgcaag ctctgcggtg ttgtcgtttg
    12961 agccgtcgtt gatgatgaca agttcccaat cggaataggt ctgcgcgcag atcgacaaaa
    13021 tggctgatga catcagatcg gcgccgttaa acaccggcag cacaatcgag acaagtccgg
    13081 gtcgccccgg tgtccgtagc gcagacagat ccattccgaa ccgctgtctg cgcaggtgtt
    13141 ggtagtggcg aaagcgctgc gagaaaaagg tctgctgcgc aatgtgaacg gcactctgcg
    13201 gcaggttgcg cagacggcga aggatgctca tggctgggtt ccttcgggcg tgcgaaggca
    13261 gtattccaca agcgaactga ccatcgtttt gggcgcagga cagaccagcg tacccaggcg
    13321 gaagtcgaca tgttcacgct gcaaacggaa tcgcaccgca ccaaacgtct gcaggttcac
    13381 cgtgatcggc tgccgtcgga tttcgtctag ctgattccga atcgcgtgct gccgggcaag
    13441 ctgtccgttt gtactgccac cgatcacgag tgcggcgttc atggacagca gcaaaagaag
    13501 cgcccatgca aaccgattcc cttgcaaacg gagcagtacc acaacgacca caggaatcag
    13561 ccaaagctgc ggtacgtagc gtgcccagaa gacccccgga ttgaccagac tcgatgccaa
    13621 gatcaccgcc aacacaagca gtccgggcca taggaatttg ccgtgcgatg tccccagttc
    13681 tcgccgcgaa cggggcaggg atttttggcg ggagtgataa acaaacagaa acaggactcc
    13741 cagcagcgaa agcccgagca ccggaccaaa ccagggacca aatccgccga tgcgaatgtc
    13801 gggaatccca aactcggaaa gctcggtgcg gctgatggca aatggaattt tccagcgcgg
    13861 ccaggctttg tcgttcgtcg atgtcgaaaa cagcgactct gcaagcgtca aaatccgagg
    13921 ctgggcggca aattccggac ccagttgcgc cggcataaaa tcaatgcgct gcgatccggc
    13981 gagtggatgg aacggatgca ggtggctgcg cagattggtg acgtagggct gaaagccaag
    14041 aaacaggaat cccagtacgc cgcccagcac cgtggtgaca agcaggggtt tgaccagttt
    14101 ccttttccac aaaagtgcag aaagcccgac gccgaccgaa aaaaatacgg cgtagacaac
    14161 cccggtgaac ttggtcacgc acagaatgga aagggccagc aacatcgtca ccaaatgtcg
    14221 tctctgcgga gcctgaaaaa acagcaccgc aagcgcgagc agaatcagca gaagagagcc
    14281 cagtgcacca tcgacataga agctgggaag ctgacaaaac accaccggat tgagcgccgc
    14341 cgccgctgcg atgagcagac tggttccggg tttcagatcc aagcttcgcg ccgctgcata
    14401 acccagaaac agcgatgaca aaagcagtga caccgcgact gctttggccg attccaggtt
    14461 gccaaatacg cggaagaaca ccgcctgcgc aagccagatt ccttttggat aggaattgca
    14521 ccaggtgatg tcgccatgcc acacaggaag cggtgaatcc caaatcggat tccagccgtg
    14581 ggcgagctga aaaatcgctt cctgatgata cgtctgtcca tcccagctca agtcgaaaat
    14641 ccgtcccatc gcaaatgcag acagcgccac gatggcacag acagacagga tgtcacgcag
    14701 tggtcgcggc gagattcgct tggccaaaag gaaggccgtg gacaccgcca aaaaagcagt
    14761 cggaatcccc ggacgcagac ggagaaagag ctgaagctgt ccccacagag acagcgaagc
    14821 gacaagcgtc aaaagcaaaa gtccccatcg ctccgacatc ccggcggcag atgggatgcg
    14881 cggtccagat cggggcagtg gcgaggtcat cgggcggttc cttgcattcc ctggcggctc
    14941 agctgcggct cagttggcgc gtttggctga cacgctgtag cggtctgtct tgggcggtgt
    15001 cttgcgtgtc cagatggaat gcactgtgca gcgcgccggt ctttaagact tcgacgatgc
    15061 gttctgatgc gcgaccatca ccatagggac ttagcacgcg gtctttcggc gcattccctt
    15121 gcagaagtgc cgtcgcccgc gtcacgattt tgtcgacatc ggtgccgacc agctcggcga
    15181 ctccggcatc aattccttcc ggtcgttcgg tggtttcacg cagcaccaga acccgtttgc
    15241 caagcgccgg cgcttcttcc tgcacgcctc cggaatcagt caggatcaga tccgcggcag
    15301 caagtgcagc gacgaaaccc ggatattcga gcggtgcgca caggaaaatg cgtggatgtc
    15361 ctgccaagta cttttgcgcg gcttcctgca cgttgggatt gggatgaacc ggatacaaaa
    15421 tgtgcaagtt cggatgctgg gaaagcagct gcaggaccgc ttgacaaatc cgaaccagtc
    15481 cgtcgccaaa cgactccctt cgatgcgccg taagcagggc cagcttcgat cctgcgggca
    15541 ggttcatcgg cagcgcgtgg ccttgcgtgc gcacgatgtc tgccgcgagc aacagcgcat
    15601 caatgcccgt gtttccggtc acaaagatcg agctttgcga atagccttct ttgcgcagat
    15661 tgtccgccgc accgtcggtg ggtgcgaaca gataatcgga aaatacatcg accaagcggc
    15721 ggttggcttc ttctggaaat ggctgtttca gatcaaagga acgcagcccc gcttccacat
    15781 gcgccacccg aatccgctga tacagcgcgg caagtcccac cgtaaatgcc gtcgtcgtgt
    15841 cgccttgcac gaccaccagg tcgggccgtt tttcggccag cagctcgtcg gccccacgca
    15901 gtacgttgca ggtcagatcc gcgagtgtct gccgcggctg catcacctgc aagtcgacat
    15961 cgggacggac atcgaaaaag gaaagcatct gctggagcag ctcgcggtgt tgcgacgtga
    16021 tgcaggtcgt cacgcggaag aacggatcac gccgcagcgc atgataaagc ggcgccagct
    16081 tgatcgcttc cggtcgtgtc ccaatcgcta aaaggacatg catggtgggt tcctggtctt
    16141 agtttctggt aaaggaaaag aaggtctggg cgtagcgcgt aaggatggcc gtcggatgaa
    16201 acatccgtcg agcataggtg ctgattgcgg cccgttcacg gcgccactca tcccgttttt
    16261 gtacggcttt cgccatcgcg gaatgaagcg ctgcgatgtt gggttgatcg ggtcggttgg
    16321 caatctccaa aaggggtccc agttccagct tcatgatgtc gccgcctgga ttgggaatca
    16381 caaagccgcg ctcggaattt gcaccgcaca gctcttctgc gctaccgcac aagctgtgga
    16441 tgagcggcgt tccggtcagc agcgcttcgg tggcggccag actccagcct tcgaaaaagg
    16501 aatccatcag aaggacatcg gctgccgaca acaccgtcga gacatcagga tgataggaat
    16561 cgaaccggat gcggtcgcga cagggcaagc tcttttgcag cgcttcgagt ttgtcaaagt
    16621 acgcttgctc ttctttgggc gtgtttccca tgcacagcag aaccgcgtcc ggaaccgtct
    16681 tggccagctc tgcaaacccc tgaatcatgc caagctgatt tttctgcgga cagtaccgtc
    16741 ccagacagag aaacagcgtg acatccatcg gaatcccaag cgcagcgcgc gcgatcttct
    16801 gttccaccgg aatgatccga tccggatcga ttgcattgcc gaccaccaca actttgtctt
    16861 ccgcaagctg cgggttgtgg gcgtggtagt agcgttttac aagcgcgctg acggccagac
    16921 ctttggcaaa aaagcggctc cgctgggctt cgattttcca gcgctctgca tcgagccaga
    16981 cataggtgtt gtgaatcgtt tcaacgatcg gacgacccag tttgtttgcc gcttcgagcg
    17041 ccggcagact cgcgtaatgg gaatttacga tctgcgggga aagctgctgt agctgcatgt
    17101 gcagtcgctg cggatctccg tttgcaacca ggacttgatg tccttcgcgc tggagtttgt
    17161 cggccacatg accaccatgg gaaaggcacg tcaccgtcgg acggaccccg tgacgatgca
    17221 ggttgcggac caaaaacgcg acgacttctt ccagaccacc gacatcgagc gtatcgacca
    17281 gcaccgcggc tttgagcggt tcggaagacg tgatgagcgg cagcactgga tcgggcgcag
    17341 aatcgggtgg cgggacaatt ccggtatctg gaaacggaac cgcaaagtcc tgcgcaaagg
    17401 tgtttgcatg acgaagcagc gtcgaaagac agcgttcctg ggtccacgat tccaaatagg
    17461 aatgaaggac ttctatctgc atcatgtcgg gacgcagact gcgcgcaaag gaaagagacg
    17521 actctccttc gctggccaca aactggtacg gcgcgacaag ctgggcgtgt ccgtttgcga
    17581 agatgagcgg cttgcgaatg gcgtaggaaa gcgcgagcag atcgagctgt tcaccgattc
    17641 ccagcgccgg ttcacaaggt ggcacaagca gccagtcaat cgccgcatac agcgtgtacg
    17701 gctccccgag cggcctctca tcgacgatgc cgacgttctt cagttccgcg gtcaggtgtt
    17761 ccagttcttc gcagcgctcg ctcgacgcat tgatcagcag aaagtacgcc cacgggttgc
    17821 cggacaccag cttgtgtagc tgcggaaccg tgaactggag tcgctgaaac ggaatgtgaa
    17881 tcgcaaagac cggacggcca gatgcggctt tcggtagcac gggaccgggc ttttgcacgt
    17941 cgcgcgcttt tatcgccagc ggatgtacgt tgtgtccgcg cggaagctcg atgcgacgct
    18001 cgctgcgggg actgtctttg ctttccgggg ttcgacagat cagcgcaaaa tggccgggat
    18061 gaacgtgcac aaactgctga aagtccgatg tatgcgccgg ttcttgtgaa ggcggaagaa
    18121 gcgacagtgt cttgcggtca ctgttttttg cagctccccc ggcttgcaaa aggacctgga
    18181 ccggaagctg accgagtgca tcttgcagct cgctgcgggt ctttggggaa agcccactgt
    18241 ctatcaccac gtcccagcgt ttttgtgcgt atctctgccg ctgggaatca agctctgtca
    18301 gctgacagat tcgctggtgg tgtgccgcca gttcttgcgt caacagctcg tgactcatcg
    18361 agcgctgatg gacgcgatag aaatacagcg gatcttcgtt gccgatgtga atcagtcgac
    18421 cggctgtgcg cagccgcagc cagaagtcat agtcttccgc gccgcgcaga cggtcggcat
    18481 agatgtgtcc cagtgcttcc gatgcgctgc ggcgatacag aaaacaggca ttgatgtagt
    18541 tgtcgttttc cgcggccagc ggcacatcgc tgcgggtcag tcgggtcaca taaggacgct
    18601 gcggatcgac atacatcgga cgataggaac cgtcggtgag cggtttccca ctgtcatcaa
    18661 tcagcgccac atcggcatac gccagcaccg cgtccgggtt gcgcaagagc gcttcacaga
    18721 gtcgtgccac cgcgtgtgga tgcatcaagt tgtccgcaga tgtccaggtt aggagttcgc
    18781 cgcgggcata ctgaaacgca tggctgagcg cgcgcggcaa cttctgattg gcctgacgga
    18841 agagacgcac cgacggaaga tccagcagtc ctgacagaac cgcaggaacg tcttcgcggg
    18901 atccatcatc cagaatcaca agctcgatgc gtggatagct ctgtgaaagg acgctaagta
    18961 cagagtcgcg caacagatcc gtttggttat agaccggcaa gatcacgctg acgagcggca
    19021 gacactggct gcgcagtcgc cctgggtccg tcgacaaaat ggcatcaggt tctggcagga
    19081 cggcctgggt ttgtttcgtc tgtaccgcat ccacagctgg ctgtgtaggg aacccagtgg
    19141 aggctagcgg aatcggctgc ggagtctggg cagaatccgc gctgggtgtt tcttccggcg
    19201 cgtttttttt ccgcgtgaat cgatcgcgga gtcgctgcgg aattccagca ctgcccgcga
    19261 ctccttccac atagtcccgc gcaagcgaca gagaacgcat cggaccccgc tctcgcagct
    19321 ggcgaagtcc gtcgagcact gcgcgactgt gggggatgtt ttcaatcggt tcggtgatct
    19381 tgcgacccag cttccacgtc accgattgca gcatctgttc attgacttcg cgcaggtgat
    19441 tgttttcacc acgcacccgc gcaagccggt cttccatgct gcgtttctgc tgcacaagct
    19501 gcgccgaaag ctcttgctcg cgcaccgcaa agacgcgctc ctctgccgcg gccagccatg
    19561 cggccagata gggttcgtcg gccagtcctt tgcgcagaag atccagcgct tcagcgtggt
    19621 agtccgacag atacaaaagc gatccgcgca gcgccgcatt tgtgctttct ggcaggattc
    19681 cgtccgcttg atcaagcaga tcggacagct tgggaagctg caaaaaggac gcgaggaaat
    19741 agccaagctc agcgccatgg cggcggactt cctctgcggt gggaatctgt ccagacagaa
    19801 gctgtgaaat gcgctgatct tcgtcggtaa gtccaccgac acgaaccatg ccaacgatgc
    19861 tggtccagtg gtccccgaca tcgaagctaa gcaggatcgg atcgcagccg aagacttccc
    19921 gcagcgcgtt ttgcaaaaag gggcgtgtca aatatgtaat atgttcaaac gagctgttca
    19981 gacagatcca gcttgcgccg tgtgtcttgg cgcgcgcaga accaccgtcg ggcgtggcaa
    20041 acaggaagac gccccctgga cgcagggtca aacgaacttt ttcaaacacg gtgtgcaagt
    20101 cacggacgtg ttcgatgaaa tcccatgcgg tgatcacatc gcagggaggg acttccgtgg
    20161 tttcaaatgc ggacgcaatg accgggaaac cctgatcgcg cacagatgca gcggcagcat
    20221 cgctgatctc gacgccagtg ccgccaaaac ctgcgctggc ggccaagcgc aaaaacagtc
    20281 catttgcaca gccgaggtcg agcagcttgc gtcgttcaag tgggtttttt ccggcccaca
    20341 aacgcagaag cgcaagctgc cagcggaagg aaatcggcgc aatcgacgca tagtcgctgt
    20401 agccttgtcc cgtcgctgtg tcgtgtttgt gaaaatactc tgcgccatac agcgcgctca
    20461 agtcatcggg aatgtcgcgg atgaacagaa gcccgcagtc ttggcacgcg acgatctcgt
    20521 ttccatcggc gcgctgataa gcgggcgtca gacgggatga cgcacaataa atgcaaccaa
    20581 gcgtaatcat ttggggcccg tataacgcaa gcccgcgacg gctgtcaacg actgcttggc
    20641 ggttcgcagc ggcgcagatc cgtcggggtc tgcctgcgca acctgcgcgt caacgattgg
    20701 aagtgagaac ggaaagggaa ggaaaacggt tgggctggct ttgggcgtcg acggatgacg
    20761 agctacggac tactcatcct ttttcttttt tttgcggcgc tttttcttgt ccgcttttgc
    20821 gtcgtcacca ccgtctgtac cggcatctag cttggctttt ttgccgcggt cttttttgcc
    20881 cttcttgtct ttctttcctt tgccgtcttc catcgcttcg ggcggcggct catcgctcgg
    20941 ggccgcagcg ggcgaagcgg tgggctgggc cgcggcagga ctgccggcag gcttggcttc
    21001 agacacggct tgcaccattc cggatggttc caaaatccgg aattccacac gtcgattttt
    21061 ggcacgaccg tacagcgtct tgttgtcggc aatcgggcgg tttggaccaa atccctctgc
    21121 ggtcagacgg cccgcggcaa ttccgtggtc aaccagccac tgcatgacgc tctgtgcccg
    21181 ccgcttcgac aggtcggtgt tgtgcgccgc atcgccacga tcgtcggtgt gaccttcgat
    21241 ctgaagtttg gtgatctgcg tctgaagctg gagcgcattt ttcaccgctt ccagcaccgg
    21301 atagctgttc ttgaggatct cggcctcatc gttggcaaag aacactgcgc ggttgatgac
    21361 aatcaatccg cgacggattt cgaccaggcc ggggcagcca ttctttttcg gattgggatc
    21421 gggcgcgccc ggtttggttg ggcacgcatc ttccgcatcg acgatgctgt ctccgtcacg
    21481 gtcgggcagt gggcagccgg gcttggccgg atcgggatga ccgcctgccg aaactttcgg
    21541 gcactgatcc accgagtcat acacgccatc gccatcggcg tcgctatcgg ggcagccgag
    21601 cttgccgggg tccggatgat cgcctttggg tttttgcgga cactggtctt tgctgtcgac
    21661 cacgccgtct gcatcgctgt ctttgtcggg acaaccgggc cgtttcggat cgggctcgct
    21721 gccctgggct tcggtagggc actgatcctg cgcatccaga acaccgtcgc catcgcggtc
    21781 cggaatcggg cagccgagct tgtttggatc gggccgatcg cccttgggta ccgtcgggca
    21841 ctgatccgtt tcatccggta caccgtcgcg gtcgcgatcc ggtggcggac agccgtttgt
    21901 gcgcggatct ttggtcgctg ggccgggatc gtttggacaa gcatctttgc gatcgatgat
    21961 tccgtcgcca tcgctgtcga cgcgcggtgg ttccggtttc ggcatcggcg cataggccac
    22021 ccgcaaaagc gcgcgcgcat cgggtgttcc cggctctcgc agcacgccga ctccaccagc
    22081 ggctccgacc tggacataac cggcaatgtt gtagtgaacc ccgagcagca cttcgaggct
    22141 ggtgtagtcg cgggcaaagg cgtggccgct caggatgtcg gtactgacga gcagctcggg
    22201 cccgatcgaa aagcgtcgta gcgtgtcagc atacgcagca gacaccccaa gctgcagcgc
    22261 tgatcccgtg gtgcttccgt cgggaatcat gtaattgccc agcgtcgctt cgggtcgata
    22321 caaaaaaccg agcgtcgccg accagcggat ctttttggcc agaccgccag cgacgatttt
    22381 gggcagcaca cggacaccag cgtcgctggt cagcatcggc agcgagctgt cgatcgcgcg
    22441 cagcggaatc cacaccgaag cgccggcgct tagcgaaaac gcgctgccgt cgggctgtcc
    22501 gaaaattcgt gccatcaccg aaatccgcgg gtcgccaacc gccatgccac tgaccggagc
    22561 caggtcgccg atttgttggc cgcgctccag cagtgtcacc ggcaccgacg ccgcgatcgt
    22621 cacccgatcg aggaacgagc ccgccaaatc gacattccca atcagctggt gattgatgat
    22681 cgaataggtc tggcgaaagc tgttgtcgac ggtgctatag ccgaaaacca gcggattgtg
    22741 cgcatagttt agggtgatac ccgccgcaaa ataccgcgtg gacgagtacc acggatgatc
    22801 gaccatgaac gaccactcac cgacggtggt tggttcgtat cggttgatct gaaagccgac
    22861 gttttgtgcg cgggccgggc ctggcagtcc caaaagcaaa aggagtgctg cccacagagc
    22921 ttgcgaaaat cggaaacgca cttcagcctc ccttacccga aaattcgacc gttccagtta
    22981 cgttgccgtc gcacaatgcg caggccgcag agcagaaatg ccatcgccca cagtgccgtg
    23041 ctgctcgtcc cggctgttcc ggttgtcatc gtgcagccaa atccaccacc ggctggacgc
    23101 gccacaagtg gggccggatt gtcgatggtg acgctggcgc tgttgttgtc caagttggga
    23161 tctgtcgtcg gcgcactgac tgtcgcggaa aacgcaagcg atgtggcacc gagcgccgga
    23221 atcagcgtga gcgcgatctg cggcgcctgc gtgatgggca gcgtcgctag cttgcagatc
    23281 atctgccgcg tcgtttgccg acacgaccag ttttgcgcgt cgatgttctg tactttgatt
    23341 cccgttggca gatcgatggc cacctgcacg ttgccgctca ggccgggccc caagttttgc
    23401 acatttacca caagctggaa cggatcgatt tcgtgcgcca cagacggcgt cgcgctcatc
    23461 gtcagcgaaa gatccgctat cgaacagtag ggtgcaccgg catacgggcc cagctgggtt
    23521 tgcacaatct gaccgagtga tccgtcgctg gcgccgtttc ttgggaatgc cagcatgtcg
    23581 gtcgatgcgg tcgtgccgcc gctgccaccg tttgcctgcg tcgtcaccgt cgtgctgggg
    23641 gacagcgtta cataaccgcc accaccacca ccacctgggc ccgcggct
//
LOCUS       DAORGN010000060        23588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_846_length_23588_cov_6.375218, whole genome shotgun sequence.
ACCESSION   DAORGN010000060 DAORGN010000000
VERSION     DAORGN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23588
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_23588_cov_6.375218"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..2325)
                     /locus_tag="PKL17_08830"
     CDS             complement(28..2325)
                     /locus_tag="PKL17_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK44872.1"
                     /translation="MGLGQYVFPRVAVHIASGRTVPVERLRAHCLWYPAVSLDGREVS
                     GHVLYFSQALEESLEVVLGLHSSWDWNPAAEVEQGEDDITFRLAAPVRASLHASVPQL
                     LKAIIDRVAQTMPQSKRRQVAAFDKLPTDKILTSCEEFLAQNKLHVLVPLFIGNLYQL
                     FTDATEALLPVSPFSLRRTPRLCFVLLSTDSLTDKRSSPWLRQAGELMALPVNVVFTP
                     QVAEFLDALPQLKMVRADSQLLSELLWTLHELTGGWPDLCDRFFEMLAQLPDLEALRR
                     RLWELAQLLRAAERDLEASQRLLDQSFAGRSDLPDLMLPGQSSPALMPLSPSLLTGMG
                     SSPMLPGGYEGLWSLLGQPVPFTWEEHGRAYLDGLVSWERKLAVPRSRAIELFLRRRL
                     ELLSSVQRSIGQPANQPNHTADQHPKIESPRVDSATVGAHPGLHTVRWLHVSDFHFSE
                     KHQTQGAGIVLESLLNTLDDMRRGGRGLDCIFVTGDIAQSGSDSEYRLAEEYLLRLCE
                     HSGVATQNVIMVPGNHDVARQRGFGLQRTLTQHDEAMHFFQPQAERWHLKKFEAWTSF
                     YNRFYKAGHFGKDAPRQCDPRLATAAAEIVKVRNLEIGILPLNTAWFAQADDDSGKLF
                     VGEPLLRAAISQVQKAKIKIALMHHPVQDLSEVERRNIEQLLQEHCHFVLRGHLHENE
                     AQWVSSPYRQTLVMAAGAAYQGRVVYQNRAMLCEVDVDSVGKTAKVRPYPIRYELTGH
                     DRWTLDTSVFPKSYPTYLETLSVNL"
     gene            2502..3176
                     /locus_tag="PKL17_08835"
     CDS             2502..3176
                     /locus_tag="PKL17_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44873.1"
                     /translation="MAAPVGKKSAARGAGKKTASSDANSDGNVRPPGGFKLADLVKPG
                     AVFSVWSSDYDASDLYPITIESIDPNGALVLSYGTPTGKATLRCEAASTEQGQGVVFL
                     SQFAAMTDGDTVSKNGKLTTATHSAPFLLSRALLAKLSAGEEVTLSVYSGSFAVKKVG
                     TAQATVRIHEKPVKVQVLHAQDTTETDFSPVDLWVLDHPVWPILLKLEFGGECELSLF
                     EIEPAK"
     gene            3223..5067
                     /locus_tag="PKL17_08840"
     CDS             3223..5067
                     /locus_tag="PKL17_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44874.1"
                     /translation="MPQKTRALPTVHPVLLRSSLALLCAIGIGCNKDPQIDGNPDGST
                     IPDGGDPTTSDLGMPVQNEICSLDRYCFRNPLPQGNGFNGVYVAASGEIVAVGDRGTI
                     MRYAGGVWNAEYSGSQSLLNAVWGPSPQDLWVVGEKGLILRYVSGTWKTQAPVTTFLL
                     NGIHGVDASNIWAVGTAGLILKWNGSTWSPQTSGTTTTLSAVWAADANNAFAVGSTGV
                     IVRFVGGTWNPQISGTTRTLLGVSGTDAMNAWAVGDLGTIVKWNGSAWSVQNSGSVSR
                     FYGAYAASLTSVWAVGTGGTILRFDGSAWSPQPSGDTNFFKAISGQGTSNIVAVSDAG
                     WVTRYGSGGFSPLTKGNTSTVYCAWGTDSQNFWAVGDQGTVLRYQNGVISVEPTGTTR
                     SLQGIWAASPQAAYAVGTAGTVVRYDGTKWSLVNSGSAANFYGVYGIDANNVWAVGDS
                     GTILRSQNGMTFAAQTSGVTGELDSIYAADATHAWAVGAGGVILFYNGTNWTPQSSGT
                     TVKLRSVHGTGANNVYAVGDAGTILRFDGSKWGSQTSGTSQVLARVFAQSPTSIYAVG
                     QSGTAVRSNGTTWNQEDSGASQFLFGLWGTQTNIFAVGGMGTMLEKKN"
     gene            5095..6492
                     /locus_tag="PKL17_08845"
     CDS             5095..6492
                     /locus_tag="PKL17_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNK44875.1"
                     /translation="MKPITAKRNRLLVFSTRFFAIGIGLGIGLGVSIAAHAATLQVGP
                     GKTYAKPCLAIAAAAAGDTVEIDAAGSYAGDTCAWSTDNLTVRGVGGRPKLDITGVQP
                     AQQKGLFTISAANATIENLEFSGAAISAASGNNGAGIRHQGTNLTVRGCVFHDNQNGI
                     LGSPAAGLTGQVLIENSEFYSNGAGDGFSHNMYLGNYATFILRHSYSHRGKVGHLVKS
                     RALVSQILYNRITDEMGGSASYEIDLPNGGTGVVLGNIIEQSATSQNPAMLAFGEESV
                     PSGYDSHLYVVNNTFLNTKGSGTFVSDATTTPAVLTNNIFWNGGTVSNQSTALLTSNF
                     VSAQSGDPGFVNVTGFDVHLLPSSPCIDKGTDPGSALSQSLSPVFQYVHPESQEARGT
                     SGTAIDIGAYEYGNRVSTDVGDSSGGGTTATGCSCQIGARAKTQGSGIGNQQGVLLGI
                     LSAWILRRKRSLRTS"
     gene            complement(6489..7019)
                     /locus_tag="PKL17_08850"
     CDS             complement(6489..7019)
                     /locus_tag="PKL17_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44876.1"
                     /translation="MNKAILAACAGVTFGLFLGGLLMSGGVSLRTCHRQKSVVTQEQS
                     YSVASQSQMTRHVVIEQRIIRSSDGVIHSETTRTETHMGAAMDSDETLTQAQTAYVNG
                     DYAQAITLAKTAAKDSPIRASRIIGSAACQTKNLALVEESYRKLDAPSRQYLVYVCQR
                     NGISARGNRFVLTSDR"
     gene            7154..8056
                     /locus_tag="PKL17_08855"
     CDS             7154..8056
                     /locus_tag="PKL17_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNK44877.1"
                     /translation="MEPRSVLSDWLWIARYHIVLVAMTAQVVFGFLFSGRYLLGLSLV
                     VGLDWFLINLLNRITDVAEDSHNQVPGTARVAQNKTALTVLATVLLVSSFAVTYVVWP
                     QLTPWRVAVQLIGMGYNFKIVPTLHGLSRFKEMYFFKNFGSSVLFVLTCFAYPIVVAG
                     HENMGVAAICMLALFFVLFETTYEILYDFRDLDGDALLHVPTYPVVHGPDIAEKIMRG
                     LLLSSGLVLGIGAVFGVLGVRELLFAVAPLVQFVFYRARLVRGLTSQDCLWLTHVGSA
                     QLVLFLVGTALWKSAGLPENIFLR"
     gene            complement(8048..10189)
                     /locus_tag="PKL17_08860"
     CDS             complement(8048..10189)
                     /locus_tag="PKL17_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNK44878.1"
                     /translation="MLALRDCATFAAAGPKACRLSRALAMGISVPDGVVILPSEPLPS
                     FESLQEALSRLWKSPWESATQFVVRSSALAEDLAGQSAAGLFLSRTHVPIDDVVDAIR
                     SVRESGETELIKLALGQTVPVAVLIQRQVDAERLGVLYRSQLGELRCEEREAGAPEWA
                     DVTVIRYGAGEVNQLSEGAAQLATLLSAEEHTAEPVAIYAEYAVTKSGLVTFLQARPA
                     PPTDSQSGFVIDDDREHSFRLDQEHNPDPLSAAQQGLVDAVADLVPWLRQRVVRGYLY
                     YAQVAGKKPQVMQRQTREVFTTTILPTCEAMLGPAEKRLLAADGALRADLLTDALGLD
                     LGLGEALSIYRSIYDQYVGLLGPAIKWARTQLDELLLANLGEPLGQHGDLLAGIGGAQ
                     TERVQLLWELGRAGCPQAALRRVLARFGAFSSGWDVRVPCDDEQPDAIADYARHLFGL
                     PETPEQKQKAALLRYQQAVQTLESRLPRAARSALQSLVLLVRDAMRVAEDDDLLFFRA
                     QRLVRWALRARGAKLLATGRLADPQLVFDLPWRPWRAGQDPFDYPADVDLSRLARAAR
                     HDRLAAQKLLPPQRIVAGRPEWDLPDGRTLSGSGISATQSVVTGRALVVPSLDDPEAS
                     LRIVLSQLSPDTILVLPTLLPSWAPAVWGALAVVTDCGGALSHGAILAQERGIVAVLG
                     TKLATRLVQTGQRLLVDGSRGLVVLSPSPSA"
     gene            complement(10199..12163)
                     /gene="acs"
                     /locus_tag="PKL17_08865"
     CDS             complement(10199..12163)
                     /gene="acs"
                     /locus_tag="PKL17_08865"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015198914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNK44879.1"
                     /translation="MKTIDSVLTEKRSFPPSGTFVEQAHLDAEGLRLLREKAQQNPDG
                     FWLEQAAHITWRTPPTQGLDDKDHPFVRWFADGQLNITESCLDRHLSTHRRNKAALIW
                     EGEPGDQRVLTYYELHREVCRAATALRKLGIRMGDRVIVYMPMVPELVIAVLACARIG
                     ATHSVIFGGFSAEAIRDRINDAGAVAVVTADGGYRRGKVVPLKHNVDEALVHCPSVKH
                     VVVVSRTGQDISMHPGRDHFWTDLVRSEAGVCEPAIVPSEHPLFILYTSGTTGKPKGV
                     VHSTGGYATQVAFTTKYVFDLRDEDTYFCTADVGWVTGHSYIVYGPLINGATVVIYEG
                     APDAPHRGRFWEIIDKWGVTIFYTAPTAIRAFMRWGDAHPRSHRLDTLRLLGTVGEPI
                     NPAAWVWYHETIGRGRCPIVDTWWQTETGGIMVAPLPGATVAKPGSCTQPLPGIAVEV
                     VHKDGSRCGKNEGGYLVLTRSWPGQLRTVYGDPERYRKTYFGDLKVDGRPVYFTGDGA
                     RFDEDGYLWVMGRVDDVLNVSGHRIGTAEVESALVGHPHVAEAAVVGRPDDIRGQAIV
                     AFVTLRGGHAGSEQMAGILKEHVVHEIGAIARPEEIRFTDALPKTRSGKIMRRLLREI
                     ATTGRAMGDVTTLEDMAVLQRLSTSDSDEE"
     gene            12226..13305
                     /locus_tag="PKL17_08870"
     CDS             12226..13305
                     /locus_tag="PKL17_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44880.1"
                     /translation="MLGVETLLLPVSFLDWLRTRLFSLLGPLGQPIVRRPELRVALRG
                     SVAVLLALTGTILLPLWILALGPLILGVPHLLSDARYLVVRPGHQRSPRLVLAVGIPL
                     VICGLGVGMPAGLAALAGVFLVSDGALWRRCIGLSLVGALALWGYAAHSFYPHELWLA
                     HLHNPIGVLIWFLWRPRHERLHLIPLLLYVACATVIGLGVVHALPFGLSAAPHSDGSA
                     LEFFRDTLAPEFSETWGTRLVLLYCYAQMVHYSVWLQLVPEEDRPRPTPRSFRESLRV
                     LFADLSWPLLLLAMLIALSLLLFALRDVWAARTAYFRLATFHGYLELCALGLYFVKGQ
                     RPQKARRLVETAQGFLPVSAAAAHR"
     gene            13359..13997
                     /locus_tag="PKL17_08875"
     CDS             13359..13997
                     /locus_tag="PKL17_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44881.1"
                     /translation="MKWTFAAMALGGVLLAGFSAHAEGDAEAAPVSPGQVRHVPATAE
                     TAEEASPKTAPRPPTVRSAAAKPDGTAATVKPMLPADYRPHGETGAAAKETGAKDAAT
                     KPTSKPAAGDKAKDTTAKKRIPLAPTEAELKREIALRNGGPDAPATDNRPTSEKLADK
                     ATSIPNQRREWPTTVNNTAGPTSKAGPGSAQDRKGTMIEATGPQQGVIRNRW"
     gene            14010..14786
                     /locus_tag="PKL17_08880"
     CDS             14010..14786
                     /locus_tag="PKL17_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNK44882.1"
                     /translation="MSSKPLRFVTLPISHYCEKVRWALERQGVPFIEEGHGPVLHAFS
                     TMPLTGGKSRTVPILVDENLSPRRVLIDSTDCLRHLADHYGASWLYAHPEAAALEDEL
                     DDKLGPATRRFAYFYLLPERATADVLTQNVPGWEASFARAMFPLIRRGMAKAMNISPE
                     GAQRSLGQLRELMERYGARLADGRRYLCGDTLTAADLTFAALASPVIFPSGYAKITMP
                     DITAMPTPLREEVQRQRQSPAGQFVLRLYAEERAKTIRSA"
     gene            complement(14764..15501)
                     /locus_tag="PKL17_08885"
     CDS             complement(14764..15501)
                     /locus_tag="PKL17_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44883.1"
                     /translation="MMQNRSLLRAFSSRVGASASGLAVAALCVLATPTAQAQVLQDAN
                     GKPFVPPPPLPPPSGQPVPRPASVVTQPMQPVSPPPVMPADVLPGSSMCADVHMEMQQ
                     LLSALLEHRRTAETVAIRQRNRSLIAGGATLFASGYLAAVIAGSVYLQSSQGSSFARD
                     VSTERSASGTLMIPVVGPLVSSLIWRDPIWAINWSLVDGVAQMGGVAMMVVGIVSNRR
                     LPAPLVGLQLAPVRTSTQTGLTLSGSF"
     gene            complement(15589..17991)
                     /locus_tag="PKL17_08890"
     CDS             complement(15589..17991)
                     /locus_tag="PKL17_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK44884.1"
                     /translation="MKKPLLVSALILGLPSWVLAQEVLPPARRSASALDPAYDVLLPD
                     PEPVVDNATKTDVDRGSGNTDIDIADRVLSAAKQVTTVQEAPSIVTVVRADEIQARGY
                     RNITQVLETIPGWLSSSNGGSLLSIPLVRGTAQAALLLRDGVSMFEPVLNVGTYNRGL
                     PLETVKSVEVVTGPGGVLWGANSFLGIVNLISKDADDINGLEASAGYGDGPGSPQDFR
                     AYVLFGKSFRFKSGPKLSIVQHVSYETYVASRFNVLASLYRNAAPSPSGPHLYSTNLD
                     TNPERSWIVNVDGKISYGPVTLSYSLPFALMNTSLSFANNPTSGAWDTGSNAPAGKPV
                     RNYLQSFDRFVTLSYKGRFLKDRLGLDVKSYAIEFVRDLNAIILPGSARNPNGTTFDT
                     QIRSYRVGLTIDGDASLPLRNRLLWGGDLFYEWVPELYVNFPQSNPQDLPIGCPLEKS
                     GTAMPVFKAGCKLPFLFGTNRLVTALYVSDTFRPVSSLTLDAGVRLQGGFGKAQYNGG
                     NLGNSGTVLGSASLVWNFYRDMHVKANYASGFRPPVFNNTDSNGAAVQFGGSHFLSNE
                     KSQAIQAEWNAKLLRNIGPIRALQLRADYAYTDLNGLIVISNGSYTNRRTDANGALAD
                     QPGQDSRNFNSHRQIHSVESAARLYIGDHTLSANYTFLHITTNDRGVLRSMPQHSFNL
                     GWVLSVIPGWLETNGTLMVVGSYDDPNRLRTLSLADGSTIANFSDLTFDKLPPQAILN
                     LGARAHFFKNRLWASLNFYNVLNQTYYYPDPFYDLAPTLEVSPTPAPGWSTFFQIGGK
                     PF"
     gene            complement(17988..19025)
                     /locus_tag="PKL17_08895"
     CDS             complement(17988..19025)
                     /locus_tag="PKL17_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44885.1"
                     /translation="MIRRALVLPFFTCAVSLVASSARADRSIDVVMTPPAMDAYGLQV
                     VDRAQTPQQFEFGMSANVGWAGKPLRLTLNDTNMGRQEALFAVIENQVTLDVGFYFGL
                     WDFLSLSAMVPVGVNYYDANAVGDPTVSVLPTPPATTGSTVSTGIYAGQPRQNVVLST
                     VGVRDPRFAVKARFYGGKYFEIGTLLEVTAPLGDSNSFLGDKNFTFRPKLLLGGLFGR
                     LHVLGSFGAVVRQSSQLFEPNPTTAQASDPPLRLTVGHELTWGGALTVRLHRVIGLGI
                     EAVGTIPVTGDKTNMTGTALGSIYILPTEKLRLTLTGGGGLLGSDPRNPTGRVMVGMA
                     YSMLPRAGGLL"
     gene            complement(19022..20047)
                     /locus_tag="PKL17_08900"
     CDS             complement(19022..20047)
                     /locus_tag="PKL17_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44886.1"
                     /translation="MSIAWPLPPQPCVATGVPAGCTPPPVVPTLPFPSHHVEMYAYYA
                     GNPKPERIIANVGGQTDPQAFSGFQVLPVVDPSEPCAIRGLDRDDGGCGRGSLSSSTC
                     GAQYYTQKAQRATDDEALSDVTLAGRDQLLLQVGKVVSKLTPFPSTNADLNLATKGRA
                     ATTMLVLVQHNPDNANEPRRSGALMAITPTTAEDDAESDRRLNVCKAYRDGGHPNFYV
                     GNPKQYTKPLSGVLFGMLLYQTGAGTAAPDLPTQNFSGITFSVPTNMKDLIEIQIRIE
                     PDNDKLPNAIYYPVIQGRRLPDSAAGRGAIKFAMYANTNGPGLPTNFSALVGSASVET
                     QLDARLQ"
     gene            complement(20187..21761)
                     /locus_tag="PKL17_08905"
     CDS             complement(20187..21761)
                     /locus_tag="PKL17_08905"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNK44887.1"
                     /translation="MLRRGVVWLLSVLLVFWFAFDWAAARPGGGQSYRSSSSSRSSSS
                     SSRSYSSGSSSSRSYSSGSSSSGSSRSYGSSSSSGSSSYGSSSGSSSGSTTVVYTGSS
                     SSSGSGWVVVVVIILVIILLIWLAGRNKKPERAKLDVDESLAKAGLADLRQHDPEFEP
                     MSFLARTKTVVGKVNEAWLSGNMGPARRVISDGVYVRFSTQLELLKSQGLRNMMADWR
                     VVSADIMAAVADPNWDTVHVKIVGEARDRDVSVSLSPSEALSQTKKAELGQYHEVWSF
                     VRRRGKHSKKGIPALEGKCPSCGADLPLGEVVKCEYCKALVNSGEHDWVLAEITQPEE
                     WRAEPAVGDISGLDELRERDPQVSRQEIEDRASVMFWKWIEAKATGKLDKLNRFCVKP
                     PTTETDRSELSLEPSKLSQVAVGSAELESVLTGGEQDYAYVEIMWSASVDGREPMTFT
                     HVLALGRSATAKSRRGMSSLDCPECGGALAGSDAVTCQYCGTKLAGGKHEWALFAVQG
                     GEADGAGDEGDEEDND"
     gene            complement(21874..>23588)
                     /locus_tag="PKL17_08910"
     CDS             complement(21874..>23588)
                     /locus_tag="PKL17_08910"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK44888.1"
                     /translation="RKEGNTIRLTERNVSRRHARITRQGNGLFIEDLLSYNGIKVNGD
                     RIQGKVAVGDGDRVQIGDYQVSVKLDKGAAKVQVPGQGPQTTPVNRAEPTLMSGTPAI
                     PAPGAATLAQPVQAPVVKVPEKPARLIVVSTNFARQEFQLEKATAVIGRTDDNDVVLN
                     HRSISRHHAKIVREGDKFQIMDMQSANGVRVNGEEYGKVELRKGDKVDLGHVRLVFIP
                     PGQDIPKEQIDAMIVDRDQRGTPVVPIILTLVLLAAVGGGAYWFLTQPPKVDVDAEAA
                     KTLIDVESDLAAKRWQDVLDKTGKIIGNNAIAIRTRDAATAKRTVAEKESKNKQIYDR
                     FAGAAGSGNYDAALKTYREIPEDSVYQKSAKEDYDKIFPLFVESHLKNASDARAAGNC
                     GEARSQVQMVLDVDPKQVKALAAKDQPCGGSGGSSSVSNDGGNAGTKEHHRKDKEKGK
                     VADNEKPEKPEKTEKPEKAEKPEKVEAPAAGGDAEQVLTDAQSEFVNGNYDKAISLAR
                     SVAKVSTNRAWRIIGAAACRNKDLKLVGDAYRKLDNAARQYLIYVCQREGIVQNGSSF
                     KLSE"
BASE COUNT         4662 a         7385 c         6987 g         4554 t
ORIGIN      
        1 gagtccgggc gaaagctacc gatagcgcta caggttgaca gacagggttt ccagataggt
       61 tggatagctc ttgggaaaca cactggtgtc gagcgtccag cgatcgtgtc cggtcagctc
      121 ataacggatc ggatacggtc gcactttcgc ggtctttcca acggaatcga cgtcgacttc
      181 gcagagcatc gcgcgatttt gatacacgac gcgcccctga tacgccgccc ccgctgccat
      241 caccagcgtc tgccgatacg gactcgatac ccactgcgct tcgttttcgt gcaggtgtcc
      301 gcgcagcaca aagtgacagt gttcttgcag aagctgttcg atgttgcggc gttccacttc
      361 cgacaagtcc tgcaccgggt gatgcatcag cgcgatcttg atcttggctt tttgcacctg
      421 ggaaatcgcg gcccgcaaaa ggggttcccc gacgaacaac ttgccactgt cgtcatcggc
      481 ctgcgcaaac cacgcagtgt tcagcggcaa aatcccaatc tcaagattcc tgactttgac
      541 gatttccgcc gctgcggtcg ccagacgagg atcacactgc cgcggcgcgt ctttgccaaa
      601 atgaccggct ttgtaaaacc gattgtagaa gctcgtccat gcctcgaatt ttttcaggtg
      661 ccagcgctct gcctgcggct ggaaaaaatg catcgcttcg tcgtgctggg tcagcgtccg
      721 ctgaagccca aagccgcgct gccgagcgac atcgtgattg cccggcacca tgatcacgtt
      781 ctgcgtcgcg acaccgctgt gttcacacag gcgcagaaga tactcttccg ccaagcggta
      841 ttcgctgtct gatccgctct gcgcgatgtc cccggtcacg aaaatgcagt cgagcccgcg
      901 cccgccccgc cgcatgtcat ccagcgtatt gagcagcgac tcgagcacaa tcccagcgcc
      961 ctgtgtctgg tgcttttccg aaaaatgaaa atccgacaca tgcagccagc gcacggtatg
     1021 cagacccgga tgcgcaccca ccgtcgcaga gtccacccgt ggtgattcga ttttgggatg
     1081 ctgatccgct gtgtgattcg gctgattcgc aggttgtccg atgctgcgct gtaccgagct
     1141 aagcagctcc agccgtcgac gcaaaaacag ctcgatggca cgactgcgcg gcaccgccag
     1201 cttgcgttcc cagctcacca gcccgtcgag atacgcgcgc ccgtgttctt cccacgtaaa
     1261 cggaaccggc tgtccgagca aagaccacag cccttcatag ccgcccggca gcataggact
     1321 tgatcccatt ccggttagca gcgacgggga tagcggcata agcgccggcg agctttgtcc
     1381 cggcagcatc agatcgggca gatcgctgcg accggcaaac gattgatcga gcaggcgctg
     1441 gctcgcttcc agatcgcgct ctgcggcgcg caaaagctgc gccagttccc acagtcgccg
     1501 ccggagcgct tccagatcgg gaagctgcgc cagcatttca aagaagcggt cgcacagatc
     1561 gggccagccg ccggtcagct cgtgcagcgt ccacaacagc tcgctgagca gctgcgaatc
     1621 cgcgcgcacc atcttgagct gcggcagcgc gtcgagaaac tcggcgactt gcggcgtaaa
     1681 caccacgttg accggcagcg ccatcagctc gccggcctgc cgaagccacg gactcgatcg
     1741 cttgtcggtc aggctatccg tcgacaaaag cacaaaacag agccgcggcg tgcgtcgcag
     1801 cgaaaacggc gacaccggca agagcgcttc ggtcgcgtcg gtgaaaagct gatacaagtt
     1861 gccgataaac agcggaacca ggacgtgcag cttgttctgc gccaaaaact cttcgcacga
     1921 ggtgaggatc ttgtccgtcg gcagcttgtc gaacgcggcg acttggcgac gtttcgactg
     1981 cggcatcgtc tgagccaccc gatcgatgat cgctttcaaa agctgcggca ccgacgcatg
     2041 cagcgacgct cgaaccggcg cggcgagtcg aaaggtgatg tcatcttccc cttgctcgac
     2101 ttccgccgcc ggattccagt cccacgacga atgcagcccg agcacgacct ccaaggactc
     2161 ttcgagcgct tgcgagaagt acagcacatg cccggacacc tcacgcccgt cgaggctcac
     2221 cgctggatac cacaagcagt gcgcccgcag cctttccacg ggaaccgtcc gccccgaggc
     2281 gatgtgaacc gccacccgtg gaaacacgta ctgtcctaga cccaagctga cctccttgcc
     2341 gtaacgcgaa gcatacgacg actttccgac acttgcccgt ttgccgattc cgcctcgctg
     2401 acagcgcgaa gtgggcgacg ataacacgcg caggcccgcc aagcccgcgc aagcagacgc
     2461 aacctgtggt atgcacgcag cctgcgagga ggatttttgg gatggcggca cctgtgggga
     2521 agaagtcggc agcacgcgga gcaggcaaaa aaacagcctc aagcgacgcg aattccgacg
     2581 gaaacgtgcg accacctggc ggtttcaagc tggccgatct ggtgaagccg ggcgcggtat
     2641 tttcggtgtg gtcgtcagac tacgacgcga gcgacttgta tccgatcacg atcgaatcga
     2701 tcgatccaaa tggcgcgctc gtgctgtcgt atggaacgcc aaccggcaaa gccacgctgc
     2761 gctgcgaagc cgcgtcgacc gagcaaggcc aaggcgtcgt gtttctgtcg caatttgctg
     2821 cgatgaccga cggcgatacg gtgtcaaaga acggcaaact gaccacggcc acgcacagtg
     2881 cgccgttttt gctaagccgc gcgctgctcg ccaagctgtc tgcgggggaa gaagtcacgc
     2941 tgtcggtcta ttcggggtcg tttgcggtca aaaaagtcgg gaccgcgcag gcgacggttc
     3001 gcatccatga aaagccggtc aaagttcagg tgctgcacgc gcaagacacg acagaaacgg
     3061 acttttcgcc ggtcgatctg tgggtgctcg atcatccggt ctggccgatc ctgctgaagc
     3121 tggagtttgg cggcgagtgc gagctgtctc tgttcgagat tgaaccagcg aaatagcgtc
     3181 cgctgttgat gcgcaagcgg ttcgccgcgg tacactggga agatgccgca aaagactcga
     3241 gcacttccca cggtacaccc ggtgctgcta cgttcctcgc ttgcactgct ctgtgcgatt
     3301 ggtatcggct gtaacaaaga tccgcagatc gatggaaatc ccgatgggag tacgattccc
     3361 gacggtggag atcccacgac aagcgatctc gggatgccgg tccagaacga aatttgcagc
     3421 ctggataggt actgttttcg caatccgctg ccacaaggga atgggtttaa cggcgtctac
     3481 gtagctgcca gtggagagat cgttgccgtc ggagatcgcg gcacgatcat gcgctacgca
     3541 ggtggcgtct ggaacgccga atacagcggt tcacagagtc tcttaaatgc agtgtggggg
     3601 cccagtccgc aagacctctg ggtcgtcggt gaaaagggac tcatcctccg gtatgtaagc
     3661 ggaacgtgga aaacgcaggc cccggtcacg acatttttgc taaacggaat ccacggtgtc
     3721 gacgcaagca acatctgggc ggtcggaacc gcgggtctga ttctgaaatg gaacggcagc
     3781 acctggagcc cgcagacatc cggaaccacg accacgctgt cggcggtttg ggctgctgat
     3841 gcaaacaatg cttttgcggt cggatcaacc ggcgtcatcg ttcgctttgt cggtggcacc
     3901 tggaatccgc agataagtgg aacaacccgc acgctgcttg gcgtcagcgg aaccgatgcc
     3961 atgaacgctt gggccgtcgg tgatctgggc acgattgtga aatggaacgg cagcgcgtgg
     4021 agtgtccaaa acagcggcag tgtcagtcgt ttttacggtg catatgcggc gtctctcacc
     4081 agcgtgtggg cggttggcac gggcggaacc atcctgcgct ttgatggcag cgcctggagt
     4141 ccgcagccaa gtggcgacac caattttttc aaagcgataa gtggccaagg gacaagcaac
     4201 attgtggcgg tcagcgacgc gggatgggtc actcggtatg gcagcggagg tttttcgccg
     4261 ctcaccaaag ggaataccag cacggtgtac tgcgcgtggg gaaccgattc ccaaaacttc
     4321 tgggcagtcg gtgatcaggg caccgtactg cgctatcaaa acggcgtcat ttccgtcgag
     4381 ccaaccggca cgacgcgcag tctgcaagga atctgggccg catcaccgca ggccgcttat
     4441 gcggtcggaa ccgccggcac cgtggttcgt tacgacggca ccaagtggtc gcttgtcaac
     4501 agcggcagcg ccgcgaactt ttacggcgtg tacggaatcg atgccaacaa cgtctgggcg
     4561 gtgggggatt ccggaacgat tctgcgcagc caaaacggaa tgacgtttgc cgcccaaaca
     4621 agcggtgtca caggagagct ggactcgatc tacgctgccg atgcgaccca tgcttgggct
     4681 gttggtgcgg gcggcgtcat tctgttttac aacggcacaa actggactcc gcagagctcc
     4741 ggcaccacgg tcaagctgcg cagcgtccac ggaaccgggg caaacaatgt gtacgcagtc
     4801 ggagacgccg gcaccatcct gcgtttcgac ggcagcaagt ggggaagcca gacttcggga
     4861 accagccaag tcctggcgcg cgtctttgca cagagcccaa ccagcatcta cgcggtggga
     4921 caaagcggaa ccgcggtgcg ctccaatggg acaacgtgga accaggaaga cagcggggcg
     4981 agccagttcc tattcggtct gtggggcacc cagacaaaca tatttgccgt cggtggaatg
     5041 ggtacgatgc tcgaaaagaa gaactaacct acgttcccag tggagcttga cctgatgaaa
     5101 ccgatcacag ccaaacggaa tcgattgctg gttttttcca cccgtttttt cgcaatagga
     5161 atcggcttgg gaatcggctt gggagtctct atcgctgcgc acgcagcgac gctgcaagtg
     5221 ggaccgggaa agacctatgc caagccttgc ctggcgattg cggcggcagc agcgggagac
     5281 actgttgaga tcgacgcggc aggttcgtat gcgggcgata cctgcgcatg gagcaccgac
     5341 aacctgaccg ttcgcggcgt gggaggccgg cccaagctcg acatcacggg cgtccagccc
     5401 gcccagcaga agggactctt caccatttct gctgcgaacg cgacgatcga aaacctcgaa
     5461 ttttccggcg cggcgatttc ggctgcatcg ggaaacaatg gcgcgggaat ccgccaccag
     5521 ggaaccaacc tgaccgtgcg cggctgcgtg ttccacgaca accagaacgg aattttggga
     5581 tcaccggcgg cggggctgac agggcaagtg ctgatcgaaa actccgagtt ttacagcaac
     5641 ggtgccggcg acggattttc ccacaacatg tatctgggga actacgcgac gttcatcctg
     5701 cgccattcct attcccatcg cggcaaagtc ggacaccttg tcaagagccg cgcgctcgtt
     5761 tcgcaaattc tgtacaaccg catcaccgac gaaatgggtg gctctgcaag ctatgaaatc
     5821 gatcttccca atggtggcac cggtgtcgtt cttggcaaca tcatcgaaca gagtgcgacc
     5881 agccaaaatc cggcgatgct cgcgtttggg gaagagtctg tcccatctgg ttacgactcc
     5941 catttgtatg ttgtcaacaa caccttccta aacaccaagg gttcgggaac gtttgtgtcc
     6001 gatgcgacga caacaccggc agtgctaacc aacaacattt tctggaatgg cggcacggta
     6061 tcgaaccaaa gcacagcact tctgaccagc aacttcgtga gcgcgcaaag tggagatccg
     6121 ggctttgtca atgtgacagg cttcgatgtc cacctcttgc caagctcgcc gtgcatcgac
     6181 aaaggaaccg accccggcag cgcgctttcc cagagcctgt ctccggtgtt tcagtacgtg
     6241 catcccgaaa gccaggaagc gcgcggaacc agcggaaccg ccatcgacat cggtgcgtat
     6301 gaatatggga atcgggtttc taccgacgtt ggagacagca gcggaggcgg cacaaccgcg
     6361 accggctgta gctgtcaaat cggagcccgc gccaagacgc aagggagtgg tattgggaat
     6421 cagcaaggcg tcctgctggg aatcctaagc gcttggattt tgcgtcggaa acgatcgctt
     6481 cgcacaagct aacgatcgct ggtcagcaca aatcggttgc cgcgcgcgga gattccgttg
     6541 cgctgacaca catagaccag atactgccgg ctcggcgcgt ccagtttccg gtatgactct
     6601 tcgacgagcg cgaggttttt ggtctgacag gctgccgatc cgatgatgcg actggcgcgg
     6661 atcggactgt cttttgccgc cgttttggcc agcgtgatgg cctgggcgta gtccccgttt
     6721 acataggcgg tctgcgcctg ggtcagcgtc tcatcgctgt ccatcgccgc gcccatgtgc
     6781 gtctctgttc gcgtcgtctc gctgtggatc acgccgtcgc tgctgcggat gatccgctgt
     6841 tcgatgacca catgccgcgt catctgtgac tgtgacgcga ccgaatagga ctgttcctgg
     6901 gtcacgacac tcttttgacg atgacaagtc cggagggaaa cgcccccgct catcagcagt
     6961 ccgcccagaa acagtccgaa cgtgacaccc gcacaagcgg ccaagatcgc tttgttcata
     7021 ctggcaagcg taacgtaggc gatagcgacg gaaaagagcg cgaagctagg tctgcaggat
     7081 gtttgggaat tgccacggcg acggcgcgaa aagcgctgca ctgcacgccc cggtttggta
     7141 gaagcaaagc accatggaac cgcgaagcgt gctttcggat tggctgtgga ttgctcgcta
     7201 tcacatcgtg ctggtggcga tgacagcgca ggtggtgttt ggctttttgt tttcgggccg
     7261 ttacttgctt ggcctgtctt tggtggtcgg gctcgactgg tttttgatca acctgctaaa
     7321 ccgcatcacc gacgtggcgg aggattccca caaccaagtt ccggggaccg ccagggtggc
     7381 gcagaacaag accgcgctga cagttttggc gacggttttg ctcgtctcgt cgtttgcggt
     7441 gacgtatgtg gtgtggccgc agcttacgcc gtggcgcgtc gcggttcagc ttatcgggat
     7501 gggctacaac ttcaaaattg tcccgacgct gcacggcctg tcgaggttta aggaaatgta
     7561 ctttttcaaa aacttcggct cgtcggtgct gtttgtgctg acgtgctttg cgtatccgat
     7621 tgtcgtggcg gggcacgaaa acatgggcgt tgcggccatt tgcatgttgg cgctgttttt
     7681 cgtgctgttt gaaacgacct acgaaattct ctatgacttt cgcgacctgg atggcgacgc
     7741 gctgctgcat gtgccgacgt atccggtcgt gcatggcccc gacatcgcag aaaagatcat
     7801 gcgcgggctg cttttgtcgt cggggcttgt gctgggaatc ggcgcagtct tcggtgttct
     7861 gggtgtgcgt gagctgctgt ttgccgtcgc gccgctggtt cagtttgtgt tctatcgcgc
     7921 gcggcttgtg cgtggactca ccagccagga ctgcctgtgg ctgacgcatg tggggagcgc
     7981 ccagcttgtg ctgtttttgg tcggaaccgc gctgtggaag agcgccgggc tgccggaaaa
     8041 catttttcta cgctgacgga cttggcgaca gcacgaccag accgcgcgat ccatcgacaa
     8101 gcagtcgctg accggtctgg acaagccgcg tcgccagttt ggttcccagc accgccacaa
     8161 tgccgcgctc ctgcgccaag atcgctccgt gtgacagcgc gcccccacag tctgtgacca
     8221 ccgccaatgc cccccaaact gccggggccc acgacggaag cagcgtcggc agcaccaaga
     8281 tcgtatcggg cgaaagctgg gacagcacga tccgcagcga cgcttcggga tcgtccagac
     8341 tgggcaccac caacgcgcga ccagtcacca cactctgcgt tgccgagatt cccgatccgc
     8401 tgagcgtccg tccatccggc agatcccatt ccggacgacc tgcgacaatc cgctgtggcg
     8461 gcaagagctt ctgcgcagcc agtcgatcgt gtcgcgccgc gcgtgccaga cgcgacaaat
     8521 cgacatcggc agggtaatca aacgggtctt gtcccgcccg ccacggacgc cacggcagat
     8581 cgaacacaag ctgcggatcg gcgagccgtc ccgtcgccaa aagtttggca ccgcgcgcgc
     8641 gcagcgccca cctaacaagt cgctgggccc ggaaaaacag caggtcgtcg tcttcggcca
     8701 cccgcatcgc atcgcgcacc agcagcacca gggattgcag cgcactgcgg gccgcacgcg
     8761 gcagccgaga ctccagcgtc tgcaccgcct gctgatagcg gagcagcgcc gccttttgtt
     8821 tttgctccgg tgtttccggc aggccaaaca ggtgccgcgc gtaatcggca atcgcatcgg
     8881 gctgctcgtc atcgcacgga acccgcacat cccagccgct tgaaaacgcg ccaaaacgcg
     8941 ccaggacgcg ccgcagcgcc gcttgcggac atccagcccg cccaagctcc cacagaagct
     9001 gtacgcgctc ggtctgcgcg ccgccaatcc ccgcgagcag atccccatgc tggcccagcg
     9061 gctcacccag gtttgccagc agcagctcat cgagctgcgt ccgtgcccac ttgatggccg
     9121 gcccgagcag accgacgtac tggtcataga tgctgcgata aatcgacagc gcctcgccga
     9181 gccccagatc aagtcccaag gcatcggtca aaagatccgc ccggagcgca ccatctgctg
     9241 cgagcagtcg cttttccgcc ggcccgagca tcgcctcgca ggtcggcaaa atcgtcgtcg
     9301 tgaagacttc gcgcgtctgg cgctgcatca cctgcggttt tttgcccgcg acctgggcgt
     9361 aatacagata gccacggacc actcgctggc gcagccacgg cacaagatcg gcgacagcgt
     9421 cgaccaaacc ttgctgcgcc gccgacagtg ggtcgggatt gtgttcctga tccagccgaa
     9481 agctgtgctc gcggtcgtca tcaatgacaa agccggactg cgagtcggtg ggcggggccg
     9541 gtcgcgcttg caaaaacgtc accagaccgc tttttgtcac cgcgtattcg gcgtagatcg
     9601 ccacgggctc ggcggtgtgc tcttccgccg aaagcagcgt cgcaagctgc gcggcacctt
     9661 cggacagttg attcacctcg cctgcgccat aacggatcac ggtcacgtcg gcccactcgg
     9721 gggctccggc ctcgcgctcc tcgcagcgca gctcgccaag ctggctgcgg tacagcaccc
     9781 cgagccgctc cgcatcgacc tgacgctgaa tcagcaccgc gacgggaacc gtttgcccaa
     9841 gcgccagctt gataagctcg gtttcaccac tttcgcgcac actgcgaatc gcatctacca
     9901 cgtcgtcgat tggcacatgg gtacgcgaca aaaacagccc ggcggcgctc tgccccgcaa
     9961 gatcctcggc cagcgccgac gagcggacca caaactgcgt cgccgattcc cacggacttt
    10021 tccacagccg cgagagcgct tcttgcagac tttcaaacga cggcagcggt tccgacggca
    10081 gaatcaccac cccatcggga actgaaattc ccatcgccag ggcgcgcgac agccggcatg
    10141 ccttcggtcc cgccgcagca aacgtcgcac agtctcgcag agccagcatg agcttgggct
    10201 attcttcgtc gctgtctgac gtggacagtc gctgcaacac cgccatgtct tccagcgtcg
    10261 tcacgtcacc catcgcgcgt cccgtggtcg caatttcgcg caaaagccgt cgcatgatct
    10321 ttcccgatcg cgtcttcggt agcgcgtcgg tgaagcgaat ctcctcgggc cgggcaatcg
    10381 caccgatttc gtgcacgacg tgctctttca aaatgcccgc catctgttcg cttcccgcgt
    10441 gtccgccgcg cagcgtcaca aatgcgacga tggcttggcc tctgatgtca tcgggacggc
    10501 ccaccaccgc ggcttcagcg acgtgaggat gtccaaccag cgcgctttcg acttcggcgg
    10561 tgccgatgcg atggcccgat acgttgagca cgtcgtcgac ccgacccatc acccacaaat
    10621 aaccgtcttc atcaaagcgc gccccatcgc cggtgaaata caccggacgg ccatcgactt
    10681 tgaggtcgcc aaaataggtt ttgcgatagc gttcggggtc gccgtagacg gtacgaagct
    10741 gtccgggcca cgagcgcgtc aataccaagt agccgccttc gtttttgccg cagcgactgc
    10801 cgtctttgtg caccacttca accgcaattc ccggcagcgg ctgcgtacaa gagccaggct
    10861 ttgcgacggt ggctcccggc agcggcgcga ccatgatgcc gcccgtttcg gtctgccacc
    10921 aggtgtcgac gatcggacag cgaccgcgcc caatcgtttc gtgataccag acccacgcgg
    10981 cgggattgat cggttctccg acggtgccaa gcagtcgcaa agtgtccagt ctgtggctgc
    11041 gcggatgcgc gtcaccccag cgcatgaacg cgcgaattgc cgtcggtgcg gtatagaaaa
    11101 tcgtcacgcc ccacttgtcg atgatttccc aaaaacgacc gcgatgcggc gcgtcggggg
    11161 cgccttcgta gatcacgacg gtggcgccgt ttatcagcgg accgtagacg atatacgagt
    11221 gacccgtgac ccagccaacg tcggcggtgc aaaaataggt gtcctcgtcg cgcagatcga
    11281 acacatattt ggtggtaaac gccacctggg ttgcatagcc gcccgtcgag tgaaccacgc
    11341 ctttgggctt gccggtcgtc cccgaggtat agaggataaa cagtgggtgt tccgacggca
    11401 caatcgccgg ctcgcacacg cccgcttccg accgcaccag atccgtccaa aagtggtcac
    11461 ggcccggatg catcgagatg tcctgcccgg tgcggctcac gacgacgacg tgcttgacgc
    11521 tgggacaatg cacgagcgct tcgtcgacgt tgtgtttgag cggaaccact ttgccgcgtc
    11581 gatagccacc gtctgccgtc accaccgcga cggcaccagc gtcgttaatg cggtcgcgaa
    11641 tcgcttctgc ggaaaaacca ccaaaaatca ccgagtgggt cgcaccaatc cgcgcgcacg
    11701 ccaacaccgc aatcacaagc tccggcacca tcggcatgta gacgatgacg cggtcgccca
    11761 tccggatccc cagcttgcgc agcgccgtcg ccgctcgaca gacttcgcga tgcagttcgt
    11821 agtacgtcag cacacgctgg tcgccgggtt cgccttccca gatgagcgcc gctttgttgc
    11881 gtctgtgcgt cgacaagtgg cgatccagac agctctcggt gatgttgagc tgtccatcag
    11941 caaaccagcg cacaaatgga tggtctttgt cgtcgagccc ctgggttggc ggcgtgcgcc
    12001 aggtgatgtg cgccgcttgt tccagccaaa aaccatcggg gttctgctgc gctttttccc
    12061 gcaaaaggcg cagcccctcc gcatccaagt gagcctgctc aacaaacgtt cccgacggtg
    12121 gaaagcttcg cttttcagtg aggacggagt cgatggtctt catggaatca atctacctgc
    12181 aagcccacag accggcgaca gaaacgtttt gctatcctgg tttgcatgtt gggcgtcgaa
    12241 acactgcttc tgcccgtcag ctttctcgac tggctgcgga cgcggctgtt ttcgctgctc
    12301 ggtccgcttg ggcaaccgat tgtcagacgg ccagaactcc gggtcgcgct gcgaggcagt
    12361 gtcgcggtgc ttttggcgct cacagggacg atccttttgc cgctgtggat cttggcgctt
    12421 gggccgctca tcctcggggt gccgcatctt ttgtccgatg cgcgctattt ggttgtccga
    12481 cctggacatc agcgctcgcc gcgtcttgtg ctggctgttg gcattccgct tgtcatctgc
    12541 ggtctgggcg tcggcatgcc ggcgggactg gcggcgcttg cgggtgtgtt tttggtcagc
    12601 gatggcgcgc tgtggcgacg atgcatcggt ctttcgctgg tgggcgcgct cgcgctttgg
    12661 ggctacgcag cgcacagctt ttacccgcac gagctgtggc ttgcccatct gcacaatccg
    12721 attggcgtcc tgatttggtt tttgtggcga ccacggcacg agcggctgca cctgataccg
    12781 ctcttgctgt atgtggcgtg tgcgacggtg attggccttg gggttgtgca cgctttgccg
    12841 tttggtcttt cggcagcacc gcacagcgac ggttcggcgc tcgaattttt ccgcgacacc
    12901 ctggccccag agttttccga gacgtggggg acgcggcttg tgctcctata ctgctacgcg
    12961 cagatggtgc actactcggt gtggcttcag cttgtgccgg aagaagaccg gccaagacct
    13021 acgccgcgca gcttccgcga aagcctgcgg gtgctttttg cggacctgtc atggccgctt
    13081 cttctgctgg cgatgctgat tgcgctgtcg cttttgctgt ttgcactgcg cgatgtctgg
    13141 gcggcgcgaa cggcgtattt ccgcctggcg acgtttcacg gctatctgga gctgtgcgcg
    13201 ctcggactgt attttgtcaa aggacaaagg ccgcaaaaag cgcggcggct cgtcgaaact
    13261 gcgcagggat ttttgccggt gagtgctgct gcggcacatc gctagttcct acttcccggc
    13321 tcttgttcgg cgtatgctca ccgcaaaagg aggccgacat gaagtggaca ttcgcagcca
    13381 tggcgctcgg cggtgtactt ttggcgggtt tttcggcgca tgccgagggt gatgccgagg
    13441 cggcgcccgt aagccccggt caggttcggc atgttcctgc aacggcggaa accgccgaag
    13501 aggccagtcc caagacggcg ccacgcccac caacggttcg cagcgcagcg gccaaacccg
    13561 atggtacagc ggcgaccgtg aagccgatgc tgcccgccga ctatcggcca cacggggaaa
    13621 caggagccgc cgccaaagag accggagcca aagacgcggc caccaaaccc acgtccaagc
    13681 ccgcggctgg cgacaaagcg aaagatacga cggcgaaaaa acgcattccg ctcgcaccga
    13741 ccgaagccga gctaaaacgc gaaatcgcct tgcgcaacgg cggacccgat gcaccggcaa
    13801 cagacaaccg cccgacgagc gaaaagctcg cggacaaagc caccagcatt cccaaccagc
    13861 gacgcgagtg gccgacgacc gtgaacaata ccgccggccc aacatccaaa gccggtcccg
    13921 gcagcgcgca ggaccgcaaa ggcaccatga tcgaggccac cggcccgcag caaggtgtca
    13981 tccgcaatcg ctggtaaaat ccatccacca tgtcgtccaa gccgctgcgc tttgtcacgc
    14041 tcccgatcag ccactattgc gaaaaagtcc gctgggcgct ggaacgccaa ggcgtcccct
    14101 tcatcgagga aggccacggc ccggtgctgc acgctttttc gaccatgccg ctcacggggg
    14161 ggaaatcgcg cacggtgccg atcctcgtcg acgaaaatct gtcgccgcgc cgggttctga
    14221 tcgattccac cgactgtctg cgccacttgg ccgatcacta cggtgcaagc tggctgtatg
    14281 cccatcccga agccgcggcc ctcgaagacg agctggatga caagctgggt ccggccaccc
    14341 gtcgttttgc ctatttttac cttttgccag agcgtgcgac cgcggacgtg ctgacgcaaa
    14401 atgtgccggg ctgggaagca tcctttgcgc gggccatgtt cccgctgatt cggcgcggaa
    14461 tggccaaagc gatgaacatc tcgcccgaag gggcgcagcg ctcgcttggg cagctacgcg
    14521 agctgatgga acgatatggc gcgcggctgg ccgacggacg gcgctatctg tgtggggaca
    14581 cgctgacggc ggcggatctg acgtttgcgg cgctggcctc gcccgtgatt tttccgtcgg
    14641 ggtacgccaa gatcacgatg ccggacatca cggccatgcc gacgccactg cgggaagaag
    14701 tccagcggca acggcagtcc cccgctggtc agtttgtgct gcggctctac gccgaggagc
    14761 gcgctaaaac gatccgctca gcgtaagacc cgtctgggtg ctcgtccgca ctggggcaag
    14821 ctgaagcccg acgagcggcg ccggcagccg acggttgctg acgatgccaa ccaccatcat
    14881 cgcgacaccg cccatttgag ccactccgtc gacgagcgac cagttgatcg cccagattgg
    14941 atcgcgccaa atcaagctgc tgaccagcgg accgaccacc gggatcatca gggttcccga
    15001 cgcagagcgc tcggtgctga catcccgggc aaacgagctg ccttggctcg actgcaaata
    15061 caccgatccg gcgatcactg cggccagata gcccgaagca aaaagcgtcg cgccaccggc
    15121 aatcaagctg cggttgcgct gtcgaatcgc cacggtttcc gccgtccgcc gatgttccag
    15181 cagcgcggac agaagctgct gcatttccat gtgcacgtcg gcacacatcg aagaacccgg
    15241 cagcacatcc gctggcatga cgggcggcgg cgaaacaggc tgcatcggct gcgtcacaac
    15301 ggatgctggc cgcggaaccg gctgtcctga cggtggcggc agcggtggcg gcggcacaaa
    15361 cggcttgccg tttgcatctt ggagcacctg cgcctgtgcc gtcggtgtcg ccagcacgca
    15421 cagcgcagcc accgccagtc ccgacgccga cgccccgact cgagacgaaa aagcgcgcag
    15481 cagcgaacgg ttttgcatca tacgctgcac cataccacgc acgtcgacgc ggcgctcttt
    15541 cggttcacac cgtcgcgcaa agcccggaaa gcgacaaacg agcttcggct agaacggctt
    15601 gccgccgatt tggaagaacg tcgaccagcc cggtgccggc gtcggtgaaa cttccagcgt
    15661 cggcgccaag tcatagaacg ggtcggggta gtagtacgtc tggttgagga cgttgtagaa
    15721 attgaggctg gcccacaggc ggtttttgaa aaagtgcgca cgagcaccca ggttcaaaat
    15781 cgcctgcggc ggcagcttgt cgaacgtcag gtcagaaaag ttcgcgatcg tgcttccatc
    15841 ggcaagcgac agcgtgcgca gccggttcgg atcgtcgtag ctgccgacga ccatcagcgt
    15901 accgttggtt tccagccagc ccggaatcac cgacagcacc cagcccagat tgaacgagtg
    15961 ctgcggcatg ctgcgcagga cgccgcggtc gttcgtcgtg atgtgcaaaa acgtatagtt
    16021 tgccgacagc gtgtggtcgc cgatgtaaag acgcgccgct gattcgaccg agtgaatctg
    16081 ccgatgcgag ttgaagttgc gcgagtcttg tcccggctgg tcggccaacg ccccgtttgc
    16141 atcggtacgc cggttggtat agctaccgtt cgatatgacg ataagtccgt tcagatcggt
    16201 gtaggcgtag tcggcgcgaa gctgaagcgc gcgaatcggg ccaatgttgc gcaaaagctt
    16261 ggcgttccat tcggcctgaa tcgcttggct cttttcgttg ctcaggaagt ggctgccacc
    16321 aaactgcacg gcggctccgt ttgagtcggt gttgttgaac accggcggac ggaagcccga
    16381 ggcgtagttt gccttgacgt gcatgtctcg atagaagttc cacacgagcg acgcgctgcc
    16441 gaggaccgtt ccactgttgc cgaggttgcc gccgttgtac tgcgctttgc caaagccgcc
    16501 ttgcagacga acaccggcat cgagcgtcag gctgctaacc ggtcgaaacg tgtctgacac
    16561 atagagcgct gtgaccaagc ggtttgtgcc aaacaaaaac ggcaacttac agccggcttt
    16621 gaacaccggc atcgctgtgc ccgacttttc cagcggacag ccaatcggca gatcctgcgg
    16681 attcgactgc gggaagttga catacagttc gggcacccat tcgtagaaca agtcgccgcc
    16741 ccacagaagc cgattgcgca gcggcagcga ggcatcccca tcgatcgtaa gaccgacgcg
    16801 atacgaacga atctgggtgt caaatgtcgt gccgtttgga ttgcgcgccg agcccggcaa
    16861 gatgatcgcg tttaagtcgc gcacaaactc gatcgcgtag ctttttacgt cgagcccgag
    16921 ccgatcttta agaaaccgac ctttgtaaga cagcgtcacg aagcgatcaa acgactggag
    16981 atagttgcgc accggctttc cagcaggcgc gttgctgccc gtatcccagg caccgctcgt
    17041 cgggttgttg gcgaacgaca gcgacgtatt catcagcgca aacggcagcg aatacgacag
    17101 cgtgaccggc ccatacgaaa tcttgccgtc gacgttcaca atccacgacc gctcgggatt
    17161 ggtgtctagg ttggtcgaat atagatgcgg gccggatggc gagggcgcag cgttgcggta
    17221 cagcgacgcc agcacattga agcgcgaggc gacataggtt tcgtagctga cgtgctggac
    17281 gatgctcagc ttgggaccac ttttgaagcg gaagcttttg ccaaacagca cgtaggcgcg
    17341 aaagtcctgc ggcgagccgg gcccgtcgcc ataaccagcg ctcgcttcca gaccatttat
    17401 gtcgtcagcg tccttgctga tcaggttgac gatgccaaga aacgagtttg cgccccagag
    17461 cacaccgccc ggtcccgtca cgacttcgac gctcttgacc gtctctagcg gcaggccgcg
    17521 gttgtaggtt ccgacgttga gcaccggctc gaacatcgaa acgccgtcgc gcaaaagcag
    17581 cgccgcttgg gcggtcccgc gcaccagcgg aatcgacaac aagctgccgc cgttgctcga
    17641 tgacagccag cccggaatcg tttccagaac ttgggtgatg ttgcgatagc cacgggcttg
    17701 gatttcgtcg gcgcgcacga cggtgacgat cgatggcgct tcttgcaccg tcgtgacctg
    17761 cttggccgct gaaagcacgc ggtcggcgat gtcgatgtcg gtgttgccac tgccgcgatc
    17821 gacgtcggtt ttcgtcgcgt tgtcgacgac cggctcggga tcgggcaaga gcacgtcata
    17881 cgccggatca agcgccgatg ccgaccgcct ggctggtggc agcacttctt gggcgagcac
    17941 ccacgatggc aaacccaaaa tcagcgcact cacgaggaga ggttttttca cagcagacct
    18001 cccgcccgcg gcagcatgga atacgccatc ccgaccatca cccgtcccgt cggattgcgc
    18061 ggatcagacc ccaaaagtcc accgcctccg gtgagggtga gccgcagttt ttccgtcggc
    18121 aaaatataaa ttgaaccgag cgccgtgccg gtcatgttcg tcttgtcgcc cgtcaccgga
    18181 atcgtcccaa ccgcttcgat cccgagccca atcacgcgat gcagtcgcac cgtcagcgcg
    18241 ccgccccagg tgagctcatg tccaacggtc agtcgcagcg gcggatcgga cgcctgggcg
    18301 gtagttggat tgggttcaaa aagctggctg ctctgccgca cgactgcgcc aaaactgccc
    18361 agcacatgca gtcgaccaaa cagcccaccg agcagcagct tgggtcgaaa cgtgaagttt
    18421 ttgtcgccca gaaacgagtt gctatcgcca agcggcgccg tcacctcgag cagcgtgcca
    18481 atttcgaagt atttgccgcc gtaaaagcgc gctttcaccg caaaacgcgg atcgcgcacc
    18541 ccgacggtgc taagcacaac attttggcgt ggctgccccg catagatgcc cgtgcttacc
    18601 gtcgatcccg tcgttgcagg tggcgtcgga agaactgaaa ccgtcggatc accgacggca
    18661 ttggcatcgt agtaattgac cccaacgggc accattgcgg aaagcgacag gaaatcccac
    18721 agcccgaaat agaagccgac gtcgagcgtg acctgatttt cgatcaccgc aaacagcgct
    18781 tcttgccgcc ccatgttggt gtcgttgagc gtgagccgca gcggcttgcc cgcccaaccc
    18841 acgttcgcgc tcatgccaaa ctcgaactgc tgcggcgtct gcgcgcgatc caccacctgg
    18901 agcccatagg cgtccatcgc cggcggcgtc atcacgacgt cgatgctgcg atcggcccgc
    18961 gcgctcgaag ccaccagact gaccgcgcag gtgaaaaatg gcagaacaag cgcccgacgt
    19021 atcattgcaa cctcgcgtcg agctgggtct ccaccgaagc cgagccgacc agtgcggaaa
    19081 agttcgtcgg aagaccagga ccattggtgt tcgcgtacat cgcaaacttg atcgcgcccc
    19141 gtcccgctgc ggaatcaggc agccgtcgac cctggatcac cggatagtag atggcgtttg
    19201 gtaacttgtc gttgtccggc tcgatgcgga tctggatctc gatcagatcc ttcatgttgg
    19261 tcggcaccga aaaggtgatc cccgaaaagt tctgcgtcgg aagatcaggt gccgccgtcc
    19321 ccgcgccggt ctgatacagc agcatgccaa acaaaacgcc cgacagcggc ttggtgtact
    19381 gcttgggatt gcccacgtaa aagttgggat ggccgccgtc gcgataggct ttgcagacgt
    19441 tcagtcgccg gtctgattcg gcgtcgtctt ccgctgtcgt tggtgtaatc gccataagcg
    19501 ccccgctgcg acgcggttca ttggcattgt ccgggttgtg ctgaaccagc accaacatcg
    19561 tcgtcgccgc gcgccctttg gtcgccagat tcaggtccgc attcgtcgat gggaaaggcg
    19621 ttagcttgga caccaccttg cccacttgca aaagcagctg atcccgccca gccagcgtta
    19681 catcggacag cgcttcgtca tcggtggctc gctgcgcttt ttgggtgtag tactgcgcgc
    19741 cgcacgtcga tgaggacagc gaaccgcgcc cgcagccacc gtcgtcacga tccagcccgc
    19801 gaatggcgca tggctcactg ggatcgacga ccggcagaac ctgaaagcct gaaaatgcct
    19861 gcgggtcggt ctgtccaccc acgttggcga tgatccgctc gggcttggga ttgcccgcat
    19921 aataggcgta catctcgacg tggtgagacg ggaacggcag cgtcggcacc acgggcggcg
    19981 gcgtacaacc ggcaggaacc cccgtggcaa cacacggctg gggaggcagc ggccaggcaa
    20041 tcgacatcgc gaccaccgag cctgaaaacg tgtcgcagct acccgccaaa gccggcaaga
    20101 gtgcgaacaa aaaggacagc ttccgcgacg aggctaacac ataagaagcg tatcgaggcc
    20161 ccttcgggcc tgtcaatcaa ttcatcttaa tcgttgtctt cttcgtcgcc ttcgtcgccc
    20221 gcaccgtcag cttcgccgcc ttgcaccgcg aacagcgccc actcatgctt gccgcccgcg
    20281 agcttggttc cgcaatactg gcacgttacg gcgtcagacc cagccagcgc gccgccacac
    20341 tctggacaat cgagcgagga catcccgcgc cgactcttgg ccgtggccga ccgaccaagc
    20401 gccagcacat gcgtaaacgt catcggctca cgtccgtcga ccgaagcgct ccacatgatc
    20461 tcgacgtaag cgtagtcctg ttcaccgccg gtgagaaccg attcgagctc ggcgctaccg
    20521 accgcgacct gcgacagctt ggacggctca agcgacagct ccgagcgatc cgtttccgtc
    20581 gtcggcggct tcacacagaa gcgattgagc ttgtcgagct tgccggtcgc tttcgcttcg
    20641 atccacttcc agaacatcac cgatgcccgg tcttcgattt cctgtcggct cacctgggga
    20701 tcgcgctcgc gcagctcatc aagtcccgaa atatccccga cagccggctc agcccgccac
    20761 tcttccggct gcgtgatttc cgccagcacc cagtcgtgtt cccccgagtt gaccagcgct
    20821 ttgcagtact cgcacttgac cacttcgccc agcggcagat cggcaccaca gcttggacac
    20881 ttgccttcga gcgccgggat tccctttttg ctgtgtttgc cgcgacgacg aacaaacgac
    20941 caaacctcgt gatactgccc tagctcggct tttttggttt ggcttagcgc ttcgctcggc
    21001 gacagcgaca cagacacatc gcgatcgcgc gcctcgccga cgatcttgac gtgcaccgta
    21061 tcccagtttg gatcggccac cgccgccatg atgtccgccg acaccacccg ccaatcggcc
    21121 atcatgttgc gcagcccttg cgacttcaaa agctcgagct gcgtcgaaaa acgaacatac
    21181 acgccatccg aaatcacgcg ccgcgccgga cccatgttgc ccgaaagcca cgcctcgttg
    21241 accttgccga cgacggtctt ggtgcgcgca aggaacgaca tcggttcaaa ctctggatcg
    21301 tgctgccgca agtcggccag gcccgccttg gcgagtgatt cgtcgacatc gagcttcgct
    21361 cgctcgggct ttttgttccg tccagccagc cagatcagca agatgatcac caaaatgatc
    21421 accaccacca cgacccagcc gctgcccgaa gaggagctcg atcccgtgta aacaaccgtc
    21481 gtcgagccgc tcgatgaacc tgatgacgag ccgtaggacg acgaaccgga agaggacgac
    21541 gaaccgtagc tgcgcgagct gcccgacgag ctgctgccgc tggaatacga ccgactcgag
    21601 ctgctgccgg aagagtaact gcgcgagcta ctcgatgagc tgcgcgacga cgatgacgat
    21661 cgatagctct gtccgccgcc cggtcgagcc gctgcccaat cgaaagcgaa ccagaacacc
    21721 aaaagcacgg acaagagcca gaccacaccg cgtcgcagca taagcagcat cctagcgctt
    21781 tcgtcaggag ccgtcacggc cccaggctga cagcgaccgc acccaggttg tttggcgaaa
    21841 gaaaggaagc gacagaaacg gactggaggg gaactactcg gacagcttga acgagctgcc
    21901 gttttggacg atgccttcgc gctggcagac gtagatcagg tactgacggg cagcattgtc
    21961 gagcttgcgg tacgcatcgc cgaccagctt gagatccttg ttgcggcagg cggcggcacc
    22021 aatgatgcgc caagcgcggt tggtgctgac cttggccacc gatcgagcca ggctgatcgc
    22081 tttgtcgtag ttgccgttaa caaactcaga ctgcgcatcg gtgagcacct gctcggcatc
    22141 gccaccagcg gcgggcgctt cgaccttttc aggcttttcg gccttttcag gcttttcggt
    22201 cttttccggc ttctcgggct tctcgttgtc ggcgaccttg cccttctctt tgtccttgcg
    22261 gtgatgctct ttggtgccag cgttgccacc atcgttgctc accgacgagc taccgcccga
    22321 accaccacac ggctgatcct tggccgccag ggctttgacc tgttttggat cgacatcgag
    22381 caccatctgc acctgcgacc gcgcttcccc acagttgcct gctgcacgcg catccgatgc
    22441 gtttttgaga tggctctcga cgaacagcgg gaagattttg tcgtagtctt ccttcgcgct
    22501 cttttgatag accgaatctt cgggaatttc gcgatacgtc ttgagcgcag cgtcgtagtt
    22561 tccgctgcct gctgcacccg caaagcgatc gtagatctgc ttgttcttcg attctttttc
    22621 ggcaaccgtc cgttttgccg tcgcggcatc gcgggtgcga atcgcaatcg cattgttgcc
    22681 aatgatcttg cccgtcttgt cgagcacgtc ctgccagcgc ttcgccgcca gatcgctttc
    22741 aacatcgatc agggtcttgg cagcctcggc gtcgacatcg accttcggcg gctgggtcaa
    22801 gaaccagtac gcaccaccac cgacggcagc cagaagcaca agcgtcagga tgatcggcac
    22861 caccggcgtt ccccgctggt cgcgatcgac gatcattgcg tcgatctgtt ccttcgggat
    22921 gtcctgtccc ggcgggatga acaccagacg aacgtggccg agatcgactt tgtcgccttt
    22981 gcgcagctcg actttgccgt attcctcgcc attgacacgc acgccgttgg cgctctgcat
    23041 gtccatgatc tggaacttgt caccttcgcg aacgatcttg gcgtgatgac gagagatcga
    23101 gcggtgattc agcaccacgt cgttgtcgtc ggtacgaccg atcactgccg tggctttttc
    23161 gagctggaat tcctgacgtg caaagtttgt cgaaacaacg atcagccgtg ccggcttttc
    23221 gggaaccttg acgaccgggg cttgaaccgg ctgcgccaag gtcgctgcgc caggggctgg
    23281 aatcgccggc gtccccgaca tcagcgtcgg ttcggcgcga ttcactggcg tcgtctgcgg
    23341 gccttgaccg ggtacttgga ccttggccgc gcctttgtcg agcttgaccg agacctgata
    23401 gtcgccgatt tgaactcggt caccgtcgcc aacagcaacc ttcccttgga tgcggtcgcc
    23461 gttgaccttg atgccgttat agctcagcag gtcttcgatg aagagcccat taccctggcg
    23521 ggtgatccgc gcatgacgcc gtgagacgtt gcgctccgtc agacgaatcg tattcccttc
    23581 cttccgtc
//
LOCUS       DAORGN010000061        23352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_863_length_23352_cov_5.906297, whole genome shotgun sequence.
ACCESSION   DAORGN010000061 DAORGN010000000
VERSION     DAORGN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_23352_cov_5.906297"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            604..2127
                     /locus_tag="PKL17_08915"
     CDS             604..2127
                     /locus_tag="PKL17_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44889.1"
                     /translation="MIGQMVDRYRVVSQLGEGGMGAVYEAIDDSNSQHVAIKVLHKEH
                     ASNQQIALRFINEARAINVVDHPGIVQVMSIGQLPDQTAYIVMELLKGETLSARMKKS
                     PGSRLPVLDCVRLGKQIASALAAAHAKTIVHRDLKPDNIMIVPDPKVPGAERTKLLDF
                     GIAKVKQEYQANDQLQTRAGVMMGTPLYMSPEQCKNAGDVDDKADVYSLGVMLYRMLS
                     GKPPFSAAGTGELMAMHIYMQPQPLKEHDAAIPEPLIELVHKMLAKQRQDRPSMAQVE
                     AELEKVNIKVSAMMPAVRLPPPPATQPSTPSPPMAAEAKTVLSDGGAYAIPPPSGRDA
                     ATIMPTPSPVASGGGRGLRIVIVALVTLLALGIGAGVVGLLQKRDSVRLDPTDPKSLG
                     KTVDKLVNPADNPDSKKPKAAKIISWHVESDPSSADVYDVRKDELLGKTPFRVEQPIQ
                     PGEVELELRLEGYQHKRITVKADENQEPPVVKLEPVKVEKDPKPKKDRSKSRRHGKK"
     gene            2173..3687
                     /locus_tag="PKL17_08920"
     CDS             2173..3687
                     /locus_tag="PKL17_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNK44890.1"
                     /translation="MSQPGTQSTSTSSDEVVEKSVRGAATTTLAGLVGRAAGLVTTLL
                     VTHFVHKTEYGQANLALIIATVVNVLTLLAPQQALLTRRTLFAESSQIIQRFVVGSGV
                     LVFGLLFGLGRPLLGLLHESGTLPLLQVYCAALFFERLALIPAVRLRYELRFSHLVQV
                     DLLGDTVYVIVTMATAAYGAGAICLPLGALLRQVARVLWLKFGLRMSLLWSDVQLPNA
                     QTQTLRSELWQMMWPIYLASMVELSTLYMDNLFVGRVYSTGAQGIYAVGYTVMMTPCE
                     TIAMYATSALVRALGLDDRDRRQQNFLAGLRYVTLLLLPIATGAALVGPTFESALLPP
                     RWHGIAQIMAGLSLGAVSLGFHRMAFAQLTALHKSRLAALVYATQISCFVLGLWLVAR
                     FDPQRLHMERTAYAVSGALLLAGVTGVLATVVTERLRLSWLAYALGPALLGSSAMAVA
                     LAGLQWLLGRFAVPQTAPRLLVEVLLGMVVYGSYLRLCHPSLFDEVRRFIARRN"
     gene            3703..4239
                     /gene="tsaE"
                     /locus_tag="PKL17_08925"
     CDS             3703..4239
                     /gene="tsaE"
                     /locus_tag="PKL17_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNK44891.1"
                     /translation="MSHAAILAARSQPVSPDLRLVSRGPTGTRRIGQQMGALLSAGDV
                     LLLEGPLGAGKTCLTQGLAIGLGVDAATAVQSPSFTLVNQYSGRLPLYHADLYRLSHP
                     QELFELGLWEAAETGGVVVIEWASRFPGEMPTDHLSVELLPGQGLYRQVIVRATGSRS
                     AQRLAEIATKLAPRRRRS"
     gene            4236..5255
                     /locus_tag="PKL17_08930"
     CDS             4236..5255
                     /locus_tag="PKL17_08930"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNK44892.1"
                     /translation="MSETMIWNINIDTLRGGYLESLSRVRAVVVDASGRVIMQAGNAE
                     EPVFWRSTAKFVQALSLFASGAVDRFGFGDRELALACGSHSGGDEHVALAQRMLDLIS
                     ASASDLHCGPHVPLGPEEAKRLAAEKKTPTKLHNNCSGKHSAMLASCRARGWSLVDYN
                     RHHHPLQQEILAHLSAVSGVDAQHIQQAVDGCGAVVFRTPLIGLARAYARLAGDALPA
                     PHREAGKRLRTAILAAPDLVAGPGRLCTDLMEVSSKNLIAKVGADGVYGLGALHTPDG
                     PCGLAVRIEDGNMRLLSPLVCKLAAALGWLDSAQMEKLQAHWHMPLINHQKELVGDIH
                     VRIED"
     gene            complement(5252..6202)
                     /locus_tag="PKL17_08935"
     CDS             complement(5252..6202)
                     /locus_tag="PKL17_08935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNK44893.1"
                     /translation="MIDLSFSTSLAESLWATASSGQAVTTSASGSPLWSLPLTVLVPL
                     LGLPAFWIVSFLIYCVKCAIFGMDRTPRIDQVVKTPWLPRIVMEFGYWMFRIPVRICI
                     ALGITPNMITFGSMLLTVLAAVAVAIGHFAFGGWVLLFAFTCDAWDGIVARATNNCTA
                     SGEFFDSTMDRYNDLFTFLGYLYYYRNQPWVFVVTALAMVGSTVVSYSRAKGDAVGIN
                     ANVGYMQRHERAVWLGVSTALSPIAAAYIEPDAAVPLYHVAAGVLVLMAVMTNITAIW
                     RIRVVMRGLSGRAAGSKPARVSKETAAPAPADLTPQPTHS"
     gene            6618..7322
                     /locus_tag="PKL17_08940"
     CDS             6618..7322
                     /locus_tag="PKL17_08940"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNK44894.1"
                     /translation="MRSSVEKGGSGEPAPSSVPAVASAKPKNTTDVPLRDASSATAPN
                     LVFDGKGYLMVGRGQLAVSGIPPYTASLYLEHVGARAQFGSLTTRAPTRAQLMSENRA
                     QYFVIWGRFAKLMVLKFAKPATKAELTEMFRSGMPEVFSDRAPVELHKDADSFLRLID
                     RDVAEGQELRLHVSEQSQIDVYFDGQKQAGPQNAKLARHVIEIWLGYHSAARSLRQVL
                     IDKIEVLKTPVPRPPK"
     gene            7491..9599
                     /locus_tag="PKL17_08945"
     CDS             7491..9599
                     /locus_tag="PKL17_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44895.1"
                     /translation="MSRLLHNLFSVARRRLSIAMLLGAAASGLSSCDTTTKQAVGCNT
                     SSDCPGGQICGSTGQCLPKPKSLVLTLTKLGEGIGSVKSTPAGIDCGTTCSASFDVGT
                     PITLTATAQSGSLVSGFSIGCSSTDATCTFTPTTEDPVQVGVNFALAPPSQPPPLCNS
                     YGFCWENPRPQGNKLNDVAVLPSGEVWAVGDAGTVVRRVGTTNTLVSSGTTQALYGAA
                     VVGTDVVIVGQGGTAMRGAGSAVTTESSGVVQDLYDVGATSAGAVAVGAGGKILRRSG
                     IAWTGDTSPTTQALRGVSVTGTDIYAVGDAGTVLANSGAAWRVVSDAQFGSKALSSVA
                     GLGGAAYIGSVTGEIFRQGSPWSRVCCNNLPDITGMTSSSFGLLAVGFTVGGSILQSS
                     DGTTWNTAVDGASTLLYGVAATTGEAWAVGDAGTMRRSTDGLSWPTISTGATGLIRAV
                     AGTTAGYYAAGQSGVLLRSVGTAVLSYTAAGTPDFFGVSAASPTEAWAVGDVGTIYQF
                     NGTAWQKVVSGTGSALRAVWAAGPGDAWAVGDAGTVVHVKNGSASSMSGGTTQNLLSV
                     WGVSATDIWAVGAAGTVVRYQGSSFAPVTAPATTRAISSVWGNLASNVWMVAGPDVFV
                     WNGNNYQKYTPSLSDMTAVGGFSTEVYVTGSKGQLYRYAGPGFTSVETGTRNNLYGIA
                     LSATSMWLVGDNGSVLSKLR"
     gene            complement(9600..9830)
                     /locus_tag="PKL17_08950"
     CDS             complement(9600..9830)
                     /locus_tag="PKL17_08950"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HNK44896.1"
                     /translation="MPSRYFRVPLAEIAYVRMIVEAFDGLAVVRSLCDSRGEIEWLIG
                     EGLEAEADEVARRLQVLTGMCPIERPADWQDL"
     gene            complement(9846..11996)
                     /locus_tag="PKL17_08955"
     CDS             complement(9846..11996)
                     /locus_tag="PKL17_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44897.1"
                     /translation="MTCSRMLFLGVLVGGAALLIPTQPLYAQAAGGAAKAPAAKAAAP
                     APAAKTAAPAPAAKAAAPAPAVAAYPGGVPEAKLRSSHHQVEVVSSTDRHAARKDEVV
                     LPGTRVDSAAGSGAEVSFNDGTRVFVGENTSFSVYGVPPPAAAKGPAKKFTPGNNTIT
                     VGEITVVVPAAPAVLGAAAPAGKKPAAKPAPVKVPRTAMIVTPVGKVTVNPGSAARIS
                     VEPKTGATRVSLYDGAAALAGKGKTINLTPNQSSTIANLTTPPSVAAPLPAAPTITGV
                     PGLVLSTGGPVEVKGSYAAAGGAAPASWQVQVAQDAAFETMVKDGRVPATETRVMPSA
                     VGPGDYFVRVSAVSAEGTVGPWSAPAHVQVARVVLWPGGEGKRAAIQVDGKGVFCGLD
                     GGELQAVGQPMPLQPAREHNLRCATAQSGAKPEDSSEFKVSATQSGPVVAKLDVGAPA
                     FTPQDGSREVRLQLSDAAGSPITGANVTAEGTGGSRADAVRELPGTGTYVTTAHWQPG
                     KTGLGLRYTINGVENYESALPDAVPTTGKEEQKDEPASTDRPPTKSFAVELGLMPLAH
                     VDIPRGVFGVGGALEIGGRIKLPYGAFAFSLRPQYEYFAPAPGASHIVGGGVLLAYRF
                     RSLDAAWAPYIGVMPQFLAEYSFLARDGIQIQDGSWMSQVGLGGLLGAEIRLRRGSVF
                     VEAGYRHIIWGRAERADYASLNGVFGTMGYRVNF"
     gene            complement(12143..13267)
                     /gene="alr"
                     /locus_tag="PKL17_08960"
     CDS             complement(12143..13267)
                     /gene="alr"
                     /locus_tag="PKL17_08960"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNK44898.1"
                     /translation="MAEIDLGALRRNADVLRSAVGSAKTGLLAVLKADAYGHGAVACA
                     RALEKKVWGFAVSLVEEGVELRRAGIEQPIVVLGCYYGLSHRDVIAYRLTPVIADVRD
                     LSRFARAADELSAPRVGIHLKLDTGMSRLGMRDGQLDPFLSELGHHNGLYLSGLCTHL
                     YDADGADSAPTEEQLRRLSQTLSLLQSQGVRPGIVHVANTAGAVRFADARFDLVRAGI
                     GMFGVAPDHTQLATLWPVMTLRTKIIALRKLPAGETVSYGGRSRVNRPSICATLPVGY
                     ADGYTRRMSDRAFVLCGGRHCKVFAPITMDMTMVDVTDVPDVKVGDDVVLLGSQSGPD
                     GQSGRITLDEMASWADTIPWEICTVVSKRVPRIYTGDGRP"
     gene            13399..14250
                     /locus_tag="PKL17_08965"
     CDS             13399..14250
                     /locus_tag="PKL17_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNK44899.1"
                     /translation="MSRLSVSQIGAVLVALSLGSSPAWADAPWLGLVLQPGPQGGAQI
                     VDVYPDSALAPLVKGQKVQLGETVVGIDGKPVSKPKELSERVQKLKVGQIVSLKLQRA
                     GGPVHEVQATLTARPSEQLLEHQAMKMEEERQKRMIGHPAPEFVVELLHGPKLPAAAL
                     AGTVAALKGQPIVLVFFASWCGPCLREIPQLTAMQALRPDVRVLAVSTEETDKIRDVI
                     TRYAPQYSIGRDLERRAYGAFGVASYPTTFLIDGAGIVRAVDHGSLDTVQAALDKIPA
                     PNSAPAK"
     gene            complement(14255..14551)
                     /locus_tag="PKL17_08970"
     CDS             complement(14255..14551)
                     /locus_tag="PKL17_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44900.1"
                     /translation="MKRIALAAVIIGSFATGMVVSGCFTPVYSDCAFRCASEDPKCPA
                     EYECRSDGYCHLPDGTAICAIPRAIDLSSVSAVDLSSAADLADPGDAATTPDGM"
     gene            complement(14551..15261)
                     /locus_tag="PKL17_08975"
     CDS             complement(14551..15261)
                     /locus_tag="PKL17_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44901.1"
                     /translation="MVRGAYSGALILALLLVGPDVANAAGSHHRHHRRAHKESAQPAQ
                     AVSGKPAVQATPAAVGTAPVRAAEVEPPPTETEPPPLEVEPPPEALREDPIEPKPDPK
                     LQAEKQALARRQRIFSGLGFASAALTAGLLVTGTTLGVLAQNRSDDLSRLTVQSENGI
                     PPLYDADQRSTYERLQQEGLLFRSATIGFFVAGGVAAIGTGLLFYGQSRTESAQKKLS
                     LFPVVTGNTTGLALLGRF"
     gene            complement(15264..15983)
                     /locus_tag="PKL17_08980"
     CDS             complement(15264..15983)
                     /locus_tag="PKL17_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK44902.1"
                     /translation="MSQSESGKPLIVIRNLVKEYHLGKTIVPALQGVDLDVQRGEFTV
                     VMGPSGSGKSTLLNIIGCLDSPTSGTYEFDGQEVGNRDFDKLADLRNRKVGIIFQSFN
                     LIPVLNVSENVEFPCLMRKDPEELSSLRQRVVKLCEDVGLGPYMKHRPDELSGGQRQR
                     VAIARALITHPELVLADEPTANLDSKTSDQIISLMQRLNQQMGVTFIFSTHDPRVMQH
                     AKRVVYIADGKIVEHNPMTAH"
     gene            complement(15993..17528)
                     /locus_tag="PKL17_08985"
     CDS             complement(15993..17528)
                     /locus_tag="PKL17_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44903.1"
                     /translation="MSSLALQFGRKGVARRLRTGLLFGILCGLLCGLFCTSASAQESL
                     DDIDGTKKAETGKKISADVNGYVDNRFQYAYINPSKTLVPTINQPSLLEILEGNIQLK
                     VSIGQKAFVYADVSLIYQNGWLYYDRDQNGDRVKIDDHDVSTLRPFIVPSELYVSVAP
                     KNWLNLLVGKKRIIWGSGFAFNPTDLINPPKDPTDPNFQRAGQWVARIELPFEKFTIS
                     TLFAPQVLYQQNGLPFAMMKYPDYQLSDGTKPADDAYHYLFAARLYLLLFNTDINLFY
                     FFSNKYNDSFENTSRFGGSFSRYFFTDYELHFEALFQFGSGKLFANHACANGGICNPS
                     TALSASKLTDGNLYPRLLVGGRTQFRDESTLSLEYYYQGDGYSALEFEDYLRLLARAQ
                     AAGITTISQPTGGTTGAVPQRFTFDPLRRHYLFLSYNKPKIRDDFSIGATLIAGLSDL
                     SGTISPTVSWNAQEWLTLSLFGFIPIRGIPVGTVNINDVSYSEYSLLPMDFRFLFEAR
                     AFY"
     gene            complement(17532..18260)
                     /locus_tag="PKL17_08990"
     CDS             complement(17532..18260)
                     /locus_tag="PKL17_08990"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNK44904.1"
                     /translation="MLRTSFASALLLLSLSPSVAFADPTPEQLLKRYDEVMSPKTFDG
                     LLVMVAHRQDDTTRTYKFRALKSEDDKIRTWFYEPAAAKGQEMLRVEDNMWVYMPNLK
                     RALRLASRESFQGGDFNNGDVLRVNYVADYVAKLIPSGDDKLWLLDLTAKNKEAAYDH
                     IKLWMTKDKQQPAKAEYYAASGKMLRSAVFEDVKNFHGLERPSHITMRNELATKRFSE
                     MTTLDMKLNVDIPAQKFVLDDLGR"
     gene            complement(18272..19483)
                     /locus_tag="PKL17_08995"
     CDS             complement(18272..19483)
                     /locus_tag="PKL17_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNK44905.1"
                     /translation="MRLVLLALRNLTRNKRRTLLTALAIVFGAMAIVLLQGFVNGFIR
                     NNIEAIVMSKLGAVQVYRKGYLGSDDPLKKSMPHDPALMARIRKVPGVVEVAPRIAFD
                     GMVSNGAESTMFVATAIEPDLEYKVCPERPKRVAKGSTPLTQQQRGMTLIGKTLSDSL
                     KANPGSTLVMQAAGPHASTNALDIEVSGYLPTLNLIESKRMATVQVGFAQELLRMKGQ
                     VTEYVVGVHNLEQMNDVAQRLRTELGDDYQVTTWEDIDPATRTRVSSLGYVLIFIAVV
                     LFLLVATGIVNTMMMSVYERVREIGTMLAVGVRRWQVTALFLLEAMALGLTSSVFGSL
                     LGYVVVLWLGQRGIPMRPPGGDLLWTYPHVQPSFMLIVIGFSITGAVLSALYPAYKAS
                     RLSPTDALRAT"
     gene            complement(19485..20708)
                     /locus_tag="PKL17_09000"
     CDS             complement(19485..20708)
                     /locus_tag="PKL17_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNK44906.1"
                     /translation="MLPPLLRIALRNVFRNRRRSLITFSAVFLALGIMVSIRGFLNGL
                     QATLRESVVQGQTGALQVHKAGFLKSVNATLEMSIPSDAAFLAKIAAVPGVKSVTARI
                     MFGGMANANDISTAAMFTAIDPVHEVEVCPRRNDMIYRGKGLRGSGPTAAVLSPELAA
                     NLGVKIGQRATVLTGDRDGVMNALDFDVVGEYGQPGLPLPDKKLGFVPLPFAQELLRM
                     EGRATEIAIGIHHYDDAETLKPVLQAALGSEYEVSTWHDIASYVDDAIAMQNFILNLI
                     AGVFLFVALLGITNTMLMSVLERTREIGTMMSVGVRRRQILTLFLLEASVLGLLGGIS
                     GALVGGSFVAYYGHKGMVLHIPGSLLPLYVFPTLKLSYILSILAIAAGGAAIAALWPS
                     VRASRMRPVEALSAV"
     gene            20821..21255
                     /locus_tag="PKL17_09005"
     CDS             20821..21255
                     /locus_tag="PKL17_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNK44907.1"
                     /translation="MAEVPQITKDLAQKFFALLLRHRAYFQGLVQELGLSPPHVLVIQ
                     HLSDGALTMRELSVRTVCEPSHLTGLVDKLEERKLVIRKVDPNDRRSKLVSLTRTGRT
                     FRDKLMARMGAPVPWMFTLSEADQAQLLSIIERVLAQTDGKL"
     gene            complement(21267..22313)
                     /gene="hypE"
                     /locus_tag="PKL17_09010"
     CDS             complement(21267..22313)
                     /gene="hypE"
                     /locus_tag="PKL17_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNK44908.1"
                     /translation="MSSHRLRPLDLIDGRIELPHGSGGRATAQLVSELFARHFQSDAL
                     SAAHDAATLPVPGGHIAVSTDSYVVSPLFFPGGCIGDLAVNGSVNDVAMAGADPIALT
                     IGFILEEGFPLRDLDRICKSIASASAQVGIPIVSGDTKVVERGKADGVFISTTCLGII
                     PPAVHIAPTRATPTDAILVSGPIGNHGIAILSQREGIAFDCDVTSDTAPLHSLVSAMR
                     GVETDLHVLRDPTRGGVAAALHEIAETARVNMDLQASAIPIERPVRAACELLGLDPLH
                     LACEGRLLAICAAHAADRLLAVMRSHPQGRLAARIGSVTEQTDLPPIISLRTELGGRR
                     IVAWPAGEQLPRIC"
     gene            complement(22306..>23352)
                     /gene="hypD"
                     /locus_tag="PKL17_09015"
     CDS             complement(22306..>23352)
                     /gene="hypD"
                     /locus_tag="PKL17_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNK44909.1"
                     /translation="HALCQSGLLDRLPAAIQMIHGPGCPVCVLPTGRIQQAIDLVLRE
                     PVILCSYGDMLRVPALGHKSLLSARAQGADVRVVYSTEDALAVARQNPTRQVVLFAVG
                     FETTTPMTAAAMLTAKQEPIRNFSVLVNHVLTPPALTALLQTAQDQVRIDGFIGPGHV
                     ATVTGSDAFLPFAQRYRKPIAIAGFEPTDLLLALHAVIDNINAGTAHVENTFSRAVTQ
                     AGNLRAKQLIEQVLEVRESFAWRGLGDIAHSALRARQQVADFDAEKRFSLSTPQVPDH
                     KHCRCPDVLRGICAPTECTLFAAACTPDTPIGSCMVSSEGACAAYFQIGRTRRREVST
                     ARAHATSRPGCSDV"
BASE COUNT         4611 a         6754 c         7441 g         4546 t
ORIGIN      
        1 cctgcgccac gatcccacag tcgaaatcac cggcgacttc atccttccga agtaaaggac
       61 gttcggcacg ggcccgattc cttgcgcggc gtgaatggcc acgctcaatc ggttgcggaa
      121 gggctgccgc taccggatgt gcggaacaaa agtgtgcggg aagcgctgcc acgcaggttt
      181 tgtcgcgcaa aagtgtgcgg gaagctgggc tgccacgcac tttttgcgca aaaagctgcg
      241 ggaagctggg ctgccaacga cttttgtgcg caaaagagcg cgggaagctg ggctgccaac
      301 gacctttttg cgcaaaagcg tgcgggaagc tgggctgcca acgacctttg tgcgcaaaaa
      361 gctgcgggaa gctgggctgc catgcacgtt ttgcgcgcaa aaacgtgttg gcagcgctgc
      421 cggggcgcaa aaaacagaac cgatccgtgt gggaagcgct tccgcagcag gatggacggt
      481 ttacccgctc ctagtgcccc ctacgcggtt caagttggga tatactagct gggccgtgcc
      541 ctgttttttc agctcgcttt ctacctgtaa agacgtcgtg gccgtcccag tcaggaggcc
      601 ccgatgatcg gccagatggt cgaccgctac cgcgtggtct cgcagcttgg tgaaggcggt
      661 atgggcgccg tgtatgaggc gattgacgac tcaaacagtc agcatgtcgc catcaaagtg
      721 ctgcacaaag agcacgcctc gaaccagcag atcgccctgc gctttatcaa cgaagcgcgt
      781 gccatcaacg tcgtggacca tcctgggatc gtccaggtca tgtcgattgg gcagcttcca
      841 gatcagacgg cctacatcgt catggagctg ctcaagggcg aaacgctgtc ggcgcggatg
      901 aagaagtcgc cgggcagcag gctgccggtg ctcgactgtg tgcgactggg caaacagatc
      961 gcgtcagcac tggccgccgc ccacgccaag accatcgtcc accgggatct gaagccggac
     1021 aacatcatga tcgtgccgga ccccaaggtt ccaggggccg agcgcaccaa gctgctcgac
     1081 tttggtattg ccaaagtgaa gcaggaatat caggccaacg atcagctcca gacacgggcc
     1141 ggcgtgatga tggggacgcc gctctatatg tcgccggagc agtgcaaaaa tgccggcgat
     1201 gtggacgaca aggcggacgt ctattcgctg ggggtgatgc tgtaccgaat gctttcgggc
     1261 aagccgccct tttcggcggc gggcacgggc gagctgatgg cgatgcacat ctacatgcag
     1321 ccgcagccgc tcaaagagca tgacgcggcg attcccgagc cgctcattga gctggttcac
     1381 aagatgctgg ccaagcagcg ccaagaccgc ccgtcgatgg cccaggtcga agcagagctg
     1441 gaaaaggtca acatcaaggt gagcgcgatg atgccggcgg tcaggctgcc tccgccgccc
     1501 gcaacgcagc cgagcacccc aagtccaccg atggctgccg aagccaagac ggtgctatcg
     1561 gatggagggg cgtatgcgat tcctccgccg agtggacggg atgccgcgac gatcatgccg
     1621 acgccatcgc cggttgcgtc agggggcggt cgcggtctgc ggatcgtcat cgttgcgctt
     1681 gtgacgctgt tggcgctggg aattggcgct ggggttgtcg ggcttttgca gaagcgcgac
     1741 agcgtgaggc tcgatccgac ggacccgaag tcgctgggca agacggtcga taagctggtc
     1801 aacccggcag acaatccgga cagcaaaaag cccaaggccg ccaagatcat cagctggcat
     1861 gtggaaagcg atccctcgtc ggcggatgtc tatgacgtgc gcaaagatga gcttttgggc
     1921 aagacgccgt tccgggtgga acagccgatt cagccagggg aagtcgagct ggagctgcgg
     1981 cttgagggct accagcacaa gcggatcacc gtgaaggcgg acgaaaacca agagccgccg
     2041 gtggtcaagc tagagccggt caaggtcgaa aaagacccca agccgaagaa ggatcgcagc
     2101 aagagccggc gacacggaaa aaagtagccg acggttgcgc caagcggcga gacccgacga
     2161 tacttggccg gcatgagcca gccgggcacg caaagcacat cgacgtccag cgacgaagtc
     2221 gttgaaaaga gcgtgcgcgg cgcggcgacg accacgctgg cggggcttgt gggccgggcg
     2281 gcgggtcttg tgacgacgct gctcgtcacc cactttgtcc acaagaccga gtatggacaa
     2341 gccaacctgg cgctgatcat tgcgacggtg gtaaatgtcc tgacgctgct tgccccccag
     2401 caggcgctgc tgacccggcg aacgctgttt gcggagtcat cgcagatcat ccagcgcttt
     2461 gtcgtcgggt ccggtgtctt ggtgtttggc ctgctgtttg gtttgggcag gccgctgctt
     2521 gggcttttgc acgaatcggg gacgctgccg cttctgcagg tctactgcgc ggcgctgttt
     2581 tttgaacggc tggcgctgat tccggcggtg cggctccgat atgagctgcg cttttcccat
     2641 ctggtgcagg tcgatctgct cggggacacg gtgtatgtca tcgtgacgat ggccaccgcg
     2701 gcgtatggag ccggggcgat ctgcttgccg ctgggtgcgc tgttgcggca ggtggcgcgg
     2761 gtcttgtggc tcaagtttgg gcttcggatg tcgctgcttt ggtcagacgt gcagctgccc
     2821 aacgcgcaga cacagacgct gcgcagcgag ctgtggcaga tgatgtggcc gatttatctg
     2881 gcgtcgatgg tcgagctatc gacgctgtac atggacaacc tgtttgtcgg gcgagtgtat
     2941 tcgaccgggg cgcagggcat ctatgcggtt ggctacacgg tgatgatgac gccgtgtgaa
     3001 accattgcga tgtacgcgac aagtgcgctg gtcagggcgc ttggactgga tgatcgcgac
     3061 cgtcgacagc agaacttttt ggctggtctg cgctatgtga cgctgttgct tttgccgatt
     3121 gctaccgggg cggcgctggt cggtccgacg ttcgagtcgg cgctgctgcc gccgcgctgg
     3181 cacgggattg ctcagatcat ggcggggctg tcgctcgggg cggtgtcgct tggctttcat
     3241 cgcatggcgt ttgcgcagct gacggcgtta cacaagtcgc gactggccgc gctggtctat
     3301 gcgacgcaga tttcgtgctt tgtgctgggg ctttggctgg tggcacgttt tgacccgcag
     3361 cggctgcaca tggaaagaac cgcgtatgcc gtatcgggcg cgctgcttct ggccggcgtt
     3421 acgggcgtgc tggcgacggt ggtaacggaa cgactgaggc tttcgtggct tgcctacgcg
     3481 ctgggcccgg cgctgctcgg ttcatcggcg atggcggtgg cactggctgg tttgcagtgg
     3541 ctgcttgggc gctttgcggt tccacaaacc gcgccgcgcc tgctggtcga ggtgctgctg
     3601 ggcatggtgg tctatggatc gtacctgcgg ctttgccacc cgtcgctctt tgatgaagtc
     3661 agacgcttta ttgcccgacg gaactagggt aatactcagc ggatgtcaca tgcagcgatc
     3721 ctagccgcaa gaagtcagcc ggtttcaccg gacttgcggc ttgtgtcgcg cgggcccacg
     3781 gggacgcggc gcatcggtca gcagatgggc gcactgcttt cggcggggga tgtgctgctt
     3841 ttggaagggc cgctcggggc ggggaaaacc tgtctgaccc aagggctggc catcgggctc
     3901 ggtgtcgacg cagctacggc ggtccagagc ccgtcgttta cgctggtgaa tcagtattcg
     3961 ggacggcttc cgctctatca cgcggacctg tatcgcctgt cgcatccaca ggagcttttc
     4021 gagctgggcc tgtgggaagc cgcagagacg ggcggcgtgg tggtgattga gtgggcgagc
     4081 cgttttcctg gtgagatgcc gacggatcac ttgtccgtcg aactgttgcc cggacaaggc
     4141 ctttaccggc aagtcattgt gcgtgccact ggatcgcgtt ctgcgcagcg tctggcagag
     4201 attgcgacca agctggctcc acggcggagg agatcgtgag tgagacaatg atctggaaca
     4261 tcaacatcga caccctgcgc ggcggctatc tggagtccct gagtcgcgtt cgcgcagtcg
     4321 tcgtcgatgc cagtgggcgc gtcatcatgc aggccggcaa tgccgaagag cctgtgtttt
     4381 ggcgttcgac cgccaagttt gtgcaagcgc tgagcttgtt tgcgagcggt gccgtcgatc
     4441 gcttcggctt tggcgatcgg gaactggcgc tggcgtgtgg atcgcattcc ggcggtgacg
     4501 aacatgtggc gctggcgcag cggatgttgg atttgatctc ggcgtcggcg agcgatctgc
     4561 actgtgggcc gcatgtgccg ctgggaccag aggaagccaa gcgcctggcg gcggaaaaaa
     4621 agacgccgac caagctgcac aacaactgct cggggaagca ctcggcgatg ctggcatcct
     4681 gtcgggcccg cggctggtcg cttgtcgatt acaaccgcca tcaccatccg cttcagcagg
     4741 aaatcttggc gcatttgtcg gcggtaagcg gagtcgacgc gcagcacatc cagcaggccg
     4801 tagacggctg tggtgcggtg gtgtttcgga cgccgctgat tgggctggcg cgggcgtatg
     4861 cacgacttgc gggcgatgcg ctgccggctc ctcatcgcga ggctggcaag cgtctgcgca
     4921 cggccatttt ggcggcgccc gatctggtgg cgggtccagg gcggctgtgc acggatctga
     4981 tggaagtgtc gtcgaaaaac ttgattgcca aggtgggagc ggacggcgtg tacggcctgg
     5041 gcgcgctgca cacaccggac gggccgtgtg gtctggcggt cagaattgaa gacggcaaca
     5101 tgcggcttct gtcgccgctg gtctgcaagc tggcggcggc ccttggctgg ctcgattcgg
     5161 cgcagatgga gaagctacaa gcgcattggc acatgccgct tatcaaccac cagaaagaac
     5221 tggtgggtga catccacgtc cgaatcgaag actaagagtg ggttggttgg ggggttaggt
     5281 cagcaggggc gggggctgcg gtttctttgc tcacgcgggc gggcttgctg ccggcggcac
     5341 gaccggaaag acccctcatc acgacccgga tgcgccagat ggcggtgatg ttggtcatga
     5401 cggccatcag cacaagcacg ccggctgcga cgtgatacag gggcaccgca gcgtctggtt
     5461 cgatataggc cgcagcgatg ggcgacagcg cggtcgatac acccaaccag acggcgcgct
     5521 cgtggcgctg catatagccg acgttggcgt ttatgccgac cgcatcgccc ttggctcggc
     5581 tgtagcttac gacggtgctc cccaccatcg cgagcgcggt gacgacgaac acccacggct
     5641 ggttgcggta gtaatagaga tagccgagaa acgtgaacag gtcgttgtat cggtccatcg
     5701 tcgagtcaaa gaactcgccc gatgccgtgc agttgttggt tgccctggcg acgatgccat
     5761 cccatgcatc gcaggtgaag gcaaacagca gaacccagcc accaaaggca aagtgtccaa
     5821 tcgccaccgc gaccgccgcc aggacggtaa gcagcatgga accaaacgtg atcatgttcg
     5881 gggtgatgcc gagcgcgatg cagatgcgga cgggaatccg gaacatccag tagccaaact
     5941 ccatgacgat gcggggcagc catggtgtct tgacgacttg gtcgatgcgc ggcgtgcggt
     6001 ccatgccaaa gatggcgcac ttcacgcagt agatcagaaa cgaaacgatc cagaaggcgg
     6061 gcagaccgag cagcggaacg aggacggtca gggggaggct ccacagcgga ctgcccgaag
     6121 cgctagtggt gacagcttgc ccgctggacg cagtggccca gaggctttcg gcgagcgagg
     6181 ttgagaagct aaggtcgatc atgagaatcc ttgggtgcgg tcgtcgagtt ctccgctggg
     6241 cgacatcgca aacgtgcgat aaatggtaag aaatggtgca agacggctgc caacggactt
     6301 ccgctcgcgt tcttcggcca cccccgacgg cgacgtgcca gcggacactc tgtcaaaaag
     6361 ttaccacgtc tgcggcgggg cgcggcgtag gggcgcggat tgtcgaaaat tcgacgcttc
     6421 gctggcttcg cgctgtgggg attggtcttt tgcttgtgtg ccagggtttc ccggcgcatg
     6481 cggaagatgc cagcagcaag tcgagcagtg atgctgcaaa gaggtccgcg catcatcgca
     6541 agccgtatcc tccgctgcgc accgccagtg gtcagccggc tgcatcggct tcacattcaa
     6601 cggcaaacgt accgccgttg cgcagctcag tagaaaaggg cggctcgggg gaaccggcac
     6661 cttcttctgt cccggcggtg gcctctgcaa aacccaaaaa tacgacggat gtgccgctcc
     6721 gtgacgccag ttccgcgacg gccccaaacc tggtgtttga tggcaaggga tacctgatgg
     6781 tgggccgcgg acagctcgct gtgtcgggga ttccgccgta cacggcttcg ctgtatctgg
     6841 aacatgtcgg tgcgcgggcg cagtttggtt cgctgacgac gcgggccccg acccgggcac
     6901 agcttatgag cgaaaaccgc gcccagtatt ttgtgatttg gggacggttt gccaagctga
     6961 tggtgctcaa gtttgccaaa ccggcgacca aagccgagct gacggagatg ttccggtccg
     7021 gcatgccaga ggtcttttct gatcgggccc ccgtcgagct tcacaaggat gcagactcgt
     7081 ttctgcggct cattgatcgg gatgtcgccg aaggccaaga gctgcgtctg cacgtaagcg
     7141 agcagagtca aatcgacgtg tacttcgatg ggcaaaagca ggcaggtccg caaaatgcca
     7201 agctcgcgcg gcatgtgatc gaaatctggc tggggtatca ctccgcggca cgcagtctgc
     7261 ggcaggtgct gatcgacaag atcgaggtgc tcaagacgcc ggtgccgcga cccccgaagt
     7321 aagatcagcg ggggtttgtg gcagaggaca ttcgtttcgt cggtcaatcg gatgttgcgg
     7381 tggtttggaa gcgcccgtgc gcggcgctgc aaaacgccct ggcagacgga ttgcgcgctg
     7441 ctacgctcga tcgcgagtga gcgggaactt aacggatgga gagtggacag atgagtagac
     7501 tgctacacaa cctattttcc gtcgccagac ggcgactttc aatcgcaatg ctgctgggcg
     7561 ctgccgccag tgggctttct tcttgcgaca ccacgaccaa gcaagcggtc ggctgcaaca
     7621 ccagctcgga ctgtccgggc ggccaaatct gcggcagcac cggacagtgt ctgcccaagc
     7681 ccaagtcgct ggtgcttacg ctgacgaagc tgggcgaggg gattggcagt gtcaaatcga
     7741 ccccagcggg cattgactgc gggacgacct gttccgccag ttttgacgtt gggacgccga
     7801 ttacgctgac tgccaccgca cagagtgggt cgctggtttc gggtttttcg attggctgca
     7861 gctcgaccga cgccacctgt acatttacgc cgacgaccga agatcccgtg caggtggggg
     7921 tgaattttgc gctcgcgccc ccctcgcagc cgccgccgct gtgcaactcg tatggctttt
     7981 gctgggaaaa tccgcgaccg cagggcaaca agctcaatga cgttgcggtt ctgccgtcgg
     8041 gagaagtctg ggcagtcggc gatgccggaa cggtggttcg tcgtgtcggc accaccaata
     8101 cgctggtgag cagtggcacg acgcaggcgc tgtatggtgc ggcagtggtc ggcaccgatg
     8161 tggtgatcgt cggccaaggg ggcacggcca tgcgtggggc cggcagtgcc gtgaccacgg
     8221 aatcaagcgg ggtcgtgcaa gacctgtacg acgtgggggc gaccagcgcg ggggccgtcg
     8281 cggtgggtgc gggcggcaag atcctgcggc gcagcggcat tgcctggacg ggcgacacaa
     8341 gccccaccac ccaagcgttg cgcggcgtca gcgtgacggg aacggatatc tatgccgtgg
     8401 gggatgcggg gacggtgctg gccaactcgg gggctgcgtg gcgcgtggtg tcggacgcgc
     8461 agtttggcag caaggcgctg tcgtccgtcg ccggcctggg cggtgcggca tatatcggat
     8521 cggtgacggg cgagattttc cggcagggca gtccgtggtc gcgggtctgc tgcaacaacc
     8581 tgcccgacat caccgggatg acaagctctt cgtttgggct gctcgcggtg ggctttaccg
     8641 ttggtggttc gatcttgcag agcagcgatg ggacgacgtg gaacaccgcg gttgatggcg
     8701 cttcgacgct gctgtatggc gtggcggcca ccacggggga agcctgggcc gtcggggatg
     8761 cggggacgat gcgacgcagc acggatggtc tttcgtggcc aacgatcagc accggtgcaa
     8821 cgggactgat tcgggcggtg gctggcacaa ccgcgggcta ctacgccgcg ggccaaagcg
     8881 gtgtgctgct gcgatccgtc ggaacggcgg tgctgtccta tacggcagcg gggacgccgg
     8941 acttttttgg cgtgtcggct gcgagtccga ccgaggcatg ggccgttggc gatgtcggca
     9001 ccatctacca gttcaacgga acggcgtggc agaaagtcgt aagtggaaca ggctctgcgc
     9061 tgcgagcggt gtgggcggca ggtccagggg atgcctgggc agtcggcgac gcaggcactg
     9121 tggtgcatgt gaaaaatggc agtgcctcgt cgatgtctgg cggcacgacc caaaacctgc
     9181 tgtcggtatg gggcgtgtcg gccaccgaca tttgggcggt tggtgcggcg ggcaccgtcg
     9241 tgcgctacca aggctcgtcg tttgctccgg tgactgcgcc agccaccacg cgcgccatca
     9301 gcagcgtctg gggcaacctg gccagcaacg tctggatggt ggcggggccc gatgtctttg
     9361 tgtggaacgg caacaactat cagaaataca cgccgtcgct ttccgacatg accgcggtgg
     9421 gcgggtttag caccgaagtc tacgtgaccg gcagcaaagg ccagctttat cgctacgctg
     9481 gacccggctt tacgtcggtt gaaaccggca cgcgcaacaa tctctatggg attgcgcttt
     9541 cggcgacgag catgtggctg gtgggcgaca acgggagcgt gctgagcaag ctgcgctagc
     9601 tacagatcct gccagtcggc ggggcgctcg atcgggcaca ttccggtcaa gacttgcagg
     9661 cgcctcgcga cttcatcggc ttcggcttct aggccttcgc caatcaacca ctcaatttcg
     9721 ccgcgcgaat cacacagcga acgcaccacc gcaagaccgt cgaatgcttc gacgatcatg
     9781 cggacgtaag cgatttcagc gaggggaacg cggaagtaac gggaaggcaa ggggagggga
     9841 gccgactaga agtttacgcg gtagcccatg gtgccaaaca caccgttgag cgatgcatag
     9901 tcggcacgct cggcgcggcc ccagatgatg tggcggtagc cagcttcgac gaagaccgag
     9961 ccgcggcgca gacggatttc agcaccgagc aagccaccga ggccaacctg gctcatccac
    10021 gagccatcct gaatctggat gccgtcgcgg gcgaggaagc tgtattcggc gaggaactgc
    10081 ggcatgacac cgatataggg ggcccaggcg gcatccaggc tgcggaagcg gtacgcgagc
    10141 agcacgccac caccgacgat gtgcgaagca ccgggggcgg gcgcaaagta ctcatactgc
    10201 ggacgcagcg agaacgcgaa ggcaccatac ggcagcttga tccggccacc gatttcgagc
    10261 gcgccgccaa cgccgaacac accacgcggg atgtcgacgt gtgcaagcgg catgaggccc
    10321 agttccaccg caaacgactt cgtgggcgga cgatcggtcg aggccggctc gtctttttgc
    10381 tcttccttgc cggtcgtcgg aaccgcatcg ggcagggccg attcatagtt ttcgacgccg
    10441 ttgatggtgt agcgcagacc caggccggtc ttgcccggct gccagtgcgc cgtcgtcacg
    10501 taggtgccgg tgccgggaag ttcacgcact gcatccgcgc gcgagccacc cgtgccttcg
    10561 gcggtcacgt ttgcgccggt gatcgggctg cccgccgcgt ccgaaagctg gaggcgaact
    10621 tcgcgcgaac catcctgtgg ggtgaaggcc ggggcgccga catcgagctt ggccacaacc
    10681 gggccgctct gcgtcgccga gactttgaat tcgctgctgt cttcaggttt ggcaccgctt
    10741 tgggccgtgg cgcagcgcag gttgtgttca cgcgccggct gaagcggcat cggctgaccg
    10801 actgcctgaa gttcaccgcc gtcgagaccg cagaacacgc ctttgccgtc gacctgaatg
    10861 gcggcgcgct tgccttcacc acctggccac agcacaacgc gagcgacctg gacgtgggcg
    10921 ggagcgctcc acgggccgac cgtgccttcg gcgctgactg cgctgacccg gacaaagtag
    10981 tcaccggggc cgactgccga cggcatcact cgcgtttcgg tggcgggaac gcggccatct
    11041 ttgaccatcg tttcaaacgc cgcatcttgc gcgacttgga cttgccagct cgccggggcg
    11101 gctccgcccg cagccgcgta ggagcctttg acttcgacag ggccacccgt ggacaaaacc
    11161 aggccgggaa cacccgtgat cgtcggggct gccggcagcg gcgctgcaac gctcgggggc
    11221 gtcgtcaggt tcgcgatggt gctgctctgg ttcggggtca ggttgatcgt cttgcccttg
    11281 ccagcaagcg ccgccgcgcc gtcgtacagc gaaacgcgcg tcgcaccagt cttcggttca
    11341 accgaaatgc gagccgcgct gcctggattg acggtgactt tgcccactgg cgtcacgatc
    11401 atcgcagtgc gcggaacctt gaccggagcg ggcttggccg ccggtttttt gccagccgga
    11461 gccgccgcgc caagcacggc aggggctgcg ggaaccacca cggtgatttc accgaccgtg
    11521 atggtgttgt tgccgggggt gaacttcttg gctggaccct tggccgcagc cggtggcgga
    11581 acgccgtaca ccgaaaacga cgtgttttcc cccacgaaga cgcgggtgcc gtcgttgaag
    11641 ctgacttctg caccagagcc cgcggcactg tcgacgcgcg tgcctggcag gaccacttca
    11701 tccttgcggg cggcgtgccg gtcggtgctc gaaacgactt ccacttgatg atgcgagctg
    11761 cgcagcttgg cctcggggac accaccggga taggctgcca ctgccggagc gggagctgca
    11821 gccttggctg ccggagcggg agctgccgtc ttggccgctg gtgcgggcgc cgcagctttt
    11881 gctgccggag ctttcgccgc accgccagcc gcctgagcat agagcggctg ggtcgggatc
    11941 agaagcgcag cgcctccgac cagtacaccg aggaacaaca ttcgcgagca cgtcattgaa
    12001 ctctccaagg gacgttttgg ctcttgttct gttagccagt tcctaatttg cctgaatgca
    12061 cggagccctg tcatcaacta acaggcgagg tcgctacatc atcacattcg gcggtcaagt
    12121 caaggttcca ctttgtcaaa actcagggtc gcccatcgcc ggtgtagata cgcggtacgc
    12181 gcttggagac gacggtgcag atttcccacg ggattgtatc tgcccaactt gccatctcat
    12241 cgagcgtgat ccgaccactt tggccgtccg gtccgctttg gctgccgagc agtaccacgt
    12301 cgtctccgac cttgacgtca ggcacatcgg tgacatccac catcgtcatg tccatcgtga
    12361 tcggggcgaa cactttgcaa tggcggcctc cgcacaagac gaaggctcga tcgctcattc
    12421 tgcgggtgta gccatcggca tatccgacgg ggagcgtggc gcagatcgac ggacgattca
    12481 cccggctgcg tcccccatag cttacggttt cgccggcggg cagcttacgc agtgcaatga
    12541 tcttggtgcg cagcgtcatc accggccaca gcgtggcaag ctgggtatga tccggtgcga
    12601 cgccgaacat gccgatcccg gcgcgaacca gatcgaaccg ggcgtcggca aagcgcacgg
    12661 caccggcggt gttggcaacg tgaacgattc cgggacgcac gccttggctt tgcagcaaag
    12721 atagcgtctg tgacagtcga cggagctgtt cttccgtcgg tgcggaatct gcgccatctg
    12781 catcataaag atgggtgcac aggccggaca gatacagccc gttgtgatgg ccgagctccg
    12841 acaaaaaggg atcaagctga ccgtcgcgca ttccgagccg cgacatgccc gtgtccagtt
    12901 ttaggtggat tccgacacgc ggggcggaca gctcgtcagc cgcacgggca aagcgcgaca
    12961 gatcacggac atcggcgatg accggcgtca accgataggc gatgacatcg cgatgagaca
    13021 gaccgtagta gcagccgagc acgacgatcg gctgctcaat gcctgcgcgg cgcagctcga
    13081 cgccttcttc gaccagggaa acggcaaagc cccagacctt tttttcgagc gcgcgggcgc
    13141 aagcgacggc tccatgtcca taggcatcgg ctttgagcac ggcgagtaga ccggttttgg
    13201 cgctgcccac ggcgcttcgc agcacatcgg cattgcggcg cagcgcgccc aagtcgatct
    13261 cggccaccgt cgggcggatg tccgtgcgta tgtcaaatac ctgatagatg agcggacgat
    13321 atcgcggcgg ctagcagggc gtcaaaatgt tgcgtgcgga ctgccgcggc ttcgtcggcc
    13381 atgctaagta gtgaaaccat gtctcgactt tctgtgtctc agattggggc ggtgcttgtg
    13441 gctctttcgc tggggtcttc gccggcctgg gccgacgcac cgtggctggg tttggtgctg
    13501 caaccgggcc cgcaaggcgg agcgcagatc gttgatgtgt atcccgactc agcgctcgcg
    13561 ccccttgtaa aggggcagaa ggtacagctt ggcgaaacgg tggtgggcat cgacggcaag
    13621 ccggtgagca aacccaaaga gttgtccgag cgggtgcaaa agctcaaggt cgggcagatc
    13681 gtttcgctca agcttcagcg ggcgggcgga cctgttcacg aagtgcaagc aacgctgacc
    13741 gcgcgtccca gtgaacagct gctggaacac caagcgatga agatggaaga ggaacggcag
    13801 aagcggatga tcggacatcc ggcaccggag tttgttgtcg agcttctgca tggtccgaag
    13861 cttccggcag cggcgctggc gggaacggtg gcggcgctga aaggccagcc gattgtgctc
    13921 gtgttttttg cgagttggtg cggtccgtgt ctgcgcgaaa ttccgcagct gaccgcgatg
    13981 caagcgctgc gtccggatgt gcgggtgctc gcagtgtcga cggaagaaac cgacaaaatt
    14041 cgcgatgtga tcacacgcta tgcgcctcaa tattcgatcg gtcgcgatct cgaaaggcgt
    14101 gcttacgggg cttttggcgt cgcgtcctat cccacgacat ttctgattga tggcgcaggc
    14161 atcgtgcgag cagtcgatca cggaagtttg gacaccgtac aagcagcgct cgacaagatt
    14221 ccggcgccaa acagtgcccc ggccaagtaa tcggctacat accgtccggg gtcgtcgctg
    14281 cgtcacctgg atcggccaga tcggcggcgc tgcttagatc gaccgcactc acactgctta
    14341 gatcgatggc tctgggaatc gcgcagatcg cggtgccatc gggcaagtga cagtagccat
    14401 cgctccggca ttcgtattcc gctggacact tgggatcttc gctggcgcag cggaatgcac
    14461 agtcggaata cacgggggtg aagcagccac tgacgaccat tcccgtggcg aagctgccga
    14521 tgatcacggc ggcgagcgcg attcgtttca tcaaaacctc ccgagcagtg ccagtcccgt
    14581 ggtgtttccc gtcacaacag gaaacaggga cagttttttc tgcgcggact cggtgcggct
    14641 ctgtccataa aacagaagcc cagttcctat ggcggcaacg ccaccagcaa cgaaaaaacc
    14701 aatcgttgcg ctacggaaca aaagaccttc ctgctggagc cgttcatacg tgctgcgctg
    14761 atcggcgtcg tacaacggtg ggattccatt ttccgattgc acggtcagtc ggctcagatc
    14821 atccgatcga ttctgcgcca gcacaccgag cgtggtgccc gtcacaagaa gtcccgcggt
    14881 cagcgctgcg ctggcaaagc ccagtccgga aaagatccgc tgtcgtcggg caagcgcttg
    14941 tttttcggct tgcagcttgg ggtctggctt gggttcgatc ggatcttcgc gcagcgcttc
    15001 cgggggcggc tctacttcga gcggcggcgg ctctgtttcg gttgggggtg gttcgacttc
    15061 ggcggcgcgg acaggtgcgg taccgactgc tgctggcgtc gcttgtaccg cgggtttccc
    15121 agacaccgct tgggctggct gggcagactc tttgtgcgcg cgccgatgat gacgatggtg
    15181 tgaccctgcc gcgtttgcga catcgggccc caccaaaagg agtgcgagaa tcagcgcacc
    15241 actatacgcg ccacggacca tgtttagtgc gccgtcatcg ggttgtgttc gacgattttt
    15301 ccgtcggcaa tgtagacgac gcgcttggcg tgctgcatca cccgcggatc gtgtgtcgaa
    15361 aagataaacg tgacacccat ctgctgattg agccgctgca tcagcgaaat gatctgatcg
    15421 gatgtcttgg aatccaagtt cgctgtcggt tcatcggcca gcaccagctc tggatgggtg
    15481 atgagcgcgc gcgcaatcgc tacacgctgt cgctgaccgc ccgataattc gtctggccga
    15541 tgcttcatat acggaccaag cccaacgtct tcacagagct tgaccactcg ctgccgcagc
    15601 gatgaaagct cttccggatc ttttcgcatc agacacggaa actcgacgtt ttcgctcacg
    15661 ttcagaaccg ggatcagatt gaaagactgg aagatgattc cgactttgcg attgcgcaga
    15721 tcggccagct tgtcaaagtc gcggttgccg acttcctgtc catcaaactc ataggttccg
    15781 ctcgtcggtg aatccaaaca accgatgatg ttcagcagcg tcgatttgcc cgaaccggac
    15841 ggacccatga cgaccgtaaa ttcaccgcgc tgcacgtcga gatccacacc ctggagcgct
    15901 ggcacaatcg tctttcccag gtggtattcc ttgacaagat tgcgaatcac gatgagcggt
    15961 tttcccgact cgctttggct catgacagtc ccctagtaaa aagcccgcgc ttcaaacagg
    16021 aagcggaaat ccatgggcag aagtgagtac tcgctgtagc tcacgtcgtt tatgttgacg
    16081 gttccaaccg gaatgccgcg aatgggaatg aagccgaaca gagacagcgt aagccattcc
    16141 tgtgcattcc atgacaccgt cggtgaaatc gttcccgaca gatccgaaag accggcaatg
    16201 agtgtcgcgc cgatcgaaaa atcatcgcgg attttcggct tgttgtacga aagaaacaga
    16261 tagtggcgcc gcagtggatc gaacgtgaaa cgctgtggca ctgcgccggt tgtcccgccg
    16321 gttggctggc tgatggtggt gattccggca gcctgcgccc gcgccagcaa acgcaagtaa
    16381 tcttcaaatt ccagcgcgga atatccgtcc ccttggtagt agtactcaag cgacagcgtg
    16441 ctttcatcgc ggaactgggt ccggccccca accagcagcc gtggatacaa attcccatcg
    16501 gtcagcttgc tggcagacag cgccgtgctg ggattgcaga tgcccccatt tgcacaggca
    16561 tggtttgcaa acagcttgcc gctgccgaac tggaacagcg cttcgaaatg cagttcatag
    16621 tccgtaaaaa agtagcgcga aaagctgcca ccaaagcgcg acgtgttttc aaaggagtcg
    16681 ttgtacttgt tcgaaaagaa gtagaacaag ttgatgtcgg tgttaaaaag cagaaggtac
    16741 agccgcgccg caaacagata gtggtacgca tcgtccgctg gcttggttcc gtcggaaagc
    16801 tggtagtctg gatacttcat catcgcaaac ggaagaccgt tttgctggta caaaacctgc
    16861 ggcgcaaaca gcgtactgat cgtgaacttt tcaaacggca gctcgatccg tgcgacccac
    16921 tggccggccc gctggaagtt cggatcggtg ggatctttgg ggggatttat caggtcggtg
    16981 ggattgaacg cgaagcctga tccccagatg atgcgctttt tcccgacgag cagattgagc
    17041 cagtttttgg gcgcgaccga tacatacagt tccgatggaa caatgaacgg acgcagcgtc
    17101 gatacatcgt ggtcatcgat tttgacgcga tcgccgttct gatcgcggtc gtaatacagc
    17161 cagccgtttt gatagatcaa ggacacatcg gcgtagacaa aagccttctg tccgatgctc
    17221 actttgagct gaatgttgcc ttccagaatc tctagcagag acggctgatt gatcgtcgga
    17281 accagtgttt tcgacgggtt gatgtacgca tactggaagc ggttgtcgac atacccgttc
    17341 acatcggcag agattttttt cccggtctct gctttcttgg ttccgtcgat gtcatcgagc
    17401 gattcctggg cgctggcact tgtgcaaaaa agtccgcaga gcagcccgca gaggattccg
    17461 aagaggagtc ctgtgcggag tctgcgtgcg accccttttc tgccaaattg cagcgccaaa
    17521 gacgacatgg cttaccgtcc cagatcgtcg agaacgaact tctgcgcggg aatgtctacg
    17581 tttagcttca tgtccagcgt ggtcatttcc gaaaagcgct tggtggccag ctcattgcgc
    17641 atcgtgatgt gggaaggccg ctccagacca tggaagtttt tcacatcctc gaacaccgcg
    17701 gaacggagca tctttccgga cgcagcatag tattccgctt tggccggctg ctgcttgtct
    17761 ttggtcatcc acagcttgat gtgatcgtag gccgcttcct tgtttttcgc cgtcagatcg
    17821 agcagccaca gcttgtcatc tcccgacgga atcagcttgg ctacgtagtc ggcgacatag
    17881 tttacgcgca gcacgtcacc gttgttaaag tcaccgccct ggaagctttc gcgcgatgcc
    17941 aagcgcagcg ctcgcttgag gttgggcatg taaacccaca tgttgtcttc gacgcgcagc
    18001 atctcttgac ctttggcggc tgcgggttcg taaaaccaag tgcggatttt gtcgtcttcc
    18061 gatttcagcg cgcggaattt gtacgtccgc gtggtgtcgt cttgacggtg tgcgaccatc
    18121 accaaaagac catcaaacgt ctttggcgac atcacttcat cgtagcgttt caggagctgt
    18181 tccggggtcg gatcagcgaa cgccacagac ggtgaaagcg aaagcaaaag cagagctgaa
    18241 gcaaaggagg tgcgcagcat gggaagactc cttatgtggc gcgcagtgca tcggtcgggc
    18301 tcagccgcga tgctttgtac gcaggataga gggctgacag aaccgcaccc gtgatcgaaa
    18361 aaccgatcac gatgagcatg aaggatggtt gcacatgcgg atacgtccac agaagatcgc
    18421 caccgggtgg acgcatcgga atgccgcgct gacccagcca cagcacgaca acataaccca
    18481 aaagtgaacc gaagacagag ctggtaagac caagtgccat cgcttccaga agaaacagag
    18541 ccgtgacttg ccagcgacgg acacccaccg cgagcatcgt tccgatttca cggacccgtt
    18601 cgtacacaga catcatcatc gtgttcacga ttccggttgc gaccagcaga aacaacacca
    18661 ccgcgatgaa gatcaggaca taacccaaag acgacacgcg cgtcctggtc gccggatcaa
    18721 tgtcttccca cgtcgtgact tgatagtcat cacccagctc cgtgcgcagc cgctgcgcca
    18781 cgtcattcat ctgttccagg ttatgaacgc ccacgacgta ttcggtgact tgtcctttca
    18841 tgcgcagcag ctcttgggca aagcctacct gcacggtcgc catccgcttc gattcaatca
    18901 ggttcagcgt tggtaaataa ccacttactt cgatgtccag cgcattggta ctggcgtgcg
    18961 gacccgcagc ttgcatgacc agcgtggacc ctggatttgc cttgagtgaa tcggacagcg
    19021 tcttgccaat cagcgtcatt cctcgctgct gttgcgtaag cggagtcgat cctttggcga
    19081 cccgcttggg ccgttccgga cagactttgt attccagatc cggttcgatc gcggtggcga
    19141 caaacatggt cgattccgca ccgtttgaca ccatcccatc gaatgcaatc cgcggcgcca
    19201 cttccaccac gcccggcacc ttgcggatgc gcgccatcag cgccggatcg tgcggcatgc
    19261 tctttttcag cggatcatct gatcccaggt atcccttgcg gtagacctgc accgctccca
    19321 gcttcgacat cacaatcgct tcgatgttgt tgcggatgaa gccatttacg aagccttgca
    19381 gaaggacaat cgccatcgca ccaaacacga tcgcaagcgc ggtcagcagc gttcgccgtt
    19441 tgtttcgtgt cagatttcgc agtgccagaa gtacaagtcg catgtcacac cgccgagagc
    19501 gcttcgaccg ggcgcatccg gctcgcacgt accgacggcc acagcgcggc aatggcggcg
    19561 cccccggctg cgatggcgag aatggaaaga atgtaactga gtttcagcgt tgggaagacg
    19621 tagagcggaa gcagagatcc cggaatgtgg agcaccattc ctttgtgtcc gtagtacgcg
    19681 acaaatgacc caccaaccag cgcaccagag attccgccga gcagtccgag taccgatgct
    19741 tcgagcagaa acagcgtcaa gatttgtcgc cttcgaaccc caacagacat catcgttccg
    19801 atctcgcgcg tgcgttccag aaccgacatc agcatcgtgt tggtgatgcc aagcagcgcg
    19861 acaaacagga acacgccagc gatcaagttc aggatgaagt tctgcatcgc aatcgcatca
    19921 tcgacatacg atgcaatgtc gtgccacgtc gacacttcat actctgaacc gagcgcggcc
    19981 tgcaaaaccg gtttcagtgt ttccgcatcg tcgtagtgat gaattccgat cgcaatttcg
    20041 gtcgcgcgtc cttccatgcg cagcagttcc tgcgcaaagg gcagcggcac aaagccaagc
    20101 tttttgtccg gcaaagggag tccgggctgt ccatattccc cgacgacatc gaagtcgagc
    20161 gcgttcatga cgccatctcg atcgcctgtc agtacggttg cacgctgacc gattttgacg
    20221 cccaagttgg cggccagttc gggcgacaga accgctgcgg ttggacctga gccgcgcaga
    20281 cctttgccac ggtagatcat gtcattgcgg cgtggacaga cttcgacttc atgcaccgga
    20341 tcgatcgctg tgaacatcgc cgcggtgctg atgtcattgg cgttcgccat gccaccaaac
    20401 atgatgcgcg ccgtcaccga ctttacgccg ggaaccgcag caatcttggc caaaaatgcg
    20461 gcgtcactgg gaatcgacat ctccagagtg gcattgacag actttaggaa tccggctttg
    20521 tgaacttgca gggcgccggt ctgtccctgc acaaccgact cacgcagagt cgcttgcagg
    20581 ccgtttagga agccgcggat gctcaccatg attccgagtg ccagaaacac cgcggaaaag
    20641 gtgatgagcg atcgccgccg gttgcgaaac acgttgcgaa gcgcgatacg gagcagagga
    20701 ggaagcatgc gagcaccaca cgctgaactt ggtaatcgta agcgttcccg agatacttca
    20761 atctgtcaac gaaatttact tcagcttgtg aaataccagc ggttttgata cgcttttttt
    20821 atggcggaag tcccccagat caccaaggat cttgcgcaaa agttttttgc tcttttgctt
    20881 cgccatcgcg cctattttca ggggctggtg caagagcttg gactgtcccc accgcatgtg
    20941 ctggtgatcc agcatctgtc ggatggggca ctgacgatgc gagagctgtc tgtgcgcacc
    21001 gtttgtgaac cgtcccatct gaccgggctt gtcgacaagc tcgaagaacg caagctggtg
    21061 atccgcaaag tcgatccgaa cgatcggcgc agcaagctgg tgtcactgac gcgaaccggg
    21121 cggacgtttc gggacaagct gatggccaga atgggggcgc cggtgccgtg gatgttcacg
    21181 ctgtcggaag cggatcaagc ccagcttctg tcgatcatcg aacgagtgct cgcgcagacg
    21241 gacggcaagc tgtagtcggc gcgctgtcag cagatccggg gaagctgttc gcccgccggc
    21301 cacgcaacga tgcggcggcc tcctagctcg gtgcgcagcg aaatgatcgg cggcagatcg
    21361 gtctgttcgg tcaccgaacc gatgcgtgcg gccagccgtc cttgtggatg actgcgcatc
    21421 accgccagca aacgatcggc agcatgcgcg gcacagatcg ccaaaagtcg cccttcacat
    21481 gccagatgca gtgggtccaa accgagcagc tcgcaggcgg cacgaacggg tctttcgatg
    21541 ggaatcgccg acgcttgcag gtccatgttg acgcgagcag tttcggcaat ttcatggagc
    21601 gctgccgcca caccaccgcg ggttggatcg cgcagtacat gcaagtccgt ttcaacaccg
    21661 cgcattgcag aaaccaagct gtgcagcggc gctgtgtccg atgtgacatc gcagtcaaac
    21721 gcgattcctt cacgctggga caaaatggcg atgccgtgat tcccaatcgg accacttacc
    21781 aagatggcgt ccgtcggtgt ggcgcgagtt ggggcgatgt gaacagcggg cggaatgatt
    21841 cccaaacagg tggtgcttat gaacacgcca tcggcttttc cgcgctcgac cactttggtg
    21901 tcaccgctga cgatcggaat cccaacctgc gcgctggcag atgcaatgct cttgcagatg
    21961 cgatccagat cgcgcagcgg gaatccttct tccagaataa aaccgatggt gagcgcaatt
    22021 ggatctgcgc ccgccatcgc gacatcattg acgctgccgt tgacggccaa gtccccgatg
    22081 cagccaccgg gaaagaacag cggcgacacc acataggaat cggtcgatac ggcgatgtgt
    22141 ccgccgggca ccggcaacgt cgcagcatcg tgcgcggcgg aaagggcatc ggactgaaag
    22201 tgacgggcaa acagctcgga tacaagctga gcggtcgcgc gtccgccgct gccgtgcgga
    22261 agttcgatcc gtccgtctat caaatcgagc ggtcgcaagc gatggctaga catccgaaca
    22321 tcctgggcga gacgtggcgt gggcgcgcgc agttgaaacc tcgcgacgac gagtcctgcc
    22381 gatctgaaag tacgcggcgc aggcaccttc ggacgacacc atgcaagaac ctatcggagt
    22441 atcgggcgtg catgcggctg caaacagcgt gcattccgtc ggtgcgcaga ttccgcgcag
    22501 cacatcagga cagcggcagt gtttgtgatc cggaacctgc ggagtcgata gcgaaaagcg
    22561 cttctcagcg tcgaagtcag cgacctgctg cctggcccgc agcgcactgt gcgcaatgtc
    22621 acccagccca cgccacgcaa acgactcgcg gacttccagc acctgttcga tgagctgctt
    22681 ggctcgcagg ttcccagctt gcgtgacggc gcgggaaaat gtgttttcga cgtgggcggt
    22741 tccagcgttg atgttgtcga tcacggcatg cagcgcgagc agcagatcgg ttggttcaaa
    22801 cccggcaatg gcaatcggtt tgcgataccg ctgtgcaaaa ggcaaaaacg catcgcttcc
    22861 cgtcacggtg gcgacatggc ccggaccgat aaagccgtcg atgcggactt gatcctgggc
    22921 ggtctgcaaa agcgccgtca gcgcgggcgg ggtcagcaca tggtttacaa gcacagaaaa
    22981 gttgcgaatc ggttcctgct tggcggtcaa catcgcggcg gcggtcatcg gggtggtcgt
    23041 ttcaaaaccg acggcaaaca gcacgacctg tcgcgtggga ttctgtcttg ccaccgcgag
    23101 cgcatcttcg gtcgaataca ccacccgcac atcggcccct tgcgcacgcg ccgacagcag
    23161 acttttgtga ccaagcgcgg ggacgcgcag catgtcgccg tagctgcaca aaatgaccgg
    23221 ctcgcgcagc accaagtcga tcgcttgctg gattcgcccc gtcggcagca cgcacaccgg
    23281 acagccgggc ccgtgaatca tctgaatcgc cgcaggcagc ctgtccaaaa gccccgactg
    23341 acagagcgcg tg
//
LOCUS       DAORGN010000062        23112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_874_length_23112_cov_6.246650, whole genome shotgun sequence.
ACCESSION   DAORGN010000062 DAORGN010000000
VERSION     DAORGN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_23112_cov_6.246650"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKL17_09020"
     CDS             complement(<1..1099)
                     /locus_tag="PKL17_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK44910.1"
                     /translation="MGPSDPSLYPHLIYLVSDDDPTEVVRLCEALGENPSYLVKTFRS
                     PSLALSALRSEPIAPPLLICEQNLRELDGISLLAKVRSLSPDTVRVLLSQHADKQTML
                     RAINDGGIFQCIEKPWDRESLLLTIRNAIERGDMVVQLKRTLGAMQHNNSALSQALRQ
                     IHSAQERLLESERMAAVGRVASGIAHEIGNQLAVLSYAEMIRDRYPDDPEVKLFTSAI
                     LTARARLGGLVGEIRDFSRVHGSLPPQQDQLTVHPRFALTPEPVVLSIQEALSILRFD
                     PEFRQRTIDKDLSDNAVASVNRDKLVQVFLNLLRNALDATQPGGSLSVRVKSDPLVKE
                     PGQSSAMVRIQIDDYGAGIPSEIMPRIFEPFF"
     gene            complement(1251..2432)
                     /locus_tag="PKL17_09025"
     CDS             complement(1251..2432)
                     /locus_tag="PKL17_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44911.1"
                     /translation="MSYKSLLVAPLLFLIGTGCRTENTEGQPDQMINPEDLSVVDDLS
                     VSLPDLANTPPDLIGADLTAPPLPSDFFVVRVGDGTATLSSAATATFLERRKLSDGSL
                     VGAPLPLPVAVSGANRIFTLSGSSTAEGGLSRSSDGRYLVMAGYDATVGTAAVASTAS
                     ATTSRVIARIDTNGTIDTSTAADFFSAASVRSAHSTDGKSLWAAGAGGIVAATFGSTA
                     KPTQILTNNMRWLHISGAQLYASSASGTNVGISTIGTGLPTATATATLLPGFSAQPTS
                     SHYGFVAFDRDAVAGIDVIYVADDRAAGSGGGIQRWKLSGTTWMLDGTMAKTLTAGVR
                     GLFGYEQAGNVVLYATTAENPARVVRFTDDGTAIDMLTPTALATAATNTAYRGIALSP
                     Q"
     gene            2549..4060
                     /locus_tag="PKL17_09030"
     CDS             2549..4060
                     /locus_tag="PKL17_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK44912.1"
                     /translation="MRTPKTRLSCLLAGLFVWMGCSGDGLLAGGASDDMMPSDPIPSV
                     VPPPVITNGASDKFLLVGTLLKKDGAERGELLVESNLITCVGTGCSATSATQTATKIE
                     TGGIIMPGMLDGHNHGLFNIFDEEDWNPGKFYGSHNDWTSDVRYQQVLDAKQYLNRDV
                     TSPVDLRCEIDKYAEIKALISGTTSFTLAPGAADLACYSSLARTIDTSRNGLGVDKLR
                     TSISVPSAATAQTVCTSFANGSCNAYAVHVAEGINATAKNEFATLESRAGGCLLAPQT
                     AIVHGTALGNAEFTKMAATGMKLIWSPKSNLFLYNDTTRIDQALLAGVSTIALAPDWS
                     LGGSVNLLDELKVAQSVSQNKFSGMLTPKQLFLMVTLDAARALGVDLMLGSLEVGKRA
                     DLVLVRKQADDPYAALLAASGRDVDLVMVDGKPLYGTLELKTAASSSPVCDELSLCGQ
                     TKFLCVAESSTLNKLNQTYQEIVDTLTSNLSAYDSTVGMGAPLTPLAPLLRCP"
     gene            complement(4110..5012)
                     /locus_tag="PKL17_09035"
     CDS             complement(4110..5012)
                     /locus_tag="PKL17_09035"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNK44913.1"
                     /translation="MSKKTKTSGTPSVVNRDGTFDVLRIGMPEKPLNDLYHQLIGMGW
                     VKFISVIVLFYLCTNSVFATLYWIDPGGIENARPGSWIDSYAFSVQTMATIGYGKLAP
                     VSPFCHIIVVFESLFGLMTSAVMTGLFFSKFARPTARIMFSRVALMTVRDGQRVLLFR
                     VANERGNQVVEAQLRLWLFRTERTAEGEPVRRFYELPLVRNVSPVFAMTWTATHVLDE
                     KSPLADATTESLEATAIELVVTFIGIDNTLAQTVHARHGYRSQDIRFGERFVDMFGVT
                     ESGRRLIDYGNFHSTVPDTTPWKS"
     gene            complement(5034..6323)
                     /locus_tag="PKL17_09040"
     CDS             complement(5034..6323)
                     /locus_tag="PKL17_09040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK44914.1"
                     /translation="MRSFAAGMHVLYLTSEFLWPPLHGGRVRSLSQLRLLLAQPELSM
                     LHVFSLSEVPVSAADLDALRSALGNPAQLGIGTPVFHPIHLRQHKRKLIEVGLRRLFS
                     GTPYLLAKWRSPSVEAALSAQLTSRPWDIVYIDHLGMAVYLPLVRRLCPRARVVVESH
                     NIESEFFAQFAASKPQPLRLFAGLEADAAADHEARVLAEADAVVAISDRDAQGLRKLA
                     LRKTGRAVQPLVVPPVVEISPVEMPAHLPPRLVYVGNLTWHPNVAGLDWLCQCVFPKV
                     RELLPAATLQIAGSGLSHDDTGKLEVPAQWQAPGIEVVGFVPSLSQFVAGSPVMVAPV
                     FGGSGVRIKLLDSLRLGIPTVTTRDGASGLPLSDDIELRICDDPAEFARAVVALCQDS
                     SLRARLRNAGLAFLSEHHSPKHAQTALRMAMGLPVLG"
     gene            6399..9050
                     /gene="xdh"
                     /locus_tag="PKL17_09045"
     CDS             6399..9050
                     /gene="xdh"
                     /locus_tag="PKL17_09045"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HNK44915.1"
                     /translation="MAEISFTLNGQPQTESISDDTSLLSLLRERCAITSAKDACAPSG
                     QCGACVVLVDGEKKTSCAFPAAKVAGRSVVTLEGLAAHEKDALGSAFAESGAVQCGFC
                     IPGMVMCGKHLIDKNPTPSRADISRALSGHICRCTGYVKIVDAIEQAAACLRGEGLPP
                     CDRSGQIGTALPRFRGKEMALGEFRYVDDLSAPDMLHGALVLSLHPRAKVLSIDVSAA
                     QSLPGVRAVVTAKDVPGQRYQGLIYRDWPIFVAVGEETRYVGDVLCAVAADSRELARL
                     AASLVVVNYEVLTPLSSPEAALAKDAPLLHPENGHADNLLSTSKVVRGDVAKGEAESA
                     VIVEETFRTQFIEHAFMEPESCLSVPQGAQDIDGTVTDRLHVYSQGQGVYDDQRQLAS
                     ALGMSEDALRVTLVSAGGAFGGKEDLSIQAQTALLSLVTGKAVKLTIDRVESIRLHPK
                     RHPLTMRYRVGCAADGRITFVKVRLIGDKGAYASVGSKVIERACGHATGAYDVEHVDV
                     EGLAVYTSNPPCGAMRGFGANQANFAMESALDMLAEKLGLDAWDIRSRNALVDGSTFC
                     SGQKLRAVGLRKTLDAVRPHYLAARAQGKSVGLACGIKNVGIGNGMADIGRAALLVNH
                     DGTVTVANGYTEMGQGLFTILIQVASTVTGLPPSTFVATVDTADKVPSGMTTASRATV
                     CAGRAVQDAAQKLKAALSEKSLADLAGQRFDGAFIFDKTSPLGADVKDPITHLSFGYA
                     TQLCIVDETGKVERMVAAHDVGRALNPKLLSGQIEGSIHMGLGFALTEELIVENSEIK
                     NPTLRGIGLIRAEPMPQIDVLLIEEPEPDGPFGAKGVGEIGLVPTAPAVANALYQFDQ
                     QRRFSLPMRDSVAARALHPQPKRAARS"
     gene            complement(9053..>9805)
                     /locus_tag="PKL17_09050"
     CDS             complement(9053..>9805)
                     /locus_tag="PKL17_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HNK44916.1"
                     /translation="VPTVLLQLGIALTGNYGYFNWLALLLALLLVPPSWLERLRPNPA
                     ADDDAESHQPARPSLLFGLPRLAISTLLFSASLGHFLWTLRFRTDVPRPIHQAMKLVA
                     PLQAVSHYGPFASMTQSRPELVIEGSIDGQTWREYPFRYKPGDLQRSPRWNAPHQPRL
                     DWQMWFAALGRPSDSPWLEILLLRLLEGSKDVLWLFESDPFAGRKPRYLRVQHYQYRF
                     TNLRQKQETGAYFTRESPLPFISPVGLTVADP"
     assembly_gap    9806..9905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9906..10396)
                     /locus_tag="PKL17_09055"
     CDS             complement(<9906..10396)
                     /locus_tag="PKL17_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44917.1"
                     /translation="MIRRIASLLRRWLLPTSLPELPLSAGRDALFVRLLGLLYVVAFA
                     SLWPQIAGLFGNQGILPTQMFLGSVAHTLGSRKYLMLPTLFWLSSDNTALQLVCGLGL
                     CAGLAIGALRLVWPMLLVAWLCHLSFLSVGGAFLSFQWDALLSEIGVIALVAVPLRYR
                     TPQP"
     gene            complement(10393..11472)
                     /locus_tag="PKL17_09060"
     CDS             complement(10393..11472)
                     /locus_tag="PKL17_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44918.1"
                     /translation="MVQTGLQRRPAGALRFWLLGSLLVTPLAGSAAPPTARPQLEKSE
                     PLPSEPIAIAKLSFSGMAEEVSSDLRSSLATTVQRGGYSVRSPQEVDSLLGSELRLLG
                     CTTASCYGRLAQLLGVRRVIEGEVQRLELSTFSLRLQMRDLFSGQSTTPIQERCDVCS
                     TDDVRQMVVRAAERLTKEVPPRGPQMTEPRIGESGILIVETEPPGATVTIDGAQRSEK
                     TPASYLLAAGVHSLVVQDDKHRRVRQQVEVAPGSQPMTLRLSLSAQNTRRSWMTGLGI
                     AATVGAVGLIAGGAALLYKHNSPVFTADCPDLGLSDYHCPSKYDNLAPGVGMLVGGGV
                     LAITGGVLLYLDSRPTKAKPVVEKQ"
     gene            11676..12197
                     /locus_tag="PKL17_09065"
     CDS             11676..12197
                     /locus_tag="PKL17_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNK44919.1"
                     /translation="MNAGLHSFDTASPVALIEHILRRYHQPLPGQVAALCTQAQSLQK
                     NPNEPVALLAQVLRLLNELWAELEPHLLKEERVLFPMIEAVVVAKESGRPPLPSPSQL
                     AMGPIRVMRMEHDRADEILRALAELTTHYQAVDGASEPLRALYAGLKQLDDELREHIR
                     IENDLLFPAVTRL"
     gene            complement(12222..13253)
                     /locus_tag="PKL17_09070"
     CDS             complement(12222..13253)
                     /locus_tag="PKL17_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNK44920.1"
                     /translation="MSKAARGVILSSAAGAALYIVLAAGYGEGAKVWAELGRFSPGLL
                     LIGLLLASVNYLFRFVRWQYYLRLLDVPTWPHKNDPSEKTAESHVPLFDSLLLFLSGF
                     ALTVTPGKVGEVLKSYLLRESFGQPIARTAPIVLAERLTDLIGLLLLTLLGVSTWMRP
                     EHHYLVGLGMSVCVLLTLLASWQKLVYAVLGLFEKIPSLGPKVAPKLREFYDAAYLLL
                     RPVPLLLATLTSIGSWFAECLAFYLMIRGFPDGGQASITLATFIYAVMTVAGALAFVP
                     GGLGVTEGGMALLLAELLSMSRASAIAATLIIRIETLWFAVLLGVLALLWFSRRRHMQ
                     VSIDDVRAK"
     gene            complement(13317..14036)
                     /locus_tag="PKL17_09075"
     CDS             complement(13317..14036)
                     /locus_tag="PKL17_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HNK44921.1"
                     /translation="MNRRCAIKMCLAVTAWGLSAPASATEHEALSFGSGGCGKLQGGE
                     FLPCSGPNFESFAKSACALGRNALHPLVRDTVVDAYRSLAHSHPDRRWQYGEMGNAKG
                     GKLWPHKTHQNGLSADFFVPVIAKDGAPALVPISVFHKLGYGLSFRKDGTLDDLRIDF
                     RAIGAHLLAIESAGRAHDVVIERIILTPDFHQMLFAQAPETKRLAPRFMKREAWVRHD
                     EHYHVDFGIPQRLLRPLSCAR"
     gene            complement(14084..15466)
                     /locus_tag="PKL17_09080"
     CDS             complement(14084..15466)
                     /locus_tag="PKL17_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44922.1"
                     /translation="MNRLRESKSVWGLGIALSVLLSGCAKRPYYSGFYFDSPPLVETD
                     RSEMVRYLDAVLESRALKKVTLVTPEKGVQSLLPRDETSPGTTDWIPLPLLFAARFVL
                     SPKYRGLEVDLLAKTEAEGYTPVSAIHELDGSVRLEMGPSFRDLVPENDDQFGRDALC
                     KRYHIGGIDDGNRNWTKLELYALGQALSLLRPDELAVLRGVHFVRNARAVESSKGLAL
                     HKVRAQYHGTVHTGDGHKDAREIHLFDTNPAHDTSLFVGEPSHPYPIPTMALLHEIGH
                     AVADYVRIQAYWRFNQTVDSYNALVAAWNQSIQDRAVTESARAELDLRLALAKKEGEL
                     ARQALDAVKKQERQGNGSVLAAFRTARGSEKGPTEYGGTDVEESFAECFALAKADPQA
                     LKRIYPPLADWFADAEHLRPLLPLLPPQKAAPQNPDVKVNPSTPAQKQTPLAQETESF
                     AQPASRDGTE"
     gene            15582..17639
                     /locus_tag="PKL17_09085"
     CDS             15582..17639
                     /locus_tag="PKL17_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44923.1"
                     /translation="MASALYVARCLSVDGATVSIELQIVHPDISEFGTDRLFAWKLLH
                     EWVSPADDGTDVELASRYVKSVKLSPLRNAKKKHVGKDFYTVTRVGEQKPSVTYTIVV
                     TEPLLLSGIKAGDSDEVYDFVEPGKGDALVPPPKASKPKPSKPKKAAAAPDIPVKTYA
                     DLRQLALEGIRKHGGSWSSYLIENGMIERWAQTERDAQILSYLEFLTPEHRVAAACVV
                     ALTRLRARQTEAAKALLSYAESHYQASEDYNWSAGGGLCALWWRLQKESQADALFARI
                     ETEQTERKTNHNQAVHVRFSILAQGGKWDRAEQLIPSLLKDGSYRADLLVPGVLAAWE
                     DHPSLFAKVMDHWQMRADERSMSNYELSTDLCRREVARGTPEKYLASALRWHHVLDGS
                     TAAAIALLRTEQNDPNRAIADAQTVLSTPQLGSYVQEYGLAVLTRHARPLADRFVEQN
                     LPTWLRDGYGHGFLAAADRTELLGKHLENHPVYRPASVARLLHDRDAAIAMIKQAMTA
                     DMQLGGSHFVDLVDLGERAFVDEQLRSALLRLAKEPTRQRDLPCRSLAMTAAQVGMPE
                     HGFAAIKLPGPALRRYSASDMVSGAVSVRDFGTAYAAAMLVPDDDSNGRVSSLIQGVL
                     RGADEQFLQGPRALPLSVRDVDYARALCAAGERDAVAWMLSAHRQTLSDAEKRAISEL
                     LAK"
     gene            17761..18198
                     /locus_tag="PKL17_09090"
     CDS             17761..18198
                     /locus_tag="PKL17_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK44924.1"
                     /translation="MPAPTTTAASSKGTVLIADDDPEILMMLSLRLSKKGYKVFEAAD
                     GLQTIQIAREKQPDLVLLDVMMPGKNGWEVARELRNDPQFANVGIVMLTAIGEKVNEM
                     TSPLYGADEYVDKPFDFTDLESKIARVIAARHGSKSAPKGSTK"
     gene            18282..19823
                     /locus_tag="PKL17_09095"
     CDS             18282..19823
                     /locus_tag="PKL17_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK44925.1"
                     /translation="MLGLAIGLCGAFSAAEAKKPAKPLSAAATKRTEQLAEQGLAALK
                     AGQFAEAVQSFVDGFRVSPLPKWLFHLAQVAAAEKKPVEARDLLRRFLADDTLEADDP
                     LRKDAQALLDSLQVVDAGEILVVGPRGAWLSVDGRFVGSLPLSQPLLVPTGPHRVVAS
                     QDKWQASSEVRTRLARQVELRFKSGSDVAVVTLPPAVLVIDSAPDAQSAEAVKKKVQT
                     ALSRESFAMVPRSAVETYAKDIANCLTDVTCLRKAGQRFGVEFVLWASTVKQGSGWKV
                     EIKLRDTATGTELGQQSAQCDSCAIDGLLPKVGETTVALASNASSRDRSDLEVRSNPS
                     GAEVLVDGQSLGSAPLKQSLLSGQYALVVRKPGYIDFSQQIEVKGSGPLVIDAQLTDR
                     DAQNEPSPSARPEPVRTVGEQKRPLWRYVAGGSAIGVGVILLGFGVSAFVQNGVVLNG
                     ECPASIGAQRCQFGTVVPGIGLTVAGGLLASAGAALIAWPAPKPQTQLSLFGATNGMA
                     LELRY"
     gene            complement(19846..21195)
                     /locus_tag="PKL17_09100"
     CDS             complement(19846..21195)
                     /locus_tag="PKL17_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK44926.1"
                     /translation="MNIHRLCVGAVFGLGLCSVTAGRAQEKPPDAMRIVTLRQAIEMA
                     RKHQPQLLQARAQSEAARARIDQARAPILPQLVGTASYQRTTANFVARPGAVPAQFAN
                     MAAATSLDTFNYFNFGLQLSQYIWDFGQTTRKWQAAKTSAEAQSATEKVTLLQITAAV
                     HSAYFAARAQKALLKVAADNLANQQRHLVQVQAFVQVGTRPEIDLAQARTDRANASVQ
                     VIASENNYAVAKAQLNQAMGIEADVDYDVQDEPFVAIPSEEESTQVLLAEALKHRPEF
                     VALSRQIEAQKLTLAATKGAYGPSLNATMNLTDAGTDITQLSWNWNAAVNLNWPIFQG
                     LLTHSQVREQHANLSGLEAQLATLRQQVRVDIESSRLQVRAGKAAWQASEEALKNASE
                     RLRLAEARYRTGIGSIIELGDAQVAMTTAAAQRVSAEYQLASARVLLLRALGLTATE"
     gene            21500..22810
                     /locus_tag="PKL17_09105"
     CDS             21500..22810
                     /locus_tag="PKL17_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK44927.1"
                     /translation="MHSIVPNLRTAENRLADARHCARRRLLSCLSLLLALGLGPACKS
                     KDSSAPADAVMSRPVPVLLAPVARRDLPIYLDGLGTVTAFKTVTVRTQVDGRLEKVLF
                     REGQRVSRGELLAQVDPRPFLATLHQAESALARDEALLANARKNLQRYKDLRTQNLLS
                     QQQLDDQQALVSQYEGSVGVDRAAAEAARLNVEYSRIVAPIDGVTGVRLIDEGNLVQK
                     SDATGLVVITQIDPIAVLFTLPEDDLPKIIAQLPPNQTAPSAKTEPGTAADKTPTTPK
                     GQPVEAWSRDGTTKLGVGELLVLDNQIQQGTATLRLKAVFGNPHRQLWPNQFVKARLL
                     LSTQHDALVVPAVAVQRGPKGTFVYVVGDEGTANPRPVDVALLSGDQAVLAKGVKPGE
                     QIVIEGQNQLRPGAKVSARPAAGGSADRSDGERDKTGRGGGHKP"
     gene            22807..>23112
                     /locus_tag="PKL17_09110"
     CDS             22807..>23112
                     /locus_tag="PKL17_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNK44928.1"
                     /translation="MSISEPFIRRPIATSLLMIGILLAGLAGYRELPVSALPQVDYPT
                     IVVSTTLPGASAETMSSAVTTPLERQFGQMPSLAQMTSVSSFATSQITLQFNLDRSID
                     "
BASE COUNT         4676 a         6680 c         7125 g         4531 t
ORIGIN      
        1 tgaaaaacgg ttcaaaaatg cgcggcatga tctcggaggg aatgcctgcg ccgtagtcgt
       61 cgatctggat gcgtaccatc gcggaggatt ggccgggttc tttcacgagc ggatcagact
      121 ttacgcgcac gctcaagctg ccacccggct gcgtggcgtc gagcgcgttg cgcaaaaggt
      181 tgagaaacac ttgcacgagc ttgtcgcggt ttacgctggc gacggcgttg tccgacaggt
      241 ctttgtcgat ggtgcgctgc cggaactcgg ggtcaaaacg caggatcgac agcgcttctt
      301 gtatcgacag caccaccggt tccggggtca gcgcaaagcg tggatggacc gtcagttggt
      361 cttgctgtgg cggcaggctg ccgtgcacgc gcgaaaagtc gcggatttcg ccaaccagac
      421 cacccagccg cgcccgtgcc gtcaaaatcg ccgacgtaaa cagcttcact tcgggatcgt
      481 cgggatagcg atcgcggatc atctcggcgt agctcagcac cgccagttga ttgccaattt
      541 cgtgcgcaat tcccgacgcc actcgtccga cggcggccat ccgttcgctt tccagcagcc
      601 gttcctgcgc cgagtgaatt tgccgcagcg cctgcgacag tgcgctgttg ttgtgctgca
      661 tcgcaccgag cgttcgcttg agctgcacga ccatgtcgcc gcgctcaatc gcgttgcgaa
      721 tcgtcagaag caggctttcg cgatcccacg gtttttcgat gcactggaaa atgccgccgt
      781 cgtttatcgc ccgcagcatc gtctgtttgt cagcgtgctg cgacaacagc acccgcaccg
      841 tatcgggcga caggctgcgc actttggcca aaagcgaaat gccgtcgagc tcgcgcaggt
      901 tctgttcgca gatgaggagc ggcggcgcga tcggttcact gcgcagcgcc gacagcgcca
      961 ggctgggact gcggaacgtc ttgacgagat agcttgggtt ttcgccaagc gcctcgcaca
     1021 gccgcaccac ttccgtcggg tcatcgtcgc tgaccagata gatgaggtgc ggatacaggc
     1081 tcggatcgct tggtcccatc gaaggcaaag cctgacttcc tgtgggttcg tcgctgcgac
     1141 ctgaatttcc gtcagaattt ccagcgggcg gcagcatgtg cgcggagcat acccggtttg
     1201 tggcggacag agaagccacc acgcagcgct gttttgcaga tgtgcggaag ttactgtggc
     1261 gaaagcgcga tgccgcgata agcggtgttg gtggctgccg ttgccagcgc cgtcggagtc
     1321 agcatgtcga tggccgttcc atcgtcggta aaacgaacca cacgggccgg attttcggcg
     1381 gtggtcgcgt acagcacgac gttgccggct tgttcataac caaaaagtcc gcgcacacca
     1441 gcggtcagtg tctttgccat cgtaccgtcg agcatccagg ttgtaccgct cagtttccag
     1501 cgctggatgc caccccccga tccagcggcg cgatcgtcgg cgacatagat cacgtcgatt
     1561 cctgccactg catcgcgatc aaaagcgaca aaaccatagt gagagcttgt gggctgggcg
     1621 gaaaaaccag gcagcaaagt cgccgtcgca gtggccgtcg gtaggccggt tccgatcgtg
     1681 ctgatcccca cattggtgcc cgatgcgctg cttgcgtaca gctgcgcccc ggaaatgtgg
     1741 agccagcgca tgttgtttgt gagaatctgc gtcggcttgg ccgtgctgcc gaacgtcgct
     1801 gcgacaatgc cgccggcgcc tgcggcccag aggcttttcc catcggtgct gtgcgcgctg
     1861 cgaacggatg ctgcggaaaa gaaatcggcg gcggtgctgg tgtcgatggt gccgtttgtg
     1921 tcgatgcgtg cgatgacgcg gctggtcgtg gccgatgcgg tgctggcaac cgcggcggtt
     1981 ccaacggttg catcatagcc ggccatcacc agatagcgac catcgctcga acgcgaaaga
     2041 ccgccttctg ccgtcgagct accggacagc gtgaagattc gattggcgcc acttaccgcg
     2101 acgggcagcg gcagcggggc accgacgagc gatccatcgc tgagcttgcg ccgttccaga
     2161 aatgtggcgg tggcggcact gctcagggtt gccgttccat cgcccacgcg gacgacaaag
     2221 aaatcacttg gcaaaggtgg cgcagtcagg tctgcaccga tgagatcagg tggcgtgttg
     2281 gcgagatcag gaagcgaaac agacagatca tcgaccaccg aaaggtcttc cggattgatc
     2341 atttgatccg gctgtccttc ggtgttttcg gttcggcatc cggttccgat caggaacaaa
     2401 agcggagcca caagcagcga cttgtaggac atggtttccc cctccgcagg aatcttaggc
     2461 tccgcgattc cggggcgcaa accacattcc tttgacaaag gtggcggcaa attcctgtgc
     2521 cagtcgaaaa tcaaaggagg ggggatcgat gcgcacacca aagactcgtc tgtcctgtct
     2581 gctggctggt ctttttgtct ggatgggatg ttcgggcgat ggacttctgg caggtggtgc
     2641 tagcgacgac atgatgccga gcgatccgat tccttctgtt gtgcctccac ctgtgatcac
     2701 aaacggggct tcggacaaat ttctgcttgt gggaacgctg ctgaaaaaag acggggcgga
     2761 gcgtggggaa ctgctggtcg aaagcaacct gatcacctgc gtgggaaccg gctgttccgc
     2821 cacttctgca acgcagactg cgacgaaaat tgaaaccggc ggaatcatca tgccgggcat
     2881 gctggatggt cacaatcacg gtctgtttaa catctttgat gaagaagact ggaatccggg
     2941 caagttttac ggcagccaca acgactggac atcagatgtc cgttatcagc aggttctcga
     3001 cgcaaaacag tatctgaaca gagatgtcac atccccggtt gatctgcgct gcgagatcga
     3061 caaatacgca gagatcaaag cgctgatttc cggcaccacg tcttttacac tggcacccgg
     3121 agccgctgat ctcgcgtgct attcctcgct ggcgcgcacg attgatacat cgcgaaacgg
     3181 acttggagta gacaagctgc ggacttccat ttccgttcca agcgcagcga cggcacagac
     3241 cgtctgtacg tcctttgcaa atggttcctg taacgcctat gccgtgcatg tcgcggaagg
     3301 aatcaatgcc acggcaaaaa acgaatttgc cacgctagag agccgcgctg ggggttgtct
     3361 gttggctcca cagactgcga ttgtgcatgg caccgcactt gggaatgcgg aatttaccaa
     3421 aatggcagcg acggggatga agctgatctg gtcgcccaag tcgaatctgt ttctgtataa
     3481 cgacacgacg cgaatcgatc aggcgctgct tgcgggggtc agcacgattg cactggctcc
     3541 ggactggtcg ctgggcggca gtgtcaactt gcttgatgaa ctgaaggttg cgcagtctgt
     3601 aagccaaaac aaattttccg gaatgctgac gccaaagcag ctctttttga tggtcacgct
     3661 cgacgctgcg cgggcgctcg gagtggattt gatgctgggt tccttggaag taggaaagcg
     3721 cgccgatctg gttctggtgc ggaaacaggc ggacgatcca tatgcggcgc tgcttgcggc
     3781 atccgggcgg gatgtggatc tggtgatggt ggatgggaaa ccgctgtatg ggacgctgga
     3841 actgaagacc gcagcgtcga gcagcccagt ctgtgacgag ctttccttgt gtggccagac
     3901 caagttcctt tgtgtggcgg aaagctcgac gttaaacaaa ctgaaccaga cgtatcagga
     3961 gatcgtggac acgcttactt cgaatctgtc tgcgtatgac agcacggtcg gaatgggggc
     4021 accgctcact ccgctcgccc cgcttctgcg ctgtccgtag tcgcaaaatt ccgtcggaaa
     4081 gacgattcca aaacacattc cgtgggcggt taggatttcc acggagtggt gtctggaacc
     4141 gtgctgtgga agttcccgta atcgatcaag cgacgtccgc tttcggtgac tccgaacatg
     4201 tcgacaaagc gttcgccaaa gcgaatgtct tggctgcgat agccgtgccg ggcatgcacc
     4261 gtctgtgcga gggtgttgtc gattccgatg aaggtgacaa ccagttcaat cgcggttgct
     4321 tccagtgatt cggtggttgc atcagcgagc ggactttttt catccagcac atgggtcgcg
     4381 gtccacgtca tcgcaaacac cggggataca ttgcggacca gcggcagttc ataaaaacga
     4441 cggactggct cgccttccgc tgtccgttcg gtgcggaaca gccacagcct tagctgcgct
     4501 tccacgactt gattgccgcg ctcgttggcg acgcgaaaca gcagaacgcg ctggccatcg
     4561 cgcacagtca taagcgcgac gcggctgaac atgatgcggg cggtcggtcg cgcgaacttg
     4621 gaaaaaaaca gtccggtcat gaccgcggaa gtcatcaggc cgaacagcga ttcgaagacg
     4681 acgatgatgt ggcaaaacgg actgacgggc gcgagctttc cgtatccaat cgtcgccatg
     4741 gtctggacgc taaatgcata ggaatcgatc cacgaaccgg ggcgcgcgtt ttcgattccg
     4801 cctggatcga tccagtacag agtcgcaaag accgagtttg tacacagata aaacagcaca
     4861 atcacgctga tgaatttgac ccagcccatt ccgatcagct ggtgatacag atcattcagt
     4921 ggtttttcgg gcattccgat gcgcaaaaca tcgaatgtcc cgtcgcggtt cacgaccgat
     4981 ggcgtgccac tggttttggt ttttttcgac acggtggaat catccttttg cggtcagcca
     5041 agaaccggca gacccatcgc catgcgcagc gcggtctgtg cgtgctttgg gctgtgatgt
     5101 tcggaaagga atgcgagacc ggcgttccgg agccgcgccc ggagcgagct gtcctgacag
     5161 agcgcgacga cagcgcgggc aaattcggca ggatcgtcac aaatgcgaag ctctatgtcg
     5221 tcggaaagcg gcagtcccga tgctccatcg cgggtcgtca cggtaggaat tccgagccgt
     5281 agggaatcga gcagcttgat gcggactcct gatccaccga acaccggcgc caccatcacg
     5341 ggagatccgg cgacaaactg cgacagactg ggcacaaagc cgaccacttc gatgcccgga
     5401 gcttgccact gcgcagggac ttccagcttg ccggtgtcat cgtgggacag tcccgaaccg
     5461 gcgatttgca gcgtcgccgc gggcaaaagc tcgcgcactt tgggaaacac gcattggcac
     5521 agccagtcga gccccgcgac gttgggatgc caggtcaggt tgccgacgta gacaaggcgc
     5581 ggtggcaaat gcgcgggcat ctcgacgggg gatatctcga cgacgggcgg caccacaagt
     5641 ggctgcaccg cgcgtccagt tttgcgcagc gcaagcttgc gcagaccctg tgcatcgcga
     5701 tcggagatcg ccaccaccgc atcggcttcg gcaagcacgc gggcttcgtg atccgctgcg
     5761 gcgtcagctt ccaggccggc aaagagtcgc agtggctgcg gtttggacgc ggcaaactgg
     5821 gcgaaaaact cgctttcaat gttgtggctt tcgacgacga cacgggcgcg cggacagagc
     5881 ctgcgaacca gcggcagata gaccgccatt cccaggtgat cgatgtagac gatgtcccac
     5941 ggcctgctgg tcagttgggc agatagcgcc gcttctactg acggtgaacg ccactttgca
     6001 agcaggtagg gcgtgcccga aaacagccgc cgaaggccga cttcgatgag cttgcgcttg
     6061 tgctgtcgca ggtggatcgg atgaaacacc ggcgttccga taccaagctg ggccggattc
     6121 ccaagcgcac tgcgcagcgc gtccaggtcc gcggcagaga caggcacttc cgacagcgaa
     6181 aacacatgca gcatcgacag ctctggctgg gccaaaagca gtcgcagctg gctgagcgaa
     6241 cgaacccgtc ccccatgcag cggaggccac agaaattcgg aggtcaaata caagacgtgc
     6301 atgccagcgg caaaggatcg cacagtttgc ggcctttacg tgtcggtcct tcgcgaagcc
     6361 gtggtatgcg tgagactctt ttcgaaagcg aggtagacat ggcagagatc agcttcaccc
     6421 tcaacggaca gccccaaacg gagtcgattt ccgacgacac cagcttgctt tcgctgcttc
     6481 gtgagcgctg cgcgataact tctgccaaag atgcctgtgc accgtcggga cagtgtggcg
     6541 cgtgcgtggt tctggtcgat ggggaaaaga aaacgtcgtg cgcatttccg gcagccaaag
     6601 tggccggtcg cagcgtcgtc accctcgaag gcctggcggc acacgaaaaa gacgcgctcg
     6661 gcagtgcatt tgccgaaagt ggcgccgtcc agtgcggttt ttgcattccg ggcatggtga
     6721 tgtgcggcaa gcacctgatc gacaaaaacc cgacgccgtc gcgggccgac atcagccggg
     6781 cgctgtcggg tcacatctgt cgctgcaccg gctatgtgaa gatcgtcgat gcgatcgagc
     6841 aagccgcggc ctgtctgcgc ggcgaaggtc tgccgccgtg tgatcgctcg ggacagatcg
     6901 ggacggcgct gccgcgcttt cgtggcaaag agatggcgct gggggagttt cgctacgtcg
     6961 atgatctgtc ggcgcccgac atgctgcacg gtgcgctggt tctgtctctg catccgcgcg
     7021 ccaaagtgct gtcgattgat gtcagtgccg cacagtcgct gccgggcgtt cgcgccgtcg
     7081 tcaccgcaaa agatgtcccc ggtcagcgct accaaggtct gatttatcgg gactggccga
     7141 tctttgtcgc cgtcggagaa gaaacgcgct acgtcggcga tgtgctgtgt gccgttgcgg
     7201 ctgacagccg cgagctggcc cgcctggccg catcgcttgt cgtcgtgaac tacgaagtgc
     7261 tgaccccact gtcgagcccc gaggcagcgc tggccaaaga tgcgccgctt ttgcatcccg
     7321 aaaatggcca tgccgacaac ctgctatcga catcgaaagt ggtgcgcggt gatgtcgcaa
     7381 aaggcgaagc cgaaagcgcg gtcatcgtcg aagaaacctt ccgcacgcag tttatcgagc
     7441 atgcctttat ggaacccgaa agctgtcttt ccgtgcccca aggtgcgcaa gacatcgacg
     7501 ggaccgtgac ggaccggctg catgtctatt cgcaagggca gggggtttac gacgatcagc
     7561 ggcagctcgc gtcggcgctg gggatgtccg aagacgctct gcgggtgacg ctggtgtcgg
     7621 cgggcggtgc gtttggtggc aaagaagacc tgtcgattca ggcgcagacg gcgctgcttt
     7681 cgctggtgac gggcaaagcg gtcaagctga ccatcgatcg cgtcgagtcg attcggctgc
     7741 atcccaagcg ccacccgctg accatgcgct atcgtgttgg ctgcgctgcc gacggacgga
     7801 tcacgtttgt caaagttcgt ctgattggtg acaaaggcgc gtatgcgtcg gtgggcagca
     7861 aagtcatcga gcgggcctgc gggcacgcga ccggcgctta cgatgtcgaa cacgtcgacg
     7921 tcgaaggact tgcggtctac acaagcaatc caccgtgtgg ggcgatgcgc ggctttggcg
     7981 ccaaccaggc taactttgcg atggaatcgg cgctcgacat gctggcggaa aagctggggc
     8041 tcgatgcctg ggacattcgc tcgcgcaatg cgctcgtcga cggatcgacg ttttgttcgg
     8101 gtcagaaact gcgggcggtc gggctgcgca agacgctcga tgcagtcagg ccccactatc
     8161 tggctgcgcg ggcgcagggc aaatcggtcg gtctggcgtg cgggatcaaa aacgtcggca
     8221 ttggcaacgg catggccgac atcggacggg cggcgctctt ggtcaaccac gacggcacgg
     8281 tcacggtggc caacggctac accgaaatgg ggcagggtct ttttacgatt ctgatccaag
     8341 ttgcgagcac cgtgacaggg cttcctccgt cgacgtttgt ggcgacggtc gacacggcgg
     8401 acaaagttcc gtcggggatg acgacggctt cgcgggcgac ggtgtgtgcg ggacgggcgg
     8461 tgcaagatgc ggcgcagaag ctcaaagcgg cactatcgga aaaatccctg gccgatttgg
     8521 cgggtcagcg cttcgacggg gcgttcattt tcgacaagac ctcgccgctc ggggccgatg
     8581 tcaaagatcc gatcacccac ctgtcgtttg gttatgcgac ccagctgtgc attgtcgacg
     8641 agacgggcaa ggtcgaacgg atggtcgcgg cgcacgatgt cggtcgagcg ctgaatccga
     8701 agcttttgtc ggggcaaatc gaaggttcga tccacatggg tctgggcttt gctctcaccg
     8761 aggagctgat cgtcgaaaac agcgaaatca agaatccgac gctgcgcggc attggcctga
     8821 ttcgggccga accgatgccg cagattgacg tgctgctcat cgaggagccg gaacccgacg
     8881 gtccgtttgg ggccaaaggc gtcggtgaaa ttggcctggt tccaacggca ccggcggtgg
     8941 ccaatgcgct gtatcagttc gaccagcagc gtcgcttttc cctgccgatg cgtgactcgg
     9001 tggctgcgcg cgccctgcat ccccagccga agcgcgctgc ccgcagctaa acttacggat
     9061 cagcgacggt cagaccgacc ggcgaaatga acggaagcgg cgactcacgg gtgaaatagg
     9121 cgccggtttc ctgtttctgg cgcaggttcg taaaacggta ctggtagtgc tgcacgcgca
     9181 ggtagcgcgg cttgcgtccc gcaaacgggt ccgattcaaa cagccacagc acgtcctttg
     9241 agccttcaag aagccgcaag agcaggattt ccagccacgg actgtcactt gggcgaccga
     9301 gcgccgcaaa ccacatctgc caatcgagcc ggggctgatg cggcgcattc cagcgcggcg
     9361 acctttgcaa gtcccctggt ttgtaacgaa acggatattc gcgccaggtc tgcccgtcga
     9421 tgctgccttc gatgacaagc tctggacggc tctgggtcat cgacgcaaac ggaccatagt
     9481 gcgacaccgc ttgcagcggc gcgacaagct tcatcgcttg gtgaatcgga cgtggcacgt
     9541 cggtgcgaaa tcgcagcgtc cacaaaaaat ggcccaagct ggcggaaaac agcagcgtgc
     9601 tgatcgcaag ccgaggcagc ccgaaaagaa gcgacggacg ggctggctga tggctttcgg
     9661 catcgtcgtc cgccgccgga ttgggacgca gccgctcaag ccaagagggc ggcacaagca
     9721 aaagcgcgag caaaagcgcc agccagttga agtagccata gtttccggtc agcgcgattc
     9781 cgagctgaag cagcaccgtc gggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnggctg cggggttcga tagcgcagcg gcaccgcgac aagcgcaatc acgccgattt
     9961 ccgacagcag cgcatcccac tgaaacgaca aaaacgcgcc gcccaccgac agaaacgaca
    10021 ggtgacacag ccaagcgaca agcagcatcg gccagaccag ccgcagggcg cctattgcaa
    10081 gcccggcaca aagccccagc ccgcagacaa gctgtagcgc cgtgttgtcg ctgctcagcc
    10141 aaaacagcgt cggcagcatc aagtatttgc gactgccgag cgtatgtgcg acggaaccca
    10201 ggaacatctg cgtcggcaaa atcccctgat tcccaaacag cccagcaatt tgcggccaca
    10261 gcgacgcgaa agcgaccacg tacaaaagcc cgagcagacg cacaaacagc gcatcacgcc
    10321 cggcggacag cggcagttcg gggagagaag taggaagaag ccagcgacga agtagcgaag
    10381 cgatccgcct gatcactgtt tttcaacaac cggcttggct ttggtgggtc ggctgtccag
    10441 gtagagcagc acgccgccag tgatagcaag aacgccgcca ccgacgagca ttccgacgcc
    10501 aggtgccaag ttgtcgtact tgctcgggca gtgatagtcg gacaggccca ggtcgggaca
    10561 atcggcagtg aacaccggcg agttgtgctt atacaaaagc gcggcgcctc cggcgataag
    10621 accgacggct ccgacggtgg ctgcgatgcc aagtccggtc atccacgacc gccgcgtgtt
    10681 ctgggccgac aggctcagac gcagcgtcat cggctggctg ccgggggcca cctcgacttg
    10741 ctgacggacg cggcgatgtt tgtcgtcttg caccaccagg ctgtggacgc ccgcggccaa
    10801 aagatacgac gccggggttt tttcgctgcg ctgggctccg tcgatggtga cggtggcgcc
    10861 gggtggttcg gtttcgacga tcaggatgcc gctttcaccg atccgcggtt cggtcatctg
    10921 cgggccgcgc ggcggcactt ctttggtcag tcgttcggcg gcgcgcacca ccatctgacg
    10981 cacgtcgtcc gtcgaacaga catcgcagcg ttcttgaatc ggcgtcgtgc tttgcccgga
    11041 aaacagatcg cgcatctgaa gccgcagcga aaacgtcgac agctccaatc gctgcacttc
    11101 gccttcgatt acccgtcgca cgccgagaag ctgggccagc cggccatagc agcttgccgt
    11161 cgtgcaaccg agcagtcgca gctcactccc gagcagactg tcgacttcct gcgggctgcg
    11221 caccgaataa ccgccgcgct gcacggtcgt cgccaggctg cttcgcaggt cgctcgacac
    11281 ttcttcggcc ataccagaaa acgaaagctt ggcgatggcg atcggctcac tcggcagagg
    11341 ctcgctcttt tccagttgtg ggcgcgcggt gggtggtgcg gcactgcctg cgagcggcgt
    11401 cacaagcagg ctcccaagca gccagaagcg aagcgcccca gccggacgcc tctgcaagcc
    11461 agtttggacc atgagttata agaatcgcac cgcttaggct ctgtcaatcg tcgtgcgcag
    11521 agattgggcg agaaccaagc cactcatcgt cagcacagca ccattttgga cgccttgcgg
    11581 tctacatcgt gctttggcgc caagtttggc tacttaggtc acagcgatgc gacttgtctg
    11641 cgcgtagtct gtgccgtgcg gaggtaatcc aagtcatgaa tgctgggctt cattcgtttg
    11701 ataccgcgtc gcccgtcgcg ctcatcgaac acattcttcg tcgctatcac cagccgctac
    11761 cgggacaagt ggcggcgctt tgtacgcagg cccaatcgct gcaaaaaaac ccgaacgagc
    11821 ccgtcgcgct gctggcgcag gtcctgcgac ttctcaacga gctgtgggcc gagctggagc
    11881 cacacttgct caaagaagag cgggtattgt ttccgatgat cgaagcggtt gtcgtcgcca
    11941 aagagagtgg tcgtccgccg ttgccgtcgc caagccagct tgcgatgggg ccgatccgcg
    12001 tgatgcgaat ggaacacgat cgcgccgatg agatcctgcg ggcgctggct gagctgacca
    12061 cccattacca agcagtcgat ggggcgagcg agccgctgcg ggctctgtat gcggggctta
    12121 aacaactgga tgacgagctg cgcgaacaca tccggatcga aaacgacctg ctgtttcctg
    12181 ctgtcacccg tctgtgaaca ccgtcgcttt tgtggccgcc gttatttcgc gcgcacatcg
    12241 tcgatagaga cctgcatgtg acgacggcgc gaaaaccaca aaagcgccag cacgccaagc
    12301 agtaccgcaa accacagcgt ctctatccga atgatcagcg tcgctgcgat ggcactcgcg
    12361 cgggacatcg acaaaagttc ggccaaaagc agcgccattc ccccttcggt gacgccgagt
    12421 ccgccgggca caaacgcgag cgcgcccgcc accgtcatca ccgcgtaaat gaacgtcgcc
    12481 agcgtgatag atgcctggcc tccgtcggga aaaccgcgaa tcatcaggta aaacgccagg
    12541 cattcggcaa accacgaacc gatcgaggtc agcgtcgcca gcaaaagcgg caccgggcgc
    12601 agaagcaggt acgctgcatc gtaaaactcg cgcagcttgg gtgcgacttt tggacccaat
    12661 gacggaattt tttcaaacag gcccagcact gcgtagacca gcttttgcca cgatgcgagc
    12721 agcgtcagca gcacacacac cgacatgccc agcccgacca ggtagtgatg ttccgggcgc
    12781 atccacgtcg acacaccaag aagcgtcagc agcaaaagtc caatcaagtc ggtcagtcgc
    12841 tcggccagca caatcggtgc ggtgcgcgca atcggttggc caaagctctc gcgcagcaga
    12901 tacgacttga gcacctcgcc gacttttcct ggcgtcaccg tcagcgcaaa acccgacaga
    12961 aacagaagca gcgaatcaaa cagcggcaca tgcgattccg cggttttttc tgacggatcg
    13021 tttttgtgcg gccacgtcgg cacatcgagc agccgcagat agtactgcca gcggacaaag
    13081 cgaaacaggt agtttaccga cgccaaaagc agcccaatca gcaaaagacc gggcgaaaaa
    13141 cggcccagct cggcccaaac cttcgctcct tcgccataac ctgccgcgag cacgatgtag
    13201 agtgccgccc ctgctgccga agacagaatc acgccgcgtg cagctttgct catggcggcg
    13261 aaaaatcgcc caggttgccg ccccctgtca acccagccga tcgtcggctg ggggttctaa
    13321 cgcgcgcagg acagcggtcg caagagccgc tgcggaatcc caaagtcgac gtggtagtgc
    13381 tcatcgtggc gcacccacgc ttcgcgcttc ataaaccgcg gtgcaagtcg ttttgtctcg
    13441 ggtgcctgcg caaacagcat ctggtgaaaa tccggcgtca agatgatgcg ctcgatgaca
    13501 acgtcatgtg cacggccagc cgactcgatc gcaagcaggt gcgccccgat cgcacgaaag
    13561 tcgatgcgca gatcgtcgag cgttccgtct ttgcgaaacg aaagcccgta ccccagcttg
    13621 tgaaacacag agatcggaac cagcgccggc gcgccatctt ttgcgatcac cgggacaaaa
    13681 aagtccgcgg acagaccgtt ttggtgggtc ttgtgcggcc acagcttccc gcccttcgcg
    13741 tttcccattt cgccatactg ccagcgtcgg tcgggatggg aatgtgccag cgatcgatag
    13801 gcatccacca ccgtatcgcg cacaagcgga tgcagcgcat tgcgacccag tgcgcacgcg
    13861 gacttggcaa acgactcgaa gttcggtccg ctgcatggca aaaactcgcc tccttgcagc
    13921 ttgccgcagc ccccgctgcc aaacgaaagc gcttcatgtt ccgtcgcaga tgcgggtgca
    13981 gacaggcccc atgcggtcac tgccaaacac atcttgatcg cgcaacgtcg gttcatgaaa
    14041 aatcagtctc tgctcacgat cgtagatcga tgcgtgcgga atttcattcc gttccgtctc
    14101 tgcttgcggg ctgtgcgaaa gactctgttt cctgtgccaa cggagtctgc ttttgcgcag
    14161 gcgtggaagg attcaccttc acatcaggat tctgtggcgc agctttctgc gggggcagaa
    14221 gcggaagcag cgggcgcagg tgttccgcat cggcaaacca atcggcaagt ggcggataaa
    14281 ttcgtttgag cgcctgtgga tcggccttgg cgagcgcgaa acactcggca aaggactctt
    14341 cgacatcggt tcccccatac tccgtcggac ccttttcgga acctctcgct gtacggaatg
    14401 ccgcaagtac gctgccgttt ccttgacgct cctgtttttt gaccgcatcc agcgcttgcc
    14461 gcgccagttc cccttctttt ttcgccagcg ccagtcgcag atccagttct gcccgcgccg
    14521 attccgttac cgcacgatcc tgaatgcttt gattccatgc agcgacgagc gcgttatagg
    14581 aatcgaccgt ttggttaaac cgccagtagg cttggatgcg cacataatcg gcaacggcat
    14641 gtccgatttc atgcaagagc gccatcgtcg gaatcggata ggggtgactt ggctcgccga
    14701 caaacagcga cgtatcgtgc gccggattcg tatcaaaaag atggatttcg cgcgcatctt
    14761 tatgtccgtc tccggtgtga accgtcccat gatactgcgc cctcactttg tggagcgcga
    14821 gtcctttgct gctttcgacc gcgcgtgcgt tgcggacaaa gtgaacgcca cgcaagaccg
    14881 ccagctcgtc gggccgtaac aaagaaagcg cctgacccag cgcgtacagt tcgagcttgg
    14941 tccagttccg attcccatcg tcaatgcccc caatgtggta acgcttgcag agcgcatccc
    15001 gaccaaattg atcgtcattt tcggggacca gatcgcgaaa cgatggaccc atttccagcc
    15061 gcacgctgcc atccagttca tgaatggcag agactggggt gtatccttcg gcttctgttt
    15121 tcgccagcag atcgacttcc agaccgcggt actttggact aagtacaaaa cgcgccgcga
    15181 acagaagagg cagcggaatc cagtcggttg ttccgggcga agtctcgtcg cgcggtagca
    15241 gactctgcac gcctttttcc ggtgtcacca gcgtgacttt tttcagcgcg cggctttcca
    15301 gtaccgcatc cagatagcgc accatttcac tgcgatcggt ttccaccagc ggcggtgaat
    15361 caaaataaaa accgctgtaa tagggacgct tggcacagcc tgacaaaagc acagacagcg
    15421 cgattcccaa accccaaaca gacttcgatt cccgcagccg attcatgccg catctatagc
    15481 atcaattttt tgacgcatgc gcagcgattg cgtgggtcca aaacgtccct ttgggaaagc
    15541 ttgcacgtcg aaatccccga atttgagata caatcggcgg catggccagt gctctgtatg
    15601 ttgcgcgttg tctatcggtc gatggggcta cggtgtcgat cgagcttcag attgttcatc
    15661 ccgatatctc ggagtttgga accgatcggc tgtttgcgtg gaagctgctg catgaatggg
    15721 tgtcaccagc ggacgacgga accgatgtcg agctggcttc tcgctatgtg aagtcggtca
    15781 agctgtcgcc gctgcgcaat gccaagaaaa aacacgttgg caaagacttc tatacagtga
    15841 cccgcgtcgg cgaacaaaag ccgtcggtga cgtatacgat cgtggtaacg gaaccgctgc
    15901 ttctgtcggg catcaaagcg ggggattccg atgaagtgta tgactttgtc gaaccaggca
    15961 agggtgacgc tcttgtgccg ccgcccaaag cgagcaagcc gaagccgagc aagccgaaaa
    16021 aagcagccgc ggctcccgac attccggtca agacctatgc ggatctgcgt cagttggcgt
    16081 tggaaggaat ccggaagcac ggcggaagct ggtcaagtta cctgattgaa aacggaatga
    16141 tcgagcgctg ggcccagaca gaacgtgatg cccagattct gtcctatttg gaattcttga
    16201 cgccggaaca tcgcgtcgct gcggcgtgtg tcgtggcgct tactcggctt cgcgcaaggc
    16261 agacggaagc ggccaaagca ctcctttcct atgcagagtc ccactatcaa gcgtcggaag
    16321 actacaactg gagtgccggc ggtggattgt gtgcgctgtg gtggcgtctg caaaaggaat
    16381 cacaagccga tgcgctgttt gcccgaattg aaacggaaca gactgagcga aagaccaatc
    16441 acaaccaagc ggtacatgtt cgcttttcga tcttggctca gggtggcaag tgggatcgcg
    16501 cagaacagct tatcccgtcg ctgttaaagg acggaagtta ccgtgcggat ctgcttgttc
    16561 ctggggttct tgctgcgtgg gaagaccatc cttcgctgtt tgccaaggtg atggatcact
    16621 ggcagatgcg tgcggatgag cggagcatgt ctaactacga acttagcacc gacctgtgtc
    16681 gacgcgaggt ggcgcgcggt acaccggaaa aatacctggc gagtgcgctg cgctggcatc
    16741 acgtactcga tggatccacg gcggcggcga ttgcgctttt gcggaccgaa cagaacgacc
    16801 cgaatcgcgc cattgcggat gcgcagacgg tgctttcgac gccacagctt ggatcgtatg
    16861 tccaggaata cggccttgcg gtgctaaccc gtcatgcgcg gccacttgcc gaccgctttg
    16921 tcgaacaaaa ccttcctacc tggctgcgcg atggctatgg acatggtttt ctggctgcgg
    16981 cggatcggac cgagctgctc gggaaacacc tggagaatca tccggtctat cgtccggcga
    17041 gcgtggcgcg tctgcttcac gaccgcgatg ctgcgattgc gatgatcaag caagcgatga
    17101 ctgcggacat gcaactgggt ggttcgcact ttgttgattt ggtcgacttg ggggaacgcg
    17161 cttttgtcga cgaacagctt cgcagcgcac ttcttcgttt ggccaaagag ccgacgcgcc
    17221 agcgggatct tccgtgtcgt tctttggcca tgacggcggc ccaagtggga atgcctgaac
    17281 atggctttgc cgcgatcaaa cttccggggc cggcgctgcg tcgttattcc gcgagcgaca
    17341 tggtaagcgg tgcggtgagc gtccgcgact ttggaaccgc ttatgctgcg gcgatgctgg
    17401 ttcccgacga tgacagcaat ggccgggtca gttcgctgat tcaaggtgtg ctgcgtggcg
    17461 cagacgagca attccttcag ggtccgcgcg cgctgccgct ttcggttcgc gatgtcgact
    17521 atgcgcgtgc gctctgtgcc gcgggggaac gcgatgcggt cgcttggatg ttgtcggcgc
    17581 atcgtcaaac gctatcggac gcggaaaagc gcgccatttc ggaactgctc gcgaagtaag
    17641 cgggcggtgg acctgggatg tggacaaacg cgcacaccgt cagaacgaaa gccgcagtcg
    17701 gtgatttttg tggcggggga acacttgacc taccagggcg gccaggctag cgtgtctgac
    17761 atgcctgcac cgacgacgac tgccgcctcc agcaagggta ctgttctgat cgcggacgac
    17821 gatccggaaa ttttgatgat gctgtcgctc cgactgagca aaaaaggcta caaagtcttc
    17881 gaggctgcgg acggacttca gaccatccag atcgcccgcg aaaaacagcc ggatctggtg
    17941 ctgctcgacg tgatgatgcc gggcaaaaac ggctgggaag tcgctcgcga actgcgcaac
    18001 gacccacagt ttgccaacgt cgggatcgtg atgctgacgg cgattggtga aaaagtgaac
    18061 gagatgactt cgccgctgta tggcgccgat gaatacgtcg acaagccgtt cgatttcacc
    18121 gatctcgaaa gcaagatcgc gcgggtcatt gctgcccgcc acgggagcaa gtccgctccg
    18181 aaaggttcga ccaagtaaac gacaaaacct ctgctttcgc gatttcctct ggtctgtcat
    18241 aatgaaaaga cttgggaagc gcgatgagag gggctgcgcg tgtgcttggt ttggctatcg
    18301 gactctgcgg ggcgttttct gccgcagagg ccaaaaaacc tgccaaacca ctcagcgctg
    18361 cggcgacaaa acggaccgag cagcttgcag agcaaggtct ggcggcgcta aaagcgggcc
    18421 agtttgccga agctgttcag tccttcgtcg atggctttcg ggtcagtcca ctgcccaagt
    18481 ggctgttcca tttggcgcag gttgcggcgg cggaaaaaaa gccggtcgaa gcgcgcgatc
    18541 tgctgcgccg ctttctcgct gatgacacgc tggaagcgga cgatccgctg cgcaaagacg
    18601 cgcaagcgct gctcgattcg ctccaggttg tcgacgcggg tgaaattctg gtcgtcgggc
    18661 cgcgcggcgc ttggctgtcg gtggatggtc ggttcgtcgg gagcttgccg ctgtcgcagc
    18721 cgctgctggt tccgaccggg ccgcatcgcg tcgtcgccag ccaggacaaa tggcaagctt
    18781 caagcgaagt gcggacgcgg ctcgctcgcc aggtcgagct gcgtttcaag agcggttccg
    18841 acgtcgcagt ggtgacgttg ccgccagccg tgctggtgat cgactcggcg cccgatgccc
    18901 aaagtgccga agcggtgaaa aagaaagtcc agaccgcgct cagtcgggaa agtttcgcga
    18961 tggtgccgcg cagcgccgtc gagacctatg ccaaagacat cgccaactgc ctgaccgatg
    19021 tcacctgttt gcgcaaagcc ggacagcgtt tcggcgtcga gtttgtcctg tgggcaagca
    19081 ccgtcaagca gggctcgggc tggaaagtcg aaatcaaact acgcgacaca gcgacgggca
    19141 ccgagcttgg acagcagagc gcgcagtgtg attcgtgtgc aatcgatggg ctgctgccca
    19201 aagtcggcga aacgacagtg gcgctggcca gcaatgccag cagccgtgat cgcagcgatc
    19261 tagaagtgcg atcgaatccg tccggggccg aggtgttggt cgacggacag agcctcggca
    19321 gcgcgccgct caaacagtcg cttttgtccg gtcaatatgc gcttgtggtt cgcaagccgg
    19381 gctatatcga cttttcgcag cagatcgaag tgaagggcag cgggccgctt gtgatcgatg
    19441 cgcagcttac cgatcgcgat gcgcaaaatg aaccttcgcc gtcggcaaga ccggaacctg
    19501 tgcgaaccgt tggcgaacag aaaagaccgc tttggcgcta tgtcgcaggt ggttcggcga
    19561 tcggtgtcgg tgtgatcttg ctcggctttg gcgtaagtgc atttgtgcaa aacggcgtcg
    19621 tcctaaatgg cgaatgtccg gcgtcgattg gggcccagcg ctgtcagttt ggcacggtgg
    19681 ttccgggcat cggtctgacg gtggcgggcg gacttttggc ttcggcgggg gcggcactga
    19741 ttgcgtggcc tgcgcccaaa ccgcagaccc aactgtccct gtttggcgcg acgaacggca
    19801 tggcgctgga gctgcgctac taacatcgca agcagcgcta cgcgactatt cggtggccgt
    19861 cagcccgagc gcgcgcagca agagcacgcg ggcgctggca agctggtatt ccgccgaaac
    19921 gcgctgtgcc gccgcggtgg tcatcgcaac ttgggcatcg cccagctcga tgatgctgcc
    19981 gatgccggtt cgatagcgcg cttccgcaag ccgcagccgt tcgcttgcat tttttaacgc
    20041 ttcttcgctg gcctgccatg ctgctttgcc ggcgcgaact tgcaggcgac tggactcgat
    20101 gtcgacccgc acttgctgcc gcagcgtcgc aagctgggct tccagtcccg ataggttggc
    20161 gtgctgttca cgaacttgcg aatgggtcag gagcccctga aaaatcggcc agtttaggtt
    20221 caccgccgca ttccagttcc acgatagctg ggtgatgtcg gtgcccgcgt cggtcaagtt
    20281 catggtcgcg ttcagcgacg gaccgtaggc tcctttggtg gcggcaagcg tcagtttttg
    20341 cgcttctatc tgccgcgaca gcgcgacaaa ttcaggccga tgttttagcg cttccgccag
    20401 cagcacctgg gtactttcct cttcggaagg aatcgccaca aacggttcat cttgtacgtc
    20461 gtaatcgaca tcggcttcga ttcccatcgc ttggtttagc tgcgctttgg caactgcgta
    20521 gttgttttcg ctggcaatca cctgcaccga cgcattggcg cgatccgtgc gggcctgcgc
    20581 cagatcgatt tcgggccgcg ttccgacctg cacaaacgcc tgcacttgca caagatgccg
    20641 ctgctgattg gccaggttgt cggctgccac tttcagcagc gctttttgcg cccgcgccgc
    20701 aaagtacgcg ctgtggaccg ccgcagtgat ctggagaagc gtcacttttt ccgtcgcgct
    20761 ctgtgcttcg gcgcttgttt tggcggcctg ccatttgcgc gtcgtttgcc caaagtccca
    20821 gatgtactgc gacagctgga gtccaaagtt aaagtagttg aacgtatcga gcgaagtcgc
    20881 tgccgccatg ttggcaaact gcgcgggcac agcgcccgga cgggcgacaa agttggccgt
    20941 cgtgcgctga tacgatgccg tccccacaag ctgcggcaaa atcggtgcac gggcttggtc
    21001 tatgcgcgcg cgtgccgctt cactttgcgc ccgcgcttgc agaagctgcg gttggtgctt
    21061 tcgcgccatc tcgatggcct ggcgcagcgt cacgatccgc atcgcgtccg gtggtttttc
    21121 ctgcgcacgt cctgccgtca cgctgcaaag cccaagtccg aagactgcgc ccacgcagag
    21181 cctgtgaatg ttcatgctgt taactctcat cttcgtcggg acggctgtgt aagccgtgtt
    21241 cctccagctt ggcgtacagg tgcggacgac cgaaaccgaa tgtcggaccc ataacaagct
    21301 tccatctggc tgcctacgac aaacgctgag catgccgcaa ccggacggcc cagttggctg
    21361 atttttcgtc ggaaagccgc tcgctgtcgt ttcatctgca gctatcgctg gctgcggatt
    21421 tgggcggtcc cggcatgttt cacggacttt tgcgcagcca gggatggaca gggcccagcc
    21481 attgctgctt tgtttcggca tgcattcgat cgttccgaac ctgcggactg cggaaaatcg
    21541 cctggccgat gcccggcatt gtgcgcgtcg ccggctgctt tcttgcttgt cgcttctgct
    21601 tgcgctgggt ctgggtccgg cgtgcaagtc caaagactcg tcggcgccgg cagacgctgt
    21661 gatgtcgcgg cccgttccgg tgctgctggc tccggttgcg cggcgcgatc tgccgattta
    21721 tttggacgga cttggtacgg tgacggcgtt caagacggtg acggtgcgga cgcaggtgga
    21781 tggccgcttg gaaaaagtgc tgttccgcga aggccagcgg gtttcgcgcg gcgagcttct
    21841 ggcacaggtc gatccgcgac catttttggc gacgctgcat caagccgaaa gcgcgctggc
    21901 tcgggatgaa gcgctgcttg caaacgcgcg caaaaacttg cagcgctaca aggatctgcg
    21961 gacgcaaaac ttgttaagcc agcagcagct cgatgatcag caagcgctgg tcagtcagta
    22021 cgaaggatcg gtgggagtcg atcgcgcagc ggcggaagcg gcgcggctca atgtcgaata
    22081 ttcacgcatt gtggcgccga ttgatggcgt gacgggcgtg cggctcatcg acgaagggaa
    22141 cctggtgcaa aagagcgacg ccacaggcct tgtggtgatc acgcaaatcg atccgatcgc
    22201 ggtgctgttt acgctgccgg aagatgatct gccgaagatc atcgcgcagc taccaccgaa
    22261 ccagaccgcg cccagtgcga agacggaacc agggacggct gcggataaaa cgcccactac
    22321 gcccaaagga cagccggtcg aagcgtggag ccgcgatggc acaaccaagc ttggggtggg
    22381 agagctgctc gtgctcgaca accagattca gcagggcacg gcgacgctgc ggctcaaagc
    22441 ggtgtttggg aatccgcaca ggcagctctg gccgaatcag tttgtcaaag cgcgactgct
    22501 tttgtcgacg cagcacgatg cgctggtggt gccggcagtg gcggtgcagc gcggtcccaa
    22561 aggaaccttt gtgtatgtcg tgggcgatga ggggacagcg aatccgcgac ctgtggatgt
    22621 ggcgcttttg agcggcgatc aagcggtgct ggccaaaggg gtcaagcccg gcgaacagat
    22681 tgtcatcgaa ggacagaatc agcttcgtcc gggggccaag gtttcggcgc gaccggcagc
    22741 gggcggaagt gccgatcgca gcgacgggga acgtgacaag acaggccgcg gaggcgggca
    22801 caagccatga gcatttccga gccgttcatc cgtcgcccga ttgcgacatc gctgctcatg
    22861 ataggaattc tgcttgcggg acttgccggc taccgcgagc tgccggtgtc ggcgctgccg
    22921 caggttgact atccgacgat tgtggtgtca acgacgctgc cgggggccag tgcggaaacg
    22981 atgtcatcgg cggtgacgac tcctttggaa cggcagtttg gccagatgcc gtcgcttgcg
    23041 cagatgacgt cggtgtcgag ctttgcgaca tcacagatca cgcttcagtt caacttggat
    23101 cgcagcattg at
//
LOCUS       DAORGN010000063        23083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_877_length_23083_cov_5.258989, whole genome shotgun sequence.
ACCESSION   DAORGN010000063 DAORGN010000000
VERSION     DAORGN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 23083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23083
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_877_length_23083_cov_5.258989"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKL17_09115"
     CDS             <1..201
                     /locus_tag="PKL17_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44929.1"
                     /translation="TTLDLPATVRRDSLLSVDAGITKTLPAGFSIDLTVTFMRNVSNI
                     ANGIDNRNYSKLTTMLGIYYSF"
     gene            215..1258
                     /locus_tag="PKL17_09120"
     CDS             215..1258
                     /locus_tag="PKL17_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNK44930.1"
                     /translation="MRADPTLLADDLCELLHRTSGFPEDQTVAKLLCATPDAVDGALA
                     EVASRGLLPSLVSTMLRAEDDALLGAVTMFAKSPGVRVALCHEVLQVEDQDVQAALER
                     LLISATDQLPTVFSQLVDQGDYEVLLNALRVTESADTPTLRALAAARPTYGMDQLNAS
                     ALHRLTSSAKQTPYDLLIVPGFTPVNAKQPIHLRDLPPAQARIQLAAQDLKSGLARAV
                     LVSGGSVHPPGTPYNEALMMRSGLLELGIPAEQILVDPHARHSTTNLRNAGRAMLSLG
                     MGRGLIVTGFDSAPFSQAFYFAHANLSTFNLRCQHELGYSVGTLNERDAHHVEFSPSG
                     DVTRKNYADPLDA"
     gene            complement(1262..2041)
                     /locus_tag="PKL17_09125"
     CDS             complement(1262..2041)
                     /locus_tag="PKL17_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNK44931.1"
                     /translation="MTTVSDAPFGKPLDDVREETGAPSSTSTDAPSPPYTLAIDIGGT
                     GIKCLLLDSAGQPSGKRQRKETPNPATPDAVLAVIRSLLPDTTFDRVSVGFPGVVKKG
                     VIFAAPNLDASFVGFDLQSALAKMTEKPVSVLNDAGLQGLGVIEGHGTEIVVTLGTGM
                     GFALYIDGHYVPNVELAHHPLTKQRTYEENVGNRARKRLGKRKWEAQVRKVIKQIQKT
                     FNPDKLYVGGGNAAKYEGKLPDGVVKIDNIAGLLGGIALWR"
     gene            2165..3415
                     /locus_tag="PKL17_09130"
     CDS             2165..3415
                     /locus_tag="PKL17_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNK44932.1"
                     /translation="MLPAFPTLRRKLDLFPCLSLALLALAGGCAKPELVRSPTQVVSY
                     LADGDAQLVQSVPQGTELLDPTLPTTQLVWIEMIRSAKRTIDWACFYVASSRGQALDP
                     VLSELSQAAARGVRVRMIFDKQMAKTDPQALAFLLRMPGAAIRVLDFNLIATGSHHAK
                     YLVVDGRELFLGSQNFDWRSLSQIHELGVRLAQPHIVGQLAFLFEMDFALAQDRNDTL
                     ESNPPVLPRGPTPEIEVGISPPQLTPDGIRPALPVLLDMIRGAKSTLSIQLLRYSAHS
                     GTDHWDTLEEALLMAASRGVKVQLLVSNWSIQSPEIDDLKKLSQVPNIEVRIASIPEL
                     PGQFIPYARVIHSKYAVADEHQLWLGTTNWERGMFMASRNVDAVFRRPVLAKQAQQIF
                     NRLWKSSYSAPVDPSKDYVPPRVR"
     gene            complement(3428..3853)
                     /locus_tag="PKL17_09135"
     CDS             complement(3428..3853)
                     /locus_tag="PKL17_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44933.1"
                     /translation="MIHIRAGLCFGILLALSHAGCDGTSTGATDAAVDATNTTMTAYA
                     QSCDLRPTMKANMLYCQEFVTDATILTSYKMSCASSGGSWSTVGCNRTGALGGCKTTS
                     NVGLSTVTQISWYYIGGYYPSADSIKQSCASSNGMYLAP"
     gene            complement(3879..6365)
                     /locus_tag="PKL17_09140"
     CDS             complement(3879..6365)
                     /locus_tag="PKL17_09140"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNK44934.1"
                     /translation="MTQSLSARTLGCGISVWLLLASQGVRSEEEYGDRRKVPRNPQNA
                     CEVADSNLQKAGDAILRDAQRPVAVAASQRWDKRTTPKYLDQFERRFTLRPSERALLY
                     KQGMVSLARLSFESYVTALHEIYQSQLPLYVSIDALLHAVYASNDGLMADLEERIAVP
                     NIIKFLTETRAKLPSFQQAISADTAHDLDLYLTVAARLLGDKHPPLFQVTENEASSLL
                     AQIRSAKDLTGVSLFGRPRMIDFSQYAPRGHYAAEPQMTALFQTAMWLSRLEFNLVSR
                     SSRSSAPGTSPDPRETPREVALALALANLMQQANVTAEVRALDELWSFLAGRREDVSL
                     PELLVLQKQAGITDLRAESLSDKLRTAIGNRFGRTVSMHYMPQGSVNLPVIATVLGPR
                     VVSDAQRLRPLVHDAVPDKYRLHFSEVAYALGHDRALSYLTPELAKFPSLKKNLDLSR
                     TMVSVPSPAQPRTDLYSAWLGSVETLAAKPSGVHPSFMDSDAYADLRINSTAAGYAQI
                     KHNYVLVAGQPYDLGGCEIPDGYVEPAPETLRGLREYARRGVQAATALDPEDKTAARS
                     YFQRLDRLLAILSVIVDDELAGRTLTANQKRFLSMVVEMAPGSSGGPPTFTGWYFDLF
                     RKRAADGLGRSQLVADYYTSTNLGEASYVGIADVRLGLFVVDTNGGPRIMTGPIARAY
                     ETHQPMGGTASRLDDLASGELPETARKDPWAQSYTVAAPTDIPSLLISYDPDASPDIA
                     VETEAPVSDVTITLLDHHRQPMASLQRSFPKGKTVFRFPARSPSGRSLVGSQVHGLSV
                     RRGEFFTWKALMRAGMENGIYLPIGPKSEN"
     gene            6428..7186
                     /locus_tag="PKL17_09145"
     CDS             6428..7186
                     /locus_tag="PKL17_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNK44935.1"
                     /translation="MSVAQTAKIPLRHDVCDAYECLSGDADSPLLLTCEHASQRFPSG
                     YALQARDNRLVDTHWAFDLGARELTHELASALSATAVLANFSRLLIDPNREEHHPDLL
                     RTTADGEAILLNQNVTASDRLHRTVSYYRPYHEAVDAALARSNASVLLSIHTFTPIYE
                     GQPRTVELGVLFDRDESQAESLLSSLRAVFPHVAANQPWSGKLGLIYSAESHAHRFGR
                     TALELEVRQDRAQDPSYRQRLIAVLCDHFRHSSI"
     gene            complement(7239..7667)
                     /locus_tag="PKL17_09150"
     CDS             complement(7239..7667)
                     /locus_tag="PKL17_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44936.1"
                     /translation="MKRMILAALLLGCVNDDIRLYETQVMGSVSATGKGTLHLEFHHA
                     KTFGSGALSHPLGLFDSRKVTLENLPYRFDETLLYPEQKGEGLVVYGWLDTDGDGVLC
                     MPGKPAEPAGLVRVSDFPSHQVHTTLVLDENCAGPETLYP"
     gene            complement(7664..8356)
                     /locus_tag="PKL17_09155"
     CDS             complement(7664..8356)
                     /locus_tag="PKL17_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44937.1"
                     /translation="MLTTAVLALLALQAETNQPPPTENPSEQTSQPVITLLLAPDPVV
                     LDQLAGGPQKGMWGVSVQAGYPWFSLRTMYGVGHRLAPLFELETALGRRFTTSLGLSL
                     AWVNRPHFRLSGEVLLGWMFQEGELARRGPSGELRLRAAFSVGRLIPYLVLGTRHAIL
                     PSRTQIERESGVDTTWTVRHEWTPWGTLGLGIRATTSLGVDVAIDYGWVDAPGSVAIP
                     GAHVGLHFGGGR"
     gene            complement(8364..9386)
                     /locus_tag="PKL17_09160"
     CDS             complement(8364..9386)
                     /locus_tag="PKL17_09160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK44938.1"
                     /translation="MMRRLRSCLLVGVALLALGCDTPRGVDWDGRRLPVVDMHLHPGE
                     WDAIPETTRTFLGSRFPFPLNLNPKSTAEGILSAEGILAELDKAGVSQAVLFAVYSPR
                     TVGIATNERVLSDTAKAPDRLYGLASLRVDNFRTDKDAELNRLRAALSNPSMIGIKLA
                     HAHQHFRMDDPTFFGIYQVASDLKKPLYLHTGSSPFPGTSSEPPYTDPTYLEAAIQSY
                     PGAVFILGHLGYDFGDQQPGSLDACIRLAKTYPNVYLEPSALGSKGSDPTGENLKYAM
                     QKLRDAGLVDRIIYGSDGPQSPGFVKDYLSRTVTAMKASGYTADEAAAVFSENFARVF
                     GVAKAK"
     gene            complement(9383..11653)
                     /locus_tag="PKL17_09165"
     CDS             complement(9383..11653)
                     /locus_tag="PKL17_09165"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44939.1"
                     /translation="MKAYTWVLSALPLVSDGAKALALCSLSFSFLPASAHAGSTDVGP
                     LRALRDVVQPTQPMFSSPDTATLVTVSAAQRLVGSDAATGDYEGASVSIDGDTAVVAA
                     PYAAPMGRTDAGAVYIFVRNGTTWTQQAKIVAADGRNGDNFGAKVALRGDTLVVGAPG
                     ADQFGRTDVGAAYVFARTGTTWNQQAKLTAFDGGSDDQFGFAVALDGDIAVVGARGAD
                     VMMRKDAGAAYVFGKRGPNWAAFGKLTASDAQAGDSFGVGVALSGDTLVIGADFADPS
                     GKSEAGAAYVFQRGPMGFSQSAKLLAADGQSGDWFGRQVSVDGTTAVVGALYGDSGGK
                     VNSGSAYVFTKSATGWSQQAKIGSSDGQSNDWFGYDVGVSGEQVLVGAPYADLMGMLD
                     AGAGYTFLRTGAMWTQTAKLTASDVSPSGFFGNGVSVDGETFLVGAPGRPPGGAAYAF
                     IGRKGNGEACLTANDCGSGFCVDGVCCNSACGGGSVDCQACSVAAGAATNGVCGPARG
                     GTICRPAAGGCDQAETCTGMSMACPADVRKPRGSQCRPASHGCDAEEQCDGQNADCPL
                     DSVQKDGTRCTLGSCQLGQCRIESDVAIAWENPTLTVSGFSPGSAQLLLSNKGPSPAY
                     DVAFSVEAPPLSKLALANVPSGWSCTQDLAVLNCLVATLPMGESKAEVTLVPPPKLAA
                     FDLPAVVTSIGTDPDPNNNAATLKVTNDNPMFDQFAGGGRGCAMGGTSHLGTPFAALS
                     MLLALCLVRRRGASNR"
     gene            11733..13022
                     /locus_tag="PKL17_09170"
     CDS             11733..13022
                     /locus_tag="PKL17_09170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK44940.1"
                     /translation="MDCSTCFYPNDDEARFCTRCAAPLGVPVSRLPTEAPAAPHPAIA
                     EMIAGSGDLGTPQGLAEAIQGAGAVPGRGGIGLGGTDLSLLGGRYRLLRCLGSGGFGA
                     VYEAEEVALGLRVAIKLLNRTALQDPKMRQRFLQEARMIAELRSPHVVVLHDYAVTEH
                     GVPYLVMELLAGKPLSHDMLAGVLSPRRAVHIMAQVCGALGDAHAHGMVHRDIKPDNI
                     LLLSHAPPFVQGPSVPPLPATPFGDQDFVKVVDFGVARFCGLSTRASDILGTPAYISP
                     ETLADEPVDGRSDLYAVGMLLWQMIVGDLPFPGKNQALMMKSHLDAPRYRPSEINPRY
                     RLPDGLEDLMVKLVARAPSDRPSSAYAVRAQMHELLSSPGLLSDRPLRGAARPDFGPH
                     WDGPLTDKQMGQRHKPKDPHAATQAITVKIPTRIDAS"
     gene            13056..13973
                     /locus_tag="PKL17_09175"
     CDS             13056..13973
                     /locus_tag="PKL17_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44941.1"
                     /translation="MARLSFVSLPLLGLLLVPLSSAQAAEQWWRDPERGCGKIEDFKR
                     TFRITDLPGCDGELLRSAPEGEVAMHDAKKLLREAEKVLDGGQSDGVESKLNQAIEIM
                     NKAPRDARVDWARQHFKTAVEVLQARMAMLAKLPKLRQSYKALADLSAQAKPDAKAVQ
                     SAAEACVAAFKEAESGGGNLQLQYELVSGSKMKPLRDYMTECEQAKGSSAAATPAAGT
                     GTTDAKPAATDAKPDKPAAAPAGTDGGVPRDKWVKKLKGDRKKVFEAHADAFPLFDGE
                     AGPKGAAKATEWKYGSEVIKFKGNKQLKE"
     gene            13982..14431
                     /locus_tag="PKL17_09180"
     CDS             13982..14431
                     /locus_tag="PKL17_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020862076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNK44942.1"
                     /translation="MPIPQTPLCTVDCIIDLVDHPLHRPDRPVLVLIERRYPPHGWAL
                     PGGFVDIGEPLGHAAVREALEETSLSIELVEQFFSYSAPTRDPRRHTVSTVFVATAQG
                     TPKAADDAGRVAVFTEDNLPDMAFDHGQILADYFAYRRTGKRPGPHR"
     gene            complement(14525..15352)
                     /locus_tag="PKL17_09185"
     CDS             complement(14525..15352)
                     /locus_tag="PKL17_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK44943.1"
                     /translation="MADKTFVTVSFERIQRVVDILSMVSIGEYDPQLIQIEPNPQADE
                     FSVLEAALNIYNQELASARQQNEQYIAEMERSKRELQEQISTIARQQQAIHELSTPIV
                     EIWEDILLLPIVSSLDSQRAAEMTEKLLERVKASRSRCIIIDITGVELVDTNTANAML
                     KMTRAARLLGTMCVLTGIRPEIARTMVQLGVELEGIKTMRSLKDGLRECFAFLHKLKQ
                     QEQQATQSEHGGALGLLQPRRRRDKAAAKAEPTSEDAPSSAAPSPALAPSASSLPGD"
     gene            complement(15468..16319)
                     /gene="mazG"
                     /locus_tag="PKL17_09190"
     CDS             complement(15468..16319)
                     /gene="mazG"
                     /locus_tag="PKL17_09190"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNK44944.1"
                     /translation="MSAPCPAPSSTYVPARGDKLADLLDVMEHLLAEDGCPWDREQTL
                     SSLKNYLIEESFEVLDAIDESDAASHCEELGDVLFQIVFQSALRAREGAFNIDDVCQN
                     IAEKMRRRHPHVFGDRKVKDAAEVVQNWGAIKAEERKTAGKVRRTLDGIPKSMPALKR
                     GQLIADRAAATGFDWPDIAGVRAKIDEELAELDAEVAAGIETASRDRIESEFGDVLFT
                     LTRLAGKLGIDAEQALRRANTRFVGRFEAMEDRITASGQQISQLTLPQMNQHWESVKR
                     SQAEDKR"
     gene            complement(16381..17019)
                     /locus_tag="PKL17_09195"
     CDS             complement(16381..17019)
                     /locus_tag="PKL17_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44945.1"
                     /translation="MNASMIRFAATLLLLTAPFAGGCATTQYAPQLVARGEITLGYDG
                     GFVMRAEGKPVAEGLSYRGLASYVVCVPEAHRHAKSAQSHGGAAIALSVLGVTLSLAS
                     LGGLAGLADTDHQWAWLGGGLASGVVGLTLSALSWREKNQANGHALDAMNLYNDSVGS
                     LGASCQDLRYPQAAGPAAPDGQSLPSTHPTSLSPAPDSLPPPPTVGTPAGPN"
     gene            complement(17016..17747)
                     /locus_tag="PKL17_09200"
     CDS             complement(17016..17747)
                     /locus_tag="PKL17_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44946.1"
                     /translation="MRRLHGLHKRTGRFSVGLLASVVAWLAGLGCGYESSYVPPSDGR
                     ARVIWNSKDSEATVSLSGGGLGRDCQLALRQLTGHARIPVENSFVQLPELESASPYRL
                     SGYGQEYWVPRYYGPDIVVIHPGVPPHLPRPPLFVPAAPRPHVGAIYRGGPSVGGGGR
                     SSGGFRLGGGSGGGGDAGKGLAILAVVAIVVMPVISITLATVRPESEKQAAEAIDAAH
                     ALNDMLRSGGSPCQPEAVPSLGGAP"
     gene            17898..18527
                     /locus_tag="PKL17_09205"
     CDS             17898..18527
                     /locus_tag="PKL17_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44947.1"
                     /translation="MSAALCLGCPNQPEPQAPTVVSKSPTARSPAPDAVTNQLAADLA
                     MVSSLSDAGDAWVAPPSVPSTSPEFVEAQFLDTYRLFVPRDFTSQEKDIRWSRYYQGR
                     WVRWTGQLRFVTADAFLFHHLGPTSSYEVSLQIPEPERSRLRQTLQLGRFYNYVGRLL
                     RYETTFRVIALEQGSVLSPNDIGVPGTLASLPWSHDPPPLPGLVRPGSK"
     gene            18673..19797
                     /locus_tag="PKL17_09210"
     CDS             18673..19797
                     /locus_tag="PKL17_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK44948.1"
                     /translation="MSRDLSGKTLSGYRFGDVLAKGAQSTVYAAYQGDGDRAVAVKVF
                     LSELPKDKTAAGRIVADVERAARVSHKNLVPVIEVGSLEWKGKRHLFVAMERLHGESL
                     KARLQSQPGHSLPLHVALQIASAVGGALQALHGADLVHRGLNPGAVFLSPPTDAERQA
                     DLEAEDHIYLLDAGQSALFEGLNPSSNGKRPKPSDDIRGLAALVSEMLGGVPQTAAQG
                     SEALLPLRFRNRKIPARLDAVLRLVLSSKLGTDKQDPYPSVSAFVAALFGDATNIPTM
                     AAWSSDGRAMVQPTRNRSGLMWAAILSVVGGASFGVGYWLYQEPPQTPKADPAPVISV
                     PPPAAEALDMAKSAAAPSANSANPSTGPAAATAPAKSEVK"
     gene            19819..20400
                     /locus_tag="PKL17_09215"
     CDS             19819..20400
                     /locus_tag="PKL17_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK44949.1"
                     /translation="MVLLAGLLPGLLSTAPALAQQPAAPAAAAKPTTPAAPAAAAKPP
                     VAINPQTAALAEKAIGLAQKDQYEEALKLFRDAYRIDRNPRWLYNVGVLYDRMANCDE
                     AAFFYRAALFGQGVLPQDVDLVDNRLGVLQEECNFRERHKQVADRHARATRYMSPEFR
                     LCALAESILTGIMTPQERQQLATCKATENTPSP"
     gene            20476..22119
                     /locus_tag="PKL17_09220"
     CDS             20476..22119
                     /locus_tag="PKL17_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44950.1"
                     /translation="MKRPALASRIFRTAGSLALLAASLAPATSSGYAIQIHNELARRA
                     LLKSNLQDAASPISPAAFDQMRTIIDSFARNEPTLRDAWQKRYPTAANFDAFAEKQLL
                     QLAASSKVFGIDAVDERLKAGATLLDVVSWGAGQPDDDWRNRERLAYDDKRQPLKDKY
                     GQPVPADPALLNMGRLGALSSQAHAHYGLAQVEFSGDADVLKKEPKRFARAVGWEKGP
                     IITLAADMAQIHLDMSLACALSDVPTARELSWQFTGEGFHYLGDVGNQIHTVQVGLYD
                     FFVDAALERLKMGLLSGGGYLGKMRSLGSIGIDILSSHHTLSEEFTYRRFKAAVEGKA
                     TPETERLLSAPTEDDPEFAKKLDEALAKLGPNPERGEFAMVLTRTMIDVSSDQGDDVY
                     RATAGIGDPALRSRHKTFDEEHDDPEKFVRPRDASTESAYKEFWSLQEQAFRRVGTAK
                     RRWVQLQQKLLASATTPEQKEALRKEVLKRLLDRQLKMLDASDARLAQYLADPPQNIS
                     APEKAPELLGADVVIIGLLVGIPLIIVRRRQKAQSKPQA"
     gene            complement(22573..22878)
                     /locus_tag="PKL17_09225"
     CDS             complement(22573..22878)
                     /locus_tag="PKL17_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNK44951.1"
                     /translation="MRVEFTSKAAAQYRLAFDRIAEVNRFASQRFAARLHARLRQLGR
                     FPRSGHYIAEFATLPVRQFIVEPYRFFYEIDERNNVVRIVSVWHGAQLPAQPDLPAP"
     gene            complement(22875..>23083)
                     /locus_tag="PKL17_09230"
     CDS             complement(22875..>23083)
                     /locus_tag="PKL17_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44952.1"
                     /translation="VGSQRGRTQPGDGEMMDPVPPPLPSDPAERAALFASIEEGIADG
                     RAGRVIAWEVLDRECRQKHGLPPA"
BASE COUNT         4562 a         7190 c         6706 g         4625 t
ORIGIN      
        1 accacgctgg atttgccggc aacggtcagg cgcgattctc tgctttccgt cgatgctggc
       61 atcaccaaga ccctgcccgc gggtttcagt atcgacctga cggtcacttt catgcgaaac
      121 gtgtcgaaca tcgccaacgg aatcgacaac cgcaactact cgaagctgac caccatgctg
      181 ggtatatatt acagctttta aatgattcat tatcatgcgc gctgatccca ccctgcttgc
      241 ggacgatctc tgtgaactgc ttcatcgtac aagcggattc ccagaagatc agaccgtggc
      301 caagctgttg tgcgcaacgc ccgatgctgt tgacggtgcg ctcgctgaag tcgcaagccg
      361 cggtctgctt ccatccttgg tttctacgat gctgcgtgct gaagatgacg ccctccttgg
      421 cgcagtcacc atgtttgcca agtcgccggg cgttcgcgtc gcgctctgtc acgaagtttt
      481 gcaagtcgaa gaccaagatg tgcaggccgc gctggaacgg ctgctgattt ccgcgaccga
      541 tcagcttccc accgtgtttt cgcagcttgt cgatcaaggt gattacgaag tcctgctgaa
      601 cgcgctgcgg gtgacagagt ctgccgatac accgacgctg cgggcgctgg ctgcggcacg
      661 accgacttac ggaatggatc agcttaacgc aagcgctttg catcgcctga cctcctctgc
      721 gaaacagact ccgtatgatc tgctgattgt gccgggcttt acgccggtca acgccaagca
      781 gccgattcac ctgcgcgatc tgcctcccgc gcaggcgcgc attcagctcg cggcgcagga
      841 tctgaaaagc ggtctggccc gtgcggtttt ggtgtccgga ggttcggtgc atccaccagg
      901 aacgccgtat aacgaagcgc tgatgatgcg aagcggtctg ctggaattgg gaatcccagc
      961 cgaacagatt ttggtcgatc cacatgcgcg ccactcgacg acaaacttgc gcaatgctgg
     1021 tcgcgccatg ctgtcactgg gaatgggtcg cggactgatt gtgacaggct ttgacagtgc
     1081 gccgttttcg caggcgttct actttgcgca tgcgaacctt tcgacgttca acctgcgctg
     1141 tcagcatgag ctgggctatt cggtgggaac gctcaatgaa cgcgatgcgc atcatgtgga
     1201 gttttcgcca agcggcgatg tcacgcggaa aaactacgcc gatcccctgg atgcgtagac
     1261 actagcgcca cagtgcaatt ccaccgagca gtcccgcgat gttgtcgatt ttgacgacac
     1321 cgtccggcag cttgccttcg tacttggcgg cattcccacc cccgacatac agcttgtctg
     1381 ggttgaatgt cttttgaatc tgcttgatca ccttgcgaac ctgcgcttcc cacttgcgct
     1441 tgccaagccg cttgcgcgcg cggttgccga cgttttcttc atacgtccgc tgtttggtca
     1501 ggggatggtg cgcaagctcg acattgggaa cgtagtgccc gtcgatgtac agcgcaaaac
     1561 ccatcccggt tcccagcgtc accacgatct ctgttccgtg tccttcaatc acaccgagcc
     1621 cttgcaggcc cgcatcgttt aggacagaga ccggcttttc cgtcatcttc gcaagcgcac
     1681 tttgaagatc aaatccgacg aagcttgcgt ccagattggg ggccgcgaaa atcactccct
     1741 ttttcaccac ccctggaaaa ccaaccgaca ctcgatcgaa tgtcgtatcg ggaagcagac
     1801 tccgaatcac ggccaagacc gcgtccgggg tcgctggatt gggtgtctct ttgcgctggc
     1861 gcttgccgct tggctgtcct gcggaatcaa gcagcagaca cttgatcccc gttccgccaa
     1921 tgtcaatcgc cagcgtgtag ggaggactcg gcgcatcggt cgaagtcgag ctcggcgctc
     1981 cggtctcttc gcgtacatcg tctaagggct tcccaaaagg cgcatcagag acggtggtca
     2041 tcccttcttt ttatcacggc acatggcgca gacacaagga ccgcgtgcgc ggctttcgga
     2101 atagacggag cctcggtttt cgcgtaccct cttttgcagt atgcgtgccg gattttggtc
     2161 gtctatgctc cctgcgttcc ctacgctgcg tcgaaagcta gacctttttc cttgcctttc
     2221 gctggcgctg ttggcgctgg ccggcggctg tgcaaaaccc gaactggtcc gcagtcctac
     2281 ccaggtggtt tcgtatcttg ccgacggtga tgcgcagctc gtgcagtctg ttccgcaagg
     2341 gacagagctg ctcgatccga cgctgccgac aacgcagctt gtctggatcg aaatgatccg
     2401 cagcgccaaa cggacgattg actgggcctg tttttatgtc gcctcttcac gcggccaagc
     2461 gctcgatccg gtgctcagtg aactttcgca agctgctgcg cgtggtgtgc gggtacggat
     2521 gatttttgac aagcagatgg cgaagacgga cccgcaagcg ctggcgtttc ttttgcggat
     2581 gccaggggcg gccattcggg ttctggactt caacctgatt gcgaccggaa gtcaccatgc
     2641 aaagtatctg gtggtcgacg ggcgcgagct cttccttggc agtcagaatt ttgactggcg
     2701 ttcgctgtca caaatccatg aattgggagt ccggctcgcg caaccacaca tcgtgggaca
     2761 gctcgcattt ttgttcgaaa tggactttgc actggcgcag gatcgcaacg acacactcga
     2821 atcgaatccg cctgtgttgc cgcgcgggcc gactccggaa atcgaagtag gaatttcgcc
     2881 accgcagctt acacccgacg gaatccgtcc tgcgctgccg gtgctactcg acatgattcg
     2941 tggcgcaaaa tccacactgt cgatacagct tctgcgctat tcagcccaca gtggcaccga
     3001 tcattgggac acgttagagg aagcgctgct catggcggca tcgcgcggtg tgaaagttca
     3061 gcttctcgtc tcgaactgga gcattcagtc acccgaaatc gatgatctaa aaaagctgtc
     3121 gcaggttccc aacatcgaag tccgcattgc cagcattccg gaacttcccg gccagttcat
     3181 tccctatgca cgggtgattc acagcaagta tgccgtcgca gatgaacatc agttgtggct
     3241 gggaacgacc aactgggaac gcggaatgtt catggccagt cgcaacgtcg acgcggtgtt
     3301 ccgcagaccc gtgctggcca aacaagcgca gcagattttc aatcggctgt ggaagtcgtc
     3361 ctattcggcc ccggttgatc caagcaaaga ctacgtgcca ccgcgggtgc gctaagcgcg
     3421 gtccacatta cggggccaga tacattccgt tcgagctggc acagctctgt ttgatggaat
     3481 ctgcgcttgg atagtagccg ccgatgtaat accaactgat ctgcgtcacc gtcgacaatc
     3541 cgacattgct ggtcgtctta cagcccccga gtgcgccggt ccgattgcag ccgaccgtac
     3601 tccagcttcc gccagaagat gcacacgaca tcttgtagga agtcaggatt gtggcatcgg
     3661 taacaaactc ctgacagtac agcatgttgg ccttcatcgt gggtcgcaga tcgcagctct
     3721 gtgcgtaggc ggtcattgtg gtgttcgtgg cgtcgaccgc tgcgtcggta gcaccggtcg
     3781 atgttccatc gcaaccagcg tgggacagtg ccaaaaggat gccgaaacag agtcctgctc
     3841 ttatatgaat catttgatac tcctcttatc tgcgatgttc agttttcgct tttcggaccg
     3901 attggcagat agattccgtt ttccattccc gcccgcatca gcgctttcca ggtaaagaac
     3961 tcaccacggc gcacgctcag cccgtgtacc tgcgatccga cgagcgagcg accgcttgga
     4021 cttctggctg gaaaacgaaa tacggttttt cctttcggaa acgatcgctg caagctggcc
     4081 atcggctgcc ggtggtgatc gagcagtgtg atggtcacgt cagatacggg cgcttccgtt
     4141 tcgaccgcaa tgtctggtga cgcatcggga tcatagctta tcagcaggct gggaatgtcc
     4201 gtcggtgcgg ccactgtata actctgcgcc caggggtctt tgcgcgccgt ttccggaagc
     4261 tctcctgatg ccagatcatc cagccggctg gcggtgccac ccattggctg atgggtttca
     4321 taggcgcgcg caatcggtcc tgtcatgatt cgtggaccac cattagtatc gacgacaaac
     4381 agccccaaac ggacatcggc aatgccgaca tacgacgctt cgcccaagtt ggtggacgtg
     4441 taatagtcgg cgacaagctg gctgcgcccg agtccgtccg ccgctcgctt cctaaacaga
     4501 tcaaaatacc atccggtgaa cgtgggtgga cctccggatg aaccaggcgc catttccacc
     4561 accatcgaca ggaaacgttt ttggttcgct gtcagcgtcc ttccggccag ttcatcatcg
     4621 acaatcaccg ataagatggc gagcagtcga tcgagtcgct gaaaatagga acgcgccgca
     4681 gtcttgtctt ctggatcaag tgccgtcgct gcttgcacgc cgcggcgggc atactcacgc
     4741 agaccgcgca gtgtttcagg ggccggctca acatagccat cgggaatctc gcacccgccc
     4801 agatcatacg gctgtcccgc gaccagaacg tagttgtgtt tgatctgggc atagccggct
     4861 gccgtcgagt tgatccgcag atcggcatac gcatcgctgt ccataaacga tggatgcacg
     4921 ccgcttggtt tggccgcgag tgtttccaca gaacccagcc acgctgaata cagatcggtg
     4981 cgtggctgcg caggggaggg cacagacacc atcgtccgtg acagatccaa gttctttttg
     5041 agcgacggaa acttcgcaag ttcaggggtc aaataggaca gcgcccgatc gtgcccgagc
     5101 gcataggcga cctcggaaaa atgcagccga tatttgtcag gaaccgcatc atgaaccagc
     5161 ggacgcaggc gctgtgcatc tgacaccaca cgggggccca gcaccgtcgc aatcaccggc
     5221 agattcaccg atccctgcgg catgtagtgc atagacactg tgcggccaaa acgattccca
     5281 atcgcagtcc ggagcttatc ggacagcgac tccgcacgca gatcggtgat gcccgcttgt
     5341 ttttgcagca ccagcagttc cggtaacgag acatcttccc ggcgacctgc caaaaacgac
     5401 cacagctcgt cgagcgcccg cacttccgct gtgacgttgg cttgctgcat caagttggcc
     5461 agtgccagcg ccagtgccac ttcacggggg gtctcgcgtg gatcagggga cgtgcccggt
     5521 gccgagctgc gcgacgatcg tgacaccagg ttgaattcca gccgtgacag ccacatcgcc
     5581 gtttgaaaca gcgcagtcat ttgcggttct gccgcatagt gaccacgcgg tgcatactgc
     5641 gaaaaatcga tcatccgcgg gcggccaaac aaagacacgc cggtcaaatc tttggccgat
     5701 cgaatctggg caagcagact gctcgcttcg ttttctgtca cctgaaaaag cggcggatgt
     5761 ttgtccccca gaagccgcgc cgctaccgtc aaatacagat ccagatcgtg cgcggtgtcg
     5821 gcagaaatcg cctgctgaaa actgggaagc ttggcgcgcg tttccgtcag aaatttgatg
     5881 atgttgggaa ccgcgatccg ctcttccaga tccgccatca agccgtcgtt tgacgcatag
     5941 accgcatgca gaagcgcatc tatcgaaaca tacagcggca gctgggactg atagatttca
     6001 tgcagcgccg tcacgtagct ttcgaacgac agacgagcca gcgataccat tccctgtttg
     6061 taaaggagtg cgcgttcgga cggacgcagc gtgaagcggc gctcaaactg atccagatac
     6121 ttgggggtcg tccgcttgtc ccagcgttgg ctagcagcga cggcaactgg acgctgcgca
     6181 tcacgcagga tcgcatcgcc cgccttctgc aagttggaat cggccacctc gcaggcattt
     6241 tgcggattgc gcggtacttt ccgcctgtcg ccatactctt cttcgctgcg gactccctgc
     6301 gatgccaaca gcaaccagac agaaatccca catcccagcg tgcgtgcgga aagactctgc
     6361 gtcacgtttt gccatccccc caagcgtccg agtatagcgg aagccgtggt aaggtatcgt
     6421 ccgaaagatg tcagtcgcac aaactgcaaa aattccgctt cgtcacgatg tatgtgatgc
     6481 gtatgaatgt ctgtctggcg atgccgattc gccgcttctg ctcacctgtg aacatgcgag
     6541 tcagcgattc ccaagtggct atgcgctgca agcgcgcgac aaccggcttg tcgataccca
     6601 ctgggcgttc gacttgggtg cgcgcgagct gacgcatgaa ctggcttcgg ccttgtctgc
     6661 gacggcggta ctcgccaatt tttcccgtct actgattgat ccaaaccgcg aggaacacca
     6721 tcccgacctg ctgcgcacga ccgcagatgg tgaagcgatt ctgctcaatc agaatgtgac
     6781 cgcttcggat cggctgcacc ggaccgtgtc ctattaccga ccgtatcacg aagcggtcga
     6841 cgccgcgctg gcccgatcga atgcgagtgt gctgctgtcc attcatacgt tcactccgat
     6901 ctatgaagga cagccgcgga cggtagaact gggggtgttg tttgatcgcg atgagagtca
     6961 ggcagagtca ctgttgtcgt cgctgcgcgc tgtctttccg cacgttgcag ccaaccagcc
     7021 gtggtcagga aagttgggac ttatctattc agcggaatca cacgcccatc ggtttggccg
     7081 gaccgctctg gagctggaag tccgtcaaga tcgcgcgcaa gatcccagct atcggcagcg
     7141 actgattgcc gtgctgtgcg accactttcg ccattcttca atatgaagca tggaaaatga
     7201 ttcatattgg atttggtcca atatctgtcg acgctgcgct acggatacag ggtttctggt
     7261 ccggcgcagt tttcatccag caccagcgtg gtgtgtacct gatgagatgg aaagtcgctg
     7321 acgcggacaa gaccggcggg ctcagcgggt tttccgggca tacaaagcac cccatccccg
     7381 tcggtatcta gccagccgta gaccaccaga ccttcgcctt tttgttccgg atacagcagc
     7441 gtttcatcaa aacggtaggg caaattttcc agcgtcactt tgcgcgaatc gaagagccca
     7501 agcggatgtg acagcgcgcc actgccgaac gtcttggcgt gatgaaattc caggtgaagc
     7561 gtgcccttgc ccgtcgctga aacgcttccc atcacctggg tttcgtacag cctgatgtca
     7621 tcatttacac aacccaaaag cagagcggcc agaatcatcc gcttcatcgt ccgcctccga
     7681 aatgcagtcc cacatgcgcg cccggaatcg ctacgcttcc tggtgcatcg acccagccat
     7741 agtcaatcgc gacatcgacc ccaagcgacg tcgtggcgcg aattcccaga cccaacgttc
     7801 cccacggcgt ccattcgtgt cgcaccgtcc acgtcgtgtc tacacccgat tcccgttcaa
     7861 tctgggtgcg gctgggaagg atggcatgtc gggttcctaa caccagataa ggaatcagcc
     7921 ggcccaccga aaatgccgcc cgcagacgca gctcgccgct tggtcctcgt cgcgcaagct
     7981 ctccttcttg aaacatccag ccgagcagca cttcgccaga caaacggaaa tgcggacggt
     8041 tcacccacgc caaagacagt ccgagcgacg tggtaaatcg ccgacccagc gccgtttcca
     8101 gctcgaacag cggcgcaagc cgatggccca cgccatacat cgtgcgcagc gaaaaccacg
     8161 gatagcccgc ctgcaccgac acgccccaca ttcctttttg cggacccccg gcaagctgat
     8221 caagcaccac gggatctggc gcaagcagca gcgtgatcac tggttgggat gtctgttcgc
     8281 tgggattttc cgtcggcggt ggctgatttg tttcggcctg tagcgcaagc agcgcaagga
     8341 cagcagtcgt caacatgatg cgctcacttt gctttcgcga cgccaaacac gcgcgcaaaa
     8401 ttttccgaaa acaccgcggc ggcttcgtcg gcggtatagc cgcttgcttt catcgctgtg
     8461 actgtgcgcg acagatagtc tttgacaaat ccagggctct gcggaccgtc gctgccgtaa
     8521 atgatccgat caacaagacc ggcatcgcgc agcttctgca tcgcatactt taggttttct
     8581 cccgtcggat ctgacccctt cgatccaagc gcgctaggtt ccagatacac gttgggatat
     8641 gtcttcgcca gccggatgca cgcatcgagc gacccaggct gctgatcacc aaagtcatat
     8701 cccagatgtc cgaggataaa caccgcaccg ggatagcttt ggatcgccgc ttccagatac
     8761 gtcggatcgg tgtacggcgg ctcgcttgat gtgcccggaa acggactcga ccccgtatgc
     8821 aggtaaagcg gttttttcag atccgacgcg acctgataga tcccaaaaaa cgtcggatca
     8881 tccattcgga agtgctggtg tgcatgcgcg agcttgatcc caatcatcga cggattggac
     8941 agcgccgcgc gcagccgatt tagctctgcg tctttgtcgg tacggaaatt gtcaacgcgc
     9001 agactggcca gtccgtacag cctatctggg gcctttgcgg tatcggacag aacccgttca
     9061 ttggtcgcga ttccgacggt gcgtggtgaa tacactgcga acagaaccgc ttgggaaacg
     9121 ccagctttgt ccagctcggc caggattcct tccgctgaca gaatgccttc tgccgtcgat
     9181 ttggggttca agttcagcgg aaacggaaag cgactgccga gaaacgtgcg cgtggtttcc
     9241 ggaatcgcat cccattcccc tggatgcaag tgcatgtcga ccaccggcaa gcggcgtcca
     9301 tcccaatcga cgccccgcgg tgtgtcacag cccagcgcca gcagcgccac gcccacaagc
     9361 agacagctac gcagtcgacg catcatcggt tcgatgctcc ccgccggcga accaggcaca
     9421 acgccagcag catagacagc gcggcgaatg gtgttcccaa gtggcttgtg ccgcccatcg
     9481 cacagccccg acccccgccg gcaaactgat cgaacatcgg attgtcattt gtgaccttca
     9541 gcgtggccgc gttgttgttc ggatcggggt ctgttccgat gctggtcaca accgcaggca
     9601 gatcgaaggc ggccagcttg ggcggtggca caagcgtcac ttcggctttg ctttcgccca
     9661 tcggcagcgt cgccaccaga cagtttagga cggccagatc ctgcgtgcag gaccagccgc
     9721 tcgggacgtt tgccaatgcg agcttgctaa gcggcggagc ttcgacggaa aacgccacgt
     9781 cgtaggctgg gcttgggccc ttgttcgaca agaggagctg ggcagaaccg ggggaaaatc
     9841 cgctgacggt cagcgtcggg ttttcccatg caatcgccac atccgattcg atccgacact
     9901 ggccaagctg gcagcttccg agcgtgcagc gggtgccgtc tttttggact gaatccaaag
     9961 gacaatcggc attctgtccg tcgcactgtt cttcggcatc acagccgtga ctggccggac
    10021 gacactggct gccacgcggc ttgcgcacat ccgcaggaca cgccatcgac atgccggtgc
    10081 aggtttcggc ctgatcacag ccgccggctg ccggacgaca gatggtgcca ccgcgcgctg
    10141 gaccgcagac cccgtttgtg gccgcgcccg ccgcgacgga acacgcctga cagtcgacgc
    10201 tgcccccacc acaggcgctg ttgcagcaga caccgtcgac gcaaaagccg ctgccgcagt
    10261 cgttggcggt gaggcaggct tcgccgttgc ctttgcgccc gatgaaagca tacgccgcac
    10321 cacccggagg ccggcctggc gccccgacca ggaaggtctc gccatcgacg gaaacgccat
    10381 tgccaaaaaa gccgcttggt gacacatccg acgcggtgag cttggcggtc tgcgtccaca
    10441 tcgcgccggt ccgcaggaac gtgtatccgg cccccgcatc gagcattccc atcagatcgg
    10501 catacggcgc accaacgagg acttgttcac cgctcacacc gacgtcgtag ccaaaccaat
    10561 cattgctttg tccgtcgctc gatccaatct tggcttgctg gctccagccg gttgcgcttt
    10621 tggtgaacac ataggcgctg ccgctgttga ccttgccgcc cgaatcgccg tacagcgcgc
    10681 caaccaccgc agttgtcccg tcgaccgata cctgccggcc aaaccagtca ccgctctgtc
    10741 cgtctgcggc cagcagcttg gcgctctgcg aaaaacccat cggaccgcgc tggaaaacgt
    10801 aggccgcgcc ggcttcgctc ttgccgcttg gatcggcgaa gtcggcaccg atgaccaagg
    10861 tgtcgcccga aagcgccaca ccgacgccaa acgaatcacc cgcttgggcg tcgctggctg
    10921 tcagcttgcc aaacgccgcc cagttcggtc cgcgcttgcc gaacacatac gcagcaccgg
    10981 cgtctttgcg catcatgacg tcggcaccgc gcgcccccac cactgcgata tccccgtcga
    11041 gcgcaaccgc aaaaccaaac tgatcgtcgc tcccaccgtc gaacgccgtc agcttggctt
    11101 gttgattcca cgtcgttccg gtgcgcgcaa atacgtatgc ggccccgaca tcggtgcggc
    11161 caaactgatc cgcccccggt gcgcccacga cgagcgtgtc gcctcgcagc gccactttgg
    11221 caccaaaatt gtcaccattt cgaccgtcag ccgcgacgat tttggcctgc tgcgtccaag
    11281 tggtgccgtt gcgaacaaaa atgtagaccg cgcccgcatc ggtacgaccc atcggcgccg
    11341 cgtagggggc cgccactacg gcagtgtctc cgtcgatgga taccgacgcg ccttcgtaat
    11401 cgccggtcgc tgcatccgat ccgaccaggc gctgcgccgc cgaaaccgtg accagcgtcg
    11461 ccgtgtctgg actcgaaaac atcggctggg tcggctgcac cacgtcgcga agcgcgcgca
    11521 gcggacccac atccgtcgaa ccggcatggg cgctcgcggg cagaaaggaa aatgacagcg
    11581 agcacagtgc aagcgccttg gctccgtctg aaaccagagg caatgccgag agaacccagg
    11641 tgtaggcctt catgcgcctg tgtgtagcac aatgcggaca aaaattgccg accaccggag
    11701 gcccgataaa gtcaaagtca ccggtttgtg acatggactg ctcaacctgt ttttatccga
    11761 acgacgacga ggcacgcttt tgcacccgct gcgccgcgcc gctcggggtg ccggtttcgc
    11821 gactgccgac cgaagccccc gccgcgccac atcctgccat cgcagaaatg atcgcgggca
    11881 gcggcgatct gggcaccccg caaggactgg ctgaagcgat tcaaggcgcg ggcgcagtgc
    11941 cagggcgcgg tggaatcggg cttggcggaa ccgatctgtc gctgctggga ggtcgctatc
    12001 ggctgctgcg ctgtcttggc tctggcggtt ttggggcggt ctacgaagca gaagaggtcg
    12061 cgctcgggct tcgcgttgcc attaagctgc taaaccggac ggcgctgcaa gatccgaaaa
    12121 tgcggcagcg gtttttgcaa gaagcgcgca tgatcgccga gctgcgcagc ccacacgtcg
    12181 tggtgctgca cgactacgca gtcaccgaac acggcgtgcc ctatttggtg atggaactat
    12241 tggcagggaa gccgctgtca catgacatgc tggctggcgt gctttcaccg cgtcgagccg
    12301 tacacatcat ggcccaagtc tgcggcgcgc ttggggatgc ccatgcccat gggatggttc
    12361 atcgcgacat caagccggac aacatcttgc tgctttcgca cgcaccgcca tttgtccagg
    12421 gcccgtctgt accgccgctt cctgcgaccc catttggcga tcaggacttt gtcaaagtgg
    12481 tcgattttgg cgtcgctcgc ttttgtggtc tgtccacacg cgcttcggac attttgggaa
    12541 cgccggccta tatctcgccc gaaacgctgg ctgatgaacc tgtcgacggt cgcagcgatc
    12601 tgtacgcggt cggcatgctg ctttggcaaa tgatcgtggg cgatctgccg ttccccggaa
    12661 aaaaccaagc gctgatgatg aagtcgcacc ttgacgcgcc gcgttatcgg ccttctgaaa
    12721 tcaatccgcg ctaccggttg cccgacggac tggaagacct gatggtgaaa ctggtggcgc
    12781 gcgcgccaag cgatcggcca agcagtgcct atgcagttcg cgcgcagatg catgagcttt
    12841 tgtcgtcgcc agggctcttg tccgatcgcc cactgcgcgg tgccgctcgt cccgattttg
    12901 gtccgcactg ggacggtccg cttacggaca aacaaatggg acagcggcac aaacccaaag
    12961 acccacatgc tgcgacacag gcaattaccg tgaagatacc gacgcgaatc gacgcctctt
    13021 gaccgaagct actaactctg ctgagttaat aagctatggc caggctttcc tttgtgtccc
    13081 tgcccctgct ggggcttctc cttgttccgc tctcctccgc ccaagccgcc gagcagtggt
    13141 ggcgtgatcc ggagcgtgga tgcggcaaaa tcgaagactt taagcgcacc ttccgcatca
    13201 ccgatctgcc gggttgtgac ggtgaactgc ttcgctcagc gccggaaggc gaagtggcga
    13261 tgcacgatgc gaaaaagctg ctccgcgagg ctgaaaaagt cctcgatggt gggcagtccg
    13321 acggtgtcga atccaagctc aatcaagcga ttgagatcat gaacaaggcg ccgcgcgatg
    13381 ctcgcgtcga ctgggcccgc cagcacttca agaccgcggt cgaagtgctt caggctcgga
    13441 tggcgatgct cgcgaagctg ccgaagctgc gtcagagcta caaagcgctg gccgatctga
    13501 gcgcgcaggc caagcctgac gccaaagcgg tgcagtcggc agccgaagcc tgtgtcgcgg
    13561 cgttcaaaga agcggagtct ggtggcggca atctccagct tcaatatgaa ctggtgtcgg
    13621 gcagcaagat gaagccgctg cgcgactaca tgaccgagtg cgagcaagcc aaaggcagca
    13681 gcgctgcagc gacgccagcg gctggcaccg ggacgaccga cgcgaaaccg gctgcgaccg
    13741 atgccaagcc agacaagccc gcagcggctc cggctggtac ggatggcggc gtgccgcgtg
    13801 acaagtgggt caaaaagctc aaaggcgatc gcaagaaagt ctttgaagcg cacgccgatg
    13861 ccttcccgct gttcgatggc gaggctggtc ccaagggcgc cgccaaggcc accgagtgga
    13921 agtacggcag cgaagtcatc aagttcaagg gaaacaagca gctcaaggaa taggcgccga
    13981 catgccgatt cctcaaacgc cgctctgcac ggtcgattgc atcatcgatt tggtcgacca
    14041 tccgctgcat cggcccgatc ggcccgttct ggtgctgatc gagcggcgct atcctccgca
    14101 cggctgggcg ctgcccggtg gtttcgtcga catcggcgaa ccacttgggc acgctgcggt
    14161 acgcgaagcg ctcgaagaga cgtcgctttc catcgaactt gtcgagcagt ttttcagcta
    14221 ttcagccccc acgcgcgatc cacgccgaca taccgtttcc acggtgtttg tcgcgaccgc
    14281 gcaggggacg ccgaaagccg ctgatgatgc cggtcgggtc gcggtcttta ccgaagacaa
    14341 cctgcccgac atggcgttcg accacggtca gattttggcc gattatttcg cgtatcgtcg
    14401 caccggcaag cgtcccggcc cgcaccgcta acccgacatt tctgcgcaaa atggctcgcg
    14461 atggcgctgc atcacgcggc gcgactgtcg cggtgcgttg tctgttttgt gcgatgtcgg
    14521 tcggttagtc gccaggcagg ctcgatgcgc tgggggcgag ggcaggggac ggggcggcac
    14581 tggatggcgc gtcttcgctt gtcggttccg cttttgctgc ggctttgtcg cgtctgcgcc
    14641 tcggctggag cagaccgagc gcaccgccat gttccgactg cgtcgcttgc tgttcttgct
    14701 gcttgagctt gtgcaaaaac gcaaaacact cgcgcagacc gtctttgagc gagcgcatcg
    14761 tcttgatgcc ttccagctcg acgccaagct ggaccatcgt gcgggcaatt tcgggccgga
    14821 ttccggttag cacgcacatc gttccgagca gccgcgccgc ccgcgtcatt ttcagcatcg
    14881 cattggcggt gttggtatcg accagctcga cgcccgtgat gtcgatgatg atgcagcgcg
    14941 atcgactggc tttgacccgt tcgagcagct tttcggtcat ctcggcggca cgctgcgaat
    15001 cgagcgagct tacgatcggc agaagcagga tgtcttccca gatttcgacg atgggcgtcg
    15061 agagctcatg aatcgcttgc tgctgacgcg caatcgtcga gatttgctct tgcagctccc
    15121 gtttcgaacg ctccatttcg gcgatgtact gctcgttctg ctgccgcgcc gatgccagtt
    15181 cctggttgta gatatttagc gcagcctcga gcaccgaaaa ctcgtcggcc tgcggattcg
    15241 gttcgatctg gatgagctgc gggtcgtatt ccccaatcga caccatcgac agaatgtcga
    15301 cgacccgctg gatgcgctcg aaggagacag tgacgaaggt cttgtcggcc atgacacgca
    15361 atatgtgcgc ggaaattctg tacctaagcg cacggaatta tcttcattcg ccaggcatga
    15421 tcaagcaagc cttttcgccg accccgccac acttttccac cgtcccttca gcgcttgtct
    15481 tcggcctgcg atcgcttgac cgactcccag tgctggttca tctgcggcag cgtcagctgc
    15541 gaaatttgct gtccagatgc ggtgatgcgg tcttccatcg cttcaaaccg accgacaaag
    15601 cgggtgttcg ctcgccgcag cgcttgttct gcatcaatgc ccagcttccc cgcgagccgc
    15661 gtcagcgtaa acagcacgtc cccaaactcg gactcgatgc gatcacggct ggctgtttcg
    15721 attcccgcgg cgacttcggc gtccagctcg gccagctctt cgtcgatttt ggcgcgcact
    15781 ccggcgatat ccggccagtc aaaacccgtc gctgccgctc tgtctgcaat cagttgaccg
    15841 cgcttgagcg ctggcatcga cttgggaatc ccatccagcg tccgccgcac cttgcccgcc
    15901 gtcttgcgtt cctcggcttt gatcgcgccc cagttttgta cgacttccgc tgcatctttg
    15961 acctttctat cgccaaagac gtgcggatga cggcgccgca ttttttcagc gatgttctgg
    16021 cacacgtcgt cgatgttgaa ggcaccttcg cgggcgcgca gtgccgattg gaagacgatc
    16081 tgaaacagca catcgcccag ctcctcgcag tgcgacgctg cgtcgctttc atcgatggcg
    16141 tcgagcacct cgaacgactc ttcaatcaga taattcttca ggctcgacag cgtctgttcc
    16201 cgatcccacg gacagccgtc ttctgcgagc agatgctcca tcacgtcgag aagatcggcg
    16261 agcttgtcac cgcgggccgg aacatacgtc gaagaagggg cagggcaggg cgcagacatg
    16321 ttttgggaac ctcgctgccc gcatgtctag cgcagcgcgg cccggtttgt catccccgtc
    16381 tcagtttggg ccggctggcg ttccaactgt cggcggcggc ggcaagctgt caggagccgg
    16441 cgaaagcgac gtcggatggg tcgatggaag gctctgtccg tccggggcgg ctgggcccgc
    16501 ggcttgggga tagcgcaggt cttggcagct tgctcccaga ctgccgacgg aatcattata
    16561 caggttcatc gcgtcgagcg cgtgaccgtt ggcttggttc ttttctcgcc acgacagcgc
    16621 cgacagcgta agccccacga cgccagatgc cagcccaccg ccgagccatg cccactggtg
    16681 gtcggtgtcc gccagaccgg ccagcccacc cagcgatgcc agcgacagcg tgacgcccag
    16741 caccgatagc gcaattgccg cgccgccgtg gctctgtgcc gacttcgcat gccggtgcgc
    16801 ttcggggacg cagacgacat agctcgcgag tccgcggtag gatagacctt cggcaaccgg
    16861 cttgccttcg gcacgcatca cgaaaccgcc atcatatccg agcgtgattt caccgcgtgc
    16921 gacaagctgg ggcgcatact gtgtcgtcgc acagccgccc gcaaatggcg ctgtcaaaag
    16981 cagcaaagtc gccgcaaaac gaatcatgct ggcgttcatg gcgcacctcc caggctcggg
    17041 acggcttcgg gctgacacgg cgaacccccg ctgcgcagca tgtcatttag cgcatgggcc
    17101 gcatcaatgg cttccgctgc ctgcttttcg ctttccgggc gtaccgtcgc gagcgtgatc
    17161 gaaatcaccg gcatgacgac gatggccacc accgccaaga tcgcaaggcc tttgcctgcg
    17221 tcgccaccgc cacccgaacc tccgcccaga cgaaaaccac cgcttgatct gccgccgcca
    17281 ccaacggacg gaccgccacg gtaaatcgca cccacatgcg gacgcggcgc agccggaaca
    17341 aacagcggtg gacgcggcaa atgcggcggc acccctggat gaatgaccac gatgtcgggc
    17401 ccgtaatagc gcggcaccca gtattcctgg ccgtaacccg acagccgata tggcgacgcc
    17461 gattccagct ccggcagctg gacgaagctg ttttcgactg gtattcgcgc atggccggtg
    17521 agctgacgca gcgcaagctg acagtcgcgc ccaagtccgc cgccagacag tgacacggtt
    17581 gcttcgctgt ctttgctgtt ccaaatgacg cgcgctcggc catcgcttgg tggcacgtag
    17641 ctcgattcgt agccgcagcc cagaccggcc agccaggcga caacacttgc cagcagaccg
    17701 accgaaaacc ggcccgtgcg cttgtgcaaa ccatgcaatc gacgcatatg cccacggtag
    17761 ccctgggtct gcacagtggt caagcgccga taatgtatat tatgtcaact aaacatccca
    17821 gcgactttac ataggctttg ccgtgggcag atactggctg ggtgtcgtct ttctgtgctg
    17881 cggcgcgatg gcttgtgatg tcggctgccc tttgtctggg ctgtccgaat cagcctgagc
    17941 cacaggcccc gaccgtcgtg tcgaaaagcc ccacggcacg ttcccctgcc ccggacgccg
    18001 tcacaaacca gcttgcggct gacctggcca tggtgtcgtc tttgtccgat gctggtgacg
    18061 cctgggttgc gccgccgtcg gttccgtcta catcgcccga atttgtcgag gcgcagtttt
    18121 tggataccta tcgactgttt gtgccgcgtg actttaccag ccaggaaaaa gacattcgct
    18181 ggtcgcgcta ttaccaaggt cgctgggtgc gctggactgg gcagcttcgc tttgtgaccg
    18241 ccgacgcatt tttgttccac cacctgggcc cgacgagcag ctacgaagtt tcgctgcaaa
    18301 tccccgagcc agagcgctca cggctgcgac agacattgca gcttggacgg ttttataact
    18361 atgtgggacg gcttctgcgc tacgaaacca cgttccgggt gattgccctc gaacaaggct
    18421 cggttctttc gccaaacgac attggtgttc cgggcacttt ggcgtcgctg ccctggtccc
    18481 atgatccgcc cccgctccca ggccttgtgc gccccggctc caagtagcca cgtttcccgc
    18541 aaaatcgcac caaaatcgta agcttcaaaa ccgataaaat ccttctctaa cgcggagatc
    18601 cttgatactc tgcttgcaaa gccattcgta gcctggccgc acctgctggg gggatggttt
    18661 gggaagctgg ctatgtcgcg cgacctctcg ggcaagacgc taagtggata tcgtttcggt
    18721 gatgtactcg ccaaaggcgc gcaaagcacc gtctacgctg cgtaccaagg tgatggagac
    18781 cgtgccgtcg ctgtcaaagt ctttctaagc gagctaccga aagacaagac cgcagcgggc
    18841 cggattgttg ccgacgtcga gcgagccgcg cgcgtcagcc acaaaaacct ggtgccagtc
    18901 atcgaagttg gcagcctcga atggaaaggc aagcggcacc tgtttgtcgc gatggagcga
    18961 ctgcacggtg agtcgctcaa agcgcggttg caaagtcagc cagggcactc gctgccgctc
    19021 cacgttgcac ttcagatcgc aagtgcagtt ggcggcgcgc tgcaagccct gcacggcgcg
    19081 gatctggtcc atcgcggact gaatccgggc gcggtgtttt tgtcaccgcc aaccgatgcg
    19141 gaacgtcagg ccgaccttga agccgaggat cacatctatc tgctggatgc ggggcagtcg
    19201 gcgctgtttg aaggactgaa ccccagcagc aacggcaagc gtccaaagcc cagcgatgac
    19261 attcgcggcc tggctgcgct ggtttccgaa atgctgggcg gtgtgccaca gacggcagcc
    19321 caaggcagtg aagcgctttt gccgctgcgt ttccgcaacc gcaagattcc ggcgcgtctt
    19381 gatgcggtcc tgcgtctggt tctgtccagc aagctgggga cagacaagca agatccctat
    19441 ccatcggtca gcgcatttgt tgccgcgctg tttggtgatg cgaccaacat cccgacgatg
    19501 gccgcgtggt ctagcgatgg acgtgcgatg gtgcagccca cccgcaatcg cagcgggctt
    19561 atgtgggctg ccatcctttc ggtcgtcggt ggtgcaagct ttggtgtagg gtactggctc
    19621 tatcaggagc cgccgcaaac accgaaagct gacccggcgc ccgtgatttc cgtgccacca
    19681 ccggcagccg aggccctcga catggccaaa agcgccgcgg caccctctgc aaacagcgca
    19741 aatccgtcta cgggaccggc agcagcgacg gctcccgcta aaagtgaggt aaagtaagtg
    19801 aagtgccaag ctctacgcat ggttctgctt gcaggtcttc tgccaggcct tctgtccact
    19861 gctccggccc tggcacagca gccagccgca cctgccgccg ccgccaagcc gaccacaccg
    19921 gcagcgcccg ccgcagccgc caaaccgcct gtggcgatca atccacagac ggcagccttg
    19981 gctgaaaaag cgatcggtct ggcgcaaaag gatcagtacg aggaagcgct caagctgttc
    20041 cgcgatgcgt accggatcga tcgcaatccg cgctggctct acaacgtcgg cgtgctgtac
    20101 gaccggatgg caaactgcga tgaagcggcc tttttctatc gcgccgcact gtttggccag
    20161 ggtgtgctgc cgcaggacgt agacctggtc gacaaccggc ttggtgtgct tcaggaagaa
    20221 tgcaacttcc gcgagcgcca caagcaggtc gccgaccgtc acgcgcgtgc cacccgttac
    20281 atgagccccg agtttcggct ctgcgccctt gcggagtcga tcctgaccgg catcatgacg
    20341 ccgcaggaac gtcagcagct ggccacctgc aaggcgaccg aaaacacgcc cagcccgtaa
    20401 cctttccgtc gcatcgcgct gacgtcaaac ttccgccacc acacagcctt tgcgcgatac
    20461 catgggcgaa tgtcgatgaa acgccctgcc ctcgcctctc gcatcttccg caccgcgggt
    20521 tcgctggctt tgcttgctgc gtcgcttgca ccggccacat cgtcgggcta cgcgatccaa
    20581 atccacaatg aactggcgcg gcgcgctctg ctcaaaagca acctgcagga tgcggcgtcg
    20641 ccgatttctc ccgctgcgtt cgatcaaatg cggacgatca tagatagctt tgcgcgaaac
    20701 gagccgacgc tgcgtgacgc ctggcaaaag cgctatccaa ctgccgcaaa ctttgatgcc
    20761 ttcgcggaaa aacaactgct ccagcttgcg gcgtcgagca aagtctttgg catcgacgcg
    20821 gtggacgaac ggctaaaggc aggcgcgacg ctgctggatg tggtgtcatg gggcgctggg
    20881 cagcccgacg acgactggcg gaatcgcgaa cggctcgcgt atgacgacaa gcgccagccg
    20941 ctcaaagaca aatatgggca gccggtgccc gcggacccgg cgctgctcaa catgggacgg
    21001 ctcggcgcgc tgtcgagcca ggcgcatgcc cactatggac tcgcgcaggt cgagttttcc
    21061 ggggatgccg acgtgctcaa gaaagagcca aagcggtttg cccgtgccgt cggctgggaa
    21121 aaagggccga tcatcacgct cgccgctgac atggcgcaga ttcacctcga tatgtcactg
    21181 gcttgcgcgc tgagtgacgt gccgaccgcg cgtgagctgt cctggcagtt taccggcgaa
    21241 ggctttcact acctgggcga cgtcggcaac cagatccata ccgtgcaggt gggtctatac
    21301 gacttcttcg tcgacgccgc gcttgaacga ctgaaaatgg gcctgctgag cggtggcggc
    21361 tacctgggca agatgcggtc gctcggttcg attggtatcg acatcctgtc gagccaccac
    21421 acgctcagcg aagagttcac ctatcgtcgc ttcaaggctg cggtggaagg caaagcgacg
    21481 cccgaaaccg agcggctgct cagtgcacca accgaagacg accccgagtt tgccaaaaag
    21541 ctcgacgaag cacttgccaa gctgggtcca aaccccgagc gtggtgagtt tgccatggtg
    21601 ctcacccgga cgatgattga tgtgtcatcg gatcaagggg acgatgtcta ccgcgcgacc
    21661 gctggcattg gtgatccggc gctgcgcagt cggcacaaga cctttgacga agagcacgac
    21721 gatccggaaa agtttgtccg tccgcgcgat gcctcgaccg agtccgccta caaagagttc
    21781 tggagcctgc aagagcaagc gttccgtcgg gttggaacgg ccaaaaggcg ctgggtgcag
    21841 cttcagcaga agctgcttgc ctcggcgacc acccccgaac aaaaagaggc gctgcgcaaa
    21901 gaggtgctaa agcgcctgct cgacaggcag ctcaagatgc ttgatgcaag tgatgcacgt
    21961 cttgcgcagt atctggccga tccgccacag aacatcagcg cccccgaaaa agcgcctgag
    22021 ctgcttggtg ccgacgtcgt catcatcggt ctgctggtcg gcattccgct catcatcgtg
    22081 cgccgccgcc aaaaagcgca gtccaagccg caagcctagg tctagaccag cccggaaccc
    22141 tgaaggacat gtcccagtgg tggtctagac cagccccaag ccctgatagg catgccgcag
    22201 cggaggtcta gaccatcccc aaagcctgaa ggacatgccc aagtggtggt ctagaccagc
    22261 cccaaagcct gaaggacatg ccgaagtgga ggtctggacc agccccaaac cctgaaggac
    22321 atgcgcaagt ggaggtctgg accagcccca aaccctgaag gacatgccca agtggtggtc
    22381 tagaccagcc ccagacctga aggacatgcc caaggggcct tatcgcccat ctgcgtgggc
    22441 ctacatcgag tgagagcgct gggtcattga tttggctccc atctggactg atggacactg
    22501 gcgccgcttg tcgtcgctgc cctgccctat cggtgtgtcc ctacgggctg gacagtgggg
    22561 gacagttggc cgctagggcg cgggaaggtc gggctgcgcg ggaagctgcg cgccgtgcca
    22621 cacggacacg atgcgcacca cgttgtttcg ttcgtcgatc tcgtagaaga agcggtatgg
    22681 ctcgacgata aactgccgca cgggcaaggt cgcaaactcg gcaatgtagt gaccgctgcg
    22741 cgggaatctg ccaagctgcc gcagtcgcgc atgtagtcga gccgcaaagc gctgcgaggc
    22801 gaagcggttt acttctgcga tgcgatcaaa cgcgagccgg tactgagcgg ctgccttgga
    22861 cgtaaactcg actctcatgc aggcggtagg ccgtgctttt ggcggcattc gcggtcgagc
    22921 acttcccagg cgatgactcg tccggcgcgt ccgtccgcga tgccctcttc gatgctggca
    22981 aacagcgccg cccgttccgc cggatcgctc ggaagcggcg gcggcaccgg gtccatcatt
    23041 tcgccgtcac ctggctgcgt gcgtccgcgc tgcgaaccaa ccg
//
LOCUS       DAORGN010000064        22922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_884_length_22922_cov_5.321992, whole genome shotgun sequence.
ACCESSION   DAORGN010000064 DAORGN010000000
VERSION     DAORGN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22922
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_22922_cov_5.321992"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1119
                     /locus_tag="PKL17_09235"
     CDS             76..1119
                     /locus_tag="PKL17_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK44953.1"
                     /translation="MQSVPETEPHKSPVFLRGLGGIVGQDNVVSVLRSALIAKRPHHA
                     YLFDGPEGVGKATTARALAAALNCLAPPAPGDACGSCEACHKLQAASHPDFITVNMEL
                     SGLADEIEKLLRRVAYPPFEGRAQVVLIDPADQLTAPTALTAANRLLKTLEEPRPDTH
                     FVLISTCGSGLLQTIRSRCQKLRFVPLGDALIEKALTENYGLDGKMAQAVVGLSQGSL
                     GRAVRCAQDPDQLQKRQRDAEGLLLAARGGRAQDICQLAGEVGADREEAVEVLELLWL
                     RLHSELRRQGMAQATMAACRTADAMKVVRETQTAIRRYTSAPLSLERMGRHLHTAFLR
                     GTATSGGQGRSAR"
     gene            1116..2879
                     /gene="ricT"
                     /locus_tag="PKL17_09240"
     CDS             1116..2879
                     /gene="ricT"
                     /locus_tag="PKL17_09240"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNK44954.1"
                     /translation="MSDKPEAPTGRPANEGRPRPEGHRHEGPRHEGPRRDGRSEGPRR
                     DGPRLEGQRAEGARPEGPRRDRPPRSGRPPEAGGQPRRSEDRRGGNPQPGGESQPRRE
                     PRPQGQPRPPRPEGEKTDRGPQSQPGQPRSGGGRRNRPPRSGRPQGAPGSPGPQSGPA
                     SQNSQSTPGSPSRPDAARSEGTPSSPPASNRSGSPSEPGERRRRRRRGRGGRGESAAS
                     QGGTADSRASQPAIQGAAPSGEARAEDSRRIAAQPAPRPVRPQDDDDDDEPKAQSAAA
                     SPDSDDDEASEVKRLRPVSQLELPPEVPVKMANLCAVRFRPAGMIHELDCGSQTYAEG
                     ELVLCESDRGQRVGRVEVPPRPRPMMGQRRRVLRKARPDEQGGFGDTQGGESDAYKFC
                     KARLRELSLPMKLLAVELVPSQRATFYFASEDRVDFRDLVRDLSQRLRVRVEMRQVGA
                     RDGAKAIGGIGSCGRELCCSTFLPAFQPISIRMAKDQGMVLNPSKLAGQCGRLKCCLI
                     YEHSTYKELGKTLPKVGKKVITPDGVGRVLDLDILKQRVRVYLEEGGAKSYAAAEVRP
                     ANPPQGSRETPDPGSADDLET"
     gene            2899..4068
                     /locus_tag="PKL17_09245"
     CDS             2899..4068
                     /locus_tag="PKL17_09245"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK44955.1"
                     /translation="MLCPRCASSTRSAIEPTPAGAIELDYCPSCRGIWFDRGELEALL
                     LQVRSDIQPIGLPSQSDETANRRCPRHGVQMVERRLSTARLRSSMYGQGRDVPDGVTI
                     DQCPACLGIWLDGGELGQITQAMRGDSVHPLLKRTPAPTAEPDENADRPTSGLLWAFM
                     FLTGLPVEEHQPRKNFPVVVLSLVILCVMMFFVQLGASEPEQFVRMFGLVPKLAMAST
                     RWLVPWLSYMFLHGGVVHLLGNLYFLWVFGDNVEDRLGRMRFLLLYFASGFAAALLQC
                     VLQSSSSVPIVGASGAISGVMAAYAVLFPQARLVSLIWVIKVKWKTTTYLGVWLAMQL
                     IGALSGSGGVAWWAHIGGFVAGGLIAWNFRRSHQEEVTRKLALPGTPPPKILTWY"
     gene            complement(4079..4792)
                     /locus_tag="PKL17_09250"
     CDS             complement(4079..4792)
                     /locus_tag="PKL17_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44956.1"
                     /translation="MASSSADSPQPLIPRLPFYLSALAVLVLCIGVLQMVGGLLEVNT
                     AILTERSQFISSVRDRQLALFDQVSKEGPNSLAAPLRPVAQTVLKLPRAEAEKLFLLL
                     GSDLYERRSVTVPLALLQAILGYLLASSALAALRRRFGGVASWSWACMANIPAQLLNL
                     IVMMVHANRLMRHVGLAAADALAKTTGRPVAVETADLESLLRMYTSFHTAVLAAWVLL
                     LGVLTLLLQNRQGPAPQED"
     gene            complement(4799..5098)
                     /locus_tag="PKL17_09255"
     CDS             complement(4799..5098)
                     /locus_tag="PKL17_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44957.1"
                     /translation="MRRLFLSVTFLFVSAWLLFSACERDDYRSVLPLRPLDGGSSGST
                     TDMPVDLARPPQPGDLYPPQDLSMPQDMPAKPDMGGAIDGGSMSDGSLSDGGLVG"
     gene            5210..5794
                     /locus_tag="PKL17_09260"
     CDS             5210..5794
                     /locus_tag="PKL17_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNK44958.1"
                     /translation="MLHLAQPTRPGWAAYAVAHLADILLDHAHCEKKAASTALGLMFR
                     YEERPELQTPLSQLAREELAHYEEVIERLTERGIPFRRLPAGGYAAGLHEAVRKQEPH
                     RLLDNLLTCAIIEARSCERMQLLGEELERHDPQLAKLYSGLLSSEARHFRRYVELAEL
                     YFDKAQVHARLAELMQHEAEVIARIPVEPRLHCG"
     gene            5879..7420
                     /locus_tag="PKL17_09265"
     CDS             5879..7420
                     /locus_tag="PKL17_09265"
                     /inference="COORDINATES: protein motif:HMM:NF027993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44959.1"
                     /translation="MSDYLLRGQASHVAWSGYWWPMFSSHDRDDYMNLYDEGGPLDKY
                     DEYCVASGLPNPRSKEFEGWRHFADKRLENATGYTAFWWGHCNGWAAASVLENEPTTA
                     REVKGITFEVGDQKGLLTVCHNGDPVDVIRKLGDDDAHLFHAMLVQSIGKEKRGLIFD
                     TKLDPIDPKTGEPVREVWNYPAYRYEFIYTEQGPKTWDVTAQVWFANDAVEPNFVGVK
                     NWPIEERPKVYTYRIVGSQENPTSGYWIGGSRLDHPDLMWRPQPLSVQNAAEERDPGS
                     SQTTFHPTLRTLVYSICAGEEATVSQTIRRKYQSLDLQWVVTAEEQAAYPPSLAVGDF
                     WRYSVTHQDTIGNWTRPFILHVEVVGWDKDDWVFEVGAVHQGDVIPERHVIYYTKATR
                     QLSDASPYPGGYAYTLRQDINTKAFSRSDDPALWENSPLRRLLTQVPYAWDRAGMEPV
                     QRLTFKVLGDDAIPCRKLAVGKGVQVYADHTVSHPRGNIRGMFLSADLDLVSAQLIDF
                     GRKAT"
     gene            7494..8276
                     /locus_tag="PKL17_09270"
     CDS             7494..8276
                     /locus_tag="PKL17_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK44960.1"
                     /translation="MDNSDGIKELVQAQFAGSAQQFVESKLHRQGDDLQRMVDLAELS
                     GHERVLDIATGGGHTALAFARHVREVVATDLTPKMLAVAEEYVTAQGQKNVQFMRADA
                     EALPFSDAEFDVVTARLAPHHFPNPRRFVSEVARVLLPGGRFILDDNMGPEDDELFDF
                     MDRFERWRDPSHVRALRLSEWRSICEDNRLRVIHTDPLTKKVYPYKEWTERMRLPPMQ
                     ADALAAFILASPEHVKEFFDVQVVDGKLQSLCATFGILVAVT"
     gene            8276..8761
                     /locus_tag="PKL17_09275"
     CDS             8276..8761
                     /locus_tag="PKL17_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNK44961.1"
                     /translation="MPTNDQTNLTPRAAQESLTEMTEYVLPQHANALGNVFGGQIMAW
                     VDLCAAMTAQRHAGVLAVTAFVDDLMFQNPVKVGEVVHLKAQLTATFRTSMEVEVTVT
                     GEDLSTRRRWPCLHAFLTFVCIDKNGKPVPTPPLLLDSEAARESQASGAQRRALRLRK
                     P"
     gene            8816..10474
                     /locus_tag="PKL17_09280"
     CDS             8816..10474
                     /locus_tag="PKL17_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK44962.1"
                     /translation="MRATSFAVADSVQTEDFDYVVVGGGSAGCIITEALTRDPQTRVL
                     LLEAGLHADEAPETLAADQYKYAFIHPELLWDRFSVPQAKCKNARMFQGSGRGLGGSG
                     AVNAMVYTRGSAYDYDSMGLPGWRYEDVVPDFVELEKVLGVNRLPKTQFTEACIAAAE
                     ESGFRRKEDLNDGSLCGFLGYEWMNLNPQQNQRRSSYVAFLRPHENRNNLTLRTGATV
                     SRILLAPQDGTNAVAKAEGVEYLHEGIRKRARAHKEVILSAGALESPRLLLLSGIGES
                     AQLRSAGVPMMVDLPSVGQNLMDHPNVSVFFLGKQETDCRWAQLYGFHRVGNHVPEGQ
                     ADSCFVFYSARSSFREGMLRMLPTMAFPQSIYRLGWPKKILQAMIRGAFRLGPVQRFV
                     NRMYGVVVILGKPESRGQIRIASPSASDPALIDPAYFSVAGDLETMVNGVELARKMTQ
                     SHSLTAFGNRELIPGSRSKSRDRIARFISQNAMTTYHYSGTCRMGIDADSVVDLDMKV
                     RKVTGLRVADASVIPSVPVSAMNAPSMLVGYRAAQKLIAAKESA"
     gene            10471..11742
                     /locus_tag="PKL17_09285"
     CDS             10471..11742
                     /locus_tag="PKL17_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK44963.1"
                     /translation="MNVCMLVANPIVFDGRVIRHARTLVEDGHRVTVIGVIGPNDSDR
                     ALPSDTPFSVQRLRRNRQGVWPRFVWAESALRQRLAKVVGSALPSSVLAAFPFVADLT
                     VATSALELLAMAQRARYDVFHGNDLNTLPAVAWASHLQKRPYIYDAHEFYVEESPTLS
                     EYEKRARALCEGRFAKRAAAVLTVNDLLADELAQRYHIDRPVVVRNVPPRFAVPDPQT
                     QPIGKTGTLKLLYHGTHIGLAQPGTDDVLRAMARLRDRVQITLVLRGQQSPAEEKQLW
                     QRASALGLADRIRLAPPEPGPTALLQAAVADGAEVGLAFHPPLSGNFRLATSSKVYEY
                     HMAGLAVCATDVPGNHVILDESAGVFYPFGDDEKLADVLYGLATDRTRLQALRSAARR
                     RGWSEFAWENERERLLNVYRRISAARAEHRG"
     gene            complement(11739..12311)
                     /locus_tag="PKL17_09290"
     CDS             complement(11739..12311)
                     /locus_tag="PKL17_09290"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNK44964.1"
                     /translation="MTKTQTTIGAGTKIVGSVETDGDLVVDGQLEGGPLRVAGRLLVG
                     RTGAVSCEDTEVADALVLGVFSGTLKARDVVRIHQAGRLLGEVMATRVTFVSDAQLQG
                     LLGTAVNAPAPAKAATPTPSAPKAPPLRAASATTPAPVAGTAPPPLAPPAPAPIAMPH
                     AAPPPAPPVAAPRTIPALPSIGSRSMQRRD"
     gene            12452..13861
                     /locus_tag="PKL17_09295"
     CDS             12452..13861
                     /locus_tag="PKL17_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK44965.1"
                     /translation="MAATHPLPIPDAHARATDSAPSDWTFPSYFWTANLIEMCERWAY
                     YGWFILVTVYLTSTVGYSDIQAGYLTGIFAALLYLLPFLSGAIADRIGYRPALMLALL
                     LLTAGYAGMGLFPIKHVVLLPMALVMCGGALVKPIITGTVARASTPTTRARAFSLFYM
                     MVNIGSFFGKGAAEPIRKTVGVGMVPILSATVTAIGFVCVALLYRPEEARTDKTAPSA
                     SVQDAALKLLRDLKDVLRSGRLMTLVLISSGFWIISGQMYSSMPKYVFRVIGEHASPE
                     RYANVNPITVMLTVVPITWICKKLSPIASIAIALGLIPISSLVIAVLPGIVGSSTSLH
                     PVTLAMLIGISLGGLAECFLSPRYLEYISRQAPKGKEALYMGYSNLNNFLAWMIGSIL
                     SGYLLDAFVPNPSKLSAAEQAAHALALKGNGPLPPAYAHANYLWFTFFGIGALAFSAL
                     LLFAFLTRRSDSSAPDPER"
     gene            complement(13897..14385)
                     /locus_tag="PKL17_09300"
     CDS             complement(13897..14385)
                     /locus_tag="PKL17_09300"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNK44966.1"
                     /translation="MKQITNSWTLPCTPDVYWDLYLNADYSRALYLDALGFVSYQVLH
                     SDENSRKLRLQPKLNLPGPISKLVGDAFSYEQHATIDRKAGLWTWKMVQPGDKKGIVS
                     SSGTIRVTDAGNGQCIRRDEVFASGNIFGLGSLLEATVEKEVRTTWDKEIAFFRSRLS
                     SR"
     gene            complement(14491..15384)
                     /locus_tag="PKL17_09305"
     CDS             complement(14491..15384)
                     /locus_tag="PKL17_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNK44967.1"
                     /translation="MSAATASEFLSGACHCGKVKVQIPDSSAGIVACHCSDCQKLHGN
                     FFAMLVTDKTAARWEGDSHIVWYRSSAENERGFCGHCGSRIARRPLDGTKIMISAGLF
                     ERDLSRSVVKNLWIEHKPTWYDVPRVGNMTPEEFVALALASPIESPVAQYGYSLRAAS
                     HSNKAPQVIALTWITAADASERERIRAHSRETVKDFLAAPGFISIVTGFTGLRGFTVT
                     AWEEEESMKQALRKHHAQAMKELFGQRFVASVWTSVWTPTRINRIWVRCLSCGALEDV
                     SDDHRSCSECQADMPPRPAFW"
     gene            complement(15513..17828)
                     /locus_tag="PKL17_09310"
     CDS             complement(15513..17828)
                     /locus_tag="PKL17_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44968.1"
                     /translation="MQSQRKSDHGIHQRLGRLKGRYERLPETKMSSWYSIFDLFHTVL
                     DISISGMFNSRVDHRTLQAVESQGEESSPDPALATPSVTRAEGGEPADSFVARPKFFD
                     YSQCADPEFWFDFAADVGDGFNSTYYVAALLSQPKVYPLPADPQPFPLDKTDAMAQTE
                     SKAHADRLKTGLPRGRFLLLGGDEVYPSASRKNYEQRFVRVYEAAAYSETPPDKISAT
                     HLYAVPGNHDWYDGLASFMSVFAEQRAIGDFRTQQRSSYFAIKLPHGVWIFAIDIGLA
                     GELDRRQVEHFQDLAKKHLVDGDRIILCIAEPDWVKARPNIENLRDGLFYFERKLAEA
                     LTGDSACEPDSVSPDATSQETNVPPKRKDVRVILRLAGDLHHYRRHTSNEPLHPTDQE
                     AQKGSYVPSQFRPIENITAGGGGAFLHPTHEIDEDRRGAGKRTEGSIKQYPPGLYRGD
                     ATPSGEHSEDRQGIAFEFRKAFPSLAESRKITLMNLAFGHYNLRMTLTLWMIYTLLQN
                     AGLYLFAISHYAWLVLLMGCVLAAFVLGTRSYARSEASGEIGESKQGRTREVYRRASR
                     IDKAGYSGIVHGVSHVLSFFGVFAGLVVIYRNMLDAIVEKTCPFFPESTLLKWVDAPD
                     FASMSRFVLSMAAGGICAIVSTVIFGLYLYFALNHSKLKLHANGAFASLRIADFKNFL
                     RICVTADGIQVFPIGIRKVPEDWDLEIPSMQEAGKGEKDEGQQRSLFYPKPGKFASRT
                     TPSSWYERLTKSTPLSEPFLIEGPVCIKKNT"
     gene            complement(17912..19141)
                     /locus_tag="PKL17_09315"
     CDS             complement(17912..19141)
                     /locus_tag="PKL17_09315"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HNK44969.1"
                     /translation="MNRSRLGIPQLVRGIMLLGIASCGSDPTEMMTPAPSCSDGTKNG
                     TETDVDCGGGACGKCADKKVCVAATDCSGGSCVKGVCAAPSCSDTTKNGSETDVDCGG
                     GTCGKCADSKACVAATDCTSGVCSAKLCAAASCTDTVKNGSETDVDCGGSTCKPCAIN
                     LACTTATDCSTSSCKQGLCSYATSCKDLLSGNPALIDGMYMIDPDGAGPIAPLQVLCD
                     MTSDGGGWTLVGKGREGWNWVDQGEGTADELMNNPTGNTVAYLDSTVVAAIVGNPNFM
                     AWNSSLKVHRDSGFNDDYKLRPNAATTFKWSVFSDSRHGCAGPAASTFSGTVNVSASA
                     LGLAAFNNNNVDLKDYQNTGNDCTRMFTPLWNSHNCKGGWSTGLSCSPSVAGVTNCFM
                     AGSEGHCIPHVRVWVRD"
     gene            complement(19264..19794)
                     /locus_tag="PKL17_09320"
     CDS             complement(19264..19794)
                     /locus_tag="PKL17_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44970.1"
                     /translation="MTLSLVVGLVIVVACGMGYVLLSRLPSASLAPDPERVRDGLHRL
                     TKEPQSLPSLCVVYHDLHGFHGGLSLSIWGNGKISQQAVRTPVGIPKEQVSHEDLLGL
                     VALLCEVEAWEQRVPDAMPIPDESRALLRISVGDSHSVIWERYNDMAAAQRMIRVRER
                     MKAIAWRAESFTATSA"
     gene            20078..21724
                     /locus_tag="PKL17_09325"
     CDS             20078..21724
                     /locus_tag="PKL17_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HNK44971.1"
                     /translation="MAASAILVACGSSGNSGTDPSLESADASVNDPGGGDGGNGDGGN
                     GGPGGNQDGGIDGGGTDGPTPDLSPLPPDMTCVPAATPDVPEPKFMDTNCDGIDGDAS
                     AAIFVATTGKDTNPGTMASPVLTITKALALAAAQSKTSVYVSKGTYIESVTLKSGISL
                     YGGFDESMGWQRSAANVSRIQWSGTAVSATGISAETHVESLTISALQPLSGYSTYGVL
                     VANSTGPVFLRYDQITAGDAPAGLPGTAGTAGATGPNGANGAAGIMGGMGPTLGGSGG
                     VSSCGRSGGTGGIGSSKNGQNGGPGTGGGAVATGGAGGSGGAGTTSCLFMMSVAGGNG
                     SSGNAGPSGTNGNAAASFSGGTATGIPLNSGGGGADGTSGGAGGGGGAGGGDSGGVLC
                     TADAGGAGGGGGAGGCGATGGKGGGGGGSSVGVFLFQSTVTLTDSTISSGNGGAGGKG
                     GNGAVGGAGGPGGTGGGNAGEASAGGSGGSGARGGDSGAGAGGNGGASFAVYGKGATL
                     SQTNNTMTNGAGGVGGAGGTSSAGAAPSGVSGPSMAIRLE"
     gene            complement(21780..22373)
                     /locus_tag="PKL17_09330"
     CDS             complement(21780..22373)
                     /locus_tag="PKL17_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNK44972.1"
                     /translation="MTRTQRLSAAVAGIGLLVMAVLAGYANFAVIERAAQATPHQIDL
                     ATLAMGIVAFLDVVVGIGVWGLFHIDARRSAAVAAGLRVIYAALLAWAVFRLATTAGD
                     GPSRALAFQQVFAPAIGLFGIHLIVLAWPVEVTRFASPVARRGLAAAVAVCGLGYVID
                     EATRLLLVHGTRMATVTFIGEVALMFWLIILAARGRD"
     gene            complement(22370..>22922)
                     /locus_tag="PKL17_09335"
     CDS             complement(22370..>22922)
                     /locus_tag="PKL17_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNK44973.1"
                     /translation="RGHMGTKAVARMIGALFLSAFVLYGGGTALAGRPWGLVLVVLNS
                     LGVVAIGVLLHTVVHARIPQAANGYLVARSAEAIILAIGGLLIRAKALPLGSTLALDV
                     AMVGLGFGSVPLFLSLLTERLLPRWLAGWAVFSYSALALGSLLGLSGLPVAIWSSIPG
                     GLFELVFGCWLLGGGSFSQKDDA"
BASE COUNT         4704 a         6823 c         7042 g         4353 t
ORIGIN      
        1 ctgcttgccg aggtcttccc agcgtcgcga taaccgcttg catcggggct tgcggctttg
       61 aaatactgcg cggccatgca aagtgttccc gagacagagc cgcacaagtc gccggtgttt
      121 ttacgcgggc tcggcgggat cgtcggacaa gacaacgtgg tgtcggtgct gcgcagcgcg
      181 cttatcgcca agcggcccca tcacgcctat ctgtttgacg gccccgaagg tgttggcaag
      241 gcgacgacgg cacgcgcgct ggcggcagcg ctgaactgtc ttgcgcctcc tgcgccgggc
      301 gatgcctgtg gaagctgcga ggcgtgccac aagctccagg cggcaagcca tcccgatttc
      361 atcaccgtga acatggaact ttcggggctg gccgacgaaa tcgaaaagct gctgcgccgc
      421 gttgcctatc caccgtttga aggacgggcg caagtggtgc tgattgaccc ggcggatcag
      481 cttacggccc cgacggcgct gaccgcggcc aaccggctgc tcaaaacgct ggaagagcca
      541 cggcctgata cccactttgt gctgatttcg acctgcggca gcggactttt gcagacgatt
      601 cgctcgcgct gtcagaagct gcgctttgtg ccgctcggcg atgcgctgat tgaaaaagcc
      661 ctgaccgaaa actacgggct cgacggaaaa atggcgcagg cggtggtcgg tctttcgcaa
      721 ggcagcctcg gtcgtgcggt gcgctgtgcc caagatccgg accagctcca aaagcggcag
      781 cgcgatgcag aagggctgct gttggcggcg cgcggcgggc gggcccagga catctgtcag
      841 cttgcggggg aagtcggcgc cgatcgagaa gaagccgtcg aagtgctgga acttctgtgg
      901 ctgcgtctgc acagcgagct gcggcggcaa gggatggcgc aagcgacgat ggcggcctgc
      961 aggacggcgg acgcgatgaa agtcgtgcgt gagacacaga cggccattcg ccgctacacc
     1021 agtgcgccgc tgtcgctaga acggatgggg cggcatctgc atacggcatt tttgcgcggc
     1081 accgccacat cgggcggaca gggaaggagt gcgcgatgag tgacaaaccg gaagcgccga
     1141 ccggacggcc cgcaaacgaa ggacgaccgc gcccagaagg gcaccgtcat gagggaccac
     1201 ggcatgaagg cccacgccgc gatggtcgtt ccgaaggtcc gcgccgcgac ggtccgcgtc
     1261 tcgaaggcca gcgagcagaa ggtgcccgcc ccgaaggtcc acgccgcgat cgtccgccgc
     1321 gcagtggtcg tccgcccgaa gctggcggcc agcccaggcg aagcgaagac cgtcggggag
     1381 gaaatccgca gccaggtggt gaatcccagc cgcgccgtga accacgacca cagggtcagc
     1441 ccaggccgcc ccgtcccgaa ggcgaaaaaa ccgatcgcgg accgcagagc cagccggggc
     1501 agccaaggag tggtggcggg cgtcgcaacc gtccgccgcg cagtggtcgt ccgcaaggcg
     1561 caccgggatc gccaggtcca cagagcggcc ccgcgtcgca aaactcgcag agcacaccgg
     1621 gatcacccag ccgaccggac gccgcacgca gcgaaggcac accatcgtcc ccaccggcat
     1681 cgaaccgaag cggcagtcca tcggaacccg gtgaacgtcg ccgccgtcgt cgtcgaggtc
     1741 ggggtggtcg gggcgaaagt gcagcctcgc aaggcggtac cgcggacagt cgcgcctcgc
     1801 agccggcgat tcaaggagcc gcgccaagtg gtgaagcccg cgccgaagac agccgacgaa
     1861 ttgccgcgca gcccgcgccc cgcccagtgc ggccacagga tgacgacgat gacgacgagc
     1921 ccaaggccca aagcgccgcc gcatcgccag acagtgacga cgacgaagcg agcgaggtca
     1981 agcggctgcg gcccgtttcg cagcttgagc tgccgccgga agtgccggtc aagatggcca
     2041 acctctgcgc ggtgcgcttc cgtcccgccg ggatgatcca tgaactggac tgcggcagcc
     2101 agacctacgc cgaaggtgag ctggtgctgt gcgaatcgga tcgcggtcag cgggtcggtc
     2161 gggtggaagt gccgccgcgg cctcgtccga tgatgggaca aagacgccgg gtcttgcgca
     2221 aagcccgccc cgatgagcaa ggcggttttg gcgacacgca aggcggggaa tcggacgcct
     2281 acaagttctg caaagcccgc ctgcgagagc tgtcgctgcc gatgaagctg cttgcggtcg
     2341 agcttgtccc aagtcagcgc gcgacgtttt attttgcgtc ggaagatcgg gtggatttcc
     2401 gcgacttggt gcgcgatctg tcgcagcgac tgcgggtccg ggtcgaaatg cggcaagttg
     2461 gtgcccgcga cggagccaaa gccatcggtg gcatcggatc gtgcgggcga gagctgtgct
     2521 gttcgacgtt tttgccagcg tttcaaccga tttcgatccg gatggccaaa gaccaaggca
     2581 tggtgctcaa tccgtcgaag ctggccggtc agtgcggacg gctcaagtgc tgtcttatct
     2641 acgaacactc gacgtacaaa gagctgggca agacgcttcc gaaagtcggc aaaaaagtca
     2701 ttacacccga cggagtgggc cgcgtgctcg acctggacat tttgaagcag cgggtgcgcg
     2761 tctatctcga agagggtggc gcaaaaagct atgccgccgc cgaagtccgt cccgccaatc
     2821 ccccgcaagg cagccgcgaa acccccgatc cgggctcggc agacgaccta gaaacctaga
     2881 acatgttacc gtgtgggcat gttgtgtcct cgctgtgcca gtagcacacg ctcggcgatt
     2941 gagccgacgc ccgccggtgc cattgagctg gattactgcc catcctgtcg tggcatttgg
     3001 tttgaccgtg gcgagctgga agcgctgctt ctgcaagttc gttccgatat ccagccgatt
     3061 ggtctgccgt cgcaaagcga tgaaaccgca aaccgccgct gtccgcgcca cggggtgcag
     3121 atggtggaac gcaggctgtc gacggcgcga ctgcggtcga gcatgtacgg tcaaggtcgc
     3181 gatgtcccgg acggcgtcac gattgatcag tgcccggcct gtctgggaat ttggctcgac
     3241 ggtggcgagc tgggacagat cacgcaggcg atgcggggcg acagcgttca cccgcttttg
     3301 aaacgcacgc ccgcgccgac tgccgaaccg gacgaaaatg cagaccgccc gacgtcggga
     3361 cttttgtggg cgtttatgtt tctgaccggc ctgccggtgg aagaacatca gccgcgcaag
     3421 aacttcccgg tggtcgtgct gtcgctcgtc atcctctgcg tgatgatgtt ttttgtgcag
     3481 ctaggtgcca gcgaacccga gcagtttgtg cggatgttcg gccttgtacc gaagctggcg
     3541 atggcgtcga cccgatggct tgtgccgtgg ctgtcgtaca tgtttttgca tggcggcgtc
     3601 gtccacctgc tcggcaatct gtactttttg tgggtgttcg gcgacaacgt cgaagaccga
     3661 ctgggacgga tgcgattttt gctgctgtat tttgcgtcgg gatttgcggc ggcgctgctc
     3721 cagtgcgtgc tgcaatcatc gtcgagcgtc ccgattgtcg gtgcatcggg agcgatttcg
     3781 ggcgtgatgg cggcttacgc cgtgctgttc ccccaggcgc ggcttgtgtc gctgatttgg
     3841 gtcatcaaag tgaagtggaa gacgacgacg tatctgggtg tctggctggc gatgcagctc
     3901 atcggcgcgc tgagtggcag cggcggcgtg gcgtggtggg cgcacatcgg tggctttgtt
     3961 gcgggcggac tcatcgcgtg gaacttccgc cgatcccacc aagaagaagt cacccgaaag
     4021 ctggcactcc caggcacgcc cccacccaaa atcctgacgt ggtactgacg cggggttctc
     4081 agtcttcttg cggcgccggg ccctgacgat tttgcagaag cagggtgagc acgccgagca
     4141 gcagcaccca cgcagccagc accgccgtat gaaagctcgt gtacatccgg agcagcgact
     4201 ctaggtctgc ggtctcgacg gccactggac gaccggtcgt cttggccaag gcatccgccg
     4261 ccgccaaacc gacgtgacgc atcagccgat tggcatgcac catcatgaca atcaggttca
     4321 gaagctgcgc cggaatgttg gccatgcagg cccagctcca cgacgcaacg ccgccaaagc
     4381 gacgacgcaa tgccgcaagc gcgctgctcg ccaaaagata gccgaggatc gcctgcaaaa
     4441 gcgccagcgg caccgtcacg ctgcgccgtt cgtacaggtc ggaaccaagc agcagaaaca
     4501 gcttttcggc ttccgcgcgc ggcagcttca gcaccgtctg ggcaaccggt cgaagcggcg
     4561 ccgcgagcga gtttggtccc tctttgctga cctgatcgaa cagcgcgagc tgccggtcgc
     4621 ggaccgatga gatgaactgg ctgcgctcgg tcaggatggc ggtgttgact tcgagaagtc
     4681 cgccgaccat ttgcaaaacg ccgatgcaca gcaccagcac agcgagcgcc gacagataaa
     4741 aaggaagccg cgggatgaga ggctgcggac tgtcagccga agaagaggcc atgccgggct
     4801 aacctaccag gccgccgtcg gaaagggagc cgtcgctcat gctgccgcca tcgatcgcgc
     4861 cgcccatgtc cggtttggcg ggcatgtctt gcggcatcga caggtcttgc ggcggataca
     4921 gatcgcccgg ctggggaggc cgtgccaagt cgaccggcat atcggtcgtc gatccggaag
     4981 aaccaccgtc gagcggacgc agcggcagca cactgcgata gtcgtcgcgc tcgcatgccg
     5041 aaaacagaag ccacgccgac acaaacagga aggtcacaga aagaaacagc cgccgcattc
     5101 atgctctccg tcgtaagtcg ccgcaggcta ccatcgcttt tgcgccactc gcaacattgc
     5161 ctttgcctgg gctggcacgc gccgccgatt ctgcggtagc ctgccgcgca tgcttcacct
     5221 agcacaaccg acgaggccgg gctgggctgc ctatgcggtg gcgcatcttg cggacatttt
     5281 gctcgaccac gcgcactgcg aaaagaaagc ggcgtcgacg gcgctcggtc tgatgtttcg
     5341 gtatgaggaa cggcccgaac tccagacgcc gctgtcgcag ctcgcgcggg aggaactggc
     5401 ccattacgaa gaagtgatcg agcggctgac ggagcgaggg attccgtttc ggcggctgcc
     5461 cgcaggtggg tacgcggcgg gactgcatga agcggtgcga aagcaggagc cgcatcggct
     5521 gctcgacaac cttttgacct gcgcgatcat tgaagcgcga agctgtgagc gaatgcagct
     5581 tctgggggaa gagctggagc ggcacgaccc gcagctagcg aagctgtata gtgggctgct
     5641 ttcgtcggaa gcgcggcact tccggcgcta cgtcgagctg gccgagctgt actttgacaa
     5701 agcgcaggtg cacgcccggc tggccgagct tatgcagcat gaagccgagg tgattgcgcg
     5761 catccccgtc gagccccgcc tgcactgcgg ctaagttgta ccgcggcgct acccactgtc
     5821 ggactccgcg gtaaagtagt ggggacgttt ggcaattctt cctgcgaagg agcaagcgat
     5881 gagcgactat cttttgcgcg gacaagcatc ccatgtggcg tggtcgggat actggtggcc
     5941 gatgttttcg agccacgatc gcgacgatta catgaatctg tacgacgagg gcgggccgct
     6001 cgataagtac gacgagtatt gtgtggcgag cgggctaccc aatccgcgca gcaaagagtt
     6061 tgaaggctgg cgtcactttg ccgacaagcg actggaaaac gcgaccggat acaccgcgtt
     6121 ttggtgggga cactgcaatg gctgggccgc agcgtcggtg ctggaaaacg aaccgacgac
     6181 ggcgcgggaa gtcaaaggca tcaccttcga ggtgggcgac caaaagggtc ttttgacggt
     6241 gtgtcacaac ggcgatccgg tcgatgtgat tcgcaagctc ggcgacgacg atgcgcacct
     6301 gttccacgcg atgctggttc agtcgattgg caaggaaaag cgcggactca ttttcgacac
     6361 caagctcgat ccgattgatc ccaagaccgg cgagccggtg cgcgaagtct ggaactaccc
     6421 cgcgtatcgt tatgagttta tctacaccga acagggaccg aagacctggg atgtgacggc
     6481 gcaggtctgg tttgcaaacg atgccgtcga gcccaacttt gtcggcgtca aaaactggcc
     6541 gatcgaggaa cgaccgaagg tctacaccta tcgcatcgtc ggcagccaag aaaatccgac
     6601 cagcggttac tggatcggcg gttcacggct cgatcatccg gacttgatgt ggcgaccgca
     6661 gccgctgtcg gtgcaaaacg ccgccgagga gcgcgatccg gggtcgagcc agacgacgtt
     6721 ccatccgacg ctgcgcacgc tggtgtactc gatctgcgcg ggcgaagaag cgaccgtttc
     6781 gcagacgatt cgccgcaagt accaaagcct ggatttgcag tgggtggtga cagcggaaga
     6841 acaggctgcc tatccgccgt cgctcgccgt cggtgatttc tggcgataca gcgtgacgca
     6901 ccaggatacg atcgggaact ggacgcggcc ctttatcctc cacgttgagg tcgtcggctg
     6961 ggacaaagat gactgggtgt tcgaggtcgg cgccgttcac caaggcgatg tgattccgga
     7021 acgccacgtc atttactaca ccaaagcgac gcggcagctt tccgatgcat cgccctatcc
     7081 gggtggctat gcgtacacgc tgcggcaaga catcaacacc aaagcgtttt cgcgcagcga
     7141 cgatcccgcg ctgtgggaaa acagtccgct gcggcgactt ctgacccagg ttccctatgc
     7201 ctgggatcgc gccggaatgg aaccagtgca gcgtctgacg ttcaaagtcc tgggtgacga
     7261 cgcgattcct tgtcgcaagc tcgcggtggg aaaaggcgtg caggtctatg ctgatcacac
     7321 cgtcagccat ccgcgcggca acatccgcgg catgttcctg tccgccgatc tcgatcttgt
     7381 gtccgcccag ctcatcgact tcggacgcaa agcaacgtaa gacgcggaaa cagtctcgca
     7441 gggaagcgag gccccacgca aaaacgtcta cgcgccaagg aggcattcct aagatggaca
     7501 acagcgatgg catcaaagaa ctggtgcagg cgcagtttgc cgggtcggcg cagcagtttg
     7561 ttgaaagcaa gctccaccga caaggcgatg atctgcagcg catggtcgat ctggccgagc
     7621 tgtccggaca cgagcgcgtg ctcgacatcg cgaccggggg cggacatacc gcacttgcgt
     7681 ttgcgcgtca tgttcgcgaa gtggtcgcga ccgatttgac ccccaaaatg ctcgccgtcg
     7741 ccgaggaata tgtcaccgcg cagggacaga aaaacgtcca gttcatgcgt gccgatgcag
     7801 aagcgctgcc gttttccgat gcggaatttg atgtggtgac agcgcgactg gctccgcatc
     7861 actttcccaa tccgcggcgt tttgtgtccg aagtcgcgcg tgtccttctg cccggcggac
     7921 gcttcatact cgacgacaac atgggtccgg aagacgatga actgtttgac tttatggatc
     7981 gcttcgaaag atggcgtgat ccaagtcatg tgcgcgcgct gcggctttcg gaatggcgat
     8041 cgatctgtga agacaaccgc ctgcgggtga ttcacaccga tccgctgacc aagaaagtct
     8101 atccgtacaa ggaatggacc gagcggatgc gactcccacc catgcaagcg gacgcgctcg
     8161 cagcgtttat tttggcaagt ccagagcacg taaaagagtt cttcgatgtg caagtggtcg
     8221 acggaaaact acagtcgctg tgtgcgacgt tcggaatcct tgtcgcggtg acgtgatgcc
     8281 cacaaatgac caaaccaact tgacgccgcg cgcagcccag gaatcgctga ccgaaatgac
     8341 cgagtatgtt ctgccgcagc acgccaacgc gcttggcaat gtgttcggtg gacagatcat
     8401 ggcgtgggtc gatctgtgcg cggcgatgac ggcgcaaaga catgccggag tgctcgcagt
     8461 cacggcattt gtcgacgatc tgatgtttca aaatccggtc aaagtcggtg aagtcgtgca
     8521 cctcaaagcg cagcttaccg cgacatttcg cacgtcgatg gaagtcgaag tcaccgtcac
     8581 aggggaagac ctgtcgacgc gacggcgctg gccttgtctg catgcatttt tgacgtttgt
     8641 ctgcatcgac aagaacggaa agcccgtgcc aacgccgccg cttctgctcg attccgaagc
     8701 agccagggaa tcgcaagcca gcggtgccca gcgacgagcc ctgcgcctgc gcaagcccta
     8761 aaaaatatga tgacagtcga aagcgctggg ccgttttgca gtaggatgcg cggccatgcg
     8821 cgcgacttct tttgctgtgg cagattccgt tcaaacagaa gacttcgatt acgtggtggt
     8881 gggcggcgga tcggctggct gcatcatcac cgaagcactg acccgcgatc cacagacacg
     8941 ggtgctcttg ctcgaggccg gactccacgc tgatgaagca ccggaaacgc tggctgccga
     9001 tcagtacaag tacgcattca ttcatccaga gcttctgtgg gatcgctttt cggttccgca
     9061 agcaaagtgc aaaaacgcgc ggatgttcca gggttcagga cgcggacttg gtggcagtgg
     9121 cgccgtcaac gcgatggtct acacccgtgg cagcgcgtat gactacgaca gcatggggct
     9181 gcccggctgg cgttatgaag acgtcgttcc ggattttgtc gaactggaaa aagtgctcgg
     9241 ggtgaatcgg ctaccgaaaa cacagtttac cgaagcgtgt atcgccgctg cagaggagtc
     9301 cggtttccgt cgcaaagaag acctgaacga tgggtctctg tgcggttttc ttggctatga
     9361 atggatgaac ctgaatccgc agcaaaacca gcggcgcagc tcctatgttg cgtttctgcg
     9421 tccgcatgaa aaccggaaca acctgacgct gcgcaccggg gcgacggtgt cgcggattct
     9481 gctcgcgccg caagatggga caaacgccgt cgccaaagcc gaaggagtcg agtatctgca
     9541 cgaaggaatc cgcaagcggg cgcgcgcgca caaagaagtg attctgtctg cgggggcgct
     9601 ggaatcgccc aggcttttgc tgctgtccgg aatcggtgaa tcggcacagc tccgctccgc
     9661 aggcgttccc atgatggtcg atttgccgtc ggtgggacag aacctgatgg atcatcccaa
     9721 tgtcagcgtg ttctttttgg gaaaacagga aacggactgc cgctgggcgc agctgtatgg
     9781 gttccatcgg gttgggaatc acgttccgga aggccaagcg gattcctgtt ttgtttttta
     9841 cagcgcgcga tcttcgtttc gggaaggcat gctgcggatg ctgccgacga tggcttttcc
     9901 acagtcgatt taccgtctgg gttggccgaa aaaaatcctg caagcgatga tccgcggtgc
     9961 attccgtttg ggtccggtgc agcgctttgt caatcgaatg tacggcgtgg tggtgatctt
    10021 gggcaaacct gaaagccgcg gtcagattcg cattgcatcg ccttccgcaa gtgatccggc
    10081 gctgattgat ccagcctatt tttccgtcgc tggcgatctg gaaacgatgg taaacggagt
    10141 cgagctggcg cgaaaaatga cccagtccca cagtctgact gcgtttggga atcgcgagct
    10201 gattccgggg tcacgcagca agtcgcgcga tcggattgcg cgctttatca gtcaaaatgc
    10261 catgacgacg tatcactact cgggaacgtg ccggatggga atcgatgcag actcggtggt
    10321 ggatttggac atgaaagtgc gcaaagtcac aggtctgcgc gtcgccgatg catcggtgat
    10381 cccgtcggtt ccggtgtctg cgatgaatgc gcctagcatg ctggttgggt atcgcgccgc
    10441 ccaaaaactg atcgccgcca aggagtccgc atgaatgtct gcatgctggt cgccaacccg
    10501 atcgttttcg atgggcgggt gatccgtcat gcgcgcacgc tcgtcgagga tggtcatcgc
    10561 gtcacggtga ttggcgtgat cggtcccaac gattccgacc gcgcgctgcc aagcgacacg
    10621 ccgttttctg tgcagcggct gcgacggaat cggcaaggag tctggccgcg atttgtctgg
    10681 gccgagagtg cgctgcggca gcggctggcg aaagtcgtgg ggtctgccct gccctcttct
    10741 gtcttggccg cgtttccttt tgtggcggat ctgacggtgg cgacatcggc gctggagctt
    10801 ttggcgatgg cgcaacgcgc gcgctacgac gtgttccacg gcaatgatct gaacacgctg
    10861 ccggcggtgg cgtgggcttc acacctgcaa aaacgaccgt acatctacga tgcgcacgaa
    10921 ttttatgtcg aagaatcccc gacgctgagc gagtacgaaa aacgcgcgcg ggcgctgtgt
    10981 gaaggccggt ttgccaagcg ggccgccgcg gtcttgaccg tcaacgatct cctggcggat
    11041 gagcttgcgc agcggtatca catcgatcgt cccgtcgtcg tgcgcaatgt gccgccgcgc
    11101 tttgcggtgc ccgatccgca gacgcaacct atcggaaaga cgggaacgct caagcttctg
    11161 tatcacggca cgcacatcgg tcttgcgcag ccaggcaccg acgacgtgct gcgggcgatg
    11221 gcacgactgc gcgatcgggt gcagatcacg ctggtgctgc gcggtcagca gtcgccggcg
    11281 gaagaaaaac agctttggca aagggcaagc gcgctcgggc tggcggatcg cattcgattg
    11341 gcgccgcccg agccaggacc gaccgcactt ctgcaagccg cggtcgcaga cggagccgaa
    11401 gtcggtctgg cgtttcatcc accgctttcg ggcaattttc ggctcgccac gtcgagcaaa
    11461 gtctacgaat accacatggc ggggcttgcg gtctgtgcga ccgatgtgcc cggcaatcat
    11521 gtgattctcg acgagagcgc cggtgttttt tatccgtttg gcgatgatga aaaactggcc
    11581 gatgtgctgt acggactcgc aaccgatcgc acccgactgc aagcgctgcg cagcgccgca
    11641 cgacgacgcg ggtggagcga gttcgcctgg gaaaacgaac gcgagcggct gctcaacgtc
    11701 taccgacgga tttccgccgc acgggcggaa catcgcggct agtcgcgacg ctgcatcgag
    11761 cgagatccaa tcgacggcag ggccggaatc gtgcgcggag ccgcaaccgg aggagccggc
    11821 ggtggggccg catgaggcat cgcgatcggc gcgggagccg gcggggcgag cggcggcggt
    11881 gccgtcccag cgacgggcgc cggcgtcgtg gcagatgcag cccgcaaagg tggagctttg
    11941 ggtgcgctgg gggtcggcgt cgcggccttg gccggagccg gggcgttcac cgcagtcccg
    12001 agcagaccct ggagctgcgc gtccgaaaca aaggtaactc tcgtcgccat cacttctccg
    12061 agcagccgac ccgcctggtg aatccgcacc acgtcacgcg ctttgagcgt tcccgaaaaa
    12121 accccgagga cgagtgcgtc cgcaacctcg gtgtcctcgc acgagactgc gcccgtgcgt
    12181 ccgaccaaca ggcgtccagc gacgcgcagt gggcctcctt ctagctgtcc atcaaccacg
    12241 aggtctccgt cggtttcgac gctgccgacg attttggttc cggccccgat ggtggtttgt
    12301 gtcttcgtca tggtgccgca gaataaagcc gcgcgctgtc cgacgcaatg aaccttcgca
    12361 cgcgcgacgc ttcgcaagcg gatggatctt gttcgataat gaaaagatgc tggcatctcg
    12421 atcgcctagc tccgtccccc aggccgtggc cgtggccgca acccacccgc tcccgatccc
    12481 cgacgcccac gcccgcgcaa ccgacagcgc cccgtccgac tggaccttcc catcgtattt
    12541 ttggaccgcc aacctcatcg agatgtgcga gcgctgggcc tactacggct ggttcatcct
    12601 cgtcaccgtc tacctgacat cgacggttgg ctacagcgac atccaggccg gctacctgac
    12661 cggcatcttc gcggcccttt tgtacctgtt gccgtttttg tcgggagcca tcgccgatcg
    12721 gatcggctac cgcccggccc tgatgctggc gctgctgctg cttaccgccg gatacgccgg
    12781 gatgggactg tttccgatca agcacgtcgt gcttctgccg atggcacttg tgatgtgcgg
    12841 cggcgcgctg gtcaaaccga tcatcaccgg caccgtcgcc cgcgcatcca cccccaccac
    12901 ccgagcccgc gcttttagcc tgttttacat gatggtcaac atcgggagtt tttttggcaa
    12961 gggtgcggcg gaaccgattc gcaagacggt tggggttggc atggttccta ttctgtctgc
    13021 aaccgtcacc gccatcggct ttgtgtgcgt ggcactcctc taccggccag aggaagcacg
    13081 gacagacaag actgcgccga gcgcatcggt gcaagacgcg gcactgaaac tgctgcgcga
    13141 tttgaaagat gttctgcgct cgggccgctt gatgacgctc gtgctcatct catcgggatt
    13201 ttggatcatc tccggtcaga tgtactcctc gatgcccaag tacgtcttcc gcgtcatcgg
    13261 tgagcacgca agtccagaac gttacgccaa cgtcaatccg atcaccgtga tgcttaccgt
    13321 cgtaccgatc acttggatct gcaaaaaact ctcgccgatc gcttccattg cgattgcgct
    13381 cggtctgatt ccgatctctt cgctggtcat cgccgtactg ccagggatcg tcggatcgtc
    13441 cacatcgcta catccggtca cgctggcgat gttgattggg atttcgctcg gcggactcgc
    13501 agagtgtttc ctgagcccac gctaccttga atacatctcg cgccaggctc ccaaaggaaa
    13561 agaagccctg tacatgggct actcgaacct gaacaacttc ctcgcatgga tgattggatc
    13621 gattctgtct ggctatctgc tcgatgcctt cgttcccaat cccagcaaac tctctgctgc
    13681 ggaacaggca gcgcatgcgc ttgcgctgaa aggaaacgga ccgcttcccc cagcctacgc
    13741 gcacgcaaac tatctgtggt ttacgttctt cggaatcgga gcgctcgcct tttcggcgct
    13801 cctcttgttt gcatttctca cacggcgcag cgattcatcg gcccctgatc cggagcgata
    13861 gacgactccc ttttttcgct ccgcaggaag acatccttag cgggacgaca ggcggctgcg
    13921 gaaaaacgcg atctctttgt cccacgtagt acggacttcc ttttccaccg ttgcttcaag
    13981 caggctgcca agcccaaaga tgttgccaga ggcaaagact tcgtcgcgac ggatgcactg
    14041 cccgtttcca gcgtcagtta cgcgaatggt tccgctgctg gaaacgatgc cctttttgtc
    14101 ccccggctgc accattttcc aggtccacag acccgccttg cgatcaatcg tcgcgtgctg
    14161 ttcatacgaa aatgcgtcac cgaccagctt ggatatcgga ccgggcagat tcagcttggg
    14221 ctgaagccgc agcttgcgcg agttttcgtc gctgtgcagc acttgatagg aaacaaatcc
    14281 cagcgcatcc agatacagcg cacgggaata atccgcattc aagtacaagt cccaatagac
    14341 atccggcgtg cagggaaggg tccagctatt tgtgatctgt ttcatggcgt tttgcagaag
    14401 aactgagcac aaccgaacca ccccgtcaac caccaggcag agcgcaatcg gaatctgatc
    14461 ggaatcagaa cagaagcgag gagagagggt ctaccaaaac gctggacgcg gcggcatgtc
    14521 tgcctggcat tcgctacagc ttctgtgatc atcggataca tcttccagcg cgccgcaaga
    14581 cagacagcga acccagatcc gattgatgcg cgtgggcgtc cacacactgg tccacaccga
    14641 cgcgacaaag cgctgaccaa acagctcttt catggcttgt gcatggtgtt tgcggagcgc
    14701 ctgcttcatc gactcctctt cttcccatgc agtcacggtg aagccgcgca gcccagtaaa
    14761 accggtcacg atcgaaatga atccaggcgc ggccagaaag tccttcacgg tttcgcgcga
    14821 gtgcgcccgg atgcgttccc gttcactcgc atcggcagcc gtaatccagg tcagtgcaat
    14881 cacctgaggg gccttgttgc tgtgtgatgc cgcgcgcagc gaatagccgt actgcgcgac
    14941 cggactctcg atcggagaag ccagtgccag ggcaacaaac tcttccggcg tcatgttgcc
    15001 gacccgcggc acgtcgtacc aagtgggctt gtgttcgatc caaagattct ttacgaccga
    15061 tcgcgacaga tccctctcaa acagtcctgc cgaaatcatg atctttgtgc catcgagcgg
    15121 acgcctggcg atgcgactgc cgcagtggcc gcagaaaccg cgctcatttt cagcagagga
    15181 acgataccaa acgatgtggc tgtcgccttc ccacctggcc gccgtcttgt ccgtgaccag
    15241 catcgcaaag aagttgccgt gcagcttctg acagtcgctg cagtgacacg ccacaatccc
    15301 tgcgctgctg tcgggaatct gcacctttac tttgccacag tgacacgctc cggaaaggaa
    15361 ctcgctcgca gtcgcagcac tcatcgtcat acctccgaag agacaaatat ggcgctgcga
    15421 caacttgcag acaatgcaag tttctgaaac gtatcatttc aaattgtgca acatcaaacc
    15481 ggcgtgcaaa tccaccgcgc gaaacccccg atctaggtgt ttttcttgat gcagaccggg
    15541 ccttcgatca aaaagggctc gctgagtgga gtcgactttg tgagccgctc gtaccacgat
    15601 gaaggagtcg tccgcgacgc gaacttcccc ggtttgggat agaacagaga gcgctgctgg
    15661 ccttcgtctt tttcaccctt ccctgcctcc tgcatagaag gaatctccaa atcccagtct
    15721 tcgggtactt tccgaatccc gattgggaag acttggatgc cgtcggcggt gacacaaatc
    15781 cgcaggaagt ttttgaagtc ggcgatgcgc agcgatgcaa acgccccatt tgcgtggagt
    15841 ttcagcttgc tgtggtttag cgcaaagtac agatacagac cgaagatcac cgtgctgacg
    15901 atcgcacaga ttcccccggc agccatcgat aggacgaagc gcgacatcga agcaaagtcc
    15961 ggagcatcga cccattttag cagcgtcgat tctggaaaga atggacaggt cttttcgact
    16021 atcgcgtcca gcatgtttct gtatatgacg accagaccgg cgaacacgcc gaaaaaggac
    16081 aggacatgtg acacgccgtg cacgatgccg ctgtagcctg ctttgtcgat ccggctcgct
    16141 ctgcgataga cttcgcgcgt ccgaccctgt ttgctttcgc caatctcgcc gctcgcttcg
    16201 ctccgtgcgt agcttcgggt accgagtaca aacgccgcca gcacacatcc catcagcaag
    16261 acaagccacg cataatggct gatggcaaaa agatacaaac cagcattttg cagcagcgtg
    16321 tagatcatcc acagcgtaag cgtcatccgc aggttgtagt gaccaaacgc caggttcatc
    16381 agcgtgatct tgcgcgactc tgcaaggctt gggaacgcct tgcggaactc gaacgcaata
    16441 ccttggcggt cttcgctgtg ttccccactt ggggtcgcgt cgcctcgata tagtccgggc
    16501 ggatactgct tgattgaccc ttccgttcgc ttgccggcac cgcgtctgtc ttcgtcaatt
    16561 tcatgggtcg gatgcaaaaa tgcgccgcca ccccctgcgg tgatgttttc gatcggacgg
    16621 aattgggaag gtacgtagga ccccttttgc gcttcctgat cggtgggatg cagcggttcg
    16681 ttcgaggtat ggcgtcgata gtgatgaagg tcgcccgcga gccgcaaaat gacgcgcacg
    16741 tctttccgtt tgggagggac gttcgtctcc tgcgatgttg catcggggga aacggaatcc
    16801 ggttcacaag cagaatcccc tgtcagcgct tccgccagct tgcgttcaaa ataaaacagg
    16861 ccatcgcgca ggttttcgat gttgggacgc gccttcaccc agtccggctc ggcgatgcac
    16921 aaaatgatac ggtccccgtc gacaagatgt ttctttgcca agtcctgaaa gtgttcgacc
    16981 tggcggcgat ccagctcacc tgcaagacca atatcaatcg caaaaatcca cacaccgtgc
    17041 ggcagcttga tcgcaaaata actgctgcgc tgctgggtgc gaaaatcacc gatggcgcgc
    17101 tgctcggcaa acaccgacat aaagctcgca aggccgtcgt accagtcatg attccctggc
    17161 acggcataca ggtgcgtggc actgattttg tcaggaggag tttcgctgta cgcagcggct
    17221 tcatagacac gaacaaagcg ctgttcatag tttttgcgcg acgcgctggg atagacttcg
    17281 tcaccgccca gcagcaaaaa tcgcccgcgc ggtagacctg tcttcagtct gtcggcatgg
    17341 gctttggatt ctgtctgtgc catcgcatcc gtcttgtcca aaggaaacgg ctgcggatca
    17401 gcaggaagcg gatagacctt tggttgcgac agcagcgcag cgacgtaata cgttgaattg
    17461 aaaccatcgc caacatcggc ggcaaagtcg aaccaaaatt ccggatcggc gcattggctg
    17521 tagtcaaaaa acttcggacg cgcaacgaag gagtccgccg gttctccccc ctccgctcgg
    17581 gtcacagagg gtgtcgccag cgcagggtct ggcgagctct cttcgccctg gctttcgacc
    17641 gcttgcagcg tgcggtgatc gacccgtgaa ttgaacatgc cagagatcga aatgtcgagg
    17701 accgtatgga acagatcgaa gatgctgtac caactggaca tcttggtttc cggcaagcgc
    17761 tcgtaacgac ctttgagacg ccccaaccgc tgatgaatgc catgatcaga cttccgctga
    17821 ctttgcatgc gcgggattct atttcaggaa acagcggaag caaacgattc gatcggagag
    17881 agatatggaa aaggacgaaa gaccggagtg attagtcgcg gacccaaacg cgaacgtggg
    17941 ggatgcagtg tccttcgctt cccgccatga agcagttggt cacgccagca accgacggtg
    18001 agcagctgag gccggtcgac cagccgccct tgcagttgtg gctgttccac agcggagtga
    18061 acatgcgcgt gcagtcattt ccggtgttct ggtagtcctt cagatcgacg ttgttgttgt
    18121 tgaacgcggc cagacccagt gcgctggcgc tgacgttcac cgtcccgctg aaggtgctgg
    18181 cagcagggcc cgcgcagccg tgtcggctat cggaaaacac actccacttg aaggtcgtcg
    18241 cagcgttcgg acgcagcttg taatcatcat tgaagccact gtcgcgatgc actttcagac
    18301 tgctgttcca tgccatgaag ttgggattcc caacgatcgc cgcaaccacc gtggaatcca
    18361 gatacgcgac ggtatttccg gtggggttgt tcatcagctc atcggcggtt ccttcgcctt
    18421 gatcgaccca gttccagccc tcacgaccct tgccaaccag cgtccagccg cccccatcgc
    18481 tcgtcatgtc acagagcacc tggagcggcg caatcgggcc cgcaccatcc ggatcgatca
    18541 tgtacatgcc gtcaatgagc gccgggtttc cggaaagcag gtctttgcag cttgtcgcat
    18601 acgaacacag tccctgcttg cagctgctgg tcgagcagtc cgtggcagtt gtgcaagcaa
    18661 gatttattgc acacggcttg caagtgctgc cgccgcagtc aacatccgtt tcgcttccgt
    18721 tcttgacggt gtcggtgcag ctggcggcgg cacaaagctt ggctgaacag actccgcttg
    18781 tgcagtcggt ggctgcgaca cacgctttgg aatcagcgca ctttccgcag gttccgccgc
    18841 cacagtcgac atccgtttcg cttccgtttt tggtggtatc tgaacaggaa ggagccgcgc
    18901 acacgccttt tacgcacgat ccgccgctac agtccgtggc cgctacgcac acctttttgt
    18961 cggcgcattt tccgcacgcg ccaccgccgc agtccacgtc ggtctccgtg ccgtttttgg
    19021 tcccgtccga acaggacggc gcaggcgtca tcatttcggt tggatcgctg ccacaagatg
    19081 caatgcccag cagcatgatt ccccggacaa gctgtggaat gccaagtcga cttctgttca
    19141 tttccgtatc cccctggtgg aagttaaagc gcgaaaaaca cgccagacgc cgaagatcat
    19201 aaccgccccc acgcgccggg tcagagcaaa tctggacaag aagcaaaaat agctggttgc
    19261 tcgtcacgca gacgttgcag tgaaggactc cgcacgccac gcaatcgctt tcatccgctc
    19321 gcgcacgcgg atcatccgct gggccgccgc catgtcgtta tagcgttccc agatgaccga
    19381 atgagagtcc cctacagaga tccgcagcag cgccctgctt tcatcgggaa tcggcattgc
    19441 atcgggcact cgctgttccc aggcttcgac ttcacacaga agcgccacca gcccgagcaa
    19501 gtcctcatga ctgacctgtt ctttgggaat tcctaccgga gtccgtactg cctgttgact
    19561 gatctttccg tttccccaaa tcgacagaga cagcccacca tgaaagccat gcagatcgtg
    19621 atacacaacg catagtgatg gtagagactg cggctccttt gtcagacgat ggagtccatc
    19681 ccgaacccgc tccggatcag gagccaggga tgcagacgga agccgtgaca gaagcacgta
    19741 tcccatccca caagcgacca caattacaag tccgacaacc aatgacagcg tcatctgcgc
    19801 cctctgctcc gcacagtcag gcaagtccac agccaggata cgcgcctggg gcacgaacgc
    19861 aaagcgatcc gtcgaagtgt tccggcttga tcaggacccg actccttttt caaagcacac
    19921 agggagtccc tcatcgctgt gccgcgagac gagaccgccg tggtatgatg gttggagtat
    19981 cttctgttca ttggggggac tcatgcgtac tgttcgccag cggaatactc gtggagcaac
    20041 ttggtctcta gtgattggag ggctcgccct cggcagcgtg gctgcgtcag caatcctcgt
    20101 ggcttgtggt tcatcgggaa acagcggcac cgatccttct ttggagtcgg cggatgccag
    20161 cgtgaacgat ccaggcggtg gggacggcgg caatggggat ggcggcaatg gggggccagg
    20221 aggaaaccaa gacggaggca tcgacggtgg tggcacagat ggaccgactc ccgatctctc
    20281 tcctctccca ccggacatga cgtgtgttcc tgctgcgaca ccagacgtac cagaaccgaa
    20341 gttcatggat acaaactgcg atggcattga tggtgatgca tcagcagcaa ttttcgtggc
    20401 gacgacggga aaggacacca atccagggac gatggcaagt cctgtactca cgatcaccaa
    20461 agcgctcgcg ctggcggcgg cccagagcaa gaccagtgtc tacgtctcaa aaggcaccta
    20521 catagagtcg gtgacgctga agagtgggat cagcctgtac ggcggctttg atgaaagcat
    20581 gggttggcag cgaagtgctg cgaacgtgtc gcgcattcag tggagcggca cggcggtgtc
    20641 tgcaacagga atctccgcgg aaacacacgt tgagtcactg acgatcagtg cacttcagcc
    20701 attgagcgga tattccacat acggcgtgct ggttgcaaat agcacgggcc ctgtcttcct
    20761 ccgctatgac cagatcaccg cgggagacgc tcctgcgggc ctgcctggca ccgcggggac
    20821 cgcaggggcg acaggtccaa atggtgcaaa tggcgcagcc gggatcatgg gagggatggg
    20881 accgactctc ggtgggtctg gcggtgtttc gtcttgtgga agaagcggcg gcaccggcgg
    20941 cattggcagt tccaaaaatg ggcagaatgg cgggcctggt actgggggag gagcggtcgc
    21001 gacgggagga gccggcggca gcggtggggc aggcacaacg tcgtgcctct tcatgatgag
    21061 cgtcgcagga ggcaatggat ccagtgggaa cgctggacct tcgggcacaa atgggaacgc
    21121 ggcggcctct ttttctggtg gaacagcaac gggaattccc ttgaattctg ggggaggagg
    21181 ggcagacggc acttccggag gcgcgggcgg cggcggcggc gcgggtggcg gcgattcggg
    21241 cggggtgtta tgtacggcgg acgcgggtgg tgccggtggt ggcggcggcg cgggtggctg
    21301 cggcgcaacc gggggaaaag gcggcggtgg cggcgggtca tccgttgggg tctttttgtt
    21361 ccagagcacg gtcacactga ccgactcgac gatcagttcg ggaaatggtg gtgctggagg
    21421 caaaggcgga aacggagcag ttgggggagc gggtggcccc ggtggaactg gcggcggaaa
    21481 cgcaggagag gcctctgctg gaggttctgg aggttccgga gcgcggggcg gggacagcgg
    21541 tgcgggggca ggcggcaatg gtggtgcaag ctttgcggta tacggcaaag gggcaacgct
    21601 ttcgcagacc aacaatacga tgaccaacgg tgcaggtggc gtcggcgggg ctggtggaac
    21661 gagcagcgca ggagcggcac caagcggcgt cagtggcccg tcgatggcca tccgtcttga
    21721 gtaggagcac tgcaccgccc ctctgtttgc gtgtgtatct agcggtgagg cggagccatt
    21781 caatcccgac cgcgtgccgc caggatgatg agccaaaaca tcagcgcgac ttcaccgatg
    21841 aaagtgaccg tggccatgcg cgtgccgtgg accagcagca accgggtcgc ctcgtcgatg
    21901 acataaccaa gaccacagac cgccaccgca gccgcgagcc cccgacgggc aactggactt
    21961 gcaaatcgtg tcacctccac cggccaagcg agcacgatta ggtggattcc gaacagaccg
    22021 atggccggcg caaacacctg ctgaaatgcc agcgcccgcg aagggccgtc tcctgcggta
    22081 gtggcaagtc gaaagaccgc ccaagcaagc agcgccgcat agatcacacg gagcccagcg
    22141 gccacggctg cgcttcgtcg tgcatcgatg tgaaacagac cccacacacc gattccgacc
    22201 acaacatcca aaaatgcaac gattcccatt gccagcgtcg ccaagtcgat ctgatgtggc
    22261 gtcgcctgcg cagcgcgttc gatgactgcg aagtttgcgt aaccggccag aactgccatc
    22321 accagcaaac caatcccagc aacggcagca gacaacctct gtgtgcgcgt catgcatcat
    22381 ccttctgcga aaaagatccg ccaccaagaa gccaacagcc aaacaccagc tcgaacagac
    22441 cgcccggaat cgaagaccag atcgccaccg gcagacctga aagcccgagc agagaaccca
    22501 gtgcaagcgc gctgtaggaa aacaccgccc accccgcgag ccagcgaggc aggagccgct
    22561 ccgtaagaag cgacagaaac agcggaacac tcccgaaacc aagtcccacc atcgcgacat
    22621 ccagtgccag cgtgctccca agcggcagcg ctttggcgcg aatcagaagg cccccgatgg
    22681 ccaaaatgat cgcctctgct gatcgagcaa ccagataccc gttggccgct tgcggaatcc
    22741 gcgcatggac gacggtgtgt agaagcacac cgatggccac cacgcccagg gaattgagca
    22801 ccacgagcac aagcccccat ggacgtccag ccagcgccgt accaccgcca tacaaaacaa
    22861 acgcagacag gaaaagtgcc ccgatcatgc gcgccactgc tttggttccc atgtgtcctc
    22921 tg
//
LOCUS       DAORGN010000065        22857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_886_length_22857_cov_6.057188, whole genome shotgun sequence.
ACCESSION   DAORGN010000065 DAORGN010000000
VERSION     DAORGN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_886_length_22857_cov_6.057188"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKL17_09340"
     CDS             <1..391
                     /locus_tag="PKL17_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44974.1"
                     /translation="DDRAYFQARVRDILQEAATIVGRPKALALAAQPETDTEICDGYV
                     GRGYALSTPQELSLLISVARTSGLLLDPVYTGKAFFGMVSELRKNPDCFGSRIVFLHS
                     GGLFGLLPLGDELLPVLATEQDTSAHD"
     gene            384..3212
                     /locus_tag="PKL17_09345"
     CDS             384..3212
                     /locus_tag="PKL17_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNK44975.1"
                     /translation="MTDSLWGRSSRPIFSTLRYASQGLFAGILLAAVELWFFRAPLVT
                     EFPLRTVQFRLLGHVVALTLLLFLPLGIVAGWLSRLSLWLDSQGKQKWSGLLWAGLCS
                     PLFFWISFSLFLGGKMQKIPFHPLWSTVLFGLSLIFTATFVPRFLHQVRKLDAQPIHK
                     RLVIALCLFGGLCALHLADLRILPRLYPWFHGSLLVGKMVIAGLIAPLVWFRLSRHFA
                     LHAAMFLLCGLTVWQGIRSIDKLRRAMALRTTVIEHTILPSRFLSLIPHRQMGRKAQA
                     IVDAQTQAPSYHGPRLAGRDVFLITIDALRHDEVSPNVTPTLHRLRQQGISFSRAYTQ
                     VPHTSFAVATLLTGKPVYALLQLGHDAGGHKTLPRILQNYRYKTAAFYPPSVFFVEHD
                     RLQALEESAYGFEYVKVEYLSGDRRTTQVIDFLETEKPQQVFAWIHYLEPHEPYDVHP
                     GGPDKSRPDRERYDGEVRFVDAEVARLVSYLRVHRPGAVLIVAADHGEEFGEHGGRYH
                     GTTLYDEQARVPLFFVDTAEHPIVQNREYAQPVGLIDVGPTLLGLLDIEPPQQMRGLD
                     LAPWIFTAQKSLPTRAIWSEIGKRKMVVYGSHKLICDLASDSCQVFDLLSDPQEQKNL
                     VDVDPRRTSELRGRLLGLLEESRRFEEAAANPHNNPQSETQKKAQNVQADLSRARLGD
                     RTVVPKLLSLLTDGSQAPIVTQQAVMLSAQLLSQSIPILQEDVDPVSALPPVLVRDAI
                     GALRRQLLSETEVKDRRWAAILLTRLGEQNRETDDIVQSLIGDEKAPAQQRLCAALSR
                     QMKPSCRGREKRDCIADALRVLDAALAIDDPDQVRPLLRVLGESRDGRALAPLVRNLS
                     TVRSRVDVVSALGSLADRRAVSALSQTLLSDPYVHVRAEAARSLAHIGGSEAHSFLQK
                     ALQTEREEAVLRTLRSAEQEFQSPTKTP"
     gene            3240..4346
                     /locus_tag="PKL17_09350"
     CDS             3240..4346
                     /locus_tag="PKL17_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNK44976.1"
                     /translation="MKYVRMPIEIESPEQLGYDNVKYNLTESSFADARLGDLDLDLRK
                     LLLCYGDHQGNRALRELIAAESGVSADDVLMTAGAAAALFVIATSLLSAGDHMIVERP
                     NYATNIETPRAIGAQIDFLELRFEDGFRPDIAQLQKLLRPQTKLVSITVPHNPTGSMI
                     SEAELLQIIGLCESKGITLLVDETYRDMAFGGPLKVAASLSDSVISVSSLSKTWGLPG
                     IRIGWLVCRDKKKMETLLAAKEQIFITNSVVDEEIALRFLLRKSEFAPAIFDTMKRNF
                     SVLSDWMANQDALEWVVPQGGVVCFPRFADSHPVDEDRFYRILNEQYQTYVGPGHWFE
                     MPRRYMRIGYGWPKTADLAHGLSAISAAAKAARK"
     gene            complement(4353..6548)
                     /locus_tag="PKL17_09355"
     CDS             complement(4353..6548)
                     /locus_tag="PKL17_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK44977.1"
                     /translation="MPAATGISSISALLAALCAVISGAVLLSGRRRPSHRTFLYFSTS
                     LFLWHLFSVFHSLTAPLALAALLFVPVTLLLFLRTWLRESVSSVELPWRGGPKTVWAL
                     LIAGEILLAYSYSVSGDPKRAWAAQLPPLLKGLVIPSLYLALTPLWQVYRQTVSRVDK
                     RRLLYLIGVGLLSITVTVWEHLPHPIGRGGANLGTVLSIVYLYFLHQTLFLDRLLDIN
                     ELLGRVVVLSVFVLLLSALHMLLTSLLHIEANQVVTVMVFVMLILYEPLRRFLEAQVH
                     RLTTHERQEQMQRLAELKSTLPNVIEPREAVRAVLSVFEETRRFTQAALYLLESDGNG
                     FELVGHFGSRPVDRLDAAARRPFLLRLVSTRQPVTLEQLEREHDLLTGRRGAVADLES
                     LDAIARTLVELQAGAVIGIYPTRNSDSSRRASTVSGAILRVSEETSFVPSEQLIGLLC
                     IKDDRSHDAFASAELDVVSAIAAQLGITVQNSQLYERMKERDRLAALGQMAAGLAHEI
                     RNPLGAIKGAAEFIAPSADGRLPEDAGDFVKIVIEETRRLNRVVSQFLDYARPWRGDE
                     LPLSVSDVIEKTVVLLRQILDEQSASLPDGVVLPKVQLELQPDLPVLRGDAEQLRQVF
                     LNLALNAVQAMADKPDGVLTISTGLRQGVRLGSAQQSIEIRFQDRGTGIDPQAMKNLF
                     IPFFTTKERGTGLGLPISQRIVENHGGFIEARNRPGGGATFIVVLPCSS"
     gene            complement(6599..7456)
                     /locus_tag="PKL17_09360"
     CDS             complement(6599..7456)
                     /locus_tag="PKL17_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNK44978.1"
                     /translation="MSHPPSDKPVETPSAEPAKEVSYSEILYEVKAGVAQITLNRPER
                     RNPIGASTVGELLHALERAKLDTAVKVVVLTGAGKVFSAGGDLSQMGGGGNPLQAGPS
                     ENRRAGTFVDLNLMLTGQGIGKPTIAKVNGHAMAGGLGLLVACDVAIAADDAQFATPE
                     INVGLWPMMIMSTIFRNVPRKRAMELILTGDRIDAATAEKIGLITRAVPRDRLDAEVQ
                     ALCDKICQKSPLVMRLGLDAFHKMQDMDLEPALRLLESELINVLGTEDAGEGLMAFLQ
                     KRAPVWKGR"
     gene            7604..8740
                     /locus_tag="PKL17_09365"
     CDS             7604..8740
                     /locus_tag="PKL17_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44979.1"
                     /translation="MRNLYRSPKVIVAGALALGMLVSGGTAKASCPTQSPEVSNDLSV
                     ECLIAPRKPVSGQSYDPTLYEQSMYRSLLSELTAVMAVPALDPADTVGYSGFHFTFDV
                     ATTSIKKDAPYWGGFAQPDGTKTQAGVRHVSGGFLPVASLMIRKGIWPLMLPIGFELG
                     FGGSNLLQSGIYALNGYAKIALHEGYHDIPVPSIAARATVSRLAGTPQVDMTILTAEG
                     LMSKAFGIGGTFTMEPYIGGGAIWSIVRSQVVDTAPNIDLYRGQPPGMTTVSAADSLA
                     QKVVFPTQDDIFRWRVFGGINFHYAIISVTLSYAFVGAGADNGFDKNGDGNFGPSDFP
                     TKIGAAPSGSAAACRSDASDGSKVCPKDVSGAQHTIGAGIGLRF"
     gene            complement(8819..9724)
                     /gene="tatC"
                     /locus_tag="PKL17_09370"
     CDS             complement(8819..9724)
                     /gene="tatC"
                     /locus_tag="PKL17_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNK44980.1"
                     /translation="MSSSEDPTVVQPTHHDEQRMPFLEHLAELRDRLRNAVLAIILAA
                     VGCYLVRGVLFQLLARPLLTAFRVAKGKGVAGQLIFTSPVEAFLVLLKTALVSSIFVA
                     SPVIFHQLWAFIAPGLYPHEKRWALPFVVVAVLLFVGGGLFCYYFVLPPGYEFFLTAA
                     TDASVELMKNIGSDIQIQDAVQIRPLISMEEYFGLTLMLLLVFGVVFELPLVLSVLAM
                     LGIVSAKSLWKFNRYAILIFAIAGAVLTPGDLVIGQLAMAGSLTVLYNLSIAIAWLVE
                     RKRSEAEVAETADDEKADAKAEPKE"
     gene            complement(9721..10200)
                     /locus_tag="PKL17_09375"
     CDS             complement(9721..10200)
                     /locus_tag="PKL17_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNK44981.1"
                     /translation="MFGLGPTELIVILVLGLLVLGPERIPGVASSLGKAIRSFRRATR
                     DLRDQIDLEDDVRRPFEDLRAALRDEPPAPNFPPPQIAPPLDPPLSVPVSSESHVDAA
                     ADIAAPIATLAPGVVSLVTQPAEPILTLAPPGSDSAPTPAETPPADATVQVPVEVSK"
     gene            complement(10235..11668)
                     /locus_tag="PKL17_09380"
     CDS             complement(10235..11668)
                     /locus_tag="PKL17_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNK44982.1"
                     /translation="MAEAIGIGMTASQSDADRFTGSPVQDAAALMTSALRRRRAALAK
                     TLSTACGGKPLLCRAGRPPSRNYPDNVYEFRASSHFLYLAGLPLEGAALLLDGDRSTL
                     FVHRTTEADALWHGETASPDALRQATGVDAVRYLDELQAFCQRLPPPLELPLRGSVAD
                     SPELEALVDAMIELRLRHDEHAISEIRRAAAVTVRAHRAGMAATSPGVRVSVVRAAME
                     REILAENFTTAYGSIVTTHGEVLHTLDRSSVCGQNDLLLADVGAESDLGFASDVTRTW
                     PTSGRFSASQRAMYDLVLAAQTTAIAAVRPGVRYRDVHLAACRVLLRGLIDHGLFVGE
                     LDGLIERGAHALFFPHGIGHLLGLDVHDMEDFGDRAGYAPGRTRASQFGLCYLRLDRD
                     LAPGNAVTIEPGLYFVPAILRSDRLVAPLRGDFRPDKLAQFADVRGIRIEDDVLVTAT
                     GHEVLTAGLGKGAAEVEAMCGNSSSAC"
     gene            11731..14307
                     /locus_tag="PKL17_09385"
     CDS             11731..14307
                     /locus_tag="PKL17_09385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNK44983.1"
                     /translation="MSVRAFRLPTHVRPLSYHIDLSTDPSRVEFAGTVTVELEITAQT
                     ETIILHARHLEVTRAEISAMFGETDSALITYQPEQETVTLLLGRPLPVGRARALLVFS
                     GRLAPSMHGLYLGSDGVHSAVCSQCEATDARAIFPCFDEPEFKAALRWTLRTGPGVTA
                     LSNGMLQQHHASHGEQVFEFAETKPVSSYLAAVVVGDFESDATDYVRDIPMKVYAPAG
                     KAKQTQFAKGFTSRLLPWFEQYFDFPYPFGKYDQVAVPGFDAGAMENIGLVLFRQNLL
                     LMEPGSASWRQEKIIALVIAHEMAHMWFGNVVTMRWWDDLWLNEAFAEWMAHKVCHAI
                     EPSYEVWNDFIEDKIRVLYDDALHSTHPIWTPVETPDQALEMFDSITYQKGCAVMRML
                     EHFLGEDSFRSGLRAYMKEFQYGNACGPDLWRALEKASSLPVHSLMQSWVTQSGYPVV
                     DLDLVERDDGAVLSLRQERFFSDAQSRPSDQLWAIPMTVRYADDEGVKTHRFIFDSRA
                     QTVKLATTGNVRFVYANQDEIGFYRQRMTQETLAKLLPIAVSVLSPVELVGLLEDNWA
                     MVRNGSISITSFLAVLSACSAARDHNVLRVIADRIDGLDLLVKDAGDSSARQKLRSLA
                     EQRFAPHLTELGFVPRAGEPQNDVQRRAICITTLASTARVQSVIREVELLAQAEMVNP
                     RAVDPNLAGPFVAIAAKFGDEPRYDRWVETFQTRKAAGKTPQEVARYLHTLSHFRRPE
                     LVQRTLQLIEQGMIPQEAVAAILAQLLSFRHSQLSAWEFLKQHWGTLRERFGDMGVSR
                     VVDALGRLRQSERADVVRFFEENKPAGAERALKRALERIDQGEELRQRVTRELLSYLL
                     AL"
     gene            14366..15931
                     /locus_tag="PKL17_09390"
     CDS             14366..15931
                     /locus_tag="PKL17_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44984.1"
                     /translation="MGISSSDSAPSPGEPTPSPNDPRPAPETAAGAKADPKTEPPTGA
                     APPAAPAATAATPSSPPATAAPAAPRSHRQRSALARFVSENTNVVSSFVIGIAGLAVT
                     TTYQCNQGELQKRQAEAQMRIAREQADNSWRIERAKILSENLKTLTARGGDTVEQRYG
                     VLLSLLRGSILEPDVAVSYALELGRDNPDYMRSVLTNIDRKDEQYYRRLETAYVLTCE
                     QRYGVSSQQVAQCASDKWAERSAALANTVLDNLEDLSAIDGPNSPMCLLHDEREVYPR
                     MLRLIGLFTPFLHDLYERRMWGPIDKFLAKSAGAKLVGTMALALASNDFSTETDDDRA
                     IRQRIEAVTQGYLAGTACDAECRLRVFAFALSNLSKSRGIFERVLRSQLLKPRPDIDP
                     LLNRLSTRIQFCQYDPNDTTIIRDGILQPVIEAEAFQAKPDLVKLELLLSMLADLPEP
                     TAPLSPAWQKLVDQLGKMSQGRYPKQLADRRADVQKQRRAMIGPPKPTEAGKKRPNFC
                     MATDDRAASDDEE"
     gene            16041..16937
                     /locus_tag="PKL17_09395"
     CDS             16041..16937
                     /locus_tag="PKL17_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HNK44985.1"
                     /translation="MQTESGLPQDIKAQLAPIPGGNPRTGVAGQVRFEYLGKPTELIG
                     SGIVAMLLLMVTCGLAYPWIIAGAQRYYYSRIRIHTDSGVLTPEFTGTGFLCFVEVFL
                     SSILVILTLGIYTPWYLANVLRYFMNNSQAVASDGTRYRLDYSGKGGELFVPMLVNML
                     LTNITLGIYGAWATCDLQKRIFEKTRILKGEHEIGKLDFVGTGGGLVGTLLLGMLLSM
                     VTLGLYLPWFQCNMEKFFRRNTAVYIGERRYRGDFSGEGGELFLLMLQVILVPLTLGL
                     YLFWLITKQNRFFIEKVSFQAE"
     gene            17125..18615
                     /gene="rpoN"
                     /locus_tag="PKL17_09400"
     CDS             17125..18615
                     /gene="rpoN"
                     /locus_tag="PKL17_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNK44986.1"
                     /translation="MSMKMEQSIRQQMSMVMTPQLQMAIKLLQMARAELVEQVQEELK
                     ENPLLEDAIDSDAEHERDEPTDHIETIGETEEPIAQPNAKEQTDAGKEVPVDGQPTNE
                     IDWATFIENSATAPLPAAQERPDNDDLPQAGQAPRTGESLEAHLLEQLRMARLPKDEE
                     RCAIFIIGNLDPDGYLKEPPLAEVADECEVSLEVAERALKRVQRFDPWGCGARTLQEC
                     LLVQAEMLDIDDKLMVTVITEHLVDIEKKNWEKLAKKLKQDVEEILLIAQEIQKLDPR
                     PGREFSTDQPQYITPDVYIHKVGEKYFVSVNDDGLPKLRISRYYLDTMKGKDNKAARD
                     YVQERLRSAKWLIDSIQQRQRTIVKVTESILKFQRDFFEKGAGHLRPLILRDVASDIG
                     MHESTISRATANKYVHTPQGLYELKYFFNIGINREGGQDEIASEAVKGFIKQLVSGEN
                     GKNPYSDQKIVELLKQKHGIEIARRTVAKYREQLGILPSSQRKSFS"
     gene            18778..19383
                     /gene="raiA"
                     /locus_tag="PKL17_09405"
     CDS             18778..19383
                     /gene="raiA"
                     /locus_tag="PKL17_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNK44987.1"
                     /translation="MQVAFTFRNVESSDTLKEYAKEKLERINKYFPDPIKAHIVFAQQ
                     RGYLFDVDVQITLHNGLVVKGDEATEDMHSSIDLVMAKIERQLRRFKDRIRDHKPQQG
                     PARQFMHRVVALEPQPAEIETAKPSPKAPEAPSQKSVPRVIREQKFVAVPMTVEEAIM
                     RMNLQHDPFLVFNNALTHEINVVYRRSDDTYGLIETTNDEK"
     gene            19422..19889
                     /locus_tag="PKL17_09410"
     CDS             19422..19889
                     /locus_tag="PKL17_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNK44988.1"
                     /translation="MKLVDLIRPELVIPELAGRSKAQVLAEFSRHLAKTQPGLDAALL
                     NRILTEREQLATTAIGEDIAIPHGKIEGATRMIGCVGRSTPGIAFDSLDGKPTHLFFV
                     LVAPESSAGLHLKALARISRVFKDAEFRAKLLRAPTAGEIFRIIAEEEQKYPS"
     gene            19889..20857
                     /gene="hprK"
                     /locus_tag="PKL17_09415"
     CDS             19889..20857
                     /gene="hprK"
                     /locus_tag="PKL17_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNK44989.1"
                     /translation="MPGVAVHSLLDVESGLALSVLGGKAGLFRKVGGSRIQKQGLALT
                     GYVQHVHPERMQVLGLTEVSFFRSLDDDARKRGAQSLASVRPCCVVITRGLEAPSELV
                     TSCEEQGVPLLTTPLMSSLFINRVTRLLENRLAPGTTIHGVLVDVLGVGVLLLGKSGV
                     GKSEAALDLVMRGHRLVADDVVEIRKRMPDLVIGSCSDILKHHMEVRGLGILSIKDLF
                     GIASVRDAKKVDLVIELSDWNPDDEYDRLGIDDLRFAILDVALPLLKIPVRPGRNIAA
                     IIEVAARNQLLKQQGIHSAREFQDRLTAAMQQTRPHSGMATPEDIE"
     gene            20910..21758
                     /gene="rapZ"
                     /locus_tag="PKL17_09420"
     CDS             20910..21758
                     /gene="rapZ"
                     /locus_tag="PKL17_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNK44990.1"
                     /translation="MHILVVTGVSGGGKSTALRALEDIGYYCVDNLPLPLLPQFVQLV
                     GNTGQKKAALVIDAREGEFLGGFRHEMRELRSKGLALEILFLDAPDDVLLRRFSETRR
                     RHPLHGDDLRAAIAHERKELLPLREEAHTVVDTGALNVHQLKGVIGERYGKSGQVLAL
                     TLLSFGFKYGLPVEADMVLDVRFLPNPYFIPALSNQTGLEPEVSDYVLSQPDAQTFVA
                     KAEALIEFYLPRAQREGKSYVTVAIGCTGGRHRSVAVVADLFNRLGARYQITVRHREL
                     GRGREP"
     gene            21755..22153
                     /locus_tag="PKL17_09425"
     CDS             21755..22153
                     /locus_tag="PKL17_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNK44991.1"
                     /translation="MNAPFTAVVVVTHGRAGADMLDAAQAFLHMQLPLVAAVGVNPDD
                     DQSVVNAKIDQALTDLGIDGWEDVLFLVDLVGSTPARLCCGKCGDKGHVVTGLNMPML
                     VKLATADRSTGPAALGSALVLTGTKSIHVE"
     gene            22162..22428
                     /locus_tag="PKL17_09430"
     CDS             22162..22428
                     /locus_tag="PKL17_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNK44992.1"
                     /translation="MLSAKLKIVNKAGLHARASTRFVQAANKYRSAVSVKKDGQEVNG
                     KSIMGVLLLVASIGSEITVSCDGDDAKECLQTLTELVNSGFGEN"
     gene            22437..>22857
                     /locus_tag="PKL17_09435"
     CDS             22437..>22857
                     /locus_tag="PKL17_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNK44993.1"
                     /translation="MGLGDESGPYPALREPIRPLAEEVRTGIAAAAGIAVGQAFVIDR
                     RRQPTPRRHLLPEEITAELVRLDEAVSLADMQLERLKRKLKESDESDSSVILEAHQLI
                     LRDEHLLGPTRRRIRDEKLNVEWALRKTVAEIQQRFDA"
BASE COUNT         4785 a         6976 c         6726 g         4370 t
ORIGIN      
        1 tgatgatcgc gcgtattttc aggcgcgcgt gcgcgacatt ctgcaagaag cggccacgat
       61 cgtgggtcgg cccaaagcac tggcgctggc cgcccagccc gaaacagaca ccgaaatctg
      121 cgatggctat gttgggcgcg gctatgcgct gtcgacgccg caagagctgt cgcttctgat
      181 ttcggtggcc cgcacatccg gtcttttgtt ggaccctgtc tataccggca aagcgttttt
      241 cggcatggtg tccgagctgc gcaaaaatcc cgactgtttc ggatcgcgga ttgtcttttt
      301 gcacagtggc ggtctgtttg gtctcttgcc gctcggggat gaacttctgc ccgtgctggc
      361 gacggaacag gatacgtctg cgcatgactg attcgctgtg gggcagatcc agccggccca
      421 ttttttcgac gctgcgctac gcaagccaag gactctttgc cggaatcctt ttggcagcgg
      481 tggagctgtg gtttttccgc gcgccgctgg tcacggaatt cccgctgcgc accgttcagt
      541 ttcggctttt ggggcatgtc gttgcgctga ctttgctttt gtttctgccg ctcggcatcg
      601 tggctggctg gctgtcgcgg ctgtcgcttt ggctggattc ccaaggcaaa caaaagtggt
      661 caggacttct gtgggccgga ctctgttcgc cccttttctt ctggatttcg ttttcgctgt
      721 ttctgggcgg aaagatgcag aagattccct ttcatccact ctggtcgacg gtgctgtttg
      781 gacttagtct gattttcact gctacttttg ttccccgctt cctgcaccag gtgcgcaaac
      841 ttgatgccca accgatccac aaacggctgg tcattgcgct gtgtctgttt ggcggactgt
      901 gcgcactgca ccttgctgat ctgcgcattt tgccccggct gtatccgtgg tttcatggca
      961 gtttgctcgt cggaaaaatg gtgattgcgg ggcttattgc accgcttgtg tggttccgtt
     1021 tgtccagaca ttttgcgctg catgccgcga tgtttttgct gtgtgggctg acggtttggc
     1081 aaggaatccg atcgatcgac aagctgcggc gggcgatggc gctgcgcacc acggtgatcg
     1141 aacacacgat cttgccaagc cgcttccttt cgcttatccc ccatcgccag atgggccgca
     1201 aagcccaggc cattgtcgac gcacaaaccc aagcaccgag ctatcacgga ccgcgcttgg
     1261 cgggtcgcga tgtctttttg atcaccattg atgcacttcg ccacgatgaa gtaagtccaa
     1321 acgtaacccc caccctgcat cggctgcggc agcagggaat ttcgttttcg cgcgcgtaca
     1381 cccaagtgcc gcatacgtcg ttcgcagtgg cgacgctttt gaccggaaag ccggtctatg
     1441 cactgcttca gcttggccat gatgcgggcg gtcacaagac gctgccgcgg attttgcaaa
     1501 actaccgcta taagactgcg gcgttttatc ccccgtcggt gttcttcgtt gaacacgacc
     1561 gcttgcaagc gctggaagag tccgcgtatg gctttgagta cgtcaaagtc gaatacctgt
     1621 ccggcgatcg gcgcacaacg caagtgatcg attttctgga aaccgaaaaa ccgcagcagg
     1681 tgttcgcgtg gattcactat ttggaaccgc atgaacccta cgatgtgcat cctgggggac
     1741 cggacaaaag tcgtcccgat cgcgaacgct acgacggaga agtccgcttt gtcgatgcag
     1801 aggtggcgcg tcttgtaagc tatctgcgcg tccatcgtcc gggtgcggtc ctgattgtcg
     1861 cagccgatca cggagaggaa tttggcgagc acggcgggcg ttatcacggc acgacgctgt
     1921 atgatgaaca ggcgcgcgtc ccactgtttt tcgtcgatac agccgaacat ccaatcgtac
     1981 aaaacagaga gtatgcacag ccggtgggac tgattgatgt cggaccgact ctgcttggtc
     2041 ttttggacat tgaaccacca caacagatgc gcggactgga tctggcgcca tggattttca
     2101 ccgcgcagaa gtcactgccg acgcgcgcca tctggagcga gatcggcaag cgcaagatgg
     2161 tggtgtacgg cagtcacaag ctgatctgtg atctcgcaag tgatagctgt caggtgttcg
     2221 atttgctttc cgatccacag gaacagaaaa acctggtcga tgtcgatccg cgacggacaa
     2281 gtgagctgcg aggccgtctg ctcgggcttt tggaagagtc gcgccgcttt gaagaagcgg
     2341 cagcaaatcc ccacaacaat ccgcaaagtg aaacgcaaaa aaaggcccag aacgtacaag
     2401 cggatctgtc gcgggcgcgg cttggcgatc gcacggtggt gcccaagctg ctgtctctgc
     2461 tcaccgatgg gtcgcaggct ccgatcgtga cgcagcaagc ggtgatgttg tcggcgcagc
     2521 ttcttagtca gtcgattccc attctgcaag aagacgtcga tccggtttcc gcacttccgc
     2581 ccgtgcttgt gcgtgacgcg ataggcgcgt tgcgtcgtca gcttttgagt gaaacagagg
     2641 tcaaagacag gcgctgggca gcgattctgc tgacccgttt gggagagcag aatcgtgaga
     2701 cagacgacat cgtacagagc ctgattggcg acgaaaaagc cccggcgcag cagcgacttt
     2761 gtgccgcact gtcgcggcag atgaaaccgt cttgcagagg ccgtgaaaaa cgcgactgca
     2821 ttgccgatgc gctgcgcgtg ctggatgccg cgctggcgat agatgatcca gatcaggtac
     2881 gaccgctgct gcgggtgctg ggcgaaagcc gcgatggccg cgcgctggct ccgcttgtgc
     2941 gcaacttgtc gacggtgcgc agccgtgttg atgtggtttc cgcgctcggc agtctggcgg
     3001 atcgacgcgc ggtctcggcg ctgtcacaga cactgctttc cgatccctat gtccatgtgc
     3061 gggcagaagc cgcgcgatca cttgcccaca ttggcggcag tgaagcgcat tcctttttgc
     3121 aaaaagcgct gcagacagaa cgggaagaag cggtccttag aacactccgt agcgccgaac
     3181 aggaattcca gtctccgacg aagacgccgt agtccttccc ttcccaaagg agtcctacca
     3241 tgaagtatgt tcgcatgccg attgagatcg aatcccccga gcagctcggc tatgacaacg
     3301 tgaaatacaa cctgaccgaa agttcatttg ccgatgcacg ccttggcgat cttgatctgg
     3361 atctgcgcaa gctgcttctg tgttacggag accatcaagg gaatcgggcg ctgcgcgagc
     3421 ttatcgccgc cgaaagtggc gtgtctgccg acgatgtctt gatgaccgcg ggcgcggcgg
     3481 cagcgctgtt tgtgattgcc acgtcgctgc tcagtgccgg cgatcacatg atcgtggaac
     3541 ggcccaatta cgcgaccaac atcgaaacgc cgcgcgcgat aggagcccag atcgattttc
     3601 tagagctgcg ctttgaagac ggatttcgtc ccgacattgc gcagcttcaa aaacttctgc
     3661 gtccacagac caaactggtc agcatcacgg ttccgcacaa tccgaccggc agcatgattt
     3721 cggaagcgga gcttttgcag atcatcgggc tgtgtgaaag caaaggaatc acgctgctcg
     3781 tcgacgaaac gtatcgagac atggcgtttg ggggaccgct gaaagtggcg gcatcgctgt
     3841 ccgattccgt catttcggtg tccagtctgt cgaagacctg gggactgccc ggaatccgca
     3901 tcggttggct tgtctgtcgt gacaaaaaaa agatggagac gctgctcgcg gccaaagaac
     3961 agatattcat cacaaactcg gttgtcgacg aagaaattgc gctgcgtttc cttttgcgca
     4021 agtcggagtt tgcccccgca atcttcgaca ccatgaagcg gaacttttct gttttgtcgg
     4081 attggatggc aaaccaagac gcgctggagt gggtcgtacc gcaaggcgga gtggtctgtt
     4141 ttccgcgttt tgccgattcc catcccgtcg acgaagaccg tttttatcga atcctcaacg
     4201 agcagtacca aacgtatgtc ggtcccggtc actggtttga aatgccgcgc cgctacatgc
     4261 gcatcggcta cggctggcca aagaccgccg atcttgcgca cggactttcg gccatcagcg
     4321 ccgccgcaaa agctgcccgc aagtaatccg cgttaggagg aacacggcag gacgacgatg
     4381 aaggtcgcgc cgccgccggg ccggttgcgc gcttcgatga agccgccgtg gttttcgacg
     4441 atgcgctggc tgatcggcag tccaagtccg gtgccacgtt ctttggtggt gaaaaaggga
     4501 atgaacagat ttttcatcgc ctgcggatcg attccggtgc cgcggtcttg aaaacgaatc
     4561 tcgatgctct gctgcgcact gccaagcctg acgccctggc gcagccccgt cgatatcgtc
     4621 agcaccccat ctggcttgtc cgccatcgct tgcaccgcgt tgagcgccag attcaaaaac
     4681 acctgacgaa gctgctcggc atcgccacgc agaaccggca agtcgggctg tagttcaagc
     4741 tgaaccttcg gcagcacgac accatcaggc agcgacgcgg actgctcgtc gagaatctgg
     4801 cgcaaaagca ccaccgtttt ttcgatcaca tcgctgacgc taagcggtag ttcatcgccg
     4861 cgccacggac gcgcatagtc gagaaactgc gaaacgacgc gattgagccg ccgcgtttcc
     4921 tcgatcacga tcttgacaaa atcacccgcg tcttcgggaa gtcgcccgtc agccgacgga
     4981 gcgatgaact ccgccgcacc ttttatcgcg ccgagcggat tgcgaatttc gtgggccaga
     5041 cccgccgcca tctgtccaag cgccgccagg cggtcgcgct ctttcattcg ctcgtagagc
     5101 tgcgagtttt gcaccgtgat gccaagctgc gccgcaatcg ccgacaccac atccagctct
     5161 gccgacgcaa acgcatcatg cgagcgatca tcctttatac aaagcaggcc gatcagctgc
     5221 tcgcttggca caaacgatgt ctcttcgctg acccgcagaa tcgccccgct caccgtcgaa
     5281 gcccggcgag aagagtcgct gttgcgggtc ggatagatgc caatcaccgc accggcttgc
     5341 agctcgacca gcgtgcgcgc aatcgcatcc agcgattcca gatccgccac cgcaccccgc
     5401 cgtcccgtca gcaaatcgtg ctcgcgttcg agctgttcca gcgtcaccgg ctgccgcgtc
     5461 gacaccaagc gtaacaaaaa gggccgccgc gctgccgcat cgagcctatc gacaggacgc
     5521 gagccaaagt gcccgaccag ttcaaagcca ttgccgtctg attcgagcag atagagcgcc
     5581 gcttgggtaa agcgccgcgt ttcctcgaac accgacagca ccgctcgcac cgcttcgcgt
     5641 ggctcgatca cgttcggcag ggtgcttttc agctcggcga gccgctgcat ctgttcctgc
     5701 ctttcgtgtg tggtcagtcg atgcacttgc gcttcgagaa agcggcgcag cggttcatac
     5761 agaatcagca tcacgaacac catcaccgtg acaacttgat tggcctcgat gtggagcagc
     5821 gacgtcaaaa gcatgtgcag cgccgacaaa agcagcacga acaccgacag caccacgacg
     5881 cgcccgagca gctcgttgat gtcgagcagc cgatccaaaa acagcgtctg gtgcaaaaag
     5941 tacagataga cgatagacag caccgtcccc aggtttgcgc cgccgcggcc aatcggatgc
     6001 ggcaggtgtt cccacaccgt gaccgtgatc gacaaaagcc cgacgccaat cagatagagc
     6061 agccgccgct tgtcgacccg cgacaccgtc tgccgataga cctgccacaa aggtgtcagg
     6121 gctagataca ggctcgggat gaccagccct ttgagcagcg gcggaagctg cgccgcccag
     6181 gcccgcttgg gatcgccgct caccgaatag gaataggcca gcaaaatttc ccccgcgatg
     6241 agcagcgccc agacggtttt gggcccacct cgccacggca gttcgacgct gctgacgctt
     6301 tcgcgcagcc aggtacgcag aaacaaaagc agcgtgaccg gaacgaacaa aagcgccgcc
     6361 aaagcgagcg gagcggtcag gctgtgaaag accgaaaaca ggtgccaaag aaacaggctg
     6421 gtcgaaaaat acagaaaggt gcgatgtgac ggacgtcgcc gccccgacag cagcaccgcg
     6481 cccgaaatga ccgcgcacag cgctgccaaa agtgccgata tcgaagaaat tcccgtcgcc
     6541 gcaggcacgg ttttatgtcc ttggtcgggt tactttgtgg cggcagaaag agcgtaagct
     6601 agcgaccctt ccacacgggc gcacgctttt gcaaaaacgc cataagccct tcgcccgcgt
     6661 cctcggtgcc cagcacgttg ataagctcgc tttcgagcag ccgcagcgcc ggttccagat
     6721 ccatgtcctg catcttgtgg aaggcgtcga gcccaagccg catcaccagc ggacttttct
     6781 gacagatttt gtcgcacagc gcctgcactt cggcatcgag ccggtcgcgc ggcaccgccc
     6841 gcgtaataag accaatcttt tccgccgtcg ctgcgtcgat gcgatccccg gtcaaaatca
     6901 gttccatggc gcgcttgcgt ggcacgttgc ggaaaatcgt cgacatgatc atcatcggcc
     6961 acagaccgac gttgatttcg ggggtggcaa actgcgcgtc gtcggcagca atcgccacat
     7021 cgcatgcgac aagaagccca agcccgcccg ccatcgcgtg gccgttgacc ttggcgatgg
     7081 tcggcttgcc gattccctgg ccggtcagca tcaggttcag gtcaacaaac gtccctgccc
     7141 gtcggttttc gctcggtccg gcttgaagcg gattgccgcc gccgcccatc tggcttaggt
     7201 caccgcccgc ggaaaaaacc ttgccggcgc cggtcagcac cacgactttg accgccgtat
     7261 ccagcttggc ccgctccagc gcatgcaaaa gctcgccgac cgtggatgcg ccgatgggat
     7321 tgcgccgctc cggtcgattg agcgtgatct gggcaacgcc cgctttgact tcgtacaaaa
     7381 tttcgctgta gctgacttct tttgcgggct ctgccgacgg agtttctact ggcttatccg
     7441 aaggtgggtg gctcatggcc gaactataca ccagggcaag caaactctcg tcgagacact
     7501 ttattttcgc gcccgccaag gtgccgcagc agcgtttgcg cccgtcgcga cgagagcgcc
     7561 aattgacaca ggaattttga aagtcgtaca ctcatttgcg agcatgcgta acctctatcg
     7621 ttctcccaag gtcatcgttg cgggcgcact tgcgctgggg atgcttgttt cgggcggcac
     7681 cgccaaggca agctgcccca cccagtctcc agaggtctcc aatgatctct ccgtcgagtg
     7741 cctgatcgcg ccacgcaagc ctgtgtcggg acagagctac gatccgacgc tctatgagca
     7801 gtcgatgtat cgctcgctgc tttccgagct gaccgcggtc atggccgtgc ctgcgctcga
     7861 ccccgcggac accgtgggct attcgggctt ccactttacg ttcgacgtgg ccaccacatc
     7921 gatcaaaaag gacgcgcctt actggggcgg ctttgcgcag cccgacggaa ccaagacgca
     7981 ggccggtgtc cgccacgtca gcggaggctt ccttcccgtc gcctcgctga tgattcgcaa
     8041 aggcatctgg ccgctgatgc tgcccatcgg tttcgagctg ggctttggcg gttcgaacct
     8101 gcttcagtcg ggaatttatg ccctaaacgg ctacgccaaa attgcgctgc acgaaggcta
     8161 ccacgacatt ccggtgccat cgatcgcagc gcgtgcgacg gtaagccgtc tggcaggaac
     8221 gccgcaggtc gacatgacca tcctcaccgc ggaaggcctg atgtccaaag cgtttggcat
     8281 cggcggcacc ttcacgatgg agccgtacat cggcggtggc gcaatctggt cgatcgtgcg
     8341 cagccaagtc gttgacaccg cgcccaacat cgacttgtac cgcggccagc cgccaggaat
     8401 gacgacggtt tcggcggcgg attcgctggc ccaaaaggtg gtcttcccga cgcaggacga
     8461 catcttccgc tggcgcgtgt ttggcggcat caacttccac tacgcgatca tctcggtcac
     8521 gctgtcgtat gcgttcgtcg gtgcgggcgc tgacaacggc ttcgacaaga acggcgacgg
     8581 caacttcgga ccaagcgact tcccgaccaa gatcggcgcc gcgccatcgg gttcagccgc
     8641 agcctgccga tccgatgcaa gcgacggcag caaagtctgc cccaaagatg tctcgggcgc
     8701 gcagcacacc atcggtgctg gcatcggact ccgcttctag tcgaatcgat gttcgcggaa
     8761 gcccgctcgc ttccgccccc gcctgacccc gccgccagcg ccccgcccca acatccaact
     8821 actctttcgg ttccgccttc gcgtcggcct tttcgtcgtc agccgtttcc gcaacttcgg
     8881 cttcgctgcg cttgcgttcc accagccagg caatcgcgat gctcaggttg taaagcaccg
     8941 tcagcgagcc cgccatcgca agctgcccga tcaccaagtc accgggggtc agcaccgcgc
     9001 cggcaatcgc aaaaatcagg atcgcgtagc gattgaactt ccacagagac ttcgccgaga
     9061 caatccccag catcgccagc accgacagga ccagcggcag ctcgaacacc acgccgaaca
     9121 ccaaaagcag catcagcgtc aggccgaagt actcttccat gctgatgaga ggccgaatct
     9181 gcaccgcgtc ttggatctga atgtcgctgc cgatgttctt cattagctcg acgctggcgt
     9241 cggtggccgc ggtcaaaaag aactcgtagc cgggcggcag cacgaagtag taacaaaaca
     9301 ggccgccacc gacgaacagc agcaccgcca ccaccacaaa cggcagggcc cagcgctttt
     9361 cgtgcggata cagacccggt gcgataaacg cccacaactg atgaaaaatg accggcgagg
     9421 cgacgaaaat cgacgacaca agcgcggtct tgagcagcac caaaaaggct tcgacaggac
     9481 ttgtgaagat gagctgaccg gcgacgcctt tgcctttggc aacccgaaac gctgtcaaaa
     9541 gaggccgcgc cagcagctga aagagcacgc cgcggaccag ataacagccc accgccgcca
     9601 agatgatcgc cagcaccgcg ttgcgcagcc tgtcgcgcag ctcggccagg tgttccaaaa
     9661 atggcatccg ctgctcatcg tggtgcgtag gctgcacgac ggtgggatct tcactgctgc
     9721 tcatttgctg acctcaaccg ggacctgaac cgtcgcatcc gcaggcggcg tttcggcggg
     9781 tgtcggggca gaatcactgc cgggcggggc caacgtcagg atcggttccg ccggctgtgt
     9841 cacaagcgag acaacgccag gggccagcgt cgcaatcggg gccgcaatgt ctgcggcagc
     9901 atcgacatgc gattcgctgg aaaccggcac cgacagcggt gggtcgagcg gcggcgcaat
     9961 ctgcggcggc ggaaaattcg gcgcgggtgg ctcgtcgcgc agcgcagcac gcaggtcttc
    10021 aaacggtcga cgcacgtcgt cttccagatc gatctggtcg cgcagatcgc gggtcgcgcg
    10081 tcggaagcta cgaatggctt ttccgaggct gctggcgacg ccgggaatcc gctctggccc
    10141 gagcacaagc agcccaagga ccaagatcac gataagttcg gtggggccga gtccaaacat
    10201 acgcgcagtc tagcaccacc gcttatgcgc cgtgtcagca agccgacgac gaattgccac
    10261 acatcgcttc gacctcggct gcgcccttgc ccagcccggc ggtcagcacc tcatgtcccg
    10321 tcgccgtcac cagcacatcg tcttcgatgc gaatgccgcg cacatcggca aactgcgcca
    10381 gcttgtcggg ccggaagtcc ccacgcagag gcgcaaccag ccgatcactg cgcagaatcg
    10441 ccggaacaaa gtacagcccc ggttcaatcg tcaccgcgtt gcccggtgcc aggtcgcgat
    10501 ccagccgcaa atagcacaga ccaaactggc tcgcgcgcgt tcgacccggc gcataaccgg
    10561 cgcgatcccc gaaatcttcc atgtcgtgga catccagccc gagcaagtgc ccgatgccgt
    10621 gcggaaaaaa cagcgcgtgc gccccgcgct cgatcagccc atccagctcg ccgacaaaca
    10681 gtccgtgatc gatcaggccg cgaagcagca cccgacacgc cgcaagatga acatcccgat
    10741 agcgaacccc aggccgcacc gccgcaatcg ccgtggtttg cgccgccaaa accaggtcgt
    10801 acatcgcacg ctggctggcc gaaaagcgac cgctcgttgg ccaagtccga gtcacgtcgc
    10861 tggcaaagcc caggtcactt tcggcgccga catcggccag cagcaaatcg ttctgtccac
    10921 agacagagct gcgatcgagg gtgtgcagca cttcgccgtg tgtggtgacg atgctgccat
    10981 atgcggtggt gaaattttcg gcaagaattt cgcgttccat cgcagcccgc acgacgctga
    11041 cccgcacacc gggcgaagtc gccgccatcc ctgcgcgatg cgcccgcacc gtcaccgccg
    11101 ccgcccgtcg gatttcggaa atcgcgtgct catcgtggcg cagccgaagt tcgatcatcg
    11161 cgtcgaccag cgcttcaagt tccggcgaat cagcaaccga accacgcagc ggcagttcca
    11221 gcgggggcgg cagtcgctga cagaacgctt gtagttcatc gagatagcgc accgcatcga
    11281 cgccagtcgc ctgacgaagc gcatctgggc tggcggtctc accatgccac agcgcatcgg
    11341 cctcggtggt ccgatgcaca aacagcgtcg aacgatcccc atcgagcagc agcgccgcgc
    11401 cttcgagcgg cagacccgca aggtacagaa aatgactgct cgcgcgaaac tcgtagacgt
    11461 tgtcgggata gtttctcgat ggtggacgcc cagcccggca cagcagcggc tttccgccac
    11521 acgccgtcga cagcgttttc gccaaagccg ctcgccgccg ccgtagcgcc gacgtcatca
    11581 gcgccgccgc atcctgcaca ggcgaacccg tgaagcgatc tgcatccgat tgagaagccg
    11641 tcatcccgat tccgattgcc tccgccatga cgcaaactgt acacaaaatt cgaggtagaa
    11701 aacgcaagct gcgctattgt cacaagtcct atgagcgtcc gcgcgttccg actcccgacc
    11761 cacgttcgtc cactttccta ccacatcgat ctcagcaccg acccaagccg cgtcgagttt
    11821 gccggcactg tcaccgtcga actggagatc actgcccaga cggaaacgat catcttgcac
    11881 gcccgtcatc tggaagtgac gcgggcggaa atcagcgcga tgttcggcga aaccgacagt
    11941 gccctgatca cgtaccagcc agagcaagag accgtgacgc tgctgcttgg tcggccactt
    12001 cccgtcgggc gcgcgcgggc actgctggta ttttcgggac gactggcgcc gagcatgcac
    12061 ggcctttacc ttggcagcga tggtgtacac agcgccgtct gttcgcagtg tgaagcaacc
    12121 gacgcccgcg cgattttccc gtgttttgac gaaccggagt tcaaagcggc gctgcgctgg
    12181 acgctgcgaa ccggccccgg tgtaacggcg ctgagcaacg gtatgctcca gcagcatcac
    12241 gccagccacg gcgagcaggt gtttgagttt gccgaaacca agccggtatc gtcgtacctg
    12301 gctgcggttg tcgtcggtga ctttgaatcg gatgccaccg attacgtccg cgacatcccg
    12361 atgaaggtct atgccccggc gggcaaggcc aaacaaacgc agtttgccaa aggctttacg
    12421 tcgcgacttt tgccgtggtt tgagcagtac ttcgacttcc cctacccgtt tggcaaatac
    12481 gaccaagttg cggtgccggg cttcgacgcg ggcgcgatgg aaaacatcgg cctggtgctg
    12541 ttccgtcaga acttgctgtt gatggaaccg ggcagcgcaa gctggcgaca ggaaaaaatc
    12601 attgcgctcg tcatcgccca cgagatggcg cacatgtggt ttggcaacgt cgtgacgatg
    12661 cgctggtggg atgatctgtg gctcaacgag gcgttcgccg agtggatggc ccacaaagtc
    12721 tgtcacgcga tcgagcccag ctacgaagtc tggaatgact ttatcgagga caaaattcgc
    12781 gtcctctacg acgatgcgct gcacagtacg catccgatct ggacccccgt cgagacgccc
    12841 gaccaagcgc tcgaaatgtt cgattcgatc acgtaccaaa agggctgcgc agtgatgcga
    12901 atgctcgaac acttcctcgg ggaagacagc ttccgcagcg gcctgcgcgc ctacatgaag
    12961 gaattccagt acggcaacgc ctgcggcccg gacctgtggc gtgcgctcga aaaggccagt
    13021 tcgctgccgg tccactcgct gatgcaaagc tgggtgacgc agtcgggcta cccggtggtc
    13081 gatctggatc tggtcgagcg ggatgacggc gcggtgctca gtctgcgcca agagcgtttc
    13141 ttttccgatg cccagagcag gccaagtgat cagctatggg cgattccgat gacggtgcgc
    13201 tatgccgacg acgaaggcgt caaaacccac cgcttcattt tcgattcgcg cgcacagacg
    13261 gtcaagctgg cgacgacggg gaatgttcgc ttcgtgtacg caaaccaaga cgaaattggt
    13321 ttctatcggc agcggatgac gcaagaaacg ctggccaagc tgctgccaat cgccgtctcg
    13381 gtgctgtcgc cggtcgagct ggtcggactg ctcgaagaca actgggcgat ggtgcgaaat
    13441 ggcagcattt cgatcaccag ctttttggcg gtgctgtcgg cgtgctcggc ggcgcgcgat
    13501 cacaacgtct tgcgcgtgat tgccgatcga atcgacgggc tcgatctgct ggtcaaagat
    13561 gccggcgatt catcggcgcg acagaagctg cgaagcttgg ccgagcagcg ctttgcgcca
    13621 cacctgaccg agctgggatt tgtaccgcgc gcgggcgagc cgcaaaacga cgttcagcga
    13681 cgagcgattt gcatcacaac gctggcatcg acggcacggg tacaaagtgt catccgcgaa
    13741 gtcgagcttc tggcgcaggc cgaaatggtc aatccgcggg cggtcgatcc caacctggcc
    13801 ggtccatttg tcgcgattgc ggccaagttt ggcgacgagc cacgctacga tcgctgggtc
    13861 gagacgttcc agacccgcaa ggccgcgggc aagaccccac aagaagtcgc gcgttacctg
    13921 cacacgctgt cgcatttccg tcgtccggag ctggtccagc gcacgctgca gctcattgaa
    13981 caaggcatga tcccgcagga agccgtcgcg gcgatcttgg cgcagcttct gtcgttccgg
    14041 cactcgcagc tctcggcctg ggaattcctc aagcagcact ggggtacgct gcgcgagcgc
    14101 tttggtgaca tgggcgtgtc acgagtcgtc gatgcactcg ggcggctgcg ccaaagcgaa
    14161 cgggccgacg tggtgcggtt cttcgaagag aacaagcccg ccggagccga acgtgcgctc
    14221 aagcgagcct tagagcgcat cgatcagggc gaagagctgc ggcagcgtgt cacccgtgag
    14281 ctgctaagct acctgctcgc gctgtagacg gtcgattcta ggcggcgctt gcgtctgccc
    14341 ggagcgctcc tagatactgg aagtaatggg catttccagc agcgattccg caccttcgcc
    14401 cggcgagcca accccttcac caaacgaccc gcgcccggcc cccgaaacag cagccggggc
    14461 caaagccgat ccgaagaccg aacctccgac tggcgccgca ccacctgcag cccccgcagc
    14521 aacagccgcc acgccgtcgt ctccacctgc gacagcggca ccagcagccc cgcgcagcca
    14581 tcgacaacgg agcgcgctgg cccggtttgt ttcggaaaac accaacgtcg tttcaagctt
    14641 tgtgatcggt atcgcaggac tcgcggtcac gacgacgtac cagtgcaacc aaggcgagct
    14701 gcaaaagaga caagccgaag cgcagatgcg gatcgcgcgc gaacaagccg acaacagctg
    14761 gcggatcgag cgagccaaga ttctgtcgga aaacctcaag acactgaccg ctcgtggcgg
    14821 tgataccgtc gagcagcgct atggcgttct gctttcgctt ttgcgtggca gcattctcga
    14881 accggacgtc gcggtgtctt atgcgctgga gctgggccgg gacaatcccg actacatgcg
    14941 cagcgtgctg accaacatcg accgcaaaga cgagcagtac tatcgacgac tggagacggc
    15001 atacgttctg acctgcgagc agcgctatgg cgtgtcatcg cagcaggtgg cgcagtgcgc
    15061 gagcgacaag tgggccgagc gatccgcggc gctggccaat acggtgctcg acaacctgga
    15121 agacctgagc gcaatcgacg gaccgaactc gccgatgtgc ctgctccacg acgagcgcga
    15181 ggtctacccg cgcatgctgc ggctgatcgg actttttacg ccgtttttgc acgacttgta
    15241 cgaacgacgg atgtgggggc cgatcgacaa attcctggcg aaaagtgcgg gagccaagct
    15301 ggtcggaacg atggcgctgg cgctcgcgag caacgatttt tcgacggaaa cggacgacga
    15361 tcgcgccatc cggcagcgga ttgaagccgt cacccaaggc tatcttgcgg gcaccgcctg
    15421 cgacgccgag tgtcggctgc gggtgttcgc tttcgcgctg agcaatctgt caaaaagtcg
    15481 cggtattttt gaacgggttc tgcgcagtca actgctcaag ccgcgtcccg atatcgatcc
    15541 gctgctcaat cggctgtcga cgcgaatcca gttttgtcag tacgacccaa acgacacgac
    15601 gatcatccgc gacggcatct tgcagcctgt catcgaagcg gaagcgttcc aagccaagcc
    15661 cgatttggtc aaactggagc tgcttttgtc catgctggcc gatctgcccg aaccgaccgc
    15721 gccgctcagc ccagcgtggc aaaagctcgt cgatcagctt ggcaaaatgt cgcaaggtcg
    15781 ctatcccaaa cagctcgccg accgtcgcgc cgacgtgcaa aaacagcgcc gcgccatgat
    15841 tggtccgccc aaacccaccg aagctggcaa aaagcgtccg aacttctgta tggcaaccga
    15901 tgaccgggcg gcaagcgacg acgaggaata gcggtccgtc cacaagctgc tttccctaga
    15961 ccacgcaccg ctggatactt atcccgatac agaaatcgcc aggcatgttt gctggcacca
    16021 catttcggga ggttcgtttt atgcagacgg agagtggtct tccacaagac atcaaagccc
    16081 agcttgcgcc gatccccggc ggaaacccgc ggaccggcgt cgctggccaa gttcgttttg
    16141 aatatctggg caagccgacg gagcttatcg gcagcggcat cgtcgcgatg ctcctgctga
    16201 tggtgacgtg cggcctggcg tatccgtgga ttatcgccgg ggcgcagcgc tactactatt
    16261 cgcgcattcg catccacacc gactcgggcg tgctgacccc cgaatttacc ggcaccggct
    16321 tcctctgctt cgtcgaggtg ttcctgtcgt cgatcctggt gatcctcacg cttggcattt
    16381 acacgccttg gtatctcgcc aacgtgctgc gctatttcat gaacaacagc caagccgtgg
    16441 ccagcgacgg cacgcgctac cgtttggatt acagcggcaa aggcggcgag ctgttcgtcc
    16501 cgatgctcgt gaacatgctt ttgaccaaca tcactctcgg catttatggc gcgtgggcga
    16561 cgtgcgacct gcaaaaacgc atctttgaaa agacccgcat cctcaaaggc gagcacgaga
    16621 tcggcaagct cgatttcgtc ggcactggcg gcggtctcgt cggaacgctt cttttgggca
    16681 tgctgctgtc gatggtgacg ctgggtcttt atctgccgtg gttccagtgc aacatggaaa
    16741 aattcttccg tcgcaacacc gcggtctaca tcggtgaacg acgctatcgc ggcgacttca
    16801 gcggcgaagg tggcgagctg tttttgctga tgctgcaagt gatcttggtg ccgctcacgc
    16861 tcgggctgta tctgttttgg cttatcacca agcagaaccg cttctttatc gaaaaggtgt
    16921 cgttccaagc cgagtaagca gtccgccgtc ccgcccttcg ttcccctctc ctgccagcct
    16981 cagtttttgc gtcacgtcaa gaccgtggcg caagcgcttg tctttgttcg catgcaagat
    17041 gtataccgac tatcaataat caagctagta ccgtgggttc tcccggtgta gcctggtagc
    17101 agaaggtcgg aggccgataa gcgcatgtcc atgaagatgg aacagagcat tcgacagcaa
    17161 atgtcgatgg tcatgacgcc ccagttgcag atggcgatca agcttctgca aatggcgcgg
    17221 gccgagctgg tcgaacaagt gcaagaggag ctcaaggaaa atccgctcct cgaggatgcg
    17281 atcgacagcg acgcggagca cgagcgcgac gagcccacag atcacatcga gacgatcggg
    17341 gagaccgaag agccgatcgc gcagccgaac gccaaagaac aaacggacgc cggaaaagaa
    17401 gttcccgtcg acggtcagcc aacaaatgag atcgactggg cgacttttat cgaaaactcg
    17461 gcgacggcac ctttgccggc agcgcaggaa cgacccgata acgatgatct gccgcaggcc
    17521 ggtcaggcac cgcgcaccgg cgagtcgctg gaagcccatc tgctagagca gcttcggatg
    17581 gcgcggctgc ccaaagacga ggaacgctgt gcgattttca tcatcggcaa cctcgatccc
    17641 gacggatatc tcaaggaacc accgctcgcc gaagtcgccg atgagtgcga ggtctctctc
    17701 gaagtcgcgg agcgggcact aaagcgggtg cagcgatttg atccgtgggg ctgcggcgca
    17761 cgcacgctgc aagagtgtct tttggtgcag gccgagatgc tcgacatcga cgacaagctg
    17821 atggtcacag tcatcaccga gcaccttgtc gacatcgaaa agaaaaactg ggaaaagctc
    17881 gccaaaaagc tcaagcaaga cgtcgaagaa atcctgctca tcgcgcagga gatccagaag
    17941 ctggaccctc gtcccggtcg cgaatttagc accgaccagc cgcagtacat cacgcccgat
    18001 gtctacatcc acaaggtcgg cgaaaagtac ttcgtttcgg tcaacgacga cggactgccg
    18061 aagctgcgca tcagccgcta ctacctggac acgatgaaag gcaaggacaa caaggcggcg
    18121 cgcgactacg tgcaagagcg gctccgatcg gccaagtggc ttattgacag catccagcag
    18181 cgtcagcgca cgatcgtcaa agtcacggag tcgatcctca agttccagcg cgacttcttc
    18241 gaaaagggcg ctgggcactt aaggccgctc atcttgcgcg acgtcgccag cgacatcgga
    18301 atgcacgaat cgacgatcag ccgcgcgacg gcaaacaagt acgtccacac cccgcaggga
    18361 ctttacgagc tgaaatactt tttcaacatc ggcatcaatc gcgagggtgg ccaagacgag
    18421 atcgcgtcgg aagcggtcaa aggcttcatc aagcagctcg tcagcggtga aaacggcaaa
    18481 aatccgtact cggatcagaa aatcgtcgag cttttgaagc aaaaacacgg catcgaaatc
    18541 gcgcggcgga cggtcgccaa ataccgcgag cagctcggga ttttgccgtc gagtcagcgc
    18601 aagtcgtttt cgtgatcccg gtgcttgcga agcgctgaaa ttgcgctaag aaatgctcaa
    18661 atagcttgtg aaagcgtttg gcggttgacc acaagagctc atcggtcgta gaagcgagcg
    18721 gcacaaaagc gatggcggtt tggccacgcc gctctcaaag gagaggaggg gagtgtcatg
    18781 caggttgcct tcacattccg gaacgtcgaa tccagcgata cgctcaagga gtacgccaag
    18841 gaaaagcttg agcgcataaa caagtacttc cccgatccaa tcaaggcgca catcgtcttt
    18901 gcgcagcagc gcggttatct gttcgacgtc gacgtccaga tcacgctgca caacggcctg
    18961 gtcgtaaaag gcgatgaagc gaccgaagac atgcactcgt cgatcgatct ggtgatggcc
    19021 aagatcgaac ggcagctgcg acggttcaaa gatcgcattc gcgatcacaa gccgcagcaa
    19081 ggtccggcgc gtcagtttat gcaccgcgtc gtcgcgcttg agccacagcc ggcagaaatc
    19141 gagacggcca agccttcgcc caaggcgccc gaagccccca gccagaagtc tgttccgcgg
    19201 gtgatccgag agcagaagtt cgtcgctgtt cccatgacgg tcgaagaggc tatcatgcgg
    19261 atgaacctgc aacacgatcc gttcctggtc tttaacaacg cgctcaccca tgagatcaac
    19321 gtggtctata ggcggagcga cgacacatac ggtctgatcg agaccacgaa cgacgaaaaa
    19381 tagggctttg gtggcgcgat gggcaaggca aactgcggac gatgaagctg gttgatctca
    19441 tcaggcctga gctggtcatt cccgagctgg cagggcgaag caaagcgcaa gtcttggcgg
    19501 agttttcccg tcacttggcg aagacccagc cggggctcga cgcagcgctc ctgaatcgca
    19561 tcctcaccga gcgagaacag ctagccacca ccgccatcgg cgaagacatc gcgattcccc
    19621 acggcaagat cgagggagcc acccggatga tcggctgtgt cggacgctcg acgccgggca
    19681 tcgcgtttga ctctctcgat ggcaagccca cgcacctgtt ttttgtcctc gtcgccccgg
    19741 aaagctcggc gggactgcat ctgaaagcgc tcgcgcgaat cagccgcgtg tttaaagatg
    19801 ccgagtttcg cgccaagctt ttgcgagccc ccaccgccgg cgaaattttc cgcatcatcg
    19861 ctgaggaaga gcaaaagtac ccaagctgat gccgggcgtt gctgtccata gcttgctcga
    19921 cgtcgagtcg ggactggcgc tctcggtgct cgggggcaag gcgggactgt ttcgcaaagt
    19981 agggggctcg cgcattcaaa aacaggggct ggcactcacc ggctatgtgc agcacgtcca
    20041 ccccgaacgg atgcaggtgc tcgggctcac cgaggtatcg ttctttcgca gtctcgacga
    20101 cgacgcccgc aagcgtgggg cccagtcgct ggccagcgtc agaccgtgct gcgtggtcat
    20161 cacccgcgga ctcgaggccc caagcgagct tgtgacttcg tgcgaagagc aaggcgttcc
    20221 gcttctgacg acgccgctta tgtcttcgct gtttatcaac cgagtgacgc ggcttttgga
    20281 aaatcggctc gcaccgggca cgacgattca tggcgttctc gtcgatgtgc tcggggtcgg
    20341 tgtgcttctg ctcggcaagt ccggcgtcgg caaaagcgaa gcggcgcttg atctggtgat
    20401 gcgcggccac cggctcgttg ctgatgatgt cgtcgaaatt cgcaaacgga tgcccgatct
    20461 ggtgatcggc tcgtgcagcg acattctcaa gcaccacatg gaagtgcgcg ggctgggcat
    20521 cctgagcatc aaagatttgt tcggtattgc gtcggtgcgc gatgccaaaa aagtcgatct
    20581 ggtcatcgag ctgtccgact ggaatcccga cgatgaatac gatcggctgg gaattgacga
    20641 tctgcgcttc gccatcctcg acgtggcgct gccgcttctg aaaatcccgg tccgtcccgg
    20701 tcgcaacata gccgcgatca tcgaagtggc tgcgcgcaat cagctgctca aacagcaagg
    20761 cattcactcg gcacgcgagt ttcaagatcg actgaccgcc gcgatgcagc agacccgccc
    20821 gcacagcggc atggcgaccc ccgaagacat cgaatagctg tccgcagcac agtttgcccg
    20881 aggcctacgt gagttccgaa gagcagagca tgcacatctt ggtggtgacc ggcgtctcgg
    20941 gcggcggcaa gtcaactgcg ctgcgtgcgc tggaggacat cggttactac tgcgtcgaca
    21001 acctgccgct gccgcttctg ccgcagtttg ttcagcttgt cggaaacacc ggacagaaaa
    21061 aagccgcgct ggtgattgat gcccgcgaag gcgagttcct gggtggtttc cgtcacgaaa
    21121 tgcgcgagct gcggtcgaaa ggtctggcgc tagagattct gtttctcgac gcgccggatg
    21181 atgtgctgct gcgccgtttt tccgaaaccc gacggcgaca tcccctgcac ggcgacgatc
    21241 tgcgcgctgc gatcgcgcac gaacgcaaag agcttctgcc gctgcgagaa gaggcgcaca
    21301 ccgtcgtcga taccggcgcg ctcaatgtgc atcagctcaa aggcgtgatc ggcgaacgct
    21361 acggcaaaag cggtcaagtg ctggcactga cgctgctgtc attcggtttc aaatacggac
    21421 tgcccgtcga agcggacatg gtgctcgatg tccgattttt gcccaatccg tactttatcc
    21481 cggcgctgtc gaatcagacc gggcttgaac ccgaggtatc cgactacgtc ttaagtcagc
    21541 ccgacgccca gacatttgtc gccaaggccg aagcgctgat cgaattttat cttccgcgtg
    21601 cgcagcgcga aggcaagtcc tacgtaaccg tggcgatcgg ctgtacaggt ggccgacatc
    21661 gctcggttgc tgtggttgcg gatcttttca atcgactcgg cgcacggtat caaattactg
    21721 ttagacatcg cgagctgggt cgagggagag aaccatgaac gccccgttta ctgctgtggt
    21781 ggtcgtgaca cacggacggg ctggagccga catgctggat gcggctcaag cgtttttgca
    21841 catgcagctc cctctggttg ctgcggtcgg cgtaaacccc gacgatgatc agtcggtggt
    21901 aaacgccaaa attgatcagg ccctgacaga cctgggcatc gacggctggg aagatgtgct
    21961 ctttctggtc gatctcgtcg gcagcacccc agcgcggctc tgctgtggca agtgcggtga
    22021 caaaggtcat gtcgtcacgg gtctcaacat gcccatgctg gtgaagctgg caactgcgga
    22081 tcgcagcacc ggacctgccg cgcttggatc ggcgctggtg ctgaccggaa ccaagtccat
    22141 tcacgtcgag tagccacgat gatgctgtcc gcaaaactca aaattgtgaa caaagccggg
    22201 ctccacgcac gggcgtcgac gcgcttcgtt caggccgcca acaaatatcg cagcgcagtc
    22261 agcgtcaaga aggacggcca agaggtcaac ggcaagagca tcatgggcgt cttgttgctc
    22321 gtcgcgtcga tcggcagcga gatcaccgta tcttgcgatg gcgatgatgc gaaggaatgt
    22381 ctacagactc tcacggagct ggtaaattcc ggattcggtg aaaactaagg atgccgatgg
    22441 gacttggtga cgaaagtggc ccctatccgg cgctgaggga accgattcgc cccctcgccg
    22501 aagaggtgcg cacaggcatt gccgcagcgg ctggaattgc cgtcgggcaa gcattcgtca
    22561 tcgatcgccg aaggcaaccg acgccacgac gccatctgct accggaagaa atcactgccg
    22621 agctggtgcg gctcgatgaa gcggtctctc tcgcggacat gcagcttgaa cggctgaagc
    22681 gcaagctcaa ggagtccgac gagagtgact cgagcgtgat tttggaagcc catcagctga
    22741 ttttgcggga cgaacacctg ctcgggccga ccagacgacg catccgcgac gaaaagctca
    22801 atgtcgagtg ggcgctgcgc aagaccgtcg ccgaaatcca gcagcgcttt gatgcga
//
LOCUS       DAORGN010000066        22472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_908_length_22472_cov_7.275728, whole genome shotgun sequence.
ACCESSION   DAORGN010000066 DAORGN010000000
VERSION     DAORGN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22472
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_908_length_22472_cov_7.275728"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PKL17_09440"
     CDS             complement(<1..814)
                     /locus_tag="PKL17_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNK44994.1"
                     /translation="MTLFTRRRSGTRAHFVGIGGIGMSGIAEVLLALGCRVSGSDRKL
                     SDVTDRLRSLGAEIREGHSENNLELFDGDLDVVVISSAVQTKNPEVVAARARGIPVIR
                     RAEMLAELMRLRYGIAIAGSHGKTTTSSLIATVLHHAGLDPTAIIGGKLPALGSNARL
                     GQSEYLVAEADESDGSFLHLMPTVAVVTNIDPEHLDHYGTFDKLLTAFRDFVDKVPFY
                     GRAVLCQDHPTVREMLPTVTKKVITYGLTLGAQFRAEELLFSGLSSRFTALKH"
     gene            869..2269
                     /locus_tag="PKL17_09445"
     CDS             869..2269
                     /locus_tag="PKL17_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK44995.1"
                     /translation="MKLAVWWMRQAGAASPVWARVGLLAAVVMLFGHGTRARAHDPDH
                     PDVKTAVEWWGPLWSEIAPDTATLRVGFADGKPRPCTLRVDEVSQRDRNAAPLRVVQS
                     ASAAWHLLRVESLSPSRRYRYTLDCPDLSPFAGEFSTAPPIGDPRPLLLAVFGDEQAS
                     PNNDSVSSRSIVQAVLAEAPDLVIGTGDLVGEGGSKDDWRELSKTHQPLWSQLLYLPA
                     LGNHELLGDYDGSFFRRLLPQAESRFYAVRYGFLQLIFLDGNQPGSQPQTHFLATQLK
                     STQGDKSVRARIVVLHQPPLSVGLHCGTSGSMHEWMHIFEKYRVDAVLAGHDHAYERL
                     ERNGVPYFVTGGGGAKLYDQSPCGQPDEPALQRYESVHHYLMVELKPLADRVEISLRA
                     QVPQGMPFDKVTLPLAPSAVGQIHKDSDEPGPPRRWHRGHLLYVLRHHGLQLGVALVF
                     GAVVVAAWRYGRRRSS"
     gene            2282..3433
                     /locus_tag="PKL17_09450"
     CDS             2282..3433
                     /locus_tag="PKL17_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNK44996.1"
                     /translation="MRIGSRLGSLAISDIRAMTIACQAKGGINLGQGVCDMPTPPPVA
                     RGAVRAIDDQLSTYAHPMGIAELRQAVANKIESFYGVAYDPNREVVITNGATGGFAAA
                     VLALCEPGDEIILFEPYYGYHLNTVLSLGLRPVLVPLKPPTWEITREALSRAVTSKTR
                     AIVICTPSNPSGKVLSAAERDLLADFCESHDLIAFCDEIYEHIVFDGKQHIPLQKLPN
                     MRSRCVTLTGLSKTFSITGWRIGYLTTTPEAASRIAIAHDLLYVCAPTPLQYGGLAGL
                     VEMPRSYYDELSAMYQKKRDILCQALADVGLTPFWPTGAYYVLADVRKLGCQTAREAA
                     YKLLDEVGIAAISGSSFYQDPVGETMLRFCFAKPDDVLEEAAARLRRRL"
     gene            3706..5847
                     /locus_tag="PKL17_09455"
     CDS             3706..5847
                     /locus_tag="PKL17_09455"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44997.1"
                     /translation="MNRSKLRWLINPLFLLFTATCGDSGTSGIDPNCLSGVDSDNDGA
                     TDDVECNLGTDPKNADSDGDGVPDGTEITIGSNPQSLDTDGDGVPDGTEVNYPKACIA
                     SDNFAQVRPPLSCTMDSECAPGEKCSGMDPTKPDTDGDGVLDGLEDKNLDGTINATSG
                     ETDPRLWDTDGDGGSDKDTGSKICRPDGLGMVTVDQVSGSSHQIGYDPIFGADKKVVG
                     TTQNTGAVLVDDAGTGVSGMSISKLTTAADVRADATAAEAVVTTELPKIAGTAVVAVL
                     IGRALTTHELLPAITSTYRVTTGAGITASRLRDQLVAPFIGTAAPAGANVGAAAEFYL
                     DITTVRRGGSVDIIIAVSPRTLYDDASKATAIRVNDLVNSTGVATPNKTLDFDCQGFK
                     ADRNPMADFLWTVDVSGSMGPYQTALGNTANAFVNRLKSAGVDMRVGVVAAGEQPINF
                     TTPGFKFISGGSAMAALDLCRQVTSNGLGKCPLDAAETLSPYAMAGGSEEPIAAGVVL
                     HDQLKKNAAMGITNSDYLLRPGATKVVFGVTDEPGSNDFSRYFQTAKDPDTGVAFGTV
                     YNAQTLANIVGYYKRNNVLTFGYVPVDLTRNCTTTLNVADLPRCVIEGNGGAAIPIKT
                     VFNQADIDAGMNRIVDAVAGAASQFKLLRSPITSTIKVNVRNTDVPRSRLDGFDYDPV
                     SKAIVFYGSTYRPQIGDQVYISYRVWAGSTN"
     gene            complement(5844..8516)
                     /locus_tag="PKL17_09460"
     CDS             complement(5844..8516)
                     /locus_tag="PKL17_09460"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNK44998.1"
                     /translation="MRIPSRLLLLSATLAACSVPPSEQSDLPPKMMMEDACPYESAPP
                     RAGVGQTISAGAVLAGTAEVRIDLPVGTPQGGYTARMKALGGQAPDYRRSVHAKAFVP
                     SAGVQTVPLLRAVYLQAGSEPTILVKADLCVAFDRLVFDLEKALTAAGIKDVRGRVIF
                     TSSHTHAGPGTFQGTFHLSLGFDLFQEEQYQRLLQSATQAVLAAQKSAKPAKIGAGIW
                     DGWDRNDEIYADRRSEDDNFPGPDGKPIGKHKEQRLLLLRIDDEANQPLAVVTSFPMH
                     GTIGGSDNPLFSTDATGHVELALEERFSRPVTVIHLQGPAGDAAPRGSFGLGRCDDKT
                     TLCENFARMESVGELAAPRIFDLWQSVKTTGTAALEVVTRSVRNGRDISVRGGMTYAP
                     YQADRDIDGSPAAIFNSDGSARFPVTQFNVPVAAGLCGDKKPSLPVDGIPGATGSPYA
                     SCAELGAAQKFIAAILKVEEPALPTCETTRTTLSALRLSGIPLTLRTQKGSGTPTEDK
                     LDNETLVLATLPGEPVSLLADLLRKKSPAGADRTFVVGYAQGHVGYLLDVENWLHGGY
                     EPSINIFGPLEGEWLVERSLDLLKLVVTPEREDAEAPGLGSPSQGARFDRLQFAPRTP
                     QRIVVSASPLAGQLPTAIPSDLFVRTRANPPVSAQPAAQISRVSGRATLVFHGGDPEE
                     DTPQVSLQKETASGSGNFTDVRNRGGRVVGSRGRDILLFYTPSPVDAEPGKATAHLWT
                     AEWQAVGVSTDGSAPLDAALALPLGRYRLSVSGQSGGKPYRIDSQPFTVTADSAITLS
                     ATRSGNKVSGQAFYPVGSGFRLIRLSGPSDGNVPVLGMLKVTVQSRKDGKQETVSASS
                     SDGSVGFATTVDGSAGFVIDVEDAAGNHGTQTIN"
     gene            complement(8522..10240)
                     /locus_tag="PKL17_09465"
     CDS             complement(8522..10240)
                     /locus_tag="PKL17_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK44999.1"
                     /translation="MDGSSPGEPAAISDTSSACGDTGALSPSGFTVIGGDQVILPSCP
                     KKKLPGEALGIGGRHVLFVNYDGADIRVGDNSMENLGIRDPGFKNQGIIELDGFAPSS
                     ASRAQDIAKIHQQVQKWYADFDIDVVMSRPLSGDYQMTLVGGDKTDIGLGGGIVGISP
                     GDCKNNSETNLNFAFSRSLTERPDQVAVTIAHEAGHAYGLGHVQNQKDIMYPSVSVVD
                     GFLGGQVADAGPCGAKPGDYQDSKKVLLENLGARQGEAPGKSTPPTLKLVSPRDGESV
                     GKDVTLAVQAQAERGIDHITLSLSRIDGGKSRGAHPVAELRPPQSVAQVRLSSAGSYQ
                     LTATAYDRVGNISVSSVSFVAATPTCAVPNDCAPGQKCQNNTCVTPPVPPPSPTGMGD
                     ENLRAMGQACEKSAECKEGICAITTVGQICTHYCTPDRLCAGGLECVDGACLPQMYSK
                     TTPKIGALGGKCSRNQDCSTGECSAPGADPKAPRYCTKVCDPEIGWSCPSTMECVQTD
                     GATGMKNRCVTRGTGAMGTASGCAVSDPGATSEASGVRLFGGSAALVLLGLWAKTRRR
                     RSGPAL"
     gene            complement(10418..11896)
                     /locus_tag="PKL17_09470"
     CDS             complement(10418..11896)
                     /locus_tag="PKL17_09470"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45000.1"
                     /translation="MTNGMVAKKLRVGQMLKVKAQALSPNGQGVVAEHVPAQIHVRNL
                     LPSEEATVVVRHVSSHGGPAHADVSRRHSCAEARVVPSCASFGPCGGCAVLHMSYSAQ
                     LAWKTACVKTELAAVQVSVNDCIAAPFPSDYRCRVKLVATSHPVRKVMLGAFAPRSHQ
                     VLDMAGCETNRRSLMLVAKTVAEEAAKLAIAPYDEESAVGSLRYVLLREVASGQVQVS
                     LVLADRPQQVRALADAIAARHPAVSSITLHRNASRGNALLPAPTPFGERQPETNSGLE
                     KKSLSEEEKELGSTDDDEIIVGDKFLWEELTVPLRVSARSFLQVNREVAKRMYSEAAR
                     QLAHLSFDTVLDLYCGVSGLGLTVLRDHPDARLFGIELGASAIADAEASAAALGFSPP
                     KANFFVGKVEEVLPSLQAMATFGRTAAIINPPRRGCSEEALSALCHSAPSHIIYMSCS
                     PASLARDLQYLIARGYVAKLVMPFDMHPGTQHIECLAVLERK"
     gene            12096..12770
                     /locus_tag="PKL17_09475"
     CDS             12096..12770
                     /locus_tag="PKL17_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45001.1"
                     /translation="MPKKTARTKAAATKKAPARRAPAAAKPKTSAQELQRLINQMVVE
                     RQHHIDSIAEIDQTFKEFGIASGSPARGGKRVKGKAKDADTAAGKAVKTPGKKKVAKK
                     GGKKAAAPKAPKAAKAPKAPKAPKAPKAPKAPKAPKAAKAPKKAKPSRKGVKPGKFEI
                     TGDQLILDFLKSSGGASTEDIRKHWESNGRRGKAENNLTNLVKSGKIKRIKRTDGPGS
                     RYELTA"
     gene            12875..13186
                     /locus_tag="PKL17_09480"
     CDS             12875..13186
                     /locus_tag="PKL17_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45002.1"
                     /translation="MSERLEELLRFVELMPQDPFPLYGLALEYRSVGKFAESAAAFER
                     LLASHPAYVPQYLMYGKLLAGELANVEKAREVLVRGIERARQAGNRHALGELQSELDA
                     L"
     gene            13195..13428
                     /locus_tag="PKL17_09485"
     CDS             13195..13428
                     /locus_tag="PKL17_09485"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNK45003.1"
                     /translation="MSTPTRPVFLELAAGRHLLCRCGQSARLPFCDGSHRDSHHEPLA
                     VDVEVSRRVALCTCKASEKFPLCDGAHKNVRPR"
     gene            13486..14055
                     /locus_tag="PKL17_09490"
     CDS             13486..14055
                     /locus_tag="PKL17_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HNK45004.1"
                     /translation="MNGKSHRPLSFLFATCILFALPACPGPQVKPDSAKNASSAEAVT
                     TFEARSGSAISGDATLRQDGDSVRIVVNVKGATAGEHGLHIHEKGDCSAPDAASAGPH
                     FNPLGHNHGGMEQSPRHAGDLGNITVNADGTGTLEVVLKGLTVASGDLSVVGRALVLH
                     EKPDDLRTQPSGNSGARIGCGVITVKTGN"
     gene            complement(14052..15668)
                     /locus_tag="PKL17_09495"
     CDS             complement(14052..15668)
                     /locus_tag="PKL17_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45005.1"
                     /translation="MSRYQDALARNELDETESLEQEPVEAEVSEGDDGGPANDPVRMY
                     LKRLGDTPLLTREGEVAVGRKMAEGKRQVLLAVLSCHHSIVELLAIKDRLLSGKLRVR
                     DVVSDSEIEQDDESDAETVESLLLQRLVAQLDGLGKRYADLQKKQRALRNASRPPSVK
                     RKRELLLEVSRLRDQLVDDVAAMRFHPRLIDALSQKVKTMAQQAEQLRQSHQKIEDRA
                     GMPASSIALLIADQGKKTEAQRRAQLRKLGLRAEELDELARQIKDIDAKMVQLERETR
                     LEVSELCKLGSDIERGEKLANRARRELLEANLRLVVSIAKKYTNHGMQFLDLIQEGNL
                     GLMRAVDKFEYQRGYKFSTYATWWIRQAITRGIFDQSRTIRIPVHVFETARKLFRITN
                     ELSRQLARDPTPEEISEASHLPLGKVRQVLKVAAREPLSLETPSPVREGSSLGDTIED
                     HATPSPAESLIDAALVRKTRTMLKLLPVREQQILRMRYGIEQDGDNTLEEIGQNFGLS
                     RERIRQIEVQALRKLRHSGSTRHLKSFVEP"
     gene            16226..18739
                     /gene="rnr"
                     /locus_tag="PKL17_09500"
     CDS             16226..18739
                     /gene="rnr"
                     /locus_tag="PKL17_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNK45006.1"
                     /translation="MELVSTDGILQILSSGSHPLGMKATEIVERSGADKKTRHRVRRL
                     LAELLEAGQIERGPGKRYCIAGTVALETPAPLAKAPKPSGDKVSQASAASVSLKPPVE
                     TALPVDSVIGRILVHPGGFGFVQRDDGGDNVFVAARYRGVAMDRDKVALKVWQGFRGP
                     EGRVLTILERGRARLTGTVDLRDYQAMLLPDDPRITNRVFLDGDVPRSIVGKAVVAEI
                     TEYPRSEREPMSARLLAVLGAPDDPRTEIQKILACEEIEETFPKAVDHESLTVPDRVT
                     EEDLVDREDLRHVPFVTIDPETARDFDDAVCVEPGSENGVLRLWVAVADVSYYVRPGT
                     ATDREAERRGVSVYLPDRVVPMLPLPLSAGICSLNPLVERLAMVARLDIDRHGFVKDR
                     KLMAAVIRSRARFDYAGVAQVLFGEPSGKHAHYEPHMEQLKLLGEVATRLRAERVRRG
                     GLDFDLPEAKVILAADDPRLVVTVKRSRADALVRRAYQMIEDAMLAANEAIAEFFAER
                     GLDVLWRVHPPPRREPLTRLAELATSFGVPLDPEAATEPLALRTFLEQIKGKPCERAL
                     SYMVLRSLNQAQYAVENAGHFGLAARRYLHFTSPIRRYPDLVVHRLLKLILRQEGEPA
                     GKVLGAWPPRKEMKEWADDSSLRERKAMVVERQVVDLYRTLLMRERIGEEFDGNVSGV
                     TTAGLFVEIDDPYVEGLVRSERLDEEFELNDKTLRYESDRSGHGIGLGDRVRVRIDSV
                     SVPQRRIELSLVSAAGVLPKTTRKRSLETSRKDEPATEKKRSKRSDRPATAKKSAAGK
                     TAKPGSRDAKSKSAPKKAHSTSGNSSKRTKKKTDRKPRR"
     gene            18783..19139
                     /gene="queF"
                     /locus_tag="PKL17_09505"
     CDS             18783..19139
                     /gene="queF"
                     /locus_tag="PKL17_09505"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNK45007.1"
                     /translation="MASRPSKKLETFANPSKERDYEIRFDCPEFTCLCPLTGQPDFAH
                     FRISYVPDELCIELKSLKLYLWSFRDEGAFHEKVTNQIADDIISAIRPRKLRVEGDFA
                     VRGGIGTVVVIEHQGR"
     gene            19148..20338
                     /gene="mutY"
                     /locus_tag="PKL17_09510"
     CDS             19148..20338
                     /gene="mutY"
                     /locus_tag="PKL17_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNK45008.1"
                     /translation="MAGRRKSPIDTEPTAHDAPAPFAKKSSLSDIRVSLLDWFRQGHR
                     DLPWRKERDPYAIWISEIMLQQTRTETVKGYFARFVARFPTVSSLAAAPLDDVLSLWS
                     GLGYYSRARNLHKAAQQIRDLHHGEFPTEPAAIAALPGIGPYTAGAIRSIALGQAAPI
                     LDGNVIRVLSRLFLLLQRPETAAGKRLYWSLAESLLPAPTGSAPRNDPGDLNQSLMEL
                     GATVCLPRRPICLSCPLSTYCEARRVGNPEDYPPAKAQRAVPTVKNVSLILLCDADVL
                     LLRRPELGLWGGLWEPPTLALDEGETEQAGLLRLGKDRLGITLPVEKARPLAPFTHVL
                     THRIMRFAPYVLSVPSLPMLRLDGYTAQRLLRPDSPQSIGLSAWVSSLLARVVEDSSQ
                     PGLF"
     gene            complement(20360..22189)
                     /gene="argS"
                     /locus_tag="PKL17_09515"
     CDS             complement(20360..22189)
                     /gene="argS"
                     /locus_tag="PKL17_09515"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNK45009.1"
                     /translation="MSSATQVPTSLSSACTQLGKRAAQALADKFANNPAIPTELQLLP
                     ASKPEFGDLQVNACLQLAKPLGQKPRDLAQVVLSSLTDHPAVAKAEIAGPGYVNVFLT
                     DGFLSGCLSTLGNDPRHGIEQLHVGKTVVVDYSSPNIAKPMHIGHIRSTIIGDALRRV
                     FSSVGYRVIADNHLGDWGTQFGKLIVAYRSWIDESAFARDPIGELVRLYQKFVQEEKA
                     QADALQLTRTAHTGDDEEDDDATAQVTPLLAKARAELAKLQQGDAENLKLWKHFVTVS
                     LAEFDKTYARLGVKFDTVYGESHFHPRLASLVEELRQKGIAEDSRGAVICNVDGAPAP
                     LLIRKADGSFLYGTTDLATIEQRVRDYQPERVLYVVGSPQQLHFQQVFFIARKMGVTC
                     SLEHISFGSMRFKDKDGNYTTGSTRKGNVPLLDEFLDLATERAAQVAKQKNDELPDGE
                     LADVARVVGIGAIKYNDLSRDRQQDIHFDIDKALALEGNTAPYMQYAYARLRSIARKA
                     QNEGAATADSVTIVEPAERRLARRLLDYAAAVETVARTARPHHLCEYLFDLAGAVSTF
                     YTEVPVLKAQPEARASRLKLLALVAETLKHGLSLLGIEVPERM"
BASE COUNT         4380 a         6644 c         6827 g         4621 t
ORIGIN      
        1 cgtgctttag cgccgtgaag cgactgctca agccggaaaa caagagctcc tcggcgcgga
       61 actgggcccc gagcgtcaga ccgtaagtga tgaccttttt ggtgacagtg ggcagcatct
      121 cgcgcaccgt cgggtgatcc tggcacagca cggcacgacc gtagaacggc actttgtcga
      181 cgaaatcgcg aaatgccgtg agcagcttgt cgaacgtccc gtagtgatcc aggtgttctg
      241 gatcgatgtt ggtgacgacc gccaccgtcg gcatcaggtg caaaaaggaa ccatcggatt
      301 catcggcttc cgcgaccaaa tattccgact gaccgagccg cgcattgctg cccagcgccg
      361 gcagtttgcc accgatgatc gcagtcggat cgagtcccgc atggtgcagc accgtcgcaa
      421 tcagcgaact cgtggtcgtt ttcccgtgtg agccggcaat cgcgatgccg tagcgcagac
      481 gcataagctc ggcgagcatt tcggcgcgtc gaatcaccgg gatgccgcgc gcccttgccg
      541 cgacgacttc tgggtttttc gtctggactg cgctcgaaat gacgacgaca tcgagatcgc
      601 catcgaacag ctcgagattg ttttcgctgt gtccttcgcg gatttcggcc ccaagggatc
      661 gaagtcgatc ggtgacgtcg ctgagcttgc gatcgctgcc cgacacccga cagcccaagg
      721 cgagcagtac ctcggcaata ccggacatgc ctatgccgcc gattccgacg aagtgggccc
      781 tggtccctga ccgtctgcgg gtaaagagcg tcatggtggg caaagctacc acaaacggcc
      841 accacacaaa ggcgctacag tggcggcgat gaaattggcc gtttggtgga tgaggcaagc
      901 gggcgcggcg tcgcctgtgt gggcgcgtgt cggtcttctg gccgctgtgg tgatgctgtt
      961 tggtcatggc acgcgggcgc gggcgcacga tcccgatcat cccgatgtca agaccgccgt
     1021 cgagtggtgg ggaccgctgt ggtccgaaat cgcgcccgat acggcgacgc tgagggttgg
     1081 atttgccgac ggaaagcctc gaccgtgtac gctgcgcgtc gatgaagtga gtcagcgcga
     1141 ccgcaatgcg gcgccacttc gggtggtaca gagcgcgagc gccgcttggc accttttgcg
     1201 cgtcgagtcg ctttccccca gtcgacgcta tcgctacacg ctggactgtc ccgatctgtc
     1261 gccgtttgcg ggggaatttt cgactgcgcc accgatcggc gacccgcgac cgctgctttt
     1321 ggcggtcttt ggcgatgaac aagcgagtcc aaacaacgac tcggtaagct cgcgatcgat
     1381 tgtccaggcg gtgctggccg aagcgcctga tctggtgatc ggaaccggtg atctcgtcgg
     1441 ggaaggcggc agcaaagacg actggcgcga acttagcaag acgcaccagc ccctgtggtc
     1501 gcaacttttg tatctgccgg cgctgggcaa tcacgagctg ctgggtgact acgacgggag
     1561 tttcttccga cggcttctgc cacaggccga gtcgcgcttt tacgcggtgc gttacggttt
     1621 tttgcagctc attttcctcg atggaaacca gccgggcagc cagccgcaga cgcacttttt
     1681 ggcgacgcag cttaaatcga cgcagggtga caagtcggtg cgggcccgaa tcgtcgtttt
     1741 gcatcagccg ccgctgtccg tcgggcttca ctgcggtacg tcgggcagca tgcacgagtg
     1801 gatgcacatc tttgaaaagt atcgcgtcga cgcggtgctg gccggacacg atcatgccta
     1861 tgagcgactg gaacgaaacg gtgtgccgta ttttgtgacg ggcggcggcg gcgcaaaact
     1921 gtatgaccag tcgccgtgcg gtcagcccga tgagccggcg ctccagcgtt atgagtcggt
     1981 gcatcactac ttgatggttg agctcaaacc gcttgccgat cgcgtcgaaa tttcgctgcg
     2041 cgcgcaggtg ccgcaaggga tgccgtttga caaagtgaca ctgccgcttg cgccgagtgc
     2101 ggtcggacag atccacaaag attcggatga accaggcccg ccccgtcgct ggcatcgggg
     2161 ccatctgctt tatgtgctgc ggcatcacgg gctccagctc ggcgtcgcgc ttgtttttgg
     2221 cgcagtcgtg gtagcggctt ggcgatatgg gcgtcgacga tcctcgtgat acactgcgca
     2281 gatgcggata ggttctcgac ttggttcgct ggcgatttcc gacattcgcg cgatgaccat
     2341 cgcgtgtcag gccaaaggcg gcataaatct cgggcagggt gtctgcgaca tgccgacgcc
     2401 gccgccggtg gcgcgcggtg cggtgcgggc gatagatgat cagctttcga cgtatgcgca
     2461 cccgatgggc atcgctgagc ttcggcaagc ggtggcaaac aagatcgagt cgttttacgg
     2521 cgtcgcctac gatcccaatc gcgaagtggt gattaccaat ggcgcgacgg gcggatttgc
     2581 cgcggcagtg ctggcgctgt gcgaacccgg cgacgagatc attttgttcg agccctacta
     2641 cggctatcac ctcaacacgg tgctgtcgct cgggcttagg ccggtgctgg tgccgctaaa
     2701 accaccgacg tgggagatca cgcgcgaagc gctgtcccgg gcggtgacga gcaagacccg
     2761 cgcgattgtc atctgcacgc catcgaatcc gagcggcaag gtgctgtcag cggcggagcg
     2821 cgatctgctg gccgattttt gcgaaagcca cgacctgatc gctttctgcg acgagattta
     2881 tgaacacatc gtcttcgacg gcaaacagca catcccgctg caaaaactgc cgaacatgcg
     2941 ctcgcgctgc gtgacgctga ctgggctttc gaagacgttt tcgatcacgg gctggcggat
     3001 tggctacctg acgacgacgc cggaagccgc gagccgcatc gcgattgccc acgatctttt
     3061 gtacgtctgt gcgccgacgc cgctgcaata cggtgggctc gccgggcttg tcgaaatgcc
     3121 gcgcagctac tacgacgagc tgtcggcgat gtatcagaaa aagcgcgaca ttttgtgtca
     3181 ggcgcttgcc gacgtgggac tgacgccgtt ttggccaacc ggggcgtatt acgtgctggc
     3241 cgacgtgcgc aagctgggct gccagaccgc gcgcgaggcg gcgtacaagc tgctcgatga
     3301 agtgggcatc gcggcgatct cggggtcgag cttctatcaa gatcccgtcg gtgaaaccat
     3361 gctgcgcttc tgttttgcca agcccgacga cgtgctcgaa gaagccgccg cccgtctgcg
     3421 ccgtcgcctg tagacttccc gcccgccgtt cgcttcccgc atttcaaacc caatcgctgc
     3481 tgccgcttgt cgctggtggg tgctgtcctg cttgcgtgat tggcagatag cgccgtcatt
     3541 ccccacaatg gattcggaat attaattcac aattatacgt aactaaaaaa gcactttgga
     3601 ttgattgtaa aatggagaag catcttgcag tggggtgcgg gatgctggca tctcagacga
     3661 agaacttgcc ttgatccaga ccattaacac ttcaggggaa cactcatgaa tagatcgaaa
     3721 ttgcgttggc taatcaaccc tttattcctt ctttttactg ccacttgcgg tgacagcgga
     3781 acatcgggga tcgatccaaa ctgcctgtcg ggtgttgatt ccgacaacga cggcgccacc
     3841 gacgatgtcg agtgcaacct tggcaccgat ccaaagaatg ccgacagcga tggcgacggc
     3901 gttcccgatg gcaccgagat cacgatcgga tcgaacccgc agtcgctcga taccgatggt
     3961 gacggtgttc ccgatggcac ggaagtcaat tatccaaagg cctgcattgc gtccgacaac
     4021 tttgctcagg tacgtccgcc tctttcgtgc acgatggatt cagagtgtgc gccgggtgaa
     4081 aagtgcagcg gcatggaccc gaccaagccc gacaccgatg gtgacggcgt gcttgatgga
     4141 ctggaagaca agaacctcga cggcaccatc aatgcgacca gcggtgagac cgatcctcgg
     4201 ctgtgggata ccgacggtga cggcggttcg gacaaagaca ccggatcgaa gatttgccgt
     4261 cccgacggac tcggcatggt gacggttgat caggtttcgg gctcctcgca ccagattggc
     4321 tacgatccga ttttcggcgc cgacaaaaaa gtggttggca cgacccagaa cacgggtgcg
     4381 gtgctcgtgg atgatgccgg aaccggcgtt tcgggtatgt cgatctcgaa gctgaccact
     4441 gccgccgatg ttcgtgccga tgcgacggct gccgaagcgg tggttacgac cgagctgccc
     4501 aagatcgccg gtactgccgt cgttgccgtt ctgattggtc gcgcactgac gacgcacgaa
     4561 cttttgccgg ccatcacgtc gacgtatcgg gtgacgactg gtgcgggcat tacggcttcg
     4621 cgtcttcgtg accaactggt ggctccgttc attggcacgg cggctccggc tggtgccaac
     4681 gtcggagcgg cggcggaatt ttatctggat atcaccacgg tgcgtcgtgg cggatcggtc
     4741 gatatcatca ttgccgtctc gccgcgcacg ctgtacgacg atgccagcaa ggccaccgcg
     4801 attcgcgtca acgatctggt gaactcgacg ggagtggcga cgccgaacaa gacgctcgac
     4861 ttcgactgcc agggcttcaa agccgaccgc aatccgatgg ccgacttttt gtggacggtc
     4921 gacgtatcag ggtcgatggg tccgtatcag acggcgcttg gcaacaccgc caatgcgttt
     4981 gtaaaccggc tcaagagcgc gggcgtcgac atgcgtgtcg gcgtcgttgc tgctggtgaa
     5041 cagccgatca actttacgac gccaggcttt aagttcatca gcggcggcag cgcgatggcg
     5101 gcactcgatc tgtgccggca ggtcacttcc aatggtcttg gcaagtgtcc gctcgacgcg
     5161 gcggaaacgc tgagcccgta tgcgatggcc ggcgggtcgg aagaacccat cgctgctggt
     5221 gtcgtgctcc atgatcagct gaagaaaaac gccgccatgg gcatcaccaa ttcggattat
     5281 ctgctgcgtc cgggcgcgac caaggttgtg tttggcgtca ccgatgagcc gggatcaaat
     5341 gactttagcc gctacttcca aacggccaaa gaccccgaca ccggtgtcgc attcggaacg
     5401 gtctacaatg cgcagacgct ggccaacatt gttggctact acaaacgcaa caacgtcctg
     5461 acgttcggat atgtgccggt cgacttgacg cgcaactgca cgacgacgct gaatgtggcc
     5521 gatttgccgc gctgcgtgat cgaaggaaac ggcggcgccg caattcccat caagactgtg
     5581 ttcaatcagg cggacatcga cgcaggcatg aaccgaatcg ttgatgccgt cgcgggcgcg
     5641 gcgagccagt ttaagctgct gcggtcgccg atcacgagca cgatcaaggt aaacgtccgc
     5701 aacaccgacg taccgcgctc gcggctcgat ggctttgact atgacccggt ttcgaaggcg
     5761 attgtgttct atggatcgac gtatcgtccg cagatcgggg atcaggtcta catctcgtat
     5821 cgcgtgtggg ctggctcgac gaactagttg atcgtctggg tgccgtggtt tcccgcggca
     5881 tcctctacgt cgatcacaaa ccccgcgctc ccatccactg ttgtcgcaaa tcccacactg
     5941 ccatcgctgc tgcttgcgct tacggtttcc tgcttgccgt ctttgcggct ctgcacggtg
     6001 actttcagca ttcccaacac cggcacgttt ccgtcgctgg gaccagaaag tcggatcaaa
     6061 cggaaaccgc tgccgaccgg ataaaaggcc tggccgctga ctttgtttcc gctgcgagtg
     6121 gctgacaacg tgatggcgga atcggcggtg acggtgaacg gctggctgtc gatgcggtag
     6181 ggcttgcctc cgctttggcc actcaccgat aggcgatagc gaccaagcgg cagggcgagc
     6241 gctgcatcaa gcggtgcact tccatcggtt gagactccca ccgcttgcca ttccgcggtc
     6301 cacaggtgtg ccgtcgcttt tccgggttcc gcatcgacgg gacttggcgt gtagaaaagc
     6361 aggatgtcgc gacctcggct accgacgacg cggcctccgc gattgcgcac atcggtgaag
     6421 ttgccgctgc cggaagcggt ttctttttgc aggctgactt gtggcgtgtc ttcttccgga
     6481 tcaccaccgt ggaacaccag cgtcgcacga ccgctcacac gcgaaatctg cgccgctggc
     6541 tgtgccgaaa cgggcggatt tgcgcgggtt cggacaaaca gatcggaagg aatcgcagtc
     6601 ggcaattgtc cagcgagcgg tgaagccgac accacaatcc gctgcggtgt gcgcggcgca
     6661 aactgcaatc gatcaaaacg tgcgccctgc gaaggacttc ccagccctgg cgcttcggca
     6721 tcttcgcgct cgggagtgac caccagtttc agcagatcca gcgaccgttc gacaagccac
     6781 tcaccctcga gcggaccaaa gatattgatc gacggttcgt agcccccatg cagccagttt
     6841 tccacatcga gcagatagcc cacatgtcct tgcgcatagc cgaccacaaa cgtgcgatcc
     6901 gctccggctg gcgatttttt gcgcagcaga tccgcgagaa gtgacaccgg ttcaccgggc
     6961 agagtcgcga gcaccagcgt ttcattatcg agcttgtctt ccgtcggtgt gccgcttcct
     7021 ttttgcgttc gcaaggtcag cggaatgccg ctcagccgca gcgcactcag cgtcgtgcgc
     7081 gtggtctcgc aagtcggcag cgcaggttcc tctactttca gaatggcggc gatgaatttt
     7141 tgcgcggcgc caagctcggc gcacgatgcg taaggacttc ccgttgcacc aggaattccg
     7201 tcgacgggaa gcgatggttt tttgtcgcca cacagacccg cggccaccgg cacattgaac
     7261 tgcgtcaccg gaaaacgcgc actgccatcg gagttgaaga tggcggctgg gctgccatca
     7321 atgtcgcgat ccgcttgata cggcgcatag gtcattcctc cgcgcacgct gatgtcacga
     7381 ccgttgcgca ccgatcgcgt gaccacttcg agcgcggcag tgccggtggt tttcaccgac
     7441 tgccacagat cgaaaatgcg cggcgcggcg agctctccga cggattccat cctggcgaag
     7501 ttttcacata gcgtggtctt gtcatcgcag cggcccagtc caaaactacc gcgcggtgcc
     7561 gcgtcgccgg ctggaccttg cagatgaatc acggtcactg ggcgcgaaaa gcgttcttcc
     7621 agtgccagct cgacgtgtcc ggttgcgtcg gtggaaaaca gcggattgtc actgccgccg
     7681 atggtgccgt gcatcgggaa gctcgtcacc accgcgagag gctggttcgc ttcatcatca
     7741 atgcgcaaaa gcaaaagccg ctgttctttg tgcttcccaa tcggttttcc atctggccct
     7801 gggaagttgt catcttcgct gcggcggtcg gcataaattt cgtcgttgcg atcccagccg
     7861 tcccaaattc cggcgccgat ctttgccggc tttgccgatt tctgcgctgc caggaccgct
     7921 tgcgtcgccg actggagcag tcgctggtac tgctcttcct gaaacagatc aaacccgagc
     7981 gacagatgga acgtgccttg aaaagttccc ggtcccgcgt gggtgtggct gctcgtgaag
     8041 atcacgcgac cgcgcacatc ttttattccg gctgcggtca gcgctttttc cagatcgaac
     8101 accaaccgat caaacgcgac acacagatcc gctttgacca agatcgtggg ttcgcttccg
     8161 gcttgcagat acactgcgcg cagaagcggt acggtctgca cgcctgccga cggaacaaac
     8221 gctttggcat ggacgctgcg acgataatcg ggtgcctgtc ctcccagcgc tttcatccga
     8281 gcggtgtagc caccttgcgg tgttccgacc ggaagatcga tgcgaacttc tgcggttccg
     8341 gccaggactg caccagcgga aatcgtctgt ccgaccccgg cccgtggcgg cgcgctttcg
     8401 tatggacaag cgtcctccat catcatcttc ggtgggaggt cggactgttc ggaaggaggg
     8461 acgctacaag ccgcgagcgt cgcagacaga agcaggagac gggagggaat gcgcatggga
     8521 atcacagggc cgggccgcta cgtcgtcgtc gcgttttcgc ccacagaccc aagagcacga
     8581 gcgcagcgct gccgccaaac agacgcacac cgcttgcttc actggttgcg ccgggatcgg
     8641 acaccgcgca gcctgacgct gtacccatgg ctccggttcc gcgcgtcacg cagcgatttt
     8701 tcatcccagt ggcgccatcg gtctgcacac attccatggt ggaaggacac gaccagccga
     8761 tctcgggatc gcagactttg gtgcagtagc gcggcgcttt cggatcagca cccggtgcgc
     8821 tgcattcgcc ggtggaacag tcttggttgc gagaacactt gccaccgagc gcgccaatct
     8881 ttggtgtggt cttgctgtac atctgtggca agcacgcgcc gtcgacgcac tcgagtccgc
     8941 cggcacacag ccgatccggc gtgcagtagt gggtgcagat ttgaccgacg gtcgtgatcg
     9001 cgcagattcc ctctttgcac tctgcgcttt tttcacaagc ctgacccatg gcgcgaaggt
     9061 tttcatcgcc catcccggtc gggctgggtg gtggcacagg tggtgtcacg caggtgttgt
     9121 tttggcactt ctgtcccggc gcgcagtcat tgggaaccgc acacgttggc gtcgccgcga
     9181 caaagctgac cgacgatacc gatatgttgc cgacccggtc atatgcagtc gccgtcagtt
     9241 gatacgatcc ggcgctcgac agacgcactt gcgcgacaga ctgcggcgga cgaagttcgg
     9301 caaccggatg ggcgccgcga gatttgccac catcaatccg gctcaagctc agcgtgatgt
     9361 gatcgatgcc gcgttcggcc tgggcctgca ccgccagcgt gacgtctttg ccgaccgatt
     9421 ccccatcgcg tggactgacc agcttcagcg tcggtggagt cgattttcca ggcgcttctc
     9481 cctgccgtgc cccgaggttt tccagcagca cttttttgct gtcctggtaa tcgcccggct
     9541 tggctccgca cggtcctgcg tccgcgactt ggccgcccag gaatccgtcg accaccgaca
     9601 cgctcgggta cataatgtcc ttttgatttt ggacgtgtcc caaaccgtag gcgtggccgg
     9661 cctcgtgcgc gatggtcact gcgacttggt cgggtcgttc cgtaagcgag cgcgaaaacg
     9721 cgaagtttag gttggtctcg ctgttgtttt tgcagtcgcc gggggaaatg ccgacgatcc
     9781 cgccgcccag accgatgtcc gtcttgtcgc cgccgaccag cgtcatttgg tagtcgccgc
     9841 tgagcggtcg gctcatcacg acatcaatat cgaagtcggc gtaccacttt tgcacctgct
     9901 ggtggatctt ggcgatgtct tgcgcgcggc tggcgctgct cggtgcaaaa ccgtcgagct
     9961 cgatgatgcc ttggtttttg aaaccaggat cgcggatgcc gaggttttcc atcgagttgt
    10021 caccgacgcg gatgtcggcg ccatcgtagt tcacaaacag gacgtgccga ccgccaatgc
    10081 cgagcgcttc accgggcagc ttttttttcg gacagctcgg caggatgact tggtcgccac
    10141 ctatgaccgt gaaaccggaa ggtgacaacg cgccggtatc gccgcatgcg gaagaagtat
    10201 cgctgatcgc cgctggctca ccggggctgc ttccatccat gcctgcctgt gagtcgcaac
    10261 cgagcgccac cgtggcgcac gaaatgagag ccaaagttcc ccattttgtg tggccaaaag
    10321 cgcggttttc tcgtcgagaa gtggcagaaa aagcgaaggt gtgcggcttc cgttgcatgc
    10381 gtgcaacgta catggacctt ttcggaagtg gaaggggcta ctttcgctcg agaaccgcaa
    10441 ggcactcgat gtgctgggtg cccggatgca tgtcaaacgg catgacgagc tttgccacgt
    10501 agccgcgcgc gatcagatac tgaagatccc gcgccaagct ggctggactg cagctcatat
    10561 agatgatgtg ggaaggcgcg ctgtggcaca gggcagaaag cgcttcttcg ctgcagccgc
    10621 gccgcggtgg attgatgatc gccgcggtgc gaccaaacgt cgccatggct tgcagggatg
    10681 gcaggacttc ttcgactttt ccgacgaaaa agttcgcttt tggtggggaa aaacccaagg
    10741 cagccgcgct cgcttctgca tcggcaatcg cacttgcgcc cagttcgatg ccaaaaagcc
    10801 gcgcatccgg atgatcgcgc agcaccgtga gtccaagacc tgacacccca caatacagat
    10861 ccagcacggt atcaaaggaa aggtgggcaa gctggcgcgc ggcttcgctg tacatccgct
    10921 ttgcgacttc acggttgacc tgcaaaaacg aacgcgcgga cacccgcaag ggcaccgtaa
    10981 gttcttccca caggaactta tcaccgacga taatttcatc atcatcggtg cttccgagct
    11041 ctttttcttc ttcgctcagg ctcttttttt caagtccgga attggtttcc ggttggcgtt
    11101 caccaaaagg tgtgggcgca ggaaggagag cgtttccgcg gcttgcgttg cggtgcagcg
    11161 tgatggatga caccgcggga tgacgcgctg cgatggcatc tgcgagcgcg cggacttgct
    11221 gcggacgatc cgccaaaacc aggctcactt gcacctggcc gctggccact tcacggagca
    11281 gcacgtagcg caagctaccg acggcagatt cctcatcgta gggcgcaatc gccagctttg
    11341 ccgcttcttc cgcgaccgtt tttgccacca gcatgagcga ccgccgattg gtctcgcacc
    11401 ctgccatgtc cagcacttgg tgggagcgtg gcgcaaacgc gcccagcatg accttgcgca
    11461 ctgggtggga agttgccacc agcttgacgc gacagcgata atccgacggg aaaggagccg
    11521 cgatgcagtc attcacagag acttggaccg cggccagctc cgttttcaca caagcggtct
    11581 tccatgccag ctgcgcggag tacgacatgt gcaagaccgc acaaccaccg cacggaccaa
    11641 agcttgcgca gcttgggaca acgcgcgctt cggcacagga atgccgtcga gaaacatctg
    11701 catgcgcagg tccaccatgg ctagacacat gccgtacaac caccgttgct tcttcgctgg
    11761 gcagcaggtt gcgcacatgg atttgcgcgg gaacgtgttc cgcgaccact ccttgaccat
    11821 ttggcgaaag tgcctgtgcc ttcaccttga gcatctgccc aactcggagc ttttttgcca
    11881 ccattccgtt tgtcatcacc gtctccaatt gctgccattc cttcccttct cccaccctgt
    11941 acacggagcc agtccggacc acaagagaag gcggtcagga atcttcccaa agcattccgt
    12001 ccgcaaaaaa aggaaagagt ctgcttgtac cagaaacgcg agttgtgtaa gtttcatctc
    12061 agcgtgtatt aacgaaagga attggggtaa caaagatgcc aaagaagact gcccgtacca
    12121 aagctgccgc gaccaagaag gcccctgcgc gtcgtgctcc tgctgccgcg aagccgaaga
    12181 cctcggctca ggagttgcag cggctcatca accagatggt tgtggagcgg cagcatcaca
    12241 tcgattcgat tgcggagatc gaccagacct tcaaggagtt cggcatcgcc tcgggtagtc
    12301 cggcgcgcgg tggcaaacgg gtcaagggca aggccaagga tgcggacact gccgcgggca
    12361 aggctgtgaa gaccccaggc aagaagaaag tcgccaaaaa gggtggcaaa aaggctgcgg
    12421 ccccgaaggc tcccaaagct gcgaaggctc ccaaagcccc gaaggctccc aaggccccga
    12481 aggctcccaa ggccccgaag gctcccaaag ctgcgaaggc ccccaagaag gccaagccga
    12541 gccgcaaggg agtgaagccg ggcaagttcg agatcacggg tgatcagctc atcctcgact
    12601 tccttaagtc ttccggcggc gcctcgaccg aggacatccg caagcactgg gagtcgaacg
    12661 gtcgtcgtgg caaggctgaa aacaacctga ccaacctggt gaagtcgggc aagatcaagc
    12721 gcatcaagcg caccgacggt ccgggcagcc gctacgagct gactgcgtag ttctattcgg
    12781 gctctgattc cttccggagc ccatcgtttt ttcttccgtc ccctctttgc tgcggactcc
    12841 gagttcgcgc cacttcactt acaggatgaa tgccatgtcg gaacggctcg aagagctttt
    12901 gcgcttcgtg gaactgatgc cgcaggaccc atttccgctc tatggactcg cgctagagta
    12961 tcgcagcgtg gggaagtttg cggagtctgc ggcggctttt gaacgactcc ttgcatcgca
    13021 tccggcgtat gttccgcagt acctcatgta tgggaaactg ctggcgggcg agcttgcgaa
    13081 tgtcgaaaaa gcgcgcgaag tgctggtgcg cggaatcgaa agagcaagac aagcgggaaa
    13141 ccgccacgca ctcggtgagc ttcagtccga actagacgcg ctatgatact tgctatgtct
    13201 acgcctaccc gaccggtctt tttggagctt gctgcgggaa gacatctgct gtgccggtgt
    13261 ggacagtctg cgcgattgcc gttctgtgat ggctcgcacc gcgattccca tcatgaaccg
    13321 ctggccgtcg atgtcgaagt ctcgcgtcgg gttgcactgt gtacttgtaa ggcgagtgag
    13381 aagtttccac tttgcgacgg tgcacataag aatgtgcgac cccgctgagc gctcgttttc
    13441 ccaaaaacca aaaccttccc caacatcctg caaggagttt tcgtgatgaa cggcaaaagc
    13501 catcgcccgc tttcgtttct gtttgccacc tgcattcttt tcgcactgcc cgcatgtccg
    13561 ggcccgcagg tgaaaccgga ttcggcaaaa aacgcgagct cggctgaagc ggtgacgacg
    13621 ttcgaagcgc gcagtgggag tgctatcagc ggcgatgcga cgcttcgaca agatggcgac
    13681 agcgttcgca tcgtggtgaa cgtaaagggc gctaccgctg gcgagcacgg tctgcacatc
    13741 catgaaaaag gcgactgctc tgctccggat gccgcatcag caggcccgca tttcaatcca
    13801 cttggtcaca atcacggtgg tatggagcag agcccgcgcc atgcagggga tctggggaac
    13861 atcacggtga acgctgatgg aaccggaacg ctggaagtgg tgctaaaagg tctgactgtg
    13921 gcaagcggag acctttcggt ggtgggacgt gcgctggtgc ttcacgaaaa gcccgatgat
    13981 ctgcgcacac agccgtccgg aaattccgga gcccgcattg gctgcggcgt gatcacggtc
    14041 aaaacgggaa attagggttc cacaaacgac ttcaggtggc gggtgctgcc cgagtgacgg
    14101 agcttgcgca gcgcttgcac ttcgatttga cgaatccgct cgcgggacag accgaagttc
    14161 tgcccaatct cttcgagcgt attgtcccca tcttgctcaa tcccatagcg catccgcagg
    14221 atctgctgct cacgcacggg cagaagtttt agcatcgtgc gcgtcttccg cacgagcgct
    14281 gcgtcaatca gcgactccgc gggtgacggc gtcgcgtgat cttctatcgt atcacccaag
    14341 ctcgatcctt cacgaaccgg actcggtgtt tccaaagaca gcggttcccg cgcagcaacc
    14401 ttcaacacct gacgcacttt gccgagaggc agatgactcg cttccgatat ctcctccggc
    14461 gttggatcgc gggcaagctg tcgcgaaagt tcgttggtga tgcggaacag cttgcgcgct
    14521 gtttcaaaca catggaccgg gatgcgaatg gtgcgcgact gatcaaaaat gcctcgggtg
    14581 atcgcctgac gaatccacca ggtggcatag gttgaaaact tgtagccgcg ctggtattcg
    14641 aacttgtcga cggcgcgcat cagtcccagg ttgccttcct gaatcagatc aagaaactgc
    14701 atcccgtggt tggtgtactt tttcgcgatc gacaccacca gacgaagatt cgcttcgagc
    14761 agctcgcggc gtgcgcgatt cgcgagtttt tcaccgcgtt cgatgtcgct gccgagcttg
    14821 cacagctcac tcacttcgag gcgcgtttcg cgttcaagct gcaccatctt cgcgtcgatg
    14881 tctttgatct gcctggccag ctcatccagt tcttcggcgc gaagtcccag tttgcggagc
    14941 tgtgcgcgtc gctgtgcttc ggtcttctta ccttggtccg caatcaaaag cgcgatcgac
    15001 gatgcgggca ttcctgcgcg atcctcgatc ttttgatgac tttgccgaag ctgctcggcc
    15061 tgctgcgcca tcgtcttcac tttttgtgac agcgcatcaa tcagtcgtgg gtgaaaccgc
    15121 atcgcggcga catcatcgac cagctgatcg cgcaggcgag acacttccaa aagcagctcg
    15181 cgcttgcgtt tcacagacgg tggtcgactg gcattgcgga gcgcgcgctg cttcttttgc
    15241 agatccgcat agcgcttccc aagtccgtcg agctgggcca ccagccgctg caaaaggagc
    15301 gattcgaccg tctctgcatc cgattcgtca tcctgttcga tttccgaatc agagacgaca
    15361 tcacggacgc gcagcttgcc gcttagcagt cggtctttga tcgcgagaag ctccacgatc
    15421 gagtgatggc aagacaggac agccagcaaa acctggcgtt ttccttccgc cattttgcga
    15481 cccacggcga cctctccctc tcgtgtcaaa agaggcgtgt cgcccagtcg tttcagatac
    15541 atccgaaccg ggtcgttggc gggaccaccg tcgtcgcctt cgctgacttc agcctcgacc
    15601 ggctcttgtt cgagcgactc ggtttcgtcc agttcgttac gtgccagtgc atcttgataa
    15661 cgactcatag gctcgtctcc ggggcgatgg ccccatgcat aaggtcaaac ttctgctcga
    15721 ttgacactgt gtctctcggc agctttctca cctcgccgtc acgatctagt tggaccgaaa
    15781 accatcgccg acatttcatt gcgtgggttt ttttgtgtgt cccacgagtc ttgtgcgatc
    15841 ggtccgtccg ctcacttccg tgaagtgtct ctactgcttt gatgccaagc ggaaagctgc
    15901 ttttgccgct tctaacaagc gtagaccgcg aaagcttcca gacaagtcga agtgaaaaat
    15961 cttttttcgt tgtcttgtcg cggcactttg gggaagttgc cttgcaaaaa gcgaaaagcg
    16021 atctaccatt tgtgcgtttt gactccacgg gcgtgctcct tctggctcgc tcaccacaca
    16081 aacgcagaaa ctcaccttcc gatccgtgtc tcaaaaatca gacgtagtgg ccgaggactc
    16141 tgttccgaac gtgcccgtac catccccaga ctgtccccca tcaaccatcc aaccaaaccc
    16201 ttctgacgaa gccgacattt cgcgcgtgga actcgtcagt acagacggca ttttgcaaat
    16261 cctttcgagc ggcagtcatc cgctgggaat gaaggcgacc gaaatcgtcg agcgaagcgg
    16321 tgccgataaa aagacgcgcc atcgtgtccg ccgattgctt gcggaactgc tggaagccgg
    16381 acaaatcgaa cgcggccccg gcaagcgcta ctgcatcgct ggaactgtcg cgctggaaac
    16441 gcctgcacct ttggccaaag cgcccaaacc gagcggcgac aaggtttcgc aggcatccgc
    16501 ggcttccgtt tctttgaaac cgccggttga aacggcgctt cccgtcgatt cggtgatcgg
    16561 acggattttg gtacatccgg gcggcttcgg ttttgtgcag cgcgacgacg gcggcgacaa
    16621 cgtctttgtt gcggcgcgct atcgcggcgt tgccatggac cgggacaaag tcgcactcaa
    16681 ggtgtggcag ggattccgcg gtccggaagg cagagtgctc acgatcctcg aacgggggcg
    16741 tgccaggctg accgggacgg tggatcttcg cgattaccag gcgatgctgc tgcccgatga
    16801 tccgcgcatc acaaaccggg tcttcctaga cggtgatgta ccgcgcagca tcgtcggaaa
    16861 ggccgtggtt gcagagatca cggaatatcc gcgcagcgag cgagagccga tgtcggcgcg
    16921 actgcttgcc gtgcttggtg ctcccgatga tccgcggacg gaaatccaaa agatcctcgc
    16981 gtgtgaagag atagaggaaa cgtttcccaa agcggtcgat cacgagtcgc tgacggttcc
    17041 tgatcgagtg acggaagagg acctcgtcga tcgagaagac ttgcgccacg ttccgtttgt
    17101 gaccatcgat ccggaaaccg cgcgcgactt cgatgacgcg gtgtgcgtcg agccaggatc
    17161 tgaaaacggc gtgctgaggc tctgggttgc ggtggcggac gtgtcgtact atgtccgacc
    17221 gggaaccgcg acggatcgcg aggccgagcg ccgtggtgta tcggtgtatc tgcccgaccg
    17281 ggtggtgccg atgctgccgc tgccgctgtc ggcgggaatc tgttcactga acccgctggt
    17341 cgagcggctg gcgatggtgg cgcgactcga tatcgatcgc cacggctttg tcaaagatcg
    17401 caagctgatg gcggcggtga ttcgatcgcg tgcgcgtttt gactacgccg gcgtcgcgca
    17461 ggtgctgttt ggcgagccga gcggaaagca cgcacattac gagccgcata tggagcagct
    17521 caagctgctg ggcgaggtcg caacgcggct gcgagccgag cgggtgcgtc gcggcgggct
    17581 cgattttgat ctgcccgaag ccaaagtcat tttggccgcc gatgatccgc ggctcgtagt
    17641 gacggtgaag cggtcgcgcg ccgatgcgct ggtgcgacgt gcgtatcaaa tgatcgaaga
    17701 tgcgatgctg gcggcaaacg aagcaattgc ggagtttttt gccgagcgcg gtctggacgt
    17761 tctgtggcgc gtgcatccgc cgccgcgacg ggaacctctg acgcgcttgg ccgaactggc
    17821 gacgagcttt ggcgtaccgc tcgatccgga ggccgcgacc gagccgctgg cgctgcggac
    17881 tttcttggaa caaatcaaag ggaagccgtg tgagcgcgcg ctgtcctaca tggtgttgcg
    17941 gtcgctcaac caagcccagt atgccgtcga aaatgccgga cactttgggc tggcggcgcg
    18001 gcgctatctg cactttacgt ccccgattcg gcgctatccc gacttggttg tgcatcggct
    18061 gctgaagctg attcttcgtc aggaaggcga gccggcgggc aaggtgctgg gggcgtggcc
    18121 gccgcgcaaa gagatgaaag agtgggccga tgattcgtcg ctgcgcgaac gcaaagcgat
    18181 ggtagtcgag cgccaagtcg tcgacttgta tcgaacgctg ctcatgcggg agcggatcgg
    18241 cgaggagttt gacggcaacg tctcgggtgt gacgacggcg gggctgtttg tcgaaatcga
    18301 tgatccgtat gtcgaaggcc tcgtgcgcag tgagcggctc gacgaagagt ttgagctaaa
    18361 cgacaagaca ctgcgctacg agtcagaccg cagcggtcat ggcatcggac tgggcgaccg
    18421 ggtgcgggtg cggattgatt cggtgtcggt gccgcagcgg cggatcgagc tgtcgcttgt
    18481 ttctgctgcg ggcgttttgc cgaaaacgac gcgcaagcga tccctggaaa catcgcgaaa
    18541 agacgaacct gcgacggaaa agaagcgcag caagcgaagc gatcgaccgg ccacggccaa
    18601 aaaaagtgcg gcgggaaaga cggcgaaacc tggaagcagg gacgcaaaat cgaagtccgc
    18661 accgaagaaa gcgcactcga cgtcgggaaa tagttccaag cgcacgaaga aaaaaacgga
    18721 tcgcaaaccc cgccgctgat cgctctttac agaaacgccg ctgccgacta tgaaggccga
    18781 ccatggcgtc ccgcccctcc aaaaagcttg agaccttcgc gaatcccagc aaggagcgcg
    18841 actacgaaat ccgcttcgac tgtccggaat ttacctgcct gtgccccctg accgggcagc
    18901 cggactttgc acactttcgc atcagctacg ttcccgacga gctttgcatc gagctgaagt
    18961 cgctcaagct gtatctgtgg tcgttccgcg acgaaggcgc attccacgaa aaagtcacca
    19021 accagattgc cgacgacatc atttctgcga ttcgcccacg caagctgcgc gtcgaaggtg
    19081 actttgcggt gcgcggcggc atcggcaccg tcgtcgtcat cgagcatcaa ggcaggtagg
    19141 cgaatccgtg gcgggccggc gcaagtcacc gattgatacc gaaccgaccg cccacgacgc
    19201 tcccgcaccc tttgcaaaga agagctcgct ttcggacatt cgtgtgtcgc tgctcgactg
    19261 gtttcgacag ggacatcgcg acttgccgtg gcgcaaagag cgcgatcctt acgcgatctg
    19321 gatttcggag atcatgctcc agcagacgcg aaccgagacg gtaaagggtt attttgcccg
    19381 ctttgtggca cgttttccga cggtgagttc actcgccgca gcgccgctcg atgatgtctt
    19441 gtcgctgtgg agcggacttg gctattactc gcgggcgcgg aacctgcaca aagcggcgca
    19501 gcaaattcgc gatctgcacc atggcgaatt tccgacggag cctgcggcca tcgcggcgct
    19561 gccggggatt gggccgtaca ccgcaggggc gattcgatcg attgcgctcg ggcaggcggc
    19621 accgattctc gacggcaatg tcatccgagt gctaagccgc ttgtttttgc ttttgcagcg
    19681 accggaaacg gcggcgggca agcggctgta ttggtcactt gctgaatcgc tgctgccggc
    19741 cccgacgggg agcgcgccac gcaatgatcc cggcgactta aaccagtcgc tgatggagct
    19801 gggcgcgacg gtgtgtttgc cgcgccgacc gatctgccta agctgtcctt tgtcgacgta
    19861 ctgcgaagcg cgtcgtgtcg gcaatccaga agactatccc ccggccaaag cgcagcgggc
    19921 tgtgccgacg gtgaaaaatg tctcgctgat ccttctgtgc gacgcagacg tgttgctttt
    19981 gcgtcgaccc gagctgggtc tttggggcgg actgtgggaa ccgccaacgc tggcactcga
    20041 cgaaggtgag acggaacaag ctgggctttt gcggcttggc aaagatcggc tgggaatcac
    20101 gcttcccgtc gaaaaggcca gaccgcttgc gccgtttacg catgtgctga cccaccgcat
    20161 catgcgcttt gcgccgtatg tgctctctgt gccgtcgctg ccgatgctgc ggctcgacgg
    20221 ttacactgca cagcggcttt tgcgtcccga ttcgccgcag tccatcggtc tgtcggcgtg
    20281 ggtatcatcg ctgctggcgc gtgtcgtcga ggattccagc cagccagggc tgttttagcg
    20341 tcgctggcga gcttgttcgc tacatccgct cgggcacttc gatgccgagc agcgacagtc
    20401 cgtgtttgag cgtttcagcg acgagcgcca acagcttgag ccgtgaggcc cgcgcctcgg
    20461 gttgggcttt gaggaccggc acttcggtat aaaacgtgct gaccgcacca gcgagatcga
    20521 acagatactc gcacaggtga tgtggacgcg cggtgcgggc caccgtctcg accgccgccg
    20581 catagtcgag cagtcgacgg gccaatcgcc gctctgccgg ttcgacgatc gtcaccgagt
    20641 ccgccgtcgc tgctccttcg ttctgcgcct tgcgcgcgat cgatcgcaga cgggcgtaag
    20701 cgtactgcat atacggcgcg gtgtttcctt ccagcgccag cgctttgtcg atgtcaaagt
    20761 gaatgtcctg ctgacggtcg cgcgacagat cattgtattt gatcgcgccg attccgacga
    20821 cgcgcgcgac atctgccagc tcgccgtctg ggagctcgtc gtttttctgc ttggccacct
    20881 gcgctgcgcg ttccgtcgcc aaatcaagga actcatcgag cagcggcacg ttgcctttgc
    20941 gcgtcgatcc ggtggtgtag ttgccgtctt tgtccttaaa tcgcatcgag ccgaagctga
    21001 tgtgttcaag cgaacaagtc acacccatct tgcgggcgat gaaaaacacc tgctgaaagt
    21061 gcagctgctg cggcgagccg acgacgtaca acacccgctc gggctgatag tcgcggacgc
    21121 gctgctcgat ggtcgccaag tccgtggtgc catacaggaa cgatccgtcg gctttgcgaa
    21181 tcaaaagcgg agccggcgca ccatcgacat tgcaaatcac ggcgccgcgg ctgtcttcag
    21241 cgatgccttt ttgccgcagt tcctcgacga gagatgcgag ccgcgggtga aagtgacttt
    21301 cgccgtacac ggtatcgaac ttcaccccga gccgcgcgta ggttttgtca aactctgcga
    21361 gcgacaccgt cacaaagtgc ttccacagtt tcaggttttc ggcgtcacct tgctgaagct
    21421 tggcaagctc tgcccgtgct tttgcaagca gtggtgtcac ttgcgcggtg gcgtcgtcgt
    21481 cttcctcgtc gtcgccagtg tgtgcggtcc gcgtaagctg tagtgcatcg gcctgcgctt
    21541 tttcttcctg gacgaacttt tggtaaagcc gcaccagctc gccgatggga tcgcgcgcaa
    21601 acgccgattc gtcgatccac gaccgatacg cgacgatcag cttgccaaac tgggttcccc
    21661 aatcgccgag atggttgtcc gcaatcacgc gatatccgac ggaagaaaac acgcgcctga
    21721 gcgcatcgcc aatgatcgtc gaccgaatgt ggccgatgtg cattggcttg gcgatgttgg
    21781 gcgacgagta atcgacgacc acggttttgc caacgtgaag ctgctcgatg ccgtggcgtg
    21841 gatcgtttcc aagcgtcgag aggcagcccg aaagaaatcc gtcggtgaga aagacgttca
    21901 cgtaacccgg accggcgatt tccgctttgg cgaccgcggg atggtctgtc agcgacgaca
    21961 aaacgacttg tgccagatca cgcggtttct gtccgagcgg cttcgcgagc tgcaaacagg
    22021 cattcacttg caggtcgcca aactctggtt tggaggccgg caaaagctga agctccgtcg
    22081 gaatcgccgg attgtttgcg aacttgtcag caagcgcttg cgcagcgcgc ttgccaagct
    22141 gggtgcaggc ggaagacagg gatgtcggca cttgggtcgc ggaagacatg gccgcgaaca
    22201 ctagcaaatc tggttgtctc tcggaagaag aacagcaaag atggaaagcc tcgtcagctc
    22261 ggcgcccacc gagaaggaga ggagcctcgt accggggcgg cggtaacgag ggggcgccga
    22321 gcgaacgagc tgtgagttac ttgcagcagg cgaactttgc gtgacggaac atcgacacgg
    22381 cgccgcacgg atcgtaggcc gcaaaaccat cgagcttcat gccgcgcgct gagcaggtct
    22441 tgtcggcttc cgccttgagg tttgcttccg tc
//
LOCUS       DAORGN010000067        22270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_925_length_22270_cov_6.742156, whole genome shotgun sequence.
ACCESSION   DAORGN010000067 DAORGN010000000
VERSION     DAORGN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22270
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_925_length_22270_cov_6.742156"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKL17_09520"
     CDS             complement(<1..573)
                     /locus_tag="PKL17_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45010.1"
                     /translation="MPKQILLVEDSVTMQKVVQIAFAKEDYQIVAVSSADEALARLKE
                     SRPDVLIIDAGLTGKNGYDLTASARAEANGKDVPVLLLTSNFNPYDEARGQRASVNAN
                     LVKPFDTQSVIDKVNALVKGGPVAAAAAPAPTPAAKPAPQPQVQAPPPAAAPVQQLGT
                     AALRPPSPVTPPAAKPKESAAIATVAAPTTP"
     gene            complement(833..2110)
                     /locus_tag="PKL17_09525"
     CDS             complement(833..2110)
                     /locus_tag="PKL17_09525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK45011.1"
                     /translation="MTPRPTLRVTACPNLSEVAKSDWNALLEPDDAPLLSWEYLQGLE
                     QTRCVGEGTDWLPLHILIHRHPDETSEDRQLVAAAPAYIKLSSHGEWVWDEEWPDLSA
                     AFGVSYYPKLVLAVPFNPVTGGRLLTLPTLSAAERRDLRGLLLRSAQLVCSSENLSSA
                     HVLFVRGPAFRSEQTPDLGDQLDVVSQQHFWPRRQTQYHFHNRGYRSFDDFLADLRSH
                     RRNTIRRERRELSEAGITVQTHAGLRNDETAPPVTDGKNRGFTWAELDQMFAMYLGTS
                     VRYTGEAPFLNQAFFRLCSERLGDQLELVLARNRDGNLIAGAWNLRGTTRRFGRYWGE
                     SVPIRFLHFEVCFYHPIERCIADGISAFEPGHGGDQKLLRGFRPTYTYSAHFFSHSNL
                     HQVIGKYLQKESFLVDAVRGFEQQRCPLRTDDE"
     gene            complement(2147..2953)
                     /locus_tag="PKL17_09530"
     CDS             complement(2147..2953)
                     /locus_tag="PKL17_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45012.1"
                     /translation="MISKRTVFLWLLGGAVSVLAAPWANADAPSETVDVSALVGKLTL
                     LSDGKGHYVAVTPMPADQRELFYGDGKTFFQVPLVGASRNGDKWEHAFAEPRLTEGNR
                     GEGSGYVGFDGQSYRVECGKRVTPLTPVAAAESKTILGSGKFVVSPRKFRPYALSRDD
                     RGTYYYVDRGYHDKDRARFRVFIGQKGNLKPQQMTNVVNDPSGDIFSTKTGTLRLVLN
                     KDPQELKWVFGKKSSPLVRVSLDINTMIASIYNEFGVYSGERLGTPCDDL"
     gene            complement(2943..3752)
                     /locus_tag="PKL17_09535"
     CDS             complement(2943..3752)
                     /locus_tag="PKL17_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45013.1"
                     /translation="MMSRFVSTLCALCGLVALSQTTTAKAEAPQEIPAADRAKTTVCT
                     DGKLHYVVMAPDERLISALFYGNDKQLYRVPAESSGMLSGGHFLEPRFIAATKNPSFR
                     GVDMRIYSEVVVDKEKSTCEVYCGERKVALQVVPADKATQLLSTAKFVENPRQFVPYA
                     LARDDRGTYYYVDRGATPITASRFRLFRGPRGNLKLQQMVNVVNDSEGDVFSTKSGSL
                     RMILDKRESTWVENQKPSKLTLVPVQQNLNVIYNDLGVYAGQRQGTPCDDL"
     gene            3829..5436
                     /locus_tag="PKL17_09540"
     CDS             3829..5436
                     /locus_tag="PKL17_09540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45014.1"
                     /translation="MPADSQSATLPEPGLAGSSRPDAMVGRVLKEKYRVEAQLGQGAM
                     GVVYRGTQLGLGKTVAIKMIRADILVTKDSHDRFSREAQVLSKLLHPGIAQVLDFGIE
                     DGTPFLVMEFVDGKELTEVMQLEGPMAPIRAIAIVRQLAAALEEAHRNGVVHRDIKPH
                     NIRLQRYSPTGPIYLKVLDFGIAKQLGDENGTSLTATGAVIGTPAYMAPEQAGGTKID
                     ARADQYAVGIVLYELLTGTVPFVSDTVTGVLVSHLTKPPPPLPREVPEPLQRIVMRLL
                     EKEPSGRYADISSVEKALADCEGYCRDARPLAKGTIRPTVAGTALVGGNKRSTGTILG
                     AAVLAAVVVSGVILGSAKLRGRHDAAGGDGAKASSGQGSAGVAAATVNPAGPGPAAAA
                     SNPPVGPSVTTNAGADKGTTTAADRPAETKPADKPVTDKPTTTGNTTGSKPHAAGAGS
                     GLEPPAVKEQLDRAEKLLQDGDYPKAIELARQTTFTHPTPRAFRQLTYAHCKMGNFVA
                     KATFFKVAPADRKKLIAVCQENGVLLR"
     gene            complement(5459..5842)
                     /locus_tag="PKL17_09545"
     CDS             complement(5459..5842)
                     /locus_tag="PKL17_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45015.1"
                     /translation="MTKPINQLLITAFAPILIVTGVLGFVTPPSLSLMSGAAPYNLFH
                     IFFGIVGVGLALTRRLPLARAFHIGFGAIDLYQAVASFAGIFPTALFAYRRADDVLHV
                     LLGLLLVGVGVLADRTAIAEKPKAP"
     gene            complement(5839..6117)
                     /locus_tag="PKL17_09550"
     CDS             complement(5839..6117)
                     /locus_tag="PKL17_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45016.1"
                     /translation="MRSLWHTDNMVRSGAALGAALLLLAFLVYATIALGSTDVGSVLH
                     DVSPGPGDGQNSQPWLIIGASAVAILVGLAVTLAASFLPKSTPSRPPQ"
     gene            complement(6216..8291)
                     /locus_tag="PKL17_09555"
     CDS             complement(6216..8291)
                     /locus_tag="PKL17_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45017.1"
                     /translation="MVSKLGAGAMATVYLAEHPRINKKVAIKVINQDLATSKEMVSRF
                     MTEARAASQIGHDHIVDILDFGQSPEGENFMIMEYLEGQTISSRIRAAGRLDVMAALH
                     IAMQIVDALQTAHAKGVIHRDLKPDNIYLIRRAGTGDYVKILDFGLAKLLSGTEGQNH
                     KTSSGSVLGTPHYMAPEQCEGKTTIDGRADLYSVGCIMYQMLTGELPFPGEGFAEVLI
                     KHLSEPPPVVRSLNPQIPASVEKLILHCLAKSRDHRFQTAEELLWALHDPESWSLQFG
                     DDMMRIVGPLPGRGVAMLPPTMPTMHAGGVPSALTAAGQPPISPVSTVNIAPGSAPAL
                     PSRPSVPIAAVTVAAGSSPALPPVLGPGQSAARPGIPPAVMPPSALDIPVVAADAAQL
                     AEVPADASRAAPQMATLISDLSSASLSALLRSPQGMLPGQTPAQAPASMPTLAPPAPG
                     PLREMQQSDAQAPPEELSTLDGNSVAGLSAQLRPQGAQSQPGIVPGAASGLHPAALPP
                     PSPPLQTAPASPLQKLAALPPVQRFLALPEQKRTMILGIAGGVVGVLLLILAVVIALP
                     SSVSIEVRSNPPDVEVVRDGKLIGKTPLTLKLGKGEKARLLLRKEGFEEVERVIAGSN
                     QAPIDIELPAEPTERPTPQPEKPPVDNPKTQADASKPEPGKNDPGKAQVKTPPKGTKK
                     GPKKKPAIF"
     gene            8606..10000
                     /locus_tag="PKL17_09560"
     CDS             8606..10000
                     /locus_tag="PKL17_09560"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNK45018.1"
                     /translation="MEKIVALTKRRGFIFQSSELYGGINGAWDYGPMGVLLKRNVKDA
                     WWRAMVHDREDIVGLDSAILMHPMVWKSSGHVDGFSDPMIDCKTCKKRFRADDMDGAI
                     CGQKPSKSPQNCGIADLTEPRNFNLMFETFLGPVSDSSAKTFLRPETAQGIFANFANV
                     ISTTRVKVPFGIAQVGKSFRNEINPRNFTFRSREFEQMEMEFFCVPGTDDDWFKKWVE
                     IRKRWYTDLGLSDKKLRLRDHEKLAHYSKATTDIEYLFPFGWGELEGIANRTDFDLRQ
                     HQRGVRTVGKWDGGDLAAVELAAEDAEYQKGKLSYFDDEKKQRYVPYVIEPAAGADRA
                     TLAFLCDAYAEDSAPDAKGQVETRVVLRLHPKLAPIKAAILPLMRKDGHPERARKIYD
                     DLKKRFFVDYDESAAIGKRYRRQDEIGTPICITVDYDTIKDGTVTVRDRDSMEQIRVS
                     EDKLRGLLEEKLGM"
     gene            10062..10436
                     /locus_tag="PKL17_09565"
     CDS             10062..10436
                     /locus_tag="PKL17_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK45019.1"
                     /translation="MKRALFGCLWAVMALGCGGTSDGTACPAIAASNFTVTVTDAVTS
                     ARICDATVSATDTSTGSSMNLMVFGGATDCAYSGGFYERPGTFTLTAQKSGYLPTTMP
                     NVVVTKGVCNVTPAQVSLKLGK"
     gene            complement(10440..11276)
                     /locus_tag="PKL17_09570"
     CDS             complement(10440..11276)
                     /locus_tag="PKL17_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNK45020.1"
                     /translation="MSDRQVIAAIYAHPDDGEFFAAGTLAKWAQAGHTVYAICATNGD
                     IGSKRRDVSQAQLAEQRAKELGLALSTFGGQPPIMLGFPDGHLAEHAAALRERLIYFL
                     RKLAVDKVVTFDPWKRYEIHPDHITAGKMASEAAVFSCFPLLHPEHLVDGVTARQPQE
                     VWYMMPTEHKPNRLVDISTTFDTKVRSLLCHSSQIEMLADWFVKGADPTQLTNAQRAE
                     LTAGARNFLQMMAQGVAMLKKEVPLAEAFFVQPVGPGHFQNYQQMFQETLGVPPEDPS
                     VE"
     gene            complement(11327..12340)
                     /locus_tag="PKL17_09575"
     CDS             complement(11327..12340)
                     /locus_tag="PKL17_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNK45021.1"
                     /translation="MGERKTAVTVVSGFLGSGKTTLLRRLLSDPNLPQKLAVLVNDLG
                     ELGLDHEIIASETETPTLRVTELVSGCICCTLRSELGEALSALARGDGLARPPEHILI
                     EPSGIARASEVSFAVNALSFDVPVECDAVVTVIDLHNAERAHREHPDLFEDQVRAADL
                     LILNKIDLVPDRDTQTRLSTWLRSLSPRAQQVFADHAMVDARLLLGQIDLSAHLPPDL
                     AKPTTPPIHQGHHPKAHGIAAVTISVPYAIDWNALEDFLDGLSDRIFRIKGIVDRQND
                     TGSATPTTPLLVQAVGDKIDWEELPSDSALSRAPRRLIFIGDPQALDVSQLGDGLLQT
                     RRS"
     gene            complement(12359..13426)
                     /locus_tag="PKL17_09580"
     CDS             complement(12359..13426)
                     /locus_tag="PKL17_09580"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNK45022.1"
                     /translation="MGKPPITANQVTLLRIVLLPLGPYLLYGNPTEKWLALLFMAILG
                     CTDFIDGWLARRYGSTELGRLMDPIADKAFVLVSFLPFIHLMKDDHPWLYPWQVGLLL
                     SREYVITSLRSMYEQRRISPKTPLLAKIKAWAQMAGSGVIFLLRMVPEHWILYAIILG
                     SVLPVVALLIRYAVRRVVWKGALIFGGWFFVLLPIQQLWGSEATAQFLTVTIIGITWI
                     SGFTYITPLFPLLRKGELRAGDWMRLFGSIVPPTLLLILQTQHVLRPWVVMLIMAIEL
                     SVSGLDNLLCFHRVQASAALWGARVGLITLLCALVLAGQPASLLYVALAVSVGLSAIE
                     FYRGRRYYLEEPENAKQRVVD"
     gene            complement(13450..16386)
                     /locus_tag="PKL17_09585"
     CDS             complement(13450..16386)
                     /locus_tag="PKL17_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45023.1"
                     /translation="MQRSHPLTAPRRAACHRVGTLPKLLLAGLVLSGSMFPSGVHRAW
                     AVGEQTARLRGTVVEGGTDVPMQGALVTIRGDSLIGGPRKTTADEEGRFDFPLLPPGK
                     YTVTVEYEGLKALERRVSLELGQTQNLKIPLSAELAKAETQTIIEEKKRLDSDRVSTG
                     KVLTAESQAKLATPRRYQDVVQQLPGVTGSSNPVMAGATQRHNRYLVDGLDITDPVDG
                     TFSANFNFDAIGQMDLLLLAVDAQYNSLGGVINLMTKSGSDKLSIDSSFYVNHQSLSL
                     GGRAGNQLYEGRLLNQSDPSPPVASYQANLNIGGPIVKQKLWFYFSTEYRYRINSVIP
                     GAPLNSQHPPLERHDLYTRLKLTWAPSSRHRLNLSVNADPAWLTNIRNIDGSQTNAYT
                     AEADYFQNQGGVFGILTWDYFATDNLLFGVQTGLQWFGFTTGPQLGDLVSPTHIDNAS
                     TITWNNADAGAIVSDGRLRFQFDPTMTWIQRGGLGQHTIKAGAQFQFLRNYALTGTPG
                     NQIYTDDTSQGGGVLGRDPTSLDRPLGCVEGQPQPKLGSTATPCFQRSLYEPMRAQVR
                     VGWALGAFLQDTWKPTSWLTLIPGIRVDYGTAENSQGQVTQNLLGFGPRLGASVDLTR
                     DGKTMVKFAYGRANEVASLRSAALADVNPMSTTWGYNGATKRFDRFFSSDGGGGGYEL
                     SGRCADGQIRPECGNAKLSLRPPHSDYVTASFERELYANVIGSVTYTYRKLEDLWEDI
                     EINAYRTLDGGNIAGFSDPTRGNVRVYRPTGEAFRRYQAMDIAIAGSPSPAWSVFVAY
                     TLSFLDGTLDDQISVLRNDPPRDLRLYGYLLDDHRHQVKANASYTFHGLTAGVNLAYV
                     TGAPTTRLFATTAFGLQGRYGFRGIDPGADPNDIRKWTELRSSDVLDLSVRAQYDTYE
                     LLRQHLTLIVDIFNVLDLSSATRTNTTSASTYQPAFEARNSAAYGAVTSRQVPFRVQF
                     GLRYQY"
     gene            16545..17585
                     /locus_tag="PKL17_09590"
     CDS             16545..17585
                     /locus_tag="PKL17_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNK45024.1"
                     /translation="MTTPIHIPHRHLGRCGLKVSALTLGAMTFGESETFMRGVTSSDD
                     EARRVLDAALDAGIDTVDTANVYSEGRSEQLLGEWLHGRRDKVTLLTKCRFPIGFGTR
                     PIGPHDQGLSRKHIVQACEDSLRRLRTDYIDLYQVHMQDRNVPIEETLRALDDLVRDG
                     KVRYVGCSNFTGYRLTESLWAADKRDTVRFEAMQIQWSLIVRDAERELVPAAKHFGMG
                     ILVWSPLGRGFLSGKYQKGEAPPEGARLAAWKESYGAMNTEQNWAILDVVRDVAREVG
                     STPAAVSLAYLLAHPECSSIIIGARNLSQLQDNLAAMSVTLSPDQVRRLEQVSRPRFG
                     YPYDFIGLREPW"
     gene            complement(17517..18179)
                     /locus_tag="PKL17_09595"
     CDS             complement(17517..18179)
                     /locus_tag="PKL17_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45025.1"
                     /translation="MTSDPPQRGQQQIDLGGLRWLGLLPLSFFAIHGSRHVLSGHAYE
                     LLWLCTVSCFVVGVGILARSPRWTAAAALWLPLGTVLWIFDACRTGDFLLTSALTHLG
                     GLLVALVWLGRTGFPAGSFVYATGLGVLVQTVSRYVSPHEANVNVSQFIYPGSEAYFP
                     SYRLYWCFIWLSATAIFWICERIGRRIWPGSSASLGDGLPGLAQADKVVGIAKARTRD
                     LL"
     gene            18183..18848
                     /locus_tag="PKL17_09600"
     CDS             18183..18848
                     /locus_tag="PKL17_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45026.1"
                     /translation="MFIRSIWLWLFALSLFAAVPASADPGFVPLPVSAGAPRALEIQV
                     QRYEGSTNGQLTVSIRNPTDRAVKFEAGGLYFVPEQNANDAPQRLGAAGPFAVQKQSG
                     GFQPSDGYTLSPNSQVEVKLDVYCIDSHRRSPRTGDVFRVAVTRMPADLTRSIQSDTS
                     TAARQNGGYAAPAAKSAVQSEIWKNRNKKWIELDGEGVQEKRKDAPAPQMQHIQKRYI
                     DHN"
     gene            complement(18845..19660)
                     /locus_tag="PKL17_09605"
     CDS             complement(18845..19660)
                     /locus_tag="PKL17_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNK45027.1"
                     /translation="MRKVLGSLGIGLLTVGWLSGCGSELSAGADHAGIDGVADAENTD
                     FHAASARVGEKAEVTASSLNLRSGPGTSYAVIASMPKGTIVDVLSESSGFYRVTWGSY
                     TGYCSGDYLVPYSAAGGVADAVSRAESGVGFSYWWGGGCWKPGSSDKGSCTGSCPGCT
                     HGGSYGADCSGYVAKVWQVPGTSSVSTCSHPYSTYNFANETREWRTVARGSAKQGDAF
                     VYNENGAGHIFMMDNGDPWGWMKAYEAKGCSYGIVHGSRTAGTSYKVIRRNGY"
     gene            19905..21497
                     /locus_tag="PKL17_09610"
     CDS             19905..21497
                     /locus_tag="PKL17_09610"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNK45028.1"
                     /translation="MSSRRLNSLWGLLLLVTLGGTQAWGGDRHGRLSAPVHRPTLSPG
                     QGLRPVSGSRETFRLKKPQPLGATRRPLVARPPVPSVAKPMAKPPLGGHLPVVPKPLP
                     VLPHAPVLGKQPPPGPPEYEPPPAGEVAVRWAIPKRRDLGVKIHTAPSYSANVIGELR
                     GQSAMLVGQQIAAKVGLCRTWLSALPSGFVCESDVIVEPGYLSSPPAQEKSTGWQRLR
                     YGVVSSFMTKLRGGGSHYLRQVLHKGDGVTITQEKGEEVQIIGQKWLPRRDVQVFDPP
                     VAPPVALDTVPPGLRLGWAVPPMDEEGVPVFLPKSDSGPVAYLPRYAVVYVVENSDST
                     PGRLQVVFPESTRAVLRAPGSSRPAWVSSPQFEIEVGQLRRMTTAAPPSDLLPDERWI
                     DIHLLEQVATAYQGKKPLFASLVSTGLHGATPPGSFFIYRKYLTQTMANLAGAASQYD
                     FREVPHAQFFNGRIGLHAVLWHDRLGHPVSHGCVNLSPPAAEQFFAFTSPDLPVGWHS
                     VTTFAHQGGPRSAHGTRVVVRK"
     gene            21553..22059
                     /locus_tag="PKL17_09615"
     CDS             21553..22059
                     /locus_tag="PKL17_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HNK45029.1"
                     /translation="MNKAALEILRRGKTLAYSAMTSETGNFLPPVRYAKKAAGFVNDL
                     LGRPLASSAELDRRDEESQRLDALAEKARRGEGAVAKKEAAPVVLYVTDQFVRERKRI
                     EDVLKGRGIPYRVLDLTDDESTRSWALTKAQATEFPLLFVAGESVGGFEQVLSLDATG
                     GLTKKVFG"
     gene            22071..>22270
                     /locus_tag="PKL17_09620"
     CDS             22071..>22270
                     /locus_tag="PKL17_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45030.1"
                     /translation="MELRTLHLDGQTLRTRVYATHPDRVVDVELSSDATARLLTAAAP
                     FLNWLATAGYLPQGASLEALWL"
BASE COUNT         4352 a         6860 c         6802 g         4256 t
ORIGIN      
        1 cggagtcgtc ggtgctgcca cggtggcaat ggcagccgac tctttgggct tcgcagcggg
       61 cggcgtcacc ggcgatggtg gccgcaaagc ggcggtgcca agctgctgaa ccggcgcggc
      121 ggctggtggc ggcgcttgga cctgcggctg cggcgctggt ttggccgccg gggtcggggc
      181 tggtgcagca gctgccgcca cgggtccgcc ttttacaagc gcattgacct tgtcgatgac
      241 cgactgggta tcaaacggct tgacgaggtt tgcgttcacg gaagcacgct ggccccgcgc
      301 ctcgtcatag ggattgaagt tgctggtgag cagcaggacc ggcacatctt tgccgttggc
      361 ttctgctcgg gcagacgcgg tcaggtcata accgttcttt cccgtcaggc cagcgtcgat
      421 gattagtacg tccggtcgcg actccttgag ccgggccagc gcttcatcag cgctcgacac
      481 cgcgacgatt tggtagtcct ccttggcgaa ggcgatctgg actaccttct gcatggtgac
      541 gctatcttcg acgagcagga tttgcttggg catctgtcgt tctttctccc tcggtgtgtc
      601 agctccctag ctggccgatg cacacaggct gtcgcggacg gtggatctca aatggttggc
      661 tgacacagga gtgcagcacg ctgtcgcaat tgtaagtagt cagccatgtt ttgagaacca
      721 tgtaatttcg gtttgcgtgc tctgcgctcg gagctttggg cgggtcgtcg ggatgcaggg
      781 cttggaaatc gggaggcttg cattttttcc gcgccgcggt gacttgccgc gctcattcgt
      841 cgtccgtgcg caggggacag cgctgctgtt caaaaccacg caccgcatcg acgagaaacg
      901 actctttttg cagatacttc ccgatgactt ggtgaagatt cgagtgcgaa aaaaagtgcg
      961 cactgtaggt gtacgtcgga cggaatccgc gcagcagctt ctgatcgcca ccgtggccgg
     1021 gttcaaaagc cgaaattccg tcggcaatgc agcgctcgat cgggtgataa aagcagactt
     1081 caaaatgcaa aaaacggatg ggtaccgatt ctccccagta gcggccaaaa cgccgggtgg
     1141 tgccgcgcaa gttccaggcc ccagcgatca ggttgccgtc gcggtttcgt gccaagacca
     1201 gctctagctg atccccgagc cgctcgctgc acagtcgaaa gaatgcctgg ttcaaaaacg
     1261 gcgcttcgcc ggtgtagcgg accgaagtgc ccaggtacat cgcgaacatc tgatcgagtt
     1321 cggcccaggt aaaaccgcga tttttcccgt cggtaacagg tggagcggtt tcatcgttgc
     1381 ggagcccggc gtgcgtttgt accgtgatgc ccgcttcgct gagctcgcgg cgctcgcggc
     1441 ggatggtgtt gcgacgatgg ctgcgcagat cggccaagaa gtcgtcgaac gagcggtagc
     1501 cgcggttgtg gaagtgatac tgggtttgcc gtcggggcca aaaatgctgc tgtgaaacca
     1561 cgtcgagctg atcgcctaga tcgggcgttt gctcactgcg aaacgcggga ccacggacaa
     1621 acaagacgtg ggctgacgac aggttttccg aactacagac aagctgcgcc gatcgcaaaa
     1681 gcaggccgcg tagatcccgc cgttcggctg ctgacagcgt cggcaaggtg agcagtcgac
     1741 cccccgtcac cggattgaag ggcaccgcga gcaccagctt tgggtagtag ctcaccccaa
     1801 atgccgccga cagatcgggc cattcttcgt cccaaaccca ctcgccatgg ctgcttagtt
     1861 tgatgtaggc cggtgccgcc gcgacaagct gccgatcttc gctggtttcg tcgggatggc
     1921 gatggatcaa gatgtgcagc ggcagccagt cggtgccttc tccgacgcag cgcgtctgtt
     1981 ccagaccttg cagatattcc cacgacagaa ggggcgcatc gtcgggttca agcagcgcgt
     2041 tccaatcgga ctttgcgact tctgacaaat tcggacaggc ggtcacgcga agcgtaggac
     2101 ggggcgtcat gttcgataag gcagcccagc gaaggcgaag aaacgatcag agatcgtcac
     2161 acggcgtgcc caggcgttcg ccgctgtaga ccccaaattc gttgtagatg ctggcaatca
     2221 tggtgttgat gtcgagtgac acccgcacaa gtggcgacga ctttttgcca aagacccact
     2281 tgagctcttg cggatctttg tttagcacga gccgcagtgt tccggtcttg gtcgaaaaga
     2341 tgtcgcccga cggatcgttt acgacgttcg tcatctgctg gggcttgaga ttgccttttt
     2401 ggccgatgaa gacgcggaag cgggcgcgat ctttgtcgtg gtagccgcga tcgacgtagt
     2461 aatacgtgcc gcgatcgtcg cgtgacagtg cgtagggacg gaacttgcgc ggacttacga
     2521 cgaacttgcc gctgcccaag atggtcttcg attcggcagc agcgacgggt gtaagcggcg
     2581 tgacgcgctt gccacattcg acgcggtagc tctgaccgtc gaagcccacg tagccgctgc
     2641 cttcaccgcg gttgccttcg gtgagccgtg gctcggcaaa cgcatgttcc catttgtcgc
     2701 catttctact ggccccgacg agcggtactt ggaaaaacgt cttgccgtcg ccgtaaaaca
     2761 gctcgcgctg gtctgccggc atcggggtga cggcgacata gtggcctttt ccgtcgctaa
     2821 gcaaggtcag tttgcccaca agtgcgctta cgtcgacggt ttcactcggc gcatcagcgt
     2881 tcgcccaagg tgctgccaaa acggacacgg cgccgcccaa aagccacagg aaaacggttc
     2941 gtttagagat catcgcaggg cgttccttgt cgctggccgg catagacgcc gagatcgttg
     3001 tagatcacgt tcaagttctg ctggaccggc acgagcgtca gcttgctggg cttttggttt
     3061 tcaacccacg tcgactcacg cttgtcgagg atcatccgca ggctgccgga cttcgtcgaa
     3121 aacacgtcgc cttcgctgtc gtttacgacg ttcaccatct gctggagctt gaggttgccg
     3181 cgcggaccgc gaaacaaacg gaagcggctg gcggtgatcg gggtggcgcc acgatcgacg
     3241 tagtaatagg tgccacgatc gtcgcgcgcg agggcataag gaacaaactg gcgcggattt
     3301 tcgacaaatt ttgccgtcga cagaagctgc gttgctttgt cagcgggcac gacttggagc
     3361 gcgactttgc gttcgccgca gtaaacctcg caggtggatt tttctttgtc gacgacgact
     3421 tcggaataaa tccgcatgtc gacgccgcga aagctcgggt ttttcgtcgc agcaatgaag
     3481 cgcggttcca aaaaatgacc gccgctgagc atgccggacg attcggcggg cacccgataa
     3541 agctgtttgt cattgccata aaacagcgcg cttatcagcc gttcgtcggg agccatcacc
     3601 acgtagtgca acttcccgtc ggtacaaacg gtggttttgg cgcgatcggc ggcggggatt
     3661 tcctgcggcg cctccgcctt ggctgtggtg gtttgggaga gggcgacgag cccacagagg
     3721 gcacacagag tcgatacaaa tctgctcatc atcgtcgaaa tggtagccga tcgcgcgaag
     3781 ctggtgagtt gttgagcatt ttctcgacgt aaagtaccgt aagctgacat gccagcagat
     3841 tcgcagagtg cgacgctccc cgagccaggg ctcgcaggca gttcgcggcc cgacgccatg
     3901 gtcggtcgcg tgctcaaaga aaaataccga gtcgaggccc agcttgggca aggtgcgatg
     3961 ggggtggtgt accgcggcac ccagcttggg ctcgggaaaa cggtggcgat caagatgatt
     4021 cgcgccgaca tcctggtgac caaagactcg cacgatcgct tttcgcggga agcgcaggtg
     4081 ctcagcaagc tgctccatcc ggggatcgcg caggtgctcg actttggcat cgaagacggc
     4141 acgccgtttt tggtgatgga atttgtcgac ggaaaagagc tgaccgaggt gatgcagctc
     4201 gaaggcccaa tggcaccgat ccgtgcgatc gcgatcgtgc ggcagcttgc tgcggcgctc
     4261 gaggaagccc accgaaacgg cgtcgtacac cgtgacataa agccccacaa cattcggtta
     4321 cagcgctatt caccgacggg gccgatttat ctgaaggtgc tcgattttgg catcgccaag
     4381 cagctcggag atgaaaacgg aacctcgctg acggcgacgg gggcggtaat tggtacgcca
     4441 gcttacatgg cgccagaaca ggcgggcggg accaagatcg atgcgcgtgc cgatcagtac
     4501 gcggtcggaa ttgttctgta cgaactgctg acggggacgg ttccgtttgt cagcgacacc
     4561 gtgacgggcg tgctggtgag ccatctgacc aagccaccgc cgccgctgcc tcgcgaagtt
     4621 cccgaaccgc tccagcgcat tgtgatgcga ctgctcgaaa aagagccgtc gggtcgttat
     4681 gccgatattt cgtcggtgga aaaagcgctg gcggactgtg aagggtactg tcgagacgcg
     4741 cggcctcttg ccaaaggcac cattcgtccg actgtcgctg gtacagcgct tgtgggtggc
     4801 aacaagcgat cgaccgggac gatccttggc gcagcggtgc tggcagccgt ggtggtaagt
     4861 ggcgtcattt tgggatcggc caaactgcgc ggacgacacg atgcggcggg cggcgatggt
     4921 gccaaagcat cgtctgggca aggctcagca ggtgtggcgg cagcgacggt gaatccagcg
     4981 ggtcctggcc ctgccgctgc ggccagcaat ccacctgtcg gaccttcggt gacgacgaat
     5041 gccggcgcag acaaggggac gacgacggct gcggatcggc ctgcggaaac caaacctgcc
     5101 gacaaaccgg tgacggacaa accgacgaca accggcaata cgacggggtc caaaccgcac
     5161 gcggcgggcg ctggatcggg cttggagcct ccggcggtca aagaacagct cgatcgagcg
     5221 gaaaagctgc tgcaggacgg cgactatccc aaagcgattg agctggcgcg tcagaccacg
     5281 ttcacgcacc cgacgccgcg cgcgtttcgg cagctgacct atgcacactg caagatgggc
     5341 aacttcgtcg ccaaggctac gtttttcaag gtcgcgcccg ccgatcgcaa aaagctgatc
     5401 gcggtgtgcc aggaaaacgg tgtgctgctg cgctgatttg gatggcgggc gacgggaatt
     5461 agggggcttt gggcttttcg gcgatggccg ttcgatcggc cagcacgccg acgcccacaa
     5521 gcagtaggcc cagcaggacg tgcagcacgt catcggcgcg ccgataggcg aacagcgccg
     5581 tcggaaaaat cccggcaaag ctggcgacgg cttggtacag gtcgatggcg ccaaagccaa
     5641 tgtgaaacgc tcgcgcaagc ggcagtcgcc gggtcagcgc aagcccaact ccgacgatgc
     5701 caaagaaaat gtgaaacaga ttgtaggggg ccgcgccgct catcagcgaa aggctcggcg
     5761 gggtgacaaa acccagcacg cccgtgacga tcagaatcgg cgcaaacgcg gtgatcagca
     5821 gctgattgat gggcttggtc attgcggggg ccgggacggc gtgcttttcg ggagaaagct
     5881 cgcggcgaga gtgacagcga ggccgaccaa gatggcgacg gcactggcac cgatgataag
     5941 ccacggctgg ctgttctgtc cgtcgccagg tccgggtgag acgtcatgca gcacagagcc
     6001 cacgtccgtc gagccaagcg caatcgttgc ataaaccaag aatgcaagta gaagcagggc
     6061 agcaccgagt gccgcgcctg agcgaaccat gttgtccgtg tgccagaggg aacgcatgtc
     6121 gatagattcc aaaccgccaa actttagcat gtgagtttgt aatttttgcc accaatttat
     6181 gtgacaaaaa agagtcgaag gtcgaggctg cagcgttaga aaatcgcggg ctttttcttg
     6241 gggccttttt tggtgccctt gggtggggtt ttgacctgcg ctttgccggg gtcgtttttg
     6301 cccggttccg gcttgcttgc gtccgcttgc gtcttggggt tgtcgaccgg cggtttttcc
     6361 ggttgcggcg tcggacgttc ggtcggttcc gcgggaagct cgatgtcgat cggcgcttgg
     6421 tttgacccgg cgatgacgcg ctcgacttcc tcgaagcctt ctttgcgcag caaaagacgc
     6481 gctttttcgc ccttgccgag cttgagcgta agcggcgtct tgccgatgag cttgccgtcg
     6541 cgcaccactt ccacgtcggg tggatttgat cgcacctcga tgctgacaga gctggggagc
     6601 gctatcacaa ccgccaagat cagcagcagc acaccgacga cgccaccggc aattccgagg
     6661 atcatcgtgc gtttctgttc cggcagcgca agaaagcgtt gcacgggtgg cagcgccgca
     6721 agcttctgaa gcggactcgc aggggcggtt tgcagcggcg gactgggcgg tggcagcgct
     6781 gccggatgca ggccgctcgc tgcgccgggc acaatccctg gctgggactg ggcaccttgg
     6841 ggccgcagct gcgccgacag accggccacc gagttgccat ccagcgtgga tagctcttcg
     6901 ggcggcgcct gcgcgtcact ttgctgcatc tcgcgcagcg gtccgggggc tggcggcgcc
     6961 aacgtgggca ttgacgcggg ggcttgcgcg ggtgtttggc ccggcagcat cccctgcggc
     7021 gaacggagca gcgccgacag cgatgcggac gacagatcgc tgatcaacgt cgccatctgt
     7081 ggcgcggcgc ggctggcatc ggctggcact tccgcaagct gcgccgcgtc ggctgccacc
     7141 actggaatat cgagcgccga cggaggcatc actgccggcg ggatgcctgg acgagccgcc
     7201 gactggcccg gaccgagcac cggtgggagc gcgggcgatg atccggcggc aacggtcact
     7261 gctgcgatcg gcacgcttgg acgcgacggc aaggcgggcg cggaaccggg cgcgatgttg
     7321 accgtggaaa ccggcgagat cggtggctgg ccggctgccg tcagcgcgct cggcacaccg
     7381 cccgcatgca tggtcggcat cgtcggtggc agcatcgcca cgccacgtcc cggcagcgga
     7441 cccacaatcc gcatcatgtc atcgccaaac tgcagcgacc acgactctgg atcgtgcagc
     7501 gcccacaaaa gctcttccgc ggtttgaaag cggtgatcgc ggctcttggc caggcagtgc
     7561 aggatcagtt tttcgaccga tgccggaatc tgcggattga gcgagcgcac gaccggcggc
     7621 ggttcggaca gatgcttgat cagcacttcc gcgaagccct cgccgggaaa cggcagctcg
     7681 ccggtcagca tttggtacat gatgcagccg acgctgtaga ggtcggcgcg tccgtcgatg
     7741 gtggtcttgc cttcgcactg ctcgggcgcc atgtagtgtg gcgtgcccag caccgatccc
     7801 gacgacgttt tgtggttttg gccttcggtg ccgctgagca gcttggccag cccaaagtcg
     7861 aggatcttga cgtagtcccc cgtgcccgcc cgtcggataa ggtagatgtt gtccggcttc
     7921 agatcgcgat ggatcacgcc cttggcgtgg gccgtctgaa gcgcgtcgac gatctgcatc
     7981 gcgatgtgca gcgccgccat gacatcgagc cggcccgcag cgcgaatccg tgacgagatg
     8041 gtctgcccct cgagatactc catgatcatg aagttctcgc cttcgggact ttgaccaaag
     8101 tcgaggatgt cgacgatgtg gtcgtggccg atctggctgg ccgcccgcgc ctcggtcata
     8161 aaccggctga ccatttcctt gcttgtggcg aggtcctggt tgatgacctt gatcgcgacc
     8221 tttttgttaa tacgcgggtg ctccgccaaa tagacggtgg ccatggctcc cgcgccgagc
     8281 ttggagacca ctctatagtt gcctacagag gagccgatag agatcacagc gtaaatcttc
     8341 ttacgcagcg gccaatttcg caaggcctag catgttaggt ccgctaccta tttttatttg
     8401 tgcgacatgg acttggctgc gtgtgtgggc gcaacggtaa gtgggcagcg cagactggga
     8461 atgtgggtct ttaccgggcc cgcgttggga agcgggatgc aacattgcga cgctcgctcg
     8521 gctgtgtaaa atcgcgccac ctttaggaga cacacccgtg actgcatccg ccccttccac
     8581 ctcggctgac tctggttcct cgctgatgga aaaaatcgtc gcgctcacca agcggcgcgg
     8641 cttcatcttc cagtcctccg agctgtacgg cggcataaat ggcgcttggg actacggtcc
     8701 gatgggcgtg ctgctcaagc gcaacgtcaa ggacgcctgg tggcgcgcga tggtccacga
     8761 tcgggaagac atcgtcggac tggattcagc gatcctgatg cacccgatgg tgtggaaatc
     8821 gtccggccac gtcgacggtt tttccgaccc gatgatcgac tgcaagacct gcaaaaagcg
     8881 ctttcgtgct gacgacatgg acggcgcgat ctgtgggcag aagccgtcga agtcgccgca
     8941 aaactgtggc atcgccgacc tgaccgagcc gcgcaacttc aacctgatgt ttgaaacgtt
     9001 cctgggtcca gtcagcgatt cgtcggccaa gacgtttttg cggcccgaga ccgcgcaggg
     9061 catttttgcc aactttgcca atgtgatctc gacgacgcgc gtcaaggttc cgtttggtat
     9121 cgcccaggtc ggcaagtcgt tccgcaacga aatcaacccg cgcaacttca cgttccgctc
     9181 gcgtgagttc gagcagatgg aaatggagtt cttttgcgtc cccggcactg acgacgactg
     9241 gttcaaaaag tgggtcgaaa tccgcaagcg ctggtacacc gatctcggtc tgtccgacaa
     9301 aaagctccgg ctgcgcgacc acgaaaagct cgcgcactac tcgaaagcga ccaccgacat
     9361 cgaatacttg ttcccgtttg gctggggcga gctggaaggc atcgccaacc gcaccgactt
     9421 cgacttgcgc cagcaccagc gtggcgttcg caccgtcggc aaatgggatg gcggcgatct
     9481 cgctgcggtt gaattggccg ccgaggatgc cgaataccaa aagggcaagc tgtcgtattt
     9541 cgacgacgaa aagaaacagc gctacgtccc ctacgtgatc gagccggcag ccggtgccga
     9601 tcgcgcgacg ctcgcgtttt tgtgcgacgc ctacgccgaa gacagcgccc ccgatgccaa
     9661 aggccaggtt gaaacgcgcg tcgtgctgcg actccatccg aagctggcgc cgatcaaggc
     9721 tgcgatcctg ccgctgatgc gaaaagacgg ccaccccgag cgcgcgcgca agatatacga
     9781 cgatctgaaa aagcgctttt ttgtcgacta cgatgagagc gccgcaatcg gcaagcgcta
     9841 ccgtcgccag gacgaaatcg gcacgccgat ctgcatcacc gtcgattacg acaccatcaa
     9901 agacggcacc gtcacggttc gcgatcgcga cagcatggag cagattcggg tcagcgagga
     9961 caagctgcgc gggctgctcg aagaaaagct cgggatgtaa aagacgccga ctgccgcggc
    10021 agccgcgtcc ccttcccccc acttccatcg aggagatttc catgaagcgt gcacttttcg
    10081 gctgtctttg ggctgtgatg gcgttgggct gcggcgggac ttccgacggc accgcctgtc
    10141 cggcgatcgc tgcatcgaac tttaccgtca ccgtgaccga cgcggtgact tctgcccgca
    10201 tctgcgacgc caccgtgtcc gccaccgata ccagcaccgg cagcagcatg aacctgatgg
    10261 tcttcggcgg cgcgaccgac tgcgcgtaca gcggcggctt ttacgaacgc cccggcacct
    10321 tcaccctgac cgcgcaaaag agcggctacc ttcccaccac catgccaaac gtcgtcgtta
    10381 ccaaaggcgt ctgcaacgtg acacccgccc aggtctcgct caagcttggc aaatagcgct
    10441 tattccaccg acggatcttc tggcggcaca cccagcgttt cctgaaacat ctgctggtag
    10501 ttttggaagt ggcccggtcc gaccggctgc acaaaaaacg cctcggcgag cggcacctct
    10561 ttcttcagca tcgccacacc ctgcgccatc atctgcagaa agttccgcgc ccccgccgtc
    10621 agctcggcgc gctgcgcatt cgtcagctgc gttggatcgg cgcctttgac aaaccagtcc
    10681 gccagcatct cgatctgcga cgagtgacag agcagcgacc gcactttggt atcaaacgtc
    10741 gtgctgatgt cgacgagccg gttcggtttg tgctcggtcg gcatcatgta ccagacctct
    10801 tgcggctggc gcgccgtcac gccgtccacc aaatgttcgg ggtggagcag cggaaaacac
    10861 gaaaacaccg ctgcttccga cgccattttg cctgccgtga tgtgatcggg atggatctcg
    10921 tagcgcttcc acggatcgaa cgtcaccact ttgtcgacgg cgagcttgcg cagaaagtag
    10981 atcaatcgtt cgcgcaacgc cgctgcatgt tcggccagat gtccgtcggg aaaaccgagc
    11041 atgatcggcg gctggccacc gaatgtcgac agcgccaacc ccagttcttt cgcccgctgc
    11101 tcggcaagct gtgcctgtga aacatcgcga cgtttgctgc caatgtcgcc gttggttgca
    11161 caaatcgcat agacggtgtg cccggcctgc gcccacttgg ccagtgtgcc agcggcaaaa
    11221 aactctccgt cgtcggggtg ggcataaatc gcagcgatga cttggcggtc gctcatgggc
    11281 tgtttccttc cttggcagga taggtcaagc cccatgggcc gcgaaactag ctgcgccgcg
    11341 tctgcaaaag gccgtcgcca agctggctga catcgagcgc ttgcggatca ccgatgaaaa
    11401 tcagccgccg aggcgcccgc gacagcgcgg aatcagaggg cagctcttcc cagtcaattt
    11461 tgtcgccgac cgcctgcacc aacagcggtg tcgtcggtgt tgccgaacct gtgtcatttt
    11521 ggcgatcgac gatccctttg atccgaaaga tgcgatccga aagaccgtcg agaaaatcct
    11581 cgagcgcatt ccagtcgatc gcatacggca ccgaaatggt gaccgcggca atcccatgcg
    11641 ctttgggatg atggccctga tgaatcggcg gcgtcgtcgg cttcgccagg tccggcggta
    11701 gatgcgctga aagatcgatc tggccgagca gcagcctcgc gtcgaccatc gcgtgatcgg
    11761 caaaaacctg ctgcgcccgc ggcgacagcg accgaagcca cgtcgaaagc cgcgtctggg
    11821 tgtcccgatc gggtacaagg tcgattttgt tgaggatcaa aagatccgcc gcgcgcactt
    11881 gatcctcgaa cagatcggga tgctcgcggt gcgctcgctc ggcgttgtgc agatcgatga
    11941 ccgtgaccac ggcgtcgcac tcgacgggca catcaaacga aagcgcattg acggcaaagc
    12001 tgacttccga cgcccgcgca atgccgctcg gttctatcaa gatgtgctcg ggcggtcgcg
    12061 caagtccgtc gccccgcgca agtgctgaaa gcgcttcgcc cagctcactg cgcagcgtgc
    12121 agcagatgca gcccgacaca agctctgtca cccgcagcgt cggcgtctct gtctcgctcg
    12181 cgatgatttc atgatcgagc cccagctcgc cgaggtcgtt gaccagcacc gccagctttt
    12241 gcggcagatt tggatcagaa agcagccgac ggagcagcgt cgttttgcca ctgcccaaaa
    12301 agcccgaaac gacggtgaca gcggttttgc gctcgcccat gtctagttcc cgctgcggct
    12361 agtcgaccac ccgctgtttg gcgttttccg gctcttcgag gtagtagcga cgaccgcggt
    12421 aaaactcgat tgccgacaag cccaccgaca ctgccagtgc gacgtacaaa agcgacgccg
    12481 gctgaccggc cagcaccagc gcgcaaagca gcgtgatcag tccgacccgc gcgccccaca
    12541 gcgctgccga cgcttgcacc cggtggaaac acaacaggtt gtcgagtcca gacacggaaa
    12601 gctcgatcgc catgatcagc atcaccaccc acgggcgcag gacgtgctgg gtctggagga
    12661 tgagaagcag cgtcggcggc acaatcgatc cgaacagccg catccagtcg cccgctcgca
    12721 gctcgccttt gcgcaaaagc ggaaacagcg gcgtgatgta cgtaaagccc gaaatccagg
    12781 tgatgccaat gatcgtcacc gtcagaaact gcgccgtcgc ttccgacccc cagagctgct
    12841 gaatcggcag cagcacaaaa aaccagccac cgaaaatcag cgcgcctttc cacaccacgc
    12901 gacggaccgc gtaacggatc aaaagcgcca ccaccggcag caccgacccg aggatgatcg
    12961 cgtagagaat ccagtgctcc ggcaccatgc gcagaaggaa aatcaccccg ctgcccgcca
    13021 tctgtgccca cgctttgatc ttggccaaga gcggagtttt cggcgaaatc cgtcgctgct
    13081 catacatcga tcgcaagctg gtgatcacgt attcgcgcga aagcaaaagt ccgacctgcc
    13141 acggatacag ccacgggtga tcgtccttca tcagatggat aaagggcaga aagctcacca
    13201 gcacgaacgc tttgtcggcg atcgggtcca tcagccggcc cagctccgtc gagccatacc
    13261 gacgcgccag ccatccgtcg atgaaatcgg tacagccgag gattgccata aacagaagcg
    13321 ccagccactt ttccgtcgga ttgccgtaaa gaagatacgg tcccagcggc aaaagcacga
    13381 tccgcagcag cgtgacctga ttggctgtaa tcggtggttt gcccatcgtg aagtctccca
    13441 aagagcggat cagtactggt agcgcaggcc aaactggacg cggaatggca cctgccggct
    13501 ggttactgcg ccatacgccg cgctgttgcg tgcttcaaac gcgggctggt acgtgctcgc
    13561 tgatgtcgtg ttcgtgcggg tcgccgaaga caggtccagc acgttgaaaa tgtcgacgat
    13621 cagcgtcagg tgttggcgca aaagctcata ggtgtcgtac tgggcgcgaa ccgacaggtc
    13681 cagcacgtcg gagctgcgca gctcggtcca tttgcgaatg tcgtttgggt cggcacccgg
    13741 atcaatgccg cgaaagccgt agcgaccttg cagtccgaac gcggtcgtcg caaacagccg
    13801 cgtcgtcggg gccccggtga catacgccag gtttacgccc gccgtcagac catggaacgt
    13861 gtagctggca ttggctttga cctgatggcg atgatcgtcg agcagatagc cgtacagccg
    13921 cagatcgcgc ggcggatcat tgcgcagcac cgagatttga tcgtcgagcg tgccgtccaa
    13981 aaacgacaac gtataggcga caaacacgct ccaggcgggc gacggactgc cggctatcgc
    14041 aatgtccatg gcttggtagc gccggaacgc ttcgcccgtc ggacgataga cgcggacgtt
    14101 gccgcgcgtc ggatcggaaa accctgcgat gttgccaccg tcgagcgtgc gataggcatt
    14161 gatctcgatg tcttcccaca ggtcttcgag cttccgatac gtataggtga cgctgccgat
    14221 cacgttcgcg tacagctcgc gctcaaatga agccgtcaca tagtccgagt gcggaggtcg
    14281 caagctcagc ttggcattgc cacactctgg gcgaatctgt ccgtctgcgc agcgaccgga
    14341 aagctcataa ccgcccccgc cgccatcgga cgaaaagaaa cgatcgaacc gcttggtcgc
    14401 cccgttatag ccccacgtcg tcgacattgg gtttacgtcc gccagcgctg cactgcgcag
    14461 cgaagcgact tcgtttgcgc gaccgtaggc aaacttcacc attgttttgc cgtcgcgggt
    14521 gagatcaacc gacgccccga gtcgcggccc aaaacccaac aggttctgcg tcacctgacc
    14581 ctgcgagttt tccgccgttc catagtcgac gcgaatgccg ggaatcagcg tcagccagct
    14641 cgtcggtttc caggtgtctt gcaaaaacgc gcccagcgcc cagccgaccc gcacttgcgc
    14701 ccgcatcggt tcgtaaagcg agcgctggaa acacggtgtc gccgtcgacc cgagcttggg
    14761 ctgcggctga ccttcaacac agcccaatgg cctgtcgagc gaggtcggat cgcgcccaag
    14821 caccccgccg ccctggcttg tatcgtcggt gtatatctgg tttcccggtg tgccggtcag
    14881 cgcatagttt cgcaaaaact ggaactgcgc gccggccttg atcgtgtgct gacctaggcc
    14941 accgcgctgt atccacgtca tcgtcggatc gaactggaat cgcagccgcc cgtcgctgac
    15001 gatcgctccc gcatccgcgt tgttccaggt gatcgtgctc gcgttgtcga tgtgcgtcgg
    15061 gctgaccaga tcgccaagct gcggtcctgt cgtaaaacca aaccactgaa ggccggtctg
    15121 cacgccaaac agcaggttgt ccgtcgcgaa gtaatcccag gtcaggatgc cgaagacgcc
    15181 gccttggttt tgaaaatagt cagcctcggc ggtgtaggcg ttcgtctggc tgccatcgat
    15241 gttgcgaatg ttggtaagcc acgcaggatc ggcgttgacc gacaggttca gccggtgccg
    15301 cgacgacggc gcccacgtca gcttcagccg cgtatacaga tcatgtcgct cgagcggcgg
    15361 gtgctggctg ttgagcggcg cccccggaat caccgagttt atgcgatagc ggtactccgt
    15421 cgaaaaatag aaccacagct tctgctttac gatcggcccg ccgatgttca ggtttgcctg
    15481 atagctggcg acgggcgggc ttggatcgct ctgattgagc agccgccctt cgtaaagctg
    15541 attgccagcc cgtccgccga gcgacagcga ctggtggttc acataaaagc tcgaatcgat
    15601 cgacagcttg tcgctgccgc tcttggtcat caggttgatg acaccgccga gcgagttgta
    15661 ctgcgcgtcg accgccagaa gcagaaggtc catctgcccg atggcatcga agttgaaatt
    15721 tgccgaaaac gtcccgtcca ctgggtcggt gatgtccaga ccatcgacca gatagcggtt
    15781 gtgacgctgc gttgcgcccg ccatcaccgg attggagctg cccgtcacgc ccggaagctg
    15841 ctgcaccacg tcctgatatc gtcgtggtgt cgcaagcttg gcctgtgatt ccgcggtcag
    15901 gactttgcct gtcgacaccc gatcggaatc gagccgcttt ttttcttcga tgatcgtctg
    15961 cgtttcggct ttcgccagct ccgccgacag cgggatcttc aggttttggg tctgtcccag
    16021 ctctagcgac acccgccttt ccagcgcttt gagaccctca tattcgacgg tcacggtgta
    16081 cttgccgggt ggcagaagcg ggaaatcgaa gcggccttct tcatcagccg tggtttttcg
    16141 cggtccgccg atcagtgaat cgccgcgaat cgtcaccagc gccccttgca tcgggacatc
    16201 ggttccgcct tcgacgacgg ttccgcgcag ccgagcggtt tgttctccga cggcccaggc
    16261 gcgatgcacg ccagaaggga acatcgaacc agaaagcacc aggcccgcca aaagtagctt
    16321 gggcaaggtg ccgacacggt gacaagctgc ccgacgagga gcagtgagcg gatgcgatct
    16381 ttgcatggtc tttgtccgca aagtttgcgc accttactac acgccgcagc gtcgattcca
    16441 gcgtggcgca catggaaaca cgacgaaaac gggaaaggct ttgggggctg ccagcgcatc
    16501 gcgcatcatg tggacagcct ttttgacgct aggaggagcg agagatgacg accccgattc
    16561 acatcccaca tcggcatttg gggcggtgtg gtctcaaagt ttcggcgctg acgctgggcg
    16621 cgatgacatt tggcgaatcc gaaacgttca tgcgcggtgt cacgtcgagc gatgacgaag
    16681 cccgccgtgt actcgatgcc gctctggatg ccggcattga caccgtagat accgccaacg
    16741 tctacagcga aggccgcagc gagcagctct tgggtgagtg gctgcatggt cgtcgcgaca
    16801 aagtcacgct gctgaccaag tgccgtttcc cgattggctt tggtacccgt ccgatcggtc
    16861 cgcacgacca agggctgtcc cgcaagcaca tcgtgcaggc ctgcgaagac tcgctgcgtc
    16921 gcctgcgcac cgactacatc gatctgtacc aagtccacat gcaagaccgc aacgtgccga
    16981 tcgaggagac gctgcgtgcg ctcgacgatc tggtccgtga tggcaaggtt cgctacgtcg
    17041 gctgctccaa cttcaccggc tatcgcttga ccgaatcgct gtgggccgct gacaagcgcg
    17101 acaccgtccg ttttgaagcg atgcagattc agtggagcct gatcgtccgc gacgccgagc
    17161 gtgaactggt tcctgccgcc aagcactttg gcatgggcat cctggtctgg agcccgcttg
    17221 gccgcggctt tttgtcgggc aagtatcaga agggcgaagc gccacccgaa ggtgcacgcc
    17281 ttgccgcatg gaaagaatcc tacggcgcga tgaacaccga acagaactgg gccatcctcg
    17341 acgtggtacg tgacgttgcg cgcgaggtcg gcagcacgcc cgccgccgtg tcgctggcct
    17401 acctgctcgc gcaccccgag tgctcgtcga tcatcattgg cgcccgcaat ctgtcccagc
    17461 tacaggacaa cctggccgcc atgtcggtca cgctgtcgcc cgatcaagtc cgtcggctag
    17521 agcaagtctc gcgtccgcgc tttggctatc cctacgactt tatcggcctg cgcgagccct
    17581 ggtagtccgt cgcccagcga cgctgatgat cccggccaga tccgccgccc gatccgctcg
    17641 cagatccaaa aaatcgctgt cgcggacagc caaatgaaac accaataaag ccggtagctc
    17701 gggaaatacg cttccgagcc cggatagata aactgcgaca cattcacatt ggcctcatgc
    17761 ggtgaaacat agcggctcac cgtctgcaca agcacgccca ggcccgtcgc atacacaaag
    17821 cttcccgccg gaaagccggt ccttccaagc caaaccagcg ccaccagaag tccgcccagg
    17881 tgcgtcagcg ccgatgtcaa aagaaagtcc ccggtgcggc acgcatcgaa gatccacagc
    17941 accgtcccaa gcggcagcca cagcgccgct gctgccgtcc atctgggcga tcgggccaag
    18001 atgcccaccc cgacgacaaa acaggacacg gtacacagcc acaatagctc gtaggcatgc
    18061 ccggacagca catgccggct cccgtggatc gcaaaaaacg acaggggcaa aagtcccagc
    18121 cagcgcagac cgcccagatc gatttgctgc tgcccgcgtt gcggaggatc ggaggtcact
    18181 cgatgtttat ccggtccatt tggctgtggt tgtttgcgct gtcgctgttt gctgcggttc
    18241 ccgcatctgc cgaccctggc tttgtgccgc ttcccgtgtc cgccggggca ccgcgggcac
    18301 tggaaattca ggtccagcgt tacgagggtt ccaccaacgg tcagctcacc gtttcgattc
    18361 gcaaccccac cgatcgggcc gtcaagtttg aagctggcgg cctgtacttt gtccccgaac
    18421 aaaacgccaa cgatgcaccc cagcggcttg gcgcagccgg gccctttgcg gtgcaaaagc
    18481 agtcgggcgg ttttcagccg agcgacggct acaccctgtc accaaacagc caggtggaag
    18541 tgaagctgga tgtctactgc attgactcgc accgcagaag cccgcggacc ggcgatgtct
    18601 ttcgggtcgc tgtcacccgc atgcccgccg acctgacccg ctcgatccaa tccgacacga
    18661 gcaccgccgc ccgccaaaac ggtggctacg cagcccccgc cgccaagtcc gccgtccagt
    18721 ccgagatttg gaaaaaccgc aacaagaagt ggatcgagct agacggcgaa ggcgtccagg
    18781 aaaagcgcaa agacgcgccc gcgccgcaga tgcagcacat tcagaagcgc tacattgacc
    18841 acaactagta gccgttgcgg cgaatcactt tgtagctggt ccccgccgtg cgcgatccgt
    18901 ggacgatgcc gtagctgcaa cccttggcct cgtaggcttt catccagccc cacgggtcgc
    18961 cgttgtccat catgaagatg tggccggcgc cgttttcgtt gtagacaaac gcatcgcctt
    19021 gcttcgctga tccgcgcgcc accgtccgcc actcgcgcgt ttcgtttgca aagttgtacg
    19081 tcgaatacgg atggctgcac gtcgacaccg agcttgtccc cggaacctgc cacaccttgg
    19141 cgacgtatcc cgagcagtcg gcgccataac tgccaccgtg cgtacatccg gggcacgatc
    19201 cggtgcagct tcccttgtct gacgatccgg gtttccagca gcctccgccc caccaatagg
    19261 aaaagccgac acctgattcc gcccgtgaaa cggcgtcggc cacccccccc gctgcgctgt
    19321 acggcacaag gtagtcgccc gagcagtagc cggtgtaact gccccaggtc actcgataaa
    19381 agccgctcga ttccgacagc acatcaacga tcgtgccttt cggcatgctc gcgatgaccg
    19441 cataggatgt gccggggcca gatcgcaggt tcaagctgct cgctgtgacc tcggcttttt
    19501 cgcccacgcg cgccgacgct gcatgaaagt cggtgttttc cgcgtcggcg acgccatcaa
    19561 tgccggcgtg gtctgcgccc gctgacagct cgctgccgca gcccgacaac catcctacgg
    19621 taagaagccc aattcccagc gaacctagca ccttgcgcat cacagcctcc acgtccatct
    19681 gcgtccgaac ccgtgcaagc cgcttgccac cacggctggc ccggactttg ggcgaactat
    19741 cgcaaacgcg gcgtggaagt acgcgaaaat ctgggggttt tgtccccact ctgtagtgtc
    19801 cagcccgcaa cacacatcga aagcagacca agtaggcgca gcaaaactcg gtagaagtgc
    19861 ttctttctcg acgctggcgg ctgatataat gagcgagcca cagcatgtcg agcaggcgat
    19921 tgaacagtct ttgggggctg ctcttgctgg tgacactcgg gggaacgcag gcgtggggag
    19981 gcgatcggca cggtcggctc tctgcacctg tgcatcgtcc gacgctgtcg ccaggacagg
    20041 gcttgcgccc ggtttccgga agccgggaaa ctttccggct caaaaagcca cagcctttgg
    20101 gggcgacgcg gcgtccgctg gtcgcgcggc ctcctgtgcc gagcgtcgca aagccgatgg
    20161 cgaagccgcc gcttgggggc catctgccgg tcgtgccaaa gccgctacct gtcctgcccc
    20221 atgcacctgt gcttggcaag cagccgccac ccggtccgcc cgaatatgag ccgccacccg
    20281 ccggggaggt cgcggtccgc tgggccatcc ccaagcggcg tgatctgggc gtcaagatcc
    20341 acactgcccc cagctattcg gccaatgtca tcggcgagct gcggggtcaa tcggcgatgc
    20401 tggtcggtca gcagatcgct gccaaggtgg gtctgtgccg cacttggctg tcggcgctgc
    20461 cgagcggatt tgtctgcgaa agcgatgtca tcgtcgaacc tgggtatctg tcgagcccac
    20521 cagcgcagga aaaaagcacc ggctggcagc gactccgtta cggcgtcgtt tcctcgttta
    20581 tgaccaagct gcgcggcggc ggcagccact atttgcgcca agttctccac aaaggcgacg
    20641 gcgttaccat cacccaggaa aaaggcgaag aggtccagat catcggccaa aagtggctgc
    20701 ccaggcgcga tgtccaggtg ttcgatccgc cggtcgcgcc gccggtcgcg ctcgacactg
    20761 tgccgccagg gctgcgcctt ggctgggccg ttccgcccat ggatgaagaa ggcgtgccgg
    20821 tttttctgcc caagtcagac agcggaccgg tcgcctactt gccgcgctac gccgtcgttt
    20881 atgtcgtgga aaacagcgac agcacgccgg gccgacttca ggtcgtattt cccgaaagca
    20941 ctcgcgcggt gctgcgggcg cccggttcca gccgtcccgc ctgggtgtct tcgccgcagt
    21001 ttgaaatcga ggtcggtcag ctccgccgca tgacgacggc agcgccgccc tccgatctgc
    21061 ttcccgatga gcgctggatc gacatccatc ttttggagca ggtcgcgacc gcataccagg
    21121 gcaaaaaacc gctgtttgcc tcgctggtgt cgacgggtct ccacggcgca acgccgcccg
    21181 gatcgttttt catctatcgc aagtacctga cccagacgat ggccaacctg gccggggccg
    21241 cttcgcagta cgactttcgc gaggtgccac atgctcagtt tttcaacgga cggatcggcc
    21301 tgcatgccgt gctctggcac gatcgcctgg gccacccggt cagtcatggc tgcgtgaacc
    21361 tgagtccccc tgcagcggaa cagttctttg ccttcacttc gccggatttg cccgtcggtt
    21421 ggcactcggt cacaaccttc gcgcaccagg gcgggccacg cagtgcccac ggaacccgcg
    21481 tcgtcgttcg caagtaaccc cgcacgactt cgttgacagt cggtccgtcg gggcgtaatc
    21541 actctttttg acatgaacaa agctgcactc gagattttgc gtcgtggcaa gacgctggcg
    21601 tattcggcga tgacatccga aaccggcaac tttctgccgc cggttcgtta tgcgaaaaaa
    21661 gccgctggct tcgtcaacga tctgctcggg cgaccgctgg cttcttcggc agaactcgat
    21721 cggcgcgacg aagaatcaca gcgtctggat gcgctggctg aaaaagcccg acgcggcgaa
    21781 ggtgcggtgg ccaaaaaaga agcggccccc gtcgtcctct acgtcaccga tcagttcgtg
    21841 cgcgagcgaa aacggatcga agacgttctc aaaggccgcg gcatcccgta tcgcgtgctc
    21901 gatttgacgg atgacgaatc gacgcgaagc tgggcgctga ccaaagcgca agccaccgag
    21961 tttccgctcc tgtttgttgc gggtgaatcg gttggcggct tcgagcaggt cttgtcgctc
    22021 gatgccacag gcggcttgac caaaaaagtg ttcggctagc ggagccgccc gtggaacttc
    22081 gcaccctaca cctcgatggt caaacgctgc gaacgcgggt ctatgcgacc catcccgacc
    22141 gggttgtgga cgttgagctt tcctcggatg cgacggcccg tctgctgaca gccgcggcgc
    22201 cgtttttgaa ctggcttgcc acggcgggct atctgcccca aggcgcgtcg ctcgaagcgc
    22261 tgtggctcga
//
LOCUS       DAORGN010000068        22181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_931_length_22181_cov_6.426647, whole genome shotgun sequence.
ACCESSION   DAORGN010000068 DAORGN010000000
VERSION     DAORGN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22181
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_22181_cov_6.426647"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKL17_09625"
     CDS             <1..193
                     /locus_tag="PKL17_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45031.1"
                     /translation="GTIPTVDATGGHMGVTVDSPSTAVYHYHVNQQTSTTAGTAGQMQ
                     WFITKGSYHGTPAPCSTCM"
     gene            complement(205..1296)
                     /locus_tag="PKL17_09630"
     CDS             complement(205..1296)
                     /locus_tag="PKL17_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45032.1"
                     /translation="MVWRAFFALLLLVGSGGCFQLGPATLGVTQRDFNEAIVRAQNEQ
                     LLLNLVRLRYRDNPYFVEVSGITSQQSIGGSAGIGADFSVSADAPLRSVIRPSVGMTY
                     SQTPTVVLSPLAGEAFVRKLISPLSQEALLHLLRSGWSIARVLTLCLDRVGSLRNAPS
                     AAGPTPMQAPTYADFQKFATDLRMLQQADLLNLSYENAKEQPSLILEVRGLSDHSEPL
                     DRLRKLLGLSKDGVRYTLTQDIRRTGKDVVPLRLRSVRSILFFLAHGVDVPKVHSLAG
                     LVTTTRYPDGRSFNWSDLLGGLFHVRTRRDEPMAAYVKIRYRDHYYYIADNDLESKST
                     FMLLTEIFNMQAGQGVALMPTLTLPVGGR"
     gene            1330..2661
                     /gene="hemL"
                     /locus_tag="PKL17_09635"
     CDS             1330..2661
                     /gene="hemL"
                     /locus_tag="PKL17_09635"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNK45033.1"
                     /translation="MSVKSQTPAEVSRRIRSQELFARAQKTIPGGVNSPVRAFRGVGG
                     DPVFFAKAKGAYLWDVDGNSYVDYVGSWGPMILGHAQPQVLAAITAAAADGLSFGAPT
                     EREVRFAELLTQRVPSMDKVRLVSSGTEATMSALRVARGATGRSKIVKIDGAYHGHAD
                     SLLVAAGSGAATLGLPGSAGVTEGAARDTLTVPWNDEAAIEAVFRDHRSADGQSEIAA
                     VIIEPVCGNMGCVAPEPGYLEHLRKLTADAGTVLIFDEVMTGFRVSHGGAQKRYGITP
                     DMTCLGKIVGGGLPAGAYGGKREIMDKVAPVGPVYQAGTLSGNPLAVAAGMTLLELLD
                     APGIYEQLEKQTARLCHELLALCQQAGIPAVVNQVGSMFTLFFTAQPVKNYADAKRSD
                     TARFARFFHGMLERGIYLPPSQFEAAFVSSSHGDEEIEKTIAAAREVIATL"
     gene            2783..3253
                     /locus_tag="PKL17_09640"
     CDS             2783..3253
                     /locus_tag="PKL17_09640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012712611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNK45034.1"
                     /translation="MLNASEKAPNFTLLDGARKAVSLSDFAGKTVVLAFFPAAFTGVC
                     TKELCTFRDSLAAFNDVGASVLGICVDAPFSNAAFAEKNGLNFPILSDYDRKAVRAFG
                     IAHDDFAGMAGYTAAKRSVFVIDGSGTIRYSWVAPNPGVEPNYDEVKAAVAAAR"
     gene            complement(3250..3723)
                     /locus_tag="PKL17_09645"
     CDS             complement(3250..3723)
                     /locus_tag="PKL17_09645"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNK45035.1"
                     /translation="MTDLDYRDAQIVSLAARVRELEEREATTEGRFQALVQSLDVGVI
                     VLLANQQITLCNPAARELLGGRVGSLAGCSDDDARSGATNPSGVPLLGCDALLREATT
                     RKRPVRDVIVTASQDSDAAPRRLLVCIIPQHDDKGDLTQVIVTLTDVSLCHKLTN"
     gene            complement(3801..7757)
                     /locus_tag="PKL17_09650"
     CDS             complement(3801..7757)
                     /locus_tag="PKL17_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45036.1"
                     /translation="MLQELAPGSLVHGRFEVERLTGSGGMGAVYRAMDRTTMQPVAIK
                     VLFDGADEARFHREALLLAELRHPGIVGYVAHGRTDAGHPFLAMQWLSGIDLAERLRH
                     GGLNLKESLLLIQRVAEALSIAHKRGIIHRDIKPSNLFLVDGDPGKVVLLDFGIARRG
                     LGVRSAAMTRTGAIIGTPEYMAPEQARGQSQLSAAADVFSLGCVFYECLAGSPPFVAA
                     HVAAVLAKILFEDAPKLRTVRPELPLPLEDLLLRMLHKDPKLRPADGGAVLAALLLLP
                     TLPTLQGPAFEHAVVGPSIRGSEQQLVSVLLVSDVLEVSSQQTLAEADDAAQKARLEA
                     LRAHLAPSGLHLEQLVDGSLLGTMHQLGSGAATDLAGRAARAALHIHERWSEAVVAIA
                     TGKGMLAGDLPVGEAVDRAAVLLRRRTTAGGTHAVLIDELTAGLLDARFEVVRLSHGA
                     FALLGERLSIDESRKLLGKPTPCVGREQELLALGAVLGTVIEESTPRVALVVAPAGVG
                     KSRLRHEFLRRTSAGATPVQVLLGRGDAMNPGAPFGLWSAVLRGLCAEVRGQGAPDGF
                     GQMLALATHAVGEREAARVAAFLAELAGMSPPLDKYPPLHAARSDGRLFLEQATLAVT
                     DFLRGRCADQPVVLVFEDMQWGDPQSIELTESALRSLPDSPLFVLVLARPEYESRLPK
                     LGKDRGLQEFYLGGLGKRASERLVQNILGAKAPASSVARIVEQAAGNALYLEELIRAF
                     AEGKTEHLPDTVLAMLQARVGRLPPEQRRVLRAASIFGDVFFRGGVMALLGHGSGSGT
                     GDHIDQVLRELSDAEIIERRRDSRLPDDVEFGIRHSLMREAAYSLLTDEDRTLGHRLC
                     AEYLQRSGEPDHAVLARHFELGSDGPCAVRHYVEAAIDSWKRGGAERTQELCLAARRV
                     GAQGEWLGAVRCLDGQMALFAGRLSDSLQAAREGILLSPPGSRFYYWSILGLLATATL
                     AGKMEVTMEVLRDFDGKLPQPDAAWYFQEAASLVAQLYAFLAMREPALRTISQMRAVC
                     DLYESDPQQPLYVAAWQRAQAAMDLYLDDDMFRTQALLAESADTYLRFGQVRLASLVT
                     FDAALNSMPLGDWEETQRLGRQMQKYATTLGDSHLLKSAEMMIGAGDLMLGDETVRRA
                     QRQMLLDVVDSSGEDRVRQGEAISILAANYLYCGEWEEAVQHFRQAIDVLGALPTVVV
                     YPYSCLTTALLRLGRISEATVTVEAGLSVLALMDDHGPYAASMWLSKAEVELALGHDE
                     QGRVALRRAVRLLRHRLETARTQRQREFLLRGVPQHQRVVKLCEQILGEPFERSSN"
     gene            complement(7845..9416)
                     /locus_tag="PKL17_09655"
     CDS             complement(7845..9416)
                     /locus_tag="PKL17_09655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNK45037.1"
                     /translation="MISTSLVLIILITGLLGTSSTYYGRRAFDEAAQKLSESYDSALG
                     ARAQTLTAAMSESARAALMQSDYSSLKTFVPKAAKTDPDVKALYVISDTGYVAAHSDE
                     KWNDAEKKITEVDPVLGPELEKLTKPLEKTIDVDEGGQKDTRRVFARPVATEQGKKFG
                     TLVVIYSMASLKNQLRSIEEYKLAQGQAFVVQVAVLGLVFLAFAAGISIFQSLRITRP
                     IQQLAQRADQIARGDLTTRVEIQSPDEIGLLAENFNFMADRLQVLLEETAAKAVLEKE
                     LELARTIQETLVPTSEVIQRGPIQVVGHFQPATQCGGDWWAVYDLPGDRVLLIIGDVT
                     GHGVPAAMITAAAKAACDVGRALEGERITSSRILEIMNHAIYESARRKFVMTCFACVI
                     DQKTRGITYANAGHNFPYLYRQPSSLKEGESPFTVLMSRGNRLGDVPESTFAENNQQL
                     RPGDKIVFFTDGLIECENPMNEEFGEKRLRQVIGQTGELAPAAMRDALMAQAAQFYAD
                     KPRKDDITLLIAQIS"
     gene            9509..10063
                     /locus_tag="PKL17_09660"
     CDS             9509..10063
                     /locus_tag="PKL17_09660"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNK45038.1"
                     /translation="MHPALERFIQQFKATSDVVIDSWFICDSERNIVDYNRAFFSMFP
                     RPEARQLKVKKCLDVMRLTICQDRCIAQQCWREGRQVRLDEIDGKPVGQPDDAPPRRY
                     VLSAIPILDENNKPVGALEIQRDVTDEALVQDKYKKMLENEARERERLATQIRSRTRE
                     LYEANQLLLKAQAELTQQKKGLAF"
     gene            10078..11505
                     /locus_tag="PKL17_09665"
     CDS             10078..11505
                     /locus_tag="PKL17_09665"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNK45039.1"
                     /translation="MGDKSFTEAVCGLGLGDEGKGSFVDAWVGRMQTPALVVRYNGGP
                     QAAHHVVLPDGRWHCFAQLGAGMLQNQTETLLSPYMLVEPLSLVREATHLAELGVGTP
                     LLRTWVARRCVVVTPFHKLLGRLREVARGNARHGSCGLGVSQAWLDSQKPGLPSLRID
                     DLLDPGTLCSKLRFLQLVKVDQAEQLLDSLPPQQRTADAQRLLTELQHRDVPQKLADD
                     YARFVSSGVHFVDDQQVQSMIAKPQRPCILEGAQGVLLDAEHGFFPFVTPSKTSLFQA
                     NLLLNGRPSRQVGIVRAYATRHGPGPLVSEDAELTRVLRETHNVENPWQGPMRVGWFD
                     AVATRYALAVVSGQVHIALTCLDRLVGLSSLYLCNRYRYDGPWAADLDDFFVFDAANH
                     VSDLRRAAQPTLARQQRLTELLAHCRPILQTLAPIPTLLQAKGSLSTAADAYIDALLA
                     AIKLDRNALICLSVGDKASDKRWFD"
     gene            complement(11518..13179)
                     /locus_tag="PKL17_09670"
     CDS             complement(11518..13179)
                     /locus_tag="PKL17_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK45040.1"
                     /translation="MRDDEPQRPPVSTTAWRRVHSHLPAKVDVAVIGSGPGGLVAAAL
                     LAQAGKKVALFESHYVAGGCATQFRRGPKHARYHFDVGLHYIGDCDRQGTIPRILDEC
                     SVRVDYVPLEQDGFDTLIYPDFRFRIPASLDLYQKRLCELFPSQRVPIERYVKLLSSV
                     GKAMRAIEFSDRPKLRTLLPLAVDALRLQGHRRSTASEMFDAISVSDPKLRAVLLGQN
                     GDYGLPPSQVSAMMHLGLSAHYFRGAFYPSGGGQVIADRLAARIESLGGRICLRHTIE
                     KILIEDGRAVGVQIAARDEDPVRQVRADVVLSNADFKRTLIDLVGPEHLPSEWLTRAR
                     ESKMAAALFMTFLGVKGDLRQDGMRATNYWQYDHYDVDAMYKLSATHKTELPEVQAAY
                     ITSASVKDPEHWLHHAPVGVTNVEVMTIVPGAPSRWGISDADVLGWDYRKSEKYQDLK
                     LQIEDNLIGRLDKLFPGTAARICYRESATPLSHSRFTRASDGSGYGLAATPDQFLDKR
                     PGYRSPIDGLFLCGASTRAGHGIAGAMMSGWKAAQRILGSDDTRR"
     gene            complement(13207..15363)
                     /locus_tag="PKL17_09675"
     CDS             complement(13207..15363)
                     /locus_tag="PKL17_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNK45041.1"
                     /translation="MSSIGRSESISRLDQLKVSSYFGCDTFGWRTMQERLPKEVFRSL
                     RKCIRKGERLSAEVAHVVAQAMKDWAIERGATHFTHWFQPMTGATAEKHDAFITWDDH
                     GGLIESFSGAQLIQGEPDASSFPSGGLRTTFEARGYTAWDPVSPAFLMAGPLGKTLCI
                     PTAFIGYHGEALDHKVPLLRAMDFVGQQAAQALSLFGQDGAVVTAQCGAEQEYFLVDL
                     ELYKARPDLMFCNRTLQGARPPKGQELEDHYFGAIKERVLRFMQDLELELFRLGIPAK
                     TRHNEVAPNQFELAPIYEPANVAADHNQLLMEVMKKVGERHDLAVLLGEKPFAGVNGS
                     GKHVNVSLGSDDGQNLFDPGHSPEQNLQFLYFLSSVLLGVWRHGAVLRASVASASNDH
                     RLGANEAPPAIMSVFLGEQLNRILDQVESGAVVSSSERKQLDLGIPALPVLAKDYTDR
                     NRTSPMAFTGNKFEFRAVGAGQAIAVPLSVIQTIIGDALSWMNQRVREVEPKFPGDRQ
                     QAIMEVIREVVATTRAVRFDGNGYSLQWRDEAARRGLPHARSTVEALSAWEDSKVQDL
                     FERAGVMSKPEQEARSHVRHEQYVKSLQIEAQVLREMAETQILPVILEDVKQKAACLT
                     QLQAAGITTPDPVRAAIKVEAELLEQAYQRLLALKATMAEADATDDLHKRTAAFARDV
                     LPACQVLRESLDGLEDQCDARLWPLPKYRELLAPLG"
     gene            15457..17610
                     /locus_tag="PKL17_09680"
     CDS             15457..17610
                     /locus_tag="PKL17_09680"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNK45042.1"
                     /translation="MTQKTNPALGAQQLSRRGFLYTVVATTASALLLPQTGCETAPPQ
                     MMMADGKHFFPQSVASGDPRADSVVIWARALNPASPAEPCQVKLQVAADTSFLTVVYD
                     GMPINLTTDQDHTVRIKVTGLSARTTYFYRFLVLRDDVTLTSQVGRTRTAPSADDKVP
                     VKFAFWSCQDFEGRYYNAWQRLLSLDEDLDFVLGLGDYIYETAPATPTSTPRSVGFSD
                     PSGAILVGTSRIAASTVGQYRDLYKRYRSDAVLQKIHERYPMIVIWDDHEYSNDCWGA
                     TSTYQNGKTSEVNGTRRRNAERAFFEYMPIDDSSPMSGQLTVADSSLYPRTRLFRDFH
                     FGQNLHLVLTDYRSFRPDHLIPEEAYPGSAALDASALRALGAYPIFEFDDGFAAIDID
                     APLYARQKQVLTQAAIAGCLAAGLAQADAQQKAASWVTGNLSLLYVNAVLKATNQTAL
                     IVAPSRDMDRAVSWLHLGKQQLFSPQGSRYLAVKPLFELYAQYQWQLSQHESENVFGK
                     DQQEWLQKTLTESKTKFRVIGSSVMPTEGIFNLTTTPGLPAAYQNVFVYGLDGWDGFP
                     NKRQELLDFLKTNNVGNTFFVAGDIHGGFVSVLDGTAPALTTPAISSGTLQESIAESA
                     LALGFTLDSPAYAKVVANLDKTLQEGNPSIAFSASDQHGFVIVEVGETEAQATFHLIS
                     QAEVKTDSTSLPAAALAMKFATQKFKLSGATVTKL"
     gene            complement(17632..19716)
                     /locus_tag="PKL17_09685"
     CDS             complement(17632..19716)
                     /locus_tag="PKL17_09685"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNK45043.1"
                     /translation="MRNYSEAEIRDVVVIGHGGSGKTSLGEAILFDAKLVTRLGRVDD
                     ETSNLDSEPEEKKRKGTINPHVTAVEWQKGKLNLIDTSGQGDFVVDTMIAIGAADSAL
                     CVVSASDGVQVYTEKTWEEADALHMPRMIFVNKMERERADFAVVLDQIHRVLSPQAVA
                     VQLPIGSESSFEGMIDLVAQKAYRFSEDGRDVTVSDVPASLAAATKEARDKLIEEVAG
                     SDDDLMAAYFDHGTLTDEELQLGLQRAVAAGKLFPVFCGSAAQNRGVQPLLDAMVAWF
                     PPASAGRTRTGSGKSGSEVRRSPKSSEPLSAYVFKIIPTEVGKMALVRIVSGKIAADS
                     PIWNASHDKGERVGQLYVFTPGKRTSVPEAHAGDIVGLAKLKLTRIGDTFSDEKELVK
                     FAAPTVPAPVVRYSLHLKGKTDEAKLAQKLHDIHEEDLSLSVEYDPATKETLIGGCGP
                     VHIEATLDKLRRVGVEVELAPPRVPYLETIRGRVKNVEGKHKKQTGGKGQFGVCYLDL
                     DPAPRGTGLVFEDAIVGGSIPRQFIPSVEKGIKERMVRGGLAGYPITDVKVRLFDGKY
                     HDVDSDSRSFEMAGSKGFLAAFKQASPVLLEPYMKIEVSIPEEYMGSIIGDLNNRRAH
                     IAGMEAKGKYQIVIAQIPMSETLDYSAFLRSATGGRGSFSLATSHYAEMPQQLADKVI
                     ASAKKHVEEEEE"
     gene            complement(19807..>22181)
                     /locus_tag="PKL17_09690"
     CDS             complement(19807..>22181)
                     /locus_tag="PKL17_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45044.1"
                     /translation="ALAIIIGSSVYSFTLILLAFLIGLAAGAAIITRLLPRIARPMLW
                     LGSLHALTLVAVGYSYLMFDRLPGIFLAILRSGNLGIDELLSTQFGLSALAILPATLA
                     MGGVMPLTMRIYTGSASGAGHDVGIAYALNTLGAIVGSFAAGFLVLPGLGLQRGLLGS
                     ALVTAGLAAMLILVAETQKPGKATGLDAFDPSPQVPNASRRRLYLTAACVMLAAALFA
                     VLSPRWNLTHFAAGLFRVSVARSVVRLHNRVLPDLLYYKDGIATTVSVERWGKTIALK
                     NNGKVDASNSDDMGTQIMVGLLPLLWHKTALDKPPRVAVVGYGSGVTIGAVTQFPIGH
                     ADVVELEPAVVEAGDRYFAEVSHLPSKDPRVRVIIGDGRNFMTQAQGPDDRYDVIVSE
                     PSNPWITGVSNLFTIDYWKLAKSRLADDGVFCQWAQLYELSPVNIKTLLRSFATVFPY
                     TYVFTSEYGSSDMILVATKQPMSLDLNRLRRNFQVPSLRAELSRAGIDRPEEVLADLL
                     LTPDEIWAFSVGGHINTDDNAKIEFAAPRDLLGYMRHESSVPRVHSDDWIYGRFERYL
                     IGHGMPFDKPPAWLPDDGSGVRRADLYAALGRALLMRGKRKASARLLNRSEGIGGGAQ
                     TDELSQLLTLFGQRAATEHEEALSENWDGSDEHFADDLGTPTLPVGLTQTEQATILRQ
                     WSDAKRYLNRREFVPALRELRDWPMKWIDQMGPSVRLLLGYLLYKADLSPEALDHLAE
                     LSDDEEYVKRHPGVYYYLARTQMQSGKPRQAVHNMEQYVKKSPTARGLRR"
BASE COUNT         4346 a         7197 c         6411 g         4227 t
ORIGIN      
        1 tggcacgatt ccgacggtgg acgcgaccgg cggacacatg ggcgtcaccg tcgacagccc
       61 gtcaaccgcg gtctatcact accacgtcaa ccaacagacg agcacgacgg ctggcaccgc
      121 cggccaaatg cagtggttta tcaccaaagg cagctatcac ggcacgccag cgccctgctc
      181 gacctgcatg tagaggccta atcgtcaccg gccaccgacg ggcaacgtca gcgtcggcat
      241 gagtgcgacg ccttggccgg cctgcatgtt gaaaatctcg gtgagcagca taaacgtgct
      301 cttcgactcc aggtcgttgt cggcgatgta gtagtagtgg tcgcggtaac gaatcttgac
      361 gtaggcagcc atcggctcgt cgcgacgggt ccgcacatgg aacagcccgc ccaaaaggtc
      421 gctccagttg aagctgcgcc cgtcgggata gcgcgtcgtc gtcaccagcc ccgccaaact
      481 gtgcactttt ggcacatcga ccccatgcgc gagaaaaaac aggatgctgc gcaccgaacg
      541 caagcgcagc ggcaccacgt cttttccggt acgacggatg tcttgggtca gtgtgtagcg
      601 caccccgtct ttgctaagcc caagcagctt gcgcagtcga tcgagcggtt cggagtgatc
      661 ggacagacca cggacttcga gaatcagcga cggctgctcc ttggcgtttt cgtatgacaa
      721 gttgagcaag tcggcttgct gaagcatgcg cagatcggtc gcaaacttct gaaaatcggc
      781 ataggtcggg gcctgcatcg gcgttggacc ggctgccgac ggcgcgttgc gcagcgagcc
      841 cacccggtcc agacacagcg tcagcacccg cgcaatgctc cagcccgatc gcagcaagtg
      901 caaaagcgct tcctgcgaca gcggcgaaat cagcttgcgg acaaacgctt cgccggccaa
      961 aggggacagc accaccgtcg gtgtttgact gtatgtcatg ccgacgctgg gccgaatcac
     1021 gctgcgcagc ggcgcatcgg cactgacgga aaaatcggcg ccaatccctg ccgaaccacc
     1081 aatcgactgc tgggatgtga tgccgctgac ctcgacgaag tacggattgt cgcgatagcg
     1141 cagccgcacc aagttgagca aaagctgctc attttgagcc cgcacgatcg cttcgttgaa
     1201 gtcgcgctgg gtcactccga gcgtcgctgg ccccagctga aaacagcccc cgctaccaac
     1261 cagcagcagg agcgcaaaga aagcgcgcca aaccatgatg ccccttggtt aatctctcat
     1321 aaccaagcca tgagcgttaa gagccagacc ccagccgaag tttcccgtcg aatccgttcg
     1381 caagagctgt tcgcgcgggc ccaaaagacc attcccggcg gcgtcaattc gccggtgcgc
     1441 gcgtttcgcg gcgtcggtgg cgaccccgtg ttttttgcca aagcgaaggg cgcgtatctg
     1501 tgggatgtcg acggaaattc gtatgtcgat tacgtcggtt cgtggggccc gatgatcctg
     1561 ggtcatgccc agccacaagt gctggctgct atcacggcgg cggcggcgga cgggctcagc
     1621 tttggggcgc cgacagaacg cgaagtgcgt tttgccgagc ttctgaccca gcgcgtaccg
     1681 tcgatggaca aagtgcggct ggtgtcatcg ggaaccgaag cgacgatgtc cgcgctacgg
     1741 gtggcgcgcg gtgcgacggg ccgatcgaag attgtcaaaa tcgacggggc gtatcacggc
     1801 catgctgaca gtctgctggt cgccgcggga tcgggggcag cgacgctcgg tctgcccggt
     1861 tcggcggggg tcaccgaagg ggccgcgcgc gataccctga cagtgccgtg gaatgacgaa
     1921 gccgcgatcg aagcggtctt tcgcgatcac cgcagcgccg atggacagag cgaaatcgcc
     1981 gccgtcatca tcgaaccggt ctgcggcaac atgggctgcg tcgcgcccga gcccggctat
     2041 ctggaacatt tgcgcaagct cacagccgac gcgggcaccg tgctgatttt cgacgaagtg
     2101 atgacgggat tccgggtgtc gcacggtggc gcgcagaagc gttacggaat caccccggat
     2161 atgacctgcc ttgggaaaat cgttggtggc ggcctgcctg cgggggctta cggcggcaag
     2221 cgcgagatca tggacaaggt cgcacccgtg gggccggtgt accaagcggg cacactttcg
     2281 ggcaatccgc tcgcggttgc cgcaggcatg acgctgctgg agctgctcga cgcgccggga
     2341 atctacgaac agctggaaaa acagaccgcc cgtctgtgcc acgagctctt ggcgctgtgc
     2401 cagcaggccg gaattccggc ggtggttaac caggtcggtt cgatgttcac gctgtttttc
     2461 accgcgcagc cggtcaaaaa ctacgccgat gccaaacgat cggatactgc acggtttgcc
     2521 cgctttttcc acggcatgtt ggagcgcgga atttatctgc caccgtcgca gtttgaagcc
     2581 gcgtttgtgt cgtcttcaca cggcgatgaa gagatcgaaa aaacgattgc cgcggcgcgc
     2641 gaagtcatcg caacgctgta gcttttccgt cgacaaaatc gagcatccgg gccgaacagc
     2701 ggcccattga caccccgatg cgacgttgcc agattggcgc gcatccaaac cgcatagacg
     2761 aaatttcaag gagagaccgc ccatgctaaa cgcatccgaa aaagccccaa acttcaccct
     2821 cctcgacggc gcccgcaagg ctgtttcgct gtcggatttt gccggcaaga ccgttgtgct
     2881 cgcatttttc ccggcggcct ttaccggcgt ctgcaccaaa gaactttgca cgttccgtga
     2941 ctcgctcgcc gcgttcaacg acgtgggcgc cagcgtgctc ggaatctgcg tcgatgcgcc
     3001 gttttcgaac gccgcgtttg ccgaaaagaa cggcctcaac ttcccgatcc tgtcggacta
     3061 cgaccgcaaa gcggttcgcg cgttcggcat tgcgcacgat gacttcgccg gcatggcggg
     3121 ctacacggcg gcgaaacggt cggtgttcgt gatcgacggc agcggcacca tccgctattc
     3181 gtgggtcgcg ccaaaccccg gcgtcgagcc aaactacgac gaggtcaagg ccgccgtcgc
     3241 tgccgctcgc tagttcgtca gcttgtgaca cagcgaaaca tcggtcagtg tcacaatcac
     3301 ctgcgtcaga tcgcctttgt cgtcgtgctg cggaatgatg cagaccagca gccggcgagg
     3361 agccgcgtcg ctgtcttgcg atgcagtcac gatcacgtcg cggacggggc gcttcctggt
     3421 tgtcgcttca cgcagaagcg catcacagcc caaaagcggc accccggaag ggttggtcgc
     3481 gccacttctc gcatcgtcgt cagaacaccc ggccagcgac ccaacgcgcc cacccaacag
     3541 ctcgcgggcc gctgggttgc acagcgtgat ctgctggttt gccaaaagca caatcacacc
     3601 gacgtccagg ctctgcacca gcgcttgaaa gcgcccttcc gtcgtcgcct ctcgctcttc
     3661 cagttcccgc acccgcgccg ccaaagagac gatctgcgca tctcgatagt ccagatccgt
     3721 catggtcgac tcctggttct atcatccacc cattcgatcg tcttggatac gcgcgtggag
     3781 cgctcgatga cacggcgggt ctagttcgac gagcgttcaa acggttcgcc gaggatctgc
     3841 tcacacagct tgaccacacg ctggtgttgg ggaacccccc gcagcaaaaa ctcgcgctgg
     3901 cgctgggtcc gcgctgtttc caagcgatgt cgcagcagcc tcacggctcg acgcagcgcc
     3961 acccgtcctt gttcgtcgtg accaagtgcc agctcgacct cggccttgga cagccacatc
     4021 gacgcagcat acggcccatg atcgtccatc agcgccagca ccgacaggcc cgcttcgacg
     4081 gtcacagtcg cttcggaaat ccgcccaagc cgcaaaagcg ccgtcgtcaa gcaggaatag
     4141 ggatacacca ccaccgtcgg caaagctccc agcacatcga tcgcttggcg aaagtgctgc
     4201 accgcctctt cccactcgcc gcagtacaga tagttggcgg ccaaaatcga aatcgcttcg
     4261 ccctgacgga cgcgatcctc gccgcttgaa tcgaccacat ccaacagcat ctgccgctgc
     4321 gctcgccgta ccgtttcgtc gccgagcatc aagtcaccag cgccaatcat catctctgcc
     4381 gatttcagca ggtgcgaatc acccagcgtc gtcgcgtatt tttgcatctg ccgaccgagc
     4441 cgctgcgtct cctcccagtc gccaagcggc atgctgttaa gcgccgcatc gaacgtcacc
     4501 agcgacgcaa gccgaacctg tccaaagcgc aaataggtgt cagcggattc ggccaaaagc
     4561 gcttgggtcc ggaacatgtc gtcgtccaga tacagatcca tcgccgcctg cgcgcgctgc
     4621 cacgccgcga cgtacagcgg ctgctgggga tcgctttcgt acaagtcgca gaccgcgcgc
     4681 atctgcgaaa tggtccgcag cgccggctct cgcatcgcca aaaaagcata gagctgggcg
     4741 acgagcgaag ccgcttcctg aaagtaccaa gcggcgtccg gctggggcag tttgccgtcg
     4801 aaatcacgca gaacttccat cgtgacttcc atctttccag ccagcgttgc cgtcgccaaa
     4861 agccccaaaa tcgaccagta atagaagcgg ctaccgggcg gcgacagcaa gatgccttcg
     4921 cgggccgctt gcagggaatc ggacagtcgc ccggcaaaca gcgccatctg gccatccaaa
     4981 cagcgcaccg caccaagcca ttcaccttgc gccccgacgc gccgcgccgc caagcacagc
     5041 tcctgggttc gttccgcgcc gccccgcttc cagctatcaa tcgccgcttc gacatagtga
     5101 cgaacggcgc agggaccgtc gctgcccagc tcgaaatggc gcgccagcac ggcgtgatct
     5161 ggttcgccac tgcgctgcaa atattcggcg caaagtcgat gtcccagtgt gcgatcctcg
     5221 tcggtgagca gcgaataggc cgcttcccgc atcagcgagt gacgaatccc gaactcgaca
     5281 tcatcgggca agcgactgtc gcgacggcgt tcgatgattt ctgcgtcgga cagctcacgc
     5341 agcacttggt cgatgtgatc gccggtgcca ctgccgctgc catgtccgag cagcgccatc
     5401 acgccgccgc ggaaaaagac atcgccaaag atgctggctg cgcgcaaaac ccgccgctgt
     5461 tcgggaggca gacggcccac gcgggcttgc agcatggcca gcacggtgtc gggcaagtgt
     5521 tcggtttttc cttccgcaaa cgcgcgaatc agctcttcca aatacagcgc gttgcccgcc
     5581 gcttgctcaa cgatacgcgc caccgagctg gccggtgctt tcgcgcccaa aatgttctgc
     5641 accagccgct cgctcgcacg cttgcccagc ccacccaagt aaaactcttg cagaccgcga
     5701 tccttgccga gcttgggcag ccggctctcg tattcgggtc gcgccagcac cagcacaaac
     5761 agcggcgaat caggcagcga ccgcagggcc gactcggtga gctcgatgct ctgcggatcg
     5821 ccccactgca tgtcttcaaa cacgagcacg acgggctgat cggcgcagcg gccacgcaga
     5881 aagtccgtta ccgccagcgt cgcttgttcc aaaaacagcc gaccgtccga acgcgccgcg
     5941 tgcagcggtg gatacttgtc gagcggcggt gacatcccgg caagctcagc caaaaaggcc
     6001 gccacccgcg cggcctctcg ttcccccacc gcgtgagtcg ccagcgcgag catctggcca
     6061 aaaccgtccg gcgcgccttg tccccgaact tccgcgcaca gtccgcgcag caccgctgac
     6121 cacagaccaa acggcgcccc cggattcatt gcatcgccgc gcccaagcag cacctgaacc
     6181 ggcgtcgcgc ccgcacttgt gcgccgcaga aactcgtgcc gcagccggga tttgcccacg
     6241 cccgccggcg ccaccaccag cgccacccgc ggcgtcgact cttcaatcac cgtgcccagc
     6301 accgcgccaa gcgccaacag ctcctgttcg cgacccacac acggcgtcgg cttgccaagc
     6361 agcttgcgcg actcgtcgat cgagagccgc tcacccagca gggcaaatgc accgtgggac
     6421 agccgcacca cttcaaaccg cgcgtcgagc agccccgccg tcagctcatc aatcagcacc
     6481 gcatgcgtac cgccagccgt cgtgcgtctt cgcaacagca ccgccgcgcg atccacagct
     6541 tctccaacgg ggagatcacc ggccagcatg cctttccccg ttgcgatcgc caccaccgct
     6601 tcgctccagc gttcgtggat gtgcagtgcg gcccgcgccg cccgtcccgc caagtccgtc
     6661 gctgctcctg atccaagctg atgcatcgtc cccagcagcg atccatcgac cagttgttcc
     6721 agatgaagtc ccgacggcgc gaggtgtgcc cgcagcgctt ccagtcgggc cttttgcgcc
     6781 gcatcatcgg cttctgccag cgtctgctgc gacgacactt ccagcacatc gctgaccaaa
     6841 agcacactga ccagctgctg ctcgctgccg cggatcgacg ggccgaccac cgcatgttca
     6901 aacgcgggac cttgcagcgt tggcagcgtc ggtagcaaca gcagtgcggc cagcaccgcg
     6961 ccaccgtccg cgggccgcag cttgggatct ttgtggagca tgcgcagcag caaatcttca
     7021 agtggcagcg gcagctcagg ccgcaccgtc cgcagcttgg gcgcgtcttc aaacaggatt
     7081 tttgccagca ccgcagcgac gtgggctgcg acaaacggcg gactgccggc aagacactcg
     7141 taaaacacgc agccgagcga aaacacatcg gcggctgccg acagctgcga ctgtccgcgc
     7201 gcttgctcgg gcgccatgta ttcgggcgtc ccaatgatcg ccccagtgcg ggtcatcgcc
     7261 gccgaacgaa cccccagacc ccgtcgcgca atcccgaaat ccagcagcac caccttgccc
     7321 ggatctccgt cgaccaaaaa caagttcgac ggcttgatat cacggtggat gatgccccgt
     7381 ttgtgggcga tggatagggc ttcggcgacg cgctgaatca gcagcagcga ctcttttagg
     7441 ttgagcccgc cgtgtcgcag ccgttccgcc aggtcgattc ccgacagcca ctgcattgcc
     7501 aaaaacggat gccctgcatc ggtgcgccca tgcgcgacgt agccaacgat gcctggatgg
     7561 cgcagctccg ccaaaagcag cgcctcgcga tggaaccgcg cttcatctgc cccatcgaac
     7621 agcaccttga tagccaccgg ctgcatcgtg gtgcggtcca ttgcgcgata gaccgcgccc
     7681 atcccgccac tgcccgtcag ccgctcgacc tcgaaccgcc catggaccaa gctgcccggc
     7741 gctagctctt gtagcatgct gcgattttag ggtcgggacc gcttggactc taggcggttt
     7801 gtgcgcgcag acgaacagcg gtgtggcttc gtcggacaga gccgttacga aatctgcgca
     7861 atcagcagcg tgatgtcgtc tttgcgtggc ttgtcggcat aaaactgcgc cgcctgcgcc
     7921 atcagggcat cgcgcatcgc cgccggagcc agctcgcccg tctgaccaat cacctgacgc
     7981 agccgctttt caccaaactc ttcgttcatc gggttttcgc actcgatcag gccgtcggtg
     8041 aaaaacacga tcttgtcgcc cggacgaagc tgctggttgt tttcggcaaa cgtcgattcg
     8101 ggcacgtcgc cgagccggtt gcctcgcgac atcagcacgg taaacggcga ctccccttcc
     8161 ttgagcgagc tgggctgccg atacaggtac gggaagttgt ggccggcgtt ggcgtaggtg
     8221 atgccgcgcg tcttttggtc gatgacacag gcaaagcagg tcatgacaaa cttgcgacgg
     8281 gcgctctcgt agatcgcgtg gttcatgatc tcgaggatgc gcgacgaggt gatccgctcg
     8341 ccttcgaggg cgcgaccgac atcgcaggcc gctttggccg ctgcagtgat catcgccgct
     8401 ggcacgccgt gtccggtcac atcgccgatg atgagcagca cgcgatcgcc cggtagatcg
     8461 tagaccgccc accagtcgcc tccgcactgc gtcgctggct ggaagtgacc gacgacttga
     8521 atcggaccgc gctggatgac ttccgacgtc ggaaccagcg tttcctgaat cgttcgcgcc
     8581 agctccagtt ctttttcgag cacggctttg gccgccgttt cttcgaggag aacttgcagc
     8641 cggtcggcca tgaagttgaa gttttcggcc agaagtccga tctcgtccgg cgactgaatc
     8701 tcgacgcgag tggtcagatc accgcgcgcg atctgatcgg cacgctgcgc aagctgctgg
     8761 atcggtcggg tgatccgcaa gctctggaaa atcgaaatgc ccgccgcaaa cgccaaaaag
     8821 accagcccga gcacagcgac ctggacgacg aacgcctgac cctgcgccag cttgtattcc
     8881 tcgatcgagc gcagctggtt tttcagcgat gccatgctgt agatcacaac cagcgtgcca
     8941 aactttttgc cctgctcggt ggcgacggga cgggcaaaca cgcggcgggt gtctttctga
     9001 ccgccctcgt cgacatcaat cgtcttttcg agcggcttgg tcagtttttc cagttcgggc
     9061 ccgagcaccg gatcgacttc ggtgattttc ttttccgcgt cgttccattt ttcgtcgctg
     9121 tgcgccgcaa catagccagt gtcactgatg acatacagcg ccttgacgtc gggatcggtc
     9181 ttcgcggcct tgggcacaaa cgtcttcaga ctgctgtagt ccgactgcat cagcgccgca
     9241 cgcgcactct ctgacatcgc agcggtcaag gtctgcgccc tggctccgag tgcagagtcg
     9301 taggactccg atagcttctg ggctgcctca tcgaaggccc gtcgaccgta gtaggtacta
     9361 gaggtcccga gcaggcccgt aatcagaatg atcagcacca ggctggtgga gatcatcttt
     9421 acgctaatcg acacaccgcg ctggctcgac attatcccga gtctacgcac ccttgccccg
     9481 atggtcaatc ctcgctacga tcgcgcgtat gcatccagcc ttggagcggt tcatccagca
     9541 gtttaaggca acgtcggacg tggtcatcga cagctggttc atctgcgaca gtgaacgcaa
     9601 catcgtcgac tacaaccgcg cctttttctc gatgtttccg cgtccggaag cacgtcagct
     9661 caaggtcaaa aagtgtctcg atgtgatgcg gctgacgatc tgccaagatc gctgcattgc
     9721 gcagcagtgc tggcgggaag gtcggcaggt tcgtctcgac gagatcgacg gcaagccggt
     9781 gggtcagcca gacgatgcac cgccgcgtcg ttacgtgctg tcggcgattc cgatcctcga
     9841 cgaaaacaac aagccagtcg gtgcgctgga aatacagcgc gatgtcaccg acgaagcgct
     9901 cgtgcaagac aagtacaaaa aaatgctgga aaatgaagca cgcgagcgcg agcgtttggc
     9961 gacgcagatc cgttcgcgca cccgtgagct atacgaggcc aaccagcttc tgctcaaagc
    10021 gcaggccgag cttacgcagc agaaaaaagg cctggcattt taatccgtcg agcgactgtg
    10081 ggggacaagt cgttcactga agccgtctgc gggctggggc tcggcgacga aggcaaaggc
    10141 agtttcgtcg atgcctgggt cggacggatg cagacgccgg cgctggttgt tcgctataat
    10201 ggcggaccgc aagcggctca ccacgtcgtt cttcccgacg gaagatggca ctgctttgcg
    10261 cagcttggcg ccggcatgct gcaaaaccag acggaaacgc tgctgtctcc gtacatgctc
    10321 gtcgaaccgc tatcgcttgt gcgcgaagcg acccacctgg ccgagttggg tgttgggacg
    10381 ccgctcttgc gaacgtgggt ggcccgtcgc tgtgtggtgg tgacgccgtt tcacaagctg
    10441 cttgggcggc tgcgcgaagt ggcccgcgga aacgcacgac atggcagctg tggacttggg
    10501 gtttcgcagg cgtggctcga cagtcaaaag ccaggtcttc cgtcgctgcg gatcgatgac
    10561 ctgctcgatc cggggacgct ctgttccaag cttcgctttt tgcaactggt caaagtcgat
    10621 caggccgaac agctcctcga cagcttgccg ccgcagcagc gaaccgcgga cgcgcaaaga
    10681 ctgctcaccg agcttcagca tcgcgatgtg ccgcaaaagc tggccgatga ctacgcgcgc
    10741 tttgtttcgt cgggcgtgca ctttgtcgat gaccagcagg ttcaatcgat gatcgcaaag
    10801 ccgcagcggc cctgcatttt ggaaggtgcc caaggcgtcc ttctcgacgc cgagcacggt
    10861 ttttttccgt tcgtcacgcc aagcaaaacc agtctgtttc aggccaacct cttgctaaac
    10921 ggacgcccga gccgccaagt cggcattgtc cgggcatatg cgacgcgaca cggaccggga
    10981 ccgctggtca gcgaagacgc tgagcttacg cgggtacttc gcgaaaccca caacgtcgaa
    11041 aatccctggc aagggccgat gcgcgtcggc tggttcgatg ctgtggcgac gcggtacgcg
    11101 ctggcggtcg tatcggggca ggtgcacatc gcgctgactt gcctcgatcg cttggtcggg
    11161 ctttcgtcgc tgtatctgtg caatcgctat cgctatgacg gaccgtgggc agcggacttg
    11221 gatgattttt ttgtcttcga tgcagccaac cacgtaagcg atctacgacg cgccgcgcag
    11281 ccgacccttg cgcgccagca acggctcact gagcttttgg cgcactgtcg accgattttg
    11341 caaacgctgg cgccgattcc gacgctgtta caggcaaagg gctcgctcag cacggcggcg
    11401 gatgcgtata tcgacgcgct gcttgcggcc atcaagctgg atcgaaacgc gctgatttgt
    11461 ctgtccgtcg gggacaaagc gagcgacaag cgctggttcg actagcggtc tttggcatca
    11521 gcgacgggta tcgtcactgc ccaggatgcg ctgcgccgct ttccagccgc tcatcatggc
    11581 gccagcaatg ccgtgaccgg ctcgcgtcga tgcgccgcac agaaacagtc cgtcaatcgg
    11641 ggaacggtag ccgggccgct tgtcgagaaa ctgatccggc gtggccgcca aaccataacc
    11701 cgacccgtcg ctggcgcgcg taaatcggct gtgactcagc ggcgtggcag actcgcgata
    11761 acaaatccgc gctgccgtcc ccggaaacag cttgtcgagt cgcccaatca ggttgtcttc
    11821 gatttgcagc ttcaggtctt ggtatttttc gcttttgcga tagtcccagc ccaacacgtc
    11881 cgcgtcggaa atgccccacc gtgacggcgc ccccggcaca atcgtcatca cttcgacgtt
    11941 ggtcaccccg acgggcgcat ggtgcagcca gtgttcggga tctttgacac tcgcgcttgt
    12001 gatgtacgcc gcttggacct ctggcagctc ggtcttgtgc gtcgccgaaa gcttgtacat
    12061 cgcgtcgacg tcgtagtggt cgtactgcca ataattggtc gcgcgcatcc cgtcctgtcg
    12121 cagatcgccc tttacaccca ggaacgtcat aaacagcgcc gctgccatct tcgactcacg
    12181 cgcccgcgtc agccactcgc tcggcagatg ttcgggcccg accagatcga tcagcgtccg
    12241 cttgaagtcg gcattcgaca aaacgacatc ggcgcgtacc tgccgcaccg gatcttcatc
    12301 gcgcgcggcg atctgcacac cgacggcccg accgtcttcg atgagaattt tttcaatcgt
    12361 gtggcgcaga cagatgcgtc ccccgagaga ctcgatccgc gccgccagcc gatcggcaat
    12421 gacctgaccg ccgccgcttg ggtaaaatgc gccgcgaaaa taatgcgcac tcaggcccaa
    12481 gtgcatcatc gcgctgactt ggctgggcgg cagtccatag tcaccgtttt gcccgagcag
    12541 cacggcacgc agcttgggat cgctcaccga aatggcatcg aacatctcgc tcgcggtcga
    12601 ccgccgatgg ccttgcagac gcagcgcatc gacggccagt ggcagcagcg tgcgcagctt
    12661 cgggcgatcg gaaaattcga tcgcgcgcat cgctttgccc accgacgaaa gcagcttgac
    12721 atagcgttca atcggcacgc gctgcgacgg aaacagctcg cacagccgct tttgatacag
    12781 gtccaggctc gccggtatgc ggaaccgaaa gtcgggataa atcagcgtgt cgaagccatc
    12841 ttgttcgagc ggcacatagt cgacgcgcac cgaacactcg tcgagaatgc gcggaatcgt
    12901 gccctgccta tcacagtcac cgatgtagtg cagtccgacg tcaaagtgat agcgcgcgtg
    12961 cttgggtccg cgacgaaact gcgtcgcaca gccaccggcc acgtagtgcg attcgaacag
    13021 cgcgactttt ttacccgcct gcgcaagcag cgccgcagcg acgagcccac cgggtccgct
    13081 gccgatcacc gcaacgtcta ctttcgccgg cagatgggaa tgcacccgtc gccaggcagt
    13141 cgtcgaaacg gggggtcttt gtggctcgtc gtcgcgcatg ttggtgtttg actctgcgtt
    13201 gtccggttaa ccgagcggtg ccagaagctc gcggtatttt ggcagtggcc acagccgcgc
    13261 atcgcactga tcttccagcc cgtcgagcga ctcgcgcagc acttggcacg ccggcagcac
    13321 atcccgcgca aacgccgccg tccgcttgtg cagatcgtcg gtggcatcgg cttcggccat
    13381 cgtcgctttg agcgccagca gtcgctgata cgcctgttcg agcagctcgg cttccacctt
    13441 gatcgccgca cgcaccggat cgggcgtggt gatccccgcg gcttgcagct gggtcagaca
    13501 cgccgccttt tgcttcacgt cttcgaggat gaccggcagg atctgcgttt cggccatctc
    13561 acgcagcacc tgggcttcaa tttgcaggct cttgacgtac tgctcgtgtc gaacatggct
    13621 gcgcgcttct tgttccggct tggacatgac gccagcacgc tcaaacagat cctgtacttt
    13681 gctgtcttcc cacgccgata gcgcttcgac ggtgctgcgg gcgtgtggca gaccccgacg
    13741 ggccgcttca tcacgccact gcagggaata gccgtttccg tcgaaacgaa ccgcgcgcgt
    13801 cgtagccacg acctcgcgga tcacttccat gatcgcttgc tgacgatcac cggggaactt
    13861 cggctcgact tcgcgcaccc gctggttcat ccatgaaagc gcgtcgccga tgatggtctg
    13921 aatcaccgaa agaggcaccg caatcgcctg gcccgctcca acggcgcgga actcaaactt
    13981 gttgccggta aatgccatcg gacttgtgcg gttgcgatcg gtgtagtcct tcgccagcac
    14041 gggcagcgcc ggaatcccca gatcaagctg cttgcgttcc gacgaagaaa ccaccgcgcc
    14101 gctctccact tgatcgagaa tgcggttgag ctgctcgccg aggaacaccg acataatcgc
    14161 cggcggggct tcgtttgcgc ccaagcggtg atcattgctc gccgacgcca ccgatgcccg
    14221 aagcacggcc ccgtgacgcc acaccccaag cagcaccgac gacagaaagt acagaaactg
    14281 gagattttgc tccggcgagt gacccggatc gaacaggttc tgaccgtcgt cgctaccgag
    14341 actgacgttg acgtgcttgc ccgacccgtt gaccccggca aacggctttt cgccgagcag
    14401 caccgccaga tcgtgccgtt caccgacttt tttcatcact tccatcagaa gctggttgtg
    14461 atcggctgcg acgttggcgg gctcgtaaat cggtgccagc tcaaactggt tcggggcgac
    14521 ttcgttgtgg cgcgtcttcg ctggaatccc gagccggaac agctccagct ccaagtcctg
    14581 cataaagcgc agcacgcgct ctttgatcgc gccaaagtag tggtcttcca gttcctgacc
    14641 tttgggtggc cgggcgcctt gcagcgtgcg gttacaaaac atcaggtcgg gacgcgcctt
    14701 gtacagctcc aaatcgacca agaaatattc ctgctcggcc ccgcactgcg ccgtcaccac
    14761 cgcgccatct tggccaaaca gcgacagcgc ctgcgccgcc tgctgtccga cgaaatccat
    14821 cgcgcgcaaa agcggcactt tgtgatcgag cgcttcgccg tggtagccga taaacgccgt
    14881 cggaatgcac agcgtcttgc cgagcggccc cgccatcagg aacgccgggc ttaccgggtc
    14941 ccacgccgta tacccgcgcg cttcgaaggt tgtacgcaga ccgcccgacg ggaagctgga
    15001 ggcatcgggt tccccttgga taagctgcgc gccggaaaac gattcaatca gtccaccgtg
    15061 atcgtcccag gtgatgaatg cgtcgtgctt ttcagccgtc gcgccggtca tcggctgaaa
    15121 ccagtgggta aagtgggttg cgccgcgctc aatcgcccag tctttcatcg cttgggcgac
    15181 gacgtgagcc acttcggcgg acaggcgctc gcctttgcga atgcacttgc gcagcgaacg
    15241 aaatacttct ttcggcagcc gttcttgcat ggtgcgccag ccgaacgtgt cgcagccgaa
    15301 gtacgaggag actttgagtt gatcgagacg ggagatgctc tcagagcggc caatgcttga
    15361 catgggcggc gactttagca cggcttccca tcggtggcag agcaaccgac atgtttgcgc
    15421 tggcattacc tatttctttt gtaaaaatgg tgtcttatga cacaaaaaac aaatccagca
    15481 ttgggcgcgc aacagctgtc gcgtcggggg tttctttata ccgtggtggc aacgacagcg
    15541 tcggcactgc tgttgccgca gaccggctgc gaaaccgcgc caccgcagat gatgatggcc
    15601 gatggaaagc acttctttcc ccagtcggtc gcgtcgggtg acccgcgcgc agacagcgtg
    15661 gtgatttggg cccgtgcgct caatccggcg tcgccggctg agccctgcca agtcaaacta
    15721 caagtcgctg ccgatacgtc gtttttgacg gtggtgtacg acgggatgcc gatcaacttg
    15781 acgacggacc aggaccacac ggtacgaatc aaagtgacgg ggctgtcggc acgcacgacg
    15841 tatttctatc ggtttttggt gctgcgcgac gatgtgaccc tgacttcgca agtcggacgg
    15901 acccggaccg cgccaagcgc cgacgacaag gtgccggtca agttcgcctt ttggtcgtgt
    15961 caggacttcg agggacgcta ctacaacgct tggcagcggc tgctttcgct cgacgaggat
    16021 ctcgattttg tgctcgggct cggcgactac atctatgaaa ccgcgccagc aaccccgacg
    16081 agcacgccgc gcagcgtcgg tttttccgat cccagcggcg ccattttggt cggcacaagt
    16141 cggatcgctg cgtcgacggt gggtcagtat cgcgatctct acaagcgcta tcgttccgac
    16201 gcggtgctgc aaaaaatcca cgaacgctac ccgatgatcg tgatctggga cgaccacgaa
    16261 tactcgaatg actgctgggg cgcgacgagc acgtatcaaa acggcaagac atcagaggtc
    16321 aatggaaccc gccgtcgcaa cgccgagcga gctttttttg aatacatgcc gatcgacgac
    16381 agctcgccga tgtcagggca gctcaccgtc gctgattcaa gcctttaccc acgcacccgg
    16441 ctgttccgcg acttccactt tgggcaaaac cttcacttgg tactgacgga ctatcgcagc
    16501 ttccgtcccg atcacttgat tcccgaagag gcatatccgg gcagcgcagc gctcgatgcg
    16561 tcggccctgc gggcgcttgg ggcctatccg attttcgagt tcgatgatgg ctttgcggcc
    16621 atcgacatcg atgcgccgct ctacgcccgg caaaagcagg tgctgaccca agccgcaatc
    16681 gccggctgtc tggcggctgg acttgcgcag gccgatgccc aacaaaaagc ggcgtcgtgg
    16741 gtgacaggaa atttgtcact tctgtatgtg aacgccgtgc tcaaggccac aaaccagacg
    16801 gccttgatcg tcgctccgtc gcgagacatg gatcgcgctg tgtcgtggct gcacctgggc
    16861 aagcagcagc tcttttcacc gcaggggtcg cgctatctgg cggtcaagcc gctgttcgag
    16921 ctgtatgcgc agtatcagtg gcagctgagc cagcacgaga gcgagaacgt ctttggcaaa
    16981 gaccagcaag agtggctgca aaaaacgctg accgaatcga agaccaaatt ccgcgtgatt
    17041 ggcagctcgg tgatgccgac ggaggggatc ttcaacctga ccaccacgcc gggtctgccc
    17101 gccgcgtatc agaatgtctt tgtctacggt ctcgacggct gggacggctt cccgaacaag
    17161 cgccaagagc ttctcgactt cctgaagaca aacaacgtcg gaaatacgtt cttcgtcgct
    17221 ggagacatcc acggcggctt tgtgtcggtt ctggatggca cggcgccagc gctcaccacc
    17281 ccggcgataa gctcgggaac gctgcaagag tcgatcgccg aatctgcgct cgcgctcggc
    17341 ttcaccctcg attcacccgc ttatgcgaaa gtcgtggcca acctcgacaa gacgcttcaa
    17401 gaaggcaacc catcgatcgc tttttcggca agtgatcagc acggcttcgt catcgtcgag
    17461 gtcggtgaaa ccgaagcgca ggcgacgttc cacctgataa gccaagccga ggtgaagacc
    17521 gattcgacca gcctgcccgc cgccgccctg gcaatgaagt ttgcgacgca gaaattcaag
    17581 ctgtccggcg cgaccgtcac caagctgtag cactcgcccc ccgacgagcg actactcttc
    17641 ttcttcttcg acgtgcttct tcgccgacgc aatcaccttg tcggcgagct gctgcggcat
    17701 ttccgcgtag tgcgaagtcg ccaggctaaa gctgccgcgt ccacccgtcg ctgagcgcag
    17761 gaacgccgag taatcgagcg tctcactcat cgggatctgc gcgatcacga tctggtattt
    17821 gcccttggct tccatgccag cgatgtgggc acgacggttg ttgagatcac cgatgatcga
    17881 ccccatgtat tcctcgggaa tcgacacttc gatcttcatg tacggttcga gcagcaccgg
    17941 cgaagcttgc ttgaatgcgg caaggaagcc tttgctgccc gccatttcaa agctgcgcga
    18001 gtcgctgtcg acgtcgtggt attttccgtc gaacagacgc actttcacgt cggtgatcgg
    18061 gtagccggcc agaccgccac ggaccatgcg ctctttgatg cctttttcga ccgatggaat
    18121 gaactgtcgc ggaatcgatc caccgacgat cgcatcctcg aacacgagac cggtgccacg
    18181 cggcgccggg tccagatcca ggtagcagac accgaactgg cctttgccac ccgtctgttt
    18241 tttgtgtttg ccttcgacgt ttttgacccg accgcggatc gtttccagat agggaactcg
    18301 cggcggtgcc agctcgactt ccacaccgac gcggcgcagc ttgtcgagcg tcgcttcgat
    18361 gtgcaccgga ccacagccgc cgatcagcgt ttctttggtg gccggatcgt attccaccga
    18421 cagcgacaga tcctcttcgt ggatgtcgtg cagcttctgc gccagcttgg cttcgtcggt
    18481 cttgcccttt aggtgcagcg aatagcgcac aaccggcgcc ggtaccgtcg gagccgcaaa
    18541 cttgacgagt tctttttcat ccgaaaacgt gtcgccgatg cgggtcagct tcagcttggc
    18601 cagaccaaca atgtcgccgg cgtgggcttc cggaacgctg gtccgcttgc cgggcgtgaa
    18661 cacatagagc tgaccgacgc gctcgccttt gtcgtggctc gcgttccaaa tcggcgagtc
    18721 cgccgcaatt ttgcccgaga cgatccgcac gagcgccatc ttgccgactt ccgtcgggat
    18781 gatcttgaaa acatacgccg acagcggctc gctgctcttt ggcgagcgac ggacttcgct
    18841 gccgctcttg ccgctgccgg tgcgcgtccg tcccgccgaa gccggaggaa accacgccac
    18901 catcgcatcg agaagcggct gcacgccacg gttctgcgcc gccgatccac agaacaccgg
    18961 aaacagcttg cccgcggcga ctgcgcgctg aagtccaagc tgaagttctt cgtcggtgag
    19021 cgtaccatga tcgaagtacg ctgccatcag atcatcgtcc gatccggcga cctcttcgat
    19081 cagcttgtcg cgtgcttcct tcgtcgctgc cgccaagctt gccggcacat ccgaaaccgt
    19141 cacatcacga ccgtcttcgg aaaaacgata ggccttctgc gcgaccagat cgatcatgcc
    19201 ctcgaacgag ctttccgatc cgatcggcag ctgcaccgcc accgcttgcg gcgacagcac
    19261 gcgatgaatt tgatcgagca cgacggcaaa atcggcccgc tcacgctcca tcttgtttac
    19321 aaaaatcatc cgcggcatgt gcagcgcgtc ggcttcttcc caggtctttt cggtgtagac
    19381 ctgcacgccg tccgacgccg acaccacaca aagcgccgaa tccgcagccc caatcgcaat
    19441 catcgtatcg acgacaaaat cgccctgccc cgacgtatcg atcaggttga gcttgccttt
    19501 ttgccactcg accgccgtga cgtgcggatt gatggtgcct ttgcgctttt tctcctcggg
    19561 ctccgaatcc aggttcgagg tctcatcatc aacccgaccc agccgcgtca ccagcttggc
    19621 gtcaaagaga atggcctcac ccagcgaagt cttcccagaa ccaccatgtc caatgacgac
    19681 gacgtctcga atctctgctt cgctgtagtt gcgcattgct tctgtctcct aaccagtgct
    19741 ttcccgtctg tgaacaagcg tgccgacgac gctgccagcg cttgcttgtc cttagggccg
    19801 gggattttac cttctcaatc cgcgcgcggt agggcttttt ttgacgtact gctccatgtt
    19861 gtggaccgct tggcgaggtt tgccgctctg catctgggtc cgcgccaggt agtagtacac
    19921 gccgggatgc cgctttacat actcttcgtc gtcggaaagc tcggccaaat ggtccagcgc
    19981 ttcgggcgac aagtcggctt tgtaaagcaa atatccgagc aaaagccgca cgctcggacc
    20041 catctgatca atccacttca tgggccagtc gcgcagctcg cgcagcgccg gcacaaactc
    20101 gcggcgattg aggtagcgct tggcatcgct ccactgtcgc aaaatcgtcg cctgttcggt
    20161 ctgcgtaaga cccactggca gcgttggcgt gcccaaatca tcggcaaaat gttcgtcgct
    20221 gccatcccaa ttttcgctca gcgcttcttc atgttccgtc gctgcgcgct gcccaaacaa
    20281 ggtaagcagc tgcgaaagtt cgtcggtctg cgccccaccg ccaatccctt ccgatcgatt
    20341 gagcagtcgc gccgaagctt tgcgcttgcc ccgcatcaac agtgcgcgtc cgagcgccgc
    20401 gtacagatcg gcccgccgaa cgccgctgcc gtcgtcaggg agccacgccg gcggcttatc
    20461 aaatggcatt ccgtgtccga tgagatagcg ctcgaaccga ccataaatcc agtcgtcgct
    20521 atgcacccgt ggcaccgatg attcgtgccg catataaccg agcagatcgc gcggcgcggc
    20581 aaattcgatt ttcgcgttgt cgtcggtgtt tatgtgtccg ccaactgaaa acgcccagat
    20641 ttcatcgggg gtcaaaagca gatcagcgag cacttcttcg ggccggtcga tccctgcgcg
    20701 tgacagctcg gcgcgcagcg acggcacctg gaaatttcgc cgcagccggt tcagatcaag
    20761 cgacatcggc tgcttggttg cgaccaagat catgtcgctc gatccgtatt ccgacgtaaa
    20821 gacgtaggtg tacggaaaca cggtcgcaaa gctgcgcagc agtgtcttga tgttcaccgg
    20881 cgaaagctca taaagctgcg cccactgaca gaacacaccg tcgtcggcca gccggctttt
    20941 ggccagcttc cagtagtcaa tcgtaaacag gtttgaaacg ccggtgatcc atggattcga
    21001 cggctcactg acgatcacgt catagcgatc gtcgggcccc tgcgcctggg tcatgaagtt
    21061 ccgaccgtcg ccgatgatga cgcggacgcg cggatctttg ctgggcaggt ggctgacttc
    21121 ggcaaagtaa cgatcgccgg cttcgacgac cgcgggttcc agctcgacga catcggcgtg
    21181 tccgatcgga aactgtgtca ccgcaccgat cgtcacccca gatccatatc cgacgaccgc
    21241 aacccgcggc ggtttgtcga gcgccgtctt gtgccaaagc agcggcagaa gcccgaccat
    21301 gatctgcgtc cccatgtcgt cggaattcga ggcgtcgact ttgccgttgt ttttcagcgc
    21361 aatcgtcttg ccccagcgct cgaccgacac cgtcgtcgcg atgccgtctt tgtaataaag
    21421 caagtcgggc aaaacgcggt tgtgcagccg caccaccgat cgcgccaccg aaacccgaaa
    21481 cagcccggcg gcaaagtgcg tcaggttcca gcgcggcgac aagaccgcaa acagcgctgc
    21541 cgcaagcatc acgcaagccg ccgtcaggta aagcctgcgt cgcgaagcgt tcggcacctg
    21601 tggcgacgga tcaaatgcat ccaaacctgt ggctttgccc ggtttttggg tttctgcgac
    21661 caaaatcagc atcgccgcaa gtcccgccgt cacaagcgcg ctcccgagca gaccgcgctg
    21721 aagcccaagt cctggcagca ccaaaaaacc cgctgcgaac gacccgacga tcgcccccag
    21781 cgtgttgagc gcgtaggcaa tcccgacatc atggcccgcc cccgacgcgc tgccggtgta
    21841 aatccgcatc gtcagcggca tcacgccgcc catcgccagc gtcgccggca agatcgccag
    21901 cgccgacaga ccaaactgcg tcgataaaag ctcgtcgatc cccaggttcc ccgatcgcaa
    21961 gatcgccaaa aagatccccg gcagccggtc aaacatcaga tacgaatatc cgacggcaac
    22021 cagcgtcagc gcgtgcagcg atccgagcca cagcatggga cgcgcgatcc gcggcaagag
    22081 cctggtgatg atcgccgccc ccgccgccag cccgataaga aacgcgagca ggatcagcgt
    22141 gaacgaatac accgacgatc caatgatgat cgccagcgcc c
//
LOCUS       DAORGN010000069        22147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_938_length_22147_cov_5.326272, whole genome shotgun sequence.
ACCESSION   DAORGN010000069 DAORGN010000000
VERSION     DAORGN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22147
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_22147_cov_5.326272"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..3966
                     /locus_tag="PKL17_09695"
     CDS             28..3966
                     /locus_tag="PKL17_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNK45045.1"
                     /translation="MLNEARAAARLQHPNVVTIYRVGEVDDHPVIISEFVRGQNLDAL
                     PKPVPWTRAVEIGLGLARGLAAAHRRGVLHRDIKPGNVIISNDGEVKLLDFGLAEFFD
                     ADGQGADPEDLMWSQAAEMASVSGQYATVNMQVEDAARVMAVDSPSSNGSTSNPEDKE
                     FVVVVNRKHVDPSSSTPEQKVLRQGPPSRSPVSVRGKMPQAPAQRFPTIPPSDSEPQL
                     GPRSRVNPSASTPEAQPEGRTPVQLPAYRPTPRPSAPKHQSRIAGTPLYMAPEIWRGE
                     PVTRETDIYAMGALLYELCAGAPPHIEVPLTELPRIASEQDAPPLLRVAPTVDPRFAE
                     IVDRCLKRDPRRRFASGDDLREALEALRPREGKVSAPEGNPYRGLLAFEAEHRSLFFG
                     RTNEIGTIIDRLRTEAFVLVAADSGVGKSSLCRAGVLPLVTDGALGNGRTWSVLLMVP
                     GRNPLVTLCELLAPLMQTPGDKLMEKLRNEPTSLAWELRKKLGDKTGVLIFIDQLEEL
                     VTLADEHEVQTLGAALGRLCTRTPGIRMLATVRSDFLARVATVPSLGDELARALYILR
                     PMTSDKIREAIVGPAHAKGVRFETAAMVDQLTESTAKTDSGLPLLQFALTELWEARKG
                     DVITEDSLAKIGGVTGALARHADQVLMSLPADQRAAARRILMMLVTLEGTRARRTQEE
                     ILINPAAHAAAEALVKGRLIVARDTGEALAYEVAHEALIRGWDTLKRWLEEAAESRQV
                     KGRLELAASEWHRLGKTREALWSERQLAELVLLDDSDVTVKERAFIVASRGAAQRSRF
                     IRRALIIAVPAVLTVIWLGVRLESARNQRNKVDLYRDRGQSIFTEAKKRAAEAATLRQ
                     QAYAAFDAMDKDRGESLWASALEAGNDAEDLYTKSEYSYTAGLALDSTRADVRSGISD
                     VLYDMALLFEQTHQKQKLPDVFARLQLYDTEGSRISKLTEPAVLDIAVEPADTEIEIG
                     RYVLDKKQKYHLADIHRLGATPLMGQTLPPASYLLTFRKSGHEEVRYPILVSHGERLK
                     IDFRVPKAGTIPAGYVYIPPGRFLFGSAADEIIRAGTLSTVPLHTVQTQGYLISRTEV
                     TFGDFLHFAHTASKEERSKLPILSNEKNSVFLRESIPEQWALRVQHASEITETAQDGT
                     WLYKNRKTHSQADWKKLPLGGINAYDAQIFIDWLSSAGKLYGARFCTEHEWERAARGA
                     DSRDFPHGNRLDPHEANFDETYGQDGKSAGPDMVGTYPDSMSPFGIMDMSGNVFEWTH
                     SSLSKGQILVRSGAFWQPKFTANIANRSAMDSSFRDSMLGLRICISAPSN"
     gene            4284..5519
                     /locus_tag="PKL17_09700"
     CDS             4284..5519
                     /locus_tag="PKL17_09700"
                     /inference="COORDINATES: protein motif:HMM:NF039450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HNK45046.1"
                     /translation="MSLRTNTGIWTLALALLSGCPTPQQDGSVPDYRTQAVRTINGVG
                     LNGIGLNGIGLNGIGLNGIGLNGIGLNGIGLNGTGLNDSSLNGTGLNGTGLNGTGLNG
                     TGVTGLSLSSVTYNGTGLNGTGLNGTGLNGTGLNGIGLNGSELFIAVNAQTILRGATV
                     VGTEFHYKYQAPGSSTAAQFTVRIDAASIDPNAKIPSGQTNDVWIYKLVVRQDGMTPP
                     SEWSSLCYTEQGAPDTALLLSNRWDQTTGNRIDTPTFITFACRDFALGKCARMGYRPW
                     AKSSSCTGSGSNRKCSDVALVDHHQSCVRMMRADYCGNGKSYTVNGTLIDIYDYLNPP
                     IQVPETDWDIEARWTPTGAICINEPRHPELVGKWRRPDCNGDGKPDSFPACGQSDNRY
                     SGKGLVVDRFDDDQSGCGK"
     gene            complement(5538..7094)
                     /locus_tag="PKL17_09705"
     CDS             complement(5538..7094)
                     /locus_tag="PKL17_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNK45047.1"
                     /translation="MLLRALIAILLVSTGCRRQQIPADELVVLIEQPPRNIDPRYATT
                     SYDYKLAYLSYARLVSVDDDSLRPKLELAERVDVVSVSPEQGMTLEVTLRDARFSDGN
                     PVTADDIRYSIDKLSDEKTGSPSMRRRFAESGLIRPIEVLSPRTLRLRYAKPPASYLT
                     DLDFGIMRQGTAEPGQGMPIGAGPFVLDGEVGEVTRFARNRQYFGGVPAVAHLTVRVV
                     RDANSRLLSLVGGSADLTQNTVSPLLFDSVERWKDRLELTTAPSALLLYLGMNTEDAR
                     LRDPRVRRALALAIDRERIIRTKLRGKATLASSLLPPFHWAFHQPATSLAYNPDEAKR
                     LLDEAGFPDPDGPGGKPRLTLLYKTSTDALPVAIARVIAAQLAEIGVAVEIRPLEFHL
                     FLSDVKKGNFQLYTLSSAEIAEPSLFRNFFHSSYIPSAKNLDAGLNRMRYRNPVLDEL
                     LDQGSREIDRERRKEIYAKVQDLLAAELPMLPLWHPDNVTVARKVVNGFRMWPTAQLS
                     GLSSARKVGR"
     gene            7331..8953
                     /gene="dacB"
                     /locus_tag="PKL17_09710"
     CDS             7331..8953
                     /gene="dacB"
                     /locus_tag="PKL17_09710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNK45048.1"
                     /translation="MPGPPSNPAPAATAAPSATAAPAAPVGLTNPATAPANGSAIPAE
                     QEKSGTGPSLPPMTVPPADLALRKKWLEQRIEQLLGVLMPGRNRVGIAVLDLDNGQLL
                     YGKNAELPVNIASNVKLFTTAAALTLLGPEYRFKTVLYGDREKTDKPGEWKNLYLRGF
                     GDPWLSTEDLWKLTNELTIRGVKKIKGEVIVDDSFFDEQRVGPAFEQKNQDSAYRAPQ
                     GAVSLNSNAITVRVLPALVEGQQARVILDPPSAYIALINEARTVGSGRTSLLVDASED
                     KDAVPGHERTVVRVRGTIRVSETSGMDFHKRVAHPDLYAGYTLVDLLTKRGIQIVGKV
                     SRGTAPPSAQLLDTHYSQPLGVLVRDINKRSNNFTAEQVLKCLGADSSGKPGSWPKGL
                     QAVSRYLETLGIMPGKYQMVNGSGLFDSNRFSAVQVVTLLRAAYRDFRIAADFVASLA
                     VAGADGTIAHRLGGTPADRYVRAKTGTLNGVSCLSGYAGTPLSAQQPVRPPLAFSILV
                     NDVDETSVAKRLQDAIVEALVAFHGASTTTGH"
     gene            9029..11521
                     /locus_tag="PKL17_09715"
     CDS             9029..11521
                     /locus_tag="PKL17_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNK45049.1"
                     /translation="MSVLDRLLRAPRALLGVTLLITVLASPLLRRLNLNGDLLDLLPR
                     SSNAAQAFASYSKHMVASQELVVLVTCADPSKLTTFAEQYSRKLSELPDVAQVTHRIS
                     GQSLRYLRDHLLLLLDDDDMAELGRRLAPDALDKRAAELRGILSAPGGSAMAPIVTAD
                     PLDLIPLLTKRLGTGLKVDATSGYLRTADGTALLIKVRPRFAPLKWERDQQLLTDAGA
                     LAKSLGAEVTESDFSTGATPKVAFTGAYAFPPYFRLWIEKDMTLSTVLSVGAVLLLFA
                     LFFRSLRILPWVLLPLALAGVWTAIAATLLFGRISGVSLAFATILVAIGIDLPIQLYS
                     RLREELKRAAENGKPASDAVRHTTVSMSGAAIVATLGPAAVFLACGLSDFGGLNQLGL
                     LAGVGLILNCAAMLTVFPALLLTLPTRIWHSPDTVTKTQRSIVASIGTALAKRPRPVL
                     VLSAVLLVVSLPLVSKVRVAHDLLSIDLGEMPPARTQTELSRRFGEHQRFLVALTESS
                     DPDEALYRAEQWQKEAARLVQSKALIGFEAIGTLAPSQKTQKERRQKLASLDLPAGSQ
                     RLQKALEGAGFDSTAFADFLKLLGQDPMRIPPIKLSDLAESELGFLVRSHVAEVPGPQ
                     PRRMVALYLFAPADERITDTVARLQQIADGPIGGNITGMPLLEAQLRRLLERDLLHIT
                     LASLGLVTLLLAVYYRKLRPVLAVLLPLSLAWTLFGASLVLLGISIHLYNLLAVPLVI
                     GYGIDDHIFLVHRYQTSRDMRLSLLTAGRAVVLTTFSTVAGFMALLIAHFPGLRQFGL
                     SGALAVLLCLVAALMVLPALLALLWPERDQAA"
     gene            11619..12737
                     /locus_tag="PKL17_09720"
     CDS             11619..12737
                     /locus_tag="PKL17_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNK45050.1"
                     /translation="MQQRVFWFLCLLGLLCQTALATELSVTSISVGQGDAALIVSPTG
                     KTILVDGGPPEGTERLIQTLKRRQVSSLDLIVLTHPHLDHLGGLGQVVQTLPVRAFLD
                     AGYPSTSPPYAALMKLLSARGIAVKNATAGRNIDIGDGATLTLLGPPSPFLANTRSDV
                     NANSVVVRLSWRGRTALFTGDSEPETEQFLISHPPAGADGLRAELLKVAHHGGRYSST
                     DAFLRAVSPKLAFISAGPGNDYGHPTAEAMGRIAAVGATIYRTDLQGDLTAVSQDGQP
                     WVVKVEAAPAVMKAHHGSGPSKLPETPAPAQSTPQPPVPTTPPAAEEGYVASKNSQVF
                     HTRRCPSAERILPANRISFPTRAAAIQSGRQPGGDCKP"
     gene            12734..12994
                     /locus_tag="PKL17_09725"
     CDS             12734..12994
                     /locus_tag="PKL17_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45051.1"
                     /translation="MSGMKDPAAEPAFVDSIEAGIAKLLLRDDRGEWTAFQLPSSVLP
                     KDIKEGMWLRLFVEKSTPPHGMETAPLRKTLSAKDDGGDFSL"
     gene            complement(12996..15905)
                     /locus_tag="PKL17_09730"
     CDS             complement(12996..15905)
                     /locus_tag="PKL17_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45052.1"
                     /translation="MSKVRAERLVLIIDNDPVLSERLRAVLANYHFKVQLFADGNDLL
                     SGPQLFPELIVLCIDPKRLGWALCNRIKKTAQYGSIPMIVTSAEATEKDIDEHRKLRT
                     RADDYLLKPYSIDEMLRRVNALIGLIAAEEEEAIDETSIEELSVDDAIVEEEVVGSSE
                     PAASPPFGDGDDDFDQETESAFAALGGPEPVTTKLSPIGSRAAADPKLPGVVAQAADR
                     PTAIAAASDPGSDGPTRLIAIDGTAETAPPDARSLQQALDVAQKRLQDALAQSAYWHE
                     QRDQLASERDFLFSERDRLQRERGDLQRDQAKALTERDSLVAERNQLVIERNQLAAER
                     ELLQSKVGEPSLLAEAEARWNAEREKLVRERDQLLASFDRMAKAESGRDGEQATLLAE
                     KETLQLEQRKLATDKTLLASERERLVDDQRRLTDAEERLAAEQKRLADEQNRLTEERA
                     ELDEAKAQKGQSAALLRDREVIHLREIINKKDKDILDLRDAMDLKERQVLDSREKARE
                     QEHKRRELEEKLLSFEREQLGLQEKLDALAVDREKFLEREKGLKGRLEDAQKKLQRAD
                     EDIAALRKRLEGEAGRLLAEFAEEKQLLSTQHERRCAELLSERDRLESDAKATLAQVR
                     LDSESSLVRQKADFDALLATERAQFAASQRDSETLAREAQARLEAMYQQRLSEQIATA
                     ESALENEQRQAGEKIQKLMAERDAATAALREQLAALEAAAAKQASDDKERLSQQAVEW
                     AEKLDRAVASTRETVEAAKAQAVTALGDGHRAELATLRQAHRDELAQLATDHTQQIES
                     LLRDHAAKLRESEQSSVTELAATRARMQTQYEQLQQLLASEREVLGKQVATLEQQLGE
                     LRSRYAATESSLGSLRVRSDETKAELDAVRVALQTSKQRESELSTELASLEQQVQRAY
                     QRLVTDEDQFNRVRRALVIAMAVLDEGQGSEAPSAATPVTSSDEGPSSVTPRTEE"
     gene            complement(16066..17163)
                     /locus_tag="PKL17_09735"
     CDS             complement(16066..17163)
                     /locus_tag="PKL17_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45053.1"
                     /translation="MSRRQNDLAGTASANRARPADPLAARMSGATLIAVLLHAGLAVY
                     AYHHPMPLGPRKTGPITVDVVVRRPPPPPVPEKPPEPLPVPTAVPQPVAMQPQRATPL
                     PKELQPVERPENSAPGSSAISVPVQPPLPPGETGPTGMPKGPLVLFPKSLGGVVGGPI
                     AGAPIPKGPDRLFKDERLEEKKEPDFVLVPDKDGGYKSETRNFVARIKSDGSIDFENR
                     FPIGFQKGGTFTFDVTELAMRGAKQDPYAAEKRKFLEFSDKLRTDLRKRSIQERRESA
                     LASFSRELHAIWNSGRSAAARRRELYEKWADCSDSKEDVLGKRGRDAVEDFVRTYLPS
                     GSSDAFTSDELSDFRRERGQETAFDPYRNSK"
     gene            complement(17330..19078)
                     /locus_tag="PKL17_09740"
     CDS             complement(17330..19078)
                     /locus_tag="PKL17_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45054.1"
                     /translation="MAGEAAQGKANASSLIGKTIGGRYTIKSAIGSGGMGHVYKAIQA
                     PINREIAIKVLRVDLAGQEGVTERFKREAKAASLINHPNAITIFDFGEDDGILYLAME
                     FLAGETLRQRLRREPALTVDQALDAFESMAGALGAAHKVGVVHRDLKPDNIFIAKFDA
                     AGEVVKVLDFGLAKLLDPGTSAPEDQLTRPELRLGTPRYMAPEQALGIQPIDSRCDVY
                     ALGLLLFEMLAGRAPFTGEDGMEVLAQRLRKESPKLSAIAPNKNFSEQMDTFMARMLE
                     RDRNKRPADANEVLVQIREIRKNAQVYRVDDEEDEDPMTLNDQPAASGHYRRANVPGQ
                     PQGRATGNPARQTAAGIPASRPGQQRGTAGGMISLDQDDDLADKRTVLVDPGQDMGPP
                     PAQMMEPQRAMSLSQTPAIGQSQPHLQPYGRPTPASIPPGMSQPGSDQTAPHMSSSGV
                     QSEQKKSRRNLFIIAFVLALLAPIGALVYRWVASSNKDADNPQPVVEKPKPKPAPEPP
                     KEKPKFKLKVTATKPVNLKRDGSSIGTKAVFEEEIEKSDSKIHFTVATPGCKPAEFDY
                     VASENKEIAIKCGKKK"
     gene            complement(19336..19734)
                     /locus_tag="PKL17_09745"
     CDS             complement(19336..19734)
                     /locus_tag="PKL17_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45055.1"
                     /translation="MNFVSPAKRLLCVFGLGVLLSACGGDPAPTTTTFTTDIYPAMKS
                     ACGSCHNTGGVAAKNFIIDSASAANTYTNLTSKQLISTSAAASSKLTMVGSGGMYTPA
                     AGGAMMAHPASLPAAQKTVWETWIGNGAPQ"
     gene            20225..>22147
                     /gene="dnaK"
                     /locus_tag="PKL17_09750"
     CDS             20225..>22147
                     /gene="dnaK"
                     /locus_tag="PKL17_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNK45056.1"
                     /translation="MGKIIGIDLGTTNSVVAIMEGKEPKVIVNEEGGRLTPSVVGFDD
                     KGEILVGQVARRQSITNPENTIYSIKRFMGRKASEVGDETRRVPYKMVPANNGDAAVE
                     IRGKRYSPQEISAKVLSKLKKAAEDYLGDKVTEAVITVPAYFNDAQRQATKDAGRIAG
                     LDVKRIVNEPTAAALAYGLDKGKDHLIAVYDFGGGTFDISILEVGEKVVEVVSTNGDT
                     HLGGDDIDMRIMDWLIAEFKKDTGIDVSRDKMVLQRLKEAAEKAKIELSTVMETEVNL
                     PFLTADATGPKHLQIKVSRSKLEKMIEDLVMRSLEPTKKALADANKKVEDIGEVVLVG
                     GSTRIPMVQKLVREFFKKEPHKGVNPDEVVALGAAVQAGVLSGEVKDILLLDVTPLTL
                     GIETLGGVMTPMIPRNTTIPTKKTETFSTAADGQTTVEVVVTQGERQMARDNKILGRF
                     HLDGIPPAPRGIPQIEVTFDIDANGIVNVSARDKSTSKEQKITISGSSGLTEAEIQRM
                     VRDAESHEAEDKARKDGIEARNHLDSAVYQAEKLVAENREKLPEADRAEIEREIESAK
                     KVLEQNKDAKDAAGVAALKDGEEKLKKALYKIGEMMYRNAQGAQGAAAADPTAQGAPS
                     SGGPRGGKKDDGVIDAE"
BASE COUNT         4481 a         6786 c         6520 g         4360 t
ORIGIN      
        1 ccgacgaatc tgcaggcaat caccgccgtg ctgaatgagg cgcgggcggc agcgcggctc
       61 cagcatccga acgtcgttac gatttatcgt gtcggtgagg ttgacgatca cccggtcatc
      121 atctcggagt ttgtccgcgg ccagaacctg gatgcactgc cgaagccggt tccgtggacg
      181 cgcgccgtcg aaatcggact ggggcttgca cgcggtttgg cggcagcaca caggcgaggc
      241 gtgctccacc gagacatcaa gccaggcaac gtcatcatct cgaacgatgg cgaggtcaag
      301 ctgctcgact tcgggctggc cgagttcttc gatgcagacg gacaaggtgc cgaccccgaa
      361 gacctgatgt ggagccaggc ggcagaaatg gcgtcggtat cgggtcagta cgccaccgtc
      421 aacatgcagg tggaagacgc cgcgcgggtg atggcggtcg attctcccag ttccaacggc
      481 agcacgtcga atcccgaaga caaagagttc gtggtggtgg tcaatcgcaa gcacgttgac
      541 ccatcttcct cgacgccaga acagaaagtg ctgcggcagg gtccaccgtc acgaagcccg
      601 gtcagcgtgc gtggcaaaat gccccaagct ccggcgcagc gtttcccgac gatcccgcca
      661 agcgatagcg agccacagtt gggcccccgc agccgggtca atccatcggc atcgacaccg
      721 gaagcccaac cggaagggcg gacccccgtc cagttgcccg cctatcgtcc aacgccgcgg
      781 ccctccgccc cgaagcatca gtcacggatc gccggaacgc cgctctacat ggcgccggag
      841 atttggcgcg gcgagcccgt cacgcgagag acggacattt acgcgatggg tgccctgctg
      901 tatgagctgt gcgccggcgc accgccacac atcgaagtgc cgctgacgga gctgccccgg
      961 atcgcgtcgg aacaggatgc accgcctttg ctgcgggttg cgccgacggt tgacccccgc
     1021 tttgccgaaa tcgttgatcg ctgcctcaag cgcgacccgc gccgacgatt tgcatcgggc
     1081 gacgatctgc gcgaagcgct cgaagcgctg cgaccgcgcg aaggcaaagt cagcgccccc
     1141 gaaggaaatc cctaccgcgg cctgctcgca tttgaagccg agcaccgatc cctgtttttc
     1201 ggtcgcacaa acgaaatcgg cacgatcatc gatcgactgc gcaccgaagc gttcgtcctg
     1261 gtcgctgctg attctggcgt cggcaagtcg tcgctctgcc gcgcgggtgt actgccactc
     1321 gtcaccgacg gagcgctcgg caatggccgg acgtggtcgg tgctgctgat ggtgccgggc
     1381 cgcaatccgc tcgtgacgct gtgcgagctg cttgcgccgc ttatgcagac gcctggcgac
     1441 aagctgatgg aaaagctgcg caacgagccg accagtttgg cttgggagct gcgcaaaaag
     1501 ctcggcgaca agacaggtgt gctgattttc atcgatcagc tcgaagagct tgtgacgctg
     1561 gccgacgaac atgaagtgca aacccttggc gcggcgctcg ggcgactgtg tacgcggacg
     1621 cccggcattc gcatgctcgc cacggtgcgc agtgactttt tggcgcgggt cgcaacggtg
     1681 cccagcctcg gcgatgaact ggcgcgggcg ctttacatcc tgcgcccgat gaccagcgac
     1741 aaaattcgcg aagcgatcgt cggaccagcc catgccaaag gggtgcgctt tgaaaccgcg
     1801 gcgatggtcg atcaactgac cgagagcacc gccaaaaccg acagcggcct gccgttgctc
     1861 cagtttgcac tgaccgagct gtgggaagcc cgcaaaggcg acgtcatcac cgaggactcg
     1921 ctcgcgaaaa tcggtggtgt caccggtgcc ttggcccgcc atgccgatca agtgctgatg
     1981 agcctgcccg ccgatcagcg cgctgccgcc cgtcgcatcc tgatgatgct ggtgacgctg
     2041 gaagggacgc gcgcgcgccg aacccaggaa gagatcctga taaatccagc ggcccatgca
     2101 gccgccgaag ccctggtcaa aggccgactc atcgtcgccc gcgacaccgg ggaagcgctc
     2161 gcctatgaag tcgcgcacga agcactgatc cgcggctggg acacgctgaa gcgctggctc
     2221 gaagaagccg ccgaaagtcg tcaggtcaaa ggccggctcg aactggccgc cagcgagtgg
     2281 catcgcctgg gcaagacccg cgaagcactg tggagtgagc ggcagctcgc tgagctggtc
     2341 ctgctcgacg acagcgacgt cacagtcaaa gaacgcgcct tcattgtcgc aagccgcggg
     2401 gccgcccagc gcagtcgttt cattcgacgc gctctcatca tcgctgtgcc cgccgtgctt
     2461 acggtgattt ggctcggcgt gcgactggaa agcgcgcgca atcagcgcaa caaagtcgac
     2521 ctatatcgcg atcgcggcca gtcgattttc acggaagcga aaaaacgcgc tgcggaagcg
     2581 gcaacactgc gtcaacaagc ctatgccgct ttcgatgcca tggacaaaga tcgcggcgaa
     2641 tcactatggg caagcgcgct cgaagccgga aatgacgccg aagacctcta tacgaagtcc
     2701 gaatattcgt ataccgcagg cctcgccctc gattcaacca gggctgacgt gcgcagtgga
     2761 ataagcgatg tcctctacga tatggcttta ttatttgaac agactcacca gaaacagaaa
     2821 ttgccagatg tttttgcgcg actacagctg tacgatactg agggctcgcg gatcagcaag
     2881 ctgacagagc ctgcggttct ggacatcgct gttgagccag ccgataccga aatcgaaatt
     2941 ggccgctatg tattagacaa aaaacaaaaa taccatctgg cagatatcca ccggcttggc
     3001 gcaacgccac tcatgggcca aacgctacca cccgcctcgt atctgctgac attccgcaaa
     3061 agcggccatg aagaagtgcg ctatccgatc ttggtttccc acggcgaacg cctcaagatc
     3121 gatttccgtg tccccaaggc aggaaccatc cccgctggct acgtctacat tccgcccgga
     3181 cgattcctct tcggctccgc cgccgacgag atcatccgag cgggaacgct gtccaccgtc
     3241 ccgctccaca ccgtccagac ccagggttat ctaataagcc ggacagaagt tacgttcgga
     3301 gattttctgc acttcgcgca tactgcatcg aaggaagagc gaagcaaact tccaatcctg
     3361 tcaaatgaaa aaaactccgt atttctgcga gaatctattc cggaacagtg ggcgctaaga
     3421 gttcagcacg catcagagat aacagaaaca gcacaagatg ggacatggct ctacaagaac
     3481 aggaagaccc actcccaagc tgactggaaa aaattacctc tcggcggaat caatgcgtat
     3541 gatgcgcaaa tattcataga ctggttatct tcagccggga aactatatgg ggcacgtttt
     3601 tgcaccgagc acgaatggga acgtgcagcg cgtggcgcag acagcagaga ttttccacac
     3661 ggtaatcggc tagatccgca tgaagccaat ttcgatgaga catatggaca agacggaaag
     3721 tctgccggcc cagacatggt aggcacatat ccagattcta tgtctccttt cggaatcatg
     3781 gatatgtctg gcaatgtatt cgaatggacc cactcttctt tatcaaaagg acaaattcta
     3841 gttagaagcg gcgcgttttg gcagcccaag ttcaccgcga acatagccaa tagaagcgcc
     3901 atggactcat cgttccgtga ttcaatgctt ggcctgcgaa tctgcatttc agcaccatcc
     3961 aactaatttc atcacataaa atcaaagttc accacaaaca caacgagcac caaccaacca
     4021 acgacactca gaaataaatt gtgcgattga tcacagacaa aacgaccata atcatcaaaa
     4081 tgagaattta tctatccaaa tcaaaaatat taccgcgata ttatccaata ttttcaacag
     4141 aaaactaata actacatcta ctcctcttgt gccgccatgg tgattagctc atcgtaatca
     4201 aacagggaaa ctctcgatac gttacgatga gaattttcgt cggcttgctg ctggacagca
     4261 ggtccccacc atggaggaaa gccatgtcgt tgcgcacaaa tacaggcatc tggacactgg
     4321 ccctggcact tctcagcggg tgtccaacgc cacaacaaga tggctccgtt cctgattatc
     4381 gaacgcaggc cgtccgaacg atcaatggcg ttggactaaa tggaattggt ctcaatggca
     4441 ttggactaaa tggcattggc ctcaacggta ttggactaaa tggcattggc cttaacggca
     4501 tcggattgaa tggaaccggt ctcaacgatt ccagcctaaa tggcactgga ttaaatggaa
     4561 ccggtctcaa tgggaccggc ctcaatggaa caggcgtcac cggcctcagt ctaagctccg
     4621 taacctataa cggaaccggt ctcaacggaa ccggtctcaa tggaaccggt ctcaatggaa
     4681 ccggtctcaa cggcatcggt ctcaacggca gcgaactatt catcgcggtg aatgcccaga
     4741 ccatacttcg gggtgctacc gtcgttggca ctgaatttca ctacaaatac caggcgccag
     4801 gtagttcgac cgcggcccag tttacagtac gaatcgatgc agcttccatc gacccgaatg
     4861 cgaagattcc ttctggacag acgaacgatg tttggatcta taagctcgtt gtccgccaag
     4921 atggtatgac cccaccttcg gaatggtcgt ccctctgcta tacggaacaa ggagcgcctg
     4981 atactgcgtt gcttttgtcc aatcgctggg atcaaacgac tggaaaccgc attgatacac
     5041 caacgtttat cacgtttgcg tgccgcgact ttgcattagg aaagtgcgct cgcatgggat
     5101 atcggccttg ggccaagtcg tcatcctgca caggttctgg ctcgaatcga aaatgcagtg
     5161 atgtggcgtt ggttgatcat caccaatcct gtgttcgtat gatgcgtgct gactactgtg
     5221 gtaacggtaa gagctatact gttaatggta ctcttatcga catctacgac tacctgaatc
     5281 cgccgattca ggtgccggag acggattggg acatcgaggc gcgctggact ccgacggggg
     5341 cgatctgtat caacgagccg cggcaccctg aactggtcgg caagtggaga cggccagact
     5401 gcaacggcga cggcaagccg gattctttcc cggcctgcgg tcagtcggac aaccgctaca
     5461 gcggcaaggg tctggtggtc gatcgcttcg acgacgacca atcgggctgc ggcaagtaat
     5521 ggccgtctga cagccgccta gcgccccacc ttgcgggccg acgaaaggcc gctcagctgc
     5581 gccgtcggcc acatcctgaa accgttcacc actttccgcg ccaccgtcac gttgtcggga
     5641 tgccacagcg gcagcatcgg cagctccgcg gcgagcaagt cctgaacctt ggcgtaaatc
     5701 tctttgcgtc tctcgcgatc gatttctcga ctgccttgat ccaagagctc gtcgagcacc
     5761 ggattgcgat agcgcatccg attgagccca gcatcgaggt ttttggcgct cggaatgtag
     5821 gacgagtgga aaaagttgcg aaacaagctc ggctcggcaa tctctgccga cgacagcgtg
     5881 tacagctgaa agttgccctt tttcacgtcc gacaaaaaca ggtgaaactc gagcggcctg
     5941 atctcgacag cgacgccgat ttccgcaagc tgggcggcga tgacgcgggc aatcgcgacc
     6001 ggcagggcat ccgtcgacgt cttgtagagc agcgtcagcc gcggcttgcc acccggtcca
     6061 tctgggtcag gaaaaccagc ttcgtcgagc agccgcttcg cttcatcagg gttgtaggca
     6121 aggcttgtcg ctggctgatg gaacgcccag tgaaacggcg gcagcagact gctcgccagc
     6181 gtcgctttgc cacgcagctt ggtgcgaatg atccgctcac ggtcgattgc caacgccagc
     6241 gcccgcctca cacgcggatc acgcagccgc gcatcttcgg tattcatccc caggtacagc
     6301 agaagcgccg acggcgccgt ggttagctcc aggcgatctt tccagcgctc gaccgaatcg
     6361 aacaggagcg gcgacaccgt attttgggtc aaatccgccg atccaccgac gagcgacagc
     6421 agccgcgagt tggcatcccg aacgacccga accgtcaggt gcgcaaccgc tggcacgccg
     6481 ccaaagtact ggcgattgcg ggcaaagcgc gtcacttcac cgacctcgcc gtcgagcaca
     6541 aacggaccag ccccgatcgg catcccctgc cccggctccg ccgtcccctg tcgcatgatg
     6601 ccaaagtcca gatcggtcag atacgacgcg ggcggcttgg cataacgcag ccgcagggta
     6661 cgcggtgaca ggacttcgat cggacggatc aggccacttt cagcgaatcg ccgtcgcatc
     6721 gatggactgc cggttttttc gtcgctgagc ttgtcgatgg aataacggat gtcgtccgca
     6781 gtaaccggat ttccatcgga aaatctggca tcgcgcagcg tcacttcgag cgtcatccct
     6841 tgctcgggtg aaaccgacac cacatcgact cgctcggcca gttccagctt gggtctcagc
     6901 gagtcatcgt cgacggaaac caggcgcgcg taggaaagat aagccagctt gtagtcatac
     6961 gacgtcgtgg cgtaacgcgg atcgatgttg cgcggcggct gctcgatcag caccaccagc
     7021 tcgtcggcgg gaatctgctg gcgtcgacaa cccgtcgaaa caagcaggat cgcgatgagc
     7081 gcacgcagaa gcattgcggc aaaatgacgc aagccccggc gcgatttgct agtctgccgg
     7141 tatgttcttg gtccctcttc accgtcctgc gggcggaaag tggaacaatt tgtgggctgg
     7201 acgacgtact gcacgcctcg ctggccttgt gattgggctg tttgccgccc atccatcgga
     7261 tcgtcacgct aacccactcg ggccggacgg gccagttttc gcacaagcgc ctgcgccgac
     7321 gacgccagcc atgcctggcc caccgtcgaa cccagcgccc gccgcaacgg ctgctccatc
     7381 ggcgaccgcg gcgccagcgg ccccggttgg ccttacaaat ccagcgacgg cccccgcaaa
     7441 cggtagcgcc atcccagccg aacaggaaaa atcgggaacc ggcccgtctt tgccgccgat
     7501 gacagtgccg ccagccgact tggcgctacg gaaaaagtgg ctcgagcagc ggatcgagca
     7561 gcttttgggc gtactcatgc cgggacgcaa ccgagtcggg attgcggtgc tcgatctcga
     7621 caacgggcag ttgctgtacg gcaaaaatgc cgagctgccg gtcaatatcg catcgaatgt
     7681 gaagctgttc acgacggcgg cggcgctgac gctgctcggg ccggaatacc ggtttaagac
     7741 ggtgctgtat ggcgaccggg aaaaaaccga caagccgggc gagtggaaaa acctttatct
     7801 gcgcggcttt ggcgatccgt ggttgtcgac ggaagacctg tggaaactaa ccaatgagct
     7861 gaccatccgc ggcgtcaaaa agataaaagg cgaagtcatc gtcgacgata gcttttttga
     7921 cgagcagcgc gtcgggccag cgttcgagca aaaaaaccaa gattcggcct atcgggcgcc
     7981 gcaaggtgcg gtgtcgctca acagcaacgc gattaccgtg cgggtgctgc cggcgctcgt
     8041 cgagggacaa caggcgcggg tgattctaga tccaccgtcg gcgtacatcg cactcatcaa
     8101 tgaggcgcgc acggtcggaa gtggccgcac cagcctgctg gtcgacgcaa gcgaggacaa
     8161 agacgcggtc cccggtcacg aaagaaccgt cgttcgcgtc cgcggcacca tccgcgtcag
     8221 cgagacatcg ggcatggact ttcacaagcg cgtcgcccat cccgatctgt atgcgggcta
     8281 tacgctcgtc gatttgctga ccaagcgcgg catccagatc gttgggaaag tcagccgagg
     8341 caccgcgcct ccgtcggcgc agcttttgga tacgcactat tcgcagccgc ttggggtgct
     8401 ggtgcgcgac atcaacaagc gatcgaacaa tttcaccgct gagcaggtgc ttaagtgcct
     8461 cggtgccgac agctcgggca agccaggatc gtggcccaaa ggcttgcagg ccgtcagtcg
     8521 ctatctggaa acgctcggga tcatgccggg caaataccaa atggtgaacg gatcggggtt
     8581 gttcgatagc aaccgttttt ccgcggtcca ggttgtgacg cttttgcgcg ccgcatatcg
     8641 cgatttccga atcgccgctg attttgtcgc atcgctggcg gttgccggtg cggatgggac
     8701 gattgcacac cgattgggag gaacccccgc cgatcgctat gtgcgggcca agaccggaac
     8761 gctcaacggc gtgtcgtgcc tgtccggtta tgcaggaacg ccgctgtcgg cacagcagcc
     8821 agtccgcccg cccctcgcgt tttcgatttt ggtaaacgat gtcgacgaaa cgagcgtagc
     8881 caagcggctt caagacgcga ttgtcgaagc gctggtcgct ttccacggcg cgtcgacgac
     8941 aaccggccac taagcatccc agcgagccgc gctgccttcc gaaaaaagca gtggcgcgca
     9001 agatggcttg ggattagaac gcgaggtcat gtctgtcctc gatcgtctgc tgcgcgcacc
     9061 cagggcgctg ctcggcgtga cgctgcttat cacggtgctc gcttcgcccc ttctgcgccg
     9121 tctgaacctg aacggagact tgctcgatct gctgccgcgc tcgtcgaacg cagcgcaggc
     9181 ctttgcgtcg tacagcaagc acatggtcgc cagccaagag ctggtcgtgc tggtgacttg
     9241 tgccgatccg tcgaagctga ccacgtttgc cgagcagtat tcgcgcaagc tcagcgagct
     9301 gcccgatgtc gcccaggtga cgcaccgaat ctcgggccaa tcgctgcgct atctgcgcga
     9361 tcatttgctg ctgcttctcg acgacgatga catggccgag ctgggtcgcc ggctggcacc
     9421 cgacgcgctc gacaaacggg cagccgagct gcgcgggatt ctgtcagcgc cgggcggatc
     9481 tgcgatggcg ccgattgtga cggcggaccc gctggatttg atcccacttt tgaccaaacg
     9541 actcggcacc gggctaaagg tcgatgcaac atcaggctat ctgcgaactg ccgacggaac
     9601 cgcgctgctt atcaaagtcc gtccgcgctt tgcgccgctc aagtgggaac gcgaccagca
     9661 gctcttgacc gacgcgggag cgctggccaa gtcgctcggc gccgaagtga ctgaatcgga
     9721 tttttcgacg ggagccacgc cgaaagtcgc gttcacaggg gcctatgcgt tcccgccgta
     9781 ttttcgtttg tggatcgaaa aagacatgac gctctcgacg gtgctttcgg tcggagcggt
     9841 gctccttttg tttgcgctgt tttttcgctc gctgcgcatt ttgccgtggg tgcttctgcc
     9901 cctggcactg gcgggagtct ggacggccat cgcagcgacg ctgctgtttg gccggatcag
     9961 cggtgtttcg ctggcttttg cgacgatctt ggtggccatc gggatcgatc tgccgattca
    10021 gctctattcc cgtctgcgcg aggaactgaa acgggccgcc gaaaatggaa aaccggcatc
    10081 cgatgccgtg cgccacacga cggtgagcat gagcggtgca gcgatcgtcg cgacgctcgg
    10141 accggcggcg gtcttcctcg cttgtggact gtcggacttc ggaggtctga accagcttgg
    10201 gctgctcgcc ggggttggtc tgattttgaa ctgcgcggcg atgctgacgg tgtttccggc
    10261 gctgttgctg acgctgccaa cgaggatttg gcacagccca gacacagtga caaagaccca
    10321 gcgatccatc gtcgccagca tcggcaccgc tctagcgaaa agaccgcgtc cggtgcttgt
    10381 cctttcggcg gtgctgctgg ttgtgtcgct gccgctcgtc agcaaagtcc gtgtcgccca
    10441 cgaccttttg agcatcgact tgggcgagat gccgccagcg cgcacccaga ccgaattgtc
    10501 gcgacggttt ggcgaacacc agcgcttcct ggtggcgctg accgaatcga gcgatcccga
    10561 tgaagcgctc taccgcgccg agcagtggca aaaagaagcg gcccgtctgg tccaaagcaa
    10621 agcgctcatc ggatttgaag cgatcgggac acttgcaccg tcacaaaaaa cccagaagga
    10681 gcgccggcag aagctggcat cgctcgatct tcccgccggc agtcagcgcc tacagaaagc
    10741 cctggaaggt gcgggctttg actcgacggc cttcgcggat tttctcaagc tgcttgggca
    10801 agatccgatg cgcattccgc cgatcaagct gtcggatctg gcggaaagcg agctgggttt
    10861 tttggtccgc agccacgtcg cggaagtgcc cggtccgcag ccgcgccgaa tggtcgccct
    10921 gtatctgttc gcacctgccg acgagcgaat caccgataca gtcgctaggc tccagcaaat
    10981 tgccgacgga ccgattggcg gaaacatcac cggaatgccg ctgctcgaag cgcagctgcg
    11041 aaggctgctt gagcgggact tgctgcacat cacgctggca tcactcgggc tcgtgacgct
    11101 gctgctggcc gtttactatc gcaagctccg ccccgtgctg gctgtgcttt tgccgctgtc
    11161 gctggcctgg acgctgtttg gcgccagcct ggtgctgctt gggatatcga tccacttata
    11221 taacttactg gcggtgccgc tggtgatcgg ctacggcatc gacgatcaca tctttttggt
    11281 ccatcgctat cagacgagcc gcgacatgcg tctgtcgctt ttgacggcag gacgcgcagt
    11341 ggttctgacc acgttttcaa cggtggcggg cttcatggcg ctgctcatcg cgcactttcc
    11401 ggggctgcgg cagtttgggc tgagcggagc gctcgcagtg ctgctctgtc tcgtcgcagc
    11461 actgatggtt ttgccggctc ttttggcgct tttgtggcca gagcgcgacc aggccgctta
    11521 gaaaaaaaat cgtctaagtc cgcgaaaatc cacaactttt gcaagccgat gccgacaacg
    11581 gctcaccatg tcaaagcggc tttgggtatc gtggttctgt gcagcagcgt gtcttttggt
    11641 ttctgtgttt gctcggtctg ctttgccaga ctgcgctggc gaccgagcta tcggtgacgt
    11701 cgatttcggt gggacaaggc gatgcagcgc tgattgtttc accgacgggc aagaccatac
    11761 tggtcgatgg gggaccgccc gaagggacag aacgactgat ccagacgctg aagcgtcgcc
    11821 aggtcagcag cctcgatctc attgtcttga cccatccaca cctcgatcac ttgggcgggc
    11881 tcgggcaggt ggtgcaaacg ctgccggtgc gcgcgtttct cgacgctggg tatccaagca
    11941 cgtccccgcc gtatgctgcg ctgatgaagc ttttgtcggc gcgcgggatt gccgtaaaga
    12001 acgcgacagc cggacgaaac atcgacattg gcgacggtgc aaccttgacg ctgctcggtc
    12061 cgccgagccc gtttttggca aacacccgat cggatgtaaa cgccaattcc gtcgtggtgc
    12121 gactttcctg gcggggtcga acagcgctct ttaccggcga ttccgaaccg gagaccgagc
    12181 agtttttgat ttcgcatccg ccggcgggag cggacggtct gcgcgccgag ctgctcaagg
    12241 tggcccacca cggcggacgc tattcgtcga ccgatgcctt tctgcgagcc gtgtccccga
    12301 agcttgcctt catatccgcg ggaccgggca acgactacgg acatccgacg gcagaagcga
    12361 tgggacggat tgcggcggtg ggcgcaacca tctatcgaac cgatctgcaa ggcgacctga
    12421 cagcggtcag tcaggatgga cagccgtggg tcgtcaaagt cgaggcggcc ccggcggtca
    12481 tgaaagcgca ccacggatcg ggaccctcga agctgccaga aacgccggcc ccagcccagt
    12541 cgacaccgca acctccagtg ccaacgacgc caccagcggc ggaagaaggc tatgtggcca
    12601 gcaaaaactc ccaagtgttt cacacgcgcc gctgtccaag cgccgaacgc atcttgcccg
    12661 ccaatcgaat cagctttccg acccgcgccg cggccataca gagcggacgc cagcctggag
    12721 gagactgcaa gccatgagtg gaatgaaaga cccagcggca gagccagcgt ttgtcgattc
    12781 cattgaagcg gggattgcga aacttctgct gcgggatgac cgtggcgagt ggacggcctt
    12841 tcagttgccg tcgtccgtgt tgcccaaaga catcaaagaa gggatgtggc tgcgactgtt
    12901 cgtcgaaaag tccacgcccc cccacggcat ggaaaccgcg ccgctgcgaa agacgctatc
    12961 ggccaaagac gacggcggcg acttttcgct ctaagttact cttcagtcct gggggttacg
    13021 ctgcttgggc cctcgtcgct cgatgtaact ggcgtcgctg cgctcggcgc ctcactgccc
    13081 tgcccttcgt cgagcaccgc catcgcaatg accagcgccc ggcgaacccg gttgaactgg
    13141 tcttcgtccg tcaccaaacg ctgataggcc cgctgcacct gctgttccag actggccagc
    13201 tcggtggata gctcgctttc gcgctgcttg gacgtctgta gcgcgacacg caccgcatcc
    13261 agttcggcct tggtttcatc tgagcgaacc cgcagactcc ccaggctcga ctccgtcgcg
    13321 gcatagcgac tccgcagctc accgagctgc tgttcaagcg tcgccacttg tttgcccaac
    13381 acctcgcgct cgctcgccaa cagctgctga agctgctcat actgcgtttg catccgggcc
    13441 cgcgtcgccg caagctcagt taccgacgac tgctcgctct ctcgcagctt ggcggcatgg
    13501 tcgcggagca gcgactcgat ctgctgggtg tgatcggttg caagctgggc cagctcatcg
    13561 cgatgtgcct ggcgcagcgt cgcgagttca gccctgtgcc catcgccaag tgcagtcacc
    13621 gcttgcgctt tcgccgcttc gacggtttcg cgcgtgctcg ccaccgcccg atccagcttt
    13681 tcagcccact cgacagcttg ctgtgacagc cgctctttgt catcgctggc ttgcttggcc
    13741 gcagccgctt ctagcgcagc caactgctcg cgcagcgcag cggtcgccgc atcccgctct
    13801 gccatgagct tctggatctt ttcgcccgct tgccgctgtt cgttttcgag cgccgattca
    13861 gcggtcgcga tctgctcgct gagccgctgc tgatacatcg cttcgagccg cgcctgggct
    13921 tcacgcgcca gggtttcgct gtccctttgc gacgccgcaa actgtgcccg ttccgttgcc
    13981 aagagcgcat caaagtccgc tttttggcga accagcgaag actccgaatc cagccgcacc
    14041 tgggcgagcg tggctttggc atccgactct aggcgatccc gctcggacaa cagctctgca
    14101 caacgccgct catgctgggt cgaaagaagc tgtttctctt cggcgaactc ggccagcagc
    14161 cggcccgctt cgccttccag ccgcttgcgc agcgccgcga tgtcttcgtc cgcccgctga
    14221 agctttttct gcgcgtcttc gagccgacct ttgagcccct tttcgcgctc caaaaacttt
    14281 tcgcgatcga ccgccagcgc atcgagcttc tcttgcaggc cgagctgctc acgctcgaac
    14341 gaaagcagct tctcttctag ctcacgacgc ttgtgctctt gctctcgggc tttttcacgc
    14401 gaatccagaa cctgccgctc tttgagatcc atcgcgtcgc gcagatcgag gatgtctttg
    14461 tcctttttgt tgatgatctc gcgcaggtgg atgacttcgc ggtcccgcaa aagcgccgcg
    14521 ctttgacctt tttgcgcctt cgcctcatca agctcggccc gctcctcggt cagccgattt
    14581 tgttcgtcgg ccagtcgctt ttgctcagcc gcaagacgtt cttccgcatc ggtcagacga
    14641 cgctgatcat cgacaagccg ttcgcgttcg ctcgcaagca gcgtcttgtc cgtcgccagt
    14701 ttgcgctgtt cgagctggag cgtttctttt tcagcaagca gcgtggcctg ctcaccgtca
    14761 cgacccgact ctgctttggc cattcgatca aagctggcga gtagctgatc gcgctcgcgc
    14821 accagctttt cacgctccgc attccagcgc gcttccgctt cagcgagcag cgacggttcc
    14881 cccactttgc tctgcaaaag ctcacgctcg gcggcaagct ggtttcgctc gatcacaagc
    14941 tgattgcgct cggccaccag gctgtcgcgc tcggtcagcg ccttggcctg atcacgttgc
    15001 aaatccccgc gctcgcgctg aagtcgatcg cgctcgctaa acaggaagtc gcgctccgac
    15061 gcaagctgat cgcgctgctc atgccaatag gccgactgcg ccagcgcgtc ttgcaagcgc
    15121 ttttgcgcca catcaagcgc ctgctgaagc gatcgcgcgt cgggcggcgc cgtttctgcc
    15181 gttccgtcga ttgcaatcaa tcgcgtcggt ccgtcgctgc caggatcgct ggccgctgcg
    15241 atcgcggtcg gtcggtccgc cgcttgcgca acgacaccgg gcagcttggg atcggctgcg
    15301 gcacgagatc cgatcggcga cagcttggtc gtcaccggct ccggaccgcc aagcgcagca
    15361 aacgccgact ccgtttcttg atcaaaatcg tcgtcgccat cgccaaacgg aggacttgcc
    15421 gcaggctcac ttgaaccaac gacttcttct tcgacgatgg catcatcgac cgaaagctct
    15481 tcaatcgaag tttcgtcgat ggcttcttct tcttccgccg caatcagccc aatcagcgca
    15541 ttcacccgcc gcagcatttc gtcgatgctg tacggcttga gcaaatagtc gtctgcgcgc
    15601 gtccgcagct tgcgatgttc gtcgatgtcc ttttccgtcg cttctgccga cgtcacgatc
    15661 atcggaatcg agccgtactg cgcggtcttc ttgatccgat tgcacagcgc ccagcccagc
    15721 cgctttggat cgatgcaaag aacgatcagc tcgggaaaga gctgcggccc cgacagaaga
    15781 tcatttccgt cggcaaatag ctgaaccttg aagtggtaat tggcgagcac ggcacgcaac
    15841 cgctcactca gtaccggatc gttgtcgatg atgagcacca agcgctcggc cctgaccttg
    15901 ctcatgttca ccccttcgct ttgccctcgc gggacatcca tgcttcctat cacagagtga
    15961 cgggcgctgg gcagtcgaaa cttgccgatc caaacggcgc gaagtgtgac aaagagcacc
    16021 ggcatcagcc gatgcagcga cggtgatcac aactggcagg cggtattact tgctgtttcg
    16081 gtacggatca aacgcggtct cttggccacg ttcgcgacgg aaatccgaca gttcatcgct
    16141 cgtaaaagcg tcggaactgc cggaaggaag ataggtgcga acaaaatcct cgacggcatc
    16201 acggccccgc ttgccgagca cgtcctcttt tgaatcggaa caatcggccc acttttcgta
    16261 cagctcacgc cgtcgcgctg ccgccgatcg tccactgttc cagatcgcat gcagctcacg
    16321 cgaaaagctg gccagcgccg actctcgtcg ctcctggatc gatcgcttgc gcagatcggt
    16381 tcgcagcttg tcggaaaact cgaggaactt ccgcttttcg gcagcgtagg gatcttgctt
    16441 tgccccgcgc atcgccagct cggtcacgtc gaacgtgaag gtgccgcctt tttgaaaacc
    16501 gatcgggaag cggttttcaa aatcgatcga gccgtcggat ttgatgcgcg cgacaaagtt
    16561 ccgcgtttcc gacttgtagc cgccgtcttt gtcgggaacc agcacaaagt caggctcttt
    16621 tttttcttcg agccgctcgt ctttgaacag ccgatcgggg cctttgggaa tgggcgcgcc
    16681 agcgatggga ccaccgacga ctccaccgag cgatttggga aacagcacga gcgggccttt
    16741 gggcatgcca gtcggtccgg tttcgccggg tggcagcggc ggctgtaccg gaaccgatat
    16801 cgccgagctg cccggcgcgc tgttttccgg tcgctccacc ggctgaagct ctttcgggag
    16861 cggcgtcgca cgctgcggct gcatcgcaac tggctggggc accgccgtcg gcacaggcag
    16921 tggttccggc ggtttttccg gcacaggcgg cggcggcggc ctgcgcacca cgacatccac
    16981 cgtaatcggc ccagtcttgc gtggaccgag cggcatcggg tggtgatagg catagacagc
    17041 caacccagca tgtagcaaca ccgcaatgag cgtcgcaccg ctcattcgcg cggccagcgg
    17101 atctgcgggt cgcgcccggt ttgccgacgc tgtgccggcc aaatcgttct gtcttctgct
    17161 catcgtccgc gtactatcgc caactattac acaggtttcg ctgcgacgca gctttctcga
    17221 atcagcgaat cgggggcaaa gactcgattc gtgaaaacga accaggtcgc ccaaatagca
    17281 ggtcttgatg gatgaagggg agcaaactac cgtcgaaggc gagccagtct tacttctttt
    17341 tgccgcactt tatcgcgatc tctttgtttt cgctcgcgac gtagtcaaat tcggcgggct
    17401 tgcagccagg cgtggcgacg gtgaagtgaa tcttgctgtc gctcttttcg atttcttcct
    17461 cgaaaacagc tttggtgcca atcgagctgc catcgcgctt gaggttcacc ggcttggtcg
    17521 ccgtgacctt gagcttaaac ttcggctttt ctttcggcgg ctcgggagcc ggcttcggct
    17581 tgggtttttc gacgaccggc tggggattgt cagcgtcttt gttgctgctg gcgacccagc
    17641 gatagaccag cgcgccaatc ggggccagaa gcgccagcac aaacgcgatg ataaacaggt
    17701 tgcgacggct cttcttctgt tcggactgca cccccgacga agacatgtgg ggtgcggtct
    17761 gatccgatcc cggctgcgac atgcccggcg gaattgaagc cggcgtcggg cgcccatagg
    17821 gctgaagatg cggctgagac tgcccaatcg ccggcgtctg cgacagcgac atcgcccgct
    17881 gcggctccat catctgcgcc ggcggcggtc ccatgtcttg tcccgggtcg acgagcacgg
    17941 tgcgcttgtc cgccaagtcg tcgtcttgat cgaggctgat catgccgccg gcagtgcccc
    18001 gctgctggcc gggacgagac gccgggattc ccgctgccgt ctgtcgagcc ggattccccg
    18061 tcgcccgtcc ttgcggctgt ccgggcacat tcgcgcgacg atagtgtccc gatgctgccg
    18121 gctgatcgtt cagcgtcatc ggatcttcgt cttcttcgtc gtcgacccga tagacctgag
    18181 cgtttttgcg aatctcgcga atctggacca gcacttcatt ggcgtcggca ggccgcttgt
    18241 tgcgatcccg ctcgagcatg cgcgccataa aggtgtccat ctgctcgctg aagtttttgt
    18301 tgggcgcgat cgccgacagc ttgggcgatt ctttgcggag ccgctgcgcc agaacttcca
    18361 tgccgtcttc gccggtaaac ggcgcgcgcc ccgccagcat ttcaaacagc agcagaccca
    18421 gcgcatagac gtcgcagcgc gagtcgatgg gctgaatccc aagtgcctgt tccggcgcca
    18481 tgtagcgcgg cgtgcccagt cgcagctcgg gccgcgtgag ctgatcttcg ggggccgacg
    18541 tgcccggatc gagcagcttg gccagaccga agtcgaggac tttgaccacc tcacccgccg
    18601 catcaaactt ggcgatgaag atgttgtctg gcttcagatc gcggtgcacg acgccaactt
    18661 tgtgcgcggc gccgagtgct cccgccattg actcaaacgc atcaagcgct tggtcaacag
    18721 tgagtgcagg ttcccgccga agccgctgtc gcagcgtctc tcccgcgaga aactccatcg
    18781 ccaggtagag gattccgtca tcctcaccaa agtcgaagat ggtgatggcg ttggggtggt
    18841 ttatcagact ggcggccttc gcttcgcgct taaagcgctc ggtgacgcct tcctgtcctg
    18901 ccaaatccac ccgcaagacc ttgattgcga tctcgcggtt gatcggcgcc tggatggcct
    18961 tatagacgtg gcccatcccc ccagagccga tagccgactt gatcgtatac cgtccgccga
    19021 tggttttccc aatcagcgag gacgcattcg ctttcccttg agcagcttcg ccggccatcc
    19081 tgatgccaaa gccatcttat ccgatttcag gatttcaagg ctatagggca tcttggcaaa
    19141 aatgcgtggt ggttctctac gtttcgtttg cgtcgccagt tgtgatgacg ccacgcggaa
    19201 tgggataggt gcctacaagt gacccggtga ccaaaccgcg cgcccggcta ccgcaggctg
    19261 tctggccggg cctaggaaaa gttgtgaggg tgggaagaaa cagaaggaac cgacaggacg
    19321 gtcgaatcgg tcaggctact gcggagcgcc gttgccgatc caggtctccc aaaccgtctt
    19381 ctgcgcggcg ggaagactgg cagggtgcgc catcattgcg ccacccgctg ccggagtgta
    19441 cataccgccc gagcccacca tcgtcagctt cgaagacgct gccgccgagg tgctgataag
    19501 ctgcttgctg gtcaggttgg tataggtgtt cgcagcgctc gccgagtcga tgatgaagtt
    19561 cttggcggcc actccgccgg tgttgtggca agaaccacat gcactcttca tcgctggata
    19621 gatgtcggtg gtgaacgtgg tggtggtggg tgccggatcg ccaccgcagg ccgacaaaag
    19681 gacaccaaga ccgaacacac agagcagccg cttcgcaggg gacacaaaat tcatggacac
    19741 tctcctcgtt gtttggttgt gaggcttagg ttaagacccg tcttctcggg aagatagtcg
    19801 tcgaaaaacg attggcaccg acaccgattt gccacaagcc cgacccgcga acccgcggca
    19861 tccgcgccac gttggcttca aagcacacgg tcagttggtg gttcgtctgc ggcattttcg
    19921 tcatcactcg gccacgttgt cttgttagca tcacattctg ccgagcgagt caaacacttc
    19981 gcccacttta ccgtggaatg ctgcgctgtg tcatcccccg caaacccccg ccgcgcaaac
    20041 gggacaagct ggactacatc ggcgtcgatc caagagcgac tttcagcggt cccgagcgat
    20101 gcacgcgatc gactgcaaga agcggacttg acggtttgac atgcgtgctt actttcgctg
    20161 ctgaaaatcg aaacactcga aacgggatag caaacaacac cgttcgcccg gaggcacaag
    20221 gaacatggga aagatcattg gtatcgacct gggtacgacc aactcggtcg tcgccatcat
    20281 ggaaggcaaa gagcccaagg tcatcgtcaa cgaagaaggc gggcgactga ctccgtccgt
    20341 ggttggcttc gatgacaagg gcgagatcct ggttggacag gttgctcgtc gccagtccat
    20401 taccaacccg gaaaatacga tctactccat caagcgcttc atgggccgca aggcatcgga
    20461 agtcggtgac gagacccgtc gggttcccta caagatggtg cccgcgaaca atggtgatgc
    20521 tgctgttgag attcgcggca agcgctactc gccgcaggaa atctcagcca aggtgctgag
    20581 caagctcaag aaagcggcgg aagactacct cggtgacaaa gtgaccgaag cagtcattac
    20641 ggtgccggcg tattttaacg acgcgcagcg ccaagcgacc aaagacgcgg gccgcatcgc
    20701 gggtctcgac gtcaagcgca tcgtcaacga gccgacggca gccgcgctgg cctacggtct
    20761 cgacaagggc aaagaccacc tgatcgcggt ctacgacttc ggcggtggta cgttcgacat
    20821 ctcgatcctg gaagtcggtg aaaaggtcgt cgaagtggtc tcgacgaacg gcgacaccca
    20881 cctcggtggc gacgacatcg acatgcgcat catggattgg ctgatcgccg agtttaagaa
    20941 agacaccggc atcgacgtca gccgagacaa aatggtgctc cagcggctca aggaagcggc
    21001 ggaaaaggcc aaaattgagc tttcgacggt gatggaaacg gaagtcaacc tgccgttcct
    21061 taccgcggat gcgacggggc ccaagcacct tcagatcaaa gtctcgcgca gcaagctgga
    21121 gaaaatgatc gaagacttgg tgatgcgctc gcttgagccg acgaagaaag cgctcgccga
    21181 cgccaacaaa aaagtcgaag acatcggtga agtggtgctc gttggtggtt cgacgcgcat
    21241 cccgatggtc cagaagctgg tgcgcgagtt cttcaagaaa gaaccgcaca agggcgtaaa
    21301 ccccgacgag gtcgtcgcac tgggtgctgc agtgcaggcc ggcgttcttt cgggtgaagt
    21361 gaaggacatc ctgcttctcg acgtgacgcc gctgacgctc ggcatcgaga cgctgggtgg
    21421 cgtgatgacg ccgatgatcc cgcgcaacac caccatcccg acgaagaaga cggagacgtt
    21481 ctcgacggcg gcggatggtc aaacgacggt tgaagtcgtg gtgacgcagg gcgagcgcca
    21541 gatggcgcgt gacaacaaga tcctcggacg cttccacctg gacggcattc cgccggcacc
    21601 acgcggcatt ccgcagatcg aggtcacgtt cgacatcgac gccaacggca tcgtcaatgt
    21661 ctcggcgcgc gacaagagca cgagcaaaga gcagaagatc accatcagcg gcagctcggg
    21721 tctgacggaa gcggaaatcc agcgcatggt ccgtgacgcc gaatcgcacg aagccgaaga
    21781 caaggcgcgc aaagatggca tcgaggcacg caatcacctc gacagcgcgg tctatcaggc
    21841 tgaaaagctg gtggcggaaa accgcgagaa gctgcccgag gcggatcgcg ccgaaatcga
    21901 gcgcgaaatc gagtcagcca agaaagtcct cgagcaaaac aaggacgcca aggatgccgc
    21961 tggtgtcgct gcgctcaagg atggcgaaga gaagctcaag aaggcgctct acaagatcgg
    22021 cgagatgatg taccgcaacg cgcagggagc ccaaggtgca gcggctgccg acccgacggc
    22081 gcaaggagcc ccctcatcgg gtggaccgcg cggcggcaag aaggacgacg gcgtgatcga
    22141 cgccgag
//
LOCUS       DAORGN010000070        22109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_939_length_22109_cov_5.797633, whole genome shotgun sequence.
ACCESSION   DAORGN010000070 DAORGN010000000
VERSION     DAORGN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 22109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22109
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_22109_cov_5.797633"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="PKL17_09755"
     CDS             <1..1995
                     /locus_tag="PKL17_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45057.1"
                     /translation="RSGFSSSLLDCDIPPPAEVEGYEIKKPLGRGAYGTVWLAAQKNT
                     GKQVAIKFYAHAGGLDLVLLRREVESLAFLYTERDVVQLLAVGWDHEPPYYVMEYLPH
                     GSLEERLRNGPLPLTDAVTITKVVARALQKAHGRGILHCDLKPANVLFDGNGKPRLCD
                     FGQSRLSTDHKPSLGTFFYMAPEQADLLAVPDARWDVYALGAMLYAMLVGAPPYRDGP
                     LQPLLRRATTLPERLRVYREFLLCSKPPDIQHHRRGIDAALVKIVSRCLATDPAQRYP
                     NVQAVLTALDLRATRQQRRPLWLLGALGPALLLVVTSLIALKGVRVAVQTTSVAVTQR
                     ARESSQFAARFVAETAGREIDRRWSVLEQAALDPALRQELTLLPPGHTAPSPTLQRFI
                     EAQSRQNADLAAASWFVTAVSGFQLARAPYSDTIGRNWAFRDYFHGLGRELPRDTPPQ
                     TIRPITQPHRSAVFPSQATRTRLVAFSVPIWSPTEPRKVVGVLGMTVELGGFGELRPG
                     RGASERQIAVLVDGREDWTGQKGLVLQHPQLSALLAQKRPLPPYRLDDQHIKTIEALR
                     RAAADGLPLPLDLLFDAHYRDPVSGSYGGEWLLTMRPVLSRQRDTGWVVLVQERLTDI
                     TQPITALGHSLLRIGMVGLLLMAVVWLLLWWRVSRSFVRT"
     gene            2088..2963
                     /locus_tag="PKL17_09760"
     CDS             2088..2963
                     /locus_tag="PKL17_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK45058.1"
                     /translation="MTLGLVCDACDTLSPLSATLCSVCGAALGSKSVPGPTSSPRSCP
                     NCASEIPGQHRFCGFCGTRTDGVDLRPVWHSGITTKTRLTLVGQDGQEGLSFPLTEGE
                     HVAGRLEGAIMFPEDRLLSPRHATFLLQSGKLWVRDDGSHNGVFIKLRTPKIVPSGTV
                     LLIGEQLLRIETAPPDVLPYPDQEGTYFYCSPHRPARLTMVQILVGGDIGLIYRSRGD
                     TVTIGREMNDVNFPEDPFISGRHAQIVALEDGRFQVTDLGSKNGTYEKVPRMVQLASG
                     DQLFLGQQLLRVEMG"
     gene            3034..3738
                     /locus_tag="PKL17_09765"
     CDS             3034..3738
                     /locus_tag="PKL17_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK45059.1"
                     /translation="MLRACQYCGNALSVESVPRGALRGRLVLVRPDGSEGGAHPLFEG
                     INVVGRGSGPLFDADSFLSPVHLRCEIAGGRFVVRDAGSLNGVFLKLTEEESLFEGAI
                     LRIGQELLRYDSMRPPKPLPDGTEIMGSPNAGYWGRLTMIIGRTQDGRIIEGTSFPLA
                     GEAVVIGRERGDILFSEDGYVSGTHARFSFRQGRPYLQDLASSNGTYLRIPAERAVVP
                     GTFLLLGQQLFRLMPA"
     gene            complement(3752..4039)
                     /locus_tag="PKL17_09770"
     CDS             complement(3752..4039)
                     /locus_tag="PKL17_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNK45060.1"
                     /translation="MTTWKCRKCKYVYDPKLGDPDGGIPPGTPFDQIPDSWCCPICGA
                     TKRDFFVLSTAPPAPANDRIAAPAVLAQPQSVRQPQSAGFTVGSLKGKPQR"
     gene            complement(4102..4512)
                     /locus_tag="PKL17_09775"
     CDS             complement(4102..4512)
                     /locus_tag="PKL17_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNK45061.1"
                     /translation="MKAHRNAQAASLCALLLFGCQRTTGLPPIEPPPVVSEAAKAQGA
                     KLFAEHCVRCHGVSGWGDGPEALANKKVRVQDLGDPVWQSNVSNGRLVAAIVRGGPGV
                     RKSAQMPAFPLLAKNREEIDGLVAHIRSLARTTP"
     gene            complement(4509..5546)
                     /gene="glk"
                     /locus_tag="PKL17_09780"
     CDS             complement(4509..5546)
                     /gene="glk"
                     /locus_tag="PKL17_09780"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNK45062.1"
                     /translation="MTLLAGDLGGTKTLLALFDDAGTLLHKQRFASQQHPDLESMVTA
                     FLAATAKDLHPQVMALGVAGPVAKHAGFETCDVTNLPYRIDGRSIAARFGFASVRLVN
                     DFYAVAAAVSVLARGGSFPGLSLCSLHPLAHAEPGSPIAVLGAGTGLGEALIAFHGDE
                     PVILPTEGGHTDFAPHDDLEVQLWRYLQRRHPDHVSQERLVCGPGIATLYAFFRDERI
                     GTESPQVAAELARCDDPARVISRHALAKTDPLCEQVMDRFVMLFGAEAGNLALKSLAR
                     GGVYLAGGIAAKNLPLFTSGLFVSHFARKGRFSGLLSSIPIHVVQGEEIGLFGALTIA
                     KSIQQGQIRSS"
     gene            complement(5553..6815)
                     /locus_tag="PKL17_09785"
     CDS             complement(5553..6815)
                     /locus_tag="PKL17_09785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK45063.1"
                     /translation="MTMKKSSYDVIVAGGGPAGSTTASFLRKHGHSVLVLEKERFPRF
                     HLGESLLPCGGEVWDELGVSDEMKQRYIMKPGARFIHNESGLTQTYYFSDAIRPDHPF
                     AYEVPRADFDDLLLRRSESLGAEVLEETEVRDVRFFESGVDVTIRRADGSIEVPRAQV
                     FVDATGRDTFLGTRFGLKVSDDLVTTNLACFTHYENCNRQTGKDEGNITMVLFDGGWW
                     WFIPFKGTVTSIGCTLQKHFTVARRGYSAEQMFMAALEATPVMQELLRGATRVRPIDT
                     TGNWSYRCKQFYGDRFLLVGDSAAFIDPLFSTGVLMAVTCGKYAAKAIHEALLAGDCS
                     AERFAGYQTAALQGADLFKGLVHEFYARHLRPLLVRSAAHRATYTAITSALAGDVYRP
                     AMWQGLFREGFSERAIDSMGLPRQTGES"
     gene            complement(6903..9068)
                     /locus_tag="PKL17_09790"
     CDS             complement(6903..9068)
                     /locus_tag="PKL17_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNK45064.1"
                     /translation="MFESVFHSPLALFIAQAVLIIAVARGLGLVVRFVGLPMVISEIL
                     TGIVLGPSLLGWLWPGVSAALFPKNSLGLLQIFAQVGLVLFMFLVGLELDTKMVKKHG
                     RASLLISIAGMAVPFSIGGGTATLLYDRLSSPDVRPLSFALFLGTALSITAVPVLARI
                     LSATQMLHSKVGTVALTCAAVNDVLAWCLLAFVVATARSAGMSSSLKPTLWTLVYVAV
                     MWFVVRPLLRRLGQRGAGREGLSQNLVAAVFLLLFLSSFTTELIGIHALFGAFLFGIL
                     LPKEGGFAAKLADRLEDFAVVFLLPLFFAFSGLRTQVNLLSQAHLWVMFALFLSLAVV
                     GKFGACTLAARVSGLSWRESAAVGVMMNTQGLMELVVLNIGLDLGVISPTMFTIMVLV
                     ALVSTVSTTPLLKLIYPQSLISAELAEPRAVDAFCLLLCVSYPATVPGMIKLSSALTL
                     RSRERSRIYALKLQRVPERPSHYVADRQDPQAASQVLSPVLALAEEAQLKVRPIAFVS
                     SHLARDIANVSEVKGANLVLLGWHRPLFNRAALSGTVGEVLEDARADVGVLVDRGLQN
                     ITRVLVPFHRTAQDRAALQLARRLLDQAGASITLLHVVDDDQTAPSQPDVDQLLREVF
                     GTDAANVTVKRVLAAEPAEAALAEIRSETASPYDLVLVGVGRDWGLEARQFGIQQEFL
                     IRQCPTSLLIVRGYDPSLHGDRAKTSAESSQSPDHVQPA"
     gene            9225..10274
                     /locus_tag="PKL17_09795"
     CDS             9225..10274
                     /locus_tag="PKL17_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45065.1"
                     /translation="MHRFVSRSLRKKSLVFTLAPLLASGCLPDPDIKVPDFKPSGIVD
                     LGKAPLDMTTLPDAGRSRFVPDGAVPTGQTLRAIYVDAGGQIFVAGHGGTILRRSGTV
                     WTVEQAIDGAAPITSNLYGLAGSGSELYAVGEAGVVVKRSGDKWLREGKTLAVTASLF
                     ATTITSAGDVYAIGDLGTVLRKPMGGTWTKEAVSAGLATAEFRGVAETKNDELTAVGL
                     GGVIAQRSGSAWNSDALAIDSADRVNFYGVAKTSEAVFIAGDSNRVLRRDADKWRREP
                     TGNLTGPSANLFALSASGQDLIVAGAAGLIERRSAQSKTWSIEDSGTTVMLSALSNVS
                     PLRAVGAQGTMVVQK"
     gene            10271..10945
                     /gene="cmk"
                     /locus_tag="PKL17_09800"
     CDS             10271..10945
                     /gene="cmk"
                     /locus_tag="PKL17_09800"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNK45066.1"
                     /translation="MIHPVIAIDGPAGAGKSTVARELARRLGFFLLDTGAIYRSLALF
                     AQQNGISWADGAKLGTLAETLPIRFGRGSEDGAVFLGEQVVTTAIRSPEVSLGASQVS
                     AHPEVRAMLLGLQRQLARSGPCVVEGRDIGTVVLPDAPLKIFLTASPEVRAQRRQKEL
                     EARGVKASAEATRREQEERDRRDLTRTTAPLKCADDAVLFDTSEHSLEQVLDALVSVA
                     HERLKL"
     gene            complement(11359..14298)
                     /locus_tag="PKL17_09805"
     CDS             complement(11359..14298)
                     /locus_tag="PKL17_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK45067.1"
                     /translation="MSALRRVFPSAVSAAVLLVASAQTFAAPPAATGLRPPAAAATPA
                     ANPASRDPFSLTAERFTLPSGMTVLLRRDPSVPRVVVSLWFSVGSRDEAPGRTGFAHL
                     YEHLMFMGTKNVPDNRFDVLMESEGGGNNATTSTDRTFYFDFGPSHLLPTLLWLEADR
                     LTNLPETMTDEKVSLQREVVRNERRQSYENRPYGMAELRVSSAMFPAGHPYSWPIIGS
                     HEDLIAASTSDVKQFFYKYYTPSNATLAVVGDFNPEEARKLISTYFGWMPKRPKPAAP
                     SLQNGVPEMVVPVEQSVTIEDQVTLPRVYVLWHAPPVGTPDFPDSVLVADLLGKGKAS
                     LLYRKLVVEQKLAQAVEAEVDPQQLGSVMSLSVTAQQGVTTEQLTAAVEKELATFVSA
                     PPTAEEIARARNLRLTELARDIETPLGQSLWLQQLFAWSGDQNGLPQLVGKFRMATPQ
                     SLQKRALDLGVGDRGRRLQIQVVPRKGPPPAPPVAEASKPKPQPFYPGGKAPKPQPLP
                     GPMRAPFDLSKQPKSLALRPPTPPTSAHFRVHGVDVTLVQRSGTPLIETAVAIPTGEA
                     DAVAAKSGLAESVAAMLSEGSGQRAGTDWAAAMQAAGATWKTQVSSDTTLLSMGVLSQ
                     TYAEAAPLFYEALLRPRFDDKEWTRVRGERLAALEQKNDEPPQVADRVLRASLFGASE
                     PYGRSPQGTAASLQKLSVSDLKAFHQSFYPSKNWKIVIAGDFEPSQAVADHLLGVLGR
                     LPFPAGSPSLVRPPRTPPKPQRGLIFVDRPGAPQAELRFANLVPGALDPERPAMDMAN
                     VLLGGMFTSRLNQNLREKHGYTYGARSQVVRMDGLGYWVSMAAVRSDVTTESTVELRN
                     EVTRMRDEVCSAEELQRSRAGAIQRLVMQSERTAGLAQLYATFARNRLPMDEFAKLVS
                     GAQTVTAAKLQEVGRSRLHPEDATIVIVGDVQKLLPKLPQALGLSPADVQRRDVEGKP
                     LPATPTK"
     gene            complement(14298..14600)
                     /gene="yhbY"
                     /locus_tag="PKL17_09810"
     CDS             complement(14298..14600)
                     /gene="yhbY"
                     /locus_tag="PKL17_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNK45068.1"
                     /translation="MTNRQRTVLRGLGHHLNPVVQLGKDGISEGLLAAVATVLEQHEL
                     IKIKLLETAPGDRHELAEQLAKDCRAALVQVIGRNLLLYRPRPLTDPRPSLEMELA"
     gene            14822..15424
                     /locus_tag="PKL17_09815"
     CDS             14822..15424
                     /locus_tag="PKL17_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45069.1"
                     /translation="MKKTTFDVLRDGKEGPLLFACMQRPDDREARLRYLQFLQATGDP
                     RAEPLRLQVQLSTLTESGSPQTAQIEETRRQLRKLVPTLDRVWWAALARSPETFHCGQ
                     ARSAPSLHVRFRFECPQNWDLLTETGDPSQRFCTVCAQTVHFADTVSKAEHLARQGAC
                     IAVPSALVDKTVRKVTELAVGRPDWKKMWADRIFGPISDD"
     gene            15488..16648
                     /locus_tag="PKL17_09820"
     CDS             15488..16648
                     /locus_tag="PKL17_09820"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HNK45070.1"
                     /translation="MQTIAPASSSLPQRLFTPGPLSTTATVKAAMLVDLGSRDPFFLR
                     TVAELRTELLAIAQVSEPEFAAVPIQGSGTFAVEATLGSAVGKTGGLLILSNGAYGDR
                     LADIARSVGIPHVVHRFAETEPLDPHVIATAQRKAPHLSHLAFVHCETSTGQINNIDE
                     VSITARAAGLSVICDAMSSFGAIATPLDRLPIDFLVTSPNKCLQGVPGCGLVLLRRSA
                     LRKGQAHSMSLDLWAQLDGLDRSGQFRFTPPTHVLLALRQALRELAEEGGQEQRRARY
                     QANSNLLADGMAKLGFRTLLPKAAQSPIISAFYYPDDARFDFAAFYRSLGERGLVIYP
                     GKVSHAACFRIGTIGDLYPDDIRELLTAIPLVLSTQGISVPLSATPSEPRSC"
     gene            16642..17481
                     /locus_tag="PKL17_09825"
     CDS             16642..17481
                     /locus_tag="PKL17_09825"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HNK45071.1"
                     /translation="MLDAHSPDSRVPFVPLKAVIFDWAGTICDFGSLAPVQALQSLFA
                     RHGVPITCAQARGPMGRAKRDHITDILAIPDVARRFSEVHGKPPQSADIDRLFADFLP
                     IQLATIRDRASLIPGVLPMLSELRRRGLKVGSTTGYTQEMMAELRPRAAQLGYTPDAV
                     LTVSDVPAGRPAPWMALRCAEQLAAWPLSAVVKIGDTVADVEEGLNAGMWTVGFARCG
                     NEVGLDESELTALPQAAQDERIAQARKRLQQAGAHFVIDGPADLAAQLDVLELRRRRG
                     DRP"
     gene            complement(17482..19863)
                     /locus_tag="PKL17_09830"
     CDS             complement(17482..19863)
                     /locus_tag="PKL17_09830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNK45072.1"
                     /translation="MSETPVAAAEHAPPAPIAVANPPAAQAAAAEPAKRELGEADYGP
                     APRTFVEMGLSEDVAGALRDMGFSEPMPVQQRVFRPVIAGRDLIVQSRTGSGKTAAFG
                     IPFVQSVLDHDADVKNGCPQVLVLGPTRELALQVANEIGRIAARRKLSITAVYGGAPM
                     GRQVEALKAGAHLVSGTPGRVLDHLRRGTLNLSKVKALVLDEADEMLSMGFLEDITEI
                     LRRCPSDRQTLLFSATMPDEVLRLQNRYLRSPLNVQLSNEGISAAEIVHAYYMVTGMG
                     RMRDLLRVIEIERPDSAIIFCNTREETSAVAEFLRNHGHDAEPISSDLTQAERERVMK
                     RMKAKDLKFLCATDVAARGIDISDLPFVINYTFPESAEVYVHRTGRTGRAGKRGVAVS
                     LISPRELGNFYYLKLTYKIRPEERTLPTEAELQTLREGQYMDRLQKDLGHHNPGPEFR
                     SLLRRLLASESGEAILALLLEQHLQKPKAARPLHQAEHSARSHERRPREENRTPEERR
                     PRDENRTPEERRPRDEERRPRDEERRPRTDSAPAQSEARPESRPQATEVRPASPEPQP
                     RADVRPAAPEAEAPRTDFRPIGEERRPRDDRRGRERDDDRRGRDGRRDDRRDRDRRGP
                     RRPDEPAGAARAAETVPMPGSPATAALDIIHTDDGREFWEAWADSRAKTTHPDAKAIS
                     EPAPRADAPSSALVPQDGDGGERRPRRPLRDSRPPIAGEVRLYMNVGRRDNLDDAALV
                     AFVSSQGVGEIPMELHSSHAYLFVPEAQVDATIAALAGKPLGSRTVVCERARR"
     gene            complement(20391..21143)
                     /locus_tag="PKL17_09835"
     CDS             complement(20391..21143)
                     /locus_tag="PKL17_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45073.1"
                     /translation="MSLFAKGLEYGRLAFTEVGHRARTVEEDLKARREVDISGMVNGL
                     RARLAEQFPLEELPIDADLRSHALPLDVAKVSTWAYRSPKLRKIVLSHIVVGPTVLPA
                     VEGLALTVFPETEWDVPCFLADLMALPWWVSVNADVYGRDWQTQKVLSGLRSTFLRLG
                     SGQSPPFCARISSGDGLHAKLRPRQVEEGFAALTQALSAYLQLLQDPPPGRSDKDQQE
                     VFAAFHEHGPRMRLRRPFGDAFAERYSRLLFE"
     gene            complement(21234..>22109)
                     /locus_tag="PKL17_09840"
     CDS             complement(21234..>22109)
                     /locus_tag="PKL17_09840"
                     /inference="COORDINATES: protein motif:HMM:NF023945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNK45074.1"
                     /translation="DGWSTATLRALWEELKTAREKRTRSAAHEARWLNLTGFVLRPGF
                     GYALDDWRVKEAWRVFNEGLHHEKDDTCRLEWWILWRRISGGLSRNQQEEIWKRAAPL
                     VIPSLKRADKKAIAPHEMAEVFRVLAACERLEVKKKIELGDTVIALLEKKRSEFGLWS
                     IGRIGGRMPLYGPMDAVVSPSLADKWTQRLVRLVPGANDKDDERYQLAHAVTELCRLT
                     GDRVRDLQPAARQRVADWLREVVPGDDGARLSQMVLEVVPREEREERFAFGDSLPTGL
                     RLLGPGPDASPMGPL"
BASE COUNT         4269 a         7218 c         6629 g         3993 t
ORIGIN      
        1 agaagtggtt ttagttcatc gctgctcgac tgtgacattc caccgcccgc cgaagtcgaa
       61 ggctacgaga tcaaaaaacc gctcggacgg ggcgcgtatg ggacggtgtg gctggcggcg
      121 cagaaaaaca ccggcaagca ggttgccatc aagttttacg cccacgctgg cgggcttgat
      181 ttggtgctgc tccgtcgaga agtcgagtcg ctggcgtttc tttataccga acgagacgtg
      241 gtgcagctcc tggcggtcgg ctgggatcac gagccgccgt actacgtgat ggaatacctg
      301 ccacacggat cgctggagga gcggctgcgc aacggaccgc ttccactgac cgacgcggtg
      361 acgatcacga aagtggtggc gcgggcgctg caaaaagcgc acggtcgcgg cattttgcac
      421 tgcgatttga agcccgccaa cgtgctgttc gacggcaatg gcaagccacg gctgtgtgat
      481 tttggtcagt cgcgcctgtc gaccgatcac aagccgtcgc tcgggacgtt tttttatatg
      541 gcgcccgagc aggccgactt gctggcggtt cccgacgcac gctgggatgt gtacgcactc
      601 ggggcgatgc tgtacgcgat gctcgtcggg gcgccacctt atcgcgacgg accgcttcag
      661 ccgcttttgc ggcgggcgac gacgctgccc gagcggctgc gcgtctaccg cgagtttttg
      721 ttgtgcagca agccgcccga tattcagcat caccgacgag ggatcgacgc agcgctggtc
      781 aagatcgtgt cgcgctgtct ggccaccgac ccggcgcagc gctatcccaa cgtccaggcg
      841 gtgctgacgg cgctggatct gcgggcaacc cggcagcagc gacggccact ttggctgttg
      901 ggggcgcttg gtccggcgct gctgttggtg gtgacgtcgc tgattgcgct caaaggcgtg
      961 cgggtggcgg tgcagacgac cagcgtggcg gtgacgcagc gagcgcgcga atccagtcag
     1021 tttgccgcac gctttgtcgc cgagacggcg ggccgcgaaa tcgatcgacg ctggagcgtg
     1081 ctggaacaag cggcgctcga tccagcgctc cggcaggaac tgacgttgct gccgccagga
     1141 cacaccgcgc cgagcccgac gctacagcgc tttatcgaag cacagagccg tcaaaatgcc
     1201 gatctggcgg cggccagttg gtttgtgacg gcagtgtcgg gattccagct tgcgcgggca
     1261 ccgtacagcg acacaattgg ccgcaactgg gcgtttcgcg actactttca cgggctcggc
     1321 agagagctgc cacgcgatac gccgccgcag acgatcaggc ccattacgca gccgcatcga
     1381 tcggcagtgt ttccaagcca agcgacgcgg acgcggctgg tggcattttc ggtgccaatc
     1441 tggagcccga ccgagccccg caaagtggtc ggggtgctcg ggatgacggt cgaactgggc
     1501 ggttttggcg agctacgtcc cggtcgcggc gcgagcgagc gccaaattgc ggtgttggtc
     1561 gatggccgcg aagactggac cgggcaaaaa gggcttgtcc tacaacaccc gcagctgtcg
     1621 gcgctgctcg cacaaaagcg accgctgccg ccgtatcgac tggatgacca gcacatcaaa
     1681 accatcgaag cgctgcgccg ggcggctgcg gacggactgc cgctgccgct ggatctgctg
     1741 ttcgatgcgc actatcgcga cccagtgagc ggcagttacg gcggcgagtg gcttttgacg
     1801 atgcgtccgg tcttgtcgcg tcagcgggac accggctggg tcgtgcttgt gcaagagcgg
     1861 ctcaccgaca tcacccagcc gatcacagcg cttgggcaca gcttgctgcg aattggcatg
     1921 gtgggcctgc tgctgatggc ggtggtgtgg ctgcttttgt ggtggcgagt gagccgcagc
     1981 tttgttcgca cctaacgacc agcctgtagg ctgtatcccg acctgcgaaa aaatcgacgg
     2041 tttgtgcggc cttgactccg ccttgctttc ttttaggctg ttaacttatg acactgggtc
     2101 tggtttgcga cgcttgcgac acgcttagcc cgctgtcggc tacgctgtgc tcggtctgcg
     2161 gcgccgccct cggcagcaag tcggttcctg ggccgaccag tagcccgcgg agctgtccga
     2221 actgcgcatc ggaaatccct gggcaacacc gcttctgtgg tttctgcggc acacgcaccg
     2281 acggggttga tcttcgtcca gtctggcaca gcggcatcac caccaagacc cggctgaccc
     2341 tggttggcca agacggtcag gaaggtctgt cgttcccgct gaccgaaggc gagcatgttg
     2401 ccggacggct cgaaggcgcg atcatgtttc cggaagatcg gctgctttcg ccccgtcacg
     2461 ctacgttcct tttgcagagc ggcaagctgt gggtgcgcga cgacggatcg cacaatggcg
     2521 tgttcatcaa gctgcgaacg cccaagattg tgccgtcggg aaccgtgctg ctcatcggcg
     2581 aacagctttt gcgcatcgaa accgcgccgc cggacgtgct gccgtatccc gaccaggaag
     2641 gcacctactt ttactgctcg ccgcaccgtc cagcccggct cacgatggtg cagattctcg
     2701 tcggtggtga tatagggctc atctatcggt cgcgcggcga caccgtgacg atcgggcgag
     2761 agatgaacga tgtaaatttc cccgaagacc cgttcatctc gggacgccat gcgcagattg
     2821 tggcgctcga ggacggacgc tttcaggtca cggacctggg cagcaaaaac ggcacctacg
     2881 aaaaagtgcc gcggatggtc cagcttgcgt cgggcgatca gctgtttttg gggcagcagc
     2941 tcttgcgcgt ggaaatgggc tgattaaggc cgtgccttcc gtggagaaaa cctcgtggtg
     3001 aactgcagaa actgtggcaa ggccaaccct ccaatgcttc gcgcctgcca gtactgtggc
     3061 aatgcgctga gcgtcgaatc ggtgccgcgt ggagccttgc gcggacgcct ggtgctggtc
     3121 cgtcccgacg gcagtgaagg tggcgcacac ccgctgttcg agggcatcaa cgtcgtcggt
     3181 cgcggcagtg gcccactgtt cgacgccgat agcttcctgt cgccggtcca tctacgctgc
     3241 gagatcgcag gcggtcggtt tgtcgtccgt gatgccggca gtttaaatgg cgtattcctc
     3301 aagctcaccg aggaagagtc gctgtttgaa ggcgccatcc tgcgcatcgg tcaggaactt
     3361 ttgcgctacg acagcatgcg cccgccgaag cctcttcccg acggaaccga gatcatggga
     3421 tcgcccaacg ccgggtactg gggccgcctg acgatgatca tcggacgaac ccaggacggt
     3481 cgcatcatcg aaggcacgtc gtttccgctc gccggagaag ccgtcgtcat cggtcgcgag
     3541 cgcggcgaca tcctgttttc cgaagacggc tacgtatcgg gcacgcacgc gcgcttttcg
     3601 ttccgtcagg ggcggcctta cctgcaagac ctcgccagct cgaatggaac ctatctgcgc
     3661 attccggcgg aacgggctgt ggtgcccggc acgtttctct tgctcggaca gcagcttttc
     3721 cgtctgatgc ccgcatagtc gcaccgcttg gttagcgctg cggtttcccc ttgagcgatc
     3781 cgaccgtaaa gccagccgac tgtggctgcc gcaccgactg tggctgcgcc aacaccgcgg
     3841 gcgcggcgat gcgatcgttc gcgggtgcag gaggcgcggt ggacaggaca aaaaagtcac
     3901 gcttggtcgc gccgcagatc ggacagcacc acgaatcggg aatctgatca aacggcgtgc
     3961 ctggcgggat gcctccgtcg ggatcaccca actttggatc gtagacgtac ttgcactttc
     4021 ggcacttcca cgtcgtcaca acccagcgtt tgtagcacag cgaatgggga aacggcgcgc
     4081 ccgattgcaa gcaccgcggg gttacggcgt cgtgcgagcc agcgaacgaa tgtgggcgac
     4141 aagcccgtcg atctcttcgc gatttttggc aagcagaggg aacgcgggca tctgcgcgct
     4201 tttgcgcacc ccaggaccgc cccgcacgat cgcagcgacg agccgaccgt tcgatacgtt
     4261 cgactgccag accggatcgc cgaggtcttg gacccgcact tttttgttcg ccagcgcttc
     4321 gggaccgtca ccccagccgc tcaccccgtg gcagcgaaca cagtgctcgg cgaacagctt
     4381 ggccccttgg gctttggcgg cttccgacac cacgggcggc ggctcgatag gtggcagtcc
     4441 agtggtgcgc tgacagccga aaagcagcag ggcgcacagc gaagcggcct gggcattgcg
     4501 atgtgccttc atgacgatct gatctgtcct tgctgaatcg atttggcgat ggtgagcgcg
     4561 ccgaaaagtc cgatttcttc gccctgcacg acgtggatgg gaatcgacga caaaagaccc
     4621 gaaaagcggc ctttgcgagc gaagtgcgaa acaaacagtc ccgacgtaaa cagcggcaag
     4681 tttttggctg cgatgcctcc ggctagatag acgccgccgc gcgccagcga cttgagcgcc
     4741 aagttgccgg cttctgcccc gaagagcatg acaaagcgat ccatcacctg ctcacacagc
     4801 gggtccgtct tggcgagcgc atgacgcgaa atcaccctgg ctgggtcgtc acagcgggca
     4861 agctccgccg ccacctgcgg actttccgtt ccgatccgct catcgcgaaa aaatgcatac
     4921 agcgtcgcaa tgccagggcc gcagaccagt cgctcttggc ttacgtgatc gggatggcgg
     4981 cgctgtagat agcgccaaag ctggacttcc agatcatcgt gcggcgcaaa gtcggtgtgc
     5041 ccaccttccg tcggcaaaat caccggctca tcgccgtgaa acgcaatcag cgcttcgccg
     5101 agcccggttc ccgcccccag cactgcaatc ggtgaaccgg gttcagcgtg cgccaatgga
     5161 tggagcgaac aaagcgaaag ccccggaaaa ctgccgcccc gcgcgagcac cgacaccgcc
     5221 gctgccaccg cgtaaaagtc attcactagc cgcaccgacg caaaaccgaa gcgcgcagcg
     5281 atggatcgcc cgtcgattcg gtacggcaag ttggtgacat cgcaggtttc aaagccggcg
     5341 tgttttgcga ccggaccggc gacaccaagc gccatcacct ggggatgcaa gtccttcgca
     5401 gtcgccgcca gaaatgccgt caccatcgac tccaagtcgg ggtgctgttg acttgcgaaa
     5461 cgctgcttgt gcagcagcgt ccccgcgtcg tcaaacaaag cgagcagcgt ctttgtgcca
     5521 cccagatcgc ccgcgagcag cgtcatcttg gtttaggatt cccccgtctg acgaggcaag
     5581 cccatgctgt cgatggcacg ctcgctgaac ccttcgcgga acagaccttg ccacatcgcc
     5641 ggacggtaga catcgccggc aagcgcgctg gtgatcgcgg tgtacgtcgc ccgatgcgca
     5701 gccgatcgca cgagcagcgg acgcaggtgg cgcgcataaa actcgtgcac caggcctttg
     5761 aacagatccg cgccctgaag cgccgccgtc tgataacccg cgaatcgttc cgccgagcag
     5821 tcgcccgcca agagcgcttc gtggatcgcc ttggctgcgt acttgccgca cgtaaccgcc
     5881 atcagcacgc ccgtcgaaaa cagcggatca ataaatgccg ccgaatcacc gacgagaagg
     5941 aaccgatcgc catagaactg cttgcagcga tacgaccagt tgccggtggt atcgatcgga
     6001 cgaacccgcg tcgcgccacg cagcagttcc tgcatcaccg gggtggcttc gagcgcggcc
     6061 atgaacatct gctcggcgct atacccacgt cgcgccaccg taaagtgctt ctgcagcgtg
     6121 cagccaatcg acgtgacggt gcccttaaac gggataaacc accaccagcc accgtcgaac
     6181 agcaccatgg tgatgttgcc ttcgtctttg cccgtctgtc ggttgcagtt ttcgtagtgg
     6241 gtaaagcacg ccaggttggt cgtcacaaga tcgtcggaca ctttcagacc gaagcgcgtc
     6301 cccaagaacg tgtctcgtcc ggtcgcgtcg acgaacacct gcgcgcgcgg cacttcgatc
     6361 gatccatccg cgcgccgaat cgtcacatcg acgccgcttt caaaaaagcg cacgtcgcgc
     6421 acttcggttt cttccaacac ctcggcgcca agtgactcac tacgacgaag cagcaagtca
     6481 tcgaagtcgg cgcgcggcac ttcgtaggca aacggatgat cggggcgaat cgcgtcggaa
     6541 aagtagtacg tctgcgtaag acccgactcg ttgtgaatga agcgtgcgcc cggcttcatg
     6601 atgtagcgct gcttcatttc gtcggaaacg cccagctcgt cccagacttc gccgccgcag
     6661 ggaagcagcg actcgccgag gtgaaagcgc ggaaagcgct ctttttcgag caccagcacc
     6721 gaatgcccat gcttgcgcag gaagctagcc gtcgtcgagc cagcaggtcc gccgccagca
     6781 acgatcacat cgtaagagga ctttttcatg gtcatcacgg ttctccttat taggaatttt
     6841 gcatgcctct tatacacgct gtcgggccac gcgacgtacc catcactgcg ggcagtgccc
     6901 ggtcacgccg gctggacatg atccgggctc tgcgatgact ctgcggatgt tttggcacgg
     6961 tcgccatgga ggctcggatc gtacccacgc acgatcagaa gagaagtagg acattgtcgg
     7021 atcagaaact cttgttggat gccaaactgg cgcgcttcaa gtccccagtc acgcccgaca
     7081 cccaccagca ccaggtcata cggcgaggcc gtctcgctgc ggatttcagc cagtgcagcc
     7141 tcggcgggct cggcggccag gacccgcttc accgtcacgt tcgcggcatc ggtaccaaag
     7201 acttcgcgca gcagctgatc gacgtcgggc tgactcggcg cggtttggtc atcatcgacg
     7261 acgtgtagca gcgtaatcga cgcgcccgcc tgatccaaaa gccgtcgcgc gagctgaagc
     7321 gccgcccgat cctgcgccgt gcgatgaaac ggcaccagca cccgcgtgat gttctgcaga
     7381 ccgcgatcca ccaacacccc gacatcggcc cgcgcatctt ccagcacttc accgacggtg
     7441 ccagacagcg ccgcacgatt aaacagcgga cgatgccagc caagcagcac caagttggcg
     7501 cccttcacct cgctgacgtt ggcaatgtca cgcgcaagat gcgacgacac aaacgcgatc
     7561 gggcgcactt ttagctgcgc ttcctcggcc agcgccagca ccggacttag cacctgactg
     7621 gccgcctgcg ggtcttgccg atcggcgacg tagtgtgacg gtcgttccgg cacccgctgc
     7681 agcttgagcg catagatccg gctgcgctcg cgactgcgca acgtcagcgc cgacgaaagc
     7741 ttgatcatcc ccggcaccgt cgctgggtac gacacgcaga gcaacagaca gaacgcatcg
     7801 acagcccgtg gctcggccag ttcagcagaa atcaagctct gcggataaat cagcttgagc
     7861 agtggcgtcg tcgacaccgt cgaaaccagc gccaccagca ccatgatcgt gaacatcgtc
     7921 ggcgatatca cccccagatc gagcccgatg ttaagcacca caagctccat cagcccctgg
     7981 gtgttcatca tcacgccgac cgccgccgac tcgcgccacg acagacctga cacgcgcgcc
     8041 gccagcgtgc atgcgccaaa ctttcccacc accgccagcg acagaaacag cgcaaacatc
     8101 acccacaaat gggcctgcga aagcaggttc acttgcgtgc gcagccccga aaacgcaaaa
     8161 aacagcggca gcaaaaacac caccgcgaag tcttccagcc ggtcggccag cttcgccgcg
     8221 aaaccgccct ctttgggaag caaaatgccg aacaaaaacg cgccgaacag ggcgtgaatg
     8281 ccaatcagct cggtcgtaaa gctcgacaga aacagcagca aaaacaccgc ggcgaccagg
     8341 ttttgcgaca gaccttcgcg acctgcacct cgctgtccga gacgacgcaa aagcggtcgc
     8401 acgacaaacc acatcaccgc gacgtacacc aaagtccaca gcgtcggctt tagcgacgat
     8461 gacataccag ccgatcgggc cgtcgcgacc acaaacgcca aaagacacca cgccagcacg
     8521 tcatttaccg ctgcgcaggt cagcgccacc gtgccgactt tcgagtgcag catctgcgtc
     8581 gccgacaaaa tccgcgccag caccggtacc gccgtaatcg acagtgccgt ccccaaaaac
     8641 agcgcaaacg acagcggacg cacatctggc gacgacagcc ggtcgtaaag cagcgtcgct
     8701 gtcccgccgc ctatcgaaaa tggcaccgcc atgcccgcga tgctgatgag cagcgacgcc
     8761 cggccatgct ttttcaccat cttggtgtca agctccagcc cgacgaggaa catgaacagc
     8821 accagtccga cttgcgcaaa aatctgcaaa agcccgagcg aatttttcgg aaacagcgcc
     8881 gcactcacgc ccggccacag ccagccgagc agcgatggac ccagcacaat cccggtcagg
     8941 atttccgaaa tcaccatcgg cagaccgaca aagcgcacga cgagtccaag cccgcgcgcc
     9001 accgcgatga tcagcacggc ttgcgcgata aacagcgcaa gcgggctatg aaaaacggac
     9061 tcaaacatcg acgaaacacc ttgatacacg ccttccccat gccggtcaac gccgacggaa
     9121 aatcgtagac agcaagcgca caggcccaaa gcccgccacg gccacggtta agaccggttt
     9181 gtgacttgac aaggcacggg cagccggcaa aaggtgtggc ccgcatgcat cgttttgtca
     9241 gtcggtcttt gcgaaaaaag agtctggttt tcacgctcgc accgctgctt gcttcggggt
     9301 gtctgccgga cccagacatc aaggtgccgg acttcaaacc gtcgggaatc gtcgatctgg
     9361 gcaaagcgcc gctcgacatg acaacgctgc ccgatgccgg tcgcagccgc tttgttcccg
     9421 atggcgccgt tcccaccggc cagacgctgc gcgcgattta cgtcgatgcg ggcggccaaa
     9481 tcttcgtcgc tggacatggc ggtacgatct tgcgacgcag cggcacggtc tggaccgtcg
     9541 aacaagcgat cgacggagca gccccaatca ccagcaactt gtacggtctt gccggcagtg
     9601 gcagcgagct ttacgccgtc ggtgaagccg gtgtggtggt aaaacgcagc ggcgacaagt
     9661 ggctgcgcga aggcaaaaca ctggcggtca ctgcatcgct tttcgcgacg acgatcacct
     9721 cggcgggcga tgtctatgca atcggcgact tgggcaccgt gctgcgcaaa ccaatgggcg
     9781 gcacctggac caaagaagcc gtcagcgccg gcttggccac cgccgagttt cgcggcgtgg
     9841 ccgaaaccaa aaacgacgag ctaaccgccg tcggactggg tggtgtcatc gcccagcgca
     9901 gcggctccgc gtggaacagc gacgcacttg ccatcgacag cgccgaccgc gtcaactttt
     9961 acggggtcgc caagaccagc gaagccgtgt tcattgccgg cgattccaac cgagtactcc
    10021 gtcgagatgc cgacaagtgg cggcgcgaac cgaccggcaa tctcaccggc ccaagtgcaa
    10081 acctcttcgc gctttctgcc agcggccaag acctgatcgt cgctggggcc gccggactta
    10141 tcgaacgccg cagtgcccaa agcaagacct ggtccatcga agacagcgga accaccgtca
    10201 tgctcagcgc tctgtccaac gtatcgccgc tgcgtgccgt cggtgcgcaa ggaaccatgg
    10261 tggtgcaaaa gtgatccatc ccgtcattgc catcgacggt cccgccggag ccggaaaatc
    10321 gaccgtggcg cgtgaactgg cccgtcgttt gggttttttc cttctcgata ccggcgcgat
    10381 ctatcgcagc cttgccctgt ttgcgcagca aaacggcatc tcgtgggccg acggtgcaaa
    10441 actcggtacg ctggccgaaa cgctgcccat ccgctttggt cgcggcagtg aagacggcgc
    10501 ggtgtttctc ggggaacagg tcgttacgac cgcgattcgc agccctgaag tctcgctcgg
    10561 tgcatcgcaa gtatcggccc atccggaagt ccgggcgatg ctgctcgggc tccagcgcca
    10621 gcttgcgcgc agcggtccct gcgtcgtcga aggtcgcgac attggcaccg tggtgctgcc
    10681 ggacgctccg ctcaaaatct ttcttacggc gtcaccagaa gtccgggcgc agcgacgaca
    10741 aaaagaactg gaagcgcgcg gggtcaaagc atctgccgaa gcgacccgtc gcgaacaaga
    10801 agagcgagac cgccgcgatc tcacccggac gaccgctccg ctcaagtgtg cggacgatgc
    10861 ggtgctgttt gacacgagcg aacactcgct cgaacaagtg ctagacgcgc tggtttccgt
    10921 cgcgcacgaa cgactcaagc tgtaacgctg cgatgggccc caaaaccggg gccgccacat
    10981 cgtcaaaaag tcacgcgggg gaagtcagcg atcaggaagt gggcgaatgc ctacgaggat
    11041 gggtagcgaa aaatggtcaa tccactgcga ccactcgcgg ccagtcgctg gcagagctga
    11101 tcgatttctg agtcgaagtc gaccaagatg ggccctggtc ccctgccctt ttctgcctgg
    11161 gcaactgcga cccactgacc tgcgagctga aattgcggac cgtcactcgt ttcggtttgc
    11221 ggcatggtgc aaaaggcaat ggcggacagc gccgccgtaa gaaccttggt tacatccata
    11281 atccgcatgc ggtcaagctg caactccgcg aatttgtaga atttttggcg cgaccgcaca
    11341 agcggcgtac tttcgggatt acttggtggg cgtcgcgggg agcggtttgc cttccacgtc
    11401 gcgtcgctgc acatcggcgg gcgaaagccc cagcgcttgc ggcagcttcg gcaacagctt
    11461 ttgtacgtca ccgacgatta cgatcgtggc gtcttcagga tggagccggc tgcgcccgac
    11521 ttcttgcagc ttcgccgctg tcaccgtctg cgctccactc accagcttcg caaactcgtc
    11581 cattggcagg cggttgcgcg caaacgtcgc gtaaagctgc gccagccccg cggtgcgctc
    11641 gctctgcatg accagtcgct ggatcgcacc ggcccggctg cgctgaagtt cctctgccga
    11701 acagacctca tcgcgcatcc gcgtcacttc attgcgcagc tcgacggtcg actcggtggt
    11761 tacatcgctg cgcaccgcgg ccatcgacac ccagtaaccc agcccatcca ttcgcaccac
    11821 ctgcgagcgc gctccgtagg tgtagccatg tttttcgcgc aggttttggt tgagtcgact
    11881 ggtgaacatt ccgcccagaa gcacgttcgc catgtccatc gcgggtcgct cgggatcgag
    11941 cgcaccgggc accaagttgg caaaccgcag ctcggcctgg ggagcccctg gacgatcgac
    12001 aaaaatcagc ccacgctgcg gctttggcgg ggtccgcggc ggtctcacaa gcgacggcga
    12061 gcccgctggg aatggcagcc gcccgagcac ccccagcaag tgatccgcca ccgcctgcga
    12121 cggttcaaag tcacccgcga tcacaatctt ccagtttttc gacggataga agctctggtg
    12181 aaacgccttg aggtccgaca ccgagagctt ttgcaaagac gcggcagtac cttgcggcga
    12241 acgaccgtag ggctcactcg caccaaacag gctggcgcgc agcacgcgat cggccacctg
    12301 cggcggctcg tcgtttttct gctccagcgc ggccaggcgc tcgcctcgca cgcgcgtcca
    12361 ttccttgtca tcgaaccgag gccgcagcag ggcttcataa aacagcggcg ccgcttccgc
    12421 ataggtctgc gacagcacgc ccatcgaaag cagcgtcgta tccgacgata cttgggtctt
    12481 ccacgtcgcg cccgccgctt gcatcgccgc cgcccagtcc gtccccgcgc gttgaccgct
    12541 gccctcgctg agcatcgccg ccaccgattc cgccagaccc gacttcgccg ccaccgcatc
    12601 cgcttcaccc gtcggaatcg ccaccgccgt ttcgataagc ggcgtccccg aacgctgcac
    12661 cagcgtcacg tcgacgccat gcacacggaa gtgcgcactt gtggggggtg tcggcggacg
    12721 cagcgccagg gacttcggct gcttcgacaa atcgaaaggt gcccgcatgg gcccaggaag
    12781 cggctgtggc ttgggcgcct tcccacctgg ataaaacggc tgcggcttgg gcttgctcgc
    12841 ttcagccacc ggcggggcag gcggcggacc tttgcgcggc accacctgaa tttgcagacg
    12901 acgaccgcga tccccgacac cgagatccag cgcgcgcttt tgcagcgact ggggtgtcgc
    12961 catccggaac ttcccgacga gctgcggcag tccattttga tcgccggacc acgcaaaaag
    13021 ctgctgaagc cacagcgact gcccaagtgg cgtctcgatg tcacgcgcaa gctccgtcag
    13081 ccgaaggttg cgggcccgcg cgatttcctc tgccgttggc ggagcactga caaacgtcgc
    13141 cagctctttt tcaaccgcag cggtaagctg ctcggtcgtc acgccttgct gcgccgtcac
    13201 cgagagcgac atcaccgaac caagctgctg cggatcgact tctgcttcga ccgcctgtgc
    13261 cagcttctgc tcgacgacca gcttgcgata gagcaagctc gctttgcctt tccccagcaa
    13321 gtcagcgacg agcactgaat cgggaaaatc aggcgtccca acgggcggcg catgccacag
    13381 cacgtacaca cgcggcagcg tcacttgatc ctcgatcgtg accgattgct cgacgggaac
    13441 aaccatctcg ggcacgccat tttgcaacga cggcgccgcc ggcttgggcc gcttgggcat
    13501 ccagccaaag taggtgctga tcagcttgcg cgcttcctcg ggattgaaat caccgacgac
    13561 ggccagcgtt gcattcgacg gcgtatagta cttatagaaa aactgtttta cgtcgctggt
    13621 gctcgccgcg atcaggtctt cgtgtgaacc aatgatcggc catgaatacg gatgccccgc
    13681 cggaaacatc gccgatgaga cgcgcagctc cgccatgccg tagggtcgat tttcatacga
    13741 ttgccgacgc tcgtttcgca ccacttcgcg ctggagcgag actttttcgt cggtcatcgt
    13801 ctctggcagg ttggtcaaac gatcggcttc gagccacaac agcgtcggca aaagatgact
    13861 tgggccaaag tcgaaataga acgtccggtc cgtgctcgtc gtcgcgttgt tgccgccgcc
    13921 ttcagactcc atcagcacgt cgaagcggtt gtccggcaca ttcttcgtcc ccatgaacat
    13981 caagtgttca tacaggtggg caaagcccgt ccgccccgga gcctcgtcgc gagaaccaac
    14041 ggaaaaccac agcgacacaa ccacgcgcgg caccgacgga tcacgacgca aaagcaccgt
    14101 cattccactc ggcaaggtga agcgctccgc cgtcagcgaa aacggatcac gactcgctgg
    14161 attcgccgcc ggggtagccg cagcagccgg tgggcgcaat cccgtcgctg caggcggggc
    14221 tgcgaaagtc tgtgcgcttg ccacaagcag caccgctgcc gatacagcag aaggaaagac
    14281 gcgacgcagc gccgacatca ggcaagctcc atttccagac tgggccgtgg gtcggtcaga
    14341 ggccgtggcc gatacagcag caggttgcga ccgatcacct gcacgagcgc tgcgcgacag
    14401 tctttggcca gctgctccgc cagttcgtgc cgatcgcccg gtgccgtctc gagcagcttg
    14461 atcttgatca gctcatgttg ctctagcacc gtcgccaccg ccgcaagcag accttcgctt
    14521 atgccgtctt tgccgagctg cacgacggga ttcagatgat gcccaaggcc acgcagcacc
    14581 gtccgctgcc gattggtcag ctcgggggct ccagaaggac tgagcttggt aacacgcttg
    14641 gttttgggac gagcagagga acgttcggcg ctgagctttg ccatgtcggt gaccctaaca
    14701 aattgctgac tgacggtggg gaaaacgtat gaacgtgtcc aaatatgccg caaggtcgca
    14761 aaaaaattgg ctcgcgactt tacacgacgg acttgagcga aaatggcgat gagggggcgc
    14821 gatgaaaaaa accacattcg acgtgctgcg cgacggcaaa gagggaccgc ttttgtttgc
    14881 gtgtatgcag cggcccgacg accgcgaagc ccggctgcgc tatctccagt ttctgcaagc
    14941 gacgggcgat ccgcgggccg aacctctgcg tctccaggtg cagctttcga cgctgacgga
    15001 atccggtagt ccgcagacgg cacaaatcga ggaaacccgc cgtcagctac gaaagcttgt
    15061 cccgacgctg gatcgtgttt ggtgggctgc tttggcgcgt tcaccagaga cgtttcactg
    15121 tggccaagcc aggtcggctc cgtcgctcca cgttcgcttt cgctttgagt gtccgcaaaa
    15181 ctgggatttg ctgacggaaa cgggcgatcc gagccaacgc ttctgcacag tctgcgcgca
    15241 gacggtccat tttgccgaca ccgtctcgaa agccgaacac ctggcgcgac aaggggcctg
    15301 tatcgcggtt ccgagtgcgc tcgtcgacaa aaccgtccgc aaagtcaccg agctggcggt
    15361 cggacgcccc gactggaaaa aaatgtgggc cgatcggatt ttcggtccaa tctccgacga
    15421 ctaactgccg cgatcgccgg cttggattgg ccgcagcgca tgccttgggc tactgtgcgc
    15481 agcgctcatg cagaccatcg ccccagcttc ttcgtctttg ccccaacggc tgtttacgcc
    15541 cggtccactc tcaaccacag cgacggtcaa agcggccatg ctcgtcgact tgggatcgcg
    15601 cgatccgttt tttttgcgca ccgtcgcaga gctgcgcacc gagctgctcg ccatcgccca
    15661 ggtgtccgaa cccgagtttg ccgccgtccc catccaaggc agcggcacct ttgcggtcga
    15721 agcaacgctc ggcagcgccg tcggaaaaac cggcgggctg ctgattctgt ccaacggtgc
    15781 ctacggcgat cgactcgcgg acatcgcccg cagcgtcggc atccctcacg tcgttcaccg
    15841 cttcgccgag accgaaccgc ttgatcccca cgtcattgcg acggcccagc gcaaggcccc
    15901 tcatctgagc cacttggcgt ttgtgcactg cgagacctcg acgggacaaa tcaacaacat
    15961 cgatgaggta agcatcacag ctcgcgccgc cggcctgtct gtcatctgcg atgcaatgtc
    16021 gagctttggc gccattgcga cgccgctcga ccgactgccg attgattttt tggtcacgtc
    16081 ccccaacaag tgtctgcaag gcgtgccagg ctgcggtctt gtgctgctgc gtcgctcagc
    16141 gctgcgcaaa ggccaggcgc acagcatgag cctcgatctg tgggcgcagc tcgacgggct
    16201 ggatcgcagc ggtcagtttc gcttcacgcc accgacgcat gtactcttgg cgctgcgtca
    16261 ggcactgcgc gagctggcgg aagaaggggg ccaggagcaa cgacgggccc gctaccaagc
    16321 caactcgaac ttgcttgccg acggcatggc caagctggga tttcgcacgc tgctgcccaa
    16381 agcggcgcag agcccgatca tcagcgcgtt ttactatccc gacgacgcgc gctttgactt
    16441 tgcagcgttt tatcgctcgc tcggggaacg cggcctggtc atctatccgg gcaaggtcag
    16501 ccacgcggcc tgtttccgca tcggcaccat cggcgatctt tatcccgacg acattcgcga
    16561 acttctgacg gcgattcctt tggtcctttc gacgcaggga atttctgtgc cgctttccgc
    16621 cactccctcg gagccacgct catgttagac gcccacagcc cagacagccg cgtgccattt
    16681 gtccccctca aagccgtcat tttcgactgg gcgggaacca tctgtgactt cggcagcctc
    16741 gcgccggtcc aagcgctgca gtcgctgttc gctcgccacg gtgtccccat cacctgtgcc
    16801 caagcgcggg gtccgatggg tcgagccaag cgcgatcaca tcaccgacat cctggcgatt
    16861 cccgacgtgg cgcgtcgatt ctcagaagtg catggcaagc cgccgcagag cgccgacatc
    16921 gatcgcctct ttgccgactt tttgcccatt cagcttgcga ccattcggga tcgtgcgtcg
    16981 ctgattcccg gtgtactgcc catgctttca gagctgcgac gacgcggtct caaagttggc
    17041 tcgacgacgg gatataccca ggaaatgatg gcagagctgc gacccagagc ggcccagctt
    17101 ggctacacgc ccgatgcggt gctgaccgtt tcggacgtac cggcgggtcg tccggcgccg
    17161 tggatggcgc tgcgctgcgc cgaacagctt gccgcgtggc cgctgtccgc agtggtaaaa
    17221 attggtgaca ccgtcgcaga tgtcgaagaa ggactgaatg cggggatgtg gaccgttggc
    17281 tttgcgcgct gcggcaacga agtcggactc gacgaaagcg agctgaccgc gctaccgcaa
    17341 gcagcgcagg acgagcggat cgcccaggct cgcaagcggc ttcagcaagc cggagcgcat
    17401 tttgtgatcg atgggcccgc ggacttggcg gctcagctcg atgtgctgga actgcgccgc
    17461 cgtcgcggag atcgtccgta gttaccgacg agcgcgctcg cagaccacag ttcgagagcc
    17521 cagcggcttg cccgcgagtg cagcaatcgt ggcatccacc tgggcctcgg gtacaaacag
    17581 gtaggcgtgc gagctgtgta gctccatcgg gatttccccg acgccctgcg acgacacaaa
    17641 cgccaccagc gccgcatcat ccaggttgtc gcgacgaccg acgttcatgt acagccgcac
    17701 ttccccggca atcggcggac ggctgtcgcg cagaggccga cgaggacgac gctcgccacc
    17761 gtcgccatcc tgcggaacca gcgccgacga cggtgcatca gcgcgcggcg ccggctcact
    17821 tatcgctttg gcatcgggat gggtcgtctt ggcccgcgag tccgcccacg cttcccaaaa
    17881 ttcacggcca tcgtcggtgt gaatgatatc gagcgccgct gttgctgggc tgccaggcat
    17941 cggaaccgtc tctgcggcgc gggcagcacc ggctggttcg tcgggacgac gcggaccccg
    18001 acggtcacgg tcacgccgat catcacgacg gccatcgcgg ccccgtcggt catcgtcccg
    18061 ctcacggcct cttcggtcgt cacgaggacg cctctcttca cctatgggcc gaaaatcggt
    18121 ccgcggcgct tccgcttcag gcgctgccgg tcgtacatca gcacgcggct gcggttccgg
    18181 tgatgcggga cggacctcgg tggcttgcgg tcgactttct ggtcgtgctt ccgactgggc
    18241 cggcgcactg tcggtgcgcg gacggcgttc ttcgtcacgc ggacggcgtt cttcgtcacg
    18301 cggtcgccgc tcttccggcg ttcggttttc atcacgcggt cgccgctctt ccggcgttcg
    18361 gttttcttcg cgaggccgcc gctcatgact gcgcgccgaa tgttccgctt ggtgcaaagg
    18421 tcgcgccgcc ttgggttttt gcaggtgctg ttcgagcagc agcgccaaga tcgcctcgcc
    18481 cgactcactc gcgagcagcc gccgcaaaag cgaccggaac tctggccccg gattgtggtg
    18541 cccgagatcc ttttgcagcc ggtccatgta ctggccttcg cgcagcgtct gaagctcggc
    18601 ttccgtcggc agcgtccgct cctcgggccg aattttgtag gtcagcttga ggtagtagaa
    18661 gttgccgagt tcccgcgggc tgatgagcga caccgcgacg ccacgcttgc ccgcccgtcc
    18721 ggtgcgtccg gtgcgatgga cgtagacctc ggcggattcg gggaaggtgt aattgatgac
    18781 aaacggcagg tccgagatgt cgattccgcg cgccgcgacg tcggtggcac aaaggaactt
    18841 gaggtctttg gccttcatcc gcttcatcac ccgctcgcgc tcggcctggg tgagatcgga
    18901 cgaaatcggc tcggcgtcgt ggccgtggtt gcgcagaaac tctgcgacgg cggatgtctc
    18961 ttcgcgggtg ttgcagaaaa tgatcgcgct gtcgggccgc tcgatttcga tgacgcgcag
    19021 aaggtcacgc atccggccca tgcccgtcac catgtagtag gcatggacga tctccgccgc
    19081 cgaaatcccc tcgttcgaga gctggacgtt gagcggtgag cgcagatagc ggttctgaag
    19141 ccgcagcact tcgtcgggca tcgtcgccga aaacagcagc gtctgtcggt cagatggaca
    19201 gcgacgcaga atttcggtga tgtcttcgag gaagcccatc gagagcatct cgtcggcttc
    19261 atcaagaacg agcgctttca ctttcgacag gttcagcgtg ccgcgtcgca ggtgatcgag
    19321 cacgcgaccc ggcgttcccg acaccaggtg cgcgccggcc ttgagcgctt cgacctgacg
    19381 acccatcggc gcaccgccgt agaccgcggt aatcgacagc ttgcgacggg cagcaatgcg
    19441 accaatctcg tttgcaactt gcaacgcgag ctcgcgcgtg ggcccgagca ccagcacttg
    19501 cggacagccg tttttgacat cggcgtcgtg atccagcacc gactgcacaa acggaatgcc
    19561 gaatgcggcg gtcttgcccg agcccgtgcg cgactgaacg atcaggtcgc gaccggcaat
    19621 gaccggacgg aagacccgct gctgcactgg catcggctcg gaaaagccca tgtcacgcag
    19681 cgcgccggcc acgtcttctg aaagacccat ttcaacgaac gtgcgcggcg ccggaccgta
    19741 gtccgcttcg ccaagctctc gcttggccgg ttcagccgca gccgcttgcg ctgccggtgg
    19801 attcgccacc gcaatcgggg ccggaggagc gtgctctgcc gccgcaaccg gggtctcgct
    19861 caccgggaca gaatcagcta ggggcgcagg ggcgggggat ggttgagcgg tggcggcttt
    19921 ggcctccgct gcgccagcga gcagctgtgt gccgtcgctg ggcgtgccgt gatcttgcat
    19981 cgaaacctct ttttggggtc gttcctgtgc agcgccgggc tcctttggcc aggcagatca
    20041 tgcgacagca gacggtgcac cctcgtcagg tggacggtcc gcgcacgcaa ggcgcacaga
    20101 acgacaaggt actgggggga gtcgaaacca accgatgtcg accgtcgaca cggtggcgac
    20161 tccacgttga aagggaaagg cacaagccca tctttgcggg acagctcgac cacgacggcg
    20221 cctcggtgcg tcgcaggctg gctaacagtc aagtccaagg cgctccgact tgcggcggct
    20281 tggcatgtaa cttgacggct cgaaagggga aatagctgca aaggagggct tcggaaattc
    20341 gcttggtttt cgaaacgcta gcgctgccct ctactgctgt caaggagcgg ctattcaaac
    20401 agcaagcggc tgtaacgctc ggcgaatgcg tctccaaatg gacgacgcag ccgcatccgt
    20461 ggcccgtgtt cgtgaaaggc cgcaaacacc tcttgctggt ctttgtccga tcggcccggc
    20521 ggcggatctt gcaaaagctg caaataggcc gacaacgctt gcgtcagggc ggcaaagcct
    20581 tcctcaactt ggcgcggacg cagcttggcg tgcagcccat cccccgacga aatccgcgca
    20641 caaaacggcg gactttgacc actgccgagc cgcaaaaacg tcgaccgcaa ccccgacagc
    20701 actttctggg tctgccagtc acgcccgtag acatcggcgt tcaccgacac ccaccacgga
    20761 agcgccatca agtccgcaag gaagcagggc acatcccact cggtttccgg aaacaccgtc
    20821 agcgccagcc cctcgaccgc gggaagcacg gtcggaccga cgacgatgtg gctcagcacg
    20881 atcttgcgaa gcttggggct gcgatacgcc cacgtcgaca ctttcgccac atccagcggc
    20941 agcgcgtggc tgcgcaagtc ggcgtcaatc ggcagttctt cgagaggaaa ctgctccgca
    21001 agccgcgcgc gcagcccgtt caccatcccc gaaatatcga cttcgcgtcg ggctttcagg
    21061 tcttcttcga ccgtgcgcgc ccgatgaccg acttccgtaa aggccaaccg gccatactcc
    21121 agccccttcg caaacaagct catggccggt caggctagca caaaaatgcg cgccggctag
    21181 accaggcaat ccgtcgagcg tttagacctg tccacagccc cccgcggcga cagttacagc
    21241 ggacccatcg gcgacgcatc gggcccaggc ccaagcagcc gcagacccgt cggcagcgaa
    21301 tcgccaaacg caaagcgttc ttctcgctcc tcacgcggga ccacttccag caccatctgc
    21361 gaaagccgag ccccgtcgtc gcccggcaca acttcacgca gccaatcggc cacccgctgc
    21421 cgcgccgccg gttgcagatc gcgcacccgg tcgcccgtga gccgacacag ctccgtcacc
    21481 gcatgcgcaa gctgatagcg ttcgtcgtct ttgtcgtttg cccccggcac aagccgcacc
    21541 agccgctgtg tccacttgtc cgccaggctc ggcgacacca ccgcatccat cggtccatac
    21601 agcggcatcc gtccgccaat ccgaccgatc gaccacaggc caaactcgct gcgctttttt
    21661 tcaagcagcg cgatcaccgt gtcacccagc tcgatcttct ttttcacttc gagccgttca
    21721 cacgccgcga gcacacgaaa cacttcggcc atttcatgcg gcgcgatcgc ttttttgtcc
    21781 gcccgtttca gcgacggaat gaccagcggt gccgcccgct tccaaatctc ttcctgctgg
    21841 ttgcgcgaaa gcccgccgga aatccgtcgc cacagaatcc accactcgag ccgacaggta
    21901 tcgtcctttt cgtggtgaag tccttcgtta aagacccgcc acgcttcctt gacccgccag
    21961 tcgtcgagcg catagccgaa accaggccgc agcacaaagc cggtcaggtt cagccagcgt
    22021 gcctcatgcg cggcgctgcg cgtccttttt tcgcgcgccg tcttcagctc ctcccacagc
    22081 gcccgcagcg tcgcggtcga ccagccatc
//
LOCUS       DAORGN010000071        21675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_953_length_21675_cov_6.426411, whole genome shotgun sequence.
ACCESSION   DAORGN010000071 DAORGN010000000
VERSION     DAORGN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 21675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21675
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_953_length_21675_cov_6.426411"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKL17_09845"
     CDS             complement(<1..452)
                     /locus_tag="PKL17_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNK45075.1"
                     /translation="MARCKNCGKANRDGALFCQDCGRSIDGSAYGPGGGVAAVAATAA
                     SASIPIAHDDEPLPIGRSAAAQVVSTPESPAPGSERSPTKEAGLGGTSASADSPCESC
                     GTTNPISASFCRSCGQNLNLKQRPQTKIACSHCGHPTPAGFMYCQHCG"
     gene            690..1448
                     /locus_tag="PKL17_09850"
     CDS             690..1448
                     /locus_tag="PKL17_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45076.1"
                     /translation="MSRSTPAPRSIPSAENRTIAKSRRSLSHVANLVVRRWLVFGLGL
                     WAMLFGLAGCMGPVAKASFAKRLGSGEAGNLLGPFDGLVIDQSTSNPLASALVVGTWA
                     FQETGGLAVPESSYTVTAVTGSDGSYSLPSLPAGRQFAGLLRRFTLVVYKAGFVGYRS
                     DLRFEDRSPRHNFVQRGNVVRLDRFPANESHVRHLVFLGSGAALRGAAQAEIIAASLE
                     LQDAAPKLAAEESAPSDTTAPKPATGDSAVKEGS"
     gene            1450..2934
                     /locus_tag="PKL17_09855"
     CDS             1450..2934
                     /locus_tag="PKL17_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45077.1"
                     /translation="MRRQQVRRFLGAFALALLSQSPGPSLAYEGATTLAGLTEQAALK
                     SRLHRRMLERFALSLGLFEPLRLDPALIGEDKARSLLIRLLALDAGQGYAPEPLPRKA
                     GQSFAPLRLHVLGWLAAGAVLEQSPAQRVRHHFADCKTGGGLSRPAGMTAGAARTEAV
                     SQGLSSVRQLLAGAAMDGTGQSAMDWIESPENELGLATFLDAYERSVVAETAEARESS
                     LVEALLAAGAMLSVIEQAGDPAYVHSDLSAVLAGDYSHYVAEQFGRAGIPEPDAKLDV
                     PSPQRLRDLIFDGKGNGLAERTGRFLSSGMISSSVLPAGTKLVGPGGYLSTPAAKHWL
                     LWGQPPRLSDGEWPAPRFVLDERCFADYATALLPEVGRYAQAGLDFLLRGDLRIAMQG
                     ETQLQIKLLDEPLGSGVVTLIGEKSSGERQVLKTLSTLPSRPGVLGLVPLGDVSIKDY
                     ARLVVLWKGRDRQGQSLVSSAQVALHKTDAVAPAAATTTEAASE"
     gene            complement(2937..5159)
                     /locus_tag="PKL17_09860"
     CDS             complement(2937..5159)
                     /locus_tag="PKL17_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNK45078.1"
                     /translation="MIGTPTQKRLRCGRGTLAGLFFGLCQLLLTASAHAQAPAEAPHP
                     AASYPVFDLLANRTLAHIEQRGGLVIPAGSAGFARYAHFGRPTPTWKLRAEVDGKKVA
                     LPTTAAKLQVPLHSGQATNQVVYLQLKSPIKSSLKIAVGSKSSTAVPLAEGWQLVTVS
                     LPPDSLHAGENTLSLTFAQSGSFAIGGGASHKAAAAVEWIQVGGTPPASAVTMPKLSD
                     GNRLLIPGGGALHYYAFVPKGGALALKGDAGACALKLTVEGPQPEKRGAPQAVSLDGS
                     PVALGSHGGEIRRLGLRAEGSCDKIALDKAQLLAAGSAPQLRKTEKPKNVVFWLSDDT
                     RADKFKLWNKSSRVETPVLDEFSKRATRFAVTYSQGNESRVSHASLFTGLYPSQHKFI
                     SDKAVLPDSFVIIPEVMKQAGLYTIAHIANGYITKRWGFGDGWDLLKNHIHEGGGLKS
                     AELVGDAANFIEKGPGKTKPFFLYLGTIDAHVSWRAYEPWISKYDPNPYSGPFVKGCM
                     DPQLDKIVAGQLTVTARDRQRVIALYDCDISYNDHHFGKLLELLKKTGHENDTMIIYT
                     SDHGEEFWDHGRIGHGQSLRQELIHIPLWIYYPPLFPGGKVVEEGAELVDLLPTLADA
                     FGMPIPKDVQGESLIPIAQGVSGGYPRPAIASQYELAHVMRLGRYKLWVGGSGQVKLY
                     DGQDDPGEQKELNQEQPLALRFVTDAMGLWMAYQDKWKKTRWGAASNHRPEFAADLEK
                     "
     gene            5421..6797
                     /locus_tag="PKL17_09865"
     CDS             5421..6797
                     /locus_tag="PKL17_09865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNK45079.1"
                     /translation="MASKHPRGLYVLFFTEMWERFGFYLMLALFTLYLTEHLRMTKEE
                     ASDLYGSYMFFVYLTPFFGGVLADRLLGYTRAILMGAALLGAGYLVFSIQNQTAFYAA
                     LFLMVIGNGLFKPNISTLVGNLYPTGDPRRDSAFSIFYMGINLGALFAGPVGEFMRTK
                     FGWPLAFATAGIGMVFSFLIFAALRNWVTLGDTRSSVAAVLDVPLPAEYEDRPDPPEV
                     ERQRIIALTIMCLIVMFFWMAFHQNGTSLTYWANDNTDRVLSIGSWSMEIPPGVFQAF
                     NSAFIIILTPVIVRLMGFLRARNIEPSTPAKIGIGMVLTAASYGIMVLASWAGGDHGK
                     VHMLWLIACYFVITIAELFLSPMGLSMVTKLAPRRMTAMLMGVWFIATAIGNKLSGYL
                     PARFWDRWSHAKFFTLLVITSLVAAGLLLSQFRRLKAAMPPEGPPDEDKKQADPEPAV
                     AAVADAGY"
     gene            6852..8189
                     /locus_tag="PKL17_09870"
     CDS             6852..8189
                     /locus_tag="PKL17_09870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45080.1"
                     /translation="MSSVEIVAQAALPGSPLLAPAEQYVIGPFLNQGGMASVFLGRHI
                     GRTGIETPVVLKRLRSELTHVPELRALLFAEAELQSRLQHPGIVRTTDLVVLDGEYYL
                     VMEYVRGGDLQLLLRRARRRGQRFSAAAALFIVRELLQALSYAHELRDPTGTPLHLIH
                     RDISPANVLISGAGEVKLSDFGIAHTDATVGSLRLRGKVGYMSPEQARQEELDPRSDL
                     FSLATVLYELLTAKRLFVGQTGESASRVYAAPILPPSSLCTGLPRQADEVVLRALSLD
                     KNERPQSAQKWYGQLLELSRRHGLLIDRSELASHLQELLGDDPDTWRLLEERSGTAQI
                     PSLLDEDELWDERGSDGTMGISVVRTVEFVTLKPSDVLIQSDVTAPVPTQSADSAVPL
                     ALPVAQSSSPATRIISATAPQRLLDTLRAPLIRLLVFSLLSFVAAALVWLLLR"
     gene            8272..8892
                     /locus_tag="PKL17_09875"
     CDS             8272..8892
                     /locus_tag="PKL17_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45081.1"
                     /translation="MATPLTASQRQRFPSLAHYLDRVPQGLSSYPDCQAKVSLVRSML
                     EEHSLGKFAPELPPPLAELILQPPPLSAWMPEVHLRAILRIIHDRVLTDRRAFVKWTY
                     DAQKRLLSGPMYRLLFSVMSMDRLGDSAPSRWTRFHRGSTMQVEIGEKRSKVSFAFPS
                     HLFDDLDLEASLAGLQVAIEMMVGKSAEFSDMQVSPTSSCAVVFWR"
     gene            complement(8929..9002)
                     /locus_tag="PKL17_09880"
     tRNA            complement(8929..9002)
                     /locus_tag="PKL17_09880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8966..8968),aa:Arg,seq:cct)
     gene            complement(9084..9563)
                     /locus_tag="PKL17_09885"
     CDS             complement(9084..9563)
                     /locus_tag="PKL17_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45082.1"
                     /translation="MMRPRVRFRQLAVSLGSLLFFVSAAPVDAKNEPKPTRPICPLKC
                     PDGFHAALYTWELHPERTVYDETGKENVPDDGIWKVSCAVRCELKEGEKVTVKEEKKD
                     LCDSGGEPAPYVGPWRITGKWVRECAALFPDACGMSCYPKKLPPPPKKKPGAKRKKK"
     gene            9699..10205
                     /locus_tag="PKL17_09890"
     CDS             9699..10205
                     /locus_tag="PKL17_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45083.1"
                     /translation="MTDTAKPAPQPLTVALTRLLASFPRRKMPDKYLGMYFYMNASLQ
                     IHDLLDTWTRHEVKQLKIGPFTMDDAAIQKSFRLPVWVKEERGPHADCSIDETQVRQD
                     VILLGTYADTDAVVNLGLMRGPDEVLDKVIGISGPRNYHYGTLESFLATCQKRAAAQG
                     LPSALILS"
     gene            complement(10185..11705)
                     /locus_tag="PKL17_09895"
     CDS             complement(10185..11705)
                     /locus_tag="PKL17_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK45084.1"
                     /translation="MEIFERMGWYGFYAVSTLYLTSPVADGGLGLSSKDRGVIQGLST
                     FFLYFFPVLFGTLADRYGYRLMFALSAAVMVPGYLLLHLPHTFYGFFLVYILVACGHG
                     MFKPVVLSTIVKTTTEKTGTLGFGIFYMMVNIGGLIGPLVAGIVRGWSWDYVFYASAF
                     WIALMGVLSLVLYREPPHERQKESLSEVLRKMVAVVGNVRFFSLLMGMLLLLVLGNKW
                     WTIGRASAVAVGWLLFHLLWDVGIRLYLSQSRTHDVPWYRQTLRIGEGKYLLFLLLMS
                     GFWTCENQLAMTLPEYIRDYVDSRDLVASFQPLATLFCGLLHTVGVDTSSWSRAVLDH
                     GSVKPEHLINLNSLGIIFLQVVISYAARKLPPLVTIMSGVLLTAASFLLFVPFGKQGT
                     GWLVVIGVLSFSVGEMLASPKSKEYAGRIAPPGKVGLYMGYFYLCVALGNLFGGLLSG
                     AAYQHFGKQGSDRPDLLFALIAGLAVGSALLLWLYDRYLRKQQNAGDRILLGQDEG"
     gene            11821..12906
                     /locus_tag="PKL17_09900"
     CDS             11821..12906
                     /locus_tag="PKL17_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNK45085.1"
                     /translation="MSLLFERFPQLAARLPFRSLGNFPTALERVDGLVSSRIDLWIKR
                     EDQSGTLYGGNKVRKLELLLGAVSDDVLHGDPEKKPRLLTLGAWGSNHAIAVALYGQK
                     LGLAVDTVLFPQPLTVETAPYLQKQLLSQLAAEATLWPARTFVSVPATFLRAYLSAAG
                     PLVHIPAGGSSAAGVLGWVSGGMEIAAQLAAQGERPFDVVYVTIGTGGTAAGLWLGLG
                     DAARALRGVRVVPWPIASESGVRLLTRNAQRLFHKLTGQTLTPQPGFSVDGRFVGPGI
                     GYGAVTAASRDAVGRARAMGLALETTYTGKTLAALLADADSGLLDGKRVLFVNTASSV
                     DLSVLRQRADLHKLPPWLFRKLQSAKM"
     gene            12909..14141
                     /locus_tag="PKL17_09905"
     CDS             12909..14141
                     /locus_tag="PKL17_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNK45086.1"
                     /translation="MSAPLFAPYDEGGGFCCMPFGGQIQPPIRVLVIDDEAGVRRIFC
                     RLLTRAEMEVTEASDGFEALAILEKQRFDVILSDITMPGLSGVALLEAIRKLDLTTQV
                     IYATGNPSLETAVRAVELGAMRYLTKPIDHRQLVETVQEAARLHRLAELKQQAMSLLG
                     SGHPLASDRMGLQTAFLRVLEHLTLHYQPVVSVRSGRIWGYEALLRSADPALPSPHAV
                     LEAAEQLQKLPDLGRRVRGLAAQAAARLPDGVRLLVNLHPSDLEDPDILAPNAPLTAY
                     AARVILEVTERADISGKRELENHAAALRSLGYGVAIDDLGAGYAGLLSFLQLRPQVAK
                     LDMGLIRNIHKDPRRQAVTQSMIQLCRELNVEVIVEGIETVEERDVLRDLGCDLMQGF
                     LFARPAPDFVTLSLEPRP"
     gene            14138..16294
                     /locus_tag="PKL17_09910"
     CDS             14138..16294
                     /locus_tag="PKL17_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNK45087.1"
                     /translation="MSASLPPDGPPAGTTSSTKTERHISLDGLPIASLTVDRMFRDSL
                     IALFLLGVCALPMVWFVDFERASRAIFVCQVLSLLIGSAFGLALQMSSARVASNIASA
                     CVLASVCLGLQGGPLSAAWPLLALVPMLLLGLQYGRRGVVFSTFALITAGCFWTLTQG
                     RIADASSSSSLPILLAHVVGRSLVIGLSGFVIEHLLAIVRERETFLINVFRDIDIGVS
                     VFDVDDSGELRVAALNPRIYQLLGLPQKRLLGKKHHELLQDGQLQATEPMLRRAVSTG
                     KPQHNEDRLRSVDKHQRLLFTAVPQRDQEQQAVTRLVVSGTDITVLKAAERKLQMQAE
                     VLANVADAVIAVDGAFRVVYVNRAAERIFSVQSKTCLGQPLEEITGLEFLQQLKQHAG
                     QTKSSQSGTFVDPNGRELALDFSVSTQPDEDGDGPQYIAVIRDVRDRRSLEMQLLRAQ
                     KTDALGRMAGGIAHDFNNMLVVISGNAELLAESLPPDHDGQADLREIKEAAHRAAALV
                     RQLLAFSKRQTGLPRSTNPNQVLRDIAQILRRMLRDEIELELKLADGLWSVIIDVAQL
                     EQVMVQLLVNARDAIPKTGKVTVTTRNVELGPEAEQHELTPGRYVELTVKDTGIGMSA
                     ETMLHLFEPFFSTKASSRAVGLGLSVCYGIIRQHRGVITAQSKPGEGTTFVIWLPEES
                     TRPKDDRAIPQGSVSDEFRRLRPSTLDAGANKNHVK"
     gene            complement(16355..16486)
                     /locus_tag="PKL17_09915"
     CDS             complement(16355..16486)
                     /locus_tag="PKL17_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45088.1"
                     /translation="MRKTPNKLWSRKVVLSLLAVILLSFDSGCIVRPWGHRHHHHRW"
     gene            16670..17248
                     /locus_tag="PKL17_09920"
     CDS             16670..17248
                     /locus_tag="PKL17_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45089.1"
                     /translation="MNRLVALLGLWVCAAPLPAHSQTTPEHQEKTVVHSAAVDYGSFV
                     YLSPGLPIQAGGAALQYRARFSVGVAVSAGVRFLHVDRLPDHFGYEGFVGVQLAPIFG
                     SWRPLAGLEVGATSLQSAALSPSPSYSPEEYTSKQSPLGPVYAGFVIAPLRFGFKRFS
                     FLALGIQLATHLPKWGSATRLSFLVGQFEVSF"
     gene            17248..18252
                     /locus_tag="PKL17_09925"
     CDS             17248..18252
                     /locus_tag="PKL17_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNK45090.1"
                     /translation="MKTSYPKQAWATGVLAASLSFSSCQPDGFSASVTALSKQAASEV
                     ESAQLSLHVSALVNSRQMETAISSPLWGGMPLRRKNAASYMDSYFRGIGLAVQHEREV
                     QNEIETENLYVDLPGHDRKEELVLVTGHYDNWHLGADDNASAVAVLLETARILRSRNL
                     SRTVRIIAFDREEEGLIGSDRYARAHVGEKIVGILNMDCVGFASRALYSQTAPPGLAL
                     RSVGDFLAIIASDASADMLSKVTKLSTELPLPNPVLGIVAPGDGHNAAGSAFLRSDHA
                     PFWQKGIPALFLTDTANYRNPNYHRPGDLPDTLDYEFLSRNAALVVASAVALAEEGSR
                     "
     gene            18249..18851
                     /locus_tag="PKL17_09930"
     CDS             18249..18851
                     /locus_tag="PKL17_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45091.1"
                     /translation="MTRRLRTSSLCWALGIAIPKLLYAQVLPSSAEAAPGLAADTAGS
                     RPVSEPKKEVYVPAGLALFSGDAHLFAGVGGGIGFRYDLGPIWTLYTEAKANYYAGAS
                     GTLAFGISGRFVYRSYRPELGIGALLFVGEDVRILDSADASLVPPLAFAVTTRIAPLR
                     FVRGRYAASALTVDVGCGMDTRSRCALALSVNLLEAGLRF"
     gene            complement(18891..20285)
                     /locus_tag="PKL17_09935"
     CDS             complement(18891..20285)
                     /locus_tag="PKL17_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45092.1"
                     /translation="MRHRASLLSKVLGLCTTLGMTSCGEHVVVVELSNLATNTTTVSA
                     YYRIESDAYHASSLTSSQFPVGNPAFGIRPPEGRSGTMTVQVFAYKNNAPCELSKGST
                     TVDVSGSGKLSATAALDAGAAAPGCSANDAPVKYPSDAKVWAAAANNVWIVGKDAAVL
                     HWNGSLFKEISVPSSVLNGKTPTWNAVHGDSQGNVWLASDADSVVRIDQSGTYSGFTL
                     NFGSSTKNGIVWTGVYASGGQAILSATTTADGNGYIGTVGVGQNVISVTQTTQAPISL
                     PAVASYGLHSIGCSSLDDCWFGGNQSVIVHYTKAAGYKTTLLASGSSCTSQNSSVRVS
                     AIYANAAVAKVKMLGHTSDGSDLGSRAGVFLTYAPAAAPNPDCFWSSDQDSSSMYVPG
                     PLLGISGTSPDDLVVVGTDAMYRWKTSTPVPIPGIDKTSWQSVSAVPGGFFAVSQRSM
                     TDPMGRVYYADLGP"
     gene            complement(20316..20858)
                     /locus_tag="PKL17_09940"
     CDS             complement(20316..20858)
                     /locus_tag="PKL17_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45093.1"
                     /translation="MLCGSVCHAEDFDALFRRGMTLYQKKDFEAAIDAFQAAYKIRQF
                     PRVLLNIAQVYRKMGDAQLALDYYQQFLKAEPNPPAKIKLDVEQYITQTKAMLAATSL
                     KDEEERRREPAPTGYDKKTGQIMPWYVEMKDTEKQRKKKLLIGIICGSVAAAAAIGVG
                     VGVGLYYRNKLPDGITIFEY"
     gene            complement(20952..>21675)
                     /locus_tag="PKL17_09945"
     CDS             complement(20952..>21675)
                     /locus_tag="PKL17_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45094.1"
                     /translation="WEMLTGRVPFDGGHPTATMLKHLTDRPKPPSEIAQNQRIPPELD
                     QLVLRTMAKEPKERFASMAELEEALATQFSQLKQRSPSTAGLQLVRLAPPPTSLGRLT
                     AVLSLGAVVLFGGGLSLRWLIQRRAATMPPKLESRQPTHVHWRVETQPSGAEVWNVTD
                     GHKIGVTPWQQDEPLGEGALVIELRKSGFVAKQLTLSKKSDEDRTEPLAPRKEKDRGS
                     GTKKSGKKRKTRDNSQVELIPD"
BASE COUNT         4097 a         6204 c         6647 g         4727 t
ORIGIN      
        1 ttgccgcagt gctgacagta cataaaacct gccggcgtcg gatgtccgca gtgactgcat
       61 gcgatcttgg tctggggccg ttgcttcagg ttcaggtttt gcccacagct gcgacagaac
      121 gacgccgaga tcgggtttgt cgtgccgcag ctttcgcacg gcgagtccgc cgaagccgaa
      181 gtgccaccga gcccagcttc tttggtcggg ctgcgctcgc tgccgggcgc gggactttcg
      241 ggcgtgctga ccacctgtgc ggcggcgctg cgtcctattg gaagcggctc gtcgtcatgg
      301 gcgatgggga tcgatgccga tgctgccgta gcggcaaccg cggcaacgcc accccctggg
      361 ccataagccg agccgtcgat tgatctgcca cagtcttggc agaacagcgc gccgtctcga
      421 ttggcttttc cgcagttctt acaccgtgcc atcgcgtggt aaccgggatc ttgctctccc
      481 tatattaact agacagagaa ggcggcagcc aagcgcaaaa tccgtgtttg ctgctttggg
      541 gtcgcaaaat attcccgttt ggccgaccgc acttcggctg gcacaagggc ccaggcggtg
      601 gatttgccga ccgctgcgga cagcctaggc ggcggctgcc tcgaatgttt gcagggcgat
      661 ggtaggtgcg ctaaaacctc agtccctaca tgagtcgaag cacgcccgcg ccaaggtcca
      721 ttccgtctgc tgagaatcgg acgatcgcaa aatcccgtcg atcgctttcc catgttgcga
      781 acttggttgt gcgacggtgg cttgtgtttg gacttgggct ttgggcgatg ctttttgggc
      841 ttgccggctg catgggtccg gtggccaaag cgtcgtttgc caagcgactt ggatcggggg
      901 aagccggcaa cttgctcggg ccgtttgatg gactggtcat cgatcagagc acgtccaatc
      961 cgctcgccag cgcgctcgtc gtcgggactt gggcatttca agaaaccggc gggctggcgg
     1021 ttccggaatc atcgtatacc gtcaccgccg tcacgggcag cgacggttcg tattcgctac
     1081 cgagcctgcc ggcagggcga cagtttgccg ggcttttgcg gcgcttcacg ctcgtcgtct
     1141 acaaagcggg cttcgtgggc tatcgctccg atctgcgctt tgaggatcgc tcaccgcgac
     1201 acaacttcgt tcagcgtggc aatgtcgtgc ggctcgatcg ctttccagcg aatgaatcgc
     1261 atgtgcgcca cctggtgttt ctcggcagtg gtgcggcgct gcgtggcgcg gcccaggctg
     1321 aaatcatcgc ggcgtcgctg gaactgcaag atgcggcacc caagctggcc gccgaagaaa
     1381 gcgcgccaag tgacaccacc gcaccaaaac cggcgacggg ggattccgca gtaaaggaag
     1441 gaagctgaga tgcgacggca gcaagtccga cgctttttgg gcgctttcgc attggcgctt
     1501 ttgtcgcaat cgcccggccc aagcttggcg tatgaaggtg cgacgaccct cgctgggctg
     1561 accgaacaag cggcgctcaa gtcgcggctg caccggcgga tgctggaacg gtttgcgctg
     1621 tcgttggggc tttttgaacc gctgcggctc gatccggcgc tgatcggcga agacaaagcg
     1681 cgcagcctgc tgattcgact cttggcgctc gacgcgggac agggttacgc gccggagccg
     1741 ctgccgcgca aggccggtca gagctttgct ccgctgcgac tgcatgtgct tggctggctg
     1801 gctgccggcg cggtattgga gcagtcaccg gcgcagcgcg ttcggcacca cttcgctgac
     1861 tgcaagaccg gcggcgggct ttcgcgtcca gcgggaatga cggcgggcgc ggcgcggacc
     1921 gaagcggttt cgcaggggct gtcatcggta cgtcagctcc tcgcgggcgc ggcgatggat
     1981 ggcacggggc agtcggcgat ggactggatc gagtcacccg aaaacgagct ggggctggcc
     2041 acgttccttg atgcgtatga gcgttcggtg gtggcggaaa cggcggaagc gcgggaaagc
     2101 tcgctcgtcg aagcgctgct ggcagcgggc gcgatgctgt cggtgatcga gcaagctggc
     2161 gatccggcct atgtccatag cgatctgtcg gcggtgcttg cgggtgacta ttcgcattac
     2221 gtcgccgagc agtttggtcg ggctgggatt ccagaacccg atgccaagct cgatgtgccg
     2281 tctccgcagc ggctgcgcga tctgattttc gatggcaaag gcaacgggct ggcggaacga
     2341 accgggcggt ttttgtcgtc gggaatgatc tcctcgtcag tgcttccagc ggggaccaag
     2401 ctggttgggc caggtggcta tctttcgacg ccggcagcga agcactggct tttgtggggt
     2461 cagccgccac gcctttcgga cggtgaatgg ccggcgccgc ggtttgtgct cgatgagcgc
     2521 tgcttcgccg attacgcgac ggcacttttg cccgaagtgg gccgttatgc gcaggctggg
     2581 ctcgactttt tgctgcgtgg cgatctgcgg atcgcgatgc agggcgaaac gcagcttcag
     2641 atcaaactgc tcgatgaacc gctcggcagt ggcgtcgtca ccctgatcgg tgaaaagtcg
     2701 tcgggagagc gacaggtgtt aaagacgctg tcgacgctgc cgtcgcgacc tggggtgctc
     2761 ggactggtgc cgcttggcga tgtatcgatc aaagactacg cgcggcttgt ggtgctgtgg
     2821 aaaggacgcg atcggcaagg ccagagcttg gtaagctcgg cccaggttgc gctgcacaag
     2881 acggacgctg tggctcccgc cgcggcgacg acgacggaag cggccagcga gtaggtttac
     2941 ttttccaggt cggctgcaaa ctcggggcga tggttgctgg cggcgcccca gcgggtcttt
     3001 ttccacttgt cctggtacgc catccacaga cccatcgcat cggtgacaaa gcgcagcgcc
     3061 agtggctgtt cttggtttag ctctttttgt tcgcccggat cgtcttgtcc gtcgtagagc
     3121 ttgacctgtc ccgaaccgcc cacccacagc ttgtagcgtc cgagccgcat gacatgcgca
     3181 agctcatact ggctggcgat cgctggacgc ggatagccgc cgctgacccc ttgcgcgatg
     3241 ggaatcagcg attcgccctg gacatctttg ggaatcggca ttccgaacgc atcggcgagc
     3301 gtcgggagaa gatcgaccag ttccgcacct tcttcgacga cttttccgcc agggaacagc
     3361 ggtggatagt aaatccacag cgggatgtga atcagctctt ggcgcagcga ctggccgtgg
     3421 ccgattcgtc cgtggtccca aaactcctcg ccgtgatccg aggtgtagat gatcatcgtg
     3481 tcgttttcgt gcccggtctt tttgagcagc tcgagcagct tgccgaagtg atgatcgtta
     3541 taggagatgt cgcagtcgta cagcgcgata acccgctggc ggtcacgcgc cgtcaccgtc
     3601 agttgcccgg cgacgatctt gtcgagctgc gggtccatgc agcctttcac aaacggaccg
     3661 gagtacggat tcggatcgta tttcgagatc cacggctcat aggcacgcca cgagacgtgg
     3721 gcgtcgatgg ttcctagata caggaaaaac ggcttggtct tgccggggcc tttttcgatg
     3781 aagtttgccg cgtcgccgac aagctctgcg ctcttgagtc cgccgccttc gtggatgtgg
     3841 tttttcagca gatcccagcc atcgccaaaa ccccagcgct tggtgatgta gccgttggcg
     3901 atgtgcgcga tggtgtaaag cccggcttgc ttcatcacct cggggatgat gacgaagctg
     3961 tcgggcagga ccgctttgtc ggaaatgaac ttgtgctgcg acggatagag tccggtaaac
     4021 agcgaagcgt ggctgacccg cgattcgttg ccttgcgaat aggtgacggc aaaacgggtg
     4081 gcgcgcttgg aaaactcatc cagcaccggc gtttcgacgc gcgagctctt gttccacagc
     4141 ttgaacttgt cggcgcgggt gtcatcggac agccaaaaca ccacgttttt cggcttttcc
     4201 gttttgcgca gctgcggggc agaaccagcg gccagaagct gcgctttgtc gagcgcaatt
     4261 ttgtcgcacg aaccttcggc gcgcagaccc aagcgacgga tttcgccgcc atgactgccg
     4321 agcgccacag gcgaaccatc cagactgact gcttgcggcg caccgcgttt ttccggctgc
     4381 ggaccttcca ccgtgagctt gagcgcgcac gcccccgcat ccccttttag cgccagcgcg
     4441 ccgcctttgg gaacaaacgc atagtaatga agcgcaccac cgcccggaat cagcagtcga
     4501 ttgccatcgc tcagcttggg cattgtgacc gcagaagccg gcggagtgcc gcccacctga
     4561 atccattcca ctgccgcggc tgctttgtga cttgcgccgc cgccgatcgc aaaggatccg
     4621 ctctgcgcaa acgtcaggct cagcgtgttt tcgcccgcgt gcagcgaatc gggcggtagc
     4681 gacaccgtga caagctgcca gccttcggcg agtggaaccg cggtgctgct tttgcttccc
     4741 actgcgattt tgagcgacga cttgatcggg ctcttgagct gcaaatacac cacttggttt
     4801 gtggcttggc cgctgtgcag cggaacctga agcttggccg ctgtcgttgg cagcgcgact
     4861 tttttgccat cgacttcggc acgcagcttc caagttggcg tgggccgtcc aaagtgtgca
     4921 tagcgagcaa agcccgcgct gccagccgga atgacaagac cgccgcgctg ttcgatgtgc
     4981 gcgagcgttc gattggcgag cagatcaaac accggatagg acgccgccgg atggggcgct
     5041 tccgccgggg cttgcgcatg ggcgcttgcg gtaagcagca actggcagag tccgaaaaaa
     5101 agtcccgcga gcgttccccg cccgcagcgc agccgctttt gcgttggcgt tccgatcaac
     5161 ttctctgtcc tcccaaaaat atttcgcacg gcgggggaac atacacgcac ggtgggccga
     5221 aaaccaaggg tagggccagc attttgcagc gctggcgggc tgaagaagcg gagcggcacg
     5281 atgaactgag cccggcaaga aacgaccggc ggggagcggg ggttagcatg agcaaccgct
     5341 atcgggacca aagtgcaaac ttgtgggatt ttagggcttt acaagttccc cgtggctcat
     5401 gccaaatgag aggtacttaa atggcgagca agcacccgcg cggcctttac gtacttttct
     5461 tcacggagat gtgggagcgg ttcggcttct atttgatgct ggcgctcttt acgctgtacc
     5521 tgaccgaaca ccttcggatg acgaaggaag aagcgtctga tctgtacggc tcgtacatgt
     5581 tcttcgtgta cttgaccccg tttttcgggg gagtcctggc ggaccgcttg cttgggtaca
     5641 cccgtgccat cttgatgggc gcggcgctgc tcggggcggg ctacctggtc ttttcgatcc
     5701 agaaccagac tgcgttttac gcggcgctgt ttctgatggt gattggcaac ggtctgttta
     5761 agcccaacat ctccaccctg gtcggaaatc tgtatccgac gggcgatcct cgccgagact
     5821 ccgcgttttc gatcttttac atgggcataa acctcggggc gctgttcgcc ggcccggtgg
     5881 gtgagttcat gcgcaccaag ttcggttggc cgctggcgtt tgcgaccgcg ggcatcggca
     5941 tggtgttttc ctttttgatc tttgcggcgc tgcgcaactg ggtcacgctc ggcgatactc
     6001 ggtcgtcggt ggctgcggtt ctggatgtgc cgctgccggc ggaatacgaa gaccgtcccg
     6061 atccgcccga agtcgagcgg cagcgcatca tcgcgctgac catcatgtgt ctcatcgtga
     6121 tgttcttctg gatggcgttc caccagaacg gcacttcgct gacttactgg gcgaacgaca
     6181 acaccgaccg cgttctgtcg attggttcgt ggtcgatgga gattccgccc ggcgtgttcc
     6241 aagccttcaa ctccgcattc atcatcatcc tgaccccggt catcgttcgg ctcatgggct
     6301 ttttgcgcgc gcgcaacatc gaaccgagca cgcctgccaa gattggcatc ggcatggtgc
     6361 tcaccgcggc gtcatacgga atcatggtgc tggcgtcgtg ggccggtggc gaccatggca
     6421 aagtccacat gctgtggctt atcgcctgtt atttcgtcat cacgattgcc gagctcttcc
     6481 tgtcaccgat gggtctttcc atggtgacca agctggcgcc gcgtcgcatg accgccatgc
     6541 tgatgggcgt ttggtttatt gccaccgcga ttggcaacaa actgtcgggc tatctgccgg
     6601 cgcgcttttg ggacaggtgg tcgcacgcca agtttttcac cttgctggtg attacctcgc
     6661 tggtggcggc gggtctgctt ctgtcgcagt tccgtcggct caaagcggcg atgccgcctg
     6721 aaggtccgcc cgacgaagac aaaaagcagg ctgatccgga acctgcggtc gctgccgtcg
     6781 ctgacgccgg ctactaattt ccgtcccacg ttctgagcgg agaacctgga cccctaggta
     6841 ggtgtgtcgc tgtgtcttcc gttgaaatcg tagcgcaggc cgcgctgccc ggctcgccgc
     6901 tgttggcacc ggcagagcag tacgtgattg ggccgtttct caaccaaggg ggaatggcct
     6961 cggtgttttt gggcaggcac attggccgca ccggaatcga aacgccggtt gtgctcaagc
     7021 ggctgcgttc ggaactgacc cacgttcccg agctgcgcgc gctgctgttt gccgaagccg
     7081 agctccagtc gcgacttcag catccgggca ttgtgcgaac cacggatctg gtggtgctgg
     7141 atggcgagta ctacctggtg atggagtacg tccgcggcgg tgacttgcag ctgcttttgc
     7201 gacgggcgcg tcggcgaggc cagcgttttt ctgcggcggc ggcgctgttc attgtgcgcg
     7261 agcttctgca agcgctgtcg tatgcacacg agctgcgcga tccgaccggc acaccgctgc
     7321 atttgatcca tcgcgatatt tcgcctgcga atgtcctgat ttccggtgct ggtgaggtaa
     7381 agctttcgga ctttggcatt gcccataccg atgcgacggt gggaagcctg cggctgcgcg
     7441 gcaaggtcgg ctatatgtcg cccgaacagg cccggcagga agagctggac ccgcgttccg
     7501 atttgttttc gctggcgacg gtgctgtacg aacttttgac cgcgaagcga ctgtttgtcg
     7561 gccagaccgg agagagcgcc agccgggtct atgccgcacc gattctgcca ccgtcgagtc
     7621 tgtgtacggg cctgccgcgc caagccgatg aagtggtcct gcgcgcgctg tcgctcgaca
     7681 aaaatgaacg tccgcagtcg gcgcagaagt ggtacgggca gcttcttgag ctgtcgcgcc
     7741 ggcatggtct tttgattgat cgatccgagc tggcaagtca tcttcaggaa ctgttggggg
     7801 acgatccgga cacctggcgg ctgctcgaag aacggtcggg gacggcgcaa atcccgtcgc
     7861 ttttggatga agacgagctg tgggatgagc gtggcagtga cggaacgatg ggaatctcgg
     7921 tggtgcggac ggtcgagttt gtgacgctca agccgagcga tgtactgatc cagtccgacg
     7981 tgacggcgcc ggttccgacc caatcggcgg attcggcggt gccgcttgcg ctgccggtgg
     8041 cgcagagctc ttcacccgcg acgcggatca tttcggccac tgcaccccag cgactgctcg
     8101 ataccctgcg cgcgccgctt atccgactgc ttgtcttttc gcttctgtcc ttcgttgctg
     8161 cggcgctggt gtggctgctt ctgcgctagc ttgccttgtg cggcttgggg atgacacggg
     8221 ccctttctgc gtgcgaacat ccttttcgcg gcactaccga ggcctggccc catggctacc
     8281 ccacttactg cgtcacagcg acagcgattt ccatcgctgg cccattatct cgacagggtg
     8341 ccgcaggggc tttcgtccta tcccgactgc caggccaaag tgagccttgt gcgctcgatg
     8401 ctcgaggagc attcacttgg caagtttgcg ccggagctgc cgccgccgct cgccgagctg
     8461 atattgcaac cgccaccgct cagcgcctgg atgcccgaag ttcacctgcg ggcgattttg
     8521 cggatcatcc acgatcgcgt gctcaccgac cgacgcgcgt ttgtgaaatg gacgtatgac
     8581 gcgcagaagc ggctgctttc gggaccgatg tatcgcctgc tgttttcggt gatgtcgatg
     8641 gatcggctgg gcgatagtgc accgagtcgc tggacccgct tccatcgtgg ctcgaccatg
     8701 caggtcgaaa ttggcgaaaa gcggagcaaa gtgagcttcg cgtttccttc gcatctgttc
     8761 gatgacttgg atctggaagc gtcgctcgcg gggctccaag tcgccatcga gatgatggtc
     8821 ggcaagtccg ccgaattttc ggacatgcaa gtcagtccga ccagctcctg cgccgtcgtg
     8881 ttttggcgct aggagccgga ctgcgcaaac ccccaagttc cggctgtgtg accgcggcgc
     8941 ggttcgaacg cgcgacctac tgtttaggaa acagttgctc tatccagctg agctacgcag
     9001 tcgatgaagc cagcagccta tagcacgacg atccggtcag tggctacttc cttgacgagt
     9061 gcttttgccg cggcgtctct cggctacttt tttttgcgtt ttgcgccggg cttctttttc
     9121 ggcggtggtg gcagtttttt cgggtaacag ctcatgccgc aggcgtctgg gaacagcgcc
     9181 gcgcactcac ggacccactt gccagtgatt cgccacggtc cgacgtaggg cgctggctcg
     9241 ccgccgctgt cacagaggtc ttttttttcc tctttgacgg tgactttttc gccttctttg
     9301 agctcgcagc ggacggcgca gcttactttc cagatgccgt cgtcggggac gttttctttg
     9361 ccggtttcgt cgtagacggt gcgctcggga tgcagctccc aggtgtagag cgcggcgtgg
     9421 aagccgtcag gacacttgag cggacagatg ggccgggttg gtttgggttc atttttcgca
     9481 tccaccggcg cggcgctaac aaaaaacagc agtgacccaa gcgacactgc aagctggcga
     9541 aatcgaacac gggggcgcat cattgaccta tcaccatcgt cgcacagcgc gccatttccg
     9601 gcaatcgcgg cgatcttggg caatggctcg cgcaaccgca actgggtgtg acaaagtgct
     9661 cggcgctggc tggcgagcga tctagtatga agtgacacat gaccgacaca gcgaaaccgg
     9721 ctccgcagcc gcttacggtt gcgctcaccc gacttttggc gagctttccg cggcggaaga
     9781 tgcccgacaa gtacctcgga atgtactttt acatgaatgc ctcgcttcag attcatgacc
     9841 tgctcgatac ctggacgcgg catgaagtaa agcagctcaa gatcggaccg tttacgatgg
     9901 acgatgcggc gattcagaag tcgtttcgtc tgccggtctg ggtcaaagag gaacgcggcc
     9961 cacatgccga ctgctcgatc gacgaaacgc aggtgcggca agacgtgatt ctgcttggga
    10021 cctacgctga caccgacgcg gtggtaaacc ttggtcttat gcgtggtccc gatgaagtgc
    10081 tggacaaagt gatcggaatc agcggaccgc gcaactatca ctacggaacg ctcgagagct
    10141 ttttggctac ctgtcagaaa cgcgccgctg cccagggact tccgtcagcc ctcatcttgt
    10201 cctaaaagaa tcctatcgcc cgcattctgc tgcttgcgca gatagcgatc atacagccac
    10261 aaaagcagcg cgcttccgac ggcaagtccg gcgatcaggg caaacagcag atccggtcga
    10321 tcgctgccct gtttgccaaa gtgctgatac gccgcaccgg acagcagtcc gccaaacaga
    10381 tttcccagcg ccacgcacag ataaaagtag cccatgtaaa gaccgacttt tccgggtggc
    10441 gcaatccgtc ctgcgtattc ctttgatttc ggactcgcga gcatttcacc gacggaaaag
    10501 gacaggactc cgatcaccac cagccagccg gttccttgtt tcccaaacgg cacaaacaga
    10561 aggaagcttg ccgcggtcag gagcactccg ctcatgatgg tgacaagcgg tggtagcttg
    10621 cgtgccgcat aggaaatcac cacctgcaaa aagatgattc ccagcgagtt taagttgatc
    10681 aaatgctcgg gttttacact cccgtgatcg agcactgcac gcgaccatga agaggtatcc
    10741 acaccgacgg tgtgcagaag tccgcagaag agtgtcgcca gcggctgaaa gcttgcgacc
    10801 agatcacgcg aatcaacgta gtcccggatg tattccggaa gcgtcatcgc gagctgattt
    10861 tcacaagtcc aaaaaccact catcagcaag agaaacagca gatattttcc ttcgccgatg
    10921 cgcagcgtct gccgatacca cggaacgtcg tgagtccgac tttgggacaa ataaagacgg
    10981 attcccacat cccacagaag gtggaacaga agccagccca cagcgacggc ggaagcccga
    11041 ccaatggtcc accatttgtt gcccaagacc agcaaaagca gcatgcccat cagaagcgaa
    11101 aagaagcgca cgttgccgac cactgcgacc attttgcgca gcacttccga aagcgattcc
    11161 ttttgccttt cgtggggtgg ttcgcggtac agcacaagcg acagcacgcc catgagcgca
    11221 atccaaaatg cggacgcata aaagacgtaa tcccagctcc agccgcgcac gatgccagcg
    11281 acgagcggac cgatgagtcc gccgatgttg accatcatgt aaaagatgcc aaagccgagc
    11341 gttccggtct tttcggtcgt ggtcttcaca atcgtactga gcaccacggg cttgaacatg
    11401 ccgtgaccgc aggcgaccaa aatgtagacc aggaaaaatc cgtaaaacgt gtgcggcaag
    11461 tgcaagagca gatagcccgg aaccatcact gccgccgaca gcgcgaacat cagccgatag
    11521 ccgtaacgat ccgcaagcgt tccgaacagc accggaaaaa aatacagaaa gaacgtcgac
    11581 agaccctgga tgacaccgcg gtctttggat gaaagaccca gaccgccatc ggcaacgggc
    11641 gacgtcaggt acagcgtcga cacggcataa aaaccgtacc agcccatgcg ctcgaaaatt
    11701 tccatcgcgt tggcgaccca gaacgagcgt gggaagtcgg gaagctttcg atcagaaatt
    11761 gccgagggag gctgcatgta agtggctgat ctgccagcgt ctaacacata ggatggcggc
    11821 atgtcacttt tgtttgagcg ttttccgcag cttgccgccc ggctgccatt tcgctcgctt
    11881 gggaactttc cgacggcact agagcgcgtc gacggacttg tgtcgtcccg gatcgatctg
    11941 tggataaagc gcgaggatca atcgggcacg ctgtacggtg gcaacaaagt ccgcaagcta
    12001 gagcttttgc ttggcgccgt gagcgacgac gtgctgcatg gtgatccgga aaaaaaacca
    12061 cgactcttga cgctcggggc gtggggatca aaccacgcga ttgcggtggc gctgtacgga
    12121 cagaagctgg ggctggcggt cgatacggtg ttgtttccgc agccgctcac cgtcgaaacg
    12181 gcgccgtatt tgcaaaaaca gctgttaagc cagcttgcgg cagaagcaac gctgtggcca
    12241 gccaggacat ttgtatcggt gccggcgacg tttttgcgcg cgtatctgtc ggcagcgggt
    12301 ccgcttgtgc acattccagc gggtggatcg tcggcagccg gtgtgctcgg ttgggtgtcc
    12361 ggagggatgg aaattgccgc gcagctcgcc gcgcaagggg aacgtccgtt tgatgtggtc
    12421 tatgtcacaa tcggaacggg cggaaccgcg gcgggtttgt ggctgggact tggcgatgcg
    12481 gcgcgggcgc tgcgcggagt gcgggtggtg ccgtggccga ttgccagcga aagcggagtc
    12541 cgtctgctca cgcggaatgc acagcggctg tttcacaagc tgaccggaca gacgctaacc
    12601 ccacaaccgg ggttttcggt cgacggacgt ttcgtcggac cgggaattgg ctatggcgcg
    12661 gtgacggctg cgtcacgcga tgcagtcggg cgcgcacggg cgatgggtct tgcgctggaa
    12721 accacgtata ccggcaagac gttggcggcg cttttggccg atgcagacag cggactactc
    12781 gacggaaaac gggttctgtt tgtgaacacc gcatcgagcg tggacctttc ggtactcagg
    12841 cagcgtgcgg atttgcacaa gttgccgccg tggctgtttc gtaagctgca atctgccaaa
    12901 atgtgaactt gtcagcaccc ctttttgccc cctacgatga aggtgggggt ttttgctgca
    12961 tgccgtttgg cgggcaaatc cagccgccca tccgcgtgct ggtgattgat gatgaagcgg
    13021 gggtccgccg cattttttgt cggctgctca cgcgcgccga aatggaagtg acggaagcga
    13081 gcgacggctt tgaagcgctg gcgatactgg aaaaacagcg cttcgatgtg atcttgagcg
    13141 acattacgat gccgggtctt tcgggggtgg cgctgctgga agcgattcgc aagctggacc
    13201 ttaccaccca ggtgatctat gcgacgggga atccgtcgct ggaaaccgcg gtgcgcgcgg
    13261 tcgagctggg cgcgatgcgc tacctgacaa agccgatcga tcaccggcag cttgtcgaaa
    13321 cggtgcagga agcggcgcgg ctgcaccgac tggcagagct gaaacagcag gcaatgtcgc
    13381 tgctcggatc gggacatccg ctggcgtccg atcggatggg tttacagacg gcatttttgc
    13441 gggtcttgga gcacttgacg ctgcattatc agccggtggt gtcggtgcgc agcggacgga
    13501 tttggggtta tgaagcgctg ctgcgatcgg ctgacccggc gcttccgtcg ccgcatgccg
    13561 tgctcgaagc ggcagagcag ctgcaaaaac tgcctgatct ggggcgccgt gtgcgcggtc
    13621 tggcggcgca agcagcggca cgacttcccg atggtgttcg tctgcttgtg aacttgcatc
    13681 catccgatct cgaagatccg gacattctgg ctccgaatgc acctttgact gcgtatgcag
    13741 cgcgggtgat cttggaagtc accgagcgcg cggacatctc gggcaagcgc gagctggaaa
    13801 accatgcggc agcgctgcgc agcttggggt atggcgtcgc gattgatgac ctgggcgcag
    13861 gatatgcggg gctgctcagc tttttgcagc ttcgacccca ggtggccaag ctcgacatgg
    13921 ggctgattcg caacatccac aaggacccgc gccgccaggc tgtcacccag tcgatgatcc
    13981 agctctgtcg cgagctaaac gtagaggtca ttgtcgaagg catcgaaacc gtcgaggagc
    14041 gcgatgtcct gcgcgatctg ggctgcgatc tgatgcaggg ctttctgttt gcccgtccgg
    14101 cgcctgattt tgtcacgctg tcgctggagc ccaggccgtg agcgcttctc tgcctcccga
    14161 tgggccacct gcaggaacca cgagcagcac aaaaaccgag cgacacatca gtctggacgg
    14221 acttccgatt gcgagcttga ccgtcgatcg gatgtttcgc gatagcttga ttgcgctttt
    14281 tttgctcgga gtctgtgcgc tgccgatggt ctggtttgtg gactttgagc gcgcctcgcg
    14341 agcgatcttt gtttgccagg tgctttcgct cttgatcggt tccgcttttg gacttgcact
    14401 gcaaatgtcc tcggcgcgag ttgccagcaa cattgcgtcc gcgtgcgtgc ttgcttcggt
    14461 ctgtttgggt ctgcaaggcg ggccgctgtc ggcagcgtgg ccgcttttgg cgctggtgcc
    14521 gatgcttctg ctcggtcttc agtacgggcg gcgtggtgtc gtgttttcga cgtttgcgct
    14581 gatcacagcg ggctgttttt ggacgcttac gcagggtcga atcgcggacg catcgtcgag
    14641 cagttcgctt ccgattctgc ttgcgcatgt ggtgggccgc agcctcgtga tagggctttc
    14701 cggttttgtg atcgaacacc tgctggcgat tgtgcgcgag cgcgagacgt tccttatcaa
    14761 cgtgttccgc gacatcgaca ttggcgtgtc ggtgttcgat gtcgacgaca gcggcgagct
    14821 gcgggtggcg gcgctcaatc cgcgcattta tcagctgctg ggactgccgc aaaagcgact
    14881 gcttggcaaa aaacatcacg agcttctgca agacggacaa ctgcaagcca ctgagccgat
    14941 gttacggcga gcggtgtcga cgggaaaacc gcaacacaac gaagatcgct tgcgttcagt
    15001 ggacaagcac cagcggctgc tcttcaccgc ggttccgcag cgcgatcagg aacagcaagc
    15061 ggtgacacgg ctcgtggttt cgggaacaga catcacggtg ctcaaagcgg cggaacgcaa
    15121 acttcagatg caagcggaag tgctcgccaa tgttgcagat gcggtcatcg ccgtcgatgg
    15181 agcgttccgg gtggtgtatg tgaatcgtgc agcagagcgg atcttttccg ttcaaagcaa
    15241 gacgtgtctg ggacagccgc tcgaagaaat caccgggctt gagtttttgc agcagctgaa
    15301 acagcacgcc ggacaaacca agagcagtca gtcaggaacg tttgtcgatc caaatggaag
    15361 agagctggcg ctcgattttt ctgtttccac ccagcctgat gaagatgggg acggtccaca
    15421 gtacatcgca gtgattcgcg atgtgcggga tcggcgatcg ctggaaatgc agcttctgcg
    15481 cgcgcaaaaa accgatgcac tgggtcggat ggcgggcggt attgcgcacg actttaacaa
    15541 catgctggtc gtgatttcgg gcaacgcgga gctgcttgcc gagtcgctgc cgcctgatca
    15601 tgatggccag gccgacctgc gtgagatcaa agaagcggcg catcgggcgg cggctctggt
    15661 ccggcagctg ctcgcttttt ccaagcggca gacagggctg ccccgatcaa caaatcccaa
    15721 ccaagtgctg cgcgacattg cgcagattct gcggcggatg ctgcgcgatg aaatcgagct
    15781 ggaactgaag ctggccgacg gactgtggtc tgtgatcatc gatgtggcgc agcttgaaca
    15841 ggtgatggtg cagcttttgg tgaacgcgcg cgatgcgatt cccaagaccg gaaaagtcac
    15901 ggtgaccacg cgcaacgtag agcttggccc cgaagcagag cagcacgagc tgacccccgg
    15961 tcgatacgtg gaacttacgg tgaaagacac cggaattggg atgtctgccg agaccatgct
    16021 gcatctgttc gagccgttct tttccaccaa agcatcgagc cgcgccgtcg gtctgggtct
    16081 gtctgtctgt tacggaatca tccgccaaca tcggggtgtc atcaccgcac agagcaagcc
    16141 gggcgaaggc actacgtttg tgatctggct tcctgaagaa agcacccggc ccaaagatga
    16201 tcgggcgatc ccgcaaggat cggtgagtga tgagtttcgg cggctgcgcc cctcgacgct
    16261 ggatgctggt gcaaataaga atcatgtgaa atgactctta ttttgcaggc atccaccgcg
    16321 ccatcataca caagcgccaa aacatgcgtg cggactacca gcgatgatga tggtggcgat
    16381 gtccccacgg acgaacaatg cagccactgt caaacgacag cagaatgact gcaagcagac
    16441 tcaagaccac tttgcgagac cacaacttgt tcggtgtttt gcgcatgaca gcctccgatg
    16501 gatcacttcg tttggatagg tgaagcagct tctgcttgct gtttgaacac cgcaacgtga
    16561 caaaactgtc accgcgatgt cccgtcgata atgccgcttt tttaggctgc atgggatcgc
    16621 tgccgtacaa aaactgcgac cttggatctg gccgtggtaa gagagccgga tgaaccggtt
    16681 ggttgctctg cttgggcttt gggtgtgcgc ggctcctttg cctgcacatt cacaaacaac
    16741 gcccgaacat caggaaaaaa cagtcgtcca ttcggctgct gtcgactacg gatcgtttgt
    16801 gtacctaagc cccggtttgc ccattcaagc gggaggcgca gcgctacagt atcgggcgcg
    16861 gttttccgtc ggggttgcgg tgtccgcagg ggttcgtttt ctgcatgtcg acagacttcc
    16921 cgatcacttt ggatacgaag gttttgtcgg cgttcagctt gcaccgatct ttggttcgtg
    16981 gcgtccgctg gccggtctgg aagtcggcgc gaccagcttg cagtctgcgg ctctgtctcc
    17041 gtccccgtcc tacagtccgg aagagtacac aagcaaacag agtccgctcg gcccagtcta
    17101 tgccggattt gtgattgcac cgctgcgttt tggcttcaag cgcttttcgt ttttggcgct
    17161 cggaatccag ctcgcaacgc atcttcccaa gtggggcagc gcgacccggc tttcgtttct
    17221 ggtcggtcag ttcgaggtgt cgttctgatg aaaacgtctt atccaaaaca agcgtgggcg
    17281 acgggagttc ttgcggcgtc gctatcattt tcaagctgcc agcccgatgg tttttccgcc
    17341 tctgttactg ctctgtcgaa gcaggccgca tcggaagtag aatccgccca gctgtctttg
    17401 catgtgtctg cacttgtgaa cagccggcaa atggaaactg cgatctcatc gccactttgg
    17461 ggaggcatgc cgctgcgacg gaaaaatgcc gcaagctaca tggattccta ttttcgggga
    17521 attggtcttg cggtgcagca cgaacgcgaa gtgcaaaatg agatcgagac agagaacctc
    17581 tatgtggatc ttccaggtca tgatcgcaaa gaggagctgg tcttggtgac aggtcactat
    17641 gacaactggc acctgggcgc tgatgacaat gcatctgccg tcgctgtttt gctggaaacg
    17701 gcgcgaattt tgcgcagccg aaacctgtcg cgcacagtcc gcatcatcgc gttcgatcgc
    17761 gaagaggaag gactcatcgg cagcgatcgg tatgcacgcg cgcatgtcgg ggaaaaaatc
    17821 gtcggcatcc tcaacatgga ctgcgtgggt tttgcaagtc gcgcgctgta cagtcaaacg
    17881 gccccgccgg gtcttgccct gcgcagcgtg ggtgattttt tggcgatcat tgcaagtgat
    17941 gcctctgcgg acatgctgtc caaagtcacc aagctgtcga cggagcttcc gttgccaaac
    18001 ccagtgcttg gaattgttgc accgggggac ggacacaatg ctgcgggatc ggcgtttctg
    18061 cgttccgatc atgcgccgtt ttggcaaaaa ggaatcccgg cgctgttttt gaccgacacc
    18121 gcgaactatc gcaatcccaa ctatcaccgt ccaggcgatc ttccggacac gctcgattac
    18181 gagtttctgt cccgcaatgc cgcgctggtt gttgcgtcgg ctgtggcgct ggcagaggag
    18241 ggctcacgat gacgcgcagg ctgcggacaa gttcactttg ttgggcgctg ggaatcgcga
    18301 ttcccaagct gctttatgca caagtgctgc cgtcttccgc ggaggcagca ccgggtcttg
    18361 ccgcggacac cgcagggtct aggcccgttt cggaaccaaa aaaggaagtc tatgttcccg
    18421 cgggacttgc gctgttttct ggcgatgcgc acctgtttgc aggcgtcgga ggcggcatcg
    18481 gttttcgcta tgatctgggt ccgatctgga cgctctatac cgaagcaaaa gcaaactact
    18541 atgcaggcgc aagcggcacc cttgcgttcg gaatcagcgg acgctttgtc tatcgcagct
    18601 acagaccgga gctgggaatc ggggcgctgc tctttgtcgg cgaagacgtt cgcattctgg
    18661 attctgccga tgcatcgctt gtgcctccac tcgctttcgc tgtcaccacc cggattgcac
    18721 cgctgcgttt tgttcgcgga cgttatgccg cgtcggcgct gacggtggac gtgggctgtg
    18781 gaatggatac ccgcagtcgc tgtgcgctgg cgctgtctgt gaatctactg gaagccggtc
    18841 tgcgctttta ggaatcttcc gcacagatag gaaactgcaa tgggaaactg ctacggaccc
    18901 agatcggcgt agtacacgcg acccatgggg tcggtcatgc ttctttggga caccgcaaag
    18961 aatcccccag gaacagcaga caccgattgc cagcttgttt tgtcgatgcc cggaatcggg
    19021 actggcgtgg atgttttcca gcgatacatt gcgtcggttc caaccacaac gagatcatct
    19081 ggactcgtgc ccgatattcc aagtagcggt ccaggaacat acatgctgct actgtcttga
    19141 tcgctcgacc aaaaacaatc gggattcggc gcagctgccg gcgcgtatgt gaggaacaca
    19201 ccggctctgc ttcccaggtc tgagccatcg cttgtgtgac ccaacatctt gacctttgcc
    19261 actgccgcat ttgcatagat ggcgctcacc cgtactgaac tgttctgact tgtgcacgat
    19321 gagccgctgg caagaagggt cgtcttgtat cctgccgcct tggtgtagtg aacgatgacg
    19381 gactgattcc ctccgaacca gcagtcatcg agagaagaac aaccgattga atgcaggccg
    19441 tatgaagcga ctgctggcaa tgagatgggc gcctgtgtgg tttgagtgac agaaatgacg
    19501 ttctgtccga cgccaacagt cccgatgtag ccgttgccat cagcagttgt ggtggcggac
    19561 agaatggctt gaccgccgct ggcgtagaca cccgtccaga caattccgtt tttggtcgag
    19621 gagccgaaat tcaaagtaaa accagagtac gttccacttt gatcaatgcg gacgacagag
    19681 tcggcgtcac tggccagcca gacatttcct tggctgtctc cgtgaactgc attccacgtc
    19741 ggagttttgc cgtttagcac gctgctcggc acgctgattt ctttgaacag cgatccattc
    19801 cagtgcagca ccgcagcgtc ttttccgacg atccagacgt tatttgctgc ggctgcccag
    19861 actttggcat cgctgggata ctttaccgga gcatcgtttg cgctacagcc aggtgccgcc
    19921 gcccctgcat cgagtgcggc ggttgcagag agctttccgg aaccgctcac atcgacggtg
    19981 gtactgcctt tgctaagctc gcaaggtgca ttgtttttgt acgcaaagac ctgcaccgtc
    20041 atcgtgccac tgcgaccttc tgggggacgg attccgaagg ccggatttcc gactgggaac
    20101 tgactgctcg tcagtgaact ggcgtgatag gcgtccgatt caattcgata gtaggcagac
    20161 acggtcgtgg tgtttgtagc caagttgctc agttccacca ccaccacatg ttcaccgcac
    20221 gaggtcattc cgagcgtggt gcagagcccc agaacttttg aaagaagact cgctctgtgc
    20281 cgcatgttgt cttgccctct gcgcgatcga tgtccctagt attcaaaaat cgtgattccg
    20341 tcgggaagct tgtttcggta gtagagtccg acgccaaccc caaccccaat cgccgctgct
    20401 gcagcaaccg atccgcatat gatgccaatc aaaagcttct ttttgcgctg cttctcggtg
    20461 tctttcatct ccacatacca cggcatgatc tgaccggtct ttttgtcata gccggtcggg
    20521 gcaggctcgc gacgacgttc ctcttcgtct ttcagactcg tcgctgcaag catcgctttg
    20581 gtctgggtga tgtactgttc aacgtccagc ttgatcttgg ccggtggatt cggttcagcc
    20641 ttcagaaact gctgataata gtcgagcgcc agctgggcat cacccatctt gcgatagacc
    20701 tgggcgatgt tgagcagcac gcgcggaaac tgacggattt tgtatgccgc ttggaatgca
    20761 tcgatcgcgg cctcaaagtc ttttttttgg tacagcgtca tgccgcgccg aaacagtgcg
    20821 tcaaaatcct cggcgtgaca gacgctgcca cagagcatcg cgaacagaaa aatagaggaa
    20881 tataaaatgc ctcttatgtt tactcgccga tgtagagctg cggatcgcat gcgcatggca
    20941 gacactcttt tttagtccgg aatcagctcg acttggctgt tgtcgcgggt ctttctcttt
    21001 ttgccgctct ttttggtgcc agatcccctg tccttttctt tgcgcggtgc gagcggctct
    21061 gttcgatcct catcactttt tttgctgagc gtaagctgct tggcgacaaa accggacttg
    21121 cgcagctcga tcaccagcgc gccttcaccg agcggctcgt cttgctgcca cggtgtcact
    21181 ccgatcttgt gtccgtccgt gacattccac acctcggccc cgcttggctg cgtttcgacg
    21241 cgccagtgta cgtgcgtcgg ttgccggctt tcaagcttcg gcggcatcgt tgctgcccgt
    21301 cgctgaatca accaccgcaa gctgagcccg ccgccaaaca gcaccaccgc acccagcgaa
    21361 agcaccgccg tcagccgacc cagcgacgtc ggcggtggcg caagccgcac aagctgaaga
    21421 cccgctgtcg acggcgatcg ctgcttgagc tgcgaaaact gcgtcgccag cgcttcctct
    21481 agctccgcca tcgaggcgaa gcgctctttc ggctctttcg ccatggtcct tagcacgagc
    21541 tgatcgagct ctggcggaat gcgctggttt tgggcgatct cactcggggg cttgggccga
    21601 tcggtgaggt gtttcagcat cgtcgccgtc ggatgccccc catcaaacgg cacccgcccg
    21661 gtcaacattt cccac
//
LOCUS       DAORGN010000072        21410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_973_length_21410_cov_7.244533, whole genome shotgun sequence.
ACCESSION   DAORGN010000072 DAORGN010000000
VERSION     DAORGN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 21410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21410
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_21410_cov_7.244533"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1755)
                     /locus_tag="PKL17_09950"
     CDS             complement(211..1755)
                     /locus_tag="PKL17_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45095.1"
                     /translation="MRKLAFPLAVMSFSLGLGSCQKSPPSDDPPPAPPRLVWQKTQVS
                     TDSIDFPARRRGLRAVRGIIHLHSVYSHDACDGNPQPSGQPNAPCLRNLRKGLCDTRQ
                     DFAMLTDHATHMGEADWDKLFLTDSGLGDEKIIEAGEQTGGKMNCPSGDGSHQVIITV
                     GGENDLMPVGLHHHLGDTVSARKQAMSADTPLAVKAFHDAGGLTLQAHGESRDRTKMC
                     TLAAAGLDGMEVYNLHANLDPKIRETYLGLDGLAAFAGLAPWLEARPVADGGPEPDLA
                     LLGFLEDNSKERDHYNALLGAGYRPFPTFGSDIHENTFKGAMADGERGDSYRRLMRWF
                     ANYLLVPARSTLLPSDLSTAIQKGRGFSVFEAMGQPVGFDFFAEKTDGSRAELGDAVD
                     PGAKLTVRPPNPLPVGYATDEVVETLRVMFVPAGSSVAEALVDEKLTAAQLQQGQVRT
                     IDTAARGAGAYRVEVRLVPKHLLHLLGEEPSRYDKEYPYLFSGSIFVGTVPKHGGSCK
                     SVPLPS"
     gene            1816..2946
                     /gene="mnmA"
                     /locus_tag="PKL17_09955"
     CDS             1816..2946
                     /gene="mnmA"
                     /locus_tag="PKL17_09955"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNK45096.1"
                     /translation="MSKRERIVVALSGGVDSATCAGLLVEAGHEVVGISMRLYNAKGT
                     VSASGGRCCGPRDLEDARVVCAHLGIPFYVADYEEEFHRTVIDDFVATYQRGETPNPC
                     VRCNEQVKFLPLLYRARALGATALATGHYARLEGEKGARRLLRGVDGRKDQSYFLFGM
                     PRSALDFVEFPLGGMTKDEVRGHARRLGLPVSEKPESQEICFVPDGDYAGFVERQGGQ
                     SPPGGDVVDERGKVIGHHEGVHHFTIGQRRGLGFGVGSPQYVVGIDALSRRVKVGPKE
                     SLYAGRALVSQVRWLGEPPGDLLRCGVQIRYRRVPQSATVQLTGEQTAAVVFDSPELA
                     VTPGQAAVFYDGDRVLGGGFISESAPAAVAPRVSDAEAALSP"
     gene            complement(2909..4357)
                     /locus_tag="PKL17_09960"
     CDS             complement(2909..4357)
                     /locus_tag="PKL17_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45097.1"
                     /translation="MLVSGQVLSGTYLVERPLGSGGMADVYLVRHTRLSRHFALKVLK
                     LELSSRPGLIERFRREAEVLARLRSPHIVGVVDLNHTDEGWPYLVMEYLSGETLAARI
                     KRQGKLPIEAAVDLFGQLCDGVLAAHERDVIHRDLKPNNIFLSPGPRGDLVQILDFGI
                     AKLCEEGRTPLTRDSVVMGTPGYMAPEQALGHKDEIDFRVDQFALGAILYEMLAGKPA
                     FFVPGEPMMRTMQRLVYEDPKPLPESTLSPLLRRALNKRPADRYPSLRDLLADLRSLP
                     TPRWLSPSEISPPAYERQLHEPHRTKRVWPVAALVFAVGVSAVSALWLGHRAPASPTP
                     TPAVAPAPVAIVSPAPAATPPHDAPLVNLPKDETTRTPPPALTKPTIPAPISKPRTAQ
                     KLHRSFVISGATSAQEKLLRLCADKELRELPGLYSTTLRLERTGALQLTQAPEVVYRS
                     GFDACLRQAFAGTRTPLPELVTVKVQLQRQKP"
     gene            complement(4381..5676)
                     /locus_tag="PKL17_09965"
     CDS             complement(4381..5676)
                     /locus_tag="PKL17_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK45098.1"
                     /translation="MTTSKLALFGLTWALSSCAVNLCDKDPSDPRCEQNPILPDTLQV
                     MPTRIANTGTHITVRLGTGAGTVVLKQGGKQLSLGSLSDGFLEVHVTTAMLQQANISL
                     GAAQIVVSRPTKADSTQTISVFAEPQFDPAISYDTQPDGEIPEGVAIKPTGGIWGFTS
                     FPRGGGRAQHFVEYQISSGQLISRGPAFGNYNFAAWTDHPARTVFQGKRLVTFSRDLF
                     SGSDAIQLDLCGLNPDLCSPLSVTPDFTKVLGLTTDVKGTMLAVQSDQDVYVYQSSDS
                     SPIDRRQNIMGTSSRAGQKIALGDINGDGRIDLVTVSDQGGSVFLGQAGGGLSYDSTM
                     SGKLTGALAGSVPTALTAADMDADGSDDLIVASGTSLQILYPLPGGAFTVSSALPGLD
                     GADTISVGPVDGKSNSGPDIAISSEKAQRIAVIINQSSY"
     gene            complement(5673..6758)
                     /locus_tag="PKL17_09970"
     CDS             complement(5673..6758)
                     /locus_tag="PKL17_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45099.1"
                     /translation="MTIREKARKARCAEGLWLASLLGLGLFVLPLAAHAKEKVLLLSV
                     LKEGRPDPKLLRVAQDRLQKTGEQLVQSNDLSNTERKCMQQDCLLALAASKGADVVLA
                     GTVQKGKGGSQSLGVLLYDFGKMKASELSGDCDKCTPETTTQRLHELFVRALSEFRGA
                     APTSQAPLVAKNDKPQPGAEGQAQQAQANAQTDTGLKQPFPVGPAQAQAQVQPILPPQ
                     VQARPGEEPLAVVTGGPLPPKTQPVEPDQKSNPAIDIVQKQPQTVSHGLQLSPRRKIL
                     AGVFGGLALGSLVAAVALTATDGNTTSMDCSAAPGVPKVCVLDNKVPSSIGYVAAGAF
                     TVGTVLTLLWPESKPVAKESTSAEAAK"
     gene            complement(6921..8981)
                     /locus_tag="PKL17_09975"
     CDS             complement(6921..8981)
                     /locus_tag="PKL17_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNK45100.1"
                     /translation="MPRDLPIGNGHLLFNFDSEYRIRDVYYPFVGKENHAGGHVFRFG
                     VMCNQKFRWVGKKEGWDIQLGYESDTLLTDVKLHHPELGLSLHVSDCVDFHEALLVRR
                     ITVENHADSASEVRLFFHQDFRLSESEIGDTAAFDPRNNTVVHYKGERYFLVNLLCDG
                     EVGIQHYAIGQKGFPGREGTWRDAEDDGVLSGNPIAQGTVDSVVAAHTWVPAGKSKVI
                     YYWMIAAHAWAGRWDGLHEVNAKVKERGPQSFIERTRAYWRLWARKEPIDFAELAEPL
                     VDLYRRSLLILRTQIDNHGAIIAANDSDVTQFNRDTYSYLWPRDGALVAHALDLAGYP
                     EVSLRFFQFCADVLTQDGFLLHKYNPDKSLASSWHPWIIGKEPVIPIQEDETALVVWA
                     LWKHFQRYRDLESVKPLYGRLIRKAADFMVRYREPLTRLPAPSYDLWEERRGILSFTC
                     AAVFAGLRAAAEFARTFGEEDNAVRYSQAASEIREGMDAFLFRPELGRFARMINVTAS
                     RSIEVDGALDASLWGLFYFGAYPADDPRVAATQAQVVAGLSCKTEVGGIARYRGDYYH
                     QVSSDFDKVPGNPWFICTLWQADHLIAKAQNQAELESALPILTWVANHKLRSGVLAEQ
                     VHPHTGAPLSVSPLTWSHATYVSTVQAYLEKREQLGLRGVMPPFRKLKALDLGDFGHV
                     HEKS"
     gene            9167..9796
                     /locus_tag="PKL17_09980"
     CDS             9167..9796
                     /locus_tag="PKL17_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK45101.1"
                     /translation="MSLTLDARLERVLRFLALATTGKLSDRIPLEDDGEERLLELEYG
                     VNMLFDELERSRTESAAQQRELEQQSAALLEKEREMVRLLSTPVITVWPGVLALPILG
                     EVGRERAVTMTESVLTQVVQERATHVILDLTGMTVVGAETAMSLLRLAQSVRLLGGRC
                     LLTGVSGPLASVLVGLSFESSDVVALPTLADAIAKVLRERGEKLVTQSK"
     gene            9809..10162
                     /locus_tag="PKL17_09985"
     CDS             9809..10162
                     /locus_tag="PKL17_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNK45102.1"
                     /translation="MPISIEEKKTPKGQRLMLAKVSGHVSYADAESMGNQLKPGQPFH
                     QALVLCVVDKSTDYHPDARRHFNTFNGNYKKLATVVTSVLIRAAINFMMRVSGVGQDA
                     VLFNTEADALAWLDG"
     gene            complement(10198..11595)
                     /locus_tag="PKL17_09990"
     CDS             complement(10198..11595)
                     /locus_tag="PKL17_09990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK45103.1"
                     /translation="MQIHFLGAAEEVTGSMYLLELAQGTILIDCGFFQGRREESHRRN
                     ARLPKEATQADVAILTHAHIDHSGNLPTLVKSGFQGSIYATPATRDLCAYMLRDSARI
                     QEADAAYLNRKYKDDPDFSPIAPIYDEEDVIKALERFVGMPYHHTFYPLPGVRAEFYN
                     AGHILGSSEAVLTVEENGTRRTILFSGDLGRKGLPIIRDPEYPPPPIDYVVMESTYGN
                     RVHGSILQMEDDLERVIKETVRRGGKVIVPAFAVGRTQELLYALHSLSTQHRIPPIPV
                     FIDSPLAINVTEVFKLHPECFDQETRAFLSAHGEVFDYSQVRFVSSREESLRLNDLKG
                     SAIIISASGMAEAGRILHHLRNNVEDERNTILIVGFMAQHTLGRRLAEQRPRVKIWGV
                     ERDLHARVEVMNSFSAHADRRDLLEFARRCGTETRTFFLVHGEPEAQKPLKDEMTQKG
                     HRVRIPSRGEVISLE"
     gene            complement(11640..12677)
                     /locus_tag="PKL17_09995"
     CDS             complement(11640..12677)
                     /locus_tag="PKL17_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45104.1"
                     /translation="MDLGPEPSADLGPLPTALPVAGNPDGKCAVPTEAQAEVSTAPTT
                     VIGNGTAQSCTSDAVVAAVAKGGIITFNCGPDPITITMTQTAKIFNDRGPKIVIDGGG
                     KVTLSGGGKVRILYQNTCDAAQKYTTTHCQDQDHPQLTVQNLTFVDGNAKGLSPGGGG
                     AIFASGGRFKVVNSRFFRNVVDDTGPDVGGAAVRVLQQSMGLPAYVVSSTFGGEGGLG
                     NSGSNGGGLSSIGVSYTVLNSLFAQNAAIGIGANPPRSGTPGGGSGGAIYCDGNLFTL
                     TLCGVKMTGNTAKEGGGAIFFVSNNRTGSLIIKDSLLSGNPSQGFETAGYKGIFFLGS
                     GTPQVTNSTIQ"
     assembly_gap    12727..12826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12912..14867)
                     /locus_tag="PKL17_10000"
     CDS             complement(12912..14867)
                     /locus_tag="PKL17_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45105.1"
                     /translation="MNSDSEKAPTENRVHALWARLRRAASSIDGSVWAVWIIAILHYR
                     FTRGIFQGKGSGDGFFGFQYLPGLVFHHSFDLAQAAPQWTAIIGRETTGLVANPCPIG
                     PLLFWFPFYLLGLLLQKLALVPGLGILLSALVPTLKPAQVLSGQTEADLYMAGLGSLF
                     AGLWGIRILFDFLCRHFGQTAARFATCSAVLATPLFWYLTTQPLYQHATAFFAVTMLV
                     DLSDRYRHDLSVRRCLVLGFWSGIAALQRPQEALWMLPLALWMLGLVVSDLRMRNGKR
                     ALAIIGKGILIMSVAALLFVPQVLLWLHYYGVLRAPQKPGHFLWDTPAVVESLFSLRA
                     GIFPWVPLTYLSACGLLIALRRHRALTLPLFLTAILELYVNASAWDFHGSWSFGPRRY
                     TDAVVVFAVGLGALFVWLLDRIPKQKQPVSRLLWGLLVLFASFNLMMVELVRQGRNKS
                     SSAGAFSAATRVRWAKGPEFLARLLERTGYPFLQPVSAWYALAYQIPLVRVEDLLGSY
                     VNERDWRIREVVHTPSIVVAENPPQVVEGLSLSRIAGEGNVKSRIRVLIPLVVSEPLR
                     LTLEGSFPGSCSALSVRWNGRRLPIEKEPGPALPAGQESQRAIHIQIDQPAVFARPRF
                     NELIVEDIPIGSKLTRITTTSTTRWWK"
     gene            14966..16573
                     /gene="gltX"
                     /locus_tag="PKL17_10005"
     CDS             14966..16573
                     /gene="gltX"
                     /locus_tag="PKL17_10005"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNK45106.1"
                     /translation="MTAPASSSESTLGSNLFAARWPGRDRRPVRVRIAPSPTGDPHVG
                     TAYIALFNYVFAKKHGGQFILRIEDTDQTRSTLASEDAILRALRWIGLKWDEGPDCGG
                     PFGPYRQSERTDIYQQHAQMLVDKGAAYPCFCTSDRLEASRKARMAEGKPPGYDRLCR
                     YLSKEEVAARKAAGDKYVIRLAMPLEGETRFYDGLRGDIVIDNATIDDQVLLKSDGFP
                     TYHLANVVDDHLMQVSHVIRAEEWIISTPKHVQLYAAFGWEPPDFQHMPLLRNPDRSK
                     ISKRKNPVSLDYYRDAGFFPEALLNFLSLMGFAFGGDREKFTLAEMIEAFDWTKVSAG
                     EPVFDLQKLTWLNGLYLREMPPETLLERLRAWRLSDSYLLSLIPLLRERIERMDQFIP
                     SASFFLTGDLDYSAVLSQLIPKGRDQLQVSQILSELTDYLEEHVRTGWTQELLDKACR
                     DFCEKSGWKPKELFMPIRIAVTGRTASPGLFETMVAIGKDLTRRRLRLASQAVLTYSA
                     PAVASPAAQQKPSPQGKPAPAPTTKPA"
     gene            16590..17423
                     /locus_tag="PKL17_10010"
     CDS             16590..17423
                     /locus_tag="PKL17_10010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNK45107.1"
                     /translation="MLRRIPLLGFLLSAWDSFDQAALQHRSTLSAEQNAQEVKRIREL
                     LLVAGLLLTLGYCFGDRPFFEEVFGPFISRHPRLEHYHELLSFTYWSVAKLLGYGVFP
                     VLHIVLRRESPADFGLSLRGVSAVEEKIRLPWARMYLVLLALILPAVFAVSFTASFQQ
                     NYPFYGKASRSLFDFVVWETEYLSTFVAVEFFFRGYLLFGLRRYLGSLALFVSMVPYC
                     LIHVLKPAPEAIGSIFAGLLLGTLALCTGTLWSGVLLHVSVALSMDVLSSLQSGRLPK
                     F"
     gene            complement(17427..19085)
                     /locus_tag="PKL17_10015"
     CDS             complement(17427..19085)
                     /locus_tag="PKL17_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45108.1"
                     /translation="MSAAGDKQPVMVISWTGEADEKGYWKSLVKPQKSIKGSFVADKK
                     KKTYDLTADDSSKAVLAEYMKDPMFSVSLWNLIGEDLNALTVNGKALMQRSLGDQVWE
                     IAQWDVARSLFLNNTLFDKAPLKGEQDDDSKEWVLANGLTLPEKLMVSVNIAGKMVAK
                     AKDNEHTAAFLRDQVAEVFKPLVQALANDLKTIDAELPNDKDPKKAKSVKKANDRLDK
                     FRSDAETAIVKSVTDQWLRQLEANVDYRDFKVDTAITIGKGVISLTLGITTAAVGGIT
                     GVGAVLGLVGTIKGGIETAVAAYQAFRNVEKMGKDLTKVLAVAIESQSATGWKNFGKE
                     VLGKVPGGPALKEVLALGGVSVKSASDIEKELKTYNGKVSGLLVNAKDLGKKVADVVS
                     KSDATLKALDSKEVKEAEASNPALKKSNEKKRKAIAEATTKFLDEIGTLTQRFETGKK
                     SVDECGQLLDILKGKVKEDKVAGVLTKYFVPLLELPWGVDPTNVRASIQSAGSIGMDL
                     ASTALTDLASMGDVGEQAKDWMKFANDSINAIKGLQEIVADKKK"
     gene            complement(19629..19973)
                     /locus_tag="PKL17_10020"
     CDS             complement(19629..19973)
                     /locus_tag="PKL17_10020"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK45109.1"
                     /translation="MTSEKRHNPRRPIDILLNKYIDGQPHVCRGVNLSRGGMLVHKLF
                     EPDVPHLSVTIELELPGNPEVLCIEGVVLTAGRSARSMAIRFLRMPPRVARLIEQFVH
                     GQELREVAQASV"
     gene            complement(20070..20282)
                     /locus_tag="PKL17_10025"
     CDS             complement(20070..20282)
                     /locus_tag="PKL17_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45110.1"
                     /translation="MRQIAAPSVSLSDEPRTTATTDGPAVHGPDPDRLQIESHRFCPG
                     CSSELFDRGCKLRCPRCGFFLDCSDG"
     gene            20468..>21410
                     /locus_tag="PKL17_10030"
     CDS             20468..>21410
                     /locus_tag="PKL17_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45111.1"
                     /translation="MILSESARSLLALSVDLRDAVVLLVDDSPRDAEELLVALTEVGL
                     KVLVAESGPAAKQKLMREAVDLVIVDLKRADMDSFELTRQLKVPPQTRLLPVLFVSAK
                     KDIDSSIRAFRVGASDYIDTAIDFSELLVRIEFHIRHGRKLRELEREKAELARELREL
                     KKETAKQIAQIPVYTNLAELPSGFVIDGKYRLESLIGTGGFGVVYRATHTQLLRQVAV
                     KVFRPLTNLSNEDALRRFRHEGASASRLQHPNAVTILDSGVAPGGIPYLAMELLVGRT
                     LADELKEKRVLSLARTVQIILPVCDVLIEAHAATLVHR"
BASE COUNT         4211 a         6189 c         6386 g         4524 t
ORIGIN      
        1 ggccaaaggc ttccttggtt tcggtggtcg ccgcggcagt cgatccggct ggagtcgcag
       61 cagcagccgt cggagccgtg gtcttttcgg tggtgccagt gccgcttttt tgacagccgg
      121 cgagcgcgat caggctgacc agtccgacga ggggaagaca ctttcgggta cacatcgttg
      181 atgactccgg caatccttgc ggcgatcggc ttacgacggc aggggcacgc tcttgcagct
      241 gccaccgtgt tttggcaccg ttccgacgaa aatcgagcct gaaaacaggt acggatactc
      301 tttatcgtaa cggctcggtt cctcgccgag aaggtgcaaa agatgcttgg ggacaagccg
      361 cacttcgacg cgataggccc cggccccacg cgcggcggta tcgatggtgc ggacttggcc
      421 ttgctgaagc tgtgccgcgg tcagtttttc atcgacaagc gcttctgcca cgctgctgcc
      481 cgccggtaca aacatcacac gcagcgtttc gacgacttca tctgtcgcat atccgacggg
      541 aagcggattc ggcggacgca cggtgagctt ggcccccgga tcgacggcat cgcccagttc
      601 ggcgcggctt ccgtcggttt tttcggcgaa aaaatcgaaa ccgacgggct ggcccattgc
      661 ttcaaacacg gaaaagccgc ggcctttttg aatcgccgtc gacagatcgc tcggcagcag
      721 cgttgaccgc gcaggcacaa gcagatagtt ggcaaaccag cgcatcaggc gacgatagga
      781 atcgccccgc tcgccgtcgg ccatggctcc cttgaaggtg ttttcgtgga tgtccgatcc
      841 gaacgtcgga aacggtcggt agccagcgcc gagcagcgca ttatagtggt cgcgctcttt
      901 gctgttgtct tcgagaaagc cgagcagcgc gaggtccggt tcgggtccac cgtctgccac
      961 cgggcgcgct tcgagccacg gcgcaagccc tgcaaatgcc gccagtccgt cgagcccgag
     1021 gtacgtttcg cgaatcttgg gatcgaggtt ggcgtgcagg ttgtaaactt ccatcccgtc
     1081 gagacctgcc gctgcgagcg tacacatttt ggtccgatcg cgcgattcac cgtgggcttg
     1141 gagcgtcagt ccgccggcgt cgtgaaatgc tttcaccgcc aagggggtat cggcgctcat
     1201 cgcttgtttg cgcgcggaga cggtatcgcc caagtggtgg tgcagtccga ccggcatcag
     1261 atcgttttct ccaccgacgg tgatgatgac ttgatgactg ccgtcgccac tggggcagtt
     1321 catcttgccg ccggtctgtt cgccggcttc gatgattttt tcgtcgccca ggccggagtc
     1381 ggtcagaaac agcttgtccc agtcggcttc gcccatgtgg gtggcgtgat cggtcagcat
     1441 cgcaaagtcc tgccgtgtgt cacacagccc tttgcgcagg ttgcgcagac acggcgcgtt
     1501 tggctgaccg gacggctgcg ggtttccatc gcaggcatcg tgcgaataga ccgagtgcag
     1561 gtgaatgatg ccgcgcaccg cacgcagacc gcgccttcgc gctggaaagt cgatgctgtc
     1621 cgtcgagacc tgcgtttttt gccagaccag ccgcggcggc gctggcggtg gatcatcgct
     1681 gggcgggctt ttttgacagc tacctagccc gagagaaaac gacatcacag cgagaggaaa
     1741 cgcaagcttt cgcatgatgg cgcggactat acgccgactt gcacacggac aagaaagaaa
     1801 cctatgctgc gcgcgatgag caagcgggag cggatcgtcg tcgcgctatc gggcggtgtg
     1861 gacagcgcaa cgtgtgcagg acttctcgtc gaagccgggc atgaagtggt cgggatttcg
     1921 atgcgccttt acaacgccaa agggacggtc agtgcgtcgg gcgggcgctg ttgtggaccg
     1981 cgtgatctcg aagacgcccg cgtggtctgt gcgcacctgg ggattccgtt ttacgtggcc
     2041 gactacgaag aggagtttca tcgcacggtc atcgacgatt ttgtcgcgac gtaccagcgc
     2101 ggcgaaacac cgaatccgtg tgtgcgctgc aacgagcagg taaagttttt gccgcttctg
     2161 tatcgggcac gggcgcttgg ggcgacggca ctggcgacgg ggcactacgc gcgactcgaa
     2221 ggcgaaaaag gcgcgcggcg gcttctgcgt ggcgtcgatg ggcgcaaaga tcagtcgtac
     2281 tttttgtttg ggatgccgcg gtcggcgctc gattttgtcg aatttcctct cggtggcatg
     2341 accaaggacg aagtgcgtgg acacgcgcgc aggttgggtc tgccggtgtc ggaaaagccc
     2401 gaatcacaag aaatctgctt tgttcccgac ggggattacg cgggttttgt cgaacggcaa
     2461 ggcggtcagt cgccaccagg cggagacgtc gtcgatgagc gcggcaaagt gatcggacat
     2521 cacgaaggcg tacatcactt cacaatcggt cagcgtcgcg ggctcggttt tggtgtggga
     2581 tcgccgcagt atgtcgtcgg cattgatgcg ctttcaaggc gagtgaaagt cggacccaaa
     2641 gagtcgcttt atgccgggcg ggcgctggtt tcgcaggtcc gctggctggg tgaaccaccg
     2701 ggggacttgc tgcgctgcgg ggtgcagata cggtatcgac gggtgccgca gtccgcgacg
     2761 gtgcagctta ccggtgaaca gacggcagcg gtggtctttg attcgcccga gctggcggtc
     2821 acaccgggtc aagcggcggt gttttacgat ggcgatcgcg tgctcggagg tggttttatc
     2881 agcgaaagtg ccccggcagc tgtcgcgcct agggtttctg acgctgaagc tgcactttca
     2941 ccgtgaccag ctcgggcagc ggggttcgtg tgcctgcaaa cgcttgccgc agacaggcat
     3001 cgaagcccga tcgatagacc acctcgggcg cttgcgtaag ctggagcgcg ccggtccgtt
     3061 cgagccgaag tgtcgtcgag tacagtcctg gcagctcgcg cagttctttg tcggcacata
     3121 gccgcagcag tttttcctgt gccgacgtgg cacctgaaat cacaaacgag cggtgcagtt
     3181 tttgtgcggt gcgcggcttg gagatgggcg ctggaatcgt cggtttggta agcgcgggcg
     3241 gcggagtccg cgtggtttca tcctttggca gattcaccaa aggtgcgtcg tgaggcggtg
     3301 tcgcagcggg ggccggactg acgattgcaa ctggggctgg ggctacagcc ggggtcggcg
     3361 tgggcgaagc cggtgcgcga tgcccaagcc acagcgccga cacggcagac acgcccacgg
     3421 caaaaaccaa agcggcaacc ggccagacgc gctttgttcg gtgtggttcg tggagttgtc
     3481 gctcgtaagc gggtggtgag atttccgacg gagacagcca acgcggcgtc ggcagactgc
     3541 gcaaatcggc caaaagatcg cgcaaagacg gataacgatc cgccggacgc ttgttgagcg
     3601 cccgtcgcaa aagcggtgac agcgtggact cgggcagcgg cttgggatct tcgtacacca
     3661 ggcgctgcat cgttcgcatc atcggttcac cgggtacaaa gaacgccggc ttcccggcca
     3721 gcatttcgta caaaatcgct ccgagcgcaa actggtcgac gcgaaagtcg atttcgtctt
     3781 tgtgccccag cgcctgttcc ggcgccatat aacccggtgt gcccatcacc accgaatccc
     3841 gcgtcagcgg cgtccgccct tcctcgcaca gcttggcgat accaaagtcg aggatctgca
     3901 ccaagtcacc gcgcggcccc ggcgacaaga agatgttgtt tggtttcaga tcgcggtgaa
     3961 tcacgtcgcg ctcgtgtgcc gcgagcacgc catcgcagag ctggccaaac agatcgaccg
     4021 ccgcttcgat cgggagcttg ccctgtcgct tgatccgcgc cgccagggtt tcgcccgaaa
     4081 ggtattccat caccaaatac ggccagccct cgtcggtgtg gtttaggtca acgacgccga
     4141 caatgtgcgg actgcgcagc cgagccaaga cttcggcctc gcggcgaaat cgttcgataa
     4201 gccccggtcg cgaagacagc tcaagcttga gaactttgag cgcgaagtgt cgagacagcc
     4261 gggtgtgacg gaccaaatac acatccgcca ttccacccga gccgagcgga cgttccacga
     4321 gatacgtgcc agagagaacc tggccggata cgagcatgca gcgccttgtt tttcagggac
     4381 ttagtacgag gactggttga tgattacggc gatacgctgc gctttttccg aggaaatggc
     4441 aatgtctggg cccgagttcg atttgccgtc gacgggtccg acagagatgg tatcggcacc
     4501 gtcgagtcca ggcagggcgg aagacacggt gaacgcgcca cctggcagcg ggtaaagaat
     4561 ctgcaagctg gtgccgctgg cgacgatcaa gtcatcgctg ccgtcagcat ccatgtccgc
     4621 cgcggtcagt gctgttggca cgctgccggc gagcgctccg gtcagcttgc cgctcatcgt
     4681 gctgtcgtag cttaggccgc cgcccgcttg gcccaaaaac acgctgccgc cttgatccga
     4741 taccgtcaca agatcgatgc gtccgtcacc gtttatgtcg ccaagcgcga tcttttgacc
     4801 ggcgcgagag ctagtgccca tgatgttctg acggcgatcg atcggcgacg agtcgctcga
     4861 ctgatagacg tagacgtctt ggtctgactg gaccgccagc atcgtgcctt tgacgtcggt
     4921 ggtaagccct agcaccttgg tgaaatccgg cgtcaccgac agcggcgagc aaaggtcggg
     4981 attgagcccg cacagatcga gctgaatcgc gtcgctgccc gaaaacaagt cacggctaaa
     5041 cgtcaccagc cgcttgcctt ggaacaccgt gcgcgctggg tgatcggtcc acgcagcgaa
     5101 gttgtagttg ccaaacgccg gaccgcgcga aatcagctgt cccgacgaga tttggtattc
     5161 aacgaagtgc tgggcgcgac cgccgccgcg tgggaacgac gtaaaacccc agatgccgcc
     5221 ggttggcttt atcgcgacgc cttcggggat ttctccgtcg ggctgcgtgt cataggagat
     5281 cgccggatcg aactgcggct cggcgaagac cgaaatcgtt tgggtggaat cggctttggt
     5341 gggccggctg acgacgatct gtgccgcgcc aagcgaaatg ttggcctgct ggagcatcgc
     5401 cgtggtgacg tgaacttcga ggaatccgtc gctgagcgag ccaagcgaga gctgttttcc
     5461 accctgcttg agcaccacgg ttcccgcgcc tgtcccgagc cgcaccgtga tgtgcgttcc
     5521 ggtgttggcg atgcgggtcg gcatcacctg gagcgtgtcg ggcagaatcg ggttctgttc
     5581 gcagcgtggg tccgagggat ctttgtcgca caggttcact gcacacgacg acagcgccca
     5641 ggtgagacca aatagtgcaa gcttcgaagt ggtcattttg ctgcctccgc cgacgtgctt
     5701 tccttcgcca ccggcttcga ttccggccac agcagcgtca gcaccgtacc gaccgtgaac
     5761 gcaccggcag ccacgtaacc tatcgacgac ggaactttgt tgtcgagaac gcagactttc
     5821 ggaacccccg gcgcagcgct acagtccatg gatgtcgtgt tgccgtcggt cgccgtcagc
     5881 gccaccgccg cgacgagcga accgagcgcg agaccgccga acacaccggc caggatcttg
     5941 cgacgcgggc tcagctgcag accatgcgac acggtctgtg gttgcttctg cacgatgtcg
     6001 atcgcgggat ttgacttctg atcgggctcg accggctgcg tctttggcgg cagcggtccg
     6061 ccggtcacga cggccagcgg ttcttcaccc ggccttgcct gcacttgcgg gggcaggatc
     6121 ggctgcactt gcgcctgcgc ttgggctggg ccgaccggga atggctgctt gagccccgta
     6181 tcggtctgcg catttgcctg cgcttgctgc gcctgtccct cggcacccgg ctgtggcttg
     6241 tcgtttttcg cgaccagcgg cgcttggctc gtcggggcgg caccgcggaa ctccgacagg
     6301 gcacgcacaa acagctcgtg cagacgctgt gtggtggtct ccggcgtgca cttgtcacag
     6361 tctcccgaca gctccgacgc cttcattttg ccaaagtcgt agagcagcac accgagcgac
     6421 tgactgccgc ctttgccctt ttgcaccgtc cccgcgagca ccacgtcggc gcctttgctt
     6481 gccgccagtg ccaacaagca atcctgctgc atgcactttc gctcggtgtt cgacaggtcg
     6541 ttgctctgga caagctgctc gccagttttc tgcaagcggt cttgggcaac gcgcaaaagc
     6601 ttcgggtccg gcctgccttc cttgagcacc gacaggagca gcactttttc cttggcgtgt
     6661 gcggcgagcg gcaggacaaa aaggccaagc ccaagtagcg aggccagcca aagcccttca
     6721 gcgcaccttg ccttgcgtgc tttttcacgg atggtcacaa gattcctcca agcgtgatca
     6781 tggcagcttg ggtggggtct aagtcaagat cagttctcaa tttttccgcg atttcacata
     6841 cgaaacgagg ggatggtcgg cgtccggttt cactcggtct gcgaccctgc tcgcgtgcgt
     6901 tagatcacgc gggggatgtt tcacgacttt tcgtgcacat gtccgaagtc acccagatcg
     6961 agcgctttga gcttgcggaa cggcggcatc accccgcgca gcccgagctg ttcgcgcttt
     7021 tccagatagg cctgcaccgt cgagacatag gtggcgtgcg accaagtcag tggtgacacc
     7081 gaaagtggcg ctccggtatg cggatggact tgttcggcca ggacgcccga gcgcagcttg
     7141 tgattggcca cccaagtgag gattggcagc gccgactcca gctccgcttg gttctgtgct
     7201 ttcgcaatca ggtgatcggc ctgccacagc gtgcagatga accacggatt ccccggcact
     7261 ttgtcaaagt ccgacgaaac ctggtgatag taatcgccac gatagcgtgc gatgcctccc
     7321 acttcggttt tgcacgacag acccgcgacc acttgtgctt gggttgcagc gacgcgcgga
     7381 tcatcggctg gataggcgcc aaaataaaac agaccccaca gcgatgcatc cagcgctccg
     7441 tccacttcga tgctgcgcga tgcggtgacg tttatcatgc gggcaaagcg acccagctcg
     7501 ggacggaaca ggaatgcatc catgccttcg cgaatttcgc tcgcggcttg gctgtagcgc
     7561 accgcgttgt cttcttcccc aaacgtgcgc gcaaactctg ccgctgcgcg cagaccggca
     7621 aacacagccg cacaggtgaa cgacagaatg ccgcgccgct cctcccataa atcataggaa
     7681 ggcgccggca gcctcgtgag cggctcgcga tagcgcacca tgaagtcggc tgctttgcga
     7741 atcagccgcc cgtacagagg cttgaccgat tccagatcgc ggtagcgctg aaagtgtttc
     7801 cacagagccc agaccaccag tgcggtttcg tcttcctgaa tcggaatcac cggctctttg
     7861 ccgatgatcc acggatgcca cgacgacgcg agcgacttgt ccggattgta tttgtgcagc
     7921 agaaagccat cttgggtcag cacatcggcg cagaactgaa aaaagcgcag ggacacttcc
     7981 ggatatcccg ccagatcgag cgcgtgtgcc accagcgcgc catcgcgtgg ccacagatag
     8041 ctgtaggtat cgcgattgaa ctgcgtcacg tcgctgtcgt tcgccgcgat gatcgctccg
     8101 tggttgtcga tctgggtgcg caggatcaac agcgaccgcc gatacagatc gacaagcggc
     8161 tccgccagct cggcaaaatc gatcggttct ttgcgcgccc acagccgcca gtacgcgcgc
     8221 gttcgctcga taaacgactg gggaccgcgc tcctttactt tcgcatttac ttcgtgaaga
     8281 ccgtcccagc gaccggccca ggcatgcgcc gcgatcatcc agtagtagat gactttgctt
     8341 tttcctgccg gcacccaagt gtgggcagcg acgacagaat cgacggttcc ttgcgcaatg
     8401 ggattgccgg aaaggactcc gtcgtcctct gcatcgcgcc aggttccttc gcgtccgggg
     8461 aatccttttt gtccaatcgc atagtgctgg atgccgactt ccccatcgca cagcagattg
     8521 accaggaaat agcgttcccc tttgtagtga acgacggtgt tattgcgcgg atcgaacgcc
     8581 gcggtgtcac caatttccga ttcgctcagc cggaagtcct gatggaaaaa cagccgcact
     8641 tccgatgccg aatccgcatg gttttcgacg gtgattcggc gaaccagcag cgcttcgtga
     8701 aagtctacac agtcgcttac gtgaagcgac agcccaagct ctggatgatg cagcttcaca
     8761 tcggtcagca gggtgtcgct ttcatatcca agctggatgt cccagccttc cttcttcccg
     8821 acccagcgaa acttctggtt gcacatcact ccgaaacgga agacatgccc gccagcgtgg
     8881 ttttccttgc cgacaaacgg atagtagaca tcgcgaatcc ggtattcgga atcgaagttg
     8941 aacagcagat gtccgttacc aatcggaaga tcgcgcggca tgttccccct ttgccttgtg
     9001 gctgtgcgtg cggacagccg atccaatgta ctttgccgac gaaaaccaag tcaatgatgc
     9061 cgcggcccgc tgatttgttg gggggcgtgg ttggtgttgg cagatggggt ctcttgttgg
     9121 gtttgcactt tcttcctggt cgcgatcgga gatatgatcg attgcggtgt ctcttacact
     9181 tgacgcacgg ctagagcgtg tcctgcgctt tttggcgctg gccacaacag gaaaactgtc
     9241 tgaccgcatc ccgcttgaag acgacggtga agagcggctg ctcgaactag agtacggcgt
     9301 caacatgctg ttcgatgagc tggaacggag tcgaacagag agcgcggcac agcaaaggga
     9361 actggagcag cagagtgcag cgctgctcga aaaagaacgc gaaatggtgc ggcttctgtc
     9421 gacgccagtc atcacggtat ggccgggcgt gctggcactc cctatcttgg gggaagtcgg
     9481 tcgcgagcgt gccgtgacga tgacagaatc ggtgctgacc caagtggtgc aagagcgcgc
     9541 aacccacgtc attttggacc tgacgggaat gacggtggtg ggtgcagaga ccgcgatgtc
     9601 gctgttgcgg ctggcccagt cggtgcggct gcttgggggt cggtgtttgc tgactggtgt
     9661 gagcggtccg ctggccagcg tgcttgtcgg tctcagcttc gagtcaagtg atgtggtggc
     9721 gcttcccacc ttggccgatg cgattgcaaa agtgctgcga gagcgcggcg aaaagcttgt
     9781 gacgcagagc aaataagagg aatagaaaat gcctatatcc atcgaggaga aaaagacacc
     9841 caaggggcag cggctgatgc tcgccaaggt ctctggtcat gtctcttatg ccgatgcaga
     9901 gtcgatggga aatcagctca agcccggcca gccgtttcac caagcgctgg tgctgtgtgt
     9961 cgtcgacaag agcaccgact atcatcccga tgcgcggcgg cacttcaaca cgttcaatgg
    10021 caactacaaa aagctggcca cggtcgtgac cagcgtgctg attcgcgccg cgataaactt
    10081 catgatgcgc gtcagtggcg tcggacagga cgcggtgttg ttcaacaccg aagcagacgc
    10141 gctggcttgg cttgatggct aaacagagtg gaaatcggcc atccgatccc gtgcatccta
    10201 ttccagagaa atcacttcac cgcgcgacgg aatgcgcacg cggtggcctt tttgggtcat
    10261 ttcgtccttg agcggttttt gcgcttccgg ttcgccgtgg accaggaaaa atgttcgtgt
    10321 ctcggttccg cagcgcctgg caaactcgag cagatcgcgt cgatcggcgt gggcactaaa
    10381 cgaattcatc acttcgaccc gcgcgtgtag atcccgttcc acaccccaaa tttttacgcg
    10441 cggacgctgc tcggcaagcc gtcttcccag tgtgtgctgg gccatgaaac ccacgatcag
    10501 gatcgtattg cgttcatctt ccacgttgtt tcgcaggtgg tgcaaaatcc gtcctgcttc
    10561 ggccattccc gacgcggaaa tgatgatcgc tgaccctttc agatcgttga gccgcagcga
    10621 ctcctcccgc gagctgacaa agcgaacttg ggaatagtca aagacctcac catgcgcgct
    10681 gaggaatgcg cgggtttcct gatcaaaaca ctccggatgg agcttaaaca cctcggtgac
    10741 gtttatcgcc agcggtgagt caatgaacac cggaatcggc ggaatccgat gttgggtgct
    10801 cagcgaatgc agcgcgtaga gcagctcttg cgtgcgtccc accgcgaagg ccggaacaat
    10861 cacttttccg ccgcggcgca cggtttcctt tatcacccgt tccagatcat cttccatttg
    10921 cagaatcgat ccgtggacgc gattcccata ggtgctttcc atcaccacat agtcgatggg
    10981 agggggcgga tattccggat cacggatgat gggaagacct ttgcggccca gatcgccgga
    11041 aaacagaatc gttcgtctgg ttccattttc ctcgacggtg agaacggctt ccgatgaacc
    11101 caagatgtgt cccgcattgt aaaattcggc gcgaacaccc ggcagcggat aaaacgtgtg
    11161 gtgatatggc atcccgacaa agcgctcgag cgctttgatg acatcttctt catcgtagat
    11221 gggcgcgatc ggcgaaaagt cgggatcgtc tttgtacttt cgattcaggt atgcagcgtc
    11281 tgcttcctga attctggccg aatcgcgcag catgtatgcg cacagatcgc gcgtcgctgg
    11341 cgtcgcatag atcgatccct gaaagcccga ctttacaagc gtcggcagat tcccagagtg
    11401 atcgatgtgc gcgtgggtca agatcgcaac atcggcttgg gtggcttctt tgggaagacg
    11461 ggcattgcga cgatgtgatt cctcgcgccg tccctgaaaa aaaccgcagt cgatcaggat
    11521 ggttccttgc gcaagctcga gcagatacat cgagcccgtg acttcttccg ctgcaccaag
    11581 aaaatgaatc tgcatgagct gccctcggct tgtcccttac caaaccctct attcatccgc
    11641 tactgaatcg tggagtttgt cacctgcggg gtgccgctgc cgaggaaaaa gatgccttta
    11701 tagcccgcgg tttcaaatcc ctggctggga tttccactga gcagcgagtc tttgatgatg
    11761 agcgatccgg tgcggttgtt gctgacaaaa aagatcgcgc caccgccttc cttcgcggtg
    11821 tttccggtca tttttacgcc gcacagtgtc agcgtaaaca gattcccatc acagtagatt
    11881 gcgccgcccg aaccgccgcc tggcgtgccg ctgcgtggag gatttgcacc aattccgatc
    11941 gcagcgtttt gggcaaacag actgtttaac accgtatagg acacgccgat gctcgacagt
    12001 ccgccgccgt ttgatccgct gtttcccaga cccccttcgc cgccgaacgt actgctgaca
    12061 acataggctg ggagtcccat ggactgctga agcacgcgaa ctgcggcacc accaacatcc
    12121 ggtccggtat cgtcgaccac gttgcggaag aagcgcgagt ttaccacttt gaagcgacca
    12181 ccacttgcga agatcgcccc accgccacct ggactcagtc ctttggcatt cccatccaca
    12241 aatgtcaggt tctgcaccgt aagctgcgga tggtcctgat cttggcagtg cgtggtcgtg
    12301 tacttctgcg cggcgtcaca ggtgttttga tacagaatcc ggactttgcc gccgccggac
    12361 agtgtgactt tgccaccccc atcgatgaca atcttgggtc cgcggtcgtt aaagattttc
    12421 gcggtctggg tcatggtgat ggtgatcgga tcgggtccgc agttgaacgt gatgatgccg
    12481 cctttggcga ccgcagcgac gactgcatcg gaagtacagc tctgcgcggt gccattgcca
    12541 atcacggtcg tcggcgctgt ggagacttct gcttgggctt ccgtgggaac cgcacatttt
    12601 ccgtccggat ttcccgccac cggcaaggcc gtcggcagcg ggcccagatc cgcgcttggc
    12661 tccggtccca aatccatcgt ctgcacactg cttgattcac agcccgcgct gtgaaccagc
    12721 aagacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaca cccaacgcaa
    12841 cgtttgcaca aacgaaagga cgcgattgca acgacatcat cacccccgga aggagtgtag
    12901 caagccgggg actacttcca ccaccgcgtc gtggatgtcg tcgtgatccg tgtgagtttg
    12961 ctgcctatcg ggatgtcttc gacaatcagt tcgttaaagc gcggtcgcgc gaaaaccgca
    13021 ggctgatcga tctggatgtg gatcgcgcgc tgcgattcct gacccgcagg gagggccgga
    13081 cccggctcct tttcgatcgg tagacgccgt ccgttccagc ggacggacag cgcactgcat
    13141 gatccgggga agctgccttc cagggtcagc cgcagtggtt ccgacaccac cagaggaatc
    13201 agtacacgga tgcgtgactt tacattgcct tcgcccgcaa tgcgggaaag agacagtcct
    13261 tcgaccactt gcggcgggtt ttcagcgacg acaatgctgg gtgtatgaac gacttcacgg
    13321 atgcgccagt cccgttcatt gacataacta cccagcaagt cttccacccg cacaagcgga
    13381 atctgatagg ccagcgcata ccaagcagac accggttgca aaaacggata gcccgtccgt
    13441 tctaacagac gcgccaggaa ttctggacct ttggcccagc ggactctggt ggctgcggaa
    13501 aaggctccgg cactggacga cttgttgcga ccttggcgaa ccagctccac catcatcaga
    13561 ttgaagctgg caaacagcac aagaagtccc cacaacagcc gggacacggg ctgtttctgt
    13621 ttgggaatcc gatccaaaag ccagacaaac agcgcgccca gaccgacggc aaataccacg
    13681 acggcatcgg tgtagcgccg cggaccaaat gaccagctac cgtgaaaatc ccacgcgctc
    13741 gcgttcacat acagttcaag aatcgccgtc aaaaaaagcg gcaaagtcag cgcgcgatgg
    13801 cgacgcagcg caatcagtag accgcaagca gacagatagg tcagcggaac ccacggaaag
    13861 attccggccc gcagcgaaaa cagcgactcg acgacagccg gggtatccca cagaaagtga
    13921 ccgggctttt gcggtgcgcg cagaacgcca tagtagtgca accacagaag cacctgcggc
    13981 acaaacagaa gcgctgccac cgacatgatg aggattcctt ttccgatgat cgcaagcgcc
    14041 cgctttccgt ttcgcatccg cagatcggac acgacaaggc ccagcatcca cagcgcaagc
    14101 ggcagcatcc acagcgcttc ttgcggacgc tggagcgcgg cgattccgga ccaaaaaccc
    14161 agcaccaagc agcgacggac agacagatca tgtcggtagc gatcacttag gtccaccagc
    14221 atcgtcacgg cgaaaaatgc ggtcgcatgt tggtacagcg gctgggtggt cagataccaa
    14281 aacagcggcg ttgcaagcac tgccgaacag gtcgcaaagc gcgctgccgt ttggccaaag
    14341 tgacggcaca gaaaatcgaa cagaatccgg attccccaca gaccggcaaa cagactccca
    14401 agtccggcca tgtacagatc cgcttcggtc tgtccggaca gaacctgcgc cggttttagc
    14461 gtcggaacaa gggccgacaa aaggattccc aaacccggaa caagcgccag tttttgcaaa
    14521 aggagtccaa gcagataaaa cggaaaccag aacagaagcg ggcctatcgg acagggattg
    14581 gcgacgagac ctgtcgtttc cctgccaatg atggcggtcc actgtggtgc cgcttgggcc
    14641 agatcaaagc tgtgatgaaa cacaagtccg ggtagatact gaaaaccgaa aaatccgtcg
    14701 cccgatcctt tgccctggaa aattccgcgc gtaaatcggt agtgcaaaat tgcgatgatc
    14761 cacaccgccc acaccgatcc atcgattgag ctggcagctc ggcgcagtcg ggcccacaga
    14821 gcgtgaactc tgttttcggt cggggctttt tcggaatcgg aattcacgac gaagtatccg
    14881 cgaaagagct tggcgaaaag acatcggctc tgtcaatcga atctgggttg accgtgacgg
    14941 ggattccttg ctacacatcc tggccgtgac tgcacctgct tcttcgtctg aatcgaccct
    15001 tggtagcaac ctctttgccg cacgctggcc gggacgtgat cggcgtccag ttcgtgttcg
    15061 cattgcgcct tctccgacgg gagatccgca cgttgggact gcgtacattg cgctgttcaa
    15121 ctacgtgttt gcgaaaaaac acggtggcca gttcatcctc cggattgaag acaccgacca
    15181 gacacgatcg acgctggcga gcgaagatgc gatcttgcgc gcgctgcgct ggatcggact
    15241 caagtgggat gaaggtccgg actgcggggg tccgtttggc ccctatcggc aatcggaacg
    15301 gaccgatatc tatcagcagc atgcgcagat gctcgtcgac aaaggcgcgg cttatccgtg
    15361 tttttgtacg tcggacaggc tggaagcgtc gcgcaaggcg cggatggcag aaggcaagcc
    15421 gcccggatac gatcggctgt gtcgctatct gtcgaaagaa gaagtcgcag cgcgcaaagc
    15481 ggcgggtgac aaatacgtca tccggctcgc gatgccgctg gaaggggaga cccgttttta
    15541 cgacggactg cgcggcgaca tcgtgatcga caacgcgaca attgacgatc aggtgctgct
    15601 caaatccgat ggatttccga cctatcacct ggccaacgtc gtcgacgatc acctgatgca
    15661 agtcagccat gtgatccgcg cagaggaatg gatcatcagc acgccgaaac atgtgcagtt
    15721 gtacgcggcg tttggctggg agccaccgga ttttcaacac atgccgcttc tgcgcaatcc
    15781 cgatcgcagc aagatcagca agcgcaaaaa tccggtgtcg ctcgactact atcgcgatgc
    15841 gggtttcttt ccggaagcgc tgctgaactt cctttcgctg atgggttttg cattcggtgg
    15901 cgatcgcgaa aagttcacgc tggccgagat gatcgaagcg tttgactgga ccaaggtttc
    15961 ggcaggcgag ccggtgttcg atctgcaaaa actgacctgg ttaaacggtc tgtatctgcg
    16021 cgagatgccg cccgagacgc tgcttgaacg gctgcgggcg tggcgtcttt cagacagcta
    16081 tctgctttcg ctgattccgc ttctgcgcga acggattgag cggatggatc agttcatccc
    16141 gtcggccagc ttctttctga ccggcgatct ggattactcg gcagtgcttt cccagctgat
    16201 tcccaaaggc cgcgatcagc tccaggtcag ccagattctg tctgagctca cagactatct
    16261 ggaagaacat gtacgcaccg gctggaccca ggaacttttg gacaaagcgt gtcgcgattt
    16321 ttgcgaaaag agcggctgga aacccaaaga gctgttcatg ccgattcgca tcgcagtgac
    16381 gggacgcacg gcatcgccgg ggctgtttga aacgatggtc gcgattggca aagacctcac
    16441 gcggcggcga ctgcggctcg catcgcaagc tgtgctgact tattccgcac ccgcggtggc
    16501 cagtccggct gcacagcaga agccgagccc acagggcaaa ccagcgcccg caccaacgac
    16561 gaaacccgcg taaggacaag gccaaaggaa tgctgcgaag gattcctctg cttgggtttt
    16621 tgctctctgc gtgggattcc tttgatcagg cggcgctgca gcatcgcagc acgctgtccg
    16681 ctgagcaaaa tgcccaggaa gtgaagcgga ttcgcgagct gttgctcgtc gcgggtctgc
    16741 ttctgacgtt gggctattgc ttcggtgatc gaccgttttt tgaagaagta ttcgggccgt
    16801 tcatcagtcg tcatccgcgg cttgagcact atcatgagct gctcagcttc acgtactgga
    16861 gcgtggccaa gctgcttgga tatggggtat ttccagtgct tcatattgtg ctgcgccgcg
    16921 aatccccggc ggacttcggt ctgtcgttgc gcggcgtctc tgccgtcgaa gaaaagatca
    16981 gactgccctg ggcgcggatg tatcttgtgc tgttggcgtt gatcttgccg gcggtgtttg
    17041 cggtcagctt cactgcgagc tttcagcaaa actatccctt ttacggaaaa gccagccgca
    17101 gtctgtttga tttcgtggtg tgggaaaccg aatacctgtc cacgtttgtc gcggtcgagt
    17161 tctttttccg cggctacctg ctgttcggtc tgcgacggta tttgggaagt ctggcgctgt
    17221 ttgtgtcgat ggttccctac tgtctgatcc acgtactaaa gccagccccc gaagccatcg
    17281 gatcgatctt tgcgggactg cttttgggaa cgctggcgct gtgtaccgga acgctgtggt
    17341 ccggtgtgct gttgcatgtc tcggtggcgc tgtcgatgga cgtgctgtcg tcgctgcaat
    17401 cgggtcggct gccgaagttt tagcgactac ttctttttgt cggccacgat ctcttgcaga
    17461 cccttgatcg cgttgatcga atcgttggca aacttcatcc agtccttggc ctgttcacca
    17521 acgtcgccca tcgacgccag atccgtcagc gcggtggatg ccagatccat tccgatggaa
    17581 cccgcgctct gaatcgacgc gcgcacgttc gtcggatcga cgccccacgg cagctcaagc
    17641 agcggcacaa agtacttggt caggacgccc gccactttgt cctctttgac tttgcccttg
    17701 aggatgtcga gaagctggcc gcactcatcg acgctctttt ttcccgtctc gaaacgctgc
    17761 gtcaaggtgc cgatttcatc aaggaacttg gtcgtcgctt cggcaatcgc tttgcgtttc
    17821 ttttcgttgc tctttttgag cgcgggattt gacgcttccg cttccttcac ttctttggaa
    17881 tcgagcgcct tgagcgtcgc gtccgacttg ctgaccacat cggcgacttt tttgcccagg
    17941 tctttggcgt tgaccagcag gcccgacacc ttgccgttgt aggtcttgag ttccttttca
    18001 atgtcgctgg ccgacttgac cgacacgccg ccgagcgcga gtacttcttt gagcgcgggc
    18061 ccaccgggaa ctttgcccag cacttctttg ccaaagtttt tccagcccgt cgcggactgc
    18121 gattcgatcg ccaccgccag cactttggtc aggtctttgc ccattttctc gacgttgcgg
    18181 aatgcttgat acgccgccac cgccgtttcg atgccgcctt tgatcgtacc caccagaccg
    18241 agcaccgctc cgacgccggt gatgccaccc accgccgcgg tcgtaatgcc cagcgtcagc
    18301 gagatcacgc ctttgccgat ggtgatggcg gtatcgacct tgaagtcgcg atagtcgaca
    18361 ttggcttcaa gctgccgcag ccactgatcc gtgaccgact tgacgattgc ggtttctgcg
    18421 tcggaacgga acttgtcgag ccgatcgttc gcttttttga ccgacttcgc tttcttcgga
    18481 tctttgtcgt ttggcagctc ggcatcgatc gtcttcagat cgttcgcgag cgcctgcacg
    18541 agcggcttaa acacctccgc cacttggtcg cgcaaaaacg ccgccgtgtg ctcgttgtct
    18601 ttggccttgg cgaccatctt gccggcgatg ttcaccgaca ccatcagctt ttcgggcagc
    18661 gtcaggccgt tggccagcac ccattccttg ctgtcgtcgt cctgctcgcc cttgagcggc
    18721 gctttgtcaa acagtgtgtt gtttagaaac aggctgcgcg cgacatccca ctgcgcgatt
    18781 tcccagactt gatcgccaag cgagcgctgc atcagcgcct tgccgttcac cgtcagcgcg
    18841 ttcaggtctt caccgatcag gttccacagc gacaccgaaa acatcgggtc tttcatgtac
    18901 tcggcgagca ccgctttgct gctgtcgtcc gccgtcagat cataggtctt ctttttcttg
    18961 tcagcgacga agcttccctt gatgctcttt tgcggcttga ccagcgactt ccagtagcct
    19021 ttttcgtcgg cttctcccgt ccacgaaatc accatcaccg gctgcttgtc gcccgccgcg
    19081 gacattgtga acgaaaaggt gcccttctct ttgaaaacgt tcagctttgt ttgctgctca
    19141 tcacgcaatt gtgtgagatt ctttgggtct ccactaaata gctttcgcgc tgtatagaaa
    19201 ttgataagtc ccattccatt ctcctttgat ttcgctgata tcggccagcc gttcttgggg
    19261 caccgaggat cgggcttgtt cgacggaaaa ttcccacttg gttacgttcg cctaccaggc
    19321 ggtaactatc tttatctctg aaataatcag acattgtcaa tgcatgcgta tttgtttgtg
    19381 tgtaatttat cgcaatggtt tgtattaaaa gataaggttt tcggtgggtt atccgaagcc
    19441 gacaaatgtg tgcgtggggt ggggcggtag ggaatcccca tgctgggcag gggcaccgtc
    19501 ggggcatagg tatcggaatg gacagggaaa gcgggcgatg cgtcgtgtga cgacggcgcg
    19561 gaacaccacg caatcggcgc ggatctgacg atggggatgg gaatcggcga gggcgggtga
    19621 acggggaatc agacggaggc ttgggcgact tcgcgcagct cttggccgtg gacaaactgt
    19681 tcgatgagcc gggcgacccg tggcggcatc cgcaaaaagc gaatcgccat cgatcgtgcc
    19741 gagcgccccg ctgtgagcac cacgccttcg atgcagagca cctcgggatt gcccggaagt
    19801 tcgagttcga tcgtgacgga cagatggggc acgtcgggtt caaacagttt gtgaaccagc
    19861 atgccgccgc ggctcaggtt cacgccgcga cagacgtggg gttggccgtc gatgtacttg
    19921 ttcaggagaa tgtcgatcgg tctgcgcggg ttgtgccgct tttcgctcgt catggtcgct
    19981 cccaaaagca tgaaatctcg tacatcagac ccatatgtag ggtggatgta ggatacgacc
    20041 acaccgcggc agatcaactt tttccgtttt tacccatccg agcagtcgag aaaaaaacca
    20101 cagcggggac agcggagctt gcagccgcga tcaaacagct cgctgctaca gccgggacag
    20161 aagcggtggc tttcgatttg aagccggtcc gggtcgggtc cgtggacagc cggaccgtcg
    20221 gttgttgcgg tcgtgcgcgg ctcgtcggac agcgatacag agggtgcggc aatctggcgc
    20281 attgtctttc ctcgcgacga gaaattagcg cttttcggca attgccacca tcgggaaagt
    20341 cgctgctgat aagatggatt gctaagcctg tctccgatcg cagtcggagg cccagccttg
    20401 ggcagcccag gccagttgaa aagatgcgaa gtcgccacat tcacagtcaa gcgcggtggg
    20461 gtaagcagtg attttaagtg aatctgcacg ctcgctgctg gcgctgtccg tcgatctccg
    20521 cgacgctgtg gtgttgctgg tcgatgactc cccgcgtgac gccgaagagc tcttggtggc
    20581 gctcaccgag gtcgggctca aagtgctcgt cgcggaaagt ggtcccgcgg ccaagcagaa
    20641 gctgatgcgc gaggcggtcg acttggtgat cgtcgatctg aagcgcgccg acatggacag
    20701 cttcgagctg actcgccagc tgaaagtgcc gccgcagacg cggcttttgc cggtgctgtt
    20761 tgtgtcggcg aaaaaagaca tcgactcgtc gattcgggct tttcgcgtcg gtgcgtcgga
    20821 ctacatcgac accgcgatcg atttttccga gctgctggtt cgcatcgagt ttcacattcg
    20881 ccatggccgc aagctgcgcg agctggagcg tgaaaaagcc gagcttgccc gtgagctgcg
    20941 cgagctaaaa aaagaaaccg cgaaacagat tgcgcaaatt ccggtctata caaacctcgc
    21001 cgagctgccg agtggctttg tcatcgacgg aaaataccga ctggaaagcc tgatcggaac
    21061 cggcggattt ggcgtggtct accgcgcgac gcatacccag cttttgcgtc aggtggcggt
    21121 caaagtgttt cgtccgctca ccaacctgag caatgaagat gcgctgcgtc gttttcgtca
    21181 cgaaggcgcg tcggcgagtc ggcttcagca tcccaacgcg gtgacgatcc tcgattcggg
    21241 ggtcgcgccg ggcgggattc cgtatttggc gatggagctg ctggttggcc gaacgctcgc
    21301 cgacgagctg aaggaaaaac gggtgctgtc gctggcgcgg accgtgcaga tcatcctgcc
    21361 ggtctgcgac gtgctgatcg aagcacacgc agccacgctg gttcatcgcg
//
LOCUS       DAORGN010000073        21255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_983_length_21255_cov_6.675425, whole genome shotgun sequence.
ACCESSION   DAORGN010000073 DAORGN010000000
VERSION     DAORGN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_21255_cov_6.675425"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKL17_10035"
     CDS             complement(<1..317)
                     /locus_tag="PKL17_10035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNK45112.1"
                     /translation="MIDSILMFLAEHTAVAALLKLIVLVLGFVMPLASLSTWAERRQS
                     AMMQDRLGPNRANIGPIKLWGILHFVADAIKMMFKEDVVPAKVDRFLFALAPVMAIAP
                     VLIT"
     gene            complement(329..2137)
                     /locus_tag="PKL17_10040"
     CDS             complement(329..2137)
                     /locus_tag="PKL17_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNK45113.1"
                     /translation="MAETPSSPIKSSIAAALRETASPPPPPAAAPPAPASTAPAGPAI
                     SAPPPSELCTIKLDGREVQAKKGANLLEVCLQHGVDVSYFCYHPGLSSPAVCRQCLVE
                     VVGQPKLVPACFTPVTDKMEVNTATPRVLTIRRQILEFTLVNHPVDCPICDKAGECTL
                     QKQYQDWDHHGSRSSIPKVHKPKMQDIGPHIVLDAERCILCTRCIRVCDEVAGEKQLT
                     MAWRGDREQLTVAPGHQLDNPYSLNTVDVCPVGALTSKDFRFTMRAWELFTTSSVCPG
                     CATGCNVEVHHAQGEIWRLVPRENLQVNKYWMCDEGRFTYKDVRHKRLTSARLGAGRG
                     QPAQITSVDKAIAFAAERLDRLASQGGTIGLVFSAQTTNEPLFALSQIAAKLAARGAS
                     VRTYVTGVAASPERADKILRSADKNPNTAGAKLLAGTGAGDANALLSDLKAGKLKALW
                     MVGADLPLSEQAQFDVAPLVDGLDLFICQAVQDSPILRRAHVQLPAAAWAEIDGTFTN
                     AKNLVQRLRKAVAPQGDARSHLELISLIAKKANLGQNEKWQVSARQAFLQMVAQFAAQ
                     VPAGADPAQNVAAGFATAEWGKEYLPVQLRFANSRG"
     gene            complement(2158..3510)
                     /gene="nuoD"
                     /locus_tag="PKL17_10045"
     CDS             complement(2158..3510)
                     /gene="nuoD"
                     /locus_tag="PKL17_10045"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNK45114.1"
                     /translation="MSTTTNQSKTGPKKLAFGMPTPDEIAAGGIPGLSARPKVPQDLM
                     DKLNTEELEEVEDIELPTEPMTINMGPSHPATHGTVRIILTIDGETVKDSDVQVGYLH
                     RCFEKEAEAATYTQVFPYTDRLNYVSPLLNNVGFALAVEKLIGIVDQLPERAKYIRVI
                     IGEVSRITDHLTCLGASAMEVGGFTPFLWMIKVREWLWELLEELTGARLTHSYVRIGG
                     VSQDLPEHWLDKLRFTLDKSQEALGETESLLVKNRIFRDRMDGVAVISAEDAISYGWT
                     GPCLRSTGVPYDVRKDHPYLVYDRFEFDVPVGSVGDNYDRFAVRLEEIRQSMRILDQA
                     MKQIPPGPFLLDDPRVCMPPKNAVYNTIESMIGHFKLVIDGIKVPPGEVYSYTEGGNG
                     ELGFYIVSDGSGRPYRCHVRSPCFIIMQSLSKLIQGAGIADIIPTFGMINMIGGECDR
                     "
     gene            complement(3525..4019)
                     /locus_tag="PKL17_10050"
     CDS             complement(3525..4019)
                     /locus_tag="PKL17_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNK45115.1"
                     /translation="MSKADLDKLVAQFPAAIVSSHSSHGDDTAVVSKDSFKDVMRFVR
                     DTAGFDFLVNVTAVDYFGREPRFEVVYHLRSMATGNRLRIKVGLHEEEGGPSAEVPTV
                     SDLWQTANWQEREVYDMFGVKFTGHPDLRRILMYEEFVGHPLRKDYPKEKRQPLIRRD
                     YSLS"
     gene            complement(4065..4592)
                     /gene="nuoB"
                     /locus_tag="PKL17_10055"
     CDS             complement(4065..4592)
                     /gene="nuoB"
                     /locus_tag="PKL17_10055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNK45116.1"
                     /translation="MAESYTTRLDDALNWARKYSLFSYPFVTACCGMEYMATAASKYD
                     IARFGAEFPRFSPRQADLLFVVGTITERQAPVLHRIWMQMAEPKYVIAFGVCASTGGF
                     YQNYNTVPGCDQIVPVDVYIPGCPPRPEQVLDGLILLQKKIQEATTWVQRRDRKEVEL
                     QAASTDGRKRLPVVA"
     gene            complement(4614..5027)
                     /locus_tag="PKL17_10060"
     CDS             complement(4614..5027)
                     /locus_tag="PKL17_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNK45117.1"
                     /translation="MSVEQATDYGPTLVAILFGIAFSCVFLALAMFLGPKRENAEKLA
                     PFECGSEPIGSPRVRFSVKFYQVALLFLIFDIEAAFLYPWAAIYRRLSCLGTVIHSEC
                     VGTVTSFGFVEMLVFMSTLVVALAYVWRKRAIGWG"
     gene            complement(5124..6149)
                     /locus_tag="PKL17_10065"
     CDS             complement(5124..6149)
                     /locus_tag="PKL17_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45118.1"
                     /translation="MKRRTWAVWLGMLLCLGVCVGCKRPEPTPATLPMRVVDIQIASQ
                     TDGSAAQQKAPTELDRDMLLAAAIEGLRSADVAVALAPAEREPGDFVLQIELGLVYSA
                     GTESKPKPPLLRALTAGLLRTRRGGDAMSDEKTPQPELTRLQHLAVAEQSGKTTSPTG
                     LVWSDLARRAVRDTSHSLGAQLRLCGVKTSELTKLANDNSKEPDLRGVALRLLAMRKD
                     QGAQKLVMAVLKDRQSPSALRDQAIGALVELGDPKSVRPLLDSTEFRDRSELGKVLEA
                     AAALGGDEAQRYLEFVAQSHSDAQIRDEAKTALGHLLSAKARQESAKAAAKDNVGGPT
                     REAGTPD"
     gene            6342..7295
                     /locus_tag="PKL17_10070"
     CDS             6342..7295
                     /locus_tag="PKL17_10070"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNK45119.1"
                     /translation="MQKKPQRPVPSVEIIDTPRVRPPRPIQIDKLTSIPFFLVHIAAL
                     ASAIYLGATKTDIIVCVALYYIRMFGITAGYHRYFSHRAYKTSRFFQFVLAVIGGASM
                     QKGVLWWAAHHRHHHKWSDMSEDIHSVKQDGFYWSHVGWIISNEYDETEWDRIKDFSK
                     YPELRWLNRWHWAPALIGGAILYAIGGIHWFVWGGLVSSVFLWHGTFFINSLAHVWGS
                     RRYPTTDTSRNNFWLALITMGEGWHNNHHYYQSTANQGFFWWEVDFSYYILKMLSWVG
                     VVWDLRTPPRHILEGKAAVDPIEPEVATEVPALPPAAVPSV"
     gene            7349..8053
                     /locus_tag="PKL17_10075"
     CDS             7349..8053
                     /locus_tag="PKL17_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45120.1"
                     /translation="MSQNNATDWQPAQPGEGRSPCPALNSLANGGHLPRNGIASRSQL
                     ITAIVGQLGISTEFANTLANAALEKFGKPGDGGEQVLHLQDLCQHGKLEHDASLTRQD
                     AHAGDHAKVDPSLVEQLLSLSKNGQTLTLDDLAAAHQIRMHQSAQGGHHVPGKAGFVG
                     TVEAALLYTVLARGESGISLADAREFLLTERVPQGLAGRNITLAQVAVRAIAIAAKGN
                     LPVCEAARRAKEATKK"
     gene            8109..9314
                     /locus_tag="PKL17_10080"
     CDS             8109..9314
                     /locus_tag="PKL17_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45121.1"
                     /translation="MPFLELLLTALDTLRANKARAVLTTLGVIIGVLSVILLVSLGEG
                     ARSYLGDTFAGLGSNLLQVQPGRRETKGFGGPSLGTVYKITREDEHMLARRATTADGV
                     AGVVTGGGTVRYLNRRRDTLVLGVGSRFIEIRQMNVAQGRFFTDEDIESRRRFVVLGR
                     TVLQELFGDENALGKVIKVADGEFRVIGIMEHKGQTLGFDLDDIVFIPETTGLDLFAL
                     DGLSHILVRARDKISVDPAIDEITDLLRRRHNDQVDFTIISQDDMLSTVNGIMNTFSM
                     VLVAIASISLVVGGIGIANIMLVSVRERTREIGVRRAVGAKKRHVLMQFLLESVVISL
                     LGGLIGLGLGALIIMVGRLLLPGLPVRLSFDIVLIAVGFSGMVGVLSGVVPAQRAASL
                     DPVEALRYE"
     gene            9322..10545
                     /locus_tag="PKL17_10085"
     CDS             9322..10545
                     /locus_tag="PKL17_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK45122.1"
                     /translation="MSSENTSAKSDAAPTEKPRWLSKVVFGFGIASLLSDAGHEAGTA
                     ALPVLLTTMAAAPATLGIIEGIADGVVCFAKLFGGSLANRPQWRKPLAVTGYLVTGLS
                     IGLFALSTNWLHILGSRILGWLFRGLRSPSRSAMLADAVPKTALGRAIGFHRAMDTVG
                     AIFGPLLATLLLLRLPLRQVFWVAMVPGVLAAVAFLLLVPGQTTTSREPPPSLLRGMK
                     ELPPAFRRFLIAVMSFGIGDFARTLLVLRATQLLPAQDIPGGAVSVAILLFALHHAVA
                     AACAFPVGYLADKMSPRWLLAVGYGLGVVTALLAAFAQPSVVYLAGLFAVAGMVIGIE
                     ETIEGVLCARLALPKLRGTAYGMLAATNGIGDMVASSVVGVVWAAYSPQLAFLLAAVA
                     CAVGTVLLVVAVPDE"
     gene            complement(10554..10859)
                     /locus_tag="PKL17_10090"
     CDS             complement(10554..10859)
                     /locus_tag="PKL17_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNK45123.1"
                     /translation="MSQGATQKPGSKPDALPDSAQRLHARREKHDREQHGLISTAVTM
                     VGLGWLVVIPGLFGIYIGKWLDGKVGQGVTFAAGLGLLGVCVGCLLAWQRISANRPK"
     gene            complement(10856..11347)
                     /locus_tag="PKL17_10095"
     CDS             complement(10856..11347)
                     /locus_tag="PKL17_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNK45124.1"
                     /translation="MADASLELEPSQRSLRLTIVTPLQLVLDLDGVRHVRAEDPTGSF
                     GILPGHADLLTVLSVSVLIYRAGGGPDDPAVEHFVGLRGGVLFVSGGRHVQVFTREAT
                     PSDDLRALSAEVKSRLSLAVAQESEAYRSFARMEGALLSHMTDYLRHEHRTNGPRRLL
                     SEQ"
     gene            complement(11360..11890)
                     /locus_tag="PKL17_10100"
     CDS             complement(11360..11890)
                     /locus_tag="PKL17_10100"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNK45125.1"
                     /translation="MVSDPALALLARHIRDVPDFPKPGILFKDITPLLADATAFRLVL
                     HLLAERLAPLKPTMIVGVESRGFIFAAPVAERLGLGFAPVRKPGKLPYRRVTETYDLE
                     YGSGTLEMHEDAVAGQRVVIIDDVLATGGTAAGSARLCQKQGAEVVGYGFVLELSFLH
                     GRKALGGHPAVTLLSY"
     gene            complement(11933..13228)
                     /locus_tag="PKL17_10105"
     CDS             complement(11933..13228)
                     /locus_tag="PKL17_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK45126.1"
                     /translation="MSEAMTEKTSPKRKIWIWLLAVVAVVAIGFGAYRLFFHKSEGPR
                     WVTAKVTRGDVTQVVNATGTIQPLLLSPVGSQVSGIVHKLHADFNDTVKQGQVLVELD
                     PALFQTAVKSQEANVASAQANLIRAKADALNASRIAERSRALFAKSYISAADRDQSVA
                     TEQSTAASVQAAEAGVKLAQAALDKARLDLKNSVILSPVDGIVISRNVELGQAVVASF
                     QAPNLFQIAGDLKKMQVLANVDEADIGYLRVGAVAEFTVDSFRGKRFAAKVSQIRNAA
                     QTVQNVVTYVVVLDVDNGELLLRPGMTANVRVVVAERKDVLLVPNAALRFKPRLDRAP
                     GENKGEGRERKREGRGEGRNKSPIFVPDGERFHPVFVSAGISDGLFTEVSDGLTEGQA
                     IIVDSAKSAGGGSGANPMGGPRSGGGGGPPGGGMRRVGM"
     gene            complement(13225..14667)
                     /locus_tag="PKL17_10110"
     CDS             complement(13225..14667)
                     /locus_tag="PKL17_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK45127.1"
                     /translation="MNTRAWLCTGALSMLLTAPVMATEPSADTAPTVSSLSGPPLPLV
                     EAIQTALVHNPSAEVARQTRLSSEAKVGTARSPWLPQLSLSGSVRGDYSYQIGAPEGK
                     SSVSTFRSSGVLSLNQLVWDFGRLSGRIAAAQASAEASLTDEKNARAQVVLSTMSSFY
                     AVLQAEALLSVASENLQQQTRRLKQSESFFKIGTKPQIDVLIAQTAVAQAKLQVLQAQ
                     GNVQVTRTQFLQALGLPERDWGSWHKRPLQSTLPAPHPIEAALSNAVSETVATVPDEV
                     VERTLQARPDYQSLLARVRQSEAQLKAAKSDYLPSLSLGASATMNSGTFGGVSGLDVT
                     TQALPGLLVSGTVGLTWPVFTGLSTVYAVRDARAQLALAEANLTQLRLQVRSQLQQAL
                     QQVLTAWLTVEAAVAVANQAKKQLEMADGRYRAGVGNAIELGDAQVAAMQAQAQRVSA
                     DFALAQQRASLRFYLGELIKETEPRGDATP"
     gene            complement(14667..15779)
                     /locus_tag="PKL17_10115"
     CDS             complement(14667..15779)
                     /locus_tag="PKL17_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNK45128.1"
                     /translation="MLPPPLTALLKRVSRSFFLSVRVLPDEVAAPIGLGYLLARAADT
                     IADTDLLPATTRLSLLGELSSASEGDVAALARLQLALRELLRQPMGQALPSSEQTLLA
                     VLDECLSLHLQADEPDRRLVTRVLGQLVSGMQTDLRRFPAAGSVSSADQVVVLQSLAE
                     LDEYTYYAAGCVGEFWTELCAAHLPGLAHLRSPELVDCGVSLGKALQLTNVVRDLAAD
                     LRIGRCYIPQPLLDEHGLTCARLYDLTRTPPRDLGEARAMRKLTATLLRLGIRRCDEA
                     WPYVLAIPKSLVRLRLACVWPLFLALDTLAMLGNVGSPLVTPDQPVKVSRQSVYGLVF
                     ATTLSTLAADVGASDRFLERQFQHKRQLAWRSVEGS"
     gene            complement(15791..16459)
                     /locus_tag="PKL17_10120"
     CDS             complement(15791..16459)
                     /locus_tag="PKL17_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45129.1"
                     /translation="MLLASLVPGCDKGLAGLLDNSGVGATLLPGVKLNLPPPPSFAEP
                     NIPREYPDGTVSIYGLRKEFKRYSDTKNKYLGQKVKVKAYLLEVYKCPVCPKGQQCKQ
                     CEEPHMYASDEANGSKEKGLLIAEYRGFRGREPKLTIGKQYVFEGVFNQSSPKGFTSS
                     DGLLVYHKMLDDTNKEHIGPAEENERQAALAEAAALKAMGQRPPTPPTPAPGAVKPPT
                     PPAK"
     gene            complement(16646..17872)
                     /locus_tag="PKL17_10125"
     CDS             complement(16646..17872)
                     /locus_tag="PKL17_10125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK45130.1"
                     /translation="MHFGIVSTSYPRFAGDPAGGFVHGLNRHLHRLGHRVSVVCAGDS
                     DAAVFERLDGIAVHRLPSSLFFRGGAPDALVQGLRRKPIGTLGLAMQFSVDLVRAVAQ
                     FLSVCDALISHWLVPCGLVAGLVSRGRPHVAIAHSSDVHLLRRLGLANMGRWFAARAD
                     LVYTAEHLVIDGAPGRVVPMGIDVAEFALRDGERQAIRHKLGLYRPTLLCLGRLVPVK
                     GVSVLLSALRRLRHLDIELIVAGDGPLRSPLEAEAHAQGLPVRFVGEVLGRDKRQWLA
                     AADLLVLPSLQLPDGRTEGAPVVIWEALASGLPVVASAVGGTTAQLGGCGLAVPPGDA
                     QALAQAIETLLGDPMLAARLRAHGPKQAAQADWAQVARRLLPRWLDVPAAAEVDSGPA
                     VVLTNQHPNCYRERYK"
     gene            complement(17857..18621)
                     /locus_tag="PKL17_10130"
     CDS             complement(17857..18621)
                     /locus_tag="PKL17_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK45131.1"
                     /translation="MYKGLRVAVVVPAYNEADHLPSTLATIPTYVDHVLVVDDGSVDE
                     TSSVAACHARPGVEVLRHLQNRGVGAAVATGYRRALLLNSDVAVVMAGDGQMDPADVP
                     SLIEPIVDGRADYVKGNRFAHREVWQTMPRLRLVGNVVLSLLTRITAGYPRMFDSQCG
                     FTAATRRALLSIDLDGLFPRYGYPNDLLARLWVGRVRVQDVPVRTIYGRTWRSGISLR
                     TAIYPVLFVALRALPWRLWARWRRRQTRVVDPCTSE"
     gene            complement(18682..19731)
                     /locus_tag="PKL17_10135"
     CDS             complement(18682..19731)
                     /locus_tag="PKL17_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNK45132.1"
                     /translation="MKAASRSLLLVVTLVLLGINALLLWPLRADSTAVKDSGRRPLRV
                     GLVFDVGGRGDKSFNDAAFRGVDQAVRELGILAEFVEPGDGADRESGLRLLAARGFDL
                     VLGVGFVFSDDMLQIAHDYPDRHFACIDYAKFDEHGFVVPPRNMVALKFREEEGSFLV
                     GAVGALLSPHKTLGFVGGMDIPLIHKFESGYRQGALAVCPDCKVLVGYAGTSADAFKN
                     PARGKELALAQYQAGADVVFHAAGSTGLGVFEAAREKNRRAIGVDSDQWDDAPGYMLT
                     SMVKRIEVSVYNVIAELREGRFDPGVKVFGLREGGIDYAYDEPRRGMIPTHVRARVEA
                     LRKDIIDGKIVVGDK"
     gene            19791..20552
                     /locus_tag="PKL17_10140"
     CDS             19791..20552
                     /locus_tag="PKL17_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNK45133.1"
                     /translation="MNGSENQFYFGLLIAYWVLGGLTAINLMFRSAPYGRYARKGWGP
                     DIGATAGWIIMEAPASLAPLALFLLSDRRDTVLWVFTLIWQLHYFHRAFVFPFRRRGG
                     GTMPLAIALTAVVFNLGNAYLNWRFLAQLGPVYDPNWLTDPRFVVGTAVFLVGFVINQ
                     HADYVLLHLRKPGETGYKIPYGGMYRFVSCPNYLGELVEWIGWAILTWSLGGLTFAFW
                     TAANLVPRALSHHRDYHKRFPEYPADRKAVIPFLL"
     gene            complement(20715..>21255)
                     /locus_tag="PKL17_10145"
     CDS             complement(20715..>21255)
                     /locus_tag="PKL17_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNK45134.1"
                     /translation="HISDMSWTQRIKHPSEMFQKGDEVEAVVINIDTTDGEKPKISLG
                     IKQLNPDPWNIIPDKYKVGSIVPAKVQKVLDFGAFVELDKGVEGLVHVSEISDDRVED
                     PRTVLKPGQEVNVEVISLDPQERKIGLSIKAANRAAAVSAAQGYGGSGELGAKLGDVL
                     KKKGKKQRRKQDEDEEDEE"
BASE COUNT         4076 a         6899 c         6226 g         4054 t
ORIGIN      
        1 gtgatcagca ccggcgcaat cgccatcacc ggcgcgagcg caaacaggaa gcggtcgact
       61 ttcgccggaa ccacgtcctc tttgaacatc atcttgatgg cgtcagcgac gaagtggagg
      121 ataccccaca gcttgatcgg gccaatgttg gcgcggtttg gacccaagcg gtcctgcatc
      181 atcgccgact gccgccgctc ggcccaggtg ctcagcgaag ccagcggcat cacaaagccg
      241 agtacgagca caatcagctt gagaagcgcc gcgacggcgg tgtgctcggc caaaaacatc
      301 aggatcgaat caatcatcgc atcccctctt aaccacgcga gttggcgaag cgcagctgca
      361 cggggaggta ttccttgccc cattctgccg tcgcaaagcc tgccgccaca ttctgcgccg
      421 gatcagcccc cgcgggaacc tgcgcggcga actgcgcgac catttgcagg aacgcttgcc
      481 gcgccgacac ctgccacttt tcgttttgcc cgaggttcgc tttcttggcg ataagcgaaa
      541 tcagctccag gtgcgagcgt gcatcaccct gcggggccac cgctttgcgc agacgctgaa
      601 ccaggttttt ggcgtttgta aacgtgccgt cgatttccgc ccaggccgcc gctggaagct
      661 gaacgtgcgc gcgacgcaga atcggtgaat cctgcaccgc ttggcagatg aacagatcca
      721 gaccgtcgac gagcggtgcc acatcaaact gcgcttgttc ggaaagcggc agatccgcac
      781 cgaccatcca cagcgccttg agcttgcccg ccttgaggtc agaaagcagc gcgtttgcgt
      841 ccccagcccc ggtcccagcg agcagtttgg cgcccgcggt gttcgggttt ttgtcggcac
      901 tgcgcaggat cttgtcggca cgctcgggac tcgccgccac gcccgttacg taggtgcgaa
      961 cactggcgcc acgcgccgca agcttggccg cgatctgcga cagcgcaaac agcggttcat
     1021 tggtcgtctg cgctgaaaac acaagaccaa tcgtgccgcc ttggctggcc aaacggtcca
     1081 gtcgttctgc tgcaaacgca atcgctttgt cgacgctggt gatctgcgcc ggctgacccc
     1141 gaccggctcc gagccgcgcc gatgtcagac gcttgtgccg cacgtctttg tacgtgaacc
     1201 ggccttcgtc acacatccag tacttgttga cctggaggtt ttcgcgcgga accagacgcc
     1261 aaatttcgcc ctgcgcgtgg tggacttcga cgttacagcc cgttgcacaa ccagggcaca
     1321 ccgacgaagt cgtaaacaac tcccacgccc gcatcgtaaa gcggaagtct ttgctcgtca
     1381 gcgcaccgac cggacagaca tccaccgtgt tcagcgaata cggattgtcg agctgatgcc
     1441 ccggcgccac cgtcagttgt tcacggtcgc cacgccacgc catcgtcagc tgtttttcgc
     1501 ccgccacttc gtcgcagacg cggatgcagc gggtacacag gatgcagcgc tcggcgtcga
     1561 gcacaatgtg cggcccgatg tcctgcatct tcggcttgtg cactttgggt atcgacgagc
     1621 gcgagccgtg gtgatcccag tcctggtact gcttctgaag cgtgcactcg ccggccttgt
     1681 cgcagatcgg acagtcgacc gggtggttga ccagcgtaaa ctccagaatt tgccgacgga
     1741 tggtgagcac gcgcggcgtc gccgtgttca cttccatttt gtcggtcacc ggagtaaagc
     1801 aggccggcac aagctttggc tgaccaacaa cctcgacgag acactgccga cacaccgccg
     1861 gagagctgag ccccggatgg tagcagaagt agcttacgtc gacgccgtgt tgcagacaga
     1921 cttcaagcag gttggcaccc ttcttggctt ggacttcgcg accgtcaagc ttgatggtgc
     1981 aaagctcgct cggtggcgga gcgctgattg cggggccagc gggtgcagtg ctggctgggg
     2041 cgggcggcgc agcagcgggc ggtggcggcg gactggccgt ctcacgcagg gcggctgcga
     2101 tgctcgactt gatgggggat gaaggggtct ctgccatgac ctacgttgcc ttgttggtta
     2161 gcggtcgcac tcgccgccga tcatgttgat catgccgaac gttggaatga tgtcggcgat
     2221 gccggctccc tgaatcagct tcgacagcga ctgcatgatg atgaagcaag gggagcggac
     2281 gtgacagcga tagggccgcc ccgagccgtc ggagacgata taaaagccca gctcgccatt
     2341 gccaccctcg gtatacgagt acacctcgcc gggggggact ttgattccgt cgatgaccag
     2401 cttgaagtgg ccgatcatgc tttcgatggt gttgtagacg gcgtttttcg gcggcataca
     2461 gacgcgcggg tcgtcgagaa ggaacggccc cggcggaatc tgtttcatcg cctgatcgag
     2521 aatccgcatc gactgacgga tctcttcgag tcgcaccgca aaccgatcgt agttgtcgcc
     2581 gaccgatccg accggaacgt cgaactcgaa gcgatcgtag accaggtagg ggtgatcttt
     2641 gcgcacgtca tacggcacgc ccgtcgagcg caagcacggt ccggtccagc cataggaaat
     2701 cgcgtcctcg gcagagatca ccgcgacgcc gtccatgcgg tctcggaaaa tgcggttttt
     2761 gacgagcagg ctctcggttt cgcccagcgc ttcctggctc ttgtccagcg tgaagcgcag
     2821 cttgtcgagc cagtgctccg gcagatcctg cgacacaccg ccgatgcgaa cataggagtg
     2881 ggtgagtcgc gcgcccgtca gctcctcaag aagctcccac agccactcgc ggacttttat
     2941 catccacaaa aatggcgtaa agccaccgac ttccatcgcc gacgcgccaa gacacgtcag
     3001 gtggtcggtg atccgggaaa cctcgccgat gatgacgcgg atgtatttgg cccgctcggg
     3061 aagctggtcg acgatgccga tcagcttttc aaccgcgagc gcaaaaccca cgttgttcag
     3121 cagcggcgag acatagttca gacggtcggt atacgggaag acctgcgtat aggtcgccgc
     3181 ctcggcttct ttttcaaagc agcggtgcag atagccgact tggacgtccg aatctttgac
     3241 cgtctcacca tcgatggtca gaatgatccg cacggtgccg tgggtcgccg ggtgcgacgg
     3301 acccatgttg atcgtcatcg gctccgtcgg cagctcgatg tcttcgacct cttcaagctc
     3361 ttcggtgttg agcttgtcca tcaggtcttg cggcaccttc ggacgcgcgc taagcccagg
     3421 gattcccccc gccgcgatct cgtcgggagt cggcattcca aacgcgagct tctttggccc
     3481 ggtcttgctc tgatttgtcg tcgtcgacat gtctttctgt tcccttagct cagcgagtag
     3541 tcgcggcgaa tcagcggctg ccgtttttct ttgggatagt ccttgcgcag cggatggccg
     3601 acgaactctt cgtacatcag aatccgacgc agatccgggt ggccggtgaa cttgaccccg
     3661 aacatgtcgt agacctcgcg ctcctgccag ttggcggtct gccacagatc gctcaccgtc
     3721 ggcacttctg cactaggtcc gccttcttct tcgtgcaggc ccaccttgat gcgcagccgg
     3781 tttcccgtcg ccatcgagcg caggtgatag accacttcga agcgcggctc gcgcccaaaa
     3841 taatcgaccg ccgtcacatt cacgaggaag tcaaaccccg ccgtgtcgcg cacaaagcgc
     3901 atgacatctt tgaagctgtc tttgcttacc accgccgtat cgtcgccgtg gctgctgtgc
     3961 gacgagacaa tcgctgcggg aaactgcgcc accagcttgt cgagatcagc tttgctcatt
     4021 tgcgaaatct tcccttgctg cctacttctc acgaggggcg gggtttaggc gaccaccggc
     4081 agacgcttgc gcccgtcggt ggaagccgct tgcagctcga cttctttgcg gtcgcgacgc
     4141 tgcacccagg ttgtcgcttc ctgaatcttc ttttgcagaa gaatcagtcc gtcgagaacc
     4201 tgctcgggac gcggcggaca gccgggaatg tagacatcca ccgggacgat ctgatcgcag
     4261 cccggcacgg tgttgtagtt ctggtaaaag ccccccgtcg aggcgcagac gccaaatgcg
     4321 atgacgtact tgggttcggc catctgcatc cagatgcggt gcagtaccgg ggcctgccgc
     4381 tcggtgatcg taccgaccac aaacagcagg tcggcttggc gcggcgaaaa gcgggggaac
     4441 tcggcaccga agcgggcgat gtcgtacttc gacgcggcgg tcgccatgta ctccatgcca
     4501 cagcaggcgg tgacgaaggg atagctgaac agcgaatact tgcgggccca gttcagggcg
     4561 tcgtcaaggc gggtcgtgta gctttcggcc atggttcttt cctgactccc tgcttagccc
     4621 cagccgatgg cgcgcttgcg ccaaacgtag gcgagcgcga ccaccagcgt gctcataaac
     4681 accagcatct cgacgaaacc gaatgacgtc acggtgccaa cgcactcgct gtgaatcacc
     4741 gtacccaggc aggacagtcg acggtaaatc gccgcccagg gatacaaaaa cgccgcttcg
     4801 atgtcgaaga ttagaaacag gagcgccacc tgatagaact tgacggaaaa gcgcacgcgc
     4861 gggctgccaa tgggttccga gccacactcg aagggcgcga gcttttcggc gttttcgcgt
     4921 ttcggaccca ggaacatcgc cagagccaag aacacgcacg aaaacgcgat gccaaacagg
     4981 atcgccacga gcgtggggcc atagtcagtt gcttgttcga cggacattcg gaacctcgtt
     5041 tgctccgccg cgaatataaa tgggtggcct tctgggtgtc aatcttcgcc cacggagctt
     5101 gttcgattct tccgtgagat gcctcagtcg ggtgttcctg cttctcgggt cggaccacct
     5161 acattgtctt tggcagcggc tttggcggac tcttgccggg cttttgctga aagcaggtgt
     5221 ccgagcgccg tcttggcttc atcgcgaatc tgtgcgtcgc tgtggctctg cgcgacaaac
     5281 tccaaatagc gctgtgcttc gtccccaccg agcgccgccg ccgcttccag cactttgcca
     5341 agctcactgc ggtcgcgaaa ctcggtgcta tcgagcaggg gtcgcaccga cttggggtcg
     5401 cccagctcca ccagtgcacc aatcgcctga tcgcgcagcg cgcttggcga ctgccggtct
     5461 tttagcaccg ccatcaccag cttctgcgca ccttgatctt tgcgcatcgc caaaagccgc
     5521 agcgccaccc cgcgcaagtc cggctctttg ctgttgtcgt tggccagctt ggtaagctcg
     5581 gacgttttga ccccacaaag tcgcagctgc gcccccaagc tgtgagacgt atcccgcacc
     5641 gcccgtcgcg ccaagtccga ccacacaagc cccgttggtg atgtcgtttt ccctgactgt
     5701 tccgccacgg ccaagtgttg cagccgggta agctctggtt gcggggtttt ttcgtcgctc
     5761 atggcatcgc caccgcgccg cgtccgcaaa agccccgcgg tgagcgcgcg caaaagcggc
     5821 ggcttgggct ttgactcggt tcccgccgaa tagaccaagc ccagctcgat ctgcagcacg
     5881 aagtcccctg gctcacgttc cgctggggcc agcgccaccg cgacatcggc gctgcgcagt
     5941 ccttcgatcg ccgcagcgag cagcatgtcg cgatccagtt cggtcggtgc tttttgctgg
     6001 gccgcactgc cgtcggtctg gctggcgatc tggatgtcga ccacccgcat gggcagcgtc
     6061 gcaggcgtcg gctcagggcg cttacaaccc acgcacacgc cgaggcacaa cagcattccc
     6121 agccagacag cccatgtgcg acgcttcatg gcggcaagta tagcgtggag ccatggccac
     6181 cctacgaccc cgcagatgtg accaaactgt cacagcgacg acaccgattt ggcacggcgc
     6241 ggccaaattg gttggatctc cgacgacagc attgcaaaac gggcgcgaaa taattatctg
     6301 gccatgacaa tcccatgaca tgcggtgggc aacatgacgg aatgcagaaa aaaccccagc
     6361 gtcccgtgcc ttccgtcgaa atcatcgaca cgccgcgcgt ccgtccgccc cgtccgatcc
     6421 agatcgacaa gctgacgagc attccgtttt tcttggtcca cattgctgcg ctggcttcgg
     6481 ccatttactt gggcgcaacc aagaccgaca tcatcgtctg cgtcgctctt tattacattc
     6541 ggatgtttgg aatcaccgcc gggtatcacc gctacttttc gcaccgcgca tacaaaacgt
     6601 cgcgcttttt ccagtttgtg ctggcggtga ttggtggtgc gtcgatgcaa aaaggcgtgc
     6661 tgtggtgggc ggcgcaccat cgtcatcacc acaagtggag cgacatgtcg gaagacattc
     6721 actcggtcaa gcaggacggt ttctactggt cgcacgtcgg ctggatcatt tccaacgagt
     6781 acgacgagac cgagtgggat cgcatcaagg acttttcaaa ataccccgag ctgcgctggc
     6841 tcaatcgctg gcactgggcg ccggccctta tcggtggcgc gatcctgtac gcgatcggcg
     6901 gcattcactg gtttgtgtgg ggcggtctgg taagcagtgt gtttttgtgg cacggcacgt
     6961 tcttcatcaa ctcgctcgcg cacgtttggg gcagtcgccg ctatccgacg accgacacca
     7021 gccgcaacaa cttctggctc gcgctcatca cgatgggcga aggctggcac aacaaccacc
     7081 actactacca gagcaccgcc aaccagggct tcttctggtg ggaagtcgac tttagctact
     7141 acatcctcaa gatgctgtcg tgggtcgggg tggtgtggga tctgcgcacg ccgccacgcc
     7201 acattttgga aggcaaagcc gcggtggacc cgatcgagcc cgaagttgcg accgaagtcc
     7261 ccgccctgcc acccgctgcg gttccatccg tctgacctgc ccgtgataca ctggccgcca
     7321 cgattttccc gcgaggggga gaggccagat gtcccaaaac aacgccaccg attggcaacc
     7381 cgcacagccc ggcgaagggc gcagtccatg tcccgcactc aactcgctgg ccaacggcgg
     7441 gcatcttcca cgaaatggca tcgccagccg ctcacagctc atcactgcga tcgtgggtca
     7501 gcttggtatc tcgacggagt ttgccaacac gctggcgaac gcggcgcttg aaaaattcgg
     7561 caaacccggc gacggtggcg aacaagttct gcatcttcag gatctgtgtc agcacggcaa
     7621 gctggaacac gacgccagcc tgacccgcca agacgcccac gccggcgatc atgccaaggt
     7681 tgatccatcg ctcgtcgaac agctgctgtc cctgagcaaa aatggacaga ccctgacgct
     7741 cgatgatctg gccgcggcgc atcaaattcg catgcatcag tcggcgcaag gcggtcacca
     7801 tgtgccgggc aaagccggtt tcgtggggac ggtcgaagcg gcgctgctct acaccgtgct
     7861 cgcccgcggg gaatcgggca tcagcttggc cgatgcgcgt gaatttttgc tgaccgagcg
     7921 cgtgccgcaa ggcctggccg gacgaaacat cacgctggcg caagtcgccg tccgcgccat
     7981 cgccatcgcc gccaaaggca acctgcccgt ctgtgaagcc gcccgtcgcg ccaaagaagc
     8041 gaccaagaag taatcccacc aagaaaaccc cggcgtgcgc cgaaaagccg ggctaccatg
     8101 cgccgcgcat gccgtttctt gaactgctgc tgacggcgct cgatacgttg cgcgccaaca
     8161 aagcccgcgc cgtgctaacg acgctgggcg tgatcattgg cgtgctgtcg gtgattttgt
     8221 tggtttctct cggtgagggc gcacgaagtt acctgggcga cacctttgcc ggactgggat
     8281 cgaacctgct gcaagttcag ccgggccgac gagaaaccaa gggctttggc ggtccctcgc
     8341 ttggcaccgt atacaaaatc acgcgcgaag acgaacacat gctggcccgt cgcgccacca
     8401 ccgccgatgg tgttgcgggc gtcgtgaccg gcggcgggac cgtgcgttat ttgaaccggc
     8461 gacgggacac gctggttctc ggtgtcggca gtcgtttcat cgaaattcgc cagatgaacg
     8521 tcgcccaagg ccgctttttt accgacgaag acatcgaatc ccgacggcgt ttcgtggtgc
     8581 ttggccgcac cgttctgcaa gagctgtttg gcgacgaaaa tgcgcttggc aaagtgatca
     8641 aagtcgccga cggagagttc cgcgtcatcg gcatcatgga acacaaaggc cagacgctcg
     8701 gcttcgacct cgacgacatt gtttttatcc ccgagaccac cggcctggac ctgttcgcgc
     8761 tcgatgggct ttcgcacatc ttggtccgcg cccgcgacaa aatcagcgtc gatcccgcca
     8821 tcgacgaaat caccgatctg ttgcgccgtc gccacaacga tcaagtcgac ttcacgatca
     8881 tctcgcagga cgacatgcta tcgacggtga acgggatcat gaacaccttt tcgatggtcc
     8941 ttgtcgcgat cgccagcatt tcacttgtcg tcggtggcat cggaatcgcc aacatcatgc
     9001 tggtgtcggt tcgcgagcgc acccgcgaaa tcggtgtccg tcgcgccgtc ggggccaaaa
     9061 aacgccatgt gctgatgcag tttctgctcg aatcggtggt gatttcgctg ctcggcggtc
     9121 tgatcgggct cggtctcggc gcgctgatca tcatggtggg gcgtcttttg ctgccgggac
     9181 tcccggtgcg gctgtctttt gacatcgtgc tgatcgccgt cggtttttcg gggatggtgg
     9241 gtgtgttgtc aggcgtcgtg ccggcgcagc gagcggcaag cctcgaccca gtggaagcgc
     9301 tgcgctacga atgatcatca gatgagcagc gaaaacacat ctgcgaagtc cgacgccgca
     9361 ccaaccgaaa aaccgcgctg gctgagcaaa gtggtgttcg gttttggcat cgcgagcctg
     9421 ctttccgatg ccggtcacga agccggaacg gcggctttgc ccgtgctgct cacgacgatg
     9481 gcggcggccc ccgcaacact cggcatcatc gaaggcattg ccgatggtgt ggtctgtttt
     9541 gccaagctgt ttggcggatc gctcgcaaac cgtccgcagt ggcgcaaacc gctggcagtc
     9601 accggatatt tggtcacggg gctgtcgatt ggactgttcg cgctgtcgac aaactggctg
     9661 cacatcttgg gctcgcgcat cctcggctgg ctgtttcgtg ggctgcgcag tccgtcgcgg
     9721 tcggcgatgc tggccgacgc ggtgccgaaa acagcactgg gacgcgccat cggcttccac
     9781 cgggccatgg acaccgtagg agcgatcttc ggaccgctct tggcgacgct gctgcttctg
     9841 cggttgccac tgcgccaggt gttttgggtc gcgatggtgc cgggagtgct cgccgccgtc
     9901 gcgtttctgc ttttggtgcc gggtcagacc acgacgtcac gcgaaccacc gccgtcgctt
     9961 ttgcgtggaa tgaaagagct tccacccgct ttccgtcggt tcctcatcgc ggtgatgtcg
    10021 tttggaatcg gcgattttgc ccgcaccttg ttggtgctgc gcgcgacgca gctgctgccg
    10081 gcccaggaca ttcccggagg agccgtgtcg gtggcgattc tgctttttgc gctgcatcac
    10141 gcagtcgccg ctgcttgtgc atttcccgtc ggatatttgg cagacaagat gtcgccgcgc
    10201 tggcttttgg ctgtgggtta tggtctgggc gtcgtcacag cgctgctcgc cgcgttcgca
    10261 cagccgagcg tggtctattt ggctgggttg tttgccgtcg ctgggatggt gattggaatc
    10321 gaagaaacca tcgaaggcgt actgtgcgcg aggctggctc tacccaagct gcgcggcacc
    10381 gcatacggaa tgctcgcagc gacgaacggc atcggtgaca tggtggcaag ttccgtcgtc
    10441 ggtgtcgtct gggcggctta cagtccacag cttgcttttt tgctggccgc agtcgcttgt
    10501 gccgtgggaa cggtgctctt ggtcgtcgcg gttcccgacg aataaatccg acgctatttg
    10561 ggccgattgg cagaaatgcg ctgccacgcg agcagacagc cgacgcagac cccaagcagc
    10621 ccaagtcccg ccgcaaacgt cacgccctgc cccactttcc cgtcgagcca cttgccgatg
    10681 taaatgccaa aaagcccagg tatcaccacc agccaaccga gcccgaccat cgtcacagcg
    10741 gtacttatca gaccgtgctg ttcgcgatcg tgtttttcgc gccgtgcgtg cagtcgctgc
    10801 gccgaatcgg gcagcgcatc gggcttcgag cctggtttct gggttgcgcc ttggctcatt
    10861 gctcgctcaa cagccgccgc ggcccgtttg tccgatgttc gtgtcgcaga tagtcggtca
    10921 tgtgactgag caaggcacct tccatccgcg caaagctccg gtacgcctcg ctctcttgcg
    10981 ccaccgcgag agacagccga cttttcactt ccgccgacag cgcccgcaag tcatcgcttg
    11041 gcgtcgcttc gcgggtgaag acttgcacat ggcgcccgcc gctcacgaac aacaccccgc
    11101 cgcgcagacc gacgaaatgt tccaccgcag gatcgtcggg tccgccgcct gcgcgataga
    11161 tcagcaccga caccgacagc accgtcagca agtcggcatg accgggcaat attccaaatg
    11221 atcccgtcgg atcttcggct cgcacatgcc gaacgccgtc gagatcgagc acaagctgaa
    11281 gcggcgtcac aatcgtgaga cgcagacttc gctgcgacgg ctcaagctct agcgaggcat
    11341 cggccatgcg cgtcctgggt tagtacgaaa gaagtgtcac cgccggatgt ccgccaagcg
    11401 ccttgcgccc atgcaaaaac gacagctcga ggacaaagcc gtagccgacc acctcggcac
    11461 cttgtttttg acacagccgc gctgaacctg ccgctgttcc gcccgtcgcc agcacgtcgt
    11521 cgatgatcac aacccgctga cccgcaacgg catcttcgtg catttcgagc gtcccgctgc
    11581 cgtattccaa gtcatacgtc tcggtgacgc gacgataggg cagcttgccc ggcttgcgaa
    11641 ccggcgcaaa acccagaccc aaacgctccg ccaccggcgc cgcaaaaatg aagccgcgcg
    11701 attccacccc aacaatcatc gtcggtttga gcggcgcgag tcgttccgcc aaaagatgaa
    11761 gcacgaggcg aaaagctgtc gcatcagcga gcagcggcgt gatgtctttg aagagaatgc
    11821 ctggcttggg gaaatcagga acgtcgcgga tgtgacgagc gagcagcgcc agggcaggat
    11881 cggaaaccat ctgtgcaccc tatcatcgcg gcagggtttt ccaaagcccg cgctacatgc
    11941 ccacgcgacg cattccgcca cccggaggac caccgccccc gcctgaccgt ggaccgccca
    12001 tgggatttgc gccagaaccg ccgcctgccg acttcgccga atcgacgatg atcgcttggc
    12061 cttcggttaa gccgtcgctg acttcggtaa acagaccgtc gctgatgcca gcgctgacga
    12121 agaccggatg gaagcgctcc ccgtcgggaa caaagatcgg cgacttgttg cgaccttctc
    12181 cgcggccttc gcgctttcgc tcgcggcctt cgcctttgtt ttcgcccggc gcccgatcga
    12241 gcctcggctt aaagcgcagc gccgcattgg gcacaagcag cacatctttc cgttcggcga
    12301 cgaccacccg cacgttggcg gtcattccgg gccgcagcaa aagttccccg ttgtcgacat
    12361 ccagcaccac gacgtaggtg acgacgtttt gcaccgtctg cgctgcgttg cgaatctgcg
    12421 acaccttggc ggcaaagcgc ttgccccgga agctatcgac ggtaaactcc gccaccgccc
    12481 cgacgcgcag atagccgatg tccgcttcgt cgacgttggc cagcacctgc atttttttca
    12541 gatcccccgc gatctggaac aggttgggcg cttggaacga tgcgacgacg gcttgcccca
    12601 gttcgacgtt gcgcgaaatc acgatgccgt cgacgggaga caagatcacc gaatttttca
    12661 gatccagccg cgccttgtcg agcgctgctt gcgccagctt cacacccgct tccgccgcct
    12721 ggacgctggc tgcggtgctc tgttccgtcg cgacggactg atcgcgatcc gccgccgaga
    12781 tgtaactttt ggcaaacagc gcccgcgatc gctcggcaat ccgcgatgca ttgagcgcat
    12841 cagccttggc gcgaatcaag ttggcctgcg ccgacgccac gttcgcttct tggcttttga
    12901 ctgccgtttg aaacagcgcc gggtccagct cgaccagcac ttgcccttgt ttcaccgtgt
    12961 cgttgaagtc ggcgtgcagc ttgtgaacga tgcccgacac ctgcgaaccg accggggaca
    13021 aaagcaaagg ctgaatcgtg ccggtcgcat tgaccacctg cgtcacgtca ccgcgcgtca
    13081 ctttcgcagt cacccagcgc ggcccttcgc ttttgtgaaa aaacagccga taggcgccaa
    13141 aaccgattgc caccactgcg accaccgcga gcagccagat ccagatcttc cgcttcgggc
    13201 tggtcttttc cgtcatcgct tcgctcatgg cgtcgcatct cctcgtggct cggtttcttt
    13261 gatgagctct cccaggtaaa agcgcaagct cgcccgctgc tgcgcgagcg caaagtcagc
    13321 cgacacccgc tgtgcctgcg cctgcatcgc cgcgacttgg gcatcgccca gctcaatcgc
    13381 gttgccgacg cctgcccgat agcgcccatc cgccatctcg agctgctttt tggcctggtt
    13441 cgcgaccgcg accgcggctt cgaccgtcag ccacgccgtc agcacctgct gaagcgcctg
    13501 ctgaagctgg ctgcgcacct gaagccgcag ctgcgtcagg ttcgcttcag ccagcgcaag
    13561 ctgagccctc gcgtcacgaa ccgcatacac cgtcgacaaa ccagtgaaca ccggccaggt
    13621 cagaccgacc gttcccgata ccaaaagtcc tggcagcgct tgcgtcgtga cgtccagacc
    13681 gctcactccg ccaaatgtcc ccgagttcat ggtcgcactg gcaccaagcg acagcgacgg
    13741 caaataatcg gacttggccg ccttcagctg cgcttcgctt tgccgcaccc gcgccaacag
    13801 cgactgatag tccgggcggg cttgcagcgt ccgttcaacc acttcgtcgg gcaccgtcgc
    13861 aaccgtttcc gataccgcgt tgctgagcgc ggcttcgatt ggatgcggtg ccggcagcgt
    13921 cgactgcagc ggtcgcttgt gccagcttcc ccagtctcgt tccggcagac ccagcgcctg
    13981 caaaaactgc gtccgcgtca cttgcacatt gccctgggcc tgcagcactt gcagcttggc
    14041 ctgcgccacc gccgtttggg caatcagcac atcgatctgc ggcttggtgc cgattttgaa
    14101 aaagctctcg gactgcttga gccgccgggt ctgttgctgg agattttcgc tcgccaccga
    14161 cagcagcgcc tcggcttgca gcaccgcgta gaagctgctc atcgtcgaaa ggacgacctg
    14221 cgcgcgcgcg tttttttcat cggtcaagga cgcttccgca ctggcctgcg ccgccgctat
    14281 gcgccccgac agccggccaa agtcccagac cagctggttt agcgacagca caccgcttga
    14341 acgaaacgtc gagaccgagc tttttccctc gggcgcgcca atttgatagc tgtaatcacc
    14401 gcgcaccgag cccgacagcg aaagctgggg cagccacgga ctgcgcgccg ttccgacttt
    14461 tgcttcggat gaaagccggg tctgccgagc cacttccgcc gacggattgt ggaccagcgc
    14521 ggtctggatc gcctcgacca gcggcagagg cggccccgac agactgctga ccgttggcgc
    14581 ggtgtctgca cttggctccg tcgccatcac cggagccgtc agcagcatcg aaagcgcgcc
    14641 cgtgcaaagc caagcccgcg tgttcatcag ctaccctcca cggatcgcca agccaactgt
    14701 cgcttgtgct ggaactgccg ttccaagaag cgatcggacg ccccaacatc tgcggccagc
    14761 gtcgacaaag tggtcgcgaa aaccagtccg tagaccgact gacgcgacac cttgaccggc
    14821 tgatcgggcg taaccagcgg cgaacccaca ttgcccagca tcgccagcgt atcgagcgcc
    14881 aaaaaaagcg gccacacaca ggccagacgc agacgaacca gcgacttggg aatcgccagc
    14941 acatatggcc acgcttcatc gcagcggcgt atgccaagcc gcagtagcgt cgccgtcagc
    15001 ttgcgcatcg cccgcgcttc gcccagatcg cgtggcggcg tgcgggtcag atcataaagt
    15061 cgcgcgcagg tcaggccatg ttcgtcgagc agcggctgcg gaatgtagca gcgaccgatc
    15121 cgcaagtccg ccgccagatc gcgcaccaca ttggtaagct gaagcgcttt gccaagcgac
    15181 acgccacagt cgacaagctc cggcgagcgc aggtgcgcaa gtcctggcag gtgtgcagca
    15241 cacagctcgg tccaaaactc cccgacgcag cccgccgcgt agtaggtgta ttcatcaagc
    15301 tccgccagcg actgtagcac caccacctgg tccgccgacg acacactgcc cgccgccgga
    15361 aagcgacgca gatccgtctg catgccgctc accagctggc ccagcacccg cgtcaccaga
    15421 cggcgatcag gctcatcggc ttgcagatgc agcgacagac actcgtcaag caccgccaag
    15481 agcgtctgct cgctcgacgg caacgcctga cccatcggct ggcgcaaaag ttcccgcagc
    15541 gccagctgaa gacgggcgag tgccgcgaca tcaccttcgc tggcagaaga cagctcccca
    15601 agcagcgaaa gccgggtcgt cgcaggcaaa agatcggtgt ccgcgatggt gtcagccgcc
    15661 cgagcgagca aatagccaag cccaatcggc gcagcaacct cgtcgggaag cacacgaacg
    15721 ctaaggaaaa atgaacgaga gacgcgcttg agtagcgcgg tgagcggcgg cgggagcacg
    15781 ggaggaagtc ttacttggca ggcggggtcg gcggcttgac agcaccgggc gctggcgtcg
    15841 gcggagtcgg cggtcgctgc cccatcgctt tgagcgcagc cgcttccgcg agagccgctt
    15901 gccgttcgtt ttcttccgcc ggaccgatgt gctctttgtt cgtgtcatcg agcatcttgt
    15961 gatagacgag cagtccgtcg ctcgacgtga agcccttggg gctcgactga ttgaacacac
    16021 cttcgaagac gtactgtttg ccgatggtca gcttcggctc gcgaccgcgg aaaccacggt
    16081 attcggcgat cagcaggccc ttttctttgc tgccgttggc ttcgtcggat gcatacatgt
    16141 gcggttcttc gcactgcttg cactgctggc ctttcgggca gaccgggcac ttgtacacct
    16201 cgaggaggta ggctttcacc ttgaccttct gcccgaggta cttgttcttg gtgtcgctat
    16261 agcgcttaaa ctctttgcgc agaccgtaaa tggacaccgt tccgtcggga tactcacgcg
    16321 ggatgttcgg ttcggcaaac gacggcggcg gcggcaggtt cagcttcaca ccgggcagta
    16381 gcgtcgcacc gactccggaa ttgtcgagaa gcccagcgag tcctttgtca cagcccggca
    16441 caagcgaagc aagcaacatg aggagaggca gccagccggc tcgacggcca aaccgacgct
    16501 gctgagctgc gaagaggcgc ccgaggcgga cgcgaccgaa agcggatgcc aagttcatct
    16561 tctgccattc ctcctctgcc gcgcggcaga gcgccaaaaa ccacgaccgt cttgcagctt
    16621 tgcaaaatcc ggtacgtaag taggtttact tatatcgctc gcgatagcaa ttggggtgct
    16681 gattcgttag cacgacggcg gggcccgaat caacttccgc ggctgcggga acatccaacc
    16741 acctgggcaa aagccgcctc gccacctgcg cccagtccgc ttgtgctgct tgcttgggtc
    16801 cgtgcgcgcg cagccgggcc gccaacatcg gatcgccgag cagcgtctcg atcgcttgcg
    16861 caagcgcttg tgcatcaccg ggtggcaccg caagaccaca gccgccaagc tgcgctgtcg
    16921 tcccacccac cgccgatgcc acgaccggca gtcccgatgc gagcgcttcc caaatcacca
    16981 ccggcgcgcc ttcggtcctg ccatcgggca attgcaaaga cggcagcacc agcagatccg
    17041 cggcggcaag ccactgtcgc ttgtcacgac cgagcacctc gccaacaaaa cgaaccggca
    17101 gaccctgcgc gtgtgcttcc gcttcgagtg gactgcgcaa aggaccatcg cccgccacga
    17161 tcagctcgat gtccaggtgg cgaagtctgc gaagcgccga gagcaacacc gaaacacctt
    17221 tgaccggaac cagccgaccc aaacacagca gcgttggacg atacaggccc agtttgtgcc
    17281 ggatcgcctg ccgttctccg tctcgcaagg caaattcggc aacatcgatg cccatcggca
    17341 ccacgcgccc tggtgccccg tcgatgacaa gatgttccgc ggtgtagacc agatcggcac
    17401 gcgccgcgaa ccagcgtccc atgttcgcaa gcccaagccg ccgcaacagg tgaacgtccg
    17461 acgaatgcgc aatcgccaca tgcgggcgcc ctcgactgac aagacccgcc acaagaccgc
    17521 acggcacaag ccaatgcgaa atcagcgcgt cacagacgga caaaaactgc gccaccgcgc
    17581 gcacaagatc gacggaaaac tgcatcgcca ggcccagggt gccgatcggt ttgcgccgaa
    17641 gaccctgcac cagtgcgtcg ggagcgccgc cacggaaaaa cagcgacgat ggcagccgat
    17701 gcacggcgat gccatccagc cgctcaaaca ccgcggcgtc ggaatcaccg gcacacacga
    17761 cgctgacgcg atgcccaagc cggtgcaggt ggcgattgag tccgtgcaca aagccgcccg
    17821 ctgggtcccc ggcaaagcgc ggatacgacg tgctgactat tccgaagtgc atgggtccac
    17881 cacgcgcgtt tgccttcgac gccagcgtgc ccacagccgc cacgggagcg cccgcagcgc
    17941 cacaaacaac accggataga tcgccgtccg cagcgaaatt cccgaacgcc aggtccgccc
    18001 gtagatggtc cgcaccggca catcttgcac ccgcacgcgg cccacccaca gcctcgcgag
    18061 cagatcgttg ggatagccgt agcgcggaaa cagcccgtcg aggtcaatcg acaaaagcgc
    18121 tcgccgcgtc gctgccgtaa agccacactg cgaatcgaac atccgcggat aaccggcggt
    18181 gattcgggtc aaaagcgaca gcacaacatt gccgacaaga cgcagccgcg gcatcgtctg
    18241 ccagacttcg cggtgggcaa atcggttgcc ttttacataa tcggctcgcc cgtcgacaat
    18301 cggctcgata agcgaaggca catcggcggg gtccatctgc ccatcgccag ccatcacgac
    18361 agcgacgtca ctgtttagca aaagcgcccg ccgataaccc gttgccaccg ccgctccgac
    18421 gccgcggttt tgcaggtggc gcagcacttc gacgccgggc cgcgcatgac aagcggccac
    18481 cgacgacgtt tcgtccacgc tgccatcatc gacgaccagc acatgatcaa cataggtcgg
    18541 gatcgtcgcc agcgtgctcg gcagatgatc cgcttcgtta taggccggga cgaccactgc
    18601 gacccggagc cccttgtaca tgacttcatc ccatgccgcc ttgctcggcg acggcttgcc
    18661 gcaaaaagcg cgcgtttcgc gctacttgtc accgacgaca attttcccgt cgatgatgtc
    18721 ttttcggaga gcttcgacgc gcgcgcgaac gtgggtcggg atcatgccgc gacgaggttc
    18781 gtcgtaggcg tagtcgatgc cgccttcgcg caggccgaac actttgacac ccggatcgaa
    18841 tcgaccttcg cgtagctcgg cgatgacgtt gtagacgctg acttcgatcc gtttgaccat
    18901 cgacgtcagc atgtaaccgg gcgcgtcgtc ccactgatcg ctgtcgacgc cgatggcgcg
    18961 ccgatttttt tcacgcgccg cttcaaatac gccaagtccc gtcgaacccg cggcatgaaa
    19021 caccacgtcg gcccccgcct gatattgggc cagcgcaagc tccttgccac gcgccggatt
    19081 tttgaacgca tcggcacttg taccggcata accgacgagc actttgcaat ccggacagac
    19141 cgcgagcgcg ccttggcgat aaccggactc gaacttgtga atcagcggaa tgtccatccc
    19201 gccgacgaaa ccaagcgtct tgtgcggtga caaaagcgcg ccgactgcac caaccaggaa
    19261 gctgccttct tcttcgcgaa acttgagcgc gaccatgttg cgcggcggca ccacaaagcc
    19321 atgctcgtcg aacttcgcgt agtcgatgca ggcaaagtga cgatccggat agtcgtgcgc
    19381 aatttggagc atgtcgtcgg aaaaaacgaa accgaccccc agcaccaaat caaacccgcg
    19441 cgccgccaga agccgcagac ccgattcccg atcggcacca tcgcctggct cgacaaactc
    19501 agccaaaatg cccagctcgc gcacagcctg atcgacgcca cgaaacgccg catcgttgaa
    19561 cgacttgtcg ccccgcccgc cgacgtcgaa caccagcccg acgcgcaaag gtcgccggcc
    19621 agaatctttg actgccgtcg aatcagcgcg cagcggccac agcaaaagtg cgtttatccc
    19681 caaaaggacc aacgtcacaa cgagcagcaa gcttcgcgat gcagctttca cgccggaagc
    19741 tataccacat gtggtaaggc agccagagag ggtcgcaact gcctatgctg atgaacggaa
    19801 gcgaaaacca gttctatttt ggcctgctga ttgcctattg ggtgctcggc ggactgacgg
    19861 cgatcaacct gatgtttcga tccgcgccgt atggtcgcta tgcccgcaaa ggctgggggc
    19921 cggacattgg cgccaccgcc ggctggatca tcatggaagc gccggcctcg cttgcgccgc
    19981 tcgcactgtt tttgctgagc gaccgccgag acaccgtgct gtgggtgttc accctgattt
    20041 ggcagctaca ctacttccat cgggcgtttg tcttcccatt ccggcgacgt ggcggcggta
    20101 cgatgccact ggcgattgcg ctgaccgcgg tggtcttcaa cctggggaac gcctatctca
    20161 actggcgatt ccttgcgcag cttgggcccg tttacgatcc aaactggctc actgacccgc
    20221 gctttgtcgt cggcacagcc gtttttttgg tcggctttgt catcaaccag cacgcagact
    20281 atgtactgct tcacctgcgc aaacccggcg agaccggcta caagatcccc tacggcggca
    20341 tgtaccgctt tgtctcgtgc cccaactact tgggcgagct ggtcgagtgg atcggctggg
    20401 cgattctcac ctggtcactc ggcgggctga cgtttgcatt ctggaccgcg gcgaaccttg
    20461 tgccccgtgc gctcagtcac catcgcgact atcacaagcg cttccccgag tatcccgccg
    20521 atcgcaaagc cgtgattccg ttcctgctgt agcggacggg caaagcagca gcgatcaccc
    20581 aagccgcgac gtgggcggct tggcggtgac acagacggat tgattcaggg aagaggacgg
    20641 agcgggaagg ggaagcgtgt ttcgcggaag ctggaaccgg acaggccgtc gaccggggag
    20701 gccgacggtg gggactattc ctcgtcctct tcgtcctcgt cctgcttgcg acgctgcttc
    20761 ttgcccttct tcttcagcac gtcgccgagc ttggcaccca gttcacccga gccaccgtag
    20821 ccctgtgccg ccgagacagc cgctgcgcga ttggccgcct tgatcgacag accgatcttg
    20881 cgctcttgcg ggtcgagcga gatgacctcg acgttgactt cctgaccggg cttgagcacc
    20941 gtgcgcgggt cttcgacgcg gtcgtcggag atttccgaga cgtgcaccag accctcgacg
    21001 cccttgtcga gctcaacgaa cgcaccgaag tcgagcacct tctggacctt ggcgggaaca
    21061 atcgatccga ccttgtactt gtcgggaatg atgttccacg ggtccggatt gagctgcttg
    21121 atgccgagcg aaatcttcgg cttttcaccg tcggtggtgt cgatgttgat gaccaccgct
    21181 tcgacttcgt cacccttttg gaacatctcc gacgggtgct tgatgcgctg ggtccagctc
    21241 atatccgaga tgtgg
//
LOCUS       DAORGN010000074        20319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1039_length_20319_cov_5.990426, whole genome shotgun sequence.
ACCESSION   DAORGN010000074 DAORGN010000000
VERSION     DAORGN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_20319_cov_5.990426"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1151)
                     /gene="argC"
                     /locus_tag="PKL17_10150"
     CDS             complement(108..1151)
                     /gene="argC"
                     /locus_tag="PKL17_10150"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNK45135.1"
                     /translation="MNPALKVLVLGAGGYVGGELLRLLLQHPHVAKVTAHSRSQEHKA
                     IADVHPTLLPLSDARFVGGELVAQFADVDVVMSALEHGELSRLLAPLWEKKPPLLIDL
                     AADFRVADPALYARHYGPHPSPDLLSQFVYGLCDVEGPKLRGATAIAAPGCFATAAQL
                     ALYPFRDLIGASIPALFAVTGSSGAGAKPRDTTHHPARAHNLFAYSSFSHRHEAEILQ
                     SLRRWTGRAESTARLITHSGPMVRGIYLTAHLVVEKPLAAEAAAQALATAYAHRPLVR
                     VLKKPPDLTHALGGSYALCHACQSDDGREVQVTVAIDNLLKGAAGQAVQAMNLALGLP
                     ETAGLQMVGAFPC"
     gene            complement(1148..2239)
                     /locus_tag="PKL17_10155"
     CDS             complement(1148..2239)
                     /locus_tag="PKL17_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45136.1"
                     /translation="MLTTRVRLDRIASSTKNALLGRDVIVSDQITAAEGFVLAVRILE
                     DKSTYNTVEDLTGRMVPLRAGDILAGTLGNRRALHGYAGEVPSHISVGDTVHVLNLGG
                     VLGRCTSASPELGPPFRAEVLGAILSFPELSDRVGRPANIRQGAVPTADDLRSRVPVI
                     YVAGTCMNTGKTYAATELVRGLTRQGVRVAACKLTGVSLMRDTLQMLDAGAITALAFS
                     DSGLASTRAGVTTATAKGIFNRLSDAKPDVIIAELGDGILGEYGVQDILGDQALRGAA
                     GAWVVAAPDPVGCWGAVEILRRDYGIAATVLTGPATDNEVGLDYIRSHLSVPAHNARR
                     DAAGLVAVVRRAIDSAPKKAAAACAEDKP"
     gene            complement(2239..3471)
                     /gene="argG"
                     /locus_tag="PKL17_10160"
     CDS             complement(2239..3471)
                     /gene="argG"
                     /locus_tag="PKL17_10160"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNK45137.1"
                     /translation="MQTKRGPLALAFSGGLDTSYCVPKLSEAGWTVHTVFVDTGGTSS
                     DQREAIAKQAHAVGAASHHAVDAREQVFDRFVRYLIFGNVLRGEVYPLSVAAERTQQA
                     ISVIEVAKKLGAKAVAHGSTGAGNDQIRFDVALRVLAPELEVVTPIRDEGVKRETSVS
                     YLRERGLPVPERATAYSVNRGLWGTTWGGGWTHDSWASPPQELLDPPNPSLAPRDVVI
                     GWEKGVPVSVDGKRLTGAQLVDVLGELCEQFGIGRNIHVGETVLGIKGRVGFEAGAAL
                     VLISAHRELEKLVQTKWQGFWKDHLGRFYGDRLHEGQYFDPALRDIEALLDSSQQRVT
                     GETRVRLAPGRYQVTGARSPASLLDRAVATYGEENLLWSGDEARGFARVSAVAPLLAA
                     RAQARAEALTAVEAAKKP"
     gene            3681..4757
                     /gene="carA"
                     /locus_tag="PKL17_10165"
     CDS             3681..4757
                     /gene="carA"
                     /locus_tag="PKL17_10165"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNK45138.1"
                     /translation="MKLCLADGTTLSGRSFGANVEARGEVVFHTGMVGYVEALTDPSY
                     RGQILVLTYPLIGNYGVPKEGMESSRIQVQGLVVSRYAPSHSHHLAVQSLADWLISSG
                     VPAVQGIDTRLLVQKLRSKGTVQGALVDEETLPQVGLAQAPGIAMRDAVDLVEPHSIV
                     ELPGSASGPTILVIDTGAKESIVRCLLARGARVVRAGASCAWEPYLSRVDGVLLTNGP
                     GDPRDQGPLVDRLKQVLARRLPTFGICLGHQLLARCLGADTYKLPFGHRSHNQPVLEV
                     GTRRAFITSQNHGYAVDEKTLPTDVAPWFVNLNDGTNEGIRHLSDPFSSVQFHPEAAS
                     GPRDTAPLFDDFMRCVAACRGSRP"
     gene            4754..8044
                     /gene="carB"
                     /locus_tag="PKL17_10170"
     CDS             4754..8044
                     /gene="carB"
                     /locus_tag="PKL17_10170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HNK45139.1"
                     /translation="MTMPSDVLSTLLAEKPELPKRVLVLGSGALQIGQAGEFDYSGSQ
                     ACKALREEGIYVVLLNPNIATVQTSEKLADAVYFLPVEPNIVEEVLVREQCDAILLGF
                     GGQSALNCGLRLSDEGILARHGVRVLGTSIETIRACEDRKIFADKLREIGVPVARGQV
                     ATSLAEAEKVAADIGYPLILRAGFSLGGRGSAIVAAPSDLKPAIERALAGVGQVLLEE
                     CLSGWKEIEYELVRDAADNCITICNMENFDPMGVHTGESIVVAPSQTLSDADYQLLRD
                     ASIATIRHLGIVGECNIQFALDPKASRYRVIEVNPRLSRSSALASKATGYPLAYVGAK
                     LQLGYLLPGLRNAITRTTTACFEPALDYIVCKVPRWDLEKFAGADLRIGTEMKSVGEV
                     MAIGRSFGEALQKALRMLEVGADGFDPNLLGLSKRDEVLAEISPPSSRRIFAIARAFA
                     IGFSVEEVAQQTAIDRFFLHELFELHTLRVGLRGLPLSSLPAALLRTCKQAGFSDAVI
                     AAQCGVKADDVRQRRKQLGILPKLRQIDTLAAEYPAQTNYLYLTYHADESDVLPSEKK
                     KLLLLGSGCYRIGSSVEFDWSCVGAAQAARELGYEVALLNCNPETVSTDYDLCDRLVF
                     DEVSLETVRELWDFEQGAGGGFVGVLLAMGGQTPNRLALPLSLAGIRLLGTSPQSIDC
                     AEDRAKFSALCDRLHVDQPRWTEAGKIEDLDGAVAAIGGYPVLVRPSYVLSGAAMRVA
                     HSGPELTLYLDGATQVTPEHPVVVSKYERHAREIELDAVVDHGKILLWAICEHIEDAG
                     VHSGDATMMLPPQDLTVEMVRKVKRIGAALCKSLDVTGPVNIQLLARDDEVKVIECNL
                     RASRSFPLVSKALGVDFIATATRAMLGEKVDLPKLRDPLDLDYVVVKAPMFSFRRLSG
                     ADPLLGVEMAATGEVGCFGDTVEEALAKSLLATGFRWPSRGVLLSLGTMGDKYLLVEE
                     ARELAKLGLLLYATPGSAETLHAEGIACHTVDKGESGSDSDVLRLLRSGAIDLVVNIP
                     RSFDSEGRPDGFLIRRAATDLEIPLLTDLQLARAVVRMLARVRPPAPGTTGTLRALPW
                     REYLARTN"
     gene            8115..9302
                     /locus_tag="PKL17_10175"
     CDS             8115..9302
                     /locus_tag="PKL17_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNK45140.1"
                     /translation="MSTTSTRQHLETLAIHAGMPPEPTTGAITPPVFMTSTYVQDGPG
                     GHKGYEYSRTHNPTRQALEANIAALEGGRFGVAFASGCAATTTILHTLSAGDHFVAGD
                     DLYGGTYRIFERVMKQLGVTATYVDPMRPGAFAEAILPSTKLVWIETPTNPLLKLCDI
                     AAVAQICQKAKLPLFVDNTFMSPVLQRPLELGATAVVHSTTKYINGHADVVGGVVVTS
                     DAALHDKLRFLQNAIGAVPGPMDCFLVARGVKTLPLRMERHCQNAQAMASWLESQIGG
                     GLIEKVIYPGLPSHPQHDLAKKQMHGAGGMISFVVGNGRLPALKRAENILRAVKLIAC
                     AESLGGVESLIEHPASMTHASIPVEQRNALGISDGLIRLSVGVEHIDDLMDDLKQAFA
                     QAE"
     gene            complement(9309..11390)
                     /locus_tag="PKL17_10180"
     CDS             complement(9309..11390)
                     /locus_tag="PKL17_10180"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNK45141.1"
                     /translation="MHDDVVLLLPGAAAPVSPDPALLGQKGAGLCQMAQRGLPVPPAL
                     ILPSTLWPEFQRTGSFPDSFWHQVEEKLALIGAKVDAQLGNPEKPLLLSVRSGAKVSM
                     PGMLETLLNVGVSEKTLPGLIARLGDERCALDCRRRFLHQYAALVYGLRPDTVLLPDP
                     LELLLFELRQTRGVTHDEELSADDLRGLCQKYQEFIAKRAGKPVPEDAFTQLREAVYA
                     VLRSFFSPRAEEYRRMQGIPSDIGTAVTLQAMVFGNLGPNSATGVVFSRDPRSGQKGL
                     YGEFLLEAQGDDLVSGGFTPRPIAELAPLLPDAYAQLDLAVQQLEQDLGDLQDVEFTI
                     ERGRLFLLQTRAGKRSARAMVKVACDLVREGRVSQEEAIRRCEPKRFAELLLPTVDPK
                     TSAPTIARGLPASPGAATGPVVFTAQDAVEQRRKGIGCILVRGETSTDDIVGIEAAAG
                     LLTTRGGMTSHAAVVARGMGRCAIVGCSTLKVDLARRTASTHAYTIRQGDLVTLDGHR
                     GLLLLGSLPLSLSHVHDDADVALLSNWAMATARVPIWARLHSDNDRTLARDLPISRLV
                     RLDPPSDGGIRTLHVETLLPQVPPQPGVLLPLSEANRLPQLTTEHSLIALETTTLPQD
                     DLAPTIAEARRVAGQKGVQIGLLVNEPLSVEPRLHALAISLKLGFVACPLLRTPLAWL
                     VAARTALDTTS"
     gene            complement(11394..11729)
                     /locus_tag="PKL17_10185"
     CDS             complement(11394..11729)
                     /locus_tag="PKL17_10185"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNK45142.1"
                     /translation="MSQKPLSHQSPTVDSASPTPEPDVDLQVEIFRTRNRMLAQMVVE
                     DILASEGVSAVLHDRQMHSLPAPLAMSGEIGVAVSEIDREVALSALREAQKNGVPLDE
                     GEILEESVG"
     gene            11917..14718
                     /locus_tag="PKL17_10190"
     CDS             11917..14718
                     /locus_tag="PKL17_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45143.1"
                     /translation="MKKPTPFGKYFLFDRVSTGGMAEIFRAKTVSLEGTERIVAVKKM
                     IDLLTADKELTSMFIDEAKLAVQLNHPGICQVVDWGKVGDSYFIAMEYVLGQDLRHLF
                     QRCKTQLPDGTPTMPLAQSCFVVMKLCEALDYAHNKQDSLGNPLSVVHRDVSPQNVLV
                     SYEGEVKLIDFGIAKASVTRETQTEAGVLKGKFAYMSPEQVRGDAVDRRSDVFSTGIV
                     LYELLTGERLFPPDQELHVLEKIKNVEVLPPTAYNRKIPEELDRIVMKALAGQPADRY
                     QTAMDLHADLQSFLYTSGEFFSRKDLAAWMKRVFRTEMDAEQNEIDAIRDTKVALPTT
                     GADSLQRARATLSMAALKADPAGGLRTSKETGSIAAVKATGAMKAFQPEPGAPNEWMS
                     TNAKTVKNASSPPRGPVPTTRPSKTAASATKLKPVTDKPSQPRVHEVPVEEDTAVDGP
                     MGAGKIAEPNFADDNLATKAFPQQDQAALKAQVQAAKRNEKAGKDGVVQSTQDAGDIE
                     TQQYKTVDDGKNERDPLEGGPTVAVPPGQRIPGNIEEIMEVSSPVMLDPSQPVPVPQP
                     EPTRSRAIPAVAPPPPLQMPDSLDVGPRDATQNKPQGSNTSALLLLLLLLALGGGAAA
                     YWFLLRPGTVVVTVSPDREFAVLVDGKPMPGMDSSPVILTLPPGTHAVSVQRSGYKSW
                     ADAVTVQPGETLRVSAGLAAQVQQTGGFTLVTDPPGAMVFLDGASLGQVTPMRVGSVP
                     VGTHSLELRLGARVLVQTITVEAGKSLDLKFPLPPEPTSKPAEPVVQKEPEKQPATQK
                     EPEKQPEKQPVAQKEPEKQPVAQKEPEKQPVAQKDPERHIAPRPQPRPTPQPTVQRPV
                     APRPAGNFGFLRLNSKPWTKIFVDGQDTNLNTPQTAIQMTPGMHQIMLYNPEFAIRET
                     FSVHIIAGETQTIIKDFRK"
     gene            14719..15552
                     /locus_tag="PKL17_10195"
     CDS             14719..15552
                     /locus_tag="PKL17_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK45144.1"
                     /translation="MSPNDPLSAPRFVLSATGQARGVLLVHGFSGSPYELHLLADRLV
                     DAGYSVALPMLRGHHQSLRALAESTWQDWLSSAEQALLGLHRRMWETTQKTPQLAVIG
                     FSMGGLLSLELARRYPAAPTFARPDLPMVRALSVLSSPLWLAPWQEKAIVRLADSGLR
                     GLAVPKLAGRDVRAKDLAKAPLRPWGMPVRALASLVQLMRTVRPRLPDVLQPALLAHG
                     QLDHTVPVACVDALADELGTLAEHKHKLVLSRSYHIVPLDVDREELFAAIDAHLARYL
                     D"
     gene            complement(15574..16644)
                     /locus_tag="PKL17_10200"
     CDS             complement(15574..16644)
                     /locus_tag="PKL17_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45145.1"
                     /translation="MRRLTLATLLLSGCFDWDVLSSNVVEPDLSVLDDLSVPADMSVA
                     DLSRADLAVPPDLTKPDMALPVTNVTKQADPTKFSGKKPNNAIFGWNTQNITAVGDGG
                     VVVTTTDGTNWSLVAISGGFTQNIQDVWFSGDGSTGWFVGAGTSAYQWTSAGNSWQSK
                     NAGLGGVLYSVFGTANDKVASAGDQNNKLYRFVTSWANDNNTPNYGSTQNGLWGTGAN
                     FYSVSSAGADCLLITDAAITKVSCGTNGTLETVWGVDTMNVFAAGYENNTGIVSKYGG
                     SSWKKEPIPTVGKLWSVWAAGSKDVWVGGEAATLLHYDGTSWTQHNAVGMGATETIKD
                     IWGADANNVWFLTDAGNIYKKN"
     gene            complement(16644..17411)
                     /locus_tag="PKL17_10205"
     CDS             complement(16644..17411)
                     /locus_tag="PKL17_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45146.1"
                     /translation="MLTSPRFFSALCLPLVCLVSLPTSWAADADPAEPGQAAATKKPL
                     PPPPPEALAAGRAFFEKGQAYFKAGKYQAAWVEFSSAYQIVEMPDLLFNIARCEAKMG
                     RPADAAKHFRQFLAAKPDDPEADNIRRQIDELEGRVANPPTGTSADKKPLQIPWIATA
                     TGGAGLLLVLGGAGALGAANSTYYSLQMECGTGCERSKTDSGRTQSAVGYSLFAIGFA
                     AMASAAVILPFELKKKDAASKVALSITPGGLAVLGRY"
     gene            17557..18288
                     /locus_tag="PKL17_10210"
     CDS             17557..18288
                     /locus_tag="PKL17_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK45147.1"
                     /translation="MLRIRAFLTSFFIIAYTIFMSVVAVVTWPLSPRGDLFLTFAKIW
                     SRVIFFVGGIRWVTQRSDKLDLQKSYLYLSNHESLTDILALYATLPQKAVMVAKRSLF
                     ILPVFSWGMWLAGFIFINRKNTKEAYARLQSAGKLIRKGRSVLVFPEGTRGDGGELLR
                     LKRGGFVLAQTAQIEVVPVGIAGTAEVLPRDTFPVYPGSIAVCIGEPMAPPRPDEDLP
                     RYMDRVRAEIDKLRAVAREQAAKNR"
     gene            complement(18373..19047)
                     /locus_tag="PKL17_10215"
     CDS             complement(18373..19047)
                     /locus_tag="PKL17_10215"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNK45148.1"
                     /translation="MKHASTLSNLLCGMLLIAAPLTASAGSDGAAASSKSKKARHPVF
                     SGWVASESQLRSRPAARPSGNIVISSVNEPKSQVTVNIYNPDGSFNEEALEKLNHVWR
                     CRRTQTEKQIDPHLFEVISHIYDHFQKPLTLVSGFRNQKKTTSFHYAGSASDLSVPGV
                     PDSRLKAFVESLDTGGMGIGIYPHAGFIHVDIRPEPSYRWIDNSPPGSSDMGQPKTSR
                     ERSPNS"
     gene            complement(19273..19731)
                     /locus_tag="PKL17_10220"
     CDS             complement(19273..19731)
                     /locus_tag="PKL17_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNK45149.1"
                     /translation="MGRVSWDEYFMDIAKVVSSRSTCARKQVGAVIVRDRTILSTGYN
                     GSIRGMPHCDEVGHMLESDHCVATIHAEQNAIIHAAKNGVAIDGSDLYVTASPCWSCF
                     KSIANAGVRRIVYGEFYRDARIFDVAGRLGIELHHLPTDSQANSAVDSQR"
     gene            20139..>20319
                     /locus_tag="PKL17_10225"
     CDS             20139..>20319
                     /locus_tag="PKL17_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45150.1"
                     /translation="MSKTYRCPVCSNEVDSAATICSNPACRAELSFCSHCRDITTYVL
                     VEERRGKTSRNLYRCD"
BASE COUNT         3892 a         6043 c         6442 g         3942 t
ORIGIN      
        1 gagccagcgc tgtccatttt catcaaacag ccacgatcct tggccgcgga cgggatggac
       61 cggaagctgt gcatacaccg gcagcagcgc cgacgaagaa cgttcattta acatgggaat
      121 gcccccacca tttgcagacc ggctgtttcg ggcagaccga gcgccaggtt catcgcttgt
      181 accgcttgtc cggcggcacc tttgagcaag ttgtcgatgg ccacggtgac ttgcacttcg
      241 cgtccgtcgt cgctttggca ggcgtgacag agcgcgtagc tgccgccaag tgcgtgggtc
      301 aggtcaggcg gttttttcag cacccgaacc agaggccgat gcgcatacgc cgtcgccagt
      361 gcttgtgcag ccgcttccgc agcgagtggt ttttcgacga ccagatgggc cgtcagataa
      421 atcccgcgca ccatcggtcc cgaatgggtg ataagccgcg ccgtcgattc ggctcgtccg
      481 gtccagcgac gcaggctttg caggatctcg gcttcgtgac ggtgcgaaaa cgacgaatag
      541 gcgaacaggt tgtgggcgcg ggccggatgg tgcgtcgtgt cgcgcggctt ggctccggct
      601 ccgcttgaac ccgtgacggc aaacagcgct ggaatcgatg cgccgatcag gtcacgaaat
      661 ggatacagcg cgagctgagc agcggtggca aaacagccgg gtgccgcaat tgctgtggcg
      721 ccacgcagct tcggaccttc gacatcacac aagccgtata cgaactgcga aagcagatcc
      781 ggcgacggat gcggtccgta atggcgcgca tacagcgccg ggtcagccac gcgaaaatcc
      841 gccgccagat cgatcagcag cggtggtttt ttctcccaca gtggtgcaag cagtctcgac
      901 agctcgccgt gctcgagcgc tgacatcacg acatcgacat cagcaaactg cgctacgagc
      961 tcgccaccga caaagcgcgc atcggacaat ggcagcagcg tcggatggac atcggcaatc
     1021 gctttgtgtt cttggctgcg gctgtgcgcc gtgactttgg cgacgtgcgg atgttgcaaa
     1081 agcagccgta gcagctctcc accaacgtag cctccggccc caagcacgag tactttcagc
     1141 gcagggttca cggcttgtcc tcggcacacg cagcggcggc ttttttgggg gcgctgtcaa
     1201 tggcccgccg caccaccgcg acaagtccgg cagcatcgcg acgggcgttg tgtgccggaa
     1261 cgctcaggtg cgatcggatg tagtcgagcc ccacttcatt gtccgtcgct ggacccgtca
     1321 gaacagtcgc cgcaatgccg tagtcacggc gcaggatttc gaccgcgccc cagcagccga
     1381 ccggatcggg ggcagcgacg acccacgccc ccgccgcacc gcgcagcgcc tggtcgccca
     1441 ggatgtcttg cacgccgtat tcccccagga tgccgtcgcc cagctcggcg atgatcacgt
     1501 cgggtttggc gtcggatagc cggttgaaaa tgcctttggc ggtggcggtc gtcacaccgg
     1561 cccgcgtcga tgccaagcca ctgtcggaaa atgccagtgc agtgatcgct ccggcatcga
     1621 gcatttgcag cgtatcgcgc atcagcgaca cgccggtcag cttgcacgcc gcgacgcgaa
     1681 ccccttggcg ggtcagtcca cgcacaagtt ccgtcgcagc gtaggttttg ccggtgttca
     1741 tgcaggtgcc ggcgacgtaa atgaccggga cgcggctgcg cagatcatcg gcggttggca
     1801 cggcaccttg gcggatgttg gcggggcgtc cgacgcgatc ggacagttcc gggaagctga
     1861 gaatcgcccc cagcacttcg gcgcggaacg gtggaccgag ctctggactt gccgacgtgc
     1921 agcgccccag cacgccgccc aggttgagta catgcacagt gtccccaacg gaaatgtgcg
     1981 aaggaacttc ccccgcgtag ccgtgcagcg cccgtcgatt gcccagcgta ccggccaaga
     2041 tatcgcccgc gcgcagcggc accatgcgac ccgtcagatc ctcgacggtg ttgtaggttg
     2101 acttgtcttc gaggatgcgc accgccagca cgaagccttc cgccgcggtg atttggtcgc
     2161 tgacgatgac atcgcgtcca agcagcgcgt ttttcgtcga tgaagcgatg cggtcaagcc
     2221 gcaccctggt ggtgagcatc atggcttttt cgcagcctcc accgccgtaa gcgcttcggc
     2281 ccgcgcctgc gcccgtgcag ccaaaagcgg agccaccgcc gacacccgcg caaaaccacg
     2341 cgcttcgtcg cccgaccaca gcaggttttc ttcaccataa gtcgccaccg cacggtcgag
     2401 cagcgacgcc ggactgcgtg cgccggtgac ttgatagcgc cccggagcca gccgcacccg
     2461 cgtttccccc gtcacccgct gctgactgct atcgagcagt gcctctatgt cgcgcagcgc
     2521 cgggtcaaag tactgtcctt cgtgcagccg gtcgccgtaa aaccgaccca agtggtcttt
     2581 ccaaaagcct tgccacttgg tctgcaccag cttttccagc tcgcggtggg cagaaatcag
     2641 cacaagcgcc gcaccggcct cgaaaccaac ccgccctttg attcccagca ccgtctcgcc
     2701 gacgtgaatg ttgcgcccga tgccaaactg ttcgcacagc tcgcccagca catcgacaag
     2761 ctgtgcgccg gtcagtcttt tgccgtcgac cgaaaccggc acgccttttt cccagccaat
     2821 caccacgtcg cgcggcgcaa ggctcgggtt tggtggatcg agcagctcct gcggcggact
     2881 cgcccacgag tcgtgcgtcc agccgccacc ccaggtcgtg ccccacagac cgcgattgac
     2941 cgaatacgcc gtcgcccgtt cgggaaccgg cagcccccgc tcgcgcagat agctgaccga
     3001 tgtttcgcgc ttgacccctt cgtcgcgaat cggtgtcacc acttccagtt ccggcgccag
     3061 cacccgcagc gcaacgtcga aacgaatctg gtcgttgccc gcgcccgtcg agccgtgcgc
     3121 caccgctttg gctcccagct ttttggccac ctcgatgacc gaaatcgcct gctgggtccg
     3181 ttctgccgcc accgacaggg gatagacttc gccgcgcagc acgttgccga aaatcagata
     3241 gcggacaaag cggtcaaaca cctgctcgcg cgcgtcgacc gcatggtgag atgcggcgcc
     3301 gaccgcgtgg gcttgcttgg caatcgcttc gcgctggtcg ctgctggtgc cgccggtatc
     3361 gacaaacact gtgtgaactg tccagccggc ttcgctgagc ttggggacgc agtaagaagt
     3421 atcgagtccg cccgaaaagg cgagcgccaa tgggccccgt tttgtttgca taatgcatat
     3481 acatgcacaa agcgtttttg acgcacaaga actatttttt ggcctcgcaa tggctgagct
     3541 tgattggtcc aatcaaaagt tcgtcgaagt tgtctggttt ttccctttga cttggcgcgt
     3601 caactcgttc accctggcgg gccgtgaggt ctggctgtgg acagttgtaa gatgcataat
     3661 tgcatatttg gagccgcgtc atgaagctct gtttggcgga tggcacgacg ctttccgggc
     3721 ggtcgtttgg cgcgaacgtg gaagcgcgcg gcgaagtggt ctttcacacc ggcatggtcg
     3781 gctatgtgga agcgctcacc gacccttcgt atcgaggaca aatcctggtg ttgacctatc
     3841 cgctgattgg caactatggg gtgccaaaag aggggatgga gtcgtcgcgg attcaggtcc
     3901 aaggtctggt ggtgagccgt tacgcaccgt cgcacagcca tcacttggcg gtgcaaagcc
     3961 tggcggactg gctgatttcg tctggcgtgc cggcggtgca gggcattgat acccggctgt
     4021 tggtgcaaaa gctgcgcagc aaaggcaccg tgcagggcgc gcttgtcgac gaagaaacct
     4081 tgccgcaggt cgggcttgcg caggcgccgg ggattgcgat gcgcgacgcc gtggatctgg
     4141 tcgaaccgca cagcatcgtt gagctgccgg gttccgcatc gggtccgacg atcctggtga
     4201 ttgataccgg tgccaaagag tcgatagtgc gctgtctgtt ggcgcgtggg gcccgcgtgg
     4261 tccgagccgg tgcgagctgc gcgtgggaac cgtacctatc gcgcgtcgac ggtgtgcttc
     4321 tgacaaacgg gcctggcgat ccacgcgatc agggtccgct cgtcgacagg ctaaagcaag
     4381 tgctggcccg gcggctgccg acgtttggga tttgtcttgg ccaccagctt ttggcgcgct
     4441 gcctcggggc tgatacctac aagctgccgt ttggccatcg ctcgcacaac cagccggtgc
     4501 tggaagtcgg gacgcgacgg gcgtttatca ccagccaaaa tcacgggtat gccgtcgatg
     4561 aaaagacgct gccgaccgac gtggcaccgt ggtttgtcaa cctgaacgac ggcacaaacg
     4621 aagggattcg ccacctttcc gatccgtttt cgtcggtgca gtttcaccct gaagcggcgt
     4681 cgggaccgcg tgataccgcg cctttgtttg atgactttat gcgctgtgtc gctgcctgcc
     4741 gcgggagccg cccatgacca tgccatcgga tgttttgtcg acgctgcttg ccgaaaaacc
     4801 agagctgccc aagcgggtgc tggtgctcgg gtctggggcg ctgcaaatcg gtcaagcggg
     4861 cgagttcgat tactcgggat cgcaggcctg caaagcactg cgcgaagaag gcatttatgt
     4921 cgttcttttg aacccgaaca tcgcgacggt gcagacgtcg gaaaagctcg ccgatgcggt
     4981 ctattttttg ccggtcgagc caaacatcgt cgaagaagtg ctggtccgcg agcagtgtga
     5041 cgccatcctg ctcggctttg gcggacagag cgcgctaaac tgcggtctgc gactgtccga
     5101 cgagggaatc cttgcgcggc acggtgtgcg ggtgctcggc acgtcgattg aaacgatccg
     5161 cgcctgtgaa gaccgcaaaa tcttcgccga caagctgcgc gaaatcggag ttcccgtcgc
     5221 tcgtggccaa gttgcaacct cgcttgccga agccgaaaaa gtggccgcag acattggcta
     5281 tccgctgatt ttgcgcgccg gtttttcgct gggtgggcgt ggatcagcga tcgtcgctgc
     5341 gccaagtgat ctcaagccgg cgatcgagcg agcgctggcc ggcgtcggac aagtgctgct
     5401 cgaagagtgc ctcagcggct ggaaagagat cgaatacgag ctggtccgcg acgctgccga
     5461 caactgcatc accatctgca acatggaaaa cttcgatccg atgggcgtgc acaccggcga
     5521 atcaatcgtc gtggcaccgt cgcagacgct atcggatgcg gactatcagc tgttgcgtga
     5581 tgcgtcgatt gcgacgattc gccacctggg catcgtcggt gagtgcaaca tccagtttgc
     5641 gctcgatccg aaagcgtcgc gctatcgggt catcgaggtc aatccgcggc tgtcccgttc
     5701 gtcggcgctg gcttcgaaag cgacgggata tccgctcgct tacgtcggtg ccaagctgca
     5761 actgggctat cttttgccgg ggctgcgcaa cgcgatcacg cgcacgacga ccgcgtgttt
     5821 tgaaccggcg ctcgattaca tcgtctgcaa agtgccgcgc tgggatctgg aaaagttcgc
     5881 cggggccgat ctgcgaattg gcaccgaaat gaagtcggtc ggcgaagtca tggcgattgg
     5941 tcgcagcttc ggcgaagcgc tgcaaaaagc gctgcggatg ctggaagtcg gagccgacgg
     6001 ctttgatccg aacctgctcg ggctttcgaa gcgggacgaa gtgctggccg agatatcgcc
     6061 accgtcgtcg cgtcgtatct ttgccatcgc gcgggcgttc gcaatcggtt tttccgtcga
     6121 agaagtcgcg caacaaaccg caatcgatcg tttctttttg catgaactgt tcgagctgca
     6181 caccctgcgc gtgggtctgc gcggactgcc gctttcgtcg cttcccgcgg cgctgcttcg
     6241 cacttgcaag caagcgggtt tttccgacgc ggtgattgcg gcgcagtgcg gcgtgaaagc
     6301 ggacgatgtt cgtcagcgac gaaaacagct cgggattttg ccgaagctgc ggcagataga
     6361 cacgctggcg gcggaatatc cagcgcagac caactacctg tacctgacct atcacgccga
     6421 cgaaagcgat gtgctgccgt cggaaaaaaa gaaactgctg ctgctcgggt cgggctgtta
     6481 tcggatcgga tcgtcggtgg aatttgactg gagctgtgtc ggggcggcgc aagccgcgcg
     6541 tgagctgggc tatgaagttg cgctgctcaa ctgcaatccc gagacggtga gcaccgacta
     6601 cgatctgtgc gaccggctgg ttttcgacga ggtctcgctc gaaacggtgc gcgagctgtg
     6661 ggattttgaa cagggcgcag gcggtggttt cgtcggtgtg ctcctggcga tgggcggtca
     6721 gacgccgaat cggctggcgc tgccgctgtc gcttgcgggg attcgtttgc tggggacgtc
     6781 gccgcagtcg atcgactgtg ccgaagatcg cgccaaattt tccgcgctgt gcgaccggct
     6841 gcacgtcgat cagccgcgct ggaccgaagc gggaaagatc gaagacctcg acggtgcggt
     6901 ggcggcgatt ggcggctatc cagtgctggt tcgtccgtcg tatgtgctgt cgggcgccgc
     6961 gatgcgggtg gctcacagcg ggccggagct gacgctgtat ctggacggtg cgacgcaggt
     7021 gacgccggaa catccggtcg tcgtcagcaa atacgaacgg catgcgcgcg aaatcgaact
     7081 cgatgccgtc gtcgaccacg ggaagatcct gctgtgggcg atctgcgagc acatcgaaga
     7141 cgcgggcgtt cactcgggcg atgcgacgat gatgctgccg ccacaagacc ttacggtcga
     7201 aatggtccgc aaagtgaagc ggattggcgc tgcgctgtgc aaatcgctcg atgtgacggg
     7261 accggtcaac atccagcttt tggcgcgcga cgatgaagtc aaagtcatcg agtgcaacct
     7321 gcgggcgtcg cgcagctttc cgctggtgtc gaaagcgctc ggggtggact tcattgcaac
     7381 ggcgacgcgg gcgatgttgg gcgaaaaagt cgatctgccc aagctgcgcg atccgctcga
     7441 tctcgactat gtggtcgtca aagcgccgat gttttcattc cgaaggctgt cgggtgcgga
     7501 tccgctgctc ggggttgaaa tggccgcgac gggggaagtc ggttgtttcg gcgataccgt
     7561 cgaagaagcg ctggccaaat cgctgctcgc gacgggcttt cgttggcctt cgcgtggcgt
     7621 gcttttgtcg ctggggacga tgggcgacaa atatcttctc gtcgaagaag cgcgcgagct
     7681 ggcgaagctg gggcttttgc tgtacgcgac accagggtct gccgaaacgc tgcatgcgga
     7741 aggaattgcg tgtcataccg tcgacaaagg cgaaagtggc agcgattcgg acgtgttgcg
     7801 gcttctgcgc tcgggcgcga ttgatttggt cgtcaacatc ccgcgcagct tcgacagcga
     7861 aggtcgtccc gacggctttt tgattcggcg cgcggcgacg gatttggaaa ttccgctgct
     7921 caccgacttg cagcttgcgc gggcggtggt gcgaatgctg gcgcgggtca ggccgccggc
     7981 gccgggaacc acgggaacgc tgcgcgccct gccgtggcgt gagtatttgg cgcgcacaaa
     8041 ctaagccaat ccggcctcgc tggcagcggc gccgggtttg gggtagcgtg cctgcactgc
     8101 gaaggaaatc tacgatgagc acgacttcta cacgccagca ccttgagacc ctcgcgattc
     8161 atgccggaat gccgcccgag ccgacgacgg gcgcgattac gccgccggtt ttcatgacgt
     8221 cgacgtatgt gcaagacggg ccgggcggac acaaaggcta cgaatattcg cggacccaca
     8281 atccgacgcg gcaagcgctg gaagcgaaca ttgcggcgct cgaaggaggc cgtttcggcg
     8341 tggcgtttgc gtcgggctgc gcagcgacga caacgatcct gcacacgctt tcggcgggcg
     8401 atcacttcgt cgcgggtgac gatctgtacg gcggcaccta tcgaattttt gaacgggtga
     8461 tgaagcagct cggggtgacg gcgacgtatg tcgatccgat gcggcccggt gcatttgccg
     8521 aagcgatttt gccaagcacc aagctcgtct ggatcgagac gccgaccaat ccgctgctga
     8581 agctgtgcga catcgccgcg gtggcgcaga tttgccaaaa ggccaagctt ccgctgttcg
     8641 tcgacaacac cttcatgtcg ccagtgctcc agcgtccgct ggaactgggc gcgacggcgg
     8701 tggttcactc gacgaccaaa tacatcaacg ggcatgccga tgtcgttggt ggtgtcgtgg
     8761 tgacgagcga cgcggcgctg cacgacaagc tgcgcttttt gcaaaatgca atcggggcgg
     8821 tgccgggacc gatggactgc tttttggtcg cgcgcggggt caagacgctg ccgctgcgga
     8881 tggaacggca ctgtcagaat gcgcaggcga tggccagttg gctcgagtca cagatcggcg
     8941 gcggtctgat cgaaaaggtg atttatccgg gtttgccgtc gcatccgcag cacgatttgg
     9001 ccaaaaagca gatgcacggg gcgggcggca tgataagttt tgtcgtcgga aacgggcgtc
     9061 tgccggcgct caagcgggcg gaaaacatcc tgcgtgcggt caagctgatt gcctgcgcgg
     9121 aaagcctggg tggcgtcgaa tcgctgattg agcacccggc gagcatgacc cacgcgagca
     9181 ttcccgtcga acagcgcaat gcgctcggca tttccgacgg cctgattcgt ttgagcgtcg
     9241 gcgtcgagca catcgacgat ttgatggacg atctgaaaca ggcgttcgcg caggcggaat
     9301 aaagtggtct agctcgtcgt gtcgagcgcc gtgcgcgccg cgacgagcca agcgagcggt
     9361 gtgcgcaaaa gcgggcaggc gacaaagcca agcttgagcg aaatcgcgag cgcatgcaga
     9421 cggggctcga cggacagcgg ttcatttacc aacagtccta tctgcacgcc tttttgacct
     9481 gccacccgtc gggcttcggc gattgtcggc gccaagtcgt cttgcggcag cgtcgtcgtt
     9541 tccagcgcaa tcagcgaatg ttccgtcgta agctgcggca gccgattggc ttcggacagc
     9601 ggcaagagca caccgggctg cggcggcact tgcggcagca gcgtttcgac gtgcagcgtt
     9661 cggatgccgc catcggacgg tgggtccagc cggaccagac gtgaaatcgg cagatcgcga
     9721 gccagcgttc ggtcattgtc gctgtgcagt cgcgcccaaa tcggcacgcg cgccgtcgcc
     9781 atcgcccagt tcgacaacag cgcgacatcc gcatcatcgt gaacgtgcga aagcgaaagc
     9841 ggcagtgatc cgagcagcag cagaccgcgg tgtccgtcga gcgtaaccaa atcaccctgc
     9901 cggatcgtgt aggcgtgcgt cgatgcggtt cgccgggcca gatcgacttt gagcgtggag
     9961 cagccgacga tggcgcagcg acccatgcca cgcgcgacga ccgccgcgtg cgacgtcatg
    10021 ccaccgcgcg tggtcaaaag tcccgccgcc gcttcgatgc caacaatgtc atccgtcgat
    10081 gtttcgccac gcaccaggat gcagccaatt cctttgcgtc gctgctcgac ggcatcttgt
    10141 gcagtaaaga cgaccggtcc ggtggcggca ccaggcgacg ccggcagacc gcgcgcaatc
    10201 gtcggtgcac tggtcttcgg atcgacagtc ggcagcaaca gttcggcaaa acgcttcggt
    10261 tcacagcgtc ggatcgcttc ttcctgactg acccggcctt cgcgcaccaa gtcgcaggcc
    10321 actttgacca tcgcccgcgc cgagcgcttg cccgcccgcg tttgaagcag aaacagccgc
    10381 ccgcgctcga tggtgaactc gacgtcttgc aggtcgccca agtcctgttc aagctgttgc
    10441 accgccaagt caagctgcgc atatgcgtcg ggcaaaagcg gcgccagctc ggcaatcggg
    10501 cgcggcgtaa aaccacccga caccagatcg tcgccctgtg cttcgagcag aaattcgccg
    10561 tacaggcctt tttggccact gcgcgggtcg cgcgaaaaca ccacacccgt cgcactgttt
    10621 ggtcccaagt tgccaaacac catcgcttgc agcgtcactg cggtgccgat gtccgacgga
    10681 atgccttgca tccgacggta ttcttcggcg cgtggtgaaa aaaacgatcg cagcaccgca
    10741 tagacggcct cccggagctg tgtaaacgca tcttcgggga cgggcttacc ggcgcgcttg
    10801 gcgataaatt cttggtattt ttgacacagt ccgcgcaggt cgtcggcgga cagctcttcg
    10861 tcgtgggtga cgccgcgggt ctgccttagc tcaaacagca gcagctcgag cggatcgggc
    10921 aaaagcacgg tgtccggcct gagcccatag acgagcgccg catattggtg caaaaaccgc
    10981 cgtcggcagt ccagcgcaca gcgttcatcc ccaagccgcg caatcagacc cggcagcgtc
    11041 ttttcgctta caccgacgtt gagcagcgtt tccagcatgc ccggcatcga cactttggca
    11101 cccgagcgca ccgacagaag cagcggcttt tctgggttgc caagctgtgc atcgaccttg
    11161 gcaccgatga gcgccagctt ttcttcgact tggtgccaaa acgaatcggg aaaactgccg
    11221 gtgcgctgaa attctggcca cagcgtcgac ggcagaatca gcgctggcgg cactggcaag
    11281 ccacgctgcg ccatctgaca cagcccagcc cctttttgcc caagcagcgc cggatcgggg
    11341 gagacgggag ccgcagcacc gggcagaagc agcaccacgt cgtcgtgcat gacttaacct
    11401 accgattcct cgaggatttc cccttcatcg agcggcacac cgtttttttg cgcttcccgc
    11461 agcgctgaaa gtgcgacttc gcgatcgatt tcggacaccg cgacgccgat ttcccccgac
    11521 atcgcgagcg gcgccggcag cgagtgcatc tgcctgtcgt gcaggaccgc cgagacacct
    11581 tccgacgcca agatgtcttc gacgaccatc tgcgcgagca tgcgattgcg ggtacggaaa
    11641 atctcaacct gcaaatcgac atccggttct ggcgtgggcg acgccgaatc gactgtcgga
    11701 ctttggtgcg agagcggctt ttgcgacatg tgcctaaagg agctaacacg gccagcctac
    11761 gcggtccagc cgcttgcaaa aagccaagca agcgaaagcg acaataccga gttctgcatt
    11821 gtgaatcgct aagatgcgat gctaacctgc ctattggtgc acactagctc agctcgcttt
    11881 gcagcaagct gaccccttct tgtgccgagg atgcttgtga aaaagcctac tccgtttgga
    11941 aagtacttcc tcttcgaccg cgtcagcacc ggcgggatgg ccgagatctt ccgtgcgaag
    12001 accgtctctc tcgaaggtac cgaacgcatc gtcgcggtca agaagatgat cgacctgctc
    12061 accgccgaca aagagctgac ttcgatgttc atcgacgaag ccaagttggc ggtgcagctt
    12121 aaccaccctg gcatctgtca ggtcgtcgac tggggcaagg tcggcgacag ctacttcatt
    12181 gcgatggaat atgtgctcgg gcaagacctg cggcacctgt ttcagcgctg caagacgcag
    12241 ctacccgacg gaacgccgac gatgccgctg gcgcagagct gctttgtggt gatgaagctg
    12301 tgcgaagcgc tcgactacgc ccacaacaag caggactcgc tgggcaatcc gctcagcgtc
    12361 gtccatcgcg atgtttcgcc gcagaatgtg ctggtcagct acgaaggcga ggtcaagctg
    12421 atcgactttg gcatcgccaa agcgtcggtg acgcgcgaaa cccagacgga agccggcgtg
    12481 ctcaagggca agttcgcgta catgtcgccc gagcaggtgc ggggcgatgc ggtggaccgc
    12541 cgatccgacg tgttttcgac gggcatcgtg ctgtatgaac tgctgactgg cgagcggctg
    12601 tttccgcccg accaagagct gcacgttctg gaaaaaatca aaaacgtcga agttctgccg
    12661 ccgaccgcct ataaccgcaa gatccccgag gagctggatc gcatcgtcat gaaggcgctg
    12721 gcaggccagc cggcggatcg ttatcagacg gcgatggatc tgcacgcgga tcttcagtcg
    12781 ttcctctaca cgtcgggaga gttcttttcg cgcaaggatc tggcggcgtg gatgaagcgg
    12841 gtgttccgca ccgaaatgga cgccgagcag aacgagatcg acgcgatccg cgacaccaaa
    12901 gtggcgttgc cgacgacggg cgcggactcg ctccagcggg cgcgggcgac gctgtcgatg
    12961 gcggcgctca aggccgatcc ggcgggcgga ctgcgcacgt cgaaagagac cggcagcatc
    13021 gccgcggtca aggccactgg cgcgatgaag gcattccaac cggagccggg tgcgccgaac
    13081 gagtggatgt cgacgaacgc caagacggtg aaaaacgcaa gctcgccgcc gcgtgggccg
    13141 gttccgacga cccgtccgag caagacggca gccagtgcta ccaagctcaa gccagtcacc
    13201 gacaagccgt cgcagccgcg ggtccatgaa gttccggtcg aagaagacac cgccgtcgac
    13261 ggaccaatgg gcgctggcaa gatcgccgag cccaactttg cggacgacaa cctcgcgacc
    13321 aaggcgtttc cgcagcagga tcaagcggcg ctgaaagcgc aggtgcaggc ggcgaagcgg
    13381 aacgaaaaag ccggcaaaga tggcgtcgta cagtcgacac aagacgctgg cgacatcgag
    13441 acgcagcagt acaaaaccgt cgatgacggc aaaaacgagc gcgatccact tgaagggggt
    13501 ccgaccgtcg ccgtaccgcc agggcagcgg attccgggca acatcgaaga gatcatggaa
    13561 gtcagctcgc cggtgatgct cgatccgagc cagccggtgc cagtgccgca gcccgagccg
    13621 acccgctcgc gtgcgattcc tgcggttgcg ccgccgccgc cgcttcagat gccggactcg
    13681 ctggatgtcg ggccgcgcga tgcgacgcag aacaaaccac aagggtcgaa cacgtcggcg
    13741 ctgttgcttc tgcttttgct gttggctctc gggggtggcg cggcggcgta ctggttcctg
    13801 ctgcgtccgg gcacggtggt ggtgacggtc agtcccgatc gggaatttgc cgtgctggtc
    13861 gatggaaagc cgatgccggg gatggactcg tcgccggtga tcttgacgct gccgccaggg
    13921 acgcacgcgg tgtcggtgca gcgcagcggc tacaagtcgt gggctgatgc tgtgacggtg
    13981 cagcccggcg agacgctgcg ggtgtcggcg gggcttgcgg cgcaggtgca gcagaccggc
    14041 ggctttacgc tggtcacgga tccgccggga gcgatggtgt ttttggatgg tgcttcgctt
    14101 ggccaggtga cgccgatgcg ggttggcagt gtgcctgtcg gtacgcactc actggagctg
    14161 cggctggggg cgcgggtgct tgtgcagacc atcacggtgg aagcgggcaa gtcgctggat
    14221 ctgaagttcc cgctgccgcc cgagccgacc agcaagccgg cggaacctgt ggtgcagaaa
    14281 gagccagaaa agcagcctgc tacgcagaaa gagccagaaa aacagccgga gaagcagccg
    14341 gttgcgcaga aagagccaga aaaacagcca gttgcgcaga aagagccgga aaaacagccg
    14401 gttgcgcaaa aagatccgga gcgacacatc gcgccacgtc cgcagccacg gcccacgccg
    14461 cagccgacgg ttcagcgccc ggtggcgccg cgacctgctg gcaactttgg ctttttgcgg
    14521 ctcaattcca agccgtggac caagatattt gtcgacggtc aggacaccaa cctaaacacg
    14581 ccgcagaccg cgattcagat gacgccgggg atgcaccaga tcatgctgta taaccccgag
    14641 tttgcgattc gcgagacgtt ctcggtccac atcattgcgg gcgagacgca gaccatcatc
    14701 aaagacttcc gcaaataaat gagcccaaac gacccgctgt cagcaccgcg ctttgtactt
    14761 tctgcgacgg ggcaggcgcg gggcgtgctg ctcgtgcatg ggttttccgg atcaccgtat
    14821 gaactgcacc tgctcgccga ccggctggtc gacgcgggct attcggtggc gctgcctatg
    14881 ctgcggggtc atcaccagag cttgcgggcg ctcgcggaga gcacctggca agactggctg
    14941 tcatcggcag aacaagcgct gctcgggctg catcggcgga tgtgggaaac gacccaaaag
    15001 acgccgcagc ttgcggtgat tggtttttcg atgggcggcc tgctcagcct tgagctggct
    15061 cgtcgctatc ccgcggcccc gacgtttgcg cgcccagatt tgccgatggt gcgggcgcta
    15121 tcggttctgt catcgccgct gtggcttgcg ccgtggcaag aaaaagcgat tgtgcggctg
    15181 gcggattcag gactgcgcgg gctggcggtg ccaaagctgg cggggcggga tgtgcgggcc
    15241 aaagacctgg cgaaagcgcc gctgcgtccg tgggggatgc cggtgcgggc actggccagc
    15301 ctggttcagc tgatgcgcac ggtgcgaccg cggcttcccg atgtcttgca gccggcgctg
    15361 cttgcgcacg gtcagctcga tcacacggtg ccggtcgctt gtgtcgatgc gctggccgat
    15421 gagcttggca cgttggccga gcacaaacac aagctggtgc tttcgcgctc gtaccacatc
    15481 gtgccgctgg atgtagaccg cgaagagctg ttcgccgcga tagacgccca cctggcccgc
    15541 tatctggact aggtggcgat cgaaacgtgc ggactagttt ttcttgtaaa tattgccggc
    15601 gtcggtgaga aaccagacgt tgttggcgtc cgcgccccag atgtctttga ttgtttcggt
    15661 ggcacccatg ccgaccgcat tgtgttgggt ccagctggtg ccgtcgtagt ggagcagcgt
    15721 cgccgcttca ccgccgaccc agacatcctt gctacctgcc gcccacacgc tccagagctt
    15781 gccgacggtg ggaatgggct cttttttcca gctgctgccg ccgtatttgc tgacgatgcc
    15841 ggtgttgttt tcgtagcccg cggcaaacac gttcatggtg tcgacgcccc agaccgtttc
    15901 cagggtgccg ttggtcccgc agctcacctt ggtgatggcc gcgtcggtga tcaacaggca
    15961 gtcggcgcca gcgctgctta ccgaatagaa gtttgcgccg gtgccccaca ggccgttttg
    16021 cgtcgatccg tagttcggcg tgttgttgtc attggcccag ctggtgacga agcggtagag
    16081 cttgttgttt tggtcaccgg cgctcgccac tttgtcgttg gccgttccaa acactgaata
    16141 caagacgccg cccaggccgg cgtttttcga ctgccagctg ttgccggcgc tggtccactg
    16201 atacgcgctg gttccggcac cgacgaacca gccagtcgat ccgtcgccgg aaaaccagac
    16261 atcttggatg ttctgggtaa agccgccact gatggcgacg agtgaccagt tggtgccgtc
    16321 tgtggtggta acgacgacgc cgccatcgcc gaccgccgtg atgttctggg tgttccagcc
    16381 gaaaatggcg ttgttgggct ttttgccgct gaacttggtg gggtcggctt gtttggtgac
    16441 gtttgtgacc ggcaacgcca tgtcgggctt ggtcaagtcg ggcgggacgg ccaagtcggc
    16501 gcgcgacaga tcggcgacgg acatgtccgc aggcaccgag agatcgtcga gcactgacag
    16561 atccggctcg acgacatttg acgaaagcac gtcccagtca aagcagccag aaagaagcag
    16621 cgtcgcaaga gtcagacggc gcattagtac ctcccgagga cggcgagccc acctggcgta
    16681 atcgacaggg caactttgct tgcagcgtcc ttctttttaa gctcaaacgg caagatcacc
    16741 gcggcgcttg ccatcgccgc aaagccgata gcaaaaagtg aataaccaac ggcagattgg
    16801 gtgcgcccgc tgtcggtttt cgaccgctca cagccggttc cgcactccat ttgcagcgag
    16861 taatacgtcg agttggctgc gcccagggcc cccgcgccac ctagcaccag cagcagacct
    16921 gccccgcctg ttgccgtcgc gatccacggg atttgcagcg gctttttgtc tgcactcgtg
    16981 cctgtcggtg ggtttgcgac gcggccttct agctcgtcga tttggcgacg gatgttgtcg
    17041 gcttctggat catcgggctt ggccgccaaa aactggcgaa agtgcttcgc cgcatcggct
    17101 ggccgaccca tcttggcctc acagcgagcg atgttaaaca gcagatcggg catctcgacg
    17161 atttggtacg ccgagctgaa ctcgacccac gcagcctggt acttgccggc tttgaaatac
    17221 gcttggccct tttcgaaaaa ggcgcgtcca gccgcgagcg cttccggggg cggcggcggc
    17281 agcggctttt tggtggcagc ggcttgccct ggttcggcgg ggtcggcgtc ggcagcccaa
    17341 gaagtcggca agctgaccag gcagaccagc ggcaaacaga gcgcggagaa aaatcgaggg
    17401 gaagtcaaca tcacgtactc gagtagttca tattgtaatt tgaaatccaa taaaactcag
    17461 atgaaatgaa ggctctatcg tcggaccagc gcgttcgaga agccagcctc gaaaggatag
    17521 acggttgacc aaggctgagg cattctgtgc gccccgatgc tcagaatccg cgcctttctc
    17581 accagttttt tcatcatcgc ctacacgatt ttcatgtcag tggtcgccgt cgtgacgtgg
    17641 cccctgtcgc cacgcgggga tctgtttctg acgtttgcca agatttggtc gcgggtcatt
    17701 tttttcgtcg gtgggattcg ctgggtgacg cagcgcagcg acaagctcga tctgcaaaaa
    17761 agctatctct acctgtccaa tcacgaatcg ctgaccgaca tcctggcgct ctacgcgacg
    17821 ctgccgcaaa aggccgtgat ggtggccaag cgctcgctgt tcatcttgcc ggtgtttagc
    17881 tgggggatgt ggctcgcggg cttcattttc atcaatcgca agaacaccaa agaggcctac
    17941 gcccgtctgc aaagcgcagg aaagctgatc cgcaagggac gttcggtgct ggtgtttccc
    18001 gaaggcacgc gcggcgatgg tggtgagctg ttgcggctca aacgcggtgg tttcgtcctg
    18061 gcgcagacgg cccagatcga ggtggttccc gtcggcattg ccgggacggc ggaggttctg
    18121 cctcgcgaca ccttcccggt ctatccgggc agcatcgcgg tgtgtatcgg ggaaccgatg
    18181 gcgccgccac gtcccgacga agacttgccc cggtacatgg atcgtgtgcg ggccgaaatt
    18241 gacaagctac gcgccgtcgc gcgcgagcaa gcggccaaaa accgctagcc agccggtcgc
    18301 gcaaagacgg tttgggaagg attgctgttg cggcgcagcg tttgtgtggt gccgcgtcgg
    18361 gaaggtcggt gtttacgaat tcggcgagcg ctcacgcgag gtcttgggct gacccatatc
    18421 gcttgagcct ggcggcgaat tgtcgatcca gcgatagctg ggctcggggc ggatgtcgac
    18481 gtgaatgaag cccgcgtgcg gataaattcc aattcccatc ccgccggtgt cgaggctttc
    18541 gacgaatgct ttgagacggg aatccggcac accaggcacg ctcaaatcgg acgcgctgcc
    18601 ggcgtagtgg aacgacgtgg tcttcttctg gttgcggaag ccggaaacca gcgtcagcgg
    18661 cttctggaag tgatcgtaga tgtgagagat gacctcgaac agatgcggat cgatctgttt
    18721 ttcggtctgg gtgcggcgac agcgccacac atggttcagc ttttcgagcg cttcctcgtt
    18781 gaagctgcca tcggggttgt agatgttgac cgtgacctgg ctcttgggtt cgttcaccga
    18841 cgagatgacg atgtttcccg acggacgagc ggctgggcgg ctgcgaagct gtgactccga
    18901 ggcaacccac cccgaaaaca ccggatggcg ggctttcttc gacttggagg aagcagcggc
    18961 accgtcagag cctgcactgg ccgtgagcgg cgcggcgatg agcaacatcc cacacaggag
    19021 attcgagagt gtgctcgcgt gtttcactgt tccattcctc cgttgactgc gagttgaaaa
    19081 agacaatttc gtaaacagtt cagagccgct ttgtgttccg aagaaaacct cgttcctagg
    19141 ccaagaatag gaggcaacgc caaagaaccg aaccccaccg tagctttggc aaagcgagtg
    19201 ggtcaacaaa aaacgtccgc tattccgctg atttcttcga caccacagcg acggattttc
    19261 ccttgcatct ctctatcttt gggagtccac agcggaattg gcttgggagt ccgtagggag
    19321 atggtgaagt tcaattccca gacgccctgc gacatcgaaa atccgcgcat cccgatagaa
    19381 ttcaccgtat acgatgcggc gaacacctgc gtttgcaatc gacttgaaac aagaccagca
    19441 cggcgacgcg gtgacgtaca aatcggaacc atctatcgcg actccgtttt tggccgcatg
    19501 gatgatggcg ttctgttccg catgaatcgt ggcgacgcag tgatccgatt cgagcatgtg
    19561 gccgacctca tcacagtgcg gcatgccgcg aatcgatccg ttgtagccag tcgacaagat
    19621 ggttcggtcg cgaacgataa cggcgccaac ttgcttgcgc gcacaggtcg atctcgacga
    19681 caccactttg gcgatgtcca tgaagtactc atcccaagag acacgaccca tggcgtcttt
    19741 cctttgcggt ccgcggcgtc acgacgacac tcccttcgac ttgggaatac cggtacagag
    19801 tcttcctgac agcgtagaca gagccctcct tgcggacaaa ggagtcttcc caagagagca
    19861 cggaaagact cccaatcggc aagcagagtc aattcgtttt ccgtcgctgc ggtttttagg
    19921 gaatgccatg ggcctggatc tgtttgggct gggaatgtgg tcggtcgcgt ggtggttgag
    19981 gtagctattt cttcgaagaa ggcagaaaca aaaacggctc ttattgtttt ccataggaat
    20041 gaatcgctgg gaagtcaagc gctcattctg cgggccgagg gaattcgtat cgggaagcag
    20101 tttgcagacg ggagcaaaga gcgtggtatc ttgaatgtat gagcaagacc tatcgctgcc
    20161 ctgtctgctc gaacgaagtc gactctgcgg cgacgatttg ttcaaatcca gcctgtcgcg
    20221 ccgagctttc gttctgttcc cactgtcgcg acatcaccac ctacgttctt gtcgaggaac
    20281 ggcggggcaa aacgtcgcgc aatttgtatc gctgcgacc
//
LOCUS       DAORGN010000075        20220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1044_length_20220_cov_5.938557, whole genome shotgun sequence.
ACCESSION   DAORGN010000075 DAORGN010000000
VERSION     DAORGN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 20220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20220
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_20220_cov_5.938557"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1044
                     /locus_tag="PKL17_10230"
     CDS             208..1044
                     /locus_tag="PKL17_10230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNK45151.1"
                     /translation="MFTRSLQSADGTALRVDGYPTELTDPKGVVVLIHGFAEHRRRYG
                     HVAKHLNQHGYHVLAGDLRGHGESGGDRGYIDRFGDYVDDVTAFIGEAQKAFPSGDTR
                     PPILVGHSMGGLVCLEYVLSHPKAVRALAITSPFFGIKIKVPGWKRSLGMAASVIHPR
                     LKLPNQIDSYILSHDKASCKAYAEDPLIFHTATARWFTEILAIHGDIRRRAPQLGIPM
                     LLLQAGDDQLVDSQAAQQVFDLLGGQDKTMKVYPGLFHEILNEVERETVLRDLTDWLG
                     TH"
     gene            1365..2564
                     /locus_tag="PKL17_10235"
     CDS             1365..2564
                     /locus_tag="PKL17_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45152.1"
                     /translation="MPLFPGNSWRDGYFVEHSGVSDFGFQVRTTGVYGRNPLGITDDE
                     VMKYAKIENRFTLHVAALVSIASWVELGLAMPFVLYQDSSGFNLSRSAVGDLRLIGKV
                     NLHLPDKSPIQAALSVGVGFQTAQIDSGLGAGQVSGYPRLILDAPGLLQKRLHIAANV
                     GAVIAGSNIPCADGSSTTTQPAMLLNPLGTLDTPGTGATADPNATPCTKLALGLGNHI
                     LYGAGISMAISQDNGLYINTELIGSFALGSTDETRAPLFWDIGLRRSKSNGTYFSVAY
                     GIGLTAGSPSHTVIAGLGLNWESKPPDKKKDGPAIKIDINLSGLPSGASAMIPSVDGP
                     GIPIGGAAPGGDKGGGDKGGGKPGGGGDKPSGGGEPKSVKASASSGSVDIPVPDGLIP
                     KEDKKAK"
     gene            2656..3489
                     /locus_tag="PKL17_10240"
     CDS             2656..3489
                     /locus_tag="PKL17_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNK45153.1"
                     /translation="MYVTKFDKADCKVDLDSISPDAPKDISRDQAELKLESLGKEMFE
                     LQDLLWGARNHAVLIVLQGLDTAGKDGAIKNVAGFLNPRGVSVVSFGVPTKEELEHDF
                     LWRVHKHTPRRGEWTLFNRSHYEDILVVRVESLCPESIWRERFGHIRDFEELLVEHNT
                     IVLKFFLHISKDEQKKRLYEREQDERTAWKLNVGDWQTREKWKDYKEAYEDALRKTAT
                     ERAPWHVVPANNKWFRNLVIAETIVETLRPYKKIWRKKLDDMGVRAKSELEAYRQRKS
                     E"
     gene            complement(3551..4528)
                     /gene="cysK"
                     /locus_tag="PKL17_10245"
     CDS             complement(3551..4528)
                     /gene="cysK"
                     /locus_tag="PKL17_10245"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNK45154.1"
                     /translation="MSSTHWFADNAQSIGRTPLVRLNRVIDSAKATVLGKIEGRNPAY
                     SVKCRLGAAMVWDAEKRGLLLPGKEIVEPTSGNTGIALAFVAASRGYPLTLTMPETMS
                     IERRKLLVAYGAKLVLTEGARGMSGAVAKAEEIAASDPSRYVLLQQFKNPANAAIHEA
                     TTGPEIWNDTAGQIDIFVAGVGTGGTITGVSRYIKQHKPIVSIAVEPAHSPVLTQKRA
                     GEPIKPGPHKIQGIGAGFVPDVLDLSVVDAIEQVTNDEAVTYARRLAREEGILAGISC
                     GAAVAVAARVARRPENEGKTIVVVLPDSGERYLSSILFEGLFDERGLAK"
     gene            complement(4626..6410)
                     /locus_tag="PKL17_10250"
     CDS             complement(4626..6410)
                     /locus_tag="PKL17_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45155.1"
                     /translation="MFTKSGYQAHINRLLHAQFSQHSETHKNKADSLLLLMQDLRQLL
                     ALEGQHVPTWTERIQQSIIPSAITPAEKLRCLSCALVFCQKLAGAFQVGSLYQIAHYQ
                     WESLDSEFDIVFPRSLSESIFALQQVSDTIDRALESTAPLAKTETLWEVSEQVVHLTR
                     AQYALRDLGDGFGPLLALVIGHFADVIKAEGEKIREDQQRAPVPNPYVIANPVQGSLF
                     VGREDVLRRVEELWGSTGQCPSVLLYGHRRMGKSSVLQNLSRLRLPEKSVLVDFNLQR
                     IGRVRSTAELLHGLSLKLYDAARKAGLSGSALAEPAETDFLDAGKNPYLALDRFLDRL
                     GTLRDGHRFLVTIDEFELIEEQIDAGRIDPQLLSVFRGVVQTYPWLVMALAGLHRLEE
                     LRHDYWNPLFGSVSAIEVSFLSEPAARTLMTAPTPDFPLDYDTKAMARIYELTHGQPY
                     LIQLICHSLVSRYNRLLSEEPVPPLRRFLLTDVEAVVAQADLFTDGLRYFQGVWDHAV
                     RSKPPGQAAVLSALAPQKDGLCLDELFPICGLSQQDLQAALRALVRHAVLVADDGRYR
                     FTVELLRRWVTGQSGQLHNRNSATEKTN"
     gene            complement(6449..8248)
                     /locus_tag="PKL17_10255"
     CDS             complement(6449..8248)
                     /locus_tag="PKL17_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK45156.1"
                     /translation="MDGIRWLHLSDLHCGQRGEKTRWPTARESFLRDLRTQCEAQGAP
                     DLIVVTGDLAFSGQKREYEKLRPILDEIDAATGTKPLWITIPGNHDLHWPAEGDATAK
                     GLRQYDEDPALRRAVHSGSDTQRYLRKLFSDYERFFKDRILSDWQARQSELSLTYSLG
                     QLPGSFLLRVQKNGIRFGLVGLNSAYLQLGGDDLEGKLCVEPEQLKDDLPRFVDGCDA
                     AILLLHHPPSWLSKRSRKLFEQDIYPPGRFVACLFGHMHGHKSETVIGSSGEPRRYLQ
                     GSSLFGLESFGQKHEERVSGYSIYELRRTRPQEGILLRKVRTAQFLDDGALRMTADPA
                     TGHAGTEYPIKLSHGEAAIPSEAQHSQPLPQSTNSNPFVHSGRITDPRLFVGRTELLR
                     RIFDDLRQGMSRALVGPAQIGKSSLLSMVCALAPLQLPQVTPVYLNLQLIDSDGDFFA
                     ALCHKLGFAESRGYKLERQLAGRRVVLCLDEIEKLRSDRFPIEVREQLRGLADGADAP
                     LTLLLASRVSLAELFPDAHAQTSPLYNICPPLSVPPFSVSECRELLAKRLQGTGVQFS
                     ETELSMLCEKSQGHPATLVADAAALFAKYRGQP"
     gene            complement(8255..8767)
                     /locus_tag="PKL17_10260"
     CDS             complement(8255..8767)
                     /locus_tag="PKL17_10260"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNK45157.1"
                     /translation="MVPLRSWAAPQLGDPAPAIDLPQLDVKKSGASLGPLLKLNPQPG
                     QVYVIDFFATWCASCLKATTALADVLPALGDRVSLIVVDVGQPPQVVADHFAAHPLPP
                     KTQVVIDPDGEAARRFGQDRFPTTFLVDDHGVIAHINRGYGPGYKARIDRWLRSMLGL
                     PPAAPKLLPQ"
     gene            complement(8855..10792)
                     /locus_tag="PKL17_10265"
     CDS             complement(8855..10792)
                     /locus_tag="PKL17_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45158.1"
                     /translation="MLNEAQLVAQAEAAAGEPVLVHTEARRQRPGEILSQRGLLAATR
                     SALVFVVDELLRPGEAKVFRTADLTGHTHALSSFAATLTLETADGRTEFSDLGRDKLT
                     PLLVQCGLQDKPDTASETETTTPALPDSLSVPFVVDFASPTFAPPTFAPEPVVSQPGR
                     LFAADFGPPKTPDTDSDSGMEHRIGDQVDKRDIDEKEDPAEEEDDADDDDADDDDDDD
                     EDDEDEDEDEVDEVDDDDDSEAKDAAIDHSEHKKSEPPPVIESKETETPKPAASQTPE
                     AVSTSIGLWILLLLIVGIGLAYWIPEQRTQHHFGITPQSVLSLMKDWRDVMSTGYVLA
                     ALVVLIGAPANALGCRPPVFFPALAAFLLLNAGLFGMEAVLPDPKHLGDTIPSAAEII
                     ERLQLANVAPLLSCVILCYLFAVPQLGLWARALATSPQHRSSSFSQTEVRAGNATPAL
                     AMGVAAIGSALFGCLYFQRASHSVAVIAISLFLISPSAILAWQGSRLDPKVKWEIGGS
                     PAALAVQGALVYFTYWLAVRQLGIVDELGFSGQSPLKIRALLGSLDSLESTLIAVDKP
                     LFSALVVAQLGIIAASMIGHDAIVTIEPGQKRSFLGWMLALSVVLFFAAMANGLYPGL
                     REQYASLFALLRNLAVGHTPG"
     gene            complement(10826..11863)
                     /gene="pdxA"
                     /locus_tag="PKL17_10270"
     CDS             complement(10826..11863)
                     /gene="pdxA"
                     /locus_tag="PKL17_10270"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNK45159.1"
                     /translation="MTHRIGLTLGDPSGIGPELWIKTLLSLRDTPDQLAQLTMFGDPQ
                     VLRFAAQNMGLESAWRTLADSIHIEAVSNLSPSEIEPGKPSQKSGISQISYLHAAIAA
                     AQRGDISGLCTGPIHKASAKAAGFAFPGHTELLAQALGDPTRSVVMMLAGPTLRVALC
                     TIHIALSQVPSALTAAGISATIKTTALALCEDFGETKPALSVVGLNPHAGEAGHFGDE
                     EARLIVPAMEQTRLDPDFVSCGATIVGPLVPDVAFRDAVHPRKGQPRPAALVAMYHDQ
                     GLIPLKLLDFDEAVNVTLGLQIVRTSPDHGTAHDIAGKGIARPDSLQAAIKMCLSHTA
                     QRRKKPRLALS"
     gene            complement(11894..12874)
                     /locus_tag="PKL17_10275"
     CDS             complement(11894..12874)
                     /locus_tag="PKL17_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002108121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK45160.1"
                     /translation="MKIFVTGASGFVGGAATRRLVAEGHTVVAMSRSEKSDAAISALG
                     ATPVRCNLDDVSAPVMAGCEVVLHCAAHVEAWGDPDVWDRINVKGTERMLQAAKQAHV
                     RRFIHIGTEAALVHGQHLRNVDEDQPLAIHSPYPYCRTKALAEQAVRRANDPDADFVT
                     IVLRPRFIWGPGDLTLLPTMLEFAAQGKWMWIDGGRALTSTTYIGNLVEGIVLALSKG
                     LPGQAYFVLDEGQRSMHDIITAMADCADVKLPSRSMPGWLAEGIGAVCEFVWRSASLK
                     GTPPLTRHAAMVMAKDCTLRGDKAAKELGYVPKFTVEQGLAGLRKTFAKS"
     gene            complement(13030..14313)
                     /locus_tag="PKL17_10280"
     CDS             complement(13030..14313)
                     /locus_tag="PKL17_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK45161.1"
                     /translation="MSKSLLTLRRGLWCSVVALCALSGSARAEIDAGVFAGVHFFNDD
                     NRLGRASHPASENGIDHGGLVGIRLSYVPLPRLAIEAELGIVPTSSHDGLSRLGVMPA
                     RAHLLVNLLTGRVRPFLLAGGGGMFAMPTAGSRLEQDAVGEFHAGGGLQVDIVNNWGL
                     RLDGRAVFPQAMSQTLTTEGEILLGFYGRFDRKEAASLPPPAPVPSPPVPVVPPPVVD
                     SDGDGILDAVDKCPQAAGLAANSGCPDSDTDGDGVIDRLDKCPAEAGVKENDGCADKD
                     SDGDGVVDRLDKCPAEPETKNQFQDDDGCPDELPKAVAQFTGAIAGIAFEPNRATITK
                     ASNPVLDKAVAVLKEHATVRIEIAGHTDNAGTPEANRTLSQQRADAVRQYFIDKGIDG
                     ARLTAVGFGPDKPIADNATPAGRAKNRRVEFRLLP"
     gene            complement(14337..15281)
                     /locus_tag="PKL17_10285"
     CDS             complement(14337..15281)
                     /locus_tag="PKL17_10285"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNK45162.1"
                     /translation="MFTKQHLFTVSALLGGLLVSAAPALAQDQVPVVKQEFGKQEEVK
                     EVIWKASASAGFSISTGNASVMTLSGGANASRNDGTNKIALGIKGIYGVTDIVVFTDA
                     NGNKVADDESELTVDRKRTAALLLGTFRYDRFFSKNNTGYLMASAGLDLPASKQFMGG
                     GQIGYSRQLVNTKMHLLSAELGYDFNYTKYIVPDVPPANFAENVFLHSARIFAGYVLS
                     VADHTSVEANVEALVNFNTAHIGDRDVGAGSATRVNGQLAFTTKVWKALSLRAATMLR
                     YNNAPGLTTAFAYDPNFAGRYNQKLDTLTELQLVVNFL"
     gene            15363..16946
                     /locus_tag="PKL17_10290"
     CDS             15363..16946
                     /locus_tag="PKL17_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK45163.1"
                     /translation="MSADRKERTRQNGGNWGIFAKIGAFLLLPSLPCALKSANLRQVR
                     SRPSLHADRGLTERGSARILTGKKDERGAARRSAVGSVLLIEDDPDQAMLVSDFLTRR
                     GLRLRSACDAEQGLLLLEEESPDLILCDINLPGISGVEFLATVRPRHPQVAMLMMTAF
                     SSIEQAIECIRLGADDYITKPLHLADLDGRIDRALESRATKTRLELLENEVRDRHRFD
                     NMIGRSAAMQRVFQIIQRAAQTSATVLISGRTGTGKELVARALHYHSNRASKPFVDIN
                     CGALPEQLVASELFGHQKGAFTGATEMRKGLFETAEGGTLFLDEVQSLKADLQTMLLR
                     ALQERTIRRVGGRENISVDVRIIAATNRDIADAVRRGEFREDLYYRLNVVSISLPDLR
                     ERTEDIPLLIEHLLKQRQGDGPPCQFSPEAMRLLQGYPWPGNVRELHNAVECALTIGR
                     PPVLQIADLPPHIALPSKGQGATERPLARTLDEVERIHILRILDELDQNHTRAAEVLG
                     IDRRTLYRKLDKYRIQHPE"
     gene            complement(17064..>20220)
                     /locus_tag="PKL17_10295"
     CDS             complement(17064..>20220)
                     /locus_tag="PKL17_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HNK45164.1"
                     /translation="WGGNLRQDVGGLGGHPQNPAPGTELFMGGGGGAGDQDNNSGGNG
                     GTGGGVVVLLSGSVTVPMGSGGLIQANGGDGADTGNSHGDAAGGGGGGGSIFLLTGKS
                     IDASVQLSANGGYGGAQKRSPSDALEADGPGGGGGGGVIVYLAGGKPTVSAKGQTGGT
                     SVASTVSKFPSNGATNGNDGISIQAPRQPTSMGMPSGYPICLPADIQVSVVPPSGMVQ
                     QGMNVDYTVTVKNAGENPALGADIVTTLPSGVDPSKVTWTCMPMGLGVTCPGGMTMGM
                     GPLPSQADLPKDGSLTFKVTLPVPQPATQPTLDLGVSAYPPPGYADPLPGNNSATGSA
                     PIAGVVVKQPVSDLEVSVKKSPTSPNPGDETTVTISAKNNGPDAAPKPVVVFSIPPGS
                     VITQPPPMPGDPLAPWSCTANGNSYTCTLKMDLPAGSSAPDLQVKFKTPTMPGTTGTP
                     QVPVLIGSPSGTDPNPGNNSAVVDVGAKQPAGSADLSLTVTKTPDTNGPTMETQFSLQ
                     AKNSGPDTAQSPYITFSIPPGSVVTQEPSGTGWACTRNGTTVTCIAPAIVSGQTASPV
                     SIKIIAPKPTAPGASPGVVAGTVDSPTTFDPNPANNVDEKPIASSTAVTPTGSDLAVK
                     ITTDKPNPMPGDEVTYTGVATNKGPDAVSNPVVVFNLPPGAVVTQPAAGDGWQCTQSG
                     TTAMCTRDTIALGSAPPIVVKVKLPTDAPSSPAGTPSTTVVVTAPKNNDPNPGNNTAV
                     VDTRPTQPATNADLAIEVSKDPASGGPGMDVTYKLSPSNNGPGSVANPSVTFTIPPGS
                     TVKQAPSGTGWTCLQNGASFTCYGSADLPVGKAAPITMVLNTPAPADPSKSAGAVSAV
                     VSSPSNSDPNPSNNQASVEVGNRTPSGSDLSIKVSRNPTGPNPSDPVVIVAEASNAGP
                     DPVSNPVITIQLPPGAEVIDEPSGDGWTCSRDASTVLCTRDSIPQGSAPPVSVTVKMP
                     SSGSGSTSGRSGSATAVISAPSNMDPNPANNIAGTDHYRLFGGGVSCSAQGHNGSQSP
                     ASLLGLALLIVLGLRRRSLSS"
BASE COUNT         3988 a         6002 c         6125 g         4105 t
ORIGIN      
        1 ggctcggtgc cgagcggcag ggtcagctcg tggatggcct cgctgctctg ctcacttgcg
       61 gcgtgtttcc gtcggtcttt gtttgccgaa cttcgcactg gatacctttt gcgtcgcgcc
      121 cagtcgggat gcgtgtgcgc tgtgtggcac gggctgaccg gttttgtcaa caagcaggcc
      181 gtgttttgct gtagtgctta tccattcatg tttacccgca gcttgcagtc cgcggatgga
      241 accgcgcttc gtgtagatgg ctacccaacc gagctgactg acccgaaagg cgtcgtcgtg
      301 ctcatccacg gctttgccga gcaccgtcgt cgctatggcc acgttgccaa acacctgaat
      361 cagcacggct atcatgtgct ggcgggtgat ctgcgcgggc atggggaatc gggcggcgac
      421 cgcggctata ttgatcggtt cggcgattat gtcgacgatg tgaccgcgtt cattggcgaa
      481 gcgcaaaaag ccttcccgtc gggtgatacg cgaccgccga tactggttgg gcacagcatg
      541 ggcggcctgg tgtgcctcga gtacgtgctg tcgcatccca aggcggtgcg cgcgctcgcg
      601 attacttcgc cgttttttgg catcaagatc aaagtgcccg gctggaaacg atcgctcggg
      661 atggcggcat cggtgattca tccgcggctc aagctgccaa accagatcga cagctacatc
      721 ctttcgcacg acaaagcgtc atgcaaagcc tacgcggaag atccgctcat cttccatacg
      781 gcgacggcgc gctggttcac cgagattttg gcgatccacg gcgacattcg gcgacgggct
      841 ccgcagcttg gcatcccgat gctgcttctt caggcgggtg acgatcagct ggtcgattca
      901 caagcggcgc agcaggtgtt cgatttgctc ggtgggcaag acaagaccat gaaggtctac
      961 cctgggctgt ttcacgagat cctcaatgaa gtcgagcgtg aaacggtgct gcgggatctc
     1021 accgattggc tggggacgca ctaagcttcc gtcgagaaac ttctgtcatg gctgcgcaga
     1081 aacagcgagt acacagcccg tgtagtcggc tgtatccgtg cagcataagc tcgcgatccg
     1141 cgagcaagaa tcggcagtag aggttttgta gttattgacc gctccacgta aaagccttta
     1201 ctttctaatg aagcggaggc agatgctaca tggcatcgtc gagtacccga gtacgatttg
     1261 atctcattgc cagtgcggcg tgtgcgctcg ccttgctggg gccagcaacg gcatacgcag
     1321 acacgacaac cacgaccacc ccgacatttg atgctcggcg agcgatgccg ctctttccgg
     1381 ggaacagctg gcgcgacggt tacttcgtcg agcacagcgg tgtcagtgac tttggcttcc
     1441 aggttcgcac cacaggcgta tacgggcgca atccgctcgg catcaccgac gatgaggtga
     1501 tgaagtatgc caagatcgaa aaccggttca cgctgcatgt ggcggccctg gtgtcgatcg
     1561 cgagctgggt cgagctgggc ctcgcgatgc cgtttgtgct gtatcaggac tcgtcgggct
     1621 ttaacctaag ccgcagcgcc gtcggagacc ttcgcctgat cggcaaagtc aacctgcacc
     1681 tgccggacaa atcgccgatt caggccgcac tgtccgtcgg tgttggtttc cagaccgcgc
     1741 agattgattc cggtttgggc gcaggacagg tcagcggtta tccgcggctt atcctcgatg
     1801 caccgggtct tttgcaaaag cggctgcaca tcgcggcgaa cgtcggcgcg gtcatcgcgg
     1861 gttcgaacat tccgtgtgcg gatggttctt cgacgacgac gcagccggcc atgctgctca
     1921 atccgctcgg cacgttggat acgccgggca ccggagcgac cgccgatccg aacgcgacgc
     1981 catgcaccaa gctggcgctc ggacttggca accacatcct gtacggcgcc ggcatttcga
     2041 tggcgatctc gcaggacaac ggtctttaca tcaacaccga gcttatcggt tcgtttgctc
     2101 tcggcagcac cgacgagacg cgagcacccc tgttttggga catcggtctg cgtcgatcca
     2161 agtcgaacgg gacgtatttt tcggtcgcct acggaatcgg tctgaccgcg ggcagcccaa
     2221 gccacacggt catcgccgga ctgggtctga actgggaaag caagccaccg gacaagaaga
     2281 aggatggtcc tgccatcaag atcgacatca acctgagtgg tctgccgtcg ggcgcgtcgg
     2341 cgatgattcc aagcgtcgat ggtccgggca ttccgattgg tggtgcggcg ccaggtggtg
     2401 acaagggcgg cggcgacaaa ggcggcggca agcccggcgg tggcggcgac aagccgagcg
     2461 gcggtggcga accgaagagc gtgaaggcca gcgcgtccag cggaagcgtc gacattccgg
     2521 ttcccgatgg cctgattccc aaggaagaca agaaggcgaa gtagcctccc cacctgctgc
     2581 tttgtagatt gcgtcttttc ggagctcggc ttgtctatct ggccggtggg ggagctccga
     2641 aaggaaccac tcccgatgta tgtaacgaag ttcgacaaag ccgactgcaa ggtcgatctc
     2701 gacagcattt cgcccgacgc acccaaagac ataagccgtg atcaagcgga gctgaagctg
     2761 gagtctcttg gcaaagagat gttcgagctt caggacctgc tgtggggggc gcgcaatcac
     2821 gccgttctga tcgtgcttca ggggctggac accgccggca aagatggtgc gatcaagaac
     2881 gtggcgggat tcctaaaccc gcgcggtgtc agcgtggtgt cgtttggcgt tcccaccaaa
     2941 gaggaactag aacacgactt cctgtggcgc gttcacaagc acacgccgcg acggggagag
     3001 tggactctgt tcaatcgttc ccactacgaa gacatcttgg tggtgcgggt ggaaagtctc
     3061 tgtccggaat cgatctggcg cgagcgcttt ggtcacatcc gcgactttga agagcttttg
     3121 gtcgagcaca acacgatcgt cctcaagttc tttttgcaca tcagcaaaga cgaacaaaaa
     3181 aagcggctct acgaaagaga gcaggacgag cggacggcgt ggaagcttaa cgtcggtgac
     3241 tggcagacgc gcgaaaagtg gaaagactac aaagaagcgt atgaagacgc gctgcgcaag
     3301 accgcgacag agcgggcgcc ctggcatgtg gtgccagcca acaacaagtg gtttcggaac
     3361 ctggtgatcg ccgaaaccat cgtcgaaacg ctgcgaccgt ataagaaaat ttggcgcaag
     3421 aaactcgatg acatgggggt gcgcgccaag agtgaactgg aagcctaccg gcagcggaaa
     3481 agcgagtagc gacgacccgg tgttggactg aatcctatat gaagcatttg ataggattca
     3541 tattggtttt ctacttggcg agtccgcgtt cgtcaaacag tccttcaaac aagatcgagc
     3601 tcaggtagcg ttcaccggaa tcgggcagca ccaccacaat ggtcttgcct tcattttccg
     3661 gacgtcgagc gacgcgcgcg gcgaccgcga cggcggctcc gcacgaaatt cccgccagga
     3721 ttccttcttc ccgcgccagc cgtctggcat atgtgaccgc ttcatcgtta gtgacctgtt
     3781 caatcgcatc gaccaccgac agatcgagca cgtcgggtac aaagccggcg ccgatgccct
     3841 gaattttgtg cgggccgggc ttgatgggtt cgccggcacg cttttgggtc aggaccggac
     3901 tgtgcgcggg ttcgactgcg atggacacaa tcggtttgtg ctgtttgatg tagcgcgaaa
     3961 ccccggtgat ggtgccaccc gtgccgacgc cagcgacgaa gatatcgatc tgaccggcgg
     4021 tgtcattcca gatttcgggg ccggtggttg cttcgtgaat cgcggcgttg gcgggatttt
     4081 tgaactgctg caggagcacg taacggctgg ggtcggacgc ggcgatttct tcggctttgg
     4141 cgacggcacc gctcatgccg cgagcgcctt cggtcagtac caatttggca ccgtaagcga
     4201 ccagcagctt gcgccgctcg atgctcatgg tttcgggcat ggtcagggtc agcggatagc
     4261 cacgcgaagc agcgacgaag gcaagggcga tgccggtgtt gccactggtg ggctcgacga
     4321 tttctttgcc gggcaacagc aggccgcgtt tttctgcgtc ccacaccatc gccgcgccaa
     4381 gacgacactt taccgagtaa gcgggattgc gaccctcgat cttgccaagt acggtggcct
     4441 tggcggagtc gatgacgcgg tttaagcgaa cgagcggtgt ccggccaatc gactgtgcgt
     4501 tgtctgcgaa ccagtgcgtg ctcgacatgg ggtttccttc ttccttccat caccgggcac
     4561 aagtgctgcg cccatggttt catccgttat aacagacagt ggatcattct aacagacggt
     4621 cgtttttagt tggttttttc cgtcgctgag ttccggttgt gaagctgtcc actttggccc
     4681 gtaacccagc ggcgcaaaag ctctaccgtg aaacgataac gaccgtcgtc cgctacgagc
     4741 accgcatggc gaacgagtgc gcgcagcgcc gcttgcagat cctgctgcga cagtccgcag
     4801 atcggaaaaa gctcgtccaa acagagtccg tccttctgcg gcgccagcgc cgagaggacc
     4861 gcggcctgac cgggcggttt gctgcgcacc gcgtgatccc agaccccctg aaagtagcgc
     4921 agcccgtcgg taaatagatc ggcctgggcc acgacggctt cgacatcggt cagcagaaag
     4981 cgacgcagcg gcggcacggg ctcttcggac agcaagcgat tatagcgcga caccaggctg
     5041 tgacagataa gctgaatcag atacggctgg ccatgggtca gctcgtaaat ccgagccatc
     5101 gcttttgtat cgtagtcgag cggaaaatcg ggcgtggggg ccgtcatcag cgtccgcgcc
     5161 gccggttcac tgaggaagct cacctcgatg gcggacaccg atccaaacag cggattccag
     5221 tagtcgtgcc gcagctcttc cagtcggtgc agtcccgcga gcgccatcac aagccacgga
     5281 tacgtctgca ccacaccgcg aaataccgac agaagctgtg gatcaatccg gcccgcatcg
     5341 atctgttctt cgatcagctc aaactcatca attgtgacca ggaatcggtg gccatcgcgc
     5401 agcgttccca atcgatccaa aaagcggtcg agcgccagat aggggttctt ccccgcgtcg
     5461 agaaaatcgg tctctgctgg ttccgcaagc gcgcttcccg aaagccccgc tttgcgcgcc
     5521 gcgtcgtaca gcttgagcga aaggccatgc agaagttccg cggtcgatcg cacccggcca
     5581 attctctgca agttgaaatc gacaagcacg ctcttttccg gcagccgcaa gcgcgacaga
     5641 ttttgcagca ccgatgactt tcccatccgc cgatgtccgt agagcagcac cgacggacac
     5701 tgcccggtcg atccccacag ctcttccacc cggcgcagca catcttcacg tccaacaaac
     5761 agcgatcctt gcaccggatt ggcgatgaca tagggattgg gaaccggcgc gcgctgctga
     5821 tcttcgcgaa ttttttcgcc ttcggctttg atcacgtcgg caaaatgacc aatcaccagc
     5881 gccaaaaggg ggccaaaacc atcgcccaag tcacgcagcg catactgcgc tcgcgtcaga
     5941 tggacaacct gttcactgac ttcccacagc gtctctgtct tggccaaagg agccgtactt
     6001 tcaagcgccc gatcgatggt gtcactgacc tgttgcagcg caaaaatcga ctcgctgagc
     6061 gacctcggaa acacaatatc aaactcagaa tcgagcgatt cccactggta gtgcgcaatc
     6121 tgatacagac tgccgacctg aaatgcgccc gccaattttt gacaaaacac aagcgcgcaa
     6181 gacagacagc gcagcttctc tgcgggcgtg atggcgcttg ggatgatgct ctgctgaatt
     6241 ctctccgtcc acgtcggtac gtgctgtcct tccagggcca gaagctgccg caagtcctgc
     6301 atcaaaagca agaggctgtc cgctttgttt ttgtgcgttt cactgtgctg cgaaaactgc
     6361 gcatgcagaa gccgattgat gtgcgcttga tatcctgact tcgtaaacat cgaccgtcgt
     6421 ccgcgtgata tggggggacg cagcggcatc acggctggcc tctgtacttg gcaaacagtg
     6481 cggcagcatc agcgacgagc gtggctgggt gtccttggct tttttcgcac agcatagaca
     6541 gctctgtttc ggaaaactgc accccggttc cttgcagtcg cttggccaga agttcacggc
     6601 attccgaaac agaaaagggc ggaacagaca gcggcggaca gatgttgtaa agcggcgaag
     6661 tctgggcgtg ggcgtcggga aacagctcag cgagcgacac ccgactggcc aacagcaaag
     6721 tcagtggggc atcggctcca tcggccagac cgcgcagctg ttcgcgcact tcgatcggga
     6781 agcggtcact gcgcagtttt tcaatttcat cgaggcacag caccacgcgg cggcctgcca
     6841 actgccgttc cagtttgtag ccgcgacttt ccgcaaaacc gagcttgtga cacagcgctg
     6901 cgaagaaatc tccatcgctg tcgatcagct gcaagttcag ataaaccggc gtgacctgcg
     6961 gaagctgcaa aggcgccagc gcacagacca tcgacaaaag cgacgacttg ccaatctgcg
     7021 ctggaccgac gagcgcacgg ctcattccct ggcgcagatc atcgaaaatg cggcgcaaca
     7081 gctcggttcg tccaacaaac agccgcggat ccgtgatccg tccgctgtgc acaaacggat
     7141 tgctgtttgt cgactgtggc agcggttggg aatgctgggc ttcagaagga atcgccgctt
     7201 cgccatgaga cagcttgata ggatactctg tcccggcatg tcccgtcgct ggatcagctg
     7261 tcatccgcag cgcgccatca tccagaaact gcgcggtgcg gacttttcgt agcaggattc
     7321 cttcctgtgg ccgcgtgcgg cgaagttcgt agatgctgta tccgctgaca cgttcttcgt
     7381 gtttctgacc gaacgactcc agcccaaaca gcgacgatcc ttgcagatat cgtcggggtt
     7441 cccctgaact gccaatcacg gtttcggact tgtgcccgtg catgtgtcca aacagacagg
     7501 cgacaaaacg ccctggcgga tagatgtcct gttcaaacag cttgcgactc cgcttgctaa
     7561 gccacgatgg cgggtgatgc agaagcagaa tcgcggcatc acagccatcg acaaagcgcg
     7621 gcagatcgtc ttttagctgt tccggttcga cgcacagctt gccttccaga tcgtcgccgc
     7681 ccagctgaag gtacgcggaa ttgagtccga ccagaccgaa acggatgccg tttttttgaa
     7741 cgcgcagaag aaaacttccg ggcagctgtc ccagcgaata cgtcagcgaa agctcgctct
     7801 gtcgcgcctg ccagtccgac aaaatgcggt ctttgaaaaa gcgttcgtag tctgaaaaca
     7861 gcttgcgcag atagcgctgg gtatccgaac cactgtgtac cgcccgtcgg agcgccggat
     7921 cttcatcgta ttgacgcagg cctttggcgg tcgcatcgcc ttccgccggc cagtgcagat
     7981 cgtgattgcc gggaatcgtg atccacagcg gctttgttcc ggtcgctgca tcgatctcat
     8041 cgaggatcgg acgcagcttt tcgtattcgc gtttctgtcc ggaaaacgcc agatcgccgg
     8101 tgacgacaat caaatcgggg gctccctgcg cttcgcactg ggtccgcaga tcgcgcaaaa
     8161 aggactctcg tgccgtcggc cagcgggtct tttcaccgcg ctgtccacag tgcagatccg
     8221 acagatggag ccagcgaatt ccgtccatgc tcacctattg tggcaaaagc ttgggcgcag
     8281 cgggtggaag accgagcatg ctgcgcagcc acctgtcgat gcgcgctttg taacccggtc
     8341 catagccgcg gtttatgtgg gcaatgacac cgtgatcgtc gacaaggaat gtggtgggaa
     8401 agcggtcttg gccgaaccga cgcgccgctt cgccatctgg atcgatcaca acctgcgtct
     8461 ttggcggcag tggatgcgcg gcaaagtgat cggcgacaac ctgcggcggc tggcccacgt
     8521 cgacgacaat cagcgaaacg cggtcgccca gcgcgggcag tacatcagcc agtgccgtcg
     8581 tcgctttcag acacgatgca caccacgttg cgaaaaagtc gatcacatag acctgacccg
     8641 gctgcggatt cagcttcaaa agggggccaa gcgatgcgcc tgattttttg acatcaagct
     8701 gcggcagatc gatcgcgggg gccggatcgc caagctgtgg cgccgcccag gatcgcagcg
     8761 gaaccaatat gaggaatata aaaagcgtat tttgtaggaa acgcaacagc gcagccacca
     8821 tcgcttgtta gcatcgccgc agggacaatc gcggtcaacc cggcgtgtgg ccgaccgcca
     8881 gatttcgcag cagagcaaac agacttgcgt actgctcgcg cagccccgga tacagcccgt
     8941 ttgccatcgc tgcgaaaaac agcacgaccg aaagcgcaag catccaaccc aaaaacgatc
     9001 gcttctgtcc tggttcgatg gtgacgatgg catcgtgtcc gatcatcgac gcggcgatga
     9061 tgccaagctg ggcaaccaca agcgcggaaa agagtggttt gtccaccgcg atcagcgtcg
     9121 attcaagcga atccagggaa ccaagcagcg cccggatttt cagcggcgac tgtccggaaa
     9181 agccaagctc atcgacgatg ccaagctgtc gaactgccag ccaataggta aagtacacga
     9241 gcgcgccttg cactgcgagt gcggcaggcg atccaccaat ttcccacttc actttcggat
     9301 caagccgtga tccctgccac gccaagatgg ccgacggtga aatcaggaac agtgagatgg
     9361 ctatcaccgc gacagaatgg cttgcacgct gaaaatacag acaaccaaac agtgcagatc
     9421 cgatggcggc cacgcccatg gcaagcgctg gagtggcatt tcccgcacgc acttcagtct
     9481 gtgaaaagga actgctgcga tgctgtgggc tcgtcgccag cgcccgtgcc cacagaccca
     9541 attggggaac cgcaaacaga taacagagga tgacacagct aaggagcggc gcgacgtttg
     9601 caagctgaag ccgctcgatg atttcggctg cggacggaat ggtgtcaccc aagtgtttcg
     9661 gatcgggaag aacggcttcc attccgaaca gaccggcgtt cagcaaaagg aatgcagcca
     9721 gagcaggaaa gaacaccgga ggacgacatc ccagcgcatt ggccggggct ccgatcaaga
     9781 cgacaagcgc ggcaagtaca tatccggtcg acatcacatc gcgccagtct ttcatcagtg
     9841 acaggacgct ctgtggggtg atgccaaagt ggtgctgcgt gcgctgttcc ggaatccagt
     9901 acgcaagccc aattccgaca atcaacagca gcaggatcca cagaccaatc gacgtgctga
     9961 cggcttctgg ggtttgggat gctgccggct tgggcgtctc tgtttctttg ctttcgatga
    10021 caggaggcgg ctcgctcttt ttgtgttcgc tgtgatcgat cgctgcatct ttcgcttcgg
    10081 aatcatcgtc gtcgtcgact tcgtcgactt cgtcttcgtc ctcgtcttca tcgtcctcgt
    10141 cgtcgtcgtc gtcgtcgtcc gcgtcgtcgt cgtccgcgtc gtcctcttcc tctgctgggt
    10201 cttccttttc gtcgatgtcg cgcttgtcaa cctggtcgcc gatccgatgt tccatgccgg
    10261 aatcgctgtc tgtgtccggt gttttggggg gaccaaaatc cgcagcaaac agcctgcctg
    10321 gttggctcac aaccggttca ggagcaaacg ttggaggagc aaacgtagga gaagcaaaat
    10381 cgacaacaaa aggaaccgaa agggaatcgg gcagagccgg tgttgtggtc tctgtttctg
    10441 acgcagtatc tggtttgtct tgcagaccac actgcacgag cagcggggtt aacttgtcgc
    10501 gtcccagatc ggaaaattcc gtccgtccat ccgcagtttc caaggtcagg gtcgctgcaa
    10561 aggatgaaag tgcgtgcgtg tgtccggtca gatccgcagt ccggaatact ttcgcttcac
    10621 cgggccgcag aagctcgtcg accacaaaca ccagcgcgct gcgggtggcg gccagaagtc
    10681 cgcgctgcga caggatttcg ccggggcgct gtcggcgcgc ttcggtatgg accaacaccg
    10741 gctcgcccgc cgccgcttca gcctgggcga ccagctgcgc ttcatttagc atcttttcat
    10801 cgtacaaaaa gcgcagcgcc agggcttagg aaagcgcaag tcgtggtttt ttccgtcgct
    10861 gtgcagtgtg cgaaagacac atcttgatag ccgcctgcaa gctatctggg cgtgcgattc
    10921 cttttccggc gatgtcgtgt gcggtgccgt ggtctggcga tgtccgcaca atctggagcc
    10981 cgagcgtcac attcaccgct tcatcgaagt cgagcagctt gagcggaatc agtccctgat
    11041 cgtgatacat cgcgacaagc gccgctggtc ttggctgtcc tttgcgcggg tgaactgcgt
    11101 cgcggaacgc cacatctgga accagcggac cgacaattgt tgcaccacag gaaacaaagt
    11161 ccgggtccaa acgggtttgt tccattgcag gaacaatcag ccgggcttct tcgtcgccaa
    11221 agtgtccggc ttcccctgcg tgtggattga gtccaacaac ggacagcgcc ggttttgttt
    11281 cgccaaagtc ttcgcaaagg gccagtgcgg tggtcttgat ggtggcggaa attcccgctg
    11341 ctgtcagcgc ggaaggcacc tgcgaaagcg cgatgtgaat cgtgcacaac gcgacccgca
    11401 gcgtcggtcc agccagcatc atcaccacac tccgcgtggg atctcccagc gcttgggcca
    11461 gcagttctgt gtggccgggg aacgcaaatc ctgcggcttt ggcggatgct ttgtgaatcg
    11521 gtccggtaca cagtccggaa atgtccccgc gctgtgctgc ggcgatcgct gcatgcaaat
    11581 aagaaatctg tgaaatgcca cttttttggc tgggctttcc tggttcaatt tccgacgggg
    11641 agaggttgga aacggcttcg atgtgaatcg aatcagccag cgtccgccac gcagattcca
    11701 gccccatgtt ctgcgccgca aagcgcaaca cctggggatc gccaaacatc gtcaattggg
    11761 caagctgatc cggtgtgtcg cgcagggaca ggagtgtttt tatccacagc tctggaccaa
    11821 ttccgctggg atcgccaagt gtcaggccga ttctgtgtgt catgttctcc tgcgcacgcg
    11881 cgaaggaatg ggatcaggac ttggcaaacg tcttgcgcag ccccgcaagc ccttgctcga
    11941 cggtaaactt gggaacatag ccaagctcct tggccgcttt gtccccgcgc aacgtgcagt
    12001 ctttggccat gaccatcgca gcatggcggg taagcggagg cgttcctttg agcgaagcag
    12061 agcgccagac aaactcacag accgcgccga tgccctctgc cagccagccg ggcatgctgc
    12121 gacttggcag cttgacgtcg gcgcagtcag ccatcgcggt gatgatgtca tgcatgctgc
    12181 gctgtccctc atcgagaaca aagtacgctt gaccaggcag tcctttgctt agtgcaagga
    12241 cgattccttc gaccagattg ccgatgtacg tggtcgacgt gagcgcgcgg cccccatcga
    12301 tccacatcca ctttccctgc gcagcaaatt ccaacatggt gggaagcagc gtcagatcgc
    12361 cggggcccca aatgaagcgc ggacggagca cgatcgtcac aaagtctgca tcgggatcat
    12421 ttgcgcggcg caccgcttgt tccgcaagtg ctttggttcg gcagtacgga tacggactgt
    12481 gaatcgccag cggctggtct tcatcgacgt tccgcagatg ctgtccgtgc accagcgcgg
    12541 cctctgttcc gatgtgaatg aagcgacgga cgtgggcctg tttggcggcc tgtaacatcc
    12601 gctctgtgcc tttgacattg atgcggtccc agacatctgg atcgccccag gcttcgacgt
    12661 gcgcggcaca gtgcagcacc acttcacaac ctgccatcac gggggcgctc acatcgtcga
    12721 gattacagcg tacaggggtc gcgccgagcg cagagatcgc agcgtcactt ttttccgatc
    12781 gtgacatcgc gaccacagta tgaccttctg caaccagcct tctcgtggcc gctccgccaa
    12841 cgaaacccga tgcacctgtg acaaatatct tcatctgcag aatctcccga tgggtgcacg
    12901 ataccagcgc ccgatcggcg tgtgaagcga tgggaatgtc aggtaaggac cggcgttggg
    12961 gggcgtcgga aggagagcgc ccgatcggcg tgtgaagcga tgggaatgtc agcggggggc
    13021 ttgggcaggt tagggcagga gcctaaactc gacgcggcgg tttttggcgc ggcccgcggg
    13081 ggtggcgttg tcggcgatcg gcttgtcggg accaaaaccc actgcggtca ggcgtgctcc
    13141 gtcgatgcct ttgtcgatga agtactgacg aacggcatcg gcgcgctgct gcgacagggt
    13201 tcggttggct tcgggcgtgc cggcgttgtc ggtgtgccca gcgatttcga tacgcaccgt
    13261 cgcatgctct tttagcaccg cgactgcttt gtcgagcacc ggattcgaag ctttggtgat
    13321 cgtcgcgcgg ttcggttcaa aggcgatgcc ggcgatggcg ccggtaaact gcgcgacggc
    13381 tttcggtagc tcgtcggggc agccgtcgtc gtcttggaac tgatttttgg tttccggttc
    13441 ggcggggcac ttgtcgagtc gatcaaccac gccatcaccg tcgctgtctt tgtcggcgca
    13501 gccgtcgttt tccttcacgc ccgcttccgc cggacactta tcaagccgat cgatcacgcc
    13561 atcgccgtcg gtgtcagaat ctgggcagcc ggagtttgcc gccagaccgg cggcttgcgg
    13621 acacttgtcc accgcatcga ggattccgtc tccgtcgcta tcgaccacag gtggcggcac
    13681 gacagggact ggtggcgacg ggaccggcgc aggtggcggc agcgacgcag cttctttgcg
    13741 atcaaagcga ccgtagaagc cgagcaggat ctcaccttcg gtcgtcagcg tctgcgacat
    13801 cgcttgcggg aacaccgcgc gtccgtcgag ccgcaggccc cagttgttca cgatgtcaac
    13861 ttgcagaccg ccaccggcgt ggaattctcc gacggcgtct tgttccagcc gcgagcctgc
    13921 ggtgggcatg gcgaacatgc cgccaccacc ggcgagcaga aacggtcgca cccgacccgt
    13981 cagcaggttg acgagcagat gcgcgcgcgc cggcatcaca ccgagtcgcg acaagccatc
    14041 atggctgctc gtcggcacaa tccccagctc ggcttcgatc gccagacggg gaagcggaac
    14101 atacgacagc cgaatgccga ccagcccacc gtgatcgatg ccattttccg acgcgggatg
    14161 gctggcgcgg cccaggcggt tgtcgtcgtt aaaaaaatgc actccggcga atacgcccgc
    14221 gtcgatctcg gcccgcgccg agcccgacag tgcacacagc gctaccacgg aacaccaaag
    14281 tccgcgacga agcgtaagca aagacttgct cataatcccc tccttgctcg gtgtggctac
    14341 aggaagttca cgacgagctg aagctcggtg agtgtgtcga gcttctggtt gtagcgacca
    14401 gcgaagttcg gatcataagc aaacgccgtc gttagaccgg gcgcgttgtt gtagcgcagc
    14461 atcgtcgctg cacgcaggct cagcgctttc cagactttgg tcgtaaacgc gagctgaccg
    14521 tttacgcggg ttgcgcttcc tgctcccacg tcacgatcgc cgatgtgcgc ggtgttgaag
    14581 ttgaccagcg cttcgacgtt ggcttcgacg ctggtgtgat ccgcgaccga cagcacataa
    14641 ccggcgaaga tccgcgccga gtgcaaaaac acgttttcag caaagttggc cggcggaacg
    14701 tcgggcacga tgtacttggt gtagttgaag tcgtagccaa gttcggcgct gagcaggtgc
    14761 atcttggtgt tgaccagctg gcgcgaataa ccaatctggc cgccgcccat aaactgcttc
    14821 gatgccggca gatcaagtcc cgccgaggcc atcaggtagc cggtgttgtt cttcgaaaag
    14881 aaccggtcgt agcggaatgt accgaggagc agcgcggcgg ttcgcttgcg atcgacggtc
    14941 agctcgcttt catcatcggc gactttgttg ccgttggcgt cggtgaacac cacgatgtcg
    15001 gtgacgccgt agatgccctt gatgccgagc gcgatcttgt tggttccgtc gttgcgtgag
    15061 gcattggcgc cgccgctcag cgtcatcacg ctggcgttgc cggtgctgat cgaaaagcct
    15121 gccgaggccg atgctttcca aatgacttcc ttgacctctt cctgcttgcc aaactcttgc
    15181 ttgacgacgg gaacttggtc ttgggcgagc gctggtgccg ctgaaacgag cagcccgccc
    15241 agcagggctg agacggtaaa aagatgttgt ttcgtgaaca tagggttacg ctccttggct
    15301 ctccgcggct gcgggagctt ttgtttggcc agcaagatta gcaatccata taccactcgt
    15361 tgttgtcggc ggatcgcaag gaacgcacaa ggcaaaacgg cggcaattgg ggcatttttg
    15421 ccaaaattgg ggcatttttg ctgctgccgt ccttgccgtg tgcgctcaaa tcggcgaacc
    15481 tgcgtcaggt cagatcgcgt ccttccctgc atgcggatcg cggtctgacg gaacgtggga
    15541 gcgcacggat tttgacgggc aaaaaagacg aacgaggcgc ggcgagaagg tctgcggtgg
    15601 gcagtgttct tttgatcgag gacgatcccg atcaagcgat gctggtgagc gattttctga
    15661 cgcggcgcgg tctgcggctg cggagcgcgt gcgacgctga acaggggctc ttgcttctcg
    15721 aagaggagtc gcccgatctc atcttgtgtg acatcaacct gccagggata agcggagttg
    15781 agtttctggc gacggtgcga ccgcgccatc cgcaggttgc gatgctgatg atgacagcgt
    15841 ttagttcgat cgagcaggcc atcgagtgca tcaggctggg ggccgacgac tacatcacaa
    15901 agcctttgca cctggctgat ctcgacggac ggatcgatcg tgcgctggaa agtcgggcga
    15961 ccaaaacgcg actcgaactg ctcgaaaacg aagtgcgcga ccgccatcgc ttcgacaaca
    16021 tgattgggcg atccgcggcg atgcagcggg tgttccagat catccagcgg gcggcgcaga
    16081 ccagtgccac cgtgctgatt tcgggccgaa cgggaaccgg caaagaactg gtggcgcgcg
    16141 cgcttcacta ccacagcaat cgggcgagca agccgtttgt cgacatcaac tgcggtgcgc
    16201 tgccggaaca gctcgtggct tcggagctgt ttggacatca aaaaggcgcg tttaccggcg
    16261 ccaccgagat gcgcaaaggt ctgtttgaaa ccgcggaagg gggcacgctg tttctcgatg
    16321 aagtgcagtc gctgaaagcc gatttgcaga ccatgctgtt gcgcgcgctt caggaacgca
    16381 cgatccggcg cgtcggtggt cgcgaaaaca tcagcgtgga tgtccgcatc attgctgcga
    16441 ccaaccgaga cattgccgat gcggtgcgcc gtggagagtt tcgcgaggat ttgtactatc
    16501 ggctcaacgt cgtttcgata agccttccgg atctgcgcga gcgcaccgaa gacattccgc
    16561 tgctgattga acacctgctc aagcagcggc agggagacgg gcccccctgt cagttttcac
    16621 ctgaggcgat gcggctgctc cagggctacc cgtggccggg caatgtccgc gagctgcaca
    16681 acgcggtgga gtgtgccttg acgattggtc gcccgccggt tttgcagatc gcggatctgc
    16741 cgccgcacat tgcgcttccc agcaagggtc aaggcgcgac ggaaaggccg cttgcgcgca
    16801 cgctggatga agtcgaacgg attcacattc tgcggattct cgacgagctg gatcaaaatc
    16861 acacccgcgc tgccgaggtc ttgggaattg atcggcggac cctgtatcgc aagctcgaca
    16921 agtatcgaat ccagcatccg gaataggaat cgaaccgcgc atccggaata ggaaacgaag
    16981 caagccggaa agtcaggaat gcggaaggga agggcggcgg accccttttg cgcgtggcag
    17041 aaaaaggggc cccaaaggcg ggcttacgac gaaagcgagc gacggcgcag acccaggacg
    17101 atgagcagcg ccagacccaa cagcgaagcc ggagactgcg acccgttgtg gccttgcgca
    17161 ctgcacgaaa caccaccccc aaacaggcgg tagtgatcgg ttccggcgat gttgttggcc
    17221 ggatttgggt ccatgttgct cggcgcactg atcacggcgg tcgctgatcc gctccgaccc
    17281 gaagtgctac ccgatccgct gcttggcatt tttacggtga cgctgaccgg gggcgcgctg
    17341 ccctgcggaa tgctgtcgcg cgtacaaagc accgtgctgg catcacgaga acaagtccag
    17401 ccgtcgccgc ttggttcatc gatcacttcg gcaccgggcg gaagctggat cgtgatgacc
    17461 gggtttgaca ccggatcggg gcccgcgttg gacgcttctg cgacgatcac cactggatcg
    17521 ctcgggtttg gtccggtcgg gttgcgactg actttgatcg acagatccga accgctcggc
    17581 gtgcgatttc cgacttccac cgatgcttgg ttgttgctcg gattgggatc actgttcgac
    17641 ggagagctga ccaccgcgga caccgcaccg gcacttttgc tcgggtcggc tggcgctggc
    17701 gtgttgagca ccattgtgat cggcgccgct ttcccaaccg gcagatcggc gcttccgtag
    17761 caggtgaacg atgcgccatt ttgcagacaa gtccagccgg ttcccgacgg ggcttgcttc
    17821 accgtgctgc ctggcggaat cgtgaacgtc acactcgggt ttgcgacgct gcctggccca
    17881 ttgttgctcg gcgacagttt gtacgtaacg tccatgcctg ggccgccgct tgcgggatct
    17941 ttgctgacct cgatggccag atcggcgttt gttgcaggct gcgtcggtcg cgtatccacc
    18001 accgcggtgt tgttgccggg gtttggatcg ttattcttcg gcgccgtcac caccaccgtc
    18061 gtcgatggcg tcccagctgg cgagctgggg gcatcggtcg gcagcttgac tttgaccaca
    18121 atcggcgggg ccgatccgag cgcaatcgtg tcgcgggtac acatcgcggt ggttccgctc
    18181 tgcgtgcact gccagccatc gcccgccgcc ggctgcgtca ccaccgcacc tggcggcagg
    18241 ttgaacacga cgaccggatt gctgaccgca tcggggcctt tgttggttgc gaccccggtg
    18301 tacgtcactt catcgcccgg cattggattc ggtttgtcgg tcgtgatctt gaccgcgaga
    18361 tccgatcccg tgggcgtgac tgccgtgctg ctggcaatcg gtttttcatc gacgttgttg
    18421 gccggattcg gatcaaacgt cgtcggagaa tccaccgttc cggccaccac gcccggcgat
    18481 gcgcccggtg ccgtcggttt gggcgcgatg attttgatcg aaaccggact cgcggtctga
    18541 cccgacacaa tggccggggc gatgcatgtc accgtcgtgc cgttgcgcgt acacgcccag
    18601 cctgttcccg acggctcttg cgtcaccaca ctgccaggag gaatcgaaaa cgtgatgtac
    18661 ggactctgcg cggtgtccgg tccactgttc ttcgcttgca gcgaaaactg ggtttccatc
    18721 gtcgggccgt tggtgtccgg cgtcttggtc acggtcagcg acaaatccgc cgatcctgcc
    18781 ggctgcttgg caccgacatc aaccaccgcg gagttgttgc ccggattggg atcggttccg
    18841 ctcggcgagc caatcagcac cggcacttgc ggcgttcccg tcgtgcccgg catcgtcggt
    18901 gtcttgaact tcacctgcag atccggcgcg ctgcttcccg ccggcagatc cattttcagc
    18961 gtgcaggtat agctgtttcc gttcgcggtg cacgaccacg gcgcaagcgg atcgcccggc
    19021 atcggcggtg gctgcgtgat cacacttccg ggcggaatcg aaaacacgac cactggtttt
    19081 ggcgccgcat ccggtccgtt gtttttggcc gaaatcgtga cggtggtttc atcccccgga
    19141 ttgggcgacg tcgggctctt tttgacactg acttccagat ccgaaacggg ctgcttgacc
    19201 accacaccag ctatcggcgc cgatccggtt gcgctgttgt ttcccggcag tgggtcggca
    19261 tagcccggcg gcggatacgc gctgactccc agatcgagcg tcggttgcgt cgcgggctgc
    19321 ggcaccggca gcgttacttt gaacgtcagc gatccatctt tgggcaagtc cgcttgcgac
    19381 ggcagcgggc ccatgcccat cgtcatgccg cccggacacg tcacgccaag acccatcggc
    19441 atgcaggtcc aggtgacttt gcttgggtcg actcccgaag gcagcgtcgt cacgatgtcc
    19501 gcacccagcg ccggattttc acccgcgttt ttcaccgtca cggtgtagtc gacattcatt
    19561 ccctgctgca ccatgcccga cggcggcacg accgatacct gaatatcggc gggcaagcag
    19621 atcgggtatc cgctgggcat tcccatcgaa gtcggctggc gcggcgcttg gatcgaaatg
    19681 ccgtcgttgc cattggttgc gccattcgat ggaaacttgc tgaccgtcga tgcaacgctg
    19741 gttccgcccg tctgtccctt ggccgatacc gttggtttgc cgccggccaa atagacgatg
    19801 acgccgccgc cgccaccgcc gcccggaccg tcggcttcga gtgcatcact cggcgatcgt
    19861 ttctgcgccc caccatatcc accgtttgcg gacagctgga cgctcgcgtc aatcgacttc
    19921 ccggtgagca ggaagatcga tccaccgcca ccgccgccgc ccgccgcatc accgtggctg
    19981 ttgccggtat ctgctccgtc gccgccgttg gcttgaatga gtccgccgct gcccatcggt
    20041 accgtcacac tccccgatag gagcacgacc acgccgccgc ccgtcccgcc attgccaccg
    20101 gagttgttgt cctgatcacc ggcgccaccg ccaccgccca tgaacagctc cgtaccgggg
    20161 gcgggatttt ggggatgacc accgagccca ccgacatctt ggcgcaggtt gccgccccat
//
LOCUS       DAORGN010000076        20213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1046_length_20213_cov_5.788670, whole genome shotgun sequence.
ACCESSION   DAORGN010000076 DAORGN010000000
VERSION     DAORGN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1046_length_20213_cov_5.788670"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKL17_10300"
     CDS             <1..1154
                     /locus_tag="PKL17_10300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45165.1"
                     /translation="ALSAAHEKNVYHRDLKPDNIMLVPDHDSEVPGRERVKLLDFGIA
                     KVAQSDEPGSPHSAQPKTSTDVVMGTPRYMAPEQCKGGVQIDDKADVYSLGVMLYEMV
                     AGRPPFVGGSGEVLAMHIYEPPQPLKQLAPHCPEEIAQLVHLLLSKKREERPSMKQVV
                     VRLEELGQRHPTSMLPAISMAALLPPSTTPPGSNPLQTPAAGISGASTTGLSAGQATP
                     AGRSPKMLLLGLLGGGTALLGVLAFLLVRPQPQQPVVIAPVKTVTPDLAMPPPPPPKP
                     KQIHWSLISTPPGAQVVRVSDGQILGGTPMEMDLPIGDGTCDVKLRLPGFAEKVLKLS
                     LAESESRKETLDKAPPARVGNKRWGVGGNKTTSNPNPTKGNDDQPRIVD"
     gene            1189..1878
                     /locus_tag="PKL17_10305"
     CDS             1189..1878
                     /locus_tag="PKL17_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45166.1"
                     /translation="MPQKAAAQADAGSLAQSPAPKKKDDGCLRDNVCRTAYQKALALY
                     DEGRYEEALTGFQSAYSRRQMPWLLLNIGRTVQRLGRPKEAISYYERYKKADPNIDPE
                     TERRVNKYIEQAQALLEQTPAQADMPRPDPVVQTGNTNGTPINPNLTPQPQPPLNLTP
                     PQPTPTPAETQPVATPIYKKWWFWTIIGGVVAAGVVGTAVGVTASAKKDPLPAQPEPF
                     QPGDTVLNPQF"
     gene            1891..4047
                     /locus_tag="PKL17_10310"
     CDS             1891..4047
                     /locus_tag="PKL17_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45167.1"
                     /translation="MSKSELRPVLSPGSRKGSRLSLAALPLAMVTCGDPGQLEPDANA
                     VAVTVIGLTADATKLVVTPTLDGKAATTTTPFEVTSKLTHFGLRMAKELSGTLTIAID
                     AYDVELCKVGTGSVNTPVGKPWRYEVSASMKTVSPRQCPPPPPPKTCAPNLFCWSNPL
                     PQGNTIQALWALSGSDVWAVGDAGSVFHYDGTKWSSSSSGTTETLYGVWAASASEVVA
                     VGTNGKVIRWNGTNWTAEASGTAKRLTAIWGNSTDAWAVGEGGTIIKRSGGTWSSQTS
                     GVTSNLNGVWGRSSSEVYAVGDTGVIRKFDGSNWTAMTSTTTMNLSSMVGDATSAVAV
                     GQNGTIVSLSGSTWSAQTSGTTEQLSAVFSPASGQFVSVGTNGTILRGSAGTWTSQPA
                     GTSLALTAVRGTGSSDIWSAGVGGQLMRYDGTKWTSSRSGFERNIRSVAALKTTDVWA
                     VGDGGYIGHYDGTKWTDFPSGTTVNLNGVYALSATEVYAVGDNKTFLRYLGSSWQPHP
                     AGYATIAQDVKGIWAFAGTNIWTVATTTTPNQVYVGNFNGISYSGYTPTDPAGMNRVV
                     NVNGMWATQQGANTLIFFAGKSYAAFSNVNGTMVNTSYQFPLNGSELRAVWGTSATDV
                     WVVGDNGFVIQYDGAWKSQTTGLAPANLYGVWKSAPTSELWMVGDLGMVWKYDGTAWA
                     VKESTTRNKLQAITGISTTDFWVGGSSGTMLHTQPQ"
     gene            complement(4056..4772)
                     /locus_tag="PKL17_10315"
     CDS             complement(4056..4772)
                     /locus_tag="PKL17_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK45168.1"
                     /translation="MTDTPKRKSTSPAATAGFAKRRKNDASSAATKPTEPVAGDPLPH
                     EPRVGDNLRILRLERGMSLAELAERSEVSKAMLNQIEMGKSSPTIALGWKIANGLGVP
                     FGALLGEPQPGDFVVHRSDQMQVLYSDGKALRSTALFPAGDSRVAEMYELQLEPGKEE
                     LASAHSPGTREQIYVTAGQLVVEAAGNQATLQVGDVLFFCADRPHRYHNPGREPVRCI
                     LLMLYPKDRKPTPEPGMVKL"
     gene            complement(4815..6047)
                     /gene="clpX"
                     /locus_tag="PKL17_10320"
     CDS             complement(4815..6047)
                     /gene="clpX"
                     /locus_tag="PKL17_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNK45169.1"
                     /translation="MHGLSCSFCGKSQKEVRKLIAGPTVYICDECIGLCNDIIAEEIE
                     KDDQPFGQSSVPKPSEIKNVLDEFVIGQDRAKKILSVAVHNHYKRIDAKVGPEEVELQ
                     KSNILLLGPTGCGKTLLAQTLARMLNVPFAIADATNLTEAGYVGEDVENIIVSLLNNA
                     DHDVERAQRGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKILEGTVCNVPPKGG
                     RKHPQQEFLQVDTTNILFICGGAFVGLEDVIEQRVGQKNMGFGANIQSKHEKKIWDFL
                     KEVQPEDLLKYGLIPEFIGRLPVIAPLHELTEDALVDILTKPRNALIKQYQKLFEMDG
                     VKLRFTKGALKAISQAALKTRSGARGLRAIVENILLETMYEIPSHPNIKEVLVSEEVV
                     EQNQQPLRIYHQKDAASA"
     gene            complement(6107..6712)
                     /gene="clpP"
                     /locus_tag="PKL17_10325"
     CDS             complement(6107..6712)
                     /gene="clpP"
                     /locus_tag="PKL17_10325"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNK45170.1"
                     /translation="MSYIIPTVVEQTHRGERGWDIYSRLLKDRIVFLGTPVDDMVANT
                     IIAQLLFLESEDPDKDVMLYINSPGGLVTAGMAIYDTMQLIRCDVATICMGQASSMGS
                     FILSGGAKGKRYALPHSRILIHQPLGGFSGQATDIEIHAREILRTREKLIELYSHHTG
                     QPEERIRRDMERDFFMSAAEAKEYGLVDEVLYKKKSEAKAP"
     gene            complement(6875..9235)
                     /gene="clpA"
                     /locus_tag="PKL17_10330"
     CDS             complement(6875..9235)
                     /gene="clpA"
                     /locus_tag="PKL17_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNK45171.1"
                     /translation="MITKELQESLLSAMAEARKRRHEYLCLEHVLYSFTEEPTSAKIL
                     RACAVDLAKLRKQLEEFLTTKVASLPDGVDHEPEQTVSIQRVLQRAIFHMHSSGKSQI
                     SSGDVLAAMFREQDSHAIFLLESQGVTRLDVVNYLSHGIEKDRPGDDESEPNELQLSR
                     PEPSHDGESEDEDGGESDKKRSLLEKFTVNLVAEAKAGRVDPLIGRENEVQRTMQVLC
                     RRRKNNPLYVGDAGVGKTALAEGLAWKIARGEVPEVLKDSQVFALDMGGLVAGTKFRG
                     EFEQRLKGILSELRKIKKAILFIDEIHTIVGAGAVSGGAMDAANLLKPALASGELRCI
                     GSTTFEEYKTVFDKDRALSRRFQKIDLVEPSADETFLILKGLRARYEEFHGVRYTDPA
                     LRAAADLAAKHMTDRHLPDKAIDVIDEAGAAMRILPGGVGKSKKVVRPADIEQVIAKM
                     AKIPPKSVSADDKTQLQNLDAELKKVIYGQDSAIDSLVTSIKLSRSGLGNPEKPVGCF
                     LFSGPTGVGKTELAKQLARVLGVEFIRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGLL
                     TDAVRKTPHAVLVLDEIEKAHPDIYNVLLQVMDHATLTDNNGRKADFRNIVLVMTTNA
                     GAREIQQGTIGFGAAGPAKNLDKSAIERTFSPEFRNRLDAWITFQSLSPEIIRRVVDK
                     FTSQLFDQLKQKKVMIELSDEARSWLAEKGYDRLNGARPMARLIQNELKRPLADAILF
                     GELQNGGVVRVSVSDGKLVLQIEPRDGGSKDSSSKEPASKSSASAK"
     gene            9376..10341
                     /locus_tag="PKL17_10335"
     CDS             9376..10341
                     /locus_tag="PKL17_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45172.1"
                     /translation="MQVCPQCTHELSTASDLELGAPFACPHCGVTLPLTAVHSELYDL
                     PVPLRPSSPHLRMNEESLPVPLREAPDYRSERPRSSPPRPLPLALLPTETEGEPRLRQ
                     LPPPGVPTFSVGPISNPPATAAQITLPEQAAAKITAPSQPAILASDIRIDHEARSLAE
                     RSTTQAKPIVLEHEPPPSVEPQGSEAKPAEPTTKPIVVDPPIVSASQPVATSVDPGPL
                     AKLSPRAGSTQSAKPISLAARSPTPSSQNSAGSQPGDMPLSTMFSMAASRQSLTSQTP
                     QALKLGWLIAGLALAALLLLLTILVLVDRGPTGNQPALPTTGTMP"
     gene            complement(10378..11655)
                     /locus_tag="PKL17_10340"
     CDS             complement(10378..11655)
                     /locus_tag="PKL17_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNK45173.1"
                     /translation="MTRYVSPADALSHVRSGMRVFIQTAAAAPQVLISAMIDRASELR
                     GVEIVHMHTEGSAAYVEPQYRDSFHASALFVGHNLRDAVNAGRADYIPVFLSEVPRLF
                     RRGILPLDVALLHVSPPDRHGFCSLGVSVDVARAASDCARILIAQVNPNMPRTHGDGL
                     IHISRLTAMVECADPLIECVPKPLSNVERAIGRQVAGLVEDGATLQMGIGAIPDAVLA
                     ALGSHRRLGIHTEMFSDGVIDLVERGIITNESKKVHPGAIVAGFAIGSRRLYDFMDDN
                     PMVKMLDIAYVNDTDVIRRNPQVTAINSALEVDLTGQVCADTLGGKQYSGVGGQMDFI
                     RGAALSENGRPIIALPSVTSRGESRIVPMLKPGAGVTTTRAHVHYVVTEYGVAMLYGS
                     SLRQRAQSLIDIAHPDHRDALSKQAHERFGLLI"
     gene            complement(11744..12736)
                     /gene="tal"
                     /locus_tag="PKL17_10345"
     CDS             complement(11744..12736)
                     /gene="tal"
                     /locus_tag="PKL17_10345"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNK45174.1"
                     /translation="MNASQDAQPSLLTQLKTMTVVVADTGDFHGIARFTPRDATTNPS
                     LVQAAAQQPDYADVVEGALRLTRHELSDRPLAEQAGAALDQLAVEFGLRILDLIPGRV
                     STEVDARLSFDTAATIAKARQIIAKYESRGQRRERILIKIAATWEGIRAASVLEREGI
                     HCNLTLLFGLHQAIACAEAGVTLISPFVGRILDWYLKDTGRTSYPSAEDPGVRSVTDI
                     YNYYKHFGYRTEVMGASFRNMGEICELAGCDLLTISPKLLAELESTRTQLVRKLDSGA
                     ARAMRVEKIAMDERTFRAMHTQNRMADDKLSQGIADFAKAQVAVEDMLAKRLSE"
     gene            12816..15065
                     /locus_tag="PKL17_10350"
     CDS             12816..15065
                     /locus_tag="PKL17_10350"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK45175.1"
                     /translation="MPGAFRYIDHDGDKRATFLLPDGCAIGRRPSKHLICLSDDSVSS
                     GHAWIEFRDDGWWVSDLGSATGTFVNDRRIMSSALRSGDILRCGNFPLRFEATPPAKG
                     ALLAGLVFVSLDGQRQTVMIHPEGGALGTFSGATVIGEHDSIARIHAFVYYDGEQWMI
                     VPVARGYETKINGHTISASGTRLRAGDAIQCGALQVRFVLLRDKLPALSSDQRRAQPN
                     NCPPESSPLSVRSASPLTAGVSSMLAAPSGFSVTPRARLSYRDDKQLPIVVEVPASGG
                     FLGRALECLVRSGDLTVPRKWGRIFFRDNRWLFEDLGSSSPSFVNDEQQFPHVPRELR
                     SGDTLRNRSMVLVFSSDGLLAQGSTIGFVKVDPPPPPPVSGSYFLLLSDDGESKQIPI
                     PDTGAVLGRSRGCLLMTEDPLTSRQHARIIWEQDIAWLEDLSKDQGTWHGGERLLGER
                     RRLHTGDLIRAGQIRARFVGKDASFPQLDERFRPFRIRCPIGNIGPLRLYWADHPELS
                     ATVILRVLPADAPDHARWLSTLVAESRLLRIHAASSPLGGTLLREKPDGERFLSVPLG
                     CGLLLSEFLSTYGPPPLPLLLELLGQLCADIEQRSQRDPTRQDGQLPLREVWLYADPI
                     APLGMATRLLPLAGTTGQRPSPVLQAPESSLTPPNVQRHSQVYVYGLLLYALASGSLP
                     SHATSQDKLLGDLRPQLPPVLFDLLHELLHVPADKRPDPYHAQRWIAELRSLLCVHGV
                     TEGNAATIC"
     gene            15105..16064
                     /locus_tag="PKL17_10355"
     CDS             15105..16064
                     /locus_tag="PKL17_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK45176.1"
                     /translation="MKTARRIGMALTLFFLGCETTGTAGPAMHVDTPAMVDPQKTLQI
                     SMVDVGQGDGMVLKTPSGVVIAFDGGPDRTGNYADFLKRQNITQIDYVILSHGHSDHY
                     TGLGTAIAMLPKDCAPRVFDPGFARPDIPGYQYIRDTAGCRYSSLTLNQTLMLDPAIE
                     LSVMGVSNTPYPQNDGTGINNTSAVIRLRYGAFSMIFQGDAQTEAEKNLYSMFKTQLA
                     ANILKVGHHGSCNATGTSLLQAIAPSYALISSGGPNDPNMFGHPHCQTIDKLRSAGVH
                     YYRTDTNGDITITTDGRVFAIVPTKGLPDSAACPRACATPSDF"
     gene            16158..16313
                     /locus_tag="PKL17_10360"
     CDS             16158..16313
                     /locus_tag="PKL17_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45177.1"
                     /translation="MSNTTSTELRRKRNHKKAGQRRKKVESKRSTLTPTELFAGFGEP
                     GKPAPKK"
     gene            16357..16833
                     /locus_tag="PKL17_10365"
     CDS             16357..16833
                     /locus_tag="PKL17_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNK45178.1"
                     /translation="MSVEIPPTTFVIGIGNLLRRDDGVGLFVAQRLRDRVPAGVEVRA
                     CAADLCDLALWLCRCQRALLVDAASGLAPGEVVRLDVFRTPRPRREATSSHALPLSQA
                     VALAEALGPLPTTLGLYAIGASDFGFGEGLSPLVAAAAETTIRQILAELSSPPHPL"
     assembly_gap    17106..17205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17206..17411
                     /locus_tag="PKL17_10370"
     CDS             <17206..17411
                     /locus_tag="PKL17_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45179.1"
                     /translation="FAQHRAWQPSFPRSFAQHRLSSLASHSVWRDRISVAALSAPFCS
                     VFSVVAGLSAAVLAKQHQAGTEN"
     gene            complement(17498..18016)
                     /locus_tag="PKL17_10375"
     CDS             complement(17498..18016)
                     /locus_tag="PKL17_10375"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNK45180.1"
                     /translation="MKIVIMGNSGSGKSTLARYVATECGLPLLDLDTVAWQPGKVAVP
                     RDPVLAQQDVDSFCQRSAGWVVEGCYADLVAVTLPYDPLLLFLDPGTEVCLRNCQSRP
                     WESHKYASPLAQNEMLPLLLAWVAAYETREGPLSLRGHVALYERYVGRKQRLREQRPA
                     SEILRGMLAFGG"
     gene            18194..19738
                     /locus_tag="PKL17_10380"
     CDS             18194..19738
                     /locus_tag="PKL17_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45181.1"
                     /translation="MPTSDQPLPREKIGSYRLIRKIGHGGMGEVYEARHEGVGGRAAI
                     KILRLDVASSPSHIERFFDEARAANSIEHPSIIRIFDSGQTNDGVCYLAMEFLDGETL
                     AKRIKTLGKLSSASAIRFARQIASALAAVHSRGVIHRDLKPDNLMIVKDPESAGGERI
                     KVLDFGIARLAADLRTRDGQTESGMILGTPMYMSPEQCHGAKQVTPPSDVYSLGIILY
                     KMLAGRAPFVSQGHGALIAMHLSDIPAPVEKYNPQVPPLLSSLIAEMLAKSPEDRPTM
                     EAIEQRLRAMSNIEDLPAPQSRSETATRKLSTVDLEQTQHLHQASLLPPQATVPLQTL
                     VVSPANPANEPASVQITSPQGRTRRLIRVLLVGCTMVGVAFCFLLFRCANEQPSSASQ
                     QVNVSPRLLAIPDLARPEKSPAAASTQPDGQPTPLVLLDAGVPLPEIRRANPVKPDPS
                     SRAVSAPAEAPPVSSRKDLRVEDEESVAAEPVSQPADLAPPIPSPVNRPTGFRSSDGL
                     LNPFPR"
     gene            complement(19764..>20213)
                     /locus_tag="PKL17_10385"
     CDS             complement(19764..>20213)
                     /locus_tag="PKL17_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45182.1"
                     /translation="SVKGGKVVASITADAPLWIDLSRLQVYVNGSLVKEIPITNRSHP
                     ALKTDIQLDISSDGWIVVLALGDVPLPTDVIGAVNGGAALPIGFTNPIWLDADGDGKV
                     TPSNAVPVRPLPWKQAALRAGFDRASLFETLHAPLDCEPFEYPDWLK"
BASE COUNT         3914 a         6450 c         5850 g         3899 t
ORIGIN      
        1 cggcactgag cgcagcgcac gaaaagaacg tctatcaccg cgacctgaag ccagacaaca
       61 tcatgctggt gcccgaccac gactccgagg tgccgggtcg cgagcgggtc aagctgctcg
      121 acttcggtat cgcgaaagtg gcgcagagcg acgagccggg ttcaccgcac tcggcgcagc
      181 caaagacgtc gacagacgtt gtgatgggca cgccgcgtta catggcgccc gagcagtgca
      241 aaggcggcgt ccaaatcgat gacaaagccg acgtgtattc gcttggcgtg atgctgtacg
      301 aaatggttgc ggggcgcccg cctttcgtcg gtggctcagg cgaagtgctg gcgatgcaca
      361 tttatgaacc gccgcagccg ctcaagcagc tggcgccgca ctgtccggaa gaaattgccc
      421 agcttgtgca tctgctgctg tcgaaaaaac gcgaagagcg gccttcgatg aaacaagtgg
      481 tggtccggct ggaagagctg ggccagcggc acccgacgtc gatgctgcca gcgatttcga
      541 tggcggcgct tttgcctccg tcgacaacac cgccagggag caatccgctt cagaccccag
      601 cagcaggaat ttcgggcgca tcgacgacgg gactgtccgc aggacaagcg accccggcgg
      661 gacgctcgcc caagatgctg cttctcggct tgctcggtgg gggaacggcg ctgcttggtg
      721 tgctggcgtt tcttttggtt cgtccgcagc cgcagcagcc ggtggtgatt gcgccagtca
      781 agacggtgac gcccgatctg gcgatgccgc caccgccgcc accgaagccg aagcagattc
      841 actggtcgct catctcgacg ccgcctgggg cccaggttgt gcgggtcagc gacggtcaga
      901 tcctgggtgg aacgcccatg gaaatggatc tgccgattgg cgatggaacc tgcgacgtga
      961 agctgcgact gccgggtttt gccgaaaaag tgctcaagct cagcctcgcg gaaagcgaga
     1021 gccgcaaaga gacgctcgac aaggccccgc cagctcgcgt cgggaataag cgctggggcg
     1081 tcggtggcaa caagacgacg tcgaatccga atccgacgaa gggcaatgat gatcaacctc
     1141 gcatcgtgga ctagacgact agcgctcggg gccgtgctca tcgcgacgat gccacaaaag
     1201 gcagcggcgc aagccgacgc aggttctctt gcgcagagcc cggctccgaa gaaaaaagac
     1261 gatggctgtc tgcgcgacaa cgtctgccgc accgcgtatc aaaaagcgct cgcgctctac
     1321 gacgaaggtc gctacgaaga agccctgacc ggtttccaga gcgcatactc ccgtcggcaa
     1381 atgccgtggc tcctgctcaa catcggtcgc acggtgcagc ggcttggccg gcccaaggaa
     1441 gcgatttcgt actacgagcg ctacaaaaaa gccgacccga acatcgaccc cgagacggag
     1501 cgtcgcgtca acaaatacat cgagcaggcc caagcgctgc ttgagcagac gccggcgcag
     1561 gcggacatgc cgcggcccga tccggttgtg cagacgggga acacgaacgg cacgccgatc
     1621 aatcccaacc tgacgccaca gccgcagccg ccgctcaacc tgacgccgcc ccagcccacg
     1681 ccgacgcctg cggagacgca gcccgtcgcg acgcccattt ataaaaagtg gtggttctgg
     1741 acgatcattg gtggcgtcgt cgctgccggt gtggtgggca ctgcggtcgg cgtcactgct
     1801 tcggcgaaaa aagatccgct accggcccag ccggaaccat tccaacccgg cgacaccgtc
     1861 ctcaatccac aattttgaga gaagcctaag atgagcaagt ccgaactccg acctgtcttg
     1921 tcccccggtt cccgcaaagg gtcgcggctt tcgctggctg cgctaccact cgctatggtg
     1981 acctgcggcg atccgggtca gctggagccg gacgcaaacg ccgtcgcagt gaccgtgatt
     2041 ggtctgactg ccgatgcgac caagctcgtc gtgacgccaa cactcgacgg aaaagccgcg
     2101 acaaccacga cgccatttga agtcacgagc aagctcaccc attttggcct gcggatggcc
     2161 aaagagctga gcggcacgct gaccatcgcc attgatgcct acgacgtcga gctgtgcaaa
     2221 gtcggcaccg gcagcgtcaa cacgccggtg ggcaagcctt ggcgctatga agtcagtgcg
     2281 tcgatgaaaa cggtgagccc ccgtcagtgc ccgccaccgc cgccaccgaa gacctgcgcg
     2341 cccaacctgt tttgctggtc gaacccactg ccgcaaggca acaccatcca agcgctgtgg
     2401 gcactatcgg gaagcgatgt ctgggccgtc ggtgacgccg gaagcgtctt tcactacgac
     2461 ggcaccaagt ggtcttcgtc gagcagcggc acaactgaaa cgctatatgg cgtctgggca
     2521 gcatcggcca gcgaagtggt cgctgtcggc acaaacggca aggtcattcg ctggaatggc
     2581 acaaactgga ccgcggaagc cagcggcacc gccaagcgcc tgacggccat ttggggcaac
     2641 agcaccgatg catgggccgt cggagaaggc ggcaccatca tcaagcgctc gggcggcaca
     2701 tggtcgtcgc agacgtccgg cgtcacaagc aacctaaacg gcgtctgggg ccgatcgtcg
     2761 agcgaggtct acgccgttgg tgacacgggt gtgattcgca agttcgacgg cagcaactgg
     2821 accgcgatga ccagcaccac gacgatgaat ctgtcgtcga tggttggcga tgcaacgagc
     2881 gcggtcgcag tcggtcaaaa tggcaccatc gtgtcgctgt cgggcagcac ttggtcggcg
     2941 caaacgtccg gaaccaccga gcagctctcc gccgtttttt caccagcgag tggtcagttt
     3001 gtttcggtcg gaacaaacgg aaccatcctg cgtggcagtg ctggcacctg gacctcgcag
     3061 ccggcaggaa ccagcctcgc gctgaccgcc gtgcgcggca cgggtagcag cgacatctgg
     3121 tccgccgggg tcggtggaca gcttatgcgc tacgacggca ccaagtggac atcgtcgcgc
     3181 tccggctttg aacgcaacat tcgcagcgtc gcggcgctca agaccaccga cgtctgggcg
     3241 gtcggggacg gcggctacat cggtcactac gacggcacca agtggaccga cttcccaagc
     3301 ggtacgacgg tcaacctaaa cggcgtgtat gcactgtccg caaccgaagt ctacgccgtc
     3361 ggagacaaca aaacgttcct ccgctacctc ggatcaagct ggcagccgca cccggctggc
     3421 tacgccacga tagcgcagga cgtgaagggc atctgggcat tcgcgggaac caacatctgg
     3481 accgtcgcga ccaccacgac cccaaaccag gtctacgtcg gcaacttcaa cgggatcagc
     3541 tattcgggtt acacgccaac tgatccagcc ggaatgaatc gcgtcgtcaa cgtcaacggg
     3601 atgtgggcaa cccagcaggg tgccaataca ctgattttct tcgccggcaa atcctacgca
     3661 gcgttttcaa acgtcaacgg cacgatggtc aacacaagct atcagttccc gctcaatggc
     3721 agcgagcttc gtgcggtgtg gggcacgtca gcaaccgatg tctgggtggt gggggacaac
     3781 ggtttcgtga tccagtacga cggtgcatgg aagtcgcaaa ccacgggcct tgctcctgcc
     3841 aatctgtacg gcgtttggaa atcagccccg accagcgagc tctggatggt cggcgacctc
     3901 ggtatggtgt ggaaatacga tggaacggcg tgggcagtga aagaaagcac gacccgcaac
     3961 aagctccagg cgatcaccgg catcagcacc accgacttct gggtcggcgg atcaagcggc
     4021 acgatgcttc acacccagcc gcagtagcgc aaacgctaca gcttcaccat gcccggttcg
     4081 ggcgtcggct tgcgatcctt gggatacagc atcagcaaga tgcagcgcac aggctcgcgt
     4141 ccggggttgt gatagcgatg cggacggtca gcgcagaaaa acagcacgtc cccgacttgg
     4201 agtgtcgctt gattgcccgc agcttcgacg acaagttgtc ccgcggtgac gtaaatctgt
     4261 tcgcgcgtgc ccggactgtg tgccgatgcc agctcttctt ttcccggctc tagctgcagc
     4321 tcgtacattt cggccacacg cgaatcacca gcgggaaaca gcgcggtcga gcgcagggct
     4381 tttccatcgg aatacagcac ctgcatctga tcggaccgat gcaccacaaa gtcacccggc
     4441 tgcggctcac cgagcagcgc cccaaacggc acgccgagtc cgttcgcaat cttccagccc
     4501 agcgcaatcg tcggtgagct tttgcccatc tcgatctgat tgagcatcgc tttgctcact
     4561 tcgctccgct cggccagctc cgccaacgac atcccacgtt ccagccgcag gattcgcagg
     4621 ttgtcaccga cgcgcggctc atgcggaagc ggatcgcccg ccacaggttc cgtcggcttt
     4681 gtcgccgccg agctcgcgtc gtttttgcga cgcttggcaa aaccagcggt ggccgcagga
     4741 gacgtcgact tgcgcttggg cgtgtctgtc atggcaaaag ggcaggcaaa gaccgcggcg
     4801 tcgaaaaaat cgacctaggc cgacgcggcg tctttttgat ggtagatgcg cagcggctgc
     4861 tggttctgct cgacgacttc ttcgctgacc agcacttctt tgatgttggg atgcgacgga
     4921 atttcgtaca tcgtctcaag caggatgttt tcgacgatgg cccgcagtcc acgcgccccg
     4981 ctccgcgtct tgagcgccgc ctgcgaaatg gccttgagcg cgcctttggt aaagcggagc
     5041 ttgacgccgt ccatctcgaa cagcttctgg tactgcttga tgagcgcgtt gcgcggcttc
     5101 gtcagaatgt cgacgagcgc atcttcggtc agctcatgca aaggcgcaat caccggcaga
     5161 cggccaatga actcgggaat cagcccgtac ttgagcaagt cctctggctg aacttccttg
     5221 agaaagtccc agattttctt ttcgtgcttg ctctggatgt tggcgccaaa gcccatgttc
     5281 ttttggccga cgcgctgctc gatgacgtct tccaaaccga cgaacgcacc gccgcagatg
     5341 aacaggatgt tcgtggtgtc gacttgcaaa aactcttgct gcggatgctt tcgaccgccc
     5401 ttcggtggca cgttgcagac ggtgccttcc aggatcttga gcagcgcctg ctgaacgcct
     5461 tcaccggaca catcgcgggt aatcgacggg ttgtcgctct tgcgcgcaat cttgtcgatt
     5521 tcgtcgatgt acacgatgcc acgctgcgcc cgctcgacgt catggtcggc attgtttagc
     5581 aagctgacga tgatgttttc gacatcctca ccgacgtacc cggcttcggt caggttcgtc
     5641 gcatcggcaa tggcaaacgg cacgttgagc atccgcgcca gcgtctgcgc gagcagggtc
     5701 ttgccgcagc ccgtcggccc aagcagcaag atgttcgact tctgaagctc gacttcctcg
     5761 ggaccgactt tggcgtcgat gcgcttgtag tggttgtgaa ccgcgaccga cagaatcttt
     5821 ttggcgcggt cctgaccaat cacaaactcg tcgaggacgt tcttgatctc cgacggtttg
     5881 ggaacgctgc tctgcccaaa cggctgatcg tctttctcga tctcctcggc gatgatgtcg
     5941 ttacacagac cgatgcactc atcgcagatg tacaccgttg ggcccgcaat cagcttgcgg
     6001 acctcttttt gactcttccc gcagaacgag caagacagac cgtgcatacg tcccctccgt
     6061 cacgaaagct cgacggtgct agtgcctact tcagattgtg ccggtgttac ggcgctttcg
     6121 cttccgactt cttcttgtag agcacttcgt cgaccagacc gtactctttg gcctcggctg
     6181 cgctcatgaa aaagtcgcgt tccatgtcgc ggcgaatccg ctcttccggc tggccggtgt
     6241 gatgggagta cagctcgatc agcttttcgc gggtgcgcag gatttcacgc gcgtgaatct
     6301 cgatgtccgt cgcctgaccc gaaaaaccac ccagcggctg gtgaatcagg atgcgcgagt
     6361 gtggcagtgc atagcgcttg cccttggctc cgccggacaa aatgaacgaa cccatcgacg
     6421 acgcttggcc catgcagatc gtcgccacgt cgcagcgaat cagctgcatc gtgtcgtaaa
     6481 tcgccatgcc cgccgtcacc agaccgcccg gcgagttgat gtagagcatc acgtccttgt
     6541 cgggatcttc cgattcaaga aacagaagct gcgcgatgat cgtgttggcg accatgtcat
     6601 cgaccggcgt gcccaaaaac acgatgcgat ccttgagcag tcgcgagtaa atgtcccagc
     6661 cgcgctcacc acggtgggtc tgctcaacga cggtcggaat gatgtacgac atggcgggct
     6721 gcgaaagcgc gcccgaagtc ttgcggtctt gcatggtcat tcgatcctct ggggttgcgt
     6781 ccggattggg aagcttggcg cagattcttt agctcaacat caccttgggt caacgtcaag
     6841 acttgccttg cgattccgtg tacttggcca cggcttattt ggcgctggcg gaacttttgg
     6901 atgccggttc tttgctgctg ctgtctttgc tgccaccgtc gcgcggttcg atctggagca
     6961 cgagctttcc gtcggaaacc gagacacgca cgacgccgcc attttgcagc tcgccaaaca
     7021 aaatcgcgtc ggccagcggc ctcttgagtt cgttttggat aagccgcgcc atcggacgcg
     7081 cgccattgag ccggtcgtag cccttttcgg caagccagct ccgcgcttcg tcggaaagtt
     7141 caatcatcac tttcttctgc ttgagctggt caaacagctg cgacgtgaat ttgtcgacga
     7201 cgcgacggat gatctcggga cttagcgact ggaacgtgat ccaagcatcg agccggttgc
     7261 gaaactctgg gctgaacgtc cgctcgatcg ccgacttgtc gaggtttttc gccggacccg
     7321 ccgcgccaaa accaatcgtg ccttgctgaa tttcccgcgc gcccgcgttg gtcgtcatca
     7381 ccagcacgat gttgcggaag tcggccttgc gcccgttgtt atcggtcagc gtcgcgtggt
     7441 ccatcacctg gagcagcacg ttgtaaatgt cgggatgggc tttttcgatc tcatcgagca
     7501 ccagcaccgc gtgtggcgtc ttgcgaaccg cgtcggtgag cagcccgcct tgatcaaaac
     7561 caacatagcc cggcggcgcg ccgataagcc gcgacaccgt gtgcttttcc atgtattcgc
     7621 tcatgtcgaa gcgaatgaac tcgacgccga gcacccgcgc cagctgcttg gccagctcgg
     7681 ttttgcccac acccgtcggg cccgaaaaca ggaaacaacc caccggcttt tccggattgc
     7741 cgagccccga gcgcgacagc ttgatcgacg tgaccagcga atcaatcgcc gagtcctgcc
     7801 cgtagatgac tttctttagc tcagcatcca ggttctgaag ctgcgtcttg tcatcggcgc
     7861 tgaccgactt gggcggaatt ttcgccattt tggcgatgac ttgctcaatg tccgccggac
     7921 gcaccacttt tttgctcttg ccgacgccac ccggcaaaat ccgcatcgcc gcgccggctt
     7981 cgtcgatgac gtcgatcgct ttgtcgggca agtgacgatc cgtcatgtgc ttggcggcca
     8041 gatccgccgc cgcccgcagc gccggatcgg tatagcggac gccgtggaat tcttcgtagc
     8101 gtgcccgcag acccttcagg atcaggaacg tttcatctgc cgaaggctcg acgagatcga
     8161 ttttctggaa acgccgcgac agcgcccgat ctttgtcgaa caccgtcttg tattcctcga
     8221 acgtcgtcga tccaatgcag cgcagctccc ccgacgcgag cgccggtttg agcaggttgg
     8281 ccgcatccat cgcgccgccc gatactgcgc ccgccccgac gatggtgtga atctcgtcga
     8341 taaacaggat cgcttttttg atcttgcgca gctcgctgag gatgcctttt aaccgctgtt
     8401 caaattcccc gcggaacttg gttccagcga cgagcccgcc catgtcgagc gcaaacacct
     8461 gcgagtcttt tagcacttcg ggcacttcgc cgcgcgcgat cttccacgcc agtccctcgg
     8521 cgagcgcggt cttgccgaca cccgcatcac cgacgtagag cgggttgttt ttgcgccgac
     8581 gacacagcac ctgcatcgtg cgctgcactt cgttttcccg cccaatcagt ggatcgacgc
     8641 gaccggcttt ggcttccgcg acgaggttga cggtgaattt ttccagcagc gaacgctttt
     8701 tgtcgctctc gccgccgtcc tcgtcttccg attccccgtc gtgcgagggc tccggtcgtg
     8761 aaagctgcag ctcgtttggt tcgctttcgt cgtcgcccgg acggtctttt tcgattccgt
     8821 gcgacaagta gttcacgaca tccagccgcg tcaccccttg gctttccagc aagaagatcg
     8881 cgtggctgtc ctgttcgcga aacatcgccg ccagcacgtc acccgagctg atctgcgatt
     8941 ttcccgacga atgcatgtgg aaaatcgccc gctgtagaac gcgctggatg ctgacggtct
     9001 gttcgggttc gtgatcgaca ccgtctggca gcgatgcgac cttggtcgtc agaaattcct
     9061 ccagttgctt gcgcagcttg gccagatcga cggcacaggc gcgcaggatt ttggcggatg
     9121 tcggctcctc ggtaaacgag tacaggacgt gttccagaca cagatactcg tgacgacgct
     9181 tgcgggcttc ggccatcgcc gaaagaagcg actcttgtag ctctttggta atcatgggct
     9241 gccgcgatgt aaaaacgaac ataacgctgc cccacccggc ccgccatccc ccgccataaa
     9301 gttcctcgca aactgcatca ctttggacaa gtcagacgac aaaacttgcg gatacactag
     9361 cgagatagac ctaggatgca agtctgtccg cagtgcacgc atgagctaag caccgcaagt
     9421 gacctcgaac tgggggcgcc tttcgcctgt ccgcactgtg gggtcacgct gccgcttacc
     9481 gcggtgcatt ccgagctata cgatctgccg gtcccgctgc gtccatcgag cccgcacctg
     9541 cgaatgaacg aagagtcgct gccggtgccg ctgcgggaag cccccgatta tcgtagcgag
     9601 cgaccccgat cgagtccgcc acgcccactt ccgcttgcgc tcttgccgac ggaaaccgaa
     9661 ggggagccgc gactgcgcca gctacccccg ccgggtgtgc cgacgttcag cgtcgggccg
     9721 atcagcaatc caccagcgac cgcggcacag atcacactgc ccgaacaggc cgccgcgaaa
     9781 atcacagccc caagtcagcc cgcgattttg gcgtccgaca ttcggatcga tcacgaagcg
     9841 cgctcgcttg ccgaacggtc aacgacccaa gccaagccga ttgtgctcga gcatgagcca
     9901 ccgccatctg tcgagccgca gggaagcgaa gccaagcccg ccgaaccaac cacaaagccc
     9961 atcgtcgttg atccaccgat cgtttctgcc agccagcccg tcgcgaccag cgtggacccc
    10021 ggcccgctcg ccaagctgtc cccgcgcgcc ggcagcaccc aaagcgccaa gccgatttcg
    10081 cttgccgcgc gcagtcccac accgtcttcg caaaacagcg cgggcagtca gccgggcgac
    10141 atgccgcttt cgacgatgtt ttcgatggct gcgtcgcgtc agagcctgac ctcgcagaca
    10201 ccacaagcgc tcaagctggg ctggctgatt gcgggtctgg cgctcgcggc gctgttgctc
    10261 ttgctgacga ttttggtatt ggtcgatcgg ggtccgacgg gaaaccagcc agcgctgcca
    10321 accaccggca cgatgccgta accaccacag gccacggcgg cggcgttttt ttaccgatta
    10381 gatcagcagg ccaaagcgct cgtgcgcttg tttcgagagc gcatcgcggt gatccggatg
    10441 cgcgatgtcg atcaagctct gggcgcgctg ccgcaggctc gacccgtaca gcatcgcgac
    10501 gccgtattcg gtgacgacat agtgaacgtg ggcccgcgtc gtcgtgacac ccgcgccggg
    10561 ttttagcatc ggcacaatcc gcgattcacc gcggcttgtc accgatggaa gcgcaatgat
    10621 cggacgaccg ttttccgaca gtgcggcgcc acggatgaag tccatctggc caccgacgcc
    10681 cgaatactgc ttgccaccaa gcgtgtcggc gcagacctgg cccgtcagat cgacttcaag
    10741 cgcgctgttg atggccgtca cctgcggatt gcgacgaatc acgtcggtgt cgttcacata
    10801 cgcgatgtcg agcatcttca ccatcgggtt gtcgtccata aagtcgtaca gccgtcggct
    10861 gccgatggca aaaccggcga cgatcgcacc cggatgaact tttttcgatt cgttggtgat
    10921 gatgccgcgc tcgaccagat cgatgacacc atcggaaaac atctcggtgt ggatgccgag
    10981 ccggcgatgc gaaccgagcg ccgccagcac cgcgtccgga atcgcgccaa ttcccatttg
    11041 cagcgtcgcg ccgtcttcca ccagacccgc aacctgccga ccgatggcgc gctcgacgtt
    11101 ggacagtggc tttggcacac actcaatcag cggatcggca cactccacca tcgccgtcag
    11161 tcgactgatg tggatcagcc catcgccgtg cgtgcgcggc atgttgggat tgacctgggc
    11221 aatcaggatg cgcgcacagt cgctcgcggc gcgggccaca tcgaccgaaa ccccgagcga
    11281 gcagaagccg tgccgatccg gcggcgagac gtgcaaaagc gccacatcga gcggcaaaat
    11341 tccgcgacgg aacagccgcg gcacttcgga cagaaacacc gggatgtagt cggcccgtcc
    11401 agcgttcacc gcgtcgcgca ggttgtgacc gacaaacagc gcgctggcgt gaaagctatc
    11461 gcgatactgc ggctcgacgt aagctgccga accttcggtg tgcatgtgga caatttcgac
    11521 gccacgcagc tccgaggcac ggtcgatcat cgcggaaatg agaacctgcg gggccgccgc
    11581 cgcagtttgg atgaacacgc gcatgcccga gcgcacatgg ctcaaagcat ccgcggggga
    11641 aacataccga gtcatggggc cccattctgc ttagatgcgg agcaaaagca atggcgagag
    11701 cccgtgcaaa aagactgggg cctgggccgg tcggatcgcc tgattattcg gaaagtcgct
    11761 tggcgagcat gtcttcgacg gcaacttggg ctttggcaaa gtcggcgatg ccttggctca
    11821 gcttgtcgtc cgccatccgg ttttgggtgt gcatggcgcg gaaggtgcgt tcatccatcg
    11881 cgattttttc gacgcgcatg gcccgtgccg caccgctgtc cagcttgcgc acgagctggg
    11941 tccgcgtcga ttccagctcg gccaatagtt tcggcgagat cgtcagcaga tcgcagccgg
    12001 ccagctcgca aatttcgccc atgttgcgga agcttgcccc catcacttcg gtgcggtagc
    12061 caaagtgttt gtagtagttg tagatgtcgg tgacgctgcg gacgccggga tcttcggcgg
    12121 acggatacga agtccgtccc gtgtctttga gataccagtc caggatgcgg cccacaaacg
    12181 gcgaaatcag cgtcacacct gcctcggcgc acgcaatcgc ctggtgcagt ccaaacagca
    12241 gcgtcaggtt gcagtgaatc ccttcgcgtt ccaacacgga cgccgcccgg attccttccc
    12301 acgttgcggc gatcttgatc aggatgcgct cgcgacgctg accgcgcgat tcgtacttgg
    12361 cgatgatctg acgggctttg gcgatcgtgg ccgccgtatc aaacgacagt cgggcatcca
    12421 cctccgtcga aacccggccc ggaatcagat ccaaaatgcg cagaccaaac tccacggcca
    12481 gctgatcgag cgccgccccc gcctgctcgg ccaaaggccg atccgaaagc tcgtgccggg
    12541 tcagccgaag tgcgccctcg accacatcgg cataatcggg ctgctgggcg gcggcttgca
    12601 ccaggctcgg gttggtcgtc gcgtcgcgtg gcgtaaagcg agcgatgcca tggaaatccc
    12661 cggtgtcagc gacgacaacg gtcattgtct taagctgcgt aagcaggctg ggctgcgcgt
    12721 cttggcttgc gttcatgggg gggactttag caaaacccga caagcaactg cgcagcgtcg
    12781 cttttctgcg tcggctcagg atagaattgg cgtcggtgcc gggagcattt cgttatatcg
    12841 accacgacgg cgacaagcgg gccacgttcc tgcttcccga cggttgcgcc attggccgac
    12901 gacccagcaa acacctgatc tgcctgtccg atgacagcgt cagctccggg cacgcctgga
    12961 tcgaatttcg cgacgacggc tggtgggttt ccgatctggg ctcggccacc ggcacgtttg
    13021 taaacgaccg ccggatcatg agcagtgcgc tgcgcagcgg cgacatcctc cgctgcggca
    13081 acttcccgct ccgctttgaa gccaccccac cggccaaagg cgcgctcctt gcgggcctgg
    13141 tgtttgtgtc gctcgatgga cagcggcaga ccgtgatgat ccatcccgaa ggcggcgcgc
    13201 tcggcacgtt ttctggtgcc accgtcatcg gcgaacacga cagcatcgcg cgcatccacg
    13261 cctttgtgta ttacgacggt gagcagtgga tgatcgtgcc ggtcgcgcgc ggctacgaga
    13321 ccaagataaa tggtcacacc atatcggcga gcggcacgcg gctacgggcc ggcgacgcca
    13381 tccagtgcgg agcgctgcaa gttcgttttg tccttttgcg cgacaagctg ccggccctaa
    13441 gctccgacca acgacgagcg caacccaaca actgtccgcc ggaaagttcg cccttgtcgg
    13501 ttcgatcggc atccccactg accgctgggg tttcgtcgat gctggcggcg ccgtcgggat
    13561 tttcggtgac gccgcgggct cggctttcgt atcgcgatga caaacagctc cccatcgtcg
    13621 tcgaagtgcc cgcaagcggc ggctttttgg gtcgcgccct ggaatgtctg gtccgatcgg
    13681 gcgatctgac ggtgccccgc aagtggggtc gcattttttt tcgcgacaac cgctggctgt
    13741 ttgaagacct gggcagctcc agtccatcgt ttgtgaacga cgagcagcag ttcccacacg
    13801 tcccccgcga gctgcgatcg ggcgacacgc tgcgcaaccg atcgatggtg ctggtctttt
    13861 cgtccgacgg gcttttggcc caaggcagca cgattggctt tgtcaaagtc gatccgccgc
    13921 ccccgccgcc ggtttccggc tcctactttc ttttgctgag cgacgacggc gaaagcaagc
    13981 agatcccgat tccggacacg ggtgcggtgc tcggacgatc ccgcggctgt ctacttatga
    14041 ccgaagatcc gctgaccagt cggcagcacg cgcggatcat ctgggaacaa gacatcgcgt
    14101 ggctcgaaga cctgtccaaa gaccaaggca cctggcacgg tggcgagaga cttttggggg
    14161 aacggcggcg ccttcacacc ggcgacttga ttcgcgccgg gcaaatccgc gcccgcttcg
    14221 tcggcaaaga tgccagcttc ccgcagctcg acgagcgctt tcgaccgttc cgcattcgct
    14281 gtccgattgg gaacatcggg ccactgcggc tctactgggc cgatcatccc gagctttcag
    14341 cgacggtgat tttgcgtgtc ctgccagccg atgcgcccga tcatgcgcgc tggctgtcta
    14401 cccttgtcgc agagtcgcgg cttttgcgaa ttcatgcggc gtccagtccg ctcgggggga
    14461 cgcttctgcg cgaaaaaccc gacggcgagc gctttttgtc ggtgccactc ggctgcggtc
    14521 ttttgctcag cgagtttttg tcaacctacg gcccgccacc actgccgctg ctgctggaac
    14581 ttttgggcca gctgtgtgcc gacatcgaac agcgcagcca gcgcgatccg acgcggcaag
    14641 acggccaact gccgctgcgc gaagtctggc tctatgccga tccgatcgcg ccgctcggga
    14701 tggcgacgcg gctgttgccg ctggcgggaa caactgggca gcgtccgtcg ccggttttgc
    14761 aagcgcccga gtccagcttg acgcccccca atgtgcaacg ccacagccag gtctacgtgt
    14821 acgggctcct tctatacgcg ctggcatcgg ggagtctgcc atcgcacgcg acctcgcaag
    14881 acaagctgct tggggatctg cgtccgcagc tgccgccggt cctgttcgat cttctgcatg
    14941 aacttttgca cgtcccggcg gacaagcgac ccgatccgta tcacgcccag cgctggattg
    15001 cggagctgcg cagcctgctg tgtgtccacg gtgtaaccga gggaaacgcc gcgacgattt
    15061 gttgagatgg tcacaagccg cgcttccgtt gcatgctgcg agttatgaag acagcgcgcc
    15121 ggatcggaat ggctctgacc ctctttttcc tcggctgtga gacgacgggg accgctggac
    15181 ctgcgatgca cgtcgacacg cccgcgatgg tcgacccgca aaagacgcta cagatttcca
    15241 tggtcgatgt cggtcagggc gatggcatgg tcctgaagac cccatccggc gtcgtgatcg
    15301 cattcgacgg tggacccgat cgcaccggca actacgctga cttcctgaaa agacagaaca
    15361 tcacccagat cgactacgtg atcctgtcgc acggtcactc cgatcactac accggcttgg
    15421 gaacggcgat tgcgatgctg cccaaagact gtgcgccgcg cgtcttcgat cctggctttg
    15481 cccgccccga catccctggt taccagtaca tccgcgacac cgccggctgt cggtacagca
    15541 gtctgacgct caaccaaacg ctgatgctcg acccggcgat cgaactttcg gtgatgggcg
    15601 tttcgaatac gccgtatccg cagaacgacg gaaccggaat caacaacacc tcggcggtga
    15661 ttcggctccg ctatggcgca ttttcgatga tctttcaggg cgacgcgcag accgaagcgg
    15721 aaaagaacct ctactcgatg ttcaaaacgc agctggccgc aaacatcctg aaggtcggtc
    15781 accacggcag ctgcaacgcc accggcacat cgctgctgca agcgatcgcg cccagctatg
    15841 cgctgataag ttccggtggt ccaaacgatc ccaacatgtt cggccacccg cactgccaga
    15901 ccatcgacaa gctgcgcagc gccggcgttc actattaccg caccgacaca aacggcgaca
    15961 tcacgatcac caccgacggc agagtcttcg cgattgtgcc aaccaaagga ctgcccgatt
    16021 ccgccgcctg cccgagagcc tgcgccacgc ccagcgactt ctaacccctt gacaagacac
    16081 gactcctaca ttcagactgc ggcccttacg gcggttttcc cgccttaccg cactctattt
    16141 caaccgggag tactccgatg tcgaatacca ccagcaccga gctgcgccgc aagcggaacc
    16201 acaagaaagc cggccagcgt cgcaagaagg tcgaaagcaa gcgctcgacc ctgaccccca
    16261 ccgagctgtt cgcaggcttc ggcgagccgg gcaagcccgc tcccaaaaag taagctgcgc
    16321 agcggtttca cttccgcctt ccgtcccgcc tctccgttgt ctgtcgaaat tccccccact
    16381 acgttcgtaa ttggcattgg caatctgctg cgccgtgacg atggcgtcgg tctttttgtc
    16441 gcccaacgtc tgcgcgaccg cgtccccgct ggggtcgaag tccgtgcgtg cgccgccgat
    16501 ctttgcgacc ttgcactgtg gctttgccgc tgccagcgcg cactgctcgt ggatgcggcg
    16561 tcaggccttg cccccggcga ggtcgttcgt ctggatgttt tccgcacgcc gcggcctcgg
    16621 cgcgaggcca catcctcaca tgctctgcca ctgtcccaag ctgtggcgct agccgaggcg
    16681 ctcggtccgc tgccgaccac actcggtctg tacgcgatcg gagccagcga cttcggtttt
    16741 ggcgaagggc tcagtccttt ggtcgctgcc gcggcagaaa cgaccatccg ccagatcctc
    16801 gccgagcttt catcgccacc ccacccgctg taatccccgg ccaacattcc gctttgttgg
    16861 ctgggaagcg ctgcccgcac cgttttgttc tgttttttcg gatggggaag ctcggccatt
    16921 cccacttgtt gcgcaaaact tgcgtggcag tccagcttcc cgcgctcttt tgcgcaacat
    16981 cgtgcgtggc agcccagctt cccgcgctct tttgcgcaac atcgtgcatg gcagcccagc
    17041 ttcccgcgct cttttgcgca acatcgtgca tggcagccca gcttcccgcg ctcttttgcg
    17101 caacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctttt gcgcaacatc
    17221 gtgcatggca gcccagcttc ccgcgctctt ttgcgcaaca ccggctgagt agcttggctt
    17281 cccactccgt ttggcgcgac agaatcagcg tggccgcgct gtccgcaccg ttttgttctg
    17341 ttttttcggt tgtggccggt ctgtccgcag cagttctggc gaagcagcac caggcaggca
    17401 cggagaactg atcgagatgt tcccgcttgc gcaagcaaag cccgttgacc agcagctgat
    17461 gaacgccgcg ggggccgtgc gaccgctgtc cgtaacgcta cccgccgaat gccaacatcc
    17521 cacgcaagat ttctgatgct ggccgctgtt cgcgcagccg ctgtttgcgt cctacatacc
    17581 gttcatacag cgcaacatgt ccgcgcagag acagtggtcc ttcgcgcgtt tcataagcgg
    17641 ccacccacgc aagcagaagc ggcagcattt cattttgtgc cagaggcgag gcgtacttgt
    17701 gcgattccca tggccgactt tggcagttgc gcaggcagac ttcggtgccc ggatccaaaa
    17761 acaaaagcag cggatcatag ggaagcgtca ccgcgaccag atcggcgtaa cagccttcga
    17821 ccacccagcc cgcgctgcgc tggcaaaagg aatccacatc ctgctgcgcc aaaaccggat
    17881 cgcgcggcac agcgactttc cccggctgcc aagccaccgt atccaaatcg agcagcggca
    17941 gcccacactc cgttgccaca taccgcgcca gcgtagactt ccctgacccc gaattcccca
    18001 tgatcacaat tttcattccc acaccttttc acggaaaaac gctgcgcacc tgcgaattta
    18061 caccgccgtc ctgtggttgc cacacgatgt gggcaagagc agaggacaca cggtctccga
    18121 tctcctcctt gggttccgtg aagcagacat tgcggacaga tggaccttgg cgatacacta
    18181 cctgcgtagt gtcatgccca catccgacca gccgcttcct cgggagaaaa ttggcagcta
    18241 ccgactgatc cggaaaatcg gacacggcgg aatgggcgaa gtctatgaag cccgtcacga
    18301 aggtgttggc ggtcgcgccg cgatcaaaat cctgcggctg gatgtcgcat caagtccttc
    18361 tcacatcgag cgtttctttg atgaagcgcg cgccgccaat tcgatcgaac acccgagcat
    18421 catccgcatt ttcgattccg gacagacaaa tgacggcgtc tgctatctcg cgatggaatt
    18481 cctcgatggc gagacgctgg caaagcggat caaaacgcta ggcaaactct cctctgcttc
    18541 cgccatccgc ttcgcacgac agatcgcatc ggcgctcgcc gccgtacaca gccgtggcgt
    18601 gatccaccga gacctgaagc ctgacaacct gatgattgtc aaagatccgg aatcggcggg
    18661 aggagagcgc atcaaagtgc tcgacttcgg aatcgctcgg ttggctgcgg atctgcgcac
    18721 gcgggatgga cagacagaga gcggaatgat tctgggaacg ccgatgtaca tgtccccgga
    18781 acagtgtcat ggcgccaaac aagtcacgcc cccatcggat gtgtactcgc tcgggatcat
    18841 cttgtacaaa atgctggcgg gacgcgctcc gtttgtaagt caaggacacg gcgcgctcat
    18901 tgcgatgcac ctgagcgaca ttccggcgcc ggtcgaaaag tacaatccgc aggttccgcc
    18961 gctgctgtct tcgctgatcg cagaaatgct cgccaagtca ccggaagatc ggcccaccat
    19021 ggaagccatc gagcagcggc tgcgggcgat gagcaacatc gaagaccttc cggcaccgca
    19081 gagccgcagc gaaaccgcga cccgcaagtt atcaacggtc gatctggaac aaacccagca
    19141 tcttcaccag gcgagcctgc tgccaccgca agccaccgtt ccgctccaaa cgctggtggt
    19201 ttccccggca aatccagcca atgagccagc cagtgttcaa atcacaagtc cacagggacg
    19261 cacccgccgc ttgatccgcg tccttttggt cggctgtacg atggtcggcg tggcgttttg
    19321 ttttctgctg tttcgttgtg caaacgaaca accatcgagc gccagccagc aagtcaacgt
    19381 ctcgccacgt ctgctcgcga ttcccgattt ggcgcgaccg gaaaaaagcc ctgccgcagc
    19441 gtcgactcag ccagacggac aaccgacccc tctggttctt ttggatgccg gcgttccgct
    19501 tccggaaatc cgccgcgcaa atcctgtcaa acccgacccg agcagtcgtg ccgtctctgc
    19561 cccagcagag gcaccgcccg tttcttcccg caaagacctg cgtgtggaag acgaagagtc
    19621 cgtcgcagct gagcctgtca gccagcctgc ggatctcgcc ccaccgatcc catcgccagt
    19681 caatcgaccc acaggattcc gatcaagcga cggcctgctc aatccctttc cacgctagcg
    19741 gcgcacaggt gactccggca aacctacttg agccaatcgg gatattcgaa gggttcgcag
    19801 tcgagggggg cgtggagggt ttcaaaaagg gaggcgcgat cgaaaccggc tcgcagcgcg
    19861 gcttgtttcc acggcagcgg acgaaccgga actgcattgg aaggagtcac tttcccatcg
    19921 ccatcggcat cgagccagat cggattggta aagcctatcg gcagcgcggc cccaccattt
    19981 accgcgccaa tcacatcggt gggaagcggt acgtccccca gcgccaaaac gacgatccat
    20041 ccgtcgctgc tgatgtccag ttggatgtct gtctttagcg ccggatggct gcggtttgtg
    20101 atcggaattt ctttgacgag cgatccattt acgtacacct gaagccgcga caaatcgatc
    20161 cacagcggtg catctgcggt gatgctggcg acgacttttc cgcctttcac aga
//
LOCUS       DAORGN010000077        20156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1052_length_20156_cov_5.959156, whole genome shotgun sequence.
ACCESSION   DAORGN010000077 DAORGN010000000
VERSION     DAORGN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 20156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20156
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_20156_cov_5.959156"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /gene="alaS"
                     /locus_tag="PKL17_10390"
     CDS             complement(<1..1898)
                     /gene="alaS"
                     /locus_tag="PKL17_10390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNK45183.1"
                     /translation="MSSQIGASAQASTRVRSLFLDYFRRQGHEVVRSSPLVPSDPTLL
                     FTNAGMVQFKDVFTGQKQLDYKRASTSQKCVRAGGKHNDLDNVGRTARHHTFFEMLGN
                     FSFGDYFKTDAILFAWEFLTKELALDPKRMCVSVFAGDSGLKLGPDDEARELWRKLSG
                     YGDDRIFGLGMKDNFWMMGDTGPQGPCSEIHYWLGDGAADLSRFGDEPGPDGSGWVEI
                     WNLVFMQFERAQKDAPLVPLPRPSIDTGAGLERVTAVAIGQRSNYDTDLFIPLLETVA
                     AACGKPYRRTDSDDDVGMRVLADHSRATANLIADGVLPSNLSRGYVLRSIMRRAIRFA
                     VRLGLPAGFFASLCLEVTKLLGDIYPELRKAESLIEKAVNAEDEGFRSTIDRGLRLIS
                     ETEFAADTAGQKTLPGKVAFQLHDTYGFPLDLTQVIGREQGFVVDEYGFAEEMRKQRE
                     RSKFAGSGDQAVSHAYHALRAAHGGTTFLGYAHGRDGICAEGRILALIVGGQSVDAAA
                     AGQEVEVLTDRTPFYGRSGGQAGDTGRIVKIVGSETVAEVEVTDTWKPVPELIVQKAR
                     VVSGSLFVGDEVSLLVDGERRDRTRCNHSATHLLHHALREVLGQHVTQKGSYVGPDKL
                     TFDFSHFSP"
     gene            2056..3591
                     /locus_tag="PKL17_10395"
     CDS             2056..3591
                     /locus_tag="PKL17_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HNK45184.1"
                     /translation="MSAHAAVTLPNGQSVPVGTALQGYLNGSANNNNINEMINAQQDA
                     QVEPQKFSPLCDFSGRYIAKGGGANFAIGWYNVDDARASNNPPKYVPVDNGANLNTAA
                     PTSDIQILFPFSANLPPANMLDLTAASIRSNAKYKGGLIGFVLVPNPNGTGAGATQYH
                     YTEHRFNVYCSKCTTPGPWYSTLIYKSNQLANTFYLGFEDLDFIDATGNTGVNGNDLD
                     YEDFLFRFTGVVCLGAGQPCTDSTQQGACQIGVTECDAQGKIVCKAIVKPNQNKDICD
                     GVDNDCNGLVDDNATCPVGQICSKGGCSAPCGSEVLCPVGQQCQMGRCIDDACVGKTC
                     AANQVCRGGSCVSPCSGIKCPSPYICSGGSCIDPCVGVTCGQGKTCSNGACITTCDCL
                     PCSSGQACLSSTGKCVDSGCEKITCSAGQACVKGSCVDACAGAVCPTGQMCTAGKCID
                     IPPDGGGNVDAGTDPTPIDGGSISTGCTCRFAPAQNGSSAWGILAGLGLLAVSARRRR
                     RRS"
     gene            complement(3588..4298)
                     /locus_tag="PKL17_10400"
     CDS             complement(3588..4298)
                     /locus_tag="PKL17_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45185.1"
                     /translation="MPTSRYVSLLGSALLACISLSACDSSKNDGELGRATFVVRNCGG
                     VLSDLQGCPLSAKLASSGKIDLIATAKSDNSLLSVRTDLPAVLKIEEPTARSYILTGM
                     HVGEAKLIAYNDSGDVDRISVFVEEVQQLAYNEISSAAGTFKLQPSGDIDGTLVLRND
                     LNSFNLYFAQLDGSQQKMLGREAFSFTSDPGLTFQAGQDKPSTLEFHYLRPKAGTYNL
                     LINSKAGPGRFKLQITAN"
     gene            complement(4344..5390)
                     /locus_tag="PKL17_10405"
     CDS             complement(4344..5390)
                     /locus_tag="PKL17_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNK45186.1"
                     /translation="MKWSLASFLLCLSTSAAAQTTDPGYFVTRSTTAMGTVVQARAYL
                     PVRPSAPKEREVASQLDAAIAEIRRLEGLMTTWSPDSDISRVNAAAGQKAVVVSPEVY
                     DVIAKAQEFSARSSGAFDISFYALRGLWFFDEEPRAQIPDPKALAARLPLINFRAIVL
                     SEKARTVKLQKPGMAINLGGIGKGFAVDAAVKILRARGITDGMVQAGGDLMLFGSKSG
                     APFKAGIRDPRSLDPSDYFAVCEIKDHAFSTAGDYERSFIREGTRYHHILDPRTGQPA
                     RGARSVTVFAKDATTADGLDDIVLILGPERGLRIVESYLDAGAVVVDANNRVHISKSL
                     ATLCSVIHPPTPGV"
     gene            complement(5457..6206)
                     /locus_tag="PKL17_10410"
     CDS             complement(5457..6206)
                     /locus_tag="PKL17_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNK45187.1"
                     /translation="MSETTSTDPTVLETAQGPDGETLELRRVKDGFAITANGRVIIDS
                     ALRRSERELINVGMVPLRDRQDITVLLAGLGMGHTLAALLESPRVIRVDVVEHSSAII
                     DWNRSHLGTLHKASPLDDPRVHVHNMSLDKYLRALRYKSLPDVKIEGDGYLAVILDLD
                     EGPSQLLRPKNADLYSDDGLQDLEEALRPGGVLALWSAQRETDLMERLKGRYQNIAEI
                     VVPVDIPMSAGLDYIYRARRRPPVSRVRAQA"
     gene            6396..8228
                     /gene="typA"
                     /locus_tag="PKL17_10415"
     CDS             6396..8228
                     /gene="typA"
                     /locus_tag="PKL17_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNK45188.1"
                     /translation="MPKRSDVRNIAIVAHVDHGKTTLVDGLLKQAGSFRRGEVIHECA
                     LDNNELERERGITILSKCTAITYKGTRINIVDTPGHADFGGEVERVLMMVDSVLLLVD
                     AFEGPLPQTRFVTKKAFQLGLRPIVVINKIDRDGCNPQATLNAVFDLFCALDATEEQL
                     DFPVIYASGRNGYAIKELTDEKKDLTPLLDMIVQSVPAPEADVEAPLCMQVATLDYDD
                     YLGYVAIGRIGAGKIAMGDRVLLSHKNQNEQFRVQKLLAFQALKRFEIGEASAGDIVA
                     VTGMSELNVGETITSIESPKILPMLTVDEPTITMQFIANNGPFAGSEGKFVTTRNIRE
                     RLWKELKSNVALRVEETESADTFKVSGRGELHLSVLIETMRREGFEMCVSQPQVIFRE
                     GPEGKMEPYEDLLVDVDDPFVGVVIEKLGRRAGRMRDMGPSGQGRTRIEFVIPSRGLI
                     GYRNEFLTDTRGTGIINHSFREYAPFAGVLKGRPNGVLIVQDPGETNTYALFYLQERG
                     ILFAGTGERVYSGQIIGQHSRDNDLIVNPNKTKKLTNIRTTAADEKLFLTPPRIMSLE
                     AALEFINDDELVEVTPKSIRLRKRYLDHNTRKRFEKVAAAVAED"
     gene            complement(8229..9206)
                     /locus_tag="PKL17_10420"
     CDS             complement(8229..9206)
                     /locus_tag="PKL17_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45189.1"
                     /translation="MNKRSWQKLVAVLAISFVSTSNQTEACAHARTGPGAKTPPAADP
                     APTIAKVDRPTEQVWSGQNSWTAEREAQYSAFISALGTNIAQNRCRTLAACLNDPAVN
                     PLPESFGRPLHFRADCADVPYILRAYFSYVHDLPFRFTKEMVGTGRDARYYRNAQPKG
                     VRTFRDFETPRKLFEHLSSAVHSGFFRSTHTVEESDFYQTQISRLAIRPGTMFYDPNG
                     HVLVVYGLRPDGSVMTFDGHPDGFLTHGVLSERNQRGGESQGGGFKNFRPLRWDKELL
                     AQKRNQELADFGGGMQFDRTRYVVDGLPVSYHDWVRAQLQDRTAQAAAM"
     gene            9299..10810
                     /locus_tag="PKL17_10425"
     CDS             9299..10810
                     /locus_tag="PKL17_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45190.1"
                     /translation="MRHGGFLRLRGTLPRLLAVGVAAVGVWAGSEWKAPLSQSWPAER
                     SWNDKLEDEYSEFVFRIGQAVAQRRCHHLDSCLRDPAANILFEAQTDPDLVLGVDCGD
                     LPYVLRGYFSFKKHLPFGFVCGVEGIGEHVRYMSQVTPTKFCSWQSYKTPRDVLVSMS
                     NAVHSGMYRMAPEVESADFYPTALDRRGVRPGTVYYDPNGHVLTVAEVRKDGSVLLMD
                     GHPDGSLTWKRFGAAFALGGRGQGGGFKNFRTQALEGKEIRRARNEHLPLFDGQTQWD
                     RSLWPQIGGQGSYHALVKQRLSLVTTPDIESDFRESLRALCRDVQERIDSVQEAVQAG
                     LSHKAHPGELPQNIYGALGEWEQYATPSRDARLRAAVREMYDLTTSVAKDSPLLRSLG
                     NVWQTESAKPDCRFSYVNSEGKSVAIGIGTVLSRIYALSFDPYHCPELRWGARPDSEE
                     GRSCPIDAIKLQFYEKEQRLRQRIDREYGAKTPLQSGPEIAAPIDFRALLGVE"
     gene            10896..11402
                     /locus_tag="PKL17_10430"
     CDS             10896..11402
                     /locus_tag="PKL17_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45191.1"
                     /translation="MKTLMGVLFAVAMVFSSGTASADAGKCSFDSDCGAGVKCRSGRC
                     ATAAGGTCSFDSDCGGGKCRSGKCSNSPDGSCSFDSDCGEGKCRSGKCSAAAGSCSFD
                     SDCGPGVRCRSGKCATADGGSCSFDSDCGGAKCRSGKCANSPGGSCSFDSDCGAAGKC
                     RSGKCAGK"
     gene            11411..12388
                     /locus_tag="PKL17_10435"
     CDS             11411..12388
                     /locus_tag="PKL17_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNK45192.1"
                     /translation="MTRRWLRLCGLWVGLWLCGCRSVDVAALDAKADAAQPADLAQAQ
                     PADDGAVPRGDLRAVTFNTHLFFDLVCDSGRCGPTDFEQVPSAQAFAEKADAVALAVR
                     RISGDVVALQEIENQSALDAIWGRLRDVYPTAVLGETGLPASIDVAVLSRGTLLEVRR
                     HHDRVLTRPDGTKTTFSRELLEVHVDLRGQRLVMFAAHFRSKSSDDPGRRLAEAQATA
                     DIVSQTAKEFPLAVIILGGDLNDTPGSPPLTALESVASLLRVAKDRPLSSCATYFWNG
                     ELQAIDHLFVWQNARVGYVAASANAQHDATRFGLAGSDHAALLADFALH"
     gene            12396..13037
                     /gene="greA"
                     /locus_tag="PKL17_10440"
     CDS             12396..13037
                     /gene="greA"
                     /locus_tag="PKL17_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNK45193.1"
                     /translation="MPASNDSKSKTPEKNYITPIGFRRLQREFETLRGPTRREVVAKL
                     SEAAAEGDRSENAEYIYRKRQLRQIDSRIRHLIKRMAIAEVIDPSHHKGERVTFGCVV
                     TLEDDNGKMFRYQIVGVDESDPKEGRISYRSPIGRALIGKYVDDAVSVQVAAGTRELT
                     ILSLEFPSGEVGESGGETIADAMLAAQQALAESPSVDGDEDAEGSGENAEAEA"
     gene            13041..14081
                     /locus_tag="PKL17_10445"
     CDS             13041..14081
                     /locus_tag="PKL17_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNK45194.1"
                     /translation="MFPRGITFDDVLLVPGYNGIRSRQAVTTSVQITDALSLKIPVVS
                     SNMDTITGWQMAASMAKLGGLGILHRFMSIEQNVAEYRRAREFGPVGISLGVSGDAME
                     RAEALVSAGASILCVDVAHGHSKMVNQTVRQLRDRFGDNVCIIAGNVATYAGADYLSA
                     AGADVIKVGIGPGSVCTTRIKTGFGVPQLSAILDCRKVDRPLIADGGIRTPGDAVKAL
                     AAGAACVMLGGMLSGTDETPGERIEKKSSDGRSTFVKVFRGMASREAQEDYMGAMADW
                     KTAEGISIEVPAKGAVSDVISDVMGGIRSGMTYCGAKDLKDLQRKAQFMEVSRAGQDE
                     GLPHAVLRSTNR"
     gene            complement(14124..15179)
                     /locus_tag="PKL17_10450"
     CDS             complement(14124..15179)
                     /locus_tag="PKL17_10450"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNK45195.1"
                     /translation="MRSVVKASGFVVGEHLVPNARFEKIMDTSDQWIRERSGIEQRYF
                     VADGTSTSDLGARASKIAMEQAGVSPQDIDYVVFATMTPDHYFPGCGSILQNKLGLRN
                     IPALDIRQQCSGFVYALQVSDALLKAKLAKTLLLVGAETHSPFMPFSDWPTILGLADN
                     SPTDEQRALHTQVRDRAVLFGDAGAAIVLQREEDETERGLIDFELHADGSGYNKLYVP
                     SGMAHRPYVSPDMIQRLDHVPHMDGKYVFRMAVTKMPEVVRSLLGKHGLTVDQIDHFI
                     FHQANLRINEAVQQSLGIRPDRIYNNIQKYGNTTAATIPIAYDECRRSGRIKRGDLLC
                     FVGLGAGFHWGAALLRD"
     gene            complement(15261..18701)
                     /locus_tag="PKL17_10455"
     CDS             complement(15261..18701)
                     /locus_tag="PKL17_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45196.1"
                     /translation="MGAARCIKCGSENPKEALFCTACGQALHHAQNDPLVGQTIGGRY
                     HLVERIGQGAAGTIYRAEHTTLRRRMAVKLLHQQLAQNEDAVERFRREAITVCEIDND
                     HIPQVFDFGRAEDGRLFFAMEFLDGVPLSMVMEREGRLTPERTTDILTQIADGLMEAH
                     TLGYIHRDLRPRSIFLTRRRGRADFVKILDFGLAKLVQPELTDAQRTAMGMTYGDPRY
                     MAPEQARGEIVDRRADIYSLGVLGYEMLCGEPPFKGQGTFDVLSKLLEAPVPRLRDKR
                     PDCPPWLESVIRTALAKKPAERFQTVAQFLDAMERKNVLANQPPLPMQVSPGAKAEAM
                     KKLTPLNPQPDIAAKGQEVRAFVSSPAMKAISVPVEDPPRAIAQSTQVMPTVAPGTRA
                     AALAAAVAPETPVQHEQPQSIKKTLAYPAIRVGARPEPAVPAPTVAPAPVVAVQPVAS
                     PAPEKTAPTKSAAPISAKTQAPAKSTAPLKSGFVNIVATTIERGDPTPRTARAMVEQE
                     SLAWEEAALPALLTNEVDLTESATSRELTKMHPSADTPVEEMNFDAGKAASDDAVDDT
                     IVQRPSSPAPVAAASGTAAVAAPAAKQPSGPTRAEPSKAATPREPMAKEPSGPAAQRD
                     SSRIAAMATPSPVAGREPSGPAAKRVETARPAAQVGSPSPGIEIPRAKSASASSEAAP
                     LSTPALGRHTQPGIGPVTPAGQAALPVLEVAPEASMFPPSEGAENDEDATVPNLKNAE
                     THRVEKTSGDSQSSETVKHPKLDAASVHEETAKVAKVAPETAKPQTESEKTPESPPPE
                     MAHEADAGWFAQSAPAALADDDEAISLKKKTPRWVVPAVISSAALLGLLFWAQSGPSK
                     NTTTPADPPAAVTPVAETKPAVPTPSEKPPEPKVEVAALPTATALPTDNKPAPTEPPA
                     AKLEPKPEVKAEPATVAAAKTEAPAKPVPKAEPLPVKAEPKAEPKPEPKPEAKAETKP
                     AVAKAEPKPEAKPAAAKPEPKADPKPTIAKAEPKAAAAKPEAKAEEAAPAKSGAEAAQ
                     LVSLGKQKLDDGDVDAAVASFKRAVQLEPKNVDAVAGLGEASFEQGNFDVASKYLQQA
                     SKLAPRKASYLELLAQSQFKLGRYKEASDTCRKILKDHPGSGRAKQTLEQAEKKLDDM
                     "
     gene            complement(18766..19311)
                     /locus_tag="PKL17_10460"
     CDS             complement(18766..19311)
                     /locus_tag="PKL17_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45197.1"
                     /translation="MRLTFCLAVGLAFFSIGCTTANPDYPDMSACTLGERRCDSRDRA
                     IAMVCGRNADDRQVFLEEPCPLQSLCDSGRCLPPSGARTCQNFSDCSAGEVCTVLVSS
                     SATTLATYCVPAQPGAVDASMPCTKDTDCATYRCLQQTQGKFCMKACGADGVCGGARR
                     CLSLSITVTGVQGQVQTCSAP"
     gene            complement(19333..>20156)
                     /locus_tag="PKL17_10465"
     CDS             complement(19333..>20156)
                     /locus_tag="PKL17_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45198.1"
                     /translation="GADFTVLSGEEVRQRLSGSADALNCQKGGCIAQASSQLQLDRVI
                     VAQITMKTAVGGSAYQIRLTAIDPTGKQGEISYAGRCGDEGEGCNLTRAFESMRKTSM
                     ELLPKLKPAAAPPVEVAKTEPVAQPVPTSAPSPEGTLKDPSSSPMQTTTAPAVSTSQP
                     ATPHTRNTGYRIGWIASGIVAGGLLITSIPFLILSPRDGQPTCTDRPSNQCPTRYQGN
                     LATGLGLLGGGVAAATAFGVLYYLDRRDLKRSQGQVAVLPFYFESGSGLAAVGRF"
BASE COUNT         3668 a         5673 c         6644 g         4171 t
ORIGIN      
        1 ggcgaaaagt gcgaaaagtc gaaggtcagc ttgtcgggac cgacgtagct gcctttttgg
       61 gtgacgtgct gcccgagcac ttcgcgcagg gcatgatgca aaaggtgggt cgccgagtgg
      121 ttgcacctgg tgcgatcgcg gcgctcgcca tcgaccagca gcgacacctc atccccgaca
      181 aacaggctgc cggacacaac gcgcgctttt tggacaatca gctcggggac cggcttccag
      241 gtgtcggtga cttcgacttc ggccacggtt tcgctgccca cgattttgac gatgcgaccc
      301 gtgtcgccgg cctgaccgcc ggatcggcca tagaacgggg tgcggtcggt caggacttcg
      361 acttcttggc cggcagcggc tgcgtcgacg ctttgacccc cgacaatcag ggccaagatg
      421 cggccttccg cacaaatgcc gtcgcgcccg tgcgcatagc caaggaatgt cgttccgcca
      481 tgggcggcgc gcagggcgtg gtaggcatgc gaaacggcct ggtcgcccga accggcgaac
      541 ttgctgcgtt cgcgctgctt gcgcatctcc tctgcaaagc cgtactcatc gacgacaaag
      601 ccctgctcgc ggccaatcac ttgcgtcagg tcgagcggga agccgtaggt gtcgtgcagc
      661 tggaacgcga ccttgccagg cagggttttt tggccggcag tgtcggcggc aaactcggtc
      721 tcgctgatca ggcgcaggcc gcggtcgatg gtgctgcgga agccttcgtc ttcggcgttg
      781 acggcttttt cgatcaggct ttcggctttg cgcagctctg ggtagatgtc cccgagcagc
      841 ttggtgactt ccaggcacag gctggcgaag aacccagccg ggagcccaag tcggacggcg
      901 aagcgaatcg cgcgccgcat gatgctgcgc agcacgtagc cgcgggacag gttggacggc
      961 agcacgccat cggcaatcag gttggcggtg gcgcggctgt ggtcggcaag aacgcgcatg
     1021 ccgacgtcgt catcgctgtc tgtgcggcgg tagggtttgc cgcaggccgc cgccacggtt
     1081 tccaaaagcg ggataaacag gtcggtgtcg tagttcgatc gctgcccgat ggcgacggca
     1141 gtgacgcgct ccaggccggc gccggtgtcg atgctggggc ggggcagcgg gaccagcggg
     1201 gcgtctttct gcgcccgctc aaactgcata aagaccaggt tccagatttc gacccagccg
     1261 ctgccatcgg ggccgggttc gtcgccaaag cgcgacaggt cggcagcccc atcgcccagc
     1321 cagtagtgga tttccgagca cggcccttgc ggtccggtgt cgcccatcat ccagaagttg
     1381 tctttcatgc ccagaccgaa gatgcggtcg tcaccgtacc cggacagctt gcgccacagc
     1441 tcgcgggctt catcgtcggg ccccagcttg agaccactgt cgccggcgaa gaccgacacg
     1501 cacatccgct tgggatcaag cgccagctct ttggtcagaa attcccaggc aaacaggatc
     1561 gcgtcggtct tgaagtagtc gccgaacgaa aagttgccga gcatttcgaa gaaggtgtgg
     1621 tggcgggccg tgcgaccaac attgtcgagg tcgttgtgct tgccgccagc gcggacgcac
     1681 ttttgtgacg tcgaagcgcg cttgtagtcg agctgctttt ggccggtaaa cacgtctttg
     1741 aactggacca tgccggcgtt tgtgaacagc agggtcgggt cagacggaac caggggcgag
     1801 gagcgcacga cttcgtggcc ctgcctgcgg aaatagtcga gaaacaggct gcggacgcgg
     1861 gtggatgctt gggcacttgc gccaatttgg gaagacatgt gcccgactat atcacgcccg
     1921 gttcgatggc aaacccaggg gtcgtgggtc cgcgctaaga tagaccgggg ccacagggtc
     1981 tttgccagga ggtcgactat gtacgggatg tttcgacgaa cacttattgg aatcgcttgc
     2041 cttggactgg gttctttgtc tgcacacgcg gctgtcacac tgccgaacgg acagtcggtt
     2101 cctgttggta cagcgctaca gggctatctg aacggctcgg cgaacaacaa caacatcaac
     2161 gagatgatca atgcgcagca ggatgcgcaa gtcgagccgc agaagttttc gcccctgtgc
     2221 gacttttcgg ggcgctacat cgccaagggg ggcggcgcca actttgcgat tggctggtac
     2281 aacgtcgacg acgcccgcgc gagcaacaac ccgccaaagt atgtgccggt cgataacggc
     2341 gcgaatctga ataccgcggc accgaccagc gacatccaga tcctgttccc attttcggcg
     2401 aatttgcctc cggccaatat gctcgacctg acggcggctt cgattcgtag caatgccaag
     2461 tacaaaggcg gcctgattgg ctttgttctt gtgccaaatc ccaatggcac gggcgctggt
     2521 gccacccaat accactacac cgagcaccgc tttaacgtgt actgctcgaa gtgcaccacg
     2581 ccggggccgt ggtattcgac gctgatttac aagtcgaacc agctcgcgaa cacgttttat
     2641 ttgggatttg aagacctcga cttcattgat gcgacgggca ataccggcgt aaacggcaac
     2701 gacctcgact acgaggactt cctgtttcgc ttcacgggtg tggtctgctt gggcgcaggg
     2761 cagccatgca ccgacagcac gcagcagggc gcttgccaga ttggcgtcac cgagtgcgac
     2821 gcgcagggca agattgtctg caaggcgatc gtaaagccca accaaaacaa ggacatctgc
     2881 gatggtgtcg acaacgactg caatggtctg gtcgatgaca acgcgacgtg tccggtcggt
     2941 cagatttgtt ccaaaggcgg ctgctctgca ccgtgcggca gcgaagtgct gtgtccggtg
     3001 ggccagcagt gccagatggg tcgctgcatc gatgacgctt gcgtcggcaa gacctgcgcc
     3061 gccaaccaag tctgccgggg cggaagctgc gtaagtccct gctcgggcat caagtgtccg
     3121 tcgccgtaca tctgctcggg cggaagctgc attgaccctt gcgtcggtgt gacctgcggc
     3181 cagggcaaga cgtgttcaaa tggcgcctgc atcacgacgt gcgactgcct gccatgcagc
     3241 agcggtcagg cctgcttgtc gtcgactggc aagtgcgtcg attcgggatg tgaaaagatc
     3301 acctgctcag cggggcaggc ctgcgtcaaa ggaagctgcg tcgatgcctg cgcgggcgcg
     3361 gtgtgcccca ccggacagat gtgcacggcg ggcaagtgca tcgacattcc gcccgatggt
     3421 ggtggcaatg tggacgccgg caccgacccg acaccgattg acggtggcag catttccacc
     3481 ggctgcacct gccgctttgc gccggcgcag aacggttcga gcgcgtgggg aattcttgcc
     3541 ggcctgggtc ttttggcggt ctcggcccgg cggcggcgtc gtcgatccta gttcgccgtg
     3601 atctggagct tgaagcgtcc cggtccggcc ttggaattga tgagcaggtt gtaggtgccg
     3661 gccttgggac gcaggtagtg gaactctagg gtgctcggct tgtcttgtcc ggcttggaag
     3721 gtcagccccg gatcacttgt aaacgaaaac gcctctcgcc ccagcatctt ctgctgcgag
     3781 ccatcgagct gcgcaaagta caggttgaag ctgtttagat cgtttcgcag cacgagggtg
     3841 ccatcaatgt cgccgctcgg ctgaagcttg aacgtgccgg ctgccgacga tatttcgtta
     3901 taagcaagct gctggacttc ttcgacaaac acgctgatgc ggtcgacatc gccgctgtcg
     3961 ttgtaggcga taagcttggc ttcgccgacg tgcatgccgg tcaggatgta gctgcgggcg
     4021 gtcggttcct cgatcttgag gacggcggga aggtcggtgc ggaccgacag aagcgagttg
     4081 tcgctcttgg ctgtcgcgat caagtcgatt ttgcccgagg acgcgagctt ggcgctcagc
     4141 ggacagcctt gcagatcact taggacgccg ccgcagttgc ggacgacgaa cgtggcgcgg
     4201 cccagctcgc cgtcgttttt gctggaatcg caggcggaca ggctgatgca ggcgaggagc
     4261 gcggagccaa ggagggaaac atatcgcgag gtcggcatgt ggcgaggata gcccaggcca
     4321 cagcttgtgt gcggtcgcag gtgttacacg ccaggggttg gtgggtggat gacactgcaa
     4381 agggtggcga ggctcttgct gatgtgaacg cgattgttcg catcgacgac gacggcccct
     4441 gcatcaagat aggattcgac gattcgcagg ccgcgttccg ggcccaagat gaggacgatg
     4501 tcgtcgagtc catcggctgt cgtcgcatcc ttggcaaaca ccgtgacgga gcgggcgcct
     4561 cttgcgggct gtccggttcg cgggtcgagg atgtgatggt agcgagtgcc ctcgcgaatg
     4621 aaggatcgtt cgtagtcccc ggcggtcgaa aaggcgtggt ctttgatctc gcagacggca
     4681 aagtagtcgc tgggatcgag gctgcgcggg tcgcggatgc ccgctttgaa cggggcgccg
     4741 cttttgcttc caaacagcat cagatcgccg ccagcctgca ccatgccgtc tgtgatcccg
     4801 cgggcccgca aaatttttac ggcagcatcg accgcaaagc ctttgccaat gccgcccagg
     4861 tttattgcca tgccaggctt ttgcagcttc acggtgcggg ctttttcgga cagcacgatg
     4921 gcgcgaaagt tgatgagcgg caggcgggcc gcaagggctt tgggatcggg gatctgggcc
     4981 ctgggttctt cgtcgaaaaa ccacaggcca cgcagcgcgt aaaaggagat gtcaaaagca
     5041 ccgcttgaac gggccgaaaa ttcctgcgct ttggcgatga cgtcatagac ctcgggcgat
     5101 acgacgacgg ctttctgtcc agccgcggca tttacgcgcg agatgtcgct gtctggactc
     5161 cacgtcgtca tcagtccttc caggcggcgg atttcggcaa tcgccgcgtc aagctgactt
     5221 gcgacctcac gttcttttgg cgcagacgga cggaccggca ggtaggcgcg cgcctgcacg
     5281 acggttccca tcgcggtcgt cgatcgggtg acaaaataac cgggatcggt tgtctgcgcc
     5341 gctgccgacg tagacaagca cagcagaaaa gaagccaaag accacttcac gcggctaccc
     5401 tagcacgggt tttcgcttgg tcgcgccccg ctgagcgggc cggaaggtcg ggtcgtttat
     5461 gcttgggcgc ggacgcggga cacgggcggg cgacggcggg cgcggtagat gtaatccagg
     5521 ccggcgctca ttgggatgtc cacggggacg acgatttcgg cgatgttctg gtagcggcct
     5581 ttgagccgtt ccatcagatc ggtttcccgc tgtgccgacc acagcgcaag gacgccgccc
     5641 ggacgcagcg cttcttccag atcctgcaat ccatcgtcgc tgtagaggtc ggcgttcttg
     5701 ggtcgcagaa gctggcttgg gccctcgtcg agatcgagga tgaccgccag atagccgtcg
     5761 ccttcgatct ttacgtcggg cagcgacttg taacggagcg cgcgcaggta tttgtccagg
     5821 ctcatgttgt gaacgtggac gcgcgggtca tcgagcggcg acgctttgtg cagcgtcccc
     5881 aggtgcgagc gattccagtc gatgatggca gaagaatgct ccacgacatc gacgcggatg
     5941 acgcgcggac tttccaaaag agcggccagc gtgtgaccca tgccaagccc agcgagcagc
     6001 accgtgatgt cttggcggtc gcgcagcggc accatgccga cattgatgag ctcgcgttcg
     6061 ctgcggcgca aggcactgtc aatgatgacg cgaccgtttg ccgtgatggc aaagccgtct
     6121 ttgactcgcc gcagctccaa ggtttcgcca tcagggcctt gggccgtttc taggacggtg
     6181 ggatcggtgc ttgtcgtctc tgacatgaga gtcggggctc ctgggcgttt gaaacaattg
     6241 cgaaacggaa gtggtgattg tcctcttcgc tttggctggc tgtgctatag agccgcaccg
     6301 cgcaaacaaa ttgagaggca gtcatcgtat catgctgggc tgcttccctt ctacgcccgc
     6361 cgcagcgccg atttgaaccc gaggcctttt gcaacatgcc caagagatct gatgtccgca
     6421 acatcgccat cgtcgcccac gtcgaccatg gcaagaccac cttagtcgat ggcctgctca
     6481 agcaggccgg ttcatttcgt cgcggtgaag tcatccacga atgcgcgctc gacaacaacg
     6541 agctagagcg tgagcgcggc atcaccatct tgtcgaagtg cacagcgatc acctacaagg
     6601 gaacccggat caacatcgtc gatacaccgg gccacgccga cttcggtggt gaagtcgagc
     6661 gcgtgctgat gatggtcgac tcggttctgc tgttggtcga cgcctttgaa ggtccgctgc
     6721 cgcagacccg ctttgtcacc aaaaaagcct tccagcttgg gctgcgtccg atcgtggtca
     6781 tcaacaagat cgatcgcgac ggctgcaatc cgcaggcgac gctcaacgcg gtgtttgatc
     6841 tgttctgcgc gctggatgcg acggaagaac agcttgattt ccccgtgatc tatgcgtcgg
     6901 gccgcaacgg ctacgcgatc aaagagctta cggacgaaaa gaaagacctg acgccacttc
     6961 tcgacatgat cgtgcagagc gtgccggcac cggaagcgga tgtcgaagcg cctttgtgca
     7021 tgcaggtggc gacgctcgac tatgacgact acctggggta cgtcgcgatt ggtcggattg
     7081 gcgccggcaa gatcgcgatg ggtgatcggg tgctgctcag ccacaagaac caaaatgagc
     7141 agttccgggt gcagaagctc ctggctttcc aagcgctcaa gcggttcgag attggcgagg
     7201 cgagcgcggg cgacatcgtt gccgtgaccg gcatgagcga gctaaacgtc ggcgagacga
     7261 tcacgtcgat cgagtcgccg aagattctgc cgatgctgac ggtcgatgaa ccgacgatca
     7321 cgatgcagtt tattgccaac aacggcccct ttgcagggtc ggaaggcaag ttcgtgacga
     7381 cccgcaacat ccgtgagcgg ctatggaaag agctgaagtc gaacgtcgcg ctgcgcgtcg
     7441 aggaaaccga gtcggcggat accttcaagg tgtcgggccg tggggaactc cacctgtcgg
     7501 ttctgatcga gacgatgcgg cgcgaaggct ttgagatgtg cgtgtcgcag ccgcaggtca
     7561 tcttccgcga agggccggaa ggaaagatgg agccctacga agacttgctg gtcgatgtag
     7621 acgatccgtt tgtcggcgtg gtgatcgaaa agctcggtcg gcgggccggt cggatgcggg
     7681 acatgggccc atcgggacag ggccggacgc ggatcgagtt tgtgattccg tcgcgcggtc
     7741 tcatcggcta ccgcaacgag tttttgaccg atacgcgcgg caccggaatc ataaaccaca
     7801 gcttccgcga gtacgcgccg tttgccggcg tgctcaaggg gcggccaaac ggcgtgctca
     7861 tcgtccaaga tccgggcgaa accaacacct atgcgctgtt ctatctgcaa gagcgcggga
     7921 ttctgtttgc cggcacgggt gagcgggtct actccggaca gatcatcggt cagcactcac
     7981 gcgacaatga tctgatcgta aacccgaaca agaccaagaa gctgaccaac atccggacga
     8041 cggcggcaga tgaaaagctg tttttgacgc cgccgcggat catgtcgctc gaagctgcgc
     8101 ttgagttcat aaacgacgat gagctggtgg aagtgacgcc caagtcgatc cgcctgcgca
     8161 agcgctactt ggatcacaac acccgcaagc gcttcgaaaa agtcgctgcc gcagtcgccg
     8221 aagactagct acatcgcggc ggcctgcgct gtcctgtctt gtagctgggc acggacccag
     8281 tcgtgatagg aaacaggcag tccgtcgacc acgtagcgcg tccggtcaaa ctgcatcccg
     8341 ccaccaaaat ctgccagttc ctgattccgc ttttgcgcca gcagttcttt gtcccagcgc
     8401 agcggacgga agttcttaaa accaccgccc tgactttctc cgccccgctg gttgcgttcg
     8461 gacagaacgc cgtgggtcag gaacccatcg gggtgcccat caaaggtcat gacggaaccg
     8521 tctgggcgga gcccgtacac cacaaggaca tgtccgttgg gatcgtaaaa catggtgcca
     8581 ggacggatgg ccaatcggct tatctgtgtc tggtaaaagt cggattcttc gacggtgtgt
     8641 gtggaacgga agaagccgga atggacggcg ctgctcaggt gctcaaacag cttgcgcggg
     8701 gtttcgaagt cgcggaacgt gcggactcct tttggttgtg cattccggta gtagcgggca
     8761 tcgcgacctg tcccaaccat ttctttggtg aagcggaagg gcaggtcgtg aacatacgaa
     8821 aaataggcgc gcaagatgta cggaacgtcg gcgcagtcgg cacgaaagtg cagcggtcga
     8881 ccgaaggatt ccggaagtgg attcaccgcg gggtcgttca agcatgcggc gagcgtgcgg
     8941 cagcgatttt gcgcgatgtt tgtgcccagc gctgaaatga aggcgctgta ctgggcttcg
     9001 cgctcggcgg tccaggaatt ctgtccgctc cacacttgtt cggtgggtct gtctactttg
     9061 gcgatggtgg gggcggggtc cgctgcgggc ggagttttgg cgccaggccc agtccgggcg
     9121 tgcgcgcagg cttctgtttg gttggacgtg gagacaaagg aaatggcgag cactgcgacc
     9181 agtttttgcc agcttcgttt gttcatatcg atcagtgtta ttctcgatcc ggcaaacgga
     9241 agtccggtcg gacgattatt ttttcgtcag tgagccgcgg gataaggtgc gatagccgat
     9301 gcgacacggg ggatttctgc gtcttcgcgg tacgctgcca cggcttttgg cagtgggggt
     9361 tgctgcggtc ggcgtgtggg caggatcgga atggaaagca ccgctttcgc agagctggcc
     9421 ggcagagcga agctggaatg acaaactgga agacgagtat tccgagtttg tttttcgcat
     9481 cggacaggcg gtcgcgcagc gtcgctgtca tcacctggac tcctgtctgc gcgatccggc
     9541 ggcgaacatc ctgtttgaag cgcagaccga tccagacttg gtgctggggg tggactgcgg
     9601 ggatcttccg tatgttctgc ggggctactt ttcgttcaaa aaacacctgc cgtttggctt
     9661 tgtctgcggc gtggaaggaa tcggcgagca cgtccgctac atgtcccaag tcacaccgac
     9721 gaaattttgt agctggcaaa gttacaagac accgcgcgac gtacttgttt cgatgagcaa
     9781 tgcggtgcat tccggaatgt atcggatggc tccggaagtc gagagtgcgg atttctatcc
     9841 gacggcgctt gatcgtcgcg gggtgcgtcc agggactgtg tactacgacc caaacgggca
     9901 tgtcctgacc gtggcagaag tgcgcaagga cggcagtgtg ctgctgatgg acggacatcc
     9961 ggatgggagt ctgacttgga agcgctttgg ggcggcattt gctcttggcg ggagaggcca
    10021 aggcggcggg tttaagaact tccgaaccca agctctggaa gggaaagaga tccgccgtgc
    10081 tcgcaatgaa cacttgccgc tgtttgatgg gcagacgcag tgggatcgca gcctgtggcc
    10141 acaaatcggc gggcagggga gttatcacgc gctggtcaaa cagcggctgt cgcttgtgac
    10201 gacgccggac atcgaaagtg actttcgcga atcgctgcgt gcgctgtgtc gcgatgtgca
    10261 ggagcggatc gattccgtac aggaagcggt gcaggccggg ctatcacaca aagcgcatcc
    10321 gggggagctg ccacagaaca tttatggagc gcttggggaa tgggaacagt acgccacacc
    10381 gtcgcgggat gcgcggctgc gtgcggcggt gcgggagatg tacgatctga ccacttcggt
    10441 ggccaaagac agtccgctct tgcgttccct tgggaatgtc tggcaaacgg aaagcgccaa
    10501 accagactgc cgtttttcct atgtgaattc agagggaaag tcggtggcca tcgggatagg
    10561 cacagtgctt tcgcgaatct acgcgctcag ttttgatccg tatcactgtc cagagctgcg
    10621 ctggggggca cgaccggata gcgaagaggg tcggagctgt cccatcgacg ccatcaagct
    10681 gcagttttac gaaaaggaac agcggctgcg gcagcggatc gatcgggaat atggcgcaaa
    10741 aacgcccctg caaagtggtc cagagatcgc ggcgccgatc gactttcgcg cactgctggg
    10801 agtcgaatag gaaaccagaa catctcgtct gcttgtgtta cggttttgct gcgtttcgtt
    10861 ttggcaaaac cagcaacgac aaaagaggaa acacaatgaa gacgctgatg ggagttctgt
    10921 ttgccgtcgc gatggtattt tcatcgggta cggcttcggc agatgccggc aagtgcagct
    10981 ttgattcgga ctgtggcgcg ggggtcaagt gccgcagcgg acgctgtgca acggcggctg
    11041 gcggaacctg tagctttgat tcggactgcg gcggtgggaa gtgtcgaagc ggcaagtgct
    11101 ccaattcgcc ggatggatcg tgcagctttg attcggactg tggtgagggc aagtgtcgaa
    11161 gcggcaagtg ctcggcggcg gcgggttcct gttcgttcga ttcggactgc gggccggggg
    11221 ttcgttgccg aagcggcaag tgcgcgacgg cggatggcgg ttcctgttcg tttgactcag
    11281 actgtggtgg ggccaagtgc cgaagcggca agtgcgcaaa ctcccccggt gggagctgct
    11341 cgtttgattc ggactgcggt gcggctggca agtgtcgcag cggcaagtgc gcaggaaagt
    11401 aggccggctt gtgacgcggc gttggcttcg gctgtgtggg ctgtgggtgg gtttgtggct
    11461 ctgcggctgc cggtcggttg acgttgcggc gctcgatgcc aaagccgacg cggcgcagcc
    11521 tgcggatctg gcacaagcac agcctgcgga cgatggcgcg gtgccgcgtg gcgatctgcg
    11581 cgcggtgacg tttaacaccc atctgttttt tgatctggtc tgtgattcgg ggcgctgcgg
    11641 gccgacggat ttcgagcagg ttccgtctgc gcaggcgttt gctgaaaaag ccgatgcggt
    11701 ggcgttggcc gtgcggcgga taagcggcga tgtggtcgcg ctgcaagaga tcgaaaacca
    11761 gagcgcgctc gatgcgatat gggggcggct gcgcgacgtg tatccgacgg cggtgcttgg
    11821 ggaaacgggc cttccggcgt cgattgacgt cgcggtgctg tcgcgcggca cactgctaga
    11881 agtccggcgg catcatgatc gcgtgctgac ccgaccggac ggaaccaaga cgacattttc
    11941 gcgtgagctt ttggaagttc acgtcgatct gcgcgggcaa cggctggtga tgttcgcggc
    12001 ccactttcga tccaagtcga gcgatgatcc ggggcggcgt ctggccgaag cgcaggcgac
    12061 ggctgacatc gtttcgcaga cggcgaaaga gttccccctt gcggtgatca tcttgggcgg
    12121 ggatctgaac gacacgccgg gttcgccgcc actcaccgct ctggagtcgg tcgcgtccct
    12181 tcttcgggtc gccaaagatc ggcccctgag cagctgtgcg acgtacttct ggaacggcga
    12241 gctgcaagcc atcgatcacc tgttcgtttg gcaaaatgct cgtgtcggct acgtggccgc
    12301 gagtgcgaat gcacagcacg atgcgacgcg gtttgggctc gcaggctcgg atcacgccgc
    12361 attgctcgcc gacttcgcgt tacactagcg gcgctgtgcc agcctctaac gattcgaaat
    12421 cgaagactcc tgagaaaaac tacattacgc ccatcggttt ccgtcgcttg cagcgcgaat
    12481 ttgagacgct gcgcggacca acgcggcgcg aggtcgttgc caagctgtca gaagcggcgg
    12541 cggaaggcga ccgttccgaa aacgccgagt acatttatcg caagcggcag cttcggcaga
    12601 tcgacagccg gattcgtcac ctgataaagc gcatggccat cgccgaggtc attgatccga
    12661 gccaccacaa gggcgagcgg gtgactttcg gctgtgttgt cacgctcgaa gacgacaatg
    12721 ggaaaatgtt tcgctaccag atcgtcggcg tcgatgaaag cgatccaaag gaaggtcgca
    12781 tcagctaccg ctcaccgatt gggcgggcgc tgattggcaa atatgtcgat gacgcggtgt
    12841 cggtgcaggt tgcggcgggc acgcgcgaac tgacgatctt gtcacttgag tttcccagcg
    12901 gtgaggtggg ggagagcggt ggtgaaacca ttgccgatgc catgctcgcc gcgcagcaag
    12961 cgctggctga gagtccttcc gttgacggag atgaggacgc cgaaggcagc ggtgaaaacg
    13021 cagaagcgga ggcctaacgc atgttcccgc gtggcattac tttcgacgat gttctcttgg
    13081 tccctgggta taacggcatc cggtcgcgcc aggcagtgac cacgagcgtg cagattacgg
    13141 acgcgctgtc gctcaagatt ccggtcgttt cctcgaacat ggacacgatc acgggctggc
    13201 agatggcggc gtcgatggca aagctcggcg gcctgggaat tttgcaccgc tttatgtcga
    13261 ttgagcagaa cgtcgcagaa tatcggcggg cgcgggagtt cggtccggtc ggcatttcgc
    13321 tgggcgtgtc aggcgatgcg atggaacgcg ccgaagcgct ggtttcggcg ggggcgtcga
    13381 ttctctgcgt cgatgtcgcg catggccaca gcaagatggt caaccagacg gtgcggcagc
    13441 tgcgcgacag gttcggtgac aacgtctgca tcatcgcggg caacgtcgca acgtatgcgg
    13501 gcgccgatta tctttcggca gcgggcgctg atgtgatcaa agtcgggatc ggtcccggat
    13561 cggtctgcac gacgcggatc aagaccggtt tcggcgttcc acagctgtcg gcgattttgg
    13621 attgtcgaaa ggtcgatagg ccgctgattg cagacggtgg gattcggacg ccgggcgacg
    13681 cggtaaaggc gctggcagcc ggcgcggcgt gcgtgatgct cggcgggatg ctgtcaggaa
    13741 ccgacgagac gccgggcgag cggatcgaaa agaagtcgag tgatggcaga tcgacgtttg
    13801 tgaaagtctt ccgcggcatg gcctcgcgag aggcgcagga agactacatg ggcgcgatgg
    13861 cggattggaa aacggcggaa ggaatcagca tcgaggtgcc ggccaaaggc gcggtcagcg
    13921 atgtgatctc ggatgtgatg ggcgggattc gatcgggcat gacgtactgc ggtgccaaag
    13981 acctgaaaga ccttcagcgc aaggcgcagt ttatggaagt gagccgcgct ggtcaggatg
    14041 aaggtctgcc gcacgccgtc ttgcgttcga cgaatcgcta gagacagcgg gcgcgcggtc
    14101 cggcgcagtg tcgtaggatg ggtctagtcg cgaagcaggg cggcgcccca gtggaagccc
    14161 gcgccaagtc caacgaagca gagcagatcg ccgcgcttga tccgtccaga acgtcggcac
    14221 tcgtcgtagg caatcggaat cgtcgccgcg gtggtgttgc cgtacttctg gatgttgtta
    14281 tagatgcggt ccggtctgat tccgagcgac tgctggaccg cttcgttaat ccgcaggttg
    14341 gcctggtgga agatgaagtg gtcgatctgg tcgacggtga gaccgtgttt tccgagcagc
    14401 gagcggacca cttccggcat cttggtcaca gccatccgga acacgtactt gccgtccatg
    14461 tgcgggacgt gatcgagacg ctggatcatg tccggtgaga cgtagggccg gtgcgccatg
    14521 ccggacggca catacagttt gttgtagccg gaaccgtcgg cgtgcagctc gaaatcgata
    14581 agtccccgct cggtctcgtc ttcttcgcgc tggagcacga tcgcggcacc ggcatcgccg
    14641 aacaacaccg ctcgatcgcg cacttgggtg tgcagagccc gctgctcgtc cgtcggtgaa
    14701 ttgtcggcca gacccaggat cgtcggccaa tccgaaaacg gcatgaacgg cgagtgggtt
    14761 tctgctccga cgagaagcag cgtcttggct agcttggctt tgagcagcgc gtccgagact
    14821 tgcagcgcgt agacaaagcc actgcactgc tgccgaatgt cgagcgccgg tatgttccgc
    14881 agcccgagct tattttgcag gatgctgcca cagccgggga agtaatggtc cggcgtcatc
    14941 gtcgcaaaga cgacgtaatc aatgtcctgc ggagaaaccc cggcctgctc catggcaatt
    15001 ttgctggcgc gcgcaccgag atcagaggtt gaagtgccgt cggctacaaa gtagcgctgc
    15061 tcaatgcccg agcgctcgcg aatccactgg tccgacgtat ccatgatctt ttcgaaccgc
    15121 gcattcggaa ccaggtgttc gccgacgaca aagccagaag ctttgacaac actacgcatg
    15181 atagctcctt cgttcctcgc ctgtacggcg cggaaagtct taccttacag cagttttgtg
    15241 acagcttgtc gaatggtccg tcacatgtcg tcgagctttt tctccgcttg ctcgagcgtc
    15301 tgcttggcgc gaccagagcc tgggtggtct ttgagaatct tgcgacacgt atcggacgct
    15361 tccttgtagc gaccgagctt gaactgcgac tgggcgagca gctccagata cgacgccttg
    15421 cgcggcgcga gcttggacgc ctgctggagg tatttcgagg ccacgtcgaa gttaccctgc
    15481 tcaaagctcg cttcaccaag tcctgccacc gcatcgacgt ttttgggttc tagctgcaca
    15541 gctcgcttga agctggccac ggcagcatcg acatccccgt catcgagctt ttgtttccca
    15601 agagaaacca gttgcgcagc ttccgcaccg gatttggcgg gcgcagcttc ttcggccttc
    15661 gcttctggct ttgccgcagc ggctttgggc tcggctttgg caatggtggg tttgggatct
    15721 gctttgggtt ccggctttgc cgcagccggc ttggcttccg gttttggctc ggctttggcg
    15781 acggcaggtt tcgtttccgc tttggcttcc ggcttgggct ccggctttgg ttccgctttg
    15841 ggctctgctt tcacgggcag cggctcggct tttgggaccg gctttgcggg cgcttcggtc
    15901 ttggcggctg caaccgtcgc gggttcggct ttcacttccg gtttcggttc cagcttcgcc
    15961 gctggtggtt cagtcggcgc tggcttattg tccgtcggca gagcggttgc cgttggcaga
    16021 gcggcgactt cgacttttgg ttccggtggt ttttcgctcg gcgttgggac cgcgggtttg
    16081 gtctccgcga caggagtgac cgctgcaggc ggatcggctg gggtcgtcgt gtttttgctc
    16141 ggaccgctct gtgcccaaaa aagaagtccc aaaagcgctg ccgacgagat gaccgcgggt
    16201 acaacccagc gcggtgtttt ctttttcagg ctgatcgctt catcgtcatc ggcaagtgcc
    16261 gcaggagccg actgcgcaaa ccaacctgcg tcggcttcgt gcgccatttc cggcggcgga
    16321 gactcgggcg ttttttccga ttcggtctgc ggcttggctg tttcaggcgc gactttggcg
    16381 accttggcgg tttcctcgtg cacgctcgcg gcatccagct tgggatgctt gacggtttcg
    16441 cttgattggc tgtcgccgct tgttttttcg acgcggtgcg tctcggcgtt tttcaggttg
    16501 ggaaccgttg cgtcctcatc gttttcggca ccctcgctgg gtggaaacat cgacgcttct
    16561 ggcgcgactt ccagcaccgg cagcgcggct tgtcccgctg gtgtcacggg gcctatgcct
    16621 ggctgggtgt gccgaccgag ggctggagtc gacaggggcg cggcttccga cgatgcgctc
    16681 gcggatttgg cacgcggaat ctcaattccc ggtgatggtg atcccacctg ggcagcgggc
    16741 cgggctgtct caacgcgctt ggcggcaggt ccgcttggtt cgcgacccgc gacgggggat
    16801 ggcgtggcca tcgcggcaat gcgactggaa tcccgctgtg ccgccggtcc gctcggttct
    16861 ttggccatcg gctcgcgcgg ggttgcggct ttgctgggct cggcgcgcgt cggtccgctt
    16921 ggttgtttgg cggcgggcgc tgccacggca gccgtcccgc ttgcggctgc aacaggcgcc
    16981 gggctcgatg ggcgctggac gatcgtatcg tcgactgcat cgtcgctggc agcttttcct
    17041 gcatcgaagt tcatttcttc gaccggggtg tctgccgacg gatgcatttt ggtcagttcg
    17101 cgtgaggtgg ccgattcggt cagatccact tcatttgtaa gaagcgcggg tagggcggct
    17161 tcttcccaag cgaggctctc ttgttcgacc atcgctcgcg ctgtccgcgg tgttggatcg
    17221 ccacgctcaa tggttgtcgc cacgatgttg acgaaaccgc tcttgagcgg cgccgtcgac
    17281 ttggctgggg cttgggtttt ggcactgatc ggcgcggcag acttcgtggg ggcagttttt
    17341 tctggggctg gggacgcaac tggctggact gcgacgacgg gtgccggagc aacagtcggt
    17401 gctgggactg cgggttcggg gcgcgcgccc actctgatgg caggatacgc gagcgtcttt
    17461 ttgatcgact gcggctgctc gtgctgcact ggggtctcgg gggccaccgc tgcggccagt
    17521 gctgcggctc gcgttcctgg ggccacggtc ggcatcactt gggtggattg cgcgatggca
    17581 cgaggcggat cttcgaccgg aacgctgatg gctttcatcg ccggcgagct gacaaatgca
    17641 cgcacttcct ggcctttggc ggcgatgtcc ggctgtggat tgagcggcgt cagctttttc
    17701 atcgcttctg ccttcgcacc cggtgaaacc tgcatcggca gtggcggttg atttgccagc
    17761 acgtttttcc gttccatcgc gtcgaggaac tgcgccacgg tctggaatcg ctctgccggt
    17821 tttttcgcca atgcggtccg gatcaccgat tcgagccacg gcgggcagtc cggccttttg
    17881 tcgcgcagcc gcggcaccgg ggcttcgagc agcttgctca ggacatcaaa cgtgccctgg
    17941 cctttgaacg gtggctcgcc gcatagcatt tcgtaaccaa gcacgccgag cgagtaaatg
    18001 tcggcacgtc gatcgacgat ttcgccgcgc gcttgctcgg gggccatgta gcgcggatcg
    18061 ccataggtca tgcccatcgc ggtgcgctgt gcgtcggtca gttccggctg cacaagcttg
    18121 gccaggccaa agtcgaggat ctttacaaag tcggcccgtc ctcgtcgtcg cgtgaggaaa
    18181 atgctgcgcg ggcgcaggtc gcgatggata tagccaagcg tgtgcgcttc catgagtccg
    18241 tcggcaatct gcgtcaaaat gtccgtcgtg cgctcaggcg tcagacggcc ttctcgctcc
    18301 atcaccatcg atagcggcac accgtccagg aactccatcg cgaagaaaag tcgaccatct
    18361 tcggcgcgac cgaagtcaaa cacctgtggg atgtggtcgt tgtcgatttc gcagacggtg
    18421 atggcttctc gacggaatcg ttcgaccgcg tcttcgtttt gggcaagctg ctgatgcagg
    18481 agcttgaccg ccatccggcg tcgcagcgtt gtgtgctcgg cgcgatagat ggttccagcg
    18541 gcgccctggc cgattcgttc gaccaagtga tagcgtccgc caatcgtctg tccgacgagc
    18601 ggatcattct gcgcgtggtg cagtgcttgc ccacaggctg tgcagaacag cgcctccttg
    18661 ggattttcgc ttccgcactt gatacaacgg gcagctccca tcactagtcc tctttcctca
    18721 tcgcaaagtg cctttggcgg cggtgttgtt cggttttttg actcgtcatg gggcgctgca
    18781 agtctggacc tggccctgca cgccggttac ggtaatcgac agcgatagac agcgacgagc
    18841 gcctccgcag acaccgtcag cgccacaggc tttcatgcag aacttgccct gggtttgttg
    18901 cagacagcga taggtggcgc agtcggtgtc tttggtgcag ggcatcgatg catccactgc
    18961 gccaggttgc gccggaacgc agtaggtcgc gagcgtcgtc gctgaagatg aaacgagcac
    19021 ggtgcacact tcgccggcag agcagtcgga aaaattctgg catgtgcggg cgcccgacgg
    19081 cggcagacag cgacctgagt cgcacagcga ttgcagcgga cagggttctt cgagaaacac
    19141 ctggcgatcg tcggcgttgc gaccacagac catcgcaatg gcgcgatcac ggctgtcgca
    19201 gcgtctttcc ccaagcgtgc aggcgctcat gtcggggtaa tcgggattgg ccgtggtgca
    19261 gccgatggaa aagaaggcca acccaacggc aagacagaat gtgagcctca tcctcggcct
    19321 ccgtgagtgg aactagaatc gaccgacagc agccagtccg cttccgcttt cgaaatagaa
    19381 ggggagcaca gcgacttggc cttgcgagcg cttcagatcg cgccggtcga gatagtaaag
    19441 aacgccgaat gccgttgctg ctgccacgcc accaccgagc agtccaagcc ccgtcgccaa
    19501 gttgccttgg tatcgcgtcg ggcactggtt tgacgggcgg tccgtgcagg ttggctgacc
    19561 atcgcgcggt gacaggatca aaaacggtat cgacgtgatg agcaggccac cagccacgat
    19621 acccgatgcg atccagccga tccgataacc cgtgttgcgg gtgtgcggcg tcgctggttg
    19681 acttgtcgaa accgccggag ctgtggtcgt ctgcatgggg gaactgctgg gatctttgag
    19741 cgttccctcc ggtgacggtg ccgaggttgg cactggctgg gcaacaggtt ccgtcttcgc
    19801 aacttcaact ggtggtgctg cggcgggctt gagcttgggg agcagctcca tcgaggtctt
    19861 gcgcatcgac tcgaatgccc gtgtgaggtt gcagccttcg ccttcgtctc cacagcgacc
    19921 ggcgtagctt atttcgccct gctttcccgt cgggtcaatg gcggtgagcc gaatctgata
    19981 tgcgctgccg ccgaccgccg ttttcatggt gatttgcgca acgatgactc ggtcgagctg
    20041 aagctggctc gatgcttgtg cgatgcagcc gcctttttgg cagttcagcg cgtcggcact
    20101 gccagacaaa cgctgtcgga cttcttcgcc ggacagcacc gtaaagtcag cgcccg
//
LOCUS       DAORGN010000078        19885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1071_length_19885_cov_5.371256, whole genome shotgun sequence.
ACCESSION   DAORGN010000078 DAORGN010000000
VERSION     DAORGN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 19885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19885
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_19885_cov_5.371256"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKL17_10470"
     CDS             complement(<1..913)
                     /locus_tag="PKL17_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45199.1"
                     /translation="MNRRTFLDAASRAALIASWPAWLQKAFADESKAQTDDDGLTVVS
                     EGFRRAQRAGKPLLVLVIPKNSSDKYARGKIFGEYLNHATKTQHAPLQLCEVICATVQ
                     QLGQIVPSAGASDSLMILVETDKLPAEVRRINGKLPEGRSSFEISDREIEAEIPATGK
                     EKPEQSWDRRNRARQQIESREVKKRIDKFAALLADAVLPSPDVLGKRVVQQKAHAPAP
                     LVAAVQEKLQADRDATLSAEELELCAAQIAQYAQSLPSPRKDKLQEQLGSAAENKLLR
                     ARVPGSKWARSSGCGMRIEGEDQRRMVA"
     gene            complement(910..1470)
                     /locus_tag="PKL17_10475"
     CDS             complement(910..1470)
                     /locus_tag="PKL17_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45200.1"
                     /translation="MRILKRAPLLCVALLSACSEGFAIPEVERGLPDVPVAKPALRAI
                     GSWAYLWSPAVRAAILANSYGDRKAEARLPDLVASITESSVEVEPAALVGVYETDDQR
                     LLLNAAGGYSLLPRAGHTSSAASEHGTWMVKGTELLLHGSSQNIRQLSLLPGAELLRW
                     QEQVFQPATVRPPQPSNPDAFEGDDQ"
     gene            complement(1714..3252)
                     /locus_tag="PKL17_10480"
     CDS             complement(1714..3252)
                     /locus_tag="PKL17_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45201.1"
                     /translation="MSLRTNGRLSRREALSALGLLAAGSTSLAPFLTSCRQTQETPQS
                     REQIGTRIDPLGNPPKFLVVIAASGGASIVDSMLAVRESEAGANAIRLNAFPDTQVQS
                     VPGSPFRAVKFSSSKLGSISIPINTDQLAFVKKYADDMLVATSVGTSVNHAVAQKRAL
                     TGNNAWKGRTLQEIVALQFGQGMPLPNVNMAQGGYAERGIDDSLPAAMYAETVTNPAL
                     WPLGLDGTKGITGAPGKTVVDLARAARDELHSQLVFGQTYSDSVAMRRWLSQRAGSTT
                     LEAQDLITKLNVLPDLPPQVPLSQYGLASSPDGARVRQALPDYFNDPFQGQAALAFLL
                     IKYRVSAAVTIGPDFNVKLGGTSGLLNPPLAFDFSHNDHRSGQAFMWDRIMGTVDKLI
                     TLLKSEPFDTSGQSLWDRTLIYVATDFGRTRNRPDNAVQFGSGHDLNNGFLLLSPMVK
                     GNTILGGIDPATTLTYGFDARTGIAQPGKQQSNEADIFSGILTAMGADLSGSGLPDAS
                     AFKA"
     gene            complement(3263..3892)
                     /locus_tag="PKL17_10485"
     CDS             complement(3263..3892)
                     /locus_tag="PKL17_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45202.1"
                     /translation="MRHKSLLVGLALLSCTQTPSDPDVTELPTSPVAKSARGNIRFKG
                     PERINSDFSAILELRPESVCLELGLYACTSQVHLVALGGVDPYGAGVYESSGITASTT
                     PIIVDRIAWAACTQRVNSDLGNPDAAVIFRGIPLADQKLASPDGPEVHSAITQLVQRT
                     YLRNPYRPEVDRYVRLAKDIEAAGTATPAKDWMQSVCFAVLSSAESVFY"
     gene            complement(3882..5300)
                     /locus_tag="PKL17_10490"
     CDS             complement(3882..5300)
                     /locus_tag="PKL17_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45203.1"
                     /translation="MKKCTLEWSLVVTVLLLSGEAAAKPPAGGVFCQKYPSAPECVGK
                     QPSCTFCHVAPPQRNSFGTMVEKSLAVGMPRPLSDADFSTALPAALAAVESQDADGDG
                     TSNLDEIQGGTLPGDPSSKPGTNSCQGGENPQYSVCRYDLRHVYKKVLRDFCGVSPTF
                     ADVVAFVARPSDDDRRTFIDAEIERCTQTEFWRGKNGQLWKVAHPKIRPVGSLKEGED
                     KGGVPLADYYDDYNLFAYTQTDDHDAREVLTANYFVRRDVNPTRYSKVTTLASQKVDL
                     AHRAGNMTSAWTLSYFVMFTALPRNAASQMYRAYLGLDIAKQEGLYSVANEPQDYDAK
                     GVTTKECAACHATLDPLTYPFRNYNGIASAKYETQYIPNRLEQFFPNVSPRIAQTPEK
                     GAIFGKPVNNLLEWALTAVASDAFAISTTKDYWKLLVGHAPTPEENAEFVATWQRFQT
                     ANQYRVQKLLHDIVKTEAYGAP"
     gene            complement(5421..7205)
                     /locus_tag="PKL17_10495"
     CDS             complement(5421..7205)
                     /locus_tag="PKL17_10495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNK45204.1"
                     /translation="MSSPPLSFTRQSLDSSPNGPMPRWVRYGLWIVFLVYIAYSCFGI
                     AGPYLWGHFGYHGATYVLRARMTLRFHMLTPATWPGFVMPPLPQSFYFHHPIGYHHVL
                     TPFLLLLGDHDYVGHLVAALGGLVCLFALFQMVRRHVGDVAALIATAVYVALPIVCSF
                     SILIDAMLLAMACCILTMDSYLQYLKTPTTKWLVRGCLGLCLGGLLMWEAYFQAFFLG
                     LHCIVLFFMGRKLAGPIPRSTPVRWFLTTFVVSSLTMAFHFFLMWQKRAFDDFRASFH
                     SRSTATFAYAKAQHEKWLLLLYGKPILFLGGLWLVVFLIRLCVGKSRQRDFGVLLFFL
                     INTLYIFLFPAASSIHLYRVFWYSTFFALAISDLACDLYRLIQFSVDKWIPRDSQRHT
                     HLAIPAIAVTACLVGYFALVIPHSYRNLLESRVMMGTHGEPHYDPQYEKLRFAEEASR
                     LTEKTSCFVAYNLPHRLEFEYYLDRSFCTPQTWGNIGTLTQVEALHKQFPNMVIVMDS
                     QVEGAERAILLDLLRRHPGTLFGTYLHIDLGKQEKTLDAFRFVPHRPSLAWRYLVSHK
                     YPPLVPEKASSPILDRFLIELGRTSVRH"
     gene            7312..8361
                     /locus_tag="PKL17_10500"
     CDS             7312..8361
                     /locus_tag="PKL17_10500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK45205.1"
                     /translation="MPKQDAPDAPLQTENPVATDALDEPTGSLDLSVLVPLYQEQDNV
                     AMLVSRLFDVLPKLGLRFEVIAVDDGSRDKTLANLQAEAANHPELKVISLRRNYGQTA
                     AMMAGIDHASGECIITIDADLQNDPEDIPVLLEKMKEGYDVVSGWRQNRKDAAIRRTM
                     ISRVANRVISWASGVTLKDYGCTLKAYRAEIIKGVRLYGEMHRFIPIYASWMGARITE
                     IPVRHHPRRFGASKYGLERIGKVILDLLVVKFLERYFVKPIYVFGAFGLVSMVLSAIT
                     FSVLLYLRLVEHVSMILTPLPLLTVLFFLVGVMGILMGLLAEAMVRTYYESQGRPAYL
                     VRKRVNFGAAKVKST"
     gene            8361..10247
                     /gene="asnB"
                     /locus_tag="PKL17_10505"
     CDS             8361..10247
                     /gene="asnB"
                     /locus_tag="PKL17_10505"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNK45206.1"
                     /translation="MCGIAGFFGEGDRDDIERMTRSISHRGPDGDGYFCDDTLRLYLG
                     HRRLAIRDIAGGHQPMWNEDGSVCIVYNGELYNHESLRAELVACGHRFRSATSDTEVL
                     VHGFEEWGTELPQRLNGMFAFAIFDRNRQTLFLARDRFGEKPLYYRFDGKNLWFGSEL
                     RALLSHRQFCARVSLPSLQKFFAYGYFPTPHTLYEGVKKLPGGSWLSFDLRTATLREG
                     RYWQFRLQPDESLTEKDEPRLVEELQSLLTKAVAGRLVSDVPLGVFLSGGIDSSAALA
                     VMAKLRDPRGIHSFTIGFREPSFDESPFARQMAQAVGSDHHEQILGIDEVRQVLLPLL
                     GRLDEPLGDASILPTHLLSAFTRKHVTVALSGDGGDELFAGYDPFLALPFAGLYAALM
                     PRPGHAALRQLSSHLPISLGNMGIDFRIRRFLTGLSYPQSMWNPAWMSPVEPAWLKDL
                     FAKPLLPEDVYSEAIALWNRDPKLSVTDRSLEFFTTFYLQDDILHKVDRASMLTSLET
                     RAIFLDNGIVDFCQRLPSHFKIRRGQRKHLLKKALTGLLPKDILQRKKKGFGIPLAAW
                     LRQMDAPELPPKLRSIDGLRADFVAQRFFAHQKGQEDNRLLLWCWLSLLAVLSPHGDG
                     SSAS"
     gene            10284..11861
                     /locus_tag="PKL17_10510"
     CDS             10284..11861
                     /locus_tag="PKL17_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK45207.1"
                     /translation="MDQGVYELEAEIEEHHWWFIGRRRLFSTVIRSLGLSPSARILDM
                     GTSTGTNLRLLHDLGFSQVMGLDVSMLAARSCQEKQLGPVIVGSARDLPFADESFDLV
                     LATDILEHLDDDADGLREIARVLVPCGRVLLTVPAFQSLWSPHDEVAHHKRRYRVEAL
                     REKVEQAGLSVDDSYYFNFLLFPSIWLTRQAIRGLRLPFRNENKLNTPLLNRVFDAVF
                     SVDVKLAPQLRVPFGVSALCLSHKPKPSARPSPQPLHSERKQTIDDFGEQWTRYTDNE
                     GFYGSVELLKDIFGPLLSVDDLSGQRVAEIGSGTGRIVAMMLEAGAGHAVAVEPSSAI
                     VPMQNNLAKYGDRVTCLHIPGEELPSDLGLDYVFSIGVIHHIPDPRPVLRAMYESLRP
                     GGRCLIWLYGYEGNEPYLNMVLPLRRLTTKLPHAALSAVSHGLNLALDGYMLACRHVD
                     LPLKHYLDHVLSKFSREKRHLVIYDQLNPAWAHYYRRDEAEKLLRDAGFVDIKLHHRH
                     GYSWTVMGTRPHKDSTP"
     gene            11858..12793
                     /locus_tag="PKL17_10515"
     CDS             11858..12793
                     /locus_tag="PKL17_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK45208.1"
                     /translation="MTLRLASVSVDLDPLRCYYQIHGLGVCPETFSDVILARALPRFL
                     EVFDRFGIRSTLFVVGSDALDGMAGRPLLRKAAQLSHELGNHSFSHPYALSRLSPQDI
                     ESELAACEQVLDELRPSPSGPCGFRAPGYELSVPLLQSLLSAGYRYDSSVFPCPPYYL
                     LKLSVLGLLALRGQQSASIVGSPWQQLAPTQPYCPSPHNPNRSGDAKIVELPVAVTPA
                     LRLPAIGTFLLLSNPLRRVLLRGMTRQPFFNLELHGIDLIDAKLDGIPAELVAKQPDL
                     QLPLWQKMAALGEILRFLQDHAAVVPLSDAAQQLG"
     gene            complement(12790..14094)
                     /locus_tag="PKL17_10520"
     CDS             complement(12790..14094)
                     /locus_tag="PKL17_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK45209.1"
                     /translation="MTSEAPSNGRSLSLRDRLLLIRKEEIPPLLWSFFYFFFLLCSYY
                     VLRPVRDEMGIRAGVGNLPWLFSAVFGVMLLLTPIFGLLFARFSRRKLLPVVYLFFAS
                     NLGLFWLLFESGQYVHQATIAFFIWVSVFNLFAVSVFWSFMADLWNTDEARRLYGFIS
                     AGGTAGAIVGPALTALFSKRIGPMNLVLISLGCLLVCVTCIRQLREWAVRRVANDTPK
                     EGERPSILAGVRLALSSPYLLLICAYVVLYSTTSTFLYIQQAEIINKSIHVSAERTAL
                     FAQMDLAVNVLTLVMQIFVLQPLLSRLGLVVALTVLPILSLVGFLGLGLYPTLQMLIA
                     VVVLRRAGEFAIAKPSRETLFNVLGPVEKYQAKNFMDTVVYRGGDTASSWLLSWLKSV
                     GFGIVGVSLLAAPLAAVWAVIGALLGQLHKRLHEKTSAGEKS"
     gene            complement(14091..15215)
                     /locus_tag="PKL17_10525"
     CDS             complement(14091..15215)
                     /locus_tag="PKL17_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK45210.1"
                     /translation="MMKPAASALSRRDFLLATGSLTVAFASSAHGAAPPAAPKRLLVL
                     GGTGFIGPHIIDAAIRQGFQVTLFHRGKTNPTLFEGNPKVERILGDRNVGFDALAGKK
                     WDAVIDTSAYFPRQIEASTKALKASVKQYVFISSISVYKDTDKPMDESAAVGTIADPT
                     IEKITEGAYGPLKALCEQAATRGMDGRALNIRPGYIVGPRDTSDRFTYWLLRTQRGGD
                     MVVPGDPSDPIQVIDARDLADWIVRLVEKNVVGTFNAVGPAKPYTMQEVLSTCKQVTG
                     TDVKHTFLPTDYLRKNKLHEEFPIWSPPSGDSAYFHRVSNQKAVAQGLVFRPLLTTLT
                     DTLAWWKTLPPERQAKPRAGISADKEAEILRAYRASERKP"
     gene            complement(15249..15944)
                     /locus_tag="PKL17_10530"
     CDS             complement(15249..15944)
                     /locus_tag="PKL17_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45211.1"
                     /translation="MSKAVFESESKHAKPGRVLPISSYASANKHLHKLGPDGRLFLVT
                     VRPPKEALWLIAVLEQPTFSGTGWTAKPNPYRTTDITRIKDQLAFDTGKGIQAKPGAL
                     GMSLQTPRVLTATDAKLILDAALAQTVRVPAPRPVNLAVHEEGSALPCLCKRCLPAAP
                     ERIHHPSGEFVRAKVEIHERVLWFWVPVALTDDLESVVQAVAARLQNRIQPFPTPKSA
                     GERRDSEDAEDDE"
     gene            complement(16142..17479)
                     /gene="tig"
                     /locus_tag="PKL17_10535"
     CDS             complement(16142..17479)
                     /gene="tig"
                     /locus_tag="PKL17_10535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNK45212.1"
                     /translation="MQGSPVRFEFEDVSPVEKRLKVEIDKAQVNKKLDDSYRQLGRQV
                     NIKGFRPGKAPRSLLESMFGKKVASDAARELVNETIGDIIGKQTLRIISEPMLEELAD
                     AQKDQDLRYSARIELMPQIEVKDYEGIAVQKREGKVTDEQVNAALESRRQSQQELKPV
                     EGRDKAEAGDVLTIALTGTLSHLTYKDKEMHVDLGKPEQSPIPGLAAALIGVSLSATA
                     HSVDLTMPTEGLPKEVAGKTGHFKVAVKAAHVKHVPALDDDFAKDTGEADTLDGLKEK
                     IRTGLLEEDSEEARHEMRLSLVDELLKRNPVPLPQGLVARLARNFLENERGRMQLQMK
                     LLQDQRKEDPEAKFTMPTPEQLVQAAQAEAVRNLSIEFVMMALADKEKVEITDADVDT
                     HLGELAKERDKNVARVKAELQREDPGLQQLRAQLRLEKALDVLESKATITAKA"
     gene            complement(17658..18386)
                     /locus_tag="PKL17_10540"
     CDS             complement(17658..18386)
                     /locus_tag="PKL17_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNK45213.1"
                     /translation="MRDQLKLLEELQRHDAELLELEALCKTLPDRVQKVESAIAELDR
                     LLEEKRLQLQDTEAFRSEQEQEKKDAADQLSKAKAKSSAVRNLKEQNAVQRELETTRR
                     QIEQREEEIGKLTQAINEQKAKIAEHESRVGEQRKELAAEQDRVQKRIADMQAKLQAA
                     KAERDDLAKKLRPDVLKKYSAIRMKRGLAIVAVHDGTCRGCNMRIPPQLYNIIQRGAS
                     LEICPNCNRMIYWAKLLEEVAETA"
     gene            complement(18420..18493)
                     /locus_tag="PKL17_10545"
     tRNA            complement(18420..18493)
                     /locus_tag="PKL17_10545"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18457..18459),aa:Ile,seq:cat)
     gene            18669..>19885
                     /locus_tag="PKL17_10550"
     CDS             18669..>19885
                     /locus_tag="PKL17_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNK45214.1"
                     /translation="MTEPSIENPTNRSPQTDPLPGRFLDPSGRVLHFAVLSLLCLLYL
                     PFAGAYGLWDPWETHYGEVARQMASRNDWISLWWPGSPQDPASGVFFSKPVLTFWLMA
                     LSLKLFRLGHASTVFFDEMTVGWRAEWALRLPFLILGILGVWATYLLVSRVVSRRAAL
                     WASVVLATSTQWALITRQAMTDMAFVAPMTLATVYAALALLPQRRSVDDPDSPSLDHK
                     AFLPRRSVQFFGQELSWPHDRAFYGFLALFALFVVPQLVVNLIQLKSFSVSFRGHGFR
                     VAGPLAMMPFVLLFALSLLWCAQVQNRRSIYLWTAYALCGVATLAKGPAGIGMPVLTL
                     GLYLLLSGRLSELLGERRHVAPTGFLLKLPEPIRELWSYQGGLELTRGAAIFFCVAAP
                     WFVAMLARHGMPF"
BASE COUNT         3735 a         5817 c         5936 g         4397 t
ORIGIN      
        1 acgctaccat tctgcgctgg tcttcgcctt cgattcgcat tccacagccc gaagaacgtg
       61 cccacttgct acctggtacg cgcgcacgca gcagcttgtt ttctgccgca ctgccgagct
      121 gttcctgcaa cttgtctttc cgcggagacg gcagactttg ggcatactgt gcaatctgcg
      181 ccgcacagag ctccagctct tccgcgctca gcgtcgcgtc gcgatcggct tgtagttttt
      241 cctgaaccgc tgcgacgagc ggcgccggcg cgtgtgcttt ctgctggacg actctctttc
      301 ccaagacatc cgggcttggc aacaccgcat cggccaagag cgctgcgaac ttgtcgatcc
      361 gttttttgac ttctcggctt tcgatctgtt gacgcgcgcg gttgcgacga tcccaggatt
      421 gctccggttt ttctttgccg gtcgcaggaa tctctgcttc gatttcgcga tccgaaatct
      481 cgaaagagga gcgtccttct ggcagcttcc cgttgattcg tctgacttcc gctgggagtt
      541 tgtcggtttc gaccagaatc atcagactgt cgcttgcgcc cgcgctcggg acgatctggc
      601 cgagctgctg caccgtcgca cagataactt cacacagctg aagtggcgcg tgctgggtct
      661 tggttgcgtg gtttaggtat tcgccaaaga ttttcccgcg cgcgtatttg tctgagctgt
      721 ttttcggaat gaccagcacc agaagcggct ttccggcacg ctgggcgcga cggaatcctt
      781 cggacaccac agtcagtcca tcgtcatcgg tttgcgcttt gctttcatcg gcaaacgctt
      841 tttgtaacca cgcaggccac gaagcgatga gcgccgcacg actggcggca tccaagaacg
      901 tgcgacggtt cattgatcgt ctccttcgaa tgcgtccgga tttgacggct gcggcgggcg
      961 gactgtcgcc ggctggaaca cttgctcctg ccagcgcagc agctcggcac cgggcagaag
     1021 ggaaagctgt ctgatgtttt gacttgatcc gtgaagcaac agctcggttc ccttcaccat
     1081 ccaggtgccg tgctcgcttg cagcggatga cgtgtggcca gcgcgcggaa gcagcgaata
     1141 tccccccgcg gcgttcagca gcagtcgctg atcgtcggtt tcgtacacac cgaccagcgc
     1201 cgctggctcg acttctaccg atgattcggt gatcgaagcg accagatcag gaagtcgggc
     1261 ttccgctttg cgatcgccat aggaattggc caaaatcgca gcccgcaccg ccggactcca
     1321 cagataggcc caggaaccta tcgcgcgcag cgccggcttg gccacaggaa catcaggaag
     1381 tccgcgttcc acttcaggaa tcgcaaaacc ctcactacat gcagacaaaa gtgctacgca
     1441 gagcagagga gcgcgtttca aaatccgcat cagatcctcc gggcgaatgt catggtagat
     1501 gcgctgcacc tgtagacgcc tcgcgagcgc aaaagttctg ttctttgttt gcaccgggcg
     1561 tcttgccgcg cagatccacg aatttgtaga aagtgttccc caccacctat caaacagcgt
     1621 agcgctcaaa accctgccgc gcacgtacct tgaaacaagt tcctacttgt gcggtcttga
     1681 cttgcacttg ggggtttggg gatagacggt acgttaggct ttgaatgcgc tggcatccgg
     1741 caagccgctt ccggacagat cggctcccat tgcggtgagg attcctgaaa aaatgtcggc
     1801 ttcgttgctc tgctgtttgc cgggctgggc gattccagtg cgcgcatcga agccatacgt
     1861 cagtgtcgtt gctggatcga ttccgcccag aatggtgttt ccttttacca tcggtgacag
     1921 cagcaaaaat ccgttgttta gatcatgtcc gctgccaaac tgaaccgcgt tgtctggacg
     1981 attgcgggtg cgaccaaaat cggtcgcaac atagatcaga gtacggtccc acagactctg
     2041 tccggatgta tcaaacggtt ccgacttgag cagcgtgatc agcttgtcaa ccgttcccat
     2101 gatgcgatcc cacatgaacg cctgaccgct gcggtggtcg ttgtgactga aatcaaacgc
     2161 aagtggcgga ttgagcagac cgctggttcc tccgagcttc acgttgaaat ccggaccgat
     2221 cgtcaccgca gcagagacac ggtatttgat cagcagaaat gccagtgcgg cttgtccctg
     2281 aaatggatcg ttgaagtaat cgggcagggc ctgtcgaacc cgtgcaccgt cgggagaaga
     2341 cgccagacca tactgcgaaa gtggcacctg ggggggcaga tccggaagta cgtttagttt
     2401 ggtgatgaga tcctgcgctt ccagcgtagt acttcccgcg cgctgcgaaa gccatctgcg
     2461 catcgcaacc gaatcagagt atgtctggcc aaaaaccagc tgactgtgca gctcatcccg
     2521 cgccgctctg gccaggtcta cgacggtttt tcctggtgca ccggtgattc ctttggtccc
     2581 atcaagccca agtggccaca gcgccggatt ggtcacggtt tcggcataca tcgctgcggg
     2641 aagggaatcg tcaattccgc gctctgcgta gcctccctgg gccatgttga cattgggaag
     2701 cggcatcccc tgtccaaact gtagcgcgac gatttcctga agcgttcttc ccttccatgc
     2761 attgtttccg gtcagcgctc gcttctgcgc taccgcatgg ttgaccgacg ttcctaccga
     2821 tgtcgcgacc agcatgtcgt cagcgtattt ttttacgaac gcaagctggt ctgtgttgat
     2881 cggaatcgaa atgctgccca gcttggatga tgaaaacttc accgcgcgga acggacttcc
     2941 gggtacgctt tgaacttggg tgtctgggaa tgcatttaag cggatggcat ttgcgcccgc
     3001 ttcggattcc cgcactgcaa gcatgctgtc gacgatgctt gctcctcctg atgctgcgat
     3061 gaccaccaaa aatttgggcg gattcccaag cggatcgatg cgcgttccga tctgttcccg
     3121 tgactgcggt gtttcctgcg tttgccggca cgatgtcaaa aaaggcgcaa gcgacgtcga
     3181 tccggcggcc aaaagtccca gcgcagagag tgcttctcga cgcgatagtc tgccatttgt
     3241 gcgtagcgac atcttggctc ccttagtaaa agactgactc tgcggaagac agaacagcga
     3301 aacagaccga ctgcatccag tctttggctg gtgtggctgt tccggcggct tcgatgtctt
     3361 tggccagacg gacgtagcgg tccacttccg gacggtacgg attgcgcaga taggtgcgct
     3421 gcacaagctg cgtgatcgcg ctgtgaactt ccggaccatc cggacttgcg agcttctggt
     3481 ccgcaagcgg aatgccgcgg aaaatgactg ccgcatcggg attcccaaga tcagaattca
     3541 cacgttgggt acaggcagcc cacgcgattc tgtcgacaat gatcggcgtt gtgctggcgg
     3601 tgattcccga tgattcatac actcctgcgc catagggatc gacgccaccc agcgcaacca
     3661 agtgaacctg cgacgtgcaa gcatacagtc ccagttccag acagaccgat tccggacgca
     3721 gttccaagat ggcggaaaag tcggagttga tgcgctctgg acctttgaaa cgaatgtttc
     3781 cgcgcgctga ctttgcgacc ggcgaggtcg gaagttctgt gacatccgga tcgctcggtg
     3841 tttgggtaca tgaaagaagt gccagaccga cgagcaaaga cttatggcgc accgtaggcc
     3901 tccgttttca cgatgtcatg caggagcttt tgcacgcggt attggtttgc ggtttgaaag
     3961 cgttgccagg tcgccacaaa ctcggcattc tcttccggcg ttggtgcgtg tccgaccagc
     4021 agcttccagt agtctttcgt cgtcgagatc gcaaacgcat cgctggcgac ggcggtcagc
     4081 gcccattcca gcaggttgtt taccggcttg ccaaagatcg cgcctttttc cggagtctgc
     4141 gcaatccgcg gcgagacgtt tgggaaaaac tgctcgagcc gattgggaat gtactgcgtt
     4201 tcgtattttg ccgatgcgat tccgttgtag ttgcggaaag gataggtcag cggatcgagc
     4261 gtcgcatgac acgctgcaca ttccttggtg gtgactccct tggcatcgta gtcctgcggt
     4321 tcgttcgcga cgctgtacag cccctcctgt ttggcgatgt cgagtcccaa gtatgcgcga
     4381 tacatctgcg atgcagcgtt gcgcggaagg gctgtgaaca tcacaaaata gctgagcgtc
     4441 cacgccgaag tcatgttgcc ggcgcggtgt gccagatcga ctttctgtga tgccagtgtg
     4501 gtgactttgc tgtagcgcgt cggattaaca tctcgacgga caaagtagtt tgcggtcagt
     4561 acttcgcgcg cgtcgtgatc atccgtttgg gtgtacgcaa acaggttgta gtcgtcgtag
     4621 taatcggcaa gtgggacgcc tcccttgtct tcaccctctt tgagcgagcc gaccgggcga
     4681 attttgggat gcgcgacttt ccaaagctgt ccgtttttgc cgcgccagaa ctctgtctgc
     4741 gtgcagcgtt cgatttccgc atcaatgaat gtgcggcgat cgtcgtcgct gggtcgtgcc
     4801 acaaacgcca ctacgtctgc gaacgtgggg gatacaccac agaaatcgcg caggactttt
     4861 ttgtagacgt ggcgcaggtc gtaacggcac acgctgtact gtggattttc gccaccttgg
     4921 cagctgttgg tgccgggttt gctgctggga tcgccgggta gggtgccgcc ctggatttcg
     4981 tccagattcg atgttccatc accgtcggca tcttggcttt ccaccgcggc cagcgcagcg
     5041 ggcagtgcgg tcgaaaaatc cgcatccgaa agcgggcgcg gcattcccac agccaggctc
     5101 ttttccacca tcgtgccgaa gctgtttcgc tggggcggtg cgacgtggca gaacgtgcag
     5161 gatggttgtt ttcccacgca ctccggtgcg cttgggtatt tctgacagaa cactccgcct
     5221 gcggggggtt tggctgctgc ttcgccggaa agaagcaaga ccgtcaccac aagcgaccat
     5281 tccaacgtgc attttttcac gtactcaggg tagacgatac ggtctgtaat ggcgtagccg
     5341 cgctgtaagt gggccaaacg atggcgcttt gcgatgcgca aaaatctagg tgaagttccg
     5401 cgagcgcgat cgatggtttt ttagtgccgt actgaagtgc gtccaagctc gatcaggaat
     5461 cgatcgagga tcgggcttga tgccttttct gggaccagcg gcggatattt gtgggacacc
     5521 aaatatcgcc aagccagcga cgggcgatgt ggaacaaaac ggaatgcgtc cagcgttttt
     5581 tcctgtttcc ccagatcgat gtgcagatag gttccaaaca gcgtgcccgg atgtctgcgc
     5641 agaagatcca gcaggatggc gcgctctgca ccttcgactt ggctgtccat cacgatcacc
     5701 atgttcggaa actgcttgtg cagcgcttca acctgcgtaa gcgttccaat gtttccccac
     5761 gtctgtggcg tgcaaaaaga acgatccagg tagtattcga actccagccg gtgcggcagg
     5821 ttgtaggcga caaaacagct cgtcttttct gtcagccgcg acgcttcttc ggcgaagcgc
     5881 agcttttcat actgcggatc gtagtgcggc tcgccatggg ttcccatcat cacccggctt
     5941 tccagcaggt tgcgatagga atgcggaatc accagcgcaa aatagcccac aagacacgcg
     6001 gtgacagcga tcgcaggaat tgccaagtgg gtgtggcgtt gggagtctct gggaatccat
     6061 ttgtcgacgg aaaattgaat caatcgatac aaatcgcagg ccagatcgga aatcgccagc
     6121 gcaaagaatg tggaatacca aaacacccga tacagatgga tcgatgacgc ggcgggaaac
     6181 aggaaaatgt agagcgtgtt gatcaggaaa aacagcagta caccaaagtc tctctgccgt
     6241 gactttccga cgcacaagcg aatcaggaac acgaccagcc acagaccgcc cagaaacaga
     6301 atcggctttc cgtacaacag cagcagccac ttttcatgct gcgctttggc ataagcaaat
     6361 gtcgccgtgc tgcgcgaatg aaacgacgcg cgaaagtcat caaatgcgcg cttttgccac
     6421 atcaagaaaa agtgaaacgc catcgtcagg gaactgacca caaacgtcgt caaaaaccac
     6481 cgcacgggcg tcgagcgcgg aatcggtcca gcaagcttcc ttcccatgaa aaacagcacg
     6541 atgcagtgca gtcccaaaaa gaacgcctga aagtacgctt cccacataag gagtccgccc
     6601 aggcacagtc ccaaacagcc gcggacaagc cattttgtcg tcggcgtttt cagatactga
     6661 agataggaat ccatcgtcag gatgcagcac gccatcgcca gcagcatcgc gtctatcagg
     6721 atcgaaaacg aacagacaat cggcagcgcg acatacaccg ccgtcgcgat gagcgccgct
     6781 acatcgccaa catgacgccg caccatctgg aatagtgcaa acagacagac cagtcctccg
     6841 agcgccgcga ccaagtgccc aacatagtcg tggtcaccca gcagaagcag aaacggcgtc
     6901 agcacatggt gatagccaat cggatgatgg aaatagaaag actgcggcag cggcggcatg
     6961 acaaagccgg gccaggtcgc tggcgtcagc atgtggaatc gcagcgtcat cctcgcgcgc
     7021 aggacgtagg tcgcgccgtg atagccaaag tggccccaca gatacggtcc ggcgatgcca
     7081 aaacacgaat aggcgatgta gaccaaaaac acgatccaca gcccatagcg aacccagcgc
     7141 ggcatgggcc cattcggtga cgaatcgagg ctttggcgcg taaacgaaag gggcgggctc
     7201 gacatgatag acgatgcaga gtatcgcaga aggtggccaa ggcaagcgct tgcgctcggc
     7261 gctgcgacta cgctagcttg ctggctgatt cgtttttcgc cgggaacttg aatgcccaag
     7321 caagacgcac cagacgcacc tcttcagacg gaaaaccctg tcgcgaccga tgctttggac
     7381 gaaccgaccg gctcactcga cctgtcggtt ttggtgccgc tttatcagga acaggacaac
     7441 gtcgcgatgc tggtcagtcg gctgttcgat gtcctgccga agctgggcct gcgcttcgag
     7501 gtcattgctg tcgacgatgg cagccgcgac aagacgctcg cgaacttgca agccgaggcg
     7561 gcaaaccacc ccgagctgaa agtgatcagc ctgcggcgca actacggaca gaccgcggcg
     7621 atgatggccg gcatcgatca cgcatcgggt gagtgcatca tcaccatcga cgctgatctg
     7681 caaaacgatc ccgaagacat cccggtgctg ctcgaaaaaa tgaaagaggg ctacgacgtc
     7741 gtttcgggct ggcgccaaaa ccgcaaagac gccgcgatcc gtcgcacgat gataagccgc
     7801 gtcgccaacc gcgtgatctc gtgggcttcg ggtgtcaccc tcaaagacta cggctgcacg
     7861 ctcaaagcct atcgcgcaga aatcatcaaa ggcgtccgtc tgtacggcga aatgcatcgc
     7921 ttcattccca tctacgcttc gtggatgggc gcccgcatca ccgaaattcc cgtccgtcac
     7981 caccctcggc gctttggcgc atccaagtac ggtctcgaac gcatcggcaa ggtgatcctc
     8041 gaccttctcg tcgtgaagtt cctggaacgc tactttgtaa aaccgattta cgtgtttggc
     8101 gcttttggtt tggtgtcgat ggtgctgtcg gcgatcacgt tttcggtgct tttgtatctg
     8161 cggctggtcg agcacgtttc gatgatcctg acgccgctgc cgcttctgac ggtgctgttc
     8221 ttcctcgttg gtgtgatggg aatcctgatg ggattgctcg ccgaagcgat ggtgcggacc
     8281 tactacgaat cgcagggccg gcccgcttat ctggtgcgca agcgggtcaa ctttggcgca
     8341 gcaaaggtaa aatcgacctg atgtgtggca tcgccggttt ctttggcgag ggagatcgcg
     8401 atgacatcga gcggatgacg cgatcgattt cgcatcgcgg acccgatggc gacggctatt
     8461 tctgcgacga cacgctgcgg ctttatctgg gccatcggcg gctcgcgatt cgggacattg
     8521 ccggcggtca tcagccgatg tggaacgagg acggctcggt ctgcatcgtc tacaacggcg
     8581 agctatataa ccacgagtcg ctgcgcgccg agcttgtcgc ttgtggccac cgttttcgca
     8641 gtgcgacgag tgacaccgaa gtgctggtcc acggcttcga ggagtggggc accgagctgc
     8701 cgcagcggct caatgggatg tttgcgtttg cgatttttga ccgcaaccgg cagacgctgt
     8761 ttttggcccg cgaccgcttc ggggaaaagc cgctgtacta tcgcttcgac ggcaaaaacc
     8821 tctggtttgg cagcgagctg cgcgcacttt tgtcgcatcg gcagttttgc gctcgggtca
     8881 gcttgccgtc gctgcagaag ttttttgcct atggttattt tccgacgccg cacacgctgt
     8941 atgaaggcgt caagaagctg ccgggcggat cgtggctgtc tttcgatctg cggacggcaa
     9001 cgctgcgcga gggccgttac tggcagttcc gtttgcagcc cgacgaaagc ctgacggaaa
     9061 aagatgaacc gcggcttgtc gaggagcttc agtcgctgct gacaaaggcg gttgcgggcc
     9121 gactggtcag cgacgtgccg ctcggggtgt ttttgagcgg tggcattgat tccagtgcgg
     9181 cgctggcggt gatggccaag ctgcgtgatc cgcgcgggat tcactcgttt acgattggtt
     9241 ttcgcgagcc gtcgtttgat gaatcgccgt ttgctcgtca gatggcgcaa gcggtcggat
     9301 cggatcacca cgaacagatt ttgggcatcg acgaggttcg gcaagtgctg ttgccgctgc
     9361 tgggccggct cgacgaaccg ctcggggatg cgtcgatttt gccgacgcat ttgctgagcg
     9421 cgtttacccg aaaacacgtc acggtggcgc tgtcaggcga cggtggcgac gagctgtttg
     9481 ccggatacga tccgtttttg gcgctgccct ttgccgggct ttatgcggcg ctgatgccgc
     9541 gtccgggtca cgccgcgctg cgtcagctgt cgtcgcattt gccgatctcg cttggcaaca
     9601 tggggatcga ttttcggatt cggcgctttt tgaccgggct gtcctatccg cagtcgatgt
     9661 ggaatccggc ctggatgtca cccgtcgagc ccgcttggct caaagacctg tttgccaagc
     9721 cgcttttgcc cgaagacgtt tactcggaag cgattgcgct gtggaaccgt gatcccaagc
     9781 tttcagtcac cgaccgctcg ctcgagtttt tcacgacgtt ttatctgcaa gacgacatcc
     9841 tgcacaaagt cgatcgcgcg tcgatgctga catcgctgga aacccgcgcg attttcctcg
     9901 acaacgggat cgtcgatttt tgtcagcgcc tgccgagcca cttcaaaatc cgtcgcggac
     9961 agcgcaagca tttgctgaaa aaagcgctca ccggactgct gccgaaagac atcttgcagc
    10021 gcaaaaagaa gggctttggc attccgctgg ccgcgtggct gcgacagatg gatgcgccgg
    10081 aattgccgcc caagctgcgt tccatcgacg ggctgcgggc ggactttgtg gcgcagcgct
    10141 tttttgcgca tcaaaaaggc caagaagaca accggctgct tttgtggtgc tggctcagtc
    10201 tgctcgcggt gctttcgccg catggcgacg gatcatcggc atcgtgacgg gtgcttgcgc
    10261 gtaacctgcg aaggacaatt tctgtggatc agggagtcta cgagctagaa gcggagatcg
    10321 aagaacacca ctggtggttt atcggtcggc ggcggctgtt ttcgacggtg attcgctcgc
    10381 tgggactttc cccgtcggcg cgcattctcg atatgggaac cagcaccggc accaacctgc
    10441 ggcttttgca cgatctcggt ttttcgcagg tgatggggct cgacgtgtcg atgctcgcgg
    10501 cacgcagctg tcaggaaaaa cagctcggtc cggtgattgt cggcagcgcg cgggatctgc
    10561 cgtttgccga cgagagtttc gatttggtgc tcgcgaccga cattctcgaa cacctggacg
    10621 atgatgcgga cggactgcgg gaaattgcgc gggtgcttgt gccttgtggc cgcgtgctct
    10681 tgaccgtgcc ggcgtttcag tcgctgtgga gtccgcacga cgaggtggcc catcacaagc
    10741 gtcgctatcg cgtcgaagcg ctgcgcgaaa aagtcgagca agctgggctt tccgtcgacg
    10801 attcctatta ttttaacttc ctactgtttc cgtcgatatg gctgacgcgg caagcgatcc
    10861 gtgggctgcg actgccgttt cgcaatgaaa acaagctcaa cacgccgctt ctcaatcggg
    10921 tgtttgatgc ggtgttttcc gtcgatgtaa agctggcacc gcagcttcgc gtgccgtttg
    10981 gggtgtcggc gctgtgtctt tcgcacaagc ccaagccaag cgctcgcccg tcgccccagc
    11041 cgctgcacag cgaacgcaag cagacgatcg atgactttgg cgagcagtgg acccgctaca
    11101 ccgacaacga aggcttttac ggatcggtgg agctactaaa agacatcttt gggccgcttc
    11161 tgtccgtcga tgatttgtca ggccagcgcg tcgccgaaat tggcagtggc accgggcgga
    11221 ttgtcgcgat gatgctcgaa gctggggccg ggcatgccgt cgccgtcgag ccatcgtccg
    11281 cgattgtgcc gatgcaaaac aacctggcca aatacggcga ccgcgtcacc tgtctgcaca
    11341 tccccggcga ggagctgccg tcggacctag ggctcgacta tgtgttttcg atcggtgtca
    11401 tccaccacat tcccgatccc aggccggtgc tgcgcgcgat gtatgaatcg ctgcggcccg
    11461 gcggacgctg cctgatctgg ctgtacggct acgaaggcaa tgaaccgtat ctgaacatgg
    11521 tgctgccgct gcgacggctt acgaccaagc tgccgcacgc ggcgctgtcg gcggtgtcgc
    11581 atgggctgaa ccttgcgctc gacggttaca tgctggcgtg tcggcacgtt gatctgccgc
    11641 tgaaacacta tctggatcat gtgctgtcga agttcagtcg agaaaaacgg caccttgtga
    11701 tttacgacca gctaaacccc gcgtgggcgc actattaccg tcgcgacgaa gccgaaaagc
    11761 tgctgcgcga tgccggcttt gtcgacatca aactgcacca ccgccacggc tatagctgga
    11821 cggtgatggg aacccgcccg cacaaggaca gcacgccatg acgcttcggc tcgccagtgt
    11881 gtcggtcgat ctcgatccgc tgcgctgcta ctaccaaatc cacgggctcg gcgtgtgtcc
    11941 cgaaacgttt tccgacgtga tcttggcgcg ggcgctgcca cggtttcttg aagtgttcga
    12001 tcgctttggc attcgctcga cgctgtttgt ggtcggcagc gatgcgcttg atgggatggc
    12061 gggcaggccg ctgctgcgaa aggcggcgca gctttcgcat gaactcggca atcattcctt
    12121 ttcccatccc tatgcgctga gccggctgtc gccgcaggac atcgaatcgg agcttgccgc
    12181 ctgtgagcaa gttctcgacg agctgcggcc ttcgccaagc ggtccgtgtg gttttcgcgc
    12241 gcccggttat gagctgtctg tgccgcttct gcagtcgctg ctttctgccg gttatcgcta
    12301 cgactcgtcg gtgtttccgt gcccgccgta ctatctgctc aagctgtcgg tgctggggct
    12361 tttggcgctg cgcggacagc agtcggcatc gattgtgggt tcgccctggc agcagcttgc
    12421 gccgacccag ccgtattgtc cgtcgccgca caatccgaac cgatccggcg atgcaaaaat
    12481 tgtcgaactg cccgtcgctg tgaccccggc cttgcggctg ccggcgatcg ggacgtttct
    12541 gcttttgtca aacccgctgc gccgcgtgct cttgcgtggc atgacccgtc agccgttttt
    12601 taacttggag ctgcacggga ttgatctcat cgacgccaag ctggatggga ttcctgccga
    12661 gcttgtcgcc aagcagcccg acttgcagct tcctttgtgg cagaaaatgg cagcacttgg
    12721 cgaaattctg cggtttttgc aagatcacgc cgccgttgtg ccgctttctg atgccgcaca
    12781 gcagcttggt taggatttct cgccagcgct cgttttttcg tgaagtcgct tgtgcagctg
    12841 tccaagcagc gcgccaatca ccgcccagac cgctgcgagc ggtgccgcca aaagcgaaac
    12901 cccgacaatg ccgaaaccga cggacttgag ccacgacaga agccagctgc tcgcggtgtc
    12961 gccgccgcgg tagacgacgg tgtccatgaa gtttttggcc tgatactttt cgaccggccc
    13021 aagcacgttg aacagcgttt cgcgcgacgg cttggcgatc gcaaattccc cggcgcgacg
    13081 cagcaccacc accgcgatca gcatctgcag cgttggatac agtccaagcc caagaaaacc
    13141 aaccaggctc aaaatcggca gcaccgtcag tgccaccacc agcccaagcc gcgaaagaag
    13201 tggctggagc acaaaaatct gcatcaccag cgtcaggaca ttgaccgcca gatccatctg
    13261 cgcaaaaagc gccgtccgct ctgccgagac gtggatcgac ttgttgatga tctcggcctg
    13321 ctggatataa agaaacgtcg acgtcgtgct gtagagcacc acgtatgcgc agatcaggag
    13381 cagatacggc gacgacagcg ccagccgaac gcccgccagg atgctgggcc gctcaccttc
    13441 tttcggtgtg tcgttggcga cgcgacggac ggcccactcg cgtagctggc gaatgcaggt
    13501 cacgcagacg agcagacagc caagcgagat cagcaccaga ttcatcggcc cgatccgctt
    13561 cgaaaacagc gccgtcagtg ccggaccgac aatcgcgccc gccgtaccgc ccgccgaaat
    13621 gaagccgtac aagcggcgtg cttcgtcggt gttccacaga tcggccatga agctccagaa
    13681 caccgagacg gcgaacaggt tgaacacgct gacccaaatg aaaaacgcga tcgtcgcttg
    13741 atgaacgtac tgcccggact cgaacagaag ccaaaacagc cccaggttgc tggcgaaaaa
    13801 cagatagaca accggcagga gcttccgtcg agaaaaccgc gcaaaaagca ggccaaaaat
    13861 cggcgtcaaa agcagcatca ccccaaacac agccgaaaac agccacggca agttgccgac
    13921 gccggcgcga attcccatct cgtcgcggac aggccgcagc acgtagtagc tacacagcag
    13981 gaagaagaag tagaaaaacg accacaaaag cggcggaatt tcttccttcc gaatcagtag
    14041 cagacggtcg cgcaggctca gcgatcggcc attgcttggg gcttcgctcg tcatggtttg
    14101 cgttcgctcg ctcggtacgc gcgcaggatt tcggcttctt tgtccgccga gatccccgcc
    14161 cgtggcttgg cctgccgttc cggcggcagc gtcttccacc atgccagcgt atcggtcagc
    14221 gtcgtcagca gcggcctaaa cacaagtcct tgggccacgg ctttctgatt cgaaacgcga
    14281 tggaaatagg cgctgtcgcc gctcggtggg ctccagatgg gaaattcttc gtggagcttg
    14341 tttttgcgca gatagtccgt cggcaaaaac gtgtgcttga cgtcggtgcc ggtcacttgc
    14401 ttgcaggtcg aaagcacttc ctgcatcgtg tacggcttcg ctggtccgac ggcgttaaag
    14461 gttccgacga catttttttc gaccagccgc acgatccagt cggccaggtc gcgtgcgtcg
    14521 atgacttgga tggggtcgct cgggtcgccg ggaaccacca tgtcgccgcc gcgctgggtg
    14581 cgcagaagcc agtacgtgaa gcggtccgac gtatcgcgcg gtccgacgat gtagcccggt
    14641 cggatgttga gcgcgcgtcc gtccattccg cgcgtcgctg cttgctcgca cagcgccttg
    14701 agcggtccat acgcgccctc ggtgattttt tcgatcgtcg gatcggcgat cgtcccgacg
    14761 gcggcgctct catccatcgg cttgtcggtg tctttgtaga cggaaatcga cgaaatgaag
    14821 acgtactgct tgacggaggc tttcagcgct ttggtgctgg cctcgatttg acgagggaaa
    14881 tacgccgacg tgtcgatgac cgcatcccac tttttgccag ccagcgcgtc gaaaccgacg
    14941 ttgcggtcgc ccaggatgcg ctcgactttg gggtttccct cgaacagcgt cgggttggtc
    15001 ttgccgcgat ggaacagcgt gacctggaaa ccctggcgaa tggccgcgtc gataatgtgc
    15061 gggccgatga agccggttcc gccgagcacg agcagccgct tgggcgcagc aggcggcgct
    15121 gcaccatgcg ctgaacttgc aaaagcgacg gtgagcgaac cagtggcgag gagaaaatca
    15181 cgacgggaca gggcggaagc agcaggtttc atcatgcgac gcaccatagc agtggctgga
    15241 caagcgggct actcgtcgtc ctctgcgtcc tcgctgtcgc ggcgctcgcc tgcgctcttg
    15301 ggggtgggga agggctggat gcgattttga agtcgtgccg ccaccgcctg cacaaccgat
    15361 tcgagatcat cggtcagtgc gacgggaacc cagaaccaca gcacgcgctc atggatttcg
    15421 actttggcgc ggacaaattc gccgcttggg tgatggattc gctcgggggc agcgggcagg
    15481 cagcgtttgc acaggcaggg cagggcgctg ccttcttcat ggacggccaa gttcaccgga
    15541 cgcggcgccg gcacccggac cgtctgcgcg agcgctgcat cgagaatcag cttggcatcg
    15601 gtggcggtca gcacgcgcgg cgtctgcagc gacatcccga gtgcgccggg cttggcttgg
    15661 attcctttgc cggtgtcaaa cgcgagctgg tctttgatgc gggtgatgtc ggtggtccga
    15721 tacggattcg gtttggcagt ccagcctgtt ccgctgaagg tgggctgttc cagcaccgcg
    15781 atgagccaga gcgcttcttt gggtggacgg acggtgacca gaaacaggcg gccatcggga
    15841 ccgagcttgt gcaggtgctt gttcgcgctt gcgtagctgc tgatcggcag aacccgaccg
    15901 ggcttggcgt gcttggattc gctctcgaag acggccttgc tcacgatggc cagcatgtcc
    15961 ggcataaaaa catcgtaccg gaacgctcag attgtttggc agcttacaga tggaagagat
    16021 gtcaccgtgg ggcccccctt ccatcggacg cttttttccg tgcggctctc gcagcccaga
    16081 aaaaaggtcc tcagtcggcc ccactggatg cgtcggagat tggcattccg acggaggggt
    16141 attaggcttt ggcggtaatg gtggccttcg actcgaggac gtcgagggct ttttcgagcc
    16201 ggagctgcgc acggagctgc tgcaggcccg gatcttcgcg ctggagctcg gctttgaccc
    16261 gcgccacatt cttgtcgcgc tctttggcca gctcgcccag gtgcgtgtcg acgtcggcgt
    16321 cggtgatctc caccttttcc ttgtcggcca gcgccatcat cacaaactcg atcgacaggt
    16381 tgcgcacggc ttccgcctgc gctgcctgca cgagctgctc tggcgtcggc atcgtgaact
    16441 tggcttcggg atcttccttg cgctgatctt gcagaagctt catctgaagc tgcatccgac
    16501 cgcgctcgtt ttccaggaag ttccgcgcga gccgcgcgac cagaccctgg ggcagcggca
    16561 ccgggttgcg cttgagcagc tcatcgacga gcgagagccg catttcgtgg cgcgcttcct
    16621 cgctgtcttc ttcgagcagg ccggtgcgga ttttttcctt cagcccgtcg agcgtgtcgg
    16681 cttcgcccgt gtccttcgcg aagtcgtcat cgagcgccgg cacatgcttt acgtgcgcgg
    16741 ctttcacggc gaccttgaag tgacccgtct tgcccgcgac ttccttgggc agaccttcgg
    16801 tcggcatcgt cagatcaacc gaatgcgccg tcgccgacag cgaaacgcca atcagcgccg
    16861 cggccagccc cggaatcggc gactgctcgg gcttgcccag atcgacgtgc atttctttgt
    16921 ctttgtacgt caggtgcgac agcgtgccgg tcagggcaat cgtcagcacg tcaccggctt
    16981 cggccttgtc acggccttcg accggcttca gctcttgctg cgactggcga cggctttcga
    17041 gcgcggcgtt cacctgttcg tcggtgactt tgccttcgcg cttctgtacc gcgatgcctt
    17101 cgtagtcttt gacctcgatc tgcggcatca gttcgatgcg ggcgctgtag cgcaggtcct
    17161 ggtctttttg cgcgtcggcg agttcttcaa gcatgggctc gctgatgatt cgcagcgtct
    17221 gcttaccgat gatgtcgccg atcgtctcgt tgaccagctc gcgggcagcg tccgaagcca
    17281 cctttttgcc gaacatgctt tccagaagcg agcgcggcgc tttgccggga cggaagccct
    17341 tgatgttgac ctgtcgtcca agctgacgat agctgtcatc gagcttcttg ttgacctgcg
    17401 ccttgtcgat ctcgaccttc aagcgctttt cgaccgggga cacatcttca aactcgaacc
    17461 gtacggggga gccctgcatg aatcgcctca cttaccttcg tcgtaggctg cggttttggg
    17521 ccgcttgcct tttggccacc ttgtcggggc gctgtctaat ttgaactgtt gagagcgcaa
    17581 gttcccatgg caactgccac ttttgcgcaa acgttcaggc agtgtagaga catcacctgc
    17641 ctgtcgtaaa gccctggcta cgcggtttcg gcgacttctt cgagcagctt ggcccaatag
    17701 atcatccggt tgcagtttgg gcagatttcc agagacgcgc cgcgctggat gatgttgtaa
    17761 agctgcggcg gaatccgcat gttgcagcct cggcaggtgc cgtcatgaac cgcgacgatc
    17821 gccagcccgc gcttcatccg aatcgccgag tatttcttca gcacatcggg ccgcagtttt
    17881 ttcgccagat cgtcgcgctc ggccttggcc gcttgcagct tggcctgcat gtccgcgatc
    17941 cgcttttgca cccgatcctg ctcggcggcc agctctttgc gctgttcacc aacccgcgat
    18001 tcgtgctcgg cgatcttcgc tttttgctcg tttatcgctt gggtcagctt gccgatttct
    18061 tcttcgcgtt gctcaatctg ccgtcgcgtc gtctccagct cgcgctggac cgcgttctgc
    18121 tctttgaggt tgcgcaccgc cgagctcttg gctttcgcct tgctgagctg atcggcagcg
    18181 tctttctttt cctgctcttg ctcgctccgg aacgcctcgg tgtcttgcag ctgcagccgc
    18241 ttttcttcca aaagccgatc cagctcggcg atcgccgatt cgaccttctg cacccgatct
    18301 ggcagcgtct tgcagagagc ctctagttcc aaaagttctg cgtcgtggcg ttgtagctct
    18361 tcgagcagct tgagttgatc gcgcacgctc ttcctccgaa aagttgaaaa acgaaaacgt
    18421 gggcccacct ggacttgaac cagggaccag ccggttatga gccggccgct ctgaccaact
    18481 gagctatggg cccgagaagc ggcgcaaacc gtagcgaagg attcagcagt tggcaagcaa
    18541 gcaggtcgtg ctgtgacgaa acaagttttt cgggaagcct tgttcgcaat ccctcttgat
    18601 tggtccgggc cggttattgc atggtcacgc cgcgtgcgcg acctggggac ggtccgccga
    18661 atcctcgaat gacggaacct tccatcgaaa acccgacgaa ccgatcgccg cagaccgatc
    18721 cgctgccagg tcgttttttg gacccaagcg ggcgcgtgct gcacttcgcg gtgctgtcgc
    18781 ttttgtgttt gctctatctg ccgtttgccg gcgcttacgg gctgtgggac ccgtgggaaa
    18841 cccactacgg cgaagtcgcc cgccaaatgg cgtcgcgaaa tgactggatt tcgctgtggt
    18901 ggcccggctc gccgcaagac cccgcgagtg gcgtcttttt ttccaagccg gttctgacgt
    18961 tttggctgat ggcgctgtcg ctcaagctgt ttcgcttggg ccacgcgagc acggtgtttt
    19021 tcgacgagat gaccgttggc tggcgggcgg aatgggcgct gcgtctgccg tttttgattc
    19081 tcggcatctt gggcgtctgg gcgacgtatc ttttggtgtc gcgggtggtg tcgcggcggg
    19141 ccgcgctgtg ggcttccgtg gtgctggcga cttcgacgca gtgggcgctg atcacccgcc
    19201 aggcgatgac cgacatggca ttcgttgcgc cgatgacgct cgcgaccgtt tacgcggcgc
    19261 tggcgcttct gccgcagcgt cgttctgtcg atgatcccga ttccccgtct ttggaccaca
    19321 aggccttcct accgcgtcgc agcgtccagt ttttcggcca agagctgtca tggccgcacg
    19381 atcgcgcttt ttacggcttc cttgcgctgt ttgcgctgtt tgtcgtgccg cagcttgtcg
    19441 tcaatctgat ccagctcaag agcttttccg taagcttccg cgggcacggt ttccgcgtcg
    19501 ccgggccgct tgcgatgatg ccgtttgttt tgctgtttgc gctgtcgctt ttgtggtgcg
    19561 cgcaggtgca aaaccggcgg tcgatctatc tgtggacggc gtatgcgctg tgcggtgtgg
    19621 cgacgctggc caaaggtccg gctggcatcg gcatgccggt gctgacgctt gggctgtatc
    19681 tgcttttgtc ggggcggctc agcgagctac tcggcgagcg gcggcatgtt gctccgacgg
    19741 gatttctgct caagctgccc gaaccgattc gcgagctgtg gagctaccaa gggggtctgg
    19801 agctgacccg cggcgcggcg attttctttt gcgtcgcagc cccgtggttc gtcgccatgc
    19861 tggcccgcca tggcatgccg ttttg
//
LOCUS       DAORGN010000079        19611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1092_length_19611_cov_6.409388, whole genome shotgun sequence.
ACCESSION   DAORGN010000079 DAORGN010000000
VERSION     DAORGN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 19611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19611
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_19611_cov_6.409388"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..788)
                     /locus_tag="PKL17_10555"
     CDS             complement(36..788)
                     /locus_tag="PKL17_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45215.1"
                     /translation="MASLVFVGDAGDVEARRKSSRVRRADECKAPPVVSPQEQDKALG
                     HYDSALTHWESKEWDRARSDFQNAYNITKLPDLLINLALVSEKQGKLNDSIGYLEAYI
                     ENCPNAPDLDKARQKIDELKIAIAIEAGKNPTRTVKASLPPTPAIALMATGAGLLIMG
                     IGLGSAALVNSHEVSSPDNRTKWWTQDLQALETAGQRMATAAYTFDAIGVAALTAGVI
                     WTGVFYAQGNTGLSLAIGPKSGGLGAVVLGRF"
     gene            1086..1754
                     /locus_tag="PKL17_10560"
     CDS             1086..1754
                     /locus_tag="PKL17_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45216.1"
                     /translation="MKALHFAAMAFALLAGCGPWSTSSHTCVGHSCGSTWSGGGGLPT
                     TQYSTFIIDAGAAIQLPQTTLGITTNGTGDWVLAWQGDTYAHQFHGSITCPTSCQIAY
                     ARFDNAYPGDSAQITGTNQIVFQGVTNAAIPQNLLFRMTQQPVTFDLYIDNQPAIGEA
                     VFASQGALSTTDTMPFSLVSSNVAYEGKTDRAPSFVAKPPQDGVERVTTVAAPPASSQ
                     SVDK"
     gene            complement(1708..3216)
                     /locus_tag="PKL17_10565"
     CDS             complement(1708..3216)
                     /locus_tag="PKL17_10565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK45217.1"
                     /translation="MVTAPRPGPDPLPPSASSAELLTPEFRLRRFQNWFFLGLMYGFF
                     YMSRYNLGAVQSAVAEQFGWSHDDYGTITSAGLATYGCAVFLNGPLADRIGGKRAILI
                     GAAGAAFFNLLFGLCHLLIVKPAVMAGGQVISQAQLGAGLNSTSVIAMFAVLWACNHY
                     FQSFGALSIVKINAAWFRVTERGKFAGIFGIMIQLGRTLAMVLLPALLLIVPWQYAFF
                     VPALLLTVIWGFCYFLIQNTPALAGHGEFDTGDESPDEARTDASLGFVLRKVFTRKAP
                     WIIALASFCIGSVRHVVDGWYPRYISSMFDVGPRELFSFAPYQMASVAMPFAAVVGGL
                     VAGNASDRLFQSRRAPVIFFAFLGMAVCMLSLRGTIYNPWGAAVVLIAVSFFVQSAHS
                     LVGGAASMDFGGRKAVATAAGLFDGAQYLGGALAGKAVGKLLLRYKAPGLHGHEFEIW
                     PYVPLIFAVLGALIISRLWNALPGRGGHGAPIAKSGGPTYPLTASLPAEQQR"
     gene            3349..4797
                     /locus_tag="PKL17_10570"
     CDS             3349..4797
                     /locus_tag="PKL17_10570"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK45218.1"
                     /translation="MLSNANSELPWEQQALQLVIETVQEALHQKSPAEQQSLQMALMT
                     LRGFLAASRLTQEDGGTTWEPYLEPISPPDMSRIGAWSQTLKERREAASLSRHKLAEL
                     SGIPEGALEALESGTYAPSQMSLLHLLSVPELDLETQSLPWEASASDPNLALNCWLAP
                     GFDPVKMIKDLALQVNGHGGHIEQSYLYMDHMSAAHWCAIAEQKDYDAMQSSMPLEKT
                     AARILRTNAQCGLDVVALGPGSARNEVRLVKQLMTQSGHADLRLYLLDISQPLLSVAY
                     RHAAEVLADNGGAVFAIQGNFHHLPRYGQLLYSPRRAHRRRIITMLGHTFSNLENELR
                     FMRNSLCGFATGDLLVMDFRLAYTSSCDPDEIYRKDPTFASRRSTDLSQRRDKFLAGP
                     FRRYGADQVDVEISPALDLSCAVPGSYGVDLRARVRGDGADREFSVYYSRRYEPNQLL
                     HALRREGWETVESWEYGGDSSPCLLVLLRKLS"
     gene            4866..5792
                     /locus_tag="PKL17_10575"
     CDS             4866..5792
                     /locus_tag="PKL17_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNK45219.1"
                     /translation="MRSLLLIFMAMLVGCGDAEVGQLPTEAPMLSLPNKLGTLSAQLS
                     PQTAGPLDGRPYQYKVPSRYDKTRPTPLVVMLHGFSASGSVEELYLNISAIAESKTFL
                     YAFPDGTQNPVGLRFWNAMEWCCNFFKSGVDDVAYVNAVISDMQSRYNVDPKRIFVVG
                     HSNGGFMAHRVACELSDKVAGIVSLAGQQWNDPTRCTPKQHVPILHVQGTLDAVIGYL
                     GMPGLYPGAKETVQIWAQKNGCGGRLTYSGENRDLDTVLLGAETTVEKFTGCPSDGPV
                     ELWTIHGGGHVPVFNSFFTSSFYDFLMSHPKP"
     gene            complement(5814..6947)
                     /locus_tag="PKL17_10580"
     CDS             complement(5814..6947)
                     /locus_tag="PKL17_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNK45220.1"
                     /translation="MSAHNRSVSRSLFLAPLAIGTLALSGCPQQEAAKPAEAPAAPAP
                     PAAAPADPPKSPTIDPARLAVFGKPLPASYDSTDNPPTDEKTALGRQLYYDKRLSKNQ
                     DLSCNSCHDLAKYGVDGAKFSSGHKKQLGGRNAPTVYNAAGHVAQFWDGRAKDVEEQA
                     GGPMINPVEMALPDHKRAETVLQSIPGYVEAFKKAFPGDKQPVTIKNATAAIGAFERR
                     LLTPSRFDKFLGGEVNALNDVEKAGLLKFLDVGCVACHSGTLLGGTAYQKLGAVKPWP
                     NQADLGRAALTKNDADKFLFKVPSLRNVEKTAPYFHDGSADKLADAIKKMAAHQLGRD
                     LSDADAQSIAEFLGSLTGDIPKEYIAEPQLPPSGKTTPKPDPA"
     gene            7108..11235
                     /locus_tag="PKL17_10585"
     CDS             7108..11235
                     /locus_tag="PKL17_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45221.1"
                     /translation="MQELPASGEGQLSVCPYCGGSIVEGDAHDCEEMGSGDTMSVAAP
                     VKIGTAPVTSGSAGGAQTGKSTAPLAVAVGKGAVLVTGGGIAIGGSPGAASSPAKSDP
                     QKRASSAAREKLSASDAKPPAKSPVGSSTDELYGLIIDGRYEVLEKASQGGMGTVYKA
                     RHATLRNLLAVKILRKPKDEVSRRRFLQEAQLLSQIKHPNIVQVIDFGELPDGKSYLA
                     MEFLSGPTVSAAVREGQMTPLRACRIGAQIARGMHHVHEQSIVHRDLKPDNILLVNQD
                     GNVDFVKIIDFGIAKDVKAVPSVQVSGPMRVSSASLALKSQPQPPAEPEPEPDEDMSL
                     DDTPGQNLTQAGQLVGTPLYMAPEQIRSGLHDAKGDQYSLGCILYFMLTGQPVFAGKR
                     ADDIMSAHVKQKVVPPRQRVPGLVISGELEAVVLRALAKDPDERFPSMRALESALEQC
                     IEQIAPSLPSVRLIGQKAKRFAPLWVPVVVGLLAFFVYLWRSQRGRSDEVSQEELLAV
                     RARALSELNKQSGSTDLRLRLPALRALGQSRDGQQRALLEKLLDDSDPAVVAQAAESL
                     GLLGEPAAVQTLLIHVGRASDPATKTALAVALERLGEPKGTQLLYEGLAGKDTELRLL
                     SALLLCDRGNRTAIELLLSVLEKGQVGGPIELSVLGCLARSGNDASYKALLVRLQRSE
                     PREAQLKAAQQLIPLGEARAQVLLDKLAAEPGRDQLLAARYLASTEHPETAQVFRAVI
                     GDANASSTATILAAEGLGLSGRLQDVRLLDRQLRGAGDEQIKLAAAEAIVRLAATDVR
                     LMSQRSLLWARDALTSGSWAIRQAGVATLSDRQEQEVGPLIVSMLRDPVSQVRKAAVR
                     AISRRNDAGMLDVLKAQLSDGDSTVRLESLQAIVRLAKRLLDGGQRPAVGQVSDWLRE
                     VVQKGNQSEQVVARSGLLLLGDSSQKPALKELAASAEEENRKLLAEQPGVDSEILTGM
                     LTDRSDAVRFVAARRLAERGNASAKPVLQETLKKGTTESVTASVLLNRLGEKTQQPTQ
                     VATSVPVENRMLLVESVQSLDASIALPLLLQAARDPEPLVRRLVVEVAADLPGTTTES
                     TPGLPVLKLLSTDSDAAVRARVWGLLSRLQSDPHATKPAAKDRVDSSASKKPDPSSGN
                     PTVNPTSNPTEQPASGQGKLAIVAAPGTLVQIDDKPWQNATSAGFVLPAGKHTLRSLA
                     GERTIEIVSSQTLSLTLPESESEQQLAKGIDLLGKSDYRRAQKLFDKAHGQCKKKKAL
                     ATPCELLAFEGGYHLATALESGHRLTEAMTQYQKLLKSTPRSKGRFDRRSEIQAAVSR
                     LSSQMGEVVIASRKKGKCQQSSLWVLPGTHIISVAGKSQQVQVSAQQRLSVGSCE"
     gene            11232..12818
                     /locus_tag="PKL17_10590"
     CDS             11232..12818
                     /locus_tag="PKL17_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK45222.1"
                     /translation="MSHPLRWLLAVLLLWRCAEVQALASPDSESSRQGRRHRRSERKH
                     ATKTTADHQAGTDSTEDRAGDVRSRPKVTALLDEARSALLAQDPDGALKKAESAYKKQ
                     ASSETLCLLAQIAVAQKRFVDAHDLFRRCLADTVVMVDPAVRSEAQQIPGAALNGLGD
                     LHVSGDKDALVYVDDRLRGSLPLLLPVRVAPGLHAVSLSSSGHWTRGKVEVPAGQARE
                     IRFDALSGAVLVSVPPMVVFAQESGTSLEPQLLSALEKGSQLVGYSLTLRDGKAEVCD
                     GLLPECLLDAARRTSAGYVLAIRVSLSAAGKEIFLSLWDAQVGELAGQETARCAPCSD
                     EAQLATVAERLAAALKRGRSRPRGALTVSSIPSAAQVKLGGRLLGQTPWKGSVFAGQQ
                     QIEVAFPGYEPQTLITTVAPGQQASLSALLSPLLLAEPGPRGSVVVSPALRRPMWRVV
                     TGAAAMGVGMLMIGIGGSGLAADGTCVPPLQPPAQVCRETIHSATAGGALLGVGLSLT
                     IGGAVLVGLPPRPEAAPSGP"
     gene            12902..13318
                     /locus_tag="PKL17_10595"
     CDS             12902..13318
                     /locus_tag="PKL17_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45223.1"
                     /translation="MSEDLVVLREILQRELETINAYEKMLARISDPALREIIDHVTDE
                     EREHVAEMYHLIMDRDPKQRERSAQSHAQLVSMSLLQTAAETPAGHEPNAAQAAGLSD
                     KLIAPASPARRDPMPPPPPIFPDGGWSVGGLKRSAP"
     gene            13407..14264
                     /locus_tag="PKL17_10600"
     CDS             13407..14264
                     /locus_tag="PKL17_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNK45224.1"
                     /translation="MPDESNFLTHEHLPLSEARWRELNMSVVDVARRKLVGRRFLDLY
                     GPLGAGVQTVHNDVYAGTERAAIGILGEEPIAPVRAAGRQILSIPILYKDFSLYWRDL
                     QTTEQFGMPLDLSAAAGAASFVADREDDMIFNGIADLNLPGLTTVPGRTVMPLGDWNQ
                     VGAALQSVTVAIERLVETGFYGPYCLVTSPFLYAKLARFVENTGVSELGHIRELCTDG
                     VFRSSVLPRDRAVLLSTGPQNFDLAVAQDFRVAYLGAEQMNHPFRVFECAVLRIKRAQ
                     AICTLEAKA"
     gene            14299..14595
                     /locus_tag="PKL17_10605"
     CDS             14299..14595
                     /locus_tag="PKL17_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45225.1"
                     /translation="MLVSEQEEWPSWLVPMSAMDEDEDEDFEDEDFDDEDFDDEDSDE
                     DFDEDDADDEEFDEDDDEDFDEDDDEEAEDDDEDDDDYFDEDDEDEEDFDEDDE"
     gene            14663..15190
                     /locus_tag="PKL17_10610"
     CDS             14663..15190
                     /locus_tag="PKL17_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNK45226.1"
                     /translation="MKAEDWNVTVGSGKDQRKLQVKIESHEGDVYRVRIGDETTTVRA
                     VRIENGSYSLIDEMGRQRGIDFDGMLPEAKLSIDGGDAISVVVQDAQSLLDAAAADAS
                     VGEIRASMPGKVVKVLCKVGDVVKSGQALLIIEAMKMENELRATGSGKVESISVREGQ
                     TVEGGQSLLVLGGVS"
     gene            15187..15744
                     /locus_tag="PKL17_10615"
     CDS             15187..15744
                     /locus_tag="PKL17_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45227.1"
                     /translation="MSIRRWFSLLLVVVGVAGAGCGKDDGFVRDDGQAAFEGRNIKFQ
                     STYVYFPSDFSLSIVRDTMPQDEVCAILKRYSSTTPKLPGLPVLPYKDEHYALVVTVD
                     SVQPGDDVSFDVTDTGGAGDMRYAALAHYGVGKNRDSDWALRSGGYVRITELSHYKHV
                     AGKLALRFPSGEKLDEDFSIDVCPP"
     gene            15894..16874
                     /locus_tag="PKL17_10620"
     CDS             15894..16874
                     /locus_tag="PKL17_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK45228.1"
                     /translation="MTTHAQGKTMIHVDGLTKHYGKVKAVNNISFDVHAGEVLGFLGP
                     NGAGKTTTMKILTSYLSPTSGRAVVAGCDVFDQALEARARIGYLPEGTPLYRDMTVLE
                     YLNFIADVRNIPSGSKRTRRLRDVVEQCGLGDRLGFLIGELSKGLKQRVGLAQAILHE
                     PEVLILDEPTSGLDPNQIVEIRQVIKQIGEKKTVIFSTHIMQEVQATCSRMLIISKGK
                     IAADGKPDELQRKAQGQMSLQVELLGDENQAKSGLESITGVESVRALPGEESELPGGH
                     RFVVMAKSGSDPRAEIFKMATAKGLQLTELHRERASLEDVFRNLTAGSAR"
     gene            16898..17614
                     /locus_tag="PKL17_10625"
     CDS             16898..17614
                     /locus_tag="PKL17_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNK45229.1"
                     /translation="MRNMTTIMRRELGAYFNSPIAYVVISVFLVLSAFLFFNALFLQQ
                     QADLRELFDGFWTTLLVAILGPAITMRLFAEERSNDTLELLLTMPVTDWDVVLGKYLA
                     ALVLFGYAMLAMLCFAVSVSLLGPLDKGAAFAGFAGLYLTTATYMAIGLMASAFTKNQ
                     IVAFLLAVTLCLLLWLVGKVVQVMPPAIQPFVDWIGVDGHIGNMARGVFDTRDLLYFL
                     TIIVSCLFVTQTTLESRRWS"
     gene            17617..19302
                     /locus_tag="PKL17_10630"
     CDS             17617..19302
                     /locus_tag="PKL17_10630"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNK45230.1"
                     /translation="MSKPLHKSRTAFNAALFVFLSIGALGFANVISLKHFGRIDLTKD
                     KIYTLAGASRDLVAKLPDRMNVKLFMSEDLKPPFKQHAQFARDLLAEYATYSQGKFNL
                     EVIKIGEGDEARKKEEEATKCGVQKSNRGVVSANKFEIGATFLGVCFAYGDKTEAIPV
                     IDRDEGLEFMISGLIKQMTVKKKKIGFTTGQGELGSGHGGGLQLLSEQLKASGYETIN
                     VELKAMVSSDIDLLLVIGAKQPFSERAKYVLDQFLMSGKSVAFLIDGQQMQTPRGQMA
                     PGMEMPQIAQNNDVGLDDLLGAYGVKIKQDMVLDKRNFPGLVNVGQQMYLMNHPVFLK
                     VTPLPQVHAVNENMPGALLSYASSVELVGDAKDGKGPVQFVKILSSSNQSWRPSGPFV
                     FMPGPNQTPPTPSEDRGPFTLGVIGQGKFKSAFAGKPIVKEDGTKIPADQSQPGVEPM
                     KSEAQNGARLVVIGDSDFIGDQFIGTGARAGLRDYLANLVYTMNIIDWLAQDEALAAV
                     RKKGMQSRPLDQVSESTVTLVKLVNIGALPGLILLIGLIRFRIRQSARQSAKL"
     gene            19320..>19611
                     /locus_tag="PKL17_10635"
     CDS             19320..>19611
                     /locus_tag="PKL17_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45231.1"
                     /translation="MEKKNLYALVAFVALAVSAVYSLRSPDKGERVGERPRAIAEIKA
                     GSIATVEVAQPGDKDKVTLTKKGDKWQVTAPYDKPADQTAAKSAAEALEKVRW"
BASE COUNT         3949 a         5614 c         6307 g         3741 t
ORIGIN      
        1 tgcggcaacc gtgcggggca aaatcggtct tctcatcaga acctcccaag gacgacggcg
       61 ccgagaccgc ccgacttggg tccgatcgcc agcgacaagc cggtattgcc ttgcgcataa
      121 aaaacgccgg tccagatcac gcccgccgtc agggccgcca ccccgatggc gtcgaatgta
      181 taagccgccg tcgccatccg ctgtcccgct gtctccagcg cttgcaggtc ttgcgtccac
      241 cacttggtgc ggttgtccgg cgagctgact tcgtgggaat tgacgagcgc ggcactgccg
      301 agaccgattc ccatgatcaa aagaccggcg ccggtggcca tcagcgcaat cgccggcgtc
      361 ggtggcagtg aggctttgac cgtgcgggtc ggatttttgc cggcttcaat cgcaattgcg
      421 atcttaagtt catcaatttt ttgccgggct ttgtcgagat cgggggcgtt cggacagttt
      481 tctatgtagg cttcaaggta tccaattgag tcgttaagtt taccttgttt ttcagatacc
      541 agcgcaagat ttataagcaa atcaggcagc ttggtaatgt tataggcatt ttggaagtct
      601 gagcgggcgc gatcccattc ttttgactcc caatgtgtca gcgcactgtc gtaatgccca
      661 agcgctttgt cttgctcctg gggtgacaca acgggtggcg ctttgcactc gtcggctcga
      721 cgtactcggc tgcttttccg tcgggcttcg acatcccccg cgtcgcccac aaacaccaga
      781 ctggccatcc cgatgccaag cgcacatgcc caactcggaa ggcccaaaaa cctcgccatg
      841 cccatgcctc aaaatgacct attggtatta taaagccgtc agttgtctgc gacacatgac
      901 atttgtgcct cgagcgaaga gcatgagcac acaggtcgtg aatagatggc tgggtacata
      961 cgtcataaac ctatgtgccc gtcgctcaga tcgcgataga ttgcggtttt acttgtgccg
     1021 acgaaaaacg gcagataatc gaacaaggcg cagatgcgcc acgacagcca acaaggagcc
     1081 agcctatgaa agcacttcac tttgccgcga tggcctttgc cctgcttgcc gggtgcggac
     1141 catggtccac gtcgtctcat acttgcgtcg gtcacagctg cggatcgacc tggagcggcg
     1201 gtggcggtct tcccacgacg cagtattcga ccttcatcat cgacgccggg gctgccatcc
     1261 agttgccgca gacgacgctc ggcatcacca ccaacgggac gggcgactgg gtgctggcgt
     1321 ggcagggcga cacgtatgcg caccagtttc acggatcgat cacctgcccg acgagctgcc
     1381 agatcgccta tgcccggttt gacaacgcct atccggggga cagcgcgcag atcaccggca
     1441 ccaaccagat tgtctttcag ggcgtcacaa acgcagccat tccgcagaac ctgctgttcc
     1501 ggatgaccca gcagccggtg acgtttgacc tgtacatcga caaccagccg gcgattggcg
     1561 aagcggtgtt cgcgtcgcag ggcgcactgt cgacgaccga cacgatgccg ttttcgctgg
     1621 tgtcgagcaa cgtcgcctac gaaggcaaga ccgatcgggc tccgtcgttt gtcgccaagc
     1681 caccgcagga tggcgtcgag cgcgtgacta ccgttgctgc tccgccggca agctcgcagt
     1741 cagtggataa gtaggcccac cgctcttggc gatcggcgct ccgtggccac cacgaccggg
     1801 gagcgcattc cacagccgcg agatgatcag cgctcccagc acggcaaaga taagcggaac
     1861 atacggccag atttcaaact catggccgtg aagacccggt gccttgtagc gcaacagcag
     1921 cttgccaacc gcttttccgg ccagcgcacc gcctagatac tgggctccgt cgaacagtcc
     1981 cgccgccgtc gccaccgctt tgcgcccgcc aaagtccatt gatgccgcgc ctccgacgag
     2041 cgagtgcgca ctttggacaa aaaacgacac cgcaatcagc acgacagccg cgccccacgg
     2101 gttgtagatg gtgccgcgca ggctcagcat gcagaccgcc attccgagaa acgcgaaaaa
     2161 gatcaccgga gcgcgccgcg actgaaagag ccgatccgac gcattgccag cgaccaggcc
     2221 gcccaccacg gctgcaaatg gcatcgccac cgacgccatt tgatagggcg caaacgaaaa
     2281 cagctctcgt gggccgacgt cgaacatcga gctgatgtag cggggatacc agccgtcgac
     2341 aacgtggcgt accgaaccga tgcagaacga tgccagtgcg atgatccacg gcgctttgcg
     2401 cgtaaagact ttgcgcagca caaagccaag cgatgcatcg gtgcgagctt catcgggact
     2461 ttcgtcgccg gtatcgaact ccccgtggcc agcgagcgcc ggtgtgtttt ggatcaaaaa
     2521 gtagcaaaag ccccaaatca ccgtcagaag cagcgctggg acaaagaagg catactgcca
     2581 cggcacaatc agcagcagcg ccggcaaaag caccatcgcg agcgttcgcc caagctggat
     2641 catgatgccg aagatgccgg caaatttgcc gcgctccgtc acgcggaacc aggcggcgtt
     2701 tatcttcaca atcgacagcg ccccaaacga ctgaaagtag tgattgcagg cccacagcac
     2761 ggcgaacatc gcgatgaccg acgtgctatt gagaccggcg ccgagctgcg cttggcttat
     2821 cacttggccg cctgccatga ccgctggttt gacaatcagc aggtggcaca gaccaaacag
     2881 caggttgaag aaagcggcac cggcggcacc gatcaaaatc gctcgtttgc caccaattcg
     2941 gtccgcgagt ggtccgttta ggaacactgc acagccgtag gtggcaagtc cggcgctcgt
     3001 gatggtgcca tagtcatcat ggctccagcc gaactgctca gcgacggcgg attggaccgc
     3061 gccaaggttg tagcggctca tgtaaaagaa gccgtacata agcccgagga agaaccagtt
     3121 ctgaaagcgg cgcagccgaa actccggggt caggagttcg gcggaactgg cgctcggtgg
     3181 taagggatct ggcccgggac gcggggcagt aaccatggcg atgccatacc acagccgcgc
     3241 gcagaaacgg caggggtttt tttgccgagc acgctatagt gcgctcgcgc atgcccacgc
     3301 tgctgtcgac gacgtggtag ttagtccaaa tgcgcgagag aggcttccat gctgtcaaat
     3361 gcgaactcgg aacttccctg ggagcagcag gctctccagc tcgtcattga aaccgtgcag
     3421 gaggcgctgc atcaaaagtc accagcggag cagcagtcgc ttcagatggc gctgatgacg
     3481 ctgcgtggtt ttttggcggc gtcgcggctc acgcaggaag acggcggcac gacgtgggaa
     3541 ccgtatctgg aacccatttc accgccggac atgtcgcgga ttggggcgtg gagtcagacg
     3601 ctcaaagagc ggcgtgaagc ggcgtctttg agccgtcaca agctcgcgga gctgtcggga
     3661 attccggaag gggcacttga ggcgctggag agcggcacct atgccccgag ccagatgtcg
     3721 cttttgcatc ttctgtcggt gcccgagctg gatctggaaa cgcagtcgct gccgtgggaa
     3781 gcgagcgcgt ctgatccgaa cctggcgctc aactgctggc tcgcgccggg gtttgatccg
     3841 gtcaagatga tcaaagacct ggcgctgcaa gtgaacgggc acggtgggca tattgagcaa
     3901 agttacctct atatggatca catgagcgcc gctcactggt gcgccatcgc cgagcagaaa
     3961 gactacgacg cgatgcagtc gagcatgccg ctcgaaaaaa ccgcggcgcg gattttgcgc
     4021 accaatgcgc agtgtgggct cgacgtggtg gcgctgggac cagggagcgc gcgcaacgaa
     4081 gtgcggctgg tcaagcagct gatgacgcag tcggggcatg ccgatttgcg gctttatctt
     4141 ctcgacatca gccagccgct tttatcggtc gcctatcgtc acgccgcaga agtgctggcc
     4201 gacaacggcg gcgcggtgtt cgcgattcag ggcaactttc atcacctgcc gcgctacggc
     4261 cagcttctgt attccccgcg acgggcgcat cggcggcgga tcatcacgat gctcgggcac
     4321 acgttttcga acctggaaaa cgagctgcgg tttatgcgca acagcctgtg cggttttgcg
     4381 acgggcgatc tgctcgtgat ggactttcgg ctggcgtaca caagctcgtg cgatcccgac
     4441 gagatttatc gcaaagatcc gacgttcgcg agccgtcgca gcacggacct aagccagcgt
     4501 cgcgacaagt ttttggccgg tccgtttcgg cgctacggtg ccgatcaggt cgatgtcgag
     4561 atatcgcctg cgctcgacct aagctgcgca gtgcccggca gctatggcgt cgacctgcgt
     4621 gcgcgcgtgc gtggcgacgg tgccgatcgc gagttttcgg tctattacag ccgtcgctat
     4681 gaaccgaacc agctgttgca tgcgctgcgg cgtgagggct gggaaaccgt cgagagctgg
     4741 gaatatggcg gcgattccag cccgtgtctg ctcgtacttc tgcgcaagct cagctaacga
     4801 tcgcgacctg cccgtcaatc ggacggtgta tccgtcgaag aacccgaagc caaaaggaga
     4861 gacacatgcg gagtctgctg ctcatattca tggcgatgtt ggtgggttgt ggcgatgccg
     4921 aagtcggaca gcttccgacg gaagcaccaa tgctctcact gccaaacaag ctggggacgc
     4981 tttccgcgca gctgtcgccg cagacggctg ggccgctcga cggcaggcca taccagtaca
     5041 aagttcctag ccgctacgac aagacccgac cgacgccact tgtggtgatg ctgcacggct
     5101 tttccgcgag cggcagcgtc gaagaactgt atttgaacat ttcagccatc gctgaatcga
     5161 agacgtttct ttatgcattc cccgacggaa cacaaaatcc ggttggcctg cggttttgga
     5221 acgcgatgga gtggtgttgc aacttcttca agtcgggcgt cgacgacgtt gcctatgtaa
     5281 atgcggtcat ttccgatatg cagagccgct acaacgtcga tccgaagcgg atttttgtcg
     5341 tcggacactc gaatggtggc tttatggcgc atcgtgtcgc ttgcgagcta tcagacaaag
     5401 tcgcgggcat cgtcagcctc gccggacagc agtggaacga tccgacgcgc tgcaccccca
     5461 aacagcatgt gccgatcctg catgtgcagg gcacactcga cgctgtgatt ggttatttgg
     5521 ggatgccggg gctgtatccg ggggccaaag agacggtgca gatttgggcg cagaagaatg
     5581 gctgcggcgg acggctgacc tattcggggg aaaatcgcga tctggatacc gtgctgctgg
     5641 gcgccgaaac caccgtcgaa aagttcaccg gctgcccgag cgatggcccc gtcgaactgt
     5701 ggaccatcca cggcggcggc catgtgccgg tgttcaattc gttctttacg tcgtcgttct
     5761 acgacttcct catgtcccat ccgaagccgt agaccttggc gtccgatgcg agactacgcg
     5821 gggtcgggct tgggcgtggt cttgccgctc ggcggaagct ggggttcggc gatgtattcc
     5881 ttcgggatgt cgccggtcag gctgcccaaa aactcggcaa tcgactgcgc atccgcgtcg
     5941 gacagatcgc gaccaagctg gtgagcggcc attttcttga tggcgtccgc cagcttgtct
     6001 gccgatccgt cgtggaagta cggcgccgtc ttttcgacat tgcgcagcga cggaactttg
     6061 aacaggaact tgtcggcgtc gtttttggtc agcgccgcgc gacccagatc ggcctggttt
     6121 ggccacggct ttaccgcacc cagtttttga taggccgtcc cgccgagcag cgttcccgag
     6181 tgacacgcca cacagcccac gtcgaggaac ttgagcagac cggctttttc cacgtcattg
     6241 agcgcgttca cttcgccgcc gaggaacttg tcaaagcgcg agggcgtcag cagtcgtcgc
     6301 tcaaaagcac caatcgccgc cgtcgcgttt ttgatcgtca ccggctgttt gtcgccgggg
     6361 aatgctttct tgaatgcctc gacgtagccg gggatgctct gcagaaccgt ctcggcgcgt
     6421 ttgtgatcgg gcagcgccat ctcgacggga tttatcatcg gtccgccggc ttgttcttcg
     6481 acatctttgg cgcgaccatc ccagaactgg gcgacgtggc cagcggcgtt ataaaccgtc
     6541 ggtgcgttgc gtccgccgag ctgttttttg tggccgcttg aaaactttgc gccgtcgaca
     6601 ccgtacttgg ccaggtcgtg acacgagttg cagctgagat cctggttttt cgacagccgc
     6661 ttgtcgtaat agagctgccg tccgagcgcg gtcttttcgt ccgtcggagg attgtcggtg
     6721 ctgtcatacg acgctggcag cggtttgcca aacaccgcaa gtcgcgccgg gtcaatcgtc
     6781 ggggactttg gcggatctgc cggggctgct gctggcggag ccggggctgc cggggcttca
     6841 gcgggcttgg ccgcttcctg ctgcgggcag ccagaaagtg ccagcgtgcc aattgcaagt
     6901 ggcgccagaa aaagtgagcg agagacggag cgattgtgag cggacatgga atctcccagc
     6961 gcagttcgtt gttcgtcgtt tttggatgtc gtcgaagatc ggcttggctt catctgccac
     7021 agcgcgaaag atcgtccgag agcgatccga gactgccacc ccatttacca cagcactgcc
     7081 gcaacttgct aagatgaggg gtcgaagatg caggagctac ctgcgagcgg ggaggggcag
     7141 ctctcggtct gcccctattg cggcggcagt attgtcgagg gcgacgctca tgactgcgag
     7201 gagatgggca gcggtgacac gatgtccgtc gctgcgccgg tcaaaatcgg gaccgctcca
     7261 gtcacttctg gttctgccgg gggggcacaa actggaaaat cgacggctcc cttggcggtt
     7321 gcagtcggca aaggtgcggt gctcgtcacg ggcggtggaa ttgcgatagg cggcagtcca
     7381 ggcgcagcgt cgagtccggc caagtccgat ccgcaaaaac gtgcatcgag cgcagcgcgt
     7441 gaaaagttgt ctgcaagcga tgccaaaccg ccggccaaaa gtcccgtcgg ctcatcgacg
     7501 gacgaacttt atggactcat catcgacggg cgctacgaag tgctggaaaa agccagccaa
     7561 ggcgggatgg gcaccgtcta caaggctcgt cacgccacgc tgcgcaactt gctggcggtc
     7621 aagattctgc gcaaacccaa agacgaggtc agccgtcgac ggtttttgca agaagcgcag
     7681 cttctgtcgc agatcaagca cccgaacatc gtccaggtga tcgactttgg cgaacttccc
     7741 gacggcaagt cctatctggc catggaattt ctgtcgggcc cgacggtgag cgcggcagtt
     7801 cgcgagggcc agatgacacc gctgcgggcg tgtcgaatcg gcgcgcagat cgcccgcggc
     7861 atgcaccacg tccacgagca gagcatcgtc caccgcgacc tcaaaccgga caacattctt
     7921 ctcgtgaacc aggatggcaa cgtcgatttt gtcaagatca tcgacttcgg aattgccaaa
     7981 gacgtcaagg cggtgccgtc ggtccaggtg agcgggccga tgcgggtcag ctcggcgtcg
     8041 ctggcgctaa aatcgcagcc acagccgcca gcggaacccg agcctgagcc cgatgaagac
     8101 atgtcgctcg atgacacgcc ggggcaaaac ctgacccaag cgggccagct cgtcggcacg
     8161 ccgctgtaca tggcgcccga gcaaattcgc agcggtctgc atgatgccaa gggcgatcag
     8221 tattcgctcg gctgcatttt gtacttcatg ctgacgggac agccggtgtt tgccggcaag
     8281 cgggccgacg acatcatgtc ggcgcacgtc aagcagaagg tcgttccgcc acggcagcga
     8341 gtcccgggcc ttgtgataag cggcgagctg gaagcggtgg tgctgcgggc gctcgcgaaa
     8401 gatccggacg agcgatttcc gtcgatgcgg gccttggaat cggcgctcga gcagtgcatc
     8461 gagcagatcg cgccctcgct gccgagcgtc cggctgatcg ggcaaaaagc gaaacggttt
     8521 gcgccactgt gggtgccagt tgtcgtcggg ttgttggcgt tttttgtgta cctgtggcga
     8581 agccagcggg ggcgcagcga cgaagtgtcg caggaagagc ttctggcggt gcgggcacgg
     8641 gcactctcgg agctaaacaa acagagcggc tcgacggatc tgcgcttgcg actgccggcg
     8701 ctgcgggcgc tcggccaaag tcgcgatggt cagcagcggg cactgcttga aaagctgctt
     8761 gatgacagtg atccggcggt ggtggcgcaa gcggcggaat cgctggggct tctcggcgag
     8821 ccggcggcgg tgcaaacgct gctcatccac gttggtcggg cgagcgatcc ggcgacgaaa
     8881 accgcgctgg ccgtggcgct cgaacggctg ggcgaaccca aaggcacgca gcttctgtac
     8941 gaaggactgg cgggaaaaga caccgagctg cgacttctgt cggctctctt gctctgtgat
     9001 cgcggcaatc gaaccgcgat cgagcttttg ctgtcggtgc tggaaaaagg gcaggtcggt
     9061 ggtccgatcg agctgtcggt gctcggctgt ctggcgcgct cgggcaacga tgcgtcgtat
     9121 aaagcgctgc tggtgcggct gcagcgctcg gaaccgcgcg aggcccagct caaggcggcg
     9181 cagcagctta tcccgcttgg cgaagcgcgg gcgcaggtgc tgctcgacaa attggcggcg
     9241 gagcccggac gcgaccagtt gctggcggcg cgctatctgg cgtcgacgga gcatccggaa
     9301 acggcgcagg tatttcgggc tgtgataggc gatgcaaatg ccagcagcac agcgacgatc
     9361 ttggcggcgg aagggctggg cctttcggga cgactgcagg atgtacggct tctcgacaga
     9421 cagctgcgag gggcggggga cgaacaaatc aagctggcgg cagcggaagc gatcgtgcgg
     9481 ctcgcagcga cggatgttcg tcttatgtca cagcgcagct tgctgtgggc gcgcgacgca
     9541 ctgaccagcg gcagttgggc gattcgtcag gcgggtgtgg cgacgctgag cgaccgccaa
     9601 gaacaggaag tggggccgct gattgtgtcg atgctgcgtg atccggtgtc gcaggtgcga
     9661 aaagcggcgg tgcgggcaat ttctcgtcgc aacgatgctg gaatgctgga tgtcctaaaa
     9721 gcgcagctat ctgacggaga ttcgacggtg cgactggagt cgctgcaagc gattgtgcgg
     9781 ttggccaagc gactcctcga tggaggacag cgacccgcgg tcggtcaggt gagcgactgg
     9841 ctgcgtgaag tcgtgcaaaa aggcaatcag agtgaacagg tggtggcgcg cagtggtctc
     9901 cttttgcttg gggacagcag ccagaagccg gcgctgaagg aactggctgc gtccgccgaa
     9961 gaagaaaatc gcaagctgct ggccgagcag ccgggtgtcg acagcgaaat cctgacgggc
    10021 atgctgaccg atcgcagcga tgcggttcgt ttcgtcgctg cgcggcgtct ggccgagcgg
    10081 ggcaatgcga gcgccaagcc ggttttgcag gaaaccctga aaaaaggcac cacagagtcg
    10141 gtgacggcat cggtgctgct gaatcggttg ggggaaaaga cgcagcagcc gacgcaggtt
    10201 gcgacgtcag ttcccgtcga aaaccggatg cttctggtgg aatcagtcca aagtctggat
    10261 gccagcattg cactgccttt gctgctgcaa gcggcgcgcg atcctgaacc tttggtccgt
    10321 cggctcgtcg ttgaagtcgc ggcagatttg cccggcacga cgacggaaag taccccaggt
    10381 ctcccggtgc taaagctcct gtcgacggat agcgatgcgg cagtgcgagc gcgggtctgg
    10441 ggtcttttgt cgcgcttgca gtcggaccca catgcgacga aacctgcggc caaagatcgt
    10501 gtcgattcat cggcgagcaa aaagcccgat ccaagcagcg gcaatccgac ggtcaatccg
    10561 accagcaatc cgacggagca gcccgcgtcg ggacagggca agctggcgat cgtcgcggcg
    10621 ccgggcaccc tggttcaaat cgacgacaaa ccttggcaaa atgcgacgag cgctgggttt
    10681 gtccttccgg caggcaagca cacgctgcgt tcgctggcgg gggaacgaac catcgagatt
    10741 gtgtcgtccc agacgctgtc gctcacgctc cctgaatcgg aatccgagca gcagctcgcc
    10801 aagggcatcg acctgctcgg caagtcggat tatcgccgcg cccagaagct gttcgacaaa
    10861 gcgcatgggc agtgcaaaaa gaaaaaagcg ctggcgaccc cgtgtgagct gctggcgttt
    10921 gagggcggtt atcacttggc gacggcgctg gaatcgggtc atcgactgac cgaagcgatg
    10981 acccagtacc agaagctgct gaagagcacg ccgcggagca agggtcggtt cgatcggcgc
    11041 agcgaaatcc aggcggcggt gtcgcgtctg tcgtctcaga tgggtgaagt cgtcatcgca
    11101 agccgcaaaa agggcaagtg tcaacagtcg agcctgtggg tgcttcctgg gacgcacatc
    11161 atttccgtcg cagggaaaag ccagcaagtg caggtcagcg cgcagcagcg gctttccgtc
    11221 gggagctgcg agtgagtcat ccgctgcgct ggctgctggc cgtgctcttg ctttggcgct
    11281 gtgccgaagt gcaggcgctt gccagccccg atagtgaatc gagtcgccaa ggtcggcgtc
    11341 atcgacggag tgaacgaaaa cacgccacca aaacgacggc ggaccaccag gcaggcaccg
    11401 attcgacgga agaccgggcc ggcgatgttc gttcgcggcc caaagtgacg gcgctgctgg
    11461 acgaggcgcg aagtgcgctt ttggcacagg accccgacgg agcgctgaaa aaagcagagt
    11521 cagcgtacaa aaaacaagct tcgagcgaaa cgctgtgtct gcttgcgcag attgccgtcg
    11581 cacagaagcg ttttgtcgac gcacatgact tgtttcgtcg ctgtttggct gacaccgtcg
    11641 tgatggtcga cccggcagtt cgatcggaag cgcagcagat tccaggtgcg gcgctcaatg
    11701 ggcttggcga tctccatgtc agcggtgaca aagacgcgct ggtttacgtc gacgatcgac
    11761 tgcgtggcag ccttcctctg cttctgccgg tgcgggttgc gcccggtctt cacgcggtgt
    11821 cgctttcgtc gtctggccac tggacgcgcg gaaaagtcga agtaccggcg gggcaagcgc
    11881 gcgagatccg ctttgacgcg ctgagcggtg cggtgctggt gtcggttcct ccgatggtgg
    11941 tgtttgcgca ggaaagtggt acctcgcttg aaccccagct tttgtcggcg ctcgaaaaag
    12001 gcagtcagct cgtcggatat tcgctcacgc ttcgcgatgg aaaagccgaa gtctgtgacg
    12061 gacttttgcc cgagtgtttg ctggatgcgg cgcggcgcac gtcggcgggc tacgttcttg
    12121 cgatccgcgt gtcactgagc gcagctggca aagagatttt cctgtcgctg tgggatgcgc
    12181 aagtcggcga gctagctggg caagaaaccg cgcgctgtgc accgtgcagc gacgaagcgc
    12241 aactggcgac ggtggcggag cgactggcgg cagcgctcaa gcgggggcga tcgcggcctc
    12301 gcggggcact gaccgtgtcg agcattcctt cggcggcaca ggtcaagcta ggtggccgac
    12361 tgctcgggca gacgccgtgg aaaggatcgg tcttcgccgg gcaacagcag atcgaggtgg
    12421 cctttcctgg ctatgaaccg cagacgctga ttaccacggt ggcgcctgga cagcaggcgt
    12481 cgctgtcagc gctgctgtcg ccgctgcttt tggcggaacc ggggccgcgc ggctctgtgg
    12541 tggtgtcgcc tgcgcttcgt cgtccgatgt ggcgggtcgt gacgggggct gcggcgatgg
    12601 gcgtcggcat gttgatgatt ggcatcggtg gttccggtct ggccgcggat ggaacgtgcg
    12661 tgccgccgct tcagccacct gcgcaggtct gccgagagac gattcattcg gcgacagccg
    12721 gcggagcgct gcttggcgtg gggctttcgc tgacgattgg tggggcggtg ctcgtgggtc
    12781 tgccgccgcg gccagaagca gccccaagcg gtccatagaa gcccgctgcg cagcgacgcc
    12841 gaaaagccgc gctgttggga attgactttg gcccggcttg aaggctaaga ggcaaagcac
    12901 catgagcgaa gacctcgtcg tgctgcgcga aatcttgcaa cgcgagctgg agaccatcaa
    12961 tgcatacgaa aagatgcttg cgcggatctc cgacccagcc ctgcgcgaga ttatcgatca
    13021 cgtcaccgac gaagagcgcg agcatgttgc ggagatgtac cacctcatca tggatcgcga
    13081 tcccaagcag cgcgagcgct cagctcagag ccacgcgcag ctggtgagca tgtcgctgct
    13141 ccagactgcc gcggaaacgc cagccggtca tgaaccgaat gcggcgcaag cggcaggttt
    13201 atccgacaag ctgattgcgc ctgcgtcgcc cgcgcgacga gatccaatgc caccgccgcc
    13261 gccgatcttt cccgacgggg gctggtcggt ggggggtctg aagcgcagcg ccccgtaacc
    13321 ctgaagtcgc caagagagcg taccgctgtg cccgactcca tctcgatgag ccgcgcacgc
    13381 cctaactaag cgaagaagag acagccatgc ctgacgaaag caacttcctg acgcacgaac
    13441 acctgccctt gtctgaagcg cgctggcgcg aactgaacat gtcagtggtc gatgttgctc
    13501 gacggaagct cgtcgggcgg cgctttctgg atctgtatgg tccgctgggc gcgggcgtgc
    13561 agacggtaca caacgatgtc tatgccggca ccgagcgggc tgcgatcggc attctcggcg
    13621 aagaaccgat cgcgccggtg cgagccgcgg ggcgacagat cctgagcatt ccgattctgt
    13681 acaaagactt ttcgctgtac tggcgcgatt tgcagacgac ggagcagttt ggcatgccgc
    13741 tcgatctgtc ggcggcagcg ggcgcggcga gctttgttgc cgatcgcgaa gatgacatga
    13801 ttttcaatgg catcgccgac ctgaacttgc cgggtttgac gacggtgcca gggcgcacgg
    13861 tgatgccact tggcgactgg aaccaagtcg gcgcggcgct gcagtcggtc acggtcgcga
    13921 tcgagcggct ggtcgagacg ggtttttacg ggccgtactg ccttgtcacc agcccatttc
    13981 tttatgcaaa gctcgcgcgc tttgtcgaaa acacaggcgt gtcagagctg ggtcacatcc
    14041 gcgagctgtg cactgacgga gtgttccgct cgagcgtcct gcctcgggac cgcgctgtgc
    14101 ttctgtcgac tggcccgcag aacttcgact tggccgtcgc acaggacttc agggtggcgt
    14161 atcttggggc ggagcaaatg aaccatccgt tccgcgtctt cgagtgtgcg gtgctgcgca
    14221 tcaagcgcgc ccaagcgatt tgcacgctcg aggccaaagc ataaccaaac ctagatcgaa
    14281 cacacgagga ggggaccaat gttggtttcg gagcaagagg agtggccgtc atggcttgtc
    14341 ccaatgtcag cgatggatga ggacgaagac gaggactttg aagacgaaga cttcgacgat
    14401 gaagacttcg acgacgaaga ctcagacgaa gacttcgatg aagacgatgc tgacgacgaa
    14461 gaattcgatg aggatgacga cgaagacttc gacgaagacg acgacgaaga agccgaagac
    14521 gacgatgaag acgacgatga ctacttcgac gaagacgacg aggatgaaga agacttcgac
    14581 gaagacgacg aatagcggcc tgcgtctttt ggcgcggggg gcttagcttc gtcccgtttt
    14641 gggtgccaca cagggagtca cgatgaaggc tgaagactgg aacgtcaccg tcgggagcgg
    14701 caaagatcag cgcaagctcc aggtcaaaat cgaaagccac gaaggagacg tctatcgggt
    14761 ccggattggc gacgagacaa cgactgtgcg ggcggtgcgg atcgaaaacg gcagttactc
    14821 gctcattgat gaaatggggc gacagcgcgg gatcgatttc gacgggatgc tgccagaagc
    14881 caagctgagc atcgacggtg gtgatgcgat ttcggtcgtc gtccaagatg cgcagagtct
    14941 cctcgacgcc gcggcagccg atgcctccgt cggagagatt cgcgcctcga tgcccggcaa
    15001 agtcgtcaaa gtgctctgca aagtcggcga cgtcgtcaag tccggccaag cgctgctcat
    15061 catcgaagcg atgaaaatgg aaaacgagct gcgcgcgacg ggcagtggca aagtcgagtc
    15121 gatttccgtg cgcgaaggac agaccgtcga aggcggccag tcgctgctcg tgctgggagg
    15181 cgtgtcatga gcattcgcag gtggttttcg ttgttgcttg tcgtcgtggg tgtggccggc
    15241 gctggctgtg gcaaagacga cggctttgtc cgcgacgatg gacaggcggc gtttgaaggt
    15301 cggaacatca agttccagag cacatacgtc tattttccgt cggatttttc gctgtcgatc
    15361 gtgcgcgaca cgatgccgca agacgaagtc tgcgcgatcc tcaaacgcta ttcgagcacc
    15421 acgccgaagc tgcccggtct gcccgttctg ccgtacaaag acgaacacta cgcgctggtc
    15481 gtcacggtcg attcggtgca gcctggcgac gatgtgtcgt tcgatgtgac cgatacgggc
    15541 ggtgctggcg acatgcgcta tgcggcgctg gcccactacg gtgttggcaa aaaccgcgac
    15601 tccgattggg cgctgcgttc gggcggttac gttcgcatca ccgagctatc ccactacaaa
    15661 catgtcgcgg gcaagctggc gcttcgtttt ccaagcggtg aaaagctcga tgaggacttt
    15721 tccatcgacg tttgccctcc gtaaactcgc tttctgctgc cccgctgcca tcgcaacgca
    15781 acaaagtttc gggcccagcg cttgacaggc caagcacccg tacttagagt gcgctgcggt
    15841 tttctgtcct gcaccatgca tcgcaaatcg tctagtagat tgggaattgt gggatgacga
    15901 cacacgcgca aggcaagacc atgatccacg tcgacggcct gaccaagcac tatggcaagg
    15961 tcaaggccgt caacaacatc tcgttcgatg tgcacgccgg tgaggtcttg ggtttcttgg
    16021 gcccgaacgg ggctggcaag acaaccacga tgaagatcct cacctcgtac ctgtcgccga
    16081 catccggaag ggctgtcgtc gctgggtgcg atgtctttga tcaggcactc gaagcacgcg
    16141 cccgcatcgg ttatttgccg gaaggcacgc cgctttaccg ggacatgacc gtgctggagt
    16201 acctgaactt catcgcagac gtgcgcaaca ttccgtcggg cagcaagcgg acgcggcggc
    16261 tgcgggatgt cgtcgagcag tgcggcctgg gcgaccggct cggcttcctg atcggcgagc
    16321 tgtcgaaagg actcaagcag cgcgtcgggc tcgcacaggc catcttgcat gagccggaag
    16381 tgctgattct cgacgaaccg acgagcggac ttgatccgaa ccagatcgtt gaaatccgtc
    16441 aggttatcaa gcagatcggc gaaaagaaga cggtcatctt ttcgacgcac atcatgcagg
    16501 aagttcaggc gacctgttcg cggatgctga tcatcagcaa aggcaagatc gctgccgacg
    16561 gcaagcccga tgaactacag cgcaaggcgc aaggccaaat gtcgctgcaa gtcgagctgc
    16621 ttggcgacga aaaccaggcc aagagcggcc tcgagtcgat cactggagtc gagtcggtgc
    16681 gtgcgttgcc gggcgaagag agcgagcttc cgggtggaca tcgctttgtc gtgatggcca
    16741 agtcgggcag cgatccgcgc gccgaaattt ttaagatggc gacggccaaa ggtctccagc
    16801 tcaccgagct gcatcgcgag cgggcgtcgc tcgaagatgt gttccgcaac ctaaccgcgg
    16861 gctctgcacg ctagtccctg ccgaggagaa gcagacgatg cgaaacatga cgaccatcat
    16921 gcgccgcgag ctgggcgcgt acttcaattc accgatcgcc tacgtggtga tttcggtgtt
    16981 cctggttctg tcggcgtttt tgttctttaa cgccttgttc cttcagcagc aggccgatct
    17041 gcgcgagctg ttcgatggtt tttggaccac gctgctcgtc gcgattttgg gtccggcgat
    17101 cacgatgcgg ctgtttgccg aggagcggtc gaacgacaca ctcgagctgc ttttgacgat
    17161 gccagttacc gactgggatg tggtgcttgg caaatacctg gcggcacttg tgctgtttgg
    17221 ctacgcgatg ctggcgatgc tgtgtttcgc ggtgtcggtg tcgcttctgg gaccgctcga
    17281 caaaggcgcg gcgtttgccg gttttgccgg gctgtacctg acgacggcga cgtacatggc
    17341 gatcgggctt atggcgtcgg cgtttaccaa aaaccagatc gtcgcgttcc tgttggcggt
    17401 gacgctgtgt cttctcttgt ggctcgtcgg caaagtggtc caagtgatgc caccggcgat
    17461 tcagccgttt gtcgattgga ttggcgtcga cggtcacatc ggcaacatgg cgcgtggtgt
    17521 gttcgacacc cgcgaccttc tgtacttcct gaccatcatc gtctcgtgtc tgtttgtgac
    17581 acagacgacg cttgagagcc ggaggtggag ctagtcatga gcaagccact tcacaaaagc
    17641 cgcacggcgt ttaatgccgc gctgtttgtg ttcctgtcga ttggtgccct gggctttgcg
    17701 aacgtcattt cgctcaagca ctttggccgc atcgatctga cgaaagacaa gatttacacg
    17761 ctcgccggtg cgtcgcgtga tcttgtcgcc aagctgccgg atcggatgaa cgtcaagctg
    17821 ttcatgagcg aggatctcaa gccgccgttc aaacagcacg cacagtttgc gcgcgatctt
    17881 ctcgccgagt atgcgacgta ctcgcagggc aagttcaacc tcgaggtcat caagatcggc
    17941 gagggtgacg aggcgcgcaa aaaagaagaa gaagccacca agtgcggtgt gcagaaatcg
    18001 aaccgcggcg tggtatcagc caacaagttc gagattggcg cgacgttcct cggcgtctgc
    18061 tttgcatacg gtgacaagac agaagcgatt ccggtcatcg accgcgacga aggcctggaa
    18121 ttcatgattt cgggtctcat caagcagatg acggtgaaga aaaagaagat cggctttacg
    18181 acggggcagg gcgagcttgg ctcggggcac ggcggcggtc ttcagcttct gtcagagcag
    18241 ctcaaggcat cgggatacga gacgatcaac gtcgagctga aagcgatggt gtcgtcggac
    18301 atcgaccttc tgctggtcat cggtgccaaa cagccgtttt ccgagcgggc caaatacgtg
    18361 ctcgaccagt tcctgatgtc gggcaagtcg gtggcgttcc ttatcgacgg acagcagatg
    18421 cagacgccgc gcggtcagat ggcgccgggc atggaaatgc cgcagattgc gcaaaacaac
    18481 gacgtcggac tcgacgatct gctcggtgcg tatggcgtca agatcaagca ggacatggtg
    18541 ctcgacaagc gcaacttccc aggcctggtg aacgtcggtc agcagatgta cctgatgaat
    18601 cacccggtgt tcctgaaagt gacgccgctg ccgcaggtcc acgcggtcaa cgaaaacatg
    18661 ccgggcgcgc tcttgtcgta tgccagctcg gtcgagctcg tcggtgatgc caaagacggc
    18721 aagggtccgg tgcagtttgt gaagatcctg tcgtcgagca accaaagctg gcgtccgtcg
    18781 ggtccgtttg tgttcatgcc gggtccgaac cagacgccac cgacgccgag cgaggatcgg
    18841 gggccgttta cactcggtgt gattggtcaa ggcaagttca agagcgcgtt tgccggcaag
    18901 ccgatcgtca aggaagacgg caccaagatt cccgccgatc agagccagcc gggcgtcgag
    18961 ccgatgaaga gcgaagcgca aaatggcgcg cggcttgtcg tcattggcga ttcggacttc
    19021 atcggcgacc agttcatcgg aaccggggcg cgggcgggtc tgcgcgacta cctggcgaac
    19081 cttgtctaca cgatgaacat catcgactgg ctcgcacagg acgaagcgct ggctgcggtg
    19141 cgcaaaaaag gcatgcagag ccgtccgctc gatcaagtgt ctgagagcac cgtgacgctg
    19201 gtcaaactgg tcaacatcgg ggcgctgccg ggtctgatcc tgctgatcgg cctgattcgg
    19261 ttccgcatcc ggcagagcgc ccggcagagc gccaagctgt aaccacaccg aggctcacga
    19321 tggaaaaaaa gaacctctac gcattggttg ctttcgtcgc gcttgccgtt tctgcggtct
    19381 attcgctgcg ttcgcccgac aagggagaac gggtcggcga gcgaccgcgc gcgattgccg
    19441 agatcaaggc aggcagcatc gccaccgtcg aagtggcgca gcccggagac aaagacaaag
    19501 tcacgctgac caagaagggc gacaagtggc aggtgacggc gccttacgac aagccggcgg
    19561 atcagacggc ggcgaagtcg gcggcggaag cgctcgaaaa agtccgctgg g
//
LOCUS       DAORGN010000080        19476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1104_length_19476_cov_6.033160, whole genome shotgun sequence.
ACCESSION   DAORGN010000080 DAORGN010000000
VERSION     DAORGN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1104_length_19476_cov_6.033160"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKL17_10640"
     CDS             complement(<1..1113)
                     /locus_tag="PKL17_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45232.1"
                     /translation="MHAIRSALPNTFAFTVGKSLCTVPALLALFSVGCDGNMMTNTPP
                     PAPTYLAVTASDFVAGTGTLSLIDPATLQVRKAVDQVDPSAVVRAFDKKLYIIDSTHG
                     SLRIYDAVDNFATPKDYPLRKTGVVDATQGNPHDIFVDNATGKAYVSLYGSFGSTQVK
                     ATTALAVLDLNNLAAGISSFVTLTVNPADTDGNPDASQLVGCGGSLYVLLQDLDRNAG
                     YKPVGSGRLAKLNLASPQTASYIALAGENPTAMAVYGNCTEAIVGSAGDQLGGTLTGK
                     SGIERVDLSAAKTLGLQLTDSALGGNVSTLDASDGQHVFADLSVKSGTAYNNTVYAVD
                     AVAKQKGAAVLGPMSYVPAVAVLGGQLVVLSGGKAGS"
     gene            complement(1146..3242)
                     /locus_tag="PKL17_10645"
     CDS             complement(1146..3242)
                     /locus_tag="PKL17_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45233.1"
                     /translation="MHWSTHTKRPFCLLLLCLFCEILTFRMGAGRAQTTPFQTTVSGD
                     SVLQKSDSAAAVSVIELGLPGQASAPSAAKSSVGELIVGQPGVQVRRSGGLGQWSGAS
                     LRGAAPGQVAVFVDGVPLSRGSQSAVDLSQLPLDGLERVEVYRGVAPLEFGSETLGGA
                     INLVTRRGQGPPSAWLSLGSGSFGLRRASVGYKAPSPRLLLSAAYQAADGDFPYFFNE
                     GLRYGREQLDELRRKNDDFSQISVDARIEAETRAGSLYVHSSGLLRRQGVAGIGQPSS
                     QPGQPDLQTGRVLLDGGVRLRTPTPRVQIDLDVHGLVDRTQSKDLLALPTVLSEQLSQ
                     QAGAKAFLRIYTGPAEDLKKSQLQTVAEVRYEQNRQRDLCPAPRLDCDRSQAAQSDRL
                     RGAFSIGGELLLADRRLRFEPGLHLLIVRSQYQSLGDRETESMPTDVSVLPSPRAAAR
                     VLLRPWLLLRASSGRFVRLPTFLELFGDGAFFRKSLGLRPESAWSGELGVEASGQLAS
                     FLTANLSLHAFGRNIDDLIDVVRDGPTLRARNVGQATSAGVELSGAANLHELLRVELS
                     YTFLDSRDRTTQPGRSGNLLPGRPQHAVFLRSEAAYRPLRLFYELDFVSSLFLDPANL
                     VERPARTLHAVGVALGPITRFRVSLRVEVRNLLDTRTVDMALPLEPKPRPVPLTDFFD
                     YPLPGRALYATLAGRL"
     regulatory      complement(3260..3442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3538..4092)
                     /locus_tag="PKL17_10650"
     CDS             complement(3538..4092)
                     /locus_tag="PKL17_10650"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNK45234.1"
                     /translation="MSRTPCRELAAAPPSEAETLFCFPPLVSEKTKLLCLGSFPGAAS
                     LAAQQYYAHPQNHFWPIMQAIWPTIPMAKDYASRCDWLQERGVGLWDVYASCSREGSL
                     DRAIRNAVVNDFASLQARCPKLQLIAHNGGESFRYAKVVRASLGQSHFPLIKLPSTSP
                     ANASWSFARKLAAWQKAVQSAGLL"
     gene            complement(4118..4975)
                     /locus_tag="PKL17_10655"
     CDS             complement(4118..4975)
                     /locus_tag="PKL17_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45235.1"
                     /translation="MRLTSLVTTLSLTATLCPLPALAQLPPPPSVSTQPSPQPYPQPA
                     YPQQTPNAYPQQYAPQQYAPQQYAPQQNPSAYPQQYAPQPYPQPQYPSYPQQPIVELR
                     SNDPAATLFMVTGGGTRMVWGRHHRYLSHYDVWSPVCRTPCGVPIDPSGLYQIRGLRM
                     NASSSFVIPQTQLVTLDVDAGHSGPRAGGIVLTTFGGIVTVLGITFAGIGIGQANANY
                     DARVGQSSSSGFLVAGGTMLGIGTAMLAGGITMIVLSRTTVRISGGSRLAQTQSLPRI
                     ALAPDGIHF"
     gene            complement(5069..6544)
                     /locus_tag="PKL17_10660"
     CDS             complement(5069..6544)
                     /locus_tag="PKL17_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNK45236.1"
                     /translation="MMSPDEVRVQLIHFSDYHSHAIPFYSEHKPDQGGLARTLAYIAG
                     ARAKDQSVIALSGGDMWNAGTPAWSDKYYDGCADWKWLGEYVSVMAFGNHDVDYGWDA
                     LKNCQKQSGIQILSGNLVDGNGQRLLDNQGLPYVVKNVGGVRIGIFALSGSDFVRLVK
                     STNLPTGAKFLDPIPVAKEIVTQLREKEQVAAVVFIGHQDRDSDFAMAQAVPGIDVIL
                     GTHSHYKGTFAQIPGTQTYFISPYQYLSYLSHVELLFRSGKLAGVTGKLVRMDSQLPQ
                     DATVSSTTHRMQIDLEGDPKYAKLFEKIGSAATELDLDGIDQNESPLGNFVMDIVRDA
                     AQAQFALSSASSFRASIPPGPIRNEDYLTALPYKNKILTFQMSGAQLQAVLDLAATRR
                     GSDLFGVTGGVRFTIDGNRATQVTVRKSATEADQQPIRMDATYTVMATDYMANVATGY
                     KDLFAQVGPAKDTGKIVNDVVIDYIQRKSPVTAQRDGRIKP"
     gene            6572..6706
                     /locus_tag="PKL17_10665"
     CDS             6572..6706
                     /locus_tag="PKL17_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45237.1"
                     /translation="MTSGHSTERESQGGERAAAQKHMGKDSRGLAVSHDRASYIVSRK
                     "
     gene            6785..8800
                     /locus_tag="PKL17_10670"
     CDS             6785..8800
                     /locus_tag="PKL17_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45238.1"
                     /translation="MQTTAYAQASDDAGGKAAAEDRFSSRVVADRVKVDEPEAIVGEP
                     VRTQPGTLGDPFRVVSLLPGVVSPFPGLPLWSIRGASPGTAGFYLDGLRLPQLFHLLI
                     GGGVVHSDLIDRVDFYPSGFDVTMGKAAGGIISASTRPARSDGNHFDLSLRLYDVGGL
                     VELSLPKEIRISVSGHYGYPGPILRAIDNRIQLDYWDYQVRLDWRFLTFQVLGGYDAL
                     ALSLGKELEYSSPQSTRTMFHRAQIRLHGESGKVSGEVALFGGYDEAGDISDRGVNKL
                     MAGARGFVRVRLPRLSFQIGTELEVARFSGERFDVGLRSLAFVRNAEGPARVSGKDPD
                     ATPDELGEIGNNRVGITSSFYLHSELSLWPQRLYLTAGGRVDIYHVGDETLIGFDPRA
                     QLRFVATPWLSLQASIGVYQQPPSFPVLLPGVDTFALKLGLQRAQSTVIAEEMSLPWN
                     LSLRLTGFHQRFSNMTDLPPLGSSVCASPPPEKLHGTTATLVRVADGQAYGMEVWLRR
                     SVGLLTGWISYTLSRSERSYPCGVRPADYDQTHVLQVVAQTRLPRGFVAGVRLQFSTG
                     RPETLVDVGADNPSLEQIAERTGGQGVRNNFRLPNYVQLDVRIDKRWTFRRFYLHAFL
                     EVVNATFSRTNLLLAYPSPDEQANGKHDPELIGFSWILPSIGLRGGF"
     gene            8890..9849
                     /locus_tag="PKL17_10675"
     CDS             8890..9849
                     /locus_tag="PKL17_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45239.1"
                     /translation="MYHLLARLRVLHEALTPEVDRFLPPDLSPNYTPCVSSQHVFCGP
                     EGRLISIGMVRSDDSGTIALRLVRERTDGGLDGLSLVAPHALGASFQQDGLLPGRMST
                     NRLLLSRGEHAIAGMYQSEDQTKPIHSVRFSLTLKPLERGVGIGQLGLRLGHLIATDF
                     LRVLYRGELFLNGEKLQIDSVGSLSLHAGDRLPQYGYLVTSPHAASGLLPALLLLAVH
                     DDSLRVLGESVGGQSVIYGLGRNGLPKVALHLGSLGPALPLGDDGKLLFHDLRPFGYD
                     FLGQPTVTALGDARFVSQQGTVRDLGRVFVDCRGAHFVTLLHS"
     gene            9911..10276
                     /locus_tag="PKL17_10680"
     CDS             9911..10276
                     /locus_tag="PKL17_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNK45240.1"
                     /translation="MIDHISLAVSNLDASVRFYEKALAPLGYAVLLSFPGVAGMGIAP
                     KPDFWLGQKESADSGRHIAFCATSRAQVDAFYQAAMAAGAKDNGGPGLRPQYHPNYYG
                     AFVLDPDGNNIEACCHGPA"
     gene            complement(10283..11284)
                     /locus_tag="PKL17_10685"
     CDS             complement(10283..11284)
                     /locus_tag="PKL17_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNK45241.1"
                     /translation="MSAILSVRPLGFTWDTVDPFLFCVHHNDDYPQANEQMGPTASLA
                     GRDIGQDFAGKDGFRMYHGDVVPGFPNHPHRGFETVTIVRRGFVDHSDSLGATARYGR
                     GDVQWLTAGKGIQHAEMFPLLDRNARNPIDLFQIWLNLPRADKMVDPHFSMLWANHIP
                     HVLTPGVDVLVVAGALASQKPLPPPPHSYASRRESEVAIWTVKLAPGAKWSIPAASAG
                     VRRTVYFYSGPSLSLCGHPITARHLVEIRADVEAPIQNGEAESELLILQGRPLGEPVV
                     QHGPFVMNSQADIRQAFADYQRTQFGGWPWKRRDPVHPRDAGRFAKHVDGRIEKADS"
     gene            complement(11306..12184)
                     /locus_tag="PKL17_10690"
     CDS             complement(11306..12184)
                     /locus_tag="PKL17_10690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNK45242.1"
                     /translation="MRFRTKPRQGLLGIGLVAALSGCLQLGGFSSGQIDLSALQSAGQ
                     KIEAANQPVPEDQEVEMGRHMAALLLGASPLEQNPAAQRYVNQVGRWLTLSTARPQLD
                     WHFGILADADINAFAAPGGYVFVTRGLLDLLTNEAELAGVLAHEITHVTAQHHLEAMR
                     KENMMGAAADVGSMVGNNTPLSRFGSGTREFAQKVVSSAKQLYVRGLDKRDEFASDEE
                     ALRLMSRAGYDPYAFLAVMQKLESILGSSSRLALLLQTHPSPTARLEAMDKTLRTLAM
                     PQWPALGEARFEQNIR"
     gene            complement(12189..12722)
                     /locus_tag="PKL17_10695"
     CDS             complement(12189..12722)
                     /locus_tag="PKL17_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45243.1"
                     /translation="MTHFPKAAFPALCLASLTAAWPAMLCADSGQLVTKTELRAKAEA
                     SAPAVAQLAAGTAVDIGGRQGAFYQVKTAAGEGFVRMLTVRLTSVVRTSEPPPSGVIA
                     LSPAARSSTTVATGIRGLSRTDIERANENPAAVAELERYRVTLEEARQFGAAANAQAV
                     QSPASEAAQAVPQKGMR"
     gene            complement(12719..14578)
                     /locus_tag="PKL17_10700"
     CDS             complement(12719..14578)
                     /locus_tag="PKL17_10700"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK45244.1"
                     /translation="MFVSVHPTLPMRPPFASAISLRLALVLAQLLLVLPLFFSSARHV
                     DFLWLDRLTRQAAARAHADPDIVIVDIDDYSLQAMRDSVGRWPWPRATHAELIEWLTA
                     QNVRTIALDIWFSEPDLQRPEFDTYFGEVIDRSSHLFLPLLALKSDRALRLLSSYPPS
                     LPITRSRDADPQAKLDLLLPAMGNPAHWRLGLINYLADSDGIGRHYGLTAEPHPGWRI
                     ASLPLVLAQAQGFAVEGIPDKLRLSFIGQKGESPYRKLSYADLWLQVQKGEQRKDLAG
                     KTVFVGSTASGLHDLRPTPIGAQYPALYLLANAYDNVKNRTWLRHESWYGPAVGLPFL
                     LALYAALAKKKSLTKTALFGVLLGGLLVFASVFAMRLRILIPVLTPILSMMVLLVVAT
                     ADRYWQERKARQATVDLFGRFLDPVVVAELAEQGLRPETLDGRQCDITVLFSDIRGFT
                     SMSETAEAAEIMKLLNAYFTRQVGVIFRHHGTLDKFIGDAIMAFWGAPVADPQHALHA
                     VEAALDMVDELDAFRKERNLPGFDVGIGIHSGPAVVGMLGSQQRMEYTAIGDTVNLAS
                     RLEGTTKGIARILVSHKTRELCGDALLFVAHGAHKVKGREEPVLVYEPRRKQA"
     gene            14664..14816
                     /locus_tag="PKL17_10705"
     CDS             14664..14816
                     /locus_tag="PKL17_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45245.1"
                     /translation="MTEPAGGTGIASASATWRAADFAGHKRKQSEQRAGKTSSGQSTL
                     QTGQRL"
     gene            complement(14845..15714)
                     /gene="gluQRS"
                     /locus_tag="PKL17_10710"
     CDS             complement(14845..15714)
                     /gene="gluQRS"
                     /locus_tag="PKL17_10710"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNK45246.1"
                     /translation="MVTRFAPSPTGYLHLGHAYSARMGFDAARRHGGTFLLRIEDIDQ
                     TRCRPEFVDGILEDMHFLGLRFPTPVRKQSDHMADYEAAIQKLLALDVLYPCFCTRAE
                     IAAELQAAQSAPQDGAQAAPIYPGRCRSLVPEEAAARMSQGHNYALRLSTEKTERRLR
                     DLGRWPLDFFDTHHGTVAVSPSLVGDVVLARKEVRTSYHLAVVVDDAIQQVTLVTRGE
                     DLFLSTHVHRQLQALLDLPTPRYAHHPLLRDVAGERLAKRRGSESIRSLRQKGMSADE
                     MWAKIGCEPLPAV"
     gene            15794..>16479
                     /locus_tag="PKL17_10715"
     CDS             15794..>16479
                     /locus_tag="PKL17_10715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNK45247.1"
                     /translation="MTNPPAPVRIRFRPWLLAAVAVLWTVILLGFLFRFAELPSLTAY
                     LGRLWRGPLVQLQIRAVWMGFHLVGLGLCSFLFARRLGVSATDLGLSISALRNERNRI
                     VWITCGCLPIVLALSFVPAFQRAYPMFRSSESGVFVTLLWLGIYALYLFSIELYFRGF
                     LLALLSPLWGGLAVGIAMIPYVLTHRFGPEIVGSVPVGILLGIWRQRCGSIIPGLLTH
                     LLIATEIELCG"
     assembly_gap    16480..16579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16796..17794
                     /locus_tag="PKL17_10720"
     CDS             16796..17794
                     /locus_tag="PKL17_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNK45248.1"
                     /translation="MKTHRLDLAFLRILLVAVPFGCGPKKFTEPAPTGSCEANQIVQL
                     SDIAARDGGSGGDGGVRFSGDGGVSVANTLSDADCIQICTSTSTCQPIVNDGGTVTSV
                     ECIYPCYAFGCGRRPQSLPLVRSGHPSALVRYLEEAAYLEAASVFAFRRLARELLAHD
                     APSWLVAWADRAANEEVRHARVMARLCRRYGGTLTMPVEGPQGVRPLAEIARENAQEG
                     CVRETYGALLLWIQSASATQPHLQRVLQVIAREETEHAKLAFSVADFCASRLSDAERA
                     EVDRAQQAAVADLGEAIAQNPPVELQQRLGLPPVELAQQLYQSASRELWTMTATAC"
     gene            complement(17781..18317)
                     /locus_tag="PKL17_10725"
     CDS             complement(17781..18317)
                     /locus_tag="PKL17_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45249.1"
                     /translation="MQRLLLCACLLFGCGNYPTAQSFVPSNSSPLRLPPEVPQGTPAF
                     LISANASVDVPYGAFGITTDGNGWYLEWQTESSARFSGEVFCPQTCVQSTIYFKSSPR
                     GSVTVLTPYHFTFDAKTEANTPVHLEFRATEQPVVFSLQIDGEKATNPKTVFASDQKV
                     SSVSAMPFALVSSNVSKL"
     gene            complement(18364..18906)
                     /locus_tag="PKL17_10730"
     CDS             complement(18364..18906)
                     /locus_tag="PKL17_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45250.1"
                     /translation="MNIASLLDKAQQMMAVAFQLKTEAQSYLQKLPGFSFDETMAGPI
                     TLDGSQHPRHMEFRIHAEVPSLGEYLEEGRTDIAGTVSISGFADSAALSGSLWILPLQ
                     RQIRYEFGFAASDGSPYRFAGQKDISPLDPVTTLTVLPAYLYDAHGRETGQAEVRFAL
                     GDLPSFLASWRPVLAGNSRR"
     gene            complement(18953..>19476)
                     /locus_tag="PKL17_10735"
     CDS             complement(18953..>19476)
                     /locus_tag="PKL17_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNK45251.1"
                     /translation="HRLDADTSGALLFAKTPAAWTKLRADFAGGLVEKEYLALVAGSP
                     DDDEFDVTLPLLAGRGGSPKMHVATTPEEIYHPDALDAHTAFFVERRGPMHTLLRVQA
                     RTGRRHQIRVHLAHLGLPIVGDPLYGQGEPGGQFLHASLLRFVSPSDPSRRISVTAPL
                     PPDRAATLQRLLP"
BASE COUNT         3768 a         5665 c         5888 g         4055 t
ORIGIN      
        1 gctgccggcc ttgccgcccg atagtacgac cagttggcca ccgaggacgg ccactgccgg
       61 aacgtagctc atcggtccca gcaccgccgc gcctttctgt ttggccacgg catcgacggc
      121 atagacggtg ttgttatacg ccgtgccgct cttgaccgaa aggtcggcaa agacgtgctg
      181 tccgtcgctg gcatccagtg tgctgacgtt tccacccagc gcgctgtcgg tgagctgaag
      241 cccaagcgtc tttgccgccg acagatcgac gcgctcgatg ccgctttttc ccgtgagtgt
      301 gccgccgagc tgatcgcccg ccgaaccgac gatcgcttcg gtgcagttgc catagaccgc
      361 catcgccgtc gggttttccc cagcgagcgc aatgtaactg gccgtctgtg gacttgcgag
      421 gtttagcttg gctagacgtc cgctaccgac gggcttgtag ccggcgttgc gatcgagatc
      481 ctgaagcagc acatacaagc tgccgccaca accgacgagc tggctggcat cgggattgcc
      541 gtcggtgtcc gccggattga ccgtcagcgt cacaaacgaa ctgatcccag cagcgaggtt
      601 gttcagatcc agcaccgcga gcgccgtcgt ggctttgacc tgcgtcgaac caaacgaacc
      661 gtacagcgac acataggctt ttcctgtggc gttatcgacg aaaatgtcgt gtggattgcc
      721 ctgggttgcg tcgaccaccc cagttttgcg cagcggatag tctttcggcg tcgcgaagtt
      781 gtcgacggcg tcataaatcc gcaagctgcc gtgggtgctg tcgatgatgt agagcttttt
      841 gtcaaatgcg cgcacgacgg cgctgggatc gacttggtcg acagcttttc gcacttgcag
      901 cgtcgcagga tcgatcagcg acagcgtgcc ggtgccagcg acaaaatcgc tcgcggtgac
      961 agctaagtac gtcggtgccg gcggcggtgt gttggtcatc atgttgccat cgcaaccgac
     1021 ggaaaacagc gccagcagag ccgggactgt gcacagggat tttccgacgg taaacgcaaa
     1081 agtgtttggc aatgcagaac ggatcgcgtg catggatgct ccttgggcgg acgtgcgccc
     1141 agtggttaca gccgccccgc cagcgtcgcg tagagcgcgc ggccaggcag cggatagtcg
     1201 aaaaagtccg tcagtggcac cgggcgcggc ttgggctcaa gcggcagggc catatcgacg
     1261 gtacgggtat cgagcagatt gcggacttca acccgcagtg aaacccgaaa gcgggtgatc
     1321 gggccgagcg ctaccccgac ggcatgcagg gttcgcgcgg gcctttcgac gagattggcc
     1381 gggtccaaaa acagcgaact gacgaagtcc agttcgtaaa agagccgcag aggacggtac
     1441 gctgcctcac tgcgcaaaaa caccgcgtgc tggggtcgtc cgggcagcaa gttgccgctt
     1501 cggcccggct gtgtcgtccg atcgcggctg tcgaggaagg tgtagcttag ctcgacgcgc
     1561 agaagctcgt gcaggttggc ggcacccgaa agctcgacgc cagccgacgt ggcctgccca
     1621 acattgcgcg cccgcagcgt cggtccgtct cgtaccacat caatcaagtc gtcgatgttg
     1681 cgcccaaaag catgcaggct caggttggcg gtcaaaaacg acgccagttg cccactcgct
     1741 tcgacgccca gctcgccaga ccacgccgat tcggggcgca gacccaggct ttttctgaaa
     1801 aacgccccat cgccaaacag ttccaaaaac gtcggcaagc gaacaaacct gcccgagctt
     1861 gcgcggagca aaagccacgg acgcaaaagc acccgcgcag cggcccgcgg cgacggcaga
     1921 accgacacat cggtgggcat gctttcggtt tcccggtcgc ccaagctttg gtactgcgag
     1981 cgcacaatca gcaggtgcag ccccggttca aagcgcagcc gtcggtcggc cagcaagagc
     2041 tctcccccga tggaaaatgc gcctcgcaag cggtcgctct gcgccgcttg gctgcgatcg
     2101 cagtcgagcc gaggcgcggg gcacaggtcc cgctgtcggt tctgttcgta gcgcacttcg
     2161 gccacggtct ggagctgact ttttttcaga tcctcggcgg gccctgtgta gatgcgcaaa
     2221 aacgctttgg cccccgcttg ctgcgacagc tgttcgctaa gcaccgtcgg cagcgccagc
     2281 aaatctttgg actgtgtccg atcgacgagc ccatggacat ccagatcgat ttgcacgcgc
     2341 ggggttggtg tccgaagccg cacgccacca tctaggagta cccgtccggt ctggagatcc
     2401 ggctgaccgg gctgcgacga aggctgtcca attccggcca caccttgtcg ccgcagaaga
     2461 ccgctgctgt gaacatacag actgccggcc cgcgtctctg cttcgatcct tgcatcgacg
     2521 ctgatctggg aaaaatcgtc gtttttgcgc cgcagctcgt caagctgttc gcgtccgtag
     2581 cgcagccctt cgttgaaaaa atagggaaag tctccgtcgg cggcttgata ggcggcactg
     2641 agcagaagcc gtggcgacgg tgctttgtag ccgacacttg cacgccgcag accgaacgat
     2701 ccgctgccca aagacagcca ggccgacggc ggaccttggc cacgtcgcgt cacaagattg
     2761 atggcaccgc ccaatgtctc gctgccaaat tccagcggcg cgacaccacg atagacttcg
     2821 acccgttcca gtccgtctag cggaagctgc gacagatcga ccgccgactg gctgccgcgc
     2881 gaaagaggaa caccgtcgac aaacaccgcg acttgaccgg gcgccgcacc gcgcagactg
     2941 gcgccgctcc actgcccaag accaccgctg cgccggacct gaacaccggg ctgaccgacg
     3001 atcagttcgc cgaccgaact cttggctgcc gacggggcac ttgcttgtcc gggcagtccc
     3061 agttcgatca ccgaaactgc cgctgcagag tcgctttttt gtaaaacaga gtcacccgag
     3121 acggtggtct gaaacggggt cgtctgcgcc cggcccgcac ccatgcggaa tgtcaggatt
     3181 tcgcaaaaca ggcacagcag cagcaggcaa aaaggacgtt tcgtgtgggt tgaccagtgc
     3241 aagcgaccct tcctcccgaa aggttttgcc gcgtgcaggc cggtctcctg gctccagagt
     3301 cttccgaaca gtgaaccctt cccagcctga tggccagtgg gtctgccggc attgccgtca
     3361 atgttcgtcg tccgttacag tcgcgggggc ggcgccagct tgctggactg gcttcccgaa
     3421 cacctgcgca cgtctttgtt tatttgattg cgaggcaggc tagcgatctt ggcggtcttc
     3481 tgtcaagcgg actcctaatc gttgggaatg cgctgggaat gcgctggatg gtgggcgcta
     3541 cagaagaccc gcgctctgta ccgccttttg ccaggccgcc agcttgcggg caaacgacca
     3601 gcttgcattg gccgggcttg tcgacggcaa cttgatgagc gggaagtgcg actgtcccaa
     3661 gctggcccgc acgaccttgg cgtaacggaa gctctctccc ccgttgtgcg cgataagctg
     3721 gagcttgggg cagcgcgctt gcagactggc aaaatcattg accaccgcat tccgaatcgc
     3781 acggtccaga ctcccttcgc gagagcagga tgcgtagaca tcccagagac cgactcctct
     3841 ttcttgcagc cagtcacagc gcgaagcgta gtcttttgcc atcggaatcg tcggccagat
     3901 cgcctgcatg atcggccaaa aatgattctg tggatgggcg taatattgct gggccgcaag
     3961 cgacgcggca cccggaaaac ttcccaagca gagtaacttg gttttttctg aaacaagcgg
     4021 tggaaaacag aagagtgttt ctgcttcgct cggcggagca gctgccaatt cgcggcaagg
     4081 cgtgcgtgac atgaacgcag cctaccttgg tttgtcccta aaagtggatt ccatcggggg
     4141 caagggcaat ccgcggcaac gactgtgtct gggccagccg cgatccgccg ctgatgcgaa
     4201 cggtggtgcg tgacaagacg atcatcgtga ttccgcccgc cagcatcgcg gtgccgatcc
     4261 cgagcatggt tccacctgcg accaggaatc cgctgctgct gctctggccc acccgcgcgt
     4321 cgtagtttgc gttagcttgg ccgattccaa tgcccgcgaa agtaattccc aggacggtga
     4381 cgatgccgcc aaacgtcgtc aggacgatgc cgcccgcgcg tggtccgctg tgccccgcat
     4441 cgacatccag tgtcacaagc tgggtctgcg gaatcacaaa cgagctcgat gcattcatgc
     4501 gcagaccgcg aatctggtaa agaccgcttg gatcgatggg aacaccgcac ggagtccggc
     4561 acacaggaga ccagacatcg tagtgggaca gatagcggtg atgccgaccc cagaccatcc
     4621 gggtgcctcc acctgtgacc atgaacagcg tcgcggcggg gtcgtttgat cgcagctcaa
     4681 cgatcggctg ctgcggatag cttggatact gcggctgcgg atacggctgc ggagcgtact
     4741 gctgcggata cgccgaagga ttctgctgcg gagcatactg ctgcggcgcg tactgctgcg
     4801 gcgcatactg ctgtggatag gcgttaggag tctgttgcgg atacgcaggc tgcggatacg
     4861 gctgtggcga cggctgcgtg gaaacactcg gtgggggcgg aagctgcgcc agcgcgggca
     4921 gcggacagag tgtcgcggtg agcgagagag tcgtaacaag cgaagtcaga cgcatgctga
     4981 ctatcgtagc aggaacgacg ccgctggggc gatgcgaccg ctctgttgtc tggattggtg
     5041 agtgtcttcg cggtgttggc gccgtcgatt acggcttgat gcgaccatcg cgctgcgcag
     5101 taactgggct tttgcgctgg atgtagtcga tcaccacgtc atttacgatc ttgccggtgt
     5161 ctttggcggg accgacctgc gcaaacaggt ctttgtatcc ggtggccaca ttggccatgt
     5221 agtccgttgc catcaccgtg tacgttgcgt ccattcggat cggctgctgg tcggcttcgg
     5281 ttgcgctctt gcgcacggtg acttgcgtgg cgcgattccc atcgattgtg aaacgcactc
     5341 cccccgtgac tccaaacaga tcacttcccc gtcgagtcgc cgccaaatcc agcactgctt
     5401 gaagctgcgc gccgctcatc tgaaacgtga ggatcttgtt tttgtagggg agcgcggtca
     5461 gatagtcttc gttgcggatc ggaccgggcg gaatcgaagc gcggaacgag cttgcggaag
     5521 acagcgcgaa ctgggcttgg gccgcatcgc gcacaatgtc catcacgaaa ttccccagcg
     5581 gagattcatt ctgatcgatt ccgtccaggt ctagttccgt cgctgcgctg ccgatttttt
     5641 caaacagctt ggcgtatttg ggatcgcctt ccagatcgat ctgcatgcgg tgggttgtgc
     5701 tggataccgt tgcatcctgc ggcagttggg aatccatccg gaccagcttt ccggtgactc
     5761 ctgccagctt gccggaacgg aacagaagct caacgtggct tagatacgaa aggtactggt
     5821 acgggctgat gaagtacgtc tgcgtgcccg gaatctgcgc gaacgtacct ttgtaatggg
     5881 aatgggttcc caagatcaca tcgattccgg gcactgcttg cgccatcgca aaatcggaat
     5941 cacggtcctg gtgtccgatg aagacaaccg cggcgacctg ttctttttcg cgaagctggg
     6001 tgacgatttc tttggccaca ggaatcgggt ccagaaactt cgctcccgtc ggtaaattgg
     6061 ttgatttgac cagccgaaca aagtcacttc cggacagcgc aaagatgccg attcggaccc
     6121 cgccgacatt ctttacgaca tagggaagtc cctggttatc gagcagccgc tggccgtttc
     6181 catccaccaa gttgccggac agaatctgga tgccgctctg tttctgacag ttttttaggg
     6241 catcccatcc gtagtcgaca tcgtgattcc caaacgccat caccgacacg tattcgccga
     6301 gccatttcca gtcggcacag ccgtcgtagt atttgtctga ccacgcgggt gttccggcat
     6361 tccacatgtc accacctgac agcgcgatca cgctctgatc tttggctcgc gcaccggcaa
     6421 tgtaggccag cgtccgcgcg agtccgccct gatctggctt gtgctcgctg taaaacggaa
     6481 tcgcatgcga gtggtaatcg gaaaagtgga tgagctgcac ccgcacttcg tcgggagaca
     6541 tcattccgtt cgccgtagga gtcgctgtgg gatgacatcc ggccacagca cagagcgtga
     6601 gagccaaggc ggcgagcgtg cggcagcgca gaagcacatg ggaaaagaca gtcgtgggct
     6661 tgcggtgtct catgaccgcg catcctacat cgtcagccgc aaataagtgc tgactttcac
     6721 ttgggttttt gcccaagata tcgtgttggc aaagtgggct cttctgctgg gtctctgctt
     6781 cggggtgcag accacagcgt atgcacaagc aagtgatgac gcgggtggca aagcggctgc
     6841 cgaggatcgc ttttcctcgc gcgtcgtcgc cgatcgcgtc aaagttgatg aaccagaagc
     6901 catcgttggt gagcccgtgc gcacgcagcc cggaaccctg ggcgatccgt tccgcgtggt
     6961 ctcgctcttg ccgggcgtgg tctctccgtt tccgggtctg cctttgtggt cgattcgggg
     7021 tgcaagtccg ggtacagcgg gcttttatct ggatggactt cgcttgccgc agctgtttca
     7081 tctgctgatt ggtggtggcg tggttcattc cgatctgatc gatcgggtcg atttttatcc
     7141 cagcggcttt gatgtcacga tgggaaaagc ggcgggcgga atcatatcgg ccagcacaag
     7201 acccgcacgc agcgacggaa atcacttcga tctgtctttg cgactgtacg atgttggggg
     7261 gcttgtcgag ctgtctcttc ccaaagaaat ccggatctct gtatcgggtc actacggcta
     7321 tccgggcccg attctgcgcg cgatagacaa tcgaattcag cttgattatt gggactatca
     7381 agtacggctg gactggcgat tcctgacgtt ccaggtgctc ggcggatatg acgcgctggc
     7441 gctgtctttg ggaaaggaac tggagtattc ctcgccgcag agtacgcgca cgatgtttca
     7501 tcgcgcccag atccgtttgc atggggaaag cggaaaagtc agcggcgaag tggcgctgtt
     7561 tggcggctac gatgaagcgg gagacatttc cgatcgcggc gtcaacaaac tgatggcggg
     7621 cgcgcgtggt tttgtgcgtg tgcggctgcc gcgtctttcg ttccaaatcg gaaccgagtt
     7681 agaagtcgcg cgcttttccg gagagcgctt cgatgtcggt ctgcgctcgc tcgcgtttgt
     7741 tcgcaatgcg gaagggccgg cgcgggtgag cggaaaagat cccgatgcca caccagatga
     7801 gcttggcgaa atcggcaaca accgggttgg gattacgtcg agtttttacc ttcacagtga
     7861 gctttcgctg tggccgcagc gactgtatct gaccgcgggc ggacgggtcg acatctacca
     7921 tgttggcgat gaaaccctga tcggttttga tccgcgtgcg cagcttcgtt ttgttgcgac
     7981 accttggctt tcgttgcaag catcgatcgg tgtgtatcag cagccgccgt cttttccggt
     8041 cttgcttccg ggggtggata ccttcgctct caagctggga cttcagcgcg cgcagtcgac
     8101 ggtgattgcc gaagagatga gcctgccgtg gaacctgtca ctgcggctta ccggatttca
     8161 tcagcgcttt tccaacatga cggatctgcc gccacttggt agctcggtct gtgcatcacc
     8221 gccaccggaa aagctgcacg gaacgactgc aacccttgtg cgggtggcgg acggacaagc
     8281 ttacggaatg gaggtctggc tccgtcgcag tgtcggtctt ctcaccggat ggatttccta
     8341 cacgctgagc cgttcagagc gttcctatcc ctgtggtgtc cgtcccgcgg attacgatca
     8401 aacgcatgtg ctgcaagtgg tggcgcagac ccggcttccg cgcggttttg tcgcaggtgt
     8461 caggctccag ttttcgaccg gacgacccga aacgctcgtc gatgtgggcg cagacaatcc
     8521 atctttggaa cagattgcag agcggacagg cgggcagggg gttcgcaaca acttccggct
     8581 gccaaactac gtccagcttg atgtgcgaat cgacaagcgc tggacgttcc gccgctttta
     8641 tcttcatgca tttttggaag tggtaaacgc tacgttttcg cgcacgaatc tgctcctggc
     8701 ctatccttct cccgacgaac aagccaatgg gaagcacgat ccggaactga ttggttttag
     8761 ctggattttg ccgtcgatcg gtctgcgtgg cggtttctag atcgactgtt gaagcagaca
     8821 aaagcagtcg ttagaaaaac gaaacttcgc tggaagtcgg cttcgctacg ctgcttgtga
     8881 gaacacacga tgtaccatct gctggcccgc cttcgcgtgc tgcatgaagc gctgacgccg
     8941 gaggtggatc gttttctacc gcctgatctg tcgccaaact acaccccatg cgtgtccagt
     9001 cagcatgtat tttgcggtcc agaaggcagg ctgatctcga tcggaatggt gcgaagtgac
     9061 gactccggaa cgattgctct gcgactggtt cgcgagcgaa cggacggtgg tttggacgga
     9121 ctttccctgg tcgcgccgca tgcgctgggt gcatcttttc agcaagatgg actgctcccg
     9181 ggtcgcatgt ctacgaatcg gctccttttg tcgcgcgggg aacacgcgat tgccgggatg
     9241 tatcaaagtg aagaccagac caaaccgatt cactctgttc gcttttcact gacgctaaaa
     9301 ccgctggaaa gaggcgtcgg aatcggccag ttaggactaa ggctcggaca cctgatcgcg
     9361 acggattttc tgcgcgtttt gtaccgcgga gagctgttcc taaacggtga aaaattacag
     9421 attgatagcg ttggatcgct gtcacttcac gcgggggacc ggctgccgca gtatggttat
     9481 ttggtcacga gtccgcatgc ggcaagtgga cttctgccgg cgcttctttt gctcgccgtg
     9541 cacgacgatt cgctgcgggt gctgggtgaa tccgtgggtg gacagagtgt gatttacgga
     9601 ctgggccgca acggtcttcc gaaagtcgca ttgcacctgg ggtcgcttgg gccggcgctt
     9661 ccgctcgggg atgatgggaa gctgcttttt cacgatctga gaccgtttgg ttatgacttt
     9721 ttgggacagc ccactgtgac ggcgctgggg gatgcgcgct ttgtttccca acaagggact
     9781 gtgcgagacc tggggcgggt gtttgtcgac tgtcgcggcg cacactttgt gacgcttttg
     9841 cattcctgaa ctgcgcagtc tgccattttg ccagatgatc cccggatttt ttctgtgcta
     9901 gggtgccgcc atgatcgatc acatctcttt ggcggtttca aacctcgacg ctagcgttcg
     9961 cttttatgaa aaggcactcg cccccttggg atacgctgtc ctgctgtctt ttccgggtgt
    10021 tgcaggaatg ggaatcgctc ccaaaccgga cttctggttg ggacaaaagg aaagcgcgga
    10081 ctcggggcgt cacattgcgt tttgtgcgac aagtcgcgcg caggtcgatg cattctatca
    10141 agcagcgatg gcagcgggag ccaaggacaa cggcggaccc ggacttcggc cacagtatca
    10201 tccaaactac tatggcgcgt ttgtgctcga tcccgatggc aacaacatcg aagcgtgctg
    10261 ccacggtccc gcataggggg gcttaggaat cggccttttc gatccgtcca tcgacgtgct
    10321 tggcgaagcg accggcatcg cgcggatgga ctggatcacg gcgtttccat ggccagccgc
    10381 caaactgcgt gcgctggtag tctgcaaatg cttgccttat gtcggcctgc gagttcatca
    10441 caaacggacc atgctgcacg acgggttctc ccagcggtcg gccttgcaga atcaatagct
    10501 cgctttccgc ttcgccgttt tgaatcggcg cttccacatc ggcgcgaatt tcgaccagat
    10561 gccgcgccgt gatcggatgc ccacacagcg aaagcgacgg tccagaatag aaatacaccg
    10621 tgcgacggac tcctgcagat gctgccggaa tcgaccactt tgcaccgggg gccagcttga
    10681 ccgtccagat tgcgacttca ctttctctgc ggctggcata ggagtgcggc gggggcggaa
    10741 gtggcttctg ggaagcaagc gcgcctgcga cgaccagaac atctactccg ggggttagca
    10801 catgcggaat gtggttggcc cacagcatgg aaaagtgcgg atcgaccatc ttgtctgcgc
    10861 gcggcagatt cagccaaatc tgaaacagat cgatgggatt tctggcattc cgatccaaaa
    10921 gcggaaacat ctcggcgtgc tggattcctt ttcccgccgt cagccactgc acatcgccac
    10981 gaccgtaacg cgcggtggct cccagggaat ccgagtgatc gacaaagccg cgacggacaa
    11041 ttgtgacagt ttcaaagccg cggtgtggat ggtttgggaa tcccggcacc acatcgccgt
    11101 gatacatgcg aaagccgtct tttcctgcaa aatcctggcc gatgtcacgg cctgccagcg
    11161 acgctgtggg ccccatctgt tcgttggcct gcggatagtc atcgttgtga tggacgcaga
    11221 acagaaacgg atcaacggta tcccaagtga atcccaacgg gcggacagaa agaatcgcac
    11281 tcatgttctc actatagcgc agcgcttacc gaatgttctg ttcaaagcgc gcttccccaa
    11341 gcgcaggcca ctgtggcatt gccagcgtgc gcagcgtctt gtccattgct tccagtctgg
    11401 cggtgggact tggatgggtt tgcaaaagca gcgcgagccg actgcttgaa cccaggatgg
    11461 attccagctt ctgcatcacc gccaaaaatg cataggggtc ataccctgcg cgggacatca
    11521 gtcgcagcgc ttcttcgtcg gatgcaaact catcgcgctt gtccagaccg cggacgtaaa
    11581 gctgcttggc agatgacaca actttttgtg caaactctcg cgtcccgctt ccgaagcgcg
    11641 aaagaggtgt gttgtttccg accatggaac cgacatcggc agcggctccc atcatgtttt
    11701 ctttgcgcat cgcttccaag tgatgctgcg cagtaacgtg cgtgatttcg tgcgccaaaa
    11761 cccccgcaag ctctgcttca ttcgtcagca gatcgagcaa accgcgcgtg acaaagacat
    11821 atccacccgg agccgcaaac gcattgatgt ctgcgtccgc caagatacca aagtgccagt
    11881 ccagctgcgg tcgcgcggtc gacagagtca gccagcgccc cacctgattc acatagcgct
    11941 gtgccgccgg attctgttcc agcggagaag cacccagcag aagtgccgcc atgtgtcttc
    12001 ccatctcgac ttcttgatct tcgggaaccg gctggtttgc cgcttcgatt ttctgtccgg
    12061 cactttgcag cgcgcttagg tcgatctgtc ccgacgaaaa accaccaagc tgcaaacagc
    12121 cgctgagcgc tgcgaccaag ccaattccaa gcagtccctg tctgggtttt gtccggaaac
    12181 gcatcggctt atcgcattcc tttctgcggg acggcctgcg ccgcttcgct tgccggactt
    12241 tggacagctt gtgcgttcgc cgctgcgcca aactgtcgcg cttcttccag cgtgacgcga
    12301 taccgttcca gctcggcaac tgccgccgga ttttcgtttg ctcgctcgat gtcggtgcgt
    12361 gacagtccgc ggattccggt cgcaacggtg gtcgagctgc gcgcggcagg gctcagcgca
    12421 atcacgccgc tgggtggcgg ttcgcttgtc cgaaccacgc tggtcaaacg cacagtcagc
    12481 atccggacaa agccttcacc cgccgcggtc ttgacctgat aaaacgcgcc ctgtcgtccg
    12541 ccaatgtcca ctgcggttcc tgctgcaagc tgggcgaccg cgggcgcact tgcttcggct
    12601 ttggcgcgca gttcggtttt ggttacaagc tgtccgctgt ctgcgcacag catcgcaggc
    12661 catgcagcag tcagcgacgc aaggcacagc gcgggaaagg ccgctttggg aaagtgggtc
    12721 acgcttgttt tctccttggt tcatagacca ggacgggctc ttctcggcct ttgactttgt
    12781 gggctccgtg cgcgacaaaa agcagcgcat cgccgcacag ctcgcgtgtc ttgtgggaaa
    12841 ccagaatgcg cgcgattcct ttggtcgttc cttcgagccg gctcgccaga tttacggtgt
    12901 caccaattgc ggtgtattcc atgcgctgct gcgatccgag cattcctacg actgccggtc
    12961 cggagtgaat tccgattccg acatcgaagc cgggcaggtt gcgctctttg cggaatgcat
    13021 ccagttcatc caccatatcg agcgccgctt ccaccgcatg cagggcgtgc tgggggtcgg
    13081 caaccggagc cccccaaaac gccatgatcg cgtcgccaat gaacttgtcg agcgttccgt
    13141 gatgacgaaa aatcacaccg acttgccgcg taaagtaagc gtttagcagc ttcatgattt
    13201 cggcggcttc tgcggtttcg gacatgctgg taaagccgcg aatgtcggaa aacagaaccg
    13261 tgatgtcaca ctgtctgccg tcgagtgttt ctggtcgcag cccttgttcc gccagctctg
    13321 ccaccaccac cggatcgaga aaacgaccaa acagatcgac tgtggcttgc cgcgcttttc
    13381 gttcttgcca gtaccgatcc gccgtcgcta ccactaacag caccatcatc gataggatcg
    13441 gcgtcaggac cggaatcaag atccgcagcc gcatcgcaaa aacacttgcg aacacgagca
    13501 aaccgccgag cagcacgcca aacagagccg tcttggtgag cgattttttt ttcgccagtg
    13561 ccgcatacag cgccagcaaa aaaggcagtc ccactgccgg cccataccag ctttcatgac
    13621 gcagccaggt gcgatttttg acgttgtcat aagcgttggc caaaagatac agcgccgggt
    13681 attgcgcccc gatgggagtc ggtcgcagat cgtgcagccc cgacgctgtg gaaccaacaa
    13741 acaccgtctt tccggccaga tcctttcgct gttcgccttt ttgcacttgc agccacagat
    13801 cggcgtagct cagcttgcga tagggggact ctcctttttg tccgataaac gaaagccgca
    13861 gcttgtccgg aattccctcc accgcgaatc cctgcgcctg cgcgagcacg agcggcagcg
    13921 acgcaatccg ccagccagga tgtggttcgg cagtcagtcc gtagtgtctg ccgattccgt
    13981 ccgagtccgc cagatagttg ataagcccga gccgccagtg cgccggattt cccatcgctg
    14041 gcagcagcag atcgagcttg gcctgtgggt cggcatctct gctgcgcgtg atcggcaggc
    14101 tgggtggata gctcgacaaa agccgcaggg ctcggtctga cttgagcgcc aagagcggca
    14161 gaaacagatg gctcgatcgg tcgatcactt ccccgaaata cgtgtcgaac tccggtcgct
    14221 gcagatccgg ttcgctaaac cagatgtcga gcgcaatcgt gcgcacgttt tgcgccgtga
    14281 gccactcgat cagctcggcg tgggtcgcgc gcggccacgg ccaacgtccg acgctgtcgc
    14341 gcatcgcttg caggctgtag tcgtcaatgt cgacgatcac aatgtcgggg tcggcgtgcg
    14401 cgcgggcggc ggcctgcctt gtcagacgat ccagccacag aaagtcaacg tgccgcgccg
    14461 acgaaaaaaa caggggcaag accagcagaa gctgggccag cacaagcgca agtcgcagcg
    14521 agatggcaga ggcaaaaggg ggccgcatgg gcagcgtcgg atgcaccgac acaaacatcg
    14581 atcgtagaaa gaaaatcccc cttctgcaag cctaggcggg gcaagacgtg tcgtcggcgg
    14641 ggcgcagacg gcctgggtta gcagtgaccg aaccagccgg tgggaccggg atcgcctctg
    14701 cgtcggcgac gtggcgtgcc gcggactttg ctggtcacaa gcgcaagcag agcgagcagc
    14761 gcgctggcaa aacctcgtcg ggacagagca cgttgcagac tggacagcga ctgtaggccg
    14821 attgagcgca tggcactttt cccattatac agcaggcagc ggctcgcagc cgatcttcgc
    14881 ccacatctcg tcggcagaca tccctttttg ccgcagcgat cgaatcgact cggagccgcg
    14941 ccgcttggcc aagcgctcgc cagccacgtc gcgcaacagc gggtgatgtg catacctcgg
    15001 cgtcggcagg tcgagcagcg cttgcagctg ccgatgcaca tgtgtcgaca aaaacaggtc
    15061 ttcgccgcgc gtcaccagcg tgacttgttg gatcgcatcg tcgacgacca ccgccaggtg
    15121 atagcttgtt cgcacttctt tgcgcgccag caccacgtcg ccgaccagac ttggacttac
    15181 tgcgacggtt ccgtgatgtg tgtcgaaaaa atccagcggc cagcgtccca agtcgcgcag
    15241 ccggcgttcg gttttttccg tcgacaaacg cagcgcatag ttgtgccctt gcgacatcct
    15301 cgccgccgct tcctctggca caagcgaccg acagcgacca ggatagatcg gtgccgcctg
    15361 ggctccgtct tggggcgcac tctgcgcggc ttgcagctcg gctgcgatct cggcgcgggt
    15421 acaaaaacac ggataaagta cgtcgagagc gagcagtttc tgaatcgccg cttcatagtc
    15481 ggccatgtga tcggactgct tgcgcacagg ggtcggaaag cgcagaccta aaaagtgcat
    15541 gtcttccaaa atcccgtcga caaactcagg tcgacagcgg gtttggtcga tgtcttcgat
    15601 ccgcaaaaga aacgtgcccc cgtggcggcg ggccgcatca aagcccatcc gcgccgaata
    15661 cgcatgtccc aagtggagat agcccgtcgg actgggcgca aaccgtgtca ccatcttggc
    15721 caccatagca cgccgaaaaa cagccgacag cgcgcggcga tcatcgtatc tgcgctgcgg
    15781 ttaggatcat aaagtgacca acccgcctgc acccgtcagg atacgtttcc gtccgtggct
    15841 tttggctgcg gtggcggtgc tgtggacggt cattctgctt gggtttctgt ttcgctttgc
    15901 cgagctgccc agtctgacgg cgtatttggg tcggctgtgg cgcggtcctt tggtgcagct
    15961 tcagattcgc gcggtgtgga tgggcttcca tcttgtcgga ctgggactct gttcgtttct
    16021 tttcgcgcgc cgactcggtg tttctgcgac ggatcttgga ctttccattt ctgcgctgcg
    16081 aaacgaacgg aatcgcattg tgtggatcac ctgtggctgt cttcccatcg tgcttgcgct
    16141 gtcttttgtg ccggcgtttc agcgcgctta tccgatgttt cgttcgtcgg aaagcggggt
    16201 ctttgtcacc cttttgtggt tgggaatcta tgcgctgtat ctgttttcga tagagctgta
    16261 ctttcgcggt ttccttctcg cgctgctctc accgctttgg ggcggtttgg cagtcgggat
    16321 cgccatgatt ccctatgtgc tgacgcatcg atttggtcct gaaattgtcg gatcggtgcc
    16381 ggtgggaatt ctgcttggga tctggcggca gcgctgcgga tcgatcatcc ccggactgct
    16441 gacccatctg ctgatcgcga cggaaatcga gctgtgcggn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnt catccccgga ctgctgaccc atctgctgat cgcgacggaa
    16621 atcgagctgt gcggactgtg gcagcttcac agatagcaac cgacagaaca gagagcgaac
    16681 agagagcgaa cagagagcga acagagagcg aacagagagc gaacagagaa cgaacagaga
    16741 gcgaacagac aacaagcaga ctcggacaaa aacggataca gaagggagtc ccaacatgaa
    16801 gacgcaccgc ctggatttgg catttctgcg cattttgctg gtcgcggttc catttggctg
    16861 cggccccaaa aaattcaccg aaccggcacc cacggggagc tgcgaggcca accagatagt
    16921 gcagttgtcg gacatcgcgg cccgtgacgg tggcagtggc ggagacgggg gcgtgcgatt
    16981 ttcgggcgac ggcggggtca gcgtcgccaa cacgctgtcc gatgccgact gcatccagat
    17041 ttgtaccagc acatcgacgt gccagccgat cgttaacgat ggaggaactg tcaccagcgt
    17101 cgagtgcatt tatccctgtt acgcttttgg ctgcggtcgt cggccccagt cgctgccact
    17161 ggttcggtcc ggacatccgt cggcgctggt ccgctatttg gaagaagcgg cctacctgga
    17221 agccgcatcg gtgtttgcgt tccgtcgcct ggcgcgcgag cttttggccc acgatgcacc
    17281 gtcgtggctt gttgcgtggg cggatcgtgc agcaaacgaa gaagtccgcc atgcgcgggt
    17341 gatggctcgt ctttgtcgtc gctacggtgg gactttgaca atgccggtgg aaggtccgca
    17401 aggtgtgcgt ccgctggctg aaatcgcgcg ggaaaatgcg caagagggct gcgtgcgtga
    17461 aacctatggg gcgctgctgt tgtggattca gtcggcttct gcaacgcaac cgcatctaca
    17521 gcgggtgttg caggtgattg cccgcgaaga aaccgaacat gccaagctgg cgttttctgt
    17581 ggcggacttc tgtgcgtcgc gtctgtcgga tgcagagcgg gccgaagtcg accgcgcaca
    17641 gcaagcggcg gtggcggacc tgggcgaagc gattgcgcaa aatccgcctg ttgaactgca
    17701 gcagcgactc gggttgccgc ctgtcgagct ggcgcagcag ctgtaccaat cggccagccg
    17761 tgagctgtgg acaatgaccg ctacagcttg ctgacgttgc tgcttaccag tgcgaacggc
    17821 atcgcggaaa cgctggacac tttctgatcg gaagcgaaca ccgtcttggg attggtcgct
    17881 ttttctccgt cgatttgcag cgaaaacacc acgggctgct cggttgcgcg gaactccagg
    17941 tgcaccggcg tgttcgcttc cgtctttgca tcaaacgtaa agtggtaggg cgtcagcacc
    18001 gtcacggagc cgcgcggact ggacttgaaa tagatggtcg actgcacgca ggtctgcgga
    18061 caaaacactt cgccggaaaa gcgtgcgctc gactctgttt gccattccaa ataccagcca
    18121 tttccgtcgg tggtgatccc aaacgcgcca tagggtacgt cgaccgatgc gttggcgctg
    18181 ataaggaatg cgggcgttcc ttgcgggact tccggaggca gccgcagcgg gctcgaattg
    18241 ctggggacaa agctctgggc ggtggggtaa ttgccacagc caaaaagaag gcatgcgcac
    18301 aacagcagcc gctgcatcgt gaactccttc cattcaggct atcacgagcg gtggatgcgg
    18361 gctttagcga cgggaattgc cagccaagac cggacgccaa ctggccaaaa acgaaggcag
    18421 atcgcccagc gcaaagcgaa cttccgcctg gccggtttcc cgtccgtgcg cgtcgtacag
    18481 atacgccggc agaaccgtca gcgtcgtcac tggatcgagc ggcgagatgt ctttttgtcc
    18541 agcaaaccga tacggcgagc cgtcgcttgc ggcaaaaccg aactcgtagc gaatctgcct
    18601 ttgcagcggc aaaatccaca gcgaaccgct gagtgccgcc gagtccgcaa agccggaaat
    18661 cgacacggtg ccggcgatgt cggtgcggcc ttcttcgagg tattcaccaa gcgatggcac
    18721 ttcggcgtgg atgcgaaact ccatgtgccg cgggtgctga ctgccatcga gcgtgatggg
    18781 tcctgccatc gtctcgtcga acgagaagcc aggcagtttt tgaagatagc tttgggcctc
    18841 ggtttttagc tgaaacgcga cggccatcat ctgctgggct ttgtcgagta gcgatgcgat
    18901 gttcataccg cctgtgtagc acagccgcag ccgcttcgcc cgccgcgcga tgctacggca
    18961 aaagtcgctg cagagtcgcc gcgcgatcgg ggggaagcgg ggctgtcacc gaaatgcgtc
    19021 ggctgggatc agacggcgag acaaagcgca gcaggctggc atgcaggaac tgtccaccgg
    19081 gttcgccttg tccgtagagc ggatcaccga cgatgggcag acccaagtgg gccaggtgaa
    19141 cgcggatctg atgccgcctt ccggttcggg cttggacccg caaaagggtg tgcatggggc
    19201 cgcgccgctc gacgaaaaaa gcggtgtgcg cgtcgagcgc atcggggtga taaatctctt
    19261 cgggcgtcgt cgcgacgtgc attttgggac tgccgcctcg accagcgagc agaggcagcg
    19321 tcacatcgaa ctcatcgtca tcgggactgc cggccaccag cgcgaggtat tctttttcga
    19381 ccagtccacc ggcaaagtcg gcgcggagct tggtccaggc cgcaggggtc ttggcaaaca
    19441 gcagtgcgcc acttgtgtcc gcgtcgagcc gatgac
//
LOCUS       DAORGN010000081        19130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1138_length_19130_cov_5.301861, whole genome shotgun sequence.
ACCESSION   DAORGN010000081 DAORGN010000000
VERSION     DAORGN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1138_length_19130_cov_5.301861"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKL17_10740"
     CDS             complement(<1..517)
                     /locus_tag="PKL17_10740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45252.1"
                     /translation="MDQAETPAAAGPETGEPAKRLLGTTVGGRYKVVSLLGEGGMGAV
                     YLVEHALMRKRMALKVLNSELSKNREMVARFEREAMAAAHLEHPNVVAATDLGTTDEG
                     SLFLVLEFIDGKSLRDQLNFGPLSPARVIHIARQVASALQRAHSVGIVHRDLKPENIM
                     IMNRDGDPDFVK"
     gene            complement(599..1756)
                     /gene="moeB"
                     /locus_tag="PKL17_10745"
     CDS             complement(599..1756)
                     /gene="moeB"
                     /locus_tag="PKL17_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNK45253.1"
                     /translation="MPNYQEYLRQIRTNVQELSVEELRAERLATANELLVDVREPDEH
                     LQGVIPGAVLIPRGLLEGKIESVAPDPNAHIVLYCQGGHRSALAASSLVAMGYGHVRS
                     LAGGIAAWRTAGGPIEKPQVFSAEQRSRYSRHILLPEVGEKGQATLLKSRVLLLGAGG
                     LGSPSAYYLAAAGVGTLGIIDSDVVEESNLQRQILHNTRRVGRPKVESAAETITQLNP
                     DVKIKSHNVRLTEHNAVELLSGYDLIVDGADNFPTRYLLNDASVMLRIPVIHASIYRF
                     EGQLTTFLPFVGPCYRCLYPEPPPPELAPSCQEAGVLGVLPGVVGVLQATEAIKLLLG
                     LGDSLVGRLLLYDALAATLREVKLRRDPDCPVCSDESRPIQLVDYKAFCAR"
     gene            2006..2398
                     /gene="erpA"
                     /locus_tag="PKL17_10750"
     CDS             2006..2398
                     /gene="erpA"
                     /locus_tag="PKL17_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNK45254.1"
                     /translation="MTTNIRKPDAAPEPVPAPVAATSPISLTQVAADKVMEIRKDEAI
                     EDTMFLRVRIVGGGCSGFSYDLYFDTSTPMDKQLVSNGVTIIVDQMSLQYLRGTEIDY
                     VEGLQGAGFKFHNPNVKSTCGCGSSFSV"
     gene            complement(2447..2833)
                     /locus_tag="PKL17_10755"
     CDS             complement(2447..2833)
                     /locus_tag="PKL17_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45255.1"
                     /translation="MQDLRLYFPGQLPWLAQAVEETGLTGPLTYDSESLFEWCEGEAL
                     ELPVRINLTRAQRDGEMDIEAPVYVRMFTRFDEPVEPDALTAFVEKLAEKLSDTAHTD
                     IDIGAMSYEGGEGFSFAVELSLPGNK"
     gene            complement(2906..4102)
                     /locus_tag="PKL17_10760"
     CDS             complement(2906..4102)
                     /locus_tag="PKL17_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNK45256.1"
                     /translation="MTQTTDAALSTGTLADVISRKRIVMVVGSGGVGKTTTSATLGLL
                     AALRGRRVLVLTIDPARRLADALSVSALSHEIQKVPEERLRILAAARGETPVPGGVLD
                     AMMLDQKRAFDDLVARYATDPKLRDRVLRNQIYQQISSALAGSHEYAAMSRLYELSQL
                     DRYDLLVMDTPPTENAIDFLDAPDKMAKAVDSETIQWIMKQYLQQAGSLSLRLLGMGG
                     TIVLKGLGRFLGSAFLTQVAEFFVEFQQVLAGFRERAQKVTELMQKPEVAFLLACSPE
                     PLSIDEAVYFHERLTRAKLSIGGCIVNRVHSHAREVPADLLPILQTRPELSGFASDDL
                     AALADELSRTYAEQRTLAAADEASIRRLGEKLKLPLSQIPLLEQDVHDAASMAAVSRH
                     LIPPAR"
     gene            complement(4126..5472)
                     /locus_tag="PKL17_10765"
     CDS             complement(4126..5472)
                     /locus_tag="PKL17_10765"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK45257.1"
                     /translation="MTAHTGKSGFPRYVVIGGGVTGCVLAERLKQRRPQSSVTLVEGS
                     STLGGLSVRSEPDPIGWDRFYHVVTPQDVMLTDWLRRLGCDGKLRWRGAHTGFYADGE
                     LHAFDTPIDFLRFPPLNLIEKSRLAASILYASRVSDGTPLWNVTAKQWLTQIAGEAVY
                     RKFWQPLLRAKLGSAHEEVAATFIWSTIRRLYATRQGESRREAFGYISGGYAPVFSAA
                     EQYLQRLGVTVHKSAPVSELVDNSDGSTCIRTASGQELFADRVIYTGPSRFLPKLCAR
                     FPVPSDLLHRAEQTRYLGVLCLVVKLLRPLLPYYILNLTDAGMPFTGVIGLTTLVDPS
                     ETGGHHLVYLPRYLPDDDPDFDKPDSAFTEPFLRALTQIARHRGFRLDEVLSTHLFRS
                     RFVSPIAVRNYDRLRLPIPLVPGRLYLLNTGRILGGTLNNSQMIEEAERGLDEILA"
     gene            5549..7831
                     /locus_tag="PKL17_10770"
     CDS             5549..7831
                     /locus_tag="PKL17_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HNK45258.1"
                     /translation="MNIVCLGGGPAGLYLAILMKKADPSHRITVVERNRPADTFGFGV
                     VFSDQTLSHLRDEDAESYDAIERAFYHWDNIDVFYRDQVIRSCGHGFAGLGRHDLLRI
                     LQKRALELGVEIQFEREIQSGDVDSYLDADLVVGSDGLNSVVRARFADTFAPQIDWRP
                     NRFVWLGSTQPLPAFTFYFDENEHGLWRVHAYRYTQDAAEGEPQSTFIVECTAETFLR
                     SGLDPADEDATLQYCEKLFAPRLAGHKLCKNRSLWRNFPTVRCGKWSHKNVVLLGDAV
                     HTAHFSIGSGTKLAMEDVIALCKALREGGRDIRQALQAYEAERRPVVESLQRTAQTSL
                     EWFEHSERFVDCEPWQFGFSLLTRSLRLTHENLRQRDPNFIAEVNDHVAAQAERQTGV
                     KVPRHPSPPPMFTPLRVRDLIIPNRVAVSPMCQYSAEDGMPSEWHLVHYGSRAIGGAG
                     LVISEMTDVCREGRITPGCAGLYKPEHVPAWRRIVDFVHKHSEAKMGVQLAHAGRKAA
                     TCLPWVREDEPLPEQESWPIMGPSAIAWNERSQIPREMTQKDMESVLADFVAATEMAN
                     EAGFDLLELHMAHGYLLSSFISPLSNLRQDEYGGSLKNRMRFPLEVFAAVRAAWPDEK
                     PISVRVSAHDWAPGGLLPGDAVEVARMLKAAGADIIDVSSGGTAPEGRPQYGRLYQTP
                     FAEEIRLGVGIATMAVGNISSYSDVNSVIAAGRADLALLARAHLFDPYWTRHAAADQG
                     YQMKWPDPYLTVANYRPRFK"
     gene            7881..8747
                     /locus_tag="PKL17_10775"
     CDS             7881..8747
                     /locus_tag="PKL17_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNK45259.1"
                     /translation="MKNAVLFRSTFALCLPLATLAMSPRLASAQQLELPRPSPLAKVS
                     QMVGLTEVAIEYSSPQAKGRKVYGGVVPLDKLWRTGANAATKLTFSKDVTVDGKPVAA
                     GSYALFTIPGKDTWTVILNKNPNQGGTGQYKQDLDLLRLTVKPEAVPHRERLTFLFAD
                     TTENSTRIDLEWEKLRVSIPVKAATETQVDAGIKALEENSWRPYNNAARYLLESKKDP
                     ERALQLVDRSLSLHEDWLNNWTKAQLVGGKGKTQEACQLAQKAKTMGEKNPDGFFFAD
                     EVKKAIADWKCR"
     gene            8920..10437
                     /locus_tag="PKL17_10780"
     CDS             8920..10437
                     /locus_tag="PKL17_10780"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNK45260.1"
                     /translation="MDTGSTPPTADFESAFPSLIDCEPSDSDVDRWQREARELLRLAM
                     ERTSRIVKQLGPSRQGLRTHLANLQLAYDYLADLNEESDDGRTPSKSLNLGLSQRQDI
                     AKMVRDRRMSLGLSVLALAQRVGLSEGTIKNLESPRKPITRTTLLRLAAAAELGLQFD
                     DILFDQPSPDQGEPMNCFIAPGFNPVQMVIELGEKLNSVGGLIEQTNVYLDHQSAAAY
                     LKLANQLDYVAAFRDPVPMQKIADVILESTGRAGIDVIALGSGDAREEVRLVQHLIAA
                     SHPPDLRLYLLDVSQPLLLTGFIHAAETLADQYGVAYFAMQGNFHHLPRYTQLNYTPT
                     RSHRRRVATIFGGTMANLEQEGQFFRHSLSGLAPGDIAVVYAQIAVAPPNQPDVILRL
                     EPALQKPMSAAHKNWLSDPIYRHCKEVTDVELHLELNTSCPVPGSYALEAIATVKLTG
                     RRQKVFSMHRWRKYDPQLLSEHLKTMGWDTLALLPSGSSPKNQASVLVLRKRNTA"
     gene            10568..11257
                     /locus_tag="PKL17_10785"
     CDS             10568..11257
                     /locus_tag="PKL17_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNK45261.1"
                     /translation="MRKNNLTAKLAMGLYAMVLGTAEPSAKAASPRLPQDALPIPLVR
                     QQSDHSCGPAVLVAVLRYFQVWDGPETNLHRLLGTSADQGTLPEAIAFGARRFGLQAS
                     VEQPMTLVQLQSAVRAGKPVILELQAWRDPKGRRESWLNNWDDGHYVVLTGMDHQYAY
                     FMDPSTDNAYTYVPLQELLSRWHDVNPVRGPRSQHDYHLGIVISAPASIARHRGITRR
                     SKIIDRLIKLD"
     gene            complement(11271..12392)
                     /locus_tag="PKL17_10790"
     CDS             complement(11271..12392)
                     /locus_tag="PKL17_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNK45262.1"
                     /translation="MNRLVETIPPSAIRMLNDRKRADAIDLGMGQPQLHPDMAPLEAA
                     LKWIREHGCPYSQNAGFVELRAAIAQHFAYPGLRSPEQVCITIGSQEALYLPIKALLD
                     PSLHEALVVTPTFPAYQKLCHIEGIRVVEVQLDPKKDFAPDAAAILSQITDRTRLVAI
                     ASPCNPTGRVWPESELRKLSDGLLALPQPPYLLSDEVYAELYFTEQRPPSPAAFYPHT
                     IVASSLSKSCALTGLRLGWIMAPQAAAQVMWKAHQFVVSCADTFAQRAAIEIFRQPEL
                     LSKHRPHYLSQRQVLLDALAEEGLSAVTPEGSFYCLIRLPDAWAHRTMDAAVKIMEEQ
                     NVVTIPSSIFGAEGFLRVTFVARPELLREGIRRIKRALS"
     gene            complement(12420..13517)
                     /gene="dapE"
                     /locus_tag="PKL17_10795"
     CDS             complement(12420..13517)
                     /gene="dapE"
                     /locus_tag="PKL17_10795"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNK45263.1"
                     /translation="MMQTPLSDDALCRWLAEETLALCAIPSVTGDEAQITSHLSDRVR
                     SLQPHAEQKRIGNALLVRCGKPNGPRIGLFGHSDTVPPNSAQPHHIDWQSGRIYGLGA
                     SDMKSGLAVMLRLIADAESLPCELVCVFYDREEGPVHLSGMVPLCEAEGDFLRTLTAA
                     VCLEPTSNRIEAGCSGSLQAQVFAKGKRAHSARPWLGENAIYQVTPLLQKLAGWQRRA
                     VDKLGLTFYEVMSATQILTENARNVIPDRVMVNVNYRFCPGKTLDTAEAELREFVGPG
                     FDVHLVDASPSGAVCTDHPIVSSWIKQSGAAVTAKQAWTDVARLTGLGIAAVNCGPGD
                     PEQAHKVGEWCLLAGPLENYKLLGQLLRQRG"
     gene            complement(13521..14348)
                     /locus_tag="PKL17_10800"
     CDS             complement(13521..14348)
                     /locus_tag="PKL17_10800"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNK45264.1"
                     /translation="MHSTTSNRQNTISAAFSDRGLLKDPDVQQAIFATIEDLDKGRIR
                     VAEPGENGWRVNAWVKQAVLLYFVLRPLEPMDAGALSFFDKVPTKQNLKEAGIRVVPP
                     GVARYGSFLEPGTILMPGYVNIGAYVGSGSMIDTWATVGSCAQIGKGVHLSGGVGIGG
                     VLEPPTASPVIIEDGAFIGSRCVVVEGVVVEKEAVLGAGVVLTSSTSIVDVTGPEPVI
                     SKGRVPARSVVIPGQLPKQFPAGVYHVPCALIIGRRSASTDQKTSLSDALRTFQVPV"
     gene            complement(14355..14783)
                     /locus_tag="PKL17_10805"
     CDS             complement(14355..14783)
                     /locus_tag="PKL17_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45265.1"
                     /translation="MADSGADEVIIITRVLREAADRMMAQAYTDRDAGKLSLEDFFAV
                     AERYQQIQNECNRAVHEAASRLPKFPGEMVAISQATAQLENALKTISAVSDTVNLSAK
                     LLWALGSLVLTIVTPSLEGAGVTASAIVDVGRTLAEIATR"
     gene            complement(14773..15747)
                     /locus_tag="PKL17_10810"
     CDS             complement(14773..15747)
                     /locus_tag="PKL17_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNK45266.1"
                     /translation="MEWIFAQNIAEALASANEGSRYVGGGLSLTLLREHGVDTPTRLI
                     DLSALPLQRIEGRQGELRLGAQVKIADLARDPMVQTLAPLLRESAAQTATPAIRNQST
                     LGGELLARTRCPLFRDANNLRCSKRIPHSGCALQEGVHASFAVLGGSPACIAPSSSEL
                     AVALGALSAQATLLSQSGTKERVVPLLDLYLAPGETPEREFALEPGELLTSVTIPVQT
                     ARRYGYAKIALSPLHPVASAAVMLEQRGDVIASARVVLGGLTTRPWRSQQSESVLIGQ
                     SPNAALFAKAAAVALADAAKSAGSRAIVDAGKQVIEIALQRVGDSHGR"
     gene            complement(15754..16356)
                     /locus_tag="PKL17_10815"
     CDS             complement(15754..16356)
                     /locus_tag="PKL17_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK45267.1"
                     /translation="MSDERAHDGLSRRTFLSVGAASVVLAPALAHAEKPSEDELPKLR
                     MGEVRLSLTINGTLRNVAIAPEQTLLDVLRDTLGVTSCKRGCETGSCGACTVLFDGKR
                     VPACLIPAPLAHKRSVTTVEGLLQDGKLSPMQQAFLDHDATDCGFCTPGQVMAATAIV
                     EEGWPSSEDALRQSLCGQACACGKKPQILSAIQQVLKQRR"
     gene            complement(16353..18179)
                     /locus_tag="PKL17_10820"
     CDS             complement(16353..18179)
                     /locus_tag="PKL17_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNK45268.1"
                     /translation="MPLRPFLSRTSHRGTSSPLRRSAPRLGSLLRTALPFFLLLTACP
                     RRFDPRAQEVRGSSPNVEAEYRRGEKLLLDGKFAEAERALLEFRRKVGPAEPLYGLAT
                     VMQGRALRQMGQSVQAKELLSPLAASHPESSWLGREVRRELGLIEYGLQNWAASRRWL
                     AALSVDIVDGEEGAELHATLADLHKRAGDVEAALREYERLSQSKAVRPGELLYLRRQS
                     QPLVEQLPQPQRDGWRKRLGLSSPEQVKLEAAQTLRIGLLLPLQGKDRNLGDRVLRGA
                     MWGADLLGGTQQAAPRVDLRIRDSSAGNIAQLVSELAQEGAQVIVTPPTKAEATQVSA
                     EAEKLGMLALCIGPRDAQTTGTFQLLRTNRSRAQALAQHLIKSGLQTVAILAPNTGYG
                     QAMSRAFAETLQGSAVKTVADLSFADGATTFLAEVQKLAKAQPDALFLPVSATQLELI
                     SSQLAAGGVVGTYRVSRGPDGEPQQNGQRIKLILSSAEGMGERLLRSAGRYLQGAVLA
                     PLSVGQLAVTGGQVARLDYGSGEEPTSLDALGFDAVRLLRLACQRLGTADLCTARELS
                     SVLHELSIDGATGPLGFDAAGQRSSSAMLLRVTGQNLEFFPP"
     gene            complement(18191..>19130)
                     /gene="icd"
                     /locus_tag="PKL17_10825"
     CDS             complement(18191..>19130)
                     /gene="icd"
                     /locus_tag="PKL17_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNK45269.1"
                     /translation="NVALRQMLDLYVCLRPVRYFRGVPSPVKRPEAVDMVIFRENTED
                     IYAGIDFEAGKEAAQKTLEFLKQNFPKEFKKIRFGGEDLNSVGVGIKPVSRPGTERLV
                     RSAIEYAIKHKRKSVTLVHKGNIMKFTEGAFRDWGYALAQSEFGGVEIDGGPWTKLPG
                     GIVVKDAIADITLQQVLTRPDDFDVIATLNLNGDYLSDALAAQVGGIGIAPGGNINYV
                     TGHAVFEATHGTAPKYANLDKVNPGSVILSGVMMLEHLGWQEAASLIVSGMETTIGQK
                     VVTYDFARLMDGAKEVKCSEFGTAIIENMRTLNKSA"
BASE COUNT         3759 a         5859 c         5748 g         3764 t
ORIGIN      
        1 ctttcacgaa gtcggggtcg ccgtcccggt tcatgatcat gatgttttcg ggcttgaggt
       61 cgcggtggac aatgccgacg gagtgggctc gctggagcgc cgatgcgacc tgccgggcga
      121 tgtgaatgac ccgcgccggg ctgagcggac cgaagttcaa ctggtcacgc agcgatttgc
      181 cgtcgataaa ttccagaacc agaaacagcg agccttcgtc cgtggttcca aggtcggtcg
      241 cagcgacgac gttgggatgt tccaagtggg ccgccgccat ggcttcgcgc tcgaagcggg
      301 ccaccatttc gcggtttttc gatagctcgg agttgagcac tttgagcgcc attcgcttgc
      361 gcatcagcgc gtgctcgacg agatacaccg cgcccattcc accttcaccg agaagcgaca
      421 cgactttgta gcgtccgccg acggtcgtgc ccaagagccg cttggctggt tcgcccgtct
      481 cgggaccggc tgctgccgga gtttcggcct gatccatcca gactaagata gccgatttgc
      541 gccagtcgcc tgagtaattt ggttttgaaa actacgaaat ctttggtgtg taatgggctt
      601 agcgggcgca gaacgctttg tagtcgacaa gctgaatcgg tcggctctca tcgctgcaca
      661 ccgggcagtc ggggtctcga cgcagcttga cctcgcgcag cgtcgctgcc agcgcatcgt
      721 agagcaaaag tcgtccaacc agtgaatcac ccagcccgag cagcagtttg atggcctcgg
      781 tcgcttgcag tacgcccacc actccgggca gcacaccgag gacacccgct tcctgacacg
      841 acggagccag ctcgggtggc ggcggctcgg gatacaggca gcgatagcac gggccaacaa
      901 atggcaaaaa cgtcgtcagc tgtccctcga aacggtaaat cgatgcgtgg atgaccggaa
      961 tccgcaacat caccgacgca tcgtttaaca gatagcgagt cgggaagttg tccgccccat
     1021 cgacgatcaa gtcatagcca gacagcagct cgacggcgtt gtgttcggtc agccgcacgt
     1081 tgtggctttt gatcttcacg tcgggattca gctgcgtgat cgtctctgcc gcagactcga
     1141 ccttgggacg accgacccga cgggtgttgt gcaggatctg ccgctggagg tttgattcct
     1201 cgacgacgtc gctgtcgatg atgcctagcg tgccgacgcc cgccgccgca agatagtacg
     1261 ccgatggcga tccgagcccg cccgcaccga gcagcagcac ccgacttttt agcagcgtgg
     1321 cttggccttt ttccccgact tcgggcagca ggatgtgccg cgaatagcga ctgcgctgct
     1381 cggcagaaaa cacctgcggt ttttcaattg ggccgcccgc cgtccgccac gctgcgattc
     1441 ccccagcgag cgaacgcaca tggccatagc ccattgcgac gagactgctg gccgcgagcg
     1501 ccgatcgatg accaccctga cagtacaaga cgatgtgtgc gtttggatcg ggcgctacgc
     1561 tttcgatttt tccctcgagc agcccgcgcg gaatcagcac ggcaccagga atcacccctt
     1621 gcaagtgttc gtccggctcg cgaacatcga ccagcagctc atttgcagtg gccaagcgtt
     1681 cagcgcgcag ctcttcgacg gaaagctctt gcacgttggt gcgaatctgg cgcaggtatt
     1741 cttgatagtt cggcatgcgg tttcgcttct tgacgctgcg gcgcgtgggt cataatctaa
     1801 cgttacgtga gcttttgcac tggacgtgtg ggcaattttc accgataaca agcaagcgcc
     1861 atgcgagttt aggcgaccaa ttgttaccaa tgtgcaaaag tggcggcagc aaaatctacg
     1921 ctcaccttag accaagacgc tggccggctc gaatgagact tgccgcattg ccagtaccga
     1981 atctgtggag gaaaccatgt ccgtgattac caccaacatc cgcaagcccg acgccgcgcc
     2041 cgaaccggtg ccggctcctg tggctgcgac ttccccgatc tcgctcaccc aagtggccgc
     2101 cgacaaagtg atggaaatcc gcaaggacga agccatcgag gacaccatgt tcctccgcgt
     2161 ccgcatcgtc ggtggtggct gctcaggttt ttcgtatgac ctgtactttg acaccagcac
     2221 gccgatggac aagcagcttg tctcgaacgg cgtcaccatc atcgtcgatc agatgagcct
     2281 tcagtacctg cgcggcaccg agatcgacta cgtcgaaggt cttcagggcg ccggcttcaa
     2341 gttccacaac cccaacgtca aatcgacctg cggctgcgga tcgagctttt cggtctaaat
     2401 cacgccgcgc cccgccgata ctgcgcgggg cctttccttg gcccgcttac ttgttccccg
     2461 gcagcgacag ctccaccgca aacgaaaatc cctcgccccc ttcgtagctc atcgcgccga
     2521 tgtcgatgtc tgtgtgcgca gtgtcgctga gcttttccgc gagcttttcg acgaacgccg
     2581 tcagcgcatc tggttcgacg ggttcatcaa aacgggtgaa cattcgcacg tagaccggcg
     2641 cttcgatgtc catttccccg tcgcgctggg cccgcgtcag gttgatacgc accggcagct
     2701 cgagcgcttc gccctcgcac cactcgaaca gactttcgct gtcataggtg agcgggccgg
     2761 tgagcccggt ctcctccacc gcttgtgcaa gccatgggag ctgacctggg aagtagagcc
     2821 gaagatcctg cattggctgc tagtgtcgcg cattcgcagc cggcaatcaa aacccaagtt
     2881 ccgacggacg gatacaaaag cgtcgctagc gggccggggg aatcaggtga cgactgaccg
     2941 cggccatgct ggcggcatcg tggacatctt gttccagcag cggaatctgc gacagcggca
     3001 gcttgagttt ttcgccgagc cgtcgaatcg acgcttcgtc cgcagcagcc aacgtccgct
     3061 gttcggcata ggttcgcgaa agctcatcgg ccagcgcggc cagatcgtcg gacgcaaaac
     3121 ccgatagctc ggggcgggtc tgcaaaattg gcagcaggtc cgccggcact tcgcgggcgt
     3181 gactgtggac gcggtttacg atgcagccgc cgatcgacag cttggcgcgg gtcagtcgct
     3241 cgtgaaaata cacggcttcg tcgatgctca gcggctcggg actacacgcg agcaggaagg
     3301 ccacttccgg cttttgcata agctcggtga ctttttgggc gcgctcgcga aaaccggcca
     3361 gcacttgctg aaattcgacg aaaaattccg ccacttgggt caaaaacgcc gaaccaagaa
     3421 accgtcccag ccccttgagc acgatcgtgc cgcccattcc gagcagccgc agcgacagac
     3481 ttcccgcctg ttgcagatac tgtttcatga tccactggat cgtttcggag tcgacggctt
     3541 tggccatctt gtccggcgca tccagaaaat cgattgcatt ttccgtcggt ggcgtgtcca
     3601 tcaccagcaa gtcatagcga tcgagctgcg acagctcata aagccgtgac atcgccgcgt
     3661 attcatgcga acccgcgagc gccgacgaga tttgctgata aatctggttg cgcagcacgc
     3721 ggtcgcgcag cttggggtcc gtcgcataac gcgccaccag atcgtcgaac gcgcgctttt
     3781 gatccagcat catcgcgtcg agcacgccac ctggaaccgg ggtttcgccg cgtgccgccg
     3841 ccaagatgcg cagccgttct tcggggactt tttggatttc gtggctcagc gccgacaccg
     3901 acagcgcatc ggcgagccga cgggctggat cgatggtaag caccagcacc cgtcgtccac
     3961 gcagcgccgc caaaagtccc agcgtcgccg atgttgtggt cttgccgacg ccgcctgaac
     4021 cgaccaccat cacaatccgc ttgcggctga tcacgtcggc gagggttcct gtgctcagcg
     4081 ctgcatccgt cgtctgcgtc atggtgtcga ttggtttgat ggcagttagg ccaaaatctc
     4141 gtcgagtccg cgctcggctt cctcgatcat ctggctgttg ttgagcgtgc cacccaaaat
     4201 tcgcccggtg tttagcaggt acagccgtcc cggtacaagc ggaatgggca gccgcagccg
     4261 atcgtagttt cgcaccgcaa tcggactgac aaaacgcgag cggaaaagat gcgtcgatag
     4321 cacctcatcg agacggaaac cgcgatgccg cgcaatctgt gtcagcgcac gcagaaacgg
     4381 ttcggtaaac gcgctgtcgg gcttgtcgaa atcgggatcg tcgtcgggaa gatagcgtgg
     4441 caaatagacc agatggtggc cgccggtttc gctcggatcg accagcgtgg taagcccaat
     4501 caccccggta aacggcattc ccgcgtcggt gagattcaag atgtaatacg gcagaagcgg
     4561 gcgcaacagc ttgaccacca gacacagcac cccaaggtag cgggtctgct cggcgcggtg
     4621 gagcaggtcc gacggaaccg ggaagcgggc gcagagcttg ggcaaaaagc gcgacgggcc
     4681 ggtgtaaatc acccgatcgg cgaaaagctc ttgtccagac gcggtgcgga tgcaggtgct
     4741 gccgtctgag ttgtcgacaa gctcgctgac cggggcggac ttgtggacgg tgacgccgag
     4801 ccgctgcaaa tactgctcag cggcggaaaa caccggggca tagccgcctg aaatgtaacc
     4861 gaatgcttcg cgtcgacttt cgccttggcg ggtggcgtac agccggcgga tcgtcgacca
     4921 gataaacgtc gccgcgactt cttcatgggc gctgcccagc ttggcgcgca aaagcggctg
     4981 ccaaaacttg cgatacaccg cttcgccggc gatctgggtg agccactgct tcgccgtcac
     5041 attccacagc ggtgttccgt cgctgacgcg cgatgcatac aagatgctcg cagccagccg
     5101 acttttttcg atcaggttga gcggtggaaa gcgcagaaaa tcgatcggcg tgtcaaaagc
     5161 gtgaagctca ccgtcggcgt aaaagccggt atgggcaccg cgccagcgca gcttgccgtc
     5221 gcagccgagc cgtcgcagcc agtcggtaag catcacgtcc tgcggcgtca cgacgtgata
     5281 gaagcgatcc cagccgattg gatcgggctc gctgcggacc gaaagaccgc ccagcgtcga
     5341 cgatccttcc acaagtgtca cgctgctctg cggtcggcgc tgttttaacc gttcggcgag
     5401 tacacagccg gtgacaccgc cgccaatcac cacgtagcgc ggaaaaccgc ttttgcctgt
     5461 gtgcgctgtc atcgtgggcc cgagtataca agccccgtcg ggctgctcgc atagaccttt
     5521 gcttcgctga gtgttagtta cagatgccat gaacattgtg tgtctaggtg gtggtcccgc
     5581 cggtttgtat ttggcgatct tgatgaaaaa ggctgatccc agccaccgca tcaccgtcgt
     5641 cgagcgcaat cgtccggcgg atacgttcgg ctttggcgtg gtcttttccg atcagacgct
     5701 ttcgcatctg cgcgacgaag acgccgaaag ctacgacgcg atcgagcgcg ccttttacca
     5761 ctgggacaac atcgacgtgt tctatcgcga tcaagtgatc cgatcgtgcg gacacggctt
     5821 tgccgggctg ggacggcacg atctgctgcg gattttgcaa aagcgcgccc ttgagctggg
     5881 cgtcgaaatc cagttcgagc gcgagatcca atcgggcgat gtcgattcgt acctcgatgc
     5941 agatctggtg gtcggatcgg atgggctcaa cagcgtcgtg cgcgcccgtt ttgccgacac
     6001 gtttgcgccg cagattgact ggcgacccaa ccgctttgtc tggctcggta gtacgcagcc
     6061 gttacctgct tttacctttt atttcgacga aaacgagcac ggtctgtggc gggttcacgc
     6121 ctatcgctac acccaagatg cggcggaagg cgaaccgcag tcgaccttta tcgtcgagtg
     6181 cacggcggaa acatttctgc gctctggact cgatcccgcc gacgaagacg cgacgcttca
     6241 gtactgtgaa aagctgtttg cgccccggct ggcaggtcac aagctgtgca aaaatcgcag
     6301 tctatggcgc aatttcccga cggtgcgctg cggaaaatgg agccacaaaa acgtggtcct
     6361 gttgggcgat gccgtgcaca ccgcgcactt ttcgattgga tcgggcacca agctggcgat
     6421 ggaagatgtc atcgcgctgt gcaaggcgct gcgcgaaggc gggcgcgaca ttcggcaggc
     6481 actgcaagcc tacgaagccg aacgccgtcc ggtggtcgaa agcttgcagc gaacggcgca
     6541 gacgtcgctg gagtggttcg agcacagcga gcggtttgta gactgcgagc cgtggcagtt
     6601 tggcttcagc ttgctgaccc gatcgctgcg tctgacgcat gaaaacctgc gccagcgcga
     6661 tcccaacttc attgccgaag taaacgatca cgttgcggcg caggccgaac gtcaaaccgg
     6721 ggtcaaagtg ccgcggcatc cgtcgccgcc cccgatgttt acgccgcttc gggttcgtga
     6781 tctgatcatc cccaatcgcg tcgcggtgtc gccgatgtgt cagtactcgg cggaagacgg
     6841 catgccgagc gaatggcact tggttcacta cggcagtcgc gcgattggcg gggccgggct
     6901 tgtcatcagc gaaatgaccg atgtctgtcg cgaaggccgc attacaccgg gctgcgcagg
     6961 tctttacaaa ccggaacatg tgccggcgtg gcgacggatc gtcgactttg ttcacaagca
     7021 cagcgaagcc aaaatgggcg ttcagcttgc gcatgcggga cgcaaagcgg cgacgtgttt
     7081 gccgtgggtg cgggaagatg aaccgctgcc cgaacaggaa agctggccga tcatgggtcc
     7141 gtcggcgatt gcctggaatg aacgcagcca gattccgcgt gaaatgacgc aaaaagacat
     7201 ggaatcggtg ctggctgact ttgtcgcagc aacggaaatg gccaatgaag cgggcttcga
     7261 tctgctcgag ctgcacatgg cgcacggata tctgctgtcg agcttcatct cgccgctatc
     7321 gaatctgcgg caggatgaat acggcggcag tttgaaaaat cggatgcgct ttccgctgga
     7381 agtgtttgcg gcggtgcgtg cggcgtggcc cgacgaaaaa ccgatttcgg tgcgtgtgtc
     7441 ggcgcatgac tgggcaccgg gcggactgct tcccggtgat gcggtcgaag tggcgcggat
     7501 gctcaaagcc gcgggggccg atatcatcga cgtgtcgtcg ggcggtacgg cacccgaagg
     7561 tcgtccgcag tacggacggc tgtatcagac gccgtttgcc gaagagatcc gtcttggcgt
     7621 gggcatcgcg acgatggcgg tcggcaacat ttcgtcgtat tccgacgtaa acagcgtcat
     7681 cgcagcggga cgtgcggacc tggcgctgct ggctcgggcg cacctgtttg atccgtactg
     7741 gacgcgccat gcggcggcgg atcagggcta ccagatgaag tggccggatc cgtacctcac
     7801 cgtcgcgaac taccgtcctc gctttaagta attcgatccg accttgcggt cgccaaacaa
     7861 ccagtcagga gaaatgcacc atgaagaacg cagttctgtt tcgctcgacg tttgcgctgt
     7921 gtctgccgct tgccacgttg gcaatgtcgc cccgcttggc gagcgcgcag cagcttgagc
     7981 tgccccggcc cagcccgctt gcgaaagtca gccagatggt ggggcttacc gaagttgcga
     8041 tcgaatacag cagcccgcag gccaaaggtc gcaaggtcta cggtggcgtg gttccgctcg
     8101 acaagctgtg gcgaaccgga gcaaacgcgg cgaccaagct gacgtttagc aaagatgtca
     8161 ccgtcgacgg caagccggtt gctgcgggtt cttatgcgct gtttacgatt ccgggcaaag
     8221 acacctggac ggtgattctg aacaagaatc cgaaccaagg cggcaccgga cagtacaaac
     8281 aggatctcga tctgttgcgg cttaccgtca agccggaagc ggttccccat cgtgagcggc
     8341 tgacgtttct gtttgccgat acaacggaaa attcgacgcg cattgatctg gaatgggaaa
     8401 agctgcgggt gtcgattccg gtgaaagcag cgacggaaac gcaggtcgat gcgggaatca
     8461 aagcgctgga agaaaactcg tggcgtccgt acaacaatgc ggcgcgctac cttctggaaa
     8521 gcaaaaaaga tccggagcgt gcgctccagc ttgtcgaccg ttcgctgtcg ctgcacgaag
     8581 actggctgaa caactggacg aaagcgcagc ttgtgggtgg aaaaggcaag acgcaggaag
     8641 cgtgtcagct tgcgcagaag gcgaagacga tgggcgaaaa gaatcccgat ggtttcttct
     8701 ttgccgacga agtgaaaaaa gcaatcgccg actggaagtg ccgctaactg ggtctttaca
     8761 ccacttcgtt tggggggcga agtggccgcg gtctttcccg cggcgtggaa gggcttgcgt
     8821 ctgcgcttgg gagtcagtaa gaagcaggtt tcggactgag tccgcttcga ggctcagtga
     8881 acgattacct ggtaattacc gcactcccaa ggaacaacga tggacaccgg atcgacgcct
     8941 ccgaccgccg attttgagtc tgctttccca tcgctcatcg actgtgaacc gagcgattcg
     9001 gatgtggatc gctggcagcg ggaagcgcgg gaacttttgc gccttgcgat ggagcgcacg
     9061 agccgaatcg tcaagcagct gggtccgtcg cgacaaggac tgcgcacaca cctggccaac
     9121 ttgcagctcg cgtatgacta tttggccgac ctaaatgaag agtcggacga tggccggacg
     9181 ccgagcaagt cgctgaacct ggggctttcg cagagacagg acatcgccaa gatggtgcgc
     9241 gaccggcgca tgagcctggg tctgtcggtg ctggcgctgg cgcagcgcgt cggtttgtcc
     9301 gaagggacga tcaaaaacct ggaatcgccg cgcaaaccga tcacccggac gacgcttttg
     9361 cgtctggccg ccgcggcgga gcttgggctt cagtttgacg acatcctgtt tgatcagccg
     9421 agtcccgatc agggtgagcc gatgaactgt tttatcgcgc ccggcttcaa tccggtgcag
     9481 atggtcatcg agctgggaga aaagctaaat tccgtcggtg gcttgatcga acagaccaat
     9541 gtgtacctgg atcaccaaag tgcagcggcg tatttgaagc tggccaacca gctcgactac
     9601 gtggcggcgt tccgcgatcc ggtgccgatg caaaaaatcg ctgacgtgat cttggaatcg
     9661 accgggcgcg ctggcatcga cgtgatcgcg ctcggttcgg gggatgcgcg cgaagaagtg
     9721 cggctggttc agcacctgat tgcggcgtca catccgccgg atctccgtct ttacctgtta
     9781 gatgtcagtc aaccgctgct attaaccggt tttatccatg cggcagagac gttggctgac
     9841 caatacggcg tggcttattt tgcaatgcaa ggcaattttc atcatttacc tagatatacc
     9901 cagctcaact atacgccaac gcgatcgcat cgtcgccgag ttgccacaat tttcggcggc
     9961 acgatggcca atttggagca agaaggtcag tttttccgtc acagcttgtc gggtctggct
    10021 ccgggcgata ttgcggtggt ttatgcacaa attgcagttg cgcctcccaa ccaaccggac
    10081 gtaattttgc gattagagcc tgcgctgcaa aagccgatgt cggctgcgca caagaactgg
    10141 cttagcgatc ccatctatag gcattgcaag gaggtgaccg atgttgagct gcatttagag
    10201 ctaaacacgt cttgtccggt gcctggaagc tatgcactcg aagcgattgc aacggtgaag
    10261 cttacagggc gacggcagaa ggtcttttcg atgcatcgct ggcgcaagta cgatcctcag
    10321 cttttgtcgg aacacctcaa gacgatgggc tgggatacgc tcgctttgct gcccagcggc
    10381 agctccccca aaaaccaagc gtcagtgctc gtgctgcgca aacgtaacac tgcgtgacgc
    10441 actgcgcccg gcatggcgtg aagcgcgacc tctgcgatgg tatgatgatg ccatgcgcgc
    10501 catcgcctcg acgacagaat cgctgaagct gtggggtatt ggccgcgtac atcggaagga
    10561 ggcggtcatg cgaaaaaaca acctgacagc caaactggcg atgggccttt acgcgatggt
    10621 gctgggaacg gcggaacctt ccgcaaaggc cgcgtcgcca cgactgccgc aggacgcgct
    10681 cccgatcccg ctggttcgac agcagtcgga tcatagctgt gggccggcgg tgctggttgc
    10741 ggtgctgcga tactttcagg tctgggacgg gccggaaacc aacctgcatc ggctgctcgg
    10801 aacgtcggcg gaccaaggca cgctgcccga agcgatcgcg tttggcgcgc ggcggttcgg
    10861 tctgcaagca tccgtcgagc agcccatgac gctggtgcag ctacaatcgg cggttcgcgc
    10921 gggcaagcca gtgattttgg aactgcaagc gtggcgcgat ccgaaaggcc gtcgcgagtc
    10981 ctggctcaac aactgggatg acggccacta tgttgtgctg accgggatgg accatcagta
    11041 cgcatacttt atggacccct cgacggacaa cgcctatacg tatgtgccgc tacaggaact
    11101 tctgtcgcgc tggcacgacg tcaatccagt ccgaggtccg cgcagccagc acgactatca
    11161 cttgggaatt gtgatctcgg cgccggcttc gatcgcgcgc catcgcggca tcacccgtcg
    11221 cagcaagatc atcgatcgcc tgataaagct cgactgaaaa cggcctgcgg ttagctgagc
    11281 gcgcgtttga tgcgacggat gccttcgcgc agaagttccg gacgggccac aaaggtcacg
    11341 cgcaaaaatc cttcggcgcc aaaaatgctc gacggaatcg tgaccacgtt ctgttcttcc
    11401 atgatcttga ccgcggcatc catcgtgcga tgtgcccacg catcgggaag tcgaatcagg
    11461 cagtagaacg aaccttccgg tgtcactgcc gaaagacctt cttccgccag cgcatcgagg
    11521 agcacctgtc gttgcgaaag gtagtgcggc ctgtgtttcg acagcagctc gggctgacgg
    11581 aaaatttcaa tcgcggcgcg ctgcgcaaag gtatcggcac acgacaccac aaactgatgc
    11641 gctttccaca tcacctgcgc ggcggcctgg ggcgccatga tccagccgag ccgcagtccg
    11701 gtcagggcac agctctttga cagcgacgat gccacgatgg tgtggggata aaacgcagcc
    11761 ggcgagggcg gtctctgctc ggtaaaatac agctcggcat acacttcgtc cgacagcaga
    11821 tacggcggct gcggcagtgc aagaagtccg tcgctgagct tgcgcagctc ggattccggc
    11881 cacacccttc cggtcggatt gcacggtgat gcaatcgcaa ccagccgcgt gcgatccgta
    11941 atctgcgaca gaatggccgc agcgtcgggc gcaaagtctt ttttcggatc gagctgcact
    12001 tcgacaacgc ggattccttc gatgtggcac agcttctggt aggcagggaa cgtcggtgtc
    12061 accaccagcg cttcatgcaa cgacggatcg agcagtgctt tgatcggcag atacagcgct
    12121 tcctgcgaac ctatcgtgat gcagacttgc tcgggcgagc gcagccccgg ataggcgaag
    12181 tgctgggcaa tcgcggcgcg cagctcgacg aagccggcgt tttgcgaata cggacagccg
    12241 tgctcgcgaa tccacttgag cgcagcttct aggggcgcca tgtcgggatg gagctgcggc
    12301 tgacccattc ccaagtcgat ggcatcggct cgcttgcggt cgtttagcat ccggatcgca
    12361 gaaggaggaa tcgtttcgac aagacggttc atcgcgtgct ctctcctacg ttcgtcacat
    12421 cagccacgct gccgcagtag ctgacccagg agcttgtagt tttccagcgg cccggccaaa
    12481 agacaccact cgccgacttt gtgcgcttgt tccggatcgc cagggccaca gtttaccgcg
    12541 gcaatcccca gtccggtgag ccgcgccaca tcggtccatg cttgcttggc agtgacggcg
    12601 gcgccgcttt gctttatcca cgaagacaca atcggatggt cggtacagac agcccccgac
    12661 ggggaagcgt cgaccaagtg gacgtcgaag cccggaccga caaattctcg cagctcggct
    12721 tcggcggtgt ccagagtctt gcccggacag aatcggtaat tgacgtttac catcacccga
    12781 tccggaatca cgttgcgtgc gttttccgtc aggatttgcg tcgctgacat cacttcataa
    12841 aacgtgagcc caagcttgtc gacagcgcgt ctttgccaac cggccagctt ttggagtagt
    12901 ggcgtcacct gataaatcgc gttttcccca agccacggac gggcggagtg cgcgcgtttt
    12961 cctttggcga acacctgcgc ttgcagactg ccggaacagc ccgcctcgat gcgattgctg
    13021 gtcggttcca gacacaccgc ggcggtcagc gtccgcagga aatcgccttc ggcttcgcac
    13081 agcggcacca tcccgcttag gtgaaccggt ccttcctcgc ggtcataaaa cacacagaca
    13141 agctcgcagg gcagcgattc agcatcggca atcagccgca gcatcaccgc cagaccggac
    13201 ttcatgtcgc ttgcgcccag accgtaaatc cgaccgcttt gccagtcgat gtggtgcggc
    13261 tgcgcgctgt tgggcggcac ggtgtcggaa tgaccaaaca gaccgattcg tgggccattg
    13321 ggcttgccac agcgcacgag cagcgcattg ccaatccgct tttgctcggc gtgcggctgc
    13381 aagctgcgga cgcgatcgga cagatggctg gtaatctgcg cttcatcgcc cgtcaccgac
    13441 ggaatcgcac acagtgccag cgtttcttcc gccagccagc ggcagagcgc atcatcggaa
    13501 agcggggtct gcatcatcgc ttacaccggc acttggaacg tccgcagcgc gtcggacagc
    13561 gaagtttttt gatcggtcga tgcactgcgc cgtccgatga tgagcgcaca cggcacatgg
    13621 taaacgcccg ccggaaactg cttgggaagc tgaccgggga tgaccaccga acgcgccggc
    13681 actcggcctt tgcttatcac gggttcgggg ccggtcacgt cgacgatgct ggtgctggat
    13741 gtcagcacga cgcccgcgcc gagcacggct tctttttcga ccaccacgcc ttcgacgacg
    13801 acgcagcgtg aaccaatgaa cgcgccatct tcgatgatga caggacttgc cgtcggaggt
    13861 tcgagtacgc cgccgatgcc cacgccgccc gacagatgga cgcctttgcc aatctgcgcg
    13921 cacgaaccga ccgtcgccca ggtgtctatc atgctgccgc tgccgacgta agcgccgatg
    13981 tttacatagc caggcatcaa gatcgtgccg ggttccaaaa acgaaccgta gcgcgcgacg
    14041 cccggcggca cgacgcgaat ccctgcttct tttaggtttt gtttcgtcgg cactttgtca
    14101 aaaaacgaca gcgccccggc gtccatcggc tcaagtggtc gcagcacgaa atacagcagc
    14161 accgcttgtt tgacccaggc attgacgcgc cagccatttt cacccggttc agcgacgcga
    14221 atgcggcctt tgtcgagatc ctcgatggtt gcaaaaatcg cctgctgcac gtcgggatct
    14281 ttgagcaagc cgcggtcaga aaatgcggcg ctgatggtgt tttgacggtt cgatgttgtg
    14341 ctgtgcatgg tgtgctaacg ggttgcaatc tcggccagcg tccggcccac gtcgacgatc
    14401 gccgacgcgg tgacgccggc tccctcaagg ctcggcgtga cgatggttaa taccagggaa
    14461 ccaagcgccc acaaaagctt ggccgacaga ttcaccgtgt cggagacggc agaaatcgtc
    14521 ttcagtgcgt tttcgagctg cgcggtggct tggcttatcg caaccatctc gcccggaaat
    14581 ttgggcagac ggctggcggc ttcgtgcacg gcgcggttgc actcgttttg gatttgctga
    14641 tagcgctctg cgacggcaaa aaaatcttcc agcgaaagtt tgccggcatc acgatccgta
    14701 tacgcctgcg ccatcatgcg gtctgccgcc tcgcggagca cgcgcgtgat gatgatgact
    14761 tcgtctgcgc cactatcggc catgcgaatc ccccacacgt tggagcgcga tttcgatcac
    14821 ctgcttgcca gcatcgacaa tcgcccgact gccagcgctt tttgccgcgt ccgcgagtgc
    14881 caccgcagcg gctttggcaa acagcgccgc atttggactc tgtccaatca agaccgactc
    14941 gctctgctgt gatcgccagg gccgtgtcgt aagacccccc agcaccaccc gcgcagaagc
    15001 gatcacgtcg ccgcgctgct ctagcatcac cgccgccgaa gcaaccggat gaagcggcga
    15061 aagggcgatt tttgcgtacc cataccggcg cgccgtttgg accggaatgg tcacacttgt
    15121 cagcagctcg ccgggttcca gcgcaaactc gcgctccggc gtttcgcccg gcgccagata
    15181 caggtcgagc agcggcacga cgcgctcttt tgtgcccgat tgggacagca gcgtcgcctg
    15241 cgccgacagc gccccaagcg caaccgccag ctccgacgac gacggtgcga tgcacgctgg
    15301 actgccaccg agcaccgcaa acgacgcatg tacgccttct tgcagcgcgc agcctgaatg
    15361 cgggattcgt ttgctacagc gcaggttgtt ggcgtcgcgg aacagcgggc agcgggttcg
    15421 cgccaagagc tcgcccccaa gcgtcgattg gttgcgaatc gccggggtcg cggtttgggc
    15481 tgccgactca cgcagaagcg gagccagcgt ctgcaccatc ggatcgcgcg ccaggtcggc
    15541 aattttcacc tgcgcgccca gtcgcagctc gccttggcgt ccctcgatgc gctgcaaggg
    15601 cagggcggat agatcgataa gtcgcgtcgg ggtgtcgaca ccgtgttcgc gcagcagcgt
    15661 cagcgacaga cccccaccga cgtaacgcga gccttcattg gcactcgcga gcgcttcggc
    15721 gatgttttgg gcgaaaatcc actccatcct gaatcaccgt cgctgcttta gcacttgctg
    15781 aatcgccgag aggatctgcg gttttttacc gcacgcacaa gcctggccac acagcgactg
    15841 gcgcagcgcg tcttcgctcg atggccagcc ttcttcgaca attgccgtcg ctgccatcac
    15901 ctgaccgggc gtacaaaagc cgcagtcggt cgcatcgtga tccaaaaacg cctgctgcat
    15961 cgggctcagc tttccgtctt ggagcagtcc ttccaccgtc gtcacgctgc gtttgtgggc
    16021 gagcggcgcc ggaatcagac acgcaggaac acgctttccg tcgaaaagca ccgtacacgc
    16081 gccacacgat ccggtttcac agccacgctt acagcttgtg acgcccaggg tgtcgcgcag
    16141 cacgtcgaga agcgtttgtt ctggcgcaat cgccacattc cgcagcgtgc cattgatcgt
    16201 cagcgacagc cgcacttcac ccatgcgcag cttcggcagc tcgtcttcgc ttggtttttc
    16261 cgcatgcgcc agcgccgggg caagcacaac cgatgccgcg cccaccgata ggaaggttcg
    16321 acgcgacagg ccatcatgtg cccgctcgtc gctcatggcg ggaaaaactc caggttttgt
    16381 cccgtcaccc gcaacagcat cgccgacgac gatcgctgac cggctgcatc gaaaccgagc
    16441 ggtcccgtcg cgccatcaat cgacagctcg tgcagcacgc tcgacagctc gcgggcggtg
    16501 cacaaatccg cagtgcccag ccgctgacac gcaagtcgca gaagccgcac cgcatcaaag
    16561 cccagcgcat caagtgacgt cggttcctcg ccgctgccat agtcgagccg cgccacctga
    16621 ccgcccgtga ccgccagttg gcccaccgat aggggcgcca gcaccgcccc ttgcaaatag
    16681 cgccccgccg accgcaacag ccgttcgccc atgccttccg ccgacgacag aatcagcttg
    16741 atgcgctggc cgttttgctg cggttcaccg tcggggccgc gactgactcg ataggttccg
    16801 accacaccgc ctgccgcaag ctgcgacgaa atcagctcca gctgcgtcgc agaaaccggc
    16861 aaaaacaggg catcgggctg cgctttggcc agtttttgca cttcggccaa aaacgttgtt
    16921 gcaccgtcgg caaacgacag atcggccacc gttttgaccg ccgatccttg cagggtttca
    16981 gcaaaggcgc ggctcatcgc ctgtccgtag ccggtattcg gcgccaggat cgccaccgtc
    17041 tgaagaccgc ttttgatgag atgttgcgcc agtgcttggg ctcgactgcg attggtccgc
    17101 aaaagctgaa acgtgccggt tgtctgggcg tcgcgcgggc caatgcagag cgccaacatc
    17161 ccgagttttt cggcttccgc gctgacttgc gtcgcttccg ctttcgtcgg tggtgtcacg
    17221 atcacttgcg cgccttcttg cgcaagttcg ctgaccaact gcgcgatgtt tcctgcgctg
    17281 ctgtcgcgaa ttcgcagatc gacgcgcggt gccgcttgct gcgttccccc gaggagatcc
    17341 gccccccaca tggccccgcg cagcacccga tcgcccaggt tccgatcctt gccttgcagc
    17401 ggcaacaaaa gtccgatgcg gagcgtctgc gccgcttcga gcttgacctg ttccggactg
    17461 ctcagaccca gccgcttgcg ccatccgtcg cgctgcggtt ggggcaactg ctcgacgagt
    17521 ggctgagatt gccgacgcaa atacaaaagt tcaccgggcc gcaccgcttt cgactgagac
    17581 agccgctcgt attcacgcag ggccgcttcg acgtcgcctg cccgtttgtg caggtcggcc
    17641 agcgtcgcgt gcagctcggc accttcttct ccgtcgacga tatccaccga aagtgccgcc
    17701 agccatcgac gcgaagccgc ccagttttgc agcccatatt caatcaagcc cagctcgcgg
    17761 cggacctcgc gaccgagcca gcttgactct ggatgacttg ccgcgagcgg cgacagcagc
    17821 tcttttgcct gcacgctctg gcccatttgc cgcagcgccc gtccttgcat caccgtcgcc
    17881 aaaccataaa gcggctcggc tggaccgacc ttgcggcgaa attctaaaag tgcccgctcc
    17941 gcttccgcga acttgccatc gaggagcagc ttttcccctc gccgatactc tgcttcgacg
    18001 ttcggcgacg agccgcgcac ttcttgggcg cgtggatcaa atcgccgcgg gcacgccgtc
    18061 agcagcagaa aaaacggcag cgccgtccgc agcagactgc cgagtcgcgg ggctgaccgg
    18121 cgcagtgggg aagacgttcc acgatgggaa gttcgggaaa gaaaagggcg gaggggcatc
    18181 gcgggggagt ttaggccgac ttgttcagcg tgcgcatgtt ctcgatgatg gcggtgccaa
    18241 actccgagca cttcacttct ttggcgccgt ccatcaggcg cgcaaagtca tacgtcacga
    18301 ctttctggcc gatcgtcgtt tccatgccgc tgacgatgag gcttgccgct tcttgccagc
    18361 cgaggtgttc cagcatcatc acgcccgaca ggatcaccga tcccggattc actttgtcga
    18421 ggtttgcgta cttgggcgcc gtgccgtgcg tcgcttcaaa caccgcatga cccgtgacgt
    18481 agttgatgtt gccgcccggt gcgatgccga tgccgccgac ctgggccgcc agcgcgtccg
    18541 acaggtagtc gccgtttagg ttcagcgtcg caatgacgtc gaagtcatcg ggccgcgtca
    18601 gcacttgctg aagcgtgatg tccgcgattg catccttgac gacaatgccg cccggcagct
    18661 tggtccacgg gccgccgtcg atctcgacgc caccgaactc gctctgggcc agggcataac
    18721 cccagtcgcg gaacgcgcct tcggtgaact tcatgatgtt gcccttgtga acgagcgtca
    18781 ccgacttgcg cttgtgcttt atcgcgtatt cgatggcgct gcggaccagg cgttcggtgc
    18841 ccggacgcga caccggcttg atgccgacgc cgaccgagtt cagatcctcg ccgccgaagc
    18901 gaattttctt aaactctttc gggaagtttt gcttgaggaa ctccagcgtc ttctgcgcag
    18961 cttctttgcc cgcttcaaag tcgatgccgg cgtagatgtc ctcggtgttt tcgcggaaga
    19021 tcaccatgtc gaccgcttcg ggacgcttga ccggcgacgg cacgccacgg aaataacgca
    19081 ccgggcgcag gcagacgtac aggtccagca tctgccgcag cgcgacgttg
//
LOCUS       DAORGN010000082        19104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1142_length_19104_cov_5.912226, whole genome shotgun sequence.
ACCESSION   DAORGN010000082 DAORGN010000000
VERSION     DAORGN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_19104_cov_5.912226"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKL17_10830"
     CDS             <1..940
                     /locus_tag="PKL17_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45270.1"
                     /translation="MTATPPRLLQRYNDPMIPPALDDLVARLLLKEPAKRPSAAETSR
                     ELEQILSNLYDSARSPQTLHDQATQELPLLHRATQVVGAVERKTMVVERPDRPTQVLG
                     RDNWPTQLLPQVVTEAKAEAQAVADAEAYARLAKELFAQAVELADRICTGSWPSALKN
                     LGSKVADCYKQEVELAEKLFSLEKLLADKAAASGQHNALRQQILALSEQLQADAAVNE
                     DDRKRLTAELEQKERAFFDAEARWRRLPPTSPDSLRRRIADVRRERVRAQILFVRLIV
                     EMPTAGALLAQRDQLAARLFDIDNPKRSGPQGAVTK"
     gene            955..2364
                     /locus_tag="PKL17_10835"
     CDS             955..2364
                     /locus_tag="PKL17_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK45271.1"
                     /translation="MSSLYLLPLALSLLVDPQLATVAAVPAAQVVEKDLAPSSRFAIS
                     PYVQDVQKDSAVIAFATDAPSMALVHFDGPGGRGAVMSGPQQHHKVRLTGLSAGTRYR
                     YTVAVRRQGDLTGVESMSESAEFATMAEGLPFLFLVYGDCRDRDSDHQAVVRAMLKER
                     PDFVLQTGDMVSRAGDVGQWRRYFAAVSPLIRSVPMYPALGNHELRGDPDASHFFRYF
                     VIPGDRKRRPVYYAFRYGNSLFVSLDGNSPLDHDQSVWLERTLAEASQDRSISHMFAF
                     VHQPPYSVGAYCGSDRIQRRLVPILRRYGVRAVFAGHEHAYQHLERGGLRYFVTGGGG
                     APLYTRSTSCNFEDDMALRLFRAEHHYLRIQVDGDAATLMAVSKDGEVMERVLLHEPV
                     VPDSDYRVGPPVLSQSRVPPSPPVGASSPGQPSRTRPSAPLFLLLTSALAMVVGLLLL
                     TAPRSRRRVDLMPDARRRS"
     gene            2863..4173
                     /locus_tag="PKL17_10840"
     CDS             2863..4173
                     /locus_tag="PKL17_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45272.1"
                     /translation="MSRKKTVAFCVLGGTALSVIAMSCTDYLTGQTPEPDGPLLVTKL
                     TLLDKVSRDKAVFTDTSLPNCMQYPADVCSQDANRNSFACRTCYNDVLKDTYSLAKSP
                     PTPDSATDLRVVFNKIPRKWNGSALNPDDVPDMALRLQCGGTCQGIPTYKRLLDISGS
                     SLSYDPTDIPYGPSLRIAVDRSVDPRVALEPGASYSVFVDVGLSDRSDNRLDGAQAQA
                     LLSFKTEPLTVLQMGRGDSKADPYVYASTKYGDGGTGNPYSIADLPRNSAIVLKLNAS
                     IHPDALGPLKLTGSFKRADGSTGAVDVLVGTNIWATGTDGSVTRDPKTMGCIQQNQRL
                     LAMWPDTPDLKWPMGAVEVTFTLTGGTVKDVAQKAGFPMGVHALAGDVTVRARLTTAD
                     APSGYTGLTTQRAKVSAGACPQLGGADMATLPDMAVSDMTAKDM"
     gene            4192..7569
                     /locus_tag="PKL17_10845"
     CDS             4192..7569
                     /locus_tag="PKL17_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45273.1"
                     /translation="MKRTAKNRRFRLRLPLTLALGGVCAAPFVPGVAHAQLQLGQIQG
                     VITNQDNGKPLQGVTIVVSGPALQGEQSEITDKSGSYLITQLPPGDGYIIRFYFGDVV
                     VERPGVRISQSKTLTVSLSMPTQKGKNVYVVKERAPAVDTATANTGVEINQEILQNTA
                     VRGRTFESAMSLAPGSADVAPRSQAGGDVGVSFAGGTGNENAVLVDGLNTTDLGTGVV
                     ATQLHQYFIKDLNIITGGYQAEYGRATGGVVSIVTKTGSNEFHGGVFGSWSPYQATPT
                     TVARLGEALAFRSKQFQQYDFGFELGGPILKDRIWFYVGFAPTFTINRTERVVRTQVK
                     SQGKRPDGSWKAEIDPDYRDPDYLASDILYAPLRSLALRTEEVPEESRLLDEYARLYN
                     WIAKLQFNLSPDHNITLGYIGAPEFRSEYDGYASDTDATKIDRSKQIHDATVHYVGKL
                     FDRKLQIDVIYGYHYQGLQELPNAENKQYFRYRSPANDDLRGDLAFSLGDFENISRCR
                     VELDPTTRKTLWNPCPVTSYVKNGYGQYKPNQVLQRHAVQASGTFYGKLLGTHGIKLG
                     FDFEDNLSDNTRKYSGVDFDPSDPASGSFNGRLAYETDASGTGVRIRRGFGTFEPFNR
                     FGDAGVPCGVNKDRYCYNSFRAVTETRNYALYLRDQWSVANVGLVLNAGIRWEAQEIF
                     GADGSKQIAIYDNVAPRVGATWDPTRKGRAKIYAQYGRFYQSIPMDINDRSFSGEGLL
                     VGSSFTKDCAKESISPTGIPVVVAKGAPGSPCSLSDPRVSGGGVFAPVAPYLKGQFLD
                     EVVGGAQYDLGLDIVVGAYYTYRWLGSIVEDLSVNGGNTFFIANPGAEPDPKVAADLQ
                     TEADRLKRDASAAPTDAKLQKDADMAQRRAEVYKAQALFPKATRDYHAITLTLQKRLS
                     NRFSILANYTYSRLLGNYPGPFSPYNNQLDPNISVQYDIIDLTVNRNGPLNNDRPHNF
                     KATGFFQQPVFGGSGTIVASLTFTAISGRPIQVLGQHNAYGARNTFILPSGSAGRTPT
                     ITQLDAHLAYEHQLTKEVKLSVYGDVVNLANQRAVANVDDEYTFSVVNPIAGGKVEDL
                     PALRTLDGQKPILNSNYGQPTAYQSPLYLRFGARLSF"
     gene            7570..10128
                     /locus_tag="PKL17_10850"
     CDS             7570..10128
                     /locus_tag="PKL17_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNK45274.1"
                     /translation="MKKLIGSVIASCAVAVVSLGAVARAQVVAFDPASLTPYFASGPL
                     LKASERLRAGDATGARALFTDYLTAWRQEQTASKKDARDDAMDVRVRYLLGLSLLRTA
                     EQLPPGPERVATATGAAQHFLALSKSYPLLASYHALYGAQSLLLAGRPKDSLTQLAAV
                     PTDSVLDCEARFLRGDALLRAAELEPKSQAALREQAVLAFRAYLSACSDDGARKLRAQ
                     VQTAQILDTLSRTAEALQIWRRLYIEAPTESAGVLAGKRLEQPGVKQPPFSASELLDR
                     AQVLFDEMRNPESEAAFRLVLEQPELDDRQKCIARYHLAQSVFKQRQRPRAAPLFDDA
                     VAACAPAGAKNDDLHMKSLYQGARCHASAGRLQKGADLFAEAEAAHPGHSYADDSRLR
                     QAEMYLDLAEKLGRDGAKKTCDAEQCPDYEAKFESLLSDLPTRYPDGDRRAEALWRLA
                     LRALRKKDPSKAKTWLLGSLEKIPREVGWDQEGRTLYWLGRLAEQSDEKTAASDYYQK
                     TARTYPLSFYALLALNRLRDGFATEFDALLRELYGEPDEVEKAPPLTFLPRALYGMPG
                     FLRGLELLRLGLGASARREFAAVGIVVPKSKDALAGKEGDDAQTERLWLASVLYERAG
                     DWHRSHFIPRHILTEWQKHYPKGPWRAYWLLGYPRGYAELLTAAAQQNGHPEALQFAI
                     VREESAFDPLTESFANAIGLTQMIPPTAKRFSGGLPFDREALRDPATNVAIGSRFLGF
                     LWQTMNHHPSLTISGYNAGEGSVFRWLRNYADLKEIDLFVEAIPYDETRGYTKRVLSS
                     YLTYSWLAPLPAGTPLSQRVPVLPFLLPPPGSKGKLSDANPAQAAPANREKPNP"
     gene            10604..12871
                     /locus_tag="PKL17_10855"
     CDS             10604..12871
                     /locus_tag="PKL17_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNK45275.1"
                     /translation="MKIAFTYNLRLSDAEEEAEFDSPETVEAIASALTVAGHEVQKIE
                     VSGPASHLAARLEAFSPDLIFNVAEGKRGRAREAFYPALFEELGYPYTGSDAHTLTVT
                     LDKWLTKRVLASHGMDTPRGRLFTHRDLAEVNKLDGFLGGSLGVPIPAIVKPNFEGSS
                     KGISDESVTRDWKSLRTVVTKMLEQYPLGVLVEEFIAGIDVTVPYIEGVGDPAGEGIL
                     QPVEFSVDPQVRSKYNLLDYRLKNQKSDKVRLRCPADLPRDVIARLRVLAKQVVKLLS
                     IRDVARIDFRLAEDGRIYFLEVNALPALDPAASFFVAARREGLSFGDTINAVLSSAAV
                     RWKLLPSVSAKPKASLKGEKLRVGFTYNVKRPGGKALGDAEAEYDSPATIAAITSALE
                     SLGHEVVQLEANGELPTRLKDAQVDLVFNIAEGVSGRNREAQVPALCELCGIPYTGSD
                     AATLSLALDKALTKRLLKQRNINTPEFQVFHTGREKISPTLRFPLIVKPIAEGSSKGI
                     AGKKSVVDDVATLRKVVLELIQKYRQPALVEEYVTGREFTVGLLGERRPRILPPMEIV
                     FNDHSDKRPVYDYTYKQDWDSRVSYQCPAKLSPKELKAVEKAALDTFLALDCRDVGRV
                     DLRMDAHGNVYVLELNPLPGLTPDWSDLVLIGKAINMDYRTLIAEIMAGGLKRLREKK
                     RGALPPRNGKSNESKSSVSSPSTGVRGEDGDGAAPAAEHTLPAAHAVAGEKAAASPKS
                     RAKGSSRRRPAAASH"
     gene            12883..14790
                     /gene="glgB"
                     /locus_tag="PKL17_10860"
     CDS             12883..14790
                     /gene="glgB"
                     /locus_tag="PKL17_10860"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNK45276.1"
                     /translation="MSLLSDTDLYLFNEGRHLRLYDRLGAHLGTALADGTEVSGCHFA
                     VWAPNAAKVSVIGDWNDWNPERNPLTVVGASGIHYGFAKDVKEGQRYVYQIVSQHGAY
                     TVDKADPVGFSAEVSPAKASVVTRLDYDWGDADWLQQRRKKNALSAPQSIYELHLGSW
                     QRGEGGRFLSYREVAPRLCEYVKRMGFTHVELLPIMEHPFYGSWGYQCTGFFAPTSRY
                     GSPQDFMFLVDSLHQAGIGVILDWVPSHFPADSHGLGFFDGTHLYEHADPRQGFHPEW
                     HSLLFNYGRNEVRSFLLSSALFWLDRYHIDGLRVDAVASMLYLDYSRKEGEWIPNRQG
                     GKENLDAISFLRQLSEDVYREFPDCETIAEESTSWPLVSRPTSLGGLGFGLKWDMGWM
                     HDTLKYFALDPVHRKYHHNLLTFRGMYAQSENFVLSLSHDEVVHLKGSLLTKMGGASA
                     DDWQKRASLRLLFGYQYALPGKKLLFMGAELGQWREWNHEGSLDWFLLDTPEHLGIQR
                     FLADLNRVYSAEPGLSARDFSEDGFVWLEADDQDHNVLSFLRQGETPEETVLVVCNFA
                     KVPWEGYLLGVPCAGYWQELLNSDASVYGGSGGGNLGGCQSESVPAQGQPHAIRAFVP
                     PLAVLYFRRVK"
     gene            14846..15742
                     /locus_tag="PKL17_10865"
     CDS             14846..15742
                     /locus_tag="PKL17_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNK45277.1"
                     /translation="MSIVINTPSGNIGRALANLLLDAGEQVTVISRHPDKVADLTQRG
                     ARLVVGSIDDKDTLREAFAGQKQVFWVTPPPARPDFADWNGATARLAAEAARAAGVQR
                     AVILSSFLADSGPGAGPISTLFDVELAFTRRVPHVAVLRAGYFMENFLHDIPSIVAGT
                     VYSGVNPDRRFPLVATRDIAYKAAAFFLSQDWIGHKVVGVHGPSHISMRQAYAQIAEV
                     VGRPINLVPVPVDAVRSAMLGMGIPEFVVSGYCEMFDAFNSDKPDFCEPRTVETTTHT
                     TLRSFARKVLGPALAHAGKAHS"
     gene            15990..18731
                     /locus_tag="PKL17_10870"
     CDS             15990..18731
                     /locus_tag="PKL17_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45278.1"
                     /translation="MTSKNPYRTAGSGGVYVEREADIRLRRELSDNRRFPLIAAPQES
                     GKTCLIRHTIDSLDPAGFCVLSVDLSRLRLGSPTQFMGELLGAIAKEGDFDQREILPD
                     NPEDTVLAWLGTFPQRLVLFLDGCEVLERPEICEAMFGKLRLLYNIRNENEEFSRLQI
                     FLAGAVVGRKLIPIHLQAPFGIGNEISLRPFNAEQVDALAWHLSTSGVSVSNDIGSRL
                     THHSGGLPFLCQQVLSSLWEDAVSRGSPIATADVDRAVEDLIERAPQIEHFANIFRIY
                     SSDPDLLDSFHRLCRGQTLSEEMIESLTLTGLCEEDLPFRSSIYERVFAEGGPLALPP
                     PVGQRPTRQVAIIAGPPSEPRPAREDSEAIEVNPDEDSGFMVLSEPPPPRPQPAPVQS
                     PTELPSAAESVAAASQVAAQNAQITTEVPPVSAATPAYGAATPSSQSATPSSRTPTYA
                     ASLGVPSLPPAAPSTRTPTYLGGTPSTPAKSERTPTYAGQLAVSQSPSASTPKYPGAT
                     GSMPALPDGGAGSGMPASNKTPMYNLQMTPPIPSQRTPTYPAPGMPLGDMGAMSQPPA
                     QAMAGDSIDDQPTRLAPAAPRSATQELREQIDKQILLSAELDLFCNTYFPHVARLFNS
                     PLSRAEKTDLLLAHAEHTSLRDRLREWSKQRREDRPEAYSNTSTESIEPLAVDAVEVK
                     SAPSVPPASPPPVPPGPVTLKAEVPASLVAQAQPTGQLEIKQATGEGGEGFKPPATAS
                     LQVGIGLVLANRYFLTSEIGPGAVATMWNAYDRIKDEQVAIKILHGAAADNPQLVERF
                     WRSAQQMSGLSHPTIVSVLNKPREENAIHYVVIELQSGGNLRQWVQGGKLTRMQLMRT
                     LQRLGSALGFAHDKKVFHRDIKPTNVFVRRDLVARFSGLRRRGAGKWGLACAASQTF"
     gene            complement(18760..>19104)
                     /locus_tag="PKL17_10875"
     CDS             complement(18760..>19104)
                     /locus_tag="PKL17_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45279.1"
                     /translation="FRVRALAWLARIEQQRGNIDAAALAAEASLAQLQVLGRAGTDEG
                     LVYHTVANIKLAIGQNETAKALLAEGRTRLLRQAAAITRSEARNDFLYKLTENAELLA
                     LAAAHCPEPLPS"
BASE COUNT         3662 a         5663 c         6009 g         3770 t
ORIGIN      
        1 catgacggcg acgccgccgc ggctgctcca gcgctacaac gaccccatga ttccgcccgc
       61 gctcgacgac ttggtggcac ggcttctgct caaagaaccg gccaagcgtc cctctgccgc
      121 cgagacctcg cgcgagcttg agcagatcct gagcaatctg tacgacagcg cgcgctctcc
      181 gcagacgctg catgatcagg cgacccaaga gctgccgctg cttcatcgtg ccacccaggt
      241 tgtcggcgcg gtggaacgca agaccatggt ggtggagcga ccggaccggc ctacccaggt
      301 gctgggtcgc gacaactggc ccacccagct tttgccccag gtggtgacgg aagccaaggc
      361 ggaagcgcag gccgtcgccg atgcagaagc ctatgcgcgg ctggccaagg aactgttcgc
      421 gcaggcggtg gagctggctg accgcatctg taccggatcg tggccgtccg cgctcaagaa
      481 cctcggcagc aaggtcgccg actgttacaa acaagaagtt gagctggccg aaaagctctt
      541 ttcgctcgaa aaacttttgg ccgacaaggc cgcggcttcc ggtcagcaca acgcgctccg
      601 ccagcaaatc ttggcgctgt cagagcagct acaagccgac gcggcggtca acgaagacga
      661 ccgcaagcgg ctgacggcgg agctggagca aaaagagcgg gcctttttcg atgcggaagc
      721 ccgctggcga aggctgccgc cgacatcacc tgattcgctg cggcggcgga ttgccgacgt
      781 gcgccgcgag cgtgttcgcg cgcagattct gtttgttcgc ctgattgtcg agatgccaac
      841 agcgggtgct ttgcttgccc agcgcgatca gcttgcggcg cgattgttcg atatcgacaa
      901 cccgaagcgg tccggtcccc aaggggctgt gaccaaataa ccgcagggcg tctgatgtcg
      961 agtctgtact tgcttccgct tgcgctgtcg ctgcttgtcg acccgcagct tgccaccgtg
     1021 gcagccgttc ccgcggccca ggtcgtcgaa aaagacctgg ctccatcgtc gcgttttgcg
     1081 atttcgcctt atgtacaaga cgtccaaaaa gactcggcgg tgattgcgtt tgcgactgat
     1141 gcaccgagca tggccttggt tcatttcgac ggacccggtg ggcgtggcgc cgtgatgtct
     1201 ggcccgcaac agcaccacaa agtgcggctg acgggccttt cggctggcac aagataccgg
     1261 tacaccgtcg cggtgcgccg acaaggcgac ctgaccggcg ttgagtcgat gagcgagtcg
     1321 gctgagtttg cgacgatggc cgaaggtctg ccgtttctgt tcctggtgta cggtgactgt
     1381 cgagaccgcg attccgacca ccaagcggtg gtgcgcgcga tgctaaaaga gcgtcccgac
     1441 tttgtcctgc aaaccggcga catggtgagc cgggccggtg atgtcggcca gtggcggcgc
     1501 tactttgcgg cagtttctcc gctgattcga tcggtgccga tgtatccggc gcttggcaac
     1561 catgagctgc gcggcgaccc cgatgcatca cacttttttc gctactttgt catccctggc
     1621 gatcgcaagc gacgtccggt ctactacgcc tttcgctatg gtaactcgct gtttgtttcg
     1681 cttgatggca acagtcccct ggatcacgac cagtccgtct ggctagagcg cacgctcgcc
     1741 gaagccagcc aagatcgcag catttcgcac atgtttgcgt ttgtgcacca gccgccctat
     1801 tccgttggtg cttactgcgg cagtgatcgc attcagcggc gacttgttcc gatccttcgg
     1861 cgctatgggg tccgcgccgt ttttgcagga catgagcatg cgtaccagca tctagagcgc
     1921 ggcggacttc gctactttgt gacaggcggt gggggcgcgc cgctttatac ccgcagtacg
     1981 tcgtgcaatt tcgaggacga catggcgctg cggttgtttc gtgccgagca tcactatctc
     2041 cgcatccagg tagatggtga tgccgccacg ctgatggcgg tcagcaaaga tggcgaagtc
     2101 atggaacggg tgcttctgca tgaacccgtc gttcccgata gcgactatcg cgtgggcccg
     2161 ccggtcttgt cgcagtcccg tgtgccgcca tcgccgcccg ttggggcaag cagtccaggc
     2221 caacccagcc ggacaaggcc gagcgcgccg ctgtttctgc ttctgacaag cgctctggcg
     2281 atggtggttg ggctgctttt gctaacagcg ccgcggtcgc gtcgacgggt cgatctgatg
     2341 ccggacgcgc ggcggcgatc gtgatgacgc cacaaggggt ccgctaggcg tcaggtcgtg
     2401 ccgggcacct gagtcaagcc cgctgtcttc ttttctgtgc cgcttggccc gcaggccagc
     2461 actgccttgg cgtgttttat cgggcggatc tgggtgcggc cagcggtcga ttttgcaaaa
     2521 cagcttgggc cgcagcgttg gccacgccgt gattttgcaa aagcgcgcgg tcccgtttct
     2581 gtgaccctct tcctctctgc gttttcggca cggcccgctt ctgcttggcc ctgtttggtt
     2641 gttgtgggat ctgtggccgc gccgcggctt gctgcggaaa cgtgcaaaaa aacggcggcc
     2701 atcgggtccg ctgctaccgc aatttgtcca aatcctttgg tccctgcgaa cagcgcccac
     2761 cgacaaaaaa ccaattcatt tgagactacg acccaaagcg gtgtatacca cgccgtctga
     2821 ccggcccgcc ggtcattccc attcccgaag gagtacttct gcatgtcgcg gaaaaagacc
     2881 gtcgccttct gtgttctcgg cggcacggcc ctgtctgtca ttgcgatgag ctgcaccgac
     2941 tacttgaccg gccaaacgcc cgagcctgat ggtccgttgc tggttaccaa gctcactttg
     3001 ctcgacaagg tatcgcgcga caaagcggtc tttaccgaca ccagcttgcc aaactgtatg
     3061 cagtatccgg cggacgtctg ctcgcaagac gcgaaccgga actcgtttgc ctgccggact
     3121 tgctataacg acgtgctcaa agacacctac tcgctggcca agagcccgcc gacgcctgat
     3181 tcggcgacgg atctgcgggt ggtctttaac aagattccgc gcaagtggaa tggcagcgcc
     3241 ttaaatcccg atgacgttcc ggacatggcg cttcggcttc agtgcggcgg cacttgccag
     3301 ggcattccga cgtacaagcg gctgctcgac atctcgggat catcgctctc gtatgatccg
     3361 acggacattc cctacggccc ctcgctgcgc atcgcagttg accgctcggt ggacccgcgg
     3421 gtcgcgctgg aaccgggggc ttcgtactcg gtgttcgtcg atgtggggct ttccgatcga
     3481 agcgacaacc gactcgacgg tgcgcaagcc caagccctgc tttcgtttaa gaccgagcca
     3541 cttacggtcc tgcaaatggg acgtggtgac agcaaggccg acccgtatgt ctacgcatcg
     3601 acgaaatatg gcgatggcgg aacgggaaat ccgtacagca ttgcggatct gccgcgcaac
     3661 agcgccatcg tgctcaagct aaacgcttcg attcatcccg acgcgctcgg gccgctcaag
     3721 ctgacgggat catttaagcg ggcagacggt tcgactggcg cggtcgatgt gctggttggc
     3781 accaacatct gggccaccgg cacagacggc agtgtgaccc gtgacccaaa gacgatgggc
     3841 tgcatccagc agaaccagcg tcttttggcg atgtggccgg acaccccgga cctcaagtgg
     3901 ccaatgggcg cagtggaagt gacgtttacg ctgacgggtg gaacggtcaa agacgtcgcc
     3961 cagaaggccg gcttcccgat gggtgttcac gcgcttgcgg gcgatgtgac ggtgcgtgcg
     4021 cggcttacca cggcggatgc gccgtcgggc tataccggac ttacgacgca gcgcgcgaag
     4081 gtttccgcgg gcgcttgccc acagcttggc ggggccgata tggcaacgct gccggatatg
     4141 gcggtgagcg acatgaccgc caaggacatg tagcgaggga cttacacggc tatgaagcga
     4201 actgccaaaa atcgtcgttt ccgtttgcgt ctgccgctca ccttggctct cggtggtgtg
     4261 tgcgcggctc cttttgttcc tggcgtcgcg catgcccagc ttcagcttgg gcaaatccaa
     4321 ggcgtcatca caaaccaaga caacggcaag ccgctgcaag gcgtcaccat cgttgtctcg
     4381 ggcccggctc ttcaaggcga gcagtccgaa atcaccgaca agtccggtag ctacctgatc
     4441 acgcagctcc cgccgggcga tggctacatc atccgttttt acttcggtga tgtggtggtc
     4501 gaacgcccag gcgtccggat ttcccagagc aagacgctga cggtcagcct cagcatgccg
     4561 acgcagaagg gcaagaacgt ctacgtcgtc aaggaacggg caccggcagt tgatacggcg
     4621 acggccaaca ccggcgttga aatcaaccaa gagatcctgc aaaacaccgc cgtccggggc
     4681 cgcacgtttg aatcggcgat gagcctggcc cccggttctg ccgacgtggc accgcgttcc
     4741 caagccggtg gtgacgttgg tgtcagtttt gcgggtggta cgggcaacga aaacgcggtt
     4801 ctggtcgacg gtctcaacac caccgacctg ggcacgggtg tcgttgctac ccagcttcac
     4861 cagtacttca tcaaagacct gaacatcatc acgggcggtt atcaggccga gtatggccgc
     4921 gcaaccggcg gtgtggtgtc aatcgtcacc aagacgggct caaacgagtt ccacggcggt
     4981 gtcttcggtt cctggtcgcc gtatcaggcg acgccgacga cggttgcccg gctgggtgaa
     5041 gcgctggcgt tccgcagcaa gcagtttcag cagtacgact ttggctttga gctgggcggt
     5101 ccaattctca aagatcggat ctggttttac gttggttttg cgccgacgtt caccatcaac
     5161 cgcaccgagc gggtggtgcg cacccaagtc aagtcccagg gcaagcggcc cgacggatcg
     5221 tggaaggcgg aaatcgatcc cgattaccgc gaccccgatt acctggcatc ggacatcctg
     5281 tatgcgccgc tccgttcgct ggcgctgcgg accgaagaag ttccagaaga gagccgtctg
     5341 ctcgacgagt acgcgcgact ctacaactgg attgccaagc tgcagtttaa cctaagcccc
     5401 gaccacaaca tcacgctcgg ctatatcggg gcgccagagt ttcgcagcga atacgacggc
     5461 tacgcgagcg acaccgacgc gaccaagatc gaccgctcga agcagattca cgacgcgacg
     5521 gttcactacg tcggcaagct gttcgaccgc aagctccaga tcgacgtgat ctacggctac
     5581 cactaccagg ggctacagga gctgccgaac gccgaaaaca agcagtattt ccgctatcgc
     5641 tcgccggcca acgacgactt gcgcggggat ctggcgtttt cgctcggtga ctttgaaaac
     5701 atcagccgct gccgggtcga gcttgatccg acgacgcgca agacgctgtg gaatccctgc
     5761 ccggtcacgt cgtatgtcaa aaacggctac ggtcagtaca agccaaacca agtgctccag
     5821 cggcacgcgg tgcaggcgtc gggcacgttc tacggcaagc tgcttggcac gcatggcata
     5881 aagctcggct tcgactttga agacaacctg agcgacaaca cccgcaaata ctctggcgtc
     5941 gactttgatc caagtgatcc ggcgtcgggc agcttcaatg gccgtctggc gtatgagacg
     6001 gacgcttctg gcaccggcgt gcggattcgt cgcggctttg gcacattcga gccatttaac
     6061 cgctttggcg atgcgggcgt tccgtgcggc gtcaacaaag accgctactg ctacaactcg
     6121 ttccgcgccg tgaccgagac gcgcaactat gcgctgtatc tgcgcgatca gtggagcgtc
     6181 gccaacgtcg gactggtgct caacgccggc attcgctggg aagcgcagga aattttcggc
     6241 gctgacggca gcaagcagat tgcgatttac gacaacgtcg caccgcgggt gggcgcgacg
     6301 tgggacccga cacgcaaagg ccgggccaag atttacgccc agtacggtcg gttctatcag
     6361 tcgattccga tggacatcaa cgaccgctcg ttcagcggcg aaggtctgct cgtcggaagc
     6421 tcgtttacca aagactgcgc caaggaatcg atctcgccga ccggaattcc cgtggtggtc
     6481 gccaaaggcg cgccgggttc gccctgctcg ctgtctgatc cgcgcgtatc gggtggtggc
     6541 gtgtttgcac cggttgcgcc gtatctgaag ggtcagttcc tcgacgaagt ggtcggtggt
     6601 gcccagtatg acctggggct cgacattgtc gtcggtgcgt actacacgta tcgctggctc
     6661 ggcagcatcg ttgaggatct gtcggtcaac ggcggcaaca cgttctttat cgccaatccg
     6721 ggtgcggaac ctgatcccaa agtcgcggcg gatcttcaga ccgaagccga ccgactgaag
     6781 cgggatgctt cggcagcccc gaccgatgcc aagctgcaaa aagatgccga catggcccag
     6841 cgacgcgccg aggtctacaa agcgcaggcg ctgttcccca aggcgacgcg cgactatcac
     6901 gcgatcacgc tgactttgca gaagcggctg tcgaatcggt tttcgatcct ggccaactac
     6961 acctactcgc ggctgctggg taactatccg ggtccgttta gcccgtacaa taaccagctc
     7021 gacccgaaca tcagcgtcca gtacgacatc atcgacttga cggtgaaccg caacggaccg
     7081 ctcaacaacg accgtccgca caacttcaaa gcgacggggt ttttccagca gccggtgttt
     7141 ggtggctcgg gcacgattgt cgccagcttg accttcacgg cgatatcggg aagaccgatt
     7201 caggtgctcg ggcagcacaa cgcttatggt gcccgcaata ccttcatcct gccgtcggga
     7261 agtgccgggc ggacgccgac gattacccag cttgatgcgc acctggccta cgagcaccag
     7321 ctcaccaaag aggtcaagct gtcggtctac ggcgacgtgg tgaacttggc caaccagcgc
     7381 gcggtcgcaa acgtcgatga cgagtacacc ttttcggtgg tcaatccgat tgccgggggc
     7441 aaggtcgagg atctgccggc gctgcggacg cttgatgggc aaaagccaat cctcaactcg
     7501 aactatggtc agccgaccgc ctaccagtca ccgctgtatc tccgcttcgg cgcgagactc
     7561 tctttctaaa tgaaaaagct gattggctcc gtcatcgcgt cttgcgcggt ggcggtggtg
     7621 tcgcttgggg cggtggccag ggcgcaggtc gtcgcgtttg atccggccag cctgaccccg
     7681 tattttgcgt cggggccgct tctcaaagcg agcgagcgac tgcgcgcggg ggatgcgacg
     7741 ggtgcgcggg ctctgttcac cgactatctg acagcttggc ggcaagagca gacggccagc
     7801 aaaaaggacg cgcgcgacga cgcaatggat gtgcgggtgc ggtatctgct cgggctttcg
     7861 cttttgcgta ccgccgagca gctaccccct ggccccgaga gggtggctac tgcaacgggt
     7921 gcggcgcagc actttttggc gctttcgaag agctatccgc tcttggcgag ctatcacgcg
     7981 ctgtacgggg cgcagtcgct gcttttggca gggcgtccca aagacagctt gacgcagctt
     8041 gcggctgtgc cgacggactc ggtgctcgac tgcgaagcgc gctttctgcg tggcgatgcg
     8101 ctgctgcggg cggcggagct tgagccgaag tcgcaggccg cgctgcgcga gcaggcggtc
     8161 ttggcgttcc gggcgtatct gtcggcatgt agcgacgatg gcgcgcgcaa gctgcgggcg
     8221 caggtgcaga ccgcgcagat actcgacacc ctgtcgcgga cggcagaagc gctgcaaatt
     8281 tggcgacggc tttacatcga agcgccgacc gagtccgccg gggttttggc cggcaaaagg
     8341 ctggaacagc caggggtcaa gcagccgccg ttttcggcca gtgagctttt ggaccgtgcg
     8401 caggtgctgt tcgatgagat gcgcaacccc gaaagcgaag cggcgttccg tttggtgttg
     8461 gagcagccgg aactcgacga caggcagaag tgcattgcgc gctatcacct ggcgcagagc
     8521 gtgttcaagc aaaggcagcg tccgcgggcg gcaccccttt tcgacgatgc ggtggcggcg
     8581 tgtgcaccgg ctggggcgaa aaacgacgac ctgcacatga agtcgttgta ccaaggggca
     8641 cgctgtcatg cctcggcggg acggttgcaa aaaggggcgg atctgttcgc agaggccgag
     8701 gcggcgcatc cggggcacag ctacgccgac gattcgcgac tgcgacaggc cgagatgtac
     8761 ctggacctgg ccgaaaagct gggtagagat ggcgcgaaaa agacctgcga cgccgagcag
     8821 tgtcccgact atgaagcgaa gtttgagtcg ctgctgtccg acctgccaac gcgatatccc
     8881 gatggcgacc gccgggccga agcactgtgg cgactggcgc tgcgggcgct gcgcaaaaaa
     8941 gatccgtcca aagcaaagac ctggctgctc ggctcgctgg agaaaatccc gcgcgaagtt
     9001 ggctgggatc aggaaggccg gacgctgtac tggctgggac gactggccga acagtcggac
     9061 gaaaaaaccg cggcgagcga ttactaccaa aagacggcgc ggacctatcc gctgtccttt
     9121 tatgcgctgc ttgcgctaaa ccgtttgcgc gacggctttg cgacggaatt cgatgcactt
     9181 ctgcgcgagc tgtatggcga gccagatgaa gtcgaaaagg cgccacccct tacgtttttg
     9241 ccgcgggctc tgtacggcat gccgggcttt ctgcgcgggc tggagctttt gcggctggga
     9301 cttggggcgt cagcgcggcg agagtttgca gccgtcggaa tcgtggtgcc aaagagcaag
     9361 gacgcgctcg ctggcaaaga gggcgatgac gcgcagaccg agcgactgtg gctggcttcg
     9421 gtgctgtatg agcgtgcagg cgactggcac cgctcgcact tcattcctcg ccacatcctg
     9481 accgaatggc agaagcacta tcccaagggt ccgtggcggg cgtactggct gcttggctat
     9541 ccgcggggct acgcggaact tctgaccgcg gcggcgcagc agaacggaca tccggaagcg
     9601 ctgcagtttg cgatcgtgcg ggaagagtcg gcctttgatc cgctgaccga gtcgtttgcc
     9661 aatgcgatcg ggctgacgca gatgattccg ccgacggcga agcgtttttc aggtgggctg
     9721 cccttcgatc gcgaagcgct gcgtgatccg gcaaccaacg tggcgatcgg aagtcgcttc
     9781 cttggctttt tgtggcagac gatgaatcac catccgtcgc tgaccatcag tggttacaac
     9841 gccggggagg gatcggtgtt ccgctggctg cgaaactacg ccgatctgaa agaaatcgat
     9901 ctgtttgtcg aagcgattcc ctacgacgaa acgcgcggct acaccaagcg cgtgctttcc
     9961 agttacctca cgtatagttg gcttgcgcca cttcccgccg gtacacctct ttcgcagcgg
    10021 gttcccgtcc tgccattttt gctgccgcca ccaggcagca aaggcaagct gagcgacgca
    10081 aatcctgccc aggcagcgcc cgcaaaccgg gaaaaaccga atccgtaaat ggcatttggg
    10141 gacgacatcc gtccgatcgc tctcgctttt caagccaccg aaactcctgc caaaaaaagc
    10201 gcgaggcggc agttgatttg gtaactgggt tcggtaaaac tcaaaaaagt agaagcgtaa
    10261 gattcacacg ccatacgtat cggaagatgc cgcaaaagaa cgaagaacct gacggtcatt
    10321 ctgaaagttc tcaaaagggg tcactggacg aatcgagtga ggagtccagc ggcccgaaac
    10381 ccaccgcgcc gttgacccaa tcgctccttc aggtgattga acagcggcgc agtgaagcac
    10441 cttcaccgca aaaagcgcgc ttcggatttg gccaactgct tttgtcagtc tggcgctcgc
    10501 ttccgtgcta ctcccacggc ccggttcagc acccacgggg accgcttatg accggactcc
    10561 gtctcggagc cagctacttc caggtccata aaaaagaggt tgcatgaaga tcgcgttcac
    10621 ctacaacctc cgcttaagcg acgcggagga agaggccgag tttgactcac ccgagactgt
    10681 tgaagccatt gccagcgcgc tgactgtcgc tgggcatgaa gtccagaaga tcgaagtatc
    10741 gggtccggct tcgcatttgg ccgcccggct ggaggctttt tctcccgatc tgatcttcaa
    10801 cgtcgcggaa gggaagcgcg gtcgcgcccg tgaagctttt tatccggcgc tgtttgagga
    10861 gctgggctat ccgtacaccg gatcggatgc gcacacgctg acggttacgc tcgacaagtg
    10921 gctgaccaag cgcgttcttg cgtcccatgg catggacacg ccacgcggtc ggctgttcac
    10981 ccatcgcgat ctggctgaag tgaacaagct cgacggattt ttgggcggat cactcggggt
    11041 gccgattcct gcgatcgtga aaccgaactt cgagggttca tccaaaggaa tcagcgatga
    11101 gtcggtgacg cgcgactgga agagtctgcg cacagtggtc accaagatgc tggaacagta
    11161 cccgcttggt gtgctggtcg aagagttcat cgcgggcatt gatgtcacgg ttccttatat
    11221 agaaggagtc ggtgatccag cgggggaagg aatccttcag ccggtggagt tttcggtcga
    11281 tccgcaggtc cggtccaagt acaacctgct cgactatcgg ctcaagaacc agaagtcaga
    11341 caaagtccgg ctgcgctgtc cggcagacct tccgcgtgat gtgattgcgc ggctgcgtgt
    11401 actggccaag caggtcgtga agcttttgtc gatccgtgat gtcgcgcgga ttgacttccg
    11461 cttggccgaa gatggccgga tctatttcct ggaagtcaat gcgctgcctg cgctcgatcc
    11521 ggcggcgagc ttctttgtcg cggcccgtcg cgagggactc agcttcggtg acaccatcaa
    11581 tgcggtgctg tcgagcgcgg cggttcgctg gaagcttctg ccttcggtga gtgccaagcc
    11641 caaggccagc ctgaagggcg aaaaactgcg cgtcggcttt acctacaacg tcaagcgtcc
    11701 gggcggcaaa gccctgggcg atgccgaggc cgaatacgat tcaccggcga cgattgcggc
    11761 gattacttcg gcgctcgagt cgctcggtca tgaagtggtg cagctggaag cgaacggcga
    11821 gctgcccacc cggctcaaag atgcgcaggt cgatctggtc tttaacatcg ccgaaggtgt
    11881 gtcgggtcga aaccgcgaag cgcaggtgcc cgcgctgtgt gagctgtgcg ggattccgta
    11941 caccggcagt gatgcagcga cgctgtcttt ggcgctcgac aaggcgctga ccaagcggct
    12001 gctgaagcag cgcaacatca atacgccgga atttcaggtc ttccacacgg ggcgcgagaa
    12061 gatttcgccg acgctgcgct tcccacttat cgtcaagccg attgcggaag gttccagcaa
    12121 aggaatcgcc ggcaaaaaga gcgtggtcga tgacgtggcg acgctgcgca aagtggtgct
    12181 ggagctgatc cagaagtacc gccaaccggc cctggtggaa gagtacgtca ccgggcgcga
    12241 gtttacggtc ggactgctgg gcgagcgtcg tccgcgcatc ctgccgccga tggagatcgt
    12301 gtttaacgat cacagcgaca agcgcccggt ctacgactac acgtacaagc aggactggga
    12361 ctcgcgggtt tcgtatcagt gtccggcgaa gctttcgccc aaggaactga aggcggtgga
    12421 aaaggccgcg ctcgatacgt ttctggcgct ggactgccgc gatgtcggtc gcgtcgatct
    12481 gcggatggat gcgcacggca atgtctacgt gctggagcta aatccactgc cggggcttac
    12541 gccggactgg tcggatctgg tgctgatcgg caaagcgatc aacatggact accggaccct
    12601 gattgccgag atcatggccg ggggtcttaa gcgactgcgc gaaaagaaac gcggcgcgct
    12661 tccacctcgc aacggcaaga gcaacgaatc caagagcagc gtgtcgagtc cgtcaacggg
    12721 agtgcgcggc gaagatggtg acggcgcagc cccggctgca gagcacacgt tgccggcggc
    12781 ccatgcggtc gctggcgaaa aagccgcagc cagtcccaag tcgcgtgcca aaggatcgtc
    12841 ccgccgccgt cccgccgctg cttctcacta gtcgcttggg cgatgtcgct gctttccgat
    12901 accgatctgt atctgtttaa cgaaggtcgt cacctgcgtc tgtacgaccg gctcggtgcg
    12961 catcttggca ctgcgctggc cgatgggacg gaggtgtccg gttgtcactt tgcggtgtgg
    13021 gctcccaatg ccgcaaaggt gtctgtcatt ggcgactgga atgactggaa tccggaacgg
    13081 aatccgctca cggtggttgg ggcgtccgga attcactacg gctttgcaaa agatgtaaaa
    13141 gaggggcagc gctacgtcta ccaaatcgtc agtcagcacg gcgcgtacac tgtcgacaag
    13201 gccgatccgg ttggattttc cgctgaagtg tccccggcca aagcatcggt ggtgacgcgg
    13261 cttgactatg actgggggga tgcggactgg ctgcaacagc ggcgcaagaa aaacgcgctc
    13321 tctgccccgc agtcgatcta tgaactgcac ctgggatcgt ggcagcgcgg ggaaggcggg
    13381 cgattccttt cctaccggga agtggcgccg cggctctgtg aatatgtcaa gcggatgggc
    13441 tttacccacg tcgagcttct gccgatcatg gaacatccgt tttacggatc gtggggctat
    13501 cagtgcaccg gcttttttgc gcccaccagc cgctacggat caccgcagga ctttatgttt
    13561 cttgtcgatt cgctgcacca agcaggaatc ggtgtgatct tggactgggt tccttcgcac
    13621 ttcccagcgg attcccatgg actgggattt ttcgacggaa cccacctgta tgaacatgct
    13681 gatccacgcc agggatttca tcccgagtgg cacagtcttt tgttcaacta cggacggaac
    13741 gaagtccgct cgtttctgct ttccagcgcg ctgttctggc tggaccgcta ccacattgat
    13801 ggactgcggg ttgatgcggt cgcgtcgatg ctgtatctgg attattcgcg caaggaaggg
    13861 gagtggattc ccaatcgcca aggtggaaaa gaaaacctcg atgcgatttc gtttctgcgt
    13921 cagctttcgg aagatgtcta ccgcgagttt cctgattgcg aaacgattgc agaggagtca
    13981 acttcctggc cgctggtgtc gcgtccgacc tctttgggcg gtcttggttt tggtctgaag
    14041 tgggacatgg ggtggatgca cgacacgctg aaatactttg cgctcgaccc cgtgcatcgc
    14101 aagtaccacc acaaccttct gacgtttcgt ggaatgtatg cgcagagcga aaacttcgtt
    14161 ctgtctttgt cccacgatga agtcgtccat ctgaagggct ctctgctcac caagatgggg
    14221 ggcgcttcgg cggacgactg gcagaagcgc gcgagcttgc ggcttctgtt tggctatcag
    14281 tacgcgctgc ccggaaaaaa gctgctgttc atgggcgcag agcttggcca gtggcgggaa
    14341 tggaaccacg aagggagtct ggactggttc ctgctcgaca cgccggaaca cctgggaatc
    14401 cagcggtttt tggcggatct gaaccgcgtc tattccgccg agccggggct ttctgcgcgg
    14461 gacttttccg aagacggctt tgtctggctg gaagcggacg accaagacca caatgtgctg
    14521 tcgtttttgc gccaaggtga aacgccagaa gaaacggtgc tggtggtctg taacttcgcc
    14581 aaggtgccgt gggaagggta tctgcttggc gttccctgcg cgggttattg gcaggaactt
    14641 ctgaacagtg atgcgtctgt ttacggtggc agcggaggcg gaaatctggg cggttgccag
    14701 tcggaatctg tgccggcgca gggtcagccg cacgccatcc gcgcgtttgt gccgccgctt
    14761 gctgtgctgt acttccgtcg tgtaaagtaa cgccgcacca aactgccctt gtggcatcgg
    14821 ccctttaccg gaaggaacac acgcaatgag catcgtgata aatacgccga gtggcaacat
    14881 cggacgcgcg cttgcaaacc tgctgctgga tgcgggcgag caggtcactg tcatcagccg
    14941 ccatcccgac aaagtggcgg atctgaccca gcgcggggcg cgtcttgtgg tcggctcgat
    15001 cgatgacaaa gacacactgc gtgaggcctt tgcaggtcag aagcaggtgt tctgggtgac
    15061 gccgccgcca gcgcgtccgg actttgccga ttggaacggt gccacggcgc ggctggctgc
    15121 ggaagcggcg cgggcagccg gtgtacagcg cgcggtcatt ctgagcagct tccttgcgga
    15181 ttctggtcct ggcgcgggtc cgatttccac gctgttcgat gtggaattgg cattcacgcg
    15241 ccgcgttccc catgtggcag tgctgcgtgc gggctacttt atggaaaact ttttgcacga
    15301 catcccaagc atcgtggcgg gaacggtcta ttccggtgtg aatcccgatc gccgtttccc
    15361 actggtggcg acgcgcgaca ttgcgtacaa agccgcggca ttctttcttt cgcaggactg
    15421 gatcggtcac aaagtagtcg gggtacacgg cccttcgcac atcagcatgc ggcaggccta
    15481 cgcgcagatt gcggaagtgg tgggtcgtcc gataaacctt gtgccggtgc cggtcgatgc
    15541 ggtgcgcagc gcaatgttgg gaatgggaat acccgagttt gtcgtcagcg gctactgcga
    15601 gatgtttgac gctttcaaca gtgacaagcc ggacttctgc gagccgcgga cggttgaaac
    15661 gacgacccac acgacgctgc gttcatttgc gcgcaaggta cttgggccgg cgcttgcgca
    15721 tgcaggcaaa gcacattcgt aagtccttgt gatcttggcg ctttttcctg ggcgcaaaat
    15781 ccacctacat tcccaccgct tgggaatgcg ctcctgcctc gcgcacccaa aattgttagg
    15841 ggcgcgacac cctttgatct agcggcaaaa actagatatt ctgatcatat gccgctctgt
    15901 acgcagtgga cgcgcccgct ccctatgggg aaggttgcgt cttcatttcg atgcggtggc
    15961 gcggcacaga gggacaggag acgcaagctg tgacgtcgaa gaacccctat cgcaccgcgg
    16021 gatcgggcgg tgtgtacgtc gagcgtgaag ccgacattcg tctgcgccgt gagctttcgg
    16081 acaaccggcg ctttccgctt atcgcggccc cgcaggagag cggaaaaacg tgtcttatca
    16141 gacacaccat cgactcgctc gaccctgccg ggttctgcgt gctttcggtg gacctgtcgc
    16201 ggcttcgact gggatcgccg acgcagttta tgggtgaact gctgggcgcg attgccaagg
    16261 agggagactt cgatcagcgc gaaatccttc cggacaatcc cgaagacacc gtcttggcgt
    16321 ggctgggcac ttttccgcag cggctcgtgc tgttcctcga cggctgcgaa gtcctggaac
    16381 gtcccgaaat ctgcgaagcg atgtttggga agctgcggct tctttacaac atccgcaatg
    16441 aaaacgaaga gttttcgcgg ctccagattt tcctggccgg cgccgtggtg ggacggaaac
    16501 tgattccgat tcacctgcaa gcgccgtttg gcatcggcaa cgaaatctcg ctgcgaccgt
    16561 ttaacgccga gcaagtggac gcgctggcgt ggcacctgtc gacatccggc gtaagcgtga
    16621 gcaacgacat cggaagtcgg ctgacccatc acagcggcgg tctaccgttt ctgtgtcagc
    16681 aggtgctttc gtcgctatgg gaagacgcgg tcagccgtgg ttcaccgatt gcgaccgcgg
    16741 acgtcgatcg tgcagtcgaa gacctgatcg aacgggcgcc gcagatcgaa cactttgcca
    16801 acatctttcg catctatagt agcgatcccg atcttctgga ttcgttccat cgcctgtgcc
    16861 gcggacagac tctttcagag gaaatgatcg aaagtctgac gctgaccggc ctgtgcgaag
    16921 aagacttgcc gttccgttcc agcatctatg agcgtgtatt tgccgagggc ggtccgcttg
    16981 cactgccgcc ccctgtgggt cagcgcccga cgcgccaagt cgccatcatc gccggaccgc
    17041 cgagtgagcc gcgacctgcg cgagaagact ccgaggcgat cgaagtcaat cccgatgaag
    17101 actctggctt catggtgcta agcgagccgc cgccgccaag gccccagcct gcgccggtcc
    17161 agagcccgac cgagctgccc agcgcggctg aaagcgtcgc agcggcgtcc caagtggctg
    17221 cgcaaaatgc ccaaatcacg accgaagtgc cgccggtcag cgcggcgacg ccggcctatg
    17281 gcgcagcgac accgagttcc caatcggcga cgccaagcag tcgcaccccg acctatgcag
    17341 ccagcttggg ggtgccatcg ttgccgccag ccgcgccgag tacgcggacg ccgacgtatc
    17401 ttggcggaac cccgagcacg cctgcaaaaa gcgagcggac gccgacctat gcgggtcagc
    17461 ttgcggtcag ccagtcgccg agtgccagca cgccgaagta tccgggggca acgggcagca
    17521 tgccggctct gccggatggt ggtgctgggt cgggcatgcc ggccagcaac aagacgccca
    17581 tgtacaactt gcagatgacg ccgccgattc cgagccagcg gacgccgacg tatcctgcac
    17641 cgggcatgcc gctcggagac atgggggcga tgagtcagcc gccagcgcaa gcgatggccg
    17701 gcgacagcat cgacgatcag cccacgcggc tggctccggc tgcgccgcgt tcggcgaccc
    17761 aggagctgcg cgaacagatc gacaagcaga tccttttgtc ggcggagctg gatcttttct
    17821 gcaacacgta ctttccgcat gtcgcgcggc ttttcaacag tccgctgtcg cgcgcggaaa
    17881 agaccgacct tttgctggct catgccgagc acacgtcgct gcgggatcgg ctgcgtgagt
    17941 ggagcaaaca gcggcgggaa gatcggcccg aagcgtactc gaacacgtcg accgaatcga
    18001 tcgagccgct ggccgtggat gctgtggaag tcaagtcggc gccgagcgtg ccacccgctt
    18061 cgccaccgcc ggtgccgccg gggcctgtga cgctcaaagc ggaagtgcca gcgagcctgg
    18121 tggcgcaagc gcagccgacc ggccagttgg aaatcaagca ggcaacgggt gaagggggcg
    18181 agggcttcaa gccgcccgcg accgcttcgc ttcaggtcgg catcggtctt gtgctggcca
    18241 accgctactt cttgaccagc gagattggtc ctggggcggt ggcgacgatg tggaacgcct
    18301 acgaccgcat caaagacgag caggtggcga tcaagatcct gcacggcgcg gcagcggaca
    18361 atccgcagct cgtcgagcgc ttctggcgca gcgcccagca gatgagcgga ctttcgcacc
    18421 cgacgatcgt cagcgtgctg aacaagccgc gcgaagaaaa cgccatccac tacgtggtga
    18481 tcgagcttca atcgggcggg aacctgcgcc agtgggtgca aggcggcaag ctgacacgca
    18541 tgcagcttat gcgcacgctc cagcgactcg gatcggcgct gggctttgcg cacgacaaga
    18601 aagtctttca ccgcgacatt aagccgacca acgtatttgt gcgacgtgat ttggtggcgc
    18661 ggttttccgg tttgcgtcgg cgtggggcgg ggaagtgggg cctggcttgt gcggcgtcgc
    18721 agacgttttg aaacaatctg ctgaaaacga accctgaggt cagctcggaa gcggctctgg
    18781 acagtgggcg gctgccaggg ccagcagctc ggcgttttcg gtcagcttgt acaggaagtc
    18841 gtttcgcgcc tcgcttctgg tgatggctgc cgcttggcgc agaagccgcg ttcgtccttc
    18901 cgcgagcagc gctttggcgg tctcgttttg cccgatcgcc agcttgatgt tcgccacggt
    18961 gtgatagacc agcccttcat ctgtgccagc gcgtcccagg acttgtagct gtgccaagct
    19021 tgcttccgct gccagtgccg cggcgtcgat gttcccgcgc tgctgttcaa tccgggccag
    19081 ccatgcaagg gcgcgcacgc gaaa
//
LOCUS       DAORGN010000083        18791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1176_length_18791_cov_5.672769, whole genome shotgun sequence.
ACCESSION   DAORGN010000083 DAORGN010000000
VERSION     DAORGN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18791
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1176_length_18791_cov_5.672769"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKL17_10880"
     CDS             complement(<1..1192)
                     /locus_tag="PKL17_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45280.1"
                     /translation="MTRVDGPQALVKLLPMVGRLRATHTLLGENNTAHLALVSPEPDP
                     ADDPVIGTVSVQSLVPPYPLSELPERVVAFPPAPARGFCTAQLGGAFDFPQLFLHRPD
                     RPADGPFTALVQPADKIRLLCGNSSLLAYGSSRDNLGMDVLRRKPDGTVVHKVLPWPS
                     QAEPAWGQGPLGFDQDERVVFILGGDIVTRAYYLDSGQNVDLGILYWAAPSHRGELFV
                     DVDGMLLLYGVDTQRSTYLGVRVSPDGAIVGIDQAHEAVLTCDWDGLRRIPLRPPFAL
                     DSQNVQVLDREPCRAIDWDGSPQASVLYYVGSQLRQVATDGKSPPVIVTNYQGQQMFA
                     LCDDGTLAYSHDPPERYGRGVGDGYIQGRRFMQRGRDVRFSPDCRHVYFKENAATLRR
                     LGQLR"
     gene            1356..1856
                     /locus_tag="PKL17_10885"
     CDS             1356..1856
                     /locus_tag="PKL17_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNK45281.1"
                     /translation="MKPNFFLVKSEPYKYAFSKLVSQGRTTWDGVRNFEARNNLRSMR
                     EGDLLLFYHSNEGKEVVGVATVVKTAYPDPTAPGEDWSVVDVAPVCALRSSVTLSMIR
                     SEPRLANIALLRRSRLSVVPVTADEFSVVLELGQTDVPGISPAVRSAKKTARRKTPSQ
                     EAEGRP"
     gene            1853..4393
                     /locus_tag="PKL17_10890"
     CDS             1853..4393
                     /locus_tag="PKL17_10890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNK45282.1"
                     /translation="MNHTSAQTPSGLNEREAKARLARFGKNELSGQEGPSFWRTLLSV
                     LTEPMLLLLGACGGLYLLLGDRGEAGLLFSFVGLVVAITLWQERRTERAVAALRSMQS
                     PRAQVVRDGVSQSIAASEVVPGDLLVLSEGVRVAADAILRSGQNVVCDESLLTGESVP
                     VRKVSVQGEAASARPGGDDLPQLYMGTMVVRGEGVAEVVATGLSTEVGKIGKALAEIE
                     PPKTPLDLEMKRLVRGMATLGVAMCVLVVLLRGLLYRQWVQGLLAGLAMAMSVLPEEL
                     PMILTVFLSLGAWRIAHSNVLLRRPGVIEALGAATVLCTDKTGTLTQNRMTVARLCCG
                     GVDHLVTGLPTPLPGDVERLLRAAKLASPTAPNDPMEQAIVALSAERRIVADGSGPCL
                     REYPLSPTLLAVTRAYEQGSGHVLFCKGAPEALATLCALSADEHAALSKQVEALAQAG
                     LRVLAVGQADVGAQLPDVPSAVRFSLLGLLAFLDPLRPEVPQAVADCRRAGVRVLMIT
                     GDYPATALAIAGQAGLDVTHGVLSGPEMAALSDESLRERVAHVSVFARMVPEQKLRLV
                     KALKDLGAIAAMTGDGVNDAPALKAAHIGISMGKRGTDVAREASALVLADDNFASIVA
                     AMRLGRRIFDNLRKAVAYTFAIHVPIAGLSLLSPLLGWPLILLPSHIAVLEMIIDPSC
                     GLIFEGSAEEAHIMDRPPRPVGQSMVTGRVLLLSIVQGFLVLLGCAFAYYLAAQRGYP
                     AERLRAMVFSTLVFANLGLIVVSRSWTESALRTLWKGGAVLWTLILGTMGLGLLVLQV
                     PFLQRLFSLSALPLSDALWALSLATLSVIWFEPVKLIWRRRLREMTVT"
     gene            4433..5839
                     /locus_tag="PKL17_10895"
     CDS             4433..5839
                     /locus_tag="PKL17_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK45283.1"
                     /translation="MLNQIQEFVALLRKNGVPASTAELLDAVSALSLVGMDDPQLVRA
                     VLKTCLVKRAEDESRFDDLFGLFFFRAADVLSSSPDAPLAVALAQAGFSEEQIERAMA
                     ALATEAARLDPTARLGLGLRRVGLDLLLRLSGLRIDVSRMQSSLQIGYFTQGLIDQLG
                     FRQAESELRGLMNRLSPELGPELAQTVLRQAEQNLWQLRGALRGEVQRAFEKQNLRFA
                     ETQRSELLSQKPLSQLSAHELALLRQQVVRLADKLKTQQRDKPQRQQKGRLDMRRTLR
                     AALSSGGIPFRLRFRTKHPHKPRLVILCDISDSVRQVARFMLQLAYTLQERFSKVRSF
                     VFVSDLGDATELFESNEIERAVEKTYSGAIVSVASNSNYGRALRQFVEKHLDAVTSRT
                     TVIVIGDGRSNYFPSEAWALARVKARARHVLWLNPEQPSSWSFGDSAMRDYLPHVSRA
                     ETVTNLQSLRRVIDELLL"
     gene            5960..7192
                     /locus_tag="PKL17_10900"
     CDS             5960..7192
                     /locus_tag="PKL17_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45284.1"
                     /translation="MPQATDHVAHRCVSSRSFAKQRLFATLFLALAPLLFASSDGGAQ
                     VRAGKAEAPAIVAVAPMAVQAAVVPQATAATPGSTGTPASGTAQVVVASGSSPAITPV
                     SPAWTDTLNKIVPDPPPPHLVRGTHYVISNEDRHDLWLKTVQGKGGAYLGVGTDQNYV
                     LAGWQKPELMVLFDFDQVVVNLHYVYRVFFISAGTIDEFKSLWEVKNEATAMKLLTDA
                     YPHPKQRAAVVEAYKMSRGSVLARFRKVLGIYKVHNIRCFLDDQSQYDHLAQLFRTGR
                     VVMVRGDLTVGGSLSSIASALQGIGVKLSVLYLSNAERYWTYTDGFRKSITALPFGDD
                     ATVIRTRARPNGVYMYVVQDAKNFLEWVRHPKVNTVYQVTHSMDPDPNPGLYYIRKSP
                     AIAVPSVPHPPFAHLKAN"
     gene            7324..7677
                     /locus_tag="PKL17_10905"
     CDS             7324..7677
                     /locus_tag="PKL17_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HNK45285.1"
                     /translation="MGFVSPLKVLGVLVVGSMLLAPLASANFKNLQVLPKNVTKGALI
                     AQMNAQVVAIGKDCTHCHDPKDPSAETPMKEKTRDMMRMVTELNTKWPGTMNRITCWT
                     CHRGNHKPQDKPAAP"
     gene            7897..9474
                     /locus_tag="PKL17_10910"
     CDS             7897..9474
                     /locus_tag="PKL17_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45286.1"
                     /translation="MRWVALLCSLLACVYYLAFQSHRLGFDALDYAYGVKRGHNLFHP
                     HHLLYNAVGRALYVAVSALHPVDALSLLASQNAVVTAASVGLLCLLLFRVTQRLSVSI
                     GLCLLFAGLRGVTAMATSVEVYPATTLFELCAISLWLLRPVPSLGTLAAMGICCGLCA
                     LFHQTGIFFVLAMAYGLWTQDRRPSRVLCFLAAAFAVCGGAYLYVGMVVEKRPLGGLW
                     TWVTAYAHSDEYRLGMWGHGLRLQSLPSAAQGLFQVITAPYYLENLSRQFRPSVWDVV
                     LSLSLLASLGLWLSLLVTALRRPKGIASHGAEDLANTHLAAQTLRPLLMLWIGLHSAF
                     TLWWEPGNFEFWLLAAPPTVLLCGSLCRRPPNAKKQWQMSVALGLLFFGNFGLCTLPS
                     HRGVDNPNAEIYRILSTQTVDSEDIYVGDFATLQLYFLYYADKRVPLHSLRFEGYSRP
                     SQKAEVIDSYRARIDNIRRQHRVFFLEHERFDQYDAFDREDVERMYAPMLIGATEIGS
                     YQKGADTYRILRLAKLP"
     gene            complement(9471..10949)
                     /locus_tag="PKL17_10915"
     CDS             complement(9471..10949)
                     /locus_tag="PKL17_10915"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK45287.1"
                     /translation="MQTRIVICGSGFAGLGMAIRLKQAGIHDFIVLERASEVGGTWRD
                     NHYPGAACDIESHLYSFSFEPNPRWSRQYAPQKEILDYLVHCADKYNIRPHIRFGSTV
                     QSARYDEQSARWEVSVSDGENISCQFLITACGGLSKPATPDIAGLSSYAGKMFHSARW
                     DHSVSLSGKRVAVIGTGASAIQIVPSIAPQVAQLAVFQRTPPWIMPKRDHAISPSAQD
                     RFARHPLVQKLARTLIYITHELAGFGFVVEPKLLKLLSRLVLRYLKRSIPQEELREKL
                     TPNYAMGCKRILISNDYYPALRRQNVELVTSPIDCVNTSGIRTQDGQQRDFDAIVLAT
                     GFDAADIVCPFPMCGRSGIQLSDVWRDGAEAYLGTTVSGFPNAFLIVGPNTGLGHNSM
                     IYMIESQIAYILSAIEQMSRDGIASVEVKQEVQTRFNQEIHKRLLGTVWSSGCASWYQ
                     TKNGKNTTLWPGFTIEYRARTRRFSRRDYIVKTASAGIPSPA"
     gene            complement(10963..11796)
                     /locus_tag="PKL17_10920"
     CDS             complement(10963..11796)
                     /locus_tag="PKL17_10920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNK45288.1"
                     /translation="MQAAALPVDKSVPTPRSPELPLSGPIPRHWFAGNVLATHMINGV
                     SMLFPAGERFFVRSVNHYLDRISDPHLRAQVKGFFGQEGRHAKEHDRWNDILQSQGYS
                     VKSYLAFYEWFGYHVIERLATPGLRLAATAALEHFTALLAEKALRYHALDHADPTMRA
                     LLLWHAAEEIEHRSVAFDVLAQMEPSYALRMAGLVLATATLSGFWIGAATMLLWQDRK
                     SLFASLRKDLQNIAAVRDRNKDRGLFLPGILSYLRRDFHPSQVDLDKLAQDYLTAAQL
                     A"
     gene            complement(12018..12833)
                     /locus_tag="PKL17_10925"
     CDS             complement(12018..12833)
                     /locus_tag="PKL17_10925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNK45289.1"
                     /translation="MNASFVLHCLDAALASAHAAGKVLAEGAFRRHAAESKSATTDLV
                     TRYDRASEEIVCAELRRRFPSHRILAEESGDHGGRSDEPRWIIDPLDGTTNFAHGLPL
                     FSISIACEWEGQVHVGVVHAPALGWTFSAARGHGAYRNGERLSVSPCDAIERALLVTG
                     FPYDRKTARENNFAQFFALKRRAQGIRRLGSAALDLSLVASGSLDGYWEMKLKPWDIA
                     AGLLIAQEAGATTTDWQGQPVSLKLGEVLCAGPTLHRELLAVLRTVEHGQFSV"
     gene            complement(12881..15658)
                     /locus_tag="PKL17_10930"
     CDS             complement(12881..15658)
                     /locus_tag="PKL17_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45290.1"
                     /translation="MTSPADPKAVQAMPAWVAGNPVPPEVRLGKVLQNEFHILRVLGE
                     GELGVTYEAENSRLKGKFAVLMLRRELRPTQGMMLAVQKDLRAAQPLQAMGLLPVKML
                     VDQFDIPGFATELLDGETLRQRLSRGPLSIERALALILYLGKALDALHKAGAVHGDLR
                     PENIFLVRPGAKSTMAGKPVIVEHSLHHLRRRSVGLDDQLPLYKLMYRPPELLAGETG
                     PHPGGDVFVLGAILHECLTGRPAFFDELADFVIDNLHQPPKKLEPNPNTGLTAQLCQS
                     LDELTECACARAPKERLPDMGSLVAGLEALVKTAGLRLPDVLTEVPGTVDPVASQAQK
                     RMHQLLERRSGVFPVLGPPPSQPVTKPAEPAPVAMAEPAKPTPAPASPTPAKTAEPLP
                     QLQAITAPKKKVTQILQQLSTAFPVLTVSPDSGSIEEQIVKPQAKIDDTPAPETKPEP
                     KPETSEAKGAEPSVLAKPEPAVEPKPAQTTVPATVAEEPKPSEKPVATQQAEPTKAEA
                     STSTTEATTQPKAPDARPVLKAGVAEALARLRKPAAPVELKGPPLVAELPFAVPATPQ
                     VQSVPATPPVQSVPATPQVQSVVEPGKTAPPIAPEPKPAAEAKPSVEPKPAEPKPVAE
                     FKAPSPKRVSESTLQVDNVEDLLVSLPSLAAIPIVPPSPPPPPVSPVEPQTLSSVPTS
                     AAIEKLEAQHDPALLSAPTKPLLPAMIGELLTPPVNPTPPPSLRLEPQVTPAVTAPPN
                     AATLHEQPTRTKLPVDAISMLPTQAAVEAMPPQPIVQAPADTPSSGKAPASANDGLHE
                     KPTAASIPSLDLLSRLQEKAGSGPARDAQKLLESNPIVRPEPAKEPTPPAAKPAEPVA
                     DAKPVLPAQVSTSHASFAPRRPSSKPVALPGSPEPGLRSLLLKHQEVVAASVGALLVL
                     LIGLLFYAIIR"
     gene            15787..17322
                     /locus_tag="PKL17_10935"
     CDS             15787..17322
                     /locus_tag="PKL17_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNK45291.1"
                     /translation="MTAVSIGSFPLSQRSLRLPLFFSVLAVARSAAAGGFEIPANGTE
                     ALGRGGAFSAKADSPLALEYNVAGLAQQRGTHALIDNNLAFSRYRFARDGGDSFGPFP
                     AVQANATPPFYAPWFGLATDFGFFKRLTFAVGIYGPSSIGKRTFPMFVPMDSGKDRPG
                     PARYDIATTDLLIFFPTLAVGVRASKYLDLGFSVQQASAQIKLASMTYAPQSLPIFPQ
                     SSACSMQAEVAGCDSLTRITGSSYDNYLLQLGALAHLGKSVDLGFNVRSASNLGIRPI
                     VVRGTVSATEPLYLQGAGIGAEKMDGEFTINLPWVFRGGVRYGQKLGGTQLFDVELDA
                     IYEAWSWLDGTDHQLTLKNPPPLVNKGQPVQITLPHHYRDTIGLRLGGSLYMPLAADA
                     GVTMRMGGFYDSSASNDADLRLDFDTLEKLGICAGLGFSLRGVEVNFAYSYQHSLPRT
                     VSNGQLSVIDGTTGQPVQVGGMSTPAINNGTYSGSTHIASIGLTLRFDEWRPSYKSSS
                     EGL"
     gene            complement(17308..17862)
                     /locus_tag="PKL17_10940"
     CDS             complement(17308..17862)
                     /locus_tag="PKL17_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNK45292.1"
                     /translation="MRTLAQVLALLLSLQTAQVHAQTPVPEELLLRRQEAQLLHRRMD
                     LSTLRVLRKNEFVATEAPVARLLFVHLWAVECRPCLEELPTLRSFFEAQAKDPRLKLV
                     IASETRDVPKLQEFLKQHRGYFPNGDLYQVIDDRLRSSLQNFAQPLTLLVDERGVVHH
                     AFVGSLRSRRSELVDAVERYLKSL"
     gene            complement(17881..18681)
                     /locus_tag="PKL17_10945"
     CDS             complement(17881..18681)
                     /locus_tag="PKL17_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45293.1"
                     /translation="MSGRRWRVLLGAAMVFSASCYAEEIADLRGQVERMQRCQVCGQR
                     QLQKGLALALCRPPVRQLIDDVAQVCKREDICLDQKIATLVGDADPTHLGRFVAMMSG
                     QTHSVLYFTPKDTLIDGLEGVYLQRLKRTIQPQPPWLETTKFLVVSNLPPPPLGQTGT
                     SKLSTQDPQVARAERRGLQIIEKLLDIQFPGGQKLKRDQVLHWVYSFSLKKREYLNPD
                     DMPPVGSELAQSVWVFRIDCATPTEQSEPTGCDDCLPPPALRPLESPK"
BASE COUNT         3346 a         5329 c         6200 g         3916 t
ORIGIN      
        1 tgcgtagctg gccgagccgg cgcagggtgg cggcgttttc cttgaagtag acgtggcggc
       61 agtcggggga aaagcggacg tcgcggcctc gctgcatgaa tcggcggcct tggatgtagc
      121 cgtcgccgac gccgcgtccg tagcgttcgg gcgggtcgtg ggaataggcc agcgtaccgt
      181 cgtcgcacag ggcgaacatc tgctggcctt ggtagttggt gacgatgacg ggtggggatt
      241 tgccgtcggt ggcgacctgg cgaagctgcg atccgacgta gtacagcacg ctggcttgcg
      301 gcgatccgtc ccagtcgatg gcgcggcagg gctcgcggtc aaggacttgg acgttttggg
      361 aatcgagcgc aaagggggga cgcagcggga tgcggcgcag gccgtcccag tcgcaggtca
      421 gcacggcttc gtgggcctgg tcgatgccga cgatggcgcc gtcgggggat acgcgaacac
      481 ccaggtaggt cgaacgctgg gtatctacgc cgtagagcag gagcatgccg tcgacatcaa
      541 cgaacagctc gccgcggtgg gatggggcgg cccagtacaa gatgcccagg tcgacgttct
      601 gaccgctgtc gaggtagtac gcgcgggtga cgatgtcgcc gccgaggatg aagacgacgc
      661 gctcgtcttg atcgaagccg agcggacctt ggccccaggc gggctcggct tggcttggcc
      721 agggcaggac tttgtgaacg acggtgccgt cgggtttgcg gcgcagcaca tccatgccga
      781 ggttgtcgcg gctcgatcca taggcgagca gggagctgtt gccacagagc agccggattt
      841 tgtcggcagg ctggaccagc gcggtgaagg gtccgtcggc gggtctatcg gggcggtgca
      901 aaaagagctg cggaaagtca aaggcgccgc caagctgggc ggtacaaaag ccgcgggccg
      961 gggccggcgg gaacgcgacg acgcgctcgg gcagctcgga cagtggatag ggcggcacca
     1021 gcgactggac cgaaaccgtg ccaatcaccg gatcgtcggc aggatcgggc tctgggctga
     1081 ccagcgccag gtgggcggtg ttgttttcac cgagcagcgt gtgcgtcgcc cgcagtcgcc
     1141 cgaccattgg cagcagcttg accaacgctt gcggcccgtc tacccgtgtc accgccagca
     1201 gctcttcgcc cgaaagctcc gagcaggcgc cgactagacc cagccatagc agcaagccat
     1261 aagctgtgaa tttccccaca gaaattgtcc cccgcgccca ttgtaaacgg cttttcccaa
     1321 accgggaatg ggggcgggtt tagtatcggg cggcgatgaa accgaacttc tttttggtca
     1381 agagcgagcc gtacaaatac gctttttcga agctggtcag ccagggacgg acgacctggg
     1441 atggcgtgcg caactttgaa gcccgcaaca acctgcggag catgcgggaa ggcgacctgc
     1501 tgctgtttta tcactcgaat gaaggcaaag aagtcgtcgg tgtcgccaca gtggtaaaga
     1561 ccgcgtatcc cgatcccact gcgccggggg aagactggtc ggtggtcgac gtcgccccgg
     1621 tctgtgcgct acgttcttct gtgacccttt cgatgattcg cagcgaaccg cgtcttgcca
     1681 acatcgcgct gcttcgtcgc agccgcttgt cggtggtgcc ggtgacagca gacgaatttt
     1741 ccgtcgtgct ggagctgggc cagaccgatg tgccggggat atcgcccgcc gtgcgcagcg
     1801 ccaaaaaaac ggctcgtcgc aaaactccca gtcaagaggc agaaggccgt ccatgaacca
     1861 cacgagcgcc caaacgcctt ccggtctgaa tgaacgcgaa gccaaggcgc ggcttgcccg
     1921 ctttggcaaa aacgagctgt cggggcaaga agggccgtcg ttttggcgca cgctgctttc
     1981 cgtcctgacg gagccgatgc tgcttctgct cggggcgtgc ggtgggctgt atctgctttt
     2041 gggcgaccgc ggcgaagcgg ggcttttgtt ttcgtttgtc gggttggtcg tcgcgattac
     2101 gctctggcaa gagcggcgta ccgaacgagc ggtggcggcg ctgcgcagca tgcaaagtcc
     2161 gcgtgcccag gtggtgcgcg acggtgtttc gcagagcatt gccgccagcg aggtggtgcc
     2221 tggggatttg ctggtgctgt ccgaaggcgt gcgggtggcg gcggacgcga tcttgcgcag
     2281 cggtcagaac gtggtctgtg atgaatcgct gctgacgggc gagagcgtgc cggtgcgcaa
     2341 agtcagcgtg caaggtgaag cggcgtcggc tcgtcctggc ggtgacgatc tgccgcagct
     2401 ttacatgggg acgatggtgg tgcggggcga aggcgtggcc gaagtggtgg cgacggggct
     2461 ttcgaccgag gtgggcaaga tcggcaaagc gctggcggaa atcgagccgc ccaagacgcc
     2521 gctcgatctg gaaatgaagc ggctggtgcg cggcatggcc acgctgggcg tcgcgatgtg
     2581 cgtgctggtg gtgctgctgc gcggtctttt gtatcggcag tgggtgcagg ggcttttggc
     2641 gggcctggcg atggcgatgt cggtgctgcc ggaagaactg ccgatgatcc tgacggtgtt
     2701 tctgtcgctg ggggcgtggc gaatcgcgca cagcaacgtg cttttgcggc ggcctggggt
     2761 gatagaagcg ctgggggcgg ccaccgtgct gtgcaccgac aagactggga cgctgaccca
     2821 gaaccggatg acggtggcca ggctgtgctg cggcggggtg gatcatcttg tgacgggctt
     2881 gccgaccccg ctgccggggg atgtggagag gctcttgcgg gcggcgaagc tggcgagtcc
     2941 gacggcgcca aacgacccga tggaacaggc gatcgtcgcg ctaagcgctg agcgccggat
     3001 tgtggctgac ggaagcgggc cttgtctgcg cgaatatccg ctttcgccga cgctgctcgc
     3061 ggtgacgcgc gcctatgaac agggcagtgg gcatgtcctt ttttgcaaag gcgcgccgga
     3121 agcactggcc acgctgtgtg cgctgtctgc cgatgaacac gcggcgcttt cgaaacaagt
     3181 cgaggcgctg gcgcaagcgg gcctgcgggt gctggcggtt gggcaggccg atgtcggcgc
     3241 acagcttccc gatgttccgt ccgcggttcg tttttcgctg ctgggacttt tggcgttttt
     3301 ggacccgctg cgtcccgaag tgccgcaagc ggtggccgac tgccggcgcg ccggggtgcg
     3361 ggtgctgatg atcacggggg actatccggc gacggcgctg gcgattgccg ggcaggcggg
     3421 tcttgatgtg acgcacggtg ttttgtcggg gcccgagatg gctgcactgt ccgacgagtc
     3481 gctgcgcgag cgagtggccc atgtgtcggt gtttgcgcgg atggtgcccg agcaaaagct
     3541 gcggctggtc aaagcactca aagacctggg cgcgattgcg gcgatgactg gcgatggggt
     3601 aaacgatgcg ccggcgctga aagcggcgca catcgggatt tcgatgggca agcggggcac
     3661 cgacgtcgcg cgtgaggcat cggcgctggt cttggccgac gacaacttcg cttcgatcgt
     3721 ggcggcgatg cggcttgggc gacggatctt cgacaacttg cgcaaggcgg tggcgtatac
     3781 gttcgcgatc catgtgccga tcgccggact gtcgctcttg tcgccgcttt tgggctggcc
     3841 gctgattctt ttgccgtcgc acatcgcggt gctcgaaatg atcatcgatc cctcgtgtgg
     3901 gctgattttt gaaggcagcg ccgaagaagc ccacatcatg gatcgtccgc cgcgtccggt
     3961 cggccaatcg atggtgacgg ggcgggtgct gcttttgtcg atcgtgcagg ggtttttggt
     4021 gctgcttggc tgcgcctttg cgtattacct ggctgcgcag cgaggctacc cggcagagcg
     4081 actgcgggcg atggtgtttt cgacgctggt gttcgccaac ctggggctta tcgtggtcag
     4141 tcgatcgtgg acggaatcgg cgctgcggac gctgtggaaa ggcggggcgg tgctgtggac
     4201 gctgattttg gggacgatgg ggcttgggct gctggtgctg caagtgccgt ttttgcagcg
     4261 gctgttttcg ctgtcggcac tgccgctatc agatgcgctg tgggcgcttt ctctggcgac
     4321 gctgagcgtg atttggtttg aaccggtgaa gctgatttgg cgacggcggc tgcgggaaat
     4381 gacggtgacg tagcggcgtc ttgggcggcc aagggaaaaa gcagagcagg cgatgctgaa
     4441 ccaaatccaa gagtttgtgg cgctgttgcg caaaaatggc gtgccggcct cgacggcaga
     4501 gctgcttgat gcagtgtcgg cgctgtcgct tgtggggatg gacgatccgc agcttgtgcg
     4561 ggcggtgtta aagacgtgtc ttgtgaagcg ggccgaggac gagagccgct ttgacgatct
     4621 gttcgggctg ttttttttcc gcgcggcgga cgtgctttcg tcgtcgccgg atgcgccgct
     4681 ggcggtggct ttggcccaag cgggtttttc ggaggaacaa atcgagcgag cgatggcggc
     4741 actggcgacg gaagcagcgc ggcttgatcc gacggcacgg cttgggcttg ggctgcgacg
     4801 ggtggggctt gatttgctgc tgcggctgtc ggggctgcgg atcgatgtgt cgcggatgca
     4861 aagctcgctg caaatcggct atttcaccca agggcttatc gatcagcttg ggtttcgcca
     4921 ggccgagtcg gagctgcgcg ggctgatgaa ccggctgtcg cccgagcttg ggcccgagct
     4981 tgcgcagacg gtgcttcggc aggcggaaca gaacctgtgg cagctccgcg gcgccctgcg
     5041 tggcgaagtc cagcgtgcgt ttgaaaagca gaacctgcgc tttgccgaga cgcagcgcag
     5101 tgagctgttg tcgcaaaaac cactgtcgca gctatcagca cacgagctgg cgcttcttcg
     5161 gcagcaagtg gtgcggctgg ccgacaagct gaaaacccaa cagcgcgaca aaccgcagcg
     5221 gcagcagaag ggtcggctcg acatgcggcg gacgctgcga gcggcgctgt cgtcgggggg
     5281 aattccgttt cggctgcgtt ttcggaccaa acatccgcac aagccgcggc tagtcatttt
     5341 gtgtgacatc tcggattcgg tccgtcaggt cgcgcgcttt atgctgcagc ttgcgtacac
     5401 gctgcaagag cggttttcga aggtgcgatc atttgtgttt gtgtcggatc tgggcgacgc
     5461 gaccgagctg tttgaaagca acgaaatcga gcgtgccgtc gagaaaacct attccggggc
     5521 catcgtcagc gtggcgtcga actcgaacta cggacgggcg ctgcggcagt ttgtcgaaaa
     5581 acacctggac gccgtgacct cgcggacgac ggtgattgtg attggggacg ggcgatcgaa
     5641 ctactttccg tccgaagcct gggcgctggc ccgggtcaaa gcgcgggcgc ggcacgtcct
     5701 gtggctcaat cccgagcagc cgtcgtcgtg gtcattcggc gacagcgcaa tgcgcgacta
     5761 tctgccccac gtcagccggg ccgaaacggt cacgaaccta cagtcgctcc ggcgcgtgat
     5821 tgatgagctt ctgctgtagg tctggccggc aaatgatgac ataaaaacgt gtaagcccgt
     5881 ttacttgcgg gtttttttgg cggtatgatc gggccggggg atcctgtctc gcttgagcga
     5941 gtggcccccg aggctcccaa tgccccaagc cacagaccac gttgcgcatc gttgtgtttc
     6001 gtcccggtcg tttgccaagc aacggctttt tgccacgctg tttttggcac ttgcaccact
     6061 cctctttgca agctcagacg gtggtgcgca ggttcgtgcc ggcaaggcag aagctcctgc
     6121 gatcgtcgcg gtggcaccca tggccgtgca ggcggcggtg gtcccacagg cgaccgcagc
     6181 gacgcccggc agcacgggca cgccagcttc ggggaccgcg caggtggtgg ttgcttccgg
     6241 cagctcgcct gccattacac cggtcagccc ggcgtggacc gatacgctca acaagatcgt
     6301 gcccgatccg ccgccgccgc acctggtccg cggcacgcac tatgtgatct caaatgaaga
     6361 tcgccacgat ctgtggctaa agacggtgca gggcaaaggc ggcgcgtact tgggcgtcgg
     6421 caccgaccaa aactacgtgc tggccggctg gcaaaaaccg gaactgatgg tgctgtttga
     6481 cttcgatcaa gtcgtcgtca acctgcacta cgtctatcgg gtgtttttca tcagcgccgg
     6541 caccatcgac gagtttaagt cgctgtggga agtgaaaaac gaagcgacgg cgatgaagct
     6601 tcttaccgac gcctatccgc atcccaaaca gcgtgcggcg gttgtcgaag cctacaagat
     6661 gtcgcgcgga tcggtgctgg cacgctttcg caaagtgctg ggcatctaca aagtgcacaa
     6721 cattcgctgc ttcctcgacg accaaagcca gtacgatcac ttggcgcagc tgttccggac
     6781 cgggcgcgtc gtgatggttc gcggcgattt gaccgtcggt ggaagcctgt cgagcattgc
     6841 gtccgcgctg caaggcatcg gcgtcaagct cagcgtgctg tatctgtcga acgccgagcg
     6901 gtactggacg tacaccgatg gtttccgcaa gagcatcacc gcgctgccgt ttggcgacga
     6961 tgcgacggtg ataaggacgc gggcccgacc gaacggcgtc tacatgtatg tggtgcagga
     7021 tgccaaaaac ttcctcgagt gggtcaggca tccgaaagtg aacacggtct atcaagtgac
     7081 ccacagcatg gaccccgatc ccaatccggg gctgtattac atccggaaaa gtcccgcgat
     7141 cgcggtgccc agcgtgccgc acccaccgtt tgcccatctc aaagccaact agccaaacgc
     7201 agtggttgtc gagggtcgcg cgctcgtcgt agcatctttt gcgaataaag cgcgtggtcc
     7261 cgccatcgtt acggatggcc aacgtgcacg gcctctggtt cgcaactgag gagtcagcga
     7321 tcgatgggtt ttgtctctcc gctcaaagtt ctgggtgttc ttgtggtcgg ttcgatgctg
     7381 cttgccccgc tggcgagcgc caacttcaag aaccttcagg tattgcccaa gaacgtgacc
     7441 aagggagcgc tgatcgcgca gatgaatgcg caggtggtgg cgatcggcaa agactgtacg
     7501 cactgccacg atccgaaaga tccgtcggca gaaacgccga tgaaagaaaa aacgcgcgac
     7561 atgatgcgga tggtcacgga gctcaacacc aagtggccgg ggacgatgaa tcgcatcacc
     7621 tgctggacgt gccaccgcgg caatcacaaa ccacaagaca agcccgcggc tccctaacat
     7681 tcactgcccg tacattgcgt tggttctggc gcggacgctc gcttgggaat ccggcggact
     7741 gaccgtttgg attcccaaca aactgcgcta gcatccgcgc tgccgatggc gctttcttcc
     7801 cgaaactctg ccccgcgaaa gtcgacgacc gatccggcgt ttcatgacgc tccggctgtt
     7861 tcgccgccgg tcccattgcg ttctagtgcg tcgcgtgtgc ggtgggtcgc gcttctgtgt
     7921 tcgctgctgg cctgcgtcta ctatctggcg tttcagagtc accgcttggg atttgacgcg
     7981 ctcgactatg cgtatggtgt aaagcgcggt cacaacctgt ttcatcccca tcacttgctc
     8041 tacaacgccg tcggacgtgc gctgtatgtc gcggtgtctg cgcttcatcc ggtggacgcg
     8101 ctttcacttt tggcttcgca aaatgcggtg gtgacggcgg cttcggtggg gcttctgtgt
     8161 ctcctgctgt ttcgggtgac gcagcggctg tcggtctcta tcggactgtg tcttttgttt
     8221 gcgggtttgc gcggtgtgac cgcgatggcg acatcggttg aagtctatcc agcgacgacg
     8281 ctgtttgagc tgtgcgcgat cagtctgtgg cttttgcggc ctgtgccttc gcttgggacg
     8341 ctggctgcga tgggaatctg ttgcggactt tgcgcgctgt ttcaccagac cggaatcttt
     8401 tttgtcctgg cgatggccta cggtctgtgg acacaagatc gcagaccaag ccgcgttctg
     8461 tgtttccttg cggcggcatt tgccgtctgt gggggcgcat atctgtacgt tgggatggtg
     8521 gtcgaaaaac gaccgcttgg gggtctgtgg acgtgggtga ctgcgtatgc gcattccgat
     8581 gaatatcggt tgggaatgtg gggacacgga cttcgcttgc agagtctgcc ttcggctgcg
     8641 cagggactgt ttcaggtcat caccgcgccg tattacctgg aaaacctaag tcggcagttc
     8701 cgtccgtctg tctgggatgt ggtcctgagc ctgtcgctgc tggcgtcgtt gggactgtgg
     8761 ctttcgctgc ttgtcactgc actgcgccgc ccaaaaggaa tcgcttcgca cggggcggag
     8821 gatttggcaa acacgcactt agctgcgcaa acactgcggc ctctgctgat gctgtggatc
     8881 ggtctgcact cggcttttac gctgtggtgg gaaccaggga attttgaatt ttggctgctt
     8941 gctgcaccgc cgaccgtctt gctgtgtggg tcgctgtgcc ggcggccccc gaacgcaaaa
     9001 aaacagtggc agatgtccgt cgcgctgggg ctcttgtttt tcggaaactt tggtctgtgt
     9061 acgctgcctt cccatcgcgg tgtcgacaat cccaacgcag agatttatcg cattctgtcc
     9121 acccagaccg tcgattccga agacatctac gtgggggatt ttgcgaccct tcagctgtat
     9181 tttctctact acgcagacaa gcgggttccg ctgcattccc tgcgctttga gggatacagc
     9241 cgtccgtcgc agaaagccga agtgatcgat tcctatcgcg cgcggatcga caacattcgc
     9301 aggcagcatc gcgtgttctt tttggaacat gagcgcttcg atcagtatga cgctttcgat
     9361 cgggaagacg tggagcgaat gtatgcaccg atgttgatcg gtgcgacgga aatcggcagc
     9421 tatcaaaaag gcgccgatac ctatcggatc ttgcgacttg caaagcttcc ctaagccgga
     9481 ctggggattc cagcgctcgc ggtttttacg atgtagtctc gacgggaaaa tcggcgggtg
     9541 cgggctcggt attcgatggt gaagcccggc cacagcgtgg tgttcttgcc gtttttggtt
     9601 tgataccaac ttgcgcagcc gctggaccac acggtgccga ggagccgctt gtgaatttcc
     9661 tgattgaagc gggtctggac ttcttgtttg acttcgacag atgcgattcc gtcgcggctc
     9721 atttgctcga tcgccgacag gatgtacgca atctgcgact cgatcatgta gatcatcgag
     9781 ttgtgtccaa ggccggtgtt gggtccaacg atgaggaatg cgtttgggaa tccgctgacc
     9841 gtggttccca aatacgcttc ggcgccgtcc cgccacacat cggaaagttg gattcccgat
     9901 cgaccgcaca tcgggaacgg acagacaatg tctgcggcat caaatcccgt cgccaagacg
     9961 atcgcatcaa agtcgcgctg ctgtccgtct tgggtgcgga ttccgcttgt gtttacgcag
    10021 tcgattgggc ttgtgacaag ctctacattt tgccgtcgca gcgctggata gtagtcgttg
    10081 gaaatcaaga tccgcttaca acccatcgcg tagtttggcg tcagtttttc gcgcagttct
    10141 tcttgcggaa tgctccgctt cagatagcgc aagaccaagc gtgaaagcag cttgaggagc
    10201 ttgggctcga cgacaaaacc gaatcccgcc agctcgtggg tgatgtagat caaagtccgc
    10261 gcgagctttt gaaccagcgg atgtcgggca aagcgatcct gcgcggacgg acttatcgca
    10321 tgatcgcgtt tgggcataat ccacgggggc gttcgctgga acaccgcaag ctgggccacc
    10381 tggggggcga tcgacggtac gatctgaatg gcactggctc cggttccgat caccgcgacg
    10441 cgctttccgg acagggaaac gctgtgatcc cagcgtgcag aatggaacat cttgccggca
    10501 taggaagaca gtccggcgat gtccggagtc gctggttttg acagacctcc gcaggcggtg
    10561 ataaggaact gacaagagat gttttcaccg tcggaaacgc tgacttccca gcgcgcactc
    10621 tgttcatcgt accgggcact ttgtaccgtg ctgccaaagc gaatgtgcgg acggatgttg
    10681 tacttgtctg cgcagtggac aagataatcc aggatctctt tttgcggcgc gtactgccga
    10741 gaccagcgcg gattgggctc gaacgaaaag gaatacagat gcgattcgat gtcacacgcc
    10801 gcgccgggat agtgattgtc gcgccaggtg ccgccgactt cgcttgcgcg ttcaaggaca
    10861 atgaagtcat ggatgccggc ttgtttgaga cggatggcca ttcccaaacc ggcaaagccg
    10921 ctgccacaga tcacaatccg ggtctgcatg gcggattcct tcctacgcga gctgcgccgc
    10981 ggtgaggtag tcttgggcga gcttgtccag atcgacctgg gaagggtgaa aatcccgtcg
    11041 cagatacgaa agaattccgg gcagaaacag accgcgatct ttgttcctgt cacgtaccgc
    11101 ggcaatgttt tgcaggtctt tgcgcagcga agcaaacaga ctcttgcgat cctgccacag
    11161 cagcatcgtt gccgcgccga tccagaaacc cgacagcgtc gcggtggcca gtacaagacc
    11221 cgccatccgc agcgcatagc ttggttccat ctgcgcaagg acatcaaatg caaccgatcg
    11281 gtgttcgatc tcctcggcag catgccaaag caaaagcgcg cgcatggtcg gatcggcatg
    11341 atcgagcgcg tgatagcgca gcgctttttc cgccaatagc gcagtaaaat gttcaagtgc
    11401 tgccgtcgct gcgagtcgca gtcctggggt cgccagccgt tcgatgacgt ggtatccgaa
    11461 ccactcatag aacgcgaggt acgactttac ggaatagccc tggctttgca ggatgtcatt
    11521 ccagcgatcg tgctctttgg cgtggcgtcc ttcctgtccg aaaaacccct tcacttgcgc
    11581 gcgcaggtgc gggtcgctga ttcgatccag gtagtgattc accgaacgga caaagaagcg
    11641 ctcaccagcg ggaaacagca tgctgacgcc gtttatcatg tgcgtcgcca gcacgttgcc
    11701 tgcaaaccag tgccgtggga tcggaccgga caagggaagc tcgggtgaac gtggcgtagg
    11761 aaccgacttg tcgacgggaa gtgctgctgc ctgcatcgct gttgactcct gttcaatagc
    11821 caatgttcaa acgatagcgc ttattgaacg aaagtcaata cgagggacaa aaagcggcaa
    11881 caaccgacgg aacatccgca gttcggcgaa aacacaggcg ggcaggaaat cgaccggaga
    11941 ggccggaacg gaaaaccaaa tgggaaggcg gaatgggaag gcggaatggg aaggcggaac
    12001 cccagaccag cagaaaacta gacggaaaac tggccgtgtt cgacggtgcg caagacggcc
    12061 agcagctcgc gatggagcgt cggtcctgcg cacagcactt cgcccagctt gagcgagacc
    12121 ggctgtcctt gccagtccgt cgtcgtcgct ccggcttctt gggcaatcaa gagacccgca
    12181 gcgatgtccc agggctttag cttcatttcc cagtatccat cgagactgcc ggaagcaacc
    12241 agcgacagat cgagcgccgc cgagccgagc cggcgaatcc cttgggccct tcgcttgagc
    12301 gcaaaaaact gggcgaagtt gttttcgcgc gctgtcttgc ggtcgtaagg aaaacccgtg
    12361 accagcagcg cgcgctcgat ggcatcacac ggcgacacgc tgagccgttc accgttgcga
    12421 taggcgccgt gaccgcgcgc cgccgaaaac gtccatccaa gcgctggcgc gtggacaaca
    12481 ccgacgtgaa cttggccttc ccactcacac gcgatcgaga tcgaaaacag tggcagaccg
    12541 tgcgcaaagt tggtggttcc gtcgagcgga tcgatgatcc agcgtggctc gtcgcttctg
    12601 ccgccgtggt cgccgctttc ttcggcgagg atgcggtggc tgggaaagcg acggcgcagc
    12661 tcggcgcaga cgatttcttc cgacgcgcga tcgtaacggg tgaccagatc ggtggtcgcg
    12721 gacttgcttt cagcagcatg gcgacgaaaa gcgccctcgg cgagcacttt tcccgccgcg
    12781 tgggccgacg cgagcgccgc atccagacag tgaaggacaa agcttgcatt catggcagaa
    12841 ggaatccccg cccaggtcgc tccggggcgc ggctcgcctt ctagcgaatg atggcgtaga
    12901 agagaagccc aatcagcagg acgagcagtg caccgacgga cgcggcgacc acttcctggt
    12961 gttttagcaa cagcgaacgc agtcctggct cgggcgaacc gggcagggcg actggcttgc
    13021 tgctcgggcg gcgcggggca aacgaagcgt ggctcgtcga cacttgcgcg ggcaaaaccg
    13081 gcttggcatc ggcgactggc tcggcgggct ttgctgctgg tggcgtcggt tctttggccg
    13141 gttcggggcg gacaatcgga ttgctttcga gcagcttctg cgcgtcgcgc gctggaccac
    13201 tgccggcctt ttcctggaga cggctcagca aatcgagact gggaatcgag gctgcggtgg
    13261 gtttttcatg cagtccatcg tttgcgctgg ccggtgcttt gccgctgctc ggcgtatcgg
    13321 caggggcttg cacgatcggc tgcggcggca tcgcctcgac ggcggcctgc gtcggcagca
    13381 tcgagattgc atcgaccggc agcttggtcc gcgtcggctg ttcgtgcagc gtcgccgcat
    13441 tcggtggcgc cgtgacggct ggtgtcacct gcggttccaa acgcagtgac ggcggcggcg
    13501 tcgggttgac cggcggcgtc agaagttcgc cgatcattgc cggcaaaagc ggcttggtcg
    13561 gcgcactgag cagcgccggg tcatgttggg cttcaagctt ttcaatcgcc gcggaagtcg
    13621 gcacagatga cagcgtctgc ggctcgacgg gggaaacggg cggcggcggc gggctcggcg
    13681 gcacaatcgg aatcgcagcc agcgacggca agctcaccag caagtcttct acattgtcga
    13741 cctgaagcgt cgattcggat acccgcttgg gcgacggtgc tttgaactcg gcgacgggct
    13801 tgggctcagc gggcttgggt tcgacgctgg gctttgcttc agcggcgggc ttgggttctg
    13861 gggcaatcgg tggggccgtt tttccgggtt cgacgacgga ctgaacctgc ggagtcgccg
    13921 gaaccgattg aaccggcgga gtcgccggaa cggactgaac ctgcggagtc gctgggacgg
    13981 caaatggcag ctcggcaacc agtggtggtc ctttgagctc gacgggggct gccggtttgc
    14041 ggagcctggc cagcgcttcg gcgacaccgg ctttgagcac gggcctggcg tccggcgctt
    14101 tgggctgcgt cgtcgcctcg gtggtgctgg tggacgcctc tgctttggtt ggctctgcct
    14161 gttgtgtcgc gacgggtttt tcggacggct tgggttcttc tgccaccgtc gctggcaccg
    14221 tcgtttgcgc aggcttgggt tcgacggcgg gttcgggttt ggccaaaacc gatggctctg
    14281 cacccttggc ttcgcttgtt tccggcttcg gttccggctt ggtttccggg gctggggtgt
    14341 cgtcgatttt cgcttgcggc ttgacgatct gttcttcgat cgaaccactg tcgggcgaaa
    14401 ccgtcaggac tggaaacgcc gtcgagagct gctgcagaat ctgcgtgact tttttcttgg
    14461 gtgccgtgat cgcttgcagc tgcggcagcg gctctgcggt tttggcggga gtcggactcg
    14521 cgggcgctgg cgtcggcttg gccggttctg ccatcgcgac gggggccggt tcggctggct
    14581 ttgtgacggg ctgactcggc ggcggcccca gcaccggaaa gacacccgag cgccgctcca
    14641 gaagctgatg catccgcttc tgcgcttggc tggccaccgg atcgaccgtt cctggcactt
    14701 ccgtcagaac gtcgggcagt cgaagtcccg cagtcttgac cagcgcttcg agcccagcga
    14761 cgagcgaacc catgtccggc agtcgttctt tgggagcccg cgcacaggca cactcggtca
    14821 gctcgtcgag cgactggcac agctgcgccg tcagcccagt gtttggattg ggttccagtt
    14881 ttttgggcgg ctggtgcagg ttgtcgatga caaagtccgc cagctcgtcg aaaaacgccg
    14941 ggcgacccgt caggcattca tgcaggattg cgcccaggac aaacacgtcg ccgccgggat
    15001 gcggtcccgt ctcccccgcg agcagctcgg ggggccgata catcagcttg tagagcggca
    15061 attggtcgtc gagaccgacc gacctgcggc gcagatgatg caggctgtgt tcgacgatga
    15121 cgggctttcc cgccatcgtg gattttgccc ctggccgcac caggaaaatg ttttcaggtc
    15181 gaaggtcgcc gtggaccgca ccggctttgt gcagcgcgtc gagcgctttg cccaaataga
    15241 ggatcagcgc cagcgcgcgt tcgatagaaa ggggcccgcg cgaaagcctt tgacgaagcg
    15301 tttctccgtc gagtagctcg gtcgcaaagc ccggaatgtc gaactgatcg acgagcattt
    15361 tgacgggcaa aagacccatc gcttgcagcg gctgggcggc gcgcagatcc ttttgcaccg
    15421 ccagcatcat gccttgggtt ggccgcagtt ctcgacggag catcaagacg gcaaacttgc
    15481 ctttgagccg tgagttttcg gcttcatacg tcacgcccag ctcgccttca ccgaggacac
    15541 gcaggatgtg aaattcgttc tggagaactt tccccagacg gacctcgggc ggcaccgggt
    15601 ttccggcaac ccacgcgggc atcgcctgga cggctttcgg gtcggcgggg ctcgtcacga
    15661 ccctaggata tcagcagagc tgcctttcga tgccaagagt tacgtgcgtg gttttgtcat
    15721 tgcgcaatcg ccaaggcgcg ggctaaaacc ctgccttcac cgctttttcc gtcgaggagg
    15781 cttgccgtga ccgctgtttc gattggctct tttccgctat cccaacgtag cttgcggttg
    15841 ccgctctttt tttcggtgct tgcggttgcc aggtccgcgg cggcgggtgg tttcgaaatt
    15901 ccggccaacg gcaccgaagc actgggtcgt ggcggcgctt tttcagcaaa ggctgactcg
    15961 ccactggcgc tggaatacaa cgtcgccgga cttgcgcagc agcgcggcac ccatgcgctt
    16021 atcgacaaca acctggcgtt ttcgcgctat cgcttcgcgc gggatggcgg tgacagtttc
    16081 ggtccattcc cggcggtgca ggccaatgcg acgccgccgt tttatgcgcc gtggtttggt
    16141 cttgcgacgg actttggctt tttcaaacga ctgacatttg ccgtcggcat ttacggtcca
    16201 tcgtcgattg gcaagcggac gtttccgatg tttgtcccga tggacagcgg caaagatcga
    16261 ccagggcctg cgcgctacga catcgcaacg acggatcttt tgatcttttt tccgacgctg
    16321 gccgttggtg tgcgagcgtc gaagtacctt gacttggggt tttcggtgca gcaggcgtcg
    16381 gcccagatca agctcgcgag catgacgtat gcgccgcaga gtctgccgat ttttccgcag
    16441 tcgagcgctt gttcaatgca ggccgaggtc gcaggctgtg attccctgac gcgcatcacc
    16501 gggtccagct acgacaacta tctgctgcag cttggggcgc tggcgcatct ggggaagtcg
    16561 gttgatctgg gctttaacgt ccgcagcgcg tcgaacctgg gcatccgtcc gattgtggtg
    16621 cgcggcacgg tatccgcgac ggaaccgctg tatttgcaag gcgcgggcat cggcgcagaa
    16681 aaaatggacg gcgagtttac gatcaacctg ccgtgggtgt tccgtggcgg tgttcgctat
    16741 gggcaaaagc tcggcggaac gcagctattc gacgtcgagc tggatgcaat ttacgaagcg
    16801 tggagctggc tcgacgggac ggatcaccag ctcacgctga aaaatccgcc gccgctcgtc
    16861 aacaaagggc agccagtcca gatcacgctg ccacatcact atcgcgacac catcgggctg
    16921 cggcttggtg gatcgctgta tatgccgctg gctgccgatg caggcgtgac gatgcggatg
    16981 ggtggttttt atgacagctc cgcgagcaat gatgccgatc tgcggctcga ctttgacacg
    17041 ctggaaaagc tgggcatctg cgccggtctt ggcttttcgc tgcgtggcgt cgaggtcaat
    17101 tttgcgtatt cgtaccagca ctcgttgccg cgcaccgtgt caaatggcca gctgagcgtc
    17161 atcgacggga cgaccgggca gccggtgcag gttggcggca tgtcgacgcc cgctatcaac
    17221 aacggaacgt acagtggatc gacgcacatc gcttcgatcg gtctgacgct gcgttttgac
    17281 gagtggcgcc cgtcgtataa atccagctca gagggacttt aggtagcgct cgaccgcgtc
    17341 gacaagctcg ctgcgacggc tgcgcaggct gccgacaaag gcgtgatgca cgactccgcg
    17401 ttcgtcgacg agcagcgtca gcggctgggc aaagttctgc aaactgctgc gcagtcgatc
    17461 gtcaatcact tggtacagat cgccgtttgg gaaatagccg cggtgttgct tcaggaactc
    17521 ttgcagcttg ggcacgtcgc gggtttcgct ggcgatgacg agctttagcc tcgggtcttt
    17581 ggcctgcgct tcgaaaaaac tacgcagcgt cggaagttct tccaagcagg gtcggcattc
    17641 gaccgcccac aagtgcacaa aaagcagcct ggcgacggga gcttcggttg cgacaaattc
    17701 gtttttgcgc agcacacgca gcgtcgatag atccatccga cggtgcaaaa gctgggcttc
    17761 ttgccggcgc agaagcagct cttcaggaac cggcgtctgg gcgtggacct gcgcagtctg
    17821 gagtgacaaa agcagggcca aaacctgagc aagcgtgcgc atcagcgttc cccttcgcgg
    17881 ttacttcggc gattcgagcg ggcgcagcgc gggtggtggc agacagtcgt cacagcccgt
    17941 cggctcactt tgttcggtcg gcgttgcaca gtcaattcgg aacacccaga cgctctgtgc
    18001 cagctcgctt ccgacgggcg gcatgtcgtc cggattcagg tattcgcgct tcttgagcga
    18061 aaacgaatac acccagtgca gcacctgatc tcgctttagc ttttgtccgc cgggaaactg
    18121 gatgtcgagc agcttttcga tgatctgcag accgcgccgt tcggcccggg ccacttgggg
    18181 atcttgcgtc gacagcttgc tggttcccgt ctgtcccagc ggcggtggcg gcaggttcga
    18241 cacgaccaga aacttggtgg tttcgagcca tggcggctgc ggctgaatcg tccgcttgag
    18301 cctttgcaaa tacacgcctt ccagtccgtc gatgagcgtg tctttgggcg taaagtacag
    18361 caccgagtgg gtctgtcccg acatcatcgc gacaaagcga cccaggtgcg tcgggtctgc
    18421 gtctccgacc agcgttgcaa ttttctgatc aagacagatg tcttcgcgct tgcagacctg
    18481 cgccacgtcg tcgataagct gccggaccgg aggccgacac agcgcgagcg ccagaccttt
    18541 ttgcagctgc cgctgaccac agacctgaca gcgctgcatc cgctcgacct ggccgcggag
    18601 atccgcaatt tcttccgcgt aacagcttgc ggaaaacacc atcgctgccc cgagcagcac
    18661 ccgccaccgt cgcccactca tggtgtggtc ttcacctttg cctttccggt gatcgtcacg
    18721 actttgggaa tgactatgcc gcttatttgc agttttaggc aatcgcgaaa gtcggagctg
    18781 gtttgatact c
//
LOCUS       DAORGN010000084        18667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1193_length_18667_cov_5.552708, whole genome shotgun sequence.
ACCESSION   DAORGN010000084 DAORGN010000000
VERSION     DAORGN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18667
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1193_length_18667_cov_5.552708"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKL17_10950"
     CDS             <1..356
                     /locus_tag="PKL17_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016495139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK45294.1"
                     /translation="AKPGEPAKPAEAAKPAEAAKPAEPAKPVEAAKPAVPAKPAEAAK
                     PAVPTPPTPPAAPTPAATPAAAPAPVKKKPKKAAEPASDLPPPAEAPSFAPSPEKKEA
                     PPAKEEGSPSAPEKQ"
     gene            356..1072
                     /locus_tag="PKL17_10955"
     CDS             356..1072
                     /locus_tag="PKL17_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45295.1"
                     /translation="MFDRYVQDKRRKRPLLYAAFVISTTIEAGVLIFFIIYSLIHVDE
                     VQPPPLTVQFFATSTPPPPPPPPPKASSKPKTPPKVVKPVVQPTEVPKFIQPKLEPDP
                     PEEKDTGGSSDGVPGGVEGGVAGGVVGAPPPPPPPKEEAKPPPKPKVVPAIRIRSEKL
                     SGEDPHLPAVVKAQRRGSGMVMGAYKMCIGLDGSISDVDVVTSIAGADESIIEVLRTW
                     RYKPQQIPVCFIQNLQFVFE"
     gene            1196..1903
                     /locus_tag="PKL17_10960"
     CDS             1196..1903
                     /locus_tag="PKL17_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK45296.1"
                     /translation="MEQGISFSPKAIWDSTSIAGKAVIIVLAIMGIYMLAVAIERWIT
                     FSRAKQRSLQFVFALQKKLADRDIKGALSLAQVKPQGPIAIVIEAALHEYIDGLQALK
                     TLGPDEIGDFDLLDAVNRAIDRVKEREVADLKKGLGALATIASAAPFVGLLGTVVGII
                     TVFATMSTKGAGGIAAVAGGIGEALVTTAFGLLVAIPAVMVFNFFTNSIDEFVVDMNE
                     TSSELVAFILREGRRAA"
     gene            1957..2481
                     /locus_tag="PKL17_10965"
     CDS             1957..2481
                     /locus_tag="PKL17_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK45297.1"
                     /translation="MSDLDELLKNPPRMPRVKHRRHARHAVKNASIKSEINVTPLVDV
                     VLVLLIIFMVVTPMITRGMPVEQPVTKHHDKKNDSGEQIVVSVTCEGGKMGAIHWECA
                     QSRMFVGSDLATDETLGDLVQKEMRKGSGGREVHIKADRRAAYGVVRHAMELVNAVGV
                     PQVHLGSEEYKEGN"
     gene            2484..2891
                     /locus_tag="PKL17_10970"
     CDS             2484..2891
                     /locus_tag="PKL17_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK45298.1"
                     /translation="MAFAAGGKGPRSDINVTPLIDVVLVLLIVFMVMVPKLLNQTQID
                     VPPKAPPTNMAEPPPSSDQLVATVAEDGRVAINGEAIPIDGLAEKVRTLIQNRSKKII
                     FFNPDDAAPYGDVVKAMDICRGAGVKTLGIMTK"
     gene            complement(2944..3648)
                     /gene="aat"
                     /locus_tag="PKL17_10975"
     CDS             complement(2944..3648)
                     /gene="aat"
                     /locus_tag="PKL17_10975"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNK45299.1"
                     /translation="MARLRLPIWLTEELRFPPLGCASAEGLLAIGGDLQLERLLLAYS
                     TGIFPWPGEGMPLLWFAPPERAVVRPSEIHVGRSLRKVVQRGPYEVRLDTNFAAVIGH
                     CAKIPRRHESGTWILPEMIDAYCALHEAGFAHSVETYQAGELVGGLYGISLGGMFFGE
                     SMFAKADDASKVAMVRLSEQLVAWNFDLIDGQVMNHHLERLGFALIPRAEYMATLRRS
                     LQKPTRRGKWTFSEPG"
     gene            complement(3738..4760)
                     /locus_tag="PKL17_10980"
     CDS             complement(3738..4760)
                     /locus_tag="PKL17_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45300.1"
                     /translation="MSQLDGGQQDDLANLPGVGSAFGYDPGAELKKKGLPWPAYLAIG
                     IAVAALFGFLFFRSYKKREERKVHVAFLEQLQDYEKNTVNAFWRCLFGKEGDGRRFNQ
                     PEQLNGQLEAALYADPKAFPEKVSNECVKKALDASRKVRDFSPPAPAQYEQALDEYGK
                     ALAGLANTLNTWAEGAGKRVETKQRENKITSAGDTWSTTDNPNKADPAAWQYDKFIHC
                     AVPNIDKMKDSQELLEFLASQCTENKAKGWKTNADFLNKIKDTCIAEATGEVPTKTPA
                     TFKATFNKFGADYDRLSQAWGTCLRKMSKESKKDDLEQFDKAWVESVNASTKIRKIAK
                     ENLPEE"
     gene            4984..5595
                     /locus_tag="PKL17_10985"
     CDS             4984..5595
                     /locus_tag="PKL17_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45301.1"
                     /translation="MNSKVKSFFRAATVSTALLLGAVTAGGCGPGWTVVKQAAPSPFT
                     AQSVFSVEPIAYEGLKVGGKSEADYLAGKEPGQVDAWKADLGKLNQAYADNIVKEGKG
                     LQVSVGPAAAPSPFAVKPMVSFIEPGVYAGIVNLPSEVRMTLRVTDAQGAVIDEVATS
                     VRYATQSIMGVPVNPSVTARLTECGKMLGEATAKYLKVRSGVK"
     gene            complement(5623..6477)
                     /locus_tag="PKL17_10990"
     CDS             complement(5623..6477)
                     /locus_tag="PKL17_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45302.1"
                     /translation="MSFAGGLVVSGCSLPEYRLSLANIPDETTHLSTALAIPQADGAA
                     PLIPSERVESDWLDHKSPFRITVNLLDTYDHPPSAVFSAIARDAANCIVATGTTDPVS
                     PSTTVADITLGLKQPTYPRAIQGRCASDGPVVLDILREEAGPFQLTNFRLIARGWDFQ
                     PTDQITVTSLVQIPSSVCRNSGCKGRCPEALDACADPTTKADTTCQTSCSVVVQPKFN
                     GVGQWTIEVPEAGNSIEDPPASGALCCTLQSTNLTTMRGSPFRVTITRKGGTSSTYTE
                     VDPRKKIQ"
     gene            complement(6551..7351)
                     /locus_tag="PKL17_10995"
     CDS             complement(6551..7351)
                     /locus_tag="PKL17_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45303.1"
                     /translation="MIRGTIHNTGLHPIWIILVLVLTTNQAEARRSRSPNPGASSQPA
                     EERTNDAAPKQPSGPHEPAPKTPSASTEPPPSTAAQPAVETPSAGSAAGSQSAPPSKL
                     DPADVDSTLAEATALFKAGQHEQAARKLLLVYEVSPQPILLFNAGQAFRRAKLPRDAK
                     DCYVRFLEAVPQSPLGPEVRGYVRDMDTLIEMQRHEQEISLKLVQEQAAATDAQQALQ
                     KERSKPVYKRAWFWAVLGSVGVAAIAGLSFGAFVGSATQADVKADIRQ"
     gene            complement(7348..8334)
                     /locus_tag="PKL17_11000"
     CDS             complement(7348..8334)
                     /locus_tag="PKL17_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNK45304.1"
                     /translation="MPQLRYQGRSFVLAGETVLERLLAEGISVPNSCRSGVCQSCLLR
                     ATEGKPPENAQQGLKDSWKARGYFLACVARPTEDLTVTSGDEESLNLPAQLTERDWLT
                     PSVLRLRFVCPEPFPYQPGQYVNLVAPSGLVRSYSLASLPTEPFLEFHVRILPDGRMS
                     SWLRTISVGTKAQIRGPAGECFYVPGRPNQPLLLVGVGTGLSPLYGVLREALRQKHDA
                     PITLIHGARTPHGLYLRKELSDIAKTHPNVTYHPVVLSDDGSTDDGTLSRCEVSSLDA
                     FLKTRFAKLSGQRAFLCGDAELVGQLRKQVFLQGCSRKDILADAFVMADTKT"
     gene            8452..9198
                     /locus_tag="PKL17_11005"
     CDS             8452..9198
                     /locus_tag="PKL17_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45305.1"
                     /translation="MSLSSPKPVVPRHAAQRLQRRLCRHIGTLSPGKPDVSVHLTDNC
                     STMLSVRREPGRYRLRLHHMFVSASAQVVAALGRYVIHPEAEASATLDAFIAEQSQHL
                     QPVVRRVLRNPRVIERPDGEHHDLRAIFDDLNAGYFGGSIDAKITWGRRKRTSEPARL
                     HKSLTMGSYSVEDRLIRIHPTLDRPDVPGYYVAWIVYHEMLHQKHGIPERGGRRQFHP
                     PAFVAEERLFDEYERAHRFEREHPHRLLLY"
     gene            9329..10402
                     /locus_tag="PKL17_11010"
     CDS             9329..10402
                     /locus_tag="PKL17_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45306.1"
                     /translation="MSLWFGPAHADDEAPRPRQVPLEAFYDVLREHGTFIDTDRYGTA
                     FCPHPDLVGADFQPYRRGHWVMTEYGWTFTSDLKISWVTDHYGRWVEVGLQNCAWAWV
                     PGGEWGPAWVDFRIGEKVVAWRPSVYSGGKVRLKLPDAKNFPHFNVPEVPQSDSAYVV
                     VREGEFQARRLDYVALTGVAQFNALRETEPVRDLRAGLHSYEYDVLVARKEQKRNQAA
                     QASAQGSQTTSNAQRQAAAAAGGDGSEPAQRRRKGDPATTGAAGTVRAGLPGAELPKN
                     PAAREKLPLAGGQTGDNPPSRTSATTGSSAGNPGEFSGVKVLDWGKPKPQEPAKGTVR
                     DLNRTLSPPSGKTTTPPPPQQQP"
     gene            complement(10409..11551)
                     /locus_tag="PKL17_11015"
     CDS             complement(10409..11551)
                     /locus_tag="PKL17_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNK45307.1"
                     /translation="MHASRAARILGPYVARDKWRLVIIEGRGRKSLIFDSPEQAEAAK
                     STIGQQISQPTLSIGELIELFIADKRRRGLKPLSLRTVQDRLSGFLPIDQPIAAFNYQ
                     AAQKSYDSMQDRFATATHHAALKCAKALFRFAIAHGHARDNPFAGITPLGRPRRGKLQ
                     LSVDEARKLSQALRHEAEQGDELSMALLLQLFLGLRTSEVVQRRVRDLDDRGQVLVIP
                     SGKTEQARRRLECPPFLSELLRKKVAGKTAEQWLFGEQTPHTQAWLWKGLRRHCRALA
                     LPVVCPHSLRGLHSSLAIEAGHTSQEVASQLGHKSFAVTALHYIRPGTIENSRIRKVS
                     SLLATESAPSPGEMSPEKDRLLAILATLSEAELARIVADLPPLKDR"
     gene            11703..12455
                     /locus_tag="PKL17_11020"
     CDS             11703..12455
                     /locus_tag="PKL17_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45308.1"
                     /translation="MKSHRAVVLTGLVAASLQFGAVSVASAQTVVAAPPPASGTIVNP
                     PPPAVGASAPLVQPAQPYYAPPGTVVYPGGQPYPPPQPVYGQPYPPPQPVYGQPQPIQ
                     PPLQPPPVRYEYRRPNKALLIAGISVMGGAYLLTALPTALWNWVDRDLSNSVGSSNHG
                     TYWPLYIPVLGPWIELSYLNSNPYYSGWSVLPAIPCVLSGLVQGAGLAMTIAGAVTGR
                     KVPVSDPQGPTVSFAPISLPNGGGGIGASGRF"
     gene            12504..14198
                     /locus_tag="PKL17_11025"
     CDS             12504..14198
                     /locus_tag="PKL17_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNK45309.1"
                     /translation="MTSHSQLPVLIVGAGPVGLSLACELVRHKVPFRLIDQADGPTIY
                     SKAQILHARTLELYEDLGIATKLCEQARPARAMNMFGQQWERIAHIPIGEHDTAYPYM
                     LNLSQRDTELILAEHLESLGGRIERKVRLSAFERKDDEVVCTLVHEGGRTETLTVRYL
                     CGCDGAHSTVRKGLGLPFVGETYEWRLTQADVRIDWSQSLPTDEAVTFMSPQGLLAAF
                     PLPGERRYRLIAFDAAEEPTLATFQRLLDERGPSGCRVSDPHWMVQFTIHCRQVERYR
                     VGPVFLCGDAAHIHSPAGGQGLNTGVQDAYNLGWKLGLCYHGVASDKLLDSYHEERHP
                     VAAAVLRGTDSATKGAGVLLRLSSEWAQHLRNSAMRLFSSLSLVQRAASRSLSELDVA
                     YSRSPIVGQRQSTPWNRQGDDGHESPGLRDFFVFGDGPAPGARVPDIHFVNARGEDQR
                     LLPALHGGQHHVLLFDGAAATEAGYKNLMMIASSLSARYGDRFVSHIVLPSRERPDEL
                     YHYAGSILFDPRGELHRAFGARSECLYLIRPDGYVGFRSQPASAEALHEFLGRIFS"
     gene            complement(14241..15338)
                     /locus_tag="PKL17_11030"
     CDS             complement(14241..15338)
                     /locus_tag="PKL17_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNK45310.1"
                     /translation="MRVFKPTRVPGGRLLGWLCGALFGLGIFAMAGLPAAAADKRAGK
                     LLKKEAVFTKAAPATVLIVIPGHGKGAIGSGVIVDSSGLVLTNAHVVEMADASVQVFL
                     YNPKEKTLANELSEYVKTHTPLKGTVLRRNRLADLALVQLPLRQASYPTIDLAEQDSL
                     KIGQDVVAIGNPLGLTWTFTTGTVSSLRDDMIQTETPINRGNSGGPLLDLRAKLVGIN
                     TRIKRDSEQTAVFGFAIPVAVADGFLKKWRTRNDSSQEPQISLSKNPVPLLALVLRQD
                     IDRLRQHNAKRGNTNTEQRVERDLVACEKLGAQTLEDDLTVGQVIPRLVEKFHNIAQH
                     NSGAGDGIEQKLQQLTERAIDHLKQIAEVAK"
     gene            15409..16437
                     /locus_tag="PKL17_11035"
     CDS             15409..16437
                     /locus_tag="PKL17_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45311.1"
                     /translation="MRSAIGPAGSNRGAVVQSVALALATAASLVAGCASTPAGLRNLD
                     RACAQGSRTACSRLGIAYYEGKDEAGGPIPLDHRRARQWFEVGCQRGLGESCYFLGTM
                     CLRGEGGPLDGQRGVDLWRRACELGDGLSCRKTAEGYVDGSVGVKSRDLAHAYAKQGC
                     ERGDKPSCAIWKQLGGKPPPSPEVVKLMTEITAACERKGDAKACFALGEIFDQGLESE
                     VNKEKAAQNYRTACEKGEPRGCHQLGVMMIMGEGIPPAPARGLAFLSRACDAKLLPSC
                     DQLLKVLTIQCNRTEPDADACTVLGRLHLKGERGIDTNIIKGVEYLRRGCRGGDNDGC
                     EDLRRLGL"
     gene            complement(16447..17289)
                     /locus_tag="PKL17_11040"
     CDS             complement(16447..17289)
                     /locus_tag="PKL17_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45312.1"
                     /translation="MSARSLSLVCLLSYSTAFAQNKDDPQKERARIVARSLAQEATQS
                     FERRDYGKTQMLLEAAYMLYATEKIQYSLARVYEAQGQKLSAMRSYQQFLRKVPLADR
                     LPGQTEDATGAIARLQKDLAHLLFSRALTAPLRIDDEVPTPKGSIDAWVEPGTHRLLL
                     PHDIEQIKVAAGETRSIEVPAEAAPPITHTDVSSLPPKPTARPGLRTAKWTLGVTGLV
                     ALGVGGTLLGISGLRACANFPTSCNDPLQTKWPGVGLLVGGGVMVGISAVLLGVDAYR
                     PDPQ"
     gene            complement(17289..>18667)
                     /locus_tag="PKL17_11045"
     CDS             complement(17289..>18667)
                     /locus_tag="PKL17_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45313.1"
                     /translation="PQNIFLVRHEIAEMVTEVAKVVDFGISKIRRTGGQMTRDMTLLG
                     TPQFMSPEAALGQNSALDGRADQWSLAVIMYLCLTGRLPFDSENPVGVLYMVVHEKPV
                     PIKQLAPDLPDHIVAAIEKAMSKRKDDRFPRMVDFVKAVLGQTQAAPSRLNLAPAKAT
                     DAQPAQAVERPRVQPSPQPARPLPAALPSVGHMPTGPGAVPAKPGPIGGTPTPQFDPP
                     TVPQTTPSLLPAPTKLAPVRTPSAPSGNTPTNIVLNDAEMQALLSRSATTPVSNDAAA
                     ADFEQVPTRLVGNNDPTPPVPEPGLKAPSLDKPFDPYPSTLSRATGQQSISTGGALVD
                     GSEVTGKNPPVGLLLSTIGVNPRSNLRLGLSGAAIVVCIAILIWLLSGGDSKEQVASG
                     PPAITDTKTASESPDAGISDDKMGGKNSGPTGRAPGGSSPSGSPSKRPTAKPTDTGSK
                     RIKIDPGV"
BASE COUNT         3369 a         5598 c         5920 g         3780 t
ORIGIN      
        1 cggccaagcc cggtgagccc gccaaaccag cggaagcggc caaaccagcg gaagcggcca
       61 agcctgcgga gcccgccaaa ccagtggaag cggccaagcc tgcggtgcca gccaaaccag
      121 cggaagcggc caagcctgcg gtgccaaccc caccgactcc accagcggcg ccaaccccgg
      181 cggcgacccc agcggctgcg cctgctccgg tgaaaaagaa gccgaagaag gctgcggagc
      241 ccgcgtccga tcttccgccc ccggcagaag cgccgagctt tgccccaagt cccgaaaaga
      301 aggaagcccc tcccgccaag gaggaaggta gtccgtctgc gccggagaag cagtgatgtt
      361 tgatcgctac gtacaagaca aacggcgcaa gcgcccactg ctctacgcag ccttcgtcat
      421 ctcgacgacg attgaggctg gagtactgat ttttttcatc atctactcgc tcatccacgt
      481 cgatgaggtt cagccgccgc cgctgacggt gcagtttttc gcaacgtcta cgccgccgcc
      541 gccaccgccg ccgccgccca aggcttcgag caagcccaag acgccgccga aagtggtcaa
      601 gccggtcgtg cagccgacgg aagtgccgaa gttcattcag ccgaagctgg aacccgatcc
      661 tcccgaagaa aaagacactg gcggatcgtc tgacggtgtg ccgggtggtg tcgaaggtgg
      721 tgtcgctgga ggtgtcgttg gtgcaccgcc gccgccgccg ccgccgaagg aagaggccaa
      781 gccaccaccc aagccaaagg ttgtgccggc gatccgcatc cgcagcgaaa agctgtcggg
      841 cgaagatccg caccttccgg cggtcgtcaa agcgcagcgt cgcggcagcg gcatggtgat
      901 gggcgcctac aagatgtgca tcgggctcga cggctcgatc tccgatgtcg acgtggtgac
      961 cagcatcgcg ggcgccgacg agtcgatcat cgaagtgctg cgcacctggc gctacaagcc
     1021 gcagcagatt ccggtctgct tcattcagaa cctgcagttt gtgttcgagt gataaggacg
     1081 gagaacagcc aatcacgagc agcatcggca gaaagtggtg cttgcagggg tgacgcaagt
     1141 tgccacgccg gtgaagccaa ggatttttgg gttacccgcg aagacgagga acaaaatgga
     1201 acagggaatt agttttagcc cgaaggcaat ctgggactcg acgtccatcg ctggcaaagc
     1261 ggttatcatc gtgctggcca tcatgggtat ctacatgttg gccgtcgcca tcgagcgctg
     1321 gattaccttc tcgcgcgcca aacagcggtc gctacagttc gtgttcgcgc tgcagaagaa
     1381 gctggccgat cgcgacatca agggcgcgct cagcctcgct caagtcaagc cgcagggccc
     1441 gatcgcgatc gtcatcgaag ccgcgcttca cgagtacatc gacggactgc aggcgctcaa
     1501 gacgctggga ccggatgaaa ttggcgactt cgacctgctg gatgcggtca atcgggcgat
     1561 cgaccgggtg aaagagcgtg aagtggcgga tctcaagaag ggtcttggcg cactcgcaac
     1621 cattgcttcg gcggcaccgt tcgtcggtct gctcggaacc gtcgtcggta tcatcaccgt
     1681 ctttgcgacc atgtcgacca agggcgcagg cggtatcgcg gcagttgctg gtggtatcgg
     1741 tgaagcactc gtgacgacgg cattcggcct tctggtcgca atcccggcag ttatggtgtt
     1801 caacttcttc accaactcga tcgacgagtt cgtcgtggac atgaacgaaa cgagcagcga
     1861 gctggtggcg ttcatccttc gcgagggtcg tcgggctgcg tagtcgtacc cgtgcggtgg
     1921 tttgccatcg cgcctagata agctgaagga ctgcccatgt ccgacttgga tgaactgcta
     1981 aaaaatccgc cgcggatgcc gagagtcaag caccggcggc acgcgcgtca tgcggtcaaa
     2041 aatgcgtcga tcaaatcaga gatcaacgtg acaccgctcg tcgacgtcgt gctcgtgctg
     2101 ctcatcatct tcatggtcgt cacgccgatg atcacccgtg gtatgcccgt cgagcagccg
     2161 gtgaccaagc accacgacaa gaagaacgat tcgggcgagc aaatcgtcgt ttcggtgacc
     2221 tgcgaaggcg gcaagatggg cgcgatccac tgggaatgcg cgcagtcgcg gatgttcgtg
     2281 ggatcggact tggcgacgga cgaaacgctc ggcgatctgg tgcaaaaaga aatgcgcaaa
     2341 ggatcgggcg gacgtgaagt ccacatcaag gccgaccgtc gtgctgccta cggcgttgtt
     2401 cgtcacgcga tggagctggt caacgccgtc ggcgtgccgc aagttcacct tggctctgaa
     2461 gagtacaagg agggtaacta gccatggctt ttgcagccgg tgggaaaggc ccacgctccg
     2521 acatcaacgt cacgccgctt atcgacgtcg tgctcgtgct cctgatcgtg ttcatggtca
     2581 tggtgccgaa gctgctcaac cagacgcaga tcgacgtgcc accgaaggcg ccgccgacca
     2641 acatggccga gccgccgccg tcgtcagatc aactggttgc gaccgtcgcg gaagatggtc
     2701 gtgtcgccat caatggcgaa gcgatcccga tcgatggtct ggccgaaaag gttcgcaccc
     2761 tgattcagaa ccgcagcaag aagatcatct tctttaaccc cgacgatgca gcgccctatg
     2821 gcgatgtcgt caaagcgatg gacatctgtc gtggcgcggg cgtaaagacc cttggcatca
     2881 tgaccaagtg atccgctagc gatcgccgct tgctcgctct ctccgcattc cgttcgcttc
     2941 tttttacccc ggttccgaaa acgtccactt gccgcgccgc gttggctttt gcagcgatcg
     3001 cctgagcgtc gccatgtatt cggcgcgcgg aatcagcgca aaacccagtc gttccaggtg
     3061 gtgattcatc acctgtccgt cgatcaagtc gaaattccag gccacaagct gctcggacag
     3121 gcggaccatc gcgactttgc tggcgtcgtc agctttggca aacatcgact cgccaaaaaa
     3181 catgccaccg agcgagatgc cgtacagacc gccgaccagc tcgcccgctt gatacgtttc
     3241 gaccgagtgg gcaaagccgg cttcgtgcag cgcacagtag gcgtcgatca tctcggggag
     3301 aatccaggtg cccgattcgt gtcgacgggg aatcttggcg cagtgaccga tgacggcggc
     3361 aaagtttgtg tcgaggcgaa cttcatacgg gccgcgctgg acgactttgc gcagactgcg
     3421 tccaacatgg atttcactcg gacggacgac ggcgcgttcc ggtggggcaa accacaaaag
     3481 cggcatgcct tcacccggcc acgggaaaat ccccgtcgaa tacgccaaaa gaagccgctc
     3541 gagctggaga tcgccaccta ttgccagaag gccttcggcg gaagcgcagc caagcggtgg
     3601 aaaacgcagt tcctcggtga gccagatcgg aagccgtagt cgtgccaagc gcaccaaatg
     3661 gtgccgtgtg tgcgctggct tggcaatgtg taggatgtgt gcggggcggg tttgccgagg
     3721 cgggctgggg acaaaaacta ttcttcgggg aggttctctt tggcgatctt gcggatcttg
     3781 gtgctggcgt ttaccgactc gacccacgct ttgtcaaact gctccagatc gtcttttttc
     3841 gactctttgc tcatcttgcg caggcaggtt ccccatgcct ggctcagacg gtcgtagtcg
     3901 gccccgaact tgttgaacgt cgccttgaag gtcgccggag tcttggtcgg cacttcgccc
     3961 gtcgcctcgg cgatgcaggt gtctttgatc ttgttcaaga aatcggcgtt ggtcttccag
     4021 cccttggcct tgttttcggt gcactggctg gcgaggaact caagcagctc ctgactgtcc
     4081 ttcatcttgt cgatgttcgg caccgcgcag tggatgaact tgtcgtactg ccacgccgct
     4141 ggatcggctt tgtttgggtt gtcggtcgtg ctccaggtgt cacccgccga cgtgatcttg
     4201 ttttcgcgct gcttggtttc gacgcgcttg ccggcgccct cggcccaggt gttgagcgtg
     4261 ttggccagac cagcgagcgc cttgccgtac tcatcgagcg cttgctcata ctgagccggc
     4321 gcgggcggcg aaaagtcacg aaccttgcgc gacgcatcaa gcgccttttt cacgcactcg
     4381 ttcgagactt tttcggggaa cgctttggga tcggcgtaca gcgccgcttc gagctggccg
     4441 ttgagctgtt cgggctggtt gaagcgacga ccatcgcctt ctttgccaaa cagacagcgc
     4501 cagaaggcgt tgacggtgtt cttttcgtag tcttgtagct gttcaaggaa cgcgacgtga
     4561 actttgcgct cctcccgctt tttgtagctg cggaagaaca ggaatccgaa aagcgccgcc
     4621 actgcgatac caatcgccag gtatgcaggc cacggcaggc ctttcttctt cagttcggcg
     4681 cccggatcgt agccaaacgc gcttccaact ccgggcaagt ttgcgagatc gtcctgctga
     4741 ccaccgtcta gttgactcat ggtccccatc tttgatcggt gcgcttgggc ttgtgaagtc
     4801 tgttttgggg ccaagatccc gagcctgacc gcagcggcgc tatctgctcg acatgcttga
     4861 cgttcgccgc cgccttcgcg tttcatgatt tgtaattcgt ggtcgttgga tcgcgggatc
     4921 gcttcggtct tgacagccac acaacgaacc gctcaaccag cggaagcaac aagaggattg
     4981 gtcatgaaca gcaaggtgaa gagctttttc cgtgcggcaa ctgtttcgac ggcgctgctc
     5041 cttggcgcag tgacggcggg gggctgtggt ccgggctgga cggtggtcaa gcaagcggcg
     5101 ccgagcccgt tcactgctca gtcggttttt tctgttgaac cgatcgctta tgaaggcctg
     5161 aaagtcggcg gcaagtcgga agccgattac ctggctggca aggagccggg gcaggttgat
     5221 gcgtggaagg ccgatctggg caagctgaac caggcttacg ccgacaacat cgtcaaggaa
     5281 ggcaaaggcc tgcaggtttc cgttggacct gccgccgcgc cgagcccgtt tgcggtcaag
     5341 ccgatggtgt cgttcattga accgggcgtc tacgccggca tcgtcaacct gccctccgag
     5401 gttcgcatga ccctgcgcgt caccgatgct cagggcgcgg tgattgacga agtcgccacc
     5461 tcggttcgtt acgccaccca gagcatcatg ggcgtgccgg tgaacccgag cgtgacggct
     5521 cgtctgaccg agtgcggcaa gatgcttggc gaagccaccg ccaagtacct caaagtccgc
     5581 tctggcgtaa agtaacccgc ctcatcttcc gctccctcgg cgttactgga tcttcttccg
     5641 cgggtccact tcagtgtacg tcgaggatgt tccgcccttc cgtgtgatcg tcactcgaaa
     5701 tggactgccg cgcatggtcg tcaggttcgt tgactgcaag gtgcagcaca gagcccccga
     5761 tgctggtggg tcctcgatgc tgttgccggc ctcgggaacc tcgatcgtcc actggccgac
     5821 accgttgaat tttggctgta cgacgacgct acaactcgtc tgacaggtcg tatcggcttt
     5881 cgttgttgga tcggcgcatg cgtcgagcgc ttccgggcag cggcccttgc acccagaatt
     5941 ccgacaaacg gaggacggga tctgcaccag gctggtgacg gtgatctggt cggttggctg
     6001 gaagtcccag ccgcgagcga tgagccggaa attggtgagt tggaacggtc ccgcttcctc
     6061 tcggagtatg tcgaggacaa ccggcccgtc gctggcgcac cgcccttgga tcgctctcgg
     6121 ataggttggt tgcttgagcc ccaatgtgat gtcggcgacg gtagtggatg ggctgactgg
     6181 gtccgtggtg ccggttgcca cgatgcagtt ggctgcatcg cgggcgatgg cgctgaacac
     6241 ggcgctcggc gggtgatcgt atgtgtcgag caggttgacg gtgatgcgga acggcgactt
     6301 gtgatcgagc cagtcggatt cgactcgctc actcggaatc aggggggccg caccatcggc
     6361 ttgcggaatc gctagcgccg tcgagaggtg ggtcgtttca tccggaatgt ttgcaagcga
     6421 aagtcggtac tcaggaaggg aacaaccgga aaccaccagt ccaccagcga acgacacaag
     6481 cagcgcgaga cgtttcttgc tgagacatcg gaatgacatc ggaagaccct ccctcgacgt
     6541 gccagtgtct ttactggcgg atgtcggctt ttacatcggc ctgcgtcgcc gaacccacga
     6601 acgcgccgaa cgacagaccg gcgattgctg caaccccgac gctgcccagg acggcccaga
     6661 accacgctcg cttgtagacc ggcttgctgc gttctttttg cagcgcttgc tgggcgtccg
     6721 ttgctgcggc ttgctcttgc acgagtttta gcgaaatttc ctgctcgtgc cgctgcattt
     6781 ctatcagcgt gtccatgtcg cggacgtaac cccgcacctc gggaccgagc gggctctgtg
     6841 gcaccgcttc gaggaatcgg acgtagcagt cttttgcatc ccgcgggagc ttggcgcggc
     6901 ggaaggcctg ccccgcgtta aacagcagga tcggctgcgg gctgacttcg tagaccagca
     6961 gcagcttgcg cgcggcctgt tcgtgttgtc cggctttgaa cagtgctgtg gcttcggcca
     7021 gcgtcgaatc gacatccgcg ggatctagct tgctcggtgg agcactctgg ctgcccgcgg
     7081 ctgatccggc gcttggggtc tcaaccgccg gctgtgcggc tgtgctcggc ggcggttccg
     7141 tgcttgcaga aggggtcttg ggcgctggct cgtgcggtcc ggatggttgt ttcggcgcag
     7201 cgtcgttcgt tcgttcttcc gcgggttgac tgcttgcgcc tgggtttggg gatcgcgaac
     7261 ggcgcgcttc cgcctgattc gtcgtcagca ccagcaccag gatgatccag atcgggtgca
     7321 ggccggtgtt gtgaatcgta cctcgaatca tgttttggta tcggccatta caaatgcgtc
     7381 agccaggatg tctttccgac tgcagccttg caaaaagacc tgtttgcgga gctgaccgac
     7441 gagctcggcg tccccgcaca gaaacgcacg ctggcccgac agcttggcaa accgcgtctt
     7501 gaggaacgca tcaagcgacg acacctcgca gcgcgacagc gtcccatcat cggtgcttcc
     7561 atcgtcgctg agcaccaccg gatgataggt cacattcgga tgcgtttttg cgatatccga
     7621 aagctctttg cgcaggtaca gaccgtgtgg cgtccgcgcg ccgtggatca gcgtgatcgg
     7681 cgcgtcatgt ttttgccgaa gcgcttcacg aagcacgcca tacagcggcg acagtcccgt
     7741 gcccacaccg acgagcaaca gcggctgatt cgggcgtccc ggcacataga aacactcgcc
     7801 ggcggggcca cggatctgcg ccttggttcc gacagaaatc gtgcgcagcc acgacgacat
     7861 gcgaccgtcc ggcaagatgc gcacatgaaa ttccagaaac ggctcggtcg gtagcgacgc
     7921 cagcgaatac gagcgcacaa gcccacttgg cgccaccaag ttcacgtact gaccgggctg
     7981 atacggaaag ggctctggac agacaaatcg cagccgcaac accgacggtg taagccagtc
     8041 gcgttccgtc agttgggccg gcaggttgag cgattcttca tcgccgcttg tgaccgtcaa
     8101 gtcctcggtc ggacgcgcca cgcacgccaa aaaatacccg cgcgctttcc acgagtcctt
     8161 gagcccttgc tgcgcgtttt ctggcggctt tccttcggtg gcgcgcaaaa ggcacgactg
     8221 acagacaccg cttcgacacg agttggggac cgagattcct tcggcaagca accgctcgag
     8281 cacggtttcg ccggcgagca caaacgaacg accttggtaa cgcagttgcg gcatggagaa
     8341 aatattctgc caaaatgcgc atttttggca cgtcttgtaa aaaggccgcg caaatccctg
     8401 gagcagcgct acattttttg acttcggttc tgggccaagt agaacggctg catgtctctg
     8461 tcttcgccca agccggtcgt gccgcggcat gcggcccagc gcttgcagcg acggctttgt
     8521 cgtcacatcg gaacgctgtc gcctggcaag ccggacgtgt ctgtgcacct gaccgacaac
     8581 tgctcgacga tgctttccgt tcggcgcgaa ccgggtcgct atcggctaag gcttcatcac
     8641 atgtttgtgt ccgcgtcggc gcaagtggtg gcggcgctcg ggcgttatgt gattcatccc
     8701 gaagcagagg cgtcggcgac gcttgatgcc ttcatcgccg agcagtcgca gcacttgcag
     8761 cccgtggtcc ggcgggtctt gcgcaatccg cgggtcatcg aacgacctga tggtgaacac
     8821 catgacttgc gggcgatttt tgatgatcta aatgcgggct atttcggcgg cagcatcgac
     8881 gcgaagatca cctggggacg gcgcaaacga accagcgaac cggcgcggct ccacaagtcg
     8941 ctgaccatgg gttcgtattc cgtcgaagat cgcttgattc ggattcatcc gacgctcgat
     9001 cgtcccgacg tgcctggcta ctacgtcgcg tggattgtct atcacgaaat gctgcaccaa
     9061 aagcacggca tccccgagcg tgggggacgg cggcagttcc atcctccggc gtttgtcgcc
     9121 gaagagcgtc tgttcgatga gtacgagcgc gctcatcgtt tcgaacgcga gcatccccac
     9181 cgcttgctgt tgtactgacg gggcagcagc gaagagattg gaagcgttgc gcgtttccag
     9241 gcatcataga aacaacatgg cgcgctggcc tacaatcgct gctccgcgac gcaacttggc
     9301 acgggtggcc ttccctgtgg ctttcgctgt gagcctttgg tttgggcctg cccatgcaga
     9361 cgacgaagcg ccgcgaccgc gccaggtgcc gctggaagcg ttttacgacg tgctgcgtga
     9421 acacggcacg ttcatcgaca ccgaccgcta tggcacggca ttttgtccgc atccggatct
     9481 ggtgggggcc gactttcagc cctatcgccg tggtcactgg gtgatgaccg aatacggctg
     9541 gacgtttaca agcgatctca aaatcagctg ggtcacggac cactacggtc gctgggtcga
     9601 agtcgggctg caaaactgcg cctgggcctg ggtgcccggc ggagagtggg gccctgcgtg
     9661 ggtcgatttt cgcatcggtg aaaaagtcgt ggcgtggcga ccgtcggtgt attccggtgg
     9721 caaagttcgt ctgaaactgc ccgacgccaa aaactttccg cactttaacg tccccgaagt
     9781 tccgcaaagc gattccgctt atgtcgtcgt ccgcgagggg gagtttcagg cccggcggct
     9841 cgattacgtg gcgctgaccg gagtggcgca atttaacgcg ctgcgtgaaa ccgagccggt
     9901 gcgcgatctg cgggcggggc tgcactcgta tgaatacgac gtgctggtcg cgcgcaaaga
     9961 acagaagcgc aaccaagcgg cgcaggcttc agcgcagggg tcgcagacca catcgaatgc
    10021 gcagcgacag gcggcagccg cagccggggg ggatggttct gaaccggcgc agcgtcggcg
    10081 caaaggcgat cctgccacca ccggggcggc tggcacggtg cgggccggac tgcctggcgc
    10141 ggagctgccg aagaatccgg cggcgcgtga aaagctaccg cttgctggtg gacagacagg
    10201 cgataatccg ccgagccgaa ccagcgcgac caccggcagc agcgccggca atccgggtga
    10261 atttagcggt gtcaaagtgc tcgattgggg caagcccaaa ccgcaagaac ccgccaaagg
    10321 caccgtgcgt gatttgaacc gcacgctatc gccaccgtcg ggcaagacca cgacaccacc
    10381 gccaccgcag cagcagccgt aagtttgctt agcggtcctt gagcggcggc agatcggcga
    10441 cgattcgggc cagttctgct tcgctcagcg tcgccaaaat cgcgagcagc cggtcttttt
    10501 ccgggctcat ttcgccgggg cttggcgcgg attccgtcgc aagcaggctg ctgactttgc
    10561 gaatgcggct gttttcgatc gtgcccggac ggatgtagtg cagggcggtg acggcgaagc
    10621 tcttgtgacc aagctggctg gcgacttctt ggctggtgtg tcccgcttcg atggccagcg
    10681 acgagtgcag accgcggagg ctgtgcggac agaccacggg aagtgccagc gcgcgacagt
    10741 gccgacggag acctttccaa agccaggcct gggtatgcgg cgtctgttcg ccaaagagcc
    10801 actgctcggc ggtcttgccc gcgacttttt tgcgcaaaag ctcgctcaga aacggcggac
    10861 actccagtcg tcgccgcgcc tgctcggttt tccccgacgg aatcaccagc acttggccac
    10921 ggtcgtcgag atcgcgcacc cgcctttgca ccacttcact tgtgcgcagc ccgaggaaaa
    10981 gctgaagcag cagcgccatc gaaagctcgt ccccttgctc ggcttcgtgg cgcagcgctt
    11041 ggctcagctt gcgggcttca tcgacggaaa gctggagctt gccgcgccga ggccgcccca
    11101 gaggtgtaat gcccgcaaac ggattgtcgc gtgcatgtcc gtgggcgatg gcaaaacgaa
    11161 acagcgcttt ggcgcatttg agtgccgcgt ggtgcgtcgc cgtcgcaaaa cgatcctgca
    11221 tcgaatcata cgatttttgc gctgcctgat aattgaaggc ggcgatcggc tgatcgatcg
    11281 gcaaaaaacc actgagtcga tcctgcaccg tgcgcagcga cagcggcttc aagcctcggc
    11341 gacgcttgtc ggcaataaat agctcgatca gctcaccaat cgagagcgtt ggttgggaaa
    11401 tttgctgccc aattgtcgac ttggcggctt cggcctgctc gggactgtca aagatcaggc
    11461 tcttgcgtcc tcggccttcg atgatgacca gtcgccactt gtcgcgagcc acatagggcc
    11521 ccaagattcg cgctgcccgg cttgcgtgca tcctcttaaa atcacacaaa attttttgca
    11581 gataaagcct gactcgcgcg cgggctgcgg cttgcgcctg gcatcgatac acggcttcgc
    11641 ggggcggctc ttgattcgct gtgtggctgg ggtcatcctg ggctgtagga aggagtccga
    11701 acatgaagtc gcaccgcgcg gtcgttttga ccggccttgt cgctgcctcg ctccaatttg
    11761 gcgctgtctc tgtcgcatct gcccagacgg tggttgctgc cccaccgcca gcgtcgggca
    11821 cgatcgtaaa cccacctccg cctgcggttg gtgcaagcgc gccgctggtt cagcccgcgc
    11881 agccgtacta tgcgccgccg ggcaccgtcg tctatccagg cgggcagccg tatccgccgc
    11941 cgcagcctgt gtacggacag ccgtatccgc cgccgcagcc tgtgtacgga cagccgcagc
    12001 cgatccagcc gccgcttcag ccgccgcctg tgcgttacga ataccgtcgt cccaacaaag
    12061 cgctgctgat tgccggcatt tcggtgatgg gtggagcgta ccttttgacg gctctgccga
    12121 cggcgctgtg gaactgggta gaccgtgacc tgagcaactc ggtcggatcg agcaaccacg
    12181 gcacgtactg gccgctctac atcccggtgc tgggtccgtg gatcgagctg agctacctca
    12241 attccaatcc gtactacagc ggctggtcgg ttctgccggc gattccttgc gtgctgtcgg
    12301 gcttggtgca gggcgcgggt ctggcgatga ccatcgcggg cgcggtgacg ggtcgcaagg
    12361 tgccggtttc cgatccgcag ggtccgacgg tgagctttgc tccgatctcg cttccgaacg
    12421 gcggtggggg cattggcgcg tcgggtcggt tctaagcggt ccgctacacg tcgcgacatc
    12481 gtcttcggag ggggggaggg aacatgactt cgcattctca gttgccggtt cttatcgtcg
    12541 gggccggccc ggtggggctt tcgcttgcgt gcgagcttgt gcggcacaaa gtcccgtttc
    12601 ggctgatcga tcaagccgac gggccgacga tttactcgaa agcgcagatc ctgcacgcgc
    12661 gcacgctcga gctgtacgag gatctgggga ttgcgaccaa gctgtgcgag caggcgcggc
    12721 ccgcgcgggc gatgaacatg tttggtcagc aatgggaaag aattgcccac attcccattg
    12781 gcgagcacga cactgcgtat ccgtacatgc tcaacctgtc gcagcgtgac acggagctta
    12841 tcctggccga acacctggaa agcctgggcg ggcgcatcga gcggaaagtt cgtctgagtg
    12901 cgttcgagcg aaaagatgac gaggtcgtct gtacgcttgt gcacgaaggc ggacggaccg
    12961 aaacgctgac ggtgcgctat ctatgcggtt gtgatggggc gcatagcacg gtgcgcaaag
    13021 ggctcgggct gccatttgtc ggcgaaacct acgagtggcg tctgacccaa gcggacgtgc
    13081 gaattgactg gtcgcagtcg ctgcccaccg acgaagccgt cacgtttatg tcgccgcagg
    13141 gcttgctggc cgcatttccg ctgccgggtg agcgacgcta tcggttgatt gcgtttgacg
    13201 ccgccgaaga gcctacgctg gcgacgtttc agcggctgct cgacgagcgt ggtccgtccg
    13261 gctgtcgcgt aagcgatccg cactggatgg tgcagtttac gattcactgt cggcaggtcg
    13321 aacgttatcg cgtcggtccg gtcttcctgt gcggcgacgc ggcgcacatc cacagtccgg
    13381 caggcgggca ggggctgaac accggcgtgc aggatgctta caacctcggc tggaagctgg
    13441 ggctttgtta tcacggcgtc gcgagtgaca agctgcttga ttcgtatcac gaggagcgac
    13501 atccagtggc ggcggcggtg ctgcgtggaa ccgattcagc gacgaagggt gcaggtgttc
    13561 tgctccggtt gtcgagtgaa tgggctcagc atctgcgcaa cagtgcgatg cggctgttta
    13621 gcagtctgtc gttggtgcag cgggcggcgt cgcgttcact gtccgagctc gatgtcgcct
    13681 acagccggag tccgatcgtc gggcagaggc agtcgacccc gtggaatcgg cagggcgacg
    13741 acggccacga atcccccggt ctgcgcgact ttttcgtatt tggcgacggt ccggcaccgg
    13801 gcgcgcgggt tcccgacatt cattttgtga atgcccgcgg cgaagatcag cggcttttgc
    13861 ccgcgctgca tggtggacag catcatgtcc tgctgttcga tggggccgca gcgacggaag
    13921 ccggctacaa aaatctgatg atgatcgcgt cttcgctgtc ggctcggtac ggggatcgct
    13981 tcgtcagtca catcgtcctg ccaagccgcg agcgtcccga cgagctgtat cactatgcgg
    14041 gttcgatcct gtttgacccg cgtggcgagc tgcaccgtgc gtttggcgcc cgcagtgagt
    14101 gtctgtacct gattcgtccc gacggttatg tcggattccg cagccagccg gcctcggcag
    14161 aagcgctgca cgagttcctc ggtcggatct tttcctaacg ctgccctttt tccccagtgc
    14221 agggtcgttt cgcagcgatg ctacttggcg acttcggcga tctgctttag gtgatcgatc
    14281 gcgcgttccg taagctgctg gagtttttgc tcgatgccgt cgcccgcgcc gctgttgtgc
    14341 tgcgcgatgt tgtgaaactt ttcgacgagc cgcggaatca cttgtcccac cgtcagatcg
    14401 tcttcgagcg tctgcgcacc cagcttttca cacgccacaa gatcccgttc gacccgctgt
    14461 tcggtgtttg tgttgccgcg cttggcgttg tgctggcgca gtctgtcgat gtcctgacgc
    14521 agcaccagcg cgagcagcgg caccggattt ttcgataggc ttatctgcgg ctcttgggac
    14581 gagtcgttgc gggtccgcca ttttttcaaa aagccgtcgg ccactgcgac aggaatcgcg
    14641 aaaccgaaga ccgcagtctg ttcgctgtcg cgcttgatcc ttgtgttgat accgaccagc
    14701 ttggcccgca aatcgagcag cggaccgccc gagttgccgc ggtttatcgg cgtttcggtc
    14761 tggatcatgt catcgcgcag cgacgaaact gtgccggtgg taaacgtcca cgtcagcccg
    14821 agcggattgc cgatggcgac gacatcttgg ccaattttta gcgaatcctg ctcggccaga
    14881 tcgatcgtcg gatagctcgc ttgacggagc ggcagctgga ccagcgccag atccgcgagc
    14941 cgattgcgcc gcagcaccgt gcctttgagc ggcgtgtggg tctttacgta ttccgacagc
    15001 tcgttggcga gcgttttttc tttcgggttg taaagaaaca cctggacgct ggcatcggcc
    15061 atttcgacga cgtgcgcgtt ggtcaggacc aaaccgctcg aatcgacgat gacgccgctg
    15121 cctatcgcgc ccttgccgtg gccgggaatg acgatcagca ccgtcgctgg cgccgctttg
    15181 gtgaacaccg cttctttttt cagaagcttg cctgcgcgct tgtccgcagc cgcagcgggc
    15241 agtcccgcca tcgcaaaaat tcccaaaccg aagagcgcgc cgcacagcca gccgaggagc
    15301 cttcccccag gaacccgcgt cggtttgaaa actcgcacaa gtacatcata tcgcggcccg
    15361 cacgtttccc gctgccctcg ataagacatc ttgctaccct gagagacaat gagatccgcg
    15421 atcgggcctg cgggctcgaa caggggggcg gtggtccagt cggttgcgct cgctttggcg
    15481 acggcagctt cgcttgttgc gggctgtgcg tcgacaccgg ctggcttgcg caacctcgac
    15541 cgggcctgtg cacaaggcag tcggacggcg tgcagtcggc tcggcatcgc gtactacgaa
    15601 ggcaaagacg aagcgggcgg accgattccg ctcgatcatc ggcgggcccg gcagtggttc
    15661 gaggtcggct gccagcgtgg gctcggcgag agctgttact ttttgggcac gatgtgtctg
    15721 cgcggtgagg gcggacctct cgatgggcag cgcggcgttg atttgtggcg acgggcctgc
    15781 gagctgggcg acggactttc atgccgcaaa actgccgaag gctacgtcga cggatcggtg
    15841 ggggtaaaga gtcgcgatct tgcgcatgcc tatgccaaac agggctgcga gcgcggcgac
    15901 aaaccaagct gtgcgatttg gaaacagctt gggggcaagc caccaccgtc gcccgaagtg
    15961 gtcaagctga tgacagaaat caccgccgcg tgcgagcgca aaggcgacgc caaagcgtgt
    16021 tttgcactcg gtgagatttt tgaccaaggg ctcgaaagcg aagtgaacaa agaaaaggcg
    16081 gcgcagaact accgtaccgc ctgcgaaaaa ggcgagccgc gcggctgtca ccagctcggg
    16141 gtcatgatga tcatgggcga ggggattccg ccggcgcccg cgcgtggtct tgcctttttg
    16201 agtcgggcct gtgacgcaaa gcttctgcca agctgcgatc agctgctcaa ggtgctcacc
    16261 atccagtgca accgaaccga acccgacgcc gatgcttgca cggtgctggg ccgcctgcat
    16321 ttaaagggcg agcgcggcat cgacaccaac atcatcaaag gcgttgaata cctgcggcgc
    16381 ggctgtcgag gcggcgataa cgacggctgc gaagatttgc ggcgattggg gctttgatgc
    16441 gctgtgttac tgggggtcgg gtcgatacgc atcgacgccc agcagcaccg ccgaaatgcc
    16501 caccatgacg ccgcccccca cgagcagacc gacgccaggc cacttggttt gcagcggatc
    16561 gttacagctt gtggggaagt ttgcgcaggc ccgcaaaccg ctgatgccaa gcagcgtccc
    16621 tccgacgccc agcgcgacaa gtcccgtgac cccgagcgtc cattttgcgg ttcgcagccc
    16681 tggccgtgcc gtcggcttgg gcggcaggct gctcacgtcg gtgtgggtga tgggcggcgc
    16741 tgcttcggcg ggaacttcta tcgaccgtgt ttcccccgcc gcgactttga tttgttcgat
    16801 gtcatgtggc agcaaaagtc ggtgcgtgcc gggctcgacc caggcgtcga tgctgccttt
    16861 gggcgtcggt acttcgtcgt cgatgcgcag cggtgcagtg agcgcgcgcg aaaacaaaag
    16921 gtgcgccagg tctttttgca gccgtgcgat ggcccccgtc gcgtcttcgg tctgtcccgg
    16981 cagccggtcg gcgagcggca ctttgcgcaa aaactgctga tacgatcgca ttgccgacag
    17041 cttctgccct tgtgcttcgt acacacgcgc caggctgtac tgaatcttct cggtggcgta
    17101 gagcatgtac gccgcttcga gaagcatttg ggtcttgcca tagtcgcgtc gctcgaacga
    17161 ctgggtcgct tcctgggcca gcgatcgtgc gacgatccgc gcacgctctt tctgcggatc
    17221 gtctttgttt tgggcaaatg ccgtcgaata gctgagcaga cagaccagcg acagcgaccg
    17281 ggccgacatt agacccccgg atcgattttg atgcgtttgc tgccggtatc ggtgggcttt
    17341 gccgtcggac gcttgctggg cgatccgctc ggcgatgagc cacctggcgc gcgacctgtg
    17401 gggccgctgt tttttccacc cattttgtcg tcgctgatgc cggcatcagg actttcgctg
    17461 gcggtcttgg tgtcggtgat ggccggcggg ccacttgcga cttgttcttt gctatcgcca
    17521 ccgctcagca gccaaatcag gatggcgatg cagacgacga tggccgcgcc gctcagaccg
    17581 agccgcaggt tcgatcgcgg gttgacgcca atcgtcgaaa gaagcagacc gaccggcggg
    17641 tttttgccgg tgacttcgct gccgtcgacg agcgcaccgc ccgtcgagat cgactgctgc
    17701 ccagtcgcgc gcgacagcgt gctcggatag ggatcaaatg gcttgtcgag agacggcgct
    17761 ttcagtccag gttctggcac gggcggcgtc ggatcgttgt tgccgacgag ccgcgtgggc
    17821 acctgttcaa aatcagcggc ggcagcgtca tttgataccg gcgtcgtcgc tgagcggctc
    17881 aaaagcgcct gcatctcggc atcgtttagc acgatgttgg tcggggtgtt gccgcttggc
    17941 gcgcttggcg tgcgcactgg ggccagcttg gtcggggccg gcaaaagcga cggcgtcgtc
    18001 tgcggcaccg tcggtggatc aaactgcggc gtcggcgttc ccccgattgg gcccggcttg
    18061 gccggcactg cgcctggtcc agtcggcatg tgtccgacgc tgggcagcgc tgccggcagc
    18121 ggacgtgctg gctgtggcga tggctgaacc cgcgggcgtt ccaccgcttg ggcgggctgg
    18181 gcatcggttg ctttggccgg ggccaggttc agccgcgacg gtgccgcctg cgtctgtccg
    18241 agcaccgcct tgacaaaatc gaccatccgc ggaaagcgat cgtccttcct tttggacatc
    18301 gctttttcga tcgccgcgac gatgtggtca ggcaagtccg gcgccagttg tttgatcggc
    18361 accggctttt cgtggaccac catgtacagc acgccgacgg gattttcgct gtcgaacggc
    18421 agccgcccgg tcaggcacag gtacataatg accgccagcg accactggtc ggctcgtccg
    18481 tcgagcgccg agttttgacc gagcgccgct tcgggcgaca taaactgcgg tgtccccaaa
    18541 agcgtcatgt cgcgggtcat ctgcccgccg gtgcgacgga tcttcgagat gccgaaatcc
    18601 acgaccttgg ccacctcggt caccatttcc gcgatctcgt gccgcaccaa aaaaatgttc
    18661 tggggct
//
LOCUS       DAORGN010000085        18637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1199_length_18637_cov_6.180713, whole genome shotgun sequence.
ACCESSION   DAORGN010000085 DAORGN010000000
VERSION     DAORGN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18637
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_18637_cov_6.180713"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKL17_11050"
     CDS             <1..403
                     /locus_tag="PKL17_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNK45314.1"
                     /translation="IGLYGGQAQGAVGFSFGVERLIDLLKEQVAGQLQTARPVLVAPL
                     GVGRIADAERVAMTLRQAGIATRLSLSATAPGKHLQYAESVSIDHVIFVGEDEVKQGL
                     YPCKQLSTRSEARLPIDGLSGHLRAAGVLTL"
     gene            complement(393..1082)
                     /locus_tag="PKL17_11055"
     CDS             complement(393..1082)
                     /locus_tag="PKL17_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNK45315.1"
                     /translation="MWRPFWEIWNALWLLLGRHECAACEEPLAEGYRQGPLCFDCFCR
                     CEANPDEPTDLPPDAPPLRSAFAYDGPLSHALLLLKWQGRDDLALPLGRLLTPLLAEL
                     ATQHDWIVPVPLHRSRLRERGYNQSTLLAQEALRAVAPPAARLHPGLLVATRATTAAH
                     HLGRAARLARVRSRFGVPKAAEAKVRGRRVLLIDDVVTTGATVTACAQALRAAGAGHV
                     TALSLLRVVRE"
     gene            1278..1787
                     /locus_tag="PKL17_11060"
     CDS             1278..1787
                     /locus_tag="PKL17_11060"
                     /inference="COORDINATES: protein motif:HMM:NF018499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HNK45316.1"
                     /translation="MEAEAKHPAVLRDIIVDDVTWRCATRPRRAEWQTAIDELISSAA
                     FALEGETQKSPERALHGQVSVEPGSIAMTLRDEAGAEVGRLLLERPVIGPVLREYLRI
                     LRAIESSSLSETSPHFEALDIARRLIHDDAAQLVQRYFSGAHPDQETARQLFSLLVLL
                     THDTSRLAS"
     gene            complement(1812..3836)
                     /locus_tag="PKL17_11065"
     CDS             complement(1812..3836)
                     /locus_tag="PKL17_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNK45317.1"
                     /translation="MTQKTARSETPSTSETSRWLHVRLWATGTLLTACFGVLIVRAYV
                     LQVQKRDLYRSLAEEQYLREIEVPPHRGRIVDRSDSELAASASVDSVYIEPQKFRSEL
                     PDVARRDAALGRLAMALSLEPQELRRRALSDRHYLFLKRRIPPEEAQKVRSLALPGIG
                     LTQEPKRYYPNRSLAGPLLGWAGVDAIGLEGVELAYDRFLRGSKASVIGWQDARGRKV
                     MVGGVGDSAKERGHDVHLTLDKFIQFRLEQALEDGVHRARAKAGVAVAIDPRNGEVLA
                     MASVPSVNPNDPAGARERGVRNRVVTDPFEPGSTIKPFTIGAAIEAGAIHLTDEWDCE
                     GGQWRLGHVTIHDADPEGILTTTQVLARSSNICTSKITRRIGKEKMYLFLRQLGFFAP
                     SGIDLPGERSGQVPSVGEWGDIAFANISFGQGMTATPIQIASAMAAIANGGILYRPHV
                     VSKVVSATGQSVLESHPEGKRVMSPRTASLLRQMLRAVMEKKGTGDKLDIPGYPVAGK
                     TGTAQKVDPNTRRYSPEYWASSFVGFAPYDDPRILLYVMVDEPREGHYGGEVAGPIFV
                     KVASAVLPYLGVPPQNSPTVVTTTPPSRALLTEPYGPPVPVAIRYPGVPDAQRVPSFV
                     GQGLGRAVELAAKAGLRLSVRGSGVVVSQEPPAGSARRGSVIQLRLAPPH"
     gene            complement(3833..4222)
                     /locus_tag="PKL17_11070"
     CDS             complement(3833..4222)
                     /locus_tag="PKL17_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNK45318.1"
                     /translation="MAAVSSRRKVSFWRRQGEKIHRVITWPSQSPSFGLTVGLAVSSL
                     VLIALLYVWSRLEVIRIGYELSQQSKLARALAQHNQRLRLELATRKDPATIERFARER
                     LHMAPPLPSAIRIVKSTKGRSAEGSSR"
     gene            complement(4210..5205)
                     /gene="rsmH"
                     /locus_tag="PKL17_11075"
     CDS             complement(4210..5205)
                     /gene="rsmH"
                     /locus_tag="PKL17_11075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNK45319.1"
                     /translation="MEAWANLHQPVMADEVLRLLAVRPGGVYCDATLGLGGHSERILS
                     ALDGEGTLFGIDRDETALALATDRLARFGPRFRPLHGRFGDLAELLAAAHAPPIDGLI
                     ADFGVSSLQLDRPERGFSFQNAGPIDMRMDTSQGETALELLQRIDEPALADILWEYGE
                     ERHSRRVARALKQAVGDGKLTDTLSLATVVAKAIPQREPHKHPATRTFQALRMAVNDE
                     LRQIHSLLLAAPRLLAPGGRLVVISFHSLEDRLVKQHLSFVPAVASAHQSRGMPRVEN
                     PFILLSKGTLTPTEAEQSANPRSRSARIRAARTWTVEERQKQDRSPRPEGAPWPL"
     gene            5472..6428
                     /locus_tag="PKL17_11080"
     CDS             5472..6428
                     /locus_tag="PKL17_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45320.1"
                     /translation="MRSMRPLKGFLSFVTPLLGAIALLSAEGCTEQNKSRFYTYCDAT
                     GCFQCDVAGCVAQDGRPPGSACVSSSDCAPGCYCGSKGQCAEAGYCEKQADCASGFVC
                     NSARHSCEPDAAATTPKSCKVQSDCGSGSECNNGSCKTAPVPPNHCVFNRECGAGGAC
                     VDGQCQKACSADTGCGTGQACVNQRCQAKPVDKTSCVTSAQCGAGQACIDSACHPSCS
                     KDADCQAINKNDLCIASVCRADTRRVPECKANADCGAGRECVNAQCRTFCMANSDCAS
                     CNDGKVCSTGYCMTDREVSPQCKLAADCKSTALHCTDGACGQ"
     gene            6492..7361
                     /locus_tag="PKL17_11085"
     CDS             6492..7361
                     /locus_tag="PKL17_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNK45321.1"
                     /translation="MPASVIAFLEWLVRSAWGTVFAVLWGAMWGSFANVCIHRLPLGQ
                     SVVRPGSRCPSCSQPIAWFDNIPILSFLLLRGRCRHCAAAISGRYPLIEMLAALLCFL
                     TFWRFVQHAGTDEPLALQLARCVVYGPFLLLLLVLSAIDLEHQLLPDRLTLPAIGLFF
                     GAGRILGDVSLGDSLLGIVVGYGLVWLISEAYYRMTGRDGLGLGDGKLLALVGATLGA
                     RALPWTLLVGSVLGTLVALPVLLWRRRREPTTSIRHVAIPFGPFLSLAAAIYLLLLLG
                     KSLDEILAYVPVY"
     gene            complement(7373..8776)
                     /locus_tag="PKL17_11090"
     CDS             complement(7373..8776)
                     /locus_tag="PKL17_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNK45322.1"
                     /translation="MSHVENHSLEKQAELRALAERVTELAIDDGACAAEVIVGAGQSL
                     SVKVRRGQKELLHESESHGLGLRVFVNHRSAVCHTSDLSESALRRFVHETVELSALSE
                     PDELNELPHRDELVQPGQKLPQLALWDERTAQLSAPELFDLARRCEAAALAHSQKITN
                     TDGASADRNAGYMALALADKSGLAFSGSSRGTYCSFSVEALCDDDSGKKRNASYWSAN
                     RFFARLDSPESVGAEAARRALSKLGAQKLATAELPVVFDPDASRALLRTLSSVVSGGS
                     IYRKSSYLCGRENTEVASPLITVVDDPLLPNGPGSRVFDGEGLLARRNVIVEKGILRG
                     YLLDNYSARKLGRKSNGCAGRSVGGSPHVTTSNLILCAGSTAPDQLLCGIERGLLVTD
                     MMGFGFNAVTGDFSRGASGFLIEKGQITVPVSEVTISANFDELLKRIDAVGSDIDERS
                     STMSPSLRVTRMTVAGH"
     gene            complement(8798..10228)
                     /gene="tldD"
                     /locus_tag="PKL17_11095"
     CDS             complement(8798..10228)
                     /gene="tldD"
                     /locus_tag="PKL17_11095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNK45323.1"
                     /translation="MPVSPRPIESAFTGLSDEELRRLLSLALSKGGDYADLYFEHCQT
                     ASYVFEDERVKTVGRGVSLGLGVRVQRGEATGYAYCEGLDETAMARAARTAAEIAESA
                     ARPGPQQIESRLTPSFYRIDVPSLQAPAKDKLDLLRRGDRAARAADRRIVKVVCSFSE
                     EQREILIVTGDGRRYSDQQPLLRYGIHVVAEDGKKRQSGSGGGGGRYAFDFFAKKTPE
                     HYAGEATHVALAMLEAVEAPAGQLPVVLAAGDSGILLHEAVGHGLEADFNRKQTSNYS
                     GRIGEVVASELCTVVDDGTVENSRGAINVDDEGIPGQRNVLIEGGRLRAYMQDHLSAR
                     IMGTSPSGNGRRQSFRHEPMPRMTNTYLLGGQDSPEDIIRSVRRGVFARRFSGGQVNI
                     SNGDFVFSLTESYLIEDGKLTAPLKGVNLIGNGPDVLTKVSMVGHDFEMSDGIWTCGK
                     EGQSVPVGIGTPTVKIAAITVGGTKT"
     gene            complement(10303..11280)
                     /locus_tag="PKL17_11100"
     CDS             complement(10303..11280)
                     /locus_tag="PKL17_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45324.1"
                     /translation="MGAKFSSELAAQGGVVSLKLRGVVDEDNELAGIEGQLSAGITVL
                     DLADIERINSCGVRDWVNWLGRIEKIGAKLVFVNCSPAIVAQMNLVHNFSASGIVKSF
                     YAPYFCPRCNKERLLRLETKDLVHQTPITSAPTCRCDDCDGPMDFDDMEESYFAFLNN
                     LKKVMSEPATEEILQTVAAASEGGAKQKTRGSSAGMTPATGPGNTPATPSTGAPSSMS
                     PVPKTPTGRSPSGALPMPKTPPGNALSGLVSQQGEAPATSSGNHRPVGNAAAQATTPT
                     ALGSPDKAPHSSPAMGQTQPSMAKTIVMWFGLAVFLIAAIGAMIYAIAK"
     gene            complement(11363..18007)
                     /locus_tag="PKL17_11105"
     CDS             complement(11363..18007)
                     /locus_tag="PKL17_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45325.1"
                     /translation="MSESAAPSAEPSGTLPAESARSPSPEARLQALVGQAAQPVLDDL
                     LQLLLAELGETQDPTEHAELAVRMAMLLWDGQGKADAALALLGTAHHPVASMLRLQAA
                     LEGRAQGPQLADYTDWVIRHGSADEKSELGEILLWQGQLDLAETLLAEGSAPRIYALL
                     LTSQALTVRAADTLSSSVDGNDLLWAASLRVQAGQGSSDPSVVMLSVQQLAQQAERTP
                     KRSDLAGMVLALRASEHHLASLPAYGPGQWQLLNERFALLGGDPVLRGASPPVELLVT
                     ALELATASMNSQVMATQAADLLVSISQVTSIHPTLTAQLSQHLIERRAAQQLERVGRF
                     GEAAQYWQHQANRLIEGLPAPAAESSSDEWNRALAMAHLYRAVECWILAIPQQGDEAR
                     SQAVQQATRLLSFGIVDEVLMLRIATQLLQGNDRRSVCEWLLACAQACPPASPERAIL
                     VAHAARAAEALQATEREGLLSLPQLADRPALQTLARQFRRRSDVARLLGVYRRLADSV
                     SAGDTDGQTLAATYASVAAILAAVLLPDPLPEAVAGETELPRLLSSLLAQAEPFADAA
                     GGLLISIARVLTCQRRNHDGELVEALVELLNRVQSPRSKLRVLKQLAHHATDAGDLQR
                     AEVCYQEVLERDPTDVVAMHALARLLQQRGDTKQAIELLRTAVEAALTIGGEPSAALP
                     STMAMLSPRKLGLGDTSAPVGAQAAALLACELGALFEQKASQGGDTAALSEALTAYEG
                     ALRRDPRCRPAARALVSLYRTLGQKTEQLTAMRRLLPLLRDDHQRFELLIELGQLAEH
                     QGKLIGGEASAPLIEQAIDAYSEALQLSPAHEGAIASLVSLCLVDGRYGLIAETLERA
                     PKTPYLLRVLGEAYEKLGQPDGLARTLEELLPQLAGPDEQRQVLTQLAELYARLERPA
                     DEIRIWERLYELSPEMILGSSKMLLQLELRYGASGRHAQQAQLLGHALSRIDKPKSKL
                     SVDEADLKRQLLLRLGQVQREFLSQNAEAVRTFEKVLSEWPEERTALASLVTLYTQTE
                     QYADLRRTLTTLLRQVQEPSERSRYLFALGELLDKLGEADDAYRLNGEAFFLDPANRA
                     AFTAYERACYRRQQWTETLKIYDAALRLIETQKTRSYRPADLYLRRGQVQLQYLQQVD
                     EAIGSYLKALESDAENDTTQATLERVYAGRNQWKELLAAYERRAQLVRDDSKRVEILR
                     RAARVATAKLRDVIEAVRFYEKLHAVDPTDTESLDALEHHYESARDYEKLVALLSTRV
                     TLASEEQQIIALHMRIGLLCEEGLRDHDRAISAYRHVVEAQPTHREALDAMARLLEAN
                     ERWAELVEVTRRQIRLVTDRQHKALLYFKCGSVTEAKFGKEDEAIRYYEAAVRTSAAC
                     LPALHSLRDIYVRREDYARVTQTLELESKLWTEPKEQAGILAHIGQIYLDKLHQPERA
                     IEYYERALTVDKDCLPAHRALFHVYYQRGDFYRTYQTGQVLLVRAAREGEPSERSTFH
                     TKRAHAASKVGELRVACESVGLALEIWPENLAALHLLVSLCLLPETGYDFAPTCRELE
                     KQYRRRNVPEPLALLLCAQAALAERSGDSDGAEALLGEALKLQPDESQVVELVAAAYV
                     RLARWDAAVTLLQSFIERLRQAGNAAQLATVARCELILAGLFAEVLLQPEAAIKLLRQ
                     SIEAATRPEASVPEELLHRARFVLSQELFRIGRYLEARTEIDELLRSVQPDGTPKVTP
                     SVLCRYSDYQGRILESLGDMTGAQQAYLKAIDLDPTYAPPVLQLSRWGVLTGNRDQAE
                     ALLRDAIERSGERTVAAGYDERASEIFLRRGLARLLVASEPQAAVEEYRRLIDLSADD
                     YIPLPLCFDLASPGGPPLLWNGQDDRVSLAEVLLQNIGDTSQSQQVLGQILSEDLRCA
                     AAYPLLAEVYERRNETIRARRIRTVSALLGYSPPEKRPLRSGGYRSTLSEELRQRLLL
                     PPLLRGSLTVDLLQAMVPGLLRLFPPTWPWPNEPVPATKVPDAGFQSAVSEVLRLFAI
                     DAEVLVAHQVPGGAQAMELPTGRKVALIDQSALAMGDSERRFVLGRTLDALRGGYATV
                     LRLSPSELLQTSRLLTMMLLPTAEQPPESREFLQLLPRRSQQVFERAAQSRPTQTVAE
                     VVASFTLCADRAGLLACDDIATAVQMLVKLTGDDAASHGLSDVAGTIGMVLGHAPNGA
                     ELLHYYLSDTFHELTQTLREPSRL"
     gene            complement(18099..>18637)
                     /locus_tag="PKL17_11110"
     CDS             complement(18099..>18637)
                     /locus_tag="PKL17_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45326.1"
                     /translation="HAVLLFDEADSLFAKRTEVKTSNDRYGNLAVNYLLQRLEQYTGV
                     VVMTTNKEASLDEALQRRLTLHLHLEIPEPPERERLWKSMLPQNAPWAKDINWKALAK
                     DYEISGGYIKNAVLRAAFLAADANTPINMTLLRRSASLEMEDMGRLIARGGRAGVSGV
                     PSLISAARRENSDGPGPA"
BASE COUNT         3372 a         5709 c         5821 g         3735 t
ORIGIN      
        1 gattgggctt tatggcggac aagcgcaagg cgcggtcggt ttttcgtttg gtgtcgagcg
       61 cctgatcgat ctgctcaaag agcaggtggc tggccagctt cagacagcgc gacctgtgct
      121 ggtggcgccg cttggtgttg gtcgaattgc cgatgccgag cgggtggcga tgacgctacg
      181 tcaggcggga attgcaacgc gactttccct ttcagcgacg gctcccggta agcatcttca
      241 gtatgccgaa tcggtgagca tcgatcatgt gatcttcgtc ggtgaagacg aagtgaaaca
      301 gggtctatat ccgtgcaagc agctttcgac gcgaagtgaa gcgcgtctgc cgatcgatgg
      361 tctttcgggg catctgcgcg ctgccggtgt gctcactctc tgacgacgcg cagcagtgac
      421 agggcggtta catggcctgc gcctgctgcg cgcagcgcct gagcacaggc tgtgacggtg
      481 gccccggtcg ttacgacgtc atcgataagt agaacccgtc tgccgcgaac ttttgcctcg
      541 gctgctttgg ggacgccaaa tcgactgcgg acacgcgcca agcgggcagc gcgacccagg
      601 tggtgtgctg ctgttgtcgc tcgggtggcg acgagcagac caggatgcag gcgggcggca
      661 ggcggggcca cggcgcgcag tgcttcttgg gccagcagcg ttgactggtt gtagccacgt
      721 tcgcgcagcc gactgcgatg cagcggaacc ggaacaatcc agtcgtgctg ggtcgcaagc
      781 tcggcaagga gcggggtcaa gagtcggccc agcggcagcg cgagatcgtc gcggccttgc
      841 cacttgagca gcagcagggc atgggacagc ggcccatcgt aggcaaaggc cgaacgaagc
      901 ggcggcgcgt ctggaggcag atcggtcggc tcatcgggat tggcttcaca gcgacagaaa
      961 cagtcgaaac aaagtggacc ctgacgatat ccctccgcga gaggctcttc acaggcggcg
     1021 cactcgtgtc ggcccagcag aagccacagc gcattccaga tctcccaaaa cggtcgccac
     1081 atcgacatct cattcggtat cggcagactg cccggacaag ttgcgcagtt tggttctcca
     1141 tttttttacg tctgacgctt tgacgcgagc ggtgacgcgg acacttgggc gcgtcgtcca
     1201 gacgggttag aaggcacgtt tgacgcagtg cagcaaagtg attgtcaagc tggccggacc
     1261 gcgattacag ttttggtatg gaagcggaag ccaagcaccc agcggttctt cgcgacatca
     1321 tcgtggacga tgtgacctgg cgctgtgcga cgaggccacg acgggccgag tggcagactg
     1381 cgatcgatga gctgatctcc agtgcggcct ttgcgcttga aggcgagacg caaaagagtc
     1441 ccgagcgagc acttcatggc caggtttccg tcgagcccgg ctcgattgcg atgacgctgc
     1501 gggatgaagc gggcgcggaa gtgggcaggc tgctcctcga aagaccagtg attggtccgg
     1561 tgctgcgtga atatctgcgc attctgcgcg cgatcgagag cagcagcctg tccgagactt
     1621 cgccgcattt tgaagcgctc gacattgcgc ggcggctgat tcacgacgac gcggcgcagc
     1681 tcgtgcagcg ctacttttcg ggggcgcatc ccgatcaaga aacggcgcgg cagttgtttt
     1741 cgctgttggt gctgctcacg cacgacacca gtcggttggc gtcgtagcac agcctgtccg
     1801 tcgggcccga atcagtgggg cggtgcaagt cggagctgaa tgacgctgcc tcgacgagcg
     1861 ctgccagcgg ggggttcttg cgaaacgacg accccgctgc cgcgcacgga cagccttagc
     1921 cctgctttgg cggccagctc gacggcgcga ccgagtccct gtccgacgaa gcttgggacg
     1981 cgctgggcat cggggacgcc gggatagcgg atcgcgaccg gcaccggggg accatacggt
     2041 tctgtaagaa gcgcccgtga cggtggcgtt gtcgtcacca ccgtcggtga attctgaggc
     2101 ggaacgccca aatagggcag cactgccgac gcaaccttga cgaagatcgg tccagctacc
     2161 tcgccgccgt agtgtccttc ccgcggttcg tcgaccatca catataaaag gatgcgcggg
     2221 tcatcatacg gggcaaatcc gacgaaagac gacgcccaat actcgggtga atagcgccgc
     2281 gtattcggat cgactttctg cgctgtgcca gtcttgccag cgacgggata ccctgggatg
     2341 tcgagcttgt cgcccgtgcc ctttttttcc atcacggcgc gcagcatctg tcgcaaaagc
     2401 gaagcggtgc gcggtgacat cacgcgcttt ccttcgggat gcgattcgag caccgattgg
     2461 cccgtcgcag acaccacttt gctgaccaca tgcggtcggt agagaatgcc gccgttggcg
     2521 atcgccgcca ttgccgaagc gatctggatt ggcgtcgctg tcatgccttg accaaagctg
     2581 atgttggcaa atgcgatatc gccccattct ccgacggatg gaacttggcc gctgcgctct
     2641 ccgggaagat cgattccgct cggtgcgaaa aaaccaagct gacggagaaa caggtacatc
     2701 ttctctttgc cgatgcgccg cgtgatcttg cttgtgcaga tgttcgacga gcgagcaagc
     2761 acttgcgtcg tcgtcaaaat gccttcgggg tcggcgtcgt ggatggtgac gtgacccaat
     2821 cgccactgac cgccttcgca gtcccactcg tcggtcaggt gaatggctcc cgcttcgatg
     2881 gctgctccga tggtgaatgg cttgatcgtc gatcctggct cgaatggatc ggtgacgacg
     2941 cggttgcgga cgccgcgctc acgagccccc gccggatcgt tcggatttac cgatggaacc
     3001 gatgccatcg ccagaacttc gccgttgcgt ggatcaatgg cgacggcaac accggccttg
     3061 gcgcgcgctc tgtgcacgcc gtcttcaagc gcctgttcga gtcgaaactg gatgaacttg
     3121 tcgagcgtca ggtggacatc gtgaccgcgt tctttcgccg aatcaccgac gccaccaacc
     3181 atgactttgc gaccacgggc gtcctgccag ccaatcaccg acgctttgct gccgcgcaaa
     3241 aaacgatcgt aggccagctc gacgccctca aggccaatcg cgtccacgcc cgcccagccg
     3301 agcagcggtc ccgccaagct gcggttcgga tagtagcgct tcggttcttg cgtcaggcca
     3361 atgccgggca gcgcgagcga ccgtactttc tgggcttctt ccggcggaat ccgtcgcttc
     3421 aggaacagat agtgccgatc agacagggct cgtcgtcgca gttcttgggg ttcgagcgaa
     3481 agtgccatcg ccagccgacc gagcgccgcg tcacgacgtg cgacatcggg tagctcgctg
     3541 cgaaactttt gtggctcgat gtagaccgaa tcgacacttg ccgatgcggc cagctcgctg
     3601 tcactgcggt cgacgatgcg gcctcggtgc ggcggtactt caatttcgcg caggtactgt
     3661 tcttcggcga gcgagcgata cagatcgcgt ttttgcacct ggagcacgta ggcgcgcaca
     3721 atcagcacac caaagcaggc cgtcagaagc gttcccgtcg cccacaatcg aacatgcagc
     3781 cagcgcgagg tttcgctggt gctcggagtt tcgctgcgcg cggtcttttg cgtcatcgcg
     3841 aggaaccttc cgcactgcgg cctttggtcg atttgacaat ccgaatcgcc gacggcaaag
     3901 gcggtgccat gtgcagtcgt tcgcgcgcga aacgttcgat cgtcgcagga tctttgcgcg
     3961 tcgccagttc cagcctcagc cgttggttgt gctgggccag cgcccgcgcc agcttgcttt
     4021 gctgcgacag ttcgtaacca atgcggatca cttcgagtcg agaccagacg tagagcaggg
     4081 cgatgagcac cagcgacgac accgcaagac caacggtcag tccgaagctt gggctctggc
     4141 tcggccatgt gatcacgcgg tgaatttttt caccctgccg tcgccaaaag ctgacttttc
     4201 gacgcgaact tacagcggcc atggtgcccc ctcgggccgt ggcgaccgat cctgtttttg
     4261 gcgttcttcg acagtccagg tacgcgcagc gcgaatccgt gccgagcggg accgcggatt
     4321 cgccgattgc tcggcttccg tcggtgtcaa cgtccccttg ctgagcagga tgaacggatt
     4381 ttcgacccgt ggcatgccgc ggctttgatg ggcagacgcg acggcaggga cgaatgagag
     4441 gtgctgcttg accagccgat cttccagcga gtgaaacgaa atgacgacga gccgtccgcc
     4501 cggcgccagc agccgcggtg ccgccagcag cagcgaatgg atctgacgca gttcatcatt
     4561 aacggccatg cgcagcgcct gaaacgtgcg cgtggcggga tgtttgtgcg gctccctttg
     4621 cggaatggct ttggcgacga ctgtggccag gctcagtgtg tccgtcagct ttccgtcgcc
     4681 caccgcttgc ttgagcgctc gcgcgacgcg acggctgtga cgctcttcgc cgtactccca
     4741 caagatgtca gcgagcgctg gctcgtcgat tcgctgcaac aattccagcg ccgtttcccc
     4801 ctgcgaggtg tccattcgca tgtcgatcgg gccggcgttc tggaacgaaa agcctcgctc
     4861 ggggcgatca agctgaagcg acgagacgcc aaaatcggca atcagtccat caatcggtgg
     4921 tgcgtgcgcc gcggccaaaa gttccgccag atcgccaaag cgtccgtgca gtggtcgaaa
     4981 tcgtggccca aaacgagcca gtcgatccgt cgccaacgcc agcgcggttt cgtcacgatc
     5041 gatgccaaac agtgtgcctt caccgtcgag agccgagagg atgcgctcgc tgtggccgcc
     5101 cagtccaagt gtcgcgtcgc agtagacgcc accgggtcgc accgccaaga gccgcagtac
     5161 ctcatccgcc atgaccggct ggtggagatt cgcccaggct tccatagtca gcccgacatc
     5221 ccaacagcca ggccgggggc cgtcaaattt tcggcgatgc agcgtgacct gcgaaccagc
     5281 gaacacactg cgtctgtgac cagttgttca cagcatgcgg gccggacaga acggagtgcg
     5341 cagtctccaa cgaaaatcag cgactctccg cgcctgtggg gtcaaagatg gctggcatac
     5401 tggctgctgt gccctaaagc atggtaaatg tgtttggtaa gttcatctcg acgaatcgtg
     5461 gagggcttcc gatgcgctct atgcgtccgc taaaagggtt tctgtcgttt gtaactccgc
     5521 tccttggtgc gatcgcgctg ctctctgcgg agggctgcac cgagcagaac aagtctcgct
     5581 tttacaccta ctgcgacgcg acgggatgct tccagtgcga cgtcgctggc tgtgttgcgc
     5641 aggacggacg cccgccggga agcgcctgtg tttcgtcgag cgactgcgcc cctggctgct
     5701 actgcggcag caagggccag tgcgccgaag ctggttactg cgaaaaacag gctgactgcg
     5761 ccagtggttt cgtctgtaac tcggctcgtc actcgtgcga gccggacgct gcggccacaa
     5821 cccccaagag ctgcaaagtt cagtcggact gtggatcggg cagcgagtgc aacaacggct
     5881 cctgcaagac cgcgccggtt ccaccgaacc actgcgtgtt taaccgcgag tgtggcgctg
     5941 gcggcgcctg cgtcgatgga cagtgccaga aagcttgcag tgccgacacc ggctgcggaa
     6001 ccggccaagc gtgtgtaaac cagcgctgcc aggcgaagcc agttgacaag acttcgtgcg
     6061 tgaccagcgc gcagtgcggc gcgggccagg cctgcatcga cagcgcttgc cacccgtcgt
     6121 gcagcaaaga cgccgactgc caggccatca acaaaaacga cctgtgcatc gccagcgtgt
     6181 gccgcgccga tacccgtcgc gtgcctgagt gcaaagccaa cgccgactgc ggagccggtc
     6241 gtgagtgtgt gaacgctcag tgccgtacct tctgcatggc caacagcgac tgcgcgagct
     6301 gcaacgacgg caaagtttgc agcaccggct actgcatgac ggatcgcgaa gtcagcccgc
     6361 agtgcaagct cgccgccgat tgcaagagca cggcgctcca ctgcaccgac ggcgcctgcg
     6421 gccagtaagc tctctccttc ggcttccttc tcttttggtc ccggtcttgg ggacggcgct
     6481 ttttcttcat catgcccgct tcagtcatcg ctttccttga gtggctcgtc cgctctgcct
     6541 ggggcactgt cttcgcggtg ttgtggggcg cgatgtgggg cagcttcgcc aacgtctgca
     6601 tccatcggct gccgctcggt caatcggtgg ttcgccccgg ttcgcgctgt ccgtcgtgct
     6661 cgcagccgat cgcgtggttc gacaacattc ccatcctcag ctttcttttg ttgcgagggc
     6721 gctgtcgtca ctgtgctgcg gcgatttctg ggcgatatcc gctgatcgaa atgctcgcgg
     6781 cactgctttg ctttctgacg ttctggcgct ttgtgcagca tgctgggacg gatgaaccgc
     6841 tcgcgcttca gcttgcgcgc tgcgtggtgt atggcccctt tttgctgctg ctgctcgtgc
     6901 tgtcggcgat tgacctagaa catcagcttt tgccagatcg actcacgctg ccggcgattg
     6961 ggttgttttt tggcgcgggg cgaattcttg gcgacgtaag tctcggtgat tcgctgctgg
     7021 gcatcgttgt gggctacggg cttgtgtggc tgatttccga agcctactac cggatgacgg
     7081 ggcgagacgg gctcggtctg ggggacggca agcttctggc gctggtgggg gcgacgctcg
     7141 gcgcgagggc tttgccgtgg acactacttg tcggttcggt gcttggaacg cttgtcgcgt
     7201 tgccggtttt gctgtggcgt cgtcgacggg aaccgaccac ttccattcgc catgtggcga
     7261 ttccgtttgg tccgttcctc tcgctggcgg cggcgatcta tctgctgctg cttttgggca
     7321 agagtctcga cgagattttg gcctatgtgc cggtgtacta gcgtcccggt tgtcagtgtc
     7381 cagcgacggt catgcgtgta acccgcagac tcgggctcat cgtcgatgag cgttcgtcga
     7441 tgtcgctgcc cacggcgtca atgcgcttga gcagctcgtc gaagttggcc gaaatggtca
     7501 cttcgctgac gggaaccgtg atctggcctt tttcgatcaa gaagcccgat gcgccgcgcg
     7561 aaaagtcgcc agtgacggcg ttgaaaccga agcccatcat gtcagtgacc agcagaccgc
     7621 gctcgatgcc acaaaggagc tggtccgggg cggtgctgcc cgcgcacagg atcaggttgc
     7681 tggtggtgac atgcggcgat ccaccgacgg aacgtcccgc acagccgttg cttttgcgac
     7741 ccagcttgcg ggcggaatag ttgtcgagga gatagccgcg caagattccc ttttcgacaa
     7801 ttacgttgcg ccttgccaaa agtccttccc catcgaatac gcgcgatccg ggcccattgg
     7861 gcagcagcgg atcatcgacg acggtgatga gcggcgacgc aacctcggtg ttttcgcgac
     7921 cacagagata agagcttttg cgatagatgg agccgcccga aaccaccgac gacagcgtgc
     7981 ggagcagggc gcggctggca tccggatcga agaccacggg cagctccgca gtggcaagct
     8041 tctgcgcacc gagcttgctt agagcccgac gggctgcttc ggctccaacg gactcggggg
     8101 aatcgagacg ggcgaaaaag cggtttgccg accagtacga agcattgcgc tttttgccgc
     8161 tgtcatcgtc gcagagcgct tcgacgctaa acgaacagta ggtgccacgc gacgatcccg
     8221 aaaaagccag gccggatttg tcggccagcg cgagcgccat gtatcccgcg ttgcgatcgg
     8281 cgctggctcc gtctgtgttg gtgatttttt gcgaatgcgc cagcgcggcg gcttcacagc
     8341 gtcgggccag atcaaaaagt tcaggagcgc tgagctgcgc ggtccgctcg tcccaaagcg
     8401 caagctgggg cagcttttga ccgggctgga ccagctcgtc gcgatgcggt agttcgttta
     8461 gctcgtcggg ttcactcagc gcactcagct cgacggtttc gtggacgaag cgacgcaggg
     8521 cggactcgct caggtccgac gtgtggcaga ccgccgagcg atggttcaca aacacgcgca
     8581 gtccgagccc gtggctttcc gattcgtgca gcagctcttt ttggccgcga cgtactttga
     8641 cgctgagcga ttgtccggca ccgacgatga cttcagcggc acaggcacca tcatcaatgg
     8701 ccagctcggt gacgcgctcg gccaaggccc gaagctcggc ttgcttttca agcgaatggt
     8761 tttcgacgtg tgacacgagg ttctccgcgg cgctgcgcta ggttttggtt ccaccgacag
     8821 tgatggcggc gattttgaca gttggtgtac cgataccgac ggggaccgac tgtccttcct
     8881 tgccgcaggt ccaaatgccg tcggacatct cgaagtcgtg acccaccatc gaaactttgg
     8941 tcaggacgtc ggggccattg ccgatgaggt tgacgccttt gagcggtgcc gtcagcttgc
     9001 catcctcgat gagatagctt tcggtgagag agaagacaaa atcgccgttc gagatgttga
     9061 cctgaccccc cgaaaaacgg cgcgcgaaca cgccgcgacg gaccgatcga atgatgtctt
     9121 cgggcgagtc ttggccaccg aggagatagg tgttggtcat gcgcggcatc ggttcgtgac
     9181 ggaagctctg acggcgtccg ttcccgctcg ggcttgtgcc catgatgcgg gccgacaagt
     9241 gatcctgcat gtacgcgcgc agtcggccac cctcgataag gacgttgcgc tgtccgggga
     9301 ttccttcgtc gtcgacgttg atggcgccgc ggctgttttc cacggtgcca tcatcgacga
     9361 cggtgcacag ctctgatgcg acgacttcgc cgatccgacc cgaataattc gaggtctgct
     9421 tgcggttaaa gtccgcttcg agaccgtgtc caacggcttc gtgcagcaaa atgcccgagt
     9481 cacctgcggc caatacgacg ggcagctgac ctgccggggc ttcgaccgct tcgagcatgg
     9541 cgagcgcgac gtgggtggct tccccggcat agtgctcggg ggtttttttg gcaaagaagt
     9601 cgaaggcgta gcgtccgccg ccgcccccgc tgcccgactg tcgctttttg ccgtcttcgg
     9661 cgacgacatg gatgccataa cgcagaagtg gctgctgatc ggaatagcgg cgaccgtcgc
     9721 cagtgacaat caatatctcg cgctgttctt cggaaaagct gcagaccact ttgacgatgc
     9781 gacggtcggc tgcacgggct gcacgatctc cccgacgcaa caaatcgagc ttgtctttgg
     9841 ccggcgcctg caggctgggc acgtcgatgc gataaaacga cggcgtcagc cgagactcga
     9901 tctgctgcgg gcctgggcgg gcggcggatt cagcgatttc ggcggcggtg cgggctgctc
     9961 gagccatcgc tgtttcgtcg aggccttcgc agtaggcgta acctgtcgcc tcgccacgct
    10021 ggacgcgcac gccgagcccc aaagagacgc cgcgaccgac cgtttttacg cgctcgtcct
    10081 cgaagacgta gcttgccgtt tggcagtgct cgaaatacag gtcggcataa tcaccgcctt
    10141 tgctgagcgc caagctgagc agtcgacgca gctcttcgtc ggacaaacca gtgaaggcag
    10201 actcaatcgg gcggggagag actggcaagc cactttcgtg catgggccca aagtgttgcc
    10261 caaaggccca tcgagcgcaa tggatggcga agacgaggga gcttactttg cgatcgcgta
    10321 gatcatggcg ccgatggcgg caatcaagaa cactgcgagg ccaaaccaca tcacgatggt
    10381 cttggccatc gacggttgtg tctgacccat cgccggggaa gagtgcgggg ctttgtctgg
    10441 gctaccaagc gcagtcggtg tcgtcgcctg ggctgcggca tttccaaccg gacggtggtt
    10501 gccgctcgac gtcgctgggg cttcaccctg ctgcgacacc aggccagaaa gcgcatttcc
    10561 cggtggcgtc tttggcattg gcagcgcgcc gcttggagat cgtcccgtcg gtgtcttcgg
    10621 tacaggcgac atcgatgacg gtgcgcccgt cgatggggtc gctggcgtgt tgcctggtcc
    10681 cgtcgctggc gtcattccgg cactgctgcc gcgggtcttt tgtttggcgc cgccttcgct
    10741 ggctgcggcc acggtctgga ggatttcttc cgtcgctggc tcgctcatca ctttcttgag
    10801 gttgttcaag aaagcgaagt acgattcttc catgtcgtcg aaatccatcg gcccgtcgca
    10861 gtcatcgcag cggcaggtcg gggccgacgt aatcggcgtc tgatggacca gatccttggt
    10921 ttccagtcgc agcaggcgtt ctttgttgca gcgcgggcag aagtacggcg cgtagaagct
    10981 cttgacgatg ccgctcgccg agaagttgtg gacaagattc atctgcgcga cgatggccgg
    11041 cgagcagttt acaaacacca gcttggcacc gatcttttcg atccgtccga gccagttgac
    11101 ccagtcgcgc acaccgcacg agttgatgcg ctcgatgtcg gccaaatcta ggacggtgat
    11161 cccggctgag agctggcctt caatgcccgc aagctcgttg tcttcgtcga caacgccgcg
    11221 aagcttgagg cttactaccc cgccttgagc cgctagctca gagctgaact tggcacccat
    11281 gttcccgtcc gccctctttc ttgcgtaacc accgccgcta ccgccatatc aaggcccaag
    11341 aatacctttt gctgggggca gatcacaagc gcgatggttc tcgtagcgtc tgagtaagtt
    11401 cgtggaaggt gtccgaaaga tagtagtgca aaagctcggc accatttggt gcgtggccta
    11461 agaccatgcc gattgtgccg gccacgtcgg acagtccatg agatgccgca tcatcgccgg
    11521 tcagcttcac cagcatctgc accgccgtcg caatgtcgtc gcaggcaagc agaccggcgc
    11581 gatcggcgca cagcgtgaag ctggcaacca cttcggccac ggtctgggtc ggtcggctct
    11641 gcgcggcgcg ttcaaacacc tgctgcgatc gacgcggcaa aagctggaga aactcgcggc
    11701 tttcgggagg ctgctcggcg gtcggcagca gcatcatcgt cagcaggcgc gaggtctgca
    11761 gaagttcact cggcgaaagc cggagcaccg tcgcatagcc gccgcgcagt gcatccaggg
    11821 tccgtccgag gacaaatcgt cgttcgctgt cgcccatcgc gagcgctgat tgatcaatca
    11881 gcgcgacctt gcgccccgtc ggcaattcca tggcttgtgc gccgcccgga acttggtgcg
    11941 caaccagcac ttctgcatcg atggcaaaca gccgcagcac ttcgctgacg gcgctttgga
    12001 aaccggcgtc gggcactttc gtggctggca caggttcgtt tggccacggc caggtcggtg
    12061 gaaacagtcg caaaagtccc ggcaccatcg cttgtagcaa atcgacggtg agcgatccac
    12121 gcagcaaagg tggcagcagc agccgttgac gcagttcctc gctcagcgtg ctcctgtacc
    12181 cgccactgcg cagcggtcgt ttttccggcg gcgagtagcc aagcagcgcg gaaaccgtgc
    12241 ggatgcgacg ggcccggatg gtttcattgc gtctttcgta gacttcggcc aacagcggat
    12301 aggccgccgc gcagcgcagg tcctcactga gaatttgacc cagcacttgc tgcgactgac
    12361 tggtgtcgcc gatgttttgc agaagcactt ccgccagcga cacgcgatcg tcttggccat
    12421 tccacaagag cggcggaccg ccagggcttg caagatcgaa acagagcggc agcggaatgt
    12481 aatcgtcggc gctgaggtcg ataagccgac gatattcttc gacggcagct tgaggttcac
    12541 tggcgaccag cagtcgagcc agaccacggc gcaggaaaat ttccgatgca cgctcgtcgt
    12601 atcccgctgc caccgtccgt tcgccactgc gttcgatcgc atcgcgcaac agcgcttccg
    12661 cttgatcgcg atttcccgtc agaacgcccc agcgcgacag ctgtagcacc ggcggtgcat
    12721 acgtcggatc gaggtcaatc gccttcagat acgcttgctg cgccccggtc atgtcgccga
    12781 gcgattcgag aatccgtccc tgatagtcgg aatagcgaca cagcaccgat ggggtcactt
    12841 tgggcgtgcc gtcgggctga acggatcgca acagctcatc gatttcggtc cgggcttcca
    12901 gatagcgacc gattcgaaac agttcttgcg acagaacgaa gcgggcgcgg tgcaaaagct
    12961 cctcgggcac gctggcttcc ggcctggtcg ccgcttcaat cgactgacgt agcagcttga
    13021 tcgccgcttc gggctggagc agcacttccg caaacagccc cgccaaaatc agctcgcagc
    13081 gcgccacggt cgcaagctgc gccgcattgc cggcttgtcg cagccgctcg ataaacgact
    13141 ggagcagcgt caccgccgca tcccagcgcg ccaagcggac atacgctgcg gcgaccagct
    13201 caacgacttg tgattcatcg ggctgaagct tcagtgcttc gccgagcagc gcttcggctc
    13261 cgtcgctgtc tccggaccgt tcggccaggg ccgcctgcgc acagagcaaa agcgcgagcg
    13321 gttcgggcac attccgtcga cgatactgct tttccagttc gcggcaggtc ggggcaaagt
    13381 cgtatcccgt ctctggcaaa agacacagcg agacgagcaa gtggagcgcc gccaagtttt
    13441 ctggccaaat ttcaagcgcc agtcccactg attcacacgc gacgcgcagt tcaccgactt
    13501 tcgacgctgc gtgggctcgt ttggtgtgga aagtcgatcg ttccgacggc tctccctcgc
    13561 gagcggcgcg caccaaaagg acttgtcccg tctgatacgt ccgataaaaa tcgccgcgct
    13621 ggtaatacac atgaaacagc gcgcggtggg ctggtagaca gtctttgtcg acggtgagcg
    13681 ctcgttcgta gtattcaatc gcgcgctcgg gttggtgcag tttgtcgagg taaatttgcc
    13741 caatgtgcgc caaaatgccg gcctgttctt tgggctcggt ccacagcttg gactccagtt
    13801 ccaaagtctg cgtcacccgc gcatagtctt cccgtcggac gtagatatcg cgcaggctgt
    13861 gtagcgccgg caaacaggcc gccgacgtcc gcaccgccgc ttcgtaatag cgaatcgcct
    13921 cgtcttcttt gccaaacttc gcttcggtga ccgacccgca cttaaagtag agcagcgcct
    13981 tgtgttgcct gtcagtgacc agccgaatct gccgtcgcgt cacctcgacc agctcggccc
    14041 agcgctcatt ggcttcgagc agtcgcgcca tcgcgtcgag cgcttcgcgg tgagtgggct
    14101 gtgcttcgac gacgtgtcga taggccgaga tcgcccgatc gtggtcgcgc aggccttctt
    14161 cgcacaaaag accgattcgc atgtgcagcg cgatgatctg ctgctcctcg cttgccagcg
    14221 tcacccgcgt cgacagcagt gccaccagct tttcgtagtc acgcgcgctt tcgtagtggt
    14281 gttcaagcgc atcgagcgat tcggtgtcgg tgggatcgac cgcatgcagt ttttcgtaga
    14341 agcgcaccgc ctcgatcacg tcgcggagct tggcggtggc gacacgggca gcgcggcgaa
    14401 gaatttcgac tcgcttgctg tcatcgcgga caagctgcgc ccgtcgctca tacgccgcca
    14461 acagttcttt ccactggttg cgcccggcat acacccgctc gagcgtcgct tgcgtggtgt
    14521 cgttttcggc gtcggactct agtgctttga ggtaactgcc aatcgcctca tcgacttgct
    14581 gcagatactg aagctgaact tgtccccgac ggagatagag gtcagcaggt cggtacgatc
    14641 gcgtcttctg ggtttcaatc aggcgcagcg ccgcatcgta aattttcagc gtttcggtcc
    14701 actgctgccg tcgatagcaa gcccgctcgt aggccgtaaa cgcggcccgg ttcgccggat
    14761 cgaggaaaaa tgcctcgccg ttgagtcggt acgcatcgtc cgcctcgccc agcttgtcga
    14821 gaagctcacc gagtgcaaac agatagcgcg accgttcact gggttcctga acctgccgca
    14881 gcagcgtggt cagcgttcgt cgcagatcgg cgtattgttc ggtctgggtg tacaatgtga
    14941 ccaaagaagc cagcgctgtt cgctcctcgg gccactcact gagcactttt tcgaacgtcc
    15001 ggaccgcttc ggcattttgc gacagaaact cgcgctgcac ttggccaaga cgcagcaaaa
    15061 gctgccgttt cagatcggct tcatcgacgc tgagcttgct cttcggtttg tcgatgcggg
    15121 aaagcgcgtg cccaagcaac tgggcttgct gggcatgacg cccacttgcc ccgtagcgca
    15181 gctccagttg cagcaacatt ttgctgctgc ccaggatcat ctcgggcgaa agctcataaa
    15241 gccgttccca gatgcgaatc tcgtcggcgg gacgctccag ccgcgcatac agctccgcaa
    15301 gctgcgtaag cacctgtcgc tgttcgtcgg gaccggcaag ctgcggcaaa agctcttcga
    15361 gagtacgcgc cagtccgtcg ggctgaccga gcttttcata ggcttcccca agtacgcgca
    15421 gcaaatacgg cgtcttgggt gcccgctcga gcgtttcggc aatcaaacca taacgcccgt
    15481 cgacaagaca gagactgacc aggctggcga tcgcgccttc gtgggccggt gaaagctgca
    15541 gcgcttcgct atacgcatca atcgcctgtt cgataagcgg tgccgacgcc tcgccgccga
    15601 tcagcttgcc ttggtgttcg gccagttgcc ccagctcgat gagcagctcg aatcgctggt
    15661 gatcgtcgcg caggagcggc aaaagtcggc gcatggccgt cagctgttcg gttttttgtc
    15721 ccagcgtgcg atagagcgaa accagggcgc gggccgccgg acgacagcgt ggatcgcggc
    15781 ggagcgcgcc ctcataggcc gtcagcgctt ccgacagtgc cgcggtgtcg ccgccttggc
    15841 tggccttctg ttcaaacagc gcgcccagct cacacgcgag cagtgccgcg gcctgcgcac
    15901 cgacgggcgc actggtgtca ccaagcccca gcttgcgtgg cgacagcatc gccatcgtcg
    15961 acggaagtgc agctgatggt tccccgccga tggtcagcgc cgcttcgacc gccgtgcgca
    16021 acagctcaat cgcttgcttg gtgtcgccac gctgctgaag cagtcgcgcc agtgcatgca
    16081 tcgcgaccac gtccgtcggg tcacgctcaa gcacttcttg ataacagact tcggcccgct
    16141 gcaaatcacc tgcgtcggtg gcgtgatgcg ccagctgctt gagcacgcgc agcttgcttc
    16201 gtggactttg gacgcgattg agaagctcga cgagtgcttc gacgagttcc ccgtcgtggt
    16261 ttcgtcgctg acaggtcagc accctggcga tcgagataag cagaccacct gcggcgtcgg
    16321 caaatggctc cgcttgcgcg agcagactcg acagcagtct gggcagctcg gtttcgccag
    16381 caaccgcttc gggaagtggg tcgggcagca gcaccgccgc caagatggcc gcaaccgatg
    16441 cataggtcgc agccagcgtc tgtccgtcag tatcccctgc ggaaaccgaa tcggcaagcc
    16501 gacgatagac gccgagcagt cgagccacat cgctgcgtcg tcgaaactgc cgcgccaagg
    16561 tctgtagggc agggcgatcc gcgagctggg gcagcgacag cagaccttcg cgctcggtcg
    16621 cttgcagcgc ttcggcggcg cgggcggcgt gtgccaccaa gatcgcgcgt tcggggctgg
    16681 ccggcggaca ggcttgtgcg cacgccaaca gccactcaca gacggaccga cggtcattgc
    16741 cttgcagaag ctgcgtagcg atgcgcagca tcaacacttc gtcgacaatg ccaaagctga
    16801 gcagtcgcgt ggcctgctgc accgcctggc tgcgcgcctc gtcaccttgc tggggaatgg
    16861 ccagaatcca gcactcgacg gcgcgataca ggtgtgccat cgcgagcgcg cggttccact
    16921 cgtcgctgct gctctctgct gccggtgccg ggagtccttc gatcagtcga ttcgcttggt
    16981 gctgccagta ctgcgcagct tcaccaaatc gaccgacacg ttccagttgc tgcgccgccc
    17041 gtcgctcgat caggtgttgg ctaagctgcg cggtcagcgt cgggtgaatg ctcgtcacct
    17101 gactgatgct gacgagcaga tcggcggcct gggtcgccat cacttgcgag ttcatcgacg
    17161 ctgtcgcaag ttctagcgcc gtcaccagca gctcgaccgg cggactggct ccccgcagca
    17221 ccggatcacc gccgagcagc gcgaatcgtt cgttgagcaa ctgccactgc cccggaccat
    17281 acgccggcag gctggccaag tgatgttcac tggcgcgcag cgcaagcacc atgcccgcga
    17341 gatcgctgcg tttcggcgtt cgctcggcct gctgggcaag ctgctgcacc gaaagcatga
    17401 ccaccgacgg atcactgctg ccttgaccgg cctggacgcg cagcgaggct gcccacagca
    17461 gatcatttcc gtcgacgctc gacgaaagcg tatcggcagc ccggaccgtc agcgcctgcg
    17521 atgtcaaaag cagggcgtaa atccgcggcg ctgaaccttc ggcgagcagc gtttcggcca
    17581 gatcaagctg gccttgccac agcagaatct ctcccagttc gcttttttcg tcggcagagc
    17641 cgtggcggat cacccagtcg gtatagtccg caagctgtgg accctgggcg cgtccttcaa
    17701 gagcagcttg caggcgcagc atcgaagcaa ccgggtgatg tgccgtgcca agcagtgcga
    17761 gcgccgcatc cgctttgcct tgcccgtccc acaaaagcat cgccatccga accgcaagct
    17821 cggcgtgttc cgtcggatct tgcgtttcgc caagttcggc cagcaaaagc tgaaggagat
    17881 cgtcgagcac aggctgggct gcctgaccga cgagcgcctg caagcgggcc tcgggggacg
    17941 gggatctcgc tgattccgcg ggcagtgtgc cagacggttc agccgaaggc gcagcgcttt
    18001 ctgacatgtg ttgtgagagc ttaacacatc gtcaatgcac aaaatactgg cttgccggct
    18061 tgggtttggc gtcggcagcg gcctgcaatt tggggggatt aggctggacc gggtccgtct
    18121 gagttttcgc gccgcgccgc gctgatcagc gacgggacac ctgacacgcc ggctcgacca
    18181 ccacgggcga tcaggcgacc catgtcttcc atttcgagcg acgctgagcg acgcaaaagc
    18241 gtcatgttga ttggcgtatt ggcatcggca gcgaggaagg ccgcacgcag caccgcgttt
    18301 ttgatgtaac cgccggaaat ctcgtagtct ttggcgagcg ctttccagtt gatgtctttc
    18361 gcccacggcg cattttgcgg cagcatcgac ttccacagcc gctctctctc gggcggttcg
    18421 ggaatttcca gatgcaggtg cagcgtcagt cgtcgctgga gcgcttcgtc gagcgatgct
    18481 tctttgttgg tcgtcatcac gacgacgccg gtgtactgct ctagtcgctg gagcagatag
    18541 ttgactgcga ggttgccgta gcgatcgttg gatgtcttga cttcggtacg cttggcaaac
    18601 agcgagtcgg cttcgtcgaa caaaagcacc gcgtgcg
//
LOCUS       DAORGN010000086        18628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1200_length_18628_cov_5.112206, whole genome shotgun sequence.
ACCESSION   DAORGN010000086 DAORGN010000000
VERSION     DAORGN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18628
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_18628_cov_5.112206"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKL17_11115"
     CDS             <1..493
                     /locus_tag="PKL17_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45327.1"
                     /translation="GLINYQFLPRNLATALTLLPKLLPSYPYVQVSYHGLALWFTSPA
                     LLFLLWPQRPQWSSAPPHPPVVDGGERLRGLRFVLLLTVVPIALASLLYQNSGFVQFG
                     YRFSLDYLPLLIILLRVGSPRLADSWLFRAAVVFGVVINLLGALSFNRMPQFYFNGFF
                     PVS"
     gene            568..1725
                     /locus_tag="PKL17_11120"
     CDS             568..1725
                     /locus_tag="PKL17_11120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNK45328.1"
                     /translation="MREVVILQGARTPFGRGVKGSLKDTRPDDLGIHVLRGALSRSGV
                     APADIEDVVLGCAFPEAEQGMNVARLVAVGAGLPVEVPAMTINRFCSSGVQAAAVVAD
                     RIAVGQIDFGIAGGLESMSMIPMTGAKVSLNPRLVEEQSGLFTPMGVTAENVARRFQI
                     ARAEQDEFAYQSHQKAVRAQQAGFFDGEILPIETSVFDDSGKARSIKVDRDELPRADT
                     DAAKLSGLRPAFDPTGSVTPGNSSPLTDGAAALVMAAKDKVGGRPFLGYFRGFAVAGV
                     PPEIMGIGPVPAVRKLLAKTGLTVADIDVWELNEAFASQAIYCARELGVPSEKLNVNG
                     GAIAIGHPLGATGARMTLTALRELGRRGGRYGIISMCIGGGMGAAALVERA"
     gene            complement(1787..2875)
                     /locus_tag="PKL17_11125"
     CDS             complement(1787..2875)
                     /locus_tag="PKL17_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45329.1"
                     /translation="MASSRPTPLQTLVAQDCNRMAWRGQSGHYEVWFLTLAHRESKTG
                     FWIRYTLEAPEAGHGPPYAELWFCRSDGKEPDKNFGIHRRFDIADLHSQHRPFVVRIS
                     GSELGHSHASGSLSGNGHTIRWDLHWDGQAKPHLLLPKALYANPMGIAETIVLSPNHS
                     ISVSGSIEVDGQRYELSGTPGGQSHLWGKKHAYGWAWSRCNAFDREDERLHDKPKAVF
                     ESLTARLRRGPVVLPVTLLSVYPDGLDGPEVAFKEWADLPLCRAEYRTGSYVMSAEGP
                     RTGIEVQLSCSADDMVRTDYVDPDGTPAFNHYAGAASCTLLLKKRMCPGAAWRIERTF
                     RSDQGAQFEWAGRAGDSLVRKRHVLVND"
     gene            3289..4569
                     /locus_tag="PKL17_11130"
     CDS             3289..4569
                     /locus_tag="PKL17_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45330.1"
                     /translation="MANVLVMLEFLDSEPLCASLEALGQARRLGSALGLTVYALVPMQ
                     SIAPQKRQQLGQLLGYYGADSVVMLTSDAPRAESELRFAPYARALLQACDELPPKLWL
                     MADTPAARDVAPRLAARLGGVFLSHGEPVCEHAGLRIYDADGQPLAVSDDPLADEMFA
                     PMMVPVVMTLPAGRYALSRGSTAVPVRFMAALDGDPASERTPVPGGGFSEESHDPHSR
                     SARLWFQSGDKPSEDAMPPLFVVAEDALPFDRRLAYTAIGIGPRSAEREDVQHALPIA
                     ESAPSGLRVAMEAALAKPRERTVEPALANLLPFDDQSDSEPAISVGRSWDASDISTGD
                     TEPRTPAAPASDSALPVVPPVVVEPVAPFPLATASDSEIWDGAPTVDAEKADRDAAPD
                     AELDGIDTEPIEDTGTAPSKDPSAGKSEQREETP"
     gene            4566..5228
                     /locus_tag="PKL17_11135"
     CDS             4566..5228
                     /locus_tag="PKL17_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45331.1"
                     /translation="MKVLLLLHSAPTARAVACAKSIGDVTALAVAPSDAVFWAGGDLS
                     GCQKVRVWDESLDPATLHAFDWEARVASVVAQAARRLGTSVVVLTESQAGFLGAALAE
                     QLNLAHLSEVISARVEAASDDEPLLHVRRRGLHGVQVLSGVAQAVLSVLPSWGSASSV
                     APSEPGSPSEVWSLEDISLCSGDLPPPQLEAGAKMARRHRPRLYIGAEALVERLRRDG
                     LE"
     gene            5289..6983
                     /locus_tag="PKL17_11140"
     CDS             5289..6983
                     /locus_tag="PKL17_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45332.1"
                     /translation="MGADRAASTERLGDVSRLLRARSFWIDGETPAAQRVRSNLQSGP
                     ARIDREAEAWISTGRLPSDRVGIALAEGSGLLGPNSFGFLNNVDGIAPLSAPLAERPR
                     LGRVALVVPRRDALPELAPLLSSRLLGVSWLVSVGDGDPAEVLQFLQHDPATGGLLVA
                     LGKGARPSSLLDSLVGKPAVVLLSPSQRDSALLRAVSRRALAKVTSSFEEWLAHGALL
                     DAGFPSQLSRPAMRRDKTSGRPASVIVLGAGADLVQRELSALHWPPPLRVDADDADAV
                     ARTLAKAAEQADLLILCGSRDQLADVRPARAAILLEPSERDRLRALLLAIQSVTTPTL
                     PHTPVVVRPLRDRLETVLADLPPPLYVAGEVVSREPLGDHDVKRLLAAYGVRVSRQAP
                     VNSTTSALRVVGKLGTPVWVLPGLLPMDDVSKLAAAEARDGVLCQTQAEVKRQTAQLL
                     ERCECVLLREVPPKGPSLRVQAVSERGVGLTLRVSPVGDETGSEACLLPLLSDEAQAV
                     ARSFVPDEDASQLESLAQLLGQIAVCFESQNIEGDLLIRLADEPLVVQAAGVLLRG"
     gene            complement(6980..7918)
                     /locus_tag="PKL17_11145"
     CDS             complement(6980..7918)
                     /locus_tag="PKL17_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNK45333.1"
                     /translation="MTFSEPSTLLGPLCALLSSLAWAIGSSVYTRQAGRVGALEVNLT
                     RAAIATPIFFVATLLTVGSDGISALSPKHLGWLSLSVICSYGLGDSMFYTAALRLGTP
                     TALAIGSIYPAWATLFGALTLGEPVGGQRLLGTLLCVAGVIWLVLLQAAERGAQATQT
                     NKNNRLVGVLLALLTSLFWAGNTYSVRRGGLGVAPFVVNGYRYFLATIGLGVIWLGRY
                     RRLGPKASEGERLLLSPKEMGRFAPAVLLEAFIGSSIFVFAMTHTDLSIAAPLAALSP
                     LFSVPIGVLLGTETLNLRRVLAILVTVAGVVCLVTA"
     gene            complement(7915..10623)
                     /gene="hrpB"
                     /locus_tag="PKL17_11150"
     CDS             complement(7915..10623)
                     /gene="hrpB"
                     /locus_tag="PKL17_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNK45334.1"
                     /translation="MSASPSPPLPTSPISHALLPHPREPLPIDPLLPEIVARLSETPN
                     LVIAAPPGAGKTTRVPQALLSLISDGEIVVLEPRRIAAQLSALRVAEELGESLGQTVG
                     VQLRFESITSAATRLRFVTEGVLLRQLASDPLLPTVKLVVFDEFHERHYQTDLLLALL
                     VRLQRTSRPDLRLCAMSATLHTTKVAEFLGQCPLIESQGKSFPVDIEYLPLPTDEPLP
                     KQVRRAVREVVQKEKLSPQNDTARRQGDILVFLPGSAEIRRCQEALSELEPQFGLDIL
                     PLHGDLSLAEQRQVVAPRPPSAPRRVILSTNLAETSLTIDGVTVVIDSGLHRQAGYDH
                     WSGLPTLKVVPISRASATQRAGRAGRTQPGRCLRLYTKHDHDLRPAFDSPEILRMDLS
                     EPLLLLHALGLRPGDVSFLDPLPQKMVDAAESLLSCIGATTAKTDGSRLTDIGWKLTQ
                     LPLHPRLARLLLAGAESGIIDDAAVVAALLSERDILQNGRAFLKDPHRDKAASSERSD
                     LTHRLDRFRFAESVRFSRPSLSREGLDADTLVRVDRARRQLVDRAKQFAKPHRLSVAE
                     REQTLERAVLLGFPDRVARRRKKPSGESSELVLASGGSAQQSEQSLVRDAEWLVLVDV
                     EERGGQTAPVARLLSAISPDWLLDVPGDFLVEQDDLTWNGTAGQVERSTKLRFGQLVL
                     DESRVPAAATDGKVLAMLSERATADAENIFSDGEKVAGLLSRLSVLVATYPELGLPMP
                     NETHQQAAIVGLCVGKTSLAELRSDSLQAAVLSALCKDTPSSPTQIGRLLEEHLPDSI
                     KLPGGRRTRIHYVANQPPFIESRLQDFFGMSDGPKLAQGRLPLVIHLLAPNQRAVQVT
                     TDLRGFWARHYPTIRRELMRKYPRHAWPEDPLTAEPPQPKGPRPPR"
     gene            complement(10724..12358)
                     /gene="ggt"
                     /locus_tag="PKL17_11155"
     CDS             complement(10724..12358)
                     /gene="ggt"
                     /locus_tag="PKL17_11155"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNK45335.1"
                     /translation="MAADHELASQAGAEILAQGGNAVDAAIATALALGVVQPAGSGLG
                     GGGFLLVRKADGTVAALDFREVAPKQASRDMFLDAATGKAVPERSRWGGLAVAVPAEP
                     AGLAHALREWGTFKPGQVIAPALRLADKGFPVGKHLARSASMVVPKLADDHPLRSFLA
                     PQGKILTVGQTVRRPELAGTLRILGQEGFLAFYKTTPGSIGDDLITANRQAGGLLQAD
                     DLTSYKPIVRTPIAGRYRNHQVFVMPPPAGGITAIEALQILDARPPLAQGPAASATLH
                     DTIEAFKHAFADRAKLHGDPAFTNIPVAELASTEYAKGRAATILADRVLPPSSYGRPG
                     TDKPLSPPRDKGTSHLCVIDRNGNAAALTTTVNLSFGARVVGAKSGVLLNNQMDDFTA
                     KPATENAFGLVGDEANLVAPGKRPASSMTPLIAVGPDGSVYCAGGSGGPTIVTGVVQT
                     LINLIDFGMEAEAAVSSPRIHAQFLPDNVLVEPEVPADVRERLSQRGHKLVVTPAPLE
                     TAVQVVVYHPARDGKPASFSAASDPRKGGKPATSAR"
     gene            12675..14747
                     /locus_tag="PKL17_11160"
     CDS             12675..14747
                     /locus_tag="PKL17_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNK45336.1"
                     /translation="MSASENPKGKAPLTMKIKFKSANLEQFIERYSVDVSKGGIFIRT
                     KEPQSVGTQLKFEFQLQDGAPLIVGEGTVVWNRAPDPSKPSLAPGMGVRFDKLTPDSQ
                     ERLDQILSEKTRRGEGGVESRFDAGVRESRGDAAPSQITPTASPASGRNEFGDEPTRA
                     MAADQVNKLAEAMQLGGMIDEDAPTKANPAAVAEALRKKAADTPALVPMTPASAKAGL
                     SKTLLGQGFGMMTPPLKPAVGPAAAVLPPAKPIAPPAEPARGIPESAKTPTASVEEQQ
                     QRIADSSRTPTGSAATPPRAVETPAAPATPAVAATPATPPSASTPVGVSPAGAAAADK
                     VESDDGPISKPAPMGPPPKLDAPESLFSDAAPPPMTAQPVKQGGSGGLIAVVGALALL
                     LGGGGAYYALVLSKKPVDNGPPTMIGVTTAPNTGSTGNTGNAGSTGTNPTGPTTQPTE
                     VKPPEPPVKPPEPTSVPVKPDKPIEGTEVTTDPPGAQIEFDGKQFGPTPTRIPGLAAG
                     KQLRITMPGFQETKIKLKANPKDGEKLSYPLSAVERMVEVTSTPKGADVYLDGKKVGK
                     TPFQIKKLDTKEKHEIELRKGGFASWTRTVSSADTFEVKKGKEVLTVEATLAASTDAG
                     KKGKTAKKEAPAAAEPAGSAPVKPEAPAEAKPKAEEKPKAEEKPKAEEKPKAEEKAEK
                     PAEDKPAS"
     gene            complement(14828..15967)
                     /locus_tag="PKL17_11165"
     CDS             complement(14828..15967)
                     /locus_tag="PKL17_11165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK45337.1"
                     /translation="MVNLLYVAAFCHAALALLLWFNRARTPRLPEVKAHPDRPKSVVV
                     IVPARNEESNIGPCVRSILLQDYPALTVRVVDDHSTDHTGAIVGKLAEYDRRLTLIHP
                     PSLPPGWLGKPHAVHNGSLGATADYLLFLDADVRLEPSAVSRAVALAEAKQAGLTTVM
                     PELVAESFWERATQPLVGLLLYGLLDPVRVNDPNKDSAAGFGPFMLFRRDAYEKLGGH
                     QAVRSEIVEDLKLSQLVKAQRLGLCVAHGTDVVKLRMYDSLSSLLAGWTKNFHVALGP
                     LKALAPLFAILIAAVFTLPTVMFWTLIGLWAATGHLPHFATAAALAYGADWCARLSLH
                     SAYDITPRGVRAVGGLVVGYILCSSVYRALFGRPVTWRGRSYQAQ"
     gene            16057..16482
                     /locus_tag="PKL17_11170"
     CDS             16057..16482
                     /locus_tag="PKL17_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45338.1"
                     /translation="MGTLSSTLLLLLAALASAEGIGKLWPASRFWLSALSPLSGLLGV
                     GSVATGLVTIVEMAAYLGFVRVAPMVYLGFLGGGVLSFLLGLRFGYALLLSWFGDKLP
                     AGLRHTLDDVQAWLIHNERSLGYLGLATGMFCLLLNVFT"
     gene            16583..18598
                     /locus_tag="PKL17_11175"
     CDS             16583..18598
                     /locus_tag="PKL17_11175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNK45339.1"
                     /translation="MRHVSIFALALPLCAPLVNPSFAVAQKPPAAVQAAPSAPQKPAQ
                     DKPSVLVPSDNLVVSGIPPVPLRVAERVGRYTEFRTAGFSDWHPVRREQLILTRFADV
                     PQVHQVAMPGGARTQLTFFPDRVSHASYEPTKGDYFLFEKDVGGGEWFQIFRFDLGTG
                     EITMLTDGKSRNMGMVWNQTGSEIAYASTRRTGKDMDLWVMNPLDKASDHLVLELTGG
                     GWAPLDFSPDGKKLILQEELSINESYLWMVDLAKKEKTLLTPKGGADKVAYGRARFAK
                     DGKGLYLTTDKESEFQRLAYVDLATQKYTFLTTTIPWDVEDFDLSHDGKNLAISTNEN
                     GESRLYIMPTQTKQLRPLREIPVGVMGGVSFHRSGSELGFTLSSARASADSYSVNVAT
                     GKLTRWTQSESGGLRTQEFSEPKLVKWPSFDGREISGFLYLPPKKFAGKRPVIVNIHG
                     GPEAQFRPGFIGRNNYLLNELGVALLFPNVRGSSGYGKTFLKLDDGDKREGSYKDAES
                     LYKWLKTQPELDGDRIMVTGGSYGGHMTLVSATQHSPLIRCALAVVGMSSLVTFLEKT
                     EGYRRDLRRVEYGDERDPKMREFLQRIAPLNLAKNVQKPLFIVQGQNDPRVPASEAEQ
                     MIQTIKQNNVPAWYLLAKDEGHGFAKKKNQDFQFYSTVLFIEEYLLK"
BASE COUNT         3481 a         5556 c         6040 g         3551 t
ORIGIN      
        1 cggcctgatc aactaccagt ttttgccgcg caacctggcg acggcgctga cgctcctgcc
       61 gaagctgctg ccgtcgtatc cgtatgtgca ggtgagctat cacggcctgg cgctgtggtt
      121 tacgtcgccg gcgctcttgt ttcttttgtg gccgcagcgt ccgcagtggt cgagcgcacc
      181 cccgcaccct ccagttgtcg acggaggcga gcggctgcgc gggctgcgtt tcgtgctttt
      241 gctgacggtg gtgccgattg cgctggccag tctgctgtat caaaacagcg gctttgtcca
      301 gttcggctat cgcttcagcc tcgattacct gccgcttttg atcatcctgc tccgtgtggg
      361 cagtccgcgg ctggccgatt cgtggctgtt ccgtgccgcg gtggtctttg gcgtggtgat
      421 aaacctgctg ggggcgctgt cgtttaaccg aatgccgcag ttctatttca acgggttttt
      481 cccggtcagc taaccagcgc cagaacgggc cttcgaccga agacggtagt gcttgcgagc
      541 cgcgaggctt tggctcacac tgccgccatg cgtgaagtcg tcattttgca gggtgctcgt
      601 actccgttcg gtcgtggtgt gaaaggctcg ctcaaagaca cgcggcctga tgatctggga
      661 atccatgtgc tgcggggcgc gctgtcgcgt tcgggcgtgg ctcccgccga cattgaagac
      721 gtggtcctcg ggtgtgcatt tcccgaagca gagcagggca tgaacgtggc tcgtctggtt
      781 gcagtcggtg cgggtcttcc cgtcgaagtt ccggcgatga ccatcaaccg cttctgctcg
      841 tcgggcgtcc aagccgcagc cgtggtggcc gatcggatcg cggtgggaca gatcgacttt
      901 ggcatcgcgg gtggtctgga atcgatgtcg atgattccga tgaccggcgc caaggtgtcg
      961 ctcaacccgc ggctcgtcga agagcagtcg ggtctgttta cgccgatggg tgtgaccgcc
     1021 gaaaacgtgg cgcgccgctt ccagattgcg cgtgccgaac aagacgagtt cgcctaccaa
     1081 agccatcaga aagcggtgcg tgcgcagcag gccggctttt tcgatgggga aatcctgccg
     1141 atcgaaacct ccgtgttcga cgacagtggc aaagcgcgca gcatcaaagt cgaccgcgat
     1201 gagcttcctc gggccgacac cgatgcggcc aagctctctg gcctgcgtcc ggcgttcgat
     1261 ccgacgggat cggtgacgcc cggcaacagc tcgccactga cggacggtgc cgccgcgctg
     1321 gtgatggccg ccaaagacaa ggtgggcggt cggcctttcc tgggttactt ccgcggtttt
     1381 gctgtcgctg gcgttccgcc ggaaatcatg ggcattggtc cggttcccgc cgtccgcaag
     1441 ctgctggcca aaacgggact caccgtcgct gacattgatg tgtgggaact gaacgaagcg
     1501 tttgccagcc aggccatcta ctgcgcccgc gagcttggcg tgccgagcga gaagctgaac
     1561 gtcaacggcg gcgcgattgc gattggccac ccgcttggtg cgaccggcgc ccgcatgacg
     1621 ctgaccgcgc tgcgtgaact gggccgtcgc ggtggtcgct acggcatcat ttcgatgtgc
     1681 attggcggcg gtatgggcgc tgctgctctc gtcgagcgag cgtaactcca tcgggtcgcc
     1741 cgccttcccc ggcgaccgca cggcgcgtgc ggactgcgtg caccgcctaa tcgtttacga
     1801 ggacgtggcg cttgcggacg agtgaatcgc cggcgcgacc ggcccactca aactgggcac
     1861 cttggtcgct gcggaacgtc cgttcaatgc gccacgccgc gcctggacac atccgttttt
     1921 tgagcagcag ggtgcagctt gcggcgcccg catagtggtt aaacgcgggc gtaccatcgg
     1981 gatcgacgta gtcggtgcgc accatgtcat cggcgctgca cgacaactgg acttcgatgc
     2041 cggtgcgtgg tccctcggcg ctcatcacgt agctgccggt gcggtattcg gcgcggcaga
     2101 gcggcagatc cgcccactct ttgaacgcga cttcggggcc gtccagcccg tcgggataga
     2161 ccgacagaag cgtcaccggc agcaccaccg gaccacgccg cagccgcgct gtcagcgatt
     2221 caaacaccgc tttgggtttg tcgtgaagcc gctcgtcttc gcgatcgaag gcattgcagc
     2281 gcgaccaggc ccagccgtaa gcgtgttttt tgccccacaa gtggctctgt ccaccgggcg
     2341 tgccggaaag ctcgtagcgc tgtccatcga cttcaatcga accagacacc gagatgctgt
     2401 ggtttggcga cagcacgatg gtttccgcga tgcccatcgg gtttgcgtac agcgccttgg
     2461 gcaaaagcag gtgcggtttg gcctgcccgt cccagtgcag atcccagcga atcgtgtgac
     2521 cgtttccgga cagggaaccg ctcgcgtggc tgtggccgag ctcggaaccg ctgatgcgga
     2581 cgacaaacgg tcgatgctgg ctgtggagat cggcgatgtc gaagcgtcgg tgaatgccga
     2641 agtttttgtc cggttctttg ccgtcggatc ggcagaacca cagctcggcg taaggcggac
     2701 cgtggccagc ctcgggcgct tccagggtgt agcgaatcca aaagccggtc ttgctttcgc
     2761 ggtgggcgag cgtcaaaaac cagacttcgt agtgaccgga ctggccgcgc caagccatgc
     2821 gattgcagtc ttgggcgacg agcgtttgca gcggagtggg ccgtgaggat gccatgatga
     2881 atctacgttg agatgcgaag cgctcaggct gtttgcgctg gggcgccgat cagttgggcg
     2941 aaaatacacc attttacggc aagggctgcg gcggcggtgc gcttttcgac ggagcccaaa
     3001 acccggattg ctcccgcttt tttgccgtgt cgaatcggaa tgtttgtcgc ttttctgcgc
     3061 gactgtgtcc gttttggccg caaacgactg cgcgggcggt cgatttgcgc agaaaaactt
     3121 gcgatttcgt cctactctgt gtgtagttgt cctacatggt ggctgcagat ttttcatgga
     3181 agaaaggacg agccaccgcc gaagagagga cttttgtata cttgcgtcgg tgtagccgca
     3241 tcgtgttagg ccaatccctg gtccttggcg gcagataggt gttagcccgt ggcgaatgtc
     3301 ttggtcatgc tggaattcct cgacagtgag ccgctttgtg cgtcgctcga agcactgggt
     3361 caggctcgcc ggctcggtag cgcgcttggg ctcacggtct atgcgcttgt gccgatgcag
     3421 tcgattgcgc cgcagaagcg ccagcagctc ggccagttgc ttggctacta tggcgccgac
     3481 agcgtcgtga tgctgacttc cgatgcgccg cgggccgaaa gcgagctgcg gtttgcgccg
     3541 tatgcgcggg cgcttctgca agcgtgtgat gagctgccgc cgaagctgtg gctgatggcc
     3601 gatacgcccg cggctcggga tgtggcaccg cggctggcgg cacggctcgg tggcgtgttc
     3661 ctttcgcacg gtgagccggt ctgcgagcac gcgggcctgc gaatttacga tgcggatggc
     3721 cagccattgg cggtgtccga cgatccgctt gccgacgaga tgttcgcgcc gatgatggtg
     3781 ccggtggtga tgacgctgcc ggcgggtcgc tatgcactgt cgcgcggcag taccgcggtg
     3841 ccggtgcgct ttatggcggc gctcgatgga gacccggctt ccgagcgaac ccccgtgccc
     3901 ggcggtggtt tttccgaaga aagtcacgat ccacattcgc gcagcgctcg actgtggttt
     3961 cagagcggag acaagcccag cgaggatgcg atgccgccgc tgtttgtggt ggccgaagac
     4021 gcgctgccgt ttgaccgacg gcttgcatat accgcgatcg gcatcggtcc gcgatcggca
     4081 gaacgagaag acgtgcagca tgcactgccc atcgcagaat cggcgccttc aggacttcgt
     4141 gtcgcgatgg aagccgccct ggccaaaccg agagagcgca ccgtcgaacc ggcgctcgcg
     4201 aacctgctgc cctttgatga tcagtcggac tccgagcctg ccatttcagt cggacgttcg
     4261 tgggatgcaa gtgatatttc gacgggcgat accgaacccc ggacgccggc tgcgcctgcg
     4321 agcgattccg ctttgccggt cgttcccccg gttgtcgtcg agcccgtggc gccgtttccc
     4381 ctcgcgacgg ccagcgacag cgaaatttgg gacggggcgc cgacggtcga tgccgaaaaa
     4441 gccgatcgtg acgcggcgcc cgacgctgaa ctggatggca tcgacaccga accgatcgaa
     4501 gacaccggga ctgcgccaag caaagatccg tccgcgggca agagcgagca gcgggaggag
     4561 acgccatgaa ggtccttttg cttcttcatt cggcgccgac tgcacgcgcg gttgcctgcg
     4621 ccaagtcgat tggggatgtg accgcgcttg cggtggctcc gtcggacgct gtgttttggg
     4681 ctggcgggga tttgtctggc tgccaaaaag ttcgcgtgtg ggatgaaagt ctcgatccgg
     4741 cgacgctgca cgcttttgat tgggaagcgc gggttgcttc ggtcgtcgca caagcggcac
     4801 ggcggctggg tacgtcggtg gtggttttaa cggaaagcca ggccggtttt ctcggcgcag
     4861 cgctggctga acagctgaac ttggcgcacc tgagcgaagt gatttcggcg cgcgtcgaag
     4921 ccgcgtcgga tgacgagccg ctgctgcatg tccgacggcg tggtctgcat ggcgtgcagg
     4981 ttctgtcggg agtggcgcaa gcggttctgt cggttttgcc gtcttggggc agcgcaagct
     5041 cggtggcacc cagtgagcca ggatcgccgt cagaagtctg gtcgctcgaa gacatttcgc
     5101 tgtgctcggg cgacttgcca ccgccgcagc tagaggctgg cgcgaagatg gctcgtcggc
     5161 atcgtccgcg gctttacatc ggcgccgaag cgcttgtcga aagactgcgc cgcgatggtt
     5221 tggagtaagt acaaatggcg cgcgtccgca tcaggtcgaa cactgcagct tcaccggccc
     5281 cccaattggt gggcgccgat cgtgctgcgt cgacggaacg gctcggtgat gtgagccggc
     5341 ttttgcgggc gcgcagcttt tggatcgacg gggaaacgcc agccgcacag cgggtgcgca
     5401 gcaacttgca gtcagggccg gcgcggatcg atcgcgaagc cgaggcgtgg atttccacgg
     5461 gccggttgcc gtcggatcgc gtcggaattg cgcttgcgga aggcagcggg ctgcttgggc
     5521 cgaatagctt tggttttctc aacaacgtcg acggaattgc gccgctttct gccccacttg
     5581 ccgagcgtcc gcgcctgggc cgggtggcgc tggtggttcc tcgtcgcgac gcgctgccgg
     5641 aacttgcgcc acttttgtcg tcacggctgc tcggcgtgtc atggcttgtt tcggtcggcg
     5701 acggtgatcc agcggaagtg ttgcagtttc ttcagcacga tcccgcgacg ggaggtctgc
     5761 tggttgcgct cggcaaaggt gcgcggccca gcagcttgct cgacagcttg gtgggaaagc
     5821 cggcggtggt gttgctttcg ccgagtcagc gtgacagcgc gcttttgcgg gcggtgtcgc
     5881 gacgagcgct cgcgaaagtg acctcgagtt ttgaggagtg gctggcgcac ggtgcgcttc
     5941 tggacgctgg ttttccttcg cagctttcgc ggcccgcgat gagacgtgac aaaacaagtg
     6001 gccggcccgc ttctgtgatt gtgctggggg ccggtgcgga cttggtgcag cgtgagctgt
     6061 ccgctttgca ctggccaccg ccactgcggg tcgatgcaga cgatgcagat gccgtcgcgc
     6121 ggacgttggc caaagcggcg gaacaggcgg atctgttgat tctgtgtggc tcgcgcgatc
     6181 agcttgcgga cgtgcgtccc gcgcgcgcgg ccatcctgct ggagccgtcg gagcgagaca
     6241 ggctgcgggc gctgcttctg gcgattcaat cagtgacgac gccgacgctg ccgcatacgc
     6301 ccgtcgtcgt gcgtcctttg cgcgatcgac tggaaaccgt cttggcggat ttgccgccgc
     6361 cgctgtacgt cgctggcgaa gtggtaagtc gcgagccgct cggggatcac gacgtaaagc
     6421 ggctgttggc ggcctatggc gtgcgggtgt cacggcaagc gccagtcaac tcgacgacgt
     6481 cggctctgcg ggtggttggc aagctgggga cgccggtttg ggtgctgccg ggactcttgc
     6541 cgatggacga cgtgagcaag ctggctgcgg cggaagcgcg cgacggcgtg ttgtgccaga
     6601 cgcaggccga ggtcaaacgg cagacggcgc agcttctgga acgatgcgag tgtgtgcttc
     6661 tgcgtgaggt gccgcccaaa ggcccgtcgc tgcgtgtgca agcagtaagc gagcgcgggg
     6721 tcggtctgac gctgcgagtg tctccggtcg gcgacgaaac gggtagcgaa gcgtgtcttt
     6781 tgccgctgct ttcagacgaa gcccaagcgg tggcgcgttc gtttgtgccc gacgaagatg
     6841 cgtcgcagct tgaatccttg gcgcagcttt tgggacagat tgccgtctgc ttcgagtcgc
     6901 aaaacatcga aggcgatctg ctgattcggc tcgcggatga accgctcgtg gtgcaagccg
     6961 cgggcgtgct tttgcgcggc taagcagtca ccaagcagac gacaccagcg acggtgacaa
     7021 ggattgccag cacgcgccgc aggttgagcg tttccgtgcc caacagcaca ccgatcggca
     7081 cggaaaacaa gggcgacagg gcggccagtg gtgccgcgat gctcaggtcg gtgtgggtca
     7141 tcgcaaagac aaagatcgag ctgccgataa acgcctcgag gagaaccgcc ggggcaaagc
     7201 ggcccatttc tttcggtgag agcagcagcc gttcgccctc gctcgctttc ggtcccagtc
     7261 gacggtagcg tccaagccaa atcaccccga ggccaatcgt cgccaggaaa tagcggtagc
     7321 cgtttacgac aaaaggtgcg acgccgagcc cgccacgacg gaccgaatag gtgttgccgg
     7381 cccaaaacag cgacgtcaaa agcgcgagca gcacaccaac gagacggttg tttttgttcg
     7441 tctgcgttgc ttgcgccccg cgctccgccg cttgcagaag caccagccag atgacacccg
     7501 ccacgcagag cagcgtgccg agcagtcgct gtccaccgac gggttcaccg agcgtcagcg
     7561 caccaaacag cgtcgcccag gccggataga tcgatccaat cgccagtgcc gtcggtgtgc
     7621 cgagccgaag cgcggcagtg taaaacatcg aatcgcccag cccgtaagag cagatcaccg
     7681 acagcgaaag ccagcccagg tgtttgggcg aaagcgccga aatgccgtcg ctgccgacag
     7741 tcagaagcgt tgcaacaaag aagatcgggg tggcaatggc ggcccgcgtc aggtttacct
     7801 caagcgcacc gacgcgaccg gcttgccggg tatacaccga cgatccgatc gcccacgcga
     7861 gcgaactgag cagggcgcac aaaggcccga gcagggtgga tggttcggaa aaagtcatcg
     7921 cggcggtcgt ggtcctttgg gctgcggtgg ctcagcggtc agcggatctt ccggccacgc
     7981 atgccgtgga tattttcgca tcagttcgcg gcgaatggtt gggtaatggc gtgcccaaaa
     8041 accgcgcaga tccgtcgtga cctgcaccgc gcgctggttt ggcgcgagca gatgaatcac
     8101 cagcggcagg cgtccttgcg ccagcttggg accgtcggac atgccaaaaa aatcctgcaa
     8161 acgtgactcg ataaacggcg gttgattggc aacgtagtgg atgcgcgtgc gccgaccgcc
     8221 tgggagcttg atcgaatcgg gcaaatgctc ttccaacaaa cggccaatct gggtcgggct
     8281 cgacggagtg tctttgcaca gcgccgaaag taccgccgct tggagcgaat cgctccgcag
     8341 ctctgccaag ctggtctttc cgacgcacaa tccgacgatc gcggcttgct ggtgcgtttc
     8401 gtttggcatc ggcagaccga gctctggata cgtggcgacg agcacgctca agcgtgacag
     8461 aagaccggcg actttttcac cgtcggaaaa gatgttttct gcgtcagcag ttgcgcgttc
     8521 actgagcatt gccagcactt tgccgtctgt ggcggcggct ggaacacggc tttcatcgag
     8581 cacgagctga ccgaagcgaa gcttggtgct gcgttcgacc tgaccggcag tcccattcca
     8641 tgtcagatcg tcctgctcga cgagaaagtc accgggcaca tcgagcagcc agtctggcga
     8701 aatcgcgctc agcaggcgcg ccaccggggc ggtttgaccg ccgcgctcct cgacatcgac
     8761 caaaaccagc cactcggcgt cgcggaccag gctctgttcc gactgctgtg ccgatcctcc
     8821 cgacgccaag accagctcgc tggattcccc gcttggtttt ttccgccgtc gtgccacgcg
     8881 atccggaaat ccgagcagca ctgcgcgttc gagcgtttgc tctcgctcag cgaccgacaa
     8941 gcggtgcggc ttggcaaact gcttcgcccg atcgacaagc tggcgacggg cgcgatcgac
     9001 ccgcacgagg gtgtcggcat ccagaccttc acgcgagaga gacggacgcg aaaaccgcac
     9061 cgactcggca aaacgaaagc gatccagccg atgggtcaga tcggaacgct cgctcgaagc
     9121 ggctttgtcc cgatgcgggt ctttcaaaaa tgcgcggcca ttttgcaaaa tgtcgcgttc
     9181 gctcagcagc gccgccacca ccgccgcatc gtcgatgatg ccgctctccg cgcccgccag
     9241 cagcagacgc gccaagcggg gatgcagcgg caactgggtc agtttccatc cgatgtccgt
     9301 cagccgcgat ccatccgttt tggctgtcgt tgccccgatg catgaaagca gcgactccgc
     9361 ggcatcgacc attttttgtg gcagcggatc caggaagctc acgtcgcctg ggcgcagacc
     9421 cagcgcgtgc agtagaagca gcggttccga cagatccatg cggaggattt cggggctgtc
     9481 gaacgcgggc cgcagatcgt gatcgtgctt ggtgtaaagc cgtaggcagc gtcctggctg
     9541 ggttcgtccg gcccgacccg ctctttgcgt cgcagaggct cggctgatcg gaaccacttt
     9601 cagcgtcggc agccccgacc agtgatcata tccggcttgg cggtgcagac cgctgtcgat
     9661 gaccaccgtc acgccatcaa tcgtcagcga cgtttcggca aggttcgtcg acaaaatcac
     9721 ccgacggggc gcactcgggg gccgcggcgc caccacttgt cgctgttcgg ccaggctgag
     9781 atcgccatgc agtggcaaga tgtcgagccc aaactggggt tccagctcgc tgagcgcttc
     9841 ctgacagcga cggatttctg cgctgccggg cagaaacacc aagatgtcac cttgccggcg
     9901 cgcggtgtcg ttttgcggcg acagcttttc tttttgcacc acctcgcgga ccgcccgtcg
     9961 aacttgcttg ggcaacggtt cgtcggtggg cagcggcaga tactcaatgt cgacgggaaa
    10021 tgacttgcct tggctttcga tgagcggaca ctggcccaga aactcggcga ctttggtggt
    10081 gtgtaacgtc gcggacattg cgcacagtcg caagtccggg cggctcgtcc gctgaagccg
    10141 cacaagcagc gccagcagca ggtccgtctg gtagtgacgt tcgtgaaatt catcgaacac
    10201 caccagtttg accgtcggaa gtagcggatc cgacgccagc tgccggagca acaccccttc
    10261 ggtgacaaag cgcagccgcg tcgccgcact tgtgatcgat tcaaagcgaa gctgcacacc
    10321 gacggtctga cccagcgatt cgcccagttc ctcggcgacg cgcagcgccg aaagctgcgc
    10381 ggcaattcgg cgtggctcca gcaccacgat ttccccatcg gaaatcagcg acaaaagcgc
    10441 ctggggcacc cgcgtcgtct tgccagcacc cggcggcgca gcaatcacca ggttgggcgt
    10501 ctcgctaagc ctcgcgacga tttcgggcag cagcggatcg atcggcagcg gctcccgcgg
    10561 atgcggcagc aaggcatgcg aaatcggaga ggtgggcagc ggcggggacg gcgaagcaga
    10621 catcggctcc tgcgaaacaa gaaacggcgc accatactgc cgtcagcggc ttgggtaaag
    10681 ccgggcaacg cagtgcgtca caaaaacgcc caagtcgccg aggttagcga gcagaagtcg
    10741 cgggcttgcc ccctttgcgc ggatcgctcg ccgccgaaaa actggcgggt tttccgtcgc
    10801 gggcagggtg atacacgacc acttgcacgg cggtttcgag cggcgctggg gtgacgacca
    10861 gcttgtgtcc gcgctggctc agcctctcgc gcacgtcggc gggcacttct ggttcgacca
    10921 gcacgttgtc gggcagaaac tgtgcgtgga tgcgcggcga cgagactgcc gcttcagctt
    10981 ccatgccaaa gtcgatcagg ttgatcagcg tctgcacgac tccggtgacg atggtcggtc
    11041 cgcccgagcc tcccgcgcag tacaccgaac cgtcgggccc aaccgcaatg agcggtgtca
    11101 tgctcgacgc ggggcgcttg cccggtgcca cgaggtttgc ttcatcgccg accagaccga
    11161 atgcattttc cgtcgctggt ttggcggtaa aatcatccat ttggttgttg agcagcacgc
    11221 cgctctttgc gccgacgacc ctggcgccaa atgacaggtt caccgtcgtg gtcagcgctg
    11281 cggcattgcc attccgatcg atcacgcaca ggtggctggt gcctttgtcg cgcggggggg
    11341 acaggggctt gtcggtgccg ggccgaccgt agctcgacgg tggcagcacg cgatcggcca
    11401 agatcgtggc agcgcggcct tttgcatact cggtgctcgc gagttctgcg acggggatgt
    11461 tggtaaatgc cggatcgcca tggagctttg cgcgatcggc aaaggcgtgc ttaaacgcct
    11521 cgatggtgtc gtgcagcgtg gccgatgctg ctggtccctg cgcgagcggc ggcctggcat
    11581 cgagtatctg cagcgcttcg atcgccgtga ttcccccagc ggggggcggc attacgaaga
    11641 cctgatggtt gcggtagcgg ccagcgatcg gcgtgcggac gatcggcttg tagcttgtca
    11701 aatcgtcggc ttgcaaaagt ccgccggcct gccggttcgc ggtgatgaga tcgtcgccaa
    11761 tcgagcccgg agttgtcttg taaaaagcca aaaaaccttc ttgtccgagg atgcgcagcg
    11821 ttcctgccag ctcggggcga cgcacggtct gtccgacggt gagaatcttg ccttgcggtg
    11881 ccaaaaagct gcgcagcgga tgatcgtcgg ccagcttcgg taccaccatc gaggcactgc
    11941 gtgccaggtg ctttccgacg ggaaatcctt tgtcagccag ccgcagcgct ggcgctatca
    12001 cttgtccggg cttgaatgtc ccccactcgc gcagtgcgtg cgccagaccg gcgggctcgg
    12061 cgggaacagc gacggccaag ccaccccagc gggatcgctc gggcaccgct ttccctgtcg
    12121 ctgcatccaa aaacatgtct cggctggctt gtttgggcgc gacctcacgg aagtccagtg
    12181 ctgcgaccgt cccatcggct ttgcgcacaa gcagaaaacc gccaccaccc agaccggagc
    12241 ccgccggttg gaccactccg agtgcgagcg cggtggcaat tgccgcatcg accgcattgc
    12301 cgccctgcgc aaggatttcg gcgccggcct gggatgccag ctcgtgatcg gctgccactg
    12361 cgccgctact tccggtgacg gtgtgtggat aagcggcttg tggcgacgcc tcacttccga
    12421 aaagcgcgct gagcagagcg gcacagacaa gccgtgggcg ccggaatttt cgacgtgaat
    12481 acatatggtt caacgaaaaa atgtgagtca tgttattgtt ttttatcgcc tctattggct
    12541 tgtctgcgat ccagggggtg gggttggcgt cgcgccagct catttctccc atacaagtta
    12601 cgacagggaa gctggtttac acaaagacgg aagagcagtg agcggtagag cgcaatcctc
    12661 gattgggagc aaagatgtcg gcctctgaga acccgaaggg caaagccccg ctgacgatga
    12721 agatcaagtt caagtccgcc aacctcgagc agtttatcga gcggtattcg gtggatgtca
    12781 gcaagggcgg gatcttcatc cgcaccaagg aaccgcagtc ggtggggacg cagctgaagt
    12841 tcgagtttca gctccaggat ggcgccccgc ttatcgtcgg tgagggcacg gtggtttgga
    12901 atcgtgctcc cgacccgagc aagccgtctt tggcgcccgg catgggggtg cgtttcgaca
    12961 agctgacacc cgatagccaa gagcgactgg atcagatttt gtctgaaaag actcgtcgcg
    13021 gcgaaggcgg ggtggaatcg cggttcgacg cgggtgtgcg agagtcgcgt ggtgatgcgg
    13081 ctccgagcca gatcacaccg acggcgagtc cggccagtgg ccgcaacgag tttggcgacg
    13141 agccaacccg cgcgatggct gccgatcagg tcaacaagct cgccgaggca atgcagctcg
    13201 ggggaatgat tgacgaggac gcaccgacga aggccaaccc ggctgcggtg gcggaagcgc
    13261 tgcgcaaaaa agcggcagac acgccggcac ttgtgccgat gaccccagcc tcggcaaaag
    13321 ctggtctgtc caagacgctg cttgggcagg gatttggcat gatgacgccg ccgctcaagc
    13381 ctgcggtggg accagccgcg gcggtgctac cacccgccaa gccgatcgcg ccacctgccg
    13441 agccggcgcg cggaattccc gagtcagcca agaccccgac cgcatcggtc gaagaacagc
    13501 agcagcggat cgctgattcc agccgtacac cgactggatc ggcagcgacg ccgcctcggg
    13561 ctgtggaaac cccggcggct ccggccactc ccgccgtcgc agctacgcct gcgactccgc
    13621 cgtctgcttc gacgccagtg ggtgtaagcc ctgcgggcgc ggcggcagcc gataaagtcg
    13681 aatccgatga cgggccgatt tccaagcccg cgccgatggg tccgccaccg aagcttgatg
    13741 cgcctgaatc gctgttttcc gatgctgcgc cgcccccaat gacggcgcag ccggtcaagc
    13801 aaggtggcag cggcggtctg atcgcggtcg tcggtgcgct cgccctgctg ctcggtggtg
    13861 gtggtgcata ttacgcgctg gtgctaagca agaagccggt cgataacggt ccgccgacca
    13921 tgattggtgt gacgacggcg cccaacaccg gatcgactgg caacactgga aatgctggct
    13981 cgacgggcac gaacccgacg gggccgacga cgcaaccaac cgaagtcaag ccgcccgagc
    14041 cgccggtcaa gccgcccgag ccgaccagcg ttccggtcaa gccggacaag ccgatcgagg
    14101 gaaccgaggt cacaaccgat ccgcccggtg cgcagatcga gttcgacggc aagcagtttg
    14161 gtccgacgcc gacgcggatt ccgggccttg ccgccggcaa acagcttcgc atcacgatgc
    14221 ccggttttca ggaaacgaag atcaagctga aggcgaatcc caaagacggc gaaaagctga
    14281 gctatccact ttcggcggtc gagcgaatgg tcgaagtcac ctctacgccc aagggcgctg
    14341 acgtgtatct cgacggaaaa aaggtcggca agacgccgtt tcagatcaag aagctcgaca
    14401 ccaaggaaaa gcacgaaatc gagctgcgca aaggcggttt tgcaagctgg acgcgcacgg
    14461 tgtcgtcggc ggacacgttc gaagtgaaga agggcaaaga agttctgacg gtggaagcga
    14521 cgcttgcggc gagcaccgac gctggcaaaa agggcaagac agccaaaaaa gaagcgccag
    14581 ccgcggcaga acccgcaggc agtgctccgg tcaaacccga agcgccagcc gaagccaagc
    14641 cgaaggccga agagaagccg aaggccgaag agaagccaaa agccgaagaa aagccgaagg
    14701 ccgaagaaaa agccgaaaaa ccggcagaag acaagcccgc aagctaactt gggctgattt
    14761 tcgccacgtt ccgccgaatt tcggcaaatt cctgctgatt cccccgattt tgctgtcgct
    14821 tggctggtta ctgcgcttgg taggatcgtc ctcgccaggt gacaggacga ccgaacagcg
    14881 cgcggtacac cgacgaacac aaaatgtagc cgacgaccag tccgccgacg gcgcgaacgc
    14941 cgcgtggggt gatgtcatac gccgaatgaa gtgacaggcg ggcgcaccag tcggcgccat
    15001 atgccaaggc tgctgcggtg gcaaagtggg gcaggtgtcc cgtcgctgcc cacagaccga
    15061 tcagggtcca aaacatcacc gtcggcaggg taaacaccgc agcgatcaag atcgcaaaca
    15121 gcggcgccag tgcttttaac gggccaagcg cgacgtgaaa gtttttggtc cagccagcca
    15181 gtagcgacga cagcgagtcg tacatccgca gcttcaccac gtcggtgcca tgcgctacgc
    15241 acagcccgag tcgctgcgct ttgacaagct gcgaaagctt caggtcttcg acgatttcgc
    15301 tgcgcaccgc ctgatgtcca ccgagctttt cgtaggcatc gcgacggaac agcatgaacg
    15361 ggccaaaacc tgcggcgctg tctttgttcg gatcgttgac ccgcaccgga tcgagcagac
    15421 cgtagagcaa gagcccgacg agaggctgcg ttgctcgttc ccagaaactt tcggcgacca
    15481 gctcgggcat caccgtcgtg agtcccgctt gcttggcttc ggccaatgcc accgcccgcg
    15541 ataccgccga tggttccagc cgaacgtccg catcgagaaa caacagataa tcggcggtcg
    15601 ctccgagaga gccgttgtgc accgcatgcg gcttgccgag ccagcccggc ggcagcgacg
    15661 gcggatgaat cagcgtcaag cggcgatcgt attccgccag cttgccgacg atcgctccgg
    15721 tatggtctgt cgagtgatca tcgaccacac gcaccgtcag cgccggatag tcttgcaaaa
    15781 ggatgctgcg gacgcacggc ccgatgttcg attcttcgtt gcgagccggc acgatcacca
    15841 ccaccgattt gggccggtca gggtgggctt ttacctcggg cagccgcggt gtgcgcgccc
    15901 ggttaaacca cagaagcagc gcgagcgcgg cgtgacagaa cgcagcgaca taaagcaggt
    15961 tgaccatcgc ggggaaccta gcacacacct ggccgtgtgc cgcgcgggct gtgataatga
    16021 agcgcaagcg ggtttatcac caaaccggag acgcgcatgg gaactctgag cagtacgctg
    16081 cttttgctct tggcggcgct cgccagcgcc gaaggaatcg gcaagctctg gccggcatcg
    16141 cgcttttggc tgtcggccct gtcgccgctg tcgggacttt tgggggtcgg atcggtggcc
    16201 actgggcttg tcaccatcgt tgaaatggcc gcctacctgg ggtttgttcg cgtcgcgccg
    16261 atggtgtatc tgggctttct gggcggcggg gtgttgtcgt ttttgcttgg tctgcgcttt
    16321 ggttatgcgc tgctgctttc gtggtttggc gacaagctgc ctgcggggct gcgccacacg
    16381 ctcgatgacg tgcaggcgtg gcttatccac aacgagcggt cgcttggcta cctggggctt
    16441 gcaacgggaa tgttctgttt gctgctcaac gtgtttacct aagctgcgcc cgttgggttt
    16501 ctgccttttg ccgttctctg cggctcagtc ggatcgaccc aagtcgtgat acagagtgcc
    16561 gcacatggag gtatttcccc tgatgcgaca cgtttcgatt tttgcgctgg cactgccttt
    16621 gtgcgcgccc cttgtgaacc cttctttcgc ggtggcgcaa aagccacctg ccgcagtgca
    16681 agcggcccct tctgcgccac agaaacccgc gcaggacaaa ccatccgtcc ttgtcccttc
    16741 ggacaacttg gtggtgtccg gaattccgcc ggtgccgctg cgtgtggcag agcgcgttgg
    16801 tcgttacacc gaatttcgca ccgcaggctt ttccgattgg catccagtcc gtcgagaaca
    16861 gctcatcctg acgcgctttg ccgatgttcc gcaggtgcat caagtcgcca tgccgggcgg
    16921 cgcgcgcacc cagcttacgt tttttcccga tcgcgtgtcc cacgccagct acgagcccac
    16981 caaaggcgac tacttcctgt ttgaaaaaga tgtcggcggc ggggaatggt tccagatttt
    17041 ccgtttcgat ctgggaaccg gtgagatcac gatgctcacc gacggaaaaa gccgcaacat
    17101 gggaatggtc tggaatcaga ccggaagcga aattgcctac gcttccaccc gtcgtaccgg
    17161 caaagacatg gatctgtggg tgatgaatcc gctcgacaaa gcgagcgacc acttggtgtt
    17221 ggaactgacc ggtggtggct gggctccgct cgacttttct cccgacggaa aaaaactgat
    17281 tctgcaggaa gagctttcga tcaacgaaag ctatctgtgg atggtggatc tggccaaaaa
    17341 ggaaaagaca ctccttacgc caaaaggagg cgcagacaag gttgcgtatg gacgggcgcg
    17401 ctttgccaaa gacggcaaag gactgtacct gacgaccgat aaggaatcgg aatttcagcg
    17461 cctggcctac gtcgacttgg cgacgcaaaa gtatacgttt ttgaccacga ccattccgtg
    17521 ggatgtggaa gacttcgatc tgtctcatga tggaaaaaac ctggcgattt cgaccaatga
    17581 aaacggagaa agccgcctgt acatcatgcc gacgcagacc aagcagcttc gcccgctgcg
    17641 tgagattccc gtcggagtga tgggtggcgt ttcctttcat cgcagcggca gcgagctggg
    17701 atttaccctc agctcagcgc gtgcgagtgc ggattcctat tcggtgaatg tcgcgaccgg
    17761 aaaactgacg cgctggaccc agagcgaaag cggcggactg cgcacccagg aattttccga
    17821 gcccaagctg gtcaagtggc cgagcttcga cgggcgagag atttccggat tcctgtatct
    17881 gccacccaaa aagtttgctg ggaaacgtcc ggtgatcgtc aacatccacg gtggtccgga
    17941 agcgcagttc cgtcccggct ttattggtcg caacaactac ctgctcaatg aactgggcgt
    18001 cgcgcttctg tttccgaatg tgcggggttc ctcaggctac ggaaagacat tcctaaagct
    18061 tgatgatggc gacaagcgcg aaggttccta caaagatgca gagtctttgt acaagtggct
    18121 gaaaacccag ccagagctag atggtgatcg catcatggtg acgggcggaa gctacggcgg
    18181 acacatgacg ctggtgtcgg ccacgcagca ctcgccgctt atccgttgtg cgctcgcagt
    18241 ggtgggaatg agcagtctgg tcacgttcct ggaaaaaacc gaaggctacc ggcgtgatct
    18301 ccgccgcgtc gagtatggcg acgagcgcga ccccaagatg cgcgaattcc tacagcgcat
    18361 tgcgccgctc aatctggcga aaaacgtgca gaagccgctg ttcattgtgc agggacaaaa
    18421 cgatccgcgc gtcccggcaa gtgaagcgga acagatgatc cagacgatca aacagaacaa
    18481 cgttccggcg tggtatctgc tcgcgaaaga tgaagggcac ggcttcgcga aaaagaagaa
    18541 ccaagacttc cagttttact cgacggtgct gttcatcgaa gagtatctgc tgaagtagga
    18601 ctttccgccg ccgcgtgttt cgtctacc
//
LOCUS       DAORGN010000087        18613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1203_length_18613_cov_5.696735, whole genome shotgun sequence.
ACCESSION   DAORGN010000087 DAORGN010000000
VERSION     DAORGN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18613
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1203_length_18613_cov_5.696735"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PKL17_11180"
     CDS             complement(<1..1049)
                     /locus_tag="PKL17_11180"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK45340.1"
                     /translation="MATLDPVVKSGTQPHVVIIGGGFGGLTAAKQLDGANVRVTLVDR
                     CNHHLFQPLLYQVAMAGLSPADIAAPIRSVVRFQPNVSVLLAEVQDIDVNARRVVLDR
                     GDLRYDYLIVAAGAQNHYFGHDDWAKYAVGLKDIDDAVEVRRRVLLAFEAAERTVDPA
                     ERERLLTFVVVGGGPTGVELAGALGELSRYVLARDFRNINPAQAKIILIEGGPRILSS
                     FPDALAMRAVIDLGHLGVEVRAGLRVTKIDGRGVEASGKFIPTATVLWAAGVRGSPLS
                     AKLGGEIDRAGRVIVNPDLSVAARREVFAVGDMAAFLHQDGKPLPGVSPVAIQQARHV
                     AKNLLADLDQEPRTP"
     gene            complement(1025..2863)
                     /locus_tag="PKL17_11185"
     CDS             complement(1025..2863)
                     /locus_tag="PKL17_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45341.1"
                     /translation="MPSTARPALFFRRAETLTLSALSLLLAFGGPASVGSALAQSAPV
                     LPAAAPPAASAPVAPVAQPAAATPGAAKAKPAAKPLPKAPAAAKPPAGPKPLALPPKS
                     PVLSKSDVYQRAAPATVFLIAKDGSRFNTALGVIVKPNGVVVSDSRLVSGVEKGQVFA
                     FLYDAAFAGDEDPLIFLRANQAKALPVQVVRMDAPNHLLLLQLPEPPIKQQYRSLDLF
                     DVKTATAVGADVIGLRTHGRQTLAMMSGSIMAVRPGIIETDPPLTLDHTGGPLLSPTG
                     RLLGIVTFADKAVDTPGVARPVELLYSLLTGSVGSAVTAPTNLTPEPAAESRNAVEAA
                     RIALGNALAQKLDKRPALLLHSEFINSMAIRGRVPLRDFDSIEFVSGLISAITKDSPA
                     KAKVVSELFPLLVTDKKGRAYRRDGAKYTMVPTSDFGVVAIDDITGTLFTTDNKRSLI
                     YYDAEGKSWRPTHITPVSQVAASGGTVYAILEDGRLITATTDGKDSIQLWPKLIKGGN
                     VVASQGSMYLFEGEKIYRYRNGTWDNKMKPVAFAMQQLVVRGDSYYGLDLNGRVFSST
                     AQRYIDRDANIYKLWPIGRDLLVITKDNQRFYYSSEEDQWRPWTQW"
     gene            3057..3749
                     /locus_tag="PKL17_11190"
     CDS             3057..3749
                     /locus_tag="PKL17_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HNK45342.1"
                     /translation="MTNLLRIESQARRELSVWQRFFAPELVQTLGPVEAQNVQGELDS
                     ELKKLLAMMPDPGWRAPQMRAFGLGGAIYLALYLVLARRGQTADQVWHICEQATRTRF
                     SSLSWFQRKTMGWLLFSPLWKQMTKSIATKSAQAAVGGWVLDYVPPDPGSSDYGVTYH
                     RCAIRDLAHAVGAEAFAPFICQSDAVMSELLGWGLRRTGTIAQGQSQCDFRFRKGQPT
                     DVRSVTRLPVVR"
     gene            complement(3809..5398)
                     /locus_tag="PKL17_11195"
     CDS             complement(3809..5398)
                     /locus_tag="PKL17_11195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNK45343.1"
                     /translation="MFDPSTSPFAGLGLIPELCRAVAESGYTSPTPIQTQSIPILLKG
                     HDLLGCAQTGTGKTAAFALPILQLLYAQRQPPPPVEHKPARHPPQARKIRALVLSPTR
                     ELASQIEESFRVYGAHTGITSTVIFGGVKQGAQVQRLRMGIDILVATPGRLLDLMGQG
                     FVSLKDLQFFVLDEADRMLDMGFIHDVRKVLAALPDVKQSLLFSATMPPDIEALAMSF
                     LRSPQRVAVAPVSSTAERISQSVYKVAKNDKPKLLAHLLKDANLSRVLVFTRTKHGAN
                     KVVKQLAESGYVGEAIHGNKSQNAREAALAHFKSGETRILVATDIAARGIDVDNVSHV
                     INYDLPNEPESYVHRIGRTARAGAEGIAIAFCDWDEQGFLNSIEKLTRQKVPVVPDHP
                     FAKSNRGGEPAAVPSDRPPRREPREQSGPRPPSSHGTRPQPQRPQGERSGGSPGGPRP
                     PRPDNGSRPHGGSKPQPSHARKPDDRGAGPRPHGRPQSGDKGGSRGQNRSGGGGDRSR
                     GQSSSDRERIERQLDWMKRYT"
     gene            complement(5529..8366)
                     /locus_tag="PKL17_11200"
     CDS             complement(5529..8366)
                     /locus_tag="PKL17_11200"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNK45344.1"
                     /translation="MERTPSSTLPATNAEALAALREQLEAAVQEFATDKHKVGELLGL
                     MVSDVDRAMREPLEIIPVCHHSPSSALHVAKRLAERPPKVIFMEMCEDLRGTIEGLRD
                     CRLPVALQAFAQTADAFPKGWSPLSVVAPLTEFSAEFQAIAYALENPSTELVFVDRAV
                     DYVFQWMPQKEDELEKHMVKEEERDQATPKAPGEEEDSDVPDHGAAIGLQMGNVEPTF
                     DVFLSFLLKNARVRHFTEWWDQYVEQAVLAADYPTYRFVLSLVGSLLRRLGRKDEDHE
                     NDKRRERYMWTRMKEHLKKTGIAPSDAIYLCGAVHAVSEVPEFGTHSPAVWDIPKATP
                     TKWLYGLIPSSYIAIEHQFHHPAGTVALSETTWEKGIAALSVKPWKLSKKASSPATSS
                     GKASKAKAAPAVSAPAPIGKADTARVLGFFVKPPLLHSADEEQLLRWSVDIVSLARDN
                     GYLTSTADAIAIYQTSMLLANLRNRQHPSPYDFEDAAITCLEKDRQVKKRNVPKLCQM
                     LLGGNRAGRVGYSALPPLAQDVYARLKPLGVNLEASQIQRALLDFKKDPHLLPASDLL
                     WMLHYLTQGHPVLRPIMGERKLGHVPVQESWDVSIGKYQESIIRLGYEGVTVEYVVEK
                     RLKTEAFGPQAKTVTALKAAEDSILFLKSERLTEELGDRAVDLLRNEMSAQDAPKVVE
                     RIRRLVHYYRATPAGLPKWLKDFVSTGYSHYSMLLPKGFEDRGTRPHEVASMLSFVFD
                     FEPQALALGCQRSQLDIAIRQAGPVTTDPAKLGLLWASELLLKLRDIETIREFLAQTL
                     DNPLMLPVFPEYVSGFLLALQFAPVIGRVVVELLSKAFARLPEDILYPWLPGLLLSLR
                     PYAEDLLPKLLKDVNGLYPKDTKTLSAWAFPWDKKAAAPAPAGDSASAPGEAATTSAS
                     ASVTLSAEEQAVVSLLQTNPITAARLAAILP"
     gene            complement(8403..9740)
                     /locus_tag="PKL17_11205"
     CDS             complement(8403..9740)
                     /locus_tag="PKL17_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK45345.1"
                     /translation="MATPRTKKTKTDDSAAAAAPAGTPATDSAAPAPAAAETSTSASA
                     EFPIATKAEATASASVTVEVKAEVKETPKAKKAEVLRQPAEVKYAEELAYLASIDTGK
                     RPFSWKLSPRMIRTFILGSTPADKIERPIAQKFFGDPALVERAIVTLASDRGLLLIGD
                     PGTGKSWLAELLAAAISGSSTHTVQGTAGTTEDQIKYSWNVALVIAKGQSKESLIPSP
                     IMIAMQHAGMGRIEELTRCTSDVQDALISILSEKYITIPELSGDNVVFARPGFNIIAT
                     ANSRDRGVNDLSTALKRRFNFVTIPTVTNKKSEKEIVLFRTRELLTRNQFEVELPPAL
                     LDILLQTFSDLRDAQTAATSDDQRLESSLSTAEQIGVLEDAVLHSQFFGQTTLSAGNL
                     AKALVGTLVRRIPEEVSVMNKFWHSVVDKRAKEKKGEWEEYLEGGKLAMSLLK"
     gene            complement(9752..11227)
                     /locus_tag="PKL17_11210"
     CDS             complement(9752..11227)
                     /locus_tag="PKL17_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK45346.1"
                     /translation="MTDTTKPASPTPSVDLQNRQQALLWRLVAACFGQGEKAQNLEAL
                     SKEIAKEVDVPDLVLDPMVSITTLTQRFPELRKDFETPLPGAMLDDESPGGVALTSAN
                     PDGSGNAAADSASDKDRLRRTLVLSKLLLNAFGPQTQTKTVTAQQYSQWTQDLGRLEE
                     AFGYPQGGLRGRGGGGAGGAGGTGPGGGRRLLSEDDLRRGFQNLESELVRRMELREVL
                     KDSRLASKLSPSIALVEQLLADKANLSGVALENAKKLIRAYVDQLADVLKLQVQKASK
                     GKLDRSIPPKRVFRNLDLKKTLWKNLTNWNPDEQRLYVDRLTYRRTANKTTPTRLIVV
                     VDQSGSMVDAMVQSTILASIFAGLPKVEVHLVAFDTRVLDLTPWVHDPFEVLLRTELG
                     GGTYIRLALIEAAKKIVEPKNTAMVLISDFYEGGSDQELLDFIKGLKDSGVHFIPVGA
                     LQSSGYFSVSEFFRTRLAELGMPILHGAVNKLIHQLRYLLN"
     gene            complement(11254..12666)
                     /locus_tag="PKL17_11215"
     CDS             complement(11254..12666)
                     /locus_tag="PKL17_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45347.1"
                     /translation="MSHTLTALATLPPGVAETAEVIARLDHCFFTGFGRMSEREVAAV
                     SALPRLLAATPLGPRVKEAVDALLRSEFVEKHFATLACARAALQGAMADSLSAQASTA
                     LGRTPNLGEPSLSTPESPGHHGVFRESARHFLMEIALTGFGQLEPESLYPFYATLDKI
                     QEEPALIRLSAILSGFLSELLSALPIPTGKTVPTYRWVDLWSRAMLLSLSVQPAAKIE
                     TVSGELLPLGCDLHHHRNLFSVCLHGLLRVGSDVRYVRTTVSAYKVDTVIAHEMWRLL
                     EGSYDKLLKGIRDCAMIKLTDMQMLGTGDLLWDESRAALGPSYSPFDIAESFLAPSAK
                     LTRPSLSGLDRHPAQLAELFFLRDFKTVKIESHKSKRGSAAELVEEQALEVSGTKLPL
                     SGARLSDSSGMTMERIAKASALVGLLRFDRGRWSVQPLAIKIGSQVELAGVPPQSLKS
                     KDDTVAVLRERAGKLLRKKS"
     gene            complement(12703..13194)
                     /locus_tag="PKL17_11220"
     CDS             complement(12703..13194)
                     /locus_tag="PKL17_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45348.1"
                     /translation="MALLPPEITSLPLSEPMSRPRKVKDVPYVELFGGRVQGVVSSGS
                     DENRVYVSFFEAGSSVNFNCSTNNNRPCSGCRGTPCKHLAQLMSEAVLQFGAETVARY
                     LKLSGDLSKFTSARDIMLQVRGVQSRLDVSQVFSRFLSHLRYFELASSNQPIPEMTWF
                     VSG"
     gene            complement(13362..14408)
                     /locus_tag="PKL17_11225"
     CDS             complement(13362..14408)
                     /locus_tag="PKL17_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK45349.1"
                     /translation="MRFLPTNVVRILSVLPLLAVLGCTTYTTESTEVGVRTRKLFGSG
                     IEPHIYEPGATYMFFPWMSDWATFDIMTQKLEMTAAKERGDREGDDAIVLKSRDGNDI
                     SIDVTVTWRIDPQRAPAILARGARSTALLKEELVRPACRAYVRDSFNELHSEEFFERK
                     QLLEKAQKAKTKLTEELTSEGVIIEDVMPGEQRFNPEYERVIHERKIAEQNSERLKSE
                     AQAAEAEQARQLEKARGDVQAQIAEAKGKVERIRIGADRALYENERTAKALLAEATAR
                     AKAIEKQNKAMSGAGGRTAVKLRIADALAGKPILIVPAATGTNIQKLDVTRIVEAVIA
                     KESTSGSSSAHESE"
     gene            complement(14408..15442)
                     /locus_tag="PKL17_11230"
     CDS             complement(14408..15442)
                     /locus_tag="PKL17_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK45350.1"
                     /translation="METLVNVVKYLLSQRWIRIPLILFLGGWWLSSCCTVYIGPNEVG
                     IHAISFGSKSGIQKEPWQTGTHVVIPNYERVLIFPTDLQALNRSANPTESSKSSRSDG
                     PIVIQTADNYKVSVDATVLYRIHDPYRVITTIGPGRMFESVVVIPRSDNALRKALGEL
                     TAEEFYLGDKRLKQTQAATEMLRTEFADKGIELVKILIRQFSYDKAYQHQIEQRKIQD
                     QTVLKNRAESAAAREDAHKREVISTGQAAVQVEREKGSREIAQLIADANLYQRTKAAE
                     GDLLVKSAQAQGTEMENHALEGAGAQAMVGQKLADLMKGVRVIIVPSTGQGSTNPLNI
                     SELLRQFEVK"
     gene            complement(15471..16067)
                     /locus_tag="PKL17_11235"
     CDS             complement(15471..16067)
                     /locus_tag="PKL17_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45351.1"
                     /translation="MLKRAETEFGMLCGELLADLERFRPAVPSATSERDAETALRERA
                     ALRLGELYREFRVDETESNLAEDSQFALYKREVDQVLIPRYARLAQQQNQLEQAPRRA
                     WQGRDLYNRLSWALGFFLVGVFVVWAPFIPIWEKWIPFALAALAPLVSPFLPDLYGAW
                     QNQKHQVALFALHDDLDKLGRALPLPPVSPNKQLGSGS"
     gene            16204..17397
                     /gene="ppk2"
                     /locus_tag="PKL17_11240"
     CDS             16204..17397
                     /gene="ppk2"
                     /locus_tag="PKL17_11240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNK45352.1"
                     /translation="MSTDSPTSPITTATSDGIAVNPESSPRSAAEISATALSSPTNPA
                     TPHHAATLRRTRSGDIAPTESPAAIENFVVETSLDSDREARLSSMRDILSGLDAEESL
                     SLTKLLVRRMRVEGSVKSPNPDDELSKDWRDGGYPYKNLMQRRNYERQKYQLQVELLK
                     LQAWVKETKQKVVILFEGRDAAGKGGTIKRFMEHLNPRGARVVALEKPTDFERGQWYF
                     QRYVSHLPTAGEIVMFDRSWYNRAGVERVMGFCTPQEYAEFMRQVPEFERMLVRNGIH
                     LIKFWFSVSRDEQRRRFKERRVHPLKQWKLSPIDMASLDKWDDYTKAKEAMFFHTDTA
                     DAPWTVIKSDCKKRARLNAMRYVLHKMPYTNKDSQRLGNLDALIVGRAHIVFERGEKH
                     GAPIL"
     gene            17720..>18613
                     /locus_tag="PKL17_11245"
     CDS             17720..>18613
                     /locus_tag="PKL17_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45353.1"
                     /translation="MSHKRRPEFMLPLVALSVLAVADCSPEPAAEPPLASKQQALTLT
                     LTDCLATSLITAITQANTATGPSTITLMGGCTYSLVARNNFYYGPNGLPAITNDITID
                     GTSQGAIIERSAAGATPTFRLFFVAGAGLPPKKAGRLVLQNLTLRGGVAQGGSSSAAV
                     VTGGGGLGAGGAIFAQGEVVLSRVTLTGNSAMGGASAAPGAAACGGGGGMGGNAFGPA
                     GGGFRSATNNNGGGGVAGNEGGRAAAAAAGGAGGTDASGAEPGQPGAAAAGGAGGGTR
                     TLGGGGGAGNVTAAGGTAGDGG"
BASE COUNT         3649 a         5452 c         5697 g         3815 t
ORIGIN      
        1 aacggggttc gcggctcttg gtcaaggtcg gcaagcaggt ttttggcgac atgacgtgcc
       61 tgctggatgg cgacgggact gacgccaggc aagggcttgc cgtcctgatg cagaaacgcc
      121 gccatgtcgc ccactgcaaa cacttcgcgg cgcgctgcca cggatagatc gggattgacg
      181 atgacccgtc cggctcggtc tatttcgccg cccagcttgg cggacagagg cgatccgcgc
      241 acaccggcag cccagagcac ggtcgccgtc gggatgaatt tgccactggc ctcgacgcca
      301 cgaccgtcga ttttggtcac gcgcagcccg gcccggacct cgacacccag gtggcccaga
      361 tcaatcaccg cacgcatcgc caacgcatcg gggaacgagc taaggatgcg gggtccgccc
      421 tcgatcaaaa tgatcttggc ctgggcgggg tttatgttgc gaaagtcgcg tgccagcaca
      481 tagcgtgaca gctctccgag tgcgccggcc agttcgacac ccgttgggcc gccgcccacc
      541 accacaaagg tcagcagccg ctctcgctcg gctggatcga cggtgcgctc ggcggcttca
      601 aacgccaaaa ggacgcgccg gcggacctcg acagcatcgt caatgtcttt gagcccgacg
      661 gcatacttgg cccagtcgtc gtggccaaag tagtgatttt gcgcgcccgc cgcgacgatc
      721 aggtagtcgt accgcagatc gccgcgatcg agcacgaccc gtcgagcatt tacatcaatg
      781 tcttggactt ccgcgagaag tacgctgacg tttggctgga agcgcaccac cgaccgaatc
      841 ggcgcggcaa tgtcagcagg cgaaagaccc gccatcgcga cttgataaag aagcggctga
      901 aacaggtggt gattgcaccg gtcgaccaaa gtcacccgga catttgcccc atcaagctgc
      961 ttggcagccg tcagaccgcc gaagcccccc ccgatgatga cgacatgcgg ctgtgttccg
     1021 ctctttacca ctgggtccaa ggtcgccact gatcctcttc actagagtaa tagaagcgct
     1081 ggttgtcctt ggtgatgacg agcaagtcgc gaccaatggg ccacagcttg tagatgttgg
     1141 cgtcgcgatc gatgtagcgc tgggcggtcg atgaaaacac gcgcccattt aggtcgagcc
     1201 cgtagtagct gtcgccgcga acgaccagct gctgcatcgc gaaggcgacg ggcttcatct
     1261 tgttgtccca ggtgccgttg cgatagcggt atatcttttc gccctcgaac agatacatcg
     1321 acccttggga cgcgacgacg ttgccgcctt tgatgagctt cggccacagc tggatcgaat
     1381 ctttgccgtc tgtcgtcgcg gtgataaggc gtccgtcttc aagaatcgcg tagacggtgc
     1441 cacccgatgc tgcgacctga ctgaccggcg tgatgtgggt cgggcgccag ctcttgccct
     1501 cggcgtcgta gtagatcagg ctgcgcttgt tgtcggtggt aaacagggtg ccagtgatgt
     1561 cgtcgatcgc cacgacgcca aagtcgctgg tcgggaccat ggtgtatttg gcaccgtcgc
     1621 gacggtacgc acggcccttt ttgtcggtga caagcagcgg aaacagctcc gacaccacct
     1681 tggctttggc tgggctgtcc ttggtgatcg ccgagatgag gccgctcacg aactcgatcg
     1741 agtcgaagtc gcgcagtggc acccggcccc gaatggccat cgagttgatg aactcactgt
     1801 ggagcaagag ggcgggccgc ttgtcgagct tctgcgccag cgcattgccg agtgcgatgc
     1861 gggccgcttc aactgcgttg cgcgattcgg cggctggttc tggggtcagg ttggtcgggg
     1921 ccgtcacggc ggaaccgacg ctgccggtaa gcaggctgta caaaagctcc actgggcgag
     1981 cgacgccggg ggtatcgacg gctttgtcgg caaacgtcac aatgccgagc agtcgtccgg
     2041 tcggggacag aagcggacca ccggtgtgat cgagggttag cggcgggtcc gtttcgatga
     2101 tgccggggcg gaccgccatg atcgaaccgc tcatcatggc cagcgtctgt cggccatggg
     2161 tgcgcaggcc aatgacgtcc gcgcccaccg ccgtcgcggt cttcacgtca aacagatcaa
     2221 gcgaccgata ctgctgcttg atcggcggct cggggagctg gagcagcagc aagtgattcg
     2281 gcgcatccat tcgcaccacc tggaccggca gcgctttggc ttggtttgcg cgcaggaaga
     2341 tcagcggatc ttcgtcgcca gcaaacgccg catcgtacaa aaatgcgaag acttggccct
     2401 tttcgacacc agacacaagg cgcgaatcgc tgaccacgac gccgtttggc ttgacgatga
     2461 cgccgagcgc ggtgttaaaa cggctgccgt ctttggcgat gaggaacacc gtcgctggtg
     2521 cggctcgctg atagacatct gacttgctga gcacggggct tttcggcggc agcgcgagcg
     2581 gttttggacc agccggcggc ttcgcggctg caggcgcctt cggtaaaggc ttggctgccg
     2641 gcttggcctt ggctgcaccg ggggtcgcag ctgcgggctg cgcgacggga gcgaccggcg
     2701 cactggctgc tggcggtgcc gccgcgggca aaactggggc cgactgagcc agggccgaac
     2761 cgaccgatgc tgggccacca aacgcaagaa gcaaggacag cgccgacaat gtcagggttt
     2821 ctgcccgccg aaaaaacagg gcagggcgag ccgtcgaggg catacgagca ccgctagaca
     2881 aaacacacct ccgagaaccg aagcagggca aaccgaaaga acactttgcg ataccaccga
     2941 actgtatcgc aaacttcccc ggatccattg gaagaaatgt cgcgtggctg cggggacgcc
     3001 cagacgtcgg tccgctatga tggtgcaagc ggtaaacacg ctgtgggata cacgtcatga
     3061 ccaacctgtt gcgaattgaa tcgcaggcgc ggcgcgagct atcagtttgg cagcgcttct
     3121 ttgcccccga gcttgtgcag acgctggggc cagtggaagc gcagaacgtg cagggcgagc
     3181 tggactctga gctgaaaaag ctgctcgcga tgatgcccga ccccggctgg cgagccccgc
     3241 agatgcgggc gtttggcctg ggcggtgcga tttatctggc gctgtatctt gtgctcgccc
     3301 gacgtggaca gactgccgat caggtctggc atatctgcga gcaagccacg cggacccgct
     3361 tttccagctt gtcgtggttt cagcgcaaga cgatgggctg gcttttgttt tcgccgctgt
     3421 ggaaacagat gacaaagtcc atcgccacga agtcggcgca agctgcggtg gggggctggg
     3481 tgctggacta tgtgccgccc gatccaggtt ccagcgacta cggcgtgacc tatcaccgct
     3541 gcgcaatccg cgatctggcc catgccgtcg gcgccgaagc gtttgcgccg ttcatttgtc
     3601 agtcggatgc ggtgatgagc gagctgcttg gctggggtct gcgccgcacc gggacgattg
     3661 cgcaaggcca aagccagtgc gatttccgtt tccgcaaagg ccagccgacc gacgttcgca
     3721 gcgtcacccg gcttccggtg gtacggtagc catcggctcg catatgaagc atcacaagtg
     3781 cttcatatgg gcgatgcgtt tccggtggtt aggtatagcg cttcatccaa tcgagctggc
     3841 gttcgattct ttcgcggtcg ctgctgcttt gtccgcggga tcgatccccg ccgccgcccg
     3901 agcgattttg cccccgcgat ccacctttgt ccccggactg tggtcggcca tgggggcgcg
     3961 gaccggcccc gcgatcgtcg ggcttgcgtg catgggatgg ctgcggtttc gatccgccgt
     4021 gcggtcgcga cccattgtcg ggccggggcg gacggggacc gccggggctg ccaccactgc
     4081 gctcgccctg cggcctttgc ggctggggtc tcgtgccatg tgacgatggg gggcgtggcc
     4141 cgctttgttc gcgtggctct cgccggggcg ggcgatcgct tggcaccgcg gctggttctc
     4201 ccccgcggtt gctcttcgca aacggatggt ctggtacgac gggaaccttc tgccgcgtga
     4261 gcttttcgat gctgtttagg aagccttgct cgtcccagtc acagaacgca atcgcaatgc
     4321 cttcggcacc agcgcgggcg gtgcggccaa tgcggtgaac gtagctttcc ggttcgtttg
     4381 gcaggtcgta gtttatgacg tggctgacgt tatcgacgtc gatgccacgg gcggcaatgt
     4441 cggtggcgac cagaattcgc gtctcgccgc tcttgaagtg agcgagtgcc gcttcgcgcg
     4501 cattctggga tttgttgcca tgaatcgctt cgccgacgta acccgattcg gcaagctgtt
     4561 tgacgacttt gttggcgccg tgtttggtcc gggtgaacac cagcacgcgc gacaggttgg
     4621 cgtcttttag caggtgcgca agcagcttgg gcttgtcgtt tttggcgact ttgtagaccg
     4681 actgcgagat gcgttctgcc gtcgatgaca ccggagccac tgcgacgcgc tgcggactgc
     4741 gcaaaaacga catcgccagg gcttcaatat cgggcggcat ggtcgctgaa aacagcagcg
     4801 actgcttgac gtcgggcagc gccgcgagca ctttgcgcac gtcgtgaatg aagcccatgt
     4861 cgagcatgcg atcggcttca tccaggacga aaaactgcaa gtctttgagc gagacaaacc
     4921 cctgtcccat caggtcgagc agccgtccgg gcgtcgccac caggatgtcg attcccattc
     4981 gcagccgctg cacctgggca ccttgtttta cgccaccaaa gatcacggtg ctggtgattc
     5041 cggtgtgggc gccgtagacg cggaagctct cctcgatctg gctggccagt tcgcgggttg
     5101 gggacagcac cagggcgcga atcttgcgcg cttgcggggg atgtcgcgcg ggcttgtgct
     5161 cgacgggcgg tggcggctgc cgctgggcgt acagaagctg caaaatgggc agcgcaaacg
     5221 ccgcggtttt gccggttccg gtctgggcac agccgagcag atcgtggcct ttgaggagga
     5281 tcggaatcga ctgggtttga atcggggtgg ggcttgtgta accggactca gcgacagccc
     5341 ggcacagctc tggaatcagc cccaggccag caaagggaga ggtcgatgga tcgaacacag
     5401 gagcgctttc tacacgaaga ggaggcgtca ctccgtggtg tacggagggc tcctttacag
     5461 acattggctt ggtcataaga agggcgcagt ctagccgcaa agcggaaaaa cggcgcacaa
     5521 ttcgtacttt agggaaggat ggcggcgagt cgagcggcgg tgatggggtt cgtttgcaac
     5581 agcgacacca cggcttgttc ttcggcagac agcgtcaccg aagccgaagc agacgtggtc
     5641 gcggcttcgc cgggtgccga tgctgaatca ccggcgggcg caggcgccgc ggcttttttg
     5701 tcccacggaa aagcccaagc agacagggtc tttgtgtcct tgggatacag accgtttacg
     5761 tctttgagca gcttgggcaa aaggtcttcc gcatagggac gcagcgacag cagcagaccc
     5821 ggcagccacg gatacaggat gtcttcgggc agccgcgcaa atgccttgga cagcagttcg
     5881 acgacaacgc ggccaatcac cggggcaaac tggagcgcca gcaaaaaacc cgagacgtat
     5941 tcggggaaca ccggcagcat cagcgggttg tcgagcgtct gcgccaaaaa ctcgcggatg
     6001 gtttcgatgt cgcgcagctt gaggagcagt tccgatgccc acaagaggcc cagcttggcc
     6061 ggatcggtgg tgacggggcc ggcctggcga atcgcgatgt cgagctggct gcgctgacag
     6121 ccgagcgcca gcgcttgcgg ttcgaaatcg aacacaaagc tgagcatgct ggcgacttcg
     6181 tgcggacggg ttccgcggtc ttcgaagcct ttgggcaaaa gcatcgaata gtgcgaatag
     6241 cccgtcgaga cgaagtcttt gagccacttg ggaagcccgg ctggcgtcgc gcgatagtag
     6301 tggaccaggc gacgaatccg ctcgacgact ttgggcgcat cctgcgcgct catctcattg
     6361 cggagaagat caacggcgcg gtcgcccagc tcttcggtaa gtcgctcgct cttcaaaaac
     6421 aggatcgagt cttcggcggc tttgagcgca gtaaccgtct tggcctgggg tccaaaggct
     6481 tcggtcttga gccgtttttc gacgacgtat tcgaccgtga caccttcata cccgagccga
     6541 atgatcgact cttggtattt gccgatcgaa acatcccacg actcctgcac cggcacatgt
     6601 cccagcttgc gctcgcccat gatgggccgc agcaccggat gtccctgggt caagtagtgc
     6661 agcatccaca gaagatccga tgccggcaaa agatgcggat cttttttgaa gtcgagcagt
     6721 gcccgctgga tctgcgacgc ttcgaggttt acgccgagcg gtttcagccg ggcgtagaca
     6781 tcctgcgcga gcggtggcag tgccgaataa ccgacgcgcc ccgcacgatt tcccccgagc
     6841 agcatctgac agagcttggg tacgttgcgc tttttgacct ggcgatcttt ttccagacag
     6901 gtgatggccg cgtcttcaaa gtcatacggc gagggatgct gccggttgcg caggttggca
     6961 agcagcatcg acgtctggta aatcgcaatc gcatctgccg tcgacgtgag atacccattg
     7021 tcgcgagcga gcgaaacgat gtcgaccgac cagcgcaaaa gctgttcttc atcggcgctg
     7081 tgcagaagcg gtggcttcac aaagaaaccg agcacgcgag cagtatcggc cttgccaatt
     7141 ggggccggtg cagaaaccgc gggcgcagct ttcgctttgc tcgctttgcc agaacttgtg
     7201 gcagggctgc tggctttttt cgacagcttc cacggcttca cagacagcgc cgcgatgcct
     7261 ttttcccagg tggtttccga aagtgcaacc gtaccggcgg gatgatggaa ctgatgctca
     7321 atcgcgatgt agctcgacgg gatcaggccg tacaaccact tggtgggagt cgctttggga
     7381 atgtcccaaa ccgcaggcga atgggtgcca aactcgggga cttcgctgac ggcgtgcacc
     7441 gcaccacaga gatagatcgc atcggaagga gcgattccgg tctttttcag gtgctccttc
     7501 atgcgtgtcc acatgtagcg ttcgcggcgc ttgtcgtttt cgtgatcttc gtctttgcga
     7561 cccaagcgac gcagcagact tccgaccagc gaaagcacaa agcgatacgt cggatagtct
     7621 gccgccagaa cggcttgctc gacgtactga tcccaccact cggtgaagtg acggacgcgc
     7681 gcgtttttca aaaggaatga caggaagaca tcgaaggtcg gttcgacgtt tcccatttgc
     7741 agaccaatcg ccgcaccgtg gtcgggaaca tcgctgtcct cttcctctcc tggtgctttg
     7801 ggggtcgctt ggtcgcgctc ttcctctttg accatgtgct tttcgagctc gtcttccttt
     7861 tgcggcatcc actggaagac gtaatcgaca gcgcgatcga caaagaccag ctcggtgctg
     7921 ggattttcca gcgcatacgc gatggcttgg aactcggcgg aaaactcggt cagcggcgcg
     7981 accaccgaca gcggcgacca ccctttggga aacgcatcgg cagtctgggc aaatgcttgc
     8041 agagccaccg gcaagcggca gtcccgcagc ccttcgatcg tcccgcggag gtcttcacac
     8101 atctccatga agatcacttt gggaggccgc tctgccagcc gcttggcaac gtggagcgcg
     8161 gaagacggag agtggtgaca gaccggaatg atctcgagcg gctctcgcat cgcgcgatcc
     8221 acatcggaga ccatcaggcc gagcagctct cccactttgt gcttgtcggt cgcaaactct
     8281 tggaccgccg cttcaagctg ctcacgcaga gccgccagcg cttcagcatt cgtcgctggc
     8341 agggtagagg aaggagtccg ttccatacgc aagttccttg ctgtgatcgc gcgctatcgt
     8401 cactacttta gaaggctcat cgccagcttg ccgccttcca ggtactcttc ccactcgccc
     8461 ttcttttcct tggcgcgctt gtccacgacc gagtgccaga acttgttcat caccgacact
     8521 tcttcaggaa tccggcggac cagggttccg accagggctt tggccagatt tccagcgctg
     8581 agcgtggttt ggccaaaaaa ctggctgtgc agaacggcgt cttccagcac gccaatctgt
     8641 tccgcggtgg aaagggaaga ctccagccgc tgatcatcgc tcgtcgctgc ggtctgcgca
     8701 tcacgcagat ccgaaaacgt ctgcagcaga atgtcgagaa gcgcgggtgg cagctccact
     8761 tcaaactgat tgcgggttaa aagctcacgg gtccggaaca gtacgatctc tttttcgctc
     8821 ttcttgttgg tgaccgtggg aatcgtcaca aagttgaagc gacgcttcag tgccgtcgac
     8881 agatcgttga caccgcggtc acggctgttg gcggtggcga tgatgttaaa gcccggacgc
     8941 gcaaacacaa cattgtcgcc agagagctcc ggaatcgtaa tgtacttttc cgaaaggatg
     9001 gagatcagcg catcctgcac atcggacgtg cagcgggtaa gctcttcgat gcggcccatt
     9061 ccggcgtgct gcatcgcgat catgatgggg gacggaatca gcgattcttt ggactgaccc
     9121 ttggcgatga ccagcgcgac gttccacgaa tacttgatct gatcttcggt cgttcccgcg
     9181 gtgccctgca cagtatgtgt cgagctgccg ctgatggccg ctgcgagcag ttctgcaagc
     9241 cagcttttgc ccgtgccggg gtcgccaatc aaaaggagtc cgcgatcgga agccagggtc
     9301 acgatggcac gttcgaccaa cgctggatcg ccgaaaaact tctgcgcaat tggacgctcg
     9361 atcttgtcgg ctggcgtcga tccaaggata aacgtccgga tcatgcgcgg cgacagcttc
     9421 cagctaaacg gacgcttgcc ggtatcaatc gaagccagat aggccagttc ttctgcgtac
     9481 ttgacttccg cgggctggcg caggacttcg gctttctttg ctttgggagt ctctttgact
     9541 tccgctttca cttcgaccgt gaccgaagca gaggccgtgg cttccgcttt ggtcgcaatc
     9601 ggaaactcgg cgctggcgga cgtgctggtt tccgcagcgg caggagcggg cgccgcggaa
     9661 tcggttgcag gagtgccagc gggggcggct gcggcagcgc tgtcatcggt tttggtcttc
     9721 ttggtacggg gagtcgccat gtcagagtct cctaattgag caggtagcga agctggtgaa
     9781 ttagcttgtt gacggcgcca tgcaggattg gcattccgag ttcagcgagc cgcgtgcgga
     9841 aaaactcgct gaccgaaaag tagccactcg actggagcgc gccgacggga atgaagtgaa
     9901 ctccagagtc cttgagtcct ttgatgaaat cgagcagttc ctgatccgag ccgccttcgt
     9961 aaaagtccga gatgagcacc atggcggtgt tcttcggctc gacgattttc ttggccgcct
    10021 cgatcagcgc gagtcggatg tacgttccgc cgccaagctc ggtccgcaaa aggacttcaa
    10081 acggatcatg aacccatggg gtcagatcga gtacgcgggt atcaaacgca accaggtgga
    10141 cttccacttt gggaagaccg gcaaagatcg acgccaggat cgtgctctgc accatcgcat
    10201 cgaccatcga accggactga tcgaccacga caatcagtcg cgtcggggtg gttttgttgg
    10261 cggtgcggcg atacgtcagt cggtcgacat acagccgctg ctcgtcggga ttccagttgg
    10321 tcaggttttt ccacagcgtc tttttcagat cgagattccg aaacacgcgc ttgggtggaa
    10381 tgctgcgatc gagcttgcct ttgctcgcct tctggacttg cagtttcagc acgtcagcaa
    10441 gctgatcgac atacgcgcgg atcagcttct tggcattttc cagcgccaca ccggacaggt
    10501 tggctttgtc ggccagaagc tgttccacaa gtgcaatgga cggcgacagc ttgcttgcca
    10561 ggcgcgagtc tttgagaacc tcgcgcagct ccatccggcg caccagctcg ctttccagat
    10621 tctgaaatcc gcggcgcaga tcgtcttccg aaaggagtct gcgtccaccg cctgggcccg
    10681 ttcccccggc gccacctgca ccaccgccac cgcgtccacg cagaccgcct tgcggataac
    10741 caaacgcttc ttccagtcgt cccaagtctt gcgtccactg cgagtactgc tgcgcagtca
    10801 cggtcttggt ctgggtctgc ggaccaaatg cattcagcag cagcttggaa agcaccagcg
    10861 tgcggcgcag tcgatccttg tcgcttgcag agtctgcggc tgcattccca gatccatctg
    10921 gattcgcact ggtcagcgcg acaccacctg gactttcatc gtcgagcatc gcgcccggaa
    10981 gtggggtttc aaagtctttg cgaagttccg gaaagcgctg cgtaagcgtc gtgatcgaca
    11041 ccattggatc gagcacaaga tcaggaacgt cgacttcctt ggcgatctct ttgctcagcg
    11101 cttccaggtt ctgcgctttt tccccctgac caaagcacgc ggcgaccaga cgccacagaa
    11161 gtgcctgctg ccgattctgg agatcaacgc ttggcgtggg cgaagcgggt tttgtcgtat
    11221 ccgtcatggg attccttctg cgtccttttc gccttacgac ttcttgcgca ggagcttgcc
    11281 ggcgcgctca cgcagcaccg caacggtgtc atctttagat ttgagcgact gcggtgggac
    11341 tccggccagc tcgacctggc ttccgatctt gatggcgagc ggctgcaccg accaccgtcc
    11401 ccgatcgaag cggagcaggc caacgagcgc actcgctttt gcgatccgct ccatcgtcat
    11461 tcccgagctg tccgataggc gcgcccccga cagcggaagc ttggttccgg aaacctctag
    11521 ggcttgttct tcgacaagct ctgctgcgga cccgcgctta gacttgtggg actcgatctt
    11581 gactgtctta aagtcgcgca gaaagaaaag ctctgcaagc tgcgcagggt gacgatccaa
    11641 accgctcagg ctgggcctgg taagcttcgc agaaggagcc aagaacgact ctgcaatgtc
    11701 gaagggagag taggatggtc cgagggcagc gcggctttca tcccacagca aatcgccggt
    11761 tcccagcatt tgcatgtcag tcagcttgat catcgcgcag tcgcgaattc ccttgagcag
    11821 cttgtcgtag ctgccttcca gaagtcgcca catctcgtgc gcgatgacgg tatcaacttt
    11881 gtatgcagac accgtagttc gcacgtaacg cacgtcgcta ccgacacgca gtagaccatg
    11941 cagacagacc gaaaacaggt tgcgatggtg atgcagatcg cagccgagtg gcagcagttc
    12001 tccgctgact gtttcgatct tggcggcggg ctgcacagac aaagaaagca gcatcgcgcg
    12061 cgaccacaga tcgacccagc gatatgtcgg aaccgtcttg cccgtgggaa tcggaagcgc
    12121 cgaaaggagc tcggatagga atcccgacag aatcgcagaa agacggatca gcgcgggctc
    12181 ttcctgaatc ttgtcgagcg ttgcataaaa cggatacaga gactcgggtt caagctgtcc
    12241 aaagccggtc agcgcgatct ccatcaggaa gtgacgcgcg ctttcacgga acacaccgtg
    12301 gtggccgggc gattccgggg tcgagagact cggttcgccc aggttgggag tccgtccaag
    12361 cgccgtactt gcctgtgcag acagtgaatc agccatcgcg ccttgcagtg cagcgcgcgc
    12421 gcaggccagt gtggcgaagt gtttttcgac aaactcactg cgcaaaaggg cgtcgaccgc
    12481 ttctttaacg cggggaccga gcggcgtcgc agcgagcaag cggggcaggg cagacaccgc
    12541 tgcgacttcc cgttcgctca tccggccaaa gccggtaaaa aaacagtgat caagacgcgc
    12601 gatgacctcg gcggtttcag cgacaccggg tggtagcgtc gccagcgcgg taagggtatg
    12661 gctcatgcga agtcctttgc ggattcccga agtgcgtatc gattagcccg agacaaacca
    12721 cgtcatctcg ggaatcggct gattggaaga agcaagctcg aagtatcgca ggtgcgagag
    12781 gaaacgcgaa aacacctggc tgacatccag acgtgactgc acgccgcgga cctgcaacat
    12841 gatgtcgcgc gcagaggtga actttgacag atcaccagag agcttcagat agcgagccac
    12901 cgtctctgca ccaaactgaa gcacggcttc cgacatcagc tgcgctaggt gtttgcaagg
    12961 cgtgccgcga cagccactgc acggacggtt gttgttggtg gaacagttga agttcacgct
    13021 gctgccggct tcgaaaaacg acacatagac gcggttttca tccgatccag aggacaccac
    13081 accttgcacg cgcccgccga acagctcgac gtagggaacg tctttcactt tgcgcggacg
    13141 gctcatcggt tcggacaaag gcagcgatgt gatttcagga gggagtagcg ccatgttggt
    13201 attacgctcc gcggtgtcgc caaaggaatg tctgttgaac ttgtgcaaaa gggtctgcat
    13261 gccctggtag gacatcgtgc gcagcaccaa gacacaaaga agcgtgggaa tcttacttta
    13321 caaccgctag gttgggaagc ggttttttgc ggcctacggg actattcgga ttcgtgtgca
    13381 gaggacgaac cggaagtgga ttcttttgcg atcacagcct cgacaattcg cgtgacatcg
    13441 agcttctgga tgttggttcc agtcgccgca gggacaatca agatcggctt gccagccagt
    13501 gcatcggcga tgcggagctt gactgcagta cgaccacctg cgccggacat ggctttgttc
    13561 tgtttttcga tcgctttggc ccgtgcggtc gcttccgcaa ggagtgcttt tgccgtgcgc
    13621 tcgttttcat acaaggcgcg atcggcacca atccgaatcc gctctacttt tcctttggct
    13681 tcggcaatct gtgcctgcac atcaccgcgg gctttttcca gctgacgcgc ctgctctgcc
    13741 tcggctgcct gcgcttcact cttgagcctt tcggagttct gttccgcgat cttccgttcg
    13801 tgaatgaccc gttcatattc tgggttaaag cgctgctctc cgggcatcac gtcttcgatg
    13861 atcacgccct ccgatgtgag ctcttccgtc agcttggtct tggctttttg ggccttttcg
    13921 agcagctgtt tgcgttcaaa aaactcttcg gaatgaagct cattgaagga atcacgcacg
    13981 taggcgcgac acgcgggacg aaccagttct tctttgagca gcgctgtgct gcgtgcgcca
    14041 cgagccagga tggctggggc tcgctgtgga tcgattcgcc acgtcacagt gacatcaatg
    14101 ctgatgtcat tcccatcgcg gctcttgagc acgatggcat catcgccttc gcgatccccc
    14161 cgttccttcg ctgcggtcat ttccagcttc tgggtcatga tgtcaaacgt cgcccagtcc
    14221 gacatccacg gaaagaacat gtacgtcgcg ccaggttcgt agatgtgcgg ttcgatgcca
    14281 gatccaaaca gcttgcgcgt tcgtacacca acctctgtgc tctcggtggt gtaggtcgtg
    14341 caacccagga ctgccaaaag cggcagcaca gacagaatgc ggactacgtt cgtaggaagg
    14401 aatcgcatta cttcacctcg aactggcgca gaagttcact gatgttgagc ggatttgtac
    14461 ttccttgtcc cgtcgaagga acgatgatca cgcggactcc tttcatcagg tcggcgagct
    14521 tctgccccac catggcctgt gctccggctc cttcgagcgc gtgattttcc atttcggttc
    14581 cttgtgcctg cgctgacttc accagcaggt cgccttctgc tgctttggtg cgctgataca
    14641 gatttgcatc ggcgataagc tgcgcgattt cccgcgaccc cttttcacgt tccacttgca
    14701 ccgcggcttg gccggtcgaa atgacttcgc gcttgtgggc atcctcacgc gcggcggcgc
    14761 tctctgcacg gttttttagg accgtctgat cttggatttt ccgctgctcg atctggtgct
    14821 gatacgcctt gtcataggaa aattggcgga tcaggatttt caccagttcg attcctttgt
    14881 cggcaaactc tgttcgcagc atttccgtcg ctgcttgcgt ttgtttcagc cgcttgtcac
    14941 caagataaaa ttcctccgca gtcagctcgc ccagcgcttt gcgcagcgca ttgtccgaac
    15001 gcggaatcac caccacgctt tcaaacatcc gacccggccc gatcgttgtg atgactcgat
    15061 atggatcatg gatgcgatac agcaccgtgg catccaccga caccttgtag ttgtcggcgg
    15121 tctgaatcac gatcggtccg tcgctgcgtg agctcttgga actctctgtc ggattggcgc
    15181 tccggttgag cgcttgcaga tcggtgggga agatcagcac ccgttcatag ttcggaatca
    15241 caacatgcgt tccggtttgc cagggttcct tttggattcc tgactttgat ccaaacgaga
    15301 tggcgtggat tccgacttca tttggtccga tgtacacagt gcagcaggaa ctgagccacc
    15361 agcctcccag aaacagaatc agtggaatgc gaatccagcg ctgactgagc aagtacttga
    15421 cgacgtttac gagcgtctcc atgcagactc ctgcgcgaaa gggggccctg ttaggaacca
    15481 ctcccaagtt gtttgttcgg actcaccgga ggcaaaggaa gcgcacgtcc gagcttgtcg
    15541 agatcatcgt gcagtgcaaa cagcgccact tgatgtttct ggttctgcca cgcgccatac
    15601 agatccggca gaaagggact cacaaggggc gcaagtgccg cgagtgcaaa gggaatccac
    15661 ttttcccaga tcggaataaa cggagcccaa actacgaaaa caccgacgag aaaaaagccg
    15721 agtgcccagc tgagccggtt gtacagatcg cgtccttgcc aggcacggcg cggtgcctgt
    15781 tcaagctgat tctgctgctg cgcaagacgt gcatagcgcg gtatcagcac ctgatcgact
    15841 tcacgcttgt acagtgcgaa ctgggaatct tccgcaaggt tagattctgt ctcgtccaca
    15901 cgaaactcgc ggtacagctc accgagtcgc agtgctgcac gttcgcgcag cgcggtctcc
    15961 gcatcacgct cgctcgtcgc tgatggaact gccggacgga accgttcaag atccgccaga
    16021 agctctccgc agagcatccc aaactcagtc tcagcacgct ttaacacggc agtctggcca
    16081 gattttaccg cctgttccga gcgcgtggcg gaaaattacg ttgcgtgatg tagaagcggc
    16141 cccctgcgcg ggcgcatgtg ggtatgatga acccgcaaca cgtattggca ctgtgaggag
    16201 atcatgtcca ccgattcccc aacgtccccc attaccaccg cgacatccga cggaatcgcg
    16261 gtgaatccgg agtcttcccc ccgttcagcc gccgagattt ctgcgacggc gctgtcatcc
    16321 ccgacgaacc ctgcgacacc gcatcacgcg gccacgctgc gccgcacaag aagcggcgac
    16381 atcgcgccca ctgaaagtcc cgctgcgatc gaaaacttcg ttgtcgagac ctcgctggat
    16441 tccgatcgcg aggcgcggct gtcgagcatg cgcgacattc tgtctgggct cgatgcagag
    16501 gagtcgcttt cacttaccaa gctgctggtg cgaaggatgc gcgtcgaagg atcggtgaag
    16561 agtccgaatc ccgacgatga gctgtcgaaa gactggcgcg atggcggcta tccgtacaaa
    16621 aacctgatgc agcgtcgcaa ctacgagcgg caaaagtatc agctccaagt cgagcttctg
    16681 aagctgcaag cctgggtgaa ggaaaccaag caaaaagtcg tgatcctgtt tgaaggacgc
    16741 gacgccgccg gcaagggcgg aaccatcaag cgatttatgg agcacctcaa tcctcgtggc
    16801 gcgcgcgtcg tcgcactgga aaaaccgacc gattttgaac gcggccagtg gtacttccag
    16861 cgctacgtca gccacctgcc caccgctggc gagatcgtaa tgttcgaccg atcttggtat
    16921 aaccgggccg gcgtcgagcg cgtcatgggt ttttgcaccc cgcaggagta cgcggagttt
    16981 atgcggcaag tgccggagtt tgagcggatg ctggtccgca acggcattca ccttatcaag
    17041 ttctggtttt cggtaagccg cgacgagcaa cgacggcgat tcaaggaacg gcgcgtccac
    17101 ccgctgaagc agtggaagct gtcgccgatt gacatggcgt cgctcgacaa gtgggacgac
    17161 tacaccaaag ccaaggaagc gatgttcttc cataccgaca ccgcggacgc tccgtggacg
    17221 gtgataaagt cggactgcaa aaaacgcgcg cgtctgaatg ccatgcgcta cgtgctgcac
    17281 aagatgccgt acaccaacaa agactcgcag cggcttggca acctggatgc gctgatcgtt
    17341 gggcgtgccc acatcgtgtt cgagcgcggt gaaaagcacg gcgcgccgat tctgtagtgt
    17401 ggttcacctt ccgccggcct gtcctgcgcc ggcatctgcg tccgtaagac acctgtcttt
    17461 ttagccgtac atcgaagaat cgctgatccc ccgaaacgag ccacgggaga ccgggacacg
    17521 ttatttcccg tcccctaccg ttgcttgttc gatgccagtg agacaagcca gcgttttgag
    17581 ataaaatcgt accaatttag taattcaata cacgaattta ctcatgtccg gtcctcacca
    17641 cgacgctcgg agcggtcatc ttgctggctg caaggctgcg ccgtcgcggc ggctggggtc
    17701 ggcacaggag cttgcgaaca tgtcccacaa acgtcgtccc gagtttatgc tgccgctcgt
    17761 cgcgctgtcg gttttggcag tggctgattg cagtcccgaa ccagccgccg aaccaccgct
    17821 tgcgagcaag cagcaagccc tgacgctgac gctgaccgac tgtctggcca ccagcttgat
    17881 cacggcgatt acccaagcga atacagcgac gggaccgagc acgattacgc tgatgggtgg
    17941 ctgcacctac tcgctcgtgg ctcgcaacaa cttttattac ggcccaaacg gtctgccggc
    18001 gatcacaaac gacatcacca tcgacggaac cagccaaggc gcgatcatcg aacgttccgc
    18061 ggcgggtgcc acaccgactt tccgtctgtt tttcgttgct ggcgctggct tgccaccgaa
    18121 aaaagcggga cggctggtgc tgcaaaacct gacgctgcgt ggcggcgttg cgcaaggcgg
    18181 aagcagcagc gcggcagtgg tgactggcgg gggcgggctc ggggctggtg gcgcaatctt
    18241 tgcgcagggc gaggtggtgt taagccgcgt tacgctgact ggcaacagtg cgatgggcgg
    18301 ggcgagcgcg gcgccggggg ccgcggcttg tggcggcggc ggtggaatgg gcggaaacgc
    18361 atttggtcca gccggaggcg ggttccgcag tgcgaccaac aacaacggcg gtggcggtgt
    18421 tgccggcaat gaaggcgggc gagcggcagc cgcagcggca ggtggcgccg ggggaacaga
    18481 tgcatccggg gccgaaccgg gccagccagg cgcggcggcg gcaggtggcg cgggcggcgg
    18541 aacaaggacg ctcggtggcg gcggcggggc cggcaatgtc acggcagccg gtgggaccgc
    18601 gggcgatggt ggc
//
LOCUS       DAORGN010000088        18445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1214_length_18445_cov_5.336542, whole genome shotgun sequence.
ACCESSION   DAORGN010000088 DAORGN010000000
VERSION     DAORGN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18445
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_18445_cov_5.336542"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="PKL17_11250"
     CDS             <1..1947
                     /locus_tag="PKL17_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK45354.1"
                     /translation="LEEVWPTVDAWLCHRDRGGKRPAQIGRLLTQTPMQDSSSRARDL
                     RLLSAGPRALWLKTGRMALDVSKGTRWLRWQLPRVMQLVRFWDSATMSIGATLAQQAQ
                     TIPDRTFLLWEGRAYSYADADRRVSAMAGALYACGIRSGHTVGLLMDNHPDCLTALVA
                     CSRIGAIPALLNPATRGAALAHALQVTQVRDLISLASAPDLTQTYGVRRLILGGGPRD
                     PLQSSQQVVDLETIAADLPSTLVQNPGHASDIAWLMFTSGTTGKPKAVKISNRRWLMA
                     ATAAATGCDLTQADTVYCCLPLHHGTGLLLAAGGALLGGARLAIAPRFSVRGFWEDVR
                     RTGATVVFYVGELCRYLVSAPPSPLERSHAVRLFVGNGMRPDVWRELLRRFGQVSVLE
                     FYAATEGNAVMTNLTGEKIGSVGRVPLGLVRTLVVKYDPDSETYPRTADGFLIPAAVD
                     EPGMLLIEIQNRNPLSRFDGYTDSRATEAKILRNVLRQGDAWFCSGDLMREDADGDFW
                     FVDRVGDTFRWKGENVSTEQVAAVLSELSFVEMCAVYGVRLPGQEGRAGMAAIKLRPE
                     QHLDPEALFAAVDQHLIDAARPRFVRIVSELQTTETLKIVKQPLLADGFDVSRTGPLY
                     VYDKPRRTYVKAAADELSALLPTL"
     gene            2138..>2708
                     /locus_tag="PKL17_11255"
     CDS             2138..>2708
                     /locus_tag="PKL17_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK45355.1"
                     /translation="MSAPRTAAVKAAYSTMARLCQSVGQVVHGKSEVIRLAVVSLAAR
                     GHILLEDIPGVGKTTLARALARATGGTFRRIQFTSDLLPSDVIGVSVWNEESRQFDFK
                     HGPLFANVVLADEINRAAPRTQSSLLEAMSEGRVSVDHYTYTIEQPFLVMATQNPLEH
                     FGTYPLPESQMDRFLLRLRLGYPSRYDERR"
     assembly_gap    2709..2735
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2736..3116
                     /locus_tag="PKL17_11260"
     CDS             <2736..3116
                     /locus_tag="PKL17_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK45356.1"
                     /translation="VEDMKPAVAPAEVVQLQDTVCMVRVEESLLEYAERVVAETRRSP
                     LLQLGVSPRGFQSWFRAAQARALSENREYCVPDDFRELALPALAHRLVLPGSSQGGYS
                     DALSNGRAEAERILTGIIERVPVP"
     gene            3113..4210
                     /locus_tag="PKL17_11265"
     CDS             3113..4210
                     /locus_tag="PKL17_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNK45357.1"
                     /translation="MSLRFITRLRGVRLTRDGWWFCTAGLCLFFVALNSGNNLLYLLL
                     SMLIALMAVSLVLAEASLRGVIPRRQPPPRLHAGSPFLMGIALRNDKKQLPSFSIEVE
                     DLCDDKVVDKRCYFLKIPSGRTQQTAYRHTLPRRGRYVFSAFRISTKFPFALFRSSRY
                     IEHRSELLVYPALIPLEHWPKALTSAVGEQSVQRLGRRGNFHALREFRQGDDPRDIHW
                     RSSARRQRLMVREHEIEAVQQVTLYIDNSLPTDKLNLADEQAKLEKVISWGASLGAFY
                     LDHGYAVRLCVRSEEPPLPLLWSPASLQKLLTTLALLPTVSPDVPFQKPPPLPRHAAV
                     PVAEHGDSLVVIRPGAPIPREKHMGHVVEAS"
     gene            4207..6339
                     /locus_tag="PKL17_11270"
     CDS             4207..6339
                     /locus_tag="PKL17_11270"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNK45358.1"
                     /translation="MRFAATHKVVSYLMVTTAFLMLALTGELPPWLLLLTLAAMGGSY
                     FFDPQQHPLMLRRSYNYSLYGAMLLLSALLLGNASGPEALWDGGLRMLCVLLVGKLWQ
                     RRHNGDYLQVYVVSFVMLLGATFVESSMLYALLLFLYVVFAMWTLTLFHLRREMEENY
                     LLKHLPGRHGQAAESERVEVERILNSRRVVGPPFLLTTALAGLCTFFVALGLFLLLPR
                     FHVTVELPFHRRALPSTGFSERLELGSHGLLRDNPRVVMRVELPGQNPPSVLRLRGVS
                     FARYEQGRWSQHRLGEQPLGVSQGLAQIDAVRDLHQPPLRRTEIYLEPLDAEALFTPT
                     PGYPVALYLPDAFAAISGLLTVTSDGQLAARGRRAPLHYAVLSSSESPSLPTDDLAIY
                     RELSSDLRLRLRPLALQMVGDATQPSVQAERILAYLQKNYSYTTRLWKPSEREPIFEF
                     LQRERRGHCEYFATAMAVLLRSLDIPSRTVNGYLSPEWNRYGRFFVVRQQHAHSWVEA
                     LLPGQGWVVLDPTPSQSPPAPWPILHPLRQLFDGLDMSFGKYVLEYDASAQQHLGERL
                     LRLWQKRAASKQIDPEPPSFTYKKLLRSALLLLSSGLFAWLLYRRLKRPSDELLAGER
                     EGRGLLVQALTVLRRRGFVRGPGETLRKLSERISDDGDPIAPHFSQLVEHYYAHRFGD
                     QPLDRALVDRLIDQMRATPRRPRPEMRN"
     gene            complement(6336..8012)
                     /gene="ettA"
                     /locus_tag="PKL17_11275"
     CDS             complement(6336..8012)
                     /gene="ettA"
                     /locus_tag="PKL17_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNK45359.1"
                     /translation="MAAQYIFTMQNLRKVVPPQREILKGIWLSFLPGAKIGVIGSNGA
                     GKSTLLRIMAGVDHEFQGEARPADGIRVGYLPQEPQLDPSKNVFENVAIAVKDRLDLL
                     KRFEELSMRLGEDLPAAEMDKLIEEHSRVQDEIEAKGAWDTERQLEIAMDALRLPPPD
                     QDVTVLSGGERRRVALCRVLLERPDMLLLDEPTNHLDSDSVFWLERFLAEFPGTVVAI
                     THDRYFLDNVAGWILELDRGQGIPWQGNYSSWLDQKQKRLSVEEKQESARQKTLAREL
                     EWVRQSPKARQAKSKARLAAYEDLLKQSQEARIEVAEISIPPAPRLGDIVVVAEGVQK
                     AYGDRLLFDDLSFRLPRGGIVGVIGPNGAGKTTLFRMIVGQEKPDKGKLVVGETVKLA
                     YVDQSRDALKGDKTVWEEISGGVENFEMGKRKLQSRAYVGQFGFKGQDQQKRVKDLSG
                     GERNRVHLAKMLRAGGNLLLLDEPTNDLDVDTLRALEEALLSFAGCAVVISHDRWFLD
                     RIATHMLAFEGDSKVVWFEGNCRDYEQDRRRRLGKDADQPHRIKYKKLTR"
     gene            complement(8043..8789)
                     /locus_tag="PKL17_11280"
     CDS             complement(8043..8789)
                     /locus_tag="PKL17_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK45360.1"
                     /translation="MRAVVKSEPETQSGSTAALAVAESTPDVGERLGSIGTATTEPMD
                     GRVARAQRLRHERSTQLLSVARRLFAERGYHKTSIQDILDTAGVARGTLYLHFDGKRA
                     MFDALVDAFLTSIQSVVTLVDVSPDSPPPLVQIEANLRRVMSVLSENRDMTRIVLLMA
                     EGLDSDADDKMADFYERVRLLLKGALVKGCAMGLFGPIDPEIVSQAALGGLKEVALQW
                     IVRRDSDEKNLHRVCREILSYSLSGMLART"
     gene            9091..9297
                     /locus_tag="PKL17_11285"
     CDS             9091..9297
                     /locus_tag="PKL17_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HNK45361.1"
                     /translation="MAGSDKRKQSLYFPEDMLKEIQEEATRQDRSLSWIVQKAWKIAR
                     NEIRRYPSVNEIEGGEDDERGSKE"
     gene            9251..10213
                     /locus_tag="PKL17_11290"
     CDS             9251..10213
                     /locus_tag="PKL17_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK45362.1"
                     /translation="MKSREEKTTSGAARNSDDPKRDTSSRGRGKRRSGFAISRDGAAI
                     FFEVLGQPHPAPTLVLCDGLGCDGYVWKYLQWALADKWRIVHLHYRGHGQTPLPKDFG
                     QVSIADFATDVLAVMDASETERAVICGHSLGVQVCLEVYRQGRERTDGLVLICGSYRN
                     PLSTFKGKETLGDVLPILQAAVSRVPRLVGAFWKRLLPLDLSYQIARRFELNGELVHR
                     EDFAPYLDALSRMDPRMFVATMAAAGQHDAQDVLPQISVPTLLVTARHDTFTPMKLSE
                     EMREKIPGSELHVVEAGTHTAPIERPSEVSQVVRSFLERRILGQ"
     gene            10210..10812
                     /locus_tag="PKL17_11295"
     CDS             10210..10812
                     /locus_tag="PKL17_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45363.1"
                     /translation="MNDPQSQSETAPDGALTPRIHWVNHVLLWFMRVGIVTMIIVTGV
                     YMYLRDKPSPEQEEMIRYVEVNIPALEYHEAPILSQLDALLRDKSLRPESARKQLADE
                     LMPALVRLRKVAEGPVSVAKTPPVKQLAEEYRQSVEELINVCRTTIRVIDDPKLGERE
                     GYQQVLDSLRRAIAQNRTWRDHVAETTHRLQLGQRGLHKK"
     gene            complement(10819..13125)
                     /locus_tag="PKL17_11300"
     CDS             complement(10819..13125)
                     /locus_tag="PKL17_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK45364.1"
                     /translation="MTDAANSASIPASGSDSYAAQARGDDDAYASYYAGMDKSMQQKV
                     ALTTAFFPPSGILVDMGCGSGSGSFDLASLFSGLRVIGVDIAAQAVEYARAHYQRANL
                     CYQQGDIADPLFAPGSVDGILNSSVWHHLTSFNGFSHREVERALRHHAEALRPGGVLV
                     VRDFVVPRFPTEVWIDLPSDDGTSDGAVTGLSTAALFHRFCAQFRSSQNPAGPVPYQQ
                     LPSPQPGWSRFLVAGRAAAEFLLHKDYRSDWDAECREEYTFYSQAEYEAAFRRHGLRI
                     VLSVELHNPWIIENRFRGRCALHQISGEPLPFPPTNYLIVGEKVAPHHGVRLAVDHAP
                     LATPHFLRLHTLRHQKTGQLFQLAERPNPVLDVIPWFRHGGRVLVLAKHGFPRPLSTT
                     LLDGPALDGAHVAGYLTETITAQVRDLPFAHSDKASATILATRAGLPNEAIAAIQSGL
                     GYYTSPGGLSEHVSTRLCELDVSKLPQHSDGFPKFSDEVQNYTPFTTAGRVQPFVAAQ
                     LLRAAQVGGLQDARLEISVYHLLLSLGEALGPWIGIELSLSPQTAPGFVPAITADVLA
                     TRREPAFAQVDSSDSAHGFLSLYKATVEELDASDKVLRRTELELCAPRSFGLSTVSVI
                     PVVRLGNQYLVGLERRDLPAVQLHTAGQHSTLLTCPAWRLPKTIQTFPDAEALVLRNL
                     LAHFGVKARRVFPLGGKYYPSIGATPEVVYPLVAEVAAETLASSALAFVPLDELVAQR
                     QHIEDGHLLCAMFRLAHALSITAPSPAT"
     gene            13405..15393
                     /locus_tag="PKL17_11305"
     CDS             13405..15393
                     /locus_tag="PKL17_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45365.1"
                     /translation="MIVENQAKLASLAMTHHLTLRRESRASMFSSVAARVRPIEAGHR
                     VWEHLAQEARAVGADLVAVVDEGGALLAQDGAGAIALAEAAARSGIAPSGALLVMGAQ
                     IVEAFRIPVAYDGKQAFLIAACRILPESLRQDAEPLGLGVAIALEGSFVTNLPADALS
                     SGRWGDDPRESLHNLSVRYHLRATPLWQGRLLVAVPLQSVQQWSRDQLIGSLLLVGLL
                     LGGVLLLALWVQLGVMSPLLRMSGAASRVSQGSLARGRAQLKPFLDRNDELATIGKGL
                     DWAVQRLQSLLHTSNRVLDDIDSTIATIERSASTLATSSGTQEERSKEVQATVVPITR
                     AIERLTRQMVEVRNAVVQISLAWSNADQAQAQVLSAARRADSMLHNPDAAESRTFRTA
                     SVAQQLQLITQTLNEEREALSRIRDQVTTLRSTVDGAMDSVGFDDSTGAAVGRSIQDI
                     SRLARQQTAEAESLRNVVEQLRKDSEKLSLLIQAIQTRATEELASTSGNWGIGAANRG
                     RRTGDSLTRSSTNLAQVGSRPLAAASSQALRPIGSSSGPSPKTSGATGTASGERRVSN
                     PGSAHSPRSSTSGSSPGLKPVGSTSSPGVRPVSASSQSLRPISAPDGVVSKSSSGIDR
                     EISGKRDRDRVSSSNADLRLSAQKIGSRPPSDPKPGDG"
     gene            complement(15390..16130)
                     /locus_tag="PKL17_11310"
     CDS             complement(15390..16130)
                     /locus_tag="PKL17_11310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNK45366.1"
                     /translation="MGRTFAIGDIHGEATHLERLLAHLPPLTSDDTLVFLGDYMDRGP
                     DSARVFDIIWSLPQKVPAKVVPLLGNHEEAWLRCLNHPEPGFLLRPDNGCFQALQSIA
                     HVDALPEIEQAAMLLSPQKWFPPELATWLTSLPLYYEDEHAIYVHAGLDGEGEVWHHP
                     RDSRPRNLLWCREPDFFRGYKGKRLCFGHTITKELPVDHLSWFAKILDDPTDVWFRGD
                     LIGLDTACGKGGYLSAVELPSCKIYTSR"
     gene            complement(16188..16604)
                     /locus_tag="PKL17_11315"
     CDS             complement(16188..16604)
                     /locus_tag="PKL17_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNK45367.1"
                     /translation="MTFAKLALGLCGLLVACNRPAKDPANPPAAEAPQIESNLTVSQA
                     AELIKTGPQVQLIDVRSNAEWAGGYIPGAKHIPVDDIPTRMAEIDKSKTVLLYCAAGG
                     RSHRAMEMLKAAGYRDVRHLADGISGWKAAGLTITK"
     gene            complement(16680..18245)
                     /locus_tag="PKL17_11320"
     CDS             complement(16680..18245)
                     /locus_tag="PKL17_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45368.1"
                     /translation="MSQQSLVPWLGEHLLHLCSPAQLGIVCAELAIELWKVAPAQLQP
                     AERVDLLIEHLIATKRLEPFAAALLRDENHRDEALRLGLRTLIPAEQVNPATVPAPHG
                     SDPRTTKSLPIDEAALQARLDAFDERLAAEDPLGAYTLLADGLGGFAHLGCRLGRSRL
                     LLELIRRLYPLAAPLSVEDVAWRNRYAQLLWWETEALRLTGQLDAALVTTERQWPFHA
                     EMLPGLWLKQAQIHRLAGRLSLASSLAKKAQALSPDLSLQAAAAAEQASLALLAGDTS
                     LCLLHLWQARSLLRDAGQNVAPWPLRLALIFAQRALRMQDVTAAREWLTDCVDRATET
                     GDTLALAEASVLLADALRRDGEHEAAAQKLSQATQFASHSGAAEVLVAAGREEARLLM
                     DQASYEAAAAALGTALAMAEELSLACYRIDLLNLRGQLQLRRSNPMSAERDARDALAH
                     AMAPECGYQWGEADSLHLLAVTLLSSRPQVQSLKHSEAIDHLSDELELRDRMQDPTAP
                     EVRWLIRRLRTVA"
     gene            complement(18245..>18445)
                     /locus_tag="PKL17_11325"
     CDS             complement(18245..>18445)
                     /locus_tag="PKL17_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK45369.1"
                     /translation="MPRIFEPFFTTKGDRGTGLGLGICRSIVSQHGGQLLLESPLPHS
                     ASRPPGSTSAGTRVTIVLPRVA"
BASE COUNT         3392 a         5519 c         5850 g         3657 t
ORIGIN      
        1 ctggaagaag tctggcccac agtcgatgca tggctttgcc atcgagatcg cggtggcaaa
       61 cgaccggcgc agatcgggcg actccttacg cagactccga tgcaggattc ttcgtcgaga
      121 gcgcgtgatc tgcggctgct ttctgcgggt ccgcgcgcac tgtggctgaa gaccggacgg
      181 atggcgctcg acgtaagcaa aggaacacgc tggctgcgct ggcagcttcc gcgcgtgatg
      241 cagcttgtgc ggttttggga cagcgccacg atgtcgattg gcgcgacgct ggcgcagcaa
      301 gcgcagacga ttccggatcg gacgtttttg ctgtgggaag gccgcgcgta ttcctatgcc
      361 gatgccgacc ggcgggtcag tgcgatggct ggtgcgctgt atgcgtgcgg aattcgcagc
      421 ggacatacgg tcggactgct gatggacaat catccggact gtctgaccgc gctggtcgct
      481 tgcagtcgga ttggcgcgat tccggcgctg ctcaatccgg cgacacgcgg ggcggcgctg
      541 gcccatgcgc tgcaagtcac ccaggtgcgc gatctgatct cgctcgcgtc ggcgccggac
      601 ttgacccaga cgtatggtgt gcggcgactg attttgggcg gcggtcctcg cgatccgctg
      661 caaagttcgc agcaggttgt cgaccttgaa acgatcgctg ccgatctgcc cagcacactt
      721 gtgcaaaacc ccggtcatgc gtccgacatc gcatggctca tgtttacgtc gggaaccacc
      781 ggcaagccca aagcggtcaa gatatcgaat cggcgctggc tgatggcagc gacggcagcg
      841 gcaacaggat gtgatttgac ccaggccgat acggtgtact gctgcttgcc gcttcaccac
      901 ggcaccgggc ttctgctcgc ggcaggaggc gcgctgctag ggggcgcacg gctcgcgatt
      961 gcgccacgtt tttcggtgcg cggtttttgg gaagatgtcc gccgaaccgg cgccacggtg
     1021 gtgttttacg tcggcgagct gtgtcgttat ctggtgtcgg caccgccgtc accgctcgag
     1081 cgcagccacg cggttcgttt gttcgtcggc aacggcatgc gcccggacgt gtggcgggaa
     1141 cttctgcgtc gcttcggtca ggtcagcgtg ctcgagtttt atgcggccac cgaaggcaac
     1201 gcggtgatga caaaccttac cggcgaaaag atcggttcgg tcgggcgcgt gccgctcggt
     1261 ttggtgcgga cgctggtggt caaatacgat cccgacagcg aaacctatcc gcgcacagcc
     1321 gacggatttt tgattcccgc ggcggtcgac gagccgggca tgcttctgat cgaaattcag
     1381 aaccggaatc cgctgtcgcg cttcgatggc tacaccgatt cgcgggcgac cgaggccaag
     1441 attttgcgca acgtgctgcg ccaaggagac gcctggttct gtagcggcga cttgatgcga
     1501 gaagatgccg atggcgactt ttggtttgtc gatcgcgtcg gtgacacgtt tcgttggaag
     1561 ggcgaaaatg tgtcgaccga gcaagtcgcg gctgtccttt ccgagctttc gtttgtcgaa
     1621 atgtgcgcgg tgtacggcgt gcggcttccc ggtcaggaag gacgggccgg aatggccgcg
     1681 atcaagctgc gtcccgagca acatctagat ccggaagcgc tgtttgctgc ggtcgatcag
     1741 cacctgatcg atgctgcgcg tccgcggttt gtccgcatcg tgtcggagct gcaaaccacc
     1801 gagacgctga agatcgtaaa gcagccgctg cttgcggatg gattcgacgt gtcgcggacc
     1861 ggacccctgt atgtgtacga caagccgcgt cgtacctatg taaaagccgc agccgacgag
     1921 ctttccgcgc tgctaccgac gttgtaagtg ccccgtcagc ctggccggtt tcggaccgct
     1981 acaatccgag tgacgcggcc tggatctctg ggcctgctgt accaggcggg tacatcgccg
     2041 cggttttctt tcactcgcgg gaaagtggtc aggctataat gacttaaccc ttaaggtcat
     2101 cttgggctcg acgacaaata ccgctggcgc tgctgctgtg tctgcccctc ggactgctgc
     2161 tgtgaaggcc gcctattcga cgatggcaag gctctgccag tcggtcggtc aggtggtaca
     2221 tggcaagtcc gaggtgattc gcctcgcggt ggtttcgctg gcggcgcgtg gtcacatcct
     2281 gctcgaggac attcccggcg tcggcaagac caccctggcg cgggcgctgg cgcgggcgac
     2341 cggcgggaca tttcggcgca tccagtttac gtccgaccta ttgccgtctg acgtgattgg
     2401 cgtgtcggtc tggaatgagg agtcgcggca gttcgatttc aagcacggac cgctgtttgc
     2461 caacgtggtg ctcgccgacg agataaaccg tgcggcaccg cggacgcaga gcagcttgct
     2521 cgaagcgatg agcgaaggcc gcgtgtccgt cgaccactac acctacacaa tcgagcagcc
     2581 gtttctggtc atggcgacgc agaacccgct cgaacacttt ggcacctatc ctttgcccga
     2641 atcgcagatg gaccgctttc ttttgcgtct gcggctcggc tatccgtcgc gatacgacga
     2701 acggcgggnn nnnnnnnnnn nnnnnnnnnn nnnnngtgga agacatgaag ccggctgtgg
     2761 cgcccgctga agtggtgcag ctacaagaca ccgtttgcat ggtgcgcgtc gaggaatcgc
     2821 tgctcgaata tgccgagcgc gtcgtcgctg agacccgtcg ctcgccgctg cttcagcttg
     2881 gggtgtcgcc gcgtggtttt cagtcgtggt ttcgcgctgc gcaagcgcgg gcactgtcgg
     2941 aaaatcgtga atactgcgtc cctgatgact ttcgcgagct ggccctgccg gcgctggccc
     3001 accgtctggt gctgccggga agcagccaag gtggctatag cgacgcgctc agcaatggtc
     3061 gcgccgaggc agagcgcatc ttgaccggga tcatcgagcg cgttccggtg ccatgagcct
     3121 gcgctttatc acccgtctgc gcggcgtgcg tttgacccgc gatgggtggt ggttctgcac
     3181 cgctggtctg tgtctgtttt tcgtcgcgct caacagcggc aataacctgt tgtatttgct
     3241 tctgtcgatg ctgattgcgc tgatggcggt gtcgcttgtg cttgccgaag cgtctctgcg
     3301 cggggtgatt ccgcgccggc aaccaccgcc gcgactgcat gccggatcgc cgtttttgat
     3361 gggcatcgcg ctgcgcaacg acaaaaagca gctgccgtcg ttttcgatcg aagtcgaaga
     3421 cctgtgcgac gacaaagtgg tcgacaagcg ctgttatttt ttgaaaattc cgtcgggacg
     3481 aacgcagcag acggcgtatc ggcacacgct gccccggcgc ggacggtatg tcttttcagc
     3541 gttccggatt tcgaccaagt ttccgttcgc gctgtttcgc tcgtcgcgct acatcgaaca
     3601 tcggtccgaa cttctggtct atccggcgct gattccgctt gagcactggc ccaaggcgct
     3661 gacgtcggca gtcggtgaac agtcggtgca gcggctgggg cgacgtggca actttcatgc
     3721 gctgcgcgag ttccgtcagg gcgacgaccc gcgtgacatt cactggcgca gctcggcgcg
     3781 cagacagcgg ctgatggtgc gtgaacacga aatcgaagct gtgcagcagg tgacgctgta
     3841 catcgacaac tcgctgccga cggataagct gaatcttgct gacgagcagg ccaagctgga
     3901 aaaagtgatc tcgtggggcg cgtcgctcgg ggcgttttac ctggaccacg gctatgccgt
     3961 gcggctgtgt gtgcgcagtg aggagccacc gctgccgctg ttgtggagcc cagccagctt
     4021 gcaaaaactg ctgaccacgc tggcgctgct gccgacggta tcgcccgatg ttccgtttca
     4081 aaaaccaccg cctctgcccc gtcatgctgc cgtcccagtc gcagagcacg gcgacagcct
     4141 ggtggtgatt cgtcccggcg caccgattcc gcgcgaaaaa cacatgggcc acgtcgtgga
     4201 agcatcatga ggtttgccgc aacgcacaaa gtggtgtcgt acctgatggt gacgacggca
     4261 tttttgatgc tggcgctgac cggagagctg ccgccgtggc ttctgctgct gacgctggct
     4321 gcgatgggtg gatcgtactt ttttgatcca cagcagcatc ccctgatgtt gcgccgctcg
     4381 tacaactatt ccctgtacgg tgcgatgctg cttttgtcgg cgcttttgct cggcaatgcc
     4441 agcgggcccg aagcgctgtg ggatggtggc ctgcgcatgc tgtgcgtcct gctcgtcggg
     4501 aaattgtggc aaaggcggca caacggcgat tacttgcagg tctatgtcgt cagttttgtg
     4561 atgctgctcg gggcgacgtt tgtggaaagc agcatgctgt atgcgctgct tctgtttctg
     4621 tatgtcgtct ttgcgatgtg gacgctgacg ctgtttcacc tccgtcgtga aatggaggaa
     4681 aactacctgc tcaaacacct gccggggcgt cacggccagg ccgccgagag tgagcgcgtc
     4741 gaagtcgagc gaatcctcaa ttctcgtcgg gtcgtcggtc cgccgtttct tttgaccacg
     4801 gcgctcgcgg gtctttgcac gttctttgtc gcgctcgggc tgtttctgct gctgccccgc
     4861 tttcatgtca ccgtcgagct gccgtttcat cggcgcgcgc tgccatcgac cggattttcc
     4921 gaacggctcg agctgggcag tcatggtctt ttgcgcgaca acccgcgtgt ggtgatgcgc
     4981 gtcgaactgc ccggacaaaa tccaccgtcg gtgctgcggc tgcgtggcgt gtcgtttgcg
     5041 cgctacgagc agggtcgctg gtcgcagcat cgcctgggtg aacagccgct tggggtcagt
     5101 caaggcctgg cgcagattga tgccgtccgc gatttacacc agccgccgct gcgccggacc
     5161 gaaatttacc tggagccgct cgacgccgaa gcactgttta caccgacgcc gggctatccc
     5221 gtcgcgctgt atttgcccga tgcgtttgcg gcgatttctg gcttgctgac cgtgacgtcg
     5281 gatgggcagc ttgcggcacg gggccgacgg gcgccactgc attacgcggt gctgtcttcg
     5341 tccgaatcgc ccagtttgcc gacagacgat ctggccatct atcgtgaact ttcgtctgat
     5401 ctgcggctgc ggctgcgtcc gctcgcgctt cagatggtcg gggatgcaac ccaaccgagc
     5461 gttcaggcgg agcggatttt ggcctacctg caaaaaaact acagctacac cacgcggctg
     5521 tggaaaccgt cggaacgcga gccgattttt gaatttttgc agcgcgagcg ccgcggtcac
     5581 tgcgaatact ttgccaccgc gatggcggtg cttttgcgca gcctcgacat ccccagtcgc
     5641 acggtgaacg gctatctgtc gccggaatgg aatcgctacg ggcgcttttt tgtcgttcgt
     5701 cagcagcacg cccactcgtg ggtcgaagcg cttttgccag gccaaggctg ggtggtgctg
     5761 gaccccacgc cgtcgcagag cccgcctgcg ccatggccga ttctgcatcc tctccgtcag
     5821 ctattcgacg gtctggacat gagctttggc aaatacgtgc tcgaatacga cgccagtgcg
     5881 cagcagcacc tgggcgagcg actgttgcga ctgtggcaaa agcgggctgc cagcaagcag
     5941 atcgacccag agccgccgtc ctttacgtac aaaaagctgc tgcgcagtgc gcttttgctg
     6001 ctaagcagcg gcctgtttgc gtggcttttg tatcggcggc tgaaacgacc aagcgacgag
     6061 ctgctggcgg gtgaaagaga agggcgcgga ctgcttgtcc aagccctgac ggtgctgcga
     6121 cggcgtggtt ttgtgcgcgg accgggggaa acgctgcgca agctatccga gcgtatcagc
     6181 gacgacggcg atccaatcgc gccgcatttt tcgcagctcg ttgagcatta ctacgcgcat
     6241 cgttttgggg accagccgct ggatcgggcg cttgtggacc ggcttatcga tcagatgcgg
     6301 gcgacgccgc ggcgcccccg ccccgagatg agaaactagc gggtcagctt tttgtatttg
     6361 atgcggtggg gctgatcggc gtctttgccg agccggcggc gacgatcttg ttcgtagtcg
     6421 cggcagttgc cctcaaacca caccactttg ctgtcgcctt cgaaggcgag catgtgcgtc
     6481 gcgatgcggt cgaggaacca gcgatcgtgg ctgatgacca ccgcgcagcc ggcaaagctt
     6541 agcagcgctt cttcgagcgc ccgcagcgta tcgacgtcca gatcgttggt cggttcgtcg
     6601 agcagaagca ggttgccgcc cgcgcgcagc atcttcgcca ggtgcacacg gttgcgctca
     6661 ccgcccgaca gatccttgac gcgcttttgc tgatcctgac ctttgaagcc gaactgtccg
     6721 acgtaggcgc gcgactggag cttgcgcttg cccatttcga agttttcgac gccgcccgag
     6781 atttcttccc acaccgtctt gtcgcccttg agcgcgtcgc ggctctggtc aacgtaggcc
     6841 agcttgacgg tttctccgac gacgagcttg cctttgtcgg gtttttcctg gccgacaatc
     6901 atgcgaaaca gtgtggtctt gcctgcgccg tttggaccga tgacaccgac gatgccaccg
     6961 cgcggcaaac ggaacgacag gtcgtcaaac agcagccgat cgccgtaggc tttttgcacg
     7021 ccttccgcga cgaccacgat gtcgccaagc cgcggtgccg gcggaatcga aatttccgcg
     7081 acttcgatgc gtgcttcctg cgactgcttg agcagatctt cgtaggcggc cagacgcgct
     7141 ttgcttttgg cctggcgggc tttgggtgac tgccgcaccc actccagctc gcgcgccagc
     7201 gtcttttgcc gcgccgattc ttgcttttct tcgacggaca accgcttctg tttttgatca
     7261 agccacgatg agtagttgcc ttgccacggg atgccttggc cgcgatcgag ttccagaatc
     7321 cagcccgcga cgttgtcgag gaagtagcga tcgtgggtga tggcgacgac ggtgccgggg
     7381 aattcggcaa ggaaccgttc cagccaaaac accgagtcag aatcgaggtg gttggtcggt
     7441 tcgtcgagga gcagcatgtc gggccgttcc aaaagcaccc ggcacagcgc gacgcgacga
     7501 cgttcaccac ccgaaagcac cgtcacatct tggtccggcg gcggcaggcg cagcgcatcc
     7561 atcgcgattt cgagctgccg ttcggtatcc cacgcgcctt tggcttcgat ttcgtcctgg
     7621 acgcggctgt gctcctcgat gagcttgtcc atttcggcgg cgggcaggtc ttcaccgagc
     7681 cgcatcgaca gttcttcgaa ccgcttgagc agatcgagcc ggtcttttac cgcgatggcg
     7741 acgttttcaa agacgttctt gcttgggtca agctgcggtt cttgcggcag ataaccgacg
     7801 cggattccgt cggcgggacg ggcctcgcct tggaattcgt ggtcgacgcc cgccatgatg
     7861 cgcaaaagcg tacttttgcc cgcgccgttc gagccgatga cgccgatctt ggcgccaggc
     7921 aaaaacgaca gccagatccc tttgaggatc tcgcgctgcg gcgggaccac tttgcgcagg
     7981 ttctgcatcg tgaagatgta ctgagcggcc atttctccct cttggaaaaa tcaagtgagc
     8041 ttttaggttc gcgcgagcat cccgctcaag ctgtacgaga ggatctcgcg acagacgcgg
     8101 tgcaggtttt tttcgtcgga gtccctgcgg acgatccatt gcaaagcgac ttctttgagt
     8161 ccaccgagcg ccgcctgcga cacaatttcc ggatcgatcg gtccaaacag acccatcgcg
     8221 cagcctttga cgagcgcacc cttgagcaga agccgcactc gttcgtaaaa gtccgccatt
     8281 ttgtcgtcgg cgtcgctgtc cagaccttcc gccatcagga gcacgattcg ggtcatgtcg
     8341 cgattttccg acagcacgga catcacccga cgcaagttgg cttcaatctg cacaagcggt
     8401 ggcggcgaat cgggacttac atcaaccagc gtgacgacgc tttggatgct ggtcagaaat
     8461 gcgtcgacca gtgcatcgaa catcgcgcgc ttgccgtcga agtgcagata cagtgtgccg
     8521 cgggcgactc cggcggtatc caagatgtct tggatcgatg tcttgtgata accccgttcg
     8581 gcaaacaggc gtcgtgcgac ggacagaagc tgcgtcgagc gctcgtggcg gagccgctgg
     8641 gcacgggcca cccgtccatc catcggctcc gtcgtcgctg tgccaatcga acccaatcgt
     8701 tccccgacat ccggggtcga ttcggcaacc gctagcgctg cggtcgatcc tgactgggtt
     8761 tctggctcgc tctttaccac ggcgcgcatg ctaccagcac tttgccgctt gtcgctgctt
     8821 ctgtcgcaga tattcgtgcc gggtcgcatg ctgctgcgag tgccccacaa agtaatcgaa
     8881 taacctagcg atactgcttg acaatctggg tgcgcatgga agaagataat tcttgtgaag
     8941 tggctaacta gttccgaggt ggctaaccaa ccgctcccgt agcggaagga aggcagcaaa
     9001 gaaccggaaa gcccgaagaa attttcggca aagagcaacc aaagctcaca atcaactgtt
     9061 agggaaaacc cccctaaaat ggaggccact atggctggtt cggacaagag aaagcagagc
     9121 ctgtacttcc ccgaggatat gctaaaggag atccaggagg aggcgacccg tcaggatcgc
     9181 tcgctgtcgt ggatcgtcca gaaggcatgg aaaattgccc gcaacgagat tcgcaggtat
     9241 ccctcggtca atgaaatcga gggaggagaa gacgacgagc ggggcagcaa ggaatagcga
     9301 cgaccccaag cgggacacct cgtcccgcgg tcgtggcaag cggcgcagcg gctttgcaat
     9361 aagccgtgat ggcgccgcta ttttttttga agtcctggga cagccgcatc cggcgccgac
     9421 tttggtgctc tgcgacgggc tcggctgtga tggctacgtc tggaagtatt tgcagtgggc
     9481 gctcgccgac aagtggcgca tcgtccattt gcactaccgc ggacacggac agacgccgct
     9541 gcccaaagac tttggccagg tttcgatcgc ggactttgcc accgacgtcc tggcggtgat
     9601 ggacgcgagc gagacagagc gagcggtcat ctgtggtcac tcgctcggcg tgcaagtgtg
     9661 cctggaagtg tatcgccagg ggcgcgagcg cacagacgga cttgttctga tctgcggcag
     9721 ctatcgcaac ccgcttagca cgttcaaagg caaagaaacg ctcggcgacg tgctgcccat
     9781 cctccaagcc gcggtgagcc gagtgccacg cctcgtcggc gcgttttgga aacggctctt
     9841 gccgctcgat ctgtcgtatc agattgcccg gcgcttcgag ctaaacggtg agctggtcca
     9901 tcgcgaggac tttgcgccgt atctcgacgc gctgtcgcga atggaccccc ggatgtttgt
     9961 cgcgacgatg gccgccgcgg gtcagcacga tgcgcaagat gtcctgccgc agatttccgt
    10021 gccgacgctg cttgtcacgg cgcgacatga cacgtttacg ccgatgaagc tgtcggaaga
    10081 aatgcgcgaa aaaattcccg gttccgagct gcatgtggtc gaagcgggca cgcacaccgc
    10141 gccgatcgaa agaccgtcgg aagtgtcgca agtggtgcgc agttttctcg agcgacggat
    10201 acttggacag tgaacgatcc tcagtcgcaa tcggagacgg ctccagatgg agcgctcaca
    10261 ccgcgcattc actgggtaaa ccacgtactt ttgtggttta tgcgcgtcgg catcgtcacg
    10321 atgatcatcg tgaccggcgt gtatatgtac ctgcgcgaca agccatcgcc ggaacaagag
    10381 gagatgattc gctacgtcga ggtgaacatc cccgcgctcg aataccacga ggcgccgatt
    10441 ctttcgcagc tggatgcgct tttgcgggac aagtcgctgc gtccagaatc ggcgcgcaag
    10501 cagcttgccg atgagctgat gccggcgctg gttcggctgc gcaaagtggc cgaaggtccg
    10561 gtttcggtgg ccaagacgcc gccggtcaag cagctcgccg aggagtaccg gcagagcgtc
    10621 gaagagctta tcaatgtctg tcggacgacg atccgcgtga tcgatgatcc gaagcttggc
    10681 gagcgtgagg gctatcagca agtgctcgac tcgctgcgac gcgcgattgc ccaaaaccgg
    10741 acctggcgcg atcacgtcgc tgaaaccacc catcggctcc agcttggcca gcgcgggctg
    10801 cacaaaaagt aacgccgctt aggtcgccgg actgggcgcc gtgatcgaca gcgcgtgggc
    10861 gagccgaaac atggcgcaga gcaagtgacc gtcttcgatg tgttggcgct gcgcgaccag
    10921 ttcatccagc ggtacaaacg ccagtgccga cgaagcgagc gtttcggctg cgacttcggc
    10981 gaccagcgga tagaccactt cgggcgttgc gccgatcgac ggatagtatt tgccgccgag
    11041 cggaaacacg cgccgcgctt tgacgccaaa atgggcgagt aggttgcgca aaaccagggc
    11101 ctctgcgtcg ggaaatgtct ggatggtctt gggcagtcgc cacgccggac acgtcagcag
    11161 cgtcgaatgt tgtcccgccg tgtggagctg aaccgccggc agatcgcgcc gctctagccc
    11221 gaccaaatac tggttgccca gacgaaccac cgggatgacc gacacggtgc tcagtccaaa
    11281 tgagcgcggc gcgcacagct ccagctcggt acgacgaagc accttgtcgc tcgcgtccag
    11341 ctcttcgacg gtcgctttat aaagcgaaag aaaaccgtgg gctgaatcac tggaatcgac
    11401 ctgggcaaat gccggctcgc ggcgggttgc cagcacgtcg gcggtgatcg ccgggacaaa
    11461 accgggcgcg gtctgcggcg acagcgaaag ttcaattcca atccaaggac cgagcgcttc
    11521 cccgaggctg agcagcaagt gatagacgct gatttcgagc cgagcatctt gcagtccacc
    11581 gacttgggct gcccgcaaaa gctgcgccgc gacaaacggc tgcacccgtc ctgcggtcgt
    11641 aaacggtgtg tagttttgta cttcgtcgct gaacttcgga aaaccgtcgc tgtgctgtgg
    11701 cagtttgctg acatccagct cgcacagccg cgtgctgacg tgttcgctga gcccgcccgg
    11761 cgacgtgtag tagccaagac cgctttggat cgccgcgatc gcttcgtttg gcagcccagc
    11821 gcgggtggcc aaaatcgtcg ctgatgcctt atcggaatgc gcaaaaggca agtcgcggac
    11881 ctgcgccgtg atggtttcgg tcaaataacc agcaacgtgg gctccgtcga gcgccggtcc
    11941 gtcgagcaag gtcgtcgaaa gtgggcgcgg gaaaccatgt ttggcgagca ccagcacccg
    12001 accgccatgt cgaaaccagg gaatgacatc gagcacggga ttgggtcgct cggcgagctg
    12061 aaacagctgc ccggtttttt ggtgccgcag cgtgtgcagt cgcaaaaagt ggggcgtcgc
    12121 aagcggcgca tggtcgacgg caagtcgcac gccatgatgc ggcgcgactt tttccccaac
    12181 gatcaggtag ttcgtcggtg gaaacggcag cggctcaccc gagatttggt gtagcgcaca
    12241 gcgaccgcgg aaacgatttt cgatgatcca gggattgtgc agctcgacgg acagcacgat
    12301 gcgcaggccg tggcgacgga acgccgcttc gtactcggct tggctgtaaa acgtatattc
    12361 ctcgcggcac tcggcatccc agtcgctgcg atagtctttg tggagcagaa actccgcggc
    12421 agcgcgaccg gcgaccaaaa aacgcgacca gccgggctgt ggcgacggaa gctgctgata
    12481 cggaaccggc ccggctgggt tttgactgga tcgaaactgc gcacaaaagc ggtgaaaaag
    12541 cgccgccgtc gaaagccccg tcacagctcc gtcgcttgtt ccatcgtcgc ttggcagatc
    12601 gatccagacc tcagtgggaa aacgtggcac cacaaagtca cggacgacga gcacgccacc
    12661 tgggcgcagc gcttccgcat ggtgacgaag cgcccgctcg acttcgcgat gggaaaaacc
    12721 gttgaagctg gtcaggtggt gccacaccga cgaattgagg atgccgtcga cgcttcccgg
    12781 cgcaaacaac ggatcggcaa tgtcaccctg ctgataacag aggtttgcgc gctggtagtg
    12841 ggcacgggcg tactcgacgg cttgcgcagc gatatccacc ccgatgacgc gcagcccgga
    12901 aaacaggctg gccaggtcaa acgaccctga acccgatcca cagcccatat cgaccaagat
    12961 gccgctgggc gggaagaacg ccgtcgtcag cgcgaccttc tgctgcatcg acttgtccat
    13021 gccggcgtaa tacgaggcat atgcatcgtc gtcgccgcgc gcctgcgccg cgtagctgtc
    13081 gctcccactt gcaggaattg aagcgctgtt tgctgcgtct gtcatcgtcc gtcgcctagt
    13141 tggattgtga ttttagtaat tcaaattact aacgtctgta ttgaagcggg aaacttttgt
    13201 acgcttatca aaatggccga aaccacaagc tcggaacgac aggcgccgtc ggctacggct
    13261 tcaacgtcgg gcggccagcg agcagagatc ccgcagcagc ggcggctggc tgtcgttact
    13321 tggctgatgg ccgcgctggc tagtttgtta gttggattac cagctctgtg gcttcatcag
    13381 cagcagtcca ggcacgccgc actggtgatc gtcgaaaatc aagccaagct ggcgagcctg
    13441 gcgatgacgc atcacttgac gctgcgccga gagagtcgcg cgtcgatgtt ttcgtcggtg
    13501 gcagcgcgcg tcaggccgat cgaagccggg caccgtgtct gggaacatct ggcgcaagaa
    13561 gcgcgtgcgg tcggggctga tctggtggcc gtcgtcgatg aaggcggggc acttttggca
    13621 caggatgggg caggtgcgat tgcactggcg gaagcggcgg cgcgcagcgg cattgcccca
    13681 agcggtgcgc tcttggtgat gggcgcgcag atcgtggaag cgttccgcat cccggtggcc
    13741 tacgacggca agcaggcgtt tttgattgcg gcgtgccgaa tcctgcccga atcgctgcgc
    13801 caagacgccg agccgctcgg ccttggggtg gcgatcgcgc tggaaggatc gtttgtaacc
    13861 aatctgccgg cggatgcgct gagcagtggc cgctggggcg atgacccgcg tgagtcgctg
    13921 cacaacctgt cggtgcggta tcacttgcgc gcaacaccgc tgtggcaagg tcggctgctc
    13981 gtcgcggttc cgctgcaaag cgtccagcag tggtcacgcg atcagctcat cggttcgttg
    14041 ctgcttgtcg gacttttgct gggcggtgtg ctgctgttgg cgctgtgggt gcagcttggc
    14101 gtgatgtcgc cgctgcttcg catgtcgggc gccgcgagcc gggtcagcca aggatcgctg
    14161 gcgcgcggtc gggcgcagct taagccgttt ttggatcgca acgacgagct ggcgacgatt
    14221 ggcaaaggac tcgattgggc ggtgcagcga ctgcaaagcc tgctgcacac cagcaaccgc
    14281 gtgctcgacg acattgacag caccatcgcg acgattgaac ggagcgccag cacgctggcg
    14341 acttcatcgg gaacccagga agagcgcagc aaagaagtcc aggcgacggt ggtgccgatt
    14401 acgcgggcga ttgaacggct gacccggcaa atggtcgagg ttcgcaacgc cgtggtgcaa
    14461 atttcgctcg cgtggtcaaa cgccgatcag gcccaagcgc aggttctgtc ggcagcgcga
    14521 cgagccgatt cgatgctaca caaccccgat gcagcggagt cgcgaacgtt ccggactgcg
    14581 tcggtggccc agcagcttca gctcatcacc cagacgctca acgaagaacg cgaagcgctg
    14641 agccgcatcc gcgatcaagt gacgacgctg cgatcgactg tcgatggcgc gatggactcg
    14701 gtgggtttcg acgactcgac gggcgcggcg gtgggtcggt cgatccagga catttcgcgt
    14761 ttggcgcggc agcagacggc agaagcggaa tcgctgcgga atgtcgtcga acagcttcgc
    14821 aaagacagtg aaaagctgtc gctgctgatc caagcgattc agacccgcgc gaccgaagag
    14881 ctggcttcga cgagtggcaa ctggggcatc ggggcagcca accgagggcg gcgcaccggg
    14941 gattcactca cgcgatcgtc gaccaatctg gcgcaggtcg gttcgcgacc gctggcggcg
    15001 gcatccagtc aggcgctgcg tccgatcggc agctcgtctg gaccgtcacc aaaaacgtca
    15061 ggggccactg ggaccgcgag cggtgagcgg cgcgtttcga accctggttc ggcgcacagt
    15121 ccgcggtctt cgacatcggg atcgtcgcca gggctaaagc ccgtcggttc gacttcttcg
    15181 ccgggtgtca ggccggtgtc ggccagctct cagtcgctgc gtccgatttc ggcacccgat
    15241 ggtgtcgtca gcaagtcgtc gagcggcatc gaccgtgaaa tatccggcaa gcgtgaccgc
    15301 gaccgcgtca gctcatcgaa tgccgatctg cggctgagcg cccaaaaaat cggcagccgc
    15361 ccaccgagcg atccaaaacc cggcgacggc taacggcttg tgtagatttt gcatgacggg
    15421 agctcgacgg cggacagata gccgcctttg ccacacgcgg tgtcgagtcc aatcaggtcg
    15481 ccgcgaaacc aaacatcggt cggatcgtcg aggattttgg caaaccagct caagtgatcg
    15541 accggcagtt ctttggtgat ggtgtggcca aagcacagcc gcttgccttt gtagccacgg
    15601 aaaaagtcgg gctcgcgaca ccaaagcagg ttgcgtggtc ggctgtcgcg cgggtgatgc
    15661 cagacttccc cttcaccgtc gagcccggcg tggacataga tggcgtgctc gtcttcatag
    15721 tacagcggca aagacgtaag ccacgtcgcc agctcgggcg ggaaccactt ttgcggtgac
    15781 aagagcatcg ccgcctgttc gatttccggc agcgcgtcga cgtgcgcaat tgactgcagc
    15841 gcctggaagc agccgttgtc gggacgcagc aaaaagcccg gctctgggtg attcaggcag
    15901 cgcagccacg cttcttcatg gttgccgagc agcggcacca ctttggccgg caccttttgc
    15961 ggcagcgacc agatgatgtc gaacacccgc gccgaatcgg gcccgcggtc catgtaatcg
    16021 cccagaaaca ccagcgtatc gtcgctggtc agcggtggca gatgcgccag cagccgttct
    16081 aggtgcgtgg cttcgccgtg aatgtcccca atcgcaaacg ttcgccccat ccggcccatc
    16141 gtaccgcgaa gggctggctt tttggcaact cacagcgacg ggccaagcta cttggtaatg
    16201 gtcagaccgg cagctttcca acccgagatt ccgtccgcca ggtggcgtac atcgcgatag
    16261 cccgcggctt tgagcatttc catcgcgcgg tggctgcgac cacccgcggc acaatagagc
    16321 agaaccgtct tgcttttgtc gatttcggcc attcgggtcg ggatgtcatc gaccggaatg
    16381 tgcttggcgc cgggaatgta gccgcccgcc cactctgcgt tggatcgcac gtcgataagc
    16441 tgaacctgcg ggccggtctt gataagttct gccgcttggc tgaccgtcag attggattcg
    16501 atctgcggcg cttctgccgc cggtgggttc gctgggtctt tggcgggccg attgcacgcc
    16561 acaagcaggc cgcacaggcc cagtgcaagt tttgcaaacg tcatgagcgg tcttctttcc
    16621 gaaacaggaa aacggggaaa acggcagaaa acgggccaaa tctggccaaa acggcgactt
    16681 taggccacgg tgcgcaggcg ccgaatcagc cacctgactt cgggcgcggt ggggtcttgc
    16741 atgcggtcgc gcagctctag ttcgtcggac aagtggtcga tggcttcgct gtgcttgagg
    16801 ctctgcacct gcggtcggct cgatagcagc gtcacggcca acagatgcag gctgtcggct
    16861 tcaccccact gatagccaca ctcgggcgcc atcgcatggg ccagcgcatc gcgcgcatcc
    16921 cgttctgccg acatcggatt cgatcgtcgc agctgcagct ggcctcgcag gttcagcaga
    16981 tcgatgcggt agcacgcaag tgacagctcc tcggccattg cgagcgctgt gcccagtgcc
    17041 gcggcggcgg cttcgtagct ggcttgatcc atcagcagcc gtgcttcttc gcgacccgcc
    17101 gcgacgagca cttcggcggc gccgctgtgg cttgcaaact gggtcgcttg cgaaagtttt
    17161 tgggccgccg cttcatgttc tccgtcgcga cgcagcgcat cggcaagcag caccgacgct
    17221 tccgcgagtg cgagcgtgtc accggtttcc gtcgcgcgat ccacacagtc ggtcagccac
    17281 tcgcgcgctg ccgtcacgtc ctgcatccgc agcgcccgct gcgcaaagat gagcgccagc
    17341 cgcagcggcc aaggcgccac gttttgtccg gcatcccgca gaagcgaacg cgcttgccac
    17401 aggtgcaaaa gacacagcga cgtgtcgccc gccagaagcg ccaagctggc ctgttccgcc
    17461 gccgctgccg cctgcagcga cagatcgggc gacagcgcct gggctttttt ggccagcgat
    17521 gaagcgagcg aaagccgccc cgccagacga tgaatctgcg cttgttttag ccacagcccc
    17581 ggcagcatct cggcgtgaaa cggccactgc ctttcggtag tcaccagcgc cgcatcgagc
    17641 tgtccggtca gacgcagcgc ttcggtttcc caccacaaaa gctgcgcata acggttgcgc
    17701 caggccacat cttcgacgga aagcggcgcc gccagcggat acaggcgacg aatcagctcc
    17761 agcaaaagac gcgagcggcc cagtcgacag cccaggtggg caaaaccgcc cagcccgtcg
    17821 gcgagcagcg tatacgctcc gagcggatct tccgccgcca gccgctcatc gaatgcatca
    17881 agccgtgctt gcagtgcggc ttcgtcgatc ggcagcgact tggtggtcct cggatcgctt
    17941 ccgtgcggcg ctgggaccgt cgctggattc acctgttccg ctggaatcag cgtgcgcaga
    18001 ccgagccgca gcgcttcgtc tctgtggttt tcgtcgcgca aaagcgccgc tgcaaacggc
    18061 tccagtcgct tggttgcgat caggtgttcg atgagcagat cgacacgctc ggcgggctga
    18121 agctgcgcgg gcgcgacttt ccacagctca attgccagtt ctgcacagac aatcccaagc
    18181 tgcgcggggc tgcacaggtg caaaaggtgt tcaccgagcc acggtacaag gctttgctgt
    18241 gacatcaggc caccctcggc aaaacgatgg tgacacgggt tcccgccgac gtgctccccg
    18301 gtggccgcga agcactgtgc ggcagcgggg attccagcag aagctgaccg ccgtgctgac
    18361 tgacgatgct gcggcaaatt cccaggccga gcccggtgcc acgatcgcct ttggtggtga
    18421 aaaacggttc aaaaatgcgc ggcat
//
LOCUS       DAORGN010000089        18410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1218_length_18410_cov_6.472242, whole genome shotgun sequence.
ACCESSION   DAORGN010000089 DAORGN010000000
VERSION     DAORGN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_18410_cov_6.472242"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PKL17_11330"
     CDS             complement(<1..1279)
                     /locus_tag="PKL17_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNK45370.1"
                     /translation="MSDLWDIDILNRGKDWVDLKVTLHHPDAGPFPESPGFALQLLLI
                     PAFGWDSSGNRRAPSPLSSEFSEDNYYDAEECADRAKKFVRSVQVYKASNVPFVEKQV
                     HAGVDKRVIERGIARDSDDWESAWENEWRAYWSDPARVPSATYRISVTAPKWIDHIEP
                     GMRFDSASWCPNGPWVNKPRSKTLETPPDAWVKPWLVDFPLSDKPRGTRQMDPQLIES
                     LAASGTPLKGQELATAVAQHKLFLDAGNREGDWQLLSVSGLPLCIYQVYGQSDENKVG
                     AQLVLRMKQIPAGESLRGADIGYADLSGSICRGVDFGKAILDGCAAVDAFFDGATFDG
                     ASLRKVDFSGSSLRGCSFVGADLRGADFEHTDLTGADFGAANLTGARFPGAIFDGVKR
                     DGQVAPTPAKPASARKPAAKKSAAPKKSAAPKKS"
     gene            complement(1316..3742)
                     /locus_tag="PKL17_11335"
     CDS             complement(1316..3742)
                     /locus_tag="PKL17_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNK45371.1"
                     /translation="MDKQGKQDRRFRKAAVLGAGVMGSGIAAHLAGAGLDVLLLDIVP
                     PDLATAQNADKKARNRFAQGGVDKLRTSKPSLIFHQRDLERIRVGNLEDDLAEAAKAD
                     IVIEAVREDLNVKQALFAKLEPLLGAQTVLASNTSGLPIGKLMAGRALDMRRRFLVTH
                     FFNPVRYMRLLELVSGEDTDPQVMCQIAGFGETQLGKGIVYGKDTTNFVANRIGIYGM
                     MGLISEALKAGFTVEEVDAIFGPPLGRPKSAVFRTADMVGLDTVIDVAQNCYDNLIHD
                     EARQVFATPDVIREMVRRGYKGDKTGKGFYQKTKEGLLVLDLNTLEYRAQQKARFESL
                     SAGKSLTDVAERIRSVLSGQDRAAELAWKATALLFSYSSRRIGPRSEVDGNQNDAPIA
                     DDLCQVDRAMRWGYGWEMGPFEMWDAVGVKASVERMEQSGIRPAQWVYGMLSAGRSSF
                     YSGEPGQKHYFDLSAKAEVAVPKSPRELSLAGLSSQGLVIDKNDGARLIDLHDDVYAL
                     EFLTKMNSIDADVIGLLNKAIEICDVRKKALVITNEATDSFSAGANLMLIVMAAMQGN
                     WTMLEKVVADFQNANQLLRYGPVPVVVAPFALTLGGGAEVTMHADAVRAHAELYMGLV
                     EVGVGLLPGGGGCKELICRLQSHLPENADPFPSVQRAFETIGLAKVATSAEEARSLGF
                     LRETDGVTFNRDHLTYDAKQLALGLLRAGYRPPRQRLVRLPGPSGYANIRSSLQMMLE
                     AHQIMPHDLTVGSALAKVLTGGNTSPTVRMTEQQLLDLEREQFLALCGEQKSRDRMMY
                     MLQNNKPLRN"
     gene            3877..4050
                     /locus_tag="PKL17_11340"
     CDS             3877..4050
                     /locus_tag="PKL17_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45372.1"
                     /translation="MGRGDNRNSPKMRRKKAQTRLKNRIKNRRVAAAAVRAEKKAATA
                     AAPAAKPAKKVAH"
     gene            4154..4558
                     /locus_tag="PKL17_11345"
     CDS             4154..4558
                     /locus_tag="PKL17_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45373.1"
                     /translation="MGWRADYVASHGFVETHYEGVLSREDLGAAIERTLALSREHDTT
                     LFLGDCSRLEGGHDIFTLYGFVRLLESLGLVGRFKEALIMPTLAAPARDVEFWETACL
                     NRGLLVRVFPDRESACDWLCQRPYFVQPLNPA"
     gene            4627..5019
                     /locus_tag="PKL17_11350"
     CDS             4627..5019
                     /locus_tag="PKL17_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45374.1"
                     /translation="MSGGWQWLGILLLLQGGCVPQAVYLAKAEEVEQLKSREQLVQQR
                     LGERIDRLSARLSDLEKEIGKTEPRWTELSAAMQLVRDLAVQTDQNLLKLLVQRDTRC
                     DCPSAPPKTPPPFGRGQKTPPLFPSKRH"
     gene            complement(5023..5487)
                     /locus_tag="PKL17_11355"
     CDS             complement(5023..5487)
                     /locus_tag="PKL17_11355"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNK45375.1"
                     /translation="MSETQAEHSHDDFQYPPIIPYLTLSDATAALDWYKKVFLAEVPE
                     LHYAQDGKRVMHATVLMAGGVVMICDEFPEFMGGKKQNPAVLGGSGVTVQVHLPNVDE
                     VWERAVAAGAKIEMPLADQFWGDRYGVIADPFGHRWALFTHKPKPQEIGQTW"
     gene            complement(5572..6882)
                     /gene="epmA"
                     /locus_tag="PKL17_11360"
     CDS             complement(5572..6882)
                     /gene="epmA"
                     /locus_tag="PKL17_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNK45376.1"
                     /translation="MSASLRGRLIEVSARSLLVDTGDSMWRLTVACDVSSLCAGDFIA
                     VHADPVSVTDSIGVAQNVTLLARPQGTGQPFPSPGGDYFRLNCDRRRHLLSLRALALA
                     SIRNYFAAHDFLEIEAPLIVPSPGLELHLQGFSVAASDGKGPPRYLITSPEYQLKRLL
                     TSGLTRIYSLGKVFRSGESGPFHNPEFTMLEFYRAYEGWQSVATDVAALAERLATEIH
                     GSPRFVRHVEGIPQTIDLSLPWQTHTVASVVKTHAGIDLRGDETVDELRDKIAAAGFR
                     IPSPSRTDSAGNPYYAWDDLFFSMFLDQVEPKLMHAGEDGIHRPIVLCDWPAPLCALA
                     RKRPDNPAVVERFEAYVAGLELCNGFGELCDPVEQRQRFLKDQQERRVRDLPQYPLDE
                     RFLSALAEGMPPAGGVALGIDRLLMLILDAPHIRDVLAFGVDEL"
     gene            complement(6889..7464)
                     /gene="efp"
                     /locus_tag="PKL17_11365"
     CDS             complement(6889..7464)
                     /gene="efp"
                     /locus_tag="PKL17_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNK45377.1"
                     /translation="MANTYDTSDLRKGLKIMIDGAPYIVVEAQFVKPGKGQAFTRTRM
                     KNLLTGGVIERNIRSGEKMDGADVEDRESTYLYKEGDDSFVFMDKQSYDQVSVPSSTV
                     GDDWQFMKENTDVSLTFYNGRVISVTLPNFVQLRVTHSEPGVKGDTANNVTKPATLET
                     GATIAVPIFINEGDLLKIDTREGIYLERMRG"
     gene            7652..8761
                     /locus_tag="PKL17_11370"
     CDS             7652..8761
                     /locus_tag="PKL17_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNK45378.1"
                     /translation="MRTRAAVAYEAGKPLVIEEVDLEGPKAGEVLIEIKATGVCHTDE
                     FTRSGADPEGLFPVIFGHEGAGVVVECGPGVTTLKPGDHVIPLYTPECRGCKSCLSRK
                     TNLCTAIRATQGKGLMPDGTSRFSIKGKRIHHYMGCSTFANHTVLPEIALAKVRPDAP
                     FDKICYIGCGVTTGIGAVLYTAKVEAGANVVVFGLGGIGLNVVQGAKMVGADKIIGVD
                     INPAREPIARSFGLTHFVNPTQVKGDLVAHLVELTGGGADYSFECVGSVQLMRQALEC
                     CHRGWGVSVIIGVAGAGQEIATRPFQLVTGRVWKGSAFGGARGRTDVPRIVDWYMDHK
                     IAIDPMITHKLPLERINEAFDLMHAGASIRTVIEF"
     gene            8762..9613
                     /gene="fghA"
                     /locus_tag="PKL17_11375"
     CDS             8762..9613
                     /gene="fghA"
                     /locus_tag="PKL17_11375"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNK45379.1"
                     /translation="MTAPIQIRSEQKCFGGVQGFYEHTSTACSGPMRFAVYLPPGAGD
                     GNRIPALYYLAGLTCTEETFVVKAGAQRLCADLGIALVTCDTSPRAARFAGDDERWDF
                     GQGAGFYVDATEQPWSSAYRMHSYVVHELRDIVETSFPIARDRRGIFGHSMGGHGALT
                     IALTHPDLYASCSAFAPICAPSQVPWGELAFSRYLGSDKQAWRSYDACALIEEGKRFA
                     GEILVDQGLADKFLSRELRPELLSAACEATGQKLKLRRHDGYDHSYYFIATFLSDHLQ
                     HHARALR"
     gene            complement(9614..10561)
                     /locus_tag="PKL17_11380"
     CDS             complement(9614..10561)
                     /locus_tag="PKL17_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNK45380.1"
                     /translation="MLEARDLHLLVTFASVVRHGSFTTAAQELGQSKSLVSENIRCLE
                     ERCGVQLIERTTRRMRLTQVGEQVLSTATQIVNATRGLDALLEEHRSSPVGTLRVATT
                     HDLGSLLVAPLCAKLALLHPQLGIDIVTDDVSHDLIANRVDLAVRLGQPKESGNVVKP
                     LALVSEPIVAAPPLASQWSHVVRPRDLEGAPWARHSLVSRSDTLTFLGPKGEKEDVKL
                     QLRAQCNTGEGVRALLLQGIGIGVLPEYLIADDLQRGTLLRLCPGWIWKQVQLYVELA
                     QAKRKPRRVQLFLQALSDALTPNRTDGTIPLWQRLRRPG"
     gene            10697..11119
                     /locus_tag="PKL17_11385"
     CDS             10697..11119
                     /locus_tag="PKL17_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNK45381.1"
                     /translation="MESQKAKEIALALITPFYDSLTAPGQKPVADNIRSVTSEGWRSY
                     ASETVSKGREEFIMQVQGFGKILPDLHWDIKEVLVAGNRIIVRSEASGTPAGDFMGVP
                     HSGKSFRIMAIDIHTVENGKLVMAHHVEDWASGLRQLK"
     gene            complement(11180..12658)
                     /locus_tag="PKL17_11390"
     CDS             complement(11180..12658)
                     /locus_tag="PKL17_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK45382.1"
                     /translation="MTLRKIFTVGTFAVLSLALAHCDGSGTSGNNVDQGSTQDMAMPP
                     PVITSVAPGAVSNGGGVSITITGTGFQTGATVTVAGVACANPVVTATTITCTTGATTS
                     PVCGPTAIVVTNPDQQTTSNNTALQLKSKSLGFGNPSNIANVANDPNSKSAATADLNG
                     DGNMDVVNLDEVAGGAGVVPKLSIHLGKGDGTFLATTVVNLPATSNPTSLAVGDITGD
                     NKLDVVIAYGGTDQVQVFSGDGQGGFTGQTAVNVAAKNPISIALGDINADGKLDAVIG
                     HLAGNGLTTLTNTSGVLGGGTSINLGGMTITQISSVAVADVNADSKPDVVVTGATSNN
                     IAVFLGQGAAKFGAPTVFTMPGGPARLVLTDFNNDKKLDVATANAGAGTVSIRLGDAL
                     GGFGGTTPFTSIAAGMNSIALSAGDFDLDGNIDLVVANSTGSNFTALTGKGDGTLNAG
                     KTFGTNAGPSSIVSADYNRDGSLDVVTTSSTPNLAINLNVCQ"
     gene            complement(12756..14192)
                     /locus_tag="PKL17_11395"
     CDS             complement(12756..14192)
                     /locus_tag="PKL17_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK45383.1"
                     /translation="MLRRSYPLAVLAFSALALFGCDGAATDGSGDVADMGADDGGGGG
                     GPDLAPPYPAWNAQSPAICGQTPYTWQPASKVGTVLDYSRNLLPTLKFVIQTLEVAAF
                     LGSQLNVHHSVNYDVQASTVRYQTQDRGQLVDATAMVVWPKASGKTFPVLLFLHPTLG
                     YTDNCAPSQSRTDLTAPMTIFSMIAASAGYVAVFPDYLNQRSMGMASKTVTPYLLMEP
                     TAIVSWDAVRAAQKYVAANESLTASNDVYIWGHSQGAQAVEYVTAMQPLYAPEYNVKA
                     AAAVSPPSDLPETAKQNFASTAPTYNLGEAIAYAWADYYDMSKLTTALLSPWDGMALT
                     ELKNYCTSGYTDPIKAVTDPAKIFTTPFLDALLRGQKYDPWSCWLYYNNPATMGPIMN
                     PKVPMLYVTGDKDTTVPPPANDPVTAKWCGQGVKIQYLQCAGADHVHSLTNSVDDVLK
                     FFEDRQAGLPMPADVCQSKPAVKCASTL"
     gene            complement(14321..15637)
                     /locus_tag="PKL17_11400"
     CDS             complement(14321..15637)
                     /locus_tag="PKL17_11400"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNK45384.1"
                     /translation="MKRSQRRLLLDATTRLRKLGLSLRQVFSGTVDEEAEKMQTERLE
                     AEELYRSAAGLRGGIAKIAQLRAYLQGASALGPEAQQVLGRLWDRAPTEQPQAIRQVV
                     ESELGQPISALFKEWNDEPLAAASLGQVHPAVSHDGILLAVKVQYPGVAEALADDLDS
                     TGVLRDLVGGDMGEALPVQSLKALREHLLAELDYRAEAEHLRRFARLFADDSHVVIPS
                     VIDHLSATKVLTMQRLVGQSLPTVARDGSDAQRSSIGQTILRFALVCPIRHGVINVDP
                     NPGNYIILDGSGDQAKVGFLDFGCVAEVPEELQKADRALYMSMIKRDGEALRYAAYLQ
                     GLIPSVSAFDNATYRRWEKLLCAPFMERDSVRLTPEYVKELGELTWMLLHSRKLQLPP
                     AALLLWRQRLGLLAVVSALCPALHFRALLADVLDDGHPIPMLQRYP"
     gene            complement(15683..17542)
                     /locus_tag="PKL17_11405"
     CDS             complement(15683..17542)
                     /locus_tag="PKL17_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45385.1"
                     /translation="MSDKKKTPEKSRAEQKLAAFHDEDNIDKSYDFQLLSRLWPFIKP
                     HARYVVLSLLALVAVAGINLLRPILMGKVVAHAGDKAADLLLRDGAILAGLVVLSQFV
                     SFAQIYSMQLAGAMSMADLRRHVFHFLQGLSMRFYDKTPVGRLTTRCTNDVDAVSELF
                     AFGVFNAVGDLLMLVGIVVAMLLLDWQLSLIAFAALPVVGALVTWVRRGGKRAYRDIR
                     TKTARLNAFLSEQVGGIAVVQAYGREEQMAREFDGINVQFRDANKRAIFFEAVLDAAI
                     EMVSTLCVASVLWWVGVQKLGSRPISFAMVVTFTQYLRQFFEPVSMLTQRYTVLQSAL
                     SGAERIFQLLDETDVEQIVAAESSASAVADEGIALDGIHFGYKPNVPVLRDVTLLARR
                     GEKLALVGATGAGKSTVTQLVLRLYQPSAGQVRVLGKDVRSYSPQELRRQFAVVPQDV
                     FLFQGTVLSNIAMGDASPDPERAQAALVRIGALEMIERRGGLQAIVDERGSNFSAGER
                     QLISFARAVYREAPILILDEATASVDSDTEARLQKALDAVMKDRTALVIAHRLSTIQA
                     ADRIVVFHKGRVVEQGRHEELLAHDGVYARLYRLQFAAKNPHSAENPQPVAVG"
     gene            complement(17539..>18410)
                     /locus_tag="PKL17_11410"
     CDS             complement(17539..>18410)
                     /locus_tag="PKL17_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45386.1"
                     /translation="SFNRLCEIIDAEPDLFDGKDHLPNPPRGELQVQGLSFRYGDREV
                     LSNVSFTLPAGGSLAIVGRTGSGKSTLAMLLARLIPTPPNTVRLDGKDVCDLPLREVR
                     STVGYAQQDAFLFSTTVARNIGLMLDEPDSPSSQQRLQQAAREAQILEEVQGLPEQLD
                     TIVGERGVQLSGGQKQRVALARALLWQPKLLILDDPLSAVDAKTESAILQSIEKQAVE
                     RSLLLITHRVAAAARCQHILVLDQGKVVESGTHEQLLAQGGIYATFAEEQRMASELDA
                     LGASDLPGSEVRP"
BASE COUNT         3625 a         5589 c         5488 g         3708 t
ORIGIN      
        1 ccgacttctt cggagcagcc gacttctttg gagcagccga cttcttggct gccggtttgc
       61 gggcgctcgc tggcttggct ggcgtcggag cgacctggcc atcgcgtttc acaccgtcga
      121 aaatggcccc tggaaagcgc gcgccggtca ggttggcggc gccaaagtct gcgccggtga
      181 ggtcggtgtg ttcaaaatcg gcaccgcgca gatccgcgcc aacaaacgaa cagccgcgca
      241 ggctcgagcc ggaaaagtcg actttgcgca gcgatgcacc gtcgaatgtt gcgccatcga
      301 agaacgcatc gaccgcggca caaccgtcaa ggatggcttt gccaaagtcg acgccgcggc
      361 agatgcttcc cgacaaatcg gcgtatccga tgtcggcacc gcgcagcgat tcaccggcgg
      421 gaatttgctt catccgcagc acaagctgcg caccgacttt gttttcgtcg ctctggccgt
      481 agacctgata gatgcacagc ggcagaccgc tcaccgacag aagctgccag tctccttcgc
      541 gatttccggc gtcgagaaac agcttgtgct gcgcaaccgc ggtcgcaagt tcttggccct
      601 taagcggcgt tcccgacgct gccaggcttt cgatgagctg cgggtccatc tgccgcgttc
      661 cgcgcggctt gtcggaaagc ggaaagtcaa ccagccacgg cttgacccac gcatcgggcg
      721 gggtttccag cgtcttggag cgcggcttgt tgacccaggg accgtttggg caccacgaag
      781 cggaatcaaa gcgcattcct ggctcgatgt gatcgatcca cttcggcgcc gtcaccgaaa
      841 ttcggtaggt cgccgacgga acccgcgctg gatcactcca gtacgcgcgc cattcgtttt
      901 cccaggccga ttcccagtcg tcgctgtcgc gggcaatgcc gcgctcgatc acccgcttgt
      961 caacgccggc gtgaacctgt ttttcgacga aaggaacgtt gctcgctttg tagacctgga
     1021 ctgagcgaac aaacttcttg gcgcggtctg cgcactcctc ggcgtcgtag tagttgtctt
     1081 cgctaaactc gctgctcagc ggactgggtg cccgtcgatt tcccgacgag tcccagccaa
     1141 acgcgggaat caaaagcagc tgcagcgcaa aaccggggga ctcgggaaac ggccccgcat
     1201 cgggatggtg cagggtgact ttcaaatcaa cccagtcctt gccgcggttc agaatgtcga
     1261 tgtcccacag atcagacatt gcggtctccc ttttcccccc ggttctccgc cttggctagt
     1321 tacgcagcgg cttgttgttt tgcagcatgt acatcatgcg gtcccggctc ttttgctcgc
     1381 cgcacagcgc aaggaactgc tcgcgttcca gatccagaag ctgctgctcg gtcatgcgca
     1441 ccgttggcga cgtattgcca ccggtcagca ctttggcgag cgcgctgccg acggtgaggt
     1501 catgcggcat gatctggtgc gcttccagca tcatctgcaa gctggatcga atgttggcgt
     1561 aacccgacgg gcccggcagt cgaaccaagc gctgacgcgg cgggcgatag ccggcgcgca
     1621 gaagacccag cgcaagctgc ttggcgtcgt aggtcaggtg atcacggttg aacgtcacgc
     1681 cgtctgtctc acgcaggaag ccgaggctgc gcgcttcttc cgccgaggtc gcgaccttgg
     1741 caagaccaat cgtctcaaag gcccgctgca ccgaggggaa cggatcggcg ttctcgggca
     1801 ggtgtgactg caggcggcag atcagctctt tgcagccacc accaccgggg agcagaccga
     1861 cgcccacttc cacaagaccc atgtacagct cggcgtgggc gcgcaccgcg tcggcgtgca
     1921 tcgtgacttc ggcgccaccg cccagcgtca gtgcaaacgg agccaccacg acgggcaccg
     1981 gtccgtagcg caggagctgg ttggcatttt ggaagtccgc gacgactttt tcgagcatcg
     2041 tccagttgcc ctgcatcgcc gccatcacga tcagcatcaa gttcgcgcca gccgaaaacg
     2101 aatcggtggc ttcgttggtg atgacgagag cttttttgcg cacgtcgcag atttcgatcg
     2161 ccttgttcag caggccaatc acatccgcgt cgatcgagtt catcttggtc aaaaactcaa
     2221 gcgcgtagac gtcgtcgtgc aggtcgataa gccgcgcgcc atcgtttttg tcgatgacaa
     2281 gaccctgcga cgaaagaccg gcgagagaaa gctcacgcgg cgacttcgga accgccactt
     2341 cagccttggc cgacagatcg aagtagtgtt tctgacccgg ctcgccgctg taaaacgaac
     2401 tgcggcccgc gctcagcatg ccgtacaccc actgggccgg acggatgccg ctttgttcca
     2461 tgcgctcgac gcttgccttc acaccgacgg cgtcccacat ctcgaacggg cccatttccc
     2521 agccgtaccc ccagcgcatc gcgcgatcga cttggcacag atcgtcggcg atgggcgcgt
     2581 cgttctggtt gccatcgact tcgctgcgcg gtccgatgcg gcgcgacgaa tacgaaaaga
     2641 gcagggcggt cgctttccac gccagctccg cggcacggtc ttggcccgaa agcaccgaac
     2701 gaattcgctc ggcgacatcg gtcaggctct tgccggcgga cagcgactcg aaacgggctt
     2761 tctgctgggc gcggtattcg agcgtgttga ggtcgagcac caaaaggcct tccttggtct
     2821 tttggtaaaa gcccttgccg gtcttgtcgc ctttgtaacc gcgacggacc atttcccgga
     2881 tgacgtcggg ggtggcgaag acttggcggg cttcgtcgtg gatcaggttg tcgtagcagt
     2941 tttgcgcgac gtcgatgacg gtgtcgagtc cgaccatgtc agcggtgcgg aacacggcgc
     3001 tcttggggcg accgagcggc ggaccaaaga tcgcgtcgac ttcttcgacg gtgaagccgg
     3061 ctttcagggc ttccgaaatc aggcccatca tgccgtaaat gccgatgcgg ttggcgacga
     3121 agttggtggt gtctttgccg tagacgatgc ccttgccaag ctgggtttcg ccaaagccgg
     3181 cgatctgaca catcacctgc ggatcggtgt cttcgcccga gaccagctcg aggagccgca
     3241 tgtagcgcac cgggttgaaa aagtgcgtga cgaggaagcg acggcgcata tcgagcgcgc
     3301 gaccggccat cagcttgcca atcggcagac ccgacgtatt cgacgccagc accgtctgcg
     3361 ccccgagcag cggttccagc ttggcaaaca gcgcttgctt gacgttcaag tcctcacgaa
     3421 cagcctcgat gacgatgtcg gctttggcgg cttccgccag atcatcttcg aggttgccga
     3481 cgcgaatccg ttccaggtcg cgctggtgaa aaatcagcga cggcttcgat gtccgcagct
     3541 tgtcgacgcc accctgcgca aagcggttcc gcgctttttt gtcggcgttc tgcgccgtcg
     3601 ccagatccgg cggcacgatg tcgaggagga gcacgtccag acctgcgccg gccagatgtg
     3661 ccgcaattcc agaacccatc acgcccgcgc cgagaaccgc ggccttccga aagcgccgat
     3721 cctgcttacc ctgcttgtcc atcctgatat tcctttcgag tcgttggccg ggagtctatc
     3781 accaatcgcg ggattttccg ttgttgacag cggtaggcgc gctttgcaaa agtggcaggc
     3841 cgtacatttc aagcacctac acggaggatt ttgctcatgg gtcgaggaga caatcgcaac
     3901 agcccgaaaa tgcgtcgcaa gaaagcgcaa acgcggctga agaaccgcat caagaaccgt
     3961 cgcgtcgctg ctgccgcggt ccgtgccgaa aagaaggccg ctaccgctgc tgcgcccgcc
     4021 gcaaagcccg ccaaaaaagt cgcccactag tccgggcgtt ttccgctttt ttgcggcatt
     4081 cattcgtcag gcacgcccac ccctgctttt tcgcagaaca tcgtgatacg gtcttttttg
     4141 ggggacttat ccgatgggct ggcgtgccga ttacgttgca agtcatggct ttgtcgagac
     4201 gcactacgaa ggtgtgctga gtcgagaaga ccttggcgct gcaattgagc gcacgctggc
     4261 gctttcgcgt gagcacgaca cgacgctgtt tttgggcgac tgctcgcggc tcgaaggtgg
     4321 acacgacatc ttcacgctct atggctttgt gcgacttttg gaatcgctcg ggctggtcgg
     4381 tcgcttcaaa gaagcgctga tcatgcccac gctcgcagca ccagcgcgcg atgtggagtt
     4441 ctgggagact gcgtgcctca atcgcgggct gctcgtgcgg gtctttccag atcgcgaaag
     4501 cgcgtgtgac tggctctgcc agcggcctta ttttgtgcag cctttgaatc cggcctagcg
     4561 gacaggctgg ccaaaatcca ggttcaactc gcgcccgcgc tctgcgatac tgacgtgggg
     4621 ggcacgatgt cagggggctg gcaatggctc ggaatccttt tgcttttgca gggtggctgc
     4681 gtgccgcagg cggtctattt ggccaaagca gaggaagtcg agcagctcaa gagccgcgaa
     4741 cagcttgtgc agcagcgctt gggtgaacga atcgatcggc tgtcagcgcg actttccgat
     4801 ctggaaaaag agatcggaaa gaccgagccg cgctggactg agctgtctgc ggcgatgcag
     4861 cttgtgcggg atctggcggt gcagacagat cagaatctgc tcaagctgct tgtgcagcgt
     4921 gacacgcgct gtgactgccc aagcgcgccg ccaaagacgc cgccaccatt cgggcgtgga
     4981 cagaaaacgc cgccgctgtt tccctcgaag cgccactaag cgctaccagg tctgtccgat
     5041 ctcttgtggt ttgggcttgt gcgtaaacag cgcccagcga tggccaaacg gatcggcaat
     5101 gactccgtat cgatcacccc agaactgatc cgcgagcggc atctcaattt ttgcgcccgc
     5161 agcgacggct ctttcccaaa cttcatcgac attgggaaga tgaacttgca ccgtcacacc
     5221 cgatcctccg agcacagcag gattctgttt tttgccgccc ataaactccg gaaactcatc
     5281 gcagatcatg acgactccgc cagccatcag caccgtcgca tgcatgactc gttttccgtc
     5341 ttgggcgtag tgcagctcgg gtacttctgc caaaaagact tttttgtacc aatcgagcgc
     5401 tgcggttgca tcggacagcg tcaggtacgg aatgatcggc ggatactgga agtcatcgtg
     5461 gctgtgttcg gcttgggttt cggacatgag cggactcctt tcgatcagga attgtgcgcc
     5521 cacagtttgc cgaccacgcg cggctgtcaa cagaatctgc gcttacgttc gctataattc
     5581 atcgacgcca aacgccagta cgtcgcgaat gtgtggcgca tccaaaatca gcatcaggag
     5641 cctgtcaata ccgagcgcta caccaccggc tggaggcatt ccttccgcca gtgcagaaag
     5701 gaatcgttca tccaggggat actgtggcag atcacggact ctgcgctctt gctgatcctt
     5761 taggaatcgc tgccgctgct ctactggatc gcacagctca ccaaagccgt tgcacagctc
     5821 cagtccggct acgtaggctt cgaatcgctc gacgacagcg gggttgtcgg gacgcttgcg
     5881 cgccagtgca cagagcggcg ctggccagtc acacaagacg atcggacggt ggattccgtc
     5941 ttcgcccgcg tgcatcagct tgggttcgac ctgatcgagg aacatcgaaa agaacagatc
     6001 atcccaagcg tagtaaggat tccctgcgga atcggtgcgc gaaggactgg gaatccgaaa
     6061 accagccgcc gcgatcttgt cgcgcagctc atcgaccgtt tcatcgccac gcagatcaat
     6121 tccggcgtgc gttttgacga cagaagcgac tgtatgggtc tgccacggaa gcgaaagatc
     6181 gatggtttgg gggattcctt cgacatgacg aacaaagcgc ggagagccgt gaatttccgt
     6241 cgccaaacgt tctgcaaggg ccgcaacatc ggtggctacg gactgccagc cttcgtaggc
     6301 gcgataaaac tccagcattg taaattctgg attgtgaaag gggcccgatt caccgctgcg
     6361 gaacactttt cccaagctat aaatccgcgt aagaccactc gtgaggagtc gcttgagctg
     6421 atattccgga ctggtaatca gataacgcgg cggacctttg ccatcggaag ctgcgactga
     6481 aaacccttgc aggtgcagct ccagtccggg ggacggaacg atgagcgggg cttcaatttc
     6541 cagaaagtcg tgcgcggcaa aatagttgcg aatcgaggcc agcgcgagcg cccgcagcga
     6601 caaaagatgg cgacgacgat cgcagtttaa gcgaaagtag tccccaccgg gcgaagggaa
     6661 gggctggccg gttccttgcg gacgggcaag cagcgtcacg ttctgtgcaa cgccaatcga
     6721 atcggtcaca gagaccggat cggcatgcac cgcgatgaag tcccccgcgc acagggaaga
     6781 gacatcacac gcgacggtga gccgccacat ggaatccccc gtatccacaa gcagcgagcg
     6841 cgcggacacc tcgatgagac gaccgcgcag cgaagcagac atcggaaatt acccgcgcat
     6901 acgctcgagg tagatgcctt cgcgggtgtc gatcttcagc aaatcgccct cgttgatgaa
     6961 gatgggcact gcgatggtcg caccggtttc cagcgtcgcg ggcttggtga cgttgttggc
     7021 ggtgtcgcct ttgacgcccg gctcgctgtg ggtgacgcgc agctggacga agtttggcag
     7081 cgtgaccgaa atgacgcgac cgttataaaa cgtcagcgaa acgtcggtgt tttctttcat
     7141 aaactgccag tcgtcgccga cggtggacga cggaacgctg acctgatcat acgactgctt
     7201 gtccatgaac acgaagctgt cgtcgccttc tttgtagagg tacgtcgatt cgcgatcttc
     7261 gacgtcggcg ccgtccattt tttcaccgga gcgaatgttg cgctcgatga cgccgccggt
     7321 caggaggttc ttcatgcggg tgcgggtgaa cgcctggccc ttgcccggct tgacgaactg
     7381 cgcttcgacg acgatgtagg gagcgccgtc aatcatgatc ttcagaccct tgcgcaggtc
     7441 ggacgtatcg taggtattcg ccatgtttgc ctcagagtaa aacggatcgg ggccttttta
     7501 gctgcagcgc aacctgtgac ccgagcgcgg tggttttact tcggctttgt cgcgcggtca
     7561 actacccaaa aagccagcgg ctcgcccttg tcagagacga gctttgcttg ctaacgtgcc
     7621 gcgtgtatca aattatccgg ggagccaagt catgcgtaca cgcgccgctg ttgcttacga
     7681 ggctgggaag ccactggtca ttgaagaagt cgatctcgaa ggcccgaaag cgggtgaagt
     7741 tctgatcgaa atcaaagcca ccggagtctg tcacaccgac gaattcactc gatcgggcgc
     7801 agatccagaa ggtctgtttc cggtgatttt cggacacgaa ggcgccggcg tggtggtcga
     7861 atgcggaccc ggcgtaacga cgctcaagcc cggcgaccat gtgattccgc tttacacccc
     7921 cgagtgtcgc ggctgcaagt cgtgtctgtc gcgcaaaacc aatctttgta cagcgattcg
     7981 cgcgacccaa ggcaaagggc ttatgcccga cggaacgagc cgtttttcga tcaaaggaaa
     8041 acggattcac cactacatgg gctgctcgac gtttgccaat cacaccgtcc tgcctgaaat
     8101 tgcgctggcc aaggttcgtc ccgacgcgcc gtttgacaaa atctgttaca tcggctgcgg
     8161 cgtcacgacg ggaatcggtg cggttcttta caccgcgaaa gtcgaagccg gtgcaaatgt
     8221 cgtcgtgttc ggactcgggg gcattggtct aaacgtcgtg caaggcgcca aaatggtcgg
     8281 tgccgacaag atcattggcg tcgacataaa ccccgcgcgt gagccgatcg ctcgcagctt
     8341 tggcctgacc cactttgtca atccaaccca ggtgaaaggc gacttggtgg cgcatctggt
     8401 cgagctgacc ggcggcggcg ccgattacag cttcgagtgc gtcggcagtg ttcagctgat
     8461 gcggcaagcg ctcgagtgtt gccaccgcgg ctggggcgtg tcggtcatca tcggcgtggc
     8521 gggcgcgggt caagagattg cgacacgtcc cttccagctt gtcaccggac gggtctggaa
     8581 aggatcggcg tttggtggcg cacgcggtcg cacggatgtg ccgcgcatcg tcgattggta
     8641 catggatcac aagattgcga tcgatccgat gatcacccac aagctgccgc tcgaaaggat
     8701 caacgaagcg tttgatctga tgcatgccgg cgcgtcgatc cgcacggtga ttgagttcta
     8761 aatgacggct cccatccaga tccgcagcga gcaaaagtgc tttggcggag tgcagggttt
     8821 ttacgagcac acaagtacag cgtgttcagg tccgatgcgt tttgcggtgt atctgccgcc
     8881 gggcgcaggt gatgggaatc ggattccggc gctctactat ttggcggggc tcacctgcac
     8941 ggaagagacg tttgtggtga aagccggggc gcagcgtctg tgcgcggact tgggaatcgc
     9001 gctggtgacg tgtgatacaa gtccacgcgc cgcgcgtttt gcgggcgatg atgagcgctg
     9061 ggactttggc caaggtgctg gcttttacgt cgacgcgaca gagcagccgt ggtcttcggc
     9121 ctaccgcatg cattcctatg tggtgcacga gctgcgggac atcgtcgaaa ccagctttcc
     9181 gattgcgcgt gaccggcgcg gaattttcgg gcactcgatg ggtggtcacg gcgcgctgac
     9241 gattgcgctt acacaccccg atctgtatgc aagctgctcg gcgtttgcgc ccatctgtgc
     9301 tccgtcgcag gttccgtggg gagagctggc attttcgcgc tatctgggca gcgacaagca
     9361 ggcgtggcgc agctacgatg cgtgcgccct catcgaagag ggaaagcgat ttgccggcga
     9421 aatcctggtc gatcaaggac tggccgacaa gttcctttcc cgcgagctgc ggcccgagct
     9481 gctttcggcg gcgtgcgaag cgactggaca gaaactgaag ctgcgacggc acgacggcta
     9541 cgatcacagc tactacttca tcgcgacctt cctttccgat cacctgcaac accacgctcg
     9601 cgcgctgcgc taactatccg gggcgacgga gtctctgcca cagcggaatg gttccgtccg
     9661 ttcgattggg tgtcagggca tcggacagcg cttgcagaaa cagctggacg cgacgcggtt
     9721 tccgcttcgc ttgcgcgagc tccacataaa gctgtacctg tttccaaatc cagcccggac
     9781 acagccgcaa aagcgttccg cgctgcaagt cgtcggcgat cagatattcg ggcagcacgc
     9841 cgatgccgat tccttgcagc agcagagccc ggactccttc cccggtgttg cactgcgcgc
     9901 ggagctgtag cttgacgtct tccttttccc cttttggacc caaaaacgtc agtgtgtcac
     9961 tgcgtgaaac cagcgaatga cgcgcccacg gagcgccttc cagatcgcgc ggacgcacca
    10021 catgcgacca ctggctggcc aagggcggcg cggcgacaat cggttcggac accagcgcca
    10081 gcggcttgac cacgttgccc gattccttgg gctgtcccag ccgtaccgcc agatcgacgc
    10141 ggttcgcgat caagtcgtgt gacacatcat cggtcacgat gtcgatgcca agctgcggat
    10201 gcaaaagcgc cagcttggcg cacagcggcg cgaccagaag cgatcccaga tcgtgcgttg
    10261 tcgcaacccg cagcgttccc acgggcgagc tgcggtgttc ttccaacagt gcatccagac
    10321 cgcgcgttgc attcacaatt tgcgtcgctg tgctgagcac ttgttccccc acttgggtca
    10381 gccgcatccg tcgcgtcgtc cgctcgatga gctgcacccc acagcgctct tccaggcagc
    10441 gaatgttttc gctgaccagg ctcttggact gtcccagctc ttgcgccgcc gtggtgaacg
    10501 atccatgccg caccaccgac gcaaaagtca ccagcagatg cagatcccgc gcttcaagca
    10561 ttgttcgact ttaccgaaca gactttcaag atcaagcccg attgtttaca aatccagctt
    10621 gctgcatctt ggtttcatcg ctgctgcacg ggggtctgtg cagcaaaacg gaaatcctac
    10681 ggaaagaggt atgtccatgg aatcgcaaaa ggccaaagag atcgcgctcg ccctcatcac
    10741 tccgttttat gattcgctca ccgcgccagg ccagaagccc gtcgctgaca acattcgcag
    10801 cgtgacttcg gagggctggc gttcgtatgc cagtgaaaca gtcagcaaag gccgcgagga
    10861 gttcatcatg caggtgcagg ggttcgggaa aatccttccg gatctgcact gggacatcaa
    10921 agaagtgctg gtcgccggca atcggatcat cgtgcgcagc gaggcgagcg gcacaccagc
    10981 cggtgacttt atgggcgttc cccacagtgg caagagcttc cgcatcatgg cgattgacat
    11041 ccacaccgtc gaaaatggca agctcgtgat ggcgcaccac gtcgaagact gggcatccgg
    11101 tctgcgtcag ctcaagtagt atcgggcagg ggttcgaagc aaaccgacgg aaaagtcggc
    11161 ttgcgtacaa gcgggcggat tactggcaga cgttcaggtt gatcgcgaga ttgggcgtgc
    11221 tgctggtcgt taccacgtcg agcgagccgt cgcggttgta gtccgcagac acaatgctgc
    11281 tgggccccgc gttggtgcca aacgtcttgc ccgcgttcag tgtgccgtcg cctttgccgg
    11341 tcagcgcggt gaagttgctc ccggtgctgt tggccaccac gaggtcgatg ttgccgtcga
    11401 ggtcgaagtc gcctgcgctc agcgcgatcg agttcattcc cgctgcaatc gatgtaaacg
    11461 gcgtcgtgcc accgaaccca ccgagcgcat caccgagccg gatgctgacc gtgccagccc
    11521 ctgcgttcgc cgtcgccaca tcgagctttt tgtcattgtt gaagtcggtc agcaccaggc
    11581 gggccgggcc tccgggcatg gtaaacaccg tcggcgcgcc aaacttcgcc gcgccctgac
    11641 ccagaaacac cgcgatgttg ttgctggtcg cgcccgtgac cacgacatcc ggcttgctat
    11701 cggcgttcac gtcggcaacg gccacgctcg aaatttgcgt gatcgtcatg ccgcccaggt
    11761 tgatcgatgt accgcctccc agcacgccgc tcgtgttggt cagcgtcgtc agtccgtttc
    11821 cggccaggtg tccgatcacg gcgtcgagct tgccatcggc gttgatgtcg ccaagcgcaa
    11881 tcgagatcgg gtttttcgcc gcgacgttga ccgcagtctg tccggtaaaa ccgccttggc
    11941 cgtcgccaga aaagacctgg acttgatcgg ttccgccata cgcaatcacc acgtcgagct
    12001 tgttgtcgcc ggtgatatcg cccaccgcga gcgaggtcgg attcgatgtg gcgggcaggt
    12061 tgacgaccgt ggtggccaag aaggtgccgt cgcctttgcc caggtggatg ctcagcttgg
    12121 gaacgacacc cgcgccgccg gccacttcat ccaagttcac cacgtccatg ttgccatcgc
    12181 cattgaggtc tgccgtcgct gccgacttgc tgttgggatc gtttgcaacg ttggcgatgt
    12241 tggacggatt gccaaagccg agcgacttgc tcttaagctg cagcgccgtg ttgttgctgg
    12301 tcgtttgctg atcaggattg gtgacgacaa tcgccgtcgg tccacagacc ggggatgtcg
    12361 tggcgccggt cgtgcaagtg atcgtcgtgg cggtcacaac gggatttgcg caggcgacgc
    12421 ccgcgacggt gacggtggcg ccggtttgga atccggtgcc ggtgatggtg atgcttacac
    12481 cgccgccgtt tgagacggcg cctggtgcga cgctggtgat gaccggtggt ggcatcgcca
    12541 tgtcttgcgt gctgccctga tcgacgttgt tgccgctggt tccgctgcca tcacaatggg
    12601 ccagagccag cgaaagcacc gcgaaagttc ccaccgtaaa aatcttgcgt agcgtcatgt
    12661 ggacattccc cctttagtct aaggacgccg attcgacgat tttcgccgtc ggcaagttcc
    12721 accggaaaga ctccggcggt ttcgtaagca atttgctaaa gcgtgcttgc gcacttgacc
    12781 gcaggtttgc tttgacagac atctgcgggc atcggcagac cggcttgccg atcttcaaaa
    12841 aacttcagta cgtcgtcgac cgagttggta agtgagtgca catggtcggc cccggcgcac
    12901 tgaagatatt ggattttcac gccctggccg caccacttcg ccgtgaccgg atcattggcg
    12961 ggaggcggaa ctgtggtgtc tttgtctccc gttacgtaca gcatcgggac tttgggattc
    13021 atgatcggtc ccatcgtcgc tggattgttg tagtaaagcc aacagctcca cggatcgtat
    13081 ttttgtccgc gcagcagcgc atcgagaaac ggcgtcgtaa aaattttcgc cggatcggtc
    13141 actgctttga tcggatcggt gtagccgctg gtgcagtagt ttttcagttc cgtcagcgcc
    13201 atcccatccc acggggacaa aagcgcagtc gttagcttgc tcatgtcgta gtaatccgcc
    13261 cacgcatagg caatcgcttc gcccaagttg tacgtcggtg cggtgctggc aaagttctgt
    13321 ttcgcggttt ccggaagatc gctcggtggg gatacggcgg ctgccgcttt gacgttatat
    13381 tccggcgcgt acagaggctg catggcggtg acatactcga cggcctgcgc gccttgggaa
    13441 tgaccccaga tgtacacatc gtttgacgcc gtcagcgatt cattggccgc gacatacttt
    13501 tgggccgcgc gcaccgcatc ccaagaaaca atcgccgtcg gttccatcag cagataaggg
    13561 gtcacggtct tcgatgccat tcccatgctg cgctgattca ggtagtcggg aaacaccgcg
    13621 acgtatcccg ccgacgcggc gatcatcgaa aagatcgtca tcggtgctgt cagatcggtg
    13681 cgactttggc tcggggcaca gttgtcggta tagccgagcg tcggatgcag aaacaaaagc
    13741 accggaaagg tcttgcccga cgctttgggc cacaccacca tcgcggttgc atcgacaagc
    13801 tgaccgcgat cctgcgtttg atagcggacc gtcgatgcct gtacgtcgta gtttaccgag
    13861 tgatggacgt tcagttggga accaagaaac gccgccactt cgagcgtctg aatcacaaac
    13921 ttgagcgtcg gtagcagatt gcgcgaataa tccagcacgg tgccgacttt tgacgcgggc
    13981 tgccaggtgt acggggtctg tccgcagatc gccggacttt gcgcattcca ggccgggtag
    14041 ggcggtgcaa ggtcgggtcc accaccgcct ccaccatcat cagcgcccat gtcggcgaca
    14101 tcgccactgc cgtcagtcgc agctccatca cagccaaaga gtgccagcgc agaaaaggcc
    14161 aagaccgcaa gcggatacga cctacgcaac atgtgtcccc ccagcgtaaa agaacatctt
    14221 aacacccaaa cgcgagcggg tcttttcgct gggcgatgga tgggaatcgc gccgtctgaa
    14281 actgggaggg aacggctgtt aggcggtcgc gctggcggtt ttacggatag cgctggagca
    14341 tgggaatcgg gtgtccgtca tcgagcacgt ctgccagcag cgcgcgaaag tgcagcgccg
    14401 gacacagcgc cgaaaccacc gccaaaagac cgagccgctg tcgccacaaa agcagggccg
    14461 caggtggaag ctgaagcttg cgactgtgca aaagcatcca cgtcagctcg cccaactctt
    14521 tgacatattc gggtgtaagc cgcaccgaat cacgttccat aaacggcgcg cagagcagct
    14581 tttcccagcg ccgatacgtc gcgttgtcaa atgcgctcac cgacggaatc agcccttgca
    14641 gataggcggc gtaacgcagc gcttcgccat cgcgcttgat catcgacatg tagagcgcgc
    14701 gatcggcctt ttgcagttct tcgggcactt ctgccacaca gccaaagtcg agaaaaccga
    14761 cctttgcctg atcgccgctt ccatcgagga tgatgtaatt tccggggttt ggatcgacgt
    14821 ttatgacccc gtgacggatc ggacatacca gcgcaaaacg caagatggtc tgtcctatcg
    14881 agctgcgctg ggcatcgctt ccatcgcgtg ccaccgtcgg caaggactgt cccacaagcc
    14941 gctgcatggt caggactttg gtcgccgaaa ggtggtcgat gacgctggga atcacaacat
    15001 gggaatcgtc agcaaacagt cgagcaaagc gacgcagatg ctctgcttcg gcgcggtagt
    15061 cgagttccgc caaaagatgc tcgcgcaggg ctttcaggga ctgtacgggc agcgcttcgc
    15121 ccatatcgcc accgaccaga tcgcgcaaaa cccccgtcga gtccaggtca tcggcgagcg
    15181 cttctgcgac ccccggatat tgcaccttta ccgcaagtaa tattccatcg tgtgaaaccg
    15241 ccggatgcac ctggccaagc gacgctgcgg cgagcggttc atcattccat tccttgaaaa
    15301 gagcggatat cggttgcccc agctctgact cgacgacttg ccggatggct tgcggctgtt
    15361 ccgttggtgc gcgatcccac agccgaccca gaacttgctg cgcttccggt cccagcgccg
    15421 atgccccctg aagataggcg cgcagctggg cgatcttggc gatgccgccg cgcagaccgg
    15481 ctgcggatcg atacagctcc tcggcttcga gccgctcggt ctgcattttt tctgcttctt
    15541 catcgacggt gccgctaaac acctggcgca gtgacagacc cagcttgcgc agccgcgtcg
    15601 tcgcatccag aagcaaacgt ctttgggaac gcttcatggc aaacctgttt tcggtaacag
    15661 tcgaggagcc gccgatgttt cgctagccca ccgcgacggg ctgggggttt tcggcgctgt
    15721 gcgggttttt ggccgcaaac tggagccgat acagccgggc atatacaccg tcgtgcgcga
    15781 gcagctcttc gtgacggcct tgctcgacga cgcggccttt gtgaaagaca acgattcgat
    15841 cggcggcctg aatcgtcgac agccggtgcg caatcacaag cgccgtgcgg tctttcatca
    15901 ccgcgtcgag cgctttttgc agccgcgctt cggtatcgga atcaacgctc gctgtcgctt
    15961 cgtcgaggat caggatgggg gcctcacggt agaccgcccg cgcaaacgaa atcagctgtc
    16021 gctcacccgc cgaaaagttc gatccacgct cgtcgacaat cgcttgcaga ccgccgcgcc
    16081 gttcgatcat ttcgagcgct ccgatgcgca caagcgccgc ctgagcccgc tcaggatcgg
    16141 gactcgcatc gcccatcgca atgttggaca gcaccgtgcc ttggaacaaa aagacatcct
    16201 gcggcaccac cgcgaactga cgacgcagct cttgcggaga atagctgcgg acatctttgc
    16261 ccaagacccg gacttgcccc gccgacggct gatatagccg cagcacaagc tgggtcactg
    16321 tgcttttgcc agcgccggtt gccccgacga gcgccagctt ttcaccgcgc cgcgcgagca
    16381 gcgtcacatc ccgcagcacc ggtacgttgg gcttgtaacc aaagtgtatc ccgtcgagcg
    16441 caatcccttc atcggccact gccgacgcgc tggattccgc cgccacgatt tgttccacat
    16501 cggtttcatc cagaagctgg aaaatccgct cggcccccga cagtgcgctc tgcagcacgg
    16561 tgtagcgctg cgtcagcatg ctgaccggct cgaagaactg ccgcaggtac tgggtaaacg
    16621 tcaccaccat cgcaaatgaa atgggccgcg agccaagctt ttgcacgccg acccaccaca
    16681 gcacgctggc cacacaaagc gtcgacacca tttcaatcgc cgcgtcgagc accgcttcaa
    16741 agaaaatcgc ccgcttgttg gcgtcgcgaa actgcacgtt gatgccgtcg aactcgcgcg
    16801 ccatctgttc ttcgcgaccg taggcctgta ccaccgcgat gccaccgacc tgttcggaca
    16861 aaaacgcatt gagccgcgcc gtcttggtcc gaatgtcgcg atacgcccgc ttgccgccgc
    16921 gccgcaccca cgtcaccagc gcaccgacga ccggcagcgc tgcaaacgca atcagcgaaa
    16981 gctgccagtc aagcagcagc atcgcgacca cgattccgac cagcatcagc aagtcaccca
    17041 cggcattgaa gacgccgaag gcaaacagct ccgacaccgc atcgacatca tttgtgcagc
    17101 gcgtcgtcag tcttcccacg ggcgtcttgt cgtaaaagcg catcgacagc ccttgcagaa
    17161 agtggaagac gtggcgacgc agatccgcca tcgacatcgc gccggcgagc tgcatgctgt
    17221 aaatctgcgc aaacgaaaca aactgcgaca aaacgaccag cccggcgagg attgctccgt
    17281 cgcgcagcag cagatccgcc gctttgtcgc cagcgtgcgc caccactttt cccatcagga
    17341 tcgggcgcag caggttgatc ccagccaccg cgacgagcgc gagcagcgac aaaaccacat
    17401 agcgcgcgtg cggtttgata aacggccaaa gccggctgag aagctgaaag tcgtagctct
    17461 tgtcgatgtt gtcttcgtcg tgaaatgccg ccagcttctg ttcggcgcgg ctcttttcgg
    17521 gggttttttt cttgtcgctc atggccgcac ctcgctgccc ggcagatccg acgcaccgag
    17581 cgcatccagc tcgctggcca tgcgctgttc ttcggcaaac gtggcgtaaa tgccgccttg
    17641 cgccaacagc tgttcgtggg tgccggattc gacaaccttg ccttgatcaa gcaccagaat
    17701 gtgctggcag cgggccgccg ccgcgacgcg gtgggtgatg agcaaaagcg accgctccac
    17761 cgcctgtttt tcgatgctct gcaagatcgc gctttcggtc ttcgcatcga ccgccgacag
    17821 cgggtcgtcc aagataagca gcttcggctg ccacaggagc gcgcgggcca gggcgacgcg
    17881 ctgcttctgc ccgccggaaa gctggacgcc acgttcacca acgatggtat cgagctgttc
    17941 gggcagacct tggacttctt cgagaatctg cgcctcgcgc gcggcttgct ggagccgctg
    18001 ctgcgacgaa ggcgagtccg gttcgtcgag catcagaccg atgttgcgcg ccacggtggt
    18061 cgaaaacaaa aacgcatctt gttgggcgta acccaccgtc gagcgcactt cgcgcagcgg
    18121 cagatcacag acatctttgc catcgagccg cacggtgttt ggcggcgtcg ggataagccg
    18181 cgccaaaagc atcgccagcg tcgatttgcc actgccggtg cgaccgacaa tcgccagcga
    18241 tccgcccgcc ggcagcgtga agctgacgtt tgacagaacc tcgcggtcgc cgtagcggaa
    18301 cgacagacct tgcacttgca gctcgccgcg tggcgggttt ggcaggtggt ctttgccgtc
    18361 gaacagatcg ggctctgcat caatgatttc gcacagccgg ttaaagctta
//
LOCUS       DAORGN010000090        18368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1221_length_18368_cov_6.154917, whole genome shotgun sequence.
ACCESSION   DAORGN010000090 DAORGN010000000
VERSION     DAORGN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1221_length_18368_cov_6.154917"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..649
                     /locus_tag="PKL17_11415"
     CDS             56..649
                     /locus_tag="PKL17_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45387.1"
                     /translation="MGTLVCRIELDKMTGAKVTIENEEGKITQTIHMDGTQILTKVQG
                     EQATSTIVQVADAITVTVKNFTVDAETITLKSSKASQWTSQDTLDIKSTKAMTVKSDA
                     TQAFEAAQDATYKGMNVGIKAQSGLTGEGVNTALKGTASLKGEAPQVELKATASAKME
                     GAMVEIKAQGMLTAESSGMTTLKGSLTSVEGSLVKLG"
     gene            653..2305
                     /locus_tag="PKL17_11420"
     CDS             653..2305
                     /locus_tag="PKL17_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNK45388.1"
                     /translation="MTRDVVEKHLVTELKTLEEFRQRHVQRHPHSRVEREDPDVRRLI
                     EALGFFSVRTRLATLRNLHATWRRLFGTYFGYLLKPLPTRLLVQAQVTPRMVESTLLD
                     AGSELRVTTQNGHVGYFRSSHELRIVPITLESMQLIPLRGGTRLVLNLRSRFPRTDAI
                     GVLRLFIHYIDSYEASLRVHYQLRAHTKRAFAVYEDIVSDETDGPDCGLSFGSLFPEP
                     QQPDTRNPIARVRSFFHFPEEELFVNVDVPRSRKPWSRVALCFDLDDDWPRDPPPTQD
                     MFQLFVVPVENLRKESSEPIHCDGTQEAYAIRHSSPQLGYSLAEETGVFRIDPEGLRP
                     LEPGVLHASSGAMAYEVEERFDDLHNGLFLLVRLPEALLKPMRLVVEGLWHQPGFARD
                     AVGKLKITLPNRNQEGFQFVASSDVRPYSETTLGQDSMALLRLLAMRMKSVLDLRELL
                     QLLIVLGSVKDSPFRDLPQRIRSIKVELAPDSAMRGSGLRHVYRMTFDRFDPDEEALV
                     WTFLSYVRELLDAWNAEATVDLQVDAGGSKLRLPLNPGGAVR"
     gene            2302..2979
                     /locus_tag="PKL17_11425"
     CDS             2302..2979
                     /locus_tag="PKL17_11425"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HNK45389.1"
                     /translation="MMTIELWNVIVSAGTEVRRLLDRALPQTYVAATAQEQTMMLNAG
                     TDVIGLLRTELRRYLDDLKVTFQRILPEKEAGMVLYPLVLHFDEMVTRRLSTAEQTQW
                     PLLQKELFDMNDGGDVFFEFVEERLKKASSPPLVFTVLYYCLSDGFIGKFGQDPAKAA
                     AFKKRLSERIPLPEMPGKTKKKRRKGEDEEAVPLPGKVMHPAVYYLATTGVLVLLFWL
                     VVSMTNL"
     gene            2998..3444
                     /locus_tag="PKL17_11430"
     CDS             2998..3444
                     /locus_tag="PKL17_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45390.1"
                     /translation="MFKLEDGFDDVADALGDVAEEEAEEQAEEFLEDFDADNLLSHWN
                     KKDTTQKQQVIEKRKTRPVHAGTTIRQLLTQLDSQLHNQFGDRATVLQVPFARATAIL
                     RDLFPPSSAPPIVQMDHDPLQKELHDTLNQLEDIMDALLVEAMVRA"
     gene            3451..7335
                     /locus_tag="PKL17_11435"
     CDS             3451..7335
                     /locus_tag="PKL17_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45391.1"
                     /translation="MSLLASALTAQGLSGLASLQAPVPLDFGMLRLPVWVYYALGIVG
                     SLSLVFLIYMAYGYIRRRRDQGDSRPSGGKDAVEATALRDIWNRFHSELPRSARAAVP
                     NYRSFLVLGPASAGKSHLIRARIDWQEQTGQLVPSYTADPMLQIYIGSRALALELSAP
                     ILMSNQDTARDAVSKLFSAAFGSRNPTVVVVLDISAVIRTDPDTLAHQGQSIRAKLNL
                     LAKALRSPIETRLVLSHMEQIEGYSAFAKYLNHERIPLELPLHLLSNREGGLGELLRS
                     YEQYLPHALINVNADQFRGIIKFLHNAPRTLQLLEPLIQPLLQANPAYPTPHLERIYF
                     HAGAKDEQLGNPFHLTRRGEAGRGPRPIISLAWWRGRTHLAVCAACATTAFLILLGLV
                     IRHGRKVHKSENAVEDFAKAVKRASDSLNPSLESAAVRAAEQTSSQLIGALTGADEKW
                     TLYRLLYQDNKEETRQQHIETIRTGYLLPALEKAAKLHQRDKLVYTLAVLYADKENSL
                     GGLVNTQPGEFALTIGIPESVIRDYLAYSEEPWKGSPTVLSTVRSGIRKERGDLVTEE
                     GLWADYFDKVERAVPQQKIGPDELKLLRTGAEPLLATIDAVHSSLSLSRVFRMLSEET
                     PLGDMLAQLSHEDPTLAPPPWLRDQTTPMRAILKLIHDTTADQVRSGRLNLYQVLQLL
                     TDVRSRRDGGLDNHSFELRGHTYSFNSREFLNLLMRSRNQQVVSSLFERRSNGTRPSE
                     TSELERISRRMVKKCLKKCNRMCDAECTIPDMKRYSDLLRKQQSGDSESPTKSAGGHE
                     RHSRRHHLRAGHAEDRVVLSPRLASLVGEKGTLSAAFAGSYNKASFDRDIRPVLTKLD
                     KALEDTPELTTEERDLLARYVANESKNYARRYCEALLAQHRSYVFPGGGVSSTHAELV
                     AAVLPGGSLSSHLKLIADNAQLGDLQSTYLRPLGECIALFQPLVRLMTPGKDGAYPGL
                     KPYAEILGSIVKELDAGKPPIVPEDGKVTPLPDMLSPLGRVALTSLQESPAAVDKQVQ
                     QFLDANGLAGPLSAPFQAPVQRVVRLGQSEIEHTLAEYWQAALRPQVEPMLDKFPFNR
                     SAEGTVEPRELEQLKPGEGAFWVAFRSKYGPVCLERSGEFLPRRFPHGALSFPDGMLA
                     LVNQLGKVSKTLYGKDGSRIPLDLRVRPLPLSAVDGEIPGTLSFLSAGKSVVYGINSA
                     PTPRPLQVAWWQPDAASVGLEYSTADTGRKQTQSLDVGEVNWSFFRLLARGQTSEPRL
                     VTWTLPGDSGRTSRSIKFEFVDDPWAPLKIRR"
     gene            7343..7807
                     /locus_tag="PKL17_11440"
     CDS             7343..7807
                     /locus_tag="PKL17_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45392.1"
                     /translation="MQRLLIDCKRLHHLALGLFFSTLFGCLPGYLRLDIVAPAGLNQG
                     TPVHFVVREVEVNQYRTESYDSVAMLVPNRDASILRSKVLYAKDKGLTERMWIKSAGK
                     KPIAVYFLFTQPKGPWKMLFEPPIPYSATVELDPSSIKPKAQGDLSGSERPF"
     gene            7899..8285
                     /locus_tag="PKL17_11445"
     CDS             7899..8285
                     /locus_tag="PKL17_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HNK45393.1"
                     /translation="MGVQCCMGAQMMCSFGAAPSTLVVLPANKVLATTPAANIMDNKP
                     IVNIPPFGMCMSLANPTVASATAAALGVLTPMPCIPVTTAPWVVGSPTILLGNMPTLD
                     NNSKLMCNWGGVIQIVSPGQFKVMTK"
     gene            complement(8293..9735)
                     /locus_tag="PKL17_11450"
     CDS             complement(8293..9735)
                     /locus_tag="PKL17_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45394.1"
                     /translation="MNTKIDRHVRPTYRKPLFATVVSGILFGALLHGCNGSQVGGDNL
                     DAAGPGGGGTQDMAGGGGTGGDMSGQPGYCAGSGPPIVVGDGSGSVSRCTGQVAAAAF
                     RYGLCLCQGLSSGAPIAIDAFDSTQPGATTFGNGGSAGMNSNVNVSNKFSVGGVLQVS
                     GALTLNELTVGSDLLLGDTLSGNGNVTVAKNAQIGGDVKCNGLLKIGGTLTYPTTRTL
                     SATSQAIGQIARAAVTVAPPCDCSPSSIFDIAGYVTARATSNDNASVPLDPARLTNFA
                     GDQTIDLPCGRFYLNRIGGQGKVTLNITGRTALFVGGDVNLTDTFTVNLGPSGELDLF
                     INGGLTSSAPLNFGNKSAPARVRLYMGGARNINLAAGSVLGGNVYAPNSALVTSGPLE
                     VFGALFVGSFNPSAQVQIHHDIAIIKASQECTPSGSGGGGGSTMCTKCQDCGGQACKS
                     GSCGSCTQNSDCCSPLVCDLRNNTCAVLAG"
     gene            complement(9815..10627)
                     /locus_tag="PKL17_11455"
     CDS             complement(9815..10627)
                     /locus_tag="PKL17_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45395.1"
                     /translation="MQPPNSPLKPTSDAPFASLRAGGADCPSDLMLDRLHSEEIQGKP
                     KAKLEAHLAVCTSCAQRLDVRRAGFSAFADVDERPLLAGVRRKLAESESQTRRGFSWA
                     KLLALVTSATAVSAVAAVVLMTGKPTTLPAPDGVREKGSAALHVYRKVGERAVEALSG
                     DSFAPGDTLRFVVDLPAPSRVSVLGVEASGALYVAWPQDDKAAVAREAGKAQALEGAV
                     VLDEKLGRETLYLVTCPRDGENPATHCKVTAKDQSLACPSACQASPFVLHKK"
     gene            complement(10706..11197)
                     /locus_tag="PKL17_11460"
     CDS             complement(10706..11197)
                     /locus_tag="PKL17_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNK45396.1"
                     /translation="MPESAAKDVGALFRLHGPMVFRRALRLLGRREDAEEATQEVFVR
                     AMRGLHSFDGRSSMSTWLYQITTNYCLNQIRDRGRRRDLFAQNVAADGDEATSAQAAE
                     LVLLRRLLVEADEQQAKAAVYVYLDGMSHEEAAEVLGVSKRTVGNLIERFLGWAKKRT
                     AGP"
     gene            complement(11284..11652)
                     /locus_tag="PKL17_11465"
     CDS             complement(11284..11652)
                     /locus_tag="PKL17_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45397.1"
                     /translation="MWTKTLKVMTLFVGLSTMVACGGGNARCDMREKTPQCTDWRGSL
                     NPTWVTQEALCATLGGTGVGGVFQKGETCPTDGMWGGCQATFADGSKQTNWYYMGDKY
                     KTIESAQSNCDKSMTWLSPQ"
     gene            complement(11731..12522)
                     /locus_tag="PKL17_11470"
     CDS             complement(11731..12522)
                     /locus_tag="PKL17_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK45398.1"
                     /translation="MSGISVDSVPAPAFGLSDELPPLCTAWHKHARHQILYAVAGVMH
                     LDVDRASFLLPPRRAAFLSAGTLHRVRCDRPVSLRTVYVTPDLIGPVAWDCLVFTVSP
                     LLREMMLSAMRWDHKTDPSLPLLRPYFVSLSLLVLELCENAKLPFRLPVPSSDELRRV
                     TQFVRDNLGRPIGVCDLAKAAALSERTLRRRFQSDLGMSPHDYLHAARILAAMERLSD
                     PQQSITMIGLSVGFESSSAFSHAFASFVGETPRDYRRRALATLPL"
     gene            12616..14682
                     /locus_tag="PKL17_11475"
     CDS             12616..14682
                     /locus_tag="PKL17_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK45399.1"
                     /translation="MSLFQQVRLRLASLFLVASVSAVGCGDYSVYDGKISDPWDPGPR
                     KEGSGYGSGSGSGMPPPPPPMCDVSDRRCDFEFVYKGMGGATTKGDEKVVELRGDYRT
                     NGWNMGEALSFDGKVWRLKTTVPWVTKYLYKFRIVDATGKESWIADPENPNKESDGFG
                     GFNSVGSGVTCATYKCEAPPPKCEATAGSFDWRDAVLYFVFVDRFFDGNPANNIPYSG
                     GGGVDPSANWKGGDWAGLLQKLKDGYFESLGVNALWLTVPMDNTDAIGLGDDGRNYTA
                     YHGYWPRDLSKPERRFGTESELKAVVAEAHKRGIKIVIDYAMNHVHKDSPVYAANKSW
                     FNPLDLGGGRQCICGSADCPWEGSKATVCWFRDYLPDFDFTNTAARTFSVDNALSWMK
                     NYEIDGFRLDAVKHIEQVWLTDLRDRITKEVEGVTKQHIYLVGETYTGDRNLIKSFVD
                     PCKKLDGQFDFPLRSELGSKVLLRQGKMQDLVNFMDSNVGFYGTGLNSTFIGNHDVPR
                     SIHLAQNTPLWNDIWAGGKDKNFDPNRPGTVTESEAYERLGLSMAILMTNRGVPLIYY
                     GDEIGLAGAGDPDNRRMMDWNAGGYNSGQKWLLDRMKKLGAIRKAHSSLRRGTRTTLS
                     QSDDTWAYSMVDGTDTVYVAVNRSDSARSVSGLPAMALTDQVSGASTSGPTITVPARG
                     FVILAK"
     gene            14734..16233
                     /gene="pyk"
                     /locus_tag="PKL17_11480"
     CDS             14734..16233
                     /gene="pyk"
                     /locus_tag="PKL17_11480"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNK45400.1"
                     /translation="MSVVSTSPAASLKKHPLASPHRRAKIVCTIGPASRTPEVIGHLI
                     DEGMNVARLNFSHGSADDHRRTVEAVRAEAHKRGKAVAILQDLQGPKIRVGKIKTGTV
                     LEAGTEFILTTEEILGDANRASVTYEGLPRDAQPGNVLLLDDGLLQVEVLSIEGNEVV
                     TRVLVGGPLSNNKGINLPGVKVSAPSMSDKDHADLIVGQKLGVDFLALSFVRSAQDIH
                     DARAAILAARKDIPESQSRPPTPIIAKIEKPEAVDRLEEIIEAADGIMVARGDLGVEL
                     GPEKVPLVQKAAIQLANAHAKLVITATQMLESMTQNARPTRAEASDVANAVMDASDAL
                     MLSAESASGKYPLLAVRTMDRIIREVEQSERYFTIMRQLEWPMIRVTSNAVAHAAVVA
                     SRLVEAPVIVALSNSGGAARLLSEYRSEALVVGMTSSVDIYNQLAAYWGILPMMAPQA
                     EDETTMIRAVLSLVRDRGLAKPGQHVILTMAIPFRSGLPTNTMQIHRVE"
     gene            16309..17748
                     /gene="pcnB"
                     /locus_tag="PKL17_11485"
     CDS             16309..17748
                     /gene="pcnB"
                     /locus_tag="PKL17_11485"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNK45401.1"
                     /translation="MLAPTIPIDQIAPEAQKVIRHLVRSGHQAYLVGGCVRDLLLGRT
                     PKDFDVATDATPSELRELFRNCRIIGRRFRLAHIIFGPKIIETATFRANPREDEDGQQ
                     ATDELYIHRDNVFGTAEQDARRRDFTINGLFYDLPAEKVIDYVDGLRDLESRFVRTIG
                     DPNVRFREDPVRMLRAVKFAARLGFDIEDTTYRALLSHGKEIEKSPVPRVLEEIFRLL
                     RGGAAMESMVLLRECGMLDVLLPEVTPQLAAHPGLLDPYLHAIDRSVEKGDVPVNAVL
                     VLALFMPKLLPLFEAGEKRQGLSVDALSDVLVPLCSRLKVPRRDVEQAKNLLIVQRRI
                     AQARTRGGKLPPSVLHREELRPALGLHALVYRCQRQAEGASDAAIDSEIAAWPLPGPF
                     FLGAGDSARSEFAPEREFRRDDRRRRSRRPEIDDLAPRPRLPEVSSPEAGVTNERAAS
                     SGDDEPLLDDEEAALAAFVAAMRSGRDDD"
     gene            17797..>18368
                     /locus_tag="PKL17_11490"
     CDS             17797..>18368
                     /locus_tag="PKL17_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNK45402.1"
                     /translation="MASASTQTPKPHLATFADLLRIPEDRRRHEILDGEIIEKALPRW
                     EHGVGQTRAGELLRRFNRKPNGPTRPGGWWILTEPTIQLSRHELVQPDLAGWRRDRVH
                     DEPPSYPITLRPDWVCEIMSDGDARRRDGLQKRRIYADHDVPFYWLIDTERETLTVLS
                     LTEECYVEQLTACRTDRVRAAPFDALELQIG"
BASE COUNT         3922 a         5691 c         5237 g         3518 t
ORIGIN      
        1 atccaggcca aggagggcta cctcctcatc gaagtgaagg aggagaagta gtcccatggg
       61 cacgctggtt tgtcgaatcg aactcgacaa aatgaccggt gcgaaggtca cgatcgaaaa
      121 cgaggaaggc aagatcacgc agaccattca catggatggg acgcaaatct tgaccaaggt
      181 gcaaggcgaa caagcgacga gcaccatcgt tcaagtggcc gacgccatca ccgttacggt
      241 gaagaacttc acggtcgatg cagagaccat cacgctcaag tcgagcaaag cctcgcagtg
      301 gaccagtcag gacacgctcg atatcaagag cacaaaagcg atgacggtca aaagtgatgc
      361 gacacaggct ttcgaggccg cgcaagatgc gacgtacaaa ggaatgaacg tcggcatcaa
      421 ggcccagagc ggactgaccg gagaaggggt caatacggcg ctcaagggaa ccgcatcgct
      481 caagggagaa gcaccccaag tcgagctaaa ggcgacggcg agcgccaaga tggaaggggc
      541 gatggtcgaa atcaaagcac agggaatgct gaccgcagag tcttccggaa tgaccacgct
      601 gaaagggtct ctgaccagcg tggaaggatc gcttgtgaag ctgggctaag ccatgacacg
      661 cgatgtagtg gaaaagcact tggtgacgga gctaaagacg ctggaagagt tccgccagcg
      721 ccatgtccag cgtcatccgc acagccgcgt cgagcgcgaa gatcccgatg ttcgacggct
      781 gatcgaagca ctgggattct tttcggtgcg aacacggctg gccacgctgc gcaatctgca
      841 cgcaacctgg cgccgtctgt ttgggacgta ctttggctat ctgttaaagc cgctgcccac
      901 gcggctgctc gtccaggcgc aggtgacgcc acggatggtc gaaagcacac tgcttgatgc
      961 aggcagcgag ctgcgcgtca ccacgcagaa cggacacgtt gggtatttcc gatccagcca
     1021 cgagctgcgc attgtcccga ttacgctgga atcgatgcag ctaattccgc tgcgcggagg
     1081 cacgcgactt gtactaaacc tgcgctcgcg ctttccgcgc acggacgcga tcggtgtcct
     1141 gcggcttttc atccattaca ttgattccta tgaagcgtcg ctgcgcgttc actatcagtt
     1201 gagagcgcac acaaagcgcg cctttgcagt ctatgaagac atcgtcagcg acgaaacaga
     1261 tgggccagac tgcggactca gctttggttc tctgtttccg gaaccgcagc agccagacac
     1321 gcgcaatccg attgccagag tgcgttcctt tttccatttt ccggaagaag agctgtttgt
     1381 aaacgtggac gtaccgcgct cacgcaagcc gtggagccgg gtggcactgt gtttcgatct
     1441 cgatgatgac tggccgaggg atccgccgcc gacgcaggac atgtttcagc tttttgtggt
     1501 gccggtggaa aatctccgca aggaaagcag tgaaccgatc cactgcgacg gaacccaaga
     1561 agcgtatgcg attcgccaca gcagtcccca gctgggttat tcccttgcgg aagagaccgg
     1621 cgtgttccgc atcgatccag aaggactccg tcccctggaa cctggcgtgc tgcacgcctc
     1681 aagcggtgcg atggcgtatg aagtcgagga acggttcgac gatttacaca atggactgtt
     1741 ccttcttgtg cggctcccag aagcactcct aaagccgatg cggctggtgg tggaaggtct
     1801 gtggcatcag cccggatttg cccgcgacgc ggttgggaag ttgaaaatca cgcttcccaa
     1861 tcgcaaccaa gaaggattcc agtttgtcgc cagcagcgac gtgagaccgt attccgaaac
     1921 gacactggga caggactcga tggcgctgtt gcggctgttg gcgatgcgca tgaaatcggt
     1981 gctggatctg cgcgagctct tgcagcttct tatcgtgctg ggcagcgtga aggactcacc
     2041 atttcgcgat ctgccacagc gcattcgcag catcaaagta gagctggccc cagacagtgc
     2101 gatgcgcggt tcgggtctgc gccatgtcta tcggatgacg ttcgatcgct tcgatcccga
     2161 cgaagaagcc ctggtgtgga cattcctatc ctatgttcgc gagctgctgg atgcatggaa
     2221 tgccgaagcc actgtggatc tccaagtcga tgccggtggt agcaagctgc gcttgccgct
     2281 aaatccaggg ggtgcggtcc gatgatgacg attgagctgt ggaatgtcat cgtgtcagca
     2341 ggaacggaag tgcgtcgatt gctcgatcgc gcgctgcccc aaacatatgt cgcagcgacg
     2401 gcgcaagagc agaccatgat gctcaatgcc ggcaccgatg tgatcggtct tttgcgcaca
     2461 gagctgcgcc gctatctgga tgatctgaaa gtgacgtttc agcgcatttt gccggaaaag
     2521 gaagccggca tggtgctgta cccactggtg ttgcatttcg atgaaatggt gacgcgccgc
     2581 ctgtcgaccg ccgagcagac ccagtggcca cttctgcaaa aagagctgtt cgacatgaac
     2641 gatggtggcg atgttttttt cgagttcgtg gaagagcgac tcaaaaaagc atccagtccg
     2701 cccctggtgt tcactgttct ttactactgc ttaagcgacg gattcatcgg caagttcgga
     2761 caagacccgg ccaaggccgc agcgttcaaa aagcgcctgt cggaacgcat tccgctgccc
     2821 gaaatgccgg gcaaaacgaa aaagaaaaga cgcaaaggtg aagatgaaga agccgtcccc
     2881 ctgcctggca aagtgatgca tcccgcggtg tactacctgg caaccacagg cgtactggtg
     2941 cttttgtttt ggctggttgt ttcgatgacc aatctctagc cgaaaggaat tgtgaacatg
     3001 ttcaagctag aagatggttt tgatgatgtc gcggatgcgc tgggcgacgt ggcagaagaa
     3061 gaagcagagg aacaagcaga ggagtttctg gaagacttcg atgccgacaa tctgctgtct
     3121 cactggaaca aaaaggacac cacgcaaaag cagcaggtga tcgaaaaacg gaagacgcgc
     3181 ccggttcatg caggcacgac gataaggcag ctacttacgc agcttgacag tcagcttcac
     3241 aatcagtttg gtgatcgtgc gacggtactg caagttccgt ttgcgcgcgc caccgcgatc
     3301 ctgcgcgatc tgtttccacc cagttcggct cccccgattg tacagatgga tcatgatccg
     3361 ctgcaaaagg agctgcacga cacactcaac cagctagagg acatcatgga tgcactcttg
     3421 gttgaagcca tggtgagggc ttaggtggtg atgagtctgc tcgcaagcgc actcactgcg
     3481 cagggacttt cgggacttgc cagtctgcag gccccggtgc cgcttgattt tggcatgctg
     3541 cggcttcctg tctgggtgta ctatgcgctg ggaatcgttg gcagtctgtc gcttgtattc
     3601 ctgatctaca tggcctacgg atacatccgc aggcgacggg atcagggaga ttccagacca
     3661 agtggtggca aagatgcagt ggaagccacc gcgctgcgtg acatctggaa tcgctttcat
     3721 tccgagcttc cccgatcggc gcgcgccgcg gttcctaact accggagttt tttggtactg
     3781 ggtccagcca gcgccgggaa aagtcacctg atccgggcac gcatcgactg gcaggaacag
     3841 accggacagc ttgtaccaag ctacaccgcc gacccgatgc tgcagattta catcggaagt
     3901 cgcgcactgg cactggagct gtctgcgccg attctgatgt cgaatcagga tactgcgcgc
     3961 gatgcggtat cgaagctatt ttccgccgcg tttggcagcc gcaatccgac cgttgtggtg
     4021 gttctcgaca taagtgcggt catccgaacc gatccagata ccctggcaca tcaaggacag
     4081 tcgatccgcg ccaagctgaa cctgctggca aaagcactcc gttcaccgat tgagacgcga
     4141 cttgtgctgt cgcacatgga acagatcgaa ggatactctg cgtttgccaa gtatctgaac
     4201 catgaacgga ttccgcttga gctgccactg catcttttga gcaatcggga aggcggtctg
     4261 ggagagcttc tgcgcagcta cgaacagtat cttccgcatg cgctcatcaa cgtcaacgcc
     4321 gatcagttcc gcggcatcat caagtttttg cacaacgcgc cgcggacact ccagctgttg
     4381 gaaccgctga tccagccgct gttgcaagcg aaccctgcgt atccaacgcc ccacctggaa
     4441 agaatttatt tccatgcggg agccaaagac gaacagcttg ggaatccgtt tcatcttacc
     4501 cgccgcggtg aagcaggacg tggaccacgg cccatcattt cgcttgcttg gtggcgcgga
     4561 cgaacccatc tggcagtctg cgcggcttgt gccacgacgg cgtttctcat tctgctgggt
     4621 cttgtgatcc gccatggtcg caaagttcac aagtcggaaa acgcggtcga agattttgcc
     4681 aaggcggtca aacgcgccag cgattccctc aacccatcgc tcgagtctgc cgcagtccgt
     4741 gccgccgaac agacatcaag ccagctgatt ggcgcgctga ccggtgcaga tgaaaagtgg
     4801 acgctgtatc ggctcctgta tcaagacaac aaagaggaaa cgcgccagca gcacatcgag
     4861 acgattcgca ccggatatct gctgcccgcc ctggaaaaag cggccaagct acatcagcgc
     4921 gacaagctgg tctacacgct tgcggtgctg tatgccgaca aggaaaactc gctgggagga
     4981 ctcgtcaaca cccagccagg agagtttgcg ctgaccatcg gaattcccga aagcgtcatc
     5041 cgcgactacc ttgcatactc cgaagagccg tggaaaggca gcccgaccgt acttagcaca
     5101 gtgcgcagtg ggattcgcaa agagcgcggt gatttggtga cggaagaagg tctgtgggcg
     5161 gactactttg acaaggtgga acgggccgtt ccacagcaaa agataggacc cgatgaactg
     5221 aagcttctgc gcacaggagc ggagccgctg cttgcaacca tcgacgcagt acactcctcg
     5281 ctgagtctgt cgcgggtgtt ccggatgcta tcggaagaaa ccccgctagg tgacatgctg
     5341 gcccagctat cgcatgaaga tccaacgctg gcgccgccac cttggctgcg ggatcagacc
     5401 accccaatgc gggcgattct gaagctgatt cacgacacca cagcggatca ggtacgcagc
     5461 ggaagactca acttgtatca agttcttcag ctgctaacag atgtccgttc tcgtcgagac
     5521 ggcggtctgg acaatcactc ctttgagctg cgaggccata cctattcctt caactcgcgt
     5581 gagtttctga atctgctgat gcgcagccgc aaccagcagg ttgtaagctc tctgttcgag
     5641 cgtcgttcaa acggaacccg gcccagcgag acatcggaac ttgaacgaat ttcgcgccgg
     5701 atggtgaaaa agtgtctgaa aaaatgcaac cggatgtgcg atgccgaatg cacgattccg
     5761 gacatgaagc gctattctga tctgctgcgc aaacagcaaa gcggcgatag tgaaagtccc
     5821 accaagtcag caggaggaca cgagcgtcac agtcgacggc atcatctgcg cgcaggacat
     5881 gccgaagatc gggtggtttt gtctccgcga ctcgcttcac tggttggtga aaagggaaca
     5941 cttagcgcag cgtttgctgg ttcctataac aaagccagct ttgatcgtga catccgcccg
     6001 gtgctgacca agctcgacaa agcgctggaa gatacgccgg aacttacgac agaggaacgc
     6061 gatctgctcg ctcgctatgt cgcaaacgag tcaaaaaact atgcacgcag gtactgcgaa
     6121 gcactccttg cacagcaccg aagctatgta tttcccggcg gtggcgtaag ctcaacccac
     6181 gcggaactgg tcgcagctgt gctgcccggt ggatcgcttt ccagtcacct gaagttgatt
     6241 gcagacaatg cccagcttgg ggatttgcag tcgacctatc tgcgtccact tggcgaatgc
     6301 attgcgctgt ttcagccgct tgtgcgactc atgacaccag ggaaagacgg cgcttatccg
     6361 gggctaaaac cctacgcgga aatcttggga tcaatcgtca aggaactgga tgctggcaag
     6421 cccccaatcg ttccggaaga tggcaaagtc acaccgcttc ccgacatgct gtcgccgctg
     6481 ggtcgtgtcg cgctcacgtc gctgcaagag agtccggctg cggttgacaa acaggttcag
     6541 cagtttctgg atgcaaacgg tctggccggt cccctgtcgg ccccgtttca ggcccctgtg
     6601 cagcgcgtcg tgcgtttggg acaatcggaa atcgaacata cattggccga gtattggcag
     6661 gctgcgctgc gtccacaagt cgagcccatg ctcgacaagt ttccgttcaa tcgcagcgcg
     6721 gaaggaacag tggaaccgcg cgagctggag cagctaaaac ccggtgaagg tgcgttctgg
     6781 gtcgcgttcc gcagcaagta tggacctgtc tgtttggaac gcagtggtga atttctgccg
     6841 cgccgctttc cgcatggcgc gctcagcttt cctgatggaa tgctcgcgct tgtgaaccag
     6901 cttgggaagg tgtcaaagac gctgtatggg aaagatggat cgcgcattcc gctcgatctg
     6961 cgggttcgtc cgctgccgct cagtgccgtc gatggtgaaa tccccggaac gctgtccttt
     7021 ctttccgcgg gaaaatccgt ggtctacggc atcaattccg cgccaacgcc acgtccgctg
     7081 caagttgcgt ggtggcaacc ggatgcagcc tctgtgggtc tggaatacag caccgcagac
     7141 accggacgca aacagacgca gtcgctcgac gtgggtgagg tcaactggag ctttttccgc
     7201 ttgcttgcgc gcggacagac cagcgagcca cggcttgtca cctggacgct tccgggcgac
     7261 agcggacgca catcacgcag catcaagttt gagtttgttg atgatccgtg ggcaccgcta
     7321 aaaattcggc gataggtttt ccatgcagcg acttttgatt gattgcaaac gactgcacca
     7381 cctggcgctg ggcctatttt tttcgacgct gtttggctgt cttccgggct atctgcggct
     7441 cgacattgtg gcgccggcgg ggctcaatca ggggactccg gttcactttg tggtacgcga
     7501 agtggaagtg aatcagtacc gaacagagtc ctatgattcc gtcgccatgc tggttcccaa
     7561 tcgcgacgcg agcatccttc ggtccaaagt cctgtatgcc aaagacaaag gcctgaccga
     7621 acggatgtgg attaagagcg ccggcaagaa acccatcgcc gtgtactttc tgttcaccca
     7681 gcccaaaggt ccgtggaaga tgctgtttga acccccgatt ccgtacagtg ccaccgtcga
     7741 actggacccg agcagcatca agccgaaagc ccagggtgat ctgtccggtt ccgagcgtcc
     7801 gttttagcgt catgtcaaaa tcatcaggag tccgtgaccc aaacggaatc acccggtttg
     7861 gtccagtgtg actgtcctat tttgtttggg agttcatcat gggagttcag tgttgtatgg
     7921 gtgcgcagat gatgtgtagc ttcggagccg cgccgtcgac gctcgtggtt ctgccggcaa
     7981 acaaagtgct cgcgaccaca ccggctgcca acatcatgga caacaaaccg attgtgaaca
     8041 ttccaccgtt cggaatgtgt atgtcgctgg ccaatcccac cgttgcgtct gccactgcgg
     8101 cagcgctcgg agtcctgacg ccgatgccgt gcattcctgt gacgacagcc ccgtgggtcg
     8161 ttggctcacc gacgattttg ctcggcaaca tgccaacgct cgacaacaat tccaagctga
     8221 tgtgcaactg gggcggcgtc atccagatcg tctcccctgg tcagttcaaa gtgatgacca
     8281 aatagcgcgg actcacccgg ccaacacggc gcaggtgttg ttgcgaaggt cgcacacaag
     8341 cggcgagcag cagtcggagt tttgcgtgca gcttccacag cttccggact tgcaagcttg
     8401 gccgccgcag tcttggcact tggtacacat cgtcgatccg cccccgccac ccgatccgct
     8461 cggcgtgcac tcctgtgatg ctttgatgat cgcgatgtcg tggtgaatct gcacctgcgc
     8521 gctcgggttg aagctgccga caaacagcgc gccgaatact tccagcggtc ctgatgtgac
     8581 cagcgcagag tttggcgcat agacattgcc gccaagcacg ctccccgccg cgaggtttat
     8641 gttgcgagcg ccccccatgt aaagccggac ccgcgctggt gcacttttgt taccgaagtt
     8701 gagcggagcc gagctggtca agccaccgtt gatgaacaga tccagctcgc cgcttgggcc
     8761 caggttcacg gtgaacgtgt cggtcaagtt gacgtctcca ccgacgaaca gagccgtccg
     8821 tccggtgatg ttgagcgtca ctttgccctg gcccccgatc cgattgaggt aaaagcgtcc
     8881 gcacggcaag tcgatcgtct ggtcgcccgc aaagttggtc agccgcgccg gatcaagcgg
     8941 caccgaagca ttgtcattgc tcgtcgcccg cgccgtgacg tacccggcaa tgtcaaaaat
     9001 gctcgatggt gagcagtcac acggcggtgc caccgtgacc gcagcgcgcg caatctgacc
     9061 aatcgcctgc gaggtcgcgc tgagcgttct ggtcgtcgga tacgtcagtg tgccgccgat
     9121 ttttaaaaga ccgttgcact tgacgtcgcc gccaatctgc gcgttttttg cgacggtgac
     9181 gttgccattg cccgacagcg tatcgcccag caaaaggtcg cttccgacgg tcagttcgtt
     9241 cagcgtcaga gcgccactga cttgcagtac accaccaacg gaaaatttgt tgctgacgtt
     9301 gacattcgag ttcatgcccg ccgatccgcc gttgccaaac gtcgtcgctc ccggctgcgt
     9361 actgtcaaat gcatcaatcg caatcggtgc gcccgatgaa agcccttggc acaggcagag
     9421 cccatagcga aacgccgccg ccgcgacttg tccggtacac cttgaaaccg aaccgctgcc
     9481 gtcgcccacc acaatcggtg gcccgctgcc cgcgcagtaa ccgggctggc cggacatatc
     9541 gccacccgtt ccgccgccgc cggccatgtc ttgtgtgccg ccgccgccgg gacctgccgc
     9601 atccaggttg tcgccgccga cttgactgcc gttacagcca tgcaaaagcg cgccaaacag
     9661 gattccggaa acgacggtgg caaacagtgg cttgcgatag gtagggcgaa cgtggcgatc
     9721 gattttggtg ttcatacaga aattgtagct tctgtagaaa gtgggcaaat acgtgttctc
     9781 gacgagaatt agtttcccaa gtgcgcaagc gaagttactt cttgtgcagc acaaatgggc
     9841 tcgcttggca ggctgaagga caggcaagcg actggtcttt tgcggtcact ttgcagtgcg
     9901 tcgccgggtt ttccccatcg cgcggacacg tcaccagata cagcgtctcg cgtcccagtt
     9961 tttcgtcgag aaccaccgca ccttccagcg cctgtgcttt gcccgcctca cgcgctacgg
    10021 ctgccttgtc atcttgcggc caggccacgt acagcgcacc gctcgcttcc accccgagca
    10081 cgctgacccg actgggcgca ggcaagtcga cgacaaagcg cagcgtgtca ccgggcgcaa
    10141 aggaatcgcc gctcagcgcc tcgacggcac gctcgccgac tttgcgatag acatgcagcg
    10201 ctgcactgcc cttttcgcgc acgccatctg gcgcgggcaa cgtcgtcggc ttgcccgtca
    10261 taagcacaac cgctgccact gctgaaaccg ccgtggccga tgtcacaagc gccaacagtt
    10321 tcgcccagga aaaaccccgg cgcgtctgcg attccgattc cgccagcttg cgacgcaccc
    10381 cggccagcag gggcctttca tcgacatccg caaacgccga aaatccggca cgccgcacgt
    10441 caagacgctg agcacacgac gtacaaaccg ccagatgcgc ttccagcttg gctttgggct
    10501 tgccttggat ttcttcgctg tgcaagcgat ccaacatcag atcggacgga cagtccgccc
    10561 cacctgcgcg cagcgatgca aacggcgcat cggatgttgg tttgagcggt gagtttggcg
    10621 gctgcataaa accctcttgg cgcgatctgt ttcgtccctg ctgtctatga cccgcaggcg
    10681 agcggacttg ggcaagcaaa ccgcatcacg gccccgctgt ccgcttcttg gcccagccca
    10741 ggaaccgttc gatcaagttt ccgacggtgc gcttgctcac cccgagcact tccgccgctt
    10801 cttcatggct catgccatcg agatatacgt acaccgcggc cttggcttgc tgttcatcgg
    10861 cttcgacgag caagcggcgc aagagcacaa gctctgctgc ctgggccgaa gtcgcttcat
    10921 cgccatccgc agcgacattc tgtgcaaaca gatcacgtcg ccgtcctcga tcgcgaatct
    10981 ggttcaaaca gtagtttgtc gtgatctggt aaagccacgt cgacatgctg ctgcgtccgt
    11041 cgaagctgtg cagaccacgc atcgcccgca cgaacacttc ttgggtcgct tcttcggcat
    11101 cttcacgccg gcccaaaagc cgcagagcac gacggaagac catcggacca tgcagacgga
    11161 acagcgcccc tacgtctttg gccgcagatt caggcacagt agcagtcagc gtcacttgct
    11221 gtgccaccga ctgtcaagtc cgcgaaagcg cttttgtttg tgggaagtgt gggcgaagca
    11281 aaactactgc ggcgacagcc acgtcatcga cttgtcgcag ttcgactgcg cagactcgat
    11341 ggttttgtat ttgtcaccca tgtagtacca gttggtctgc ttgctgccgt cggcgaatgt
    11401 cgcttggcag ccgccccaca tgccgtccgt cggacacgtt tcgcccttct ggaacacgcc
    11461 gccgaccccg gttccaccga gcgtcgcgca cagcgcttct tgcgtcaccc acgtcggatt
    11521 cagcgagccg cgccagtcgg tacactgcgg tgttttttcc cgcatgtcac agcgcgcatt
    11581 gccgccccca cacgccacca tcgtcgacaa accaacaaaa agagtcatca ctttcagcgt
    11641 ctttgtccac attgcacagt cactcctttg ccaatccccc acgggccccc gtcacccaat
    11701 gccggaccag gcccccgagg caaccagaca tcatagcggc aacgtcgcca aagcgcgcct
    11761 gcggtaatcc cgcggtgttt caccgacgaa cgatgcaaag gcgtggctaa acgcactgga
    11821 gctttcaaaa ccgaccgaaa ggccaatcat cgtgatggac tgctgcggat cggacagtcg
    11881 ttccatcgcg gccaaaatcc gcgctgcgtg cagatagtcg tgcgggctca tccccagatc
    11941 gctttggaac cgcctgcgca gcgtgcgttc ggacagcgcc gccgctttcg ccaggtcaca
    12001 aaccccaatc ggacggccca agttgtcgcg cacaaactgt gtcacccgtc gcagctcgtc
    12061 cgatgacgga accggcaaac ggaacggcag cttggcgttt tcacacagct ccagcacaag
    12121 caagctcagg ctgacaaaat acggacgcag cagcggcagc gacggatcgg tcttgtgatc
    12181 ccagcgcatc gccgacagca tcatttctcg caaaagcggc gacaccgtga acaccaggca
    12241 gtcccacgca accggcccga tcagatccgg cgtcacatag accgtgcgca gcgacacagg
    12301 ccgatcacag cgcacccgat gcagcgttcc cgcggacaga aatgcagccc gccgcggcgg
    12361 cagcaaaaag ctcgcgcggt cgacgtccag gtgcatcacc cccgccaccg cgtacaggat
    12421 ttggtgacgc gcgtgcttgt gccaggccgt gcagagcggc ggaagctcat cggacagacc
    12481 gaacgccggg gccgggactg aatcaacaga aatgccagac acgagcgcag catagtcgcc
    12541 cgcgcacgtt caatttgccc ttttgtggcg gcgccgtttc gttatcgtag cgtcccattc
    12601 cgcaaggagg tgcgcatgtc gctttttcag caggtccggc tccggcttgc ctctctgttt
    12661 ctcgtcgcca gcgtgtcagc agtcggctgc ggcgactaca gcgtttacga cggcaaaatc
    12721 tctgatccgt gggaccccgg cccgcgcaaa gaaggatcgg gatacggatc gggatcaggg
    12781 tcgggcatgc cgccgccacc accgccgatg tgcgatgtca gcgatcgccg ctgtgacttt
    12841 gaatttgtct acaaaggcat gggcggcgcg acgaccaaag gcgacgaaaa agtcgtcgag
    12901 ctgcgcggcg attaccgaac caatggctgg aacatgggcg aagcgctgtc gttcgacggc
    12961 aaagtctggc ggctcaagac caccgtcccg tgggtcacaa agtacctgta caagttccgc
    13021 atcgtcgacg ccactggcaa ggaaagctgg atcgccgacc ccgaaaaccc aaacaaggaa
    13081 tcggacggtt ttggcggctt taactccgtc ggcagcggcg tcacttgcgc aacctacaaa
    13141 tgcgaagcac cgccgcccaa atgtgaagcc accgcgggca gctttgactg gcgcgacgca
    13201 gtgctctact tcgttttcgt cgatcgattc ttcgacggaa atcccgccaa caacatcccg
    13261 tacagcggtg gtggtggcgt cgatccgtcg gccaactgga aaggcggcga ctgggcagga
    13321 ctgctccaga agctcaaaga cggctacttc gaatcgctcg gcgtcaacgc gctgtggctc
    13381 accgtcccga tggacaacac cgatgcgata ggtctgggcg acgacggacg caactacacc
    13441 gcgtaccacg gctactggcc gcgcgatctg tccaagcccg aacgccgctt cggcaccgaa
    13501 tccgagctaa aagccgtcgt cgcggaagcc cacaaacgcg gcatcaaaat cgtcatcgac
    13561 tacgcgatga accacgtcca caaagactcg ccggtgtacg ccgccaacaa gagctggttt
    13621 aacccgctcg acctaggagg cggacgccag tgcatctgcg gatctgccga ctgtccgtgg
    13681 gaaggcagca aagccaccgt ctgctggttc cgcgactacc tgcccgactt tgactttacg
    13741 aacaccgcgg cgcgcacgtt ttccgtcgac aatgcccttt cgtggatgaa aaactacgaa
    13801 atagacggct tccgtctcga cgccgtaaag cacatcgaac aggtgtggct gaccgacctg
    13861 cgcgaccgca tcaccaaaga agtcgaaggc gtcaccaaac agcacatcta ccttgtcggt
    13921 gagacgtaca caggcgatcg caacctgatc aaatcgtttg tcgatccgtg caaaaagctc
    13981 gacggccagt tcgacttccc gctgcgatca gagcttggca gcaaggtgct cttgcgccag
    14041 ggcaagatgc aggatctggt gaactttatg gactcgaacg tgggctttta cggcactggg
    14101 ctcaactcga cgttcatcgg caaccacgat gtgccacgct cgattcacct ggcgcaaaac
    14161 acaccgctgt ggaacgacat ctgggccggc ggcaaagaca aaaactttga tccaaaccgc
    14221 cccggcaccg tcaccgaaag cgaagcctat gaacggcttg ggctgtcgat ggcgattctg
    14281 atgacaaacc gcggcgtccc gctcatctac tacggcgacg agatcggtct ggccggcgcg
    14341 ggcgaccccg acaaccgccg catgatggac tggaacgccg gcggctacaa cagcggccaa
    14401 aagtggctgc tcgaccggat gaaaaagctc ggcgcgattc gcaaagccca cagcagcctg
    14461 cgccgcggca cccgcaccac cctgtcgcag tccgacgaca cctgggcgta cagcatggtc
    14521 gacggaaccg ataccgtcta cgtcgcggtc aatcgttcgg attcggcgag gagcgtgtcg
    14581 ggtctgccgg ccatggcgct taccgatcag gtcagcggcg caagcacaag cggcccgacc
    14641 atcaccgtcc ccgcccgtgg cttcgtcatc ctcgccaaat agcctcgaaa agcccccacg
    14701 cggtgggcgg gtcgcgatat aaacatctct acgatgtctg tggtctccac ctctcccgct
    14761 gcttccttaa aaaagcatcc tctggcgagc ccgcaccgtc gggccaagat tgtctgcacc
    14821 attggcccgg ccagtcgcac cccggaagtg attggccacc tcatcgacga aggcatgaat
    14881 gtcgcgcggc tgaacttttc gcacggcagc gccgatgatc accgccgcac cgtcgaagcc
    14941 gtccgcgccg aagcgcacaa gcgcggcaaa gccgtggcga ttttgcaaga cctgcaaggc
    15001 cccaaaatcc gcgtcggcaa gatcaagacc ggcaccgtgc ttgaagccgg cacagagttc
    15061 atcctcacca ccgaggagat actgggcgac gcaaaccgcg ccagcgtaac ctacgaaggt
    15121 ctgccgcgcg atgcccagcc cggcaatgtg ctcctgctcg acgacggact tttgcaagtc
    15181 gaagtgctgt cgattgaagg aaacgaagtc gtcacccgcg tgctcgtcgg gggtccactg
    15241 tccaacaaca aaggcatcaa cctgcccggc gtcaaagtgt ccgcgccgtc gatgtccgac
    15301 aaagaccatg ccgacctgat cgtcggacaa aagctcggtg tcgattttct ggcgctgtcg
    15361 tttgtccgtt cagcccagga catccacgac gcccgcgccg ccattttggc cgcccgcaaa
    15421 gacattcccg aaagccaaag ccgtccgccg acgccgatca tcgccaagat cgaaaaaccc
    15481 gaagccgtcg accgactcga ggaaatcatc gaagccgccg acggcatcat ggtcgcgcgt
    15541 ggcgatctgg gcgtggagct cggccccgaa aaagtgccgc tcgtgcagaa ggccgcgatt
    15601 cagcttgcca acgcacacgc caagctggtc atcaccgcga cgcaaatgct cgaatcgatg
    15661 acccagaatg cccgcccgac ccgcgccgaa gccagcgacg tcgccaacgc cgtcatggat
    15721 gcatcggatg cgctgatgct gtcggctgaa tcggcttcgg gaaaatatcc gctgctcgcc
    15781 gtccgcacca tggaccgaat catccgcgaa gtcgagcaga gcgagcgcta ctttacgatc
    15841 atgcgccagc tcgagtggcc gatgattcgc gtcacttcaa acgccgtcgc ccacgccgcc
    15901 gtcgttgcct cgcgtctcgt cgaagccccg gtcatcgtcg cgctgtccaa ttccggcggc
    15961 gccgcccgcc ttttgtccga ataccgctct gaagccctgg tcgtgggcat gacttccagc
    16021 gtcgatatct acaaccagct tgccgcctac tggggcatcc tgccgatgat ggcgccgcag
    16081 gccgaagacg aaacgacgat gattcgcgcc gtgctgagcc tggtccgcga ccgcggcctc
    16141 gccaaacccg gccagcacgt catcctcacc atggccatcc ccttccgatc tggtcttccc
    16201 accaacacca tgcaaatcca ccgcgtcgaa tagtcccgcg atcccctgct tcttttcgag
    16261 cgtagaaatt gcgatgtggg cccagattgt gataggcccc atgcaggcat gcttgcgccg
    16321 accattccga tagatcaaat cgcccccgag gctcaaaagg tgatccgtca ccttgtccgc
    16381 agcggacatc aggcctatct cgtcggtggc tgcgtgcgcg atctgctgct cggtcgcaca
    16441 cccaaagact tcgacgtcgc caccgatgcc accccgagcg agctgcgcga gctgtttcgc
    16501 aactgccgca tcatcggtcg ccgcttccgt ctcgcccaca tcatttttgg gcccaagatc
    16561 atcgaaacgg cgacctttcg cgccaatcct cgcgaagatg aagacggcca gcaggcgacc
    16621 gacgaactgt acatccaccg cgacaacgtc tttggcaccg ccgagcaaga cgcccgccga
    16681 cgcgacttta cgataaacgg tcttttttac gacctgcccg ccgaaaaagt catcgactac
    16741 gtcgacggcc tgcgcgacct cgaaagccgc tttgtccgca ccatcggcga tcccaacgtc
    16801 cgcttccgtg aagacccggt gcggatgctg cgcgccgtca agtttgccgc ccggctcggc
    16861 ttcgacatcg aagacaccac ctatcgcgcc ctgctcagcc acggcaaaga gatcgaaaaa
    16921 agcccggttc cgcgcgtgct cgaagaaatc ttccggcttc tgcgcggtgg agcggcgatg
    16981 gaatccatgg tgctgctgcg ggaatgcggc atgctcgacg tgctgctgcc cgaggtcacg
    17041 ccgcagcttg ccgcgcaccc cggcctgctc gatccgtacc tgcacgccat cgatcgcagc
    17101 gtcgaaaaag gcgatgtccc ggtcaatgcc gtgctcgtcc ttgcgctgtt tatgccaaag
    17161 cttctgccac tgtttgaagc cggcgaaaag cggcaaggtc tgtctgtcga tgcgctcagc
    17221 gacgtgcttg tgcccctgtg cagccggctg aaagtcccgc gtcgcgatgt cgagcaagcc
    17281 aaaaacctgc tgattgtgca aagacgcatc gcccaggcgc gcacccgtgg gggcaagctg
    17341 ccgccgtccg ttttgcaccg cgaggagctg cgtccggcgc tgggtctaca cgcccttgtc
    17401 tatcgctgtc agcgtcaagc cgagggcgca agcgatgcag ccatcgacag cgaaatcgcc
    17461 gcctggccgc tgccgggacc gttcttcctc ggtgccgggg acagcgcccg cagcgagttc
    17521 gcccccgagc gtgagttccg ccgcgatgac cgtcgccgcc gcagtcgccg tcccgaaatc
    17581 gacgatctcg cgccccgccc tcgcctgccc gaagtcagct cgcccgaagc gggcgtcaca
    17641 aatgaacgcg ctgcaagttc tggcgatgac gaaccgctcc tcgatgatga agaagccgcg
    17701 cttgccgcct tcgtagccgc catgcgcagc ggtcgagacg acgactagcg agcggtcgac
    17761 tggcgcctgg gtacgacctg gtgctacggt tcgcacatgg catcggcttc gacgcagacc
    17821 ccaaaaccgc acctggccac cttcgcggac cttttgcgca tccccgagga tcgtcgtcgc
    17881 catgaaatcc tcgacggcga aatcatcgaa aaagcgctgc cacgctggga acacggcgta
    17941 ggtcagaccc gtgctggtga gcttttgcga cgcttcaacc gcaagccgaa cggaccaacg
    18001 cgccccggcg gctggtggat actaaccgaa ccgacgatcc agctttcacg ccacgaactc
    18061 gtacaaccgg acctggcggg ttggcgacgg gatcgcgtac atgatgaacc gccgagctac
    18121 cccatcaccc tgcgccccga ctgggtgtgt gaaatcatgt ccgatggcga cgcgcgcaga
    18181 cgcgacggtc tgcaaaagcg ccgcatctat gccgaccacg acgtcccctt ctactggctc
    18241 atcgacacag agcgcgaaac cctcaccgtc ttgtccctta ccgaagagtg ctacgtcgag
    18301 cagctcaccg cctgccgcac cgaccgcgtc cgcgccgctc ccttcgacgc actcgaactc
    18361 caaatcgg
//
LOCUS       DAORGN010000091        18309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1230_length_18309_cov_6.209653, whole genome shotgun sequence.
ACCESSION   DAORGN010000091 DAORGN010000000
VERSION     DAORGN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18309
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_18309_cov_6.209653"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKL17_11495"
     CDS             complement(<1..352)
                     /locus_tag="PKL17_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45403.1"
                     /translation="MSAKTWALALALATGGALTPTAQASDHADMSAKMTTDLGTLYGP
                     RDIADLYSWVDNDPTSPNTPRRVFMILTVYPNAPKATAMFDTGTLYSFHTVSKAKMAD
                     SSATAVMPTPPQPIT"
     gene            694..2412
                     /locus_tag="PKL17_11500"
     CDS             694..2412
                     /locus_tag="PKL17_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK45404.1"
                     /translation="MTHRRQTLSSHFANRGHRPLLWAGTFALCAALLSSGCGHPEDSL
                     VHDEGGALSPLGFGEAKLAQDGTPTYLSGSMTGQVATRITTVESAAAVLTQGALASSY
                     RLAKETTLSLVSDRKDEQGNRYIKLQQKHAGLPVLGRQVVMQLGTDGTLEGVLGRLAP
                     ELVVSETAGLSAESAIAIALSSRGTGRAVTTPVLSVFIDAGGAARLAYEARVDYQSPE
                     GRKFDRMIVSAVDGQVLLSHSLIYTALGREIYDLKKTCLKDGSELPGTLLCREGGTTT
                     DASGTRAYKASGNVYWFYNKMWGRDSYDDKGSKLMSSIHATFDTGMGCSGENAAWIPD
                     PFNQMVYGDGGAFGLTLKDLTLGFDVAAHEMTHAVTAATSNLEYLDESGALNEAMSDI
                     MGAAAESWVASGGGMGGGPATWTTSANTWKIGEDVAGLLFASIGALRLMNDPTKDNMS
                     KDYYPERNMPGSADNGGVHTNSGIGNLAYYLATMGGKHPRAKTSVEVKGIGIDKATRI
                     FYLADTMLFTDKTTFQEARYATAKAAELLYGRCSTEWLTMHHAWDAVAVPGDWQPCVR
                     PRPPRF"
     gene            2419..4527
                     /locus_tag="PKL17_11505"
     CDS             2419..4527
                     /locus_tag="PKL17_11505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNK45405.1"
                     /translation="MLEKVDPTVAATAPVLVDDPFWGPRRGGAAPEPLSVSSFAAFWT
                     TPEPEVPAPAVAPIEASDAQPTVSPLPAFAQVNQAVTPLEDPPWWKVPPQIPAELQVD
                     FGIELVAGKAPLSSDDILEVARAFWSTPDDRSPALRGQEAALDKSKAAKLKAKAKPEG
                     PAPKSPPVELPSIEEEKNPVMRRIIRRLQRQFGLSGFRPGQETAIRALVEGRDVLGIM
                     PTGAGKSLIYQLTAFELPGVTVVVSPLLALMRDQEQKLRRTGVVAARLDSTLTTKETN
                     KTLDDIEEGKRKLIYVTPERAASGNLLEELGGQKVSLFVVDEAHCVSEWGHDFRPSYL
                     FLDRACEQLGRPPICALTATATPEVAQDIISQLGLKNPEETHTGFDRPSLHFEVRETS
                     DEKTQVKRLLRLIRKIQGSVIIYCSTIRTVEAIAGALPVLGLKVGMYHGKMGKAERDA
                     AQLAFMRNNPRIMVATNAFGLGVDKPDIRAVIHYNMPGSLEAYYQEAGRAGRDGRPSR
                     CILLYNPEDEQVQSFFVAGKYPNRSDVLNVWKALRNGADNLRELALAAETSQSKARVV
                     LGILKEMELSQEGPNQSFTWTGKEADELSLGRAAESYRKRREGDRSRVGAMIQFARST
                     RCRLQLLLEYFGEKAPPLCRRCDNCLNYGDDAAVERAGQLAAPLDNLDEDESTSRIVE
                     TAKAETSAAPKPPRKDSLFF"
     gene            4568..6244
                     /locus_tag="PKL17_11510"
     CDS             4568..6244
                     /locus_tag="PKL17_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45406.1"
                     /translation="MSLCWSRAVLSWSFSLLALSVCGCATPQEVTQTAVPPPQLALDE
                     IPLQITQRCPGDPACPDEGDGRLYAGFAKRDVTPQIEPFTDTNGNGVYDDGEPYEDKN
                     KNGTFDAYWIAGYDSGRLATGVNDPVWARALALRQNQTTVVVVAVDTLGLFRDEFDYV
                     NKLIDKRLGIDLLLLHGTHVHETADLTGGWGPDDFTNGVNNAYRQKVHQAIADAATEA
                     VANLRPARMTLGSIKVQDPDGNMKRYVFDSRDPVVINNVLHTMQFLDVSTTPPQPITT
                     LVNWSNHPEAAGSKNKLISSDFVHYLRQELEQKGAGPVVYVSGSIGGLLGPGHAAPLD
                     ETGTPVTEEGLKKAQALGHEVARFALTAMADPGNLSVEGKAARLSFRSTEFKTHIENQ
                     KYHLAAMLGVFKRKFCCFDDTRPLSEENIPSVETLVAYLRIGPASIITNPGELTSELF
                     LGGYDGSLAGTYPLFDPRKANAPDLSLAPLPPYLIDRMHGVREHRMTWGLTGDFLGYI
                     LPRYHFVLSEKRPYIDQAKGDHYEETNSIGPRAEAEIVGTMRQLVESDRE"
     gene            6248..7477
                     /locus_tag="PKL17_11515"
     CDS             6248..7477
                     /locus_tag="PKL17_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNK45407.1"
                     /translation="MPYHLFEPLPIGPIVLRNRTIRAAAFEGMAHGHNPTPQLVSYHR
                     AVAAGGIGMSTVAYASVTRSGLSFPHQLWLRKEIIPELSALVQAIHSEGARAAIQIGH
                     CGNMAQRRVIGERPMSASAKFNLYGPNWPRAMDKTDIAKVVHSFGQAVEIARAAGFDA
                     VEVHAGHGYLISQFLSPLTNRRSDEYGGSLRNRMRFLQQVISEVRHAAKSDLAVLCKM
                     NLRDGVKGGQDLEEAVEIAQLLEREGADALILSGGFVSAAPMYIMRGEMPTDVMSRLI
                     ENPLLRWLLRLFGSQLISSVPYRDNYFLEDAKVVRGQVKLPLICIGGVVSRAAIDEAL
                     AAGFEGVAMARALISDPAFVRKLADEERAVSNCDHCNYCAARIYTRDMACHLRQEPEP
                     QLQAICNKRGWHVRVSS"
     gene            7458..8255
                     /locus_tag="PKL17_11520"
     CDS             7458..8255
                     /locus_tag="PKL17_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK45408.1"
                     /translation="MSVSLRKTALVTGACSGIGRSLAQGLAARGYDLVLVSNRDVPLS
                     EAAKELSLQYGITALSIPMDLSSVDAAESLFQQVSQRGIEIEVLVNNAGIFFFGEVAD
                     ADPVIASRLVRLCVLTPSLLCTYFAKGMRARRSGRILMVSSISAFRDFPGIAYYGSSK
                     KYLRGFSQALRSELSVYGVSVTCLLPGATATSLYDPNVVPVELAKKLGVMADADSVSE
                     QGLAALFSGQAECIPGALNRVLSQLAESTPQPLIDLLRRHAPWLPPK"
     gene            8349..8630
                     /locus_tag="PKL17_11525"
     CDS             8349..8630
                     /locus_tag="PKL17_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45409.1"
                     /translation="MIGASVGLLGVALAWGWLVGMRMTLLRVRTLVWLGLWTLAVAFA
                     MSSLVPHPMGRASSVIWFVGSSAAACFFHLLFVSLGRELRPKRASLRDG"
     gene            8640..9086
                     /locus_tag="PKL17_11530"
     CDS             8640..9086
                     /locus_tag="PKL17_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45410.1"
                     /translation="MIDPQTVSQTVNHVVAAAPSVTQLQLLGAGCFGAIIGWYVYYIN
                     RYRKGDVQLNDVVTLIGILGGAAIQSLFKPDGALFGAYGVGLAVGFFGYFFVLIILVG
                     VSKNFDVDWFLDGRRRKPSGEYEIPDSVRASVSAMDSKPKNGVDIS"
     gene            9112..11706
                     /locus_tag="PKL17_11535"
     CDS             9112..11706
                     /locus_tag="PKL17_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNK45411.1"
                     /translation="MADSVASYSADFSTLLALILDGLCDGDGGRIRIEFLSDLAPLWP
                     VDLIPSAAEAVRDLGLPDRETAQTIEALVSYLDVLQADLFVEESRALRPSLAPHFGAA
                     DSAFFLRQKTSVQTAVDAAQIRARRIVICRPIQQELARKQIALDSLPLLPPLPIRGLR
                     FCIRQLALDEERRLLFELVTTLLVRTEALSKRARPGALGVSVPSARGSNAVNYSVPFP
                     AQISFRDVAPPTSSNPFAGDEPDFSPSADDVMSLQTEDTSPPVISTGFSDADTPQTPI
                     PPQRPLFPDRGYYFWLQLGKRVSDSIELQHIGLPKELVHEGALLDVVLFSFSHELELT
                     PSACIGTLRVESDGSARVVSQPGISTDGKTPFGSDGLRRLFFPLRTPPAVQAGTVLHL
                     RCSIYCNQVLLQSRLISAELGNPQETSLPQADAPRLRSLLDYSLIDTVASPDSLRRIT
                     PHKLSLFFNDNPDGTHSFRFFAKEGNRQLRHEVTLDRDRLSAQIQRARGELRRAAWGD
                     TGPWKGPSGGQSYRYADQPRSVLREQLYSDLIRLAKSGATLYAEMARHLTSSVEESFE
                     LADLMRTPGFLQIASKVTATQTVPAALLYDQPLSVNPTEDKFTICDAFRSALESAQPL
                     DQCPCFLGNCPSYDRDTVVCPSGFWGFRHFLGMPLTGPNRSEAQPQILYHDAPRLSVG
                     VSLELHEFPVHEKALRNLRSDLQWQCESSAQKIFQMLKEGKSHLVYFYCHGGESPDLP
                     FLEVGNDEYIVPRALMRYRIRWQDPRPIVFLNGCHTTAVDSEQVMDLVSEFVEIGQAS
                     GVIGTEITIFEPMARAFAEECLRRFFAGQPIGQSIRSARLSLLKQCNPLGLAYDPYVL
                     ATVCLAKQ"
     gene            complement(11727..12239)
                     /locus_tag="PKL17_11540"
     CDS             complement(11727..12239)
                     /locus_tag="PKL17_11540"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNK45412.1"
                     /translation="MVRAAFTLLLALVLCAVSAQAAAPADGKAAAPVAKTEPKTIDPA
                     KRADILRLLQVTQAASMGEQAVQQILATFRQMMPQVKDEVWQEVKKEVSADELIQRLV
                     PIYDKHFSPAELKDLLRFYESPIGKKTTQVMPAINQEALQIGQQWGSEVSLRVKRRVD
                     ALSPLPAAKK"
     gene            complement(12233..12952)
                     /locus_tag="PKL17_11545"
     CDS             complement(12233..12952)
                     /locus_tag="PKL17_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK45413.1"
                     /translation="MDETSSEAGQPAIGTVLVTGASRGIGLDFVRAWLERGAHVIAGC
                     RNPTSASALGTLMSEYGERLRVMQLDVVDERSVETMAGAIGNQPIDLLINNAGILQVE
                     TLENMDFASILDQFKVNALGALHVTRSLLSNLRDGAKIINITSRMGSLDDNTSGGYYG
                     YRMSKAALNMATRSLAVDLKNRGIIVVAMHPGMVQTDLTRGFGMLTAAESVAGMNQVI
                     DKLTIEQSGLFLHYQGTTLPW"
     gene            complement(12997..13704)
                     /locus_tag="PKL17_11550"
     CDS             complement(12997..13704)
                     /locus_tag="PKL17_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNK45414.1"
                     /translation="MHKIRRLCDFVGVKGRSSDHAAQPEDIRPSQRAAQSKSQPNGSR
                     RPGATVLRRRPRKHRAQPVRGLSLLASGADRLGLWPKLWEQWVCRTFSLTLQACVPRL
                     ATMARAEKLADGTLTVVVSSSAVASELSFVRDLLLEQINRELLSAVSLLDEKQKRQVR
                     PVLRIQNRVGKLSGLPDPMEWQERPTVTKRPPPPAPSLESQLAVAEASQGMHDDELRL
                     LMLRLVGAYKGVDSAKP"
     gene            13777..15810
                     /locus_tag="PKL17_11555"
     CDS             13777..15810
                     /locus_tag="PKL17_11555"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK45415.1"
                     /translation="MKKQKSAFRCAMGFMLLVAGCSSGGVPGPGMTSGQVDMAVGDNP
                     DGGGDPSKDMSGQPPEDMAGMPPVDLAYTSGLPCTPGTYRCGAGLSVEICNATGTAWL
                     YSATCTVGCMSGLCTGACTPSAKRCNAKNVETCNPTGSAWMVSETCSTFCEDARCALP
                     SLDVGANKTLDGDQLIDGDVIIRSGATLSSPTGNLSIRTRGSITVEMGASIVVNPTGA
                     SPDGQGGCYYYYSSYNGAGGGGYGSNGTAGSGGYYGGAAWGSTTDINVSSGSMGGTSC
                     GSYGGTRGGKGGGMLRLMAAKNITIDGQVTANGESGNSSSYGGGGGGSGGGILIAAQK
                     KVTVTGSVSAAGGSGGAGAASYYNGGNGGQGRVKILNSGDRSITGTINGVRTDGLLPP
                     LSISSSSHPDQALYYNDDSPGLALAWEQAFAGRQGYYWAVNTTGSLPPTPANGGKVVA
                     MEMVTAAVSSLTAGTNYFHIVPIDAMSNVGTVENIFKVNINSTPPTLRSSSHPSSGTW
                     YASGDVFFEWTFPQDDKNFKGIYYVLDHYGETLPAASMATMVPSTQKQLLRSGLANGV
                     WVMHMVPIDQRGYLTKTASHYRVFVGTDPGKGGLLGQVVDAMGKPVSGAKVTVNRGLW
                     NATTNASGSYNFMATIPTGTWEVRAISPDGSLTETKMDMVTKDGTTTVNFTLK"
     gene            15905..16297
                     /locus_tag="PKL17_11560"
     CDS             15905..16297
                     /locus_tag="PKL17_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45416.1"
                     /translation="MKPRRTRSTSWISIGVAAIAVAWLSGCCSNVRCIPCQPAGIYLT
                     VLDDWTQMVQSAAKVSIAGKPCQPASVKGSAVYICSVPAGTYSIDIEQPGYTPTQVMF
                     ALEAEGDGCCLCGPIAYGSASIVPVETM"
     gene            complement(16316..17713)
                     /gene="hydA"
                     /locus_tag="PKL17_11565"
     CDS             complement(16316..17713)
                     /gene="hydA"
                     /locus_tag="PKL17_11565"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNK45417.1"
                     /translation="MVTVIKNGTLVTASDTYQANLWIDGEKIVSHTHPSQPIPTGSDV
                     KVIDASGCYVIPGGIDAHTHLDMPFGGTTSSDDFLTGTRAAAYGGTTTIIDFAIQQKG
                     GTLRQALDTWHAKAAGKGCIDYGFHMIATDAPQSVLTEMGDIVSEGVTSFKLFMAYPG
                     VLLLDDQSIFRCMLRAGEIGALICMHAETGLPIDVLVERAVAKGHTAPIYHALTRPEV
                     AEATGTERAIALAEMAKVPVYIVHLSAARALERVREARDRGLPAYAETCPQYLFLSDD
                     ALRGTPTDPFDGAKYVCTPPLRPASHHEHLWRGLRNYDLQVVSTDHCPFCMKDQKVLG
                     RDSFAKVPNGMPGIETRLHLLFEAVAEGKLSLNRFVEIASTAPAKIFGLYPKKGSIAV
                     GSDADVVVWDPKRALKLSHENLHMRVDYAAYPDKTVPGSPRFVFSRGELVIDQGQHVS
                     RPGHGRFVRRSTFSL"
     gene            17712..17834
                     /locus_tag="PKL17_11570"
     CDS             17712..17834
                     /locus_tag="PKL17_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45418.1"
                     /translation="MFPPGSRHRSAEPLDVKTPKIAALAGTFYKAPCPPLAKML"
     gene            17860..>18309
                     /locus_tag="PKL17_11575"
     CDS             17860..>18309
                     /locus_tag="PKL17_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45419.1"
                     /translation="MRPLRIGLCGLLLLASPRAMSAVPVGLRSSTVKPATQYINNLPP
                     PPQPPPPPPSVMDGQKDQLIERDVVRRVIYLHIKQIRNCYQQALFKNPKLAGRLVGQW
                     GEPRQCLPRQSRRRGSPGLWRNARRLRGGAGDPGDEGARVARHLLSLP"
BASE COUNT         3461 a         5351 c         5650 g         3847 t
ORIGIN      
        1 cagtgatcgg ctgcggcggc gtgggcatga cggcggtggc gctgctgtcg gccattttgg
       61 ctttcgaaac cgtgtggaag ctgtacagcg tgccggtgtc gaacatcgcc gtcgccttgg
      121 gggcgttcgg atagacggtg aggatcatga agacccgccg cggtgtgttc gggctggtgg
      181 ggtcattgtc gacccatgaa tagaggtcgg cgatgtctcg cgggccatac agcgtgccga
      241 gatcggttgt catcttggcg gacatgtccg cgtgatcact ggcctgggcg gttggggtca
      301 gcgccccgcc ggtggccaga gcaagggcta gtgcccacgt cttcgcgctc atgctgggtc
      361 tcctgtctaa agcctcgtcg ctaggtcgaa tacagactta ggtcgtatca gcttatcaca
      421 ggtggctttg cgggaagaaa ctcgattcgt cgcacacttc gccgcgttgg cggggatgcc
      481 cggaacggct tgtcttgtcg catttcgcga tcgcggtgag ccatggcgcg cttttttgga
      541 cagagtgctc gacgaagcac caaaaatatt ggggtgaagc ggcaggttgg cttacataag
      601 cgcaagaaaa ccttcttctt tccggtcgcg gttgcgcctc tgtttgagta ggcttgcgcc
      661 gattcaccgg cccaacacac taaggagtct tacatgaccc atcgtcgtca aacactctct
      721 tcacactttg ccaaccgggg acatcggccc cttttgtggg ctgggacatt cgcgctttgt
      781 gcggcccttc tgtcgagcgg ctgtggccat cccgaggact cgctggtcca cgatgagggc
      841 ggggcgctgt ctccgctggg ttttggtgaa gccaagctgg cgcaggacgg caccccgacg
      901 tatctgtcgg gttcgatgac cggccaagtg gcgacgcgga tcaccacggt cgaaagcgcg
      961 gcagcggtcc tgacgcaggg ggctttggcg tcgagctatc gactggcgaa agaaacgacg
     1021 ctttcgcttg tgtcggatcg caaagacgag caaggcaatc gctacatcaa gcttcagcag
     1081 aaacacgcgg gtctgccggt tctcggtcgg caggtggtga tgcagcttgg caccgatggg
     1141 acgcttgagg gtgtccttgg tcggctggcg cctgagcttg tggtttcgga aaccgcgggt
     1201 ctgtcggcag aatcggcgat cgccattgcg ctgtcgtcgc gaggaacggg ccgggcggtg
     1261 acgactccag ttctgtcggt gttcatcgat gccggcgggg cggcgcgtct cgcatacgag
     1321 gcacgggtcg attatcagtc ccccgaaggt cgcaagttcg atcgcatgat cgtaagcgcc
     1381 gtcgacggac aggtcctgct ttcgcactcg cttatctaca ctgcgctcgg acgcgagatt
     1441 tacgatctca agaaaacctg cctcaaggac ggctcggagc tgccgggcac gctgctctgt
     1501 cgcgaaggcg gaaccaccac cgatgcgtcg ggcactcgcg cctacaaggc cagcggcaac
     1561 gtctactggt tttacaacaa gatgtgggga cgggattcct acgacgacaa aggctcgaag
     1621 ctgatgtcga gcattcacgc cacgtttgat accggcatgg gatgcagtgg tgaaaatgcg
     1681 gcctggattc ccgatccgtt caaccagatg gtgtatggcg acggtggtgc gtttgggctg
     1741 acgctcaagg atctgacgct cggtttcgat gtcgcggcgc acgaaatgac gcacgcagtg
     1801 acggcggcca cgtcgaatct ggaatacctg gatgagtcgg gcgcgctcaa cgaagcgatg
     1861 tccgatatca tgggcgcagc cgcagagtcg tgggttgcgt cgggcggcgg catgggtggc
     1921 ggtccagcga cgtggacgac cagcgcgaac acctggaaaa ttggtgagga tgtcgcaggt
     1981 cttttgttcg cgtcgatcgg cgcgctgcgg ctcatgaacg atccgaccaa ggacaacatg
     2041 agcaaggact actaccccga gcgcaacatg cccggttcgg cggacaacgg tggtgttcac
     2101 accaattccg gcatcggcaa cctggcgtac tatttggcga cgatgggcgg caaacacccg
     2161 cgcgccaaga ccagtgtcga ggtcaagggc atcggcatcg acaaagcgac gcggattttc
     2221 tacctggccg atacgatgct gttcaccgac aagacgacct tccaagaggc gcgttacgcg
     2281 acggcgaaag ctgccgagct gctctatggt cgttgcagca ccgagtggct gacgatgcat
     2341 catgcctggg atgccgttgc tgtgccgggt gattggcagc cgtgcgttcg tccgcggcct
     2401 cctcgcttct aaggaaacat gctggaaaaa gtcgacccga ccgtggctgc gaccgctcct
     2461 gtcctcgtcg atgacccgtt ttggggtcca cggcggggcg gggcggcgcc tgagcctctt
     2521 tctgtttcgt cgtttgccgc cttttggacg acgccagagc ccgaagttcc ggctccagcc
     2581 gtcgctccga tcgaggcaag cgatgcgcaa ccgactgtga gcccgcttcc tgctttcgca
     2641 caagtgaatc aggcggtgac gccgctggaa gatccgccgt ggtggaaagt tccgccgcag
     2701 attccggcag agctacaggt cgactttggc atcgagctag ttgccggcaa agcaccgctg
     2761 tcgtctgacg acatcctcga ggtggcgcgg gcattttggt cgacgcccga cgatcgttcg
     2821 ccggcgctgc gtggtcagga agcggcgctg gacaagagca aagccgccaa gctgaaagcc
     2881 aaggccaagc ccgaaggtcc agcacccaag agcccgccgg ttgagctgcc gtcgatcgag
     2941 gaagaaaaaa acccggtcat gcggcggatc atccgtcgtc tccagcgcca gttcgggctg
     3001 tcgggtttcc gtccgggtca ggaaaccgcg attcgtgcgc tcgtcgaagg tcgcgatgtt
     3061 ctgggcatca tgccgacggg tgccggcaag tcgcttatct atcagctcac cgcgtttgag
     3121 ctgccgggcg tgacggtggt tgtgtcaccg ctgctggcgc tgatgcgcga tcaagaacag
     3181 aagctgcggc ggacgggtgt ggttgcggca cggcttgatt cgacgctgac caccaaagaa
     3241 accaacaaga cgctcgacga catcgaagaa ggcaagcgca agctgatcta tgtgacgccg
     3301 gagcgggccg cgtcgggcaa cttgctcgaa gagctgggcg gacaaaaagt cagtctgttt
     3361 gtcgtcgacg aagcgcactg cgtgtccgaa tggggtcatg acttccgacc gtcgtacttg
     3421 ttcctggaca gggcgtgcga gcagctcggg cggcccccga tttgtgcgct gacagcgacg
     3481 gcaactcccg aagttgcgca ggacatcatt tcgcagcttg gtttgaaaaa ccccgaagag
     3541 acgcacaccg gatttgatcg tccgtcactg cacttcgagg tccgcgaaac atccgacgaa
     3601 aaaacccagg tcaaacggct gctgcgcctg attcgcaaga ttcagggttc cgtgatcatc
     3661 tattgctcga cgattcgtac cgtcgaagcg atcgccggag cgctgccggt gctgggcctg
     3721 aaagttggca tgtaccacgg caaaatgggc aaagccgagc gtgacgcggc gcagctggcg
     3781 tttatgcgca acaacccgcg catcatggtc gcgaccaatg cgttcggtct gggcgtcgac
     3841 aaacccgaca ttcgcgccgt cattcactac aacatgccgg gctcgctcga ggcgtattac
     3901 caggaagcgg gtcgagcagg ccgcgatggc cgtccaagtc gctgcatcct gctttacaac
     3961 ccagaggacg agcaggttca gagctttttc gtcgctggga agtatccaaa tcgcagcgat
     4021 gtgctcaatg tttggaaagc gctgcgcaac ggggccgaca acttgcggga actggcgctg
     4081 gccgctgaaa catcccagtc caaggcgcgc gtcgtcctcg ggatcttgaa agaaatggag
     4141 ctttcgcaag aaggcccaaa ccagagcttt acttggactg gcaaagaagc cgacgagctg
     4201 tcgctgggcc gcgccgccga gtcatatcgc aagcgccgcg aaggcgatcg cagccgtgtc
     4261 ggcgccatga ttcagtttgc ccgctcgacg cgctgtcggc ttcagctctt gctggagtat
     4321 ttcggtgaaa aagcgccgcc gctgtgccgt cgctgtgaca actgtctaaa ctacggtgac
     4381 gatgccgcgg tcgagcgagc cggtcagctt gccgcaccgc tcgacaatct cgatgaagac
     4441 gagtcgacat cgcgcattgt ggaaaccgcc aaagccgaaa cctcggcggc accgaagccg
     4501 ccccgcaaag actcgctgtt cttttagccc acctgttttc ctttcttttc gccgagcgag
     4561 gtgtgccttg tcgctttgct ggtcccgcgc tgttctttcg tggtcgtttt ccctgctggc
     4621 gctttcggtc tgtggctgtg cgacgccgca agaagtgacg cagacggcgg ttccgccgcc
     4681 gcagcttgcg ctcgacgaga ttccgctcca aatcacccag cgctgtccgg gcgatccggc
     4741 gtgtcccgat gagggtgatg gacgcctgta cgccggtttt gccaagcgcg atgtgacacc
     4801 gcagatcgag ccgtttaccg atacaaacgg caatggtgtc tatgacgacg gggagcccta
     4861 cgaagacaaa aacaagaacg gcacattcga tgcgtattgg attgccggct atgacagcgg
     4921 acggctggcg accggggtca atgatccggt ctgggcccgc gcgctggcgc tgcggcaaaa
     4981 ccagacgacg gttgtggtcg tggcggtcga tacgctcggg ctgtttcgcg atgagttcga
     5041 ttacgtaaat aagctgatcg ataagcgttt gggaatcgac ctgctgctgc tgcacggtac
     5101 acacgtccat gagacggcgg atctgacggg tggctggggg ccggacgact ttacaaacgg
     5161 cgtcaacaac gcctatcggc aaaaagttca ccaggcgatt gcggatgccg cgacggaagc
     5221 ggtggccaat ctgcggcctg cgcggatgac gctcggttcg atcaaagtgc aagatcccga
     5281 cggcaacatg aagcgctatg tctttgactc gcgcgatccg gtcgtcatca acaacgtgct
     5341 gcacacgatg cagtttctgg atgtcagcac gacgccgccg cagccgatta ccacgctggt
     5401 caactggtca aatcaccccg aggctgcggg cagcaaaaac aagctcatct catcagactt
     5461 tgttcactat ctgcggcagg agctggagca aaaaggcgct gggccagtgg tctacgtttc
     5521 gggatcgatt ggcggtctgc tggggcccgg acatgcggcg ccgctcgacg agacggggac
     5581 tccggtgacg gaagaggggc tgaaaaaagc gcaggcgctc gggcatgaag tggcgcgctt
     5641 tgcgcttacg gcgatggctg atccgggcaa cctttcggtc gaagggaaag cggcgcggct
     5701 cagcttccgg tcgacggagt ttaagaccca catcgaaaac caaaagtacc atctggcggc
     5761 gatgctcggg gtgtttaagc gcaagttttg ctgcttcgat gacacccgtc cgctgtcgga
     5821 agagaacatt ccttccgtcg aaacgctggt ggcctatctg cgcatcgggc cagcatcaat
     5881 cattaccaat ccaggcgagc ttacgtccga gctgtttttg ggcggctacg atgggtcgct
     5941 ggcgggcacc tatcctttgt ttgatccgcg caaagccaat gcgccggacc tctcgctggc
     6001 tcctctgcct ccgtatctca tcgatcggat gcacggagtt cgtgaacatc ggatgacgtg
     6061 gggactgacg ggcgattttc tcggctacat cttgccgcgc tatcactttg tgctgtccga
     6121 aaagagaccc tacatcgatc aagccaaggg tgatcactac gaagagacga actcgattgg
     6181 accgcgcgcc gaggctgaaa tcgtcggtac gatgcggcaa ctggtggaat ccgatcgcga
     6241 gtaagacgtg ccgtaccatc tgtttgaacc gctcccaatc ggtcccattg ttctgcgcaa
     6301 tcgcacgatt cgcgccgcgg cgtttgaagg aatggcgcac ggacacaatc ccacgccaca
     6361 gcttgtttcc tatcatcgcg cagtggccgc gggtggaatc ggaatgtcga cggtggcgta
     6421 tgcctcggtg acgcgcagcg ggctgtcttt tccgcaccag ctttggttgc gcaaagagat
     6481 cattcccgag ctgtcagcgc ttgtgcaggc catccattcc gaaggcgcgc gggccgcgat
     6541 acagataggc cactgcggca acatggccca gcggcgtgtg atcggtgaaa gaccaatgtc
     6601 ggcgtcggca aagttcaatc tgtatggtcc gaactggccg cgtgcgatgg acaaaaccga
     6661 catcgcaaaa gtagtccaca gctttggtca agccgtagaa atcgcgcgcg cagcgggctt
     6721 tgatgcggtc gaagtccacg caggacacgg ctatctgatt agccagtttc tgtctccgct
     6781 cacaaaccga cgcagcgacg agtatggcgg ctctctgcgc aatcggatgc gctttttgca
     6841 gcaggtgatt tcagaagtcc gtcacgcggc caaaagtgat ctcgcagtgc tttgcaaaat
     6901 gaacctgcgc gatggcgtaa aaggcggaca agaccttgaa gaggccgtcg aaatcgccca
     6961 gcttttggaa cgtgaaggcg ccgatgcgct gattctgtcc ggcggctttg tgagcgcggc
     7021 accgatgtac attatgcgcg gtgaaatgcc gaccgatgtg atgtctcggc tgattgaaaa
     7081 tccgctgctg cgctggcttt tgcgcctgtt tggttcgcag ctgatttcgt cggttccgta
     7141 ccgcgacaac tactttcttg aagatgcgaa agtggttcgc ggtcaggtga agttgccact
     7201 catttgcatt ggcggggtgg tgtcacgcgc tgcgattgat gaagcactgg ccgcgggttt
     7261 tgaaggcgtt gcgatggcgc gcgcgctgat ttctgatccg gcctttgttc gcaagctggc
     7321 cgatgaggaa cgagcggtat cgaactgcga tcactgcaac tactgcgcgg cacgaattta
     7381 cacccgcgac atggcgtgtc acttgcggca agaaccagag ccgcagctgc aagcgatttg
     7441 caacaaaaga ggatggcatg tccgtgtctc ttcgtaagac ggcgcttgtc actggagctt
     7501 gttccggaat cggtcgctcg cttgcacagg ggttggcggc gcgcgggtac gacttggtcc
     7561 tggtcagcaa ccgtgatgtt ccgctttccg aagccgccaa agagctgtct ttgcagtacg
     7621 gaatcaccgc gctgtcgatt cccatggatc tctcttctgt cgacgctgcc gaatcgcttt
     7681 ttcagcaggt ttcgcagcgt ggcatcgaga tcgaagtcct ggtcaacaac gccggcattt
     7741 tctttttcgg agaggttgca gatgccgatc ccgtgatcgc ttcgcggctt gtgcggctct
     7801 gtgtcctgac gccttcgctt ttgtgtacgt actttgcaaa aggaatgcga gctcgacgga
     7861 gcggacgcat tttgatggtg tcgagcattt ctgcgttccg tgattttccg ggcatcgcgt
     7921 actacggcag cagcaaaaaa tatctgcgcg gtttttcgca agcgctgcgc agcgagctgt
     7981 ctgtgtacgg tgtctctgtg acctgtcttt tgcccggtgc caccgcgacc tcgctgtacg
     8041 atccgaacgt agttcccgtc gaactggcga aaaaactggg cgtcatggcc gacgctgatt
     8101 ctgtgtccga gcaaggcctt gcggcgctgt tttccggaca agcagagtgc attccggggg
     8161 ctctcaatcg tgtgctgtcc cagcttgccg aatcgacgcc gcagccgctg attgatctgc
     8221 tgcgtcgtca cgcgccctgg ctgccgccca agtagtccga agctccggtg cggcctgcgg
     8281 acttgccccc gttcgaggcg gaaagtgcgc gcttgcctcg atcctgtaat ccttctacgc
     8341 tagaagggat gatcggcgca tccgtcgggc tgcttggggt tgcccttgcg tggggctggc
     8401 tcgtcggaat gcgcatgaca ctgctgcggg tacggacgct tgtgtggctg ggtctgtgga
     8461 cgctggctgt ggcgtttgcg atgtcatcgc ttgtgccaca tcccatgggc cgcgcctcgt
     8521 ctgtgatctg gtttgtggga agctccgcag ccgcctgttt ttttcacctg ttgtttgtgt
     8581 cgctcggaag agagctgcgg ccaaagagag cctctcttcg cgatggatag gaatgcacca
     8641 tgatcgatcc ccagacggtt tcccaaactg tgaaccatgt cgtcgctgca gcgccttctg
     8701 tgacccagct tcagcttttg ggagcaggtt gttttggcgc catcatcggc tggtatgttt
     8761 actacatcaa tcgctaccgc aaaggagacg ttcagctaaa cgatgtcgtg acgctgattg
     8821 gcatcttggg gggcgctgcg attcagtcgc tgttcaaacc cgatggcgca ctgtttggtg
     8881 cgtatggagt cggtctggcg gtcggttttt ttggttactt ttttgtgctg atcatcttgg
     8941 tcggtgtgtc caaaaacttt gatgtggact ggtttctcga cggaagacgc aggaaaccaa
     9001 gcggcgaata tgaaattcca gattccgttc gagcctctgt gtcggcgatg gattccaagc
     9061 ccaaaaatgg tgtggatatc tcgtaggaat cctttggggg atgatccgaa catggcggac
     9121 tcagtcgcaa gctacagcgc agacttttct actcttttgg cgttgattct ggatggactg
     9181 tgcgacggcg atgggggtcg catccggatc gagttccttt ctgatttggc accgctttgg
     9241 cccgtcgatc tgattccctc tgcggcggaa gctgttcgtg atctgggtct tcccgatcga
     9301 gagacggccc agaccattga agcgcttgtg tcgtacctgg atgtgctgca agccgatctg
     9361 tttgtcgaag agtcgcgcgc actgcgtcca agcctggctc cgcattttgg tgccgccgat
     9421 tctgcctttt tccttcgtca aaaaacgtcg gttcagacgg ctgtggacgc ggcgcagatt
     9481 cgcgcgcggc gcattgtgat ctgtcgtccg atacagcagg aactggcccg caaacagatt
     9541 gcgctggatt cccttccgct tctgccgccg cttcccatcc gtggtctgcg tttctgtatc
     9601 cgccagcttg cgctcgacga ggaacggcgg ctcctgtttg aacttgtgac cacgctgctg
     9661 gttcgtaccg aagccttgtc caaacgggcg cgacctgggg cacttggcgt ctctgtgccc
     9721 tctgcgcgcg gatcgaatgc tgtaaattac tcggttccgt ttccggcgca gatttcgttt
     9781 cgcgatgtgg ctcccccgac cagcagcaat ccgtttgcgg gggatgaacc agacttttcc
     9841 ccgagtgcag acgacgtgat gagtctgcaa acagaagaca cgtccccgcc tgtgataagc
     9901 accggatttt ccgatgcaga cacaccgcag acaccgattc cgccgcagcg acctctgttt
     9961 cctgatcgcg gctactattt ttggctacag cttggcaaaa gggtgtccga ttcgatcgaa
    10021 cttcagcaca tcggtcttcc caaagagctg gtacacgaag gcgcgctgtt ggatgtggtg
    10081 ctgttttcgt tttcacatga gcttgagctg accccttctg cgtgcatcgg aaccctgcgg
    10141 gtcgaatcgg atggaagcgc gcgcgtagtg tcccaaccag gcatatctac cgacggaaaa
    10201 acgccgtttg ggtcagatgg actgcggcgc ttgttttttc cgctgcggac tcctcctgct
    10261 gtacaagcgg ggaccgtgct gcatctgcgc tgtagcatct attgcaacca agtattgctg
    10321 cagtcgcgct tgatttccgc ggagcttggg aatccgcaag agacttcgct tccgcaggca
    10381 gatgcgccgc ggctgcgctc tcttttggac tacagcttga tcgatacggt ggccagtccg
    10441 gattcgttgc gccgcatcac accgcacaaa ctgagtctat tttttaacga caatcccgac
    10501 gggacacaca gcttccgttt ttttgccaag gaaggaaacc gccagctccg tcatgaagtc
    10561 acgctggatc gcgacagact gtctgcacag attcagcgtg cgcgtggcga gctgcggcgt
    10621 gcagcgtggg gagacacagg tccgtggaaa ggtccaagcg gaggacagtc gtatcgctac
    10681 gcagatcagc cgcgcagcgt gctgcgggaa cagctctatt ccgatctgat ccggcttgcg
    10741 aaaagtgggg caacacttta tgccgaaatg gcgcgacacc tgacatcgtc ggtggaggag
    10801 agctttgagc tggccgattt gatgcgcacg cccggctttt tgcagatcgc aagcaaagtc
    10861 accgcgaccc agactgtgcc cgcagcgctg ctttatgatc agccgcttag cgtcaatccc
    10921 accgaggaca agtttacgat ctgtgatgca ttccgatcgg cgctggagtc cgcgcagccg
    10981 ctcgatcagt gtccctgttt tttgggaaac tgtccttcct atgatcgcga cacggtggtc
    11041 tgtcccagtg gcttttgggg attccgtcac tttctgggaa tgccgctgac cggaccgaat
    11101 cgcagcgaag cgcagccgca gattctgtat cacgatgcac cacgtctgtc cgtcggtgtt
    11161 tcgctggagc tacacgagtt tccggtccac gaaaaagcgc tgcggaatct gcgcagcgac
    11221 ttgcagtggc agtgtgaatc gagcgcgcaa aagatttttc aaatgctcaa ggaagggaag
    11281 tcacaccttg tgtacttcta ctgtcatggc ggagagagtc cggacttgcc ctttttggaa
    11341 gtcggaaacg atgaatacat cgtgccgcgt gcgctcatgc gctatcgcat ccgctggcaa
    11401 gacccgcgtc cgatcgtgtt tctgaacggc tgtcacacca ccgcggtcga ttccgaacag
    11461 gtgatggatc tggtgtcgga atttgtcgaa attggtcaag ccagcggcgt gataggaacc
    11521 gagatcacca tctttgaacc gatggcgcgc gcctttgccg aggagtgtct gcgccgcttt
    11581 ttcgccggtc aaccgatcgg acagagcatc cgcagcgccc gattgagtct tctgaagcag
    11641 tgcaatccgc tcgggctcgc ctacgatccg tatgtgctgg ccaccgtctg tttggcaaaa
    11701 cagtaaaggc agggaacgga tcggcgctac tttttggccg cgggcaaagg actgagcgca
    11761 tcgacgcggc gcttgacccg cagcgacact tcgcttcccc actgctggcc aatctggagc
    11821 gcttcttgat tgatcgcggg catcacctgc gtggtttttt tgccaatcgg tgactcgtaa
    11881 aatcgcagca gatccttaag ctcggcgggg gaaaagtgct tgtcgtaaat cgggaccaga
    11941 cgctggatca gctcgtcggc actgacttcc tttttgacct cttgccagac ctcgtctttg
    12001 acctgcggca tcatctggcg aaacgtcgcc aaaatctgct gcaccgcttg ttcccccatg
    12061 ctcgcggcct gcgtcacttg cagaagccgc aagatgtcgg ctcgcttggc ggggtcgatg
    12121 gtctttggct ctgtcttggc aaccggggct gctgctttgc catcggctgg ggccgccgcc
    12181 tgcgcagaaa ccgcacacag gaccagcgcc aaaagcagcg tgaaggcagc ccttaccacg
    12241 gcagcgtcgt gccttggtag tgcagaaaca gaccgctctg ctcgatggtc agcttgtcga
    12301 tgacttggtt catcccggcg acgctctctg ccgccgtcag catgccaaaa ccgcgcgtca
    12361 ggtcggtttg caccatgccc ggatgcatcg ccacaacgat gatgccgcgg ttttttagat
    12421 ccaccgccaa gctgcgggtc gccatgttga gcgccgcttt gctcatccgg tagccgtaat
    12481 aaccacccga tgtgttgtca tcgagcgagc ccatccgcga cgtgatgttg atgatctttg
    12541 caccgtcgcg caggttcgac aaaagcgacc gtgtcacatg cagcgccccg agtgcattga
    12601 ccttgaactg gtcgaggatg ctggcaaagt ccatgttttc cagcgtctcg acttgcagaa
    12661 tgccggcgtt gttgatcagc aaatcgatcg gttggttgcc gatggcacct gccatggttt
    12721 cgaccgatcg ttcgtcgaca acatcgagct gcatgacacg cagccgctcg ccgtattcgc
    12781 tcatcagcgt accgagcgcc gatgcacttg tgggattgcg acagccggcg atgacgtggg
    12841 caccgcgctc gagccaggca cggacaaagt ccagcccgat tccccggctg gctcctgtca
    12901 ccagcaccgt gcctattgct ggttgacccg cttcactgct tgtttcgtcc atacctgacc
    12961 tttgtagggc aaaccgcgtc ccggctgcaa gcggcgttat ggcttcgccg agtctacgcc
    13021 tttatacgcg cccaccagcc gcagcatgag aagccgcagc tcatcgtcgt gcatgccttg
    13081 gctggcctct gcgaccgcga gctggctttc caggctcggc gcaggcggcg gcggtcgttt
    13141 cgtcaccgta gggcgctctt gccattccat cgggtcgggc agcccgctca gcttgcccac
    13201 ccggttttga atccgcagca ccggacggac ctggcgtttt tgtttttcgt cgagaagcga
    13261 caccgccgaa agcagctcgc ggtttatctg ttcaagcaaa aggtcgcgca caaacgacag
    13321 ctcactggcc actgccgacg atgaaaccac caccgtgagc gttccatccg cgagcttctc
    13381 ggcccgcgcc atcgtcgcaa gccgcggtac acaggcttgc agcgtcaggg aaaacgttcg
    13441 gcacacccac tgttcccaga gcttaggcca cagccccagt cgatcggcac ccgacgccaa
    13501 aagcgacaga ccacgcaccg gctgggcgcg atgtttgcgg ggccgtctgc gcagcacggt
    13561 cgctccggga cgtcggctgc cgttcggctg actcttggac tgcgccgccc gctgagacgg
    13621 acggatgtct tcgggctggg cagcgtgatc ggaagaacgg cctttcacgc ccacaaagtc
    13681 gcacaaccgg cggattttgt gcacttttcc tctcaacgga aatggcaggg ttcgttataa
    13741 gaggcggcac acagactgtg aaatgagagg aaaaacatga agaagcaaaa atctgcgttt
    13801 cgatgcgcga tggggttcat gctcctcgtc gctggctgtt ccagtggcgg cgttccgggc
    13861 cccggaatga cgagcggcca ggtcgacatg gcggtgggcg acaaccccga tggtggcggc
    13921 gatccgagca aggacatgtc cggccagcct cccgaagaca tggcgggaat gccgccggtc
    13981 gatctggcgt acacgtcagg tctgccctgt acgccgggta cgtatcgctg cggcgccggg
    14041 ctttcggttg aaatctgcaa tgcgactggc acggcgtggc tctattcggc gacctgcacc
    14101 gtcggctgca tgagcggact ttgcaccggc gcatgcaccc caagcgccaa gcgctgcaac
    14161 gccaagaacg tcgagacctg caacccgacc ggcagcgctt ggatggtcag cgagacgtgc
    14221 tcgactttct gtgaagatgc tcgctgtgcg ctgccgagcc tcgacgttgg cgcgaacaag
    14281 accctcgatg gcgaccaatt gatcgatggc gatgtcatca tccggtcggg cgcgacgctg
    14341 tcttcaccga cgggaaatct gtcgattcgc acccgtggtt ctatcaccgt cgaaatgggc
    14401 gcgtccatcg tcgtaaaccc aaccggagcg tcgccggacg ggcagggcgg ttgctactac
    14461 tactactcgt cgtataacgg cgcgggtggt ggtggttatg gctcgaacgg caccgcggga
    14521 tccggtggat actatggcgg cgcggcgtgg ggctcgacga cagacatcaa cgtctcttcg
    14581 ggcagcatgg gcggcacgtc gtgcggtagc tacggcggca cccgtggggg caagggcggc
    14641 ggcatgctgc gcctgatggc ggccaagaac atcaccatcg acggccaggt cacggccaac
    14701 ggtgaatcgg gcaatagttc gtcgtatggc ggcggtggtg gtggatcggg tggtggcatc
    14761 ctgatcgctg cgcaaaagaa agtcaccgtg accggatcgg tgtcggcagc cggcggatcg
    14821 ggcggtgctg gcgccgcgtc gtactacaac ggtggcaacg gcggtcaagg ccgcgtcaag
    14881 atcctcaaca gcggcgatcg cagcatcacc ggcacgatca atggcgtcag gaccgacggt
    14941 cttttgccac cgctgagcat ctcttcttca tcgcatcctg accaggcgct ctactacaac
    15001 gatgactcgc cgggcctggc gctcgcgtgg gagcaagcgt tcgcaggacg tcagggctat
    15061 tattgggcgg tcaacaccac ggggtcgctt ccgccgacgc cagcaaacgg cggcaaagtc
    15121 gtggcgatgg aaatggtgac cgcagcggtg tctagcctga cggcgggaac caactacttc
    15181 cacatcgtgc cgattgacgc gatgtcgaac gtgggcaccg tcgaaaacat cttcaaggtc
    15241 aacatcaact cgacgccgcc aacgctccgt tcgtcgagcc acccgtcgtc cggtacttgg
    15301 tacgcaagcg gcgatgtgtt cttcgagtgg accttcccgc aagacgacaa gaacttcaaa
    15361 ggcatctact acgtgctcga ccactacggc gagacgctgc cggcggcatc gatggcgacg
    15421 atggttccgt cgacgcagaa gcagcttctg cgttcgggtc tggccaacgg cgtgtgggtg
    15481 atgcacatgg tgccgattga tcagcgcggc tacctgacca agaccgccag tcactaccgc
    15541 gtgttcgttg gcaccgatcc gggcaagggc gggctgctcg gccaggttgt cgacgccatg
    15601 ggcaagccgg tgagcggcgc caaagtcacg gtcaaccgcg gcctgtggaa cgccaccacc
    15661 aacgcttcgg ggtcgtacaa cttcatggcg acgattccga cgggtacgtg ggaagttcgc
    15721 gcgatcagtc cagacgggtc gcttaccgaa accaagatgg acatggtcac caaagacggc
    15781 accaccaccg tcaatttcac cctcaagtag tctttccccc cgcgttttcg cccgttgtcc
    15841 tgcggcgggc gttttggcaa aaccgcttgt tgtccgcttc gccgctcgcc taatctatgg
    15901 ccgcatgaag ccccgtcgca cccgcagcac atcgtggatt tccatcggcg tggctgccat
    15961 tgcggtggcc tggctttctg gctgctgtag caacgtccgc tgcatcccgt gtcagccggc
    16021 gggaatctat ctgaccgtgc tcgatgactg gacccagatg gtgcagtccg cggcgaaagt
    16081 ttccatcgct ggcaagccgt gtcagcccgc atcagtcaaa ggcagtgcgg tctacatctg
    16141 ctcggtgccg gcgggaactt actccatcga catcgaacag cccggctata caccgacgca
    16201 ggtgatgttt gcgctagagg cagaagggga tggctgctgt ctgtgcgggc ccatcgcgta
    16261 tggatcggcg tcgatcgtcc cggttgagac gatgtgacgc cgcaagcagc gtgggttaga
    16321 gcgaaaacgt cgagcgccgc acaaagcgac cgtggccggg acggcttacg tgctggcctt
    16381 ggtcgatgac cagctcaccg cgggaaaaca cgaaacgcgg cgaaccgggc accgtcttgt
    16441 cgggataggc ggcgtagtcg acgcgcatgt gcaggttttc gtgcgatagc ttgagcgcac
    16501 gcttggggtc ccagacgacg acatcggcat cggaaccgac cgcaatcgat cccttctttg
    16561 gatacagtcc aaagattttg gctggcgcgg tgctggcgat ctcgacgaag cgattgagcg
    16621 acagcttgcc ctcggcgact gcctcgaaca aaaggtggag ccgcgtttcg atgccaggca
    16681 tgccattggg cactttggca aagctgtccc gtcccagcac tttctgatct ttcatgcaga
    16741 acgggcagtg atccgtcgac accacctgca agtcgtagtt gcgcaagccg cgccacaggt
    16801 gttcgtggtg actggccgga cgcagcggcg gtgtgcagac gtacttggct ccgtcaaacg
    16861 gatctgtcgg cgtgccgcgc agtgcgtcgt cggacagaaa caagtattgc ggacaggtct
    16921 cggcgtaggc cggcagaccg cgatcacgcg cttcacgcac ccgctcgagc gcccgcgccg
    16981 ccgacaagtg gacgatgtaa accgggactt tggccatttc ggccagcgcg atcgctcgct
    17041 cggtgcctgt cgcctcggcg acttcgggcc tcgtcagcgc gtgataaatc ggcgccgtgt
    17101 gccctttggc aaccgctcgc tcgacgagca catcgatcgg cagcccggtt tccgcatgca
    17161 tgcagataag cgctccgatt tcccccgcac gcagcatgca gcggaaaatc gactggtcgt
    17221 cgagcagcag cacgcccgga taggccataa acagcttaaa actggtcacg ccttccgaca
    17281 cgatgtcacc catttccgtc agcaccgact gcggcgcgtc ggttgcgatc atgtggaagc
    17341 cgtaatcgat acagcctttg cccgcggctt tcgcgtgcca ggtgtcgagc gcctgccgca
    17401 gcgtgccgcc tttttgctgg atcgcgaagt cgatgatggt cgtcgtgccg ccgtatgccg
    17461 ccgcgcgggt gccggtcaga aagtcgtcgc tcgacgtggt gccgccaaat ggcatgtcca
    17521 agtgggtgtg ggcatcgatg ccgccgggga tgacgtaaca gccgctcgca tcgatcactt
    17581 tgacgtcgct gcccgtcgga atcggctgac tcggatgggt gtgacttacg atcttctcac
    17641 cgtcgatcca gaggttcgct tgataggtgt cgctcgccgt gaccagggtt ccgttcttta
    17701 tgaccgtgac catgtttcct ccaggttcgc ggcaccgtag cgcagagccg ctcgacgtga
    17761 aaacccccaa aatcgcagcg cttgccggga ctttttacaa agccccctgt ccgccgctcg
    17821 ccaagatgct gtaatgaaga aatcgaggcg aaacagacca tgcggccttt gcggatcgga
    17881 ctttgcggac ttttgctgct ggcgtccccg cgcgcaatgt ccgcggtgcc ggtcggtctg
    17941 cgtagctcga cggtgaagcc agcgactcag tacatcaaca acctgccgcc gccaccccag
    18001 ccaccgccgc caccaccgtc ggtgatggat gggcaaaaag atcagctgat cgaacgcgat
    18061 gtcgtccgcc gcgtgatcta tctgcacatc aaacagatcc gcaactgcta ccagcaggca
    18121 ctgttcaaaa accccaagct cgccggtcgg ctggtcggtc aatggggtga gccgcgccaa
    18181 tgccttcctc gtcagtcgcg acgccgagga tcgcccggtc tatggcggaa cgcccgccga
    18241 cttcgcggtg gtgcaggcga tccgggagat gaaggcgcgc gggttgcgcg tcaccttcta
    18301 tcccttcct
//
LOCUS       DAORGN010000092        18144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1251_length_18144_cov_5.471281, whole genome shotgun sequence.
ACCESSION   DAORGN010000092 DAORGN010000000
VERSION     DAORGN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18144
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1251_length_18144_cov_5.471281"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKL17_11580"
     CDS             complement(<1..638)
                     /locus_tag="PKL17_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNK45420.1"
                     /translation="MNASGMPAGEGCQLISVSLRSLLSDEELGLLRAASDVGCALSLC
                     ELDGSAVVGDAPPQTVLDALQSGAPQLGTSDGREYLGTLLFHDRDALGYVVCSLPSAS
                     ESRARSLRAAHYLIRMSETLLSSAVRRTLMTRLHQLQVEEAYLELQLKTQRLEQAVAR
                     MKDSERIKASFLATVSHELRTPLTSVIGYTEMVIEGMAGPISDEQKEYLDTI"
     gene            complement(635..1225)
                     /locus_tag="PKL17_11585"
     CDS             complement(635..1225)
                     /locus_tag="PKL17_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNK45421.1"
                     /translation="MIVDSPTGTGDPTADLSAHQSSHFFDLLPIVLDGDGGRLKLRLI
                     AVPGHFMYEPTRRLLLRSADGVVFVADSNLPFEENHASFAELRSSLRENGRAPDSMPM
                     VVQWNKSDDAGSGTGLLAAQRLLHGRSDVAVIPACADRGVGVVETLLHLVRLVWPTAM
                     HERESLASLGLDLPLLLDLLRQRLQVRESGSEGALP"
     gene            complement(1329..3944)
                     /gene="lon"
                     /locus_tag="PKL17_11590"
     CDS             complement(1329..3944)
                     /gene="lon"
                     /locus_tag="PKL17_11590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNK45422.1"
                     /translation="MPNPLSRDPDLDQRRDRLGQPERLELPLLPTRDLVLFPFMQAPL
                     LVGRELSTQAVQQAENTDDKLIFVCLQRRSDDENPSRVDALWEVGTVATIVRSAKLQD
                     GRLKVWVQGLRRAQVVAVGAGQTALSAQVAIRPDDDEPATEPRLQLASHLLGTQAETA
                     ALQRQAREDVERLLREGKLRSQELLPLLDECDEAEPGRFADLLAASMSLPVSDAQALL
                     ELRSGRERLYKLAAQIRREIELHKLQEGIRAKTRDSLSRAQREHFLREQLRQIHSELD
                     GADDELGELRRQLQQALLPGDVQAEADLQLRRLASMPPHSAEGQVIRAHLEWLCELPW
                     QALTQDRLDLADVAQLLDEEHFGLQAVKDRLLEFVSVLKLRRQSVGRPRRLGTVLCLC
                     GPPGVGKTTLARSLARAIGRRFVRVALGGIRDEAEIRGHRRTYVGAMPGRLVAGIRQA
                     GARNPVMLLDELDKLCVDAHGDPSAALLEALDAEQNAAFRDHYLGVPIDLSEVLFIAT
                     ANNPEKIPDALRDRLEIIQLSGYSDEEKLGILDRHIIPRALSDTGLLPTYSVRFTDPA
                     LRALATEYTREAGVRDLTRQVETICRRLAKQIVEQEDRSEPQLPFDPQIAIHHRQAPQ
                     LPLKGVQASRGPSQLLVSRREVLRFLGAPPLPPLRSLPSGRVGAALGLAWTPVGGEIL
                     TVESQRLSGSGGLHLTGQLGEVMRESAQTALSFARAWLRDRGIESQPGEIHVHVPQGA
                     IPKDGPSAGVTVAVALTSLLSGIAVRQGVAMTGELTLHGRVLPVGGVEEKLLAARRAG
                     LKTVILPRDCEPAVRLLPQSLRRRLEIVLVADMDELLTFALLTLPTRAHTNEQGAPPE
                     SRAKSTTFRHGAAKS"
     gene            4210..4632
                     /locus_tag="PKL17_11595"
     CDS             4210..4632
                     /locus_tag="PKL17_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45423.1"
                     /translation="MQVGSSASLDGLCDRLLRSTAACKVLVCTIEGAVVAHAGERNIL
                     RGSLANEVADLAADLIIETQQASHLEIPVEDRYVTLSVPKDNPSPSAMQLGAAPLGHR
                     ALLLVLFPAHVDAGQVRVRVRRARPQMLRILSSPSLTS"
     gene            complement(4656..6920)
                     /locus_tag="PKL17_11600"
     CDS             complement(4656..6920)
                     /locus_tag="PKL17_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNK45424.1"
                     /translation="MRAERNSDGPRSLFSYRPYWAHRFGVAPFLPTSRAEMDELGWDS
                     CDIILVTGDAYIDHPSFGMALVGRLLEAHGFRVGILDQPDFGSAEAFRSLGPPNLFWG
                     VTGGNMDSMVNRYTSDRRIRSDDAYSPSGVAGLRPDRAVMVYTKRCREAFPGIPVVIG
                     GIEASLRRIAHYDYWSDKVRRSILIDSGADLLVFGNAERAIVEIAHRLGSGKDITQVR
                     DLRGTAFVQSTPADYVEIDSTHVDKPGRIDPRPDPYAMEEARAQAPCGSEGKPEGDSQ
                     SDSESAKSSSPLVQITPRKVDRSHQVIRMPSFEAVQKDPVLYAHASRILHREANPGNA
                     RALVQRHGRVDVWINPPPIPLSMQEMDALYELPFARTPHPRYSGQKIPAYEMIKFSVT
                     IMRGCFGGCSFCSITEHEGRVIQSRSETSILREIEQIRDRTPGFTGIISDLGGPTANM
                     YRMACKSREVEAACRLPSCVYPDICDNLGTDHGPLVRLYRKARAIPGVKRIFVASGVR
                     YDLAVRSPEWIRELAQHHTGGYLKIAPEHTEDGPLSHMMKPGIGTYDRFKELFDRYSQ
                     EAGKEQYLIPYFIAAHPGTTDEDMLNLALWLKKNGFRPDQVQAFLPSPMATATAMYHS
                     GKNPLSPLSFDKDVPVVKSLEQRRLHKAFLRYHDANNWPILRDALRRMGREDLIGNSK
                     HHLVPTWQPAGTGIAHEGRRGKHAAQPVPQPVNQAKGRNLPRPSHAAKPSRPPQKGMA
                     LTQHTGLPPVPHDR"
     gene            complement(6963..7712)
                     /locus_tag="PKL17_11605"
     CDS             complement(6963..7712)
                     /locus_tag="PKL17_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45425.1"
                     /translation="MNPRAHHPRFGRFFAGMSMLLWLGIPLGGLGTADARESTDVSAS
                     VAAAKVRPAKSASFRSQAHRLEPGMATPLHAKPLLGPFADKAAADVQGCESPTTLSKL
                     KTPYVSVETCAHKDSTALVIGTAHGLFLLPDFFARTASAVNRIGNVRIEQGRLLLESV
                     STEGRFASLSSSFLTVCADSASGLSCVGPLEVGRVFVGYEGGKEDGHRLPPQTRFEYR
                     FRFLPDDVLSLEPVVKPVSADVAGLHKLRFP"
     gene            7824..8447
                     /locus_tag="PKL17_11610"
     CDS             7824..8447
                     /locus_tag="PKL17_11610"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNK45426.1"
                     /translation="MPVTDIRAEFGEFVSPQWTRTQLCRLLRDYFRRTRPDTPVRVGT
                     DLPFYYSPSDRLARVVPDLYVMEGVEPDEQLRSYRVWERGRAPQLVVHLVDGAISAED
                     GLMMHFLRLGVQDVVLYDPLWFLQTDRNIKGRRLLWHYQRRPSGDGMRLLPQRHPARV
                     PLLAYGLWLCHRGGADLRLYVSGQEEVPPESALWLLPEERSASSRPT"
     gene            8485..10038
                     /gene="accC"
                     /locus_tag="PKL17_11615"
     CDS             8485..10038
                     /gene="accC"
                     /locus_tag="PKL17_11615"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNK45427.1"
                     /translation="MTTTQPNIRPIRKILIANRGEIAVRVIRACRELGIRSVAVYSDA
                     DRGALHVRMADEAYHIGPPPPRESYLDMDKVADTAVKAGADAVHPGYGFLSERAEFVE
                     ACEKRGLIFIGPPAQAMRQMGEKTRARIKMQAAGVPTVPGDNGIDGRGFPTVETALVA
                     AEKIGFPVMLKAAAGGGGRGMRLCESADKFPAAFESAKREAKAAFGDDTVYLEKAIIR
                     PRHVEIQVFGDRHGNHVYLFERDCSVQRRNQKVIEESPCPVLDEDLRRRMGEVAARAA
                     AAVDYVGAGTCEFLLSEDRNFYFLEMNTRLQVEHPVTELVTGLDLVHLQIRVAEGHPL
                     PFRQQDLMLRGAAIECRVCAEDSLRFLPSPGKITRLRNPSGPWVRNDSGVYQDAEISM
                     FYDNLISKLIVWAPTRAEAIERMSRALSEYQIGGIKTNLGFHRRVMQEPDFRAGRYNT
                     GYIEAHKQTLLSAQPLDDQTVRTAAIAAAIHASSSSTASAVKHETAGGTEISAWRAGW
                     PAHGLRDGK"
     gene            complement(10263..10532)
                     /locus_tag="PKL17_11620"
                     /pseudo
     CDS             complement(10263..10532)
                     /locus_tag="PKL17_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            10913..12595
                     /locus_tag="PKL17_11625"
     CDS             10913..12595
                     /locus_tag="PKL17_11625"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNK45428.1"
                     /translation="MTHTAQVRPLYEIAQDLGLGQDDLIPYGQDKAKLAPRFFSRTNP
                     QSGRLILVSAINPTPAGEGKTTTAIGLQMGLQRIGKKACLALREPSLGPVFGVKGGGT
                     GGGRASLHPADDINLHFTGDLHAITSANNLLAALADNAVYHRQRFAEKGELDGRQISW
                     GRAMDVNDRFLRRTIVGLGGKAHGTPREARFDITAASEVMAVLALSTGYDDLKARLGR
                     IVVGATESGQPVTADDLQATGAMVALLRDALLPNLVQTMEGGPALVHCGPFANIAHGC
                     SSVLATKFGLSLSDYLITEAGFGFDLGGEKFLDLKCRQLGVWPRLVVLVATLRALKLH
                     GGAKLATAGAANSAALANGMEHLRKHLESLAQFGLPAVVAINVFPDDSEEELNAVEQS
                     VAQMGAKAARSTAFAHGGDGATALAELVASEAEKTDASPPQARYLYELSDPLLDKLTK
                     IARTVYGADGVVLSQTASKDWERFSAWGYGKLPVCVAKTPLSLSDDPARVGRPKGHTI
                     TVRELRLSAGAGFLVALTGEIVTMPGLPAKPAAWNIGLTESGRIRGLMQNDS"
     gene            12645..14474
                     /locus_tag="PKL17_11630"
     CDS             12645..14474
                     /locus_tag="PKL17_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNK45429.1"
                     /translation="MNATSHYRSNLRDLFFNLFEYHDIGANILGQGPFQNLDVPMVKE
                     MLIQLEKLAVNELAASFASADREGVELGSDGNVRLPKSLRASLDAYHDRDYHMLEMPE
                     HLGGMGAPPSLNWAGYELLAGSNSTAGFYLTTQFMLRIIDRLGTAEQKARFLPAALER
                     RWNCTMVLTEPDAGSDVGAGKTRARHVEGDEWHIEGVKRFITGGDNDYFENIIHLVLA
                     RPEGAGPGTKGLSLFLVPKFWVEKDGSMGARNGVRTTKVEHKMGIKASATCELVFGDE
                     TPARGLLLGNVHDGIRQMFQVIEQARMAIGVKSMSTLSTGYLNALGFAQERIQGPDLL
                     RAMDKSSPRVEILAHPDVRRMLLLQKCHAEGMRALCFYAASLQDQRELHAATDHKKSE
                     ALERRNDLLLPLVKGYCSEKAYEMLSLSLQCFGGSGYLQDYPIEQYIRDQKIDTLYEG
                     TTHIQALDLLLRKVARDGGQLLRELLAEAQATIASEEGGADLADARAALGKALENLQG
                     MLMALLGKMGESLYHVGLQGNRVLMALAEVLIAWLLIRHGALAVRKLAELPEGAESER
                     GFYQGKIFSARYFATQVLPRLSFDRRQVEASDLAPMNLPVSAF"
     gene            complement(14532..16463)
                     /locus_tag="PKL17_11635"
     CDS             complement(14532..16463)
                     /locus_tag="PKL17_11635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNK45430.1"
                     /translation="MSPQNQTPPRYTEEDIVVLEGLEPVRKRPGMYIGGVDSRGYHHL
                     LWEILDNAIDEVINGHATTIKVILHSDRRSVTVDDNGRGFPTGILPKYGKSAIEVILT
                     TLHSGGKFQQGSYTHSGGLHGVGASVVNALSVELWVRVRRDGKEVEQGYARGIPVTPL
                     RSVGAAKGSGSKVYFRPDPEIFGEKASFDLELVRERLEAKSYLHKGLRIVFTDEASGE
                     TVELSHKGGIADYLQKWATERGKPVLCPPVYFFRDADEGTTRFELSFLWTEGTDESIK
                     SFVNGIPTPSGGTHENGLKAGINKGVRNFVDTHGLLPKGLQLTAEDIREGLLAILSCY
                     IHEPQFQGQTKERLNNPEVTAEVDGAVRTFVENWLNSNKQTAEAIVSRIVAAARAREA
                     MKAASQAVPRKSVVGQRLNLPGKLADCQSTDPGQAELFIVEGDSAGGSAKQGRNRKNQ
                     AILPLRGKVLNAEQASLSKLLSNRELQDIVAALGCGMGDKYDEGRLRYGRIFLLMDAD
                     SDGHHISTLLLTFIYRYIPKLIENGHVFIAQPPLYRVDIGKETHWVLDDKQKDELLAK
                     QKPNAKVEIQRFKGLGEMMPQQLWATTLDPKRRVALRVEIQDREQTDRVLGDLMGKDP
                     SARFRFIMEHAGEAGALDV"
     gene            16555..17388
                     /locus_tag="PKL17_11640"
     CDS             16555..17388
                     /locus_tag="PKL17_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNK45431.1"
                     /translation="MNPPSLDALEHAKRTLIDVGIHYGPRLVVAMLILIFGGFVAHRA
                     GIISRRWFSKLELDQPLGQLLGRALTVLVFLLFLTLALQNLGVELLPLFASLGVAGVG
                     VGLAAQGVLGNLVAGLTIIFTRPFRIGEWVQMIGVEGQVESIDLFSTTLSHPDRSLVV
                     IPNRKIVGEVLHNYGKLRQLDLLIGVAYSTDIQKALMVVRDVLTQNDRVTKDIAPIVG
                     VTLLGEKSVGIAIKPWVAVTDYVPAVGELNQALVEAFRTHRIEISFPQRDVRIVGSVV
                     A"
     gene            complement(17399..>18144)
                     /locus_tag="PKL17_11645"
     CDS             complement(17399..>18144)
                     /locus_tag="PKL17_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45432.1"
                     /translation="RPRGGRDAPRNDGGPVSMYETVSRDLAQSAVGKRGGAWRRVVRV
                     LCATACVAMLSQSGCDNSNKPIIIKGPSVQPVQDIRWGTMRAVHRVGIDAAKGDGREQ
                     VTVRGMIAVERPDRFRLKAIGPGGITLFDLIKVGGDVKVVTSMAGADSSLQQKVLLSI
                     GADLSAMFDLEPLHPSHKKYVDLKVGELRVVENERTIRCQQFKEVQGRSVPTHVEIDN
                     TALNYKVSIDVEEASLDEKLDPALFRMNK"
BASE COUNT         3629 a         5568 c         5390 g         3557 t
ORIGIN      
        1 aaaatcgtat cgaggtattc cttttgttcg tcgctgatcg gcccggccat accttctatc
       61 accatctcgg tatagccaat caccgacgtc agcggcgtgc gcagctcgtg cgagaccgtc
      121 gccaaaaacg acgctttgat tcgctccgag tctttcatcc gcgccaccgc ttgttccagg
      181 cgctgcgtct tgagctgaag ttccaggtac gcttcttcga cttgcagctg gtgcagccgc
      241 gtcatcagcg tccgccgtac cgccgacgaa agaagcgtct cgctcatccg aatcaagtag
      301 tgcgccgcac gcagcgaccg agcccgtgat tcacttgcag aaggcagcga acaaacgacg
      361 taaccgagcg catccctgtc gtgaaacagc agtgttccca ggtattcccg tccatcgctt
      421 gtgccaagct gcggggctcc tgactgcagc gcgtcgagca cggtctgcgg cggcgcatcc
      481 ccgaccacag ccgatccgtc gagctcgcaa agcgacagcg cacagccgac atccgatgcg
      541 gcacgcaata gcccgagttc ttcgtcggaa agcagcgatc gcagcgacac actgatgagc
      601 tgacagccct cgcctgccgg catgccactt gcgttcatgg cagcgctcct tccgatcccg
      661 attcgcgcac ttgcagacgc tgacgcaaca gatcgagaag cagcggtaga tcgagtccca
      721 aagacgccag cgactcccgt tcatgcatcg cggtcggcca caccaagcgc acaagatgaa
      781 gcagcgtttc gacgacaccc acaccgcgat cggcacaggc tgggatcacc gcgacatcgc
      841 ttcgtccgtg cagaagccgc tgcgccgcca aaagcccggt ccccgaccct gcgtcgtcgg
      901 acttgttcca ctgcaccacc atgggcatcg agtcgggcgc acggccattt tcccgcaggc
      961 tgctccgcag ctcggcgaag ctcgcgtggt tttcttcaaa cggcaggttg ctgtccgcaa
     1021 cgaaaacgac cccatccgcg ctccgcaaaa gcagccgccg cgtcggttcg tacataaagt
     1081 ggcccggcac cgcaatcaac cgcagcttga gcctgccgcc gtcaccgtcg agcacgatcg
     1141 gaagcagatc gaaaaaatgg ctgctctgat gagccgaaag gtccgcggtc ggatcacctg
     1201 ttcccgtcgg gctgtctacg atcatcaaac ggctgcgaaa gcgatcaagc agccgggccg
     1261 caagacgtcg caggttggtg gtctttcccg atccgagagg cccacagtag acgatctgga
     1321 ctgtatggtt acgatttggc ggcgccatgg cggaacgtcg tggacttcgc tcgggattcg
     1381 ggcggagcac cctgttcatt agtatgtgct ctggtcggca aggtcaaaag ggcgaacgta
     1441 agcagttcgt ccatgtccgc gaccaaaaca atttcaagcc gcctgcgcag ggactgcggc
     1501 agcaggcgaa ccgccggttc acagtcacgc ggcaggatca cggtttttag cccggcgcgt
     1561 cgcgcagcca gcagtttttc ttcgactcca ccgacgggaa ggacgcgacc atgcagcgtc
     1621 agttcgccgg tcatcgccac gccttgtcgc accgcaatgc ccgacaaaag gctcgtcagt
     1681 gcgactgcta cggtgacacc tgccgacgga ccgtctttgg gaatcgcacc ttgcggaacg
     1741 tggacgtgaa tttcaccggg ctgactctcg atgccgcgat cacgcagcca cgcccgcgca
     1801 aagcttagtg ccgtctgagc cgactcccgc atcacttccc cgagctggcc ggtcagatgc
     1861 agaccccccg agcctgatag ccgctgcgat tcgacggtaa gaatttcacc accgacggga
     1921 gtccacgcca gacccagtgc cgccccgacc cgacccgatg gcagcgatcg cagaggtggc
     1981 agaggcggtg cacccaaaaa gcgcagcact tcgcgtcgtg aaaccaagag ctgggatggc
     2041 ccgcgcgacg cctggacgcc tttgagcgga agctgcggcg cctgccgatg atgaatcgcg
     2101 atctgtggat caaacggcaa ctgcggctcg ctgcgatctt cctgttcgac aatctgcttg
     2161 gccagccgac gacagatcgt ttcgacttgt cgggtcagat cgcgaacccc agcttcgcgg
     2221 gtgtattccg ttgccaaagc ccgcaacgcc ggatcggtaa agcgcaccga ataggtgggc
     2281 aaaagacccg tgtccgaaag cgcccgcgga atgatgtggc ggtccaaaat gccaagcttt
     2341 tcttcgtcgc tgtagcccga tagctggatg atttcgagcc ggtcacgcag cgcatcggga
     2401 attttttccg ggttgttggc cgttgcgatg aacagcactt ctgacagatc gatcggtaca
     2461 cccaagtaat gatcccgaaa cgctgcgttt tgttcggcat ccagcgcttc gagcagggcc
     2521 gccgacggat cgccatgggc gtcgacgcac agcttgtcca gctcgtcgag cagcatcacc
     2581 ggattgcgcg cgccggcttg gcgaatccca gcgaccagac gacccggcat cgcccccaca
     2641 tacgtccgtc gatgaccgcg aatttccgct tcgtcgcgaa tccccccgag cgccacgcga
     2701 acaaaacgtc gccctatcgc ccgcgccaaa gacctggcca gcgtcgtttt gccaacgcca
     2761 ggcggaccgc acagacacag caccgtcccc agccgtcgcg gtcgcccgac cgactgacgc
     2821 cgcagcttca gcacactcac gaactcgagc aggcgatctt tgacggcttg cagaccaaag
     2881 tgttcctcgt cgagaagctg cgccacatcg gccaaatcga gccggtcctg ggtaagcgcc
     2941 tgccacggca gctcgcacag ccattccagg tgagcccgaa tcacttgtcc ttcggcactg
     3001 tgcggcggca tcgacgccag acggcgcaac tgcagatcgg cttcggcttg gacatccccc
     3061 ggtagcagcg cctgctgaag ctgccttcgc agctcgccca gctcgtcgtc cgcgccatcc
     3121 agttccgagt gaatctggcg aagctgctcg cgcagaaagt gttcgcgctg cgcccggctc
     3181 agcgaatcgc gggtcttggc ccgaatgcct tcttgcagct tgtgtagctc gatttcgcgc
     3241 cggatctgcg ccgccagctt gtagagccgc tcgcgcccac ttcgcagctc gagcagcgct
     3301 tgcgcgtcgg acaccggcag gctcatgctc gctgcgagca gatcggcaaa acgccccggc
     3361 tcggcttcat cgcactcatc aagcagtggc aagagctcct ggctgcgcag cttgccttcc
     3421 cgcaagagcc gttcgacatc ttcgcgcgcc tgcctttgca aagccgccgt ttccgcctgc
     3481 gtcccgagca gatgactggc aagctggagc cgcggctctg tcgccggttc atcgtcgtcg
     3541 ggacggatcg ccacttgcgc cgacagcgcc gtctgccctg cccccaccgc gacaacctga
     3601 gcacgacgca gcccttgcac ccagactttc agccgaccat cttgcagctt ggcgcttcgc
     3661 acaatcgtcg cgacggtgcc cacttcccac agcgcgtcca ctcggctcgg attttcatca
     3721 tcggaacggc gctgcagaca gacaaaaata agcttgtcgt cggtattttc ggcctgctgc
     3781 accgcctgcg tcgacagctc gcgcccaacg agaagcggcg cctgcataaa cggaaacagc
     3841 accagatcgc gcgtcggcag aagcggcagt tccagtcgtt cgggctggcc caaacggtca
     3901 cggcgctggt cgagatcagg atcacgagag agcggattgg gcatcaaggc tccgcatgcg
     3961 acaggggtaa aagtcgagtc aagtcgcaaa tctgcctacg atggtaatca gggcggctcg
     4021 cgtcgcaaca tctgacgccg attttcgccc gttggccgat gcttgctggg aatttcccgt
     4081 cggaaacagc gaaaatgggt cgcgcctaaa aacgcacact caactgctca ataaaccccc
     4141 gcgtcgcaag tgcagggtgc ctgtctaaag ggctagcaag cgggctgtaa gatgtgcgaa
     4201 aatgatctca tgcaagtcgg ttcgtccgca tcgctggacg gtctctgtga ccggcttctg
     4261 cgctctaccg cggcgtgcaa ggtattggtc tgcacgatcg agggcgcagt agttgcacac
     4321 gctggcgagc gaaacatttt gcgcggttcg ctcgccaatg aagtcgccga tttggcggcg
     4381 gacctgatca ttgaaaccca gcaagccagc catctcgaaa ttcccgtcga ggaccgctac
     4441 gttacgttat cggttcccaa ggacaatccg tcgccttcag cgatgcagct tggtgccgcg
     4501 ccgctcggtc atcgggcgct gcttttggtc ctgtttcccg cccacgtcga tgctggacag
     4561 gtccgcgtgc gcgtgcgcag agcccgtccg cagatgctgc gaatcctgag cagtccatcg
     4621 ctgaccagct aacctgttcg ccacagccga tttttttagc gatcgtgcgg aaccggcggc
     4681 agaccggtgt gctgcgtaag cgccattcct ttttggggcg gacgacttgg tttcgccgcg
     4741 tgggaaggcc gcggcagatt ccttcctttg gcttggttca cgggctgtgg cactggctgc
     4801 gcagcgtgct tgccacgacg gccttcgtgc gcgattccgg ttcccgccgg ctgccaggtc
     4861 gggacaagat gatgcttgct gtttccgatc aaatcttcgc gtcccatccg gcgcagtgcg
     4921 tcgcgcaaaa tcggccagtt gttggcatcg tgatagcgca gaaacgcttt gtgcagacgc
     4981 ctctgttcca ggctttttac caccggaacg tctttgtcaa aggacagcgg actgagcgga
     5041 ttttttccgc tgtgatacat cgccgttgcc gtcgccatcg gtgaaggaag aaacgcctgc
     5101 acttgatctg gacgaaagcc attttttttc agccacagcg ccaagttcag catgtcttca
     5161 tctgtcgttc cggggtgcgc tgcgatgaag tacggaatca ggtactgctc ttttccggct
     5221 tcttgggaat acctatcaaa cagttccttg aatcgatcgt atgtaccgat tcctggcttc
     5281 atcatgtggg acagcggtcc gtcttcggtg tgctcaggag cgattttcag atagccgcct
     5341 gtgtggtgct gcgccagctc tcttatccat tccggagacc gcaccgccag atcataacgg
     5401 actccggatg cgacgaagat ccgcttcaca cccggaatcg cccgcgcttt gcgatacagc
     5461 cgaaccagcg gtccgtgatc ggttcccaag ttgtcacaga tgtccggata cacacacgaa
     5521 gggagtctgc acgcagcctc tacttctcgg cttttgcagg ccatccgata catatttgcc
     5581 gtcggaccac cgagatcgct gatgattccg gtaaaccccg gcgtgcgatc gcgaatctgt
     5641 tcaatttcgc gcagaatcga cgtttccgaa cggctctgga tgactcttcc ttcatgttcg
     5701 gtgatcgaac agaaactgca gccgccaaaa cagccgcgca tgatcgtgac cgaaaatttg
     5761 atcatttcat acgcaggaat tttctggccg ctgtagcgag gatgcggagt ccgcgcaaac
     5821 ggcagctcgt acagtgcatc catctcttgc attgaaagcg gaatcggcgg cggattgatc
     5881 cagacatcga cgcgaccatg tcgctgcaca agtgcgcgcg cattcccagg gttggcttcg
     5941 cgatgcaaga ttcgagatgc gtgcgcatac agcaccggat ctttttgcac cgcttcaaag
     6001 cttggcatcc gaatcacttg atgggagcga tcgactttgc gcggcgtaat ttgcacgagc
     6061 ggcgacgatg atttcgccga ttccgaatcc gattgggaat ccccttccgg cttcccttcc
     6121 gatccacaag gagcctgcgc gcgcgcttct tccattgcat agggatcggg ccgcggatcg
     6181 atccgtcctg gcttgtcgac atgcgtggaa tcgatctcga cataatccgc cggcgtcgac
     6241 tgcacaaacg ccgttccgcg cagatcccga acctgggtga tgtcctttcc ggaccccaaa
     6301 cgatgcgcga tctcgacgat ggcgcgctcg gcattcccaa acaccagcag atcggcgcct
     6361 gaatcaatca ggatcgatcg gcgaactttg tctgaccagt agtcgtagtg cgcaatgcgg
     6421 cgaagactcg cttcgatgcc gccaatcacc accggaatgc ccggaaaggc ttcgcgacag
     6481 cgcttggtat acaccatcac cgcgcgatca ggacgcagcc cagcgacgcc agaaggagag
     6541 tacgcatcat cggaacggat gcggcgatcg ctcgtgtagc gattcaccat cgagtccatg
     6601 ttgccgccgg tgacgcccca aaacagattc ggtggaccga gcgaacggaa ggcttcggca
     6661 ctgccaaagt ccggctgatc caaaatgccc acccgaaagc cgtgcgcttc gagcagccga
     6721 ccgaccagcg ccatgccgaa gcttgggtga tcgatgtatg catcgcccgt caccaaaatg
     6781 atgtcgcagc tatcccaccc gagttcgtcc atttccgcgc gtgaagtcgg cagaaaagga
     6841 gccacaccga accgatgcgc ccaatagggg cgataggaaa acagggatcg gggtccgtcg
     6901 gaatttctct cggcgcgcat gccgccccgc gtaccacgca gaggccccga aggtcaaacg
     6961 ccttatggga aacgcagctt gtgcagtccg gccacgtccg cagagacagg tttgacgacg
     7021 ggttccagcg aaagcacatc gtccggaagg aagcggaaac ggtactcaaa gcgggtctgc
     7081 ggcggaagtc tgtgcccgtc ttctttgccg ccttcatacc ccacaaagac ccgccctact
     7141 tcgagtggcc cgacgcaaga aagaccgctc gcggaatcag cacacaccgt cagaaaggaa
     7201 gaagagaggg aggcaaaacg accttctgtg gaaaccgatt cgagaagcaa tcgaccctgt
     7261 tcgatccgca cattcccaat gcggttcaca gcgctcgcgg ttcgcgcaaa aaaatcgggc
     7321 aacagaaaca gtccgtgtgc cgttccaatg acaagcgccg tgctgtcttt gtgcgcgcaa
     7381 gtttcgacgg aaacataggg tgtcttgagc ttgctcagag tcgttgggga ttcacagcct
     7441 tgcacatccg ctgccgcttt gtcggcaaac ggacccagaa ggggcttggc gtgtagcggt
     7501 gtcgccatcc caggttcaag acgatgagcc tgcgatcgaa acgaggccga cttggccgga
     7561 cggaccttgg ccgcagcgac ggaggctgac acgtcggttg attcgcgagc gtccgccgtg
     7621 cccaaacccc caagcggaat ccccagccaa agcagcatcg acattcccgc aaaaaatcga
     7681 ccaaaacgcg gatggtgggc tcgcggattc atggcggcaa catagcgcag tggaccgagc
     7741 gcctgaaaag gaaactctgc gcgctacctt gcggcttgcc ctgcaaacag ggttcgctac
     7801 gatggggagg gagaaacgcc agcgtgccag taaccgacat ccgagcggag tttggtgagt
     7861 ttgtctcgcc gcagtggacg cgcacccagc tttgtcgact gctccgtgat tactttcgcc
     7921 gaacccgccc ggatacgccg gtccgcgtcg gaaccgacct tcccttttac tacagcccaa
     7981 gtgatcgact ggcgcgggtt gtgcccgatt tgtatgtgat ggaaggcgtc gaacccgatg
     8041 aacagctgcg atcgtatcgg gtgtgggaac gcggacgcgc gccgcagctt gtggtgcatt
     8101 tggtcgacgg agcgatatcg gcggaagacg ggctgatgat gcactttctg cggctcggtg
     8161 tgcaggacgt ggtgctctac gatccgctgt ggtttttgca gaccgaccgc aacatcaagg
     8221 gacgacggct tctgtggcac taccagcgac gaccgagcgg cgatggaatg cgtcttttgc
     8281 cgcaaagaca tccagcgcgg gtgcctcttt tggcttatgg gctgtggctt tgtcatcgag
     8341 gcggtgccga tctgcggctg tatgtaagcg gtcaggaaga agtgccgcct gaatcggcgc
     8401 tgtggctttt gcccgaagag cgcagtgcca gcagtcgacc gacttgacaa agcgaagcca
     8461 aggcggggtt aatcgtcgct caagatgacg acgacccaac cgaacattag gccgattcgc
     8521 aaaatcctca tcgccaaccg cggcgaaatt gcggtgcgcg tcattcgggc gtgccgcgag
     8581 ctgggcatcc gatctgtcgc ggtgtattcc gatgccgatc gcggtgcgct gcatgtccgc
     8641 atggccgacg aggcttatca catcggtccg ccgccgcccc gtgaaagcta cctcgacatg
     8701 gacaaggtcg ccgacaccgc ggtcaaagcg ggcgccgatg ctgttcatcc cggctatggt
     8761 tttttgtccg agcgcgccga gtttgtcgaa gcgtgtgaaa aacgcgggct gattttcatc
     8821 ggtccaccag cgcaagcgat gcgtcaaatg ggcgaaaaaa cccgtgcccg catcaaaatg
     8881 caggccgctg gcgtcccgac ggtgcccggt gacaacggca tcgacggacg cggatttccg
     8941 acggtagaga ccgcgcttgt cgctgccgaa aagattggtt tcccggtgat gctcaaagct
     9001 gccgcaggtg gtggtggtcg cggcatgcgg ctgtgcgaaa gcgccgacaa gttcccagcg
     9061 gcgtttgaga gtgccaagcg cgaagccaaa gcggcgtttg gcgacgacac cgtctacctg
     9121 gaaaaagcaa tcatccgacc ccgccacgtc gaaattcagg tgtttggcga tcggcatggc
     9181 aaccatgtct atctgttcga gcgcgactgc tcggttcagc gtcgcaacca gaaagtcatc
     9241 gaggagtcac cctgcccggt tctcgatgag gatctgcgcc gccgaatggg tgaagtcgcg
     9301 gcccgcgctg ctgcggctgt cgattacgtc ggagccggca cctgcgagtt tctgctgtcc
     9361 gaggatcgga acttctactt tctggaaatg aacacccgtc tccaagtcga gcacccggtg
     9421 accgaactgg tcacgggact cgatcttgtg cacctgcaaa ttcgcgtggc cgaaggacat
     9481 ccactgccgt tccgtcagca agacctgatg ctgcgcggcg ccgcgatcga atgtcgcgtc
     9541 tgtgccgaag actcgctgcg ctttttgccg tcgccgggca agatcacccg tctgcgcaat
     9601 ccgtcgggtc cgtgggtccg aaacgacagc ggcgtgtacc aagacgccga gatcagcatg
     9661 ttctatgaca acttgatctc gaagctgatc gtctgggcac cgacgcgggc cgaagcgatc
     9721 gagcgaatga gccgcgcgct ttcggagtac caaattggcg gcatcaagac caacctggga
     9781 ttccaccgtc gggtgatgca ggaaccggac ttccgcgcgg gtcgctacaa caccggctac
     9841 atcgaagcgc acaaacagac gctgctttca gcgcagccgc tggatgatca gacggtgcgg
     9901 accgcggcga ttgccgcagc gattcatgcg tcttcatcgt cgacggcatc ggctgtgaag
     9961 cacgaaacgg caggaggtac cgaaatctcg gcgtggcgag ccggttggcc tgcgcacggt
    10021 ctgcgagacg gcaagtaacg atcgagcagc gacgtctagc gcttgccgcc ggactttttc
    10081 ttgccgcccg tcggtgcagc gggagctggc gtcggcgcgg cggctggcgt cgaggccgga
    10141 gccgcggcaa ccggggtcga cggagctggc gtcggagcag cgacgggtgc cggagcagca
    10201 acgggtgccg gagccggcgc ggccttggcg ggcctcgtcg atcggactgt cgctccgccg
    10261 gcatttacgc gctctttgag ctctttgccg accttgaaga acggcagccg cttgggattg
    10321 acaccgacgg gatcgccggt tcgcgggttg cgcccttcat aggctttata gcgccggatc
    10381 tcgaagctgc cgaagccgcg aatctcgatc cgattgcctt gtttgagcga ctcctccatg
    10441 gagtcgaaaa tacaattgat gatcagctcc gccttccctt tgggcaggtt gagctgggca
    10501 gctaccttct cgataagatc cgacttggtc atacctactc gcctctcgct acgcgtggca
    10561 tagacagagt caattcatcg taagcgaatt tacaaataga gatcaacagc ttttctacaa
    10621 aatacaggta ccctcggagg ggggctcgcg gacgatccaa ccggaagtat gctccgtcga
    10681 gaaaatgaac ccaatcggac attgtgaaga cagctaccca cgcccctcag agtgcccgcc
    10741 caatccattg gccaataata aacaaaatag caatataatg ctcaatttat tgaccattac
    10801 gaataaactt cgatcgcaaa caaagtggca acgtagtgca caatctgcca gtcgctctgg
    10861 atgtacgaca attgagcagt gagactcccc accacaaaga agagacaccg ccatgactca
    10921 caccgcacaa gtgcgtcctc tttatgaaat tgcccaggat ctggggcttg gacaggacga
    10981 cctgattccc tacgggcagg acaaggccaa gctggcaccg cggttttttt cgcggacgaa
    11041 tccacaatcg ggacgactga ttctcgtttc ggcgataaac ccaacgcccg cgggggaagg
    11101 aaaaaccacg accgcgatcg gtctgcaaat gggacttcag cggatcggca aaaaggcgtg
    11161 cctggccctg cgtgagccgt cgctcgggcc ggtgtttggg gtcaaaggtg gcggtactgg
    11221 cgggggtcgg gcatcgctgc atcccgccga cgacatcaac ctgcacttta cgggcgatct
    11281 gcatgccatc accagcgcca acaacctgct cgcggcgctg gctgacaacg cggtctatca
    11341 ccgtcagcga ttcgctgaaa aaggcgagct cgatgggcgg cagatttcct ggggccgggc
    11401 gatggacgta aacgatcgct ttctgcgtcg cacaattgtt ggtttgggcg gcaaagcgca
    11461 cggaaccccg cgcgaagccc gattcgacat caccgccgcg agcgaagtga tggcggtgct
    11521 ggcgttgtcg acgggatatg atgatctaaa agcacggctg ggccgaattg tcgtgggcgc
    11581 gacggaaagc ggccagcccg tgacggcaga tgatctgcaa gcgactggcg cgatggtggc
    11641 gctcttgcgc gacgcgctct tgccgaacct ggtgcagacg atggaaggcg ggccggcgct
    11701 tgtgcactgt ggtccgtttg ccaacatcgc gcacggctgt agttcggtgc tggcgacgaa
    11761 gtttggactc agtcttagcg actatctgat caccgaagcg ggttttggct tcgatctggg
    11821 cggggaaaaa tttctcgacc tcaagtgtcg acagcttggg gtgtggcctc gacttgtggt
    11881 gctggtagcg acgctgcggg cgctgaaact gcacggcggg gccaagctcg caacggcagg
    11941 ggcggccaat tcggcggcac tggccaacgg catggaacac cttcgcaaac acctcgaaag
    12001 tctggcgcag tttggtctgc cagcggtcgt cgcgatcaac gtgtttccag acgatagcga
    12061 ggaagagctg aatgccgtcg agcagtcggt cgcacaaatg ggcgccaagg ccgcacgatc
    12121 gaccgcattt gcgcacggtg gcgacggagc gactgcgctg gccgagctgg tcgcttctga
    12181 agcggaaaag accgacgcaa gtcccccgca ggctcgctat ctctatgaac tgtccgaccc
    12241 ccttttggac aagctgacga agatcgcacg cacggtatat ggcgcagacg gtgttgtgct
    12301 gtcgcaaacg gcatccaaag actgggagcg tttttccgca tggggatacg gcaagctgcc
    12361 cgtctgtgtc gccaagaccc cgctttcttt gtccgacgat ccggcccgcg tgggaaggcc
    12421 caagggccac acgatcaccg tgcgcgagct gcgactctcg gcaggggccg ggtttttggt
    12481 ggcattgacc ggcgaaattg tgacgatgcc gggcctgccc gccaagcccg cggcttggaa
    12541 catcgggctc accgagagcg gtcgcatccg cggactgatg caaaacgaca gctaagcaag
    12601 tagactgtgc cgcgtttttt cgcacctacg gcaaggagat cgacgtgaac gccacaagcc
    12661 actatcgcag caacctgcgt gacctgttct tcaacttgtt cgaataccac gacatcggtg
    12721 caaacatcct gggccaaggc ccgttccaaa acctggatgt cccgatggta aaagagatgc
    12781 tgattcagct cgaaaagctg gccgtaaacg aacttgcggc aagctttgca tctgccgatc
    12841 gcgaaggcgt cgagctgggc agcgacggca atgtgcgact gcccaagagc ctgcgcgcct
    12901 cgctcgatgc gtatcacgat cgtgactacc acatgctcga gatgcctgag cacctgggcg
    12961 gaatgggtgc ccccccgtcg ctcaactggg ccggctacga gctgctcgcc ggcagcaact
    13021 cgaccgccgg tttctatctg acgacgcagt ttatgctgcg gatcatcgat cggctgggga
    13081 cggcggaaca aaaagctcgc tttctgcccg cggcgctcga gcggcgatgg aactgcacga
    13141 tggttctgac cgaaccggac gccggtagcg acgtcggtgc cggcaagacc cgcgcccgtc
    13201 acgtcgaagg cgatgagtgg cacatcgaag gcgtcaaacg gtttatcacc ggcggcgaca
    13261 acgactactt cgaaaacatc atccacttgg tactcgcccg tcccgaggga gccggccccg
    13321 gcaccaaagg tctgtcgctg tttttggtcc cgaagttctg ggtcgaaaaa gacggctcga
    13381 tgggcgcacg caacggtgtc cgcaccacca aggtcgaaca caagatgggc atcaaagcat
    13441 cggcaacctg tgagctggtg tttggcgacg aaaccccggc gcgcggactt ttgcttggca
    13501 acgtccacga cggcattcgc cagatgttcc aagtcatcga gcaggcccgg atggcgatcg
    13561 gcgtcaagtc aatgtccacg ttgtcgacgg gatatctgaa tgcgctcggg tttgcgcaag
    13621 aacgaattca gggtccggac ctcttgcggg cgatggacaa gtcgtcgccg cgcgtcgaaa
    13681 tattggccca ccccgatgtg cgccggatgc tgcttttgca aaagtgccac gccgagggaa
    13741 tgcgcgcgct gtgcttctac gccgcgtcgc tgcaagatca gcgcgagctg cacgcggcga
    13801 cagaccacaa aaaatccgaa gcgctcgagc ggcgcaatga tctgctgctg ccgctggtga
    13861 agggttactg ctcggaaaaa gcctacgaga tgctgtcttt gtcgctgcag tgcttcggcg
    13921 gatcggggta cttgcaggac tatccgatcg agcagtacat ccgcgaccaa aagatcgaca
    13981 cgctgtacga aggaacgacg catatccagg cgctcgatct gctgctgcgc aaagtcgcac
    14041 gcgatggcgg acagctgctg cgagagctgc tggccgaagc gcaagcgacg attgccagcg
    14101 aggaaggcgg ggccgatctc gccgatgcca gggcagccct tggcaaagcg ctcgaaaacc
    14161 tccagggcat gctgatggca ctgctcggca aaatgggcga gtcgctttac cacgtcggtc
    14221 tgcaaggcaa ccgcgtgctg atggcgctgg ccgaagtgct gattgcgtgg cttttgatcc
    14281 gccacggtgc cctcgccgtc cgcaagctcg ccgaacttcc cgagggagcc gagagcgaac
    14341 gcggctttta ccagggcaag attttttcgg cgcgctattt cgcaacgcaa gtgctgccgc
    14401 ggctgtcatt cgatcgccgc caagtggaag ccagcgacct tgcaccgatg aacctgcccg
    14461 tcagcgcgtt ttagcccccg ccctgcccgc tgccgtcgcg acgctagacc gcgcgacgca
    14521 gcgaattccc actacacgtc gagagcgccc gcctcgcccg catgctccat gatgaaacgg
    14581 aagcgagcgg agggatcttt gcccatcagg tcgcccaaga cgcgatcggt ctgttcgcga
    14641 tcctgaattt cgacccgcag cgccacccgt cttttcggat cgagcgtcgt cgcccacaac
    14701 tgctgcggca tcatctcacc caagcctttg aagcgctgga tttcgacttt ggcgtttggc
    14761 ttctgcttcg cgagcagctc atctttctgt ttgtcgtcga gcacccagtg ggtttctttg
    14821 ccgatatcga cgcgatacag cggcggctgg gcgatgaaca catgcccgtt ttcgataagc
    14881 tttgggatgt agcggtaaat gaacgtcaaa agcagcgtcg agatatggtg cccgtcgctg
    14941 tcagcgtcca tcagcaaaaa gattcgcccg taacgcagcc ggccttcgtc gtatttgtcg
    15001 cccatcccgc agccgagcgc cgcgacgatg tcttgcagct cgcggtttga caacagcttc
    15061 gacagcgatg cttgctcggc gttgagcact ttgccgcgca gaggcaaaat cgcctggttt
    15121 ttgcgattcc gaccctgttt ggccgatcca cctgcggagt caccttcgac gataaacagc
    15181 tcggcttgac ccggatcggt ggattggcag tcggccagct tgcccggcag attcagccgc
    15241 tgtccgacga cagatttgcg cggaaccgcc tggctcgccg ctttcatcgc ttcccgcgca
    15301 cgcgccgcag cgacgatgcg tgaaacaatc gcttcagcgg tctgcttgtt gctgttgagc
    15361 caattttcga caaacgtgcg caccgctccg tcgacttcgg cggtgacttc gggattgttg
    15421 agccgctctt tggtctggcc ttggaactgc ggttcgtgga tgtagcagct caagatcgcc
    15481 agcagacctt cacggatgtc ctcggcggtg agctggagac cttttggcaa aagtccgtgc
    15541 gtgtcgacga aattgcgcac gcctttgttg atgccagcct tgagcccgtt ttcgtgcgta
    15601 ccgcccgatg gtgtcgggat gccgttgacg aagctcttga tgctttcgtc ggtgccttca
    15661 gtccacaaaa acgacagctc aaaccgcgtc gtcccttcgt cggcgtcgcg gaaaaaatag
    15721 accggcggac acagcaccgg cttgccacgc tccgtcgccc acttttgcag gtaatcggca
    15781 atgccgcctt tgtgggaaag ctcgacggtt tcgccgcttg cttcgtcggt aaaaacgatg
    15841 cgcagacctt tgtgcagata gcttttggct tccaggcgct cgcgcacaag ttccaggtca
    15901 aacgacgctt tttcgccgaa aatttccgga tcgggccgga aatagacttt gctgccggaa
    15961 cctttggcgg caccgacgct gcgcagtggc gtcaccggga tgccgcgcgc atagccctgc
    16021 tcgacctctt tgccatcgcg acggacgcgg acccacagct cgacggacag cgcgttgacg
    16081 acggaagcgc cgacgccgtg cagaccaccc gagtgggtgt agctgccctg ctgaaacttt
    16141 ccgcccgagt gaagcgtcgt caagatcact tcaatcgcac ttttgccgta tttgggaagg
    16201 attcccgtcg gaaaaccacg tccattgtcg tcgacggtga cggaccgccg atcgctgtgc
    16261 aggatgactt tgatcgtggt cgcgtggccg tttatgacct cgtcgatggc gttgtcgagg
    16321 atttcccaca acaagtggtg atagccacga ctgtcgacac cgccgatgta cataccaggg
    16381 cgcttgcgga caggctcaag tccctcgagg acgacgatat cttcttcggt gtagcgcgga
    16441 ggcgtttggt tctgtgggct catggtaaaa gcgacagaaa agcgcggcca acgtagcacg
    16501 gctttggctg ccgtcaaaaa tggctcaagc ccgcgttaca ctgctttcca catcatgaac
    16561 ccgcccagcc tggacgcact cgaacacgcc aagcgcaccc ttatcgacgt cggcatccac
    16621 tacggcccac gccttgtcgt cgcgatgctg atcctgattt tcggcggttt tgtcgcccat
    16681 cgcgccggga tcatcagtcg tcgctggttt agcaaactcg agctcgatca gccgctcggg
    16741 caactgctgg gccgggcgct gacggtgctg gtgtttttgc tgtttctgac gctggcactg
    16801 caaaaccttg gggtggagct gctgccgctg tttgccagcc tcggtgtcgc aggcgttggc
    16861 gtcggcttgg cggcgcaggg cgtgctcggc aacctcgtcg ctgggcttac gatcattttc
    16921 acccgaccgt tccggattgg agagtgggtg cagatgatcg gcgtcgaagg acaagttgag
    16981 tcgatcgatc ttttttcgac gacgctaagc catcccgacc gatcgctggt ggtgattccc
    17041 aatcgaaaaa tcgtcggtga agtgctgcac aactacggca agctgcggca gctcgacctg
    17101 ctgattggcg tcgcttattc gacggacatt cagaaagccc tgatggtggt gcgggacgtg
    17161 ctgacacaaa acgaccgcgt caccaaagac atcgcgccca tcgtcggtgt gacgctgctc
    17221 ggggaaaagt cggtcgggat tgcgataaag ccgtgggtgg cggtgacaga ctacgtgcct
    17281 gccgtcggag agctaaacca agcgctggtc gaggcgtttc gcacccaccg gatcgagatt
    17341 tcgtttccgc aaagggacgt gcggatcgtc ggaagtgtcg tcgcctaagc cgcgccagct
    17401 acttgttcat tcggaacagc gccggatcga gcttttcatc gagcgacgct tcttcgacat
    17461 caatcgacac tttgtaattt agcgccgtgt tgtcgatctc gacgtgggtt ggcaccgaac
    17521 gaccctgcac ttccttgaac tgctggcagc ggatcgtgcg ctcgttttcg acgacgcgca
    17581 gctcgcccac tttcagatca acgtactttt tgtgcgacgg atggagcggt tccaggtcaa
    17641 acatcgccga cagatccgca ccgatcgaca agagcacttt ttgctgcaag ctgctgtctg
    17701 cgccggccat gctggtcacg actttgacgt cgccgccgac tttgatgaga tcgaacagcg
    17761 tgatgccgcc cggcccgatt gccttgagac ggaaacgatc cggcctttcg acggcgatca
    17821 tgccacgcac agtcacttgc tcgcgaccgt cgcctttggc agcgtcgatg ccgacgcgat
    17881 ggaccgcccg catggtgccc cagcgaatgt cctgaactgg ctgcaccgac gggcccttga
    17941 tgatgattgg cttgttgctg ttgtcgcagc cgctctggct tagcatcgcg acgcaggcgg
    18001 tcgcacacag cacacgcacc acccgacgcc acgcaccgcc acgcttgcca accgcactct
    18061 gcgcgaggtc gcgcgaaacc gtctcgtaca tgctcacagg gcctccgtca ttccgtgggg
    18121 catcgcgccc gcctctgggt ctac
//
LOCUS       DAORGN010000093        18092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1254_length_18092_cov_6.138992, whole genome shotgun sequence.
ACCESSION   DAORGN010000093 DAORGN010000000
VERSION     DAORGN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 18092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18092
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_18092_cov_6.138992"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKL17_11650"
     CDS             complement(<1..1001)
                     /locus_tag="PKL17_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNK45433.1"
                     /translation="MSFRFAHGLLLAFGSLLLSCDPPIPAQGSLPDMTVFSTRRLDPS
                     LSWWGDNRKRLDDFMVQSGIGSVGYDATKKPVALFDWDNTVIKNDIGDLMLFWMLKAG
                     KIYQPANFNWRLTSISITTDALNLLRAACDGLASPGSPLPTNRSDTAAIACTDAILSV
                     YLDNKGQTGLSAFAGYDYRRMEPTYAWAVQLQAGYTPAEISLMAKDAIAEGLAAAVGA
                     TQKIGSRTVNAYVRVYDQIKDLIGAMQDNGFDVWVITATSEPVVRAFADQVKIPTDHV
                     IGVRMVLDGNGKLTYNLQGCGDVPDGINDGGATAKGNSLMTYIDGKRCWVNKVIYGDA
                     "
     gene            1071..2897
                     /locus_tag="PKL17_11655"
     CDS             1071..2897
                     /locus_tag="PKL17_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK45434.1"
                     /translation="MPYVLRVIQKRSQSFAKSLFLGDLREELIFPFPQLTDADQRKLA
                     QLLSDVRAFSRQHIDSRRIDSEAKISPDLVDGLRKLGLFGLLLPTAYGGLGLSLSAAC
                     RVIDLLGSFDSSVAMLVAVHLGFAAQAIARFGTPEQKRYYLPKLCSGQSIGAFALAEP
                     MAGSDAASSRTRATPCDGGYRLTGTKTWVQNGEHADVLIVFAQTTVHRDGADVDRITG
                     FLVEKGPGVIIGHSEPKLGIRGSGTPALYLEDVFVPKDRVLGSLGGGFKVAMETRQHG
                     RLLAASACIGATCEILRHQVQQATSRRQFGRVIGTLGMIKDQVARTAVDLYASESALY
                     LTTGLQDLTDRHPERDLDTSLEAAMCKVLAAETIHNAAVAAMRLAGATGLLDSHPYER
                     LLRDSQTYLVFPGTHEVLRAYIALSGMREPGEQVGRLSDVLKRPLRGYGMVVDSLIEK
                     VRTAAYGRAILSRHHPQLKREAVLIEDAAESFAKEVDRVVRRHGQLLPEMQYVQKRVA
                     DVAIDLYAMCATVSRTSQVLGERDARHARGRAGHSEAAGQIDSGERQLRLCAAFCGKA
                     AKRIEQTLAQFGQNDDELMKTIADDCYLDRPYPFDALSKEPD"
     gene            3134..3577
                     /gene="dut"
                     /locus_tag="PKL17_11660"
     CDS             3134..3577
                     /gene="dut"
                     /locus_tag="PKL17_11660"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNK45435.1"
                     /translation="MPLLVVKLKPDAAVPAYKTAEAAGMDLSACLGAGEVLTLAPLER
                     RLVPTGLMVAIPAGYEGQVRPRSGWALKEGVTVLNSPGTIDSDYRGEVGVILINLSAQ
                     PVQIRHGDRIAQLLITPVARVNPVEVSSLDETARGAGGFGHTGRS"
     gene            3580..4734
                     /locus_tag="PKL17_11665"
     CDS             3580..4734
                     /locus_tag="PKL17_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45436.1"
                     /translation="MAAPLFRRGDVIGQKYYVLTKSGEAALWVGFLACSVELQKAAEE
                     RGEVPEPDLLVKVVRPELLESPGSADKLIRHLQSYKDLAHPFLTQVVDVVALPEQGTV
                     AITEIAQIGQGGQGVFLDRMAAFRQKEGLPLSDVLQIIDQLAESLTFLHEQGRYHGDL
                     RLDSVLLKTDGIRLGDVGLGVGLPREPFLLQLGTVGQMEAVAPELHAARPADIRTDVF
                     GLAKVMRSLLELGDNWTIIRDANQSLVHAISRAMATDPAERHPTIAALIADLEKALQP
                     EGSSLVSAPGDEKVDQPSTGSSPGSADAPSNDSAGFRSSRSSIGPEFPPGSVLVSWRA
                     FAATVVLAAVVGALLAVAVMLLRRSQLADGKKAVTAPPITVRSIPGSSQK"
     gene            4740..8525
                     /locus_tag="PKL17_11670"
     CDS             4740..8525
                     /locus_tag="PKL17_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNK45437.1"
                     /translation="MPTQFLAGLLVLFACVGATRAHSPFDQELTERRQRFAAEAARPG
                     SAAAVVPLLGLSDLWDDVVDRTTLREASTDATKPRPTVHELVRAEALWHDQMLRRHAG
                     DDAGARDAQRQLGLLTSFAICGPFDNDGRKGHDASYPPETETIAPNPSLRYLGKSPAL
                     PVSWRIVPDTALSRDGSVPIDAWMRPETEGTAYAVTYVKSEQRLAVAVRVGSSGAVKV
                     LVNQGPAVIDSSVYRKLHIDQEAGLATLLPGWNRVLVKVSSLSGRWAFVLRLTTPDGK
                     PLVGLTHSANPPTEGWKIPVAEKAQGPKPQTLIAALRARVAKAQPGSPQAAQRADQLT
                     DLGLYVHHAQPSDPEQHEAEALLAEAVTLRPSQRGFRYLADATEDENKQRLALEAGLA
                     LPTGGNAVERARLLVEIGTSYEQSGRLQKAEQAFSEAERLAPTLYTAVLGLCRVAQSR
                     GVASVGYKRISPLLLSAPSLTTLRAHADMLSRLGRHKEAESAYQVVLRQQPDDDEALR
                     YLISRRRAAGQIEDALQILAQLAAQRPDAQWPLRERLDILEGSGKHEDALQLVMAGLP
                     QHVGDAEWFERRGRLQKRLSRTELAVGSFRRALELKPQNPALREYLQTIDPAARSAED
                     LQRQFQVDVDSLIKKPRPATKPGDSARVLFEQKVVRIHTNGLSEEYIQRVVEILDEEG
                     ARIYDDASVTFSPDTQSVQFKTAKVIKPSGEVVESVSQSESDASEPWYGMYYDMHSVT
                     VRFDGLRPGDVVVVEHVRSDVGRRNLFADYFGDVTYLQESIPVLETRLLLTLPEDDLR
                     RRPLYFNQPKLPADATLARTDETQGKDHIVRFVAHNLPKIHDESGRPGYSELSAYIHV
                     STYKTWEDVATWYRGLVAESLLPSSEVTRAAQAVVAKIPASDELGRIRALYDEVVRRT
                     RYVGLEFGIHGYKPYKVPQVWERKFGDCKDKAALLHVMLKEVGIESSLTLARTTKGGN
                     IDPEPASLAVFDHAIVFVPKYNLYLDGTAEFSGSMELPSSDQDIMVLVVSDPRPPYSG
                     KGHLARTQVLPSHQSITRRVQTVQLEESGKAKVAEVLTVSGQNASQFRDHFQNPSSQK
                     ERYEKAWNDSFPGSQALSVEVPDVLNLEKPVTLHGELTVPKWGRPPSGGAAGTLSLRP
                     LGREPDLLRTHARLSSRRYDLVLGFAWVSQDEVTVHLPHRFAVHRLPESRNIESPFGR
                     FQLQIEQHPGKNGIAVTAKSELRIDRHRVAASDYPAFRTFLSEVDSALAQELLVGRE"
     gene            8518..12420
                     /locus_tag="PKL17_11675"
     CDS             8518..12420
                     /locus_tag="PKL17_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNK45438.1"
                     /translation="MSNRTFFTLAFSLWLGCGPQVPVKDRYLSSPPLDAQVADVSSKL
                     ARSSDPSPELHRELGLLLLVSGDGKKAEAQLDLALSQGARDSLTYLGAALAAAANGKP
                     HKSHQILLGFLSAQVQKEPTDPWAPAVAELAAHRLLSHGVSGTGKGDDKQLGDELFAL
                     WQKRSRLPVESQQLLAALLGQQLRLLGDEARANRVDIERGCPPVLYTSGPHGHLPQLD
                     VLSPLPGDSPLTDPQRPRYRQRSGSGCSVNVQGLPGKPGVFFVHSFFQTHSDKPIPLT
                     VETAGTPWALYIDGQRQFVDTEPVGRRYLQLQLPVGAHSLSVKLGVVGSNHVQVALPG
                     VQFYDGDPGKAPPHTPGEVKVSPRALSPLPMAETRWQGALAALLKMQQAYLSGQPDGG
                     LTVAAENMGQTPRFAALRTLYAGLLLDDHSRPERQARDRARSQLKQALGLSPELLSAR
                     QSLARLLLQDEKAALAQEVIDATPDSVEKTWQTELLRHRILKTRGFVVEAEAALSAAQ
                     KLGPSACPVLEHVADFRREQQDDRGALKAAQELTACNPYSDRFADALYDSGRLEEAQR
                     EYERLLQLEPDSTAWLRDLSRVYLARRDLGAAVSTLLKLRELLPRSTQLYTDLANLYV
                     ERGQPETALLQIKQGLAELPESADLAKALQALGHRDSIEPYRIDGRQVVREFEARHGV
                     YSGEPAVLLLDRTVVRVFPGGARIQLTHNIIRVLTKEGIERFGEVRIPDGAEVLQLRT
                     IKADGTTREPESIPEKDTVSAPALEVGDYVEYEYIDRDDPSPIAPGGFLADRFYFASA
                     DAPLDRSEFLLISPKDLPLQIDLRGPVGSDGKRQLPVATQKQDGDLSLHLWQRTGIAR
                     MQPEQPLDQGQVDDWSPSVRVGSGVTLAGYVNQIRERRYRALKLTPDIRELAQKVAGN
                     PDAAPESPQSQIRRVRALDEWVRKNINAGGSFDEPASSILARREGRRDVLLLALLKAA
                     GIAAETWLARPENNPKLEGPLPDALAYSEMLLAVAKDEKGTPLLWLDPLYRHTPTGQV
                     RPLLRGAQAIRLPDSLSEPKNQTVDLVRVDVPHAGGIPARLAQAQPDAKWPTLTDTRK
                     VELVAELEPSGSAKVVVTETLTGIQSSEWRDQVEHMAEDRLRQLLEQRALGYYFPGAS
                     LQALKYGPMDDDDAPLTLSYTFTAPHLARQRLGKDGKRMLVLPVPYPLLLSRRYVTTP
                     QRKLPLLVGYVTPGTLTATIKLPPGARIGQLADPVALSGFGSYKREVKAADGQVQLRV
                     ETNLPRQRIVPDRYPAFVDFAAHVDLADEAYALIELGP"
     gene            12497..13276
                     /locus_tag="PKL17_11680"
     CDS             12497..13276
                     /locus_tag="PKL17_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45439.1"
                     /translation="MLSRRRLPASIWAWAQKLSLLGTLFGFSGCDAELYHDLSERHAN
                     EAVMVLRQAGLHADKLATGRSARGPTFSLTVPPREQDQALAVLTDQGLPPVEPRPGMS
                     GKLAMLPGEVRAEQLAAREAALVDTLESLPSVSHARVHLTVAEPDPLLPTAQLRPTAA
                     VVLRTRGALLVKTSEVAELVAKSVTGLDAADVTVLVTQVATQAVLTAPARSGNLLVVG
                     LGGLCGLLTAAVVVLGILLRRRPNDPPPRSSESTLHALSSS"
     gene            13303..13803
                     /locus_tag="PKL17_11685"
     CDS             13303..13803
                     /locus_tag="PKL17_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNK45440.1"
                     /translation="MTQADQAGSTGPKKIDKILVPTDLSAFSEQVLDYALELAKSLGA
                     SIRLFHTAVLPDYEVPYLVSPGMRGAATALVEKAAEITAHIRTELEAICQRKYVYGVN
                     IEYSMTEGRPAEAICALASEMHADIIIMGSHSRPGLRHVFLGSVAEKVLRSAPCPVLI
                     VHPKSE"
     gene            13894..15009
                     /locus_tag="PKL17_11690"
     CDS             13894..15009
                     /locus_tag="PKL17_11690"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNK45441.1"
                     /translation="MQITANTTGMLADLAVVADPDAVDHCVVAIKGTFVTDQNGRLTL
                     ADHQQPLTTVDEHYGEPGSSCVRYECDFALYKPYTDVIVVGKAVAPNGIPVTGLSVRL
                     LVQGRAKDILVYGQRHWVSTLAGVRVSEPEPFTEMPITFDRAFGGQDDTQGKDRVAVE
                     LRNPVGVGFQPHRHRADLTGWPVPNIESPGRAIDSPREPYEPAGYGCIGRSWQPRVAL
                     AGTYGESWRNERAPYLPADFDPRYFQCAPQDQQFPHFQGGEKIRCVHMAAPEVVEYTI
                     PTLNMPVRFRFRDVDVERQAVLDTVIVEPHRLRAMLVWRASVPLRKKLTALTEVQVGE
                     PPQTQTVRPIGARHGKPVFSRLALAVRYLRQQRGGGA"
     gene            15006..16046
                     /locus_tag="PKL17_11695"
     CDS             15006..16046
                     /locus_tag="PKL17_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45442.1"
                     /translation="MTSQVVSIEAAGMACPVGLTWQSACAALRAGVNRKTSSCYRDNQ
                     GREIIASFLHEPLPTDAAWQDRWLMLLTLAIRDALGTRTLAAMPIFVALPVSGHGAAF
                     DSAWVAQSLSNRLGLPLAASQVHVFAEGSAGGYAALQQGCLQVRRGTPCVVAAADSLL
                     SAQRLLPLSEQDRLLVEGNSDGFVPGEAAAAVLLGDDPRRALAHIRGLGFAREPSRID
                     NEIPLRADGMLAASQAALAEAALLPHDLDFRISDATGESFFFKEQSLLVTRLLRERKP
                     EFPLWLPAESLGDTGAAAGLCGLIWATAAWQRGYAPGPCALACAGNEQGARAAVVLQN
                     PRPGQTRHSVWR"
     gene            16069..17145
                     /locus_tag="PKL17_11700"
     CDS             16069..17145
                     /locus_tag="PKL17_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNK45443.1"
                     /translation="MGKKVFANGMEIAHKAGAGKVIAAFPDVCLSPPPPPAGPVPIPY
                     PNTSSATDLRDGSTTVQIGGKPLALKGQSFYQSSPLGNEAATRNFGAAVLTHTISGKT
                     YFQASSMDVLVEGKPVCRHLDITTCNHASYPGSTPPMPNAEGMSQGGVEGEDESKCPC
                     CKGQLHDNQKDPDTGKPYKRCKEEDWYGGAVKVHDKKVADMHKFLADKPGWASLPGNQ
                     LKIDETLRKQKDAHEAMAKLKEARTKSPPCENLHNPPNEGCGVHFKKTNPRPNTSDSG
                     ERARLGFTDGVRNECIRRYKAKGRPCTSGSEVCHKTPLAAGGCPNGPDNLVPKDVLSP
                     ECAAVDDAQTRLQGLSAQFLLGAK"
     gene            17145..18041
                     /locus_tag="PKL17_11705"
     CDS             17145..18041
                     /locus_tag="PKL17_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45444.1"
                     /translation="MRRYYRRLLLPAGTGFLFGDAVPSDLASIRYLHGSDPEDVICSV
                     LAYAQQGQFAPCSRLLDLMAQHNDADIWGSCSTLLAFAAPRSILQQLIPLMERLRMEH
                     GTDAPLQWAGETLARSREAWVVPAVLRLFEKIQSHERFMALPVYLSMLLEPEPAGIDA
                     GPSQRPVVEEPDWYQPTPTWAISEYVRKVEGCRSTLVSSLSTPDRACLFDGAILSLRQ
                     VAMRTIRRIQLHEESALRIAEARLLLEAYTGEDFSGFYDEGQHRLLPLRASALLEAFL
                     QTDTPERFEPGVRHFFGHRIPD"
BASE COUNT         3498 a         5815 c         5511 g         3268 t
ORIGIN      
        1 gcgtcgccgt agatgacctt gttgacccag cagcgcttgc cgtcgatgta ggtcatcaag
       61 ctgttgcctt tggccgtcgc tccgccgtcg tttatgccgt ctggaacatc gccgcagcct
      121 tgcaggttgt aggtcagctt gccgtttccg tcgagcacca tccgcacccc gatcacatga
      181 tcggtcggga ttttcacttg atcggcaaag gcccgcacca ccggctcgct cgtcgcggtg
      241 atcacccaca cgtcgaagcc attgtcctgc atcgccccga tcaggtcttt tatctggtcg
      301 tagacccgca cgtaggcatt caccgtccgc gaaccgattt tctgcgtcgc gccgaccgcc
      361 gccgccagac cttctgcaat ggcgtctttg gccatcagcg aaatttccgc cggcgtgtat
      421 cccgcctgga gctgcaccgc ccaagcgtag gtcggttcca tccgacggta gtcgtagccg
      481 gcaaacgccg acagccccgt ctggcccttg ttgtcaagat agacgctcaa gatcgcatcg
      541 gtgcaagcaa tcgccgccgt atcgcttcgg ttcgtcggca aaggcgagcc cggactggcc
      601 agtccatcac acgccgcccg cagcaagttc agcgcatcgg tggtgatgct gatgctggtc
      661 aaacgccagt tgaagtttgc gggctggtaa attttccccg ccttcagcat ccagaacagc
      721 atcagatcgc cgatgtcgtt cttgatcacg gtgttgtccc agtcgaacag cgccaccggt
      781 tttttcgtcg cgtcgtaacc gacactgcca atgccagact gaaccatgaa atcgtcgagc
      841 cgcttgcgat tgtcccccca ccagctgagc gatgggtcga gccgcctcgt cgaaaacacc
      901 gtcatgtcgg gcagggaacc ctgggcaggg atcggcgggt cgcacgacaa aagcagcgac
      961 ccaaatgcca gcaaaagacc atgggcgaaa cgaaacgaca tcccggacct ccgtcgaaag
     1021 acacaaacgc gccgaagcgt aacagccgag atttgataag ccaagaaacc ttgccgtatg
     1081 ttttgcgggt gattcagaaa cgatcccaga gcttcgccaa gagccttttc ctcggtgatc
     1141 tgcgcgagga actgatcttc ccttttccgc agctcaccga tgccgatcag cgcaagctgg
     1201 cgcagctgct cagcgacgtg cgcgcgtttt cccgccagca catcgattcg cgacggattg
     1261 atagcgaagc gaagatttcg cccgatctgg tcgacggtct gcgcaagctg ggcctgtttg
     1321 gtctgctcct gccgactgct tacggcgggc tcgggctgtc gctgtcggcg gcgtgtcgcg
     1381 tgatcgatct gctcggttca tttgacagct cggtggccat gctcgtcgcg gttcacctgg
     1441 gttttgcggc gcaagcgatc gcacgctttg gcaccccgga acagaagcga tactacctgc
     1501 ccaagctgtg cagcggtcag agcatcggcg cgtttgcgct cgctgaaccg atggccggca
     1561 gcgacgccgc cagcagccgc acccgcgcca cgccatgcga cggcggctat cgactgaccg
     1621 gcaccaagac ctgggttcaa aacggtgaac atgccgacgt gctgatcgtg ttcgcccaga
     1681 ccaccgtgca tcgcgacgga gccgacgtcg atcgcatcac cggctttctc gtcgaaaaag
     1741 gccccggcgt catcatcggt cacagcgaac ccaagctcgg cattcgcggc agtggcacac
     1801 cggcgctgta tctcgaagat gtgtttgtgc ccaaagaccg cgtgctcggc agcctcggtg
     1861 gtggattcaa ggttgcgatg gaaacccggc agcacggacg actgctcgcc gcatcggcct
     1921 gtatcggtgc aacctgtgaa atcctccgcc accaagtcca gcaggcgacg agccgtcggc
     1981 agtttggtcg cgtcatcgga acgctcggga tgataaagga ccaagttgcg cgcaccgcgg
     2041 tcgacctgta cgcaagtgaa tcggcgctgt acctgacgac cgggctgcaa gacctgaccg
     2101 accggcaccc cgagcgcgac ctggatactt cgctcgaagc cgcgatgtgc aaagtgctgg
     2161 ccgccgagac catccacaat gcggcggtgg cagcgatgcg cctggccgga gccacggggc
     2221 ttctcgacag ccatccctat gaacggctgc tgcgcgacag ccagacgtat ctggtcttcc
     2281 ccggcacgca cgaggtgctg cgcgcgtaca ttgcgctttc gggcatgcgt gagccgggcg
     2341 aacaagtcgg gcgactttcg gatgtgctca agcgtcccct gcgcggctac ggcatggtcg
     2401 tcgattcgct gatcgaaaaa gtgcggacgg cggcctatgg acgggcgatt ctgtcccgcc
     2461 accatccgca gctgaagcgc gaagccgtgc tcatcgaaga tgccgccgag tcattcgcca
     2521 aagaagtaga tcgcgtcgtt cgtcgccacg gccagctgct gccggaaatg cagtatgtgc
     2581 aaaagcgcgt cgccgatgtc gcgatcgatc tgtacgcgat gtgtgcaacc gtttcacgga
     2641 catcgcaggt gctgggcgaa cgggatgcgc gtcatgcccg tgggcgcgcc ggtcacagtg
     2701 aagccgccgg acaaatcgac agtggcgaac ggcagctccg tctgtgtgcc gcgttctgtg
     2761 gcaaagccgc caagcgcatc gagcaaacgc tggcccagtt tggccaaaat gacgatgagc
     2821 taatgaagac catcgccgac gactgttacc tcgacagacc ctacccgttc gacgcgctca
     2881 gcaaagagcc agactgatca cgcgcacctg cccacaattg ggcaaaactt cgcacagtct
     2941 gcggcacgag cgctgcgact tgctggggac cgcacggcct ctttggcaca aagaaccgca
     3001 cttgcgccgc gacttccgct agaaatgccc ggtttgcggt taacataaaa aggagtttcc
     3061 cttggccaca cccacgcaac accctgccca gcttgaggat tcttgtacgg agcatgcgcg
     3121 cccgtcgacg caactgccac ttttggtggt gaagctgaaa ccggacgccg cggtgcccgc
     3181 ctacaaaacc gctgaagcgg caggaatgga tctgtcggcg tgtctgggcg ctggggaagt
     3241 gctgacgctg gcgccgctgg aacggcggct ggttcccacc gggcttatgg tcgcgattcc
     3301 ggcaggttac gaaggccagg tccgtccccg ctcgggctgg gcgctcaaag aaggcgtgac
     3361 ggtgctaaac agccccggca ccatcgacag cgattatcgc ggcgaagtcg gggtgatttt
     3421 gatcaatctg tcggcgcagc cggtacaaat tcgccacggc gaccgcatcg cccagctgct
     3481 catcacgccg gtggcccgcg taaatcccgt cgaagtcagc agcctcgatg agaccgcgcg
     3541 cggggccggc ggttttggcc acacaggccg gagctgagca tggcagcgcc tcttttccgt
     3601 cgcggcgacg tgatcggcca aaagtattac gtgctcacca agagcggtga ggcggccctg
     3661 tgggtcggct ttttggcatg cagcgtcgaa cttcaaaaag cggcggaaga gcgcggcgaa
     3721 gtgccagagc ccgatctgct cgtcaaagtg gtgcggcccg agcttctgga atcgcccggc
     3781 tcggcggaca agctgattcg gcatcttcag tcgtacaaag acctggcgca tccgtttttg
     3841 acccaagtcg tcgatgtggt ggcgctgccc gaacaaggca ccgtcgcgat caccgaaatt
     3901 gcgcagattg ggcaaggtgg ccaaggcgtc ttcctcgatc gaatggcggc gtttcggcaa
     3961 aaagaaggtc tgccactgtc cgatgtgctt cagatcatcg atcagctcgc cgaatcactg
     4021 acgtttctgc acgaacaagg gcgctaccac ggcgacttgc ggctcgattc ggtgctgctc
     4081 aagaccgatg gcatccggct tggcgacgtc gggcttggcg ttgggctgcc gcgtgaaccg
     4141 tttttgctcc agcttggcac cgtcggacaa atggaagcag tggccccgga attacacgcc
     4201 gcccgtcccg ccgacattcg caccgacgtg tttggactgg ccaaagtgat gcgttcgctg
     4261 ctggagctcg gcgacaactg gacgatcatt cgcgacgcca accagtcgct cgtccacgcg
     4321 atcagccggg cgatggccac agatcctgcc gaaagacatc cgacgattgc ggcactgatc
     4381 gctgacttgg aaaaagcgct ccagccggaa ggatcatcct tggtcagcgc accgggggat
     4441 gaaaaagtgg accagccatc gacgggaagc agccccggat ctgccgatgc gccgtcgaat
     4501 gactcagccg gctttcgttc atcgcgatcg tcgattgggc ccgagtttcc gcccggatcg
     4561 gtcctggtaa gctggcgcgc atttgcagcg accgtggttc tcgccgccgt cgtcggagcg
     4621 ctgctcgcgg tggcggtgat gctgctgcgc agatcgcaac ttgccgacgg aaagaaggcc
     4681 gtgacagccc cgccgattac cgtgcgttcg atccccggtt cttcgcaaaa gtagagaaca
     4741 tgccgaccca atttcttgcc ggcctattgg ttcttttcgc gtgcgtcggt gcgacgcggg
     4801 cccacagccc atttgatcaa gagcttaccg aacgtcgaca gcgctttgcc gccgaagcag
     4861 cacgtccagg gtcagccgca gccgtcgtcc ctctgcttgg cctgtccgat ctttgggatg
     4921 acgtcgtcga tcgcacgacc ctgcgcgaag catcgaccga tgcgaccaag ccacgaccga
     4981 cggtacatga actggttcgc gccgaagcac tgtggcacga tcagatgctc cgacggcatg
     5041 ccggcgacga tgccggggcg cgcgatgccc agcgtcagct tggacttttg acttcgtttg
     5101 cgatctgcgg cccgtttgac aacgacggtc gcaaaggaca cgacgcaagc tatccgcccg
     5161 aaaccgaaac gatcgcacca aatccgtcgc tgcgctatct gggtaaaagc cccgcgctgc
     5221 cggtttcatg gcggattgtg cccgacaccg cactttcacg cgatggcagc gttcccatcg
     5281 atgcgtggat gcgtcccgaa accgaaggca ccgcctatgc cgtgacgtat gtcaaaagcg
     5341 agcagcgctt ggccgtggcc gtgcgcgtcg gatcgagcgg cgccgtcaaa gtgctggtca
     5401 accaaggtcc ggcggtgatt gattccagcg tctatcgcaa gctgcacatc gaccaagagg
     5461 cgggtctggc gacgctgctg cccggctgga accgtgtgct cgtcaaagtg tcgtcgctaa
     5521 gtggccgctg ggcattcgtg ctgcggctga cgacccccga tggcaagcca cttgtcggtc
     5581 tgacccacag cgccaacccg cccaccgaag gctggaaaat tcccgtcgca gaaaaagccc
     5641 aaggtccaaa gccgcagacg ctgattgccg ccctgcgcgc ccgcgtcgca aaagcgcagc
     5701 caggcagtcc gcaagctgcg cagcgggccg atcagctgac cgacttggga ctttatgttc
     5761 accacgccca gccaagcgat cccgaacagc atgaagccga agcgctgctg gccgaagcgg
     5821 tcaccctgcg tccatcgcag cgcggctttc gctatctggc cgatgcgacg gaagatgaaa
     5881 acaagcagcg gctggcgctc gaagcgggtc tggctttgcc gaccggcgga aatgcagtcg
     5941 agcgcgcgcg gctgctggtt gaaatcggaa ccagctacga acagtctggc cgcctgcaaa
     6001 aagccgagca agcgttcagc gaagccgagc ggctggctcc gacgctgtat accgcggtgc
     6061 tgggtctttg ccgcgtggcg caaagtcgcg gggtggccag cgtcggttac aaacgaattt
     6121 caccgctgct gctttcggcc ccgtcgctca cgacgctgcg tgcccacgcg gacatgctgt
     6181 cgcggctggg acgacacaaa gaagcggaat cggcgtatca ggtggtgctt aggcaacaac
     6241 ccgacgatga cgaagcgctg cgctacctga tttctcgtcg tcgagctgcc ggccaaatcg
     6301 aagacgcgct ccagatcctg gcgcagcttg cggcccagcg tcccgatgcg cagtggccgc
     6361 tgcgtgaacg gctcgacatc ttggaaggca gtgggaagca cgaagatgcg ctccagttgg
     6421 tgatggccgg actgccccag catgtcggcg atgccgagtg gttcgagcgt cgcggacggc
     6481 tgcaaaagcg gctttcgcgc accgagctcg ctgtcggatc gttccgtcgc gcgctggagc
     6541 ttaagccgca gaatccggcg ctgcgcgaat acctgcaaac catcgaccca gcggcgcgat
     6601 ccgccgaaga cttgcagcgc cagttccagg tcgatgtcga ttcgctgatc aaaaaaccgc
     6661 ggcccgccac aaagcccggc gacagcgccc gcgtcctgtt tgaacagaaa gtggtgcgaa
     6721 tccacaccaa cggcctgtcg gaagaataca tccagcgcgt cgtcgagatc ctcgatgaag
     6781 aaggtgcgcg catctacgac gacgccagcg tcacgttttc ccccgacacc caatcggtgc
     6841 agtttaagac cgccaaagtc atcaagccga gcggtgaagt ggtcgaaagc gtatcgcaga
     6901 gcgagagcga tgcgtcggag ccgtggtacg gcatgtacta tgacatgcac tcggtgacgg
     6961 tgcgttttga cggcctgcgg cctggcgatg tcgtcgtcgt cgagcacgtt cgatccgatg
     7021 ttggtcgccg caacctgttt gccgactatt ttggcgacgt aacgtacctt caggaaagca
     7081 tcccggtgct agaaacccgg cttttgctga cgctgcccga agacgacctg cgccgtcgcc
     7141 cgctgtattt caatcagccg aaactgcctg ccgacgcgac gcttgcacgg accgacgaaa
     7201 cccagggcaa agatcacatc gttcgctttg tcgcccacaa cctgcccaag atccacgatg
     7261 aatcgggccg tccgggctat tccgagctgt cggcgtacat ccacgtctcg acgtacaaga
     7321 cctgggaaga tgtcgcaact tggtatcgag gactggtcgc cgaatcgctt ctgccatcgt
     7381 ccgaggtcac tcgcgccgcc caagccgtcg tcgccaagat tccagccagc gatgaactgg
     7441 gccgaatccg cgcgctctac gacgaagtgg ttcgccgcac ccgctacgtc gggctggagt
     7501 ttggcatcca cggctacaaa ccctacaaag tgccgcaggt ctgggaacgc aagtttggcg
     7561 actgcaaaga caaagcggcg ctcctgcacg tcatgctaaa agaggtcgga attgagagct
     7621 cgctgacgct ggcgaggacg accaagggcg gcaacatcga tcccgagccg gcgtctttgg
     7681 cggtgttcga tcacgcgatt gtgtttgtcc ccaagtacaa cctgtacctc gacgggacgg
     7741 cggagttttc gggatcgatg gaactgccca gctccgacca agacatcatg gtgctggtgg
     7801 tcagcgaccc gcgcccgccg tattcgggca aaggccacct ggcgcggacc caagtgttgc
     7861 cgtctcatca gagcatcacc cggcgcgtgc agacggtgca gcttgaagaa tcgggcaaag
     7921 ccaaggtcgc cgaagtgctg acggtgtccg gacaaaacgc cagccaattc cgtgaccact
     7981 tccaaaatcc gtcgtcgcag aaagagcgct acgaaaaagc ctggaacgat agcttccccg
     8041 gcagtcaggc gctttccgtc gaagttcccg atgtgctgaa cctggaaaaa cccgtcacgc
     8101 tgcacggtga gctgacggtg cccaagtggg gtcgtccgcc cagcggtgga gcggcgggca
     8161 cgctgtcgct gcgaccgctc ggacgggaac cggatctttt gcgcacccat gcgcggttgt
     8221 cgtcgcgacg ctacgacttg gtgctgggtt ttgcgtgggt cagccaagac gaagtgacgg
     8281 tgcatctgcc gcatcgcttc gccgttcatc gcctgccgga atcgcgcaac atcgaatcgc
     8341 cctttggtcg cttccagctt cagatcgagc agcatccagg caaaaacggc atcgctgtga
     8401 ccgccaagtc cgagctgcgg atcgaccgcc atcgcgtcgc cgcgagcgat tatccggcgt
     8461 tccgcacctt cctttccgaa gtcgatagcg cgctcgctca agagcttctg gtgggccgtg
     8521 agtaaccgaa cttttttcac actggcgttt tcgctgtggc tcggctgcgg accgcaggtt
     8581 ccggtaaagg atcgctacct cagcagtccg ccgctcgatg cgcaggtcgc ggatgtatca
     8641 agcaagctgg cacgcagttc cgacccttcg ccagagcttc atcgcgagct tggactgctt
     8701 ttgctggtat cgggggacgg gaaaaaagcc gaagcgcagc tcgatctggc gctgtcgcag
     8761 ggggcgcggg acagcctgac ctatctgggc gcagcgctcg cggcggcagc aaacggcaag
     8821 ccgcacaagt cgcatcagat cctgctcggt tttctttcgg cgcaggtgca aaaggaaccg
     8881 accgacccgt gggcacccgc ggtggccgag ctggctgcgc accggctgct ttcgcacggc
     8941 gtctccggca ccggcaaagg cgatgacaaa cagcttggcg acgagctgtt tgcgctgtgg
     9001 caaaaacggt ccaggctgcc ggttgaaagt cagcagcttc tggcggcgct gcttgggcaa
     9061 cagctccggc tgctcggcga tgaagcccgc gccaaccgcg tcgacatcga gcgcggctgc
     9121 ccgccagtgc tttacacaag cggtccgcac ggtcacttgc cacagctcga tgtgctctcg
     9181 ccgctgcccg gagacagtcc gctcaccgat ccgcaaagac cccgctacag gcagcgatcc
     9241 ggctcgggtt gcagcgtgaa tgtgcaaggg ctgcccggca aacccggcgt gttctttgtc
     9301 cacagctttt tccagaccca cagcgacaag cccatcccgc tgacggtcga aactgccggc
     9361 actccgtggg cgctatacat cgacggacag cggcagtttg tcgataccga accggtgggg
     9421 cgacgctatc tgcagcttca gcttccggtc ggggcgcact cgctttcggt aaagcttggc
     9481 gtcgtcggca gcaaccacgt ccaggtggcg ctgcccggcg tgcagtttta cgacggcgat
     9541 cccggcaaag caccgccaca caccccaggt gaagtgaaag tcagcccacg cgcgctgtcg
     9601 cctttgccca tggctgaaac tcgctggcaa ggtgcgctgg cggcgctttt gaaaatgcag
     9661 caggcctatc tgtcgggtca gcccgatggt ggtctgacgg ttgcggcgga aaacatgggc
     9721 cagactccgc gctttgcggc gctgcggacg ctgtacgccg ggcttttgct cgatgatcac
     9781 agccggcccg agcgccaagc cagggatcgg gcgcgctcgc agcttaagca ggcgcttggc
     9841 ctaagccccg aacttctgtc ggcgcggcag tcgctggccc ggctgctttt gcaagacgaa
     9901 aaggcggcac tcgcgcaaga ggtcatcgac gccacacccg attcggtcga aaagacctgg
     9961 cagaccgagc ttctgcgcca tcgtatcctc aagacccgcg gctttgtcgt cgaagccgaa
    10021 gccgcgctga gcgctgccca aaagctgggt ccgtcggcgt gtccggtgct ggaacatgtg
    10081 gctgatttcc gccgcgaaca gcaagacgac cgcggcgcgc tcaaggccgc gcaggaactg
    10141 accgcctgca acccctattc ggatcgtttt gccgatgcgc tctacgacag tggacggctc
    10201 gaagaagcgc agcgcgaata cgaacgactg ctccagcttg aacccgacag cacagcctgg
    10261 ctgcgtgatc tgtcgcgggt ctatttggcc cgtcgcgatc tcggggcggc ggtaagcacg
    10321 ctgctcaagc tgcgagagct tttgccgcgc agcacccagc tttacaccga tctggccaac
    10381 ctctacgtcg agcgtggcca gcctgaaacc gcgctgctcc agatcaagca ggggcttgcc
    10441 gagctgcccg agtctgcgga cctggccaaa gcgctgcaag ccctgggtca tcgcgactcg
    10501 atcgaaccgt accgcatcga cggcaggcaa gtggtccgcg agttcgaagc ccgacacggc
    10561 gtctacagcg gagagccagc cgtccttttg ctcgaccgca cggtggtgcg tgtcttcccc
    10621 ggcggtgcgc gcatccagct tacccacaac atcatccggg tgctgaccaa agaaggcatc
    10681 gaacgctttg gcgaagtccg cattcccgac ggagccgaag tgctccaatt gcgcacgatc
    10741 aaagccgacg gcaccacccg cgagcccgag tcgattcccg aaaaagacac ggtgtccgcc
    10801 ccagcgcttg aagtcggtga ctacgtcgaa tacgagtaca tcgaccgcga tgatccgtcg
    10861 ccgattgcgc ccggtggttt cctcgccgac cgcttttact ttgcctcggc agatgcaccg
    10921 ctcgatcgca gcgagttttt gcttatctcg cccaaagacc tgccgttaca aatcgacctg
    10981 cgtggtccgg ttggttcgga cggcaaacgg cagcttcctg tggcgacgca gaagcaggac
    11041 ggcgacctgt ctttgcacct gtggcagcgg accggcattg cccgcatgca gcccgagcag
    11101 ccgcttgacc agggacaagt cgacgactgg agcccgtcgg tgcgcgtggg ttcaggggtg
    11161 actttggccg gctacgtcaa ccagatccgc gagcggcgct atcgggcgct caagctgacc
    11221 cctgacattc gcgagctggc ccagaaagtc gccggcaacc ccgacgccgc accggaaagt
    11281 ccgcagagcc aaatccgccg cgtgcgcgcc ctggatgaat gggtgcgcaa aaacatcaac
    11341 gccggcggca gctttgacga accggcaagc tcgattctgg cgcggcggga aggtcggcgc
    11401 gatgtgctgc tgttggccct gctcaaagcg gcgggcattg ccgcggaaac ctggctggcg
    11461 cggcccgaaa acaaccccaa gctcgaaggc ccgctgcccg atgcgctggc ctattcggaa
    11521 atgctgctcg cggtggccaa agatgaaaag ggaacgccgc ttctgtggct ggacccgctg
    11581 tatcgccaca cgccgaccgg acaagtcagg ccgcttttgc gcggggccca ggcgattcgg
    11641 ctgcccgact cgctttctga accgaaaaac cagacggtgg atctggttcg ggtcgatgtg
    11701 ccacacgcgg ggggcattcc ggcgcgactt gcgcaagccc agcccgacgc caagtggccc
    11761 acgttgaccg acacccgcaa ggtggaactt gtcgccgagc tggaaccgag cggcagcgcc
    11821 aaggtcgtcg tcaccgaaac cctgaccggc attcaatcga gcgagtggcg cgaccaagtc
    11881 gagcacatgg cggaagaccg gctgcggcag cttctggagc agcgcgccct aggctactac
    11941 ttccccggcg cgagcctgca agcgctcaag tacgggccga tggacgacga tgatgcgccg
    12001 ctcacgctca gctacacgtt taccgcgccg cacctggcca ggcagcggct tggcaaagat
    12061 ggcaagcgga tgttggtcct gccggtgccg tatccgcttt tgctaagccg ccgctacgtg
    12121 acgacgccac agcgaaagct gccgcttctg gtcggttacg tgacgccggg cacgctgact
    12181 gcgacgatca agctgccgcc gggagcgcgg attggccagc tcgccgatcc cgtggcgctt
    12241 tcgggctttg gcagctacaa gcgtgaggtc aaggccgccg atggtcaggt gcagctccgg
    12301 gtcgaaacca acctgcctcg ccagcgcatt gtgcccgacc gctatccggc ctttgtcgac
    12361 tttgccgcgc atgtcgatct ggccgacgaa gcctacgcgc tcatcgagct gggaccgtaa
    12421 gccgcgcctg tcccccacgc ccgccgcctg ccaaagtccc gtgacagctg ggcatttctc
    12481 ggattagcat aaagtcgtgc tctctcgtcg tcgattgcca gccagtatct gggcttgggc
    12541 gcaaaagctt tcgctgcttg gcaccctgtt tggtttcagc ggctgcgacg ccgagcttta
    12601 ccacgacctg tcggaacgcc acgcaaacga agcggtgatg gtgctgcgtc aagccggact
    12661 tcacgccgac aagctggcga cgggacgatc ggcgcgtgga cccacttttt cgctgacggt
    12721 gcccccgcgt gaacaagatc aagcgctggc ggtactaacc gaccaaggcc tgccacctgt
    12781 cgagccgcgc ccagggatgt cgggcaagct ggcgatgctt cctggcgaag tgcgggccga
    12841 gcagctcgct gcccgcgaag cggcgcttgt cgatacgctg gaaagtctgc cgtcggtcag
    12901 tcacgcgcgg gtgcatctga cggtggccga gcccgaccct ctgctgccga ccgcccagct
    12961 taggccgacc gcagcggtgg tgctgcgaac acgcggcgcc ctgttggtca aaaccagcga
    13021 agtcgccgag ctggtcgcca aaagtgtcac tgggctcgat gccgcagacg taaccgtgct
    13081 ggtgacgcaa gtggcgacgc aagcggtctt gaccgcgccg gctcgcagcg gaaatctgct
    13141 ggtggtggga ctcggtgggc tgtgcggtct actcaccgca gcggtggtgg tcctgggcat
    13201 tctgctgcgc cgtcgaccaa acgacccacc gccgcgcagc agcgagagca ccctccacgc
    13261 gctctcaagt tcatagcgaa tctctggtat acggagcaga ccatgacgca agcagaccaa
    13321 gcgggcagca caggcccaaa gaagatcgac aaaatcctcg tgccgaccga tctgtcggcg
    13381 ttttccgagc aggtgctcga ctacgcgctg gagctggcca agtcgcttgg cgccagcatt
    13441 cgcctgttcc acaccgcggt gctgcccgac tacgaagtcc cgtacttggt gagccccggc
    13501 atgcgtggcg cagcaacggc gctggtcgaa aaggccgccg aaatcaccgc ccacatccgc
    13561 accgagctgg aagcgatctg ccagcgcaag tacgtctacg gcgtcaacat cgagtacagc
    13621 atgaccgaag gccgccccgc cgaagccatc tgtgcgctcg ctagcgaaat gcacgccgac
    13681 atcatcatca tgggcagcca cagccgtccc ggcctccgcc acgtcttcct cggctccgtc
    13741 gccgaaaaag tcctccgcag cgccccctgc cccgtcctca tcgtccaccc caaaagcgaa
    13801 taaccccacg cgccccatgg cttcgcgtgc ggcctttggt atcgggcccg tcttagccta
    13861 acgtcttggc ccacacaaac acctgaccgc aacatgcaga taacagccaa caccaccggc
    13921 atgcttgccg atttggcggt ggtcgcagac cccgacgcag tggatcattg cgtggttgcg
    13981 atcaaaggca cgttcgtcac cgatcaaaac ggtcggctca cgctggcaga tcaccagcag
    14041 ccgctgacga cggtcgatga gcactacggt gagccgggca gctcctgcgt tcgctacgaa
    14101 tgtgacttcg cgctgtacaa accgtacacg gacgtgatcg tcgtcggaaa agcggttgcg
    14161 cccaacggga ttccggtcac cggattatcg gtacggctcc tggtgcaagg gcgagcgaaa
    14221 gacatccttg tgtacgggca gcggcactgg gtttccacgt tggccggcgt ccgggtgagc
    14281 gagcctgagc cattcaccga gatgcccatc accttcgacc gcgcttttgg tggccaagac
    14341 gatacccagg gaaaagaccg cgtggccgtc gagctgcgca accctgtcgg cgtcggattc
    14401 cagccgcacc gacaccgcgc cgacctgacg ggatggccgg tccccaacat cgaatcgccc
    14461 gggcgggcca tcgattcccc gcgcgaacca tacgaacctg ccgggtatgg ctgcatcggt
    14521 cgatcctggc agccgcgtgt ggcgctcgcc ggaacctacg gggaaagctg gcgcaacgaa
    14581 cgggcgccgt acctgcccgc agacttcgat ccgcgctatt tccagtgcgc gccgcaggac
    14641 cagcaatttc ctcacttcca gggcggcgaa aaaatccgct gcgtccacat ggccgctccc
    14701 gaagtggtcg aatacaccat tccgaccctg aacatgcctg ttcgctttcg ctttcgcgac
    14761 gtggacgtgg aacgacaagc cgtgctcgac acagtgatcg tcgagccgca caggctgcgg
    14821 gccatgctgg tctggcgagc gagcgtcccg ttacgcaaaa agctcaccgc gctgaccgaa
    14881 gtgcaggtgg gagagccgcc gcagacccaa acggtccggc ccatcggggc acgccacggc
    14941 aagcctgtct tttcgcggct tgctttggcc gttcgttatc tccgtcagca gcgtggaggc
    15001 ggcgcgtgac ttcccaagtc gtttccatcg aagccgccgg catggcttgc cctgtgggtt
    15061 tgacttggca aagcgcctgc gccgccctgc gcgccggtgt aaaccgcaag accagctctt
    15121 gctatcgaga caatcaggga cgcgagatca ttgcgtcatt cttgcacgag ccgctcccca
    15181 ccgatgcggc ttggcaggat cgctggctga tgctcctgac actggcgatc cgcgatgcac
    15241 tgggaacgcg gacgctcgca gccatgccga tctttgtggc gctgccagtg tcgggacatg
    15301 gtgccgcttt cgattccgca tgggtggcgc agtccctgtc gaacaggctg gggcttccgc
    15361 ttgctgcgag ccaggttcac gtctttgccg aaggttccgc cggcggatat gccgcactcc
    15421 aacagggctg tctgcaggta cgccgtggta cgccatgtgt ggttgcggca gccgattcgc
    15481 tgctttcggc gcagcggctg ctgccactgt ccgaacagga ccgcctgctg gtcgaaggca
    15541 actccgatgg gtttgtaccg ggcgaagcag cggcggcggt gctgcttggg gacgacccgc
    15601 gccgggcttt ggcacacata cgtgggctcg gtttcgcacg cgaaccatcg cggatcgaca
    15661 acgaaatccc gctgcgcgcc gatggaatgc ttgctgcctc gcaagccgcg ctggcggaag
    15721 ccgcgctttt gccccacgac ctagactttc gcatctcgga tgcgacgggc gagagctttt
    15781 tcttcaaaga gcagtcactg ctggtcaccc gccttttgcg cgagcgcaag ccagagtttc
    15841 ccctgtggct tcccgctgag tcgctcggtg acacgggtgc cgctgcgggg ctctgcggcc
    15901 tgatctgggc caccgcggcc tggcagcgtg ggtatgcgcc tggtccgtgc gcgctggcct
    15961 gcgccggcaa cgaacaaggg gcacgcgcag ccgtggtgct gcaaaacccc aggccggggc
    16021 agacaaggca ctcggtttgg cgataggttc atcgagcgga gcacagaaat gggcaagaag
    16081 gtattcgcca acggcatgga gatcgcacac aaagcaggcg caggaaaagt catcgccgcg
    16141 ttcccagacg tctgtttgag cccgccgccg ccgcctgctg ggccggttcc cattccgtat
    16201 cccaacacct cgtccgccac agacctgcgt gacggcagca cgactgtcca gattggcggc
    16261 aaacctctcg ccctcaaagg ccagagcttt tatcaaagct cccctttggg caacgaagcg
    16321 gccactcgca acttcggcgc tgctgtcctg acccacacca tttctggaaa gacttacttc
    16381 caggcttcct ctatggacgt tctcgtcgaa ggcaaaccag tctgccgcca cctcgacatc
    16441 accacctgca accacgcaag ctaccccggc agcaccccac ccatgcccaa tgcggagggg
    16501 atgagtcagg gaggcgtcga aggcgaagac gagtcgaagt gtccctgctg caagggacag
    16561 ctgcatgaca accaaaaaga tccagacacc ggtaagccat acaaaagatg caaagaggag
    16621 gattggtatg gtggggccgt gaaggttcac gacaaaaaag tggccgatat gcacaagttc
    16681 ttggcggaca aaccaggctg ggcaagcctc ccgggcaacc agttgaagat tgatgagacc
    16741 ctgcgaaaac aaaaggatgc ccacgaagca atggccaagc tcaaggaggc gcgcaccaag
    16801 tccccccctt gcgagaacct ccacaatccg ccaaatgagg gctgtggcgt tcacttcaag
    16861 aagaccaatc ccaggcccaa cacatcggac tcaggcgaac gagcccgtct aggcttcacc
    16921 gatggcgtcc gcaacgaatg catccgacga tacaaggcga aagggcgtcc ttgcacgtca
    16981 gggtctgagg tatgccacaa gacaccgctc gctgccggcg ggtgtccaaa cggccccgac
    17041 aacctggtgc ccaaagatgt cttgagcccg gagtgcgccg cagtggacga tgcccagacc
    17101 cgtctacaag gtttgtcggc ccagttcctg ctgggagcga agtgatgcgc cgctactatc
    17161 gcaggctgct ccttcctgca ggaaccggct ttctctttgg agacgctgtc ccttctgatc
    17221 tcgcaagcat ccgttaccta catggatcag accccgagga cgtgatttgc tccgtgctgg
    17281 cttacgctca acaaggccag tttgcgccct gctcacgcct gctcgacttg atggcccagc
    17341 acaacgatgc ggatatctgg ggttcgtgtt cgacccttct ggcctttgcc gcgccacgat
    17401 ccattcttca gcagttgatc ccactcatgg agcggctgcg gatggagcac ggcacggatg
    17461 caccgctgca atgggcagga gagactctgg cgaggtcccg tgaagcatgg gtggtacctg
    17521 ccgtgcttcg ccttttcgaa aagatccagt ctcatgagcg tttcatggct ctccccgttt
    17581 acctctccat gctgcttgag cccgagccag ccggtatcga tgcgggtccg tctcagcggc
    17641 ctgtggtcga agaacccgac tggtatcagc cgacaccaac ttgggcgatc tccgaatacg
    17701 tgcgcaaggt agaagggtgt cgttctacgc ttgtttcttc gctttccact ccagaccgag
    17761 cttgtctatt tgacggagcg atcctctcct tgcgccaagt cgcgatgcga acaatccgac
    17821 gaattcagct ccatgaagag agcgctctgc gcatcgcaga agcacggctg ctgctcgaag
    17881 cctataccgg cgaggatttc tcaggcttct acgatgaagg ccagcaccgc ttgttgccac
    17941 tgcgcgccag cgcactgcta gaagccttcc tccaaacgga tacgccagag cgatttgagc
    18001 ccggcgtccg tcacttcttc ggccaccgca taccggacta gtacgatttc ccgggcgtca
    18061 tcgacggacg cacagggccg atgctgcgcc gg
//
LOCUS       DAORGN010000094        17905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1273_length_17905_cov_6.519048, whole genome shotgun sequence.
ACCESSION   DAORGN010000094 DAORGN010000000
VERSION     DAORGN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 17905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17905
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_17905_cov_6.519048"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKL17_11710"
     CDS             complement(<1..586)
                     /locus_tag="PKL17_11710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45445.1"
                     /translation="MSTASPDNADSATCSRCGATYKTGTVHICPLADPSTLTEDKLIG
                     TTIDNRYTVEGRLGEGGMGVVYRARHVTLRKPVAIKVLRKAQEESAWKRFIQEAQTAS
                     MINHTNVVDLTDFGMIEGHPYLVMEFLEGETLTEAITSGPIPPLRVCRIGAQIARGLQ
                     AVHDKGIVHRDLKPDNIFLLEREGRQDVVKIVDFG"
     gene            940..2397
                     /locus_tag="PKL17_11715"
     CDS             940..2397
                     /locus_tag="PKL17_11715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45446.1"
                     /translation="MIGTRVGPYRIERKLGQGGMGAVYEAVHETIERRVAIKVLNPQL
                     AQNSDVSVRFINEARAVNLVNHPGLVQVSDFGQMPDGTAYIVMELLEGETLSKRLARL
                     GGKLPLPEMLKLVRQISSALQAAHMKGIVHRDLKPDNVMLVTESDPETGTRERVKLLD
                     FGIAKMSASSQGENKSNTQTDAVLGSPEYMSPEQCKGGVNIDQKADLYSLGVMMYRML
                     SGRLPFNAEGVGALMAMHIYEKPPDLRPLAPDTPPDLIALVDKMLSKVPAERPTAAQV
                     QYEIEQLATRVGMPMVTSSMQAIRSQIMTAESMPESVRGISTLGGASGQAAVPAPPPK
                     SKAPLFVGLGVGLLVLVGAVVGALSLRKPDKPQAQVVMQQKQPDPPKETPPPPPEPKK
                     RPKWTVDSEPQGADIIRTDDGQLLGQTPWTGEQPDGSGPVEVTIHLTGFSDRKITLKP
                     GPDTSYKQTLKPKRGRKGKAGKNQDQKNDGPEIAD"
     gene            2372..3082
                     /locus_tag="PKL17_11720"
     CDS             2372..3082
                     /locus_tag="PKL17_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45447.1"
                     /translation="MMDQKSRTNALSSWRSGLCALALLAVSSPAVAAEQSSGDDGVAA
                     TDPCLTDNLCKGHYDEARKAYKDKHYDEAFKEYKSAYERRQAPWLLINIGRCSQRLGR
                     NKEALDYYQRYLKADPNPKPETVTRVNGYIDQVKAGIIDEPEAKPEPKPEAKPEPKPE
                     PQPQLTTAQQNTQATAEPEKTPVYKKWWFWTIIGVVVAGGVAGGVAGGVIASKNATPQ
                     VVSNPPVPSGVTVYMPSF"
     gene            3109..5577
                     /locus_tag="PKL17_11725"
     CDS             3109..5577
                     /locus_tag="PKL17_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45448.1"
                     /translation="MKNRFVRNGSRLCLLAGALLASSCSPSTVPDSQILIVVNVKGIQ
                     SGTQALQVKSSLDGRADPNGLTITNNTSKFAIQLANDPKNYGQLKLDGYALDNNQCYL
                     ANGQVTEQITAQKTYYELDLTLLPQASPKCSLTVNILSTGTETVTSSPAGINCGGGQT
                     TCSYDFPFGTAVTLSGPVSQASYPVWTGGCTPAATYYSATCVANVQKGNASATVDFVP
                     RVCSPDGWCQYHPLGTTQTLIDVWGADAKNLWIAGTSGTVLSSSGGAWLPSANPWGTN
                     QVSFVRGTNASNVYASGSSTTTSLMRWDGTKWSAQPALPNQINTNLLGMHVIGSSDVM
                     LSVADYTSAPTKYLIYRFNGTTYSSIGPTLGASEYLTRLWGFGGNDVWAGSSLGIYHF
                     DGTKWTKDTSTAIQNKIIQFIWGSATNDVWAATTAELFHWDGTTWTAAAPDNGVTPTN
                     ILGIGGGTGPDVWVTTSAANGRFYRYAGGSCSPKCWSQVDVTGVTTLIRSVWGATNND
                     LWAVGNTGLILHYDGQTWSRSQYQKVPLTTGALYAAYGVANNQTSAMQAYGPTDTALN
                     VDNTNIVPANGFSTATQSIFAAYGLNANEIMIVGSGGTGGGTVMRWDGTSWKTYPSGA
                     PNFAYLWGVYINNSPRYYYIVGEQGYIARADATLTGFTPMTKTVATSASFYAVGGSSY
                     QNLYAVGSGGTVYRSTDGMNFSPVTAPNNDTGQTYYSAFYHPGSGRVYIAGTNGNLWY
                     YTGAPWAQVNTASAATLYGMWGMSGSQALWLAGSNGTILKFDGTNVAALKTGTTSTLR
                     SIWGNNLTDIWAVGLSGTILRWKM"
     gene            complement(5624..6034)
                     /locus_tag="PKL17_11730"
     CDS             complement(5624..6034)
                     /locus_tag="PKL17_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNK45449.1"
                     /translation="MSKPIPKVQKYMTTSPHTVRPDMSLAEAAKIMREHNIRHLPVLS
                     GGSLVGLVSIRDIHLIETLKDVDPNNVRVEDAMSQEVYVISPESQLDEVVEEMAAKKY
                     GSAIVEQNGKVVGIFTVIDALLALSELLNTRLSK"
     gene            complement(6241..7230)
                     /locus_tag="PKL17_11735"
     CDS             complement(6241..7230)
                     /locus_tag="PKL17_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNK45450.1"
                     /translation="MARQWAKTRRPRALLLPHDNPDPDSLAAAAGLSLLLGRQGIECT
                     IGMAGIIGRAENRAMVRVLSFDLRPLENLDLDSFGIVALLDTQPGTGNNSLPAGRLPD
                     IVIDHHPPRPISAEVPWCDIRPEFGASCTIVWRYLKQAGIELSADLATAFLYGIKTET
                     RDLGRECGPHERTAYLELSAQANHTKLYSITNPKLSREHFVAMDRACRRAICWGELLA
                     VNLGTVQYPDLVAEIAELMLAYEKARYCLCLGEYEGQVFLSIRSEDEDSHAGELIRRV
                     VGSKGAAGGHGMSAGGRLHQTVRSETELKILYDELVARLVAELAINQPPVPLL"
     gene            7404..7817
                     /locus_tag="PKL17_11740"
     CDS             7404..7817
                     /locus_tag="PKL17_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45451.1"
                     /translation="MSNANPWNRPLPHLAVRVASVFFRRPRGLRWLGKGMAGLVALLL
                     LLADGGISGDELQCELAADHILTCCPELTALPLSCVRSGCGGQMVPSLDETRAACLRA
                     AGCEQLRAMGVCDPNGWEAPMGCASPCNTKVPRCQ"
     gene            7808..8452
                     /locus_tag="PKL17_11745"
     CDS             7808..8452
                     /locus_tag="PKL17_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45452.1"
                     /translation="MPIKDTRLLRVSAFAIGLWGWLPSSQAVGTPAGSPPAASHDGVS
                     EGAEAAPPIVVEVQPSPRRARLFGRLTAGFVYRWALQQSFIGGSFEGELGARDHRFAA
                     GGRLRVEAGKMLAGLPYQVITIGPSFWLPPLAERIHFGMGLEGGALLIDRRTVPGSAM
                     WTVMWGGRVDAVLDLVRMGDSGAVYFATGVSAQALTTAPFPVTVATGFAIGYRP"
     gene            complement(8494..10578)
                     /locus_tag="PKL17_11750"
     CDS             complement(8494..10578)
                     /locus_tag="PKL17_11750"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK45453.1"
                     /translation="MSMTTPRLRSDLRSEPVEDAQGVLYFDVSDPKNGGVLRLFDFEW
                     LLALKFDGQRGLDEIAKQAEGELGLAASADDLKVYAERLGQLGLLEGVMAQPGKLPGN
                     LMATAYLQAAGLVARVEVTPYTGKAAAAPAVEKPVEKPVEKLAPPVKTPDVVAAKPAP
                     VVEPQKPVAPVKPAEPVVVAKPIEPVRRTPAREDDDRMGQPVLQLRPPSRVAAESPKP
                     ALSEPPAKPTPPVKVAPPVETVAPPVAAPRSNLLEALESLPPPVDEPEAASTPAKIEL
                     PPSVTETPSSLLNEVMAATKPEPVVEAKKAPVVPIEVELPPKPVEAPKPVEVAPPVEV
                     AKPVEPPKPVEVAKPVEPPKPVEPQKPAMVQPTPPAQEPSGGGGKWVVIVLLLLAIGG
                     AVFWFVVKPMMTKVSVKTAPALLADVTSNFSTPAKVKASEPTVLKLGAAGKVQKLVDE
                     GKSVTAGETLVELDSFAAIGKAQVDAQKALDGLKKKLETGKLKGKAAADLQAKITEKQ
                     NKIAELETQGKAARLVAEKDGAVAKVLVKQGQAVTAGTDAVSVADKGLVAELQVPAND
                     SASLSGGQTIALCNTGKTLSIKATIKSVTKQDSQNVVQIALPADAAVKAGDELLVEKG
                     KIEQVASLPATAVVDGKVFIVKDGKVATVAVAVAGQESSSVLVKGLTGGEQVIQARSP
                     ELHDGVEVEIAR"
     gene            complement(10581..11174)
                     /locus_tag="PKL17_11755"
     CDS             complement(10581..11174)
                     /locus_tag="PKL17_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNK45454.1"
                     /translation="MSFINYSSREINCKLVYYGPGLCGKTTNLQYIYEKTNPEAKGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYEASRRLILKSVDGVVFV
                     ADSQEARFEANIESLEDLRSNLVEAGYNPDKMPFVIQYNKRDMPNVMPVEDLRRELNP
                     NNVPDFEAIAVKGDGVFETLKAVAKLVLTELKGQKKG"
     gene            complement(11369..11860)
                     /locus_tag="PKL17_11760"
     CDS             complement(11369..11860)
                     /locus_tag="PKL17_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNK45455.1"
                     /translation="MEMLFEEEQRKIQAISDRLQRDSNSIAVLVINKDGQEIARSGQT
                     KDLDITSLSSLFAGNVAATSAIAKLLKEEEFGGQFHEGTKYNVNISVVAKRAILAVLF
                     DSNSSIGLVRLRVRRSSEEMTKVFDDLVHKIQQQQKGGGGGHGPNLAGEITDDDIDKL
                     FGE"
     gene            complement(11990..12601)
                     /gene="recR"
                     /locus_tag="PKL17_11765"
     CDS             complement(11990..12601)
                     /gene="recR"
                     /locus_tag="PKL17_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNK45456.1"
                     /translation="MLSGTSSGDPIQRLIRELSKLPGIGQKTATRLAFFVLRSPLEQA
                     QGLAKALVEVKERIRLCSVCMNLTESDPCELCSDPSRESRLLCVVAHPPDLLSIERTS
                     SFRGRYHVLHGVLSPLQGIGPDDLRIRELLNRVTPPSPVEEVILATSPNVEGEATALY
                     LSRALKAVGVRVSRIASGLPIGGDLEYADGITIARAIEVRRDM"
     gene            complement(12620..16843)
                     /locus_tag="PKL17_11770"
     CDS             complement(12620..16843)
                     /locus_tag="PKL17_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45457.1"
                     /translation="MRPKTCSFLPVRIVGGVSALIGLLATMSCSTQTVTTPTRSLDRP
                     SDIALFCVEFDSKDSGCLPSLDPAADPQAYLDAFCGNTGWRSTTPTATVLPRTECDEG
                     HRRLRRDSYFQGPRKAAQLLGRNPDTPCCAADDTACTAVAPTCMYRSELALVVNTVRG
                     EISVANTALQTPGLSTYGRLANLHGGKPGFGFLPAGNLPLHVRATVPVPPGEAASTLD
                     GQFAWAVSSNAGSCDFSVVALQPVANLVARAPSCDDEGQTCATRDCDGQSCPKAIQPW
                     VPSGAGGKKFLSARPGWIEIAPWSRASERRIVAAFPTCGIIATIDVSDSPNSGRIYEA
                     VAFDQKDAKNLPKVLTAAELAGLKCPADCGGDSSPLPPDAMDIPPGGAMKSASYPASL
                     AVDTYGSRILVSDGLGDGVTLIDYDAAAADGSHLLASLRRVTLDFEVRSDGRTAQKGL
                     DAIRISPRTPAGQFAYVVARDSSVRVIDLDREVECETNPDARYLLSQAASSLRVLPDE
                     FVESNLRRLSCLPVDKTKTPRAPLALGPGLLLPNGSMPRDIGLTHVDTVPCTSTDPNQ
                     CAYSENPDPSAYVPSSAALWVGDFAWLLGGAGTVLGVQIADRCPSPSYRACFPESAAL
                     RRLALLHTRSQALTNPEQLGLPAQPQALTVQPQDRLGNVRRLVPRFDERADTGPSGPR
                     PDSDATGIPVQTARIGGSLVSTYTTLTDAATTLTRRRLLLPTLTPYYYLPVDPVCDVA
                     IAEQAMTLTSPDQNLPVEPTRRPVTTVAFTDPASAASESWTMAWEGVLQGLGRTTGLL
                     QPDGTLLDLNGLYCSRGVETGDKLWLTGCYSNSDCPSGYLCKREQTQISSPGLCMTRD
                     QETQCRALSEMLVTDSSNDTVWAASWLRRYLISKAEQQITATPPLSDVIDRLTLDELP
                     EPEFVIERQLCSVSDLGKSDVCSDPLFAPPVTSRANAPGVACRVTGRDSMGKPSASCI
                     RQCEVNTDCGTGFVCAHSHYEADEKAALGATPKARCVRAPLIAEGTPWNVGGTWRAMT
                     SKDAAEVKSACFPDQVRYEVHGGDSMVIRGDRTPFATLVRRGSDGVCRRPQPGDGTFA
                     ASRTLQPRISLGPHASVADGDPRRCPSVQQGLIGHRIPASADAVGAPSCQNLWKNGGT
                     VVLPYGQGDFPLHLVRGQDFYPQPPDLKTERQGQVLGHPRGGWQWDPSCVKNPMDPRC
                     ASDIKRAAGDPWLNREFELFSMLPLDGKLNQCILTSPTEEVYPTNGAEQVGCSGFCRF
                     PGDHTEIQGVRRIHYENALGNLVLRVPRRLVNTDLPLDTRPTITDMTGKTISNPSYNP
                     IVWAVPPEGYGVVFSVVGGAQPFVQFAQTAQRDASSGILAQGLRAAATGLDGTVYLVD
                     EGRSGSATGLRGQIMRLVGSIIDPYFLLR"
     gene            complement(16846..>17905)
                     /locus_tag="PKL17_11775"
     CDS             complement(16846..>17905)
                     /locus_tag="PKL17_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45458.1"
                     /translation="DRGCVEPGYRHDRGTFLSDKNQNPSGRPPCYKLDGAKVLPGHYY
                     QYLVSSHLPTGQVSSFDLGQSPISLATPLLGDVSGSLITTTGGQTGAFAIAPRKAGDL
                     SQPWYVTSKLTSTLATFRLGVGPKVVPGLLFGLSGQFSTASQDVRSIVFERSGDRAYL
                     ALFQPPALAVVDTRLRGGGSSIPVNQVSAVVNLCAGPSRQVIAKVPRYSMGQVVMTTR
                     VFVTCYLSGQVAEIDPDTGELIGTVQAGRGPLSIALNFGSDETATAVGYSGAIDPCAD
                     PLSTATDATRVGVTCPGTGADIRPRPFGAGQSPLPPRAFVSNYLDNTISVIDLDPRSP
                     SYRRMVARIGLPSPKQVQ"
BASE COUNT         3420 a         5332 c         5591 g         3562 t
ORIGIN      
        1 gtccaaagtc gacaatcttc accacatcct gtctcccctc ccgctcgagg agaaagatgt
       61 tgtccggctt caggtcgcga tggacgatgc ccttgtcgtg gacggcttgg aggccgcggg
      121 cgatttgggc accgattcga cagacgcgca gtggcggaat cggtcccgat gtgatggctt
      181 cggtgagtgt ctcgccttcg agaaactcca tgaccaagta gggatgcccc tcgatcatgc
      241 caaagtcggt gaggtcgacg acgttggtgt ggttgatcat cgacgcggtc tgggcttctt
      301 ggatgaagcg cttccacgcc gattcttctt gggctttgcg caggactttg atggcgacgg
      361 gcttgcgtag ggtgacgtga cgggcccggt agacgacgcc cattccgcct tcacccagcc
      421 gtccctcgac ggtgtagcgg ttgtcgatgg tcgtgccgat cagcttgtcc tctgtgagcg
      481 tcgacggatc ggcgagcggg cagatatgca ccgtcccggt cttgtacgtc gcaccgcagc
      541 gagaacacgt cgctgaatcg gcgttatctg ggcttgccgt gctcacagtc gttgctgcgc
      601 ccttctgcat cctcgtggcc ccgacgcaac accggcagtc gcagctcgcc gtcgagagtg
      661 agagatgaga gcgtcagaat aggtccatga tacacaaaga caatttgtaa tgaaaagaac
      721 aatagaggcc ggggtaattg gcaaaaaaga gtgaaaaagc gatggaattg cgcttatttc
      781 cgtcagaagt aatgccatgt ggttttgtac atacaatagc tatttataaa actaattttc
      841 ctcgatgaga tatcatggat gcgggtatat gctgcgccat cctctgcgcc cgacgagtag
      901 cgggccctcc ggcgcgcatt gccaagcagg gcgagcacaa tgatcgggac gcgagtaggt
      961 ccataccgga tcgaacggaa gcttggtcaa ggcggcatgg gcgcagtcta cgaggcggtc
     1021 cacgagacca tcgagcgtcg tgtcgccatc aaggttctga acccccaact ggcgcaaaac
     1081 tcagacgttt cggtccggtt catcaacgag gcgcgtgccg tcaacttggt gaaccatcca
     1141 gggcttgtgc aggtatcgga cttcgggcag atgcccgacg gaaccgccta catcgtcatg
     1201 gagctgctcg aaggcgagac actgagcaag cggctcgcgc ggctcggggg caagctgccg
     1261 ctgccagaaa tgctcaagct ggtgcggcaa atctcgtcgg cgctccaagc cgcccacatg
     1321 aaaggcatcg tccaccgaga tttgaaaccg gacaatgtaa tgctggttac ggaatctgat
     1381 ccggaaactg gcacgcggga gcgggtgaag ctgctcgact ttggcattgc caagatgtcg
     1441 gcttcgtcgc agggggaaaa caaatcgaac acccagaccg atgcggtgct gggttctccc
     1501 gaatatatgt cgccggagca gtgcaagggc ggcgtcaaca tcgaccagaa agcggacctg
     1561 tattcgcttg gcgtgatgat gtaccgcatg ctgtcggggc ggctgccgtt caacgccgag
     1621 ggtgttggcg cgctgatggc gatgcacatc tacgaaaagc cgccggatct gaggccgctt
     1681 gcgccggata cgccgccgga tttgatcgcg cttgtcgaca aaatgctgtc gaaagttccg
     1741 gcagagcgac cgaccgccgc gcaggttcaa tacgaaatcg agcagctggc aacgcgcgtc
     1801 ggcatgccga tggtcacaag ctcgatgcag gcgatccgct cgcagatcat gacggcggag
     1861 tcgatgccgg agtcggtgcg tggcatctcg acgctgggcg gggcgtcggg tcaggccgcg
     1921 gtgccggcac caccgcccaa gtcaaaagcg ccgctgtttg tcggtttggg cgtgggtttg
     1981 ctcgttttgg tcggtgcggt ggtcggtgcg ctgtcgctgc gcaagcctga caagccacag
     2041 gcccaggtgg tgatgcagca aaaacagcca gatccaccga aggaaacgcc gccgccgccg
     2101 cccgaaccca agaagcgacc gaagtggacg gtcgacagcg agccgcaggg cgccgacatc
     2161 attcggaccg acgatggaca gctgctcggg cagacgccgt ggacgggtga gcagcccgat
     2221 ggttccggtc ctgtcgaggt gacgatccat ctgacggggt tttcggatcg caaaattacg
     2281 ctgaagccgg gcccggacac ttcctacaaa cagacgttga agccaaaacg gggccgcaag
     2341 ggcaaagctg ggaaaaacca ggatcaaaag aatgatggac cagaaatcgc ggactaacgc
     2401 tctttcgtcg tggcgaagtg gtctctgtgc gctggcgctc ttggccgttt cgtcgccggc
     2461 tgtcgctgct gagcagagca gtggcgatga tggcgtcgca gcgaccgatc cgtgcctgac
     2521 cgacaatctg tgcaaaggcc actacgacga agcgcgcaaa gcctacaaag acaagcacta
     2581 cgacgaggcc ttcaaggaat acaagtcggc gtatgagcga cggcaggctc cgtggctgct
     2641 tatcaacatt ggtcgttgtt cgcagcggct tggacgcaac aaggaagcgc tcgattacta
     2701 ccagcgctat ctgaaggcgg atcccaatcc aaagcccgag acggttacgc gcgtaaatgg
     2761 ctacatcgac caggtcaagg ccggcatcat cgacgagccc gaagcgaagc cggaaccgaa
     2821 gcccgaagcg aagccggaac cgaagcccga acctcagccg cagctgacga cggcgcagca
     2881 gaacacccaa gccaccgcgg agccggaaaa gacgccggtc tacaagaagt ggtggttctg
     2941 gacgatcatc ggcgttgtcg tcgcaggtgg cgtcgcaggc ggtgtcgcag gcggtgtgat
     3001 tgccagcaaa aacgcaaccc cgcaagttgt atcaaaccca cctgtcccgt cgggtgtcac
     3061 cgtttatatg ccttcctttt aaccaattta ccgcttgagg aggaatccat gaaaaatcga
     3121 tttgtcagga atggaagccg gctctgtctt ttggccggtg cattgcttgc aagttcttgc
     3181 agcccctcga cggttccaga cagtcagatt ctgattgtgg tcaatgtgaa gggcatccag
     3241 agcggcaccc aagcgctcca ggtcaagtcg tctctcgacg gacgagctga tccaaatggc
     3301 ctgacgatta ccaacaacac cagcaaattt gcgattcagc tcgcaaacga tccgaaaaac
     3361 tacgggcagc tcaagctcga cgggtacgcg ctggacaaca accagtgcta cctggccaac
     3421 ggtcaagtca ccgagcagat cactgcgcaa aagacgtact acgagctgga ccttacgctt
     3481 ttgccgcagg cgtcgccgaa gtgcagcttg acggtcaaca ttctgagcac cggcaccgag
     3541 acggtcacgt cgtccccggc agggatcaac tgcggcggtg gccagacgac ctgttcttac
     3601 gattttccct ttgggacggc agtcacgctg agcggtccgg tgagccaggc atcgtatccg
     3661 gtctggacgg gcggctgcac gcctgcggcg acgtattact cagcaacgtg cgttgccaac
     3721 gtgcagaagg gcaacgccag cgcgacggtc gacttcgttc cgcgggtctg ctctccggat
     3781 ggttggtgtc agtatcaccc gctgggaacg acgcagacgc tcatcgacgt gtggggcgcc
     3841 gacgccaaga atctgtggat cgcggggacc agtggcacgg tgttgagctc gtcaggcggc
     3901 gcctggctgc cgtcggcgaa cccgtggggt acaaaccagg tttcgtttgt ccgcgggacc
     3961 aacgcgagca acgtctatgc gtcaggatcg agcaccacga ccagcctgat gcgctgggac
     4021 ggcaccaagt ggtctgctca gcccgcactg ccgaaccaga tcaacaccaa ccttctgggt
     4081 atgcatgtga tcggttcgag cgacgtgatg ctgagcgtcg cggactacac cagtgcgccg
     4141 accaagtacc tgatttatcg cttcaacgga acgacgtata gcagcatcgg tcccacgctc
     4201 ggggccagcg agtacctgac gaggctgtgg ggcttcggcg gcaatgacgt gtgggcgggc
     4261 agctcgcttg gcatctacca ctttgatggc accaagtgga ccaaagatac gagcactgcg
     4321 attcagaaca agatcatcca gttcatttgg gggtcggcga ccaacgacgt ctgggccgcg
     4381 acaactgcgg agctgtttca ctgggatggt acgacctgga cagccgcagc gccggacaat
     4441 ggcgtaaccc caacgaacat cttgggaatc ggcggtggca ctgggccgga tgtctgggtg
     4501 acgacgtccg ctgcaaacgg tcgtttctat cgctacgcag gtggcagctg cagtcccaag
     4561 tgctggtcgc aagtcgatgt cacgggggtc acgacgctga tccgctccgt ctggggagct
     4621 accaacaatg acctgtgggc cgtcggaaac accggcctta tcctgcacta cgacggccaa
     4681 acctggagcc gctcgcagta tcagaaagtg ccgctgacga cgggagcttt gtacgctgcc
     4741 tatggcgttg ccaacaatca aacgtcggcg atgcaagcct acggaccgac tgacaccgcg
     4801 ctcaatgtcg acaacacgaa cattgtgcca gcgaacggat tctcaacggc cacacagtcg
     4861 atttttgcgg cctatgggct caatgccaac gaaatcatga tcgttggctc gggtggtacg
     4921 ggcggtggca cggtgatgcg ctgggatgga accagctgga agacctatcc atcgggggcg
     4981 ccgaactttg cctatttgtg gggcgtctac atcaacaaca gcccacggta ctactacatc
     5041 gtcggtgaac agggctatat cgcgcgcgct gatgcaaccc tgactggctt tacgccgatg
     5101 acgaagaccg tggctacctc ggcgtcattt tatgcggtcg gtgggtcgag ctatcaaaac
     5161 ctctacgcgg tcggctcggg tggcaccgtg tatcgctcga cggacgggat gaacttcagc
     5221 ccggtgactg ccccaaacaa cgataccggg cagacctact attcggcctt ttaccatcct
     5281 ggctcggggc gtgtgtacat cgcagggacc aacggcaacc tgtggtacta caccggggca
     5341 ccctgggccc aggtcaatac ggcatcggca gcgacgcttt acgggatgtg ggggatgtct
     5401 ggctcgcagg cgctgtggct cgctgggagc aacggtacca tcctgaagtt cgacggcacc
     5461 aacgtcgctg cgctcaaaac cgggaccacg tctacgctgc gttcgatctg gggcaacaac
     5521 ttgacggaca tttgggccgt cggtctctct ggcacgattc tgcgctggaa gatgtaatcc
     5581 gactggctct tttggcttgg ggcccaaaga gccgcccgcg ctgctacttc gacagccgcg
     5641 tattcagcag ttcactgagc gccaagaggg catcaatgac cgtgaagatg cccaccacct
     5701 tgccgttctg ttccacaatc gccgatccat actttttcgc cgccatctct tcgaccactt
     5761 catcgagctg ggactcaggc gagatcacat agacctcttg gctcatcgcg tcttcgaccc
     5821 ggacattgtt cggatcgacg tccttgagcg tttcaatcag gtgaatgtct cggatcgaaa
     5881 ccagacccac gagcgatccg ccactgagca ccggcaggtg acggatgttg tgctcgcgca
     5941 tgatctttgc cgcttcggcc agcgacatgt ctgggcgaac ggtgtgcggg ctggtcgtca
     6001 tgtacttctg aactttggga atcggcttgc tcatcggagt ttctctcttt ggtctccttc
     6061 gggagacacc gttaagttgt gaatccgatg ccacttcgta aagagctgaa cgtctgtcta
     6121 gaaacgcagc aatgacgcca gcttccgtcg agcagcgagc agacgcgcga gctcaatatg
     6181 aatcatttca caggaataat attggcagcg ccgcgccaga tcgacgaagc gacggaatgg
     6241 ttacagcagc ggtacaggcg gttggttgat ggccagctcg gcgaccagtc gcgccacaag
     6301 ctcgtcgtac agaatcttca gctcggtttc gctgcgcaca gtttggtgca gcctgccgcc
     6361 ggcgctcatg ccgtgacccc cggcggcgcc tttgctcccg acgacgcggc ggatcagttc
     6421 accggcatgc gagtcttcgt cttcgctgcg aatcgacaaa aagacctggc cttcgtattc
     6481 gcccagacac aagcagtagc gcgctttttc atacgccagc ataagctctg caatctcagc
     6541 gacgagatcc ggatactgca ccgttcccag attgaccgcc aaaagctcgc cccagcagat
     6601 ggctcgacgg cacgcgcggt ccatcgcgac aaaatgctcg cggcttagct tgggattggt
     6661 aatcgaatac agcttggtgt gattggcctg cgccgacagc tccagatagg ccgtgcgttc
     6721 gtgcggtccg cactcgcgtc ccaggtcgcg tgtctcggtc ttgatgccgt acagaaacgc
     6781 cgtcgccaag tccgccgaca gttcaatgcc tgcttgcttc aaatagcgcc aaacaatcgt
     6841 gcatgacgcg ccaaactcgg gccgaatgtc gcaccacgga acttccgccg aaatcggcct
     6901 tggcgggtgg tggtcaatca cgatgtcggg gagccggcct gccggcagcg agttgttgcc
     6961 cgtgcccggt tgggtatcga gcagcgcgac aatcccaaac gagtccaagt cgaggttttc
     7021 gagcgggcgc agatcgaacg acagcacccg taccatggcg cgattttcgg cgcggccaat
     7081 gattccggcc atgccgatgg tgcattcaat cccttgccgt cccaaaagca gcgacaagcc
     7141 cgccgctgcc gccagcgaat cggggtccgg attgtcgtga ggcaggagca gcgcccgcgg
     7201 tctgcgcgtc tttgcccatt gtcgcgccat gaccagggca ctgccgagat caaacaccat
     7261 gtgacatcac ggtatcgcgg ctgcctggcg gaagcaagcg cggagccgac gctgcggtga
     7321 cgtgaacttg ggaatcgggg cggacgcttt ctgcccgatg cttttcttgg cctggcccga
     7381 cgtggcatgc tgcgcagtac gccatgtcga acgccaaccc ttggaatcgc ccgctgcccc
     7441 acttggctgt gcgggtcgct tctgtctttt ttcgtcgccc acgcggtctg cgctggcttg
     7501 gcaaagggat ggctggcttg gtcgcgctgc tgcttttgct cgccgatggg ggcatttccg
     7561 gcgatgaact tcagtgcgag ctggcggcgg atcacatctt gacctgttgt ccggaactga
     7621 cggcgctgcc gctatcgtgc gtgcgttccg gttgtggcgg tcagatggtg ccgagccttg
     7681 atgaaacccg cgccgcctgt ctgcgtgccg ccggctgcga gcagctgcgt gcgatgggtg
     7741 tctgtgatcc aaatggctgg gaagcgccga tgggctgtgc ctcgccttgc aacaccaagg
     7801 tgccgcgatg ccaataaaag acactcgctt gctgcgcgtt tccgcgtttg ccattggact
     7861 ctggggttgg cttccatcct cacaagcggt cgggacaccg gctggatcac cgcccgcagc
     7921 gagccacgat ggcgtgagtg aaggcgcgga agctgccccg ccgattgttg tggaagtgca
     7981 gcccagtcct cgtcgggccc ggctgtttgg tcgactgacc gccggctttg tttatcgctg
     8041 ggcactgcaa cagtctttca tcggcggatc gtttgaaggc gagctgggcg cgcgcgatca
     8101 tcgctttgcg gcggggggtc ggctgcgggt cgaagcgggc aaaatgctcg ctggcttgcc
     8161 gtatcaagtg atcacaatcg gtccaagctt ctggcttccg ccgctcgccg agcggattca
     8221 ttttggcatg ggactcgaag gtggcgcgct gcttatcgac cgacgcactg tgcccggttc
     8281 tgcgatgtgg actgtgatgt ggggcgggcg ggttgatgcg gtgctcgatt tggtccgcat
     8341 gggggattct ggcgcagtgt actttgccac gggtgtttcg gcgcaagcgc tgacgaccgc
     8401 gccgtttccg gtgacggttg ccaccgggtt tgctatcggc tatcggccct aagctggaat
     8461 gggatttgcc gcagcagaag tgctgcgtgc ggactagcgg gcgatttcga cttcgacgcc
     8521 gtcgtgaagc tcgggcgagc gcgcttggat gacttgctca ccaccggtca ggcctttgac
     8581 aaggacgctg ctcgattctt ggccggccac ggcgaccgcc acggttgcga ccttgccgtc
     8641 cttgacgatg aacaccttgc catccaccac ggcggtggct ggcagcgacg cgacttgttc
     8701 gattttgcct ttttccacca gcagttcgtc accggccttc accgcggcat ccgccggcag
     8761 ggcgatctgc accacgttct ggctgtcctg cttggtgacc gacttgatgg tcgctttgat
     8821 ggatagcgtc ttgcccgtgt tgcagagcgc gatggtctgg ccgccgctca ggctggcgct
     8881 gtcattggcc ggcacttgca gctcggcgac aagacccttg tcagcgacgg atactgcatc
     8941 ggttccggcg gtgactgctt gaccctgttt caccagcact ttggcgaccg cgccgtcttt
     9001 ttccgcgacc aggcgtgcgg ccttgccctg ggtctctagc tcggcgattt tgttctgctt
     9061 ttcggtgatt ttggcttgga ggtcagccgc ggccttgccc ttcagcttgc ccgtctcgag
     9121 tttttttttg agaccatcga gcgctttctg cgcatcgact tgggccttgc cgatcgcggc
     9181 aaagctatcc agttcgacca gcgtttcgcc cgccgtgacc gactttcctt cgtcgacgag
     9241 cttctgcact ttgccggcag cgccgagctt gagaacggtt ggctcgctcg ccttcacctt
     9301 ggccggcgtc gaaaagttgc tcgttacgtc ggccagcagc gctggagccg tcttcaccga
     9361 aactttggtc atcatcggct tgacgacgaa ccagaacact gcgccaccga tggcgaggag
     9421 gagcaggaca atcaccaccc acttgccgcc accaccgctc ggctcttgtg cagggggcgt
     9481 cggctgtacc atcgctggct tttgcggctc gacgggcttt ggtggctcga caggcttcgc
     9541 aacctcgacg ggctttggtg gctcgacggg ctttgcaacc tcgacaggcg gcgcaacctc
     9601 gacgggcttc ggcgcttcca ccggctttgg cggcagctcg acctcgatgg gcaccacggg
     9661 cgccttcttg gcctcgacga ccggctcggg cttggtcgcc gccatcactt cgtttagcag
     9721 cgagctgggc gtctcggtaa cagacggtgg cagctcgatc ttcgctggcg tagaggcggc
     9781 ttccggctca tcaactggcg gcggcagcga ctccagtgct tccagcagat tggagcgcgg
     9841 cgcagcgact ggcggcgcga cggtttcgac gggcggtgca accttgaccg gcggcgtcgg
     9901 cttggctggc ggctcgctga gcgccggctt cggcgactct gcggcaaccc gcgacggcgg
     9961 acgcagctgc aacactggct ggcccatgcg gtcgtcgtct tccctggcgg gcgtgcgacg
    10021 gaccggctcg atgggctttg ccacgaccac cggctcggca ggtttgacgg gggcgaccgg
    10081 cttttgcggc tcgaccaccg gggctggttt ggcggccacg acgtcgggcg tcttgacagg
    10141 cggtgccagc ttttcgaccg gcttttcgac tggcttttcg actgctggtg ccgccgcagc
    10201 tttgccggtg tagggcgtca cttcgacgcg agcaaccaag cctgccgcct gaagatacgc
    10261 agtggccatc aggttgccag gaagcttgcc cggctgggcc atcacgcctt ccagcagtcc
    10321 gagctggccg agacgttccg catagacctt cagatcgtcc gcgctggctg caagtccgag
    10381 ctcgccttcc gcctgcttgg cgatctcgtc caggccgcgc tgtccgtcaa acttcagggc
    10441 cagcagccac tcgaaatcga acaaccgcag gaccccaccg ttcttcgggt cgctcacatc
    10501 aaaatagagc acaccctgcg cgtcctcgac gggttcactg cgcagatcgg agcgaagtct
    10561 gggcgttgtc atcgacatgg ctaacccttc ttttgaccct ttagctcggt cagcaccagc
    10621 ttggccacgg ccttgagcgt ctcgaacacc ccgtctcctt tgaccgcgat ggcttcgaag
    10681 tctgggacgt tgttcgggtt cagctcgcga cgcagatcct cgactggcat cacgtttggc
    10741 atgtcccgtt tgttgtactg aatcacaaac ggcatcttgt cggggttata tcccgcttcg
    10801 acaaggttgc tgcggagatc ctcaagcgat tcgatattgg cctcgaagcg cgcttcttgg
    10861 gaatcggcca cgaaaacgac gccgtcgacg ctttttagga tcaggcggcg gctggcttcg
    10921 tagaacacct gaccaggaac ggtatacagg tggaagcggg tcttgaatcc gcgaatctct
    10981 ccaagtgaaa gcgggagaaa atcgaagaac agcgttcgtt ccgtttccgt tgccaaagag
    11041 atcatcttgc ctttggcttc ggggttcgtt ttttcataga tatattggag attggtggtt
    11101 ttcccgcaga ggcctggtcc gtaatagacc agtttgcagt tgatctcgcg tgagctgtaa
    11161 ttaataaagc tcatcgtata tatcctgaat cccgcttatt tgcgaatcct ggctgcctgt
    11221 gcagataccg ctgaacattg ctgcgcaata cgtctggata tgccagggac ttcccgcgaa
    11281 ccaggaagaa tccagatgca gccaactcac aagtgtgcca attacaacac tcggaggtca
    11341 gcggagggaa cccactgcgg agcacgagtt actctccaaa cagcttgtcg atgtcgtcgt
    11401 cggtgatctc gcccgctagg ttcggaccat gtccaccgcc gccgcctttc tgctgctgct
    11461 ggattttgtg caccaggtca tcgaagacct tggtcatctc ctcggatgag cgacgaacgc
    11521 gaagccggac gaggccgatc gacgaattcg agtcgaacag caccgccagg attgcgcgtt
    11581 tggcaaccac ggagatattg acattgtatt ttgtgccttc gtggaactga ccgccaaact
    11641 cctcttcttt gagaagcttt gcgatagccg aggtcgctgc cacattgccg gcaaacagcg
    11701 aagacagcga cgtaatgtcg aggtctttcg tttgaccaga acgggcaatt tcctgaccgt
    11761 ctttgttaat cacgagcact gctatcgagt tcgagtcgcg ctggaggcgg tcggagattg
    11821 cctgaatctt ccgctgctcc tcctcgaaca acatttccat ggcgaccggt tctcctcgcg
    11881 tgggaccctt agcgcctgct tcaccgagag cagacgggct gagcccaaaa gatagtgagt
    11941 tcccttttat caggctttaa agatggttgc aagcattacc atcctgcggc tacatgtccc
    12001 gccgtacctc gatcgccctg gcgatggtga tgccgtccgc atactcgagg tcgccgccaa
    12061 tgggaagacc ggacgcgatc cgtgacaccc gcaccccgac cgccttgagc gcccgagaca
    12121 aatagagcgc cgtggcttcg ccttcgacgt tggggctcgt cgccaagatg acttcttcca
    12181 ctggcgatgg cggcgtgacc cggtttagca gctcgcggat gcgcaggtcg tcgggaccaa
    12241 tgccttgcag cggggacagc acgccatgca gcacatggta tcggccacgg aaactgctcg
    12301 tccgttcgat cgataacagg tccggcggat gggcgaccac gcaaagcaag cgggattccc
    12361 ggctcggatc gctgcacagc tcgcacgggt cgctttcggt caggttcatg cagaccgagc
    12421 agagccggat gcgttctttg acctcgacga gcgctttggc caagccctgc gcctgctcca
    12481 aaggtgagcg caggacaaaa aacgccagcc gagtcgcggt cttttggccg atcccaggta
    12541 gcttggacag ctcgcggatg agacgctgga tcggatcgcc gctcgaagtg ccgcttagca
    12601 cggagtacct cgtcgctggt tagcggagca ggaagtatgg atcgatgatg ctgcccacga
    12661 gccgcatgat ctgaccgcga aggcccgtcg ctgaaccgct gcggccttca tcaaccaggt
    12721 agaccgtccc gtcaagtccc gtcgccgccg cgcgcaagcc ctgcgccaaa atgccgctcg
    12781 atgcatcgcg ctgggcggtc tgggcaaact gaacgaatgg ctgggcgcca ccgacgacgg
    12841 aaaagacgac gccgtagcct tccggcggca ccgcccagac gatcgggttg tagcttgggt
    12901 tgctgatcgt cttgccggtc atgtcggtga tcgttgggcg cgtatccagc ggcagatcgg
    12961 tgttcaccaa tcgacgcggc acccgcagca ccaaattacc gagcgcattt tcgtaatgga
    13021 tacgacgaac gccttggatt tcggtgtggt cgcctgggaa acggcagaag cccgagcagc
    13081 caacctgttc agcaccgttt gtcggataga cttcttccgt cgggctggtc aagatgcact
    13141 ggttcagctt gccgtcgagc ggcagcatcg aaaacagctc aaactcacga ttgagccagg
    13201 ggtctccggc ggcccgtttg atgtcggatg cacagcgcgg gtccatcggg tttttcacgc
    13261 agctcgggtc ccactgccaa ccgccgcgtg gatggccaag cacctgtcct tggcgttcgg
    13321 tcttcagatc aggcggctgt ggatagaaat cctgtccgcg taccaggtgc agcggaaagt
    13381 cgccctgtcc ataaggtagg acgacagtgc caccgttttt ccataagttt tggcagctcg
    13441 gtgcgcccac ggcatccgcg gaagccggga ttcggtgccc gataagtcct tgttgcacgg
    13501 atggacagcg gcgcgggtcg ccatcggcaa cgctcgcgtg tggcccaagc gagatgcgcg
    13561 gctgcaacgt tcggcttgcc gcaaacgtgc cgtcaccggg ctgcggacgg cggcagactc
    13621 cgtccgaacc gcgtcgaacc agcgtcgcaa atggcgtgcg atcgcctcgg atgaccatcg
    13681 aatccccgcc gtggacttcg tagcgcactt gatcggggaa gcatgcagac ttgacctccg
    13741 ccgcatcttt gctggtcatt gcgcgccacg ttccaccgac gttccatggt gtaccttcgg
    13801 cgatgagcgg cgcacgcacg cagcgggcct tgggggtcgc gccgagggct gctttttcgt
    13861 ctgcttcata gtgcgaatgg gcacagacaa agccggttcc gcagtcggtg ttgacttcac
    13921 actggcgaat gcagctggcg cttggcttgc ccatgctgtc gcgaccggtg acgcggcagg
    13981 ccacgcccgg cgcattggcg cgcgaagtga ccggtggggc aaacagtgga tcgctgcaca
    14041 catccgactt gcccagatcg ctgaccgagc agagctgccg ctcgatgaca aactccggtt
    14101 cgggtagttc gtcgagcgtg agtcgatcaa tgacgtcgct aagcggtggt gtcgcggtga
    14161 tctgctgttc tgccttgctg atgaggtagc gacgcagcca gcttgcggcc cagacggtgt
    14221 cgttgctgct gtcggtcacg agcatttcgg acagggcgcg gcactgcgtt tcctgatccc
    14281 gcgtcatgca cagaccgggt gagctgatct gcgtttgttc gcgcttgcag aggtaaccgc
    14341 ttggacagtc agagttgctg taacaaccgg tgagccacag cttgtcgccg gtctcgacgc
    14401 cacggctgca ataaagaccg tttagatcga gcagtgttcc gtctggttgc aaaagccccg
    14461 tcgtgcgacc tagcccttga agcacgccct cccaagccat ggtccagctt tccgatgcag
    14521 ccgacgccgg gtcagtgaat gcgaccgttg tgaccggtcg ccgggttggc tcgacgggca
    14581 ggttttgatc cgggctcgtc agcgtcatcg cttgttcggc gatcgcgacg tcacagaccg
    14641 ggtccaccgg caggtaataa tacggcgtca gcgtcggcaa cagcaggcgt cgccgggtca
    14701 acgtggttgc ggcatcggtc agcgtcgtat acgtgctcac cagcgatcca ccgattcgag
    14761 cggtttggac ggggatgccc gtcgcatcgc tatcgggacg tgggccgctc ggtccggtgt
    14821 cggcgcgttc atcgaagcgt ggaaccagcc gccgcacatt gccgagtcga tcctgcggct
    14881 gcaccgtcag cgcttgcggc tgggcgggca ggccgagctg ttcgggattg gtcagcgcct
    14941 gcgatcgggt gtgcagcaga gccaggcgac gcagcgccgc agactcgggg aaacaggcgc
    15001 gataggacgg gcttggacag cgatcggcga tctggacacc aagcacagtg ccggcaccgc
    15061 cgagaagcca tgcaaaatcg ccgacccaca gcgccgcgct cgacggaacg taagccgatg
    15121 gatcgggatt ttcggaatac gcgcactggt tcggatcggt cgacgtacat ggcaccgtgt
    15181 cgacgtgggt gagcccgata tcgcgcggca tgctgccatt tggcaataga aggcccggac
    15241 ccaaggccaa cggcgcccgc ggtgtcttgg ttttgtcgac gggcagacac gacaggcgac
    15301 ggaggttcga ttcgacaaac tcatcaggca gcacgcgcag gctcgacgcc gcttggctca
    15361 gcagatagcg agcgtcggga ttggtctcgc actcgacttc ccgatccagg tcgatcaccc
    15421 ggacgctgct gtcgcgggca acaacataag cgaactgacc cgccggcgtg cgcggcgaga
    15481 tgcggatcgc atcaaggcct ttttgcgcgg ttcgtccgtc gctgcggact tcaaaatcga
    15541 gagtgacgcg acgcaggctt gccaaaaggt gtgaaccatc cgctgctgct gcgtcgtagt
    15601 ctatcagcgt tacgccatcg cccagaccgt cgctgaccag aatgcgcgat ccataagtat
    15661 cgacggcaag agacgccgga tagctcgccg atttcatcgc gccgcccggt ggaatgtcca
    15721 tcgcatcggg cggtagcggc gaactgtcgc caccacagtc agcggggcac ttgagaccgg
    15781 ccagctctgc cgccgtcagg actttgggca ggttcttggc gtctttttga tcgaacgcga
    15841 cggcttcata aatccgaccg ctgttcgggg aatccgaaac atcgatggtg gcgatgatgc
    15901 cgcaggttgg aaacgcagcg acgatacggc gttcacttgc gcgcgaccac ggggcgattt
    15961 cgatccagcc cggacgcgcc gaaaggaatt ttttgccgcc agcgcctgac ggaacccacg
    16021 gctggattgc tttggggcat gactggccgt cgcagtcgcg ggttgcgcag gtctgaccct
    16081 catcgtcaca cgacggagcc cgggccacca agttggccac gggctgcagc gctacgacgg
    16141 aaaaatcaca cgaaccggcg ttgctgctga cggcccaggc aaactgtcca tccagcgtcg
    16201 atgctgcttc acccggtgga actggaaccg tcgcacggac atgcagcggg aggtttccgg
    16261 cgggcagaaa gccaaagccg ggtttgccac cgtgcaggtt ggcaagccgt ccatacgtcg
    16321 atagtccggg ggtctgaagc gcggtgtttg cgacggaaat ttcgccacgg acggtgttta
    16381 ctaccagcgc cagctcgctt cggtacatgc aagtcggagc gacagcggtg caggcggtgt
    16441 catcggcggc gcagcacggc gtatcgggat tccgcccgag aagctgcgcc gctttgcgtg
    16501 ggccttggaa atagctgtcg cgacggagcc gacggtgtcc ttcgtcgcac tcggttcgcg
    16561 gaagcacggt tgccgtcggc gtcgtcgagc gccagccggt gttgccacaa aacgcatcga
    16621 gatatgcctg cggatcagcc gcaggatcga gggatggcag gcagccgctg tctttggagt
    16681 cgaactcaac gcagaacagc gcaatgtcgc tcgggcgatc gagcgagcgc gtcggagtcg
    16741 tgaccgtctg ggtgctacag ctcatcgtcg ccaaaagccc gatgagggcg ctgacaccac
    16801 cgacgatacg cacaggcagg aatgagcaag tcttgggtcg cacagctatt gcacctgctt
    16861 gggggacgga agtccgatcc gggcaaccat tcgtctgtag cttgggctgc gcgggtcgag
    16921 atcaatcacc gaaatggtgt tatcaaggta gttgctgacg aaagcacgcg gcggcagggg
    16981 ggactggccg gctccaaacg gacgcgggcg aatgtccgcg ccggtgccgg ggcaggtcac
    17041 accgacgcgg gttgcatcgg ttgcggtgct gagcggatcg gcgcacggat cgatggcgcc
    17101 gctgtagcca actgccgtcg cggtttcgtc tgatccgaag ttcagcgcga tgctcagcgg
    17161 tccgcgcccg gcctgcacag tgccgatgag ctcaccggta tcggggtcga tttccgcgac
    17221 ttggccagat aggtaacaag tgacgaacac ccgcgtcgtc atcacgactt gacccatgct
    17281 gtagcgcggg actttggcga tgacctgacg agacggaccg gcgcacaggt tgaccaccgc
    17341 cgacacttgg tttaccggaa tgctgctgcc accgccgcgc agacgggtat cgacgacggc
    17401 gagcgctggc ggctggaaca gcgccaaata cgcgcgatcg ccactgcgct caaacacaat
    17461 ggatcgaacg tcttgcgatg ccgtcgaaaa ctggcccgaa agaccgaaca gaagacccgg
    17521 aaccactttc ggaccgacgc ccagtcggaa ggtcgccagc gtcgatgtca gcttcgaggt
    17581 gacgtaccac ggctggctca gatcgccggc tttgcggggc gcaatcgcaa acgctccggt
    17641 ttgtccaccg gtcgtcgtaa tcagcgaacc actgacatcc ccgagcagcg gcgtcgccag
    17701 cgatattgga ctctgtccaa gatcaaacga tgacacctga cccgtcggca gatggctcga
    17761 taccaaatat tggtagtagt gaccgggaag cacctttgct ccgtcgagct tgtagcaagg
    17821 cggtcggcca ctcggatttt ggtttttgtc cgacaggaaa gtaccgcgat cgtggcgata
    17881 gccgggttcg acgcagccgc gatcg
//
LOCUS       DAORGN010000095        17866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1276_length_17866_cov_6.698445, whole genome shotgun sequence.
ACCESSION   DAORGN010000095 DAORGN010000000
VERSION     DAORGN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 17866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17866
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_17866_cov_6.698445"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="PKL17_11780"
     CDS             <1..1498
                     /locus_tag="PKL17_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNK45459.1"
                     /translation="RPGQPGANTKPGAGTGGTAIAGTRPAAGKPALGQKPTGGTQAKP
                     PQPAVVQNKPSTQQPSESADARPQQFGRPSYVPGTGVYIPSEGDPRRAGVPAAQPGKR
                     QEVGEAVWLPPQARKTPPGGPREVLLEQSPRPQGALLNFDIHNGYVLAMAGGSDYDRS
                     EFNRVIQACRQPGSAYKPIYYSLALDRGYSYDTMWNDKVKVEIDPVTGEQWLPTNIDG
                     SYGQQVNLERALIWSKNPPALEVFKTMGKKDVEGWARRLGMTTPIHADDALALGASCV
                     RMDELAKSFALFGRSGRPVDLVTVKRVVSRTGEVLVDNSAWDDPMLDGKTRLERVIAQ
                     AGRQKRPIIAPRTAYLTGTLLRRVVSQGHSKPIRDVGIMAAGKTGTSSRTSDVWFVGF
                     TSRWLTSVWIGDDTYERQLGFADASFTLSVPMWARYVYHANRDAPLSEVPERPASVKP
                     TDRGGPLILGFPPPPSAGIRPDGTPYELPENLRDLPTAPKGTRPPKIF"
     gene            1736..2392
                     /locus_tag="PKL17_11785"
     CDS             1736..2392
                     /locus_tag="PKL17_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK45460.1"
                     /translation="MSLMKTLADAFHEGGWPMWPILLLMIIAWGISIERVIYLKKAGI
                     DKKKLLSLLKSQIMAGNIQGAVKVCSGNPTPVTRIIQAGLTKFNRPDAEVQAAMDEQA
                     LHEIPKLEARTGYLAMLGNVATLVGLLGTILGLIHSFAGVAGVDPSMKATLLAKGISE
                     AMNCTAFGLVCAVPALLWFAILNGWTQHTLDDINEVSVQVVNLVVGHRNAMKNAGGQG
                     "
     gene            2462..2944
                     /locus_tag="PKL17_11790"
     CDS             2462..2944
                     /locus_tag="PKL17_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK45461.1"
                     /translation="MGGAAPTPSGKGGKKPLDAAINLVPFIDLLSCCISFLLITAVWT
                     QLARMDVTQKSKSQGAPNDDPQPNQVTLTLFINKDNTFTFSKSTGESIAIEAKADDYD
                     YVKLADTLAQAKKDHADKEDLTIKTDDVVVYHKIIKAMDIAISAKFPTVTLTDKGAGG
                     "
     gene            2947..3534
                     /locus_tag="PKL17_11795"
     CDS             2947..3534
                     /locus_tag="PKL17_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK45462.1"
                     /translation="MSSAFDKGGLIKQAGPRLRKFIPLKFVTKHGAGKKSTYAELNLT
                     SMVDMLTILVVFLLQTFSASGELLTVSKNVTLPEAMNYKDLEQAPIISISRDSVTLNG
                     EPKADASELARETTMDWKIPQLHDDLVVLKNNFKLLHPDPNDWKGLVIVQGDKMIDFK
                     VLKKVMYTCTVAGYNNVNFAVQQKAKGGGGGGEHK"
     gene            3756..5423
                     /locus_tag="PKL17_11800"
     CDS             3756..5423
                     /locus_tag="PKL17_11800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45463.1"
                     /translation="MLRRSRNLWAHPTALGFALTLSTFTLTGCPEDPDDPKSWVSKLD
                     NPREQKEALRQLARLKEKAEPAYEALAAKYKKTPDTEVLRTIAKLRTNKAVDLFAEQL
                     DYSEETYDNAIVAANGLQDIAERDDKGREAAKGAVAALKKAAGKKLSVRSRANVARIE
                     SIKALAAIKDPSSVDTLNEILETSADDQDFFINKTTAKVLAEFADPKSIKSLTRGLWM
                     TGRGNDMFQECRLGLVRIGEPAVDKLVETLQRKNADVEADAKKYKFVPGIIVQKAAIL
                     LGDLRSKKAVPALLAELQKKDDGLGTGPDGMPGVSGHQAILQSLGQIGDPSATKAVLA
                     VLSDGKRDPKHRAAAAEGLNMLGDMSALPILLGIAKTPFLTPAKNKDEMPELDTNKAT
                     LVAYAVTQASRLTDKDESAVLEPIYKTIPADIVDIKKYFETAMARTKVAAECKKDVAC
                     YDKYLGMPPDADAKVENSPKAERAAFSLARLGHDAVPVLKKYVGHKDTAVRSAINFAL
                     TKTATKADADVLKALAEQIDKDKTRDKTGQALAEEARIISAIITNRS"
     gene            complement(5474..6622)
                     /locus_tag="PKL17_11805"
     CDS             complement(5474..6622)
                     /locus_tag="PKL17_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK45464.1"
                     /translation="MTTFFREEHELFRKSVSQYLEKEIVPHVEEWEKAELFPREVFKR
                     CGELGFLGAHYPEDVGGAGGDWWYAAAWGESLVKSRCAGLAMALMVQTDMATPIIAEI
                     GTREQKDEFLAPALRGEKIAALGVSEPNCGSDVASIRTTARRVGGDFIINGAKCWITN
                     GTRADFITLAVRTGPEGSGFGGISLVTFPTDVKGFKVTRKIKKMGNHASDTAELSFED
                     CKIPARYLLGEENHGFYYIMMNFQGERLIAAVSAVAGAQQCLDETIQYTQDRAAFGRP
                     ILKFQVWRHTFAELATQIEAARWLSYRAVDLFNRKEEAVKEITMAKLFAGELAIKVAD
                     RCVQAHGGYGYAEEFPIARYYRDVRLITIGGGTSEVMKEILSKRMGWG"
     gene            complement(6736..7120)
                     /gene="rnpB"
                     /locus_tag="PKL17_11810"
     ncRNA           complement(6736..7120)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKL17_11810"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7194..8012
                     /locus_tag="PKL17_11815"
     CDS             7194..8012
                     /locus_tag="PKL17_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45465.1"
                     /translation="MAQPTQQSTAIAVLKVMFFIACSIGALGLFDLAVSRQLWQVSQA
                     LWMLRHLPERQLADLREGPIVLRGTLEASHVRLSPGGEPSVLYYGWIEDHYRSGRSDV
                     VRVLCNVGEDEDLRFRQGDHTIPFEVFHHEEHIALLKRSGLFESLPMERVGIDLGRVH
                     SQKVKLGQLPEGLAERCPTDMSPRGTLYYKEARLPVGQPVVVLGCPKDGVLRSCASES
                     PVPGMLAAQSHARLLSAYAEAPMNWIRGSCVLVAFALGYLCSLLLKSSSSEVST"
     gene            8009..8224
                     /locus_tag="PKL17_11820"
     CDS             8009..8224
                     /locus_tag="PKL17_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45466.1"
                     /translation="MIQSWVDVIITFVVCWIGLHYYFEARKRRSARSQKGQRDIAASV
                     VMWLAAILSASALTVLTTSLVLAWIVR"
     gene            complement(8476..9111)
                     /locus_tag="PKL17_11825"
     CDS             complement(8476..9111)
                     /locus_tag="PKL17_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45467.1"
                     /translation="MPRLSDATLRSRLLSLPKTEFDHSAIDSLPLAAQAVLWAQTQLA
                     ESEAGPSSGRLPPALVSSATALRQIMIEVCAYHFRHDVRLQAQIDDIRGGQGYLDLAE
                     DLRRLAALYRSEHKTLSADTRFYQKSDADQALHFAQRIVSELRPVRSDAAREQVFRTF
                     ALLLKLYEEVARAARFLLRESGDTAFPSLYAAGRSPSRRKTSSRSPKPTPE"
     gene            9367..9873
                     /locus_tag="PKL17_11830"
     CDS             9367..9873
                     /locus_tag="PKL17_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNK45468.1"
                     /translation="MSQKTSKEHLLTAEEIANGPELALSHPLNREKSEVHIRPLGARV
                     GLARTQVSMGRIPPGKESFIFHRHLADEEWVYILSGRGEAKIGDETFAVSAGSFMGFP
                     TPSLGHSLMNTAEEDLVYLMGGERSRLEVAEFPTAGKHAIFSAEGIFLIDSQQLQRMG
                     FEDYVKKG"
     gene            complement(9942..11783)
                     /locus_tag="PKL17_11835"
     CDS             complement(9942..11783)
                     /locus_tag="PKL17_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45469.1"
                     /translation="MNKLIGKSRLHIASWLVALALGGGCSDNTNESMTLPPDIIPPDG
                     DPCSLAPCQNGGVCHYDGATASCECPSGFTGQFCEQNINDCPANACENGGTCVDGVNS
                     YSCACPAGTAGEHCEIDLDLRVTMPRRMVSDKSLTLRAEMLDPATGKIVTDGCFDTLG
                     SVTIKRADDGSDVPLTVTVFDDHLPVPDDSIRFYHGVGSVSLTLDGGAAVPAGEYLVT
                     VTVGDRTATRHLTVESNPTWRVMPANLSGADLTWGPNENIRLSQHNTTVPAGSTLTIL
                     PGTMIMVDTTGKIEDGTLLTVNGKITATGTFDQPIHFFSERGAQAMTHVISGSSLSNP
                     NAWRGIFLYGGDSSNLDWVILTGAGNGTVVSHPRPPIINIAGTHNLAVSDSIFTDATG
                     MVFQSPSTGTHSIRRSLVSRVGIGAEFLSSGNKVDIEDSWWTSIGRGPTSPLRYDGDG
                     IHVDGAASRQTIRRCYVVDIGDDAIDHSNSTFTIEDTVIHDAGDKAVSMTNGSATVRN
                     SLIYRTGSGVRGTAKVYNSTILSPSPIANPLVLSDSIIWPNTLSSCSGTFTNNILGTG
                     SDLSCGTGNISVDPMFNNAAMCDYSLAVNSPALTASSTGGVLGYVKK"
     gene            12393..14405
                     /locus_tag="PKL17_11840"
     CDS             12393..14405
                     /locus_tag="PKL17_11840"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNK45470.1"
                     /translation="MGTDPAPPDMAQPVDMAQLDTARLLLLRSARWPGGGREMTVGVL
                     NQTTGLAFGSDLSTRLTALLPAGISLTSKVQKMALPPGYTAILLPPTLSATERADLAS
                     AIVRFANQRPASERIALYRHGATVQLFSNYLIERTKLTEALDRYQRGANGDQNPLPLV
                     QAVGPAASDVENIGGSGPDVMRSLVILNPNPQVVFTGLPRVFVIAVTPDSAGLAAASA
                     AIDTNRQNAFYKFAACSIPDKFSAKFQVTDMLGELEASFPATLPEELGAACNVDNIDT
                     AKRVYTPKIELVFDATQRAAHDARIKAAEVAPYDDVLARSDFETQVRLAPGQPTVLAT
                     AHLHGNSSIRCPRHSYIISLTGSDRYLLPDSASDEYTLISMCNDPAYVYAPTAFRLFT
                     DDIFPLKFRFVEFAIDGNTRGIYLLMEKTKEELIRDNARVTSVMRRDYPQGTTDFFEV
                     LYSDTPDLNDPINRWKFFAARIAALSGDDLVSALRSQLDLDQYLRYIAAQSVLKSGDY
                     IDEVFFYGAEQANGAGGVTETYRMMAWDPEGYSTCHAGGVNAYPDPNQLSYCSEGKLD
                     WKILADQKVYYLLVKKIEDALNTTLTREKMLASLTQTKTELNALFSTPAVCAAMTELL
                     KINAGAADCTVARSVISARADAILAAYDARRAALLPLIASYRNKYP"
     gene            complement(14464..14856)
                     /locus_tag="PKL17_11845"
     CDS             complement(14464..14856)
                     /locus_tag="PKL17_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45471.1"
                     /translation="MRKNLDRSVLTAKTWTFGLGATCGLLLALGTWSCATLGIGQSGP
                     PQQKGEPTLSKCMTGSTLEKHTSCAEYCASQNLGCQNYGCAVPGDKNRYGGAAFDNTL
                     CAGNMMKGFQCNEPLLGVGAVACCCTGL"
     gene            14965..15450
                     /locus_tag="PKL17_11850"
     CDS             14965..15450
                     /locus_tag="PKL17_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNK45472.1"
                     /translation="MILQALLLAVSEGAEEKAPPVIDIDGTVLVQFAIFIAMFIVLRQ
                     FLFKPYMQMREDRAHRISSAETAAAETQKRAAQLEKDFKEKMDKAKQSAEDERLKLSH
                     EGRQREEELLSSARERAQGRIRATREQVGAQVAQAQTELSQRAEALSKVLAAKLLGRE
                     V"
     gene            15455..16480
                     /locus_tag="PKL17_11855"
     CDS             15455..16480
                     /locus_tag="PKL17_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45473.1"
                     /translation="MKTRTSIARLSLGLLVLSASTLASAQVPEAPAVNKPEGIAKPAP
                     IHNPSGQYPPLRQAGQAAAPQAAQPAAQPEAAAPAQPATADAVPAAQPTEHPAAAPPA
                     PTEPAHAEPAHAEPAHPAGSEHAPPHGEAATADHGAAHGEHAAAHGEHGGAHEAGPVI
                     ENWWSWDYGPGKTHHHPPFGFALINFAVFLLILNKLAGKSFKEFLANRHLEVRKALDR
                     ARELEKQAQDQLAEYEKKSQAMTAEVDALLKSMKEQAQIERQKIIAKAEAEAEKLLKD
                     AEAQVQQTIEGAKRDLERKTALLAVDLAEKLVATRLSDMDQRNLVERFVTQVETQSTH
                     TATGGRL"
     gene            16477..17016
                     /gene="atpH"
                     /locus_tag="PKL17_11860"
     CDS             16477..17016
                     /gene="atpH"
                     /locus_tag="PKL17_11860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNK45474.1"
                     /translation="MISGSASRRYARAILGIGQSQDNLESLTAEVERLASAIEHSADL
                     RTVLLNPAFTQTQRQDVLGTVCQRLALSKTSQNLALLLLSRGRVANLPGIARSLRQMV
                     DEVAGRVRVRLTSARPLDAAAETRIRSALGKATGKTVLVEKNVDESLIGGVVTQVGDV
                     VYDGSLRGELTSLRARWQG"
     gene            17107..>17866
                     /locus_tag="PKL17_11865"
     CDS             17107..>17866
                     /locus_tag="PKL17_11865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNK45475.1"
                     /translation="MELRAEEISQIIRKQIEDYDQKVSVQETGTVLMAGDGIARVYGL
                     SGAMAGETLDFPHGIKGMVLNLEEDNVGVALLGNYELIREGDVVKRTGQIASVPVGKG
                     LLGRVVNALGQPVDGKGPLQSTESRKVEVKAPGIVYRKSVHEPMQTGIKAIDAMIPVG
                     RGQRELIIGDRQTGKTAVAIDTIINQKGQNMFCIYVAIGQKQSTVASVVDKLTQTGAM
                     EYTCVVVAGASEPAPLQFLAPYTGVTIGEYFRDNG"
BASE COUNT         3774 a         5500 c         5271 g         3321 t
ORIGIN      
        1 ccggcccgga cagcctggag caaacaccaa acctggtgct ggaacgggag gcacggcaat
       61 cgcaggaacg cgacccgccg ctggcaagcc cgcacttggt caaaagccca ccggcggaac
      121 ccaggcaaag ccgccgcaac cagccgtcgt ccagaacaaa ccgtcgaccc agcagccaag
      181 cgagtccgcc gacgccaggc cgcagcagtt tggtcgacca agctacgtcc ccggcaccgg
      241 cgtttacatt cccagcgaag gtgatccgcg tcgcgccggc gtcccagcgg cccagccagg
      301 aaagcggcag gaagtcggcg aagcggtgtg gctgcctccg caagcgcgca agaccccacc
      361 gggcggtcca cgggaagtgc tgctggaaca aagcccgcgt ccgcaaggcg cgctgctgaa
      421 cttcgacatc cacaatggtt acgtgctcgc gatggccggc ggcagcgact acgaccgatc
      481 ggagtttaac cgcgtcatcc aagcgtgccg gcagccgggc agcgcctaca aaccgatcta
      541 ctactcgctc gcgctcgacc gtggctacag ctacgacacg atgtggaatg acaaagtgaa
      601 ggtcgaaata gacccagtca ccggcgagca gtggctcccg accaacatcg acggcagcta
      661 cggacagcag gtcaacctcg agcgggcgct tatctggtcg aaaaatccgc cggcgctcga
      721 agtgttcaag acgatgggca aaaaggatgt cgaaggctgg gcccggcggc tcgggatgac
      781 gacaccaatc cacgccgacg acgcgctcgc gctcggggca tcgtgcgtgc gaatggatga
      841 actggcgaaa agctttgccc tgtttggccg cagcggtcgc cccgtcgatt tggtaacggt
      901 caagcgcgtg gtgtcacgga ctggcgaagt ccttgtcgac aacagcgcct gggatgatcc
      961 catgctcgac gggaaaacca gacttgagcg ggtgattgcg caagccggca gacagaagcg
     1021 cccgatcatc gccccccgca ccgcatacct gactggcacg ctgctgcgcc gggtggtttc
     1081 gcaaggtcac agcaagccga ttcgcgacgt cgggatcatg gccgccggca aaaccgggac
     1141 ttccagccgc acgtccgatg tgtggttcgt tggttttacc tcgcgctggc tgacttcggt
     1201 gtggattggc gatgacacat acgaacgcca gctgggattt gctgacgcca gcttcacgct
     1261 cagcgttccg atgtgggcac gctacgtcta tcacgccaac cgcgacgcgc ctctgtccga
     1321 agttcccgag cgcccggcga gcgtcaaacc gaccgatcgc ggcggtccgc tgattttggg
     1381 tttcccaccg cccccaagcg ctggcattcg acccgatggc acgccctacg agctgcccga
     1441 aaatctgcgc gatctcccga ccgcccccaa agggacccgc ccacccaaga ttttttagcc
     1501 gcgcaggctg cgaccgtttc tgacccagcc cactgccaga accccagcgc caaaccgtgg
     1561 catttttccc cgatcgtgtt gcgcatttcc ccgcttctca aaggccccaa aacgcctgcg
     1621 aggcctgccg tttcgcgaac ttccggggaa ctgcaaattt ttctatccgc tggcactcgg
     1681 tgtgcttgta ccatgggcca acacttcaaa cccgttccac tggaggtact ggacaatgtc
     1741 gttgatgaaa actctggccg acgcgttcca cgagggtggc tggccgatgt ggccgatcct
     1801 tctcctcatg atcattgcgt ggggcatctc gatcgagcgc gttatctacc tgaagaaggc
     1861 aggcatcgat aagaagaagc tgctttcgct cctcaagagc cagatcatgg ccggcaacat
     1921 tcagggcgcc gtcaaggtgt gcagtggcaa cccgaccccg gttacccgca tcatccaggc
     1981 tggtctcacc aagttcaacc gtcccgacgc tgaagttcag gccgcgatgg acgagcaggc
     2041 tctccacgag attccgaagc tggaagcccg caccggctac ctcgccatgc ttggcaacgt
     2101 cgcgaccctc gtcggtctgc tcggcacgat tctcggtctc atccactcgt tcgcaggcgt
     2161 cgccggtgtc gatccctcga tgaaagccac cctgctcgcc aagggtattt cggaagcaat
     2221 gaactgcacc gcattcggtc tggtctgcgc agttccggcc cttctgtggt tcgccatcct
     2281 gaacggctgg acccagcaca ccctcgacga catcaacgag gtgtcggtcc aggttgtgaa
     2341 cctggtggtc ggccaccgca acgcgatgaa gaatgcgggc ggtcagggct aatctcgacg
     2401 gtgacgggtt ggggcggatc gaagtgtccg cccttatgag cccaagcaag gagtaattac
     2461 gatgggcggc gcagcaccta cgccatctgg caaaggcggt aaaaaaccgc ttgatgccgc
     2521 aatcaacctg gtcccgttca tcgacctttt gtcgtgttgt atctcgttcc ttctgatcac
     2581 cgctgtgtgg acccaattgg cccgcatgga cgtgacgcag aagagcaaat cgcagggtgc
     2641 tccgaacgac gatccacagc cgaatcaggt cacgctgacc ttgtttatta acaaggacaa
     2701 tacgttcacg ttttcgaagt ccaccggcga gagcatcgca atcgaagcca aagcggatga
     2761 ctacgactac gtgaagctgg cagatacctt ggcccaggcg aagaaggacc acgccgacaa
     2821 ggaagatctg acgatcaaaa ccgacgacgt ggtggtctat cacaagatca tcaaggcgat
     2881 ggacatcgcc atctcggcga agtttccaac tgtcactctc acggacaagg gagcgggagg
     2941 ctaaacatgt ccagcgcatt tgacaagggt ggccttatca aacaagccgg cccgcggctc
     3001 cgcaagttca tcccgctgaa gttcgtgacg aagcacggcg ccgggaagaa gtcgacctac
     3061 gcagagctga acctgacctc gatggtcgac atgctcacga ttctggtcgt cttccttctc
     3121 cagaccttct cggcatctgg tgagctcctc accgtgtcga aaaacgtcac gcttcccgaa
     3181 gcgatgaact acaaggatct ggagcaagcg ccgatcatct cgatttcgcg cgactcggtg
     3241 accctcaacg gtgaaccgaa ggccgatgcg agcgagctgg cgcgcgaaac gacgatggac
     3301 tggaagattc cccagcttca cgacgatctg gtggtgctga agaacaactt caagctgctg
     3361 caccccgatc cgaacgactg gaagggtctg gtcatcgtcc agggcgacaa gatgatcgac
     3421 ttcaaggtgc tcaagaaggt catgtatacg tgcaccgtcg ctggctacaa caacgtcaac
     3481 ttcgccgttc agcagaaggc gaagggtggc ggtggcggcg gcgaacacaa gtagaccgat
     3541 tcctgagccc gactcctctc ttcccttctc tccttccgat tctcccgact caaaccgata
     3601 caaaatccgc cgtgaaaatc acggtggacg agttcttgcc ttttgggtcg acccagatgc
     3661 attctgtttg ggcggcgtgt ggtacgccgc gacggcgcgc tacggatgcg ccttattgct
     3721 ttgtgttctg agcacgcccg cgcgaggagt tcgccatgct gagacggagc cgcaacctgt
     3781 gggcacaccc aactgccctc ggctttgccc tgaccctgag cacgttcacc ctaaccggct
     3841 gtcccgagga tcccgacgat cccaagagct gggtcagcaa gctcgacaac ccgcgtgagc
     3901 agaaagaagc gctgcgtcag ctcgcccgcc tgaaggaaaa agcggagccg gcttacgaag
     3961 cgctcgcggc gaaatacaaa aagacgccgg ataccgaagt gctgcgcacc attgcgaagc
     4021 tgcggaccaa caaggcggtc gatctgttcg ctgagcagct cgactactcg gaagagacct
     4081 acgacaacgc gatcgtcgct gcgaacggtc tgcaagacat cgccgagcgc gatgacaaag
     4141 gccgcgaagc tgccaaaggt gccgtcgctg cgctgaagaa ggccgccggc aagaagctgt
     4201 cggttcgttc acgcgccaac gtcgcccgca tcgaatcgat caaagcactt gcggcgatca
     4261 aagacccgtc gtctgtcgat acgctcaacg aaatcctcga gacgagcgct gacgatcagg
     4321 acttctttat caacaagacg acggcgaaag tgctcgctga gtttgccgat ccgaagtcga
     4381 tcaagtcgct tacgcgcggc ctgtggatga cgggccgtgg caacgacatg ttccaagagt
     4441 gccgactcgg actcgtgcgc attggtgaac cggcggtcga caagctcgtc gaaacgctcc
     4501 agcgcaagaa cgccgacgtc gaagccgacg ccaagaagta caagtttgtc cccggcatca
     4561 tcgtgcaaaa agccgcgatt ctgctgggcg acctgcgcag caaaaaggcg gttccggcgc
     4621 tgctcgccga gcttcagaag aaggacgacg gacttggtac gggtcccgat ggcatgcccg
     4681 gcgtctctgg acatcaggcg atccttcagt cgcttggtca gattggcgat ccgtcagcga
     4741 ccaaggctgt gctggcagtg ctaagcgatg gcaagcgcga tccgaagcac cgtgcggcgg
     4801 ctgccgaagg cctgaacatg ctcggcgaca tgagtgcgct gccgatcctc ttggggattg
     4861 cgaagacgcc gtttttgacg ccggccaaga acaaagacga gatgccggag ctggacacca
     4921 acaaggccac gctggtcgct tacgcggtca cgcaggcctc gcggctcacc gacaaggatg
     4981 agtcggcggt gctggagccg atctacaaga cgatcccggc ggacatcgtc gatatcaaga
     5041 agtacttcga gacggcgatg gcccgtacca aagtcgccgc tgagtgcaaa aaagacgtcg
     5101 cgtgctacga caagtacctg ggaatgccgc cggacgccga cgcgaaggtc gaaaacagcc
     5161 cgaaggcaga gcgtgcagcg ttctcgctgg cgcggctcgg acacgacgca gtgccggtgc
     5221 tcaagaagta cgtgggccac aaagacacag cggtgcgctc ggcaattaac tttgcgctga
     5281 ccaagactgc gacgaaggct gatgccgatg tgctcaaagc gctggccgag cagatcgaca
     5341 aagacaagac tcgcgacaag acgggtcaag cgcttgccga agaagcccgc atcatctctg
     5401 cgatcatcac gaaccgctcg taagctcccg gcccccctcg ctggggtcgg gcgcttccgc
     5461 ccatctttcc gaatcagccc cagcccatcc gcttcgatag aatttccttc atcacttcgc
     5521 tcgtgccgcc accgatggtg ataagccgca cgtcgcggta gtagcgagcg atcggaaact
     5581 cttcggcata gccgtagcca ccgtgcgctt ggacgcagcg gtcggcgacc tttatcgcca
     5641 gctccccggc gaacagcttg gccatcgtga tctcttttac cgcctcttcc ttgcggttaa
     5701 acaaatccac ggcgcgatag ctgagccagc gagcagcctc aatttgcgtc gccagttctg
     5761 cgaacgtgtg acgccagacc tggaacttca ggatgggccg accaaacgct gcgcgatcct
     5821 gggtgtactg gatcgtttcg tccaggcact gctgcgcccc ggcgaccgcg ctgaccgccg
     5881 caatgagccg ctcgccctgg aagttcatca tgatgtagta aaagccgtgg ttttcttcgc
     5941 cgaggaggta gcgcgccggg atcttgcagt cctcaaacga cagctcggcg gtgtcggaag
     6001 catggttgcc catcttcttg atcttgcgcg tgaccttgaa acccttcaca tccgtcggga
     6061 acgtcacaag cgagatgccg ccaaagcccg aaccttccgg cccagtgcgc accgccaggg
     6121 tgatgaagtc ggcgcgggtg ccgttggtga tccagcactt ggcgccgttg atgatgaagt
     6181 cgccgccgac gcgccgtgcc gtggtgcgaa tcgacgccac atcactgccg cagtttggtt
     6241 ccgacacgcc aagcgctgcg atcttttcgc cacgcagcgc cggggccaaa aactcatctt
     6301 tctgctcacg ggtgccaatc tcggcgatga tcggcgttgc catatcggtc tgcaccatca
     6361 gcgccatcgc caggcccgca cagcggctct tgaccagcga ctcgccccag gccgccgcat
     6421 accaccagtc accgcccgca ccaccgacat cttcgggata gtgcgccccc agaaagccca
     6481 gctcgccgca gcgcttgaac acttcgcgcg gaaacagctc ggctttttcc cactcctcga
     6541 cgtggggcac gatctctttt tcgagatact gggagacgga cttgcggaat agctcgtgtt
     6601 cctctcggaa gaatgtcgtc agcatgcggc aatcaaagca aaacccccgc ccccgagcaa
     6661 gccccgtgca gctttgaaaa acgacggcag atcgatgatg acccaagtcg cacgtcggaa
     6721 tcggaagcag tgttctcgtc ggagcggacg gtaagccggg ttctgttcgt cccttgcggg
     6781 ccgtagcggt cattcatcta ggcccacctt tgcagatggg ctctggcgac ctacctgaag
     6841 ttcttcttgc gaagcgcgac gggccgccgc ggatgcttgc gcatcgactt ctatgcggtc
     6901 ttgctttgga tggggttttc cctgcggccc ctgtcaccag gcgcccggtg cgctcttacc
     6961 gcaccttttc acccttacca gccgaggctg gcggtttgtt ttctgtggca ctttcctgca
     7021 agtcaccttg actggccgtt agccagcatc ctgccctgta aagcccggac tttcctcccg
     7081 cctgccttgc ggcaagccag cgaccacctc atccactccg acgagaaccg ggcgaagcta
     7141 gcgcagaatc aagggctcgt tcaagaatca ttgctaaaga gcgcgcaaaa ccgatggcac
     7201 agccaaccca gcaaagcaca gcgattgccg tgctcaaggt gatgtttttc attgcctgtt
     7261 ctatcggcgc gctggggctt ttcgacctcg cggtgagccg tcagctgtgg caggtcagcc
     7321 aagcgctgtg gatgctgcgt cacctgcccg aacgccagct tgccgacctt cgcgaggggc
     7381 cgattgtttt gcgcgggaca ctcgaagcaa gccatgtacg gctctcgcca ggtggtgagc
     7441 catcggtttt gtactacggc tggatcgaag accactatcg ttccgggcga agcgacgtgg
     7501 tgcgcgtgct ctgcaacgtc ggcgaagatg aagacctgcg ctttcgccaa ggcgaccaca
     7561 ccatcccgtt cgaggtcttc catcacgaag agcacatcgc actgcttaag cggagtggcc
     7621 tgttcgagtc cttgccgatg gaacgggttg gcattgacct gggtcgtgtc cattcgcaaa
     7681 aagtcaagct cggccagctt ccggaagggc tcgccgaacg ctgccccact gacatgagcc
     7741 cgcgcggcac gctgtattac aaagaggcgc gtcttcccgt aggtcaacct gtcgtggtgc
     7801 tgggctgtcc caaagacggc gtgctgcgca gctgtgcaag cgaatccccg gtgcctggca
     7861 tgctggctgc gcagagccac gcccggcttc tgtctgccta tgccgaagcc cccatgaact
     7921 ggattcgtgg cagctgcgtg cttgtggcgt ttgcgctcgg ctacctgtgc tctcttctgc
     7981 tcaagtcgag cagcagcgag gtctcgacat gatccagtcc tgggtggatg taatcatcac
     8041 ctttgtcgtc tgctggatcg gtcttcacta ctacttcgaa gcccgcaagc gtcgcagcgc
     8101 ccgcagtcaa aagggccaac gcgacatcgc cgcctccgtc gtcatgtggc tagccgccat
     8161 cctctctgcc tccgccctca ccgtcctcac cacaagcctc gtcctcgcgt ggattgtgcg
     8221 gtagcggcca aagccaagat cgaccttggt caaaggccgc atgtcaggcc gtttgccaag
     8281 gtgaaccttg gcgcaacgcc gacttcccgg tcacatttca acatccacct tggtcacagg
     8341 ccctttaccc ggtcacattt caacatccac cttggcaaac agccgatgca tcggtgtttc
     8401 gtcaaggtgc accttggcaa acagcccgct tgtgggccat ggaacaagat gcaccttggt
     8461 attgcggcga tgtgatcatt ctggcgtcgg tttcggggaa cgagaggaag tctttcggcg
     8521 tgatgggcta cgccctgcgg cgtacaaact cggaaaagcg gtgtcgccgg attctcggag
     8581 gaggaaacgg gcggcgcggg cgacttcttc gtagagcttg agaagaagcg caaaggttcg
     8641 gaagacctgc tcgcgcgcgg catcggaacg aacggggcgt agctcgctga cgatgcgctg
     8701 ggcaaagtgc agcgcttggt cggcatcgga tttttggtaa aagcgggtat ctgccgatag
     8761 cgtcttgtgc tcgctgcggt acagcgcggc cagacgccgc aggtcttccg ccagatccag
     8821 atagccctgc ccgcctcgta tgtcatcgat ctgcgcttgc agccgcacgt catggcggaa
     8881 gtgataggcg cagacctcga tcatgatctg gcgcagcgct gttgctgagc tgacaagcgc
     8941 aggtggaagc ctgccggaag aaggccccgc ctccgattcg gcaagctggg tctgtgccca
     9001 cagcacagct tgcgccgcaa gcggcaggga atcgatcgcg ctgtgatcaa attctgtctt
     9061 gggcagcgac aaaagacggc tgcgaagtgt ggcgtcagaa aggcgcggca tggtgtcaga
     9121 caacacaaag ctggcggcgg cacgcacgtc ggcgcgcggg gggccgatgc gttctgtggg
     9181 aagcgaaaga agtgcggagc gcaaggcctc aaaggcggcc tcacccggat cgatgtgaac
     9241 ggcggcagga ggggttttgg atttggttgg gagggaggac tttttcttgg gtggttgcat
     9301 ctcggaatga gaactcgacc tgctaggttc cgcaagggca tctgcaaaaa aaatcaagga
     9361 gttgtcatga gccaaaagac ctcgaaagaa cacctgctga cggcagaaga gatcgcaaac
     9421 ggacccgagc ttgcgctaag ccatccgcta aaccgcgaaa agtcggaagt tcacataaga
     9481 ccgcttggcg cgcgcgtggg tcttgcgcgg acgcaggttt cgatgggacg gattccgccg
     9541 ggcaaggaat cgtttatttt ccatcgccat ctggccgacg aggaatgggt ctacattctg
     9601 tctggccgcg gtgaagcaaa gatcggagac gaaacctttg ctgtgtccgc tggcagcttc
     9661 atgggattcc ccacgccgtc tttgggacac agcttgatga acaccgcgga agaagacctg
     9721 gtgtacttga tgggagggga acggagtcgg ctagaagttg ccgagtttcc gacggcaggg
     9781 aagcatgcga tcttttcagc ggaaggaatc tttttgatcg attcccagca gcttcagcgc
     9841 atgggctttg aagactacgt gaagaaggga taggcagacc gcggcgtgcg ttgtgaaaac
     9901 agacagcgcg cgccgcggca gccgccggat tgggaaggca actacttctt gacgtagccg
     9961 agcacgccac ccgtcgagct ggcggtcaag gcaggcgagt ttacagccaa gctgtagtca
    10021 cacatcgccg cattgttaaa catcggatcg accgagatgt tgccggtgcc gcagcttagg
    10081 tccgatccag tgccaaggat gttgttggtg aacgttcccg agcagctcga cagcgtgttt
    10141 ggccaaatga tgctgtccga aagcacgagc ggattcgcga tcgggctcgg tgacaagatg
    10201 gtgctgttgt agaccttggc ggtgccacgc acaccagagc ccgtgcggta gatcagcgag
    10261 ttgcgcactg ttgccgatcc gttggtcatg ctgacggcct tgtcccctgc gtcgtggatc
    10321 accgtgtctt cgatcgtaaa ggtcgagttt gagtgatcga tcgcgtcgtc gccgatgtcg
    10381 acaacgtagc agcgtcgaat cgtctgccgc gatgcggcgc cgtcgacatg gatgccatcg
    10441 ccatcgtagc gaagcggcga ggtcgggcct cgaccgatcg atgtccacca cgagtcctcg
    10501 atatcgactt tgttgccgct cgacaaaaac tctgcgccga tgccgacgcg cgaaaccagc
    10561 gagcgacgga tcgagtgcgt gccggtgctc gggctctgga acaccatgcc ggttgcatcg
    10621 gtgaagatgc tgtccgaaac cgccagattg tgcgtgccag cgatgttgat gatcggcgga
    10681 cgtgggtggc tgacgacggt gccgttgcca gcgcccgtca gaatcaccca gtctaggttc
    10741 gatgaatccc caccgtacag gaagatgccg cgccaggcgt tcgggttcga tagcgacgaa
    10801 ccgctgatga cgtgggtcat cgcttgggca ccgcgttcgg aaaagaagtg aatcggctga
    10861 tcgaacgttc cggtcgcggt gatcttgccg ttcaccgtga gtagcgtgcc gtcttcaatc
    10921 ttgccggtgg tgtccaccat gatcatggtg cccggcagaa tggtcagcgt cgatccagcc
    10981 ggcactgtgg tgttgtgctg cgacagccgg atgttttcat ttggacccca ggtcaggtcc
    11041 gcgcccgaca ggttcgccgg catcacgcgc caggtcggat tcgattcgac agtcaggtgg
    11101 cgggtcgccg tgcgatcgcc caccgtcacc gtgaccaggt attcgcccgc cggaacagcc
    11161 gcaccgccat ccagcgtgag cgacacgctg ccgacgccgt gatagaagcg aatcgagtca
    11221 tcgggtaccg gaaggtgatc gtcaaatacc gtcacggtga gcggcacatc cgatccatcg
    11281 tccgctcgct tgatcgtcac cgacccgagc gtgtcgaagc agccatcggt cacgatcttg
    11341 cccgtggccg gatcgagcat ctcggcgcgc aatgtcagcg acttgtcgct caccatccgg
    11401 cgaggcatcg tcacccgcag gtccagatcg atttcgcaat gctcacccgc ggttcccgct
    11461 gggcacgcgc agctgtagct gttgacgccg tctacgcaag tgccgccgtt ttcgcaggca
    11521 tttgcaggac agtcgttgat gttttgttcg cagaactgcc cggtgaaacc agacggacac
    11581 tcgcaagacg cggtcgcccc gtcatagtga cagacaccac cgttttggca cggagccaag
    11641 ctgcacggat caccatccgg agggatgatg tccggaggca acgtcatcga ttcgttcgtg
    11701 ttgtcgctgc agcctccgcc caaggccaac gccacgagcc acgatgcaat gtgcaagcgg
    11761 ctctttccga tcaacttatt catcgagtcc tcctcaatgc gcagattgcg cactccaaca
    11821 agttatcgac attacgtcat ggcggggtcg aaggctagtc gataactagc ttgtagacat
    11881 gcttccatca ccaataaaaa acggaccaaa ccaagcaact tttttctcgg tttcacgaat
    11941 caacacaaac cgggcggctc catcaggcat atgcctgaat catcgtcaga cacagaaaca
    12001 atctgccgtc ccgctccgag cggaaagcgc cacagccgac ggcatccaac aagaacctat
    12061 tgaatctcta cggcagtttt agataattgt tcgtgggcag gcgaagtggt gtctagatgc
    12121 agcagtctgg ggtctgtaga caccaatttc agtcccgcca ttcatggcag gaaactgcct
    12181 acaacccagc atcccaagca accacgcttg cttcactggc atagacaatc tgcctccggc
    12241 agtattgata acatcacttc gtacaaggtc cattgagcat gtcactatcc agcatatttc
    12301 gtcttcgcag aaatcgaacc atcctgctcg tttcactcct tttcggccca ctgctcacgc
    12361 tatggatcgg tgcctgccaa agcgaagtca cgatgggcac agaccctgcg ccgccggaca
    12421 tggcccagcc cgttgacatg gcgcagcttg acaccgcccg gctgctgctt ctgcgcagcg
    12481 cccgttggcc tggtggcggc agggaaatga ccgtcggtgt gctaaaccag accacgggtc
    12541 tggcgtttgg gagtgatctg tcgacgcggc tgaccgcact gctgccggct ggcatcagcc
    12601 tcacgtccaa agtgcagaaa atggcactgc cgcccggcta caccgcgatc ctcttgccgc
    12661 ccacgttgtc ggcgacagag cgcgccgatc tggccagtgc gatcgtgcgc tttgcaaacc
    12721 agcgaccggc cagcgaacgc atcgcgctgt accgacatgg cgccacggtg cagctctttt
    12781 ccaactacct gatcgaacga accaagctga ccgaggcact cgatcgctac cagcgcggtg
    12841 caaacggcga tcagaacccg ctgccgctcg ttcaagcggt aggcccggca gcgagcgatg
    12901 tggaaaacat tggcggcagc gggcccgatg tcatgcgctc gctcgtcatc ctgaatccga
    12961 atccgcaagt ggtattcacc gggctgccgc gggtgttcgt cattgcggtg acgccggact
    13021 ccgccggact tgctgctgcg tctgctgcga ttgataccaa ccgacagaac gctttttaca
    13081 agtttgcggc atgcagcatc ccggacaagt tcagcgccaa gttccaagtg acagacatgc
    13141 tgggcgagct agaagcaagc tttccagcga cgctgcccga agagctgggc gcggcctgca
    13201 atgtggacaa catcgacacg gcaaagcgcg tctacacgcc aaaaatcgag ctggtattcg
    13261 acgcaaccca gcgggctgcg cacgatgcgc gaatcaaagc ggcagaagtt gcgccctacg
    13321 atgacgtgct ggcgcgcagt gactttgaaa cccaggtccg gctggccccc ggacaaccga
    13381 cggtgctggc cacagcgcat ctgcacggaa acagctcgat ccgctgccca cgccacagct
    13441 acatcatcag cctgacggga tcagatcgat accttttgcc agattccgcg agtgatgaat
    13501 atacgctgat ttcgatgtgc aatgatccag cgtatgttta tgcgccgacg gctttccggt
    13561 tgttcacgga tgacatcttc ccgctcaagt tccgattcgt cgaattcgcg attgatggaa
    13621 acacaagagg aatttacctc ttgatggaaa aaaccaaaga agagctgatc cgcgacaatg
    13681 ccagggtcac gagtgtgatg cgccgggatt atccacaagg tacaaccgac ttctttgagg
    13741 tattgtactc agatacgcct gatttaaacg atccaatcaa tcgttggaag ttcttcgctg
    13801 cccgaattgc ggcgttatcc ggcgatgact tggtttctgc gctgcgcagt caactggatt
    13861 tggatcaata tctacgttat atagcagcac aatcggtcct aaagtccgga gattacatcg
    13921 acgaagtctt cttctatgga gcagaacagg ccaatggcgc aggcggcgtc accgagacat
    13981 atcgaatgat ggcttgggac ccagagggat acagcacttg ccacgcgggc ggcgtgaacg
    14041 catatcccga tcccaatcag ctttcctatt gttcggaagg aaagctggac tggaaaatcc
    14101 tggctgacca aaaggtctac tacctgcttg tgaagaaaat cgaagatgcg ttaaacacga
    14161 cgctgacgcg cgaaaaaatg ctggccagct tgacgcagac caagaccgaa ctaaacgcac
    14221 tgttttctac gccggcggtc tgcgcagcga tgacggaatt gctgaaaatc aacgcgggtg
    14281 ccgccgactg cacagtcgcg cgcagcgtga tttcggcgcg tgccgatgca attttggcgg
    14341 cgtatgacgc gcgtcgcgcc gccctgcttc cacttatcgc cagctatcgc aacaaatatc
    14401 cgtaagcacc catcggacgc ggacatcgcg ttgcaagcgc tggagcgccc gctggatgtt
    14461 tgactaaaga cccgtgcagc agcaggccac cgcccccaca ccgagcagcg gttcgttgca
    14521 ctgaaaaccc ttcatcatgt ttccagcgca cagcgtgttg tcgaacgctg cgccaccgta
    14581 gcggtttttg tcacccggaa ccgcgcagcc atagttctgg cagcccaggt tttggcttgc
    14641 acagtattcg gcgcaactgg tgtgcttttc gagcgtcgat cccgtcatgc acttggacag
    14701 ggtgggctcg cctttttgct ggggcggacc gctttggccg atgccgagcg tggcacacga
    14761 ccatgttccc agcgcgagca gcaggccaca ggtggcgcca agcccaaacg tccaggtctt
    14821 ggcagtcaga acagagcgat cgagattttt acgcatacga catcataaac cgcccttgca
    14881 ccgtctggac agtaggcgac gcggcacttg acaccacctt ctaccaagtg gtaagtgctc
    14941 gcaccgcaac cccccggagc attcatgatc ttacaggcac tactactcgc ggtcagcgag
    15001 ggagccgagg aaaaggcccc acccgtcatc gacatcgacg gaacggtgct cgtgcagttc
    15061 gcgatcttca tcgcgatgtt catcgtcctt cgccagttcc tctttaagcc ctacatgcag
    15121 atgcgggaag accgagcgca tcgcatcagc tcggcggaaa ctgccgctgc ggagacgcag
    15181 aagcgggccg cccagctcga aaaagacttc aaagagaaga tggacaaggc caagcagtcg
    15241 gctgaagacg agcggctcaa gctcagtcat gaaggtcggc agcgggaaga agagctcttg
    15301 tcgtcggcac gcgagcgggc gcaaggtcgc attcgtgcca cccgtgagca ggtcggcgca
    15361 caagtggccc aggcgcaaac cgagctttcg cagcgagccg aagcactgtc gaaagtgctc
    15421 gcggccaagc tgctcgggcg ggaggtctaa cccaatgaag acccgtacct cgattgcccg
    15481 tctttcgctt ggacttttgg tgctgtcggc ttccacgctg gccagcgcac aagttccgga
    15541 agcgccggcg gtgaacaagc cggaaggcat cgccaagccg gcaccgattc acaacccaag
    15601 cggccaatac ccgcccctgc gccaagctgg acaggcggct gcaccacaag ccgctcagcc
    15661 ggcggcacaa cccgaagccg cagcccccgc ccagcccgcc actgcggatg ccgtgcctgc
    15721 tgcccaaccg actgaacacc cggcagcggc tccgcccgca ccgacggaac cggcccacgc
    15781 ggaaccggcg catgccgagc ctgcgcatcc agccggatcg gaacatgcgc ccccccacgg
    15841 ggaagccgcc accgcagatc atggtgccgc ccacggtgag cacgctgctg cgcacggtga
    15901 acatggcggg gcccacgaag ccggcccggt gatcgaaaac tggtggagct gggactacgg
    15961 ccccggcaag acccatcacc acccgccgtt tggcttcgcg ctcatcaact ttgcggtgtt
    16021 cttgctcatc ctgaacaagc tcgcgggcaa gagcttcaaa gagttcctcg cgaaccgcca
    16081 cctcgaagtc cgcaaggcgc tggatcgcgc gcgtgagctg gaaaagcaag cgcaggacca
    16141 gctggccgag tacgagaaga agagtcaggc gatgaccgcc gaggtggacg cgctgctcaa
    16201 gtcgatgaaa gagcaagcgc agatcgagcg gcagaagatc attgccaagg ccgaagccga
    16261 ggcggagaag ctgctcaaag acgccgaagc gcaggttcag cagaccatcg aaggtgccaa
    16321 gcgtgacctc gagcgcaaga ccgcactttt ggccgtcgat ctggcggaaa aactggtcgc
    16381 cactcggctg agtgacatgg atcagcgcaa ccttgtggag cgctttgtca cccaggttga
    16441 gacgcagtcc acccacactg cgacgggagg tcgcctatga ttagtggctc cgcctcgcgg
    16501 cgttatgccc gcgccatcct gggcatcgga caatcccagg acaaccttga gtcgctcacc
    16561 gccgaagttg agcggctggc cagcgccatc gaacacagcg ccgatctgcg cacggtgctg
    16621 ctcaatcccg cgtttacaca gacccagcgt caggatgttc ttggcaccgt ctgtcagcgg
    16681 ctggccctgt cgaagaccag ccaaaacctg gcgcttttgc ttctgtcgcg cggtcgggtc
    16741 gccaacctgc cgggcatcgc acgcagcctg cggcaaatgg tcgatgaagt ggccggtcgc
    16801 gtgcgggttc gcctgacgtc ggcgcgtcct ctcgatgcag cggcagaaac acggattcgc
    16861 tcggcgcttg gcaaggccac cggcaagacc gtgctggtcg aaaagaacgt cgacgagtcg
    16921 ctgatcggtg gcgtcgtcac ccaagtgggc gacgtggtct acgacggcag cctgcgcggc
    16981 gaactgacca gcctgcgcgc acgctggcaa gggtagttac aagtcatctt gtgccactcc
    17041 ttatccgagt ggcgttaccg atattggccc cccggtgctg cgatagcacg ggcggcagga
    17101 gcagatatgg aactcagggc cgaagagatt tcccagatca tccgcaagca gatcgaagac
    17161 tacgaccaga aggtgtcggt ccaagagacg ggcaccgtgc tgatggccgg cgacggcatc
    17221 gcgcgtgtct acggtctgtc gggcgccatg gccggcgaga cgctcgattt cccgcacggc
    17281 atcaagggca tggtcctcaa cctcgaagag gacaacgtcg gcgtcgcgct gcttggtaac
    17341 tacgagctga tccgcgaagg cgatgtcgta aagcgcaccg gacagattgc atcggttccg
    17401 gtcggcaaag gtctgctcgg tcgcgtcgtc aacgcgctag gccagccggt cgacggcaaa
    17461 ggcccgcttc agtccaccga gtcgcgcaaa gtcgaagtca aggcgcccgg catcgtctac
    17521 cgcaagtcgg tccacgagcc gatgcagacc ggcatcaagg ccatcgacgc gatgatcccc
    17581 gtcggtcgtg gccagcgtga gcttatcatc ggtgaccgtc agactggcaa aaccgccgtc
    17641 gccatcgaca cgatcatcaa ccaaaaaggg cagaacatgt tctgcatcta cgtcgccatc
    17701 ggccaaaagc agtcgacggt ggcgagcgta gtcgacaagc tgacccagac cggcgccatg
    17761 gaatacactt gcgtggtcgt cgccggcgcg tcagagcctg ccccgctgca attccttgcg
    17821 ccctataccg gcgtcaccat cggcgaatac ttccgtgaca acggca
//
LOCUS       DAORGN010000096        17757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1289_length_17757_cov_6.144842, whole genome shotgun sequence.
ACCESSION   DAORGN010000096 DAORGN010000000
VERSION     DAORGN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 17757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17757
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_17757_cov_6.144842"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKL17_11870"
     CDS             <1..372
                     /locus_tag="PKL17_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNK45476.1"
                     /translation="AFLVVKYFEYSHKFHLGTLPGAGPLGHWNPDWAALAHEHGTAFV
                     EKLKSVESPYIFIGIYFLMTGLHGLHVIIGMIAITWALVRAMRGDFHSKHFFPVECVG
                     LYWHLVDLIWIYLFPLLYLVG"
     gene            376..990
                     /locus_tag="PKL17_11875"
     CDS             376..990
                     /locus_tag="PKL17_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNK45477.1"
                     /translation="MTQAHTNHNDSHGDDHDEPHITPLSTYLGVWAALVVLTAFTVIV
                     SRFDFGSANTIVAMLVATVKATLVALFFMHLLYDDKFNLVILLTSLLFVAIFFSPVLI
                     DLGSRGMIDPLKTRAGYKILNAKPSTLPEPAPEAAPAPAAAPAATAPAAEPTAPAAAP
                     AAPAAAPAAPAPAMKPAAAPAAAPAAVPATKPAAPAPAHAAQPH"
     gene            complement(987..3635)
                     /locus_tag="PKL17_11880"
     CDS             complement(987..3635)
                     /locus_tag="PKL17_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNK45478.1"
                     /translation="MRSVILGISGPYHELAACLLVDGNVACMIEEERLSRVRHSKAAR
                     IDNAAEWPKLAVTACLRDAGLGFADIDHIAYPFIPSERLHNIGKDPDPEPDGFGSEPG
                     ERRFVADVLSIEDALRQAGGPSARFRFHYMPHHLAHAASAFLPSPFAEAAVLAVDGIG
                     EYDTTWLGQGIGDDLQMIKTLAYPHSLGFLWEMVSEALGFGPYGATKVMGLAAYGDPQ
                     RYAQRLASIIRLGDEGTFVVDHDALRFRCRGPSWVARVLPEAEVTSMQARGQASADLA
                     AALQAQTETVLIHLARFLKRTTNQDRLCLAGGVALNCAANGRLAREGIFAELFAQPAA
                     NDAGTALGAAMWLYHRMHAEKPRFTLTTARLGPVHTAEEIAAAVAASSLPWRRVPDPA
                     AEAAERLARGEICGFYRSERAEVGPRALGGRSLLADPRDRASRERINQSVKRRESFRP
                     FGPAVMAEHADDWLCIPPAARPLLPFMLAAVPVRPAQADRILAVVHADGTTRPQLVPP
                     DGSVWRALLEQFYLRTGVPMVLNTSLNVQEPIVETPEQALRLVAECGLDALLLDDVLV
                     VAKSEPSLAEAPAAALRFEPTQDRFGMRLSLVFGNEAKDGVCPFYRRDECSHCDIGAG
                     EAKAATADSHAQRWNFFAHHYRRELPQVNHLVLYNSGSLLNPKEMSPLALGALCDEAA
                     KLPSLRMLSLDTRELFVQRDRLSALRSRLPAQVSLRIILGLESADEEVRLIYLNKRMP
                     TPAIEQAVAELAASGDNVGLWLSLVFAPPGRRGEAAVTDLVEGIRYGVALAQRHRLPV
                     DFNIHPFYPSKRSLRAHPDHPRADVAQLGLALSAAQTELVDLGSDASLFVGWQDEQHD
                     QRQDLRLAELALRGNKEREAEESAAD"
     gene            complement(3778..5418)
                     /locus_tag="PKL17_11885"
     CDS             complement(3778..5418)
                     /locus_tag="PKL17_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45479.1"
                     /translation="MKRVRSFLLLSVFALSCNPASIGGETDCPDGETNCLSDDPSKHP
                     PGGPGAYDKVGPGTQTPFDPSDDGSSGVKVDKDGNIVLDPKSFGGTLSPIIWVANSAE
                     GTVSKIDTRTMVEVGRYYTHPGGNADPSRTTVGLNGDVVVANRSASDGNRASAVHIAG
                     DKSQCIDRNGNGKIDTSENGKPMAWAEGQPNNPTDECILWFTKLTANSLPRAAGFNAG
                     LGETDTVVYVGLYSTRELVRLNAKTGAIMKTISVAPAMPYGLVLDKNGDVWIRGAEGS
                     LAVVYASKGDQVKVYNGAKAPACAYGIAADSAGRIYTAGGSCVGRFDPVTETWENLDL
                     TAQGMQSGRGLAIDSKTGVWIADTYTGMYHIDGSGPKMVFKGKTVNLSSNNVGAAIDF
                     DNNPWVISQANSAAYKLNPADYSTTKVSVGTGPYTYSDMTGYQLRNAGAPSGFWRHTF
                     LGCMGAASTRWLELAYMLQAPPGTNVTIRIRSANDKTALQVAPWVEVGKVPSDTSPIK
                     IPATSGSLLQVEFGMKSSVRDVTPILSGASAGYSCVFG"
     gene            5541..6941
                     /locus_tag="PKL17_11890"
     CDS             5541..6941
                     /locus_tag="PKL17_11890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK45480.1"
                     /translation="MKHSAESSAAARDGVEKDPNSTSVLDDIPEFTRYRPGGVFLVIS
                     GPDRGEQVVLGDRPITIGSSPSCDLVLSDRTVSRSHATALLENNQIVVRDLGSTNGSF
                     TYGARFKEIVVGFGTEITLGKTVLKFLPQEEALKPQESEEDRFGSLLGCDAKMRRLFG
                     LLRDISPTDSTVLIQGETGTGKELVAEEIHRHSRRASGPFVVFDCGAVPKELIESLLF
                     GHVKGAFTGAISDRQGAFAEAHGGTIFLDEIGELALELQPSLLRALDKRAVRPVGGSA
                     YRQVDVRVIAATNRDLREEVARKAFREDLFFRLAVIRVQLPSLRERGRDIELLTESFI
                     ESFSHGKSVALPPAELARLRSYGWPGNVRELRNVIERACVLAKGDTLSLEELGDTVVH
                     SAPPAVRSSLPFKEAKGQLVEHFEREYIVDLMKRHRMNLSAAAREAQIDRKHLRELLR
                     KYGLDNRLDPADRSGE"
     gene            complement(6930..8087)
                     /locus_tag="PKL17_11895"
     CDS             complement(6930..8087)
                     /locus_tag="PKL17_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNK45481.1"
                     /translation="MSERLEWRDLSLLLVLVALGVFFGLLSPQFLSARNLSMLTIEFA
                     ITSVLSLGMLLVILPGHIDLSAGSGVGLVGGVSAVLIMRYALPAELAMLIGVGLGLLL
                     WRFQGVVIVRQGIPAFIITLGGLLACKGLFWLCIRNQTVPVSPGGQSNLLSVLTTTYL
                     PPRASYVLTACIVAVLALLTWRSHKRTTRDALAHGESPPDYELPFLRWFLSSQALGLL
                     AIVCGQFRGLPLPLLILAAVAVAVQVVTRHTRLGRALYAIGGNEEAAVLSGIPVAKVV
                     VTAYTLLGGLVALTGLLQTAYAGASTTTVGELMELDAVAACVIGGCSLRGGRGSVSGV
                     LLGSLVMASLLSGMSLLAVPPEARLIARGLTLGLAAWLDVRLAKVSRGSLA"
     gene            complement(8084..9655)
                     /locus_tag="PKL17_11900"
     CDS             complement(8084..9655)
                     /locus_tag="PKL17_11900"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNK45482.1"
                     /translation="MSAPVIELRNVSKRFGGIVALRGVDFVLGPGEVHALCGENGAGK
                     STLIKILSGVHPAGSYDGDYLLCGDKAQFASVRDSQARGISVIYQELPLCEELSVAEN
                     IFLGSEPRRTGAWPLRLLIDEDRMRRESKQLLARFGIVLEPDRKVRSLGAGQKQLVEI
                     ARALRNRSRVLILDEPTAALTDRESEALIELLRELRREGQSIVYISHRLDEVMAIADQ
                     ITVLRDGQSVFTAPASETTQTLLVQKMVGRELGELFPPRPTGVLSTPPLLSLYHLSVG
                     EQPEGPARLLDVSLQVSAGEIVGIYGLLGAGRSELLLHLFGAWGFRQSGSVRLNGKPH
                     APQNPSEAIAAGLLLVTEDRRRFGLVMDESVRFNLTLSSLASRMRHGLIDEPSELATV
                     QALSARLRIRAQNPDQAVRTLSGGNQQKVVLGKALLCQPKVVLLDEPTRGVDVGAKQE
                     IYALLDELRREGMGVLMVSSDLPELLGMSDRVLVLADGTLKQSLAASDATPEVLLHAA
                     IGPKPATNSAEADPR"
     gene            complement(9665..10756)
                     /locus_tag="PKL17_11905"
     CDS             complement(9665..10756)
                     /locus_tag="PKL17_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNK45483.1"
                     /translation="MQRASQSEPKSPSLAGRGQRLLLVTASLILSVLCGVSLSQKGRS
                     EEAPQKKVPRIGLSFDTLKEERWQRDRTAFVTRAKELGAEVIDLAANSSDVQQMRDVE
                     SLLTSQVSVLVVVAHNGAAMSKAVRLAHAAGVPVIAYDRLIPEPQLDFYISFDAVAVG
                     EKQARYLVDHLPTPGRGKIVRLHGAKIDNNAHLVKEGQDRVLSPLLQSGAIEVVHEDW
                     VDDWKPEHAKRIVNAAIAKGLQFDAILAPNDGTAGGAVQALREDGRDGKVLITGQDAE
                     LSACQRIAAGSQTMTIYKPLKLLAGRAAEIAVAMAKGRPVVARGEIAVEKAQVPAIFV
                     EVLAVTKDNLRETVVADGFHPQQAIFASP"
     assembly_gap    10891..10990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11351..11740
                     /locus_tag="PKL17_11910"
     CDS             11351..11740
                     /locus_tag="PKL17_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45484.1"
                     /translation="MSAKNPPVPAHFEDQLDALLVRIKAEGWDKRFSIDAKSMDAGLK
                     EHRAAKQKDAELRQAFEQHHKALLDDQVVLYRQYVEALGVLRAAHRSQPEVLRSLDGF
                     KRKSSGSRKKKAPLTPPQEPARPSVAQ"
     gene            complement(11791..13263)
                     /locus_tag="PKL17_11915"
     CDS             complement(11791..13263)
                     /locus_tag="PKL17_11915"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNK45485.1"
                     /translation="MSNIKSYRQDASGQKLVTVIPGDGIGPEVIHATQRVIHATGVKI
                     AWETCEAGERVFLRGLPSGVPQETIASIHRSRVVLKGPLSTPVGKGEKSANVTLRKVF
                     ETYGNIRPVRTLASVPGPYQNRNIDLVVVRENVEDLYAGIEHMQTAHVAQCLKLISRQ
                     GCEKVARLAFAVARAEGRKRVHCVTKANIMKLTEGLFQRVFDDVARDYPEIAASHILV
                     DNAAHQLVRAPEQFEVLLTTNMNGDILSDLTSGLCGGLGFAAGANLGSEVAIFEAVHG
                     TAPTIAGQDRANPCAVLLSAVMMLRHLDELAAAQQIEQAIYLALEDGVYTEDVLRDGA
                     VGTQAFADAVISRLGQAPRTTPMRTAAPLRLPIIDREKPTPRPKERQTIGVDIFVEAQ
                     TTPAELGPLMEWLCKTTPLKLKMISNRGAKVYPDRGALTECVDHWRCRFILREPSDTL
                     DDATILALLQQVSVKLRWMHVEKLELWDGQPAYTRAQGED"
     gene            13359..13694
                     /locus_tag="PKL17_11920"
     CDS             13359..13694
                     /locus_tag="PKL17_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNK45486.1"
                     /translation="MAIGRTTESLPFPEWTFLTNHAHVLIFLSQRPDALLREIALAVG
                     ITERAVLRIIGELADCGYLLRERDGRRNRYVVRADGMLRHPIERHRSVQALLDLVSGG
                     EAAQKSGSG"
     gene            complement(13704..14072)
                     /locus_tag="PKL17_11925"
     CDS             complement(13704..14072)
                     /locus_tag="PKL17_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45487.1"
                     /translation="MRVDASLWKRAAAMLGASLTMGCSSPDMKAIQVHWQLVDGRSCA
                     DAGAVRAVIQLEDGSEFTGRCSLLPDGNRIDIPSVTANSKITARAESAGLAVLYRSSL
                     TLPSMLPDVVEVVLSYSGGR"
     gene            complement(14383..15465)
                     /locus_tag="PKL17_11930"
     CDS             complement(14383..15465)
                     /locus_tag="PKL17_11930"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNK45488.1"
                     /translation="MKSIFILTSGGDAPGMNAAVRAVAKVAAAAGVRTFGVLDGYDGL
                     MDGRFRELTRAMGSGLSVIADLDTAGSLGGTMIGSARSTRFRTPEGRRQAVSKLADAA
                     GLIVIGGDGSLTGAHLLASECSTPIMGIPASIDNDIGCTATAIGVDTALNTIVDCCDR
                     ICDTAISHRRAFVIEVMGRQSGYLAMASAVAAGADAVLFREQARSEDEIIGTVAGAIR
                     NAFDKDSGKRRVLILKAEGVEIPCTRLVRRVEDVLQPVLPGVEIRATVLGHLVRGGRP
                     SFQDRMVAARLGRAAVAAVLRGESDHMVSWLQPAQGALPTEDPSVFRYPLSKVLSETK
                     SLLDGSSPVTKWRVRMMGEMSGVLGL"
     gene            15560..16894
                     /gene="trmFO"
                     /locus_tag="PKL17_11935"
     CDS             15560..16894
                     /gene="trmFO"
                     /locus_tag="PKL17_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNK45489.1"
                     /translation="MPFVPEITVIGGGLAGCEAAWQLAERGHRVDLIEMKPLRMSPAH
                     TTPLLAELVCSNSLRSDDPMTPAGLLKAELRGAHSLVIGCADATRVPAGDALAVDRVL
                     FSRLVTAKVTRHPNIRLRRQLCDELPSGPCILAGGPLVDGGAARTLRRLCGDRLYFYD
                     AIAPIVDADSIDPDRCFRGSRYHEGEEGDYLNCPMNQAEYEAFVQELLSARKVTPHRF
                     EEPRYFEGCLPIEVMAERGKDTLAFGPMKPVGLVDPRNPQTPPYAVVQLRAENRYHTA
                     YNLVGFQTRLAYPEQKRIFAMIPALAHAEFIRYGSIHRNSYVEGHKVLDESLALRAAP
                     QVHLAGQITGVEGYIESTAMGLLCALFLSDKLRGRPLPLPPSTTAIGGLYHHVVTPRL
                     AGEKYVPMNINFGLLPPFVGKEAKRAGKRGRRALHAERAREHLPAWLSQIGA"
     gene            complement(16891..>17757)
                     /locus_tag="PKL17_11940"
     CDS             complement(16891..>17757)
                     /locus_tag="PKL17_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNK45490.1"
                     /translation="LEPTAALTEQSAKLLLRAYDAFPDPVFVKDLSHRWVAANRAFFE
                     LFQLPPSAVLGKSDPDFFPKDQADVFWKHDDELFASQQPSEKEEKLTDASGTVRTIWT
                     RKYPLYGDDGALIGLCGIITDITKVRATIEQDFRLAAEVEEQQRVITAQSRLLDELAV
                     PVIRIWEGILLLPLIGEITAQRAMQFEDSLLRAISEHQAEFALIDITGVPLVDSTVAG
                     YLLRSNLSARLLGCQTIMVGIGAGVATQIVRLGADFSVILTRSTLQQGLEFAMQHARS
                     ARTLASPLRKTR"
BASE COUNT         3311 a         5621 c         5372 g         3353 t
ORIGIN      
        1 gccttcttgg tcgtgaagta cttcgagtat tcgcacaagt tccacctggg cacgctgccc
       61 ggcgcaggtc cgcttggaca ctggaacccc gactgggccg cacttgcgca tgagcacggc
      121 acggcctttg tcgaaaagct caagtccgtc gagtcgccgt acatcttcat cggaatctac
      181 ttcctgatga ccggactcca cggactccac gtcatcatcg gcatgatcgc catcacctgg
      241 gcgctggttc gcgcgatgcg tggcgacttc cactcgaagc actttttccc ggtcgagtgc
      301 gtcggtctgt actggcacct cgtcgacttg atctggatct acctgttccc gctcctctac
      361 ttggtggggt aagtcatgac gcaggcacac acaaaccaca acgattcgca cggcgatgat
      421 cacgacgagc cgcacatcac gcccctttcg acctatctgg gggtctgggc tgctctcgtg
      481 gtgctgacgg cgttcaccgt catcgtctcg cgctttgact tcggttccgc caacaccatc
      541 gtcgccatgc tggttgcgac ggtgaaagcg accctggtcg cgctgttctt tatgcacctg
      601 ctttacgacg acaagttcaa cttggtcatc ttgctgacca gcttgctgtt cgttgcgatc
      661 ttcttttcgc cggtcctcat cgacttgggc agccgcggca tgatcgaccc gctcaagacc
      721 cgcgctggct acaagatatt gaatgccaag ccgtcgacgc tgcctgagcc tgcacccgaa
      781 gcggctccag ccccagcggc ggcccccgcc gcgacggctc cggcagcgga accgacggct
      841 ccggcggcag cacctgcggc tccagcggca gcacctgcgg ctccggcccc ggcgatgaag
      901 cctgctgcgg cccccgccgc ggccccagcg gcagttcccg ccaccaagcc cgctgcaccc
      961 gcgcctgctc acgctgcaca gccgcactag tcggcggcgc tttcctccgc ttccctctct
     1021 ttgttccccc gcaacgccag ctccgctaga cgcaagtctt ggcgctggtc atgctgttcg
     1081 tcttgccagc ccacaaacag cgaagcgtca ctgcccaagt ccacaagctc ggtctgcgcc
     1141 gctgaaagcg ccagccctag ctgcgccaca tcggcgcgcg gatgatcggg gtgcgcccgc
     1201 agcgaccgct tcgatggata gaacgggtgg atgttgaagt cgacgggcag gcgatggcgc
     1261 tgcgccagcg cgacaccgta gcgaatcccc tcgacgagat ccgtgaccgc cgcctcacct
     1321 cggcgacccg gtggcgcaaa caccaaactc agccacaggc cgacgttgtc gccgctcgct
     1381 gcaagctctg ccacggcctg ttcaatcgcg ggcgtcggca tccgcttgtt caggtagatc
     1441 agccgcactt cttcatcggc agactccagc cccaagatga tccgcagtga cacctgcgca
     1501 ggcagccgcg accgcagggc cgaaagccgg tctctttgca caaacagctc tcgcgtatcg
     1561 agcgacagca tgcgcagaga cggcagcttg gccgcttcat cgcagagcgc accgagcgcc
     1621 aacggcgaca tctctttggg attgagcaga gaccccgagt tgtagagcac aaggtgattc
     1681 acctgcggca gctcgcgtcg gtagtgatgg gcaaaaaagt tccagcgctg tgcatgggag
     1741 tctgcggtgg cggcctttgc ttcgcccgca ccgatgtcgc agtggctgca ctcgtcgcga
     1801 cggtagaacg gacagacgcc gtcttttgct tcgttgccga acaccagcga aagccgcatg
     1861 ccaaaacgat cctgcgtcgg ctcaaagcgc agcgccgccg ccggggcttc ggcaagcgaa
     1921 ggctcggact ttgccaccac cagcacgtca tccaaaagca gcgcgtccag accgcactcg
     1981 gcgacaagcc gcagcgcttg ctccggtgtc tcgacgatag gctcctgcac gttcagcgac
     2041 gtgttcagca ccatcggcac gccggtgcgc agatagaact gctcaagcag cgcgcgccac
     2101 accgatccgt cgggcggcac aagctgtgga cgggttgtcc catctgcgtg aaccacggcc
     2161 aggatgcgat ccgcctgcgc tggcctgacc ggcaccgccg ccagcatgaa cggcaaaagc
     2221 ggccttgccg cgggcggtat gcaaagccag tcatcggcgt gctctgccat caccgctggg
     2281 ccaaacggac gaaaggactc gcgacgcttt accgattggt tgatgcgctc tcggcttgcg
     2341 cggtcgcgcg gatccgcaag cagcgagcga ccgccgagcg cccgcggacc cacttccgcc
     2401 cgctccgagc gataaaagcc gcaaatctcg ccccgcgcca agcgctcggc agcctctgcc
     2461 gccggatcag gcactcttcg ccacggcagc gatgacgccg ccacggcagc cgcgatttct
     2521 tctgctgtgt gcaccggccc aagccgcgcc gtcgtcaggg taaagcgcgg cttttcggca
     2581 tgcatgcgat ggtaaagcca catcgccgcc ccaagcgccg tccccgcatc attggccgcg
     2641 ggctgtgcga aaagctcggc aaagatgcct tcccgcgcca gtcgaccatt ggccgcgcag
     2701 ttgagtgcca ccccgccggc cagacacagc ctgtcttggt ttgtcgtccg cttgagaaag
     2761 cgcgcaaggt gaatcagcac cgtctcggtc tgtgcctgca gtgctgcggc caagtcggcg
     2821 ctggcttgcc cgcgtgcctg catcgacgtg acttcggctt cgggcagcac ccgcgccacc
     2881 cagcttggcc cgcgacagcg aaaacgcagc gcatcgtggt cgaccacaaa cgtcccctcg
     2941 tcccccagcc ggatgatcga cgcgagacgc tgcgcgtatc gctgcgggtc gccgtaggcc
     3001 gccagtccca tcaccttggt cgcgccgtag ggcccaaatc ccagcgcttc cgacaccatt
     3061 tcccacaaaa aacccagcga gtgcgggtag gcgagcgtct ttatcatctg caagtcgtcg
     3121 ccaatgccct gtcccagcca cgtcgtgtcg tattccccga tgccatcgac cgccaaaacc
     3181 gccgcttcgg caaacggcga cggcaaaaat gcactcgcgg cgtgtgccaa gtggtgcggc
     3241 atgtagtgga agcgaaagcg ggctgatggg ccacctgcct gacgcagcgc gtcttctatc
     3301 gaaagcacgt cggcgacaaa gcggcgttcg ccgggctcgc tgccgaaacc gtccggctca
     3361 ggatctgggt ctttgccgat gttgtgcagc cgctcgctcg gaatgaacgg ataggcgatg
     3421 tggtcgatgt cggcaaagcc aagtccagcg tcgcgcaggc aggctgttac ggccagcttg
     3481 ggccactcgg cggcgttgtc gatccgcgcc gccttgctgt gacgaactcg gctaagccgc
     3541 tcctcctcga tcatgcaggc gacgtttccg tctaccaaca gacaggcggc cagctcgtga
     3601 tagggaccag aaatgcccaa aatgacgctt cgcatgaccg gcatgctagc gcgctccaag
     3661 ttgggtccgg ggggtcagcg tggggcagaa gatgtgatgg aaagatccat caccttgcag
     3721 gctgcggcca tcacttgctt gtgtggtcgc agggaaggtc agggcacggc ggacggtcta
     3781 accgaacacg caggaataac cagcgcttgc accagacaaa atcggcgtca cgtcacgcac
     3841 cgagctcttc atcccgaact cgacttgcag cagcgaaccc gaagttgccg gaatcttgat
     3901 aggtgaagtg tccgacggga ccttgcccac ttcaacccaa ggcgcgactt gcagcgcggt
     3961 tttgtcattg gcgctgcgga tgcgaatcgt cacgtttgtg cctggcgggg cttgcagcat
     4021 ataggcaagt tccagccagc gcgtcgacgc tgcacccatg cagccaagga acgtatgccg
     4081 ccagaaaccc gacggtgcac ccgcgtttcg tagctggtag ccggtcatgt cgctgtacgt
     4141 gtatggtccc gtgccgacgc tgactttggt ggtcgagtag tccgccgggt tcagcttgta
     4201 cgccgcgctg ttggcttggc taatgaccca ggggttgttg tcgaagtcga ttgcggcacc
     4261 gacgttgttg ctcgacaggt ttaccgtctt gcccttaaag accatcttgg ggcccgatcc
     4321 gtcgatgtgg tacatgccgg tgtacgtgtc ggcgatccac acgccggtct tgctgtcgat
     4381 cgcgaggccg cggcccgact gcatgccctg cgccgtcaga tccaggtttt cccacgtctc
     4441 ggtcactgga tcgaagcgcc cgacgcagct accgcctgcg gtgtagatgc ggcctgctga
     4501 atcggcagcg attccgtaag cacaggcggg cgctttggcg ccgttataga ccttcacttg
     4561 gtcgcctttg ctggcgtaga cgacggccaa gctgccttct gcgccgcgaa tccagacatc
     4621 gccgtttttg tcgagcacca gtccatacgg catcgcgggc gctacgctga tggtcttcat
     4681 gatcgcgccg gttttggcgt tcagccgcac cagctcgcgg gtggaataca ggccgacgta
     4741 gaccaccgtg tccgtttcac caagcccggc gttgaagccg gcggcgcgtg gcagcgagtt
     4801 ggcggtgagc ttggtaaacc acaggatgca ctcgtcggtc gggttgtttg gctgcccttc
     4861 agcccaagcc attggcttgc cgttttccga cgtgtcgatc ttgccgttgc cgttgcggtc
     4921 gatgcactgc gacttgtcgc cagcgatgtg gacggcggac gcgcggttgc catcgcttgc
     4981 gctgcggttg gcgaccacca cgtcaccgtt tagtccgact gtggtgcgcg acgggtcggc
     5041 gttgccgccg ggatgggtgt agtagcgccc gacttcgacc atcgtgcggg tgtcgatttt
     5101 ggacaccgtg ccttcggcgc tgttggcgac ccagatgatg ggggacagcg tgccgccgaa
     5161 gctctttggg tccaggacga tgttgccgtc tttgtcgacc ttgacccccg acgatccgtc
     5221 gtcgcttgga tcaaacggcg tctgggtgcc tggcccaact ttgtcatacg cgcctggacc
     5281 gccgggcggg tgcttgctcg gatcgtcgct aaggcagttg gtctcgccat ctgggcagtc
     5341 ggtttcgcca ccgatgctcg cggggttaca ggacagtgcg aagaccgaca gcagcaaaaa
     5401 agagcgaacg cgcttcatca aatgcctcca cattcgggtc gttgcatacg ttgtgccgaa
     5461 agcgaagcaa aatccgcgcc ttacattccc ggtttgggcg cttgtgtgcc ggcactcgtg
     5521 agctaagatt ccccaaaacc atgaagcact cggcggaaag cagtgccgct gccagggacg
     5581 gggtggaaaa agaccccaac tcgaccagcg tcctcgacga cattccggag ttcacacgct
     5641 atcgcccagg tggcgtcttc ttggtgataa gcggccccga ccgcggcgag caagtcgttt
     5701 tgggcgaccg acccatcacg atcggctcgt ctcccagctg tgacttggtg ctgtctgacc
     5761 gcaccgtctc gcgaagccac gccaccgcgc tattggaaaa caaccagatc gtggtgcgtg
     5821 atctgggctc gaccaacggc agcttcacct acggcgctcg cttcaaagag atcgtcgtgg
     5881 gctttggcac cgagatcacc cttggcaaga cggtgctgaa gtttttgcca caggaagaag
     5941 cgctcaagcc ccaagagagc gaagaagacc gcttcggcag cttgcttggc tgcgacgcca
     6001 agatgcgcag actgtttggg cttttgcgtg acatctcgcc gacggattcg accgtgctta
     6061 tccagggcga aaccggcacc ggcaaagagc ttgttgccga ggagattcac cgtcactcgc
     6121 gccgcgcatc cggtccgttt gtcgtcttcg actgcggtgc cgtgcccaaa gaactgatcg
     6181 agtcgctctt gtttggccac gtcaaaggcg cgttcacggg cgccatctcc gatcgccaag
     6241 gcgcgtttgc cgaagcccac ggcggcacca tctttctcga tgaaatcggc gagctggcgc
     6301 ttgagcttca gccgtctctg cttcgcgcgc tcgacaagcg ggccgtgcgt ccggtcggcg
     6361 gcagtgccta tcgccaggtc gatgtccgcg tcatcgctgc aaccaaccgc gatctgcgcg
     6421 aagaagtcgc ccgcaaggct ttccgcgaag acctgttttt ccggctcgcc gtgatccgcg
     6481 tccagcttcc gtccctgcgt gagcgaggcc gcgacatcga gcttttgacc gagtcgttca
     6541 tcgagtcgtt ttcccacggc aagagcgtgg ccctgccgcc ggcagagctg gcccgactgc
     6601 gcagctacgg ctggcccggc aacgtccgcg agctacgcaa cgtcatcgag cgagcctgcg
     6661 tgctggccaa gggcgatacg ctcagtcttg aagagctggg tgacaccgtg gtccactcgg
     6721 cgccgcccgc ggtcaggtct tcgctgccgt tcaaggaagc caagggccag ctcgtcgagc
     6781 acttcgagcg agaatacatc gtcgacctga tgaagcggca ccgcatgaac ttgtcggcgg
     6841 cagcccgtga agcccagatc gaccgcaagc acctgcgtga gctgcttcgc aagtacgggc
     6901 tcgacaaccg gcttgatccc gccgaccgat caggcgagtg acccgcgcga gactttggcc
     6961 agccgaacat cgagccaggc cgcaagtccc agcgtcagtc cgcgcgcaat cagcctggct
     7021 tccggtggca ccgccagaag cgacattcca gacaacagac tcgccatcac cagcgatccc
     7081 aaaagcaccc cagacacgct gccgcgaccg ccacgcaggc tacagccgcc gatgacgcac
     7141 gctgcgactg catctagctc catcagctcg ccgacagtcg tggtcgatgc acctgcatac
     7201 gccgtctgca aaagcccagt cagcgcgacc agcccgccga gcagcgtata cgccgtcacc
     7261 accactttgg cgaccgggat acccgataga accgccgctt cttcgttgcc gccgatcgcg
     7321 tacagcgccc ggcccagccg cgtgtgccgc gtcaccacct gcacagccac cgccacggct
     7381 gccagaatca aaagcggcag cggcagaccg cgaaactgac cacagacaat cgccaaaaga
     7441 cccagcgctt gtgacgacaa aaaccagcgc agaaacggca gctcatagtc gggtggcgat
     7501 tcaccgtggg ccagcgcgtc cctggttgtt cgcttgtggc tccgccacgt caaaagcgcc
     7561 agcaccgcga caatgcaagc ggtcagcaca tacgacgctc gcggcggcag atacgttgtg
     7621 gttagcaccg acaacaggtt cgactgcccg ccggggctca cggggacggt ctggttgcgg
     7681 atgcacagcc agaacagccc cttgcaggcc aagagcccgc cgagcgtgat gataaacgcc
     7741 gggatgcctt gccgcacgat gacaacgccc tgaaagcgcc acaaaagcag acccaagccg
     7801 acgccgataa gcatggccag ctctgccggc agggcgtagc gcatgatcag cactgccgag
     7861 acgccaccga caagcccgac gccgctgcct gctgacaggt cgatgtgtcc cggcaaaatc
     7921 acaagcagca tccccaggct tagcaccgac gtgatggcaa actcgatcgt cagcatgctc
     7981 aggttgcgcg ccgacagaaa ctgcggcgac aaaagcccga aaaacacccc cagcgcaacc
     8041 agcaccagca gaagcgacag gtctcgccac tccaatcgct cgctcatcgc gggtctgcct
     8101 ccgcgctgtt tgtcgccggc ttgggcccga tggcggcgtg cagcagcact tctggtgtcg
     8161 catcacttgc cgcaaggctc tgcttcagcg ttccatcggc cagtacgagc actcgatcgc
     8221 tcattcccaa aagctctggc agatccgaag acaccatcag gacgcccatt ccttcgcgcc
     8281 gcagctcgtc gagcagcgca tagatttctt gtttcgcccc cacgtcgaca ccgcgcgtcg
     8341 gttcatccaa aagcacgacc ttgggctgac acagaagcgc cttgcccagc accactttct
     8401 gctggttgcc gccggacagg gttcttaccg cctggtccgg gttctgcgcc cgaatgcgca
     8461 gccgggcaga cagcgcttgc actgtggcaa gctcgcttgg ctcgtcgata agtccatgcc
     8521 gcatgcgcga tgccagggaa gaaagggtca ggttgaaccg gaccgactcg tccattacga
     8581 ggccaaagcg gcgcctgtct tcggtgacaa gcagcaagcc ggcggcaatc gcttcactcg
     8641 ggttctgcgg cgcatgcggc tttccgttga gccgcacgct gccactttgc cgaaagcccc
     8701 aggcgccaaa caggtgcaga agcagctcgc tgcgacccgc gccgagcagt ccgtagatcc
     8761 cgacgatttc cccggcagag acctgcagcg acacgtccag aagccgcgcc ggcccttcag
     8821 gctgctcgcc cacgctcagg tggtaaagcg acaaaagcgg cggcgtcgac agcactcccg
     8881 taggacgtgg tgggaacagc tcacccagct cgcggccaac catcttttgc accagcagcg
     8941 tctgggttgt ttcggatgcc ggcgcagtaa agacgctctg tccatcgcgc agcaccgtga
     9001 tctggtcggc aatcgccatc acttcgtcga gccgatgcga gatgtagacg atgctctgtc
     9061 cctcgcgcct taactcgcgc agaagctcga tgagcgcctc gctttcccga tcggtgagcg
     9121 ctgccgtcgg ttcatcgagg atgagcaccc gtgagcgatt gcgcagcgcc ctggcgattt
     9181 cgacgagctg cttttggcct gcccccaagc tgcgcacttt ccggtcgggc tcgagcacga
     9241 tgccaaagcg ggcaagcagc tgcttggact cgcgccgcat ccgatcttcg tcgataagca
     9301 gccgcagtgg ccaagctccg gtacgccgcg gctcactgcc caaaaagatg ttttccgcga
     9361 ccgatagctc ttcgcacagc ggcagctctt gatagatcac tgaaatgccg cgtgcctggc
     9421 tgtcgcggac cgatgcaaac tgcgccttgt ccccgcaaag caggtagtca ccgtcgtagc
     9481 tgccggctgg atggacgcca gacaggatct tgatcagcgt cgatttgccg gccccatttt
     9541 cgccgcacaa ggcgtggact tcgcctgggc caaggacaaa gtcaacgcca cgcagcgcga
     9601 cgatgccacc gaatcgcttg ctgacgttac gcagctcgat cactggcgcg gacatcatga
     9661 ccttttacgg gctggcaaag atcgcctgct gcgggtgaaa gccatcggcc accaccgtct
     9721 cgcgcaggtt gtctttggtc acggccagca cctcgacgaa aatcgctgga acttgcgctt
     9781 tttcgacggc gatttctcca cgcgcgacca ctggcctgcc tttggccatc gccaccgcaa
     9841 tttcggcagc ccgtcccgcc agcagcttga gcggcttgta aatcgtcatc gtctggctgc
     9901 ccgcggcgat gcgctgacag gccgaaagct cggcatcttg gccagtgatg agaaccttgc
     9961 cgtcgcggcc atcttcgcgc agcgcttgta ctgcgccccc tgcggttccg tcgttcggcg
    10021 ccaagatcgc atcaaactgg agacccttgg cgatggccgc gttcacgatc cgtttggcgt
    10081 gttctggctt ccagtcgtcg acccagtctt catggaccac ttcgatcgcg ccactttgca
    10141 aaagcggtga aagaacgcgg tcttgccctt ccttcaccaa gtgggcgttg ttatcaatct
    10201 tggcaccatg cagccgaaca atttttcctc gtcccggcgt cggcaagtgg tccacaagat
    10261 agcgggcctg tttttcgccc accgccaccg catcaaacga aatgtaaaag tcgagctgcg
    10321 gttccggaat cagtcggtcg taggcgatga ccggcactcc tgcggcatgt gcaagtcgta
    10381 ccgctttgct catcgccgcg ccgttgtggg caacgacaac cagcacgcta acctgcgacg
    10441 tcaagagcga ctccacatcg cgcatctgct ggacgtcaga cgagttggcc gccaggtcaa
    10501 tcacttcggc gcccagctct ttggcgcgcg tgacaaatgc agtgcggtcg cgttgccagc
    10561 gctcttcttt cagcgtgtcg aacgacagcc cgatgcgcgg cactttcttc tgtggcgctt
    10621 cttcgctgcg tcctttttga ctcagcgaaa cgccacacag cacgctcaag atgagcgatg
    10681 ccgtgaccaa aagcagtcgc tgaccacggc cagccaaaga aggggatttg ggctcacttt
    10741 gagaagctcg ctgcaccacg ggcgcactgt aggcagcgcg cacgctggga agcaagcctt
    10801 gcaaacgaac ccggcagaag tcggtccgct cgcatggatc aacgaatccg acatgcaaag
    10861 catgaacagg gacatgcaaa gcatgagcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn agcatgagca gagacatgca aagcatgcgc gaggacacac gtcgcatgta
    11041 gcggcgcgtg ccgtgcatga cggcctgcat gttccgcatg acgcgggtga tgtttcgcaa
    11101 ggcacggggc atgcgccgca tgtcgcgtgt cacatggggc agatccgcga tcacaagcga
    11161 cgtttgttcg gttcgcttct gtttctgttc tgcgattttt tccgacggga acgaaaaaaa
    11221 gcagaggaat tacgcaacct tggctgcggt gtaccgaaaa cgactccacc tgttgccgct
    11281 gcggctacgt ccactgactt gggcgcagct ttgcacacca accaagcgac aggaaaaaag
    11341 gagaaacggt atgtctgcta agaacccccc tgttcccgcc cacttcgaag accagcttga
    11401 tgccttgctc gtccggatca aggccgaagg ctgggacaag cgctttagca tcgacgccaa
    11461 atcgatggac gctgggctca aagaacaccg agccgccaaa cagaaagacg ccgagctgcg
    11521 ccaagcgttt gaacagcacc acaaagcgct cttggacgac caagtggtgc tctacaggca
    11581 gtacgtcgaa gcgctcggcg tgctgcgcgc tgcccaccgc agccagcccg aagtgctgcg
    11641 gtcgcttgat ggctttaagc gcaagagcag tggcagccgg aagaagaaag cgccacttac
    11701 gcccccgcaa gagccggcgc gtccgtctgt ggcccagtga cgtaggccgg cgcgttttgt
    11761 gacggtcggc tcgcgtggcc gtttgccccg ttagtcttcg ccttgggcgc gggtgtacgc
    11821 gggctggccg tcccatagct ccaatttttc gacgtgcatc cagcgcagct tgacagacac
    11881 ctgctgaagc agcgccaaga tcgttgcatc atcgagggta tcgctcggct cgcgcaggat
    11941 gaaacggcac cgccagtggt cgacgcattc cgtgagcgca ccgcggtcgg gatacacttt
    12001 ggcgccgcga ttcgagatca tcttgagctt gagcggcgtg gtcttgcaca gccactccat
    12061 aagcgggcca agctctgccg gggtggtctg tgcttcgacg aagatgtcga caccgatggt
    12121 ctgtctttct ttgggccgtg gcgttggttt ttctcgatcg atgatcggca agcgcagcgg
    12181 ggcagcggtg cgcatcggcg tggtgcgtgg tgcctggcca agacggctta tcacggcgtc
    12241 ggcaaacgct tgggttccga ctgcgccatc acgcagcacg tcttcggtgt agacgccgtc
    12301 ttccagcgcc aaataaatcg cctgttcgat ctgctgcgcc gccgccagtt cgtctaggtg
    12361 ccgcagcatc atcaccgcag acagcagcac ggcgcaggga ttggcgcgat cctgaccggc
    12421 gatggtcggt gcagtgccat ggaccgcttc aaagatggcc acttccgaac ccaggttggc
    12481 cccggcagca aagcccagac cgccgcacag acctgacgtc agatccgaca gaatgtcgcc
    12541 gttcatgttg gtggtcaaaa gcacctcgaa ctgctcgggc gcgcggacca gttggtgagc
    12601 ggcgttgtcg accaggatgt gcgacgcggc gatttcgggg tagtcacgcg ccacgtcatc
    12661 gaagacgcgc tgaaacagtc cctcggtcag cttcatgatg ttcgctttgg tcacgcagtg
    12721 gacccgcttg cgtccttccg cgcgcgcgac cgcaaacgcc aggcgtgcga ctttttcgca
    12781 gccctgtcgc gagatcagct tgagacactg cgcgacatgt gccgtctgca tgtgctcgat
    12841 gccggcgtac aagtcttcga cgttttcgcg caccacgacc agatcgatgt tgcggttttg
    12901 atacggtccg ggcaccgaag ccagcgtgcg cacgggcctt atgttcccgt aggtttcaaa
    12961 gactttgcgc agcgtgacgt tggcgctctt ttcgcctttg ccaacgggtg tcgacagcgg
    13021 gcctttgagc accacacggc tgcgatgaat cgacgcaatc gtctcttggg gcacgcctga
    13081 aggcaggccg cgcaaaaaga cccgctcgcc ggcttcgcac gtctcccaag cgatcttgac
    13141 gcctgtggca tggatgacgc gctgggtggc gtggatgact tcagggccga tgccatcgcc
    13201 ggggatgact gtgaccagct tctgaccgct cgcatcctgt ctgtacgatt tgatgttcga
    13261 catgaggctc ctcacaagac ctacatacat gaactatgat gcatgaaata tatttcatgt
    13321 ttcaagattc gtgcatcagg actagcgtgg gcacagtcat ggcgattggc agaacgacag
    13381 agtcgcttcc gtttcccgag tggacgtttt tgacaaacca cgcccacgtt ctgatttttc
    13441 tgtcgcagcg tcctgacgcg ctgctgcgtg aaatcgccct ggctgtcggc atcacagagc
    13501 gcgcggtgct gcgcatcatt ggcgagcttg ccgactgcgg ctatctgctg cgtgagcgag
    13561 acggacggcg caatcgctac gtcgtgcgcg ctgacggcat gctccggcat ccgatagaac
    13621 gtcaccgcag cgtgcaggcg ctcctcgatc tggtgagcgg gggagaagcg gcccaaaaat
    13681 caggcagcgg gtgagtgtgc gcgctagcga ccgccgctat aggacagcac gacttcgacg
    13741 acatccggca gcatcgaagg aagtgtcagg ctggacctgt acagcaccgc caggccggcg
    13801 gattccgcac gggccgttat cttgctgttt gccgtcacgc tggggatgtc gatgcgattt
    13861 ccgtcgggca gcaagctaca tcggcctgtg aactcgctgc cgtcttctag ctgaatgacc
    13921 gcgcgcacgg caccggcatc ggcgcaggat cgaccgtcca ccaactgcca gtggacttgg
    13981 atggctttca tatcgggcga ggagcagccc atcgtcagac ttgcgccgag cattgcggct
    14041 gcccgtttcc acaaagaggc gtcgactcgc atgcgcgcag cctaccagaa gcggcagtcg
    14101 tggtagcgtc atcgaaacga agcgatcgcg acaaaaccag cagaggaaaa cgatgaagat
    14161 cctgatcaag tactgcgaga tgtgaaacta caaacccaag gctgcgagtc tcgcggccca
    14221 catcaaaaag acgctgggcc acgcggtcca gatcgaaccc ggtggcaaag gccagttcga
    14281 tgtgttcgtc gatgaaacgg tcatcttttc aaagcacaag gaaggccgct tccctgagca
    14341 cgacgaggtg acttcggcga taccgaaggg ctaagcgttc acttacaagc caagcacgcc
    14401 ggacatctct cccatcatcc tcacacgcca cttggtcacc gggctcgaac catcgagaag
    14461 cgacttggtt tcgctgagca ctttggagag cggatagcgg aacaccgaag ggtcttccgt
    14521 gggcagcgca ccttgcgcag gttgcagcca cgacaccatg tggtcgcttt cgccgcgcag
    14581 gacggctgcc actgcggcgc gacccagtct ggcggcgacc atgcgatctt ggaacgacgg
    14641 acgaccaccg cgcaccaagt gcccaagtac cgtggcgcgg atttcaactc ctggcagcac
    14701 tggctggagc acgtcttcga cccggcggac aagtcgcgtg cagggaatct cgacgccttc
    14761 ggctttgagg atgagtacgc gccgcttgcc gctgtctttg tcaaatgcgt tgcgaattgc
    14821 acccgcgacg gtgccaatga tttcgtcttc gctgcgggct tgctcgcgga acagcacggc
    14881 gtcggccccg gcggccaccg cgctggccat cgccagataa cccgactggc gacccatgac
    14941 ttcgatgaca aaggcgcggc ggtgcgagat cgcggtgtcg cagatgcggt cgcagcagtc
    15001 gacaatggtg ttgagcgccg tatcgacgcc gatggcggtg gcggtgcagc cgatgtcgtt
    15061 gtcgatcgac gcggggatgc ccatgatggg ggtgctgcat tcgctggcca gcaagtgcgc
    15121 gccggtcagt gatccgtccc caccgatgac gattaggccg gcggcatcgg ccagcttgct
    15181 gacggcttgg cggcggcctt cgggggtccg aaagcgtgtc gagcgagccg agccgatcat
    15241 ggtcccgccg agcgatccgg cggtgtcgag gtcggcgatg acggaaagtc cactgcccat
    15301 ggcgcgggtc agctcacgga accttccgtc catcaggccg tcgtaaccgt cgagtacgcc
    15361 aaacgtgcgg acgccagccg ctgctgccac tttggcgacg gctcgcactg cggcgttcat
    15421 gccgggcgcg tcgcctcctg atgtcaggat gaagatgctc ttcataattc cccctggctt
    15481 ttagggtatc gcgaaacccc aagtcttggg actgactacg tatttttgca ccattgtctc
    15541 tatactgcgc gccgtgcaga taccgtttgt ccccgaaatt accgtgatcg gcggcggctt
    15601 ggccggctgc gaggcggcgt ggcagcttgc cgagcgtggc caccgcgtcg acttgatcga
    15661 gatgaagcct ttgcggatgt cgccggcgca cacgacgcca ctgcttgccg agttggtgtg
    15721 ttccaactcg ctgcgcagcg atgatccgat gacgccggca gggcttttga aggcggagtt
    15781 acgcggcgcg cactcgcttg tcattggctg cgccgatgca acgcgggttc cggcgggcga
    15841 tgcgctggcg gtcgatcggg tgctgttttc gcggcttgtg acggcaaagg tgacgcgcca
    15901 tccgaacatc cggcttcggc ggcagctatg tgacgagctt ccgagcggac cttgcatcct
    15961 ggcgggtgga ccgctggtcg atggcggagc ggcacgcacg ctgcggcggc tgtgcgggga
    16021 ccggctgtat ttctacgatg cgatcgcgcc gatcgtcgat gcggactcga tcgatcctga
    16081 tcgctgtttt cgtggctcgc gctaccacga aggggaagag ggcgactatc taaactgccc
    16141 gatgaatcag gctgagtacg aagcgtttgt gcaggagctt ttgtctgccc gcaaagtgac
    16201 gccgcatcgc ttcgaggagc cacgctattt cgagggctgc ttgccaatcg aggtcatggc
    16261 cgagcggggc aaagacaccc tggcgtttgg tccgatgaag ccggtcggtc ttgtcgatcc
    16321 gcgcaatccg cagacgccgc cgtatgcagt ggttcagcta cgcgccgaaa atcgctatca
    16381 cacggcctat aacttggtcg gttttcagac gcggctggcc tatccggagc agaagcgaat
    16441 ctttgcgatg attccggcgc tcgcgcatgc ggagttcatt cgctatggct cgatacatcg
    16501 caacagctat gtcgaaggcc acaaagtgct ggatgaatcg ctggcgcttc gggctgcgcc
    16561 gcaggtccat ctggccggac agattaccgg agtcgagggc tacatcgagt cgactgcgat
    16621 ggggcttctg tgcgcgctgt ttctgtctga caagctgcgg gggcgaccgc tgccactgcc
    16681 accatcgacc acggcgatcg gcgggcttta ccaccacgtc gtgacgccgc gactggcggg
    16741 ggaaaagtac gtcccgatga acatcaactt tgggctgctg ccgccgtttg ttggcaaaga
    16801 agcgaagcgg gcgggcaagc gtggtcgtcg ggcactgcat gccgagcggg cgcgtgagca
    16861 cttgccggcg tggctttcgc agatcggtgc ttagcgggtt ttgcggagcg gggatgcgag
    16921 cgtgcgtgcg ctgcgcgcgt gctgcatggc aaactccaga ccctgctgga gcgtcgagcg
    16981 agtcaagatg acggaaaagt ctgcgcccaa ccggacgatc tgcgtcgcga cgccagcgcc
    17041 aatgccgacc atgatggtct ggcatcccaa aagccgggcc gacaggttgc tgcgcaaaag
    17101 gtagccagcc accgtgctat cgaccagcgg tacgcccgtg atgtcgatga gtgcaaactc
    17161 cgcttggtgt tccgagattg cgcgcagcag gctgtcttcg aactgcatgg cgcgctgggc
    17221 ggtgatttca ccgatgagcg gcagaagcag aatgccttcc cagatgcgaa tgacgggtac
    17281 agccagttca tcgagtaggc gactttgggc tgtgatgacc cgctgctgtt cttctacttc
    17341 ggcagcgagc cggaagtctt gctctatcgt cgcgcggact ttggtgatgt cggtgatgat
    17401 tccgcacagg ccgatcaggg ctccatcatc accgtaaagc ggatacttgc gcgtccagat
    17461 ggtgcggacc gtgccgcttg cgtccgtcag cttttcttct ttttccgatg gctgctggct
    17521 ggcaaacaat tcgtcgtcgt gcttccagaa cacgtcggcc tggtctttgg ggaaaaagtc
    17581 cgggtcactt ttgccaagga cggcgcttgg cggtagctga aacagctcaa agaacgcgcg
    17641 gttggccgca acccagcgat gtgacaggtc tttgacaaag accggatctg ggaaagcgtc
    17701 gtaggcgcgc agcagcagct tggcgctttg ctcggtaagg gcggctgtcg gttctag
//
LOCUS       DAORGN010000097        17035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1364_length_17035_cov_5.663133, whole genome shotgun sequence.
ACCESSION   DAORGN010000097 DAORGN010000000
VERSION     DAORGN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17035
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1364_length_17035_cov_5.663133"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PKL17_11945"
     CDS             <1..927
                     /locus_tag="PKL17_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45491.1"
                     /translation="SDGGLPKVRLCDQTPPMGSTLAPPPPVYAGSCPHLVSGKNIIVS
                     SGKARTFLLAVPTTIRPAEKLPLVFMWHWLGGSADSFFKKGEVQAAVDSQRFMAILPE
                     AKGDITWKWPFDVFVTAARQEEEYRFFDDMLACAAAQFAVNTNCVSSVGVSAGALFTD
                     QLATGRNKYLASAISLSGGVEGVAKKWGNPPRKLPMLVLWGGASDICAGLVNFQTASK
                     ALETQLVKDGHFFVECVHNCGHAEPPLEASMMLTKYAAFWEFVFAHPYWLGTGESPYK
                     TSGLPSSFPSWCSVGAGTAKIRTGICTEKPGC"
     gene            complement(936..1808)
                     /locus_tag="PKL17_11950"
     CDS             complement(936..1808)
                     /locus_tag="PKL17_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45492.1"
                     /translation="MLHVPAVPIVGLIESLQSLRSQTKPQIDVVSIDAELGAIALGRP
                     HAASAKLCSLDKEEALTAVAVAKAAMQTAVALSTRMVRLRLGEIAGIDRLWRKVRAHF
                     LRGALADDETPNEELLRARSALLPRHLDAVRRSLEQLANVAVQHGLTLLLGFPRRATD
                     LPHPIELRLLLEEFDGAPLKPSLDLPAAHLASTMRLVSLSESVRAFAPGPLHLVGDAC
                     GPIGALPPGSGEVDVAAVCRALPKDSTLTFLPWIGLTAKEAMAGLHAVSRLHPAAQHA
                     EKSWPSSPVPRPAE"
     gene            2036..4090
                     /locus_tag="PKL17_11955"
     CDS             2036..4090
                     /locus_tag="PKL17_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK45493.1"
                     /translation="MQSTFGAHTHKRLGTLLGSLGALLLSSQTASAQQVQEAGFTQPT
                     YNMQLFRPAIDSKGYVTLNASQVLGHKDFSLGLVGTWAGKPLYLEVPAGSSAPFGPVS
                     SGRTFEVSNLVTAQLQFALGLFKHFEIALGIPLSIMTGGRQVCPQGQDCQGYAGSGSA
                     RTDLTFSKVGIADLAIHFKGRFLNTSRHPVGLGALLSVYLPVDKWGGGDAYKQFIAED
                     NVTIHPQILLDKEWGRSRRFRTAINAGALVRFGSTTFTDRGRPKVTDEMMNTFCYPAD
                     NTSTQFDSGLCGTGLSRSVGTQLTYGIGASLAIVKQRFEVLGEFYGYADVTGNAKAYP
                     LEGLGAIKLYLAKKSFLVAGAGGGLIGLSDSGKMTGGPLWRAFIGFIFEPNIGDRDGD
                     GIKDDVDKCPDDPEDFDDFEDEDGCPDPDNDRDGILDVDDKCPNEPETKNGFEDEDGC
                     PDSVVLDRDGDGIPDNLDKCPDDPEDKDGFEDEDGCPDPDNDKDGILDKDDLCPNDPE
                     DKDGFEDLDGCPDPDNDKDGILDKDDQCPNEPETFNGYQDEDGCPDKGRVIVRKGKLE
                     ILDKIYFETDKDIIKPISYPILDAIAATLKGNPQILEIEVQGHADERGDDAHNLDLTD
                     RRAKAVRVYLIDKGIDGNRLQAHGYGETKPVCMEHNEDCWSRNRRVEFVILKRSDGDT
                     KD"
     gene            complement(4159..5280)
                     /locus_tag="PKL17_11960"
     CDS             complement(4159..5280)
                     /locus_tag="PKL17_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45494.1"
                     /translation="MWLGLRVWLWSAGAPLGESVTVRVDSQHVAKGLLFRSAEGTKPR
                     PLLIAAHGGLATKETLLGLCYEAVSRGADCVTIDELGHGASSPVPILNTISAMRAALH
                     VERALGQSAEHTYFVGHSMGAYLGCGSAFSCSHCVAIGQSVPCEDRRMIYGRLHQQLG
                     LSAAFYLPVSHVLEPWTPSVIETAIDRALPLAKPKGATLRISMRIALAWGSLVVMMCI
                     GVILARCVRQITSIPGPLRGLLAAAIVWCALAVGSYRTLWWLLPFQRTDLLIVLSLIL
                     SALMVTMVARWLSLRHPLWGVFIAALLAEIAAVCCYAAFPAEPLRGLLRLPLGLLLPL
                     IIAVTAMEKLSRHTDDSRESALFAASLLGTFLALLVPGL"
     gene            complement(5402..6661)
                     /locus_tag="PKL17_11965"
     CDS             complement(5402..6661)
                     /locus_tag="PKL17_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNK45495.1"
                     /translation="MSTHPSVSDLEALVAGTLDEDRSIVISVHSDECPQCSREVAWLR
                     AEKELFAQRARGVPPSEVWAQVEAKIAARIGQKEQAPRGLSRILGRNFANQRAQWYAV
                     GAAAIAIFGVVAASPLSPLRKANHGNVTVTEKLNIPNPLSAMGDLSELHDGAGEEDND
                     ADAAEPEDTLTTQVKVSAPISLEIVTDAADVEVSQGAKDQAKLSLDESGVRAVKLIPP
                     ASGQGSWRLDFDGSTTLPNGALRLQLPEGSKVDVKTASGDVRVHDLKSDVSVVTASGD
                     IAVKQARSLYVQSVSGDIELHDISGTVDAKTTSGELHMGGEIVSPVRFHSISGDMTLA
                     GPCRIASCKVTAQTTSGNVTIQNDRDSSLSAKLKTKSGELLGIRELTMERKGPPRSGT
                     EWIAKLGQGVGSIELDSMSGDLHIGTR"
     gene            complement(6658..7293)
                     /locus_tag="PKL17_11970"
     CDS             complement(6658..7293)
                     /locus_tag="PKL17_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45496.1"
                     /translation="MTGKRALTLLHTSHPLAGPVEEERAVTSIFDPLVVRAQKGDSAA
                     FRALFQHHSPGVRRYLGDLLRDDAAADDATQETFLRAFDSLAQLRESARLSSWLFGIA
                     RNVFLEHCRTQKQSARRLDEPNEECEPVDDAPTPDAVLLLAEADRVLSEALTHVGEPR
                     KSALLLRLDHGLSYEDIADVMGWSVAKVKNEIHRARLQLRTQLWRYLGGTK"
     gene            complement(7392..8264)
                     /locus_tag="PKL17_11975"
     CDS             complement(7392..8264)
                     /locus_tag="PKL17_11975"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNK45497.1"
                     /translation="MNFRDSMIPAGFSEETRRIVPIILEAGALAHRYFVAWHLPGSSP
                     SSLPVEWKDAEEPVTEADRAVSELCIARIAAAYPSDVVLSEERPDDGARLSAPRFWLV
                     DPIDGTKDFIAGRAGYSVMIGLVASGIPILGAVYQPAAARLWLAERGRGAFEIIEDEP
                     PQRIQVSPISEIAEARLVSSASSRPDLMNHVRDRAGIRDEVQVGSVGVKLCLIAAAAR
                     DLYMNPAGRCKLWDTAAPEVILHEAGGKLTDMRGKPLRYSDLDLGHHHGLVGSNGQLH
                     DAALHVLEPFLSSP"
     gene            complement(8290..9729)
                     /locus_tag="PKL17_11980"
     CDS             complement(8290..9729)
                     /locus_tag="PKL17_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK45498.1"
                     /translation="MKTLLIVDDETSNLDSLERTFARFVPPHTDSESSGSELQVITCS
                     DGKDALEILRKQRIDVVLTDLMMPGISGLDLLKAGRKLSPETEVILMTAYGTIETAVE
                     AMKDGAYDFITKPLKRAHVLRVVGKALERQTLVLENRSLRQKLAAAERRTIVGGAMAM
                     RRTLDIIGQAAPSQATVLLLGESGTGKELLARHLHDLSPRAGRPLVPVNCAALPESIL
                     EAELFGYEKGAFTGAVARRDGRFAQADGGTLFLDEIGEIPLHLQVKLLRVLQEGEIEP
                     LGGKLRRVDIRLVVATNRDLRKMVSEGRFREDLFYRLNVIAVTVPPLRERLEDVPLLV
                     DHFLGRFRHKNHKNVTGISRPALDLLCRYSYPGNVRELENALERAVVLSRQPVIDVDD
                     LPQEIRCGGLQLTQSTGHGSLPSLTFPLGTPLGEIEHTVIRETLLYTKGDKKLAAQLL
                     GIATRTIYRKLDRETSDGSEQEDDDDPSR"
     gene            9846..10634
                     /locus_tag="PKL17_11985"
     CDS             9846..10634
                     /locus_tag="PKL17_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45499.1"
                     /translation="MIPHEIGVERSDERSAPHRTHVLTCSSCRAPVPLGFADQTACLR
                     CHTVIPLPEDLRSRRDNFRKVEAERRHADPLWQMVLSAHSHVYRRPLLLASMAYMAPF
                     VGIIVGYVFAYRHGQYGLAALLWLWVIGAFHLLSSRLLLHRKPLYVRQLMTAQLGSEP
                     SQPPVCRVCASPLSVPPRTGDSLHSFDCSCDHCGADNIVGDAPLVTPRPDLYSIVSAY
                     QEVFGGVVQQRMDRLYPFILLVGFLILGGLSYVATNAPHTPMAN"
     gene            complement(10641..11066)
                     /gene="ndk"
                     /locus_tag="PKL17_11990"
     CDS             complement(10641..11066)
                     /gene="ndk"
                     /locus_tag="PKL17_11990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNK45500.1"
                     /translation="MASETTLLLLKPDCIRKNLAGEVIRRLQAQGFRLRGCKMIQLND
                     DVLKEHYAHIASKPFFPEVANFMKSQPVIAIALAGDDVIAKVRDLLGPTDSKAAAKGT
                     IRGDFGEDKMVNMVHASDSPEAAAVELKRFFKDGELFNL"
     gene            11217..12356
                     /locus_tag="PKL17_11995"
     CDS             11217..12356
                     /locus_tag="PKL17_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45501.1"
                     /translation="MNRTSDSQPPAPNGPLLSPWIWLIGNRLFLVLLGFLTLTFFSAQ
                     VVPGTWRALPQNPWLDGWLRWDAGWYYELATQGYDRTVTAGQQSNTAFFPLLPLVTRL
                     VALLVGNVALAGLFVSYASCGVALLVLFRLVARRFGSTVALRTVLLLLFFPYSLYLSS
                     FHTEPLFLCLVVCSFFFAEQKSFGKAAFFAACASATRNVGVLVSIGIGVAYLDSIRFN
                     LRRLGFDALLLGICPLGLVLHMLFLWKHTGSPIQFVLSQAAPGWAGGALLAGQKNPSL
                     IAALWRFVGHPETLLFVVPSAWLLWRARHQLPLSYLVWTALTLLVSMSSPGSLGRFLI
                     VLFPLFIAAALLPWSRRSLGVYLAASGILLSINTVRQVLGMWVAG"
     gene            12423..12854
                     /locus_tag="PKL17_12000"
     CDS             12423..12854
                     /locus_tag="PKL17_12000"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK45502.1"
                     /translation="MQRFSVERVLPAPIETVWALYTDHKGWAKLLGNIRVELSPEGSP
                     DPNGVGCVRHIFALGMEMVAEEVTVFEPPTRMKYRVLRGGGPIRNHDGEVLFVAVPGG
                     TQVTWRVEFEPTIPGLGPAIKAGVRAFFVHFLTRMEAQLRT"
     gene            12851..14743
                     /locus_tag="PKL17_12005"
     CDS             12851..14743
                     /locus_tag="PKL17_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNK45503.1"
                     /translation="MKLLLISTLALFAGPILQRSLRWVGPALAALDGFVLLLVVGIVL
                     GMLLPQAYEVVGWPALPLAVVGYLAVRQIEKMHHVGGRRAHNVAVVLGLIGFAMHEFL
                     DGIALALGKGEHGSEPLLPLAIVLHRIPIGLSVWWLLRRVGTVWSVIGLCLIAIATAV
                     GYGVSAQVFAHAGGAGMGVFQSVVAGALLHVVVHEEEGRSDKFVRYEGLGGLLGLLVL
                     WLQHSELLPTARAGEASMARTLLLLTQESAPALLVAYLSAGLIGVFFPSAGVAWLGRG
                     SALAQSVRGVLFGLPLPICSCGVLPVYRSLIVRGAPAAAALSFLVAAPELGVDAVLLS
                     FPLLGLPLTVARIVCAFVVAVLAGSIVSRFLPAHSLPNAIAAESPATTPSFWQRARTA
                     YQIGYVETVDHTAPWIVLGLAIAAALEPLLRIEWVTALPKGLDVPLLALLGLPMYVCA
                     SSATPLVAVLIHKGISPGAAVAFLLTGPATNATTYGVLAQLHGRRVAVLYGVVIAALT
                     ISLGFLINHILGNSTGYPFHVGHDHSGILYPACLLLLFLLFATSFLRQGPRGFIRQLS
                     LDSSTGMSSHDGHSHHDHSHHDHSHAGHSHHDHSHHDHGSGHDHSDHAHKHSDHSHKH
                     NHRHDH"
     gene            14815..16182
                     /gene="gor"
                     /locus_tag="PKL17_12010"
     CDS             14815..16182
                     /gene="gor"
                     /locus_tag="PKL17_12010"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNK45504.1"
                     /translation="MASHGYDFDLLVIGGGSGGVRAARMSARYGARVAVAEEQRLGGT
                     CVNLGCVPKKLLVYASHFHDEFEDAAGFGFTVGTPQFDWSKLIANKDREIARLNKIYR
                     GLLEGAGVHILDGKAQLLDSHTAVIGCQSVSADKILIATGSRPHLPPLPGIEHVITSS
                     EAFSLPTLPNRIVIVGGGYIAVEFAGIFAGLGVSVTQLYRGSLFLRGFDKDLRIELAE
                     AMRNRGIALRFDSDVRAIEKTDAGFSVELLSGERIETDLVMYAAGRTPNTLGLGIDRV
                     GIDLDENGAICVDSFSRTNISNIWAVGDVTNRMNLTPVAIAEGQAFAETVFNENPQQP
                     DYENIPTAVFSQPSLATCGLSEEAARAQHAHIDVYKAHFRPMKNTLSGRNERTLMKLV
                     VERASGKVLGAHMLGPDAAEIIQGIAIAIRAGVRKVDFDRTIGIHPSAAEEFVTMRTP
                     TPTTV"
     gene            16248..16835
                     /locus_tag="PKL17_12015"
     CDS             16248..16835
                     /locus_tag="PKL17_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK45505.1"
                     /translation="MNEARYSVTWKKNGKLLVAKQPSDAELAEHAPRLADWYGDEYNR
                     YMMAGTESLHAQDVIDFWAELRAQGGQAFLLYVDGQLVGDADLRHIETDRAEFAIMIG
                     ARDTQGQGLGTLLSVLIHAFAFETLKLSAVYLTIVPHNEPGRRCYEKVGYVRDDGPLA
                     RGYAEADDDIAMRITKDALFARHPLALTEIVISRN"
BASE COUNT         3076 a         4888 c         5408 g         3663 t
ORIGIN      
        1 agcgatggcg ggctgcccaa ggttcggctg tgcgatcaga cgccaccgat gggttcgacg
       61 ctggccccgc cgccgcccgt ctacgctggg tcctgtccgc acctggtttc gggcaaaaac
      121 atcattgtgt cgtcgggcaa agcacggact ttcttgctcg cggtgccgac gacgattcgc
      181 ccggctgaga agctgccgct ggtgtttatg tggcactggc tgggcgggag cgctgactcg
      241 ttctttaaga agggggaagt gcaggcggcg gtggactctc agcgctttat ggcgatcctg
      301 cccgaggcca agggcgacat cacctggaag tggccgttcg acgtgttcgt gacagctgct
      361 cggcaagagg aagagtatcg cttcttcgac gacatgctgg cttgtgcggc ggctcagttt
      421 gcggtgaaca ccaactgtgt gtcgtcggtt ggcgtgagtg ctggtgcgct gtttaccgat
      481 cagctcgcga cggggcgaaa caagtacctg gcgtcggcga tctcgctgtc gggtggcgtc
      541 gaaggcgtgg ccaagaagtg gggcaatccg cctcggaagc tgccgatgct ggtgctgtgg
      601 ggcggtgcat cggacatctg tgcgggcctg gtcaacttcc agacggcgtc gaaggcgctg
      661 gaaacacagc tggtcaaaga cggtcacttc ttcgtcgaat gcgttcacaa ctgcgggcac
      721 gccgagccac cgcttgaagc gtcgatgatg ctgaccaagt acgcggcgtt ctgggagttt
      781 gtctttgcgc atccgtactg gctggggacc ggcgagtcgc cttacaagac gagcgggctg
      841 ccgtcgagct ttccgtcttg gtgcagtgtc ggcgctggca ctgccaagat ccgcacgggc
      901 atctgcaccg aaaagcccgg ctgctaacac ggtggctact cggctggcct tggaacgggt
      961 gacgatggcc agcttttctc ggcgtgctgc gccgcgggat gcagccgtga gacggcgtga
     1021 aggccggcca tcgcttcttt tgcggtgagc ccaatccagg gcagaaacgt cagcgtcgag
     1081 tctttgggga gtgcgcggca gacggcggcg acatcgacct cgccactgcc aggcggtaag
     1141 gcaccgatcg ggccgcatgc gtcgccgaca agatgcagcg gacccggcgc aaacgcgcgc
     1201 accgattcgc tgagcgagac aagccgcatc gtcgaagcca ggtgcgcagc cggcaggtcg
     1261 aggctgggct tgagcggcgc gccgtcaaac tcttcgagca agagccgaag ctcgatgggg
     1321 tgcggtagat cggtggcacg tcgcggaaaa ccaagcagca gcgtcagccc atgctggaca
     1381 gcgacgtttg cgagctgctc aagcgagcgc cggaccgcat ccaggtgccg tggcaaaagc
     1441 gcactgcggg cgcgcagcag ctcttcgttt ggtgtttcgt cgtctgcgag cgctcctcgc
     1501 agaaagtggg cccgcacttt gcgccacagc ctgtctatgc cggcgatctc accgagcctt
     1561 agtcgcacca tccgcgtcga cagcgcgacc gcagtttgca tcgccgcttt ggcaaccgct
     1621 accgctgtca gcgcttcttc tttgtcgaga gagcagagct ttgcgctggc tgcgtggggg
     1681 cgtcccagcg cgatcgcacc cagttctgca tcgatggaga cgacatcaat ttgcggtttg
     1741 gtctggctgc gaagtgattg aagcgactcg ataagtccca caatcggcac cgcgggcaca
     1801 tgcagcacag tggcaccttg agcaaggagc gccgacaata ctacgtccgc tgtggctgct
     1861 gtcggagaca cgccttctgg aagcacggaa agaccgagcg cacagcgggg cccgatccca
     1921 tgggatgggg tttgactttg catgacgagt caatttacac ctatgcagtc ctgtgtaggg
     1981 aaatggccgc aatctgcccg tgcacagact cgacaaaaag gataggaagc agccgatgca
     2041 atcgacattt ggagcgcaca cgcacaagcg cctggggacc ttgcttgggt ccttgggcgc
     2101 gctccttctt tcctcgcaga ctgcgagcgc acagcaagtc caagaagccg gatttacgca
     2161 gccgacctac aacatgcagt tgtttcggcc cgcgatcgac tcgaagggct atgtcacgct
     2221 gaacgcttcg caggtgcttg gacacaagga cttctcgctc gggctcgtcg gaacttgggc
     2281 gggcaagccg ctgtatctgg aggtgcctgc gggctcttcg gcgccgtttg gtccggtttc
     2341 gagtggccgc acgttcgaag tcagcaactt ggtgaccgcc cagctgcagt tcgcactcgg
     2401 tctgttcaag cacttcgaga ttgcgctcgg cattccgctg tccatcatga ccggtggccg
     2461 gcaagtctgt ccgcagggcc aggattgtca gggctatgcg ggctctggat cggcgcgtac
     2521 cgacctgacg ttttccaagg tcggcatcgc ggacctggcg attcacttca aaggtcgctt
     2581 cctgaacacc tctcggcacc cggtcggcct cggcgcgctg ctttcggtgt atctgcccgt
     2641 cgacaagtgg ggcggcggcg atgcgtacaa gcagtttatc gccgaagaca acgtcacgat
     2701 ccatccgcag atcctgctcg acaaagagtg gggtcgctcg cggcgtttcc gcaccgccat
     2761 caacgccggt gcgctcgtcc gcttcggatc gaccacgttc accgatcgag gtcgacccaa
     2821 agtcaccgat gagatgatga acaccttctg ctatccagca gacaacacgt cgacgcagtt
     2881 tgactcgggt ctgtgtggca cgggtctgtc gcgcagcgtg ggcacccagc ttacctacgg
     2941 catcggtgcc tcgctggcga ttgtgaagca gcgcttcgag gtgctcggcg agttctacgg
     3001 ctacgccgat gtgaccggca acgcgaaggc ttatccgctg gagggacttg gcgcgatcaa
     3061 gctgtacctg gccaagaagt cgttcttggt cgctggcgcg ggcggtggac ttatcggtct
     3121 gtcggattcg ggcaagatga ccggcggtcc gctgtggcgg gcgtttatcg gcttcatctt
     3181 cgagccgaac attggcgacc gagacggcga tggcatcaag gatgacgtcg acaagtgccc
     3241 cgatgatccg gaggacttcg acgacttcga agatgaggat ggctgccccg atccggacaa
     3301 cgatcgtgac ggcatcctgg acgtggatga caagtgtccg aacgagccag agaccaagaa
     3361 cggctttgaa gacgaagacg gctgcccgga tagcgtcgtg ctggaccgcg atggcgatgg
     3421 cattcccgac aacctcgaca agtgccccga tgatccggaa gacaaagacg gattcgagga
     3481 cgaggacggc tgccccgatc cggacaacga caaggacggc atcctggaca aggacgacct
     3541 gtgcccgaac gatccggaag acaaggacgg ctttgaagat ctggacggct gccccgatcc
     3601 ggacaacgac aaggacggca tcctggacaa agacgaccag tgcccgaatg agccagagac
     3661 gttcaacggc tatcaggatg aagatggctg ccccgacaag ggtcgcgtca ttgtccgcaa
     3721 gggcaagctg gagatcctcg acaagatcta cttcgagacg gacaaggaca tcatcaagcc
     3781 gatttcctac ccgatcctcg atgcaatcgc ggcgacgctg aagggcaacc cgcagattct
     3841 tgagattgaa gtccagggtc acgctgacga acgtggtgac gatgcgcaca acctcgacct
     3901 gaccgacagg cgagccaagg cggttcgcgt gtaccttatc gacaagggca tcgacggcaa
     3961 ccgtctgcag gcgcacggct acggcgaaac caagccggtc tgcatggagc acaacgaaga
     4021 ctgctggtcg cgcaaccgcc gcgtcgagtt cgtcatcctg aagcgctctg acggcgacac
     4081 caaggactag tcagcaggcc gaagtgaccc tggcgcgcgg cgcggggtca cccttccttt
     4141 cctcttcgtc tcaccttcct aaagtcccgg cacaagcagc gccaaaaacg taccgagcag
     4201 actcgctgca aacagcgcgg actccctgct gtcgtccgtg tggcgcgaca gcttttccat
     4261 ggccgtcacc gcgatgatca gcggcagaag cagtcccagc ggaagccgca gaagtccgcg
     4321 cagcggctct gccggaaacg cggcatagca acacactgca gcgatttcag cgagcagcgc
     4381 ggcgatgaat acaccccaca gcggatgccg cagagaaagc cagcgggcca ccatcgtcac
     4441 catcaaagcg gacaagatca gcgacagcac gatcagcaga tcggtgcgct gaaacggcag
     4501 cagccaccac agggttcggt agctaccaac ggcgagcgca caccacacaa tggcggcggc
     4561 caaaagtcca cgcaggggtc cggggatgct cgttatctgt ctcacacagc gtgcaaggat
     4621 cactccgatg cacatcatga cgacgagcga tccccaggcc agtgcaatcc gcatgctgat
     4681 gcgcagcgtc gcccctttgg gtttggccag cggcagtgcg cgatcgatgg cggtttcgat
     4741 gacactggga gtccatggtt cgagcacatg ggacaccggc aagtagaacg ctgccgacag
     4801 tccaagctgc tggtgaagcc gtccgtagat catgcggcgg tcttcacaag gtacggactg
     4861 acctatcgcg acgcagtgac tgcaagaaaa cgcgctgcca cagcccaagt acgcgcccat
     4921 ggaatggccg acgaaatagg tgtgctcggc actttgaccc agcgctcgtt cgacgtggag
     4981 cgcggctcgc atggccgaga tggtgttgag tatcggaacg ggacttgatg cgccgtggcc
     5041 cagctcatcg attgttacgc agtcggcacc gcggctgacg gcttcatagc aaagtccgag
     5101 cagggtttct ttggtcgcga gtccgccgtg tgcggcaatc agcagcgggc ggggctttgt
     5161 gccttccgca ctgcgaaaca gcagcccttt tgccacatgc tgggaatcga cgcggacggt
     5221 gacggactca ccgagcggcg cgcccgctga ccaaagccag acccggagcc caagccacac
     5281 cagaaagagg acagcggagc caagtaaggg agtcgcaatc gcacgcttca tcgccggtca
     5341 aggatagccg gacaacgaag cggacgcccg gcgcgaaacg gacgcttgtc tgcgtggggt
     5401 tttagcgggt gccgatgtga aggtcgccgc tcatgctgtc gagctcgatg ctgccgacgc
     5461 cttggcccag cttcgcgatc cactcggtgc cactccgcgg cgggcctttg cgctccatcg
     5521 tcagctcgcg gatgccgagc agctcgccgc tcttggtctt gagcttggcg gaaagggagc
     5581 tgtcgcgatc gttttgaatc gtcacgtttc cggacgtcgt ctgggcggtc actttgcagc
     5641 ttgcgatgcg gcacggaccg gccaatgtca tgtcaccgga aatcgagtgg aagcgcaccg
     5701 ggcttacgat ttcgccgccc atgtgtagct cgcccgatgt ggtcttcgcg tcgacggtgc
     5761 cgctgatatc gtgaagctcg atgtcgccgc tgaccgattg aacgtagagc gacctggcct
     5821 gtttgacggc aatatcaccc gaagcggtca cgacgctgac atccgacttc aggtcgtgga
     5881 cgcgcacgtc tccagacgcg gtcttcacat cgactttgct gccttcggga agctgcaagc
     5941 ggagtgcgcc atttggcagg gttgtgctgc catcgaagtc gagccgccag cttccctggc
     6001 cgcttgcggg cggaatcagc ttcacggcgc gcacgccaga ctcatcgagc gatagcttgg
     6061 cttggtcttt ggcaccttgg ctgacttcca catcggcagc atcggtgaca atttccagac
     6121 tgatcggcgc gctgactttg acttgggtgg tcagcgtgtc ttccggttcc gctgcatcgg
     6181 catcgttgtc ttcctctccg gcgccatcgt gcagctcgga cagatcgccc atggcagaga
     6241 gcggattggg aatgttcagc ttttcagtca cagtcacatt cccatggttc gctttgcgca
     6301 gaggcgacag cgggctggcg gcgaccacac cgaagattgc aattgcagca gcaccgacgg
     6361 cgtaccactg ggctcgctgg tttgcaaagt tgcgtcccaa gatgcgcgat agaccccgcg
     6421 gggcttgttc cttttgacca atgcgcgctg cgatcttcgc ttcgacttgc gcccagactt
     6481 ctgaaggtgg gacgcctcgc gcacgctggg caaacagttc tttttccgcg cgtagccacg
     6541 ccacttcacg actgcactgc ggacattcat cgctgtggac cgaaatcaca atcgaacggt
     6601 cttcatcgag cgttccagcg acgagtgctt caagatccga aaccgaagga tgggtactca
     6661 ctttgtgcct cccaaatagc gccagagctg cgtgcgcagc tgcaaccggg cgcggtgtat
     6721 ttcgtttttc actttggcca ctgaccagcc catcacgtcc gcgatgtctt cataggaaag
     6781 cccgtgatca agtcgcaaaa gcagcgcact ttttcgcggc tcgccgacgt gggtgagcgc
     6841 ttcgctgagc acccgatctg cttcggcgag aagaagcact gcatcgggcg tgggcgcgtc
     6901 atctaccggc tcgcattctt cgtttggttc gtctaagcgc cgcgccgatt gcttctgcgt
     6961 gcggcagtgt tccaaaaaga cattgcgagc gattccaaac agccaagaag acagacgggc
     7021 gctttcacga agctgggcaa gcgagtcgaa ggcgcgcaga aacgtctctt gggtcgcatc
     7081 atcagcggct gcatcatcgc gaagcagatc accaaggtag cgacgcacgc ctggactgtg
     7141 gtgttggaac agtgcacgga atgctgcgct gtctcctttt tgggcgcgga ccacaagcgg
     7201 atcgaaaatg ctggtgactg cgcgttcttc ttccacgggg ccagccagtg gatgtgaagt
     7261 gtgaagcagc gtgagcgccc gttttcccgt catctcatcc gtgagtgaag atgcgggcgc
     7321 tttggtttca gctttttttt tcgatcgtga aaaaaatcga aacggcaagg cagctggctt
     7381 ggttttgggg gctagggcga agacagaaac ggctcaagga cgtgaagcgc cgcgtcgtgg
     7441 agctgaccat ttgagccaac cagtccgtgg tgatgtccga gatccagatc ggaatagcgc
     7501 agcggcttgc cacgcatgtc ggtcagctta ccgccggctt catgcaggat gacttcgggc
     7561 gcggcggtgt cccacagctt gcaacgtccg gcggggttca tatacaggtc gcgggcggcg
     7621 gcggcgatca ggcacagctt cacaccgaca gatccgacct ggacttcatc gcgaatgccg
     7681 gctcgatctc ggacgtggtt catcagatcg ggacgcgagg acgcagacga cacaagccgt
     7741 gcctcggcga tttcgcttat gggcgacacc tggattcgtt gcggcggctc gtcttcgatg
     7801 atctcgaaag cgccgcggcc ccgctcggca agccacagcc gcgcggctgc cggttggtac
     7861 accgcgccga ggatgggaat ccccgacgca accaggccaa tcatcacgga ataaccggcg
     7921 cggccagcga tgaagtcttt ggtgccatca atgggatcga cgagccaaaa ccgcggtgcc
     7981 gatagacgag cgccatcgtc gggtcgttct tctgaaagca cgacgtcact tggatacgcc
     8041 gcggcgatgc gggcgatgca cagctcactg acggcgcgat cggcttcggt gacgggctct
     8101 tcggcgtctt tccattcgac gggcaagctg ctgggtgagc tgcccggcaa gtgccatgca
     8161 acaaagtaac ggtgggccag cgcgcccgct tccaaaatga tggggacaat ccgccgcgtt
     8221 tcttctgaaa aaccggcggg gatcatgctg tctcggaagt tcacgcccgc agggtatcag
     8281 ggttacggct tagcgcgacg ggtcgtcgtc atcctcttgc tcgctgccgt cgctggtttc
     8341 acggtctagc ttgcgataga tggtgcgggt ggcgatgcca agcagctgcg ccgcgagctt
     8401 tttgtcacct ttggtgtaaa ggagcgtctc gcgaatgacg gtgtgctcga tttcgccgag
     8461 cggcgtgcca agcggaaagg tcagactcgg cagcgagccg tggcccgtgc tttgggtgag
     8521 ctgaagcccg ccgcagcgaa tctcttgggg caggtcgtcg acatcaatca ccggctgacg
     8581 tgacaaaacg accgcacgct ccagcgcatt ttccagctcc cgcacgttgc cggggtagct
     8641 gtagcggcac aaaaggtcga gcgctgggcg gctgatgccc gtgacgttct tgtggttttt
     8701 gtgacggaag cgacccagga agtgatcgac gagcagcggc acatcttcca accgttcgcg
     8761 cagtggtggt accgtcactg cgataacatt gagtcgataa aacaggtctt cgcggaagcg
     8821 gccttcgctg accatcttgc gcaggtcgcg gtttgtggca acgaccaagc ggatgtcgac
     8881 acggcgcagc ttgccgccga gcggctcgat ttcaccttcc tgcagcacgc gcagcagctt
     8941 gacttgcagg tggagcggaa tttcgccgat ctcatcgaga aacagcgtgc cgccatcggc
     9001 ttgcgcaaat cggccatccc gtctggcaac cgcgccggtg aatgcacctt tttcgtaacc
     9061 aaacagctcg gcttcgagga tgctttccgg cagcgcggcg cagttgaccg gcacgagcgg
     9121 acgaccggcg cgcggcgaca gatcgtgaag gtggcgcgcg agcagttcct tgccagtgcc
     9181 cgattcaccc agcagaagca cggttgcttg cgatggggcg gcctggccga tgatgtcgag
     9241 cgtgcgacgc attgccatgg ctccaccaac gatggtgcga cgctcggctg ccgccagttt
     9301 ctgtcgcagc gagcgatttt ccagcaccag cgtttgtcgt tcgagcgctt ttccaacgac
     9361 acgaagcaca tgggcacgtt tgagcggctt ggtgatgaag tcgtaggcac cgtctttcat
     9421 ggcctcgacc gccgtttcga tggtgccgta agcggtcatc aaaatgacct ctgtttccgg
     9481 cgacagcttg cgtccggctt tgagcagatc gagccccgag atacccggca tcatcagatc
     9541 ggtaagcacc acatcgatgc gctgcttgcg caagatttct agcgcatctt ttccgtcgct
     9601 acaggtgatg acttgcagct cgctgccact cgattcggaa tcggtgtggg gcgggacaaa
     9661 gcgcgcaaag gtgcgctcga gggagtcgag gttggaagtc tcatcatcga cgataagtag
     9721 cgtcttcatg ggctaggctc gcagcaggcg atgggcatct tacgccaagc ctcgattctg
     9781 ctgctttctt gctggtacgg caaacaggac ggctcgggcg gcgcaaactc tgctagtctg
     9841 tggccgtgat cccgcacgag attggcgtcg agagaagcga tgagcgctcg gctccgcata
     9901 ggacccacgt cctgacttgc tcaagctgcc gcgcgcccgt gccgcttggt ttcgccgatc
     9961 agaccgcgtg tctgcgctgt cacacggtga ttccgctgcc cgaagacttg cggagccgcc
    10021 gggacaactt tcgcaaagtc gaagcagagc gacggcacgc tgatccactg tggcagatgg
    10081 tgctttcggc gcattcacat gtctatcggc gaccgctttt gctcgcgtcg atggcgtaca
    10141 tggccccgtt tgtcggaatc atcgtcggat acgtgtttgc gtatcggcac ggacagtacg
    10201 gtctggcggc gcttttgtgg ctgtgggtga tcggcgcgtt tcatcttctg tcatcgcggc
    10261 tgctgctgca tcgcaagccg ctttacgtgc gccagctgat gacggcgcag cttggcagcg
    10321 agccttcgca accgccggtc tgtcgggtct gtgcatcccc gctttccgtg ccgccacgga
    10381 ccggggattc gctgcattca tttgactgta gctgtgatca ctgcggagcc gacaacatcg
    10441 tcggtgatgc gccgcttgtg acgccgcggc ctgatcttta cagcattgtg agcgcgtacc
    10501 aggaagtctt tggcggggtg gtgcagcagc gaatggatcg tctgtatccg ttcattctgc
    10561 tcgtcggctt cctgattttg ggtgggctgt cctatgtggc gacaaacgcg ccgcatacgc
    10621 cgatggcaaa ctagcgtcga ttacaggttg aacagctcgc cgtctttgaa gaagcgcttg
    10681 agctcgacgg cggcggcttc gggtgagtcc gaagcgtgaa ccatgttcac catcttgtct
    10741 tcgccgaagt caccacggat ggtgcccttg gctgcggcct tgctgtcggt cgggccgagc
    10801 aggtcgcgga ctttggcaat cacatcgtcg cccgcgagcg caatcgcaat caccggctgg
    10861 ctcttcatga agtttgccac ttcggggaaa aatggcttgc tggcgatgtg cgcgtagtgc
    10921 tctttgagca cgtcatcatt gagctggatc atcttgcagc cgcgcagccg gaagccctgc
    10981 gcttgcaggc ggcgaatcac ctcgcctgcg aggtttttgc ggatgcagtc gggttttaaa
    11041 agcaggagcg tcgtttcaga agccatgacc attccctttc gtaagccttg ctgtgtctta
    11101 acgggcgcgc aggttgccct agtttcgccc cacacgtcaa gcgctggaga tgtctgtcac
    11161 catcgcggcg gcctctggtt tgactttggt cggctgtttc ggacacgctg cgcgcgatga
    11221 acaggacttc cgattcgcag ccgcctgcgc caaatggtcc acttttgtcg ccgtggattt
    11281 ggctgattgg gaatcgtctg tttcttgtgt tgctcggctt tttgacgctg acgttttttt
    11341 ccgcgcaggt tgtgccgggt acgtggcgag cgctgccgca gaatccttgg ctcgacggct
    11401 ggctgcgctg ggacgcgggt tggtactacg agcttgcgac ccaagggtac gaccgcacgg
    11461 tgacggctgg acagcagagc aacaccgcgt tttttcctct cttgccgctg gtcacgcgct
    11521 tggttgctct gctggttggg aatgttgcgc tggctggtct ttttgtttcg tatgcatcct
    11581 gcggggtggc tctccttgtg ctgtttcgtt tggtggcgcg gcgctttgga tcgaccgtcg
    11641 ctctgcggac agtccttttg ctcctgttct ttccctactc gctttatctt tcgtcgtttc
    11701 ataccgagcc gctgtttctg tgcttggtcg tctgttcgtt tttctttgcc gagcagaagt
    11761 cgtttgggaa agcggcgttt tttgcggcct gcgcaagtgc cacgcgcaac gtcggcgtgc
    11821 ttgtgtcgat tgggattggc gttgcgtacc tggactcgat ccgtttcaat ctgcgcaggc
    11881 ttggctttga cgcgctgctg cttgggatct gtccgctcgg acttgtgctg cacatgctgt
    11941 ttctgtggaa gcacacgggg tcgccgatcc agtttgtgtt aagccaagcg gcgcccggct
    12001 gggctggtgg tgcgctgctt gcgggacaga agaatccgtc gctgatcgcc gcgctgtggc
    12061 gctttgttgg ccatcccgaa acgctgctat ttgtcgttcc ctcggcgtgg ctcctgtggc
    12121 gagcgcggca ccaactgccg ctttcctatc tggtctggac tgcgctgaca ctgcttgttt
    12181 cgatgagcag tccgggctcg cttgggcgtt tcctgattgt gctgtttccg ctgttcatcg
    12241 cggcggcgct tttgccctgg tcgcgtcgca gcttgggcgt ctatctggcg gcgtcgggga
    12301 ttcttttgtc gataaacaca gtgcgtcagg tgctcggcat gtgggtcgcg ggctgatcgt
    12361 caccgggcaa agccattgca agcaagatgc ggatctgcgt agtgtcgctc gggtactgat
    12421 tcatgcagcg gttttccgta gagcgagttc ttcctgcgcc gatcgaaacg gtctgggcgc
    12481 tttataccga tcacaagggc tgggccaagc tgcttggcaa catccgggtt gagctttccc
    12541 ctgaaggttc gccggacccc aacggcgtag gctgcgtccg tcacattttt gcgctcggga
    12601 tggaaatggt ggcggaagaa gtgacggtgt tcgagccgcc cacgcggatg aagtatcgcg
    12661 tcttgcgcgg tggtggaccg attcgcaacc acgacggcga ggtgctgttt gtcgctgtac
    12721 cgggcggcac tcaggtgacg tggcgggtag aatttgaacc aaccattccg gggctcgggc
    12781 ccgctatcaa agcaggcgtt cgggcctttt tcgtgcactt tttgacgcgc atggaagcgc
    12841 aactgcggac atgaaactgc ttttgatttc gacgctggcg ctgtttgccg gtcccatctt
    12901 gcaacgctcc cttcgctggg tcggaccagc gcttgctgcg ctcgatggct ttgtgctgct
    12961 tttggtggtg ggcatcgtgc ttggcatgct gctgccacaa gcctatgaag tggtcggctg
    13021 gccggcgctg ccgctggctg tcgtgggcta tctggcggtg cggcagatcg aaaagatgca
    13081 tcacgtcggc ggacggcggg cgcacaacgt cgcggtggtg ctggggctta tcggcttcgc
    13141 catgcacgag tttttggacg gaatcgcgct ggcgctggga aagggcgagc atggttcaga
    13201 gccgcttttg ccgctggcga ttgtgctgca tcggattccc atcgggctgt ctgtgtggtg
    13261 gcttctgcga cgggttggca ctgtgtggag cgtgataggg ctttgcctga ttgcgattgc
    13321 gacggcagta gggtacggag tgtccgctca ggtgtttgcg cacgctgggg gcgccgggat
    13381 gggcgtgttc caaagtgtcg tggcgggcgc gctgctgcac gtcgtggtgc acgaagaaga
    13441 gggccggtcg gacaagtttg ttcgctacga aggactgggc gggctgttgg gtctgctggt
    13501 tctgtggctt cagcacagtg agcttttgcc gacggcgcga gcaggggaag cgagcatggc
    13561 gcggacgctg cttttgctta cgcaagagag tgcgccggcc cttctggttg cctatctgtc
    13621 agcgggactt atcggggtgt tttttccttc tgcgggagtg gcgtggcttg ggcgtggttc
    13681 ggcgcttgcg cagtctgtgc gcggcgtgct gtttggcttg ccgctgccga tctgttcttg
    13741 cggtgtgctc ccggtctatc gcagcttgat tgtgcgcggc gctccggctg ctgcggcgct
    13801 gtcgtttttg gtggcggcgc ccgaattggg ggttgatgca gtgctgcttt cgtttccgct
    13861 tctgggtctg ccgctgacgg tggcgcggat tgtctgcgcg tttgtggtcg cggtgctggc
    13921 gggaagcatt gtcagccgct ttcttcctgc acattcactt ccgaatgcca tcgctgctga
    13981 gtcacctgca accactccgt ccttttggca gcgtgcgcgc accgcgtatc agattggtta
    14041 tgtcgaaacg gtggatcaca ccgcaccgtg gattgtgctc gggttggcga ttgctgcggc
    14101 gctcgagccg ctcttgcgga ttgaatgggt gaccgcactg cccaagggac ttgatgtgcc
    14161 gctgctcgct ctgcttggat tgccgatgta tgtctgtgca tcgtcggcga caccgcttgt
    14221 cgcggtgctg attcacaaag gcatttcacc cggtgctgcg gtggctttcc ttttgactgg
    14281 gccggcgacg aacgcgacca cgtatggcgt gcttgcccag cttcatggga gacgggttgc
    14341 ggtgctgtac ggtgtggtga ttgctgcgct gaccatctcg cttggctttt tgataaacca
    14401 cattctggga aacagcacag gctatccgtt ccacgttgga catgatcact ctggcatcct
    14461 gtatccggcg tgtctgctgc tgctgtttct tttgtttgcc acatcgtttc tgcgacaagg
    14521 tccgcgcggt ttcatacgcc agctctctct ggattcatcg acgggcatgt catcccatga
    14581 tggccattcc catcacgacc attcccatca tgatcactcc cacgcgggtc attcccatca
    14641 tgaccactcc catcacgatc atgggtccgg tcacgatcac tccgatcacg cgcacaaaca
    14701 ctccgatcac tcgcacaaac acaatcaccg gcacgaccac taaccacggt cgcgacggaa
    14761 ctttgccgca cgatttgccg tgatttgtgc ggcggttttt gcttggatgg acacatggca
    14821 agtcatgggt acgactttga tctgctggtg attggcggcg gttcgggtgg tgtgcgggcg
    14881 gcgcggatgt cggctcgcta tggcgcaagg gttgccgtcg ccgaggagca gcgtctgggc
    14941 ggaacgtgcg taaacttggg ctgcgttccc aaaaagctcc tggtgtatgc gtcacacttc
    15001 cacgatgagt ttgaagacgc cgccggtttt ggctttacgg tgggaacgcc gcagtttgac
    15061 tggtccaagc tgattgcaaa caaagaccgc gaaattgcgc ggctgaacaa aatctatcgc
    15121 ggtcttttgg aaggcgcggg cgtccacatc ctcgacggga aagcccagct tctggattcc
    15181 cataccgcgg tgattggctg ccaatcggtg agcgcggaca agatcctgat tgcgaccgga
    15241 agtcgtccac acctgccgcc gctgccgggc atcgagcatg tgattacgtc gagcgaagcg
    15301 ttttccctgc ccacgcttcc caatcggatt gtgatcgttg ggggcggcta cattgccgtc
    15361 gagtttgccg gaatttttgc cgggctcggg gtctctgtga cgcagcttta tcgcggctcg
    15421 ctgtttctgc gcggatttga caaagacctg cggattgagc ttgcggaagc gatgcggaat
    15481 cgcggcatcg cgctgcgttt tgacagcgat gtgcgcgcga ttgaaaaaac agacgcggga
    15541 ttttccgtcg agcttttgtc tggtgaacgg atcgagaccg atctggtgat gtatgccgcc
    15601 ggtcgcacgc ccaacacgtt gggacttggc atcgatcggg taggaatcga tctggatgaa
    15661 aacggcgcca tctgtgtgga ttccttttcg cgcacaaaca tcagcaacat ctgggcggtc
    15721 ggtgatgtga caaatcgcat gaacctgact ccggtggcga ttgccgaagg ccaggcgttt
    15781 gctgaaacgg tgtttaacga aaatccgcag caacccgatt atgaaaacat cccgaccgcg
    15841 gtgttcagtc agccaagtct tgccacttgt ggactgagcg aagaagcggc ccgcgcgcag
    15901 catgcccaca tcgacgtgta caaagcgcac ttccgaccga tgaaaaacac gctgtctggt
    15961 cggaatgagc gcacgctgat gaagctggtg gtggaacgcg caagtggcaa agtgctgggc
    16021 gctcatatgc tcggccccga cgctgctgag atcattcagg gcattgcgat tgcgattcgg
    16081 gcaggggtcc gcaaggtcga ctttgatcgc acgatcggaa tccatccgag cgctgccgaa
    16141 gagtttgtca ccatgcggac gccgactccc acgaccgtgt gatgcgcctt ccgataggtg
    16201 acgctggtgg gcccttgggc aacatgtctt cacaagagtg caggtctgtg aacgaagcgc
    16261 gatattccgt aacgtggaaa aaaaacggca agctgctcgt cgccaagcag ccatctgacg
    16321 cggagctagc ggagcatgca ccgcggctcg cggattggta tggcgatgag tacaaccgat
    16381 acatgatggc gggaacagag tcgctgcacg cgcaggatgt catcgacttc tgggcggagc
    16441 tgcgggcgca gggcggccag gcttttttgc tctacgtcga tggtcagctc gtcggcgatg
    16501 cggacctgcg acacatcgaa acggatcgcg cagagtttgc gatcatgatt ggcgcgcgtg
    16561 acacccaagg ccaaggactg ggcacgctgc tttcggtgct gattcatgcg ttcgcatttg
    16621 aaacgctgaa gctgtctgcg gtgtacttga cgattgttcc gcacaacgaa ccggggcggc
    16681 gctgttacga aaaggttggc tacgttcgcg acgacggacc gcttgcgcgc gggtacgccg
    16741 aagcggacga tgacatcgcg atgcggatca cgaaggacgc actttttgca cgacatccgc
    16801 ttgcgctgac cgagatcgtg atatctcgca actgattccg cttcacactg ggtttggctt
    16861 ttccaaaaag gagcttctgc gatgacgtac cgacttttgg gttcgctgac ttccccgttt
    16921 gtccgtcggc tgcgacttta catggccgac attccctatg aatttgaaat cgtcaactac
    16981 ttggaaagcg gtcaggatgc gcggctgtcc gcggccaatc cgatcaagcg gattc
//
LOCUS       DAORGN010000098        16979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1369_length_16979_cov_5.692803, whole genome shotgun sequence.
ACCESSION   DAORGN010000098 DAORGN010000000
VERSION     DAORGN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16979
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1369_length_16979_cov_5.692803"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..857)
                     /locus_tag="PKL17_12020"
     CDS             complement(9..857)
                     /locus_tag="PKL17_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45506.1"
                     /translation="MRNRRWMCALGLFFGLGVSETAWAKDPSELHQDALRAFTEARYE
                     EALRLFKEAYDATNTPEMLVRIGQTQLKLGRKAEAMDACQSYLSLTADRPEPMYRGFA
                     EQCVTDAKSGLFPASSSARPTPAPTAPAAATNRKPPLIVEEDDPPTPAPRPLLATTPP
                     PKPVISPPAPPPPAVPPGLASSMTTTPSGASLTAVYDYCLRQQREGRTDSAQRCYEEL
                     LPNALRSSGMQEADITLVMSQIRRYPHPAAAVPWSMQKHTVEKRNTGLWAAGLTLWLS
                     ALVPAV"
     gene            complement(945..1385)
                     /locus_tag="PKL17_12025"
     CDS             complement(945..1385)
                     /locus_tag="PKL17_12025"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNK45507.1"
                     /translation="MNPADVFELVTAKIPMVQKLGLQAVDLSPSAARILLPFDSQLGN
                     HGGTVHVGAQFVAAETAALSAGLLFLNGMDVTCHSKGCELRFRKPARGDLWASAQLGV
                     SDPSELKQRLVAEGKVDVAVLVDVTDTNGERVTEGTVTLSLRRL"
     gene            complement(1496..2206)
                     /locus_tag="PKL17_12030"
     CDS             complement(1496..2206)
                     /locus_tag="PKL17_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNK45508.1"
                     /translation="MNEALIIIPTYNERDNLPTLCDQVLAALPTADLLIVDDNSPDGT
                     GQLADEMAKANPRIHVLHRSGKLGLGTAYIAGFRYALSKHYQYIFEMDADFSHDPVYL
                     PALLAAAKDGAGVVIGSRRVPGGGTENWGLSRQLISAGGSLYARTILGLGVRDLTSGF
                     KCFRREVLEAIDLDAVRSNGYSFQIEMTYRAVRKGYSVAEVPIIFIDRRAGQSKMSSK
                     IFAEAMGMVWRLRFASGI"
     gene            2354..2426
                     /locus_tag="PKL17_12035"
     tRNA            2354..2426
                     /locus_tag="PKL17_12035"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2387..2389,aa:Asn,seq:gtt)
     gene            complement(2444..3286)
                     /locus_tag="PKL17_12040"
     CDS             complement(2444..3286)
                     /locus_tag="PKL17_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK45509.1"
                     /translation="MISVRPSILVTGSSDGIGLETAKQLARHGADVIVHGRSADRVRA
                     AQKAVEAASGRPQPAPVIGDLSSLAGATKLAAELATAATFPRVLVNNAGVFCKTPAVS
                     EDGIELTMAVNHFGHFALTMALLADPRCQLRRIISVSSIAHNRGHIRLDDITLRKRRF
                     DGYEMYAASKLANVLFAVELAERLRGKVAVNALHPGVVSTKLLQAGFGMDGPDSVSDG
                     AATSVMLALDPAYEHISGQYFAARRPAAMNPVAHDRAFCRAFFDATQQVVSDAQNKKG
                     LPVS"
     gene            3484..4182
                     /locus_tag="PKL17_12045"
     CDS             3484..4182
                     /locus_tag="PKL17_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNK45510.1"
                     /translation="MITIRRGVERGHKNHGWLDTFHTFSFSSYYDPAHMGFRNLRVIN
                     EDRVAPGQGFGTHPHNDMEIISYVVEGTLQHRDSMGNGSLIRPHDVQRMSAGTGVTHS
                     EFNGSQTEPVHFLQIWLLPSQRGIKPGYEQKTFSRQEKQGRLRLVASTDGRDGSVQIH
                     TDAALYATVLSAGQTVEHAFSSGRHGYMQIVRGAVLANGQMLGAGDGAALSGEQSVVI
                     EGKDDSEVLLFDLP"
     gene            complement(4203..5603)
                     /locus_tag="PKL17_12050"
     CDS             complement(4203..5603)
                     /locus_tag="PKL17_12050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45511.1"
                     /translation="MSQDKPGGAFGGPKASQETLQLPADTDERNVPFEIYGAEPSSVP
                     NHISPEQLIAGRYLIEGSLGEGGMGRIFKVRHRDLDKLFALKIIHDDLAKQPHIRDAF
                     YREARMAAAMSHPNIIGVFDFGVDAHHGAFIVMDYLYGETLAERLNRERVLSTKAACD
                     IMLQCAEGLNYMHGRGVIHCDIKPENIFLCASATGDDHRRNDVRLLDFGLASRPDVDP
                     RELVAATTVAGTPTYMAPERIRVQTPTPASDVYALGVLFYECLTGRPPFGGRLQDILY
                     GHLRETPIPPGRLVETQPPLSKSLEDLVMRALAKDPGQRYQQAADFAFELRQIMNSLG
                     MRRRMDDTAKPVSPRADLCEALVDACPLPLFVASPTCELLFGNFALGLLLGVPPEELR
                     GKLSTTKIGQWCPRIEREVRHVLTKQRPSRHALLIENRVGEARGGWLTLAPYRRDGAV
                     VGVCGVVAVMGDIESA"
     gene            5772..8390
                     /locus_tag="PKL17_12055"
     CDS             5772..8390
                     /locus_tag="PKL17_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNK45512.1"
                     /translation="MSDETPSEKNPPRGMLTKLLGAPWLLLFLTLAVGAVGLFGVSKL
                     KSEEDLLVFLPEGDADVTVFRDVASRFGALRVALIGVEPKPGQKLFSPDMLSRIDRLS
                     TQLKNTTGVDRVVSPTTINDLVPIEGGVDLTLLVPQPIPTDQATLDKVKARALSQPTI
                     RGNVVSADGEAGLIMVFVGDGVPTRQLAEKAQALARSELSPVASLYFGGAPFAGQAIY
                     DDTQRDVQRLVPIALLLFLFVVLSAFRDFLSVLLTVGTVGLSVLLALGLLGLHGDKFT
                     VVMGTLPLVLFASGSQYAIHILGRYYLIRSAEPSRNALSAAREALTVAGPPVVVAALN
                     CCVGFLSFLVMNISAMRAFGVACCVGIIACCAFSLTVAPSMVAHFQADRPATEASFAR
                     LGSFLSGLFSWVRRRRAAIAIGVVALSCVCGYFMTRVTVRMEPKAFFRPGSEPALAQK
                     FLDDKFGGAAFVQVLLDGDMTDPRSLHEVRRLSAFARSLPGVTQVQHIVQPLAMVGDS
                     MAGLRGLPQNSQQVVALLFFLEGEPSLRTMLSPDRKSVLVHVRVLGDAAQVVSELRRY
                     VSSRLPRSPQRQSLAELSEELSWLLPPAVRNERTLAISQTVAELEKNGLALAGPAKTD
                     KPESPPAGDVNADGEEQARMSRAQAEAKRRLLAVLGAEKPDAQGQRLGRPADDVEAWV
                     EWVAAAILQPPVDAPSTTLQARLTGEPMLDQAFSRAVDRNQWASLAIALGGVLIVLLF
                     AQRSLVGAILSTLPAVMALGVVFGILGLLGKPIDLGTSLVGSMVTSSGADFAMHYIWY
                     LRRQPASQVVPRVGPVILTTAIVLGLAMGVMMLGASPPLRLFGALAAAGMLLSATFTF
                     LLVPALIGKLPEEPTS"
     gene            8424..9233
                     /locus_tag="PKL17_12060"
     CDS             8424..9233
                     /locus_tag="PKL17_12060"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNK45513.1"
                     /translation="MKSTFTKLLALGTLSLGLFAAPTTFAQTPAAPSGSAPVVQEETA
                     ESLLSRIDKQNAAFSDAFFHFKMVVKAANGSVEREVEFTTKQKGNQKRLVRFLSPGDI
                     KGMGFLLEKPGEMYAKLPAFGNRIRRLGTSQMNQSFMGSDLTSEDMSAIDYAPSYAPT
                     NKSADGALTVLDLQLRAGKSSEFPRLKMWVDSAKATITKIEYYDNAGKKLRTCERSDF
                     KQDGEAHYSPGKMTYIDHRRGDHKTDMILVKSTLNNGYKDDEFTQRALQQD"
     gene            9279..10895
                     /locus_tag="PKL17_12065"
     CDS             9279..10895
                     /locus_tag="PKL17_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45514.1"
                     /translation="MALPLSVQPARADVDLKFSGSISSDMRFRIQGQDRALPTPTPSQ
                     WTLLKNGFSRNENRIRAQLGVKLGQKVRGVADAELMLYGFSDLRDIDSATLRDRVDPY
                     YLEVHAAYIDVYNLFGDVIPRFDLRLGRQTVPWGAADKFNPISNLNTLDLSDPLLFGR
                     ALGNNMVRADWNPVGDLQFTAVLVPIFRPAQLPRTAPFALLDPLRPAPVLDQGVRTAL
                     DNERAVYVPVRDPVTGVLLPAGRTLTVQADVLAPEVSAANMQFGGRMAWKLLGQDMGL
                     SYYHGRFGIPVPAWSVSTGTLDNNVTLTQLVYPRVDVAGLEMAGSIERLGGMGYWIEA
                     AVNFPGEVEMGLYDAITSPNAPRAMEYLPGPDGRYYRNTLPVGARGARPLVIDKTPFP
                     KATVGADYAFGGHVYVNIQYVHGFIDEFGAGRVARPRKDAIDVSAQPRVESRIGDYVV
                     AGLDVKLLNERLLMRLFGVLKLPSIDLQSKLWDDWAPTGVLFPQIIWNVFDGTELMVG
                     SFVMLGDRSTKFGDPAAGATEVFVRGKVSF"
     gene            10978..11781
                     /locus_tag="PKL17_12070"
     CDS             10978..11781
                     /locus_tag="PKL17_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNK45515.1"
                     /translation="MAKMSVRRKLAIASYSPPREGNIYGKLVVDATPGLGYLEDVRKR
                     TGEKVSFTHLVGRAVAEALRQAPTLNGRIVFGEFEPFPTVDIAFLVALEDGQDLARAK
                     VCDVDKKSIAEIAQELSRKSGKLRASADPAYEKSKQAIKMMPTWLLRPMLDVTGYLAS
                     SLGLSIPALGVEPFPFGSCIVTSVGMFGLEEGFVPPTPFTRVPVYILVGAVKERPAVV
                     DGKVTIQPQLTLTATIDHRFIDGAQLAVLAKVVRSILENPWQLDRKDKS"
     gene            complement(11803..12228)
                     /locus_tag="PKL17_12075"
     CDS             complement(11803..12228)
                     /locus_tag="PKL17_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNK45516.1"
                     /translation="MGDWLTYAGHRTLVTDDLVVVEPHGHMSPPHISKLLNEILARVI
                     AAHGLAYVLLDARDGTPADAESRRIISDWVRAHPGKTIFAVFGASRTASAVAMLLLNA
                     IRLVSGRRLQLRMFDGEAEARSWLLAQRERRKNGQPVDV"
     gene            12322..12834
                     /locus_tag="PKL17_12080"
     CDS             12322..12834
                     /locus_tag="PKL17_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45517.1"
                     /translation="MNRRRFFQLSALGTIGLGAALWVGEHTLGRGVPAKLDQHMEGTV
                     LSSSESQTLRAVSLRILDGAVPDPAGDGGAAICRFVDRYLLELPRPLQRDVRALLHLV
                     ELFPLLSLRMARFSHLRGDEQDAMLADWQDSRIGLFRQGFQALKSLCCLAHYQDARSF
                     AAIGYSGPLV"
     gene            12874..13362
                     /locus_tag="PKL17_12085"
     CDS             12874..13362
                     /locus_tag="PKL17_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNK45518.1"
                     /translation="MRAKLLPSYWLLLSVFVCFAGFVGFRRPKSLSLRPLPLCPCDIP
                     VALPAGMIRCLSTQEALDWNVQAGDVPPLSASGTRLFGPPNRMSGERQLALGLPIDPN
                     EASQADLEALPDVGPALAQAIVTARRQRRFLTEVDLLAVRGLGPKRLAKLRPYLQLRK
                     TR"
     gene            13412..14335
                     /locus_tag="PKL17_12090"
     CDS             13412..14335
                     /locus_tag="PKL17_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNK45519.1"
                     /translation="MKILVTGATGFVGKRLLRRLLLRGDKVVALTRSRRNLSTQDASD
                     RIVVVEGDPAKPGDWQQQLAGCDAIVTLAGEPLFGERWGTSFKDRLIDSRVESARQIC
                     ASLAQLGPESRPRVWVAASAIGYYGARGDEGLTEQSSPGDDFLARLCMRWEEETRVAE
                     SLGVRVVLLRIGIVLGDGGGMMSKLVPAFRMFAGGPLGSGRQFVSWIHLDDVIGLLEL
                     ALERSSLSGPLNVTAPTPVRMSELARTLGEVMHRPSKMAVPELALRIAVGEAADVILA
                     SQRVLPDRALSVGYNYKYPELRAALQSIVSS"
     gene            14459..16003
                     /locus_tag="PKL17_12095"
     CDS             14459..16003
                     /locus_tag="PKL17_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNK45520.1"
                     /translation="MLAKTLSAGVLGIEAYPVHIEVDTSLGLPQYNLVGLADSAVKEG
                     AVRVRAALENSGFALPPRRVTINLAPADVRKDGAAFDLPIALALLVALELLPEKSLDG
                     KLWLGELGLDGSIRRISGCLPVALRAQKEGISELVLPADCATEAAAIRDLTVYAASSL
                     RQVVEMLQGGAPLPRAAERPLASSVGEPSCDLSDVRGNEQPKRALMIAAAGGHNLLML
                     GPPGTGKSMLARRLVSILPPMSESEALTTTAIYSAAGLLSGQSLLQQRPFRAPHHEVS
                     VVGLVGGGSNPRPGEISLAHHGVLFLDELPEFPRAALESLRQPLEERQITIVRARCSV
                     TYPASFSLIAAMNPCPCGFRGSSLRTCRCDLGVVSRYLSRLSGPLLDRFDLHIEVPHV
                     EYQTLLDARRSGVSSASVRDQVMAARAMQHERVETFGPFAHSNATLSPDAVVDVGKPD
                     AAGEKLLLAYAKKHVLSNRAVHRVLRVARTVADLSQERSVGAAHIAEALSLRILDRPG
                     SGIGSA"
     gene            16077..>16979
                     /gene="recA"
                     /locus_tag="PKL17_12100"
     CDS             16077..>16979
                     /gene="recA"
                     /locus_tag="PKL17_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNK45521.1"
                     /translation="MSVSKAVKEKLKAIATAVASIEKQFGKGAIMALDSREVADVQTV
                     PSGSIGLDAALGVMGYPRGRVIEIYGPESSGKTTLTLHAIVEVQKQGGVCAFIDAEHA
                     LDRSYAAKLGIKLDELLIAQPDCGEQGLEIADSLVRSGGVDLIVIDSVAALVPKAEID
                     GEMGDAHLGLQARLMSQALRKLTAASAKANCTVIFINQLRQKIGVAFGNPETTTGGNA
                     LKFYASVRLDIRRIGQIKQGESVVGSRVRVKVVKNKLAPPFRDAEFELLYGVGVNRAG
                     ELLDLAASHGLIEKSGAWYSLEGER"
BASE COUNT         3048 a         5001 c         5442 g         3488 t
ORIGIN      
        1 cccccggtct acaccgccgg gaccagcgcc gacagccaga gcgtgaggcc cgctgcccac
       61 agtcctgtgt tgcgcttttc gaccgtgtgc ttttgcatag accatggcac cgccgctgcg
      121 ggatgcggat agcgacgaat ttgcgacatg accagggtga tgtccgcctc ttgcatgcca
      181 ctgctgcgga gcgcgttcgg cagcagttct tcgtagcagc gctgcgccga atcggtccgg
      241 ccttctcgct gctgacgcag gcagtagtca tacaccgcgg tgaggcttgc tcccgacggc
      301 gtggtggtca tcgacgatgc aagccccggt ggtacggcgg gtggcggcgg ggcgggcggt
      361 gaaatcaccg gctttggcgg tggcgtggtg gcaagcagtg gacgcggtgc tggcgtcggt
      421 ggatcgtctt cttcgacaat caggggcggt ttgcgattgg tggcggcggc aggcgcggtg
      481 ggggcgggcg tcgggcgggc actcgacgaa gccggaaaga gcccactttt ggcatcggtc
      541 acacactgct cggcaaagcc tcggtacatc ggttctggac gatctgccgt cagcgacagg
      601 tacgactgac aggcatccat cgcttccgct ttccgaccga gctttagctg cgtctggcct
      661 atccgcacca gcatttcggg cgtgtttgtc gcgtcgtagg cttccttgaa cagacgcagc
      721 gcttcttcgt agcgtgcttc ggtgaacgcg cggagcgcat cctgatgcag ttcactcgga
      781 tctttggccc acgccgtttc gctgacaccc agaccgaaaa acaagcccag cgcgcacatc
      841 catcgtcgat tccgcattgc aaaggccctc tctcgatcca aggattggcc cgacgctgcg
      901 caaaacgcaa gttccttgcc gaaacgccga cggaagattg cagatcagag ccgacgcagc
      961 gaaagcgtca ctgtgccttc ggtgactcgc tcgccgtttg tgtcagtgac atcgacgagc
     1021 accgcgacat cgaccttgcc ttcggcgacg agccgctgtt tcagctcgct cgggtcgctt
     1081 acgccaagct gagcactggc ccacaggtcg ccgcgggccg gcttgcgaaa gcgcagctcg
     1141 cagcccttgc tgtggcaggt cacgtccatt ccgttcagga acaaaagccc agccgaaagc
     1201 gctgcggttt cggcggcaac aaactgcgca ccgacgtgaa cggtgccgcc atggttgcca
     1261 agctgggaat caaacggcag caaaatccgc gctgcggagg gggaaagatc gaccgcttgt
     1321 agcccgagct tttggaccat tggaattttg gcggtcacaa gctcgaacac atccgcagga
     1381 ttcatgcaaa agcccaggtc agcttggcgt tgtttggttt gttgtcgcac aggaccgtgc
     1441 gagcagaaag gggacgctag accccagcta cggggacgac tggggtttgg tatggctaga
     1501 tgccactcgc gaaccggagt cgccagacca tgcccatggc ctcggcgaaa attttgctcg
     1561 acatcttgga ctgaccggcg cggcgatcga tgaaaatgat cggcacttca gcgacggaat
     1621 aacctttgcg cacggcgcgg taggtcattt cgatttggaa gctatagccg ttggaacgga
     1681 ccgcatcgag gtcgatcgct tcgagcactt cgcgacggaa gcacttgaat cccgaggtca
     1741 gatcgcgaac cccaagcccc aagatcgtcc gcgcgtagag gctgccaccg gcggaaataa
     1801 gctggcggct taggccccag ttttcggtgc caccgccggg aactcgacgc gatccgatga
     1861 ccacgccggc accgtctttg gccgcagcga gcagcgccgg cagatacacc gggtcgtggc
     1921 taaagtcagc atccatctcg aagatgtact ggtagtgctt cgacagcgcg taacggaaac
     1981 cggcgatgta ggcggtgccg agtccgagct tgccgctgcg gtggagcaca tgtatccgcg
     2041 gattggcttt cgccatttcg tcggcgagct gccccgtacc gtcgggagaa ttgtcgtcaa
     2101 cgatcagaag atccgccgtc ggaagtgccg ccagaacctg atcgcacagc gttggcagat
     2161 tgtctcgctc gttgtaagtc gggataatga ttaaggcttc attcatgcgc gcactttaca
     2221 ccacctgcgg attccggtgc aggggatgcg gcgtagcgag tggcctgttt tcgtcgtcga
     2281 tgcggggcgg acgcgaaaaa tgcgttgaca gaacggggca aaatcctatt caatccgacc
     2341 tgttcagcgc ttttcccgtg tagctcagcc ggtagagcag gtggctgtta accaccgggt
     2401 cgggggttcg agtccctccg cgggagcaag tgacacaggg ctatcaggaa actggtagcc
     2461 cttttttgtt ttgcgcgtcg ctcacaacct gctgcgtggc gtcaaagaac gcgcgacaaa
     2521 acgcacgatc gtgggcgacg ggattcatcg ccgcaggtcg tcgggcggcg aaatactggc
     2581 cgctgatgtg ctcgtaggcg ggatcgagcg ccagcatcac tgacgtcgct gctccgtcgg
     2641 aaactgagtc gggcccgtcc atgccgaatc ctgcctggag aagcttcgtc gagaccacgc
     2701 ccggatggag tgcattgacg gccactttgc cgcgcagacg ctcggccagt tcgacggcaa
     2761 acagcacatt cgcgagcttt gacgccgcgt acatctcata accgtcgaag cggcgcttgc
     2821 gcagcgtgat gtcgtcgagt ctgatgtggc ctcggttgtg ggcgatcgag ctgacgctga
     2881 tgattcgccg aagctggcag cgtggatcgg ccagcagcgc catcgtcagc gcgaagtgac
     2941 cgaagtgatt gaccgccatg gtcagctcga tgccgtcttc ggaaaccgct ggtgttttgc
     3001 aaaatacccc ggcgttgttg acaaggacgc gcggaaaagt cgccgcggta gcaagctcgg
     3061 cggccagctt ggtggctcca gccagcgacg ataaatcacc gatgacaggt gcgggctggg
     3121 ggcggcccga ggcggcttcg acggcttttt gagcggcgcg gacgcgatcg gcactgcgtc
     3181 cgtgaacgat gacatcggcg ccgtgacggg cgagctgttt ggcggtttcc aggccgatgc
     3241 cgtcggaact gccagtgacc aaaatcgaag gacgcacgct gatcatggct ttttccaaaa
     3301 ctccttgcag acccaatggt aaccagatgc cgtcgcaaaa gaagtgtcag caggttgttt
     3361 gacttctgtt cctgtccacg tcttagccat cggttttttt cgattggttt cggcgaaaaa
     3421 tggccgatgc tcaaaatgga tgaccgggcg caaactgggc gcaggacgca aggagcagac
     3481 cgcatgatca cgatccgtcg cggtgtcgag cgcggccata aaaaccacgg ctggctcgac
     3541 acattccata cgttttcgtt ttccagctac tacgacccgg cgcacatggg ctttcgcaac
     3601 ctgcgcgtca tcaacgaaga tcgcgtcgct ccggggcagg gctttggcac gcatccgcat
     3661 aacgacatgg aaatcatttc gtatgtggtc gaggggacgc tccagcatcg ggactcgatg
     3721 ggcaacggat cgctgattcg accgcacgat gtgcagagga tgagcgctgg caccggcgtg
     3781 acgcacagcg agttcaatgg ctcgcagacg gagccggttc actttctgca aatctggctt
     3841 ctgccgtcgc agcgcggaat caagccgggt tacgagcaaa agacgttttc tcggcaagaa
     3901 aagcagggac ggctgcggct ggtcgcgtca accgatggca gagacggcag tgtccagatt
     3961 cacaccgacg ctgcgctgta cgcgacggtg ctttcggcgg gacaaacggt cgaacatgct
     4021 ttttcatcgg gacgccatgg ctacatgcag atcgtgcgtg gtgcggtgct cgcaaacggc
     4081 cagatgctcg gcgcgggcga cggtgcggcc ctttctggcg agcagtccgt ggtcatcgaa
     4141 ggcaaagatg acagcgaagt gctgctgttt gatctgccgt agcgggcgtc tcgtcgcgaa
     4201 gattaggcgg attcaatgtc gcccatcacc gcgaccacac cgcagactcc gacgacggca
     4261 ccatctcgac gatacggggc gagcgtaagc cacccaccgc gcgcttcccc gacgcgattt
     4321 tcaatcagca gcgcgtgtcg actcggcctt tgtttggtca ggacgtgccg gacctcgcgt
     4381 tcgatgcgtg gacaccactg gccgattttc gtcgtcgaca gcttgccgcg cagctcctcg
     4441 ggcgggaccc cgagcaaaag ccccagcgcg aagttgccaa acagaagttc acacgtcggc
     4501 gacgcgacaa aaagcggcag cggacaagca tcgaccagcg cttcgcacag atcggcgcgc
     4561 ggtgacaccg gcttggccgt atcgtccatt cggcggcgca tcccgaggct gttcatgatt
     4621 tgccgcagct caaaggcaaa gtcggcggcc tgctggtagc gctgacctgg gtctttggcg
     4681 agtgcgcgca tcaccaggtc ttcgagcgac ttgctcagcg gtggctgcgt ctctaccagc
     4741 cggcccggcg gaatcggcgt ttcgcgcagg tgtccataca aaatgtcctg aagtcgaccg
     4801 ccaaacgggg gtcgtcccgt cagacattcg taaaaaagca ccccgagcgc atacacatcc
     4861 gaggctggcg tcggcgtctg cacgcgaatt cgttccggtg ccatgtaggt cggcgttccg
     4921 gccactgtcg tcgctgcgac cagttctcgc ggatcgacat cgggacggct ggccagccca
     4981 aaatcgagca gccgcacatc attgcgccga tggtcatccc ccgtcgctga agcacacaaa
     5041 aatatgtttt ccggcttgat gtcacagtgg atcacgccgc gaccgtgcat gtagttgagt
     5101 ccctcggcgc actggagcat gatgtcgcag gctgctttcg tcgagagaac tcgctcgcga
     5161 ttgagtcgtt cggccagcgt ttcgccgtac aggtagtcca tgacgatgaa cgcgccatgg
     5221 tgggcgtcga cgccaaagtc gaacacgccg atgatgttgg ggtggctcat cgccgcggcc
     5281 atccgggctt cgcgataaaa agcgtcgcgg atgtggggct gtttggccag gtcatcgtgg
     5341 atgatcttca gcgcgaacag cttgtcgagg tcgcggtgac ggaccttgaa aattcggccc
     5401 atcccgcctt cgccgagaga gccttcgatc aggtagcgcc cggcgatgag ctgctcgggg
     5461 gagatgtggt tggggacgct gcttggctcg gccccgtaga tttcaaacgg gacattgcgc
     5521 tcgtcggtgt ccgccggcag ttgcagcgtt tcctgactgg ctttgggtcc accaaaagcc
     5581 ccccccggct tgtcttggct cacgggcgca ttatatcgcg gccttggccc attgctcgtg
     5641 ccttcgacgc tgtttgtctc tgcgccgacc acatgggggc tggggctttg aatgtgcgat
     5701 ttcgcacgaa tttgcgagaa cgcacgatgc ttgctcggca gcagacaagt tatgtacgtt
     5761 ccgccactat cgtgtccgac gaaactccgt ctgagaaaaa cccaccgcgg ggaatgctaa
     5821 ccaagctgct cggcgcgccg tggctgctgc tgttcttgac gttggccgtc ggtgcggtcg
     5881 gtttgtttgg cgtaagcaag ctcaagagcg aagaagactt gttggtcttt cttcccgagg
     5941 gagacgccga tgtcaccgtg tttcgcgatg tcgcatcacg ctttggcgcg ctgcgggtgg
     6001 cgctgattgg cgtcgagcca aagccggggc agaagctgtt ttcccccgat atgctatcgc
     6061 gcattgatcg cttgtcgacg cagctaaaga acaccaccgg cgtggatcgt gtggtgtctc
     6121 cgacgacgat aaatgatctg gtcccgatag aaggcggcgt cgatttgacg ctgcttgtgc
     6181 cacagccgat accgacggac caagcgacgc tcgacaaggt aaaagcgcgt gcgctcagcc
     6241 agccgacaat tcgtggcaat gtggtgtcgg cagacggtga agccgggctc atcatggtgt
     6301 ttgtcggcga tggcgtaccg acccgtcagc ttgccgaaaa agcccaagcc ctggcccgca
     6361 gcgagctttc gcccgtcgca agcttgtatt ttggcggcgc accgtttgcc gggcaggcga
     6421 tctacgacga cacccagcgc gacgtgcaac gactggttcc gatcgcgctt ctgctgtttc
     6481 tgtttgttgt tttgtcggcg tttcgggact ttttgtcggt gctgctcacc gtcggaacgg
     6541 tcgggctgtc ggtgctgctt gcgctagggc ttttgggctt gcacggcgac aagttcacgg
     6601 tggtgatggg gacgctgccg ctcgtgctgt ttgcgtcggg gagccagtac gcgatccaca
     6661 tccttgggcg ctactacttg attcgatcgg cggagccgtc gcgaaatgcg ctgtccgctg
     6721 ctcgtgaagc gctcaccgtc gcgggcccgc cggttgttgt cgcggcgctc aactgctgcg
     6781 tcggttttct gtcgtttttg gtgatgaaca tctcggcgat gcgcgcgttt ggcgtcgctt
     6841 gctgtgtcgg gatcatcgcc tgttgtgcgt tttcgctgac ggtggcgccg tcgatggtgg
     6901 cgcattttca ggccgatcgg ccagccaccg aggctagctt tgcccgtctt ggatcttttc
     6961 tgtccgggct gttttcatgg gtccgccgtc gacgggctgc gattgcgatt ggggtcgtgg
     7021 cactctcgtg tgtgtgtggc tatttcatga cgcgcgtgac ggttcggatg gaacccaaag
     7081 cgtttttccg tcctggttcg gaaccggcgc tggcgcagaa gtttctcgac gacaagtttg
     7141 gcggtgcagc gtttgtgcaa gtgctgctcg acggtgatat gaccgatccg cgcagtctgc
     7201 acgaagtccg gcggctgtcg gcgtttgcgc ggtcgctgcc gggcgtgacg caggtgcagc
     7261 acattgtcca gccgctcgcg atggtgggtg attcgatggc gggcctgcgc gggctgccac
     7321 aaaattcgca gcaagtcgtc gcgctgctgt ttttcctgga aggcgaaccg tcgctgcgca
     7381 ccatgctttc gcccgatcgc aagtcggtgc tggtgcatgt ccgggtgctc ggtgatgcgg
     7441 cgcaggtggt gagcgagctt cgtcgctatg tctcaagccg tctgccgcgc tcgccccagc
     7501 gtcaaagcct ggccgagctg tccgaagagc tgtcgtggct gttgcccccc gcggtgcgaa
     7561 acgagcgcac cctagcgatt tcacagacgg tggcggagct ggaaaaaaac gggcttgcac
     7621 ttgcgggccc agcgaagacg gacaagccag aaagtccgcc agccggcgac gtgaatgccg
     7681 atggtgaaga acaggcgcgg atgtcgcggg cccaggcgga agcaaagcgg cgactgctgg
     7741 cggttctggg ggccgaaaaa cccgacgcgc agggtcagcg actgggacgg cctgccgacg
     7801 atgtcgaagc gtgggtcgag tgggtggccg cggcgatctt gcagccgccc gtcgatgcgc
     7861 cgagcacaac actccaggct cgtttgaccg gtgagccgat gctcgaccaa gcattttcgc
     7921 gtgccgtgga tcgcaaccag tgggccagct tggcgatcgc gctcggcggt gtgctgattg
     7981 tgctgctgtt tgcgcagcgg tcgctcgttg gcgcgatttt gtcgacgctg cctgcggtga
     8041 tggcgctcgg cgtggtgttt ggaatccttg ggcttctggg caagccgatc gacttgggca
     8101 cgtcgctggt cggttccatg gtgacaagct ctggcgcgga ttttgcgatg cactacatct
     8161 ggtatctgcg ccggcagcct gcgagtcagg tggtcccgcg ggttgggccc gtgattttga
     8221 cgacagcgat cgtgcttggc ttggcgatgg gggtcatgat gctgggggcg tcaccgccgc
     8281 ttcgtctgtt tggcgcgctc gctgcggcgg gaatgctgct gtcggcgacg tttacgttcc
     8341 tgctcgtacc ggcgctcatc gggaagcttc ccgaagaacc aacgtcttaa cagttcgcac
     8401 gcgatgtttc gcaaggagaa ccgatgaagt ccacattcac caaactgctc gccttgggaa
     8461 cgctcagtct gggccttttc gcggcgccga cgactttcgc gcaaacgccc gcggcaccgt
     8521 ccggatcggc gccggtggtg caggaagaaa ccgcggaatc actgctgtcg cggatcgaca
     8581 agcagaacgc cgcgttttcc gacgcatttt tccatttcaa gatggtggtc aaagccgcaa
     8641 acggcagcgt cgagcgcgag gtcgaattta cgaccaagca aaaaggcaac cagaagcgac
     8701 tggtgcgttt tttgtcgccg ggcgacatca agggaatggg ctttctcctc gaaaaaccgg
     8761 gtgagatgta cgccaagctt ccggccttcg gcaatcgcat ccgacggctc ggaaccagcc
     8821 agatgaacca gagcttcatg ggatcggatc tgacgtcgga agacatgagc gcgatcgact
     8881 acgcgccgtc gtatgcgccg accaacaaat ccgctgacgg ggcactgacg gtgctggatt
     8941 tgcagcttcg cgccggcaag tcgtcggaat ttcctcgtct gaaaatgtgg gtcgattcgg
     9001 ccaaggccac gatcaccaag atcgagtact acgacaacgc tggcaagaag ctgcgcacct
     9061 gtgagcgctc agacttcaag caggacggcg aagcgcacta ctcgccgggc aagatgacgt
     9121 acatcgacca tcggcgcggc gaccacaaga ccgacatgat cctcgtcaag agcacgctca
     9181 acaacggcta caaggacgac gagtttaccc aacgcgcact tcagcaggac taaatgcgat
     9241 ccggccttct ttccctcgtt cgcgggctgc cgctagtttt ggcactgcct ctgtccgtgc
     9301 agccggcgcg ggctgatgtg gatctcaagt tttccggctc gatctcgtcg gacatgcgct
     9361 ttcgtatcca aggccaggat cgagcgctgc caaccccgac accgtcgcag tggacgcttt
     9421 tgaagaatgg tttttcgcgc aacgaaaacc gcatccgggc ccagcttggg gtaaagctgg
     9481 gacagaaagt tcgcggagtt gccgacgctg agctgatgct gtacgggttt tccgacctgc
     9541 gcgacattga ctctgcgacg ctgcgcgatc gggtcgatcc ctactatctg gaagtccacg
     9601 cggcgtacat cgacgtctac aacctgtttg gcgacgtgat accgcgcttc gatctgcggc
     9661 ttggccgtca gacggtgccg tggggagccg ccgacaagtt caacccgatc tcgaacctca
     9721 acacgcttga tttgtcggac ccgcttttgt ttggtcgcgc gcttggcaac aacatggtcc
     9781 gcgccgactg gaatcccgtc ggggatctgc aatttactgc ggtgctggtg ccgattttcc
     9841 gcccggcgca gctgccgcgc acggcgccgt ttgcgctgct ggatccgctg cgaccagcgc
     9901 cggtgctgga ccaaggcgtg cgaaccgcgc tcgacaacga acgggctgtc tatgtgccgg
     9961 tgcgcgatcc ggtcacgggc gtgcttctgc cggcggggcg aacgctgacc gtccaggccg
    10021 acgtgttggc gccggaagtg tcggcggcga acatgcagtt tggtgggcgc atggcctgga
    10081 aactgctcgg tcaagacatg gggctgtcgt actaccacgg gcgcttcgga attccggtgc
    10141 cggcgtggtc ggtgtcgacg ggaacgcttg ataacaacgt gacgctcacg cagcttgtct
    10201 atccgcgtgt cgacgtcgca gggcttgaaa tggcgggctc gatcgagcgg ctcggcggca
    10261 tgggctactg gatcgaagcg gcggtgaact tccccggcga agtcgaaatg ggcctttacg
    10321 acgcgatcac cagtccgaat gcgccgcggg cgatggaata tctgcctggt ccagacggac
    10381 gttactaccg caacacgctt cccgtcgggg ctcgcggggc gcgtccactg gtgattgata
    10441 agacgccgtt tcccaaagcg accgtcggtg ccgactatgc gtttggtggg cacgtctacg
    10501 tgaacatcca gtatgtccac ggcttcatcg acgagtttgg agccggtcgc gtcgccaggc
    10561 cacgcaaaga cgcgatcgat gtcagtgccc agccgcgggt cgaatcgcga atcggtgact
    10621 atgtggtcgc tggtctcgac gtgaagcttc tcaacgaacg gttgctgatg cggctgtttg
    10681 gtgtgctcaa gctgccgtcg attgatctgc aaagcaagct gtgggacgac tgggccccga
    10741 cgggcgtgct gtttccgcag atcatctgga acgtctttga cggcaccgag ctgatggtgg
    10801 gttcgtttgt gatgctcggc gatcgctcga ccaagtttgg cgatccggct gccggtgcca
    10861 ccgaagtctt tgtgcgcggc aaagtcagct tctaaccgcc gctgctctcg tctctcatct
    10921 tttcgtcgcc ctcgtccggc gctgcgcagc gatatactgt ccgcggaggt ttgtgccatg
    10981 gcaaagatga gcgtgaggcg gaagcttgcg atcgcctctt actcgccgcc gcgcgagggc
    11041 aacatttacg gcaagctggt cgtcgacgcc acacccggct tgggctacct cgaagacgtt
    11101 cgcaaacgga cgggcgaaaa ggtgtccttc acccatttgg tcgggcgagc ggtcgccgaa
    11161 gcgctgcggc aagccccgac gctcaatggc cgcatcgtgt ttggcgagtt cgagccgttt
    11221 cccaccgtcg acattgcttt tctcgtggcg cttgaagacg gccaagacct ggcccgcgcc
    11281 aaggtctgtg acgtcgacaa aaaatccatc gccgagattg cgcaagagct cagccgcaaa
    11341 tccggcaagc tgcgcgcgtc tgccgatccg gcctacgaaa aatccaagca agcgatcaag
    11401 atgatgccga cgtggttgct tcggcccatg ctcgacgtga ccggctatct ggcgagcagc
    11461 ttgggcctga gcattccggc gctcggcgtc gagccgtttc ccttcggatc gtgcatcgtc
    11521 accagcgtcg gcatgttcgg tttggaagaa ggctttgtgc cgccgacgcc gtttacgcgc
    11581 gtgccggttt acattctcgt cggtgcagtg aaagagcgtc ccgcggtggt cgacggaaaa
    11641 gtcacaatcc agccgcagct taccctgacc gcgaccattg atcatcgctt catcgacgga
    11701 gcgcagctcg cggtgctggc caaagtggtg cgcagcatcc tcgaaaatcc ctggcagctc
    11761 gatcgcaaag acaaaagctg acgtccggcg cgggcttccg ggctacacgt cgaccggctg
    11821 cccgtttttt cgccgctcgc gctgggccaa aagccagctg cgtgcttcgg cttccccgtc
    11881 gaacatgcgg agctggagcc gtcggcccga tacgagtcga atcgcattga gcaagagcat
    11941 cgccaccgcc gatgctgttc gactcgcacc aaacaccgca aaaatcgtct ttcccggatg
    12001 agcgcgcacc cagtctgaaa tgatccgccg gctttcggca tcggctgggg ttccgtcgcg
    12061 ggcatcaagc aggacatacg ccagaccgtg ggctgcgatg acgcgcgcaa ggatttcgtt
    12121 tagcagcttg ctgatgtggg gcggactcat atggccgtgt ggttcgacca caaccaggtc
    12181 gtcggtcacg agcgtgcggt gtccagcgta ggtcaaccaa tcgcccatca tgacaaagat
    12241 atcacgcagc ggcccaagct cactcgtcgg cagtgactgc gattttggcc gcgccgcggg
    12301 cgtgtgatac tcagcatagg gatgaaccga cgacggtttt ttcagctttc ggcgctcggg
    12361 acgataggtc ttggggcggc gctttgggtc ggcgagcaca cgctgggtcg tggcgtacct
    12421 gcgaagctcg accagcacat ggaaggaacc gtgttgtcat cgtcagaatc gcagacactt
    12481 cgagcggttt cgctgcgaat tctcgatggc gcagttcctg acccagcggg cgatggcggc
    12541 gctgcgatct gtcgttttgt cgatcgctac ctgctggaat tgccgcgtcc gcttcagcgc
    12601 gatgtccgtg cgctgctcca cctggtcgag ctgtttccgc tgctgtcgct tcgcatggcg
    12661 cgtttttccc atctccgcgg cgatgagcaa gacgcgatgc tggcggactg gcaggacagc
    12721 cgcattggac ttttccgcca aggtttccaa gcgctcaagt cgctgtgctg cctggcccat
    12781 tatcaggacg cgcggagctt tgccgcgatt ggctattccg gtccgcttgt gtgatggcga
    12841 tgctggcacg cggtctgctt tgcgcctgtt tcgatgcgcg caaaactgct cccgagctac
    12901 tggctgctcc tatcggtgtt tgtttgcttt gctggatttg tcggttttcg ccgacccaag
    12961 tcgctgtcgc tgcgtccgct gccgctctgt ccatgtgaca ttcccgtggc acttccagcc
    13021 gggatgattc gctgtttgtc gacccaagag gcgctggact ggaatgtcca ggcgggcgat
    13081 gtgccgccac tgtccgcttc gggaacacgg ctgtttggac cgccgaatcg aatgtcgggc
    13141 gaacggcagc tcgcgctcgg tcttccgatc gacccaaatg aggcgagcca agccgacttg
    13201 gaggcgcttc cggatgtcgg tccggcactc gcgcaggcga tcgttaccgc gcggcgacag
    13261 cgtcgtttcc tgaccgaagt cgaccttctg gccgtgcgtg gacttggacc gaagcgactg
    13321 gccaagctgc gtccttatct acagcttcgc aaaacgcggt aggctgacgc ggcccggcat
    13381 ctaatccggc aaacagcagc aaggagcagc gatgaagata ctggtgaccg gcgcgacggg
    13441 atttgtcggc aagcggctgc ttcggaggct gcttttgcgc ggtgacaaag tggtcgcgct
    13501 gacccgcagt cggcgcaatt tgtcgacgca agacgcatcc gatcgcatcg tggtggtcga
    13561 aggcgatccg gcaaagcctg gcgactggca gcagcagctc gcaggctgcg acgcgatcgt
    13621 gaccctcgca ggggagccgc tttttggcga gcgctggggc acttcgttca aagaccgtct
    13681 catcgacagc cgagtggaga gtgcccggca aatctgcgcc tcgcttgcac aactgggccc
    13741 cgagtccagg ccgcgcgttt gggtcgccgc ttccgcgatt ggctactacg gtgcgcgtgg
    13801 cgacgagggg ctgaccgaac agtccagtcc cggcgatgat tttttggcgc ggctctgtat
    13861 gcgctgggaa gaagaaaccc gcgtcgccga gtcgctgggc gtgcgggttg tgctgcttcg
    13921 cattggcatc gtgcttggcg acggtggcgg catgatgtcc aagctggttc cggcgttccg
    13981 aatgttcgcg ggggggccgc ttggcagtgg tcggcagttt gtctcgtgga tccacctgga
    14041 cgatgtgatc ggacttttgg aattggcgct tgagcgatcg tcgctgagtg gcccgctcaa
    14101 cgtgaccgca ccgacccccg ttcgcatgag cgaactggcc agaacactcg gggaagtgat
    14161 gcatcgtccg tcgaaaatgg ccgtaccgga actggcgctg cgaatcgccg tcggggaagc
    14221 tgcggatgtc atcttggcca gtcagcgcgt gctgcccgat cgcgcacttt ccgtcggata
    14281 caactacaaa tatcctgagc ttcgcgctgc cctgcaatcc atcgtttcct cataagcgca
    14341 aacggcccca aaatcggaca acgtccggta taccggacac gggcggcttc gcgtttctct
    14401 gctctcgacg ggaaaaatgc cagcgcgctt ggcatgatgg ctgctttggc tccgtgctgt
    14461 gcttgccaaa acgctgtccg ctggggttct cggtatcgag gcctatcccg tccacatcga
    14521 agtcgacaca agtcttgggc tgccccaata caacttggtc gggctcgccg atagtgccgt
    14581 caaagagggg gctgtccggg ttcgcgccgc gctggaaaac tcgggttttg cgctgccgcc
    14641 gcgtcgggtg acgataaatc ttgcgccggc agatgttcgc aaagacggtg cggcgttcga
    14701 tctgccgatt gcgctggcgc tgctcgtcgc gctcgaactt ttgcctgaaa agtcgctgga
    14761 cggaaaactg tggcttggtg agcttggtct cgacgggagc attcggcgga tcagcgggtg
    14821 tctgccggtc gctctgcgcg cccagaaaga gggcatttcc gagctggtgt taccggcgga
    14881 ctgtgcaacc gaggccgcag ccattcgcga tctgaccgtg tatgcggcga gcagtcttcg
    14941 tcaggtcgtc gagatgctcc aaggcggggc accactgcca cgagctgccg aaagacccct
    15001 ggcatcgtcc gtcggtgagc caagctgcga cctcagcgat gtccgcggca atgagcaacc
    15061 caagcgggca ctgatgatcg ctgcggcggg cggtcacaac ctgctgatgc taggtccgcc
    15121 ggggacgggc aagagcatgc tggcgcgacg gctggtcagc attttgccgc cgatgtccga
    15181 gagcgaagcg ctgacgacca ctgcgatcta ttccgccgct gggcttctct ctggccagtc
    15241 gctgctccag cagcgtccat ttcgcgcccc gcatcatgaa gtgtcggttg tcggtctcgt
    15301 cggtgggggc agcaatccac ggcctggaga gataagcctt gcgcatcacg gagtgctgtt
    15361 tctcgacgaa cttcccgagt tcccgcgggc cgcgctcgag tctctgcgtc agcctctaga
    15421 ggaacgtcag atcaccatcg tgcgggcgcg ctgctcggtg acatatccag cgtcgttttc
    15481 gctgattgcg gcgatgaatc catgtccgtg tggtttccgt ggctcgtcgc tgcgaacctg
    15541 tcgctgcgat ctaggtgtgg tgagccgata tctttcgcgg ctgtcgggcc cactgctgga
    15601 tcgcttcgat cttcacatcg aagttcctca cgtcgagtat cagacgctgc ttgatgcgcg
    15661 gcgtagcggt gtgtcgtctg catcggtgcg cgatcaagtg atggcggcgc gggcgatgca
    15721 gcatgagcgc gtcgagacgt ttggtccttt tgcccatagc aatgcgacgc tcagtcccga
    15781 cgccgtcgtc gatgtcggaa agcccgacgc tgcgggtgaa aagctgctgc tcgcgtatgc
    15841 caaaaaacat gtgctgtcga accgagccgt gcatcgggtg ctgcgcgtcg cgcggacggt
    15901 ggcagatctg tcgcaagagc gctccgtcgg tgccgctcac attgccgagg ctctttcgct
    15961 gcgcatcctc gaccggccag gcagcggcat tggctcggcc taggtgtttc tttcgtccag
    16021 gctcgctgtg cgcctggctg acacaaccgc aatccccacc cccacaagga gagaatatgt
    16081 ctgtctcgaa agccgtgaag gaaaagctca aagccatcgc aaccgccgtc gcctcgatcg
    16141 aaaaacagtt tggcaaaggt gcgatcatgg cgctcgacag tcgagaagtc gccgacgtcc
    16201 agaccgttcc atcgggctcg atcgggctcg atgctgcgct cggtgtgatg ggctacccgc
    16261 gtggccgcgt catcgaaatt tatgggcccg agtcgtctgg caagaccacg ctgacgctcc
    16321 acgccatcgt cgaggtacaa aaacaaggcg gtgtctgtgc attcatcgac gccgaacatg
    16381 cgctcgatcg cagctacgcc gccaagctcg gaatcaaact cgacgagctt ctgattgcgc
    16441 agcccgactg cggagaacaa ggcctggaga ttgccgactc gctggttcgc tccggtggcg
    16501 tcgatctcat tgtcattgac tcggtcgcag cgctggttcc caaggcggaa atcgacggag
    16561 aaatgggcga tgcgcacctt ggccttcagg cgcgactcat gtcgcaagcg ctgcggaaat
    16621 tgacggcggc ttcggccaag gccaactgca ccgtgatttt catcaaccag ttgcgacaga
    16681 agatcggcgt cgcgttcgga aaccccgaaa cgacgacggg aggaaacgcg ctgaagtttt
    16741 atgcctcggt gcgtctcgac atccgtcgga tcggtcagat caagcaaggt gagtcggtgg
    16801 tcggctcgcg ggtgcgcgtc aaggtcgtca aaaacaagct ggcgccgccg ttccgcgatg
    16861 cggaatttga gctgctctat ggcgtgggcg tgaatcgcgc gggggagctg ctcgacctgg
    16921 cggcgtcgca cggacttatc gaaaagagcg gagcgtggta ttcgctcgaa ggggagcga
//
LOCUS       DAORGN010000099        16967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1370_length_16967_cov_6.050024, whole genome shotgun sequence.
ACCESSION   DAORGN010000099 DAORGN010000000
VERSION     DAORGN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_16967_cov_6.050024"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1860)
                     /locus_tag="PKL17_12105"
     CDS             complement(85..1860)
                     /locus_tag="PKL17_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNK45522.1"
                     /translation="MTRSKGLRISSLVACGAAALLFAQAADPVANAAPVARPAAVAGR
                     PAVAAAPAAKPGAKPAVPAVSGPKLVVLIVADQFRADNLTRWGHLLSEGGLKRLMKDG
                     ARAVGRYGQQNTYTGPGHALIATGSYGYLNGITQNKFWNRQAGRAESMLYDGNVKILG
                     EKELSLEDDSSPRNLIGSTVFDELRFASKDSKVVSVSLKARGAILLGGHTGHAYWFSD
                     QSGEMTTSTFYTQDTAEWVKRWNSGKMADKLFGKVWDRLLPESAYTNIDASPHEADLK
                     GLGRVFPHPTNAKQQAPGPGYYESINYTPTGLEIQMDFARAAIDGEQLGQRGVTDAIA
                     ISVSGTDLAGHLFGPYSHEYQDMVLRFDRAVEKLLQDLEKKFKPGEFVVAFTADHGAA
                     PIPEEAMQKSLLAGRVRKGIVKDTVQKALSQFYSIQGEWVAALEDPSVYISPKMIAQA
                     KADPAQVEEVAGRAVLEIPGVIGYFTRTQLMRGWMPPTDVSTAVVRSYFPSRGGELVL
                     VMAPFYFWGKYGEKEQGTSHGSFYRYDTEVPVVFYGPQFNPGEYGVIDQVDFAATLAH
                     SLKLTPPAACAGRPMMPMLKPRTVP"
     gene            1975..5304
                     /locus_tag="PKL17_12110"
     CDS             1975..5304
                     /locus_tag="PKL17_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNK45523.1"
                     /translation="MSPLRFTLLSAAGLGQAVPLYELALSTAPDELRARRLGPVGQVL
                     RDFLLAWEASQRSDLRDGGGLCKAVGRLLLCAESVGASPKLLLRATQRLQEPTTTDEA
                     EPQRAAQAGRIAAAASLLLRLSETYGRRAASDARLFAEGLRKLALPPLVEAAADIELV
                     DLHLALVARDDGLGLPAWLAAIDCLAKTDRPLRIVLPSVDDRPPLQRALKPIFDSIYQ
                     RHELAIELSESGSLGPFAEGTTPWSRLIRSLFRPKSGGSLVSPLELADRVTLTPSAGP
                     QAEAHAVAARVQNLLRSGVAAADIAVVAESAARRRRLTAALHRAGVPTTPLSGGVAGP
                     QTRTAELPPPLRLICLLFEVLKLGLPREGFVQLLTSRYVRFPGPLGDKPWLVAQALRT
                     AGVRTLRHPERSRPSTGNLWESARSEPTWGLCRLRTWLATQQKPASDDKPRSRPPHLP
                     HLVEQVEQALHELTSLPSQATIADHCRALVRLLLRLHFFERCTTLPPLPEESEAESAT
                     LCMQLLDAHQRDSACVGVLSQCLDELPKWAARLGQATLDIGQREFAQLLRMTLGRLWN
                     EVAEAPQPHAVSIGELAEIAPRTFAHLFIAGLIDGELPTFRPEDSLLPDEDRRLLDRF
                     ADRPTWPLVSQQNDAEPLKLAVALAHVEHAHLFWPLADEEGRPLPHSQFLDEICFAAA
                     IPDDKIKLRPDPPATIHPTELWQRAPQHQSLFSALSRRERARANRLTSILRIEEQRSR
                     FFSATIADADAANLYSHPFVGRLLDDSLITVLQPRLPGSPKHPLSASVLEDYARCPFR
                     FFARRVLGIRPASEGSEELDPLASGRLHHAVLEHFFIERKESKRLPLRGDEADEAALD
                     AAIQRVVSDFEQTEHIGNPALLKVRLGRLRAELWRLVKNEAAKPPEPGCLPTLFEWKF
                     GPLSIASAQGDEGRMALHIHGIIDRVDIGGGKAVVLDYKAGRLDRYQAQLRDKLLKTS
                     FQLPLYVAALRADPSLRESTPLQSVVARYYSLRQGKVSNSLDDAEMTTLLPEVRLRNP
                     DGNVAEVAYRLWRRLRDGDFAVVPKTCEGCGLESVCRISSAPLDVPPEPSEETASSES
                     QENPAPSQLSPTRDFDEGDGR"
     gene            5301..>8704
                     /locus_tag="PKL17_12115"
     CDS             5301..>8704
                     /locus_tag="PKL17_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNK45524.1"
                     /translation="MSPPLRFLLLRDRVVQAGAGTGKTHALLSQYLHLCAGATAHGRP
                     IPPQQICALTFTEKAATEMRERLVRRVTAICRQLGAVGSEDAATALAETEPDLVASAA
                     HLGSKLPSIESWDQVLSSLSGATISTFHAFAAALLRRYAQVIGVDPEFALLDEDSAAQ
                     RLRETCEELVLSALEEPTTISSDPEFPSIVAQLLTELGFSSGIGGDGGLIEVLCRLHR
                     QLAEEGQPGDRLTSAYDQTNFVTALADARGLLLSSLQELAARADEIGGKRGEKAALLG
                     GLAAQIGQRLASIDDIAALRSEFGQVTDCFRTIRGPSGAKADPVLSQSIKDWKQQAKD
                     AIDDLQALSVSQKAAPLAKGLALLLGPLSERFSRLKHEEAVLDFSDLLSLARNLLRDH
                     PTVRHELRQRFQVFLIDEFQDTSPIQAELVTLLVGDEGHSPGRLYIVGDRKQSIYDFR
                     GADVAAFTRLCQRLRELGADEETLDRSYRSLPGVIQFVNRLFAQVMQPPSGQLPDWFV
                     RWDPENDPLKAQRSDETVATPQVELLRPTNPAPANPQGDAVKIPSIVREANLIAERID
                     ALLADGVRPGQIVVLLRRFTHLLHYTTALRRKRIPHYVVRGRGFFAAQEVRDLASLLR
                     LLDDPSDVQALVAVLRSPLCGLSDESLARLHLAGRLSLPALCETPSSPGEIPDEPDLD
                     DQPAHLLLRLPADEMNRLRHIVSLLGLLTTEADRFDPADLFTIAIEACDYRAVLAADP
                     DGGQRLANLDRLIERARAYSGRLRAFARFLRLQTDPDLARLNGDPSDEPAAQMLSEHD
                     DAVRIMTVHQAKGLEFPTVIVGGCTTRERHDTPQVVYDRELGLGLSIYVDGERRMTLQ
                     QKRIATRRKQRVRAESDRLFYVAATRAKDRLIWAGESDSGIGSTWRQPIEQLIGEADT
                     AALCTEWSRATPAPESAAEPPTKQVPTTDPAAAEESVRLFYEPPLPPSTKTTLVTALA
                     AERLACARRYQLLAQRPAPSSRPQQRARTSDETMHQTAQACLLAGTLAQRLLSVVDLS
                     RRGEDVSRAVAQLGILRDSALAAEAQTWLHTLLRSRWLSDALGQADCFSSLRRGLCYH
                     VPIATDSPLWLSGVLDLTWLGTDGTPHVVDIQLLTPPTKEHAFFVAR"
     assembly_gap    8705..8804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8857..9651)
                     /locus_tag="PKL17_12120"
     CDS             complement(8857..9651)
                     /locus_tag="PKL17_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45525.1"
                     /translation="MRVLFRSVLLTSVLLGACSSGPKYKIDDALLADVSMGEKSQMLQ
                     IQSDLNQATEEKNKAVSDASIADKELTVADAEYDKAKADVNMAKAERDLADSTKDLNR
                     MNAAKSNLEQKQLAKDVADSKIDYAKARRSHARSLVDAMEAKISALRSRYEQSKAQLA
                     AAKGKKPTADFSVATFDQQASDAQRKADDAKLTADRRQLEATQAQGKYNQLFQKLQMM
                     TGTQMNQGMPGMQPGMQQPMQPGQPGMQPMQPGQPGMQPMQPGMQQ"
     gene            complement(9763..10290)
                     /locus_tag="PKL17_12125"
     CDS             complement(9763..10290)
                     /locus_tag="PKL17_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45526.1"
                     /translation="MGKPVKKRRQLLALAIGLCSLSLPQIAWAQPDPSKANPPDQHAA
                     TKASCAVRVIHATKQGGQFDTRLEPLRPQLTRPPLDSWKGFALLRQHELVIASGSSQT
                     FEVPGDHQGVLSYEGRADAAGKLRLRLKLEIQDGSAKLLSTKFVINNGGTVLQAGIQH
                     DKGLLVLGFTCTASS"
     gene            complement(10275..10745)
                     /locus_tag="PKL17_12130"
     CDS             complement(10275..10745)
                     /locus_tag="PKL17_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45527.1"
                     /translation="MAVTDDKLHKLFDNELTSDEAEALRRELSDEELAKLAVLSALSQ
                     SVHDAVETEAAAHSIDVWSQIEGKLDDRPTSNVVPLRRKVLRRTTAVLSALALAATLL
                     FLLRPSASRDNSCDVEELEVVGGGATVLKVADEHGNDTTLIWFDHQEEDEWESL"
     gene            complement(10758..11450)
                     /locus_tag="PKL17_12135"
     CDS             complement(10758..11450)
                     /locus_tag="PKL17_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45528.1"
                     /translation="MAQTTDSSAKVIPPPTPVEPGKPSEATDEQRRAEAELVDRSRRG
                     DQKAFRILVERYQRRVFSLAFGLLKDPDEARDISQEAFLKAHRHLGSFQGTASFYTWL
                     YRITVNLCIDRKRRVGRGSEVELDERLSHRQVGSPADVLSAQKLSFNPQRVAQSSELR
                     QRILVALSQLSDQHRTVLILREVEGLSYKEIADSMDCPEGTVMSRLFHARRQMQELLS
                     DFAEDAEQEGAE"
     gene            11583..12137
                     /locus_tag="PKL17_12140"
     CDS             11583..12137
                     /locus_tag="PKL17_12140"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNK45529.1"
                     /translation="MPDSIILSCLLIFFARVGDVSLGTLRVVMVTQGRRGRAAMLGFF
                     EVLIWVFAASRVIGSLGKPQLAVAYAAGFACGNYVGLTLERWAAMGNQVVRVFSRRGE
                     QLADELRQHGFRVTLFHGLGRDGPVHMLYVETERRRIPFILNEARRLDPQCFYVVDDV
                     RIASSVDDPPRKRWLSAYSIRQRK"
     gene            complement(12165..12809)
                     /locus_tag="PKL17_12145"
     CDS             complement(12165..12809)
                     /locus_tag="PKL17_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNK45530.1"
                     /translation="MKKIAVLGSGTVGEVLANGFLKHGFSVMRGTRESAKLANWQTQA
                     GERASVGSFAEAAAFGDVVVLAVKGSVVEELVTSLGSVLDGKTVIDTTNPLAEAAPEH
                     GVLKFFTGPNDSLLERIQKAAPKAHFVKAFSCVGNAFMVNPDFGGQKPSMFICGNNAA
                     AKEQVSKILDLFGWEIEDMGHATAARAIEPLCMLWCIPGMIRGRWTHAFKLLHK"
     gene            complement(13012..13395)
                     /locus_tag="PKL17_12150"
     CDS             complement(13012..13395)
                     /locus_tag="PKL17_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNK45531.1"
                     /translation="MSISKSVGDFGEERAAIYLVEHGAEVLARNYRTRRGELDIVARD
                     AETLCFVEVRLREDDRFGAPSETVSVTKQRRLYYAAMEYLLKQCQHMNAVACRFDVIS
                     IERTARKITWIRNAFVWNGQSNRPM"
     gene            complement(13619..14029)
                     /gene="rplS"
                     /locus_tag="PKL17_12155"
     CDS             complement(13619..14029)
                     /gene="rplS"
                     /locus_tag="PKL17_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNK45532.1"
                     /translation="MSDVVPAVELLEKTLLRKQALPEFRVGDSVRVWVLIKEGDKERA
                     QAFEGVVIRRHHHGLRSSFTVRKISYGVGVERIFPVHSPRIDRVEVVSRGEVRRARLF
                     YLRDLSGKAARIKERTTKRTESAAAEPAPAAAEG"
     gene            complement(14042..14647)
                     /locus_tag="PKL17_12160"
     CDS             complement(14042..14647)
                     /locus_tag="PKL17_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNK45533.1"
                     /translation="MSVQATSGFATLAARSFVALVHHPVYDKQHQVVSTSVTNLDIHD
                     IARSCCTYGVAGYYVVHPVAAQRQLVERILGYWHEGPGQSQNDFRREALARVRVVQTT
                     DEAIDAITREHGQRPIVVGTTARPSVLAVSIPALRAQLLREARPLFLVMGTGWGLTDE
                     FMSKVDSVLEPIQGTAPYNHLSVRSATAIMLDRLFAEEAGT"
     gene            complement(14644..15408)
                     /gene="trmD"
                     /locus_tag="PKL17_12165"
     CDS             complement(14644..15408)
                     /gene="trmD"
                     /locus_tag="PKL17_12165"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNK45534.1"
                     /translation="MAQSIDIVSIFPEMFDPVLRVSITGRAIAKGALVVRAHNLRDHT
                     TDKHRTTDDTPYGGGSGMVMLLEPMVRALQSIEAAHGKARKIVLAPTGKPLTQKLVRE
                     LATEPRLIFMCGRYEGMDERIYHYVDDEISIGDFILTGGELPAMILVDALSRLIPGVL
                     HNEQSSQDESFECGLLEYPQYSRPAEVAGLPVPPVLLSGNHEHIRRWRRQLSLVRTRQ
                     RRPDLFGSVQLTDEDRRLLAAWDTEHSLRESSEAPT"
     gene            complement(15408..15947)
                     /gene="rimM"
                     /locus_tag="PKL17_12170"
     CDS             complement(15408..15947)
                     /gene="rimM"
                     /locus_tag="PKL17_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNK45535.1"
                     /translation="MSRTLLEIGAVCRPHGLRGELRVKLHDPASNALDMATHLWTEKE
                     AERPKQWQITSCRGESNGFFVVSVSGIADRTAAEALTRQKLLMERADLPELEPGEFYL
                     ADLPGCEVLTTDGERVGVVQEVMTVGAQPQLVIARPPRESALIPAIPEFLASFDADRR
                     QVVISPPAGLLDLDQPESL"
     gene            complement(16163..16432)
                     /gene="rpsP"
                     /locus_tag="PKL17_12175"
     CDS             complement(16163..16432)
                     /gene="rpsP"
                     /locus_tag="PKL17_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNK45536.1"
                     /translation="MAVKMRLARVGAKKAPFYRVVVANSRSPRDGRFIEHVGVYDPTR
                     TPAEIRFKMERVDYWLSQGAEPSDTVAFLISQVKTGKAAPAAASK"
     gene            complement(16665..>16967)
                     /locus_tag="PKL17_12180"
     CDS             complement(16665..>16967)
                     /locus_tag="PKL17_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45537.1"
                     /translation="DAEFELLYGVGVNRAGELLDLAASHGLIEKSGAWYSLEGERLAQ
                     GRERAALWLAEHPVVMESLRERLLAALRRGEVSPRSVTPAEDAATADEEASSKEAA"
BASE COUNT         3284 a         5410 c         4833 g         3340 t
ORIGIN      
        1 cactcgcgac gctgtacgcc aagggctaac caacatcacg atctcccaaa ccttgagcca
       61 aaagaaaacg atcgcccacg cccactaagg aacagtccgc ggcttgagca ttggcatcat
      121 gggccgaccg gcacaggccg ccggcggggt cagcttcagc gagtgggcca gcgtcgccgc
      181 aaagtccacc tgatcgatga caccgtactc gccgggatta aactgcggac catagaagac
      241 caccggcact tcggtgtcat agcgatagaa cgagccgtgc gacgtgccct gctctttttc
      301 gccgtacttg ccccaaaaat aaaacggcgc catcaccagc accagctcgc cgccgcgcga
      361 cgggaagtag ctgcgaacca ccgctgtcga gacatccgtc ggtggcatcc aaccgcgcat
      421 cagctgcgtg cgggtgaagt agccgatgac ccccggaatt tccagcacgg cacgcccggc
      481 cacttcttcg acttgcgccg gatcggcctt cgcctgcgcg atcattttgg gcgaaatgta
      541 gacgctggga tcttccagcg ccgcgaccca ctcgccttga atcgagtaaa actgcgacag
      601 tgccttttgg actgtgtcct tcacgatgcc tttgcgaacc cgacccgcga gcaggctttt
      661 ttgcatcgct tcttcgggaa tcggcgccgc accgtggtcg gcagtgaagg cgacgacaaa
      721 ctcaccgggc ttgaacttct tttccaggtc ttgcagaagc ttttcgaccg cgcgatcaaa
      781 gcgcaggacc atgtcctggt attcgtggct gtacgggcca aacaggtggc cggccagatc
      841 cgtaccgctc accgaaatcg cgatcgcatc ggtaacgcca cgctggccga gctgttcccc
      901 gtcgatcgcc gcccgagcaa agtccatttg gatctcaaga cccgtcggcg tgtagttgat
      961 cgattcgtag tagcccggtc ccggcgcttg ctgcttggcg ttcgtcggat gcggaaacac
     1021 acggcccaga cccttcagat ccgcttcgtg cggcgacgca tcaatgttgg tgtacgccga
     1081 ctcgggaagc agacggtccc agactttgcc aaacagcttg tcggccatct tgccgctgtt
     1141 ccagcgcttg acccactcgg cagtgtcttg ggtgtaaaac gtcgacgtgg tcatctcgcc
     1201 gctctggtcg gaaaaccagt aggcgtgccc agtgtgaccg cccagcagaa tcgcaccgcg
     1261 cgccttgagc gacacggaca cgactttgct gtctttgctg gcgaagcgca gctcgtcgaa
     1321 cacggtgctg ccaatcaggt tgcgcggcga gctgtcgtct tccaaggaca gctctttttc
     1381 accgaggatc ttgacgtttc cgtcgtaaag catcgactcg gcgcgaccgg cctgacggtt
     1441 ccagaacttg ttctgcgtga tcccgtttag gtagccgtaa gagcccgtcg caatcagcgc
     1501 atggcccggc ccggtatagg tgttttgctg gccataacga ccgacggcgc gcgcgccatc
     1561 tttcatcagg cgcttgagcc cgccttcgct cagcaaatga ccccacctgg tcaggttgtc
     1621 ggcccgaaat tggtccgcca cgatcaggac caccagcttc ggtccgctta ccgctgggac
     1681 cgcgggcttt gcaccaggct tggccgccgg tgccgccgca accgcaggac gtcctgcaac
     1741 cgccgccggc ctggcaaccg gagccgcatt ggcgaccgga tcggctgctt gggcgaacag
     1801 caaagccgcc gcaccacacg caacaagaga cgaaatccgc aaccccttgc ttcgggtcat
     1861 gttcaagctc tccagcacaa gaggccatcg tatcgtatgt gctgtcggtt tatctagaag
     1921 cgcttatttg ccgacggatg gctcgctggc tctggcatgt ggtatgcagc gtccgtgtcg
     1981 ccactgcgtt tcacgctcct atctgccgca gggttaggtc aagcggtccc gctctatgag
     2041 cttgcgctct cgaccgcacc agatgagctg cgcgcgcggc gactcgggcc tgtcggacag
     2101 gtgctgcgtg actttctgct cgcatgggaa gccagccagc gcagcgatct ccgcgatggc
     2161 ggtgggcttt gcaaagcggt gggacgactt ttgctgtgtg cagaatcggt cggggcgtcg
     2221 cccaagctgc tcttgcgggc gacgcagcga ttgcaagaac cgacgacgac cgacgaagcc
     2281 gaaccgcagc gagccgcgca agcgggacga attgccgcgg cggccagcct gcttttgcgc
     2341 ctgtccgaaa cctatggacg acgcgctgcc agcgatgcac gactgtttgc tgaagggctg
     2401 cgaaagctcg cattgccccc gcttgtggaa gccgcagccg acatcgaact ggtcgacctg
     2461 cacctggcgc tcgtggcacg cgacgatggg ctcgggctgc cggcctggct cgcggcgatc
     2521 gactgcctgg ccaagaccga ccgcccgctg cggattgtgc tgccctcggt cgacgatcga
     2581 ccgccgctcc agcgtgcgct caagccgatt ttcgattcga tttatcagcg ccacgagctg
     2641 gccatcgaac tgagtgaaag cggctcgctc ggtccatttg ccgaagggac gacgccgtgg
     2701 tcgcgcctga ttcgatcttt gttccgaccc aaaagtggcg gatcgcttgt gtctcctttg
     2761 gaacttgccg atcgagtgac gctgacgcca tccgctggcc cacaggcaga agcgcatgcc
     2821 gtcgctgccc gcgtgcaaaa tcttctgcgc agcggtgttg cggcagcgga cattgccgtc
     2881 gtggccgaat cagccgcgcg caggcgacgg ttgaccgcag cgctgcatcg ggccggcgtt
     2941 cccaccaccc cgctttcagg tggcgtcgct ggcccgcaga cccgcaccgc cgagctgcca
     3001 ccgccgctgc ggctcatttg tcttttgttt gaagtgttaa agctcggtct gccgcgcgaa
     3061 ggctttgttc agcttctgac cagtcgctat gtccgttttc ccggtccgct gggtgacaag
     3121 ccgtggctcg tcgcacaggc cttgcgcacc gccggggtcc gaacccttcg ccatcccgaa
     3181 cgcagccgtc cgtcgacggg aaatctgtgg gaaagcgcgc gcagcgaacc gacctgggga
     3241 ctttgccggc tgcggacctg gctcgccacc cagcaaaagc cagccagcga cgacaaaccg
     3301 cgatcacggc caccgcacct gccgcacctc gtcgagcaag tggaacaggc gctgcacgag
     3361 ctgacgagcc tcccgagcca agccacgatc gccgaccact gccgggccct tgtgcggctg
     3421 cttctgcgcc tgcacttttt cgaaagatgc acgacgctgc cgccgctccc tgaagaaagc
     3481 gaagcggaaa gtgcgacgct gtgtatgcag cttctcgatg cccatcagcg agattctgcc
     3541 tgcgtcggcg tgctctctca gtgtctcgac gagctaccga agtgggcggc acgacttgga
     3601 caagcgacgc tggacatcgg acagcgcgag tttgcccagc tgttgcgcat gacgctaggt
     3661 cgactgtgga acgaagtggc cgaggcgccg cagccgcatg cggtcagcat cggtgaactg
     3721 gcagaaattg ccccccgcac gtttgcgcac cttttcatcg ccggacttat cgacggagag
     3781 ctgccgacct ttcgccccga agactcgctt ttgcccgatg aagaccgacg cttgctcgat
     3841 cgttttgccg accgaccgac gtggccgctt gtgtcgcaac aaaacgacgc cgaaccgctc
     3901 aagctggcgg tcgccctggc gcacgtcgaa cacgcccact tgttctggcc gcttgccgac
     3961 gaagaaggcc gcccgctgcc gcactcgcag tttctcgacg aaatttgttt tgccgcggcg
     4021 attccagacg acaagatcaa gctacggccc gatccgccag cgacgataca tcccaccgag
     4081 ctttggcaac gcgcgcccca acatcaaagc ctgttttcag cgctttcgcg acgagagcgc
     4141 gcacgggcca atcgcctgac ttcgattttg cgcatcgaag aacagcgcag tcgcttcttt
     4201 tccgccacca tcgccgacgc agacgccgca aacttatata gtcatccgtt tgtcggtcgc
     4261 ttgctcgacg acagcttgat caccgtgctg cagccgcggc tgccgggcag tccaaagcat
     4321 ccgctctcgg cgtcggtgct cgaagactat gcgcgctgcc cgtttcgttt tttcgcccgg
     4381 cgcgtgctgg gaattcgccc ggccagcgaa ggaagcgagg agctcgaccc actggcgtcg
     4441 ggacgactgc atcacgcggt gctcgaacat ttttttatcg aacgaaaaga atccaagagg
     4501 ctgccgctgc gtggcgacga agcagacgag gcggcgctcg atgccgcgat ccagcgcgtg
     4561 gtgtccgact ttgaacagac cgagcacatc ggcaatccgg cactattgaa agtccgtctg
     4621 ggacggctgc gcgccgagct gtggcgactt gtcaaaaatg aagcagcaaa gccccccgag
     4681 ccgggctgcc tgccgacgct gtttgagtgg aaatttggcc cactttcgat cgcgtccgcc
     4741 caaggggacg aaggccggat ggcgctgcac atccacggca tcatcgatcg cgtcgatatc
     4801 ggcggcggca aagcggtggt gctcgactac aaggccggac ggctcgatcg ctaccaagcc
     4861 cagcttcgcg acaagctgct aaaaacatcg tttcagctcc cgctttacgt tgcggcactg
     4921 cgcgctgatc cgtcgctgcg cgagagcaca ccgcttcagt cggtcgtcgc ccgctactac
     4981 tcgctgcggc aaggaaaagt gagcaactct ctcgacgacg ccgagatgac cacgcttttg
     5041 cccgaggtgc gactgcgcaa tcccgacggc aacgtcgctg aagtagctta tcggctatgg
     5101 agacggttac gcgacggaga tttcgccgtg gtccccaaaa cctgcgaagg ctgcggactc
     5161 gaaagcgtct gtcgcataag cagtgcgcct ctcgacgttc cacccgagcc cagcgaagaa
     5221 accgcgagca gcgagagcca agaaaacccc gcgccaagcc agctttcacc gacgcgcgat
     5281 tttgacgaag gggacggacg atgagcccac cgctgcggtt tttgcttttg cgcgatcggg
     5341 tggtgcaggc gggggccggc actggcaaga cccacgcgct gctttcgcag tatttgcact
     5401 tgtgtgcagg cgcgacggcg cacggacgac cgattccgcc gcagcaaatc tgtgccctga
     5461 cgtttaccga aaaggccgcc acggaaatgc gcgagcgact ggtccgtcgc gtcaccgcca
     5521 tttgtcggca gcttggggca gttgggtccg aagatgcagc gacggcgctg gctgaaacag
     5581 aacccgacct cgtcgcaagt gctgcgcatc tgggaagcaa gctgcccagc atcgagtcgt
     5641 gggaccaagt tctgtcgagt ctttcgggcg ccaccatcag cacatttcac gctttcgcag
     5701 cggcactgct ccgtcgctac gcccaggtca tcggcgtcga tccagagttt gccctgctcg
     5761 acgaagacag cgcggcacag cgactgcggg aaacctgcga agagctggtc ctttccgcac
     5821 tcgaagaacc gacgacgatt tccagcgacc ctgagtttcc gtcgatcgtc gcgcagcttc
     5881 tcaccgagct cggtttttcc tcgggcatcg gcggcgacgg cggtctgatc gaagttctgt
     5941 gccgtctgca ccggcagctt gccgaagaag ggcaacccgg cgatcggctt acgtcggctt
     6001 acgatcagac gaattttgtg accgcgcttg ccgacgcacg cggcctgctt ttgtcgtctt
     6061 tgcaagaact ggcggcccgc gccgatgaaa ttggtggcaa gcgaggcgaa aaagcggcac
     6121 tgctcggtgg actggccgcg cagattggcc agcgcttggc ttctatcgat gacattgctg
     6181 cgctgcggag cgaatttggc caggtcacgg actgttttcg caccatccgc ggccccagcg
     6241 gtgccaaagc agaccccgtg ctgtcccaga gcatcaaaga ctggaagcag caggccaaag
     6301 acgcaataga cgacctgcaa gcgctgtcgg tgtcacaaaa agccgcgccg ctggccaagg
     6361 gtctggcgct gctcttgggc ccgctcagcg agcgtttttc ccgtctgaag cacgaagaag
     6421 cggtgctcga tttttccgac ctgctttcac tcgcacgcaa ccttctgcgc gatcatccga
     6481 cggtgcgcca cgagctgcgg cagcgttttc aggtcttcct catcgacgaa tttcaggaca
     6541 caagtccgat tcaagccgag ctggtcacgc tgctcgtggg ggatgaagga cattcgccgg
     6601 gccgactgta catcgtcggc gatcgcaagc agtccatcta tgatttccgc ggtgccgacg
     6661 tggcggcgtt tacccggctg tgtcagcgac tccgcgagct gggtgccgat gaggaaacgc
     6721 tcgaccgctc ctatcgctcg ctgccgggag tgatccagtt tgtcaaccgc ctgtttgcgc
     6781 aggtgatgca gccaccgtcg ggtcagctgc ccgattggtt cgtccgctgg gaccccgaaa
     6841 acgatccgct caaagcgcag cgcagcgacg aaacagtggc aacgccccag gtcgagctgc
     6901 tgcgtccgac aaacccagcg ccggccaatc cccaaggcga cgccgtcaag attccgtcta
     6961 tcgtccgcga agccaacctc atcgccgagc gaattgatgc gctgcttgcc gacggagtgc
     7021 ggcctgggca gatcgtcgtc cttttgcggc gcttcaccca tcttttgcat tacacgacgg
     7081 cgctgcgacg gaagcggatt ccccattacg tcgtgcgagg gcgcggtttt tttgcagcgc
     7141 aggaagtccg cgatctcgcg tcgctgctcc gccttttgga cgatcccagc gatgtgcaag
     7201 cgctcgttgc ggtgcttcgt tcgccgctgt gtggtctgtc cgacgaatcg ctggcccgtc
     7261 tgcacctcgc cggtcggctg tctttgccag cactgtgcga aaccccgtcg tcccctggcg
     7321 aaatccctga cgagccagac ctcgatgatc agcccgccca tttgctgctg cgtctgcctg
     7381 ccgacgaaat gaatcggctc cggcacatcg tttcgctgtt gggtctgcta accaccgagg
     7441 ccgaccgttt tgatcccgcc gatcttttca ccatcgccat cgaagcctgt gactaccgcg
     7501 ccgttctcgc cgctgatccc gacggtggac agcggctggc caacctcgac cggctgatcg
     7561 agcgtgcacg ggcctattcg ggtcggctgc gggcatttgc ccgttttttg cgactacaaa
     7621 ccgatcccga tctggctcgt ctaaacggcg atcccagcga cgagccagcg gcgcaaatgc
     7681 tcagcgaaca cgacgacgcg gtgcggatca tgaccgtgca tcaggccaaa gggctcgaat
     7741 ttccaacggt gattgtcgga ggctgcacca cccgcgaacg ccacgacacg ccacaggtcg
     7801 tctacgatcg cgaactgggg ctcggcctgt cgatctatgt cgatggtgag cgacggatga
     7861 cgctgcaaca aaaacgaatc gcaaccaggc gcaagcagcg tgtccgcgcc gagtccgacc
     7921 gcctgtttta cgttgctgcg actcgcgcca aagatcgact gatctgggct ggtgaaagcg
     7981 acagcggcat cggcagcacc tggcgtcagc cgatcgaaca actgatcggc gaagcggata
     8041 ccgcggcgct gtgcaccgag tggagccggg cgacgccagc ccccgaaagc gcagccgagc
     8101 caccgaccaa gcaggtgccg acgactgacc cagcagcagc cgaggagtcg gtccgccttt
     8161 tttacgaacc cccgcttcca ccatcgacaa agaccacgct tgtcaccgcc ctggcagcgg
     8221 aacggctcgc gtgtgctcgc cgctatcagc tcttggccca aagacccgca ccttcgtcgc
     8281 gaccacagca gcgagcgcgg accagcgacg aaaccatgca ccagacggcc caggcctgtc
     8341 tgctcgccgg aacgctagcg cagcggcttt tgtccgtcgt cgatctctcg cgccgtggcg
     8401 aagatgtgtc acgcgcagtc gcccagcttg gaatcttgcg ggacagtgcg ctcgcagcgg
     8461 aagcgcagac ttggctacat acgctgctgc gcagtcgctg gctatccgat gcgcttggcc
     8521 aagccgactg cttttcgtcg ctgcgacgcg ggctttgcta tcacgttccc atcgctacgg
     8581 attcgccgct gtggctttcg ggcgtgctgg atctgacgtg gctgggaacc gacggaacac
     8641 cgcatgtcgt cgacattcaa ctgctgacgc cacccaccaa agagcatgcg ttctttgtcg
     8701 cccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctttgt catcgtgcgc
     8821 agtcgctggc gtaagaaaat cgaagaacag cggtgattac tgctgcattc cgggctgcat
     8881 cggctgcatt ccgggctgtc cgggctgcat cggctgcatt ccgggctgtc cgggctgcat
     8941 cggctgctgc attccgggct gcatccccgg cattccttgg ttcatctggg tgccggtcat
     9001 catctgtagc ttctggaaga gctggttgta cttgccctgc gcctgcgtcg cctccagctg
     9061 ccgccgatcc gccgtcagct ttgcgtcatc cgctttgcgc tgcgcatcgg aagcctgctg
     9121 atcaaacgtc gccaccgaaa agtccgccgt cggcttcttg cccttggccg ccgcaagctg
     9181 cgctttcgac tgttcgtagc gcgaccgcag cgcagaaatc ttcgcttcca tcgcatcgac
     9241 gagcgaccgg gcatggctgc gacgagcctt cgcatagtca atcttggaat ccgccacgtc
     9301 cttcgcgagc tgcttttgct cgaggttgct cttcgcggca ttcatccgat ttaggtcttt
     9361 ggtcgagtcc gccaaatccc gctcggcctt cgccatgttc acgtcggctt tcgccttgtc
     9421 atactcggcg tccgcgacgg tcagctcctt gtcggcaatc gaagcgtcac tgaccgcttt
     9481 gttcttttct tcggtcgcct ggttcaggtc cgactgaatc tgtagcatct gcgacttttc
     9541 acccatcgac acatcggcga gcagcgcatc gtcaatcttg tacttgggac ccgagctaca
     9601 ggcgccaagg agaaccgagg taagcagcac ggaacggaaa agaactcgca tgattcctcc
     9661 agatatcgtc tcactgtaac ctaaccacga aggattgcgg cgccggtccg ctggcccatc
     9721 gcaaggttac gtcggtcttg gcctggtcgt tggtgacggc gactagcttg atgcggtgca
     9781 ggtaaaaccg agcacaagca gccctttgtc atgctgaatg ccggcttgca gcaccgtccc
     9841 gccgttattt atcacaaact tcgtcgacaa gagcttggcg ctgccgtctt ggatctccag
     9901 cttcagacgc agccgaagct tgccagcagc gtcagcccgt ccctcgtagg acagcacgcc
     9961 ttggtgatcg cccggcacct cgaaggtctg cgacgatccg ctcgcaatga ccagctcatg
    10021 ctgccgcaaa agcgcaaagc ctttccacga atcgagcggc ggacgggtaa gctgcggacg
    10081 cagtggctcc aagcgcgtat caaactggcc gccctgcttg gtcgcgtgaa tcacccgcac
    10141 cgcgcacgag gccttcgtcg ctgcatgttg atcaggtgga ttggctttcg acggatcggg
    10201 ctgcgcccaa gcgatctgcg gcaggctcag tgagcacaaa ccgatcgcaa gggcgagcag
    10261 ttgccgtcgt tttttcacag gctttcccat tcgtcttctt cctgatgatc gaaccaaatc
    10321 aaggttgtgt cgttgccatg ctcgtcggca actttcagca cggtcgcccc gccgccgaca
    10381 acttcgagtt cttcgacgtc gcagctattg tcccgcgagg cggaaggtcg cagcagaaac
    10441 aggagcgttg ccgcaagtgc gagcgccgac aacacagccg tcgttcgacg aagcactttg
    10501 cgccgcagcg gcaccacgtt gctcgtcggt ctgtcgtcca gcttgccttc aatttgcgac
    10561 cacacatcga tcgagtgcgc cgccgcttcc gtctcaacag cgtcgtggac agattgcgac
    10621 agcgccgaca gcactgcgag tttcgccagt tcttcgtcag aaagctcacg gcgcagtgct
    10681 tccgcttcgt cgctggttag ctcgttatca aacagcttgt gcagcttgtc atcggtaaca
    10741 gccatggtcg ctcgggccta ttcggccccc tcctgctctg cgtcctcggc gaaatccgaa
    10801 agcagctctt gcatctgccg tcgggcatga aacagtcgac tcatcaccgt tccttctggg
    10861 cagtccatcg aatcggcaat ctctttgtag gataacccct cgacctcgcg caggatgagc
    10921 accgtgcgat gctgatcgga aagctgcgaa agtgcgacga gaatccgctg gcgcagctcg
    10981 ctcgactgcg ccacccgctg tggattgaac gacagcttct gcgccgacag cacatcggcg
    11041 ggactgccaa cctggcgatg ggacaagcgc tcgtcgagct cgacttcgct gccacggcca
    11101 acccggcgct ttcggtcgat gcacagattg acggtgatgc gataaagcca ggtgtaaaag
    11161 ctcgccgtgc cctgaaacga acccaggtgg cgatgcgctt tcaaaaacgc ctcttgcgag
    11221 atgtcgcgcg cttcgtcggg gtctttcagt agcccaaacg ccagcgaaaa tacgcgccgc
    11281 tgatagcgtt cgaccaaaat ccgaaaagcc ttctggtcgc cgcggcgact gcgatcgacc
    11341 agctcggctt cggcgcgtcg ctgctcgtcg gtcgcctcgc tcggcttccc cggctcgacg
    11401 ggtgtcggtg gcgggatgac ttttgcggag ctgtcggttg tctgcgccaa gggtttttat
    11461 cgcttgagtc cgcgccattt gctcctctga cggactcgca gggatctcta tttaaaggag
    11521 cgcctcgcta cgttaacaag aacccagaaa gacgccaatt gcttggtaag ctgcgcgcgc
    11581 tgatgcccga ttcgatcatt cttagttgct tgctgatttt tttcgcccgc gtcggtgacg
    11641 tctctcttgg cacgctgcgt gtcgtcatgg tgacccaagg ccgtcgtggc cgcgctgcga
    11701 tgctcggttt ctttgaagtc ctgatttggg tattcgccgc atcgcgggtc atcggcagcc
    11761 ttggcaagcc acagctcgca gtggcctacg ccgccggttt cgcttgcgga aactacgtcg
    11821 gcctcacgct ggagcggtgg gcagcgatgg gcaatcaggt tgttcgggtt ttctcacgac
    11881 gcggcgaaca gctcgccgac gagctgcgac agcacggctt ccgcgtcacc ctgttccacg
    11941 ggctaggccg cgatggacct gtgcacatgc tctatgtcga aaccgagcgt cgccgcattc
    12001 cgttcattct caacgaagct cgtcggctgg acccgcagtg tttctatgtc gtcgacgacg
    12061 tgcgtatcgc atcatcggtc gacgacccgc cccgcaagcg ctggttatcc gcctacagca
    12121 tccgtcagcg caagtaacgc gacggtcttt cgcaaacgct gcggctactt gtgaagcagc
    12181 ttgaatgcgt gggtccagcg tccacggatc atgccgggaa tgcaccacag catgcacagc
    12241 ggctcgattg cgcgcgccgc cgtcgcatgg cccatgtcct cgatctccca accaaacagg
    12301 tccaggatct tgctcacctg ctctttggcc gccgcgttgt tgccacagat aaacatcgac
    12361 ggcttctgac cgccaaagtc cgggttcacc ataaacgcat tgccgacgca cgaaaacgct
    12421 ttgacaaagt gcgccttggg ggctgctttc tgaatccgct cgagcagcga gtcgtttggg
    12481 ccggtgaaaa acttgagcac accatgttcc ggcgcggctt ccgcgagcgg attggtcgta
    12541 tcgatcacag ttttcccgtc gagcacgctt ccaagcgacg tcaccagctc ctcgacgacc
    12601 gagcccttga cagcgagaac gacgacatca ccaaacgccg cagcttcggc gaacgaacca
    12661 accgatgccc gctctcccgc ttgggtctgc caattcgcca gctttgcgct ctcgcgcgtg
    12721 ccacgcatga cagaaaagcc atgctttagg aaaccatttg ccaggacttc acccacagta
    12781 cccgaaccaa ggacagcaat ttttttcatg gtctgcatgt tagaagcgat cctttccagt
    12841 ggtcaactcg ccgcgattgt cgacgggaac attggaatca ttgcggccat gccagtatcg
    12901 gtcgaggtcc agccgacacc ggatgaaaaa gccaacattc cgttaccctg atatcgagtc
    12961 tacggacaca catgtggcca gccgaattgc ctcattcgag aggaaaatca atcacatcgg
    13021 tcgattagat tgaccattcc aaacaaatgc attccgtatc cacgtaatct ttcttgccgt
    13081 tctttctatc gaaatgacat caaatcgaca ggccactgca ttcatgtgct gacattgttt
    13141 cagtagatat tccattgccg cataatacaa gcgccgctgt ttcgtcacag acacggtctc
    13201 gctcggcgcg ccaaagcgat catcttcgcg caggcggacc tcgacgaaac acagcgtctc
    13261 agcatcgcga gcgacgatgt caagctcacc ccgtcgcgtc cgatagttcc gcgccagcac
    13321 ctccgcccca tgctcgacga ggtagatcgc tgcacgctct tcaccaaaat cgcccaccga
    13381 cttcgagata gacatgaagg ccctctgctg cacggaaatt ctagctgcgc gaccggcatc
    13441 acagcgcatc agctgcgaag acaccgacag caaattccat gtttgaaagc gtagataccg
    13501 acaggaagcc tgcgaataca actcaggcta tggctgcgaa gcgacgtcgc agcgcgcacg
    13561 gaaacggcaa gattggctgg cacagaaaaa acgctgcgcc agcgaaacag tcgaagggct
    13621 agccttcagc agcggcaggc gccggctcag ccgcggcact ctcggtgcgc ttggtcgtgc
    13681 gctccttgat gcgagcggcc ttgcccgaaa gatcgcggag gtagaacaga cgggcgcgac
    13741 ggacttcacc gcggctcacc acttcgacgc gatcgatgcg cggcgagtga accgggaaaa
    13801 ttctctcgac gccgacgccg tagctgattt tgcgaacggt gaacgacgag cgcagaccgt
    13861 ggtgatggcg acggatcaca acgccctcga acgcctgggc acgctctttg tcaccttctt
    13921 tgatcagcac ccacacgcgg accgagtcac ccacgcggaa ctcaggcagc gcctgcttgc
    13981 gcagaagggt cttctctagc agttcaacag cggggacaac atcgctcatc tcaaaattct
    14041 cctacgttcc ggcttcttcg gcgaagagcc tatctaacat gattgccgtc gcagagcgca
    14101 ccgagaggtg attgtacggg gccgttccct gaatcggctc aagcacgcta tcgactttgg
    14161 acataaactc gtcagtgaga ccccaaccag tccccatcac caagaacagc gggcgcgcct
    14221 cgcgaagcag ctgggcgcgg agagcgggta tcgagacggc caacaccgat ggacgagcgg
    14281 ttgttccaac cacgattggc cgctgtccat gctctcgggt aattgcatcg attgcttcat
    14341 ccgtcgtttg gaccacgcgc acgcgcgcaa gggcttctcg acggaaatca ttctgacttt
    14401 gccctgggcc ttcgtgccaa tagcccaaaa ttcgctcgac caactggcgc tgggcagcga
    14461 cgggatgaac gacgtagtaa ccagccacgc cgtatgtgca gcacgatcga gcaatgtcat
    14521 ggatgtcaag gttcgtaact gaagtgctta cgacctgatg ctgcttgtca tacacggggt
    14581 gatgaacaag tgcaacaaat gatcgcgcag ccaaggtcgc gaaaccactg gtagcttgga
    14641 cactcatgtc ggggcctccg agctttcacg caggctgtgt tccgtatccc aagccgcgag
    14701 caagcgccta tcttcgtcgg taagctgaac cgacccaaag aggtcaggac gacgctgtcg
    14761 agtcctcacc agagacagtt gtcgtcgcca gcggcgaatg tgctcgtgat tgcccgagag
    14821 cagcaccgga ggcaccggaa gacccgccac ttccgccggg cgactatatt gtggatattc
    14881 gagcaatccg cattcaaacg actcatcttg tgaagactgc tcgttatgca gcacgccagg
    14941 aatcaaccga gaaagcgcat caaccagtat catggcgggc aattcgccac cagttaagat
    15001 aaaatcacca attgaaatct catcatcgac atagtggtag atacgttcat ccattccttc
    15061 gtatctaccg cacataaaga tcagccgcgg ttccgtggca agctctcgta caagtttctg
    15121 cgtgagcggc tttcccgtcg gtgctaggac aattttgcgc gcctttccat gcgcagcctc
    15181 aatcgactga agtgcccgca ccatcggttc gagcagcatc accattcccg aaccgccacc
    15241 gtaaggcgta tcgtctgtcg tacgatgctt gtccgtcgtg tgatcacgca aattgtgcgc
    15301 acgaaccaca agcgcgcctt tggctatcgc acgacccgtt atgctgacgc gcaagaccgg
    15361 atcgaacatt tccgggaata tcgaaacgat gtcgattgac tgcgccatca gaggctctct
    15421 ggctggtcca gatccaaaag cccagcaggc ggcgagatca ccacttgccg acgatcagca
    15481 tcaaacgacg ccaaaaattc agggatggcc ggtatcaacg ccgactcgcg aggaggacgt
    15541 gcgatgacaa gctgcggctg ggcgccgacc gtcatcacct cctgcacgac gccgactcgt
    15601 tctccatctg tggtcagcac ctcgcagccc ggcagatcgg ccagataaaa ctcccctggc
    15661 tccagttccg gcaggtcggc gcgttccatc agaagtttct gccgggtcag cgcttccgct
    15721 gcggtgcgat cggcaatgcc tgaaaccgac accacaaaaa agccgttcga ctcgccacgg
    15781 cagcttgtga tctgccactg cttgggtcgc tcagcctctt tttctgtcca caaatgcgtc
    15841 gccatgtcga gcgcgttgga cgcagggtca tgcagcttca ctcgaagctc cccccgcaac
    15901 ccatgcggtc gacagactgc accgatctct agaagcgtgc gcgacatcga tttggcgttg
    15961 gtcagatgtg gacagagacg accatccagc gagctgttcg gttcgaagct ccacgacacc
    16021 gccaagtaat ttctatagac aaaagcaagc cggtacccaa gcaacgtcta tccacgggaa
    16081 agacggcgcc taggtcagaa aggaggcttg gtcagtcagg aaatcggcag cgccgcggac
    16141 tcgcggacag tgcgagtgcg atttacttcg acgcagccgc cggagcagcc ttgccggtct
    16201 tcacctggct gatcaggaac gccaccgtgt ccgacggctc agccccttgg ctcagccagt
    16261 agtccacgcg ctccatcttg aagcgaatct ctgccggagt ccgcgtcggg tcgtaaaccc
    16321 caacgtgctc gataaagcgt ccatcacggg ggctacggga gtttgccacc acgacccggt
    16381 agaacggtgc tttcttcgct ccaacacgag ccagtcgcat ctttacggcc atgtttcttg
    16441 gtcctatcta tttcgtctga cggagtcacc tgctaccggg tacggacgaa cccaagcagg
    16501 aatacgtttt gtatgcgacc tgacggaaac tacagaagtt caaggtccgt tcgtctaggt
    16561 cgctcgcgag tcggcaagcc taatggcacg gtgtaggtgc tgtcaagggc ttgaaaacat
    16621 gaccgaagta gcgcgcctcc tgtgagccaa aatcggacga tggattaggc cgcttccttg
    16681 ctgctcgcct cctcatccgc ggtggcggca tcttcggctg gcgtcaccga tcgcgggctg
    16741 acttcaccac ggcgcagcgc agccagcaag cgctcacgca ggctttccat caccacggga
    16801 tgctcggcca gccacagcgc ggcccgctct cgtccctgcg ccagtcgctc cccttcgagc
    16861 gaataccacg ctccgctctt ttcgataagt ccgtgcgaag ccgccaggtc gagcagttcc
    16921 cccgcgcgat tcacgcccac gccatagagc agctcaaatt ccgcatc
//
LOCUS       DAORGN010000100        16897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1379_length_16897_cov_4.772949, whole genome shotgun sequence.
ACCESSION   DAORGN010000100 DAORGN010000000
VERSION     DAORGN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1379_length_16897_cov_4.772949"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            567..2288
                     /locus_tag="PKL17_12185"
     CDS             567..2288
                     /locus_tag="PKL17_12185"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNK45538.1"
                     /translation="MNFAKTCRSLRLWFPALLLFGSLACVPETSHEVATQHESLFVDR
                     TFGSHRYKLFVPSGYSAGRAMPLVMMLHGCTQDPAQFASSTQYNALAEAQGFLVVYPE
                     QPTSEDANRCWSWFQTAHQSRGAGQPQWLAGLVADIGKNYSIDSGRVYVGGLSAGAAM
                     SVILGATYPDVFAAIAVGSGLEFRAASSASDAIAAMRSGGPSPTSQGRTAYRAMGMAA
                     RTVPVIVFHGSSDTTVAPVNADQIVAQWAKTNDLASDGSEDGNITDSADLTSTGTVPS
                     GRSYSRASYRDKRSGSEVMAKVIVQGMGHAWSGGSSAGSFSDPRGPDATALSWEFFRA
                     HPMPTTMPPFDGGTDGGGPVDMRTPSDLAADLATPDLATPDLGVRDLATPDLATPDLG
                     VRDLATPDLALPPTRYVLSVVATESGFVGQVAADGIGPGTAKIGDKGLYNGDTFRAIL
                     SFDTSALPTTAPRSAELVISRKTMTGAVSSLLFDLKRGVFGRDGSLGLDDYAAAPTTA
                     GFASVAPPARDGDRLVIAIPPIVLGMPAAGRFQFRIRASTPVDFKSDLIELWLQSAAA
                     AQLVLTY"
     assembly_gap    2295..2394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2395..3948
                     /locus_tag="PKL17_12190"
     CDS             <2395..3948
                     /locus_tag="PKL17_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45539.1"
                     /translation="PPPFVISLDELKGGLTESVRVDYLGEGDGGLSHTTYYSIVSGSA
                     EYQALPKTEQAKLAKLQSSILHSAKLTPTVQVAGTEITGTLSVQDGAHVRVQEIVLKV
                     PKNWNGSLVVAGTPGTRSELASDAVLAAWLVQKGYAYVAGNKGMTNGGADGNATLLGK
                     NHITASWGMMMHDLALWASTRLQAALGKAPAYTYAVGLSNGGYQVRRALELDDERISQ
                     GQNRRFAGGLDWAGVYFPGAVVLDANRDGKVSPAEYAAAVHLVSSNERAALAMGYLYD
                     PGTVSTPAGYGTNPPFAGAEAAMGKAGFGSPSSILWGTYNIMFDVLKAKFPAWKGVGY
                     YNLTAYYFRADLMGHDAKESAAYSMFSSGSGHPPLYDYLATEKDAGWTDESVSYALRN
                     ANSGTFSVPLVSLHGDKDALIGLPGHGIAYAKAVETSGNAALHRLYVVQHGNHVDAHA
                     DGTLDYDCNGIPGDEGAKDKLTPMQPYVERAFSYLIDWVEHSRPAPASKTIVTDPKSD
                     ILDANQINF"
     gene            4048..4569
                     /locus_tag="PKL17_12195"
     CDS             4048..4569
                     /locus_tag="PKL17_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45540.1"
                     /translation="MKRRWSRCAQLAPLFFTACTSYAIQIHSPPLLLPSGPKGTGQIC
                     VIRPQRVALLVPAVVHDGGKLVGMTQGPSYFCYLAAPGYHRLSTSYGDDIDHRLGTST
                     TQDLTVLVNPSGRIYVHHDVSKILTLSISQLREAEAMQLIAGCDSVSLSSTPNGTPIP
                     NPADVIPAEHPKE"
     gene            complement(4578..5126)
                     /locus_tag="PKL17_12200"
     CDS             complement(4578..5126)
                     /locus_tag="PKL17_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK45541.1"
                     /translation="MTAHRKARNGLPVPHSLSILQEQISRRCAATSAAHPFWPCASGC
                     DDCCRSLAAVPSLHHVEWDALWSAIVALPADQLAQVSREMDALCEKESAGLCGSLVCP
                     LLDQTQGICRVYPARPLACRTYGFYVDGADGKYCGKVTAALQQNPDAEVVFGNEPSVL
                     KDAERAFGKTRTLTEFWRQRAE"
     gene            5188..7332
                     /locus_tag="PKL17_12205"
     CDS             5188..7332
                     /locus_tag="PKL17_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK45542.1"
                     /translation="MSARRRLRNRWLVLILGVSCVQLGCAAARDIDDVEEGPGPGRPC
                     EHDSDCKTGRRCISHRCHPDYGDCTSDNDCENDTACACPPDLQTERCACIPWGMTPRG
                     RSDDSCSGAGFPIDDFQSPVVKCQWPPKGAMPPAYKDVLGTPVVIDLDGDGETEIVFA
                     AGYAGTTHLVALSGKDCSVRFDKPTPGPGCSNVGAADLDGDGKAEVVAVASGVTVFDS
                     TGSVLTSRSEIGAGLLCVRDYAIAFANLDGEGPPEIIVGAAAFRYTSSPKPQLQLLWN
                     KNLIEEGAWGTIPAVADLDGDGKSEVITGHHVFDGITGADKTPSVMKNLGGGYAAIAD
                     FNADGKPDIALVSSKMDDQNVSIIDYQANRLILAPQHAQNGWGGAPTIADFDGDGRPE
                     IGTAGASYYYVYSPDCAMSPLPAKCQGSDDGVLWQSETQDLSSGSTGSSVFDFNGDGE
                     AEVVYRDECWLRVFSGRSGKKLFAAPITSGTVLDLPVIADVDGDGHAEIVVASDASQN
                     DACRTGKWKTELGITHPGATSGIRVLEDPKDRWATARPLWNQHSYHITNILDNRQIPL
                     RETESWKAHNTYRQNLPQSKAGKESQPDSTGRIEFPPDVGDCATLLRLSGVVCNRGAQ
                     TIPKGFPSTFYLGDPRLRSARSLCTADTDRPVAAGDCVAVSCEWKNPLLPPYDLWFVT
                     NDDGRGGHPLAECKNGNNLSHKTFADCPSVPK"
     gene            7360..10272
                     /gene="polA"
                     /locus_tag="PKL17_12210"
     CDS             7360..10272
                     /gene="polA"
                     /locus_tag="PKL17_12210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNK45543.1"
                     /translation="MSSLYLVDGSNFLFRAFHAMPMMVSSKGVPTGAVRGFASMLLRL
                     IGEHKPTHLAVVFDAGGKDKRAERFPAYKANREECPAELKPQFDLASQLTEAMGVVCL
                     QARDAEADDVIATLSRRAEQAGEQVVIVSSDKDLMQLCRDGHITLLDTMKEEGRGKLF
                     GVAEVIEKWGVGPDQLGDVLALMGDASDNLPGIPGVGPKTAAQLIQTFGSLDELIRRC
                     DEITVRGKDKIIAAIKEHEAQLRLVRELVALDEKLDGLPQVSALCRQTVDPVLLLRLL
                     KELEFTTLYRRLVAPGGMPGMPDLSAAAQAIESGTAASPAKPAPVIATPEKATTAATE
                     HSADAPKPSAPSTQPSFPLTDAIQHTAGAIQIITDEAGLVRAIDELVAKLGPSPLVAV
                     VPAWCEDGHSGHNARLSPLCGLALCTASAPPIYIPFAHRYLGAQSQPSRDAVLGKIGP
                     LLRDEKIAKAVYGAKETQQALSGLGISLGGIVTDPALCSYLLDAGEAHALSDLAAKHL
                     PPDYPKLILRKAVCQSGKHALLLDAVEIPRAAELVGGEARATLLLGGFLRSTLDAASQ
                     LLLQTLELPLSTVLATIEGHGIALDTAVLAKLSSEVATRLDQLEAEIERVSGTQVNLN
                     SPKQLAELLFEKLGLPPVKKTKGKTGLSVDAEVLEALAADFPVAQQILEYRSLQKLKG
                     TYIDALPLLVSKTTGRLHTSYQQVVAATGRLSSTDPNLQNIPVRSELGQKIRAAFVAA
                     PGHLLIAADYSQIELRVLAHLSADPLLVDSFRSGEDVHTRTAIEMFGQEEGKHPDKRR
                     AAKMINYGIVYGLTDYGLATRLGIERKLAKRYIEEYFARYHGVRSFMDELVKRARQDG
                     GARTLRGRFRPLPDLTSKNFPARGYAERMAKNTPIQGTAADILKQAMIDVQRVLGEKE
                     PTTKMLLTVHDELVLEAPLDRKDAVCTLLKDTMEKAETLSVPLTVDVGAAQTWADC"
     gene            complement(10290..11411)
                     /gene="galK"
                     /locus_tag="PKL17_12215"
     CDS             complement(10290..11411)
                     /gene="galK"
                     /locus_tag="PKL17_12215"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNK45544.1"
                     /translation="MSSKLPHLPTRFQQMFGVAATARATAPGRVNLIGEHTDYNDGLV
                     LPTAIPQRTEVSLALADDGVVSVYSDATSGDGPASSYRLGEESRHGDWLDYVRGVTWV
                     LRQEGLLTRLSGLRLAISSQVPVGSGLSSSAALLVSLLRGLRIALALPISEIDLARLA
                     RRAEVELVGAPVGIMDQLACALCGPGAALLLDTQSLQMDTVPIPSSLELVVIHSGIVH
                     SHAAGDYKTRRRECQLAAQQLGVTSLRQLGEADLPRIMELPDPLGRRVRHVVTENARV
                     QTAAVALAAGDGETLGRLFAASHASMRDDYEVSVPAVDQLVEIGAADPAIWGARLTGG
                     GFGGSVVMLAREGEGRAAAIRIAEKYRSHVGHNPTVLVP"
     gene            complement(11408..12139)
                     /locus_tag="PKL17_12220"
     CDS             complement(11408..12139)
                     /locus_tag="PKL17_12220"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNK45545.1"
                     /translation="MISKARGLLLSGLSILWGGSLLGSFAVLPAAEPGVGRVPLPLRL
                     LSSATLVLASWVIRGGKRKGPAKQFGHQIAKGMTWGFIGDVCMLVSVPLGMLTFGIGH
                     IEYSRGMWRLGNEEGVGRGRRLLAWAAWMAIGIAGYVFVVHMGPKGGTMLAWAALPYT
                     LLLSTTAGLASGLFLHKARYFPLALGAALFLASDLLIAMRMFHPDLFAQIPDWIRGDL
                     VWLLYGPGQALIVSSVLWASEEEQP"
     gene            12266..12634
                     /locus_tag="PKL17_12225"
     CDS             12266..12634
                     /locus_tag="PKL17_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45546.1"
                     /translation="MELAPLCVPVEIAVRNGALRCFRLALAVSDTGLRLRSPLPDELL
                     REPLRIRLTLPPPTEELARLFADEWDGNLIVFARAAAEVVAPNTPNEKRLWRLLVFEK
                     PDPAMREKIQRYVDLRTDAA"
     gene            complement(12638..15661)
                     /locus_tag="PKL17_12230"
     CDS             complement(12638..15661)
                     /locus_tag="PKL17_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNK45547.1"
                     /translation="MMSAWRNFLFALAMLCGLGLSPSHAQESGRALVRSVPLPPGSSA
                     PPLLPDEYSRLGSGRLSMAAKTQMFRFLGVLPGSASQDEAGRLRLLVQTTDGGKSLRA
                     AGLNVTPLSQTIAQVSVDKSALRTLLENPQVRRIDGPHRWRPLLDQSRALIGGQALTD
                     KFGLTGKGVMVGIVDTGVDFRHADLRDKAGQTRIAYLISRSERRDGRHPEFPDYDGMQ
                     IVTAQDINAVFDAEAKGMQPAFAIRETDINGHGTHVAGIAASSGLATTAKFAAGRYVG
                     IAPESTLCIAKATADEVSFEDADILLGVRFCLDRADADKLPLVVNLSLGSDGGSHDGS
                     SLIEVALDEMFAQRPGRILVAAAGNSGDLDIHSSSSLFHGTHDITIHIDHADIPVQES
                     TTSLELYYDAAAPPLRDGTANLTLTLRSPSGRRFEVPMGQSLQASFPDEGEAVIDASD
                     VAETGLRGVLVSIFKPAGQGYPKAGDWHLEIQGQTRRYDLWKVFSSDDLDVSLRGHLD
                     PDGYLAIPASARSVISVGAQRSRLSWTRADGKLASFDRELNRVATFSSGGPLRDGRFA
                     PDVLAPGEFIVSALSSGTIQSDPRSSFALPHDSGLLIADDGLHGVLRGTSQAAPHAAG
                     AVALLLQFSPGLLTNDVRELLRTTSLLPNFQGFGPRHGFGQIRLENALARLQNAPRYS
                     LSALDSDVGVNRDIAAPGVDTVTVTVTPRDVLGQPLGPGLPVMISADHGEWLDSVRDE
                     GHGRYERTLLSTGPRGSQAKIEVRVDGILLNQQPVVYFVGERSEIGQPFVIGACSMGP
                     RPSSPVAVFVVLLLPLLGLWRRRFGAVLGSACLVLGFVGCSDGRDSVGSVKFSSRDKG
                     GDFFWAVHDDLLHPHLVIHLQTQKADLFEGAELVAQSSICSGRQSHKTPKGSYSVLEK
                     VPLYVSGRYGDYVTEMGEVLLSNVDNHDQTPPPGTTFRGTPMPYFMRIFGGIGLHAGP
                     LVGHPDSHGCVRFPQRFAQRLFDAVPLGTKVDVVD"
     gene            15747..16775
                     /locus_tag="PKL17_12235"
     CDS             15747..16775
                     /locus_tag="PKL17_12235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNK45548.1"
                     /translation="MAKLRRLLLRPRRSLGYLRAMSIYLVTGGCGFIGSNLVHALLQR
                     GDEVRILDNLSSGRFSNIGKLLDSHPRQVSLWRGDILDGPLLDRVMHGVEYVAHLAAM
                     PAVTWSVAQPIWCDRINSGGTLQVLDAARRQGKVRRVVIAASCAAYGDLNPTEPKRES
                     DEVAPMSPYAAAKLASEHHGSVYSQIYGVPTVSLRFFNVFGPRQDPSSQYAAVVPNFV
                     RAALENKRPKIFGDGKQSRDFVFVDNIVSAIRLAWTCDERRVAGQVMNIGCGEQVTLL
                     DLVAELEKLCGRSLNPEFLPAREGEVRHSFADIARARSLLGYSPEVRFPEGLARTLRW
                     FAQEKDAA"
BASE COUNT         3516 a         5131 c         5018 g         3132 t
ORIGIN      
        1 cctcgatctt gccttgctcg gttccctcga cggtcatatg cgcttgcagt gccatggtcc
       61 ttgctcctta tgttgaaaaa gagacagctt tgcccgttcc ggactgtctc gtgaacagct
      121 gcacgttgtg ctgcgatcga agtccgtttg gataggactg agtccaacat gaagcggact
      181 cagcttgagc gcttggctgt tttgacagcg ctggcagcgc agactcgcac gacggtagag
      241 gcgacgaact catcgcaaaa acctccaaac agaggaccga ccgcagaagc cttccccaca
      301 taacaaggcc ccttcccaat tgcaagcttt ggttctcaat tttggttcgc caggaaaatt
      361 gctcatgcag ccgtggaaac ggacagatcg caaagtccat tccgtacttt tgccaaaatc
      421 gacttcatgc tggccttcgc tttccgcttt gcagatcgcg tgcacccgaa atgggaagga
      481 aaggcccgat tccagcttga cgaaacttgc ggtgccgcac tacgcaatcg ctccgcagcc
      541 cacgtccgct gcccctggag aaacaaatga acttcgcaaa gacttgccgc tcgctgcggc
      601 tttggttccc tgccctgctt ctgttcggct cactcgcgtg cgtccccgag acatcgcatg
      661 aagtggcgac gcagcatgag tctctgtttg tcgacagaac gttcggaagt caccgctaca
      721 agctgtttgt gccgtcggga tattccgccg gacgcgcgat gccgcttgtg atgatgctgc
      781 acggctgcac ccaagatccg gcgcagtttg ccagctcgac acagtacaac gcgctggcgg
      841 aagcgcaggg ctttctggtg gtctatccgg aacagccaac aagcgaagat gccaaccgct
      901 gctggagctg gtttcagacc gcgcatcagt cgcgcggcgc gggacagccg cagtggctcg
      961 caggacttgt ggccgacatt ggcaaaaact acagcatcga ttctggccgc gtgtacgttg
     1021 gtggtttgtc ggcgggcgcg gccatgtcgg tgatcttggg cgcgacctat cccgatgtgt
     1081 ttgccgcgat tgccgtcggt tccggtctgg aattccgcgc tgccagttcc gcaagtgatg
     1141 cgattgcggc gatgcgcagt ggtggaccca gtccaacatc acaaggccgc accgcgtatc
     1201 gcgcaatggg aatggccgcc cgcacggtgc cggtgattgt gtttcatggc tcgtctgaca
     1261 caacggttgc cccggtcaat gccgatcaga ttgttgcgca gtgggccaag accaatgacc
     1321 tggcgtccga cggcagcgaa gacggcaaca tcaccgacag cgcggacctg accagcaccg
     1381 gcactgttcc gtctggtcgc agctacagcc gtgcaagcta ccgcgacaaa cgatccggca
     1441 gcgaagtcat ggccaaagtc atcgtgcaag gcatgggaca cgcatggtcg ggcggcagca
     1501 gcgcgggttc cttttccgat ccgcgcggac ccgatgccac cgcgctgtcg tgggagttct
     1561 tccgcgccca tccgatgcca accacgatgc cgccgttcga cggtgggacc gatggtggtg
     1621 gacccgtcga catgcgcact ccttctgact tggcagcgga tctggcgact cccgatctcg
     1681 cgactccgga tcttggtgtg cgtgacttgg cgactcctga tctcgcgact ccggatcttg
     1741 gtgtgcgtga cttggcgact cccgatcttg cgcttccccc gacgagatat gtgctttccg
     1801 tggtcgcaac agagagcggc tttgtcggtc aggttgctgc cgatggaatc ggtccgggaa
     1861 cggccaaaat cggtgacaaa ggactgtata atggtgacac cttccgcgcg attctgtcgt
     1921 ttgatacctc ggcactaccg acgacagctc cgcgcagtgc agagcttgtg atttcgcgca
     1981 agaccatgac tggcgcagtc agctcgctgc tgttcgatct gaagcgtggg gtgtttggtc
     2041 gcgatggatc gcttggcctg gatgactatg cagcggcccc aaccacagcc gggttcgcca
     2101 gcgtagcgcc acctgcacgc gacggagaca ggctggtgat tgcgattcct cccatcgtgc
     2161 tgggaatgcc cgccgcgggt cggtttcagt ttcgcatccg tgccagcacg ccggttgatt
     2221 tcaagtccga tctgatcgaa ctgtggctgc aaagtgccgc agcggcgcag cttgtgctga
     2281 cgtactaacg gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgccg
     2401 ccgtttgtca tttcactgga tgaactcaaa ggcggactga ccgaatcggt gcgagtcgac
     2461 tatctgggtg aaggcgatgg gggactgtcc cataccacct attattcgat tgtgtctgga
     2521 tcagcggaat accaagcgct gccgaaaacg gaacaggcca agcttgccaa actgcaatcg
     2581 agcatcctgc acagtgcaaa gcttacaccg acggtacaag tcgccggaac agagatcacc
     2641 ggaacccttt cggtgcaaga cggagcccac gtccgcgtgc aggaaatcgt cctgaaggta
     2701 cccaagaact ggaacggatc gctcgtggtg gcaggaaccc ccggaacgcg cagcgagctg
     2761 gcaagtgatg ccgtgctggc cgcgtggctt gtccaaaagg gatacgctta cgttgcgggc
     2821 aacaaaggaa tgaccaacgg aggggccgat gggaatgcca cgctgcttgg gaaaaatcac
     2881 atcaccgcat cctggggaat gatgatgcat gatctggcgt tgtgggcttc cactcgtctg
     2941 caagctgcgc ttgggaaagc ccctgcatac acctatgcag tcggtctttc aaacggaggc
     3001 tatcaagtcc ggcgcgcgct cgaactcgac gatgagagga tttcacaagg acagaaccgc
     3061 cgttttgcag gaggactcga ctgggccggc gtgtactttc cgggtgcggt tgtgctcgac
     3121 gcaaaccgtg atgggaaagt gtccccggca gaatacgccg ccgcggtgca tttggtgtcc
     3181 agcaacgaac gcgccgcgct cgcgatggga tatctgtacg atccaggtac ggtgtcgacg
     3241 cctgctggct atggaacgaa tcctccgttt gccggtgcgg aagctgcgat gggaaaggca
     3301 gggtttggca gtccatcgtc gattctgtgg ggcacctaca acatcatgtt tgatgtcctg
     3361 aaagcgaaat tcccagcatg gaaaggagtc ggatactaca acctgaccgc gtactatttt
     3421 cgtgccgact tgatgggaca tgatgcaaag gaaagtgcgg catacagcat gttttccagc
     3481 ggcagcggac accctcctct gtatgactac ttggcgacgg aaaaagacgc cggatggacc
     3541 gatgaaagcg taagctatgc cctgcggaat gcgaacagcg gaaccttttc tgttccgctt
     3601 gtcagcttgc atggtgacaa agatgcgctg atcggtcttc cgggtcatgg aatcgcttac
     3661 gccaaagcgg tcgagaccag cggaaacgcg gcgctgcacc gcttgtatgt ggtgcaacat
     3721 ggcaatcacg tcgatgcgca tgccgacgga acgctcgatt atgactgcaa cggaattccc
     3781 ggtgatgaag gagcaaaaga caagctcacc ccgatgcagc cgtatgtcga gcgcgcgttt
     3841 tcctatctga tcgactgggt ggaacacagc cgaccggccc ccgctagcaa gacgattgtt
     3901 accgatccaa agagcgacat cttggatgca aaccagatca acttctgatc gggacacgac
     3961 ggaacgccac gacggaaacc ggcgcagcca ttgacgctgc gtctgcggtc agtgtacgac
     4021 tccagtgatt tttcactggg gttttcgatg aagcgtcgat ggtcacgttg tgcgcagctt
     4081 gcaccgcttt ttttcaccgc gtgtacaagc tacgcgattc aaattcactc gccgcccctg
     4141 cttttgccaa gcggtccaaa aggaaccgga cagatttgcg tgatccgtcc gcagcgggtc
     4201 gcgctgttgg ttccggcggt cgtgcatgat ggcggaaagc tcgtcggaat gacgcaagga
     4261 ccatcgtatt tctgttacct ggcggcgccc ggatatcacc gcttgtcgac gagctatggc
     4321 gatgacatcg atcatcgcct gggcacgtct accacgcagg atctgaccgt gcttgtaaac
     4381 cccagcggtc gcatctatgt gcatcacgat gtgtcgaaaa ttctgacgct gtcgataagc
     4441 cagctgcgcg aagcggaagc gatgcagctt atcgctggct gtgactctgt ttcgctgtcc
     4501 tcgacgccaa acggaacgcc gattcccaat cccgccgatg tgattcccgc ggaacatccc
     4561 aaagagtaaa atggctgtta ctccgcacgc tgccgccaaa actccgtcag cgtccgcgtc
     4621 ttcccaaacg ctcgctcggc gtctttgagg accgaaggtt cgttgccgaa caccacttcc
     4681 gcgtccggat tctgctgaag cgcagcggtc acttttccac agtattttcc gtcggcccca
     4741 tcgacataaa agccataggt tcggcaggcc aacggacgcg ccggatagac ccggcagatt
     4801 ccttgcgttt gatcgagcag cggacagaca agcgatccac aaagaccagc ggattccttt
     4861 tcacacagcg cgtccatttc acggctcacc tgcgcaagct gatccgctgg gagcgccaca
     4921 atcgctgacc acagcgcatc ccactcgacg tggtgcagac tggggactgc ggcaagcgaa
     4981 cgacaacagt catcacaacc ggatgcgcag ggccagaacg gatgcgcagc ggatgtcgca
     5041 gcacagcgac gagaaatttg ttcctgcaag atcgaaagcg agtgcggcac aggcagaccg
     5101 ttccgcgcct tgcggtgcgc tgtcaagtga tgactgcatg cacaaggcac ctgttgactg
     5161 ccccaagatg ccgttgtacg aaaccgcatg agtgcgagac ggcgactgcg aaacagatgg
     5221 cttgtactga tcttgggtgt cagctgtgtg cagctcggct gcgccgccgc gcgtgacatc
     5281 gacgatgttg aggaaggccc cggccccggt cgtccctgcg aacacgacag cgactgcaaa
     5341 accggacgcc gctgtatttc gcaccgctgc catcccgact atggcgactg cacaagcgac
     5401 aatgactgcg aaaatgacac ggcgtgcgcg tgtcctcccg acttgcaaac agagcgctgc
     5461 gcctgcattc cgtgggggat gacgccgcgt ggtcgcagcg acgacagttg cagtggcgcg
     5521 ggattcccaa tcgacgattt ccaaagccca gttgtcaagt gtcagtggcc gccaaaaggt
     5581 gccatgccgc cagcatacaa agatgtgctg ggaacccccg ttgtcattga tctcgacgga
     5641 gatggcgaaa cagagattgt ctttgcggct ggttatgcgg ggactacgca cctggttgcg
     5701 ctcagtggaa aggactgttc ggttcgtttt gacaagccaa ccccagggcc cggctgtagc
     5761 aacgtgggag ccgcggatct cgacggggac ggcaaagccg aagtggtggc cgtggcaagc
     5821 ggagtcaccg tgtttgacag cacaggttcc gtcctgacca gtcgatcgga aattggggct
     5881 ggacttttgt gcgtgcgcga ttatgcgatt gcgtttgcca acctcgacgg ggaaggaccg
     5941 ccagagatca ttgttggtgc ggcagcattc cgctacacaa gcagtcccaa gccgcagctt
     6001 cagcttttgt ggaacaaaaa tctgatcgaa gaaggcgcgt ggggaacgat tcctgctgtc
     6061 gctgatctcg acggagacgg caagtccgaa gtcatcaccg gacaccatgt gtttgatgga
     6121 atcaccggtg cggacaagac gccttcggtg atgaaaaatc tgggtggagg atacgcagcg
     6181 attgccgatt tcaatgcaga tgggaagccg gacatcgcac tggtcagctc gaaaatggac
     6241 gaccaaaacg tatcgatcat cgactaccaa gcgaatcgtc tgattctggc tccgcaacat
     6301 gcacagaacg gctggggagg cgcccccacc atcgctgatt tcgacggaga tggtcgtcca
     6361 gaaatcggca cggcaggtgc ttcttattac tatgtctaca gtccagactg tgcgatgtca
     6421 ccgcttcccg ccaagtgtca aggaagcgat gacggcgttc tgtggcaaag cgaaacacaa
     6481 gatttgtcgt cgggaagtac cggatcgtcg gtgtttgact tcaacggcga tggcgaagcg
     6541 gaagtggtct atcgcgatga atgttggctg cgggtgtttt ctggacgcag tggcaaaaag
     6601 ctgtttgcag caccgattac atcgggaacc gtgcttgatc tgccggtgat tgccgatgtc
     6661 gatggggatg gtcacgccga aattgtcgtt gcatcggatg ccagtcaaaa cgatgcgtgt
     6721 cgaaccggaa aatggaaaac agagctagga atcacgcacc ctggcgccac gtctggcatt
     6781 cgtgtgctgg aagatccgaa agaccgctgg gcgacggcaa ggccattgtg gaatcagcat
     6841 tcctatcaca tcacgaacat cctcgacaac cggcagattc cgctgcggga aaccgaaagc
     6901 tggaaagccc acaacacgta ccggcagaac ctgccacaga gcaaagcagg aaaggaatct
     6961 cagccggact cgacgggaag aatcgaattt ccacccgatg ttggggactg tgcaacgctt
     7021 ttgcgcttgt ccggggtggt gtgcaaccgc ggtgcgcaga cgattcccaa aggattccca
     7081 tcgacgtttt atttgggcga tccacgccta cgcagcgccc gatcactctg caccgcagac
     7141 actgatcgtc ccgtcgctgc gggcgattgt gttgcggtgt cttgtgaatg gaaaaacccg
     7201 ctcttgccgc cgtatgatct gtggtttgtg acaaacgacg atggcagagg cggacatcca
     7261 cttgcggaat gcaaaaacgg caacaacctt tcgcacaaaa cgtttgccga ctgtccttcc
     7321 gtcccgaaat agtcacgcgc ttctgctagt ctgcgcgcca tgtcttcgct ttatctggtg
     7381 gacggttcta actttctgtt tcgtgccttt catgcgatgc cgatgatggt gtcgtcaaaa
     7441 ggcgtgccga cgggtgcagt gcgtggtttt gcgtcgatgc tgctgcggct gattggtgaa
     7501 cacaaaccga cgcatctggc ggtggtgttc gatgctggcg gcaaggacaa gcgggcggaa
     7561 cgcttcccgg cgtacaaagc gaaccgcgaa gaatgccccg ccgagctgaa gccacagttt
     7621 gatttggcgt cgcagctgac cgaagcgatg ggcgtggtct gtctgcaagc gcgcgatgcc
     7681 gaggccgacg atgtgattgc gacgctttcg cgccgggccg agcaagcggg tgaacaagtc
     7741 gtgattgtgt cgtcggacaa agacctgatg cagctctgtc gcgatggcca catcacgctg
     7801 ctcgacacga tgaaggaaga aggccgcggc aagctgtttg gcgtcgcgga agtgattgaa
     7861 aagtggggcg tgggcccgga tcagctcggc gatgtgctgg cgctgatggg cgacgcgagc
     7921 gacaacctgc ccggcatccc cggcgtcggc cccaaaacgg cggcgcagct gatccagacg
     7981 tttggttcgc tcgacgaact gattcgccgc tgcgacgaaa tcacggtgcg cggcaaagac
     8041 aagatcatcg ccgcgatcaa agagcatgaa gcgcagcttc gcctggttcg cgagctggtc
     8101 gcgctcgacg aaaaactcga cggtctgccg caggtgtcgg cgctgtgtcg acagactgtc
     8161 gatccagtcc ttttgctgcg gcttttgaaa gagctggaat ttacgacgct gtatcgtcga
     8221 ctggtggcgc ccggtggcat gccgggcatg ccggatctgt cggcagcggc gcaagcgatc
     8281 gaatcgggga ccgccgcaag cccggcaaag ccagcgcccg tcatcgccac acctgaaaaa
     8341 gccacgaccg cagccacgga acattcggca gacgcaccga aaccaagcgc ccccagcacg
     8401 cagccgagct tcccgctcac cgacgcgatc cagcacacag cgggagcgat tcagatcatc
     8461 acggatgaag ccggtcttgt acgagccatc gacgagcttg tggcaaaact tggcccgtcg
     8521 ccgcttgttg ctgtggttcc ggcgtggtgt gaagacggcc acagtggtca caatgcccgt
     8581 ctgtcgccgc tgtgtggact ggcgctgtgt accgcgtcgg caccgcccat ctacattccg
     8641 tttgcacacc gctacctggg ggcccagtcg cagccgagcc gcgacgcggt gcttggcaaa
     8701 atcgggccgc ttttgcggga tgaaaaaatc gccaaagcgg tctacggagc caaggaaacc
     8761 cagcaagcgc tgtcgggact tgggatttcg ctgggtggaa ttgtcaccga cccggcgctg
     8821 tgttcgtatc tgctcgacgc gggggaagcg cacgcgcttt ccgacttggc ggcaaaacac
     8881 ctgccgccgg attaccccaa gctgattctg cgcaaagcgg tttgtcagtc gggcaagcac
     8941 gcgctgctgc tcgatgcagt cgaaattccg cgtgcggcag agctggtcgg gggcgaggcg
     9001 cgggcgacgc ttctgctcgg tggttttctg cggtctacgc tcgatgcggc gtcccagctg
     9061 ctgttgcaga cactggagct gccgctgtcg acggtgcttg cgacgattga aggccatggg
     9121 attgcgctcg ataccgcggt gctggccaag ttgtcgtcgg aagttgcgac ccggctcgat
     9181 cagctagaag ccgagattga gcgggtgtcg ggcacgcaag tgaacctcaa ttcccccaag
     9241 caactggctg agctgctgtt tgaaaaactc ggtctgccgc cggtaaaaaa gaccaaaggc
     9301 aagaccggac tgtcggtcga tgcggaagtg ctggaagcgc tggctgcgga ttttcccgtc
     9361 gcacagcaga ttctggaata ccgcagcttg caaaagctca agggcacgta catcgacgcg
     9421 ctgccgcttt tggtcagcaa gacaaccggg cgactgcaca cgtcgtatca gcaggtggtc
     9481 gcggccactg gacggctttc gtcgacggac ccgaacctgc aaaacattcc ggtgcgcagt
     9541 gaactgggcc aaaaaatccg cgcggcgttc gtggcggcgc ccggacatct gctgattgcg
     9601 gcggactatt cccagattga gctgcgggtg ctggcgcatc tgtcggcgga cccgctgctc
     9661 gtcgacagtt tccgttccgg tgaagacgtt cacacccgca ccgccatcga aatgttcggc
     9721 caagaggaag gcaaacaccc cgacaagcga cgggccgcca agatgataaa ctacggcatc
     9781 gtctacgggc tcaccgacta tggtttggcg acgcggctgg gcatcgaacg caagctggcc
     9841 aagcgctaca tcgaagaata tttcgcgcgc tatcacgggg ttcgcagctt catggatgaa
     9901 ctggtcaagc gcgcacggca ggatggtggc gcacgcacgc tgcgtgggcg attccgaccg
     9961 ctgccggact tgacgagcaa aaacttccca gcccgcggct atgccgagcg gatggcaaaa
    10021 aacacaccga ttcaaggcac cgcggcggac attctcaaac aagcgatgat cgatgtgcag
    10081 cgggtgctcg gtgaaaaaga gccgacgacg aaaatgctgc tcacggttca cgatgagctg
    10141 gtgctggaag cgcctcttga ccgcaaagac gccgtctgca cgctgctcaa agacacgatg
    10201 gaaaaagccg agacgctgtc ggttccgctc acggtcgatg tcggcgcggc ccaaacctgg
    10261 gccgactgct aaccacgcaa gatgggcggt tacggcacca gcactgttgg attgtgtcca
    10321 acatggctgc ggtacttttc ggcgatgcga atggcggcgg cgcggccctc tccttcgcgc
    10381 gccagcatca ccaccgatcc gccaaaacca ccgccggtca gccgagcccc ccaaatcgcc
    10441 ggatctgccg cgccgatttc gaccagctga tccaccgcag gaaccgacac ctcgtagtca
    10501 tcgcgcatcg acgcatgcga cgccgcaaac agccgtccga gcgtttcccc atcgcccgcc
    10561 gcaagcgcca ccgcggctgt ctgaacccgt gcattttccg tcaccacatg gcgcacccgt
    10621 cgacccagcg gatcgggcag ttccatgatg cgcggcagat ccgcttcacc aagctgccgc
    10681 agcgacgtca ccccgagctg ttgtgccgcc agttgacact cgcgccgccg ggtcttgtag
    10741 tcgcccgccg cgtggctgtg cacaatcccc gagtgaatca ccacaagctc cagcgacgat
    10801 ggaatcggca ccgtgtccat ctgcagcgat tgggtatcga gcaaaagcgc cgcccccgga
    10861 ccacacagcg cacaggcaag ctgatccatg attccgacgg gcgccccgac cagctcgact
    10921 tcggcacgac gcgccagccg agccagatcg atttcagaaa tcggcagcgc cagcgcgatt
    10981 cgcagccccc gcaaaagact gaccagcagc gccgcgctcg acgacagacc gctacccacc
    11041 ggcacttgtg acgaaatcgc cagccgcagc ccagaaagtc gcgtcagaag cccttcttgc
    11101 cgaagcaccc acgtcacacc gcgcacgtag tcgagccagt cgccgtgacg agactcttcg
    11161 cccagtcgat acgacgaggc cggcccatcg ccgcttgtcg catcgctata gaccgacacc
    11221 acgccatcgt ccgcaagcgc cagactgacc tcggtacgct gcggaatcgc cgttggcagc
    11281 accagtccgt cgttgtaatc ggtgtgttcg ccgatcaagt tcacacgacc aggcgccgtc
    11341 gcacgcgccg tcgctgcgac gccaaacatc tgctgaaacc gcgtggggag atggggaagc
    11401 ttgctgctca tggctgttcc tcttcgctcg cccaaaggac gctcgacaca atcagcgctt
    11461 ggccgggacc gtacaaaagc cacaccaagt cgccccgaat ccagtccgga atctgcgcaa
    11521 acagatcggg atgaaacatt cgcatcgcga tgagcaggtc cgacgccaaa aacagcgccg
    11581 ccccgagcgc gagcggaaag taacgcgctt tgtgcaaaaa gagcccgctt gccaagcccg
    11641 ccgtcgtcga caaaagcagc gtgtaaggca gcgccgccca cgccagcatt gtgccgcctt
    11701 tgggccccat atgaaccacg aagacatagc ccgcgatgcc aatcgccatc caggccgccc
    11761 acgccaaaag ccgccgccct ctgcccacac cttcttcgtt gccaagccgc cacatcccgc
    11821 gcgaatactc gatgtggccg atgccaaacg tcagcatgcc aagcggcact gagacgagca
    11881 tgcagacatc cccgatgaaa ccccaggtca tgcctttggc gatttggtgg ccaaactgtt
    11941 tcgcgggccc tttgcgcttg ccgccgcgaa tcacccagct tgccaacacc agcgttgccg
    12001 acgacaaaag ccgcaggggc aatggcaccc gtccaacccc cggttccgcc gctggcagca
    12061 ccgcaaagct ccccagcagc gaaccgcccc acaggatcga caaacccgac agaagcaggc
    12121 cgcgcgcttt tgagatcatc ggttcctcat ctccacaggt agtcgccatg atagctgcga
    12181 gtcggcggcg gcggacagcc aaactggtct gccgcgagac gacgggcttg cagggtgcca
    12241 aaggctgtgc caaaagcaca aagtcatgga gcttgcgccg ctgtgtgtcc ccgtcgagat
    12301 tgccgtccga aacggtgccc tgcgctgttt tcggctggcg ctggccgtca gcgataccgg
    12361 tctgcggctg cgatcgccgc tgcctgatga gctgttgcgg gaaccgctgc ggattcgctt
    12421 gacgctgccc cccccgaccg aagagctggc gcgtctgttt gccgacgagt gggatggcaa
    12481 tctgatcgtt tttgcccgtg ccgccgcaga agtcgtcgcg cccaatacgc caaacgaaaa
    12541 acgactgtgg cgactgctgg tgtttgaaaa gcccgatccg gcgatgcgcg aaaaaatcca
    12601 gcgctatgtc gatttgcgaa ccgacgcggc gtagcgttta gtcgacgaca tccaccttgg
    12661 tgccaagcgg cacagcgtcg aacagtcgct gcgcaaagcg ctgcggaaaa cgcacgcagc
    12721 catgggaatc aggatgcccg acgagcggcc cggcgtgcag gccaatcccg ccgaaaatcc
    12781 gcataaagta cggcatcggt gtgccgcgaa acgtcgtccc cggcggcgga gtctggtcgt
    12841 ggttgtcgac attgctgagc aagacttcgc ccatttccgt cacgtagtcg ccgtaccgtc
    12901 cggacacata cagcggcact ttttcgagca cgctgtagct gcccttgggc gtcttgtggc
    12961 tctgccgacc gctgcaaatc gacgactgcg cgacgagctc tgcgccttcg aacaaatcgg
    13021 ccttctgtgt ctgcagatgg atcaccaagt gcggatgcaa gagatcgtcg tggaccgccc
    13081 agaaaaagtc gccgcctttg tcacgggacg aaaacttcac cgaaccgacc gaatcccgtc
    13141 cgtccgaaca gccgacaaag ccaagcacaa gacacgcgga accgagcaca gccccgaacc
    13201 ggcgacgcca aagcccaagc agcggcagca gcagtacgac gaagacagcg acgggagagc
    13261 tgggacgcgg acccatcgag caggccccga tcacaaacgg ctgaccgatt tcgctgcgct
    13321 caccaacgaa atagacgacg ggctgctgat tgagcaaaat tccgtcgacg cgcacttcga
    13381 ttttggcctg actgccgcgc ggtcccgtcg aaagcagcgt gcgttcataa cggccatgac
    13441 cttcgtcgcg caccgaatcg agccactcgc catggtcggc agaaatcatg accggaagcc
    13501 ccggcccgag cggctgaccc agcacatccc gtggcgtcac cgtcaccgtc accgtatcga
    13561 cgcccggcgc tgcgatgtca cggtttacgc ccacgtcgga atcgagcgcg ctcaaagaat
    13621 accgcggcgc attttgcaga cgggccagtg cgttttccag gcggatctgg ccaaagccat
    13681 ggcgcggccc gaacccctga aagtttggca gcagcgacgt ggttcgcaaa agttcgcgca
    13741 catcgtttgt caaaagtccc ggcgaaaact gaagcaaaag cgccactgct ccggcagcgt
    13801 gcggcgcagc ctgcgacgtt ccgcgcagca caccatgcag cccatcgtcg gcaatcagca
    13861 gaccggaatc gtggggcagc gcaaacgacg accgcggatc actttggatt gtgccgctcg
    13921 acagcgccga cacgataaac tcacccggcg ccagtacatc gggggcaaaa cgaccgtcgc
    13981 gcagcggtcc gcccgaagaa aacgtcgcca cccggtttag ctcgcgatca aaactggcca
    14041 acttgccgtc ggcgcgcgtc cacgaaagcc gcgaccgctg ggcaccgacg gaaatcaccg
    14101 accgagccga cgccggaatc gccaaatagc catccggatc caagtgcccg cgcagcgaca
    14161 catcgaggtc gtcgctcgaa aacactttcc acaggtcgta ccgtcgcgtc tgtccctgga
    14221 tttccaggtg ccagtcgccg gctttcggat agccttggcc cgcgggctta aaaatcgaca
    14281 caagcacgcc gcgcaggccg gtttccgcca catcgctcgc atcgattacc gcttcgcctt
    14341 cgtcgggaaa gctcgcctgt agcgactgac ccatcggcac ttcaaagcgg cggccacttg
    14401 gggaacgcag cgtcagcgtc agattcgccg ttccgtcgcg cagcggaggc gccgcagcgt
    14461 catagtacag ctccagcgag gtcgtacttt cctgcaccgg aatgtcggcg tggtcgatgt
    14521 gaatcgtaat gtcgtgtgtc ccatgaaaca gcgacgagct ggaatgaatg tcgaggtcgc
    14581 ccgagttgcc cgctgccgcg accaaaatcc gccccggacg ctgcgcaaac atttcgtcga
    14641 gcgccacttc gatgaggctg ctgccatcgt gcgagccacc gtctgaaccc aggctcaagt
    14701 tgacgaccag cggcagcttg tccgcgtcag cccgatccag gcaaaaacgc acccccagca
    14761 agatgtcggc gtcttcaaac gacacttcat cggcagtcgc cttggcgatg cacagcgtcg
    14821 attccggagc gatgccgacg taacgaccgg cggcaaactt ggccgtcgtc gcaagtccag
    14881 acgaagccgc gattccagca acgtgggtgc cgtgaccgtt gatgtccgtt tcgcgaatcg
    14941 caaacgcggg ctgcatgcct ttggcttccg cgtcgaagac ggcgtttatg tcctgtgcgg
    15001 tgacgatctg catgccgtcg taatcgggaa attcggggtg gcgtccgtcg cggcgctcgg
    15061 atcgactgat cagataggca atccgggtct ggccggcctt gtcgcgcaag tcggcgtgcc
    15121 gaaagtccac cccggtgtcg acgatgccca ccatcacgcc tttgccggtc aggccaaatt
    15181 tgtcggtcag cgcctgcccc ccaatcagcg cccgcgactg atcgagcagc ggcctccatc
    15241 ggtgcggccc gtcgatgcgt cgcacttgcg gattttctag cagcgtgcgc agcgcggatt
    15301 tgtcgacgga aacttgtgca atggtttggc tcagcggcgt cacgttcagc cccgccgccc
    15361 gcagcgactt cccaccgtcc gtcgtttgta ccaaaagccg cagccgcccc gcctcgtctt
    15421 gggacgcgga acccggcagc acacccagga aacgaaacat ctgggtcttg gccgccatag
    15481 acagccgtcc cgatccgagc cgcgagtatt catccggcaa aagaggcggt gccgagctgc
    15541 ccggcggcag aggcaccgac cgaaccaaag cgcggccaga ttcctgcgca tgcgacgggg
    15601 acaagcccag cccacacagc atggccagcg caaaaagaaa gttccgccaa gcggacatca
    15661 tggctagcag agtgccagag ccagccagcc cgctgtcaac gtttccaggc cgaaaccgcc
    15721 cgaaagtgac caaatttgac caaaacatgg ccaaactgcg gcgcttgctg ttgcgtccgc
    15781 gacgctcttt gggttatctg cgtgcgatgt cgatttatct ggtcacggga ggctgcggct
    15841 tcatcggcag caacctggtg cacgcgcttc tgcagcgcgg cgacgaagtg cgcattctcg
    15901 acaatctgag cagcggacgc ttttccaaca tcggcaagct gttggacagc caccctcggc
    15961 aggtttcgct gtggcggggc gatatcttgg acggaccgct gctcgatcgc gtgatgcacg
    16021 gcgtcgaata cgtcgcccat ttggcggcga tgccggcggt cacttggtcg gttgcccagc
    16081 cgatttggtg cgatcggata aattccggcg gaacgctgca agtgctcgac gcggccaggc
    16141 ggcaaggcaa ggtcaggcgg gtggtgattg cggcctcgtg tgcggcttac ggcgacctaa
    16201 acccgacgga accgaaacgc gaaagcgacg aagtggcccc gatgtcgccc tatgcggcgg
    16261 cgaagctcgc gagtgaacat cacggatcgg tctacagcca aatctacggt gttccgacgg
    16321 tgagcttgcg ttttttcaac gtctttgggc cgcgccaaga tccctcgtcg cagtacgcgg
    16381 cggtggtgcc caattttgtc cgcgccgcct tggaaaacaa gcgacccaag attttcggcg
    16441 acggcaaaca gtcacgcgac ttcgttttcg tcgacaacat cgtaagcgcc attcgcctgg
    16501 cgtggacctg cgatgaacgt cgcgtagccg gccaggtcat gaacatcggc tgcggcgaac
    16561 aggtgacgct gctcgacctt gtcgccgagc tagaaaagct gtgtggtcga tcgctcaacc
    16621 cggaattttt gccggcgcgc gaaggcgagg tccgtcactc gtttgcggac attgcacggg
    16681 cccgctctct cctcgggtat tcgccagagg ttcgcttccc cgaggggctt gcgcgtacac
    16741 tacgctggtt tgcgcaagag aaggacgccg catgaacagc atgaccggct ttggaggcgc
    16801 caagggagaa gtcctgggcc gccatttttc cgtcgaaatc cgcagcttca accaccgctt
    16861 tttggaagtg aagctgcgcc tgccgtctgg atttgcc
//
LOCUS       DAORGN010000101        16882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1380_length_16882_cov_6.410234, whole genome shotgun sequence.
ACCESSION   DAORGN010000101 DAORGN010000000
VERSION     DAORGN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16882
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_16882_cov_6.410234"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PKL17_12240"
     CDS             complement(<1..1325)
                     /locus_tag="PKL17_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK45549.1"
                     /translation="MGKDRRIRWLHLSDFHQGVNRLDQKTEVPELLRRLQSDLETLHR
                     DGPIDLVLFTGDLTQSGEAKQFESLGTFLDLLLGRLRELGSEAVLFAVPGNHDLVRPD
                     GSDMAAVVFDAWEKHPKVQEAFWDPDAKNYAQYHKPISTAFANYRSFAKSWLRKHPLP
                     SWIEVRAQGLLPGDFAATIDRNGAKLMIAGLNSAFLQLGDGYYKGRLELSPRQLGQVA
                     GPRWEETHDAALLLSHHPPDWLNPRSLVDYKSLMIQRERFAAHLYGHMHEPQVLQHSE
                     GGGSERFRIQAASLWSRDRYGKDAHETRLHGYSIGQIDLDDGLMRLWPREYVEKADGE
                     WRFEPDNRRYYLHGDGQLQRPIPVKRLRPPPVVVPVSGGSPGSDPAVRALASDPTLAR
                     DSGSDAGLTAISLEHYVPRERLERPLRRYLRGETHLRAGRRPGPRRAHH"
     gene            complement(1471..3579)
                     /locus_tag="PKL17_12245"
     CDS             complement(1471..3579)
                     /locus_tag="PKL17_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK45550.1"
                     /translation="MRKSNWLVGMCVAAIGLLRADLPSAQPVLLPHSVGQTEQARFLR
                     SFGLPPTDWSQPDFDDASWPRLVQAGERSASREPGAVLVPSLWTTELPPSTATAAPGF
                     PPASESAAPLASGPILDEPATPGNEPDVPVRDSATDVGVMSSPPVAVVDVAGAALRAG
                     LASTLPAAPLLASPTLGKRSGGSARDLAEPLLPACGGSLFVRRKFDFPGAPTAAGLSG
                     SIGTLLLRLRYSDGFVAYVNGAEVLRSRLPEPPPGDLPLFAMDRGPSDPERYYLPISA
                     LPLRAQGNVLAIEAHPKSPLRCPQIEAELLALSGPRLLRGPYIEKMSETTLDLTVETE
                     LPSRVLLRYGRGDKLSSRDREAESVGPLQNVHRLHIVGLRPGTLHHYQVALLGEGSRR
                     ADLPMQTFHTPPGAGMPLKVVVYGDSRSGHPVHAQVMQAILAEDPDLVLHTGDLVERG
                     TEEGDWDRFFAVTAPLLSRVPVYVSPGNHDYAIRKQGALRLFGLFATQFAPQNVQPKT
                     DTLLPSRVLATTVAPAAPLGDLSTGPRGYYSFDTSGIHFVCIDSNQAGKAEQHHWLEA
                     DLAQATAQIPKPRAIIAWLHEGPFSAGWHGDYPTAIKHIVPILTKYQTSLLVSGHDHD
                     YERGQRLGLDYVVTGGGGAELRPLRCDPRRRRCKNQPQFFANEHHYLKLDIQPGALRI
                     CPRRPDGTPLEECQVLRLRR"
     gene            3968..4144
                     /locus_tag="PKL17_12250"
     CDS             3968..4144
                     /locus_tag="PKL17_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45551.1"
                     /translation="MVQASLGERDRAIKILAKSIFRELKMNGYETRQIVALSSELLGL
                     VTTNMKPDGIDGGD"
     gene            4174..5220
                     /locus_tag="PKL17_12255"
     CDS             4174..5220
                     /locus_tag="PKL17_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45552.1"
                     /translation="MKPHKSAIPVACTLSLVLAAWPSSIRADGSGSRDAAQWPFAVDS
                     PWNLPLGSAAELESPDAACSKMLRDEKIGTDINAAQWSHPVYVAATTDPLVKITIKSK
                     VVATIPVPSGAEPAQPRTSDSDAHLHIVDPQHRYVHEMWHAKRRTTGISVEGYSRIDL
                     SSAGVGEGGERAYGGSAIGGLIRRPELASGIRHALAVALPRTHLKLGPVWPAVQQDNG
                     AETTYHGTVPMGQLVALSLHKTDLDTMGLSPAGKTLAQALIDYGAYVVDASSDLTLYA
                     EPSAEADIDPARADLIKLRPFLRCVKNASKTAVGGPGTRVAPLAAPLRAPLPAPTSLA
                     ATPRAKAPKEKALK"
     gene            complement(5189..7168)
                     /locus_tag="PKL17_12260"
     CDS             complement(5189..7168)
                     /locus_tag="PKL17_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45553.1"
                     /translation="MPSAQQHVILPQFGRTLGAQKTGADHAKMGAVIDPESRPQKVET
                     VRKLVQAGQFSPQLYAFLLDELLVCVDAKASDYREWLGFASLCAVRLDRPVCAALCTA
                     LASGPQAALSLLPEDGASRQRAYLLAAQAKSAGVDSARLVSEAAEQAQKAGLNVSAAL
                     FLGQVGKRSAAKELWLRLLGGSLPPYERALVHAQLALLFLRSDSASDAADGKRHAVLC
                     GQILDEIADDYESQNQRALALDCYRVLSQLGEASGAFENVTEGYLGMLRIFKSERLVG
                     EALWVYDELLRIATDAGEHELCAEQCRDAAAFLYRCGLPTSAQRYLLSAADALARVAD
                     VRLRAGAVRLAEHALLSAADLLSTLSAPDRLHRVLEQLAALRSDVAERSRYQRLAELI
                     GTAGAPARSDSGKPAAKSVETLPGSASQRAALPAVWSLDLLEWEANASPELVCLRLLL
                     DASRPELTRRHALLALLLLDGADPTQKSEAVQIRLVGSLGSLRAYEAIAPLARLYHTS
                     RATQPGGSALRVAILDVLPRLPFPRSLQLVVEALQDPALAVRSQAQASLARLGTVELL
                     GPLGQILVDASDLGVKLAIVSALGRIADPRAVERLLQVFLQSDDPLRREARRGLVSLR
                     DASLRPLYARALLSVSADRAQDLNILISELFPSGL"
     gene            7187..8254
                     /locus_tag="PKL17_12265"
     CDS             7187..8254
                     /locus_tag="PKL17_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45554.1"
                     /translation="MAAIDLLFDHKAQSERLHQVVLSALLLRSRLLERLLGIPGLRAT
                     DFEWEAEKRLFDLGFTVHDPNGNIEQVLVELKIDSELGEAQFQRQIQYVAQKPKARLV
                     YLILGLTQITVTEDRLGKWATTPGPAAAAQSPPFVYVDAARLGQWLSDPKPLLSDGEL
                     SQRREVRDLITAYREALDRLRARTTRFARRTVAQWESPDYYGFFAQCRDQIPSMKNAG
                     ISYEANPRGGVIVCNWKWTRLHPDAKLRIYLQMEESKLCLKLKVEDAHKAQRKKLWDA
                     AQAALRQIQSPIALKIEPTNYHSGTYMTFASLTDVLGQGELDIARVSQRLAEAEALVD
                     AMAPQLLLTAQKLVPVAPTPP"
     gene            complement(8272..10386)
                     /locus_tag="PKL17_12270"
     CDS             complement(8272..10386)
                     /locus_tag="PKL17_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK45555.1"
                     /translation="MWLCTSASVVGCNAVREVDTDADDPSDPVHPGDPTKIVDNALGC
                     RGKACSAAVPCASGQRCLEGVCVTAGESCENDSACQDDSRCFRGTCLPWDACKKLGSF
                     DPECKGAAFTPEQFKAPVIACKLNSFQSLSVPIVADLDKDGKPEVLTVANGVGIIAMS
                     GQDCKELWRKTISLQTSHQGNLAVADLDGDGFGEVVAVDAANRVIVLDYKGNLLATSP
                     TPTQEQNTTGQNWSAPAIADVDGVAPPEIIAGAQVSRFVKGSPSRIDVLWTKANKTPY
                     WGSLPIAADLDGDGRPEVITSDKVYDGLTGADKTPPALSSKPFYPAVADFNGDKKPDL
                     LLVMSDRGSQMVSVYDYTAQKTIFGPYRVAEGGWGGPAVIADFDGDKVPDFGLASASR
                     YYTYAFKCANNPKPADCTGTDPGVLWSKQTRDASSGGTGSSVFDFNGDGTAEVVYRDE
                     CWLRVYNGKDGKTLFAANITSNTCLELPVIADVDNDGHADIVVTSDSYGACGSTNPPE
                     PDTGTPWTGLTQGMFVLKDPMNRWMPSRPIWNQHAYHITNVNDDLSVPTREPDNWLSY
                     NNYRQNVQGGGAGTGSLPDPTGRTVPALDSTDCSKLWRLRGEVCNRGASPASAPLYAS
                     FYEGHPDRGGKLICTAQTTMPTAPGACQPVTCDWPNPPGRGVDLYLRVGDDGKGGRLG
                     GQCKNGNDLAHQPGATCSGIPG"
     gene            10661..14413
                     /locus_tag="PKL17_12275"
     CDS             10661..14413
                     /locus_tag="PKL17_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45556.1"
                     /translation="MITSPLGTSLTKRCLRCGMQVPVGEWHSCSAPEITISESKSDPS
                     LRDGSDADQDPVIGAILGERYLIESRLSQGGMGVVYKAKHVHLDTPLAVKILLQPQDP
                     IAQRRFLEEAKMASLVHHPNTVYIADFGLLSDGRTYLVMELLSGPTLAKVISQGAIEP
                     LRACQIAQGIATGLQAVHDHGIVHRDLKPENIFLVEQPGQKEFVKIVDFGIALRSNQS
                     LRQEQIEVLAAQAHKPPNDHSDHSGRLTEVGSVLGTPLYMSPEQACAGDIDSRADQYA
                     LGCILYEMLTGKVPFEGTTPTSLMFKHVTAMPPPLLRSNPKLKITNALETIVMRLLAK
                     QKESRYPSMKEVISELGKEIDLLLIQRGERVVLGKDAARLVQSGFRGLSKEAKQKVFL
                     HSVIATTTVLVLAGIGIYVGNRYVSSRNNPEVVKVTPQELADARLRATEFLVSELKDP
                     NPELRASVLSELGWTHDLSLGPKLTPFLVDENSEIAAQAAAALGELGDRLAIPKLLRI
                     SEQKVAANLHVAVAAALSQLGDSKGQKQLVESLDSSDPAVRQKAAYWLSEQGNPKAIE
                     IMLSLAQNPAISEEQAIRLLGRLVRGGDDQAKSLLRQKLNRPGRHEHKMLAAARLAVV
                     HDEEGTRFLREIAEQPGANQLFAARLMTSPENPVGIQLFRRVAADRSATPASRQIAME
                     GLALAGQSSDIRILLPILTTEPSLALRHQAAIAIVDIAAREPTALSDQSLTWARLASD
                     DDDFALRADAAGVLSEDKSTQAENLLLRLAKDKVASVRKSAIHALGKRTDAASLAALH
                     DGLSDEAAEVRAEALTALVKLSSLLVQLKQNDLVIQVRNWLEAVLRSQDMREQLLARI
                     GLQRLGDETQRAQLATLYQKVDAEQRRQIIDREDGDRELWKLALTDENWPLRFAAARR
                     LAQTSDRLSIPVLRQALSRNDASAGIAYGLLLRFGEQVVAPNLAAQLSTTVPIEQRIS
                     VVEGLAQLPPSVSVPLLLKAARDPSSAVRSQAAEVAAELPIVDGKPAGQPVLRLLSDD
                     GNAVIRARASALLLRLLKVPASSSALNVTTSGPKPGLPSDPEHSAVPAAGASSPVVTQ
                     NPASAPAPDKGTPIAELARRGRHALDTKDFAAAEQLLSKARRTCTRSAKTPGCAELSF
                     ELSVLLGRAHEAQGHTAEAMTEFELAMKLSPKVVGKADEKTAAQAAVVRLVPKLGIVM
                     VPKMNGKSCELLSLWMPPGSHQLTINGKPQTVSVQANQTVRAGSCP"
     gene            14452..15720
                     /locus_tag="PKL17_12280"
     CDS             14452..15720
                     /locus_tag="PKL17_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK45557.1"
                     /translation="MAYSLFAFLCAAQPLSAQETDGQSAALRNSGEVEILGARGVQVS
                     VDQKPVLTLPLSSPLSLSAGSHDITATQDGKTLQTTITVREGRALEVRFNFEVQAVSM
                     TVPPHALLLEKYEGIPSALADELAITTVRSLSKQRLAVLTPPKHAQIPNDCAQTIPCL
                     LSQAAVHESDYAVAVSVVQLAGAQPTSPIDYRIEGKIIDRSVSQESAQQTESCIGCSA
                     LQVTDKLRRMITELATKALSRTKGTLIITTEPANAAIKIDGVYRGQSPLRSVIWTGTV
                     SVSASLPSHRLQSERVTVREGAETPVQLTLQAEEQVAEQKRQVIILPRDVPLEREPRP
                     RWRLIAGSIGMGVGAVLMGFGVSALSIDGKCVSEPTGIARYCDNLYDTTAAGSSLVGV
                     GGAVLVGSGILLAWPGKPKQRPLSALQLQH"
     gene            15796..16458
                     /locus_tag="PKL17_12285"
     CDS             15796..16458
                     /locus_tag="PKL17_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNK45558.1"
                     /translation="MHHGTFVRLLLLSFTLFSSVSCDSLFGGLAKGNPESCVVTPSIC
                     NPDTEVCNTATRDCEPALRVESITPSAAPFDTEVNVTVTGKNFVPNMQLFVDGQPATS
                     LVLQSDTQLNSLFPASSRTKASVPIELVTPTTQRLRVDGMFHYFPWPQFAQPVNQPFD
                     GYQKFIRSGDFNGDGRPDLAVASDFTSGIGIFLTQADGNLIQAPSAVFSSRPFFVAVG
                     DA"
     gene            16611..>16882
                     /locus_tag="PKL17_12290"
     CDS             16611..>16882
                     /locus_tag="PKL17_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNK45559.1"
                     /translation="MRVSTDMQAAEGMSLDAQRSAIESYCAMMGIKLVKLCQDVMSGG
                     KDVRPGLADALRALQGSADVLIVLKFDRLSRSIKHFCELYETYFKDG"
BASE COUNT         3582 a         5184 c         4942 g         3174 t
ORIGIN      
        1 tggtggtgag cccggcgagg tccgggccgg cgcccggctc ggagatgcgt ttctccccgc
       61 agataacggc gcagcggacg ttccagccgt tcccgcggaa cgtagtgttc tagcgatatc
      121 gccgtaagcc ccgcgtccga tccactgtcc cgcgcaagcg tcggatcaga tgccaacgct
      181 cgcaccgccg gatcactgcc tggactgccc cccgataccg gaacgacgac gggtggtgga
      241 cgcagccgtt ttaccggaat cggacgctgt agctgcccat cgccgtgcag atagtagcga
      301 cgattgtcgg gctcgaaacg ccactcgcca tcggcttttt ccacatattc ccgcggccac
      361 aatcgcataa gcccatcgtc caggtcgatc tgtccaatgc tgtagccatg cagccgggtc
      421 tcgtgtgcat ctttgccata tcgatcgcgg ctccacagcg atgccgcttg aatgcgaaat
      481 cgttcgctac ctccgccttc actatgctga agcacttgcg gttcgtgcat atgcccataa
      541 aggtgcgcgg caaatcgctc tcgctgaatc atcagcgact tgtaatcgac caaactacgc
      601 ggattcagcc agtcgggcgg atggtgcgaa agcaaaagcg ctgcatcgtg tgtttcttcc
      661 catcgcggtc ccgcgacttg gccaagctgc cgtggactta gttccagtcg gcctttgtaa
      721 tatccgtcgc caagctgcaa aaaagcggaa tttaaccccg caatcatcag ctttgcgcca
      781 ttgcgatcga tcgtcgcggc aaagtccccc ggcagaagtc cctgcgcgcg gacttcgatc
      841 cacgacggca gcggatgttt gcgcagccat gacttggcaa acgatcgata gttcgcaaac
      901 gctgtcgaga tcggcttgtg atactgggcg tagttcttcg cgtcgggatc ccaaaatgct
      961 tcctgcacct tgggatgttt ttcccacgca tcgaacacca ccgcggccat gtccgatccg
     1021 tcggggcgca ccaagtcgtg attgcccggc accgcaaaca gcaccgcttc cgatcccagc
     1081 tcacgcagcc gcccgagcag cagatcgaga aatgtcccca gcgactcaaa ctgcttggct
     1141 tcgccgctct gcgtcagatc gccggtaaac agcaccagat cgatcggccc gtcgcggtgc
     1201 agcgtctcca ggtcgctctg taaccgccgc agcagctcgg gaacctcggt cttttgatcg
     1261 agccgattta cgccctgatg aaagtcggac aggtgcagcc agcgaatccg tcggtcctta
     1321 cccatcttgg cccccgagca gcaccggcgt tttccgttcg ctccgtgcgc gccgccctga
     1381 tactcaatct acgataactg gaaagccgcg aaaaatttgg gcacgcattc cctgcgttcc
     1441 tcctcggaag tgggcaaaat gttcacgcag ttatctgcgc agccgcagca cctgacattc
     1501 ttccagcggt gtgccgtcgg gccgtcgagg acaaatccgc agcgccccag gctgaatgtc
     1561 cagtttcaga tagtggtgtt cattggcgaa aaactgcggc tgatttttgc agcgtctccg
     1621 tcgaggatcg cagcgcaaag gacgcagctc ggccccaccg ccccccgtca ccacataatc
     1681 gagcccaagc cgctgtccgc gttcgtagtc gtggtcatgc cccgacacca gaagcgacgt
     1741 ctggtatttg gtcaaaatcg gcacaatgtg cttgatcgcc gtcgggtaat cgccatgcca
     1801 acccgccgaa aaggggcctt cgtgcagcca cgcgatgatc gcccgcggtt tcgggatctg
     1861 cgccgtcgct tgcgcgagat cggcttccag ccagtgatgc tgctcggctt tgcccgcctg
     1921 gttgctgtcg atgcagacga agtggatgcc ggacgtatcg aagctgtaat agccgcgcgg
     1981 cccggtgctt agatcgccaa gcggcgccgc aggtgccacc gtcgttgcca gaacccgcga
     2041 aggcaacagc gtatcggtct tgggctgcac attttggggc gcaaactggg tcgcaaacag
     2101 cccaaacagc cgcagtgccc cttgtttgcg gatcgcgtag tcgtgatttc ccggcgagac
     2161 atagaccggc acccgggaaa gcagcggcgc agtcaccgca aaaaaacgat cccagtcgcc
     2221 ttcctcggtg ccgcgctcga cgagatcccc cgtgtgcaag accagatccg gatcttcggc
     2281 caagatcgcc tgcatcacct gcgcatgcac cggatggccg gatcgactgt cgccatagac
     2341 cacgactttc agcggcattc ctgcgcccgg cggcgtatga aacgtctgca tcggcagatc
     2401 cgctcgtcgc gagccttcac caagcagcgc cacttgatag tgatgcagcg ttccgggtcg
     2461 cagtccgacg atgtgcagcc tgtgcacgtt ttgcaaaggt ccgacggact ccgcttcgcg
     2521 atcgcgactc gacagcttgt cgccacggcc atagcgcaga agcacccgcg acggcagctc
     2581 ggtttcaacg gtcaagtcga gcgtcgtctc gctcattttt tcgatgtacg gtcctcgcag
     2641 aagccgcggc cctgatagcg ccagaagctc tgcttcgatc tgcgggcagc gcagcggcga
     2701 cttgggatgc gcctcgatcg caaggacgtt gccttgggcc cgcagcggca gcgccgagat
     2761 cggaagatag taacgctccg ggtcagacgg cccgcgatcc atggcaaaca gcggcagatc
     2821 acccggcggc ggttccggca gcctgctgcg cagcacttcg gcaccattta cataggcgac
     2881 aaagccgtcg ctgtagcgca aacgcaaaag cagcgtgccg atgctgccgg aaaggcccgc
     2941 cgcggtcggt gcccccggaa aatcgaactt gcggcgcaca aacagactgc cgccgcacgc
     3001 cggcaaaagt ggctcggcga gatcgcgcgc cgatcccccc gatcgtttgc ccagcgtcgg
     3061 cgaagccaaa agcggtgcag caggcagcgt actggccaga ccggctcgca gcgctgcgcc
     3121 cgcaacgtcg acaaccgcaa ccggaggcga cgacatcacc ccaacatctg tcgcactgtc
     3181 gcgcaccggt acatcgggtt cattcccagg cgtcgccggt tcatccaaga tcggcccgct
     3241 agccagggga gccgccgatt cgctggcagg tggaaagccg ggcgccgcag tcgccgtcga
     3301 aggcggcaat tccgtcgtcc acagcgaggg aaccagcacc gcccccggtt cccgtgaagc
     3361 acttctttcg cccgcctgca caagccgcgg ccacgacgca tcgtcgaaat cgggctggct
     3421 ccagtccgtc ggtggcaagc caaagctgcg caggaatcgc gcctgttcgg tttggcccac
     3481 gctatgtggc aaaagaaccg gctgtgccga cggaagatcg gcgcgcagca gcccgatcgc
     3541 tgccacgcac attccgacca gccagttgct tttgcgcatg ctgggtccat tttggcaaaa
     3601 tccgcgcgat gctgtcggaa aagagggctc gcttgtcaga tcggcttggt atgtgtgaaa
     3661 agtgaaggcg tcgaggattc atccatcgtc gtgcgtcgcg cagtcaaagg ggcgtgtaat
     3721 ggatttcgca gtgcgcggga atgtgcgcag cactctgcgc aggcagatac gcgaaattca
     3781 ctcgatggtt gaaccataag caatcagcac caattcagca cagcgacgat aactcctggg
     3841 caaaagagga atcacgatgg cagacaaacc tggcgcgccc catgcaagag acagcgccca
     3901 aagtgaccca cggccccccg ccgaagtcgc cccaagttca tccgccacaa agtccaagga
     3961 gcagcagatg gtccaggcat cgctgggaga aagagaccgt gccatcaaga tcctcgcgaa
     4021 gtcgatcttc cgcgagctga agatgaacgg ctacgaaacg cggcaaatcg tcgcgctgtc
     4081 gtccgagcta ctcgggctcg tcacgaccaa catgaagcca gatggaatcg acggtggtga
     4141 ctagccacct gactttttgc caatctaccg cttgtgaagc cccacaaatc cgcaattccc
     4201 gtcgcctgca cgctatcact ggttcttgcc gcttggccca gttcgattcg tgcagatgga
     4261 agtggttccc gcgacgccgc ccagtggcca tttgccgttg attcgccgtg gaatctaccg
     4321 ctcggcagcg cggcggaatt ggaaagcccc gacgcggcct gcagcaagat gctgcgcgac
     4381 gaaaagatcg gtacggacat aaacgcagcg cagtggagcc accccgttta cgttgctgcg
     4441 acgaccgacc cactggtcaa gatcaccatc aaaagcaaag tcgtcgccac catccccgtt
     4501 ccttccggtg ccgagccggc gcagccgcgc acaagcgaca gcgacgcgca tcttcacatc
     4561 gttgatccgc agcaccgcta cgttcacgag atgtggcacg ccaagcgccg cacaactggc
     4621 atcagcgtcg aaggctattc gcggatcgac ttgtcaagcg caggcgtcgg cgaaggcggt
     4681 gaacgagcct acggcggcag cgcaatcggc ggactcattc gtcgccccga gcttgccagc
     4741 ggaattcgtc atgcgctcgc tgtggcactg ccgcgaaccc acttgaagct gggcccggtg
     4801 tggccggcgg tgcagcaaga caacggcgcc gagaccacgt accacggcac cgtcccgatg
     4861 ggtcagcttg tcgccctgtc gctacacaaa acggatctcg acaccatggg actgagccca
     4921 gccggaaaga cgctggcgca agccctgatc gattacggtg cgtatgtcgt cgatgcatcg
     4981 tcagacctga cgctgtacgc ggaaccgagc gcggaagccg acattgaccc cgcgcgtgcc
     5041 gaccttatca agctgcgtcc gtttcttcgc tgtgtgaaaa atgccagcaa gacggcagtc
     5101 ggcggaccgg gaacccgcgt cgctccactc gctgccccgc tgcgcgcgcc actgcctgcg
     5161 cccacgtcgc tcgcggcaac accccgcgct aaagccccga aggaaaaagc tctgaaataa
     5221 ggatgttcag atcctgcgcg cggtcggcgg acaccgacaa aagggctcgc gcatacagcg
     5281 gacgcagcga ggcatcgcgc agactgacaa gcccccgacg ggcctcgcgc cgcagcggat
     5341 cgtctgattg cagaaacact tgcagcagcc gctcgaccgc ccgcggatcg gcaatccgtc
     5401 ccagcgccga cacaatcgcc agcttgaccc ccaaatcgga cgcatcgacg aggatctgac
     5461 ccagcggccc cagcagttcg acggtcccaa gccgggccaa gcttgcttgt gcctgacttc
     5521 gcacggcaag tgccgggtct tgcagcgcct ccacgacaag ctggagcgag cgcggaaacg
     5581 gcagccgcgg cagcacatcc aggatagcga cccgcagcgc cgacccaccc ggctgcgtcg
     5641 cccgtgacgt gtggtaaagc cgcgcaagtg gtgcaatcgc ttcgtaagcg cgcagacttc
     5701 ccaagctgcc gaccaagcga atctggaccg cttcgctttt ttgcgtcgga tcggcgccgt
     5761 ccagcagcag aagcgccagc aatgcatggc ggcgtgtcag ttctggccga cttgcatcaa
     5821 gcaaaagtcg cagacagacc agttccggcg acgcatttgc ttcccattcc agcaagtcca
     5881 gcgaccagac cgccggtagc gcggcgcgct gtgacgcaga tccaggcagc gtttcgacgg
     5941 actttgccgc gggtttgccc gaatcggatc gggccggtgc gcccgctgtg ccgatcagct
     6001 cggccagccg ctggtagcgc gatctttcgg ccacatcgct gcgcagcgcc gcaagctgtt
     6061 ccagcacgcg atgcagacgg tccggcgcag aaagcgtcga caaaagatcc gccgccgaaa
     6121 gcagtgcatg ttccgcgagt cggaccgccc cggcacgcag acgaacatcg gcgacccgcg
     6181 caagagcgtc cgccgcgctg agcaaatagc gctgggcgga tgtgggcagg ccgcagcgat
     6241 acaaaaacgc cgcagcatcc cgacactgct cggcgcacag ctcgtgttcc cctgcatcgg
     6301 tcgcgatccg caacagctcg tcgtagaccc aaagcgcctc gccgaccaag cgctcgcttt
     6361 tgaaaatgcg gagcatcccc aaatagcctt cggtgacgtt ttcaaacgcg ccgctcgctt
     6421 cgccaagctg cgaaagcacc cggtagcagt cgagcgccag cgcccgctgg ttttggcttt
     6481 cgtagtcgtc ggcgatttcg tccagaatct gcccgcacag caccgcatga cgcttgccgt
     6541 ccgccgcgtc gctggcggaa tcactgcgca gaaacagaag cgcaagctgg gcatgcacaa
     6601 gcgcccgctc atacggggga agagaacccc caaggagtcg cagccacagc tctttggccg
     6661 ccgaccgctt gccgacctgc cccaaaaaca gcgccgccga tacgttgaga ccagcctttt
     6721 gcgcctgttc cgccgcttcg cttacgagcc gcgccgaatc gacgcccgcc gatttcgcct
     6781 gcgccgccaa aagatacgcc cgctgccggg acgcaccatc ttccggaagc agggacagcg
     6841 ccgcttgcgg tccgctcgcg agtgccgtac acagcgccgc gcaaacgggc cgatcgagcc
     6901 gcaccgcaca caagctggca aagccgagcc actcgcggta atcgctcgct ttggcatcaa
     6961 cacagacgag cagttcatcc agcagaaatg cataaagctg cggcgaaaac tgcccggctt
     7021 gcacgagctt tcgcacggtt tctacttttt gcggtcgcga ctcgggatcg atcaccgcgc
     7081 ccatttttgc atggtctgcc cccgttttct gcgcccccag cgtgcggcca aactgcggta
     7141 agatcacatg ttgctgcgcc gaaggcatgc gcagctgggg gaagacatgg cggccattga
     7201 tctgctgttt gaccacaaag cccaaagtga gcggctgcac caagtggttc tgtcggcgct
     7261 gcttctgcgc agtcggctgc tggaacggct gcttggcata ccggggctgc gggcaacgga
     7321 ctttgaatgg gaagcggaaa aacggctgtt tgacctcggc ttcacggtgc acgatccaaa
     7381 cggcaacatc gagcaggtgc ttgtcgagct aaagattgat tccgagctgg gcgaagcaca
     7441 gtttcagcgt cagattcaat acgtcgcgca aaaacccaaa gcgcggcttg tttacctgat
     7501 cttgggactt acgcagatta cggtgacaga agataggctc ggcaaatggg caacgacgcc
     7561 cggtccagcc gctgctgcac aaagtccgcc ctttgtgtat gtcgatgcgg cgcggcttgg
     7621 gcagtggctt tctgacccca aaccgctgct ttccgacggc gagctttcgc agcgtcgcga
     7681 agttcgcgat ctgatcaccg cgtatcggga agccctggac cgcttgcggg ctcgcacgac
     7741 ccgatttgcc agacgcaccg ttgcacagtg ggaatcgccc gactactacg gcttttttgc
     7801 ccagtgtcgc gaccaaattc cgtcgatgaa aaacgccggg atcagctacg aagccaatcc
     7861 acgcggcgga gtgatcgtct gcaactggaa atggacccgc ctgcatcccg acgccaagct
     7921 gcgcatctat ctgcaaatgg aagagtcgaa gctgtgtctg aagctgaaag tggaggatgc
     7981 gcacaaagca cagcgcaaga agctgtggga tgcagcccaa gccgcgctgc gtcagatcca
     8041 aagcccgatc gcgctcaaga tcgagccgac caactaccac agcggcacgt acatgacgtt
     8101 tgctagcctg accgacgtac tgggacaagg ggaactggac atcgcgcggg tcagtcagcg
     8161 gcttgccgaa gccgaagcgc tggtcgacgc gatggccccg cagcttctat tgaccgccca
     8221 aaagctggtg ccagtcgcgc ccacgccgcc gtaatggatt cgcccaatca gctacccagg
     8281 aatgccgctg catgttgcgc cgggctgatg cgcgaggtcg tttccgtttt tgcactgacc
     8341 cccaagcctg ccgcctttgc cgtcatcacc gacgcgcaag tacagatcaa cgccgcgacc
     8401 tgggggattt ggccagtcgc aggtcacggg ctggcaggca ccgggagcgg ttggcatcgt
     8461 cgtctgcgcg gtgcagatca gcttgccgcc acggtcggga tggccttcat agaaactggc
     8521 gtagaggggt gccgatgccg gggatgctcc gcggttgcag acctctccgc gcagacgcca
     8581 cagcttgctg caatcggtcg aatcgagcgc cggcaccgtc cgccccgtcg gatcaggaag
     8641 tgatccggtt cccgcgccgc cgccttggac gttctgtcgg tagttgttgt agctcagcca
     8701 gttgtccggt tctcgcgtcg gcaccgagag atcgtcgttc acattggtga tgtgataggc
     8761 gtgctgattc cagatgggcc gcgacggcat ccagcggttc atcgggtctt ttaggacaaa
     8821 catcccttgg gtcagcccgg tccacggcgt gccggtatcg ggttcaggag ggtttgtgct
     8881 gccgcaagcg ccatacgaat cgctggtgac gacgatgtcg gcgtgcccgt cgttgtcgac
     8941 gtcggcgatg acgggcagtt ccaggcaggt gttgctggtg atgttggctg caaacagcgt
     9001 tttgccgtct ttgccgttgt agacgcgcag ccaacactcg tcgcggtata cgacttcggc
     9061 agtgccatcg ccgttgaagt caaacaccga tgaccctgtg ccgcccgacg acgcatcgcg
     9121 ggtctgtttg gaccacaaca cgcccggatc cgtccccgtg cagtccgccg gtttgggatt
     9181 gtttgcgcac ttgaaggcat aggtgtagta gcggctcgcc gacgccagtc cgaaatcggg
     9241 gactttgtcg ccgtcgaaat cagcaatgac tgcgggtccg ccccacccgc cttccgcgac
     9301 gcgataggga ccaaagatgg tcttttgcgc ggtgtaatcg tagacgctca ccatctggct
     9361 gccgcgatcg gacatcacca gcagcaagtc gggttttttg tcgccgttga agtccgcgac
     9421 ggcaggataa aacggcttgc tcgaaagggc cggcggggtt ttgtcagcgc ccgtcaagcc
     9481 gtcatacacc ttgtcgctgg tgatcacttc tgggcgacca tcaccgtcga ggtccgcggc
     9541 aattggcaaa gacccccaat acggtgtctt gttcgctttg gtccaaagca catcgatacg
     9601 cgacggcgaa cctttgacaa aacggctgac ctgggcgcct gcaatgattt caggtggggc
     9661 gactccatct acatctgcaa tcgccggagc gctccagttt tgccccgttg tgttttgttc
     9721 ctgcgtcggc gtcggcgatg tcgccagcaa gttgcctttg tagtcgagca caatgacgcg
     9781 gtttgccgca tcgaccgcca caacttcccc aaagccatcg ccatccagat cagcgaccgc
     9841 gagattgccc tggtgcgagg tctgaagcga gatcgtcttg cgccacagct ctttgcagtc
     9901 ttggccgctc atcgcgatga tgccgacgcc gttcgcgacc gtcagcactt cgggttttcc
     9961 gtctttgtcg aggtcggcca cgattggcac cgacagcgac tgaaaactgt tcagcttgca
    10021 agcgatgacg ggggccttga actgttcggg ggtaaaagcc gcgcctttgc attcggggtc
    10081 aaatgaaccg agctttttgc acgcatccca cggcaagcaa gtgccccgga agcagcgcga
    10141 gtcgtcttgg cacgcgctgt cattttcaca actttcgccc gcagtcacgc agacgccttc
    10201 cagacagcgc tgcccgcttg cacacggaac tgccgccgag caggctttgc cacgacaacc
    10261 cagcgcgttg tcgacgatct tggtcggatc gccaggatga accggatcgc tcggatcgtc
    10321 ggcatcggtg tcgacttcgc gcaccgcgtt acaaccgacc accgacgcgc tggtgcaaag
    10381 ccacaagaca gcgatcgccg accgcgttct ttgcccagaa gatcgaagtt tccgtcgaga
    10441 gcgctccgat tccatgcttc gcaacgggtc catggcatac ctccagattg gagccgcgaa
    10501 cataccatcg cgatgtaggt tgttggtcaa agcgcagcca ctcgacccga cgaatcccgc
    10561 gctggcacga ccatttagcc aatcaacagc caggtggcga accgacggca aattcagatg
    10621 aattggctga tgtataattg gcccttcggg ggaagctttc gtgatcacat cgcctcttgg
    10681 tacgtcactc accaagcgct gtctgcgctg cgggatgcaa gttcccgtcg gtgagtggca
    10741 tagctgtagc gcgccagaga tcaccatcag cgaaagcaag agcgacccgt ctttgcgcga
    10801 cggcagtgac gccgatcaag atccggtgat cggtgccatt ctcggcgagc gatatctcat
    10861 tgagtcgagg ctcagccaag gtggcatggg cgtggtctac aaagccaagc acgttcacct
    10921 ggacacgccg ctggcggtaa aaattctgct gcagccgcag gacccgatcg cccagcgccg
    10981 attcctcgaa gaagccaaga tggcgtcgct ggtgcatcac ccaaacacgg tctacattgc
    11041 cgactttggg ctgttgtccg atggacgcac ctatctggtc atggagctgc tcagcggtcc
    11101 gacgctggcg aaagtcattt cgcagggcgc aatcgaaccg ctgcgcgcct gccaaattgc
    11161 acaaggaatc gccaccggac tccaagccgt ccacgatcac ggaattgttc atcgggattt
    11221 gaagccggag aacatttttc tcgtcgagca gccgggtcaa aaagagtttg tgaaaatcgt
    11281 cgactttggt atcgcgctgc gttccaacca aagtcttcgc caagaacaaa tcgaagtctt
    11341 ggcagcgcaa gcgcacaagc cgccaaatga ccacagcgat cacagcgggc ggctcaccga
    11401 agtgggatcg gtgctaggaa cgccgctcta tatgtcgccg gaacaagcct gcgccgggga
    11461 cattgattca cgcgcggacc aatatgcgct gggctgcatt ttgtatgaaa tgctgaccgg
    11521 caaggttccg tttgagggga caacgccgac gagcttgatg tttaagcacg tcaccgcaat
    11581 gccacctccc cttctgcgca gcaatcccaa actgaagatc acaaacgcgc tggaaacgat
    11641 tgtgatgcgg ctcctcgcca agcaaaagga gtctcgctat ccgtcgatga aggaagtcat
    11701 ctcagagctt ggcaaagaga tcgatctgct cctgattcag cgcggagaga gagttgtctt
    11761 aggaaaagat gcggcccgct tggttcagag tggattccgc ggtctgtcaa aggaagcgaa
    11821 acagaaagtc ttccttcatt cggtcatcgc gacgacgacc gttctggttt tggcaggaat
    11881 cggaatttac gttgggaatc gatacgtatc cagccgcaac aatccggaag tcgtgaaggt
    11941 cacgccgcaa gaactggcag acgcccggct gcgcgctaca gagttcctgg taagtgagct
    12001 aaaagatccg aatccggagc tgcgtgcttc ggtgctgagc gagctgggat ggacccatga
    12061 tctaagtctg ggtccgaaac ttacgccgtt tctggtagat gaaaacagcg agatcgctgc
    12121 gcaagccgca gccgcgctgg gtgagctagg agaccggcta gcgattccca aactactgcg
    12181 gatcagtgaa cagaaagtcg ctgccaactt gcatgttgcc gtcgcagcag cgctttccca
    12241 actgggtgat tcaaaagggc agaaacagct cgtcgaatcg ctggatagtt cggaccccgc
    12301 agttcgccaa aaggccgcgt actggctgag cgaacaaggg aatcccaaag cgatcgagat
    12361 catgctgagc ctggcgcaaa acccggcgat atccgaagaa caagcgatcc gtctgctggg
    12421 tcggctggtg cgaggtggag acgaccaagc caagagtctg ctgcggcaaa aattgaatcg
    12481 accgggacgt catgaacaca aaatgctcgc tgcggcgcgg ctggccgttg tccatgatga
    12541 ggaaggaacc cgattcctgc gtgaaattgc cgaacagcca ggcgccaatc agctctttgc
    12601 agcccggctg atgacatccc cggaaaatcc ggtaggaata cagcttttcc gtcgagttgc
    12661 tgccgaccgt agtgcgacgc cagcgtcacg ccaaatcgcg atggaaggtc tggcactggc
    12721 tggacaaagc agcgacattc ggattctgct gccgattctg acgaccgaac catcgcttgc
    12781 gctgcgtcat caagcggcga ttgcgattgt agacatcgcg gcgagagaac caaccgcgct
    12841 gtctgatcag agtctgactt gggcgcgact tgccagtgat gatgatgatt ttgcactgcg
    12901 cgcggacgcg gcgggagtgc tcagcgaaga caagtcaacg caggcagaaa acctgctgtt
    12961 gcgattggca aaagacaaag tcgcaagcgt tcgaaaaagt gcgattcatg cgctaggaaa
    13021 acgcacagac gcagcgtcgc tggcagcgct gcatgatggc ctgtccgatg aagccgcgga
    13081 agtgcgagcc gaagcactca cggctcttgt aaagctcagc tctctgcttg tgcagctcaa
    13141 acaaaacgat ctggtcatac aagttcggaa ctggctggaa gcggtgctgc gtagccaaga
    13201 catgcgcgaa caactgctcg cacgaattgg tcttcagcga ctgggagacg aaacccagag
    13261 agcgcagctt gccacgctgt atcaaaaagt agacgcagag cagcgtcgtc aaatcatcga
    13321 tcgcgaagat ggggacagag agctgtggaa actggccctg accgatgaaa actggccgct
    13381 tcgattcgca gcggcgcgac gcctcgcaca gacaagtgat cggctctcga ttcccgttct
    13441 ccggcaggcg ctcagccgga atgatgcgag cgcaggaatc gcttacggac tccttcttcg
    13501 ttttggcgaa caagttgtag cgccgaactt ggcggcacaa ctttcaacga cagtaccgat
    13561 tgagcagcga atttccgtcg tagaaggact cgcacagctc cccccttcgg tcagcgttcc
    13621 tctcttgtta aaagcagcgc gcgatccatc cagcgctgtc cgatcacaag cggcagaagt
    13681 ggcagcagag cttcccatcg ttgatgggaa acctgcagga cagccggttt tgcggcttct
    13741 gtccgatgat gggaatgcgg tgattcgcgc acgcgcgagc gcacttcttt tgcgacttct
    13801 caaagtccca gcgtcgagca gcgcactgaa tgtgacaaca tctggaccca agcctggtct
    13861 tccgtccgat ccggagcata gcgcggttcc tgctgctggc gcgtcatcac cggttgtcac
    13921 acagaacccc gcttcggccc ctgctccgga caagggaacg ccgattgcag agcttgcaag
    13981 acgcggtcgt cacgcgcttg acaccaagga ttttgccgca gcggaacagc ttcttagcaa
    14041 agcgcgccgg acatgtacgc ggtcggccaa gacaccgggt tgtgcagagc tttcgtttga
    14101 gctgtcggtt ctgcttggtc gcgcacacga agcacaggga cacaccgcag aagcaatgac
    14161 agagttcgag cttgcaatga agctgagtcc gaaagtcgtc gggaaagcgg atgaaaaaac
    14221 cgccgcgcaa gctgcggtgg tacgactggt tcccaagctc ggaatcgtga tggtacccaa
    14281 gatgaacgga aaatcatgcg agctgctgtc gctgtggatg ccacccggca gtcatcagct
    14341 caccatcaat gggaagccac agacggtctc cgttcaagca aatcaaacgg tccgcgcagg
    14401 aagctgtcca tgatgtctcc ggtatctcgc ccacatcgcg cagccgtcgc ggtggcgtac
    14461 tcccttttcg cattcctttg tgcagcacag ccgctgtctg cgcaggaaac agatggacag
    14521 agcgcagccc tccgcaacag tggcgaagta gagattttgg gagcgcgcgg tgttcaagtt
    14581 tccgtggatc agaagccagt cctcacgctg cccctctctt ctcctctgtc actatcagca
    14641 ggcagccatg acatcaccgc aacacaggat gggaagacac ttcagacgac aatcacggtg
    14701 cgagaggggc gagccttgga agttcgtttc aacttcgagg tgcaagcggt cagcatgacg
    14761 gttccgcccc atgcgctgct gctggaaaag tacgaaggga tcccctcagc gctcgcggat
    14821 gagctggcga ttacgactgt gcgctcgctt tcgaaacagc gactggccgt actaacgccc
    14881 ccgaaacatg cacagattcc caatgactgt gcgcagacga ttccatgtct gctttcgcaa
    14941 gccgcagtcc acgagagtga ctatgcggtc gcggtgtctg ttgtccagct tgcaggcgca
    15001 cagccgacca gtccgatcga ctaccgaatc gaagggaaga tcatcgatcg cagcgtgtca
    15061 caggagtccg cacagcagac tgaatcctgc atcggctgta gcgcgctaca ggtgacagac
    15121 aagctgcggc gcatgatcac ggagcttgca accaaagcgc tttcacgaac caaaggcacg
    15181 ctgatcatta cgacagagcc agccaatgca gcgatcaaga ttgatggtgt ttaccgagga
    15241 caatctcctc tgcgcagcgt gatctggaca ggaacggtca gcgtatctgc cagcttgcca
    15301 agtcacagac tgcagagcga gcgtgtgact gtccgcgaag gtgccgagac cccggtgcag
    15361 cttacgctac aggcagaaga acaagtagca gagcagaagc ggcaggtgat catccttccg
    15421 cgtgacgttc cgttagaacg cgagccacgt ccgcgctggc ggctcattgc gggcagcatc
    15481 ggcatgggcg taggcgcagt tctgatggga tttggtgttt cagcgctttc aatcgatggg
    15541 aagtgcgtgt cagaaccgac aggaatcgcg cggtactgcg acaatctgta cgacacaacg
    15601 gctgcgggat cgagtctggt cggagtgggc ggtgctgtac tggttggcag tggaatcctg
    15661 ctggcctggc cgggaaagcc caagcagaga ccgctcagcg cccttcagtt gcaacactag
    15721 aaaagccatc ttcgtccacg accaaggggg ctcgaatggc actcctttca catcgcacat
    15781 gaacaggaaa catccatgca ccacggaaca tttgtccgct tgcttcttct gtcattcact
    15841 ctgttttcgt ctgtgagctg cgactccctc tttggcggac tggcgaaagg gaatcctgaa
    15901 agttgtgttg tgacaccatc catctgcaat ccagacaccg aggtctgcaa caccgcaacc
    15961 agagactgtg aacccgcact gcgcgtcgag tccatcacac cttcggcggc tccgtttgac
    16021 acagaagtca acgtgacggt gaccgggaaa aacttcgtgc ccaacatgca gctatttgtc
    16081 gatggacaac cggccacttc gctggtgctc caatcggaca cacagcttaa ttcgcttttt
    16141 cccgcaagca gtcgcaccaa agcatccgtt cccatcgagc ttgtcacgcc caccacccag
    16201 agactgcgcg tggatggcat gtttcactac tttccgtggc cgcagtttgc gcagccagtc
    16261 aatcaaccgt tcgatggata tcagaagttc attcgctcag gcgatttcaa tggcgatggg
    16321 cgtcccgatc ttgcggtggc ttctgacttc acttctggga taggaatttt cctcacacaa
    16381 gccgacggaa acctcatcca agccccatcc gccgtcttct cctcccgccc attctttgtt
    16441 gcggtagggg acgcataatc caattatgtc ttaactccta atcgcccaca tgtgggacgg
    16501 aaggagtggt catgaggagt gcgaaagggg tccacgtagg aatcggaaac agagactccc
    16561 agggacatta cggagggaat gagcagagtc cgcgcagagc ggtaggatac gtgcgggttt
    16621 ccacggacat gcaagcggcg gaaggaatgt ctctggatgc gcagcggagt gccattgaga
    16681 gctactgcgc gatgatggga atcaagctgg tgaagctgtg tcaggacgtg atgtcgggag
    16741 ggaaggacgt tcggccaggg ctcgccgacg cactgcgggc tctgcaaggc tctgcggatg
    16801 tcttgatcgt gctgaagttt gatcgactat cgcgttcgat caagcacttc tgcgagctgt
    16861 atgaaacgta cttcaaagac gg
//
LOCUS       DAORGN010000102        16872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1381_length_16872_cov_5.584349, whole genome shotgun sequence.
ACCESSION   DAORGN010000102 DAORGN010000000
VERSION     DAORGN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16872
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1381_length_16872_cov_5.584349"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKL17_12295"
     CDS             complement(<1..426)
                     /locus_tag="PKL17_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK45560.1"
                     /translation="MALLAIVHPQTASAQVAPRITIDSNPPGATIFVDGALQSERTGP
                     ATKVRINKGQHKLRLELEGYRPFEQTVTIGAATKFAFQLDKAPARLDIKYPATNDAAR
                     GAEIFVDGQPAGTVPQLVDLPAGRHLVEVRKPGMKVYTEN"
     gene            complement(634..2451)
                     /locus_tag="PKL17_12300"
     CDS             complement(634..2451)
                     /locus_tag="PKL17_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNK45561.1"
                     /translation="MRIHALPIFIAAICILAIAYRFYSAFIAAKVFALDGKNQTPAHA
                     RRDGKDYIPTSRWVLFGHHFAAITGAGPLIGPVLAAQFGFLPGLLWILVGVTLAGAVH
                     DFVTLSASVRSGGKSLAAMARQELGSRAGSVATLAIFFTVTVAISAMSFAVVKILEGS
                     PWATFTIGMTIPIALLMGSIMRGESHGLRLRAASLLGVGLLLFAVVYGDTIAHSRLAP
                     YLSLSFGTLVACVAAYGLLASVLPVWLLLVPRDYLSSYMKLGTLSVLIVAIMVVNPEL
                     KLEAFTQFVYGGGPIIKGKVFPFVFITVACGAISGFHSLIASGTTPKLIDQETDCRPI
                     GYGAMLLEGLVGITSLVAAACLHPADYFAINVPEAAFQKLGMTPVEIDLMSQLVGEKL
                     RGRTGGSVSLAAGIAQIFAQLPGARTLLGYFYHFIVMFEAVFILTTVDAGTRVARFLM
                     QDILGRVDDRFSRHDFVPGVWLASILVVMFWGGFLYTGTISTLWPLLGISNQLLATTA
                     LAVGTAVILRLHPTRRHYALVTAIPMLAVGATTLTAGWQSIWNNFSRDPSRVKAIVNS
                     GCTAILMGSCLLVLFTLGGRALSQNSAERASSSEDDKPT"
     gene            complement(2475..3902)
                     /locus_tag="PKL17_12305"
     CDS             complement(2475..3902)
                     /locus_tag="PKL17_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNK45562.1"
                     /translation="MSVFFSDDRGAVLFACDKSRVGIWLARKTGYRAGMESRLRSGWE
                     NDISMGVRAAVPRFFSTGAVGCIAGLSLCLWGCASSDVPLDDDKTGGDHRTAAGSDLG
                     SGGDLADDGSDLNVVPGDTVLRVHYPRNDARYSKLSIRGSGSGLSWTSGKAMTKVRDG
                     LYQARLKGVSSTLEWKPLLDDATWSRGKNFVVEPGQTVDIYPHFVTSKGRYLRVTGVR
                     STVLGNTRAAWLYLPPSFDENPEARYPILCMHDGQNLFDPRYAFGGRTWQVSQTLDAG
                     IDALDPSDSLPEVVVIGPENTDRRLYEYTPTKSSDPTYRDSGGGDQYLKFVVEELLPL
                     MNGLTSPVVLSGRLVSDPAKTTLAGSSLGGLITAYAGVKKPDVFGRIGIFSPSTWWDG
                     RYILSAVKASVGKPSRVYVDSGDGGGEGGDDWMNTAELAKTYRDIGFRDGANLQYVLE
                     PMATHNEDAWARRLPAALRFLLAGL"
     gene            4014..6923
                     /gene="uvrA"
                     /locus_tag="PKL17_12310"
     CDS             4014..6923
                     /gene="uvrA"
                     /locus_tag="PKL17_12310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNK45563.1"
                     /translation="MDKIVIKGAREHNLKNIDVELPRDKLVVLTGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFLEQMGKPDLDSIDGLSPAISIEQKSTSKNPRSTVGTIT
                     EIYDYLRLLYARIGTVHCHSCNLPIAAQTVQQMVDRVLRLPQGTRFSVLAPIVRSRKG
                     EFKKELDSLRRQGLRRVSVDGELFDLSEDTIKIDKNKAHTIEVYVDRLVVKSDVRQRL
                     TEAVELSLQLAEGIVKVSPVMLGDDALPDGVGITDENGDIIFSERFACINCGVSYPEI
                     TPRLFSFNNPSGACPACDGIGAKMFFDPELIVPNTDLALREGAIEPWEKRNGPFFQQI
                     IEALAQHYGFDMAVPWSDLPQNVRDIVLYGSKGDEVEFWFEKNGRRHTYKKEFEGVIA
                     NLERRLSEYERRRREEGTAPAGSDSFEAAYEEFSPYMNKSPCEECQGTRLRKEARFVR
                     VGNLPIHELTAKPIRDAHRFFTELRLSQRQAEIAERILKEVRDRLGFLVNVGLDYLTL
                     DRTAATLSGGEGQRIRLASQIGSSLVGVLYVLDEPSIGLHQRDNQRLLETLFRLRDMG
                     NTVIVVEHDEDTIRAADHIIDMGPRAGVLGGRVVAAGRVDEIMSSTASLTGAYLSGRK
                     KIEVPRSRRQSQNRYLVLKNGKEHNLRDVTVRFPVGVFTCVTGVSGSGKSTLVIDTLL
                     AALKQKLNHSKCQVGQHEGIEGLHHVDRVIDIDQLPIGRTPRSNPATYTGVFTHIRDL
                     FAALPDSKSRGYKAGRYSFNVKGGRCESCQGDGILRIEMHFLPDVYVVCEECGGRRYN
                     RETLEVKYKGASIADVLDMTVTQACEFLGHIPKVRQKLETLRDVGLGYIALGQAATTL
                     SGGEAQRIKLSKELAKKGSGRTVYILDEPTTGLHFEDIKQLLEVLNRLVDSGNTVIVI
                     EHNLDVIKTADYVVDMGPEGGEGGGSVVACGTPEDIARVPSSYTGQYLKKVLALS"
     gene            6933..7562
                     /locus_tag="PKL17_12315"
     CDS             6933..7562
                     /locus_tag="PKL17_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNK45564.1"
                     /translation="MKILSVTQLPIPDVKVVRFARFCDERGYFSEHFRTTDIAAHPDL
                     AHFAAQPIVQCNEAFSRRGTLRGLHFQWQPKMGKLVRPLCGRLIDFALDLRLSSPWFG
                     KIIGYDMPAATDRDFAEWIWVPPGFAHGTLLPEETVIEYFCTAAWNPNAEAGISPLAD
                     DLDWSLCDQQVAKLYADAVKNGPLLSEKDRVAPSLTAWKDDPRAGLITD"
     gene            complement(7580..8821)
                     /locus_tag="PKL17_12320"
     CDS             complement(7580..8821)
                     /locus_tag="PKL17_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45565.1"
                     /translation="MSVDFASGMRRCVLAGCLSLLAHLLPAILLSSLSLLEKLLPPPP
                     ITWDIVPAKRKTPPPLIAPEAVAAKPLVPDPPEPAQKQQLSRRGEPGGKIGKPTPPKT
                     PPLASLHGVGAASIEQDVGLRLVLRMAEVARSPHRQSVAGLLGAFPDSRLLVAGSALS
                     SGDELAQELFSLSDVLVIATTDPTGYRRSATALLAMGKKLRPLFDKLTARRVPTWDDR
                     ELLFDAPDLLAFLPRPEPLPSPPSDGQNPDASVGPEPPSLTAQLPALRRALPKQGPPL
                     VVEIFNVQGRLRLRGGIPTPRHIQLALSSDMDPQVHGRLVVSSAEEATQLRAAILQVG
                     DKLRSDWRFKLMGISTVLDHLQIEVKASELLVEGFVTGAALRSLLGVATTALQVLPSG
                     APPPPPDFSLPPAVDAGVDAQ"
     gene            complement(8903..9973)
                     /locus_tag="PKL17_12325"
     CDS             complement(8903..9973)
                     /locus_tag="PKL17_12325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK45566.1"
                     /translation="MLLALSSLLGAPGGIPSFNRLLIRAASEFAQVRRLPLHIVALTD
                     EPGADPDFLAGLPRAVSPTYVACRGNRRLFAAEVLGFVGRESAVVLGHVNLSPLGLGF
                     SRFGVIAHGTDVWSPLPILRRWSLQRSTVAAGVSEDTLRKLCAVQGVRPDRCQRLVNA
                     LDEQSLALAAATPKTACESSLRLRIVAITRLWIGEPKGIDLVIRSLPQLPDVDFEVVG
                     DGAARPQLVDLAQSLGVADRVHFVGRLSETEKHAAIARSHALVLPSSGEGFGIVYLEA
                     MAHRRPCLAASVGGAPEVVLDGETGVVVEPTVQAVCAGILRLRDPSLRQRLGDAGYQR
                     LYDHFTYAAFARHAEPFFARLY"
     gene            complement(9977..12019)
                     /locus_tag="PKL17_12330"
     CDS             complement(9977..12019)
                     /locus_tag="PKL17_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNK45567.1"
                     /translation="MAAVEGTGEGPPAGTLPEKILICLLSLAIVFLPVLSSLRSLADM
                     LRESHRLKWLTHSLGFVLHKLVPSDLSWVSLYVQNGTVWIGFLGALLAVAGGKHLGLS
                     TTGFLSKGRMRSAAEGFTSTVFVLVALVLTYASARMVAVERVTGENDLLPGGLHKWYV
                     QIIIPVVFALMGPRAAWRALDPNAGGGHGAPSPNEPHHDMSPRRRKLRWLVCGVTLIL
                     AIVMFVIGFWHDGSPKLQALVAKTEKLVGLVKWLGLFSMGTGFLLGLPVFVVMAGLAM
                     TLFFLAGTPIASLPNETLRLVANPQLPAIPLLTIAGYVLASGRSSHRLVRAYKSVLGW
                     IPGGMAIMVVIVCAVFTTFTGASGVTILALGGLVYPMLVRDGYPKGFSLGMVTAAGSL
                     GLLFPPSLPVILYSVAASAPGSVAPVSVDQLFIAGFLPGLCLVVLVSLYGMQQGYKAN
                     VPRQPLVWKEACEAVWAAKWDIVVPVVVMGSFLSGIATIVESAAIGAAYALIIELLVF
                     RNVHPIKDLPSAFLHAASLVGAVVVLLGVALGLTNYLVEAEVPTKLVEWVQAHIHSRW
                     VFLLILNVGLLVLGSVLEIYSAIVVLVPLVAPLGVAFNVNPVHLGVIFLANLELGFLC
                     PPMGLNLFLSATRFQKPLPHLYRQALPFLFIMTIGVLFITYVESASLGLLHLLGKG"
     gene            12196..13167
                     /locus_tag="PKL17_12335"
     CDS             12196..13167
                     /locus_tag="PKL17_12335"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HNK45568.1"
                     /translation="MHAGFRDGGGDVRRRQHWLILPTLLAVLSSGCSIKGYALKATAD
                     ALSSTGGGYGTEDDPDLVKQSAPFGLKTMETLAESLPQHRPIRLALASGFVQYAYAFV
                     NQDADRIADKSIAQAKKEWMRARRLYLRGRNYGLDALELAYPGFRKSLLSGNAGEAKA
                     ALQRVGKDDVPYLYWVGAGWALAISTGKDDPQLFGDFGLVGQLMERALELDESFDEGS
                     IHEFFVSYDSSRSEDQGGGPAQAKKHLDIAQKQGGGKKLGSLLSYAEGILLQQQKKAE
                     FVKLLEQIVAADVYSEDPTWKKNRLANIIAQERSRWLLGKLSDLFAD"
     gene            13282..14286
                     /gene="dctP"
                     /locus_tag="PKL17_12340"
     CDS             13282..14286
                     /gene="dctP"
                     /locus_tag="PKL17_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNK45569.1"
                     /translation="MKKQGICSLLTALLLGSAATASAQDKVVIKFGTRAPVGSTWHNS
                     LKEAAEKWREVSGGKVELKIFAGGTMGDEGDMIKKMKVGQLQAVGVSTVGLHVVAPDP
                     QAIDVPMMVSNRDEYRCALGKMAPKFEKALEAKGVVVLAWGEIGFTRFFSTESRPTLA
                     AMRTSKMFSWEGDPDSVTAWKNAGFKPVVLSSADLVPSLQVGKIDALCYPPVLVLAAG
                     MHNKAKFMLDFPYSSLTGVTVVDKNTWEKVPADLRPKLLEIFRSASVKIDQEVVKMET
                     DSLAKLVQQGTQKVPVQDLGEWQKAMETVYTDIRGKVVPAQAFDDVRQAIKECRAGKK
                     "
     gene            14312..14494
                     /locus_tag="PKL17_12345"
     CDS             14312..14494
                     /locus_tag="PKL17_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45570.1"
                     /translation="MRSLLAPPRNGRILAVRWGLNPNSSSLGVDITFLLFGLTIITIL
                     TPVVGFFLRYYRQRSP"
     gene            14609..15802
                     /locus_tag="PKL17_12350"
     CDS             14609..15802
                     /locus_tag="PKL17_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNK45571.1"
                     /translation="MERIRPAVSYVLVTAASALGLWAAFSLPHRAPAPPPNVVIAKPQ
                     PVVELQKPQQRPQVDVVFVLDTTGSMSSLLSGAKKKIWELARFIAQGQPAPDLRVGLV
                     AYRDVGDEYVTKFFDLTDDIDGVYQNLTSFVAGGGGDTPEHVAKALHEAVYRSNWSNG
                     KNALKLVYLVGDAPPHTDYQDGFDYRAIAKEAQRRGIRINTVRCGSDEDTRTNFTEIA
                     SLGGGDFSTIDQSGGMNDVRTPYDEELAKLNRALTDTAIPWGDADRRSSIRKKAETNL
                     AAPAETQAARAGFFGLAATRGRAAAVSEGDLLDDVANNKVQLERVAPSALPEPMQAMN
                     AEEKSRYIEGKRKAREEVLNQINSVAAKRDAYIKSSAAPAKSGFDGRVRETMKKQAAD
                     VGLMY"
     gene            complement(15799..16746)
                     /locus_tag="PKL17_12355"
     CDS             complement(15799..16746)
                     /locus_tag="PKL17_12355"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNK45572.1"
                     /translation="MIKALSIFSGNANRELFESICKVMEVSPGASLVSTFSDGETFVE
                     IRENVRNVNCFVVQPTCSPVNQNLMELLIMIDALKRASAGSIVAIVPYYGYGRQDRKV
                     KPRTPISSKLVADLITAAGADRLVSIDLHAGQIQGFFNIPVDHLYAMPVFIDHLRSRG
                     FVKEDAVVISPDAGGVERARAFAKRIESSMAIIDKRRPQPNVSEVMNIIGDVKGKHAV
                     IIDDMIDTAGTVTQAAKVILDRGAKSVMACVTHGVLSGPAIQRINDSVLSDVIITDTI
                     PLSAAARACPKIHVLSVAKLLAESIKRIHHGDSISSLFV"
BASE COUNT         3680 a         5315 c         4914 g         2963 t
ORIGIN      
        1 gttttcggtg tagaccttca tgcccggctt gcgaacttcg acaaggtgtc gccccgccgg
       61 cagatcgacg agctgcggca ccgtgccagc cggctgaccg tcgacgaaaa tctcagcgcc
      121 acgcgccgca tcgttcgtcg ccggatattt gatatcgagc cgagccggcg ctttgtcgag
      181 ctggaacgcg aacttcgtcg ccgcgccgat cgtcaccgtc tgctcaaacg gcctatatcc
      241 ttcaagctcc aggcgcagct tgtgctggcc tttgttgatt cgcaccttgg tcgcgggacc
      301 ggtgcgctcg gactgtagcg ccccatcgac gaagatcgtg gcccccgggg ggttcgagtc
      361 aatcgtgatg cggggcgcca cctgcgccga cgctgtttgg gggtggacga tggccaaaag
      421 ggccatcaac agagcacatg ccgtaatcct gatggggcta gcgagcatcg gtacaggttc
      481 tcctgctcca aagtcgagat tttgccgcgc catcgtaaga tcctcgtgcc atgggagtgt
      541 agttggtcct caaggtaaag ggcgcgcagc tattagcttg tcatttcacc aaatatttcc
      601 cgcaggttca acaggaacgg tttggtccgt tatctaagtt ggcttgtcat cttcgctaga
      661 tgaggccctt tccgcactgt tttgtgagag tgcccgaccc cccaacgtaa acagcaccaa
      721 aagacacgag cccatcaaga tcgccgtgca accactgttg acgatggctt tgacccggct
      781 cggatcgcgg ctgaagttgt tccagatcga ctgccagccc gcggtcagcg tcgttgcccc
      841 caccgcgagc atcggaatcg ccgtcaccaa agcgtagtgc cgccgcgtcg gatgcagccg
      901 caaaatgacc gccgtgccca ccgccagtgc cgtcgttgcc aagagctgat ttgaaatccc
      961 gagcagcggc cacagcgtcg aaatcgtccc ggtatacaga aaaccacccc agaacatcac
     1021 gaccaggatc gaggccagcc acacccccgg cacgaagtca tgtcgagaga agcgatcatc
     1081 gacacgcccc aagatgtcct gcatcagaaa gcgcgcgact cgcgttcccg cgtcgacggt
     1141 ggtcaaaata aacaccgcct cgaacatgac gatgaagtgg taaaagtagc ctagcagcgt
     1201 ccgcgctccc ggcagctgcg caaaaatctg ggcgatgccg gcggccaaac tgaccgaacc
     1261 gccggtgcga ccgcgcagtt tctcaccgac gagctgcgac atcagatcga tctcgaccgg
     1321 ggtcatcccc agcttttgaa acgccgcttc gggcacgttg atcgcaaagt aatccgccgg
     1381 atgcagacac gccgccgcga cgagcgacgt gatccccacc agcccttcga gcagcatcgc
     1441 gccgtagccg atcggtcgac agtcggtttc ctgatcgata agtttcggtg tcgtccccga
     1501 tgcgatcagc gagtgaaagc cacttatcgc gccgcacgcg acggtgataa acacaaacgg
     1561 gaagaccttg cctttgatga tcgggccgcc gccgtagaca aactgggtga acgcttccag
     1621 ctttagctcg gggtttacga ccatgatcgc cacgatcagc accgacagcg tccccagctt
     1681 catgtacgaa gacaggtaat cgcgtgggac aagcagcagc cagaccggca gcaccgacgc
     1741 aagcagtcca tacgccgcca cacacgcgac cagcgtgcca aacgacagcg acaagtacgg
     1801 tgccagccgc gagtgcgcaa tcgtgtcgcc ataaaccacc gcaaaaagca gcagcccgac
     1861 gccaagcagc gatgccgcgc gcagccgaag accgtggctt tcgccgcgca tgatcgaacc
     1921 catcaatagt gcgatcggga tcgtcatccc gatggtgaac gtcgcccacg ggctgccttc
     1981 gaggatcttc accaccgcaa agctcatcgc cgaaatcgcg actgtgacgg taaaaaagat
     2041 cgccagcgtt gccaccgatc cagcccgtga gcccagctct tgtcgcgcca tcgccgccag
     2101 cgacttgcca ccggaacgaa cgctcgctga cagcgtcaca aagtcatgca ccgccccagc
     2161 cagcgtcacg cccaccaaga tccacaaaag acccggcaaa aaaccaaact gcgcggcgag
     2221 caccggccca atcagcggcc ccgcccctgt gatcgcggcg aagtgatgac cgaacagcac
     2281 ccaacgactc gtcggaatgt agtctttccc atcccgcctg gcgtgcgctg gcgtctggtt
     2341 tttgccgtcg agcgcaaaga ccttggccgc aataaacgcc gagtagaagc gatacgcgat
     2401 cgccaagata caaattgccg cgatgaagat cggtaaggcg tgaatccgca tagccctcct
     2461 cggtgtttgt gctcttaaag acccgctagc agaaagcgga gtgccgcagg cagacgccgc
     2521 gcccaggcgt cttcattgtg ggtcgccatc ggttccaaga cgtattgcag attcgcgccg
     2581 tcgcgaaagc caatgtcacg ataggtcttg gccagttccg cggtgttcat ccagtcgtcg
     2641 ccgccttccc caccgccatc gccgctgtcc acgtagacgc ggcttggctt gccgacgctg
     2701 gctttgaccg ccgaaaggat gtagcgacca tcccaccacg tcgacggaga aaaaatccca
     2761 atccgaccga acacgtcggg ctttttgacg ccagcgtagg cagtgataag cccgccaagc
     2821 gacgatcccg cgagtgtcgt tttggccgga tcgctcacga gccgtccact tagcaccacc
     2881 ggggacgtca gtccattcat caagggaagc agctcttcga cgacaaactt gaggtattga
     2941 tcgccgccac cgctgtctcg ataggtcgga tcgctgcttt tggtcggggt gtattcgtac
     3001 agacggcgat cggtattttc cggtccaatc accaccactt ccggcaagct gtccgacgga
     3061 tcgagcgcat caatcccggc atccagcgtc tgcgacacct gccaggtccg tccgccaaac
     3121 gcatagcgcg gatcgaacag gttctgtccg tcatgcatgc acaagatcgg atagcgagcc
     3181 tcgggatttt catcaaagct cggcggcaga tacagccacg ccgcgcgggt attgcccagc
     3241 accgtcgatc gcacgccagt gacacgcaga taacgaccct tgctggtgac aaagtgcgga
     3301 tagatatcga ctgtctggcc cggctcgacg acaaaatttt tgccgcgtga ccacgtcgcg
     3361 tcgtcgagaa gcggcttcca ttccagcgtc gagctgaccc cttttaaccg cgcctgataa
     3421 agcccgtcgc ggactttggt catggctttg ccgctcgtcc aggaaagccc ggacccgctg
     3481 ccgcggatgc tcagcttgct gtagcgcgcg tcgttccggg gatagtggac gcgcagcacg
     3541 gtgtcaccgg gcacgacgtt gagatccgat ccatcgtccg caagatcccc tccactgccc
     3601 agatcgctac ccgcagcggt tctgtgatcc ccgccggttt tgtcatcgtc taacggaaca
     3661 tcgcttgacg cgcagcccca aagacaaagc gacaacccag cgatgcagcc gacagctccc
     3721 gtcgaaaaaa accgcggcac agcggcgcga acccccatcg agatgtcgtt ttcccaacca
     3781 gatcgcagtc ggctctccat cccggcacga tagccggttt tccgagcaag ccaaatcccc
     3841 acgcgcgact tgtcacaagc aaacaggacc gcgccacggt cgtcggagaa aaaaaccgac
     3901 atgtcctagg ttgtggcaaa agctgcgctt gtgttatgac caagcctgct atgtgcgcag
     3961 tcatgtcccc ctgctagtgc agggcttgct gccgcggctg gcaaggcaaa cgaatggaca
     4021 agatcgtcat caaaggggcc cgcgagcaca acctgaagaa catcgacgtc gaactaccac
     4081 gcgacaagct ggtggtgctc acggggctgt cgggctcggg taagtcgtcg ctggccttcg
     4141 ataccatcta cgcggaagga cagcgccgct atgtcgaatc gctctcgacg tatgcgcgcc
     4201 agttcctcga gcagatgggc aagccggatc tcgactcgat tgatggcctg tcaccagcga
     4261 tctcgatcga acagaaatcg accagcaaaa atccgcgctc caccgtcggt acgatcacgg
     4321 aaatttacga ctatctgcgg cttttgtacg cgcgaattgg caccgtgcac tgtcatagct
     4381 gcaacctgcc catcgcggcg cagacggtgc agcagatggt cgatcgggtg ctgcggctgc
     4441 cgcagggcac gcgcttttcg gttttggcgc cgattgtgcg cagccgcaaa ggtgagttca
     4501 aaaaagagct cgattcgctg cgacgccaag gcctgcggcg ggtcagcgtc gacggcgagc
     4561 tgttcgatct atcggaagac accatcaaga tcgacaaaaa caaggcgcac accatcgaag
     4621 tctacgtcga cagactggtg gtcaaaagcg acgtgcggca gcggctcacc gaagcggtgg
     4681 agctgtcgct tcagcttgcc gaaggcatcg tcaaggtatc gccagtcatg ctcggggacg
     4741 atgcgctgcc cgacggcgtg ggcatcaccg acgaaaacgg cgacatcatt ttttccgaac
     4801 gctttgcctg tatcaactgc ggcgtcagct accccgaaat cacgccgcgg ctgtttagct
     4861 tcaacaatcc atcgggcgct tgtccagcct gcgacggcat cggcgccaag atgtttttcg
     4921 accccgagct tatcgtcccc aacacggatc tggcgctgcg tgaaggcgcg atcgagccgt
     4981 gggaaaagcg caacgggccg ttttttcagc agatcatcga agcgctcgcg cagcactacg
     5041 gttttgacat ggcggtgccg tggtccgatc tgccgcagaa cgtgcgcgac atcgtgcttt
     5101 acggcagcaa aggcgacgag gtcgagttct ggtttgaaaa aaatggccga cgccacacct
     5161 acaaaaaaga gttcgagggt gtgatcgcaa atcttgagcg gcggctttcg gaatacgaac
     5221 gtcgtcgtcg agaagaaggc accgctcccg ccggatcgga cagctttgaa gcggcgtatg
     5281 aagagttttc gccgtacatg aacaagtcgc cgtgcgagga gtgccaaggc acccggctgc
     5341 gcaaagaagc gcgctttgtc cgcgtcggca acctgcccat tcacgagctg accgccaagc
     5401 cgattcgcga cgcacaccgt tttttcaccg agctacggct cagccagcga caagccgaaa
     5461 tcgccgagcg aattctcaaa gaagtgcgcg atcggctcgg ttttttggtc aacgtcggtc
     5521 tcgactatct gacgctggat cgcacagcag cgacgctttc gggcggcgaa ggtcagcgga
     5581 ttcgtctcgc gagtcaaatt ggcagttctc tcgtcggagt tctctacgtt ctcgacgaac
     5641 cttcgattgg cctgcatcag cgtgacaacc agcggctgct ggaaacgctg ttccgtctgc
     5701 gcgacatggg aaataccgtc atcgtcgtcg agcatgacga agacaccatc cgtgccgccg
     5761 accacatcat cgacatgggc cctcgcgctg gcgtgctcgg cggtcgggtc gtcgccgcag
     5821 gtcgggtcga tgagatcatg tcgtcgacgg cttcactgac tggcgcctat ctatcgggcc
     5881 gcaaaaaaat cgaagtcccg cgcagccgtc gtcagtcgca gaaccgctac ctggtgctaa
     5941 aaaatggcaa agagcacaac ctgcgcgacg tcacggttcg ttttcccgtc ggtgttttca
     6001 cctgcgtgac cggcgtttct ggttccggca aatcgacgct ggtgatcgat accctcctcg
     6061 cggcactgaa gcaaaagctc aaccattcca agtgccaggt cggtcagcac gaaggcatcg
     6121 agggacttca ccacgtcgac cgcgtgatag acatcgatca gctgccgatc ggacgcacgc
     6181 cgcgatccaa tccagcgacg tacacgggcg tctttaccca cattcgcgat ctgttcgcgg
     6241 cgctgcccga cagcaaatcg cgcggctaca aagccggacg ctacagcttc aacgtcaaag
     6301 gcgggcgctg cgagtcgtgt cagggcgacg gcatcctgcg catcgagatg cactttttgc
     6361 ccgatgtcta cgtcgtctgc gaagagtgcg gcgggcggcg ctataaccgc gagaccctcg
     6421 aagtaaaata caaaggcgcc tcgattgccg acgtgctcga catgacggtg acgcaggcct
     6481 gcgagttttt gggccacatc ccgaaagtgc gccaaaagct ggaaaccctg cgcgacgttg
     6541 ggcttggtta tatcgcgctc gggcaggcag cgacgacgct gtcgggtggt gaagcgcagc
     6601 ggatcaagct cagcaaagag ctggcgaaaa agggatcggg ccgcacggtc tacatcctcg
     6661 atgaaccgac gacgggcctt cactttgaag acatcaaaca gctgctcgag gtgctaaacc
     6721 ggctcgtcga ttcgggcaac acggtcatcg tgatcgagca caacctcgat gtgatcaaaa
     6781 ccgccgatta tgtcgtcgac atgggcccag aaggcggcga aggcggtggc tccgtcgtcg
     6841 catgcggcac gcccgaagac attgcgcgcg tgccaagttc atacactggg cagtacctca
     6901 aaaaagtgct cgcgctgagc taggccgcca cgatgaagat tctgtcggtg acccagcttc
     6961 cgattccgga cgtcaaagtg gtgcgctttg cgcgcttttg cgacgagcgc ggctatttca
     7021 gcgaacactt tcggaccacc gacatcgccg cgcatccgga tcttgcgcac tttgcggccc
     7081 agccgattgt gcagtgcaac gaagccttct ctcgacgagg aacgctgcgt ggtctgcatt
     7141 ttcagtggca gccgaaaatg ggcaagctgg tgcggcctct ttgcggacgg ctgatcgatt
     7201 ttgcgctcga tctgcggctg tcgtcgccgt ggtttggaaa aatcattggc tacgacatgc
     7261 cagcagcgac ggaccgcgac ttcgccgagt ggatttgggt tccgcccggt tttgcccacg
     7321 gcacgctgct ccccgaggag acagtgatcg aatatttctg caccgcggcg tggaatccaa
     7381 acgccgaggc aggaatttcg ccgctcgccg acgatctcga ctggtccctg tgcgaccagc
     7441 aagtcgccaa gctgtatgcc gatgcggtga aaaacggccc acttctgagt gaaaaagatc
     7501 gcgtggctcc atcgctgact gcctggaaag acgatccgcg cgccgggctc atcaccgatt
     7561 aagcacagtc gggagaagat cactgtgcat cgacccctgc atcgacggca ggcggcagtg
     7621 aaaaatcagg cggcggtggc ggcgctcccg acggcagcac ttgcagcgcc gtggttgcga
     7681 cacccagcag ggaacgcagc gctgcgccgg tcacaaaccc ttcgaccaaa agctcgctgg
     7741 ctttgacttc gatctgcaaa tgatcgagca ccgtcgagat gcccatcagc ttgaagcgcc
     7801 aatcgctgcg cagcttgtcg cccacttgca aaatcgccgc gcgaagctgg gtcgcttctt
     7861 ccgctgatga aaccaccaat cgcccgtgga cctgcgggtc catgtccgaa gacagcgcca
     7921 gctgaatgtg ccgcggcgtc gggatgccgc cacgcagccg cagccggcct tgcacattga
     7981 atatctcgac gacaagcggt ggcccctgtt tcggcagtgc cctgcgcagc gccgggagct
     8041 gggcggtcaa gctgggcggt tccggcccga cagaagcgtc ggggttttgg ccatccgacg
     8101 gcgggcttgg caacggctcg ggacgcggca aaaacgccag caggtcgggc gcatcgaaca
     8161 aaagctcgcg atcgtcccag gtcggtaccc gtcgcgccgt cagcttgtca aacagcggac
     8221 gcagtttctt tcccatcgcc aaaagtgccg tcgcgctgcg ccggtatccc gtcggatcgg
     8281 tcgtcgcaat caccagcaca tccgacaaac tgaacagttc ctgcgccagc tcgtcgcctg
     8341 acgaaagcgc actgcccgcc accagcaacc gactatctgg aaacgccccg agcagaccgg
     8401 ccaccgactg acgatgcggc gagcgtgcaa cctctgccat gcgcagcacc agccgcagtc
     8461 ccacgtcctg ctcgatcgac gctgcgccta cgccatgcag actcgcgagc ggtggtgtct
     8521 tgggcggtgt tggcttgcct atcttgccgc ccggttcccc ccgtcgcgaa agctgctgtt
     8581 tttgcgcagg ctccggggga tctggcacaa gcggcttggc ggcgaccgct tcaggcgcaa
     8641 taagcggcgg gggcgtcttg cgcttggctg gcacaatgtc ccaggtaatc ggcggcggcg
     8701 gcagcagttt ttccaacagc gacagcgacg acaaaagaat cgccggtagc aaatgcgcga
     8761 gcagcgacag acaacccgcc aacacgcatc gccgcatccc acttgcgaag tccactgaca
     8821 ccacctgctc gacaccctag caaagccgcg gcgacgctcc tagcgccagc cggtcgtttg
     8881 cgccgttttg ccgtcgaatc gttcagtaaa ggcgcgcaaa aaacggctcg gcatgccggg
     8941 caaaggccgc atacgtgaag tggtcataca gtcgctgata cccggcgtcg cccaagcgct
     9001 gccgcagcga cggatcgcgc aagcgcaaaa tccccgcaca gaccgcctgc accgtcggtt
     9061 caacgacaac cccggtttcc ccatcgagca cgacttccgg cgcgccacca acgctggccg
     9121 caagacacgg cctgcgatgc gccatggctt cgagatagac gatgccaaag ccctcaccgc
     9181 tcgacggcaa caccaaggca tggctgcgcg caatcgccgc gtgcttttcg gtttccgaca
     9241 gccgaccgac aaaatggacc cggtctgcaa cgcccagcga ctgcgccaga tcgacaagct
     9301 gcggacgcgc cgcgccgtcc cccaccactt caaagtcgac atcgggcaac tgcggcaagc
     9361 tgcgaatcac cagatcgatg cctttgggtt cgccgatcca cagccgtgta atcgccacaa
     9421 ttcgcagccg cagcgacgat tcacaggcgg tctttggggt cgctgccgcc agtgccaggc
     9481 tctgttcatc gagcgcattg acgagccgct gacagcgatc tggccttaca ccttgcaccg
     9541 cgcagagctt gcgcagcgta tcttcactga cgcccgccgc caccgtcgat cgctgaaggg
     9601 accagcgacg caaaatgggc aacggcgacc agacatcggt gccgtgcgca atcaccccaa
     9661 agcgcgaaaa gccaagcccg agcggcgaca ggttgacatg cccgagcacg acggcggatt
     9721 cccgaccgac aaagcccagc acttccgcag caaacagccg ccggttgccg cgacacgcca
     9781 cataagtcgg tgagaccgca cgaggcagcc cagccaaaaa gtccggatcg gcgcctggct
     9841 catcggtcag cgcgacgatg tgaagcggca ggcggcggac ttgggcaaac tcgctcgcgg
     9901 cgcgaatgag caagcgatta aacgacggaa tgccgcccgg agcgccgagc aggctgctca
     9961 aggccagcag catggcttag cccttgccga gcaggtgcag aagcccgagc gacgcgctct
    10021 cgacgtaggt aatgaacagc acgccgatcg tcatgatgaa caaaaacggc agcgcttggc
    10081 gatacagatg cggtagcggc ttttggaagc gcgtcgccga caagaacagg tttagaccca
    10141 tcggcggaca caagaaaccg agttcgaggt tggccaagaa gatcacgccc aggtggaccg
    10201 ggttcacgtt gaacgcgaca ccgagcggcg cgacgagcgg caccagcacg acaatcgccg
    10261 aataaatctc cagcacactg ccgagcacga gcaaaccgac gttgagtatc agcaagaaca
    10321 cccagcgcga atgaatgtgc gcctggaccc actcgacgag cttggttgga acctcggctt
    10381 cgaccagata gttggtaagc cccagcgcca ccccgagcag cacgaccacc gcaccgacga
    10441 gcgacgcagc atgcaaaaac gccgacggca ggtcttttat cggatggaca ttgcggaaca
    10501 ccaaaagctc gatgatgagc gcgtaggctg ccccaatcgc cgcggactcg acgatcgtcg
    10561 cgatgcccga cagaaacgaa cccatcacca ccaccggcac tacgatgtcc cacttggccg
    10621 cccacactgc ctcgcacgct tctttccaga cgagcggctg ccgcggcaca ttggctttgt
    10681 agccttgctg catgccatac aaagacacca gcacgaccaa acacagaccg ggtaggaagc
    10741 cggcgataaa caactggtca acggaaacag gagcgactga tcccggtgca ctcgccgcca
    10801 ccgaatacaa aatcactggc aggctcggtg ggaacaacag ccccaaactg cccgccgccg
    10861 tgaccatgcc gagcgagaac cccttgggat agccgtcgcg caccagcatc ggatagacca
    10921 aaccgcccag cgccaagatc gtcacgcccg acgcgcccgt gaacgtcgta aacaccgcgc
    10981 agacgatgac gaccatgatg gccatgccgc cgggaatcca gcccagcacg ctcttgtacg
    11041 cgcgcaccag ccgatgcgag gagcgtcccg acgccagcac atagcccgca atcgtcagca
    11101 gcggaatggc cggtaactgc ggattggcga cgagccgcag cgtttcattg ggcagcgatg
    11161 caatcggcgt cccggccaaa aaaaacaacg tcatcgccag ccccgccatc accacgaaca
    11221 ccggcagccc caaaagaaag ccggtcccca tcgaaaacag cccgagccac ttcaccagtc
    11281 cgacgagttt ttcggtcttc gcaaccagcg cttgcagctt cggcgagcca tcgtgccaaa
    11341 agccgatcac aaacatcaca atcgccaaga tcagcgtcac gccgcagacc agccagcgaa
    11401 gctttcgcct tcgcggcgac atgtcgtggt gcggttcgtt tggacttggc gccccgtgac
    11461 caccgccggc attcggatcg agcgctcgcc acgccgcccg cggccccatg agcgcaaaca
    11521 ccaccgggat gatgatctgc acgtaccact tgtgcagtcc gccggggagc aggtcgtttt
    11581 ctcccgtcac acgctcgacg gccaccatgc gagcactggc atacgtcaac accagcgcga
    11641 ccagcacaaa taccgtcgac gtaaagcctt cggcggcact gcgcatccgt cctttggaca
    11701 gaaaacccgt cgtcgacaag cccaagtgct tgccgcccgc caccgcgagc agcgccccca
    11761 aaaagccgat ccagaccgtt ccgttctgta cgtacagcga gacccacgac agatccgacg
    11821 gaaccagctt gtgcaggaca aacccgagcg agtgagtaag ccacttcagc cgatggcttt
    11881 cgcgcagcat gtcagcgagg ctgcgcaggc tcgacagcac tggtaaaaat acgatcgcca
    11941 acgacaacag gcagatcagg attttttccg gtaacgtacc agccggcggt ccttcgccgg
    12001 tgccctcgac cgcggccatt tccagtggcg gttctggtgg tgcggcggtt tctctggcat
    12061 cgccgggcgg aggggaagtg ggcgacgcgg ctgggctttc aggcgaggat tcgaccatat
    12121 gaagtgaaag cagtatacca ataggacacc tgtggtacag tgccgtgacg ttttgggaac
    12181 caaaggcaac aatcgatgca tgctggcttt agagacggtg ggggcgacgt tcggcgcagg
    12241 cagcactggc tgattctgcc aactctgctt gcggttctgt cgagtggttg ttcgatcaaa
    12301 ggctacgcgc tcaaggcgac ggcagatgcg ctgtcgtcga cgggcggtgg ctacgggacc
    12361 gaagacgacc cggatctggt aaagcagtcg gcgccgttcg gtctaaagac gatggaaacg
    12421 ctggcagaaa gcctgccgca gcatcgtccg attcgcttgg cgctcgcgag cggctttgtc
    12481 cagtacgcct acgcatttgt gaaccaagac gcggatcgaa tcgcggacaa aagcatcgcc
    12541 caagccaaaa aagaatggat gcgcgcgcgg cggctgtacc tgcgcggtcg caactatggg
    12601 ctcgacgcgc ttgagctggc gtatcctggt ttccgcaagt cgcttttgtc cggcaacgcg
    12661 ggcgaagcca aggccgctct gcagcgggtc ggcaaagacg acgtgccata tctgtactgg
    12721 gtcggggcag gctgggcgct cgcgatcagc accggcaaag acgatccgca gctttttggt
    12781 gactttggac tcgtcggcca gctgatggaa cgagcgctcg aactggatga gtcgttcgat
    12841 gagggctcga tccacgagtt ctttgtgtct tacgactcct cgcgatcgga agatcagggt
    12901 ggcgggccgg cacaggccaa aaaacacctc gatatcgcgc agaagcaggg cggcggaaaa
    12961 aaactcggct cccttttgtc ctacgcggag ggaatcctgc ttcagcagca aaagaaggcc
    13021 gagttcgtga agctgctcga gcagatcgtc gccgccgatg tgtattcgga agatccgacc
    13081 tggaagaaaa accgcctggc caacatcatc gcgcaggaac gctcgcgctg gctgcttggc
    13141 aaactatccg acctgtttgc ggactaactg cggcatcgaa atgtttttcg atagccttcg
    13201 tcttatccga tacaagcgaa acatctgatg taatgaagcg aggacttttt ttacgaagag
    13261 tcagcttctc aaggaacacc catgaagaaa caaggcatct gctcactgct cactgccctt
    13321 ctgcttggta gcgcggccac tgcgtcggcc caagacaagg tcgtcatcaa gttcggaacg
    13381 cgcgctccgg tcggctcgac gtggcacaac agcctcaaag aagcggcgga aaagtggcgc
    13441 gaagtcagcg gtggcaaagt cgagctcaag atattcgccg gcggcacgat gggtgacgaa
    13501 ggcgacatga tcaagaagat gaaagtcggc cagcttcagg cggtcggcgt cagcaccgtc
    13561 ggactccacg tcgtcgctcc cgatccgcaa gcgatcgacg tgccgatgat ggtcagcaac
    13621 cgcgacgagt atcgctgcgc gctcggcaaa atggcgccga agttcgaaaa ggcgctcgaa
    13681 gccaaaggcg tcgtcgtgct ggcgtggggc gaaatcggct ttacccgctt cttttcgacc
    13741 gaatcgcgcc cgaccctggc ggcgatgcgg acctcgaaaa tgttttcatg ggaaggcgat
    13801 ccggacagcg tcaccgcgtg gaaaaacgcc ggttttaaac cagtggtgct aagcagcgct
    13861 gatttggtcc cgtcgctgca agtcggcaaa atcgatgcgc tgtgctatcc gccggtcctg
    13921 gtgctcgccg ccgggatgca caacaaggca aagttcatgc tcgacttccc gtattcgtca
    13981 ctgaccggcg tgacggtggt ggacaaaaac acctgggaaa aagtgccagc cgatctgcgc
    14041 ccgaagctgc tcgaaatctt ccgctctgcc agcgtcaaga tcgatcagga agtcgtgaag
    14101 atggaaaccg attcgctcgc caagctggtg cagcaaggca cccaaaaggt gccggtgcag
    14161 gatcttggcg agtggcaaaa agcgatggag accgtctata ccgacatccg cggcaaggtg
    14221 gttccggcac aagcgtttga cgacgtgcgg caagcgatca aagaatgccg cgccggcaaa
    14281 aagtaagcgc gcttcgttgg gagtgaggcc tttgcgcagc ctgcttgctc caccgcgaaa
    14341 cggacgaatc ttggcggtgc gctgggggct caacccgaac tccagctcgc tcggcgtcga
    14401 cattaccttc ctgttgtttg gtctcacgat catcaccatc ttgaccccgg tcgtaggttt
    14461 ctttctgcgc tactaccgtc agcgttcccc gtaagcacag ctcctgagcg acggtggctc
    14521 ggtcttttgg accgcctcgt gccaagctag atcgactcgc cttgtgccgc gttgttgccg
    14581 ataagcaagc acgcaggaga agctgtcgat ggaacgcatc cgacccgctg taagttacgt
    14641 ccttgtcacc gctgcctcgg ccctgggcct gtgggcggcg ttttcgctac cccaccgcgc
    14701 cccagcgcca ccgcccaacg tggtcatcgc caagccgcag ccggtggtcg aactccaaaa
    14761 gccgcagcag cgtccacaag tcgacgtggt gttcgttctc gataccaccg gatcgatgag
    14821 ctctctcctg agcggcgcca aaaagaaaat ctgggagctt gcccgcttca tcgcccaagg
    14881 ccagcctgcc cccgatctgc gggtcggtct ggtcgcttac cgcgacgtcg gcgatgaata
    14941 cgtgaccaaa tttttcgacc tgaccgacga cattgatggc gtgtaccaaa acctcacgtc
    15001 atttgtcgcc ggcggtgggg gcgatacccc tgaacatgtc gccaaagcgc tgcatgaggc
    15061 ggtctatcgc agcaactggt ccaacggcaa aaatgcgctc aagctggtgt atctcgtcgg
    15121 agacgcgccg ccgcacaccg attatcaaga tggcttcgac taccgtgcca tcgccaaaga
    15181 agcccagcgc cgtggcattc gcatcaacac agtgcgctgc ggcagcgatg aagacacccg
    15241 gacaaacttc accgagatcg cctcgctagg tggtggtgac ttttcgacga tagatcagtc
    15301 gggtggaatg aacgacgttc gcacacccta cgatgaagaa cttgccaagc tcaaccgtgc
    15361 gctcaccgac acggccattc cgtggggtga cgccgatcgc cgcagcagca tccggaaaaa
    15421 ggcggaaacc aacctcgccg ccccagccga aacccaggct gcccgtgccg gcttttttgg
    15481 gctggccgcc acacgcggac gcgccgcagc cgtcagcgaa ggcgatctac tcgacgatgt
    15541 ggccaacaac aaagtccagc ttgagcgcgt cgccccaagt gcgctgcccg aaccgatgca
    15601 agccatgaac gccgaggaaa agtctcgcta catcgagggc aagcgcaaag cccgcgaaga
    15661 agtgctaaac caaatcaact ccgtcgctgc caagcgtgac gcctacatca agagcagcgc
    15721 cgcccccgcc aagagcggtt ttgacggcag agtccgcgag acgatgaaga agcaagccgc
    15781 ggacgtgggc cttatgtact aaacaaacag gctcgagatc gaatcgccgt ggtggatgcg
    15841 cttgatcgac tcggcgagca gcttggccac cgacagcaca tggatcttgg gacaggcccg
    15901 cgccgccgct gacagcggga tcgtgtcggt gatgatcacg tcggacagca ccgagtcatt
    15961 gatgcgctga atcgccgggc ccgacagcac gccgtgcgtc acacacgcca tgacactttt
    16021 ggctccgcga tcgaggatca ctttcgcggc ctgcgtcacc gttcccgcgg tatcgatcat
    16081 gtcgtcgatg atcaccgcat gtttgccttt gacgtcgccg atgatgttca tgacttcgga
    16141 tacgttgggc tgcggacggc gcttgtcgat gatcgccatc gacgactcga tccgcttggc
    16201 aaaggcgcgt gcgcgctcga cgccgccggc atccggcgaa atcaccaccg cgtcctcttt
    16261 gacgaagccg cggctgcgta ggtggtcgat gaacaccggc atcgcataca ggtggtcgac
    16321 gggaatgttg aagaagccct ggatctgacc tgcgtgcaga tcgatcgaca ccagacgatc
    16381 agcaccggct gcagtaataa ggtccgcgac cagcttgctc gatatcggcg tccgtggctt
    16441 gaccttgcgg tcctgacgac catagccgta gtacggcacg atcgcgacga tggagcctgc
    16501 cgacgcacgc ttgagcgcgt cgatcatgat gagcagctcc atcaagttct gattcaccgg
    16561 cgagcaggtc ggctgcacga cgaagcagtt gacgttgcgg acgttttcgc gaatctcgac
    16621 gaaggtttcg ccgtcggaaa aggtgctgac gagggacgct ccgggcgaaa cctccatcac
    16681 cttgcagatc gactcgaaca gctcgcggtt cgcgttacca gagaagatgc tgagggcctt
    16741 gatcatgaga agtggttctc gaccggggcg agccctccag atgcggatga acaagcacca
    16801 tgcccgcaga tcagaacagt tggggcggta ggattcgaac ctacgaatgc gggttccaaa
    16861 gacccgtgac tt
//
LOCUS       DAORGN010000103        16831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1385_length_16831_cov_6.398426, whole genome shotgun sequence.
ACCESSION   DAORGN010000103 DAORGN010000000
VERSION     DAORGN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16831
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1385_length_16831_cov_6.398426"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKL17_12360"
     CDS             <1..406
                     /locus_tag="PKL17_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45573.1"
                     /translation="RRPWEVLMHRRFSPRTLAAVVIAVSSLIATSCYAPDISRLLYRC
                     QGSDPCPSGMVCNFGLCVASDIAACGTGIGVWLTTDDYKALCPAIGPATFNSCSDNYE
                     IDKNCPEEASTACLDPITMKVNPDCFRCCRKK"
     gene            447..1463
                     /gene="holA"
                     /locus_tag="PKL17_12365"
     CDS             447..1463
                     /gene="holA"
                     /locus_tag="PKL17_12365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNK45574.1"
                     /translation="MAPSTASLDPLAPLLSALASAPPPLVFLTGKERFLIDRAVEAIK
                     AATLSPATKDFNYDQLFAKEAGAAKILACAKTLPMMAKKRLVLVRDADELSADEMTLL
                     GKYVESPAPETCLTFLAEKVDLRLRFFNLFKKHGLLLKLDPLADKQLPGFVEAEARRL
                     KVDLQPGTAARLAEEIGADLGQLADALERLCCYVPVGQPIRIADVEEVVVTTRQHSVF
                     ELIDAVGGGDRSQALTLLSDMLMQREPALKLLALISRHVMKLWQTADLLSQGRPSASE
                     IAGALGVMPFVAGKLLDQARRLKLSRVQAMHEAIFQTDRQLKQSKLDDERIMEQLILG
                     LCAT"
     gene            complement(1473..2420)
                     /locus_tag="PKL17_12370"
     CDS             complement(1473..2420)
                     /locus_tag="PKL17_12370"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNK45575.1"
                     /translation="MSLQAGEVAVRIYGSALGAFPGAESVGVVEEAGGAAQHLVGQAV
                     LVPRLLPCGECPACRRGAVRACPQRRNRPVRPCLREVVPARALLPLVPPFVDASPATE
                     NLWPYAVLSDALLTPYSGFVRAGVCAGTVCVVLGQGLRADLTALVADSLGLVVERLSP
                     SDEERLDPAAARREISVRAAAQGLALAGAVLVETVGSLAARARAVQMAEPAGTVLLYE
                     PSQPLDGSAGQLVAEPQAGAALSSLAALAHIVDMQAQLIAVAPHPDLLPELLSLCART
                     QIDFTKLTREVSPSDMDSVMAARRCGQDGDSRLPVVRFP"
     gene            2524..3507
                     /locus_tag="PKL17_12375"
     CDS             2524..3507
                     /locus_tag="PKL17_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNK45576.1"
                     /translation="MYEACSTCGERFIPQYAYQVATLPAAPGEAPRRQYFCQLTCRRT
                     ALGEVTHAVRRARRMAILNQKGGTGKTTTAINLAAGLAERGFETLLIDTDAQGNVGAS
                     LGVKGDRSLYHVIVDGADPAEVAVPVRGRLDVITADATLAAAEVWLARRDQDRDRVLS
                     NRMGQAHRRYQYVILDCAPSLSLLNQNALTYADEVLIPVSCDYLSLVGVKQVLKTLKD
                     IERHLGHSVRIAGVLPTFYDSRIRLAREALETLRGHFREKLMEPVRRSTRLAEAPSHR
                     QSIFEYDGESPGAEDYRRLVERVIGKEQATAPLGTRPREIVQPSMEPATLE"
     gene            3521..3859
                     /locus_tag="PKL17_12380"
     CDS             3521..3859
                     /locus_tag="PKL17_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45577.1"
                     /translation="MSLKNSAPRALQTDPLSVDDILATGFYRPEREADRADPREAVHE
                     LPAGQVPEKKPRPTHYKVVCISLYNEDIERLEMLVRELKRRGYSKANKSLVIRAALEQ
                     LDVEKVPRFH"
     gene            3876..5030
                     /locus_tag="PKL17_12385"
     CDS             3876..5030
                     /locus_tag="PKL17_12385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNK45578.1"
                     /translation="MSTQRKLHVAVLYGGRSGEHEVSLQSAASVLRNLDPARFVAVPI
                     AIDKDGRWHPGDVSRIEKGARTLPVFREQPQVVLPPNPPQSAGAQLVPLETTTLVGLG
                     LPLPPIDVVFPVLHGTYCEDGTLQGLLELADVPYVGCGVLASAIGMDKEVAKRLAGLA
                     GIPVVPYLTVHSRTYAAQASTLPAEVGHALGYPCFVKPSGSGSSVGVHKVKKPEDLDA
                     AVRDALRFDRKVLIEKAIDAREIEVAVLENSDPSGEPLCSIAGEINPRHEFYSYQAKY
                     LDENGAALLVPAPISPAQLAEVKKWAALAFTTLECEGLARVDFFLDRKTGQFYFNEVN
                     TLPGFTSISMYPKLWEASGISYAELLSRLVDLALRRGQEKKALIHDFAAI"
     gene            5131..5697
                     /locus_tag="PKL17_12390"
     CDS             5131..5697
                     /locus_tag="PKL17_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNK45579.1"
                     /translation="MAQAEGLAKMDAQGSDKDGGCGQDASAGTPKRRRTAKSEHLITL
                     KAMVDGAKTTPRAVRLYEAEKLISAAQRSSGGHRLFDEGELDKLRLIIDLRACGFSIE
                     EIRELLAARQTSPSKESAMALQALLSRHIEGLKRKLALITELGREFQCAVETLDRCAT
                     CDNPAGKSACLSCEVLTSRAAPRCMRFI"
     gene            complement(5737..6582)
                     /locus_tag="PKL17_12395"
     CDS             complement(5737..6582)
                     /locus_tag="PKL17_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45580.1"
                     /translation="MLLGPALLLGCALAASSTVVGSDRACEVVQSTQSALLEAGLDGE
                     IRQNYARGMAELRRYNLRDGLSLIRRARALAYRELLHGPLRGPLPRRHFVRLAYVEGQ
                     VQQLSLIDEQLKLRADRGGEQAAAIQLRALLLHNVFLAVRGFTGQIDVAFADRVLAAY
                     REALGLRSRFNREIQIGNAAMLAERGELAEARQEFAKIPDSELTGDGTDLSVIYYRLA
                     VGETGLAVQRLLEAARRDTWHHGAREGSSLRASMYRMNDFDRLRAHPRFVDLVTVPEE
                     ENPLQ"
     gene            complement(6586..7446)
                     /locus_tag="PKL17_12400"
     CDS             complement(6586..7446)
                     /locus_tag="PKL17_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNK45581.1"
                     /translation="MSVLKAAVVQLCSTADVERNLAQVEALCQKAQRRGAELVCLPEN
                     FALLSSDEKDKFSHAVTIAAKSDGTLHGHGQILGRMQALARSLSVHLVLGGLPEVGQT
                     PSRVFNSSVHLLPDGNIAAVYRKIHLFDVHFFHAATSLCESATVEYGAVDQVIVSETP
                     WGGLGLSVCYDVRFPELYRRLSAAAAKMLSIPAAFTLHTGKDHWHVLLRARAIENQCF
                     VLAAAQQGRHSPSRFTYGHSLIVDPWGTVLCECPDGEGVAVAELDFESLQKIRTDLPA
                     LRHRRIGVKD"
     gene            7490..8533
                     /gene="hrcA"
                     /locus_tag="PKL17_12405"
     CDS             7490..8533
                     /gene="hrcA"
                     /locus_tag="PKL17_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNK45582.1"
                     /translation="MLELNNRARKILSAITQEYLSTGEAVGSRTVTRRYGVDLSPASV
                     RNVMADLEELGLIRQPHASAGRVPTDLGLRFFVDSLLKIRQLSSRDREEMAGHYNFST
                     GEVDAVLREAGQVLSDLSTHTVVVMTPRVDLDVLKHIEFVRVKDNAVVAVLVNTSGRV
                     LNKLVTLDEPIAPDLLDRITNYLNSFLDGKTLSEARSLIEKELAKERTRYDAQAKLAL
                     RLSAQALPAEPPSVIVSGQANLLSHVLSEEAAPTPERLKALFRTLEDHRQLLRLLEQT
                     EAAHGIQVFIGAETSVPEFSDKTVVAASYGPNEKPVGALGVIGPTRMNYSRVIALVDF
                     TAQLLSGVIAQNR"
     gene            8614..9216
                     /gene="grpE"
                     /locus_tag="PKL17_12410"
     CDS             8614..9216
                     /gene="grpE"
                     /locus_tag="PKL17_12410"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNK45583.1"
                     /translation="MATEEAQSSNAPESSGQAQSGQPPSAASPEADLKAQVDKLSEEK
                     RDLNDRLLRLAAEFENYKRRARKEQEDAAARGLESLVRELLPAMDNFDRALSASKNPS
                     TTVTAIVEGVQMVQRSFFAALEKSQIRPFEAEGKPFDPNFHEAIGQVESATLPPGHVG
                     NVFQRGYMIGSRLLRPALVQVTRKSEQAPETPPSTPGQAN"
     gene            9225..10412
                     /gene="dnaJ"
                     /locus_tag="PKL17_12415"
     CDS             9225..10412
                     /gene="dnaJ"
                     /locus_tag="PKL17_12415"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNK45584.1"
                     /translation="MADAKRDYYEVLGLPRDAEPTEIKRAYRALALRYHPDQNQNDKH
                     AEDKFKEVSEAYTVLSDPEKRARYDRLGHLGLSRDVSGIDPLSVDLESMKSLFSNLFG
                     DLIGGKKGKGRDLRYNLDISLSEAAHGVLKTIRFPVRADCPSCHGSGARGGDAGLRTC
                     TTCAGKGEVKPGKGLFSLSRPCTVCQGVGKVVVDPCSECRGAGLTEQQREYTVSIPPG
                     TEDGSARRISGQGEPGRRGGGGAGDLNVTVKVEPHPILRREGALLLCDLPITLTQAAI
                     GCALDVPTLEGRVEMKIPAGTQSGTVFRLRSRGFPTSVGATSRGDLHVKVFIETPGNL
                     SEAQRALLRHIEDKLGGADYPRIRKYQDDLKHLYGPADTTTTPITKSDGKPATKPNNK
                     PVS"
     gene            10684..11805
                     /gene="recA"
                     /locus_tag="PKL17_12420"
     CDS             10684..11805
                     /gene="recA"
                     /locus_tag="PKL17_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNK45585.1"
                     /translation="MAIEKKDDAKTPSRSERLAALDAVTSNIEKQFGKGSIMRLGEGM
                     ALPEVVVMPTGSLALDIALGCGGLPRGRIVEIFGPESSGKTTMALSVLAECQKRGGMA
                     AFVDAEHALDIVYAKKLGVRTDDLLVSQPDNGEQALEIADMLVRSSAVDVVVIDSVAA
                     LVPKAEIEGEMGDQHVGLQARLMSQALRKLTGSINKSNTLVIFINQIRMKIGVMFGSP
                     ETTTGGNALKFYASQRLDIRRVGALKQGEKVVGNRTRVKVVKNKLAPPFREVEFDLMY
                     GEGISHEGDLVDLGSEAGIIEKSGAWFAYGGERIGQGRENAKEYLKTHPEMADAIEAA
                     ILEKHGIKRQGIDPSSTAAAASTAEHVAPPTGRKAKAAS"
     gene            complement(11812..13599)
                     /locus_tag="PKL17_12425"
     CDS             complement(11812..13599)
                     /locus_tag="PKL17_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45586.1"
                     /translation="MSTGLPRDKIGPYRLVRLIGKGGMGAVYEAIQEPIERRVAIKVL
                     HGRYAQEPEIAQRFFNEARAVNIVDHPGIVQISDYGQLPSGVAYLVMEFLKGDTLGQR
                     LKANGGKLAVPEVVRISRQIAAALAAAHEKGVIHRDLKPDNIMLIADPDPEAPGRIRV
                     KLLDFGIAKVAAELNQGAAQTAADVVMGTPKYMAPEQCRGAGNVDDKSDVYSLGVIMY
                     EMLVGKPPFTGAQGEILAKQIYDEPPPIREAAPHVPVNLGQVVHRLLIKNKADRPTMR
                     QVAGELEGLAVAYPTTAHAIVRMPPGFQLSSANIPIPGFVQPGQASPSPQAASGAMVP
                     AAQSSPGDDDTPTGNRLKVAKEKADKDSNSGLRQSTLAGATGQSSASMTPARRRRWIA
                     VVAAATIVVSSLVVAITVRFLRDEREGNQPAVVQTKIERRNVMLSVESTPAGADVLLA
                     SSGRSIGLTPMKKDWPRGKEPLKLIIRRPNYKDQTVDANVGSDVSLNVPLEPVDPPKP
                     ATGSKPAPTPPAGKNGGATKSTGPAAGKTGPSGKVPAGTSPAGKSVAVKNPIPPTGTT
                     KAGPGKTTPVAGGKPTGKTKPSGRPEIVD"
     gene            complement(13685..14431)
                     /locus_tag="PKL17_12430"
     CDS             complement(13685..14431)
                     /locus_tag="PKL17_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45587.1"
                     /translation="MKKSSARGEKTMRKRLGIQGLCVGLLLLLQGCASMRVAKVKTWE
                     AEGNDSALKAALADDSDAVQEAAIAAWVHYGEYDTGRPSVLAALQSSSAPAARQAEKR
                     LFGQPPIAVPDPHPFAQASGSTIIYFYRDADDAGDGMWMVVDDSEKVRLLRGRYFRYA
                     AGTGSHRACLQLPAVAAPVTDDPSDDGGQLRKVAPQCSTLSAHTAGVYFVRYRGDAGR
                     GRPDLKIMPIPVGLSAVKSLSAVEPENRKK"
     gene            complement(14428..15048)
                     /locus_tag="PKL17_12435"
     CDS             complement(14428..15048)
                     /locus_tag="PKL17_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45588.1"
                     /translation="MTACASWLLRHGAGFWGRLWIGLLLLLVGCGQRRTAESACLALR
                     DAIWEADGIAVYDVLLQNTQWSVSTVNKLHRQMATQIRASYPAAQREAALGRLFASSA
                     DSGRELFLSLYGERYAADFRARVAEDMRTVPVVGDVVHCGCAAGKPFLLARGLDGKWG
                     LSELDREWEDAKLRAFHDLETVEHNVALFQKVGSQTATFDREAGSR"
     gene            complement(15045..15473)
                     /locus_tag="PKL17_12440"
     CDS             complement(15045..15473)
                     /locus_tag="PKL17_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45589.1"
                     /translation="MRSQTSYLVVMMCMMGVFAIVRVQPFGLMLAPGNLVVGMALLLG
                     GLQLGFQRVFSLAIPTGAALIAMVGGLLTYGKVRGFELPGFPLIWLIVGLYIEMRLVL
                     HMQNLRRQELAQKRSVSEANKEELVEKAAESTKDGDKPSA"
     gene            15757..>16831
                     /gene="dnaA"
                     /locus_tag="PKL17_12445"
     CDS             15757..>16831
                     /gene="dnaA"
                     /locus_tag="PKL17_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNK45590.1"
                     /translation="MLTDLPLDSSASGNPPSPAGTTMLWDAAMDILSRKLSAQNFDLW
                     FRPIRCQRIDGAHIHLIAPNQFIKEWFETHYQKVVLDVIGESSDRRFTIHWGIDDGAH
                     ESERGRGDETAAQPTVPEKPAAKKPSPSVDTARSAAARRSGTNGKAASQSDGAEPGPE
                     LLARYVFDSFVIGPSNQLAAAASRAVADHPGSKYNPLFIYGGVGLGKTHLLHAIGHKI
                     QADHPGWRILYLKTETFMNEYINQVRSGRIEEFRAKYRDTTDVLLMDDIQYLGGKERT
                     QDEFFHTFNALFESHRQIVVTADKYPHEIPDLEERIRSRFQWGLIADIQAPELETRVA
                     ILQKKAELDQIALSDEVALFLATH"
BASE COUNT         3474 a         5371 c         4948 g         3038 t
ORIGIN      
        1 ccgtcgtcct tgggaggttc tgatgcatcg acggttttcc ccccgaacac ttgccgcagt
       61 ggttatcgcg gtgtcatcgc tcatcgcgac aagctgctat gcaccggaca tttcgcgact
      121 tctgtatcgc tgtcagggga gtgacccgtg cccaagcggt atggtctgca acttcggtct
      181 ctgcgtcgcc agcgacatcg cggcttgtgg caccggcatc ggcgtgtggc tgaccaccga
      241 tgactacaaa gcgctgtgcc cggcgatcgg acccgcaacc ttcaattctt gcagcgacaa
      301 ctacgagatc gacaaaaact gccccgaaga agccagcacc gcctgcctgg atccgatcac
      361 gatgaaagta aaccccgact gcttccgctg ctgccgcaag aagtagccgc ttgaaaaccc
      421 agcgcgctca cctatagtcg ccggccatgg cgccgtcgac cgcatcgcta gacccgctcg
      481 ctccacttct ttcggcactg gccagcgcac caccgccgct ggtgtttttg accggcaaag
      541 agcgctttct gattgatcga gcggtcgaag cgatcaaagc ggcgacactc tcgccagcga
      601 cgaaagactt caactacgac cagctttttg ccaaagaagc cggggctgcg aaaatcctcg
      661 cgtgcgccaa aacgctgccg atgatggcca aaaaacggct ggtgctggtg cgcgatgctg
      721 acgagctgtc tgccgacgaa atgacgctgc ttggcaaata cgtcgaatcg ccagcgccgg
      781 agacctgcct gacgttcctc gccgaaaaag tcgatctgcg gctgcgcttt tttaacctgt
      841 tcaaaaagca tggtcttttg ctcaagctgg acccgctggc cgacaagcag cttccggggt
      901 ttgtcgaagc cgaagcgcgc cggctcaagg tcgatcttca gccggggacg gcagcccgac
      961 tggccgaaga aatcggcgcc gatctgggac agctcgccga cgcgctggag aggctgtgct
     1021 gttatgttcc ggttggtcag ccgattcgga ttgccgatgt cgaagaagtc gtcgtcacca
     1081 cgcgccagca ctcggtgttt gagctgatcg acgcggtggg tggcggcgat cgatcccagg
     1141 cactgacgct gctttccgac atgctgatgc agcgcgaacc ggcgctgaag ctgctggcgc
     1201 tgatcagtcg gcacgtcatg aaattgtggc agaccgcgga tctgctttcg caaggtcgcc
     1261 cgtcggcaag tgaaatcgcg ggagcgctcg gtgtgatgcc gtttgtcgcc ggaaagctgc
     1321 tcgatcaggc gcgccggctg aagctttcgc gggtgcaggc catgcacgaa gcgatttttc
     1381 agaccgatcg acagctcaag cagtccaagc tcgacgacga gcgaatcatg gaacagctga
     1441 ttttggggct gtgtgcaacg taagcgagcc gcttagggga agcggaccac gggcagccgc
     1501 gaatcgccgt cctgtccaca gcgacgggca gccatcacgc tgtccatatc agacggtgac
     1561 acttcgcgtg tcagcttggt gaaatcgatc tgcgtgcggg cgcacagcga caatagctcc
     1621 ggcagcaagt cgggatgcgg agccacggcg ataagctgcg cctgcatgtc gacgatgtgc
     1681 gcaagtgcag ccaggctcga cagtgcggcc ccggcctgcg gttcggcgac gagctgaccc
     1741 gcacttccat cgagcggctg cgacggctca tacaaaagga ccgtccccgc cggctcagcc
     1801 atttgcacgg cccgcgcacg cgccgccaag ctgcccaccg tttcgacgag aaccgccccg
     1861 gccaacgcca gtccttgcgc cgcggcacgc acggaaatct cccgtcgagc tgccgcagga
     1921 tcgagccgtt cttcatccga cggggacagc cgctcgacga caagtccgag cgaatcagcg
     1981 acaagcgcgg tcagatcagc ccgcagacct tgccccagca cgacgcagac cgtgcctgca
     2041 cagaccccgg cgcgcacaaa gcccgaatac ggggtcaaaa gtgcatccga aagcacggcg
     2101 tatggccaca gattttccgt cgctggactt gcatcgacaa acggcggcac aagcggcaaa
     2161 agcgcacgcg ctgggaccac ttcgcgcagg cagggacgga ccggacgatt tcgccgctgc
     2221 ggacaggccc gcaccgcccc acgacgacag gccggacact cgccgcaggg caaaagccgc
     2281 ggcacaagca ccgcctggcc gaccaaatgc tgcgccgcgc ccccggcttc ttcgacgacg
     2341 ccgaccgatt ctgcccccgg aaatgccccg agcgccgaac cataaatacg aactgccact
     2401 tcacccgctt gtaagctcac ggcggcggag gtttgcaaaa ttgtcccgcc gttggcaagt
     2461 gcggtcgccg ctgactgcga gcaaaaattt gcgccgacgc acgccgggcc gtgtaatcgg
     2521 cgcatgtacg aagcgtgctc gacctgcggt gagcgattca ttccgcagta tgcctaccaa
     2581 gttgcgacct tgcccgcagc gccgggtgag gccccgcgtc gtcagtattt ctgtcagctc
     2641 acctgtcggc gaaccgcgct tggcgaagtc acccatgcgg tccgtcgcgc ccgtcgcatg
     2701 gctattttga accaaaaggg cggcaccggc aaaacgacga cggcaatcaa cttggccgcg
     2761 ggacttgcag agcgcggttt cgagacgctg ttgattgata ccgacgccca aggcaacgtc
     2821 ggtgccagcc tgggggtcaa aggcgaccgc agcttgtatc atgtgattgt cgacggtgcc
     2881 gatcccgcgg aagtcgcggt gccggtgcgc ggacggctcg atgtgattac cgccgatgcg
     2941 acgctggctg cagcggaagt gtggctggcc cgtcgcgacc aagaccgcga ccgcgtgctg
     3001 tcgaatcgca tgggccaggc gcaccgtcgt tatcaatacg tgattttgga ctgcgcgccg
     3061 tcgctgtcac ttttgaatca aaacgcgctg acctatgccg acgaagtgct gattccggtt
     3121 tcgtgtgact atctttcgct cgtcggtgta aagcaggtgt taaagacgct caaagacatt
     3181 gagcgccacc tgggtcactc ggtgcgcatc gcaggtgtgc tgccgacgtt ttatgactcg
     3241 cgcatccgtc tggcgcggga agcgctggaa acgctgcgcg gtcacttccg cgaaaaactg
     3301 atggagccgg tgcgccgctc gacgcgactt gccgaagccc cgagccaccg gcagagcatt
     3361 ttcgaatacg acggagagtc gcccggtgcc gaagactatc gccggctcgt cgagcgcgtg
     3421 attggcaaag aacaagccac tgcgccgctt ggcacccgtc cccgcgaaat cgttcagccg
     3481 tcgatggaac cggcgaccct agagtaagga gacccgcagc atgagcctaa aaaacagtgc
     3541 cccgcgggcg ctccagaccg atccgctttc cgtcgacgac atcctggcaa cagggtttta
     3601 ccgtcccgaa cgagaggccg atcgagccga tccgcgcgaa gccgtccacg agctgcccgc
     3661 cggacaagtg cccgaaaaga agccgcgccc gacgcactac aaggtcgtgt gcatctcgct
     3721 ttacaacgaa gacattgaac ggctcgagat gctcgtgcgg gagctgaagc gtcgcggcta
     3781 cagcaaagcg aacaagtcgc tggtgatccg cgccgcgctc gaacagcttg atgtcgaaaa
     3841 agtgccgcgc ttccactaat accgccgagg gaaaagtgtc tacgcaaagg aagcttcacg
     3901 tcgctgtgct ctacggtggt cgctcgggcg agcacgaagt ctcgctccag tccgcagcgt
     3961 cggtactgcg caacctcgat ccggcgcgct ttgtcgcagt cccgatcgcg atcgacaaag
     4021 acggacgctg gcatccgggc gatgtgtcgc ggattgaaaa aggggcgcgc acgctgcctg
     4081 tattccgcga gcaaccgcaa gtggtgctgc cgccgaatcc gccccaaagt gcaggcgcgc
     4141 agcttgtgcc gctcgagaca acgacgctgg tgggtctggg actgccgctg ccgccgatcg
     4201 acgtggtgtt cccggtcctg cacggcacct actgcgaaga cggcacgctg caagggcttc
     4261 tggagctggc cgatgtgccc tacgtgggct gcggggtgct cgcgtcggcg attggcatgg
     4321 acaaagaagt cgccaagcgg ctcgcgggtc ttgcgggcat tccggtggtg ccctatctga
     4381 cggtgcacag tcggacgtat gcagcgcagg cgtcgacgct gcccgccgaa gtcggacacg
     4441 cgctcggtta tccgtgtttt gtcaaaccgt cgggctcggg atcgagcgtg ggcgtgcaca
     4501 aagtcaaaaa gcccgaagac ctcgatgcgg cggtgcgtga tgcgctgcgc tttgatcgca
     4561 aagtgctgat cgaaaaagcc atcgacgcgc gcgaaattga agtcgccgtg ctcgaaaaca
     4621 gcgatccgag cggcgaaccc ctgtgcagca ttgccggcga gatcaacccg cgccacgagt
     4681 tttattcgta tcaggccaag tatctcgacg aaaacggtgc ggcgctgctc gttccggccc
     4741 cgatttctcc cgcgcagctc gccgaggtca aaaagtgggc ggcgctcgcg ttcacgacgc
     4801 tcgaatgtga aggcctggca cgggtcgact tcttcctcga tcgcaagaca ggacaatttt
     4861 actttaacga ggtaaacacc ctgcccggct tcacgtcgat cagcatgtat ccgaagctgt
     4921 gggaggcgtc gggaatctcc tatgcggagc ttttgagtcg actggtcgat ctggcacttc
     4981 gtcggggcca agagaaaaaa gcgctgatcc acgactttgc cgcgatctag cgcgacggtc
     5041 tttttggccg cgcgatgcgt cgaccggcgc attccattga caaggtctct ggcgactcga
     5101 aaaatggcag cgaggtagat tggtgtcgcc atggcacaag cagagggact agccaagatg
     5161 gacgcgcaag gcagcgacaa agacggaggg tgtggccaag atgcatcagc aggcacgcca
     5221 aagcgaaggc ggactgccaa gagcgagcat ctgatcacgc tcaaagcgat ggtcgacggc
     5281 gccaagacaa cgccgcgggc cgttcggctt tacgaagcgg aaaaactgat ctccgcggcg
     5341 cagcgttcgt ctggcggtca tcggctgttc gacgaaggag agctggacaa gctccggctc
     5401 atcatcgatc tgcgcgcctg cggtttttcc atcgaggaaa ttcgcgaact cctggcagcg
     5461 cgtcagacga gcccatcgaa agagtcggcc atggcactgc aggctttgct cagtcggcac
     5521 atcgaaggac tgaagcgcaa gctcgcgctt atcacggagc tggggcgaga gttccagtgc
     5581 gccgtcgaga ccctcgatcg ctgcgcgacc tgcgacaatc ccgccggaaa aagtgcctgt
     5641 ctttcgtgcg aagtgctgac gagccgagct gcgccgcgct gcatgcgatt tatctgaccg
     5701 atccgtcgcc caacgaagcc gccgcgcgac aaaaagtcat tgcagcgggt tttcttcttc
     5761 gggaaccgtc acaaggtcaa caaaccttgg gtgggcacgc agcctgtcaa agtcgttcat
     5821 tcgatacatc gacgcacgca gtgagctgcc ttcacgcgcg ccatgatgcc aagtgtcacg
     5881 ccgtgccgct tcgagcaggc gctgcaccgc aagtccggtt tctccgacgg caagacggta
     5941 ataaatcacc gatagatcgg tgccatcccc ggtaagctcc gaatcgggaa tcttggcaaa
     6001 ctcttggcgc gcttcggcca gctcaccacg ttccgccagc atcgccgcgt tgccgatctg
     6061 gatctcgcgg ttgaagcggc tgcgcagccc gagcgcctcg cgataggcgg caagcacccg
     6121 atccgcaaac gccacgtcga tctgcccggt aaacccacgc accgccaaaa acacgttgtg
     6181 cagaagcagc gcccgcagct gtatcgccgc cgcctgttcc ccaccgcgat cggcccgcag
     6241 cttgagctgt tcgtcgatca gcgacagctg ctgaacctgg ccttcgacgt aagcaagccg
     6301 aacaaaatgt cgccgcggca aaggcccgcg caaaggtccg tgcaaaagtt cgcggtacgc
     6361 cagtgctcgt gcgcgccgaa tcagcgaaag cccgtcgcgc aggttgtaac ggcgaagctc
     6421 ggccattccg cgggcatagt tttgccgaat ttcaccgtcg agcccagctt ccaaaagcgc
     6481 gctctgcgtc gactgcacaa cctcgcaggc tcggtccgat ccaaccaccg tcgacgatgc
     6541 tgccagcgca cagccaagta gaagcgcagg tccgagcagc atcgcttagt ccttcacgcc
     6601 aatccgacgg tgccgcaggg ccggcagatc agtccgaatt ttctgtagcg attcaaaatc
     6661 cagctcggcc accgcgacgc cttctccgtc gggacattcg cacaacaccg tcccccacgg
     6721 atcgactatc aagctgtgtc cataggtaaa ccgcgacgga ctgtgccgac cctgctgcgc
     6781 cgccgcgagg acgaagcact ggttttcaat cgcgcgcgcc cgcaaaagaa cgtgccagtg
     6841 atcttttccg gtatgcagcg taaacgccgc cgggatcgac aacattttcg cagctgccgc
     6901 cgaaagccga cgatatagct cgggaaaacg cacgtcgtaa cagaccgaaa gccccagtcc
     6961 tccccacggc gtttccgaca cgatcacctg atcgacagca ccatattcca ccgtcgctga
     7021 ttcacatagg ctcgtcgctg catggaaaaa atggacatca aacaggtgga tcttgcgata
     7081 caccgccgcg atgtttccgt cgggcaaaag gtgaacgctg ctgttaaaca cccgcgatgg
     7141 ggtttgcccc acttcaggca gtccaccgag caccaagtgc accgaaagcg accgcgccag
     7201 cgcttgcatc cgaccgagga tctgcccgtg accgtgcagc gtcccgtctg attttgccgc
     7261 gatcgtcacc gcatgactga acttgtcttt ttcatccgac gaaagcaggg caaaattttc
     7321 cggcagacag acaagctcgg caccccgtcg ctgcgctttc tggcaaagcg cttcgacttg
     7381 ggcaagattg cgctcaacat cagcagtcga gcaaagctga accactgcgg cttttaggac
     7441 cgacatctct cgcagtatac tgcttgccga agaaccatcg tctttgccga tgctggaact
     7501 taacaatcgc gctcgcaaaa ttctctcggc cattacccag gaatatcttt cgactgggga
     7561 agccgtcggt tcgcgcacgg tgacacggcg ctacggcgtc gatctgtcgc cggcgagtgt
     7621 ccgcaacgtg atggccgacc tcgaagaact cgggctgatt cggcagccgc atgcttcggc
     7681 gggacgggtt ccgacggact tgggtctgcg cttttttgtc gattcgcttt tgaaaatccg
     7741 ccagctttca tcccgtgacc gtgaagaaat ggccggacac tacaacttct cgacggggga
     7801 agtggatgca gtgctgcgcg aggccgggca agtgctgtcc gatctgtcga cgcacacggt
     7861 tgtggtgatg acgccgcgcg tcgatctcga cgtgctcaaa cacatcgagt ttgtccgcgt
     7921 caaagacaac gcggtggtcg cggtgctggt caacacgtcg gggcgagtgc ttaacaaact
     7981 ggtgacgctc gacgagccga ttgcgcctga cttgctcgat cgcatcacca attatctgaa
     8041 ctcatttctc gacggaaaaa ccctgtccga agcgcgctcg ctgatcgaaa aagagctggc
     8101 caaagagcgg acccgctacg acgcacaagc caagctggca ctgcggttgt cggcgcaagc
     8161 gctgccggcg gaacctccgt cggtcatcgt atcgggacaa gccaacttgc tgtcgcatgt
     8221 gttaagcgag gaagcggcac cgacgcccga gcggctcaaa gcgctgtttc gcacgctcga
     8281 agatcaccgg cagcttctgc gcctgcttga acagaccgaa gccgcgcacg gaattcaggt
     8341 ttttattggt gccgaaacgt ccgttcctga gttttcggac aaaacggtgg tcgccgcaag
     8401 ctatggtcca aacgaaaagc ccgtcggagc actcggcgtc atcggcccaa ctcgcatgaa
     8461 ctactcgcgg gttattgcgc tggtcgactt tactgcgcaa cttcttagcg gtgtcatcgc
     8521 tcaaaatcgc taacccatgc cagcttgcgg cccgatgacg cggcagttgc cgtcgaaagc
     8581 tgaccaaaaa agtcgtcaag gagagacacg aacatggcca ccgaagaagc gcagtcgtca
     8641 aacgcccccg agtcgtcggg acaagcccag tcgggacagc caccttctgc ggcctcgccc
     8701 gaggctgatc tgaaggcgca agtcgacaag ctctcggagg aaaagcgcga tctgaacgat
     8761 cggctgctcc gtctcgctgc cgagttcgaa aactacaagc gccgggcccg caaagagcag
     8821 gaagacgccg ccgcgcgcgg tctggagagc ttggtgcgcg agcttctgcc ggcaatggac
     8881 aacttcgatc gggcgctttc cgcgagcaaa aatccgtcga ccacggtcac tgcgatcgtc
     8941 gaaggcgtgc agatggtgca gcggagcttc tttgccgcac tcgaaaagtc gcagataagg
     9001 ccgttcgagg cagaaggcaa gccgtttgac ccgaacttcc acgaagcgat cgggcaggta
     9061 gaaagcgcga cgctgccgcc aggccacgtc ggcaacgtgt tccagcgggg ttacatgatc
     9121 ggcagtcgac tgctccgtcc ggcgctggtg caagtgacgc gcaaaagtga gcaagcgccc
     9181 gaaacgcccc cctcgacgcc cggccaggca aactgaggcg agaattggcc gacgcaaagc
     9241 gcgattatta cgaagtgctc ggtctgccgc gggatgcaga accgacggag ataaagcgag
     9301 cctatcgtgc gctggcgctg cgctatcacc ccgatcaaaa ccagaacgac aagcacgccg
     9361 aggacaagtt caaagaagtg tccgaggcgt acaccgtgct gtctgacccg gaaaagcgcg
     9421 cccgctacga taggctcggg catcttggac tatcgcgcga tgtgtcgggc atcgatccac
     9481 tgtccgtcga tttggaatcg atgaagtcgc tgtttagcaa cctgttcggc gacctcatcg
     9541 gcggcaaaaa aggcaaaggg cgcgatcttc gctacaacct cgatatctcg ctcagcgagg
     9601 ccgcacacgg ggtactaaaa accatccgct ttccggtccg cgccgactgc ccaagctgcc
     9661 atggcagcgg cgctcgcggc ggcgacgcag gactgcgaac ctgcaccacc tgcgctggca
     9721 aaggcgaagt aaagcccggc aaaggcctgt tttcgctgag ccgtccctgc accgtctgcc
     9781 aaggcgtcgg caaagtcgtc gtcgatccgt gcagcgaatg ccgcggcgct ggacttaccg
     9841 aacagcagcg cgaatacacc gtcagcattc cgcccggcac cgaggatgga tcggctcgtc
     9901 gcatttccgg ccaaggcgaa ccgggtcgac gcggcggcgg cggcgctggc gacctaaacg
     9961 tcaccgtcaa agtcgaaccg caccccatcc tccgacgaga aggtgcgctg ttgttgtgcg
    10021 atttgccgat cacactgacc caagcggcga ttggctgtgc cctggatgtg ccgacgctgg
    10081 aaggccgcgt cgaaatgaaa attcccgccg gcacccagtc cggcaccgtc tttcgtttgc
    10141 gcagtcgcgg ttttccgacg tcggtgggcg cgacgagccg aggcgatctg cacgtcaaag
    10201 tctttatcga gacaccaggg aacctcagcg aagcgcagcg cgccctgctc cgccacatcg
    10261 aagacaagct cggtggcgcc gactacccgc gcatccgcaa ataccaagac gacctaaaac
    10321 acctgtacgg accggcggac accacgacca ctcccatcac caagtccgac ggcaaacccg
    10381 ctaccaagcc caacaacaag cccgtcagct aagcgacacg gctgcgccaa aaagtcgggc
    10441 cgctgtcagc ccgctttgct atacccagcc gctgtgaacg aaacgtctca acgccgcgta
    10501 ttttggaccg gctgtccgcc ggaagcttgt gtccgccaga ccggcaggca gcgcgcgtcg
    10561 agcggatcga ttcgctttgt cccgcaccac tgtcagggct gacgcggccc aagccgccgc
    10621 cacatccgcc ggcgccgcgt tggctttccc attttccgac gaaaaaaacc aaggagcaga
    10681 cccatggcca tcgagaaaaa agacgacgca aagaccccaa gccgcagcga gcgccttgcc
    10741 gctctcgacg cagtaaccag caacatcgaa aagcagtttg gcaaaggatc gatcatgcgc
    10801 ctgggcgaag gcatggcgct gccggaagtc gtggtgatgc cgacgggttc gctcgcgctc
    10861 gacatcgcgc tcggctgcgg cggtctgccc cgtggacgca tcgtcgagat ttttggccca
    10921 gagtcatccg gcaagaccac gatggcgctg tcggtcctcg cagaatgcca aaagcgcggc
    10981 ggcatggcag cgtttgtcga tgctgaacac gcactcgaca tcgtctatgc caaaaaactc
    11041 ggggtgcgta ccgacgacct gctcgtttcg cagcccgaca acggcgaaca agcgctggag
    11101 attgccgaca tgctcgtccg cagcagcgcc gtcgacgtgg tggtcatcga ttcagtggcc
    11161 gcgctcgtgc ccaaagccga aatcgaaggc gaaatgggtg atcagcacgt cggtcttcag
    11221 gcccgcctga tgtcgcaggc gctgcgcaag ctgaccggca gcatcaacaa gagcaacacg
    11281 ctcgtcatct tcatcaacca gatccgcatg aaaataggcg tgatgttcgg cagccccgaa
    11341 acgacgacgg gcggcaacgc gctcaagttc tacgcttcgc agcggcttga tatccgtcgc
    11401 gtcggcgcac tcaagcaagg cgaaaaagtc gtcggcaacc gcacccgcgt caaggtcgtc
    11461 aaaaacaagc tcgcgccgcc attccgtgaa gtcgagttcg acctgatgta cggcgaaggc
    11521 atcagccatg aaggcgacct cgtcgacctc ggttccgagg caggcatcat cgaaaagtcg
    11581 ggcgcgtggt ttgcctacgg cggcgaacga atcggccagg gccgcgaaaa cgccaaagag
    11641 tacctaaaga cccatcccga aatggccgac gcgatcgaag cggccatctt ggaaaagcac
    11701 gggatcaagc gacagggcat cgacccgtcg agcaccgctg cggcagccag caccgcagaa
    11761 catgtcgccc ccccgactgg gcgcaaggca aaagccgcga gctaaccgcg cctagtcgac
    11821 gatttcggga cgaccgctcg gcttggtctt gccggttggc ttgccgccgg caactggggt
    11881 ggtttttccc ggtccagcct ttgtggttcc cgtcggtggg attgggtttt tgaccgcgac
    11941 cgacttgccc gctggactcg tgccagcagg cacttttccg ctcggtccgg tcttcccagc
    12001 cgcaggcccg gtcgacttgg tagcaccgcc atttttcccc gcgggtggcg tcggcgctgg
    12061 cttgctgcca gtcgctggct tcggcggatc gaccggttca agcggcacat tcaagctgac
    12121 atcgctgccg acattggcgt cgacggtttg gtctttgtag ttaggtcgcc ggatgatgag
    12181 cttgagcggc tctttgccgc gcggccagtc tttcttcatc ggcgtaaggc ctatcgaccg
    12241 ccccgagctt gccagcagca catcggcacc tgccggcgtg ctttcgaccg acaacatcac
    12301 attgcgacgc tcgattttgg tttgcaccac tgccggctgg ttgccctcac gctcgtcgcg
    12361 caaaaaacgc accgtgatcg ccacgaccag cgacgacacc acgatggtcg ctgccgcaac
    12421 caccgcaatc cagcgtcgcc gccgcgctgg ggtcatcgaa gcgctgctct gccccgtcgc
    12481 tccagcgagc gtcgattgtc gaagccccga attggaatcc ttgtccgctt tttctttggc
    12541 gactttgagc cgattccccg tcggggtatc gtcatcaccc ggcgaagact gggctgccgg
    12601 caccatcgcc cccgaagcgg cctgcggcga cggtgaagcc tgacccggct ggacaaagcc
    12661 cggaatcggt atgttggccg acgacagctg aaagcccgga ggcatccgca caatcgcatg
    12721 cgccgtcgtc ggataagcca cggccaagcc ttccagttca ccggcaacct ggcgcatcgt
    12781 cggacgatcc gccttgtttt tgatcaagag ccgatgcacc acctgtccca ggttgaccgg
    12841 cacatgcggc gcagcctctc gaatcggcgg cggttcgtcg taaatttgct tggccaggat
    12901 ctcgccttgt gccccggtaa acggcggctt gccgacgagc atttcgtaca tgatgacgcc
    12961 gagcgagtac acgtcggact tgtcatcgac attgccggca ccacgacact gctcgggggc
    13021 catgtacttt ggcgtgccca tcacaacatc ggctgccgtc tgcgccgcgc cttggtttag
    13081 ctcggcggcg accttggcga tgccaaagtc gagcagcttg acccgaatcc gtcccggcgc
    13141 ttccgggtcc ggatcggcaa tcaacatgat gttgtcaggc ttcaaatctc ggtgaatcac
    13201 gcccttttcg tgcgccgccg caagcgctgc cgcaatctgc cgcgaaattc gcaccacttc
    13261 gggcaccgcg agcttgccgc cgttcgcttt gagccgctgc cccagcgtgt cgcccttgag
    13321 aaactccatg accagatagg cgacgcccga cggcaactgc ccgtagtcgg atatctggac
    13381 gatgccggga tggtcgacga tgttgaccgc ccgcgcctcg ttgaaaaagc gctgcgcgat
    13441 ctccggttct tgggcgtaac gaccatgcag aaccttgatc gccacgcgcc gctcaatcgg
    13501 ctcttggatg gcctcataaa cggcccccat gccacccttg ccgattaggc gtacaaggcg
    13561 gtacgggcct atcttgtctc gcggcagacc tgtagacact acaggcgcat cttagcactt
    13621 tgggctttgc tacatgaaga gagttccgcg acttttccgg ggaaaccgcg cttgggcaag
    13681 cgggctactt tttgcggttt tccggctcga ccgcggaaag cgatttgacc gcagaaagcc
    13741 cgacgggaat cggcatgatt tttagatccg gtcggccccg gcccgcatcg ccccggtagc
    13801 gcacaaaata gacgccagcg gtatgcgcgc tcagcgttga acactgcggt gccactttgc
    13861 gtagctggcc cccgtcgtca gatggatcgt cggtcacagg cgccgcaacc gcaggaagct
    13921 gcaaacaggc gcggtggctg ccggtgcccg ccgcatagcg gaaataccgc ccgcgcagaa
    13981 gccgaacttt ttcgctgtcg tcgacgacca tccacattcc atcccctgcg tcgtcggcat
    14041 cacgataaaa gtaaatgatt gtcgaaccgc tcgcctgggc aaaaggatgc ggatcgggaa
    14101 ccgctatcgg tggctggcca aacaggcgct tttctgcctg ccgcgccgcc ggggccgagc
    14161 tgctttgcag cgccgcgagc acggacggac gaccggtatc gtattcacca tagtgaaccc
    14221 acgccgcaat cgccgcttct tgcaccgcat cgctgtcatc ggccagtgcc gctttgagcg
    14281 cgctgtcgtt gccttcggct tcccaggtct tgaccttggc gacccgcatc gacgcgcagc
    14341 cctgcaaaag cagtaaaagt ccgacgcata gaccttggat tcccagtcgc ttgcgcatcg
    14401 ttttttcccc tcgcgcgctg ctttttttca tcggctcccc gcttcacgat cgaacgttgc
    14461 ggtttggctg cccactttct gaaacagcgc gacgttgtgt tcgactgtct cgagatcgtg
    14521 aaaagcgcgc agctttgcat cttcccactc tcggtccagc tcggaaagcc cccacttgcc
    14581 gtcaagccca cgcgcgagca aaaacggctt gccagccgcg caaccacagt gcacgacatc
    14641 gccgacgacc ggaaccgtgc gcatgtcttc agcgacgcgg gcccgaaaat cagcggcata
    14701 gcgctcacca tacagcgaca aaaacagctc gcgtccactg tctgccgacg atgcaaacag
    14761 ccgcccgagc gccgcctcac gctgggccgc tggatagctt gcacgaatct gcgtcgccat
    14821 ctgtcggtgc agcttgttca ccgtcgacac cgaccactgg gtgttttgca aaagcacgtc
    14881 gtagaccgcg atgccgtcgg cttcccagat cgcatcgcgc agggcaagac acgccgattc
    14941 agcggttcga cgctggccgc agcccaccaa caacagcaaa agaccaatcc acagccggcc
    15001 ccagaaaccg gccccgtggc gaagaagcca agacgcgcag gcggtcatgc gctcggcttg
    15061 tctccgtcct tggtcgactc ggctgctttt tcgacgagct cctctttgtt ggcttcgctc
    15121 accgaccgct tttgcgcaag ctcttgccgt cgcaggttct gcatgtggag gaccagccgc
    15181 atctcgatgt aaagcccgac gatcagccag atcagcggaa agcccggcag ttcgaagcca
    15241 cgcactttgc cgtaggtcag cagtccgccg accatcgcta tcagcgcggc tcccgtcgga
    15301 atcgccagcg aaaagacccg ctgaaaacca agctggagtc cccccaaaag gagcgccatc
    15361 ccgacgacca gattgcccgg agcgagcatg agcccaaacg gctgcacgcg cacgatggca
    15421 aacacgccca tcatgcacat catcacgacc aagtacgaag tttgcgagcg cacgaaagcc
    15481 cagctaacac gtctttggtt tgggcgccat ttcgcccatc attttcgccc cgctttcgcc
    15541 gtccatttcg ggagttttcc gtcgcccacg ttttttgatc ttgccgctca atggccgcct
    15601 ttggtattga aggcacctcc taaagcaagc cgattgctgt tttttggcca tcacgctctg
    15661 cgtcgcgcct tctgcctgta ggtgcacttg aatcaagtca ttcggtactg ggtgaggctt
    15721 atccacagct gtggataagt tgtggattgt ttaagcatgt tgacggatct gcccctcgat
    15781 agctcagcat cgggaaatcc cccctctccg gcagggacaa cgatgctgtg ggatgcggcg
    15841 atggacattt tgtcccgcaa gctgtcggcg cagaacttcg acctgtggtt tcggcccatc
    15901 cgctgccagc gcattgacgg ggcccacatc cacctgatcg ccccgaatca gttcatcaaa
    15961 gagtggtttg aaacccacta tcaaaaagtc gtcctcgacg tgattggcga atcgagcgat
    16021 cgccgcttca ccatccactg gggaattgac gacggggcac atgaaagcga gcgaggacgc
    16081 ggggacgaaa ccgccgccca gccgaccgtg cccgaaaaac cggcggcgaa aaagcccagc
    16141 cccagcgtcg atacggcacg cagcgcggca gcgcgacgca gtggcacaaa cggcaaagcc
    16201 gcatcacaga gcgatggcgc cgaacccgga ccggagctgc tcgcgcggta cgtgtttgat
    16261 tcgttcgtca tcggtccgtc gaatcagctt gccgcagccg caagccgtgc cgtcgctgac
    16321 caccccggca gcaagtacaa tccgctgttc atctatggcg gtgtcgggct cggcaaaacg
    16381 caccttctgc acgcgatcgg tcacaagatt caggccgacc accctgggtg gcgaatcctg
    16441 tacctgaaga ccgagacgtt catgaacgag tacataaacc aagtgcgctc gggccgcatc
    16501 gaggagtttc gcgccaaata ccgcgacacc accgacgtgc tgctgatgga cgacatccag
    16561 tatttgggcg gcaaagagcg gacccaggac gaatttttcc acacgttcaa cgcgctgttt
    16621 gaatcgcacc ggcagatcgt cgtcaccgcg gacaaatacc cgcacgagat ccccgatctg
    16681 gaagagcgca ttcgatcgcg ctttcagtgg ggactcatcg ctgacatcca ggcgcccgag
    16741 ctggaaacgc gggtcgcgat tttgcaaaag aaagcagagc tggatcagat cgcgctgtcc
    16801 gacgaagtgg cgctgttttt ggcgacccac a
//
LOCUS       DAORGN010000104        16520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1430_length_16520_cov_5.903492, whole genome shotgun sequence.
ACCESSION   DAORGN010000104 DAORGN010000000
VERSION     DAORGN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_16520_cov_5.903492"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /gene="dnaE"
                     /locus_tag="PKL17_12450"
     CDS             <1..2063
                     /gene="dnaE"
                     /locus_tag="PKL17_12450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNK45591.1"
                     /translation="EYKELVEIATALEGLHRNAGMHAAGIVIAEHPLWEHVPCYRGQD
                     GEIVTQFAKEEVEEAGLVKFDFLGLKTLTVIDIAVRMIEKVKPDFDLRRIPLDDSGVY
                     RMISDGDTTGVFQLESSGFKELLKKLKPDCFEDIIAAGALYRPGPLEGGMVDDFINRK
                     HGRTKIVYHHPAMEPILKETYGVIVYQEQVMQISRALAGYSLGGADLLRRAMGKKKKE
                     VMEKEKAKFLDGATKTGVDTKIADEVFDLMAAFAGYGFNKSHSAAYGLVTYQTAFLKK
                     HYPAEFWAALLTCDKDDTDKVARYVAEVRDSGIPVLPPCVNQSEMDFSVIRLSEKHPK
                     SGVPIERQAIRFGMSAIRGVGEGAVEVILAARKEKGNFASLFNLCERVDLRRTNKKVM
                     EALIKAGAMDAIVPGRAQAMAAIDSAVERAQKAQREKESGQTSLFGLLSGGGAKSGSD
                     FGPPPPYPQVADWIPKQKLALEKEALGFYISGHPVDRYAPELRRARAVAVAELADDNF
                     EIPRGRDRADILAGGVVSDFRERILKSGTGKMASFRLEDPTGGIEVVCFSKAYAEYET
                     VLKADEPILVIGQLMSDGDGENPQRKINLREAQLLSQIRMEKTQKLTLSVVADDLTEA
                     KVTSLKDALLAHPGPVQISILLQKPGRWRVEAKLPSDIKIQPSDELLTRLGTLCGDDA
                     VQLG"
     gene            complement(2076..3137)
                     /locus_tag="PKL17_12455"
     CDS             complement(2076..3137)
                     /locus_tag="PKL17_12455"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNK45592.1"
                     /translation="MDDALSALKTEFKNRGWNQRHVGQVIAELVVHVCLALGGIAIFV
                     FCEPWWLRAIGMLISTYGSVGVGTNTHTATHYAASPHRWVNEVLTYFGYPFFLMLGAT
                     FWRHRHITLHHANPNVMGIDGDADFGPFFASTDEEVAQGSAWFLASQRYVFPCMAWLN
                     SYLRQIGSWRHLFAILPDKRKRRLAHVADAAVMSLHIVVWFVIPSLYFPVLSVIGFNL
                     LRIGLLGYPLYCVLAPGHYPEEALCIAKGDWRKDFVLLQTATTINFSTGPIGAFLCSG
                     LQYQIEHHLFPGYSHTHYAKMAPLVKEFCIQNGYPYRTFGWVDAVMKTFTIFSKPKHV
                     YPDVDALRQAVKEQLGTAS"
     gene            complement(3256..4080)
                     /locus_tag="PKL17_12460"
     CDS             complement(3256..4080)
                     /locus_tag="PKL17_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45593.1"
                     /translation="MSSCAKSVFRRVSALSVLWLLPLLLSVASCDLLDSLAQNAENPP
                     TVQASALQLTKRPSVTQLAAYYCPTVITDSTLRLLCTVTLGSPPPASQLTFNFGIHLL
                     ITNPNNVPVPALDVLLALRLFDGQQAEALGAICLSMCGSADPTCTGQPKPGACTSSQT
                     DIRTLNDFQARIPQLITDLVTGKAEQELRKSTIAAAGNVNLDLVFVLGLDQALSVFQK
                     VAVSFVTDLLNGKNPTLSVPVEAEGSVFFELQPVGRFGVGYGPLRTTWNIDSTILN"
     gene            4199..5233
                     /locus_tag="PKL17_12465"
     CDS             4199..5233
                     /locus_tag="PKL17_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45594.1"
                     /translation="MSSMFVNSPPPLDRERPHLLAIPTGFSLAQAIRISPIGLLPPLL
                     YDPVQPAMQAVIPIAHGPSAAPRPLQEVLIRGVPGHDALLIYGLGEGPHVEPVLDRVR
                     WMLGLDDDLFLFYHLCSRDRDLAWVAEQHAAHVLRSPTVFEDLVKTLVVTQKAKQAHA
                     ICQTLCDAFGSPTMLSRLAFPTEQQLALATEDMLAKELGLGSLGTMIHRLARRCADGQ
                     PHPETLRRHRPSISQALADDDEELFDDLVVEELHWQERVAELLLTLPGFGVRAVPKML
                     RLLGCFDLIELHHSAMQTFAKRYPKRRKTAESETAEEMTDRLLKRLDGFAIYRSLAQS
                     ILLLPPERSA"
     gene            complement(5194..6537)
                     /locus_tag="PKL17_12470"
     CDS             complement(5194..6537)
                     /locus_tag="PKL17_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNK45595.1"
                     /translation="MKLCAQRHGALALSSVLLLSLACQLERRSAAEPAILAVRVAADA
                     GSVDASSAAENPSPSDAGIGPISDGGSGLLPAPPPPLSLPGGTWMLPRGDLHRTGRSP
                     FVLPDKRPQVVWKFATKGKVTAAPILSPDGTVVFGSHDYSIYGLHPDGTLRWKRATLD
                     MVFSAPVVTPDGLVLVGSDDDKLYSLNLSDGSVRWTAAPGRCARSVGVGPEASRCDIE
                     GVTPAPDGSLYVGGDGITALSTDGKVRWHYAVDKKHCGSSPSLGFDGSLYAVCQDLLI
                     SLRPDGSKRWEIALAIDLAEAPAIGPDGTAYLGAEDRRIYAVSKDGVIKFSVPTGAPV
                     RAAVALRSDGTILAGSYDGQLYAVRSDGTLLWKFQTGDAIQSAPIIDRKGRVLFGSRD
                     DRLYALTVDGQLLWQVPFDADVDGSPLLAEDEAILVGTDDRHLYALRSGGKSKID"
     gene            complement(6551..9832)
                     /locus_tag="PKL17_12475"
     CDS             complement(6551..9832)
                     /locus_tag="PKL17_12475"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNK45596.1"
                     /translation="MPSNSAVADVAPGPFRPRPQPSIIWREVKPVDPVLQLARPAAAP
                     MSLTSSDGAGLELVSLEAKAVVEDPLTFTELHLTFRNPEPRVREGQFEIVLPPGAAIS
                     RFAMRQGNDWQEGEVVELQAARRAYEDFLHRRQDPALLEKQAGNSFRARVFPIPPSAT
                     KELIVSYSAERQNAADPFRIYLRGLPKLSKLSIRAIVAKNQDGGLTSSLGGKSLAHQT
                     VTVEKDNFTPDVDFEVGVPVPTANDGRAVGLVHQNLLVARITPQTVLGKVGIEPIEGL
                     VVLFDTSASRALGYKAQVDLLGSLCAELSRQGEGRTPLRILAFDQTTEEIYSGSVGDV
                     GPGVLEKLRARRALGASDLAQALRVVAQSKSSFRRVLLMSDGVVTAGPTEAPQLEKSV
                     QALKGIGIARLDAVTVGGLRDESLLRKLTTMGLASDGVVLDGDWPTQALALRLRRAVR
                     SGIEVSVPGSKWVWPLRLDGVQPGDQVLVYADVPDQQAAQVVLRGRENQTETIGVATV
                     EVPRPLLERAWVGARIARLSHQRDTMAASDPDLSEGLKHQIIDLSTKFRVMCDFTALL
                     VLETESDYARFHIDRRALTDILGVGMDGIEVRSRKALAQAIPPPPPPPPQNPSWMSRP
                     MPKPAPPSATRGPRGMAAESAASASVADKRAVAADSAPDGAMLSMPASAPGGAATGAP
                     APIVAEGVAAAPARAMRESRRSKDDAADGMIGGSGGAPAAAPPPPPPPPRPEPRFDRA
                     EKAKEEERPRAQPVERRAMAMRPVPPSSVLRPVNQDDDASQRPFVNPYEGKLADVMNL
                     LKAGRAGEALQRAQAWRDSDPGDVLALIGLGEVFESQQQRRLAARAYGSIIDLFPGRA
                     DLRRFAGARLEKLSEGLDLAADTYAKAAVNRPDHPSSHRMLAYALLKLGKPAEAFEAI
                     VSGAARSYPSGRFAGVPRILAEDVGLIAAAWLRKEPQRREEIERRLQGLGAQLASGPS
                     LRFVLSWETDANDVDFHILDGRGGHAYFSNPSLPSGGELYADVTTGYGPECFSIFAPS
                     GQRAYPYKLQAHYYSRGPMGYGMGKLQIIEHDGKGGLRFTERPFVIMVDGAFVDLGEV
                     RGPLP"
     gene            10024..10485
                     /locus_tag="PKL17_12480"
     CDS             10024..10485
                     /locus_tag="PKL17_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45597.1"
                     /translation="MNARFCSGILVAAACWALGCTPPPTPPIQFQAGNLAREPMDALL
                     ESAKKIGMYPASVTPGLIETRWEETAIFAEPLLEKKTKLVRRYLLRIEKRAFGHQVTI
                     SVQARRCIPSTLRFGETDVEGQCAEVPTLPPPLVTELNRTADRIEQQLAIP"
     gene            10516..10737
                     /locus_tag="PKL17_12485"
     CDS             10516..10737
                     /locus_tag="PKL17_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45598.1"
                     /translation="MDTNERPAPVDVRIGGTAARLLLSLMEQLQTEDGGGVITRALGL
                     LDLALAARRQGKSFCLVDPKTGDSQEIAF"
     gene            10821..11423
                     /locus_tag="PKL17_12490"
     CDS             10821..11423
                     /locus_tag="PKL17_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45599.1"
                     /translation="MQLDPAIQNPPAEIAPAQTTENAGSAASDGSLPKKTRGQLLLSV
                     GLLAGGTTWAGDALGTGSLLLGLRLYRSIALVVQGRLGYGRIDQRQLTAILIGLMGGG
                     YLANKVYGRLSVSFVHQHEESIAAVAEQPGGALLGIGTGIRHRAGVQGNLGVDFIVYR
                     QPKYELSLGPEVAISYLTYSTGPSIYGFAGLSAGSAFHLF"
     gene            11434..11805
                     /locus_tag="PKL17_12495"
     CDS             11434..11805
                     /locus_tag="PKL17_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45600.1"
                     /translation="MLHPFFRFSVAGLVLLATSSCGLFYSDIDSYPCPPAGTQLRYQN
                     FGAAFVDSYCQSCHSSQSIDRQGAPGEFIFDTVEQIQRHRARIFVRSAADNDSMPPGP
                     NDPSKEQRKQLAEWLACGAPN"
     gene            11834..12514
                     /locus_tag="PKL17_12500"
     CDS             11834..12514
                     /locus_tag="PKL17_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HNK45601.1"
                     /translation="MPPMKPPIKLPTLPYRKPVEGRDLWILDNALPNVDEVRARCLAK
                     TDWTYGFPYRDEGWPGMRSIPALLPDELAPIEDWVRKVTGYKRLWQNPTPEGMTLNHN
                     CVQVVGVDEADARPHTDSRPNCRLAAVIYLTPGLSPDCGTSFYRQRLPGGKLGGNHVP
                     PPHANLQAALGTRFVPPNSFIEDARVDYKYNRLVMYHADLIHSATRYVGKSLETKRMA
                     AVFFWLAQ"
     gene            complement(12536..15421)
                     /locus_tag="PKL17_12505"
     CDS             complement(12536..15421)
                     /locus_tag="PKL17_12505"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNK45602.1"
                     /translation="MSPFPTRRFHAGGVVPLGQAFYVERPEDDRLVSELLSGRLCYIL
                     GTRQIGKSSLRVRAMSELRRRGAKAASLNLQGFGFPPEAEWYRGLIQRTAKELELPFS
                     ASDFFSRHSQSGPVHLWTSFFHEEVLPHIDAPVVIFFDEIDTVLSPDFRFSTDAFFAS
                     LRSLYEEVRGSFSKKPRLTFCLIGVATPEELIKDPVRSPFNLDNTRLYLDDFTAREAE
                     VLLPGLSVLPGNSADWLAHVLSWTDGHPYMTQRVCQYLVDKPQTDNGNPADSVKDAVA
                     KLFLSGHKDPNLSFAEAQFQHVSDHHDRHSAVTLYRRIFVGDRVIYEPQDRLHSLLLL
                     SGMVKLRQDGTERRLIARNRIFREVFGSKWIQERDRSTPDWLTDKLFAYWEHGRGANY
                     SLSRIEIERALDTIPKSGQSLPPDLAEFIGRSQQVRDREERRQRTIFALAVVGLLSLL
                     PFAAYQTYRRVLDKKDADQKAAVRDSQIKLLQEYRSYAEQQIAKLTARIESLEGEVGE
                     LYSALRDASQQLLRKEKELGKLRLQKHETELRLRDLRREKSEVERRLRHREQEKNAAE
                     RESQEHEKVRLRIDERLRALEPSPSLRVGPPLAKEALQLRDVVTELAGKIGHLSQPES
                     LKLRQAVGHMGTPRLPFLRVSEPGLHHLMLCPFARSAEGKPFLLAASQSGVSLWDGQG
                     QPLSAEFPLPDKAVVHSLAAKYRCSQVMISVDNRVLTFTEQGQLLNQLRNPQPAQVLS
                     ALVSPDDDPPQGFPLVIGMGARAEVVYMRQGGHKVGSQLLHVDEVTAIELSQNGQLVM
                     TAAGKRVLLWQAVSGERLRNLHEHAARVRTIALSHAAGDSVAVSGADDGSVAVFGTTT
                     GELMQNLRGHTGSIRSAEFSLDGHYIVTSSADRSVRIFSRQDGSLVFALSDLPYPPLS
                     AELSSDGKQLVVLLAHGEARLYSISDVEFLHAACHKLRPIVSTPACG"
     gene            complement(15467..>16520)
                     /locus_tag="PKL17_12510"
     CDS             complement(15467..>16520)
                     /locus_tag="PKL17_12510"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNK45603.1"
                     /translation="SDAGLTAISLEHYVPRERLERPLRRYLRGETHNPVVLWGPAFAG
                     KKTLLRHVLASVRSDEAKRGRKPLEVLLDLANFKLDHRDHLDSVLRGIGLRLARAVAQ
                     RSGEKDYAQWVKTRFEQHDHIERLNWLLEEDIFPRLSSGLSLILLSGNLLLHSPLGAE
                     VFGMLRHWADCTQQALHLSGDGAADSAGWLSLRLVTVISTLESAQDVIHGSQFLNHAE
                     RIEVDDFTREEVVALIRGYDRLPGADVATDALLQIVGGQPNLLRRILDDAARLGVLHL
                     DREHLIRVCQHHLESIRQWIRRSPELDDSVQRLLRGGPLTMAERQRLMASGLTRQTQT
                     GIKLRYPLYEDYLRGL"
BASE COUNT         3538 a         5121 c         4673 g         3188 t
ORIGIN      
        1 cggagtacaa agagctggtc gaaatcgcga ccgcgctcga aggactgcac cgcaacgccg
       61 gcatgcatgc ggcaggaatc gtcatcgccg aacacccgct gtgggaacac gttccctgtt
      121 atcgcggaca agatggcgaa atcgtcaccc agtttgccaa ggaagaagtc gaggaagcgg
      181 gcctggtcaa gttcgacttt ctgggactca agacgctgac ggtgatcgac atcgcggttc
      241 ggatgatcga aaaggtcaag ccggactttg atctgcggcg gattccgctc gatgactccg
      301 gtgtctatcg gatgatttcc gatggcgata caaccggtgt gtttcagctt gaatcttcgg
      361 gtttcaaaga gctgctgaaa aagcttaaac ccgactgttt cgaagacatc atcgccgcgg
      421 gtgcactgta tcgtccgggt ccgctggaag gcggaatggt cgacgacttt atcaaccgca
      481 agcacggacg caccaagatc gtctaccacc accccgcgat ggagccgatt ctgaaagaga
      541 cctacggcgt catcgtgtac caagaacaag tcatgcagat ttcgcgcgcc ctggcgggtt
      601 actcgctcgg tggcgccgac ctccttcgcc gcgcgatggg caaaaagaaa aaggaggtca
      661 tggaaaagga aaaggccaag ttcctcgacg gtgcgaccaa gaccggagtc gataccaaga
      721 tcgccgatga agtgttcgat ctgatggcgg cgttcgcggg ttacggcttt aacaaatcgc
      781 actctgcggc gtatggactt gttacctacc agacggcatt cctaaagaaa cactaccccg
      841 cagagttttg ggcggcgctc cttacctgcg ataaagatga caccgacaaa gtcgcgcgct
      901 acgtcgcgga agtgcgcgat tccggaattc cggttctgcc gccgtgcgtc aaccaatcgg
      961 aaatggattt ttcggtcatc cgcctgagcg aaaagcatcc aaagtccggt gttccgatcg
     1021 aacgacaagc gattcgtttc ggtatgtcgg cgatccgtgg cgtcggtgaa ggcgcagtcg
     1081 aagtgatctt ggcagcgcgc aaggaaaaag ggaactttgc ttctctgttc aatctgtgtg
     1141 agcgggtcga tctgcgccgc accaacaaga aagtgatgga agcgctcatc aaagcgggcg
     1201 ccatggatgc gatcgttccg ggtcgcgcac aagcgatggc cgccatcgat tctgccgtcg
     1261 aaagagccca gaaagcacag cgagaaaagg aaagcggaca gacatctctg ttcggactcc
     1321 taagcggtgg cggagccaag tcaggaagcg actttggacc cccaccaccg tatccgcagg
     1381 tcgcagactg gattcccaaa cagaagctgg cgctggaaaa agaagcgctc ggattttaca
     1441 tcagcggtca tccggtcgat cgctacgcac cagagcttcg gcgggccaga gccgttgcgg
     1501 tcgccgagct ggccgacgat aactttgaaa ttccgcgtgg ccgcgatcgc gccgacatct
     1561 tggctggcgg tgtggtgtcc gacttccgtg aacgaatcct caagagcgga accggcaaga
     1621 tggcgtcatt ccgtttggaa gatccaaccg gcggaatcga agtcgtctgt ttttccaaag
     1681 cgtatgccga atatgaaacc gtgctaaagg ccgacgaacc catcttggtc atcggtcagc
     1741 taatgagcga cggtgacgga gaaaacccac agcgcaagat caacctgcgg gaagcccagc
     1801 ttctgtccca aatccggatg gaaaaaacgc agaagcttac gctttcggtg gtggcagatg
     1861 atctgacaga agccaaagtc acgtcgctga aggacgcgct gctggcacat ccggggccgg
     1921 tgcagatttc gattctgctg caaaaacccg gacgctggcg tgtcgaagcg aagcttccaa
     1981 gtgacatcaa aattcagccc agcgatgagc tactgacccg gcttgggacg ctctgcggcg
     2041 acgatgcggt tcagcttggc tgattccgat ccggtttagg acgcggtgcc aagctgctct
     2101 ttgacggcct ggcgcagcgc atccacatcg ggatagacgt gcttgggttt cgaaaagatc
     2161 gtgaaggtct tcatcaccgc gtctacccag ccaaacgtcc ggtacggata gccattctga
     2221 atacagaatt ccttcaccag cggtgccatt tttgcgtagt gagtgtggct gtacccagga
     2281 aacagatggt gttcgatctg gtactgcaga ccggaacata ggaatgcccc gatgggtcca
     2341 gtggaaaagt taattgtcgt cgctgtttgc agcaagacga agtcttttct ccagtcccct
     2401 ttggcgatgc acagcgcttc ttccggatag tgaccagggg ccagcacgca gtagagcgga
     2461 tagccaagca gaccgatccg cagcaggttg aaaccaatca ctgaaagaac cggaaagtac
     2521 agcgaaggaa tcacaaacca caccacgatg tgaagcgaca tcaccgccgc atcggcaacg
     2581 tgagcaagcc gtcgtttccg cttgtcggga agaatcgcga acagatgccg ccagctcccg
     2641 atctgtcgaa ggtagctgtt cagccacgcc atgcagggaa acacatagcg ctggctggcc
     2701 aggaaccaag ccgaaccctg cgcgacttct tcatcggtgc tcgcgaaaaa cggcccaaag
     2761 tctgcgtcgc catcaattcc catcacgttg ggattcgcat gatgcagcgt gatgtgacga
     2821 tgccgccaga acgtcgcgcc aagcatcagg aaaaacgggt agccaaagta cgtcagcact
     2881 tcgttgaccc agcgatgtgg acttgccgcg tagtgcgttg cggtgtgtgt attcgttccc
     2941 acaccgacgg aaccatacgt cgaaatcagc attcctattg cgcgcaacca ccacggctcg
     3001 caaaagacga agatcgcaat tccgcccaga gccaagcaca catgaacgac cagttcggcg
     3061 atcacttgtc cgacatgacg ctgattccag cctcggtttt tgaactctgt ctttagtgcg
     3121 gaaagggcat catccatcgg aacatcgtag cgcgcggaag acagctggtg gcatcaaaac
     3181 agattccgca aagagatttc gcagcctcgc agatacgctg tacttgccgc acaagaaatg
     3241 ataagcgcgc cgagttcagt tcaggatcgt cgagtcgatg ttccaggtcg tgcgcagcgg
     3301 tccatacccg acgccgaagc gtcccaccgg ctgaagttca aagaacaccg aaccttccgc
     3361 ttccaccgga acgctcagcg tggggttttt tccgtttagc agatcggtga caaagctgac
     3421 cgcgactttc tgaaacaccg acagcgcttg gtcgagtccc agcacaaaca ccagatcaag
     3481 attcacattc ccggctgctg cgatcgtcga cttgcgtagc tcttgctctg ctttgccggt
     3541 gacaagatcg gtgatgagct gcggaatccg cgcctgaaaa tcattgagcg tgcgaatgtc
     3601 cgtctggctc gacgtacagg caccgggctt cggctgtccg gtacacgtcg ggtcggcaga
     3661 accacacatg ctaagacaga tcgctccgag cgcttctgct tgttgtccgt caaacagccg
     3721 cagcgcaagc aacacgtcga gcgcaggcac tggcacgttg ttcggattgg taatcagcag
     3781 gtgaattcca aagttgaacg taagctggct ggccggtggc ggagaaccca gcgtcaccgt
     3841 acagagcagc cgcagcgtcg aatcggtgat cacagttgga cagtaatacg ccgcaagctg
     3901 ggtcacactg ggacgcttgg taagctgcag cgccgacgcc tgcaccgtcg gaggattttc
     3961 tgcattctgg gccagcgaat cgagcaaatc acagcttgcg acagacagca gcagtggcaa
     4021 aagccaaagc acagacagcg cggaaacacg acggaacacg gactttgcgc acgacgacat
     4081 gggcacctcc ttggcagata agacacagcc ccaccgcgat tgtgaggcgt ttcttcgcgt
     4141 gtgacgagac accagcgtcg ttccttctgc gccatcgccg tgttatagcc cccgcacaat
     4201 gtcgtccatg ttcgtaaaca gccccccacc cctcgatcgc gagcgtccgc atttgctggc
     4261 gattccgacg gggttttcgc ttgcgcaagc gattcggata agtccaattg gccttttgcc
     4321 gccgctgctt tatgacccgg tgcagccggc gatgcaggcg gtgattccca tcgcgcacgg
     4381 accttccgcg gcaccgcgcc cgctgcaaga agtgctgatt cgtggtgtgc ccggacacga
     4441 tgcgctgctc atctatggac tgggagaagg gccgcacgtc gaaccggtgc ttgatcgggt
     4501 ccgctggatg ctgggtctcg acgacgactt gtttctgttt taccacctgt gcagccgtga
     4561 tcgcgatctg gcctgggtcg ccgaacagca cgctgcccac gtcttgcgca gtccgacggt
     4621 gttcgaggat ttggtcaaga cgctcgtcgt cacccaaaaa gccaaacagg cgcatgcgat
     4681 ctgtcagacg ctgtgcgacg catttggcag tccgacgatg ctttcgaggc tggcgttccc
     4741 gacggaacag cagcttgccc tggccaccga agacatgctg gcaaaagagc tgggacttgg
     4801 atctctcggc acgatgattc atcgcttggc ccggcgctgc gccgacggtc agccgcatcc
     4861 cgaaacgctc cgccgacacc gtccgtcgat ttcgcaagcg ctggccgatg atgacgaaga
     4921 gctgttcgat gacttggttg tcgaggagct gcactggcaa gagcgcgtcg ctgagctgct
     4981 cttgacgttg ccgggctttg gcgtgcgtgc ggtgcccaaa atgctgcggc tgctcggctg
     5041 ctttgacctg atagagctgc accacagtgc gatgcagacg tttgcgaagc gctacccgaa
     5101 acgacgcaag actgcggaaa gcgaaaccgc ggaggaaatg accgatcggc tgctcaagcg
     5161 gctcgacggg tttgcgatct accgcagctt ggctcagtcg attttgctct tgccgccgga
     5221 acgcagcgca taaagatgcc gatcgtcggt gccgaccaag atcgcctcgt cttcggccaa
     5281 aagcggtgaa ccatcgacgt ctgcatcaaa cggcacttgc cacaaaagct gaccatccac
     5341 cgtcagcgca tacagccgat cgtcgcgcga accaaacagc accctgccct tgcggtcgat
     5401 gatcggtgcc gattggatcg catcgccggt ctgaaacttc cacaacaacg ttccgtcgct
     5461 gcgcaccgcg taaagctgac catcatacga tcctgccaag atcgtcccgt cgctgcgcag
     5521 tgccacagcc gcccgcaccg gagcgcccgt tgggacggaa aacttgatca cgccatcttt
     5581 cgacactgca taaatccgcc ggtcttcagc ccccagatac gctgttccgt cgggaccgat
     5641 cgccggcgct tccgccaaat caatcgcgag cgcgatttcc cagcgcttgc tgccgtcggg
     5701 acgcaggctg ataagcaggt cttggcacac cgcgtacagg ctgccgtcga agcccaagct
     5761 gggtgacgat ccgcagtgtt ttttgtcgac ggcgtaatgc cagcgcactt tcccgtctgt
     5821 cgaaagcgcc gtgatcccat cgccaccgac gtaaagcgat ccatctggtg caggcgtcac
     5881 accctcaatg tcacagcgcg acgcttccgg accgaccccc acgctgcgcg cacagcgacc
     5941 cggtgccgcc gtccagcgca cactgccgtc ggacagattc agcgaataca gcttgtcatc
     6001 gtccgatccc accagcacca aaccgtccgg cgtgaccacc ggcgcggaaa acaccatgtc
     6061 gagcgtcgcc cgcttccagc gcagtgtccc gtcgggatgt agcccgtaaa tcgaataatc
     6121 atgcgatcca aacaccaccg ttccgtcggg ggacaaaatc ggtgcggcgg tcactttgcc
     6181 tttggtcgca aacttccaca ccacctgcgg cctcttgtcg ggaagcacaa acgggctgcg
     6241 tcccgtccga tggagatcgc cgcgcggcag catccaggtt ccccccggca gcgaaagcgg
     6301 tggcggcggt gccggcaaaa gaccgcttcc tccatcggaa attggtccga tcccagcgtc
     6361 acttggcgaa ggattttcag cagcagagga agcgtcgacg gaacccgcat cggcagcgac
     6421 acggaccgcc aaaatggcgg gctcggcagc agaacggcgt tcgagctgac aggccaagct
     6481 aagcagcaag accgacgaca gcgcgagagc gccgtgcctt tgtgcacaca acttcacaga
     6541 agacagttta ctacggcagc ggcccgcgca cttctcccag gtcaacaaac gcgccatcca
     6601 ccatgatcac aaacgggcgc tcggtgaacc gcagaccgcc tttgccgtcg tgctcgatga
     6661 tttgcagctt gcccatgccg tagcccatcg gtccgcgcga gtagtagtgc gcctgaagtt
     6721 tgtagggata ggcccgctga cccgacggcg caaagatcga aaagcactct ggaccgtagc
     6781 ccgtcgtcac gtcggcatac agctcgccgc ccgatggcag cgacggattc gaaaagtacg
     6841 catgcccgcc gcgcccatcc aagatgtgga aatcgacatc attggcatcg gtttcccatg
     6901 acagcacaaa gcgcagcgac ggaccgctcg caagctgggc accgagccct tgcagccgcc
     6961 gttcgatctc ttcccgacgc tgcggttctt tgcgcagcca cgccgctgcg ataagtccca
     7021 cgtcttcggc caaaatgcgc ggtacgccgg caaaacgacc cgacggatag ctgcgcgccg
     7081 caccagagac gatcgcctcg aacgcttcgg cgggcttgcc cagcttgagc agcgcgtagg
     7141 ccagcatgcg atggctcgac ggatgatcgg gacgattcac cgccgctttg gcataggtat
     7201 ccgccgccag atccagtcct tcgctcagct tttccagccg cgcgcccgca aagcgacgca
     7261 gatcggcgcg accggggaac agatcgatga tcgaaccgta ggcacgggcc gccaagcgcc
     7321 gctgctgctg ggattcaaac acctcgccaa gaccaatcaa ggccagcaca tcccccggat
     7381 cgctgtcgcg ccacgcctgt gcccgctgca gcgcttcccc agcccgaccg gctttgagca
     7441 ggttcatcac gtcggcgagc ttgccttcat acgggttcac aaacggacgc tgcgacgcat
     7501 cgtcgtcttg attcaccgga cgcagcaccg agctgggcgg aaccggacgc atggccatcg
     7561 ctcgacgttc aactggctgg gcccgtggac gttcttcttc cttggctttt tcggcgcgat
     7621 caaagcgagg ctctggtcgc ggtggcggtg gtggcggagg gggagccgca gccggtgcgc
     7681 cgcccgaacc tccgatcatc ccgtccgcag cgtcatcctt tgaacgacgg ctttcacgca
     7741 tggcccgcgc gggtgccgcc gcgaccccct ccgccacgat cggcgcaggt gccccagttg
     7801 cagcgccccc cggtgctgac gccggcatcg acagcatggc accatctggc gccgagtctg
     7861 cagcaacggc acgtttgtcc gccaccgatg cagaagccgc ggattctgcc gccatgccgc
     7921 ggggtccacg ggttgcgctc ggtggtgccg gctttggcat gggccgtgac atccacgacg
     7981 gattctgcgg cggtggtggt ggcggcggcg gaatcgcctg agccaacgct ttgcgcgaac
     8041 gcacttcgat gccgtccatc ccgacgccca gaatgtcggt cagcgcccgc ctgtcaatgt
     8101 gaaagcgcgc atagtccgac tcggtttcca acaccagcag cgccgtaaaa tcacacatca
     8161 cgcgaaactt cgtcgacaga tcgatgatct ggtgcttcag cccctcgctg agatcgggat
     8221 cgcttgcggc catcgtatcg cgctgatgcg aaagccgcgc aatccgcgcc cccacccacg
     8281 cgcgctctag cagaggccgc ggcacttcca ccgtcgccac cccgatcgtt tcggtctggt
     8341 tttcgcgccc acgcaacacc acctgcgctg cttgttgatc gggcacatcg gcgtagacca
     8401 gcacctgatc gccaggctgc acaccgtcga gccgcagcgg ccacacccac ttgctgcccg
     8461 gcacgctgac ttcgatgccc gaacgcaccg cccggcgcag ccgcaatgcc agcgcttgtg
     8521 tcggccaatc gccgtcgagc acaaccccat ccgaagcgag ccccatcgtc gtcagcttgc
     8581 gcaacagcga ttcatcccgc aggccgccca ccgtcaccgc gtcaagccgc gcaatcccaa
     8641 ttcccttgag cgcctgcacc gatttttcaa gctgtggcgc ttccgtcgga cccgccgtga
     8701 ccacgccatc gctcatcaaa agaacgcgcc gaaaactgct cttgctttgc gcgaccacgc
     8761 gcagcgcttg tgccaaatcc gatgcaccta gcgcccgccg tgcccgcagt ttttccagca
     8821 cgcccggccc gacatcaccg acgctgccag aataaatttc ttccgtcgtt tggtcgaatg
     8881 ccaggatgcg cagcggtgtg cggccttcac cttgacggga aagttctgca cacagactgc
     8941 ccagcagatc gacctgtgct ttgtaaccca gcgcgcgcga tgcactggtg tcaaacagca
     9001 ccaccagtcc ttcgatcggc tcgatgccca ctttgcccag caccgtctgc ggggtgatcc
     9061 gcgcgaccag caggttttga tgcacaagac cgaccgcgcg accatcattc gccgttggca
     9121 caggcacccc gacttcaaaa tccacgtcgg gggtaaagtt gtctttttcg accgtcaccg
     9181 tctggtgtgc caggctcttg ccaccgagcg acgacgtcag cccgccatct tgatttttgg
     9241 cgacgatcgc gcgaatcgac agtttgctga gcttgggcaa gccgcgcaga tagatgcgaa
     9301 agggatcggc agcgttttgg cgttctgccg aataggacac gatcagctct ttggtcgccg
     9361 acggcggaat cggaaacacg cgcgcccgga agctgttgcc cgcctgtttt tccaacagcg
     9421 ccggatcttg gcgacggtgc aaaaagtctt cgtaagcccg acgtgcggcc tgaagctcga
     9481 cgacttcgcc ttcttgccag tcattgcctt ggcgcatcgc gaagcgcgaa atcgcggcac
     9541 cgggcggcag cacgatctcg aactgacctt cgcgcacccg tggctcggga ttgcggaacg
     9601 tcagatgcag ctcggtaaac gtcagcggat cttcgaccac cgctttggct tccagtgaaa
     9661 ccagctcaag cccggcgcca tcggacgaag tcagcgacat cggagcggct gcgggacggg
     9721 caagctgaag caccgggtcg actggcttca cttctcgcca gatgatgctg ggctgcggcc
     9781 tagggcgaaa cggaccgggg gcgacgtcgg caacggcaga attggaaggc aacgtcgcag
     9841 acagaaacgg aagacaggtg acagcaagtg ttgctagcca ccgagcaagg ccagtcgggc
     9901 gccgcatgta aacaactcct tggggcaggg tcgcgaccgg ggtcgcaatc gatccatatt
     9961 acgagccaac ggtgaaccga gggaaccttg actttgcttg tgagacaccg tgagcttgag
    10021 cgcatgaacg cacgcttttg cagtgggatc ttggtggccg cggcttgctg ggcgttgggc
    10081 tgtactccgc cgccgacgcc accgattcag tttcaggccg gcaacctcgc gcgggaaccg
    10141 atggacgcgc tgctcgaatc cgcaaaaaaa attggcatgt atcccgcgtc ggtgacgccg
    10201 ggtctcatcg aaacgcgatg ggaagaaacg gcgatttttg ccgagccgct gctggaaaag
    10261 aaaaccaagc tggtgcgacg ttatctgctg cggattgaaa agcgcgcgtt tggccatcaa
    10321 gtcaccattt cggtccaggc aaggcgctgc attccgtcca ccctccgttt tggcgaaacc
    10381 gatgtcgaag gccagtgtgc ggaagtgccg acgctgccac cgccgcttgt caccgaactt
    10441 aaccgaactg cggatcgaat cgaacagcag ctcgcgattc cgtaacctgt tgcgataagc
    10501 ccaaggacat ggacgatgga caccaatgaa cgacccgctc ccgtcgatgt acggattggc
    10561 ggaacggcgg cgcggctgct tctgtcgctg atggaacagc tccagaccga agatggcggc
    10621 ggcgtgatca cccgcgcact ggggctgctt gaccttgcct tggccgcccg tcgacaaggc
    10681 aagtcattct gtctggttga tccaaagacc ggcgacagcc aagagatcgc tttctaactt
    10741 ccgcgtgttc gattcccccc gctttcgccc gtttgttctg tggctacagt ttcgccgccg
    10801 cttgtcgtat atctgccggc atgcagcttg accctgcgat ccaaaatcct cccgctgaaa
    10861 ttgcgccggc tcagacgacg gaaaacgcag gcagcgctgc atccgatggt tcattgccca
    10921 aaaagacgcg cgggcaactc ctcttatcgg tgggtctgct ggcgggcgga acgacgtggg
    10981 ctggagatgc gctcggcacc ggcagtctgc tgttgggact tcggctgtat cgctcgatcg
    11041 cgctcgtggt ccaaggtcgg ctagggtacg gacggatcga tcaaagacag ctcaccgcca
    11101 ttttgatagg tcttatgggg ggcggatatt tggcaaacaa agtctacggt cggctaagcg
    11161 tttcgtttgt ccaccagcat gaggaatcga tcgccgccgt cgctgaacaa cccggtgggg
    11221 cgcttttggg aatcggaacc ggaatccgcc atcgggccgg cgtccaaggc aacttgggag
    11281 tcgatttcat cgtctaccgg cagcccaagt atgagctgtc gctcggtccg gaagtggcca
    11341 tcagctatct gacctattcc actggaccca gcatctatgg gtttgccggc ctgtctgcag
    11401 gcagcgcttt ccatctgttt taggagcccc aagatgctgc accccttttt cagattttcc
    11461 gtcgcagggc ttgtcctttt ggcgacttca tcatgcggtc tgttttacag cgacatcgat
    11521 tcctatccgt gtccccctgc gggcacccag ctgcgctacc agaactttgg cgccgccttt
    11581 gtggattcct actgtcaaag ctgtcacagc tcccaatcga tcgatcgcca gggcgcccct
    11641 ggtgagttca tcttcgatac agtcgagcag atccagcgtc acagagcgcg aatctttgtt
    11701 cgatctgcgg cagacaatga ctcgatgccg cccggaccca atgacccgtc caaagagcaa
    11761 cggaaacagc tcgccgagtg gctggcttgc ggcgccccca actaaccgac ttccgaaata
    11821 agagtcattt tacatgcctc ctatgaaacc cccgatcaag ctgcccaccc tgccctatcg
    11881 aaaacccgtc gaaggacgcg atctgtggat cctcgacaac gcacttccca acgtcgatga
    11941 agtgcgcgcc cgctgcttgg cgaaaaccga ctggacgtat gggtttcctt atcgggatga
    12001 aggatggccg ggaatgcgtt cgattccggc gctgcttccc gacgagcttg cgccgatcga
    12061 agactgggtg cgcaaggtca ccggatacaa aagactgtgg caaaatccga cgccggaagg
    12121 aatgacgcta aatcacaact gcgtgcaagt ggtgggagtc gatgaagccg atgcgcgacc
    12181 gcacacggat tcccgaccga actgtcgtct cgcggcggtg atttatctta cgccgggtct
    12241 atcgcccgat tgtggaacgt cgttttaccg gcagcgactc ccaggtggaa aactgggagg
    12301 gaatcacgtt ccgccaccgc acgcgaatct gcaagcagcg ctgggaacgc gctttgtgcc
    12361 gcccaatagc ttcatcgaag atgcccgcgt cgattacaag tacaaccgct tggtgatgta
    12421 tcacgccgac ttgattcaca gcgcgacgcg gtatgttggc aaatcgctcg aaaccaagcg
    12481 gatggcggcg gtgtttttct ggctggcgca gtaatggaac gtcagacacg cgcgactatc
    12541 cacacgccgg cgtcgataca attggacgca gtttgtgaca cgcagcgtgc agaaactcta
    12601 catcggaaat cgagtaaaga cgtgcttcgc cgtgcgccaa aagcacaacg agctgtttcc
    12661 catccgatga aagctctgcg gacagcggcg ggtacggcaa gtctgaaagt gcaaacacca
    12721 agctgccatc ttggcgtgaa aagatgcgca ccgaacggtc tgcgctggat gtcacgatgt
    12781 agtgtccatc aagagaaaac tcggcagatc gaatcgatcc ggtgtgtccg cgcaggtttt
    12841 gcataagctc tccggtggtc gttccaaaca ccgccacgga accatcgtct gcaccggaca
    12901 cagcgacgga atctccggcg gcatgggaca gtgcaatcgt ccgcacccgc gcagcatgtt
    12961 cgtgcagatt ccgcagccgc tctccgctta cggcttgcca caggagcaca cgctttcccg
    13021 ctgccgtcat cacaagctga ccattttggg aaagctcgat tgcagtcact tcatcgacgt
    13081 gcaaaagctg cgaaccgact ttgtgtccgc cttgtcgcat gtaaacgact tccgcgcgtg
    13141 ctcccattcc gatcaccagc ggaaaaccct gcggcggatc atcatcggga gacaccagcg
    13201 cggacaagac ctgcgccggc tgcggattgc gcagttgatt gagcagctgt ccctgttccg
    13261 taaatgtgag cacgcgattg tcgacggaaa tcatcacctg gctacagcga tatttggccg
    13321 ccaaggaatg caccacggct ttgtccggaa gtggaaattc cgcagacagc ggctgccctt
    13381 gtccatccca caacgaaacc ccgctctgcg atgcggccag cagaaacggc ttcccttccg
    13441 cgctgcgagc aaacggacac agcatcaggt gatgcagtcc gggttccgaa acgcgcagaa
    13501 atgggagccg cggcgtcccc atgtgcccga cggcctggcg cagcttcagg gattccggct
    13561 gcgacaaatg gcctattttc ccagccagtt ccgtgaccac atcgcgaagc tgtagcgctt
    13621 cctttgccag cggtggaccc acacgcagcg atggacttgg ttcgagcgcg cgcagtcgtt
    13681 catcaatgcg aagccgaact ttttcgtgtt cctggctttc gcgctccgcg gcattctttt
    13741 cttgctcacg gtgccgcaga cgtcgctcga cttcgctctt ttctcgtcga agatcacgca
    13801 gacgcagctc ggtttcatgt ttctgtaagc gcagcttgcc aagctctttt tctttgcgca
    13861 gaagctgctg acttgcatca cgcagcgcac tgtaaagttc cccgacttct ccttcgagcg
    13921 attcaatccg tgcggtcagt ttggcaatct gctgctctgc gtaggaacga tattcctgga
    13981 gcagcttgat ctgggaatcg cgcaccgcag ctttttgatc ggcatctttt ttgtccagaa
    14041 cccgccgata ggtctgatac gccgcgaacg gaagcagtga aaggagtccc accaccgcga
    14101 gtgcaaaaat cgtccgctgc cgtcgctctt cacgatctcg cacttgctgg ctgcgaccga
    14161 taaactctgc cagatcagga ggcagggact gaccgctttt gggaatcgta tccagcgctc
    14221 tctcgatctc gatccgcgac agagagtagt ttgcgcctcg tccgtgttcc cagtacgcaa
    14281 acagcttgtc ggtcagccag tccggcgtgc tgcggtcgcg ttcctgaatc cacttgctgc
    14341 cgaacacctc gcggaaaatg cgattgcgcg caatcagtcg ccgttccgtt ccatcctgcc
    14401 gcagcttgac cattcccgac agaagcagca gcgaatgcag ccgatcctgc ggttcataga
    14461 tcacccgatc tccgacaaaa attcgccgat acagtgtcac tgcactgtgg cgatcgtgat
    14521 gatcgctgac atgctggaac tgcgcttccg caaagctcaa gttgggatct ttgtgtcccg
    14581 ataggaacaa tttggcgacg gcatccttga cagaatcagc aggattccca ttgtctgtct
    14641 gcggcttgtc aaccaagtac tgacacaccc gctgcgtcat gtacggatga ccgtctgtcc
    14701 atgacaacac atgagccagc cagtccgcac tgttccccgg cagaaccgac agaccgggca
    14761 gaagcacttc ggcttcccgc gccgtgaagt cgtccaaata cagccgcgtg ttgtcgaggt
    14821 taaaaggcga acggaccggg tctttgatca gctcttccgg tgtggcaacg ccaatcaaac
    14881 aaaatgtaag ccgcggcttt ttcgaaaagg aaccacggac ttcttcatac agagagcgca
    14941 gcgaagcaaa aaatgcatcg gtggaaaaac ggaaatccgg actcagaacc gtgtcgatct
    15001 cgtcgaagaa gatcaccacc ggcgcatcaa tgtgcggcag cacttcttca tgaaaaaaac
    15061 tggtccacag atgcaccgga cccgattggg aatggcgcga aaaaaagtcc gacgcggaaa
    15121 atggcagctc cagttctttc gcagtgcgct gaatcagacc gcgataccac tccgcttccg
    15181 ggggaaaacc aaagccttgc agattcaggc tcgctgcctt cgcaccacga cgacgaagct
    15241 cgctcatcgc gcgcacccgc aggcttgatt tgccaatctg ccgcgttccc aagatgtaac
    15301 agagccgtcc gctgaggagc tcggaaacca agcggtcatc ttcgggacgc tcgacgtaaa
    15361 atgcctgtcc aagcggaacc acgccccccg catgaaagcg tcgggtcggg aacggactca
    15421 ttttcgcgag gttttccgac gacggcaaat cgatgattcc tttttcctaa agcccgcgca
    15481 gatagtcttc gtacagcgga taacgcagct tgatgccggt ctgggtttgg cgcgtaagtc
    15541 cacttgccat aagtcgctgc cgttccgcca tcgtcagggg tccgccgcgc aaaagtcgct
    15601 gcacagaatc atccagctct ggactgcgcc gaatccactg ccgaatcgac tccagatgat
    15661 gctgacagac gcgaatcagg tgttctcgat ccagatgcag cacaccgagc cgcgccgcat
    15721 catccaaaat ccgccgcaac aggtttggct gtccaccgac gatttgcaaa agtgcatcgg
    15781 tcgccacatc cgcccccggc aatcgatcgt agccgcggat cagcgccacg acttcttccc
    15841 gcgtgaagtc atcgacttcg atgcgctcgg cgtgattcaa aaactgtgat ccgtgaatca
    15901 catcctgcgc cgattccagc gtcgaaatca cggtcacaag tcgcagagac aaccagcccg
    15961 cagaatccgc tgcaccatcg cccgacagat gcagcgcctg ctgggtgcaa tcggcccagt
    16021 gacgcaacat tccgaacacc tcggccccga gcggactgtg caacagcaga tttcccgaaa
    16081 gcagaatcag cgacagccct gacgaaagcc gcggaaaaat gtcttcttcc aaaagccagt
    16141 tgagccgctc aatgtgatcg tgctgttcaa agcgcgtctt cacccactgc gcatagtctt
    16201 tttcgccgct gcgctgcgcc accgcccgcg ccagccgcag accgattccg cgcagcacgg
    16261 aatccaaatg atcgcggtga tcgagcttga agttcgccag atcgagcagc acctccagcg
    16321 gcttgcgacc ccgcttcgcc tcgtcgctgc gcacactggc cagcacatgc cgcagcagcg
    16381 tttttttgcc cgcaaacgca ggcccccaca gcaccaccgg attgtgcgtt tctccccgca
    16441 gataacggcg cagcggacgt tccagtcgtt cccgcggaac gtagtgttct agcgatatcg
    16501 ccgtaagccc cgcgtccgat
//
LOCUS       DAORGN010000105        16376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1449_length_16376_cov_5.677409, whole genome shotgun sequence.
ACCESSION   DAORGN010000105 DAORGN010000000
VERSION     DAORGN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16376
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_16376_cov_5.677409"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1144
                     /locus_tag="PKL17_12515"
     CDS             296..1144
                     /locus_tag="PKL17_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018809491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK45604.1"
                     /translation="MTNSSSTQTDLPLKLSGWAVILGASSGFGEAISLELARRGMDIV
                     GVHLDRRSTMPNVERIVGQIQALGQKALFFNQNAADEQKRKEMCDQMAAQMTSQTAGA
                     GSPRVRVLVHSLAFGTLRPFLPTGDGPTDVIGKSHLDMTCDVMAHSLLYWTQDLLSRG
                     LLGQGSRIFAMTSSGSHRVIAQYGAVSAAKSALESHCRQLAFELAPRGIAVNALCAGV
                     TDTPALRKIPGNEKMIAVATERNPAKRLTTPQDVAGAVAVLAHPLAGWISGNVIHVDG
                     GEDIAG"
     gene            1119..2063
                     /locus_tag="PKL17_12520"
     CDS             1119..2063
                     /locus_tag="PKL17_12520"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNK45605.1"
                     /translation="MAAKTSPVSHWPKPRALRPGDPVAVVAPSGPAPAARLSAGTALL
                     ADRYRVEQSDGLTQKLGFLAGDDGRRLAELRWALSDPRLAAVFCARGGHGALRLLPSL
                     LDVRGEELPARPLIGFSDVTVLHAFLALHKRVSIHGPVVTQLGELPASDRAQLFALLE
                     NSDPPPPISGLVSIGVSQRETTGRLLGGNLEVLSRLCGTPLQRVFSQSGPLVLMIEEV
                     TESPYRIDRALTHLRQSGMLDHVVAAVVGDLMRCEGPDGHPSALDVIVERLGGLGVPV
                     LAGVPLGHGARNRAVPLGVAVHVDPRRGQIDFLESAVR"
     gene            2155..2742
                     /locus_tag="PKL17_12525"
     CDS             2155..2742
                     /locus_tag="PKL17_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45606.1"
                     /translation="MSGTGLGPRRSIRGWSGFWGIVLFGILGVLSASGCSRTIGDACK
                     NNVECSALGDRYCDVASPGGYCTVEGCDQTSCPDSSVCIRFFSLQKGRQSCDASKVVR
                     TDCSGGDCCKPGDPGCCKLGERCLCEEPGCQSGYCTSEASERRYCMKPCDSDSDCRTG
                     FQCTVTGSSGALSVVYRDTDGNIQQPEQHYCAPPK"
     gene            2755..3789
                     /locus_tag="PKL17_12530"
     CDS             2755..3789
                     /locus_tag="PKL17_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45607.1"
                     /translation="MTGEPLGQKSKLRRLCATGWSWTTRVFEALLSTSWRLRRYLVRR
                     LFSSLISVVGVVVLTFVLLHLIPGDPVDNLLGEDARPQDKIELRRCMDLDQTMPVQFG
                     RFVKSIFDGTLGYSCPDRKLTVAQRIKKALPPTITLAVSAMAVALCLALPLGILAALK
                     AKTRWDGALTAVALLGISMPSMWLGPMLLYLFYVLLPWFPGPGEEAGSLRGLVLPSVM
                     LGTHLMAMLARMTRSSLLDTLSEDYVRTARAKGLPAWKVVLKHAMRNALLPVITVAGM
                     QFGALLAGAVVTEKVFARPGLGTLLLEAIAQRDYRVVQGLTLVIAVMYVLVNLIVDVL
                     YAAVDPRIRV"
     gene            3786..4634
                     /locus_tag="PKL17_12535"
     CDS             3786..4634
                     /locus_tag="PKL17_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45608.1"
                     /translation="MSGSADKRWTWPPLGFAAWFGAALLLLFVLIGLVGPLLLKTSPS
                     AQDLDHLLEGPSRRHWLGTDENGCDLLTQMCYGARLLLGLCSSVVSLCLSLGLCIGVS
                     AGYLGGWFDELVMRVIDVLLAFPGILLNIAIVALVPKAGVGVLIFALAVNGWVGYARV
                     ARGQVLRVREQEYVLAARSVGCSPWRVMARHILPNIWSPIIVQATFGLAGVVLVEASL
                     SFLGLGPPVPYTWGALLAQGTNFFWRTRHLVLAPGLSIALLVLGCNLLGDGLRDYVDP
                     KRKRVR"
     gene            complement(4631..5128)
                     /locus_tag="PKL17_12540"
     CDS             complement(4631..5128)
                     /locus_tag="PKL17_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45609.1"
                     /translation="MSAHFPAPRKAPRPIASHLVAAALSGLSAWALALSLPLSAHAAE
                     PTAAKAPAATGKPVAKKSKGKAKGGGTAAELTQMLITKGPAVQDCAVNHGLDKGANSV
                     AIVTKVTINGRGQVINIQTSVNVDKGEGDKVRDCVNELIKSIRFPESAAPMITIERNW
                     TIKST"
     gene            5269..5655
                     /locus_tag="PKL17_12545"
                     /pseudo
     CDS             5269..5655
                     /locus_tag="PKL17_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019676495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5728..6192
                     /locus_tag="PKL17_12550"
     CDS             5728..6192
                     /locus_tag="PKL17_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNK45610.1"
                     /translation="MTPKNIVVATDLTELSEPALRMAKSLAEQLGSTITLVHVLEPAP
                     TPPGLEAFALEGMPLDWEERLLQGRSEAASRRLADTAAAETSAKVKVHWRLLQGRMPD
                     ALIEELKAIGGDLLIVGTHGRKGVSHLLLGSVAEKLMRGATCPVLVVRPTAS"
     gene            6205..>6454
                     /locus_tag="PKL17_12555"
     CDS             6205..>6454
                     /locus_tag="PKL17_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45611.1"
                     /translation="MARAAFFDMDLTLLRVNSGSRWVKYLRKRGEIGLPMLIRSGIWL
                     LQYRLSLLDMESVAAMLAKDMTGDSEAETRTKVLEFYRT"
     assembly_gap    6455..6464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6465..6901
                     /locus_tag="PKL17_12560"
     CDS             <6465..6901
                     /locus_tag="PKL17_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNK45612.1"
                     /translation="TRHKVAEFYRTELQHTISPDARAQLDQHRQDGDAIVLLTSATPY
                     VAEPLAAELSIPHVLCTRLAIENGRFSGRVMPPACYGSGKVHHAEQFAKAHGIDLAQS
                     SFYTDSYSDLPMLLRVGRPVAVNPDRRLLRYARRQGWPTLRW"
     gene            6929..7813
                     /gene="nadC"
                     /locus_tag="PKL17_12565"
     CDS             6929..7813
                     /gene="nadC"
                     /locus_tag="PKL17_12565"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNK45613.1"
                     /translation="MTPTLKVPDAQVLLPPAVARLIDLALDEDLGRGDVTSEAIFGSH
                     ETAQAVILAKSELVLSGLDVAMAVFQAVDRRILTTALFADGSSIHPQTQVLRVEGPLR
                     SILAAERTALNFLQRLSGIATLSRRYVQAVAGTSARIVDTRKTLPGFRFLDKRAVRHG
                     GAHNHRADLGSGVLIKDNHVAAAGDVAEAIRRAREYAPHSVRIEAEVTSIEQVSKALA
                     ARADIILLDNMSPEMVREAMATLPNVGDPRRPLIEVSGGVSLATVRAYAEAGADLISV
                     GALTHSVTAADLSLEVEV"
     gene            7830..13244
                     /locus_tag="PKL17_12570"
     CDS             7830..13244
                     /locus_tag="PKL17_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45614.1"
                     /translation="MSSEPHSLTHQSPFGEESLGPGRLRLKLTMPVPLDGGRLCDLSV
                     DIEGRPGDSLLHRRGRVKSAVVEVSQAQLSRVIATQLPSLCAQLPVGLVAPDRLAVRF
                     CGSDDKPQLFVVARFPDAGPQPLWCSLRLPLGPGRQDLRGPRRSSEPLTLSLGLYDAD
                     LRVYGLAQRSVALLGLSAAICVGAALLRFRSASTESVVLPATEFAPGLGMRLLFAHGL
                     REVGVDVLWDSLLQVMPRHGQRVADAKSAALALIERQDGLLRLSYEGLGSSFDFDDDP
                     RGRSTVDGGQIESLLLADRLLIEGDVPAALAAYELVGRVASFSDAAALRRAQILATVP
                     KRSDEVLPILDGLAAKRSFDIGTQLLALAVRVAGSRSSSSALVAQRDALCALAQSEEH
                     TAEEQALLMLWAAQLPVGDTDSLGLAQKALERVSADPNCPTAALVADGAAALLQALST
                     RLLFESSSLPPAVESSEISTELALADRCFVAGELSTAAVHYRRALVSDQVPKAELARV
                     HMRLAEIAHHHSDLAEEEAELGLAVECGGGAAAWSALAALFQSLGDGARLGVALYAWS
                     LHEVGDARADLLRQASRHVGPSLLLAIDEALLQIGADDELLRERLLRRRKQQDDHVGL
                     LSLLIRDMHQSSGARRWASARLAADVARRLGDVVTEAEATLLALSAPANPSVTDDDAM
                     RAIGLLAGEQRTLIPPETVSTLRNRLKARGSLRTVLRRLDQRLALLASATAPTASLVE
                     LYRQSALCMSLLDEWAAAVVRYLRAAALGDRTVLPEVRKLLALLLADGQHQQARRLIE
                     AELRRCPTEQAAPLRVALGEVLLRSGRPREALGQLELSLLRDEELPVAHALLGQILHG
                     LGQPEDRPRALSHLLEASQSSSLSPVETGECALLAAQMLLASEATVRLDADLAVANAP
                     TLPKEVQKQAERMLQRAIEFLPKDPRPRRLLISHHVAKQQPERALSLCDELLELAQTD
                     AERADVLWQKSQLVRDESEAEALLQSALLHDENHLPTLTRLRARAEQRGDKATALSWL
                     SRELVASPSDSERALLLCKQAELLDPQSQADLILTTLRKAMQLGLGQAGQRLAALLWA
                     RGDLLQAADIAGRAAQLLPPSEQGNQLLLAAEWALRVGDELRAREYLRQASEQPGESA
                     EEAAQRLVVLDGGDEPAHRRRTLENRLTQVSTGLSSIETLRQLLILCARQNDLVSVER
                     YAQALLSQVPLDGLGLTALTDCLIATGRSQESVFPQLQISREYPRRGPVLEARAEWLI
                     KRGESSEAESHLTEALAHAARGPERLRIGQQLADLHRRRNAHGLAAATLSGLLSDVDE
                     REARAALRRRIAALYVQAGQPLVAIEELRSLLAELPTDLLALEQLFGVALGCDDLQSA
                     RACLDQLVAHSTGTIRARWLCRRAELHQRQGSLSDALSDAEAALASSQDPDLLRALLL
                     LGVQLSDGGLIRQTTMALHAQGAPFLAASALAGCGLLLHAIGPPEQAERLLSPLETPD
                     ELALAMAQSVAGFRGPLHDVDRLLDPLRRRKLPRFETLRKLLEQRAFGQTVDLGALKL
                     LARLTEADWAPLHALYLSALAFVDPLGEPAQRLDVLPKVRFSPSEERLPEPTDELRPL
                     ATLLSHFARLLLRDTSSRGGVDLGPVQRVRERQAQLFAKMGGELVGDLSSLRALLGAA
                     TALWLPGHEPKTEAERQWLRSLQARALLPGQPSLTTEQAHLLLDPVRHELATSPEKVT
                     QQLGQTRELFHRRALLLAAVEQHDLRPALWSLLPAHEPMGEQSHGPQPGEPTEPHDRL
                     ALLSQPPLRTLLLDAQRLLS"
     gene            13296..14477
                     /locus_tag="PKL17_12575"
     CDS             13296..14477
                     /locus_tag="PKL17_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45615.1"
                     /translation="MAASANMQALVGTTQGNTYTLERLLSEGRHGALFEAKQIRLGYR
                     CFVRLLTVDPAQRKPLLTALSQQTQIPHPGLCPIVDTMQLSDETLLIASPLLAGQDLG
                     QRVAGQGKLSLSEGMAVLRTAAGALHALHQKGLCHGGISGQSLLIVGFDDVAVDGALG
                     GGQVNQRVVLLDSGLYLAQKERASAADDQAALAKLILDTVSDLSPQLQKVLGTAQDAS
                     QGRRYPSIQAMWQAAEAALGRKVVAAQPTAVVGALKLPKMDERGSRWPLLLASASFLM
                     LAAVIGIVGLRRPAPPPDSAKLLNRGAVETDQVTISLELSPETAKVWVAGKLLQSPLK
                     LPRSSTEVPLRIEADGYLPALSKVIPDRDRSLHITLAKAAEAAQALEAVKKPHRSRSK
                     K"
     gene            complement(14493..15434)
                     /locus_tag="PKL17_12580"
     CDS             complement(14493..15434)
                     /locus_tag="PKL17_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK45616.1"
                     /translation="MVRNWTIGALLGLGLLSGCVTAGTHQAKLDELARAQADLAKSRQ
                     DNVDLNKERDELKKRIQDALAQVGDLSGRLNQAESKAKGLATERDQYARLLNDSTALV
                     GQLKERLEKLGQNVDKLTTERGQLQAGLTDAKTRLEELRKQKAAAEARLQMFRSLVDK
                     LRAMIDAGQLKVVTREGRMLIALPDDVLFDSGKAEVKPQGQEALGKIAQVLATIPDRR
                     FVVAGHTDNVPIRGGRYRSNWDLSAARAIEVTLFLVSKGMKPQVLAAAGFGEFDPVSA
                     NDTPEHRSQNRRIEIVLQPNISDLPSLDGLDGAQPAK"
     gene            15624..16100
                     /locus_tag="PKL17_12585"
     CDS             15624..16100
                     /locus_tag="PKL17_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45617.1"
                     /translation="MLWQTDVAAVPSDLAQTSSDLSTPMCGWQELSSMCKEHSAWVSA
                     AARACCDLFGQQLRSLQFGSVCPQMGSFGARQVRFECCPSDDPNPTTHCETRKLGFGE
                     CLDDAIWKQRAATECGNSGRIVSSTSQRLSSPCGPGRYSGIVFDCCTIPCPTPITR"
     gene            complement(16090..>16376)
                     /locus_tag="PKL17_12590"
     CDS             complement(16090..>16376)
                     /locus_tag="PKL17_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45618.1"
                     /translation="PFVMRRYLDTAIQLCKLRSVEPSFLLHPLDFISGKECDALRFFP
                     AMNMPSSQKRDLLVEVLEKLAQHFRVVPMSEHALALRSRNLPRQKPNLSA"
BASE COUNT         2686 a         4686 c         5619 g         3375 t
ORIGIN      
        1 aacctgtgac cctcggcttg taaggccgat gctctaccgc tgagctatac gccctcaggt
       61 cgacccggtg aagcgcccag gtgggtgtaa ctcctaacag cctatggggt tttgggtcaa
      121 gcggaaattt tcaaaaattt ttcggtgatg cagcttggcg acgggaaagg ctccgcggaa
      181 aattgtttgg gtccgcagat ggccgcggtc cgaccggctt tccgggggcg cctcgcgggt
      241 tcggttggga cctgtgggct tttgccgggc cttgcggcgt ggtatctcaa acgtcatgac
      301 aaattcgagt tccacacaga cggatctgcc gctcaagctg tcgggctggg cggtgatttt
      361 gggtgcgagc agcggctttg gcgaggcgat atcgctggag cttgcgcggc gcgggatgga
      421 cattgtgggc gtacatctgg accgccgcag cacgatgccc aacgtcgaac ggattgttgg
      481 gcaaatccaa gcgctcggac aaaaggcgct gtttttcaac caaaatgccg ccgacgagca
      541 gaagcgcaaa gagatgtgcg atcagatggc ggcccagatg acttcgcaaa cggcgggggc
      601 gggcagtccg cgggtgcgcg tgctggttca ttcgctggcg tttggcacgc tgcgaccgtt
      661 tttaccgacg ggagatgggc ctacggatgt gatcggcaag tcgcatctag atatgacctg
      721 cgacgtgatg gcgcactcgc ttttgtattg gacgcaggat cttttgtcgc gcgggctgct
      781 cgggcagggg tcgcggattt tcgcgatgac gtcgtcggga agccatcgcg tgattgcgca
      841 atacggtgca gtgtcggcgg ccaagtctgc gctggaatcg cactgccgac agctcgcgtt
      901 tgagcttgcg ccacgcggga ttgccgtgaa cgcgctgtgt gctggtgtga cggatacccc
      961 agcgctgcgc aagattcccg gcaatgaaaa aatgatcgcg gtggcgacgg aacggaatcc
     1021 ggccaaacgc ctgacgacgc cgcaagatgt cgccggggcc gtcgcggtgc ttgcgcatcc
     1081 gctcgctggt tggatttctg ggaatgtgat tcacgtcgat ggcggcgaag acatcgccgg
     1141 ttagtcactg gccaaagccg cgagcgctgc gtccgggcga cccggtggcg gtggttgcgc
     1201 cgtcggggcc tgcgcctgct gcgcggcttt cggcggggac ggcgctgctt gccgatcgtt
     1261 accgcgtcga gcagtctgac ggacttacgc agaagctcgg gtttttggct ggggacgatg
     1321 ggcgacgact ggcagagctg cgctgggcgc tgtcggaccc gcgacttgcc gcggtgtttt
     1381 gcgcgcgcgg tgggcacggc gcgctgcggc ttcttccgtc gctgctcgat gtgcgtggcg
     1441 aggaactgcc ggcgcggcca ctcatcggtt tttcagacgt gacggtgctg catgcgtttt
     1501 tggcgctgca caagcgggtg tcgattcacg gacccgtcgt cacccagctt ggcgagcttc
     1561 ccgcgtcgga tcgggcccag cttttcgcgc tgctggaaaa ttcagatccg ccgccgccga
     1621 tttcgggtct tgtgtcgatc ggggtcagtc agcgtgagac caccggacgg cttttgggcg
     1681 gcaatctgga agtgctgtcg cggctgtgtg gaacgccgct ccagcgggtg ttttcccaga
     1741 gcggtccgct ggtgctgatg atcgaagaag tcaccgagtc gccgtaccga atcgaccgag
     1801 cgctgaccca cctgcgtcag tcggggatgc tggatcacgt cgtcgcggca gtggtcggcg
     1861 atctcatgcg ctgcgagggc ccagacggac atccgtcggc gctcgatgtc attgtcgaaa
     1921 ggctcggggg cttgggcgtg ccggtgctgg cgggggtgcc gctcggtcac ggtgcgcgca
     1981 atcgcgcggt tccgcttggc gtcgcagtcc acgttgatcc gcgtcgcgga cagatcgact
     2041 ttctcgaatc cgcggtgcgc tagtccggca gatgccatcg tcgccgcacc tgtcgtcgca
     2101 tacagaaaga agaatttgcc ggcccgcgcc aagcggtgct aaggtccgcc gcctatgagc
     2161 ggcactggct taggcccgcg acggagcatt cgcggttggt ccggtttctg gggcatcgtc
     2221 ctctttggca tcctcggcgt tttgagcgcc agcggttgct ctcgcaccat cggcgacgct
     2281 tgcaaaaaca acgtcgaatg cagcgcgctc ggcgatcgct actgtgacgt ggcgtcgccc
     2341 ggtggctact gcaccgtcga gggctgcgac cagacatcgt gccccgattc atcggtgtgc
     2401 attcgctttt tttcgctgca aaaaggccgt cagagctgcg atgcaagcaa agtggtgcgg
     2461 accgattgca gcggcggcga ctgctgtaag cccggcgatc cgggttgttg caagctcggc
     2521 gagcgctgtc tttgcgaaga gcccggctgc caaagcggct attgcacaag cgaagcgagt
     2581 gagcgtcggt actgcatgaa gccgtgcgac agcgatagcg actgccggac cggtttccag
     2641 tgtaccgtca ctgggtcgtc tggcgcactg tcggttgtct accgggacac cgacggaaac
     2701 attcagcaac cagaacagca ctactgcgcg ccgccaaagt agcgggtggt gacgatgacg
     2761 ggcgaaccgc tcgggcaaaa gtccaagctg cggcgtttgt gtgcgacggg atggtcgtgg
     2821 acaacgcggg tttttgaagc gcttttgtcg acgagctggc ggctgcggcg ctacttggtg
     2881 cggcggctgt tttcgagcct gatttctgtc gtcggtgtgg tcgttttgac gttcgtgctt
     2941 ttgcacctga ttcccggcga tcccgtcgac aacttgctgg gggaagatgc gcgtccgcag
     3001 gacaaaatcg aactgcgccg atgcatggac ttggatcaga cgatgccggt ccagtttggc
     3061 cgcttcgtga agagcatttt cgacgggacg ctcggctata gctgtcccga tcgcaagctg
     3121 acggtggcgc agcggatcaa aaaagcactg ccaccgacga ttacgctggc ggtttcggcg
     3181 atggcggtgg cgctgtgtct ggcgctgccg ctcgggatct tggcggctct caaagcgaag
     3241 acgcgctggg acggggcgct gacggcggtg gcactgcttg gcatcagcat gccgtcgatg
     3301 tggcttgggc cgatgctgct gtatctgttt tacgtgcttt tgccgtggtt tccggggccg
     3361 ggcgaagaag caggcagcct gcgaggtctg gttttgccat cggtgatgct cggaacgcac
     3421 ctgatggcga tgctggcgcg gatgacgcgc tcgtcgctgc tcgatacgct ttcagaagat
     3481 tatgtgcgga cggcgcgggc caaaggtctg ccagcgtgga aagtggtgct aaagcacgca
     3541 atgcgcaatg cgctgctgcc ggtgatcacc gtcgcgggga tgcagtttgg cgcgctgctc
     3601 gcgggggcgg tggtgacgga aaaagtgttc gcgcgacccg gacttgggac gctgctgctg
     3661 gaggcgattg cccagcgcga ctatcgggtg gtgcagggtc tgacgctggt gattgcggtg
     3721 atgtatgtgc tcgtgaacct gatcgtcgac gtgctgtacg ccgctgtcga cccgaggatt
     3781 cgcgtatgag cggctcggcg gataagcgct ggacgtggcc gccgctcggt tttgcggctt
     3841 ggtttggggc agcccttttg cttttgtttg tgctgatcgg gcttgtggga ccgcttttgc
     3901 taaagacttc tccgtcggcg caggatctcg atcacttgct ggaaggtcca agtcgtcgtc
     3961 actggctggg caccgacgaa aacggctgcg atctgctgac acaaatgtgt tacggagcgc
     4021 ggctgctttt ggggctttgt tcgtcggtgg tctcgctgtg tttgagcctg ggactttgca
     4081 tcggggtgtc ggcgggctac ttgggcggct ggttcgacga actggtgatg cgggtcatcg
     4141 acgtgctgct cgcctttccg ggcattttgc tcaacattgc gatcgttgcg ctggtgccca
     4201 aagctggcgt cggagtgctg atttttgcgc tcgcggtaaa cggctgggtc ggctatgcgc
     4261 gggtggcgcg tggacaggtg ctgcgggtgc gcgagcagga atacgtgttg gcggcgcgct
     4321 ccgtcggttg tagcccgtgg cgggtgatgg cgcggcacat tttgccgaac atttggtcgc
     4381 cgatcatcgt gcaggcgacg tttgggctgg cgggcgttgt gctcgtcgaa gcgagcctga
     4441 gctttctggg gctgggaccg ccggtgccgt atacgtgggg ggcgctgctt gcgcagggga
     4501 cgaacttttt ttggcggacg cggcacttgg tgctggcacc ggggctttcg atcgcgcttt
     4561 tggtgctggg ctgcaacctg ctgggcgacg ggctgcgtga ctacgtggat ccaaaacgca
     4621 agcgggttcg ctaggtcgat ttgatggtcc agttgcgctc gatggtgatc atcggggcgg
     4681 cggactcggg gaagcggatc gacttgatga gctcgtttac gcagtcgcgg actttgtcgc
     4741 cttcaccctt gtcgacgttg acgctggtct ggatgttgat gacctgaccg cggccattga
     4801 tggtcacttt ggtgacgatg gcgacgctgt ttgcaccctt gtcgagcccg tggttgacgg
     4861 cgcagtcttg caccgccggg cctttggtga tgagcatctg ggtcagctct gccgccgtgc
     4921 cgccgccctt ggctttgcct ttggactttt tggcgacagg ttttcccgtc gctgctgggg
     4981 cttttgcggc ggtcggttcc gcagcatgag cggaaagcgg gagggacaag gccagcgccc
     5041 aagcagaaag tcccgatagc gctgcggcaa caagatgcga ggcgatcgga cggggggctt
     5101 tgcgaggcgc tgggaaatgg gcggacatca aactctcctt ccggctgttt gggccatttt
     5161 actttgcttt ggcagtgcgc ttccgaaaac cccttggcgt tgtgaatcgt accgctcgtc
     5221 gcgtcgcgaa gattgcaggc gcggccatgg ctaagctagt gtgcggccat ggctgccctt
     5281 cactcgcctc gttttcttcg tctgtgtcag caggcccagg ccgaaatcgt cgaggtgagt
     5341 gctgccgact ttgagcgtga gctggaaaac cgccaagcac ttctctcttc cggacgtgag
     5401 ccgccgccgc tttattttct cgatgtccgc gaagatcatg aatggctggc ggagcgcatt
     5461 cccggctcga cgcatcttgg ccgaggcatc ctcgaaagag acattgaaaa actgataccc
     5521 gaccccgacg cggacattgt gctgtactgc ggcggcggtt atcgctcggc gctcgccacc
     5581 gcgtcgctcg gcaagatggg gtacactcgc gtccgttcgc tcgccggcgg ttttcgcggc
     5641 tggaaggaat cgggccgggc gactgtgctc ggcgaaccgg agccttctgc gagccacttc
     5701 cgacaccacg ttattgaggg actgaccatg acaccgaaga acattgtcgt cgccaccgac
     5761 ctgactgagc ttagcgaacc cgcgctgcgg atggccaagt cactggccga gcagctgggc
     5821 agcacgatta cactggtcca cgtcctcgaa cccgcaccga cgccaccggg gctggaggcg
     5881 ttcgcgctcg aaggcatgcc gctcgactgg gaagaacggc ttttgcaagg tcgtagcgag
     5941 gcggcgagtc gacggctggc cgacaccgcc gcagcggaaa cttcggccaa ggtcaaggtc
     6001 cactggcggc ttctgcaagg tcggatgccc gacgcgctca tcgaggagct caaagcgatc
     6061 ggcggcgatc tgctgatcgt cggtacgcat gggcgcaaag gcgtctctca ccttttgctc
     6121 gggtcggttg ccgaaaagct gatgcgcggc gcgacttgtc cggtgctcgt cgtccgtccg
     6181 acggcgagct gagcgcgcgt ccacatggcg agggctgctt ttttcgacat ggacctgacg
     6241 cttttgcgcg tcaacagcgg ctctcgctgg gtcaagtacc tgcgcaagcg tggcgagatc
     6301 ggtctgccga tgctgattcg cagcggaatt tggctgctcc agtatcggct gtcgctgctc
     6361 gacatggaat cggtggcggc gatgctggcc aaggacatga cgggcgattc ggaagcggag
     6421 acgcgcacca aagtgctgga gttttatcgg acggnnnnnn nnnnatacca ggcacaaggt
     6481 ggccgaattt tatcgcaccg agctacagca cacgatttcc cccgacgcgc gcgcccagct
     6541 tgaccagcat cggcaggacg gcgatgcgat tgtactgctc acgagtgcga ccccgtatgt
     6601 ggccgagccg ctggccgcgg agctgtcgat tccccacgtc ctgtgtacgc ggcttgccat
     6661 cgaaaacggg cgcttttcgg gtcgggtgat gccgcccgct tgctacggca gcggcaaagt
     6721 gcaccacgcc gagcagtttg ccaaagcgca cggcatcgat cttgcccaga gcagttttta
     6781 caccgatagc tatagtgatt tgccgatgct gctgcgcgtc ggtcgaccgg tggcagtgaa
     6841 tccggatcgg cggcttttgc gctacgctcg tcgacaaggc tggccgactt tgcgctggta
     6901 gcgcgatacg cagggagaca aaccacgcat gaccccgaca ctgaaagtgc ccgacgcgca
     6961 ggttctattg ccacccgcgg tcgctcgact gattgacttg gcgctcgacg aggatctcgg
     7021 acgcggcgat gtgaccagcg aagcgatttt cggttcgcac gaaaccgcgc aagccgtcat
     7081 tttggccaaa agtgagctgg tgctgtcggg gctggacgtt gcgatggcgg tgtttcaagc
     7141 ggtggatcgg cggatcttga cgacggcgct gtttgcagac ggcagttcga tacatcccca
     7201 gacccaggtg ctgcgtgtcg aaggtccact gcgctcgatt ttggccgccg agcgaaccgc
     7261 actgaacttt ttgcagcggc tgtcggggat cgccacgctg tcccgtcgct atgtgcaggc
     7321 ggtggccgga acgtcggcgc ggatcgtcga tacccgaaaa acgctgccgg gcttccgttt
     7381 tctcgacaag cgggcggtgc gacacggtgg agcccacaac catcgggccg atctgggatc
     7441 gggcgtgctc atcaaagaca accacgtcgc ggcggcgggg gacgtggcgg aagcgattcg
     7501 gcgggcgcgt gaatatgcac cgcattcggt ccgcatcgag gcagaggtta cgtcgatcga
     7561 gcaggtgtct aaagcgctgg cggcgcgtgc ggacatcatc ttgctcgaca acatgtcacc
     7621 ggaaatggtg cgcgaggcga tggcgacgct gcccaacgtg ggggacccgc ggcgtccgct
     7681 catcgaagtt tcgggcggtg taagtctggc gacggtgcgg gcctatgccg aagccggggc
     7741 tgatctgatt agcgtcgggg cactgaccca ttcagtgacc gccgccgatc tttcactcga
     7801 agtcgaagtg tagtgagtcg ggggacgcat tgagcagcga gccgcacagt ttgacgcacc
     7861 aaagtccgtt tggcgaagag tcgctgggac cgggacggct gcggctcaag ctgacgatgc
     7921 ctgtgccact cgacggtggg cgactgtgtg atctgtcggt tgacatcgaa ggtcgtcccg
     7981 gcgattcgct tttgcatcgg cgtgggcgcg tcaagtccgc ggtcgtcgaa gtgagccagg
     8041 cgcagctgtc gcgggtgatt gcgacccagt tgccgtccct ttgtgcgcag cttcccgtcg
     8101 ggctggttgc gccggatcgt ctggcggtgc gcttttgcgg ttccgacgac aaaccgcagc
     8161 tctttgtggt ggcccgattt cccgacgctg gaccgcagcc gctgtggtgt tcgctgcgtc
     8221 tgccgctagg gcctgggcga caggatctgc ggggtccacg gcgcagcagc gagccattga
     8281 cgctgtcgct tggcctgtac gacgccgatt tgcgggtcta tggcttggcg cagcgatcgg
     8341 tggcactgct gggtttgtcg gcggcgattt gcgtcggtgc ggcgcttttg cgctttcgct
     8401 cggcgtcgac agaatcggtg gtgttgccag cgacggagtt tgcgcccggt ctggggatgc
     8461 ggctgttgtt cgcccacgga ctgcgcgaag tcggcgtcga cgtgctgtgg gattctctgt
     8521 tgcaggtgat gccgcgtcac ggccaacggg tggcggatgc gaaatcggca gcgctcgcgc
     8581 ttatcgaacg gcaagacggc ctcttgcggc tcagttacga aggcctgggc agcagctttg
     8641 attttgacga cgatccgcgt ggtcgctcga cggtagatgg cggacagatc gagtcgctgc
     8701 ttctggccga ccggctgctt atcgaaggcg atgttccagc ggcgctggcg gcctatgaac
     8761 tggttggtcg ggttgcgtct ttttccgacg ctgcagcact tcgtcgtgcg caaattttgg
     8821 cgacggtgcc aaagcggtcc gatgaggtct tgccgattct cgacggcctg gcagcgaaac
     8881 gcagcttcga catcgggacg cagctcttgg cgctggcggt gcgagtcgcg ggatcgcggt
     8941 cgtcgtccag tgcgctcgtg gcccagcgtg atgctttgtg tgcactggct cagtcggaag
     9001 aacacacggc ggaagaacaa gcgctgctga tgctgtgggc tgcgcagctt cccgtcggag
     9061 acaccgattc gctcggcctg gcccaaaagg cgcttgagag agtcagcgcg gacccaaact
     9121 gtccgacggc tgcgcttgtt gccgacggtg ccgcggcact tttgcaggcg ctttcgacgc
     9181 ggcttttgtt tgaatcgagc agtctgcccc cggcggtgga gtccagcgaa atctcgacgg
     9241 agctggcgct tgcggatcgc tgcttcgtcg cgggtgagct ttcgaccgcg gcggttcatt
     9301 accgtcgggc gctcgtcagt gatcaagttc caaaggctga gcttgcgcgc gtccacatgc
     9361 gacttgccga gatagcgcat caccacagcg acttggccga agaagaagcc gagctgggtc
     9421 ttgctgtcga gtgtggtggt ggggcggcgg catggtcagc gctcgcggca ctgtttcaga
     9481 gtttgggcga cggggcgcga cttggggttg cgctgtatgc gtggtctttg cacgaagtcg
     9541 gcgatgcgcg ggccgatttg ctgcgccagg cctcgcgtca cgtcggaccg agcttgctgc
     9601 tggcgatcga cgaagcgctg ttacagattg gcgctgacga cgagcttttg cgggaacggc
     9661 ttttgcgacg gcgcaaacag caggatgacc acgtcggact gctgagtctg ctgattcgcg
     9721 acatgcacca aagcagcggt gcgcgacggt gggcaagcgc tcgtctggct gcagacgtag
     9781 ccaggcggct gggcgatgtc gtcaccgagg ccgaagcgac gctgcttgca ctgtcggcac
     9841 cggcaaatcc gagtgtgacc gacgacgatg cgatgcgcgc gatcggtctt ttggctggcg
     9901 agcagcgaac gctgattccc cccgaaacgg tgtcgacgct gcggaatcga ctgaaagcgc
     9961 gcggttcgct gcggacggtg ctgcggcgtc tggatcagcg actggcgctg ctcgcatcgg
    10021 cgacggctcc gacggcttcg cttgtggagc tgtaccgtca gtcggcgctg tgcatgtcgc
    10081 ttctcgatga gtgggctgca gccgtggttc gctatctgcg tgccgccgcg cttggcgatc
    10141 gcacggtttt gcccgaagtt cgcaagctgc tggcgctttt gcttgccgac ggacagcacc
    10201 agcaggcgcg gcgtcttatc gaagcagagc tgcggcgttg tccgacggaa caagcagcgc
    10261 ctctgcgggt ggctctcggc gaggtgctgc tgcgcagcgg tcgtccgcgc gaagccttgg
    10321 gacagcttga actttcgctt ttgcgcgatg aagagctgcc cgtggcgcac gcgctgcttg
    10381 ggcaaatttt gcacggcctt gggcagccgg aagatcgtcc gcgggcgctt tcgcatctgc
    10441 tggaagcgag tcaatcttcg tcgctcagtc cagtggaaac gggggagtgt gcgctgcttg
    10501 cagcacagat gctgctcgcg agtgaagcga cggttcggct ggatgcggat ctggcggtgg
    10561 cgaatgcgcc gactttgccc aaagaagtgc aaaaacaggc cgagcggatg cttcagcggg
    10621 cgatcgagtt tttgcccaaa gatccgcgtc cgcgccggct gctgatttcg caccatgtgg
    10681 cgaaacaaca gcccgagcgg gcactttcgc tgtgtgacga gcttttggag cttgcgcaga
    10741 ccgatgccga gcgggccgac gttctgtggc aaaaaagtca gctggtacgc gacgagtccg
    10801 aagcggaggc gctgctgcag tcagcgcttt tgcacgacga aaaccacttg ccgacgctga
    10861 ccaggctgcg ggcgcgggcg gaacagcggg gtgacaaggc gacggcgctc agctggctca
    10921 gtcgagagct ggtcgcgagt ccgagcgaca gcgagcgggc gcttttgctg tgcaaacaag
    10981 ccgagctgct cgatccacag tcgcaagccg atctgatttt gacgacgctg cgcaaagcga
    11041 tgcagcttgg tttggggcaa gctgggcagc ggctggcagc gcttctgtgg gcgcgcggcg
    11101 atttgctgca agcggcggac attgcgggtc gggcggcgca gcttttgccg ccgtcggagc
    11161 aaggaaatca gctcttgctg gcagccgagt gggcgctgcg ggtgggcgac gagctgcggg
    11221 cacgcgaata tctgcgccaa gcgagcgagc agccgggcga gagcgcggaa gaagcggcgc
    11281 agcgcttggt ggttctcgac ggtggggatg agccggcgca tcggcggcgc acgctggaaa
    11341 atcgtctgac gcaagtgtcg acggggcttt cgagcatcga gacgctgcgc cagctgttga
    11401 tcctgtgtgc gcgacaaaac gatctggttt ccgtcgaacg ctatgcacag gcgctgcttt
    11461 cgcaggttcc tctcgatggg cttggtttga cggcgctgac cgattgcctg atagcgacgg
    11521 gccgatcgca ggaatcggtc tttccgcagc ttcagataag ccgggaatat ccgcgacgcg
    11581 ggccggtgct ggaagcgcgc gcagagtggc ttatcaaacg cggtgagtcg agcgaggccg
    11641 agtcgcattt gaccgaagcg ctggctcatg cggcgcgggg tccagagcgg ctgcggattg
    11701 gccagcagct ggcggatctg catcggcggc gaaatgcgca tggactggcg gcggcgacgc
    11761 tgagcgggct tctgtccgat gtcgatgagc gcgaagccag ggcggcgctg cgacgacgaa
    11821 ttgccgcgct gtatgtccag gccgggcagc cgctggtcgc gatcgaggag ctgcggtcgc
    11881 tgcttgcgga actaccgacg gaccttttgg cactcgaaca acttttcggg gtggccttgg
    11941 gttgtgacga tctgcaaagt gcccgcgcgt gtttggatca gcttgtcgcc cactcgacgg
    12001 ggacgatccg ggcgcgctgg ctttgtcgtc gtgccgagct gcaccagcgt caaggctcgc
    12061 tgtcggacgc gctgtcggat gccgaagcgg cgcttgccag cagccaagat ccggatttgt
    12121 tgcgtgcgct tttgcttttg ggtgtgcagc tttccgacgg tgggctgatt cggcagacga
    12181 cgatggcact gcatgcgcaa ggggcgccgt ttttggctgc gtcggcgctg gccggctgtg
    12241 ggctgctttt gcacgcgata ggtccgcccg aacaagccga gcggctgctt tcaccgctgg
    12301 aaacacctga cgagctggct ctggcgatgg cgcagtcggt cgcaggtttt cgcgggccgc
    12361 tgcatgatgt cgatcggctg ctcgacccgc tgcgtcgtcg gaagctgccg cggtttgaaa
    12421 cgctccgcaa actgctcgaa cagcgcgcgt tcggccagac cgtcgatttg ggggcgctca
    12481 agctgctcgc gcggctgacc gaggcggatt gggccccgct gcacgcgctg tatctatcgg
    12541 cgctggcttt tgtcgatccc ctcggggagc cggcgcagcg tctcgatgtg ctgccgaaag
    12601 tgcgtttttc cccgagtgag gagagactgc cggaaccaac agacgagctg cggccactgg
    12661 cgacgctgct gtcgcatttt gcgcggcttt tgctgcgcga taccagctca cgcgggggag
    12721 tcgatctggg accggtgcag cgggtgcggg aacgccaggc gcagctgttt gccaaaatgg
    12781 gtggcgagct ggtcggcgat ctcagttcct tgcgtgcact gttgggcgca gcgacggcgc
    12841 tgtggctgcc tggtcacgaa ccgaagaccg aagcagagcg gcagtggctg cgcagcttgc
    12901 aagcgcgggc gctgcttccg ggtcaaccgt ctttgacgac cgaacaagcc cacctcctgc
    12961 tcgatccggt gcggcatgaa ctggcgacgt cgcccgaaaa agtgacacag cagcttgggc
    13021 agactcgaga gctttttcac cgtcgggcgc tgctcttggc tgcggtggaa cagcacgatc
    13081 tgcgaccggc gctgtggtcg cttttgccag cgcatgaacc gatgggcgaa caaagccacg
    13141 gtccacaacc aggcgagccg accgagcccc acgatcgtct ggcgctgctg tcgcagccgc
    13201 cgctgcggac gctgcttctc gatgcgcagc ggctcctttc gtaatcactg cgcttttctc
    13261 ttcggcgcaa gaagtgcaat gatagaaagc ggaccatggc ggcttccgcg aacatgcagg
    13321 cgctcgtggg tacgacccag ggcaatacct acacactgga gcgactgctt tccgaagggc
    13381 gtcacggcgc gctgttcgag gccaagcaga ttcgactggg ctatcgctgt tttgtaaggc
    13441 ttcttacggt tgatccggcc cagcgcaagc cgctcctgac cgcactgagc cagcagacgc
    13501 agattccgca ccctgggctg tgtccgatcg tcgatacgat gcagctttcg gacgaaacgc
    13561 tcttgatcgc gtcaccgctt ttggccgggc aggatctggg ccagcgagtg gccgggcagg
    13621 gcaagctgtc gttgtcggaa ggaatggcgg tgctcagaac ggcggcgggg gcgctgcatg
    13681 cgctgcatca aaaagggctg tgtcacggcg gaatttcggg tcaaagcctg ctcattgttg
    13741 gttttgacga cgtcgcggtc gatggggctc tcgggggggg acaggtcaat cagcgggtgg
    13801 tgctgctcga cagcgggctg tatctggcgc agaaagagcg cgcttcggca gcggacgatc
    13861 aagccgcgct ggccaagctg attttggaca cggtgtcgga cttgtctccg cagctgcaaa
    13921 aagtgcttgg cactgcgcaa gatgcgtcgc agggaaggcg atacccgtcc atccaagcga
    13981 tgtggcaggc cgctgaagcg gcgcttggac gcaaagtcgt ggcggcccag ccgacggcgg
    14041 tggtgggcgc gctgaagctg ccgaaaatgg atgaacgagg ctcacgctgg ccgcttctgc
    14101 tggcgtcggc atcgtttctg atgttggcgg cggtgatcgg catcgtgggt ctgcgtcgtc
    14161 cggcgccgcc acctgattcg gccaagctgc tcaatcgggg cgctgtggaa accgatcaag
    14221 tgacgatttc gctcgagctg agtccggaaa ctgccaaggt gtgggtcgcc ggcaagctgt
    14281 tgcaaagtcc gctcaagctg ccgcgttcgt cgacggaagt gccgctgcgg attgaagcag
    14341 acggctactt gcctgcactg agcaaagtga tacccgaccg cgatcgctcg ctgcacatca
    14401 cgctcgccaa agcagcagag gcggcgcaag cgctggaggc agtcaaaaag ccccaccgct
    14461 cacgcagcaa aaaatagtca cggtcccgtt cgttacttcg ccggctgcgc gccatccagt
    14521 ccatcaagcg acggcaaatc cgagatgttg ggctgaagca cgatctcgat ccggcgattt
    14581 tggctgcggt gttccggcgt gtcgttggca gacaccggat cgaactcacc gaaaccggcg
    14641 gcagccagca cctgcggctt cattcccttg gacacaagaa acagcgtcac ttcaatggcg
    14701 cgggccgccg acagatccca gttcgatcga taccgtccgc cacgaatcgg cacgttgtcg
    14761 gtgtgtccag cgacgacaaa gcgccgatcc ggaatcgtcg ccagaacctg cgcaatcttg
    14821 cccagcgctt cttggccttg cggcttgact tcggctttgc ctgaatcaaa cagcacgtcg
    14881 tcgggcagcg cgatcagcat ccgtccttct cgcgtcacca ctttcagttg gcctgcatcg
    14941 atcatcgcgc gcagcttgtc gacgagacta cggaacatct gaagccgggc ttccgccgcc
    15001 gctttctgct tgcgcagctc ttccaagcgg gtcttggcat cggtcaggcc ggcttgaagc
    15061 tgtccgcgct cggtcgtcag cttgtcgacg ttctgcccca gcttttccag ccgctctttc
    15121 agctgtccca cgagcgccgt cgagtcattg agcagccgcg catactgatc gcgctcggtc
    15181 gcaagcccct tggctttgct ttccgcctgg ttcagccgtc ccgacagatc gccgacttgc
    15241 gccagcgcgt cttggattcg ctttttcagc tcgtcgcgct ctttgttgag gtcgacgttg
    15301 tcctgccgac tcttggccag atcggcttgc gcacgggcca gctcatcgag cttggcttga
    15361 tgtgttcccg ccgtcacaca gccactcaga agcccaagtc ctagcagcgc gccgatcgtc
    15421 caatttcgca ccatggtgtc tgtctcccgt caaaaacgac tcgcagcggt atatagcacc
    15481 acgggcggca gcgcgctttt ggtcctggca caagctgcga attttctcga aagtgcgccg
    15541 ttcacttggg acaaaatggc cgcgtgcgga gctgggtctg ggttttcagc ctgttgtgcg
    15601 gatgtgtcgt ctacgaccag gatgtgctct ggcagacgga cgtcgccgct gtgccgtcag
    15661 accttgcgca gacgagcagc gacttgagca ccccgatgtg tggttggcag gaactgtcgt
    15721 cgatgtgcaa agagcacagc gcctgggtca gtgcggcggc gcgagcctgc tgtgatttgt
    15781 ttgggcagca gcttcgctcg ctccagtttg gttcggtttg tccacagatg ggttcgtttg
    15841 gtgcgcgcca ggttcggttc gagtgctgtc cgtctgacga ccccaatccg acgacgcact
    15901 gcgagacccg caagctcggt tttggcgaat gtctggatga cgccatttgg aagcagcgcg
    15961 ccgcgacgga atgtggcaac agcgggcgga tcgtatcgtc gacgagccag cgcttgtcgt
    16021 cgccctgcgg ccctggtcgc tattccggca tcgtatttga ctgctgcacg attccctgtc
    16081 cgacgccgat tacgcgctaa ggttgggctt ttggcgcggc agattgcgac tgcgcagcgc
    16141 cagcgcgtgc tcgctcatcg ggacaacgcg gaagtgttgc gccagttttt cgagcacctc
    16201 gacgagcaga tcgcgctttt gggaagacgg catgttcatc gccggaaaga agcgcagcgc
    16261 gtcgcactct ttgccggaaa tgaagtcgag cggatgcagc aaaaacgacg gctcgacaga
    16321 acgcagcttg caaagctgga tcgccgtatc gagatagcga cgcatcacaa acggcg
//
LOCUS       DAORGN010000106        16317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1457_length_16317_cov_5.439614, whole genome shotgun sequence.
ACCESSION   DAORGN010000106 DAORGN010000000
VERSION     DAORGN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16317
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_16317_cov_5.439614"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1980)
                     /gene="speA"
                     /locus_tag="PKL17_12595"
     CDS             complement(52..1980)
                     /gene="speA"
                     /locus_tag="PKL17_12595"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNK45619.1"
                     /translation="MHSTGSIPRRWSIADSRETYAIHKWGGPYFDIGDSGNIIAYPGN
                     GSGESSHGIDLKELVDEVRERGIGLPLLIRFPEILKSRITELNQAFGSAIAEYGYKGK
                     YRGVYPIKVNQDRFVVEQIVQYGRPFHYGLEAGSKPELLTVISMLEDEEALIICNGYK
                     DEEYVESALLASKLGRNIILVVEKFSELPLIATIARRNNIRPRIGIRAKLATRGSGRW
                     EASGGDRSKFGLSSREMLEAMRYLADEGLIESFELLHFHLGSQISAIRSIKNAMREAG
                     RFFVELSKMGAPLRYLDVGGGLGVDYDGSQTNFSSSMNYSLQEYANDIVYSLMELCDA
                     AGIAHPTIVSESGRAIVAHHAVLVVDVLGVSEFAVGKVPESLPDNAAQVVRHLFDSYR
                     DVTRKNFLEIYHDAIEYKDECLTLFALGHLSLADRVIAEDIFWALCQKVLRILRDMPH
                     VPEDLEGLEHALSDTYFCNFSMFQSLPDSWAVDQLFPILPIHRHSEEPTRRGVLADIT
                     CDSDGKIDQFIDPREVKNVLELHPLTEGDYYIGMFMVGAYQEILGDLHNLFGDTNTVH
                     VSCASGGGYHIEHVITGDTVTDVLKYVGYSRDELTARLRRSVEHALRNRRMTLEESRN
                     LLRIYESGLSGYTYLERD"
     gene            2211..2948
                     /locus_tag="PKL17_12600"
     CDS             2211..2948
                     /locus_tag="PKL17_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HNK45620.1"
                     /translation="MADRSIPISEHTVREVALAMPVAVPVFAHVGIEFGCGGDVRIVD
                     ACREIGLSVEALWALIEESAKQPVPGDLEFWHNAAPSALMDHIVHRYHEPVRKELQRL
                     ESLSAKVAQRHGEAFPQVLQIQRLIASLHRDMELHMVKEERVLFPLIKRLDATLGRGM
                     TDRSAANQLLPPLRLMLTDHDGIMNFVRALRQQSDGYSVPDGVSASYHMLCSGLRGLQ
                     YEIQIHLHLENNVLFGAARDMLVACGL"
     gene            3004..4563
                     /locus_tag="PKL17_12605"
     CDS             3004..4563
                     /locus_tag="PKL17_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK45621.1"
                     /translation="MSRSEVGGRALFDPQSLPVLVVDDEADNLDAFRFSFRKGFRILT
                     AQSAEAGLQILSQEKVAVIVADQRMPKMTGLQFLETAQRLVPLAVPIIVTAYTDVDVL
                     IEAINSGHIYRYITKPWDSKELRGVLLQALERYHLLVENARLQKQLEHYAGYLEKELH
                     GAADFGQLVGESAALRAALLRVEQVARTDATVLLRGESGTGKELVAHAIHINSRRSNR
                     PFVRVNCAALASGVLESELFGHERGAFTGAMARHPGRFELADGGTIFLDEVGDLPMEV
                     QVKLLRVLQERSFERVGGSETLHVDVRVISATHKDLEAMIAAGTFREDLYYRLNVFPI
                     VLPPLRDRLEDLPMLCQHFLHKWRKHAAQRVTQVSQQALAYLAQYDFPGNVRELENLI
                     ERALILAVGSEITPAELEFVRPSSRSGISTGSPSRPPASAPVVVASPPPPSAPAPNAP
                     TEPLSRTLSDRLLDQERAEILSAIERADGNIAQAARLLGYNRSTLYYRLRKHDLLHLL
                     PTKPELLAPLP"
     gene            4573..5133
                     /locus_tag="PKL17_12610"
     CDS             4573..5133
                     /locus_tag="PKL17_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45622.1"
                     /translation="MEPTFPALLWAVCCLWIFTGIAGVDGLYFHLYRYRLYSRPASMY
                     EHKLHTVNAVLFVPQVILLFLFQARGLWLWLLLGLFLTTVAVEITDVLCEEDSRRDIG
                     GLTSLEYLMHFLMAGLRFGWVVPLLFGLPSEAWSPATSSLIFRSLWMLIAGGWIAGPA
                     TIIAVIHVILALRTYPPDAQAKPAAG"
     gene            5158..5760
                     /locus_tag="PKL17_12615"
     CDS             5158..5760
                     /locus_tag="PKL17_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45623.1"
                     /translation="MMRIVSARRKNRPVRPLPASNLPGSPRSFFCVDLRSVENHFYQK
                     PVDELRQLCRGSRLGALLATLDLDPRHPLGKNFQRLPTGQAFALFDALCEELAESVLF
                     DVPHVAWQPLLQLLLPIDGGESPYPKELHAAVGLESDEGLLLLLEGRLAAEAATVALQ
                     PSLLPSGSGLDPLLWSDVRHALLPMLESVRDKSRALLMER"
     gene            complement(5761..7344)
                     /locus_tag="PKL17_12620"
     CDS             complement(5761..7344)
                     /locus_tag="PKL17_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK45624.1"
                     /translation="MSAVVEGRSLCGPSAPLALSADVVIVGSGASGAVVAAELSEAGQ
                     KVIVLEEGPHLLPSQLATMRPSETMRHGWREAGMSFTVPVGDSPAINLMVGRCVGGSS
                     VMTGGVCFRTPDHILHEWVSDLGLRALNPSAMTPFFEEVERRCHIELVPEHMRSRSTV
                     LYGQGAEKLGYKLAPNHRNTKDCDGCSRCNFGCPHGAKWSVDLTYLPRAQAAGAEVFS
                     DALVEKLILRGDTATGVSGRFLDADGKPGRRFIAHARRVVLACGGMHTPRLLQDSGIS
                     DRLSSVGEGMTLHPSMRVMARFDEPVHGWRGALQSAHSEAFIDEKITLMSVFVPSGVL
                     AATLPGIGQSHKRRAEMAPYLAVFGGMVHDDPGGRVLSRRSPLGMLWGRSEPAVTYRM
                     SRRDRQGISRMLRILADTFFAAGAREVFLPILGGPPGFPDGGITADDLRGIDLDKIPS
                     QRFECASQHPLGSCRMGLSPDDAVVDEQGQVFGLRELFVVDSSILPTSLGVNPQVTVM
                     AMATRLAWQLRERRLPEAA"
     gene            complement(7341..7772)
                     /locus_tag="PKL17_12625"
     CDS             complement(7341..7772)
                     /locus_tag="PKL17_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45625.1"
                     /translation="MTSSKLPRTERAIRLFAETLFSDLDGPPPPDRIARLAQDATALV
                     SATTQRARLLFQLCLFAVLWAAPLFVFRAPGLSRLPLAGRVAALESMESSPTGAALVL
                     AIKAVLCVLWYEQPENHAAIGGDPFCQPGHRRLPILQGAKT"
     gene            7896..9116
                     /locus_tag="PKL17_12630"
     CDS             7896..9116
                     /locus_tag="PKL17_12630"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNK45626.1"
                     /translation="MRAQYPVWFGLLRRGSVGAICVGLATTVLAGCSNPESGGKLNCP
                     PGMRTVWHDPLNGVGECEIVTPLSDGHTPRSGLEQPFPAMNRPSGALTTPERIELGRL
                     LYFDPVLSGDNTVSCAHCHHPAYGFTDNRPVSTGIGGKQGGRSAPTVWNAMWNSEQFW
                     DGRVKTLEDQAKGPIQNPIEMNQDPVKLVGELQAIPEYRQRFDEAFRGSNGSAVTLDN
                     IALAIAAFERTIASVSSPFDRYAAGDRSAMNESAQRGLNLFRSVSTRCFECHEIPTFA
                     MKEYKVLGVPSLANPTMVDDDRGRAAIMPGAAYERAFRVPTLRNITKTAPYMHNGSFK
                     TLDEVLDFYAKGGGLGRGYKLDNVDDKIRKFDLSADEKADLIAFMEALTDESLVPEVP
                     KTVPSGLPVAKGPL"
     gene            9182..10360
                     /locus_tag="PKL17_12635"
     CDS             9182..10360
                     /locus_tag="PKL17_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNK45627.1"
                     /translation="MSKATTLFCLASLSGFLLSGCAERPLYDCYMGAQNADERRDCIA
                     SGKADATVAPGQSITQAVFDMPADATRWVIKVMPGTYRETVSVDRSGVMLVGVTYADK
                     PDERVIIDGGLDGGKFRKDAVIVSGSRFVMDGITMRGFSGNGVTTSKSSGVTLRNLVA
                     DKTGHYGVYPVESEDILVEDCVTTGINDAGIYVGQSRRATVRHNQVFGNVAGIEIENT
                     VTALVEENDVHDNTAGIMAFVLPQAPSKEGRDCTIRNNKSYRNNLKNFADPNAFVAKL
                     PYGIGIGVMAADGTVVEKNEISDNSSTGVALIGLQLFFSDPTKLDVEPNTDRARVTNN
                     TYRGNGKAPDPAYVNGAPVKLNGADIMWDGTGTGNCVDEPQGGELKTDAAASALPRCK
                     "
     gene            complement(10376..11176)
                     /locus_tag="PKL17_12640"
     CDS             complement(10376..11176)
                     /locus_tag="PKL17_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK45628.1"
                     /translation="MSILSPSVAVVTGANRGIGAEIARQLAHKGFLVFVGCRQLHDGD
                     VVASSIRAAGGQARALALDVSDPASILAAVESVSAQVGQIDALVNNAGIALEGFNLDI
                     VRRTLAVNFYGAERATFAFLPLLARHGKVVMLSSGMADRSALGPSLRTRFSTPLSRAE
                     LHALLADFEQAVAMGQHRAHGWPQSAYRVSKLALNVLCETLSRELADDPRDLVINACC
                     PGWVKTDMGGPSADRSVEEGADTPVWLATLPADGPRGGFFRDRAVASW"
     gene            complement(11237..11818)
                     /locus_tag="PKL17_12645"
     CDS             complement(11237..11818)
                     /locus_tag="PKL17_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45629.1"
                     /translation="MNRSQNVIPLLLASGVLFGAPDAQASFYPPSYVIETTCKSEGPV
                     EVCAQNQHYGMPQLVVRYRGSLLATQWGRISAYVKLNGRDGTFRMQNASFEESVRLGD
                     PQEEYICSIADSNNPDWKPSPPARLCPLPAKPGGGTTWYYVPAPDAERALFFYARDSF
                     GSANNWDIELAFVADDGSWDSKYGANYRFTFVR"
     gene            11955..>12752
                     /locus_tag="PKL17_12650"
     CDS             11955..>12752
                     /locus_tag="PKL17_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNK45630.1"
                     /translation="MRKTIWALAGLLGIAPAGIARADFSPEYELGVFGGAHLWNPLGG
                     VGRPDDSEASQVEHGGLLGVRLGLGLHTRFALEAELGLAPSFTKGLASANDPTFDVSA
                     RVLGIGYRIQGLVHILTGRFRPYALIGIGGFTTTSSSAALFGQDTTYSASVGGGLKVD
                     LGKNWGLRVDGRALMHKGLPNGPAIAPDGEVSLGLFGRFGDVKQTASSIGVADRDKDG
                     ITDGADLCPDVPGIPALRGCPQPAGVLVPPSAVPPANTSTVTLPSLPP"
     assembly_gap    12753..12775
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12810..13085
                     /locus_tag="PKL17_12655"
     CDS             12810..13085
                     /locus_tag="PKL17_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HNK45631.1"
                     /translation="MTQRFCDKSGEPLSLVLYMFDECPFCQRVLSAMGALGFDLPMRN
                     IRKDLSARDELIRVGGSKQVPCLFINGKPLYESADIIDYLKAEVRRA"
     gene            13183..13842
                     /locus_tag="PKL17_12660"
     CDS             13183..13842
                     /locus_tag="PKL17_12660"
                     /inference="COORDINATES: protein motif:HMM:NF021360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
                     /protein_id="HNK45632.1"
                     /translation="MAMQNLRDKLLKAGLVDKKQKVQVDTQDRREKKQRGADQLAAEE
                     AEKQRLFAEKQAAEAEARRQADLAKVAERASHEAANRVRNICDRWAVRLTKPGQRRFY
                     FVQRSGRVGYLWLPEVICDQLCLGALAIVERLPTEETTDWASRPRIKGRKPRSLLQKL
                     VSQVKKGEMQFVSEETHVLLPPEPADRILAIDASAVRFWARSGKPLGYVAEAGDSAGA
                     A"
     gene            complement(13803..14771)
                     /locus_tag="PKL17_12665"
     CDS             complement(13803..14771)
                     /locus_tag="PKL17_12665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNK45633.1"
                     /translation="MKPLLWKFVLPLLGDVTFPAYFTFLTLGLFFAAVLTIRESRKLD
                     LDPDDIIDINLYMTIFGVIGSRILHVFVDGHLQDYVNLCIDPLKVPAIDALVKHCNTA
                     RECGYDYLCDTTRHLCYPPRDCFAWAKLWRGGLVYYGGLILATAYAVYYTRRANIRFL
                     RVADLAAPAILLGLFFGRLGCYLNGCCYGKITNSLFGVRFPIGSSPWRAQYEAHQIMA
                     GQQMLPVHPTQLYESLGCLLLFAVTYFWVRPRKRQEGFIAGACLALYAILRVVVEVWR
                     DDERGVLMGFLSTSQIISLFGMALALWLLYRRDPYAAPAESPASAT"
     gene            complement(14778..15356)
                     /gene="lspA"
                     /locus_tag="PKL17_12670"
     CDS             complement(14778..15356)
                     /gene="lspA"
                     /locus_tag="PKL17_12670"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNK45634.1"
                     /translation="MKSKYIAFLLLFLVSLGLDQSTKIWARQTLKPANYPNAISAPKT
                     VIKGYWDFRYSENPGSAFGMFRNQAGARYFLFGIGIVCLGVIGLWLYRLPPNAKWLGV
                     KLGLLAGGAIGNMVDRAMYSRVTDFILWKITTSSTTYEWPTFNVADAALVIGVGMILI
                     DRPRDPLPPEESQPASGETAPAGPTAEKPASA"
     gene            complement(15437..>16317)
                     /locus_tag="PKL17_12675"
     CDS             complement(15437..>16317)
                     /locus_tag="PKL17_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNK45635.1"
                     /translation="IDLLSQVGPPAGAPDEFLLLTIDDEDDCVYAELPQGLTRPLRLT
                     AAEAFSLTLGLRALAGSGVRPYEDAVARLLAKIRAALGEASPELLALERESVVETEDH
                     HKLSLLSDLHRAITARRPLTAKYHSASRGQTEQRRIDPYALLNHRGSWYLVGRCHRHN
                     EPRLFKVERLSNVVIHSDVPSFVVPGDFDLEKFRRDHLFQPEAAHPRIELRFDKTLAY
                     EVRTRFDPARVQVARDGSLQVTIENPLSDWLVSWILGFGAGVEVQEPAELRQKVAERA
                     QAIAAHHDQVATHTAA"
BASE COUNT         3176 a         4905 c         5062 g         3151 t
ORIGIN      
        1 ctgctgccga gcccgctacc gtcggcaatc tgcgggaatt agggacggcc cctagtcgcg
       61 ttcgagatag gtgtagccag agagtcccga ttcgtagatt cgcagcaggt tgcggctttc
      121 ttccagtgtc atgcgccgat tccgcagcgc atgttccacc gaccgccgca gccgcgccgt
      181 cagttcatcg cgcgaatagc ccacgtactt gagcacgtcc gtcaccgtgt cacccgtgat
      241 cacatgctcg atgtgatagc cgccgcccga cgcgcacgaa acgtgcaccg tgtttgtgtc
      301 accaaacagg ttgtgcaggt cgcccaggat ttcctggtat gcgccgacca tgaacatccc
      361 gatgtagtaa tcgccttcgg tcagcgggtg cagctccagc acatttttga cttcgcgcgg
      421 atcgataaac tgatcgattt ttccatcgga atcgcaggtg atgtctgcca gcacgccgcg
      481 ccgcgtcggt tcctcgctgt gtcggtgaat cggcaggatc gggaacagct gatccaccgc
      541 ccatgagtcc ggcagcgact ggaacatcga aaaattacag aagtacgtgt ccgacagcgc
      601 gtgctccaga ccttccagat cctcgggcac atgcggcatg tcgcgcagaa tccgcagcac
      661 tttttggcac agcgcccaga agatgtcttc tgctatcacg cggtcggcca gcgacaagtg
      721 acccagcgcg aacagcgtca ggcactcgtc tttgtactcg atggcatcgt ggtaaatttc
      781 gaggaagttt ttccgcgtca catcacgata ggaatcgaac aggtgccgga ccacttgcgc
      841 ggcattgtct ggcagcgatt ccggaacctt gccgacggca aactcgctga ccccgagcac
      901 atcgaccacc agcacggcgt gatgggccac aatggcgcgt ccggattccg acacgatcgt
      961 tggatgcgcg attccggctg catcgcacag ctccatcaag ctgtagacga tgtcgtttgc
     1021 atactcttgc agcgagtagt tcatcgacga gctgaagttg gtctgcgagc cgtcgtagtc
     1081 gacgcccaga ccgccaccca cgtcgagata acgcagcggc gcgcccattt tcgacagctc
     1141 gacaaagaac cgtcccgctt cgcgcatcgc atttttgatc gatcgaatcg ccgaaatctg
     1201 gctgcccagg tgaaagtgca aaagctcgaa gctttcgatg agcccttcat cagccaggta
     1261 gcgcatcgct tcgagcattt cacggctcga cagaccgaac ttgctgcggt cgccgcccga
     1321 tgcttcccag cgtcccgaac cgcgcgtcgc cagcttggcg cggatgccga tccgcggacg
     1381 gatgttgttg cggcgtgcaa tggtcgcaat cagcggcagc tcggaaaact tttcgaccac
     1441 caggatgatg ttgcggccca gcttgctcgc gagcagcgcg ctttcgacgt attcctcgtc
     1501 tttgtagccg ttgcagatga tgagcgcctc ttcgtcctcg agcatcgaga tgacggtgag
     1561 aagctctggc ttgctgccgg cttcgagtcc gtagtggaaa ggccgcccgt actggacgat
     1621 ctgttcgacg acgaagcggt cttggttgac cttgatcgga tagacgccgc ggtacttgcc
     1681 tttgtagccg tactccgcga tggcgctgcc gaaagcttgg tttagttcgg tgatccgcga
     1741 cttgaggatt tccggaaagc gaatgagcag cggcagaccg atgccacgct cgcgcacttc
     1801 atcgaccagt tctttgaggt cgatgccatg gctcgattcg ccacttccgt tccctggata
     1861 cgcgatgatg ttgcccgaat caccgatgtc aaagtacgga ccgccccact tgtggattgc
     1921 gtaggtctct ctcgaatcag cgatggacca gcggcgagga atcgaaccag tcgagtgcat
     1981 ctatcggaac tccttcgtcg gcctgtcctc ggcagcgtcc ctattgacgc gcgcggaaga
     2041 ccgaataaac gggccgcagg atgaacgcac ggtcccggtc tcgcaaccaa aaaatgcggc
     2101 cttcggaccg tccaaaaacc gccagtcgca cttggtcgat cgaagccgtg aatgtggcgc
     2161 ttccttcgca agttcactga tacactagtc gcaaactttc ggggggaacg atggcagatc
     2221 ggagcatccc gatcagcgag cacacggttc gcgaagtcgc gctggcgatg ccagttgcgg
     2281 tgccagtgtt tgcacacgtc ggcatcgagt ttggctgcgg cggtgatgtc cgcattgtcg
     2341 atgcctgccg agagattggc ttgtccgtcg aagcactgtg ggcactcatc gaagaatcgg
     2401 ccaagcagcc tgtcccgggt gacctggagt tttggcacaa cgcggcgccg tctgcgctga
     2461 tggaccacat cgtgcatcgc taccacgagc cggtgcgcaa agagcttcag cggctggagt
     2521 cgttgtcagc caaagtcgca cagcggcacg gcgaagcgtt cccgcaagtg ctccagattc
     2581 agcgcctgat cgcgtctttg catcgcgaca tggaactgca catggtcaaa gaggagcggg
     2641 tgctgtttcc gctcatcaaa cggctcgatg cgacgctcgg gcggggcatg accgatcgct
     2701 cggcggcgaa tcagcttctg ccaccgctgc ggctgatgct gaccgaccac gacgggatca
     2761 tgaactttgt gcgggcgctg cggcagcaga gcgacggcta cagcgttccc gacggtgtat
     2821 cggcgagcta tcacatgctg tgctccgggc tgcgcggtct tcagtacgaa attcagatcc
     2881 acctgcacct cgaaaacaac gtgctgtttg gtgcggcgcg cgacatgctc gtcgcctgcg
     2941 gtctgtaaac ggcaggcacg tcgcctcttt acggcgagtg acggcgctgc tacggtcgcc
     3001 gccatgtctc ggtcggaagt gggtggacgt gctctttttg atccgcagtc gctgccagtg
     3061 ctggtggtcg atgatgaagc ggacaacctg gatgcgtttc gctttagctt ccgcaaaggc
     3121 tttcgcattc tgaccgcgca gtccgcagaa gccgggctgc aaatcctgtc gcaagaaaaa
     3181 gtcgcggtga tcgtagcaga ccagcggatg ccgaagatga ccgggcttca gtttttggaa
     3241 actgcgcaac ggcttgtgcc gctcgcggtg cccatcatcg tcacggcgta cacggatgtc
     3301 gacgtgctca tcgaggcgat caacagcggc cacatctatc gctacatcac caagccgtgg
     3361 gacagcaaag agctgcgcgg cgtgctcttg caggcgctgg agcggtatca ccttctcgtc
     3421 gaaaatgcgc ggctgcaaaa gcagctggaa cactacgccg gctatctgga aaaagagctg
     3481 cacggcgccg cggactttgg tcagctcgtc ggtgaatctg cggcactgcg ggcggcgctg
     3541 ctgcgggtcg aacaggtcgc tcgcacggat gccaccgtgc ttttgcgcgg cgaaagcggc
     3601 accggcaaag agctggtcgc gcatgccatc cacatcaatt ctcgacgcag caaccggccc
     3661 tttgtgcggg taaactgcgc ggcgctggct tccggcgtgc tcgaatcaga gctgtttggt
     3721 cacgagcgcg gcgcgttcac cggcgcgatg gccaggcatc cggggcgatt cgagctggcc
     3781 gacggcggca cgatttttct cgatgaagtc ggcgatctgc cgatggaagt ccaggtcaag
     3841 ctgctgcggg tgcttcaaga gcgcagcttc gagcgtgtcg gtggcagtga aacgctgcat
     3901 gtcgatgtgc gggtgatttc ggcgacgcac aaggacctgg aagcgatgat tgcggcgggc
     3961 acgttccgcg aagacctgta ttaccggctg aacgtgtttc cgattgtgct gccgcctctg
     4021 cgcgaccggc tcgaagactt gccgatgctg tgtcagcact ttttgcacaa gtggcgcaag
     4081 catgcggcgc agcgcgtgac ccaggtttcg caacaagcgc tggcctatct tgcgcagtat
     4141 gactttcccg gcaacgtccg cgagcttgaa aacctgatcg agcgggcgct gattctggcc
     4201 gtcggcagcg agatcacacc ggcagaactg gagtttgtgc gtccgtcgtc ccgatccggg
     4261 atatcgaccg gttcaccgtc gcgcccccct gcctcggcgc cggtggttgt ggccagcccg
     4321 ccaccgccgt ctgcgcccgc gccaaatgcg cccaccgagc cgctcagtcg aacgctgtcg
     4381 gatcggctgc ttgatcagga acgcgcagaa attctgtccg cgatcgagcg tgccgacggc
     4441 aacatcgcgc aagcggcccg gctgctcggc tacaaccgca gtacgctgta ttaccggctg
     4501 cgcaagcacg accttttgca tctattgccg acgaagccag agctgcttgc gcccttgcca
     4561 taaggtctga acatggaacc cacttttcct gcgctgctct gggctgtgtg ctgtctgtgg
     4621 atttttaccg ggatcgcggg tgtcgacggg ctgtactttc acttgtatcg ctatcggctg
     4681 tattcgcgac cggcgtcgat gtacgaacac aagctgcaca ccgtaaacgc ggtgctgttt
     4741 gtgccgcagg tgatcctgct gtttctgttt caagcgcgcg ggctgtggct gtggcttttg
     4801 ctcgggctgt ttttgacgac ggtggcggtc gaaatcaccg acgtgctgtg tgaagaagac
     4861 agtcgacgcg acatcggcgg gctgaccagc ttggaatacc tgatgcactt tttgatggcc
     4921 ggactgcgct ttggctgggt ggtgccactt ttgttcggtc tgccgagcga ggcgtggagc
     4981 ccagcgacaa gctccctgat ttttcgatcg ctgtggatgc tgattgccgg gggttggatt
     5041 gccgggccag cgacgatcat tgcggtgatt cacgtcatct tggcgctgcg cacctatccg
     5101 cccgatgcgc aggcaaagcc agccgctgga tagcgcattc cccgtggtaa gatggcgatg
     5161 atgcggatcg tatctgctcg tcgcaagaac cgtcctgtaa ggcccctgcc ggcgtccaac
     5221 ttgccgggct cgccccgttc gtttttttgc gtcgatctgc gcagcgtcga aaaccacttt
     5281 taccaaaaac cggtcgatga gctgcgccag ctctgtcggg gcagtcggct gggtgctctg
     5341 ctcgcgacgc tggatctcga tccgcgccat ccactcggga aaaatttcca gcggctgccg
     5401 acgggacagg cctttgcgct gttcgatgcg ctgtgcgagg aactggcgga atcggtgctg
     5461 ttcgatgtgc cgcacgtcgc ctggcagccg cttttgcagc tgcttttgcc aattgatggc
     5521 ggggaatcgc cgtatcccaa agagctgcac gctgcggtcg gtctggaaag cgacgaaggg
     5581 ctcttgctgc ttttggaagg gcggctggct gcggaagcgg cgacggttgc gcttcagccg
     5641 tcgcttttgc caagtggcag tggccttgac ccgctgttgt ggagcgatgt ccggcacgcg
     5701 cttttgccga tgctggaatc ggtgcgcgac aaaagccgtg cacttctgat ggaacggtag
     5761 ttacgccgct tcggggagtc gccgctcgcg cagctgccag gccagccgcg ttgccatcgc
     5821 catcaccgtc acttgcggat tgacccccaa gctggtcggc aaaatgctgg aatcgacgac
     5881 gaacagctcg cgcaggccga agacctggcc ctgctcgtcg acgaccgcat catcaggaga
     5941 cagacccatt cggcacgaac cgagcggatg ctgcgacgca cactcgaagc gctgactggg
     6001 aattttgtcg aggtcgatgc cgcgcagatc gtccgctgtg atgccaccat cgggaaaacc
     6061 cggcggccca cccaggattg gcagaaacac ctcgcgtgcg ccggctgcaa aaaacgtgtc
     6121 ggcgaggatg cgcagcatgc ggcttatgcc ctgtcgatcg cgtcgcgaca ttcggtacgt
     6181 caccgccggt tcactgcgtc cccacagcat tccgagcggc gagcgacgcg acagcacccg
     6241 accacctgga tcgtcgtgga ccatgccgcc aaagaccgcc agatacggtg ccatttccgc
     6301 ccggcgcttg tggctttgac caattcccgg cagcgtcgcc gccagcacac cgcttggcac
     6361 aaagacgctc atcagggtga ttttttcgtc gataaacgct tcggaatggg cactttgcag
     6421 cgcgccgcgc cagccatgga ccggttcgtc aaagcgcgcc atcacccgca tgctcggatg
     6481 cagggtcatc ccttcgccga cggagcttag ccggtccgaa atgccactgt cttgcaagag
     6541 ccgcggcgtg tgcatgccac cacacgcgag cacaacgcga cgggcatggg cgatgaatcg
     6601 acgaccgggc ttgccatccg catcgagaaa acgcccgctt acccccgttg cagtgtcgcc
     6661 gcggaggatc agcttttcga cgagcgcatc ggaaaacact tccgccccag cggcctgggc
     6721 gcgcggcagg taggtcagat cgacgctcca tttggcgccg tggggacagc cgaagttgca
     6781 gcgcgagcag ccgtcgcagt ctttggtgtt gcggtggttt ggcgcaagct tatatcccag
     6841 cttttcagcg ccttgtccgt agagcaccgt cgatcgcgag cgcatgtgct cgggcaccag
     6901 ctcgatgtgg cagcggcgct cgacttcttc aaaaaacggc gtcatcgcgg acggattgag
     6961 cgcccgcaga cccaaatcag aaacccactc atggaggatg tgatcgggcg tgcgaaagca
     7021 gacgccaccc gtcatcaccg atgaaccccc gacgcagcga ccgaccatca aattgatggc
     7081 tggactgtca ccaacgggaa cagtgaacga catgccggct tcccgccagc cgtgccgcat
     7141 ggtttcagac ggacgcatgg tcgcaagctg cgatggcaga aggtggggcc cttcttccag
     7201 cacgatgacc ttttgtcccg cttcggaaag ctccgctgcg acgacggcac cgctcgcccc
     7261 cgatccgaca atcaccacgt cggcagacag cgcaagcggc gcacttgggc cacaaagact
     7321 acgtccttcc acgacggcac tcatgtcttg gctccttgca aaatgggcag ccgcctgtgg
     7381 cctggctggc agaacggatc gccgcctatc gccgcatgat tttccggttg ttcgtaccac
     7441 agcacgcaga gcaccgcttt gattgccaga accagtgcgg cccccgtcgg actgctctcc
     7501 atcgattcca gcgctgcgac ccgtccggcg agcggcagtc gtgaaagccc cggtgcgcga
     7561 aaaacaaaca gcggcgcggc ccacagcacg gcaaacagac agagctgaaa caaaaggcgg
     7621 gcccgctgtg tcgtcgcaga aaccagtgcc gttgcatctt gggccagccg ggcgattcgg
     7681 tccggcggcg gcggtccgtc aagatcggaa aacagcgtct cggcaaacag ccggatggcg
     7741 cgttcggtgc gcggcagctt ggacgaagtc atgcgcggaa gaatgcccaa tcgacgccga
     7801 tccacgcaag ccccacgacg gcaatcgccg ctgcacgttc gctttggcgg caaacggttg
     7861 cacattgtgg ggctgcgatc gtagcttccc gatttatgcg cgctcaatat ccggtttggt
     7921 ttggtttgct ccgtcgcggc tccgtcggag cgatctgtgt cggcttggct acgacggtgc
     7981 tggccggctg ctcgaatcct gaaagtggcg gcaaattaaa ctgcccgccg ggaatgcgca
     8041 cggtctggca cgatccgcta aacggcgtcg gtgaatgtga aatcgtgacg ccgctgtccg
     8101 acggtcatac gccgcgcagt gggctggaac agccgtttcc ggcgatgaac cgtccaagcg
     8161 gggcgctgac aacaccggaa cgaattgaac tcgggcggct gctctatttc gatccggtcc
     8221 tgtcgggcga caacaccgtg tcgtgcgcgc actgtcacca tccggcgtat ggctttaccg
     8281 acaatcggcc cgtttcgacg ggaatcggcg gcaagcaggg cgggcgcagc gcaccgacgg
     8341 tgtggaacgc gatgtggaac agcgagcagt tttgggacgg acgggtcaaa acgctcgaag
     8401 accaagcgaa ggggccgatc caaaacccga tcgagatgaa ccaagatccg gtcaagctcg
     8461 tcggcgagct gcaagcgatt cccgaatacc gtcagcgttt cgacgaagcg ttccgcggca
     8521 gcaatggatc ggcggtgacg ctcgacaaca tcgcccttgc gattgccgcg tttgagcgga
     8581 cgattgcgtc ggtcagctcg ccgtttgatc gctatgccgc cggggatcgc tcggcgatga
     8641 acgaatcggc ccagcggggt ctgaatctgt tccgctcggt gagcacgcgc tgttttgagt
     8701 gtcacgagat cccgaccttt gcgatgaagg aatacaaagt actcggggtg ccgtcgctgg
     8761 ccaatccgac catggtggac gacgatcgcg gtcgggcggc gattatgccg ggtgcagcct
     8821 atgagcgtgc gttccgcgtt ccgacgctgc gcaacatcac caagaccgcg ccgtatatgc
     8881 acaacggatc gttcaagacg ctcgacgaag tgctcgattt ttacgccaaa ggcggcgggc
     8941 ttgggcgtgg ctacaagctc gacaacgtcg acgacaagat ccgcaagttt gatctaagcg
     9001 ctgacgaaaa agccgatctg atcgcgttta tggaagcgct gaccgacgaa tccctggttc
     9061 ccgaagtgcc gaaaacggtg ccctcggggc tgcccgtcgc caaaggtccg ctgtaacgac
     9121 gtatgatcca gccgcggctg cccaaatagg tcgcggtttt ccgacgaaag gtatgagctg
     9181 catgtcgaaa gccacgaccc tcttttgtct cgcctcgctc tctggttttc ttttgtcggg
     9241 ctgcgccgag cggcctcttt acgactgtta catgggggcg cagaacgccg acgagcgacg
     9301 cgactgcatc gccagcggaa aagcggatgc caccgtcgca ccgggccagt cgatcaccca
     9361 agcggtgttc gatatgcccg ccgatgcgac gcgctgggtc atcaaagtca tgcctggcac
     9421 ttatcgcgaa accgtgtctg tcgaccgcag tggcgtgatg ctcgttggtg tgacgtatgc
     9481 cgataaaccc gacgagcgag tcatcatcga cggtggactc gacggtggaa agttccgcaa
     9541 agacgcggtg atcgtcagtg gttcgcgctt tgtcatggac ggcatcacga tgcgcggctt
     9601 ttccggcaac ggcgtcacca ccagcaagag cagcggcgtc acgttgcgca atctcgtcgc
     9661 agacaagacc ggccactatg gcgtttatcc ggtggaatcg gaagacatcc tggtcgaaga
     9721 ctgcgtgacc acgggcatta atgacgcggg catttacgtc ggccagagcc gccgcgccac
     9781 cgtccgtcac aaccaggtct ttggcaacgt cgctggcatc gaaatcgaaa acaccgtcac
     9841 tgcgctcgtc gaagaaaacg atgtccacga caacaccgcc gggatcatgg cgtttgtgct
     9901 tccgcaagca ccgagcaaag aaggccgcga ctgcacgatt cgcaacaaca agtcgtatcg
     9961 caacaacctc aaaaactttg ccgatcccaa cgctttcgtc gccaagcttc cctacggcat
    10021 cggtatcggc gtgatggctg ccgacggtac ggttgtcgaa aaaaacgaaa tcagcgacaa
    10081 cagctcgacg ggcgtggcgc tgatcggttt gcagctgttc ttttctgacc cgaccaagct
    10141 cgacgtcgag ccgaacaccg accgcgcccg tgtgaccaac aacacctatc gcggcaacgg
    10201 caaagctccc gacccggctt acgtgaacgg ggcgccggtc aaactaaatg gcgccgacat
    10261 catgtgggac ggcaccggga ccggcaactg cgtcgacgaa ccgcaaggtg gagagctaaa
    10321 gaccgacgcg gcggcatcag ctctgccgcg ctgcaagtaa gtcgcgttcg cccagttacc
    10381 agctcgcgac ggcgcgatcc cggaaaaaac caccgcgggg accgtctgcg ggcagcgtcg
    10441 ccagccagac cggcgtgtcg gcgccttctt cgacgctgcg atcggcgctc ggtcccccca
    10501 tatcggtctt tacccagccc ggacagcacg cattgatgac cagatcgcgc ggatcgtccg
    10561 ccagctcgcg cgacagcgtt tcgcaaagta cattgagcgc cagcttcgac acccgatagg
    10621 ccgactgtgg ccagccgtgc gctcgatgct gtcccatcgc caccgcctgt tcaaagtctg
    10681 ccaagagcgc gtgcagttcc gcgcgtgaaa ggggtgtgga aaagcgggtg cgcagcgacg
    10741 gacccaatgc gctgcgatcc gccattccgc tcgacagcat cacgactttt ccatgccgcg
    10801 caagcagcgg caaaaatgca aaagtggcac gctctgcgcc ataaaagttc accgccaatg
    10861 tccgtcgcac gatgtccagg ttgaagcctt ccagcgcgat ccccgcattg ttgaccagcg
    10921 cgtcgatctg tccgacttgc gcggaaaccg attcgacggc ggcgaggatc gatgccggat
    10981 cgctgacatc cagcgcgagc gcacgcgctt ggccacctgc tgcgcgaatc gaactggcga
    11041 caacatcacc gtcgtggagc tgccggcagc caacgaaaac cagaaagcct ttgtgggcaa
    11101 gctggcgcgc gatttcggca ccgatgcctc ggtttgcccc agtgacgacg gcgacggacg
    11161 gcgacaggat cgacatgaaa tcgagcgtat cacgtcgccc ggcgccagcg tttgtgcggt
    11221 cgatgtgggg tggcgcttag cgaacgaacg tgaagcggta gtttgcgccg tacttggaat
    11281 cccagctccc atcgtcggcg acgaacgcga gctcaatgtc ccagttgttg gcgctgccga
    11341 agctgtcgcg ggcgtagaaa aacagagcgc gctcagcgtc gggggccggg acgtaatacc
    11401 aggtcgtgcc accaccgggc tttgctggca gcggacagag cctggctggc ggcgacggct
    11461 tccagtcggg gttgttgctg tcggcaatcg agcaaatgta ttcctcctgc gggtcgccga
    11521 gccggaccga ctcttcaaag ctggcgtttt gcatacggaa tgtgccgtcg cggccattga
    11581 gcttcacata cgcggaaatc cggccccact gcgtcgccag cagcgagccg cgatagcgca
    11641 cgacaagctg cggcatcccg tagtgctgat tttgcgcgca gacttcgacg gggccttcgc
    11701 tcttgcaggt ggtttcgatg acgtagcttg gcggatagaa gctggcctgg gcgtcggggg
    11761 cgccaaacag cacgccggat gcaagcagca gcggaatcac gttttgggac cggttcatgg
    11821 gtttcctccg gggcgtggcc ttttttgcct gcacgccgca gcgcgataca atatcgccgc
    11881 ttctgcttgg cgatgatcgg ttttgctttc cgtcgccgat gcgaatcgca accgacctgt
    11941 gaggcttttc ggcgatgaga aagacgatct gggcgctggc tggtttgctg ggcattgcgc
    12001 ctgcgggaat cgcgcgagcc gacttttccc cggaatacga gctgggggtg tttggcggcg
    12061 cgcatctttg gaacccgctc ggtggcgtcg gtcggcccga tgacagtgaa gcaagccaag
    12121 tcgagcacgg cggcctgctg ggggtccggc tgggcttggg cctgcacacc cgctttgcgc
    12181 tcgaagccga gcttgggctt gcgccgtctt ttaccaaggg acttgcgagt gcaaatgacc
    12241 ccacgttcga tgtgtcggcg cgcgtgctcg gcatcgggta tcgcatccag ggtctggttc
    12301 acattctgac cgggcgattt cgtccgtatg cgctcatcgg gattggcggt tttacgacca
    12361 cgtcgtcgag cgctgcactg tttggccaag acaccaccta cagcgcctct gtgggcggtg
    12421 gtctgaaagt ggatctgggc aaaaactggg gactgcgtgt cgacggcaga gcgcttatgc
    12481 acaaaggtct ccccaacgga cctgcgattg cccccgacgg tgaggtttcg ctcgggttgt
    12541 ttggccggtt tggcgacgtg aaacagacgg ccagttcgat cggtgtcgcc gatcgtgaca
    12601 aagatggaat taccgacgga gcggatctgt gtcccgacgt gccggggatt cctgctttgc
    12661 gtggttgtcc gcagccggct ggcgtacttg tccccccttc tgcggtgccg ccggcgaata
    12721 cctccacggt gacgctgccg tcgctgccgc cgnnnnnnnn nnnnnnnnnn nnnnnccccg
    12781 ccgtaacctg gcaccgtaag atgtcgacca tgacgcagcg cttctgtgac aaatccggcg
    12841 agccgctctc gctggttctt tacatgttcg acgagtgtcc gttttgccag cgtgtgcttt
    12901 cggcgatggg ggcactcggg tttgaccttc cgatgcgcaa cattcgcaag gatctttcgg
    12961 cgcgcgatga actgattcgc gtcggtggca gcaagcaagt gccgtgtctg ttcataaacg
    13021 gcaagccgct ctacgaatcg gcagacatca tcgattacct caaagccgag gtcagacggg
    13081 catagcctga tcgattcgtc gctgcgcggc gggaatccac agacttcgtc gccgggtgcg
    13141 cttgggtctt gtcgccgggc gagggaatgc caaaatgggc gcatggcgat gcagaacttg
    13201 cgagacaagc tgctcaaggc gggtctcgtc gacaaaaagc agaaagttca ggtggatacc
    13261 caggatcggc gcgaaaaaaa gcagcgcgga gccgatcaac tggcggcgga agaagccgaa
    13321 aagcagcgcc tgttcgccga aaaacaagcc gctgaggcgg aagctcgacg acaagccgat
    13381 ttggccaagg tcgccgagcg ggcgagccat gaagccgcca accgcgttcg caacatctgt
    13441 gaccgctggg ccgtccgatt gaccaagccg ggacagcgac gcttttattt tgtccagcga
    13501 tcaggtcgcg tcggctatct gtggctaccg gaagtcatct gcgatcagct ctgcctcggg
    13561 gcactcgcga ttgtggaacg cttgccgacg gaagaaacga ccgactgggc cagccgtccg
    13621 cgcatcaaag gtcgcaagcc gcgcagcctg ctgcaaaagc tcgtcagtca ggtgaaaaag
    13681 ggcgaaatgc agttcgtgag tgaagaaacc cacgttcttt tgccgcccga gcccgcggac
    13741 cgaattttgg cgattgatgc gtcggcggtg cgcttttggg cgcgcagtgg caagccactt
    13801 ggctatgtgg cagaagcggg ggattcggcg ggcgcggcat aagggtcgcg gcggtacaga
    13861 agccacagcg cgagagccat gccgaaaagc gaaatgatct ggctcgtcga caaaaagccc
    13921 atcagcacgc cgcgctcatc gtctcgccag acctcgacga caacgcgcaa aatcgcatac
    13981 agggcaagac acgctccggc gatgaagcct tcttggcgct tgcgcgggcg cacccaaaaa
    14041 taggtcaccg caaacagcag cagacagccg agcgattcgt aaagctgcgt cggatgcacc
    14101 ggcaacatct gctgtccggc catgatctgg tgcgcttcgt actgcgctcg ccacggggag
    14161 ctgccgatgg gaaagcgcac gccgaagagc gagtttgtga ttttgccgta gcagcagccg
    14221 ttcagataac agccgagccg accaaaaaac agcccgagca ggatcgccgg tgccgcgaga
    14281 tcggccacgc gcaggaagcg aatgttggcc cgacgggtgt aatacaccgc ataagccgtc
    14341 gccaggatca gtccgccgta atagaccagc ccgcctcgcc acagcttggc ccaggcaaag
    14401 cagtcgcgcg gcggatagca caggtgccgc gtcgtgtcgc agaggtagtc ataaccacac
    14461 tcacgcgcgg tgttgcagtg cttcaccagc gcgtcaatgg ccggcacttt caaaggatcg
    14521 atgcagaggt ttacgtagtc ttgcaggtgt ccgtcgacaa acacatgcaa aatgcgcgat
    14581 ccgatgaccc cgaaaatggt catgtacagg ttgatgtcga tgatgtcgtc ggggtcgagg
    14641 tcgagcttgc gtgattcacg gatggtcagg accgccgcaa aaaacagtcc cagcgtcaga
    14701 aaggtgaagt aggccgggaa tgtgacatcc ccaagcagcg gcaggacgaa cttccaaaga
    14761 agcggcttca cggctggtta cgccgatgcg ggcttttcag ccgtcggacc ggcgggggcg
    14821 gtctcaccac ttgcgggctg agactcttcc ggcggcagcg gatcgcgtgg acggtcaatc
    14881 aggatcattc cgacgccgat gaccagcgct gcgtcggcga cattgaacgt cggccactca
    14941 taagtggtgg acgacgtcgt gatcttccac aagatgaagt cggtcacccg gctgtacatc
    15001 gcgcgatcga ccatgttgcc tatcgcgcct ccggccagca gccccagctt gaccccgagc
    15061 cactttgcat tcggtggcag ccggtacagc cacaggccaa tcacacccaa gcaaacgatg
    15121 ccaattccga acaggaagta gcgcgcgccc gcctggttgc gaaacatgcc aaacgccgag
    15181 ccggggtttt ccgaataacg gaagtcccaa tagcctttga tgacggtttt cggcgctgaa
    15241 atcgcgttgg ggtagttggc gggctttagg gtctggcggg cccaaatctt ggtgctttgg
    15301 tccagtccga ggctcaccag gaacagcagg aggaaggcga tgtattttga cttcatcgtg
    15361 ttagccgcga gttatatcac ttgagctttc tcttcggcaa cgccgggcgc tcggcaaata
    15421 ttcctgccgg ccctggttat gccgctgtat gcgtggccac ttggtcgtga tgcgcggcga
    15481 tggcttgggc gcgttcggcg actttttggc gcagctcggc gggttcttgc acttcgacgc
    15541 cggcaccaaa gcccaaaatc caactgacca gccagtcgga cagtggattt tcgatggtga
    15601 cttgcaggct gccgtcgcgt gcgacttgga cgcgggccgg atcgaagcgg gttcgcactt
    15661 cgtaggcgag cgttttgtca aagcgcagct cgatccgtgg gtgcgcagct tcgggctgga
    15721 aaaggtggtc gcgacggaat ttttccagat cgaagtcgcc agggacgaca aacgagggca
    15781 cgtcgctgtg aatcacgaca ttcgacagcc gctcgacttt gaacaggcgc ggttcgttgt
    15841 gacggtgaca gcggccaacc agataccagg acccgcggtg gttgaggagc gcatagggat
    15901 cgatccgtcg ctgctcggtc tggccacgcg atgcggagtg atatttcgcc gtcagtggtc
    15961 tccgcgccgt aatcgcccga tggagatccg acagcagtga cagcttgtga tggtcttcgg
    16021 tttcgacgac ggactcgcgt tcaagcgcga gcagttccgg cgatgcctca ccgagcgcgg
    16081 cgcgaatttt ggccagcagc cttgcgaccg cgtcttcata cgggcgaacg cccgatccgg
    16141 ccagcgcccg cagcccgagg gtgagcgaaa acgcttcagc cgcggtcaga cggagcggac
    16201 gggtcagccc ttgcggcagt tcggcgtata cacagtcgtc ttcgtcgtcg atggtgagca
    16261 gcaaaaattc atctggtgcc ccggcgggcg gacccacctg cgacagcaga tcaatgt
//
LOCUS       DAORGN010000107        16270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1463_length_16270_cov_4.974961, whole genome shotgun sequence.
ACCESSION   DAORGN010000107 DAORGN010000000
VERSION     DAORGN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16270
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1463_length_16270_cov_4.974961"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKL17_12680"
     CDS             <1..1639
                     /locus_tag="PKL17_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNK45636.1"
                     /translation="EHPLLRARALRALGQMGRLGDATGHAHHPDPLVRQAVVPWLPVE
                     ELIRLLGEDPDPLIPREVLRQAYRRRAELSEPDRKRLIAAAAKSPDPWLRSHVVSLLS
                     PEDDLPLLLSLARDAQRSVRQAVADKLDGSAGQKEQIHALLKTGQLDEEQQAAAYAEC
                     LKIALLEPNQSEALADLAGELRRPDLPTLALPIRAAVAALRGDLSAPIETPTVYPTRL
                     PSLKQPSNHPPAPRRMLGKTQMEVSPLALSGRCELPPRAFARALDAGVNLFFWEPGYE
                     ALSRLLIAQRKRLHIIAGSYEGDRDSIVADVDRALRRLRTDCLDVFLLFWVRSPERLS
                     DEVLATFEQLKAAGKIRAAGFSTHDRSLAELAVRTGGWDVLMTRHSAAHPGAERSLFP
                     LCAEREIGLLTFSATSYGRLLEPLPGVLAQPVRFSAADCYRYSLAQPGVSSVISAPQF
                     PSELDENLAVLTSQQIAPETRLALLQHGEAIHAVSRRFNALVRKGHESPLHAPNEQAN
                     LSTRLAELLAEHPAPPSEPLAMVTARRQPKNRPERRS"
     gene            1905..3365
                     /locus_tag="PKL17_12685"
     CDS             1905..3365
                     /locus_tag="PKL17_12685"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HNK45637.1"
                     /translation="MSLSNLLQKLIPLCESLDDNWEQVVDLLDAHKELAEYEVARFYV
                     SRYTTRTIANLVKSDDPRLRQKAALLIRLSSTARPAGRHLRILLKDPNARVRSAAHRA
                     VRALRLDDVALPDTRFRKPRSNRYQALGGWNPSGWAFGLYGNMWTIRQYRKQQRKPKT
                     QLADLGVPKLETRKDVLHWLKIPSLRELNKLLRAGSGPGSPYVSFPIPKASGETRTIT
                     APRMTLRKIQRRILHELLSKLPTHNAAHGFVKKRSVVTNAKPHEGAQLVVKLDLKDFF
                     PTISYHRVRGLLCGYGFAQEVADVLAGLTTHRQVLSDGYVVWPGVLPQGAPTSPVLAN
                     LICRQLDSRLSGLAKRMGARYTRYADDLTFSFAKGPDQKNDLGRFLWWINQICQQEGF
                     AENVAKRKILRPSAQQRITGVVVNSGLTVPRAMRRTFRAILHNCQMHGVASQARGKKD
                     FAAYLLGYASYIRMVQPQLGQRLVAEVKALLQKPAP"
     gene            complement(3369..4955)
                     /locus_tag="PKL17_12690"
     CDS             complement(3369..4955)
                     /locus_tag="PKL17_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNK45638.1"
                     /translation="MTSRAASSSSASPNADDLGQSARRGAVFIPLAKLWFMVSALLLQ
                     LLLPRVLGSSALYGVWTLVSSWLSTLNNVMITATIQAVAYFAARGTQHVEEAKRTALR
                     MNLLIGGGAAALFFLLAPVVANFEHDAELVRYLQVGSLIILGYSFYAVFVGAANGARQ
                     FHKQAGLDITFATLRTGLVLAGAYLLHSALAAFWGWVFAALVILVLSVLLVGLPKKTD
                     GEPIPIKTMLGFGTWLAVYLLGLNALMFMDGWWLKRLYTEALSSLPADEVKRTVDALV
                     GVYGAAQTVARLPYQLIVAVAFIVFPLLSAPAVQADKARTERYVSATLRYSLIAMLGL
                     VAALGVRPQATLRLLFPPEYVTGSAALAILLGAYVCFSLFSIVGTITNSLGRTVHTAL
                     LGLVTLVLSAVAVYVSIGSALAAGEQPLRAAALGMLLGMGGGLLLGLLYLWQMLRATL
                     PVMTLLRCGIAFFAVLLMGRFWPAAGSVGLLGSKVGTLICSALSGAVYLAVLLILREL
                     SVKELLLLRRERPTSPPVEG"
     gene            complement(4992..6656)
                     /locus_tag="PKL17_12695"
     CDS             complement(4992..6656)
                     /locus_tag="PKL17_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNK45639.1"
                     /translation="MTTTNKTSQWFCRRGVCQAGLLGPLLLLSHPVYADRISKVVVYP
                     DRAQVTRTAQVACGDKVQTRFASLPPAADVQSVRAAIDVAGAQVLGLRTETEVLKSPF
                     AKQVEQIDEEIRQIDAELRQLGEKRGRERSVEAVATRYEDVAVAMLGRELVDPPGNPQ
                     VVAQLPKAWSLAVDAPLKLRLEHAAGVAQVEKTTRELQKKREELVSRRQRLSAASGQS
                     QLHAEVLVSCPGGADRKAQVELSYFVGGAGFSMEHEARLSDKGTVELKSYATLHQHTG
                     EDWRDAQVVVSTAIPRQNATPPDLVPLRITATERQPPKRLLVSRVEEQVHREASGGLV
                     ATTTASTGKPGVALQEQGLSVQFVAPGTADVTGDGTPVRIRIAQAELRGKVGYRTIPK
                     LQPYVFRVADLVNTAGYPLLAGPIDVFRKGQFSARYGLSRVAAGERFELSFGLVDRVK
                     VKRSVLEEISRDRGVFGTSRRVRYGYRYEIESYLPSGEEIELQEHIPVSELDDVKVAL
                     DAKATPGYTLNAPDGIVTYKLRLSPGEKRAVELHYYVDAPASLLGE"
     gene            complement(6669..8738)
                     /locus_tag="PKL17_12700"
     CDS             complement(6669..8738)
                     /locus_tag="PKL17_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNK45640.1"
                     /translation="MSRRLTLLPLIWLRIGLVPQVSVAATDVVAPVTAVTVYSDRARV
                     LRTTRVALRGGTGTRVELPLLPAGVDASSIRVELDASRSPDAEVSRVEIGFVQPSEVK
                     LPSHEAEKLLAELESLDDQLAQLAAEQSAYAAQLRLIGRLLPSANGLGQPTGTLQVPP
                     PRLNPLGWGAVMGWTAQLYDRVQKKQHELSEKQRQIHLSRRVLVEKGQQLGGLLRRGG
                     YRVTATVTGSGSADLLLGYLIGGAHWRPSYDIQFVPSQNRVELKLAGLVKQQTGEDWT
                     DAQLTLSTAVPATATQLPKLLTWKLGERERFIPTPRPQPEYVPPPPRIPPRPPVVDDD
                     PQARVEQRLLAKLGMPQRGPVVDGRFANVRDAEKSRFDQNAQEESPALERDADGIPDA
                     VDVVPMQAAPMATPPPPPPPPPGRPEAVMAAPVMQSSELSMQVRVDGKTKAKRYPPTS
                     ATGSTVREAEVIGFGLAPPPGYQPVSSQLASESGGYDLVFPALAPETVKSGGGERRVA
                     LLSRSFPVEVSRKVMPALAPEAFLIAEIKNPSPEPLPAGAAQLFVGADPAGVAQLKLM
                     APGEVVPLPLGLDRAIRPARNVQVTTVEKGVFSKDEISEYTVTTELTNPYKVPIQLRL
                     YDQVPLVADKNVEIQLVRSEPPATFDEKTGNLEWRPQLPPGSKLSTKFVYTLKRPKGY
                     RLGQSQY"
     gene            complement(8766..10178)
                     /locus_tag="PKL17_12705"
     CDS             complement(8766..10178)
                     /locus_tag="PKL17_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNK45641.1"
                     /translation="MSSSQLERKLGAVDAAALLVGSMIGSGIFIAPSLMAKNIAAPGL
                     YLGLWVLAGLFTLLGAFSYAELAAMMPEAGGQYVYLRRAFGPLPGFLYGWTLFLVIQS
                     GFIAAVAIAFAKYLGIFFPSVGEEKMWLSLYQGSHGPVGLSSAQGVALLVIAALTYIN
                     TRGVVAGAAVQNLFTGLKLLGLWGLLALILILGKGSPDHFAPLWSTDVPQLPGAVQAG
                     FLAALGVAASKALFCYDAWNTVTFAAAEVRDPARNLPKALIFGTLVTTLTYVVAAAGY
                     LYLFPLTQMAQIPENRIAAEAARSLLGSVGGKLIAAAILVSTFGCINGMILGGARVLY
                     AMAEDGLFFRRAGTLNRNRAPGWALLLLGGWSAVLTMSGKYDDLLTYTTFASLLFNAA
                     TAACVLVLRRKLPDVDRPYRALGYPASTVAFIVVAIGFVVYIVQGDPRSSLYGLGLVL
                     LGVPAYFVLGRKQAAASDVA"
     gene            10264..11028
                     /locus_tag="PKL17_12710"
     CDS             10264..11028
                     /locus_tag="PKL17_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45642.1"
                     /translation="MGDPVPSREPVLRLSKITKVYTRGDLAVPALRGIDLTIDQGEFV
                     AVMGPSGSGKSTLMNLIGCLDRPTSGSYFLQGRDVAKLDRDALAGVRLRTLGFVFQGF
                     NLLSRTTAIENVELPLLYAGLKSDERAKRAKEALSRVGLGDRGGHTPNQLSGGQQQRV
                     AIARGLVTKPMLLLADEPTGNLDSRTSLEVMALLQELNAEGLTVLLVTHEPDIAAFAG
                     RLIVVKDGRIRSDEKRPPQSAKQAVAEWRDSEEAFT"
     gene            11025..12248
                     /locus_tag="PKL17_12715"
     CDS             11025..12248
                     /locus_tag="PKL17_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45643.1"
                     /translation="MMFVMALRLALRSLLRNRMRSALTALGVIIGVAALLVTVVLGEG
                     TRKMVAAQIATMGSAILMIVPGASAQGGFMGGTGSGRPLQRDDVEAILQARSVRYAVP
                     VDRTIAQVISANLNWQTPLLGTTTEYAALRDWPVGRGRFFTSAENDTAAKVCVLGQTV
                     AQNLFGDTDPVGQQIRVKQMLCEIIGVLTPKGQSAMGSDQDDILIMPSLTLRTRVLNQ
                     NRQFVSTIMVAATSEDDLAAAESEVVSILRQRHKLTDEQENDFTIRNLAELVQTQQKI
                     VETQTGMLRNVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGARERDILLQFLVE
                     AVVLSVLGGLLGIGLGVLMATLFSRFGGGMPVDFSPFWMVLGFGVSVAIGVLFGFYPA
                     RKAARLDPIDALRYE"
     gene            complement(12267..13598)
                     /locus_tag="PKL17_12720"
     CDS             complement(12267..13598)
                     /locus_tag="PKL17_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNK45644.1"
                     /translation="MAHYRIDTVLRVRTRHRPGQLARVATVIAQEGALIGEISTLHMA
                     EGLSTREITVETESEEHTERLVSALSRTDGVEIISVRDRVFDCHRGGKLHSKSRVELQ
                     HLSDLRYIYTPGVARVSRAIAEKPERAFDYTGLGNSVGIFTNGTRVLGLGDIGPVASL
                     PVMEGKAVLYDRFAGISATPILVDTKDVEEFVQTVVRVSRSFGGIHLEDIRIPECFRI
                     EEELKRRLRKPVMHDDQHGTAVVTLAAIINVCRLTGIELRRSKMGQVGLGAAGSAIAK
                     LALAYGVGEVMVTDRNPAAVQPLTDAGAHAMDLDSLLQRADIVVCTTGKIGLIKPEQV
                     RRGQVIFALSNPYPEIDPDVARDAGAAYAADGASINNALAFPGIFRGALAVRAGRIDP
                     QMYIAAAEAIAASAGSVEVVPSPLSTTVHDAVTDAVSSCATRLGIANTAEL"
     gene            13797..14699
                     /locus_tag="PKL17_12725"
     CDS             13797..14699
                     /locus_tag="PKL17_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45645.1"
                     /translation="MNTPDAGLTLCQGDGCIGTACAKDADCTEGNAGKAATCWIATIL
                     NNPKLLPTPGGYCSRECDTDADCGTGKCYTLPNASKRYCMARCDSASTCRKPGYACAF
                     EGSSDAICFPNANFDCNPIANQGYCDYGADKYLGGCMRVAYEDDHGGVCHLQCKLGTK
                     TCPADARAGTPAPSQQCLYVDAGVDSHGNVSNPRDAFKGNICFRQTAAPKTPGSPCSN
                     LGECQDGYECDLYAARTADRVCRALCVQGDGMQLDLPGLLVPAGAMIASNVCANAGEI
                     CADVLLAGSRIGSRDRTAGLCVPP"
     assembly_gap    14752..14851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14942..15556
                     /locus_tag="PKL17_12730"
     CDS             14942..15556
                     /locus_tag="PKL17_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45646.1"
                     /translation="MRHVTLAALFAIATTQIASAQPMPGRQRQAELRHDRMEVHRDKA
                     QIADDRRDLIRFQNTLNAFDAAVARRDAMGVRAALTSFVQQGRAEVNEQTREVGQANR
                     EAMRSGGEQMRDQNRKDARDARDDRRDAMKESAALVEERNLLAELERSTAAEYAWGPQ
                     VPILVRSREIMVRFVELARAELARSRTELKEDRRELREDRRGIR"
     gene            complement(15560..>16270)
                     /locus_tag="PKL17_12735"
     CDS             complement(15560..>16270)
                     /locus_tag="PKL17_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45647.1"
                     /translation="KTMARTVYAQGVLTEQNKSEKLQLPEGKTGPFDVTVSGTLKNEG
                     SQNSSAVYDLQVAGEGASETLSGEFSVRTNQMRVRRGTSTWTEQTNQVEHRLARSLRG
                     NSLTVTVEHIDDLLADGLHVSVHPQSYDPKWFFLLGIFVVLGMMYAESKIGDAKMKPH
                     LIMASASTLVFAFHFHEHATASRVVKPALDSLILAVLTGGIGGTMIGAVVRRASGRDK
                     VKAKDDEEKKPKAKSDEE"
BASE COUNT         3221 a         5156 c         4817 g         2976 t
ORIGIN      
        1 tgaacatccg ctgttgcgcg cccgtgcgct gcgcgccctt ggccagatgg gacgactggg
       61 cgatgcgaca ggacacgccc atcatcccga tccgctggtg cgccaagcag tggtgccgtg
      121 gctgccagtc gaagagctga tccgtctgct tggcgaagac cccgatccgc tgatcccgcg
      181 tgaagtgttg cgtcaagcgt accgtcgacg ggctgagctg tcggagccag accgaaagcg
      241 cctgattgcc gctgctgcaa agagtccaga tccgtggctg cgatcgcatg tggtgtcgct
      301 gttgtcgccg gaagacgatc tgccgctgct gctttcgctg gcgcgcgatg cgcagaggtc
      361 tgtgcggcaa gcggtggcgg acaagctgga tggctcagcg gggcaaaagg aacagatcca
      421 tgcgctgctc aaaacgggtc agctcgacga agaacagcaa gccgcagcct atgccgagtg
      481 tctgaaaatc gcgctccttg aacccaacca aagcgaagca ctcgccgatc tggcgggcga
      541 gctgcgccgt ccggatctgc ctaccttggc gctgccgatt cgcgcggcgg ttgcggccct
      601 gcgaggggac ttgtccgcgc cgatcgaaac gccgaccgtg tatccgaccc gattgccgtc
      661 gctcaagcag ccgtcgaatc atccaccagc gccgcgccgc atgctgggca aaacgcagat
      721 ggaagtgtca ccgctggcgc tgtccggtcg ctgtgagctg ccgccgcggg cgtttgcgcg
      781 agcgctcgat gcgggggtga acttgttttt ttgggagccc ggatatgaag cactttccag
      841 gcttcttatt gcgcagcgga agcggctcca catcattgcc ggaagctatg aaggcgaccg
      901 cgattcgatc gtcgcggatg tcgaccgggc actgcgacgg ttgcgcaccg actgtctgga
      961 tgtgtttctg ttgttttggg tgcgctcgcc tgagcggctc agtgacgaag tgctggcgac
     1021 gtttgaacag ctcaaagcgg cgggaaagat tcgcgcggcg ggcttttcga cccacgatcg
     1081 cagcctggca gagctggcgg tccgcacggg cggctgggat gtgctgatga cccggcacag
     1141 cgcggcgcat ccaggggctg agcgttcgct gtttccgctt tgtgccgaac gtgaaatcgg
     1201 actgctgacg ttttcggcga catcgtatgg gcgactgctc gagccgctgc caggggtttt
     1261 ggcgcagcct gtgcgttttt cggcggcgga ttgttatcgg tactcgctgg cgcagccggg
     1321 ggtctcgtcg gtgatctcgg caccgcagtt tccgtcggaa ctggatgaaa acttggcggt
     1381 gctgaccagc cagcagatcg cgccggaaac ccggttggcc ctgcttcagc atggcgaagc
     1441 gattcacgca gtcagtcggc gattcaatgc gctggtgcgc aaaggccatg aaagtccgct
     1501 gcacgcgccc aacgaacaag ccaacctgtc gacgcgactg gccgagctgc tggccgaaca
     1561 ccctgccccg ccgagcgagc ctctggcgat ggtgactgcg cgccgccaac cgaaaaaccg
     1621 cccggagcgc cgctcatgat ccgacatcag gcgctttccc gtcgaaaata cgcagcgctt
     1681 cccatttttg cggcgccctg cgaacatagc gtccagagcg ttccttcgct tgccacgcag
     1741 ttagcttcct ccgcgatgtt acgtcgtggt tgctttcgac ctgcactgga caatgcgctg
     1801 gtccgagctg ccgcttgcgg cttttctcag ctcgggcacc gctgccacgc aagtagctgc
     1861 gaacgaagga acgctttttc tttttcccgc agggaaccac gccgatgagc ctgtccaacc
     1921 tgctgcaaaa gttgattccg ctgtgtgaat cgctcgacga caactgggaa caggtcgtgg
     1981 acttgctcga tgcgcacaaa gagctggcgg aatatgaagt cgcgcgtttt tacgtcagtc
     2041 gctacacgac acggacgatc gcgaatttgg tcaaaagcga cgatccgcgc ctgcgtcaaa
     2101 aagcggcctt gctgattcga ctcagcagca cggcgcgacc ggcgggccgg cacctgcgaa
     2161 tcctgctaaa agatcccaat gcgcgggtgc gatcggcggc gcatcgggcg gtgcgagcgc
     2221 tgcggctcga cgatgtggcg ctgccagata cccgctttcg caagccgcgc agcaatcgct
     2281 atcaagcgct cggaggatgg aatccgagcg gctgggcgtt tgggctgtac ggcaacatgt
     2341 ggacgattcg gcagtatcgg aaacagcagc gcaagcccaa gacgcagctc gccgatctgg
     2401 gcgtgccgaa actggaaacg cgcaaagatg tgctccactg gctgaaaatc ccgtcgctgc
     2461 gcgagctaaa caagctgctg cgagccggca gtggaccggg cagtccgtat gtgtcgtttc
     2521 cgattccgaa agcaagtggt gaaacacgga cgatcacagc gccgcgtatg accctgcgca
     2581 aaatccagcg gcgcatcttg catgaactgt tgagcaaact gccgacgcac aacgccgcgc
     2641 acggctttgt caaaaagcgg tcggtggtga cgaacgccaa gccgcatgaa ggcgcgcagc
     2701 ttgtcgtcaa gctcgacctg aaggactttt ttccgacgat ttcgtatcac cgcgtgcgcg
     2761 gacttttgtg tggatacggt ttcgcacagg aagtggccga tgtgctggcg gggcttacga
     2821 cgcatcggca ggtgctcagc gacggatatg tggtgtggcc gggtgtgctg ccgcagggcg
     2881 caccgacttc gccggtcctg gccaacttga tctgtcgaca gctcgattcg cggctctcgg
     2941 ggctcgcgaa gagaatgggc gctcgctaca cgcgctacgc cgacgatctg acgttttcgt
     3001 tcgccaaggg gccggatcag aaaaacgacc tggggcgttt tttgtggtgg ataaaccaaa
     3061 tctgccagca ggaaggcttt gccgaaaacg tcgccaagcg aaaaatcctg cgtccgtcgg
     3121 cccaacagcg cataactggc gtcgtcgtca acagcggcct gacagttccg cgggcgatgc
     3181 gacggacgtt ccgcgccatc ctccacaact gccagatgca cggggttgca agccaagcgc
     3241 gtggcaaaaa agactttgcc gcctatctgc tcgggtatgc ctcgtacatt cggatggtgc
     3301 agccgcagct tgggcagcga cttgtggccg aagtcaaagc gctgctccaa aagcccgcgc
     3361 cgtagcgtct acccttcgac gggcggactt gtgggacgct cacgccgcag aagcagcagt
     3421 tctttgaccg acagctcgcg caggatcaaa agaaccgcca aatacaccgc gcccgacagc
     3481 gccgaacaga tcagcgtccc gactttgctg ccaagcagac cgacgctgcc cgctgctggc
     3541 cagaaacgac ccatcagcag cacggcaaaa aacgcaatcc cgcagcgcaa aagcgtcatc
     3601 accggcagcg tcgcacgcag catctgccac agatacaaaa gcccgagcaa aagcccgccg
     3661 cccattccca gcagcatgcc gagcgccgcc gcccgcagcg gctgttcgcc tgccgcaagc
     3721 gccgaaccaa tcgacacata gaccgccacc gccgacagca cgagcgtcac aagcccaagc
     3781 agcgccgtat gcaccgtccg tccgagcgaa ttggtgatcg tgccgacgat gctaaacagc
     3841 gaaaaacaga cgtaggcgcc aagcaggatc gcgagcgcag cgctccccgt cacatattcc
     3901 ggtggaaaca gcagtcgcag cgtcgcctgt ggccgaaccc caagcgccgc caccaaaccc
     3961 agcatcgcaa tcagcgagta acgcagcgtc gccgatacat accgctcggt ccgcgccttg
     4021 tccgcttgca ccgccggagc cgacagaagt ggaaacacaa tgaacgccac cgcgacgatc
     4081 agctgatacg gcagccgcgc caccgtctgc gccgcgccat atacaccgac gagcgcatcg
     4141 accgttcgct tcacctcatc ggcgggcagt gacgacagtg cttcggtgta cagccgcttg
     4201 agccaccagc cgtccatgaa catcagcgca tttaacccga gcagatacac cgccagccac
     4261 gttccaaagc cgagcatcgt ctttatcggt atcggctctc catcggtctt tttgggcaga
     4321 cccaccagca gcaccgacag caccaagatc acaagcgccg caaagaccca gccccaaaac
     4381 gccgccagcg cactgtgcaa aagatacgcc ccggccagca ccagtccggt ccgcagcgtc
     4441 gcgaacgtga tatcgagccc cgcctgtttg tgaaactgtc gcgccccgtt ggccgcaccg
     4501 acgaacaccg cgtaaaagct gtagcccaag atgatgagcg atccgacttg cagatagcgc
     4561 accagctcgg cgtcatgttc aaagttggcg acgacggggg ccagcaaaaa gaacagcgcc
     4621 gctgcaccgc cgccaatcag cagattcatg cgcagcgccg tccgcttggc ttcttcgacg
     4681 tgctgggttc cgcgcgcggc aaagtaggcg accgcttgaa tcgtcgccgt gatcatgacg
     4741 ttgttcagcg tcgacagcca cgatgacacc agcgtccaga cgccgtacag cgccgaactg
     4801 ccgagcaccc gcggcagaag cagctggagc aagagcgccg acaccatgaa ccacagcttg
     4861 gcgagcggaa taaacacagc tccgcgacgg gccgattgac ccaagtcatc ggcattgggc
     4921 gaagcagatg aggaagacgc agcgcgagaa gtcatggatt gtttggcctg ccagcgcagg
     4981 aaatgggcag tttactcccc caagaggctt gcgggggcat cgacgtagta atgcagttcg
     5041 actgcgcgct tttcaccggg gctgagccgc agcttgtagg tgacaattcc gtcgggcgca
     5101 ttgagcgtat agcccggcgt cgctttggca tcgagcgcca cttttacatc gtccagctcg
     5161 gaaaccggga tgtgttcttg cagctcgatc tcttcgccgc tgggcagata gctttcgatt
     5221 tcgtagcggt agccgtatcg gacgcgccgg ctggtgccaa acaccccgcg atcccggctg
     5281 atttcttcga gcacgctgcg ctttactttg acgcggtcga cgaggccaaa gctaagctca
     5341 aagcgctcgc ccgccgcgac ccgcgaaagg ccgtaacgtg ccgaaaactg gcctttgcga
     5401 aacacatcaa tcggcccggc cagcagcgga tatcctgccg tgttgaccag atccgcgacg
     5461 cgaaacacgt aaggctgaag cttgggaatc gtgcgatagc cgactttgcc gcgcagctcc
     5521 gcctgggcaa tccgaatccg gaccggcgtg ccgtcacccg tgacatcggc ggttcccggc
     5581 gcgacaaact gcaccgacag accttgttcc tgtagcgcga cacccggttt cccagtcgat
     5641 gcggtcgtcg tcgccacaag tccccccgat gcttcccgat gcacttgctc ttccacccgg
     5701 ctgacgagca gacgcttggg cggctgccgt tccgtcgcgg tgattcgcag cggcaccaga
     5761 tccggcgggg tcgcattctg ccgcggaatc gctgtcgaga cgaccacctg cgcatcccgc
     5821 cagtcttccc cggtgtgctg atgcagcgtc gcgtagcttt tcagttccac ggtccctttg
     5881 tcggacagcc gcgcttcgtg ttccatcgaa aaaccggcac caccgacgaa atacgacagc
     5941 tcaacctggg ctttgcggtc cgcgccgcca ggacagctca ccagcacttc agcgtgtagc
     6001 tggctctgac ccgacgccgc cgaaagacgt tgccgacggc tcaccagctc ttcgcgtttc
     6061 ttttgcagct cgcgcgtcgt tttttcgacc tgggcaaccc ccgccgcgtg ttccagccga
     6121 agcttgagcg gcgcgtcgac cgcgagcgac cacgccttgg gcagctgggc caccacctga
     6181 ggatttcccg gcggatcgac cagttcccgt cccagcatgg ccaccgcgac atcttcatag
     6241 cgcgtcgcca ccgcttcgac ggaccgctcg cgcccgcgct tttcgccaag ctgccgcagc
     6301 tctgcgtcga tttggcgaat ctcttcgtcg atctgctcga cttgcttggc aaacggcgat
     6361 ttgagcactt ccgtttccgt ccgcagcccc agcacctgcg cccctgccac atcgatggcc
     6421 gcccgcaccg actgcacgtc cgcagccggc ggcagcgacg caaagcgcgt ctgcactttg
     6481 tccccacagg ccacctgcgc cgtccgcgtc acctgcgccc gatcgggata caccaccact
     6541 ttgctgatcc gatcggcgta caccgggtgc gacaaaagca aaagcggtcc cagcaggccc
     6601 gcctggcaaa cgccccgtcg acaaaaccat tgactcgtct tgttcgtcgt cgtcatggtg
     6661 gacccccgtt agtactgcga ctgcccgagc cgatagccct tgggacgttt tagcgtgtag
     6721 acaaacttcg tcgacagctt gctgcccggc ggaagctgcg gacgccattc gaggttgccg
     6781 gtcttttcgt cgaacgtcgc cggaggctca ctgcgcacaa gctgaatttc gacgtttttg
     6841 tccgccacga gcggcacttg atcgtagaga cgcagctgga tcggcacttt gtacgggttc
     6901 gtaagctccg tcgtcaccgt gtattcgctg atttcatctt tgctaaacac gcctttttcg
     6961 acggtggtga cctggacatt gcgtgcggga cgaatcgccc gatcgagccc gagcggcagc
     7021 ggcaccactt cccccggtgc catcagcttc agctgcgcca cacccgccgg atcagccccg
     7081 acaaatagct gcgccgcacc cgccggcagc ggttcgggac ttgggttttt gatctcggcg
     7141 atcaggaacg cttcgggggc cagcgccggc atcacttttc ggctgacctc gacgggaaaa
     7201 ctgcgagaca gcagcgccac gcggcgctcg cctccacctg acttgacggt ctcgggggcc
     7261 agcgccggaa acaccagatc atagccgccc gattcactgg caagctgcga agagaccggc
     7321 tgataaccag gtggtggagc cagtccaaac ccgatcactt ccgcttcacg aactgtcgat
     7381 cccgttgcag aagtcggtgg atatcgcttg gctttggtct tgccatccac acgcacctgc
     7441 atcgacagct cggacgactg catcaccggc gcagccatga cggcctcggg tcgacctggc
     7501 ggtggcggcg gtgggggtgg cggcgtcgcc atgggagcgg cctgcatcgg aacgacatcc
     7561 acagcgtctg gtattccgtc ggcatcacgc tccagcgctg gcgattcttc ctgggcgttt
     7621 tggtcgaatc gggacttttc cgcatccctc acattggcaa atcgcccatc gacgaccggg
     7681 cctcgctgtg gcattcccag cttcgcaagc aagcgctgct ctacccgcgc ctgtggatcg
     7741 tcgtcgacca caggcggtcg cggcggaatc cgaggcggtg gcggcacgta ttctggttgg
     7801 ggccgcggcg tcggaatgaa gcgctctcgc tcgcccagct tccaggtcag aagcttcggt
     7861 agctgcgtcg ccgtcgccgg aaccgccgtc gacaacgtaa gctgggcatc ggtccagtct
     7921 tcgccggtct gctgtttcac aaggcccgcc agcttcagct cgacgcggtt ttggctgggc
     7981 acaaactgga tgtcatacga cggtcgccaa tgcgcgccgc cgattagata gccaagcaaa
     8041 aggtccgccg acccactgcc tgtcaccgtc gcagtcacgc gataaccgcc gcgccgtaac
     8101 agaccgccca gctgctgacc tttttcgacc aacacgcgcc gcgagaggtg gatctgccgc
     8161 tgtttttccg acagctcgtg ctgttttttc tgcacccgat cgtaaagctg cgccgtccag
     8221 cccatcaccg cgccccagcc gagcggattg agccgcggcg gcggtacttg caaagtcccg
     8281 gtcggttgcc caagaccatt tgccgacggc aaaagtcgtc caatcaagcg aagctgcgcc
     8341 gcataagcgc tctgttctgc cgccagctgc gcaagctggt cgtccagcga ttccaattcc
     8401 gccagcagtt tttccgcttc gtgcgacggg agcttgacct cgctgggctg cacaaaacca
     8461 atctcgacgc gcgacacttc ggcgtcgggg ctgcggctcg catccagttc gacacgaatg
     8521 ctgctggcat ccacgccggc aggcaagagc ggcagttcca cgcgcgtccc cgtcccccca
     8581 cgcagcgcca cccgcgtcgt tcgcagcacc cgtgcgcggt cgctatacac cgtcaccgcc
     8641 gtgacgggcg ctaccacgtc cgtagccgcc actgaaacct gcggcacaag cccgattctc
     8701 agccagatca aaggcagcaa agtcaaccta cgcgacatca gcagcacctc gcggatttgg
     8761 cacgtttacg cgacatcaga cgcagcggct tgcttgcgtc cgagcacaaa gtacgccggc
     8821 acaccgagca gcaccaggcc caggccgtac agcgacgagc gaggatcccc ctggacgatg
     8881 taaacgacga aaccaatcgc taccacgata aacgcgacgg tcgaagctgg atagccaagt
     8941 gcgcgataag ggcgatcgac gtcgggcagc ttgcggcgca gcaccagcac gcaggctgcc
     9001 gtcgcggcat tgaacagaag cgacgcaaac gtcgtgtacg tcagaagatc gtcgtatttg
     9061 cccgacatcg tcagcaccgc cgaccagccg cccagcaaaa gcagcgccca gcccggtgca
     9121 cggttgcgat tgagcgtccc ggctcgacgg aaaaacagac catcttcggc catcgcatac
     9181 agcacccgcg cgccgcccaa gatcattccg ttaatgcagc cgaacgtcga aaccaaaatc
     9241 gccgcggcaa tcagctttcc accgacgctg ccgagtagac tgcgcgccgc ctctgccgca
     9301 atccgatttt ccggaatctg cgccatttgg gtgagcggaa acaggtacag ataaccggct
     9361 gccgccacaa cataggtcag cgtcgtcaca agggttccaa aaatcagtgc tttgggaagg
     9421 tttcgcgccg ggtcacgcac ttcggcggca gcaaatgtga ccgtgttcca ggcgtcgtaa
     9481 caaaacagcg ctttcgaggc tgccacacca agcgcggcca aaaaacccgc ttggactgcg
     9541 ccgggaagct gcggcacgtc ggtgctccac agcggcgcaa agtggtcggg actgcctttg
     9601 cccagaatca aaatcagcgc caaaagtccc cacagaccga ggagcttcag cccggtaaac
     9661 aggttctgca ccgctgcgcc agcgacgacg cctcttgtgt ttatataagt cagcgccgca
     9721 atcaccaaca gcgcgacgcc ttgggctgac gaaagaccga cggggccgtg gcttccttga
     9781 tacagcgaaa gccacatttt ttcctccccg accgacggaa agaaaattcc caggtacttc
     9841 gcaaaagcaa tcgccaccgc cgcgataaaa ccggactgga tcaccaaaaa cagcgtccag
     9901 ccgtacaaaa accccggcag cggtccaaat gcccgtcgca gatagacata ttggccaccg
     9961 gcttccggca tcatcgccgc cagttccgca taactaaatg cgcccagcag cgtaaacagc
    10021 cccgcgagca cccacagccc gaggtacagc cctggtgccg cgatgttttt ggccatcagc
    10081 gacggagcaa taaaaatccc cgagccaatc atactgccaa caaggagcgc cgccgcgtcg
    10141 acggccccca gcttgcgttc gagctgtgac gaagacatat cgctcgtggg taacatgtgg
    10201 gcatcgtcgg ctcaattttt tcgccgtcgc acttctctgt tcgtttgggt aaccttccag
    10261 cttatgggcg acccggttcc cagtagagaa ccagtcctgc gactgtcgaa aatcacgaaa
    10321 gtctatacgc gcggcgacct cgcggttccg gcgctgcgcg gcatcgatct gaccatcgat
    10381 caaggcgagt ttgtcgccgt catgggccca agcggttcgg gcaagtcgac gctgatgaac
    10441 ttgattggtt gcctggatag accgacttcg ggatcgtatt ttttgcaagg tcgcgacgtc
    10501 gccaagctcg atcgcgatgc gctggccggt gtcaggctgc ggacgctcgg ctttgtcttt
    10561 cagggcttca atcttttgtc acggacgacg gcgatcgaaa acgtcgagct gccgctgctg
    10621 tatgccgggt tgaagtccga cgagcgggcg aagcgagcca aagaagcgct gtcgagggtg
    10681 gggcttggcg atcgtggcgg acataccccg aatcagcttt ccggcgggca acagcagcgc
    10741 gtcgcgattg cgcgtggttt ggtgaccaag ccgatgctgc tgctcgcgga cgaaccaacg
    10801 ggaaatctcg actctcgcac gtcgctggaa gtgatggcgc ttctgcaaga gctaaacgcc
    10861 gagggtctta cggtgctgct cgtcacccac gaacccgaca ttgccgcgtt tgccggtcga
    10921 ttgattgtcg tcaaagacgg acggattcgc tccgacgaaa aacgcccgcc ccagtccgcc
    10981 aaacaagcgg tcgccgagtg gcgcgacagt gaggaggcat tcacatgatg ttcgtcatgg
    11041 cgctccgctt ggcgctgcga agtctgctgc gcaaccgcat gcgatcggcg ctgacggcac
    11101 ttggtgtcat cattggcgtc gcggcgctgc tagtgacggt ggtgctcggc gaagggaccc
    11161 gcaaaatggt cgcggcccag attgcgacga tgggatcggc gatcctgatg atcgtgccag
    11221 gcgcgtcggc gcaaggtggt tttatgggcg gcaccggcag cggtcgaccg cttcagcgcg
    11281 acgacgtcga ggcgatcttg caagcgcgat cggtccgtta cgccgttccc gtcgatcgca
    11341 ccatcgccca ggttatcagc gccaatctca actggcagac gccgctgctc ggaacgacga
    11401 ccgaatatgc ggcgctgcgc gactggccgg tcggtcgcgg gcgatttttt acgagcgctg
    11461 aaaatgacac cgcggccaaa gtctgcgtgc tcggtcagac ggtggcacag aacctgtttg
    11521 gtgacaccga tccggttgga cagcagattc gcgtaaagca aatgctgtgc gaaatcatcg
    11581 gcgttttgac ccccaaaggt cagtcggcga tgggtagtga tcaggacgat attctgatca
    11641 tgccgtcgct gacgctgcgc acgcgggtgc taaaccaaaa ccggcaattc gtcagcacga
    11701 tcatggttgc agcgacaagc gaagacgact tggcggcagc ggaaagcgaa gtggtgtcga
    11761 ttctgcggca gcggcacaag ctgaccgatg aacaggaaaa cgacttcacc attcgcaact
    11821 tggcagagct tgtgcagacg caacaaaaga tcgtcgaaac ccagaccggc atgctgcgca
    11881 acgtcgccgc ggtatcactt ctcgtcggag ggatcgggat catgaacatc atgctggtgt
    11941 ccgtcaccga acgaacccgc gaaattggca tccgcttggc gattggggcg cgcgagcgcg
    12001 acatcttgct gcagtttctc gtcgaagccg tcgtgctgtc ggtgctagga ggcctgctcg
    12061 gaattgggct tggcgtcttg atggcgacgc tgttttcacg tttcggggga ggaatgccgg
    12121 tcgatttttc gccgttctgg atggtgttgg gcttcggcgt gtcggtggcg attggcgtgc
    12181 tgtttggctt ttatccggcg cgcaaggcgg cgcggctcga tccgattgac gccctgcgct
    12241 acgaataagt ccgcgggacg atttttttat agctcggcgg tgttggcgat tcccagtcgg
    12301 gtcgcgcagc tagacaccgc gtcggtgact gcgtcgtgga ccgtcgtgga cagcggtgat
    12361 ggcaccactt cgacgctgcc cgccgatgca gcaatcgctt ccgcagcggc gatgtacatc
    12421 tgcggatcga tccgcccggc ccgaaccgcc agcgcaccac gaaagatgcc cggaaaggcc
    12481 agcgcgttgt tgatcgaagc gccgtcggca gcataggcag cgccggcatc acgcgcgacg
    12541 tccggatcaa tttcgggata gggattggac agcgcgaaaa tgacctgacc gcgacggact
    12601 tgctcgggtt tgataagccc aattttgccg gtcgtacaga cgacaatgtc ggcacgctga
    12661 agcagtgaat ccagatccat cgcgtgcgct ccagcgtcgg tcagcggctg caccgcggct
    12721 ggattgcgat ccgtgaccat gacttcgcca acgccgtaag cgagcgccag cttggcaatc
    12781 gcggaacctg ccgcgcccag accgacctgc cccattttcg atcgccgcag ctcgatcccg
    12841 gtcagccgac agacgttgat gatcgccgcc agcgtcacca ccgcggtgcc gtgctggtcg
    12901 tcgtgcatca ccggcttgcg cagtcgacgc ttcagctctt cctcgatgcg gaaacactcg
    12961 gggatgcgga tgtcttcaag gtgaatgccg ccaaagctgc gcgacacccg aacgacggtc
    13021 tggacaaact cttcgacatc tttggtgtcg acgagaatcg gcgttgcaga aatcccggcg
    13081 aagcggtcat acagaaccgc tttgccttcc atgaccggca agctggcgac ggggccaatg
    13141 tcaccaagcc cgagcacgcg cgtaccattt gtgaaaatgc cgacggagtt gccgagtccg
    13201 gtgtagtcaa acgctcgctc gggtttttca gcgatggccc gagaaacacg cgccacgccc
    13261 ggcgtataga tgtagcgaag gtcggatagg tgctgtagct cgacgcggct tttcgagtgg
    13321 agcttgccac cgcggtgaca gtcaaacacc cgatcgcgga cgctgatgat ttcgacgccg
    13381 tcggtgcgag agagagccga aaccagtcgt tcggtgtgct cttcgctttc ggtctcgacg
    13441 gtaatttcac gggtcgacag accttctgcc atatgcagcg tcgaaatttc cccgatgagt
    13501 gcgccctcct gcgcaatcac agtggccaca cgcgcaagct ggccgggacg atgacgggta
    13561 cgaacacgga ggacggtatc aattcggtaa tgagccatgg cagcccttaa tctaccgcac
    13621 ctcctgctac gtcctctacc cctcacatct gtcgtgcgtc ggctgtcgat tggcgggtaa
    13681 aatgacgcgc acgggaggat gacctaatga ctacttggat taacagggga ctaggcgctg
    13741 tcgttggaat gctattgctg ggccaggttg gctgcggcac gaccaatacg ccgaccatga
    13801 acacccccga cgctggactt acgttgtgcc aaggcgacgg ctgcatcggc acagcgtgcg
    13861 caaaagacgc tgactgcacc gaaggcaacg ccggcaaagc ggcgacttgc tggattgcca
    13921 ccattttgaa caacccaaag cttctgccga cgccgggcgg ttattgcagc cgtgaatgtg
    13981 acaccgacgc cgactgcgga accggcaagt gttacaccct cccgaatgcg agcaaacgat
    14041 actgcatggc tcgctgcgac agcgcttcga cgtgccgcaa gccgggctac gcctgtgcat
    14101 tcgaaggaag cagcgacgca atctgtttcc cgaatgccaa cttcgactgc aaccccatcg
    14161 caaaccaagg ctactgcgac tacggcgccg acaaatacct cggcggctgt atgcgtgtcg
    14221 cctatgaaga cgaccatggc ggcgtctgtc acctgcagtg caagctggga accaagacct
    14281 gccccgccga tgcccgcgca ggaacgcctg ccccatcgca gcagtgtctt tacgtcgatg
    14341 cgggcgtcga cagccacggc aatgtgtcaa acccgcgcga tgcgttcaaa ggcaacatct
    14401 gtttccgtca gaccgccgcc cccaagaccc ccggatcgcc gtgcagcaac ctgggggaat
    14461 gccaggacgg ctatgagtgc gatctctacg cggcgcgcac cgccgatcgg gtctgtcgtg
    14521 cgctctgtgt tcagggtgac ggcatgcagc tcgacttgcc cggtctgctc gtccccgcgg
    14581 gtgccatgat cgcaagcaac gtctgcgcca atgccgggga aatctgcgcc gacgtgctgt
    14641 tggccggctc gcgcattgga tcacgcgatc gcaccgccgg tctgtgcgtc cccccgtaaa
    14701 ctcgcagtga atcaagctgg ggaatcgcac gtcgacacag aacagaacag annnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaaactcgc agtgaatcaa gctggggaat
    14881 cgcacgtcga cacagaacag aacagacaac cagtttcacc tttcaaacgg agacagacat
    14941 catgcgccat gtgaccctcg ccgccctgtt tgccatcgcc accacgcaga ttgcttctgc
    15001 ccagccgatg ccaggtcgtc aacgccaagc cgagcttcgc cacgatcgga tggaagtcca
    15061 ccgtgacaaa gcgcagattg ccgacgaccg ccgcgacctg atccgtttcc aaaacacgct
    15121 caacgccttt gacgctgccg tggctcgtcg cgatgcgatg ggtgttcggg ccgcgctgac
    15181 cagcttcgtg cagcagggtc gcgccgaagt caacgagcag acgcgcgaag tcggacaggc
    15241 caaccgcgaa gcgatgcgat cgggcggcga gcagatgcgg gatcagaacc gcaaagacgc
    15301 ccgcgatgcg cgcgatgacc gtcgggatgc aatgaaggaa agtgcggcgc ttgtcgagga
    15361 acgcaacctg ctcgccgagc tggagcgatc gactgccgcc gagtatgcgt ggggaccgca
    15421 ggtgccgatt ctggttcgct cacgcgagat catggttcgc tttgttgagc tggcccgagc
    15481 cgagctggcc cgttcgcgca ccgaactgaa ggaagaccgc cgcgagctgc gagaagatcg
    15541 ccgcggcatt cgctagtcgc tattcttcgt ccgacttggc cttgggcttc ttttcttcgt
    15601 cgtccttggc cttgaccttg tcgcggcccg acgcccgtcg caccaccgca ccaatcatcg
    15661 ttccgccgat tccacccgtc agcaccgcca gaatcagtga atcgagcgca ggctttacca
    15721 cccgcgacgc tgtggcgtgt tcatggaagt ggaatgcgaa caccagcgtc gaagccgaag
    15781 ccatgatcag gtgcggtttc atcttggcat cgccgatctt gctttcggcg tacatcatcc
    15841 ccagcaccac gaaaatgccg agcaggaaga accacttcgg atcgtagctt tgcggatgga
    15901 ccgacacatg cagtccatcg gccaacagat cgtcgatgtg ttcgaccgtc accgtcagtg
    15961 aatttccccg cagactgcgc gcgagtcggt gttcgacttg gtttgtctgt tcggtccacg
    16021 tcgatgtgcc acgacgaacc cgcatttggt ttgtgcgcac cgaaaactcg ccgctcaggg
    16081 tctcgcttgc gccttcaccg gccacttgca ggtcgtacac cgccgagctg ttctgcgagc
    16141 cttcgttttt cagcgtaccg ctcaccgtga catcgaacgg accggttttt ccctcgggaa
    16201 gctgcagttt ttcggacttg ttctgttcgg tcagcacgcc ttgggcatag accgtccgcg
    16261 ccatcgtctt
//
LOCUS       DAORGN010000108        16179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1475_length_16179_cov_6.060903, whole genome shotgun sequence.
ACCESSION   DAORGN010000108 DAORGN010000000
VERSION     DAORGN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1475_length_16179_cov_6.060903"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKL17_12740"
     CDS             <1..823
                     /locus_tag="PKL17_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNK45648.1"
                     /translation="VHRLYEMARKGQLAFPAINVNDSVTKSKFDNLYGCRESLADGVK
                     RATGVMFAGKVAVVAGYGDVGKGCAQSLRGYGARVIVTEIDPIIALQAAMEGYQVTTM
                     EEAAPLGDIFVTATGCMNVIRGEHMLAMKDQAILCNIGHFDCEIDMAWLCAQPGVQEV
                     NIKPQVDQFVFPDGKRLTILARGRLVNLGCASGHPSFVMSTSFSNQTIAQIELWCNPG
                     KYPVGVYMLPKKLDEKVAALHLAKLGVKLTKLTAEQAHYLGIPQDGPYKPDHYRY"
     gene            826..2337
                     /locus_tag="PKL17_12745"
     CDS             826..2337
                     /locus_tag="PKL17_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNK45649.1"
                     /translation="MSSALSRRRFLQHAGSLLVLAGSPPRDGARARASVATPYQNGLW
                     LAGDHHIHTKYSSDGNYEILAQVKAAAQNGLGFCVITDHGGPAHEKVVTSRAYAELVE
                     ARRACPEIIIFAGLEWNIPAAEHGSIIIPPTEDEARLIALFESRFDSKSRKTPPLGEA
                     DAIAGVSFLQSLPHKPLLFANHPARRGLDSPHELRAWRDAGPDVMRGFEGAPGHAAAP
                     LVGERRGHYSDKPSAGSFADYPLDSYRTHGGYDYYTAQVGGLWDALLSEGRPFYITCN
                     SDSHLYLGDVQKFDRRNYKTTGVVTATGESRSYGPSDERGDTDYAPGIYTETKVFADR
                     RDPLSVLSGMRSGNMYTCLGGMIDALRCYVHDGVHTAPMGGYLHIPSPGATVELVVEI
                     EVASRRNLGGIMPSLDHVDLIVGDIVPGANTGDSFRNPTARVLRRIEAKACRRSGNRF
                     SFRHVFTDCRRSFYLRLRGTNTPVVVPQIDPPDIDPWTDLWFYGNPIFVMVKR"
     gene            complement(2375..3010)
                     /locus_tag="PKL17_12750"
     CDS             complement(2375..3010)
                     /locus_tag="PKL17_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45650.1"
                     /translation="MKTSNLINNLVLVDAILTMGLGLGLGLTAPALVIGGLVPLLGAL
                     LSLFLLATGLGPMFKGAGAVENNDPNLIAAEKADAQGRMIYLVVSIAAILTLALGLIA
                     HKTELALIAGGLVPIGGLVLALVAGAGSVFGGKTEAESLQNFTSFGPVEEQTFVVFER
                     TSLETEKRASRIAVLSVGSVGLLFALIVLVFGGKGEHVNPAAAPAPAAEKK"
     gene            complement(3101..3784)
                     /locus_tag="PKL17_12755"
     CDS             complement(3101..3784)
                     /locus_tag="PKL17_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45651.1"
                     /translation="MHSTASEHSSQPLLQPSPSRISELSQAACDYVQRATGLELDSSE
                     DSLAFADHYLRTVQSGKKLRPEVADLVASALGVYLGELLLSKFGGRWLAVPTEPTGDD
                     KDGTPVLDSIDDPLGFRVQLHAAPLICDPVLWARQALLVGSDDDTQSDEGGLVVPPSL
                     QDKLHAAMARLPPVSEEYYFSFTGRFETVCYLVELLASLLSENADDTNENDGGEAGST
                     DGANIANPH"
     gene            3972..4703
                     /locus_tag="PKL17_12760"
     CDS             3972..4703
                     /locus_tag="PKL17_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNK45652.1"
                     /translation="MRIGIFSDTHANLEALEAVLECFRVARVDKLVCLGDTVGYGADP
                     NGCCDLVRKHAAFCILGNHDAAVAGRMDYSYYYDAARQALDLHSGILTDENMLWLQNL
                     PYERREGHICFCHGSPINLEEFEYIFSTEQAARCLPFWDQLAEVTFIGHSHLCKAFAL
                     SKSEVHEVVAQKFEVRPGYRYIISVGSVGQPRDYDNRASYCIYDDQTRIFEFRRVAYD
                     IDSAAQKIFEANLEPNFGNRLYIGV"
     gene            complement(4754..5110)
                     /locus_tag="PKL17_12765"
     CDS             complement(4754..5110)
                     /locus_tag="PKL17_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNK45653.1"
                     /translation="MRTVSKPWGHELIWAHTDRYVGKILHIKRGESLSLQYHQKKDET
                     IMVLAGKLRFEHYSEGEEPRHTDLSPREPFHITPGLRHRMIALEDTDVVEVSTPELDD
                     VVRIEDRYGRKGTSAP"
     gene            complement(5152..7377)
                     /locus_tag="PKL17_12770"
     CDS             complement(5152..7377)
                     /locus_tag="PKL17_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45654.1"
                     /translation="MSGSSMSRFRVLWAIGLVWLLFGLQKSAFADDEGRLSGRVFVRG
                     SIVPLPEAAIEVRVADTSRQTHSDPQGRFDLQVPIGDAQIKVSHPDFETLRTSAKITP
                     HTSIEPRLQRRPAYQQPFSATVRSPQNKAEGQSISLRGDELRTLPGSLGDPFRAVGLL
                     PGVATPVPLLPVYVVRGASPGMNGFYLDGMRVPQLFHLLVGGGVVHARLVEQMDFYPG
                     AYDASFGRYAGGIIDAKTRAARSDGYHGELELRLFDLSALVELPLPKDVRITLSGHYG
                     YPGPIIHAIDSRVDVGYWDYQLRLDWKGLTVQALGSYDSVSISDPSLSGGSGNPPVDN
                     TFRTTFHRLQVRYRHQIGRHAVEVGAVGGIDEMTIFQGNGVRKMALNLRAQAQLVWPK
                     IKLRLGTDTEFSRFSAQNFGDVDVRARPDDIGDLGGSRDGIVASAYAVLSVEPLPWLT
                     LTGSGRIDLYHAGAVTLLGIDPRFQVRARLTDFLLIHAGIGYYQQPPSFPVALPGIDT
                     YALSLGLQHATHAAVGQELTLPYSLSLTLAGFYQKYQNSNDVVIDFTPQLCTSPPPES
                     LSGYAAVITRQVDGASYGMELMLRRHHGRFTGWVAYTLSRAERAFTCGLRPADYDQPH
                     VLNTVAQVRLPWNLMIGGRFFYSTGRPVTILKPPDGSATVRNADRLPDTIQLDLRIDR
                     EWIFKSFALDVFLEAVNVTYSQAVFGLTYPREGSITRYDQPQLNGFRWVLPSIGVRGR
                     F"
     gene            7400..8212
                     /locus_tag="PKL17_12775"
     CDS             7400..8212
                     /locus_tag="PKL17_12775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK45655.1"
                     /translation="MSSSEGSSSWGPFFGRGEQREPMIFDEMAVFAALLRRRKVRVTF
                     GLIGLYVVLFALSELWGGSDSVLTLVAMGAEVPQLIKEGQYFRLLSATALHGGLLHIL
                     LNSYVLYSFGPFLEKLFGWQRFTVLYIVSGLCGTLLGTLLGELVGFQVSVGASGSLFG
                     LIGAAAVLGFRDFGLPADLVTQLRKNALINLGLNVLVSLRPHVDWVAHLGGLVAGVIL
                     LLTFVKPKLTDEDTPADKVFAGLAILLVVALIGSAAIAIWVGQPWQVASLPS"
     gene            8237..9748
                     /locus_tag="PKL17_12780"
     CDS             8237..9748
                     /locus_tag="PKL17_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45656.1"
                     /translation="MRRLSLLRKLILAMLLPSLIAFVVFGLLARQAAARAVEAELGRR
                     LQQAAQLIARQISDESVALLQPGDEDSRTHRNLRRRLSELRDTASLSRVYVFASDHSL
                     RVDTSGSPIGAKLYQLDGNRSELGKIFAAASAQATRSVSSVLFRGHDGQIYKSGFAAI
                     ESDSGAVHFAVGVDGSAALYADLVGLQRTLLFVGVLASLLLTLLALWLGRQVSQPLVR
                     LSEGAQAVGRGVLDRPVPSELLRGDDEVAVLSQTLETMREALLARDERMQMMLAGIAH
                     EVRNPLGGMELFAGLLREELTPNEHVPLDPLQARSYVDRILRELLHLQRVVADFLLYA
                     RRPSPALQTVDLATLLGEVCDSVQAIGEKVAVKTVLPDATVVQVKADPQQIRRAVENL
                     ARNAVQACEGRAEALVTLSVSLVSKTRCAIQVRDNGPGIPDDVLPKIWTPFFTTRAQG
                     TGLGLPFVREIIHDHHGTIRVETSAAGTVFTLELEAVPCETDSPRPPLGPLGG"
     gene            9785..10723
                     /gene="thyX"
                     /locus_tag="PKL17_12785"
     CDS             9785..10723
                     /gene="thyX"
                     /locus_tag="PKL17_12785"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010300017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNK45657.1"
                     /translation="MSLSAAQVEAVREAQSQTAATRRPTVAALESLLHEPFSVLDHGF
                     VRVVDYMGDDAAIVQAARVSYGRGTTRLSSDRGLIRYLVRHRHTTPLEMCEIKLHVKL
                     PIFVARQWIRHRTANVNEYSARYSILDREFYLPHPDQLAEQSRSNRQGREQLLSQEQS
                     ARVLRLLQTDAERCYDHYEELLNEDDDGNPRREGDPQLARELARMNLTLNYYTQWYWK
                     VDLHNLLHFLSLRMDSHAQYEIRVYADVIGSIVEKWVPQAWEAFRDYRLEAVSLSRME
                     LAAIKSMLRGEKPDLAALGLSAREERELLQKLDLPG"
     gene            complement(10731..12356)
                     /locus_tag="PKL17_12790"
     CDS             complement(10731..12356)
                     /locus_tag="PKL17_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNK45658.1"
                     /translation="MSEQCKYDEQVLDYLYGELAEAERAEFANHLATCASCRLEIESL
                     SGVRKKASSLPRPELTSDAAAKMRAQLMEAAIAATKDQKQSLGGGKLIAFPTGRVRRF
                     MTHPATALVTVAAAALFLVVFKGKDQPAPVLDSPLPVAKIEAPAKLEPAAAPSPTGAT
                     PPATQVPVIAEIDGKPSVGKTASPAPSLNAGDVPVGGIAAKSETISPSMPTGGVFDRK
                     LADATARTRVVAGTAATTTAAPASKKPMVWGPTPSSTPQEYAKSDRAATERGDTNDGR
                     RFAQPPPPPAAEPQAPQVVAQAAPQAAPAPAMKAAEEEAPGNIVEQSKARQHKRTVDE
                     LERGELAQAPSGAMDNEGYARRPASTGSYGKATSKVSSLNKDSDDDLSNVGTAGPGAV
                     ADKQAEKNNQATPIAQVYEQIRAGHCTEARDLLAKLERSSPSMTGLADANTAWQRDCG
                     AKQMLQNNIQQQNGMPVREQQLQQNYQNLQQRNLALPPAPSQAAAPAKMERSFEPMEQ
                     RRAMAKKAAPLPAPPMKAKAPAKAKAAAADAAY"
     gene            complement(12353..13003)
                     /locus_tag="PKL17_12795"
     CDS             complement(12353..13003)
                     /locus_tag="PKL17_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNK45659.1"
                     /translation="MHVSGIPYQAQTSSVILRTWVRDEANEMLMLRFRDGDPRAFELL
                     VERHQRPIFQFVMRSVGTKAVAEELTQETFLRVIKQKDHYEQRARFTTWLYTLARNLC
                     IDHSRRMQHRRAKSLDAVDEEGHSLLERTADKGLGVDRQAISDELQARMRKAIDSLPD
                     DQREVFLLREEADLSFKEIADIVGISENTVKSRMRYALTKLRSLLEEYEDMARALS"
     gene            13083..14378
                     /locus_tag="PKL17_12800"
     CDS             13083..14378
                     /locus_tag="PKL17_12800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45660.1"
                     /translation="MLDRVGSCRIESELASGGMAVLYRAVQETLDRPVAVKALKTAVQ
                     EDEAFAQRFEREARTLAQLQHENLIHVYEFLREQGALFIVMELVDGIDLYDLLEKVPR
                     LPPSVVACVGLQIARALSYLHTMGVLHRDLKPANVLISRAGIVKLGDFGIADDPRHSG
                     DLTATGATLGTPSYMSPEQILGERLDWRSDQFSLGIVLYQMLAGRKPFAEDAQHSLPE
                     KIRTQAPSSLQKLCPDAPPALVRIIDRCLRKKRAERYRTPEQLLVALEGFVGETVAES
                     GQAHIVRFLVERGLLPPGELQGLPSPKPQRARRLTIPQVYRAPLAHLLLLGLLLLSVT
                     LWRGLFSATFPSPLVPAWEPDAKTAQLFLTADPWAEVYVDGQLVETTPFATPLRLRAG
                     VRYLELRGPYFRPVRQRIDLKPGERRDLHVQMPLLPRGS"
     gene            14378..14848
                     /locus_tag="PKL17_12805"
     CDS             14378..14848
                     /locus_tag="PKL17_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45661.1"
                     /translation="MRAASWVWLSLCLSILPLGFAQAAFPTKNPVVTRKNVAMPRPRA
                     VASVPREESRPAREDRGRIETRLVAARSEYADGRYAASVLLLLRVLAETQPSLDQQIQ
                     VFDLLAACYVALGQIELAVGSFGEVLHRRPDFALDPVKTSPKVRAALEQARSRL"
     gene            14978..>16179
                     /locus_tag="PKL17_12810"
     CDS             14978..>16179
                     /locus_tag="PKL17_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45662.1"
                     /translation="MTLTLRALLCFSLLGVSFSTARADGPTDYPGYDEGDPNQVVDNG
                     DGQVYQPPPDGVCFDDENQSYDCSKDEDYQSYSNVDDGYDPAAYEDFRDALAPYGQWV
                     QSAQYGSVWVPSPESVGADFTPYYSGGRWNYTDYGWTWVSEYNWGWAPFHYGRWITLP
                     GYGWTWVPGRIWGPAWVHWRTGGGYVGWAPLPPRGMVIARPVVGSRWHYWNFVASHEI
                     MAPRLLRIAPPLLPNIFVRTAITNDFRSIGSTRVIIGPPLHHFGGARIVPAPLGSIQI
                     SLPRSHIVVRPGVPLVSRTYYSPYQRPGWGSTTVSAPRPGFPPVYRRPVYPAPRPGYP
                     APYPRPGYPAGQQPPVYQPPPRPGYPHGPSYPQGPGQHQPWPQGPGQNLPPGQPPVHQ
                     RPGWENRPG"
BASE COUNT         2907 a         4839 c         5100 g         3333 t
ORIGIN      
        1 cgtgcaccgg ctgtatgaaa tggccaggaa gggccagctg gcgttcccgg cgatcaacgt
       61 caacgactcg gtgaccaaga gcaagttcga caacctgtac ggctgccgtg agtcgctcgc
      121 cgacggtgtc aagcgcgcca ctggcgtgat gttcgctggc aaggtcgctg tcgtcgctgg
      181 ttacggagac gtcggcaagg gctgcgccca gtcgctgcgc ggctacggag cccgcgtcat
      241 cgtgaccgaa atcgacccga tcattgcgct gcaagccgcg atggaaggct accaagtcac
      301 gacgatggaa gaggccgcac cgctcggcga catcttcgtc accgcgacgg gctgcatgaa
      361 cgtcatccgt ggcgagcaca tgctggcgat gaaggaccaa gcgatcctct gcaacatcgg
      421 ccacttcgac tgcgaaatcg acatggcgtg gctgtgcgcg cagccgggcg tacaagaagt
      481 caacatcaag ccgcaggtcg atcagtttgt gttccccgac ggcaagcgct tgaccatttt
      541 ggcgcgtgga cggcttgtga acctgggctg cgcgtcgggt cacccgtcgt tcgtgatgtc
      601 gaccagcttt tcgaaccaga cgattgcgca gatcgagctg tggtgtaacc ccggcaaata
      661 ccccgtcggt gtctacatgc tgccgaagaa gctcgacgaa aaagtcgcgg cgcttcacct
      721 tgcgaagctg ggcgttaagc tcaccaagct gacggcggag caggcccatt acctcgggat
      781 tccgcaggac gggccgtaca agccggatca ctaccgctac tagtcttgtc ttctgcgctc
      841 tcgcgacggc ggtttcttca gcatgcgggc agcttgctcg tgttggctgg atcgccgccg
      901 cgggacggag cgcgcgcccg tgccagcgtc gccacaccgt atcaaaacgg gctgtggctc
      961 gcgggcgatc atcacatcca caccaagtac agctccgacg gcaactacga aatcttggcg
     1021 caggtgaaag ccgcggcgca aaacggtctc ggcttctgcg tcattaccga tcacggcggc
     1081 ccggcgcacg aaaaagtcgt cacctcacgc gcttacgcag agcttgtcga agcccgtcgc
     1141 gcctgtccgg aaatcatcat ctttgccggg ctagagtgga acattccggc ggcagagcac
     1201 ggcagcatca tcatcccacc gacggaagat gaagcccgcc tgatcgcact ttttgaatcg
     1261 cgctttgaca gcaaaagccg caaaacgccc ccgcttggtg aagccgatgc catcgccggc
     1321 gtgagctttt tgcagtcgct tccacacaag ccactgctct ttgccaatca tccggctcga
     1381 cgggggctcg acagtccgca cgagctgcgg gcttggcgcg atgccggtcc cgacgtgatg
     1441 cggggtttcg agggcgcgcc cggacacgct gccgcgccgc ttgtcggtga aagacgcggg
     1501 cactactccg acaagccgag cgctggatcg tttgccgatt atcctctcga ttcgtatcgg
     1561 acccacggcg gctacgacta ttacacggcg caggttggcg ggctgtggga cgcgctcttg
     1621 tccgagggac gaccgtttta catcacctgc aactccgatt cgcacttgta cttgggcgac
     1681 gtgcaaaagt tcgaccggcg aaactacaag acgaccggcg ttgtcaccgc caccggcgag
     1741 tcgcgcagct acggtccgtc cgacgagcga ggcgacaccg actacgcgcc ggggatctac
     1801 actgaaacca aggtctttgc cgatcgacgc gacccccttt cggtcctatc cggcatgcgc
     1861 agcggcaaca tgtacacctg cctcggcggc atgatcgatg cgctgcgctg ttatgtccac
     1921 gacggcgttc atacggcccc gatgggcggc tatctgcaca tcccttcgcc gggcgccacc
     1981 gtcgaactgg tcgtcgaaat agaagtggca tcgcgtcgca acctcggcgg catcatgccc
     2041 agcctcgatc acgtcgatct catcgtcggc gacatcgtgc ctggcgcgaa caccggcgat
     2101 agctttcgca atccaaccgc acgggtgctt cggcgcatcg aagccaaagc gtgccggcgg
     2161 tccggcaacc gcttcagctt tcgccacgtt ttcaccgact gtcgccgcag cttttatctg
     2221 cgcctgcgag gtacgaacac ccccgttgtc gtgccgcaga tcgatccgcc agacatcgat
     2281 ccgtggaccg atttgtggtt ttacggcaat ccgatttttg tgatggtaaa gcgctgagca
     2341 gcgagggaaa gacgaggcgg ggcgagaagc gggactactt cttttccgct gccggggcgg
     2401 gggccgctgc cgggtttacg tgctcaccct tgccgccgaa gacaaggacg atgagcgcga
     2461 acagaaggcc aaccgaaccg acgctgagca cggcgatccg cgaggcgcgc ttttcggttt
     2521 cgagcgaggt gcgctcaaac acaacgaagg tctgctcttc cactgggcca aacgaggtga
     2581 agttctggag cgactcggct tcggttttgc caccgaacac gctgcccgcg ccagcgacga
     2641 gcgcaagcac caagccgccg atcggaacca gtccgcccgc gatgagcgca agctccgtct
     2701 tgtgggcaat caggccgagc gccagcgtca gaatcgcggc gatcgacacc accaggtaaa
     2761 tcatccggcc ctgcgcatcg gctttttctg ccgcgatcag gttcggatcg ttgttttcga
     2821 cggcaccggc acccttgaac atcgggccca ggcccgtcgc caaaaggaac agcgacagaa
     2881 gtgcgcccag cagtgggacc agaccgccga tgaccagcgc tggcgcagta agaccaagtc
     2941 caagtccaag acccatcgtc aggatggcgt cgacgagaac caggttgttg atgaggttcg
     3001 aggtcttcat atcagcttcc atgcgttgtc cctccggtgg tccggcttaa tgtgcccata
     3061 ctaattgagg cgtccgcgaa tacaaagcgt cgaccgattc ctagtgtgga ttcgcgatgt
     3121 tggcgccgtc cgttgaccct gcttcgccgc catcgttttc gtttgtgtcg tcggcgtttt
     3181 cgcttagcag gctggcgagc agctcgacca gatagcacac cgtctcaaag cggcccgtga
     3241 acgaaaaata gtattcctcg ctgaccggcg gaagccgtgc catcgcggcg tgcagtttgt
     3301 cttgcagcga cggaggcacc acaagtcccc cttcatcgct ttgcgtgtca tcgtcgcttc
     3361 ccacaagcag cgcttgccgg gcccacagca ccggatcgca aatcagcggc gcggcgtgaa
     3421 gctggacgcg aaaacccagc ggatcatcga tcgaatcgag cactggcgtt ccatctttgt
     3481 cgtcgccagt tggctcggtc ggaaccgcca gccagcgtcc tccaaacttg ctgagcaaaa
     3541 gctcgcccag gtacacaccc aaagctgaag cgaccagatc ggcaacttcg gggcgcagct
     3601 ttttaccgct ttgcaccgtc cgcaggtagt gatcggcaaa cgcgagcgag tcctcgcttg
     3661 aatcgagctc cagcccggtc gcccgctgta catagtcaca ggcggcctgg cttagctctg
     3721 aaatgcggct tggcgacggc tgcaaaagcg gctggctgga atgttcagat gcggtgctgt
     3781 gcatcggggc tctcggtcat ctaaagaaaa gaacaagcaa gcgaacgccg gaaccagtct
     3841 ggcgcgcaag gtggggcgga ttgtgcgtgc gattgggcag cagggcaaga cccgcgagcg
     3901 tcggaccggt ggcgagtttg cgctgaccgt tgccgcttgg gcagttttca aagtacagta
     3961 gggcttccgt tatgaggatt ggtattttct ccgataccca cgccaacctt gaggcccttg
     4021 aagccgttct agagtgtttt cgcgtggcgc gcgtggacaa gctggtctgc ctcggagata
     4081 ccgttgggta cggggcggac cccaatggct gctgcgatct ggttcgcaag cacgccgcgt
     4141 tctgcatcct gggcaaccac gatgcggctg tcgctggacg aatggactat tcgtactact
     4201 acgacgcagc gcggcaggct ttggacctgc actcgggcat cttgaccgac gaaaacatgt
     4261 tgtggcttca gaaccttccg tatgagcgtc gcgaaggcca catctgtttt tgccacggct
     4321 cacccatcaa cctcgaagag ttcgagtaca ttttctcgac ggaacaggcg gcgcgctgcc
     4381 tgccgttttg ggatcagctc gccgaggtga cgttcatcgg tcactcgcac ctgtgcaaag
     4441 ccttcgcgct gtcgaaaagc gaagtgcacg aagtcgtcgc gcagaagttc gaagtccgtc
     4501 ccggctaccg ctacatcatt tcggtaggtt ccgtcggaca gccgcgtgac tacgacaacc
     4561 gtgcgagcta ctgcatctac gacgatcaga cgagaatctt cgagtttcgc cgggttgcct
     4621 acgacattga ctcggcggcg cagaagattt ttgaagccaa cctagagccc aacttcggca
     4681 accggctcta catcggcgtc tgatcgctgc gtcgaaagcc cagcgctgtg tggcgggccc
     4741 aggcggtatg cgcttacggt gcgcttgtgc ctttgcgtcc gtagcgatct tcgatccgca
     4801 ccacgtcatc cagttccggc gtcgagactt ccaccacatc ggtgtcttca agcgcaatca
     4861 tgcggtggcg cagtcccggc gtgatatgga acggctcgcg cggcgaaaga tcggtgtgac
     4921 gaggctcctc cccctcggaa tagtgctcaa accggagctt gcccgcgagc accatgatcg
     4981 tctcgtcttt tttctggtgg tattgcagcg acagcgactc gccgcgtttg atgtgaagaa
     5041 tttttccgac gtaacggtcg gtgtgggccc aaatcagctc gtggccccat ggtttgctta
     5101 cggtacgcat gtcgagcggt gtatttcgcg acgcaggccg tcgtcaagtt cctaaaatcg
     5161 accgcgcacg ccgatcgacg gcagcaccca gcgaaagccg ttaagctgcg gctgatcgta
     5221 gcgggtgatg ctgccttcgc gcgggtacgt aagaccgaac accgcctgcg aatacgtgac
     5281 gttgaccgct tccaaaaaca cgtcgagcgc gaacgacttg aagatccact cgcgatcgat
     5341 gcgcaggtcg agctggatcg tgtcgggcag ccgatcggca ttgcggaccg tggcgctgcc
     5401 gtcgggtggt ttcaaaatcg tgaccggcct gccagtcgaa taaaaaaaac gcccaccgat
     5461 catcaggttc cacggcaggc gcacttgtgc gacggtgttg agcacatgcg gttgatcgta
     5521 atcggcggga cgcagaccgc aggtaaacgc ccgctcggcg cggctcagcg tgtacgcgac
     5581 ccagccggtg aatcgtccgt ggtgccgccg cagcatcagc tccatgccat acgacgcacc
     5641 gtcgacttgg cgggtgatca ccgcggcgta acctgacagc gattccggcg gcggcgacgt
     5701 acaaagctgc ggcgtaaagt cgatcacgac atcgtttgag ttttggtatt tctggtaaaa
     5761 gcccgcgagc gtgagcgaca aagagtaggg cagggttagc tcttgtccga cggcggcgtg
     5821 ggtcgcatgc tgaagtccca ggctgagcgc gtaggtatca atgccgggca gcgcgacggg
     5881 aaagctcggc ggctgctggt agtagccgat gccagcgtgg atcagcagaa agtccgtcag
     5941 acgggcccgc acctgaaagc gcggatcgat gcccagcagc gtcaccgctc cggcgtgata
     6001 caggtcgatg cgaccactgc cggtcagggt caaccagggt aacggttcga cggaaagcac
     6061 tgcatacgcc gacgctacga tgccatcgcg agacccgccc agatcgccaa tgtcgtcggg
     6121 acgcgcgcgc acatcgacat cgccaaagtt ctgcgccgaa aaacgggaaa actcggtatc
     6181 ggtcccgagc cgcagcttga tcttgggcca cacaagctgc gcttgcgccc gcagattgag
     6241 cgccattttg cgcacgccgt tgccttgaaa aatcgtcatc tcatcaatgc caccgacggc
     6301 accgacttcg actgcgtgcc gtccgatctg atgccgatag cgaacctgca agcgatgaaa
     6361 cgtcgtgcgg aacgtgttgt cgacgggtgg attgccgctg ccgccagaca gcgacggatc
     6421 gctaatcgac acactgtcgt aagagccgag cgcttgcaca gtcagccctt tccagtcgag
     6481 ccggagctga taatcccagt agccaacgtc gacgcggctg tcgatcgcat ggatgatcgg
     6541 ccccggatag ccatagtgtc ccgacagcgt gatgcgcacg tctttgggca gcggcagctc
     6601 gacaagtgcc gacagatcaa acagtcgcag ctccagctcg ccgtgatagc cgtcgctgcg
     6661 ggcggcgcgg gtcttggcgt cgatgatgcc gccggcgtag cgaccaaacg acgcatcata
     6721 ggcgcccgga taaaaatcca tctgctcgac gagccgcgca tggacaacgc caccaccgac
     6781 gagcaagtga aacagctgcg gtacccgcat cccgtcgaga taaaaaccat tcatgccggg
     6841 gctcgcaccg cgcacgacgt agaccggcag aagcggcacc ggcgtcgcca caccaggcag
     6901 gagcccgacc gcgcgaaatg gatcacccaa actacctggt aaagttcgta gctcatcgcc
     6961 acgcaggctg atcgactggc cttcggcttt gttttgcgga ctgcggacgg tggctgaaaa
     7021 cggctgctga taggccggtc ggcgttgcag ccgcggttcg atggaggtgt gcggcgtgat
     7081 ctttgcgctt gtgcgcagcg tctcgaaatc gggatgactg actttgatct gggcgtcacc
     7141 gatgggaact tgcagatcaa agcggccttg cggatcgctg tgggtttgtc gcgaggtgtc
     7201 tgcgacgcgg acttcgatgg cggcttcggg cagcggcaca atcgatccgc gcacgaagac
     7261 gcgaccgctc aggcgccctt catcgtcggc aaacgcactt ttttgcaggc caaaaagcag
     7321 ccacacaagc ccgatcgccc aaaggacgcg gaaacgggac atggacgaac cggacatagg
     7381 tggggttata tgatggctta tgagcagcag cgaaggttcc tcgtcgtggg gtccgttctt
     7441 tggccgcggt gaacagcggg aaccgatgat tttcgacgaa atggcggtgt ttgcggcgct
     7501 tttgcggcga cgaaaagtgc gggtgacgtt tgggctcatc gggctgtatg tcgtgctgtt
     7561 cgccttgtcg gagctgtggg gcgggtccga cagtgtgctg acgctggtcg cgatgggcgc
     7621 cgaggtgccg cagcttatca aagaaggaca gtattttcgt ctgttgtcag cgacggcact
     7681 gcacggcgga cttttgcaca ttttgttaaa cagctacgtg ctgtattcgt ttgggccgtt
     7741 tctcgaaaag ctattcggct ggcagcgctt cacggtgctg tacatcgttt cggggctgtg
     7801 cgggacgctg cttgggacgc tgctcggcga gcttgtcggt tttcaggtca gcgtcggagc
     7861 atcgggctct ttgttcgggc ttatcggcgc agcggcggtg ctgggctttc gcgattttgg
     7921 cttgccggcg gatttggtga cgcagctgcg caaaaacgcg ctcataaacc tggggctcaa
     7981 tgtgctggtt tcgctgcggc cacacgtcga ctgggttgcg cacctcggag ggctcgtcgc
     8041 gggcgtgatt ctgctgctta cgtttgtaaa accgaagctg accgatgaag acacgccggc
     8101 tgacaaagtc ttcgcggggc ttgcgatctt gctcgtcgtg gcgctgattg gttcggcggc
     8161 gatcgcgatc tgggtcggtc agccgtggca ggtggcatcg ctgccgtcgt aaccgctggt
     8221 gcgttgggca ttttgtatgc gacgtctttc gctgctgcgc aagctgatcc tggcgatgct
     8281 tttgccgtcg ctgatcgctt ttgtggtgtt cggtcttttg gcacggcaag ccgcggcccg
     8341 cgctgtcgaa gctgaactgg gacgacgtct tcagcaggcc gctcagctga ttgcccgtca
     8401 gatatccgac gaaagtgtgg cgctgcttca gcctggcgac gaagacagcc gcacccatcg
     8461 caatttgcgc cgacggcttt cagagttgcg cgataccgcg tcgctttcgc gggtgtacgt
     8521 ttttgcgtcc gatcactcgc tgcgcgtcga cacttctggt tccccgatcg gggccaagct
     8581 gtatcagctc gacggaaatc gcagtgaact ggggaaaatt tttgcggcgg cttctgccca
     8641 ggcgacacgt tcggtttcgt cggtgctgtt tcgcggccac gacggacaga tctacaaatc
     8701 gggttttgcc gcgatcgaaa gcgactcggg ggcggtccat tttgctgtcg gagtcgatgg
     8761 cagcgcagcg ctgtatgccg atctggtcgg attgcagcgg acgctgcttt ttgtcggggt
     8821 gctggcgtcg cttttgctga cgctgctggc gctgtggctg ggacggcagg tcagccagcc
     8881 tttggtccgt ctgagcgaag gagcgcaggc ggtgggccgc ggtgtcctcg atcgcccggt
     8941 gccgagcgag cttttgcgtg gcgatgacga agtcgcggtg ctttcgcaga cgctggaaac
     9001 aatgcgtgag gcgcttttgg cgcgcgatga acggatgcag atgatgctgg ccgggatcgc
     9061 tcacgaagtg cgcaatccgc tcggtgggat ggagctgttt gccgggcttt tgcgggaaga
     9121 actaacccca aatgagcacg ttccgctcga tcccctgcaa gcgcgaagct acgtcgacag
     9181 gatcttgcgc gaactattgc acctgcagcg tgtcgtcgcc gactttttgc tgtatgcacg
     9241 acgaccgtcg ccggcgctgc aaaccgtcga cctggcgacg ctgcttggcg aagtctgcga
     9301 cagcgtccaa gcgatcggtg aaaaagtggc cgtaaagaca gtgcttcccg acgcaacggt
     9361 ggtccaagtg aaggccgatc cgcagcagat tcggcgagcg gtggaaaatc tggcgcgcaa
     9421 tgccgtccag gcctgtgaag ggcgcgcgga ggcattggtg acactttcgg tgtcacttgt
     9481 gtccaagacg cgctgcgcca tccaagtgcg tgacaacggt ccggggattc ctgacgatgt
     9541 gctgcccaaa atctggacgc cgttttttac gacgcgcgcg caggggactg ggctgggcct
     9601 gccgtttgtg cgggaaatca tccacgatca tcacgggacg atccgcgtcg aaacgagcgc
     9661 cgcgggtacc gtgttcaccc ttgagcttga ggccgtcccg tgtgagaccg acagcccgcg
     9721 accaccgctc ggcccgctag gtggttgaca gatcgccgcg cacttcgata cgacgcgggg
     9781 ctccatgtcg ctttctgctg cccaggtcga ggccgtccgc gaggcccaat cccaaaccgc
     9841 cgccacccgt cgtcccaccg tcgctgcgct cgagtcgctg cttcatgaac cgttttccgt
     9901 gctggatcac ggtttcgtgc gcgtcgtcga ctacatgggc gacgatgctg cgatcgtgca
     9961 ggccgcgcgc gtttcgtatg gacgcgggac gacacgactg agcagcgatc gcggtctgat
    10021 tcgttacctg gttcggcacc gtcacacgac gccgctggag atgtgcgaga tcaagctgca
    10081 cgtcaagctg ccgattttcg tcgccagaca gtggattcgg caccgcaccg ccaacgtcaa
    10141 cgaatactcg gcgcgctatt cgatcctcga ccgcgaattt tatctgccgc atcccgatca
    10201 gctcgccgag cagtcgcgat cgaaccggca ggggcgcgaa caacttctgt cgcaagaaca
    10261 gtcggcgcgc gtgctgcggc tgcttcagac cgacgccgag cgctgctacg accactacga
    10321 ggaactgtta aacgaagacg acgacggcaa tcctcggcgc gaaggtgatc cgcaattggc
    10381 gcgcgagctg gcgcggatga acctgacgct gaattactac acgcagtggt actggaaagt
    10441 ggacctccac aacctgctgc attttctgtc gctgcggatg gattcgcatg cccagtacga
    10501 aattcgcgtc tatgccgatg tgatcggcag catcgtcgaa aagtgggtgc cgcaagcgtg
    10561 ggaagcgttc cgtgactatc gactggaagc ggtgtcgctg tcgcggatgg agcttgccgc
    10621 gataaagtcg atgctgcgcg gcgaaaaacc cgatctggcg gcgctcggtc tgtcggcgcg
    10681 cgaagaacgt gagcttttgc aaaagctcga cctgcccggc taaccgtcgg ctaatacgcg
    10741 gcgtcggcag ctgcggcctt ggctttggcc ggggctttgg ccttcatcgg cggggctggc
    10801 aatggggcgg cttttttcgc catcgcccga cgctgttcca tcggttcaaa cgaccgctcc
    10861 attttcgctg gggcagccgc ttggctcggc gctgggggca gggccaggtt gcgctgctga
    10921 aggttctggt agttttgctg aagctgctgt tcgcgcaccg gcatgccgtt ttgctgctgg
    10981 atgttgtttt gcagcatttg tttggcgccg cagtcgcgct gccaggcggt gttggcgtcg
    11041 gcaagtccgg tcatcgacgg tgacgatcgc tccagttttg ccaaaagatc gcgggcttcg
    11101 gtgcagtggc cggcgcgaat ctgctcatag acctgcgcaa tcggcgtcgc ctggttgttt
    11161 ttttcggcct gtttgtctgc cacagcgccg gggccagctg tgccgacatt cgacaaatcg
    11221 tcgtcgctgt ctttgtttag gctgctgact ttgctggtcg cttttccata gcttcccgtc
    11281 gaagccggtc gccgtgcata gccttcattg tccatcgcgc cgctcggtgc ttgggccagc
    11341 tcgccgcgtt ctagttcatc gacggtgcgt ttatgctgac gggccttgct ctgctcgacg
    11401 atgttgcctg gcgcttcttc ttccgcggct ttcatggctg gagccggcgc cgcttgtggt
    11461 gcggcctggg cgacgacttg gggcgcttgc ggttcggcag ccggcggcgg gggcggttgg
    11521 gcaaaccgac gcccatcatt tgtgtcgccg cgttccgtcg ctgcacgatc ggattttgca
    11581 tactcttgcg gggtcgacga aggggttgga ccccacacca tcggcttttt cgaggcaggt
    11641 gcagcggtcg tcgtagctgc ggtcccagcc acgactcggg ttctcgcggt ggcatcggcc
    11701 agcttgcgat caaacacgcc tcccgtcggc atcgacgggg aaatggtttc gctttttgcc
    11761 gcgatccctc cgacgggaac atcgccggcg ttcaagcttg gggctggcga ggcggttttg
    11821 ccgacgctcg gctttccgtc gatttctgca atcaccggaa cttgcgtcgc gggcggcgtt
    11881 gccccagtcg gtgatggcgc cgctgccggt tccaacttgg ccggagcttc gattttggca
    11941 acgggaagcg gcgaatccag caccggggct ggctggtctt tgcctttgaa caccaccaaa
    12001 aacagcgctg ccgccgccac cgtgaccagt gccgtcgctg gatgcgtcat aaaacgccgc
    12061 acacgtcccg tcgggaatgc aatcagcttg ccgccaccga gagactgttt ttgatctttc
    12121 gtcgctgcga ttgccgcttc catgagctgc gctcgcattt tggcggcagc gtcggacgtc
    12181 agctcgggac ggggcaagga cgatgctttt ttgcgcacgc ccgacagcga ttcaatttcc
    12241 agacggcagc ttgcgcaggt ggccaggtga ttcgcaaact cggcacgctc tgcctcggca
    12301 agttcgccgt acaggtagtc gagcacttgt tcgtcgtatt tacactgctc gctcatgaga
    12361 gtgcccgtgc catgtcttca tattcttcca gcagcgacct tagcttcgta agggcatagc
    12421 gcatccggct cttcaccgtg ttttcggaaa tcccgacgat atcggcgatt tccttgaacg
    12481 aaagatccgc ttcctcacga agcaaaaaca cttcccgctg atcgtctgga agcgaatcaa
    12541 tcgctttgcg catgcgcgcc tgaagctcgt cggaaatggc ctgccgatcg acgcccagcc
    12601 ctttgtcggc ggtgcgttcg agcagcgagt gcccttcttc atcgacagcg tcgagactct
    12661 tcgcgcggcg gtgctgcatc cgtcgcgagt gatcaatgca gaggttgcgc gccagcgtat
    12721 aaagccacgt cgtaaaacgc gcccgctgtt cgtagtggtc cttctgcttg atgacgcgca
    12781 aaaaggtctc ttgggtcagc tcttctgcga cggctttggt tccaaccgat cgcatgacga
    12841 actgaaaaat cggcctctga tgtcgttcca ccaaaagctc aaaggcgcgc ggatcgccgt
    12901 cgcgaaagcg gagcatgagc atttcgttgg cctcgtcgcg cacccaagtc ctcaaaatca
    12961 cagaggatgt ctgagcctga tatggtatcc cagagacgtg cacttagaaa cacccttgct
    13021 cccgccagct ttggttgcgg gcttgttctt tgcgaacgcg cagggcacag aagcgatctg
    13081 tcatgctcga tcgggttggc tcctgtcgca ttgagagcga gctggcctcg gggggcatgg
    13141 cggttctgta ccgtgcggtg caggaaacgc tcgatcgccc ggttgcggtc aaagcgctca
    13201 aaaccgcggt gcaggaagac gaagcgtttg cccaaaggtt cgagcgagaa gcgcgcacgc
    13261 tggcgcagct tcagcacgaa aacctgattc acgtttatga gtttctccgc gagcaaggtg
    13321 cgctgttcat cgtcatggag ctggtcgacg ggatcgacct ctatgacctt ttggaaaaag
    13381 tgccgcggct gccgccttcg gtggtggcgt gtgtcgggct gcaaatcgcg cgcgcgctgt
    13441 cgtatttgca cacgatgggt gtgctgcatc gtgacctcaa gccggccaac gtcctcatca
    13501 gccgggcggg gatcgtcaag ctgggcgact ttgggattgc cgatgatccg cggcactctg
    13561 gtgacttgac agcgacggga gcgacgctgg gcacgccaag ctatatgagc cccgagcaga
    13621 ttctgggcga gcggctcgac tggcgatcgg atcagttttc actgggcatt gtgctttacc
    13681 agatgctggc cggacggaag ccatttgccg aggacgcgca gcactcgctg ccggaaaaaa
    13741 ttcgtaccca ggcgccgtcg tcgcttcaga agctgtgccc cgatgctccg ccggcgcttg
    13801 tgcgcatcat tgatcgctgt ctgcgcaaaa aacgggcgga acgctatcgc acacctgagc
    13861 agctgttggt tgcgctcgaa gggtttgtcg gtgagacggt ggccgagagc ggccaagccc
    13921 acatcgtgcg ttttttggtc gagcgcggac ttttgccacc gggggaactt cagggtctgc
    13981 catcgcccaa gccgcagcgc gctcggcgtc tgacgatccc gcaggtttat cgggcgccgc
    14041 ttgcgcatct tttgctgctc ggtttgttgc tgctgtcggt aacgctgtgg cgcgggctgt
    14101 tttcggccac ttttccgtcg ccgctcgtgc ctgcttggga acccgatgcc aagacagcgc
    14161 agcttttttt gacagccgac ccctgggccg aggtgtatgt cgacggacag cttgtcgaaa
    14221 cgacgccgtt tgcgacgcct ctgcggctgc gcgccggtgt gcgctatttg gagctgcgcg
    14281 gtccgtactt tcgtccggtg cgtcagcgca tcgacctaaa gcctggtgag cgtcgggatc
    14341 ttcacgtcca gatgccgctt ttgcctcggg ggagctagtg cgcgccgcaa gctgggtctg
    14401 gctgtcgctc tgtctttcga tcctgccact gggttttgca caggcagcgt ttccgacgaa
    14461 aaaccccgtc gtgacgcgca aaaatgtggc gatgcccaga ccgcgcgccg tggcatcggt
    14521 tccgcgggaa gaatcgcgtc cagcgcgcga ggatcgtggc cggatcgaga cgcggcttgt
    14581 cgcggcgcgg agcgaatacg cagacgggcg ctatgcggca tcggtgcttt tgctgttgcg
    14641 tgttttggcg gaaacccagc cgtcgctgga tcagcagatt caggtgttcg atctgctggc
    14701 tgcttgctac gtcgcactcg ggcagatcga actggcggtg gggagctttg gcgaggtgct
    14761 gcatcggcgg cctgactttg cgctcgaccc ggtcaagaca tcgcccaaag tgcgggcggc
    14821 gctggaacag gcacggtcgc ggctttagcc agggccgtcc acgccggtca agcgggcgac
    14881 gaagatcccg gggctttgcg cgtttgggca aaggttgggc tgccgcttga acaaagggcc
    14941 gcgtacactc gtcatgaagc gttaggagcc cctttccatg accctgactc tgcgtgcgct
    15001 gctgtgtttt tcgctgctcg gtgtgtcgtt ttcgacggca cgcgctgatg gcccgacgga
    15061 ctatcctggc tacgacgaag gtgatccgaa tcaggtcgtc gacaacggcg acggccaggt
    15121 ctaccagcca cccccggacg gcgtctgttt cgacgacgaa aaccagtcat acgactgtag
    15181 caaagacgaa gactaccaaa gctacagcaa cgtcgatgac ggctacgatc cggcggcgta
    15241 tgaagacttc cgcgatgcgc tcgctcccta tggtcagtgg gtgcaaagtg cgcagtacgg
    15301 cagtgtttgg gtgccgtcgc ctgaatccgt tggagccgac tttacgccgt actattccgg
    15361 cggacgctgg aactacaccg actacggctg gacctgggtt tccgaataca actggggttg
    15421 ggcgccgttc cattatggtc gttggatcac gctgccgggc tacggctgga cctgggttcc
    15481 gggtcgcatc tggggaccgg cgtgggttca ctggcggacg ggtggcggct acgtgggctg
    15541 ggcaccgctg cctccgcgtg gcatggtgat cgccagaccg gtggttggtt cgcgctggca
    15601 ctactggaat ttcgtcgctt cccacgagat catggcgccg cggcttttgc ggattgcgcc
    15661 gccactattg cccaatattt ttgttcggac cgcgattacc aacgactttc gctcgattgg
    15721 ttcgacgcgg gtcatcattg ggccgccgct gcatcacttt ggtggtgccc ggatcgtgcc
    15781 ggcgccgctc gggtcgattc agatttcgct gccgcgctcg cacatcgtgg tgcgaccggg
    15841 cgtgccgctg gtttcgcgca cgtactattc gccgtatcag cgtccgggat ggggatcgac
    15901 gacggtgtcg gcgccgcgac ccggtttccc gccggtctat cgtcgccctg tctatcctgc
    15961 gccgcgccct ggttatcctg cgccgtatcc tcgccctggc tatccagcgg gccagcagcc
    16021 gccggtgtat caaccgccgc cgcgacccgg ttatccgcac ggaccgtcgt atccgcaagg
    16081 gcccggacag catcagccgt ggccgcaagg accagggcag aacctgccgc cgggacagcc
    16141 gccggtgcat cagagaccag gctgggaaaa tcggcccgg
//
LOCUS       DAORGN010000109        16176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1476_length_16176_cov_5.909063, whole genome shotgun sequence.
ACCESSION   DAORGN010000109 DAORGN010000000
VERSION     DAORGN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_16176_cov_5.909063"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKL17_12815"
     CDS             <1..838
                     /locus_tag="PKL17_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45663.1"
                     /translation="GSENGAYARWQGERAGMTDGSSRDPEPEQRKAPGGKERLLLLPD
                     ADLLGLATALAQSSATGAVSAAQLPVDILDGIRQRILRPGTVRRPLHPLLSASCRVTE
                     MEVSDYREKALTVGIVADWASAQGLPSRHVLALEQAVDELLLNALYDAPRDASGSPRY
                     ASVSPADRVFLRALAGESARVRFASDGVRVAVGVADFLGHLRRNTVLDYLIRCATAQA
                     RHASPLEQKKSGAGVGLFLTAMAASELLFRLWRGRLTEIVFCQYLSRPRPLRVLLVDE
                     RL"
     gene            854..2221
                     /gene="hemN"
                     /locus_tag="PKL17_12820"
     CDS             854..2221
                     /gene="hemN"
                     /locus_tag="PKL17_12820"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNK45664.1"
                     /translation="MSATLLHPELLARYGSRPLPRYTSYPPANLWSEQDDSLAQTALA
                     CIGPSPLSLYVHVPFCRKLCLYCGCNMMVTRSQDLVARYLRALDAEVDRVAKLLPSRP
                     EVVQVHLGGGTPTYLDPDQLSTLWQTLTSRFPMARGIEASIEIHPPVTSPDQLSRLAE
                     LGFNRVSMGVQDFDKVVQKRINRPQPFEQTRDLVELSRKLGFVSVNLDLMYGLPLQSV
                     DRFAHTLDRVAELLPDRLALFGYAHMPQLRKHQAVFQRDELPDQKTRAALFELALSRL
                     LALGYVYVGLDHFVLPGDELVAARQKGELRRNFMGYTTCKSSDVLAFGPSSISEVGSH
                     YLQNERDVVPYCERIEQGTLPIVRGYSLCRDDVIRRDLIMQLLCNLEVRLPEFSVRHG
                     IDFASYFAREIEALVPLQNDGLCRLSADSLRIELPGQLLLRTVAAVFDATLSRPAPRH
                     HAAAV"
     gene            2299..2718
                     /gene="hemJ"
                     /locus_tag="PKL17_12825"
     CDS             2299..2718
                     /gene="hemJ"
                     /locus_tag="PKL17_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HNK45665.1"
                     /translation="MLWIKALHVIMVISWMAGLLYLYRLFVYHAMETEEVCRERFKIM
                     ERKLLRGIAHPAGAISVITGVTMLVMNPGYLKMPWMHAKLTFVLGLLISHGQAMYYRQ
                     KLIENPTAYSHKKFRLLNEVPTLLMIGIVIMVIVRPF"
     gene            2804..5185
                     /locus_tag="PKL17_12830"
     CDS             2804..5185
                     /locus_tag="PKL17_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNK45666.1"
                     /translation="MAKRSTDKSSRSGLSLPGQSRRAAHIYRIVRTVILLALLGVCLS
                     MIAVGSIFLYYTSDPSLPTIDTISDYHPKVSTRVLASDGRLIGEIFEERRTVVPRERI
                     PRVIVDAFVDAEDAQFFEHHGLNYVGMVRAVLQSLVQARHLRGASTLTQQLVKTYVLK
                     SNERSLKRKVQEMYLSLRLEKKLSKEEILWLYLTQIYFGHGRYGIEEAARFYFGKSVT
                     DVNPGEAALLASLPKGPEEISPRRNPERAKLRQRYVLSQMARYHHISDAEALKYAEAP
                     ILLTAEPQPMGLFAPEYVEEVEKILVEKYGQEKLPYLGLNVRTACNVEVQRAARLALE
                     QGLVRIDERNGYRKGVRNPSASDRKKHLEELKKEFPSGPPMGRVVEGVVTKVLDGEKN
                     GGWAMVDLGAVVGNLPLPQVGDRYNPKGIAATQRYSEGDVVKVRVGRIGKEGPMLVLD
                     AGPQGAVVVMDPETRQVMAMIGGYGYLRGSFNRVLRAKRQPGSAFKPFVFATAFESRR
                     YTAASVLNDSPQVYDLPDLKAWAPKNAGAHNTTFMGPVRLRIALAQSLNTVASQLMYD
                     LKPPPVAAMAKSLGIESPLEETYALALGASVVTPLELTNAYATLAAKGRRGDPLWILQ
                     VGTEPPQQSDLHQAVTPDLAFLLSHVMQSVIEEGTAVGIKGKLRRPAAGKTGTTNANR
                     DAWFVGYTPDVVAGVWVGFDDSRSLGEKEQGARSALPIWLDVMQVAVKDKRVQNFVQP
                     ASVVIQNIDPVSGKLPAPGAPSIEEVFLAGTEPTEQAPAPGETSAATWNMNQD"
     gene            5382..8468
                     /locus_tag="PKL17_12835"
     CDS             5382..8468
                     /locus_tag="PKL17_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45667.1"
                     /translation="MARIHMAWKKRTQRRALTISSGLLPLLMGIPSAVWSAPQDPPAS
                     AQVQAPSGTETPKPAPQPTPQPTPSPTPQPTPHPVAPPVGTPSASAVGDVPPASVVSP
                     QAPEPTPKEAEAKAKVSGRVVEKATGDGIYDLTVQVVGSELSTTTNEKGEFTLSLEAG
                     SHSLEFVSEFYDRQRISITVPSSGDLVLTAPVAMKLSGTQAEVTAETVVIREPDLKGA
                     AAQLQSRREATTVTDSVSSEDIKKSADSSASQVASRVVGATVVGDRFVYVRGLGERYS
                     STLLHGLPLPSPEPEQHAVPFDIFPAALLSSLSVVKTATPDLPADFAGGSVEIALQEF
                     PTQRVAHVGLGIGGNLQTVFQPMLTQHGGKTDFLGFDDGSRAIPAGKLSGAELGRAFS
                     NNWSPYRQTRGSGNYSLSASFGDRAEVAGKRIGYLFALHYSTDTQTRAEELQVLNLVD
                     TAQGPKLSPLVRFGSDEGAFDQGSGRYSVRTTSGVNWGALGTVAMQLSALHRISLTGL
                     FSQTSDSESRIYQGFSQEQFSDVWNSRLRFIARSLGLVQLRGTHHFEPGQRSPSDLDW
                     AVSYSAAMRDEPDNREVTYLRKDDGLFHFTPKGQSGQRFFAHNLEHQVAARIDFSQSF
                     RQWKGLQSAFKVGAAARVRLRDFSARRFKFGFGGLGDIDDTAAPSDIFSSEHLGSAVD
                     VRENTNTADKYSARQGVYAAYGRVDLPLGSSVSLSGGLRFEAAQQRLYSFDPQNADQP
                     IAVALDTYDPLPSLNLVVKLSAKMNLRAAASMTVARPEFRELAPFQFTDFFGGELVQG
                     NPSLQRTRIGSADLRWEWFLGGADVIALSAYGKYFDAPIETTISAGGNLIRSFANARA
                     AYNFGGELEVRKELAIGERGLRGVSLGGNFTLLYSRVDLDGVAGQQTSKVRPLQGQSP
                     YVVNLFAEFDRAEWGTQLRVLYNVFGERIDQVGAFGLPDKFEQPRHQLDVTLSQRIGR
                     GFSLRLSAKNLINSPVQIVQRGTLPQAGNPDGQKVEETTLRYTTGQTLSLSLSYDL"
     gene            8491..9870
                     /locus_tag="PKL17_12840"
     CDS             8491..9870
                     /locus_tag="PKL17_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45668.1"
                     /translation="MLHISTTKRSLGLWASALCLLAACDDSLVTPNPDPASVTMPTCG
                     GMPAEVLSGTITQNKTLTADKRWLLSGQVKVTQKATLTIEPGTTVCGDATDPQKVSFL
                     NIDQDAKLVADGSREKPIVFTSSRKPGERRTSDWGGVVLRGRAPINLPPGDASACGSL
                     EGNAGSYGPCGTLRSDDSSGVLRYVRIEFAGREVAPDNELNGLTLGGVGSGTVIDYVQ
                     VHRGSDDAFEFFGGTVNVRHLVATGALDDSFDWDQGYSGKGQFLVSQQILADGNNGIE
                     ADNNRDNNSLMPRSSPVFSNVTLIGTGAGSSPKGEKRLGMTLRQGTDGIIQNVIVLGF
                     TEMGVTMTQDSTCTELASGKLTVTAGIFFDNGRDATANLSSVTTPCDVAAALRKSGNR
                     EVNPQLRRPYAQGSSAPDFRPQTSSPALTDAAQQPTDAFFTAAPYLGAFGPADGDDWT
                     QGWTSFPEN"
     gene            10067..10684
                     /locus_tag="PKL17_12845"
     CDS             10067..10684
                     /locus_tag="PKL17_12845"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK45669.1"
                     /translation="MQILKARFHNREEFNEVYHRDLPNGGMFVATTTPLSERDPVVVE
                     LFVKGLPGKVLLRGEVKSWRPALPRLRVRAGALVEFLSDESEKKDFMLAALSGQVVAR
                     KRRHPRLPVTIPVTFHREGALDSLPGELLEISLGGAFLRSDQDLPAVGDDVVVDVLLP
                     GGAVPVPLQGKVATKNESGVGLKFVYRDGGGSRRIRELIRRLKAS"
     gene            10845..11897
                     /locus_tag="PKL17_12850"
     CDS             10845..11897
                     /locus_tag="PKL17_12850"
                     /inference="COORDINATES: protein motif:HMM:NF018239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HNK45670.1"
                     /translation="MRANDIDVAKLLRFVPEKGQLLLGNERFLLFRQDAFGALRKLLY
                     EQLGPELAPALLSQFGYRCGIGDYESLTSQYSWDSEVDRLSAGPTMHCWEGLVRATPT
                     ALSYDRARNQFEMSGVWESSYEAELHIQLFGKSSGPVCHTLTGYASGWATAFFGAPVL
                     AIEPTCVARGDASCSFLLRTPDAFGPEADSWKKNLHFQHASLFEVSRQLAEQIATVQR
                     QDALIRRLSTPVLEVWKDVLAVPIVGAIDEIRSHVLLESVLTAVSQRAARCVILDITG
                     VDVVDAESASYLVKVARAAQLLGTYCVLTGISSAVATKLVETGADLSGVKTLRSLSDG
                     IQACLRILENGVKARR"
     gene            complement(11905..12951)
                     /locus_tag="PKL17_12855"
     CDS             complement(11905..12951)
                     /locus_tag="PKL17_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45671.1"
                     /translation="MSNADNPIQNEPPSEQELFARAEQLLSLGRTADALTLLEQLADA
                     LVDQPQLYYLRALATQQAGGIVSAFVDVRTALALGRRQGIDLHALQSLSDALKSSLPE
                     EIPLLSVSRATANLRRLIAADKQRAAEEIADSLAPHLGSYRPLLLLADGLCRLSRGRP
                     DQAIAPLEAAVSSLPSLWPAAYACGHALLSIGDREGARRAFLHTAEQRQQHPGGFIYS
                     EAEDLLACVPGMAFSSRSFAFERASLLHVLGHSDMALQVLAELIAEEPEASDAHLSQA
                     VLLYQLGRLDDALSALARAEATLRPADRQSEPEDPQRRIELLRLQVLHGLGRHDEANA
                     LAAKLLPPDDTDPR"
     gene            13024..14889
                     /locus_tag="PKL17_12860"
     CDS             13024..14889
                     /locus_tag="PKL17_12860"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNK45672.1"
                     /translation="MTPLAPSLDTLQILARRIRRYSILATTKAGSGHPSSSISAAELM
                     TALFFTALRYDFSDPKRLDNDRFVLSKGHAAPVLYAAWAAAGLISEADLLSLRRLDSI
                     YEGHPTPRIPWIDVATGSLGQGLPNAIGMAAFLKNVVGSPARVWALCGDGEMAEGSNW
                     EAAPIAAELGLSNLTAIVDVNRLEQSVATRLGWDMDRYASQFEVFGWETLVIDGQDLP
                     ACVRAMAHASHSGGSRPLAILARTQKGAGVSFMADHDGWHGKPMDEAHATRALAELGA
                     DDPSVIGKIAKPSGTPRRAPHLERGLTDVAALSFPADKTIATRKAFGEALKRLGDQNP
                     SVMVLDADVKNSTFTEQFEKAHPDRFVQCFIAEQAMVGLAAGAGAISAVPWVATFAAF
                     LSRAYDQIRMAALSSSNLKVCGSHAGVSIGEDGASQMGLEDLAMFRAVHGSTVVYPAD
                     PYATVALVDALSAMHGVAYLRATRGATPVLYSPTEKFPIGGSKTLRQSASDVCVVVAA
                     GITLHEAIKAADQLQSEGIAIRVVDAYSVKPIDADGLRAAAQAGQNRVIVVEDHWIEG
                     GLGDAVLEVFAESGSVRVRKLAVCELPHSGKPEELLDLYGLSARKIAQAVRAFCK"
     gene            14886..15971
                     /locus_tag="PKL17_12865"
     CDS             14886..15971
                     /locus_tag="PKL17_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNK45673.1"
                     /translation="MSDVTNAARAALSEYVALEGVPLAPQLASPQLVRPLGDGLINET
                     FLVELAAPAGPVRAVLQRVNPLFGIAVHDDIEAITTHLAHKGLPTPRLLRTRQGALAV
                     DRGNDGVWRLLSYVAGHSFSQMTPQLAMPAGELVARFHSAVADLDHRFHFVRSGAHDF
                     AKHLRNLRAACEHAAQKDTTPAAFFPLADAIFALADTIPADVPGPLRICHGDLKINNL
                     RFSDDGRGICLLDLDTLASLRLGLELGDALRSWCSPLGEDRSDAYFDTGLLDAVLRGY
                     AGCAKQFVTVEEREFLVTGTLRIAVQLAARFAADVVNQSYFRFDPTRFASRAAHNLVR
                     AQGQLSLAHSLVAQKSVAESLVCRAFS"
     gene            complement(15977..>16176)
                     /locus_tag="PKL17_12870"
     CDS             complement(15977..>16176)
                     /locus_tag="PKL17_12870"
                     /inference="COORDINATES: protein motif:HMM:NF036901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45674.1"
                     /translation="DKTVQAHTELFVPRYQYPDGFSVELSEGTYQYEASKELLIWQTP
                     DLDAELTITISPATNPCPSAV"
BASE COUNT         3088 a         4612 c         5129 g         3347 t
ORIGIN      
        1 gggttcggaa aatggtgctt acgcccgttg gcaaggagaa cgagccggga tgaccgatgg
       61 gtcaagccgc gatcccgaac cggagcagcg aaaggcgcct gggggcaaag agcgtttgct
      121 gctcttgccg gatgcggatt tgctcgggct ggccacggcg cttgcgcagt cttccgccac
      181 gggggcggtt tcggcggcac agctccccgt agacattttg gacgggattc ggcagcggat
      241 tttgcgtcct gggacggtgc gcagaccgct gcatccactt ttgtcggcat cctgtcgcgt
      301 gactgaaatg gaagtctcgg actatcgaga aaaagcgctc accgtcggga ttgtcgcgga
      361 ttgggcgagc gcgcaagggc tgccgtcacg acatgtgttg gcattggaac aagcagtcga
      421 tgagctgctg ttaaatgcgc tgtatgacgc gccgcgtgat gcgtcaggca gtccacgcta
      481 tgcgtcggta agtccggcgg atcgggtctt tttgcgggcg cttgcgggcg aatcggcgcg
      541 ggtccgtttt gcttctgatg gggttcgagt tgccgtcggt gtggccgatt ttttgggaca
      601 cttgcggcgg aatacggtgc tggattattt gatccgctgt gcgacggcgc aggcgcgtca
      661 tgcaagtccg ctcgaacaga aaaaatcagg ggccggggtc gggctgttcc tgacggcgat
      721 ggcggccagc gagctgctct ttcggctgtg gcggggtcgt ctgaccgaga ttgtcttttg
      781 ccaatacctc agcaggccgc ggcctctgcg ggtgctgctt gtcgacgaac gactgtaagc
      841 gagacggtgg acgatgagtg cgacgcttct tcatccggaa cttttggccc gctatggcag
      901 ccggcctttg ccgcgctaca caagctatcc gccggccaac ctgtggtcgg aacaggatga
      961 ttcgcttgcg cagacggcgc tggcctgcat tggcccgagt ccgctgtcgc tgtatgtgca
     1021 cgttccgttt tgtcgcaagc tctgtcttta ctgcggctgc aacatgatgg tgacgcgcag
     1081 tcaggacttg gttgcgcgct atctgcgagc gctcgatgcc gaggtggatc gcgtggccaa
     1141 gcttctgcct tcgcgtcctg aagttgtgca ggttcacctg ggcggtggga cgccgacgta
     1201 tcttgatccg gatcagcttt cgacgctgtg gcagacgctg acgagtcgtt ttccgatggc
     1261 gcgtggcatc gaagcgtcga tcgaaattca cccgcccgtc accagtcctg atcagctttc
     1321 ccgtctggcc gagctgggtt ttaaccgcgt gtcgatgggt gtgcaggact tcgataaggt
     1381 ggtgcaaaag cggattaacc ggccccagcc ttttgaacag acgcgggatc tcgtcgagct
     1441 ttcgcgcaag ctcggctttg tcagcgtcaa cttggacctg atgtatgggc tgcctctgca
     1501 atcggtcgat cgctttgccc acacacttga tcgcgtggcg gagctattgc ccgaccggct
     1561 ggcgctgttt ggttatgccc acatgccgca gcttcgcaaa caccaagcgg tgtttcagcg
     1621 tgacgagctg cccgaccaaa agacccgcgc ggcgctgttc gagctggcgc tgtctcgact
     1681 gcttgcgctc ggttatgtct atgtcgggct cgatcacttt gtcttgccgg gtgacgagct
     1741 ggtcgcggcg cggcaaaaag gcgagctgcg ccgcaacttc atgggataca cgacctgcaa
     1801 gtcgagcgac gtgctggcgt ttggtccgtc atcgatttcg gaagtcggca gccactattt
     1861 gcaaaatgaa cgtgacgtgg ttccgtactg cgagcgcatc gaacaaggaa cgctgccgat
     1921 tgtgcgcggc tactcgctgt gccgtgacga tgtgattcgg cgtgatctga tcatgcagct
     1981 tttgtgcaac cttgaagtgc ggcttcctga attttccgtc cgtcacggta tcgacttcgc
     2041 cagctatttt gcgcgcgaaa tcgaagcgct cgtaccgctg caaaatgatg ggctgtgccg
     2101 cctttccgcc gactcgctgc ggatcgaact gcccggtcag ctcttgcttc gtaccgtcgc
     2161 tgcggtgttt gatgcaacgc tctcgcgtcc tgcgccacgc catcacgcgg cagcggtgtg
     2221 accgattccc aaacgattcc caaacgattc ccaaacgatt cccaaacgat tcccaacaca
     2281 cccaaaggaa ccgatacgat gctctggata aaagcgctcc acgtcatcat ggtcatcagc
     2341 tggatggcgg gtcttttgta cctgtaccgt ctgtttgtct atcacgcgat ggaaaccgaa
     2401 gaagtctgtc gcgagcgctt caagatcatg gaacgcaagc tgctgcgcgg aattgcccat
     2461 ccggcgggtg cgatcagcgt gatcacgggt gtcacgatgc tggtcatgaa tccgggctat
     2521 ctaaaaatgc cgtggatgca tgccaagctg acgtttgtct tggggcttct gataagccac
     2581 ggccaagcga tgtattaccg tcagaaactg atcgaaaatc cgaccgcgta ctcgcacaaa
     2641 aagtttcgcc tgctaaacga agtgccgacg cttctgatga tcggcatcgt gatcatggtg
     2701 atcgtaaggc cattttaacg cgccgtcttc cgtcgccccg gcgtgatttg ctgggcttgg
     2761 gcttttcgca agttggctga cttgcgctat acaagcttag aacatggcca agcgcagtac
     2821 cgacaagagc tctcgcagcg ggctttccct tccgggtcag tcacgccgcg ctgcccacat
     2881 ctatcgcatc gttcgcaccg tcatcctcct ggctcttttg ggagtctgtc tgtcgatgat
     2941 cgcggtggga tcgattttcc tttactacac atccgacccg tcgctgccga ccattgatac
     3001 gatctcggac taccatccga aagtatcgac gcgggtgctg gcgtcggacg ggcgtctcat
     3061 cggtgaaatt ttcgaagagc gtcgcaccgt ggtcccgcgt gagcggattc cgcgcgtgat
     3121 tgtcgatgca tttgtcgatg ccgaagatgc gcagtttttt gaacaccacg gactcaacta
     3181 tgtcggcatg gtgcgggccg tactgcaaag cctggtccaa gcgcggcatc tgcgcggcgc
     3241 atcgacgctg acccagcagc ttgtgaagac gtatgtgctg aaaagcaacg aacggagtct
     3301 caagcgcaaa gtgcaggaaa tgtatctttc gctgcggctg gaaaagaagc tgagcaaaga
     3361 ggaaatcctg tggctgtatc tgacccagat ttactttggg cacggtcgct acggcatcga
     3421 agaagcggcg cggttttatt ttggcaagtc agtcaccgac gtaaatccgg gtgaagcggc
     3481 gcttttggca tcgcttccca aaggtccgga agaaatctcg ccgcgtcgca atccggaacg
     3541 ggccaagctg cgtcagcgct atgtcctttc gcagatggcg cggtatcacc acatctcgga
     3601 tgccgaagcg ctcaagtatg cggaagcgcc gattctgctg accgccgagc cgcagcccat
     3661 gggtctgttt gcaccggaat atgtggaaga ggtcgaaaag atcctcgtcg agaaatacgg
     3721 tcaggaaaag cttccgtacc tgggcctgaa tgtccgcacc gcttgcaatg tggaagtgca
     3781 gcgcgcggcg cggctggcgc tggaacaggg tcttgtgcgc attgatgaac gcaacggcta
     3841 ccgcaaggga gtccgcaacc caagcgcaag tgatcgcaaa aaacacctgg aggaactcaa
     3901 aaaggaattt ccttccgggc ccccgatggg gcgggtggtc gaaggcgttg tgaccaaagt
     3961 tctcgacgga gaaaagaacg gcggctgggc gatggttgat ctgggcgcgg tggttgggaa
     4021 tctgccgctt ccgcaagttg gggaccgcta caatcccaaa ggaatcgccg ccacccagcg
     4081 ctattccgaa ggcgatgttg tcaaagtgcg agtgggtcgc attggcaaag aaggtccgat
     4141 gctggtgctg gatgccggac cgcaaggtgc cgtggtggtg atggaccccg aaacgcggca
     4201 agtgatggcg atgatcggcg gctatgggta tctgcgcggc agcttcaacc gcgtactgcg
     4261 ggccaagcga cagcccggat cggcgttcaa accgtttgtt tttgcgacgg cctttgagtc
     4321 ccggcgctac accgcggcga gcgtgctaaa tgactcgcca caggtctacg atctgccgga
     4381 tttgaaagcg tgggcaccga agaacgcggg cgcgcacaac acaaccttta tgggtccggt
     4441 gcggctgcgg attgcgctgg cccagtcgct caacaccgtg gcatcgcagc ttatgtacga
     4501 cttgaaaccg ccgccggtgg ccgcgatggc caagtcgctg ggaatcgagt ccccgctcga
     4561 agaaacctat gcgctggcgc ttggtgcgtc ggtggtgaca ccgctggaac tgaccaatgc
     4621 ctatgcgacg ctggcggcga aaggtcggcg cggcgatccg ctgtggattt tgcaagtggg
     4681 gacggaaccg ccgcagcaaa gtgatctgca ccaagcggtg acaccggatc tggcgttcct
     4741 tttgagccat gtgatgcaga gcgtgatcga agaaggaacc gcggtaggaa tcaaaggcaa
     4801 gctgcggcga ccggcggcag gaaagaccgg aacgaccaac gccaaccgcg atgcctggtt
     4861 tgtcggttac acccctgatg tcgtcgctgg cgtgtgggtt ggttttgatg actcgcgatc
     4921 acttggggaa aaggaacagg gggcgcgatc ggcgctgccg atttggctgg atgtgatgca
     4981 ggtggcggtc aaagacaagc gggtgcaaaa ctttgtccag cccgcttctg tggtcatcca
     5041 gaacatcgat ccggtgtccg gcaagcttcc cgcgcccggt gccccaagca tcgaagaagt
     5101 ctttttggcc ggaacagagc ccaccgaaca agcgcccgcc cccggtgaaa ccagcgccgc
     5161 aacctggaac atgaaccagg actaaccgac gtgtttccgt ccagatccga ttcctacgcc
     5221 cgcttgttgc ctcgctgaca cagtccgagt tgttacgctg ttgacacaat gggaatgtca
     5281 cccaattgac acaatctgga atgtcaccca attgacacaa tctcgtcacg gacttgcccc
     5341 aagcgatcga ttcctgatgc ctatacttcg agccaaacaa cgtggctcgc atacacatgg
     5401 catggaaaaa acggacgcag cgtcgggctc tgacaatttc aagtggtctt ctgccgctgc
     5461 ttatgggcat tccatcagcg gtttggtcgg ctccacaaga tccaccggca tccgcgcagg
     5521 tgcaggcacc gtcaggaaca gagactccga aaccggctcc gcaaccgact ccgcaaccga
     5581 ctcctagtcc gactccgcaa ccgactccgc atcccgtggc gccacccgtt gggactcctt
     5641 cggccagcgc ggtgggtgat gttccgcctg cttccgtcgt ctctccacaa gcgccggaac
     5701 cgacccccaa agaagcagag gccaaggcca aagtcagcgg tcgtgtcgtc gaaaaagcca
     5761 ccggagacgg tatttatgat ctcacggtgc aggttgtggg aagtgaactg tcgacgacca
     5821 cgaatgaaaa gggagagttt acgctttcgc ttgaggcggg ttcccattcg ctggagtttg
     5881 tatcggagtt ttacgaccgc cagcgcattt cgatcactgt gcccagcagt ggggacttgg
     5941 ttctgacagc gcccgtcgcc atgaagctga gcggcaccca agccgaagtg actgccgaaa
     6001 ccgtggtgat tcgcgaaccg gatctgaaag gggccgcggc gcagcttcag agccgtcggg
     6061 aagccaccac ggtgaccgat tctgtgagca gcgaagacat caaaaaatca gctgatagca
     6121 gcgcttccca agtcgcaagt cgtgttgtcg gtgccaccgt ggtcggggat cgctttgtct
     6181 atgtgcgcgg cctgggtgaa cggtattcga gcacgctttt gcatggtctg cctctgccaa
     6241 gtccggaacc ggagcagcac gcggttccgt tcgacatttt cccggcggcg cttctgtcga
     6301 gcctgagtgt tgtaaaaacc gcaaccccgg acttgcctgc cgactttgcc ggtggttcgg
     6361 tggaaatcgc gctgcaagag ttccctaccc agcgcgttgc tcacgtcggt ttgggaatcg
     6421 gtgggaatct gcaaacggtg tttcagccga tgctgacgca gcacggtggg aagacggatt
     6481 ttctcgggtt tgacgacgga tcgcgggcga ttccggcagg caagctgagc ggcgcagagc
     6541 tgggccgggc gttttccaac aactggtccc cgtatcgcca gacccgcgga tcgggaaact
     6601 attcgctgtc ggcttccttt ggcgatcgcg cggaagtggc cggcaagcga attgggtatc
     6661 tgtttgcgct tcattacagc accgacaccc aaacgcgcgc cgaagaactg caagtgctga
     6721 acttggtcga caccgcgcag gggcccaagc taagtcctct tgtccggttt ggcagcgacg
     6781 agggggcatt cgatcaggga agcggaaggt attctgtccg gaccacttcc ggcgtcaact
     6841 ggggcgcgct gggaacggtg gcgatgcagc tttctgcgct gcatcggatt tcgctgaccg
     6901 gactgttttc gcagacctcg gacagtgaat cgcgcatcta ccaaggcttt agccaagagc
     6961 agttttcgga tgtctggaac agccgcttgc gtttcatcgc gcgcagcttg ggacttgtgc
     7021 agttgcgtgg tacacaccac ttcgaaccgg ggcagcgcag tccgtccgat ctcgattggg
     7081 cggtgagcta ttcggcggcg atgcgcgacg agccagacaa ccgcgaagtg acgtatctgc
     7141 gcaaagacga cggactgttt cacttcacac ccaaagggca gagcggtcag cgtttctttg
     7201 cccataactt ggaacatcaa gtcgcggcgc ggatcgattt ttcgcagagc tttcggcagt
     7261 ggaaaggact gcaatcggcg ttcaaagtcg gcgcggcggc gcgggtgcgg ctgcgtgact
     7321 tttccgcgcg gcgcttcaag tttggctttg gcggactcgg tgacatcgac gatacggcgg
     7381 ctccttctga catcttttca tccgaacatt tgggcagcgc ggtcgatgtg cgcgaaaaca
     7441 ccaacaccgc agacaagtac agcgcgcggc aaggtgtcta tgcggcctat ggtcgtgtcg
     7501 atctgccgct cggcagcagt gtgagtctgt ctggcggact ccgtttcgaa gcggcgcagc
     7561 agcggctgta ttcctttgat ccacaaaacg cggatcagcc gattgcggtg gcactggata
     7621 cttacgatcc gctgccgtct ttgaacttgg tggtgaagct ctctgcgaag atgaacttgc
     7681 gcgcagcagc gtcgatgacc gtcgcacgtc cagaattccg ggagctggct ccgtttcagt
     7741 ttaccgactt ctttgggggc gagctggtgc aaggaaatcc gtcgcttcag cgaacccgaa
     7801 tcggcagcgc cgatctgcgc tgggagtggt ttttgggcgg cgccgatgtg atcgctctgt
     7861 cggcttacgg aaagtacttt gatgcaccga tcgagacgac gatcagcgca ggcggcaacc
     7921 tgatccgcag cttcgccaat gcacgcgcgg cgtataactt tggtggggaa ctggaagtcc
     7981 gcaaagagct tgcaatcggc gaaaggggtc tgcgcggagt gtctttggga ggcaacttca
     8041 cgctgctcta ttcgcgggtt gatctcgacg gtgtggctgg ccaacagacc agcaaagtgc
     8101 gtccgctgca agggcagtca ccctatgtcg taaacctgtt tgcagagttc gatcgcgcgg
     8161 agtggggcac ccagcttcgc gttctttaca acgtgtttgg cgagcggatc gatcaagtcg
     8221 gtgcgtttgg tctgccggac aagttcgagc agccacgcca tcagcttgat gtgacgctgt
     8281 cgcagcgcat aggtcgcggt ttttcgctgc ggctgtcggc caaaaacttg atcaattccc
     8341 ctgtccagat cgtccagcgc ggcacgctgc cgcaagccgg aaaccccgac ggacagaagg
     8401 tggaagaaac caccctgcgc tacaccaccg ggcagaccct gagtctgtca ctttcctatg
     8461 acctgtaagg ccaaaaagga gacatgtaaa atgcttcata tttcaacgac aaagcgctca
     8521 cttggactgt gggcttcggc gctctgtttg cttgcggcgt gtgatgacag cctggtgact
     8581 cccaatcccg atccggcgtc agtcacgatg ccgacgtgcg gtgggatgcc ggcggaagtg
     8641 ctcagcggca ccatcacgca gaacaagaca ctgaccgcgg acaagcgctg gctgctgtct
     8701 ggacaggtca aagtcaccca aaaagcgacg ctgaccatcg aacccggcac caccgtgtgc
     8761 ggcgacgcga ccgatccgca aaaggtcagc tttctgaaca ttgatcaaga cgccaagctg
     8821 gttgcggatg gcagccgcga aaaaccaatc gtatttacgt cgagccgcaa gcccggtgaa
     8881 cgtcgcacga gcgactgggg tggcgtggtg cttcgtggtc gcgcccctat caacctgccg
     8941 ccaggggatg caagtgcgtg cggcagcctg gaaggaaacg ccggttcgta tggtccctgc
     9001 ggaacgctgc gcagcgacga ttcaagtggt gtactgcgtt acgtgcggat cgagtttgct
     9061 ggtcgggaag ttgcaccaga caacgaactc aacgggctga cgctgggggg cgtcggcagc
     9121 ggcacagtga tcgattacgt gcaggttcac cgcggatcgg acgatgcatt cgagttcttc
     9181 ggcggcaccg tcaatgttcg tcacctggtc gcgacgggcg cgctcgacga ttcgtttgac
     9241 tgggatcagg gctattctgg aaaaggtcag tttctggtct cgcagcagat cctggccgac
     9301 ggcaacaacg ggatcgaagc cgacaacaac cgcgacaaca actcgcttat gccgcgatct
     9361 tcgccggtgt tttcgaacgt gacactgatc ggtacgggcg ccggcagcag tcccaaaggc
     9421 gaaaagcgct tgggcatgac gctgcgccaa ggcaccgacg gcatcatcca aaacgtgatc
     9481 gtcctgggct ttaccgaaat gggcgtgacg atgacccagg acagcacctg caccgagctg
     9541 gcgtcgggga aactgacggt gacggcgggc attttctttg acaacgggcg cgacgcaact
     9601 gcaaaccttt cgtcggtgac gacgccttgt gatgtggcgg cggcgctgcg caagagcggc
     9661 aatcgtgaag tcaatccgca gctgcgacga ccctatgcgc aaggaagcag cgcgccggat
     9721 ttccgtccac agaccagctc gccggcgctc accgacgcag cgcagcagcc gacggacgct
     9781 ttcttcacgg cggcaccgta tctgggggcg tttggtcctg ccgacgggga tgactggacg
     9841 cagggctgga cttcattccc cgaaaactaa ccgcaaaacc tccgcaggcg gctgcgcggg
     9901 cgcgtttgtg cggcattcat tgcttccaaa ttctggaagc tagattccag aatatgaatg
     9961 acgcaatcgg cttgcagcga cgcagatcgg cgggcctctc ctgcgccgcg aggtttcctg
    10021 tactacagct tgtacccaag acgctttgtg atatggtccg cgcgccatgc agatcctgaa
    10081 ggcccgcttt cacaatcgcg aggagtttaa cgaggtctat caccgtgacc ttcctaacgg
    10141 cggaatgttc gttgcgacga caacaccgct gtcagaaaga gatcccgtgg tggtggagct
    10201 gttcgtaaaa ggactccctg gaaaagtctt gctccgcggc gaggtcaagt cgtggcgtcc
    10261 ggcgctgccc cgtctgcgcg tgcgcgctgg agctctcgtc gagtttctgt cggatgaatc
    10321 cgaaaagaag gacttcatgc tggcggcgct gtcgggccaa gtggtggcac gcaagcggcg
    10381 gcatcctcgt ctgccggtga caattccggt gacgttccat cgcgaaggcg cgctcgacag
    10441 cctgcccggc gagctgctcg aaatttccct tggtggtgcg tttctgcgca gcgaccagga
    10501 tctgccggcg gtcggtgacg acgtggtggt agatgtactg cttcccggtg gtgcggttcc
    10561 tgtaccactg caaggcaaag tcgccaccaa gaacgaatcg ggcgtgggtc tgaagtttgt
    10621 ctaccgcgat ggcggtggtt cccgtcggat tcgcgagctg attcgtcgtc tcaaagcatc
    10681 gtaagcgctg tttcctcgtg tgccgaaagt gcgcctttct gcgcgcaagc ggtgcgtcgt
    10741 cccctccgtg attaccgtct gaatcgtcga atttcccgtc gaaacgcgag cggatgatcg
    10801 attactatcg atccgcggtt tccgtccgat tccgctccaa aggcatgcgt gcaaacgata
    10861 ttgatgtcgc gaagctgctg cgcttcgttc ctgaaaaggg gcagcttctg cttgggaatg
    10921 agcgattttt gttgtttcgg caggatgcgt ttggcgcgct gcgcaaactg ctttacgaac
    10981 agctcggtcc agagctggca ccggcgctgc tttcgcagtt tggctaccgc tgcggaatcg
    11041 gtgattatga gtcgctgact tcgcagtata gctgggattc cgaagtggat cggctgtccg
    11101 ctgggccgac gatgcactgc tgggaagggc tcgtgcgggc aaccccgacg gcgctttcat
    11161 atgatcgggc ccggaaccag ttcgaaatgt cgggtgtgtg ggaaagctcg tatgaagccg
    11221 agctgcacat tcagcttttt ggcaagagct cggggccggt ttgtcacacc ttgaccggct
    11281 atgccagcgg ctgggcgacg gcgttttttg gggccccggt gctggcgatc gaaccgactt
    11341 gcgtggcgcg tggcgatgcg agctgttcat tccttctgcg cacgccagat gcgtttggtc
    11401 ccgaggctga ttcgtggaaa aagaacctgc actttcagca tgcctcgctg tttgaagtgt
    11461 cgcgacagct ggccgaacag atcgcaaccg tgcagcggca agacgcgctc atccgacgtt
    11521 tgtcgacgcc cgttctggaa gtttggaaag atgtgctggc ggttccgatt gtcggcgcga
    11581 tcgacgaaat tcgaagccac gttctgttgg aatcggtgct gaccgcggtg tcgcagcgtg
    11641 ccgctcgctg cgtgatcctc gatatcaccg gcgtcgatgt ggtggatgcc gagtccgcca
    11701 gctatctggt caaggtggcg cgggcggcac agcttctggg gacgtactgc gtgctgacgg
    11761 ggatttcgtc cgcggtcgcg accaagctgg tggaaaccgg ggcggatcta agcggcgtca
    11821 agacgctgcg ctcgctcagc gatggaattc aagcttgtct gcgtattttg gaaaacggcg
    11881 tcaaagcccg tcggtaagcc ggacctaacg cggatcggta tcgtcggggg gcagcagttt
    11941 ggcggccagc gcgttggctt cgtcgtggcg accgagcccg tgcagcacct gcaagcgcaa
    12001 aagttcaatc cggcgctgcg gatcttcggg ttccgactgg cgatcggcgg ggcgcagcgt
    12061 cgcttcggcg cgtgccagcg ccgagagcgc atcgtcaagg cgtccaagct gatacagcag
    12121 cactgcctgc gataggtggg cgtcggatgc ttcgggttcc tcggcgataa gctcagcgag
    12181 cacttgcagc gccatgtcgc tgtgacccaa gacgtgaagc aggctggcgc gctcaaatgc
    12241 aaagctgcgc gacgaaaacg ccataccggg cacgcaagcg agcagatcct cggcttcgga
    12301 atagatgaaa ccgcccggat gttgctgtcg ctgttccgca gtgtgcaaaa acgcccgtcg
    12361 ggcaccttcg cggtcgccta tcgaaagcag cgcgtgacca caggcatagg ccgcaggcca
    12421 caaagacggc agcgacgaga cggcggcttc gagtggcgcg atcgcttgat cgggacgacc
    12481 gcggcttaat cggcacagac catcggcgag cagcaagagt ggtcgatagc tgcccagatg
    12541 cggagcgagc gaatcggcga tctcttcggc ggcgcgctgc ttgtcggcgg cgatcagccg
    12601 gcgcagattt gccgtcgctc gcgacaccga caagagcgga atttcttcgg gcaaagacga
    12661 cttgagcgcg tccgatagcg actgaagcgc gtgcaagtcg atgccttgcc gtcgtccaag
    12721 cgccagcgcg gtgcgcacgt cgacgaacgc cgacacgatg ccgcccgcct gctgcgtcgc
    12781 cagcgcacgc agataataca gctgcggctg gtcgacaagc gcatcggcaa gctgttcgag
    12841 cagcgtcagc gcatctgccg tccggcctag cgaaagaagc tgctcggccc gcgcaaaaag
    12901 ctcctgctcc gacggtggtt cgttctgtat agggttgtct gcgttactca tgacacgaag
    12961 ctgggacaga tagtgtacca tccgccgcgc tttcgactaa aacctccaag gagttcgtcg
    13021 gacatgaccc ctttggcccc ctcgctcgat acgctccaga ttcttgctcg tcggattcgt
    13081 cgctattcga tcctggccac caccaaggcg ggatcggggc acccttcgtc gtcgatttcg
    13141 gcggcagagc ttatgacggc gctgtttttt acggcgctgc gctatgactt ttccgatccc
    13201 aagcggctcg acaatgatcg cttcgtgctc tcgaaggggc atgcggcgcc ggtgctgtat
    13261 gcggcgtggg cggcggcggg gcttatcagc gaagcggatc ttctgtcgct gcgacggctc
    13321 gatagcatct atgaaggcca tccgacgccg cggattccgt ggatcgatgt cgcgacggga
    13381 tcgcttggcc aaggtctgcc caatgcgatc ggaatggcgg catttttgaa gaatgtcgtt
    13441 ggctcgccgg cccgtgtgtg ggcgctgtgt ggcgacggag aaatggcgga aggatcgaac
    13501 tgggaagcag cgcctatcgc ggcggaactc ggtctttcca acttgacggc gatcgttgat
    13561 gtgaaccggc tcgaacagag cgtcgcgacg cggctcggct gggacatgga tcgctacgct
    13621 tcgcagtttg aggtgttcgg ctgggaaacg ctggtgatcg acggacaaga cctgccggcg
    13681 tgcgtgcggg cgatggccca tgccagccac agcggcggca gtcgtccgct ggcgatcttg
    13741 gcgcggaccc aaaagggcgc cggggtgtcg tttatggccg atcacgacgg ctggcacggc
    13801 aagccgatgg acgaagcgca tgcgacgcgc gcgctcgccg agctgggcgc agacgatccc
    13861 agcgtcatcg gcaagattgc caaaccgagc gggacgccgc gacgggcacc acatctggaa
    13921 cgcggactta ccgacgtggc ggcgctgtct ttcccggcgg acaagacgat cgcgacgcgc
    13981 aaggcgtttg gcgaagcgct gaagcgactg ggggatcaga atccgtcggt gatggtgctt
    14041 gatgccgatg tgaaaaattc gacgtttacc gagcagtttg aaaaagcgca ccccgatcgc
    14101 tttgtgcagt gcttcatcgc cgaacaagcg atggtcgggc tcgcggcggg cgctggtgca
    14161 atttctgccg tgccgtgggt ggcgacgttt gcggcgttct tgtcgcgcgc ctatgaccag
    14221 attcggatgg cggcgctgtc gtcgtccaat ctcaaagtct gcggctcgca cgcgggcgtc
    14281 agcatcggcg aagacggcgc aagccaaatg ggtctggaag acctggcgat gttccgcgcc
    14341 gtgcatggat cgacggtggt gtatcctgcg gacccttacg caacggttgc gctcgtcgat
    14401 gcgctgtcgg cgatgcatgg cgtggcgtat ctgcgagcga cgcgcggcgc gacccctgtg
    14461 ctgtacagcc cgacggaaaa attcccgatc ggcggcagca agacgctgcg gcagagcgct
    14521 tccgatgtct gcgtggtcgt cgcggcggga attacgctac acgaagcgat caaagcggcg
    14581 gatcagctcc agtcggaagg catcgcgatt cgcgtcgtcg atgcgtactc agtcaagccg
    14641 atcgatgccg atggactgcg cgcagcggca caggccggcc aaaaccgcgt cattgtcgtc
    14701 gaagatcact ggatcgaagg tggtctcggc gatgcggtgc tcgaagtgtt tgctgaaagt
    14761 ggctcggtcc gggtgcgcaa gctggccgtc tgtgaactgc cgcactcggg caagcccgag
    14821 gaactgctcg atctgtacgg tctgtcggcg cgcaagattg cgcaagcggt gcgggcgttc
    14881 tgcaagtgag cgacgtgacc aatgcggcgc gggctgcgct ttccgaatat gttgcgctcg
    14941 agggcgtgcc gcttgcgccg cagcttgcgt ccccacagct tgtccgtccg ctgggcgatg
    15001 gcctgatcaa cgagacgttt ctcgtcgagc tggccgcgcc tgcgggccct gtgcgggcgg
    15061 tgcttcagcg cgtcaatccg ctgttcggga tcgccgtcca cgacgacatc gaagcgatca
    15121 ccacgcacct tgcgcacaag ggtctgccga cgccacgcct tctgcggacg cggcaaggcg
    15181 cgctcgctgt cgatcgggga aacgacggtg tctggcggct gctttcctac gtggcgggac
    15241 attccttttc gcaaatgacc ccgcagcttg cgatgccagc cggagaactg gtcgcgcgct
    15301 ttcattccgc ggtcgcggat ctggaccacc gctttcactt tgtccgcagc ggtgcgcacg
    15361 actttgccaa gcacttgcga aacttgcgtg cggcctgtga acacgctgcg caaaaagaca
    15421 ccacacctgc cgcgttcttt ccgctcgcgg atgccatctt cgcgctggcg gacacgattc
    15481 ctgcggatgt gccggggccg ctgcggatct gtcacggcga tctgaagatc aacaacctgc
    15541 gcttttccga cgatggccgc ggaatctgtc tgctcgatct ggatacgctg gccagtctgc
    15601 ggctgggact tgaactgggt gatgcactac gttcctggtg cagtccgctc ggcgaagatc
    15661 gcagcgatgc gtattttgac accggactgt tggacgcggt gctgcgcggt tatgcgggct
    15721 gtgccaaaca gtttgtcacg gtggaagaac gcgagtttct tgtcaccggg acgctgcgca
    15781 ttgcggtcca gttggcggcg cgctttgcag ccgatgtcgt aaaccaaagc tacttccgct
    15841 ttgatcccac ccgctttgcc agccgcgctg cacacaactt ggtgcgcgcg caaggccagc
    15901 tttccctggc gcacagcctt gttgcgcaaa aatccgtcgc tgaatccttg gtctgccgcg
    15961 cgttttccta aacggcctac accgcggacg gacagggatt ggtggctggc gaaatggtga
    16021 tcgtaagctc tgcatccaga tcaggagtct gccaaataag aagctctttg gatgcttcat
    16081 attggtaggt tccttccgat agctcgacag aaaaaccgtc cggatattga tagcgcggca
    16141 cgaagagctc ggtgtgggcc tgcaccgttt tgtcac
//
LOCUS       DAORGN010000110        16176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1477_length_16176_cov_5.091185, whole genome shotgun sequence.
ACCESSION   DAORGN010000110 DAORGN010000000
VERSION     DAORGN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1477_length_16176_cov_5.091185"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1708
                     /locus_tag="PKL17_12875"
     CDS             35..1708
                     /locus_tag="PKL17_12875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45675.1"
                     /translation="MSTSTSEERLLLEYLQQRHGLRMEQAEQHHRAWQNLDSDGKRAP
                     RPFADFLVEKQLLDRVTAKTLKAMRMGYLSASDAEMRSTLGLTAAPETKVAQAPQVEK
                     QESPVVPKEAPVLPRDTPGAKAEVAVPAAQSRSAIPDVSIPLVRSDAEIPAIEIVAST
                     SQDTKALRAEHVPENRPSPDHPESSRLQVGQSLGRYDLEEVLGEGSTAVIYRSYHKLL
                     QMPVAIKRFKPAVLSRRMGSRERIVQEGRLMVRMDHPNIVRVLDIDESDGVPYIVFEY
                     VSELSLQALIDNIGRLSSERLAQIGAQVAAALSAASSAGLLHRDVKPENILVRKDGLI
                     KLADFGIATYLLPDGTTVDELARAGLISGTPQYISPEQISTPAQIDFRADIYSLGATL
                     YHAATGHPPFDFSTLDELLNAHLAMAPLPLASLVPGIDAELAQLIAQMMQKRPEDRPQ
                     SLTELSEKFERIADRVLHSESVHRKNVIATQSSSHPVIAVDGAASTQVGRAVESTVVH
                     EEKPSLKNRNSAPFVVDLSVLWGRNIAIPLILLVCVLGIALVLWFAVVR"
     gene            1724..2458
                     /locus_tag="PKL17_12880"
     CDS             1724..2458
                     /locus_tag="PKL17_12880"
                     /inference="COORDINATES: protein motif:HMM:NF017779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45676.1"
                     /translation="MQRVSVSSILSALQQDCASFLRDSSYTPVPIPAHFSEMKGTLRG
                     ENITLNTRRFLGHHYRSITMAWIVRDSDGSVCSLTVIGLPTQDSGAPILGVDLIGLGG
                     SLSLVAIDLAPTDDATYSERSMPRLRSLHSMTDDSVVHRKRPEFCQDTFSPLALICAG
                     KPGHEAQLQSACATFLSMELDARKQTSPFVVLPAERADSAASKQRAWLLSERKNRKEA
                     KYMSLMFGEDLAADYLYNFLFEVPSV"
     gene            2451..3161
                     /locus_tag="PKL17_12885"
     CDS             2451..3161
                     /locus_tag="PKL17_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HNK45677.1"
                     /translation="MCNPSIGATEPLSVRLRTGTRKVHRSVEKVQFIRSFLKGMLHRE
                     SYLQYLRDLYVVYSALEQQLSFHNRNPKLSALFLPALFRSAALRRDLICLAGPDFERR
                     ISPSPVAIEYARALVRLSHTQPYLLSAHAYTRYLGDLSGGQVLHKMAQLGLGISGPEG
                     LSFYQFPLVSDVPAMRVMYRQQLDLLPLSGDEMTAIVDEACRAFAWNQSLLVQLPVPA
                     VRFSETPRFVAQAIQNAG"
     gene            3179..5227
                     /locus_tag="PKL17_12890"
     CDS             3179..5227
                     /locus_tag="PKL17_12890"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45678.1"
                     /translation="MSPASVPIPDNASQAAVGEGTELGRYRLIRKLGEGGMGVVYEAE
                     HRELGRKVAIKVLRTGTTSSDIQAARFQREAELVSRIGHPNIVEIYDFGRTSDGNLYF
                     VMELLHGESLRSRLRTRPLSDQEIAEIFAPLLSAIAAAHSQGIVHRDLKPDNVMLGDA
                     GVKLLDFGVAKNLSQQGADMTTVSGALLGTPAYMAPEQIRHSSHVDARADIYAAAVML
                     YEALTGQRPFVGNAIELLSKQLFESVVPPSEVARQQRIDRPNIDWPTMDRIVLRGLCK
                     EPEGRYPEVGTFLHELQTAWPRVEVPDLSGKHSLGETAASGNSIPEDHRSSWLQRLQM
                     VRLVALGAVLALLGAGLLWRLLRNPMPSQKPSMQVQAQSPTDSEHSPAERAQQRILAA
                     LAGPREERRALALAMADVGGSALHDRLLQLLRDPEPAVAKAALQAAGSYGVAVDPAMI
                     AALKQLGEQAGGALGVDAMTARLGLGDTTVVAGLSAAVAKPSLDVEVRLRAQLALVQA
                     GALKPSALRAAVLQSVEQGGLRPTLYRRALRELLRLKDPGTVKRLDRALTDANPNLRS
                     EAAEVLSLLGRRDGQDALYQLAMQHPDELSFRTLLAESGDVRAKELLRPRLSDPSGMV
                     RQKAVAALAALSVHGGRPCDVSLVVPLLRDADALVRLSAAVAFVQLDRCQRAEATATP
                     "
     gene            5224..8592
                     /locus_tag="PKL17_12895"
     CDS             5224..8592
                     /locus_tag="PKL17_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK45679.1"
                     /translation="MSFDPQSLIGQTVGEFKLTALLSRTDSVIRYRAEHQKLRRVATC
                     HVLDPRETSSHGQAFLDGARRISQIRQRNLIDIFDSGTILDSPYFVSEELLGEPLLTW
                     CSRHRFSVSRLFRIAQQVEAALLAIDQAGAAVSALSPDSLFVRRVAGSLQVKLVDVTL
                     VPVHKSNEQSPTHISISVAATFLFGLVQAQPFAQPLPALPLSRRSLFDGVGRLSGLGD
                     DLEPVLRRGLGIETPPFDSLRSFLSAALATIPKRAPLPLENVRAALRKPRTWLLAGPL
                     ILLLLVPLALLLHRPRTKPPVAEKTYTVRELHDLARTTLEAGLRSPLPLVREQALTGL
                     LVGADIGWQAQVEPLLDDPSPSVQTRAAEVLGGLGSRRTLPTLEAHLGREYDPIVRAT
                     SSQSIHKLGKSVNRAAVSSLLTGDNVRAKRIAAQLLAEQGDAEAERLVAELQGQTGEE
                     AQEARLWAARRGQDAAQALLRSSLPKTPPLDVKDLPVAELFLRQEVAESRELLVATAK
                     QPGPAQTRAAQILCKNENSTFLPLLRDVVVDAGRALPERLVAASGLARCGERRDGQLL
                     ARLLLDGATNERLKQGCAGALLALTSRDPDLSAQQNMAWAKQALDDESWSVRESAVAL
                     LGESETQEPERQAVAKRTETAESAKPAPTPQVVQLLVRAMDDAALPVRMTAMRSAVKV
                     ARKSQKPSEQDSLKQSLRKRAETGLPEEQIVAAASLLRIGDASYRDTLHKGLRAAEVA
                     VRRRAIEEADADPKLPSKLLVPLTNDQDFAVRFGAAALLVAQGQKKPALVQTLREAVQ
                     RGGSEGVRAYALLQKLGELSPQTESKAPDLDTLLASSNVLERLMAIDALQKLPAEEAA
                     PLLLKATRDPDSTVRFRLVDVVAHHCQPGPSQRAALPALRALAEDHDVAVRARAFELL
                     SQLQATLSKDGDAGPDAAKADGKTDSAAGNSENAMGESPPALSPEAARKSQSVDQQFA
                     SGSALFKAGNYRGAQKALEKTSQMCTAYRGDVPKCTQIGSNLSYQLGVTYENLGQLAS
                     AMTEYQKVLQGTGGGKGGKRPRGLAISDAAQQGVERLRGKLGRLVLYKTLAGKCQKVE
                     VYMPPGKHLVNVGGAVRKPVELDAGETVELRNCR"
     gene            8609..10132
                     /locus_tag="PKL17_12900"
     CDS             8609..10132
                     /locus_tag="PKL17_12900"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK45680.1"
                     /translation="MVLILLVLGMAQGASGKGPRRGAVKATPAVLREKVQAALARSDF
                     AEAHKLVDDSYRQAPQPEKLFLLAQVAQAEGQAQAARDLFRRFLADMGTEGPQSQKLA
                     QSAVAGPLLPSGEVNVEGDRGALVYVDDHPVGVLPLLTPLWLSTGAHKISVLSGSKRQ
                     EEQYKVLAARKADLRFNLSSDVVVATLQPAALLVLFPTASEGQKTLSLSPDEVSALEQ
                     GAQSALSGKPLGLQTQAAALLAAPHLANCLADWNCQKTLGEENQANWLLLLSVEVTQR
                     WRGARDLKLRLAAHDPAIAEPAASAERMCTACTADKAKQIATELFGVVVPEATSRPRG
                     TLIVQSVPDGAEVHLGARLMGHTPLKRTVWAGTYPLVISKRGFSPLQREVTVTAGQRQ
                     TVEVTLLLGTLGDATKVPSSLLPEYSEEPSNPAKTAAGQRPRWRIVTGASAVVLGAVT
                     VGFGISALAVNNHCVAGLQDASMQCGQIYDTLPPGAALTSVGGVALLAGSMLLAWPG"
     gene            complement(10152..10655)
                     /locus_tag="PKL17_12905"
     CDS             complement(10152..10655)
                     /locus_tag="PKL17_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45681.1"
                     /translation="MSRPARPLQITQQMLSLRRMAEAEAQAELRAARAELERVRHSEQ
                     AAGQRRQQMAKMLAAAEKTAQSTRTIAANELAEQAMRLQLLRGELRRAEADEAARGHQ
                     VNQLRRLIEELQAHLRLCVARREAAEQHEAAERREQRRQRERRERAIEEDARDRVLSA
                     SRPHRRA"
     gene            complement(10740..11804)
                     /locus_tag="PKL17_12910"
     CDS             complement(10740..11804)
                     /locus_tag="PKL17_12910"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNK45682.1"
                     /translation="MRSLLRRALPLLVAAFALVLALRGINLRELAEMTRDAPLLSLLG
                     FSALMVLLNCAADTFAMYFVFKWFGMTLRFSELYVIRAATYTLAVINYHAGQLGIIGY
                     IHRVSKVPLSRASAYILFIVGVWVALLLIFAAGSLLLGGAKAAALLPVLVMFFAGIIV
                     YALLLTWPPRFLLSGPPKVTEHESLSVRVWRRLWRVTHKLWEPLHQAGIKGHLRALVV
                     RVPHLLVLLVWHFVALRCFHVDVPVHIAVLYLPVVFAVASLPISVQGLGTSQMAAKYF
                     FSEFSPRGDAAVLAYSLTMTAISTASNLLMGLLFLRAGAKLGLKEMTAEAEQELSEEA
                     AEASAEATSSEESAQVQATP"
     gene            complement(11848..13602)
                     /locus_tag="PKL17_12915"
     CDS             complement(11848..13602)
                     /locus_tag="PKL17_12915"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNK45683.1"
                     /translation="MTEASGTPPLPNRNLGIDLARGLAVVMMIETHALDGWVNAADKL
                     SLGYRFSRVFSNIPAPLFLLLAGLSLGLQAERAERTGKDPAQLRHSMAMRALEVVGYG
                     YLVSLIYAILDFRFALATILRADILHCIGLSLLVCTYFLVGRSAIRGRVLTLCVLGLL
                     LGLCQRFVPPLPLPLASVLGLLVDVAPITRFPLLPLCGFAAIGFWVGHSLRPTEWPVP
                     KCLLWAVVAVALVYPLSMLTHWTLAALGGRLSRAHPAVVWNFLEGTARALSVLSLSLA
                     LAVKLPPSTYGFLLRLGRGSLLAYGFHIPLCYGRVATPILGKLSIGPATVLLVLLIGL
                     TWLVVWGRDTWQASTSPPRGPAGKSAKATALSLIFALLWFVPTHASAQVTNPTQTPAP
                     LSTPNPVPAQVAKRLAAQGRYALAYSLTEAPEYRERIATPTEFFLRAEIDRSHQLFKS
                     GLFRESADAYALLYVMRPDLPQSLFNIGQALRRAGEHEPALTFYRRYLDVEPQTSLRA
                     EVEGYIRELTALLSAHDQLGRKPTPVYKKAWFWLTIGSAVAVTTAVVVGVTIGTRSPA
                     PPPTSEQVLGPFEVVFPR"
     gene            complement(13606..14427)
                     /locus_tag="PKL17_12920"
     CDS             complement(13606..14427)
                     /locus_tag="PKL17_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HNK45684.1"
                     /translation="MFRLRAGTAVCELAPGRGGLITRFAVGSDEVLYLDRRTFSDRKQ
                     NVRGGIPVLFPTAGRLSGDRYSSGGKSYPMRQHGLARHAAWEVVDVQLAKVTMEFRST
                     PMSRVNFPFDFVLRMVVDLGRAGYRTLAIDVTVENTGHEPMPVHFGLHPYFLVSDKDR
                     DRIRVEVDANYAFDNNSGETGPWNRELDLTVPGVDLHLTDLQSQTVLLHLPGKTPRRI
                     EMSDFFTTVVLWQSAMQDFLCVEPWSAPSDALNTGVCLRTIEPGDSIFGEVTISV"
     gene            14580..>16176
                     /locus_tag="PKL17_12925"
     CDS             14580..>16176
                     /locus_tag="PKL17_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45685.1"
                     /translation="MANCAKLRLGLALWFGLGWTAAARGQTPPAASDATASASAPSSA
                     QAQSEPVPSETTAWDLALEGRKLLKAKDIAGAIAKFQAALARADHDELAADDRALIAY
                     LLSLAASNSGNDELSLSAIRDAVKYAPKEADYQLELANQLFANDENLEAKLHVEEALK
                     IGLSSDDDRKDAEALLQKTKSAMLHERLSIDLSVTTGYDSNVIQGGQAETIGGVPTGA
                     RQTTTSTQAFREQLRQRNLDLLKSLLTNYKDAIATNYREAVPSVSEYDIPVTVGLDLG
                     GRLYGNRTTALWTGYRFTQLFMTSPSNDHDSYSLQEHVIPLRLEWRPRSWFLFRPRIE
                     GYANFTGLKTFAPFQGGVTAVLDFLFTESRRWRTRVLGTYQLRQSFDRTYSYLDGNRI
                     DAKVVQELRINQGGSVWARGQLSYRFRADLSGVIEQSVDLQVLSLRGEPITVGSYDYK
                     NPLSYLGNEVGTRWRLYVPKGFDLGIGASLDLRSYRDATTAVYSASPLTINCPATTTA
                     PNCPAGTTVTVPRDGTTTIDLPAT"
BASE COUNT         3220 a         4761 c         5131 g         3064 t
ORIGIN      
        1 atttctgttt tgtgttgatc agttggagcc gtccatgagt acgagtacat ctgaagaaag
       61 actccttctg gagtatctcc agcagcgaca tgggctccgc atggagcaag cggagcaaca
      121 tcatcgagcg tggcaaaacc tggattcaga tggaaaacga gcgccgcgtc catttgctga
      181 ttttctcgtt gaaaaacagc ttctggatcg ggtcactgcg aagaccctga aagccatgcg
      241 gatgggctac ctaagcgctt ccgatgccga aatgcgaagc acgcttggac tcactgcagc
      301 accagaaaca aaagtggccc aggcccccca agtggaaaaa caggagtctc ctgtcgttcc
      361 aaaggaagcg ccggtactac caagggacac gcctggcgcg aaagcggagg tggccgtccc
      421 tgcggcacag agccgatcag cgatccctga tgtcagcatt cccttggttc gatcggatgc
      481 agagattcct gcgatcgaga ttgttgccag tacatcgcag gatacaaagg cactgcgcgc
      541 ggaacatgtg ccggagaatc gtcccagtcc agaccatccg gaaagctccc ggctccaagt
      601 cggtcagtcg ctcggacgct acgatctgga agaagtgtta ggggagggga gtacggcggt
      661 catttaccgc tcctatcaca aactgctgca gatgcccgtt gccatcaaac gattcaaacc
      721 cgctgtgctt tctcgtcgga tgggcagccg cgagcggatt gtgcaggaag gtcgcttgat
      781 ggtccgaatg gatcatccca acatcgttcg ggtgctcgac attgatgaat ccgacggcgt
      841 tccctacatt gttttcgagt atgtcagcga gctaagcctg caggcgttga tcgacaacat
      901 cgggcgtctt tcatctgaac ggcttgccca gattggcgcg caggttgctg ctgcactttc
      961 cgctgcaagc tccgctggac ttctgcaccg tgacgtcaag ccggaaaata tcttggtgcg
     1021 caaagacggt ctgatcaagc ttgccgactt tggcatcgcg acgtatctgc ttcctgatgg
     1081 aaccaccgtc gacgagctgg cgcgcgcggg gctcatcagt ggcactccgc agtacatttc
     1141 accggagcag atatctacgc ctgcacagat cgattttcgg gccgatattt acagtctggg
     1201 ggcgacgctt tatcacgccg cgaccggaca tccgccgttt gatttcagta cgcttgatga
     1261 gctgttgaat gcccatctcg cgatggcgcc gctgccgctg gcttcgttgg ttccgggcat
     1321 tgatgcggaa ctggcgcagc tcatcgcgca gatgatgcaa aaacgacctg aagacagacc
     1381 gcagtctctt accgagctaa gtgagaagtt tgagcgaatc gccgaccgag tcttgcattc
     1441 cgaaagtgtg catcgaaaaa acgtcatcgc cacgcagtcc agctcccatc ctgtgattgc
     1501 agtggacggc gcagcttcga cgcaggtcgg tcgcgcggtc gaaagcactg tggttcatga
     1561 agagaaaccg tcgctgaaga accgaaattc ggctcccttt gtggtggact tgtcagtcct
     1621 ttgggggcgg aacattgcga tcccgctgat cctgcttgtc tgcgtgctgg ggattgcgct
     1681 tgtgctgtgg ttcgctgttg ttcgataggg aaggtgaaaa gacatgcagc gcgtttctgt
     1741 ctcgtcgatt ctgtctgcgc ttcagcagga ctgcgcatca ttcctacgag attcctcgta
     1801 caccccggtg ccgattcctg cgcatttttc tgaaatgaaa ggaacgctcc gcggagaaaa
     1861 catcacgctg aacacgcggc gcttcctggg ccaccactat cggtcgatca cgatggcgtg
     1921 gatcgtgcga gattccgatg gttcggtatg ttcactgacg gtgattggac tcccaacgca
     1981 ggactcgggc gccccgattc tgggtgtcga tttgattggc ttgggcggat cgctctcact
     2041 tgtcgccatc gacctggcac ccaccgatga tgcgacatat tccgaacgaa gcatgccgcg
     2101 tctgcggtca ctccacagca tgacagacga ttcggtggtg catcggaagc gacccgagtt
     2161 ctgccaagat acgttttctc cgctggccct gatctgcgca ggaaaacccg gacacgaagc
     2221 gcagttgcag tcggcatgcg cgacgtttct gtcaatggag ctggacgcca gaaagcaaac
     2281 cagtccgttt gttgtgttgc ctgccgagcg ggccgattct gccgcatcca aacagcgagc
     2341 ttggctgctg agtgaaagga aaaacagaaa agaggcaaaa tatatgtctc ttatgttcgg
     2401 ggaagacctg gctgcggact atctgtacaa cttcctgttt gaggttccca gtgtgtaatc
     2461 catccatagg tgctactgaa ccgctgtctg taaggcttcg gactggtacc cggaaagtgc
     2521 atcgttccgt cgaaaaagtt caattcattc gatcatttct caagggaatg ctgcaccggg
     2581 aaagctactt gcagtatctg cgtgatctgt acgttgtgta ttccgcgctg gaacagcagc
     2641 tttcgtttca caatcggaat ccgaagctgt ctgcgctgtt tcttccggcg ctttttcgat
     2701 cggcagcgct gcggcgcgat ctgatctgtc tcgctggtcc cgactttgag cgccgcattt
     2761 ccccatcccc tgtggcaatc gagtacgcgc gtgcgctcgt ccgcttgtcc catacccagc
     2821 cctacctgct gtctgcacat gcgtacaccc gctaccttgg cgacttgtcg ggtggacagg
     2881 tgcttcacaa gatggcgcag ctagggcttg ggatatccgg gccggaaggt ctttcgtttt
     2941 atcaatttcc acttgtgtcc gacgtgcctg ccatgcgcgt gatgtatcgt cagcagctcg
     3001 atctgttgcc tctttcgggc gatgaaatga cagcgattgt ggacgaagcg tgtcgtgcgt
     3061 ttgcatggaa ccaatcgctg ctcgtgcagc ttcctgtgcc cgcggttcgg ttttcggaaa
     3121 caccgcgctt cgttgcgcaa gcaatacaga atgcagggta aaatgggctt tgcccatcat
     3181 gtcgcctgcg tcagtgccca tcccggataa cgccagccaa gctgccgtcg gtgaaggaac
     3241 cgaactggga cgctatcggc tgatccgaaa gctgggcgaa ggcggcatgg gggtcgtcta
     3301 cgaagccgag catcgcgagc ttggtcgcaa ggttgccatc aaagttctgc gcaccggaac
     3361 cacaagcagc gacattcagg ctgcccgctt ccagcgcgaa gcagagcttg tgtcacgcat
     3421 cggtcatcca aacatcgttg aaatctacga ctttggtcgc acttcagacg gcaatttata
     3481 ctttgtgatg gagcttctgc acggcgagtc tttgcgcagc cggctgcgta ctcgaccgct
     3541 ttctgatcag gaaatcgccg agatttttgc accgctgctg tctgccatcg ctgctgccca
     3601 cagccaagga atcgttcaca gagacctgaa acccgacaac gtgatgttgg gggatgcggg
     3661 ggtgaagctg ctggactttg gggtggcgaa gaatctgagc cagcaaggtg cggacatgac
     3721 gacggtgagt ggggcactgc tgggaacgcc ggcgtatatg gcgccggaac agattcggca
     3781 ttcctcacat gtggatgcac gggcggacat ttatgccgca gcggtgatgc tgtatgaagc
     3841 cctgaccgga caaaggccgt ttgtgggcaa tgcgatagag ctgctgtcaa aacaactgtt
     3901 tgaatcggtg gtgccgccgt cggaagttgc gcgccagcag cgcatcgaca ggcccaacat
     3961 cgactggccg acgatggacc gcattgtgtt gcgagggctc tgcaaggaac cggaaggccg
     4021 ctatcccgaa gtcggtacgt tcctgcatga acttcagacg gcctggccgc gggtggaagt
     4081 tccggatctg tcggggaagc actcgcttgg cgagacagcc gcgagtggga actcgattcc
     4141 ggaagaccat cggtcgtcgt ggcttcagcg gcttcaaatg gtgcggctgg tcgcgctggg
     4201 cgctgtgttg gcgctgctgg gagcgggtct gctgtggcgg cttctgcgga atcccatgcc
     4261 ttcgcagaaa ccgtccatgc aggtgcaggc gcagtcgccc accgattccg aacattcgcc
     4321 ggcagagcgg gcgcagcagc ggattttggc ggcgctggca ggaccgcgtg aagaacggcg
     4381 ggcgctggca ctggcgatgg ccgacgtcgg tggatcggcg ctgcatgacc ggcttttgca
     4441 gcttctgcgt gacccagaac cggcggtggc gaaagcggcg ctgcaagcgg cgggatccta
     4501 tggggtggcg gtggacccgg cgatgattgc cgcactcaaa cagcttggcg aacaagcggg
     4561 tggggcgctt ggcgtcgatg cgatgacggc ccgcttgggg ctcggtgaca cgacggtggt
     4621 ggcgggactc tctgctgcgg tggcgaagcc gtcgctggat gtcgaggtgc ggctgcgggc
     4681 ccagttggcg cttgtgcaag cgggggcgct caagccttcg gcgctgcgcg cggcggttct
     4741 ccagtcggtc gagcaaggcg gtctgcgtcc gacgctgtat cgccgagccc tgcgcgagct
     4801 gttgcggctg aaagatccgg gcacggtgaa gcggctcgac cgcgcgctga ccgatgccaa
     4861 tccgaacctg cgcagtgagg cggcggaagt gctttcgctt ttgggccgtc gcgatggaca
     4921 agatgcgctg taccagttgg cgatgcagca tcccgacgag ctttcgtttc gcacgctgct
     4981 cgcggaatcg ggtgatgtgc gggccaagga acttctgcgg cctcggcttt ctgatccgtc
     5041 gggcatggtg cggcaaaaag cggtcgcggc gctggcggca ctttcggtcc acggcggtcg
     5101 tccctgcgac gtgtctcttg tggtgccgct gctgcgcgat gccgatgcgc tggtgcggct
     5161 gagcgcggcg gtggcgtttg tgcagttgga tcgctgtcaa cgggccgagg cgacggcgac
     5221 gccatgagct ttgatccaca gtcgcttatt ggacaaaccg tcggtgaatt caagctgacg
     5281 gcgcttttgt cgcggaccga ttcggtgatt cgttaccggg ccgaacatca gaagctgcga
     5341 cgggtggcga cgtgccatgt gctggaccca cgcgaaacga gcagccacgg acaggcattt
     5401 ctcgacggag ctcgccgcat aagtcaaatc cgccagcgca acctgatcga catttttgac
     5461 agcggcacca tcttggacag tccgtatttc gtcagcgagg aactgctcgg cgagccgctt
     5521 ttgacgtggt gttcgcgaca tcgcttttcg gtcagtcggc tgttccgaat cgcccagcaa
     5581 gtcgaagccg cgctgctggc cattgaccaa gccggcgctg cggtttctgc gctgtcgccc
     5641 gactcgctgt ttgtgcgccg cgtggcgggt tccctgcaag tcaagctggt cgatgtaacg
     5701 ctcgtgccgg ttcacaaatc gaatgagcaa tcgccgaccc atatcagcat ttccgtcgca
     5761 gccacgtttc tgtttggact tgtccaggcc cagccatttg cgcagccgct gcctgccttg
     5821 ccgctgtcaa ggcgctcgct tttcgacggg gtcgggcgtc tttccggcct gggcgatgat
     5881 ctggaaccgg tgttgcgacg tggactgggg atcgaaacac cgccgttcga ttcgctgcgg
     5941 tcgtttcttt cggcagccct ggcgacgatt cccaagcggg cgccgcttcc gctggaaaat
     6001 gtccgggccg cgctgcgcaa gccgcggact tggctgcttg ccggaccgct gattttgctt
     6061 ttgctggttc cgctggcgct gcttttgcac cgacctcgca ccaagccacc cgtcgctgaa
     6121 aagacgtata cggttcgcga gctgcatgat ctggcccgca ccacacttga agccggactg
     6181 cgcagtccgc tgccgcttgt gcgcgagcag gctctgacgg ggcttttggt cggcgcggac
     6241 attggctggc aggcgcaggt cgagccgctg cttgatgatc cgtcgcccag cgttcaaacc
     6301 cgcgccgccg aagtgctggg cgggctcgga tcgcgacgca cgctgccgac gctggaagcc
     6361 catctgggcc gcgaatacga tccgattgtg cgtgccacat cgtcgcagag catccacaag
     6421 ctgggaaagt cggtcaatcg ggccgcagtc tcgtcgctgc tcaccgggga caacgtgcgt
     6481 gccaagcgaa tcgccgccca gctactggcc gagcaaggtg atgccgaagc cgagcggctt
     6541 gtggcagagc tgcaaggaca gaccggcgag gaagcgcagg aagccaggct gtgggcggct
     6601 cgtcggggtc aagatgcggc gcaagcgctg ctgcggtcgt ctttgcccaa gacgccaccg
     6661 ctcgacgtaa aagacctgcc cgtggcggag ctgtttttgc ggcaagaagt cgcggaatcg
     6721 cgcgagctgc tggtcgcgac ggcaaaacag cccggtcctg cccagacgcg ggcggcgcaa
     6781 attctgtgca aaaacgaaaa ttcgacgttt ctgccgctgc tgcgtgacgt ggtggtcgat
     6841 gcgggccggg cgcttccgga acggctggtg gcagcatcgg ggctggcgcg ctgcggcgag
     6901 cgacgcgacg ggcagctctt ggcgcggctt ttgttggatg gtgcaacaaa cgaacggctg
     6961 aaacagggct gtgccggggc gctgcttgcg ctgaccagtc gtgatccgga cttgtcggcg
     7021 caacagaaca tggcctgggc caagcaggcg ctcgacgatg aaagctggtc ggtgcgagaa
     7081 tcggcggttg cgctgctggg cgaaagtgaa acgcaagaac cggaacgtca ggcggtggcc
     7141 aagcggacgg aaactgcgga aagcgcgaaa ccggcaccga ctccgcaagt tgtacagctt
     7201 ttggtccgtg ccatggacga tgccgcgctg ccggtgcgaa tgaccgcgat gcgttcggcg
     7261 gtgaaagtgg ctcgcaagtc gcaaaagcca agtgagcaag acagcctgaa acaatcgctg
     7321 cgcaagcggg ccgagacggg acttcccgaa gaacaaatcg tcgctgcggc atcgttatta
     7381 cgcattggag atgcttcata tcgggacacg ctgcacaaag gtctgcgcgc ggcagaagtc
     7441 gcggttcgtc gacgggctat cgaagaagcg gacgccgatc ccaagctgcc gagcaagctg
     7501 cttgtgccgc tgacaaacga tcaagacttt gcggtccgat ttggcgcagc ggcgctgttg
     7561 gtcgcgcaag ggcagaaaaa accggcgctt gtgcagacgc tgcgggaagc cgtgcagcgc
     7621 ggcggcagtg aaggtgtccg agcgtatgct ctgctacaaa aattgggtga gctatcgccg
     7681 caaacggaat cgaaagcgcc cgacttggac acgctcttgg ccagctcgaa tgtgctggag
     7741 cggctgatgg cgattgatgc gctgcaaaag ctacccgcgg aagaagccgc accgcttttg
     7801 ctcaaagcga cgcgcgatcc tgattcaacg gtgcgtttcc gtttggtcga tgtcgtcgcg
     7861 catcactgcc agccgggacc gtcgcagcgg gcggcactac cggcgctgcg ggcgctggcc
     7921 gaagaccacg acgtggcggt gcgggcgcgg gcatttgaac tgctttcgca gcttcaggcc
     7981 acgctgtcca aagacggtga tgcagggcca gatgccgcaa aggcggacgg caagaccgat
     8041 tccgccgcag gcaacagcga aaacgcgatg ggcgagtccc ctccggcgct atcccccgag
     8101 gctgcccgca agagccagtc tgttgatcag cagtttgcaa gtggcagcgc gctgttcaag
     8161 gccggcaact atcgcggggc ccaaaaagca ctggaaaaga ccagccagat gtgtacggcg
     8221 taccgtggcg atgtgcccaa gtgtacgcaa attggcagta atctatcata ccaattgggg
     8281 gttacttatg agaacctagg ccagttggct agcgcgatga ccgagtacca aaaggtcttg
     8341 caaggcacag gcggcggcaa agggggcaag cgaccgcgtg gtctggcgat cagcgacgcg
     8401 gcgcagcaag gcgtcgagcg gctgcgcggc aagctcgggc ggcttgtgct gtataagacg
     8461 ttggctggca agtgtcagaa agtcgaagtc tacatgccgc ccggcaagca cctggtcaac
     8521 gtcggtgggg ctgtgcgcaa gccggtggaa ctggatgcgg gcgagacggt cgagcttcgc
     8581 aactgccggt agcgcgtctg ttcgcgccgt ggtgctcatc ctccttgttt tggggatggc
     8641 acagggcgcg tcgggcaaag ggccgcggcg gggcgcggta aaagcgactc cggcggtgct
     8701 tcgcgaaaaa gtccaggctg cacttgcgcg atcggacttt gccgaagccc acaagctggt
     8761 cgacgattcg tatcggcagg cgccgcagcc ggaaaagctg tttctgctgg cccaggtggc
     8821 gcaagcggaa ggccaggcgc aagcggcgcg cgatctgttc cggcggtttc tcgctgacat
     8881 ggggacggaa ggtccgcagt cgcaaaaact ggcgcaatct gccgtcgctg ggccgctgct
     8941 accgagcggt gaagtcaacg tcgaaggcga ccgcggggcg ctggtctacg tcgacgatca
     9001 tcccgtcggg gtgttgccgc ttttgacgcc tttgtggttg tcgacggggg cgcacaaaat
     9061 ttcggtcctg tccggcagca aacggcaaga agaacagtac aaagtcttgg ccgcccgcaa
     9121 agccgacttg cgcttcaact tgtcgtcgga cgtggtggtg gcgacgctgc aaccggcggc
     9181 gctgcttgtg ctgtttccga cggcgagcga aggacaaaag acgctttcgc tttcacccga
     9241 cgaggtgtcg gcgctggaac aaggggcgca gagcgcactt tcgggcaagc cgctcgggct
     9301 gcagacgcaa gcggcagcgc tgctggcggc gccgcatctg gcaaactgtc tggccgattg
     9361 gaactgccaa aagacgctcg gcgaggaaaa ccaggccaac tggctgctct tgctcagcgt
     9421 cgaagtgacg cagcgctggc gaggggcgcg tgatctgaag ctgcgactgg cggcgcacga
     9481 tccggcgatt gccgagcccg cagcgagtgc ggaacgcatg tgcacggcct gtacggcgga
     9541 caaagcaaaa caaatcgcga cggagctgtt cggtgtcgtt gtgccggaag ccaccagtcg
     9601 accgcgcggg acgctgattg tgcagtcggt gcccgacgga gccgaagtgc atctgggcgc
     9661 gcgactgatg ggacacacac cgctgaagcg cacggtctgg gcgggaacat atccgcttgt
     9721 gatcagcaag cgcggttttt cgccgcttca gcgcgaagtg acggtgacag cgggacagcg
     9781 gcagacggtg gaagtgacgc tgcttttggg gacgctcggc gacgcgacga aagtgcctag
     9841 ctcacttctg cccgagtaca gcgaggagcc gtcgaatccg gcgaagactg ccgccgggca
     9901 gcgtccgcgt tggcgcattg tcactggggc atctgccgtg gtgttaggag ccgtgaccgt
     9961 tggttttggc atctcggcac tggcggtcaa caaccactgt gtcgccgggc ttcaggatgc
    10021 gtcgatgcag tgcgggcaga tttacgacac gctgccgcct ggggccgcgc tcacttccgt
    10081 cggtggtgtc gcgcttttgg cgggatcgat gctgctggcc tggccgggat aaagcgcggg
    10141 ccggacccgt tctaggcgcg acgatgcggg cgtgaagcgg acagcacgcg gtcgcgggca
    10201 tcttcttcta tcgcgcgttc gcggcgctcg cgctgacgcc gctgttcccg tcgctcggcg
    10261 gcttcgtgct gctcggctgc ttctcgacgg gccacgcaca gccgcaggtg cgcttgcagt
    10321 tcctcgatca agcgacgcag ctggtttacc tggtggcctc gcgccgcttc atctgcttcc
    10381 gcccgtcgca gttccccgcg cagcagctga agccgcattg cctgttccgc cagttcgttg
    10441 gcagcaatcg tccgcgtgct ctgcgcggtt ttttccgcgg cggcgagcat tttggccatc
    10501 tgctggcgtc tttgtccggc ggcttgttcg ctgtgccgga cccgttctag ctcggcgcgg
    10561 gccgctcgca gctccgcttg ggcttcggct tctgccatcc gtcgcagcga cagcatctgc
    10621 tgcgtaattt gaaggggacg ggcagggcgc gacacccaaa ccatagtacc ggaaaatcgg
    10681 gccgattcgg tctgcaacgc gacggcaagt gcagtcactg caagtgtttt gccgctcgct
    10741 tacggagtcg cttgtacctg tgcgctttct tcgctgctgg tcgcttcggc gcttgcttcg
    10801 gcagcttctt cgctcagctc ttgttcggct tcggcggtca tttctttgag gcccagcttg
    10861 gccccggcgc gcagaaacaa aagccccatc agcaagttgc tcgccgtcga aatcgccgtc
    10921 atcgtcaggc tgtacgccag caccgccgca tcaccccgag gtgaaaactc cgaaaagaaa
    10981 tacttggcgg ccatctgcga cgtaccaagt ccctgcacgc tgataggcag cgatgcgacc
    11041 gcgaacacca cgggcaggta cagcaccgca atgtggaccg gcacatcgac gtggaagcag
    11101 cgcagcgcga caaagtgcca aaccagcagc accaaaagat gtggcacccg cacgacgagc
    11161 gcgcgcaagt gacctttgat tccggcttga tgcagcggct cccacagctt gtgggtcacg
    11221 cgccacagcc gtcgccacac ccgtaccgaa aggctctcgt gttcggtgac tttgggcggt
    11281 cccgacagaa gaaaccgcgg cggccaggtc agcagcagcg cgtagacgat gattccggcg
    11341 aaaaacatga ccagcacggg caaaagtgcc gcggccttgg ccccgccgag taggagactt
    11401 ccggcggcga aaatcagcag cagcgccacc catacaccaa cgataaacag gatgtacgcg
    11461 ctggctcgcg aaagcggcac tttgctgacg cggtggatgt atccgatgat gcctagctgc
    11521 ccggcgtgat agttgatgac ggccagcgtg tacgtcgcgg cgcggatgac gtacagctcc
    11581 gaaaagcgca gcgtcatccc gaaccacttg aagacaaaat acatcgcgaa ggtatcggcg
    11641 gcgcagttga ggagcaccat cagcgccgaa aaaccgagca gcgagagcag cggcgcgtcg
    11701 cgggtcatct ctgccagctc gcgcagattg atgccccgca aggccaagac cagcgcaaag
    11761 gccgccacaa gcagcgggag tgcgcgacga agaagcgaac gcatggcgcg cacgctaaca
    11821 cagcgaaccg gaatcgcgaa aggccagcta gcgcggaaaa accacctcaa acggtccgag
    11881 cacttgttcc gaagtcggcg gcggcgctgg gctgcgcgtt ccgatcgtca ctccgaccac
    11941 cactgccgtc gtcaccgcaa ccgccgaacc aatcgtcagc caaaaccacg cttttttgta
    12001 aaccggcgtc ggcttccgcc caagctgatc gtgcgccgac aaaagcgccg tgagctcgcg
    12061 gatgtagcct tcgacttcgg cgcgcaggct ggtctgcggt tcgacatcca ggtagcgacg
    12121 ataaaacgta agcgccggct cgtgttcgcc cgcccgccgc agcgcttgcc caatgttaaa
    12181 aagcgactgt ggcagatccg gacgcatcac atacagcagc gcgtaggcgt cggcggattc
    12241 gcgaaaaagg ccgctcttga aaagctggtg cgatcggtcg atctcggcgc gcaaaaaaaa
    12301 ctcggtcggc gtcgcaatcc gctcgcggta ttccggcgct tccgtcagcg aataggccag
    12361 cgcgtagcga ccttgcgccg cgagtcgttt ggccacttgc gccggcaccg ggtttggcgt
    12421 cgaaagtggc gccggggttt gggtcgggtt cgttacctgt gcgctcgcgt gcgtcggaac
    12481 aaaccacagc agcgcaaaga tcagtgacaa cgccgtggct tttgccgact tgcccgcagg
    12541 accgcgcggt ggcgatgtgg atgcttgcca ggtatcgcga ccccagacca caagccaggt
    12601 caggccgatc agcagcacca acagaaccgt cgctggcccg atcgacagct tgcccaaaat
    12661 cggtgtcgcc acccgtccgt agcacagcgg aatgtgaaac ccatacgcga gcagcgaacc
    12721 tcggcccagc cgcagcaaaa aaccatacgt cgaaggcggc agcttgaccg ccagcgcaag
    12781 cgacagcgac agcaccgaaa gcgcgcgcgc cgtgccttca aggaagttcc acaccaccgc
    12841 cggatgggcc cgcgacagcc gaccaccgag cgcagccaaa gtccagtgcg tcagcatcga
    12901 aagtggatag acgagcgcga cggccaccac cgcccacagc aggcacttgg gcaccggcca
    12961 ctcggtcgga cgcagcgagt gaccgaccca gaaaccaatc gccgcaaaac cacagagggg
    13021 aagaagcgga aagcgggtga tcggagcgac gtcgacaaga agcccgagca cggacgcaag
    13081 gggcagtggc agcggcggca caaagcgctg acacagtcca agcaaaagac ccagcacgca
    13141 gagcgtcagc acccgtccac gtatcgccga gcgcccgacg agaaaatacg tacagacgag
    13201 cagcgacagg ccaatgcagt gcaaaatgtc ggcccgcaga atcgtcgcca gcgcaaaacg
    13261 aaagtcgagg atcgcgtaga tcaggctgac caaatagcca tagccgacga cttccagagc
    13321 ccgcatcgcc atgctgtgcc gaagctgcgc cggatctttg ccggttcgct cggcgcgttc
    13381 tgcctgcaag cccagcgaca gccccgcgag cagcagaaac agcggcgccg ggatgttcga
    13441 aaagacgcgc gaaaagcgat agcccaggct cagcttgtca gcggcgttca cccagccgtc
    13501 gagcgcgtgg gtttcgatca tcatgaccac cgcaagaccg cgcgccagat cgatccccag
    13561 gttgcggttt ggcaaaggtg gtgtgcctga cgcctccgtc atgggttaca cgctgatcgt
    13621 aacctcgccg aagatggaat cgcccggttc gatcgtgcgc agacaaacgc cggtgttgag
    13681 cgcatcgctt ggtgcgctcc atggctcgac gcacaggaag tcctgcatgg cgctttgcca
    13741 cagcaccacc gtcgtaaaaa aatccgacat ctcgatccgt cgcggcgttt tccccggcag
    13801 atgcaaaagc accgtctgtg actgcaaatc ggtcaagtgc agatcgacgc cgggcacggt
    13861 cagatcgagt tcgcgattcc acggtccggt ttcgcccgag ttgttgtcaa aggcgtagtt
    13921 tgcatcgacc tcgacgcgga tgcggtcgcg atctttgtcg gacaccagga agtacggatg
    13981 caggccgaag tgcaccggca tcggttcatg cccggtattt tcgacggtga catcgatggc
    14041 aagcgtccga taaccggcgc ggcccaggtc gaccaccatc cgcagcacaa agtcgaacgg
    14101 aaaatttacg cggctcatcg gtgtgctgcg aaactccatc gtgactttgg caagctgcac
    14161 gtcgacgact tcccacgcgg catgtcgtgc caggccgtgc tgtcgcatcg gatagctctt
    14221 gccgccgctg gaatagcgat ctccgctgag ccgcccagcg gtcggaaaca gcaccggaat
    14281 gccaccacgg acattctgct tgcggtcaga aaaggtgcgc cgatcgaggt aaagcacctc
    14341 atcgctgccc acggcaaagc gggtgatgag accgccgcga ccgggggcca gctcgcagac
    14401 ggcagttcca gcgcgaagac gaaacatgtc gagccctgac gcatcgcgaa agtgctcggc
    14461 ggttggctgc ttggacgacc catcgaacga catcgccgtg agtgtaacag gcagacgcgc
    14521 agagccaaat gcgtctttgc atgtccccac ggcgcccctt ggcttgctat tctgcgtcta
    14581 tggcaaactg cgcgaagctc cgcttggggc ttgccctttg gtttggtttg ggatggacgg
    14641 ctgctgcccg tggccaaacc cctccggcgg cttcggatgc gacggccagt gcgagcgcac
    14701 cgagcagcgc ccaagctcaa agtgagcccg tcccaagtga aaccacggcg tgggacttgg
    14761 cgctcgaagg tcgcaagctg ctcaaagcca aagacatcgc tggtgcgatt gccaagtttc
    14821 aagcagcgct ggcgagagcc gaccacgatg agctggcagc cgacgatcgc gcgctgattg
    14881 cttacctttt gtcgctggcc gcgagcaaca gcggcaatga tgagctttcg ctgtcggcga
    14941 ttcgcgacgc ggtaaagtac gctcccaaag aagccgatta tcagctggag cttgccaatc
    15001 agctctttgc aaatgatgaa aacctggaag ccaagctgca cgtcgaagaa gcgctgaaga
    15061 tcggcttgtc gtcggatgac gatcgaaaag atgccgaagc gctgctgcaa aaaaccaaat
    15121 cggcgatgct tcatgagcgg ctgtccatcg acctcagcgt cacgacgggt tatgactcga
    15181 acgtcattca aggcggacag gccgaaacca tcggtggtgt tccgacggga gcgcgccaga
    15241 ccacgacttc gacgcaggcc tttcgcgaac agcttcgcca gcgcaacctg gatctcttaa
    15301 aaagtctgct caccaactac aaagacgcga ttgcaacaaa ctaccgagaa gcggttccgt
    15361 cggtcagcga gtatgacatt ccggtcacgg tcgggctcga tctcgggggg cggctgtatg
    15421 ggaatcgcac caccgcactg tggacgggat accgctttac gcagcttttt atgacgtcgc
    15481 cttcgaatga ccacgactcc tattcgctgc aagaacatgt gattccgctg cgactggagt
    15541 ggcgaccacg gagctggttt ctgttccggc ctcgcatcga aggatacgcc aacttcaccg
    15601 ggctgaagac gtttgcgccg tttcagggtg gtgtcaccgc ggtgctcgac tttctgttca
    15661 ctgaatcccg tcgctggaga acgcgggttt tgggcaccta tcagctgcgg cagtcgttcg
    15721 atcggacgta ttcctatctg gatgggaatc gcatcgatgc caaggtggtt caggaactgc
    15781 ggatcaatca gggcggtagt gtgtgggcac gcgggcagct ttcgtatcga ttccgcgccg
    15841 acctaagtgg tgtgatcgaa cagagcgtcg atttgcaggt cctaagcctt cgcggtgagc
    15901 caattaccgt cggaagctac gactacaaaa acccgctttc ctatctgggc aatgaagtag
    15961 ggacgcgctg gcggctgtac gttcccaaag gattcgatct gggaatcggt gccagcttgg
    16021 acctgcgcag ctatcgggat gccaccaccg ccgtctacag cgccagtccc ctgacgatca
    16081 actgtcccgc aaccaccacc gcgccaaact gtccggcagg aacgacggtg acggtgcctc
    16141 gcgatggaac caccacgatc gatttgccgg caacgg
//
LOCUS       DAORGN010000111        16170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1479_length_16170_cov_6.579708, whole genome shotgun sequence.
ACCESSION   DAORGN010000111 DAORGN010000000
VERSION     DAORGN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1479_length_16170_cov_6.579708"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKL17_12930"
     CDS             <1..324
                     /locus_tag="PKL17_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45686.1"
                     /translation="GPTVVAPPPPAPVYRPAPVYAPPTVVAPPPARPYGAPVYTPPPA
                     AAPGYRPPAPAPVPGGGYHAPAPAPTGGYRPPAPAPAPGHAPGTPPPFGGGGPTHRVP
                     APGGM"
     gene            382..1218
                     /locus_tag="PKL17_12935"
     CDS             382..1218
                     /locus_tag="PKL17_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45687.1"
                     /translation="MLPRYLFADVDDTFTVRGGIHPEVLDAVASAERAGIEVILNTGR
                     PAGYGATLLAYLPMVSAVIVENGGAWLDRHTPAPSLHTADGSRGSDHEHGLHFFRPLD
                     SDLRMKLGDLQARVARRLGRTFCTTADNQYRVSDYTVVRDLPGGVEGAALLAEIARLV
                     DEESNQQGQMLASSIHIHFMLDGAERRSKASGAEHLLARRGIRSPAQELAEAAVAVGD
                     SANDASLFAPGRFALSVGVRNIERYLPELGASVPKHITTHHEGLGLCELIGDLLGGRI
                     GV"
     gene            complement(1215..2057)
                     /locus_tag="PKL17_12940"
     CDS             complement(1215..2057)
                     /locus_tag="PKL17_12940"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNK45688.1"
                     /translation="MAETGPLRTIFIVSDSTGETAEKMVRAALRQFDLHEAVTDLRLI
                     PHLRHELDMDVVVGNAQRTQGLVVSTLVRGEERKVLQTKCEQRGVPYVDLIGSLLGTM
                     ARFLSVEPREVPGLLHTVSDSYFRRMEAIEFTVQSDDGRCPENLIKADLVLLGISRTS
                     KTPLSIYLAQKGYRVANVPLVLDLPLPPELDQVPPERVYALTIQPDALLQIRRERLLR
                     IHMATDTSYGQRDYILREIYYARDVLRSHRDWTTIDVTGKAIEETAADVLRLFHQRTG
                     TPMH"
     gene            complement(2064..3365)
                     /locus_tag="PKL17_12945"
     CDS             complement(2064..3365)
                     /locus_tag="PKL17_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNK45689.1"
                     /translation="MFAAWKRWRSRRKLPAPRPFRYERFGGIVQLGGRFGWPQALVFV
                     DRARAVSLGYDAPPAGLWDGDEPPLDDGSPLSAPLEAHLQLTNRCEAGCATCYTGASP
                     QGSPHEWGLAEWCAAIDELSAAGVFHVALGGGESAHLPWLGELLAHCWQRGVIPNLTT
                     SGLYAADVRKRVCDLARRGLFGQINVSFDGVGKTYELVRGFDGFATADETLRALLRVF
                     PDVGINTVLTRVGFPQLAELFDHAKRRGASEIELLRLKPAGRGASPKTYEALRCTDEQ
                     HRGLVETVLSLTRTYGLRTRLDCSLVPFVAHKQFDAKLLRFLSIYGCAGGDLLTAARA
                     DGRLSACSFAVPTAEKVTDLRRHFADPAAFPPFRAYLQADEPCRSCHYLSLCRGGCRV
                     VSLHSEGSLRAPDPECPRVIDWRTQHPAETAPAPRRLKVLR"
     gene            complement(3383..4879)
                     /locus_tag="PKL17_12950"
     CDS             complement(3383..4879)
                     /locus_tag="PKL17_12950"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNK45690.1"
                     /translation="MAQAVGADGASAQEVRGRAPVLGFRRQKPKDSAQSQRGPSCILF
                     LLLLLLSFENTASAEVRGDWVVSQINQRNANVPPPLRAEKYGTMSESIFAFFRATNHL
                     YWSDYGSSPLLATFGGPTTRVWLQGDAHCDNISALLTNQNAVVFDLDDFDDTLIADYQ
                     LDLWRMAVSILLAVQENGGLPQKTQEQLIDALSTSYLNTLRSYQDNASELITTYSVAN
                     TPSFLSDWMAQLSELTHLRVLKKMTTVASGKRMFDFDKPGVQSVAPSVIQALQAAMPT
                     YQATLTGRLRQIPSYFRIKDVAQKTRGGMGSLGVTRYFVLIEGVSASQDDDRILDIKA
                     QSQPAGWPYMDPGVRDQLLTMISGDQAMRVVLGYRAMGYRADEHLGTLSALGERFFVR
                     ERTPVRNSFELTNLVNPQRALPLVQQWGAVLATSHARADRDSSPIISYDFEKELLTKV
                     ATEKQRLAFLARVRQVAFFYAKQVSDDYKAFKEWHAQNAARTQVAGKE"
     gene            5099..5830
                     /locus_tag="PKL17_12955"
     CDS             5099..5830
                     /locus_tag="PKL17_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45691.1"
                     /translation="MTLRLLSLFAQLSLVVVAAGCGEAIDNFKNTDHQGAFILGKVRG
                     IAFNIPLRSASVCLLDEMDNPKSCTSSDAGGEFVFSELPEASQQRVRLENSGYWPLIG
                     MINTKRSVDGSPVWEPAMMDNFVVDLQAKKVEDNFEPDTGLLYFEIQEPDGEEWRGAA
                     NRELRSEQLGSPYYFNDSNWLDKNRKSTASKGSAIFVNVKPGWYPIELPSGCLPHWGF
                     DLDEQGRIPTYVAANAVTELNVRCR"
     gene            complement(5873..7264)
                     /locus_tag="PKL17_12960"
     CDS             complement(5873..7264)
                     /locus_tag="PKL17_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45692.1"
                     /translation="MSRARNVVPGQDKQVSEKTSESAFRGFRRFFASASLRRVCAFAV
                     ALSPACDSLWFSYLEPCDSSRTACQDSLGDRILPSNGIDPAWFGDAKAALEPTQDLVI
                     DTDLGTVVTLAGNQQLADVSYHQVNAADCGKSWKVGIGVFSFKRIRIPEGVRVRFAGT
                     RAAALLSPGRIEIEGLLDLRGSQAECSDMRCGGPGGFPGGYAQNPGVPGDGPGGGLFG
                     HGVGGDGDEAGGGGAGSCGTGGRGGDGNTPALYPGGAGGQPYQSANLIPLCGGSGGGP
                     GGLGKQTTDLTQRGGGGGGAIQIVSKSAVTIGSSAATPSGIQAGGGGGQSDHGTNFND
                     GGGGGGAGGAILIEAPIITISSGAVLAANGGSGGGGYNNGKDTANGVPADLSSTPVLG
                     GLGEVRSGGSGGAGSAQNGSDATGAPGDGGGGGGGAVGRIRLNTQDGTATISGVLSPN
                     MGNCTTIGMLTLR"
     gene            complement(7430..8998)
                     /locus_tag="PKL17_12965"
     CDS             complement(7430..8998)
                     /locus_tag="PKL17_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNK45693.1"
                     /translation="MYRKITLCALSLLVLFCDRAHAQTESPASAQVRPSTDIKQTHTT
                     DPPRVDLKPPLSIEERLIKLEDELRRLREEKAATQTGSGSQPSDFDRPVEPFSWGDFT
                     WINGGSRQTSRLLDSKYFTPQIDIDSNYTYSLNQPIDNTIVGSTATARHNELSLAFAG
                     IGGDVHVGNVHARIFTQFGTRSSAVPRNDLTVNRGQFDLLSVYRYLSEAYAGYHFDKL
                     YGINVDVGLFFSYVGLFSYTQFENWGYQASFTSDNTPWFFNGARLQIFPTERLKVELW
                     LINGWQTYGKFNQLPGVGYQLHYAPREWIKMVSNGYVGTDTQGSASRVRFHSDSSILL
                     RYLNRSESKGISRAAVSFTFDLGFEEGDGVTAFRGVHDPGNCQKERPCEQDFLSAMAY
                     HNLWFWKNRFGFLLGGGFIRNPGRYLVLVPTGVAAATFDTRPGTEFFGWDVSSNLSVY
                     PSDNLTVRLEVSHHEASVPYYAGPGGVTGPDGYKCGGLYSPDGTISTCAPSGWTPDLA
                     NAETKLILALLFRL"
     gene            complement(9166..10713)
                     /locus_tag="PKL17_12970"
     CDS             complement(9166..10713)
                     /locus_tag="PKL17_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK45694.1"
                     /translation="MSGSIKTYRDLDAGAVDSCDVCIIGTGCGGATLGVRLAEAGKQV
                     IFIERGGSYTRQDFDQREDDMLAKIDGGRGLDTSEDMSVQLTYGNNVGGASVHYWADS
                     YRTPRDRIALWTEKYGIEGHGEADLTPHFETLERDLHVHEAEPQFRNRMNQLLEEGAR
                     KLGWSGAPVPIARLPCAKSGYCMQGCSYDFKQSQLVTHIPRALAANARIYADCEAIEL
                     SWNGNRAQSLLARVLHRGEGRPTGAMVRIHAKIFVVAAGGYGTPVFLLRNGLKAKLPA
                     VGEHTYCNPCPMVHALFDEEIIQFRNIPAAFGIDQFRLATFAGDRYIEGGYLLMANQL
                     QPATLAATLPGFGDSHRHLMKNLRKLGGTICWIDDVEEGNIRLDGDQPKYRVPLHGGN
                     GLRIRDAFAKQAKLLLAVGAKELIFGDVHDTRITKESELERAVAKLDITPGKFVLAAP
                     HPAGGARMGKDPKTSVVGMDHRVHGTDNLYVADPSVFPTAPSVDPSLTIMAYSCIAAE
                     KIRAVLG"
     gene            complement(10710..11294)
                     /locus_tag="PKL17_12975"
     CDS             complement(10710..11294)
                     /locus_tag="PKL17_12975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HNK45695.1"
                     /translation="MSLPPNRRLFLQIGIGSAALLAVSFGLLRHFQHGYAEQLSPSDI
                     PLALSTKAFAIVKAIVRTLTPAGDGFPDGVELGIPQRIDEEFYVAPPAIRSALAAGLQ
                     LLEHATLVHGYSHRFTALAPDQQRTYLQTLSTGKQEALRQVVLAIKQVVHLFYYAHPS
                     VWGHIGYDGPFVKEPVYPDSHVLYQDLLRKRRAS"
     gene            11663..13696
                     /locus_tag="PKL17_12980"
     CDS             11663..13696
                     /locus_tag="PKL17_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNK45696.1"
                     /translation="MQDSTSQAIPAAFPPQSVLLRYAPTALSQGCWLWQSVQVKQTHT
                     ALGGALVDAFFLMCGEGDFAQHPASQYRGLLSSVSVCLPPLSSRAFVEDPRLCDADFA
                     LPLFGLFVGNQPASALPDVLGIHAAMMLWGPPPAVCQAMKNCASASQLQSWEQNTDRQ
                     ERALALIRKTLTLYAEAGNPNWPRVLAVAAKLRDLRSAFVDALVPSAPPTAWENMLAL
                     VQRKFRHGFGFHRHVHLAGRSLDRFFDPQKPDLQGFLIALSRSHFVTPGRPEQSLILR
                     RTTQFGGTMFGIFDDQELAVLSSWIAALPDSAVPEIATPQATQDHGLDERDNEGTATK
                     TETAWETLGGSPFGGTGPLPSLDLPSLYHHLLADAGDPAWTACAKTYLAERITEVAKA
                     GSVDHLTKQKLWPYSASRLADWVDAQLRQQVLGNPDVGGKTEPQEDMGIERHLHKSEV
                     LWLLTQLAPAALIDGAWLQGITTPLFSAQIVTPLLFQIYRDELGAGVPQQHHGNLMRS
                     VLVAEGIVLPDCDSLDFVRYPGFLPASFSTPVLWLAISRCTDSFFPELLGLNLAIELA
                     GVGKGYDRATALLRKHGIDPYFFVLHNTIDNGASGHTAWSVRAIVLYLEQLCATADQE
                     AVATCWQRIFRGYALYAKSSLPLLSAIGMRMGPRLGMRWLWRFLTGKTAAESS"
     gene            13699..15678
                     /gene="asnB"
                     /locus_tag="PKL17_12985"
     CDS             13699..15678
                     /gene="asnB"
                     /locus_tag="PKL17_12985"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNK45697.1"
                     /translation="MCGIVGAVDLQGRRNFDPQRLADMAASVRHRGPDDAFAYLAPGI
                     AMATQRLAIQDVAAGRQPLTDPRGQIVVSQNGELLNADSLRSELARQGHSFRSHCDTE
                     VWPALYLSAKTDAFVRAQGQFAVALWDRPDRTLYLARDRVGICPLYYTVAQGYLLWAS
                     EIKALLASGLVNPEVDREGIDLVFTLFAAGTKRTAFRNICSLWPGHYLRVRSGDIATI
                     RYWDLDFPPQGEERKDRTERLTDELTQIVDSAVSRRFASDGPVATYLSGGLDSSLVLA
                     TASRLRPLQPLHSFTVGFDGAGPDERPQTQQTAALLSAKLHTLVPSARDILDALPSVV
                     LAAEGPFMDTANACLLLLAKQVAAAGFKVVLTGEGADEAMGGYVWHKTAKLFRWLSKL
                     HPGLPSWIRRSVAEYAMQGTPQATWGNRFGNPQPSLFDVYEPLSRARSLLYSDAMTAA
                     VSAHDPFADLDISVDHMRAWHPLNQSLYLEYKLMLPGHLLLGKGDRVAMQSGVEARYP
                     FLDESFVAFCSQLAPEYKLCGFTEKWLLRRVAQRVLPANMRQPQKSMFKANSLCLLKP
                     QPAWIDQLLLPESLRKTGYFSVEKVAHERALQAQLPDFHPRRYLVDGSFTAVVMTQLW
                     HHLFLGGGLCELAPFRVSESSPLPFFSDAGNEHAN"
     gene            15668..>16170
                     /locus_tag="PKL17_12990"
     CDS             15668..>16170
                     /locus_tag="PKL17_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45698.1"
                     /translation="MQTERIGHEGRLLDLVSRRWDKLDWRTPIPVDRLVITESHYMDV
                     ESLGFDPRGRNAINRLLACFTCELFIHFERYVIEFLEHRRHEIPFVPAAALDRFVAEE
                     KAHSDAFYRLLVKLRPDLYSTKATTTTGLRFMHWTLGDDAAIALAPVGSFFLLAWLFE
                     EITIYVPV"
BASE COUNT         3132 a         4794 c         4883 g         3361 t
ORIGIN      
        1 ggaccgacgg tggtagcgcc gccgccgccc gcgccggtgt ataggcccgc gcccgtctac
       61 gcgccgccca cggtggtagc gccgccgccc gcccggcctt atggcgcacc cgtgtacacg
      121 ccgccgccgg ctgccgcccc tggataccgg ccacccgcgc ccgcgcccgt tcccggtggt
      181 gggtatcatg cgcccgcgcc agccccgacg ggtggatacc gtccgcccgc gcccgcgccg
      241 gcccctggtc acgcaccggg tacgccgccg ccgttcggtg gtggaggccc gacccatcgc
      301 gttccggccc ccggtgggat gtagtccggc agctcgtcga aacccccgtc tttttcgccg
      361 cctctgttac agtgcgcggc catgcttcct cgctatttgt ttgctgacgt cgatgacacc
      421 ttcacggtgc gcggcggcat tcaccccgaa gtgctagatg cggtggcttc tgcggaacgc
      481 gcagggatcg aagtgatcct caacaccggg cgtccggcgg gttatggggc gacgcttttg
      541 gcatatctgc cgatggtgag cgcggtgatt gtcgaaaatg gcggggcgtg gcttgaccgt
      601 cacacgccgg ctccttcgct gcacactgcg gacggcagcc gcggatctga tcacgagcac
      661 ggcctgcact tctttcggcc tctggatagc gatctgcgga tgaagcttgg ggatctacag
      721 gcgcgggtag ccaggcggct cgggcggacg ttttgcacga cggcggacaa ccagtaccgg
      781 gtcagcgatt acaccgtggt gcgcgatctg ccgggcgggg tagagggagc ggcgctgctc
      841 gcagagattg cgcgtcttgt cgatgaagag agcaaccagc aaggccagat gttggccagc
      901 tcgatccaca ttcacttcat gctggacggg gccgagcgac gcagcaaagc ttctggcgcc
      961 gaacacctgc tggcccgtcg gggcatccgc tcccctgcgc aagagctggc agaagcggcg
     1021 gtcgcggtcg gtgattcggc caacgatgcg agcttgtttg cgcccggtcg gtttgcgctg
     1081 tccgttggtg ttcgcaacat cgaacgctat cttcccgagc ttggcgcgtc ggttcccaag
     1141 cacatcacga cgcatcacga agggctcggg ctgtgtgagc ttatcggcga tctgctcggc
     1201 ggtcgaatcg gcgtctagtg catcggggtg ccggtgcgct ggtgaaaaag tcgcagcacg
     1261 tcggcggcgg tttcttcgat cgctttgccg gtgacatcga tggtggtcca gtcacgatgg
     1321 ctgcggagca cgtcgcgggc gtaataaatt tcgcgcagga tgtagtcgcg ctggccgtag
     1381 cttgtgtcgg tcgccatgtg gatgcgcaga agtcgttccc ggcggatttg caaaagcgca
     1441 tcgggctgaa tcgtcagcgc atagactcgc tcgggcggga cttggtctag ctcgggcggc
     1501 agcggcaaat cgagcacgag cggcacattt gcgacgcgat aacccttttg cgccaaataa
     1561 atcgacagcg gcgttttcga tgtccggctg atccccagga gcaccaagtc ggccttgatc
     1621 agattttccg ggcagcgtcc atcgtcgctc tggaccgtaa actcgatcgc ttccatgcgg
     1681 cggaaatagc tgtcggacac cgtgtggagc agccccggca cttcgcgtgg ctcgacggac
     1741 agaaagcgcg ccatggtgcc cagaagcgat ccaatcagat cgacataggg gacgccgcgc
     1801 tgctcgcact tggtttgcag cactttgcgt tcttcaccgc gaaccagcgt cgacaccacc
     1861 agaccctggg tgcgctgggc attgccgacg accacgtcca tgtcgagctc gtggcgcagg
     1921 tgcgggatca gccgcaggtc ggtgacagcc tcgtgcagat caaactggcg cagcgccgcc
     1981 cgcaccatct tttcggcagt ctctccggtg gaatcagaga cgataaagat cgtgcgcagc
     2041 ggacccgttt cagccatgtc gcgctaccgt aacactttta gccgacgcgg cgcaggcgct
     2101 gtttcggcgg gatgctgcgt gcgccagtcg atgacgcgcg gacattcggg atcgggggcg
     2161 cgcaggctgc cttcgctgtg cagcgaaacg acccgacagc cgccgcggca cagcgaaaga
     2221 tagtggcaag agcggcacgg ctcgtcggct tgcaagtacg cgcggaaggg cggaaaggcc
     2281 gccggatcgg cgaaatggcg tcgcaggtcg gtcacttttt ccgccgtcgg tacagcaaag
     2341 ctacacgccg acagcctgcc atctgcacgc gctgccgtca gcaaatcacc gccggcacag
     2401 ccatagatgg acagaaagcg caaaagcttg gcgtcaaact gcttatgcgc gacaaacgga
     2461 accagcgaac agtcgagccg ggtgcgcagt ccatacgtcc ttgtcaggga caaaacggtt
     2521 tcgaccaagc ctctgtgttg ttcgtcggtg cagcgcaggg cttcgtaggt tttgggactg
     2581 gcgccgcgtc ccgctggctt gagccgcaga agctcgattt ccgacgcgcc acgtcgcttg
     2641 gcatggtcaa acagctcggc gagctgcgga aaaccgacgc gcgtgagcac ggtgttgatg
     2701 ccgacatcgg gaaacacccg caagagcgcc cgcagcgttt catcggcagt cgcaaagcca
     2761 tcgaagccgc gcacaagttc ataggttttg ccgacgccat cgaacgagac gttgatctgt
     2821 ccaaacagtc cgcgccgcgc cagatcacag acgcgcttgc gcacatcggc tgcgtacagg
     2881 ccgctcgtcg tcaggttggg aatgacgccg cgctgccagc agtgcgcaag cagctcgcca
     2941 agccacggaa ggtgcgcgct ttcgcccccg ccaagcgcga cgtgaaacac gccagcggca
     3001 gagagctcgt cgatggccgc gcaccactcg gcaagacccc attcatgcgg actgccttgc
     3061 ggcgatgcgc cggtataaca agtcgcgcag ccggcttcgc agcggttggt caattgcagg
     3121 tgggcttcga gcggcgcaga cagcggcgat ccatcgtcaa gcggcggctc atccccgtcc
     3181 caaagtccag ccggcggcgc gtcatagcca agcgatacgg cgcgggcgcg atcgacaaac
     3241 accagcgctt gcggccagcc aaaacgaccg ccaagctgca cgatgccgcc gaagcgttca
     3301 tagcggaacg gacgcggcgc ggggagcttt ctgcgactgc gccatctttt ccacgcggca
     3361 aacaccgcag cacaatagcc gactattcct tgcctgcgac ctgcgtgcgc gctgcgtttt
     3421 gcgcatgcca ctctttgaag gctttgtagt cgtcgctgac ttgcttggca taaaagaacg
     3481 cgacctgccg cacccgcgca aggaacgcga gccgctgctt ttccgtcgcg actttcgtca
     3541 gcagttcttt ttcaaagtcg taggaaatga tcggacttga atctctgtcg gcgcgcgcat
     3601 gcgacgtcgc cagcaccgca ccccactgct gcacaagcgg cagcgcgcgc tgcggattga
     3661 ccaagttggt cagctcaaac gagtttcgga ccggcgtccg ttcgcgcaca aaaaagcgct
     3721 cgccgagcgc cgacagcgtt cccagatgct catcggcgcg atagcccatc gctcgataac
     3781 cgagcaccac ccgcatcgct tggtcgcctg aaatcatcgt cagcagttga tcgcgcacgc
     3841 cagggtccat atacggccag cccgccggct gcgactgcgc tttgatatcc aggatgcgat
     3901 cgtcgtcttg gctcgcggac acgccttcga tcagcacgaa atagcgcgtc acacccagcg
     3961 agcccatgcc gccgcgggtc ttttgcgcca cgtctttgat ccgaaaatac gacggaatct
     4021 gccgcagccg tccggtcagc gtcgcttgat acgtcggcat ggcggcttgc agcgcttgaa
     4081 tcaccgacgg tgccaccgac tgtacgcccg gcttgtcgaa atcaaacatt cgcttgcccg
     4141 acgccaccgt ggtcattttt tttagcaccc gcaggtgggt cagctccgac agttgtgcca
     4201 tccagtcgga cagaaagctg ggcgtattgg cgacggaata cgtggtgata agttcgctgg
     4261 cgttgtcctg ataacttcgc agcgtgttta agtagctcgt cgacagcgcg tcaatcagct
     4321 gctcttgtgt cttttgcggc agcccgccgt tttcctgcac cgcgagcaaa atgctgaccg
     4381 ccatccgcca cagatcaagc tggtagtcgg cgatcagcgt gtcgtcgaag tcatcgagat
     4441 cgaagaccac cgcattttgg tttgtcaaaa gcgcgctgat gttgtcgcag tgcgcatcgc
     4501 cttgcagcca gacgcgcgtc gtcggtccgc caaacgtcgc cagcagcggg gaacttccgt
     4561 agtcgctcca gtacagatga ttggtggcgc ggaaaaatgc gaagatagat tccgacatcg
     4621 ttccgtattt ttcggcgcgc agcggcggcg gaacattggc gttgcgctgg ttgatttggc
     4681 tcaccaccca gtcgccccgg acttcggcgg aagcggtgtt ttcaaacgac aagagcagaa
     4741 gcaaaagaaa caggatgcag cttggcccac gctgggactg cgcgctgtct ttcggctttt
     4801 gtcgtcggaa accaagcact ggtgcacgcc cccggacttc ctgtgcactc gccccgtctg
     4861 cgcccacggc ttgtgccatc gtgcgcaaac tcttcttatc gttacaggta tggggggaat
     4921 tggaaaggac tcctttgccg gtcggtcgcc gccgtttcgt cgacagaaag cgggtggtgt
     4981 gtctgtccac gttgtcgccg atgtacgccg cgattttttg cagcgttttg cgcgatcact
     5041 tgggcgaaaa aagcatgcct tgacacacag gtagcggctg atagtacggt gcgcggtcat
     5101 gacgctgcgg cttctgtctt tgtttgccca attgagtctg gttgtcgtcg ctgctggctg
     5161 tggagaagcc atcgacaact tcaaaaacac cgaccaccaa ggcgcgttca tcttgggcaa
     5221 agtgcgcggc atcgcgttta acattccgct gcgcagcgca agcgtctgtc tgctggatga
     5281 aatggacaac cccaagtcgt gtacgtcgag cgatgcgggg ggcgagtttg tcttttcgga
     5341 acttccggaa gccagccagc agcgcgttcg tttggaaaac agtgggtact ggccgctcat
     5401 cggcatgatc aacaccaagc ggagcgtcga tgggagtccg gtctgggaac ccgcgatgat
     5461 ggacaacttt gtcgtcgatt tgcaggccaa aaaagtcgaa gacaactttg aaccggacac
     5521 cggtctgctc tatttcgaga tacaggaacc cgacggtgag gaatggagag gagccgccaa
     5581 tcgcgagctc cgtagtgagc agcttgggag tccttactac tttaacgaca gcaactggct
     5641 cgacaaaaat cgcaagagca ccgcgagcaa aggatcggcg attttcgtga atgtgaagcc
     5701 gggctggtat ccgatagaac ttcccagcgg atgtttgccg cactggggtt ttgatctcga
     5761 cgagcagggc cggattccca cctatgtcgc ggcgaatgcg gtgacggaac tgaacgtgcg
     5821 ctgcagatag tccgcgggat tggggatctg gtgcgccgca cgccgttgtg agtcagcgca
     5881 gggtcaacat tccgattgtg gtgcagttgc ccatattggg actcagtaca ccgctgatcg
     5941 tcgcggttcc gtcctgggtg ttcagtcgaa tccgacccac agcgccgccg ccgccaccgc
     6001 cgccatcacc tggggctccg gtcgcatcgc tgccattttg tgcagagcca gcgccgcctg
     6061 atccgccgga tcgtacttct cccaaaccgc caagtaccgg cgtcgacgaa agatcggcgg
     6121 gcacaccgtt ggcggtgtct ttcccgttgt tgtaaccgcc gccgccgctg ccaccgttgg
     6181 ctgccagtac ggcaccgctc gatatggtga tgatgggtgc ttctatcagg atcgcaccgc
     6241 ccgcgccacc gccgccaccg ccgtcgttga agttggtgcc gtggtcgctt tggccgccgc
     6301 cgccgccggc ctgaatgcca cttggcgtcg ccgcagagga gccaatcgtc accgccgact
     6361 tcgacacgat ctgaatcgcg ccgccgccgc caccgccacg ctgggtgaga tcggtcgtct
     6421 gcttgcccag accgccggga ccaccgcctg atccaccaca gagcgggata aggtttgcac
     6481 tttggtacgg ctggccgccc gctccacctg gatagagcgc cggggtgttg ccgtcgccgc
     6541 ctctgccgcc ggtgccacag gaacctgcac caccgccgcc ggcttcgtcg ccatcaccac
     6601 cgacaccatg accgaagagt cccccaccgg ggccatcgcc gggcacaccg ggattttgcg
     6661 cataaccgcc agggaaacca ccgggaccgc cgcagcgcat gtcactgcat tcggcctgac
     6721 tgccgcgcaa gtcgagcaga ccctcgattt caatgcggcc aggggacaaa agtgcggcag
     6781 cgcgggtgcc ggcaaaacgg acgcgaacgc cttcggggat gcggattcgc ttaaaagaaa
     6841 acacgccgat gccgactttc cagctcttgc cgcagtccgc cgcattgact tggtggtagc
     6901 tgacgtcggc gagctgctga ttgccggcca gcgtgacgac cgtgcccaga tcggtgtcga
     6961 tcaccagatc ctgcgtcggt tcgagcgccg ccttggcatc gccaaaccag gctggatcga
     7021 tgccgtttga gggaaggatg cgatctccta aagaatcctg gcaggccgtg cgcgacgagt
     7081 cacagggttc caaataggaa aaccacagcg aatcgcatgc cggggaaagc gcgaccgcaa
     7141 acgcacagac ccgacgcagc gaggcggaag caaaaaaacg acggaagccg cgaaacgccg
     7201 attcggaggt cttttccgaa acctgcttgt cctggcctgg aacgacgttt cgcgctctgg
     7261 acaacctagc gacttgtttc accgaaggca cctcccagcg ctcagagcgt gagttgtgct
     7321 gcgatcggcg ctcctaccac gccgaaatcg acaggctcgc acagatccat catatcaggg
     7381 atggcgccgc ggacggatga aaggatgcgc gtgccaacat cccgcacctt cacaagcgaa
     7441 acagcagcgc caagatcagc ttggtttcgg cgtttgccag atcgggggtc cagccgctcg
     7501 gtgcacaggt cgatatggtg ccgtccggac tgtaaagacc accgcacttg tacccatctg
     7561 ggccggtgac tccgcctgga ccggcgtagt acggaaccga tgcttcatga tgcgagactt
     7621 caagtcgcac agtcaggttg tcgcttgggt aaacgcttag attcgatgaa acatcccagc
     7681 caaaaaattc cgtaccgggt cgggtgtcaa acgttgccgc agcgacgcca gtgggaacca
     7741 gtaccagata cctccccgga ttgcggataa agccgcctcc caacaggaaa ccaaagcggt
     7801 ttttccaaaa ccacaggttg tgatacgcca tcgcagacag aaagtcctgc tcgcacggtc
     7861 gctccttttg acagttgccg ggatcatgaa cgccgcggaa tgcagtgact ccgtctcctt
     7921 cctcgaatcc cagatcgaat gtgaatgaga cggcggcgcg actgattccc ttgctttcgc
     7981 tgcgattcag gtaacgcagc aggatgctgc tgtcggagtg aaaacgaact ctgcttgcgc
     8041 tcccttgggt atcggttcca acgtatccgt tagagaccat tttgatccac tcacgcggcg
     8101 cgtagtgaag ctggtatccg acgccaggaa gctgattgaa cttgccatag gtctgccagc
     8161 cgttgatcag ccacagctcg actttgagtc gctcggtcgg gaaaatctga agccgcgcgc
     8221 cattaaaaaa ccacggtgtg ttgtccgaag taaaggacgc ttggtagccc cagttttcaa
     8281 actgtgtata ggaaaacaga cccacatagg aaaagaacag tccgacgtcg acgttgatcc
     8341 catacagctt gtcaaagtga tacccggcat acgcttcgct taggtagcgg tagacactca
     8401 gcagatcgaa ctggccgcga ttgacggtca gatcgttgcg cggcaccgcg gaagatcggg
     8461 tgccaaactg ggtaaagatt ctggcgtgca cattcccgac atgcacatcg ccacctatgc
     8521 ccgcaaaagc gagcgacaac tcgttgtgtc ttgccgtcgc tgtcgatccg acaatcgtgt
     8581 tgtctatcgg ctggtttagg gaatacgtgt agtttgaatc aatgtcgatc tggggggtga
     8641 aatacttgct gtccaaaagg cgcgaggtct gtcggcttcc gccgtttatc caggtaaaat
     8701 cgccccacga aaatggttcc acagggcgat cgaaatcgct cggctggctt cccgaacctg
     8761 tttgcgttgc tgctttttct tcgcgcagtc ggcgcagctc gtcttcaagt ttgatcaggc
     8821 gttcttcgat agaaagtggc ggcttcagat cgactctggg aggatcggtt gtgtgtgtct
     8881 gtttgatgtc tgtactggga cggacttgtg cagacgcggg gctctctgtc tgcgcgtgcg
     8941 cgcgatcgca gaaaaggacg agcagagaca gcgcgcaaag cgtgatcttt cgatacatag
     9001 ggggcgctcc tgttttcagt gcacgaaaac gacgtgattt catcgcaagc gatgcgccac
     9061 ggtgatttcg ctgggatgac aggcgaattt gtcggaaaaa gcaagaaatg tgcagcggcg
     9121 gtctagcgat tcgggttgcg cgggatggga attcgcaaca gagcgctaac cgagcacggc
     9181 gcgaatcttt tccgcggcga tgcagctata ggccatgatg gtcaggctcg gatcgacgct
     9241 gggcgcggtg ggaaagacgc tgggatcggc aacatagagg ttgtctgttc catgcacccg
     9301 atgatccatt ccgaccaccg atgtcttggg gtcttttccc atccttgcgc ctcctgcggg
     9361 atgtggcgcg gcgaggacaa actttcctgg ggtgatgtca agcttcgcga ccgcgcgttc
     9421 cagctcgctc tctttggtga ttcgggtgtc gtgtacatcg ccaaagatca gctcctttgc
     9481 ccccaccgcg agcagcagct tggcctgttt ggcaaacgca tcgcgaatcc gcagaccatt
     9541 tccgccatgc aaaggaacgc gatatttggg ctgatccccg tcgagccgaa tgtttccttc
     9601 ttccacatca tcgatccagc agatggtgcc gccgagcttg cgcaggtttt tcatcaggtg
     9661 gcgatggctg tcaccaaaac caggaagggt ggccgccagc gtcgccggct gaagctggtt
     9721 tgccatcaac agataacccc cttcgatgta cctatcccct gcaaacgtcg ccaagcggaa
     9781 ctgatcgatc ccaaaggccg ctggaatgtt gcgaaactgg atgatctctt catcaaacag
     9841 cgcatgaacc atcggacagg ggttgcagta ggtgtgctct cccaccgcag ggagcttcgc
     9901 tttgagtccg tttcgcagca gaaataccgg tgttccataa ccacccgcag cgacgacaaa
     9961 tatcttggca tgtatgcgga ccatggcacc ggttgggcgt ccttcgccgc gatgcagaac
    10021 ccgcgccaaa agcgactgtg cgcggttccc attccaggac aactcgatcg cttcacagtc
    10081 ggcgtagatg cgcgcattgg cagcgagcgc tcgcggaatg tgggtgacaa gctggctctg
    10141 tttgaagtcg taggaacagc cctgcatgca gtagccgctc tttgcgcaag ggagtcgtgc
    10201 gatcggaacc ggggcccctg accagcccag cttgcgcgcg ccttcttcca agagctgatt
    10261 catccggttg cgaaactgcg gctcggcttc atgcacatgc agatcgcgct ccagtgtttc
    10321 aaagtgcggc gtcagatcgg cttccccgtg cccttcgatt ccgtactttt cagtccacag
    10381 cgcaatccga tcgcgcggcg ttcggtagct atccgcccag tagtgcacgc tggcaccgcc
    10441 gacgttgttg ccatacgtaa gctgcacgga catgtcttcg gatgtatcga gcccgcgacc
    10501 cccatcaatc ttggcgagca tgtcgtcttc ccgctgatcg aagtcttggc gggtatagga
    10561 accgccgcgc tcgatgaaga tgacttgctt gccggcttcg gccagacgga ctcccaatgt
    10621 cgcgccacca cagcccgttc cgatgatgca gacatcgcag gaatcgaccg cgcccgcatc
    10681 aagatcacga tacgttttga tgcttccgct catgacgccc tccgcttgcg caaaagatcc
    10741 tgatacagca catgggagtc tggatacacc ggctctttta caaacggacc gtcatagccg
    10801 atgtgacccc agacgctcgg atgcgcgtag taaaacagat gtaccacctg ttttatcgcc
    10861 agcacaactt ggcgcagcgc ttcctgcttt cccgtcgaaa gtgtctgcaa ataggtacgc
    10921 tgctggtccg gcgcgagcgc cgtaaaacga tgtgaataac catgtacgag cgtcgcatgt
    10981 tcaagaagtt gcaaacccgc tgccagagcc gatcgaatgg caggaggcgc gacataaaat
    11041 tcctcatcga tgcgctgtgg gattcccaat tcaacgccat cgggaaagcc atcccccgcg
    11101 ggggtcagcg tccgcacgat ggctttgacg atggcaaacg cctttgtgct aagcgcaagc
    11161 ggaatgtcgg aaggggagag ctgttccgca tagccatgtt ggaaatgacg cagcaggccg
    11221 aaagacaccg caagcagcgc cgcagagcca attccaatct gaaggaacag tcgacggttg
    11281 ggagggagcg acatcacgcc atgctacacc cagatggcgc tgcggtggcg ggatctggtg
    11341 gtgactgccc tgccacacca tcccacgcca tcataccgca cggatcgtcg tgcaggctgg
    11401 cacaaaatag tccgtgacag cgccctgggt tcaccgcacg atcaggatgg gaacgcccgc
    11461 ttccgtctgg cgttttttac cttcctagag ggctgacttg gggacgtcgg cacagataag
    11521 aaacactcgc aatgagtatt atttgcgggt tcggggtcag tttcctgatt ccatgccatc
    11581 tgtcgtttcc cctgtgtctg ccgcggaatc cgcgaccgag cctgccctag atcccgacgc
    11641 tgtgatcact gcgatcaggg cgatgcagga ttcgacatcg caagcgattc cggctgcttt
    11701 tccgccgcag tctgtgctgc tgcgatacgc gccaactgcg ctttcgcagg gctgctggtt
    11761 gtggcagagc gtgcaagtga aacagacgca cacggcgctg ggtggcgcgc ttgtggatgc
    11821 cttttttctg atgtgcgggg aaggggactt cgcacagcat cctgcgagcc aatatcgcgg
    11881 actcctttcg tctgtctcgg tctgcctgcc accgctttcc tcgcgcgcat ttgtggaaga
    11941 tccgcgcttg tgtgatgccg attttgcgct gccgctcttt ggactattcg tcggaaatca
    12001 gccagcttct gcgcttccgg atgtgctggg aattcatgcg gcgatgatgc tgtgggggcc
    12061 gccgcctgct gtctgtcaag cgatgaagaa ctgtgcttcg gcatcccagc tgcagagctg
    12121 ggaacagaac acagacagac aggagcgtgc gctggcgctg atcagaaaaa cgctgaccct
    12181 gtatgccgaa gcggggaatc ccaactggcc gcgcgtgctg gctgttgcgg caaagctgcg
    12241 tgacttgcgc agcgcatttg tggatgcact ggttccgagc gcgccgccga ctgcttggga
    12301 aaacatgctt gcgctggtac agcgaaagtt tcgccatggc tttggtttcc atcgccacgt
    12361 ccacctggca ggtcgatcac tggaccgctt ttttgatccc cagaagcctg atctgcaggg
    12421 attcctgatc gcgctcagcc gatcgcattt tgtgacccct ggacgacctg agcagagcct
    12481 gatcctgcgc cgaacgacgc agtttggtgg gacgatgttc ggcatctttg acgatcaaga
    12541 actggccgtc ctgtcgtcgt ggatcgctgc gcttcccgat tccgcggtgc cggaaatcgc
    12601 cacgccgcag gcgacgcaag atcacggtct agatgaacgt gacaatgaag gcacggcgac
    12661 gaaaacagag accgcatggg agactcttgg ggggtctccg tttggtggta ctgggcctct
    12721 gccatcgctt gatctgccgt cgctgtatca tcacttgctg gccgatgccg gtgatccagc
    12781 gtggaccgcg tgcgcaaaga cgtatcttgc cgagcgcatc accgaagtgg caaaagcggg
    12841 gtcggtggac catctgacca agcagaagct ctggccttat tctgcatcgc ggcttgccga
    12901 ttgggtggac gcgcagcttc gccagcaggt gcttgggaat ccagatgtcg gcggaaagac
    12961 agaaccccaa gaggacatgg gaatcgagcg gcacctgcac aagtccgaag tgctgtggtt
    13021 gctgacccag cttgcaccag ccgccctgat cgatggagcc tggctgcaag gaatcaccac
    13081 gccgcttttt tccgcgcaga tcgtcacgcc gcttttgttt cagatttatc gcgatgagct
    13141 gggggccggt gttccgcagc agcatcacgg caatctgatg cgaagcgtgc tggttgcgga
    13201 agggattgtg cttccggact gcgacagcct cgattttgtt cgatatccgg gcttccttcc
    13261 tgcgtcgttt tccaccccgg ttctgtggct tgcgatctcg cgctgtacgg attcgttttt
    13321 tccagagctg ctgggactga acctggcgat tgagctggca ggtgttggca agggctatga
    13381 tcgcgcgacg gctcttttgc ggaagcacgg aatcgatccg tatttctttg ttctacacaa
    13441 cacgatcgac aacggtgcat cgggtcatac cgcttggtcg gtgcgggcga ttgtcttgta
    13501 tttggaacag ctctgcgcga cggcggatca ggaagcggtg gcaacttgct ggcagcgcat
    13561 cttccgtggc tacgcgctgt acgcaaagtc cagtctgccg ctgctttccg cgatcggaat
    13621 gcggatggga ccgcgtctgg gaatgcgctg gttgtggcgt tttctgaccg gcaaaaccgc
    13681 ggcggagtcc agctagtcat gtgtggaatc gttggtgcgg tcgatctaca aggccggcga
    13741 aattttgatc cacagcgact tgccgacatg gccgcaagtg tccgtcatcg cggtccagat
    13801 gatgcgttcg cgtatcttgc gccgggaatc gccatggcga cgcagcgtct tgcgatccag
    13861 gatgtcgccg cgggtcgcca gccactgacg gatccgcggg gccagattgt ggtgtcccaa
    13921 aatggtgaat tgctcaatgc cgattcactg cgcagcgagc ttgcgcggca gggacattcc
    13981 ttccgttcgc actgtgatac cgaggtctgg cctgcgctgt atctgtctgc gaagaccgat
    14041 gcatttgtgc gcgcacaggg acagtttgca gtggcgctgt gggatcggcc agatcggacg
    14101 ctgtatctcg cccgcgatcg agtgggaatc tgtccgcttt actacaccgt ggcgcaggga
    14161 tatttgctgt gggcatcgga aatcaaggcg ctgctggcat cgggtcttgt gaatcctgaa
    14221 gtggatcgcg aaggaatcga tctggtcttc acactgtttg cagcagggac caaacggact
    14281 gcatttcgca acatatgttc cctgtggcct ggtcactatc tgcgagtacg ttcaggcgac
    14341 atcgcgacaa tccgttactg ggatctcgac ttcccaccac agggtgagga gcgcaaagat
    14401 cggacagaaa gactgaccga tgagctgacc cagatcgtcg attctgcggt gtcccgccgc
    14461 tttgcaagtg atggtccggt ggcgacgtat ttatctggcg ggctcgattc ttcgctggta
    14521 ctggcgacgg cgagtcggct tcgtccgctg cagccgctgc attcctttac ggtgggattt
    14581 gatggtgcag ggcccgatga gaggccgcag acgcagcaaa ccgcagcgct cttgtccgcc
    14641 aagctgcaca cgctggtccc gtccgcgcgc gacattctgg atgcacttcc ttcggttgtt
    14701 cttgccgcag agggtccgtt tatggacacc gccaacgctt gtcttttgct gctggccaag
    14761 caggtcgccg ccgcaggatt caaagttgtg ttgacaggcg agggcgcaga cgaagcgatg
    14821 ggaggatacg tctggcacaa gacggccaag ctgtttcgct ggctgtccaa actgcatcct
    14881 ggtctgccca gttggatccg gcgcagcgtc gcagaatatg ccatgcaggg aacaccccaa
    14941 gcgacttggg ggaatcggtt tgggaatccg cagccgagtc tcttcgatgt gtatgaaccg
    15001 ctgtcgcgcg cgcgctcgct tttgtattcc gatgcgatga ccgcggctgt atctgcgcac
    15061 gatccctttg cggatctgga catttcggtc gaccacatgc gggcgtggca tccgctcaac
    15121 cagtcgctgt atctggaata caagctgatg cttccggggc accttctgct gggcaaggga
    15181 gaccgcgtgg cgatgcaatc tggcgtagag gcgcgctatc catttttgga tgagtcgttt
    15241 gtcgcgttct gtagtcagct cgcaccggaa tacaagctct gtggttttac agaaaagtgg
    15301 ctgctgcgca gagtcgcaca gcgtgtcctg cccgcgaaca tgcgacagcc gcaaaagagc
    15361 atgttcaagg ccaactccct ttgccttcta aaaccgcagc cagcctggat tgatcagctt
    15421 ctgctgccgg aatcactgcg caagacgggc tacttttccg tcgaaaaagt cgcgcacgaa
    15481 agggcgctcc aagcccagct tcccgacttt catccgcggc gctatttggt cgatggcagc
    15541 ttcactgcgg tggtcatgac ccagctttgg catcacctgt ttctgggggg cgggctctgt
    15601 gagcttgctc cgtttcgggt ttcggaatcc tcaccacttc cgtttttttc tgacgcagga
    15661 aatgaacatg caaactgaac gcatcgggca tgaaggccgc cttcttgatc tggtcagtcg
    15721 tcgctgggac aagctagact ggcgcacgcc gattcctgtc gatcgacttg tcatcaccga
    15781 atcgcactac atggatgtgg agtcgcttgg ctttgatccg cggggccgca atgcgatcaa
    15841 tcggctgctc gcctgcttca cctgcgagct gttcattcac tttgaacgct atgtgatcga
    15901 gtttctggaa catcgcagac acgaaattcc gtttgttcct gcggctgcac tcgatcgctt
    15961 cgttgccgag gagaaagcgc actccgacgc attttatcgg ctgctggtca agctgcgtcc
    16021 ggatctgtat tcgacaaaag ccacaacgac cactggactg cgttttatgc actggacgct
    16081 gggcgatgat gcggcgatcg cgctggctcc ggtgggttcg ttttttttgc tcgcgtggct
    16141 ctttgaagaa atcacgatct acgttccggt
//
LOCUS       DAORGN010000112        16164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1480_length_16164_cov_6.228133, whole genome shotgun sequence.
ACCESSION   DAORGN010000112 DAORGN010000000
VERSION     DAORGN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16164
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_16164_cov_6.228133"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..97
                     /locus_tag="PKL17_12995"
     tRNA            2..97
                     /locus_tag="PKL17_12995"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Sec,seq:tca)
     gene            complement(377..571)
                     /locus_tag="PKL17_13000"
     CDS             complement(377..571)
                     /locus_tag="PKL17_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45699.1"
                     /translation="MLETVARALGSFTQEVVFVGGATVSLYLQNPASPEVRPTDDVDC
                     FIELSSYSSYAEVVERLAKL"
     gene            complement(589..1164)
                     /locus_tag="PKL17_13005"
     CDS             complement(589..1164)
                     /locus_tag="PKL17_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45700.1"
                     /translation="MRSQAGMKPQDVLVLLKLVVWKEKFVRLPSGWQGYRRRSGSWRQ
                     LDLASELGLSQAEVTHSLNRSRVARLIDADKREPMRLALCDFLVHGLQYVFPVEPGGI
                     VRGMPTAHSAPPLSTMIPEQEERYVWPDEQGEVRGQAITPLYPTVAEAARRDPKLYEL
                     LALLDALRVGRAREVRMARDELRKRIVESAQ"
     gene            1359..2339
                     /locus_tag="PKL17_13010"
     CDS             1359..2339
                     /locus_tag="PKL17_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45701.1"
                     /translation="MRQKERTLLLWLALIAMFAGAYSALSDKGHGPSLATFVSDAESA
                     QVSSVQLEPDTDSADRVVLRVLRKDGRRYETSPIALTAAIESVQSGGIPYSLKPAGRI
                     DTTFWLSLIPVLLIGFFVFYFVRSLKGLGSGKSPGVTDIVQARIEVQPPPSLAKPSFD
                     SEPAQRFLASVQDFKDNKDGPRKLLLCGPPGSGKTTLVLNAAFASQLSYIALPASDVA
                     NMFVGIAAARIRRAFELAHEKAPCLLLIEDMDGMAARRTIPGEGKAAEEGQLTEQVQG
                     MLELCAILDGVREFPSGVLFVATSNRPDRLDEALVRKGRIDLRIDLPAPP"
     gene            complement(2379..3068)
                     /locus_tag="PKL17_13015"
     CDS             complement(2379..3068)
                     /locus_tag="PKL17_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HNK45702.1"
                     /translation="MIWERVKPWGTRAAAAVLFRYVCFVAMIALSLWAESRPAPHLPD
                     LLIDRIPYVESVDRYNYWLLAAGYVPMAVWLLVRDPKRFCRYCVSAGLLSLLRGVCIV
                     VTGLGPVRGPDLHAGMFDRDPTLFARALLDMASPLGLLLRDAPHVYLTKDLFFSGHTA
                     ATCLLLLYVWPFVTLRRLMLATHIAVVASVFFAHLHYTIDVIGAYAMALAMFALREGW
                     PQGLRLHDGAT"
     gene            complement(3096..3914)
                     /locus_tag="PKL17_13020"
     CDS             complement(3096..3914)
                     /locus_tag="PKL17_13020"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNK45703.1"
                     /translation="MSNLVLRFLTAVVAVPLLILAIQWKEPYGVWIWVLIAQVTGLRE
                     WMNMTLPKDPALTRWLGVVAGVAFGNLLCWVPSAHLQQFGLFAVTGFGLLYFLFAHGN
                     IETVAARMSMWMLGVLYAGVLIVPLVMFKTAPKLGGGFIYVCLSSAWGSDTGAYFAGR
                     FLGPYIPAKLWPEVSPKKTWIGSVGGIFGSLLALTIAKLWYLPSLNWLDVFAIAIPAN
                     ILGQLGDLIESLIKRSVGVKDSGVLLPGHGGMLDRIDALLLVVPYVYVYAFLRF"
     gene            complement(3907..4647)
                     /gene="uppS"
                     /locus_tag="PKL17_13025"
     CDS             complement(3907..4647)
                     /gene="uppS"
                     /locus_tag="PKL17_13025"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNK45704.1"
                     /translation="MGTRPLLPPGLRHVAIIMDGNGRWAQQKGLPRLEGHRRGARSVR
                     EVVRACRELGLSALTLYAFSEQNWERPLEEVQGLMQLLHDYVIDERSEILENGIRLQA
                     IGNISRLPEYVRNPLSSLIEESAHNRKMVLSLALSYGGRESLADAMQKLAQDVAAGVL
                     DETALDEKALSARLKTAGLPKVDLMIRTSGEQRTSNFLLWESTGAQFYATSVLWPDFG
                     KAELLAALSVYRTHEGAEASAQLGSGDV"
     gene            4731..5939
                     /locus_tag="PKL17_13030"
     CDS             4731..5939
                     /locus_tag="PKL17_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNK45705.1"
                     /translation="MSVHFSSRLPWGRPPNRVSAKLQALKSQGVSLLDLTVSNPTRAL
                     PDLYPQTLLSSLSSPQALRYEPSAQGALTARESIARYYQDRGYTVDPQHIVLCASTSE
                     AYAWLFQLFGNPGDQVLFPEPSYPLLPLLGQLTSVETVPYPLRFDGRFHQSLPELGAL
                     LTPHSRAIVVVSPNNPTGNYLRQAEAAGLLGMAAARNIPLLIDEVFSDYAFSDPCPQE
                     GPRLLSLCQLPSPLADIPALRFVLSGFSKVLGLPQLKLGWIVVAGPEPLRSLAQDRLE
                     LIADTFLSVGTPIQLAAADLLAQRTTLTAGISARISASLQTLQTVFADTMVSVLPVEG
                     GWYAVLKVPTFVPEEELVLRLLDDDHLIVHPGYFFDFAAESYLVVSLLCPPADLADGA
                     ARLLHRLENV"
     gene            5936..7246
                     /locus_tag="PKL17_13035"
     CDS             5936..7246
                     /locus_tag="PKL17_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNK45706.1"
                     /translation="MKKSPLFTVLSTVFLFYTGAAWAEPPADPIGAPTEPVASNANPA
                     PATAPAPTVRPAAPALDATGPGPVIVESSLGKLTLGGYAEGFYQWNFNRPPSGITNYR
                     GFDNRHNSFTIANAAIDAQYEKSGVTARLALQIGHTPNTYYLAEPVSRADLSTSVVGS
                     SDANTWKYIQQALIGYKVPLGRGLLIQGGVFLSPIGPESLIVKDNWTYSRSNLFFNLP
                     FYHTGVRLTQQLSEAWSATIGVVNGWNSVVDNNSEKTLYFHLYFNRPDRLQFSVLYFT
                     GVERNPLDATRSWRHLFDAWSTLYFGNRFAFSLHMNVGFEPEQDNTASWIAGATYVRV
                     GIVRWLKLAARGDVFWERKPSSFASPNYPIFFAPSDLTWMASGTSTLEFGGVPGVLLR
                     LEYRHDHADGNIFFAAPTDGLFTSGRDQSKNSQDTITVGAVAWF"
     gene            7485..8849
                     /locus_tag="PKL17_13040"
     CDS             7485..8849
                     /locus_tag="PKL17_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNK45707.1"
                     /translation="MNAPIKYLLDETRLPKSWYNLAADLPTPLPPVLHPGTRQPIGPA
                     DLEPLFPMALIEQEVSTAREIEIPEPVQRVYRQWRPSPLFRARRLEQALDTPARIYYK
                     YEGVSPSGSHKPNTAVAQAFYNQQAGIKKLTTETGAGQWGSSLAFAGSLFGIEVQVFM
                     VRVSYDQKPYRRALMETYGARCIASPSSETQSGRAVLASNPYHPGSLGIAISEAVEIA
                     AQRADTKYALGSVLNHVLLHQTIIGQEAIAQLEMAGDYPDILVGCTGGGSNFAGIAFP
                     FLGVQLRGGKKVRIIAVEPAACPSLTRGKYAYDFGDTGKLTPLVKMHTLGSTFTPPGF
                     HAGGLRYHGMAPMVSHLAELGLIEARAYHQVSCFQAGVQFARAEGIVPAPEANHAVRG
                     AIDEALRCKEEGVAKTILLNLCGHGHFDMQAYMDFFAGKLKDQSYSEEELALALAGLP
                     SEQP"
     gene            complement(8846..10093)
                     /locus_tag="PKL17_13045"
     CDS             complement(8846..10093)
                     /locus_tag="PKL17_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45708.1"
                     /translation="MTVRAKRVLLVGILALLFGLGFVLRPDGPLGHKVWPAEAIEPHV
                     SASGNQVALAFMEVAGSGEVATAIAVRGSHDGGQTLGDVEQLIAPGHRLSADPTLAFA
                     PDGSLYVSWLSFRSHSSIESEPFDMAIVVARRRPGQAHFDPPMLVARDPQTTYDKPWS
                     SVDRDGIFHVVFRSAFANRAGIHEVALAADGRLSTRKLIDEPGFSGSLPTVCSDPVSS
                     QSYVAYLRPERGIELLSFDKNTVVPPQLVSNDGERIANAGPSCVATPSSVVVAYGLCD
                     SPWDSARSPQPKALVLVQYRGPGIKNVRKEIGQPGLIMMYPQLLPSLVGQDSQLQLAF
                     LAGHRDGDPDATLRLLSLPVDLSQDVPPRVDTLRRGLRMTASREDRRWPGDYLGIARS
                     DGHTVISYIDNSGRRHARLAIQP"
     gene            complement(10112..11689)
                     /locus_tag="PKL17_13050"
     CDS             complement(10112..11689)
                     /locus_tag="PKL17_13050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45709.1"
                     /translation="MGKEQEKICISCASSYPAAYSSCPRCQLPLVFPNIGKVWHVDKL
                     IGRGGMGAVFLAHHIDDPARLSAVKVLQLVVGEQRADRQEKVSRFQREAEALGRLKHP
                     AIVELYDFARERDGSLYMAMEYLQGVPLSRVLKEQGPLAPADAVSLILQVLAAVGAAH
                     RVGIIHRDLKPDNIVVLRDPELPDGSVRFDRVKVVDFGVARLKDDPATEAGQALGTPV
                     YMAPEQAQGGEIDERVDIYGAAAVLFELLAGRPPFLPPDGPNANLLLLAKIMTQDPAP
                     LRELRPEVSPALESVIERALCRDRTLRYPEVVDFSQALHAALGQPATRLWARPQVVAP
                     LSLPPLVAQAVSPSRPASLSRTLPPPVRSSASLPPVMRSGPTSLSRSSIGTAAISRSM
                     AGGAGLSQSTSALNSLSSLRGDGVPASSLSSAPSGPYLGGISGAHPLPSSSLSGMRSG
                     AVALPRSGVTPPPSDSSRSLSSGAGQQRSITYRILIALLYLAAVSILVFSLLFDRSSC
                     RGNRDRASSLPPAIGGP"
     gene            11775..12617
                     /gene="ttcA"
                     /locus_tag="PKL17_13055"
     CDS             11775..12617
                     /gene="ttcA"
                     /locus_tag="PKL17_13055"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNK45710.1"
                     /translation="MNPSTPGKMQLLRTMQRTMKSYALVSAGDRIMVAVSGGKDSYTM
                     LDLLCQAQKRAPFRFELCAVHLDQGQPGYDGKPLADWLESFGAPYEILREDTYSVVKQ
                     LVKEGDTYCAPCSRMRRGVLYSAAERLGCNKIALGHHREDTLQTLLLNLLYAGKLQAM
                     PAKYLTDDGRFEVIRPLIDCSEREIAAHAKAAGYPILPCNLCGSQDGLRRKQVESLLD
                     QLETTIPDVRQVMLHALKNVRPSHLLDQDVAAAWAERRPELRPDLRASVPLALRPGHL
                     PILD"
     gene            12884..13810
                     /locus_tag="PKL17_13060"
     CDS             12884..13810
                     /locus_tag="PKL17_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45711.1"
                     /translation="MQPADMSMGGCDPVSGMCDMAKPPGDMAMPPGDMAMPPGDMAMP
                     PGDMARPDGGGPVDMTVPPGDMARPDGGGGAGGIGAPCTADTDCKAGPTPSCWKSNVL
                     NNTMNPATPGGYCSSECTSDAACGAGARCVSLGMKSYCLASCNNATTCRHPGYACSYY
                     DTAGVCFPDTIFDCSPKTASCTEAGTGKMGGCIRQAYEDKGTCAASCTAGTGTCASVG
                     STKRQCVYLDTTKSPPNDAWKGLICLESPATAVTPGGACTYVNDCTDGYQCDGASSTC
                     RQICVKGGTPACTMGMCTDVFGTAATGPGICR"
     gene            13903..14481
                     /locus_tag="PKL17_13065"
     CDS             13903..14481
                     /locus_tag="PKL17_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45712.1"
                     /translation="MSSGTPHLRICPPPSSEPAVADHALPVDTRPLTIERLFVEHAGF
                     VATVVLRVLGRDHEVDDVVQEVFLDAMSGLRSIQNPAAVRGWLKTIAVRKASRHLRKR
                     RLRAFLGLERDCCYEHLADPSCGPEERALLSRVYRQLDTLPVGDRVAWTLRHVDGEPL
                     DSVAALCGCSLATVKRRISAAQAVLERRLRHG"
     gene            14474..15676
                     /locus_tag="PKL17_13070"
     CDS             14474..15676
                     /locus_tag="PKL17_13070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNK45713.1"
                     /translation="MADLFSRIHAAAGHISADWNPARASEVLGQMRKTHKRRQVRKVV
                     GGSLALLLICAGAWVVSRRQHLPTQIGPEPQIAQQSDGQVRLGDGSLARVLDEQSHLE
                     LITTGPDKVLIRLRGAASFQVAKNPQRLYRVETERVAVEVLGTRFIVKEQADHRVHVA
                     VSEGRVRVLWDKHQTELAAGTSGDFPRPLTAPTGLAQAEPAPIQADEPGIMPPQHRPL
                     TVLSPRTPRGTSQAKVAPPLPTPTVEPWQSLAEQGKFEQAYQQILSQSAMVDFLRRGS
                     IDAAELLLLADVARFSGHPSDAVPPLRQLLRDSPKDPRAPLASFTLGRILLDDLGQPR
                     EAAQSFRHVEDLEAEGPLTQDALAREVEAWSRAGEQGVAHSRAEEYVRRYPTGRRLRS
                     VRRYGDLD"
     gene            15697..>16164
                     /locus_tag="PKL17_13075"
     CDS             15697..>16164
                     /locus_tag="PKL17_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45714.1"
                     /translation="MVLLASLAAGAARADAGSNDVPQVALDIDPCVAVSQAEVQRRLA
                     LEIGERVISLGEASPTSSHAAATCDESGRITLRIDDPLTRKSIWRSIDLTSQEPSVRA
                     RLLAVAIAELLYASFAELLLEKRTEPDKPKPAQPPMPVAVVQTWSGRVEDKLKQ"
BASE COUNT         3241 a         5166 c         4801 g         2956 t
ORIGIN      
        1 cgggagcgtg tccgggactg gtgcccggcc cagtcttcaa aattggtggg accggtgtaa
       61 gaaccggttc ggcgggttcg attcccgtac actcccgttt tcgtcgctgc ggtggggatt
      121 agaaagtagg ctaaagcggg ggtcttctcg gtttgccagg cacgatgagt cgcatgacct
      181 cctcgcgcgc aagcgctgtt tcacaaaggg cgccgttcca catcatgtgg accgggcgaa
      241 tttctgaaac gacccctcct atcgaaaact cgataccaat atcgaaattt cgatattttt
      301 tcgccatcac cgagggtagt tgctgtcgag cctgtacgga tttttccgct tcatacggtt
      361 gttggccgga ttgccatcac agcttcgcaa ggcgttccac gacctctgcg taggacgaat
      421 agctggaaag ctcgatgaag cagtcgacgt cgtcggtcgg tcgaacttct ggggacgccg
      481 gattttgcag atagagcgac acggtggctc cgcccacaaa caccacttcc tgcgtgaagg
      541 agcccagcgc gcgggctacg gtttccagca tgttcaggtt cggtgaactc actgcgcaga
      601 ctccacaatg cgttttctca gctcatcgcg ggccattcgg acttcacgcg cccgtccgac
      661 acgcagtgca tcgagcagcg ccagcagttc atacagcttc gggtcacggc gtgcagcctc
      721 ggcgacggtc ggatacaacg gcgtgatggc ttggccgcga acctctccct gctcgtcggg
      781 ccaaacgtag cgctcttctt gttcaggaat catcgtcgag agcggcgggg ccgagtgcgc
      841 cgtcggcatg ccgcgcacaa tcccgcccgg ctcgaccgga aaaacgtact gaaggccgtg
      901 gaccaagaaa tcacacagcg ccagacgcat gggctcgcgc ttgtccgcgt cgataagccg
      961 cgccacccga ctgcgattga gcgagtgggt gacttcggcc tggctgagtc ccagctcgct
     1021 ggcaaggtcg agctgacgcc aagaaccgga ccgtctgcgg tagccttgcc atcccgacgg
     1081 caacctgacg aacttctctt tccagacgac gagcttgagc aagacgagaa catcctgcgg
     1141 cttcatcccg gcttggcttc gcatggagag aatctagcgc tcagtgcata gatttacaat
     1201 ttgtaaatta cgaatcgtaa atcatcaacc tactcatctt cgcagcgacg ggtggagcgt
     1261 cgctcgcctt gcccccccgc cgccgctccc cgcgctgacg cgagtacact cccgttttcg
     1321 tcgctggggt ggggggattg cgtggtaggc atacggtgat gagacagaaa gaacggacgc
     1381 tgctgctctg gttggctttg attgcgatgt ttgcgggggc ctattccgcg ctttccgaca
     1441 aaggacatgg accgagcctg gcgacgtttg tctcggatgc agaaagcgcg caggtcagca
     1501 gcgtgcagct tgagcccgac acggacagcg ccgatcgagt cgtgctacgg gtgctgcgca
     1561 aagatggccg ccgctacgag accagcccta tcgcgctcac tgcggcgatt gagtcggtcc
     1621 aatcgggcgg aattccctat tcgctcaagc ctgctggacg gatcgacacg acgttttggc
     1681 tgagcctgat tccggtgctg ctgattggtt tttttgtctt ctattttgtc cgcagcctca
     1741 agggactggg cagtggcaag tcgccgggcg tgaccgatat cgtccaagcg cggatcgaag
     1801 tgcagccgcc tcccagcttg gcaaaaccgt cgtttgattc ggaaccagcg cagcgctttt
     1861 tggcgagcgt gcaggacttc aaagacaaca aagacggtcc gcgcaagcta ttgctctgtg
     1921 gtccgccggg gagtggcaaa acgacgctgg ttttgaacgc ggcgtttgca tcgcagcttt
     1981 cctatatcgc gctgccggca agtgacgtag ccaacatgtt cgtggggatt gctgcggcgc
     2041 gtatccgccg cgctttcgag ctggcacatg aaaaggcgcc gtgtcttttg ctcatcgaag
     2101 acatggatgg gatggcggcg cggcgaacga ttccggggga aggcaaagcc gcggaagaag
     2161 gacagctcac cgaacaggtg cagggaatgc tggagctgtg cgcgatcctc gacggggtgc
     2221 gtgagtttcc gtccggcgtg ctgttcgtcg cgacatcgaa ccggcccgat cgactcgatg
     2281 aagcgctggt ccgcaaagga cgcatcgatc tgcgcattga tctgccggcg ccgccgtagg
     2341 aatccgaaca tcccgcggct tttggcgcag cggctgcgtt acgttgcgcc gtcgtgcagc
     2401 cgaagtccct gtggccagcc ttcacgcagc gcaaacatcg caagcgccat cgcataggct
     2461 ccgatgacat caatggtgta gtgcaggtgc gcgaaaaaca cgctggcgac gacggcgatg
     2521 tgggtcgcaa gcatcagccg gcgcagcgtc acaaacggcc agacatacaa aagcaaaaga
     2581 caggttgccg cggtgtgtcc ggaaaaaaac aggtctttgg tcaaatagac atgcggtgca
     2641 tcgcgcagca gcagtccgag cgggcttgcc atgtccaaaa gcgcacgcgc aaacagagtc
     2701 ggatcacgat caaacatccc cgcatgcaga tccggaccgc gcaccggccc aagcccggtc
     2761 acgacgatgc agacgccgcg cagcaaagac agaagaccgg cgcttacgca gtaacgacaa
     2821 aaccgcttgg gatcgcgcac aagcagccac accgccatcg gcacataccc cgcagcgagc
     2881 agccagtagt tgtagcgatc gaccgactcg acatagggaa tcctgtcgat gagcagatcg
     2941 ggcaagtgcg gcgccggacg gctttccgcc cacagcgaca gcgcaatcat cgccacaaag
     3001 cagacatagc gaaacagcac cgccgccgcc gcgcgtgtcc cccacggctt gacccgttcc
     3061 caaatcatga gctgaaaccg cgaccgcgtg cgcggctaaa agcgcaggaa agcgtagacg
     3121 tagacgtagg gcacaaccaa aagcagcgcg tcgatgcggt cgagcattcc gccgtgaccg
     3181 ggcagaagca cgcccgagtc tttgacgccg accgagcgct tgatgagcga ctcgatcaaa
     3241 tcgccgagct gcccgaggat gttggccgga atcgcaatcg cgaagacgtc aagccagttt
     3301 agcgacggca gataccacag cttggcaatc gtcagcgcga gcagactgcc aaaaatgcca
     3361 ccgacagatc cgatccaggt ttttttcggg ctcacctccg gccacagctt ggccggaatg
     3421 tacggaccca aaaaccgtcc ggcaaaatac gcgccggtat cagaacccca cgcgctcgac
     3481 agacagacat agataaaacc accgccgagc ttgggcgctg tcttaaacat cacaagcggc
     3541 acaatcagca cgcctgcata cagcactccg agcatccaca tcgacatccg ggccgcgacg
     3601 gtttcgatgt ttccgtgcgc gaacaaaaag tacaaaagcc caaaccccgt caccgcaaac
     3661 agaccaaact gctgcaggtg cgcggaagga acccagcaca gcaagttgcc aaacgcgaca
     3721 ccggccacca ctccgagcca gcgcgtcagc gccggatctt tgggcagcgt catgttcatc
     3781 cactcgcgca ggccggtcac ttgggcaatc agcacccaaa tccacacgcc atacggctct
     3841 ttccactgaa tcgccaggat cagcagcggc accgcgacca ctgcggtcaa aaaacgcagg
     3901 accaagttag acatcaccac tcccaagctg agccgacgct tcggctcctt cgtgcgtgcg
     3961 gtagacagac agcgctgcga gcagctccgc tttgccaaag tcaggccaca gcacggacgt
     4021 agcgtaaaac tgcgcgcccg ttgattccca cagcaaaaag ttgctcgtgc gctgctcgcc
     4081 ggacgttcgg atcatcaagt cgactttcgg cagccccgcg gtcttcagcc gggccgaaag
     4141 cgccttttca tctaacgcgg tttcgtcgag cacgcccgcc gcgacatctt gcgccagctt
     4201 ctgcatcgcg tccgccagcg attcccgtcc gccatacgag agcgccagcg acaacaccat
     4261 cttgcgattg tgcgccgatt cctcgatcag gctcgacagc ggattgcgca catattcggg
     4321 cagtcgactg atgttgccaa tcgcctgcaa gcggatgccg ttttcgagga tttcgctgcg
     4381 ttcgtcgatc acgtagtcgt ggagaagctg catcagcccc tgcacttctt cgagaggccg
     4441 ttcccagttc tgttccgaaa acgcatacag cgtcagcgcc gacagaccca gctctcggca
     4501 ggcccgcacg acttcgcgca ccgaacgcgc gcctcgccgg tgtccttcga gccgcggcag
     4561 ccccttttgc tgcgcccagc gcccgttgcc atccatgatg attgccacat gccgaagtcc
     4621 cggcggaagc agtggcctag tacccataag gcattggcgt atcacgaagc gtactccccg
     4681 gccaagaaaa agcccgctgc gcggtgtcgg cgtcgtgata atacccgcct gtgtcagtcc
     4741 acttttcgtc gcgcctgccg tggggaaggc cgcccaatcg cgtcagcgcg aaactgcaag
     4801 cgctcaaaag ccaaggcgtg tcgctgctcg atttgaccgt gtcgaatccg acgcgcgcac
     4861 tgcccgatct gtatccgcag acactgctgt cgtcgctttc atcgccacag gcgctgcggt
     4921 acgaaccgtc ggcccagggc gccctcaccg cgcgtgagtc gattgcccgc tactaccaag
     4981 accgcggcta caccgtcgat ccccagcaca tcgtgctgtg cgcgagcacg agcgaagcct
     5041 atgcctggct gtttcagctc tttggcaacc ccggcgacca ggtgctgttt cccgaaccaa
     5101 gctatccgct cttgccgctg cttggtcagc tcaccagcgt cgaaaccgtg ccctatccac
     5161 tgcgcttcga tggccgcttt caccaaagcc tgcccgagct tggcgcgctg ttgacgccgc
     5221 acagtcgggc cattgttgta gtaagtccaa acaacccgac gggcaactac cttcggcaag
     5281 cggaagccgc cggtctgctt ggcatggccg cagcgcgaaa tatcccgctg cttatcgacg
     5341 aggtcttttc cgattacgct ttttctgatc cctgtccgca ggaaggcccg cggctgctgt
     5401 ctctgtgcca actgccgtcg ccgcttgccg acattccggc gctgcgcttt gtgctatcgg
     5461 gtttttccaa agtgctcggt ctgccgcagc tcaagctggg ctggattgtc gtcgctggac
     5521 ccgaaccgct acgcagcttg gcgcaagatc ggttggaact tatcgccgat acgtttttgt
     5581 ccgtcggaac gccgatccag cttgccgcag cggatctgct tgcgcagcgc acgacgctga
     5641 cggcgggaat ctcggcgcga atttccgcca gcttacagac gctgcaaacg gtctttgccg
     5701 atacgatggt gtcggtcctt cccgtcgaag gtggctggta cgcggtcctg aaagtgccca
     5761 cttttgtccc cgaagaggag ctggttttgc gtcttctcga cgatgatcat ctgatcgtgc
     5821 accccggcta cttcttcgat ttcgccgccg agtcgtatct ggtcgtgagc ctgctgtgtc
     5881 cgcccgccga cctggcggat ggcgcggctc ggttgcttca ccgcttggag aacgtatgaa
     5941 aaaatcaccg cttttcaccg tgctatcgac ggtgtttctg ttttacaccg gcgctgcgtg
     6001 ggccgaaccc cctgccgatc caatcggcgc gccgactgaa cccgtcgcaa gcaacgccaa
     6061 tccggctccc gcaacagcgc ccgcaccgac cgtgcgcccc gcagcacccg cgctggatgc
     6121 cactgggccc ggtccggtca tcgtcgaaag cagtctcggc aaactgacgc tgggcggcta
     6181 cgccgaaggc ttttaccagt ggaatttcaa tcgcccaccg agcggcatca ccaactaccg
     6241 cggcttcgac aaccgccaca acagcttcac catcgccaac gccgcgatcg atgcccaata
     6301 cgaaaagtcc ggtgtcaccg cacgtttggc gctgcaaata ggacacactc caaatacgta
     6361 ttacctggcg gaaccggttt cgcgcgcgga tttgtcgacg agcgtggtcg gctcaagcga
     6421 cgcaaacacc tggaaataca tccagcaagc gctgattggc tacaaagtcc cgctcggtcg
     6481 cggcctgctg attcagggcg gcgtgttcct gtccccgatt ggccccgagt cgctcatcgt
     6541 caaagacaac tggacctatt cgcgttccaa cctgtttttc aacctgcctt tttatcacac
     6601 gggcgtccgt ctgacccagc agctcagcga agcgtggagc gcgaccatcg gtgtcgtcaa
     6661 cggctggaac tctgtcgtcg acaacaacag tgaaaagacg ctgtattttc acctgtattt
     6721 taaccgtccc gatcggctcc agttttcggt gctgtacttt accggcgtcg aaagaaaccc
     6781 gctcgatgcg acgcgctcgt ggcgacacct gttcgatgcg tggagcacgc tttattttgg
     6841 caaccgcttc gcgttcagcc tgcacatgaa cgtcggcttc gagcccgaac aagacaacac
     6901 cgcgtcgtgg attgccggcg ccacctacgt gcgggtcgga attgtgcgct ggctgaagct
     6961 ggccgctcgc ggcgacgtgt tttgggaacg caagccgtcg tcgtttgcat cgccaaacta
     7021 tccgatcttt tttgctccgt cggaccttac ttggatggcg tcgggaacat cgacgctgga
     7081 atttggtggg gttccgggtg tgctgctgcg gctcgaatac cgtcacgatc acgccgatgg
     7141 aaacatcttt tttgccgctc cgaccgacgg acttttcact tcgggtcgcg accagagcaa
     7201 aaacagccaa gacaccatca ccgtcggcgc agtcgcttgg ttttaatcag cgacggaaat
     7261 ctgttttagt ccaacagtag aaaccagccg ccgtggcgca cccaaaacaa tcgacgcagt
     7321 cgtagtcgta taggtcataa gcgagccctc cccaattttt ggtgcccacg agcgcgtcgc
     7381 cttcctgccg acctgccgat gcagagaaaa ctagaccgcc gacggaataa cggcctagcg
     7441 tggtcttgca gtcgatcgaa aacagccagc acaggagatt tgcgatgaat gctccgatca
     7501 agtacttgct cgacgagacg cgccttccca agtcttggta taatctcgcc gccgatctgc
     7561 caacgccgct gccgcccgtg ctgcatccgg gcaccaggca gccgatcggg ccggcggacc
     7621 tggaaccgct gtttccgatg gcgctcatcg agcaggaagt ctcgacggcg cgcgaaatcg
     7681 aaattcccga accggtgcag cgcgtctatc ggcagtggcg tccgtcgccg ctctttcggg
     7741 cgcgaaggct ggaacaggcg ctcgatacgc cggcgcggat ttactacaaa tatgaaggcg
     7801 tctcaccgtc gggaagccat aagcccaata ccgcggtggc gcaggcgttt tataaccagc
     7861 aagcggggat caaaaagctg accaccgaaa ccggcgccgg tcagtgggga tcgtctttgg
     7921 cattcgccgg ttcgctgttt ggcatcgagg tgcaggtctt catggtccgc gtctcgtatg
     7981 atcaaaagcc gtatcggcgg gcgctgatgg aaacctacgg tgcgcgctgc attgcttcgc
     8041 cgtcttcgga aacccagtcg ggacgggccg tgctggcgag caatccctat caccccggca
     8101 gcctcgggat tgccatctcg gaagcggtcg aaattgctgc gcagcgggcc gataccaaat
     8161 acgcgctcgg gtcggtgcta aatcacgttc ttttgcacca gaccatcatc ggacaagaag
     8221 cgatcgcgca gcttgaaatg gcgggcgatt atcccgacat tctcgtcggc tgtaccggcg
     8281 gcggatcgaa ctttgcgggg atcgcgtttc cgtttttggg cgtgcagctc cgcggcggca
     8341 aaaaagtgcg catcatcgcg gtcgagcccg cagcctgccc gtcgctgacc cgtggaaaat
     8401 atgcctatga ctttggtgat acggggaagc ttacgccgct ggtcaaaatg cacacgctgg
     8461 gatcgacgtt cacgccgcca ggtttccacg ccggtggtct gcgttatcac ggcatggcac
     8521 cgatggtgag ccacctggcc gagctgggtc ttatcgaagc ccgcgcctat caccaagtgt
     8581 cgtgtttcca agccggcgtg cagtttgccc gcgccgaagg aattgtgccg gcgcccgaag
     8641 ccaatcatgc ggtgcgcgga gccatcgacg aagcgctgcg ctgcaaagaa gaaggggtgg
     8701 cgaagacgat attgctcaac ctctgcggcc acgggcactt cgacatgcag gcgtacatgg
     8761 actttttcgc cggcaagctc aaagaccagt cgtatagcga agaggagctg gcgctggcgc
     8821 tcgccggtct gccttcggaa cagccttaag gctggatcgc cagacgggcg tgccgtcgac
     8881 cgctgttgtc gatgtagctt atgacggtgt gtccgtcgct gcgcgcaatt cccaaataat
     8941 cacctggcca gcgtcggtct tcccgactcg ctgtcatccg cagaccgcga cgcagtgtat
     9001 cgacccgcgg cggcacatct tgcgacaaat cgaccggcaa agacaaaagc cgcagcgtcg
     9061 catcgggatc gccatcacga tgtccggcca aaaacgcgag ctgaagctgc gagtcttgtc
     9121 caaccagcga cggcaaaagc tgcggataca tcatgatcag accgggctgg ccgatttctt
     9181 tgcgcacgtt ttttatgccg ggcccacggt actggacaag caccagcgct ttgggctgcg
     9241 gcgatcgcgc actgtcccac ggcgagtcgc aaagaccgta ggccaccacc accgagctgg
     9301 gcgtcgcgac acacgacgga ccggcattgg caatccgctc gccgtcgttc gagacaagct
     9361 gcggcggaac gactgtgttt ttgtcaaacg acagaagctc aatgccacgc tcgggccgca
     9421 ggtacgcgac gtaactttgc gacgatacag gatcgctaca caccgtcggc agcgatcccg
     9481 aaaaacccgg ttcatcgatc agcttgcgcg tcgacagccg tccgtccgcc gccagcgcca
     9541 cttcatgaat cccggcccga ttggcaaacg cgctgcgaaa cacaacatgg aaaatgccgt
     9601 ctctgtcgac gctgctccac ggcttgtcgt aggtcgtttg cggatccctt gcgacaagca
     9661 ttggcggatc gaagtgagcc tgccccggac gccgccgcgc gacgacgatc gccatgtcaa
     9721 acggctcgga ttcaatcgag ctgtgggaac gaaacgacag ccacgacaca tacagcgaac
     9781 catccggcgc aaacgccagc gtcggatctg ccgacaagcg atggcccggc gcgatcagct
     9841 gttcaacatc gccaagcgtc tgtccaccgt cgtgtgaacc cctgactgca atcgccgtcg
     9901 caacttcgcc gcttccagcg acttccataa aggccagcgc cacctggttt cccgacgcag
     9961 agacgtgcgg ttcaatcgct tcggcaggcc agaccttgtg cccgagtggt ccgtcgggac
    10021 gcagcacaaa accaagcccg aacagcaacg ccaaaatccc caccagcagg actcgttttg
    10081 cccgcaccgt catcgccgcg ccaagctgaa atcagggacc gccgatggct ggcggcaggc
    10141 tcgacgcccg atcccggttg ccccggcagc ttgagcgatc aaaaagaagg ctgaagacga
    10201 gaatcgacac cgcggcaaga tacaaaagcg cgatgaggat tcgataggta atcgaccgct
    10261 gctgaccagc tcccgacgac aaagaacgcg acgagtcgct cggcggcggc gtcacacccg
    10321 atcgcggcag cgccaccgct cccgatcgca tgcctgacaa agacgagctg ggcagaggat
    10381 gcgcgccgga aattcccccg aggtacggac cacttggcgc cgacgacagg gacgacgccg
    10441 gcacaccatc gccacgcagc gatgacaggg agttgagcgc cgaggtcgac tgcgacagcc
    10501 ctgccccacc cgccatcgaa cggctgatcg cggctgtgcc gatcgatgaa cgcgaaaggc
    10561 tcgtcggacc gctgcgcatc actggcggca gcgacgcact ggatcgaacc ggcggcggca
    10621 gggtgcgcga aagcgacgcg ggccgcgacg gtgacaccgc ctgcgcaacc agcggaggca
    10681 gactgagcgg cgccaccact tggggacgcg cccacagccg cgtggctggc tgaccgagcg
    10741 cagcatgcag ggcctgcgaa aaatccacca cctctggata gcgcagcgtg cggtcgcgac
    10801 acagggcccg ctcgatcact gattccaacg ccggcgacac ctcgggccga agctcgcgca
    10861 gcggcgccgg atcttgggtc atgatcttcg caagcagcag caggttggcg ttcggtccgt
    10921 cgggcggcag aaacggcgga cgcccggcga gcagctcgaa caagaccgcc gcagcaccgt
    10981 aaatgtcgac tcgttcgtcg atttcaccac cttgcgcttg ctcaggagcc atgtagaccg
    11041 gcgtgccgag cgcctgcccc gcttccgtcg ctggatcgtc ttttagccgc gccacgccaa
    11101 aatcgaccac tttgacccga tcaaaacgaa cactgccgtc gggaagttcg ggatcacgaa
    11161 gcaccacgat gttgtctggt ttcaaatcgc ggtggatgat cccgacgcgg tgcgccgccc
    11221 ccaccgccgc cagaacttgc aaaatcagtg ataccgcatc tgccggcgca agcggtcctt
    11281 gttctttgag cacccgcgac agcggcacgc cttgcaggta ctccatcgcc atgtaaagcg
    11341 acccgtcgcg ctcgcgggca aaatcgtaca gttcgacgat ggccgggtgt ttcagacggc
    11401 ccagcgcttc ggcttcgcgc tgaaaacgcg aaactttttc ttgccgatcg gcgcgctgct
    11461 ctccgacgac cagttgcagc actttgaccg ctgacagccg cgccggatcg tcgatgtggt
    11521 gggccaaaaa gaccgcgccc atgccaccgc gaccgatcag cttgtcgacg tgccagacct
    11581 tcccaatgtt gggaaacacc agcggcaact gacagcgtgg gcacgacgag taagccgccg
    11641 gatagctgct cgcacaggag atacagattt tttcctgttc tttgcccacg ttgattgtct
    11701 aagcgctaag cgcaaaaaag cggccctgtt ttgggcagct tgtctcatca atgtatcatg
    11761 cgaccccttt ggcaatgaac ccttcgaccc ccggcaaaat gcagctcttg cgcaccatgc
    11821 agcgcacaat gaaaagctac gcgcttgtct ccgcgggcga ccgcatcatg gtcgcggtgt
    11881 ccggcggcaa agacagctac acgatgctcg atcttttgtg ccaagcgcag aagcgagcgc
    11941 cgtttcgttt cgagctctgc gccgtacatc tcgatcaagg acagcctggc tacgacggca
    12001 agccgctcgc ggactggctg gaatcgtttg gcgcaccgta tgaaatcctg cgcgaagaca
    12061 cctattcggt cgtaaagcag ctcgtcaaag aaggcgacac ctactgcgca ccgtgctcgc
    12121 gtatgcgacg gggtgtgctc tacagcgcgg cggagcggct cggttgtaac aaaattgcgc
    12181 tcgggcatca ccgcgaggac accttgcaga cgctgctgct gaacttgctt tatgccggca
    12241 agctgcaagc gatgcccgcc aagtatctga ccgacgacgg acgcttcgag gtcatccgac
    12301 cgctcatcga ctgtagcgaa cgcgagattg cggcgcatgc aaaggcggct ggctatccga
    12361 tcctgccgtg caatctctgt gggtcgcaag atgggctgcg gcgcaaacaa gtcgaatcgc
    12421 tgctcgatca gctggaaacg acgattcccg atgtccggca agtgatgctg cacgcgctaa
    12481 aaaatgtgcg cccgagccac ctgctcgatc aggatgttgc cgctgcgtgg gccgaacgcc
    12541 gacccgagct acgacccgat ctgcgcgcgt cggttcccct ggccttgcgc cccggccacc
    12601 tgccgattct cgactagctt ttgtgcaaaa gtcgcgggtt tggttggctg acccacagct
    12661 cttctgtgac gatgaaagtt ttgctcgcac aggaaaggcg ctgtgggtta gggtgcgcgc
    12721 ccaggaggtt cctagaaaca tgatgagcct acgcggagcc aaggttttgg cagcggctgc
    12781 gtcgctcagc ggcgtatttt ttctttcagg ctgtccaaac cccccgcccc cggcggatat
    12841 ggctggcacg cctgacatga cgatgtccac cgaggacatg tccatgcaac ccgctgacat
    12901 gtcgatgggc ggctgtgatc cggtttccgg aatgtgcgac atggcgaaac cacccggtga
    12961 catggcgatg ccgcccggtg acatggcgat gccgcctggt gacatggcga tgccgcctgg
    13021 tgacatggct cgccccgatg gcggtggccc ggtcgacatg acggtgccgc ctggtgacat
    13081 ggctcgtccg gacggcggtg gtggcgctgg tggcattggc gcgccgtgca ctgccgacac
    13141 cgactgcaag gcaggtccca cgccgtcttg ctggaagagc aacgtcctga acaacacaat
    13201 gaacccggcc actccgggtg gttactgctc atcggaatgc acgagcgatg cggcctgtgg
    13261 cgcgggcgcc cgctgcgtaa gcctcggcat gaagagctac tgcctcgcga gctgcaacaa
    13321 cgcgacgacg tgccgtcacc ccggctacgc ttgctcgtac tatgacaccg cgggcgtctg
    13381 cttccctgac acgatcttcg actgcagccc gaaaacggcg agctgcaccg aagccggcac
    13441 cggcaaaatg ggtggctgca tccgtcaggc ctacgaagac aagggcacct gcgccgcgag
    13501 ctgcaccgcc ggcaccggca cctgcgcttc cgtcggcagc accaagcgcc agtgtgtgta
    13561 cctcgacacc accaagtcgc cgccaaacga tgcatggaag ggccttatct gcctggaaag
    13621 cccggcgacc gccgtgacac cgggtggcgc ttgcacgtat gtcaatgact gcaccgacgg
    13681 ctaccagtgc gatggcgcga gcagcacctg ccgtcagatt tgcgtcaagg gcggcacccc
    13741 ggcctgcacc atgggcatgt gcaccgacgt gttcggcacc gcggccaccg gccctggcat
    13801 ctgccggtag cgtcgcgcag cgcagcggtc cttgccgatc tctgcgctgc agcccggctt
    13861 gctgagccga ttcccgcctc ccaaaccact atgtcggtag cgatgtcttc cggcactccc
    13921 caccttcgga tctgtccgcc accatccagt gaacccgcgg ttgcggatca cgcgctgcct
    13981 gtcgacacaa gaccgctcac catcgagcgc ttgttcgtcg agcatgccgg ctttgtggcg
    14041 actgtcgtct tgcgtgtgct cgggcgagat cacgaggttg atgacgtggt tcaagaagta
    14101 ttcctcgacg cgatgagcgg tctgcgctcg attcaaaatc ccgcggcggt gcgaggctgg
    14161 ctcaagacca tcgcggtccg caaagccagc cggcatctgc gcaagcgacg gctgcgtgca
    14221 ttcctggggt tggaacgcga ctgctgttac gaacatctgg ctgacccaag ctgcggtccc
    14281 gaagaacgcg cgctgctcag ccgtgtctat cgccagcttg acaccctgcc ggtcggcgat
    14341 cgggtggcgt ggacgctgcg tcacgtcgat ggggaaccgc tcgattcagt cgctgcgctg
    14401 tgtggctgtt cgctggccac cgtaaaaagg cggatttcag cggcacaagc ggtgctcgaa
    14461 aggaggctcc gtcatggctg atctgttctc ccgcatccac gccgccgcgg gccacatcag
    14521 cgcggattgg aacccggcac gggccagcga agtgctcggc cagatgcgaa agacccacaa
    14581 gcgccgtcag gttcgcaaag tggtcggcgg atcgctggca ctgctgctca tctgcgccgg
    14641 tgcgtgggtg gtgtcacgcc ggcagcacct gccaacccaa atcggcccag agccgcagat
    14701 tgcgcagcag tcggacgggc aggttcgact gggcgatggc tcactggcgc gggttctcga
    14761 tgagcagagc catctggagc tcatcaccac cgggccggac aaagtgctga ttcgactgcg
    14821 cggcgcggca tcgttccaag tcgccaaaaa tccgcagcga ctttaccgtg tcgaaaccga
    14881 gcgcgttgcc gtcgaagtgc tcggcacccg cttcatcgtc aaagaacagg ccgatcaccg
    14941 cgtccacgtt gccgtcagcg aaggccgcgt ccgcgtgctg tgggacaaac atcaaaccga
    15001 gcttgcggcg ggaaccagcg gcgattttcc gcgtccgctg accgcgccga ccgggcttgc
    15061 acaggccgaa ccggcgccga tccaagccga cgaaccgggg atcatgccgc cgcagcatcg
    15121 tccgctcaca gtcctttcgc cgcgcacgcc gcgtgggaca agccaagcca aagtcgcgcc
    15181 gccacttccg acccccaccg tcgagccatg gcagtcgctg gcggaacagg gcaagttcga
    15241 gcaggcgtat cagcaaatcc tgtcgcaaag cgcgatggtc gatttcctgc gcagaggttc
    15301 aatcgatgca gcagagctgc ttcttttggc cgatgtggcg cgcttttcgg gacatccgag
    15361 cgatgcggtg ccgcccttgc gccaactttt gcgcgattca cccaaagatc cgcgggcgcc
    15421 gctggcaagc tttacgctcg gacgcatcct gctcgatgac ctgggccagc ctcgcgaggc
    15481 agcgcagagc ttccggcatg tcgaagacct cgaagcggaa ggtccgctca cccaagatgc
    15541 gctggcgcgt gaagtcgaag cgtggtcgcg cgctggggaa caaggggtcg cgcacagccg
    15601 ggccgaagaa tacgtgcggc gctatccgac gggacgacgg ctgcgatcgg tccgccgcta
    15661 tggggatctc gattgagagc gggcccacaa gcggccttgg tactgcttgc ttcacttgcc
    15721 gccggtgctg cgcgcgcgga cgcaggatca aacgatgtgc cgcaggtcgc gctcgacatc
    15781 gatccgtgcg tggcggtgtc gcaagcagaa gtccagcgcc gtctcgcgct cgaaatcggc
    15841 gaacgggtga taagccttgg cgaagcgtcc ccgacgagca gccacgccgc cgcgacctgc
    15901 gacgaaagcg gtcgcatcac cctgcgcatt gatgatccgc tgacgcgaaa atcgatctgg
    15961 cgcagcattg acctaaccag ccaagagccg tcggtgcgcg cgcgtctact cgcggtggcg
    16021 atcgccgaac ttctgtacgc cagctttgcc gagctgctgc tggaaaaacg caccgagcca
    16081 gacaagccca agccagcgca gccgccgatg cccgtcgcgg tggtgcagac ctggagcggg
    16141 cgcgtcgaag acaagctcaa acaa
//
LOCUS       DAORGN010000113        16128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1483_length_16128_cov_6.211099, whole genome shotgun sequence.
ACCESSION   DAORGN010000113 DAORGN010000000
VERSION     DAORGN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16128
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1483_length_16128_cov_6.211099"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1435)
                     /locus_tag="PKL17_13080"
     CDS             complement(170..1435)
                     /locus_tag="PKL17_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45715.1"
                     /translation="MDSAADKLDPGSPDNPQPFGKYLLLGLIARGGMAEVYRARSRQS
                     GGRLYAIKCMRSQLAKEARFVEMFVREGKLAVLLSHDNIVRTFEIGRTEGRYFIAMEY
                     IGGKDLTQILRRCQETGSRIPLPAACYVAAKMSEGLHHAHTLLDADGKPLAIVNRDVS
                     PSNVRLGWDGSVKLLDFGIAQAMVKFTSEIGVLKGKFSYMSPEQIRGMPVDARTDVFS
                     TGIILHEMLTTEKLFRGDTEFQLMERVRNAEVLPPSKFNRRVPEALDKIVMKALARDV
                     ADRYASALELAQDLQAFLAPYRFSDKEIVEQLKSLFRADYDQEMVEIAACQRAELPSD
                     GDRDASLVMTAAAIDGVAVGPGGVKLTANRPSDERLAKLVTGPEPTPKPAEPQVIQPQ
                     SPPEVTQPTNPAGPKGLWARLRGRWGKSE"
     gene            1640..2686
                     /locus_tag="PKL17_13085"
     CDS             1640..2686
                     /locus_tag="PKL17_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45716.1"
                     /translation="MGHAHLGTGTGAGARSRAEHRERAVAIVVERNRNTGKRLARLLV
                     SAGYTVKTYEDENTAIAPILHSDPQVGLWLVVGEAVAAAAIAEGLKTPPAGKRCVGVV
                     YGAGDEVDLALMFEQPAIAAVLGVKPSSGRPDLEAELLNVAAFLRGQPLLPVQGFLLW
                     GAQAFSASITNIGGRDAAESRLVKLCNEHLNISRRVADSLAEVVHELITNAMYDAPVD
                     AHGRTRYAHDRTANIELPAEDKVIFRYGTDGLRLAVEVVDRFGRLKRSDLVRSLRRAQ
                     SGQVNRGQGGAGIGLSLVCRTCPIVQVDVEPNCRTRLTAVIDLEPGRASEGPKPGRSV
                     IFPDLTAHAVRNDS"
     gene            2701..5136
                     /locus_tag="PKL17_13090"
     CDS             2701..5136
                     /locus_tag="PKL17_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNK45717.1"
                     /translation="MNYEERSGMIGAVQPPPKLLIERLSDGDVTCLRLMGTIDEQFDA
                     PGLAQTITCRYLILDLGGVDRISSFGIRQWIDFVQHIAPRLAGVYYVECSPKVVDQFN
                     MVANFGGAGYIVSFYAPYRCETCDQERRHLFRSDEETPVWRAGQAPATTCQTCSNKED
                     FDENPAMYFNYVAQQPIQAIPPAVMQFLRVHLNYGQGGQRKLRIEKRIEGRCTYIKLS
                     GDLDSDLKPEKLADGLEGEVVVDLGGILSIDPVGTAHWRKLMRTLDAKTGTDESVERV
                     SFIAVPAVFLERLGKPEDLTRKGQVLSLIIPYNCSRCRATTPKLVDFTMHGDELRAGR
                     VPRIKCALCGGPVTCVVSETWFNRLQTLPQPQVTPDLKSTIARLTAAPAPQPARSTMA
                     GPAMPAPTGAPAASAPSWAPQPMSGPPAQPQAPSWTPQPTPSPLATSGPRSTMGSSMA
                     QSSAAFPRPDLRAASQGTHATPSAPQPPPPQGVFGKLQNIPGLLPGLLVVMLSLSGAI
                     VYRILATPAGRAGEWKVVESSTPKAPLWHDPKFVSDGSKFVGHSTQVADRNDALERAD
                     IAAQAELISKLAEELETKDPDWRKVVKPLFSPVQDALKEELDKAQKSVKPTDDQAAAE
                     AQLQPARDHQFELQRRSTAAFKQSFGDGSPPALSQYWEKRTRTLKGAREAGFAASSLI
                     ELDKAAFDKLVNLFRQTEAAAKVKVMPFFPLLALRFESSEHGPVVIEVEDGSPLAQAG
                     LKSGDLVLSVLGKPVRTPGDFAKQIDLAVKDLASHDVVLKVQRGKELVDVKLTQAKTA
                     PKKGPVPIKKGGK"
     gene            complement(5166..7136)
                     /locus_tag="PKL17_13095"
     CDS             complement(5166..7136)
                     /locus_tag="PKL17_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK45718.1"
                     /translation="MSRPQLSQAAWLSAVALVGCVPASPEPAPIAQTVSPLSSDVLAL
                     FDKNDRPTDSFRVVGSLRPPSWSWGEIELLEGNQRVRTEGYNRRTEKLRAQDTFQSSD
                     YPLGTYFGSLNQQIVDGFNLFYKDSCATTKGATCSEPGPMRPEARFVLLHKGPKTYAR
                     TCSIDKTPLLLVHGAMQDANVWLFPGGNDGSGNAYAGTTQKTGMVQAMEAAGRCVYAV
                     TFGNFHGDNYSQAIAVANAIARIRKLHTKADGSAPKVDVLAWSKGVLAVDAYLANAAT
                     WPSLSSRFFDRVAAAQSEAVPAFRSDVRVYVALSGPHQGIDLNFRHPIHTLTIASTSW
                     NAPVGRGPMPWTWFSALQCVNWGPDVPWYSNPYAKSVCESRGGTWLDFFNRIYVSNVS
                     SLDSDGKPVSRGSLRSLNLTDGASSSSYSFDEYNISLFGSVNESGKWVSAYLGQLQAA
                     ADLRSDYPVPRRDSSDWKDLDADENRYYPWLRPKLSYLAGGYLDDPNRQACRKTAFAP
                     SASPCIAWHTAYNSRNADSSSGLYNKYQLMSGLGIASAVEMGGHFIDRLTAASLDSKL
                     DALYVLYGEAGGVAGSVYETDGMSCPTCAVNGDGVLFRKSIAAKDSLTSKWTSAKRTS
                     DAKQEGVSFGHLEVGVSPAVWTKVLTYLTARD"
     gene            complement(7176..8081)
                     /locus_tag="PKL17_13100"
     CDS             complement(7176..8081)
                     /locus_tag="PKL17_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45719.1"
                     /translation="MSSPRNRFRLGGHNLAQLAFGLVCVLCTGTSAHAQSGATEKPRR
                     VSLSYFGESAVNPGVRLAYDGTAYVSGANQLFMTASIGGYSTSPGYALFIFLGGGYRL
                     ALSSGLFLDLQIGIGYNALNRPGMNQMTPDGGVVQSAPTVGNYFMPIGLGGLGFDFQP
                     RMKVPVALFARGGGYGLTGTGEPFQGSYLLDVGLAYQFGTGKPTPVQSPVPSPPPAEA
                     PANLESQPDPNRVPLVIPPDGQPPVPPLVGPNGPTMPAGPIAPIAPAAPPAAPSLFPP
                     GSPSAAPGMTQPPTVPALPPPPASN"
     gene            8303..10612
                     /locus_tag="PKL17_13105"
     CDS             8303..10612
                     /locus_tag="PKL17_13105"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45720.1"
                     /translation="MSSQWPIRKRWTLLPLGVAALSMAPSCGPTGTEDAQQKAIIVTR
                     SGTGLGKVATAGGEISCGTDCSRLYAPGTSVTLTATADQASTFVGWSGACTGTSPTCD
                     IKLSTDPNEKTVTVDAKFDTQYVPPAVTLTVAKSGLGTGKVSSTDGAVNCGTTCQAQV
                     RIPSTVTLVAKADIGSGFAGWTGACDTQGTDPVCNVEVNLATTVTAKFDKRLCTPDNI
                     CWENPLPAGVNFSSVWGKSKTDIWAVGDSGTVLHYDGVAWSQTSSGVTQKLNAVFGFA
                     ANDVWAAGNGGTVIRWDGTKWSSVNSGTTNNLRGLWGTANNNLYFAGQAATLRKWDGT
                     AFSAITVPAAVAGRDLNAIHGLAANNIRVTGELGTLIRWNGTAWTVENSGSPRNQYAV
                     FNFDATHAWVGGYLGNTSIWDGTTWTWKPQSTGVTFNSIWGSGFEDVWFAGQAGLFFR
                     YYRDVKNNLVWEAKQSPVVSSLNGVFGFAANDVWAVGDSGAIIHYDGTAWTAGRSGLQ
                     AGWNGVWATGDSNIWAVGGSGTIAHWDGGAFSKVDSGVTDALYGVAGSSASSAWAVGE
                     NGTILRWNGSKWAASPSPTPVNLYAVAAPAAQDAWAVGAGGKALHFTGSSWTTVNTPT
                     SASLNAIWAPSSSEVWAAGDGGTVLKWNGTMWTQQPGPSTMNVLGLWGSSSTNLWAVG
                     PTGVYKFDGTNWTKQTVPTAGPFVGVWGFASGTVYVVGNAGQVLRYDGTTWKQLQSGT
                     TGNLTRVYGASTASVFVFGDGGQVLRTGT"
     gene            10681..14598
                     /locus_tag="PKL17_13110"
     CDS             10681..14598
                     /locus_tag="PKL17_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNK45721.1"
                     /translation="MRKNWFGSRIFLGAAVSLIGACGMPSAPEAPVLPTSNDGPAMRV
                     VTQVMHRDGESGQPTMARLHADTLTPMGSEVKSVAWSTLRGLKHNYNLSDEAVQGAKL
                     QDLHDAGNGPLIAHFDQEIDGIPVFRRSIKIAMDREMNPQFVTGNFAPSTSRVAERWS
                     LDETVAVSAAFRAMTGSRTAISRISFSGKQAGAYTALELESMPDSEGLSQNGPARVRK
                     VWYPTSEGLIPAYYTELDVGFNNSKDSRMMAFVVSAIDGTVFFRHNLTAADSFSYRVW
                     ADTTPPYRVWDSPMGNDYTPHPTGTRTSAAPGFKAANLVTLSSAGTSRNDPWLPDDAT
                     ELYGNNVFAYADLASPDGWGPGDAKVPLTGAKTFDRVYDPSTDPLASTNNSQAITTNL
                     FFILNWMHDYFYDAGWTESAFNPQKSNKGRGGIEGDPIRGEAQDSGGRNNANASTPAD
                     GGMPRIQMYVWDAVGASGKVNSPADVAGDYEVSGAPYGANPFSTTGELVIADDGVGNT
                     ADGCEAPFKNAAAMAGKIALMTFGEGNCASRVKVKNAQLAGAKGAIVVSVDPTFGLPA
                     MGGTDATITIGVIGMTYDDGMKLWNKVKGGATVNATIAGGRAVRDGSLEAGVVSHEWG
                     HIMSNRLIGNAVGLSNNQGGSMGEGWSDFVVLLSIVRPEDASVPANANWNGVFPDMAY
                     SVWAPGNKAFYDGLRRYPYSADLTKNPLTFKHIEDGVPLPTTPAPAFGGDGGSNAEVH
                     NAGEVWASVLWECYSSLLRDSRLTFDQANERMRRYLVSSLKLTPNAPTFLEARDAVLA
                     AAWVSDRADYDAFVKAFAKRGMGAGAQGPEKSSTDHAGVVESFVTGADVAIDRIVLDQ
                     SKTDCDQDGVLDNDEEGRLLVTIRNTGNKDLTLGTLKVTSSKPEVSVTTTNPATIPTL
                     APFQSTTVSVGVAMKGATGVTAFDLNLEVNGTGLYKPGAIKQTYSAQGNYDALPNQST
                     TDKFDSKNTGWSVVDDPQLYVEPWLYVYSAGNGMWHGPDSAEPSDHSFVSPELKIGAD
                     GIAITVKHRYSFETDGIGYYDGGVIELSDDGGTLWVDVTSVPGVAVSNGYTQSLAASP
                     SKNPLAGRRAFGGPSAGYPTDWVTTKIEIGKILAGKSVKLRFRTGSDDTVGAPGWDVD
                     EIAITGLANKPFGSQVPHRGVCVTKPASMFQIASTDQLAIPVGFQVQLVATATGATGA
                     VKWSWEQLDGPRVMLSATDIANPTFIAPAVSAPTQLSFKVKGTNGDVTRETIVNIQVV
                     KAQVTAENQGTGCNFGGAATGGSALGTLLVGAAAAMLRRRRRS"
     gene            complement(14749..15570)
                     /locus_tag="PKL17_13115"
     CDS             complement(14749..15570)
                     /locus_tag="PKL17_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNK45722.1"
                     /translation="MATQEDEKASSAPTSPKGVAAKADAQLDKLIGEDKQVSLRRRPI
                     DRQGLVAGLCLAIAINGGGLLLLWRASHKPDKPKASGPEIFVDVKLLKFGRKRDLSFL
                     PHVDATPRTADKNKIKLAQDPDKPSPKREDKKDDKLAKLTDLTKNLRSDDDDRATKEA
                     LEEGDPNGVRGGTASEAAGDPYLREIVAAVLERWTVPTMLKPEDLARLQAAACLTIAE
                     DGTLRAFRIVESSGNALFDGSLNATLGSIKTLPKPHGRFASAAKSGRLCPVFSKQ"
     gene            complement(15573..16013)
                     /locus_tag="PKL17_13120"
     CDS             complement(15573..16013)
                     /locus_tag="PKL17_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK45723.1"
                     /translation="MAMSGPKESTMVSEINVTPLVDVMLVLLIIFMVAAPIATTGVPV
                     VLPKGETQALDSEDAKIILTIPATFPKDQVVYLGKNPVRFAELELRLRTNEKLQSERE
                     MYIHADGSLRYQDVITVMSLASKAGADKVGLITDPMSSREESGQ"
BASE COUNT         3043 a         4906 c         5151 g         3028 t
ORIGIN      
        1 ggaatgggtg gcatgggcgg aatgggtggc atgggcggaa tgggtggcat gggcgacgac
       61 ttcggcagcg acttctagtc gacgccaagc tcgccggggc cggcaaggca gcttgccggt
      121 ttccctgtcc gctttttcct accgcgccaa cgccctgcct tggacaacgc tactcgcttt
      181 ttccccaccg tccccgcagc cgcgcccaca gccctttggg gccagcgggg tttgtcggct
      241 gcgtgacttc cggcggactc tgcggctgaa tcacctgcgg ctctgccggt tttggcgtgg
      301 gctctgggcc cgtcaccagc ttggccagtc gttcatccga cggacgattc gccgtgagct
      361 tgacgccgcc gggtccaaca gccacaccat cgatcgctgc cgctgtcatc acgagcgacg
      421 catcgcggtc gccatcgctt ggtagctcgg cgcgctggca agccgcgatc tctaccatct
      481 cttgatcgta gtcggcgcgg aacagcgact tgagctgctc gacgatttct ttgtccgaaa
      541 agcggtacgg cgccaaaaat gcttgaagat cctgcgccag ttccagcgcc gaggcatagc
      601 gatcggcgac atcccgtgcg agcgctttca tgacgatctt gtcgagcgct tcgggcacgc
      661 ggcggttgaa cttcgatggc ggcagaactt cggcgttgcg cacccgctcc atgagctgaa
      721 attcggtgtc gccacggaac agtttttccg tcgtcagcat ttcgtgcagg atgattcccg
      781 tcgaaaagac atcggtgcga gcatcgacag gcatgccccg gatttgctct ggggacatgt
      841 aactgaactt gccttttagc acgccgattt ccgacgtgaa cttcaccatg gcctgggcaa
      901 tgccaaagtc gagcagcttc acgctgccgt cccaacccag ccgaacgttc gatggcgaga
      961 cgtcgcggtt cacgatcgcc aacggtttgc catcggcatc gagcagcgtg tgggcgtgat
     1021 gaagaccctc gctcatcttg gcggcgacat aacaggcagc gggcagcgga atgcgcgagc
     1081 ccgtctcctg acagcggcgg aggatctggg ttagatcctt gccacctatg tactccatcg
     1141 cgatgaagta gcggccttcg gtgcgcccga tctcaaaggt gcgcacgatg ttgtcatggc
     1201 tgagcaagac ggccagcttg ccttcgcgaa cgaacatctc gacgaagcga gcttctttcg
     1261 caagctgcga tcgcatgcac ttgatcgcat acaggcgacc gccgctttgc cggctgcggg
     1321 cccgatagac ttcggccata cctccacggg cgatgaggcc caatagaaga tatttgccaa
     1381 agggctgggg attgtctgga gacccagggt cgagtttgtc ggctgcggaa tccattgcgc
     1441 ctattgtagc tttttggact ttatcagccc gtcggaatcg tgtctatcag cccggaatcg
     1501 gtgcttttgg accgattttg cgggtgggga cctgtacgca cgcagaacat gcgaagcagc
     1561 gtatcgacag accgtagcca agcactttac catgtggtct tgggcttttg tgcagcgcat
     1621 cacgaggaac acttctgcca tgggtcatgc acaccttgga acggggacgg gcgcaggcgc
     1681 gaggtctcgc gccgaacacc gagagcgggc ggtggccatc gtcgtcgagc gcaaccgcaa
     1741 cacgggcaag cggctggcca ggctccttgt gagcgctggt tacaccgtca agacgtatga
     1801 agacgaaaac accgccatcg cgccgatcct tcacagcgat ccgcaggtcg gactgtggct
     1861 ggtcgttggt gaagcggtag cggcggcggc gattgccgaa ggactgaaga caccgccagc
     1921 gggcaagcgc tgcgtgggcg ttgtctatgg cgcgggagat gaagtggatc tcgcgcttat
     1981 gttcgagcag cctgcgattg cagcggtgct gggggtcaag ccgtcgtcgg gacgcccgga
     2041 cctcgaagcg gagctgctca acgtcgcggc atttttgcgc ggccagccgc ttctgccggt
     2101 ccagggtttt ctgctctggg gcgcgcaggc gttttcggcc agcatcacca acatcggtgg
     2161 tcgcgatgcc gccgaatctc ggctggtgaa gctgtgcaac gaacacctca acatctcgcg
     2221 gcgcgtcgcc gacagcctgg ccgaagtggt ccatgagctg atcaccaatg cgatgtacga
     2281 cgcgccggtc gatgcacatg ggcgaacccg ctatgcgcac gatcggacgg caaacatcga
     2341 actgcccgcg gaagacaagg tgattttccg ctatggcacc gatgggctgc ggctcgcggt
     2401 cgaagtggtg gatcggtttg gacggctcaa gcggtcggac ctggtgcgtt cgctccgtcg
     2461 cgcgcagagt ggtcaggtca atcgcggcca gggcggggca ggcatcggtc tgtcgctggt
     2521 ctgtcggacc tgtccgatcg tccaagtcga tgtcgaaccc aactgccgca cgcggctgac
     2581 tgcggtcatc gatctagaac caggccgggc cagtgaaggt ccgaagcctg gtcgaagcgt
     2641 catcttcccg gacttgaccg cccatgcggt caggaacgat tcttaaggta ggctgccgtc
     2701 gtgaactacg aagaacgttc aggcatgata ggggctgtcc agccgccgcc caagctcctc
     2761 atcgagcgac tgtcggacgg cgacgtgacc tgcctgcgcc ttatggggac catcgacgaa
     2821 cagttcgacg cccctggcct ggcccagacc atcacgtgcc gctacctgat cctcgacttg
     2881 ggtggcgtgg accggatctc gtcgtttggt atccgtcagt ggatcgactt cgtccagcac
     2941 atcgcgccgc ggctggcggg ggtctactac gtcgagtgct cgccgaaagt cgtcgaccag
     3001 ttcaacatgg ttgcgaactt cggtggcgct ggatacatcg tttcgttcta cgccccttat
     3061 cgctgcgaga cgtgcgacca agaacgccgt cacctgttcc gcagcgacga agaaacgccg
     3121 gtctggcgcg cgggccaagc cccagcgacg acctgccaga cctgtagcaa caaagaagac
     3181 ttcgacgaaa acccggcgat gtattttaac tacgtcgccc agcagccgat ccaggcgatt
     3241 ccgccggcgg tgatgcagtt tctgcgcgtc cacctaaact acggtcaggg cggtcagcgc
     3301 aagctgcgca tcgaaaagcg gatcgaaggt cgctgtacgt acatcaagct gtccggcgat
     3361 ctcgatagcg atctcaagcc cgaaaagctc gcggatggac ttgaaggcga ggtggtcgtc
     3421 gatctgggcg gcatcttgtc gattgatccc gtcggtacag cccactggcg gaagctgatg
     3481 cggacgcttg atgcgaaaac cggcaccgat gaatcggtcg aacgggtcag cttcatcgca
     3541 gttccggcgg tgttcctcga gcgacttggc aaacccgagg atctgacgcg caaaggtcag
     3601 gttctgtcgc tgatcattcc gtacaactgc tcgcgctgcc gggccacgac gcccaagctg
     3661 gtcgatttta cgatgcacgg cgacgagctg cgggccggtc gggtgccgcg gatcaagtgc
     3721 gcgctgtgtg gcgggcccgt cacctgcgtc gtttcggaga cttggttcaa tcggcttcag
     3781 acgctgccac aaccgcaagt gacgccggat ctcaagtcga cgattgcgcg actgacggca
     3841 gccccggccc cgcaacctgc gcgatcgacg atggcaggtc cagcaatgcc cgccccgacg
     3901 ggagcaccgg ctgcttctgc gccaagctgg gctccgcagc cgatgtctgg tcccccggcg
     3961 caaccacaag caccaagctg gacgccgcag ccgacgccat cgccgctggc cacaagtggt
     4021 ccgcgatcga cgatgggttc gtccatggcg cagtcgagcg cggcttttcc gcgtcccgat
     4081 ctgcgcgccg caagccaagg gactcacgct acaccatcgg caccgcaacc accgccgcca
     4141 cagggcgtgt ttggcaagct acagaacatt ccggggcttt tgccgggcct gttggtggtc
     4201 atgctgtcgc tgtcaggcgc gatcgtgtac cgaattttgg cgacgccggc tggacgcgcg
     4261 ggcgaatgga aagtcgtcga gtcgtcgacg cccaaagcgc cactttggca tgatcccaag
     4321 tttgtgagcg acggcagcaa gttcgtcggt cactcgaccc aggtggctga tcgaaacgat
     4381 gcgctcgaac gggccgacat cgctgcgcaa gcggaactta tcagcaagct cgccgaggaa
     4441 ctggagacca aagatcccga ctggcgcaaa gtggtaaagc cgctgttttc gccggtgcaa
     4501 gatgcgctca aagaggagct cgacaaggcg caaaagagcg tcaagccgac cgatgatcag
     4561 gcggcggcag aggctcagct ccagccggct cgcgatcacc agtttgagct tcagcggcgc
     4621 agcaccgcgg cgtttaagca aagctttggc gatggctcgc cgccggcact ttcgcagtac
     4681 tgggaaaagc ggacgcgcac gctcaaaggc gcacgggaag ccggatttgc agcgtcgtcg
     4741 ctgattgagc tggacaaagc cgcgttcgac aagcttgtga acctgttccg gcagacggaa
     4801 gccgcggcca aggtcaaggt gatgccgttc ttcccactgt tggcgctgcg ctttgaatcg
     4861 tcggaacacg gacccgtcgt catcgaagtc gaagacggca gtccgcttgc ccaagctggt
     4921 ctaaagagcg gcgatctggt gctgtcggtg ctcgggaagc cagtgcggac gccgggcgat
     4981 tttgccaaac agatcgatct ggcggtcaaa gacctcgcca gccacgacgt cgtgctgaaa
     5041 gtgcagcgcg gcaaagagct ggtcgatgtg aagctgacgc aagcgaagac ggcgcccaaa
     5101 aaaggccccg tgccgatcaa gaagggtggc aagtagccgg actcgtcagc gacgagccgg
     5161 tgtcgctaat cgcgagccgt caggtaggtg aggactttgg tccaaaccgc cggggagacg
     5221 ccgacttcga ggtggccaaa gcttacgcct tcttgtttgg cgtcgcttgt gcgcttggcg
     5281 cttgtccact tgctggtcag gctgtctttt gcggcgatgc tcttgcggaa caggacgccg
     5341 tcgccgttga ccgcgcaggt cgggcagctc atgccgtcgg tttcgtagac ggaaccggcg
     5401 actccacccg cttctccgta caggacatac agcgcgtcaa gcttcgagtc gagactggcg
     5461 gctgtaagtc ggtcgatgaa gtgaccgccc atctcgacgg cactggcgat gccgagccca
     5521 gacatgagct ggtatttgtt gtaaagcccc gacgagctat cggcattgcg cgagttgtac
     5581 gccgtgtgcc aagcaatgca cggcgacgcc gacggtgcaa acgcggtctt gcgacacgcc
     5641 tgtcggttcg gatcgtccag ataaccgcct gccaggtagc ttagcttggg ccgcagccac
     5701 ggatagtagc ggttttcatc ggcatcaagg tctttccagt cacttgagtc gcgacgagga
     5761 accgggtagt cgctgcgcag atcggcggcg gcttgtagct gacccaaata ggcgctgacc
     5821 cacttgccgg attcattgac gctgccaaac aagctgatgt tgtactcgtc gaacgagtag
     5881 ctgctcgatg atgcaccgtc ggtcaggttg agcgatcgca gcgagccgcg cgatacgggt
     5941 tttccatcgc tgtcgagact gctcacgttc gatacataga tgcggttgaa gaagtcgagc
     6001 caggtgccgc cgcggctctc gcagacgctt ttggcatagg ggttactgta ccagggcacg
     6061 tcggggcccc agttgacgca ctggagcgcc gaaaaccacg tccacggcat gggtccgcgt
     6121 ccgaccggcg cattccacga tgtgctggcg atggtcaatg tgtggatcgg gtggcggaag
     6181 ttcagatcaa tgccttggtg tggtcccgaa agcgccacat agacgcggac atcgctgcgg
     6241 aacgcgggaa ccgcttcgct ttgggcggcg gcgacgcgat cgaaaaaccg gctcgaaagg
     6301 ctcggccagg ttgcggcatt cgcgaggtac gcatcgacag ccaagacgcc tttgctccag
     6361 gcgaggacgt cgactttggg ggcgctgcca tcggctttgg tgtgaagctt gcgaatccgt
     6421 gcaatggcgt tggcgacggc aatcgcctgg ctgtagttgt cgccgtggaa gttgccgaac
     6481 gtcaccgcgt agacacagcg tcccgccgct tccatcgcct gcaccatgcc ggttttttgc
     6541 gtcgttcccg cgtaggcatt tcccgaaccg tcattgccgc cggggaacag ccagacattg
     6601 gcgtcctgca tcgcgccgtg caccagcaaa agcggcgtct tgtcgatgct gcaagtgcgg
     6661 gcgtaggtct ttggtccttt gtgcaaaagc acaaagcgcg cttcgggtcg catcgggccc
     6721 ggctcactgc acgtcgcgcc cttggtcgtc gcacacgagt ctttgtaaaa caggttgaag
     6781 ccgtcgacga tctgctggtt taacgaacca aaatacgtgc cgagcggata gtcgctgctc
     6841 tggaaggtgt cttgggcgcg cagcttttcg gtccggcggt tgtagccttc ggtgcggacg
     6901 cgctggttgc cttccaatag ctcgatttca ccccacgacc agctcggtgg acgcaggctg
     6961 ccgacgacgc gaaagctgtc ggttgggcgg tcgtttttgt caaacagggc aagcacatcg
     7021 ctgctgagcg gagaaaccgt ctgggcaatc ggtgcgggtt ccggcgaagc gggtacgcag
     7081 ccgacgagcg caacagccga aagccatgca gcttgggaca attggggacg ggacatcgct
     7141 tccctcctat gtggtgtggc ggaagaaggc gtcgattagt tcgacgcggg tggcggtggt
     7201 aacgcgggca ccgtcggggg ctgcgtcatc ccaggtgctg cgctgggcga tcctggggga
     7261 aacagagacg gagcagccgg aggcgcagct ggcgcaatgg gcgcaatcgg acctgccggc
     7321 atcgtgggac cattgggtcc gacaagcggt ggcactggcg gctgcccgtc gggcggaatc
     7381 accagcggca cccggtttgg atcgggctga ctttccaggt tggctggcgc ttccgctggg
     7441 ggcggtgacg gaactgggct ttgtaccggc gtcggttttc cagttccaaa ctgatacgcc
     7501 aagccgacat cgagaagata actgccctga aatggctcgc ccgtgcccgt cagtccatag
     7561 ccgccgccgc gagcaaagag agccactggc actttcatcc ggggctgaaa gtcgaaaccc
     7621 agaccgccga gcccaatcgg cataaagtag tttcccaccg tcggcgctga ctgcaccacg
     7681 ccaccgtcgg gtgtcatctg attcatcccc ggacgattga gcgcgttata gccaatcccg
     7741 atctgcaaat cgagaaacag ccccgacgaa agtgccagtc gataaccgcc acccaggaag
     7801 atgaacagcg catagcccgg cgacgtcgag taaccaccga tgctggcggt cataaacagc
     7861 tggtttgcgc cgctcacata cgctgttccg tcgtaggcca agcgaacccc aggatttaca
     7921 gcgctttcac cgaaatacga cagcgagacc cgacggggtt tttccgtcgc gccggactgt
     7981 gcatgggcgc ttgtgcctgt gcacagcacg caaacaagcc caaacgcaag ctgggcgaga
     8041 ttgtgaccgc ccaaacgaaa tcggtttctt ggcgacgaca tgggttctac ctgggaccgt
     8101 agtcgcgttc gtccgcttgt gcaagtagct gcgcccctgt cccacgcaag cgtacaaaaa
     8161 aaccattgca cctacctgta acccaagggc taataagggg tgtgatacgg cgtgtcctgg
     8221 cccaagctct ttgcgaacgg tttttgtcgt cgaggtcagg accgactgca accggaaacg
     8281 agtgaaagaa agggtgctgt acatgtcctc tcaatggccg atccgtaagc gctggaccct
     8341 gctgccgctg ggcgtagcgg cgctgtccat ggctccctcg tgcggaccga cgggaaccga
     8401 agacgcgcag cagaaagcca tcatcgtgac ccgatccggt accggcttgg gcaaagtcgc
     8461 gacggcaggc ggagaaattt cctgcggaac cgactgctct cgtctgtatg caccggggac
     8521 gagcgtcacg ctcaccgcga cggcggacca ggcttcgacg ttcgtgggct ggagcggtgc
     8581 ttgtacgggg acgagcccga cctgcgacat caagctttcg acggacccaa acgagaagac
     8641 ggtcacggtc gatgcgaagt tcgataccca gtatgttccg ccggcggtaa cgctgaccgt
     8701 tgcgaagtca ggtctgggca ccggcaaggt ttcatcgacg gacggcgcgg taaactgcgg
     8761 caccacctgc caagcgcagg ttcggattcc atcgacggtg acgttggtcg cgaaggccga
     8821 tatcggatcg ggctttgcgg gctggaccgg cgcttgcgat acgcaaggca ccgatccggt
     8881 gtgcaatgtc gaagtcaatc tggcaacgac cgtcactgcg aagttcgata agcggctttg
     8941 cacgcccgac aacatctgct gggaaaaccc gctgcccgcc ggcgtgaact ttagttcggt
     9001 ctggggcaag tcgaagacgg acatctgggc cgtcggtgac agtggcacgg ttctgcatta
     9061 tgacggcgtt gcgtggtcgc agacctcaag cggcgtgacg cagaagctaa acgcggtgtt
     9121 tggctttgcc gcaaacgacg tgtgggcagc cggcaacggc gggactgtga tccgctggga
     9181 tggcaccaag tggtcgtcgg taaactctgg caccaccaac aacctgcgtg ggctgtgggg
     9241 aaccgcgaac aacaacctgt actttgccgg tcaagccgcg acgctgcgca agtgggacgg
     9301 cacggcgttt tcggcgatca cggttccagc agcagtcgct ggccgcgatc taaacgccat
     9361 ccacggtctt gcggcgaaca acatccgcgt caccggcgag cttggcaccc tgattcgctg
     9421 gaacggcacc gcttggaccg tcgaaaacag cggctcaccg cgcaaccagt acgcggtctt
     9481 taactttgac gcgacccacg catgggtcgg tggctacctg ggcaacacca gcatctggga
     9541 cgggaccacc tggacgtgga aaccgcaatc gactggcgtg accttcaact cgatctgggg
     9601 gtcgggcttc gaggacgtct ggttcgccgg gcaagccggt ctgttcttcc gctactaccg
     9661 cgatgtgaag aacaacctgg tgtgggaagc caagcagtcg cccgtcgtca gctcgctcaa
     9721 cggggtgttt ggattcgcgg ccaacgatgt gtgggcagtc ggcgactctg gcgccatcat
     9781 ccactacgac ggcactgcat ggacggcggg tcgcagcggt cttcaggctg gctggaacgg
     9841 cgtctgggca acgggcgact cgaacatctg ggcagtcggc ggaagcggca ccatcgcgca
     9901 ctgggacggc ggcgctttct cgaaagtcga cagcggcgtg accgatgcgc tgtacggcgt
     9961 cgcgggatcg tcagcgagca gtgcatgggc agtcggtgaa aacggcacca tcttgcgctg
    10021 gaacggcagc aagtgggcgg cctcaccgag cccgacgcca gtcaacctgt acgctgtcgc
    10081 agccccggct gcacaagatg cgtgggcggt tggtgccggc ggcaaggcgc tgcactttac
    10141 cggttcgtca tggacgactg tcaacacgcc gacgagcgcg agcctcaatg caatttgggc
    10201 gccttcttcg tcggaagtct gggcagcagg cgatggcggc acggttctca agtggaacgg
    10261 cacgatgtgg acgcagcagc ctggcccgag cacgatgaac gtgcttggac tgtggggcag
    10321 ctcgtcgacc aacctgtggg cagtgggccc caccggcgtc tacaagtttg acggcacaaa
    10381 ctggaccaaa cagaccgtgc cgaccgctgg tcctttcgtt ggtgtgtggg gcttcgcaag
    10441 cggcaccgtc tacgtcgttg gcaacgctgg ccaggtgctg cgttacgacg gcacgacgtg
    10501 gaagcagctc cagagcggca ccacgggcaa cctgacccgc gtctacggcg ccagcactgc
    10561 gagcgtgttc gtgtttggcg acggcggcca ggtgctgcgc accggaacgt agcttggggc
    10621 agaccaactt ttaacgtggc gaggtggcgt gatggccacc tcgacagcta ggagcaaacg
    10681 atgcgaaaga attggttcgg atcgcgaatc ttcttgggtg ccgcagtcag cctgatcggg
    10741 gcctgcggta tgccaagtgc gcccgaagcc ccggtgctgc caaccagcaa cgatgggccg
    10801 gcgatgcgag tcgtcactca ggtgatgcac cgcgacggcg agagcgggca gccgacgatg
    10861 gctcgtctgc atgccgacac gcttacgccg atgggcagcg aagtgaagtc ggtggcgtgg
    10921 tcgacgctgc gtggactgaa gcataactac aacctgtccg acgaagcggt gcagggggcc
    10981 aagcttcagg atctgcacga cgctggcaac ggaccgctca tcgcgcactt tgatcaggaa
    11041 atcgatggca tccccgtctt ccgtcgcagc atcaagattg cgatggatcg ggagatgaac
    11101 ccgcagtttg tcaccggcaa cttcgccccg agcacgagcc gggtggcgga gcgctggtcg
    11161 ctcgatgaaa cggtcgctgt gtcggcggca ttccgggcga tgaccggcag ccgcaccgcg
    11221 atctcgcgga tcagcttttc cggcaagcaa gcgggggcct ataccgcgct cgaactggag
    11281 agcatgccgg attccgaagg gctgtcgcaa aacggcccgg cgcgcgtccg caaggtctgg
    11341 tatccgacga gcgagggcct gatcccggcc tactacaccg agctagacgt cggctttaac
    11401 aacagcaaag actcgcggat gatggcgttt gtcgtctcgg cgatcgacgg cacggtgttc
    11461 tttcggcaca acctgaccgc cgctgattcg ttcagctatc gcgtctgggc tgacaccacg
    11521 ccgccgtacc gcgtgtggga cagcccgatg ggcaacgact atacgccgca cccaacgggc
    11581 acgcgcacca gcgctgcgcc gggcttcaag gcggccaacc tggtcacgct gtcgagcgca
    11641 ggtacgtcac gcaatgaccc gtggctgccc gacgatgcga ccgagctgta tggcaacaac
    11701 gtgtttgcct acgccgacct tgcttcgccc gatggctggg gccctggcga cgcgaaagtg
    11761 ccgctgactg gtgccaagac gtttgatcgc gtctacgacc cgtcgaccga tccgcttgct
    11821 tcgacgaaca actcgcaggc gatcacgacc aacctgttct tcatcctcaa ctggatgcac
    11881 gactacttct acgacgcggg ctggaccgaa tcggccttca atccgcagaa gtcgaacaag
    11941 ggtcgaggcg gcatcgaagg cgatccgatt cgcggcgagg cgcaggattc cggcggtcgc
    12001 aacaacgcca acgccagcac gcctgctgac ggtggcatgc cacgcatcca gatgtacgtg
    12061 tgggatgcgg tcggcgcgtc gggcaaggtc aattcgccag ccgacgtcgc gggcgactac
    12121 gaagtgtcgg gggcaccgta tggtgccaat ccgttttcga ccaccggcga gctggtgatt
    12181 gcggacgacg gcgtcggcaa caccgctgat ggctgcgaag cgccgtttaa gaatgcagcg
    12241 gcgatggccg gcaagattgc gctcatgacg ttcggcgaag gcaactgcgc ctcgcgcgtg
    12301 aaggtcaaaa atgcgcagct tgcaggtgcc aagggtgcga tcgtcgtcag cgtcgatccg
    12361 acgttcggac tgccggcgat gggtggtacg gacgcgacca tcaccatcgg cgttatcggc
    12421 atgacctacg acgacggcat gaagctgtgg aacaaggtca aaggcggtgc caccgtcaat
    12481 gccaccatcg cgggcggtcg cgccgttcgt gatggctcgc tcgaagcagg tgtcgtctcg
    12541 cacgagtggg gccacatcat gtcgaaccgg ctgattggca acgccgtcgg tctgtcgaac
    12601 aaccagggcg gcagcatggg cgagggctgg agcgacttcg tcgttcttct ctcgatcgtt
    12661 cgtcccgagg atgcaagcgt cccggccaat gccaactgga acggcgtctt tccggacatg
    12721 gcgtattcgg tgtgggcacc cggcaacaag gcgttttacg acggcctgcg tcgctacccg
    12781 tactcggcgg acctgaccaa gaacccgctg acgttcaagc acatcgaaga cggtgtgccg
    12841 ctgccgacga ccccggcgcc cgcctttggt ggcgacggtg gaagcaatgc cgaagtccac
    12901 aacgccggcg aagtctgggc atcggtgctg tgggaatgct attcgtcgct gctgcgggat
    12961 tctcgtctga cgttcgacca ggccaacgag cgcatgcgcc gttacctcgt gtcatcgctc
    13021 aagctgacgc cgaatgcacc gacgttcctc gaagcgcgtg atgcagtgct ggccgctgcg
    13081 tgggtgagcg accgcgccga ctacgatgcg tttgtgaagg cgttcgccaa gcgtggcatg
    13141 ggcgccggcg cgcaaggtcc agagaagagc tcgaccgatc acgcgggtgt ggtggagagc
    13201 ttcgtcacgg gtgccgatgt ggcgatcgat cgcatcgtgc tcgatcagtc gaagaccgac
    13261 tgcgaccagg acggcgtgct cgacaacgac gaagaaggcc gcctgctcgt caccattcgc
    13321 aacaccggga acaaggatct gaccttgggc acgctcaagg tgacgtcgag caagccggaa
    13381 gtttcggtca cgacgacgaa cccggcgacc atcccgacgc tggctccgtt ccagtccacc
    13441 accgtcagcg tgggcgtggc gatgaagggc gcaaccggcg tgaccgcgtt tgacctcaat
    13501 ctcgaggtaa acggcacggg cctgtacaag ccgggcgcga tcaagcagac ctattcggcg
    13561 cagggcaact acgacgcgct cccgaaccag agcaccaccg acaagttcga cagcaagaac
    13621 accggctggt cagtcgttga tgatccgcag ctttacgtcg agccatggct gtacgtctac
    13681 tcggcgggca acggcatgtg gcacggtcca gacagtgcgg aaccttccga tcactcgttt
    13741 gtttcgcctg agctgaagat cggcgccgat ggcatcgcca tcaccgtgaa gcaccgctac
    13801 tcgttcgaga ccgacggcat tggctactac gacggcggcg tgatcgagct gtccgatgat
    13861 ggcggcaccc tgtgggtcga cgtcacgtcg gttccgggtg tggcggtgag caatggctac
    13921 acccagtcgc ttgcggcctc accgtcgaaa aacccgctcg ctggtcgtcg cgcgttcggt
    13981 ggtccaagcg ctggctatcc aaccgattgg gtcacgacca agatcgagat tggcaagatc
    14041 ctcgccggca agtcggtgaa gctccgcttc cgcaccggca gcgatgacac cgtcggcgca
    14101 ccgggctggg atgtggacga gattgccatc actggcctcg ccaacaagcc gtttggttcg
    14161 caggttccgc accgtggcgt ctgcgtgacc aagccagcgt cgatgttcca aattgcatcg
    14221 accgaccagc ttgcgattcc ggttggcttc caggttcagc tcgtcgccac cgcgaccggc
    14281 gcaactggcg cagtcaagtg gagctgggaa cagctcgacg gtccgcgcgt catgctgtcg
    14341 gccaccgaca ttgcgaaccc gaccttcatt gcgcctgcgg tgagtgctcc gacccagctt
    14401 agcttcaagg tcaagggcac caacggcgac gtgacccgtg agacgatcgt caacatccag
    14461 gtcgtcaagg cgcaggtcac ggcggaaaac caaggcacgg gctgcaactt cggcggcgcg
    14521 gccacgggcg gctcggccct gggtacgctg ctcgtcggtg ctgcggctgc gatgctccgt
    14581 cgtcgtcgcc gtagctaacc cacctgcctg cggggggcct cgtgccccct cacttatccc
    14641 ccacaagtcc gctttttcgc cgcaaagaga ctcgctctct ttgacggcgt agctgcgtcc
    14701 atcgacccgc ttaccgtcgg atcggcacag cgcgcagcga ctccattgtc actgcttcga
    14761 aaacaccgga cagagccgac cgcttttggc ggcagaagcg aaccgtccgt gcggcttcgg
    14821 cagcgtcttt atgctaccga gcgttgcatt caggctgccg tcaaacagtg cattgcccga
    14881 cgactcgacg atgcggaacg cgcgcaaggt gccatcttcg gcaatcgtca gacacgccgc
    14941 cgcttgcagt cgcgccagat cctcgggctt tagcatcgtc gggaccgtcc agcgctccag
    15001 cactgcggcg acaatctcac gcaggtacgg atcgcccgct gcctcgctgg ccgtaccgcc
    15061 gcgcacgccg tttggatcgc cttcttccag cgcttctttg gtcgcccggt catcgtcatc
    15121 gctgcggagg tttttcgtca ggtcggtcag cttcgccagc ttgtcgtcct ttttgtcctc
    15181 acgctttgga ctgggcttgt ccgggtcctg cgccagcttg atcttgtttt tgtcggcggt
    15241 gcgcggtgtc gcatcgacgt gcggcagaaa cgacaggtcg cgcttgcggc caaacttgag
    15301 cagcttcacg tcgacgaata tctcgggtcc gctcgctttc ggcttgtcgg gcttgtgact
    15361 ggctcgccac aaaagcagca gcccaccgcc gttgattgca atggccaggc acaggcccgc
    15421 gacgagcccc tgcctgtcga tcggtcgtcg ccgcagcgag acttgcttgt cctcgccgat
    15481 cagcttgtcg agctgggcgt cggctttggc cgccactccc ttggggcttg tcggcgccga
    15541 agaggccttt tcgtcctctt gtgtggccat gactactgcc ccgactcctc tcggctcgac
    15601 atcgggtcag tgataagacc cactttgtcg gcccccgctt tcgacgccag cgacatcacc
    15661 gtgatgacat cctggtagcg caagctgccg tcggcatgga tgtacatctc gcgctcgctc
    15721 tggagctttt cgttggtccg cagccgaagt tccagttcag caaagcgcac cgggttttta
    15781 cccagataga ccacctgatc tttgggaaac gtcgccggga tcgtcaggat gatcttcgcg
    15841 tcttcactgt cgagcgcctg ggtttcccct ttgggcagca ccaccggcac tcccgtcgtc
    15901 gcaatcggtg ccgccaccat aaagatgatc agcagcacca gcatcacgtc gaccagtggc
    15961 gtgacgttga tctcagagac catcgtgctc tctttggggc cagacatcgc catgcgcggg
    16021 agtatagcgt ttttctcgtc gcgataccca actagagacg gcagcgaacg aggagggcaa
    16081 gggtaggtcg tcggattaca tgtggtgtgc tagcgtaaca gggctctt
//
LOCUS       DAORGN010000114        16104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1486_length_16104_cov_5.687333, whole genome shotgun sequence.
ACCESSION   DAORGN010000114 DAORGN010000000
VERSION     DAORGN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1486_length_16104_cov_5.687333"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKL17_13125"
     CDS             <1..615
                     /locus_tag="PKL17_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45724.1"
                     /translation="AIGLPKPAPPVAASVAVHRPLPSPSAAGTSPGPAATNPTVAVST
                     PATAPVPAVGTEKNPERSAERPAVLRAAPISAAERAFSEGWSALRKNDFSVAADAFGR
                     AEQGASGEPIVEDARFWRAVALGRAGQVQAETAALQRFLSLHGGSPRAGEAAAILGWL
                     LLNQGQTEAAAKHFANASRDPRDEVQKSARAGLAAVERQMKASR"
     gene            complement(612..1790)
                     /locus_tag="PKL17_13130"
     CDS             complement(612..1790)
                     /locus_tag="PKL17_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNK45725.1"
                     /translation="MGRKVYVIGVGMVKFQKPGASDSYDVMAQGAAKSALKDAGVDYG
                     DIQQVYAGYVFGDSTCGQRAVYPLGLTGIPVVNVNNNCSTGSTALFLARQAVMGGDAD
                     CVLALGFEQMEKGALGSKWTDRTNPMDKHVEVMNRQQGFNQAPPTAQMFGGAGREYRW
                     KYGTRRETFAKIASKARNHASRNPLALFNQPLTVEEVMASEEVFDPLTRFQCCPPTCG
                     AAAAVIASEDFVRRKGIAGAVRIVAQHMSTDFGSAFGDSMIGMIGYDMAKGCAAELYK
                     KAGIGPQDVQVVELHDCFTTNELLTYEAIGLCPEGGAEQFIWDGQNTYGGKYVTNPSG
                     GLLSKGHPLGATGLAQCAELTWQLRGQADKRQVEGAKVALQHNLGLGGACVMTMYARD
                     "
     gene            1982..3682
                     /locus_tag="PKL17_13135"
     CDS             1982..3682
                     /locus_tag="PKL17_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNK45726.1"
                     /translation="MTESKSSTLKARIAAFAGRLNKISAKVPPRRERFATDADIPISD
                     LYSPIDAPHTQADEAGGSSPYLDGIGLPGEYPFTRGVQPNMYRGRLWTMRQYAGFGSA
                     RDSNQRYHYLLSRGQTGLSVAFDLPTQMGRDPDHELSKGEVGRVGVSIASLADMRVLL
                     SGLPLEKITTSMTINATAATLLALYVAVADEQGVSRKQLGGTVQNDILKEYMARGTYI
                     YPPRPSLRLITDLFSFCNAELPQWNTISISGYHIREAGCDAVQEVAFTLADGIAYVEA
                     ALKVGLKVDEFAPRLSFFFNGHNNLIEEVAKFRAARRLWAKIMRERFSAKDEKSMMLR
                     FHCQTAGSMLTAQQPLTNVVRNSVQALSAILGGCQSLHVNSYDEALALPSEQSALLAL
                     RTQQVLAYESGVADIVDPLGGSYAVESLTDQIEAAAQSYIQRIDELGGMVAAIEQRYP
                     QREIERRAYEYQRQVESKQRIVVGVNDFVISEPPPKGLHHLDPQAEIDRVTEIRSFRT
                     ARDEQNTRQALRALDQKASRPSASDNNLTAAILDAVKAQATVGEIADTLRIVFGEYQG
                     "
     gene            3774..4565
                     /locus_tag="PKL17_13140"
     CDS             3774..4565
                     /locus_tag="PKL17_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45727.1"
                     /translation="MSDRRFSQGTLSFRRFWAGSLLALALSTTGCFGAHYRPSYSMQN
                     VQAKPASLMVAQKIQRPLFLVVDSAKVPDSWDLKESSSLNPKDGEQTKLIDFQQFVRR
                     DLKEALGNYFAKVEVVKAGTPLPAEPHVVADVKVDRVQLHSTPVGRLVYTIIEMTWGV
                     GLRPSEQAEYAFAFAGEGKSSESYPTFEVGVAQLVESAILGFNKSLVEKGGLEALQKA
                     SAPAAPQAAAAANPAGGAAAQAGGEPLDLAAPGKPASKAGKKRSK"
     gene            complement(4569..8660)
                     /locus_tag="PKL17_13145"
     CDS             complement(4569..8660)
                     /locus_tag="PKL17_13145"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK45728.1"
                     /translation="MNRLQAQPQIRRSLGAARLRRLFGLALLSLLGLSACTKIPPPQP
                     TVASVEASRDGAHIKRGNTTLPIVRNVRLEVGSEVTTPAGVRASLYLDAGAWVLLDQS
                     SQVTIRDKGIEVKSGRAWIDARVGNDVPVVAQGVELSAQRAGLAVTITDGGAKVYCAS
                     GQAAWKRGDKAGRLESGLTLLVSGEEAKQTAQALWDDFTLGLAEPGPLRALEPAGVGQ
                     LTARPTSGIGTARTPLIVRSHEVTVTIDGDLATTRVEQTFFNPRSEYLEGLYTVRLPE
                     SAILEHFSVAEDDRAPRRGVVLSMDPKYGGVSSFAANLEWAGAGRYRGLVRNIAPGKV
                     RRVVLLYREWLPHIPHPDAVKRTYVYPMGQSAQMQAGTLSNLGEFSLTVDVSKSGAAA
                     LQAGMGAQLENSQVTLRRSDFRPTADFVLDLIDGKDRISERLFGFRGEELDGQRYLLI
                     QPTPAPGKAPKGLDVVLLVDVSAGTDEAKLSLGRSVAQAVLKRLTPEDRVAVVAASLG
                     AATLSSEAMAPATKERIEQLDAQLAKQTPGGATNLQLSFQRAVELLPRGQGAVIYIGD
                     GRPTVGALTPRELREQLQRLGDLPRFFAAAVGTDANLPLLESLCHSGIRSGVVRIDDA
                     PDASRAALYLLERIAQPSLTNVTVDLGEGADVVYPDEPVTLESGDALTVVARLPKDRG
                     LPGKLQVRGVRDGQPFVEQRDLTVRTMDDGGDIARRWALLRMQGLLERASGKEAIVDI
                     GKRFQVVTPWSAIVVEGSGGETYRPLVGDLQAGQFVPPALRGAAPQDSSIVLESGVST
                     ANVRTISVNEMYVRALNGQSSAARLCYERRAAGNPGLSGRVELKVKLGLDGIPKDVRV
                     SSSTLRSKEIDACIERALLAVKLPASPDGKLHDIDYALQLNQPDRDDGPQRCSAASRA
                     YLQVRRSLWRERLASHPGVEGAMTVYREAAARCELKTWLDRRAIIDMLRVHVGATAQQ
                     VDLYHRFDDSENAHEIQSHLRREILRAVRTEADIRAAQSGLALDGGVDQTLLAQELKK
                     AKSVDDQLVVVRRFLLLAPDSLSLKLKLLALLEEASGEKGVSAERKQALFSEANRLIE
                     TTRSDPGADASVRQAVGEYLIRRGEGQEGARALSEIVEFAPFDPWGRRRLGDLYRAHG
                     FYDAAYREYQGLSWLLPQDEAVMLLLADAAAGLGRSDEALRLTSRVAEAVGAQKTQKG
                     APAWARALYAVRLSRLVDDAQKKGDQNLLGQLKVRGRSDGLAGYAGKLLIAVTWSHPD
                     AQLQLQLQPPGRSSELTRAALQGGSVGIEAQRFDRIDAGEWKLRVYKSGGDAASEYQG
                     ELTVIQNAGDEAEVILRKPISFSATSGSVRDFVLRGKELSPAK"
     gene            complement(8670..13361)
                     /locus_tag="PKL17_13150"
     CDS             complement(8670..13361)
                     /locus_tag="PKL17_13150"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK45729.1"
                     /translation="MRYAHRLPGHPWLAQLRKHTRLFALLCSALSLGLSACGPKLSAV
                     SSVASVEVVRTPVELAVSSSRHAISGEERASADQTITVAERAQAIIHHDVGARLLVDG
                     GSEVIVTDTGVKISRGRIFVDAKSGGRLEVGVGDVSLLASAAGFEVSKTTGPARLSVI
                     RGEVAYVGSGRGVVHSGEQLSVEANAAKLTPIALWDDWTGGLAWPDPRVGRGAPGLGE
                     VGARKPGSLGEARFPLSLNRLEVRVRIEDDLAITTVEETFFNPDESTLEGIFRVRLPE
                     DAILSRFAIDRRGRFVDGYVKERETARRDYESKVYEGSTHDPALLEWEAPGQYKARLY
                     PLPPGSTRRVLYTYSQWLPLAGLGNSLRTYRFPMGAAAGAGAPIIQELDIEVDVSHAG
                     TKEVRSGLGAVLSDDHKRVVLQRTDFVPSADLTVELVSDKRLDDVARVYHVPHQDPPR
                     PMGHSGKPEDEYVLIRTLPAPLPTATERAPMDLALLVDVSAATDATHLAMARTLIESV
                     LRRLGPDDRVVLLGADLDAHAVLGGKTGKKEKAAAPTWSTMSGEVANQLLEQLGRAKT
                     GGATDLGRTLSEAAAMLDPSRIGAVLYVGDGKPTVGELSLKNLRERLASLPQPLRLYS
                     VGIGDDANLDLLSGLSEVSAGQAYRVGDRASAAETALKVVSHLSRPTVSKLKFEVGSS
                     LDRVYPRETVAVRAGEPLLILARLKKRLPSTITVEGVQLGQPFKRTYRLEGHPIDNTS
                     DLRLRWSAARLSQLLESGASVEEVAELGVRQNLITPYTSYYVPSEEEVSALEQAIPEV
                     RAAGCSSRSPMSFAKSASEAAPAFMAPSSPTVVSADPSSSARNEEGAMGGRAGAAKRK
                     AAPNKDVDQGEAAKSAPADDEAPPAPPPAEPASAAAPAPKASRAMKEAVSEGGDYGGA
                     DRKAKRSASRDKADSLADALDGDAFGLVGSGKGGGGTGEGTIGLGSLGTIGHGGGSGY
                     GAKNRRSDLGADVIAGNAEVRGSLDKEVIRRVIRRHMNEVKFCQERELSRGHAGNGRI
                     VVQFTVGAEGSVLASTIQSSTVGNPVVDACIAGAIRRWEFPRPRGGIVVVSYPFILKS
                     NVNYPSAPDMESAPAVAISTPEVTVVVGILTHRPKGCSAASMQPLDERKQLWKERLSG
                     GVRKAMEVMQDAVSGCEVRRWSERRELLRLMLRNVGGPSGMVDLYNNLSDEPDDQEYL
                     RHAILGEVRSPRDLRVIYDGLGLGDEERAMLAQEVVAKAKSMPERLTRLEELLAKWPG
                     DLKLRIMRMETEESLGEADSARRTAEEIRQDPYADAQARTSVGELFVRLGDEIGARRA
                     FSEIVEFSPDDPLARRRLGDLYRAHGWFDEAYRQYETLLQLQPGDNSVLILLASAAAG
                     AGKVEEALGLEKRVVGDAEPGSESGLAKIALLWSSLRLAELREQARTQPGKDTAEELA
                     LLHLRARQNGIEGLYRPVRVFLTWAHPEADLDLRVGAPGESLGPAIDLAPQFGLLGWH
                     SHKDASSKGEYVVEVRRQDQKRAIGYDVVLTVVLSEGQKDEKLLSSKFKLAGELTERR
                     LLLRDGRLLPQ"
     gene            13575..15149
                     /locus_tag="PKL17_13155"
     CDS             13575..15149
                     /locus_tag="PKL17_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK45730.1"
                     /translation="MDCFAQTVDYAARGAVYFGKRIAVVDVERGDAGRFDYLSLSQRA
                     TRLARFLVEKGVQKGDRVGLLAQNGIEYIDLLLACAKLGAIFSPYNYRMHPRELLEVV
                     EKTRPRVFVYGPEYKAHAELLMGEQATISSVNTFLLAGLNPQLGQGTQSFEQVVTEAP
                     LDGAPLFSPGDGPGGEDILCLLGTGGTTGLPKAAMISHRMCAHNALTTMIHELQPGDV
                     TVIHTPMFHTGGLLVYALPLLFSGGKVVIMRKWDSEQLLSLIPREQVTMFFCVPTQYQ
                     MLLDSPSFAKTSFASVRFVTSGGAPLPIPLIAAFRSVHDIPFKQGFGMTEFGPGCFSM
                     GAEYAQSKAGSIGRPNAFVGARIVDESGVDVAVSEAGELLLKGPSMFSGYFEDDSATR
                     AAIDAQGWFHTGDIARSDSDGFFYIVDRKKDMFISGGENVYPVEIERALYEHPAVKQC
                     AVIGIPDEKWGQVGRAYVVLHAEPAGTPLPSEESLLDFLRSKLARYKVPKGVRFLSEL
                     PLSPAGKILKRALLEK"
     gene            complement(15222..15611)
                     /locus_tag="PKL17_13160"
     CDS             complement(15222..15611)
                     /locus_tag="PKL17_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45731.1"
                     /translation="MGLSAACTYALAPPLNYTSHGTPMYMAPELASGSKYARPAADLF
                     ALGVIAYELLAKEMPFSSPVVWAKRPSDLKVAVPFRSLGLALPDSLLGLLERCLHPDP
                     LMRPTAERLHTALAGLPPIAKQSRPDT"
     gene            15719..>16104
                     /locus_tag="PKL17_13165"
     CDS             15719..>16104
                     /locus_tag="PKL17_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNK45732.1"
                     /translation="MSLDVQRERIVAYCKMFQHNLIAIYADDHTGRNLERPGFQAALA
                     RMVETDSILMSVSMDRISRNVGDWTYLLDAYFGKGKSYKLLAFDCAGMDPRTATGRML
                     LMMRAVMAQGEIDSTSERTQVAMDHL"
BASE COUNT         2986 a         5044 c         4924 g         3150 t
ORIGIN      
        1 gccatcgggt tgcccaagcc ggcgccgcct gtcgcagcgt ccgtcgcagt ccatcgaccg
       61 ctgccatccc caagcgcggc gggcacaagc ccaggtccgg cggcaaccaa tccaaccgtc
      121 gctgtttcga cgccagccac tgcacctgtg cccgctgtgg ggaccgaaaa gaatcccgag
      181 cgaagcgcgg aacgaccggc ggtgcttcgt gcggcaccga taagcgctgc ggagcgcgca
      241 ttttctgaag gctggtcggc gctgcgcaaa aatgattttt ccgttgcagc cgatgccttt
      301 ggccgtgctg agcaaggcgc aagcggcgaa cccatcgtcg aagatgctcg tttttggcgg
      361 gcggttgcgc tgggtcgggc agggcaggtt caggcagaaa cggcggcgct tcagcgcttt
      421 ttgtcgctac acggcggctc gccgcgggcc ggggaagcgg cggcgatttt gggctggctg
      481 cttttgaatc agggacagac ggaagcggcg gcgaagcact ttgccaacgc cagccgcgat
      541 ccacgggatg aagtgcaaaa gagcgctcgc gccggacttg ccgccgtcga gcgacagatg
      601 aaagcgtcgc gttagtcgcg cgcgtacatc gtcatgacgc aggcgccgcc cagaccgagg
      661 ttgtgctgaa gcgcgacctt ggccccttcg acttggcgct tgtctgcctg gccacgcagc
      721 tgccaggtca gctcggcaca ctgcgcaaga cccgtcgctc cgagcgggtg gcctttggac
      781 aaaagcccgc ccgatggatt ggtgacatat ttgccgccgt aggtgttctg gccgtcccag
      841 ataaactgct cggcgccacc ttctggacac agtccaatcg cttcgtaggt caaaagttcg
      901 ttggtggtga agcagtcgtg cagctcgacg acctgaacgt cttgcgggcc gatgccggct
      961 tttttgtaaa gctcggcggc gcagcccttg gccatgtcgt agccaatcat gccgatcatg
     1021 ctgtcgccaa acgccgaacc aaagtcggtg ctcatgtgct gggcgacgat gcggaccgcg
     1081 ccggcgatgc ctttgcgacg cacgaaatct tccgaagcaa tgaccgcggc ggcggcgcca
     1141 cacgtcggtg ggcagcactg gaagcgggtc agcggatcga acacttcctc agatgccatc
     1201 acttcttcga cggtgagcgg ctgattgaac agggccagtg gattgcggct ggcgtggttt
     1261 cgggctttcg acgcgatttt ggcgaaggtt tcgcgacggg tgccgtattt ccagcggtat
     1321 tcgcgaccgg cgccgccgaa catctgcgcc gtcggtgggg cctggttaaa gccctgctgg
     1381 cggttcatca cctcgacgtg tttgtccatc ggattggtgc gatcggtcca cttgctgccg
     1441 agcgcgcctt tttccatctg ttcaaaaccg agtgcgagca cacagtcggc atcgcccccc
     1501 atcaccgctt gccgcgccag aaacagcgcc gtcgatccag tcgagcagtt gttgttgacg
     1561 ttgacgacag gaatgccggt aagtccaagt ggatacaccg cgcgctggcc acaggtgctg
     1621 tcgccgaaga cgtagccggc gtagacctgc tgaatgtcgc cgtaatcgac gccggcatct
     1681 ttgagcgcag acttggccgc gccttgtgcc atcacgtcgt aggagtcgct tgcgccgggt
     1741 ttttggaact tcaccatgcc gacgccaatg acgtaaacct tgcgacccat cgtgccgtac
     1801 tcctttcgca gaaagccgca accgcttggg ccgcagcgct ggggataaag tgaatctctc
     1861 cttgggtaca ccaaagccga gcctttgtca ccctgggtgc agaatgcgca acgactgcgc
     1921 cccggatggc agacatctgc tgttgcttgg gtcgcccgct gttttgtagc ctcatgccga
     1981 catgacagag tcgaagtcat cgacactcaa agcgcgcatt gcggcgtttg ccgggcgctt
     2041 aaacaagatt tccgcgaaag tgccaccgcg ccgcgagcgc tttgccaccg acgcagacat
     2101 tccgatttct gatctgtatt cgccgatcga tgcgccgcac acgcaggccg atgaagcggg
     2161 cggatcgagt ccctacctcg acggaattgg cctgcccggt gagtatccgt ttacgcgcgg
     2221 cgttcagccc aacatgtatc gcgggcggct gtggacgatg cggcagtatg cggggtttgg
     2281 ttcagcgcgc gactcgaatc agcgctatca ctacttgctt tcgcgcggac agacgggcct
     2341 gtcggtggcg tttgatctgc cgacgcagat gggccgcgac cccgaccacg agctgtcgaa
     2401 aggcgaagtc ggtcgcgttg gtgtgtcgat tgcgtctctt gcggacatgc gggtgctttt
     2461 gtcggggctg ccgcttgaaa aaatcaccac gtcgatgacc atcaatgcca ccgcggcgac
     2521 gctgctcgcg ctgtatgtgg cggtggccga cgaacaaggg gtgtcgcgca aacagctcgg
     2581 cggcaccgtg caaaacgaca tcttgaaaga atacatggcg cgcgggacgt acatttatcc
     2641 gccacgcccg tcgctgcggc ttatcaccga cctgttttcg ttttgcaatg ccgagctgcc
     2701 gcagtggaac accatcagca tcagcggcta tcacattcgc gaagcgggct gcgatgcggt
     2761 gcaggaagtc gcgtttacgc tcgcagatgg catcgcttac gtcgaagcgg cgctcaaagt
     2821 gggcctgaaa gtcgacgagt ttgcaccgcg gctgtcgttc tttttcaacg gccacaacaa
     2881 cctgatcgaa gaagtcgcca agttccgtgc ggcccgtcgg ctgtgggcca agatcatgcg
     2941 cgagcgtttt tccgccaaag acgaaaaaag catgatgctg cgctttcact gtcagacggc
     3001 gggatcgatg ctgacggcgc agcagccttt gaccaacgtg gtgcgcaaca gtgtgcaggc
     3061 gctgtcggcg attttgggcg gctgtcagtc gctgcacgtc aattcctatg acgaagcgct
     3121 ggcgctgccg agtgaacagt cggcgctctt ggcactgcga acccagcagg tgctcgcgta
     3181 tgaatcgggg gtcgcggaca ttgtcgaccc gttggggggc agctacgccg tcgagtccct
     3241 gaccgaccaa atcgaagcgg cggcgcaaag ctacatccag cgcatcgacg agctgggcgg
     3301 catggtggcg gcgattgagc agcgctatcc ccagcgtgaa atcgagcgac gcgcctacga
     3361 ataccagcgc caagtcgaaa gcaagcagcg catcgtcgtc ggtgtaaacg actttgtgat
     3421 cagcgaaccg ccgcccaagg ggctgcatca cctggacccg caggccgaaa tcgatcgcgt
     3481 caccgagatc cgtagctttc ggaccgcgcg ggatgagcaa aacacccggc aggcgctgcg
     3541 agcgctcgac caaaaagcga gccgtccgtc ggcgtccgac aacaacctga cggcggcgat
     3601 cctcgatgcg gtcaaggccc aggccaccgt tggggaaatc gccgatacgc tgcgcatcgt
     3661 gtttggcgaa taccaaggct aggcctttgg accattcggc ctgggcgtcg tttctcgact
     3721 tgaaatccgg ccagatcctg ggttacacgc cccgcacatc acggaggatt ttcatgtcag
     3781 atcgtcgttt cagccaaggt acgctcagtt ttcgtcgttt ttgggcaggt tcactgctcg
     3841 cgcttgcgct ttctacgaca ggctgcttcg gggctcacta tcgacccagc tacagcatgc
     3901 aaaatgtgca ggccaagccc gcgagcctga tggtcgcgca gaagattcag cggccccttt
     3961 tcttggttgt cgattcggcc aaagtgcccg atagctggga tctcaaagag tcgtcatcgc
     4021 tgaatcccaa agacggcgag cagaccaagc tcatcgattt ccagcagttt gtccgtcgcg
     4081 atctgaaaga agcgctgggc aattactttg ccaaagtcga agtggtcaaa gccggaacgc
     4141 cgctgcccgc agaaccgcac gtcgtcgctg atgtgaaagt cgatcgggtg cagctccaca
     4201 gcacgcccgt cggacggctg gtctacacga tcatcgaaat gacctggggt gtgggcctgc
     4261 gtccgagcga acaagccgaa tatgcatttg cgtttgccgg cgaaggcaag agctcggaat
     4321 cgtatccgac attcgaggtt ggcgtcgccc agctggtgga atcggcgatc ctgggattta
     4381 acaagagcct ggtcgaaaag ggcggtctcg aagcgctgca aaaagcgagt gcgccggcgg
     4441 ctccccaggc tgctgcggcg gccaatccag cgggcggtgc ggcggcgcag gccggtggtg
     4501 aaccgctcga cctggcggca ccgggcaaac cggccagcaa ggccggcaaa aagcgcagca
     4561 agtagcgttt atttggcggg gctcagctct ttgccacgca ggacaaagtc gcggaccgat
     4621 ccactggtgg ccgaaaagct gatcggcttt cgcaggatga cttcggcttc gtcgccggcg
     4681 ttttggatca cggtcagctc gccttggtat tcgctggcgg cgtcgccccc ggacttgtag
     4741 acgcgcagct tccattcgcc cgcgtcgatg cggtcgaaac gctgggcttc gatgccgacg
     4801 ctgccgcctt ggagcgcggc cctggtcagt tcgctgctgc gtcccggtgg ctgaagctga
     4861 agctgaagtt gggcgtcggg atgcgaccac gtcaccgcga tgagcagctt gcctgcatag
     4921 ccagccagac cgtcgctgcg gccacgtact ttgagctgcc cgagcaggtt ttgatcgcct
     4981 ttcttttggg catcatcgac aagccgcgaa agccgcacgg cgtacagcgc gcgcgcccac
     5041 gccggggctc ctttttgcgt tttttgggcg ccgaccgctt cggccactcg gctcgtcaag
     5101 cgaagcgctt cgtcgctgcg gccaagtccc gccgccgcgt cagccaacag cagcatcacc
     5161 gcttcgtctt gcggcaaaag ccagctaagc ccctggtatt cgcgatacgc cgcgtcgtaa
     5221 aagccgtgcg cccgatacaa atcaccgagc cttcgacgac cccatggatc aaacggcgca
     5281 aactcgacga tttccgacag tgcgcgggcg ccttcttggc cttcgccgcg acggatcaga
     5341 tactcaccga cagcctggcg cactgatgcg tcggcacccg gatcgctgcg ggtggtttcg
     5401 atgagtcggt tggcttcgga aaacagcgct tgcttgcgct cggcgctgac gcctttttca
     5461 ccgctcgctt cttcgagcag cgccaacagc ttgagcttga gcgacaacga gtcgggggcc
     5521 agcaaaagga agcgtcgcac cacgacgagc tgatcgtcga cagacttggc tttcttcagc
     5581 tcctgcgcaa gcagcgtttg atcgacacca ccatcgagag ccaggccgct ttgggcggcg
     5641 cgaatgtcgg cttcggtccg caccgcgcgc aggatctctc gtcgcagatg tgactggatt
     5701 tcgtgcgcgt tttcgctgtc gtcaaaccga tggtacaggt cgacttgctg cgcggtggca
     5761 ccgacgtgaa ctcgcaacat gtcgatgatt gcccgccggt ctagccaggt cttaagctca
     5821 cagcgcgccg ccgcttcgcg atagaccgtc atcgcgccct cgacgccggg atggctggct
     5881 aggcgctcgc gccacagcga ccgtcgcacc tgcaaataag cccgcgaagc ggcactacag
     5941 cgctgcggtc catcgtcgcg atcgggctgg ttgagctgga gcgcatagtc gatgtcgtgc
     6001 agttttccat cgggtgacgc cggcagcttg accgccagca gtgcccgctc gatgcaggca
     6061 tcaatttctt tcgatcgcag tgtcgacgaa ctgacgcgga catctttggg aatcccgtcg
     6121 aggccgagct tgacttttag ctcgacgcga cccgacaggc ctggattgcc ggcggcccgc
     6181 cgctcgtagc acagacgggc cgcgctgctt tggccgttga gcgcccgaac gtacatttcg
     6241 tttacgctga tggtgcggac gtttgccgtc gagacacccg attccagcac aatcgacgag
     6301 tcctgcggtg ctgcgccgcg caaggccggt ggaacaaact gcccggcttg caggtcgcca
     6361 accagtggtc gataggtttc accgcccgat ccctcgacga caatggcgct ccacggcgtc
     6421 accacttgga agcgcttgcc gatgtcgacg atggcttctt tgcccgacgc gcgttccagc
     6481 agcccttgca tccgcaaaag cgcccagcga cgagcgatgt cgccgccatc atccatcgtc
     6541 cgcacggtca gatcgcgctg ttcgacaaac ggttgtccgt cgcgaacgcc gcgtacttgc
     6601 agcttgccgg gcagtccgcg gtcttttggc agcctggcca ccacggtcag ggcatcgccg
     6661 ctttctagcg tgaccggctc gtcgggatag accacatcgg cgccttcgcc cagatcgacg
     6721 gtgacattgg ttaggctcgg ctgggcaatt cgttccagca ggtagagcgc tgcccgcgac
     6781 gcatcgggtg catcgtcgat ccgcaccacg cccgagcgaa ttcccgagtg acaaagcgat
     6841 tcgagaagcg gcaaattggc gtccgttccg acggcagcag caaaaaaacg cggcagatca
     6901 cccagccgct ggagctgttc gcgcagctct cgcggggtca gggctcccac cgtcgggcgt
     6961 ccgtcgccga tgtagatgac tgcgccttgg ccacgcggca gaagctcgac ggcgcgctgg
     7021 aacgacaact gcaaattggt ggcaccgccc ggcgtctgtt tcgccaattg ggcatcgagc
     7081 tgttcaatcc gttccttggt tgcgggtgcc atcgcttcgc tgctcagcgt tgctgcgcca
     7141 agtgatgcgg ccacgacggc gacgcgatct tcgggggtga gccgttttaa gacagcctgg
     7201 gcgacgctgc gaccgaggct gagcttggct tcgtccgttc ccgccgacac atcgacgagc
     7261 agcacgacat ccaggccttt gggggctttg cccggcgctg gcgtcggctg aatcagcaga
     7321 tagcgctggc catcgagttc ttcgccgcgg aagccaaaca gccgttcgct tatccggtct
     7381 ttgccgtcga tcaggtcgag cacaaagtct gccgtcgggc gaaaatcact gcgtcgcagc
     7441 gtcacctgcg aattttccag ctgtgcgccc attcctgctt gcagcgccgc cgcgccggac
     7501 ttggacacgt cgacggtcag cgaaaactcg ccgaggttcg acagcgtgcc tgcctgcatc
     7561 tgcgcgctct gtcccatcgg atagacgtag gttcgcttca ccgcatcggg gtgcgggatg
     7621 tgcggcagcc actcgcgata gagcaaaacg acccgacgga ccttacctgg cgcaatgttg
     7681 cgaaccagac cacgatagcg gccagcgcca gcccactcca ggttggccgc aaagctggat
     7741 acgccaccgt atttggggtc catcgacagc accacgccac gacgcggcgc acgatcgtct
     7801 tcagcgacgg aaaaatgttc caaaatcgcc gactcgggca gccgcacggt atacaggcct
     7861 tccaggtatt ccgagcgcgg attgaaaaag gtctgctcga cgcgggttgt cgccaagtcg
     7921 ccatcaatcg tgacggtgac ttcatgcgaa cggacgataa gcggtgtgcg cgcggtgcca
     7981 atgcccgatg tcggacgcgc ggtaagctga ccgacgccgg ctggctccag cgcacgcagc
     8041 ggtcccggtt cggcgagtcc cagcgtaaag tcgtcccaca gcgcctgcgc cgtctgtttg
     8101 gcttcctcgc cactgaccag cagcgtcaga cccgattcca gtcgaccggc tttgtcgccg
     8161 cgcttccacg ccgcttggcc gctggcgcag tacaccttgg caccgccgtc tgtgatggtc
     8221 acggcaagac cggcccgctg cgccgacagc tcgacgcctt gggcaaccac cggaacatcg
     8281 ttgccgacgc gggcatcaat ccaggcgcga ccgcttttga cttcgatgcc tttgtcgcga
     8341 atcgtcacct ggctgctctg gtcgagcagc acccacgcgc cggcatcgag atagagcgac
     8401 gcgcgcacgc ccgcaggcgt cgtcacctcg ctgccgacct cgagccgcac gttgcgcaca
     8461 atcggcagcg tcgtgttgcc gcgcttgatg tgggcaccat cgcggcttgc ttcgaccgat
     8521 gccaccgtcg gctgcggcgg cggaattttg gtacacgccg aaagaccaag tagcgaaagc
     8581 agggccaggc caaaaagccg ccgaaggcga gccgcaccga ggcttctgcg tatctgaggt
     8641 tgagcttgta gccggttcat tgcgacccct tactgtggca aaagccgtcc gtcgcggagc
     8701 agtagccgcc gttcggtcag ctctcctgcc agcttgaact tgctggagag cagcttttcg
     8761 tctttttgac cctcacttag caccaccgtc agcaccacgt cgtagccaat cgcgcgcttt
     8821 tggtcttggc ggcgcacttc gacgacgtac tcacccttgg atgaagcgtc tttgtggctg
     8881 tgccaaccga gcaggccaaa ctgtggcgcc agatcgatcg ccggcccgag cgattcaccc
     8941 ggcgcaccga cgcggagatc cagatccgct tcgggatggg cccaggtcaa aaacacccgc
     9001 accggacgat acagcccctc gatgccgttc tgacgggcgc gcaggtgcaa aagcgcaagc
     9061 tcctcggcgg tgtctttgcc cggctgggtg cgtgcttgct cgcgcagctc ggccagacgc
     9121 aggctcgacc acagaagcgc aatcttggcc agcccgcttt cgctgcccgg ctcggcatca
     9181 ccgacgacac gtttttccag accgagcgct tcttcgacct tgcccgcacc cgctgccgcc
     9241 gacgccagca agatcagcac cgagttgtcc cccggctgaa gctgaagcag cgtctcgtac
     9301 tgacggtagg cttcatcaaa ccagccgtgg gcgcggtaca aatctcccag ccgtcgacgc
     9361 gccaggggat catcgggaga aaactcgacg atttcggaaa acgctcggcg cgcaccgatt
     9421 tcgtcgccca gacggacaaa cagctcgccg accgacgttc tggcttgggc gtcggcatag
     9481 ggatcttggc gaatctcctc ggcagttcga cgggccgaat ccgcttcgcc gagcgactct
     9541 tcggtttcca tccgcatgat ccgcagcttc agatcgccgg gccacttggc cagcagctct
     9601 tccagtcgcg tcagcctctc tggcatcgac ttggctttgg cgaccacttc ttgcgccagc
     9661 atcgcgcgtt cttcgtcgcc aagtcccaaa ccgtcgtaga tcacccgcag atcgcgcgga
     9721 ctgcgcactt cgcccaaaat cgcatgccgc aggtattctt gatcgtcggg ctcgtcggac
     9781 aggttgttgt aaagatcgac catgccgctt gggccgccga cgttgcgcag catcagccta
     9841 agcagctcgc ggcgctccga ccagcgacgg acttcgcagc cgcttaccgc gtcttgcatc
     9901 acttccatcg ctttgcgcac gccgcccgac agccgttctt tccaaagctg ctttctttca
     9961 tccagcggct gcatcgacgc tgccgagcag cctttgggcc ggtgggtcaa aattccgacg
    10021 acaaccgtca cctccggggt cgaaatcgcg accgccgggg ctgattccat gtccggtgca
    10081 gacggataat tcacattgct cttgagaatg aacggatagg acacgacgac gatgccgccg
    10141 cgtggccgtg gaaattccca gcgccgaatg gcgccagcga tacacgcatc gacgaccgga
    10201 ttgcccaccg tcgaagactg gatggtactc gccagcacac tgccttctgc gccaacggtg
    10261 aactggacga cgatgcgacc gtttccggcg tgaccgcggg atagctcacg ctcttggcag
    10321 aacttcactt cattcatgtg gcgacggatg acgcggcgga tgacttcttt gtcgagcgat
    10381 ccgcggactt ccgcattgcc ggcgatgaca tcggcgccca ggtcgctgcg gcggtttttg
    10441 gcgccgtatc cagatcctcc gccgtggccg atggttccga ggctgccgag cccgatggtg
    10501 ccttcgccgg tgccaccacc gcctttgcca ctgccgacca gaccgaatgc atctccatcg
    10561 agcgcgtcgg ccagggaatc ggctttgtcg cgacttgcgc ttcgcttggc ttttctgtct
    10621 gcgccaccgt agtcgccgcc ttccgacacc gcttccttca tggctcgcga tgctttgggc
    10681 gcgggggctg ccgcggaggc aggctcggcg ggcggtggtg ccggcggtgc ttcgtcatcg
    10741 gctggggcgc tcttggcggc ttcgccttga tcaacatctt tgttgggagc ggccttacgt
    10801 tttgcggctc cagcgcggcc tcccatggcg ccttcttcgt tgcgggcgct gctcgatgga
    10861 tctgccgaga ccacagtggg cgatgaggga gccataaacg ccggggcggc ttccgacgcg
    10921 ctctttgcaa acgacatcgg cgaacggctg ctgcagcccg cggcgcgcac ttccggaatc
    10981 gcttgttcga gcgcagagac ttcctcttca ctcggcacgt agtacgacgt atagggcgta
    11041 atcaggtttt ggcgcacacc cagttcggcg acttcttcga cgctggcccc actttctagc
    11101 agctgtgaaa gccgcgccgc cgaccagcgc aggcgcagat ccgacgtgtt gtctatcggg
    11161 tggccttcga gtcgataggt gcgcttaaac ggctgtccaa gctgcacgcc ttcgacggta
    11221 attgtggacg gcaaacgctt tttcagccgc gccaaaatca gcagcggctc gccggcccgc
    11281 accgcgacgg tttcccgtgg atagacccga tcgagcgagc tgccgacctc gaactttagc
    11341 ttgctcaccg ttggacgact gaggtgtgag accactttta gcgcggtttc ggcggcgctt
    11401 gcgcgatccc cgacgcggta ggcttgtccg gcgctgactt ccgaaagacc tgacaaaaga
    11461 tccaggtttg cgtcgtcacc aattccgacg gaataaagac gcagcggctg cggcaggctt
    11521 gcgagtcgtt cgcgcaggtt tttcagcgac agctcaccga cggtcggttt gccgtcacca
    11581 acgtacagca ccgcgccgat ccggctgggg tcgagcatcg ccgccgcttc actgagcgtc
    11641 cgtcccaggt cggtcgcgcc gcccgtcttt gccctgccga gctgttccaa aagctggttg
    11701 gcgacctcgc cgctcatcgt cgaccacgtc ggtgcggcgg ctttttcttt ctttccggtc
    11761 ttgccgccca gcaccgcatg cgcgtccaga tcggccccca acagcaccac ccgatcgtcg
    11821 gggcccagcc ggcgcagcac cgattcaatc agcgtccgtg ccatcgccag gtgcgttgca
    11881 tcggtggccg ccgacacatc gacgagcagc gccagatcca tcggcgcacg ctctgttgcc
    11941 gtcggcagtg gcgctggcag ggtccgaatc agcacgtatt catcttcggg cttgcccgaa
    12001 tgtcccatgg gccgcggcgg atcttggtgg gggacgtgat agacccgcgc gacatcatcg
    12061 agccgcttgt cagagaccag ctcgacggtc agatcggcac ttgggacaaa gtcggttcgc
    12121 tgcagcacca cgcgcttgtg gtcatccgac agcaccgccc cgagccccga gcgcacttcc
    12181 ttggtcccgg cgtggctgac atccacttcg atgtcgagct cttgaatgat cggcgcgcct
    12241 gcccccgccg cggcacccat cggaaagcga taggtccgca agctgttgcc aagacctgcg
    12301 agcggcagcc actgcgaata ggtgtaaagc acgcgccgcg tcgagcccgg tggcagcgga
    12361 tacagccgcg ctttgtactg ccccggtgcc tcccattcga gcagcgccgg atcgtgggtg
    12421 gaaccctcat agactttcga ttcgtagtcg cggcgcgccg tctcgcgttc tttgacgtaa
    12481 ccgtcgacga aacgcccacg ccggtcgatg gcaaagcgcg acaaaatggc gtcttctggc
    12541 agccgcaccc ggaaaatgcc ttcgagcgtc gactcatcgg ggttaaaaaa cgtctcctcg
    12601 acggtcgtaa tcgccagatc gtcttcgatg cgaacccgga cttccagtcg attgagcgac
    12661 agcggaaacc gcgcttcacc gaggctcccc ggcttgcgtg cgcccacttc gccaagtccc
    12721 ggtgcaccgc gaccgacgcg cggatcgggc catgccagac cacccgtcca gtcatcccac
    12781 agcgcaatcg gtgtcagttt tgcggcattt gcttcgacgc tgagctgctc accggaatga
    12841 acgacgccgc gcccactgcc gacgtaagcg acctcgccac ggatcaccga cagacgggcg
    12901 ggcccggtgg ttttgctgac ctcaaagccc gcggcagatg cgagcaggct cacatcaccg
    12961 acgcccactt cgagccggcc cccacttttg gcgtcgacaa agatgcggcc tcggctgatt
    13021 ttgacgccgg tgtcggtgac gatgacttca ctgccaccgt cgacaagcag cctcgcaccg
    13081 acatcgtggt ggatgattgc ttgggctcgc tcggccacgg tgatggtctg atctgccgaa
    13141 gcccgttctt cgccgctgat tgcgtgacgc gacgacgaaa ccgcaagctc gacgggagtt
    13201 cggaccactt cgacgctggc gaccgagctg accgcagaca gcttgggccc gcacgccgat
    13261 agaccaagcg aaagcgcgga acacagcagc gcaaaaagcc gcgtgtgctt acgaagctgg
    13321 gccagccagg gatgacccgg aaggcgatgg gcataccgca taggacctgc ctcccccttt
    13381 gggtttttcg aggggaagcc gatatcttat cccgcacggg tcgggtcagc caacgtgatt
    13441 tgctgtgcgg cgggtcgcag caggatgcaa tgcgcgcttc acgttttttg atgcttgcgt
    13501 tcatactcgc aaggctttcg ggaggaactt tgatgacagc gaatgcaggt gcagcggcga
    13561 gcggacgaca cttgatggac tgctttgccc aaaccgtcga ctatgcggcg cgcggcgcag
    13621 tgtattttgg caagcggatt gccgtcgtgg acgtcgagcg gggggatgcc gggcggtttg
    13681 actacctatc gctttcacag cgcgcgaccc ggctggcccg gtttcttgtc gaaaaagggg
    13741 tccaaaaagg cgatcgcgtc ggactccttg cgcaaaacgg catcgagtac atcgatctgc
    13801 tgctggcctg cgccaagctg ggtgccattt tttcgcccta caactaccgg atgcacccgc
    13861 gtgagctgct ggaagtcgtc gaaaagaccc gtccgcgggt ctttgtctac ggtccggaat
    13921 acaaggcaca tgccgagctg ctcatgggtg aacaggcgac gatttcgtct gtgaacacat
    13981 tccttttggc tggtctaaat ccacagcttg gtcaaggtac gcagtcgttt gaacaggtgg
    14041 tgacggaagc tccgctcgac ggtgcgccac tgttttcacc cggagatggc cccggtgggg
    14101 aagacatact ctgtctgctt ggaaccggcg ggacgaccgg acttcccaaa gcggcgatga
    14161 tttcacatcg aatgtgcgcc cacaacgcgc tgacgacgat gattcacgag cttcagcccg
    14221 gcgatgtcac ggtgattcac accccgatgt ttcataccgg cggactcctt gtctatgcgc
    14281 tgccgctgct tttttcgggc ggaaaagttg tgatcatgcg caagtgggat tccgaacagc
    14341 ttctgtcatt gattccgcgc gaacaggtga cgatgttttt ctgtgtgccg acgcagtacc
    14401 aaatgctgct cgattcccca agctttgcca agacctcgtt tgcgtcggtg cgctttgtca
    14461 caagtggggg ggctccgctg ccgattccgc tgattgcggc attccgcagc gtgcatgaca
    14521 ttccgttcaa acaggggttc ggaatgacgg aatttggtcc cggttgtttt agcatggggg
    14581 ccgagtacgc acagagcaaa gccggatcga taggacgtcc caacgccttt gtcggtgcgc
    14641 gaattgtcga cgaaagcggg gtcgatgtcg ctgtgtccga agccggcgag ctgctgctca
    14701 aaggaccgtc gatgttttcc ggatattttg aagacgacag cgcgacccgt gctgcgatcg
    14761 atgcgcaagg atggttccat accggagaca tcgcgcgcag tgacagcgac ggatttttct
    14821 acatcgtcga tcgcaaaaaa gacatgttca tctcgggcgg cgaaaacgtc tatccggtcg
    14881 aaattgaacg cgcgctgtat gaacatcctg cggtcaaaca gtgcgcagtg attggcattc
    14941 ctgacgaaaa atggggccag gttgggcgag cgtatgtggt tcttcatgcc gaaccggcag
    15001 gcacgccgct tccttcggaa gagtcactgc tcgactttct gcgcagcaag ctcgccagat
    15061 acaaagttcc caaaggggtc cgtttcctgt cggagctgcc actgtccccc gccggaaaga
    15121 tcctaaaaag agcactccta gagaagtaat atcatcgctg cgcggtgtct ttcccaaagc
    15181 cgattcatcg cgcgcagttc cctttctttg gcagatggaa atcaggtgtc cggacggctc
    15241 tgttttgcaa tcggtggcag tccggcaagc gctgtatgca gtcgttctgc cgtcggtcgc
    15301 atcagtggat cgggatgcag acatcgttcg agcagcccaa gcagagagtc cggcagcgcc
    15361 agcccaagcg accgaaacgg aaccgcaact ttcagatcgc tcggtcgctt ggcccacacc
    15421 actggtgatg aaaacggcat ctctttggcc agcagctcat acgcaatcac ccccagtgca
    15481 aacagatccg ccgcaggccg cgcatactta gacccactcg ccagctctgg agccatgtac
    15541 atcggggtcc cgtgcgatgt gtaattaagc gggggtgcca aggcgtaggt acaagccgca
    15601 gaaagaccca cgcgttgatt ccgtcgttaa gtttcaatac gatgggaatg tgccggagtc
    15661 tgcacctagt gtcgtcggat acctgcgggt atcgaccaca gaacagaagg atgaaggagt
    15721 gagtctggac gtgcagcgcg aacgcattgt cgcatactgc aaaatgttcc agcacaacct
    15781 gattgcgatc tatgcggacg accacacggg acggaatctg gagcggcccg gattccaagc
    15841 ggcgcttgcg cggatggtcg agacggactc gatcttgatg tctgtcagca tggatcgcat
    15901 ctcgcgaaat gtaggggact ggacgtacct gctcgatgcg tactttggga aagggaagtc
    15961 ctacaagctg ctcgcgttcg actgcgcagg aatggatcca cgtacagcga caggtcggat
    16021 gctcctcatg atgcgggcgg tgatggcgca gggggagatc gactccacca gcgagcgcac
    16081 gcaagttgcg atggatcacc tgaa
//
LOCUS       DAORGN010000115        16058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1489_length_16058_cov_5.899331, whole genome shotgun sequence.
ACCESSION   DAORGN010000115 DAORGN010000000
VERSION     DAORGN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 16058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16058
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_16058_cov_5.899331"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKL17_13170"
     CDS             <1..336
                     /locus_tag="PKL17_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45733.1"
                     /translation="KAVDQTRRTEYLQRQVSRLRELVDRLSKGGREERDAMTAISESL
                     QRMVRTLEDLANSQRSNASEGERLRNLMEVVRRGTTNQREQSAALDRCLGELGTQASE
                     LRGQLSKRN"
     gene            complement(320..1039)
                     /locus_tag="PKL17_13175"
     CDS             complement(320..1039)
                     /locus_tag="PKL17_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HNK45734.1"
                     /translation="MNETVTTRFDLLRDVEFVRALDSAVQRHAMLKHPFYQAWSEGAL
                     SLDDLREYSKQYFSQVKAFPRYVSAVHSNCDDIALRQELLQNLIEEEQGEGNHPELWL
                     RFAESLGVDRRDVESAEPTRATQASVEIFRDLTKNGSYLQGLAALYAYESQIPDVAKT
                     KREGLGEFYGIRDPRAVSFFSVHEEADVVHRQVEVDALLSHAKTVEQRVEVLSAAEQA
                     SHALWQFLDGMQSLRGASCAC"
     gene            complement(1156..2016)
                     /locus_tag="PKL17_13180"
     CDS             complement(1156..2016)
                     /locus_tag="PKL17_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK45735.1"
                     /translation="MGSFLLPVAKAQLHVEWVFPQNEQPKNQPPVLILHGGPGADSRY
                     LRPQLDRVADLGAGRPIYFYDQRGADRSPQDEGEDPPSVAIHVEDVDAVRRYIGVDKV
                     RLLGYSWGALLSMLYATKYPQHVEKLILLSPAPATAEVRQTYQRRMTEAMQRPSVIAL
                     RDELARLRETATPEQQRRHRFALAVSSYFVNPRRALELTPFLVKQRLEEAIWKSLGPQ
                     FDLRPKLHELAHIPTLVIHGTDDVIPIESAAETARILSAELVALADCGHVPYIEAPEP
                     LFAALTRFLA"
     gene            complement(2019..2804)
                     /locus_tag="PKL17_13185"
     CDS             complement(2019..2804)
                     /locus_tag="PKL17_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45736.1"
                     /translation="MSLLDQPFGFSGERKAFAILCLAFYMALYGLLSLLFYLAPPDQP
                     EVRAWVPAFLALSLTYGLSFFALAADWFWARWFAVGLGYSGLTVAMWGMVTARTIDPV
                     LLFYGMTHGIVALFLQGQKLADHFEGQKSWRELLRVTDDQGVEKVRHTVTRAAASLPT
                     LILIALAPKEGQAALLVLVGVGLFAVLRNRTAGVMLFLGTALALPLSLLHTHATEAVT
                     LPSRFGVLFTPVHLHQLAMLSSALLLAAALPYLRPMIQFVRGR"
     gene            complement(2916..4940)
                     /gene="mutL"
                     /locus_tag="PKL17_13190"
     CDS             complement(2916..4940)
                     /gene="mutL"
                     /locus_tag="PKL17_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNK45737.1"
                     /translation="MPRIALLPEELTNQIAAGEVVERPASVVKELVENSLDAGAQRID
                     VDIEEGGLKLVRIADDGCGMNPEDAELSLLRHATSKLRSADDLFRLTTMGFRGEALAS
                     VASVSRLQLTTRTKDAVAAYRITTESGQKQSPSREVGGPVGTQIEIRDLFFNVPARLK
                     FMKTEATETGHISETLIRLSLAYPGVHFRLRALGKDKERVLLDLPPHRSSLERSKAAL
                     ASRGKGQGAPVLYQGESQAPGISVEVHLGEPDDATTTARNVFLLVNRRFVRDRSLLQA
                     VTTGYGELLEKGRYPLCVLHLQLDLELVDVNVHPQKLEVRIANADAVFSAVQRAIREV
                     CVTAPWLLGRSRPAQSERTYVLGTGLSAAEPRPSYGTTTVAPPMSLPSQPPPISVPQS
                     PSQAPFLAQAATVAKLQPVERVATHSVPFLREPPRPSGLSEHRSRLQQALKLFAPIPD
                     SRDAMQADDKPPSPTAEPHDEQRIQLAHLPYLGQLFATYLLCEYENELLIVDQHAAHE
                     RVLFERLRRAHGQTQLASQRQLFPIHLELDDKRVALLTEFEAALHHLGFEVKPFAGKT
                     MALLSCPDLGQYGRGATVHSEPERLLRQVLDELEEHGRSDALQKRDDLLLATMACHAA
                     TRAGDVLDEAKARALLLALDDVSLSPYCPHGRPVLWRLSKMEVERRFHRT"
     gene            complement(5058..5249)
                     /gene="rpoZ"
                     /locus_tag="PKL17_13195"
                     /pseudo
     CDS             complement(5058..5249)
                     /gene="rpoZ"
                     /locus_tag="PKL17_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            5461..7305
                     /locus_tag="PKL17_13200"
     CDS             5461..7305
                     /locus_tag="PKL17_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNK45738.1"
                     /translation="MGLMRLWVRGGFVLASLLGVSMQLASAVPPAAQAAPSPPPPIVA
                     GQFAAARGTDFVIGDVPFRFVGANINPIHGDSDRRATQALFEAMGKDGLNVARVWALG
                     EGMPDAGEWEQKYALFRAGPDRFLEDAFLQLDRILMEARRAHVRVILTLSNNWADYGG
                     VPMYLRWLGLPPDGMAKEAFYSDERAHALFRAHVGRLLARTNSLTGIRYSEDPTIFAW
                     ELMNESTVETPAGRKARLAWVREMAQFIKTFDPHHMVAAGLWGYAMRSERADFVAVHK
                     LANIDYVDSHLYLQNSQGNVSLPRLYDFLDDRAQLARHVIKKPLLIGEFGFRTDKGPT
                     YLGLSRARWFSELLLRHFRNRGGGALVWIYEPYSGKPRDFGIYIDKSTTDDVRQALRT
                     VAAQIRSPLADNPRLAPSLGDAPLYAVDQVLHGLLRPRLLWQKAHGGELSLRLAPSEF
                     RVAHFERLGVWDGQPAAHFYGAESGDLVFGFSAPPAMGQKALAEVEVRARLSSEWPGA
                     QAPADGGSLVQVELDGMLIGQSTVIPDDGHGAWYSFRTSDPRILGKLSTGEHTLRLHV
                     PPGPLAHGLCLYGDARDPKLVKTDYGPLQILLHSAIPATGNSQAQTVK"
     gene            complement(7312..7788)
                     /locus_tag="PKL17_13205"
     CDS             complement(7312..7788)
                     /locus_tag="PKL17_13205"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK45739.1"
                     /translation="MRLLKTVESFLREDGWDFAQLENRSVLKTGYQGEHGSWTCYAQT
                     REEDQQFLFYAVCPIRAPEKKRAAVAEYITRANYGMVIGNFEMDYSDGEVRFKTSIDV
                     EDAELSESMVRSATYWAVVMMDRYLPGLLKVIGGSTPPAEAIDEIEAEDHPDPSKS"
     gene            complement(7832..8578)
                     /locus_tag="PKL17_13210"
     CDS             complement(7832..8578)
                     /locus_tag="PKL17_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNK45740.1"
                     /translation="MILLFPKEHGAYGQLFLPLLTALFLHGMPRLATLLLFLSASFLF
                     WSHEPLLVLMGHRGTRAQREQGRMASRLFPLLLLGTLLGILGALFVRPQIGWTFLLPL
                     AMGLCVVPFMLLRHEKSTLAELLVALSLSSWVVPLQVSKSVPLSLSLHLWASLGFLFC
                     VAVLSVRGLMNKIALGWSVGLALAGIALWAGLAQLWTLPRENIWPQIPTVALAIGLAA
                     KKPGPRALRQVGWAIVAATVVQIGLLQLSR"
     gene            complement(8590..11130)
                     /locus_tag="PKL17_13215"
     CDS             complement(8590..11130)
                     /locus_tag="PKL17_13215"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017295861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNK45741.1"
                     /translation="MSSDGATTTPCPPIIQVEDDRTSYTEEGFSRGLLDHLHFTLGKD
                     DTHATQHDRYLALAHTVRDRVMAKWMRTKDAYRKQDPKRVYYLSAEFLLGRALQNNLL
                     SMGLYDKAQSVTGRLGLHLGDLLDQERDAGLGNGGLGRLAACYLDSMATLGLPAYGYG
                     IRYEFGIFDQVIRGGAQIERPEEWLRYGSPWMVARPEFTQPVHFYGHTEVHGNGHPQV
                     RWVDTTSVLGVPFDMPIVGYKNDTVNTLRLWSARASEEFDLTVFNAGDYERAVFDKNN
                     SESISKVLYPNDLLVVGKELRLKQQYFFVACAIADLLRRHLDHAKNRHDPQAIRERLL
                     MLPEKVAIQLNDTHPSIAIVELLRLLIDKYELPWDTAWQLTESVFAYTNHTLLPEALE
                     VWSVELLGKLLPRHLQIIYEVNHRFLRQVWTNNPSDQERLQRMSLVQEQPFKGVRMAY
                     LATLGSHKVNGVAELHSRLLREHLLPDFAGMFPDRFINVTNGVTPRRWLLGANPELAE
                     AITRRIGDSWPTELEQLERLVPFAKDPDFQAEVQAIKLRNKQRLCEYIARHNHVTVDP
                     HALFDVQVKRLHEYKRQLLNVLHIISLYQRLKQNPSLDIVPRCFLFGAKAAPGYTRAK
                     LIIRLINAVADVVNHDPHVCGKLKVLFLANYRVSLAEKIIPAADLSEQISTAGFEASG
                     TGNMKLSMNGALTIGTLDGANVEIRSFVGPENFFLFGLTADEVMAKKASYNPWDCYRR
                     SPDLHSAIDLIRSGFFSYDEPLRYQPLLDALLPPEEKPGRGDPFLVLADFDAYAACQQ
                     AVDQAFRDPQKWSEMAIHNIARTGYFSSDRSVREYATKIWNARPVKVT"
     gene            complement(11202..12878)
                     /locus_tag="PKL17_13220"
     CDS             complement(11202..12878)
                     /locus_tag="PKL17_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK45742.1"
                     /translation="MNTGSVSRRVVIVGGVAGGASAAARLRRLDEFADITLIERGPDV
                     SFANCGLPYHIGGEIASRDALSVQTPASLHAMLNLRVLANTEATDLDRTARTVTVVDR
                     MSGQQSTLPYDKLILAPGAAPLRPPLPGIDHPQILTLRNLVDMDRIKTALHNVHSVLV
                     IGAGFIGLEMAEQLAHLGKHVHLVELQPQVLPLLDAEMTRPIEHELRHHRIDLRLSTA
                     ITSFEAGPGHKVTAITKTGERLTVGAVVLSIGVRPESTLAQKAGLLLTPRGHIAVNSF
                     MQTSDPDVYAVGDVCATPDPLVGGVSNVPLGGPANRQGRVCADHIVRGDAAAPYPGSL
                     GTAIVRVFHLAAGITGLSEKRLQELGRPYESTLVSASSHASYFPGAQTVTLKLLWEKT
                     TGRVLGAQAYGADGVDKRLDVLATALRGELTIDDLVHLELAYAPPFGSAKDVINIAGF
                     AATNRRDGLVNPVQSLPALGTAQIVDVRSAAVSAAHPVAGAVNIPLPALRHKLAMLSP
                     DVPVVTVCAHGKTSYFAARVLAQHRYQVSSLAGGVYAHPDILAKTEETQS"
     gene            12987..13763
                     /locus_tag="PKL17_13225"
     CDS             12987..13763
                     /locus_tag="PKL17_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45743.1"
                     /translation="MDLQSISRAKAGDKAALGQLLEEIASPVYRFSRRMCGSSADAED
                     VTQETLLSAAQNLSQFAGQASFQSWVFAIARSACSHRYRGKKNQPHQTEDSLLALAAA
                     TPDPETAVLSHEHAAVVKSGLGMLPPDQREVLWLRDVEGLSGQETADALGLSIEAVKS
                     RLHRARQMLRQLVSTKLQLALEQPLSSCPDVVEMMSRKLEGDLAMADCAQIEKHVANC
                     PRCHGRCDEMRQVFVLCRQAATESAPSEVRIKLRRLLNAL"
     gene            complement(13862..14692)
                     /locus_tag="PKL17_13230"
     CDS             complement(13862..14692)
                     /locus_tag="PKL17_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNK45744.1"
                     /translation="MPQKPIEVNLDLNQMLGLNRPKQGGGPDPKALERAVTLVNQERG
                     KLGLHPLSGVPQLFQVAQAHSEDMNKRGYMGEATPEGDPVSGQVRRAGYEGRCEVLVA
                     DGAEQPDGVIREWLAHAQYQRHLMSETYQHLGVGVSDGLWTFVLGMPPSLQMTDVKEL
                     RFAVLKHVNEERRRANLPLLELSDTLSSAAQEHSADMAQKDYFGTASPSGDSIASRVA
                     KAGFKGRSVGCLTKGPQSPDEAVAALLKTSRGNLLHPEVCFLGAAVTATRWTVILGTR
                     "
     gene            14781..15869
                     /locus_tag="PKL17_13235"
     CDS             14781..15869
                     /locus_tag="PKL17_13235"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNK45745.1"
                     /translation="MSKVKTIAISTGGGDCPGLNAVIRAAAKSAILQHGWRVLGIEEG
                     YNGLIWPDRIKELTLWNIRGILPRGGTILGTTNRSNPFAIKIVENGTEVTRDLSDSVI
                     QNARSLGIDALINIGGDGTQTIGLKLHKKGLPVVGVPKTIDNDLSATETTFGFDTAVH
                     AATDAMDRLHTTAESHKRIMLVEVMGRDAGWIALHAGIAGGSNVILLPEIPFTIERVC
                     EHIRKREFSGRRATIIMVAEGIHVPKDLPADLIEEWNRTPRRGAVSHLIGEAVSRHAG
                     REVRVTVLGHTQRGGTPSPFDRVLSTRFGVAAVEMIAQGKMGHMAAVKGEEIVAVPLE
                     QAVARQKLVDPNGELVRVGRSIGVCFGA"
     gene            15866..>16058
                     /locus_tag="PKL17_13240"
     CDS             15866..>16058
                     /locus_tag="PKL17_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK45746.1"
                     /translation="MTTQTSRFAGTDSYVATPDLMVAVNAAVTLGRPLLVKGEPGTGK
                     TVLAEEVARALSKPIIRWHI"
BASE COUNT         3162 a         4755 c         5028 g         3113 t
ORIGIN      
        1 aaagccgtcg accagacccg ccgcacggaa tatcttcagc ggcaggtgtc gcgtctgcgt
       61 gagcttgttg accggctgtc caaaggcggg cgtgaggagc gcgatgcgat gacggcgatc
      121 tcggagtcgc tgcagcggat ggtgcgcacg ctagaggatt tggccaattc gcagcgcagc
      181 aacgccagtg aaggcgagcg gctgcgcaat ttgatggaag tggtgcggcg gggaacgacc
      241 aatcagcgag aacagagcgc cgcgctcgac cgctgtctgg gcgagctggg cacccaggcg
      301 agcgagctac gcggtcagct tagcaagcgc aactagcgcc acgcagcgac tgcattccgt
      361 cgaggaactg ccacagcgcg tgggacgctt gctcggctgc ggacagcact tcgacgcgct
      421 gttcgacggt cttggcgtgc gaaagcaggg cgtcgacttc gacctgacgg tgcaccacgt
      481 cggcttcttc gtggacggaa aagaacgaca ctgcacgcgg atcgcggatg ccgtaaaact
      541 caccgagccc ttcgcgtttc gtcttggcga cgtcgggaat ctgcgactca taagcgtaca
      601 gcgccgccag accttgcaga tagctgccgt ttttggtgag gtcgcggaaa atctcgacgg
      661 acgcttgggt ggcacgggtc ggttcggcgc tttcgacatc gcgacgatcg acgcccaggc
      721 tttcggcaaa acgcagccac agctcggggt ggttgccttc gccttgctct tcctcgataa
      781 ggttttgcaa aagctcttgc cgcagcgcga tgtcgtcaca gttgctgtgc acggcgctga
      841 cgtaacgagg gaatgccttg acttggctga agtactgctt ggaatactca cgcagatcgt
      901 cgaggctgag cgcaccctcg ctccacgcct gataaaacgg gtgtttgagc atcgcgtggc
      961 gctgcaccgc agaatcgagt gcacgcacaa actcgacatc gcgcaaaagg tcaaagcggg
     1021 tcgtcacggt ctcgttcatc ttgggcctct ccctttctat cggatcgacg ggaactcact
     1081 cggttttgga agtcacagcg taccttgcgg tagcttggcg cagcttagcg cagatcatcg
     1141 cgggtcggta agggcttacg ccagaaagcg ggtcagcgcc gcgaacaggg gttctggcgc
     1201 ttcgatgtag gggacatggc cgcagtcggc aagggcaacc agttctgccg aaaggatgcg
     1261 ggcggtttcg gcggcgcttt cgatgggaat cacgtcgtct gtaccgtgga tgaccagcgt
     1321 cggaatgtgc gcaagctcat ggagttttgg acgcagatca aactgcggac ccaggctttt
     1381 ccaaatcgcc tcttcgagtc gctgcttgac caaaaacggc gtcagctcca gcgcccgtcg
     1441 cggattgacg aaatacgatg acaccgcgag cgcaaaacga tggcgacgct gctgttcggg
     1501 cgtggcggtt tcgcgcagac gggcaagctc atcacgaagc gctatgacac ttggccgctg
     1561 catcgcttcg gtcatccgcc tttggtaagt ctgacgaact tctgccgtcg cgggtgccgg
     1621 ggatagcagg atcagttttt caacatgctg cgggtatttt gtcgcgtaca gcatcgacaa
     1681 aagcgcgccc cacgaatagc cgagcagacg cactttatcg accccgatgt agcgtcgcac
     1741 cgcatcgaca tcttcgacgt ggatcgcaac cgacggggga tcttcaccct catcttgcgg
     1801 cgagcggtcg gctccgcgct gatcgtaaaa ataaatcggt cgtccagcgc ccaaatcggc
     1861 cacgcggtcc agctgcgggc gcaagtagcg actgtccgct cccggtccgc cgtgcaggat
     1921 cagcacgggc ggctggtttt taggctgttc gttttgggga aaaacccact caacgtggag
     1981 ctgggctttg gcaaccggaa ggagaaacga acccacgcct agcgtccacg cacaaactga
     2041 atcatcggac gcagataggg cagcgccgcg gcgaggagca gagccgacga caacatcgcg
     2101 agctggtgca ggtgaaccgg cgtgaagagc acgccaaacc gcgaaggcag cgtcaccgct
     2161 tcggtcgcat gggtgtgcag tagcgacagg ggcagcgcga gcgccgtacc caaaaacagc
     2221 atcacaccag cggtccggtt gcgcagcacc gcaaagagtc ccacgcccac gagcacaagc
     2281 agcgcggcct ggccttcttt cggcgcgagc gcgatcagaa tcagcgtcgg caggctcgct
     2341 gcggcgcgcg tcaccgtatg gcgaactttt tcaacccctt gatcgtcggt gactcgcagc
     2401 agttcgcgcc atgacttttg gccttcaaag tgatcggcca gcttctgtcc ctgaaggaac
     2461 agcgcgacga tgccgtgcgt cattccgtaa aacagcagca ccggatcaat cgtgcgtgcc
     2521 gtcaccatgc cccacatcgc cacggtcagg ccggaatagc ccagaccgac ggcaaaccag
     2581 cgcgcccaaa accagtccgc agccagcgca aaaaacgaca ggccataggt caaagacagc
     2641 gccaaaaacg ccggcaccca ggcgcggact tccggttggt ccggcggtgc caagtaaaaa
     2701 agcagcgaca gaagaccgta cagcgccatg tagaaagcga ggcacaggat cgcaaacgct
     2761 ttccgttcac cagaaaaacc aaagggctgg tcaaggagtg acatcgggtc tacctcagct
     2821 cggtttgaga actgctgtca agtatgccaa agctacgccg cggcgaggga caagacacag
     2881 gcaaaacgca gcagggacat ctgttctcct gccacttacg tgcggtggaa gcgccgctcg
     2941 acttccattt tcgacagccg ccacagcact gggcggccat gcggacaata cgggctcaac
     3001 gacacatcat cgagcgccaa tagcagcgct cgtgctttcg cttcatcgag cacatcaccg
     3061 gctcgggtcg cggcatgaca ggccatcgtc gccagcagca gatcatcccg tttttgcagc
     3121 gcgtcggatc gtccgtgttc ttccagctcg tcgagcacct ggcggagcag tctttcgggt
     3181 tcgctgtgca ccgtcgcacc gcgtccgtat tgtccaagat cggggcacga caagagcgcc
     3241 atcgtcttgc cggcaaacgg tttgacctcg aagcccaggt ggtgcagcgc cgcttcaaat
     3301 tcggtgagca gggcgactcg cttgtcgtcg agttccaggt gaatcgggaa aagctgtcgc
     3361 tgcgatgcaa gctgggtctg gccgtgcgcg cgccgcagtc gctcgaaaag cactcgctca
     3421 tgggccgcgt gctggtcgac gatcagcagc tcgttttcgt attcgcacaa aagatacgtc
     3481 gcaaagagct gacccaaata cggcaggtgg gcaagctgga tgcgctgttc atcgtgcggt
     3541 tccgccgtgg gggagggcgg cttatcatct gcctgcatcg cgtcgcgaga atccggaatc
     3601 ggcgcaaata gcttgagcgc ttgctgaagc cggctgcggt gttcggaaag cccactcggg
     3661 cgaggcggtt cgcgcaaaaa cggcacgctg tgggtcgcta cacgttccac cggctgaagc
     3721 tttgctaccg tcgctgcttg cgccaaaaac ggcgcttgcg atgggctttg gggaaccgaa
     3781 atgggcggcg gctggctggg cagcgacatg ggcggtgcaa cagtcgtcgt gccgtagctg
     3841 ggacgcggct ctgcggcaga aagtcccgtc ccaaggacat aggtgcgctc gctctgtgcg
     3901 ggccggctgc gacccaagag ccacggcgcg gtcacgcaga cttcacgaat cgcgcgctgc
     3961 accgccgaaa acaccgcatc ggcattggca atccgcactt cgagcttttg cggatggacg
     4021 ttgacgtcga ccagttccaa atcaagctgc aagtgcagca cgcacagcgg atagcggcct
     4081 ttttcgagca gctcgccgta gcccgtcgtc accgcttgca gcaggctgcg gtcacggaca
     4141 aagcgccgat tgacgagcag aaacacgttg cgcgccgtcg tcgtcgcatc atcgggttca
     4201 cccagatgga cttcgacaga aatgccgggt gcctggcttt cgccttgata cagcaccggg
     4261 gcgccttggc ctttgccacg acttgcgagc gctgctttcg atcgctccag cgagctgcgg
     4321 tgcggcggca ggtcgagcag cacgcgctct ttgtctttgc cgagcgcacg cagacggaaa
     4381 tgcacgcccg gataggccag cgaaagccga atcagcgtct cggagatgtg tcccgtctcc
     4441 gtcgcctcgg tcttcatgaa cttgagccgc gccggcacgt tgaaaaacag atcacgaatt
     4501 tcgatctggg tgccaaccgg acccccgact tctcgactcg ggctttgttt ttggcctgat
     4561 tcggtggtga tccgatacgc agcgacggca tctttggttc gcgtcgtcag ttgcagacgc
     4621 gaaaccgacg ccaccgatgc cagggcttcg ccgcggaaac ccatcgtggt cagtcgaaac
     4681 agatcgtcgg cagaccgcag cttgcttgtc gcatgacgca ggagcgaaag ctcggcgtct
     4741 tcgggattca tgccgcagcc atcgtcggca atgcggacca gcttcagtcc gccttcctcg
     4801 atatcgacgt caatgcgctg cgccccggcg tcgagcgagt tttcgaccag ctctttgacc
     4861 accgaggcgg gcctttctac gacctcacct gcggcgatct gattggtcag ctcctcgggg
     4921 agaagcgcaa tccgtggcat cgactactct tcggtcgtcg gggtctcggg ctcaggccgc
     4981 ggcgccgagt gatgtctcac cgacagcttg gccccgcgtg cccgcatgtc cgccaggtac
     5041 ttttcaacct cgccgcggac atcttgcgtg taatagattt ttccgtcggc aatctcgcgc
     5101 agcgaggtga cggcggactt gttgtcgcac ttgacgaacg gcttgccgcg cttttgcgct
     5161 agctgacggg cccgctcggc ggcgagcatg gtgagtgcga agcgattggg aacccgctcc
     5221 agacagtctt caacggttac gcgagccaaa gtcgttctcc tagaaaagca gaccctgctt
     5281 acccacgcgg gcgctggccc aaaggacagg gaagaacatg tgtatattcc tgcccatcgt
     5341 atcctgtcaa cgcgctcgct gcacttgggt ctttttagac cggggcgggg cctgcggttt
     5401 ccgcgtgggc ggctgcggcg gcagaagtcg cgattgacag gggcggggcg ctgtctttcc
     5461 atgggactta tgcggttgtg ggtgcgtggg ggatttgttt tggcgagcct gcttggggtt
     5521 tcgatgcagc tcgcgtccgc tgtgcccccc gcggcccagg ctgcgccaag cccgccgccc
     5581 ccgatcgtgg cgggacagtt tgcggcagcg cgcggcaccg attttgtgat tggcgatgtg
     5641 ccgtttcgtt ttgtcggggc caacatcaat ccaattcatg gcgacagcga ccggcgggcc
     5701 acccaagcgc tgtttgaagc gatgggcaaa gacgggctca atgtggcgcg ggtctgggcg
     5761 cttggcgaag gcatgcccga cgcgggggag tgggagcaaa aatacgcgct gtttcgcgcc
     5821 ggccccgatc ggtttctcga agacgctttt ttgcagctcg accgcatttt gatggaagcc
     5881 cgccgcgccc atgttcgcgt catcttgacg ctgtcgaaca actgggctga ctacggcggt
     5941 gtgccgatgt acctgcgctg gctgggtctg ccgcctgacg gcatggccaa agaagcgttt
     6001 tatagcgatg aaagggccca tgcgctgttt cgtgctcacg tcggacggct gctcgcgcgg
     6061 acaaacagcc tgaccggcat tcgctacagc gaagacccca ccatttttgc gtgggagctg
     6121 atgaacgaat ccactgtcga gacaccggct ggccgaaagg ctcggctcgc ctgggtgcgc
     6181 gaaatggcgc agtttatcaa gacgtttgac ccccaccaca tggtcgccgc ggggctgtgg
     6241 ggttatgcga tgcgttcaga gcgcgccgat tttgtcgccg ttcacaagct ggcaaacatt
     6301 gattacgtcg acagccacct gtatctgcaa aacagccaag gcaacgtctc actgccgcgt
     6361 ttgtatgatt ttctcgacga ccgcgcccag cttgcgcgcc atgtcatcaa aaagccgctt
     6421 ctgatcggtg aatttggttt tcgcaccgac aaaggaccga cgtatctggg gctttcgcgg
     6481 gcgcgctggt tttccgagct gcttctgcgc cattttcgca accgtggcgg cggcgcgctg
     6541 gtgtggattt acgagccgta cagtggcaag ccgcgagact tcggtatcta catagataag
     6601 tcgacgacag atgatgtccg gcaagcgctg cggacggtgg ccgcacaaat ccgcagtccg
     6661 cttgctgaca atccgcggct cgctccatcg ctgggtgatg cgccgcttta tgcggtggat
     6721 caggtgctgc atggcctttt gcgaccgcgt ttgctgtggc aaaaagccca cggcggagag
     6781 ctctcgttgc ggctcgcacc gtcggagttt cgcgtggcgc acttcgagcg gctcggggtc
     6841 tgggacggtc agccggcggc gcatttttac ggggcggaaa gcggcgacct ggtgtttggt
     6901 ttttcggctc cgcctgcgat ggggcaaaaa gcgctggccg aagtcgaagt ccgcgcgcgg
     6961 ctttcatccg aatggcctgg cgcacaggca ccggcagacg gcggatcgct tgtgcaggtt
     7021 gagctagacg gaatgctgat aggccaatcg accgtgattc ccgatgatgg ccacggcgct
     7081 tggtattcgt ttcggacttc agacccgcgg attttgggca agctgtcgac cggagaacac
     7141 acgctgcggc tgcatgtccc gccgggaccg ctggcccacg gactgtgtct gtacggcgat
     7201 gcgcgcgacc caaagcttgt gaaaaccgac tacgggccgc tgcaaatctt gctacattcg
     7261 gccatcccag cgacgggaaa ttcccaagcg cagacggtga aataggccgt cttaggactt
     7321 gctcgggtcg gggtgatctt cggcttcgat ttcgtcaatc gcttcagcgg gcggcgtgct
     7381 tccgccaatc actttgagca gccccggcag gtagcgatcc atcatcacca ccgcccagta
     7441 cgtcgcgctg cggaccatgg attcggaaag ttcggcgtct tcgacatcga tgctggtctt
     7501 gaagcggacc tcgccatcgg agtagtccat ttcaaagttg ccgatgacca tgccgtagtt
     7561 ggcgcgcgtg atgtattcag cgacggcagc gcgctttttt tcgggggcgc gaatcgggca
     7621 caccgcataa aacaaaaact gctgatcctc ttcgcgggtc tgggcgtaac acgtccagct
     7681 tccgtgctcc ccttgatagc cggtttttag caccgatcga ttttcgagct gcgcaaagtc
     7741 ccaaccatcc tcacgcagaa atgactcaac ggtcttgagc agacgcatgt cgttccattt
     7801 tatcgtgccg gcgggcaaaa aaacggaccc actagcggct cagctgcaaa agtccgatct
     7861 gcaccacggt cgcggcgacg atggcccagc cgacttgtcg caaggcgcgc ggccccggtt
     7921 ttttggccgc cagcccaatc gccagcgcca ccgtcggaat ttgtggccag atgttttcgc
     7981 gcggcaacgt ccaaagctgc gccagtccag cccacaaagc gatgccggcg agcgccagtc
     8041 ccaccgacca gccgagcgca attttgttca tcagaccgcg caccgacagc accgcgacgc
     8101 aaaacagaaa accgagcgac gcccacaggt ggagagacag ggaaagcggc acgctcttgc
     8161 tgacttgcag cggcaccacc cacgacgaca gcgacagcgc gacgagcagc tcggccagcg
     8221 tcgatttttc gtgccgcagc agcataaacg gcacgacgca cagtcccatc gcgagcggca
     8281 gcaaaaatgt ccagccaatc tggggccgaa caaaaagtgc gcccaaaatc ccaagcagcg
     8341 ttcccaagag cagcagcgga aacagccggc tcgccattcg tccctgttct cgctgggccc
     8401 gcgtcccgcg atgtcccatc agcacaagca gcggctcgtg cgaccaaaac agaaaggacg
     8461 cggacagaaa cagcagcagg gtcgccaggc gcggcattcc gtggaggaaa agcgccgtaa
     8521 gaagcggcaa aaaaagctgc ccatacgccc cgtgctcttt ggggaaaagc aggatcatgg
     8581 cgacggaagt tatgtcactt tgaccggcct tgcgttccaa attttggtcg cgtattcacg
     8641 gaccgatcgg tcggacgaaa agtagccggt gcgcgcgatg ttgtggatgg ccatctcgct
     8701 ccacttttgc gggtcgcgga atgcctggtc gacggcttgc tgacacgccg cataggcatc
     8761 gaagtccgcc agcaccaaga atggatcgcc gcggcccggt ttttcctcgg ggggcaatag
     8821 tgcgtcgagc agaggctgat accgcagcgg ttcatcgtag gaaaagaacc ccgaacggat
     8881 caggtcgatc gcgctgtgca gatccggact gcggcggtaa cagtcccacg ggttgtagga
     8941 agcttttttg gccatcactt cgtcggcagt cagaccgaac aggaagaagt tttccggccc
     9001 cacgaagctg cggatttcga cgttggcgcc atccagcgtg cctatcgtca gcgcgccgtt
     9061 catcgacagc ttcatgttgc cggtgccaga tgcctcgaat cccgcggtcg agatttgttc
     9121 cgacagatcc gctgcgggga tgattttttc cgccagcgag acgcggtagt tcgccaaaaa
     9181 cagaaccttc aatttgccgc agacatgggg gtcgtggttt acgacatcag cgacggcatt
     9241 gatcagtcgg atgatcagct tggcgcgggt gtaaccaggg gccgctttgg cgccaaacag
     9301 gaagcagcgc ggcacgatgt cgagcgacgg attttgtttg agccgctgat acagcgaaat
     9361 gatgtgcagg acattgagca gctgccgttt gtattcgtgc agacgcttga cctggacatc
     9421 gaacagcgcg tgcggatcga cggtgacgtg gttgtggcga gcgatgtatt cgcacagcct
     9481 ttgtttgttg cggagcttta tcgcctgcac ttcggcctgg aagtcgggat ctttggcaaa
     9541 cggcacaagc cgttccagct gttccagttc cgtcggccag ctatcgccaa ttcgccgcgt
     9601 gatcgcttcg gccagttccg gattggcccc caaaagccag cgccgcggcg tgaccccgtt
     9661 tgtcacattg atgaagcggt cgggaaacat cccggcaaag tcgggcagaa ggtgttcgcg
     9721 caaaagccgg ctgtgcagct cggcgacgcc gttgactttg tgcgatccca gcgtcgccag
     9781 atacgccatg cgcacgccct tgaacggctg ctcttgcaca agtgacatcc gctgcaggcg
     9841 ttcctgatcg gacgggttgt tggtccagac ctggcgcaaa aagcggtggt ttacttcgta
     9901 gatgatttgc agatggcgcg gcagaagctt gccgagaagc tcgacgctcc agacttccag
     9961 cgcttcgggc aaaagcgtgt gattggtgta cgcaaacacg ctttcggtaa gctgccaggc
    10021 ggtgtcccat ggcagctcgt atttgtcgat gagcagccgc agaagttcga cgatcgcaat
    10081 cgacggatgg gtgtcgttta gctggatcgc gaccttttcc ggcagcatca gaagccgctc
    10141 gcggatcgcc tgcggatcgt gacgattttt cgcatggtcg aggtggcgac gtagcagatc
    10201 ggcaatcgcg cacgcgacaa agaaatactg ctgcttgagc cgcagttctt tcccaacgac
    10261 gagcagatcg tttgggtaga gcactttgct gatcgactcg gagttgtttt tgtcgaacac
    10321 cgcgcgctcg tagtcgccgg cgttgaacac cgtcaggtca aactcttcgg atgcgcgggc
    10381 gctccacagc cgcagcgtgt tgaccgtgtc gtttttgtat ccgacgatcg gcatgtcaaa
    10441 cggcacgccg agcaccgacg tggtatcgac ccagcgaacc tgcgggtggc cgttgccgtg
    10501 gacttcggtg tggccataaa agtggaccgg ctgggtaaat tccggacgcg cgaccatcca
    10561 cgggctgccg tagcggagcc actcttcggg tcgttcgatc tgggcgccgc cgcggatcac
    10621 ttgatcgaaa atgccaaact cgtagcggat gccgtagccg taagccggca gtcccagcgt
    10681 cgccatgctg tcgaggtaac aagcggccag tcgtcccaaa ccaccgttgc caagtccagc
    10741 gtcgcgttct tgatcaagca gatcgccaag gtgcagcccc agtcgcccgg tgacgctctg
    10801 cgccttgtcg taaaggccca tgctaagcag gttgttttgc agcgcccgcc cgaggagaaa
    10861 ttccgccgac aagtaataga cgcgcttggg atcttgtttg cgataggcgt ctttggtgcg
    10921 catccacttg gccatcacgc gatcgcggac ggtgtgggcc agcgccagat agcggtcgtg
    10981 ctgcgtcgcg tgggtgtcgt ctttgcccag cgtaaagtgc aggtgatcga ggagcccgcg
    11041 ggaaaagccc tcctcggtgt agcttgtgcg gtcgtcctcg acttggatga tcggcggaca
    11101 aggggtggtg gtagcgccgt cgcttgacat agccgcatcc tagccgagac cgtgggccgc
    11161 cggtaagctg gctgcccgcg gtttgtcggc tgcggcggcg tttacgactg ggtttcttcg
    11221 gttttggcca agatgtcggg atgtgcgtac acgccgccag ccagcgagga gacttgatag
    11281 cgatgctggg ccagcacccg cgcggcaaaa tagctggtct tgccatgcgc acagacggtg
    11341 accaccggca cgtccggact gagcatcgcc agcttgtgcc gaagcgcagg cagcggaatg
    11401 ttgactgctc cagcaacggg atgggccgcc gaaacggctg cgctacgaac gtcgacgatc
    11461 tgcgcggttc ccaaggcagg caaggactgg acggggttta caagaccatc gcgtcggttt
    11521 gtcgccgcaa agccagcaat gttgatgaca tctttggcgc tgccaaacgg cggggcatac
    11581 gcaagctcca agtgcaccaa gtcgtcaatc gtcagctctc cgcgcagtgc cgtcgccagc
    11641 acatccagtc gtttgtcgac gccatcggcc ccatacgcct gcgccccgag cacgcggccc
    11701 gtcgtctttt cccacagcag cttgagcgtg accgtctgcg cgccaggaaa atacgaagcg
    11761 tgggacgacg cggataccag cgtgctttcg tagggccgtc ccagctcttg gagccgtttt
    11821 tccgaaagcc cggtgattcc cgccgccaga tgaaacacgc gcacgatggc tgtccccagc
    11881 gatccgggat agggggccgc agcgtcgccg cgcacgatgt gatcggcgca gactcggcct
    11941 tggcgattgg ccggcccacc aagcgggacg ttggacacgc cgccgaccag tggatcgggc
    12001 gttgcacaga catctccgac ggcatacaca tcggggtcgc tcgtctgcat aaacgaattg
    12061 accgcaatgt gtccgcgtgg cgtaagcaaa agtcccgctt tttgggccag cgtcgattcg
    12121 ggacgaacgc caatcgacag aaccactgca ccgacggtca ggcgttctcc cgttttggta
    12181 atagcggtca ctttgtgtcc cggtccggct tcaaacgatg tgatcgccgt cgacagtcgc
    12241 agatcgatgc ggtggtggcg cagctcgtgc tcgatgggtc gcgtcatttc tgcatcgagc
    12301 agcggcagca cctgcggttg aagctcgacc agatgaacgt gcttgcccag gtgtgcaagc
    12361 tgttccgcca tttccagtcc gatgaagccg gcgccaatca ccagcacgga atggacgttg
    12421 tgcagcgcgg tcttgatccg atccatgtcg accaagttgc gcagcgtcag aatctgcggg
    12481 tgatcgattc ccggcagcgg tggacgcagc ggtgccgcac ctggggccaa aatcagcttg
    12541 tcgtagggca gcgtggactg ctgaccggac atcctatcga ccaccgtcac ggtgcgcgcc
    12601 gttcgatcga gatcggtcgc ttccgtattg gcgagcacgc gcaggttcag catcgcatgc
    12661 agcgacgcgg gcgtctgcac cgagagcgca tcgcgggagg cgatttcacc gccaatgtga
    12721 tagggcaggc cgcagtttgc aaagcttacg tcggggccgc gctcgataag cgtgatgtcg
    12781 gcaaactcat cgagccgccg aagtcgcgcc gctgccgatg cccccccggc gacaccgccc
    12841 acgatcacca cacgccgcga aactgaaccg gtattcatga cagaaaccca agctcctttg
    12901 tctgggtaga gccagggaaa gcaaaagagg attcagtgtt ttttcaaaaa aattcatttt
    12961 ccgtcgctga taaagaaaaa taaggcatgg atttgcagtc gatttcacgc gccaaagcgg
    13021 gtgacaaagc ggcgctcggt cagcttctcg aagagatcgc ttcgccggtg tatcgcttca
    13081 gccggcggat gtgtggcagc agtgccgacg ctgaagacgt gacccaagaa acgctgcttt
    13141 cggcggcgca gaacctgtcc cagttcgccg gtcaggcatc gtttcaaagc tgggtgtttg
    13201 cgatcgcgcg cagtgcttgt tcccatcgct atcgcggcaa aaaaaaccag ccgcatcaga
    13261 cggaagactc gcttttggcg ctggccgcgg cgacacccga tccggaaacc gcagtccttt
    13321 cccatgagca cgctgcggtg gtgaaaagcg gtctgggaat gctcccgccc gatcagcgcg
    13381 aagtgctgtg gctgcgcgat gtcgaaggtc tgagcggaca ggagaccgcc gacgcgctgg
    13441 gcctgtcgat cgaagcggtc aagagtcggc tgcaccgcgc ccgccagatg ttgcgacagc
    13501 ttgtgtcgac caaacttcaa ctggcgctgg aacagccgct ttcatcgtgt cccgatgtcg
    13561 tcgaaatgat gtcgcgcaag ctcgaaggcg atttggcgat ggcagactgt gcccaaatcg
    13621 aaaaacacgt tgcgaactgt ccgcgctgtc acggtcgctg cgatgaaatg cgccaggtct
    13681 ttgtgctgtg tcgacaggct gcgacggaaa gtgcgccgag cgaagtccga atcaagctgc
    13741 ggcgcttgct caatgcgcta tagcttgatg gatatatgaa tcatttaata tgcttcttat
    13801 atggaataag aatcatataa aatgcctttt atctctgaat aagaggagta taaaatgttt
    13861 cttatctggt gccgaggatc acggtccagc gcgtggcggt cacggcggca ccgaggaagc
    13921 agacttcggg gtgaagcaag ttgccgcggc tggtcttgag gagcgctgcg acggcttcat
    13981 cgggggactg cgggcctttg gtcaaacagc cgacgctgcg gcctttgaaa ccggctttgg
    14041 cgacgcggct tgcgatgctg tcgccgctcg ggctggcggt gccaaagtag tccttttggg
    14101 ccatgtccgc ggagtgttct tgcgcggcgc tcgacagcgt atcggacagc tccagaagcg
    14161 gcaggttggc gcggcggcgt tcctcgttga cgtgcttgag aaccgcaaac cgcagctctt
    14221 tgacatcggt catctgaagt gacggcggca tccccaggac aaacgtccac agcccgtcgc
    14281 tgaccccgac gcccaagtgt tgataggttt ccgacatcaa gtgccgctgg tactgcgcgt
    14341 gcgccagcca ctcgcggatg acgccgtcgg gctgctcggc tccgtcggcg accaggactt
    14401 cgcagcgacc ttcgtagccg gcgcgccgca cctgtcctga caccggatcg ccttccggcg
    14461 tcgcttcgcc catgtagccg cgcttgttca tgtcttcgga atgggcctgc gcgacctgaa
    14521 atagctgcgg cacacccgac agagggtgca gacccagctt gccacgctct tgattgacca
    14581 gcgtgacggc gcgctcgagc gcttttggat cgggcccacc accctgcttg ggtcgattga
    14641 gtccgagcat ctggtttagg tcgaggttta cttcgattgg cttttgcggc atcccggccc
    14701 cccgtgtgcg aaaacgaacc aaaaaacggt tcatcatacc acgggacttc gggtagcttc
    14761 cgacgctaga atgccccctc atgagcaagg tgaagacaat cgcgatctcg accggtggtg
    14821 gggactgtcc gggacttaac gcggtcatcc gggcagccgc aaagagcgcc attctccagc
    14881 acggctggcg cgtcctcggc atcgaggaag gctataacgg gctgatctgg ccagaccgga
    14941 tcaaagagct gacgttgtgg aacattcgcg gcatcttgcc gaggggcggc accatcctgg
    15001 ggacgaccaa ccgttcgaac ccgttcgcga tcaagatcgt cgaaaacggc accgaggtga
    15061 cgcgcgatct gtccgattcg gtgattcaaa atgcgcgctc gctcgggatt gacgcgctga
    15121 tcaacatcgg cggcgacggc acccaaacga ttgggctgaa gcttcacaaa aaaggtctgc
    15181 cggtcgtcgg ggtgccaaag acgatcgaca acgatctgtc cgcgacggaa acgacgttcg
    15241 gttttgatac ggcggtgcat gccgcgaccg acgcgatgga ccggctgcac acgacggcag
    15301 aatcgcacaa gcggatcatg ctggtcgaag tgatggggcg cgacgcgggc tggattgcgc
    15361 tgcacgctgg catcgccggt ggatcgaacg tgatcttgct gccggaaatc ccgtttacca
    15421 tcgagcgcgt ctgtgaacac atccgcaagc gcgagttttc ggggcggcgg gcgacgatca
    15481 tcatggtggc cgaaggaatc cacgttccca aagacctgcc ggcggacttg atcgaagaat
    15541 ggaaccgaac gcctcggcgc ggtgctgtca gtcacctgat tggcgaagcc gtgagccgtc
    15601 acgctggacg cgaagtgcgg gtgacggtgc tgggtcatac ccagcgcggc ggtacgccat
    15661 cgccgtttga ccgggtgctg tcgactcgtt ttggcgtcgc tgccgtcgag atgattgcgc
    15721 aaggcaagat gggtcacatg gcggcggtca aaggcgaaga aatcgtcgcg gtgccgctcg
    15781 agcaagcggt ggctcggcaa aagctcgtcg atccaaacgg tgagcttgtg cgcgtcggac
    15841 gctcgattgg ggtatgtttc ggcgcatgac gactcagact tcccggtttg ccgggaccga
    15901 tagctacgtc gcaacgccgg atttgatggt ggcggtcaat gccgcggtca cgctcggacg
    15961 gccactgctc gtcaagggag agccggggac gggcaagacc gtgctcgccg aagaggtcgc
    16021 gcgcgcgctt agcaaaccga tcattcgctg gcacatca
//
LOCUS       DAORGN010000116        15830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1516_length_15830_cov_5.650016, whole genome shotgun sequence.
ACCESSION   DAORGN010000116 DAORGN010000000
VERSION     DAORGN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15830
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_15830_cov_5.650016"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="PKL17_13245"
     CDS             complement(<1..2057)
                     /locus_tag="PKL17_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNK45747.1"
                     /translation="MLLQFAAKKWTPCTGAVCDIVKKATPSKSQKKAKGKTISHDLAA
                     PSVAPAVVPEVIEAVHVDPPTVVEAEVVDPQPAAATTAESATVSETSETASAQAAAPV
                     SPAPQAESHAGETSDDTKGGGPSGPSGPSAPRMIVCRNPATGEVLGEVPAMDAAEVGR
                     RLIAARIAQREWQKTSFAERRRVLRRLLDAILDRADELCRMLSAEAGKTLQNAMMGEI
                     FPICEKIRYTLNNGEADLQPEKISSGLLLHKTAQIEFPPLGVIGVICPWNFPLQNILG
                     PTIPALFSGNAVLCKVSEWSSHSAPRVQAILADVLSSCGHSPDLVQIITGFGETGAAL
                     VRSGVDKIFFTGSVPNGRRVLEGSVQNLTPVVLELGGKDPLIVCDDADLSQAVAGALA
                     GVFICSGQMCLAAERIYVMDGIYDRFIERVVSEAQQLRQGDPLGGNGADIDVGAMTMP
                     HQVEIIEKLVADAVQKGAKVLVGGVRRPGGNFFAPTVLVDVDHTMDIMREETFGPVMA
                     IMRVRDDQHAIELANDSVFGLGSTVFSKDQARAKRLADRLIAGSTCINDYGLAYMVQG
                     LPFGGVRNSGFGRLNGREGLRACCNPKSVVGDRFPIHIPSKIYPVKPGDYEMVRAAVN
                     LIYRPLDLTGIKARAQAILASDEFAEAVRAQEAYFQTRGIQAVPAVIVNQRHLIQGGQ
                     PVEV"
     gene            2111..3169
                     /gene="tsaD"
                     /locus_tag="PKL17_13250"
     CDS             2111..3169
                     /gene="tsaD"
                     /locus_tag="PKL17_13250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNK45748.1"
                     /translation="MRVLALESSCDETACAIVEATHEYGHPQHSLRVRASVIASQVAI
                     HARFGGVVPEVAARHHIQSLIPVIDEALLQAELSLHDISALAVTRGPGLIGALLVAVQ
                     AGKALAFARGLPLVGVNHLEGHLLSAYLSETERDGATPHVRPPLPHLGLLVSGGHSSL
                     VLVRDFGDYQVLGTTCDDAAGEAFDKVGKLLGLGYPAGPLIDKLSTMGDPKAVPMPRA
                     MLGAKRGLSMSFSGLKTAVSAHLHQHGQPQGEQALSDLCASFQAAVIEQLVRKTALAL
                     DLHKLPAVVLSGGVACNRALRASVAALCEKRGLSFFVAPPRLCSDNAAMIAAAGYYRL
                     WRGERADLSLNAVANLPL"
     gene            3144..4049
                     /gene="rsmA"
                     /locus_tag="PKL17_13255"
     CDS             3144..4049
                     /gene="rsmA"
                     /locus_tag="PKL17_13255"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNK45749.1"
                     /translation="MPLPTFPSDTGATAPQTPDQPLVLDARTLLRRYDLHAKKSWGQN
                     FLVDERSYSAIVAACRLAKDDVAVEIGAGLGTLTSRLLATGATVIAVERERDMCSVLR
                     SELGAEPRFVLREENALHVDFDVLAAQYGRRLLLVGNLPYQIASPLIFRFLAMRSSLR
                     RIVIMIQREMADRVLAPPGSRDSNALGVQVQMVCAVRRVCQVGRGAFLPPPRVDSSVI
                     SLEPLPGTAVPLRDLDTFHHVVRAAFGQRRKTLRNALSAVFPDDVMARFSACGVDLSR
                     RGETLSLAEFAALSDAVPPPSSHPA"
     gene            4065..5153
                     /locus_tag="PKL17_13260"
     CDS             4065..5153
                     /locus_tag="PKL17_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45750.1"
                     /translation="MSRRFLGLSVLLLIVTCAVFFYRHVQHAKPKGLVLSPSVAVPDL
                     GRTGNPAGLGSTQVVTGKTPSSHAPIASDVVATGSFGGGVGQFGRKRQAESSPEGPMA
                     ISAAPDGSVLIVDQVNRRVARYKDGRFVSQIPIGGDTVQDLAIGAEGRVVLLDRFVDR
                     GILVYGPDGKLINDVPLPSKLDLGSLTGVFADEHGIYVEREHGQVTQVADANGNRIEG
                     SKELSGRPSRDGQLVLSAALLERQSGQVLIRAHDRNRATLVFEKVVQLPTPVLHLVML
                     DSDRSGRIYFAAVTAIEDQAPPYALSDEALFAVRLGRDGSERGRLKLPPLHGSDESFR
                     PVTVDDGGNLLVMQSSETGLSVVRYAFP"
     gene            5190..5990
                     /gene="lipB"
                     /locus_tag="PKL17_13265"
     CDS             5190..5990
                     /gene="lipB"
                     /locus_tag="PKL17_13265"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNK45751.1"
                     /translation="MTAIPIEDFAAAAKLPPVDGQAVFLGRMGYGVAWKLQQLVVEAR
                     QNGQIPDTLLLCEHDDVITLGRRQTGPANVLLRRFPIFEIERGGDATYHGPGQLVGYP
                     ICFLRPGPDPAQPFGEQDLHGFLRALEEGLLHLLRLCGVACDRKPKYTGVWTADQTGK
                     LVSLGVAVRRWVTSHGFALNVTTDLSRFLAINPCGMSAQVMTSLAALGVQWDGKPVSV
                     DALIAPCAASLGASLGRRFGLRSVEEAGLADLLRQAKQTPELPAFSPG"
     gene            complement(5977..6813)
                     /locus_tag="PKL17_13270"
     CDS             complement(5977..6813)
                     /locus_tag="PKL17_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNK45752.1"
                     /translation="MKTLAEHLAAEPFELVLSSGFFGFFAHTGVVLALEEAGLTPTQL
                     GGSSAGALVAGLWGAGLPATVLRDTLLGLSRSAFWDVDPLWGLPFYLRRGKLRDIAQR
                     LGIGRLADESSGPGLLRGQAFDYILRGLLSQVSVETFDDCAIPVRLAAFALDTLQTEV
                     LDEGDLTLAIRASCCFPGMFQPVKIGDRRYLDGGIADRPGISTATPGARLLFHHLPTH
                     SPWRKVIRVQNEVPDWPDLHLLHEPSLPKLSPFKLEPGQRALQLAYEMTLRRLQEPVS
                     QD"
     gene            complement(6861..7940)
                     /locus_tag="PKL17_13275"
     CDS             complement(6861..7940)
                     /locus_tag="PKL17_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45753.1"
                     /translation="MNDKTTAFDSQAELTVPSVIYRWDLDKTYLRTEFDTFMSLVRTA
                     FEDAEDKRTVPGAATLLRELRKSSVGRARICFISGSPRQMRKVLTEKLRLDGVEFDEF
                     VLKPNLRNMLTGRFRALREQVGYKLPALLAGRAGVTAETREVCFGDDAESDALIYSLY
                     GDLLAGRIDRSLLEQILRAAGTYEDDAARTVSIFESLPHSEAVERIFIHLDRRSPPGK
                     FDRYGSRLVAIYNYFQAALTLFADHRIEAAAVVRVAVEMVDRHSYTIEALRNSLQDLL
                     RRRHIAIETVNDLGRAIADGAGETDLLPALGTLPGTRDLLRTLASGLRDLGPLTEAEL
                     VPKPSDAPIDYLAALADLPKRSKRK"
     gene            complement(7949..9097)
                     /gene="plsX"
                     /locus_tag="PKL17_13280"
     CDS             complement(7949..9097)
                     /gene="plsX"
                     /locus_tag="PKL17_13280"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNK45754.1"
                     /translation="MSARDTGSHAVTEKGDKTDSHERVAKQPITVVVDAMGGDHAPEE
                     VVKAACALTIEKPDKDAGAPLHLLLVGDAPRLHQLLMQQRYDAERLAIHHASQVITME
                     ETPKDALLHKPDSSIAVAARLCAEGRASALVSAGNTGAAVLSCLQYWKRLPGIRRCAL
                     AAVYPTEVRRGEKDDPFSLILDVGATLDVTADDLVSFAIMGSAYAARISKNPKPKVAL
                     LSNGAEAGKGPKEVVEAHAKLKSLHSVDFIGNVEGVDIPRGVADVVVCSGFVGNVVLK
                     MLEGVSETVVRLAHYAYKEKLMWRAGLAMLSGGIGKLKELTDWKQYGGAPLLGFDRLL
                     IKAHGRSHAPAIQNAIKVAKKSVTSGLLYDIERGLIEAANLKAQSESK"
     gene            9404..9607
                     /locus_tag="PKL17_13285"
     CDS             9404..9607
                     /locus_tag="PKL17_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45755.1"
                     /translation="MSEDDIIYWQDVLESVQAGRTANLKCPFCQQGDIEVKKRERVTT
                     VRCTAKGCGHFIEGRFSAEDVAE"
     gene            9820..10221
                     /locus_tag="PKL17_13290"
     CDS             9820..10221
                     /locus_tag="PKL17_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNK45756.1"
                     /translation="MARNFLGKGWRYPVMIDRHGGMAQSSLDDLVRESILIILGTAPG
                     ERVMRPYFGCDIHELLFAPNNVNTAGLAAHYVVEALEKWEPRVEDVFADANPDPNEPH
                     KLIIDIKYKIRGQNVTRNLVYPFYLRRSDQE"
     gene            10218..13229
                     /locus_tag="PKL17_13295"
     CDS             10218..13229
                     /locus_tag="PKL17_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNK45757.1"
                     /translation="MIPTPKLDDRTYADIIAEAIRLIPRYCPEWTNHNPSDPGITILE
                     LCSWMTELILYRLNKVPEKNYLTFLDMLGIKLQPAQPARALLTFKLADGVERQTIRRG
                     LQVSTPQADEEDAVLFETQRDLTVISANLDRCFSYFDEAYSDNSPYLQGERTEGFLSF
                     AGAERVDRFIYLGDERLIGVNDSTVLRLRMTAPEHGGRDLARLLSWEYWNGRRWRELT
                     LSQLEVERGEVIFQGVVDMQKTTVNGLETYWLRGRLAEVPRSPAETEVDTVRGRIEVT
                     GEGILPDQAFANLDNNVFIALDLGKNVHPFGTEPKPDHVLYLASQEALSQVGVQIEID
                     FALSDPQIAPPPKPSDDLILSWEYFDGKKWRILGKSTPSGKPPQGWEQSVLGFVDATN
                     AFTKSGTVRFNRPPDLQPGEVTGEPNYWIRVRIEQGDFGVPGAYMLDGDKWVWRDERP
                     LKPPAFKSIGIRYQTDLDHLKYFVSYNDFRYRDHSEEEKNEYRPFQPFQAIPDEGAAL
                     YLGFDHRLPNETMSLYFQFVESTVVEQDFAHAEHLTQYYEKRKTIWEPEQRISWEYYN
                     GRAWVTLVVADGTKNFTESGFIDFVGPDDMDKTLKFTEDRFWIRARLEMGGYIKPPRV
                     RRILQNTVEVANVRTVRDEILGSSDGTPLQNYTLLHGPLLGGQVIEVREREEPLGEEV
                     EELGEHAVRMADDREGGYMVRWREVESFFESGPKSRHYIIDPLTRQVRFGDGIRGMVP
                     PVSRNTVIAAEYRVGGGSKGNVNAFTLTSLTRAVPYIEKVYNVLPAVGGADAETVEQA
                     KERAPLQIKSRDRAVTSEDFETLALRSTTSIARAKAMSSDDRAGHVHLVIIPRGDEKN
                     LDLTRKLVPPPELCRFVRNYLDERRLITTILDVTKPDYVEISLKITLIRRTVGQSERL
                     RTEIEQRLRRYLHPLVGGKDGKGWPFGRPVYRTDLVHMVEDIPGIEAIESIQIYDEDR
                     RINVDSVRLKDDELPHVVNVAVIERVRQEMI"
     gene            13229..14971
                     /locus_tag="PKL17_13300"
     CDS             13229..14971
                     /locus_tag="PKL17_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK45758.1"
                     /translation="MLHEREPMQRRLVRVPDVVGLSLQKALLLVQHAGLKVGAVLFKE
                     SYEEKDTILEQKPQRGQMVYASDDVTLWISRESYIKWLPAIYQRSDATGRNYVRDVLW
                     IIQHLFGSIESVLDGIHLYFDSYETPEQFLPWLAAWSAMILEPDWPIAKKRRLIKKAM
                     ELYRLRGTVRGLKLFISLFTGFEPHLAENEWPFNGFRIGVSSTVGIDTVILPPVDKAH
                     SFVCVMPHKFKDVSAQSIIRLHEIIEMEKPSHSTYMLKFEDPRQAVEEREFYRIGVSS
                     GVNIGQEVVTPLPISEETGEPDYPKEPVMPPPPPNPSVYELFGTKKRQLPAITGAAKA
                     DNAPVEGREITTSKYGFEGAREFRLDDIMKELLARAGLEQSVDVSVDSSAGSGAAVNP
                     AVVELEKNDISAGGDEALLESTLESSGPSEKVLYSTDQPKAPAEPATHAESAASAEGE
                     ESSKTPVTGAAPAADEDGDIPRAGLGATVEGGIRGRKPKIDIDVSVEEVDGDAVEEIS
                     ADDLISEVDKTSSETSDSKHSDVSPVKTSASVEGSVKTGSPRVEIEVEKREVIRDEGS
                     APSGSDKPEDGSKK"
     gene            15007..>15830
                     /locus_tag="PKL17_13305"
     CDS             15007..>15830
                     /locus_tag="PKL17_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45759.1"
                     /translation="MATESESKVKGFKRINFFKGFLTTEHDWNDAERYHIEKRRLHNK
                     MMHAPGVVFGFSGDLKVTARPRGDLSVEIQPGYAVDGQGRDLMLWDAEIKTLVLEEYK
                     LPQTIYIVLRYYEQETDFIAYKENAAYKGHRRILEGCKVEISQTVPDIREEVELARIL
                     LDKNATRIRDARDPNEPKANEIDLRFVPRAGVAGASMDPVLKIRLALLLERLRRLLLA
                     MARDNKVETAHDALAGVIAAQTLHVSDLLDNRNGHEVLNMLVQLQDELSKDVEVNHP"
BASE COUNT         3033 a         4666 c         5018 g         3113 t
ORIGIN      
        1 acctccaccg gctggccgcc ctggatcagg tggcgctgat tgacgatcac cgccggcacg
       61 gcctggatgc cgcgcgtctg gaaatacgcc tcctgcgcgc gcacggcctc ggcgaactcg
      121 tccgaggcga ggatggcctg cgcccgcgcc ttgatgccgg tcagatcaag cggcctgtag
      181 atcaggttga ccgcggcgcg caccatttcg tagtcgccag gttttaccgg ataaattttc
      241 gacggaatgt ggattgggaa gcggtcgccc accaccgact tggggttgca gcaggcgcgc
      301 agcccttcgc gaccgtttag tcgaccaaag cccgagttgc gcacgccgcc aaagggcaga
      361 ccttgcacca tgtaggccag cccgtagtcg ttgatgcagg tcgatccggc aatcagtcgg
      421 tccgccagcc gcttggcgcg cgcttgatct ttgctaaaca ccgtcgaacc gagcccgaac
      481 acgctgtcgt tggccagctc gatggcgtgc tggtcgtcgc gcacccgcat aatcgccatg
      541 accgggccga acgtctcttc gcgcatgatg tccatcgtgt ggtcgacatc gacgagcacc
      601 gtcggcgcaa agaagttgcc gcccggtcga cgcactccac cgaccaacac tttggcccct
      661 ttttgcaccg catcggcgac cagcttttcg atgatttcaa cctgatgcgg catggtcatc
      721 gcgccaacgt cgatgtcggc cccattgcca ccgagcggat cgccttgccg aagctgctgc
      781 gcttcggaca ccacgcgctc aatgaagcga tcgtaaatgc catccatgac gtagatccgc
      841 tcggcggcca ggcacatttg tcccgagcag atgaacacgc cagccagcgc cccggcgacc
      901 gcctgcgaca gatcggcatc gtcgcacacg atgagcgggt ctttgcctcc cagttccagc
      961 accaccgggg tcaggttctg cacgctgcct tccagcacgc gccgaccatt cggcaccgat
     1021 ccggtgaaaa atatcttgtc gacacccgag cggaccagcg ccgccccggt ttcaccaaac
     1081 ccggtgatga tctgcaccaa atccggcgag tggccacacg atgacagcac gtccgcaaga
     1141 atcgcttgca cgcgcggcgc cgaatggctt gaccactccg acactttgca cagcaccgcg
     1201 ttgcccgaaa acagcgccgg aatcgtcggt cccaggatgt tctgcagcgg gaaattccac
     1261 ggacagatga caccaatcac accgagcggt ggaaattcga tctgcgccgt cttgtgcaag
     1321 agcagtcccg acgagatttt ttccggctga agatccgctt cgccgttgtt gagcgtgtag
     1381 cggatctttt cgcagatggg gaatatctct cccatcatcg cgttttgcag cgtcttgccg
     1441 gcttccgcgg acagcatccg gcacagctca tcggcgcggt cgaggatggc atcgagcagc
     1501 cggcgcagta cgcgacgacg ctcggcaaag ctggtctttt gccactcacg ctgcgcgatg
     1561 cgtgcggcga tgagcctgcg tcccacctcg gcggcatcca tggctggcac ttcgcccagc
     1621 acttcacccg tcgctggatt gcggcacaca atcatgcgcg gagccgatgg tcccgatgga
     1681 ccggacggcc caccgccctt tgtgtcgtcg cttgtttccc ccgcgtggct ttccgcttgg
     1741 ggggccggcg aaacgggtgc tgccgcctgc gcactcgcgg tttcactggt ttctgaaacc
     1801 gtcgcagatt cggcagtcgt cgcggcggct ggctgcggat cgacgacctc cgcctcgacc
     1861 accgtggggg ggtcgacgtg gaccgcctcg atcacttcgg gcactacagc cggcgcgacg
     1921 gatggcgctg ccaggtcatg gcttatcgtc ttgcccttgg cttttttctg cgacttcgat
     1981 ggagtggctt ttttcacaat gtcacacacc gcccccgtgc agggcgtcca ctttttggcg
     2041 gcgaactgta gcagcaaggc ctacaaacga aaagcgcaaa ggcggacgcc cttctgctag
     2101 attccccgcc gtgagagtcc tggctctgga gtcgtcctgc gatgagaccg cgtgtgcgat
     2161 cgtcgaagcg acgcacgaat atggtcatcc gcaacacagc ctgcgcgtcc gcgcgagcgt
     2221 gattgcgtcg caggtggcga tccatgcgcg ctttggcggc gtggttcccg aagtcgcggc
     2281 gcgtcaccac atccaaagcc tgattccggt gatcgacgaa gcgcttctgc aggccgagct
     2341 ttcgctccac gacatttcgg cgctggcggt gacgcgggga ccgggactga ttggtgcgct
     2401 gctcgtcgcg gtgcaggctg gaaaagcgct ggcgtttgcg cgcggtctgc cgctcgtcgg
     2461 tgtcaatcac ctggaaggcc atctgctttc ggcgtatctg tccgaaacgg agcgcgacgg
     2521 ggcaacgccg catgtgcgac ctccgctgcc gcatcttggg ctgctggttt ccggcggtca
     2581 ttcgtcgctg gtgctggtgc gtgactttgg tgattaccag gtgctgggga ccacctgcga
     2641 cgatgccgcg ggggaagcgt ttgacaaagt gggcaagctg cttgggctcg gctatccggc
     2701 gggaccgctg attgacaagc tgtcgacgat gggcgatccg aaagcggtgc cgatgccacg
     2761 ggcgatgctc ggggccaagc gcggtctttc gatgagcttt tcggggctca aaaccgccgt
     2821 ctctgcgcac ttgcatcagc acggccaacc gcaaggcgag caggcgcttt cggacctgtg
     2881 tgcgtcgttt caggccgcgg tgatagagca gcttgtccgc aagaccgcgc tggcgctcga
     2941 cctgcacaag ctgccggcgg tggtgctgtc gggcggcgtg gcgtgcaatc gggcactgcg
     3001 ggcatccgtc gctgcgctct gtgaaaagcg cggtctatcg tttttcgtcg cgccgcccag
     3061 gctgtgtagc gacaatgccg cgatgatcgc ggcggctgga tattaccgtc tgtggcgcgg
     3121 tgaacgcgcc gacttgagcc tcaatgccgt tgccaacctt cccctctgat acgggcgcga
     3181 ctgcgcccca aaccccggat cagccgctcg tgctcgacgc gcggacactt ttgcgtcgct
     3241 acgatctgca tgccaaaaaa agctggggcc agaacttcct ggtcgatgaa cggagctatt
     3301 ccgctatcgt ggcggcctgc cggcttgcca aagacgatgt cgccgtcgaa attggcgcgg
     3361 gacttgggac gctgaccagt cgacttttgg cgaccggggc caccgtgatt gctgtcgaac
     3421 gcgagcgaga catgtgctcg gttttgcgca gcgaactggg cgccgagccg cgctttgtgc
     3481 tgcgcgaaga aaatgcgctg catgtcgact tcgatgtgct cgcggcgcag tacggtcgtc
     3541 ggcttttgct ggtcggaaat ctgccctatc agatcgcgtc cccgctgatt tttcgctttc
     3601 ttgcgatgcg atcgtcgctg cgacggatcg tgatcatgat ccagcgcgaa atggccgatc
     3661 gggtgctcgc gccgccgggc agtcgggact caaacgcgct cggtgtgcag gtgcagatgg
     3721 tgtgcgcggt gcgtcgggtt tgtcaggtcg ggcgcggggc gtttttgccg ccgccgcggg
     3781 tcgattcgtc ggtgatttcg ctcgaaccac tgccgggaac tgcggtgccg cttcgcgacc
     3841 tcgatacgtt tcaccatgtg gtgcgcgctg cgtttggcca gcgacgcaag accctgcgaa
     3901 atgccttgtc ggcggtgttt cctgatgatg tgatggcgcg cttttcggcc tgtggggtgg
     3961 atctctcgcg gcggggcgag acgctgtcgc tggccgagtt tgccgcgctg tccgatgcgg
     4021 tgccgccgcc gtcttcccat cccgcttgac ggagcttgcc gctgatgagt cgtcgctttc
     4081 tcggtttgtc ggttctgctg ctcatcgtga cctgcgcggt gtttttctat cggcacgtcc
     4141 agcacgccaa gccaaagggt cttgtgctca gcccgtcggt ggctgtgcct gatttggggc
     4201 gaaccgggaa tccggctggg ctgggatcga cgcaggtggt gacgggcaaa acgccgtcgt
     4261 cgcatgcccc gatcgcgagc gatgtggtgg cgacgggaag ctttggcggt ggcgtgggcc
     4321 agtttggtcg caagcgccag gccgaatcga gccccgaagg cccgatggcg atttcggcgg
     4381 cccccgacgg cagcgtactc atcgtcgatc aggtcaaccg ccgcgtggcg cgttacaaag
     4441 acggcaggtt tgtgtcgcag attccgattg gcggggacac cgtgcaggac ttggcgatcg
     4501 gggccgaggg acgggtggtg ctgctcgatc gtttcgtcga ccgcggcatt ttggtctacg
     4561 gtcccgacgg caagctgata aatgatgtgc cactgccgag caagctcgac ctggggagcc
     4621 tgaccggcgt gtttgctgac gagcatggca tctacgtcga gcgtgagcac ggccaagtga
     4681 cgcaagttgc cgacgcaaac ggcaatcgga tcgagggcag caaagagctt tccgggcggc
     4741 cctctcgcga tggtcagctt gtgctaagcg ctgcgctttt ggaacggcag agcgggcaag
     4801 tgctgattcg cgcccatgac cgcaatcgcg cgacgctggt gtttgaaaaa gtggtgcagc
     4861 tgccgacgcc ggttttgcat ctggtgatgc tcgacagcga tcgcagtggc cggatttatt
     4921 ttgccgccgt taccgcgata gaagaccagg cgccaccgta tgcactgtct gacgaagcgc
     4981 tgttcgcggt gcggctgggt cgcgacggca gcgagcgcgg tcgtttgaag ctgccgccgc
     5041 ttcatggcag tgatgagtca tttcgtcccg tgaccgtcga tgacggtggc aacctgctgg
     5101 tgatgcagtc gagcgaaacc ggtctttcgg tggttcgtta tgcgtttccg tagccgcaaa
     5161 agccgtgtcc tttgcgtcga gagagggcga tgacagccat accgatcgag gattttgcag
     5221 ccgccgccaa gctgccgcct gtcgatggac aagcggtgtt tctgggtcgc atgggctacg
     5281 gggtggcgtg gaaacttcag cagcttgtcg tcgaggcgcg gcaaaacggc cagatccccg
     5341 acaccttgct tttgtgcgaa cacgacgatg tgattacgct cgggagacgg cagaccgggc
     5401 ccgccaatgt gctcttgcgt cgctttccga tctttgaaat cgagcgcggc ggcgatgcga
     5461 cttatcacgg accggggcag ctcgtcgggt atccgatctg ttttctgcgg cccggacccg
     5521 atccggcgca gccgtttggc gaacaggatc tgcacggctt tttgcgcgcg ctcgaggaag
     5581 gtctgctgca cttgctgcgg ctgtgcggcg tcgcctgtga tcgcaagccg aaatacacgg
     5641 gcgtttggac tgctgatcag acgggcaagc tggtgtcttt gggcgtggcg gttcgtcgct
     5701 gggtgacttc acacgggttt gcgctgaacg tgaccaccga tttgtcgcgc tttttggcga
     5761 tcaatccgtg cggcatgtcg gcgcaggtga tgacgtcgct ggctgcgctt ggggttcagt
     5821 gggacggcaa gccagtgtct gtcgatgcgc tgattgcgcc gtgtgcggct tctttgggcg
     5881 cgtcgcttgg tcgtcgtttt gggctgcgca gcgtcgaaga agccgggctc gcggatctgc
     5941 tgcggcaagc gaaacaaaca cccgagctgc cggcgttcag tcctggctaa ccggctcttg
     6001 cagacggcgc agggtcattt catacgcaag ctggagcgct ctttgaccgg gttcaagctt
     6061 gaacggcgac agcttcggca gcgacggttc gtgcagaaga tgcaggtccg gccagtcggg
     6121 cacttcgttt tggacccgga tcactttgcg ccacggcgag tgcgtcggca ggtgatgaaa
     6181 cagcagtcgg gcacccggcg tcgcggtgga aattccgggc cgatcggcaa tgcctccgtc
     6241 gaggtatcga cgatcgccaa ttttgaccgg ctgaaacatg ccaggaaaac agcacgacgc
     6301 gcggatggcc agcgtcaggt cgccttcgtc gagcacttcg gtttgcaggg tatctagcgc
     6361 aaacgcagcc agccgcaccg gaatcgcaca gtcgtcaaaa gtctcgacgg aaacttgcga
     6421 cagtagaccg cgcaggatgt agtcaaacgc ttgtccgcgc aaaagtccgg gaccgctcga
     6481 ttcgtcggca agtcggccaa ttcccagtcg ctgtgcgatg tctcgtagct ttccgcggcg
     6541 cagataaaac ggcagtcccc acagcggatc gacatcccaa aacgccgatc gggaaagtcc
     6601 aagcaaggtg tcgcgcagca cggtcgccgg aagtccagcg ccccacagtc cagcgacgag
     6661 cgcacctgcc gatgaaccgc cgagctgggt gggcgtaagg ccagcttctt ccagcgcgag
     6721 cacgacgccg gtatgggcaa aaaaaccgaa aaatcccgac gacagcacga gttcaaacgg
     6781 ttctgctgcc agatgctcag ccagtgtctt catgagggtt ctcgatgagg ggagggcgca
     6841 atatgaagcg agaaaaatgc ctatttccgc ttgctgcgct tgggcaggtc ggcgagtgcc
     6901 gccagataat cgatcggtgc gtcgctcggt ttgggcacaa gctcggcttc ggtgagcggc
     6961 cccagatcgc gcagtcccga tgccagcgtt cgcaaaagat cgcgcgttcc cggcaaagtt
     7021 ccaagcgccg gcagcagatc ggtttcaccc gcgccatcgg caatcgcacg acccagatca
     7081 tttacggttt caatcgcgat gtggcgacgg cgcaaaaggt cttggagcga gtttcgcagc
     7141 gcttcgatgg tgtagctgtg gcgatcgacc atttcgacgg caacccgcac gacggcggcg
     7201 gcttcgattc gatgatcggc aaacagcgtc agcgcggcct gaaagtagtt gtaaatcgcg
     7261 accagccgcg agccataccg atcaaacttt cccggtggtg agcgacgatc caggtggatg
     7321 aagatccgtt ccaccgcttc ggagtgtggc agcgattcga aaatcgacac cgtgcgcgcg
     7381 gcgtcgtctt catacgtacc ggcggcgcgc aaaatctgtt cgagcaggct gcgatcgatc
     7441 cggcccgcca gcagatcccc atacagcgag tagatgagcg cgtcggactc cgcgtcgtcg
     7501 ccaaagcaca cctcgcgcgt ttcggcggtg acacccgccc gtcccgccag cagcgccggc
     7561 agtttgtagc ccacctgttc gcgcagggcg cgaaatcgcc cggtcagcat gttgcgcagg
     7621 tttggcttga gtacaaactc gtcgaactcg accccgtcga gccgcagctt ttcggtcagc
     7681 actttgcgca tctgccgcgg cgatccggat atgaaacaaa tccgtgctct gccgacggac
     7741 gatttgcgca gctcacgcag tagcgtcgca gcaccgggga cggttcgctt gtcttcggcg
     7801 tcctcgaatg cggtgcggac cagtgacata aaggtgtcaa actcggtgcg caggtaggtc
     7861 ttgtcgagat cccagcggta aatcacgctc gggacggtca gctcggcttg gctgtcaaag
     7921 gcggtcgtct tgtcgttcat cccagcgctc acttgctctc gctctgggct tttaggttgg
     7981 cggcttcgat gagcccgcgc tcgatgtcgt agagcagtcc gctggtgacg ctttttttgg
     8041 cgactttgat cgcgttttga atggccggcg cgtggctgcg accgtgcgct ttgataagca
     8101 agcggtcaaa cccgagcagc ggcgcaccgc cgtactgttt ccagtcggtc agctctttga
     8161 gcttgccgat gccgcccgac agcatcgcca gaccggcgcg ccacatcagc ttttctttgt
     8221 aggcgtagtg ggccaagcgg acgacggttt ccgacacgcc ttcgagcatt tttaatacga
     8281 cgttgccgac aaagccggaa cagaccacaa cgtcagcgac gccgcgaggg atgtcgacgc
     8341 cctcgacgtt gccgataaag tcgacgctgt gcagtgattt gagcttggcg tgggcttcca
     8401 cgacttcttt ggggcctttg cctgcttcgg caccgttcga caacagcgcg actttgggct
     8461 tgggattttt ggaaatccgc gccgcatacg ccgatcccat gatggcaaag ctcaccaagt
     8521 cgtctgccgt cacatcgagc gtcgccccca catccaagat cagcgaaaac ggatcgtctt
     8581 tttcgccgcg ccgcacttcg gtcggataga ccgctgccag tgcgcagcga cgaatccccg
     8641 gcagtctttt ccaatattgc aggcaggaaa gcaccgccgc gccggtattg ccggctgata
     8701 ccagcgccga cgctcgacct tcggcacaaa gccgcgctgc gacggcaatg ctgctgtccg
     8761 gtttgtggag gagcgcatct ttgggcgttt cctccatggt gatgacctgg ctggcgtggt
     8821 ggatcgccaa gcgctccgcg tcgtagcgct gctgcatcag tagctggtgt aggcggggcg
     8881 cgtcaccaac gagaagcagg tgcagcggcg ccccagcgtc cttgtcgggt ttttcgatcg
     8941 tcagggcgca ggcggctttg accacttcct cgggggcgtg gtcacctccc atcgcatcga
     9001 cgacgacggt gatcggttgt ttggcgactc gctcgtgtga atcggtcttg tcgccttttt
     9061 ccgtgactgc gtggctacct gtgtcgcggg cagacatctg gctttccacc atatcatgtg
     9121 cagccgtttg cgccgcttgc ttggcctacc tgtcgcggcg ggatgacttt ggcgcgaaga
     9181 agtcaggccg gcgggcggtt cgaacgtaaa cagaaacggc gggggcatgt cggcaggctg
     9241 tgggggcctg gttccgtctc ggaccggcat aacggttgat ttcatgacca aaaacaccat
     9301 acgatagctc ccaccacaca ccttcgctct gacgaaaagc agacccatac gggcacgttg
     9361 gcggcgaagg gcggctcagc ttgaggcaaa ggggaaggtc agcgtgagcg aggacgacat
     9421 catctactgg caggatgtcc tagagtcggt gcaagcgggg cgaaccgcca acctgaagtg
     9481 tccgttctgt cagcagggcg acattgaggt caaaaagcgc gagcgggtga cgacggtgcg
     9541 ctgtaccgcc aagggctgtg gtcactttat cgaaggccgg ttttcagccg aagatgtggc
     9601 cgaataactg taggttaaat tcatacacaa gctacctaat caggtgatca gcgaagcaag
     9661 aaaacatccg gttggtcgtt ttgctactga attgtaaggg gctggatcgg atatagtctt
     9721 gctctgcggg gattgtagag ttttggggct tacagatagc tgtgtagtgt tttgaatatg
     9781 aagcacacat agcactgttg gtgaggagag aagggaaaca tggcgcgcaa cttcctcgga
     9841 aaaggatggc gctatccggt gatgatcgac cgccacggcg ggatggcgca gagcagtctc
     9901 gacgacttgg tgcgggaatc gatcttgatc atcctcggaa ctgctcccgg cgagcgcgtg
     9961 atgcgtccgt atttcggatg cgacattcac gagctgctgt ttgctcccaa taatgtcaat
    10021 actgcgggcc ttgctgctca ctacgtcgtc gaagcgctcg aaaagtggga acctcgcgtc
    10081 gaagatgtgt ttgccgacgc aaatccagat cccaacgagc cgcacaagct aatcatcgat
    10141 atcaagtaca agattcgcgg ccagaacgtt acgcgaaacc tcgtctatcc gttctatctg
    10201 cgcaggagtg atcaagaatg atcccgacgc cgaaactgga cgatcggacg tatgccgata
    10261 tcatcgccga ggcaattcgg ctgattccgc gatactgccc agagtggacc aaccacaatc
    10321 cctctgatcc aggtatcacc attctcgagc tctgttcgtg gatgacagag cttatcctct
    10381 atcggctcaa caaagttcca gagaagaact acctgacctt cttggacatg ctgggcatca
    10441 agctgcaacc ggcccagccg gcgcgcgcgc tcctgacgtt caagctcgcc gacggcgtgg
    10501 aacgacagac gattcgtcgc ggtctgcaag tgtcgacgcc gcaagccgat gaggaagacg
    10561 cggttctgtt cgagacgcag cgcgatctga cggtgatcag cgccaatctc gatcgctgct
    10621 tttcctattt cgatgaagcg tattcggaca actcgcccta tttgcaaggc gagcgcaccg
    10681 agggctttct gtcgttcgcc ggggccgaac gggtcgatcg cttcatttat ctgggcgacg
    10741 agcggctgat tggcgtaaac gactcgacgg tgctgcgtct gcggatgact gctccggagc
    10801 acggtggtcg cgatctggcc cggcttttga gctgggaata ctggaacggt cgtcgctggc
    10861 gtgagcttac gctgtcccag cttgaagtcg agcgcggcga ggtcatcttc cagggcgtgg
    10921 tcgacatgca aaagaccacc gtcaacgggc tggagaccta ctggctgcgc ggacggctgg
    10981 ccgaagtgcc gcgctcgccg gctgagacgg aagtcgacac ggttcgcgga cggatcgaag
    11041 tcacgggcga aggcatcctg cccgatcagg cgttcgcgaa cctcgacaac aacgtgttta
    11101 tcgcgctgga tctgggcaaa aacgtccatc cgtttggcac cgagcccaag cccgatcacg
    11161 tcctgtattt ggcgtcgcag gaagcgctga gccaagttgg cgtgcagatc gaaatcgact
    11221 ttgcgctgag cgatccgcag attgcgccgc cgccgaagcc gagtgacgac ctgatcctgt
    11281 cgtgggagta cttcgacggc aaaaagtggc gcattttggg caagagcacg ccaagcggca
    11341 agccaccgca gggctgggaa cagagcgtgc tcggctttgt cgacgccacc aatgcgttta
    11401 ccaagagcgg cacggtgcgc tttaaccgtc cgcccgatct gcaacctggc gaagtgaccg
    11461 gcgagcccaa ctactggatt cgcgttcgca tcgaacaggg cgatttcggt gtgccgggcg
    11521 cgtacatgct cgacggggac aagtgggttt ggcgcgacga aagaccgctc aagcccccgg
    11581 cgttcaagag catcggcatt cgctaccaga ccgatctcga tcacctcaag tatttcgttt
    11641 cgtacaacga cttccgctat cgcgatcact cggaagagga aaaaaacgaa taccgtccgt
    11701 tccagccatt ccaggcgatc cccgacgaag gcgcggcgct gtatttgggc tttgatcacc
    11761 gtctgcccaa cgagacgatg agcctgtact tccagtttgt cgagagcacg gtcgtcgagc
    11821 aggactttgc ccacgccgag cacctgacgc agtactacga aaagcgcaag accatctggg
    11881 aacccgagca gcgcatttcg tgggagtact ataacgggcg cgcttgggtg acgcttgtcg
    11941 tcgctgacgg gaccaaaaac ttcaccgagt ccggcttcat cgactttgtc ggtcccgacg
    12001 acatggacaa gacgctgaag tttaccgaag accgcttctg gattcgggcg cggctcgaaa
    12061 tgggtggcta catcaagccg ccgcgggtgc gtcgcatcct gcaaaacacc gtcgaagtcg
    12121 ccaacgtccg caccgtccgg gacgagatcc ttggatcgtc tgacggaacg ccgctgcaaa
    12181 attacacgct tttgcacgga ccactgctcg gcggtcaggt catcgaagtg cgcgaacgcg
    12241 aggagccgct cggcgaagaa gtcgaggaac tgggcgaaca cgcggtgcgg atggccgacg
    12301 atcgcgaagg cggctacatg gtccgctggc gcgaagtcga gagcttcttt gaatcggggc
    12361 ccaagtcgcg gcactacatc attgatccgc tgacgcgaca ggtgcgtttt ggcgacggca
    12421 tccgtggcat ggttccgccg gtcagccgca acaccgtcat cgccgcggaa tatcgcgtcg
    12481 gtggcggcag caaaggcaac gtcaacgctt ttacgctgac gtcgctgacc cgcgcggttc
    12541 cctatattga aaaggtctat aacgtccttc ctgccgtcgg tggtgccgat gcagaaacgg
    12601 tcgagcaggc caaagaacgg gcaccgctgc agatcaagag ccgcgatcgc gccgtcacat
    12661 cggaagactt cgagacgctg gcgctgcgtt cgacgacgag cattgcccgc gccaaggcga
    12721 tgtcatccga cgaccgcgct ggccacgtcc atctggtgat catcccgcgc ggcgacgaaa
    12781 agaacctgga tctgacccgc aagctggtgc cgccgccgga gctgtgccgc tttgtccgca
    12841 actatctcga cgagcgacgg ctgatcacga cgatcctcga cgtgaccaag ccggattatg
    12901 tggagatctc gctgaagatc acgctgatcc gtcgcaccgt cggtcagagc gagcgtctgc
    12961 gcaccgagat cgagcagcgc ctgcgtcgat atctgcatcc gctcgtcggt ggcaaagatg
    13021 gcaagggctg gccgtttggt cgtccggtgt atcgcaccga cctcgtccac atggttgaag
    13081 acatccccgg catcgaagcg attgagtcga tccagatcta cgacgaggac cgtcgcatca
    13141 acgtcgacag cgtgcggctc aaggacgatg aactgccgca cgtcgtcaac gttgccgtga
    13201 ttgagcgcgt ccgtcaggag atgatctgat gctgcacgag cgcgagccga tgcagcgccg
    13261 cctggtccgc gtccccgacg tggttgggct gtccctgcag aaagctctgc tactggttca
    13321 gcacgccggt ctcaaggtcg gggcggtgct gttcaaggaa agctacgagg aaaaggacac
    13381 gatcctcgag cagaagccgc agcgtggcca aatggtctac gcatcggacg acgtgacgct
    13441 gtggatttcg cgcgaaagct acatcaagtg gctgccggcg atttaccaac gctccgacgc
    13501 gacgggccgc aactacgttc gcgacgtgct gtggatcatc cagcacctgt ttggatcgat
    13561 cgagtcggtg ctcgacggaa tccatctgta ctttgactcg tatgaaacgc cggaacagtt
    13621 tctgccgtgg ctggcggcct ggtcggcgat gattctggag cccgactggc cgatcgccaa
    13681 aaagcggcgc ctgatcaaaa aggcgatgga gctttatcga ctgcgcggca cggtgcgcgg
    13741 actgaagctg tttatttcgc tttttaccgg ttttgaaccg catctggcgg aaaacgagtg
    13801 gccgtttaac ggcttccgca tcggggtttc atcgacggtg ggcatcgaca cggtgatcct
    13861 gccgccggtc gacaaagcgc actcgtttgt ctgcgtgatg ccgcacaagt tcaaagatgt
    13921 gtcggcgcag tcgatcattc ggctgcacga gatcatcgag atggaaaagc catctcactc
    13981 gacgtacatg ctcaagtttg aagatccgcg ccaggctgtc gaggagcgcg agttctatcg
    14041 aatcggtgtg tcgtcgggcg tcaacatcgg tcaggaagtg gtcacgccgc tgccgatcag
    14101 cgaggaaacg ggcgagccgg attatccgaa ggaaccggtg atgccgccgc cgccgccaaa
    14161 tccgtcggtg tacgagctgt tcgggaccaa aaagcgacag ctcccggcga tcactggcgc
    14221 tgccaaggcc gacaacgctc ccgtcgaagg tcgcgaaatt acgaccagca agtacggctt
    14281 cgaaggtgcg cgcgagttcc gtctcgacga catcatgaaa gagctgttgg cccgcgccgg
    14341 tctcgaacag agcgtcgatg tatctgtcga ttcgagcgct ggcagtggtg cggcggtcaa
    14401 tccggcggtg gtcgagctgg aaaagaacga catttcagca ggtggcgacg aagcgctgct
    14461 ggaatcgacg ctcgaaagca gcggtccgag tgaaaaagtg ctctattcga cggaccagcc
    14521 caaagcaccg gctgagccag cgacgcacgc tgaaagcgct gcgagtgccg aaggcgaaga
    14581 gagcagcaag actcccgtca ccggagccgc acctgccgcc gatgaggatg gcgacattcc
    14641 gcgcgccgga cttggcgcga cggtcgaggg tggaatccgg ggtcgcaagc ccaagatcga
    14701 catcgatgtg tcggtcgaag aggtcgatgg cgacgcggtc gaggaaatca gcgcagatga
    14761 tctcatctcg gaagtcgaca aaacttcgtc cgagacgagc gacagcaagc acagcgatgt
    14821 gagcccggtg aagacttcgg ccagcgtcga aggaagtgtc aaaaccggct cacctcgcgt
    14881 cgagattgag gtcgaaaagc gcgaagtgat tcgagatgag ggcagcgcgc cgagcggcag
    14941 cgacaagccc gaagacggtt ctaagaaata ggcaacttct aaccagggtt cccagcgagg
    15001 ggcacgatgg cgactgagag tgagagcaaa gtcaagggct tcaagcggat caacttcttc
    15061 aagggcttcc tcaccaccga gcacgactgg aacgatgccg agcggtacca tatcgagaag
    15121 cgccgtctgc acaacaagat gatgcacgcg cccggcgtgg tcttcggctt cagcggcgat
    15181 ctcaaggtca cggcgcgtcc gcgcggcgat ctgtcggtcg aaattcagcc gggctacgcc
    15241 gtcgatggcc agggccgcga tctgatgctg tgggacgccg agataaagac gctggtgctc
    15301 gaagagtaca agctgccgca gaccatctac atcgtcctgc gctactacga acaggaaacc
    15361 gactttatcg cctacaaaga aaacgcggcc tacaaggggc atcggcggat tctggaaggc
    15421 tgcaaggtcg aaatttcgca gacggtgccg gacattcgcg aagaggttga gttggcgcgc
    15481 attctgctcg acaaaaatgc gacgcgaatt cgtgatgccc gcgatccgaa tgagcccaaa
    15541 gcaaacgaaa tcgacctgcg ctttgtgccg cgggccggtg tggcgggcgc gtcgatggac
    15601 ccggtgctca agattcggct ggccctgctt ttggaacggc tgcggcggct gctattggcg
    15661 atggcgcgcg acaacaaagt cgagaccgcg cacgatgcgc tggcgggcgt gattgcggcg
    15721 cagacgctgc atgtgtcgga cctgctcgac aaccgcaatg ggcatgaagt gctcaacatg
    15781 ctggtgcagc tccaagacga gctgagcaag gatgtcgagg tcaaccaccc
//
LOCUS       DAORGN010000117        15752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1532_length_15752_cov_5.468752, whole genome shotgun sequence.
ACCESSION   DAORGN010000117 DAORGN010000000
VERSION     DAORGN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_15752_cov_5.468752"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="thiD"
                     /locus_tag="PKL17_13310"
     CDS             <1..760
                     /gene="thiD"
                     /locus_tag="PKL17_13310"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004959824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNK45760.1"
                     /translation="GAGIAADLKTFSALGVYGMSVVTALTVQNTQGVSAVQSVPAPFV
                     AAQLDALLADSEIAAIKIGMLAEESVTKAVADCLRRQPPPYLVLDTVMVAKGGHKLLQ
                     ESAVDALRSLLVPMASLVTPNLPEAAALLHTEPAQNEQDMVAQGRALIAQGAKAVLLK
                     GGHLLSAKNESPDLFVTAQHEIRFSAPRVPTKNTHGTGCSLSAALAALRPQCKDWPET
                     IARAKDWLSQALQNADRLSVGKGIGPVHHFHRFW"
     gene            851..1843
                     /locus_tag="PKL17_13315"
     CDS             851..1843
                     /locus_tag="PKL17_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45761.1"
                     /translation="MQNRRWLAWGLCAMVGIGIGGTSRGEKSAGSASDSFPVSLEPVL
                     MTTMGKVAGLTGENEAIWKRFEGFVRARHKITELSGPGLSRETAKPAKSAKDQARENG
                     VDVEIVRLRPDVLPGLALFHGVSVDRSQMPIDGPELVRDGGWFALLAGKDHFILPRFT
                     GENPDSTTAVHDDFLICPFGTSLFDMMNCPRLAPRYRAVLRDVEQKGLDPRPVLLQLL
                     ALSVMFDLHLEPLAPAPQTGIAWIRRLDEISGSESPRIPQEVKDRLPAIPTKAPWKLP
                     APLLKKDGSKESASYHAIGYYRHRSQAFMAELKISPDKLYFATTMIASGPHYGP"
     gene            1945..3591
                     /locus_tag="PKL17_13320"
     CDS             1945..3591
                     /locus_tag="PKL17_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45762.1"
                     /translation="MFLEHLLAALRNPHSQLFQLDDELPFDADRYRALLSAETWRRGQ
                     QGELDEATAAWVFRCIADGWVDEHLSDIRDALLELLAVRDADHLDALLCRATEQILGA
                     SPRPQCIADEEAPLLTPADHHLLRTTTQAVTKEQVPRLGARLLARRYAIQLGLLSKQS
                     GTWQRTCLGDAVIELPRIYAWPLLLSLETLQALDPYDRWRVSESALLAMRDRGTFVVR
                     TADFDGDSESGLFPWQRIRRLHRLGLLEPVPQHLSGHETQCEFPFRLTQVAQDAVERV
                     LRTPEHEVMRLAQTLLQERTDDMMQPYRLRQRGIEAVRHSDVIEHAPPSFARQSMVVC
                     DDEAAGLHADSGPLLRMTDLSVSPLRMSEDRAEVPQMDEEREAVPEPTRPGSGGDRGP
                     QEIDLGPLIRRAWGDLQERRVHFSLLGPTTQVFGERRLLVQTLREILRGAAASAQHGS
                     HSLALVTVELAPQDDHVVLFVHDNGIGPPLPALPSLLEMTDDELAEPAETEGDVPSLQ
                     RQQQSLWGIGGELTLLAPRLGGTSLRIILPRNMEDQRSAA"
     gene            3672..5111
                     /locus_tag="PKL17_13325"
     CDS             3672..5111
                     /locus_tag="PKL17_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK45763.1"
                     /translation="MQASRVRSHVAKSRSLALFLLTIGLLGCKPQMVGIGDDDQGLVD
                     AGDPAGPDDLAAPPLPPKIPGCVGDCVVNATCSADKGPTTLSGTVSIPSGLLPVYNAR
                     VFIPTGEEIPGPPQAGAYCDRCDRTPKVFSTTTDINGKFTLRNIPAGRNMPLVIQVGK
                     WRRVLSISEVSECSVNLLDPAETRLPRSRVEGNIPKMALSTGSADALECLLRSRKLGL
                     DDSEFTPESGPGRVNLYAGATGAAQYVSTLNGGARFTASLAQPNPSWWDSYDNWAKYD
                     IVMLSCEGQQYMQYKSQTALQNLEKYVGSGGRVFASHWHNGWMANAQTPQKIQTVATF
                     ANNLNLGTIAALINTNQDKGAALADWLILPSVSGAMPPPRGQLPVMGAKNTVSNLNMS
                     IAQRWVDAPSGASQYFSFDAPIGAAPAELCGQMVFTDLHVSGGVGGDTSQAGTPFPGG
                     CNAATLSAQEKALIFMLFDLTNCLQPPIG"
     gene            complement(5108..5764)
                     /locus_tag="PKL17_13330"
     CDS             complement(5108..5764)
                     /locus_tag="PKL17_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK45764.1"
                     /translation="MSEQPSSDGNTAPDPRMEWAQIVVSHLTDVFAGRCSITLEQLQA
                     LGEESPLLDVLAGIYYLNDELTNRERQRAQAVKDLEATLEQLKAQHAELLASRELTAQ
                     LSTPLMRAGRGVLMLPLLGTLDEERTQLIVSSVLSAIKEERAQTIILDLTGVDSVDVR
                     TAQFLSRMVGAAGLLGTKTVLAGISPQVAQAMVQINIDTRKLITVRDLADALRYVGLT
                     "
     gene            complement(5748..6200)
                     /locus_tag="PKL17_13335"
     CDS             complement(5748..6200)
                     /locus_tag="PKL17_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45765.1"
                     /translation="MMKLPRFLLGPAMPPSIERRVYGPDSSPEEIAAIQARLSRYNAD
                     TFVWEEMPIQSPFSVEVMLREIQRQTANLPSFNLVVNLTQAEPPPQEVRTALYRSLDA
                     LTNMKRCACYTGRNFLLNAAAKFVLVAVRVKVTVHRTLEEALRDVGAA"
     gene            complement(6254..7804)
                     /locus_tag="PKL17_13340"
     CDS             complement(6254..7804)
                     /locus_tag="PKL17_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45766.1"
                     /translation="MKARKILSVRLAVGAILLGGALTVRADSDTTEMLRDIFDLGMRY
                     TSTVEFLGRAVNANVGCEPNCPTPAPDVDTGRRNFGLSADGNSIETSVALFQGASQMI
                     GVGTITSNGRTCSTCHRPDLRDGTGQVVDAIKLGLPHTLPISSVVPTSDPLFTGRPAD
                     DGNHPNGLANLDNHGLVLIRPGRFNPLLSPSDPFRQVIEWRKVPRFVNTALTIGFVND
                     GRMREIVETARGAIFSHTQNFDERFDDLLRVPNPRYPAGPPDFEERPRNIAAFIEATT
                     IDPPELKALLTPTDPIANPTCSNAVGAKCKISDCVRLTGGTQCDLATVLARDPFFTVH
                     ASTDSERRGQAVFAAQCMSCHNTPNVFGNVDHIPGLPLSFPPRYGKAFDVGVAQKNAL
                     GLDFRAFLCADGTTQCPESSRSLKTVVLPLAQVDGTLVQHAVTVDPGTASASGRYEDL
                     YRFKVPQLRRISQLGPYFHDNSAPTLEAVIDHFLSDDYRRSQDGQEHPIVLSPRDKAD
                     LLAFLRIL"
     gene            complement(7820..8836)
                     /locus_tag="PKL17_13345"
     CDS             complement(7820..8836)
                     /locus_tag="PKL17_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45767.1"
                     /translation="MIAPIWTGNDDKGQLLLPLWRGTAAAIEDWVPPPPSGNCEGLKG
                     TVRIDWDKSKNTVHYVVKLRGLPRSPQITRIDGGDPEILDQNLPNHYVAPPAANWWYN
                     PFHPSPKNFPQNPSNGTAYRLWTISTTYGTASTPFYYSGTTLKLQGSAFDYPAGPPPG
                     TFPVSFPIVSIVSSLLFYPNANGNASREWTVPYSQVTGEGGFWSWAPVAFLPHNLCRA
                     VATQPTLGQLRPYAAPFRPANEGLSWKAMMQAGLFFDLTIEEGRPDVPPGGDDNNFTY
                     VYSGLAFLNNAPLMQGGVPWGWHGSLPAIIQNVAPGIQPVPKCGGFVSHPHVTAPLYC
                     QGQH"
     gene            complement(9375..10751)
                     /locus_tag="PKL17_13350"
     CDS             complement(9375..10751)
                     /locus_tag="PKL17_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNK45768.1"
                     /translation="MSVFSRRSLLRAAAASALALPLGGLLPSPRRAYAAPSGGARRVI
                     FFYFPDGVAGPAADGSPSLWHLQGPERGFSLNEQLQGLGTFKDDCVFLNGLSSGPTDT
                     GSHPGGAKKLLTAVDGGNGESIDQYLARTTGSSTLFRHVYLGAMANYNSASGDKHISY
                     PSAGVSATPEDSPMKAFERLFGKTNPSGGGGGGSMPDPIATSVLDTTRKDLEELSARL
                     GSVEKKKLDLHMDALREVERRVKGIPGMTPPPKATCSDPRIDTSGLTDTSLYDPGLFP
                     KILRAQMDLMVQAMACGLTKVGVVQASHHTSELIMSRFPATEMYDPKFDMRSHQASHY
                     GPSQDKSKREFRDYFSQRRWWVQQFAYLLDQLKQRPEADGTMLDYSLVVLCSEICDGN
                     THSHDNLPFIVGGRGGGAVTTGRLLSYGYKRHGDLLVSIARAMKQDIWRFGDGSSGPL
                     SGFLSSGF"
     gene            complement(10762..12996)
                     /locus_tag="PKL17_13355"
     CDS             complement(10762..12996)
                     /locus_tag="PKL17_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNK45769.1"
                     /translation="MKTSAASFSRFVSLPRSAVDAVSVAALVLSLTLTLVGCTEPAVD
                     EDPVASDPDAVAKGAALYVELCSVCHGAKGQGVSAPALLPMKSSASELRQIISERMPP
                     GRPGTCSGNCAGYVAAYVQANMQATNPVCAERSLGARRLRLLTRKEYQNTITDLLPKS
                     GSSCVLPSFSYDPMGRTLRSVHIAGSFNNWPGTVAAGGWPMTYSSTDKRWKAERAIDP
                     GTHSYKFVLDEKDWVTDSSNPRTMPDGFGGQNSVLTVTCKPGAPALDLTTDIPPDARP
                     EGYGFDNNSDVRVVSEQHLEAYRKGAEVAAKQVTDNLAAVLPCDASGGREKVCAEQFL
                     KTFGLRAFRRPLSDSELARYRDLITSQKTFADGISAAVQALLSSPHFLYRSEVGEAQG
                     DGSYKLTNYEAASALSYLFWGSMPDQELFDAAQRGELHDPAKLRAQALRILSSPKSRA
                     VIGSFGLSYLGAEDILSSTKSALLYPDFTDAVRSAMAEETRQLFAYITLDGSGRFDEL
                     FSADYSFLNADLAKLYGAPGVTGSSLGKVSYGSLPRAGVLGHGSVLGSYAHSDQSSPI
                     RRGLFVRRQLLCEELPPPPPNAGGVPQVDPKATTRERFRQHTSVAFCKSCHQYIDDLG
                     FGFERFDAIGKWRETENGQPIDSLGDLNDREALGSLTHAPYNGLRELATMLAESDRAR
                     SCFVRKTFRYARGAQEHVGDDFCSLWKLGRRFRESGGSIRELLLSYVESDALLFRSAE
                     SPSK"
     gene            complement(13069..13782)
                     /locus_tag="PKL17_13360"
     CDS             complement(13069..13782)
                     /locus_tag="PKL17_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45770.1"
                     /translation="MIDVAKSPLAQRQSARSAGETLSGRPPGPGEGRFKILSTGQRLQ
                     KKPEHLTHLRDDETLVEKSHPRMRLRGKLDLLQGLVLDAQRTADSEGASGLVGDLEEV
                     MQLLRSMLGCEVMDTPLPEVKLIGMSPADIRHASHNTHKLYGVPFMYPSIHQGEVVAR
                     LYQCRATAREAELACYEAFPTPIPPDPQNPGERSDLKSALNVLSSALYVMQCKFVGGH
                     YGVRRKPGPLKGWRPAAKN"
     gene            complement(13756..14409)
                     /gene="eutL"
                     /locus_tag="PKL17_13365"
                     /pseudo
     CDS             complement(13756..14409)
                     /gene="eutL"
                     /locus_tag="PKL17_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
     gene            complement(14422..15276)
                     /gene="eutC"
                     /locus_tag="PKL17_13370"
     CDS             complement(14422..15276)
                     /gene="eutC"
                     /locus_tag="PKL17_13370"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HNK45771.1"
                     /translation="MADTLDRGAIEKMVREALRKHGDPRAAVEHVAAATLVSGGEVLL
                     KPSTLQSPAAPAQLRSLVASTPARIAVGRSGTRYTTSTLLKFRADHAAAKEAVMSEVK
                     RETLDRLGFVEVRSQAKDKKHFLQRPDAGRALSEEAKQIISAKLPKGAQLQVIYGDGL
                     SAEAINVNAEPLQAALSHELSARGIRMNPPLFIHLSRVKIMDEVARLIECESAIFICG
                     ERPGLGFADSLSAYYIYRPATGATDADREVISNINPRGFPPAQAAAQIADSIVRILRD
                     KKSGVILG"
     gene            15485..>15752
                     /locus_tag="PKL17_13375"
     CDS             15485..>15752
                     /locus_tag="PKL17_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45772.1"
                     /translation="MLLCSPFFPRLASADPMGQKTATLTVQLDQVQRELMGQETSREQ
                     VQRELALRESDQAKLVEQIEQLKAQPPGPVRDLRLQSLLAQAQDR"
BASE COUNT         3160 a         4660 c         4759 g         3173 t
ORIGIN      
        1 cggtgctggg attgcagcgg accttaagac cttttccgcg ctgggtgtct acggaatgag
       61 tgtggtgacg gcgctgacgg tccaaaacac ccaaggcgtg tcagcggtgc agtccgttcc
      121 ggccccgttt gtcgcagcgc agcttgatgc actgctagcg gacagcgaaa ttgctgcgat
      181 caaaatcggc atgctggcag aggaatcggt gacaaaagcc gtcgccgatt gtctgcggag
      241 acagccaccg ccttacctgg tgcttgatac cgtgatggtg gcaaaggggg ggcacaagct
      301 gctccaggaa tcggcggtgg atgcgctgcg cagcttgctt gtgccgatgg ccagtctggt
      361 cacgcccaac ctgcctgaag cggcggctct gcttcataca gagcctgcgc aaaacgaaca
      421 ggacatggtg gcgcagggac gggcgctcat cgcacaaggg gcgaaggcgg tgcttttgaa
      481 aggcggtcat cttttgtcgg caaaaaacga atcgccggat ctgtttgtca ccgcgcagca
      541 cgaaatccgt ttttctgccc cgcgtgtacc gacgaaaaat acgcatggga ctggctgttc
      601 tttgtcggcg gcgctggcgg ctctgcgtcc acagtgcaaa gactggccag aaaccattgc
      661 ccgcgccaaa gactggcttt cacaggcact gcagaacgcc gatcgcttgt ccgtcggaaa
      721 aggaatcggt ccggtccatc actttcatcg cttttggtga tggcctgccc tgcccgcgca
      781 cgctgcggcg cggactccgt cctttgccaa acagacaccg cgtcacgggc cccatgctat
      841 gatgtttcgg atgcagaacc gtcgctggct tgcttggggg ctttgcgcca tggtgggaat
      901 cggaatcggg ggaacatcac gcggcgaaaa gagcgccgga tcagcaagcg attcctttcc
      961 ggtgtcgctt gaacctgtgc tgatgacgac gatgggaaaa gtcgccggct tgacgggtga
     1021 aaacgaggcg atctggaaac ggtttgaagg ctttgtccgc gctcggcaca aaatcacgga
     1081 gctgagcggc cctggactgt ccagggaaac cgccaagcca gcaaagtccg ccaaagatca
     1141 ggcgcgcgaa aacggcgtcg atgtcgagat cgtgcggctg cgccccgatg tcctgcccgg
     1201 acttgcgctg tttcatggcg taagcgtcga tcgcagccag atgcccattg atggcccgga
     1261 gctggttcgc gatggaggct ggtttgctct tttggcgggc aaagatcact tcatcttgcc
     1321 gcgctttacc ggcgaaaatc ccgacagcac gacagcggtt catgacgact ttctgatctg
     1381 cccgtttggg accagtctgt ttgacatgat gaactgtccg cggctcgcac cgcgttatcg
     1441 ggcggtgctg cgcgatgtgg aacaaaaagg gctcgatccg cggcctgtgc tgttgcagct
     1501 gttggcgctg tccgtgatgt ttgatctgca cctggaaccg ctggcgccgg caccgcagac
     1561 cgggatcgcg tggatccggc gactcgacga gatatcgggc agtgaatcgc cgcggattcc
     1621 gcaggaagtg aaagaccggc tgccagcgat tccaaccaaa gcgccgtgga agctgcctgc
     1681 gccgcttttg aaaaaagacg ggtccaagga atcggccagc tatcacgcga tcgggtatta
     1741 ccgacaccgc agccaagcct tcatggccga gctgaaaatt tcgccggaca agctgtattt
     1801 cgccaccacg atgattgcgt cgggaccgca ctatgggccg taaacggaaa gcgccacgcc
     1861 cgacgggatg atgactgcgg tcaaagcgcg ttttgacccc atgtcctact tggcgcactc
     1921 aaccgcacaa tgtatggtgc gcccatgttt ttggagcacc tgcttgccgc gctgcgcaat
     1981 ccgcacagcc agctatttca gctcgacgac gaactgccgt ttgatgccga tcgctatcgg
     2041 gcgcttttgt cggcagaaac gtggcgacga ggacagcagg gcgaactaga cgaagcgacc
     2101 gcggcctggg tgtttcgctg cattgccgac ggctgggtcg acgaacatct gtcggacatt
     2161 cgcgatgcgc tgctggagct tttggcggtg cgcgatgccg atcacttgga tgcgctgctc
     2221 tgccgggcga cggaacaaat cctgggcgcg agtccacgtc cgcagtgcat tgccgatgaa
     2281 gaagcgccgc ttttgacgcc agctgatcac cacctgctgc gaacgacgac ccaggctgtg
     2341 accaaagaac aggtgcctcg actcggcgcg cggcttttgg ccaggcgcta tgcgatccag
     2401 cttgggcttt tgtcaaagca gtcggggacg tggcaaagaa cgtgtctggg cgacgcggtg
     2461 atcgagctgc cgcgaatcta tgcctggccg ctgctgctgt cgctggaaac gctgcaagcg
     2521 ctggacccat atgaccgctg gcgggtgtcg gaatcggcgc ttttggcgat gcgggatcgt
     2581 ggaacatttg tcgtgcgaac ggcggatttc gacggggatt ctgaatccgg gctgtttccc
     2641 tggcagcgca ttcggcggct gcatcgtctg gggcttctgg aaccggtgcc gcaacacttg
     2701 tcggggcatg aaacccagtg cgagtttccg ttccgcttga cccaggtggc gcaggatgcc
     2761 gtcgagcggg tgctgcgaac gccagaacat gaagtcatgc gtctggccca aacgctcctg
     2821 caagagcgca ccgatgacat gatgcagccc taccggctcc gccagcgcgg catcgaagcc
     2881 gtgcgtcatt cggatgtgat cgaacacgcg ccgccttcct ttgcgcgaca gtcgatggtc
     2941 gtttgtgacg acgaagcggc tgggctgcac gccgattcgg gccctcttct gcgcatgacg
     3001 gacctttcgg tgtcgccgct gcggatgagc gaagatcgtg cggaagtgcc ccaaatggac
     3061 gaagagcggg aagcggttcc agagcccacc cgcccaggtt ccggggggga caggggtcca
     3121 caagagatcg atctggggcc acttatccgt cgtgcgtggg gtgatctgca agaacgtcgg
     3181 gttcattttt cgctgctggg accgacgacg caggtgtttg gtgaacggcg gcttttggtg
     3241 cagacgctgc gcgagatcct gcgcggtgcc gcggcgagtg cccagcatgg ctcgcacagc
     3301 ttggcgctgg tgacggtcga gctggccccg caggatgatc atgtcgtgct gtttgtgcac
     3361 gacaacggaa ttggcccacc actgccggcg ctgccgagcc ttctcgaaat gaccgatgac
     3421 gaactggcag aacccgccga aacggaaggc gacgtaccca gtcttcagcg tcagcagcag
     3481 tcgctgtggg gaatcggtgg tgagctgaca ctgcttgcac cgcgccttgg cggaaccagt
     3541 ctgcgcatca tcctgccgcg caacatggaa gaccagagat ccgccgcgta gccgacgcag
     3601 acgataactt cccgaaaagc ccacacagac cgccgcaacc acggcgcggc gtaggccaag
     3661 cgaggtcgcc gatgcaagca agtcgagtcc ggtcgcacgt agcgaagtca cggtccctgg
     3721 ctctattcct tttgacgatc ggtctgcttg gctgcaagcc gcagatggtc ggaatcgggg
     3781 acgatgacca agggctggtc gatgcaggtg accccgcagg ccccgatgac ttggctgcac
     3841 cgccacttcc gccgaagatt cctggctgtg ttggtgactg cgtggtcaat gcaacgtgca
     3901 gcgcggacaa aggccccacg acgctgagcg gtacggtgtc gattccgtcg gggcttctgc
     3961 cggtctataa cgcgcgggtc ttcattccaa ccggcgaaga gattcctggt ccgccgcagg
     4021 ccggcgcgta ctgcgaccgc tgtgatcgga cgccgaaggt gttttcgacg acgaccgaca
     4081 taaatggcaa gttcacgctg cgcaacattc cggcgggacg caacatgccg ctggtcatcc
     4141 aagtcggaaa atggcgacgg gtgctgtcga tttcggaagt gtcggaatgt tcggtcaatc
     4201 tgctcgatcc ggcggaaacg cggctgccgc gcagtcgggt tgaaggcaac attccgaaga
     4261 tggcgctgtc gacgggaagc gccgatgcgc tggagtgtct tttgcgcagt cggaagctgg
     4321 ggctggatga ctccgagttc acgccggaat caggtccggg tcgcgtcaat ctgtatgcgg
     4381 gggcaaccgg agccgcgcag tatgtgtcga cgctcaatgg cggggcacgg tttactgcgt
     4441 cgctggccca gcccaatcca agctggtggg acagctatga caactgggcg aagtacgaca
     4501 tcgtgatgct gtcgtgcgaa ggccagcagt acatgcagta caaaagccaa acagcattgc
     4561 aaaacctgga aaaatacgtc ggcagcgggg gccgggtgtt tgcatcgcac tggcacaacg
     4621 gctggatggc gaatgcgcag acgccgcaga agattcagac cgtcgcgacg tttgcgaaca
     4681 acctgaactt gggaacgatt gcggcgctta tcaacacaaa ccaggacaaa ggcgcggcgc
     4741 tggcggactg gctgatattg ccgtcggtgt cgggggcgat gccgccgccg cgggggcagc
     4801 tcccggtgat gggggcaaaa aatacggtgt cgaacctgaa catgtcgatt gcgcagcgct
     4861 gggtcgatgc gccgagcggt gcgtcgcagt acttttcgtt cgatgcaccg attggcgcgg
     4921 cccctgcgga gctctgcggt cagatggtgt ttaccgatct gcacgtcagc ggcggagtgg
     4981 gtggcgatac atcccaagcg ggcacgccat tcccaggcgg atgcaacgca gcgacgctgt
     5041 cggcgcaaga aaaggcgctg attttcatgc tgttcgatct gacaaactgc ttgcagccgc
     5101 cgattggcta ggtaagaccc acgtaacgca gcgcatcggc gagatcgcgc accgtgatga
     5161 gcttgcgggt gtcgatgttg atttgaacca tcgcttgggc gacctgggga ctgatgccgg
     5221 cgagcacggt tttcgtcccc agaagtcccg cggcaccgac catccgcgac agaaactgcg
     5281 cggtgcgaac atcgacgctg tcgacgccgg tcaggtccaa aataatggtc tgggcgcgtt
     5341 cttctttgat ggcagaaagc acgctggaca caatcagctg cgtgcgttct tcatccagcg
     5401 ttccgagcag aggcagcatc agcacgcccc gtcccgctcg catcagtggc gtcgaaagct
     5461 gcgcggtcag ttcccgcgat gccagaagtt ccgcgtgctg cgctttcagc tgttcgagcg
     5521 tggcttccag atccttgacc gcttgcgcgc gctgtctttc gcggttggtc agctcgtcgt
     5581 tgagataata aattcccgcc agcacatcca gaagcggcga ttcttcgccg agcgcctgaa
     5641 gttgttcgag cgtgatcgag cagcggcccg caaacacatc ggtcaaatgg gacacgacaa
     5701 tctgcgccca ctccatgcgc ggatcggggg ctgtgttgcc gtctgaacta ggctgctccg
     5761 acatcgcgca gcgcctcctc gagcgtcctg tgcacggtca ctttgacgcg caccgccacg
     5821 agcacaaact tggccgccgc attgagcaga aagttgcgcc cggtgtagca cgcacagcgc
     5881 ttcatgttgg tcagcgcgtc gagcgaccga tacagcgccg tgcgaacttc ctgcggcggt
     5941 ggttcggctt gggtcaagtt caccaccaaa ttgaacgatg gcagattcgc cgtctgtcgc
     6001 tggatttcgc ggagcatgac ttcgacggaa aatggcgact gaatcggcat ctcttcccag
     6061 acgaaggtat cggcattgta ccggctcagt cgggcctgga ttgctgcgat ttcttcggga
     6121 gagctatccg gaccgtagac tcttcgctcg atggatggtg gcatggctgg cccaagtagg
     6181 aaacgcggaa gcttcatcat accaaacccc atgccttcgg aacaggaccg gctgggcggg
     6241 ccggcttttc tgactacaag atccgcagga atgccaacag gtcggccttg tcacgcggcg
     6301 acagcacaat cgggtgctct tggccatctt gggatctgcg gtagtcatcg cttaggaagt
     6361 ggtcgatgac ggcttccagc gtcggagcag agttgtcgtg gaaatacggc ccaagctggc
     6421 tgatgcgacg gagctgcggc accttgaagc gatacaggtc ttcataccga cccgacgcag
     6481 acgccgtacc tggatcgacc gtgacggcat gctgcacaag cgttccatcg acctgcgcca
     6541 gtggcaacac cacggtcttt aggctgcgcg acgactcggg acactgtgtc gttccgtcgg
     6601 cacacaggaa cgcccgaaaa tccagaccga gcgcattttt ctgcgcgaca ccgacgtcaa
     6661 aggccttgcc atagcgcggc ggaaagctga gcggcagacc cggaatgtga tcgacgttgc
     6721 caaagacgtt cggcgtgttg tggcagctca tgcactgcgc ggcgaacacg gcttggccac
     6781 gccgctcgga atccgtcgaa gcatgcaccg taaaaaatgg gtcacgcgcc agcaccgtcg
     6841 ccaaatcaca ctgcgttcca cccgtaagcc gcacgcagtc ggaaattttg cacttggcgc
     6901 ccaccgcatt ggaacaggtg ggattcgcga tcgggtccgt cggggtcagc agcgccttga
     6961 gctcgggcgg atcgatggtc gtcgcttcga taaacgcggc gatgttgcgc ggacgttcct
     7021 cgaagtccgg tgggcccgcc gggtagcgcg ggtttggtac acgcagcaga tcatcgaagc
     7081 gctcgtcgaa gttttgcgta tgcgaaaaga tcgcgccgcg tgcggtttcg acgatttcac
     7141 gcatccgacc gtcgtttaca aaaccaatcg taagcgcagt gttcacaaag cgcggcacct
     7201 tgcgccactc gatgacctga cggaatggat cgctgggcga caaaagcgga ttgaagcgcc
     7261 ccggtcggat cagcaccagt ccgtggttgt cgaggttggc caagccattt gggtgattgc
     7321 catcgtcggc gggtcggccc gtaaacaacg gatcactggt tggcaccacc gacgaaatcg
     7381 gcagcgtgtg cggcagaccg agctttatcg catcgacgac ctggccagtt ccatcgcgca
     7441 gatccggacg gtgacacgtc gaacaggtcc gtccgttgct ggtgatggtg ccgacgccga
     7501 tcatctgcga tgcaccttga aacaatgcga cgctggtttc gatcgagttt ccgtcggcgc
     7561 tcaggccaaa gttgcggcgc ccggtatcga catcgggtgc cggtgttggg cagttgggtt
     7621 cacaacctac gttggcgttc accgctcgtc ccagaaattc gacggtgctg gtgtagcgca
     7681 tgcccaagtc aaagatgtcg cgcagcattt cggtcgtatc ggaatcggcg cgaaccgtca
     7741 gcgcaccacc gaggaggatc gcccccactg cgagccgaac ggacaaaatc ttgcgtgcct
     7801 tcatcggagt cctcctttcc tagtgctgcc cctggcagta aagcggtgcc gtgacgtggg
     7861 gatggctgac gaagcctccg cacttgggaa ccggctggat gccgggcgcg acattctgga
     7921 tgatggctgg cagcgagccg tgccatcccc acggaacccc accttgcatc agcggtgcgt
     7981 tgttcaggaa cgccagcccg ctatacacat aggtgaagtt gttgtcgtct ccgccgggcg
     8041 gcacatcggg acggccttcc tcgatggtca ggtcgaaaaa cagaccggcc tgcatcatcg
     8101 ctttccacga caaaccttcg tttgctggac ggaacggtgc tgcatacggt cgaagctgcc
     8161 cgagcgtcgg ctgcgtcgcc accgcacgac agaggttgtg cggcaaaaag gccaccggcg
     8221 cccacgacca gaaaccgccc tcgcccgtca cttggctata cggcacggtc cattcccgtg
     8281 aagcgttgcc gtttgcgttc ggataaaaca gcagcgagct gacaatcgac acaatcggga
     8341 acgacaccgg gaatgtgccc ggcggtggcc cggcggggta gtcaaacgca cttccctgca
     8401 acttgagcgt tgttcccgaa tagtaaaatg gtgtcgatgc cgttccgtat gtcgtcgaaa
     8461 tcgtccaaag acgatatgcg gtcccatttg atgggttttg cggaaagttc ttcggcgacg
     8521 gatggaatgg attataccac cagtttgctg ccggcggcgc aacgtaatga tttggtagat
     8581 tctgatccaa gatttctgga tctccaccat caatgcgcgt aatttgcgga ctgcgtggta
     8641 gaccacgtaa ttttacaaca taatggaccg tgtttttcga cttgtcccag tcgatgcgca
     8701 ccgttccttt cagcccttcg cagtttccac tcgggggcgg aggaacccag tcctcgatgg
     8761 cggctgctgt tccacgccac agcggcaaca gcagctgccc cttgtcatca tttcccgtcc
     8821 aaatgggagc aatcaccttg cagtccgctg gcgtccaatt cagattgttt ggcgggctgt
     8881 ttagatatgc atttacgatg ggaagatcgc tcggcgattt gttgcttcca tcgcattttg
     8941 tcaatgcgct gccttgttct acatcatcga gtgactgatt cgtagcgcct tgacaactga
     9001 ccaacaaaat acccattgca aaaacgaagg tccgggacat ccgcaatacc tccaagtagg
     9061 gctgacaaat aaaagtctag ttggccggac ctgctaaaca aggcaatctt ttgattctat
     9121 tacaaacaaa aaaatgcaat catttcccat gtttccatgg tgatctgttt cacaatagaa
     9181 gacttacgct ccttttgtga tctgcgtcac tgcccaatgt gcgctatatc acgtcgtgat
     9241 tttcccccgc tgtggagtca gtgggatcga cgtcagcgcc gcgaatacac ccatccgatc
     9301 gcggtgccag gtgtccagaa gccacgcaca gcgacggcaa acaaacaggt gcaatcagcc
     9361 gcaagcccag gtcatcaaaa accggacgac aaaaatcccg aaagaggccc gctgcttccg
     9421 tcgccaaaac gccagatgtc ctgcttcatc gcccgcgcga tggataccag cagatcgcca
     9481 tggcgcttgt atccatagga aagcagtcgc cccgtcgtga ccgccccgcc gccgcgccca
     9541 ccgacgataa atggcaggtt gtcatggctg tgggtgttgc cgtcgcagat ttcggaacac
     9601 agcaccacca gcgaatagtc cagcatcgtg ccgtccgctt cgggccgctg cttgagctga
     9661 tcgagcagat acgcaaactg ctgcacccac cagcgtcgct gcgaaaagta gtcgcggaac
     9721 tcgcgcttgg acttgtcttg gcttgggccg tagtgcgacg cctggtggct gcgcatgtcg
     9781 aacttcggat cgtacatttc cgtcgctgga aagcggctca tgatgagctc acttgtgtga
     9841 tgcgacgctt gtacgacgcc gactttggtc aaaccgcacg ccatcgcctg caccatcaga
     9901 tccatctggg cgcgcaggat tttgggaaac agccccggat cgtacaggct tgtatcggtc
     9961 agtccgctcg tatcaatccg cggatcggaa caggtcgctt tgggcggcgg cgtcattccg
    10021 gggatgccct tgacccggcg ctcgacttcg cgcagcgcat ccatgtgcag atcgagcttc
    10081 tttttttcga cgctgccaag cctggcggaa agctcttcca ggtctttgcg ggttgtatcg
    10141 agcacactgg ttgcgatcgg atcgggcatg ctgccgccac cgccaccgcc cgacggattg
    10201 gtcttgccaa acagccgctc gaacgccttc atcgggctgt cttcgggcgt cgcgctgacc
    10261 ccggcgcttg gatacgagat gtgtttgtcg cccgatgcac tgttgtagtt ggccatcgcg
    10321 cccaggtaga cgtgccgaaa cagcgtcgac gatcccgttg tgcgcgccag atactggtcg
    10381 atcgattcac catttccacc atcgacagcg gtcagcagtt ttttggcgcc gcctggatga
    10441 ctgccggtat cggttggtcc cgaagacaga ccgtttagaa acacgcagtc atctttgaac
    10501 gtcccaagcc cttggagctg ttcgtttaag gaaaagccgc gctcggggcc ttgcaagtgc
    10561 cacagactcg gactcccatc ggcggctgga cccgctacac catcgggaaa atagaaaaag
    10621 atgacccgcc gcgcgccccc cgatggggcc gcataggcgc gacgcggaga cggcagaagt
    10681 ccgcccagtg gcagcgccag tgcagatgcc gcagcagcgc gaagcagtga tcgtcgtgaa
    10741 aatacgctca tcagatgcct cttattttga cgggctctct gcactccgga acagcagtgc
    10801 atcgctttcg acataggaca ggagcagctc gcggatgctg ccgccacttt cacggaatcg
    10861 ccggcccagt ttccacagtg aacagaagtc gtcaccgacg tgttcttggg ccccgcgcgc
    10921 ataacggaat gtcttgcgaa cgaaacagct ccgcgcgcga tcggattccg caagcatcgt
    10981 ggcaagctcg cgcagaccgt tatagggggc atgggtcaag gaacccagcg cttcgcgatc
    11041 gtttaggtct cccaagctgt cgatcggctg tccgttttct gtttcgcgcc atttcccaat
    11101 cgcatcgaag cgttcaaaac cgaagcccag atcgtcgatg tactgatggc agcttttgca
    11161 aaacgcgacg cttgtgtgct gacggaatcg ctcgcgcgtg gtggctttcg gatcgacttg
    11221 gggaacgcct cctgcattcg gtggcggcgg tggcagttct tcacagagaa gctgccggcg
    11281 cacaaacaga ccgcgccgaa tcggcgagct ttgatccgag tgcgcatacg atccgagcac
    11341 acttccgtga cccagcactc ctgcgcgggg cagcgaacca taggacactt tgccaaggct
    11401 gcttcccgtc actccggggg cgccgtacag cttggccagg tcagcgttta ggaaggaata
    11461 gtcggcggaa aacagctcat caaaacgacc actcccatcc agtgtgatgt aggcgaaaag
    11521 ttggcgtgtt tcttctgcca tcgcactgcg gactgcgtcg gtaaaatcgg gataaaggag
    11581 tgcgctcttg gtgctcgaca gaatgtcttc cgcaccaaga taagacagac caaacgaacc
    11641 gatcaccgcg cgacttttgg gactgctaag gatccgcagt gcctgcgcgc gcagcttggc
    11701 tggatcgtgc agttctccgc gctgcgcagc atcgaacaat tcctgatccg gcatgctgcc
    11761 ccaaaacaga taggaaagcg cgctcgcggc ttcatagttg gtcagcttgt acgatccatc
    11821 gccctgcgct tcgccgactt ccgatcggta cagaaaatgt ggactgctga gcagcgcttg
    11881 cactgctgca ctgattccat ctgcaaaggt cttctgggac gtgatcagat cgcgatagcg
    11941 tgcaagttcg gaatcgctca gcggtcgtcg gaatgcgcgc agtccgaacg tctttaggaa
    12001 ctgctctgca cagacttttt cgcgaccccc actcgcatca catggcaaca ccgcggcgag
    12061 gttgtctgtg acttgtttgg ccgcaacttc cgcacctttt cgataggctt ccagatgttg
    12121 ttctgacacc acgcgcacat cggaattgtt gtcaaaacca tatccttccg gtcgtgcatc
    12181 gggaggaatg tccgtcgtca aatcaagcgc tggtgcgccc ggtttgcacg tcaccgtgag
    12241 tacggaattc tgtcctccga acccatctgg catcgtgcgc ggattgcttg aatctgtgac
    12301 ccagtctttt tcatccagta caaacttgta ggaatgcgtt ccggggtcga tcgcacgctc
    12361 tgctttccag cgcttgtccg tcgaggaata cgtcatcggc cagccccccg ccgcgaccgt
    12421 tccgggccag ttgttaaagc ttccagcgat gtgaaccgat cgaagcgtgc gccccattgg
    12481 gtcataggaa aacgacggca gaacgcaaga ggaacccgac ttcggcagca gatccgtgat
    12541 cgtgttctgg tattccttgc gggtcagaag tcgcagccgc cgcgctccca agctgcgctc
    12601 ggcacagacg ggatttgtcg cttgcatgtt cgcttgcaca tacgcggcga cgtatcctgc
    12661 gcagtttccg ctgcatgtcc ctggacgccc cggtggcatg cgttcgctga tgatctgccg
    12721 tagttcactt gccgacgatt tcatcggcaa tagcgcgggg gccgaaacac cctgcccttt
    12781 tgcaccgtga cagaccgaac acagctcgac atacagcgcc gcgcctttgg caaccgcatc
    12841 gggatcgctg gcgaccggat cttcgtcgac agccggttcg gtacagccca caagtgtcag
    12901 cgtgagtgaa agaacaagcg ccgccacaga aacagcgtcc actgcgctgc gcggcagact
    12961 gacaaaacgc gaaaacgaag cagcagaggt cttcacatcc cggagcctac aacaattccg
    13021 gaaagacaag ttcccaatcc gtgcgcgcgt tttttccgtc gggatggatc agtttttggc
    13081 ggcggggcgc cagcctttta gggggccggg tttgcgacgg actccgtagt ggccgccgac
    13141 aaatttgcac tgcatcacat acagcgcgga cgataaaaca ttgagcgcgg acttcagatc
    13201 cgatcgctcg cccggattct gtggatcagg cggaatgggg gtaggaaacg cttcgtaaca
    13261 ggccagctcg gcttcgcgcg ccgtcgcccg acactggtac agccgcgcca ccacttcccc
    13321 ttgatgaatc gacggataca taaaggggac gccgtacagc ttgtgggtgt tgtgggaagc
    13381 gtggcgaatg tctgccggac tcatcccaat cagtttcact tcgggcagcg gcgtatccat
    13441 cacctcgcag ccgagcatcg atcgcaaaag ctgcatcact tcttccaaat caccgacgag
    13501 ccccgatgcg ccttcggaat cggcagtccg ctgcgcatcg agcaccagac cctgaagcag
    13561 atcgagcttg ccgcgcagtc gcatccgcgg atggcttttt tccaccagcg tttcgtcgtc
    13621 gcgcagatgg gtcaggtgtt ccggtttctt ttgcagccgc tgtccggtcg aaaggatttt
    13681 gaagcgacct tcgcccggtc ccggtggacg gcctgacaac gtctcgcccg cgctgcgtgc
    13741 ggattgacgc tgggcgaggg gtgacttggc cacgtcaatc accgcggcgg caaacgcttc
    13801 cgctgcggct tcacaggcgg gcaaagaccc cgaaagatac gcgcctgcga agttcgtttc
    13861 cgtcggtggg ccaaaaaact tggtcagttc cacgcccccg actttgatcg cggcatcgac
    13921 ggcaatcatc gcttccagcg ggggtgcaat cagatacgcg agcggctctc cgacggatac
    13981 accagccagc ttggacagat atcgtcccgt cgcacgcacc acatgtggaa agaaacagag
    14041 ccgcccatgg ccatcggcgg aataaaacca cgcttcgtcg cgcagacagc gctgacaggc
    14101 ttccagtgcg tggtgcactt cgtcgatgtc gtggctcgcg taacagccaa tcacttcacc
    14161 ggacagcgga cctgatgcgt gcgctgcccc ggcgtaaaac gacttggcgt ataccacgtc
    14221 agcgtcggcg gctttggttc cttcatcgag cgcgacatac agcgcatcgt cgctcgtgca
    14281 ggtaataaat cccaccgcgc gaatcccatc tggcagacca agctgctgcg cgagaggatc
    14341 tgccacctgc gcaatggtcc gcaccgacag gacttgtggc tttacgggca aaagagaaaa
    14401 acgcgccatc gtctttgccc tttatccgag gattacaccc gactttttgt cgcgcaaaat
    14461 gcggacgatg ctatcggcga tctgcgcggc ggcctgtgcc ggaggaaagc cccgcgggtt
    14521 gatgttcgaa atcacctcgc gatcagcatc ggttgctccc gtcgctggcc gatagatgta
    14581 ataggcagaa aggctgtctg cgaaaccgag tcctggtcgc tccccgcaga tgaaaatcgc
    14641 tgattcacac tcgatgagcc gcgcaacttc atccatgatt tttacgcgcg acaagtggat
    14701 aaaaagcggc ggattcatcc gaatgccgcg tgccgacagc tcatgcgaca gcgcggcttg
    14761 cagtggttcg gcattcacat tgatcgcctc ggcggacagt ccgtcgccgt agatcacctg
    14821 aagctgcgcg cctttgggca gcttggcgct gatgatctgc ttggcttctt ccgacagcgc
    14881 gcgtccggcg tcgggccgtt ggagaaagtg ctttttgtcc ttggcctggc tgcgcacctc
    14941 gacaaaaccc agccggtcga gcgtctcgcg cttgacttcg gacatcaccg cttctttggc
    15001 ggcagcatgg tcggcgcgaa acttcagcag cgtcgacgtg gtatagcgcg ttcccgatcg
    15061 cccgaccgca atccgcgccg gcgtcgacgc gacaagcgag cgaagctggg ccggggctgc
    15121 cggactttgc agcgtacttg gctttagcag gacttcgccg cccgaaacca gcgtcgctgc
    15181 ggcaacgtgc tcgactgccg cccgtgggtc gccatgcttg cgcagcgcct cgcggaccat
    15241 tttttcaatc gcgcctcggt cgagtgtgtc agccatgtgc ggcggtttat aacacgcatc
    15301 aaaaaactga ggcgagcagt tcccgccgca gaatgtccgc gacacaaaag aagagcgagc
    15361 atggcgggca cattgtttgc aaaactgtgg caataggttc tgtttctcga tgaaggcgaa
    15421 atcttccagg cacgctcgct gcatcccagg ttggctcggt ctcagacggc tgaccttggc
    15481 tgcgatgctg ctttgttcgc cgttttttcc tcgccttgcg tcggcggacc cgatgggtca
    15541 aaaaaccgcg acgctgaccg tgcagcttga tcaggtccag cgcgagctga tggggcagga
    15601 aacttcccga gaacaagtcc agcgcgagct tgctctgcgt gaaagtgatc aagccaagct
    15661 cgtcgagcag attgagcagc tgaaagccca accgccgggg cccgtgcggg atcttcggct
    15721 tcagtcgctg ctcgcccagg cccaagatcg cg
//
LOCUS       DAORGN010000118        15601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1552_length_15601_cov_5.361765, whole genome shotgun sequence.
ACCESSION   DAORGN010000118 DAORGN010000000
VERSION     DAORGN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1552_length_15601_cov_5.361765"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKL17_13380"
     CDS             <1..584
                     /locus_tag="PKL17_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK45773.1"
                     /translation="QPNVSAGSVSQFLSTGDINGDGNVDFVSNDFSSGAINLRFGNGT
                     GGFSPAQTIATGLQATAARFADVNQDGALDIVHLLYGTSKGLVFLGNGTGSYANPTQF
                     TAANNPYNVVVNDLNRDGVPDLVVSNDGSSNMTVLLGDGKGGFASNATLATGANPYFL
                     LTADLNKDGNMDIVSSDRGSSVLSVYLAVDQCK"
     gene            complement(684..1949)
                     /locus_tag="PKL17_13385"
     CDS             complement(684..1949)
                     /locus_tag="PKL17_13385"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK45774.1"
                     /translation="MVSTPVSPYTLSFLDARIERRYLDEREKRRSVGQTLISYGLAIT
                     MWLLIVLWSFIMPAHGGATLRNIVFVTALPLLVGAMLLCIPTATRSFAFRIGPFVDFL
                     IGLSIPIAQSYLPGRFDDYSLFVLTIYCVGVHAMMGLRFLTASAISVALFGIYIGVIA
                     LKTPLPQPFVISAALWCFAVQLLAMRISYLDERSRKRDFFRNQLLDDARHKSDTLLLN
                     ILPSSIAQRLKSEPGAIADAFDQVTVLFADLAGFTSLSARLSPADVLALLNRVFSAFD
                     EIADRHGLEKIKTIGDAYMVAGGLPAANKDHACAVAEMALEMRRVIADIHDYGGLAMR
                     IGIHTGPVVAGVIGKKKYSYDLWGDTVNTASRLESHGKPGAIQISDETRAALGERYLF
                     ETRGVIELKGKGPVRTHWLLGRADGMADG"
     gene            2159..3403
                     /locus_tag="PKL17_13390"
     CDS             2159..3403
                     /locus_tag="PKL17_13390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNK45775.1"
                     /translation="MKIGFVPASQPEILSKLPPLGPRVRRIIIHAWDGFWDDRCSLAA
                     SSLSYQTALALVPLLAIALALLKYGGQLEDGSRLLSVVVHQVFPSSQEAQAEVVNRLS
                     TFSGNFTAGGLGSFGIVAALAVGFFLFLSVESIWNHIWESHRERTYVERFLLFYTGIT
                     LIPFVVTLSFVQTEKLWGSFAVVLPYLSPLLTTVMLTLANRLIPTLRVRWRAALAGGA
                     TAAVLLEISKFALGRYLTWVATSYKSIYGALGVLPLFLLSIYLWWLIVLWGVEVARSV
                     QRLPWQLRIHDDANPHDAQLTINGPLAVRLLTDVARHFKNGQKSLPVDEITQRHGLPE
                     SIVRKILNRLAKTGFVVEHEDGYLLARPPDGIRVEEVLRLFQPTQYLHADVEPDQLDQ
                     VLAKLEKQQQQVLADTTLATLI"
     gene            complement(3400..3762)
                     /locus_tag="PKL17_13395"
     CDS             complement(3400..3762)
                     /locus_tag="PKL17_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45776.1"
                     /translation="MYLRAVCLSLVCSLGFGCAHPGPPLSEPAKITDSTRAVSCGKGT
                     ELSRGTSFQTYTAAWCQRPDGTRHGEYVDWWENGMKKSAGMYREGRRDGVWTFFRESG
                     EVDSRLEYRDGAQPPPAP"
     gene            3875..4420
                     /locus_tag="PKL17_13400"
     CDS             3875..4420
                     /locus_tag="PKL17_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45777.1"
                     /translation="MRGRLLLVLCLLLLCAMPSLGWAFDPAFAVPVLRPSAGASWIVS
                     GEREDGFALHVPVGALVGWAPNGLARLFDRTPFLLPELGYELRVTKNHRSDAFSAGLG
                     VGYGVLTLAIVSYTPRFTVGSSDGQTALGFRHSVAGHFLLSVIYLELSHEVLRAGSIT
                     SHDIQLSAGLNVGTLLIPLFF"
     gene            4550..4972
                     /locus_tag="PKL17_13405"
     CDS             4550..4972
                     /locus_tag="PKL17_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45778.1"
                     /translation="MSDSSYADALKKRLWRRLIVATMAAAALFAHSCSDITTSTLLCE
                     DAASRIFECCSAHPAVSCHENGCADQRPNFSQATAKCIRSSSCDALRQAGICDVTDWH
                     MPSYASCIDGQAICQNDGGVCPASVAPACAALGRLTCS"
     gene            4963..5628
                     /locus_tag="PKL17_13410"
     CDS             4963..5628
                     /locus_tag="PKL17_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45779.1"
                     /translation="MFLKSILVAALLCGATPSALAQITPDAPQAPQLGQPLTEAPPSL
                     PPPAVVSSEPGQARFHKPAFTMAVVGGASFQYIIETPFLSGGAELRLGFMTRKVEFTT
                     RLRMLAGSTLAGLVLLAPSISAGVMVPISQRVRIGAEVTLPPFVRGMLIRYVTQSDMG
                     RAALVGAGFETSVDIVQWKDNRALFWVGTLGVDVTAVRVNGDSGQTASVGPNLFTGIG
                     YRI"
     gene            5655..6671
                     /locus_tag="PKL17_13415"
     CDS             5655..6671
                     /locus_tag="PKL17_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNK45780.1"
                     /translation="MAKHLHRETRSHDGSGLIREQLTFADNTVAAALYGQANGHLRII
                     ERQLGLSLHARGNELSIIGSKESVELAHRLLEQLREMQQQGRPVTTDDVVRGLQMLQH
                     DKDTAVSDVLRDAVVVANRSRPVIPKSVAQQRYLDCIRNEDVVFGIGPAGTGKTYLAM
                     AMAVKALLDKQVRRIVLCRPAVEAGEKLGFLPGTMQDKVNPYLRPLYDALNDLLDMER
                     AQLLIDRGQIEVAPLAFMRGRTLNDSFVILDEAQNTTSEQMKMFLTRLGYDSKAVITG
                     DITQIDLPTRGQSGLVEAMKLLSGIVGIEFVHFSEVDVVRHPLVAAIIRAYDRPTSNS
                     GSGT"
     gene            6737..8059
                     /locus_tag="PKL17_13420"
     CDS             6737..8059
                     /locus_tag="PKL17_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNK45781.1"
                     /translation="MFVPPQLRRFWVLLVVALCVVPSLALAAPLETDLGDRLQAALSG
                     QGGSMQLAYLLAFLGGVATSLTPCVYPLIPITIGLFGAREAPRAQALGLAACYVGGIA
                     ATYTTLGLVVGLVGGQFGTFMTSPWVIVPVAVFFLLMAASMFGAFELSLPNELQGKLS
                     QVGGQGPIGAFLMGLVAGIVAAPCTGPPLAALLLFVSTQRSVLLGGSLLFVYAIGMGM
                     LFFAIAGFALKLPKSGAWMDGVKAVFGVVMIVAALYYLRNVVPLLRMYGKSTTVFLVS
                     QAGLVVLGILVGGLTLSFADGPRAAMRKALGVLLMCVGLYGSVAWAIAPRGGLHWLTN
                     EAAAVQKAQSEGKPLLVDFGAEWCVPCKQMETKTFSNEEVRRELSRFVLLRIDCSEST
                     PENEKLQQKYQSQTLPSVLVYDRTGQRTAWLREFTPPETLLPILLKTP"
     gene            8065..8493
                     /locus_tag="PKL17_13425"
     CDS             8065..8493
                     /locus_tag="PKL17_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45782.1"
                     /translation="MATPRTSAKKPAAKTSKRDAKPADGLASAADAVAVSSAGKMKIP
                     ELLAELEETAKQLDVKVTYEAMTGELGSGGLCKVKGQWRVIIDKRATPGDRVSILALA
                     LTRVPREGVTIHPSVEKLLSEVKPRAVSMEDALDSDTPTA"
     gene            complement(8515..9231)
                     /locus_tag="PKL17_13430"
     CDS             complement(8515..9231)
                     /locus_tag="PKL17_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45783.1"
                     /translation="MGCSTCKTGCGSVDGGCSKQKNAQKQFLGRLLPRLYPNRTYRQF
                     DVVTAFDEGISESESEMLGQRIATATKAPVYFVSPSDDELCRYLYVLCIGREPPLVVL
                     RQSAQLASLEADRIRERYLRVALSSVARVACVQEVAMELDLSAGPDDQFATVRELPLG
                     GVFDPLLLKRLQRIVDLLTAYDIRHLDLGLLEQPASGFDLADAGFADRFGVEPQLANF
                     LFFAAPAVTTSVVCLPVKLP"
     gene            complement(9237..10667)
                     /gene="glmU"
                     /locus_tag="PKL17_13435"
     CDS             complement(9237..10667)
                     /gene="glmU"
                     /locus_tag="PKL17_13435"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNK45784.1"
                     /translation="MSQADSMAKPLYAIVLAAGQGTRMRSARAKVLHKILGRTMCDCS
                     LALLGPLGVSATALVLGHQADAVEASVRASGLDVPGLRIAIQTEQKGTADAVRSALPS
                     LPELAESGGKVLILYGDTPLLSVASLQELIDKTTGKLGLLSTILPDPHGYGRLIRTAG
                     KATAIVEHKDCTPDQLAVCEVNAGIYAVDAKFLLDSLSQVHKSSVTREFYLTALVELL
                     VAQGEEVTVIVAPPQEVMGVNDRADLAVAESHMRARINRGHMLAGVTLHDPLTTSIDA
                     DVQLGRDVEIFGGVQLRGKTRIGEGCIIDQGCVLTDVIVDAGVHIKPYSVAASSHIGP
                     RCQVGPFSHLRPDSDLSEDAHVGNFVELKKTQLGRGSKANHLAYLGDSTIGAQVNIGC
                     GTITCNYDGFAKHQTIIEDGVFIGSDTQLVAPVRVGKNAMVAAGSTVTREVPDGALAL
                     SRVPQENKEGMADFLRARFRARKAKK"
     gene            complement(10690..11670)
                     /locus_tag="PKL17_13440"
     CDS             complement(10690..11670)
                     /locus_tag="PKL17_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNK45785.1"
                     /translation="MRPELLSELRSSDVKWILRTARLLASDVERAASIVKTPAYTELC
                     HVGKEQAPELSSVAPDALRAQLEEMLLLDGVPLGLQWLYDSGALLALLPELHATVNFS
                     QEAGRRHKDVWEHTKQVVWQSPPRPVVRWSALLHDIGKVKTRAFTPDGKVTFHRHSEV
                     GARMFDDIGRRFQFDRDNHRSLRFLILHHLRPNAYLPSWTDSAVRRFAKEIGPYLPDL
                     LDLSRADITSRKPGRRQQGLGNIDTLSQRVSQIRAEDAVIPPLPSGIGDAIMDKFRMP
                     PSKLIGDLKRELLAKIDAGEIEPRMESEYYLPIVEQMIASRAEAPRLCCE"
     gene            11783..13603
                     /locus_tag="PKL17_13445"
     CDS             11783..13603
                     /locus_tag="PKL17_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45786.1"
                     /translation="MIGAAISTGLQTLRARALRLSQTVLLVWRAAPSLLLGLSLLTIL
                     GVLLPLAVAYVGKSLVDAVVAHNRDLALRLVGLELALMVSQALQFRAQSLTKMLLGAR
                     LSVDINVMILKKAVSLDLAHFEDPKFYDQLTRARREASSRPLGMVGDVLQLVQGVLTL
                     VGYIGLLWTFSPLAVMVLIAAALPAAAGEVRFSRAAFRLRNFRAPESRRLNYIEYILG
                     SDEHAKEVMVLGLGQTLLDRYRTLGESLYEDDRKLAVRRAIWGYLLSLLATVSFYGCY
                     AVIVGLAVSGKLSLGEMTLYVVAFRQGQQAFQSTLSSLGGIYEHDLYMSNLFDFLAIP
                     TVLSRATALLPAHAESAETGIEFDHVSFKYPGETRYALRDVSLKIPAGQSLALVGHNG
                     AGKTTFIKLLCGLYQPTEGRILLDGKDLSTLPPDAVRKRMGVVFQDFGQYQFSVRDNV
                     SFGSIAHSDDKPRIERALERGGADEFVRGLPDGIEALLGRWFHKGVELSGGQWQRIAL
                     SRAFIREEADILVLDEPTAALDAEAEHKVFERFRELTQGRTAILISHRFPTVRMADRI
                     VVLEGGSDGGHIAEQGTHSELIAQGGRYAGLFKLQAQGYL"
     gene            13606..14328
                     /locus_tag="PKL17_13450"
     CDS             13606..14328
                     /locus_tag="PKL17_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNK45787.1"
                     /translation="MAHVLIPLPDTDFDVTEVAVPWRLLTRKGHRVSFATEHGDKPPS
                     ADPLLLSGVLFGKLGAEPEPKSFYAEMIASPEFRAPLAWESLNMNHFEGLILPGGHAP
                     GMRQYLGSEVVQQKIRDFFLLKRPVGAICHGVLVAARAGVLKGHKTTCLPMYMERLAY
                     FISAWKLGTYYRTYPAYVEEEVRAGLSSPSDFVRGPITLSERGTQENDAPAFVVESDY
                     YVSARWPGDAYLFAKKFIERLP"
     gene            complement(14341..14811)
                     /locus_tag="PKL17_13455"
     CDS             complement(14341..14811)
                     /locus_tag="PKL17_13455"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNK45788.1"
                     /translation="MWWFVLKSFHVLFAMLYLGTGIGSAYYKLRAFRSGDLRVIAFFD
                     REIVIADWLFTVSSGIVMPATGLTMVLKLQLPLATSWVVTAFALWMVAGLAWLWAAFL
                     QIRMRNLSAQALGQNQPLPPEYLQAFKLWALLGIPAFLAAMATVVVMVKKPMLW"
     gene            complement(14793..>15601)
                     /locus_tag="PKL17_13460"
     CDS             complement(14793..>15601)
                     /locus_tag="PKL17_13460"
                     /inference="COORDINATES: protein motif:HMM:NF025154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HNK45789.1"
                     /translation="ACFCGKITLVFASLFPLASVDPQRAETWLRASLSAVWIWTALAV
                     LHPYYRLVGDSYLHALSLPSLLMYLTCAAELGLGIVVLVVRPVRWLVSLQAALVLSFS
                     LILALKEPLLLASPFGMLSKNLQFLCVLYVLFLVEKHGSWTDLAKRYLRIGMAVVWLT
                     EGLFPKLLFQQQIELQMVPRIGIEQISPSILVGAMGVAQILSAVLALVLSGKWLRLLL
                     LLQAGALLLLPLLVGFLEPSLYVHPFGPFSKNLPIFVGTLVVRKQCGGLS"
BASE COUNT         3167 a         4738 c         4535 g         3161 t
ORIGIN      
        1 cgcaaccgaa cgtctcagcc ggatcggtca gtcagtttct ttcgaccggt gacataaacg
       61 gtgatggcaa cgtcgacttc gtgagcaacg actttagctc gggtgcgata aacctccgct
      121 ttggaaatgg aaccggcgga ttctctccgg cccagaccat cgcaacggga ctccaggcaa
      181 ctgccgcacg atttgccgat gtgaaccaag acggcgcgct ggacatcgtc cacctgcttt
      241 acggaaccag caaaggactc gtgttcctgg gcaacggaac gggtagctat gcgaatccga
      301 cgcagtttac tgctgcgaac aatccctaca acgtcgttgt aaatgacctg aatcgcgatg
      361 gcgttcctga tctggtggtt tcaaacgacg gcagcagcaa catgacagtg ctgctgggag
      421 acggaaaagg cggcttcgca agcaacgcca cgctcgcgac cggcgcaaac ccctacttct
      481 tgttgaccgc agacctcaac aaagacggaa acatggacat cgtctcctcg gaccgcggca
      541 gcagcgtgct gtctgtctat ttggctgtcg atcagtgcaa gtaggccgcc gccactctcc
      601 aaaaaactga acgcatcgat atgggcagcg cttccgcagc agaaagttcg gcgataggtt
      661 ctgcggaaac cagttgtgca ggtctatccg tccgccatcc catcagcgcg acccagcagc
      721 caatgggtgc gcactggacc tttgcctttt aactcgatga ctccgcgagt ctcaaagaga
      781 tatcgctcac cgagtgcggc gcgcgtctca tcgctgatct ggattgcacc tggcttgcca
      841 tgggactcta gacggctcgc ggtgttcacg gtgtcccccc aaagatcgta gctgtacttc
      901 ttcttgccga tgactccggc aaccaccggg ccggtgtgta ttccgatccg catcgcgagc
      961 ccaccgtaat cgtgaatgtc cgcaatgaca cgccgcatct caagtgccat ttcagcgact
     1021 gcgcaggcat ggtccttgtt cgcagcgggc agcccaccgg ccaccatata ggcatcaccg
     1081 atcgtcttga tcttctccag gccgtgccga tccgcgattt catcgaacgc cgaaaacaca
     1141 cggtttagga gtgccagtac atcagccggt gacagtcgag cagacagcga agtgaagccc
     1201 gcgagatccg cgaacagcac ggtcacttga tcaaacgcat cggcgatcgc gcccggttcg
     1261 cttttgagcc gctgcgcgat cgaggacggc aggatgttga gcagcagcgt atcggacttg
     1321 tgccgggcgt catctagtag ctggttgcgg aagaaatccc gtttccgcga acgttcatcg
     1381 aggtagctta tgcgcatggc caaaagctgc acggcaaaac accacagtgc cgcgctgatg
     1441 acgaagggct gtggtagagg tgtcttgagc gcgatgaccc caatatagat tccgaacaga
     1501 gccacagaga tcgctgatgc cgttaggaat cgaagtccca tcatggcgtg aacaccgacg
     1561 cagtagatgg tcagaacaaa caacgaataa tcatcgaaac gccccggcag gtacgactgt
     1621 gcaatcggaa tgctgagccc gatcagaaag tcgacgaaag gcccgatgcg aaaggcaaat
     1681 gatcgcgttg cagtcggaat gcagagcagc attgcgccaa cgagcagagg cagcgccgtg
     1741 acgaacacga tgttccgcag cgtcgcgcca ccatgcgcag gcataatgaa actccacagt
     1801 acgatgagca gccacatcgt gatggccagc ccgtaggaaa tgagcgtttg cccaaccgag
     1861 cgccgcttct cacgctcatc gagatacctc cgctcgatgc gtgcatccag aaaggaaagc
     1921 gtgtagggcg aaacaggagt cgaaaccatc gccatgacgc attttatcag gtcggttcgt
     1981 ctggcgtgcc gttgcatctg tgggaccgtt acccggagat ggctccgttt cgtaaagaca
     2041 tgatttccgg actttctcgc cagcgtctta ctggtctcga tccggctggc gcagcggctg
     2101 ggttgctccc ctgtctgtct atttggctgt cgatcagtgc aagtaggccg cgtttcgtgt
     2161 gaagataggt ttcgtgcccg cgagtcagcc tgagatcctg tcaaagcttc cgccgctcgg
     2221 tccgcgcgtc cgccgaatca tcatccatgc ctgggacgga ttttgggacg atcgctgctc
     2281 actggccgca tcgtctctgt cgtatcagac tgcgctcgcg ctggttcctc ttttggccat
     2341 cgcactcgcc ctgctgaaat acggaggcca gctcgaagac ggctcgcgac tgctatccgt
     2401 cgtcgtgcat caggtgtttc cgtcttccca ggaagcccaa gccgaagtcg tcaatcgact
     2461 gtccacgttt tccggcaact tcaccgcggg aggactcggc agctttggca tcgtcgcggc
     2521 gctggcggtt ggtttctttc tgttcctctc tgtggaatcg atctggaatc acatctggga
     2581 atcacatcgc gagcgcacgt atgtcgaacg attccttttg ttttacaccg gcatcacgct
     2641 gattccgttt gtcgtcacgc tgtcgtttgt gcagacagaa aaactgtggg gcagctttgc
     2701 ggtggttctg ccctatctgt cgccgctgct gacgacggta atgttgacgc tggcgaaccg
     2761 actgattccg acgctgcggg tgcgctggcg tgcagcgctc gctggaggcg caaccgcagc
     2821 ggtgctgctc gaaatcagca agttcgcact gggccgttat ctgacctggg ttgcgacaag
     2881 ctacaagtcg atttacggcg cgctcggtgt cctgccgctg ttccttttgt cgatttatct
     2941 gtggtggctg atcgtgctgt ggggcgtcga ggttgcacgt tccgtacagc ggctgccgtg
     3001 gcaactgcga attcacgatg acgcgaaccc gcatgacgcg cagctcacca tcaatggacc
     3061 gctcgcagtg cggctgctca ccgacgtagc gcgacacttc aaaaacggac aaaagagcct
     3121 gcccgtggat gaaatcacac agcgacacgg actgccggaa agcattgtcc gcaaaatcct
     3181 aaaccggctc gccaagacgg gatttgtcgt cgagcacgaa gacggttatc tgctcgcgcg
     3241 gcccccggac ggaatccgtg tcgaagaagt gctgcggctg ttccaaccga cccagtatct
     3301 gcatgccgat gtggaacccg atcagctcga tcaagtgctc gccaagctcg aaaagcagca
     3361 gcagcaagtc ctggccgaca ccacgctggc caccctgatt taaggggccg gaggcggctg
     3421 ggccccgtcg cggtattcca gcctcgagtc cacttcgccg ctttcacgga agaacgtcca
     3481 caccccgtca cgccgtcctt cgcgatacat tcccgccgat tttttcattc cgttttccca
     3541 ccagtcgacg tactcgccgt gacgggttcc atcgggacgc tgacaccacg ccgcggtgta
     3601 cgtttgaaac gacgtgccac gcgaaagctc ggtgcctttg ccgcagctca cagcgcgcgt
     3661 cgaatcggtg attttggcag gttccgacag aggcggacct ggatgagcgc agccaaaccc
     3721 aagcgaacaa acaagtgaaa gacacacagc gcgcaagtac atagcaagga gcctagcaca
     3781 gccccaacgt cgaccaagtg atcgcatctg gtcggacttg cggaccgtgc ctccgtccac
     3841 gcatgatcgt ctgcatcagc gctgaggagc gaagatgcgg ggtcgactgc tgcttgtgtt
     3901 gtgcctgctt ttgctgtgcg cgatgccttc acttggctgg gctttcgatc ctgcctttgc
     3961 ggtgccagtc ttgcggccca gcgctggtgc gtcgtggatc gtgagcggcg aaagagaaga
     4021 cggctttgcg ctgcatgttc ccgtcggagc actcgttggt tgggcgccaa atggactggc
     4081 tcggctgttc gatcggacgc cgtttttgct gcccgaactt ggctacgagc tgcgcgtgac
     4141 caaaaaccat cgcagcgacg ctttttcggc tggacttggc gtcggctacg gcgtgctcac
     4201 actcgcgatt gtttcgtaca caccgcgttt cactgtgggg tcaagcgacg gacagaccgc
     4261 gctcggtttt cggcacagcg ttgcagggca cttcctgctg tcggtgattt atctcgaact
     4321 gtcccacgaa gtgctacggg cgggatccat caccagccac gacattcaac tgtccgccgg
     4381 gcttaacgtg ggaacgctgc tgattccgct gtttttctga cgagacagcg gacgttgcca
     4441 gcaggctgtg taacgtcccc agcagcgact ctttgcgtgc tcagcgggac ccaaaaacga
     4501 caacgatccg acctgttgct gatccgccga aacctgctac agattgcgca tgtcggattc
     4561 ctcttatgct gacgcgctga aaaaacgcct gtggcggcgc ttgatcgtcg cgacgatggc
     4621 ggcggctgca ctgtttgccc attcgtgcag cgacatcacc accagcacac ttttgtgtga
     4681 ggacgccgcg tcacgcatct tcgagtgctg cagtgcccac ccggcggtaa gctgccacga
     4741 aaatggctgc gctgaccagc gaccgaactt ctcccaagct accgccaagt gtattcgctc
     4801 ctcgtcttgc gacgcactgc gtcaagccgg aatctgcgat gtcaccgact ggcacatgcc
     4861 aagctacgca agctgcatcg acggccaagc gatctgccaa aacgacggtg gcgtctgccc
     4921 cgcatcggtt gcgccagcct gtgccgcact cgggaggctg acatgttcct aaaatccatc
     4981 ctggtcgccg ccttgctgtg tggcgcgacg ccatcggcgc ttgcgcaaat cacgcccgat
     5041 gccccacagg cgccgcagct cggccagcca ctgaccgaag caccgccgtc gctgccaccg
     5101 cccgctgtcg tcagttcaga gccagggcaa gcgcgctttc acaaaccggc atttaccatg
     5161 gcggtcgtcg gaggcgcgtc gtttcagtac atcatcgaaa cgccgttttt gagcggcggt
     5221 gccgaactgc gcttgggatt catgacccgc aaagtcgaat tcacgacgcg gctgcgcatg
     5281 ctggctgggt cgactctcgc gggacttgtg ctgttggcac cgtcgataag cgctggggtg
     5341 atggtgccga tcagtcagcg cgttcgcatc ggcgccgaag tgacgctgcc gccgtttgtg
     5401 cgcggaatgc tgattcgtta cgtcacccaa agcgacatgg gccgcgcagc tctcgtcggt
     5461 gctggttttg aaaccagcgt cgacatcgtg caatggaaag acaaccgcgc gctgttttgg
     5521 gtcggcacac ttggcgtcga cgtcacggcg gtgcgagtga acggcgacag cggccaaacc
     5581 gccagtgtcg gtccgaatct ctttacgggc atcggctatc gcatctaagc tttcgccttc
     5641 ttttttaggc cgctatggca aaacaccttc atcgtgaaac gcgcagccac gacggcagtg
     5701 gtctgattcg cgaacagctc acgttcgccg ataacaccgt cgctgctgcg ctgtatggac
     5761 aggccaacgg gcatcttcgc atcatcgaac gccagcttgg cctgtcgctg cacgctcgcg
     5821 gcaacgaact gtctatcatc ggcagcaaag aatccgtcga gctggcgcac cggctgcttg
     5881 aacagcttcg tgaaatgcag cagcaaggcc gtccagtgac caccgacgac gtggtgcgcg
     5941 ggctgcaaat gcttcagcac gacaaagaca cggcggtgtc cgatgtgctg cgcgatgcgg
     6001 tggtcgtcgc caatcgcagc cgtccggtga ttcccaaaag cgtcgcccag cagcgttacc
     6061 tcgactgcat tcgcaacgaa gatgtcgttt tcggcatcgg gcccgcgggc accggcaaga
     6121 cctacttggc gatggcgatg gcggtcaagg cgctgctcga caagcaagtc cggcgcattg
     6181 tgctctgtcg gccagccgtc gaagcgggcg aaaagctcgg gtttttgccg ggcaccatgc
     6241 aagacaaggt caatccctat ctgcgaccgc tgtacgacgc gctcaacgac ttgctcgaca
     6301 tggaacgggc gcagcttctt atcgatcgcg gacagatcga ggtcgcgccg ctcgccttta
     6361 tgcgcggacg gacactcaac gattcatttg tcatcctcga cgaagcgcag aacacgacga
     6421 gcgagcagat gaagatgttt ttgacccggc tcggttacga cagcaaagcc gtgatcacgg
     6481 gcgacatcac ccagatcgac ttgccgacgc gcggtcagag cggtctcgtc gaagcgatga
     6541 agctgctcag cggcatcgtc ggcatcgagt ttgtccactt tagcgaagtc gacgtcgtgc
     6601 gccatccact ggtcgccgcc atcatccgcg cctacgatcg tccaaccagc aacagcggca
     6661 gcggcacgta acggcgctgg cgatgcactc gtactagaaa gcagccgcat tccaacttca
     6721 ggggtataac gaaaccatgt ttgttccccc acagcttcgt cgcttctggg tcttgctcgt
     6781 cgtcgcattg tgtgtggttc ccagtctggc gctggctgca ccgctcgaaa ccgatctcgg
     6841 cgaccgactg caagccgcgc tgtctgggca gggcggttcg atgcagcttg cctatctgct
     6901 ggccttcctg ggcggcgtcg ccaccagctt gacaccgtgc gtctatccgc tcattccgat
     6961 cacgattggt ctgttcggtg cccgcgaagc gccccgtgcc caagccctgg gtctggccgc
     7021 ttgctacgtc ggaggcatcg ccgcaacgta caccacgctc ggtctggtcg tcggtttggt
     7081 cggcggacag tttggcacct tcatgaccag cccatgggtc atcgtccccg tcgcggtgtt
     7141 tttcctgctg atggcggcgt cgatgttcgg tgctttcgag ctgtctttgc caaacgaact
     7201 gcaaggcaag ctgtcgcaag tcggtggcca aggtccgatc ggcgcgtttt tgatgggcct
     7261 cgtcgcgggc attgtcgcgg cgccttgtac gggtccaccc cttgccgcgc tgcttctgtt
     7321 tgtgagcacc cagcgatccg tgctgctcgg tggttcgctt ttgttcgtct atgcgatcgg
     7381 gatgggcatg ctgtttttcg ccatcgctgg ctttgccctg aagctgccga agtcgggcgc
     7441 gtggatggat ggcgtcaaag ccgtgttcgg tgtcgtgatg atcgtcgcgg cgctgtacta
     7501 cctgcgcaac gtggtgccgc ttctgcgaat gtatggcaaa agcacgacgg tctttttggt
     7561 gtcgcaggcg gggctggtcg tactggggat cttggttggc ggacttacgc tgagctttgc
     7621 cgacgggccg cgtgctgcga tgcgaaaagc gctcggggtg ttgctgatgt gcgtcggtct
     7681 gtacggcagc gtcgcttggg cgattgcgcc acggggcggg ctccactggc tgacaaacga
     7741 agcggcggcg gtgcaaaaag cccagtccga aggcaagccg ctgctcgtcg actttggcgc
     7801 cgagtggtgt gtgccgtgca agcagatgga aaccaagacg ttttcaaacg aagaggtgcg
     7861 gcgcgagctg tcacgctttg tgcttctgcg catcgactgt tcggaatcga cgcccgaaaa
     7921 cgaaaagctg caacaaaagt atcaatcgca gacgctgccg tcggtgctgg tgtatgaccg
     7981 aaccggccag cggaccgcct ggctgcgcga atttaccccg cctgaaacgc tgctgccgat
     8041 cctgcttaag acaccgtaac gacgatggcc acgccccgta cttctgccaa aaaacctgcc
     8101 gccaagacca gcaagcgcga tgcaaaacct gccgacggtc tggcatcggc agcggacgcc
     8161 gtcgcggtga gcagcgccgg gaagatgaaa attcccgaac tgctcgccga actggaagaa
     8221 accgccaaac agctcgatgt gaaagtgacg tatgaagcga tgaccggcga gctgggttcc
     8281 ggcggtctgt gcaaagtcaa aggccagtgg cgcgtcatca tcgacaagcg agcgacgcca
     8341 ggggatcggg tgtcgatttt ggcgctggcg ctgacccgcg taccccgcga aggggtcacg
     8401 atccatccgt cggtggaaaa gcttctttcc gaggtcaagc cacgcgccgt ttcgatggaa
     8461 gacgcgctcg attccgacac gccgacggcg tagcccggct ggcgctgacg atggttacgg
     8521 cagcttgacg ggcagacaca ccacgctcgt cgtcacggcg ggggcagcaa aaaacaggaa
     8581 gttggcaagc tgcggctcga cgccaaaccg gtcggcaaag cccgcatcgg ccagatcgaa
     8641 tccgctcgcc ggctgttcca gcagtcccaa atccaggtgc cgaatgtcat atgccgtcag
     8701 caaatcgacg atgcgctgaa gccgcttgag caacagcgga tcaaacacac cgccgagcgg
     8761 aagctcgcgc accgtcgcaa actgatcgtc cggccctgcc gacaagtcca gttccatcgc
     8821 gacttcttgc acgcacgcaa cccgcgccac cgacgacagc gcaacccgca gatagcgttc
     8881 gcgtattcga tccgcttcca aggaggcaag ctgcgccgac tgcctgagca ccacaagcgg
     8941 cggctcgcgc ccgatgcaca gcacatacag atagcggcac agctcgtcat ccgagggcga
     9001 cacaaaatag accggcgctt tcgttgccgt cgcaatgcgc tggcccaaca tctcgctttc
     9061 ggattccgaa atgccttcgt caaatgccgt caccacgtcg aactgtcggt acgtccgatt
     9121 tggatacagc cgtggcagaa gccgacccag gaactgcttt tgtgcgttct tctgcttcga
     9181 gcaaccgccg tcgaccgatc cgcagcctgt cttgcacgtc gaacaaccca tgtcgattac
     9241 ttcttcgctt tgcgagcccg aaagcgtgcg cgcaaaaagt ccgccatccc ttctttgttt
     9301 tcttgcggca cccgcgacag cgccaatgct ccatcgggaa cttcgcgcgt gacggtcgat
     9361 ccggcggcca ccatcgcatt ttttccgacc cgcaccggcg ccacaagctg ggtatccgaa
     9421 ccgatgaaca cgccgtcttc gatgatggtc tggtgtttgg caaagccgtc gtagttgcag
     9481 gtgatggtgc cgcagccgat gttgacctgc gcgccgatgg tgctgtcgcc cagataggcc
     9541 aggtggttgg ctttgctgcc gcgaccaagc tgggttttct tcagctcgac gaagtttccg
     9601 acgtgagcgt cttcagaaag atcgctgtcc ggtcgcaggt gcgaaaacgg gccgacttgg
     9661 cagcgcggtc cgatgtggct cgatgcagcg acggaatatg gctttatgtg aaccccggca
     9721 tcgacgatga catcggtcaa aacgcagcct tgatcgatga tgcagccttc gccaattcgc
     9781 gtcttgccgc gaagctgcac gccgccaaaa atttcgacgt cgcgcccaag ctgcacatcg
     9841 gcatcgatgc tggtcgtcag tggatcatgc agcgtcaccc ccgcgagcat gtgcccacga
     9901 ttgatccgcg cccgcatgtg gctttccgcg acggccagat cggcccggtc atttacgccc
     9961 atcacttctt gcggcggcgc gacaatcacc gtcacttctt cgccttgcgc gaccaacagc
    10021 tcgacgagcg ccgtcagata aaactcgcgc gtcaccgagc ttttgtgcac ctggctcagc
    10081 gagtcgagca aaaatttggc atcgacggcg taaatccccg cgttgacttc gcagaccgcc
    10141 agttgatccg gcgtacagtc tttgtgctcg acgatggcgg tcgcttttcc agccgtgcga
    10201 atcagccgac cgtagccgtg cgggtcggga agtatcgtcg acaaaagccc cagcttgccg
    10261 gtcgttttgt cgatcagttc ttgcagactg gccaccgaca gaagcggcgt atccccatac
    10321 aggatcagca ccttgccacc cgattcggcc agctctggca gcgatggcag cgcactgcgc
    10381 acagcatcgg cggttccctt ttgttcggtt tggatcgcaa tccgcagacc cggcacatcc
    10441 aagccacttg cgcgcaccga cgcttcgacc gcatcggcct ggtgcccaag cacgagcgcc
    10501 gtcgctgaca cccccagcgg acccagcagc gcaagcgaac aatcacacat cgtgcggccc
    10561 aagattttgt gcagcacttt ggcccgcgcc gatcgcatcc gcgtcccttg accggcggcc
    10621 agcacaattg cgtacagcgg tttcgccatc gagtcagctt gtgacatcgt tttttcctcg
    10681 cgaggggggt tattcacagc acaggcgcgg cgcttctgca cggctcgcga tcatctgttc
    10741 gacgatcggc agatagtatt cggactccat ccgcggctcg atttccccgg cgtcgatttt
    10801 ggccaaaagc tcgcgcttca gatccccaat cagcttcgac ggtggcatgc ggaacttgtc
    10861 catgatggcg tcgccaattc cgctcggcag cggcggaatt accgcatctt cggcgcgaat
    10921 ctggctcacc cgctgcgaca gcgtatcaat gttgccaagt ccctgctgcc gtcgacccgg
    10981 cttgcgcgac gtgatgtcgg cgcgcgacag atcgagcaga tcgggcagat aaggaccgat
    11041 ttccttggca aaacggcgca ccgctgaatc ggtccacgac ggcaaatacg cattgggccg
    11101 caggtgatgc aggatcaaaa accgcagcga gcggtggttg tccctgtcga actgaaagcg
    11161 ccgtccgatg tcatcgaaca tccgcgctcc gacttcgctg tggcgatgaa acgtcacttt
    11221 cccgtcggga gtaaacgccc gcgtcttcac tttgccaatg tcgtgcaaaa gcgccgacca
    11281 gcgaaccacg ggccgcggcg gcgactgcca gaccacttgc ttggtgtgtt cccagacgtc
    11341 tttgtgacgg cgaccggctt cctgggaaaa gttcaccgtc gcgtgcagct ccggcaaaag
    11401 cgcaagcagc gcgccgctgt cgtaaagcca ctgaagtcca agcggaacac cgtcgaggag
    11461 tagcatctct tcgagctgcg ctcgcagcgc gtccggcgcg acactggaca gctcgggcgc
    11521 ttgctctttg cccacatgac agagctcggt gtaggccggc gttttcacta tcgaagcggc
    11581 tcgttccacg tcggacgcca gcagacgagc ggtacgcaaa atccacttca catcgctgct
    11641 acgcagctcg gataacagtt caggacgcat gttctattcg aggatgggcc gcggtcgtcg
    11701 cgcagcacgg tccgtcaggc tacgaagctt gcgaaacgtc gtcaaacagt tttgctacgt
    11761 cgccgtgcta gcatgcgaag cgatgatcgg agcggccatt tcgacgggtt tgcagacgct
    11821 gcgggcgcgg gctctgcggc tttcccaaac ggtcttgctg gtatggcgtg cggcaccttc
    11881 gcttttgctt gggctgtctc tgctgacgat cctgggcgtg ctcttgccgc tcgcggtggc
    11941 ctacgtgggc aagtcgctcg tcgatgcggt ggtcgcgcac aaccgcgatc tggcgctgcg
    12001 gctcgtcgga ctagagcttg cactgatggt gtcgcaggcg ctccagttcc gcgcccagtc
    12061 gctgaccaag atgctgcttg gcgccagact ttccgtcgac atcaacgtga tgatcttgaa
    12121 aaaagcggtg tcgctcgact tggcgcactt cgaagatccg aaattttacg atcagctgac
    12181 gcgggcccgg cgggaagcgt cgtcgcgacc gctcggcatg gtcggtgatg tgcttcagct
    12241 tgtgcagggc gtccttaccc tcgtcggcta catcggtctt ttgtggacgt tcagcccgct
    12301 cgcggtgatg gtgctgatcg cggcggcgct gccagcggcg gcgggcgaag tgcgcttttc
    12361 gcgtgccgca ttccgtctgc gcaacttccg cgcgcccgaa agtcggcggc tcaattacat
    12421 cgaatacatc ttgggcagcg acgagcacgc caaagaagtg atggtgcttg ggcttggaca
    12481 gacgctgctc gatcgctatc ggacgctcgg ggaatcgctg tatgaagacg atcgcaaact
    12541 ggcggtgcga cgcgcgatct ggggttatct gctttcgctg ttggcgacgg tttcgtttta
    12601 cggctgttac gcggtgattg tcggtttggc agtttcgggc aagctcagcc tcggtgaaat
    12661 gacgctgtac gtggtcgcat tccgccaagg tcagcaggcg tttcaaagca cgctcagcag
    12721 cctgggaggc atctacgaac acgatctgta catgtccaat ctgttcgatt tcctggcgat
    12781 tccgacggtc ctaagccgtg ccaccgccct gcttccggcc cacgcggaaa gtgcagaaac
    12841 cgggatcgag ttcgatcacg tcagcttcaa atatccgggt gaaacccgtt atgccttgcg
    12901 cgatgtcagc ctaaaaattc cagccggaca gagcctcgcg ctcgtcggac acaacggtgc
    12961 gggcaagacc acgtttatca agctgttgtg cggtctgtat cagcccaccg aaggcaggat
    13021 tttgctcgat ggaaaagact tgtcgacgct gccgccggat gcagtgcgca agaggatggg
    13081 cgtcgtgttt caggactttg gtcagtatca gttttccgtg cgcgacaatg tgtcgtttgg
    13141 cagcattgcg cattccgacg acaaaccacg gatcgagcgg gcacttgaac gcggcggtgc
    13201 cgatgaattt gtacgcggtc ttccggacgg aatcgaagcg ctgctcggtc gttggtttca
    13261 caaaggagtc gagctgtccg gcggacagtg gcagcggatt gcgctgtcgc gggcgtttat
    13321 tcgcgaagaa gccgacattc tggttctgga tgaaccgacg gcggcgctcg atgcggaagc
    13381 tgaacacaag gtctttgagc gattccgcga gctgacccaa ggtcgcaccg cgattctcat
    13441 ttcccatcgt tttcccaccg tgcgcatggc cgataggatt gttgttttgg aaggcggcag
    13501 cgacggtgga cacatcgccg aacaaggtac gcacagcgaa cttatcgcgc agggcggtcg
    13561 ctatgccggt ctgtttaagc tgcaagcgca ggggtatctg taaccatggc tcacgttctg
    13621 attccgcttc ctgatactga ttttgatgtc acggaagtcg ccgttccgtg gcgattgctc
    13681 acccgcaaag gacatcgcgt aagctttgcg accgagcatg gtgacaaacc cccgagcgcc
    13741 gatccactgc ttctgtccgg cgttctgttt ggcaagctcg gtgcagaacc agaacccaag
    13801 tcgttctatg ccgagatgat cgcatcgccg gaatttcgcg caccgctcgc ttgggaatcg
    13861 ctcaatatga atcattttga aggactcata ttgccaggcg gacatgcccc tggaatgcgt
    13921 cagtatttgg gcagcgaagt cgttcagcaa aaaatccgtg acttcttttt gttgaaacgc
    13981 cccgtcggtg cgatctgtca tggcgttctg gtggcggcgc gggccggcgt gcttaaaggc
    14041 cacaaaacca cctgcttgcc gatgtatatg gagcgtcttg catacttcat atcggcgtgg
    14101 aagctcggta catactatcg aacctatccc gcctacgtcg aagaggaagt cagggccggt
    14161 ctttcgtcgc caagtgactt tgtgcgcgga cccatcacgc tgagcgagcg cggcacgcag
    14221 gaaaacgatg cgccagcttt tgtggtcgaa agcgactatt acgtatcggc acgatggcct
    14281 ggggacgcct atctgtttgc gaaaaaattc atcgaaagac tgccgtaacg ccctgcccgc
    14341 ctaccacagc attggctttt tcaccatcac aaccaccgtc gccatggccg ccaaaaaggc
    14401 cgggattccc agcagcgccc acagcttgaa tgcctgcaaa tattctggcg gaaggggctg
    14461 attttggcct agcgcttgcg ccgacagatt gcgcattcgg atctgaagaa acgcagccca
    14521 aagccacgca aggcccgcca ccatccacag cgcaaacgcg gtcacaaccc acgatgtcgc
    14581 aagcggcagt tggagcttta acaccatggt aagtcccgtc gctggcatga cgattccgga
    14641 gcttactgtg aacagccaat cggcgatgac aatttcgcga tcgaaaaaag caatgacgcg
    14701 cagatcacca cttcggaagg cccgcaactt gtagtacgcg ctgccgattc cggtgcccag
    14761 atacagcatc gcaaacagca catgaaacga ctttaggaca aaccaccaca ctgcttcctc
    14821 accaccagtg ttccgacgaa gatcggcagg tttttgctaa acggaccgaa gggatgaacg
    14881 tacagactgg gttccaaaaa acccaccaac agcggcaaca gcaaaagcgc cccagcctgc
    14941 agaagcagca aaagccgcag ccattttccg gacagcacaa gcgcaagcac tgcagacaga
    15001 atctgcgcca cacccatcgc accgacgagg atggagggcg atatctgttc gattccgatt
    15061 cgcggaacca tctgaagttc gatctgctgc tgaaacagaa gtttgggaaa cagaccttcg
    15121 gtcagccaca ccaccgccat tccgatccgc aaatatcgtt tcgccagatc cgtccacgac
    15181 ccatgttttt cgacgagaaa cagcacgtac agcacgcaca aaaactgtaa gtttttcgac
    15241 agcatgccaa acggactggc caacagcagc ggctctttca gcgccaagat cagcgaaaac
    15301 gacagcacca gcgcagcctg aagcgacaca agccagcgca caggccgaac gacaagcacc
    15361 acgattccca gcccaagctc cgccgcgcac gtcagataca tcagcagcga agggagagaa
    15421 agcgcatgca agtaggaatc accgaccaag cggtaatacg gatgcagaac cgcaagcgcc
    15481 gtccagatcc acaccgccga aagcgacgcc cgcagccaag tctctgcccg ctgcggatca
    15541 acagaagcca aaggaaacag gctcgcaaac accaacgtaa tcttaccgca aaaacaggcg
    15601 g
//
LOCUS       DAORGN010000119        15557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1557_length_15557_cov_5.282867, whole genome shotgun sequence.
ACCESSION   DAORGN010000119 DAORGN010000000
VERSION     DAORGN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_15557_cov_5.282867"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="PKL17_13465"
     CDS             <1..1518
                     /locus_tag="PKL17_13465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45790.1"
                     /translation="GTLAVPPPILWTCELSEGGGQLICHDAPMQGQTLSHVLRLPASE
                     PLCSPELFARLAAGETVALTVSSNRPSVVEDITTPWPLRLTLRKLALPKPVDGGGLLL
                     TVAVEDLSFSQQIRRESLLAKERLAKVMRSVGDAILTLDPKAVIQQVNDASQKVLGLS
                     PDELCGKSVLDLAATPRARLQLEELLRHLPHTGFAEAELRLKRPTSRKSTRFLGHVSA
                     LLLGDDSGLPAGAVFRVHDQTERKRDDAERQALRLRLLQSERLSALGEMAARIAHEVR
                     NPLVSIGAAAQVIAEELPQDSPVRPEAVAIGNEVQRLDRILQNVLRFARPTRASVQRT
                     DVVAVLREVVDLLRGKARGLTLRVDVPESPPGTGVAALIDGDQLRQVLWNVIINACEA
                     STPGDPNRSLIECTVRKQRGGAPQDRPVLITVADSGAGISPAARRRVFDPFFSTKTRG
                     TGLGLAISKQIIEEAGGRIRLLNRQSGGTRVVIELRVTPYPDSNPSSGRGRPLAQ"
     gene            complement(1487..3049)
                     /locus_tag="PKL17_13470"
     CDS             complement(1487..3049)
                     /locus_tag="PKL17_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNK45791.1"
                     /translation="MRWLVRGDIDGFFGLALDNLVQLLLINTLCKYVLELPDELLYGR
                     ILPGAAVSLLLGNIFYSRQALKLAAETGRTDVCALPYGINTVSLLAYVFLVMLPTKIS
                     AQAAGHPDPARLAWHAGLTACLGSGVITLLGSLFAEKVRKATPRAALLSTLSGIALGF
                     ISLGFLFRTFAHPIVGLATLSIVLLTYFGRVKFKGGLPGGLVAVGIGTALAWATKLAP
                     VAQPVMKMGLHLPIPVVGDLFPALGQGLLWRFLPVIVPIALFDLIGSLQNVESAEAAG
                     DSYPTRPSLAVNGLGAIVAALFGSCFPTTIYIGHPGWKALGARAGYSILNGVFATLVC
                     LTGTLGYIAWAVPIDAGMAIVLWIGIVITAQSFQATPKEHAPAVVLGILPGVAAWGSL
                     LAKTGLRVGGYGSPGGPVFSDKLYAAFHASDTFIEGAFALEQGFIFTSMLLAAATVEV
                     IERRFERASMWCLAGAAVSATGLIHSYGFGPADTVVKLQPAWPFVGAYFAMAVIFMVA
                     RKVTEPMDGPGH"
     gene            complement(3115..4602)
                     /locus_tag="PKL17_13475"
     CDS             complement(3115..4602)
                     /locus_tag="PKL17_13475"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNK45792.1"
                     /translation="MTQSREKRGISRRRALKHLGVSLSALSVGCVGGESMIPLGDETG
                     GPDAYASPPDLAMPKDLDELLPQTPKELLAGIDAVVVLMMENRSFDHYLGTMQSDARY
                     PGSRRPSGLSGNESNPDGKGNLIKVAKLLNYEPKDPPHGWDSCHTQFGGGKNDGFVKE
                     YIARHGEKYGPDVMGFYDRSQLPYYYALADRFTMCDRWFASVMGPTWPNRFYLHAGSS
                     GGKKDNTGFVTGGPTTLWERMKDKGFVGKNYIAGAAAWYWGAYPGKMLQINPSAKLDE
                     FFKDCKNGTLPPFSIIDPDFLANDDHPSHNVQLGQALIATLLTAVFQSPQWSRTLFVI
                     TYDEHGGFYDHVAPPTLPDDNPDFAQVGFRVPTLLIGPTVRKGFVDSTQYDHTSVGAT
                     LRTRFGIANTSKRMAAAADISAAIDPRLAKHPSPPPSDLPRLSLRVSDFAPQLGFDSQ
                     PELTDAVKRLPSHLIRSFADDRSVLLRNLAWLRYGESLGAIEIVD"
     gene            4790..7018
                     /locus_tag="PKL17_13480"
     CDS             4790..7018
                     /locus_tag="PKL17_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNK45793.1"
                     /translation="MGIVCESQVCQVTVHARGAIVTRRVEVPQTTALPDGDIELVIPE
                     VTARCEAGSFRAQLDPGRRIVLQVESVLEPPKSPVQTGASQAKLDELKLKMDRLQAEI
                     ERLGTQRQRLAKQFPDPALRTTSLVEKVDERIADAIATAGLLAEVQKQLGDKQQLLEQ
                     QLADLAQEKSAAELRHSQAPSSEKQGRSQPTRRVTVRMTGAGPVSGLLISYVVPAARF
                     FPTYTLRLLDGGKRASWSIDAHVAQISGEDWNGVRLSLSTADLIFDARLPELPSLRFG
                     RAQPPARRGYRPAPDGLERLFAGYDRGFGPAAPTPTDGMLAESSLVPSPPDRTPLTDA
                     LHFDDSLSDEPTWATRAETADAELAKKGGPPPLEVAEYAAPPPPPRGAPLPPPSAMFA
                     MAPPAPQAPAPSRFQSAPARSGGAASLGAEPKRKRMAKEAPGGGGGGYDDEGGAGFDS
                     QGAVPSFDEAAPPDGIEPQDAWLDFDQLQLSGPEDQMFRGKLKRRSDDQSRSKLQQAV
                     RTIEKLQAPTGLIDPIVSRGQFDHRYDAEGLIKIPSDGKLHRVAILTAEGSPHLHLIT
                     VPRERADVYREAELKNPCDAPLLSGPVEVFVDGGLLTTDSISHVDKGGSVRVGMGCED
                     RVRVARNVRAEEESTGLLGGSTAMTHTVSIEVSSSLGQPIQIDVLDRMPVSEDKGLTV
                     EMVYEKPPHTPYDQAERGAPIRRGMRWRMPIPAGGKVKIEYQYRIGFSAKSEIIGGNR
                     RD"
     gene            7011..8597
                     /locus_tag="PKL17_13485"
     CDS             7011..8597
                     /locus_tag="PKL17_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNK45794.1"
                     /translation="MTNGSQQTEVQRIELDTKPLRVTLFEDRAEVVRQAKAQLPTGTV
                     MVSLRGITPLVDDQSVQPMLSGTVKLLSSQVTRRSHEAPTGSETELSESETDQKQASA
                     RRVAAERALEVAQVHEGRTSALLLAWIVALGRVPQGAATGLAGWRAALTQLLSAQTGA
                     LDQLSAAQKEILAAKQDEKRAQLRYQAARRLRPRRESTIDLQVEVPQTTVVTVEVTYI
                     TPCALWRPEHLARLSQNPDGTAVLQVVTSAVVWQRCGEEWNGVVCRFSTARPAQHASP
                     PLLSEDQLRLRRKTDAERRTIIVEAREQSIAVAGLGRGSQKVEEMPGVDDGGEPLTFE
                     ASRPVQILSTGQPVRIEIAQVTMPAQVERVVFPERGMAAHLRATATLSGPRPLLAGPV
                     VLLRGSELCGRGRTNFVGRGEPFELGFGPDDGIRVRRQTSEEREQAAITGTQRIARTV
                     KLFVSNLSGRSRKLTITERVPVSEIREVEISVEKTSGMRWDGKDGFAHFDVEVPGRAT
                     KELLLSYHIEAPSRVQLPPA"
     gene            complement(8719..8946)
                     /locus_tag="PKL17_13490"
     CDS             complement(8719..8946)
                     /locus_tag="PKL17_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45795.1"
                     /translation="MRPPLASPPLPLPGSLGERCRAKLWLLLLQRVVEAVNQRGMCAV
                     ELGRLADLPSDEIVSLQSRCGCPSCFQKRAN"
     gene            9083..10099
                     /gene="deoC"
                     /locus_tag="PKL17_13495"
     CDS             9083..10099
                     /gene="deoC"
                     /locus_tag="PKL17_13495"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNK45796.1"
                     /translation="MSSHDPQMEQLVAEVARRVAARLGGSQPLVQLGTAPSLREQPCP
                     DGPSENCTACGLCTVRRPEAVQNIVSEGASRVGSGLGTGQTPDSIAQMIDHTLLKADA
                     TAAEVEKLCSEARTYRFASVCVNSGFVPLAKRLLRGSGVMVCAVVGFPLGAMAPNAKA
                     FEAREAVRAGAEEIDMVINQGALKSRDYALVHEDIQKVVEASRPAKVKVILETGALNQ
                     EEKVVAISLSKVAGAHFVKTSTGFGPGGATVEDIALMRKLVGPDMGVKASGGVRSCDD
                     AQKMKAAGASRIGASASVAIVKGCSTPAPSEAPSKYPNRPWLANRQAKPSAAAAPTGK
                     PSAY"
     gene            10163..11359
                     /locus_tag="PKL17_13500"
     CDS             10163..11359
                     /locus_tag="PKL17_13500"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNK45797.1"
                     /translation="MTNAEAPFDRVVLIVLDSCGCGSSPDADRYGDAGANTLGHIGER
                     VGGIRLPNLAALGLGNVTQIPGVPPVDKPLGGFGRMRESSRGKDTTTGHWELAGLDVQ
                     SEFPTYPSGFPADMIARFSQATGRGVLGNRPASGTTILDELGPEHLRTGSLIVYTSAD
                     SVFQIAAHEQVVPLDELYRACEVARKLCDEKNISRVIARPFVGEPGRFKRTYNRRDYA
                     MPPPQETVLSRLASSGQPVVGVGKIWDIFAGHGITDNIHSEGNTDGLRLTLDALSQTK
                     RGLIFVNLVDFDMLYGHRRDPQGYARALVEFDEFLPSLHSKLTPRDLVLLTADHGNDP
                     TWTGTDHTREDVPLLAFTTTAPSPQTADMGLRSGFFDVAQTLCDAFALPAWSRGKSVL
                     PHLLPS"
     gene            11401..12732
                     /locus_tag="PKL17_13505"
     CDS             11401..12732
                     /locus_tag="PKL17_13505"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNK45798.1"
                     /translation="MATYNPVEIIRKKRDGNVLSSDEIRQFVSDYAQGKLPDYQAAAL
                     LMAIYFTGMSEGELLALTDAMLRSGEVISLGDVPGLKIDKHSTGGIGDKISLHLAPAV
                     AACGVKVPMISGRGLGHTGGTLDKLESIPGFSTRLSVQEFRTVLRRAGLSLIGQTDTL
                     VPADKALYALRDVTATVESIPLIAASIMSKKLAEGIDGLVLDVKVGSGAFMKTRDAAY
                     QLASTLVAIGRRAGKQVTAFLTNMDQPLGRYVGNAAEVIETLQVLEGGGPADLIELTR
                     VLGGEMLRLGGVVASEADGQGRILEVLQNGEALQRLQRCAAMQGAMIDLRDQSEPWRQ
                     NCLHPYPVYVVAPEAGYVSSIDAEAVGWASVAIGAGRERKEDTIDPAAYIYLDRKVGE
                     LVEAGEPLCHFACSADWPEGRRERAHALLSGAYAISMTPPTEKPLILDVLR"
     gene            12729..13619
                     /locus_tag="PKL17_13510"
     CDS             12729..13619
                     /locus_tag="PKL17_13510"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNK45799.1"
                     /translation="MTDRHSHSSHLATYEAVQQSATRIRNFMPAGAAPKVALVLGSGL
                     GSYADSLTEAHSIDYREIGFPSSSVHGHAGKLVYGRAAANPSTGNRGVEVLLMQGRVH
                     TYEGHSMQQVVLPVRTLVAAGCQTVVLTNAAGGVGEGLAAGDLVLIRDHLNLMGDSPL
                     RGPNDDRLGPRFPDMTDLYDSKLRELAQRVATQLFGMPLKTGVYAGLLGPQYETPSEV
                     RMLRGLGADLVGMSTVPEAIVARHMGAKVLGMSCVSNLAAGISPVPLSHDEVTETAAR
                     VRGTFLRLLDGIMAALAHVG"
     gene            13641..14132
                     /locus_tag="PKL17_13515"
     CDS             13641..14132
                     /locus_tag="PKL17_13515"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNK45800.1"
                     /translation="MDLRAKQSGDVLDEATGQPLPPLLLDLAERARQAQALAYAPYSR
                     FPVGAALLTSDGRVFVGANVENASYGLTVCAERTAVFAAVLAGAHRLSAIAICTNLNP
                     PAAPCGMCRQTLAEFSEDADVVLLSPHGTARLTKLSTLLPLAFRPNALIAAAEKDPDG
                     NGK"
     gene            14116..15477
                     /locus_tag="PKL17_13520"
     CDS             14116..15477
                     /locus_tag="PKL17_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK45801.1"
                     /translation="MGTANNWLLRGGTLVTVDETQPVLTGDLLIIDGVIAALGTIHPD
                     DPRLAGVQVRDVTGCVVLPGLVQAHIHLCQTLGRGLADDLPLLRWLKERVLPYEAALD
                     ENDIALAAQVGIAELLLSGTTNLLDMGTVRHHDALCTAVAKTGIRATLGKAMMDHPDT
                     VPANLRETTEASLREADELCQRWHGHGRLRYGYAPRFLVSCSDSLMRGVAERVTQSSR
                     QGPGIHTHASEQRDEVALIRDRTGQDNVAALRALGVGGPRTVLAHCVHVTEKEISHLQ
                     QDGTHVTHCPSSNLKLGSGIAPVARLLAEKVDVALGADGAPCNNRLDGFAELRLMAIL
                     QKGLLGPEVLPAKTALRIATLGGAKALGQDDVVGSLTQGKRADVVVVGPHSPGQLPAH
                     DPVSMLVYATQGSDVKDVFVDGELLVASGELTPRTGLDVERLRAECADRMPKLLRRAG
                     IAC"
BASE COUNT         3074 a         4868 c         4916 g         2699 t
ORIGIN      
        1 ggcacgctgg cggtgccgcc gccgattctg tggacgtgtg agctttcaga aggcggtggc
       61 caactgatct gtcacgacgc gccgatgcaa ggacagacgc tttcgcatgt gctgcgtctt
      121 cccgcgtcgg aaccgctgtg cagtccagag ctttttgctc ggcttgcggc gggggaaacg
      181 gtggcgctga cggtgtcttc caatcggccc agcgtcgtcg aagacatcac cacgccgtgg
      241 cccctgcggc tgacgctgcg caagctggct ttgcccaaac ccgtcgatgg cggcgggctt
      301 cttttgacgg tggccgtcga agacctgagc tttagtcagc aaatccggcg cgaaagtctg
      361 ctcgccaaag agcggctcgc caaagtgatg cgatccgtcg gtgatgcgat cctgacgctc
      421 gatcccaaag cggtgattca gcaggtcaac gacgccagtc agaaagtgct cggtctttcg
      481 cccgacgagc tgtgcggcaa gtcagtgctc gatctggcgg ccaccccgcg cgcccgtcta
      541 cagctcgagg agctgcttcg ccacctgccc cataccgggt ttgccgaggc tgaacttcgg
      601 ctaaaacgtc cgacctcacg caagtcgacc cgttttttgg ggcatgtctc ggcgcttttg
      661 ctcggggacg acagcggtct gcccgccggg gcggtgtttc gcgtgcacga ccaaaccgaa
      721 cgaaagcgcg acgacgccga acgacaagcg ctgcggctgc ggctgctcca gtcggaacgg
      781 ctgtcggcgc tgggtgaaat ggcggcacgg attgcccacg aggtcagaaa cccgctggtg
      841 tcgattgggg ccgccgcgca agtcatcgcc gaggagctgc cgcaagacag tccggttcgg
      901 cccgaagcgg tcgcgattgg caacgaggtg cagcggctcg atcggatttt gcaaaatgtg
      961 ctgcgatttg cccgtccgac ccgcgccagc gtccagcgca ccgacgtggt ggccgtgctg
     1021 cgcgaagtcg tcgatctgct gcgtggcaaa gcccgtggtt tgaccctgcg cgtcgacgtt
     1081 cccgaatcac cgcccggcac cggagtggcc gcacttatcg acggagatca gcttcggcaa
     1141 gtgctatgga acgtgatcat caatgcctgc gaagcatcga cgcccggcga tccgaaccga
     1201 tcgctgattg agtgcacggt acgcaagcag cgcggcggcg ccccgcaaga ccgcccggtg
     1261 cttattaccg tcgctgacag cggtgccgga atcagcccag ccgcccgtcg tcgcgtcttc
     1321 gatccatttt tttcgaccaa gacgcgcggc actgggctcg gacttgcgat aagcaaacag
     1381 atcatcgaag aagccggcgg acggattcgc ctgctcaacc gtcagagcgg cggcacccgc
     1441 gtcgtcatcg agctgcgggt cacgccctat cccgacagca atccgtctag tggccggggc
     1501 cgtccattgg ctcagtgact ttgcgcgcga ccatgaagat gaccgccatc gcaaagtacg
     1561 caccgacgaa cggccacgcc ggctgaagct tcacgacggt gtccgctggg ccaaagccat
     1621 acgagtgaat cagtcccgtc gcactcaccg ctgcaccggc cagacaccac atgctcgcgc
     1681 gctcgaagcg cctttcgatc acttcgacgg tggccgccgc cagcagcatc gacgtaaaga
     1741 taaagccttg ctccagcgca aacgcgcctt cgatgaacgt gtcggacgca tgaaacgccg
     1801 cgtacagctt gtcggaaaac accgggccac cggggctgcc atagccaccc acgcgcagac
     1861 ccgtcttggc caaaagcgaa ccccacgccg ccacacccgg caaaatcccg agcacgactg
     1921 ccggcgcgtg ctctttcggc gtcgcttgga acgactgcgc cgtgatgaca atgccgatcc
     1981 acagcacgat cgccatgccc gcatcgatcg gcaccgccca cgcgatgtaa cccagggttc
     2041 cggtcaggca gaccagcgtc gcaaacacgc catttaggat cgaataaccg gcccgcgccc
     2101 ccagcgcttt ccagccggga tgtccgatgt aaatcgtcgt cggaaagcac gaaccaaaca
     2161 gcgccgccac gatcgcgccc agcccgttta cggccagcga gggtcgcgtt ggataggaat
     2221 cgcccgccgc ttccgccgat tcgacgtttt gcagcgatcc gatcaggtca aacagcgcaa
     2281 tcggcacgat caccggcaaa aaccgccaca aaagtccctg cccgagcgct ggaaacagat
     2341 cgccgaccac cggaatcggc agatgcagcc ccattttcat cactggctgc gccaccgggg
     2401 ccagcttcgt cgcccacgcc agcgccgtac cgattcccac cgcaacgagc ccgcccggca
     2461 gtccgccttt gaacttgacc cgcccaaagt aggtcaaaag cacaatcgac agcgtcgcca
     2521 gaccgacaat cggatgggcg aacgtgcgaa acaaaaatcc cagcgaaata aagccgagcg
     2581 caatgccaga cagcgtcgac aaaagcgcag ctcgtggcgt tgctttgcgc actttttcgg
     2641 caaacagcga cccgagcagc gtgatcaccc ccgaccccag gcacgctgtc agacccgcat
     2701 gccaggccag ccgcgccgga tcgggatgtc ccgccgcctg cgcgctgatc ttcgtcggaa
     2761 gcatcaccaa aaacacgtat gccaaaagcg acaccgtgtt gatgccgtaa ggcagcgcgc
     2821 acacatcggt ccgtcccgtt tccgcagcca gcttcagcgc ttgtcgcgaa tagaagatgt
     2881 tgccaagcag aagcgagacc gccgcgcccg gcaggatgcg accgtagagc agctcgtcgg
     2941 gcagctccag gacgtacttg cagagcgtgt tgatcagcaa aagctgaacc aagttatcga
     3001 gcgcgagacc aaaaaacccg tcaatgtcgc cgcgcaccag ccagcgcata cgtcccccag
     3061 ttaggaaaac cagaccagca taaaccaaag ggaaggcgga aggatagagc gctgttagtc
     3121 gacgatttcg atcgcgccga gcgattcgcc ataccgcagc caagccaggt tgcgcaaaag
     3181 cacgctgcga tcatcggcaa acgagcgaat caggtgggac ggcagccgct tgacggcatc
     3241 ggtcagctcg ggctggctgt caaaaccaag ctgcggagca aaatcgctga cgcgcagcga
     3301 cagtcgcggt agatcgctcg gcgggggcga cggatgtttg gccagtcgcg ggtcgatcgc
     3361 cgccgagatg tccgccgctg ccgccatccg tttcgaggta ttggcaatcc caaagcgagt
     3421 ccgcagcgtc gcgcccaccg acgtgtgatc gtactgcgtc gaatcgacaa agcccttgcg
     3481 caccgtcgga ccaatcagca gcgtcggcac ccgaaaacca acctgggcaa aatcgggatt
     3541 gtcatcgggc agcgtaggcg gcgccacatg gtcataaaag ccaccgtgtt cgtcatacgt
     3601 gatcacaaac agcgtccgcg accactgcgg gctctgaaac acggcggtca gcagcgtcgc
     3661 aatcagcgcc tgcccaagct gcacgttgtg gcttggatga tcgtcgtttg ccaaaaagtc
     3721 gggatcgatg atcgaaaacg gcggcagcgt cccgtttttg cagtctttga aaaactcgtc
     3781 gagcttggcg ctcgggttga tttgcagcat cttgcccgga tacgcgcccc agtaccaagc
     3841 cgccgcgccg gcaatgtagt ttttgccgac gaagcctttg tctttcatgc gttcccacag
     3901 cgtcgtcgga ccgccggtga caaagccggt gttgtctttt ttcccgcccg acgagccagc
     3961 gtgaagataa aaacggttcg gccacgtcgg ccccatcacc gacgcaaacc agcgatcaca
     4021 catcgtgaag cggtccgcga gcgcgtagta gtacggaagc tggctgcgat cgtaaaagcc
     4081 catcacgtcg gggccgtact tttcgccgtg gcgcgcgatg tattctttga cgaagccatc
     4141 gtttttgccg ccgccaaact gggtgtgaca ggaatcccag ccgtgtggcg gatctttggg
     4201 ttcgtagttt aggagcttgg cgacttttat caggttgccc ttgccgtcgg gattgctctc
     4261 gtttcccgaa agaccgcttg ggcggcgact gcccggatag cgcgcgtcgc tctgcatcgt
     4321 cccgaggtaa tggtcaaacg accgattttc catcatcagc acgacgacgg catcgattcc
     4381 ggcgagcagt tctttgggcg tctgcggcaa cagttcgtcc aggtcctttg gcatcgccag
     4441 gtcgggcggc gatgcgtaag cgtcgggccc acccgtttcg tcgccgagcg ggatcatgct
     4501 ttcccccccg acacagccga ccgacagcgc cgacagcgaa acccccaggt gcttgagtgc
     4561 tcgtcgtctg gaaatccctc gtttttcacg actttgggtc atcgtcactc cttgccgaag
     4621 gccggaccat aggtcaagcg atgccgcgcg aacaagggtc gatcgcaaca ccgcccggtg
     4681 ccgtcgcccg catgccccgt cgggcccgtt tgtggctagg ctcgggccca aaagacgcgc
     4741 taagatcgca agccttcggc gtagctccgc tgcgccacaa gggggactcg tggggatcgt
     4801 ttgtgaaagc caagtctgcc aagtgacggt tcatgcccgc ggtgccatcg tgacgcgccg
     4861 cgttgaagta ccgcagacca ccgccctgcc cgacggagac atcgagcttg tcatcccaga
     4921 agtgaccgcc cgctgcgagg ccggcagctt ccgcgcccag cttgatccgg gtcgacgcat
     4981 cgtcctgcaa gtcgaaagcg tgctggagcc gcccaaaagc ccggttcaga ccggcgcaag
     5041 tcaggccaag ctcgacgagc tgaagctcaa aatggaccgg ctgcaagcgg agatcgagcg
     5101 acttggcacc cagcggcagc ggctcgccaa acagtttccc gatccggcgc tgcgcaccac
     5161 gtcgctggtt gaaaaagtcg atgaacggat tgctgacgcc attgcgacgg caggactgct
     5221 cgccgaagtg cagaagcaac tgggcgacaa gcagcaactg ctggaacagc agctcgccga
     5281 tttggcgcag gaaaagtccg cggcagagct tcgccacagc caagccccgt cgagcgaaaa
     5341 acaaggacgg agccagccga cacggcgagt gacggttcgc atgaccggcg ccggacccgt
     5401 cagtggtttg ctgatttcgt atgtggtgcc ggcggcgcgg ttttttccta cctatacgct
     5461 gcggcttctc gacggaggca agcgtgcaag ttggtcgatt gatgcgcacg ttgcgcagat
     5521 cagcggcgaa gactggaatg gcgtgcgact gtcgctatca acggcggatc tgatcttcga
     5581 tgcgcggctg cccgagcttc cgtcgctgcg tttcggtcga gcccagcccc cggcgcgccg
     5641 cggatatcgc ccagctcccg acggactgga acgacttttt gccggttatg atcggggatt
     5701 tggtccggct gcgccgacgc caaccgatgg gatgctcgct gaatcgagcc ttgtgccgtc
     5761 gccccccgat agaaccccgc tcaccgatgc gctgcatttt gacgacagcc tgagcgacga
     5821 accgacgtgg gccacccgcg ccgaaaccgc cgatgccgag ctggccaaaa aaggcgggcc
     5881 accgccgctg gaagtcgccg aatatgccgc gccaccgcca ccacctcgcg gagcgcctct
     5941 gccgccgcca tcggcgatgt ttgccatggc accgccggcc ccacaagccc cggccccgtc
     6001 gcgttttcaa agtgcgcccg cgcgatccgg cggtgcagcc agcttgggcg ccgaacccaa
     6061 gcgcaaacgg atggcgaaag aagcccccgg cggtggaggc ggtggttacg acgacgaggg
     6121 tggcgccggt tttgatagcc aaggtgcggt ccccagcttc gatgaagccg ccccgcccga
     6181 cggaatcgaa ccgcaggatg cctggctcga ttttgatcag cttcagctta gcggccccga
     6241 agatcagatg tttcgcggca agctgaagcg gcgcagcgac gatcaatcgc gcagcaagct
     6301 acagcaagcg gtgcgcacga tcgaaaaact gcaagctccg acgggactta tcgatccgat
     6361 cgtgtcgcgc ggacagtttg accatcgcta cgacgcagaa ggacttatca agattccgtc
     6421 ggacggcaag ctgcaccgcg tcgccatctt gaccgccgag ggaagtccgc atttgcacct
     6481 catcaccgtc ccgcgcgagc gtgccgacgt atatcgcgag gcggagctga aaaacccctg
     6541 cgatgcgccg ctgctcagcg gtcccgtcga ggtcttcgtc gacggcgggc ttttgaccac
     6601 cgattcgata agccacgtcg acaaaggcgg cagcgtccgc gtcggcatgg gctgcgaaga
     6661 ccgggtccgc gtcgcgcgca acgtccgcgc cgaagaggaa agcaccgggc ttctcggtgg
     6721 ctcgacggcg atgacccaca cggtcagcat tgaagtcagc tcgtcgcttg ggcagccgat
     6781 tcagatcgat gtgctcgatc gaatgccggt cagcgaagac aaagggctca ccgtcgaaat
     6841 ggtgtacgaa aaaccgccgc acacgccgta tgaccaggcc gaacgcggcg ctccgattcg
     6901 tcgcggcatg agatggcgga tgccaattcc cgccggtggc aaggtcaaga tcgaatacca
     6961 gtaccgcatc ggcttttcgg ccaagagcga gatcatcgga ggcaaccgcc gtgactaacg
     7021 gaagccagca gaccgaagtg cagcggatcg agcttgatac caagccgctg cgggtgacgc
     7081 tttttgaaga tcgcgccgag gtggtgcgcc aagccaaagc gcagttgccg acgggaacgg
     7141 tgatggtcag cctgcgtggg atcacgccgc tcgtcgacga tcagagcgtg cagccgatgc
     7201 tcagcggaac ggtcaagctt ttgtcatcgc aggtgacacg gcgcagccac gaagctccga
     7261 cgggtagtga aaccgagctt agcgaaagcg aaaccgacca aaaacaagca tcggcgcggc
     7321 gcgtcgcggc ggagcgtgca ctggaagttg cgcaggttca cgaaggccgc acaagtgccc
     7381 tgttgctcgc gtggatcgtt gcgctgggtc gcgtaccgca gggggcagcg acggggctgg
     7441 ctggctggcg cgcggcactg acgcagcttc tttcggcgca gacaggcgcg cttgatcagc
     7501 tgagcgcggc gcaaaaagag atccttgcgg ccaagcagga cgaaaagcgc gcccagcttc
     7561 gttatcaggc ggcgcgcagg ctgcgacccc gacgggaatc gacgattgat ctccaggtcg
     7621 aagtgccgca aacgaccgtc gtgaccgtcg aagtgacgta cattacgccg tgcgcgctgt
     7681 ggcggcccga acacctggcg cggctttcgc aaaaccccga cggaactgct gtgctgcaag
     7741 tggtcacgtc agcggtggtc tggcagcgct gcggcgaaga atggaacggc gtcgtctgtc
     7801 gcttttcgac ggcgcgcccg gcccagcatg catcgccgcc gctgcttagc gaagatcagc
     7861 tccggctgcg acgcaaaacc gacgccgagc gccgcaccat catcgtcgaa gcccgcgaac
     7921 agagcatcgc cgtcgctgga cttggtcgcg gcagccaaaa agtcgaggaa atgcccggcg
     7981 tcgatgacgg tggcgaaccg ctgacttttg aagccagccg tccggttcag attttgtcga
     8041 cgggtcagcc ggtgcggatc gaaatcgccc aagtcaccat gcccgcccag gtcgagcgcg
     8101 tcgtcttccc cgaacgcggc atggcagccc acctgcgcgc caccgccacg ctatcggggc
     8161 ccagaccgct tttggccggt ccggtcgtgc tcttgcgcgg cagtgagctg tgcggacgcg
     8221 gacgcacaaa cttcgtcgga cgaggcgagc cattcgagct gggctttggt cccgacgacg
     8281 gcatccgggt tcgccgccag accagcgagg agcgcgagca agccgcaatc accggcacgc
     8341 agcgcatcgc tcgcaccgtc aagctgttcg tcagcaacct cagcggcaga agccgcaagc
     8401 tgaccatcac cgagcgggtt cccgtaagcg aaatccgcga agtcgaaatc agcgtcgaaa
     8461 aaaccagcgg catgcgctgg gacggcaaag acggctttgc ccacttcgat gtcgaagtcc
     8521 ccggtcgcgc caccaaagag cttttgctga gctaccacat cgaagcgcca agccgagtcc
     8581 agcttccccc cgcatagcga cgacgctcct gcaaaagtga cacaaaacat gtcacccgaa
     8641 gtggcactca tatatcaccc gaagtgacac tcgtatgtca cccaaagtga cacccatcgg
     8701 gaagccgaga cgcgcaggtc agttggcgcg cttttggaag cagcttgggc agccgcagcg
     8761 gctttgcagc gaaacgattt cgtcgctggg caagtcggcc agtcggccta gttcgacggc
     8821 acacatgccc cgctgattga ccgcttcgac gacgcgctgg agcagaagga gccacagctt
     8881 cgcccgacag cgctcgccaa gcgagcctgg cagcggaagc ggtggcgaag cgaggggcgg
     8941 tctcatcgag ggtttttcct tgctggcgga ctcgtcggga agcgcggcgc atcatagcca
     9001 caagcgattt tggcaccaga aaatcttcac ttggccttta cagcgtcggc agatgcgttg
     9061 taaagccagg gccaaggcca caatgtctag tcacgatccg caaatggaac agttggttgc
     9121 cgaagtcgct cgtcgcgtcg cggcccgctt aggtggtagc cagccactgg ttcagcttgg
     9181 tacggcgccg tcgctccgcg aacaaccttg ccccgatggt ccgtcggaaa actgcacggc
     9241 ctgcgggctg tgtacggtgc gtcgcccgga agccgtgcaa aacatcgtca gcgaaggcgc
     9301 aagccgcgtc ggttcgggtc ttgggacggg acagacgccc gacagcatcg cccagatgat
     9361 cgaccacacg ctgctgaaag ccgatgccac agcggcggaa gtcgaaaagc tgtgttccga
     9421 agcacggacg taccgttttg cgtcggtgtg tgtgaactct ggctttgttc cgctcgccaa
     9481 gcggcttttg cgcgggtcgg gcgtgatggt ctgtgccgtc gtcggtttcc cactcggagc
     9541 gatggcacca aacgccaaag cgtttgaagc gcgcgaggct gtccgcgccg gtgccgaaga
     9601 gatcgacatg gtgataaacc agggtgcgct caagtcgcgt gactatgcgc tcgtccacga
     9661 ggacatccag aaagtcgtcg aggcgtcgcg cccggccaag gtcaaagtca tcctcgaaac
     9721 cggcgcgctg aaccaggaag aaaaagtcgt cgcgatttct ctgtccaagg tcgcgggcgc
     9781 ccattttgta aaaacgtcga cggggtttgg tcccggcggc gccaccgtcg aagacatcgc
     9841 actgatgcgc aagctcgtcg gcccggacat gggcgtcaaa gcgtcgggcg gcgtgcgatc
     9901 ctgcgacgat gcccaaaaaa tgaaagcagc cggcgcgagc cggatcggcg ccagcgcgtc
     9961 tgtcgccatc gtcaaaggtt gttcaacgcc cgcgccgagc gaagccccga gcaaatatcc
    10021 aaatcgtcct tggcttgcga accgccaagc caaaccaagc gccgctgcgg ctccgacggg
    10081 aaaaccgtcc gcttactagc gacgcccgct ggctttgcgc agaatctaac aggatatcga
    10141 ccgacgggaa ggacgagaga agatgacgaa cgcggaagcg ccgtttgatc gcgtggtgct
    10201 gattgtgctc gattcgtgtg gctgtggttc gtcgccagat gcagaccgtt acggtgacgc
    10261 gggggcgaac acgctgggtc acatcggcga gcgcgtcggc ggaatccgac tacccaactt
    10321 ggcggcgctg gggcttggca acgtcaccca gattcccggt gtgccgcccg tcgacaaacc
    10381 gctcggtggt tttggccgga tgcgggaaag ttcgcgcggc aaagacacca cgacagggca
    10441 ctgggaactg gctgggctcg acgtgcagag cgagtttccg acctatccaa gtggctttcc
    10501 cgccgacatg attgcgcgct tttcgcaagc gacgggacgg ggcgtgctgg gcaatcggcc
    10561 tgcttcggga accacgattc tcgacgagct ggggcccgaa cacctgcgca ctggatcgct
    10621 cattgtctac acgtcggcgg acagcgtgtt tcaaatcgcc gcgcatgaac aggtcgtgcc
    10681 gctggacgag ctgtatcggg cctgcgaagt cgcgcgcaag ctgtgcgacg aaaaaaacat
    10741 cagccgcgtc atcgcaaggc catttgtcgg cgagccggga cggtttaagc gaacctacaa
    10801 ccgtcgagac tacgccatgc caccgccgca agagacggtg ctgtcgcggc ttgcaagctc
    10861 gggccagcca gtggtcggcg tcggcaaaat ttgggacatc ttcgcgggtc acggcatcac
    10921 cgacaacatc cacagcgaag gaaacaccga cggtctgcgg ctgaccctgg atgcgctttc
    10981 gcagacgaaa cgtggcctga tttttgtcaa cctggtcgac ttcgacatgc tgtatgggca
    11041 ccgtcgcgat ccgcagggtt acgcccgcgc gctggtcgag tttgacgaat ttttgccgtc
    11101 gctgcacagc aagctgacgc cgcgcgactt ggtgcttctg actgccgatc atggcaacga
    11161 tccgacgtgg acgggcaccg atcacacccg cgaagacgtc ccgctgctcg cgtttaccac
    11221 cactgcaccg tcgccgcaga cggctgacat gggcctgcgg tccggctttt tcgacgttgc
    11281 ccagacgctc tgcgatgcct ttgccctgcc ggcttggtcg cgcggcaaaa gcgtgctgcc
    11341 ccaccttcta ccgtcctgag cgcactttct cgtcgctcag cctctttaca aagagtcccg
    11401 atggccacat acaacccggt tgaaatcatc cgcaaaaaac gtgatggaaa cgtcctcagc
    11461 agcgacgaaa tccgtcagtt tgtttccgat tacgcgcaag gcaagctgcc cgactaccaa
    11521 gcggcggcgc tgttgatggc gatttacttt accggcatgt ccgaaggtga gcttctcgcg
    11581 ctcaccgacg cgatgctgcg atcgggcgaa gtgattagcc tgggcgacgt gccggggctc
    11641 aagatcgaca agcactcgac gggaggcatc ggcgacaaaa tctcgctgca cctggccccg
    11701 gcggtggcgg catgtggcgt caaagtgccg atgatttcgg gccgcggact tgggcacacc
    11761 ggcggcacgc tcgacaagct ggagtcgatt cctggttttt cgacgcggct gtcggtacaa
    11821 gagtttcgca ccgtgctgcg tcgggccggg ctgtcgctga ttgggcagac cgatacgctg
    11881 gttcccgccg acaaagcgct gtacgcgctg cgcgacgtga cggcgacggt ggaatcgatc
    11941 ccgctgattg ccgcatcgat catgtcgaaa aagctggccg aaggcattga tgggctggtg
    12001 ctcgacgtga aggtcggttc gggcgcattt atgaagacgc gcgacgcggc gtaccagttg
    12061 gcgtcaacgc tggtggcgat tggtcgacgg gcgggcaagc aagtaacggc gtttttgacc
    12121 aacatggatc agccgctcgg tcgctacgtc ggcaatgccg cggaagtgat cgaaacgctg
    12181 caagtgctcg aaggtggcgg cccggcggat ctgatcgaac taacccgcgt gctcggtggc
    12241 gaaatgctgc ggcttggcgg cgtggtggcg tcggaagcgg acggccaggg gcgcatcctc
    12301 gaagtgctgc aaaacggcga agcgctccag cgactgcagc gctgcgcggc gatgcaaggc
    12361 gcgatgatcg acctgcgtga ccagagcgag ccgtggcggc aaaactgcct gcatccgtat
    12421 ccggtgtacg tggtggcgcc cgaggctggc tacgtttcgt cgattgatgc ggaagcggtg
    12481 ggctgggcca gcgtcgcgat cggtgcaggc cgcgagcgca aagaagacac catcgatccg
    12541 gcggcgtaca tctaccttga tcgcaaagtc ggcgagctgg tcgaagcggg cgagcccctg
    12601 tgtcacttcg cgtgttccgc cgattggccg gaaggtcgac gggaacgggc ccacgcgctg
    12661 ctctctgggg cttacgcgat ttcgatgacc ccgcccacgg aaaagccgct gattttggac
    12721 gtgctgcgat gaccgacaga cattcccaca gctcgcatct agcaacctac gaggccgtgc
    12781 agcagtcagc gacgcggatt cgcaacttca tgcccgcggg agcggcccca aaagtcgcgc
    12841 tggtgctcgg atcgggactg ggcagttatg ccgattcgct gaccgaagcg cacagcatcg
    12901 actaccgcga aatcggtttc ccgagttcta gcgtccacgg ccacgccggc aagctggtgt
    12961 atggccgcgc ggcagcgaat ccgtcgacgg gaaatcgcgg tgtcgaagtg ctgctcatgc
    13021 agggccgcgt gcacacctac gaaggccaca gcatgcagca ggtggtgctg ccggtgcgga
    13081 cgctcgtcgc ggcaggctgt cagacggtgg tgctgaccaa tgcggcaggc ggcgtcggtg
    13141 aagggctggc cgcaggtgat ctggtgttga ttcgcgatca cctaaacctg atgggcgatt
    13201 cgccgctgcg cggtccaaat gacgaccgac tggggccgcg cttccccgac atgaccgatc
    13261 tgtacgacag caagctgcgc gagctggcac aacgagtcgc gacgcagctt tttgggatgc
    13321 cgctgaagac cggtgtgtat gccgggctgc tcgggccgca gtacgaaacg ccgtcggaag
    13381 tccgcatgct gcgcggcctg ggcgctgatc tggtcggcat gtcgacggtg cccgaagcga
    13441 ttgtcgcgcg tcacatgggc gccaaagtcc tgggcatgag ctgcgtgagc aacctggccg
    13501 ccggaatttc gcccgtgccg ctgtcgcatg acgaagtcac cgagacagct gcgcgcgttc
    13561 gcggtacgtt tttaagactg ctcgacggga tcatggcggc actcgcccac gtcggatagt
    13621 ccgcaaggag aacgtactcg atggaccttc gcgccaaaca gagcggtgat gtcctagacg
    13681 aagcgaccgg acagccgctg ccccccctgc ttctggatct ggctgagcgc gcacggcaag
    13741 cccaagcgct ggcctatgcc ccgtattcgc gttttcccgt cggtgcagcg cttttgacga
    13801 gcgatggacg ggtgtttgtc ggggccaacg tcgaaaacgc ttcgtatgga ctgacggtat
    13861 gcgcggaacg aaccgcggtg tttgctgcag tccttgccgg tgcgcatcgg ctttctgcca
    13921 tcgcgatttg taccaacttg aatccgcccg cagcgccgtg tggcatgtgt cgtcagacgc
    13981 tggctgagtt ttccgaagat gcggatgtgg tgctgctgtc gcctcatggc acggcgcgac
    14041 tgaccaagct atcgacgcta ctaccgctcg cattccgtcc gaacgcgcta attgcggctg
    14101 ccgagaaaga ccccgatggg aacggcaaat aactggcttc tgcgcggcgg cacactggtc
    14161 acggtcgatg aaacccagcc agttttaaca ggcgatctgt tgatcatcga tggggtgatt
    14221 gcggcgcttg ggacgattca tcccgacgat ccgcggctcg ctggggtgca ggtacgtgac
    14281 gtgacgggct gcgtcgtgct gcccggactt gtgcaagcgc acattcatct gtgccagacg
    14341 ctcggacgag gtctggccga cgatcttccg cttctgcgct ggctcaaaga acgggtgctg
    14401 ccgtatgaag cagcgctcga cgaaaacgac atcgcgctgg ccgcccaggt cggcattgcc
    14461 gagcttttgc tgtcgggcac caccaacctg ctcgatatgg ggacggtacg gcaccacgac
    14521 gcgctgtgta cggcagtcgc caagacaggg attcgcgcaa cgctcggcaa agcgatgatg
    14581 gatcacccgg acacggtgcc ggccaacctg cgcgaaacga ccgaagcgtc gctgcgcgag
    14641 gctgatgaac tgtgccagcg ctggcacggc cacgggcggc tgcgttatgg ctatgcgccg
    14701 cgttttttgg tgtcgtgtag tgattcgctg atgcgcggcg tggccgagcg agtaacccag
    14761 tcatcccgcc aaggtccggg catccacacc cacgccagcg aacagcgcga tgaagttgcg
    14821 ctgattcgtg accgcaccgg gcaagacaac gtggcggccc tgcgcgcgct gggggtcggc
    14881 ggaccgcgca ccgtgttggc gcactgcgtc catgtcaccg aaaaagagat ttcccatcta
    14941 caacaagacg gcacccatgt gacgcactgc ccatcgagca acctgaagct gggttcgggg
    15001 attgcgcccg tggcgcggct gttggccgaa aaggtcgatg tcgcgctcgg tgcggacggc
    15061 gcgccctgca acaatcggct ggatggtttt gccgagctgc ggctgatggc gattttgcaa
    15121 aaaggactgc tgggtccgga agtgctgcca gcaaaaacgg cgctgcggat tgcgacgctg
    15181 ggtggcgcca aggcgctggg acaagacgat gtcgtcggca gtctgacgca aggcaaacga
    15241 gccgatgtgg tggtggtcgg cccgcacagc cccggacagc tgcctgcgca cgatcctgtt
    15301 tcgatgctgg tctacgcgac ccaaggcagc gatgtcaaag acgtgtttgt agacggagaa
    15361 ctgctggtcg caagcggcga gctgacccca cgcaccggac ttgatgtcga acgtctgcgt
    15421 gccgagtgcg ccgatcggat gccgaagctg cttcgccgcg ccggcatcgc ctgttgaagc
    15481 ggattacttg gactctttgc tgccaatgat ggtgaggttg gcgccgacct gaacctggac
    15541 cggcttgccg tccacca
//
LOCUS       DAORGN010000120        15341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1592_length_15341_cov_5.953160, whole genome shotgun sequence.
ACCESSION   DAORGN010000120 DAORGN010000000
VERSION     DAORGN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_15341_cov_5.953160"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKL17_13525"
     CDS             <1..248
                     /locus_tag="PKL17_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNK45802.1"
                     /translation="IDKQTWNRRYRGFMEKIKTGSLFEVAEVFRDLYRLKNQKTLSFG
                     ERRMLDTARTLIVKELSVAKGTTESKVEREIEKLFSA"
     gene            577..1884
                     /locus_tag="PKL17_13530"
     CDS             577..1884
                     /locus_tag="PKL17_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNK45803.1"
                     /translation="MGALGPWAGCEPQAPTCAQSSCLTLSLHAPPGQSLTAADVIVRD
                     GDTLEKKSFWGAELSAPLVLSAPVGRVDSTRRAIYVRAISEDGGGRHSLTGQFEGTAA
                     SSSVDVALTTHCASCRPSARHGAAMTYEPLTRTVLVHGGMGTAGVPLDDLWAWNGLYW
                     NQLATGPGPVARSGHVMAHDPQTGMVLLAFGQGPNETLDDTWLLDPKTLRWQKVAGVG
                     PDARRHAALSPSTGEANRGLLLFGGQTASGQLLGDTWLWDGAAWQAGPSSLCDSRPLV
                     PGTLPRCRMGATLVPSAAGSRTLLYGGLVAASADNLATYDDSVWQFVDGNWSVATSGM
                     PAFVLGRSSHAAALLFHRGQPSEVWLGLGDSPVGLRQDSYLVQDSKPPQPIIGDAPSP
                     RREAATAFDAEREELVIFGGEGAGGALGDTLTFSAETGFVRRD"
     gene            complement(1888..3501)
                     /locus_tag="PKL17_13535"
     CDS             complement(1888..3501)
                     /locus_tag="PKL17_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HNK45804.1"
                     /translation="MAIRLGPGRWLNAQLQLLSWLGEDLHGESFLAEQRPQGRTVVVK
                     ILHSEACRTPAYIERFRHIAERTMLLSHRHLAAVHAFGVLQPDGERGQASPASAELGL
                     RTVSQSDQGIPWIATDPLEGPDLRSYLADGITPDTLLTLLTPLADALDQAAALGLCHG
                     ELVPQNLLLLEEPEKTPRLVLVDLGVAKVLASDLLELPMPPGSLGYRSPEQCRDGREV
                     TAASDRYALATLLFEAVAGRPPFVASDPHKLLQQHLSAPVPPLRTLVPQLRNAEALDT
                     FFVRALAKQPEARFPSAMAMLEEFHEAVSQRPKRANRDGERRRSPRWYRLIRQGQPDK
                     VIDVVVGPRAVLGKQSECDMVCQALPSPDYDSLTAAISREHAVLIWLDNRLSVLDQSS
                     NGTFVNDKRIGPRLTQLPDGAMLRLGEHLSVQVHLIRGGSRPGVPTLGDNSDAAGVLL
                     IRRDRFATGAPNTLMLWSTIDLNNSPLGRLGGALRLGQAWVSQRELWWSGHPTVVWQR
                     QDRSTVLGPAPLRHGDVLASSDTQITVEH"
     gene            complement(3514..5013)
                     /locus_tag="PKL17_13540"
     CDS             complement(3514..5013)
                     /locus_tag="PKL17_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK45805.1"
                     /translation="MVRRTKGTSSPTKRRLAQAETDDGAEARLAAAQDDLDANGADDE
                     PAVTQREEKAVVAAASTMTPRTAGKRRRGVPITIKRRPAKKKAESEAEKPAPSDEDGE
                     KSAAKSSGADPIGSYFHQITRLPVLSSASEYELARRIGIMEEVLWVQILSLPLLTSHL
                     LDVLTEVLGKAPPELPGLRKVATEVMATEPSTSKRLSQAAGPLAAKLRQLDLDRIYIG
                     LILDEIRRLDNSRKAGRSQHTQAIKDDTWQIYVQGTGVISQLIQRAKEDFVKANLRLV
                     VSIARRFDRGRMPLADLIQEGNMGLIKAVERFDYRRGYRFSTYASWWIRHAIARGVAD
                     KSRVVRLPVHMMADLHTLLRHKKRLQRELGRAPTSEELATATGFKLDKVAKADLSLVE
                     DAVSLDREVSQQDHRSFLELLEDDSAELSVSERLISEGMLREVQHLLKNLTGIEADIV
                     KLRFGLERDHELTFQEIAEKYQLSKERIRQLQERALERLRKAMSRKDLL"
     gene            complement(5092..6165)
                     /locus_tag="PKL17_13545"
     CDS             complement(5092..6165)
                     /locus_tag="PKL17_13545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNK45806.1"
                     /translation="MHSDVDLDDSDDSIVDDDSDSSGHDGKMVSTVVLSDASGRPSII
                     EQRFIVESECHGFRLDRFLQKKIRRLSRARIQRVIHGDCDVEGRCARPNMRVYTGQKV
                     SFRRPAPVEPEVPRNISVVYSDPDFYLIDKPAGLPIHPTARYHLSTLTAVLRERFPDE
                     KLQVCHRLDRETSGLMLVARNPVSSSKLKTAFQKRLIDKRYLAIVHGELDLPDGQELL
                     VDKPLGPAGTLVRVRMAVRDLSDGGLPAQTHVRVLQRFAGYTLVECKPHTGRQHQIRV
                     HLWQIGFPIVGDKLYPDEELFIKWSEEGDAAIQDQVPLPRHALHAAGIAFPHPRSGEI
                     LRIDTDLPADLSQFLNTLPHRPY"
     gene            complement(6169..7434)
                     /locus_tag="PKL17_13550"
     CDS             complement(6169..7434)
                     /locus_tag="PKL17_13550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNK45807.1"
                     /translation="MRHQPQSTSRAPTFTSRSHLPIIELARPLRDAIWLGHPWIYRQA
                     LRSPPDLPSGTVVDITDRNGFVARGLYDQHSPLAVRVYTLDESESVDASLVAKRLSAA
                     YQVRRRTLPGLGTETTGYRLCHGEGDRLPGIVIDIYGPIAVVITDGDAARTLLSAVVS
                     ALKACADELGITTIYERHQRRQGHGGGLLFGNPPQLAPEDRGRHPLELVFREHGMRLL
                     VDVENGQKTGMFLDQRENRRLIRDISAGLSVWNGFSYTGGFSLAAALGGATRVVSVDR
                     AEAAIDTARRNFALAGLPLADHEFLAEDAFAALARSKTRGEHFDLVIVDPPSFAPTQQ
                     AVPGALSAYRRLHEQAIDLVTQGGLLAAASCSSHIDEKAFLSTICDAAVAKKRAVQLL
                     EIRSQPSDHPTLPAFPEGRYLKFILCRVS"
     gene            7556..10186
                     /gene="clpB"
                     /locus_tag="PKL17_13555"
     CDS             7556..10186
                     /gene="clpB"
                     /locus_tag="PKL17_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNK45808.1"
                     /translation="MRVDKFTNKTREALQSAQQLAAEKGQAELLPEHLLWALLVQENG
                     VAPALLGKLAAREGGMLDGSRLVSDLERYIDQQPRAQGALDVGLSRRTRDLLETAEKE
                     ALKIKDEYTSTEHLLLALCAKDFGHASKLLRDAGVSADKLMQALSEVRGGQRVTDQDP
                     EGKYQALERYTRDVTDLARKGKLDPVIGRDEEIRRSIQVLSRRTKNNPVLIGEPGVGK
                     TAIVEGIAQRIVGGDVPESLRNKRILSLDLGSLVAGTKYRGEFEDRLKALLKEMSQAA
                     GQIILFIDELHTLVGAGAAEGAQDAANMLKPALARGELRCLGATTLDEYRKHIEKDKA
                     LERRFQPVFVGEPSVEDTVSILRGIKEKYEVHHGIRIRDAALLAAARLSDRYIADRFL
                     PDKAIDLIDEAAARLKMEIDSMPAPIDKLERRIVSLQIEQQALAKEKDEASKERLDEV
                     ADEIANLREQASVMKVRWQQERDVIAKLRSLAAEMDKNKTETELAQRKGDLNRAAELR
                     YGKLPELERQIAAENKRLAEIQKGGAYLREEVTEEDIASIIAKWTGIPVEKMLEGEME
                     RLVHMEDRLSGRVIGQAAAIAAVSSAVRRARAGLQDPNRPIGSFIFLGPTGVGKTELA
                     RALAEFLFDDETNMVRLDMSEYMEKHTVARLIGAPPGYVGYDEGGQLTEAVRRRPYSV
                     VLFDEIEKAHPDVFNVLLQILDDGRLTDGQGRVVSFRNTVILMTSNLGSQYIQMADDL
                     ESPEVKRNIDAALRGHFKPEFLNRIDDIIVFHRLGREHLGSIVKIQLKRLQKLLADQK
                     LSLELSDAAMDLLCEEGYDPTFGARPLKRAIQRLVQDPLAQKILTGQFKPGDTIHVDR
                     DGDKLSVGQVAFAKTTDSRS"
     gene            10249..11196
                     /locus_tag="PKL17_13560"
     CDS             10249..11196
                     /locus_tag="PKL17_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNK45809.1"
                     /translation="MAQPDHYKTLGVAEGASAEEIKKSYRKLAKRYHPDATGGDKAKE
                     SKFKEITEAYEVLSDEQKRKEYDEVRKNPFASGMPGGGFPGGGFPGGFPGGRSRVRTG
                     GGGIDINLEDILGGGGFGDIFSRMGQDAGAGTRRPREAPRGGDIQTTLTLTLAEAALG
                     AEKSFTLGPSTPDERKLTVRIPAGVEDGETIRLSGQGRPSPIGGQAGNLLIKVTVLPD
                     ADFRRKGHDLERDVRIPIDKAVLGGTVEVPIIDGGIKEIRLPAGTSSGLKLRLRGFGA
                     SDRKGGRGDMYVVVQIDVPKDISAEARSLIERFAQLTRR"
     gene            11263..12720
                     /locus_tag="PKL17_13565"
     CDS             11263..12720
                     /locus_tag="PKL17_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK45810.1"
                     /translation="MTLARILILDDQRAARRVLRQMLEGLDEAEFAEAGTVEQALSLC
                     REQRFDAYLVDIHLSENRLERGGIEFIKQVRNIQAAPSVVVTASVEMQDLREAMKAGA
                     VDYVLKDQLSPELILPILREIFEKRDLRERVQQLGQRLDQSFGLGAIVGTSAKIEHVR
                     ALVRRLADADATVLLRGESGTGKELVAQALHQCGKRASYPLVTVNCAAIAPALIESEL
                     FGHERGAFTTALSRRIGVLEEAGAGTVFLDEIAELTPDLQAKLLRVLEARRFTRVGGN
                     EAISFRARVIAATHQDLEQRIREGKLRADLYYRLNVVTIDVPSLADRREDISVLCNHF
                     VSQFGRPLRFTKEALSSLEQRPWPGNVRELRNAVERLALLCESDVVTETALLKVVPLR
                     SSLPVDSDEVERLARQFLARDMAGENKLDFMERTMVRIAMEVAGHNQSRAARILGLER
                     KALERRFKRHLQETSGEVRMPRSAHSAGDSDEHLP"
     gene            complement(12688..15045)
                     /gene="pbpC"
                     /locus_tag="PKL17_13570"
     CDS             complement(12688..15045)
                     /gene="pbpC"
                     /locus_tag="PKL17_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNK45811.1"
                     /translation="MRLPRLTHLPRLPRLLRWLLAVIAVVLLTGIVLRLVPAKPLSFG
                     LPLSTAVYDKDKRLLRLTLADDEQYRLWVPLSDISPQLVEAVLLHEDRHFYHHFAVNP
                     VALSRALWTTYVSRERRVGGSTISMQVARMKYHLYSRSAGGKLRQIGRALWLELRYSK
                     AEILEAYLNLVPVGKNIQGVGAASFVYFGKPPSKLQLGEALTLAVIPQSPARRAPDRD
                     DSQALQTARAALFQKWVRRHPSAQHDADLIKLKLSLRSPHDLPFRAPHLVDSLLTGKP
                     SGTHIETTIDLPVQSIIERQIHSYIERQKRIGIENAAAMLVDTRDMSVRAVVGSAGYF
                     RESILGQVNGTLAKRSPGSTLKPFIYGLAIDQGVLHPQTMLKDSQLSFGAYSPENFDG
                     KFAGPLSAQEALVRSRNVPALWVASKLSNPTLYEFLKTSGVSRLKSESHYGLALVLGG
                     AEVTMEELAMLYAVLPGGGLLRPLRYQKTDPQTAGVRVLSAEASFVTLEMLKENPRPE
                     DTLLTRSLLGIDRVAWKTGTSYGFHDAWTAGVIGPYVLVVWLGNFNGDSNPAFVGVTA
                     AAPLYFSIIDALRQRLHDMQEPSSTRPPNLKRVAVCAISGQMPGRYCKQTKNTWFVPG
                     RSPIDTCEVHRPVEIDTHTGLRACPGQAGQIRSEVYEFWPSDLLRLFRIAGLPRRTPP
                     PYVPGCNLAQVGQHGMAPKITSPLRGVVYTLRPGLDLHDTIGLTSTTDADVGEVFWFA
                     NDKLLGKSRPSIPLLWRPQAGQYVVRVVDDQGRADSRQVLVRISR"
     gene            complement(15112..>15341)
                     /locus_tag="PKL17_13575"
     CDS             complement(15112..>15341)
                     /locus_tag="PKL17_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45812.1"
                     /translation="ELTKPELVQEYKEIAIVEKASGKIYVGDDARTLLGLPIGARIKV
                     RAGDHAGFAVYVQSTSVNRRLVAGTTLLYRK"
BASE COUNT         2985 a         4417 c         4954 g         2985 t
ORIGIN      
        1 acatcgacaa acagacgtgg aaccgtcgct atcgcggctt catggagaag atcaaaaccg
       61 ggtcgctgtt cgaagtggcc gaagtgtttc gcgatcttta ccgactgaag aatcagaaga
      121 cgctgtcgtt tggtgaacgc cggatgctcg acaccgcgcg gacgctgatt gtgaaggagc
      181 tttcggtcgc caaaggcacc accgagagca aagtcgagcg cgagatcgaa aagctgtttt
      241 ctgcctaacc aaactgggtg gccaagcaac cgtaccgcgg ctggattgcg cggtgcggtt
      301 gttttgtttt gggacaaagg tgcgcgtggg ggtgcggctt gcagattggc agctaagcgg
      361 ctgcacggtc gggcatccgc ccgcgcgtga tgcagggttt ttcgtcgctg cgtgttctct
      421 caagtggagg aattgtagat tttttagtct ttgtagaatt gttaaataga acacgttttt
      481 atttgatgat tgagaggttt gcatcaataa tccggggtcg tgactaccca aaatcacagc
      541 tcttttagga aatggggatt acgttttgct ttgcttgtgg gcgcactcgg cccgtgggct
      601 ggttgtgaac cgcaggcgcc gacgtgtgct caaagcagct gcctcacgct gtcgctgcat
      661 gcaccgcccg gtcagtcgct cacggcggcg gatgtgatcg ttcgcgatgg cgacacgctg
      721 gaaaaaaagt cgttttgggg ggcagagctc tcggctccgc ttgtgctttc tgcaccggtt
      781 ggtcgcgtcg attcgactcg gcgagcgatt tacgtgcggg cgatttctga agacggcggc
      841 ggtcggcact cgctcaccgg acagtttgaa ggcacagcgg caagctcgtc tgtagacgtt
      901 gcgctgacca cccattgcgc atcatgccgt ccgtcggcgc gccacggggc tgcgatgacg
      961 tatgaaccgc tgacgcgaac ggtgctggtg catggcggaa tggggactgc gggcgtgccg
     1021 cttgatgacc tgtgggcgtg gaatgggctt tactggaacc agttggcgac ggggccgggg
     1081 ccggtggcgc gcagcgggca tgtgatggct cacgatccac agacgggaat ggtcctgctg
     1141 gcgtttggac aaggtccaaa tgaaaccttg gacgatacct ggctgctgga tcccaagacg
     1201 ctgcgctggc aaaaagtcgc tggcgttggc ccagacgctc gacgacatgc ggcactttct
     1261 ccatcgacag gcgaggcgaa tcggggcctg cttttgtttg gtggtcagac ggcgagtggt
     1321 cagctccttg gcgatacctg gctgtgggat ggggcggcgt ggcaagcagg tccgtcgtcg
     1381 ctgtgtgaca gcaggccgct tgtgccgggg acactgccgc gctgccgaat gggtgcgacg
     1441 ctggtgccat ctgcagcggg gtctaggacg ctgctttatg gcgggctcgt tgctgcatcg
     1501 gcggacaact tggcaacgta tgatgattcg gtctggcagt ttgtcgacgg aaactggtcg
     1561 gttgcgactt ctggcatgcc ggcttttgta ctgggtcgct cgtcgcatgc tgccgcgctc
     1621 ttgtttcatc gcggccagcc gagcgaggtg tggctgggac tcggcgacag tccggtgggg
     1681 ctgcgtcagg acagctacct tgtgcaagat tccaagccgc cgcagccgat catcggggat
     1741 gcgccttctc cccgtcgaga agctgccacc gcctttgatg ccgagcgcga ggagcttgtg
     1801 atctttggcg gcgaaggggc cggtggcgcg ctgggggaca cccttacatt ttctgctgaa
     1861 actggcttcg tccgtcgcga ctagcgctta gtgttcgacg gtgatttggg tatcgctgct
     1921 ggccagcacg tcgccatgac gaagcggcgc cggcccaagc accgtcgagc gatcttgccg
     1981 ctgccacacc accgtcggat gtccgctcca ccacagctcg cgctgggaca cccaggcttg
     2041 cccaagccgc agcgctccgc ccagtcgccc aagcggtgag ttgttcagat cgatcgtcga
     2101 ccacagcatc agcgtatttg gcgccccggt ggcaaagcga tcacgacgga tgagcagcac
     2161 tccagcggcg tcactgttat cacccagcgt cggcacaccc ggtcgcgatc cgccacgaat
     2221 caggtgcact tgcacgctca gatgttcgcc caagcgaagc atcgcaccgt cgggaagttg
     2281 ggtaagccgc ggcccaatcc gcttgtcgtt gacgaacgtg ccgttgctgc tttgatcaag
     2341 cacgctcagt cggttgtcga gccagatcag gacggcgtgc tctcggctga tcgccgcggt
     2401 cagcgagtca taatcgggcg acggcagcgc ttgacagacc atgtcgcact ccgactgttt
     2461 gcccagtacc gcgcgcggtc cgacgacgac atcgatcact ttgtcgggct gtccctgtcg
     2521 gattaagcga taccagcgcg gcgatcgtcg gcgttctccg tcgcgatttg cacgtttggg
     2581 tctttggctg accgcttcgt gaaactcttc gagcatcgcc atcgccgacg gaaaacgcgc
     2641 ttccggctgc ttggcgagcg cccgcacaaa aaaagtatca agtgcttcag cgttgcgaag
     2701 ctgcggcacc agcgtgcgca ggggcggaac tggcgctgac agatgctgct gcaacagctt
     2761 gtgcggatcg ctggcgacaa acggcgggcg tccggccacc gcctcgaaca acagcgtcgc
     2821 cagggcatag cgatcggatg cggcggtcac ttcacggcca tcgcgacact gttcggggct
     2881 gcggtagccg agactgcccg gcggcatcgg cagttccaga agatccgacg ccagcacttt
     2941 ggccaccccc agatcgacca gcaccagccg cggcgttttt tcgggttctt cgagcagtag
     3001 caggttttgg ggcaccagct cgccgtggca aagtccgagc gccgccgcct gatccagcgc
     3061 atcggcaagt ggcgttagca gcgtcagcag cgtatcgggc gtgatcccgt cggccaaata
     3121 gctgcgcaga tcgggccctt cgagcggatc ggtcgcaatc cagggaatcc cttggtcgct
     3181 ctgcgacacg gtgcgcaggc ccagctcggc actggctgga ctggcttgcc cgcgctcgcc
     3241 gtcgggctga agcacgccaa acgcatgcac cgccgccaga tgacgatggc tcagcagcat
     3301 ggttcgctct gcgatgtgac ggaaacgttc gatgtaggcc ggggtccggc acgcttccga
     3361 atgcagaatc ttgaccacga ccgtgcggcc ttgcggtcgc tgttcggcca aaaagctctc
     3421 accgtgcagg tcttcgccca gccacgaaag cagctggagc tgcgcattca gccaccgtcc
     3481 cggtccgagc cgtatcgcca tcagtgcctc aaatcacagc aggtctttgc gcgacatcgc
     3541 tttgcggaga cgttcgagcg cgcgctcctg gagctggcga atgcgctcct tggatagctg
     3601 gtacttttcg gcgatctcct gaaaggtcag ttcgtgatcg cgttcgaggc caaaccgcag
     3661 cttcacaatg tccgcttcga tgccggtgag gttttttagc agatgttgaa cctcgcgcag
     3721 catcccttcg cttatcagtc gttcgcttac gctaagttcc gcgctgtcgt cctcgagcag
     3781 ttcgagaaac gaccgatgat cttgctggga gacctcgcga tcgagcgaca ccgcatcttc
     3841 aacgagcgaa agatcggctt tggctacctt atcgagctta aaacccgtcg ctgttgccag
     3901 ttcttcgctg gtcggcgccc ggcctagttc acgctggagc cgttttttgt ggcgcagcag
     3961 ggtgtgcagg tcggccatca tgtgcaccgg cagccgcacc acccgtgatt tgtcagcgac
     4021 gccgcgcgca atcgcgtgcc gaatccacca cgaggcatag gtcgaaaatc gataaccgcg
     4081 acggtaatcg aagcgctcga ccgctttgat aagacccatg ttgccttctt ggatcaggtc
     4141 agcgagcggc atccgtccgc ggtcaaagcg gcgcgcgatg gacaccacca gacggagatt
     4201 cgctttcacg aagtcttctt tggcgcgctg gatcagctgg cttatgacgc cggtgccttg
     4261 aacgtagatt tgccaggtgt cgtccttgat ggcttgggtg tgctggctgc gtccggcttt
     4321 gcgactgttg tcgagccggc gaatctcgtc gaggatgagc ccgatgtaga tccgatcgag
     4381 gtcgagctgt cggagcttgg cggcaagcgg cccggcggct tgggacagtc gtttgctcgt
     4441 gctgggttcc gtcgccatca cctcggtggc cactttgcgc agaccgggta gctccggcgg
     4501 ggctttgccc agcacttcgg tcagcacatc gagcaggtgc gaagtcagca acggcaaaga
     4561 caggatctgg acccacagca cctcttccat gatgccgatg cgccgcgcca gctcgtactc
     4621 ggatgccgac gaaagaaccg gcaagcgagt gatctggtga aagtagctgc cgatcggatc
     4681 ggctcctgac gatttggcgg cgctcttttc tccatcttcg tcgctgggcg ctggtttttc
     4741 ggcttcgctt tcggctttct ttttggccgg acgccgcttg atcgtgatcg ggacgccgcg
     4801 acgacgtttt ccagcggttc gtggtgtcat cgtcgatgca gctgctacga ccgctttttc
     4861 ttctcgctgt gtgacggcgg gttcatcgtc ggcgccgttg gcgtcgagat catcctgtgc
     4921 cgcagcgagt ctggcctctg ctccgtcgtc cgtctctgct tgagccaaac gtcgtttcgt
     4981 cggtgaagac gtgcccttcg tgcgccttac catgacttgc acaccccaac ctgtgcatgc
     5041 ctcagattaa ggcaaagtgg cagattacat cgaagttctg cgcgcgcagc gtcagtacgg
     5101 tcggtgtggc agcgtattta gaaactgcga caagtcggca ggcaggtcgg tatctatgcg
     5161 caaaatctca ccgctgcgcg ggtggggaaa cgcaatcccg gcggcgtgca gcgcgtgacg
     5221 aggcagcggc acttgatctt ggatcgcggc gtcgccttct tccgaccact tgatgaacag
     5281 ttcttcgtcg ggatagagct tgtcaccgac gatcgggaag ccgatttgcc acaggtgaac
     5341 gcgaatttgg tgctgccgtc cagtgtgcgg tttgcattcg acgagcgtgt agccggcaaa
     5401 gcgctggagc acgcggacgt gggtttgggc cggcaagccc ccgtcggaca gatcgcggac
     5461 cgccatgcgc acccgcacca gcgttcctgc cggcccgagt ggtttgtcga cgagcagctc
     5521 ttggccgtct ggcagatcga gttcaccgtg aacgatggcc aaatagcgct tgtcgatcag
     5581 tcgcttttga aacgccgtct tcagcttcga cgacgaaacg ggattgcgcg ccaccagcat
     5641 aagccctgat gtctcgcggt cgagtcggtg acaaacctgc agtttttcgt cgggaaagcg
     5701 ttcccgaagc accgcggtca gcgtcgacaa atgataacgc gccgtcggat ggatcggcag
     5761 accggcgggc ttgtcgatca ggtaaaaatc cgggtcgctg tagacgacag agatattgcg
     5821 tggaacctct ggctcgacgg gggcagggcg gcgaaagctg actttttggc cggtgtagac
     5881 gcgcatgttg gggcgcgcgc agcggccttc gacgtcgcag tcaccgtgga tgacgcgctg
     5941 aatgcgggcg cgcgacaggc gacggatttt tttctgcaag aagcgatcga gtcgaaaacc
     6001 gtgacattca gattcgacga tgaagcgctg ttcgatgatg ctgggtcgcc cgcttgcgtc
     6061 tgacaacacg acggtgctga ccattttgcc gtcgtggcca ctgctgtcgc tgtcatcgtc
     6121 aacgatcgaa tcgtcgctgt catccaaatc cacgtcgctg tgcatgggtt agctcacgcg
     6181 gcagaggatg aatttgagat aacggccttc ggggaaggcg ggcagcgtcg ggtgatcgct
     6241 tggctggctg cgaatttcca gaagctggac cgcgcgtttt ttggcgaccg cagcgtcgca
     6301 gatcgtcgaa agaaaggctt tttcatcgat gtggctgctg caggatgccg ccgccaaaag
     6361 cccgccctgc gtgaccagat cgatcgcttg ttcgtgcagt cgtcgataag cagaaagtgc
     6421 gccggggacc gcttgctgcg tcggggcaaa gcttggcgga tcgacaatca caaggtcaaa
     6481 gtgttcgccg cgggttttcg atcgggccag cgcggcaaag gcgtcctcgg cgagaaactc
     6541 gtggtctgca agcggtaggc cagcaagcgc aaagttgcgg cgcgcggtgt cgatggcggc
     6601 ttcggctcgg tcgacggaaa ccacgcgcgt tgccccaccg agcgccgccg cgagagaaaa
     6661 accgcccgtg tacgaaaagc cgttccacac cgacagaccc gcggaaatat cgcgaatcag
     6721 acggcgattt tcgcgctgat cgagaaacat gccagtcttt tgtccatttt ccacgtcgac
     6781 gagcagccgc atgccgtgtt cgcgaaaaac cagctcaagt ggatggcgac cgcgatcttc
     6841 tggcgcaagc tgcggtgggt tgccaaaaag cagtccgcca ccgtgacctt gccgtcgctg
     6901 atggcgttca tagatcgtcg tgatacccag ctcatcggcg caggctttga gcgctgaaac
     6961 caccgcggaa agcaaggtgc gcgcggcgtc tccgtcggtg ataaccaccg caatcgggcc
     7021 gtagatgtcg atgacgatgc cgggcagccg atcgccttct ccatgacaga gcctgtagcc
     7081 ggtcgtttcg gtgccaagtc cgggcagggt gcggcggcgg acttgatagg cggcagaaag
     7141 tcgtttggcg acgagcgacg catcgaccga ttcgctttca tccagcgtgt agacccgcac
     7201 cgccagcggc gagtgctggt cgtaaagacc gcgcgcgaca aagccattgc gatcggtaat
     7261 gtcgaccacg gttcccgacg gcaagtccgg cggagaccgg agcgcttggc gataaatcca
     7321 ggggtggcca agccaaattg catcccgcaa aggacgggca agctcgatga tgggcaggtg
     7381 cgagcgcgac gtaaaagtcg gtgcgcgcga ggtggactga ggctgatgtc gcatcgggga
     7441 ccagacttcg taagtccgcg tcgcaaaaaa cgcaagcgct tgcgatgatc agggatgtgc
     7501 ttatgaatgc tttggtcagc gcaaaagtgc cgacacctaa ggagcaaagc ccgtcatgcg
     7561 cgtcgataag ttcaccaaca agacccgcga agcccttcag tccgctcagc agcttgccgc
     7621 cgaaaaaggc caggcagagc ttcttcccga gcatcttttg tgggcgctgc tcgtgcagga
     7681 aaacggtgta gcgcccgcgc tccttggcaa attggcggcg cgtgaaggtg gaatgctcga
     7741 cggaagtcgc ctggtttccg atctggagcg ctacatcgac cagcagccgc gggcgcaagg
     7801 ggcgctggat gtggggctgt cgcgacggac gcgcgatctg ctggaaacgg cggaaaaaga
     7861 agcgctgaaa ataaaagatg agtacacgtc gacggagcac ttgctgctgg ctctgtgcgc
     7921 caaggacttt ggtcatgcca gcaagctttt gcgcgacgct ggcgtgagcg cggacaagct
     7981 aatgcaggcg ctttcggaag tgcgcggtgg ccagcgggtg acggaccaag atcccgaggg
     8041 caaataccaa gcgctggagc gctacacccg cgatgtgacc gatctggcgc gcaagggcaa
     8101 gctggacccg gtgatcggtc gcgacgaaga aattcgccgg tcgattcagg tgctgtcgcg
     8161 gcggacgaaa aacaacccgg tcttgatcgg cgagccgggt gtcggcaaaa ccgcgattgt
     8221 cgaaggcatc gcgcagcgga tcgtcggtgg ggacgtgccg gaaagtctgc gcaacaagcg
     8281 gattttgtcg ctcgacctgg gctcgctcgt cgcgggaacc aagtatcgcg gtgagtttga
     8341 agatcggctc aaggcgctgc tcaaagaaat gagccaggcg gcggggcaga tcatcctgtt
     8401 tatcgacgag cttcacacgc tggtcggcgc gggcgcagcg gaaggagcgc aagacgcggc
     8461 caacatgcta aagccggcgc tggcgcgtgg tgagctgcgc tgcctgggcg cgacgacgct
     8521 cgacgagtat cgcaagcaca tcgaaaagga caaggcgctg gagcggcgct tccagcctgt
     8581 gttcgtcggt gaaccgtctg tggaagacac ggtgtcgatt ctgcgcggca tcaaggaaaa
     8641 gtacgaagtc catcacggca ttcggattcg cgacgcggcg cttctggctg cggcgcggct
     8701 ttcggatcgc tacatcgcgg atcgcttcct gcccgacaag gcgatcgacc ttatcgacga
     8761 ggcagcagcg cggctcaaga tggaaatcga ttcgatgccg gcgccgatcg acaagctgga
     8821 acgacgcatc gtcagcttgc aaatcgaaca gcaggcgctg gcgaaagaaa aagacgaagc
     8881 atccaaagag cggctcgacg aagtggcgga cgagatcgcc aacctgcgcg agcaagcatc
     8941 ggtgatgaaa gttcgctggc agcaagaacg agatgtcatc gccaagctgc ggagtcttgc
     9001 ggcggagatg gacaaaaaca agaccgagac ggagctggcg cagcgcaaag gcgatctgaa
     9061 ccgcgccgcc gagctgcgat acggcaagct gcccgagctt gagcgtcaga ttgccgctga
     9121 gaacaagcgg ctcgccgaga ttcagaaggg tggggcgtat ctgcgcgagg aagtcaccga
     9181 ggaagacatc gcgagcatca tcgccaaatg gaccggcatt cccgtcgaaa aaatgctcga
     9241 aggcgagatg gaacggctgg tccacatgga agatcggctg tcgggacggg tgattggtca
     9301 ggcggctgcg atcgcggcgg tgtcgagtgc ggtgcgacgg gcgcgggcag gactccaaga
     9361 tccaaatcga ccgattggct cgtttatttt cctgggaccg acgggcgtgg gcaaaaccga
     9421 gctggcgcgc gcgctggcag agtttctgtt cgacgacgaa accaacatgg tgcggctcga
     9481 catgagcgag tacatggaaa agcacaccgt ggcgcggctt atcggggcgc ctccgggtta
     9541 tgtcggttac gacgaaggcg gacagcttac cgaggctgtg cggcggcggc cctacagcgt
     9601 ggtgctgttc gatgagatcg aaaaagcgca tcccgatgtg ttcaacgtgc tgctccagat
     9661 tctggatgat ggccgactga ccgacgggca aggccgggtg gtgagcttcc gcaacacggt
     9721 gattttgatg acgtcgaacc tggggtcgca gtacatccag atggccgacg atctggaatc
     9781 gccggaagtg aagcgcaaca tcgacgcggc gctgcgtggg cactttaagc ccgagttcct
     9841 caaccgcatc gacgacatca ttgtgttcca tcgtctggga cgcgaacacc tgggctcgat
     9901 cgtcaagatt cagctcaaac ggctgcaaaa gctgctcgcg gatcagaagc tgtctttgga
     9961 actgtcggat gccgcgatgg atcttttgtg cgaagaaggc tacgacccaa cattcggtgc
    10021 gcggcctctg aaacgagcaa tccagcgcct ggtccaagat ccgctggcgc agaagattct
    10081 gacgggacag tttaagcccg gtgatacgat ccacgtcgac cgcgacggag acaagctcag
    10141 cgtggggcag gttgcttttg cgaagacgac ggacagtcgg tcgtaacgtc ggtcgaaact
    10201 tgggcgcgca ctgcttgcga gtgataagag atttcgggga acacgacgat ggctcagccg
    10261 gaccactaca aaaccctcgg tgttgctgag ggcgcaagcg ccgaagagat caaaaaatcc
    10321 tatcggaagc tggccaaaag ataccatccc gacgcgacgg gcggggacaa ggccaaagag
    10381 tccaagttca aggaaatcac cgaagcgtat gaggtgcttt ccgacgaaca aaagcgcaaa
    10441 gaatacgatg aagtgcgcaa aaacccgttc gcgtcgggca tgcctggcgg tgggtttccc
    10501 ggcggtggct tccccggcgg tttccccggc ggtcgcagcc gggttcgcac cggcggcggt
    10561 ggcatcgaca tcaacctcga ggacatcctc ggcggcggcg gtttcggcga catcttcagc
    10621 cgaatgggcc aagatgccgg tgctgggact cggcgtccac gcgaggcacc acgcggcggc
    10681 gacattcaga caacgctgac gctgacactg gccgaagcgg cgcttggcgc ggaaaagagc
    10741 tttacgcttg gacccagtac gcccgacgag cgcaagctga cggtgcggat tcctgccggc
    10801 gtcgaagatg gcgagacgat tcggctgtca ggacaagggc gaccaagtcc catcggcggt
    10861 caagcgggca acttgcttat caaagtgacg gtgctgcccg acgctgattt tcgtcgcaaa
    10921 ggtcacgatc tggaacgcga cgtgcggatt ccgatcgaca aagcggtgct cggcggaacc
    10981 gtggaagttc cgatcatcga cggtggcata aaagaaatcc ggctgccggc ggggaccagc
    11041 tcagggctca agctgcggct gcgtggcttt ggtgcatcgg atcgcaaagg tggtcggggt
    11101 gacatgtatg tcgtggtcca gatcgacgtg cccaaagaca tatccgccga ggctcgcagc
    11161 ctcatcgaac gctttgccca gcttacccgt cggtaatcct tatcaaattg acacaagcga
    11221 gcgctttcgt cggtcccgct gactgttgta cgatcggggc ccatgacttt ggcccgcatc
    11281 ctgatcctag atgaccagcg cgcggcacga cgagtgctcc ggcagatgct ggaggggctc
    11341 gatgaggcgg agtttgccga agcggggacc gtcgagcagg cgctttcact gtgtcgcgaa
    11401 cagcgctttg atgcctatct ggtcgacatc cacctaagcg aaaaccgcct cgagcgaggt
    11461 gggattgagt tcatcaaaca ggtgcgcaac atccaagccg caccttcggt tgtcgtcacc
    11521 gcttccgtcg agatgcaaga cctgcgggaa gcgatgaaag caggggcggt ggactatgtc
    11581 ctaaaagacc agctctcgcc ggagctgatc ttgccgattc tgcgcgagat ttttgaaaag
    11641 cgcgacctgc gtgagcgggt gcagcagctc gggcagcggc tcgatcagag ctttggactg
    11701 ggtgcgattg ttggtacatc ggccaagatt gagcacgtcc gtgcgctggt gcggcggctg
    11761 gccgatgcag acgcgacggt gctgctgcgc ggcgaaagcg gcaccggcaa agagctggtg
    11821 gcgcaggccc tgcatcagtg tggcaagcgc gcaagctatc cgctggtgac ggtaaactgc
    11881 gcggcgatcg cgccggcgct tatcgaatca gagctgtttg gccacgagcg tggcgcgttt
    11941 accacggcac tttcgcggcg gattggcgtg ctggaagaag cgggcgcggg gacggtgttt
    12001 ctcgatgaaa ttgccgagct tacgcccgac cttcaggcca agctgttgcg cgtcttggaa
    12061 gcgcgacggt ttacccgcgt cggtggcaac gaagcgatct cgtttcgcgc cagggtcatc
    12121 gctgcgacgc accaggatct ggaacagcgg attcgcgaag gcaagctgcg ggccgacctg
    12181 tactatcggc tcaatgtcgt gaccattgat gtgccgtcgc tggctgaccg gcgcgaagac
    12241 atctcggtgc tgtgcaatca cttcgtgtcg cagttcggtc gtccgctgcg ctttaccaaa
    12301 gaagcgctgt cgtctttgga acagcgtccg tggccgggca acgtccgcga gctgcgcaat
    12361 gcggtcgaac ggctggcgct gctgtgtgaa tccgacgtgg tgacagagac cgcgctgctg
    12421 aaagtggtgc cgctgcgttc gtctttgccg gtcgattccg acgaagtcga gcgactggcg
    12481 cgacagtttt tggcgcgcga catggcgggc gaaaacaagc tggatttcat ggaacggacg
    12541 atggtgcgca tcgcaatgga agtggctggg cacaatcagt cgcgggcggc gcgcatcttg
    12601 gggctcgaac gcaaagcgct ggaacgtcgc ttcaagcggc acctgcagga aaccagcggg
    12661 gaagtgcgga tgccgcggtc ggcgcactca gcgggagatt cggacgagca cctgccgtga
    12721 atcggcgcgg ccctgatcgt cgacgactcg aaccacgtac tgcccggcct gcggacgcca
    12781 caaaagcgga atgcttggac ggcttttgcc gagcagtttg tcgtttgcaa accagaacac
    12841 ttcgccgaca tccgcatcgg tggttgaagt aagtccaatc gtatcgtgca ggtccagtcc
    12901 cggtcgcagg gtgtagacca cgcctcgtag cggcgacgta attttcgggg ccatgccgtg
    12961 ctgtccgacc tgtgccagat tgcagcccgg cacatacggc ggcggtgtcc tgcggggcag
    13021 tccggcaatg cgaaacagcc gcagcaagtc cgacggccag aactcgtaga cttcgctgcg
    13081 aatctgtccg gcctgtccgg ggcaagcgcg cagaccggtg tgcgtgtcga tttcgactgg
    13141 gcgatggact tcgcaggtgt cgatgggtga gcgaccggga acaaaccagg tgtttttggt
    13201 ctgtttgcag tagcgccccg gcatttggcc cgagatggcg cagaccgcaa cccgcttgag
    13261 gttcggaggc cgcgtcgacg atggttcctg catgtcgtgg agccgctgac gcagcgcatc
    13321 gatgatcgaa aaataaagtg gagccgccgc ggtgactccg acgaaagctg ggttgctatc
    13381 gccgttgaag ttgccaagcc acaccaccag cacatacggc ccaatcacgc ccgcggtcca
    13441 cgcatcgtga aagccgtagg acgtgccggt cttccacgcg acccggtcga tgcccaaaag
    13501 cgatcgcgtc agcagcgtgt cttctggccg cggattttcc tttagcattt cgagcgtgac
    13561 aaagctcgct tcggcgctga gcacccgcac acccgccgtc tgtggatcgg ttttctgata
    13621 gcgcagtggc cgcaaaagtc cgccgcccgg cagcaccgca tagagcatcg ccagctcttc
    13681 catcgtgact tccgcgccac cgagcacgag cgccagaccg tagtggcttt cgctcttgag
    13741 ccgcgacacg cctgatgtct tcaggaactc gtacagcgtc ggattgctga gcttgctcgc
    13801 gacccacagc gccggcacat tgcgcgaacg aaccagcgct tcttgcgccg acagcggacc
    13861 cgcaaacttg ccgtcgaagt tttccggact gtaggcgcca aacgaaagct ggctgtcttt
    13921 tagcatcgtc tgcggatgca gcacgccttg gtcaatcgcc agcccataaa taaacggctt
    13981 cagcgtcgat cccggcgatc gtttggccag cgtgccgttt acctgaccca agatgctttc
    14041 gcgaaaataa cccgcgctgc cgacgaccgc tcgcaccgac atatcgcgcg tatcgaccag
    14101 catggcggcg gcgttttcga tgccgatgcg cttctgccgt tcgatgtagc tgtgaatttg
    14161 acgttcgatg atgctctgca ccggcagatc gatcgtggtt tcgatgtgcg tgccactggg
    14221 cttgccggtc aggagcgaat cgaccaagtg cggcgcgcga aacggcagat catgcggact
    14281 gcgcaaagac agcttcagct tgatcaaatc ggcgtcgtgc tgcgcgctcg gatggcgtcg
    14341 cacccacttt tgaaacaggg ccgcgcgtgc cgtctgtagc gcttggctgt cgtctctgtc
    14401 tggggcccgc cgcgccggac tctgcggaat caccgccagc gtcagcgctt cgccgagctg
    14461 gagcttgctt ggcggtttgc cgaaatagac aaagctggcg gcaccgaccc cttggatgtt
    14521 ttttccgacg gggaccaggt tcaaataggc ttccagaatt tcggctttgc tgtaacgcag
    14581 ctccagccac agcgctcgtc cgatttgccg tagcttgccg ccggcactgc gcgaatacag
    14641 gtgatatttc atccgcgcca cctgcatcga aatcgtcgaa ccaccgacgc gacgttctcg
    14701 gctgacatac gtggtccaca gcgcccgcga aagcgccacc gggtttaccg caaagtggtg
    14761 ataaaagtgc cggtcttcgt gcagcagcac cgcttcgaca agctgcggcg agatgtcaga
    14821 cagcggcacc cacagccgat actgttcatc gtcggccagc gtcagccgca acagtcgctt
    14881 gtctttgtca taaaccgccg tcgacagcgg caggccaaac gacagcggtt ttgcgggcac
    14941 aagccgcaag acaattcccg tcagcagcac cacggcgatg acagccaaaa gccagcgcag
    15001 cagacgcggc aagcggggca gatgggtcag acggggcagg cgcactgcct catggtagcg
    15061 cgagcttgca caaggcgagt ggctgatggc gggtggtttg gtcggtttcg cttacttgcg
    15121 atatagcagc gtcgtgcccg cgacgagccg gcgattcacc gacgtcgact gcacatagac
    15181 cgcaaagccc gcgtggtcac cggcgcggac tttgatgcgg gccccaatcg gcagtccgag
    15241 cagtgtccgt gcatcgtcgc ccacgtagat ttttccgctg gctttctcga cgatggcgat
    15301 ctctttgtat tcctgcacca gctcgggctt ggtcagctcg t
//
LOCUS       DAORGN010000121        15300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1596_length_15300_cov_5.856740, whole genome shotgun sequence.
ACCESSION   DAORGN010000121 DAORGN010000000
VERSION     DAORGN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15300
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_15300_cov_5.856740"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2128)
                     /locus_tag="PKL17_13580"
     CDS             complement(200..2128)
                     /locus_tag="PKL17_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK45813.1"
                     /translation="MKTTAKTICNRDCPDACSIVATVEDGRITHLAGDPDHPVTQGFL
                     CYRTSHFLETQYSSQRLTKPLLRSSASGKLLPVSWEEALSYTADKLLQIRKESGPASI
                     FYYRSGGTLGMMGAVVDRFWEHFGPVTVKRGDICSGAGDAAQIEDFGIEESSDFFDLQ
                     NAKSILLWGKNPFISSPHLLPLLKDCRRAGAQLALIDPVAHKSASLCQDFVQVRPGGD
                     FALAMAVGQILFAQNWIDPQADSYCDKIREFRALAHSRSLEDWLAEADVSRESAQLLA
                     KRLGQDKPATILIGWGMGRRRNGAAVVRALDALAALTGNLGIPGGGASFYFKRRGAFD
                     SSFVEGRAPRTVCEPLFGREVQNMSDPPIRAVWVTAGNPVAMLPDSQTTADALRSRDL
                     LVVCDHLLTDTAELAHVVFPTTTLLESDDLLGSYGHHYLGVSRPVSPPPPDVKSDLEI
                     IQALAARVGLADVVAGSPRDWKAKMIAPKLGPHGITVDTLEAGPLRNPLAEKVLFADR
                     KFATPSGKVNLIDSLPPSPAKPSAEYPLLLMALSTPDSQSSQWVRPQQGLAELTVHPD
                     SANGIPHGGTGVLASVIGKMQVRVLHDPAQRKDVAIVPKGGHLRDGRCANALTEAALT
                     DLGEGGALYDQPVKLTPL"
     gene            complement(2125..4215)
                     /locus_tag="PKL17_13585"
     CDS             complement(2125..4215)
                     /locus_tag="PKL17_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK45814.1"
                     /translation="MAKKLALIYPPTCDPTAPYLAVPMLTGFLRSHGREVLPIDANIE
                     AWDGLLSLDRLTDVAHRLETQLAELEKKPQLTHVETLRYGTLWRARGDAQAAPLGIVE
                     AKAILRDKDRFFQPMEYGAAVATIEAAQRAISAAFGPLQVDFTCYRTPFSLLNMEEIT
                     RDAHPDRFPFDHYVNQVLIPTLQRECIDVVGLSACFPGQLQVAYAFAIKIKAALPHIH
                     VTAGGPGFTQLFIRLKGDMLRRAMGPLDSAVVFEGEHTLLRLLTALDDDLPLADVPNV
                     VLRDAQGGAGYLSGHGMEDLRALPAPDFDGLPLDLYLSPKLVLPYDPTRGCYWGKCTF
                     CHYGLAEVGTAAYRERTVEDAVSHLLGLSQKYKTHTFYFSQDSVAPKTLLKLADALRE
                     KGSPLRWATDLKPEKYLTEDRALALRQGGAVACALGVESAAPRVLRLIDKGQPIDVVR
                     DVIDRLAQSDVAVEAMCFTDFPTESGDEAMATLRFLQASRDDVALFIVGRFGLTHGAR
                     VAQEPASFGLSEVFSLLGDDFQTGLFFTEKKQAKRPRDHARIDEALDKLSSTYLLRHY
                     PWAGALSTAHTILYYECYGRNIFRSLADQGVPRDGRGILGKKNLSASAHYDLSATEQA
                     EEVEQQMWQHLIYERRQVSSALYHELASQAPTLFPSPQRYRYAAGEDPVPQLRFRGPR
                     LGRRPAPHVPGAYK"
     gene            4626..5822
                     /locus_tag="PKL17_13590"
     CDS             4626..5822
                     /locus_tag="PKL17_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45815.1"
                     /translation="MMPHIGTAQAAPTTPSPNQPFVVTAPWALEGNYRPVMEGPPPKW
                     QPPALSEAAPPSPLSEQQRQIVQSTKSEKLPEPTWARFVMSNEWRHDVVFPKLNELGG
                     VFVGVATDQNYTMAAASRAQLLITMDYDADVVDTHRIYHHFISASPTADELRSYYTEA
                     GSQKAVAHLQKVAATPEEANRLTKVYHRYRQRLALYLHQVAHVRVGQRKPTWLGDPAA
                     YSYIRSLVQSGRVMAVQGDLNGAVALRSIGDTARALNLPVRVVYTSNAEGFFRYTQAF
                     KDNIASLPHDEKSVILRTYKRGMAAPEGDLWHYNLHKIDDFLARIAMPGYSTVAAVMT
                     DIRTTSEGKKRVESIGVSYYDESIPKTQVEKPKATPAKPTSPAQPAKPAATASDKGTG
                     PLAARL"
     gene            5900..7216
                     /locus_tag="PKL17_13595"
     CDS             5900..7216
                     /locus_tag="PKL17_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK45816.1"
                     /translation="MTTLPKGPGKLALLRMMFDLTYTLADMARDYGDPFTLPTAFGPM
                     VVAVSPEGNKEIFSADPDIFSASAADSFGRVIRNSVLVTSGEEHRRQRRLLTPPFHGQ
                     RMRSYGNLMKTAAKEWTERLAPGNPFQMIYTTQAITLDVIIYAVFGVDHGQRVEQFRT
                     DLLDVLGSFTPLIFLRPLQHEFGGIGPWARFQRGFRKLADHVAALSAEYRQKLDGRED
                     ILSLLLSTKDEEGKGLSAEEILDQLVTIVFAGHETTAVMLAWAFYLLHKYPDQLARLH
                     EEIDPLPLETEPEAIAKLPFLEAVINETLRLYPPVHVIHRKLKKPMRLSGCELPAGTI
                     VAAGAFATHRLSSIYPEPDKFVPQRFLHRTYSPFEFLPFGGGARRCLGAAFATYEMKQ
                     VLFVMLKQKRFELLETEEVRFAHRAGTVGPKGGIRMMVAREQKISS"
     gene            complement(7213..8250)
                     /locus_tag="PKL17_13600"
     CDS             complement(7213..8250)
                     /locus_tag="PKL17_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45817.1"
                     /translation="MSQSASFFVLVVDDEPLARMIHLTAIAKLDDVSVIGASSVAEAL
                     AVIGQRAPQVVVLDMQLQDGTGIDVMSRLAEERHPVVLIVVSAHIDKFRHRLGSGNNI
                     FLLSKPAPHSELLRIIESARRASQKPSPFSPIDYVQLACMGMHSVVVDCIGNEVCGEI
                     IIHNGVLWAAKDEQGEGVPSFIRMVLADGALIRVLPARDPLPARSIEDSWESLILDAM
                     REKDEAERVAPAQKRVKSSPSLPMPAPLEVPKGEAPKAKKDDSVSQPAPTAPTAPSAA
                     SSHSGRRTVLSDEQRATFDQYVERGVRAVIERSYDAAIAAFEQAQAMWPDEPLVRHRL
                     ERLRMLQKKVV"
     gene            8454..11303
                     /locus_tag="PKL17_13605"
     CDS             8454..11303
                     /locus_tag="PKL17_13605"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK45818.1"
                     /translation="MVQARAKQPLGEGLARLVAFSLVVFVVAVFGIYVTRQSGRIASI
                     WLVNAVQLAMLVRNPRMNRRAVMGSGLAANLLADLLIGDRFIIALSLSLSNTLEVAAA
                     NSLLLRLGIHDGEKLIRWPGLPRFFLACGIVAPALSATAATVALGLLGHTAKADLWSS
                     WYAADALGLLILVPILCATQLSDLRRLFAWPKVLETLTLLAVVALAAGAAFGLNRVKL
                     VWLIFPSTLLATMRMGLSGTTLSVLLSSVIGTVLVLSGKTLSTGNVPWSLRSDIAFLQ
                     LILGGQALTLLPVGSILQALWDAQRRNREMTASLVTANRLNQKMFEASPAGIMIFDED
                     GTFLRVNESAARIVGLTVDEMLALNYLKLPSWEKSGLLAAANRAMERGDVVRGESHTI
                     STGGKEIFIEYVFKSFEENQHRRLLLTIIDISERTKVQAAQAQTQRNIQTILDNLDAL
                     VFFWDAELRCRYANHKAREWFGWPPETLPGRHASEVMGAAWFADAKERFDAALRNEPQ
                     RYQRTLQTLNGPLEALISMIPEADDGKVSGLFVLSVDVTQLKEAERGAQAASLAKSEF
                     LANMSHEIRTPLNSVIGYSTLLLDTPLSPDQQEHVSAIRTSGDALLSQINAILDLSKI
                     EAGKLELELIPCELRSAIEDSIEILAEAARKKGLMLSYDLSPDCPTQWLCDPGRLRQV
                     LINLIGNAVKFTDAGDVTVRVTPIATATRPQLQFEIEDTGPGINPADVPKLFQPFSQA
                     DASMSRRHGGSGLGLFVCRRIIESLGGTIGVRAGKEVGTIVSFTLPLANETHAVSESD
                     TLPARCLGRQVLLIGKHVPTRQQLSQMLAAMGLDSLAFSDPESALTAARYHPQHVGAP
                     IKQPLLAVLIDSISDEKARTMAQTLRGHPELEQLPVVRLVGQVDSGNSSSAPAGAPPS
                     ICCVAPSVINDFCASCGTWSDRPPPSENVVAIDAH"
     gene            11324..12130
                     /locus_tag="PKL17_13610"
     CDS             11324..12130
                     /locus_tag="PKL17_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45819.1"
                     /translation="MHPPRLLLAEDNPANQRLAMLMLQKLGCRVDVAATGREALTMAQ
                     RFPYDLIMMDCQMPEMDGLQATVEIRKLPPPEALVPIVALTANAFRSDQERCLAVGMN
                     DFLSKPITVEQLLPVLRRWLPTQFPSTDGTPSTPSIFSRSDRSKSSDVQHEVQRVKAR
                     MAELRSILDEGALKQGRALAYQDWEAQLAGAERALADGKLAQVGKHFHRLAGSALEMG
                     AKELAERCRAIETACKQEDPAAVAKLYPELLSFYRSVLVALAAAGLPMNE"
     gene            complement(12152..14905)
                     /locus_tag="PKL17_13615"
     CDS             complement(12152..14905)
                     /locus_tag="PKL17_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNK45820.1"
                     /translation="MGFLDLFRPRWKHSDPAIRIEAVKSLTPEDLVELGHVVKRDKDA
                     RVRRLALKKISDPALLEEVAEHDPDESLRRDAADKRSELLLASALSDEDESESLDAVS
                     HLSPPRVLVDVVCRAAFPAVQQAALERLRDGKLLADVVRKCKDAQLRKAALAAIDDES
                     VLADLACSDGPKDLLLEALSRVGDPTQLHHVVSRAKLKVLRQAAEQKLPHGAPATSKL
                     EKNATAKGAAVHKTAAAAPAGKTAPKPAVDPTVAEREELCRKLETAAKTDDFEDSEAF
                     LSGLRKRWAELPTIATANPLHKRFERAASRYIERREVFQKRQAAAARGAGQVVQSTSA
                     SAQALLDRMAQIEKAEVEAKEQAELRRREQAVRDEEARKLQEERRGVEDRLRSEQQQK
                     REAIQKARQAERDAELAKEGAERKEREKQRGENQEKNLEKLLGVAQKLVELAEKEDLG
                     LKQAEQTLKEAQETVMTLTSLPVDKRREARQRYDEARSKLVIRVQALREQKDWERWAN
                     VPRLESLCAQVDELLHVVKNDPDVDPQAAADHLKKLQAEWKTVGAAPKEKSEALWQRF
                     KSTSDLVYELVRSAGEAERAENLKAREALIAKIEAFVEITDWKAASETVKGWQADWKS
                     LGPVPHAAKEQGEELWKRFRAACDVFFEKRKAHHAELDEGRQENLRKKEELCIRVERL
                     TMSQDFKAATDLIKKFQAEWKLIGPAPKEQNDALWQRFRKGCDAFFERRKAMLDKLDA
                     ERQGNQKKKEEVCVKAEELANREDIAQDTAEAELKQLMADWRRIGPAPRAEQENLWKR
                     FRAACDKVFQAGRDVELPPQPTGGPKFENKLPLGALLAQLQAEQEATDDEETAKDVAP
                     PVENKAETKAEAPAAEASKLSPSQVSDSWARAATSEWNQIDEIISSGQTPQPDDETST
                     DKK"
BASE COUNT         3050 a         4720 c         4347 g         3183 t
ORIGIN      
        1 gctgctggaa aaacggcggc ggctgcaaca gctatggttg ctggcattca cgctatggtt
       61 cctgcaacag ctacggctgc actgcatacg gaacctgcaa cagctacggc tgcccgaaag
      121 cacgttgagc atccgcacgc ccaccaaacc ttccatagat tcccaccgct tcggaacaac
      181 gatgggaatc cagccgcagc tacagcgggg tgagtttgac gggttggtcg tagagggcgc
      241 cgccttctcc caaatcggtg agcgcagctt cggtgagcgc attggcgcag cggccatcgc
      301 gcagatgtcc gcctttggga acgatcgcca cgtcttttcg ctgcgctggg tcatgaagga
      361 cgcgcacctg catcttccca atcacggaag cgagcacacc tgttccgcca tggggaattc
      421 cgtttgcact gtccggatgc accgtcagct ctgcaagtcc ctgctgcggc ctgacccact
      481 gcgaggactg cgaatccgga gtcgacagcg ccatcaggag cagcggatat tctgctgatg
      541 gtttggccgg cgaaggagga agggaatcga tcagattcac ttttcccgac ggtgtcgcaa
      601 actttcgatc ggcaaacaga accttttcgg ccagcggatt tcgcagtggc cccgcttcca
      661 gtgtatcgac cgtgattccg tgtggcccaa gctttggcgc gatcatcttc gctttccagt
      721 cgcgcggact gccagccaca acatcggcga gcccgacgcg agcggccagc gcttggatga
      781 tctcgagatc gcttttcaca tcgggaggcg gcggtgaaac cggacgcgaa acgcccagat
      841 agtgatgtcc ataggagccc aacaggtcat cactttccag cagcgttgtc gtcggaaaca
      901 ccacatgcgc aagttccgcg gtatcggtca gcagatgatc acagaccacc agcaaatcgc
      961 ggctccgcag tgcatccgct gtggtttggg aatcgggcag catcgcaacc ggattgcctg
     1021 ccgtcaccca caccgcacga atcggcggat cggacatgtt ctggacttcg cgaccaaaca
     1081 gcggttcaca gacagtccgc ggcgcgcgtc cttccacgaa agaggaatcg aacgcccccc
     1141 gtcgcttaaa gtaaaaggaa gcccccccac cggggattcc caagttgcca gtcagcgccg
     1201 ccagcgcatc gagcgcacgc accaccgccg cgccatttcg ccgccttccc attccccaac
     1261 cgatcagaat ggtcgccggt ttgtcctgtc ccagtcgctt cgccaaaagc tgcgcggatt
     1321 cccgactgac atcggcttcc gcaagccagt cctcaagcga gcggctgtgc gcgagggcgc
     1381 gaaattcacg aatcttgtca caataggaat cggcctgtgg atcgatccag ttttgcgcaa
     1441 acagaatctg tccgaccgcc atcgcgagcg caaagtcccc gcccggcctg acttgcacaa
     1501 agtcctgaca cagactcgct gacttgtggg ccaccggatc aatcagtgca agctgcgccc
     1561 ccgcacgtcg acaatccttg agcagcggca gaagatgcgg cgatgaaatg aagggatttt
     1621 tcccccacaa aaggatgctc ttcgcgtttt gcagatcgaa aaaatcgctc gattcttcga
     1681 tgccaaagtc ttcgatctgc gcggcatcgc ccgcccccga acagatgtcg ccacgcttga
     1741 ctgtcaccgg cccaaagtgt tcccaaaagc ggtcgacgac cgcgcccatc attcccagcg
     1801 tgccaccgga acgatagtaa aagatcgacg caggaccgga ctccttgcga atctgtagca
     1861 gcttgtctgc ggtataggaa agcgcttctt cccacgacac cggcagcagc ttgccgctcg
     1921 cggagctacg caaaagcggc ttggtcagtc gctggctgct gtactgggtt tccagaaaat
     1981 gactggtccg atagcaaagg aatccctgcg tgacgggatg atcgggatcc cccgccaagt
     2041 gggtaattcg gccatcttcg acggtggcaa caatcgagca ggcatccgga cagtcacgat
     2101 tgcagatggt ctttgcggtg gttttcactt gtaagctccc ggcacatgcg gcgctggacg
     2161 acggcccaga cgcggacccc gaaaacgaag ctgcggaacg ggatcttccc ccgcagcata
     2221 gcgatagcgc tgtggcgatg ggaacagcgt cggtgcttgg ctcgccagct cgtgatacag
     2281 cgcactcgac acctgccgcc gctcatagat caggtgttgc cacatctgct gctcaacctc
     2341 ttccgcttgc tctgtcgcgg ataggtcgta gtgcgcactg gctgataaat tctttttccc
     2401 caagatgccg cggccatcgc gcggaactcc ctggtctgcc aagctccgga atatattacg
     2461 cccgtaacac tcgtaatata gaatcgtatg tgccgtcgac agcgcccctg cccatggata
     2521 gtggcgcagc agataggtcg aagacagctt gtcgagcgct tcatcaatgc gcgcatgatc
     2581 acgcggtcgc ttggcttgtt ttttttcggt gaaaaacagt ccggtctgga agtcatcccc
     2641 cagaagtgaa aacacttccg aaagaccaaa ggaagccggt tcctgcgcca cccgcgcgcc
     2701 atgtgtcaga ccaaaacgtc cgacgataaa cagcgccacg tcatcacgcg atgcttgcaa
     2761 aaaacgcagc gtcgccatcg cttcatcacc gctttccgtc ggaaaatcgg taaagcacat
     2821 cgcttcgacg gctacatcac tttgcgccaa gcggtctatc acatcgcgca ccacatcaat
     2881 cggctggcct ttgtcgatga gccttagcac ccgcggagcc gccgattcga caccgagcgc
     2941 acacgccacc gctccgcctt ggcgcagcgc caacgcccga tcttccgtca gatatttttc
     3001 cggtttcaga tccgtggccc agcgcagcgg actccctttt tcacgcagcg cgtccgccag
     3061 cttgagcagc gttttgggcg ccaccgagtc ttgcgaaaaa tagaaagtgt gtgtcttgta
     3121 tttctgcgaa agtccgagaa gatgagacac cgcgtcttcg acggtgcgtt cgcggtacgc
     3181 tgcggttcct acttccgcca gaccgtagtg acagaacgtg cacttgcccc aataacagcc
     3241 gcgggttgga tcatagggaa gcaccagctt gggcgataga tacaggtcga gcggcaagcc
     3301 atcaaagtcc ggcgcaggca gtgcccgcag gtcttccatt ccatgaccgg acagatatcc
     3361 tgccccgccc tgggcatccc gaagcaccac gttcggaacg tccgcaagtg gcaaatcgtc
     3421 gtcgagcgcg gtcaaaagtc gcagcagtgt atgttcccct tcaaacacca ccgcggaatc
     3481 aagcggaccc atcgcacgcc gcagcatgtc ccccttgagt cgaatgaaaa gctgggtaaa
     3541 tcccggccct cccgcggtca catgaatgtg cggaagcgcc gctttgatct tgatcgcaaa
     3601 cgcataggcg acttgcagct gaccgggaaa acaagccgac agaccgacga catcgatgca
     3661 ctccctttgc agggtgggaa tcaagacctg attcacataa tgatcaaagg ggaatcgatc
     3721 aggatgcgcg tcgcgcgtga tctcttccat gttgagaagg gaaaacggcg ttcgatagca
     3781 agtgaagtcg acctgcagcg gaccaaacgc cgccgagatc gcacgctgcg cagcttcgat
     3841 ggttgccacc gcagcgccat attccatcgg ctggaaaaag cgatctttgt cacgcaggat
     3901 ggctttggct tccacgattc ccagtggagc cgcctgcgcg tcgccacgag cccgccacag
     3961 cgtcccataa cgaagcgttt ccacatgggt gagctgcggc tttttttcaa gctcagcaag
     4021 ctgcgtttcc aagcgatgcg ccacgtcggt aagccggtct aacgacagca gaccatccca
     4081 cgcttcgatg ttggcgtcga tcggcagcac ttcgcgtccg tgactgcgca aaaagccggt
     4141 caacatcggg accgccagat acggagccgt cggatcgcac gtcggtggat aaatgagcgc
     4201 gagctttttt gccatgactt agaaggggcc aatcatccaa aagccggcgc cattgtcaaa
     4261 gcccttttcc atacatcggt acggactgtg gttgggaatg ggtaggaaga ctcccaaacg
     4321 attccttttt gcctgtttgt cgcacaggta ggcgaccgct tgggacgacc tttttttgtc
     4381 aaacaaaacg cgagcaaaac ttcccaattt gagacgagaa acaagcttcc caactcagct
     4441 tgtcagcgac tcctttgcca gcgccggaag gccccctgtt tggccccctt ttttgtttca
     4501 gaattttttc acgcaagctc tttaccggct cgtttttgaa gtggtacgat cggaatgcga
     4561 acatgcgtac acatatctcc tccccccgtc ttgtacacca tgctctcctg accttggcgc
     4621 tggccgtgat gccgcacatt ggaacggccc aggccgcgcc gaccactcca agcccaaacc
     4681 aaccgtttgt ggtgacggca ccgtgggcgc tggaagggaa ttaccgtccg gtgatggaag
     4741 gaccgccgcc caaatggcag ccgccggccc tttcggaagc ggctccaccg tcgccgctca
     4801 gcgaacagca gcgccagatt gtgcagtcca ccaaaagtga aaaactgccc gaaccgactt
     4861 gggcgcgctt tgtgatgtcc aatgaatggc gtcacgatgt ggtgtttccc aaactgaacg
     4921 agctgggtgg tgtgtttgtt ggtgtcgcga ccgaccagaa ctacacgatg gcggcagctt
     4981 cgcgggccca gcttctcatc acgatggact atgacgcaga tgtggtggac acccaccgca
     5041 tttatcatca cttcatcagc gcatcaccga cggcggatga gctgcgttcc tattacaccg
     5101 aagcgggctc ccaaaaggct gtggcacatc tgcaaaaagt cgcagcgacc ccggaagaag
     5161 cgaatcgact gaccaaggtg tatcaccgct atcgtcagcg cttggcgctc tatctgcacc
     5221 aagtcgcgca tgtgcgggtc ggacagcgca agccgacgtg gcttggcgat ccagcggcct
     5281 attcctatat ccgcagcctg gtgcaaagcg gtcgcgtgat ggcggtccag ggcgacttaa
     5341 atggcgcagt ggcgcttcgc agcattggtg atacggcgcg cgcgctcaac ctgccggtgc
     5401 gtgtcgtgta cacctcgaat gccgagggat ttttccgtta cacgcaggcg tttaaggaca
     5461 acatcgcatc gcttccgcac gatgaaaagt cggtgatttt gcggacgtac aagcgcggca
     5521 tggcggcccc cgaaggcgat ctgtggcact acaacctgca caagatcgat gactttttgg
     5581 cgcggattgc gatgccaggc tattcgacgg tcgcagcggt gatgacggac attcgcacga
     5641 cctcggaagg caaaaagcgg gtcgagtcga tcggggtgtc ctactacgat gagtcgattc
     5701 ccaagaccca agtggaaaag cccaaagcga cccccgcaaa accgaccagc ccggcccagc
     5761 ccgcaaagcc ggcagcgacg gcatcggaca agggaaccgg tccgctcgcg gctcgcctgt
     5821 aaagccctct ttacctctct gcccgcctgt gcggtaattg gtacagattc ctaccccact
     5881 ccttacccag gaggcatcca tgaccacgct gcccaaagga ccaggaaagc tggccctttt
     5941 gcggatgatg tttgatctta cctatacgct tgcggacatg gcccgcgact atggggatcc
     6001 attcacactg ccaacggcgt ttggcccgat ggtggtggcg gtgtctccgg aaggaaacaa
     6061 agagatattt tccgccgatc cagacatctt ttcggcatcg gcagcggatt cctttgggcg
     6121 ggtgattcgc aattccgttc tggtcacaag cggagaagaa caccgaaggc agcgacggct
     6181 cctgacccct ccttttcacg gtcaaaggat gcgcagttac ggcaacctga tgaagactgc
     6241 ggcaaaggaa tggaccgagc gactggcgcc tgggaatccg tttcagatga tctacaccac
     6301 gcaggccatt acgctggatg tgatcattta cgcagtgttc ggtgtcgatc atggccagcg
     6361 agtagagcag tttcgcaccg acttgcttga tgtgcttggt tcctttacgc cactgatttt
     6421 cctgcggcca cttcagcacg agtttggcgg catcggtccg tgggcgcgct ttcagcgagg
     6481 cttccgcaaa ctggccgatc atgtggccgc actttcagcc gaatatcgcc aaaagctcga
     6541 cggaagagaa gacattctgt ccttgcttct gtccaccaaa gatgaagaag gaaaaggtct
     6601 ttccgcagaa gaaatcctcg atcagcttgt cacgatcgtg ttcgctggac atgaaaccac
     6661 ggcggtcatg ctggcgtggg cgttttatct tctgcacaaa tatccagacc agcttgcgcg
     6721 gctgcatgag gagatcgatc cgctgccgct agagaccgaa ccagaagcga ttgccaagct
     6781 gccgtttctg gaagccgtga taaacgaaac gctgcggctg tatccgccgg tgcatgtgat
     6841 tcatcgcaag ctaaaaaaac cgatgcggct gtcgggctgc gagcttccgg cgggcaccat
     6901 cgttgcagcg ggagcctttg caacccatcg actttcatcg atctatccgg agccggacaa
     6961 gttcgttccc cagcgattcc tacaccggac ctattcgccg tttgaatttt tgccgtttgg
     7021 cggtggagcc cgtcgctgct tgggtgcagc gtttgctact tacgaaatga agcaagtgct
     7081 gtttgtgatg ctaaaacaaa agcgcttcga gcttctggaa accgaagaag tccgctttgc
     7141 tcaccgcgcc ggaacagtcg gtcccaaagg cggaatccgc atgatggtcg cgcgcgaaca
     7201 gaagatcagt tcctagacca ctttcttctg caacatccgc agtcgttcca agcgatggcg
     7261 gaccaggggt tcatctggcc acatcgcttg cgcctgctca aacgcggcaa tcgccgcatc
     7321 gtagcttcgt tcgatcacgg cgcgcacgcc acgttccaca tactgatcga aggtggcgcg
     7381 ctgttcatcc gacaaaacgg tgcgccgtcc agagtggctg gatgcagcgc tgggagcggt
     7441 aggagcagtg ggcgctggct gtgaaacgga gtcgtctttt ttggctttgg gagcctctcc
     7501 cttgggaact tcgagcggtg ctggcatcgg cagagaagga gacgacttga cgcgcttctg
     7561 cgctggcgca actcgctctg cttcatcctt ttcacgcatc gcatccagga tcagcgattc
     7621 ccaactgtct tcgatgctgc gcgcaggaag tggatcacgc gccggcagaa cacgaatcaa
     7681 agcgccgtct gccagcacca tccggatgaa cgaaggaacc ccttcacctt gctcgtcttt
     7741 ggccgcccac agaaccccgt tgtgaatgat gatttcgccg cagacttcat tcccaatgca
     7801 gtcgaccacg acgctgtgca ttcccataca agcaagctgt acgtaatcaa tgggcgaaaa
     7861 ggggctcggt ttttgggaag cgcgccgcgc actctcaatg atgcgaagaa gctcgctgtg
     7921 cggtgccggt ttcgagagca ggaagatgtt gtttcccgaa cccaagcgat ggcggaattt
     7981 gtcgatgtgg gcacttacga caatcagaac gaccggatgt ctttcttccg caagccgcga
     8041 catcacatca atgccggtgc catcctgaag ctgcatgtca agcacaacga cttgcggtgc
     8101 tcgctgcccg atgaccgcca atgcttcagc gacgctgctg gccccgatca cactgacatc
     8161 atcgagcttg gcaatcgcag tcagatgaat cattcgggca agcggttcat catcgactac
     8221 cagcacaaag aaagacgccg attgagacat caggaatttc tttctacgaa atgctagaga
     8281 tgttagcgca tcttgcgcat tggcgtctac gactcctcgc aggtcggcgc acagactttt
     8341 gccgcattcc tttccggaca gagaatcaca cccacaccag cacgaccccg cgtctgaaaa
     8401 atccccagaa gaagtcgaga cagaagcccc ggagatcgat catcctaaga tccgtggtac
     8461 aagcacgcgc gaaacagcct ttgggggaag ggctcgcccg cttggttgcc ttttcgctgg
     8521 tggtgtttgt cgtcgctgta ttcggaattt acgtcacaag acagagcggt cgcatcgcat
     8581 ccatttggct ggtcaacgcc gtccagctcg cgatgctcgt gcgcaatcca cggatgaacc
     8641 gtcgggcggt gatgggttcc ggactggctg cgaatctgct cgccgatctg ctcatcggcg
     8701 atcgcttcat catcgcgctg tctttgtcgc tgtccaatac gttggaagtt gcagcggcca
     8761 acagtctgct attgcggctt ggcattcacg acggagaaaa actcatccgt tggcctgggc
     8821 tgccgcgttt tttcctcgcc tgcggaatcg tggccccggc cctgtcggca accgctgcga
     8881 ccgtcgcatt gggcctgctc ggtcacaccg ccaaagcgga tctgtggtcg tcttggtatg
     8941 ccgccgatgc gctgggtctg ctcatcctgg ttcccatcct gtgcgcgacc cagctttccg
     9001 atctgcgtcg gctgtttgcc tggcccaaag tgctggaaac gctcactctt ttggcggtcg
     9061 tggcactcgc agccggtgcg gcatttgggc taaaccgcgt caagctggtg tggctgatct
     9121 tcccgtcgac gcttctggcg acgatgcgga tggggctatc ggggacaacc ttgtcggtgc
     9181 tgttgtcgtc ggtgatcgga acggtgctgg ttctttcggg caagacgcta agcactggaa
     9241 atgtgccgtg gagtctgcgc agcgacatcg cgtttttgca gctcatcttg ggtggacaag
     9301 cgctgacgct tctgccggtg ggatcgatct tgcaagcgct gtgggacgca cagcggcgca
     9361 accgggaaat gaccgcatcg cttgtgacgg caaaccggct caatcagaaa atgtttgaag
     9421 cgagcccggc aggaatcatg atcttcgacg aagacgggac attccttcgc gtcaatgaaa
     9481 gtgcggcgcg aattgtgggt ctcaccgtcg atgaaatgct cgcgctgaac tacttgaagc
     9541 tgccgtcatg ggaaaaatcg ggcctgctgg cggcggcaaa tcgagcgatg gagcgaggcg
     9601 atgtcgtccg cggcgagagt cataccatct cgacgggagg caaagagata ttcatcgagt
     9661 atgtcttcaa gtcgtttgaa gaaaaccagc accgccggct gctgctgacc atcatcgaca
     9721 tcagcgagcg aaccaaagtc caagcagccc aggcgcagac acagcgcaac atccagacca
     9781 tcctcgacaa tcttgatgcg ctggtgtttt tctgggacgc agagctgcgc tgtcgctatg
     9841 ccaatcacaa agcgagagag tggttcggtt ggcccccaga aaccctgccg ggcaggcatg
     9901 ccagcgaagt gatgggagca gcctggtttg ccgatgccaa ggagcggttt gacgccgcgc
     9961 tgcgcaatga accgcagcgc tatcagcgga cattgcagac cctgaatggt cccctggaag
    10021 cactgatttc catgattccg gaagccgacg acggcaaggt gtccggactc ttcgtcctgt
    10081 cggtggatgt cacccagctc aaagaagccg agcgcggtgc ccaagcggcc agcctcgcca
    10141 aaagcgagtt cctcgccaac atgagccacg agatccggac gccgctcaat tcggtgattg
    10201 gctattcgac gctgctgctc gatacgccac tgtcgccgga tcagcaggaa catgtctcgg
    10261 cgattcggac ttcgggcgat gcgctcctgt cccagatcaa cgcgattttg gatctgtcga
    10321 agatcgaggc tggaaagctc gagctggagc tgattccgtg tgagctgcgt tccgcgatcg
    10381 aagattcgat tgagattttg gccgaagcgg ctcgcaaaaa aggcctgatg ctaagttacg
    10441 atctgtcccc ggactgtccg acgcagtggc tgtgcgatcc gggccgactg cggcaggtgc
    10501 tgatcaactt gatcggcaat gcagtcaaat tcaccgacgc aggcgatgtc acggtgcggg
    10561 tcacaccgat tgcgacagcg acgcggccac agcttcagtt tgaaatcgaa gacaccggac
    10621 ccggcataaa ccccgccgat gttccgaagc tgtttcagcc gttttcgcag gccgatgcct
    10681 cgatgtcccg tcgtcacggc ggcagtggcc tggggctttt tgtctgtcga cggatcatcg
    10741 aaagcctggg cggtaccatc ggggttcgtg ccggaaaaga ggtcggcacc atcgtttcat
    10801 tcaccctgcc gctcgcgaac gaaacccatg cggtgagcga aagcgatacc ctgcccgccc
    10861 gctgtttggg tcgccaggtg ctgctgatcg gcaaacatgt cccgacgaga cagcagcttt
    10921 cgcagatgct ggccgcgatg ggtcttgatt cactggcgtt ctccgacccc gaatccgcct
    10981 taacggcagc ccgctatcac ccgcagcacg ttggagcgcc catcaaacag ccgctgctcg
    11041 cggtgctgat agacagcatc agcgacgaaa aagcccgcac gatggcccaa accctgcgtg
    11101 gacacccgga gctcgaacag ctcccggtgg tgcggctcgt cggtcaggtc gacagcggca
    11161 acagcagcag cgcaccagcg ggcgcccccc catcgatctg ctgcgtcgcc ccgtccgtta
    11221 tcaacgactt ttgcgcatcg tgcgggacct ggtcggatcg tcccccgcca tcggaaaacg
    11281 tcgtcgcaat cgacgcccac tgagcaccga aatctccatc accatgcatc cgccgcggct
    11341 gctgctggcc gaagacaatc cggccaatca gcgactcgcc atgctgatgc tgcaaaagct
    11401 cgggtgccgg gtcgatgtcg cagcgacggg ccgagaagca ctgacgatgg cgcagcgttt
    11461 cccgtatgac ctcatcatga tggactgcca gatgccggaa atggacggac tgcaggccac
    11521 cgtcgaaatc cgcaagctgc caccgccgga agccctggtc cccattgtcg cgctgacggc
    11581 caacgctttt cgatccgatc aggaacgctg cctcgcggtg ggaatgaacg acttcctgag
    11641 caagccgatc accgttgaac aactgctgcc ggtgctgcga cgctggctgc cgacccagtt
    11701 tcccagcaca gacggtacgc ccagcactcc gtctatcttc tctcgatcgg accgcagcaa
    11761 gtcgagcgat gtccagcatg aagtgcagcg agtcaaggcg cgcatggccg agcttcgcag
    11821 catcctcgac gaaggcgcgc tcaagcaagg acgcgcactc gcctaccaag actgggaagc
    11881 gcagcttgcc ggagccgagc gagcgctggc cgacggcaag ctcgcccaag tcggcaaaca
    11941 tttccatcga ctggccggta gtgcgctcga gatgggagcc aaagagctgg ccgagcgctg
    12001 tcgcgccatc gaaacagcgt gcaagcagga agacccagcc gccgtcgcca agctctatcc
    12061 cgagctgctg tcgttttacc gcagcgtgct cgtcgcgctc gcggcagccg gtctgccgat
    12121 gaatgaatga aaaggaaaac cgcgcgccgc gctacttctt gtcggtgctg gtctcgtcgt
    12181 cgggctgcgg ggtctgtcca gaggagatga tctcgtcgat ttggttccat tcgctggtgg
    12241 cggcacgcgc ccagctatcg gaaacctgac tcggcgacag tttcgacgcc tcagccgccg
    12301 gtgcttcggc tttggtttca gccttgtttt cgacgggtgg ggccacgtct tttgcggtct
    12361 cttcgtcgtc tgtcgcttct tgttcagctt gcagctgcgc caacagcgcc ccgagcggca
    12421 gcttgttttc aaacttgggt ccgcccgtcg gctgcggtgg tagctcgaca tcgcgcccgg
    12481 cttggaacac cttgtcgcag gcggcccgga agcgcttcca caggttttcc tgttcagccc
    12541 gcggtgccgg tccaatccgt cgccaatcag ccatcagctg cttcagctcg gcttcggcgg
    12601 tgtcctgcgc gatgtcttcg cggttggcca gctcttccgc cttgacgcag acctcttctt
    12661 ttttcttctg gttgccctgc cgttcggcgt cgagcttgtc gagcatcgcc ttgcgccgct
    12721 caaagaacgc atcgcagccc ttgcggaacc gctgccacag cgcatcgttc tgctctttgg
    12781 gggcaggccc gataagcttc cactcggcct ggaacttttt gataaggtcc gtcgctgcct
    12841 taaagtcctg cgacatcgtc agccgctcga cgcgaatgca cagctcttct tttttgcgca
    12901 ggttttcctg acggccttcg tcaagctccg catgatgggc tttgcgcttt tcaaaaaaga
    12961 catcgcacgc cgctcggaac cgcttccaca gctcctcgcc ctgctctttg gccgcgtgcg
    13021 gcaccggccc gagcgacttc cagtccgcct gccagccttt gaccgtttcc gacgccgctt
    13081 tccagtcggt gatctcgaca aacgcttcga ttttcgcaat cagggcttcg cgcgctttca
    13141 ggttttcggc ccgctcggct tccccggcgc tgcgcaccag ctcatagacc agatcggatg
    13201 tcgacttgaa gcgctgccac agggcttccg atttttcctt gggcgccgca ccgacggtct
    13261 tccactcggc ttgcagcttc tttagatggt cggctgccgc ctgcggatcg acatcgggat
    13321 cgtttttgac gacgtgcagc agctcatcca cctgcgcgca cagcgactcc agccgaggca
    13381 cgttcgccca gcgctcccag tctttttgct cacgcagggc ctgaacccgg atgaccagct
    13441 tgctgcgcgc ttcgtcgtaa cgctgacggg cttcgcgccg tttgtcgacc ggcagcgacg
    13501 tcagcgtcat caccgtctct tgcgcttctt ttagcgtctg ctcggcctgc ttcagcccaa
    13561 ggtcttcttt ttcagccagc tcgacgagct tttgcgccac gcccagcagt ttttcgaggt
    13621 tcttttcctg gttttcgccg cgctgctttt cgcgctcctt gcgctcggcg ccttctttgg
    13681 caagctctgc atcgcgctcg gcctgacggg ctttttggat cgcctcgcgc ttctgctgct
    13741 gctcggaccg cagcctgtcc tcgacgccgc gccgctcttc ttgcagcttg cgcgcttctt
    13801 cgtcgcgaac ggcctgttcc cggcgccgca gttcggcttg ctctttggct tccacctcgg
    13861 ctttttcaat ctgcgccatc cgatcgagca gcgcctgcgc cgaagcgctt gtcgactgca
    13921 ccacctgacc tgcgccccgc gccgccgcgg cttgccgctt ttggaagact tcgcgccgct
    13981 cgatgtatcg agaagctgcc cgctcaaacc gcttgtggag cggattcgcc gtcgcaatcg
    14041 tcggcagctc ggcccagcgc ttgcgcagtc cggaaagaaa cgcctcggaa tcctcgaagt
    14101 cgtcggtctt cgccgcggtt tcgagcttgc gacacagttc ctctcgctcg gcaaccgtcg
    14161 gatcgactgc cggcttcggt gcggtcttgc ccgccggggc tgccgctgcc gttttgtgca
    14221 ccgcagcgcc cttggctgtc gcgttttttt ccagcttgct cgtcgctggt gcaccgtgcg
    14281 gcagcttctg ctctgcagcc tggcgcagca ctttcagctt ggcgcggctt acgacatggt
    14341 gaagctgcgt cggatcgcca acccgcgaaa gtgcttccag cagcaggtct ttcggtccgt
    14401 cgctgcaagc cagatcggcg agcaccgatt catcatcgat cgccgccagc gccgctttgc
    14461 gaagctgggc atctttgcac ttgcgcacca cgtcagccag aagcttgccg tcgcgtaaac
    14521 gttccagcgc ggcctgctgc accgccggaa acgcagcccg acagacgaca tcgaccagca
    14581 cgcgcggcgg cgacaagtgc gacacagcat ccagactttc cgattcgtct tcgtcggaaa
    14641 gcgcgctcgc cagcaaaagt tcgctgcgtt tgtccgctgc gtctcgacga agcgactcgt
    14701 cgggatcgtg ctcggcgact tcttcgagca gcgctggatc actgattttt ttgagtgcaa
    14761 gccgacgcac acgcgcatct ttgtcgcgct ttactacatg gcccagctcg acgaggtctt
    14821 ccggggtcaa gctcttgacg gcctcgatgc gaatggccgg gtcgctgtgt ttccagcggg
    14881 gtcgaaaaag gtcaagaaat cccatcgttt ggcgctccgt aacttgggac gaaatataca
    14941 cgattggttg ccctcgccgc ctcgctgaaa aaatactctt gcgcactttg cgtgccgaca
    15001 gtgagcaatg cgggtgggct tggtatatga tgaggccact tcgcaaagcc ttccgaagga
    15061 ctacctgtcc catgcgttca ttactgttct cgacgctgat atgtgccctg ctgtggcaaa
    15121 ctgggtgtaa acccaattac ccaaactgcg aggcagactc cgactgcccc ggccacgctg
    15181 aaggcaaaga gtggtgtgta aacggcaagt gccaagcttg tcgtccgacg ggcaatgact
    15241 gcggtcctgg caaagcatgc aatggcggtc gctgcgaggc agtgccgggc tggtgcggca
//
LOCUS       DAORGN010000122        15287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1598_length_15287_cov_5.811318, whole genome shotgun sequence.
ACCESSION   DAORGN010000122 DAORGN010000000
VERSION     DAORGN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_15287_cov_5.811318"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3159
                     /locus_tag="PKL17_13620"
     CDS             <1..3159
                     /locus_tag="PKL17_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK45821.1"
                     /translation="QILTALTYLHRREIIHRDVKPSNVLVIPVPESPGFRVKLLDFGL
                     AVTWRRVSISADTVAGTLGYLAPELLQGQIPSVSSDLYSVGVLAYELLVGKHPFPVDG
                     PTERFLYHVKHNAPDFTGLKPAALIPIITQSLAKTPWDRPESAATFLQELVEKGGLLS
                     EGESEAVRDSFLIAAPFTGRENEFCALATELAQSQRGMGAAWLLGGHSGIGKSRLLDE
                     VRAMALIRGFCVARGETAPHGQSPLLLWKTPLRMLCLGISLSDEELSALECLIPDVSA
                     LQGNISPSGPKVVAQLEIPQLAKVLIDILKRQPQPVLLLLEDLQWADEESLTILRTVA
                     SHAVSLPMLIIATYRSEEAPTLPEKLPELSVMQLERLSERSLQQLCTSMMGEQASSSL
                     QSLIERETAGNTYFLVELMRSLAEEAGSLDRVAGHDLLKDIQRGGIMEVLRRRIERVP
                     QRAMPLLRIAAVAGRQLDLAVIEQLLPHAEELIEACASVGVLELHDQHWRFSHDKLRE
                     FVLAALPADSKRELHSWLAETITQVYPDLSSQAMRVAHHHLEAGQLAQAGDCFSLAGS
                     VAISAGSVNEGEQLLSRAIALQTQTGRPPILRLRTYRQLALVRFTLGKNQGADTAIQH
                     AFALAGIRWPHGVGARTIDTVARFVEQTLRQSRHLKATVQHAEEKELYSELLKILPAT
                     EVYLALAQPASAMFCWVHCLRIAETVDDVMAHLQAASFVAYAFSSVGLHTLSAQYLAR
                     SESLSQRSQQAEAMILIERAKAGIALAAGRFGDAISACQHARQQNTTAQELTPTFRIL
                     RVELLAHLLSGNLSSAWSLADELWALSERHDLPQKQTLAVGYRGALWARQGDPVRAAR
                     FLEQASGADTALEDSHHALWFGGQLASCYAKHGQPHRALRLAKTQVQSLRSHSVASIL
                     SALGMYALIETYFALGMESACSVSEASAEVSTIMKQLGQLALRYRPVSPIFELGLFHM
                     LQLKGQVALSIWALRRALVHASALCMPYEEALAHTFLAEQAEKASPAVLLLIPESPQT
                     HRARAQERFEQLGIPIPRQSPT"
     gene            3264..4088
                     /locus_tag="PKL17_13625"
     CDS             3264..4088
                     /locus_tag="PKL17_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45822.1"
                     /translation="MSAIHVDVHPIGSQPRPRTRSLPFAKLALCVFVAGLHAAGCSSG
                     GYRPPVDDKHGTDGGVSDLAITEPGRDGGTLSDGGMGSWDLLPDLTAPPSSDPFDPAS
                     CPGPAWSAASALTTLGTAARAKLADATLMRRTRTCTGTTPATCGAWSTPIPHTQALLT
                     YSGGVTTDYKTFSFPTHLILFAQSGQPKLVVRHESDYRHDATANTRGVVFPLGAFPIE
                     NTYPVIYVWDFAPAPYRYDDLQGLLGGKGQLFAAERCARAVFTDGLTTEIAALYRY"
     gene            complement(4112..4438)
                     /gene="clpS"
                     /locus_tag="PKL17_13630"
     CDS             complement(4112..4438)
                     /gene="clpS"
                     /locus_tag="PKL17_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNK45823.1"
                     /translation="MSKERHQHDDTNVVLDRKVVPKVKPPRMYKVLLHNDDYTTMEFV
                     VAVLETVFHHSNAAAMKIMLHIHHQGVGVAGVFTHEVAESKVAKVMALAREAQYPLLC
                     TLEPET"
     gene            4522..6783
                     /locus_tag="PKL17_13635"
     CDS             4522..6783
                     /locus_tag="PKL17_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNK45824.1"
                     /translation="MRRHLWVAATCAALSFGCEQMPPDVEPKASFSVRESIGQLAVTH
                     APPGATLDLRDEGGNLVGSGVADELGSYLFRGVKPGAGYVLSRRGADPEDRTGKLTVY
                     SVESSRKPQSFYANQKLSPGFGYITTRDGTQLSIYVTLPGPIEKGPYPTVVNISGYSP
                     SQPGKQDDKYAGLCIAYPALCDQPNDPSAMIAAVNGYATVGVNLRGTGCSGGAYDFFD
                     TLQKLDSYDVVETVAAQDFVLHHKVGLTGLSYPGISQLFTGAQRPKSLAAITPLSVIG
                     NMFMTLAPGGVLNSGFALSWITRVLDKAGPYKQGWEQGVVDLGDSICKENQLLHGQKA
                     NLIDVIKAHQYYPPELGDAVNPETFVGDIDVPVFLAGAWQDEQTGPYFVSLLNRFRSA
                     PLTRFHMYNGVHPDGFAPQILIEWKTFLDLYVARKVPQVDQPLRVLVPQLFKDFFGAK
                     MELPPDRFADQPNWEAARKKYEAEPAVRVLFESGGVSTNLGAPESRFQATFPAWPPKT
                     QPVRYYFRQDGSLSTSTPTETSAATQIVFDPNAGTQGNLAPGGDIWALIPKWDWKPLQ
                     TNKAAAWETAALSEDVVMVGTASVDLYVQSTANDADIQVVLSEIRPDGKEQFVQAGTL
                     RVSNRALTRDATPLWPEHSYRETDATPLPAGKWSEARVGIPGFAHAFRKGSRLRLSVG
                     TPGGNHAEWRFQTLPQSPTTQHQIAHSAAQPSSILLPVVSGVTVPPQLAPCPSLRGQP
                     CRSYQALANTPAP"
     gene            complement(6852..8249)
                     /locus_tag="PKL17_13640"
     CDS             complement(6852..8249)
                     /locus_tag="PKL17_13640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNK45825.1"
                     /translation="MRIGVIGTGYVGLVAGAGFADFGNDVVCIDIDEQRIARLLRGDV
                     PIHEPGLPAMVAENAAAGRLRFSTKFADATEDADVILLCVGTPPAADGRADLSQVFAA
                     AEAVGKHLSSEFTVIVDKSTVPVGTAERVRDIIAGVSQRPFAVVSNPEFLKEGSALDD
                     FLRPDRVIVGVGPSAGPAGRSPALSTAEQVDERARVVIRQLYSAVVRKNDRLLFMDAR
                     SAELTKYASNAYLAMRISFMNDVARLCEHIGADVELVRRGMGMDQRIGPSFLFPGVGY
                     GGSCFPKDTRALIHIAEDVGLPLPLVSATESINARQKRRLFDKLLAHFGKREDEPLAL
                     TGKRIAVWGLSFKPETDDVREAPALTLIEKLVALGAEVRAYDPVASETARQSLSATLK
                     EKMSQVEIVASAYDALPDADALCLCTEWRQLRQPDFRKIVRLLRGKLLLDGRNIWDPA
                     QVRAAGLVYQGIGRR"
     gene            complement(8289..9377)
                     /locus_tag="PKL17_13645"
     CDS             complement(8289..9377)
                     /locus_tag="PKL17_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNK45826.1"
                     /translation="MRTPVSRLSVVRSALFASSVLGFSLLIGCEPASSDEWTPVSPSE
                     VEGNDAPLTSVGVLKADAPSDSSIPFEGKADVVLPKQADVLSIQTSVKNQASRGVCSI
                     FSSLGLVESLFKKAGMTTTPDFSEQYLQWLVKAKLNAFPGSSGSSDYYNLKAVNTYGV
                     VAETVWPYEPTQWDVTKDSRCTGTGDGLPNICYTNGDPPAAASSATKYKLPSAGRYVS
                     TSSIKNIIFEKKQGVVVGLDFFYQAWNHRRSALPVSSAYFQKGYVLYPNAEDQAASAK
                     QPAGHSVQLVGYDDDLEIQAMDKDGRPAVDAAGKPIKQKGFYLFKNSWGTANFGLYNS
                     KVPGYGWISYRYVQEFGSAYTADPPKLP"
     gene            complement(9563..10345)
                     /locus_tag="PKL17_13650"
     CDS             complement(9563..10345)
                     /locus_tag="PKL17_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNK45827.1"
                     /translation="MQPQKPTADSSPSASEQNRQFYDQALDGMNDYWRKMAAPRFRVS
                     EFMRLLDEEPFFRLCDLGCGGGQLLREIADRHPRAQLVGVDVAESQLALNRRELPGMR
                     FVGMDLDRPAAVPSDLIDACDVVVASELIEHVSNPQQLLENARGLVRAGGRLLLSTQS
                     GKVRETERRIGHQRHFTVAEMSALLDRAGWQPIRVYNCGFPFHDLSKWYANRDPDASM
                     QQFAGGKYGLREDVLCFLLRLAFRFNSRSHGAQLFAVGRKVG"
     gene            complement(10465..10776)
                     /locus_tag="PKL17_13655"
     CDS             complement(10465..10776)
                     /locus_tag="PKL17_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45828.1"
                     /translation="MKRQNAWFRSLLTVASSIVRCVFSALRFVGQKLVRGGSEALPEF
                     VLSVGMLPSPVRDPAVDAWNELKKRRTQGRNDPPKPSSTSAKVLPIRGSVDDPVIRIT
                     M"
     gene            complement(10871..11557)
                     /locus_tag="PKL17_13660"
     CDS             complement(10871..11557)
                     /locus_tag="PKL17_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45829.1"
                     /translation="MAKDEVDDIEWRLVATKGRESCRWQTSDGRWISLALGAQGDLTK
                     VLVSGSDGRSEVVDGYEQGLNLARRWRASWQRPQEPTSSSQSGSWLPPLPGRAGMPED
                     ISSPDLPPLRHPTAGSNPGSRSRPSVPPTGQSPPTQSSPPASGSFPSRTSLNRVSPVS
                     RGSSTQSNPASSSGNVPSLSGERSALSPTNRDGKRRVSASVPAIRLPDERSSNEYDPS
                     RPSSSSNPKR"
     gene            11668..12657
                     /gene="galE"
                     /locus_tag="PKL17_13665"
     CDS             11668..12657
                     /gene="galE"
                     /locus_tag="PKL17_13665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNK45830.1"
                     /translation="MTVLVTGGAGFVGSHFVRAACEAGRQVVILDDLSGMGVADCDPQ
                     PDIPLPDGAVLVRGDIGDENLVRSICRDHAVSALVHFAGKIQVGESVRKPELYFDVNM
                     ARSLRLIETVRQSGVTDLVFSSTAAVYGEPVTVPIPEHSATVPVNPYGFSKLAFEWVL
                     RSYAVAHGLRFAALRYFNAAGAHPDGTIVERHEPETHLIPLVIDAAWGKRPPLTLFGT
                     DYPTPDGTCVRDYVHVSDLASAHLLALDKLAQGQSLGALNLGTGQGYSVAQVLKAAAD
                     VLGKPVPHTVGPRRAGDPPSLVADSQAAKTRLGWNPQRSDLRTLIEDAARSRR"
     gene            complement(12679..13659)
                     /locus_tag="PKL17_13670"
     CDS             complement(12679..13659)
                     /locus_tag="PKL17_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45831.1"
                     /translation="MTLWRCEETGHGGWSRGLLLLTDEVELWISLEVGPRILRLQRRG
                     GKNLFYEDRTQLGQSGEPSFRLRGGHRFWTAPESTQTYEADNHAVSYQVSGHRVELLA
                     PPFFGLRKTLAIEALTPSLFRVRHGLRNVSAAPISRAAWALSVMAPGGLAILPQPPLR
                     SHPSILPAGTPWRDEDLLPNRRLVLWPYTDLGDPRLRLRGRLWTVEQRCDLGPLKLGL
                     YDQTAGRDVLTGLAAYQLGSSVFVKTVPVCSSQVYPDLGAAFELYTDANFLELETLGP
                     LTTLLPDVTCWHDELWLVRDSAAPLRDEAVGLPFLTDALRCFADHGFPSL"
     gene            complement(13656..14642)
                     /gene="meaB"
                     /locus_tag="PKL17_13675"
     CDS             complement(13656..14642)
                     /gene="meaB"
                     /locus_tag="PKL17_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNK45832.1"
                     /translation="MVDSSQAERIAQIAQAVLSGDLRLGARLIRDLDDQTPLSEAVLR
                     KLWPHTGRAFLIGVTGNPGAGKSTVVDSLIAHYRKLEKRVGVVAIDPSSPFSGGAILG
                     DRIRMQRHALDEGVFIRSLASRGHLGGLSRSTGEVVAVLDAMGFDVVIIETVGVGQGE
                     VDVVALADTKVVVTVPGLGDEVQAIKAGILEIADVLVVNKADREGADRTVRDLLTMLE
                     LRHGEAPLGPEAPSSDVEIVRTVAVRGEGIPELAAAIGRHQVATAHLAANRRARRAKR
                     ELRDALLDRCGLLVDALLARHADWVDEVQRRELDPYSVSERLLDELLQGAPR"
     gene            complement(14668..>15287)
                     /locus_tag="PKL17_13680"
     CDS             complement(14668..>15287)
                     /locus_tag="PKL17_13680"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNK45833.1"
                     /translation="ARSGGDPSGNPRLRSAVDKARGANIPADTIDRAIKKGTGELGAD
                     SVEELVYQAYGPGGVAIVIELATDNRNRTAAELRKLLERQNAKIDPSGSVLHKFKKRG
                     QIIFDAEKHSEDAVTEVALEAGADDVRAEGDTIVVLTEPGACQSVKEAFEKKGMTPLD
                     SEVGMLPELTVALSEKDAEQIGKLVSALEEHDDVMNVYTNHEEAA"
BASE COUNT         3097 a         4765 c         4477 g         2948 t
ORIGIN      
        1 caaatcctca ccgcgctcac ctacctacac cgcagagaga tcattcatcg agacgtgaaa
       61 ccgtctaacg tcctggtgat tccggttccg gagtcgccgg gatttcgggt caagctgctc
      121 gattttggct tggcagtgac ctggcggcgg gtttcgattt ccgcggacac agtggccgga
      181 acattgggat atctggcacc agagcttctg caagggcaga ttcccagtgt ctcttcggac
      241 ctttattccg tgggtgtgct ggcgtatgag cttctggttg ggaagcaccc attcccagtc
      301 gacggaccga cagaacggtt cctgtatcac gtcaagcaca acgcacctga ttttacaggt
      361 ctaaagcccg cggccctgat tcccatcatc acgcaatcgc tggccaagac tccgtgggac
      421 cgtccggaaa gtgcagcgac gtttttgcaa gagctggttg aaaaaggtgg tcttctgtcc
      481 gaaggcgaaa gcgaagcggt acgcgacagt tttctgatcg cggcgccctt tacgggacgg
      541 gaaaacgagt tctgcgcgct ggcgacagag ctggcccagt ctcagcgcgg aatgggggcc
      601 gcgtggttgc ttggtggtca cagcggaatt ggcaagtcgc gacttctcga tgaagtacgc
      661 gcgatggcgc tgattcgtgg tttctgtgtc gcacggggag agacagcgcc gcacggacag
      721 agtccgcttt tgctgtggaa aacaccgctg cgtatgctgt gtttaggaat cagtctttcc
      781 gacgaagaac taagcgctct tgagtgtctg attcctgatg tatccgcact gcaagggaat
      841 ataagtccat ccggtccgaa agtcgttgcg cagcttgaaa ttccgcagct ggccaaggtg
      901 ctgatcgaca ttctgaaacg gcaaccgcag ccggtcttgc tgctgctgga agacctgcag
      961 tgggcggacg aggagtcgct gaccattttg cggactgtgg caagtcatgc ggtgtcgctt
     1021 ccgatgctga tcattgccac ctatcgcagc gaagaagcac cgaccctgcc ggaaaagctt
     1081 cctgagctga gtgtgatgca gctagagcga ctctctgaaa ggtcgctaca gcagctctgc
     1141 acttcgatga tgggagagca agcgtcgtct tctctgcagt ctttgataga acgggagacg
     1201 gctgggaata cctattttct cgtcgagctg atgcgcagtc tggccgaaga agcgggatcg
     1261 ctggatcggg tcgcaggtca cgatctgcta aaagacatcc agcgcggcgg catcatggaa
     1321 gtgttgcgac gtcgcattga gcgcgttccc cagcgagcga tgccgcttct gcgcattgct
     1381 gcggtcgcgg gccgacagct cgatctggcc gtcatcgaac agctgcttcc ccatgcagag
     1441 gaactgattg aagcgtgcgc aagtgtcggc gttctggagc tgcacgatca gcactggcga
     1501 tttagtcatg acaagctgcg ggagtttgtg ctcgccgcac tgccagccga ttcgaagcgt
     1561 gagctgcatt cttggctcgc agagaccatc acgcaggtct atcccgactt gtcgtcgcag
     1621 gcgatgcggg tggcgcatca tcacttggaa gccggacagc ttgcacaggc cggagattgt
     1681 ttttcgctcg ccggatcggt ggcgatttcg gcaggcagtg taaatgaagg cgaacagctc
     1741 ctttcgcgcg ccattgcact gcaaacgcag acgggaagac cgccgatttt gcggctccgt
     1801 acctatcgac agcttgcgct ggtgcgtttc acgctgggaa aaaaccaagg cgccgatacc
     1861 gcaatccagc atgcgtttgc cctggcggga atccgctggc cacacggagt aggagcgcgc
     1921 acgatcgata cagtggcacg atttgtagaa cagacgctgc ggcaaagcag acacctgaaa
     1981 gcgacggtac agcatgccga agaaaaggaa ctgtatagcg aactcctaaa gattctgccc
     2041 gctaccgaag tgtatcttgc gctggcacag cctgcgtcgg cgatgttctg ctgggtgcac
     2101 tgcttgcgga ttgcggaaac ggtggatgat gtgatggcgc acctgcaagc tgcgtcgttt
     2161 gtggcctatg cattttcgtc ggtgggactg cacacactca gcgcgcagta tctggcgcgc
     2221 agcgaatccc tttcgcaacg ctcgcagcaa gccgaagcga tgatcttgat cgagcgcgcc
     2281 aaagcaggaa tcgcgcttgc agcaggacgc tttggcgatg caatcagcgc atgtcagcac
     2341 gcgcgtcagc agaacacgac cgcacaggaa ctgaccccca cctttcgcat tctgcgagtc
     2401 gagctgctcg cgcacttgct cagcggcaat ctgtcttcgg cctggtctct tgccgacgag
     2461 ctatgggcgc tttcggaacg gcacgatctc ccgcagaaac agacgctggc ggtcggctat
     2521 cgcggtgcgc tgtgggcgcg gcaaggagat cccgtgcggg ccgcacgatt cctcgaacaa
     2581 gcgagtggtg cagataccgc gctggaggat tcccatcacg cgctgtggtt tggtggccag
     2641 ctggcatcct gctatgcgaa acacggtcag ccgcaccgcg cactgcgtct tgccaagacc
     2701 caagtacagt ctctccgctc gcacagcgtt gcatccatct tgagcgctct tgggatgtac
     2761 gcgctcattg aaacgtactt tgcgctcggc atggaatcgg cgtgcagtgt gtcggaagcc
     2821 agcgcagaag tgtcgacgat catgaaacag ttgggtcagc ttgcgctgcg ctaccgtcct
     2881 gtttccccga ttttcgagct ggggcttttt cacatgcttc agctcaaagg acaggtggcg
     2941 ctttcgatct gggctctgcg acgagcgctc gtgcatgcca gcgccctttg catgccgtat
     3001 gaagaagcgc tcgcccacac gttcttggcg gaacaagctg aaaaggcatc cccagcggtg
     3061 ctgctgctga ttccggaaag tccacagacc caccgcgccc gcgcccaaga acgattcgag
     3121 cagctcggaa tccccatccc caggcaatca ccgacgtaaa atccgtcgac aaaccagaat
     3181 gcccgcgcgg actgtgggct tcggattttg cacagcgaaa accgatggca agcgcacacc
     3241 gttcgcagta cggttcgctt ctcatgtcgg caatacatgt tgatgtgcat ccaatcggaa
     3301 gtcagccgcg cccacgcaca aggagtcttc cgtttgcaaa gctggcgctc tgtgttttcg
     3361 tcgcaggact tcacgctgcg ggctgttcat cgggcggcta ccggccccct gtggatgaca
     3421 aacacggaac agatggtggc gtgtccgatc ttgcgatcac ggaaccgggc cgtgacggcg
     3481 gaaccctgtc ggatggcggc atgggtagct gggatcttct tcccgatctg acggcaccac
     3541 caagcagcga tccattcgat ccagcaagct gccccggccc ggcgtggtcc gcagccagtg
     3601 cactgacaac actcggcacc gcggcccgcg caaagcttgc ggatgcgacg ttgatgcgac
     3661 gaacccgcac ttgcacggga acaacgccag ccacctgcgg cgcgtggtcg acaccgattc
     3721 cccacaccca ggcgctactg acgtactccg gtggcgtgac gacggactac aagacgtttt
     3781 cgttcccaac ccatctcatc ctgtttgcac agtccggaca gcccaagctg gtggtccgtc
     3841 acgaatcaga ctaccgacac gatgcaactg caaacacccg cggcgtggtc tttccgcttg
     3901 gcgcgttccc gattgaaaac acctatccgg tgatttacgt gtgggacttt gcgccggctc
     3961 cgtaccgtta cgatgatctg caaggactgc tgggaggcaa aggccagctt ttcgctgccg
     4021 aacgctgcgc ccgcgcggta ttcaccgacg gactcacgac cgaaattgcc gcgctttatc
     4081 gctactaaga acaccgcgac aatgcacagg cttaggtctc tggttcgagc gtacagagca
     4141 gcggatattg cgcttcccgc gcgagcgcca tcactttggc gactttcgat tccgccactt
     4201 catgggtaaa cacgccagcg acgccaaccc cttggtgatg gatgtgcaac atgatcttca
     4261 tcgccgccgc attcgagtga tgaaagaccg tttccagcac cgcgacgaca aactccatcg
     4321 tggtgtagtc atcgttgtga agcagcactt tgtacatacg cggtggcttg accttgggaa
     4381 cgactttccg gtccaagacc acattggtgt cgtcgtgctg atgccgctct ttgctcatgg
     4441 gatctcattt tggtttacgc gaaaacccaa gccgcaacaa gggccgcttg ctaaaagcaa
     4501 aaaatgagta tgctccggcg catgcgacga cacctttggg ttgcggcgac ctgcgccgcg
     4561 ttgtcatttg gttgtgagca gatgccaccc gacgtcgagc cgaaagccag cttcagcgtt
     4621 cgcgaaagca tcggccagct tgccgtcacc cacgccccac ccggcgccac cttggatctc
     4681 cgcgatgaag gcggaaatct cgtcggaagc ggtgttgcgg acgagctagg cagctatctg
     4741 ttccgcggcg tcaaacccgg cgcaggctat gtgctcagcc ggcgcggtgc cgatcccgaa
     4801 gatcgcaccg gcaaactgac ggtgtattcc gtcgaatcaa gccgcaaacc ccagtcgttt
     4861 tatgccaacc aaaaactgtc gcccggtttc ggttacatca cgacccgcga cggcacccag
     4921 ctttcgattt acgtcacctt gcccggaccg atcgaaaaag ggccgtatcc gacggtggtg
     4981 aacatctcgg ggtattcgcc ttcgcagccg ggcaaacagg acgacaagta cgccgggctt
     5041 tgcatcgcat atccagcgct gtgcgatcag cccaacgatc cgagcgcgat gatcgcagca
     5101 gtaaatggct atgccactgt cggtgtgaac ctgcgcggca cgggctgttc tggtggcgcc
     5161 tacgactttt tcgacacgct gcaaaagctg gattcttacg acgtggtcga aaccgtcgcg
     5221 gcgcaagact ttgtgctgca ccacaaagtg ggcttgaccg gactttccta tccgggcata
     5281 agccagcttt ttaccggggc gcagcgtcca aaaagcctgg ccgccatcac accgctgtcg
     5341 gtgatcggca acatgtttat gacgctggca ccgggcggcg ttctcaacag cggcttcgcg
     5401 ctcagctgga tcacccgcgt gctcgacaaa gccggtccgt acaagcaagg ctgggaacaa
     5461 ggcgtcgtcg acctgggcga ctccatctgc aaagaaaatc agcttctgca cggacagaaa
     5521 gccaacttga tcgatgtgat caaggcgcat cagtactatc cgccggagct gggcgacgcg
     5581 gtcaatccgg aaacctttgt cggagacatt gatgtgccgg tgttcctggc tggcgcgtgg
     5641 caggacgaac agaccggccc gtattttgtg tcgctgctca atcgcttccg cagtgcgccg
     5701 ctcacgcggt tccatatgta caacggcgtg catcccgacg gctttgcacc gcagattctg
     5761 atcgagtgga agacgttcct cgatctgtat gtcgcgcgca aagtgccgca ggtcgatcag
     5821 ccgctgcgcg tgctggtgcc gcagctgttc aaagacttct tcggcgccaa aatggagcta
     5881 ccgcccgatc ggttcgccga tcagccaaat tgggaagccg cgcgcaaaaa gtacgaagcg
     5941 gagcccgccg ttcgcgtgct gtttgagtcc ggcggcgtat cgaccaacct gggcgctccc
     6001 gagtcacggt tccaagcgac gttcccggct tggccgccca agacgcagcc tgtgcgctac
     6061 tacttccgcc aagatggcag cttgtcgacc agcactccga cggaaaccag cgcagcgacg
     6121 cagatcgtgt tcgatcccaa tgccggaacc caaggcaatc tcgcaccggg cggcgacatc
     6181 tgggcgctga ttcccaagtg ggactggaag ccgctgcaaa ccaacaaagc cgccgcgtgg
     6241 gaaaccgccg cgctcagcga agacgtggtg atggtcggca ccgcgagcgt ggatctgtat
     6301 gtgcaatcga cggcaaacga cgcggacatc caggtcgtgc tcagcgaaat ccgtcccgat
     6361 ggaaaggagc agtttgtgca agctggcacg ctgcgagtca gcaaccgcgc gctgacccgg
     6421 gatgcaacgc cgctgtggcc cgaacattcc tatcgcgaaa ccgatgcgac accgctgccg
     6481 gctggaaaat ggagtgaagc gcgggttggg attcctggct ttgcacacgc tttccgcaaa
     6541 ggctcgcgac ttcggctgtc ggtgggcacg cccggtggca accatgccga gtggcgattc
     6601 caaacactgc cccaatcacc gacgacgcag caccagattg cgcacagcgc tgcccagccg
     6661 tcgagcattc tgcttccggt ggtttctggc gtgacggtgc caccccagct tgcgccctgc
     6721 ccttcgttgc gcggtcaacc ttgccgcagc taccaagcgc tggcgaacac cccggccccc
     6781 taacagccgt cgctccgccc cgtctgcaag ggcggagccg cttccccttt acccgccgaa
     6841 aatccagctt cctaacgccg tccaatccct tggtagacaa gcccggcggc ccgcacctgt
     6901 gccgggtccc aaatgttccg cccgtcgagg agcagcttgc cgcgcagaag ccgcacgatc
     6961 ttgcgaaagt ccggctggcg aagctgtcgc cactccgtac acagacacaa agcgtccgca
     7021 tccggcagcg cgtcataggc cgacgccacg atttcgacct gtgacatttt ttctttgagc
     7081 gtcgccgaaa gactctggcg ggccgtctcg ctcgcgaccg gatcgtaggc acgcacttcg
     7141 gcacccagcg caaccagctt ttcgataagc gtcagcgccg gagcctcgcg cacatcgtcg
     7201 gtttcgggct taaacgaaag accccagacc gcaatccgct tgcccgtaag cgccagcggc
     7261 tcatcttccc gcttgccaaa atgcgcgagc agcttgtcaa aaagacgccg cttctgccgc
     7321 gcgttgatcg attccgtcgc tgacacgagc ggcagcggca gaccaacatc ttcagctatg
     7381 tgaatcagcg cgcgggtgtc tttgggaaaa caggagccgc catagccgac gccaggaaac
     7441 aggaagctcg ggccaatccg ctgatccatt cccatgccgc ggcgcaccag ctccacgtca
     7501 gcgccgatgt gttcacacag ccgcgccacg tcgttcataa acgagatccg catcgccaga
     7561 tacgcattgc tcgcgtactt ggtaagttcc gccgatcgcg cgtccataaa cagcaagcga
     7621 tcgtttttgc gcaccaccgc ggaatagagc tgccggatca ccacgcgggc ccgttcatcg
     7681 acttgctcgg cagtcgaaag cgccggcgag cgaccagcag gaccggcact tggaccgacg
     7741 cccacgatga cgcgatcggg acgcaaaaaa tcgtcgagcg cgctgccttc tttcagaaat
     7801 tcgggattcg atacgactgc gaacggtctt tgcgaaaccc cagcaatgat gtcgcgcaca
     7861 cgctccgccg taccgacggg aacggtggat ttgtcgacga tcacggtaaa ttccgacgac
     7921 agatgtttgc ccaccgcttc tgccgccgcg aacacctgcg acaaatcggc tcgcccgtcc
     7981 gccgcaggtg gcgtcccaac gcagagcaag atcacatccg cgtcttcggt ggcgtcggca
     8041 aacttcgtcg aaaaacgaag tctcccggca gcggcatttt cagccaccat cgccgggaga
     8101 cccggttcgt gaatgggaac gtcgccgcga agcaaacgag caatgcgctg ctcatcgatg
     8161 tcgatacaga ccacgtcgtt gccaaagtcg gcaaagccgg cacctgccac caaaccgaca
     8221 tagcccgtac caatgactcc tattcgcatc gtcttccttt gcccgagctt acgcggcggc
     8281 gcgcctcact acggcagctt cggcgggtcc gccgtgtagg cgctgccgaa ctcttgcacg
     8341 tagcgatacg agatccagcc gtagccagga accttggaat tgtacaaacc aaagtttgcg
     8401 gttccccagc tgtttttgaa aagataaaag cccttctgct tgatcggctt gcccgctgca
     8461 tccaccgccg gacgaccgtc tttgtccatc gcctggattt ccaaatcgtc gtcgtagccc
     8521 acaagctgca ccgagtgacc cgccggctgc ttcgccgacg cggcctggtc ttccgcattt
     8581 ggatacagca cgtagccttt ttggaaatac gccgagctga ccggcagcgc cgaccgacgg
     8641 tgattccagg cctgatagaa gaagtcgagc ccgacaacca cgccctgctt cttttcaaag
     8701 atgatgttct tgatcgagct ggtcgacaca tagcggcccg ccgacggcag cttgtacttg
     8761 gtcgcactgc tcgccgcagc cggaggatcg ccgtttgtgt agcagatgtt gggcagaccg
     8821 tccccggttc ccgtgcagcg cgagtctttg gtgacatccc actgcgtcgg ctcgtatggc
     8881 cacaccgttt cagcgacgac accataggtg ttcaccgcct tcaggttgta gtagtcgctt
     8941 gatccagaac tgcccgggaa ggcgtttagc ttggctttga cgagccactg aagatactgc
     9001 tcggaaaagt ccggcgtggt cgtcatcccc gcttttttga acagcgattc gaccagaccc
     9061 agcgacgaaa agatcgagca gacgccccgc gaggcctggt ttttcaccga agtctggatc
     9121 gaaagcacgt cggcctgctt gggcagcacg acgtcggcct tgccctcgaa tggaatcgag
     9181 gaatccgacg gtgcatcggc cttgagcacg cccaccgaag tcagtggcgc atcgttgcct
     9241 tcgacttcgc tcggcgaaac cggcgtccat tcatcggacg atgccggttc gcaaccgatc
     9301 agcaaagaaa agcccagcac cgacgaggca aacagcgccg aacgaacgac agacagacgg
     9361 gaaacagggg tacgcatcgc aaaactcctt ctctgtgggt tgcactggcg ggttcggttt
     9421 ggatgcttgc gtcgccgcca ggcaaagtgc gcggcaccat accaggcttt gaatgcgaag
     9481 caagccgccc ataaaccgac ccgaaagcct gcacaccata gccaaggggt ctgacaagcc
     9541 ctgcgcaaag ggccaaacgg gattatccga ctttgcgacc gacggcgaag agctgcgccc
     9601 cgtgggagcg cgagttgaaa cgaaaggcga gccgcaaaag gaagcacagc acgtcttcac
     9661 gcagaccgta tttgccgccg gcaaactgct gcatcgaagc gtcggggtcg cgatttgcgt
     9721 accacttgga caggtcgtga aacggaaaac cgcagttgta gacgcgaatc ggctgccagc
     9781 cggcccgatc caaaagcgcg gacatttcag cgacggtaaa gtgacgctga tggccgatgc
     9841 gtcgctcggt ctcgcggact ttgccactct gcgtcgacaa aagcagccga ccgcccgcgc
     9901 gaacaagtcc gcgcgcattt tccagaagct gctgcggatt gctgacatgc tcgataagtt
     9961 ccgacgccac gaccacatca caagcgtcga ttaggtcact tggcaccgca gcgggacgat
    10021 cgagatccat cccaacaaag cgcatccccg gcagttcccg ccgattcagt gcgagctggg
    10081 attcggccac atcgacgccc acgagctgcg cccgcggatg gcgatcggca atctcgcgca
    10141 gaagctgtcc cccgccacaa ccaaggtcac acagccggaa aaacggctct tcgtcgagca
    10201 gacgcataaa ctcgctcacc cggaagcgcg gcgctgccat tttgcgccag tagtcgttca
    10261 ttccgtcgag cgcttggtca taaaattgac gattctgctc gctcgccgac ggtgaggaat
    10321 cagcggtagg tttttgcggc tgcatgggaa gatgcccatc ttataccacg gcccgacgcc
    10381 aaggcgccgc agctaccttg ggtctttgtc gacggaaaag taatcgggaa aattgggcaa
    10441 ggcggggatt gcaaaaacga gcggttacat cgtgatccga atgacgggat cgtcgacgct
    10501 gccgcgaatc ggcagaactt tggcagatgt cgacgacggc ttgggtgggt cgttccggcc
    10561 ttgggttcgg cgctttttca gttcattcca tgcgtcgacg gcaggatcac gaaccggcga
    10621 aggcaacatt ccgacgctga gcacaaactc gggcagggct tcactgccac cgcgcacaag
    10681 cttttgtccg acgaaacgca gcgccgaaaa aacgcaacga acgatcgagc ttgcgacggt
    10741 gagcagtgaa cggaaccaag cattttgacg cttcatatcc agaccctttc ctgtcgttgg
    10801 gaacggatat tgtggcgcat gggtctgaca cccattttcc ggcctaattt cgcagatttt
    10861 ccgtcgacaa ttagcgtttg ggattgctgc tagagctggg gcgacttggg tcgtattcgt
    10921 tcgaggagcg ctcgtcgggg agtcgaatgg caggaacgga agcgctcacc cgtcgtttcc
    10981 cgtcgcggtt cgtcggggaa agcgccgagc gctcgccgct caaagatggc acgttgccgc
    11041 tgctgctcgc tgggttgctc tgcgtgctgc tgccacgact gaccggcgaa acacgattga
    11101 ggctggttcg gctcgggaac gaacccgaag ccggcggcga cgactgcgtt gggggagact
    11161 ggcccgtcgg gggtacggat ggacgactgc gcgaaccggg attcgatcct gccgtcggat
    11221 ggcgcagcgg tggcaggtcc ggtgatgaaa tgtcttcggg catcccagcg cgcccaggca
    11281 gcggcggcaa ccacgatccc gactgcgacg aagaagtcgg ttcctgtggt ctttgccagc
    11341 tggcgcgcca gcgacgggca aggtttagac cctgttcata tccgtcgacc acttcgctgc
    11401 gcccgtcgga accgctgacc agcactttgg tcaggtcgcc ttgggccccg agtgcgagcg
    11461 agatccatcg accgtcagac gtctgccaac gacagctctc tctgcctttg gtcgcgacga
    11521 gacgccactc gatgtcatcg acttcgtctt tggccacaca gaaatcgtat cacagaacgc
    11581 gctttgccca tctgccgaaa tcagcagtgc tttttcccgg tcaatctcca gcggttccgc
    11641 tgcagccgct cagctaagat gcgtcccatg acagtgctgg tgactggcgg tgcgggcttt
    11701 gtcggttccc actttgtgcg tgcggcttgc gaggcagggc gccaggtggt gatcttggac
    11761 gacttgtcgg ggatgggcgt cgcggactgc gatccgcagc cagacatccc acttccagac
    11821 ggcgccgtgc tggttcgtgg cgacattggc gacgaaaatt tggtgcgatc gatctgccgc
    11881 gatcacgccg tttcggcgct ggttcatttt gccggcaaga ttcaagtcgg cgaatcggtg
    11941 cgaaaaccgg agctctattt cgacgtcaac atggcccggt cgctgcgtct tatcgaaacg
    12001 gtgcgacaat caggcgtgac cgatctggtg ttttcgtcga cggcagccgt ctacggcgag
    12061 ccggttacag tgccgattcc cgaacattct gcgacggtgc cggtcaatcc atacggcttt
    12121 tcaaagctcg cgttcgagtg ggtgctgcgc agctatgccg tcgcccacgg actgcgcttt
    12181 gctgccttgc gctatttcaa cgcagccggc gcgcatcccg atggaacgat cgtcgaacgg
    12241 catgagccgg aaacccactt gattccgctt gtgatcgacg ctgcgtgggg gaaaagaccg
    12301 ccgctgacgc tgtttgggac agactacccg acgcccgacg gaacctgtgt gcgcgactat
    12361 gttcacgtct cggatttggc ttcggcacac cttctcgcgc tcgacaagct ggcgcaagga
    12421 cagtcgctgg gcgcgctgaa tctgggcacc ggccaaggct acagcgtcgc gcaggtgctg
    12481 aaagccgctg ccgacgtatt gggcaagcca gtgccccata ccgtgggtcc gcgccgcgct
    12541 ggagacccac catcccttgt cgccgattcg caagccgcca agacccggct cggctggaac
    12601 ccgcagcgtt ccgacctgcg gacactgatt gaagatgcgg ctcgttcgcg acgctaatca
    12661 agcgacacgg ggtcgcagct acaaagacgg aaaaccgtga tccgcaaaac agcgcagcgc
    12721 atcggtcaaa aacggaagtc ccaccgcttc gtcgcgcagc ggcgccgctg aatcgcgaac
    12781 cagccacagc tcatcatgcc agcaggtgac atccggtagc agcgtcgtaa gcggccccag
    12841 cgtctctaat tccagaaaat ttgcgtcggt gtaaagctca aacgcggcgc ccaggtcggg
    12901 atagacttgc gacgagcaca ccggcacggt ctttacaaac accgacgagc caagctgata
    12961 tgcagccagg cctgtcagca catcgcggcc agctgtctgg tcataaagac ccagcttgag
    13021 cggcccaaga tcacagcgct gctcgacagt ccacagccgt cctcgaagcc gaagccgcgg
    13081 gtcgcccagg tcggtatacg gccacagcac aagccgacga ttgggcaaaa gatcctcatc
    13141 gcgccacggc gtccccgcag gcaggatgct cggatgcgag cgcagcggcg gctgcggcaa
    13201 aatcgcaagc ccacccggcg ccatcaccga cagcgcccac gcggcccgcg aaatcggcgc
    13261 cgcggacaca ttgcgcagac cgtggcggac gcggaacagc gatggtgtca gcgcttcgat
    13321 ggccagcgtc ttgcgcaggc caaaaaacgg cggtgccaac agttcgacgc ggtggccgct
    13381 gacttgatag ctcacggcgt ggttgtccgc ttcgtaggtc tgcgtcgatt caggcgcagt
    13441 ccaaaagcga tgtccgccgc gcagacgaaa cgatggctcg ccggactggc caagctgcgt
    13501 tctgtcttcg tagaagagat tttttccgcc ccgcctctgc aaacgcaaaa tccgcggccc
    13561 gacttcgagg ctgatccaca gctccacttc atcggtcagg agcagcaagc cacgggacca
    13621 gccgccatgg ccggtttcct cgcagcgcca cagcgtcacc ttggcgcccc ttgcaaaagc
    13681 tcgtcgagca ggcgttccga cacactgtac gggtccagct cgcgccgctg tacttcatcg
    13741 acccaatcgg catggcgcgc aagcagcgca tcgaccaaaa gtccgcagcg atcgagcagc
    13801 gcatcccgca gttcccgttt ggcccgtcgt gcccgtcggt tcgcagcaag atgcgcggtc
    13861 gccacttgat gacggccaat cgccgcggcc agctcgggaa tgccttcacc acgcaccgcg
    13921 accgtgcgga caatttcgac atcgctcgac ggcgcttctg gtccgagcgg cgcttcgcca
    13981 tggcgcagct cgagcatcgt cagaagatcc cgcaccgttc gatctgcacc ttcgcgatcg
    14041 gctttgttta cgacgagcac atcggcgatt tccaagatcc cggctttgat cgcctgcact
    14101 tcatcgccaa gccctggcac cgtcaccacg accttggtat cggcgagcgc aaccacgtcc
    14161 acttcacctt ggccgacgcc caccgtttcg atgatgacga catcaaaacc catcgcatcg
    14221 agcaccgcaa ccacttcacc cgtcgagcgc gacagtccgc ccaggtgtcc acgcgacgcc
    14281 agcgatcgaa tgaacacacc ttcgtcgagc gcgtgacgct gcatgcgaat ccgatcaccg
    14341 aggatcgcac caccggaaaa cgggctcgac ggatcaatcg caaccacgcc gacccgtttt
    14401 tccagtttgc ggtagtgggc aatcaggctg tccaccaccg tcgatttgcc agcgccagga
    14461 tttccggtca caccgatgag aaatgcccgt ccagtatgcg gccacagctt gcgcagcacc
    14521 gcttccgaaa gcggcgtctg gtcgtcgaga tcgcgaatga gccgcgcccc cagccgcaga
    14581 tcccccgaaa gcaccgcttg cgcgatctgc gcgatacgct cggcttgact cgaatcgacc
    14641 atgatagacc gcctacagcg acggcgacta ggccgcttcc tcgtgattgg tgtagacgtt
    14701 catcacgtcg tcgtgctctt cgagagcgga caccagcttg ccaatctgtt cggcgtcttt
    14761 ttccgacagc gcgacggtca gctcgggcag catgccgact tcagaatcga gcggcgtcat
    14821 gcctttcttc tcgaacgctt ccttgacgct ctggcaagcg cctggctcgg tcagcaccac
    14881 aatcgtgtca ccttcggcac gcacgtcgtc ggcgccagct tcgagcgcaa cttccgtgac
    14941 ggcgtcttcg ctgtgctttt ccgcgtcgaa aatgatctga ccgcgctttt tgaatttgtg
    15001 caaaaccgat cctgacggat cgatcttggc gttctgacgc tccagcagct tgcgcagctc
    15061 ggcagcggtg cggttgcggt tgtccgtcgc cagttcgatg acgatggcaa cgccgcccgg
    15121 tccgtaggct tgatagacca gctcttcgac ggaatcggcg ccgagttcgc cggtgccttt
    15181 tttgatcgcg cgatcgatgg tgtcggcggg aatgttggca ccgcgcgctt tgtcgacggc
    15241 gctgcgcaga cgcggattgc ccgacggatc accgccgctg cgggcag
//
LOCUS       DAORGN010000123        15125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1627_length_15125_cov_5.239615, whole genome shotgun sequence.
ACCESSION   DAORGN010000123 DAORGN010000000
VERSION     DAORGN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 15125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15125
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_15125_cov_5.239615"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKL17_13685"
     CDS             <1..363
                     /locus_tag="PKL17_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45834.1"
                     /translation="FVYVDKGSMATIGRAAAIAHIGRLNIGGFLAWMLWLLVHIFFLI
                     TFRNRIAVLFNWAYQYFTYKRGARLITGHRLKAGAESDVAVTALPKSALTAALLPPEA
                     AKTTPAAPPETAPPAVPR"
     gene            complement(278..802)
                     /locus_tag="PKL17_13690"
     CDS             complement(278..802)
                     /locus_tag="PKL17_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45835.1"
                     /translation="MPAVELLSCLHRLDLSSHSAVPFGRPGLLLALLGEPGTAHAGLA
                     ELLIELKAESLVASHLALSSTNLLSGLVASCLGDTPVGATVVLPSALCVGDPTGIRLL
                     GDAGHHHLLGIWADRQADARLRAQAHHVPLWPLGRTTGQELLIRLSEGPQAEPFPVVL
                     RASFSQLQAEVVRR"
     gene            865..1803
                     /locus_tag="PKL17_13695"
     CDS             865..1803
                     /locus_tag="PKL17_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45836.1"
                     /translation="MAEPLLSVKSLRVDYEDITAVDDVSFEVEAGMVYGLIGPNGAGK
                     TSIIRVIATLLQPTYGQVRLCDIDLLGDPLRALSHLGFMPDMPPLYDDLYVEEFLQLF
                     ASAYGLFGAAQTKRIDELLDKVQLSEKRKTPAGELSRGMKQRLFLAKTLLHDPKVLLL
                     DEPASGLDPRARLDLRDVIVELGRAGKAVVVSSHILAEMADFCNSVGIMERGKLLLSG
                     RIDDILKQLRPGLLIRVRLLSPDDRIGPLLGGSPGVSEVETDGAQSTFKLAGDGEAAA
                     NLLRDLIQKDFRVAEYREMTSNLQDIFLQVSSGRVS"
     gene            1800..3356
                     /locus_tag="PKL17_13700"
     CDS             1800..3356
                     /locus_tag="PKL17_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45837.1"
                     /translation="MSEPVVRLVRNPILIAQARRRLRPAQLLSSTLIFGLLGICGVLL
                     SVVAKDRHEAFSSFEYVLLTGIGGLLLLRGSNRVAETVREERESGILDFHRATPTTPW
                     TDGIGYLLGCPAREYLMAGILAGFALACATASQFGLLPVLGSLVAIGTSGLLYHCFAL
                     WIGLSVAHKRGASGIVMGTLVVLLIGGWSARGVGAVSYFTPYPALFWLLSSKEAKITE
                     VTFYGLPMHALVLTLLVQGSVLLAMGTAVARKLRQDDATSFSRLGTLAFFAWIAFLSL
                     GAAWQWISPASGGPLSVTASTDTAVIAGYLLVSTIAAAALLIGQVPSYLSLLRALRRA
                     RRLKSATISWLDEGGRCEPLTLGLWGLTVAGLLAIHHAMGVTRVSGPLLGTPLLLAAA
                     AALCYLLFVQTATEFVRIRHRGATSAVAVFVFFASHVLPFLLSFVFGASKATPGLLVM
                     AASPIYGVWAAAAHLTEAWLPSVQDKTEPLSIYVSLAVSLAVSAFFFIESRRSQADLA
                     RKIADSTKPS"
     gene            3363..4304
                     /locus_tag="PKL17_13705"
     CDS             3363..4304
                     /locus_tag="PKL17_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNK45838.1"
                     /translation="MTATSAPGLSAKSGRTIGFAFVLGAALSWSLAGIGIKSVALPGL
                     AIVGLRSLLALPVFALVLWLRAGQNLALVLRLGLGRPITWLASACYAWTMLSFVLAAK
                     LTTAANAILLQYTAPIYVVLLSWPLLGEKIGVADVLAVLGCFLGIAWFFVEQLSTSGL
                     YGNLLALSSGAGLGLLPLLLRRTERPDPSHDAQSRELLPFVPTLVLLLGNVLAVLAGL
                     AAMLRQPVPGLSAWLWLAVLGVVQIALAYLLYAAAVRRLRPVECLMLASLEPILNPIW
                     VALFAGELPSRAAICGGLLILASVTGHGIYSALRPRH"
     gene            4342..4893
                     /locus_tag="PKL17_13710"
     CDS             4342..4893
                     /locus_tag="PKL17_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNK45839.1"
                     /translation="MSLVNDEPAPSPEILARLRPIRLLCLDVDGVLSDGHLYFAGGEG
                     DARFCQRFSVRDGVGIRLLMQSGRHVAILSEGNILSGRVRAESLSIRHAYFGLRDKVA
                     KFSELLSELGLSKSQAAFIGDEVSDLPLLHEVGFAATVPDAVWEVRRAVHHVTTRPGG
                     SGAVREVCDLIRRFGDTTPAGTI"
     gene            4893..6089
                     /locus_tag="PKL17_13715"
     CDS             4893..6089
                     /locus_tag="PKL17_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK45840.1"
                     /translation="MSSAVLLTGATGFLGRDLLRKFVEELPPEQTVYCLIRGHAQGKD
                     KDQHLSPQQLADKRLHTLLSELGFHDRSDPRLRRVSAIAGDISQDGLGLSQAAYAELA
                     AQVEHIYHGAATVRFDQPLAEARRINVEGTRQILDLALLAKRSGRAVRLSYIGTSFVA
                     GTRRGRIYEDELVVDQVFHNTYEQTKAEAEALVQKAIAEHGLRATLFRPSIIVGDSRT
                     GETSSFKVMYWPLKIFSRGLIPIVPASRAGVVDMVPVDYVISAIWELSRRTDTVGGCY
                     HLAAGPEGSTTIGEALDQAAAFFRVRKPLFVPTETFERYIRPLFHLFFRGKRRQALDA
                     GRVYVPYLNYQASFDTEKTRTALRGTGIAPPSVRDYFAKLMRFCVDSDWGKRTIHPSA
                     PRRPGQ"
     gene            complement(6063..6782)
                     /locus_tag="PKL17_13720"
     CDS             complement(6063..6782)
                     /locus_tag="PKL17_13720"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK45841.1"
                     /translation="MPDTERLSRICTELASDPRYAGGSKRFALAISDAEAMSIAEALA
                     AELDEGVAARAAQAARQGFHIYCQKGCTACCAVLVVSYLPEALRIAAYLNQPEHADAK
                     AAFLAAYPRWKEQAGNVPERATAAFVEGRQAEYDALHLEHFRRAIPCAFLVDGACSIY
                     PVRPLGCRNAHALDTADQCVADPPGGRPPAAVDFVPLTRLIHRATQLLHAVHNVKSRQ
                     RHGQQSVCVAVYELLTRPARR"
     gene            complement(6786..8948)
                     /locus_tag="PKL17_13725"
     CDS             complement(6786..8948)
                     /locus_tag="PKL17_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNK45842.1"
                     /translation="MSPALLVPSAGQSHKIMGRVLGVLVALLLSHFALPVRADEGMWT
                     LTDFPRERISRKYGFQVTDEWLKKVQLASLRLANGCSGAFVSADGLVMSNHHCVGDCI
                     VEQSSAQRDLSRNGYYAPSHASELKCSSLEFHQLLDIEDVTSRLQAATKGLSDAEFGV
                     VLRREISRLEQDCASATKLRCDVVTLYHGGKYHLYKYRRYSDIRLVFLPETSTARFGG
                     DPDNFNYPRYSLDVSFVRVYDNGQPLKTPNFFPVNPVGVTPGELVFSTGNPGSTQRLM
                     TVSQLEFLRDVANPELMLYLAELRGHMLEFAKLGPENRRIMIEPLYGVENWYKALVGQ
                     QETLTSRNFLSAKRSSEEALRSQVAQNPDWQRRYGGAWDALSRAQDELRKIYKEHTLL
                     EVGRAFNSTLFHYARQLVRAAEERPKPSDQRLSEYRDSALPSLSHELLARSTMYPQLE
                     ILTLTYSLGRLREALGPNHPFVKETFGPYSPEEIARNLIMGSRLADPAVRKTLWDGGQ
                     KAIAASTDPMIRFAKAVDSQSRLIRRQYQDSVESVEKRNSELVAQALFAVQGTSVYPD
                     ATFSLRVTYGTVRGYKEGSTELSPITLLGGAFDRHTGREPFVLPPSWLAAKGRLKAST
                     PLNFVSTLDIIGGNSGSPVLNRDAQVVGTIFDGNIPSLGGAFFYDDTANRAVSVHTSG
                     LFEVLRGVYNAGPLLKELQSANTQAAVKTPPAQAPAPK"
     gene            complement(9076..10470)
                     /locus_tag="PKL17_13730"
     CDS             complement(9076..10470)
                     /locus_tag="PKL17_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNK45843.1"
                     /translation="MTIQRSKVSSFFVGSLICGQLLALTMIGCEPPEPVTGDDTVMPP
                     PPPPPPPPPSEDPDCPRELKCEDIPAAYKARSATPGDYGNYDLANRPKDGMPIRYIVI
                     HTTEGAWDGVVSHFQNPTASAAAHYLVGSTNGRVAKFVAPSHVAWHAGNWYFNMHSIG
                     IEHEAVAIEGKKWFTDAMYENSAKLVRHLAKSYGVPIDREHIIGHDEIPGVTAAKQGG
                     MHWDPGPYWDWDRYMQLLLAPLPSEKDSGKDGDALVLRPGYDTNLQKGSYCYGSMPSD
                     CKDVPEAPANFVYLRTAPDLSAPLIENPYLKGVPGDRMNNWGDKLGTGPMYVRAEQKG
                     DWDAIYFGGQKAWFLNPGRSLTRKAATMMVTPKSGLSTAPVYGVAYPQDAAYMPPTKP
                     AVMEKIYDVPSGHRYALAARATGDYYWAKEFVSAYDPSKQIVVKDGTEYYQIHYNHRV
                     AWVRAADFELVTAP"
     gene            complement(10513..11988)
                     /locus_tag="PKL17_13735"
     CDS             complement(10513..11988)
                     /locus_tag="PKL17_13735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45844.1"
                     /translation="MLQCAECLTRYADGTHACPLDGGALLTVAPAGDYGHGVTPHLSV
                     QAPIGRSSIGPDSADADDTLVGEVLGGKLKVRRRIGQGGMGTVYVAEHLHLHKQVAVK
                     VLNAQLALTEDLVSRLHSEARLASSISSPHIVEVFDVGLTADGRPYVEMELLVGESLA
                     QRLARCSALSEVDTLRIGEQLATALSAAHESGVLHRDIKPENIFLCARDDGDFVKLLD
                     FGIAKAVRINDEPRLTRTGAILGTPLYMSPEQVRGDALDPRADVYGLGVVLYECLTGS
                     VPHCAASYLSVVAKILTESAEPPSSRCPDRQISAGLESIVMHAMARDRDLRYGTMAEL
                     LADLRRFRDGLPPLALPHAVPTSPAMDPGTLSNTLRQAMFFGALCLLLVGLFGSRWWV
                     QRPILAPPVVAPRPVPPSSDPLGPAPAGAPLPLPPSPALAAQPAQHQVDRIEPKRAPV
                     LSRRGGRSIAVTQQKTAEADPLGEEQAPNPFVGKPDLGRGR"
     gene            complement(12001..13044)
                     /locus_tag="PKL17_13740"
     CDS             complement(12001..13044)
                     /locus_tag="PKL17_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNK45845.1"
                     /translation="MLTLLVLVIAFALIFDYINGFHDAANAIATVVSTGVLPVRTAVL
                     MAAVLDFTGALTGTAVAKTIAAGFTDPKMVSQVVVLAALSGACFWNLFTWWFGIPSSS
                     SHALIGSLAGAVVAKGGFSAFNWTALQRKVLVPLVTSPAAGFFIALSLMIGLTWMLRR
                     SRAATVNRMSRRAQLVSSSLMAFAHGSNDAQKSMGIITLALLSFVASGDSHHLPAWML
                     PKQASGQWGVPLWVVIACAGAIAMGTAAGGQRIIKTMGSKIIRISPIQGCVAETAGGL
                     TILFASWLGIPVSTTHCISASIMGVGAAKRLSAVRWGVAGNIVTAWVLTVPISGTVAF
                     GMYRLLAYLFASS"
     gene            complement(13105..13764)
                     /locus_tag="PKL17_13745"
     CDS             complement(13105..13764)
                     /locus_tag="PKL17_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNK45846.1"
                     /translation="MPFQSVIRWLLPKDDRFFSLLEEHAGVLAEAAKQMLDLPATPNI
                     SGNIRQVQEILDRIHQLEHRGDDIVRAVMLALDETFVTPIDREDIHSLATALDNVLDY
                     LYKAAQSFVAFEMHELTQAMREMVQLLAECTTHIKDTLPFIRAHKLEKVAPARMQVVA
                     MEKRGDSIYQNEMASLFKNPQVDAKELLRQQTVIEALEAALNSCQDAVDVLENVAIKH
                     A"
     gene            14116..14904
                     /locus_tag="PKL17_13750"
     CDS             14116..14904
                     /locus_tag="PKL17_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45847.1"
                     /translation="MGGDVADLTTAPAATATTIRDLNSPNNTIKPGTKVKVSGVVTSP
                     LRWVDADTSAGYCYYRVHIVQTEPAPATLGDGMILTLSLKNSSFTDGSTLTQCKDRAK
                     TDTVAMAMDTLAMGQMVEVEGSYDTRANCGGTTRQLNMFGGKIVSQGMAATMPTPVDV
                     DPAKYPVATGTPKALAQTFVDGHAALVRFVNPKSYGRNATYQDFSVSTTGTAPGAQIT
                     TNYIRAQGMYTSLPDGTTYKSVTGIVLGDFCGTIWPRNQMDLVP"
BASE COUNT         2757 a         4798 c         4560 g         3010 t
ORIGIN      
        1 tttgtctacg tcgacaaggg cagcatggcc accatcggtc gcgccgctgc cattgcccac
       61 atcggtcgac tcaacatcgg cggcttcctc gcttggatgt tgtggctttt ggtgcacatc
      121 ttctttttga tcaccttccg caaccgcatc gccgtgctgt tcaactgggc ctaccagtac
      181 ttcacctata agcgcggcgc ccggctcatc accggacatc gcttgaaggc tggcgcagaa
      241 tcggacgttg ccgtcactgc cctgccaaaa agcgcgctta ccgccgcact acttccgcct
      301 gaagctgcga aaacgacgcc cgcagcacca ccggaaacgg ctccgcctgc ggtccctcgc
      361 taagccgaat cagcagttcc tgccccgtcg tccgcccgag aggccacagt gggacgtgat
      421 gtgcttgcgc ccgcagccgc gcgtccgctt ggcggtccgc ccaaatcccc agcaggtggt
      481 ggtgccccgc atcgccaagc agccgaattc ccgtcggatc gcccacgcac agagcggaag
      541 gcagcaccac cgtggccccc acgggcgtat cgcccaggca gcttgcgaca agtccgctga
      601 gcaggttggt ggaagacagc gccagatgac tcgccacgag cgactcggct tttagctcga
      661 tcagcagctc ggcaagtccg gcgtgtgcag tccccggttc cccaagcaag gccagaagca
      721 gacccggacg gccaaacgga accgcactgt gcgacgacag atcgagccga tgaagacagg
      781 acaaaagctc cacggcgggc atcgtaggct cgcttttgca atgaacccaa gtcagaaatg
      841 ggacattctt cctttgcata ccccatggca gaaccactgc tgtctgtaaa aagcctgcgc
      901 gtcgactacg aagacatcac cgcagtcgat gacgtatcgt tcgaggtcga ggccgggatg
      961 gtctatggac tgataggtcc aaacggcgcc ggcaagacct ccatcatccg tgtcattgcg
     1021 accctgcttc agccgacgta tggccaggtt cggctctgtg acattgacct tttgggcgac
     1081 cctttgcggg cgctttctca cctgggcttt atgcccgaca tgccaccgct gtacgacgac
     1141 ctctacgtcg aggagttttt gcagctgttt gcctcggcct atggtctttt cggcgctgca
     1201 caaacgaagc ggatcgatga actgctcgat aaagtgcagc tttccgaaaa gcggaaaacc
     1261 ccagccggag agctgtcccg cggcatgaag cagcggctgt ttctggccaa gaccctgctt
     1321 cacgacccca aagtgctgct gcttgacgaa ccagcatcgg gacttgatcc gcgggcgcgg
     1381 ctcgatctgc gcgatgtcat cgtcgagctt ggtcgcgccg gcaaagccgt ggtggtgagc
     1441 agccacatcc ttgctgaaat ggccgacttc tgtaacagcg tcggcatcat ggaacgcggc
     1501 aagctgcttt tgtccggtcg gatcgacgac atcttgaaac agcttcgccc cggtctgctc
     1561 attcgcgtcc gtctgctatc gcccgatgac cgaatcggac cgctgctcgg cggctcccca
     1621 ggggtcagtg aagtcgaaac cgacggcgcg cagtcgactt tcaagctggc cggggatggt
     1681 gaagcggcgg ccaacttgct gcgtgatctg attcagaaag acttccgcgt cgccgaatac
     1741 cgcgagatga cctcgaactt gcaggacatc ttcttgcagg tctcaagtgg gagggtgtca
     1801 tgagcgagcc cgtcgtccgc cttgtcagaa acccgatcct gattgcccaa gcgcggcgtc
     1861 ggctgcggcc tgcccagctt ttgtcatcga cgctgatctt cgggttgctc ggaatctgcg
     1921 gcgtgctgct gtccgtggtc gccaaagacc ggcacgaggc cttttcgagc tttgaatatg
     1981 tgctgctgac cggcattggc ggcctgctgc ttctgcgcgg cagcaatcgc gtcgccgaaa
     2041 ccgttcgcga ggaacgcgag agcggcatcc tcgactttca ccgcgccacc cccaccacac
     2101 cgtggaccga tggaatcgga tacctgctcg gctgcccggc gcgcgaatac ctgatggcgg
     2161 gcattctggc cggctttgcc ctcgcctgcg cgaccgcatc gcagtttgga cttctcccgg
     2221 tgctcggttc gctcgtcgcg attggcacat cgggactgct ctatcactgc tttgcgctgt
     2281 ggatcggact gtcggtcgct cacaagcgag gtgcgtcggg catcgtcatg ggcacgctgg
     2341 tcgtcctgct catcggtggc tggtcggccc gtggggtcgg tgccgtcagt tacttcactc
     2401 cgtacccggc gctcttttgg ctcctgtcga gcaaggaagc gaagataacc gaggtgacgt
     2461 tttacgggct gccgatgcat gcgctcgttt tgacgttgct tgtgcaaggt agcgtgcttt
     2521 tggcgatggg cacggcggtc gcgcgcaagc tgcgccaaga cgatgcgacg tcgttttcgc
     2581 gtctgggcac gctggccttt ttcgcgtgga tcgcctttct gtcgctcggc gccgcgtggc
     2641 agtggatatc gccggcttcg ggcgggccgc tttccgtcac cgcaagcacc gacactgcgg
     2701 tgatcgcggg ttatctcctt gtcagcacca tcgcagcggc agcgcttttg attggccagg
     2761 tgccgtccta tctgtcgctg cttcgtgcgc tgcgtcgcgc ccgcaggctc aagtccgcca
     2821 ccatcagctg gctcgacgaa ggcggtcgct gcgagccact gaccttgggc ctttggggtc
     2881 tgacggtggc cgggcttttg gccatccacc acgcgatggg tgtgacgcgc gtaagtgggc
     2941 cgctcctggg aaccccgctg cttttggccg ctgcggcagc gctctgctat ctgctgtttg
     3001 ttcaaaccgc gaccgagttt gtccgcatcc ggcatcgtgg ggccacgtca gccgtcgcgg
     3061 tgtttgtgtt cttcgcctcg catgtgctgc cgttcctgct gagctttgtg tttggtgcca
     3121 gcaaggccac accgggactc ctggtcatgg cggcaagccc catctatggc gtctgggcgg
     3181 cggcggctca cctgaccgaa gcctggctgc cgagcgtgca ggacaaaaca gagccactta
     3241 gcatctacgt gtctttggcg gtctcactcg cggtgtcggc ttttttcttt atcgaaagcc
     3301 gccgcagcca agccgacctg gcgcgaaaaa tcgccgattc gaccaagccc agctaaccca
     3361 cgatgaccgc gacttctgcc cctgggctgt ccgccaaatc gggccgcacc atcggttttg
     3421 ccttcgtcct tggcgccgcc ctcagctgga gcctggccgg catcggcatc aaatcggtgg
     3481 ctttgccggg cctggcgatt gtcggcctgc gcagcttgct ggccctgccg gtgtttgcgc
     3541 tggtgttgtg gctgcgggcg ggacaaaacc tggcgctggt gctgcggctc ggacttggac
     3601 gccccatcac ttggctggcg tccgcttgct atgcctggac catgctgtcg tttgtgctgg
     3661 cggcgaagct gaccaccgcg gccaatgcca tcctgctgca gtacaccgcg ccgatttatg
     3721 tcgtgctttt gtcctggccg ctgctaggcg aaaagattgg tgtcgccgac gtcctcgccg
     3781 tgcttggctg ctttttgggc attgcatggt tttttgtcga gcagctctcg acttccgggc
     3841 tgtacggcaa ccttttggct ttgtcatcgg gggcaggttt gggtcttctg ccgctgctct
     3901 tgcgccggac cgagcggcca gacccgagcc acgatgcgca aagccgcgag cttttgccct
     3961 ttgtccccac gctggtgctg cttcttggca acgtcctggc cgtgctcgct gggctggctg
     4021 cgatgttgcg tcagccggtt cccggcctgt cagcctggct gtggcttgcg gtccttggcg
     4081 ttgtgcagat cgcgctcgcc tatctgctgt atgccgccgc ggtgcgcaga ctgcgcccag
     4141 tggaatgtct gatgctggcg tcgctggaac cgatcctcaa tccgatctgg gtcgcgctgt
     4201 ttgccggcga gctccccagt cgcgccgcca tctgcggggg actgctcatc ctggcatcgg
     4261 tgaccggcca cggcatctac tcggcgctca ggccgcgaca ctagttccgc cgaacctgca
     4321 cttctgacat actcgcgcgc catgtcgctt gtgaacgacg agcccgcgcc gtcccccgag
     4381 atcctggccc ggctgcgccc cattcgcctg ctgtgtctgg atgtcgatgg ggtgctaagc
     4441 gatggccatt tgtattttgc cggtggcgaa ggcgacgccc gtttctgtca gcgcttttcg
     4501 gttcgggatg gcgtcggcat ccggctgctt atgcagtcgg gacgacacgt cgccatcctg
     4561 tccgaaggca acatcctgtc cggtcgggtt cgcgccgagt cgctttcgat ccgtcatgcg
     4621 tactttggcc tgcgcgacaa agtcgccaag ttttccgagc ttctttccga actgggcctg
     4681 tccaaaagcc aagcggcctt catcggtgac gaagtttcgg acctgccgct gctgcatgaa
     4741 gtcggctttg ccgccaccgt ccccgatgcc gtttgggaag ttcgccgcgc cgtccaccat
     4801 gtcaccaccc ggcccggtgg cagcggggct gttcgtgaag tctgtgacct gattcgccgc
     4861 ttcggtgata ccacaccggc aggaacaatc tgatgtcgag cgccgtgcta ctgactggag
     4921 ccactggatt tcttgggcga gacttgctgc ggaagttcgt cgaagagcta ccgcctgagc
     4981 agaccgtcta ctgcctgatt cgcggccacg cgcagggcaa agacaaagac cagcatcttt
     5041 ccccgcagca gcttgccgac aagcggctgc acaccctgct ttccgagctg ggctttcacg
     5101 atcgcagcga tccgcggctg cgtcgggtgt ctgccatcgc gggcgacatc agtcaggacg
     5161 ggcttggcct aagccaagct gcttatgcgg agctcgccgc ccaggtcgag cacatctatc
     5221 acggtgcggc gaccgtccgt tttgaccagc cgcttgccga agcacgccgc atcaacgtcg
     5281 aagggacccg gcagattctc gatctggcgc tgctggcaaa acgcagtggc cgcgcggtgc
     5341 gactcagcta catcggcact tcgtttgtcg ccggcacacg ccgaggccgc atctacgaag
     5401 acgagctggt ggtcgatcag gtgttccaca acacctacga gcagaccaag gcggaagccg
     5461 aagcgctggt acagaaagcc atcgccgagc acggtctacg cgccaccttg tttcgtccat
     5521 ccatcatcgt cggcgactcg cgtaccggtg aaacctccag cttcaaggtc atgtactggc
     5581 cactgaagat tttttcacgc gggctcatcc cgattgtgcc cgcttcgcgc gcgggcgtcg
     5641 tcgacatggt cccggttgat tatgtgattt ctgcgatttg ggagctgtcg cggcggaccg
     5701 atactgtcgg gggctgctac cacttggctg cggggcccga aggatcgacg acgattggtg
     5761 aagcgctcga ccaggccgcc gccttttttc gcgtccgcaa gccgctgttt gtacccaccg
     5821 agacattcga gcgctacatt cgcccgctat tccacctgtt cttccgtggc aagcgacgac
     5881 aagcgctcga cgccggacgg gtctacgtgc cctatctgaa ctaccaggcg tcgtttgaca
     5941 ccgaaaagac ccgcactgcc ctgcgcggga ctggcatcgc gccccccagc gtccgcgatt
     6001 actttgcgaa gcttatgcgc ttttgcgtcg acagcgactg gggcaagcgg accatccacc
     6061 cgtcagcgcc gcgccggcct ggtcagtagc tcatacaccg cgacgcagac cgactgttgg
     6121 ccatgtcgct ggcggctttt cacgttgtga acagcgtgca gtagctgggt cgcgcggtgg
     6181 ataagtcggg taagcggcac aaaatcgacc gctgccggtg gtcggccccc cggaggatcg
     6241 gcgacgcact gatcggcggt atcgagtgca tgggcattgc ggcagcccag cggcctgacc
     6301 gggtagatgc tacaggcccc atcgaccaga aaggcacagg ggatggcccg gcggaagtgt
     6361 tcaagatgca gcgcgtcata ttctgcttgc cggccttcga caaatgccgc agtcgcccgc
     6421 tcagggacat tgcccgcctg ctctttccat cgcggatacg cggccaaaaa agcggccttg
     6481 gcgtctgcat gttctggctg attcaggtag gcggcgattc gcagcgcttc gggcagatag
     6541 ctgacgacca acacggcaca gcaggcggta caacccttct ggcagtagat gtgaaagcct
     6601 tggcgcgcag cttgtgcagc acgggcagcc accccttcgt ccagctccgc ggccagtgct
     6661 tcggcaatcg acatcgcctc ggcgtcactt atcgccagcg caaaacgctt cgatccaccg
     6721 gcatagcggg gatcactcgc cagctcggtg cagatacgcg atagccgttc ggtgtccggc
     6781 atggatcact tgggcgctgg ggcttgggcc ggcggtgtct tcaccgcggc ttgtgtattg
     6841 gcgctttgca gctctttgag aagcggaccg gcgttataga ccccgcgcag cacctcgaac
     6901 aggcccgagg tgtggaccga gacggcccgg ttggctgtgt cgtcatagaa aaacgcaccg
     6961 ccaagcgatg ggatgttgcc gtcgaagatg gttccgacca cctgcgcatc gcggtttagc
     7021 accggactgc ccgagttgcc gccgatgatg tcgagcgtgc ttacaaagtt gagcggagtg
     7081 gacgccttga gacgcccttt ggccgccagc cacgagggcg gcagcacaaa cggttcccgc
     7141 ccggtgtgcc gatcgaacgc tccgccaagc agcgtaatcg gagacagctc ggtgctgcct
     7201 tctttgtacc cccgcaccgt gccgtaggtg acgcgcagcg aaaacgtggc atcggggtag
     7261 accgatgtgc cttggactgc aaacagcgcc tgggccacca gctctgagtt gcgcttttct
     7321 accgactcca cggagtcctg gtactgtcga cgaatcaagc gggactgcga atccactgcc
     7381 ttggcaaaac gaatcatcgg gtcggtcgat gctgcgatgg ccttctggcc tccgtcccac
     7441 agtgttttgc gcaccgccgg atcagccagt cgcgatccca tgatcaggtt gcgggcgatc
     7501 tcctcggggg aatacggtcc aaaggtctct ttgacaaacg ggtggtttgg cccaagcgcc
     7561 tcgcgcagcc gcccaagcga ataggtcagc gtcaggatct caagctgggg atacatcgtt
     7621 gatcgcgcca aaagctcatg cgaaagcgat ggcagtgccg agtcgcggta ctcgctgagc
     7681 cgctggtcgg agggcttggg ccgttcctcg gctgcccgca caagctgccg cgcatagtga
     7741 aacagcgtcg agttgaacgc ccgccccact tccagcagcg tgtgctcttt gtaaatcttg
     7801 cgcagctcgt cttgggctcg cgacagggca tcccaggcac cgccgtagcg tctttgccag
     7861 tcgggatttt gcgcgacctg gctgcgcagc gcttcttcgg atgaccgctt ggccgacagg
     7921 aagttccggc tggtcagggt ctcttgctgg ccaacaagcg ctttgtacca gttctcgacc
     7981 ccgtagagcg gctcgatcat gatgcgacgg ttttccggcc ccagcttggc aaactcgagc
     8041 atgtgccccc gcagctcggc caagtacagc atcagctcgg ggtttgcgac atcgcgcaga
     8101 aactctagct gcgacaccgt catgagtcgc tgggttgatc cggggtttcc agtcgaaaag
     8161 accagctccc caggggttac gccgacgggg tttaccggaa agaaattcgg cgtcttgagc
     8221 ggctggccgt tgtcgtagac gcgcacaaac gacacatcga gcgaatagcg cggatagttg
     8281 aagttgtccg gatcgccacc aaagcgcgcc gtcgaagtct caggcagaaa gaccagccgg
     8341 atatcggagt atcgacggta cttgtacagg tggtacttgc cgccgtgata cagcgtcaca
     8401 acgtcgcagc gcagcttggt cgccgacgcg cagtcttgtt ccagtcggct tatctcccgc
     8461 cgcaaaacca cgccaaactc ggcatctgaa agccctttgg tcgcggcttg caggcgactg
     8521 gtgacatcct caatgtccaa aagctgatgg aactccagcg atgaacactt gagctcactg
     8581 gcgtggctgg gcgcatagta gccattgcgg cttaggtcac gctgcgccga tgactgctcg
     8641 acaatgcaat cgccgacaca gtggtggttg ctcatcacca ggccgtccgc ggacacaaat
     8701 gcccccgagc agccattggc gagccgcagc gaggccagct gcactttctt gagccactca
     8761 tctgtgacct gaaagccata cttgcgactg atgcgctcac gcggaaaatc ggtgagcgtc
     8821 cacattccct cgtcggcccg aaccggcagc gcgaagtgcg agagcagtag cgccaccaaa
     8881 acacccagca cccgacccat gatcttgtgg gactggccgg cggacggcac tagcaaagcg
     8941 ggggacattc ggcctcctgg ggcgtttctt tgtcgagcgc gaaggctcgg ggccgatgat
     9001 tttccggtta agtagagcct gtcaagggtg cagcatgtgg agtggcgcgg ttgccggcca
     9061 ggccgggcgg ggaagctacg gggctgtcac aagctcgaaa tcagcggcac gcacccaggc
     9121 cacccgatgg ttgtagtgga tctggtagta ctcggtgccg tctttgacga cgatctgctt
     9181 gctcgggtcg taggccgaaa caaactcttt tgcccagtag tagtcacccg tggcgcgcgc
     9241 cgcgagcgca tagcgatggc ccgatggcac gtcgtagatt ttttccatca ctgccggctt
     9301 ggtgggcggc atgtacgccg cgtcttgcgg gtacgccacg ccatagaccg gtgccgtcga
     9361 tagaccgctc ttgggcgtga ccatcatcgt ggccgccttg cgggtcaagc tgcgtcctgg
     9421 gtttagaaac cacgccttct ggccaccaaa gtaaatcgcg tcccagtcgc ctttttgttc
     9481 tgcccgcacg tacatcggcc cggtccccag cttgtcgccc cagttgttca tgcggtcgcc
     9541 cggcacgccc tttaggtacg ggttctcaat cagcggcgcg gaaaggtccg gcgcggtgcg
     9601 cagatagaca aagttggccg gcgcttcggg cacatccttg cagtccgacg gcatcgagcc
     9661 atagcagtag ctaccttttt gcaggtttgt gtcgtagcca ggcctcagca caagggcgtc
     9721 cccgtctttg ccactgtcct tctcactcgg cagcggagcg agcaatagct gcatgtagcg
     9781 atcccagtcc cagtacggac cggggtccca gtgcatgccg ccctgcttgg ccgcagtgac
     9841 accggggatt tcgtcgtggc cgatgatgtg ttcccggtcg atgggaacgc cgtagctctt
     9901 ggcgaggtgg cgcaccagct tggccgagtt ttcgtacatc gcatcggtga accacttctt
     9961 gccctcaatg gccacggcct cgtgctcaat gccaatcgag tgcatgttga agtaccagtt
    10021 tcccgcgtgc catgcgacgt gacttggcgc gacaaacttg gccactcgcc cgtttgtgga
    10081 gcccaccaga tagtgggccg ccgccgatgc agtcgggttt tggaagtgcg agaccacacc
    10141 gtcccaagcg ccctcggtgg tgtggatgac gatgtaccgg atcggcatcc cgtccttggg
    10201 acgattggcc aggtcgtagt tgccgtaatc gccaggtgtc gcgctgcgcg ccttgtaggc
    10261 cgccgggatg tcctcgcact tgagctcgcg cggacagtcg gggtcctcac tggggggcgg
    10321 cggcggcggt ggcggcggcg gcggcatcac cgtatcgtcc cccgtcaccg gttccggcgg
    10381 ctcacagccg atcatcgtca gcgccaaaag ctgtccacag atcagcgaac ccacgaaaaa
    10441 cgaggagact ttcgagcgtt gaatcgtcac ttggcacctc ttgctggcgg tcaggtgccg
    10501 atttgtacca cgtcagcgcc ccctgcccag atcgggctta ccgacaaacg gattgggggc
    10561 ctgctcctct cccagcggat cggcttccgc agttttttgc tgcgtcaccg caatcgatct
    10621 gccccccctt cgactgagga ctggcgctcg cttgggctcg atccgatcga cctgatgctg
    10681 ggccggttgg gcagccagtg cagggctcgg cggcaacggc agtggtgcgc cggctggtgc
    10741 cggaccaagc ggatcggacg atggcggcac gggacgaggt gccacaaccg gcggtgccaa
    10801 gatcggacgc tgcacccacc agcgcgaacc aaacagacca accagcagca aacacagtgc
    10861 accaaaaaac atcgcctgcc gtagcgtgtt gctgagcgtc cctgggtcca tcgcagggct
    10921 cgttggcact gcatgcggca gcgccagcgg cggcaaacca tcccgaaaac gccgcaagtc
    10981 tgccaaaagc tctgccatcg tcccgtagcg caggtcccga tctcgcgcca tggcatgcat
    11041 cacaatcgac tctagacccg ctgaaatctg tcgatccgga caacgcgaag acggtggctc
    11101 ggcggactcg gtcaggatct tggccaccac cgataggtaa ctcgctgcgc agtgcggcac
    11161 gctgccggtc aagcactcgt aaagcaccac ccccagcccg tacacatcgg cccgtgggtc
    11221 aagtgcatcg ccacgcacct gctctggact catataaagc ggcgttccca ggatggcccc
    11281 ggtgcgggtc agtcgcggtt cgtcgtttat ccgcacagct ttggcgatgc caaagtcgag
    11341 cagtttcaca aagtccccat cgtcccgagc gcacagaaag atgttttctg gcttgatgtc
    11401 tcggtgcagc acgccgcttt catgcgctgc agatagagcc gtcgccagtt gctcgccaat
    11461 ccgcagcgta tcgacctctg atagcgccga acagcgggcg agtcgctggg ccaagctctc
    11521 gcccacaagc agctccattt ccacgtaggg acgaccatcc gcggtcaagc cgacatcgaa
    11581 gacctcgacg atgtgcggtg aagaaatcga cgatgccagc cgcgcctcgc tgtgcagtcg
    11641 cgaaaccagg tcttctgtca acgcaagctg ggcatttagc actttgaccg cgacctgctt
    11701 gtgcaggtgc aggtgctcgg cgacgtaaac ggtccccatc cctccctggc cgatccgccg
    11761 tcgcaccttg agcttgccgc cgagcacttc cccaaccagg gtgtcgtccg cgtctgcgct
    11821 gtccggaccg atgctagatc gtccgattgg agcctgcact gacaggtggg gcgtcacccc
    11881 atgaccgtag tccccagctg gcgcaaccgt cagcagggct ccaccatcga gcgggcaagc
    11941 atgcgtgccg tccgcgtagc gggtcaagca ctcagcgcac tgcaacataa cgatgggaag
    12001 ctagctgctc gcgaacaaat acgccagcaa gcggtacatg ccaaaggcca ccgttcccga
    12061 gatcggtacg gtcagcaccc atgcagtcac gatgttgccg gccacacccc agcgcaccgc
    12121 agacagtcgc ttggcggcgc ccacacccat gatcgaggca gagatgcagt gcgtcgtcga
    12181 caccgggatg ccaagccagc tcgcaaacag gatggtgagc ccaccagcgg tctcggccac
    12241 gcagccttga atcggcgaga tgcggatgat cttgctgccc atcgtcttga taatccgctg
    12301 accacccgcc gccgtgccca tcgcaatggc gccggcacaa gcgatcacga cccacagcgg
    12361 aacgccccac tgtccgcttg cctgcttggg cagcatccac gctggcaagt gatgcgaatc
    12421 gccgctggcc acaaacgaca gaagcgccag cgtgatgatg cccatcgact tttgtgcatc
    12481 gttagagcca tgcgcaaagg ccatcaggct cgatgagaca agctgcgctc gtctcgacat
    12541 tcggttgacc gtcgctgccc ggcttcgccg cagcatccaa gtcaggccga tcatcaggct
    12601 cagcgcgata aaaaagcccg ctgccggcga cgtgacgagc ggcaccagca ctttgcgctg
    12661 gagcgccgtc cagttaaacg ccgaaaagcc acccttggcc accacggctc ccgccagcga
    12721 accaatcagc gcatgcgacg agctagaggg aatcccgaac caccacgtaa acaggttcca
    12781 gaagcaggca ccggacaggg ccgccagcac caccacttgg ctgaccatct tggggtcggt
    12841 aaagcctgcg gcgatggtct tggcgactgc ggttccggtc agtgccccgg tgaagtcgag
    12901 gaccgccgcc atcagcacag cagtacggac cggcaacacg cccgttgaca ccaccgtcgc
    12961 gatggcattg gccgcgtcat gaaaaccatt gatgtagtcg aaaatgagag cgaaggcgat
    13021 gaccaggacg agcagggtca gcatgccagc atgctcctat gttcagcact gcagacagac
    13081 gctaccgcgt ttcgaccact gaatttaggc atgcttaatc gccacgtttt caagcacgtc
    13141 aactgcgtct tggcagctgt tgagcgctgc ttccagggct tctatcacgg tctgctgccg
    13201 cagcagctct ttggcatcga cctgcgggtt cttaaacagt gaggccatct cgttttggta
    13261 gatggagtct ccgcgttttt ccattgccac cacctgcata cgggcgggcg ccaccttttc
    13321 cagcttgtgg gcgcggataa acggcagggt gtccttgatg tgggttgtac attcggccaa
    13381 gagctggacc atctcacgca tggcctgggt cagctcgtgc atctcgaaag cgacgaacga
    13441 ctgggctgct ttgtacagat agtcgagcac gttatcgagc gccgtggcca aagagtgaat
    13501 gtcctcccgg tcgatgggcg taacgaacgt ctcatcaagg gccaacatca ccgcccggac
    13561 gatgtcatcc ccacggtgtt ctaactggtg gattcgatcg aggatttcct gaacctggcg
    13621 aatgttgcca ctgatgttcg gtgtggctgg aagatcgagc atctgcttgg cggcttctgc
    13681 caaaacgcca gcgtgttctt ccagcagaga aaagaatcgg tcgtctttcg ggagcagcca
    13741 gcgaattaca ctctgaaaag gcatgaaaga gtccttcgac tcgaagcgct gtagacagcc
    13801 tggttcttct gacccaataa ccgtcgacat catatcaggt cggtgggtct tttgggtaag
    13861 ccttttcgca actttgggcc ggctcggcga gacgcggctt ggacgggccg actttgtggc
    13921 ccctggtctt tgctgcctca gcgatttgcc agaggccagg tttgcgacta gggttcggcc
    13981 caagcaaaac caaaatcggc gctccaaccg cccaggaaaa gaggttttcc gatgaagcga
    14041 tcactccttt tgtgtgtcct cgccccgctt tggcttgtag cttgtcgccc ggctgacccc
    14101 agtgcacgga cggatatggg gggagacgtc gctgacctta cgacggctcc agccgcgacg
    14161 gccaccacca tccgcgacct aaacagcccg aacaacacga tcaagccggg caccaaggtc
    14221 aaagtcagcg gcgttgtaac cagtccgctg cgctgggtcg atgccgacac ctcggcaggc
    14281 tactgctatt accgggttca catcgtccag accgagccgg cccccgccac actcggggat
    14341 ggaatgatcc tgacgctcag cctgaagaac agcagcttca cggatggatc cacgctgacg
    14401 cagtgcaagg atcgggcaaa gaccgatacg gtggcgatgg cgatggacac cctggccatg
    14461 ggacagatgg tcgaggtcga aggcagctac gacacccgtg caaactgcgg cggaacgacc
    14521 cgacagctca acatgtttgg cggcaagatc gtaagtcaag ggatggcggc aacgatgccc
    14581 actccggttg atgtcgaccc ggccaagtac ccggtcgcca ctggaacccc caaggcgctg
    14641 gcccagacgt ttgtcgatgg acatgcagcg ctggtgcgct ttgtaaatcc caagagctac
    14701 ggacgcaacg cgacgtacca ggacttcagt gtcagcacga cgggcactgc acctggcgcg
    14761 cagatcacca ccaactacat ccgagcccaa ggaatgtata cgtcgctgcc cgatggcacc
    14821 acgtacaagt cggtgactgg catcgtgctt ggcgacttct gcggcacgat ctggccgcgg
    14881 aaccaaatgg atctggtccc gtagccggtt ccctcttcac aggaaagacc tcgtggcacc
    14941 gcggtcatag ccactgcgac gccatcgtct cgtctgtctt ggggaaagac cccgcctgat
    15001 cgactcatcg agtcgccagc cttggggaaa gaccccgcct gatcgactca tcgagtcgcc
    15061 agccttgggt aaagaccccg cctgatcgac tcatcgagtc gccagccttg ggtaaagacc
    15121 ccgcc
//
LOCUS       DAORGN010000124        14904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1648_length_14904_cov_7.091723, whole genome shotgun sequence.
ACCESSION   DAORGN010000124 DAORGN010000000
VERSION     DAORGN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_14904_cov_7.091723"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKL17_13755"
     CDS             complement(<1..1346)
                     /locus_tag="PKL17_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK45848.1"
                     /translation="MQFPSQLAKQTSKAAVALARATWPLVKVLNEKFERPSPHPSWAP
                     GPLLKTRERTKPQLGWPRETDSLCPKCVKEVRDDILSGRRKLTDLVDGRPGEVKARIV
                     ERDGQIIMEKSCEKHGVCVDVMSIDPKFMARIESLFPGRDFPTVETTLRSHGSSSMKY
                     GRGAVMTIDLTNRCNMMCDPCFMDANQVGFVHELSWDEIKQILDDTINIKPRRQMSIQ
                     FSGGEPTLSPHFLDAIRYARDVGYFAVQCATNGLRFAMEPGFAQKAKEAGLRMAYLQF
                     DGTTNESNAHRKIANLFDVKLRAIEELHRAGIDVILVVTLVNGVNNDQVGKIVNFAIE
                     NSDKITVVSFQPVSFTGRDEDVDDETRKKQRYTLSHLVHDMKDQLGVTEPMRDWFPLS
                     AMGPFSNLTDLLIEQNAKFGAVNCGCHPNCGVGTILFVNKRTKQMVPLLEFLDVEQ"
     gene            complement(1741..1959)
                     /locus_tag="PKL17_13760"
     CDS             complement(1741..1959)
                     /locus_tag="PKL17_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45849.1"
                     /translation="MPEPLRFVSIGTDHGDSGAAAGAVALLHAVASFSPPTWAQTAPV
                     ESALVYGVDDGGAMGACLVASPQSRFAA"
     gene            complement(2136..2498)
                     /locus_tag="PKL17_13765"
     CDS             complement(2136..2498)
                     /locus_tag="PKL17_13765"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNK45850.1"
                     /translation="MAKRAPLWPADVDPHFLSAAAPGLFASPHLQGNENVVLTGLHPL
                     GTIQFRLPHWRLAAKFRFAHHTERRRLLLDAVFLAPDQLTVTLIWRTSVPARPSMLNL
                     RQCILRVLEPWEQSPAHE"
     gene            complement(2704..3216)
                     /locus_tag="PKL17_13770"
     CDS             complement(2704..3216)
                     /locus_tag="PKL17_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45851.1"
                     /translation="MTLPQNYVAFLRFMGSAHGGFRVFPEHDYLIQDLLNRPHEGEDW
                     DRRRYFLIGFQDPRQSREDPHSLFLDLKRSDGIDAPIVGFQPGPSPTVMSIEHGIADF
                     LIFRIAWLFAMDDKPARARLISFCDSSPDGHPARDRHREIKQTVERLGFAPCLPATPY
                     SWFGQRDDET"
     gene            complement(3876..5429)
                     /locus_tag="PKL17_13775"
     CDS             complement(3876..5429)
                     /locus_tag="PKL17_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNK45852.1"
                     /translation="MKLVAVLRPLGLWAALAVGSVGGTSAAGANGGPSTTPPAGVVRL
                     VIDGEPVHLNPLLDPDLWGHRIVHDLICEPLIRRSESESGPVYEPVLAERFRLDKDGR
                     GLDLWLRKGVRFHDGRPLSAYDVQLSLQMVLASEHVAPQTRALLRDVVRVVKNGNESI
                     HLDLRRGQTQILDALSQVSIVPAAHFPDGRLIQKPWSRKPICTGPYRFVEWKRGSQIT
                     LRRFPGYHGPPAAAEELRFLIAPDSARGLHLLRNGQAEVMLRVPLRYLPDLINPAVER
                     GRWRKLEPLANQLVTLVYNGRHPLLGQAAARRALASLLDQKRLLRDARFGQGSQDVIL
                     PSQPRQTVEQAGSLLDALSATRAAPGSARLWQGRPISLRLLCPQGSSELLDLATHIAQ
                     ALAPLGIKVESEAVELSALTARLRRGGFDLALLGWGWTGSDRLFDPEPLLHFAYPDSH
                     PLWTQLGGRLLDLQLGGDLKNLGEVWQREEPLTLLYRPRQLLIAQPGISLTQQADFLN
                     LRTLHLSSP"
     gene            complement(5426..7258)
                     /locus_tag="PKL17_13780"
     CDS             complement(5426..7258)
                     /locus_tag="PKL17_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNK45853.1"
                     /translation="MLTAFPLLFGLSLPAAPVAALASSDLRTVAEQSGFARTGRYDEV
                     ITLCERFEKTFPQKVRCQQFGQTPQGRPMLALVASADGTFSPEDIAQKSRPVVLAQGC
                     IHAGEVDGKDAGFWLLRSLLSGETLPGVLGKLTFVFVPVFNVDGHERFAAHQRPNQSG
                     PVETGWRVGAHNLNLNRDYAKAETPEMLAMLGLLRRYDPLLYIDLHVTDGADFQPDVA
                     VQIEPRLGGPAPLRAIGSDLSQRVLDELRRQGHMPLDFYPTFVKKDDPSSGFVQEVPP
                     PRFSTGYWPRKNRFAVLIETHSWKPFERRVRATYHAVSALLRNVAERGKDWRMTAQQV
                     DHSDEKRPPGSRIPLGYETGPSKVELDFPGYAYKIEPSPVSGALRISYDPQKPQVWKI
                     PFFPTVVEKTSIPLPAGYVVPPEHAGWLSDKLRAHGLQFSTVQDSHNGPCAVYRVKSR
                     TFADAPYEGRHTVSVEGEWKTESCSAAKGSLWIPTAQRGATLVAQLFEPDSSDSLLRW
                     GFFNSIFEQKEYLEDYVAEQVAEHLLASDATLRKEFQQRLTNPAFAQSPTARLQFFAE
                     RHPSYDKSHNRYPILRFDSPLPTRRPVATTPERYGQNTQVDSAR"
     gene            complement(7302..7718)
                     /locus_tag="PKL17_13785"
     CDS             complement(7302..7718)
                     /locus_tag="PKL17_13785"
                     /inference="COORDINATES: protein motif:HMM:NF039673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HNK45854.1"
                     /translation="MKIQNTFVLCAEDCPVDHAVVPQEKSGKPTVASLEHQLLSQTPY
                     HYTRDDLLVEVALRKQGLSAPEREAQREALRTELLRRPQACMRASPLPKSYGFGVHYD
                     GEGRLAIYEKGSPDYARLAAASDVTVVKAMRNRRSS"
     gene            7855..9420
                     /locus_tag="PKL17_13790"
     CDS             7855..9420
                     /locus_tag="PKL17_13790"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNK45855.1"
                     /translation="MSKTMRERVTELEAERERIKQMGGPDKVQKQHERGKLTARERLE
                     RFFDGGQYFEVGMFGTQMGGSNPTDKPPADAVICGFGPVNGRMVAAAAYDFTVKGGSI
                     GMTGETKVTRMRELALRARMPMVWFIDSAGARIDPQSQGGDQISLFAGSGHLFREEVL
                     MSGVVPLVAAMVGPGAAGTAYIPGLADFVPMVKNQGSMALGGPPLVKAVTGQEITEQE
                     LGGSKIHCETSGVGDVEVPDEESCLRLIKEYLSFMPQHCDEKPPIIPCDDPLERRDDA
                     LLDVLPQSTRQSYDMYQIVKTIVDHGRILDIKPKWAKNIITCLARIGGYPVGIVANNP
                     RHIGGILDVNSADKASHFMQICDAFNIPLVFLMDVPGFMVGSKVEHEGIIRHGAKMLH
                     IMASATVPKLTVVVRKGYGAGYYVMAGRAYEPDLLVAWPGAEISVMGAEGMVGIAGKK
                     LFGGMEPPAEVKAQIVGMIQQHIDILKVAGWGLCDDVIDPRDTRRMLGLGLKMAWNRT
                     VERPARKRGIIPV"
     gene            9534..10997
                     /locus_tag="PKL17_13795"
     CDS             9534..10997
                     /locus_tag="PKL17_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45856.1"
                     /translation="MSKTRLSVWLAMGAVLCGCGDSGAGGTTIGPAGPVGPAGPEGKS
                     GNQAPALTLFTPQVAFAGRTVSVQISGGGTAFTGSEVIDFADVAINTDSVSAGSVSNL
                     RASVSIGPSAQVGPHDVTVVSGTDVFKLAGGFTVAASLKPNTLTAPSVGQGGLIPYDV
                     LNVDYQVSPFSSGFRLNGGLHTVSQTSLSATRTVGMGLADALIPVGAFGYQASFVDPV
                     ARPITYTTDPADSLSPKATARTATAVTLTVALPGQNFAAPLSDNLYQLSTSANDQVLF
                     GQFIGLGSAFAGGNVTVGAAYAGSDGKFGGGQLVPTSANLAANTRTMLAYAPQMGTGY
                     IASWPTDFGGGAVAQYGHSFLLSALTGTKLALTEPAIPDTAAAPLATIASLAATSPNF
                     ATDGQADFAGDSDFIRFTAAAGTTRLYVQVVAPGASVQLVEHSSGSCAAQVSSTTATH
                     ALAYENTATPAAQRCLQVVLPSAPKYPWPYRLIVYAR"
     gene            11087..11689
                     /locus_tag="PKL17_13800"
     CDS             11087..11689
                     /locus_tag="PKL17_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNK45857.1"
                     /translation="MKTGTLVSSLRISSLLVASLALFACGQAETEQNEAEGFPAPIAT
                     DVVDSDRTAQAVDPTQLAKFRADMLVAVNKARSVARTCGTTSYPAVPALKANSKLDTS
                     SQGHAASMAAKNFFSHTGLDGSQPWDRMKRAGYNYSAAGENIAAGNSTVDATMKQWLS
                     SPGHCANIMSRNFTEIGIGYDYGASSTYKHYWVQNFGKPL"
     gene            complement(11720..14170)
                     /locus_tag="PKL17_13805"
     CDS             complement(11720..14170)
                     /locus_tag="PKL17_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45858.1"
                     /translation="MKTGSTHQTLFRLCTPILRHFRRSFATLLLFLLFLSGCANGERE
                     FPIEGAVTTYTGSWEYRYGPCPADSLQRPQCLNAEWNDGSFVSGGVESPSGRDGRNEL
                     WLRTRLTGPRLSDPVMYLKFVDQFYEAYVDGKLIAKSPGSLFSTARLTGVYRAYLPLG
                     SDYVGKTLVVRVHSRYWHIGIHREQLIGERATMIASAVRNGLSAIMIGSMLMVLALIG
                     LTLAVVQRQRAFAMYGSFALLSGIYLLARSQLRIFLFGSQELARLFELSALALLAAAL
                     CGFVEALFGPGPLRLLRILKRTFVAFFIGSVALISIGIVPVEWLLFPLQILLLAMVLV
                     MLVNALRIGSLGHIDGRILSVGLAIALMPTLYDLLSAMNVIRRYVVLTQYAVALFVLT
                     IGLIVVRRFMESRGEKVRLAMEAAQVKARLAEQDALLQAFRRVADGDLDSPIEVAADS
                     SLKSLADALNRLREDVRSRLKQLSAKNLAAKQLNDELRRQIEQRSRRLLNLMIQRPLK
                     TKEQSQLKVQSLLGEHYRIVCMLGTGASGVVLEVERITDGKRLAAKVLTNAQDQTGVV
                     RFLREAQILSRLDHPNLVSIQDVDITEEGVPFLVMELVEGQTLRQLRNRYRDRRFALQ
                     ALKQIADALKAIHDGGIIHRDLRPSNVLVVDDGDTPRIKLFDFGVAALRAKEGTESGT
                     YPKQKPGVSVSSSADRIELSLPGLLIGSSMYMAPECRGGPQAVGSAADVYSLGVIAWE
                     LLTGELPFSRPPVEMAAQQEPLTPPSLHTKIPDVNPALWALISSCLHPDPGKRISAAQ
                     VAAELALQVEEQQAQTAG"
     gene            complement(14174..>14904)
                     /locus_tag="PKL17_13810"
     CDS             complement(14174..>14904)
                     /locus_tag="PKL17_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45859.1"
                     /translation="GIAKLADSSKGAFRTGTNMLLGTPIYMSPEQCRGAKNVGDRSDV
                     YALGVMLFEMLAGRPPFETEMPGEYIALHIFQPPPSLRQFVPNVDERLEQLLLSMLAK
                     DPKIRPPMLEVAQRLKFLGNLQSDQLGRGPHERSSGPLPEMQPAKLPGPKPDDATEIL
                     PHNMGPPDWQVMNVVAGRQQTPTSVVEALKPQVTRPSGERKRENLRPSGFLTPQRRAQ
                     LVALLTISIAICIGILITLLLVGS"
BASE COUNT         3027 a         4227 c         4714 g         2936 t
ORIGIN      
        1 aactgctcga catcaaggaa ctcgaggagc ggcaccatct gcttggtccg tttgttgaca
       61 aacaggatcg tgccgacgcc gcagttcggg tggcagccgc agttgacggc accaaacttc
      121 gcgttttgct cgatcagcag atcggtcagg ttggaaaacg gacccatggc cgacagcggg
      181 aaccagtcgc gcatcggctc ggtgacgccg agctgatcct tcatgtcgtg gacgaggtgc
      241 gacagcgtat agcgctgctt tttgcgcgtc tcatcatcga catcctcatc gcgaccggta
      301 aagctcaccg gctggaacga tacgacggtg atcttgtcgc tgttttcgat cgcaaagttt
      361 acgatcttgc cgacctgatc gttgtttacg ccattcacca gcgtcaccac caggatgacg
      421 tcgatgccag cgcggtggag ttcttcgatg gctcgcagct tgacgtcaaa caggttggcg
      481 atcttgcggt gggcgttcga ctcgttggtg gtgccgtcga actggagata cgccatgcgc
      541 agaccggcct ctttggcttt ctgcgcaaaa ccgggctcca tcgcgaagcg caggccgttg
      601 gtcgcgcact gcaccgcgaa gtaaccgacg tcccgtgcgt agcgaatcgc atcgaggaag
      661 tgcggcgaca gcgtcggttc accaccagaa aactggatgc tcatctggcg gcgcggcttg
      721 atgttgatgg tgtcgtcgag gatctgcttg atctcgtccc aggacagctc gtggacaaag
      781 ccgacttggt tggcgtccat gaagcacgga tcgcacatca tgttgcagcg gttggtcaga
      841 tcgatggtca tgaccgcgcc acggccatat ttcatcgacg agctaccgtg tgaacgcagc
      901 gtggtctcga ccgtcgggaa gtcccgtcca gggaacaggc tctcgatccg cgccatgaac
      961 ttgggatcga tggacatgac gtccacgcac acgccgtgct tttcgcagct cttttccatg
     1021 atgatctggc cgtcgcgctc gacgatgcgg gccttgacct caccggggcg accatccacc
     1081 agatcggtca gcttgcggcg tcccgacaga atgtcgtcac gaacttcctt gacgcacttt
     1141 ggacatagcg aatccgtctc acgcggccag ccgagctgcg gcttggttcg ttcgcgggtc
     1201 ttgagcagcg gaccaggcgc ccacgacggg tgtggcgagg gacgctcgaa cttttcattg
     1261 aggactttga cgaggggcca agtggccctc gcgagggcga cggcggcctt cgaggtctgc
     1321 ttggcaagct gggaagggaa ctgcattggg aaactcctaa gaaactcagt actcgctgcg
     1381 tttaaacgcg ccgcaatcct agcagcaagc aggtgcggct cgttcctcat agatacgcaa
     1441 aacaacgatt agagtgaacc aatagttcag tgtttcagtg atcttaccga ttccgacggg
     1501 gggtggggtc ttgaccccca cccaaagggg gtgaaaatcc cccccagttt ggcccgcttt
     1561 gggagtccga taaggagggc tacgcgcagc catgggggtc ttccgcgaga ccactcccaa
     1621 tcgacaaaac cacaagcgca cacgcaaacg gaatcgcacg ttcggaatcg cagcctaatc
     1681 cgggatggaa agccacccca gcgcagaaat caggtcgggg agggagtccg tcgtctgctc
     1741 ttacgcggcg aaacggctct gcggtgaggc gacaagacat gcacccatcg cgccaccatc
     1801 atcaacgcca tacacaagcg cgctttcgac gggagccgtc tgggcccacg ttggcggcga
     1861 aaagctcgcc accgcgtgaa gcagcgcgac tgcaccggct gctgcaccgc tgtctccgtg
     1921 atcggttccg atggaaacaa aacgcagtgg ctccggcatc catgcggtcg cttcggctgg
     1981 ccgatggggg aatcgtcgag agtctgctga ccgtggcgtc gaatgcgtcc gtctgttcaa
     2041 accgcgtgat gccagcgcgc atggccgcaa gcgacgcccg gctacaaaga cccagcggac
     2101 aagtgagcgc aaactgggaa atggcaagga gaggactact catgtgcagg gctctgctcc
     2161 cacggttcga ggacgcggag gatgcactgc cgtaggttta gcatgctggg acgcgcaggc
     2221 acgctggttc gccagatcag cgtcaccgta agctggtcag gtgcaaggaa gactgcgtcg
     2281 agcaggagcc tgcggcgttc ggtgtgatgg gcgaagcgga atttggctgc gagccgccag
     2341 tgcggaaggc gaaactggat ggtgcccagc gggtgcagtc cggtcagcac gacgttttca
     2401 tttccttgca gatgcggtga tgcgaaaagc cctggtgccg cggcggacag gaagtgggga
     2461 tctacatcgg cgggccacaa gggggcgcgt ttggccacct atccgttcca gtccgcaaac
     2521 agccagcttg tccttcccaa cgcaggctgg actgacccgg atgtccaaca tagctgtcgg
     2581 caagtggcac gggcggctgt tccgaaagag gcacgagtgg ttcatcgaaa gcactggttc
     2641 cgggcgcggg cattgcaaag cacccagcga ccacaaggag cacgatgtcc tcgttggcca
     2701 ggttcatgtt tcgtcgtccc gctggccaaa ccacgaatac ggtgtggcag gcaagcacgg
     2761 tgcaaaccca agccgttcca ccgtttgttt gatttcgcga tggcggtctc gtgcgggatg
     2821 gccgtccgga gacgagtcac aaaaagaaat cagccgcgcg cgggccggct tgtcatccat
     2881 ggcaaaaagc caggcgattc ggaagatcaa gaagtcggcg atgccgtgtt cgatgctcat
     2941 cacggtgggc gatggtccgg gctgaaagcc gacgatgggc gcatcgatgc catcgctccg
     3001 cttgagatcg agaaacagcg aatggggatc ttctcggctc tgacgtggat cttggaatcc
     3061 gataaggaag tagcgccgac gatcccagtc ctcgccttcg tggggacggt tcagcaggtc
     3121 ttggatgaga taatcgtgct cggggaaaac acgaaatccg ccgtgcgcgc ttcccatgaa
     3181 gcgaagaaac gccacatagt tttgtggaag cgtcatcggt gcactatgtt gcaattcatc
     3241 tagctctgtt cgtgatgatc caatgatttc acgagccgtg tgtggctgtc gcttttcgat
     3301 gaatgccaga aattcagatc cggtgatcat gggcggccct ctaacacgat cgatctttgt
     3361 cgcacaacat cattggtagc aagatttggc aagtgtggca cgatgcgacc cctttcggct
     3421 catcaagaat tcgtgacgcc tggttatgaa agggaatctt cgatgtggtc ccggcgatag
     3481 tgacgcgcaa atcgtaccat ttctaccagc ggtccatttt cgatggactg tacatcatct
     3541 cgctcatcgc caaaagactt atcaccgaat ctaggaccac gacattggcc cccgacgatc
     3601 gagcgattgt gtcaaaatcg gatggggtat ctcccgatgt cccgacaaga attttgttac
     3661 atttgcaaga tgcgaccgca accatgaaac catctgggta atccaatttt tgcagctttt
     3721 tgatgatttc ggcgcaaatc gcggcggatt ggtactgggc cgcctggggt tggtgtcttg
     3781 cagacatccg gcggtgccga cgtgttcgtc ggaccgtcgc ctttgtgcac gatggaacga
     3841 ccgtttgcga ataccgtggc cacgctagat cctcgctatg ggctgctcag gtggagggtg
     3901 cggaggttga ggaagtcggc ttgctgggtg aggctgatgc cgggctgggc gatgaggagc
     3961 tggcggggac ggtaaagcag ggtcaggggt tcttcgcgct gccagacttc gccgaggttt
     4021 ttgagatcgc cgccaagctg aagatcgagc aggcgaccgc caagctgggt ccacagcgga
     4081 tggctatcgg gataagcaaa gtggaggagc ggctcgggat cgaacaagcg gtcactgccg
     4141 gtccagcccc agccgagcag cgccagatca aagccgccac ggcgcaggcg ggcggtgagc
     4201 gcggacagct cgacggcttc ggattcgacc ttgatcccaa gcggcgccag cgcttgcgcg
     4261 atgtgtgtcg ccagatcgag cagctcactt gaaccctgcg gacagagcag gcgcagcgaa
     4321 atcgggcggc cttgccacag ccgggcgctt ccgggggcgg cgcgagtggc cgaaagcgca
     4381 tcgagcagac tgccggcttg ttcgacggtt tgtcggggct ggctcggcaa aatgacgtct
     4441 tgtgaaccct ggccaaagcg ggcgtcgcga agcagtcgtt tctgatccaa aagcgacgcc
     4501 agcgcccgac gcgccgcggc ttgtccgagc agcgggtgtc gcccgttata gaccagcgtc
     4561 acgagctgat tggcgagcgg ttcgagcttg cgccagcgac cgcgctcgac ggctgggttt
     4621 atcaggtcgg gcagatagcg aagcgggacg cgcagcatga cttcggcttg gccgttgcgc
     4681 aaaagatgca gtccgcgggc actgtcggga gcgatgagga agcgaagctc ctcggcagcg
     4741 gcaggcggtc catggtagcc gggaaatcgc cgcagggtga tttggctgcc gcgcttccac
     4801 tcgacgaagc gataggggcc ggtgcagatc ggcttgcggc tccacggttt ttggatcaag
     4861 cgaccatcgg gaaagtgggc ggcggggacg atcgaaactt ggctcagcgc gtcgaggatc
     4921 tgggtctgtc cacgccgcag gtcgagatgg atcgactcat tgccgttttt gacgacgcgg
     4981 acgacatcgc gcagaagggc gcgggtctgg ggggcaacgt gttccgatgc cagcaccatc
     5041 tggagcgaga gctggacatc gtaggcagac agcgggcgac cgtcgtggaa ccggactcct
     5101 ttgcgcagcc acagatccag gccgcgcccg tctttgtcga gccgaaagcg ctctgcgagg
     5161 accggttcat agacgggacc actttcgctt tcgctgcgac ggatgagcgg ctcgcagatg
     5221 aggtcgtgga caatccgatg gccccacaga tcggggtcga gcagcgggtt taggtgaact
     5281 ggctccccgt cgatgaccag gcgcaccaca ccagcgggcg gggtggtcga tgggccaccg
     5341 ttagccccgg cggcggatgt ccccccgacg ctgccgacgg ccagcgcggc ccaaagcccc
     5401 agcgggcgga gcactgcaac cagcttcatc gcgcggaatc cacctgggtg ttctgaccat
     5461 atcgctcggg cgtggtggcg acggggcgac gggtcggcag cgggctgtca aagcgcagaa
     5521 tcgggtagcg gttgtggctc ttgtcgtagc tcgggtgccg ctcagcgaag aactggagtc
     5581 gggcggtggg gctttgggca aacgccggat tggtgagccg ctgctggaac tctttgcgca
     5641 gcgtggcgtc gctggcgagc aagtgctcgg cgacctgctc ggcgacgtag tcttcgaggt
     5701 attccttctg ttcgaagatg ctattaaaaa agccccagcg caaaagcgag tccgaggagt
     5761 ccggttcgaa gagctgggcg acgagcgttg cgccgcgctg ggcggtcgga atccacagcg
     5821 agcctttggc cgcgctgcac gattcggtct tccactcgcc ttccaccgag acagtgtggc
     5881 ggccttcgta gggggcgtcg gcaaacgtgc gggactttac ccggtagacc gcgcacggac
     5941 cattgtggct gtcttgcacc gtcgaaaact gaagcccgtg ggcgcggagc ttgtcggaaa
     6001 gccacccagc gtgttcgggg ggcacgacgt agccagcggg cagcgggatc gaagtttttt
     6061 cgacgacggt ggggaaaaac ggaatcttcc agacctgcgg tttttgtggg tcgtagctga
     6121 tgcgcagcgc cccggatacc ggcgatggct cgatcttgta ggcgtaaccg ggaaagtcga
     6181 gttcgacttt gcttgggccg gtttcataac ccagtgggat gcggctgccg ggcgggcgtt
     6241 tttcgtcgct gtgatcgact tgctgggcgg tcatgcgcca gtctttgccg cgctcggcga
     6301 catttcgcag caaggccgaa acggcgtgat aggtggcgcg gacgcggcgc tcgaacggtt
     6361 tccacgagtg ggtttcgatg agcacggcga agcgattttt gcgcggccag tatccggtcg
     6421 aaaaacgcgg cggcggtact tcttggacaa accccgagga cggatcgtct tttttgacaa
     6481 acgttgggta aaagtcgagc ggcatgtgac cctggcggcg cagctcatcg agaacccgct
     6541 gcgacaaatc actgccaatt gcgcgcaggg gggccgggcc gccgagccgg ggttcaatct
     6601 gcaccgcgac atcgggctga aagtcagcgc cgtcggtgac gtgcaggtcg atgtacagca
     6661 gcggatcgta gcgccgcaaa agaccgagca tcgccagcat ttccggggtt tcggctttgg
     6721 cgtagtcgcg gtttaagttc aagttgtggg ctccgacgcg ccagccggtt tcgacgggtc
     6781 cgctctggtt gggtcgctgg tgggcggcga aacgttcgtg gccatcgacg ttgaaaaccg
     6841 ggacgaaaac aaacgtcagc ttgccgagca cgccgggcag tgtttcgccg gacagcaggc
     6901 tccgcagcag ccaaaaaccg gcgtctttgc catcgacttc cccggcgtga atgcagcctt
     6961 gggccaggac cacggggcgc gacttctgcg cgatgtcttc gggcgaaaac gtgccatcgg
     7021 cggacgccac aagcgccaac atgggccggc cctgcggcgt ttggccgaac tgctggcagc
     7081 gcaccttttg ggggaatgtc ttttcaaagc gctcgcacag cgtgatgact tcgtcgtaac
     7141 gcccggtgcg agcaaaacca ctctgctcgg cgaccgtgcg caggtcggaa gacgcaagcg
     7201 cagccacagg agccgccggt agcgagagac caaagagcag cggaaaagca gtcagcatgg
     7261 cgaagactta gcagaaaacg ggcagggaaa aacgggtggg attagctgct gcggcggttt
     7321 cgcatggctt tgacgaccgt tacatcgctg gcggcggcaa gtcgcgcata gtcgggactg
     7381 cctttttcat agattgcgag ccgtccttcc ccatcgtagt gcacgccaaa tccatagctt
     7441 ttcggcagtg gcgaggcgcg catgcacgcc tgtggacggc ggagtagttc tgttcgcagc
     7501 gcttcacgct gggcctctct ttccggggca gaaagtcctt gcttgcgcag cgccacttcc
     7561 accagcagat cgtcgcgcgt gtagtggtac ggcgtctggc ttaggagctg atgttcaagg
     7621 gacgccaccg tcggcttgcc ggacttttcc tgcggcacga cagcgtgatc gaccggacag
     7681 tcttcggcgc acagcacaaa ggtgttttgg attttcattg cctgcatcct cggtcgtttc
     7741 tggctttgga ccgtgcgctg cacatagcac tttttggcgg atgcttctca agctggctgg
     7801 gtttgtgcga gtgttgtgga ccacggtgtg cgcgaaactt cggagacaga agcgatgagc
     7861 aagacgatgc gagagcgggt gacagagctt gaagcagagc gtgagcgcat aaaacaaatg
     7921 ggcgggcccg acaaagtgca gaaacagcac gagcgcggca agctcaccgc acgcgagcgt
     7981 ctggagcgtt ttttcgacgg cggacagtat tttgaagtcg ggatgtttgg cacccaaatg
     8041 ggcggttcaa atcccaccga caagccgcca gccgacgctg tgatctgtgg ctttggcccg
     8101 gtcaacgggc gcatggtggc cgcagccgcg tatgacttca cggtcaaggg cggatcgatt
     8161 ggcatgacgg gcgaaaccaa agtgactcgc atgcgcgagc tggcgctgcg ggcgcggatg
     8221 ccgatggtgt ggtttattga ctcggcgggg gcacggatcg atccgcagtc gcagggcggc
     8281 gatcagattt ccctgtttgc tggatcggga cacctgtttc gcgaagaagt gctgatgtcg
     8341 ggcgtggttc ctcttgtcgc tgcgatggtc ggtccggggg cggcaggaac cgcgtacatt
     8401 ccgggtctgg ccgactttgt tccgatggtg aaaaaccaag gctcgatggc gcttggcggt
     8461 cctccgctgg tcaaggcggt gacaggacag gagatcaccg aacaggaact tggtggcagc
     8521 aagatccact gtgagacatc cggggtcggc gatgtcgaag ttcccgacga agaaagctgt
     8581 ctgcggctga tcaaggaata cctgtccttt atgccgcagc actgcgatga aaaaccgccg
     8641 atcattccgt gtgatgatcc actagagcgg cgggatgatg ccctgctcga tgtgctgccg
     8701 cagagcacgc gccagtcgta tgacatgtac cagatcgtaa aaacgatcgt cgatcacggg
     8761 cgcatcctcg acatcaagcc aaagtgggcc aagaacatca tcacttgtct tgcgcgcatc
     8821 ggcggttatc cggtcggaat cgttgccaac aatccgcgtc acattggggg catcctcgac
     8881 gtgaacagcg cagacaaggc gtcgcacttt atgcaaattt gcgacgcttt taacattccg
     8941 ctcgtgttct tgatggacgt tcctggcttc atggtcggta gcaaagtcga gcacgaagga
     9001 atcattcgcc acggcgcaaa aatgctgcac atcatggcat ccgcgacggt tcctaagctg
     9061 acggtggtgg tgcgcaaagg ctacggtgcg ggctactatg tgatggcagg acgggcgtat
     9121 gaaccggatc ttttggttgc gtggccgggg gccgaaatca gcgtgatggg tgcggaagga
     9181 atggtgggaa tcgccggcaa gaagctgttt ggcggcatgg aaccaccagc ggaagtgaaa
     9241 gcgcagatcg tcgggatgat ccagcagcac atcgacatct tgaaagtcgc gggctggggt
     9301 ctgtgcgatg acgtgatcga tccgcgggat acccggcgga tgcttggttt gggactgaag
     9361 atggcgtgga accgcaccgt cgagcgcccc gcccgcaaac gcggcatcat tccggtctaa
     9421 gcccgcagca aaaagagagc gcgattcctt ccggctcggc gtattacact gaaagcgatt
     9481 tcgtcttcgc gagatcgctt gttcaggatg ccagcttcgg ggggaaacag cttatgtcaa
     9541 agaccaggct ttcggtgtgg cttgcgatgg gcgctgtgct ctgtggttgt ggggacagtg
     9601 gtgcgggcgg aacgacgatt ggtccggcgg gtccagtggg gcccgcaggt ccagaaggaa
     9661 agtccggaaa ccaagcgccg gcgctcacgc ttttcacgcc gcaagtggcg tttgcgggac
     9721 ggactgtcag cgtccagata agcggcggtg gaacggcgtt tacaggcagt gaagtgatcg
     9781 actttgccga cgtcgcgata aacaccgatt ccgtttctgc tgggagcgta agcaacctgc
     9841 gtgcttccgt atcgatcggt ccgtcggcgc aagtcggtcc gcatgatgtg acggtggtat
     9901 cgggaaccga tgtgttcaag cttgctggcg gattcaccgt ggcggcatcg ctgaaaccca
     9961 atacgctgac ggcaccgtca gtggggcagg gaggactgat tccgtatgac gtgctgaatg
    10021 tggactacca agtgagtccg ttttccagcg gattccgctt aaacggaggt cttcatacgg
    10081 tgtcacagac ttcgctttcc gcgacacgca ccgtcggaat gggtctggcg gatgcgctga
    10141 ttccggtggg cgcatttggt taccaggcaa gctttgtgga tcctgtggct cggcccatca
    10201 cgtacacaac cgatccggcg gattccctgt cccccaaagc gacggcacgc acggccacag
    10261 cggtcacgtt gacagtagca cttccgggac aaaactttgc cgcaccgctg tctgacaacc
    10321 tgtatcagct cagcacatcg gccaatgatc aggtgctgtt tggtcagttt atcggactgg
    10381 gatctgcgtt tgccggcggc aatgtcaccg tgggcgcagc atatgcaggc agcgacggaa
    10441 aatttggtgg gggacagctt gttccgacgt ctgccaacct ggctgcaaac acccgcacga
    10501 tgctggccta tgcaccgcag atgggaaccg gatacatcgc gtcttggccg acagactttg
    10561 gcggaggcgc tgtggcgcag tatggtcatt cctttctgtt gtctgcactt acggggacaa
    10621 agcttgcgct gacagaaccc gcgattccgg ataccgctgc ggctccactt gcaacgatcg
    10681 cgagcctggc tgcaacgtcg ccaaactttg caaccgatgg gcaagcagac tttgcggggg
    10741 acagtgactt cattcgcttt accgcggcag cggggactac gcgcctgtac gttcaggttg
    10801 tcgcgcccgg cgcttcggtc cagcttgtcg aacactcaag cgggagctgc gccgcgcagg
    10861 tgagcagcac cacggcaacg catgcactcg cctatgaaaa cacagcgacc ccggcggccc
    10921 agcgctgtct gcaagtcgtc ctgccgagcg cccccaagta tccgtggccg tatcgtttga
    10981 tcgtctacgc acgctaactg ccgaccactc cttttccctt cccatgcgga agcttttgcg
    11041 gtatgcggcg gtttgattcc caaacccaag ccggaaggag ctcctcatga agactggaac
    11101 acttgtctct tcgctgcgta tttcgtcgct gctggtcgct tcgcttgcgc tttttgcctg
    11161 tggacaagcg gaaacggaac agaacgaagc agagggcttc cccgccccga ttgcgaccga
    11221 cgtggtggac agtgatcgaa ctgcgcaagc cgtcgatccg acgcagcttg ccaagttccg
    11281 cgccgacatg cttgtggccg tgaacaaagc gcgttcggtg gcgcgaacct gtggcacaac
    11341 aagctatccg gcggtgcctg cgctcaaggc aaacagcaag ctggatacgt cgtcgcaggg
    11401 acacgcggcg agcatggcgg cgaaaaactt ttttagccac acgggccttg atggttcgca
    11461 gccgtgggat cggatgaagc gggctggcta caactattcg gcagcggggg aaaacatcgc
    11521 agccggaaac tccacggtgg atgcgacgat gaaacagtgg ctgagcagcc caggacactg
    11581 cgccaacatc atgagccgga actttaccga gatcggaatc ggctacgact acggcgcaag
    11641 ctcgacgtac aagcactact gggtgcagaa cttcggcaag ccgctctagt cgtcattccc
    11701 ataccaggga atgtgattcc taaccggcag tctgggcctg ctgttcttcg acttgcaggg
    11761 ccagctctgc tgcgacctgg gcggcgctga tgcgcttgcc tgggtcggga tgcagacagc
    11821 ttgaaatcag cgcccacagc gcgggattca catcaggaat cttggtgtga agcgacggtg
    11881 gcgtaagcgg ttcctgctgg gccgccattt caaccggagg ccgtgaaaac ggcagctctc
    11941 cggtgagcag ctcccacgca atcacaccca agctgtagac atcggcagcg cttcccactg
    12001 cttgcggacc gcctctgcac tctggcgcca tatacatcga ggaaccgatc aaaagacctg
    12061 gcaaagacag ctcgatgcga tcggcagagc ttgaaacaga gacccctggt ttctgcttgg
    12121 gataggttcc tgactcggtt ccttctttgg cgcggagtgc ggcgacacca aagtcaaaca
    12181 gcttgatgcg cggcgtatct ccgtcgtcga caaccaggac gttactgggc cgcagatcgc
    12241 gatgaatgat tccgccatcg tgaatcgctt tgagtgcatc ggcgatctgc ttgagcgctt
    12301 gcagcgcaaa ccggcgatcg cgataccgat tccggagctg gcgcaacgtc tgtccttcga
    12361 ccagttccat caccaggaag ggcactcctt cttcggtgat gtcgacgtct tgaatcgata
    12421 ccaagttggg atgatccagc cgcgacagaa tctgtgcttc gcgcaaaaat cgcaccaccc
    12481 cagtctgatc ttgcgcattg gtaagcacct tggcggcgag tcgttttccg tcggtaattc
    12541 gctcgacttc cagaacgacg ccacttgcac cggttcccag catacaaacg atccggtagt
    12601 gttcaccaag caacgactgc actttgagct ggctctgttc tttggtcttg agggggcgct
    12661 gaatcatcaa attcaggagc cgccgcgagc gctgttcaat ctgtctgcga agctcatcat
    12721 tgagctgttt ggctgcgaga ttttttgccg atagctgctt gagccgcgag cggacatctt
    12781 cgcgcagacg attgagcgcg tcggccagag acttgagcga gctgtccgca gccacttcaa
    12841 tcggagagtc gagatcgcca tccgccaccc gccggaatgc ctgcaggagc gcatcctgtt
    12901 cggcaagtcg cgctttgacc tgcgccgctt ccatcgcgag cctgactttt tcaccgcgcg
    12961 attccatgaa tcgtcgcacc acgataagac caatcgtcag cacgaacagc gcgacggcat
    13021 actgcgtcag gacgacgtag cgccgaatca cgttcatcgc ggaaagcaga tcgtacagcg
    13081 tcggcatgag cgcgatcgcc aggccgacgc ttaggatgcg tccgtcaatg tgacccagtg
    13141 atccgatccg caacgcattg accagcatca ccagaaccat ggccagaagc agaatctgca
    13201 gcggaaacag cagccactcg accggaacga ttccgatgga aatgagggcg acgctgccga
    13261 tgaaaaaggc gacgaaggtg cgcttcagaa tgcgaaggag tcggagcgga ccagggccaa
    13321 acagcgcttc gacaaaacca cacaacgccg ccgccaaaag tgccagcgca gacagctcga
    13381 agagccgtgc aagttcctgg ctaccaaaca gaaagattcg tagctgcgag cgtgccagca
    13441 gatagattcc ggacaacagc gcaaaacttc cgtacatcgc aaaggcccgc tgccgttgca
    13501 ccaccgcgag cgtaaggccg atcagcgcga gcaccatcag catggatcca atcatgatcg
    13561 ccgacagacc gttgcggacg gcactggcga tcatcgtcgc tcgctcgcca atcagctgct
    13621 cgcgatggat tccgatgtgc cagtagcgcg agtggacccg aaccacgagc gtctttccta
    13681 cgtagtctga gcccagcggc agataggccc ggtagactcc tgtgagccgt gcggtagaaa
    13741 acagactgcc tggtgatttt gcgatcagtt ttccgtcgac gtaggcttcg taaaattgat
    13801 cgacaaactt cagatacatc accggatcgg aaagccgcgg tccggtcagt cgggtgcgca
    13861 gccacagctc gtttctgcca tcccgaccgg aaggactctc gactcctccc gacacaaatg
    13921 atccgtcatt ccattcggca ttcaaacact gtggtcgttg tagggaatcg gcaggacagg
    13981 gtccgtagcg gtattcccat gatccggtgt aggtcgtgac tgcaccttcg attgggaact
    14041 ctcgttcgcc atttgcacac ccggacagaa acagcaaaaa gagcagcagg gtggcaaaag
    14101 atcgacggaa atgccgcaga atcggcgtgc agagtcgaaa cagcgtttgg tgagtagaac
    14161 cggtcttcat gttctagctc cccaccagaa gcagggtgat cagaatcccg atgcagatgg
    14221 cgatgctgat ggttaggagg gcgacaagct gcgcgcgccg ctggggtgtt aggaatccgc
    14281 ttgggcgaag gttttctcgc ttccgttccc ccgatggtcg tgtcacctgc ggcttcagcg
    14341 cttcgaccac gctcgtcggt gtttgctgac gacctgccac aacattcatc acctgccaat
    14401 ccggtggtcc catgttgtgc ggtaggattt cggttgcgtc gtccggtttg ggtccgggca
    14461 gcttggccgg ctgcatttcc ggcagtggtc ccgaagatcg ctcgtgcggt ccgcgaccca
    14521 gctgatcgct ctgcaagttg cccaggaact tcaggcgctg cgccacctcg agcatcgggg
    14581 gcctgatctt gggatctttg gccagcatcg acagcagcag ttgttccagt cgctcatcga
    14641 cgttcgggac aaactgacgc agcgacggtg gcggctggaa aatatgcagc gcgatgtatt
    14701 cgcccggcat ctctgtttca aacggaggcc gaccagccag catctcgaac agcatcacac
    14761 cgagcgcata cacatcgctg cgatcgccga cgttttttgc accgcgacac tgctccggcg
    14821 acatataaat cggcgttccc agcagcatgt tcgtcccagt ccgaaacgcc cccttgctgc
    14881 tgtccgcgag cttggcgatg ccaa
//
LOCUS       DAORGN010000125        14823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1663_length_14823_cov_5.645856, whole genome shotgun sequence.
ACCESSION   DAORGN010000125 DAORGN010000000
VERSION     DAORGN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1663_length_14823_cov_5.645856"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKL17_13815"
     CDS             <1..408
                     /locus_tag="PKL17_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNK45860.1"
                     /translation="ATPGLQMVPGQVILAPDDAHLVVEYKGYVGLNHTGPVLGHRPSV
                     DKLLLSLAKSYGSAAVGVILSGIGSDGTAGLSAIREAGGLTFAQTGESSAVNGMPRSA
                     SESGAAMFCQHPSAIADSLRFSFAKEKVQDGSR"
     gene            395..1741
                     /locus_tag="PKL17_13820"
     CDS             395..1741
                     /locus_tag="PKL17_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNK45861.1"
                     /translation="MAADDAVKNLGLRIARWAGIDVSQRLGEVSRWLTMMCHSTDAAD
                     IAAMADQILSEDHPLLPDLVRSLTVSHTRFFRDIEQLDAVETLLRRGPSQMRVWVAGC
                     STGEEAYSVALLALAQHRKIEIVASDVNQASLDAARAGVYPPEAIERIPRRFLFDFTN
                     RGSYIEVSPRMRQAVRIVHHNLMKPPLRPTDGSGWDIILCRNVLIHFRAEHAAQALDK
                     LALALAPGGHLFLGTSEFHQRTPRLVPVHVANRIALQRPTRMPIAVPTQLTESPTPPP
                     IPTSGQPTTERKTPRTIEAPLSDQQLSSLLLAGSPAEVLRLTRAQLHEKPDRQPALLL
                     AGIAHHLLAHHAEAVQLLERAKAQHPTCWPVLHFLALSLDELGRKADAKHVYFQLQRH
                     PAPTPEAEALIDLLEFGPWYEEACALARHRTRGSLTGFRFTPQHTKHSDGDKVRSR"
     gene            1741..2136
                     /locus_tag="PKL17_13825"
     CDS             1741..2136
                     /locus_tag="PKL17_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK45862.1"
                     /translation="MAIRPIKVVVVDDSEFVLEITKHELEAQGLEVFTVNSPIGFSQL
                     LRDRKPDIALVDVSMPALQGPQLISIAHRHGATNICPVVLFSDRSRSELEQLASMCGA
                     AGVIRKSDDWEGIALEIRRILGRHHRPGH"
     gene            2214..3722
                     /locus_tag="PKL17_13830"
     CDS             2214..3722
                     /locus_tag="PKL17_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45863.1"
                     /translation="MATAPPHPPASPKAPGDGWFSSLWPRRHELLIAGRYLFRRAPSR
                     GLVGVSLALLAATAVVESLYFLVPGWQAPQMAIVALLLPVVTVVSGLLNILSVFSTVA
                     VLGVMLGVAALTVVMAVTSGFQAEIRSRVVGLNAHVLVLKYGIDFHEYEETLKKCQTH
                     PSVVAGSPFVYNEMLIAKEGALTSGVLVKGIDAARANAVLDLTRWLVPLPSGQKPLLA
                     SLDTDAAPSPGGPPLPGLFLGQELARKLKAQPGDKLRLISPQIGLDTLQGADPSQAAA
                     PRSQEFRLAGVFSSGFDEYDRRLVLTGLRPAQQLVGQGDVVTGLELRTQSVEQARQTG
                     GEVISLLGGAPYRSLDWEELNHNLFTALAMQKAVLSLVLFLIILVAAFNIVASLTMMV
                     IDKTREVAILKAMGMSSASVSSLFRVAGMAIGSLGVSLGLSLGVLTCKLIERLGYLLD
                     AHVYMIERLPTKVSGIELVITGSVTILISLLATVYPSVRAARLHPVDGLRYD"
     gene            3833..>4695
                     /locus_tag="PKL17_13835"
     CDS             3833..>4695
                     /locus_tag="PKL17_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45864.1"
                     /translation="MKRRLLSLGIPLSLALGLHAAEAQSVGTSTKTDGKDSGLSAPVK
                     PVAPVQETAKPAAAPAKWAKPAAKPAVKAAAKAGKVGIKTGAKAAAKPAAAPAAKAAA
                     DVAQAPAADAKADLVKAPAAEAKTETAAKDSAAAKPDAKPAQATKAAPAAKTEPKAKA
                     EAAAKPEPAAKPDPAAKPDPAAKPEPAAKPGTTAKPEPAAKAAPAAKTEPAAKTEPAA
                     KTEPAAKAEPAAKAAPAAKLEPAAKADPAAKADPAAQAEPATQPKSAAKADTAAKEIP
                     AAKGAAGKWKAV"
     assembly_gap    4696..4795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4796..8094
                     /locus_tag="PKL17_13840"
     CDS             <4796..8094
                     /locus_tag="PKL17_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45865.1"
                     /translation="AAKEIPAAKGAAGKWKAVKGKPVAPTANAKTHAAEKAAPEKLAP
                     EKAVAEKPAPEKLAPEKAVAEKPVAAPPKTAEAPAAKPAVAQPIAQPAPVPMPAPAPA
                     PAPVEARPSGPELPAFVNLEQGRRALLAGRNYEAALFLGKAYEQGVGEPLALEWLGDA
                     MRRVSSLESALVEQQADLKLARFFPSGQRILTLSWFGSLSLWDGTTGRRIPQPSVPSQ
                     PIVDAVLSPDGTRFYSIDNRSQVQIWDAERGAVLASLPAIDVSVTRLSSDGRRLLTVS
                     RGASPTDLIQLWDMATGRMLAELRGADAEGTTDVRFAPGARYVLIERTEAVHKIRVWD
                     TVENRMKLVEEGQRVQFSSDGRRAAVFGSGTRPQLFDLTTGKNYAQLRYQQTAIVEAQ
                     FSADGRRAITIHKSNDSDGEGAAQVACIWNVTEGSFVAVMAEKKGSIRQVVQSPDGKL
                     VVTMTDTGTASLWDIDSGRKLAVLDDDDENEEGKTKLAFGQSGRHVLIRNPSSLGVFE
                     LPSGRQVQLSGTIGTLQDASFSPSGLYLATVSTDGTVRFWVTATGQQVGVINQGGNPI
                     TDAQWSPESAYFGTIDKKHQLTVWDVAESKQRFSMPTPPSTTNLSFGSDVRYVSFVDT
                     QQRISIRRVEPGATAKTTRSLSEVATGHTVLPLTMQKAVDSQFSSDGQRLLVRDAGQT
                     FLVWDLRLGKQLVRLQGHADSPDGRRFSPDGRSLILMHPDGKTRLHDALTGKALAEVP
                     QGSLSFSPSSPRVAIGDARGRVTVWDLTHGRMVSQSTGLGAELRSVRWLPDGKRIAVV
                     SDLPIARVFDANTGRTVAEQRRLPPNAAEVAVSPDAKYAAVAIPATAKIPGTIKLFPF
                     DGSAPIQVQQGKTSTPPVVFDFSRDGKQLFIEAQGKSRWLRPIPAGDALVEQLGGVFA
                     WNDDSHRIFSAQPEMAPVVIDTQTGKKTWELPSAMQDFQKVQFGPGGTHLLSVHNDDS
                     VRIWETATGRLVMSLAKPQSPLRDAVLSPDGKRLFTTHDDDAIRQWHVETGKLVAELK
                     GLAGARILISPDSRTLFSTDDSGRHIVWDIRAEQRTPQEVAKWLRCYVPFRLDGTKLV
                     SAAANPSECK"
     gene            8178..8672
                     /locus_tag="PKL17_13845"
     CDS             8178..8672
                     /locus_tag="PKL17_13845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK45866.1"
                     /translation="MLSFSVTPFHELSPAALYELLALRQLVFSVEQNCAYLDCDGKDQ
                     KALHLLGSSDDGRLVAYARLLPPGVCYPEASIGRVVNHPAVRRSGCGRILMQEAISQL
                     VRAFGPVPIRIGAQAYLRRFYESFGFVTVSDPYDEDGILHILMLREKDQAPLQTEPHD
                     LARR"
     gene            8697..9722
                     /locus_tag="PKL17_13850"
     CDS             8697..9722
                     /locus_tag="PKL17_13850"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNK45867.1"
                     /translation="MQISHQTPDFARSADSYSFGVYRRAIANPNVCASGCLAPLRLKE
                     WHYVSVSTPRHFFACAVVRLGYAAKMFAYLVDREHPDRTVEASTLHPLSLGVRFASSS
                     IAGRTVWHKGNRSLQIEYVQSPTPGWNLDVNLELGPVVLQGQLHIEDAEALALLYKLP
                     TGNPAYTHKAAGLFLRGKLTANGQPIDLTDAMAGIDWTRSVAPRQTRWLWSALQGVTP
                     QRQRVGLNLSALVYDDENGNSQENALWVDGKVYPLGGVKFVLPKDPRKQPWRIHSRSG
                     GTKEVDLLFTPLGTREEHVHLGLIKSDFIQPYGTYQGTVCPTDSGIPSLSVDGLFGVV
                     ETHESVW"
     gene            complement(9821..10927)
                     /locus_tag="PKL17_13855"
     CDS             complement(9821..10927)
                     /locus_tag="PKL17_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNK45868.1"
                     /translation="MKLCPLIAAQVRPLSTQLHLPFHISQGSVELLENVLIVLRDADG
                     RVGLGEAAPFPVLTHDTQQTVLPACRAIVDQLSGLTPAAALDRLVRDLWPEFSQTPTA
                     LAGVEIALWDLHARQLGLPLAALWGRAFWSQAETDITLPRLSPSEVPVFYQQFADHHF
                     PYYKVKVGGGSVADDVERVMALRNIVGASLRISLDGNQGLSEASALSLCEALVRHQVV
                     PLFFEQPLPAADLDGMARLARRLPIPVCADESVQTIDDAFDVLHRSAAGMVNLKFMKS
                     GVSQALQIAELCHRKKIPLMIGGMVESEIAMTAALHAVCGTGIISVCDLDTPFFLTSR
                     FTKHSPYHGKSAMLQLPEGDGLGISLCDELFADS"
     gene            complement(10965..12062)
                     /locus_tag="PKL17_13860"
     CDS             complement(10965..12062)
                     /locus_tag="PKL17_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45869.1"
                     /translation="MYPVLVDGSAAERRRCRIPNAGANPTCVKMSGGIAALVRGHKAD
                     EVPGLIAPRLRQAWEMLGDPLRKGNPQLRDRIRGVVALGTGGFRNPSTNLPDDRQEFK
                     AVWTEIERFFHAEAKQEHVVAKTISGLMEARLAWLGVAQQVPASPKDSFAIIEVGGAS
                     IQFATSGRSLDYEDLYAASEYRGQDYVFNKFVTSSGQVTPGFDACYHPEDPTQQDGGS
                     CMAFLRKTVFWGSSINQLAFHFSSRRLYGLGAPWLGIFADWPSGPPWTLKADPSYNAN
                     LSTINLAALAKMVCKLHDEEIAAFAPNAFDLRKNPATGQSSGKACFTLSYHAEFLASV
                     IGIAHDGVLLPGGDDQWARGAAVTHEFFPDC"
     gene            12494..13057
                     /locus_tag="PKL17_13865"
     CDS             12494..13057
                     /locus_tag="PKL17_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45870.1"
                     /translation="MPTHEEQLRREQELFEKKEEAQKHSQLPQHAQVHEQSERLLAEE
                     QRVEQSRLLSSLKAEAFSPSQLAELPKQSEPTKAAVEQLFHHLVEKKDKPPTDAIKKT
                     QRTMTQVDRKTGLRSPEPKHREPDDLFATGIEGDFVPNTKISAADFDVHPSPDDGIDW
                     GGSGPRRRKRGQPSKAGIKKKPSGSDK"
     gene            13097..>14823
                     /locus_tag="PKL17_13870"
     CDS             13097..>14823
                     /locus_tag="PKL17_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK45871.1"
                     /translation="MSAQTESERQAAEQWLAAELNRMWLVAERCLRQFQRAQLRPQPD
                     NPSLAEVEGIFTARRAARGGLSSGPMDEAELSRAITDVESSLYALRRSAPIGRVIENL
                     GLSPLEVETLIVAMSPHIDAPLSDVFSLLRSSPSRRGVDLSLVSQLYQLRRSDRLQLL
                     DALDPERPLLAWRMIQIISPESMESYSSVNYRAIMPTFDVLSTLCGRGELSPALAKTS
                     KLLRAEKRLDDVVLDEQLRQKMERLCTMADHSVLQQQPQHMPWVMFYGAKGSGKKELA
                     SRLAAYCGRSLLWFDPTQAEQGTLDDLLRRAQREALLRGAVLYIGPLRGDLLNDNGRE
                     LIKRIISYPSMLVLGVEQTQPPRMTIDHSMQEVQLSIPPEPSRLILWQRVVPESIRAK
                     DLSLESIARAFHLSPGEIINTGEEAQELASLSPSGTVTHDQLRAGIDRRLRNDLGDMA
                     KRIAVSARWDDLVLPDDDILRIKEFIGRSKYAYRVYSDWGFGMRVGYGKGMIALFSGP
                     PGTGKTMLAGLIAQALDLDVYQVDLAQVVSKWVGETEKQLAKVFDAAERAHAVLLFDE
                     ADSLFAKRT"
BASE COUNT         3108 a         4554 c         4386 g         2675 t
ORIGIN      
        1 gcgacaccgg gtctgcagat ggtgcccggt caggtgattt tggcccccga tgacgcccat
       61 cttgtcgtcg aatacaaagg ctatgttgga ctgaatcata cagggccagt cctcggtcat
      121 cggccatcgg tcgacaagct gctgctgtcc ctggcaaaaa gttacggttc tgctgcggtg
      181 ggcgtgatcc tgagcggcat tggcagcgat ggaacggcag gactttcagc cattcgcgaa
      241 gcgggtggac tgacgtttgc gcaaaccgga gaaagctcgg cggtaaacgg aatgcctcga
      301 tctgcgtcgg aaagtggagc ggcgatgttc tgccaacacc caagcgccat cgccgattcg
      361 ctgcgcttct cgtttgccaa ggaaaaggtg caagatggca gccgatgatg cagtcaaaaa
      421 cctcggcttg cgcatcgcca ggtgggcagg tatcgacgta agtcagcgtc ttggcgaagt
      481 cagccgctgg cttacgatga tgtgccattc gacagacgcc gctgacatcg cagcaatggc
      541 cgaccaaatt ctgagcgaag atcacccgct gctgcccgac ttggttcgct cgctaacggt
      601 gtcgcatacc cgatttttcc gcgacatcga acagctcgac gcggtggaaa cactgctgcg
      661 tcgcggaccg tcccagatgc gggtctgggt ggccggttgt tcgacgggag aagaagccta
      721 ctcggtggca ctcctggcgc tcgcgcagca tcggaaaatc gaaattgttg catcggatgt
      781 gaaccaggcg tcgctcgatg cagcacgggc tggcgtctat ccccccgaag cgattgagcg
      841 gattccgcgc cgcttcctgt ttgacttcac aaaccgcggt tcgtacatcg aagtctcgcc
      901 gcggatgcgg caagccgtgc gcattgtgca tcacaacctg atgaagccac cgctacggcc
      961 cacggacggt tcggggtggg acatcatttt gtgtcgcaac gtgctcattc actttcgcgc
     1021 cgagcatgcc gcccaagcgc tcgacaagct ggcactcgcg ctggcccccg gcggacacct
     1081 gttcctggga accagcgaat ttcaccaacg cacgccgcgg cttgtgccgg tccacgtcgc
     1141 caaccggatt gctctccagc gtccgacgcg gatgccgatt gcagtaccga cgcaacttac
     1201 cgaatcgccg acaccgccgc cgattccgac aagcgggcag ccgacgaccg agcgcaagac
     1261 cccacgcacc atcgaagccc cgctgtccga ccagcagctt tcgtcgctct tgctcgcggg
     1321 ttcaccagcg gaagtgctgc gactgacccg cgcccagctc cacgaaaagc ccgaccgcca
     1381 gccggcgctt ttgctcgcag ggatcgccca tcacttgctg gctcatcacg ccgaagcagt
     1441 ccagcttctg gaacgcgcca aagcgcaaca tccgacctgc tggccggtgc tgcattttct
     1501 tgcgctcagc ctcgatgaac tggggcgaaa agccgacgcc aaacatgtgt attttcagct
     1561 ccagcgccac ccagccccga cgccagaggc agaagcgctg atcgatttgc tggaatttgg
     1621 tccctggtac gaagaagcct gtgcgctcgc gcggcatcgg acccgaggat cgctgacagg
     1681 ttttcgattt accccgcagc acaccaagca cagcgacggg gacaaagtga ggagtaggtg
     1741 atggccatcc gtccgatcaa agttgtcgtc gtcgatgaca gcgagttcgt gctcgaaatc
     1801 accaagcacg agctggaagc ccaaggcctg gaggtgttca cagtgaactc gccgatcggc
     1861 tttagccagc tgttgcgaga tcgcaaaccc gacatcgcgc ttgtcgacgt gtcgatgccc
     1921 gcgctgcaag gcccccagct catttcgatc gcgcatcggc acggcgccac gaacatctgt
     1981 ccggtggtgc tgttttccga ccgcagcaga agcgaactcg aacagcttgc cagcatgtgt
     2041 ggcgccgccg gagtcattcg caagtccgac gactgggaag gcattgcgct ggaaattcgc
     2101 cgcattttgg gccgacacca ccgcccaggc cactagatcc ggtgtggctg gtttcgtccg
     2161 ttttcgtccg ttttcgtcga aaaaggctgc tataagtcgt tttcttcgat ttcatggcaa
     2221 ctgcaccgcc acatcctccc gcatccccca aagcgcccgg cgatggctgg ttttcatcgc
     2281 tgtggccccg tcgacacgaa ctgctgatcg cgggtcgcta cctgttccgt cgcgcgccga
     2341 gccgagggct ggttggcgtt tcattggcgc ttctcgccgc aactgccgtc gtcgaaagcc
     2401 tgtacttttt ggtcccaggc tggcaggccc cgcagatggc cattgtggcg ctgcttctcc
     2461 ccgtggtgac ggtggtaagc ggcctgctca acatcttgtc ggtgtttagt accgtcgctg
     2521 tgctcggtgt catgctgggc gtcgcggcac tgacggtggt gatggcggtg acttcgggct
     2581 ttcaggccga aattcgcagc cgcgtcgtcg gtctgaacgc gcatgtgctg gtgctcaaat
     2641 acggcatcga ctttcacgaa tacgaagaaa cgctgaaaaa atgtcagacc catccgtcgg
     2701 tggtggccgg ctctccgttt gtttataacg aaatgctgat cgccaaagag ggagcgctga
     2761 catcgggcgt gctggtcaaa ggcattgatg cggcgcgggc caatgccgtg ctcgacttga
     2821 cgcgctggct tgtgccgctg ccgtcgggac aaaagccgct tttggcttcg ctcgataccg
     2881 acgcggcgcc gtcgccaggt ggaccgccgc tgccgggact ttttttggga caggagcttg
     2941 cgcgcaagct caaagcgcag cccggcgaca agctacgact gatttcgccg cagattggac
     3001 tcgatacgct gcaaggagcc gatcccagcc aagccgcggc gccgcgctca caggaatttc
     3061 ggctcgccgg cgtgttttcg tcgggctttg atgaatacga tcgccgcttg gtgctgaccg
     3121 gcctgcgtcc cgcgcagcag ctcgtcggcc aaggcgatgt cgtcaccgga ctggaactgc
     3181 gcacccaatc ggtcgaacag gcacggcaga ccggcggcga agtgatcagt ctgctcggtg
     3241 gtgcgccgta ccgcagcctc gactgggaag agctaaatca caacctgttt acggcgctgg
     3301 cgatgcaaaa agcggtgctg tcgctggtgc tgttcctgat catcttggtt gcagcgttta
     3361 acatcgtcgc cagcctgacg atgatggtga tagacaagac ccgcgaggtc gcgatcctca
     3421 aagcaatggg gatgagcagc gcatccgtat cgtcgctgtt ccgcgtcgca gggatggcca
     3481 tcggatcgct gggggtgtcg cttggacttt cgctgggtgt attgacctgc aagctgatcg
     3541 aacggcttgg ctatctgctc gacgcccacg tctacatgat tgagcggttg ccaacgaaag
     3601 tttcggggat agagcttgtt atcaccggat cggtcacgat cctgatttcg ctgctggcga
     3661 ccgtgtatcc atcggtgcga gcagcacgcc tgcaccccgt cgacggcctg cgttacgatt
     3721 aagcggcccg ccccgcgcct ggcacgcaat tccagatggc tttcaggccc atagccatcg
     3781 taaaatgagc cgctacccgc acaagcgggg cttgcccttg ggggggcggt acatgaaacg
     3841 gcggcttctt tcgctcggca ttcctctttc gctggctctc ggccttcatg cagcagaggc
     3901 acagtcggtc ggaacatcga ccaaaaccga tggcaaggac agcgggctca gcgctcctgt
     3961 caagcctgta gccccagtcc aagagacggc caagcccgca gcggcccccg ccaagtgggc
     4021 caagccagcc gcaaagcccg cggtcaaagc agcagccaaa gccggtaaag tcggcatcaa
     4081 aaccggcgcg aaagcagcag ccaaaccagc cgcagcgccc gcagccaaag cggcagccga
     4141 tgtggcccaa gcccccgccg ccgatgccaa agccgatctg gtcaaagcac ccgctgcgga
     4201 ggccaaaacg gaaacagcgg ccaaagacag cgccgcggca aaaccggacg ccaaaccagc
     4261 gcaggcgacc aaggcagccc ccgcggccaa gacagagccc aaagccaaag cagaggccgc
     4321 tgcaaagccc gaacccgctg caaaaccgga ccccgctgca aagccggacc ccgctgcaaa
     4381 accggaaccc gctgcaaagc ccggcaccac cgcgaaaccg gaacccgctg caaaagcagc
     4441 tcctgctgcg aagacagaac ccgctgcgaa gacagaaccc gctgcgaaga cagaacccgc
     4501 tgcgaaggcg gaacctgctg caaaagcagc gcctgctgca aaactggaac ccgctgccaa
     4561 agcagatccc gccgccaaag cagatcccgc ggcacaggcg gagcccgcga cccaacccaa
     4621 atccgcagcc aaagcggaca ccgcggccaa agagatccct gcggccaaag gtgccgccgg
     4681 caagtggaaa gccgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcg
     4801 gccaaagaga tccctgcggc caaaggtgcc gccggcaagt ggaaagccgt caaaggcaag
     4861 ccggtcgcgc ccactgcgaa tgccaaaacc cacgcagcgg aaaaagcggc tcccgaaaag
     4921 ctcgcgccag aaaaagcggt ggccgaaaaa ccagcccccg aaaagctcgc gccagaaaaa
     4981 gcggtggccg aaaaacccgt cgccgcgccg cccaagaccg ccgaggcacc ggcagccaag
     5041 cccgctgtgg cccagcccat cgcccaacct gcgcctgtcc cgatgcctgc acccgcgccg
     5101 gctcctgcgc cagtcgaagc gcgtccgagc gggccggagc ttcccgcgtt tgtgaacctc
     5161 gagcagggtc gtcgcgcgct gctcgcaggt cgcaactacg aagcggcgct gtttcttggc
     5221 aaagcgtatg aacaaggcgt cggagaaccg ctggccttgg aatggctggg cgacgcgatg
     5281 cgccgggtgt cttcgcttga aagtgcgctg gtggaacagc aggcggacct caagctggcg
     5341 cgctttttcc caagtggcca gcgcatcctg acgctcagtt ggtttggttc gctgtcgctg
     5401 tgggatggga cgaccggacg acggattccg cagccgtcgg tccccagcca gcccatcgtc
     5461 gacgcagtgc tgtcaccgga tggcacgcgg ttttattcga tcgacaaccg cagccaagtg
     5521 cagatttggg atgcggaacg tggtgcggtc ctggccagtc tgccggcgat tgatgtcagt
     5581 gtgacccggc tcagcagcga cggacgaaga ctgctcacgg tcagccgcgg cgcaagtccg
     5641 accgatttga tccagctatg ggacatggcg acgggccgca tgctcgccga gctgcgcggt
     5701 gccgatgcag aaggcaccac cgacgtccgc tttgcccctg gcgctcgcta tgtgcttatc
     5761 gaacggacgg aagcggtgca caagatccgc gtctgggaca ccgtcgaaaa ccggatgaag
     5821 ctcgtcgaag aaggtcagcg cgtccagttt tcgtctgacg gtcgccgggc agcagtgttt
     5881 ggcagcggaa cccggcccca gcttttcgac ctgacgacgg gcaaaaacta cgcccagctt
     5941 cgctaccagc aaaccgcgat tgtggaagcg cagttttctg ccgacggacg ccgcgccatc
     6001 accatccaca aaagcaacga ttccgacggt gaaggtgcgg cccaggtggc ctgcatttgg
     6061 aacgtgaccg aagggtcgtt tgtcgcggtg atggcggaaa agaaaggcag catccgccaa
     6121 gttgtgcaaa gtcccgacgg caagctggtc gtgaccatga cagacaccgg cacggcgtcg
     6181 ctgtgggaca tcgacagcgg tcgaaagctg gcagttctag acgacgacga tgaaaacgag
     6241 gaaggcaaga ccaagctcgc gtttggtcaa agtggccgtc acgttctgat ccgcaacccg
     6301 agcagcctcg gtgtttttga actgccgagc ggtcggcagg tccagctttc cggcaccatc
     6361 gggacgctgc aagatgcgtc gtttagtcca agcgggcttt atctggccac cgtcagcacc
     6421 gatggaactg tgcgtttttg ggtcacggcc acgggtcagc aggtcggcgt catcaaccaa
     6481 ggtggcaacc ccatcaccga cgcacagtgg agccccgaaa gcgcctactt tggcacaatc
     6541 gacaagaagc accagctcac cgtttgggat gtcgcggagt cgaaacagcg cttttccatg
     6601 ccgacgccgc ccagcacgac caacctgtct tttggcagcg acgtgcgcta tgtctcgttc
     6661 gtcgatacgc agcagcggat ctcgattcgt cgtgtcgaac caggcgccac cgccaaaacg
     6721 acacgctcgc tgagcgaagt ggcgacgggc catacggtgc tgccactgac gatgcaaaaa
     6781 gcggtcgatt cgcagtttag cagcgacgga caaaggctgc tcgtgcgcga cgcgggtcag
     6841 acgtttttgg tctgggatct gcgattggga aaacagctcg tgcggctgca aggacacgcc
     6901 gacagtcccg acggacgcag attcagtcct gacggacgat cactcatctt gatgcatccc
     6961 gacgggaaaa cccggctgca tgacgcgctg accggcaaag cgctcgccga agttccgcaa
     7021 ggttcgctgt cgttttcgcc aagcagtccg cgcgtggcga ttggcgacgc acgcggtcgc
     7081 gtcacggtgt gggatctgac gcacggacgg atggtcagcc aaagcaccgg actcggtgcc
     7141 gagctgcgca gtgttcgctg gcttcccgac ggcaaacgga tcgccgtggt cagtgacttg
     7201 cccattgcgc gagtgtttga tgccaacacc ggtcgcaccg tcgcggagca gcgtcgcctg
     7261 ccgccaaatg ccgccgaagt cgccgtttcc cccgatgcca aatatgccgc agtagcgatt
     7321 cccgcgacgg cgaaaatccc cggcacgatc aagctgttcc catttgacgg cagcgcgccc
     7381 attcaagttc agcaaggcaa aacatcgacg ccgccggtgg tgttcgactt cagccgcgac
     7441 ggcaaacagc tgtttatcga agcacaaggc aagagccgct ggctgcgacc gattccagct
     7501 ggcgatgcgc tcgtcgaaca gctcggcggc gtgtttgcct ggaacgacga cagccatcgc
     7561 atctttagcg cccagcccga gatggccccc gtcgtcatcg acacccagac tggcaaaaaa
     7621 acctgggaac tgccgtcggc gatgcaggac tttcagaaag tgcagtttgg acccggcgga
     7681 acccatcttt tgtccgtaca caacgacgac tcggtgcgaa tctgggaaac agcgacgggc
     7741 cggctggtga tgtctttggc caaaccacag agcccgctgc gcgacgccgt gctaagcccc
     7801 gacggcaagc ggctgttcac cacccacgac gacgacgcga tccgccagtg gcacgtcgaa
     7861 accggcaagc tggtcgccga gctcaaaggt ctggccggcg cccgcatctt gataagcccc
     7921 gacagccgga cgctgttttc gaccgacgac agtggccgcc acatcgtctg ggacattcgc
     7981 gccgagcagc gcacgccgca ggaagtcgcc aagtggctgc gctgctacgt gcccttccgt
     8041 ctcgacggga ccaagctggt ttctgccgct gcaaatccga gcgagtgcaa gtaaatccgt
     8101 ctgcacaaaa gccccccgca ccgacagcga ttcgctcgcg tgtgacaaac cgcgcacagt
     8161 ttcggtaacg tgccggggtg ctgtcttttt ctgtcactcc gtttcatgag ctttcaccgg
     8221 ctgcgctgta cgaacttttg gcgctgcggc agttggtttt ttccgtcgag cagaactgcg
     8281 cctacctgga ctgtgacgga aaagatcaaa aagcgctgca tctgctcggg tcaagcgacg
     8341 atggacggct ggttgcctac gcacgcctgc tgccgcccgg tgtctgttat ccggaagcgt
     8401 cgattggccg cgtcgtcaat caccccgctg ttcggcgcag cggctgcggt cggattctga
     8461 tgcaagaagc gatttcgcag cttgtccggg cctttgggcc agttcccatt cgcatcggtg
     8521 cccaagcgta tttgcgtcgt ttttacgagt cgtttggctt cgtcaccgtc agtgatcctt
     8581 acgacgaaga tggcattttg cacatcctga tgctgcgcga aaaagaccag gctccgctgc
     8641 aaacagagcc ccacgatttg gcccgtagat agaatcccaa acgaaggaat cacgacatgc
     8701 agatttccca tcagacacca gatttcgccc gcagcgcaga ttcctattct tttggtgtat
     8761 accgtcgagc cattgccaat cccaacgtct gtgcatcagg atgtttggct ccgctgcggc
     8821 tgaaggaatg gcactacgtc agcgtgtcca ctccgcgcca tttttttgcc tgcgcagtgg
     8881 tccgcttggg atacgccgcc aagatgtttg cgtatctggt cgatcgtgaa catcccgatc
     8941 gcaccgtcga ggcaagcaca ctccatccgc tgagtctggg cgtccgtttt gcatccagtt
     9001 cgatcgcggg ccgcacggtc tggcacaaag gaaatcgatc gcttcagatc gagtatgttc
     9061 agagtccgac acctggttgg aacctggatg tgaacctgga actcgggccc gttgtgctgc
     9121 agggacagct tcacatcgaa gatgccgaag cgctggccct tctgtataag ttaccgacgg
     9181 gaaatccggc atatacccac aaagctgccg gtctattttt gcgcggaaaa ctgacggcca
     9241 atggtcagcc cattgatcta accgatgcga tggcaggaat cgactggacc cgaagtgtgg
     9301 cgcctcgcca aacccgctgg ctgtggagtg cactgcaagg agtgacgccg cagagacagc
     9361 gagtcggtct caacttgtcg gcgctcgtct acgatgatga aaacgggaat tcccaagaaa
     9421 atgcgctgtg ggtcgacgga aaagtctatc cgctgggtgg tgtaaagttc gttctgccca
     9481 aagacccacg caaacagccc tggcgaatcc acagccgatc cggcggaacc aaagaagtcg
     9541 atcttctctt tacgccgctt ggcacccgag aagaacacgt acacttggga cttatcaaaa
     9601 gcgactttat ccagccctac ggaacctatc aaggaacggt ctgtccgacg gattcaggaa
     9661 ttccttcgct gtctgtggat ggacttttcg gcgtggtgga aacccatgaa agcgtctggt
     9721 gaaaagaaaa gacgccgagc aaaatgacag cgaaacagac ccgctctggc agcgatttgg
     9781 gaatcggtta ggaatcattt aggaatcggc tcggaatcgt ttaggaatcg gcaaacagct
     9841 cgtcgcacag cgaaattccc agaccatccc cttcaggcag ctgaagcatc gccgattttc
     9901 cgtgatacgg actgtgcttg gtgaagcgcg aagtcaaaaa gaacggcgtg tccagatcac
     9961 aaacagaaat gattccggtt ccgcacaccg catgcagcgc cgccgtcatc gcaatctcgc
    10021 tttcgaccat gccacctatc atcagcggaa tttttttccg atggcacagc tcggcgattt
    10081 gcagcgcttg cgacacgccc gacttcataa acttgagatt caccatccca gctgccgacc
    10141 gatgcagtac atcgaatgca tcgtcgatgg tctgcaccga ttcatcggca cacacgggga
    10201 tcgggagccg ccgcgcaagc cgcgccatcc catcgagatc cgcagccgga agcggctgtt
    10261 caaaaaacag gggcacaacc tggtggcgca caagcgcttc gcaaagcgac agcgcggacg
    10321 cttccgaaag accctgattt ccgtcgagac tgatgcgcag actggccccg acgatgttcc
    10381 gcagcgccat cacccgttcg acgtcatcgg ccacactgcc gccccccact ttgaccttgt
    10441 aataaggaaa gtgatggtcg gcaaactgct ggtaaaagac cggcacttcc gacggggaaa
    10501 gccgcggcaa agtgatgtct gtttcggctt gtgaccaaaa tgcccggccc cacagcgcgg
    10561 caagcggcag gccgagctgc cttgcgtgca gatcccacag cgcgatttcc accccagcga
    10621 gcgccgtcgg tgtctgcgaa aattccggcc acagatcacg aaccaaccga tcgagcgcag
    10681 ctgccggcgt cagacccgaa agctggtcga caatcgcgcg gcaagccggc agtaccgtct
    10741 gctgggtatc atgcgtgagc accggaaacg gcgccgcttc ccccagaccg actcgtccgt
    10801 cggcatcgcg cagcacgatg agaacgtttt ccaacagctc gaccgagccc tgcgaaatgt
    10861 gaaacggcag gtgaagctgg gtcgacagcg gacgcacctg cgcggcgatc agcggacaaa
    10921 gcttcatcgt ctttcccaaa cccccaatgc cttgtcacag cgtatcagca gtccggaaag
    10981 aactcgtgcg tgaccgcagc gccgcgggcc cattgatcgt cgccgccggg caaaaggacg
    11041 ccgtcgtggg caatcccaat caccgatgcg agaaactctg cgtgataaga cagcgtgaaa
    11101 caggcttttc ccgatgactg gcccgtcgct ggatttttgc gcaagtcgaa cgcattgggc
    11161 gcaaatgccg cgatctcttc gtcgtggagc ttgcagacca ttttggccag cgcagccaag
    11221 ttgatcgtcg acaagttcgc gttatacgac ggatcagcct tcagcgtcca cggtggcccc
    11281 gacggccagt cggcaaaaat tcccagccac ggcgcgccca gcccgtacag ccgccgcgat
    11341 gaaaagtgaa acgcgagctg atttatcgag ctgccccaga acaccgtctt gcgcaaaaac
    11401 gccatacagc tgccgccgtc ttgctgcgtc ggatcttcgg ggtgataaca ggcgtcaaag
    11461 cccggtgtga cctggccact cgacgtgaca aacttgttaa aaacgtagtc ttggccgcgg
    11521 tactcgctcg cggcgtacag gtcttcgtaa tcgaggctgc gcccgcttgt tgcaaactga
    11581 atcgacgcgc caccgacttc gatgatcgca aagctgtctt tggggcttgc cggaacctgc
    11641 tgcgccacgc caagccacgc cagccgcgct tccatcagac cgctgatcgt cttggcgacc
    11701 acatgttctt gcttcgcttc ggcatgaaag aagcgctcga tctcggtcca gacggcctta
    11761 aattcctgcc tgtcgtcggg caggttggtg ctcggattgc gaaaaccgcc cgtccccagc
    11821 gccaccacgc cgcgaatccg atcccgcagc tgcggattgc cttttctgag cggatcacca
    11881 agcatttccc acgcttgccg cagccgcggc gcgataagac ccggaacttc gtcggccttg
    11941 tgcccacgca cgagggccgc gatgccaccg ctcatcttca cgcaagtcgg attggcgccc
    12001 gcatttggaa tgcgacaccg ccgtcgctca gcagcgctgc cgtccacgag cacgggatac
    12061 atgcagaagc gagtccccga cgatcccaag tcgagaacca aatgcaccga ctctgtcttt
    12121 tccgtcggac ggattggttc tgtgtgcgcg ccttcctggg gaaaccctaa cagcgaaagg
    12181 aagcacacaa gcgggaccgt cgagaaaagg cggagtggac gctgacgcat gtcgcctcca
    12241 tagaagtgga gcagttaaca aaaaacgaag cgaagccgag cgggaggcag gcagccgcag
    12301 ctgcgaacag accaagaagt aacccaacta ccgacaagta gggttagctt tttgcagttt
    12361 cgtttggtca aaatgtagtt tttgtagcat tcccatttgg cgcatcgggc tgcgcaagcg
    12421 ttgtgtcgcc aggcgatctg ggcgatagta atggttgcgg cagcttgtcg ctgccgagcc
    12481 gaccgggggc tacatgccga cgcacgaaga gcagcttcgc cgcgagcagg aactcttcga
    12541 aaaaaaagaa gaggcgcaga agcattcgca actgccgcaa catgcgcagg ttcacgagca
    12601 gagcgagcgg ctgcttgccg aagaacagcg cgtcgagcag tcgcggctcc tgtccagtct
    12661 gaaggccgag gcattttccc caagccagct cgccgagctg ccgaagcaga gcgagccaac
    12721 gaaagcagcg gtcgagcagc tgttccatca tctcgtcgag aaaaaagaca agccgccgac
    12781 ggacgcgatc aaaaaaacgc agcggacgat gacccaggtc gatcgcaaga ccggcctgcg
    12841 ctcgcccgag ccgaaacacc gcgaacctga cgatctgttt gccacaggaa tcgaaggtga
    12901 tttcgtaccc aatacgaaga tttcggcagc ggactttgat gtccatccaa gccccgacga
    12961 tggaatcgac tgggggggaa gtggccctcg ccgtcgcaaa cggggacagc ccagcaaagc
    13021 cggtatcaaa aagaagcctt cgggaagcga caaatagcgc cgccgaagcc aaacgatcaa
    13081 ggagatgacg tcgccgatga gcgcacaaac cgagtctgag cggcaagccg ccgagcagtg
    13141 gctagccgcc gagctgaatc gcatgtggct ggtcgccgag cgctgtctgc gccaattcca
    13201 gcgcgcccag ctgcgaccgc agccggacaa cccgtcgctc gccgaggtcg aaggcatctt
    13261 caccgctcgt cgggcggcgc gtggtggtct gtcgagcggg ccaatggatg aagccgagct
    13321 gtcgcgtgcg atcaccgatg tcgagagttc gctttatgcg ctccgacggt ctgcgccgat
    13381 cggacgggtg attgaaaacc tggggctgtc gccgctggaa gtcgaaacgc tgatcgttgc
    13441 gatgtcgccg cacatcgacg cgccgctgtc ggatgtgttt tcgctgctgc gcagctcgcc
    13501 aagtcgccgc ggcgtcgatc tttcgctggt ttcacagctg tatcagctcc ggcggagcga
    13561 ccggctgcag ctgctggatg cgctcgaccc cgagcggccc cttttggcgt ggcggatgat
    13621 ccagatcatt tcgccagagt cgatggaaag ctatagctcg gtaaactatc gcgccatcat
    13681 gccgacgttt gacgtgttgt cgacgctgtg tggccgtggt gaactgtcgc cagcgcttgc
    13741 caaaacatcg aagcttttgc gcgcggaaaa acgtctggat gatgtggttc tcgacgagca
    13801 gctgcgccaa aaaatggaac ggctgtgcac gatggcggac cacagcgttt tgcagcagca
    13861 accgcagcac atgccgtggg tgatgttcta cggtgccaaa ggcagcggca aaaaagagct
    13921 ggcgtcgcgg ctagcggcct actgtggacg atcgcttttg tggttcgatc cgacgcaggc
    13981 cgaacaggga acgctcgatg acctcctgcg ccgtgcccag cgcgaagcct tgctgcgagg
    14041 cgcggtgctg tacattggcc cgctgcgcgg cgacttgcta aacgacaacg ggcgcgaact
    14101 gataaagcgc atcatcagct atccgtcgat gctcgtgctc ggtgtggaac agacgcagcc
    14161 gccgcgcatg accatcgacc acagcatgca ggaagtgcag ctttcgattc cgcccgagcc
    14221 gtcgcggctg attctttggc agcgggtggt gcccgagtcc attcgcgcca aggatttgtc
    14281 gctggaaagc atcgctcgcg cctttcacct ttcgcccggc gaaatcatca acaccggcga
    14341 agaagcccaa gagctggcat cactgagccc gagcgggacc gtcacccacg atcagctgcg
    14401 cgcaggcatc gaccgcaggc tgcgcaacga tctgggcgac atggccaagc gcattgccgt
    14461 ctcagctcgc tgggatgatc tggtgctgcc cgacgatgac atcctgcgga tcaaagagtt
    14521 tattggccgc agcaagtatg cctaccgagt gtattccgac tggggattcg gaatgcgcgt
    14581 cgggtacggc aaaggcatga tcgcgctgtt ttcgggaccg ccgggcaccg gcaaaacgat
    14641 gctcgccgga ctgattgcgc aggcgctgga ccttgatgtg tatcaagtcg atctggcgca
    14701 agtggtcagc aagtgggtcg gtgaaacgga aaagcaactg gccaaagtct tcgacgctgc
    14761 cgaacgtgcg cacgcggtgc ttttgttcga cgaagccgac tcgctgtttg ccaagcgtac
    14821 cga
//
LOCUS       DAORGN010000126        14811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1668_length_14811_cov_5.740716, whole genome shotgun sequence.
ACCESSION   DAORGN010000126 DAORGN010000000
VERSION     DAORGN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1668_length_14811_cov_5.740716"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKL17_13875"
     CDS             <1..883
                     /locus_tag="PKL17_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45872.1"
                     /translation="CGIEPMQPVGELVDRLESLEERLSGAAAARPRPAPSGGPGAPSG
                     EDPRPFAASTGLSTPRSAAPSAPVGASTERPLSPPASAGPASAAKPPTTSHVAATGAS
                     QAAAVAVAPAVAAIASVAEHVAPVVPATIPAIESEPAAAPAPPAPEAAPPATLSPPGE
                     LPDFHTALNQVMAQDPTLVVLHEARKLHWQRDKIVLGFPKEFFLDSIEKKRADAERVL
                     SAICGCEVSLSVQLDQGPRSADPSRWSLRETHEQQSSDEKASRKREAETHPARQLLSQ
                     AFGAVSFVAPTLLDEVS"
     gene            885..1238
                     /locus_tag="PKL17_13880"
     CDS             885..1238
                     /locus_tag="PKL17_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNK45873.1"
                     /translation="MSDPNNPFGSIPGMPDFGQIMQQAQRMREELVRSHDSLGTKTVE
                     AGAGGGMVSAVVSGTRELVKLRIDPQVIDAKDPQMLQDLIVAAVNQALQKAKALEEEE
                     MRRTAMGMGLPPGML"
     gene            complement(1257..1454)
                     /locus_tag="PKL17_13885"
     CDS             complement(1257..1454)
                     /locus_tag="PKL17_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45874.1"
                     /translation="MEKPDELSASDAQAAFLAGVSKRMLQDAVYVCAIFQLMTRLADT
                     LDFSVKGADGSKFLLRFGYRL"
     gene            complement(1584..1829)
                     /locus_tag="PKL17_13890"
     CDS             complement(1584..1829)
                     /locus_tag="PKL17_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45875.1"
                     /translation="MRLSILDRPHGMWQRAKLAIIRRVAGGTAPDPVKVILFRYDWFG
                     AHLVRLLQAVLRGDSEWSAGERELLAAFTSKLRKCEY"
     gene            complement(1830..2153)
                     /locus_tag="PKL17_13895"
     CDS             complement(1830..2153)
                     /locus_tag="PKL17_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45876.1"
                     /translation="MNSVLGKGRHTIETQRRAVFDRQFDTIDEPLRQLLGKTESAAYR
                     VTDNDFAPLATDREGNQDSLFELIVSTAIGAADRSLRTGLAAIEQAERGATQAGDSAA
                     SKGVS"
     gene            complement(2319..3038)
                     /locus_tag="PKL17_13900"
     CDS             complement(2319..3038)
                     /locus_tag="PKL17_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK45877.1"
                     /translation="MAAHTLEKSRLILDVLIPALNEEAAVGQVVTALKALGHPVRHVV
                     VIDNGSIDGTASAAQKAGALVVKEQTRGYGAACLRGMAFLSKQEVKPDVVAFLDADGS
                     DDAQDMSRMIDAIVSGADLVVGSRTLGISEPQALSTAQRVGNAVATTLIRAVYGQNYT
                     DVGPFRLVRYPALLALGMTDEDHGFMIEMQVKAVRRGLRIVEVPVRYRPRIAGESKNV
                     KGSLAAGRKMLFTILRHSTQR"
     gene            complement(3019..3894)
                     /locus_tag="PKL17_13905"
     CDS             complement(3019..3894)
                     /locus_tag="PKL17_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HNK45878.1"
                     /translation="MRTSKVDAPQGAKGGGIFGTGSDENTLHVYAYAYAATFRLRDLS
                     PVFGDAEVEHDKDCLVARFSGVKTRMAVLFDFGSVVLIGLSREERERLISAIGARIAP
                     EPHPPLTEDFLVEVRPGAEIEVQFDRVILSEASVSALKVISLVLAQSAAMDYYDEDVQ
                     EIQQRTHVITRALQAEGRVPGRLDKLVQFIGSCIETKNGMIATLALFDKPDATWDNQQ
                     IDRIYSGLRVELEIDDRFRALEAKLRMIQENLVLLADLYHSRSTWRLELTVVLLILFE
                     ILLNLWNLFRGGTHA"
     gene            4011..4400
                     /gene="gloA"
                     /locus_tag="PKL17_13910"
     CDS             4011..4400
                     /gene="gloA"
                     /locus_tag="PKL17_13910"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNK45879.1"
                     /translation="MRLLHTMLRVGNLDRSLAFYTEVLGMKLLRRKDYPDGRFTLAFV
                     GYGEESSHTVLELTHNWDTESYDLGKGYGHIAIEVEDAYRTCEEVKARGGKVAREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKNSH"
     gene            complement(4437..5318)
                     /locus_tag="PKL17_13915"
     CDS             complement(4437..5318)
                     /locus_tag="PKL17_13915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNK45880.1"
                     /translation="MFLRSQTASTGSSFSSHNARPLSPSRRFLSIAALLSGFTLSVLA
                     QGCGETLETDTVMRGIVNGTDDTQNRYPNVGAMVVYMNGQWTALCSGTLVSPTVFLTA
                     AHCTGYVVQNNLLPAYVSFDPLSINGTTPFLPATVYHNPGYNSSMSDSQDVAAMVLAT
                     PITNIQPAQLPTANLYDTTDLHSAEFVAVGYGGNYRTTGGGSGNYWNYDGTRRYASQS
                     YRNIHDAWIYLSQNDSQGNGGTCYGDSGGPNFIGQGNVIAGITVKGDSACRSTNDIYR
                     MDIANARNFLSQFMALP"
     gene            complement(5579..6652)
                     /locus_tag="PKL17_13920"
     CDS             complement(5579..6652)
                     /locus_tag="PKL17_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45881.1"
                     /translation="MKLRRLLGTVFVVGLIAYGWKASGDLRPFVKIDPVGVQNCRVVQ
                     TEGLIGAEDMEFDHQTATLYIAASNRRKSGNFRPDPGAIFRWQPDKEPAPVRMDVQGN
                     DDALRPHGIGLYRHPGGERRLFVVNHTSRGETVSIFRIETANGIEKLATIREVRAPQF
                     VSINDVAPSGLESFYVTNDHGRPPVLGHIWEDFAMLSRGSLVYFDGTSARTVVSGLRY
                     ANGVTLSADRKLLTVAETTAYRIKFFHHADDGKLQLISERSIDTTPDNFSQDERGEFL
                     IGAHPSPYQFIRYAASASDPSPSEVLSMHVTPDGSSQTFSTVLRAPGEVFGASSVAAA
                     HGPHLVVGGVFDRGVLYCAKPAS"
     gene            complement(6674..9538)
                     /locus_tag="PKL17_13925"
     CDS             complement(6674..9538)
                     /locus_tag="PKL17_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45882.1"
                     /translation="MLSRAARMLGLVLPLVVSSSLGLASPPQKPMQQKPSPTAKDTEP
                     VQPAFRFDGPLAQIQHALRSGKPSDAQQLAEKLRPTTKDAKVRDDLEALFAQSLCLQG
                     DVPQAIQKLEPFVKDHPQALASRLALGQAYRQSGQRDKERAIWNGFFDDHDADKLNLQ
                     DPRSVRLLGLAAHYLGVYQDAYEQLTNAVALAKEKKLTEEFVLASLALSTLRIEKYEV
                     GHAEESTHDALRRDSQNPDAKALMARIKLEQGNDVTEASELIDETLKTNPIHPAALAI
                     KAEILIDNEQYEDALAILDPAIARNGYDLPARALRAAALLLLDRQKEYEAEKAETLRR
                     SPLFSQFHRIVSDRLQIQHRYELQVTVLEEAVQLQPKDYYALGDLGRSYLQLGDDERS
                     YQTLLKAWKGDRYNRKTFNLLELFEKYHQKTYKVLTVDIDPQKPGKGGLRIRVHKDEE
                     ELLIPILKPLVQAEYVEFSRRYGFSPKLPISIELYKEPDNFSVRTFGLPSADPGMLGV
                     TFSRVVTGRSPSQGKLPWGLMVWHELGHVFAIELSNGKVPRWFTEGLSEWETMQIQPS
                     WSRRTHAEVAAALRDDRLLSMADLNIGFTRARSQSHVVVAYHQAALSVAFLAKRFGFP
                     KIVEALKLFGAGKRTKDVLQQISGMPIAKLDEEFRSDLRKQLAAYDGTFFIRPSDYAD
                     GEGLRQKIRQSPQNAQLQGLLAVAMARSGADPAETAGQIAVARKLDPRCKEAVLADAE
                     LQQKLGRRSEAEKLFRELISIGGDGFDVRQRLGDLYTAEKKFPAAMAEYERAKKLDPD
                     RSEPYESLAALYLQDKKESEALRELSVAAGLDFGNDKVLIQLLDGYANKKQTKEAIKY
                     ADMLIYIAPYREKSRLQMAEAWLQLGQPQRAALHLDAAKRLLPDAADIEEDELPKLQA
                     ESARVEELRRQLAKTKSQPFSGPSTLLDVARQKAGLPLVP"
     gene            complement(9578..10933)
                     /locus_tag="PKL17_13930"
     CDS             complement(9578..10933)
                     /locus_tag="PKL17_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNK45883.1"
                     /translation="MPQPASTPSLLRSVVSTLATRFAILPLGLIQGALTARALGPDGL
                     GRYSAVLVDVNIVTTLLGLGLPGGLAVLTGESQGQPRAVRDLWWKGIRHVLLALCLAS
                     LVGIALARLLPSQFFRFLTDRTPLVTALLILCVVGQLGRDIHNALLWGSQRFASQNRL
                     SLALSVFQLASVAGLFLWKKLALETALGLQALSLWGLCLAFLLRVFFDSHNKAVSFPK
                     TPEHSDVPSTKRLYGTSLRNFFHILPDMLLLRIDVYLIELFLPPLAMRHQLGLYQAGA
                     RVAELILMLPGTLNAVLFAKAAAKEDIGEQALLSAKLGLYLGLGSCVAMFFIGKPLLL
                     LFYGDRFAGSFLPCLLLLLGCSALCFSGPLAGALSGARGYPPSVILAQTLALVTNVAL
                     NLWLLPRYGIIGAAWASVAAYTVSALCISIAFARHFALPLRRLIAWVPPHTIYHRLAR
                     R"
     gene            complement(11013..12110)
                     /locus_tag="PKL17_13935"
     CDS             complement(11013..12110)
                     /locus_tag="PKL17_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45884.1"
                     /translation="MTSLSVAVIGGGRWARALASFLLHNQRRSPERIARVLHYRPPRE
                     LIRAGQEDSRAEQEASVHHTDVPAAAGEVPDVHAVHSAPSAERAQVTSIVLSDLAAAD
                     VIFMAVPAASVRKVLRLAAPHLHGNQSMIHAIGSVIPASDECPQSQLISRAIEEETPI
                     RKIGVLAGPALAPDLEEFAPAALVCGSRFDEVGAQAIQVLSGHSLLVYTTRDLIGVEL
                     ARAGSSVIALAAGVANILDLGTPARAILITRGAAEMARIAVQLGADERTFFGLAGVGE
                     FVAATERRGSADFELGRLLGQRIPLAEALRQVGRVCDSPQMVREAHLLGHRNHVRTSI
                     VSALYQLLHGKFDTKDALLALLSTDVHNERE"
     gene            complement(12107..13729)
                     /locus_tag="PKL17_13940"
     CDS             complement(12107..13729)
                     /locus_tag="PKL17_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNK45885.1"
                     /translation="MSGFQPVRDYPLLRFHKERDNIVKETTRRALQTVLLTSPPEYVL
                     NDACLQEIRRLSESPAQEEVRSPSEWKRLWRSIGGKSRTTLEHDLERLIADYANDVAG
                     GFNKQVYTFASQVVPPALSLLLSPESPLKLPLHMLQPTALLQQLGERLQLQGPLDKLR
                     ALAPHGTMVCVPTHLSNLDSPVMGYSLQAAGLPPTTYGAGKNLFTNPMLGFFMRNCGA
                     YRVDRRIRHELYKDVLKTYSQVILERGYHSMFFPGGTRSRSGRVENKLKLGLLGTALT
                     ATVEGFRRQAGLVAGVPVRPIFIVPVTINQQLVLEAETLIADYLKETGKARFIIEDDE
                     SAQWRKVVDFVRSTMTHTGAIVIRFGEPMDVLGNPITDSGESLGPHGMVVDLARYFWS
                     GGKPAHVPQRDAEYMNQTGLAISRAYRRETVLLPTHLLAYVLWRRVCVALPGLDVYRR
                     LRQPLDLHVPAQELLADMERLQNHLRNRKHHLGPRADGSAEQVVDAALTAFASYHTEP
                     AAIRTPSQAVTVGDRQLLYYYQNRIESLGDEL"
     gene            13886..14716
                     /locus_tag="PKL17_13945"
     CDS             13886..14716
                     /locus_tag="PKL17_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45886.1"
                     /translation="MLHSHLKNGLHDIFHHFDSNHSTHPAPHHPAPHPAPHHPAPHPA
                     PHPAPHHPAPHPAPHHPAPHPAPHHPAPHPSPVSHWTDHGHVGDPSGILDHIHVTGAG
                     SRTARAEGLRHGGVAASQTIAARDTHRVLDQHNLRHEYESVGQEHHIPPALLAAIASR
                     ETHGGSALARNGTGDHGHGYGVMQVDNRHGQHPDRSQGPYGHAHLEQAAGIFDHKLAA
                     VRHQFPHMSPEDQMRTAVSRYNGGRGLPGSRSDVGTTGGDYGNDTIARAQYYAHHWAE
                     "
BASE COUNT         3042 a         4405 c         4353 g         3011 t
ORIGIN      
        1 gtgcggaatt gaaccgatgc agcccgtcgg tgaactggtc gatcgactgg aatcactgga
       61 agagcggctc agcggggcag ccgctgcgcg tccacgtcct gcgccgagcg gtggtccggg
      121 ggctcccagc ggagaagatc cgcgcccttt cgccgctagc accggcctct ccacgcctcg
      181 gtctgctgcg ccgagtgctc cggtcggtgc gtctacagag cgtccgcttt ctcctcccgc
      241 gtcggcagga cctgcgtcgg cagcaaaacc acccacgaca agccatgtgg cagcgacagg
      301 cgcttcgcaa gctgcggcgg ttgctgtggc cccggcagtg gctgccatcg cttctgtcgc
      361 ggagcatgtc gcgcccgtcg ttccggcgac gatccccgcg atcgaaagcg agcctgctgc
      421 cgcgcctgcg ccgcctgccc ctgaagctgc gccacctgcc acgttgtccc cgccgggtga
      481 gctgccggat tttcacacgg cgctcaatca agtgatggcg caagatccga cgctggtcgt
      541 gctacatgaa gcgcgcaagc ttcactggca gcgggacaag atcgtgctcg ggttccccaa
      601 agagtttttc ctcgattcga tcgaaaaaaa gcgggccgat gccgagcggg tgctgtcggc
      661 catttgcggc tgcgaggttt cgctttcggt gcagcttgat cagggtccgc gatctgccga
      721 cccgtcgcgt tggtcgctgc gcgaaaccca tgaacagcaa agcagcgacg aaaaagcgtc
      781 acgcaagcgc gaagcagaga cacatcctgc aaggcagctt ctctcccagg cattcggtgc
      841 ggtatcgttt gtggcaccga ctctgctaga cgaggtgtcc taagatgtcc gatcccaaca
      901 atccctttgg ttcgattcct ggtatgccgg atttcggtca gatcatgcag caagcgcagc
      961 gcatgcgaga ggagctggtc cgctctcacg attcgcttgg cacaaagacc gtcgaagccg
     1021 gcgctggtgg cggcatggtg tccgcggtgg tcagcggcac ccgcgagctt gtgaagctgc
     1081 gcattgatcc gcaagtcatc gacgcaaaag acccgcagat gctgcaagac ctgatcgtcg
     1141 cggcggtcaa tcaagcgctg caaaaagcca aagcgctcga agaagaggag atgcgtcgca
     1201 ccgcgatggg aatgggtctg ccgccgggca tgttgtaagc gcggcggtat cgcctgttac
     1261 agtcggtagc cgaagcgaag caggaacttg ctgccgtctg cgcccttgac cgaaaaatcg
     1321 agtgtatcgg cgagccgtgt catcagttga aagatcgcgc agacgtacac ggcatcttgc
     1381 agcatccgct tgctgacccc ggccaaaaac gcagcttgtg catcgcttgc gctgagctcg
     1441 tcgggctttt ccaccagttt ttccaggtac gccagcgtcg ctttcagcgg cggcgaaatg
     1501 tccgcgctgc gatagtccgc aagaaccgct tgggttcgat cttttccgat cagtgccgac
     1561 gcgaccgcgt cgtgatcacg gattcaatat tcacactttc gtagcttgga cgtaaacgcg
     1621 gccagaagct cacgctcacc tgcgctccat tcggagtcgc cccgcaggac agcttgcaaa
     1681 agccgcacga ggtgggcgcc aaaccagtca tagcggaaca agatcacttt gaccggatca
     1741 ggggcggtgc cgccggccac gcgacgaatg atcgcaagct ttgcccgctg ccacattcca
     1801 tggggtcggt cgagaatcga aagacgcatt taggacactc ccttgcttgc agcggaatcg
     1861 cccgcctgtg tcgcgccccg ctctgcttgc tcaatcgctg ccaggccggt gcgcagactg
     1921 cgatcagcgg ctcctatcgc tgtgctgacg atcagttcaa acaaggaatc ttggtttccc
     1981 tctcggtctg tcgcgagcgg ggcgaagtcg ttgtcggtga cgcggtacgc tgcgctttcc
     2041 gtcttgccca gcagttgccg cagcggttca tcgattgtat cgaactgccg atcgaacacc
     2101 gcgcgtcgct gtgtttcgat cgtgtgtcgg cctttgccaa gaacagagtt cacaagagcg
     2161 ctccacaagg gagcgaatcg atcgctgtgc ggcatggact ttcattttaa actgagtcag
     2221 cgcgcggcat cgaaaaagta atgcgacacg acctttgggg gaatcaataa gaggcatgta
     2281 aaagtcctca tattgatttg gtggaatgtg gaacgtcgct agcgctgtgt tgagtgacgc
     2341 aagatggtaa acagcatctt gcggccagcc gcgagactgc ctttgacgtt tttcgattca
     2401 ccggcaatgc gcggacgata gcggaccggg acttcgacga tgcgcaggcc gcggcggacc
     2461 gctttgactt gcatttcgat catgaagccg tggtcttcgt cagtcattcc cagtgcgagc
     2521 agcgccggat agcgaaccaa acggaacggc ccgacatcgg tgtaattttg cccgtagacg
     2581 gcgcggatca gcgtcgtcgc aacggcattg ccaacccgct gtgccgtcga cagcgcttgc
     2641 ggttccgaaa tgcccagcgt gcgtgaaccg accaccagat cggctccact tacaatcgcg
     2701 tcgatcatcc gcgacatgtc ttgggcatcg tctgaaccat cggcatccag aaaggcgacg
     2761 acatcgggct ttacttcttg cttggacaaa aaagccattc cgcgcaaaca ggccgcacca
     2821 tagccgcggg tctgttcttt gacgacgagc gcgccggctt tctgggccgc ggaagctgtg
     2881 ccatcgattg aaccattgtc gatcaccacg acgtgacgca ccggatgccc gagcgctttg
     2941 agtgccgtga cgacctggcc caccgccgcc tcttcgttga gcgccggaat caggacatcg
     3001 agaatcagcc gcgatttttc aagcgtgtgt gccgccacgg aacaggttcc acaggttaag
     3061 gaggatctca aacaggatga gcagaacgac ggtcagctcc agtcgccagg tgctgcgcga
     3121 atgataaagg tcggccagaa gaaccaggtt ttcctgaatc atccgcagct tggcttccag
     3181 cgcccggaag cgatcgtcaa tttccagctc gacgcgcaga ccactgtaga tgcgatcaat
     3241 ctgctgattg tcccaagtcg catccggttt gtcgaacagg gccaacgtcg cgatcatgcc
     3301 gtttttggtt tcgatgcacg aaccgataaa ctgcaccagc ttgtccagtc gaccgggcac
     3361 tcgtccttcg gcttgcagcg cgcgggtgat gacgtgggtg cgctgctgga tttcctgcac
     3421 atcttcgtcg tagtagtcca ttgccgcaga ctgcgcgagg accaggctta tcactttgag
     3481 cgctgacacc gacgcttccg acaagatcac gcgatcgaac tggacctcga tttcggcacc
     3541 ggggcgcact tcgaccaaaa aatcttcggt cagcggcgga tgcggctcgg gggcgatgcg
     3601 ggcgccgatc gcagaaatca gacgctcacg ctcttcgcgc gacaggccga tgagcaccac
     3661 cgagccaaag tcgaacagca ccgccatgcg ggtcttcacg ccggagaagc gagcgaccag
     3721 gcagtctttg tcgtgttcga cttctgcgtc gccaaacacc ggcgaaaggt cacgcagtcg
     3781 aaacgtcgcg gcataggcat atgcgtacac atggagtgtg ttttcgtcgc tgcctgtgcc
     3841 aaaaatcccg ccgcccttcg caccttgcgg cgcgtcgacc ttgctcgtgc gcatcgtttg
     3901 cggaagtaaa gcgcagcggc gcaaaaatta caatgtccgc cgtcctagac ctctggtttc
     3961 cgttaagatt cggcctcgca caccacggac acaggcaagg agaaacggcg atgcgactgc
     4021 ttcacacgat gctgcgggtt gggaatctgg accgctcgct tgcgttttac accgaagtgc
     4081 tggggatgaa gctcttgcgg cggaaagact atcccgacgg acggttcacg ctcgcgtttg
     4141 tcggttatgg cgaagagagt tcgcacacgg ttctggaact tacgcacaac tgggacacgg
     4201 aaagttacga cctgggcaaa ggctacggac acatcgcgat cgaagtcgaa gatgcgtaca
     4261 gaacctgcga ggaagtcaaa gcgcgcggtg gcaaagtcgc gcgcgaggcc ggcccgatga
     4321 agcacggcac gacggtgatt gcgtttgtcg aagatcccga cggttacaaa atcgaattca
     4381 tccagaaaaa cagccactaa gctgtgttcc ccatcagaac atgcgctttg gcaggactag
     4441 ggcagggcca taaactgcga caggaagttg cgggcgttgg cgatgtccat gcgatagatg
     4501 tcattggtcg aacgacacgc gctgtcgcct ttgaccgtga tgccggcgat gacgttgccc
     4561 tgaccgatga agttcggtcc gcccgagtcg ccgtaacagg ttccgccatt gccctgtgag
     4621 tcgttctgcg acagatagat ccacgcatcg tgaatgttgc gataggactg gctggcatag
     4681 cggcgggtgc cgtcgtaatt ccagtagttg ccgctgccgc cacctgtggt gcggtagttg
     4741 cctccgtatc cgaccgcgac aaactcggcg ctgtgcagat cggtcgtgtc gtatagattt
     4801 gccgtcggta gctgcgccgg ctgaatgttt gtgatcggcg ttgcaagcac catggcagcc
     4861 acgtcttggg aatcgctcat cgagctgttg tagccagggt tgtgatagac cgtggccggc
     4921 agaaacggag tcgttccgtt tatcgacagc ggatcaaagc tcacataggc cggcagcagg
     4981 ttgttttgga ccacgtatcc agtgcagtgt gctgcggtca aaaataccgt cggtgaaacc
     5041 agtgttccgg aacacagcgc ggtccactga ccattcatgt agacaaccat cgcgccgacg
     5101 ttgggatagc ggttttgggt gtcatcggtt ccgtttacga tgccgcgcat cacggtgtct
     5161 gtttccagcg tttcaccgca gccttgggca aggacagaca gcgtgaaacc ggaaagcagc
     5221 gcagcgatgg aaaggaatcg gcgggaaggc gatagagggc gagcattatg cgacgaaaaa
     5281 gaactgcctg tggacgcggt ttgtgaacgt aaaaacatgt gcgtgcgctc ctatgtgtga
     5341 ttgcattccc gccccttggt tcccccattc cccaaacatg gcagatgttc ctatcgtatc
     5401 gcggtgtcct tgggcgcgat agcgacattg cgacgcgaat gtgaacgcat gcacatcgtc
     5461 gcggaatcct tgtgtctgtg ttgtggaaac ccggtcgttc tgatcgcttg aacgggggac
     5521 tgacttcgcg tcgcggtagg ctgcttggcg gtgtgatgct acttgtgcgg cacaccgatc
     5581 aggaagcggg ctttgcgcaa taaaggactc cgcgatcgaa gacgccaccg acgacaagat
     5641 gcggaccatg cgctgcggca acgctggatg ctccgaacac ttcgccgggc gcgcgcagca
     5701 cggtcgaaaa ggtttgggaa cttccatccg gagtgacgtg catgctcagc acttccgacg
     5761 gagaaggatc gctggcggac gccgcatatc ggatgaactg atacggactg ggatgcgcac
     5821 cgatcaggaa ttctccccgc tcgtcctggg aaaaattgtc tggtgtggtg tcgatggatc
     5881 gctcgctgat aagctgtagt ttgccgtcgt ccgcgtgatg gaaaaacttg atccgatacg
     5941 cggtggtttc cgccacagta agcagcttgc gatcggcaga cagcgtgact ccgtttgcgt
     6001 aacgcagacc gcttacgacc gtccgtgcag atgtcccatc aaagtagacc agcgatccgc
     6061 gcgacagcat cgcaaagtct tcccagatgt gtcccagaac gggcggtctg ccgtgatcat
     6121 ttgtgacata aaatgactca agtcccgacg gtgcaacatc attgatgctg acaaactggg
     6181 gcgcgcggac ttcccgtatg gtcgcaagct tttcgattcc gtttgcggtc tcgatgcgga
     6241 agatgctcac cgtttcaccg cgggacgtgt gattcacgac aaacagtcgc cgctctccgc
     6301 caggatgtcg atacagaccg attccgtgtg gtcgcagcgc gtcgtcgttt ccctgtacgt
     6361 ccatccggac aggcgccggt tctttgtcgg gctgccagcg aaaaatcgcg cctggatcgg
     6421 gacggaagtt tccgcttttg cgccggtttg atgcagcgat gtacagcgtt gcggtttggt
     6481 gatcaaactc catgtcttcc gcgccgatca atccttccgt ctgcaccacg cggcagttct
     6541 ggactccgac cggatcgatt tttacaaaag gccgcagatc accgctggct ttccatccat
     6601 aggcgatcag accgacgaca aatacagttc caagcaagcg acgcagtttc atcgaaatca
     6661 ccacgatgta gtgttatgga acaagtggca gaccggcttt ttgccgcgcg acatcgagca
     6721 gcgtgctggg tccagaaaag ggttgggact tggtcttggc aagctgacgg cgcagctctt
     6781 ccacgcgggc ggactctgct tgtagcttgg gaagctcgtc ttcttcgatg tccgccgcgt
     6841 cgggaagcag gcgttttgcc gcgtccaggt gcaaggcggc gcgctgcggc tggcccagct
     6901 gaagccacgc ttctgccatt tgcagtcgtg atttttcccg atacggcgcg atgtagatca
     6961 gcatgtcagc gtacttgatc gcttcttttg tctgtttctt gtttgcatac ccatcgagaa
     7021 gctgaatcag cactttgtca ttgccaaaat ctagcccagc agcgacggac agctcgcgca
     7081 gcgcttcgct ttcttttttg tcttgcagat acagcgcggc cagactttcg tagggctcac
     7141 ttctatctgg atcgagcttt ttcgcgcgtt catattccgc catcgcagcg ggaaatttct
     7201 tttctgcggt atacagatcg cccagtcgct gccgcacatc gaagccgtct cctccgatgg
     7261 aaatcagctc ccgaaacagt ttttctgctt cactgcggcg tcccagtttt tgttgcagct
     7321 cggcatcggc cagcactgct tctttgcagc gtggatcgag cttgcgcgca accgcgatct
     7381 gtccggcggt ttctgcgggg tcggcaccac ttcgcgccat cgcgacggca agcagtcctt
     7441 ggagctgcgc attttgcgga ctttggcgga ttttctggcg cagtccttca ccatctgcgt
     7501 aatcggaagg gcggatgaaa aacgttccat cgtaggcggc gagctgcttg cgcagatcgg
     7561 aacggaattc ctcatccagc tttgcaatcg gcattcccga tatctgctgc agcacgtctt
     7621 tggtccgttt gccggcgcca aacagcttca gtgcttcgac gatcttggga aaaccaaagc
     7681 gcttggccaa aaaagcgacc gacagcgcgg cttggtggta cgcaacgacg acgtggctct
     7741 gcgaacgggc gcgcgtaaaa ccgatgttca gatccgccat cgaaaggagt ctgtcatcac
     7801 gcagcgctgc tgcgacttcc gcgtgggttc gccggctcca cgatggctgg atctgcatgg
     7861 tttcccattc cgacagacct tcagtaaacc agcgtggcac ttttccgttc gacagttcga
     7921 tcgcaaaaac gtgtcccagt tcatgccaga ccataagccc ccaaggcagc ttgccctgcg
     7981 acggtgagcg acctgtgacg acccggctaa acgtcacccc gagcatgcca gggtccgcag
     8041 aaggaagccc aaatgtccgc accgaaaagt tgtctggttc tttgtacagc tcgatcgaaa
     8101 tcggaagctt tggggaaaag ccgtagcgac gggaaaactc cacatattct gcttgcacga
     8161 gcggctttag gataggaatc agcagttcct cttcgtcttt gtgaacgcga atccgcagac
     8221 cccctttgcc gggtttttgc ggatcgatgt cgacggtcag aaccttgtac gttttctgat
     8281 ggtacttttc gaaaagctcc agcaggttaa acgtcttgcg attgtagcgg tctcccttcc
     8341 acgctttcag gagtgtttga tagctccgct catcgtcgcc aagctgcaaa taactgcgtc
     8401 ccagatcccc cagcgcataa tagtctttgg gctgtaactg caccgcttct tcgagcaccg
     8461 tcacttgcag ttcatagcgg tgctgaattt gcagtcggtc cgacacgatg cggtgaaact
     8521 gactaaacag cggcgagcgg cgcagcgtct cggctttttc tgcttcgtat tctttctgtc
     8581 gatcgagcag gagcagtgcg gcggccctta gcgcacgcgc gggcagatcg tagccattgc
     8641 gggcaattgc cgggtccaag attgcaagcg catcttcgta ctgttcattg tcgatcagaa
     8701 tttccgcttt gatcgcgagc gcagccggat ggatcggatt ggttttcagt gtctcatcga
     8761 taagctcgct ggcctcggtg acgtcgttgc cttgttcgag cttgatccgc gccatcagcg
     8821 ctttggcatc cggattctgg gaatcgcggc ggagcgcgtc gtgggtcgat tcctctgcat
     8881 gtccgacttc gtacttttcg atccgcaggg tactcagcgc cagcgacgcc agaacgaact
     8941 cctcggtcag cttcttttct tttgccagcg ccaccgcatt tgtaagctgt tcgtaagcat
     9001 cctgatagac ccccaggtag tgcgccgcaa ggcccagcag acgcacggat cggggatctt
     9061 gcaagttgag cttgtcagcg tcgtgatcgt cgaagaagcc attccagatc gcgcgctctt
     9121 tgtcgcgctg tccgctctgc cgatacgctt gtcccagcgc cagccgactt gcgagtgcct
     9181 gcggatgatc ttttacaaac ggttcaagtt tttgaatcgc ctgcgggacg tcaccttgca
     9241 gacacaggct ctgcgcgaaa agcgcttcca gatcgtcgcg gacttttgcg tcttttgtcg
     9301 tcgggcgcag cttttccgcc agctgttgcg cgtcgcttgg cttgccgctg cgcagcgcgt
     9361 gttgaatctg ggcaagtggg ccatcaaaac ggaacgcggg ctgcacaggt tcggtgtctt
     9421 tggccgtcgg actgggcttt tgctgcatgg gtttctgcgg tggactggcc aagcccagcg
     9481 acgacgaaac gacaagcggc aacacaagac cgagcatccg agcagcacgc gacagcatct
     9541 tggccagtct acacaaggaa agcgcctgtg cgctcgtcta tcggcgagcc aggcgatggt
     9601 agatcgtgtg cggcggaacc cacgcaatca gcctacgcaa gggcagggcg aagtgtcgtg
     9661 caaaggcgat gctgatgcac agcgcgctca cggtgtaggc tgctaccgat gcccacgcgg
     9721 cgccgatgat tccgtaccgc ggcagaagcc acaggttgag cgcgacgttt gtcacaagcg
     9781 ccagcgtctg cgccaaaatc acactcggcg gatagccgcg cgctcccgac agcgccccag
     9841 cgagcggtcc cgaaaaacac agcgccgagc agcccaacag caagagcaga cacggcaaaa
     9901 acgatccggc aaagcggtcg ccataaaaca gcagcagcag cggttttccg atgaaaaaca
     9961 tcgcaacgca gctgcccaga cccagataaa gtcccagctt ggcggaaagc agcgcctgtt
    10021 ccccgatgtc ttctttggct gccgctttgg caaacaagac cgcattgagc gtacccggca
    10081 gcatcagaat cagctcggca acgcgcgcgc cggcttgata aagaccgagc tgatgccgca
    10141 tcgccagcgg cggcaaaaac agttcaatca gatacacatc gatccgcagc agcagcatgt
    10201 ccggcaagat gtggaagaag ttgcggagac ttgtcccata cagtcgtttt gtcgacggga
    10261 catcggaatg ttcgggagtc tttgggaaag acactgcttt gttgtgggaa tcgaaaaaca
    10321 cgcgcaatag gaatgccaaa cagagtcccc acaggctcag cgcttgcagt ccgagcgcgg
    10381 tttcgagcgc gagttttttc cacagaaaca gacccgccac cgacgcaagc tgaaagacag
    10441 agagcgccaa gctgagccga ttttgggagg caaagcgctg gctcccccac agcagcgcat
    10501 tgtgaatgtc gcgaccgagc tgcccgacca cacagaggat gaggagcgcc gtgacaagcg
    10561 gcgttcgatc ggtcaggaat ctgaaaaact gtgacggcag aagtcttgca agcgcgattc
    10621 ccacaagcga agccaaacag agagccaaaa gcacatggcg gattcctttc caccacagat
    10681 cacgcaccgc gcgcggctgg ccttgcgatt ctccggtcaa caccgccagt ccgcccggca
    10741 gtcccagccc gagcagcgtc gtcacaatgt ttacgtcgac cagcaccgcg ctatagcgcc
    10801 caagtccgtc cggtcccagc gcccgcgccg tcagtgcacc ctggataagt cccagcggca
    10861 ggatcgcaaa gcgtgtcgcc agcgtcgata caaccgagcg cagaagcgaa ggcgttgacg
    10921 ccggctgtgg catgtcgcgg gtcaaaccat ctgtcctttg gggtacttta cttcaccatc
    10981 tgggcaggtg gggaagcgga agtgcggtgg gcctattcgc gttcgttgtg aacgtcggtc
    11041 gacaaaagcg ccagcagcgc atctttggtg tcgaacttgc catgcagaag ctgatacagc
    11101 gccgacacaa tcgaggtccg cacatggttg cgatgtccca atagatgcgc ttctcgcacc
    11161 atctgcgggc tgtcacagac ccgtcccact tgacgcaggg cttcggcaag cggaatgcgc
    11221 tggcccaaca gtcgacccag ctcgaaatcg gcagacccgc ggcgttccgt cgctgcgacg
    11281 aactctccga ccccggccag accgaaaaaa gttcgttcat cggcaccgag ctggacggca
    11341 atccgcgcca tttcggcagc acctcgggtg atcaaaatcg cgcgggccgg ggttcccaaa
    11401 tccaggatgt tggcgacacc cgcggcgagt gcaatcaccg acgatcctgc gcgtgccagt
    11461 tcgacaccga tgagatcgcg cgtggtgtac accaaaagcg agtgacccga cagcacctga
    11521 atcgcttgcg cgccgacttc atcaaagcgg ctgccgcaga cgagcgccgc cggggcaaac
    11581 tcttccaaat cgggggccag cgccgggcca gccaagacgc cgatcttgcg aatcggtgtc
    11641 tcttcttcga tcgcgcgtga aataagctgc gactgtggac actcgtcgct cgcgggaatg
    11701 acgctgccaa tcgcgtggat catgctctgg ttgccgtgca gatgcggcgc agcgagtcgc
    11761 agcactttgc gcaccgaagc ggcaggcacg gccatgaaga tcacatcggc agccgctaga
    11821 tccgacagaa caatcgacgt aacctgggcg cgctcggcgc tcggtgcaga gtgcacagca
    11881 tgaacatcgg gcacttcacc tgcggcggca ggcacgtcgg tgtgatgtac gctggcttcc
    11941 tgttctgctc ggctgtcttc ctgtccggcg cgaatcagct cacgcggggg gcggtagtgc
    12001 agcactcgcg caattcgttc gggcgaacga cgctggttgt gcagcagaaa agacgcgagc
    12061 gcgcgggccc agcgaccgcc gccgatgacc gcaacagaaa ggcttgtcat agctcatccc
    12121 ccaacgattc gattcggttc tggtagtagt acagaagctg tctgtctccg acggtgacgg
    12181 cctgcgaagg cgtgcgaatc gccgccggtt ctgtgtggta actggcaaac gcagtgagcg
    12241 ctgcatcgac cacttgttcg gcgctgccat cggcgcgtgg gcccaagtgg tgtttgcgat
    12301 tgcgcagatg attttgcagc cgctccatgt ccgccaaaag ttcctgtgca gggacatgca
    12361 gatccagcgg ctggcgcagt cgacgataaa catcgagtcc gggcagcgcc acgcagaccc
    12421 gtcgccacag cacatacgca agcaggtggg tcggcaaaag cacggtttct cgacgatatg
    12481 cacgcgaaat cgccaggccg gtctggttca tgtattcggc gtcgcgctgg ggcacatgcg
    12541 ccggctttcc accggaccag aaatagcgcg ccagatcgac gaccatgcca tgcggcccaa
    12601 gcgattcgcc gctgtccgta atcggattgc cgagcacgtc catcggttcg ccaaagcgaa
    12661 tcacaatcgc gcccgtgtgc gtcatcgtcg aacgcacgaa atcgaccact ttgcgccact
    12721 gtgccgattc gtcgtcttcg atgatgaatc gggctttacc ggtttctttc aaatagtcgg
    12781 caatcagcgt ttcggcttcg agcacaagct gctgattgat ggtgacgggc acgatgaaaa
    12841 tcggacgcac cggaacgccc gccacaagac cggcttgccg acggaaacct tcgacggttg
    12901 cggtcagcgc ggtgcccaaa agtcccagct tgagcttgtt ttcgacgcgg cccgatcgtg
    12961 aacgggtgcc acccggaaaa aacatcgagt gatagccgcg ctccaagatg acctgcgaat
    13021 aggtcttgag cacgtctttg tacagctcat ggcgaatgcg gcgatcgacg cgataggcac
    13081 cgcagttgcg catgaaaaaa cccaacatcg gattggtaaa caggtttttt cccgcgccat
    13141 atgtcgtcgg tggcagcccc gccgcttgca gcgaatagcc catcaccggc gaatccagat
    13201 tcgacaggtg cgtcggtacg cagaccatcg tgccgtgcgg cgcgagcgct cgcagcttgt
    13261 cgagcgggcc ttgtagctgt agccgttcgc caagctgctg gagcagcgcc gtcggctgca
    13321 gcatatgaag cggcagcttg agcggacttt ccggcgacag gagcagcgac agcgcgggcg
    13381 gcacgacctg cgaagcaaag gtatagacct gcttattgaa gcctcccgcg acatcgttgg
    13441 cgtagtcggc aatcaaccgt tccaggtcat gttccagcgt cgtgcgtgac ttgccgccaa
    13501 tcgatcgcca cagccgcttc cattccgacg ggctgcgcac ttcttcttgc gcggggcttt
    13561 cggacagccg acggatttcc tgcaagcagg catcgtttag cacgtattcg ggcgggctcg
    13621 tgagaagcac cgtctgaagc gctcgccgcg tcgtttcttt tacgatgttg tcgcgctctt
    13681 tgtgaaagcg cagaagtgga tagtcgcgaa ctggctggaa tcctgacacg ggaaccaatt
    13741 gtaacatcgg gcgtaggtcg tttggggcgg cgttttgtcg gctttgttgt ctgaatgtca
    13801 cgagattgcc gcgaaaacac cgcaaccctg cctagccgct gccgacaatc ttcgatacca
    13861 agccgagttg gaaaggatgc cggcgatgct ccactctcat ctgaaaaacg gtctgcacga
    13921 tatcttccat cacttcgact cgaaccactc gacgcatcca gcgccgcatc atccggcgcc
    13981 gcatccagcc ccacatcatc cggcaccgca tccagcgccg catccagccc cgcatcatcc
    14041 ggcgccgcat ccagcgccac atcatccggc cccacatcca gccccgcatc atccggcccc
    14101 acatccgtcg ccggtttcac actggacgga tcatgggcac gttggcgacc catcgggaat
    14161 cttggatcac atccacgtca ctggcgctgg ttcgcggacg gctcgggccg aaggtctgcg
    14221 ccatggcggt gtggcagcgt cgcagaccat cgcggcgcgc gatacccatc gggtgttgga
    14281 tcagcacaac ctccgtcacg aatacgaatc ggtgggtcag gaacatcaca ttccgccagc
    14341 gctgctcgcc gcgattgcca gtcgagaaac ccatggtggt tcggcgcttg cgcgcaacgg
    14401 caccggggat catggccacg gctacggcgt gatgcaggtc gacaaccgtc acggacagca
    14461 tcccgatcgc agccaaggcc cgtatggaca cgcgcaccta gagcaagcgg caggaatttt
    14521 tgatcacaag ctggccgcgg tacgacatca gttcccacac atgtcgcctg aagaccagat
    14581 gcgcacagcg gtatctcgct ataacggcgg tcgcggactg cctggctcgc gttcggatgt
    14641 cggaaccacc ggcggagact acggcaacga caccatcgcg cgtgcccaat attacgcgca
    14701 ccactgggcg gaatagtccc ccgcgagatt cgttttctcg tcggaaatcc agcctttcgt
    14761 tatgtcttcc gcagcagaat caggttcatc acaccgctgt ttaacaccaa g
//
LOCUS       DAORGN010000127        14588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1702_length_14588_cov_8.123994, whole genome shotgun sequence.
ACCESSION   DAORGN010000127 DAORGN010000000
VERSION     DAORGN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_14588_cov_8.123994"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKL17_13950"
     CDS             complement(<1..615)
                     /locus_tag="PKL17_13950"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK45887.1"
                     /translation="MKQSSFAKAVRAQIAARQEAERCLIPTQRGSVLRIPLIYPSPYR
                     VAMSSLGYQVVFRLLHDPKRPQVSAERAFLPDDVDGHKQARLPLLTYESGSRVNESPV
                     ILFSVAYELEITGLFACLELADIPVLRSERTTAHPLVICGGPLTFSNPLPLWAFADVV
                     VMGEAENVLPVVVETLLQSGLGDAPHDEAQRRAFLRTLGQLPGVW"
     gene            complement(640..873)
                     /locus_tag="PKL17_13955"
     CDS             complement(640..873)
                     /locus_tag="PKL17_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45888.1"
                     /translation="MRKIAILFAFGLLSSSLSGCANIGSAMGGNTSMTGEAWYVKTTG
                     MGGLIFSSKVFYCPQPNTAGPAQCKEAKMVEGK"
     gene            complement(886..1083)
                     /locus_tag="PKL17_13960"
     CDS             complement(886..1083)
                     /locus_tag="PKL17_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45889.1"
                     /translation="MRSLFLLCALSLSSVGCIHVTAVPSVQGKAFVVKTTPFGGSFWN
                     CDATSGEPTCYKVQEIPASAK"
     gene            1448..1666
                     /locus_tag="PKL17_13965"
     CDS             1448..1666
                     /locus_tag="PKL17_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNK45890.1"
                     /translation="MAKGNVKWFNDAKGYGFISVSNPNGQDVQDVFVHHSAILADGYR
                     TLAEGQEVEFDILQGEKGLQAANVRRAS"
     gene            1790..2872
                     /locus_tag="PKL17_13970"
     CDS             1790..2872
                     /locus_tag="PKL17_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45891.1"
                     /translation="MSRGPLLHIRDLSIGFRTGPEQANRYLLAVDRVSLRVPHRTSVG
                     IVGESGSGKSVTALSILRLLPEPPSKICGGSILYRGVNASTPDGTTATTDSEPLDLTQ
                     LDQKSLQKLRGNRIAMVFQEPMTALNPVYPIGDQVGEALRTHRQLSRKQSLSAAADLL
                     DTVGIPDAQRRARDYPHQLSGGQRQRVMIAMALSCQPDLLIADEPTTALDVTTQAQIL
                     DLLARLQAELGMALLLISHDLGVVSEVCSVVAVMYAGQIVEQAGGHELFRTPRHPYTA
                     GLLSAVPRLHGGVERLREIPGRVPRLDEMPSGCRFADRCSRVTSLCRQQAPELRSVDN
                     RPFDLPLAHLVRCHHPIDEHGSGACA"
     gene            2869..3924
                     /locus_tag="PKL17_13975"
     CDS             2869..3924
                     /locus_tag="PKL17_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK45892.1"
                     /translation="MSNLVEIEHLCKHYPLGRRLFHKSDVVRAVEDVSLNIARGQNLG
                     LVGESGCGKSTLGRTVLRLADPTSGKVTIDGVNVTALSAKELTAFRRRAQMIFQDPYG
                     SLNPRMTVRMTLSEPLRVHGLCASASIEKQRVAELAEQVGLSEQSLDRYPHEFSGGQR
                     QRIVIARALAVDPLLVVADEPVSALDVSIQAQIVNLLRDVQTKKGLTYLFISHDLKVV
                     QHLCSEVAVMYLGRLVERAKTETLFRKPLHPYTQALIAAIPRPPDAPSLPDASGDGVT
                     LGYRSRPLLLGGDPPSLMSPPSGCAFHPRCRRYEEAGRPAICRQERPKLVTISVGKPG
                     DLPSQVACHLQNQTKPE"
     gene            3934..4416
                     /locus_tag="PKL17_13980"
     CDS             3934..4416
                     /locus_tag="PKL17_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45893.1"
                     /translation="MAQTEINSAAADSFDDFLQAAIKDYADRGWQRPNFVALLIASGQ
                     SASLAKNALSTGDGLKTVALGTVGVMALRVILTRVLAGPIGLILTGVSVAGLVAFFVR
                     HQREIVSKTARFRELIERTKASFDESQTGYRQNRMDARERNLMIDGLLKRFLRECDEI
                     "
     gene            complement(4413..5078)
                     /gene="modA"
                     /locus_tag="PKL17_13985"
     CDS             complement(4413..5078)
                     /gene="modA"
                     /locus_tag="PKL17_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNK45894.1"
                     /translation="MAAASDVEPVFGALCESYKQKTGRDVVLSFASSGALAKQIEHGA
                     PFDLFVSASEEHIARLRKKGRLVPSTIRSYARGQLALWTAGDGQLPGSLAELVAAPNL
                     RIAVANPEHAPYGSAAVSALRSLGLYEKVRARLVFAENVRQAQLFTESGNTDVSIIAR
                     SLAGRSGRFVEVPLVLHPPINQTLAVIKGGDETHAAELAELLDTPQGRRLLRDSGFLP
                     PTD"
     gene            5303..5734
                     /locus_tag="PKL17_13990"
     CDS             5303..5734
                     /locus_tag="PKL17_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45895.1"
                     /translation="MHGDSEASVEKTVSESSGRSRSAPIERDDPDTGSGLDGRQIFFL
                     LIGSAVFACLVFAIGVLVGRRMERNAAAQAAQQSATDPLSILDEIATAEEALTFHRAL
                     APPSPVPSPRPNRPTAAAAPAPAASAEVPARPAAAPTNQAH"
     gene            complement(5739..7481)
                     /locus_tag="PKL17_13995"
     CDS             complement(5739..7481)
                     /locus_tag="PKL17_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNK45896.1"
                     /translation="MAKGRYPTLHVSGGSLMSLGLILLVLGVAVGKWSIAALGFASLS
                     LLCASYVTFAWRVSLLWRRHLELLWWLPRAATSEGLVAGRPVEVQLTLRNLSPLRMRV
                     TELRIFCSRCIHPQTSPPPIDLPAHSEATTQFTLFVAQAGEWFVHGATVRLCDTLGLF
                     SLEAYFPSALRLKVMPPKLPKVVFDVSRTATGVSEQRVGVHSLRQRGLGGDLRELREY
                     VPGDPFKLIAWKATARSPNGRPLVKELERETLRVHYLILDIGVTMREGMPGQWKLDHA
                     LQLCQSYSRSVLEGNDRLGYVSFDGEVYQHIKAGDGPVLRRRLSEKLLEVMHVVEPSF
                     VAMTDGELFAAVARYLRQQEAIEARLPGPPPPDDAAAWAQVAVAPTGERISRTQVVQA
                     VRRLLRSEAPIVTSAQTDDQLLRQFCRERGIELPYGQRSSTERARGLAQALELAAAMP
                     GAQVVLISDLLGLSLDEPRLRQAAALCRRRGLGLLCVRPEARAYLPKTLLHDEEGARA
                     ATIFSWENDRAEAAAHRELSKLGFHVVPTGPEEGFAQILSRKTGRGGQSSQARDTEQR
                     QQGNQLVASLLVRS"
     gene            complement(7487..8494)
                     /locus_tag="PKL17_14000"
     CDS             complement(7487..8494)
                     /locus_tag="PKL17_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK45897.1"
                     /translation="MATETNPIHPQSLMQAAELCQAVVEEVGKVFIGNPGLTQGLLIG
                     LLSRSHILLEGVPGVAKTTLAKGFAQVLGCDFKRIQFTPDLLPSDITGSYILDQSKGT
                     FELREGPIFSQIILGDEINRAPPKTQSALLEAMAEGQVTLEGQTRPLAQPFMVVATQN
                     PVEHEGTYALPEAQLDRFLMKLYVSYPEPAAEKQMLRTYSQPRPTVRTVLSPQAIVEL
                     QAMSARVHLDDEVLEYVLDLIHFTRKHRRVAIGASPRASLALLHAARARALLLGRDYV
                     LPDDVKALAAPVISHRLALIPEAEMEAVSPVSVVQEALGQVAVRPSEQRRSKPQVERG
                     R"
     gene            complement(8506..9840)
                     /locus_tag="PKL17_14005"
     CDS             complement(8506..9840)
                     /locus_tag="PKL17_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45898.1"
                     /translation="MTVLSTLALLAALATGSATDGVSLGRDFHVGHKGWNGLSELKRL
                     ADDLGCPLQVRRTLDWSSLDGQDVLIVLHPETELWDSATTAYLAAGGRMVIADDFGKS
                     EKVLDSVGIHRKTASWPAGVRFYRPDQSLPVALPVRGTSLGKATSELLGNHSSYFSSS
                     LPATYAFAPGASLVIEANVGHGRLVAIADASVFINNMLELPGNREFAAHLIVDLCRLQ
                     QDHLVLLYGPFAQFGTPPAALLGAPTSDSGHDVPDKLNRALGGANLNIQQTIKGRGKI
                     DDPDVVTLLGLLFCVGSLLLFLRYLPLPGQGQDAGFAQPARPPDTGLFASVQRYAGGA
                     GQVTWGYIYPATLIREETLLRLARYLPSNLSSDGIPMEPKQVRDALRDKASPRAAELG
                     YKLWHGLRGLRDGASASSPAQLLRTQISERKMAEWYKLATELFAELDRVEKV"
     gene            complement(9837..10031)
                     /locus_tag="PKL17_14010"
     CDS             complement(9837..10031)
                     /locus_tag="PKL17_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45899.1"
                     /translation="MRKLPFFQILCALLLISSLVFFWQSIGHLARRDYVAAGMLTVIG
                     IATLWVGSELARLSLVERDS"
     gene            complement(10051..10827)
                     /locus_tag="PKL17_14015"
     CDS             complement(10051..10827)
                     /locus_tag="PKL17_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45900.1"
                     /translation="MPLAPTVLGINLWLLVQVVPLLLAQSPPSRFLFALVPLPPLLLL
                     LGLRLRARLLAQILLLVGFPLLLTSTDALGSMPMERLNPALAQLLKLGLFLLYVASVC
                     NLLATQASSPGPQGTPAASWDVRAIGGAMPPSPKLRRRAATHYILLALCVALPSLFFY
                     AICLHPAHVRQLLRWLGNPAKVAALQATLLAASAMLWSTLFYFGVMRPLKLHISHDGS
                     LRLSLSQIRQVARRGRPRPHFYVAMTVALVGMAALLFFSW"
     gene            complement(10909..12381)
                     /locus_tag="PKL17_14020"
     CDS             complement(10909..12381)
                     /locus_tag="PKL17_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45901.1"
                     /translation="MAKSRRSPPVSAPSAPPPADSDEAIAKSRNPQSQPPQPRDIVEQ
                     ADDGYVPIGDRVARASLGDARAEDPLGGAFWTLQFAQAVSLIADGGLLWALVFWLSTD
                     GKSSGLPLLSVVSPAIHLSLCVLPFFGLLGDRLPRKLLLVGSLGLRAIVQLALALLLV
                     TKQLGPSALSVCQIVSLGGTLLFEVTCPAMVPTLVTYPQAPRALGYGLSLPRAGFFLT
                     SVFCLLMVAVIGPIAMLLCGFGFLMLALALLRSLTISGQPASHFSVAAAPRQFFDGVR
                     LLLTTKGATWLAALALLSNFIVYPLFALSPYQRLHPTTPTPTSQAIGLPFLSKLLPSA
                     PPPQEQHDLEFWLVVGVVIGAALLQRRLKEVSTDRIFAASLLWFSLGIFLLAVSTSDV
                     VAVLATILLGIALLPLAALTAGVTLLAVADGFRARVAALLLMLFLAGGELGQIVLRPL
                     FVQHGARPVMLGIAVGLLLGGTALSTAQTLPNWLRSPERS"
     gene            12498..13742
                     /locus_tag="PKL17_14025"
     CDS             12498..13742
                     /locus_tag="PKL17_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK45902.1"
                     /translation="MDSRKRTSRRVCGLPAGLGFLAVMVGMSVGEAAPPAKKGESHGE
                     AAPRDVVPGEKVRIALLELGSQRMAAGRFDDAIKVFTDAAAQFPTDPKPLYLRGECQR
                     KKGRLKEAEDDLRAALKLDPSGKDEQMVTVMAELGAVLSDAGRSAEAIAILDQAVKLR
                     PTMFEAFYNLGVAHETLKHWPEAIAAYGKAIKLSPQDSNPKASQADAQYNLAVVYRRA
                     GRLEEAVAPAREAVQLAPDRPHAHLNLGMLLSDTKRLDEAVGELLAAAQLADEQYRNT
                     KNPEEREDFRQVLHRAYWRLGVVQTRREQASDAVVALEKAKALQSSPEVLTDLGLARR
                     KLGEIAKAEAEFRAALMQNPKLYSARLHLASTLAGTGRCDDAARELALLPVDPQFTET
                     TNRIKQRCDYDRQMKTRGQVPK"
     gene            14133..>14588
                     /locus_tag="PKL17_14030"
     rRNA            14133..>14588
                     /locus_tag="PKL17_14030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3106 a         4466 c         4428 g         2588 t
ORIGIN      
        1 ccaaacaccg ggcagttggc caagcgtccg caaaaacgcc cgccgctgcg cttcatcatg
       61 tggcgcatcg ccgaggcccg actgaagcag ggtttccacg acgacgggga gcacattttc
      121 cgcttctccc atgaccacca cgtcagcaaa cgcccacagc ggcagtggat tggaaaacgt
      181 cagcgggccg ccgcaaatca ccagcggatg agccgtcgtc cgttcgctgc gcaagaccgg
      241 aatgtcggcc agttccaggc aagcaaacag gccggtgatc tcgagctcgt aggcaacgga
      301 aaatagaatg actggcgatt cgttgacgcg gctgcctgac tcataggtca aaagcggcag
      361 ccgggcttgt ttgtgtccgt cgacatcatc gggcaaaaaa gcgcgctctg ccgatacctg
      421 cgggcgcttt ggatcgtgca gcagccggaa caccacttga taaccgagcg acgacatcgc
      481 gacgcgatac ggactcgggt aaatcagtgg aatgcggagc acgctgccgc gctgcgtcgg
      541 tatcagacag cgttcggctt cttgccgagc cgcgatttgg gcacgaacgg cctttgcaaa
      601 ggaagactgc ttcacggggg atcggggctg ggcggtgggc tacttgccct cgaccatttt
      661 ggcttctttg cactgcgctg ggccggcggt gttcggctgc ggacagtaga agactttgct
      721 agaaaagatg agaccgccca tgccggtggt cttgacgtac caagcctcgc cggtcatcga
      781 ggtgttgccg cccatcgctg aaccaatgtt ggcgcagccc gagagcgacg acgaaagaag
      841 accgaatgca aacaggatgg cgattttgcg catcttgggt gctccttact tggccgaggc
      901 cgggatctct tggaccttgt aacaggtcgg ttcgcccgag gttgcatcac agttccagaa
      961 cgaaccgcca aatggcgtcg ttttgaccac gaaggctttg ccctgcaccg acggaactgc
     1021 ggtgacatgg atacagccca ccgaagacaa gctgagcgca cacagcaaaa agagggaacg
     1081 cattgactac ctccacgagt gggtgatttc agcgacgttc cgtcggaaat cacacgatga
     1141 gaatagggaa cggtagtgta cggatggcgt gcccgtcaag ccccgtggcg agtccgcgcg
     1201 gcaagcgaac cgaagaagtc gcgatttcgt agaaatcgga gaatttcttg actttgaagg
     1261 cgaggcgcgg caagttccgt cgcagatggt cggatggatt gagaagcaaa cgacttcaag
     1321 ccgactctct ggtgttcggc aaacgccgtc gaggatcttt ttgacgcatc cgggggcccg
     1381 aagtctgggg ttttgccgtc gcggcttttc aatggagcca gcgatcgggg cgtcgcagga
     1441 gtagcaaatg gccaagggca acgtgaagtg gtttaacgac gcgaagggat acggcttcat
     1501 ctcggtgagc aatcccaacg gacaggatgt gcaggacgtg tttgttcatc actcggcgat
     1561 tctcgccgac ggttatcgga ccctcgcgga agggcaggaa gtcgagttcg acatcctcca
     1621 gggcgaaaaa ggcctgcagg ccgccaacgt ccgtcgcgcg tcgtaagcgc gcttcttccc
     1681 acgcctcacg gcagactttc ttttctgctg tgctctttcg ccactcgctg gcgcaaaaat
     1741 ccccgaaaaa ttgaccaacg ctcgtcgctg cccgcatgct cgggcgcaca tgagccgagg
     1801 accgctactt cacatccgcg atctttcgat cggctttcgc accggaccgg aacaagccaa
     1861 ccggtatctg cttgccgtcg accgagtgag cctgcgggtg ccacatcgaa cctcggtcgg
     1921 catcgtcggc gaatcaggtt cgggcaaatc ggtgacggca ctgtcgattt tgcggctttt
     1981 gccggagcca ccgtcgaaga tttgcggtgg ctcgattctg taccgcggtg tgaatgcctc
     2041 gacgcccgac gggacgacgg ccacgacaga cagtgaaccg ctggacctta cccagctcga
     2101 tcagaaatcg ctgcaaaagc tgcgcggcaa tcggatcgcg atggtgtttc aggaaccgat
     2161 gacggcgcta aatccggtct atccaatagg cgaccaagtc ggcgaagcgc tgcgcacaca
     2221 tcgtcagctg tcccgcaaac agagcctgtc ggcagcggcg gacttgctcg atacggtggg
     2281 gattcccgat gcgcaaaggc gggcccgtga ctatccgcac caactgtcag gcgggcagcg
     2341 gcagcgggtg atgattgcga tggcgctgtc gtgtcagccg gacttgctga ttgcggacga
     2401 accgacgacg gcgctcgatg tgacgacgca ggcgcaaatc ctcgatttgc tcgctcggct
     2461 tcaggcggaa ctggggatgg cgctgctgct gataagtcac gacctcggcg tggtgtccga
     2521 agtctgttcc gtcgtcgcgg tgatgtatgc cggccagatc gtcgaacagg ctggcgggca
     2581 cgagctgttt cggacgccgc gacatccgta caccgcgggt cttttgtcgg cggtgccgcg
     2641 gctgcacggg ggtgtagaga ggctgcgcga gattccgggc cgggtgccgc gactcgatga
     2701 aatgccgtcg ggttgtcgtt ttgccgatcg ctgctcgcgg gtcacatcgc tgtgccgaca
     2761 acaggcgccc gagctgcgca gcgtcgacaa taggccgttt gatctgcccc ttgcgcattt
     2821 ggtgcgctgt caccatccga tcgacgagca cggcagcggg gcttgcgcat gagcaatctg
     2881 gtcgaaatcg agcatctgtg taagcactat ccgctgggac ggcggctgtt tcacaagtcc
     2941 gatgtggtgc gtgccgtcga agacgtatcg ctgaacattg cgcgcgggca aaaccttggg
     3001 ctcgtcggtg aatcgggctg cggcaagtca acactcgggc gcacagtgct gcgtttggcg
     3061 gatccgacgt cgggcaaagt gaccattgac ggcgtgaacg tcaccgcgct ttctgcgaaa
     3121 gaactgaccg cgtttcgtcg acgggcgcag atgatctttc aagatccgta tggctcgctc
     3181 aatccgcgga tgacggtgcg aatgacgctc agtgagccgc tgcgagtcca cggcttgtgc
     3241 gcaagtgcga gcatcgaaaa acagcgcgtc gccgagctgg ccgaacaagt tgggctgtct
     3301 gagcagagcc tggaccgcta tccacacgag ttttccggcg gacagcggca gcggatcgtg
     3361 atcgcgcggg cgctggcggt cgatccgctg ttggtggtgg ccgacgagcc ggtcagtgcg
     3421 ctcgatgtgt cgatccaagc gcagatcgtc aatcttttgc gcgatgtgca gacgaaaaaa
     3481 gggctgacgt atctgtttat ttcgcacgac ctcaaagtgg tgcagcacct gtgctcggaa
     3541 gtcgctgtga tgtaccttgg gcgactcgtc gagcgtgcga agacggaaac gctgtttcgc
     3601 aagccgctgc atccttatac gcaggcgctg attgcggcga tcccgcggcc accggatgct
     3661 ccgtctttgc ccgacgcgag tggcgatggg gtgacgctgg gctacaggtc gcgtccgctg
     3721 ctgttgggtg gggatccgcc gtcgctcatg tcgccgccga gcggctgtgc atttcatccc
     3781 cgctgccgtc gctatgaaga agcaggtcgg cccgcgattt gtcggcagga acggcccaag
     3841 ctggtcacga tttccgtcgg aaaacccggg gatttgccgt cgcaggtggc gtgtcatctc
     3901 caaaaccaaa ccaagccaga gtaggcttcc cccatggcgc aaactgaaat caacagcgcg
     3961 gcagcagatt cgtttgatga ttttttgcag gcggcgataa aggactatgc cgaccgaggc
     4021 tggcagcgac ccaatttcgt cgcactgctc atcgcttcgg gacagtcagc gagccttgcc
     4081 aaaaatgcgt tgtcgaccgg ggacggacta aaaaccgtcg cgcttggcac cgtcggagtg
     4141 atggcactgc gggtgatttt gacgcgcgtg ctggcagggc cgatagggct gatattgacg
     4201 ggcgtgtcgg tggcgggtct ggtcgcgttt tttgtgcggc accagcgcga gatcgtcagc
     4261 aaaacggctc ggtttcgcga gctgatcgag cgcaccaagg catcgtttga cgaatcgcag
     4321 accggctatc ggcaaaatcg catggatgcc cgcgagcgca acctgatgat tgatggtctg
     4381 ctcaagcgct ttctgcgcga gtgcgacgag atttagtccg tcgggggcaa aaaaccgctg
     4441 tcgcgcagca ggcgacggcc ttgtggggta tcgagcagct cggctagctc ggcggcgtgc
     4501 gtttcgtcgc cgcctttgat caccgccagc gtttggttga tgggcgggtg aagcacaagc
     4561 ggcacttcga caaaccgacc cgatcgaccg gcgaggctgc gggcaatgat cgacacgtcg
     4621 gtgttgccac tttcggtgaa aagctgcgct tggcgcacgt tttccgcgaa caccagccga
     4681 gcccgcactt tttcgtacag cccaagcgat cgcaaggcgg acaccgcagc cgatccatac
     4741 ggtgcatgtt caggattggc gacggcaatt cgcaaattcg gtgcggccac gagttccgcc
     4801 aagctgcccg gcagttgtcc atctcccgcc gtccacagcg ccagctggcc ccgcgcatag
     4861 gatcgaatgg tcgacggcac gagccgccct tttttgcgca gccgcgcgat gtgttcttcg
     4921 ctggccgaga caaacaaatc aaacggtgcg ccgtgttcga tctgtttggc cagtgcgccc
     4981 gacgaggcaa agctcagcac cacatcgcgt ccggtctttt gtttgtagct ttcacaaagc
     5041 gcgccaaaca ccggctcgac atccgaggcc gcggccactc gcagcggctg gatagtcgtc
     5101 gccgattgtt ttcggcagtg gcaaaggaca agccctagcc caagtcccat cgcttcccga
     5161 cgggatggat ggcgagccac ggtcactttg gggatgtctc cttctggcac atcgacgcga
     5221 agatgacagc gcccagcctg cctgtaaagg gagccgcgga gaatttgcgt ccgtctctgc
     5281 ctcgtgggta actgcacaac gtatgcacgg tgacagcgaa gccagcgtgg aaaaaacggt
     5341 gtccgaatca agcggtcgct cgcgcagcgc gcccatcgaa cgcgacgatc cggatacggg
     5401 cagtgggctc gacggcaggc agattttctt tctgctgatt ggcagtgcgg tgtttgcctg
     5461 cttggtgttt gcgatcggtg tgctggtcgg tcggcgcatg gaacgcaacg ccgctgcgca
     5521 agccgcccag cagtcagcga ccgatccgct ctcgattctg gacgagattg ccaccgccga
     5581 agaagcgctg acattccatc gggcactggc gccaccgtcc cccgtaccat cgcccaggcc
     5641 aaatcgtccg actgcggctg cggcacctgc ccccgctgcg agcgccgaag tcccggcgcg
     5701 acctgcggcg gcaccaacga accaggcgca ctagtcgctt acgatcggac gagcagcgac
     5761 gcgaccagct gattgccttg ctgcctttgt tcggtgtcgc gagcttggct gctttgtccg
     5821 ccgcgtccgg ttttgcgcga cagaatttgt gcaaagcctt cctcgggtcc ggtcggaaca
     5881 acgtggaagc caagcttgct gagctcgcgg tgggcggcgg cttcggcacg atcgttttcc
     5941 cacgaaaaaa tcgtcgccgc gcgcgcgcct tcttcatcgt ggagcagcgt cttgggcaga
     6001 taagctcgcg cttccggacg cacgcacaaa agcccaagac cgcgccgacg gcacagcgcc
     6061 gctgcctgcc gcaagcgcgg ttcgtcgagc gacagcccca gcagatcaga tatcagcaca
     6121 acctgcgcgc ccggcatcgc cgccgcaagc tcgagcgctt gggccaagcc gcgggctcgt
     6181 tccgtcgaag atcgctgacc gtaaggcagc tcgatgcctc gttcgcggca aaactggcgc
     6241 agcagctgat catccgtttg tgccgatgtc acgatgggcg cttcactgcg cagaagccgg
     6301 cgcaccgcct gcaccacctg ggtccgcgag atccgttctc ccgtcggagc gaccgcaact
     6361 tgcgcccatg ccgctgcgtc gtcgggcggt ggcggtcccg gcagtcgtgc ctcgatcgcc
     6421 tcttgctgcc ttagatatcg cgccacggct gcgaacagct caccgtcggt catcgccaca
     6481 aaggacggtt ccacgacgtg catcacttcg agcagttttt cgctcaatcg ccgacgcaac
     6541 accggaccat caccggcctt gatgtgctga tacacctcgc cgtcaaatga cacgtaaccg
     6601 agccgatcgt tgccctccag caccgagcgg gaatagcttt ggcacagctg cagcgcgtga
     6661 tcgagcttcc actgtcccgg catgccctcg cgcatcgtca cgccgatgtc gaggatcagg
     6721 tagtgaaccc gcagcgtttc ccgctccagc tcttttacca aaggccgtcc attgggcgat
     6781 cgcgcggtcg ctttccaggc gatcagcttg aacggatcgc cgggcacata ttctcgcagc
     6841 tcgcgtagat cgccgcccaa accacgctgc cgcagcgagt gcactccgac gcgctgttcg
     6901 gaaacacccg tcgctgttcg cgacacatca aagaccactt tgggaagttt gggcggcatc
     6961 actttcagcc gcagcgccga cggaaaatac gcttccaacg aaaaaagtcc gagcgtatcg
     7021 cacagccgca ccgtcgcgcc gtggacaaac cactctcccg cctgggcgac aaacagggtg
     7081 aactgcgtcg tcgcttcgct gtgcgctggc agatcaatcg gcggcggcga cgtttgggga
     7141 tggatgcagc gcgaacaaaa aatccgcagc tcggtgacgc gcatccgcag cggcgacagg
     7201 ttgcgcaacg tcagctgcac ttccacggga cgtccagcga caagaccttc gcttgttgcg
     7261 gcacgcggca gccaccacaa aagttcgagg tgccgccgcc acaaaagtga cacccgccaa
     7321 gcgaacgtga catagcttgc gcaaagcagc gacagcgacg caaaacccag cgccgcaatc
     7381 gaccatttgc cgaccgccac cccgagtaca agcaggatca ggcccagcga cataagcgag
     7441 ccgccactga cgtgcagcgt gggataacga cccttcgcca tccagcctag cgacctcttt
     7501 caacctgcgg tttgctgcgg cgctgttcgc ttggacgcac cgcgacttgg cccagcgctt
     7561 cctgcacgac gctgaccgga ctgaccgctt ccatttcggc ttcgggaatt agcgccagac
     7621 ggtgcgaaat gaccggtgct gcgagcgctt ttacatcgtc gggcagcaca tagtcgcgac
     7681 cgagcaacag cgcacgggcc cgcgccgcat gcagcagtgc cagcgacgcc cgtggcgaag
     7741 cgccgatcgc cacccgtcga tgtttgcgcg taaagtgaat cagatcgagc acgtattcga
     7801 gcacttcatc atccagatgc acccgcgctg acatcgcctg gagttcgacg atcgcttgtg
     7861 gcgaaagcac ggtgcgcacc gtcggacgcg gctgcgaata ggtccgcagc atttgttttt
     7921 ccgccgccgg ctcgggatag ctcacataca gcttcatcaa aaagcgatcg agctgtgctt
     7981 ccggcagcgc gtaagtgcct tcgtgctcga cgggattttg cgtcgccacg accataaaag
     8041 gctgtgccaa aggtcgcgtc tgcccttcga gcgtcacttg accttccgcc atcgcttcga
     8101 gcagggccga ctgcgttttg ggcggcgcgc gattgatttc gtcgcccaag atgatctgcg
     8161 aaaaaatggg cccttcgcgc agctcaaacg tccctttgct ctgatcaagg atgtacgacc
     8221 cagtaatgtc cgacggcagc agatcgggcg taaactggat gcgcttgaag tcacagccca
     8281 gcacttgggc aaagcctttg gccagcgtcg ttttggcaac gccgggcacg ccctcgagca
     8341 ggatgtgact gcggcttagc aaaccgatga gcagaccttg cgtcaaaccc ggattgccga
     8401 tgaagacctt gccgacttct tcgacgacgg cctggcacag ctcggcagct tgcatcaggc
     8461 tctgcgggtg aatggggttg gtttccgtcg ccacgaggcc tctcctcaca ctttctcgac
     8521 ccgatccagt tcggcaaaca gttccgtcgc cagcttgtac cactcggcca tcttccgttc
     8581 cgaaatctgc gtgcgcagaa gctgtgccgg actgctcgcg gaagccccgt cacgcaaccc
     8641 acgcaagccg tgccacagct tgtagcccag ctctgccgcg cgtggacttg ccttgtcgcg
     8701 cagcgcgtcc cgaacctgct tgggttccat cggaattccg tcggaagaca ggttcgaagg
     8761 cagataccgt gccaaccgca gcagtgtctc ttcgcgaatc agcgtcgccg gatagatgta
     8821 gccccaggtc acttgaccgg cgccaccggc atagcgctgc accgacgcaa aaagtccggt
     8881 atcgggcggt cttgccggct gcgcaaaacc cgcgtcttgg ccctgtcccg gcagcggcag
     8941 atagcgcaaa aacagaagca gcgatccgac gcaaaacagc agaccgagca gcgtcaccac
     9001 gtcgggatca tcgatcttgc cgcgcccttt gatcgtctgc tggatgttca ggttggcacc
     9061 gccgagcgcc cggttcagct tgtcgggtac gtcgtgtccc gaatcactcg tcggcgcccc
     9121 gagaagtgcc gccggcggcg tgccaaactg cgcaaacggt ccatacaaaa gcaccagatg
     9181 gtcttgctgg agccgacaca ggtcgacgat aagatgtgcg gcaaactctc gattgcccgg
     9241 cagctcgagc atgttgttga taaacacgct cgcatcggca atggcgacga gccgcccgtg
     9301 accgacattg gcttcgatca ccagcgaagc gccgggggca aacgcatacg tcgcaggcag
     9361 gctgctcgaa aaatacgacg agtggtttcc cagtagttcg cttgtggctt tcccgagtga
     9421 tgtccctcga acgggcagcg ccaccggcag gctctgatcc ggcctataga aacgcactcc
     9481 cgctggccag ctcgcggttt ttcgatgaat cccgacggaa tctaatactt tttcactttt
     9541 gccaaagtca tcagcgatca ccattcgacc gcccgccgcc agataggccg tcgtcgcgct
     9601 gtcccacagc tcggtttctg gatgcagcac aatcagcacg tcttgcccgt cgagactcga
     9661 ccaatcaagc gtgcgacgca cttgcagcgg acagcccaga tcatcggcca gtcgcttgag
     9721 ttccgacaga ccgttccagc ctttgtgccc gacgtgaaaa tcgcgcccca gcgacacgcc
     9781 atcagtcgcc gaacccgtcg ccaaagccgc cagcagtgcg agcgtggaca aaaccgtcat
     9841 gagtctcgtt ccacaagcga cagccgcgcc agctcgctac ccacccacag cgtcgcaatg
     9901 ccgatcaccg tcagcatccc cgccgccacg taatctcgtc gggccaggtg gccgatgctc
     9961 tgccaaaaaa acaccagact gcttatgaga agcagcgcgc acaggatctg gaaaaacggc
    10021 agctttcgca tcgtcggctc gattccaaac tcaccagctg aaaaagagta gcgccgccat
    10081 cccgacgagc gccactgtca tcgcgacata aaagtgtggc cgcggacgac ctcgccgtgc
    10141 cacctgccga atctgcgaca gagaaagccg cagcgatcca tcgtgactga tgtgcagctt
    10201 caggggtcgc atcacgccaa aataaaacag cgtcgaccac agcatcgccg aagccgcaag
    10261 cagtgtcgct tgtagcgcgg cgactttcgc cggattcccc agccagcgca gcagctgccg
    10321 gacatgggct ggatgcaggc aaattgcata aaaaaacaga ctcggcagcg ccacgcacag
    10381 cgccaaaaga atgtagtgcg tcgcggctcg tcgtcgcagc ttgggcgacg gtggcatggc
    10441 gccgccgatg gcgcgaacgt cccaggacgc agccggcgtg ccttgtggac cgggcgacga
    10501 agcctgagtt gcaagcaagt tgcaaaccga cgctacatac agaaggaaaa gtcccaattt
    10561 caaaagctgc gccagtgccg gattcagccg ttccatcggc atcgacccga gcgcatccgt
    10621 cgatgtcaaa agcagcggaa aaccgacgag caacaggatc tgcgccagca gtcgcgcccg
    10681 cagccgcaaa cccaaaagca gaagcagcgg cggcagcggc acaagtgcaa acaaaaaacg
    10741 cgacggtggg ctctgcgcaa gcagcagcgg caccacttgc acaagcagcc atagattgat
    10801 ccccagcacc gtcggtgcaa gcggcattcg gcgatgatat cacagcgagt cgtcgtgaaa
    10861 attggcgcaa aacgcccaat ctggacccag catcgtgcgc cgcgacagtt aggatcgctc
    10921 gggactgcgc agccagttgg gcagcgtttg cgccgtcgaa agcgccgtcc ccccgagcag
    10981 cagacccacc gcgattccca gcatcacagg tcgggcgccg tgctgcacaa aaagcggcct
    11041 cagtacgatt tggccaagct cacccccggc caaaaacagc atcaacagca gcgctgcgac
    11101 gcgcgcccga aacccatcgg ccaccgcgag cagcgtcact ccagcggtca gcgccgccag
    11161 cggcaaaagg gcgatgccca acaagatcgt ggccagcact gccaccacgt ccgacgtcga
    11221 aaccgcaagc aggaaaatac ccagcgaaaa ccacagcaag ctcgcggcaa agatccgatc
    11281 ggtcgacacc tctttcagcc ttctttgaag aagcgccgca ccgatcacaa cgccgaccac
    11341 cagccaaaac tccaggtcgt gctgttcttg cggcggtggc gcgctgggca aaagcttgct
    11401 caaaaaggga agcccgatcg cttgtgaagt cggcgtaggc gtcgtcggat gcagtcgctg
    11461 gtacggcgac agcgcaaaca gcggatagac gatgaagttg gaaagcagcg ccagcgccgc
    11521 aagccatgtc gcgcccttgg tcgtcagcag cagccgcact ccatcaaaaa actgacgagg
    11581 cgcagcggct accgagaagt ggcttgccgg ctgtccgctg atcgtgaggc ttcgaagcag
    11641 cgccagggcc agcatcagaa agccaaagcc gcacagcagc atcgcgatcg ggccgatgac
    11701 tgccaccatc agcaggcaaa acaccgaagt cagaaaaaag ccggcgcgtg gcagcgacag
    11761 gccgtagccg agcgctcgcg gagcctgcgg atacgtcacc agcgtcggaa ccatggcggg
    11821 acaggtcact tcgaacaaga gcgtcccgcc aagcgacacg atctgacaga cagaaagcgc
    11881 gctgggccca agttgttttg tgacaagcaa aagcgccagc gcaagctgaa cgatcgctcg
    11941 cagccccagc gacccaacga gcagcaattt tcgcggcagc cgatcgccaa gcaggccaaa
    12001 aaacggcagc acacacagcg aaaggtgaat cgccggactt acgaccgaaa gcagcggcag
    12061 ccccgatgat tttccgtccg tggacagcca aaataccagc gcccacaaaa gccctccgtc
    12121 agcgatgagc gacaccgcct gggcaaactg aagcgtccaa aatgccccac caagcggatc
    12181 ttcggcgcgg gcatccccaa gcgacgcgcg cgcgacccga tcgcctatag gaacgtagcc
    12241 gtcgtccgct tgttcgacga tatcccgagg ctgcggcggc tgcgactgcg ggttccggga
    12301 ttttgcgatc gcttcgtccg aatcggcggg cggtggggcg gacggcgcgg aaaccggcgg
    12361 tgatcgtcga gacttggcca tgcagtcgtt cccccaagcg atgcctcgtc agtttatcgc
    12421 cgtcgggccc gcgctgagaa gtccttggcc acgactcgac gttgccggct tcaaaagaac
    12481 agcgctaaag tgcgaacatg gattctcgaa agcgaacgtc gcgacgggtt tgtgggctgc
    12541 cagcgggtct tggctttctg gctgtgatgg tgggcatgtc ggttggcgaa gcggcccccc
    12601 cggcaaaaaa gggcgaaagc catggcgaag cggcccctcg ggacgtggtt ccgggcgaaa
    12661 aggtgcggat tgctctatta gagttgggat cgcagcggat ggccgccggt cgcttcgatg
    12721 atgcgatcaa ggtgtttacc gacgcagccg cgcagtttcc gaccgatccg aagccgcttt
    12781 atctacgcgg cgagtgccag cgcaaaaagg ggcggcttaa ggaggcggaa gacgatctgc
    12841 gagcggcgct caagctcgac ccgtcgggca aagacgaaca aatggtgacg gtgatggccg
    12901 agcttggcgc cgtgctctcg gatgccgggc gatcggcgga agcgatcgcg atcttggacc
    12961 aagctgtgaa gctgcgtccg acgatgttcg aggcgttcta caacctaggc gtcgcgcacg
    13021 agacgctgaa gcactggccc gaagcgatcg ccgcgtatgg caaagcgatc aagctgagcc
    13081 cgcaagattc caatccaaag gccagccaag ccgatgcgca gtacaacttg gctgtcgttt
    13141 accgtcgcgc aggcaggctg gaggaagccg tcgcgccagc tcgtgaagcc gttcagcttg
    13201 cacccgatcg ccctcacgca cacctcaacc tggggatgct gctgtcggat acaaaacgac
    13261 tcgatgaagc cgtcggcgaa cttctggccg cagcccagct cgccgacgag cagtaccgaa
    13321 acaccaaaaa ccctgaagag cgagaggact ttcgccaagt gctgcaccgg gcgtactggc
    13381 gacttggtgt ggtccagacc cgtcgagaac aggcgtcaga tgcggtggtt gcgctggaaa
    13441 aagccaaagc gctgcaatcg tcgcccgaag tgcttaccga tttaggtctg gctcgtcgca
    13501 aactgggtga gatcgccaag gccgaagccg agtttcgcgc cgcgctcatg caaaacccca
    13561 agctctactc ggcgcgcctg cacttggcgt cgacgctggc gggcaccgga cgctgtgatg
    13621 acgccgctcg ggaactggcg ctactgcccg tcgaccccca gttcaccgag acaaccaacc
    13681 gcatcaagca gcgctgtgac tacgatcggc agatgaaaac gcgcggtcag gtgccgaaat
    13741 aagcccgtaa aaaggcgatc ggcaggcaaa agacaggtcg tttcgcaggg ttaggcagtg
    13801 ctgcagcaaa gattttcccg acgggaaaac gtaaattatg aaaataattc tgaaaaaccg
    13861 cttgacccct gcgtgattgc gagtagagtc cttcacctgt cgccgcgatg gcgccggttg
    13921 gttccgctcc tcaaacgagt gaaacctcag cgaaaaaaag cgataaaaaa gcttgacgcg
    13981 ctgaaaaagg ctgatagagt ggcgaccccg cgctgatgag aaatcaaaag cgcaaaagtt
    14041 tggtccttga aaactgaata gcgagaagca gggaaaaata catacatgag taccctgtcg
    14101 aatctttgat tcggcaacgg gttcaaacat ctcaattgga gagtttgatc ctggctcaga
    14161 gcgaacgtta gcggcaggct taacacatgc aagtcgagcg agaaaggggt ttcggcccct
    14221 agtaaagcgg cggacgggtg cgtaacacgt ggataatctg cctcgtggcg ggggacaaca
    14281 gttcgaaagg actgctaata ccgcatgtca catcgaaatc caaggatttc gctggaaaag
    14341 ggggcgcccg caaggggctc tcaccacgag atgagtccgc ggcccatcag cttgttggcg
    14401 gtgtaataga ccaccaaggc taagacgggt agctggtctg agaggatgac cagccacact
    14461 gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat tggacaatgg
    14521 gcgaaagcct gatccagcca tgccgcgtga gtgatgaagg tcttcggatc gtaaagctct
    14581 gtgggaag
//
LOCUS       DAORGN010000128        14568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1707_length_14568_cov_6.002549, whole genome shotgun sequence.
ACCESSION   DAORGN010000128 DAORGN010000000
VERSION     DAORGN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_14568_cov_6.002549"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PKL17_14035"
     CDS             complement(<1..1332)
                     /locus_tag="PKL17_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45903.1"
                     /translation="MSGSEARSSTRVPKKLPWRRAAAVLGLSFIVACGGSGGGGCGAG
                     GLTKIPNGYPVDKRIDNAIQLRVTKGFFDFLETNGKAIVTKLLPGGGDIPVPSQCGGD
                     TEICCGQMCKVKLDYQSLKFDPKPPTTTALTLRAKIKTSEPMKVKAKVKLGPIPVTVD
                     CKLALDTARSGKPDVGVTMAVATNVNATTKLTSFDFDDKSVDILDLDSGDIDISGSFG
                     CDLLDWIKGPFIGTLKDQLKRQLAAPLDSLMCQKCSTKPDCSSLANQGCVNNYCMRDG
                     TCLQLIGTEGRIDLGSALGAYSPAAKTAALDLYAAAGNYATVEALPTAGMSLGMLGGT
                     TSQNRSTCVPVRPAPTGPAPGKSAAFAGGTTPNGKPYHIGAGISTLELNSVGYTLYDT
                     GALCLDVGTAQVEMLSSGLLSALIPSLNDLTRGANSAVKLVGRPQQPPTFVL"
     gene            1537..2607
                     /locus_tag="PKL17_14040"
     CDS             1537..2607
                     /locus_tag="PKL17_14040"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNK45904.1"
                     /translation="MSSALFQRVTRNVLPSLHPTKEQWVGCLIGQCIGDAVGAPVEGL
                     LPKDCKRHAEKVLRHGLLDDPFLGQTRFGQYTDDSQLARELLAGLCEHGELNSADYAA
                     RIVRLFVQGEVVGAGKATAEVAHRLARGVASDEAGIPAPSAGNGSAMRAAPIGLWYAD
                     DPVKLVAAAREQSRITHQDIRCAAGAVAIAGAVAIALRPSPIVVEPFLDELAEWVRCI
                     DETVAFDVNQVASWVSLSPDAAATFMARAGLCPGHDGDGEWRGISGYVVSSVLWSLYS
                     FLRSPEDFVQTIETAVSVGGDVDTTAAMAGAMSGAHLSIDALPMTLCERLTDQGRWGF
                     GELSQLAESAFHTWQAQRRAVS"
     gene            complement(2613..3299)
                     /locus_tag="PKL17_14045"
     CDS             complement(2613..3299)
                     /locus_tag="PKL17_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45905.1"
                     /translation="MAEHPSASEPAPSVSDSAVLAAVDEQTPDKARARILDIARIDSE
                     NAADAAQRERDNAMRAEIVSKSVPERYLQLVQELREAVRLFNQSLRHPDGHPIPTISW
                     VETPNVALGDANSGDGMRVRIARRGATFDLILRFATRAARADVPLIEGHGDYGNPQQG
                     RTKTMLRIEGWVEKGKPIFWYSLDFKRQSVAIDEVAERIVASVAANDYSLLSRDLDGE
                     MLTDEPADSK"
     gene            3414..4955
                     /locus_tag="PKL17_14050"
     CDS             3414..4955
                     /locus_tag="PKL17_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45906.1"
                     /translation="MQKHAPSLGSSGLSSAPFPASQNSDGGSVAIWTDVGIGWLVTSL
                     ALLALKSPMLNDPYFWDELGCYFAQVYQMSTRLSAYLAAKPEYVRSPLLTSLLSLLHH
                     FFGPSRVLQHGAMVLICSLVPPSAYALTRQLGGTKKIAFLAALLCFVTPAFFAQSAMV
                     QMDLPATGICTIAFVCLLRKNLLGFALAGSMAVLIKESTYYVCLPAALVVYGRLVLLD
                     RRKPLALSTILRLWPTGIPGITLFLWLLVHRRLTGAMISGDHTAVIGLHGIFPSLLHN
                     FVEGRRFALSLCALAYLFAMRMPLGSEQKIRVIATAVLWMALPFCFPGHLVRYLLPSL
                     PALCALAALGVSLLPIPRRAGLATLLVFVLVSGFRGDSFHENPPFELEGNLSYRLLLQ
                     EQVAVVRRVAASGSKLVIADFPFANMFPAPREFGYLENWIPTLPLHRMTKPDDLCQGD
                     VVVMTSVSQDKEPLIESALRVGILVPWFVGAGDNFRVGNSLFVPSFARHDLRIRVYRV
                     TCPPK"
     gene            4969..5793
                     /locus_tag="PKL17_14055"
     CDS             4969..5793
                     /locus_tag="PKL17_14055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNK45907.1"
                     /translation="MFDSHCHLHDDRSFALAPDMIARAQQVGLRGLVLAGVDRDTWGR
                     QDALRRQFGSPDFSIGVAYGVHPQLIPSLSPSALEEQLAALHCAARGTLEIDGNLIGK
                     PDAIGELGLDAAADQTQQNMTSQTHAFRQQLALARDLDLPIVLHILKTHAQVLQILKQ
                     DGIPRAGGVVHSYSGSAEQCREYVQLGLCISFAGGVTREHAPKLWASLALVPEDRLLV
                     ETDAPDQTPMAHRPAHNEPAFLPAILAAVAKQRNQTVKQTAILTEQNARRLFRLPR"
     gene            5812..6693
                     /locus_tag="PKL17_14060"
     CDS             5812..6693
                     /locus_tag="PKL17_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNK45908.1"
                     /translation="MDTTLALPGTSLPESDDGETRYRLHRRFDRMGRLVGDEKMERLF
                     ASHVLVIGVGGVGSFAAESLLRSGIGQLTLVDFDRVCVTNTNRQLQAMRGTVGKLKAQ
                     VLAERLALVNPQAKVSVISRFYDSATSELILDRDPRPDFVIDAIDNITAKCHLIAACK
                     QRGLPLVSSMGAAGRCDPTAIRIGDLADTRICPLAQEVRKILRTKFAFPQEGLFGIPS
                     VFSVEKPREPIELHYDDGMGFACVCPGGKNEHHSCEERRVIYGTTSYVTGSFGLFCAS
                     VVVNSLTELRGSKPGSV"
     gene            complement(6706..6915)
                     /locus_tag="PKL17_14065"
     CDS             complement(6706..6915)
                     /locus_tag="PKL17_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45909.1"
                     /translation="MFKVFQTKTLLRIWVMRYCESDRYPTCERYILAATGAPVPRTLL
                     PNGQKLAEGRAASANEKDVMPLRRV"
     gene            complement(6970..7398)
                     /locus_tag="PKL17_14070"
     CDS             complement(6970..7398)
                     /locus_tag="PKL17_14070"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNK45910.1"
                     /translation="MNRDIIGNSVIGLALGFALSCIGFSSMTELHKMLRFSDQRLLYT
                     FFLAVLLLAGSWKLIAKLAKTKPDWAPKPIHRGTIAGGLLFGIGWALCGACPSAVFIQ
                     LGEGQAMALCTLVGIGLGNWMYAILHERVLRFDTGSCQSD"
     gene            complement(7401..8015)
                     /locus_tag="PKL17_14075"
     CDS             complement(7401..8015)
                     /locus_tag="PKL17_14075"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNK45911.1"
                     /translation="MLHNWPFWLGGTTLAAICVAHWLSIGRMLGVSGRYSAIVNRIRF
                     GAVPDDNIDDAKLAQELEAATLAAFGKDALAEVLESHEPPPAAQSVASEQRPPQSLSA
                     HLVFLASLILGGFLAAVLDGRFSVDFSLRDDATARLLGNSRAAQALLLFGGGILVGIG
                     TRMAGGCTSGHGLCGTSRLQPGSIVATAAFFLSGATLTRLLFGG"
     gene            8436..9287
                     /locus_tag="PKL17_14080"
     CDS             8436..9287
                     /locus_tag="PKL17_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNK45912.1"
                     /translation="MPASASEKHVAEVLDRVLQSKRALEASPACKEGCVFLSAWDFDG
                     TILKGDASEGLSEDNHVVYPGLAQKSIEAGFSGLYKKTEFQKFWHDYEEMDQKQGHIP
                     AYTYLPKTMKGTKADEIKKFAGNYYATVLKPYYFESSIDIFQKLKQSGVIPYVISAAP
                     HVFVSSSGPTLGIDEQHIFGIELALTKDGVLTDRVIDPIPYAEGKAKRLAMIVDQEKA
                     RSKKSVYVLASFGNSYHTDSGFLTWTLAQSFPVGKPLAVMINGGSAPAELTGKFFEVS
                     QRAVLAK"
     gene            complement(9299..11983)
                     /gene="ppc"
                     /locus_tag="PKL17_14085"
     CDS             complement(9299..11983)
                     /gene="ppc"
                     /locus_tag="PKL17_14085"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNK45913.1"
                     /translation="MLPTVTASSITQNADIRFLGKLLGDVIREHGGEALFRRIEDIRS
                     NSVDRYRGIVTADVVDRGLAGLGLDETVAVVRGFMLFSMLANLAEDRQNLTNRIDEDL
                     ATVLVRLGAQGIDPKEALRLLDGALVAPVLTAHPTEVMRKSMLDHRNRIAQLMKLRDH
                     GKSETPDGDLIESAIVRQIALLWQTRALRRERLYVKDEIETALAYMREVFLPALPALY
                     SRWERVLPHRPQSFLRLGSWIGGDRDGNPNVTASSLDQALRRAGATVLLSYLEQLDAL
                     AAELSISSELATVSDKVRALADASGDNLPSRSDEPYRRAIAGVYARLCATYVDVTGQK
                     PPRPSSLSATAYADPDSLRSDLLDIAHSLGGSRQTQLSQVGSLGRLIRAVETFGFHLA
                     TLDLRQNADVHARVVAELLRTAQVESDYMALTENDKVALLRRELASPRPLASPFAKYS
                     EETQSELAIVNMAQRVHATYGPAAIRHYIVSKTESVSDLLEVQILLKEAGLYRHDDSE
                     RAGIMVVPLFETIEDLEQAPAVMNKWLSLPEVAVRTKACGHQEVMVGYSDSNKDGGYL
                     TSVWGLHKATRGLAESFAQSGTRLQLFHGRGGAVGRGGGPSFAAILAQPSGTVQGRIR
                     ITEQGEVIAAKYGTKESAMNNLEAMAAATLLASLEKTTTFAPQKQRFFAAMEQVSQHA
                     FRCYRDLVYGTEGFRTFFRQMTPLREIAELKIGSRPASRTKSDRIEDLRAIPWVFGWA
                     QARVMLPGWFGVGQALQSFADVALLRDMAATWPFFRATLDNMEMVLAKSDLRIAARYA
                     SLVEDRELSATLFGRIRDGWQAAHDALLAITQQSRLLEKNPALDTSIRLRLPYIEPLN
                     LLQVELLKRYRSGEHDPRVKEGIHLSINAIATALRNSG"
     gene            complement(12123..14294)
                     /locus_tag="PKL17_14090"
     CDS             complement(12123..14294)
                     /locus_tag="PKL17_14090"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK45914.1"
                     /translation="MDRSFRIGVMLSAVFIGLGVATCNGSSIGEELQDAGVNDDLSVA
                     LGPACGGSNCQLGEVCEVGVCHKDCGAGGVRCSNPEVCCKSGDICYLDACTTPGQACT
                     AGSPDGGTGAGSACLPAVCPSGQYCESSIGKCLPLTTTGTCEYRPPTGQFSPKVKWAW
                     SGSSSLPTYNQIMMTPVVADLDGDCMPDIVFSTFAGANYGADGVLRAVRGDGSKELWT
                     VTDTTLRAVPGSQIALADVDNDGKVEVFACHQSGALMALNFDGTKKWLSTNKPCSAYD
                     APSVADLNHDGVPEVYVGFAVHNARTGAIMGTPPAVSAPYSVAAELDGNVANGMELVA
                     GGVVYHMDGSLYWNAGHGTGYPAVADLDGDGKPNVVSVVGATHAVYAYKHDGTLLWGP
                     KDVNNGVATPSGPSGGGTPTIADFNGDGMPDVATAGGYGYLVLNGQNGSVLWQSTATT
                     DTSSRVTGSSVFDFEGDGPAEAVYNDERNLRVYDGKTGKVLVKLCNTSGTLWENPVIV
                     DVDADDHAEIVVMANNYAINTCDTGGGSSTGFKVIGDTQNRWVRTRRIWNQHTYHVTN
                     VNDDGSIPTTEDNNWTKTGLNNFRQNVQTKNTLAAPDLVPRGLRQTFDLCTQGQIVIV
                     ATVFNQGAATAAAGVPVTFYAVDQSGTSTPIATILTTVPILAGGSQVLSTPWAPAGGS
                     RGPYQIRVVVDDDGKGNGSTNECNETNNASATFSVGCPSVG"
BASE COUNT         2847 a         4287 c         4313 g         3121 t
ORIGIN      
        1 gagcacaaac gtcggtggct gctgtggccg accgaccagc ttgaccgctg aattggcgcc
       61 gcgggtcaag tcgttcagcg acgggatgag cgccgacaga aggcccgacg acagcatctc
      121 gacttgcgcg gttccgacat ccagacacag cgcgcccgtg tcatacagcg tgtagccgac
      181 cgagttgagt tccagcgtcg agatgcccgc accgatgtga tacggcttgc cattgggcgt
      241 cgtgccaccc gcgaacgccg cgctcttgcc gggggccggt cccgtcgggg cgggccgcac
      301 agggacgcag gtgctgcggt tttgcgacgt ggtgccgccg agcatgccga gcgacattcc
      361 tgccgtcgga agcgcttcca ccgtcgcgta gttgcccgcc gctgcgtaca gatcgagcgc
      421 cgccgtcttg gccgctggag aatacgctcc gagtgcgctg ccgaggtcga tgcggccttc
      481 ggttccaatg agctgcaagc aagtcccgtc tcgcatgcaa tagttgttga cgcagccttg
      541 gttggccagc gacgagcagt ccggcttggt actgcacttt tgacacatca gcgaatcgag
      601 cggcgccgcc agttgtcgct tgagctggtc tttgagcgtg ccgatgaacg gccccttgat
      661 ccaatcaaga aggtcgcagc cgaagctgcc gctgatgtcg atgtcaccgc tgtcgaggtc
      721 gaggatgtcg acgctcttgt cgtcgaagtc gaagctggtg agcttggttg tggcgttgac
      781 gttggttgcg acggccatcg tcacaccgac gtccggcttg ccgctgcggg ccgtgtcgag
      841 cgccagcttg cagtcgaccg tgaccgggat cgggccgagt ttgactttgg ccttcacctt
      901 catcggttcc gacgttttga tcttcgcgcg cagcgtcagc gccgtcgtgg tgggcggctt
      961 ggggtcaaac ttgagcgact ggtagtcgag cttgactttg cacatctggc cacagcaaat
     1021 ttcggtgtca ccaccgcact gcgacggcac cgggatgtca ccaccgccgg gaagcagctt
     1081 ggtgacgatg gctttgccgt tcgtttccag gaagtcaaag aagcctttgg tgactcgcaa
     1141 ctggatcgcg ttgtcgatcc gtttgtcgac gggatatcca ttgggaatct tggttaggcc
     1201 tcccgcgccg caaccaccgc cgccgctgcc gccgcaggcg acgataaagc tgagcccaag
     1261 aaccgctgct gctcgtcgcc acggcagctt tttggggacg cgagttgaag atcgtgcttc
     1321 agaaccggac atcgccacct ccgagtgttt ttcgagtgaa acgggccaga atgagcggaa
     1381 cgtagcaaag cggctccatc gctctcaagc ggattgttga cccaaagtca gcatcggact
     1441 gcgaattgaa ctgcccctat ttgacgacgc cagattgagc agcgcagttc acaatatttg
     1501 cggaaagcaa agcggacgcc atagtatgga accgccatgt cgtctgccct gtttcagcga
     1561 gtcacccgaa acgttctgcc gtcgctacat ccgaccaaag aacagtgggt cgggtgtctt
     1621 atcggtcagt gcattggcga tgcggtgggc gcaccagtcg aaggtctttt gcccaaagat
     1681 tgcaagcgtc acgccgaaaa agtgctgcgg catgggctgc tggatgatcc gtttcttggg
     1741 cagacccgct ttggccagta caccgatgac agccagctcg cgcgggagct cttggcggga
     1801 ctttgtgagc atggcgaact aaacagtgct gactacgccg cgcggatcgt gcggctgttt
     1861 gtgcaaggtg aagtcgtcgg ggcgggcaaa gcgacggcgg aagttgcgca tcggctcgcc
     1921 cgtggggttg cttcagatga agcggggatt ccagcaccgt cagcaggcaa tggcagtgcg
     1981 atgcgtgcag cgccgattgg tctgtggtac gccgacgatc cggtgaagct ggtcgcggcg
     2041 gcgcgggaac agtcgcgcat cacccatcaa gacattcgct gcgccgcggg tgcggtggcg
     2101 attgccggcg cggtggcgat tgcgctgcgt ccgtcgccga tcgtcgtgga accgttcctc
     2161 gatgaactgg ccgagtgggt gcgctgcatc gacgaaaccg ttgcgtttga tgtaaatcag
     2221 gtggcgtcgt gggtgtcgct gtcccctgac gcggcagcga cgtttatggc gcgggctggg
     2281 ctctgtccag gtcacgatgg cgacggggaa tggcgtggca tttccggtta cgtggtgtcg
     2341 agcgtgctgt ggagtctgta cagttttttg cgcagcccgg aagactttgt acaaaccatc
     2401 gagaccgccg tttccgtcgg tggggacgta gacacaaccg cggcgatggc cggcgcgatg
     2461 agcggtgcac acctgtcgat agatgcgctg ccgatgaccc tgtgtgaacg gctgacggat
     2521 caggggcgct ggggtttcgg tgagctttcg cagcttgccg agagcgcttt ccatacctgg
     2581 caagcccagc ggcgcgctgt gtcgtagcgg gattacttgc tgtcggcggg ctcgtcggtc
     2641 agcatctcac cgtcgagatc gcgcgacaaa agcgagtagt cattggcggc caccgacgca
     2701 acaatccgct cggcgacttc atcaatggcg accgattggc gcttgaaatc gaggctgtac
     2761 cagaagatgg gcttgccttt ttcgacccag ccttcgatgc gcagcatcgt ctttgtgcgt
     2821 ccttgctgcg gattgccgta gtcgccgtgt ccctcgataa gcggaacatc ggcgcgggcc
     2881 gctcgcgtcg caaagcgcag gatcagatcg aacgtggccc cgcgtcgggc aatccgcacc
     2941 cgcatgccat cgcccgaatt ggcgtccccg agcgcgacgt tgggcgtttc gacccagctg
     3001 atggtcggaa tcgggtgtcc atcgggatgt cgcagcgact gattgaacag ccgcaccgct
     3061 tcgcgcagct cttggaccag ttgtagatag cgctctggca ccgacttcga cacgatctcg
     3121 gcgcgcattg cgttgtcgcg ttcgcgctgg gctgcgtcgg cagcgttttc actgtcgatc
     3181 cgcgcgatgt cgaggatgcg agcgcgtgct ttgtctggtg tctgttcgtc cacagccgca
     3241 aggactgctg aatcgctgac agaaggggct ggctcggatg ccgaaggatg ttccgccatg
     3301 gtcgaaaatt atacgcgcaa accgcagttt cctgacagcc ggcggattgc gccaaagtgg
     3361 ctcgacgctg tggactcttt tcgctgccca cagttgcgca tatagtcgcc gccatgcaga
     3421 agcacgcgcc gtctcttggg tcgtctggcc tgtcatccgc gccgtttccc gcatcgcaaa
     3481 acagcgacgg tgggtcggtc gcgatctgga cagatgtggg aatcggctgg ctggtgacgt
     3541 cgcttgcgct tttggcgctc aagtcgccga tgctaaacga tccgtacttc tgggacgagc
     3601 tcggctgtta ttttgcgcag gtctaccaga tgtcgacgcg gctttcggcg tatctggcgg
     3661 ccaagccgga atatgtgcgg tcgccgcttc ttacgtcgct gctttcgctg cttcatcact
     3721 ttttcggacc gtcgcgggtg cttcagcacg gtgcgatggt gctgatctgt tcgctggttc
     3781 caccgtcggc gtatgcactg actcggcagc ttggtggaac gaaaaaaatc gcgtttttgg
     3841 ccgcgcttct gtgttttgtg acgccggcgt ttttcgccca atcggcgatg gttcagatgg
     3901 acttgccagc gacgggaatc tgtacgatcg cgtttgtctg tctgctgcgg aaaaatctgc
     3961 tcggctttgc gctggccggc agcatggcgg tgctcatcaa agagagcacg tactatgtct
     4021 gtctgcccgc ggcgcttgtg gtgtacggtc gtttggtgct gttggatcgc cgcaagccgc
     4081 tggcgctgtc cacgattttg cggctgtggc cgacgggaat tcccggcatc acgctgtttc
     4141 tgtggctgct tgtgcatcgg cggctcaccg gagcgatgat ttccggcgat cacaccgcag
     4201 tgattgggct gcacggaatt ttcccgtcgc tgctccacaa ttttgtcgaa ggccgacgct
     4261 ttgcgctgtc gctgtgtgcg ctggcctatc tgttcgcgat gcggatgccg ctgggaagcg
     4321 agcagaaaat ccgggtgatt gcgacggcag tgctgtggat ggcgctgccc ttttgctttc
     4381 cgggtcatct ggttcgctat cttttgccaa gcttgccggc gctgtgtgcc ctggctgcgc
     4441 tcggggtgtc acttcttccg attcctcgtc gagccgggtt ggcaacgctg ctggtctttg
     4501 tgctggtgtc cggtttccgc ggcgattcct ttcacgaaaa tccgccgttt gaactggaag
     4561 ggaatctgtc ctatcggctc cttttgcaag aacaagtggc ggtggtacgc cgggtcgcag
     4621 cgtcgggaag caagctggtg attgctgatt ttccgtttgc caacatgttt ccggccccgc
     4681 gtgagttcgg ctatctggaa aactggattc cgacgcttcc gctgcatcga atgacaaagc
     4741 cggatgatct gtgccaaggc gatgtcgtgg tgatgaccag tgtgtcgcag gacaaagaac
     4801 cgcttatcga aagcgccctg cgggtgggaa tccttgtgcc ctggttcgtc ggtgcaggag
     4861 acaatttccg cgtcggaaac agcctgtttg tccccagctt tgcgcggcac gatctgcgaa
     4921 tccgggtcta tcgcgtaacc tgtccaccga agtaggcgac gcgctgcgat gttcgattcc
     4981 cactgtcact tgcatgatga ccgcagcttt gcgctggctc cggacatgat tgcgcgggcg
     5041 caacaggtcg gactgcgtgg acttgtgctt gcgggcgtgg atcgcgatac ctggggacgg
     5101 caggatgcac tgcgtcgtca gtttgggagt ccggactttt cgatcggagt cgcttacgga
     5161 gtccatcccc aactgattcc ttcgctttcc ccttctgcgc tggaagaaca gctcgcggcg
     5221 ctgcactgtg cggcgcgcgg aacgctggaa atcgatggga atttgatcgg aaaacccgat
     5281 gccatcggtg agcttgggct cgacgctgca gctgatcaga cacagcaaaa catgacgtca
     5341 cagacccatg cgtttcggca gcagcttgcc ctggcgcgtg atctggatct gccgattgtg
     5401 ctgcacatcc tgaagaccca tgcccaagtc cttcagatcc tgaaacaaga cggaattccg
     5461 cgcgcaggtg gtgtggtgca ttcctattcc ggctctgccg agcagtgtag agaatatgtg
     5521 cagcttggac tttgcatttc gtttgctggt ggggtgacgc gcgaacatgc gcccaagctg
     5581 tgggcttcgc ttgcgttggt tcctgaagac cgactcctcg tcgaaacaga tgctccggac
     5641 cagaccccga tggcacatcg tccggcccac aacgagcccg catttttgcc tgcaattttg
     5701 gcggcagttg cgaaacaacg gaatcagact gtgaagcaga ctgccatctt gaccgaacag
     5761 aatgcgcggc ggctttttcg tctgccgcgt taaacagaaa aagaacacac gatggatacg
     5821 acgcttgcac ttcctggcac ttcccttccg gaatccgatg acggtgaaac gcgctaccgc
     5881 ttgcaccgcc ggtttgatcg catgggacgc ctggttggcg acgaaaaaat ggagcgtttg
     5941 tttgcttccc atgtgctcgt gataggggtt ggcggcgtgg ggtcttttgc ggcggaatcg
     6001 cttctgcgca gcggcatcgg acagcttacg ctggtcgatt ttgatcgggt ctgcgtgacc
     6061 aatacaaacc gccagctcca agcgatgcgc ggcacggtgg gaaagctgaa agcgcaagtg
     6121 ctcgccgaga ggttggcgct ggtgaatccg caagccaagg tttccgtgat cagtcgattt
     6181 tatgattcag cgacgagcga attgatcttg gatcgcgacc cgcggcctga ttttgtgatc
     6241 gacgcgatcg acaacatcac ggccaaatgt cacctgatcg cagcgtgcaa gcagcgtgga
     6301 cttccgctgg tcagctcgat gggagcggcg ggtcgctgtg atcctactgc gatccgaatt
     6361 ggcgatttgg ccgacacccg gatttgtccg ctggcgcagg aagtccgcaa gattctgcgc
     6421 accaagtttg cttttcccca agaagggctc tttggcattc cctcggtatt ttccgtcgaa
     6481 aaaccgcgcg agccgatcga gcttcactac gatgatggga tgggctttgc gtgtgtctgt
     6541 ccgggcggaa aaaacgaaca ccattcctgc gaggaacggc gtgtgatcta cggaaccacc
     6601 agctacgtaa ccggcagttt tggactgttt tgcgccagtg tcgtcgtaaa ttcgctgacg
     6661 gaactgcgcg gcagcaagcc aggaagcgtc tgatgccccg tcgtgctaga cccgccgcag
     6721 cggcatgacg tctttttcgt tggcggacgc agctcggcct tccgcaagct tttgtccgtt
     6781 cggtaaaagc gtgcgcggaa ccggggctcc cgtcgcggca agtatgtatc gctcacacgt
     6841 cggatagcga tccgattcac agtatcgcat gacccagatg cgcagcagcg tcttggtctg
     6901 aaacaccttg aacatgggac atcgttcatt gtgcggacag gtcatggtca agtcctttgg
     6961 ggaagcgagc taatcacttt ggcaagatcc tgtatcgaag cgcagcacac gctcgtgcaa
     7021 gatggcgtac atccagtttc ctaggccaat gccgacaagc gtacagagcg ccatcgcctg
     7081 gccttcgcca agctgaatga agaccgcact aggacaggct ccgcacagcg cccagccgat
     7141 tccgaacagc aagccacctg caatcgtgcc gcgatgaatc ggtttggggg cccagtctgg
     7201 cttcgttttc gccagcttgg cgatgagttt ccagcttccg gcgagcagca gcacggccaa
     7261 aaaaaacgtg tagagcagtc tctgatccga aaagcggagc atcttgtgta gttcggtcat
     7321 cgacgaaaaa ccgatgcagc tgagcgcaaa tccgagagcc aacccgatga cgctgtttcc
     7381 gatgatgtcg cgattcatgg tcatccccca aacagaagtc gggtcaaagt ggctccgctc
     7441 aggaagaagg ctgccgtcgc cacaatgctg cctggctgaa gccgactggt gccacagagt
     7501 ccgtgtcctg atgtgcagcc acctgccatg cgcgtaccga ttcctaccag gattcctccc
     7561 ccgaaaagca gcagcgcttg tgcggcacgg gaattcccaa gcaggcgcgc agtcgcgtca
     7621 tcgcggagcg aaaaatcgac ggaaaaacgg ccatcgagaa ctgccgccag gaacccaccc
     7681 agaataagtg atgccaggaa aaccaggtga gcggacaggc tctgtggtgg tctctgttcg
     7741 ctggctacgc tttgtgcggc tggggggggt tcatggctct ctaaaacttc cgccagcgca
     7801 tcctttccga atgccgcgag cgttgctgct tccagctcct gtgccagctt tgcatcgtct
     7861 atgttgtcat ctgggacggc gccgaagcgg attctgttta cgatcgcgga atagcgaccc
     7921 gacaccccca acatccgtcc gatcgacagc caatgcgcta cgcagatcgc cgcgagcgtt
     7981 gttcctccca accagaaagg ccagttgtgc aacatgacag acccctgatt cccaagcagg
     8041 cagcgtttgc ctgttcccgt gttcattgta ctatgggaca cgccctattg cagcgattca
     8101 gcgacgggaa cttggcctac agagcgattc ttttcgtacc gcagatgtga aataggaaac
     8161 tggctctttg tgcaccacag cactgcatcg tgtgggaatc cgcaacgcgc caaaagaagg
     8221 gcagggcgga cagactcccc ggccatacat ttcgtgagtg atgcgcgctg cgacggcgtg
     8281 acatgcaagg acagcctcgc gtgtggccgc gcgactgcga tccggcgttc tgtcaatcag
     8341 gtgaaaaaag ctatcgccgc ccacgttgtc gccgtagcat tccgcccatg cgtgctctct
     8401 gttcctttct cttgttcggg acgttcgcgc ttgcgatgcc cgcctctgct tcggaaaaac
     8461 atgtcgccga agtgctggac cgcgtgctgc aaagcaagcg agcgctcgaa gcgagcccgg
     8521 cgtgcaaaga gggctgcgtg tttctgtccg catgggactt cgacggaacc atactcaaag
     8581 gggacgcgag cgaaggactg tccgaagaca accacgtcgt ctatccaggg cttgcgcaga
     8641 aatccatcga agccggcttt tccggtctgt acaaaaagac cgaatttcaa aaattttggc
     8701 acgattatga agaaatggac caaaagcagg gacatattcc tgcttatacc tatctgccca
     8761 aaaccatgaa aggaaccaag gcagatgaga taaagaaatt tgccggcaat tattatgcaa
     8821 cagtcttaaa gccttactat tttgaatctt ccatcgatat attccaaaaa ctgaaacaaa
     8881 gcggtgtgat tccgtatgtc atctccgcgg cgccacatgt ctttgtcagc agctcaggtc
     8941 cgacgctggg catcgacgaa cagcacattt tcgggatcga gctggcgctg accaaagacg
     9001 gtgtgctgac cgatcgggtg atcgatccga ttccgtatgc cgaaggaaaa gccaagcgac
     9061 tggcgatgat cgtcgaccag gaaaaagcgc gcagcaaaaa gtcggtctac gtgctggctt
     9121 cgtttggcaa cagctaccac accgattccg gcttcttgac gtggacgctc gcgcagtcgt
     9181 ttcccgtggg aaagcctctc gcggtgatga taaacggtgg cagcgcgccc gccgagctga
     9241 ccggcaagtt ctttgaagtg tcgcagcgtg ccgtgctcgc aaaataagcg caggctgttt
     9301 atccactgtt gcgaagcgcg gtggcgattg cgttgatgga taaatggatg ccttctttga
     9361 cgcgagggtc gtgttcacca ctgcgatagc gcttgagcag ctcgacttgc agcaaattga
     9421 gcggttcaat gtacggcaat cgcagacgaa tcgacgtatc gagcgcgggg tttttttcca
     9481 gaagcctcga ctgctgcgta atcgccagca gtgcgtcgtg ggccgcttgc cagccgtcgc
     9541 gaatgcgacc aaacagcgtt gccgatagtt cgcggtcttc gacgagcgaa gcatagcgcg
     9601 cggcgatccg cagatccgat ttggccagca ccatttccat gttgtcgagc gttgcgcgga
     9661 aaaacggcca ggtcgccgcc atgtcccgca aaagagccac atcagcaaac gactggagcg
     9721 cctgtccgac gccaaaccag cccggaagca tcacccgcgc ctgtgcccag ccaaacaccc
     9781 acggaatcgc gcgcaggtct tcaatccgat cagactttgt acgcgacgct ggacgcgatc
     9841 caatcttcag ctcggcaatc tcgcgcagcg gcgtcatttg gcgaaaaaat gtccgaaagc
     9901 cctctgtccc gtagaccaag tcgcgatagc agcggaaggc atgctgcgag acctgttcca
     9961 tcgcagcaaa aaagcgctgc ttttgtgggg caaacgtcgt cgttttttcc agtgaagcga
    10021 gcagcgtcgc cgccgccatc gcttccaggt tgttcatcgc gctctctttt gttccgtact
    10081 tcgccgcgat gacttctccc tgctctgtga tgcgaatccg tccctgtacc gttcccgaag
    10141 gctgcgccaa gatcgccgca aaagagggac cgccgccacg accgactgca ccgccacgtc
    10201 cgtgaaacaa ctggagccgg gttccgctct gcgcaaagct ctctgcaagt cctcgcgttg
    10261 ctttgtgcag cccccacacc gacgtaagat agccaccgtc tttgttggaa tcggaatatc
    10321 cgaccatcac ttcctgatga ccgcacgctt ttgtgcgcac cgcgacttcc ggcaaagaca
    10381 gccacttgtt catcaccgcg ggcgcttgct ctaggtcttc gatggtctcg aacagcggca
    10441 ccaccatgat tcctgcgcgc tcgctgtcat cgtgccgata cagacccgct tcctttagca
    10501 gaatctgtac ttccaaaagg tccgacacgc tctcggtctt cgatacgatg tagtgacgga
    10561 ttgcagccgg accatacgtg gcatgcaccc gctgcgccat gttcacaatc gcaagctcgg
    10621 actgggtttc ctcactgtac ttggcaaacg gactggcaag cggtcgcgga ctcgcaagct
    10681 cacgacggag cagcgccact ttgtcatttt ccgtcagcgc catgtagtcc gattcgactt
    10741 gcgcagtacg cagaagttct gcgaccacac gcgcatgcac atcggcgttt tgccgcagat
    10801 cgagcgtcgc caagtgaaag ccaaacgtct cgaccgcgcg aatcagacgg ccaagcgatc
    10861 ccacctgtga aagctgtgtc tgtctgctgc cgccaagcga gtgcgcaatg tccaaaaggt
    10921 cactgcgcaa gctgtctgga tcggcataag ccgtcgctga caagctggat ggccgcggcg
    10981 gtttctgtcc cgtcacatct acataggtgg cgcacaggcg ggcatacaca ccggcaatcg
    11041 cacggcggta gggctcatcg ctgcgcgacg gcaggttgtc cccgcttgcg tcggccagcg
    11101 cccgcacttt gtcgctcacc gtggcaagct cgctcgaaat cgacagctca gccgccagcg
    11161 cgtcgagctg ttccagataa ctcaagagca cggtcgcgcc cgcccgccgc agcgcttgat
    11221 cgagcgacga cgccgtgaca ttgggattgc cgtcacggtc gccgccaatc cagcttccca
    11281 atcgaagaaa gctctgcggt ctgtgcggca gcacccgttc ccagcgtgaa tacagcgccg
    11341 gcagcgcggg caagaacacc tcgcgcatgt aggcgagcgc ggtctcgatt tcgtctttca
    11401 catacaggcg ttcacgacgc agcgcccgtg tctgccacaa aagagcaatc tgccggacaa
    11461 tcgccgattc gatcaagtct ccgtcgggcg tttcgctttt gccgtgatcg cgcagcttca
    11521 taagctgggc aattcggttg cgatgatcca gcatgctctt gcgcatcact tcggtcggat
    11581 gtgccgtcag aaccggagcc accagcgcgc catcgagcag ccgcagcgcc tctttcggat
    11641 cgatcccttg cgcaccaagt cgtaccagca cagtggcgag atcctcgtcg atgcggttcg
    11701 tcaggttctg gcggtcttcg gccaggttcg ccagcatcga aaacagcatg aagccgcgca
    11761 ccaccgccac ggtttcatca agcccaagcc ccgcaagtcc tctgtcgacc acgtccgccg
    11821 ttacgatgcc gcggtatctg tcgaccgagt ttgatcggat gtcttcaatg cggcgaaaca
    11881 gtgcctcgcc gccgtgttcg cgaatcacgt ctccgagcag cttgcccaag aaacggatgt
    11941 cagcattttg ggtaatggat gaagcggtga cggtcggaag cacgcgatct cctggcagaa
    12001 tccggctgac caaactctac cagacccgtg cgggggcaag cgggcggcag cgcaaaaacg
    12061 gtgtcaggtt ctgggctcat accttcatct ggacggtgcc agccgcccag gttcacgact
    12121 tgtcagccta cgctcggaca acccacagaa aacgtcgcgg aggcgttgtt cgtttcgttg
    12181 cactcattgg tgcttccgtt tccttttcca tcgtcatcga cgacgacgcg aatctgatag
    12241 gggccgcgcg aacccccagc tggtgcccat ggcgtggaca acacctgcga tccaccggcc
    12301 aaaatcggaa cggtcgtcag gatggtcgca atgggcgtgc tggttccgct ttgatcgacc
    12361 gcatagaatg tgactggaac gcctgctgct gcagtggctg caccttgatt gaacaccgtc
    12421 gccacgatca caatctgtcc ttgcgtgcaa agatcaaacg tctggcgcag tccacgcggt
    12481 accaagtctg gcgccgccaa ggtatttttg gtctgaacgt tctgtcggaa gttgttgagc
    12541 cccgtcttgg tccagttgtt gtcttccgtc gtgggaatgc ttccgtcgtc gtttacgttg
    12601 gtgacgtggt aggtgtgttg gttccagatt ctgcgcgtcc gcacccagcg attctgggtg
    12661 tccccaatca ccttgaatcc cgtcgatgaa ccgccacctg tgtcacaggt gttgatggcg
    12721 tagttgttcg ccatgactac gatctctgcg tgatcatctg catccacatc gacgatgact
    12781 ggattttccc acagggttcc agacgtgttg cacagcttca ccaggacctt tcctgtcttg
    12841 ccgtcatata cgcgcaggtt ccgttcgtcg ttatagacag cctcggcagg cccatctccc
    12901 tcaaagtcaa aaacagagga ccccgttacg cgcgaagagg tgtctgtggt cgcggtcgac
    12961 tgccacagaa cggacccatt ctgtccgttt aggaccagat agccgtatcc tcctgccgtt
    13021 gccacatcag gcatcccatc cccgttgaag tctgcaatgg tgggcgttcc accaccggac
    13081 ggacccgacg gagtcgcaac gccattgttg acgtctttgg gaccccagag cagcgttccg
    13141 tcgtgcttat acgcatagac tgcatgcgtt gctccgacca cggacacgac gttcggtttg
    13201 ccatcgccgt caagatccgc cacagcggga tagccggtac cgtgccccgc gttccagtac
    13261 agcgagccgt ccatgtggta gacgacacct ccggcgacca gttccatgcc gttggcgaca
    13321 ttcccatcca gctctgcggc gacactatac ggtgcagaga cggctggcgg cgttcccatg
    13381 atcgccccgg tccgcgcatt gtgcaccgca aaaccgacgt agacttcagg aactccgtca
    13441 tgattcaagt ctgcaaccga aggtgcgtcg taggccgagc atggcttgtt ggtggacagc
    13501 cacttcttgg ttccatcgaa gttcagagcc atcagggcgc cactttggtg gcaagcaaac
    13561 acttccacct tgccgtcatt gtctacgtcc gccagcgcga tttgggaacc aggcaccgct
    13621 cgcagcgtcg tgtcggtcac agtccacagc tctttgctgc catcgccgcg caccgcccgc
    13681 aggacgccat ctgctccgta gtttgctccg gcaaatgttg aaaacacaat gtcaggcatg
    13741 cagtctccat cgagatctgc caccaccgga gtcatcatga tctggttgta cgtagggaga
    13801 ctgctcgatc cgctccaggc ccatttgacc ttgggagaaa actggccagt cggcggtcta
    13861 tattcacagg tgcccgttgt cgtcagtggc agacacttgc cgatggatga ctcgcagtac
    13921 tgtccgcttg gacagactgc cggaagacat gcagacccag cccctgtgcc accgtctggc
    13981 gatcctgcgg tacacgcttg gcccggagtg gtacaggcgt ccagatagca gatgtcaccg
    14041 gacttgcagc agacctctgg gtttgaacag cgcacgcccc cagcgccgca gtctttgtga
    14101 cagaccccta cttcgcagac ctcgccgagc tgacagttgc tgccgccaca ggcaggaccg
    14161 agtgcgacac ttagatcgtc gttcacacca gcgtcttgca gttcctcgcc gatcgatgat
    14221 ccgttgcaag tagccactcc caaaccgata aacaccgccg acaacatgac accaatgcga
    14281 aacgaacgat ccatgatgcc cccttctggt tcggaaaata caaagcacac aaaaagacaa
    14341 cctaagaata catttttttg aggatggcag gcaatcgcgc gcaagggaac ggcgtcagcc
    14401 ggccaactcg cagccggaca acagactgtg ccggtcaatt gcagacaacc ggcagtgcgt
    14461 ggggggggaa aggaggggga aagactactt cgcttcgtga taaccgatgt cgggggctgt
    14521 accaagcgga cgaacgcccc caaagaagtc ggtggtgacg gtcggatc
//
LOCUS       DAORGN010000129        14509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1715_length_14509_cov_6.083506, whole genome shotgun sequence.
ACCESSION   DAORGN010000129 DAORGN010000000
VERSION     DAORGN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14509
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1715_length_14509_cov_6.083506"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKL17_14095"
     CDS             complement(<1..240)
                     /locus_tag="PKL17_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45915.1"
                     /translation="MKTVSAALKVGLTTLAILALSAVAYRFVAKGVRGQEGPVVWALF
                     RDATGLVDKSRVQIAGLIIGEIAERRLQGTYARVSI"
     gene            complement(254..1147)
                     /locus_tag="PKL17_14100"
     CDS             complement(254..1147)
                     /locus_tag="PKL17_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK45916.1"
                     /translation="MAVNKWQPSDEDASVRSERFHIRVRGINKTFGPHHVLRGIDLDV
                     ERGKINVIIGGSGQGKSVIMKHLMGLLKPDTGHIYCDGEDLVPLDEFQLNRLRKKFGM
                     LFQYAALFDSLTVEENIVFPLVEHGDPDSDGTDERGKPKKRRFFSRAELNKIAVTQLD
                     KLALPQVLLRKFPSELSGGQRKRVGLARALVMKPQILLYDEPTTGLDPVSTKNVDDMI
                     RDVSREHGVTSVIISHDMASTFRIGDRISALYGGVIIDSGAPEEIRKSRHKWLREFIE
                     TSGAVQMVPCDDKDLIVDEPI"
     gene            complement(1151..1969)
                     /locus_tag="PKL17_14105"
     CDS             complement(1151..1969)
                     /locus_tag="PKL17_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK45917.1"
                     /translation="MWLERLAAFLDRIWLPPVRLFLSLVEVLGQHLLLLFAVVRWALR
                     PPYRIGVLLEAMVFIGFESLPIVLLISLFVGAVFALQLSSALRTFQAQAFTGATVGLA
                     LARELAPVFTSIVVAARAGAGMATELGSMRITEQIDALATMAVNPVQYLIVPRVIAGT
                     VMMPMLAMLFNVVGMLGAYTVAVGVQNVDRGVFIEKAQWLLDPNDITQGMVKSVVFGC
                     VLTLIACQQGYNASGGAKGVGVATTRSVVGSFVTILVLDYFLTDMWLTLFTKKG"
     gene            2141..4318
                     /locus_tag="PKL17_14110"
     CDS             2141..4318
                     /locus_tag="PKL17_14110"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45918.1"
                     /translation="MKRNTLKNWILAPLFLLATATCSGNDISGQDQCASGVDTDGDGL
                     NNDIECLRGTDSNNPDSDGDGALDGAEVAAGSDPMKTDSDGDGLSDGAELAYPKICAA
                     TDPFNQRRPPPACTSGDQCQAGEVCQGLDPTKPDSDGDGVADAEEDKNLDGTITVLSG
                     ETDPRLWDTDGDHQSDKSAGSSICRPDGLATVNQAILQNASIQAGTDPALGTGKAFQG
                     ASGSGLAFDDGAAAVSGLVVNRTTTLANIDADRVDIEGKITTALTGKGYTVTSVFIGR
                     KFQTHELNQATQSTFRLVKAAVTGSTVRDDIVNSLSGATAPGGGSVTTQGPFYLDVTV
                     VRRDAAKTLDVILAVSPTALYDDRTKPAAIRAGDLTNASGIAISTKQISFICDGIQTT
                     KGSTADIVWTVDVSASMADNQVRIANTATEFFKRLQSAGVDFRVGIFTADSTIDPKLS
                     ATTYQSWPNGFKFISGTDPDGPHQVCRQVTAPNSGTAGFCPQDMNKTNDPIGPFGSTS
                     ASNANEEPAAAVVRLNDVFKKNAVANVTNADWKWRPGVTKVSFFVTDEYNNDWTRYFS
                     TANNPDTGKVWGTPYSATVLQGIGDYFRQNQILAFGAVPVTATRQCSANNSNDLPRCL
                     IENLGGAWIDINTALDADIAAAMNKLVDAIAGASSQFKLSRTPITSTIKVTVRGVPVP
                     RSRSDGFDYDPASKSVVFYGNTYRPQIGDLVFMSYRVWIGSLG"
     gene            complement(4373..4621)
                     /locus_tag="PKL17_14115"
     CDS             complement(4373..4621)
                     /locus_tag="PKL17_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45919.1"
                     /translation="MHNVTIAMTEPGVTRADLAKLLAQALGNEKAYDAVSKAAQKLGL
                     RREKLDKNEALSVLELIAQTPGLVGITARFAKSRFHLR"
     gene            complement(4643..5827)
                     /locus_tag="PKL17_14120"
     CDS             complement(4643..5827)
                     /locus_tag="PKL17_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNK45920.1"
                     /translation="MIETKVATALAAGSATEVADKLIAKVRSDLGELVPKLLMVFAST
                     KQPLGELMPLITAAFPHSTTLGASTAGEFIETGDAKSSACIFALAGDYRIFAGVGTAL
                     RASAEQSVAAALEGMPTQVVGYPHRTAILLLDPLSGSSEEATLITAALMGFDLRLAGA
                     AAGDDLALKSTQVALGNQVISDGAIVATIFSKQPLGVGVCHGHEPLSQPLTVTRAEGS
                     VVHEIDGRPAWDVWVERTRAATTKLGFDPTQIPYNDVGGFLLRFEAGLSQGDAFKICA
                     PLFRVGDHSIGFACGIPEGSVIRITESEPSRQIDSAREAARRARQQVGDVPLAGAVVF
                     DCICRNLILKDDFKTAIAGIHTELGQVPLAGFETYGEIALDAGDLSGFHNTTTVVLAF
                     PK"
     gene            6010..8604
                     /locus_tag="PKL17_14125"
     CDS             6010..8604
                     /locus_tag="PKL17_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK45921.1"
                     /translation="MREMLKHRSLGLRRTEMSMRKALWIGLSLLLGVPKGVSAEEPTG
                     TATTVTPVSPPAVSPPPTVAAPPAQPADSPPVPPAIEPDGEPPPLGYLSDGTRQKPKD
                     AVPFAGVVREKGSRDPLPAIRVTVTPWRENAPAPSDKPGKKAKPRGEAAGEPIEVTTD
                     AEGNFLVEDLAPGMYQVLLRGGQIQRTESIETLAAGKKTSITYYAPRRTNQFELVVRA
                     EPVKKEVSEQVLSVQELKRIPGTQNDAIKAVQNLPGVARAPFGGGLLVVWGSAPADTR
                     VYADGVQLPRIFHFAGFRSTINSEFVDELSFKPGAYGADFGRGLGGMIDVTTRSPKSD
                     RIHGSVTLDLIDGSVTLEGPVTKNLYVAGGVRVSWISVFLPIFNRSQFQVSPFYWDYQ
                     LSMRYRPTKADDIDVFIFGSTDNLEARVSDPDPAVNISLDTRQYFSRARVRWTHRFSS
                     STQLIVMPSIGGDVTRFDTGDGGIGSVSFKLEGFTLGYNLRSELRHRFAPWLNVIGGV
                     DFEGSRSSFEVVVPPIPPGGGGSSGETNNQTSGSAITQLGSVRDQSVLHIVRTAPYAI
                     ARFDFLDRRLTVSPQIRLETAYIRSYDGDVSRTVFSPEPRLVISGQVVPKYLLLKFGV
                     GAYSQLGQPQELNRVFGNPNLDPQRGTTYVAGFESEPTGSLFVQGQFFYKDLRSLIVS
                     DPVLKYTNAGLGRVIGGDFLLRQKLWKGLFGWLAYTISQSERKSSPQDPWRLFRYDQT
                     HILTAVASYKLPWFGIEVGVRFRYVTGGLYTPTTGAIRDTLTQTWVGTQGDVYSARLP
                     DFHQLDLRVDKTWTFNRWKLGLYLDIQNLYNRQNAEQLAYGGRQLYQTTLVTGIPFFP
                     NFGLRADF"
     gene            8617..9696
                     /locus_tag="PKL17_14130"
     CDS             8617..9696
                     /locus_tag="PKL17_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45922.1"
                     /translation="MTTKNRTRFATALALGLAMLAGCDNDIDPDTLARDIRVLSLRVG
                     SATPLSPAEAQAEVKPGPSGLDLVFTSDHLDLTANVAAPTGPGRRITAPRPLVYEWYL
                     CVGPASLFSPGTLDPGCRKWQPGDPDPMKSTSLRYLGSGANFSLPTAVLKDVVGGVLQ
                     ILLTGGSGGGSGGTVTIPDKPISILLPLIMRVRAQNGDPNDIRDSEVGVTYLRTWIAL
                     PGMTLPPVNQNPALADLVASADKDGTKKPLLPCTATSCPRNPVKRGADLFLVGASLTG
                     SIETYTPVDDTGRGARQEALRYSWFSTDGAYERERTGDTQPDNTWNSDNKRPPPDGIV
                     QSSLWLVVQDERGGGDCARYELDLN"
     gene            9724..10641
                     /locus_tag="PKL17_14135"
     CDS             9724..10641
                     /locus_tag="PKL17_14135"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK45923.1"
                     /translation="MSTKPVKTKPAPPREPLFHYKPSTKTPRPAKPRTRKAASSPSEL
                     SLIWGLLYDLIRAPGFLILLLIGRADPKAALVPFHRLWSFALSARATATLIVMNVLVF
                     AVELFLQPRWPSARFWSTFAFSPQALWQGQLLPAVLHVFSHASVPHLLSNMLALFVFG
                     RVVESLLGPWRMLGAYATAAVAATLVSLLAQGLSGQYVPTLGASGAVAGLIALGVLLS
                     PLSITFEALVPMPLFLVGWIAMAGDVLALWAKAKDSVDHPAHVGGYLSVVLVYRLLPH
                     KLKAKARFGLLLNVLTVLFALVLWYVRTP"
     gene            10697..13264
                     /locus_tag="PKL17_14140"
     CDS             10697..13264
                     /locus_tag="PKL17_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNK45924.1"
                     /translation="MARQIIVQRKSTLSTLQVRHPATLLIVAGLLTLLAVGYALRLRV
                     LTGFESLLPESRPSVLELKRVASLTSGVSTMFIVLEGNGQTPVAKLRAAADALVPKLQ
                     ELGPPWVGHAESGVQEALRFLRPRAGMFLPLPKLQELHDDIEERTAKALGEASGLFVD
                     LEDKPSEPLTRESLKKRLELDHEQTDRYPDGYYQSRDGQTLVVLVRSKIHGSDFDRGA
                     EAIARISAVVNRVNPASFHPSIRVGYSGDLVTAVSEYQAINRDLTDVGLFGSVLIGGV
                     VFLYYLRLRTVLVMLLTIGVGVAWTFGATQLVVGSLNVATGFLFTIIAGNGINFGIIY
                     MARYLEARRVGEALESALSTAARETWIPTLTAGFAAAASYGSLGATDFRGFHDFGLIG
                     GMGMLLCWLATFGVMPCLLTVFERLIPLDRDFGGPLSWLRKQTRGGGVAFSRPFELLV
                     ARAPRFITATGLLLTALGLFGTVKWLRSDPMEYDLRNLRTDVSSRADEIRLNKLGDEI
                     TGHVGATGMAILVDRPDQVQELTQVLLARRAAAPKGKEPFERLHALQDFVPTDQAAKL
                     PLLVRIRKRLVRAHEHGAISDENWQDIEPMLPPADLQPFGIAALPEGLARAFTESDGT
                     RGRIVYIAPTATASVDDAHYLFRWADSYREARLSDGSLIRGSGRAVIYADMWAAIVHD
                     VPIAVVLSFAATVLVVVLAFRGGRSSFAVLGSLWAGVFWMIGLLVVLKVKLNFLNFIA
                     LPITFGIGVDYSVNVVQRYVREGRGSALVAIRETGGAVILCSLTTILGYLALVRSTNF
                     SVRSLGVAAFLGEICCLLASVLLLPAALVWIDRRHKDAAASQPSPPDGPPSSGPFYT"
     gene            13298..14440
                     /locus_tag="PKL17_14145"
     CDS             13298..14440
                     /locus_tag="PKL17_14145"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK45925.1"
                     /translation="MTYLSLVLFVLATVGVLCLAIQTGFLWRFLAAKVRVPTQPLPPI
                     SILKPLSGCDDGLLANLESFAALDHPDYEVLLGVHTIDDPAVSVAQKLIDKHPQRFRL
                     VVQDGSPGLNPKVNQLITLAKEARHDVLLVSDASVRVGNGYLPEIAACLEDPDIGLVT
                     HIFAAEGEQSLGSLLDNFHLMSHYGGGAIAARDAGGQTLVNGKSTAFRRADLDAMGGF
                     AAVCDYAAEDFVLGLWVGRKIKKKVCFARYTPITLSVNRTLGEYIRRYQRWSIMQRTG
                     GGYLVYLSQTMVYPLILASLGLLFQPSLRGLFWWLGIGFVKAVLDYIQASMLRYKRIS
                     PLLMLLGPINDLLVAWIWTYGLLWNRTVWRGTQLKVTWGTKLVPLS"
BASE COUNT         2650 a         4321 c         4535 g         3003 t
ORIGIN      
        1 gatgctgacg cgggcatagg tgccttgcag ccgtcgctcg gcgatttcgc caatgatcag
       61 gccggcgatc tggacgcgcg acttgtcgac gagtccagtg gcgtcgcgga acagcgccca
      121 gacgaccggg ccttcctgcc cgcgtacgcc tttggcgacg aagcgatagg caaccgcact
      181 cagcgccaag atggcgaggg tggtgaggcc gactttgagc gccgcgctga cggtcttcat
      241 gggtaagctc cgcttagatt ggttcgtcca cgatgaggtc tttgtcgtcg caaggcacca
      301 tctgaactgc gccgctggtt tcgataaact cacgcagcca cttgtggcgg cttttgcgga
      361 tttcttcggg ggcgccgctg tcgataataa cccccccgta cagcgccgag attcgatctc
      421 caatgcgaaa tgtcgacgcc atgtcgtggc tgatgatcac gctggtgacg ccgtgttcgc
      481 gtgacacatc gcggatcatg tcgtcgacgt ttttggtcga taccggatcg agtcccgtcg
      541 tcggttcatc gtaaagcagg atctgcggct tcatcacgag cgcccgtgcc aggccgacgc
      601 gcttgcgctg accgccggac agttccgacg ggaatttgcg caaaagcacc tgcggcagcg
      661 cgagcttgtc gagctgggtg acggcgattt tgttgagttc agcccggcta aaaaagcggc
      721 gttttttcgg cttgccgcgt tcgtccgtac cgtcgctgtc gggatcgccg tgttcgacca
      781 gtggaaagac gatgttttct tcgacggtca gtgagtcaaa cagcgccgca tactgaaaca
      841 gcatgccgaa ctttttgcgc agccggttga gctgaaactc gtcgagcggc accaggtctt
      901 caccgtcgca atagatgtgc ccagtgtcgg gttttagaag ccccatcagg tgcttcatga
      961 tcaccgactt gccctggccc gatccgccga tgatgacgtt gattttgccg cgctcgacgt
     1021 cgagatcgat gccgcgcaga acgtggtggg gcccaaacgt cttgtttatg ccacggacgc
     1081 ggatgtggaa gcgctcgctg cgcacgctcg cgtcttcgtc ggatggttgc cacttgttga
     1141 ccgccatcga ctagcccttc ttggtaaaca gcgtcagcca catgtcggtc aaaaagtagt
     1201 cgaggacgag gatggtgaca aagctgccga cgacggaacg ggtggtcgca acgccgacgc
     1261 ctttggctcc gcccgaagca ttatagccct gctggcaggc aatcagcgtc agcacgcagc
     1321 cgaagaccac cgatttgacc atgccttggg tgatgtcgtt cgggtcgagc agccactgcg
     1381 ctttttcgat gaagacgccg cgatcgacgt tttgaacacc gacggcaacc gtgtacgcac
     1441 cgagcatgcc aacgacgtta aacagcatcg ccagcatcgg catcatgacg gttccagcga
     1501 tgacgcgcgg gacaatcagg tactgcaccg gatttacggc catcgtcgcc agtgcgtcga
     1561 tttgttcggt gatgcgcatc gaaccaagct cggtggccat gcctgcgccc gctcgagcag
     1621 cgacgacgat tgacgtaaac accggggcca gctcgcgcgc caaggcaaga ccgacggtgg
     1681 cgccggtgaa ggcctgggcc tgaaaggtcc gcagcgcgct cgagagctga agcgcgaaca
     1741 ccgcaccgac gaacagcgag atcaaaagta cgatcggcag cgactcgaaa ccgataaaca
     1801 ccatcgcttc cagcagcacg ccgatccgat acggcggacg cagcgcccag cggaccaccg
     1861 cgaacaaaag cagcagatgt tgccccagca cctcgacgag actgagaaag aggcgcactg
     1921 gcggcaacca gattcggtcc aaaaacgccg ccagccgctc aagccacatg gctccatgct
     1981 accgcaaagc gaggctgcac attcgctgta tcccggcgca ggcggccagg aaaaaagcaa
     2041 aagccccact ttgcgcgcgc ggcctttaac ataaaaagac cggctgtcgt cggacgaggc
     2101 gggccgggtc tggttttcga cctttgcata tgggggcaac atgaaacgaa atacgctaaa
     2161 gaactggatc ttggcgccac tgtttctgct cgcgacagcg acatgcagtg gcaatgacat
     2221 ctcgggacag gaccagtgcg cgtcgggggt tgataccgac ggtgatggcc taaacaacga
     2281 catcgagtgc ctgcgcggta ccgattcgaa caatcctgac tcggacggtg acggcgcgct
     2341 ggatggcgcc gaggtcgcgg cgggttccga cccgatgaag accgacagtg atggcgacgg
     2401 actgagcgat ggagccgagc tggcctatcc aaaaatttgc gccgccaccg atccgttcaa
     2461 ccagcgtcgg cctccaccgg cttgcacgag cggcgaccag tgccaagcag gagaggtctg
     2521 tcagggtctt gatccgacca agccagattc cgatggcgat ggtgttgccg atgctgaaga
     2581 ggacaaaaac ctcgacggaa cgattaccgt attgtcgggt gagaccgacc cgcgcttgtg
     2641 ggacaccgac ggcgatcacc agtcggacaa gtcggcgggc tcgtcgattt gccgtcctga
     2701 cggactggcc acggtaaacc aagcgattct gcaaaatgcg agcattcagg ccggcaccga
     2761 cccggcgctc ggtaccggca aagcgttcca aggcgcgagc ggcagtggct tggcgttcga
     2821 cgacggtgct gcggcggttt ccgggctggt ggtcaatcgc acgacaacgc tggccaacat
     2881 cgacgccgat cgcgtcgaca tcgagggcaa gatcaccacg gcactgactg gcaagggcta
     2941 caccgtcacc agcgtgttta tcggacgcaa gttccagacc cacgagctta accaggccac
     3001 gcagtcgacc ttccgcctcg tcaaagcggc ggtcacggga tcgacggtgc gcgacgacat
     3061 cgtaaacagc ctgagcggcg cgacggcacc gggtggcggt tcggtgacga cgcagggccc
     3121 gttctacctg gacgtgacgg tggtgcggcg ggatgcggcc aagacgctgg atgtcatttt
     3181 ggcggtttct ccgacggcgc tttatgacga tcggaccaaa ccggcggcga ttcgcgcggg
     3241 cgacttgacc aatgcgtcgg gcatcgcgat cagcaccaag cagattagct tcatctgcga
     3301 cggcattcag acgaccaagg gttcgaccgc cgacattgtc tggacagtcg acgtatcggc
     3361 ctcgatggcc gacaaccagg tgcggattgc caacaccgcg acggaatttt tcaagcggct
     3421 ccagtcggca ggcgtcgact tccgcgtcgg tatcttcacg gctgacagca ccatcgatcc
     3481 caagctgtca gcgacgacgt atcagagctg gccaaatggc ttcaagttca tttcgggcac
     3541 tgatcccgac ggaccgcacc aggtctgccg tcaggtcacg gcgccgaact cgggcacggc
     3601 tggcttttgc ccgcaggaca tgaacaagac caatgatccg atcggaccgt ttggctcgac
     3661 gagcgcttcg aacgccaacg aggaaccggc ggctgcggtg gtgcgtctca acgacgtgtt
     3721 caagaagaac gccgtcgcca acgtgaccaa cgctgactgg aagtggcggc ccggtgtcac
     3781 caaggtgtcg ttctttgtca ccgacgaata caacaacgac tggacgcgct acttttcgac
     3841 ggcaaacaac cctgacaccg gcaaagtgtg gggcacaccg tactcggcga cggtgctcca
     3901 gggcatcggc gattacttcc gccagaacca gattctggcg ttcggtgcgg ttcccgtcac
     3961 ggcgactcgg cagtgcagcg ccaacaattc gaacgacttg ccgcgctgcc tgattgaaaa
     4021 cctcggcgga gcctggatcg acatcaacac cgcgctcgac gcggacattg ccgccgcaat
     4081 gaacaagctc gtcgacgcga tcgccggtgc atccagtcag ttcaagctgt cgcgcacgcc
     4141 gattacgtcg acgatcaagg tcacggtccg tggtgtgccg gtgccgcgct cgcgctcaga
     4201 cggttttgac tacgatccgg cgtcgaagtc ggtcgtgttc tacggcaaca cctatcgtcc
     4261 gcagatcggc gatctggtgt ttatgtcgta ccgcgtctgg attggctcgc tcggctaagc
     4321 gaaaaagaaa aagcggtgct gcctgcgccc gcgtcttgcg ggccaggtgg tgctagcgaa
     4381 ggtggaatcg gctcttggca aaacgcgcgg tgatgccaac gagccccggc gtctgcgcga
     4441 tcagctccag cacactcagc gcttcgtttt tgtcgagctt ttcccgacgc agccccagct
     4501 tctgcgccgc tttgctgacc gcatcgtagg ccttctcgtt gccgagggct tgggcaagga
     4561 gcttggcgag atccgctctc gtaactcccg gttctgtcat tgcgatcgtc acgttatgca
     4621 cgtgtcccct cgatgttcgt cgctactttg ggaatgccag gacgaccgtc gtggtgttgt
     4681 ggaaccccga cagatcgccc gcgtccagcg cgatttcgcc atacgtttca aaaccagcga
     4741 gcggaacctg gcctagctcg gtatggatgc cggcaatggc cgtcttgaag tcatctttga
     4801 ggatcaagtt gcgacaaatg cagtcgaaca cgaccgcgcc agcgagaggc acatcgccga
     4861 cttgctgacg ggctcggcga gcagcttcgc gggcgctgtc gatttgacgt gacggttcac
     4921 tttcggtgat gcggatgacg ctgccttcgg ggatgccgca ggcaaaacca atcgagtgat
     4981 ctccgacgcg aaacaggggg gcgcagatct tgaatgcgtc gccttgcgaa aggccagctt
     5041 cgaaacgcag cagaaaccct ccgacatcgt tgtacggaat ctgcgtcgga tcgaagccaa
     5101 gcttggtggt ggccgcacgg gttctttcga cccagacatc ccacgcgggg cgaccgtcga
     5161 tttcgtggac gacgctgcct tcggcgcggg tcacggtgag cggctgcgaa agtggctcgt
     5221 gtccgtgaca gacgccaacc ccaagcggct gcttcgagaa gatcgttgcg acgattgcac
     5281 cgtcagagat gacctggttt ccgagcgcga cttgtgtgct cttgagggcc aagtcgtcgc
     5341 ccgccgccgc gcccgccagt cgcaggtcaa agcccatcag tgctgccgtg atcagcgtcg
     5401 cttcctcgct gcttcccgaa agcggatcga gcaggagaat ggccgtgcga tgcgggtagc
     5461 cgacgacttg ggtgggcatg ccttcgagcg ccgccgcgac gctttgttcg gcacttgctc
     5521 gcagcgccgt accaacgccg gcaaaaatgc ggtagtcccc ggccagagcg aagatacaag
     5581 cggaactttt ggcatcgcca gtttcgataa actctccggc ggtggatgcg cccagcgtcg
     5641 tgctgtgtgg gaacgccgcg gtgataagcg gcataagctc gccgagcggc tgtttggtcg
     5701 acgcaaagac catcagcagc ttgggcacaa gttctcccag atcggaacga actttggcga
     5761 tcagcttgtc cgcgacttcg gttgcacttc ctgcggcaag cgcggtcgca acttttgtct
     5821 ctatcatccg gttttccccc ctatatctga cgctagaaga tgtgatcgcc gggtcgcaag
     5881 cgagcctttt atctggccga acttgctgtg gaaatggggg cagacggatt ggtcataccg
     5941 acgagaaggc tgtggagttt ccacacactg gtggcggtct ttggccgtcc tgctagtttg
     6001 atgcatcgca tgcgtgaaat gctgaagcac cgcagtctcg ggctgcgtcg aacggaaatg
     6061 tcgatgcgca aggcgctgtg gatcggattg tcgctgctct tgggtgtacc caaaggggtc
     6121 agtgcagagg aaccaacagg gacagcgacg acggtgacac cggtttcgcc gccagctgtt
     6181 tcgccgccgc ccacggtcgc agccccgcct gcccagcctg cggattctcc gcccgtgccg
     6241 ccagcgatcg aacctgacgg cgaaccgccg ccgcttggct atctgtccga cggaacgcga
     6301 caaaaaccca aagatgcggt gccgtttgcc ggtgtggtgc gcgaaaaagg ctctcgcgat
     6361 ccgctgccgg cgattcgggt gacggtgact ccctggcgag aaaatgcgcc ggccccgtct
     6421 gacaaacccg gcaaaaaagc caaaccacgc ggcgaagcgg ctggcgagcc catcgaagtc
     6481 accaccgacg cggaaggcaa ctttcttgtc gaagacttgg cgccgggcat gtatcaagtc
     6541 cttctgcgcg gcggccaaat tcagcgcacc gagagcatcg agacgctcgc tgccggcaaa
     6601 aaaaccagca tcacctacta cgcaccgcga cggaccaatc agtttgaact ggtggtgcgc
     6661 gccgagccgg tgaaaaaaga agtcagcgaa caagtcctgt cggtgcagga gctgaagcgg
     6721 attcccggca cccaaaatga tgcgatcaag gcggtgcaga acctgccggg tgtggcgcgt
     6781 gcgccgttcg gtgggggtct gctggtcgtc tggggatcgg caccggcgga cactcgcgtc
     6841 tacgccgacg gtgtgcagct gccgcgcatt ttccactttg ccggttttcg cagcaccatc
     6901 aattccgaat ttgtcgacga gctgtcgttc aaacccggcg cctacggtgc ggatttcgga
     6961 cgaggcctcg gcggcatgat cgatgtgacg acgcgctcgc cgaagtccga tcgcatccac
     7021 ggctcggtca cgctcgatct catcgacggc tcggtgactc tcgaaggccc cgtcaccaaa
     7081 aacctgtatg tggccggtgg cgtgcgggtg agctggattt cggtcttttt gccgatcttt
     7141 aaccgctcgc agtttcaggt ttcgccgttt tattgggact atcagctctc gatgcggtat
     7201 cggcccacca aggccgatga catcgacgtg ttcatcttcg gatcgacgga caacctggaa
     7261 gcgcgggtca gcgatccgga cccggcggtg aacatttcgc tcgatacacg gcagtatttt
     7321 tcgcgtgcgc gggtgcgctg gacccaccgt ttttcgtcga gtactcagct tattgtcatg
     7381 ccgtcgattg gcggtgatgt gacgcgcttt gataccggcg atggtggcat cggctcggtg
     7441 tcgttcaagc tcgaaggctt cacgctgggc tataacctgc ggtccgagct gcgccatcgc
     7501 tttgcgccgt ggcttaacgt gattggcggc gtcgactttg aaggctcgcg cagctcgttt
     7561 gaagtcgtgg tgccgccgat tccaccgggc ggtggcggtt ccagcggcga gaccaacaac
     7621 cagaccagcg gatcggcgat tacccagctt ggttcggtgc gcgatcagtc ggtgctgcac
     7681 atcgtgcgga cggcgcccta tgcgattgcg cgctttgact ttctggaccg ccggctgacg
     7741 gtttcgccgc agattcggct ggaaaccgcg tacatccggt cctatgacgg cgatgtctcg
     7801 cgaacggtgt tttcccccga gccgcgcctg gtgataagtg gtcaggtggt gcccaagtac
     7861 cttttgctca agtttggcgt cggggcgtac tcgcagcttg ggcagccgca ggaactgaac
     7921 cgggtgtttg gcaacccgaa cctcgatccg cagcgcggca ccacgtatgt cgcgggcttc
     7981 gagagcgagc cgaccggatc gctgtttgtc caaggccagt ttttctacaa agacctgcgc
     8041 tcgctcatcg tttcggaccc ggtgctgaag tacaccaacg ccggtcttgg gcgggtcatc
     8101 ggcggcgatt ttttgctgcg ccaaaagctg tggaaaggtc tgtttggctg gctcgcgtac
     8161 acgatttctc agtcggagcg aaagagctct ccgcaagacc cgtggcggct gttccgttac
     8221 gatcagacgc acattttgac cgcggtggcg tcgtacaagc tgccgtggtt tggcatcgaa
     8281 gtgggcgttc gtttccgcta tgtgaccggc ggactttaca ccccgacgac tggcgcgatt
     8341 cgcgacaccc tgacgcagac gtgggtcggg acgcagggcg acgtctattc ggcgcggctg
     8401 cccgactttc accagctgga tctgcgcgtc gacaagacct ggacgttcaa tcgctggaag
     8461 ctcgggctgt atctggacat ccagaatctc tataaccgcc aaaacgccga gcagctcgcc
     8521 tatggcggac ggcagctcta ccaaaccacg ctggttactg gtattccgtt tttcccaaac
     8581 tttggtctgc gcgccgattt ctagggaaaa gggcagatga cgacaaaaaa tcgaacacgg
     8641 tttgcgacgg ccttggctct tggtttggcg atgctggctg gctgcgacaa cgacatcgat
     8701 cccgatacgt tggcgcggga cattcgcgtg ctgtcgctgc gcgtcggatc ggcgacaccg
     8761 cttagtccgg cggaagccca agccgaagtc aaacccggcc ccagcggact cgatctggtg
     8821 tttacgagcg accacctgga tttgaccgcc aacgtggctg cgccgacggg accgggacga
     8881 cggatcacgg cgccgcgacc gcttgtctat gagtggtatc tgtgcgtagg tccagcgtcg
     8941 ctgtttagtc cggggacgct cgatccgggc tgtcgcaagt ggcagcccgg tgatcccgat
     9001 ccgatgaaaa gtacgtcgct gcgctacttg ggcagcgggg caaactttag cttgccgacg
     9061 gcggtgctaa aagatgtcgt cggtggcgtg cttcagattt tgctgaccgg cggatcgggt
     9121 ggtggatcgg gcggtacggt gacgattccc gacaagccga tttcgattct tttgccgctc
     9181 atcatgcggg tgcgtgcgca aaacggcgat ccgaatgaca tccgcgacag cgaagtcggc
     9241 gtgacctatc tgcggacctg gatagcgctg cctgggatga cgctgccgcc ggtaaaccaa
     9301 aatcctgcgc tcgcggatct tgtggcgagt gccgacaaag acgggacaaa aaaaccgctg
     9361 ctgccctgca ctgcgacaag ctgtcctcgc aatccggtaa agcggggcgc cgatttgttt
     9421 ttggttggcg catcgctcac tggttccatc gaaacgtaca cgccggtcga cgacactggg
     9481 cgcggtgcaa ggcaagaagc gctgcgctat agctggtttt ccaccgacgg tgcttacgaa
     9541 cgcgagcgaa ccggcgacac ccagcccgac aacacctgga acagcgacaa caagcgtccg
     9601 ccgcccgacg gcatcgtgca gtcctcgctg tggcttgtgg tgcaggacga gcgcggcggc
     9661 ggcgactgtg cccgctacga gctggacctc aactagccgc ctttttgctc cggatgtttc
     9721 ggtttgtcga caaagcctgt caaaaccaag cctgcgcccc cgcgtgagcc gctttttcac
     9781 tacaagccgt cgacgaagac gccacgccca gccaagccgc ggacccgcaa ggccgcctcg
     9841 tcgccaagtg agctgtcgct catctgggga cttttgtacg acctgattcg cgcgccgggt
     9901 tttttgatcc tgctgcttat cggacgggcc gatcccaaag cggcgcttgt gccgtttcac
     9961 cggctgtgga gctttgcgct gtcagcccgc gccaccgcga cgctgattgt gatgaatgtg
    10021 ctggtgtttg ccgtcgagct gtttttgcag ccgcggtggc cctcggcgcg cttttggtcg
    10081 acgtttgctt tttccccgca ggcgctgtgg cagggacagc tcttgcccgc ggttttgcac
    10141 gtcttttccc acgcgagcgt gccgcatctt ttgtcgaaca tgctggcgct gtttgtgttt
    10201 ggccgcgtcg tcgagtcgct gctcgggccg tggcgaatgc ttggcgcgta tgccacggcg
    10261 gcagtcgctg cgacgctggt gtcgcttttg gcgcaaggtc tatcggggca gtatgtgccg
    10321 acgctcgggg cttcgggggc tgtcgctggg ctgattgcac ttggggtgct tttgtcgccg
    10381 ctttcgatca cgtttgaagc gctggttccg atgccactgt ttctggtcgg ctggattgcg
    10441 atggccggcg acgtgctggc gctgtgggcg aaagcgaaag actccgttga tcatccagca
    10501 cacgttggcg ggtatctgtc ggtggtgctg gtgtatcggc tcttgccgca caagctcaaa
    10561 gccaaggcgc gctttggtct tttgctcaac gtgctgacgg tgttgtttgc gcttgtgctc
    10621 tggtacgtcc gcacgcccta aatgccttcg ctccgctgct tgaccgcggt caaagtcagt
    10681 tgatacggtc gcttgtttgg cgaggcagat cattgtgcag cgcaagagca ccctatcgac
    10741 tttacaggta cgacatccgg ccactttgct gattgtggcg gggctgctca cgctgcttgc
    10801 cgttgggtac gcgctgcggt tgcgggtgct gaccggcttt gaatcgcttt tgcccgagtc
    10861 ccgcccgagt gtgctggagc tcaagcgggt ggcgtcgctg acatccggcg tatcgacgat
    10921 gtttatcgtg ctggaaggca acggccagac cccagtcgcc aagctgcgcg ccgccgccga
    10981 tgcgcttgtg ccgaagctgc aagagctggg gccgccgtgg gttggtcatg ccgaaagcgg
    11041 ggtgcaggaa gcgctgcgtt ttctgcgtcc gcgcgccggg atgtttctgc cgctgcccaa
    11101 actacaggaa ctgcacgacg acatcgagga acgcaccgcc aaagcgctgg gggaagcgag
    11161 cggactgttt gtcgacctgg aagacaagcc gagcgagccg ctgacgcggg aaagcctgaa
    11221 aaagcgactg gagctagacc acgagcagac cgaccgttat cccgacgggt actaccaatc
    11281 gcgcgacgga cagacgctgg tggtgctggt acgttccaaa atccacggca gcgactttga
    11341 ccgcggtgcc gaagcgattg ctcgaatttc cgccgtcgtc aaccgggtaa acccggcgtc
    11401 gtttcacccg agcattcgcg tcggctattc cggcgacttg gtgactgcgg tgtcggaata
    11461 ccaggcgata aaccgcgatc tgaccgacgt gggcttgttt gggtcggtgc tcattggcgg
    11521 cgtcgtgttt ttgtattacc tgcgtctgcg caccgtgctg gtcatgctgc tgaccatcgg
    11581 cgttggcgtc gcctggacgt ttggcgccac ccagcttgtc gtcggcagcc tgaatgtcgc
    11641 aaccggcttt ctgtttacca tcatcgccgg caacgggatt aacttcggca tcatttacat
    11701 ggcgcgttac ctcgaggcca ggcgcgtggg tgaagcgctt gagtcggcac tttcgacggc
    11761 ggcgcgagaa acctggatac cgaccctgac ggcgggcttt gcggctgcgg ccagctacgg
    11821 atcactcggg gcgacggatt ttcgcggctt tcatgacttt ggcctgatcg gcggcatggg
    11881 catgctgttg tgctggctcg cgacattcgg ggtgatgccg tgccttttga cggtgtttga
    11941 gcggctgatt ccgctcgatc gtgactttgg cggaccgctg tcgtggctgc gcaagcaaac
    12001 cagaggcggc ggcgtggcgt tttcgcgtcc gtttgagctg ctggtcgcgc gagcgccgcg
    12061 ctttatcacc gccacgggac tgcttctgac cgcgctgggg ctgtttggca ccgtcaagtg
    12121 gctgcgatcc gacccaatgg aatacgattt gcgcaacctg cggaccgatg tgtcgtcgcg
    12181 cgcggatgag atccgtctca acaagctcgg cgatgagatc accgggcacg tcggggcaac
    12241 cgggatggcg attttggtcg accgccccga tcaggtgcaa gagctgaccc aagtgctgct
    12301 ggccaggcgg gccgccgccc ccaaaggtaa ggagccgttt gagagactgc acgcgctgca
    12361 agacttcgtt cccaccgacc aagccgccaa gctgccgctg ctagtgcgca ttcgcaagcg
    12421 gcttgtgcgg gcccacgaac acggcgcaat cagcgatgaa aactggcaag acatcgagcc
    12481 gatgctgccc cccgccgacc ttcagccgtt tggcatcgcc gcgcttcccg aaggactggc
    12541 gcgggcgttt accgaaagcg atggcacccg cggtcgcatt gtttacatcg cgccgacggc
    12601 caccgcgtct gtggatgatg cccactatct gtttcgctgg gccgattctt atcgcgaggc
    12661 ccgcctttcc gacggctcgc tgattcgcgg ttcggggcga gcggtgattt acgccgacat
    12721 gtgggccgcg attgtccacg atgtaccgat tgccgtcgtg ctgtcatttg cggcgacggt
    12781 gctggtcgtg gtcttggcgt ttcgcggcgg gcgttcgtcc tttgcggtgc tcggctcgct
    12841 gtgggccggg gtgttttgga tgatcggcct gctcgtggtg ctcaaggtca agctgaactt
    12901 cctcaacttc attgcgctgc cgattacgtt tggcattggc gtcgactatt ccgtcaacgt
    12961 ggtccagcgc tacgtccgcg aaggccgcgg cagtgcgctt gtcgcaattc gcgaaaccgg
    13021 cggcgcggtc atcctgtgca gcctgaccac catcctgggc tatttggcgc tggttcgttc
    13081 gaccaacttt tcggtacgca gcctcggagt tgcggccttc ctgggcgaaa tctgctgtct
    13141 gctcgcgtcg gtcttgctgc ttccggcggc acttgtctgg atcgatcgtc gtcacaaaga
    13201 cgccgccgct tcccagccgt ccccgccaga cgggccgccc tcaagtggac ctttttacac
    13261 ctaagccgtc aggcgcaaaa acctctccgg tgccatcgtg acctatctgt cgctcgtgct
    13321 gtttgtgctc gccaccgtcg gggtgttgtg tctggcgatc cagaccgggt ttctgtggcg
    13381 ctttctggct gcaaaggtgc gtgttccgac gcagccgctg ccgccgattt cgattctcaa
    13441 gccgctgtcg ggctgtgacg acggactttt ggccaacctg gaatcgtttg cggcgctcga
    13501 ccatcccgat tacgaggtct tgctgggtgt tcacaccatc gacgatccgg cggtttcggt
    13561 ggcccagaag ctgatcgata aacatccaca gcgctttcgc ttggtcgtgc aagacggatc
    13621 gccggggctc aatcccaaag tcaatcagct catcacgttg gcgaaagaag cccgtcacga
    13681 tgtcctgctg gtcagcgacg ccagcgtccg cgtcggaaac ggctatctgc ccgaaatcgc
    13741 cgcctgcctg gaagacccgg acatcgggct cgtcacccac atctttgccg ccgagggaga
    13801 acagtccttg ggctcgctgc tcgacaactt ccacttgatg tcgcactacg gcggcggcgc
    13861 cattgccgcc cgagatgccg gcggacaaac gctggtcaat ggcaaatcca ccgcgtttcg
    13921 ccgtgccgat ctcgatgcga tgggtggttt tgccgcagtc tgtgactatg ccgccgaaga
    13981 ctttgttttg ggattgtggg ttggcaggaa aatcaaaaag aaagtctgct ttgcgcgcta
    14041 tacgccaatt acactatcgg tcaatcggac gctgggtgaa tacattcggc gctatcagcg
    14101 ctggtcgatc atgcagcgca ccggcggcgg ctatctggtg tatctgtcgc agacgatggt
    14161 ctatccgctg attttggcgt cgctgggctt gctgtttcaa ccgtcgctgc gtggtctttt
    14221 ttggtggcta ggaatcggct ttgtcaaagc ggttctcgat tacatccaag ccagcatgct
    14281 gcgttataaa cgaatcagtc cgctgctgat gttgctcggg ccgatcaacg atctgctggt
    14341 tgcctggatt tggacgtatg ggcttttgtg gaacaggacg gtgtggcgag gaacccagct
    14401 caaagtgacg tggggaacca agctcgtgcc gctttcgtaa acccatctac ggcgcgtctt
    14461 tttttgtccc gttctgcttg tcggcaaaca cccgtttcca cagcggata
//
LOCUS       DAORGN010000130        14100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1789_length_14100_cov_5.363831, whole genome shotgun sequence.
ACCESSION   DAORGN010000130 DAORGN010000000
VERSION     DAORGN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_14100_cov_5.363831"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKL17_14150"
     CDS             <1..389
                     /locus_tag="PKL17_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45926.1"
                     /translation="RQAIVYYQRFQQLAPTHRLAPEAKGYVADMRVLLIEQERTVMAR
                     RLLELEQARAKQEAELLRFRAAKAEEEKRVLAEQLRKSSRPVYKRAWFWGVLGSAAAV
                     TATAIGVGVYYAKTPRVDGGFVDIHF"
     gene            399..2288
                     /locus_tag="PKL17_14155"
     CDS             399..2288
                     /locus_tag="PKL17_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK45927.1"
                     /translation="MRNKLLLSGLISLLCSSGCSDTSLVVDVGNIPPIATELRAIVRM
                     SDKQSADLPRFSFPAGRSTYSFGLNLKEKQTGPAQITVATFLNNCLLSSGKVDIPDIA
                     TAGQEVGVNLDAMAADVSADSCPDSKPVLTWVETSQNPATGGIRLTFSGFGFTPTAQV
                     LLDHSTTPASGYVSPNPLEFSVELPRLVPPINHPLHVQVLQPDGGSAVGDFTVSIPVF
                     DGDAPPIFPQSASDPFRLLGPIWAEDLDGDKHVDIIMSSQTTSSDSGYITVYFNDGKG
                     NFTAGNNIPLNADIRDVAAAKLRSGNYRDIVATICHSPTNGNRYSRCSLTVIQQLSAR
                     RFTNTISSNPNLNFGNGDGAENNGQHAFLTVGDFNQDGISDVAAVTNAAQAPYQLTST
                     TGLGSMLKLYDGTAMTKDGFKYSAAKTLPQVAGGIASASFVSASPTQLDLVISEFATN
                     GNGMMEVYPNPGNGRFDQATATQLSTVGRPGKIAAKDFDGDGRTDFAVTLFQNNQMQP
                     GSQVNIYLRRTGSWSAQTISTQLSPFALVGIDLLSDSVPDLIVSNSRSGSQSAQLGFL
                     FNQGAGAFAPMNQTTLPLPSTSDAYLMAADFNEDTKQDLAVSTVGVSGNAGVHVGTLS
                     ILYGL"
     gene            complement(2308..2685)
                     /locus_tag="PKL17_14160"
     CDS             complement(2308..2685)
                     /locus_tag="PKL17_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNK45928.1"
                     /translation="MNVTPNPWLIVGAISGFFSVAGGAFGAHALKQRLPQDLLTVFEV
                     GVRYQMYHALLLCVIGMLPISATLRWAGILCVLGTILFSGSLYVLSLSGIRWLGAITP
                     IGGLCFLAGWIAVAVAAYRLAYS"
     gene            2863..3657
                     /locus_tag="PKL17_14165"
     CDS             2863..3657
                     /locus_tag="PKL17_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNK45929.1"
                     /translation="MNAAAKVNQPIRPYGDRKDDGRIQLSFTLPVAASGRAKEAARRL
                     CEQMGLANIAVSTMEPAGENFSFFVVYASTPQSVDFAEIVVPEVSAPSWSFKELNAII
                     RHHLGRKLVVVGACTGSDAHTVGIDAILNMKGYAGDKGLEAYPWIDAYNLGAQVDNAA
                     LLSRARELSADAILVSQIVTQRDVHRENAKRMIDLARRLGLRDVLLILGGPRIDHKFA
                     RELGFDAGFGPGTKPSQVASYVVQTLLSRRGLEPKEALAEALLHAH"
     gene            3817..9183
                     /locus_tag="PKL17_14170"
     CDS             3817..9183
                     /locus_tag="PKL17_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45930.1"
                     /translation="MPTRSDALPLMTFSSPLLSSVDRLLRGFESRLSLAGASSWLGRM
                     DRFTDRFFASRLSSLGLLAGAPILGFQPRESEPMSLVPAESWIPSPTELRGQTASLNN
                     RLPQAGVVPTARAAAARPSMPIVTPGRTPAIGSDTVAGVAPLTEPIRAGSVTDVAPTA
                     ARISAASAVPGSSPSSAASPLAALATPGVDAASESPTATDPQVRDLVRVAASQQAVRE
                     SLPQATPRVSALGRAFLHLGFSDARLGLSNRDPLQSLFAAPSVDDSMSMVAPDGSRPM
                     AARMAQAQAQSLAQVLVTPDAVPVQKKPAQSVAREAAAQVRESIHRPVGNPSVAESAS
                     AAAAASFVPSPSVALGASHDAAMTEAATAAAPAVAEPAVAAPAVAAPAVAAPASLPSA
                     FGGAEPATPSELSGTTPRAVASPGRSRSARGTSMGTVVPSLWTPMSSALAPELVLGET
                     AAAAYFAHLPTQDAVVLPSLSKPWSAPGSVGARSEQFTSRIGQPTQQIVPSKSAPQSF
                     ATWADAPGVPSQIGQLLAQHATSVARLPRSFDALVQTMDRGLSQSDSPRVVDRFVSPA
                     LPFVPPTESRQKSSEPTSSVSTARQSTAVPATNQTIRQTGPVSAAPGMASGPLSSAPR
                     LSTPAAGLWAPSDEAVSAESDPSVVASSHVRSLVADVPLQREQPSAEPASVFVRQART
                     ATDASAAGPARPWLTPGGMATLAELFAAGVGLSSGGAQRQAESLGLSFVPSMVPSWLP
                     LSASAGERQDLSAGSFVPVRERAAASLPYLPAESLSSSSLSPSAQATSLSSTTKSAPT
                     RRDQPELTVRVGSSPITDRLSSEPAVAGLPALAAMMFGPSASPAREGASPAERGGNLL
                     SQIGALGARSEVFAREHGVVRAEELASSLVSDAGSGFERFSAQVSANRFAPPSVPESV
                     RRQADAQVGRFDATLPALAGTAQRAESRPDGMPISSSSAALPFPGATALRSERLASQF
                     AQSLLGATAGSANLWQGAPGLPSSIAAAVQQSQSVDTPSRWAMGPRGLLFVSEPLPTS
                     LSQTAQTQPKPSQNRSAVAAKAASAVSIPAAGVSGIGSGSDALPQVSATGAAAPVEQA
                     VAPRSVSAADVSHPVAASSRAAALPWQRLGGMGALAELFAASVGVGTGAAASIAQSLG
                     VSAEGSFWPTWLSAISRSTGQPELPARFAAQLPSLTLPSTAEEQASKAQSPSETRRTS
                     PRPLDAFVPAKSSFAPHSAAMLTGGLAATAESFAKQHGIERVAAVSERAMAPSQKAGS
                     GDFAQEESAGRWVSVAGGLVFVPRDASATRDADATNRSLTTKQPTQAAKSTSDVARQS
                     TLSQVPPIGAASSSLSSLSSLSSLSSLSSLDLGRLGSLGLRSELFSALLGPQSAHAGL
                     LGGPQSGWEDIVSMLTSLPKAAQPSDADSSPTMPRWAWSGAGGLLYLGAPQRTSTGGV
                     GPQAARDLASRDAGRPRQAEARGLSSVVAAQPGSLLSSLGSTQQLADRVSGRNQPTER
                     GAEHIASPLLSLVSAPVLDRDDRRGVGDVQRAFGSRLLTGFPRLAQRGDDSDSPTALW
                     PKTMMQQVQRIEKVLSQLPVEWQPSTKVAAAVRQSGIALTPLWQELPRKLNQLKPYET
                     VEPASARDSSSFASTDMAHVAAQRSPALSMVGPTAAPAEARREERGADSNKQQAVEKA
                     MQDAVSSMIKSGGQAAASARLLEAIRAHSLPSPTRTDERMSLGDLSLIALSMGEQRIA
                     ASSPDHPKDRLEPNVSMALRMKKWKHVEDDKNSYRKAVTEHAEQVVKFMKNQMDQAKQ
                     RGQF"
     gene            9424..10557
                     /gene="gcvT"
                     /locus_tag="PKL17_14175"
     CDS             9424..10557
                     /gene="gcvT"
                     /locus_tag="PKL17_14175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNK45931.1"
                     /translation="MSTTNPSAAQPRRTPLYDRHVALGARVIDFGGWAMPVQYESILE
                     EHKTVRTAVGLFDVCHMGEVEFVGPGALAAVERLVSNDVRKLVDGQARYTVVCYEDGG
                     IVDDCIVYRDHAERIVIVINAANIDKDVAWFQKHVEGRSDVVFRNLSDDYGLIAVQGP
                     LAVSLLQRLAPEAELSQVKSFHFCKTDMSGIPVWAARTGYTGEDGFEVFVPAAQTARL
                     WDAMLTVGKDLGVKPCGLGARDTLRLEACLSLYGNDIDATTHPFEANLGWVVKLDHDF
                     IGRSALEAIKAKGLARKLCAFTMEGRGTARHGYGIFDAETGGNQVGIVTSGSVGPTVG
                     KNIGLGYVPLQLAEVGARLFIDCRGKRVAAQVVKSPFYRRPAI"
     gene            10618..11007
                     /gene="gcvH"
                     /locus_tag="PKL17_14180"
     CDS             10618..11007
                     /gene="gcvH"
                     /locus_tag="PKL17_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNK45932.1"
                     /translation="MSKFPTDLRYTKDHEWARIDGKRAVVGITDFAVHQLGDITQVDL
                     PKEGESFRREQVIGTIESVKAVSDIYSPLSGKVVKSNDPLRDQPELLNEDCYDEGWMV
                     ELELSSLDELSQLMTSEEYAKYVKDHS"
     gene            11091..12506
                     /gene="gcvPA"
                     /locus_tag="PKL17_14185"
     CDS             11091..12506
                     /gene="gcvPA"
                     /locus_tag="PKL17_14185"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNK45933.1"
                     /translation="MRYIPHTDADIAAMLRTISAPSIESLFAHIPSSLRLKKPLDIEA
                     LDEAALLSHLGHIGGQNTAALGDALSFLGAGLIPHHVPVAVDMLLQRSEWYTSYTPYQ
                     PEVSQGTLQAVFEYQTLVAELFGPPVVPGQPAPFVSNASMYDGASATAEAVLMARRIT
                     GRQLTIACGAIHPQYLGTLHCYLDGVDEAGKGAIKTVGFGADGRVDLAALGKLLEECG
                     DRVACVLLQSPNYLGVMQDVSSVVALAKKAGALTVVAAAEPLAFGLVKSPGSQGADIV
                     AGEGIGLASGPSLGGPGVGLFAARTEHVRQMPGRLVGETVDQEGRRGYVLTLATREQH
                     IRREKATSNICTNQGLIALAFSIHVCMLGKTGFRRLAEINLAKTEYLKQRLMAVRGFR
                     LAVSGPTFNEFALTVRGRASDAAAKLRERGIFAGVPLDQPGFALPMLPDAERTLLIAA
                     TERHRREDLDRLVAALDEVCP"
     gene            12503..14014
                     /gene="gcvPB"
                     /locus_tag="PKL17_14190"
     CDS             12503..14014
                     /gene="gcvPB"
                     /locus_tag="PKL17_14190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNK45934.1"
                     /translation="MTTTAQPKGLISTKHLLHPSTPIFEQGAPGRSGASLPKLDVPEV
                     AASALLGELHRDIAPALPEVSEVDATRHYTRLSRWNHAIDIGMYPLGSCTMKYNPKVN
                     ERVARMPGFAGLHPAQAESTLQGALELLYRLERSLCEISGFARVTLQPAAGAHGELCG
                     LMMIRAYHQSQGRFPRKVLIPDSAHGTNPASVTLNQMECVPVKTSADGLLSAETVRAA
                     VAQYPGDIAGLMVTNPSTLGIFEEHIAEISAIVHEAGGLVYMDGANFNALLGKVRPGE
                     TGIDVMHINLHKTFTTPHGGGGPGSGPVGVVEKLVPFLPTPTVELRDGVYQLEYNRPQ
                     SVGRLRSFFGNFGMHVRAYTYIREMGGEGLTQATEMAVLNAVYLRALLHEEFPPVSDK
                     PSMHEYVTSDKKVKKETSVQTLDIAKRLMDYGYHPPTVYFPLVVPGALMIEPTETENK
                     ETLDEFAAALLQIAKEAHSEPDLVKQAPHQTALRRLDETKAARHPRLRWLPPT"
BASE COUNT         2575 a         4185 c         4454 g         2886 t
ORIGIN      
        1 cacggcaagc catcgtctac tatcagcggt ttcagcagct cgctccaacc catcggctgg
       61 ctccggaagc caaaggctac gtggcggata tgcgcgtgct tttgatcgaa caggagcgaa
      121 ccgtgatggc acggcgactt ctggaactgg agcaagcgcg cgccaaacag gaagcagagc
      181 ttctgcgctt tcgcgccgcc aaagcggaag aagaaaagcg agtgcttgcc gagcagctcc
      241 gcaagagcag ccgccctgtc tacaagcgtg catggttttg gggggtcttg ggcagtgctg
      301 ccgctgtgac ggcaaccgcg atcggagtcg gagtgtacta cgccaagact cccagagtag
      361 acggaggctt tgtcgacatc catttttagg agaatatgat gcgtaataaa ctcctcttat
      421 ctggcctgat ttctcttctc tgttcctctg gctgttctga tacttcgctg gttgttgatg
      481 tcgggaacat tccgccgatt gcaacagagc ttcgggcgat cgttcggatg tcagacaagc
      541 agtctgctga ccttccccgc ttttcctttc cggctggccg cagcacctat tcgtttggac
      601 tgaatctaaa ggagaagcaa actgggcctg cccaaatcac ggtcgccacg tttctgaaca
      661 actgtctttt gtcatcggga aaagtggaca ttccggacat cgcaacggca ggacaggaag
      721 tcggtgtcaa cttggatgcg atggctgcgg atgtcagcgc cgactcttgt cccgattcca
      781 agccggtgct tacttgggtc gaaacatcgc aaaatcctgc gaccggaggc attcgtctca
      841 ctttttccgg atttggattt actccaactg cgcaggtgct gctggatcat tcgacgacgc
      901 ctgcatcggg ttatgtgtca cccaatccgc tggagttttc tgtcgagctg ccgcggcttg
      961 tgccacccat caaccacccg cttcatgtgc aagtgctcca gcccgatggc ggctctgcag
     1021 tgggtgattt tacggtatcc atcccggtgt ttgacggcga tgcgccaccg atttttccgc
     1081 agagcgcgag cgatccattt cggctgctcg ggccgatttg ggcggaagac ctcgacgggg
     1141 acaagcatgt cgacatcatc atgagctcgc aaactacgag cagcgacagc ggttacatca
     1201 cagtctactt taacgatggc aaaggcaact tcaccgcggg caataacatt ccgctcaatg
     1261 cggacatccg ggacgtcgct gccgccaagc tgcgaagcgg aaattatcgc gacatcgttg
     1321 cgacgatctg tcactcgcca acgaatggaa atcggtacag caggtgtagt ttgacggtga
     1381 ttcagcagct ttcggcgcgc cgttttacaa acacgatctc ttccaatccc aatctaaatt
     1441 ttggaaacgg tgatggtgcg gaaaacaacg gtcagcatgc attcctgacc gtcggagatt
     1501 tcaatcagga tggaatctca gatgtcgccg ctgtcaccaa tgcggcccaa gctccgtatc
     1561 agcttacatc gaccacgggt cttggctcga tgctaaagct ttacgatgga accgccatga
     1621 ccaaagatgg cttcaagtat tccgcagcga agacgttgcc ccaagttgca gggggcatcg
     1681 cgtccgcatc gtttgtgtcg gcttcaccga cgcagcttga cctggtgata tctgagtttg
     1741 cgacaaacgg aaacggcatg atggaagtct atccaaaccc tgggaatggt cgttttgatc
     1801 aagctacggc cacgcagctt tccaccgtgg ggcgaccagg gaaaattgct gcgaaggact
     1861 ttgatggcga tggtcggacg gactttgcgg tcacgctgtt tcagaacaac cagatgcagc
     1921 cgggatcgca agtgaacatc tatctgcggc ggaccggaag ctggtcagca cagacaattt
     1981 cgacgcagct tagtccgttt gcgctggtcg gaatcgatct gttgtcggat tccgttccgg
     2041 atctgatcgt cagcaattca cgcagcggtt cgcagtcggc acagctcggc tttttgttta
     2101 accaaggtgc gggcgctttt gcgccgatga accagaccac gctgccactg ccctcgacaa
     2161 gcgatgcgta tttgatggcc gcggacttca atgaagacac caaacaggat ctggcggtgt
     2221 ccaccgtggg cgtgtccgga aatgccggcg ttcacgttgg tacgctgtcg attctgtatg
     2281 gtctgtagcc gagctctgtg tcgccgttta ggaatatgca agacgatatg cagcgacggc
     2341 gaccgcaatc catcccgcca aaaaacacag acccccgatc ggcgtaatcg caccaagcca
     2401 gcgaatccca gacagcgaaa gtacatacag cgatccggaa aacagaatcg ttccgagtac
     2461 gcagaggatt ccggcccagc gcagcgtcgc actgatcggc aacatcccaa tcacgcacag
     2521 caagagcgcg tgatacattt gatagcgaac gccaacctcg aacacggtca gcagatcctg
     2581 gggtagacgc tgcttgagcg catgtgcacc aaaagcgcca ccagcgacgg aaaaaaagcc
     2641 agagatcgca ccgacaatta accagggatt gggcgttaca ttcatggcac cgcagcatag
     2701 tcgttttgca gacaaactgc caaatcgctt tgctttcaca tattagtcat tttaaattac
     2761 taatattgta gtggtccgtt tcggcggagt gcggtgctgt ggaacgcccg tctgccgcga
     2821 gacgctatct tgcgtggggc gacttctgct aggctcgctc ccatgaatgc cgccgcgaaa
     2881 gtcaatcagc cgattcgccc gtatggagat cgcaaagacg acggacggat ccagctttcg
     2941 tttacgctgc cggtcgccgc ttccggtcgt gcgaaggaag ccgctcggcg actgtgcgaa
     3001 cagatggggc ttgcgaacat cgccgtgtcg acgatggaac cggcgggtga aaactttagc
     3061 tttttcgtgg tctacgccag cacgccgcag agcgtcgatt ttgccgagat cgtcgtcccc
     3121 gaagtgagcg cgccgtcctg gtcgttcaaa gagctaaacg cgatcatccg gcatcacctc
     3181 gggcgaaagt tggtggtggt cggggcttgt accggcagcg acgctcacac cgtcggaatt
     3241 gacgcgattt tgaacatgaa aggatacgcg ggggacaagg ggctcgaagc gtatccgtgg
     3301 attgatgcgt acaatttggg tgcgcaagtt gacaatgcgg cgctgctgtc gcgggctcgt
     3361 gagctgtcgg cagatgcaat cctggtgtcg cagatcgtga cccagcgcga tgtccatcgg
     3421 gaaaatgcca agcggatgat cgatttggcg cgtcggttgg ggttgcgtga tgtgctgctc
     3481 attttgggcg ggccgcggat cgaccacaag tttgcgcggg agcttggctt cgacgcagga
     3541 tttggacctg gtacaaaacc aagccaagtt gcaagctatg tggtgcagac gctgctcagt
     3601 cggcggggac ttgagcccaa ggaagcgctc gcggaagcgc ttttgcacgc gcactaatcc
     3661 cgacagatcc ccgcaggtgc gcacatcggc gccttgcaag attgcaagat tccgacgaga
     3721 gaagacgaca gacgagacga aacacgggct atcatgagcc gataaccagt gtgaataggt
     3781 ctgtctctat cactctgcgt ggaggccatc gctgccatgc ccactcgttc ggatgctctg
     3841 cctcttatga cgttttcgtc accccttttg tcgtcggtgg atcggctact tcgtggtttt
     3901 gaaagccgtc tttcactggc cggagcatcg tcctggcttg gccggatgga tcgcttcacg
     3961 gatcgttttt ttgccagtcg attgtcgtcg ctagggcttt tggcgggggc gcccattttg
     4021 ggttttcagc cgcgcgaaag tgagccgatg tcgcttgtgc ccgcggaaag ctggattccc
     4081 agcccgacgg agctgcgagg acaaactgct tcgttgaaca atcggttgcc acaagctggc
     4141 gttgtcccga cggcacgcgc tgctgcggct cgtccgagca tgcccatcgt gacgccaggc
     4201 cggacccctg cgatcggaag cgataccgtc gcgggcgtgg cacctttgac cgaaccgatc
     4261 agagctggct ccgtgacaga cgtggccccg acggcggctc ggatttctgc tgcgagcgcc
     4321 gtgcctggtt cgtcgccatc ctcggcggca agtccgctgg cagcgctagc cacgccaggc
     4381 gtggatgctg catcggaatc accgacggca acagatcctc aggtccgcga tttggtccgc
     4441 gtcgctgcgt cgcagcaggc cgtgcgcgag tcgttgccac aggcgacgcc gcgtgtctcg
     4501 gcactcgggc gggccttttt gcaccttggc ttttcggatg cccggctggg actgtcgaat
     4561 cgtgatccgc ttcagtcgct gtttgcggcc ccgtctgttg acgactcaat gtcgatggtg
     4621 gctccggatg ggtcaaggcc gatggctgcc cgcatggcac aggcgcaggc gcagagtctg
     4681 gcgcaggtgc tggtgacgcc cgacgccgtc ccggtgcaga aaaagccggc gcagtcggtg
     4741 gcgcgagagg cagccgcgca ggtccgcgag tcaatccacc ggcctgttgg gaatccatcc
     4801 gtcgctgaat cggcatccgc tgccgcggct gcgtcctttg ttccatctcc ctctgtcgcg
     4861 ctgggcgctt cgcatgatgc tgcgatgaca gaagccgcga ccgctgcggc cccggcagtc
     4921 gcggaaccgg cagtcgcggc cccggcagtc gcggccccgg cagtcgcggc cccggcgtcg
     4981 cttccctccg ctttcggtgg ggcggaaccg gcaacaccgt ccgagttgtc tgggacgacg
     5041 ccccgcgctg ttgcttcgcc cggtcggagt cgttccgccc gcggcacgag catgggaacc
     5101 gtggtgccat cgctgtggac cccgatgtca tcggcactgg cgccagagct tgtgctcggt
     5161 gagacggcgg cagccgcgta ttttgcgcat ctgcctaccc aagatgccgt cgtattgccg
     5221 tcgctatcga agccatggag cgcccccgga tctgtcggag cgcgaagtga gcagtttact
     5281 tctcgaattg gccagccgac gcagcagatc gttccgtcga agagcgcgcc acagtcattc
     5341 gcaacctggg ctgatgcccc cggtgttccg tcgcagatcg ggcagcttct tgcgcaacat
     5401 gccacgtcgg tagcgcgctt gccccgctca ttcgatgcgc tggttcagac gatggatcgg
     5461 ggcttgtcgc agtcggactc gccgcgcgtc gtggatcgtt ttgtttcgcc ggcgctgcca
     5521 tttgtacctc cgacggaaag tcgccaaaaa tcgagcgaac cgaccagcag cgtatcgacc
     5581 gcccgtcagt ccactgctgt cccagcgacg aatcagacaa ttcgacagac tgggccggta
     5641 tctgctgcac ctgggatggc gtctgggccg ctttcgtcgg caccgcgtct gagcacgccg
     5701 gctgctgggc tgtgggcacc atccgacgag gctgtctcgg ctgagagcga tccgtccgtc
     5761 gtcgcgtcgt ctcacgtccg gtcgcttgtg gcggatgttc cactccaacg ggagcagcca
     5821 tctgccgaac cggccagtgt ttttgtgcgc caggctcgga cggcgacgga tgcgtcggcg
     5881 gctggaccgg cgcgcccgtg gctgacccct ggcggaatgg caacgctcgc ggagctgttt
     5941 gccgctggcg tcggtctttc gtctggtggc gcacagcgcc aggctgaatc gctgggtctg
     6001 tcattcgttc cgtcgatggt tccgtcgtgg ctgccgcttt cggccagtgc gggggaacga
     6061 caggatcttt ccgccggttc ttttgtgcct gtgcgcgagc gagcggcggc gtcgctgcct
     6121 tatcttccgg cggagtcgct gtcttcttcg tctctgtctc cgtctgcgca ggcgacttcg
     6181 ctttcctcga cgaccaagtc cgctccgacg agacgcgatc agccagagct gaccgttcgc
     6241 gtcggctcat ccccgatcac cgataggctg tcgtcggagc cagcggttgc gggcctgccg
     6301 gcgttggcgg ccatgatgtt tggaccgtcg gcgtcgcccg ctcgcgaagg agcctcgccg
     6361 gctgagcgcg gtggcaacct gctctcgcag attggggcac tcggggctcg cagcgaagtg
     6421 tttgcgcgtg agcatggggt ggtccgtgcc gaagagcttg cttcgtcgct ggtgagcgat
     6481 gcgggttctg gtttcgagcg cttttcggcg caagtttctg cgaaccgctt cgctccgccg
     6541 agcgtaccgg aatctgtccg tcgacaagcc gacgctcaag tggggcgttt cgatgcgacg
     6601 ctgcctgctt tggctgggac ggctcagcgg gcggaatccc gtcctgacgg aatgcccatt
     6661 tcgtcgtcga gcgccgcttt gccgttccct ggcgcgacgg cgctgcgcag tgaacgtctg
     6721 gcgtctcagt ttgcgcagtc gctgctcggt gcgacggcgg gatctgcaaa tctttggcag
     6781 ggtgctcccg gtcttccatc gagcattgcc gcagcagtcc agcagtcgca gtccgtcgac
     6841 accccgtccc gctgggcgat gggtccgcgt ggtctgctgt tcgtcagtga accgcttccg
     6901 acttcgcttt cgcagaccgc gcagacccag ccaaagccgt cgcagaatcg aagcgcggtt
     6961 gcagcgaagg ccgcttctgc ggtgtcgatt cctgccgctg gggtttcggg aatcgggagt
     7021 gggtccgacg cactcccgca agtgtcagcg actggggctg cggcaccggt tgaacaagcc
     7081 gtcgcacctc gttccgtctc agcggcagat gtgtcccatc ctgtcgctgc atcgtcgaga
     7141 gctgcggcgc ttccttggca gcggctcggt ggaatgggcg cactcgcgga actgttcgcg
     7201 gcttccgttg gcgttggaac gggtgctgcg gccagcattg cccagtctct cggtgtgtcg
     7261 gcggaaggtt cgttctggcc gacgtggctg agcgcaattt cgcgttcgac gggccagcca
     7321 gagctgccgg ctcggtttgc tgctcagctt ccgtcattga ctttgccgtc gacagcggaa
     7381 gagcaggcga gcaaggctca atcaccatcg gaaacgcgcc ggacttcgcc tcgcccgctg
     7441 gacgcttttg tgcccgccaa gtcgtcattt gcgccgcact cggcagcgat gttgacaggt
     7501 ggactggcag cgacggctga gtcattcgcc aaacagcacg gcatcgagag agtcgcggct
     7561 gtcagtgaac gcgccatggc accgtcgcag aaggcgggtt ctggcgattt tgcacaggaa
     7621 gagtccgcgg ggcgctgggt ttccgtggct ggtggcctgg tgtttgtgcc gcgtgatgcg
     7681 tctgccacgc gcgatgcaga cgcgacaaac cgatcgctga cgacgaaaca gcccactcag
     7741 gcggccaaat ccacatcgga tgtggctcgt cagagtacgc tttcgcaagt gccgccgatc
     7801 ggcgctgcaa gttcgtcgct gtcatcactg tcgtcgctgt cgtcgctgtc gtcgctgtcg
     7861 tcgctcgact tgggtcgctt gggttctctg gggctgcgca gtgagctgtt ttcggcgctg
     7921 ctcggaccgc agtcggcgca tgcgggcttg ctcgggggac cacagagcgg ttgggaagac
     7981 atcgtctcga tgctgaccag cttgcccaaa gcggcgcagc cgtctgacgc cgattcatcg
     8041 ccgacgatgc cacgctgggc gtggagtggg gctggcggtc tgctctatct gggcgcgccg
     8101 cagcgaactt cgacgggtgg ggtgggaccg caagcggcac gggatctggc ttcgcgtgat
     8161 gcagggcgtc cgcgtcaagc ggaagcacgc gggctctctt cggtcgttgc tgctcagccg
     8221 ggttcgctgt tgtcttctct cggctcgacg cagcaactgg ccgatcgtgt ttcggggagg
     8281 aaccagccga cagagcgcgg cgctgaacac atcgcgtcgc cgcttctgtc gctggtttcg
     8341 gctccggtgc tggatcgcga cgatcgtcgg ggtgtgggcg acgtgcagcg ggcgtttggc
     8401 agtcggctct tgaccggctt cccgcggctt gcccagcgtg gtgacgattc ggattctccg
     8461 acggcgttgt ggccgaaaac gatgatgcag caggttcagc gcatcgaaaa ggttctgtca
     8521 cagcttccgg tcgagtggca accatcgacg aaagttgctg ctgctgtccg tcagagcggg
     8581 attgcgctga cgccgctttg gcaagaactg ccgcgtaagc taaaccagct gaagccgtat
     8641 gagacggtgg aaccagcttc ggcgcgtgat tcgtcgtcgt ttgcgtcgac ggacatggcc
     8701 catgtggcgg cgcagcgatc tccggcgctg tcgatggtgg gaccaacggc agctccggca
     8761 gaagctcggc gcgaggagcg aggcgccgat tcaaacaagc agcaagccgt cgaaaaagcg
     8821 atgcaggatg ctgtctcgtc gatgatcaag tcgggcggtc aagcggcagc gtcggcgcgt
     8881 ctgctcgaag cgattcgggc tcattcgctg ccgtcgccga ctcgcaccga tgaacggatg
     8941 agccttggcg atctatcgct cattgccctg tcgatgggtg agcagcggat tgccgcctcg
     9001 tcgccggatc atcccaaaga tcggctggag cccaacgtgt cgatggcgct gcggatgaag
     9061 aagtggaagc acgtcgaaga cgacaagaac agctaccgaa aagccgtcac tgaacacgcc
     9121 gagcaagtcg tcaagttcat gaaaaaccag atggatcagg ccaagcagcg cggtcagttc
     9181 taactacgcg acggaaacac tgacgacggc aggtttatcc gtcggattcc cttccacatc
     9241 gtcgtcgaaa agcgccgttt tgaagttcac gcgcgcatcc acaaacggtc cgacgggttc
     9301 ggcaaagccc ggtgcgctca gtgtgatttg gggctcgcaa gagggcctgg cgttgggaaa
     9361 cggactgctc cattccgagc aacttgatcc gcaggtgcgg ccctgcgatg atgctagccc
     9421 tccatgagca caaccaatcc ctctgccgcg cagcctcgtc gcacgccact ttatgatcgc
     9481 catgttgctc tcggcgctcg ggtcatcgac ttcggtggct gggccatgcc ggttcagtac
     9541 gagtcgatct tggaagagca caagacggtg cgcactgccg tcggcctttt cgacgtttgc
     9601 cacatgggtg aagtcgagtt tgtcggtccc ggtgcgttgg ccgccgtcga gcgactggta
     9661 tcaaatgacg ttcgcaagct ggtcgacggc caggcccgct acaccgttgt ttgttacgaa
     9721 gacggtggca tcgtcgacga ctgcatcgtc tatcgcgatc acgccgagcg aatcgtcatc
     9781 gtgataaacg ccgccaacat cgacaaagac gtcgcgtggt tccaaaagca cgtcgaaggt
     9841 cgcagcgacg tggtgttccg caacctgtcc gatgactacg gccttatcgc ggtgcaggga
     9901 ccgctcgcgg tttcgctgct tcagcgtctc gcgcccgagg ctgagctaag ccaagtcaag
     9961 tcgtttcatt tctgcaagac cgacatgagc ggcattccgg tctgggcggc gcgcacgggt
    10021 tataccggcg aggacggctt cgaagtgttt gttccggcgg cgcagactgc gcggctatgg
    10081 gatgcgatgc tcaccgtcgg gaaagacctg ggggtgaagc cgtgcgggct gggggcgcgc
    10141 gacacgctgc ggctcgaagc gtgcctgtct ttgtacggca atgacatcga cgcgacgacc
    10201 catccgttcg aggccaacct gggctgggtg gtcaagctcg atcacgactt tatcggtcga
    10261 tcagcgctcg aggcaatcaa agccaagggt cttgcgcgca agctgtgcgc cttcacgatg
    10321 gaaggtcgcg gcaccgcccg gcacggctac ggcatcttcg acgcggaaac cggcggcaac
    10381 caggtcggca tcgtcacttc ggggagcgtc gggccaacgg tgggcaaaaa catcggccta
    10441 gggtatgttc ctctccagct tgctgaagtc ggcgctcggc tgtttatcga ctgtcgtggc
    10501 aaacgggtcg ccgcgcaggt cgtcaagtcg ccgttctacc gtcggccagc gatctaacgt
    10561 gggcttgcgc atgcaggccc cttcgccaac ttccaaaccc aaacgggaca cgttccaatg
    10621 agcaaatttc ctactgatct tcgctacacc aaagaccacg agtgggctcg catcgacggc
    10681 aagcgcgccg ttgtcggcat caccgacttc gcagtgcacc agcttggcga catcacccaa
    10741 gtcgatctgc ccaaagaggg cgagtcgttc cgtcgcgagc aggtgattgg caccatcgag
    10801 tcggtcaagg cggtgtcgga catttattcg ccgctgtcgg gcaaagtcgt aaagagcaac
    10861 gatccgctgc gcgaccagcc ggagctgctc aacgaggact gctacgacga gggctggatg
    10921 gtcgagctgg agctttcaag tctcgacgag ctttcgcagc ttatgacatc cgaagagtac
    10981 gccaagtacg tgaaggacca cagctgagcc actcccttcc gtaaggcgcg tgcgcgttgc
    11041 ccaagtcgca tttttgcact ttggccgacg ctgtgaagcc aaggacatcc atgcgataca
    11101 tccctcatac agacgctgac atcgcagcga tgctgcggac catttccgct ccgtcgatcg
    11161 agtcgctgtt tgcgcacatt ccgtcgtcgc tgcgtctcaa gaaaccgctc gatatcgagg
    11221 cactcgacga agcggcgctg ctaagccacc tgggccacat cggcggtcaa aacaccgcgg
    11281 cgctcggcga tgcgctctcg tttctcggcg ctggtctgat tccgcaccat gtccccgtcg
    11341 cggtcgacat gctgctccag cgctccgagt ggtacacctc gtatacgccg tatcagcccg
    11401 aggtgtcgca aggcacgctg caagcggtgt tcgagtatca gacgctggtg gcggagctgt
    11461 tcggcccgcc ggtcgtgccc ggtcagccgg cgccgtttgt cagcaatgca tcgatgtatg
    11521 acggggcatc agcgacggca gaggcagtcc ttatggcgcg ccgcatcacg ggtcgccagc
    11581 tgacgattgc ttgcggcgcg attcatcccc agtatctggg aacgctgcac tgctatctcg
    11641 atggcgtcga cgaggcaggc aaaggcgcaa tcaagacggt gggctttggt gccgacggtc
    11701 gggtggattt ggctgcgctt ggcaagctcc tcgaagagtg tggggatcgg gtggcctgcg
    11761 tgctgcttca gagcccgaac tatcttgggg tcatgcaaga tgtctcgtcg gtggtggcgc
    11821 tggcaaaaaa agccggcgcg ctgacggtcg ttgcggcagc ggaaccactc gcgtttggtc
    11881 tggtcaagtc gccaggcagc caaggcgcgg acattgttgc gggcgaaggc atcggtctgg
    11941 cttcgggtcc gtcgctgggc ggtccgggtg tgggcctgtt tgctgcgcgc accgaacacg
    12001 tccggcagat gcccggacgc ctggtcggag aaacggtcga tcaagaaggc cggcgtggct
    12061 atgtgctgac gctggcgacg cgcgaacagc acattcgacg tgaaaaagcg acgtcgaaca
    12121 tctgcacgaa ccaaggtctt atcgcgctgg cgttttcgat ccacgtctgc atgctcggca
    12181 agaccgggtt ccgacggctc gccgagatca acctggccaa gaccgagtat ctgaagcagc
    12241 ggcttatggc ggtgcgcggc ttccgtctgg ccgtctcggg cccgacgttc aatgagtttg
    12301 cgttgacggt gcgcggtcgt gccagcgacg cggcggcgaa gctgcgcgag cgtggaatat
    12361 ttgctggtgt tccgctcgat cagccgggct tcgcgctgcc gatgctaccc gacgctgaac
    12421 gcacgctgct gattgctgcg acggaacggc atcggcgcga agacctcgac cgcttggtcg
    12481 cggcgctcga cgaggtgtgc ccatgaccac cactgcccaa cccaaaggtc tgatttcgac
    12541 caaacacctg ctccatccat cgacgccgat ctttgaacaa ggcgcgccgg gtcgttcggg
    12601 tgcttcgctt cccaagctgg atgtgcccga ggtcgcggcc tcggcgctgc ttggggaact
    12661 gcatcgcgac atcgcgccag cgctgcccga agtgtcggaa gtcgatgcga cccggcacta
    12721 cacccggctg tcgcgctgga accacgcaat cgacatcggc atgtacccgc ttgggtcgtg
    12781 cacgatgaaa tacaacccca aagtcaacga gcgcgtcgct cggatgcctg gctttgccgg
    12841 gctgcatccg gcgcaagcgg aatcgaccct gcaaggcgcg ctggagcttc tgtatcgact
    12901 ggagcgctcg ctgtgcgaga tcagcggctt tgctcgggtg acgctgcagc ctgcggctgg
    12961 ggcccacggt gagctgtgcg gtctgatgat gattcgcgcc tatcaccaga gccagggccg
    13021 tttcccgcgc aaagtgctga ttcctgattc ggcgcacgga acgaatccag cgtcggtgac
    13081 gctaaaccag atggagtgcg tgccggtcaa gacgtcggcg gacggacttt tgtcggcgga
    13141 aacggtgcgt gccgcggtgg cgcagtatcc gggcgacatc gccggcctga tggtgaccaa
    13201 tccgtcgacg ctcggtattt ttgaagagca catcgccgag atttccgcca tcgtccacga
    13261 ggctggcggc ctggtttaca tggacggcgc caacttcaac gcgctgctcg gcaaggttcg
    13321 tccgggagag acaggcatcg acgtgatgca catcaacctg cacaagacct tcacgacgcc
    13381 gcacggtggt ggcggtccgg gatcgggtcc ggttggtgtg gtggaaaaac tggtgccatt
    13441 tttgccgacg ccgaccgtcg agctgcgcga tggcgtgtat cagctcgaat acaaccgtcc
    13501 gcagtcggtg ggtcgtctgc gcagcttctt tggcaacttc ggcatgcatg tccgtgccta
    13561 tacgtacatc cgggaaatgg gtggcgaggg gctaacccag gcgacggaga tggcggtgct
    13621 caacgcggtg tatttgcgcg cgcttcttca cgaagagttt ccgccggtgt ccgacaagcc
    13681 gtcgatgcac gagtacgtca cgtcggacaa aaaagtgaag aaagagacgt cggtgcagac
    13741 gctcgacatt gccaagcggc tgatggacta cggctatcat ccaccgacgg tgtacttccc
    13801 gctcgtcgtt cccggcgcgc tgatgatcga accgacggaa accgaaaaca aagaaacgct
    13861 cgacgagttt gcggcggcac tcttgcaaat tgccaaggaa gcccacagcg agcccgatct
    13921 tgtgaagcaa gcaccccatc agactgcgct gcgtcgtctc gacgagacca aagccgcgcg
    13981 tcatccccgt ttgcgctggt tgccaccgac gtagccgtgt agctggtcgc gtaggcccat
    14041 ccctgaggag tctttatgtc tggtcataat cgctggtcca ccatcaagca caaaaaagct
//
LOCUS       DAORGN010000131        14081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1792_length_14081_cov_5.554542, whole genome shotgun sequence.
ACCESSION   DAORGN010000131 DAORGN010000000
VERSION     DAORGN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 14081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14081
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1792_length_14081_cov_5.554542"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..2948)
                     /locus_tag="PKL17_14195"
     CDS             complement(21..2948)
                     /locus_tag="PKL17_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNK45935.1"
                     /translation="MAIFDKLNQFANQFSDLLGDIADDAYAHVELAAQLIERGDYDTA
                     IRELRHALDKRRDHARAHYLLGICYLRRGQAGDLQAAKKALSDALVAREGYVEAHIAL
                     GDVYRKEGDANGAAAEYLQALPFLDDELQRAEVEQLLGSLYLELGQLDKAVRELRKSV
                     SSNPDDAKSQGLLGQALVLQAQKRGETPGSPTWDAARQCLLKAARADTADPQVLTTLG
                     KLLLSSGQVPDAEKALQRAVQAAPERVETLLLFGELRLLQGDTASAYEQGLRAYGSAR
                     QAGNPTMMAEAHRHLARCHAKSGAPERALAALREADAAAPADAGELKKQLLREALDLA
                     LRNGLKTDAVALAARPELADTQIGLVGRAIDENLPIADADALLLRAQAMGDDVEVRLA
                     QAITEQRRGNTAAAASHYRRAASLAPTDPRPRQALAGLYDAERALLPKELYGLLLKVH
                     QHFAKTPELSDWLPDAGKLVETLDRPLLVTVMGEFNSGKSTFVNALLGEEVAPMGITP
                     TTATINILRYGRERAGRVIYRDGQSRTVGWDKVPGLLRGLDPAEASRIRVVEVLYPLE
                     VLARVNVVDTPGLNSILPEHESVAREFIAQADAVIWLFTVDQAGKASELEALHSIRSA
                     GKQILGVLNKIDRLPQMTESADGAPAASVPGSLQAILAHLDDPETGLKDILEVVVPFS
                     GRQALQGRRSKDQALLQKANLPALEQALEERFFQRSQAIKSVAVRTRLSQLLQKAQQR
                     AESLLSQSQRDDLEQALRLLSADALLFARDFIPSERKRLIADAGSAHQVAAQETLSFV
                     RPRRWPFGEHQAAPADRDFLLTLLDEKLSSLCQSSRARVVSALQLAPEDRDLLSLLDE
                     QVYGRYLAFSRGYLRGGKVDDFFVRVLPRLDLSEAAIGRALERDSPTAVDILESELLS
                     PLRSFAERRFQQADHALRDKLAKEELQKLDLDERVLFPLTAFATALTSLPSEPSSPPP
                     HGG"
     gene            complement(2973..3998)
                     /locus_tag="PKL17_14200"
     CDS             complement(2973..3998)
                     /locus_tag="PKL17_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNK45936.1"
                     /translation="MTDPSIRFFSPRTLCRRSGRFLAALSLGSLLLGSTAWAGNVLDR
                     VLAVVNDEIILDTEVLQATEVEAKQALAGVERDSAEGQRRYDELRRRVLDSQIEKLLI
                     AQYAREQKIYVTEDEMRSAIKDVVKNNNLSDESQLREALKAQGLTWDAYSSMLRQQLL
                     QLKVVNTAVRSRVTVGDDEVRSYYAQTVRQVAGDQLQVRIHQIVVPVGKESPAQTVAE
                     RRAKAAKVVEGARAGQDFISLCQKFCDDPSKGDGDTGLVSRSELPVELREVVATMDPN
                     DVRGPIRAERGFYIIRLVEKKDAEVRPFEEVKETLRRQLYEQQVDKAVTSWLKELRRK
                     AHVDVRL"
     gene            complement(3995..5059)
                     /locus_tag="PKL17_14205"
     CDS             complement(3995..5059)
                     /locus_tag="PKL17_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNK45937.1"
                     /translation="MFLPRNGRKRSPRLLLPTLFCTLLATVGCPNLSAKRSTDGKKGT
                     VLAQIDDVSITVSEIEERINSQTPYVRARYTGLEQKKEFLDNLVKFEVLASEARRQGL
                     DRDPEVVRAMKQVMIQKLLKQRFDKLKQEDITDEDVKTYFDSHQDEFNRPPEVRVSMI
                     LVPDEDTAKKVLADPRSKGLENVGFRELVTQYSIDNDTKERGGDLRFFDDKNRELPTE
                     IVSSAFKLANIGDVSEPIKTKHGFAILKLTGQRRALTRTIAEVSQQIRAKLFRERRQR
                     MMDDYERSLRQKSKVEVNDAKLSEVRVDLSKDIGAPEDLRPPKGTGSYHPPFQDAPQA
                     SPLRPGTPTQPPASSAQGTP"
     gene            5248..6093
                     /locus_tag="PKL17_14210"
     CDS             5248..6093
                     /locus_tag="PKL17_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45938.1"
                     /translation="MSKRVSSFAVPFFALLAACGGGTTTGAVIPAGGLNFPNCSDGQL
                     LGIDATGRGYTCVSVATTSAAAPVCTASQALTVEGGMLKCVEKGTGSTTSELSNQITA
                     LEKQYNDLNTKVNDLKTPSGIGSQFVGVTDLKFAGKIVGPKTGVVGVGGAAEACISKF
                     GLGAHMCTPYEMYQSVAAANPAFTSTSTVGKAWVYMIGWNKPTAAQVTGIQATAAADD
                     NAGLGDNCGSYTQGDGTKGWVGTVGSWDVSTYTGAPQAMPHMLRFSISAQTTCDKTFP
                     IACCQ"
     gene            6202..6912
                     /locus_tag="PKL17_14215"
     CDS             6202..6912
                     /locus_tag="PKL17_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45939.1"
                     /translation="MKLPDNCTNGQVLTTDSAGQLVCTSLPVGSQKLPECQPFVEALS
                     SEDGATVKCVARNNENPGGSDAVKAVNQISTTLSTINTTVNNLLGQRQTYGVFKGLTT
                     AKTTGAISSNGKTGILGAADVCATQYGAGAYMCSVYDMYMNVARGKIAQTDTVAKSWV
                     YMTSWKSIATGAANANQGLNDNCASFTNGATNGGYWGTAFEWTALSNGAQGMKFNAGN
                     VTPNSALCSDMLPIACCL"
     gene            6930..7712
                     /locus_tag="PKL17_14220"
     CDS             6930..7712
                     /locus_tag="PKL17_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45940.1"
                     /translation="MSKMKFAFLLSLAPLMMGSDDVASQYEALTVNGMLPICQTGEYL
                     TYQGGNLVCAIPASNGVTGVTNCTGQLLTNTGAGGEFKTLSCTAKGGASISSSDITRV
                     TTLSNNVTQLNTTVNNLTAASPSRGYYVGLTAANTGGQMYVAGSDVGIPSGAKLCSSY
                     GPKAHVCTSYEIYASVAAGKNMATLVDAWVYNESWNFTLSQNPAEPDAGLSENCASFV
                     YSSAHLFYAGQTMTYGALPGGGMGLMLKQRGCPSATKAACCN"
     gene            complement(7825..9843)
                     /locus_tag="PKL17_14225"
     CDS             complement(7825..9843)
                     /locus_tag="PKL17_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNK45941.1"
                     /translation="MARPTEPLRRRLRWLLLATGISAPMNSVAQPILHEPLSVPRLRC
                     KDGVCRPDGASSDAMPDAVMSRDGLVTAPSGGRAPIGQEQIFASGATTENLAHSQGPQ
                     PGTDPPPVRRSRILPDSATGPETGGERLYHEVFAPAVYPYKRMSVLDGVDDDGAMKLR
                     RSDLVAVPVEKTGAAPGRDPFYGSVVVDFVAGQAVPLPTPAAGFRILSYRSTPTRSLS
                     FFVDGAENLYVSSPTGGRHRLVYLMDVAPRYFAGPLLPPGTPAPLLGDVPDDLIAPLP
                     RKLKREASTVLREIGVRTSSGSDYQALLAQLVQYFRGFTVGELPDEGVGDLYLRIALS
                     KRGACRHRSYAFVITALAAGIPARYVENELHVFVEVWIPDARGRGGYFRRINLGGAPL
                     SQRIVDGENRVAYREKGSDPFPTPPNFHSGVPSHVPGQPRQPGSGGSDGSDGTSGTDK
                     SRQSGDSGTGKNRGADGKIAGHSGSEAATVERTAKNGGSDKTKPNSDGDGKSPSGQAS
                     RGASDKNGSSTGDATSPGKPDGSGRKDAAKVPGSSDEASDLPQEDDVSQSDDRRIDQG
                     TTLATTQVSLSASNNAYRGSSLPIRGIVRSTSGNAAGLQVLLILTVSGGPRILGRAVT
                     QPDGRFSLDVDLPSDLPLGNHRLIARVRGDQTRRGSASARYDTLSVGP"
     gene            9929..11827
                     /locus_tag="PKL17_14230"
     CDS             9929..11827
                     /locus_tag="PKL17_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNK45942.1"
                     /translation="MSRRCLPSLPSLRPTLFLASSLLIASSFASCTPCEPIAPLKPDP
                     PPQVTPPPPPKPTGPTEADARTFMNETETQLRALWSYAQRVQFVQNTNITFDTEQLNA
                     QSQEQVMELVGKKIKEAAKFDKLELPPDLRRKFDLLKLAQDLPSPADAGERAELAQLA
                     ASMETTYGKAKYCPPRLKGGCLTLDDLSRTLSTSRNYDELMEAWVGWHDLAKTLRGSY
                     VRYVVLANKGAQNAGYADVGAMWRSRYDMPEDAFAAETERLWEQVKPLYEALHCYTRM
                     KLRQTYGNRVPEAGPIPAHLLGNMWAQEWGNIFPLVAPTGKGAAINLKAKFAQKKTTP
                     IELVKMGEGFFKSVGFDPLPDTFWKRSMFVRPKDRDVVCHASAWDITSKGDLRIKMCI
                     ELDDDNFRTVHHELGHDYYYWYYRDLPLLFQQGANDGFHEAIGDAIALSITPTYLQKI
                     GLMDQGTQVDDTTFLLHDALEHVAFLPFGKVLDQWRWEVFSGKVRPEEYNKRWWELRT
                     KYQGVTPPVNRTDADFDPGAKYHIPSNVPYARYFLARVLEFQFHRALCQAAGYQGPLH
                     KCSIYGNQAAGDKMKAMLQLGSSKPWPEALDAIAGTKSMDGAAMLDYYAPLMNFLKEQ
                     TKSQKCGW"
     gene            complement(11924..12793)
                     /gene="tesB"
                     /locus_tag="PKL17_14235"
     CDS             complement(11924..12793)
                     /gene="tesB"
                     /locus_tag="PKL17_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HNK45943.1"
                     /translation="MSRLLTELVDLLALERIEENLFRGQSQDLGWGQVFGGQVLGQAL
                     SAAVQTVPTERHVHSLHAYFLRLGDAKKPIVYAVDRTRDGSSFSTRSILATQYGKPIF
                     QMIASFQLDEQGFDHADSMQPVLGPEGLRSERDLWAEYSQRLPETIRSRALSERPIEL
                     RPVAPVHPFAPEKSPPEQAVWVRACGTLPDDQALHRYLLAYCSDFAFLPTAMRPHGVT
                     WVTPSMQVASLDHAMWFHRPFRMDDWLLHVMHSPSASGARGLVRGSVFSRDGKLVAST
                     AQEGLVRFRGRIS"
     gene            complement(12876..13349)
                     /locus_tag="PKL17_14240"
     CDS             complement(12876..13349)
                     /locus_tag="PKL17_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNK45944.1"
                     /translation="MRNRDFEALPSALTDHVGYLLVVLGKRAQRAFAAALEADGLRAP
                     HFDIVSFLHERKACSQAEIARALALEPAHLVSLLDELEKSRLVLRSPDPADRRRYSVA
                     LTAYGSVVIKRLFATALAVESDLLGALSQKEQAQLRKLLQRMTRTGDHPSHSPRS"
     gene            13441..13911
                     /locus_tag="PKL17_14245"
     CDS             13441..13911
                     /locus_tag="PKL17_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNK45945.1"
                     /translation="MTPEQTKDSALAVSLSIQDNDWQKLDSLLAPGFTYSGDGLSFSR
                     DEYIGFMQDMKDAFSGMKMTFEHVLVDGEFVTIQFTSRAKNVGKFMGAPASKKDIEVR
                     GTLIRQMRNGIAIQEWQTTDLLGVMTQLGFGALFGYSIAVGLFKAAPKKPVRRV"
BASE COUNT         2654 a         4169 c         4381 g         2877 t
ORIGIN      
        1 ccgcgtggaa acaacctaca tcaaccacca tggggtggcg ggctgctggg ttcgcttggc
       61 agactggtga gcgccgtggc aaatgccgtc agtggaaaca acacccgttc atcaaggtcg
      121 agcttctgca attcttcttt ggccagcttg tcgcgcagtg cgtgatcggc ctgctggaac
      181 cgccgttcgg cgaagctgcg cagcggcgac aacagctcgc tttcgaggat gtcgaccgca
      241 gtgggcgaat ctcgttcgag cgcgcggcca atcgcggctt cactaaggtc gagcctgggc
      301 agcacccgca caaagaagtc atcgactttg ccgccgcgca ggtagccacg cgaaaacgcc
      361 aggtagcgtc catagacttg ctcatcgagg agcgagagca ggtcgcgatc ttcgggcgca
      421 agctggagcg cggacaccac gcgggcccgc gaggactgac aaagagacga cagtttttca
      481 tccagcagcg tgagcagaaa gtcgcggtca gcgggggcgg cctggtgttc accaaagggc
      541 cagcggcgag ggcgcacaaa cgacagcgtt tcttgcgcgg cgacttggtg ggcgctgccg
      601 gcatcggcga taagccgctt tcgctcactt gggatgaagt cgcgcgcaaa cagcagagca
      661 tcggcggaca gcagccgcag cgcttgctct aggtcgtcgc gctggctttg cgaaagcagc
      721 gattcggcgc gctgttgggc tttttgcaaa agctgcgaaa gtcgggtgcg caccgcgacg
      781 ctcttgatcg cctgcgagcg ctgaaaaaat cgttcctcca gggcctgttc gagcgccggc
      841 aggttggctt tttgcagcag cgcttggtct ttgctgcgcc ggccttgcag cgcctgccgt
      901 cctgaaaacg gcaccaccac ctcgaggatg tctttgagcc cggtttccgg atcgtcaaga
      961 tgcgccaaga tggcttgtag tgaaccaggg accgatgcgg caggggcacc gtctgcgctt
     1021 tcggtcatct gcggcaagcg gtcgatcttg tttagcaccc caaggatctg cttgccggcg
     1081 ctgcggatgc tgtgcagtgc ttccagttcg ctcgctttgc cggcctggtc aacggtaaac
     1141 agccagatga cggcgtcggc ttgggcgatg aattcgcggg ccaccgactc atgttcaggc
     1201 aggatggaat tgagtccagg ggtgtcgacg acattgacgc gcgcaaggac ttccagcgga
     1261 tagagcactt cgacgacacg gatgcggctg gcttcggcgg gatcgagccc gcgcaaaagt
     1321 ccgggcactt tgtcccagcc gacggtgcgg ctctggccat cgcggtagat cacccgtccg
     1381 gcacgctcgc ggccatagcg caggatgttg atggtcgccg tcgttggggt gatgcccatc
     1441 ggtgcgactt cttccccgag cagtgcgttt acaaacgtcg acttgccgga gttgaactcg
     1501 cccatcaccg tcaccaaaag tggccgatcg agcgtttcga ccagcttgcc agcatcgggc
     1561 agccaatcgg acagctcggg cgtcttggcg aagtgctgat gcacttttag caaaagaccg
     1621 tagagctcct tgggcaaaag ggcgcgctct gcgtcgtaaa gcccggccaa ggcttgccgt
     1681 ggtcgggggt ccgtcggggc caggctggcg gcgcgacgat agtgactggc tgcggctgcg
     1741 gtgttgcccc tgcgctgctc ggtgatggct tgggccaggc ggacttcgac gtcatcgccc
     1801 atggcttggg cgcgcagtag cagcgcgtcg gcgtccgcga tcggcaggtt ttcatcgatc
     1861 gctctgccga cgagcccaat ctgggtgtct gctagctcag ggcgggcggc cagcgccact
     1921 gcgtcggttt tcagaccatt tcgcagcgcc agatcgagcg cttcccgcaa aagctgcttt
     1981 tttagctcac cggcatcggc gggagctgcg gcatcggctt ctcgcagcgc tgcaagcgct
     2041 cgttcgggcg cgccactttt ggcatgacag cgggccaggt ggcgatgcgc ttcggccatc
     2101 atcgtcggat tgccggcttg gcgagcactg ccataggcgc gcagaccttg ctcgtaagcc
     2161 gacgcggtgt cgccttgcaa aagccgcagc tcaccaaaca gcagcagcgt ctcgacgcgc
     2221 tcgggggcgg cctggaccgc gcgctggagc gctttttccg catcgggcac ttggcccgac
     2281 gaaagcagca gcttgccgag cgtggtcagc acctggggat cggcggtgtc cgcgcgggcg
     2341 gctttgagca gacactgacg agccgcatcc cacgtcggac tgcctggcgt ctccccgcgt
     2401 ttttgtgctt gcagcaccag cgcctgtccg agcagccctt gtgacttggc atcgtcggga
     2461 ttgctcgaaa ccgacttgcg cagctcacgc acggctttgt cgagctggcc gagctccagg
     2521 tacagcgaac cgagcagctg ctcgacttcg gcgcgctgaa gttcatcgtc gagaaacggc
     2581 agcgcttgga ggtactctgc ggcagcgcca ttggcatcgc cttccttgcg gtagacgtcg
     2641 ccgagcgcga tgtgcgcttc gacatagcct tcgcgggcta caagcgcgtc gctgagcgct
     2701 ttctttgcgg cctgcaaatc ccccgcctgt ccccgccgca gatagcagat gccaagcagg
     2761 taatgggcac gcgcgtgatc tcggcgtttg tcgagcgcgt gacgcagctc gcggatcgcc
     2821 gtgtcgtaat cgccgcgttc gataagctgg gccgccagct cgacatgggc gtaagcgtcg
     2881 tcggcgatgt ccccgagcag gtcgctaaac tgattcgcaa actgattgag cttgtcgaaa
     2941 atcgccacag cacacccccg cggcctggct ggttacaatc ttacgtcgac gtgggctttg
     3001 cggcgtagtt ccttgagcca cgacgtgacc gctttgtcga cctgttgctc gtagagctgg
     3061 cggcgcagcg tttccttgac ttcctcgaac gggcggactt cggcgtcttt cttttcgacg
     3121 agccggatga tgtaaaagcc acgctcggcg cgaatcggtc cgcggacatc gtttgggtcc
     3181 atcgtcgcca cgacttcgcg cagctcgacg ggcagttcgc tgcgggaaac caggccggtg
     3241 tcgccgtcac ctttgcttgg atcgtcgcag aatttttggc acaggctgat gaagtcttgg
     3301 ccagcgcggg cgccttcgac gaccttggcg gctttggcgc gacgttcggc cacggtctga
     3361 gccggggact ctttgccgac gggaacgaca atttggtgga tgcggacctg aagctggtcg
     3421 ccggcgactt ggcgcacggt ctgcgcatag taggaccgaa cttcgtcatc accaacggtg
     3481 acgcggctgc gcaccgccgt gttcacgact ttgagctgta gaagctgctg ccggagcatg
     3541 ctgctatagg catcccaggt cagcccttgg gctttcagcg cttcgcgaag ctgtgattcg
     3601 tcgctcaggt tgttgttttt gacgacgtct ttgatcgcgc tgcgcatctc gtcttcggtg
     3661 acgtagattt tttgttcgcg cgcgtactgg gcgatgagca gcttttcgat ctggctgtcg
     3721 agcacgcggc ggcgcagctc gtcgtagcga cgctgacctt cggcggaatc gcgttcgacg
     3781 ccagccagtg cttgcttggc ttcgacctcg gtggcttgca gcacttcggt gtcgaggatg
     3841 atttcgtcgt ttacgaccgc aagaacgcga tccaggacgt tgcccgccca cgccgtcgag
     3901 ccgagcagca agctaccaag cgacagcgcg gccaagaatc gaccgctgcg gcgacaaaga
     3961 gtacgcgggg aaaaaaatcg aatcgacgga tcggtcatgg tgttccttgc gcgctgcttg
     4021 cgggcggctg ggtcggcgtt ccgggtcgca aaggcgatgc ctgcggcgca tcttggaagg
     4081 ggggatgata gctgcctgtg cctttgggcg ggcgcaaatc ttcgggggca ccgatgtctt
     4141 ttgacaaatc gacgcgcact tccgacagct tggcgtcgtt cacttcgacc ttggactttt
     4201 gccgcagcga gcgctcgtag tcatccatca tccgctggcg ccgttcgcga aacagcttgg
     4261 cgcggatctg ctggctgact tcggcgattg tgcgggtcaa tgcgcggcgc tgtccggtga
     4321 gcttcaagat ggcaaagccg tgcttggtct tgatcggttc actgacatcg ccaatgtttg
     4381 ccagcttgaa ggccgacgaa acgatttcag tcgggagttc gcggttcttg tcatcgaaga
     4441 agcgcaaatc tccaccgcgt tccttggtgt cgttgtcgat gctgtattgg gtgaccagct
     4501 cgcggaagcc gacgttttcg agtcccttgc tgcgcgggtc ggcgagcact tttttggcgg
     4561 tgtcttcgtc cggcacaagg atcatgctga cgcgcacttc gggaggccgg ttgaattcgt
     4621 cctgatggga atcgaagtac gtcttgacgt cttcgtcggt gatgtcttcc tgctttagct
     4681 tgtcaaagcg ctgcttgagc agcttttgga tcatcacctg cttcatcgcg cgcacgactt
     4741 cggggtcgcg atcgagccct tggcgtctgg cctcgctggc gagcacttca aacttgacca
     4801 agttgtcgag gaattctttc ttctgctcaa gcccggtgta gcgcgcccgg acgtagggcg
     4861 tctggctgtt gatgcgctct tcaatctccg acaccgtgat acttacgtcg tcgatctgcg
     4921 caagcaccgt gccttttttg ccatcggtcg accgtttggc cgaaaggttc ggacagccga
     4981 ccgttgccag cagagtgcaa aacagcgtcg gcaacagcag ccggggcgag cgtttgcggc
     5041 catttcgagg cagaaacatg ccgaagcaat accgcatccc gtcgggacca cgcaacattt
     5101 ctgggttgag attcgatgtg ctcgatcaaa atcggctttt ttagtgccag ttatgtttct
     5161 gcgtcattcc ggtggcttgt gcgatctgct tgcgccgagg gcaggaacgc gagaaatgta
     5221 atttgtctaa ttttaactgg aggggatatg agcaagcgtg taagtagctt cgctgttcca
     5281 ttcttcgcac tgctcgccgc gtgcggcggc ggtaccacga cgggtgcagt gattcctgcg
     5341 ggcggcctga actttccgaa ctgctcggat ggccaacttc tgggaattga tgcaacgggc
     5401 cgtggttaca cctgcgtgag cgttgcgacg acctcggctg cggccccggt ctgcacggcc
     5461 tcgcaggctc tgacggttga aggtggcatg ctcaagtgtg tcgaaaaggg caccggcagc
     5521 accacttccg agctgagcaa ccaaatcact gcgcttgaga agcagtacaa cgacctgaac
     5581 accaaggtga acgatctcaa gacgccgtcg ggcatcggat cgcagttcgt tggtgtgacc
     5641 gatctcaagt ttgctggcaa aatcgttggc cccaagaccg gcgtagtcgg cgtcggcggc
     5701 gcggcggaag cctgtatcag caagtttggt ctcggcgcgc acatgtgtac gccctacgag
     5761 atgtatcagt cggtcgccgc tgcaaacccg gcgtttacgt cgacctcgac ggttggcaag
     5821 gcatgggtct acatgatcgg ctggaacaag ccgacggcag ctcaggtcac tggcattcaa
     5881 gcgaccgcgg cagcggatga caacgcgggt cttggtgaca actgcggcag ctacacccag
     5941 ggcgatggaa ccaagggctg ggtcggtacg gtgggctcgt gggacgtttc gacgtacacg
     6001 ggtgcgccgc aagcgatgcc tcacatgctg cggttctcga ttagcgcgca gaccacctgc
     6061 gataagacct tccccatcgc gtgctgccag taagcggaaa cggagacacc catgcggact
     6121 agcaataaga aactctacct tgctgcatgt cttgtcgctc cgcttatggc ctgcggaagc
     6181 gagtctaccg gcggcaccca agtgaagctg ccggacaact gcacgaacgg ccaagttctg
     6241 accaccgact ctgctggcca gctcgtctgc accagcctgc cggttggttc gcagaagctg
     6301 cccgagtgcc agcccttcgt tgaggcgctg agctcggaag acggtgcgac ggtcaagtgc
     6361 gtcgcgcgca acaatgaaaa ccccggtggt tccgacgcgg tcaaggcggt aaaccagatc
     6421 tcgaccacgc tgagcacgat caacaccacg gtgaacaatc tgctcggcca gcgccagacc
     6481 tacggcgtgt tcaagggcct gaccacggcc aagacgaccg gtgcgatcag ctcgaacggc
     6541 aagaccggca tcctcggcgc tgccgacgtc tgtgcaacgc agtacggtgc gggcgcttac
     6601 atgtgctcgg tctacgacat gtacatgaac gtcgcgcgcg gcaagatcgc ccagaccgac
     6661 accgtcgcga agtcgtgggt ttacatgacg tcgtggaagt cgattgcgac gggcgcagcc
     6721 aatgccaacc agggcctgaa cgacaactgc gcgagcttca ccaacggtgc caccaacggc
     6781 ggttactggg gcactgcctt cgagtggacc gcactgtcga acggcgcgca gggcatgaag
     6841 ttcaacgccg gcaacgtcac tccgaactcc gcgctgtgca gtgacatgct gccgatcgcg
     6901 tgctgcctat aacgagaagg ggaacgaaca tgagcaaaat gaagtttgct tttctgcttt
     6961 cgctcgcccc gctgatgatg ggcagcgatg acgtcgcctc gcagtacgag gctctgacgg
     7021 tcaacggcat gctcccgatc tgccagacgg gtgagtacct gacgtatcag ggtggcaacc
     7081 tggtgtgcgc gattccggcg agcaacggcg tgactggcgt gaccaactgc accggtcagc
     7141 ttctgaccaa caccggcgcg ggcggcgagt tcaagacgct gtcgtgcacg gccaagggtg
     7201 gcgcctcgat ctccagctcg gacatcaccc gcgtgaccac gctgtcgaac aacgtgacgc
     7261 agctcaacac gacggtgaac aacctgaccg cagcatcgcc aagccgtggc tattacgtcg
     7321 gcctgactgc ggccaatacc ggcggtcaga tgtacgtcgc gggctcggac gtcggcatcc
     7381 catcgggcgc caagctctgc tcgagctacg gcccgaaggc gcacgtctgc acctcgtatg
     7441 aaatctatgc gtcggtcgcg gctggcaaga acatggccac cctggtcgat gcgtgggttt
     7501 acaacgagag ctggaacttc acgctgagcc agaacccggc ggaacccgat gcgggtctgt
     7561 cggaaaactg cgcgagcttc gtctattcgt cggcgcacct gttctacgcc ggccagacga
     7621 tgacttacgg agcgctcccc ggtggcggca tgggtcttat gctcaagcag cgcggctgcc
     7681 ccagcgcaac caaggcggct tgctgcaact agtcgccagc ggtccccttc gggcgtaggc
     7741 gcgaaagggg acgcctgatc tgaaatgaaa aaccccgtct gctgatgtac tggcagacgg
     7801 ggtttttgtt tttccgaagg gattctacgg gccgaccgac agcgtgtcat agcgagcgct
     7861 ggcgctgcca cgacgggtct ggtcgccgcg gacgcgcgca atcagacggt gatttccaag
     7921 cggcagatcg ctcggtagat cgacatccag cgaaaaacga ccatcgggtt gagtcactgc
     7981 gcggcccaaa atgcgcggtc cgccactgac ggtaaggatg agcaacactt gcagtcccgc
     8041 cgcgttgccg ctcgtcgaac gaacgattcc gcgaatgggc aagctgctgc ctcggtaggc
     8101 gttgtttgac gccgacaagg atacctgggt tgtcgccagc gtggttccct ggtcgatgcg
     8161 gcggtcgtcg gactggctga catcgtcttc ttgcggcaga tcgcttgctt cgtcgctgct
     8221 gccggggact ttggctgcgt ctttgcgccc gctgccgtca ggcttgccgg ggcttgttgc
     8281 atcgccggtt gatgaaccgt ttttgtccga tgcaccgcga ctggcttggc ccgacggcga
     8341 cttgccatcg ccgtcgctgt ttggcttggt tttgtcgctg ccgccgtttt tcgccgttct
     8401 ttcgacggtt gcggcttcgc tgccgctgtg accggcgatc ttgccgtctg caccgcggtt
     8461 tttgcccgtg ccgctgtctc cgctctggcg acttttgtcg gtgccgctcg ttccatcact
     8521 cccgtcgctg ccgcctgatc ctggctggcg gggctggccg ggaacgtgag acgggacgcc
     8581 gctgtggaag ttgggcggcg ttggaaacgg atcgctgcct ttttcgcggt aggcgacgcg
     8641 gttttctccg tcgacgatgc gctgtgacag cggggctccg cccaggttga tgcgccggaa
     8701 gtaaccgccg cgaccgcgcg catcgggaat ccacacttcg acgaagacat gcagttcgtt
     8761 ttcgacataa cgcgccggaa tgcccgcagc cagtgccgtg atcacaaatg cgtaggagcg
     8821 atgtcgacag gctcctcgct tcgacagcgc gattcgcagg tacagatcgc caacgccttc
     8881 gtcgggcagt tccccgacgg tgaagccgcg aaaatactgc acaagctgcg ccagcagcgc
     8941 ttgatagtcc gatcccgatg aggttcgcac accgatctcg cgcaggacgg tactggcttc
     9001 gcgcttgagc ttgcgaggca gcggggcgat cagatcgtcg ggcacatcgc cgagcagcgg
     9061 cgctggtgtc cccggcggca gcagcggccc ggcaaaatag cgcggcgcga catccatcaa
     9121 atagaccaag cgatggcggc ccccggtcgg cgacgagaca tagaggtttt ccgctccatc
     9181 gacgaaaaat gacagactgc gggtcggcgt cgatcgatac gacagaatcc gaaaaccggc
     9241 agccggcgtt ggcaacggca cagcttggcc ggcgacaaaa tcgacgacca ccgatccata
     9301 aaatggatct cgacccggtg cggccccggt tttttcgacg ggaactgcca caagatcact
     9361 gcgccgcagc ttcatcgcgc catcatcgtc gacgccatcg agcacgctca tccgcttgta
     9421 gggatagacc gcgggcgcaa acacttcgtg atacagccgc tcgccaccgg tttctggtcc
     9481 cgtcgctgaa tcgggcaaaa tgcgcgacct gcgcacgggc ggcgggtctg tccccggctg
     9541 cggcccctgc gagtgcgcga ggttttccgt cgtcgcaccg ctcgcaaaaa tctgttcctg
     9601 gccgataggc gcgcgtcccc ccgatggcgc agtgacaagt ccgtcccggc tcatcaccgc
     9661 atcgggcatc gcatcgctcg acgcaccatc cgggcgacag actccgtctt tgcagcgcag
     9721 ccgcgggacc gaaagcggtt cgtgcagaat gggctgtgcc acgctgttca tcggtgcgga
     9781 aattcccgtc gccaagagca gccatcgcag ccgtcttcgg agtggttctg tgggcctcgc
     9841 catgcgcctt gccatcatac cttgccggtc ttggcggtgc ccattgcgtg tgttatagcg
     9901 cctccttcgt ttgaggaggt ttcgtctcat gtcccgtcgt tgcttgccgt cattgccgtc
     9961 ccttcggcct accttgtttc tggcttcgtc gctgcttatc gcttcgtcgt ttgcaagctg
    10021 taccccgtgc gagccgattg cgccgctgaa gcccgatccg ccgccgcagg tgacgccgcc
    10081 gccgccgccc aagccgacgg ggccgacgga agccgatgcc cgcacgttca tgaatgaaac
    10141 cgagacgcag cttcgcgccc tgtggagcta cgcgcagcgg gtgcagtttg tccaaaacac
    10201 caacatcacc tttgacaccg agcagctcaa tgcgcagtcg caagagcagg tgatggagct
    10261 ggtcggaaag aagatcaaag aggcggcgaa gtttgacaag ttggagcttc cgcccgatct
    10321 gcggcgcaag tttgatctgc tcaagctcgc gcaggacttg ccgtcgccag ccgatgcggg
    10381 tgagcgggcc gagctggcgc agcttgccgc gtcgatggaa acgacttatg gcaaagcgaa
    10441 gtactgtccg ccgcggctca aagggggctg tctgaccctg gatgatctga gccggacgct
    10501 ttcgacgagc cgcaactacg acgagctgat ggaagcgtgg gtgggctggc atgatctggc
    10561 caagacgctg cgcggttcct atgtccgcta cgttgtgctc gcaaacaagg gcgcacagaa
    10621 cgcgggatat gccgatgtcg gcgcgatgtg gcgttcgcgt tatgacatgc cagaagatgc
    10681 gtttgccgcc gagaccgagc gtctgtggga acaggtcaag ccgctgtatg aagcgctgca
    10741 ctgttacacc cggatgaagc tgcgccagac ctatgggaat cgcgttccgg aggccggtcc
    10801 gattcccgcg catctgctcg gaaacatgtg ggcgcaggaa tgggggaaca ttttcccgct
    10861 ggttgcaccg acggggaagg gcgcagccat caacctaaaa gcgaagtttg cgcagaagaa
    10921 gacgactccc attgagcttg tgaaaatggg cgaaggattc ttcaagtcgg tgggctttga
    10981 tccacttcca gatacattct ggaaacgatc gatgtttgtg cggcccaaag atcgcgatgt
    11041 ggtgtgtcac gcctcggcat gggacatcac gtcgaagggc gatctgcgga tcaagatgtg
    11101 tatcgaacta gacgatgaca acttccgcac cgtgcatcac gagctgggac acgactacta
    11161 ctactggtac taccgggatc ttccgctgct gtttcagcag ggggcaaacg atggtttcca
    11221 tgaagcgatt ggcgacgcga ttgcgctgtc gatcacgccg acgtatctgc aaaaaattgg
    11281 tcttatggat caaggtacgc aggtcgatga caccacattc cttttgcacg atgcgctaga
    11341 gcatgttgcg ttcctgccgt ttgggaaagt gctcgatcag tggcgctggg aagtgttttc
    11401 tggcaaggtc cgtcccgagg aatacaacaa gcgctggtgg gaactgcgga ccaagtacca
    11461 aggggtgact ccgccggtca atcgcaccga tgcggacttc gatccggggg ccaagtatca
    11521 cattccaagc aacgttcctt atgcgcgcta tttcctggcg cgggtgttgg aattccagtt
    11581 ccatcgtgcg ctgtgtcagg cggcgggata tcaggggccg ctgcacaagt gctcgattta
    11641 tgggaatcag gctgcgggcg acaagatgaa agcgatgctt cagcttggtt cgtcgaagcc
    11701 gtggcccgaa gcactggacg cgattgcagg tacaaagagc atggatggtg ccgcgatgct
    11761 cgactactac gcgccgctca tgaacttcct caaagagcag accaagtcgc agaagtgcgg
    11821 ctggtaacgg cctgattcct acccaattcc tgcccgattc ccaatcgatt cctaaccgat
    11881 acctggctga ccccattcgg ggtcttccgc gcgctggctt cgtctaggaa atccgaccgc
    11941 ggaagcggac aagaccctct tgtgccgtcg acgcaaccag cttcccatcg cgtgaaaaca
    12001 cactgccacg gaccagaccg cgcgccccag acgccgatgg actgtgcatg acgtgaagca
    12061 gccagtcatc catgcgaaag ggtctgtgga accacatggc gtgatccaag ctggcgactt
    12121 gcatcgacgg tgtcacccac gtcacaccat gcggacgcat cgccgttgga agaaaagcaa
    12181 agtctgaaca gtacgcgagc agatagcgat gcagcgcttg atcatcggga agtgttccgc
    12241 atgcgcgcac ccacacggct tgctctggcg gcgatttttc cggcgcaaac ggatgtacgg
    12301 gcgcgacagg acgcagctcg attggacgtt cactgagcgc gcgtgagcgg atcgtttcag
    12361 gaagccgctg cgagtattcc gcccacagat cgcgctctga acggagtcct tccggaccga
    12421 gcactggctg catgctatcg gcgtgatcga agccctgttc gtcgagctga aacgacgcga
    12481 tcatctgaaa aatcggcttc ccatactgcg tcgccaagat gctgcgtgtt gaaaagctgc
    12541 tgccgtccct ggttcgatcg actgcataga caatcggttt ttttgcatca cccaggcgca
    12601 aaaagtaagc gtgcagcgaa tgaacatgac gttccgtggg aacggtctgc accgctgcgc
    12661 tcagcgcttg gccaagcacc tgaccgccga acacctgacc ccagccgagg tcttggcttt
    12721 ggccacgaaa aagattttcc tcgattcgtt cgagcgcaag caggtcaacc agttcggtca
    12781 aaagacgaga cataaggact cccagcgaca gtcattttag atgccacgcg ggacttcacc
    12841 cactgaattc gtaagcgtcg ggcaaaagat gtcgcttagc tgcgagggga gtgggatgga
    12901 tggtcccctg tgcgcgtcat ccgctgaagc agcttgcgaa gctgggcttg ctctttttgg
    12961 gaaagcgcac cgagcaagtc ggattccacc gcaagggctg tggcaaacag tcgtttgatg
    13021 acgacgctgc cataggcggt tagcgcgaca gaataccgtc gacggtcggc aggatcaggg
    13081 gatcgcaaaa ccagtcgact cttttccagt tcatcaagca gcgaaaccaa gtgggcaggt
    13141 tccagtgcca gcgcgcgggc gatctctgct tgggaacacg ccttgcgctc gtgcaaaaag
    13201 ctcacgatgt caaagtgcgg tgctcgcagt ccgtccgctt ccagcgccgc cgcaaacgcc
    13261 cgctgcgcac gtttgcccag cacaaccagc agatagccga cgtgatcagt cagcgccgat
    13321 ggcagcgctt caaagtctcg gtttcgcatc gattcaaaac gctagccaga aattgtgatt
    13381 gacacaactg ttttattcgt tgattattgt gccatacaac aattgttagg agcatcgatc
    13441 atgacacccg agcagaccaa ggactccgca ctcgccgttt cgctttcgat ccaggacaat
    13501 gactggcaga agcttgattc tttgctggcc cccggcttca cctattccgg ggatggtctc
    13561 tcgttcagtc gcgacgaata catcggcttt atgcaagaca tgaaggacgc cttttcgggg
    13621 atgaaaatga cgttcgagca cgtcttggtc gacggcgagt ttgtcacgat tcagtttacg
    13681 tcgcgggcca agaacgtcgg caagttcatg ggggctcccg cttccaaaaa agacatcgaa
    13741 gtccggggca ccctgatccg tcagatgcgc aatggcatcg cgattcagga atggcagacc
    13801 accgatttgc tcggcgtgat gacgcagctg ggattcggtg cgctgtttgg ttattcgatt
    13861 gcggtgggac tgttcaaagc ggctcccaaa aaacccgtgc gccgcgtctg atccctcacc
    13921 cttgcgtgga ctgcgcgagc gctgtgttca ttcctgtttc gtcgctgtct gactttagta
    13981 gccgctgtgt ccggtccgtt cctcgatcaa actgatttgt tcttcgagcg tcgccgtttc
    14041 atcggcccag tgcgcatagg ttgggaattc cggaaacgtg c
//
LOCUS       DAORGN010000132        13913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1822_length_13913_cov_5.370111, whole genome shotgun sequence.
ACCESSION   DAORGN010000132 DAORGN010000000
VERSION     DAORGN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13913
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1822_length_13913_cov_5.370111"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKL17_14250"
     CDS             <1..211
                     /locus_tag="PKL17_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45946.1"
                     /translation="TVYPGHGAPEATPAAIDEDIQYLGAAADIFRTAASADAAIAQLK
                     QKFAYTGGGLLDYSTKIYFSTCKQP"
     gene            complement(208..1068)
                     /locus_tag="PKL17_14255"
     CDS             complement(208..1068)
                     /locus_tag="PKL17_14255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK45947.1"
                     /translation="MASDLVGKVFLVTGATEGIGKAAALDFAKRGATLVLVGRNKEKS
                     ERVVADLKRDSGSEKLSLLLGDMSKLDDVRAVARSFAQAHDRLDVLVNNAGALFADYK
                     LSPDGFEMTFALNHLGYFLLTHELLPMLEKTSGSRVVSTSSAAHQTGKMNLDYIAKRD
                     GKSAGFAAYGDSKLANILFTRSLAKRAPGVVVNCFHPGFVQTGFGQNNQGVLASLISF
                     TGKLFARTPEKGAETLIWLATSPDAAKLTGEYFHDKKPAKTTRLAKDDTAAEGLWALS
                     EKLCGITKTS"
     gene            complement(1104..2141)
                     /gene="clpX"
                     /locus_tag="PKL17_14260"
     CDS             complement(1104..2141)
                     /gene="clpX"
                     /locus_tag="PKL17_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNK45948.1"
                     /translation="MARVRPQKRTLLTPRAIYSYLDQHVVGQERAKRVLSVAAYGHLR
                     RCGLPADRKGQIRKSNILLIGPTGSGKTHLVRMLAQVLDVPLSIADATEYTEAGYYGK
                     DVEQLVAELLFRANHSVSDAQRGIVFLDEIDKLARRTQSYKTGGGTRDIGGEGVQQSL
                     LKILEGRELLVPTSVSPQPGRQDVVQVDTSDILFIAAGTFADLFERERPSSRIGFGEK
                     ATASAAKELSTEDLLSYGMLSELLGRLPVRVQLSPLSEDELCQILAGPGDPLLAEYQR
                     LLALDNVQVDFSQDALREIAGAALLQGLGARSLRGLCETVCHDLLFEAPERRGQHIVV
                     DKQYVHARLFP"
     gene            2321..4168
                     /locus_tag="PKL17_14265"
     CDS             2321..4168
                     /locus_tag="PKL17_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNK45949.1"
                     /translation="MSSSHESGVAGETIAEVYANRAQATPNAPAFMTKRNGDWHTLTW
                     QQAYEKSSDIAHGLIAIGAKLGDKIALVGSTREEWTLCDLGLVLAGGITVPIYPSSLK
                     ETVAYILKDSEASFIFVEDKKQLDKLLAIKAEIPQVKQVILWDGHAENDPKVDEWVVP
                     LRELCDRGRAHRLTQPQALPELRKQMTGETVATIIYTSGTTGNPKGVVQSHASFVVGS
                     RYGVNALPVKRSDRQLLFLPLAHSFAKTLSMVAVYVGFCTAFSGIDNILEYVGQVKPT
                     FMAGVPRIYEKVYAGFLNKAKQGGPVKWALVQWAIDVGTKASREIQAGRQPGGLLGIQ
                     WKLADKLVFSKLRDRFGGKLQWFVSGSAPLSRELAEFFHAAGMLILEGYGLTETNSLT
                     SINRVDAYKFGTVGKALHPKLEVKIAADGEILTRGVTNLSGYYKLPEATAEAIEQDGW
                     FHTGDIGEIDSEGFIKITDRKKDLIKTSGGKYVAPQMIENQLKLDPLVSQAVVIGDNR
                     KYITALVAINLDTAKQIIGETGQTPPSDAAALSAHPLVQQRLEDKKKEVNSKLGSWEQ
                     VKYIKVLPRELTEADGELTPSLKVKRKVVTKKYEDLIESMYQAKEKDKD"
     gene            4241..4969
                     /locus_tag="PKL17_14270"
                     /pseudo
     CDS             4241..4969
                     /locus_tag="PKL17_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
     gene            complement(5162..6112)
                     /locus_tag="PKL17_14275"
     CDS             complement(5162..6112)
                     /locus_tag="PKL17_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNK45950.1"
                     /translation="MAAQSQPSPLFASLPARVTVYEVGPRDGLQNEQRMVPTADKIAL
                     INALSQTGLSRIEVSSFVNPKWIPQLADASEVARGITRRPGVEYSCLVPNRRGLDSAL
                     AVGLKEIAVFMSASETHNKKNINKTRADTLLAFGEVMQPALQAGVRVRAYISTVYGCP
                     YEGRVSPDATVELVETLLSMGAYQISLGDTIGVATPRQVEEVLLRLDPLCSRDKLALH
                     FHDTRGTALANVLVGLQMGITTIDSALGGLGGCPYAPGASGNLATEDLIYMLDGMGVE
                     TGINLDALVDCSRQVGTFIGRELPSKYLKAHLAARSRSQV"
     gene            complement(6117..6842)
                     /gene="rnc"
                     /locus_tag="PKL17_14280"
     CDS             complement(6117..6842)
                     /gene="rnc"
                     /locus_tag="PKL17_14280"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNK45951.1"
                     /translation="MSASEQDDLPVLEGYLGHTFSDRSLLCTALRHRSYVNERAPTAD
                     DNERLEFLGDAVLQLAVTHLLMDGDGAALSEGTLTLLRSQLVSEAQLCRLAQQIELGR
                     FLLLGRGEELSGGRQKPSLLADAYEAVFGALYRDGGFVLVLLVAKRLLSETVLRVTES
                     RTLDFKSALQEFLQGQKRARPRYEVIGSEGPDHERVFEVAVWVDAEELGRGHGRSKRE
                     AEHQAAQVALTALRDRTGVLGID"
     gene            6966..8732
                     /locus_tag="PKL17_14285"
     CDS             6966..8732
                     /locus_tag="PKL17_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK45952.1"
                     /translation="MSWPSLGRLLLAVSLCAVPVSQARGDDSTTTAAAVQPPAPSAES
                     TRASAAAEFAKAHQAFESTDYERALRHYSRAYELLPHPSTLYNLALTHERLLDYEAAA
                     QAFAQFLAMPLSEEADAARLQQTHRRLAERSLKRLRDLPARISASAVPDEATVTVQPI
                     GEDGQTGHVMTQIKTPGIFTVPAGRYRLRYSKEGYFPDEVDFDAHIGQALLFSRQLKA
                     KPRSVRLTTSPPAKLFLDDRYLGMTPQLLSVDQGSHRLRIERPFYLTQLRPLELQAGS
                     GTLEFGLQLTPSGRMDMIAGGAIAGAGLGLMVLRLFQGEIENIENMPLREIYKPLVAA
                     ALPAVVGATVAGLAGWEMPVSEAQLLIGSGAWGTVIGFGVGLGAQPQWLLPHVLAVGG
                     GLVGGTVGMAVWRFAKPGGGPVAVWNSAVLWSAHIGALSWAYVITQKPEAAFLGRPPD
                     GRTGEGGFAMLGSTLLGVGLGIGLAHIPQFATLNRRQVAYVDLGGFAGGALFGLVGLG
                     AGYAKTHDLLDSTRIAIPAAMVGIGAGLVTSALLVRRLLPSLQIPTDNGPLIELKSPS
                     LSFVPAGPGGMTVSASLLDGKW"
     gene            8817..10712
                     /gene="mnmG"
                     /locus_tag="PKL17_14290"
     CDS             8817..10712
                     /gene="mnmG"
                     /locus_tag="PKL17_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNK45953.1"
                     /translation="MHYPHEYDVIVVGGGHAGCEAALASARLGMRTLLLSGNLDTIGH
                     MSCNPAVGGVGKGHLVKEIEALGGVMGRAADATGIQFRRLNLSKGAAVRSTRVQSDKM
                     LYRSFVRSFVERMPGLEVQQGDGQKLLYEPVSGHPVERFRVVGVLTTMGIAYRGKAVI
                     LTTGTFLQGKLHVGEQQLEGGRMGEPPARGLSQSLRELGFRIGRLKTGTPCRLDGRTI
                     DWQSLEKQPGDEPLPRFCEDGPAPPLPQLPCYITATTSRTHELILANLHRSPLFAGRI
                     TGVGPRYCPSIEDKVVRFADKPRHQIFLEPEGLSTCEVYPNGISTSLPADIQLQVIAS
                     IPGLERARVLRFGYAVEYDYADPTQLLPTLEAREVAGLYLAGQINGTSGYEEAAAQGL
                     WAGANAALKLRGDGESLVMGRDQAYMGVLVDDLTTRGVDEPYRMMTARAEYRLLLRED
                     NADERLLPLGRKLGLVDDERWQAFTTRQALCEAELALLRQKTVYPDAATNDRLVAIGT
                     TPLSRPSKLAEVLRRPEVSYAGLAQAFSLAQLPPQLGERVETHIKYEGYLDRQAEEAR
                     RFRAMEETPLPPELNYLGIPGLSREIVEKLSKARPASLGQASRIPGVTPAAVSILMVM
                     ARTQRAV"
     gene            complement(10654..12396)
                     /locus_tag="PKL17_14295"
     CDS             complement(10654..12396)
                     /locus_tag="PKL17_14295"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNK45954.1"
                     /translation="MKTRPNLDLLTQLWRRRWLRGLLAVLLLWVVLSKCFFWLTVIRP
                     PRGAGDGFVRPRVVVQDGRTYVGDSWMSKERGILELHLFGEPFSLGYSHGRLGTPYLV
                     QLEDYLFGEMRRYVPSSLALSVIKTGVLYKQRNLLSAVPVPLRFELAGLSDGQPDGQG
                     DFLPPFQRIVFYHALHDITQTIEHSPLLGCTALAATGAATVDGHLYIGRNFDFEGPPM
                     FDSDKAVLFFRPQGRLAFVSVAWTGMAGVVTGLNEEGIYVSVNALRTDDKSDYGLPVE
                     LLLRDVLERAHSLDEAIDIAKSHAVLVPDLYLFADGKTGEAAVIERSPTRFEVVRSRD
                     TIGLANHARTPAFAKDQANERLRTYLTSGARQTRVDELLSRYRGSIDANGVLQILRDK
                     RGTGDKELGLGNRNALDAIIATHSVVVDATERVIWVAKGPHELGEYIGFDLRRELGDG
                     SRPQPASLPEDPTLYSVEYRNFKMMGQSLHAAAVARKHGQWDIAVEEARRAVALSPLS
                     AEAARDLGDLLFERGDQEKQRRSTDWAEARSSYQRFLTLSPPHKIDVEHVDARLKQLA
                     ASAPSPSGSTPPPG"
     gene            12500..>13913
                     /locus_tag="PKL17_14300"
     CDS             12500..>13913
                     /locus_tag="PKL17_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HNK45955.1"
                     /translation="MKQTDVLHEGSPIGKSPPRKEGVAKVTGKAVYVDDLPIAGAIYG
                     VTVRSKVARGHLRGITYHPGVPWEDFHIVTAADIPGENTIALIEKDQVCLVDKLVNHP
                     EEAVVLLAHPDRELCEKARRLVELHIEELPPVFDMDESLEGDVRVWGDDNILKQFSID
                     KGDVARALAEAPIIIEGEYATGAQEQLYIEPQGMVALATPKHGITVWGSLQCPYYVHK
                     ALATLFGVAKERVRIIQCETGGGFGGKEEYPSMLAAHAALLAWKARRPVKMIYDRHED
                     MLATTKRHPSRTRIRSGHSADGKLLALDIDFRLDGGAYMTLSPVVLSRGGLHASGPYH
                     CDHIRVRARAAATNHPPHGAFRGFGAPQSVFAIERHLDQVAAQLGIDPAELRRRNFLA
                     PGQQMATGQVVRDNINLGELLDRAQSLSDYQAKRAEFDRLNAEAEQSGSPIRRGIGLA
                     TFMHGAGFTGSGERYLASVVA"
BASE COUNT         2736 a         4228 c         4472 g         2477 t
ORIGIN      
        1 aacagtgtat ccaggccacg gtgcccccga agcaacccca gcagccatcg acgaagacat
       61 ccagtacctg ggcgcagcag cggacatctt tcggaccgcc gcaagcgccg atgcagccat
      121 cgcccagctc aaacagaagt ttgcctacac aggcggcgga ctgctcgact acagcaccaa
      181 gatttacttt tcgacgtgca aacagcccta gctcgtcttg gtgatgccgc agagcttttc
      241 ggagagcgcc cacaagcctt cggcagcggt gtcatctttg gccaggcgtg tcgtcttggc
      301 cggctttttg tcgtgaaagt attcgccagt cagcttggcc gcgtcggggc tggttgccag
      361 ccagatcagg gtttcagcac ctttttcggg ggtgcgggca aacagcttgc cggtgaagct
      421 gataagcgac gcgaggacgc cctggttgtt ttggccaaag ccggtctgca caaagccagg
      481 gtggaagcag ttgacgacca caccgggcgc gcgctttgcc aaagaccgcg taaacaggat
      541 gttggccagc ttggagtcgc cgtaggcagc aaagcccgcc gacttgccat cgcgcttggc
      601 gatgtagtcc aggttcattt tgcccgtctg atgtgccgcg ctcgacgtgc tgaccacgcg
      661 cgaacccgac gtcttctcta gcatcggcaa aagctcgtgg gtcaaaagga agtaccccag
      721 gtggtttagg gcaaacgtca tctcgaagcc atccggggac agcttgtaat cggcaaacag
      781 cgccccagca ttgttgacca gcacatccag ccggtcgtgt gcctgggcaa acgaccgagc
      841 caccgcccgc acatcatcga gcttggacat gtcgcccagc agaagcgaca gcttttcgct
      901 gccgctgtca cgcttgagat ccgcaacgac gcgctcgctc ttttccttgt tgcgcccaac
      961 caaaaccagc gtcgcgccgc gtttggcgaa gtcgagcgcc gccgctttgc cgatgccttc
     1021 ggttgcccca gtgaccaaaa agaccttgcc aaccagatcg cttgccatac gcccagcctc
     1081 ccaaaaacta tccggacgtc atcttagggg aaaaggcgcg cgtggacgta ctgcttgtcg
     1141 accacgatgt gctggccgcg tcgctcgggc gcttcaaaca gcaggtcgtg acagaccgtt
     1201 tcgcaaagac cccgcaggga acgcgccccg agaccttgca aaagcgccgc acccgcgatt
     1261 tcgcgtagcg catcttggga aaagtcgacc tgaacattgt cgagcgcgag gagccgctga
     1321 tattccgcca agagaggatc gcccggcccg gcaaggatct ggcacagctc gtcttcggac
     1381 agcggcgaaa gctggacccg caccggcagc cgcccgagca gctcgctgag catcccgtaa
     1441 cttagcaagt cttcggtgga tagctctttg gcagccgatg ctgtggcctt ttcaccaaag
     1501 ccgatccgcg acgaaggccg ctcacgctca aacaggtcgg cgaatgtacc ggcggcgatg
     1561 aacaagatgt cgcttgtgtc gacctggacc acgtcttgcc gaccgggctg cggggacacg
     1621 ctggtgggga cgagcagctc gcggccttcg aggatcttga gcaggctctg ctgtacaccc
     1681 tcgccgccga tgtcacgggt gccgccgccg gtcttgtagc tttgggtgcg gcgcgcaagc
     1741 ttgtcgattt cgtccaaaaa gacgatcccg cgctgcgcat ccgatacgga gtggttggcg
     1801 cgaaacaaca gctcggcgac tagctgttcg acatctttgc cgtagtagcc ggcttcggtg
     1861 tactcggtgg catcggcgat gctaagcggc acgtcgagca cctgggcaag catccgcaca
     1921 aggtgggtct tgcccgaacc cgtcggcccg atgagcagga tgttcgactt gcggatctgg
     1981 cctttgcgat ccgcaggcag cccacagcgg cgcagatgcc cgtaggcagc aaccgaaagg
     2041 actcgcttgg cgcgctcttg ccccacgacg tgctggtcga ggtaggagta gatggcccgc
     2101 ggggtcaaca gcgtgcgctt ttgcggacga acacgcgcca tcacggaccc ctcgacaaca
     2161 agccgcagtc gccagatccc gcacgatgca ggctgcgtag gcacggcctg ggcggcatct
     2221 tcttcataaa tggacgcgat tcgcttagcc gtttcaagcg agcgcacgat atcatccacg
     2281 ctaggctttt ttgtaactga actttcccga gggaggaccg atgtcgagta gccacgagag
     2341 cggagtagca ggcgagacga tcgccgaggt gtacgccaac cgggcccagg cgacgccgaa
     2401 tgctccggcg tttatgacca agcgcaatgg ggactggcac acgttgacct ggcagcaggc
     2461 ctatgaaaag tcgtcagaca tcgcgcacgg cctgatcgct atcggggcaa agcttgggga
     2521 caagatcgcg cttgtcggca gcacccgcga ggaatggacg ctgtgcgacc tgggactggt
     2581 gcttgcgggc ggcatcacgg tgccgattta cccgtcgagc ctgaaagaaa ccgtcgccta
     2641 catcctcaag gacagcgaag caagcttcat cttcgtcgaa gacaagaagc agctcgacaa
     2701 gctgctggcc atcaaagccg agatcccgca ggtcaagcag gtgatcttgt gggacgggca
     2761 cgcggaaaac gatcccaaag tcgatgagtg ggtcgtgccg ctgcgcgagc tgtgtgatcg
     2821 gggccgcgcc catcgcctga cccaaccgca agcgctgcca gagctgcgca agcagatgac
     2881 cggcgagacc gtcgcaacga tcatctacac gtcggggacg accggaaatc ccaagggagt
     2941 ggtgcagtct catgcatcgt tcgtcgtcgg ttcgcgctat ggcgtaaacg ccctgcccgt
     3001 caagcgcagc gaccgccagc tgctgtttct gccgctggcc cactcgttcg ccaaaacgct
     3061 gtcgatggtt gccgtctacg tcggcttttg cacggcgttt tcgggcatcg acaacatctt
     3121 ggaatacgtc ggccaggtca aaccgacctt tatggccggt gtgccgcgca tctacgaaaa
     3181 ggtctacgcc ggctttttga acaaagcgaa gcagggcggc ccggtcaagt gggcgctggt
     3241 tcagtgggcg atcgatgtcg gcaccaaggc aagccgcgag atccaagctg gccgacagcc
     3301 cggtggactg ctcgggattc agtggaagct ggccgacaag ctggtgtttt ccaagctgcg
     3361 cgaccgcttc ggcggcaaac tccagtggtt tgtgtcgggc agcgcaccgc tttcgcgtga
     3421 actggcggag tttttccacg ccgccggcat gctgatcctt gaaggctacg ggctgaccga
     3481 gaccaacagc ttgaccagca tcaaccgcgt cgatgcgtac aagttcggca cggtgggcaa
     3541 ggccctgcat ccgaagctgg aagtcaagat cgctgccgat ggcgagatcc tgacccgcgg
     3601 cgtcaccaac ctgtcgggct actacaagct gccggaagcc actgccgaag cgattgaaca
     3661 agacggctgg ttccacaccg gggacatcgg cgagatcgac agcgaaggct ttatcaagat
     3721 caccgaccgc aaaaaggatc tgatcaagac ctcgggtggc aaatacgtcg cgccgcagat
     3781 gatcgaaaat cagctcaagc tggacccgct cgtaagccag gccgtcgtca tcggtgacaa
     3841 ccgcaagtac atcaccgcgc tggtcgccat caacctcgat acggccaagc agatcattgg
     3901 cgagacgggg cagacaccgc ccagcgacgc cgcggcgctt tccgcacacc cacttgtgca
     3961 gcagcgcctc gaggacaaaa agaaggaagt caacagcaag ctcggatcgt gggagcaggt
     4021 caagtacatc aaagtgctgc cgcgcgagct gaccgaggcc gatggtgagc ttacgccgag
     4081 cctgaaggtc aagcgcaagg tcgtcaccaa gaagtacgaa gacctgatcg agtcgatgta
     4141 ccaggccaaa gagaaggaca aagactaacc cggtcgacct gtcgagcggt ccagccacgg
     4201 tcgccacctg gccgtatcga cttgtggcag actgaccggc atgtggattc ctcttttggc
     4261 cctggcggcc ctttgtgcgc tcctgtttgt caccaccagc ttccgcgtgc tccgtgaata
     4321 tgagcgggcg attgtgttcc gacttgggcg ggcacgtcag cgtccgctcg gccccggcct
     4381 ggtgctgatg ctgccgtttg gcatcgaccg cgcccaggtc gtcgatctgc gtaccaaagt
     4441 catccagatt ccgacgcaag aagtcatcac ccgcgacaac atctcgattg gcgtcgatgc
     4501 ggtggtctat gccgatgtgc agagcccagc cgatgcggtg ctgcgggtcg agcagtatat
     4561 gccggcgacg ctccagcttg cggcgacgac actgcgatcg atcatcggtc ggatggagct
     4621 tgacgaggtg ttggccaagc gcgccgaaat caacatcgag gtacagggca tcctcgatgc
     4681 gcgcgtcgag cagtggggca ttgatatcac cgcggtcgag atcaaagaca tctcgctgcc
     4741 agcggaaatg aagcgggcga tggcgcggca ggccgaggca gagcgggagc ggcgcgccaa
     4801 agtgatcatg tcggatggcg agcttcaggc ggcggccaag ctgtcggagg cggcccggct
     4861 gatttctagc gagccggcgg cgctgcagct gcggctttat cagacgatgg tcgaggtttc
     4921 gacgcagccc aacatgacga ttgtgctgcc ggtgccgatc gagcttctgg ccggtcagag
     4981 cgagagcaaa gggggggcac tgacccaagc ggcagtggcc gcagccgcgg cagcgctcaa
     5041 gccgcagctt gaggccggca gcaagggtca agcccctgca cttcccgcca aggcagcgtc
     5101 gacagtcagc gatgaactgg ccgaagcgac agcagccagc aagatcgccg tgacgaaata
     5161 gctagacttg gctgcgagag cgggcggcga ggtgcgcttt gaggtacttc gacggcagtt
     5221 cgcgaccgat gaacgtgcca acctggcgtg aacagtcgac cagcgcatcc aagttgatgc
     5281 ctgtttcgac gcccatccca tccagcatgt agatcaaatc ctcggtggca aggttgccgc
     5341 tcgcgcccgg tgcataggga cagccaccca agccaccaag cgcactgtcg atggtggtga
     5401 tgcccatctg cagaccgacc agcacgttcg caagcgcggt gccgcgggtg tcgtgaaagt
     5461 gcagtgcgag cttatcgcgc gagcacaaag ggtcgagccg cagcagcact tcttcgactt
     5521 gtcgaggggt ggccacgccg atcgtgtcac cgagagatat ctgatacgcc cccatcgaca
     5581 gcagcgtttc gaccagttcc accgtcgcgt cgggactgac cctgccttca tagggacagc
     5641 cgtacaccgt cgagatatac gcccgcacgc gaacgccagc ttgcagcgcc ggctgcatca
     5701 cttcgccaaa cgcgagcagc gtgtcggccc gcgtcttgtt gatgttcttt ttgttgtgcg
     5761 tttcggacgc cgacataaag acggcgattt ccttgagccc gaccgcgagc gcgctgtcga
     5821 gcccacggcg attggggacc agacacgaat actcgacgcc gggtcttcgg gtgatgccgc
     5881 gggcgacctc gctggcatcg gcgagctggg gaatccactt gggattgacg aagctgctca
     5941 cctcgatccg tgacagtccc gtctgagaca gcgcattgat aagggcaatc ttgtccgccg
     6001 taggcaccat ccgctgctcg ttttgcagac catcccgagg gccgacctcg tagacagtaa
     6061 cccgcgctgg cagactcgca aacagtggcg acggttggga ttgcgcagcc atatactcag
     6121 tctatcccca aaaccccggt tcgatcacgt agggcagtta atgcgacttg ggcggcttga
     6181 tgctcggcct cgcgtttgga gcgcccgtgg cctcgtccca gctcctctgc atcgacccag
     6241 accgccactt caaagacgcg ctcgtggtcc gggccttcgc tgccgatgac ttcatagcgg
     6301 ggccgcgccc gtttttgacc ttgcagaaac tcttgcagcg ctgacttaaa gtcgagcgtg
     6361 cggctttccg tcacccgcag cacggtttcc gacaggagcc gcttggccac aagcagcacc
     6421 aagacaaagc cgccatcgcg atagagcgcg ccgaacaccg cttcatacgc atcggcgagc
     6481 agcgaaggct tctgccgacc gccgctcagt tcctcgcctc gcccgagaag gaggaaacgc
     6541 cccagctcga tctgctgggc cagacggcac agctgcgctt cactgacgag ctgcgagcgc
     6601 aagagcgtca gcgtaccttc cgacagcgcg gccccatcgc catccataag caggtgcgtc
     6661 accgcaagct gaagcaccgc atcgcccaaa aattccaggc gttcgttgtc atccgccgtg
     6721 ggcgcgcgtt cgtttacgta gctgcgatgc cgaagggcgg tacacagcag cgaccgatcc
     6781 gaaaacgtgt ggccaagata gccttccagc accggcagat cgtcttgttc ggatgcggac
     6841 acccggcgct tatagcacct gtttgaccgg atgcggaagt cagcttcccc aaattggggc
     6901 gcacctgcta gcgttgcccc cacttcgtcc aagggaaatg ggagtctgtg ggctgcgtgc
     6961 gcgccgtgtc ctggccaagt ttgggtcgtc tgctgctcgc ggtatcgctg tgcgcggtgc
     7021 cggtttcgca agcccgcggc gacgattcga cgacgaccgc agccgccgtg cagccccccg
     7081 ctccaagcgc cgaaagcacg cgggcaagtg ccgccgcaga gtttgccaaa gcgcatcagg
     7141 cattcgagag caccgactac gaacgagcgc tgcgccacta cagccgggcg tatgagctgc
     7201 ttccgcaccc aagcacgctc tacaacttgg cgctcacgca tgagcggctg ctcgactatg
     7261 aagcggcggc ccaagcgttt gcgcagtttt tggcgatgcc gctgtcggaa gaagccgatg
     7321 cggcgcggct ccagcagacc caccggcgac tggccgagcg ctcgctcaag cggctgcgcg
     7381 atctgccggc gcggatctcg gcgtcggcgg tgcctgatga agcgacggtg acggtacagc
     7441 ctatcggcga agacgggcag accggccatg tgatgacgca gatcaagacg ccggggatct
     7501 tcacggtccc ggcaggccgc tatcgactgc gctacagcaa ggaagggtac ttccccgatg
     7561 aagtcgactt cgatgcccac atcggtcaag cgctgctgtt ttcgcgtcag ctaaaagcca
     7621 agccgcggtc ggtacgactg acaaccagcc cgccggccaa gctgtttctg gacgatcgct
     7681 atctgggaat gacgccacag cttctgtcgg tggatcaggg aagccaccgg ctgcgcatcg
     7741 agcgcccgtt ctacctgacc cagctacgcc ccctggagct gcaagcgggc agcggcacgc
     7801 tcgagttcgg gctccagctg acgccaagcg gtcgaatgga catgattgcg ggcggtgcga
     7861 tcgcgggggc ggggcttggc ctgatggtgc tgcggctgtt ccaaggcgag atcgaaaaca
     7921 tcgaaaacat gccgctgcgc gaaatctaca agccgctggt cgccgcagca ctgccggcag
     7981 tggtgggagc gacggtggca gggcttgccg gctgggaaat gccggtcagt gaagcgcagc
     8041 tgctgattgg atcgggggcg tgggggacag tgatcggctt tggcgtgggt ctgggggccc
     8101 agccgcagtg gctgttgccg catgtgttgg cggtgggcgg cggtctggtc ggcggcacag
     8161 tcgggatggc cgtctggcga tttgccaagc caggcggcgg tccggtcgcg gtgtggaaca
     8221 gcgccgtgct gtggagcgca cacatcgggg cgctgtcgtg ggcttacgtg atcacccaga
     8281 agccagaggc ggcgtttctg ggtcgtcccc ccgatggtcg gacgggcgaa ggcggctttg
     8341 cgatgctggg cagtacgctt ctcggcgtcg gactgggaat tggactggcc cacatcccgc
     8401 agtttgcgac gctaaaccgc cggcaagtgg cctatgtcga cctgggtggc tttgccggtg
     8461 gggcgctttt cggtcttgtg gggttgggcg cgggctacgc caagacccac gatctgctcg
     8521 attcaacgcg gatagcgatt ccagcggcga tggtgggcat tggagctggg ctggtcacgt
     8581 cggcgctgct ggtccgcagg ctgcttccgt ctttgcaaat cccaaccgat aacggaccgc
     8641 tcatcgagct gaagtcgccg tcgctaagct ttgtgccagc gggaccgggc gggatgacgg
     8701 tgtcagcctc gctgctggat ggcaagtggt agcaaaaacg agtgatccac cccggtgaaa
     8761 cgggacagcg agtcggttga cacgggcggg cggctttgcc aagttcgccc ccgatgctgc
     8821 actatccgca tgagtacgat gtgattgtgg tgggcggagg tcatgcgggc tgtgaagcgg
     8881 cgctggcgtc ggctcgactg ggaatgcgca cgctgctgct cagcggcaac ctggacacca
     8941 tcggtcacat gtcctgcaat cccgccgtcg gtggagtcgg caaaggccac ttggtcaaag
     9001 agattgaggc gctcggtggt gtcatgggcc gggccgccga tgcgacggga attcagtttc
     9061 gccgcctgaa cctgagcaaa ggggcggcag ttcggtcgac gcgggtgcag agcgacaaga
     9121 tgctgtatcg gtcgtttgtc cgctcgtttg tcgaacgaat gcccggcctg gaagtgcagc
     9181 agggcgacgg ccaaaagctg ctgtacgagc cggtgagcgg acacccggtg gaacgctttc
     9241 gcgtcgttgg cgtgctgacg acgatgggga ttgcctatcg cggcaaagcg gtgattttga
     9301 ccaccggcac gttcctgcaa ggcaagctgc acgtcggcga gcagcagctc gagggcggac
     9361 ggatgggcga accgccagcg cggggcctgt cgcagagcct gcgcgagctg ggctttcgga
     9421 tcggtcggct gaagaccggg acgccgtgtc gactcgatgg acgaacgatt gactggcaga
     9481 gcctggaaaa acagccaggc gatgaaccct tgccgcggtt ttgtgaggac ggccctgccc
     9541 cgccgctgcc gcagcttccg tgctacatca ccgcgacgac gagtcgaacc catgagctga
     9601 tcttggcgaa cctgcaccgc tcgccgctgt ttgcaggccg gatcaccggg gtcggtccgc
     9661 gctactgccc gtcgattgaa gacaaagtgg tgcgctttgc cgacaaaccg cgtcaccaaa
     9721 tcttcttgga accagagggg ctgtccacct gcgaggtcta tccgaacggc atctcgacat
     9781 cactgccggc ggacatccag cttcaggtca tcgccagcat tccagggctt gagcgggcac
     9841 gggtgctgcg ctttggctat gccgtcgagt acgactatgc cgatccgacg cagcttttgc
     9901 cgacgctcga agcacgcgaa gtggctgggc tgtatttggc cggacagata aacggcacgt
     9961 cgggatatga agaggcagca gcgcagggtc tgtgggcggg ggcaaatgcc gcgctgaagc
    10021 tccgtggcga tggggaatcc ttggtcatgg gtcgcgatca agcgtatatg ggggttctcg
    10081 tcgatgactt gacgacacgc ggggtcgatg aaccgtaccg aatgatgacg gcgcgggcgg
    10141 aatatcggct gctcctgcgc gaagacaatg cggatgagcg actgctcccg ctgggtcgca
    10201 agctcgggct tgtcgacgat gagcgctggc aggcgtttac aacccgccaa gcgctgtgcg
    10261 aagccgagct ggcgcttctg cggcaaaaga cggtctatcc cgatgcggcg accaatgatc
    10321 ggctggtggc gattggaacg acgccgctgt cccgcccgag caagctggcc gaggtgctgc
    10381 gtcgtcccga ggtcagctac gccggactgg cccaggcgtt ttcgcttgcc cagctgccgc
    10441 cgcagcttgg ggaacgggtc gaaacccaca tcaaatacga agggtatctg gaccgccaag
    10501 cggaagaagc ccgccgattc cgcgcgatgg aagaaacgcc gctgcctccg gagcttaatt
    10561 acctgggaat tccaggtctt tcgcgtgaaa tcgtcgaaaa actgagcaaa gcgcgaccgg
    10621 cttcgctcgg ccaagcgagc cggattccgg gtgtcacccc ggcggcggtg tcgatcctga
    10681 tggtgatggc gcggacgcag cgagctgttt gagccgcgcg tcgacgtgct cgacatcgat
    10741 cttgtggggc ggcgacaggg tcaaaaaccg ctggtagctc gaccgcgctt cggcccagtc
    10801 ggtggagcgc cgctgctttt cctggtcgcc acgctcgaac aacaggtccc ccaggtcgcg
    10861 cgcagcctct gcggaaagcg gagagagcgc caccgcccgc cgggcttcct cgaccgcgat
    10921 gtcccactgg ccgtgtttgc gcgccaccgc ggcagcatgc agcgactgcc ccatcatctt
    10981 gaagttgcga tactcgacgc tgtagagcgt ggggtcttcg ggaagtgaag caggttgggg
    11041 acggctgcca tcgcccagct cgcgacgcaa atcgaagccg atgtattcgc ccagttcgtg
    11101 cggtcctttg gcgacccaga tgacccgctc ggtcgcatcg acgacgaccg aatgggtggc
    11161 aatgatcgca tcgagagcat tgcggttgcc aagacccagc tctttgtcgc ccgtgccgcg
    11221 cttgtcacgc aggatctgaa gcacgccatt tgcatcgatg ctgccgcgat agcgcgaaag
    11281 cagctcatca acccgagtct gccgcgcccc cgacgtcaga taggtgcgca ggcgctcgtt
    11341 ggcttggtct ttggcaaacg cgggcgtccg ggcgtggttg gcaagcccga tcgtgtcccg
    11401 gctgcgcacc acttcaaaac gagtgggcga gcgctcaatc accgcagctt ccccggtttt
    11461 cccatctgca aacaggtaca gatcggggac caagaccgca tgcgatttgg caatgtcgat
    11521 ggcttcatcc agggaatgcg cacgctctaa gacatcgcgc agcaaaagct cgacgggcag
    11581 cccgtaatcg ctcttgtcat cggtgcgcag cgcattgacc gacacataaa taccttcttc
    11641 gttaagacct gtgaccaccc cggccatgcc ggtccacgcc accgatacga atgccagccg
    11701 gccctgcggc ctgaaaaaca gcaccgcttt atcactgtcg aacatcggcg gcccctcgaa
    11761 gtcgaagttg cgcccgatgt ataggtggcc atcgaccgtc gccgcccccg tcgctgccag
    11821 tgccgtacaa cccaaaagcg gcgagtgctc tatcgtctgg gtgatgtcgt ggagtgcgtg
    11881 atagaagacg atgcgctgaa agggtggcag aaagtcgcct tggccatcag gttgtccgtc
    11941 ggaaagtccc gcaagctcaa agcgcagcgg cacaggcacc gcggacagca ggttgcgctg
    12001 cttgtaaagc acgccggtct tgatcacaga cagcgccagc gaggacggga cgtagcggcg
    12061 catctcaccg aacagatagt cttccagctg gaccagatac ggagtcccga gcctgccgtg
    12121 actgtagccg agcgagaacg gctcaccaaa aagatgcagc tccaagatgc cgcgctcttt
    12181 gctcatccag ctatcgccga cgtaggtgcg cccgtcttgg accacgactc ggggccggac
    12241 aaagccgtcg cctgccccgc gcggaggccg aatcaccgtc agccaaaaga agcatttgga
    12301 cagcaccacc caaagcaaga ggaccgccaa aagtccccgc agccagcgac ggcgccaaag
    12361 ctgagtcaaa agatccaggt tcgggcgcgt tttcataagc cgacgtatct aacacgtccc
    12421 gtgcgatctc gaacgaaggg aagtgcgagt tgtgcagccc tgttgacaca gggtggggtg
    12481 atttccgaca gttggcggcg tgaagcagac tgacgttctc cacgaaggtt ctccgatcgg
    12541 caagagcccg ccgcgcaaag aaggcgtcgc caaagtcacc ggcaaagcgg tgtacgtcga
    12601 cgatttaccg attgccggcg cgatctatgg cgtcacggtg cgcagcaaag tcgcgcgcgg
    12661 ccacctgcgc ggaatcacct atcaccccgg tgtgccgtgg gaagacttcc acattgtcac
    12721 cgccgcggac atcccagggg aaaacaccat cgcgctgatc gaaaaagatc aggtgtgcct
    12781 ggtcgacaag ctggtaaacc atcccgaaga agcggtggtg ctgctggcgc atcccgaccg
    12841 cgagctttgt gaaaaagcgc ggcggctggt cgagctgcac attgaggagc tgccgccggt
    12901 gtttgacatg gacgaatcgc ttgagggcga tgtccgggtg tggggtgacg acaacatcct
    12961 caagcagttt tcgatcgaca aaggcgatgt cgcccgcgcg ctcgccgaag ccccgatcat
    13021 catcgagggt gaatacgcga ccggcgcgca agagcagctc tacatcgagc cgcaggggat
    13081 ggtggcgctg gcgacgccca agcatggcat tacggtgtgg ggctcgctcc agtgtccgta
    13141 ctatgtccac aaagcgctgg cgacgctgtt tggggtggcc aaagaacggg tgcggatcat
    13201 ccagtgcgag acgggcggcg gtttcggcgg caaggaagaa tacccgtcga tgctggcggc
    13261 ccatgcagcg cttttggcgt ggaaggcgcg gcgtccggtc aagatgatct acgaccgtca
    13321 cgaagacatg ctagcgacga ccaagcggca tccgtcgcgg acgcggattc ggtcgggaca
    13381 cagcgccgac ggcaagctac tcgcgctgga tatcgacttc cggctcgacg gtggcgctta
    13441 catgacgctg tcgccggtgg tgctgtcgcg tggcggactg catgcctcgg ggccttatca
    13501 ctgtgatcac atccgggtgc gggcgcgcgc ggcagcgacg aaccatccgc cgcatggagc
    13561 gtttcgcggc tttggtgcgc cgcagagtgt gttcgcgatc gagcgacacc tggatcaggt
    13621 ggcagcacag cttggcatcg atccggcgga gctgaggcgg cgtaactttt tggcaccggg
    13681 ccagcagatg gcgacgggtc aggtggtgcg cgacaacatc aacctcggcg agcttttgga
    13741 tcgggcgcag tcgctgtcgg actatcaggc aaagcgggct gagttcgacc gtctgaacgc
    13801 agaggccgaa caaagcggat caccgattcg cagaggcatt ggtctggcca ccttcatgca
    13861 cggcgccggt tttactggtt cgggcgagcg ttacttggcg tcggtagtgg ccg
//
LOCUS       DAORGN010000133        13748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1846_length_13748_cov_5.534580, whole genome shotgun sequence.
ACCESSION   DAORGN010000133 DAORGN010000000
VERSION     DAORGN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1846_length_13748_cov_5.534580"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PKL17_14305"
     CDS             complement(<1..1015)
                     /locus_tag="PKL17_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45956.1"
                     /translation="MTQVTRQILVLSLGMLLPSWALAQTTGSTTTTTSSTAGNGSCSG
                     TVNGKTISGRTMLSLGGVPVELNTGPAGPQSVGCGASSDSIDIVQQNIGTAFGKAECQ
                     CHGRGLKMRVILDSPVASDGQPFNSSMYVGQDNCSDQTQRTTTGAQCEQISSTNPPEN
                     TVFSIKPESFRVAGSPVDIVLPAEALTKPRPVGTSPTGYEYVCDTGGPQNVTAQVLLG
                     PDSSPATCKLPLVVNTAGPTAPKIESVGSGNGGLMVYWSVPESTSGILFYQLLCRNKK
                     DPNTPVMSDDFLANTRHYFSACIDGVLYRRPYNADNTNTTEPHPGRGDKPAAGTFLVD
                     PHFI"
     gene            complement(1019..2491)
                     /locus_tag="PKL17_14310"
     CDS             complement(1019..2491)
                     /locus_tag="PKL17_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK45957.1"
                     /translation="MASLRRQRERAGLSRAELAHKCGLSESTIRNLETGRHVPCAQTL
                     QAIATVLNPLGPANPPIGPSLAVTPQLGFAPARGPGVWLGSDDMLHLYERLRRALAGE
                     GGYLPLPWLLSDPACATVFSDFRNRHDPLVQRGLWKDVAQVVRQLVGRRTLDVLALAC
                     ADARDEISLLGWLLSDGLLRMRVLLMEQSSALLGAALRRAVMQLGDVRSIQLAGLQAA
                     LTELPQHAMWSGDHPRLFTLLAPQFGLLDGEMLQLRQTLWLARPLDLLLLGFDVAAGP
                     AQDRRYLQQRDPMLADARRAEPQLVRLAETLFRNHVQAPCDLRLSTAIDLASCAIPSS
                     YAVELRAEVQGQSSRPRQFSVWRSKRYQPSALAQSMQQEGFRLLAEWQQHEQTPAMGL
                     QLYQRTVSSVVCAAEARPPWGSGNVLGIGESLPSPQTLAPSLDTFCTGVVLRRGRRRG
                     VTLCRAIEQARCQPVPANRRGLAHSVGERAAKLARLLLRR"
     gene            2869..3534
                     /gene="tmk"
                     /locus_tag="PKL17_14315"
     CDS             2869..3534
                     /gene="tmk"
                     /locus_tag="PKL17_14315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNK45958.1"
                     /translation="MVTKKTAGRGFFLVFEGIDGAGTTSQQRWLCEELERQGMQVHKT
                     AEPTANPVGRLIREVLRGEHAPFSAHALALLFAADRADHLSREIDPAVLAGKVVVSDR
                     YVLSSLAYQTMAGVPDDLVAQANAPFRTPDLTVYFDLPVEVAAQRRAKRGQAQEIFEV
                     DDFQRGVATRYLDHAKRWQREGKPIVFVDAAQSFDAVSRQLYDLVLPHLVGTGDQRSG
                     TID"
     gene            complement(3510..4037)
                     /locus_tag="PKL17_14320"
     CDS             complement(3510..4037)
                     /locus_tag="PKL17_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45959.1"
                     /translation="MKTRTADLLAVALLSVGLGAGCSDSSDACEGRTETCLSLTLDGA
                     DGVASADQLKVLIWRKDQPLSPTTPLGARQDFPFKVALLWPDGPASVSVRSLLGGQVN
                     GVSAEIALDLRNGQHAQRKLTLFPPLPVTPPLDMAKPRDLSMVPDMAMPLPDLAMPLP
                     DLAMPPLDQSMVPDL"
     gene            complement(4040..4954)
                     /locus_tag="PKL17_14325"
     CDS             complement(4040..4954)
                     /locus_tag="PKL17_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45960.1"
                     /translation="MKRRLTVFACGAFLFTASSAFAQAKAPAPTDAKAESERAGKEGE
                     RLLAAHDAAGAVTELGKAYSLSKNPRFLLPLGLAFAEVGRPLDALDALGRFLKESPTL
                     PDQKRREIGAKLGVMLDQVAGTVNIEASRQNAQIKLDGRVIGMSPIETPLRLNPGKHE
                     LQMVPAPTDPSSGATVVIDIKPGEQKAIKLEPSARSKFLEPQVSNQDADPALRTTAAN
                     QQSVSTQAEPATKGSEFNFRDLPKKWWFWTGVGAVAAVGIGLGVGLGVGLRDRSTPPT
                     ESFPAVTTWPGGPIDVRMPSVVAQTFGY"
     gene            5143..6792
                     /locus_tag="PKL17_14330"
     CDS             5143..6792
                     /locus_tag="PKL17_14330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK45961.1"
                     /translation="MSTENEWAASMIGRTLDGQYRLDGILGEGAMGVVFRATNLAVDK
                     AVAIKMMRKETFAEPDAVERFKREAKLWSQLNHPSIAQVFDFGLSDDMPYLVMELIEG
                     DQLSDVLDREGPLPPLRAISVMRQLAAALEEAHRHGVVHRDIKPQNLMLLRYKTGGRL
                     TLKVLDFGMAKQVGSKAMTLTAPGILVGTPKYVAPEQVTENPKIDGRTDLYAAGVLFY
                     EILTGQPPFVGTPHDVLFAHLGRQPEPLPDSIPQCVTDVVMKLLRKKPAERYQNAVEL
                     EHALETCEDALRGPSISSASNPVLTESTVRRAIETRRGHRSGKRKLGEAGPRYGLWGL
                     LGALGMAAFGLLALSSSLSLQRKLASVMSVYKVPQDETVQNELVRLQSEAQLRAWPVV
                     LQGTEDLQRTYLSSLLPSQSSQVQALRQKALLEQPLQPIYERFQAAVERKDVEEAIRL
                     YAQFSNDSVYRTLAEPVFNQLSEAFVSQHLQRASSLRSEHRCPEFQAEVQKILDAMPG
                     HPLARAEQRKECVPQAIEPPQQPGTAPVSTPTPALPAPSKQ"
     gene            complement(6892..8718)
                     /locus_tag="PKL17_14335"
     CDS             complement(6892..8718)
                     /locus_tag="PKL17_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45962.1"
                     /translation="MKQTSSAKMGRSARWLLAGVLVAGSGQFAHAGPQDRIANMNKQA
                     MDDYDRLEFNAAKKTLEDTVALLRKHALEGTIQAARTYINLGMVYVQLKDSVRGEQWF
                     RRALSINPNAKLDPNLATPEVKTVWAIATGTAQAPMPQPVQPVQQPIAQPVQQPIAQP
                     VQPAQPTQSDPLLDVETKRPEPPKQPLEYSADDLLDPVKKCELRHTPLSETRTKTKVN
                     LYITPTSIPIARASIFYRSPGQERFTESPMIPSRKQPGDLVGVIPPDALLGKSIQYYI
                     EGYDAKGRPCGTQGVAENPIIMTVSPYAGIVVAQSEDVEDPMKYVKQEAERQRLAGLK
                     DRVYFDLGIGTGGALITSGTTTEVAWFYNPKTTKYEQAKSSSTGFTWSGLGVRAEFGA
                     FLWRGLSLGVSGRFEAMLNHNADSSDSAMKNNLCTDSQGRPSPCFATTAKGQFGYLVL
                     GKLRYQFSPGRIFRPYIHADIGGGEWRGGLNIDGSRPMKNGAIDTDSPLQPTDQCSAE
                     YNGKTTDAERKPAGCTSVGGNIGYNQQTGTPSAQYLNRVCPTGGSCIDSVVLGKLFVG
                     AGAGFYVGGRHAGVSLDVNLLTSLSGNTGLLIDAYAGPQFLF"
     gene            complement(9044..10252)
                     /locus_tag="PKL17_14340"
     CDS             complement(9044..10252)
                     /locus_tag="PKL17_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK45963.1"
                     /translation="MRRSPLKVLLLASLLPLMTGCPGSTLKRELAGIKQTIDTARKNG
                     AYLCAPVELAMAESHHDMTAADLYYGDYFSAKEHIAITKDNADKAMAKTPPDRCAYKE
                     EKSVVVPKSGDADGDGIKDELDKCPDEPEDKDGFEDEDGCPELDNDKDGIYDSQDKCP
                     NDPEDKDGFEDTDGCPDPDNDKDGVLDKDDKCPMQPGPKENQGCPDTDKDKDTVVDRL
                     DKCPDDPGPPSNAGCPEKKYITITQEKIELKRQVQFATAKAIINKDSFELLDEVVELL
                     KKNPQIKLRIEGHTDTKGKPAKNLPLSQNRAKAVVDYLVKGGIDASRLSSEGYGQTCP
                     IADNATEEGREKNRRTDFFIAEPGAASKCATPDPKTGLVKAAAPAKAGKAGAAEAPAP
                     KGKGKKKKGK"
     gene            complement(10252..10581)
                     /locus_tag="PKL17_14345"
     CDS             complement(10252..10581)
                     /locus_tag="PKL17_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45964.1"
                     /translation="MAPYEYTAAVEYHRRAKELAGYARFHDANNFAKKARELAAAAKT
                     VAAKRNKNRALPIFDPSDPSLFITRDGLVKRKSSLEFDTEKPPMLDAEKPPLVGPMGN
                     EQKGGVK"
     gene            complement(10858..11727)
                     /locus_tag="PKL17_14350"
     CDS             complement(10858..11727)
                     /locus_tag="PKL17_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45965.1"
                     /translation="MNSSVSPYCALRFSLSAQKNWRALALLIVTLLLFPLGLFVGLLL
                     SRWAVRLDVAGSGESTSSSDRAAPLSAVSVTQGRRLSFLRWLLASPVRQSLWPVLSLS
                     IGIWLPAALCIYREAPDWSFLYLLPPDRATPVFLAVVFTVIGIAIPTGFVLGLFWLHR
                     IVLRGLQAVSSGTKERGDAQRVSKLWRLLFWLLVSLPLLGTLLAMRVGSERLFWVGSY
                     ADFHDGRWLLLPLIGGSQGSLRLLGIVALALLAMCAGCLVTLHALGSGRTGRSKRASS
                     DEISVSSSGFQAS"
     gene            11808..12062
                     /locus_tag="PKL17_14355"
     CDS             11808..12062
                     /locus_tag="PKL17_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45966.1"
                     /translation="MNALINYLIDNLILDFQGDLSMDEIRDFLRDDKSRDAERLRGKL
                     VEDRTTSQMMLTLADCLKEHLRTGINEDVIREQLRMYTES"
     gene            12275..12970
                     /locus_tag="PKL17_14360"
     CDS             12275..12970
                     /locus_tag="PKL17_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNK45967.1"
                     /translation="MTLLGVTLSSPLDMPQPVNPVITLAFSDIVDPDTGSLVALRLGG
                     KGSPLENLIEHNLVERKLILRPKASLVPNTEYQIDVSTSIKSLAGVAIDKNSRVTFRT
                     GTAVLPTTTEPPLVLADIIGDKGQLKRSCTATGCHSAQSPGEIAARGLDLSQATPTLR
                     SYLTSANASGSPEALRLVQPGQPERSYLLRKLLAGGSFTRIVGDPMPGEGSPLPETSA
                     LLAVQSWIRQGAN"
     gene            12982..>13748
                     /locus_tag="PKL17_14365"
     CDS             12982..>13748
                     /locus_tag="PKL17_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNK45968.1"
                     /translation="MRLWLVIAWLLVSDTAAAHKPNGARPAATDKWSDESCDDQEPST
                     LVTAIERELDVLRADPDATRVQHGRRKLSNLAYVEKLLLPIKHAAQKGKRALCRLLHK
                     KLELRKVATGNAHVTAYYHPVLRGSRTAQGPFQVPLYRRPSDEKLVTEPTAAILDGAL
                     RGQGLELLYVETLAQALHIHIEGSATIAFEDGSEVNLTTDGHNGHPYQNPLRLLLKDK
                     RIPDDFQPPPSQSRTQAYVTAHPEVLREYWGKNPHFV"
BASE COUNT         2791 a         4257 c         4066 g         2634 t
ORIGIN      
        1 agataaagtg tggatcgacg aggaacgtac ccgctgcggg cttgtcacca cgccccggat
       61 gcggctccgt cgtattggtg ttgtcggcgt tgtacggacg acggtacaga acgccatcga
      121 tgcacgccga aaagtaatgg cgcgtgttcg ccagaaaatc gtccgacatg accggagtgt
      181 tcggatcttt cttgtttcga caaagcagtt ggtaaaacaa gatgcccgat gtgctctcgg
      241 gaacagacca gtagaccatc aatccgccgt tgccactgcc caccgattca atcttgggag
      301 ccgtcggtcc tgcagtgttc accaccaggg gcagcttgca ggtcgccggg gaggaatcgg
      361 gtccaagcag cacctgggct gtcacgttct gcggaccgcc cgtgtcgcag acgtattcat
      421 atcccgttgg tgacgtaccc acaggccggg gcttggtcag cgcttcggcg ggcagcacaa
      481 tgtccaccgg ggaacccgcc actcggaagc tctcgggttt aatggaaaag acggtgttct
      541 ctggtggatt ggtcgatgat atctgctcgc actgcgcccc cgtcgtcgtg cgctgcgtct
      601 ggtccgagca gttgtcctgt ccgacgtaca tcgagctgtt gaacggctga ccatccgacg
      661 caaccggcga gtcgaggatc acgcgcatct tcagcccgcg tccgtgacat tgacactcgg
      721 ccttcccaaa cgccgtgccg atgttctgct gaacgatgtc gatggaatcg ctgctggccc
      781 cacaacccac cgactgcgga cccgccggcc cggtgttcag ttcgacggga acaccaccga
      841 gcgacagcat cgtccgtccc gagatggtct tgccattgac cgtcccagag cacgagccat
      901 ttcccgcagt cgagctggtc gtcgtggtcg tcgaccccgt cgtctgtgcc agcgcccaac
      961 tgggaagcaa cataccgagc gaaagtacca agatttgtct tgtcacttgc gtcatgtatt
     1021 acctccgaag caagagccgc gccaactttg cagcccgctc tccgacggaa tgggccagcc
     1081 ctcgtcggtt tgctgggaca ggctgacagc gcgcctgttc gatggcgcga caaagtgtca
     1141 cgccacgtcg ccgtccgcgg cgcaacacca ctccagtaca aaacgtatcc agcgacggag
     1201 caagcgtctg cggagacggc agcgactcgc caattcccaa gacattccca ctgccccagg
     1261 gcggacgcgc ttcggctgca cagacgaccg agctgacggt gcgctggtaa agctgaagtc
     1321 ccatcgcagg tgtctgttcg tgctgctgcc attccgccag caagcggaaa ccttcctgct
     1381 gcatcgactg cgccagtgca cttggctgat agcgcttgct tcgccagacg gaaaactgcc
     1441 gcggtcgact gctttggccc tgtacttcgg cgcgcaactc gaccgcatag ctcgacggaa
     1501 tggcgcagct tgcaagatcg atggctgtcg acagccgcaa gtcgcacggc gcctgcacat
     1561 ggttgcgaaa cagcgtctcg gccaggcgaa ccagctgcgg ctccgcgcgc ctggcatccg
     1621 ccaacatcgg atcccgttgc tgtaggtatc gccgatcctg cgctggaccg gcagcgacat
     1681 caaaaccaag caacagcaag tcgaggggac gcgcaagcca cagcgtttgg cgaagctgca
     1741 gcatttcccc gtcgagcagt ccaaattgag gagcaagcag cgtaaacagc cttggatgat
     1801 cgcctgacca catggcgtgc tgcggcagct ccgtcagcgc cgcttgcagc ccggccagtt
     1861 ggatcgaccg aacatcgcca agctgcatca cggcgcgacg aagcgcggca ccgagcagcg
     1921 cggaactctg ttccatcaaa aggacgcgca tccgcaatag accatctgac agcagccaac
     1981 cgagcagcga gatttcgtcg cgggcatccg cgcaggccag cgccagaaca tccagcgtcc
     2041 gccgaccgac cagctgccgc acgacctgtg ccacgtcttt ccaaagccca cgctgcacaa
     2101 gcggatcgtg acgattgcga aaatcggaaa acaccgtcgc acaagcggga tcgctcagca
     2161 gccatggcag cggcaagtaa ccgccttccc cggcaagtgc ccgccgcaaa cgttcataca
     2221 gatggagcat gtcgtctgag cccagccaaa cgcccggccc ccgagccggc gcaaacccga
     2281 gctgtggcgt aacagccaaa gacggtccaa tcggcggatt ggcaggcccg agcggattca
     2341 gcaccgtcgc aatcgcctgc aaggtctgcg cgcagggcac atgccgtcct gtctccaaat
     2401 tccgaatcgt actctcggat agaccacatt tatgtgccaa ttctgcacgc gacaaaccag
     2461 cacgctcccg ctgcctgcgc aaagacgcca tcaaccaatc gccagacacc catttttctg
     2521 gaagagaaaa cggatctggc accgctgacg gatcgacctg attcacgtct ttccgtgtca
     2581 cctgggctgc ggttttcgtg tgccagcgat gagaatccga cagtgatgcc ccggttgcca
     2641 agcaaatttc ctccacctgc gatttctgca atttcgatat gatttagtgg aacttaaacg
     2701 actgcgcaag ccatttttga cgatttcaca atgcacaact agcatattat gtgtaaattt
     2761 ttcccatcaa catatttatc tgtgcagata tttcccacta agcacatttt cgttcttatc
     2821 tggcaaaaag ccttgaccta catgacaagc tgcgtacact ccgctgcgat ggtgacgaaa
     2881 aagacagcag ggcgtggttt ctttttggtc tttgaaggca tcgacggcgc cggcacgacc
     2941 agccagcagc gctggttgtg tgaggagctg gaacgccagg gtatgcaggt ccacaaaacc
     3001 gccgagccca ccgcaaatcc cgtgggtcga ctcatccgtg aggtacttcg cggcgagcac
     3061 gctccctttt cggcgcatgc gctggcgctg ctgtttgccg ccgatcgcgc cgatcacctc
     3121 tcgcgggaga tcgatccggc agtattggca ggtaaagtcg tcgtcagtga tcgctatgtc
     3181 ttgtcgtcgc tggcttatca gacaatggcc ggtgtacccg acgatttggt cgcacaagcc
     3241 aatgcaccgt ttcggacccc ggacctgacc gtatatttcg atcttcccgt cgaggttgcc
     3301 gcgcagcgac gagccaagcg tggccaagcg caagaaattt tcgaggtgga tgacttccag
     3361 cggggagtcg cgacgcgcta cctagaccac gccaaacgct ggcagcgcga aggcaagccg
     3421 attgtttttg tcgatgcggc gcagtcgttt gatgcggtga gtcggcagct ctacgacctg
     3481 gtgctgccac atctggttgg cacgggagat cagagatcgg gaaccatcga ctgatcgagc
     3541 ggcggcatcg ccaggtcagg cagcggcatc gccaagtcag gcagcggcat cgccatgtcc
     3601 ggaaccatcg acagatcacg cggcttggcc atgtcgagcg gaggggtcac gggaagcggc
     3661 gggaacagcg tgagcttgcg ttgcgcgtgc tgaccattgc gcagatcgag tgcgatctcg
     3721 gcactcacac cgttgacctg gccaccgagc agactgcgca cactcacact cgcgggaccg
     3781 tcgggccata gcagcgcaac tttgaacgga aagtcttggc gagcgccaag cggcgtcgtc
     3841 ggagaaagcg gctgatcctt gcgccagatc agtaccttta gttggtcagc ggaagccact
     3901 ccgtctgcgc catcgagcgt caacgacagg cacgtctcgg tccgaccttc gcaagcgtcg
     3961 ctggaatccg aacaccccgc gcccaggccc acgctcagca gcgccaccgc cagcagatca
     4021 gcagttcgag ttttcatgat caatacccga atgtctgcgc aacaaccgac ggcatccgca
     4081 cgtcaatcgg tccgccaggc caagtcgtca ccgccgggaa gctctcggta gggggcgtcg
     4141 agcgatcgcg caggccaacc ccgagcccca caccaagtcc gatccccact gcggcgacgg
     4201 cgcccacgcc ggtccaaaac caccacttct tgggcagatc gcgaaagttg aactcactgc
     4261 cttttgtcgc cggttccgct tgggtcgaca cgctctgctg gttcgccgcg gtggtccgca
     4321 gcgccgggtc ggcgtcttgg tttgacacct gcggctcgag gaatttcgag cgcgcactcg
     4381 gttccagctt gatggctttc tgctcgcccg gcttgatgtc gatcaccacg gttgcgccgc
     4441 tggatgggtc cgtcggtgcc ggcaccatct gaagttcatg tttgccgggg tttaggcgca
     4501 gtggcgtttc gatcggcgac atcccgatca ctctgccgtc gagcttgatc tgcgcgttct
     4561 gccgcgatgc ctcgatgttc acggtcccag cgacttgatc gagcatcacc ccaagcttgg
     4621 caccaatttc gcgccgtttt tggtcgggca gcgtcggtga ctctttcagg aagcgaccaa
     4681 gcgcatcgag cgcatcgagc ggacgcccga cctctgcaaa cgcgagcccg agcggcagaa
     4741 gaaagcgcgg atttttgctt agcgaatacg ccttgccaag ctccgtcacc gcaccggctg
     4801 catcatgcgc cgcgagcagc cgttctccct ctttgccggc ccgttcggac tcggctttgg
     4861 cgtcggtagg cgcgggggct ttggcctgcg caaatgccga cgaagcggta aaaagaaaag
     4921 caccacatgc aaagacggta aggcgtcgct tcatggcgat ttttttgcac ggagagaggc
     4981 agagcggcaa gagcaaatag gcgacaaagt gggatgtttg ccacccggac cagaccgccg
     5041 cgtttctaaa ggcagtctgc gacgaaagaa atcaagcgca cgaaatctcg gtgtaaccct
     5101 tgcccgattc cagcgaacac cgtaccgttc taagtggggt tcatgtcgac cgagaacgaa
     5161 tgggcggcct ctatgattgg ccgcacgctc gatggtcagt accgactcga cggtatcttg
     5221 ggagaaggcg cgatgggcgt ggtcttccga gcaaccaacc ttgccgtcga caaggctgtc
     5281 gccatcaaga tgatgcgcaa agagacgttt gcggagccag acgcggtcga acgcttcaag
     5341 cgcgaggcca agctctggag ccagctcaac catccgtcga tcgcgcaggt cttcgacttt
     5401 ggtctttctg acgacatgcc ctacctggtg atggagctca tcgaaggcga ccagctctcg
     5461 gatgtgctcg atcgcgaagg tccgctgccg ccgctgcggg cgatttcggt gatgcgacag
     5521 ctcgccgcag cgctggaaga agcccaccga cacggtgtcg tacaccgaga cataaagccg
     5581 cagaatttga tgctgctccg ttacaagacg ggggggcggc tcacgctcaa ggtgctcgac
     5641 tttggcatgg ccaagcaggt cggcagcaaa gcgatgaccc tgacagcacc gggcattctg
     5701 gtcggaacgc cgaaatacgt cgcgcccgag caagtgaccg aaaatccgaa aatcgacggg
     5761 cgaaccgacc tgtatgcggc gggcgtcctg ttttacgaaa ttttgaccgg gcagccaccg
     5821 tttgtgggaa cgccacatga tgtcctgttt gctcacctgg gtcggcagcc tgagccactt
     5881 cccgacagca ttccgcagtg cgtgacggat gtggtgatga agcttctgcg caaaaaaccg
     5941 gcggaacgct atcaaaatgc cgtcgagcta gagcatgcgc tggaaacctg tgaagatgcg
     6001 ctgcgcggcc cgagcatctc gtcggcatca aatccggtgc tgaccgaaag cacggtccgg
     6061 cgagcgattg agacgcgacg aggacatcgc agcggcaagc gcaagctcgg cgaggcagga
     6121 ccgcgctacg gactgtgggg actgctcggc gcgcttggca tggcggcctt tggactgctg
     6181 gcgctgtcgt cgagcttgtc gcttcagcga aagcttgcgt cggtgatgtc agtgtacaag
     6241 gtgccgcaag acgagacggt gcaaaacgag ctcgtgcggc tccagtccga agcccagctg
     6301 cgcgcgtggc cggtggtgtt gcaaggcacc gaggacttgc agagaaccta tctcagctcg
     6361 ctgcttccgt cgcagagcag ccaagttcag gcgctgcgtc aaaaagcgct gctcgaacag
     6421 ccgctccagc cgatctatga acggttccaa gctgccgtcg agcgcaagga cgtcgaagaa
     6481 gccatccgac tctacgccca gtttagcaat gactcggtgt atcgcacgct ggccgagccg
     6541 gtgttcaatc agctgagcga ggcatttgtc tcgcagcatc ttcagcgcgc gagcagcctg
     6601 cgaagcgagc atcgctgccc ggaatttcaa gccgaagtgc agaagattct cgacgccatg
     6661 cccggacatc cgctggctcg ggccgagcag cgcaaagagt gcgtgcccca ggccatcgaa
     6721 cccccgcagc agccgggaac cgcgcccgtt tcgacgccca ctccggccct gcccgcccca
     6781 agcaagcagt aatccgcgct gtccgtcgca gagcaaaccc cgtcttttta cgtggctcgc
     6841 cccgccttgc ccgcaggcaa agcagaggcg gaccgttgag ctgactggga tttagaacag
     6901 gaactgcgga ccggcgtagg cgtcgatgag caggcctgta ttgccgctca gcgacgtgag
     6961 caggttgaca tcaagcgaga caccggcgtg acggccaccg acgtagaagc cagcgccagc
     7021 accgacgaac agcttgccaa gcaccaccga gtcaatgcac gaaccacccg tcggacaaac
     7081 ccgattcagg tactgggccg aaggggtgcc ggtttgctgg ttgtagccga tgttgccgcc
     7141 gacggacgta cagcccgcgg gcttgcgctc ggcatcggtg gttttgccgt tgtattccgc
     7201 actgcactga tcggtcggtt gcagtggcga atcggtgtcg atggcgccgt tcttcatcgg
     7261 tcgcgagccg tcgatgttca gaccgccgcg ccattcaccc ccaccgatgt cggcgtggat
     7321 gtacggacgg aagatccgcc cagggctaaa ctgataacga agcttgccga gcaccaagta
     7381 gccgaactgt cccttggctg tcgtcgcaaa gcacggcgaa ggacgacctt ggctgtcggt
     7441 gcagaggttg ttcttcatcg ccgagtcgct gctgtcggca ttgtgattga gcatcgcctc
     7501 gaagcgaccc gacacgccga gagagagacc gcgccacaaa aacgcaccaa actcggcgcg
     7561 aacgcccagc ccgctccagg tgaagcccgt cgacgatgac ttggcctgct cgtatttggt
     7621 ggtcttcggg ttgtaaaacc acgcaacttc agtggtcgtg ccggacgtga tgagcgcgcc
     7681 gccggtgcca attccaaggt cgaaatagac gcgatctttg agcccggcca aacgctgacg
     7741 ctcggcttct tgcttgacgt acttcatcgg gtcttcgacg tcttcgctct gggcgacgac
     7801 gatgccggca tacggcgaga ccgtcatgat gattgggttt tcggcaacgc cttgggttcc
     7861 gcagggacgg cctttggcgt cgtaaccctc gatgtagtac tggatcgact tgccgagcag
     7921 cgcatcgggc gggatcacac cgacgagatc gccgggctgc ttgcggctcg gaatcatcgg
     7981 cgattcggtg aagcgctctt ggccgggtga acgatagaaa atgctcgccc gcgcgatcgg
     8041 aatcgacgtc ggcgtgatgt agagattgac cttggtcttg gtccgcgttt ccgaaagcgg
     8101 cgtgtgacga agctcgcact tcttcaccgg gtcgagcagg tcgtcggcgc tgtactccag
     8161 cggctgcttc ggcggttccg ggcgcttcgt ttccacgtcg agcagcggat cactctgcgt
     8221 cggttgggct ggctgcaccg gctgcgcaat cggctgctgc accggctgcg caatcggctg
     8281 ctgcaccggc tgtacgggct gcggcatggg cgcttgcgcc gtccccgtcg cgattgccca
     8341 gactgtcttg acttccggcg tcgccaagtt cggatccagc ttcgcgttcg gattgatgct
     8401 gagcgcgcgc cggaaccatt gctcaccgcg gaccgagtcc ttgagctgaa cgtagaccat
     8461 cccgaggttt atatatgtcc gcgccgcttg gatcgtccct tccagggcgt gcttgcgcaa
     8521 aagcgcgacg gtgtcttcga gcgtcttttt cgccgcattg aactcgaggc gatcgtagtc
     8581 atccatcgcc tgcttgttca tgttggcgat gcggtcctgc ggccccgcgt gcgcgaactg
     8641 acccgacccc gccacgagca ccccggcgag cagccagcgt gccgagcgac ccatttttgc
     8701 cgacgacgtt tgcttcatcg attgcgccat gaaaaccacg tgcctcgaga cgtagattca
     8761 cccggcatct atccaaccgg gctcaacttg agattacagc gttttgggtc agtggtgcta
     8821 gcggagtcca acgcttttcg caacgctaca ccctgaaaag acgctgtttt ctgtcgattt
     8881 gatgacttgg tcgaccggaa tcggccctgc gcgaatgccc cagcacgtac cgcgaacgct
     8941 tctgcgaaaa gcaaaagcgc cgcggacttc gatgctgtaa ggggaaaaga catcctgctg
     9001 gaaggagatg ttagcaaggc cagtgccgtc gagaagtcag ccgttacttg ccctttttct
     9061 tcttgccttt gcctttcggc gctggcgctt cggctgcacc ggctttgccg gccttggccg
     9121 gggccgccgc tttgacaagt ccggtcttcg gatcaggcgt cgcacacttg ctggcagcgc
     9181 cgggctccgc gataaagaag tcggtacgac ggttcttttc gcgaccttcc tcggtggcgt
     9241 tgtcggctat cgggcaggtc tgaccgtacc cttccgacga caagcggctt gcgtcgatac
     9301 caccttttac caggtaatca acgactgcct tggctcggtt ctgcgacagc ggcaggttct
     9361 tcgcaggctt gcccttggta tcggtgtggc cctcgatgcg cagcttgatc tgcgggtttt
     9421 tctttagcag ttccacgacc tcatcaagca gctcgaacga gtccttgttg atgatcgcct
     9481 tggccgtcgc aaactggacc tggcgcttga gctcgatctt ctcttgcgtg atcgtgatgt
     9541 acttcttttc cgggcagccg gcgttgctcg gcgggcctgg atcatcagga catttgtcga
     9601 gtcgatcgac cacggtgtct ttgtctttgt ccgtatccgg gcagccctgg ttttccttcg
     9661 gacccggctg catcgggcac ttgtcgtctt tgtcgagaac accgtctttg tcgttgtccg
     9721 gatcggggca gccgtcggtg tcctcgaatc cgtctttgtc ttccggatcg ttggggcact
     9781 tgtcctggct gtcgtaaatg ccgtccttgt cgttgtcgag ctcggggcag ccgtcctcat
     9841 cctcgaagcc gtccttgtct tccggctcgt cgggacactt gtccagctcg tccttgatgc
     9901 cgtcgccgtc tgcgtcgccg ctcttcggca cgaccaccga cttttcttct ttgtaagcac
     9961 agcgatccgg tggcgtcttc gccatcgcct tgtcggcgtt gtctttggtg atcgcgatgt
    10021 gctctttggc cgaaaaataa tcgccgtaat acaggtcggc agccgtcatg tcgtggtgcg
    10081 actcggccat cgccagctcg acgggggcgc acaggtacgc gccatttttc cgcgccgtat
    10141 caatcgtctg tttgattccg gccagttcac gcttgagcgt cgaaccaggg cagccggtca
    10201 tcagcggcag aagcgacgca agcagcagca ctttcaaagg cgagcggcgc attacttcac
    10261 ccctcccttc tgctcattcc ccatcggccc gacaagcggt ggcttctctg cgtcaagcat
    10321 cggtggcttc tcggtgtcaa actcgaggct ggacttgcgc ttcacaagtc catcgcgggt
    10381 gataaacaag ctgggatcgc tgggatcgaa gatcggaagc gcgcggtttt tgttccgctt
    10441 ggccgcgacc gtcttcgctg ccgccgctag ctcgcgtgcc tttttggcaa agttgttggc
    10501 atcgtgaaac cgagcgtagc cagccaactc tttcgctcga cggtgatatt ccaccgcggc
    10561 ggtgtattcg tatggtgcca tcgaatcgga ccggctggct tttacctcag caatctcacc
    10621 ggtagcacca aacgttaccc gagatacgta agtaactgga ccacaagccg agcagagagc
    10681 aagccagggc agagcaccca gcgcgagcag acgcgatact cctgtcatag gcgacacggt
    10741 aacagggggc gaaggcaccc gtcaatactt acgatttttc cgtgggatgt agcattaccg
    10801 aactttttgc ccgtgcggtt tctgtccggt tgtccgcgca gtcctcgtcg cagcgggtca
    10861 agatgcttga aaaccagatg aagacacgga tatctcgtcg gaagatgccc gttttgaccg
    10921 cccggtgcga ccggacccaa gtgcatgcag ggtcaccaga cagcccgcgc acatcgcaag
    10981 gagcgcaagc gccacgatcc ccagcagccg cagcgagcct tgcgaccccc caatgagcgg
    11041 caaaagcagc cagcggccat cgtgaaaatc ggcgtaggaa ccaacccaaa acaggcgctc
    11101 ggacccgacc cgcatcgcca gcagcgtgcc cagaagtggc aaggatacaa gcagccaaaa
    11161 cagaagccgc cacagcttgc tcacgcgctg cgcatccccg cgttcctttg tacccgatga
    11221 cacggcctgc aagccccgca ggacgatccg atgcagccaa aacagtccca gaacaaatcc
    11281 cgtcggaatt gcaatcccaa ttacggtaaa aacaacagcc aaaaacaccg gggtcgcacg
    11341 atcgggaggc aaaaggtaca gaaacgacca gtcgggcgct tctcgataga tacacagcgc
    11401 agccggcagc cagatcccga tagacagcga cagaaccggc cacagtgact gacggaccgg
    11461 cgaagccaaa agccagcgca gaaacgaaag cctgcgaccc tgcgtgacgg aaacggcaga
    11521 cagcggcgca gcacgatccg acgacgaagt ggattcccct gaaccggcca cgtcaagccg
    11581 cacggcccac cgcgaaagca gcagcccaac aaacagtccg agagggaaaa gcagcagtgt
    11641 cacgatcagc agcgccagcg cacgccagtt tttttgtgcg gacagtgaaa agcgcagcgc
    11701 acagtacggc gaaactgagc tgttcacaag tggctaaact cgtgatatca ataccaaatc
    11761 ggtactgtct tttctggttc aaccataccg cccctatgga ggcagatgtg aacgccctca
    11821 tcaactactt gatcgataac ctgatcctcg actttcaggg cgatctgtcc atggacgaaa
    11881 tccgcgactt cctgcgcgac gacaaaagcc gcgatgcgga gcggctgcgc ggcaagctcg
    11941 tcgaagaccg gacgacatct cagatgatgc tcacgctcgc cgactgcctg aaagaacatc
    12001 tgcgcaccgg gatcaacgag gacgtgatcc gcgaacagct ccgcatgtac actgagagct
    12061 gatgggctct ctcctccgcc acccgccaca gcctcgactc cacactccgc acgctacaca
    12121 gacccagccg catgaccgcc ccgctgggtt ttgtgcaccc agccaaggcc gcaccaccct
    12181 cgcctgctcc gctgccgttt tttgggcggt ttcgctgggc gcttgcagct tcccgactga
    12241 cgcagaacca atcccgacgg ccagcgaagt gccgatgacg ctgcttgggg tgacgctgag
    12301 cagtcccctc gacatgccgc agccggtcaa tccggtcatc acgctcgcgt tttcggacat
    12361 cgtggatcca gacaccggtt cgctggtggc gctgcggctg ggcggcaaag gcagtccgct
    12421 tgaaaacctc atcgagcaca acctcgtcga gcgcaagctg attttgcggc ccaaagcgag
    12481 ccttgtccca aacaccgaat accagatcga tgtgagcacc agcatcaagt cgctcgcagg
    12541 ggttgccatc gacaaaaaca gccgggtgac gtttcggact gggacagcgg tgttgccaac
    12601 gacgacggag ccgccgcttg tgctcgcgga catcatcggc gacaaaggtc agctgaagcg
    12661 gtcatgtaca gcgacgggct gtcattctgc ccaaagtccg ggcgaaatcg cggcgcgtgg
    12721 tcttgatctg tcgcaggcca cgccgacgct gcgcagctac ctgacgagcg caaatgcgag
    12781 cggttcgccc gaagcgctgc gccttgtgca gccgggtcag ccggaacgaa gctacctttt
    12841 gcgcaagctg ctcgctgggg gttcgtttac gcgcatcgtc ggcgatccca tgccaggtga
    12901 aggaagcccg ctgccggaaa cgtcggcact gctcgcggtg caaagctgga ttcggcaagg
    12961 ggccaactag gaaaccgacc gatgcgactt tggctggtca tcgcatggct gcttgtgtcg
    13021 gacactgcgg cggcgcacaa gccaaatggc gcacggcccg cagcgacgga caaatggtct
    13081 gacgaaagct gcgacgatca agaaccgagc acgctggtga cggccatcga acgcgagctg
    13141 gacgtgttgc gcgccgatcc agatgcgacg cgggtgcagc atggtcggcg caagctgagc
    13201 aacctggcgt atgtcgaaaa gctgctgttg ccgataaagc acgccgcgca gaaaggcaaa
    13261 cgcgcgctgt gtcggctgct gcacaaaaag cttgagcttc gcaaagtggc gacgggaaac
    13321 gcgcatgtaa cggcgtatta ccatccggtg ttgcggggat cacggacggc gcaaggcccc
    13381 tttcaggttc cactgtaccg tcgaccgagc gatgaaaagc tggtgaccga gccgacggcg
    13441 gcgattttgg acggtgcgct gagaggccaa ggactggagc ttctgtacgt cgagacgctg
    13501 gcgcaagcgc tgcacattca catcgaagga tcggcgacga tagcgtttga agacggcagc
    13561 gaagtaaacc tgaccaccga cgggcacaac gggcatccgt accaaaatcc gctgcggctt
    13621 ttgctaaaag acaagcggat tcccgacgat tttcagccgc caccatcgca aagccggacc
    13681 caagcgtatg taaccgccca tcccgaggtg ctgcgcgagt actggggaaa aaatccgcac
    13741 tttgtttt
//
LOCUS       DAORGN010000134        13638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1866_length_13638_cov_5.274534, whole genome shotgun sequence.
ACCESSION   DAORGN010000134 DAORGN010000000
VERSION     DAORGN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_13638_cov_5.274534"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PKL17_14370"
     CDS             complement(<1..1073)
                     /locus_tag="PKL17_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45969.1"
                     /translation="MPKMRWLWSLLFPLVSCSHDPSVDHVVPPTMPPVPSPPAIHFVV
                     RDGDSMMPMPARAIVYPTMGTAKPDFKSDGLTASVLAPGVIGSPEGVLLVSGDGTVGV
                     PAGTYDLWLLQGLEYELVKKSVVVAQDAVTEVDVTLEHSVKTGGYLSADMHIHTKRSF
                     DSKLLAAHRVISEVTSGVQVIVPTEHGFHNDLSELIKSLDYSLRAVSIPGSEYNFQGG
                     HAGIYPVVFDPNGQFWGAPPWQEWPKPNMADPETYFPLIHMQQGNPLVVVNHPRLPPD
                     LGYFLNIRWKPGMPLSTAGLFDGIEVLNGYAMRPPDIADLLLDWFALLNQGLRLTGLG
                     NSDTHRIDWLRGGYPRTWLGLPTT"
     gene            complement(1096..2214)
                     /locus_tag="PKL17_14375"
     CDS             complement(1096..2214)
                     /locus_tag="PKL17_14375"
                     /inference="COORDINATES: protein motif:HMM:NF015254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HNK45970.1"
                     /translation="MRSLLRGWLGPVALSFLLASCGGLPDQPVPDDEQSGSPSLPTVP
                     PLGAPITAPAKTWTWVNFPDAVCDDGSATGIGVSLNPASKDLLIFLQGGGACWDYDSC
                     AVSNTSTHGPYGESQFNSTKLLFVIGSILDRNAKSSFQDMNMVYVPYCTGDVHTGDNV
                     MTYDGGGDKKRVIHHKGRPNLIAFLKRIAATVPMPGKLVVTGSSAGGFGSSLNYDLVR
                     AFFPQAKSYLLDDSGPPMVGDAIPADLRQKWYTAWRLDQTIGKLCPDCNRDFSTLVPI
                     LGKRYPHDRMGLLSYTQDGVIRKFFGNQSEQQFQQNLTAMAKSTIDPLPNFHYYFVSG
                     TGHTFLFTPGLTTVKGVSLLGWISQLGSDDPKWTSVTP"
     gene            complement(2561..3565)
                     /locus_tag="PKL17_14380"
     CDS             complement(2561..3565)
                     /locus_tag="PKL17_14380"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45971.1"
                     /translation="MKTHVNFICWGATIFGAALGLSEGSAQAQQRVAFGSSAIHGGTD
                     SRLTRHEPVVTVEPLDHLPRLRPVLRGAMYRSGTPSEAALAHLCERGWKRVYSLYGEH
                     TTQTGPRNVNMLRTGRDLRSCQMDGSQRTIEWRSAPSARLRTLPNIFRDVLESIRNPD
                     KGPVLVHCWNGLHYAGMVSALALRQFCGFSAEQAEAYWRANANRGANYPLIIANLHNF
                     KPIPGLSLSPQEQQAMCPDVSTGYMLPTDNVVAAALIHRGKGGSHNPPIPPLRNAMES
                     SEGSASSAPLPHDHGRSGASDRSQVPASAGPAATSGATSDNTQIPAPHLGPKLAARRS
                     "
     gene            complement(3764..6001)
                     /locus_tag="PKL17_14385"
     CDS             complement(3764..6001)
                     /locus_tag="PKL17_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45972.1"
                     /translation="MSASRPLPRKGPSTTRPERTRCLLRKPTNILFDSAGHARLTDFA
                     MVWPADAAQKQESRTDRTVYMAPEEQAGGNLGDPRSDVYSLGMCALYSLLGKELTPAM
                     VMDRATLIEQLDAPPPLKAVLLRAVAPNPNDRFPTVAEFCRALEFDAPALPGMVTRAS
                     LPGVPAGLTRTNPGLMAPGVRQSSPQMPVMAPLSRTNPPINPSLTRTNPGLFGGQAPP
                     LTSPAKPAGVRTTGPQPLAGARPVTPPPPAANLRTTNPPPAANLLKTNPPMPGLTRTQ
                     PPPPVRVTTGEEEIQPPPGRSAGEVAEYAIDTERVRAVTAEAMTSRNPNSVMQPLPTP
                     PPRKQWPLMVGFGALVLVGGVGLGTYLVNSTRKPVVAVTEPVVGKETPAGPNGAAAVH
                     PVEPGSEDTFVATPIIPVKPGEVASSKNKHDADSKAHSAQAAAKVAAKTDAESAKHAD
                     AKATQAAPAKAVAGAEKDIKDSGEKVAQRPSLMKSVAQKAEPVKPPEHKPLLAKTDAV
                     KADAGKPVAQIKPEPAKVVAAVKPEPAKPVAQAKLEPAKVAQAKPEPAKPVAAVKVDA
                     QKLVAQAKLDAQKPMVAAKPDPLKLAAAKPEPAKAVAPAKPVVAVKPVAVAKVEPAKP
                     VAKPIAAYKPMPVKPMVAQAKPAPSAQATPEGQAALRDAQLAFVRGDRQQALAMALGV
                     TRRGGEDAASAWRFVGGAACSNRQAALATTAYRNLKSPDARKTLIDLCQRNGVPFAGG
                     QFSSD"
     gene            complement(6045..6887)
                     /locus_tag="PKL17_14390"
     CDS             complement(6045..6887)
                     /locus_tag="PKL17_14390"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNK45973.1"
                     /translation="MTSADIKTSKPACSAARLPVVYLPHGGGPWPFVEMGLPRQELDS
                     LASYLRAVRALPLAAPKALLVISAHWEEPVPTVMTAARPPLLFDYYGFPPASYKLTWP
                     AAGDPLLAARVLELLRAAGIDGKTDEKRGFDHGTFVPLKLTYPDADVKVVQLSLQAGL
                     DPALHLAMGRALAPLRDEGIFIIGSGMSFHNMRAFGSPRAHDMSFAFDAWLRESMLLP
                     PAEREARLRSWASAPQARLAHPREEHLLPLMVCAGAAGDSQGTLGYSGSMMGARLSAF
                     HFVA"
     gene            7142..8914
                     /locus_tag="PKL17_14395"
     CDS             7142..8914
                     /locus_tag="PKL17_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45974.1"
                     /translation="MDRHPPRTTSEEIDLYIRTYYSLLRSSGEVRVRAFEEAHIYSNS
                     SLHEGSRDPAPDVAAFAYSSARLPACMFFVRRIVLGQSHEHFEAAGYQVREWQQVTTR
                     GRRRPLRFNGSDTLAAFVTSISDIDDLVPILTAFQIEWNKLHHLLAHSALSRKSAAAR
                     ELGSADPAAVTESLPAQEFIDDSDLEKTLSLDAQGMKTLKAALGESLGAGLREMATRE
                     QDLRVRLLVGSYSQYQRAAQRWWSGIEPQYLRQSRPRRPAVYFVSSNSHSVVNLLGGY
                     AKLHRQQLIQFAKQRNPEHLADSLSDALRSGNEAKVDNLLYYLLRSFLHDPLGAHHRE
                     RVREFDSESGIESLESPGRIDVSAQLVQLSSLRKERIDPRLLAGGVGDSFSLLQKSEA
                     VILNIDYPLGMAAYHLLSRLAQGVGEVRGIYVMGKAATLNGRVGDVMISSVVHDEHSL
                     NTYLLRNCFVSGDVQSYLEHGTVLDNQKALTVRSAFLQNRAYMGSFYHEGYTVLEMEA
                     GPYLSAIYEIVNPSRHPKNEIVHLSNSTPFEVGLLHYASDTPYSRRQSLLSKSLSYFG
                     VESTYGCTIAILQRILRSEMARLG"
     gene            8981..9916
                     /locus_tag="PKL17_14400"
     CDS             8981..9916
                     /locus_tag="PKL17_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK45975.1"
                     /translation="MPVHAEVQAFLQMLAAANLPPYEHSTVAQARERMNAIAMLERPA
                     SPLAEVRDLCIPAPHGDIPLRLYRSSLDQALPTLIYFHGGGWVVGSIESHDRLCRALA
                     SASGFAVISVEYRLAPEHKYPAAVEDAEAAYRYVRAFADKLGVDSESIFLGGDSAGGN
                     LSAVVSQRARDAGEPLPRGQVLIYPATGSAAVTESRKEFADGYLLTAGAMMWFTAHYI
                     SDLAQLRESSLSPCFAKDVSGLPPTFLLTAEFDPLRDEGEAYAAKLTKAGVNVTLRRY
                     DGMIHGFLQLSAMVSAATTAIEDIATWLKQTQRGT"
     gene            9913..>13638
                     /locus_tag="PKL17_14405"
     CDS             9913..>13638
                     /locus_tag="PKL17_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK45976.1"
                     /translation="MMTTWGDDVLATESPPFAGRFRLQRQLGSGGMADVFLSFDEETQ
                     TPCAIKLLSQHSDPEQRQRFFREASVLSQLQHPGIVRYHAHGYTEQGVPYLAMELLEG
                     EDLCQRLLRKPLTAAETLVLAQAVLSALTAAHQAGIIHRDIKPANLFLVSGEPARTKL
                     LDFGIVRLTTASQHTRTGSILGTPEYMSPEQLRGLKELDARSDLFSLGVVLYECLAGW
                     SPFRSDHPMGTLGRILFDDPPKLSERNPHLPEALCVLVHEMLAKEPRHRPPSAEAVRT
                     RLQNISVDSDGDRITGWRAPAALTHRELRWLSMIIFQPTRIDLALEKTSILEAGELDQ
                     DAIGQTAAAHSARREKLADGTQILLLDGNATAGEQAEQAALCAIELHSHDPHTAIAIC
                     FGQTDLGQHRLDAQALERAADMLDAWRKRTAAASTQKERSSAPILVDESLRRMLMSRF
                     EFVEQDSAWELTPTERRDELHVLGRVSPFAGRDTELTQLATALSACCEDSQAAAVLIT
                     AEPGMGKSRLCHEFLRQAKLSYPDLEVWIGAGDPSSAGSPFHLIAQAIRQAMGVQKSM
                     TQTEKTERLHSFVSGRCNTADHERIGEFLGELLGITYQQTPSVTLQSARSNPVLMGDQ
                     IRFAFLDLLRASMADHPLLLLLEDLHWGDLPTVQLVEAALRNHSESPLFLLALARPQV
                     DDLFPKLWDRARLLKLPLRPLSKRACEKIAKGILGNRFGADKLTKLAERAGGHPFFLE
                     ELLRATSESDSFELPESVLAMVQSRILRLDSMARRVLRAGSVFGRVFHRGGLTKMLGI
                     DHSVGSSESEQKSLRDALSTLVDREFVNPRHPSRFAGEEEFVFSHALVQESAYSMLTA
                     EDRQLGHRLAAEFLQAVGEDDAMVLAEHCTLGGVLDQAVMYLRQVVSEALTGNDFRAA
                     LSCTARALKLGAEGTVRGTLLAMEAEAHQWLGEFRAAIDSAEEALHLLPVGSVPWYRA
                     VDALSIGCGRLLLHDRLSELAVQLLALLHHQTESEHFSLPVLASSAVRLGMQLYICGR
                     YKDALALLQPIEALIDADPNPKPPALLASICVLRANRAAYEWKIDLCPDYFEQSANLL
                     DQIGDRRAACSQRLDTALFCSDIGDFVRMERLLREVIPVALTLNLLRVLGVSFSALGV
                     ARWRQGHHEEALTHCQKALAQLESLGDARQEGVCRINLARILYSLGQFEKAHAAALTA
                     SERLAEIPRFRVRALAWLARIEQQRGNLDAAALAAETS"
BASE COUNT         2661 a         4328 c         3983 g         2666 t
ORIGIN      
        1 gtcgtcggca aacccagcca agtacgcgga taaccgccac gcagccagtc gatccgatgg
       61 gtgtcgctgt tgccaagccc ggtcagccgc agtccctgat tgagcagcgc aaaccagtcc
      121 aacagcagat ccgcaatgtc gggcggacgc atcgcatagc cgttgaggac ttcgattccg
      181 tcaaacagtc ccgccgtcga aagcggcatt cctggcttcc agcggatgtt caaaaagtag
      241 cccagatccg gcggcaagcg cggatgattg accaccacca gaggattccc ctgctgcatg
      301 tgaatcaggg ggaagtatgt ctctggatcg gccatgttgg gcttgggcca ttcctgccaa
      361 ggcggagccc cccaaaactg tccgtttgga tcgaacacca ccggataaat gccggcgtgt
      421 ccgccttgga agttgtactc actgcccgga atggacaccg cgcggaggga atagtcgagc
      481 gacttgatca gctcacttag gtcgttgtga aagccgtgtt ctgtcggaac aatcacctgc
      541 acaccggatg tcacttccga gatcacccga tgcgcggcca gcagcttgga atcgaagctc
      601 cgcttggtgt gaatgtgcat gtctgctgac agatagccac cggtcttgac gctgtgttcc
      661 agcgtgacat cgacttcagt gacggcgtct tgcgcgacga ccacgctctt tttcaccagc
      721 tcgtattcga gtccttgcaa aagccacaga tcatacgtac cagcaggaac acccaccgtt
      781 ccgtctccgc tcaccagcag cacgccttcc ggtgaaccaa tcacgccggg tgcaagcaca
      841 ctcgcggtga gtccgtccga cttaaagtcc ggcttggcgg ttcccatcgt cggatagaca
      901 atcgcccgcg ccggcatcgg catcatgctg tcgccgtccc gcaccacaaa gtgaatcgca
      961 gggggagacg gaaccggcgg catcgtcgga ggaaccacat gatccacgga cggatcgtgc
     1021 gagcagctca caagcggaaa cagcagactc caaagccagc gcatcttcgg catcggacac
     1081 agaatagcag agcgcttacg gagtcaccga agtccatttc ggatcgtcgc tgccaagctg
     1141 agaaatccag ccaagcagag acacgccttt gaccgtcgta agtcccggcg taaacaggaa
     1201 ggtatgtccc gttccggaca caaagtagta gtggaagttg ggcagcggat cgatcgtcga
     1261 cttggccatc gccgtcaggt tctgctgaaa ctgctgctcg ctctggttgc caaagaactt
     1321 ccgaatcaca ccatcctggg tatacgaaag gagtcccatc cgatcgtgcg gatagcgctt
     1381 gcccaagatt ggcaccagcg tcgaaaagtc acggttacag tccgggcaca gcttgccgat
     1441 ggtttgatcg agtcgccacg ccgtgtacca cttctgccgc aggtcagcag gaatcgcatc
     1501 accaaccatc ggcggcccgc tgtcatccaa aagatacgac tttgcctgcg gaaagaacgc
     1561 ccgcaccaaa tcgtagttaa gcgacgatcc aaaaccgccc gcgctgctgc cagtcaccac
     1621 cagttttccg ggcatcggaa ccgtcgctgc gatccgcttt aggaaggcga tcagattggg
     1681 tcttcctttg tgatgaatga cgcgcttttt gtcgcccccg ccatcgtagg tcatcacatt
     1741 gtcgcccgta tgtacgtcgc ccgtgcagta cggaacgtag accatgttca tgtcttggaa
     1801 cgatgacttg gcattccgat ccaagatcga tccgatcaca aacagcagct tggtcgaatt
     1861 gaactgagac tctccgtatg ggccgtgcgt cgacgtattt gacaccgcgc agctgtcgta
     1921 gtcccaacag gccccgccac cttgcaaaaa gatcaaaagg tctttgctcg cgggattcag
     1981 acttacgcca attccggttg cagatccatc gtcacagact gcatcaggaa agttcaccca
     2041 cgtccaggtc ttggcgggcg cagtgatcgg agcccccagc ggcggcacgg tcggcaaaga
     2101 tggagatcca ctctgctcgt catcgggcac cggctggtcc ggcagtccgc cacagcttgc
     2161 caacagaaac gaaagagcga cagggccaag ccaaccacgc agcaaagaac gcatcacaga
     2221 cctccgtttt cgtcgcagaa tgtggccgca caggaatgca gaaagccaaa gcggggcacg
     2281 atgtctgcga ttagtcgcac agcttgcgac gcattgctac cgcgaaccga tgggcaaatc
     2341 gctgtagcag tctgtccaaa agcgaaatgg cggacggcga agcagagatc cgccgcaacg
     2401 actacgtcgt gatctcacga gaggcagagg gccgcatcgt tcgctatcga cgccagggcg
     2461 agcccttccc atcgctgacc atcgccgccg atcgccgcgg aagtctattc gggtgtgctt
     2521 ttggcttgcg accggatcgg caaaaaggga tgtggtctcg tcaggaacga cgcgcggcga
     2581 gtttggggcc caggtgtggt gccggaattt gcgtgttgtc gctggtcgct cccgacgtag
     2641 ccgccgggcc tgctgaagca ggaacctggg aacgatcgct tgcgccactg cggccatgat
     2701 catgaggaag cggtgcgctc gatgcacttc cttcgctcga ttccatcgca ttgcgaagcg
     2761 gcggaatcgg cgggttgtgg ctgccgcctt tgccccggtg gataagcgct gccgcgacca
     2821 cattgtccgt cggcagcatg taacccgtcg agacatcggg acacatcgct tgctgctctt
     2881 gcggcgacag cgataggccc ggaatcggct tgaagttgtg caggttcgca atgatgagcg
     2941 gatagttggc accgcggttg gcgttggctc gccagtacgc ctcggcttgt tccgccgaaa
     3001 aaccacagaa ctgccgcagc gccagcgccg acaccatgcc cgcgtagtgc aagccgttcc
     3061 agcagtggac cagcaccggc cccttgtccg gattgcggat gctttccagc acatcgcgaa
     3121 agatgttcgg gagcgttcgc agccgcgccg atggtgccga tcgccactcg atcgttcgct
     3181 gcgagccatc catctggcag cttcgcaggt cgcgcccggt gcgcagcatg ttcacgttgc
     3241 gcggtccggt ctgggtcgtg tgctcgccgt acagcgaata cactcgcttc cagccccgct
     3301 cgcacagatg ggcaagcgcc gcttcacttg gcgtccccga tcggtacatc gccccgcgca
     3361 gcacaggacg cagccgcggc agatgatcga gtggctccac cgtcacaact ggttcatgac
     3421 gggtcagccg actatcggtg ccgccatgaa ttgcgctgct gccgaaagcc acgcgctgct
     3481 gagcctgcgc gcttccttcc gaaagcccca gcgcagcgcc gaagatggtt gccccccagc
     3541 agatgaagtt gacgtgggtc ttcatgagct cccgacgaga atggatgaca atgcccagat
     3601 tatacagtgc tttttttgca tctgtaccca caaaatcacg gccttatgaa cgcagcgtgt
     3661 gagcccaact acccaagtcg tgggccgacc cgtaccgcac agtgaaaaga cccatggtgt
     3721 aggcgtttac gccccgccgc gatctgtagg ccatgcagta cacctagtcc gagctaaact
     3781 gcccacccgc gaacggcacg ccgttgcgct gacacagatc aatcagcgtt ttccgggcat
     3841 ccggggactt taggttgcgg tacgccgtcg tggccagcgc tgcttggcga ttgctgcacg
     3901 cagccccgcc gacgaaacgc caggcactgg ccgcgtcttc gccgcctcgc cgcgtgaccc
     3961 cgagcgccat cgccagcgct tgctgccgat cgccgcgcac aaatgccagc tgcgcatcgc
     4021 gtagcgccgc ttggccttct ggtgtcgcct gggccgaagg cgccggcttg gcctgagcca
     4081 ccatcggctt gaccggcatc ggcttgtacg ccgcgatggg tttcgccacc ggcttggccg
     4141 gctcgacttt ggcaaccgcc accggcttga ccgccaccac cggcttggcc ggcgccacgg
     4201 ctttggcagg ttccggcttg gccgccgcca gcttcagcgg atcgggtttc gccgccacca
     4261 tcggcttttg cgcatcaagc ttggcttgcg cgacgagctt ttgcgcatct accttcaccg
     4321 ccgccaccgg tttggccggc tccggcttcg cttgcgccac cttggccggc tccagtttcg
     4381 cttgcgccac cggtttggcc ggctccggct tgaccgcagc caccactttc gctggctccg
     4441 gcttgatctg cgccaccggc ttgcctgcgt ccgctttcac cgcatcggtc ttcgcgagca
     4501 gcggcttgtg ctccggcggc ttgaccggct cggctttctg cgccaccgac ttcatcagcg
     4561 acggcctctg cgccaccttc tcgccgctgt ctttgatgtc tttttccgcc cccgcgactg
     4621 ctttggccgg agccgcctgc gtggccttgg catcggcgtg tttggccgac tcggcatcgg
     4681 tcttggccgc cacttttgcc gccgcctgcg cgctgtgcgc tttgctgtcg gcgtcatgct
     4741 tgtttttgga cgatgccact tcacccggct tgaccggaat gatcggcgtc gcaacaaacg
     4801 tgtcctcgga acctggctcc acaggatgca ccgccgctgc accatttggg ccggctggcg
     4861 tttctttacc gacaacaggc tcggtcactg cgaccaccgg cttgcgcgtg ctgttcacca
     4921 gataggttcc aagtccgacg ccacccacca aaaccagcgc gccaaagccc accatcagcg
     4981 gccactgctt gcgcggcggc ggcgtcggga gcggctgcat caccgagttc ggattgcgcg
     5041 acgtcatcgc ttcagcggtc accgcccgca cccgctcggt gtcgatcgcg tattccgcga
     5101 cttcgccggc actgcggcct ggcggcggct gaatctcttc ttcccccgtc gtcacccgaa
     5161 ccggcggagg cggctgggtc cgcgtcagac ccggcatcgg cggattggtc ttgagcaggt
     5221 tcgctgccgg cggtgggttg gtcgttcgca ggttcgccgc tggtggcggt ggcgtcaccg
     5281 gacgagcccc agccaaaggc tgtggtcccg tggtccgcac ccccgccggc ttggccggtg
     5341 aggtcagcgg cggcgcttgc cccccaaaaa gccccggatt ggtccgtgtc agcgacggat
     5401 tgatcggtgg attggtccgc gacagcggcg ccatcactgg catctgcggc gaactttgcc
     5461 gaactcccgg tgccatcaga cctgggtttg tccgcgtaag tcccgcaggc acgcccggca
     5521 gagaagcgcg cgtcaccatc cccggcagcg ctggcgcgtc gaactccagg gcccgacaga
     5581 actctgccac cgtggggaag cgatcgttgg ggtttggcgc gaccgcccgc aaaagcaccg
     5641 ctttgagcgg cggcggcgca tccagctgct cgatgagcgt cgcccggtcc atcaccatcg
     5701 ctggcgtcag ctctttgcca agcaaggaat acagcgcgca catgcccagc gaatacacat
     5761 ccgagcgcgg atcacccagg ttgccgcccg cttgctcctc gggcgccatg tagaccgtgc
     5821 gatcggtgcg actttcctgt ttctgggccg catccgccgg ccacaccatc gcaaagtcag
     5881 tcagccgcgc atgtcccgcc gagtcaaaca gaatgttggt cggcttacga agcagacagc
     5941 gcgttcgctc gggacgtgtt gtgcttgggc cttttctggg aagtggtcgg gaagcggaca
     6001 acctgcccgc gtggcggacg tcgtgcgacc agagcgctga ccggttacgc cacaaaatga
     6061 aacgcggaca gccgcgcgcc catcatcgat cccgaataac ccagcgtgcc ttgggaatcc
     6121 ccggcggcgc ccgcgcaaac catcagcggc agcaggtgtt cttcccgcgg gtgggccagc
     6181 cgagcctggg gtgccgatgc ccagcttcgc agccgcgcct ctcgctctgc tggcggaagc
     6241 agcatcgatt cccgcagcca cgcatcgaac gcaaaagaca tgtcatgcgc gcgtggcgaa
     6301 ccgaacgccc gcatgttgtg aaacgacatg ccactgccaa tgatgaagat cccttcatcg
     6361 cgcagaggcg cgagcgcccg tcccatcgcc agatgcaggg ccggatcgag cccagcctgt
     6421 agcgaaagct gcaccacttt cacatcagcg tcgggatacg tcagcttgag cggcacaaac
     6481 gttccgtgat caaagccgcg cttttcgtcg gtttttccat cgattccggc ggcgcgcaaa
     6541 agctccagca cccgcgcggc cagcaaagga tcccccgctg ccggccacgt aagcttgtac
     6601 gacgcaggcg ggaagccgta atagtcaaac aaaagcgggg gccgcgccgc cgtcatcacc
     6661 gtcggaaccg gctcctccca gtgcgccgaa atcacgagca gcgctttcgg tgcagcaagc
     6721 ggcagcgccc gcacggctcg cagataggaa gccagcgaat ccagttcctg ccgaggcagc
     6781 cccatttcga caaagggcca tggcccgccc ccgtgtggca gatagacgac cggcagccgc
     6841 gccgccgagc acgccggctt gctggttttg atgtccgcgc tcgtcatggt gtacctccgc
     6901 gcctatgcag cgtgtgaacg tgggccgccc agtgcgccca tgttgccaag ataatttgtt
     6961 ccccaaactg cactagtgga caaaattgca acggactgtt gtttgtctgc taccaatggc
     7021 cgcggggccg cacgaccaat ttttgggccc ggtcgtgcgc ttcccattga ccgacaccac
     7081 agatcctctc atacacatgg ggcacccgcg tctttccaaa aagtgccaac gaggaattcc
     7141 agtggaccga catccccctc gcacgaccag cgaagagatc gatctgtaca tccgaaccta
     7201 ctactcgctt ctgcgcagtt ccggggaagt ccgggtgcgc gcgttcgaag aagcccacat
     7261 ctacagcaat tccagtctgc atgaaggcag ccgcgatcct gcgccggatg ttgccgcgtt
     7321 tgcttacagc tcggcgcgtc tgccagcctg catgttcttt gtccgccgca tcgtgctggg
     7381 acagtcccac gaacacttcg aggccgccgg ctatcaagtc cgagaatggc agcaagtgac
     7441 cacccgtggc cgtcgccgtc cgctgcgctt taacggcagc gacacgttgg cagcgtttgt
     7501 gaccagcatc agcgacattg atgatctggt tcccatcctg actgcgtttc aaattgagtg
     7561 gaacaagctg caccatctgc ttgcgcacag tgcactgtcc cgcaagtccg ccgccgcaag
     7621 ggaacttggc agtgccgatc cggctgcggt gacagaatcg ctgcccgccc aagaattcat
     7681 cgacgattcc gatctggaaa aaacgctgtc tttggatgca cagggcatga agaccctgaa
     7741 agcggcgctc ggtgaatcgc tcggtgcagg acttcgcgaa atggctacgc gggaacagga
     7801 tctgcgggtg cgactgctcg tcggttccta ttcccaatac cagcgcgccg cccaaagatg
     7861 gtggagcgga atcgaaccgc agtatctaag gcagagccgt ccccgccgcc ccgccgtcta
     7921 ctttgtttcg tcgaacagcc actcggttgt gaacctgctg ggaggctacg ccaaactgca
     7981 ccgtcagcag ctgattcagt ttgccaagca gcggaatccg gaacacctgg ccgactctct
     8041 gtccgatgca ctgcgcagcg gcaacgaagc aaaagtcgac aacttgctgt actacttgct
     8101 gcgttccttt ttgcatgatc cgctcggtgc tcatcatcgc gagcgggtgc gggaattcga
     8161 ttccgaaagc ggcatcgaat cgctcgaaag tccgggccgt atcgacgtga gcgcccagct
     8221 tgtgcagctc tcttcgctgc gcaaggaacg gatcgatccg cggctcttgg ccggtggcgt
     8281 gggcgattcc ttttcccttt tgcaaaaaag cgaagcggtg atcctcaaca tcgactatcc
     8341 gctcggcatg gcggcgtatc accttctgtc gcggctcgcg cagggggtcg gcgaagtgcg
     8401 cggcatctat gtgatgggca aagccgctac cctaaacgga cgggtcggtg acgtgatgat
     8461 ctcgtcggtg gtgcatgacg agcattcgct caacacctac cttttgcgca actgctttgt
     8521 gtccggagac gtgcagtcgt atctggaaca cggaacggtg ctcgacaacc agaaagccct
     8581 gacggtgcgc agcgcatttt tgcaaaaccg tgcctacatg gggagcttct atcacgaagg
     8641 ctacaccgtg ctggaaatgg aggcgggtcc gtatctgtct gcgatctatg agatcgtcaa
     8701 tccaagccgc catcccaaaa acgaaattgt ccacctgtca aacagcacgc cgtttgaagt
     8761 cggacttttg cattatgcgt ccgatacgcc atactctcgt cgacagagcc tgctgtcgaa
     8821 gagcctgagc tatttcgggg tggaaagcac ctacggctgt accatcgcga tactacagcg
     8881 aatactccgc agcgaaatgg ccaggcttgg gtagtgtccg gcaaagccac acctgcgaaa
     8941 tggacttgtc tgtcgttcga cggtggtgaa cactggaata atgcctgttc acgccgaagt
     9001 ccaagctttc cttcagatgc tcgcggcggc caatctgccg ccctatgaac attccaccgt
     9061 ggcgcaagca cgcgagcgca tgaacgccat cgcgatgctg gaacgaccgg caagtcccct
     9121 cgcggaagtc cgcgatctgt gcattccggc cccccacggc gacattcccc tgcgtctgta
     9181 tcgatccagt ttggaccagg cattgccgac gctgatttac tttcacggcg gcggctgggt
     9241 ggttgggagc atcgaatccc atgaccgact ctgccgcgcg ctggcaagtg cgtccggatt
     9301 tgcggtgatc tcggtggaat accgcttggc cccggaacac aagtatcccg ccgccgtcga
     9361 agatgccgaa gctgcgtacc gctacgtgcg cgcgtttgcc gacaagctgg gcgtggattc
     9421 cgaatccatc tttctgggag gcgacagcgc gggcggcaat ctatctgccg tcgtgtcgca
     9481 aagggcccgt gatgccggtg aaccactgcc gcgcggacaa gtgctcatct atcccgcgac
     9541 cggatctgcc gccgtcacag agtcgcgcaa ggagtttgcc gacggctatc ttctgaccgc
     9601 gggcgcgatg atgtggttta ccgcgcacta catttcggat cttgcacagc ttcgggaaag
     9661 cagcctgtcg ccctgctttg ccaaagacgt atcagggctg ccaccgacat tccttttgac
     9721 cgccgagttt gatccgctgc gggatgaagg ggaagcctac gcagccaagc tcaccaaagc
     9781 cggagtcaac gtgacgctgc gccgctacga cggaatgatc cacggatttt tgcagcttag
     9841 cgccatggtc agcgccgcaa cgaccgcgat cgaagacatc gcaacctggc tcaagcaaac
     9901 gcagcgaggt acatgatgac cacttggggg gacgatgtct tggccaccga atcaccgccc
     9961 tttgcgggtc gattccgttt gcagcgccag ctcggatcag gagggatggc cgatgtcttt
    10021 ctgtcattcg atgaagagac gcagaccccc tgcgcgatca agctcctttc gcagcactcc
    10081 gatccggaac agagacagcg cttttttcgg gaagcctctg tcctttcgca gcttcagcat
    10141 cctgggatcg tccgttacca cgcgcatggt tacaccgaac agggtgtgcc ctatctggcg
    10201 atggaactgc tggaaggaga agacctctgc cagcgacttc tgcgcaaacc gctgaccgcc
    10261 gcagaaaccc tggtgctggc gcaagccgtc ctgtccgccc tgacagccgc ccaccaagcc
    10321 gggatcatcc atcgggacat aaagccggcg aatttgtttt tggtgagtgg ggagcctgcg
    10381 cgcaccaagc tgctcgattt tgggattgtg cggctgacca cagcaagcca gcacacccgc
    10441 actggatcga ttctggggac gccggaatat atgtctccgg aacagctccg ggggctgaag
    10501 gaattggacg cgcgatccga tcttttttct ctcggtgtcg tgctgtatga atgcctggct
    10561 ggatggtcgc cctttcgcag cgatcacccg atgggaacgc tcggtagaat tctgtttgat
    10621 gatccaccca agctcagcga gcgaaacccg catctgccag aagcactctg tgtactcgtc
    10681 catgaaatgc tcgcgaagga accgcgacac cgtcccccaa gtgccgaagc ggttcgcaca
    10741 cgtcttcaaa acatatcggt cgattccgat ggcgacagaa tcaccggctg gcgcgcacct
    10801 gcggccctga cccaccgcga gctgcgctgg ctgtcgatga tcattttcca gccgacccgg
    10861 atcgatctgg cgctagaaaa aacatcgatt cttgaagcgg gcgaactgga tcaagacgcg
    10921 attggacaga cagcagcggc acactctgca cgccgagaaa agctagccga tggcacccag
    10981 attctgcttc tggatggaaa cgccaccgcc ggtgaacaag ccgagcaagc ggcgctgtgc
    11041 gcgatcgagc tgcattccca tgatccacac acagccatcg ccatttgttt tggacagacc
    11101 gatctgggcc agcaccgctt ggatgcccaa gcgctagagc gcgccgccga catgctcgat
    11161 gcatggcgaa agcgcacggc ggcggcaagc acacaaaagg aacgatccag cgcgcccatc
    11221 ttggtggatg aatcgctgcg gcgcatgctg atgtcgcgct ttgaatttgt agagcaagac
    11281 agtgcgtggg aactcactcc caccgagcgg cgcgacgagc tgcacgtact cggtcgggtg
    11341 tccccctttg ctggacgaga caccgagctt acacagcttg ccactgcgct ttctgcctgt
    11401 tgtgaagatt cccaagccgc tgcggtactc atcaccgcag agcccggaat gggaaagtcg
    11461 cgtctgtgtc atgaattcct gcgccaagcc aagctgtcct atcccgatct cgaagtttgg
    11521 atcggagccg gagatcccag cagcgcaggg tctccgtttc atctgatcgc gcaagccatc
    11581 cgccaagcga tgggcgtgca aaagagcatg acccaaacag aaaaaacgga gcggctccac
    11641 agcttcgtaa gcggacgctg taacacagca gatcacgaac gcatcggcga atttttggga
    11701 gagctgctcg gcatcacgta ccaacaaacg ccatctgtga ccctccaatc ggcgcgcagc
    11761 aatcccgtgc tgatgggtga tcagattcgt tttgcttttc ttgatctgct gcgtgccagc
    11821 atggcagacc atcccctctt gctcttgcta gaggatctgc actggggcga tttgccgacg
    11881 gtccagcttg tcgaggcggc gctccgcaac cactccgaaa gcccgctgtt tttgctcgcg
    11941 ctggcccgtc cgcaggtcga cgatctgttt cccaagctgt gggacagagc gcgacttctg
    12001 aagctgccgc taagaccact ttccaagcgc gcctgcgaaa aaatcgccaa aggaatcttg
    12061 ggcaatcggt ttggcgcaga caagctcacc aagctggccg agcgcgccgg aggacatccc
    12121 tttttcctcg aagagctgct gcgggcgaca tcggagagcg attcctttga actgcccgaa
    12181 agcgtccttg ccatggtgca gtcgcggatc ttgcgactcg attcgatggc ccgccgggtg
    12241 ctgcgcgccg gcagtgtgtt tggccgggtg ttccatcgcg gaggtctcac caagatgctc
    12301 ggaatcgacc attccgtagg gtcttcggaa tcggaacaga agtcgctccg cgatgcgctg
    12361 tccacgctgg ttgataggga gtttgtgaat ccgcggcacc ccagtcgctt tgccggtgaa
    12421 gaagagtttg tcttttcgca cgcgctggtt caggaatcag cgtactccat gctcacggcg
    12481 gaagatcggc agcttggtca tcggcttgca gcggaatttc tgcaagcggt cggggaagac
    12541 gatgcgatgg tgctggcgga acactgcacg ctcggcggcg tcttggacca agcggtgatg
    12601 tacctcaggc aagttgtcag cgaagccttg actggcaatg acttccgcgc cgcactttcc
    12661 tgcacagcac gcgcgctcaa gcttggggcc gaaggaaccg tgcgaggaac gctgctcgcg
    12721 atggaagccg aagcccacca gtggcttggg gaatttcgcg ccgcgataga cagtgccgaa
    12781 gaagccctgc atcttctgcc agtcggttcc gtaccttggt atcgcgccgt cgatgccctc
    12841 tccattggct gcggacgact gctcctccac gatcgcttga gcgagctcgc agtacaactt
    12901 ctcgcactgc tccatcacca aacagagtcg gaacatttct cgctgccggt tcttgcatcc
    12961 agcgcggtgc gcttgggaat gcagctctac atctgtggac ggtacaagga cgcgctggcg
    13021 ctactgcaac ccatcgaagc gctgattgac gccgatccaa atcccaagcc ccccgcgctg
    13081 ctcgcgtcca tttgcgtgct gcgcgccaat cgcgccgcgt atgaatggaa aattgatctg
    13141 tgtcccgact actttgaaca gtccgccaat ctgctcgatc aaatcggcga tcggcgcgcg
    13201 gcctgttcgc agcgactcga caccgcgctg ttctgttccg acatcggtga ctttgttcgc
    13261 atggaacggc tgctccgcga agtcattccg gttgctttga cgctcaacct gctgcgcgtg
    13321 ctgggcgtct ccttttccgc gctcggcgtg gcaaggtggc gacagggaca tcatgaagaa
    13381 gcgctgaccc actgccaaaa agcgctcgcc cagcttgaat cactggggga tgcgcggcaa
    13441 gaaggcgtct gtcgcataaa ccttgcccgc atcctctatt cactgggaca gtttgaaaaa
    13501 gcccatgccg ccgcgctgac ggccagtgaa cggctcgcgg aaattccccg gtttcgcgtg
    13561 cgcgcccttg catggctggc ccggattgaa cagcagcgcg ggaacctcga cgccgcggca
    13621 cttgctgccg aaacaagt
//
LOCUS       DAORGN010000135        13607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1870_length_13607_cov_6.076889, whole genome shotgun sequence.
ACCESSION   DAORGN010000135 DAORGN010000000
VERSION     DAORGN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_13607_cov_6.076889"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PKL17_14410"
     CDS             <1..1606
                     /locus_tag="PKL17_14410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNK45977.1"
                     /translation="LFGILIAAFIVSFGPGSPTGDSVFDGLGGRYAARVHGKDISEPE
                     LNFAFIALGLPNRGEAQAQKLREVVIDRLIERELLAHEAEQAGMLVSEDEAAKLLVDG
                     NMFVAGIARKVEPYAMRNNVLDYDRLRMVVQNSYRLTMKQFVDIQRRELLADKFRQLL
                     RAGTRASLDEIRGEYEDKNRQTNLEYVRFAPYRFEQELVASDADIEAWATAHEADIKK
                     TYEERKLLYTKQEKSAKLRRILIELKKDASDQQVNEAKAKLTAALQTITGGKSFAEIA
                     MATSEDDATKKRGGSLGYRKKTTTDLGADLENKIFSAKEGEIIGPERGERGLELVKVE
                     SFREGDIPIEKARAELAEELYRAAKGKELAQTSAADAVAKLKAGGKLAELYPKDDSDA
                     AEAQAKGSKIQVEETGMFARRGDTVPGIGSSGPLVKMAFTLRPGEVIGPLEISGSFVV
                     ATLKERKEPDLAEFEKKKDELSAEYGRTKWARLVTEYARHACVAANADGKLKVNATII
                     SDEPPARRGAAPALAASKYEPCKDRF"
     gene            complement(1715..3427)
                     /locus_tag="PKL17_14415"
     CDS             complement(1715..3427)
                     /locus_tag="PKL17_14415"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNK45978.1"
                     /translation="MKHTLAIRLGLFGALSLLIGGCHGGKTKAPPLPATAAAPTQSHA
                     IELLVATRMWNTTEPCGCTSTPLGDVARVAGLLQAAPGRSLLLDAGGLRYEPKSLPAT
                     RLAQARLKADFIEQTWRDLSAVTMVQPEDLRGRDGVAELSQGQRLVANLSGLPDGTTT
                     AQVVRTVLGQKLGIVGLADPDAPWPAGITVSNPAAALTQQLDSLGKEGVKAVVVLAGM
                     PRDKVRRLARKFPSVKLWVAGADDNVPDGAELPEQLDGAILVAPGHKGERLYRILLHP
                     TAQGTLMWRLQLTDKQRQRQQNILQQRIQHAETLLVELQKDAAADPSFVKTRQDELTS
                     LRNEQAQLARPSDDKDGFVTAELLPIAKTLPRDPNVRKKLDELDQQIGQANLQALSGP
                     PPEALPGSPRYVGNTACLGACHFHEAAHKLWQTTQHSRAYRTLVDVGKQRSYDCVNCH
                     STGFENPAGCNIMTLDKWQQANTPPVGVGPDLRHVGCEVCHGPGSLHIANPGKTKIPQ
                     PRPSASTCLDCHTPEHSDTFEWKAYARGILGEGHGAATRESLGPGPTGRELRQAARKQ
                     FESH"
     gene            complement(3446..3622)
                     /locus_tag="PKL17_14420"
     CDS             complement(3446..3622)
                     /locus_tag="PKL17_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNK45979.1"
                     /translation="MISPELLSILRCPKCKGVLREGQAQLTCSACKLVFSVDDGIPNM
                     LIEDARPIAGAEAQ"
     gene            complement(3665..4753)
                     /locus_tag="PKL17_14425"
     CDS             complement(3665..4753)
                     /locus_tag="PKL17_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK45980.1"
                     /translation="MNSAALASQAQPPRSATEEIWRRNRQQLVDDMLAGKTQLLGKPV
                     HLKLELTNYCNLSCPMCPHQSMQRPVGYMSPALFRRIISQAVPELEFAYVHHLGESLF
                     HGRIGELIAYGARAGVSMGLSTNATFLDERKALALLDSGLRFLVISLDANSPQSYEKM
                     RSGGEFQKTVRNIERLLELRPLRSPSLHVVVQLIISAYNQHEVSEFARRWPNEVVIKE
                     ARDWAGQVSLSSLLPRSRALGQSFPLERPQVLPMDAPSLQSPCRLPFGELTVLWDGKV
                     VPCANVFEHVNLLGDLSVQSLDEVWNGAPIMALRQAHHRKKVAAIPVCRSCAGHALDP
                     TDFVSVDQLSQRLRNYQGGQLSPRSGLS"
     gene            complement(4746..7520)
                     /locus_tag="PKL17_14430"
     CDS             complement(4746..7520)
                     /locus_tag="PKL17_14430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNK45981.1"
                     /translation="MPRWLTLLTFWLPTLVGTAVFAGVTTPPGKATPALQTKMDREVV
                     REGEVEGVQAYRLKNGLRVLLIPEPSASSITIEVAYSVGSRHEGYGEAGMAHLLEHLL
                     FKGTARFPHPDEEIDRRGGEWNAETEVDQTRYYQTQLPSDDNLRVALSIEADRMTQVR
                     IRPSDLAKEFQVVRNELELADSEPRDILLRRIHHAAFAVHSYGRDPIGTRSDIERVSV
                     ARLQAFYARYYRPDNAHLVIAGPIAVDKTLTLVRDLFGPIRPVGPPPERTYTEEPAQD
                     GERQVTVRRVGKLPLIGMYFHSASVADPDQPALLALADILARPGSGRLYKALVDTGLA
                     VSVAADSELRAEPGGLLLWAELLRGGDLQKVQQTMTTIVEDVVQKGITADELTRFQVR
                     ALAQHQRLARSPAELAHTLGIFATLGDFRLLFLLRDRTQKLDVATVQQAARRYFLRQN
                     RTVAHFVPEELPPLKHARPTVDVTTALSGFQAGGAIQPGEPFVQKPATIAQRVITRQL
                     PSGMKLVFVPKRTRGQHVELSLSLSVGNPDDLKGREAALSLLGPLLLRGSKLHDYQQL
                     LDELDLHKATLQLANEAGLPGLTSQQIELSASTVRSELPALLSLLHEVVTAPGLRPEQ
                     FERVRQQALAQSEDLTHNPMAQALTKLLRQAAPYPPTDPRYIPTTSEQLARLTAAKLD
                     DVRFLASELLGGQNAQLVVIGDIDPEAVLAQVQTLFGRFVARRPYVRLPRPHQKVAAS
                     DLMVQLPDRDSSAVAMLLPFQLTDASPDYPALLLWQQIFGAATTSRLNQRLREKDGLS
                     YEVRSSLRIPTQDDGASLAALATCAPQNSKAALSAMNEELLQLVEKGPSEAELAQAKQ
                     AYDKQLEAMLADDDDLALLLAHLSRVSRDIGYIDELRNQVHRVELPQLLSAAKRYLES
                     APRLRALTHE"
     gene            7636..9672
                     /locus_tag="PKL17_14435"
     CDS             7636..9672
                     /locus_tag="PKL17_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45982.1"
                     /translation="MLTDNISFLPKPSTSDISTRRQRRLLLRAASYRALLLGALLGGA
                     GCWEVPVTLAPTDAQGNTDGGSTDMSGGGDGGPAASGWHWESPIPQGNNLRALWGSAG
                     KSADQDDLFMGGEAGTLVVGRTTGWKVQRSPGNGSRTILGMSGYLASGIPQVVAVGMY
                     DLSLLYNGSAWSDVSPFIGTGDGQLTAVWASPTAGEFFSVGTTGRLYRVTRANNLLSY
                     TPEAQGVTTDSLFAVSGVPGATAPDVYAVGANGRILHRGADGKWLIEANGMAAQQLNA
                     VWVGTGALAGDVYAAGDSGIIMRKSAGNWSFERPPTTANLTALWGAGDELYAAGAKGT
                     LLRRKAGAWQLEAVGLTSELISALWGTDRGTATNTVYAVGNLGTILRRERDTWSQVSS
                     RVTQTALASVWAASPSEAYAVGTDGLILKHSGVGAAGTWKIVPTASMTTLNAVNGYVP
                     AVGDSDVWAVGTEGTILHQAGSVWVIEGGVLGVAELTGVWVGADAVVVVGRSGRIYSK
                     IQGNWNIEPGPMGAPITDDLFSAWGVGTGDSRVVYAAGAKGIILRKDKNGWTQEAAGL
                     SSETLVSLVGNAEEGLTALGNKGSILRRSGGKWTTSSARPLTQTGATGIAACTVAKTG
                     DVWGVGTQGVIMKRTVSDWVSETSLTAQPFTGIGCASPDDIFVVGPGGLILHKY"
     gene            complement(9726..11156)
                     /locus_tag="PKL17_14440"
     CDS             complement(9726..11156)
                     /locus_tag="PKL17_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK45983.1"
                     /translation="MDIQTSNQESLDADVIIVGGGVTGCAMAVALANGKRRILVLEAR
                     LGHKPRFAGELLHPTGTQVLDDLGFLPTLRHHGGVDVDGFAVVKAPKVPVTRLPYGEI
                     ENNRPTGFAMEHRDLVTGMREWAQKQPGITFRFNEKVEEILRNDRGRVCGVRTASGTL
                     HANLVLGCDGRHSKIRPLLGLSENARLLSFSVAMRLPDGAKLLPCPTFGHIFLGAWGP
                     MLIYPIGGPDGSRDARGCFDVTRELSGGPKGAVELLKREYAPHLPEPLAKALIESLDR
                     ELPQIAANEAIRTDQAVLPGAAIVGDAGGCAHPLTAAGMTVCFTDAQRLGRLLRHAGD
                     FSDMRLVDTALRQYEIDRYRFVRVRELLTDALYEVFRGAEPGTRAIREGIFRYWESSK
                     VARARSMALLSGADSRPAVFLKEYLTVVGTSTSAVLAGETGPVRGETATIKQRFDSFL
                     GLGQKSFEKARLVLRDVRQEFLRASA"
     gene            11444..12283
                     /locus_tag="PKL17_14445"
     CDS             11444..12283
                     /locus_tag="PKL17_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45984.1"
                     /translation="MAKPDFCKQASARRAIRDGQRVFGAVVLALLSASPAWADATDGN
                     AAQPAASQPSDAEMTAAKQHFDTGAQFFKTENYAAARAEFQAAYDLSKLTPLLYNLGR
                     AAEGQGQKADALRFYEQYLATNPSDAAEVRTRVANLKTDTGSAATAPAPLDGGPAWLK
                     GTAKLPPIPAIAALGAGVAFVAIGIGTGVAAKSVEAEVGTPSNRYWTGDAAALYDKGK
                     ALNGTAIAFGVLGGVALAGGAAWTGYWFYLRGKQPSATTPTAAPTARIIPTGNGLAVV
                     GGF"
     gene            12427..12951
                     /locus_tag="PKL17_14450"
     CDS             12427..12951
                     /locus_tag="PKL17_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45985.1"
                     /translation="MSGRCQIGNGGTSTGDGGASGPSGCADGMGSEVSGSSAGKPAWA
                     CAGTYQASSDPAKNATKLCAAGYDICTVADSINLNTCNNLGGFYLANVPVRWQFTTPS
                     CGPSGGQGNQTAGFAGCGKSVNSVFGISSCSGFSKAMSDWQNSTLQISSPFSPLTTTT
                     SSSASSNGVLCCQK"
     gene            12994..>13607
                     /locus_tag="PKL17_14455"
     CDS             12994..>13607
                     /locus_tag="PKL17_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNK45986.1"
                     /translation="METLTTAAPQTEAVHPVLSKPLDPADVPVLRDAYRQMLLIRRVE
                     EAAAKAYGQGKIGGFLHLVIGQESVCVGAALALQPQDYMIASYREHGHFMAKAGSTLE
                     SAKAVLAELQAKDTGCSKGLGGSMHMFSAAHRFLGGYGIVGGQAPLAAGVAFASKYQN
                     LPEVTLCFLGDGAVNQGAFFEGLSLAGLWKLPVVFIVENNQYSMG"
BASE COUNT         2620 a         4076 c         4308 g         2603 t
ORIGIN      
        1 cctcttcggc atcctgattg ccgccttcat cgtcagcttt ggccccggtt cgccgacggg
       61 tgattcggtg ttcgatgggc tgggcggtcg ctacgcggcg cgcgttcacg gcaaagacat
      121 cagcgaaccg gagctgaact tcgccttcat tgcgctcggg ctgccgaacc gcggcgaggc
      181 gcaagcgcag aagctgcgcg aagtcgtgat cgatcgtctg atcgagcgtg agcttctggc
      241 ccacgaagcc gagcaagcgg gcatgctggt gtccgaagat gaagcggcga agctgctcgt
      301 cgacgggaac atgttcgtcg ctggcatcgc gcgcaaggtc gaaccgtatg cgatgcgcaa
      361 caacgtgctc gattacgatc ggctgcgcat ggtggtgcag aactcgtatc gactgacgat
      421 gaagcagttt gtcgacatcc agcgccgcga gctgctcgcc gacaagtttc gccagcttct
      481 gcgggcggga acgcgcgcct cgctcgacga aattcgcggt gagtatgagg acaaaaaccg
      541 ccagacgaac ctcgagtatg tgcggtttgc accgtatcgt ttcgagcaag aactggttgc
      601 gagcgacgcc gacatcgaag cctgggcgac ggcgcacgaa gcggacatca agaagaccta
      661 cgaagagcgc aagctgcttt acaccaagca ggaaaagagc gcgaagctgc gtcgcatcct
      721 catcgagctg aaaaaagacg ccagtgacca gcaagtaaac gaagccaaag ccaagctgac
      781 ggcggcgctt cagaccatca ccggcggcaa gtcgtttgcg gaaatcgcga tggcgacgtc
      841 tgaagacgat gccaccaaaa aacgcggcgg cagcctcggc tatcgcaaaa agacgacgac
      901 ggatctgggc gccgatctgg aaaacaaaat cttttcggca aaagagggcg agatcatcgg
      961 accagagcgc ggtgaacgcg gtctggaact tgtgaaggtc gagtcgttcc gcgaagggga
     1021 catcccgatt gaaaaagcgc gggcagagct ggctgaagaa ctgtatcggg cggccaaagg
     1081 caaagagctg gcgcagacga gcgcagcgga tgcggtagcg aagcttaaag ctggcggcaa
     1141 gctggcggag ctttatccca aggatgattc cgacgccgca gaagcccaag ccaagggcag
     1201 caagatccag gtcgaagaga ccggcatgtt tgcgcgccgc ggggacaccg tgccggggat
     1261 tggttcgtcg gggccgcttg tgaaaatggc gtttacgctg cggcctggtg aagtgattgg
     1321 cccgcttgag atcagcggtt cattcgttgt cgcgacgctc aaagagcgga aagagccaga
     1381 cctcgcggag ttcgaaaaga aaaaggacga gctgtcggcg gaatatggtc gaaccaagtg
     1441 ggcgcggctc gtcaccgagt atgcccgcca tgcctgtgtc gcagccaatg ccgacggcaa
     1501 gctcaaggtc aacgcgacga tcatttccga tgagccgccg gctcgtcgtg gtgccgctcc
     1561 tgcactggcc gcgtcgaagt acgagccctg caaagaccgc ttctagtcgt tttcacgccg
     1621 ctgggtcgaa gcgcccgctt cgcaccttcc ccatcaccca tacgtagcgt cgtccgctgg
     1681 gttttcgccc atgcggcgtc ggtgtgctgt tgtgttagtg cgactcgaac tgcttgcggg
     1741 cggcttgacg aagctctcgt ccggttggtc cggggcccag gctttcgcgg gtggctgcgc
     1801 catgtccttc gccgagaatt ccacgagcgt aggctttcca ctcgaaggta tcggagtgtt
     1861 cgggcgtgtg gcagtcaaga caggtgctgg cgctgggacg cggctgcggg atcttggtct
     1921 tgccgggatt ggcgatgtgc agcgatcccg gaccgtgaca gacttcgcag ccgacgtgcc
     1981 gcagatcggg accgacaccg acgggcggtg tgtttgcctg ttgccacttg tcgagcgtca
     2041 tgatgttgca gcccgccgga ttttcaaagc ccgtcgaatg acagttcaca cagtcgtagg
     2101 atcgctgctt gccgacgtca acgagcgttc gataggcacg gctgtgctgg gtggtttgcc
     2161 acagcttgtg ggccgcttcg tggaaatgac aggcgccaag gcatgccgtg ttgccgacgt
     2221 agcgcggact gcccggcagt gcctcgggcg gcggacccga cagcgcttgc aagttggctt
     2281 gaccgatctg ctggtccagc tcgtcgagct ttttgcgcac gttcggatct cgcggcaagg
     2341 tcttggcaat cggcaaaagt tcagccgtca caaacccgtc tttgtcgtcg ctgggccgcg
     2401 ctagctgcgc ctgttcgttg cgcaggcttg tcagctcgtc ctgtcgcgtc ttcacaaacg
     2461 agggatcggc ggcggcgtct ttttgcagct ccacaagcag cgtttcagcg tgctggattc
     2521 gctgttgcag gatgttttgc tgtctctgcc gctgcttgtc ggtaagttgc agccgccaca
     2581 tcagcgtgcc ttgtgccgtc ggatgcagca ggatgcgata gagccgttcg cctttgtggc
     2641 ccggcgccac caggatcgct ccgtcgagct gttcgggcag ctcggcgccg tcgggaacat
     2701 tgtcatcggc ccccgcgacc cacagcttga ccgaaggaaa tttgcgcgcc agccgacgta
     2761 ctttgtcgcg cggcattccc gccagcacca ccaccgcctt gacaccttct ttgcccagcg
     2821 aatccagctg ctgcgtcagc gctgcggcgg gattgctgac ggtgattccg gctggccacg
     2881 gcgcatccgg atcggccaaa ccgacaatcc cgagcttttg cccgagcacg gttcgcacca
     2941 cttgcgcagt cgtcgtgccg tcgggaagtc cgctgaggtt ggcgaccagt cgttggcctt
     3001 gcgaaagctc ggcgacgccg tctctgccgc gcaggtcctc gggctgaacc atcgtcaccg
     3061 ccgacagatc gcgccaggtc tgttcgataa agtccgcttt gagccgcgct tgcgcaagcc
     3121 gggtcgcagg cagcgacttt ggttcgtagc gcaagcctcc cgcgtcgagc agcaagcttc
     3181 gtccgggagc agcttgcaga agaccggcga ctcgcgccac atcaccgagc ggtgtcgacg
     3241 tgcagccgca cggctcggtc gtgttccaca tccgcgtcgc gaccaacagc tcgatcgcat
     3301 ggctttgcgt cggtgccgcc gctgtcgcag gaagtggtgg cgctttggtt ttgccgccat
     3361 gacacccgcc gatcagcagc gagagtgcgc caaaaagacc cagccggatt gctagcgtgt
     3421 gcttcaacgt cagagcctcg ctgctttact gtgcctcggc gccggcgatc gggcgagcgt
     3481 cttcgatcag catgttggga atgccatcgt cgacggaaaa aaccagcttg caagctgagc
     3541 aggtcaactg ggcttggcct tcgcgcagca cgcctttgca cttggggcag cggaggatgg
     3601 aaagcagctc ggggctaatc atgagtcgga acctttcttc ccgcctataa caaactcgac
     3661 gcggctaaga caagccagag cgcggcgaca gctggccgcc ttggtaattg cgcagccgct
     3721 gtgatagctg gtcgacggaa acaaaatcag ttggatcaag cgcatgtccg gcacaactgc
     3781 gacacactgg aatcgcggcg acctttttgc ggtgatgggc ctggcgcagc gccatgatcg
     3841 gtgcgccatt ccagacctca tcgagcgact gcaccgacag atcgccgagc agattgacgt
     3901 gctcaaacac gttggcgcac gggaccactt tgccgtccca caacaccgtc agctcgccaa
     3961 acggcagacg acagggcgac tggagggacg gcgcatccat cggcagcact tgcggacgct
     4021 cgagaggaaa cgactgcccg agcgcccgac tgcgtggcaa aagcgacgaa agcgacacct
     4081 gccccgccca gtcgcgcgcc tctttgatca ccacttcgtt tggccagcga cgggcaaatt
     4141 cgctgacttc gtgctggttg tacgccgaaa taatgagctg cacgaccaca tgcagcgacg
     4201 gactgcgcaa aggccggagc tccagcaggc gttcgatgtt gcgtaccgtt ttttgaaact
     4261 cgccgccgct gcgcattttt tcgtagctct gcggtgagtt ggcgtcgagc gagatcacca
     4321 aaaaacgcag ccccgaatcg agcagcgcca gcgcttttct ttcatccaga aaggtcgcgt
     4381 tggtcgacag ccccatcgac acgccggccc gcgcgccgta agcaatcagc tcgccgatgc
     4441 gcccgtggaa cagcgattcc cccaggtgat gtacataggc aaactccagc tcgggcaccg
     4501 cttgcgagat gatccgacga aacagcgctg ggctcatgta tccgacgggg cgctgcatcg
     4561 actgatgcgg gcacatcggg cagcttaggt tgcaatagtt ggtaagttcc agcttcaggt
     4621 ggaccggctt ccccaaaagc tgcgtctttc cggccagcat gtcgtcgacg agctgctgac
     4681 ggttgcggcg ccatatctct tctgtggcgg accgcggcgg ctgtgcctgg ctggccaagg
     4741 ctgcgctatt catgcgtaag agcccgcaga cgcggcgccg attccaggta acgcttcgcc
     4801 gccgagagaa gctgcggcag ctcgacgcga tggacttggt tgcgcagctc gtcgatgtag
     4861 ccgatgtcgc gcgacacccg cgaaaggtgt gccaaaagca acgccaggtc gtcatcatcg
     4921 gcgagcatcg cctccagctg cttgtcgtag gcttgtttcg cttgcgctag ctcggcttct
     4981 gacggccctt tttcgacaag ctgcaaaagt tcttcgttca tggccgaaag cgccgctttg
     5041 ctgttctgcg gggcacacgt cgccagcgcc gccaggctcg cgccatcgtc ttgcgttgga
     5101 atgcgcagcg acgaacggac ttcataagac agcccatctt tttcgcgcag cctctggttc
     5161 agccgagacg tcgtcgctgc tccgaagatt tgttgccaca aaagcagcgc cggataatcg
     5221 gggcttgcat cggtgagctg gaacggcaag agcatcgcca ccgccgagct atcacgatcg
     5281 ggtagctgca ccatcagatc gctcgccgcc actttttgat ggggccgtgg cagacgcaca
     5341 tacgggcgtc gcgccacaaa tcgcccgaac agcgtctgta cttgggccag taccgcttcc
     5401 ggatcgatgt caccgatcac caccagctgc gcattttgtc cgccgagcag ctcactcgcc
     5461 aaaaagcgca catcgtcgag ctttgcggcg gtaaggcggg caagctgctc gctcgtcgtc
     5521 ggaatgtagc gagggtcggt cggtggatac ggtgccgctt ggcgcaacag cttggtcagc
     5581 gcttgcgcca tcgggttgtg cgtcaggtct tcgctctgcg ccagcgcttg ctgccgcacc
     5641 cgctcaaact gttcgggacg cagacccggc gcggtcacaa cttcgtgcaa aagcgacagc
     5701 agcgctggca gctcactgcg caccgtgctt gctgacagtt cgatctgctg gcttgtcaga
     5761 ccgggcagac ccgcttcatt cgccagctgt agcgtcgctt tgtgcagatc cagctcgtcg
     5821 agaagctgct gataatcgtg aagtttgctg ccgcgcaaaa gcagggggcc aagcagcgac
     5881 aatgccgcct cgcgaccttt gagatcatcg ggattgccga cggacagcga cagcgacagc
     5941 tcgacatgct ggccacgggt ccgcttgggg acaaagacaa gcttcatgcc gctcggcagt
     6001 tgccgggtta tgacgcgctg cgcaatcgtc gctggctttt gcacaaacgg ctcgcccggc
     6061 tggatcgcgc cacccgcttg aaagccagaa agcgccgttg tcacgtcgac agtgggccgc
     6121 gcgtgcttga gcggtggcag ctcttcgggc acaaagtgtg cgacggttcg attttgacgc
     6181 aaaaagtaac gccgagcggc ctgctggacc gtggcgacat ccagcttttg cgttcgatcg
     6241 cgtagcagaa acaacaaacg gaagtccccg agcgtggcaa agatgcccaa agtgtgtgca
     6301 agctcggcgg gactccgcgc cagccgctga tgttgggcca gggcgcgaac ctgaaagcgc
     6361 gtaagctcgt cggcggtgat gcctttttgc accacatctt cgacgatggt ggtcatggtc
     6421 tgctggactt tttgcagatc gccgccacgc agcagctcgg cccacaaaag cagtccgccc
     6481 ggctcggccc gcagctcgct atcggcagcg acgctcaccg caagtccagt atcgacgagc
     6541 gctttgtaaa gtcgtccgct cccaggccgc gccaagatat cggccagcgc gagcagggcc
     6601 ggctgatccg gatcagcgac cgacgccgaa tggaaataca tcccgatgag cggcagcttg
     6661 ccgacgcgac gaaccgtgac ttggcgttca ccgtcttgcg ccggctcttc ggtataggtt
     6721 cgctcgggcg gcggaccgac gggccgaatc ggtccaaaca gatcgcgcac cagcgtcagg
     6781 gtcttgtcga cagcgatagg tccagcgatg accagatgcg cattgtcagg tcggtaatag
     6841 cgcgcataaa acgcctgcaa acgggcgacg gacactcgct cgatgtcgct gcgggtgcca
     6901 atcggatcgc gcccatagct gtggaccgca aacgccgcat ggtgaatccg tcgcaaaagg
     6961 atgtcgcgcg gctcgctgtc ggccagttcc agctcgttgc ggacgacctg gaattctttg
     7021 gcgagatcgg acggacggat gcgcacttgg gtcatccgat cggcttcaat cgacagcgca
     7081 acccgcagat tgtcgtcgct gggtagctgc gtttggtaat agcgggtctg gtcgacttcg
     7141 gtttccgcgt tccattcgcc gccgcgacga tcgatttctt catcgggatg cgggaatcgc
     7201 gcagtgcctt tgaacagcaa atgttcgagc aggtgcgcca tgcccgcttc gccgtaacct
     7261 tcgtgacgcg atccgacgga atacgcgacc tcgatggtga tcgatgacgc cgatggctcg
     7321 ggaattagta gcacgcgcag accatttttc agtctgtacg cctgcacacc ttccacttcg
     7381 ccttcccgca cgacctcgcg atccattttt gtctgcaagg ccggcgtcgc tttgccggga
     7441 ggggtcgtca cacccgcgaa aaccgcagtt ccaacgagcg taggaagcca aaacgtaagc
     7501 agagtcagcc aacgaggcat tgccgcaatt tagtacacat tgggtaccaa tgttcccacc
     7561 ttggcggtcc atctcggcga gaaagttcgc aacgtctcgc ttgacagacg actgcggctc
     7621 gcaaacgatc cgaatatgct gaccgacaat atttcgtttt tgccaaaacc aagcacttct
     7681 gacatttcga cccgacgaca gcgacggctt cttttacgcg ctgcgtcgta ccgggctctg
     7741 cttttgggcg cgcttctggg cggcgccggc tgctgggaag tgccagtaac gcttgcaccg
     7801 acggacgccc agggcaacac ggacggtggc tcaaccgaca tgagcggcgg cggcgacggt
     7861 ggtccagcag caagcggctg gcactgggaa agcccgatcc cgcaaggcaa caacctgcgg
     7921 gcgctgtggg gatcggcggg caagagcgcc gatcaagacg acttgttcat gggtggcgag
     7981 gccggcacgc tcgtcgtcgg tcgcaccacg ggctggaaag tccagcgcag ccccggcaac
     8041 ggcagtcgca ccatcctcgg catgagcggc tacctcgcga gcggcattcc gcaagtcgtt
     8101 gccgtcggta tgtacgatct cagtttgctt tataacggca gcgcatggag cgatgtcagc
     8161 ccctttatcg gcacaggcga tggacagctg acggctgtgt gggccagtcc aaccgcgggt
     8221 gagtttttct ccgttggtac aacaggccgg ctgtatcgcg tgacccgcgc aaacaacctg
     8281 ctgagctata cccccgaagc gcagggtgtg acgaccgatt cgctgtttgc agtgtctggc
     8341 gtgccgggcg cgaccgctcc cgatgtctat gcagtgggag ccaacggtcg tattttgcac
     8401 cgcggcgccg acgggaaatg gctgatcgaa gcaaacggca tggcggcgca gcagctaaac
     8461 gccgtgtggg ttggcaccgg cgcgctcgcg ggggatgtct acgcagcggg cgacagcggg
     8521 atcattatgc gcaaatcggc gggcaactgg agctttgagc ggcctccaac gacggccaac
     8581 ctgacggctc tatggggcgc aggcgacgaa ctgtatgcgg cgggcgccaa aggcacgctg
     8641 ctgcgacgca aagccggtgc ttggcagctg gaagcggtcg gtctgacgag cgagctgatt
     8701 tcggcgctgt gggggaccga tcgcggcact gcgaccaaca ccgtctacgc cgtcggcaac
     8761 ctgggcacca ttttgcgacg ggaacgcgac acctggagcc aagtgtcaag ccgcgtcacc
     8821 cagaccgcgc tggcatcggt ttgggctgcc agtccgagcg aagcctatgc ggtcggcacc
     8881 gatggcttga tcctaaaaca cagtggcgtc ggcgccgcgg gcacctggaa gatcgttccg
     8941 acggccagca tgacaacgct aaacgctgta aatggctatg tgcctgccgt cggtgattcc
     9001 gatgtgtggg ctgtcggcac cgaaggcacc atcttgcatc aagccggttc ggtatgggtc
     9061 atcgaaggcg gcgttcttgg cgtcgcggag ctgaccggcg tgtgggttgg cgcggatgca
     9121 gtcgttgtcg tcggtcgcag tggtcgcatt tacagcaaga ttcagggcaa ctggaacatt
     9181 gagcccggcc cgatgggcgc gcccatcacc gacgatctgt tttccgcatg gggcgttggc
     9241 acgggcgaca gccgcgtggt ctacgccgcg ggcgccaaag gcatcatcct gcgcaaagac
     9301 aaaaacggct ggacccagga agctgccggt ctgagcagtg agacgctggt ttctctcgtc
     9361 ggaaatgccg aagaagggct gaccgcactg ggcaacaagg ggagcatcct tcggcgatcg
     9421 ggcggcaagt ggacgacgtc ttcggcacgg cctttgaccc agaccggcgc gacaggcatt
     9481 gcggcctgca ccgtggccaa gactggcgat gtgtggggcg ttggaacgca gggcgtgatc
     9541 atgaagcgca ccgtgagcga ctgggtttcc gaaacgtcac tcaccgcgca gccgttcacg
     9601 ggcattggct gcgccagccc agacgacatc ttcgtcgtcg gtccgggcgg cctgatcctg
     9661 cacaaatact aacagcggtg attttggggg tatcggcggt cgtttttggg atgaccgccg
     9721 aacggtcagg cgctggcgcg caggaattcc tgtcgcacgt cacgtagcac gagacgcgct
     9781 ttttcgaagc tcttttgacc caggcccaag aacgaatcga agcgctgctt gatcgtcgcg
     9841 gtttcgccgc gcacgggacc ggtttccccg gccaaaacag cgctcgtcga tgttcccacg
     9901 acggtcagat attctttcag gaacaccgcg ggacgcgaat cggcacccga caaaagcgcc
     9961 atcgaacgag cccgcgccac tttgctgctt tcccaatagc ggaaaatgcc ttcccgaatc
    10021 gcccgcgtac ccggctccgc gccgcggaag acttcgtaca gcgcgtcggt caagagttca
    10081 cgaacccgga cgaagcgata gcggtcgatt tcgtactgac gcagcgcggt gtcgaccagt
    10141 cgcatgtcgg aaaaatcgcc cgcatgacgg agcagccggc ccagccgctg cgcatcggtg
    10201 aaacaaacgg tcatgccagc cgcggtgagt ggatgcgcac aacctcccgc atcaccgacg
    10261 atcgctgcac cgggcagaac cgcttggtcg gtgcgaatcg cttcgttggc ggcaatctgc
    10321 ggcagctctc ggtcgagcga ttcaatcagc gccttggcga gcggttcggg caggtgcggg
    10381 gcgtattcgc gcttgagcag ctcgaccgcg cctttcgggc cacctgacag ctcgcgggtg
    10441 acatcgaaac agccacgcgc atcgcgagag ccatcgggtc caccgatcgg ataaatcagc
    10501 atcggtcccc acgcgcccag gaaaatgtgc ccaaacgtcg gacagggcaa cagcttggct
    10561 ccatcgggaa gtcgcatcgc caccgaaaac gacagcagcc gcgcattttc gctcagcccg
    10621 agcagcggtc tgattttcga atgacgacca tcgcagccga gcaccaagtt ggcatgcagg
    10681 gttccacttg cggtgcggac gccgcagacg cggcctcgat cgttgcggag gatttcttcg
    10741 actttttcgt tgaaacggaa cgtgatgccg ggctgtttct gcgcccactc gcgcattccg
    10801 gtgacgaggt cgcggtgttc catcgcgaag cccgtcggac ggttgttttc aatttcgccg
    10861 tatggcagcc gagtgacggg aactttgggc gctttgacga cggcgaagcc atccacatcc
    10921 acaccgccgt ggtgacgcag cgtcggcaaa aatcccaagt catctagcac ttgggttccg
    10981 gtcgggtgta gcagctcgcc agcgaagcgg ggcttgtgtc cgagtcgtgc ctcgaggacc
    11041 aagatgcgtc gcttgccatt ggcgagcgcg actgccattg cgcagccggt tacgccacca
    11101 ccgacgatga ttacgtctgc atcaagggac tcttgattgc ttgtctggat atccatgcgc
    11161 gtatcctttg gctaagttgt tccgagcctg cgcacggggt cggcggcgag tcgaaagggg
    11221 tgcagttttg caccgtccgt ttcgccagtg tgctgggacg cacgggtttt tgtgctggga
    11281 cgcacggctt gtgccgacag aatgcatggc tcagacgaga agctgggttt ccagcggctc
    11341 tagccccagg gtcgaagcag tctttgtgcc aaagtgtggg ccgtcgggcg gcttgcggaa
    11401 aacccggccc caccttgcca gcttggcgtg ttatattcgc aacgtggcaa aacctgactt
    11461 ctgtaaacag gcgtctgctc gacgcgcgat acgtgacgga caacgggtgt ttggggcggt
    11521 ggtgctggcg cttttgtcgg ccagcccggc gtgggcagat gcaaccgacg ggaatgcagc
    11581 ccagcctgcg gccagccaac ccagtgatgc ggaaatgacc gccgccaagc agcacttcga
    11641 taccggcgcc cagtttttca aaaccgaaaa ctatgcggca gcgcgtgcgg agtttcaggc
    11701 ggcatacgac ttgtcgaagc tgacgcctct tctgtacaac cttggccgcg ccgccgaggg
    11761 acaagggcag aaggccgacg cacttcgctt ttatgagcag tacttggcga cgaatccgtc
    11821 tgacgctgcg gaagtcagga cgcgggtggc caatctcaag accgatacgg gaagtgcagc
    11881 gacggcgcca gcaccgcttg acggtggacc ggcatggcta aaggggaccg cgaagctgcc
    11941 acccatcccg gccatcgcgg cacttggggc aggcgtcgcg ttcgtcgcga tcggaatcgg
    12001 caccggcgtc gctgccaaat ccgtagaagc cgaggtcggt acgccgagca atcgctattg
    12061 gaccggcgac gcagcagcgc tatatgacaa aggaaaagca ctaaacggta cggctatcgc
    12121 gtttggtgtg ctcggtggtg tggcgttggc tggtggcgct gcatggacgg gatattggtt
    12181 ttatctgcgc ggcaaacagc caagcgcgac gaccccgact gcggccccga ctgcgcgaat
    12241 cattccgacg ggaaatggcc ttgctgtggt ggggggattt taacgaaagt aaccgcggga
    12301 gtttttacta tgcgtaaatt gctattcttt ccagttgctt tgttattcgg ttgttttgct
    12361 cccgacttga ctggggctat gtttctttgc gatcccagcc atttttgccc cgatggttat
    12421 tcttgtgtga gtggtaggtg tcaaatcggg aacggtggca cctcgacggg cgatggcggc
    12481 gcaagcggac ccagcggctg tgcggacggc atgggctccg aagtcagtgg ctcaagcgcg
    12541 ggcaagccag cgtgggcctg tgcgggcacc taccaagcgt cgagcgatcc ggcgaaaaac
    12601 gcgaccaagc tgtgcgcggc gggatacgac atttgcaccg tcgctgactc gatcaacttg
    12661 aacacctgca acaaccttgg tggtttttat ttggccaacg tgccggtgcg gtggcagttc
    12721 accacgccat cgtgcggacc gtcgggaggt caaggcaacc agaccgctgg ctttgccggc
    12781 tgcggcaaga gcgtcaactc ggtcttcggc atctcgtcgt gcagcggttt ttccaaggcg
    12841 atgtccgact ggcaaaacag cacccttcag atatcgtcgc cgtttagtcc gctcacgacc
    12901 acgacgagca gcagcgccag cagcaacggc gtgctgtgct gccagaagta aacccttcca
    12961 gccgcgcgcc ggcttgaaat atagtgctcg aatatggaga cactcactac cgcggctcct
    13021 cagacggaag ctgtgcaccc tgttctctcg aagccgcttg atccggcgga cgttccggtt
    13081 ctgcgtgatg cctatcggca gatgctgctg attcgtcgcg tcgaggaagc cgccgccaag
    13141 gcctacggac aaggcaaaat cggcggtttt ctccacttgg tgattggcca ggaatcagtc
    13201 tgcgtcggtg cggcgctcgc actacagccg caagactaca tgatcgcgtc gtaccgtgag
    13261 cacggccact tcatggccaa agccggcagt acgctcgaat cagccaaagc ggttctcgcc
    13321 gagctgcaag ccaaagacac cggctgctcg aagggtttgg gcggttcgat gcacatgttc
    13381 agcgccgcac atcgtttcct tggcggctat ggcatcgtcg gtggacaggc gccgctggcc
    13441 gctggcgtgg cgttcgcgag caagtaccaa aacctgcccg aagtgacact gtgctttctc
    13501 ggcgacggtg cggtgaacca gggcgcgttc tttgaaggtc tgtcgctcgc cggtctgtgg
    13561 aagctgccgg tggtgtttat cgtcgaaaac aaccagtatt cgatggg
//
LOCUS       DAORGN010000136        13598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1873_length_13598_cov_5.774422, whole genome shotgun sequence.
ACCESSION   DAORGN010000136 DAORGN010000000
VERSION     DAORGN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1873_length_13598_cov_5.774422"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="PKL17_14460"
     CDS             <1..503
                     /locus_tag="PKL17_14460"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HNK45987.1"
                     /translation="LRDLGRAAQFVEQLALRQKLLTMGMGLRQELLQDTLSRTVPDAS
                     QATWDDVLGTMLANHLLQRPAALVPIMRRDLVPLRRMIGPQDEPKQAQLPELLRARCT
                     KLIEERIADRQTDPESARLLLGPAQRAFVERVQSQLAQGLATLPDVLDDEAADVVPRV
                     PGLLLG"
     gene            complement(492..2033)
                     /locus_tag="PKL17_14465"
     CDS             complement(492..2033)
                     /locus_tag="PKL17_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45988.1"
                     /translation="MTTRKSDGDDTVPFVTGAVPVPIEEDNTPLPTRIPLGARPPPPP
                     LYEGKLLSLRSGSQLQLRSLAKLRLLRIDDLAEPGDDKRWLSPSSRPPRAVVRRDAEL
                     RTLQRDPVTIPAPASVSADALVPLAQKELVPHETEPRPTFRWLRLAPPGGSQTDISAA
                     QLCGWAVATGYTGCLWLSPETDLKRRDVPARELFFDAGVLIGARSQLIGDDLVDVQGG
                     LWSTAQRKRAHELLRKVRGDGLRAQLDLLVASKLLDAKDFPQRQADYVVELVCRALVP
                     QRGTFRLLGTELPPGEQTMLPLQPRLLLLHAIRRGMPLDVLTSIVGPLSTILVPTNLA
                     LPQVGGVVLQNLGLSQPEEDVLLSFDGDRSLQDIFSATGIGEHSLYGLSFCLLTLGAL
                     VHPHNLSQDDRKKLLVLRQARARAKAQSEAMAVVQKKARLSETADYFTLLGVPAGASI
                     EEVEAAYARERAAIAPAGLPQRSRATMERELRQIAMVLDEAYAVLADPVLRACYAPTA
                     KRLPE"
     gene            complement(2081..2794)
                     /locus_tag="PKL17_14470"
     CDS             complement(2081..2794)
                     /locus_tag="PKL17_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45989.1"
                     /translation="MAKQKGSAFELGLDEAGRGPMLGPLVMAAVALCADTAQALQALG
                     VTDSKHFGAGEKAHEKRRALVAEIVAVASFVEVVVVDVAEIDAAVRKGQLNHLERQVA
                     AGLIARAPHCETIYADGKRLFEPLQKQFPQLRAQDKADSAFVSVAAASLCAKVRRDDL
                     WFAICERYRPEFPSELTGLAGGGYVNPATRRFLRAFVSKYHRLPDETRRSWPRDFLAD
                     LLPDENPQVSGQPLLLPWG"
     gene            complement(2823..3716)
                     /locus_tag="PKL17_14475"
     CDS             complement(2823..3716)
                     /locus_tag="PKL17_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45990.1"
                     /translation="MILQLSALSSVAQSLALRIVVEGGCPEPAAVMSHLRLIDSSLID
                     RDATARLEPIDDKLRVELRSGDGALLVVQDLLLGPSCDTLAAAASVVIATALGSAKSG
                     ELPLYGPIPIPLPPLPAHVPMSPRPLSLELGLSVGLEPSWPRPGGGGFGLLQLAPTKA
                     GWNRLGAVVLVGGTSLKQQAFLQGSVDFTRLQLAVGPRLRLSVGSWLFDLFATVSPAL
                     TLVYGRDFPSTFASQGFDLGLGGGVRAMAKLGALLPFVMLHASGFVREQRLHVLGADA
                     TSALAPYSVQLGLGLSWLALP"
     gene            complement(3743..4063)
                     /locus_tag="PKL17_14480"
     CDS             complement(3743..4063)
                     /locus_tag="PKL17_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNK45991.1"
                     /translation="MMKQTMAERVESKLRAALTPTHLEVVNESHQHSVGEGAESHFKV
                     IIVSPQFEGKSLIEQQRLVYGVLSEELKAAIHALALKTVAPSKWQAQTTDYQSPACRG
                     GSKA"
     gene            4162..4941
                     /locus_tag="PKL17_14485"
     CDS             4162..4941
                     /locus_tag="PKL17_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNK45992.1"
                     /translation="MEESRYFELDAKLESLRIRKAAEDPRILAAYGDKVDIGWLYHDN
                     ALEGVVLTYGEIRDALDRKIISDVSLVSLYEEIRSHKAAIDFVRETAKDHAAQKKRAG
                     LITVELIKQLHEYLTPEDKAKGNPYRKDNPLHRSYFHEIAPADKIPMRMRKLCEWLDE
                     EETVSMHPIARAAGAHFRLMAIYPWTKNSGKVARLLMNLLLIRDGYPIAVIHSMERQR
                     YYDSLRAENGYLGQLVAESLTGYVNTTLRFFEELAELRRAG"
     gene            complement(4949..7558)
                     /locus_tag="PKL17_14490"
     CDS             complement(4949..7558)
                     /locus_tag="PKL17_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45993.1"
                     /translation="MTDQDVSTRGGGGRRWLYGLLTFFGVGLLGYRGYVAFQQEKRDE
                     AQRRHDRAVSEHFATVKAAQGRGDHTSALWWLDKAYLSGNDDARMRFLLPHLSQKLRQ
                     TVAILNHDGEVTSVSFVGGGQRALSASADGTARLWSLPDGKLLRKLDKIDGVLTAAWQ
                     SAGGERLVTLDQEGVARLYGDTGTQPLAQLSDPGGPMGPLTQISLSQAGDRVLAIVAA
                     EQQRGARLWNGADGKLVGRLDGHEAPIYSALLGPNGKRVATASPGTVKLWDAATGALV
                     ANLQGHTDQVRDIVFSPDGKRLVTSSSDRTARLWDGETGALVAVLEGHDGTVHSASFT
                     LANGRAVMTVSADGTARLWDAQTGASMFSPVSHPRRSQVLAMTKDGGRMVVLSENGVP
                     ELWERIVGHMRAQLLGHVGPVLATAFSPTGRRIVTTGADGTVRIWDGRSGQSLLTLRG
                     HHQRVSAVAFSPDGQYVLTGSRDGTARLFRSQIAELPMPLAGHLSEVVSLAFTPDGKR
                     LLSASRDGKAAVWDLKDAERLAELPSRGISEARWLPQRDGIQQILTIVPGGTSSTGRA
                     PALLRYFDGQSYAEKLSQGGDLALGQVLSVSPTGDQLVTLLDGKALAWSRQDGAKMPK
                     AVGALAAQNDEPLSVVFDPSGQHLATGGRRGEVVLYDAKQGFAPFVLTGHQGAVRTLQ
                     FSPSGKRLASGSADGSARLYDIDSHKLVATMSGHSGEVRTVQFSGDGSRLLVIDSGER
                     AWLRDGQDGKLVAALAKHSSKEYAVMAMSSDGTRLASCGDELRIWDAQHGELLAQLDD
                     LGRADVLSACAFSPDGALLATGSQQGQVQLRDVHLETRSSAVVHQDLLAIPAYSEADE
                     RLRKTMLQMIDRK"
     gene            complement(8068..8880)
                     /locus_tag="PKL17_14495"
     CDS             complement(8068..8880)
                     /locus_tag="PKL17_14495"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNK45994.1"
                     /translation="MSVLQRGDEDPSVHSLQELLLGLRAPLADPPAHFGLWTEASVFV
                     LQHCAGIEPDGVYGPQTAAILSSGHLPADFAKLIVNPLRVIAGVPYKSQRDNEHDPSS
                     TCNVTALAMAMDYLGVSKSDPQAQLEDELYELIRSPSGLAFYKQTNPQLHEKGIPANQ
                     VYDNLVWAAEQYGLRASFSGKRSWQQIAQEIQDGRPVLLSTTLTASGHIVLLVGLTAT
                     GDLVCHDPYGDFKRGYKSKAGAFRIYPSHCIAQHVKEVHSDEKWALFLRALA"
     gene            complement(8943..10247)
                     /gene="chrA"
                     /locus_tag="PKL17_14500"
     CDS             complement(8943..10247)
                     /gene="chrA"
                     /locus_tag="PKL17_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNK45995.1"
                     /translation="MQTAPGTHRLEPARVEQPTSLRELCALFVRLGATAFGGPAVHLS
                     LMHREVVERRKWIDEQRFLDLLSATNLIPGPNSTEMALHIGWQRRGFWGLVTAGLSFI
                     VPAMLITLGLAVIYVRYGRLPGVSSLLYGIKPVMLAVVLAAVASLAKKALPGTTEKVL
                     AGIGMVASLVGVHELMVLFGIGVLVVLAARLRSEKESPKTVRADSGKETEGESQRESE
                     KESQSDSQNDSQNDSQGSLLPIFPSVPLALSASAKLVSLSSVFALFFKIGGVLYGSGY
                     VLLAFLRADLVLRLGWLSESQLIDAIAVGQMTPGPVFTTATFVGYLLLGTWGALAATA
                     GIFLPAFGLVAVSGPLIPRLRSLPSLGRFLDGVNVGSWALMVAVTLQLGKAALSDLPA
                     VLVAVGSLLAILRFRTNSTFLLVAGALIGFLRHHWFEIGRFF"
     gene            complement(10257..11090)
                     /locus_tag="PKL17_14505"
     CDS             complement(10257..11090)
                     /locus_tag="PKL17_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45996.1"
                     /translation="MNGRTRSRVGFCGLWSLLLGIAGIAGGAGCGSDVATGDALSDDG
                     VELAAITPPASLIKLLSTKPYVEDRCVSATYPGWPYAAQRCTYTSGPLTTSVTVANPS
                     AARAAAWIVDSATEIPRLSALQGSQQAAYEEGLKAIGLAMLYQSSRIFPLVGGIIEDM
                     GTGYKNYPFEKGITQGCSTGCYCRINSLHRTEYCRYRAAKKIEGESACLARVGTSGLT
                     AAWGAECLNNHASAWTSDRNEHFRAKAYIANQTVASKCPTATSCTPSQVVAAVKTGFG
                     L"
     gene            11169..11885
                     /locus_tag="PKL17_14510"
     CDS             11169..11885
                     /locus_tag="PKL17_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNK45997.1"
                     /translation="MTETDGSVENRRHYRYYDLILGGFITVLLCSNLIGPAKSCMVAG
                     IKFGAGNIFFPISYIFGDVLAEVYGYARARKVIWAGFFALIFATVMAQVIIHLPTNPD
                     EPLNVSYQPAVELLFGGTWRIVAGSMVAFWVGDFANSYVLTKMKILTQGRHLWSRTIG
                     STIIGQALDSLIFYPIAFYGIWQNSELIKILAFNWLFKISIEVIFTPITYAVVGFLKR
                     EEQEDYYDHKTNFSPFSLRD"
     gene            complement(11854..12510)
                     /locus_tag="PKL17_14515"
     CDS             complement(11854..12510)
                     /locus_tag="PKL17_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNK45998.1"
                     /translation="MRILSNTAIALDGRINTTEGGYIQLGSAHDHRLMSKLRAEADAV
                     LVGGGTYRNGPHPLIPSAPDPSKTWWNVIVTRSLSLPLPSDFVLHPQVRPLVLSRRQP
                     IPASIPAKVEVELFDGEGDPTVDWMVSCLVRRGIQTLLIEAGGELLFQFAAADRLDEV
                     YVTLCPLLIGGHNTPSLMGGRGFSLTDMRRLSLLSSQVIGDEIYLHYQVNREVKTGKS
                     "
     gene            complement(12507..13097)
                     /locus_tag="PKL17_14520"
     CDS             complement(12507..13097)
                     /locus_tag="PKL17_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK45999.1"
                     /translation="MALKDGLHLEIEHKFVLDEQFDRAAFCAKVEVLGPIRTVHTEVD
                     ETYYLVKATPELIYRHRYDENHQDLTYKTFRQGDIEVRKEVRIALDHQRGNQKAQVTS
                     FLSPLHIHWQSDLKKTLWVYDFADCEAVYYVAKSADRTVICVEFEALSAPSVESALAT
                     LASYERQLGFSDQERSRRSLFELLFADQLNKQGDAA"
     gene            complement(13097..13579)
                     /locus_tag="PKL17_14525"
     CDS             complement(13097..13579)
                     /locus_tag="PKL17_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNK46000.1"
                     /translation="MRIAKDSDLALIVATYNATIASRRVTADLVPVTVDSRRPWFAAH
                     DPSVRPLWIVEEAGVYRGWLSFQSFYGRPAYAGVAEVSLYLDGAAQGRGLGAAVLSWA
                     IAEAPKYGLHTLLGFIFGHNEPSLKLFYRLGFVKWGHLPEVANMDGVMRDLLIVGRKV
                     "
BASE COUNT         2935 a         4487 c         3678 g         2498 t
ORIGIN      
        1 gcctgcgcga tctgggacgg gcagcccagt ttgtcgaaca gctcgcgctg cggcagaagc
       61 ttctgacgat ggggatggga ctgcggcaag agctgctcca agacacgctc agccggacgg
      121 tgcccgatgc gagccaagcg acttgggatg atgtgctcgg gacgatgctg gccaatcacc
      181 tgttgcaaag accggcggcg ctggtgccca tcatgcgtcg cgatctggtg ccgctgcgac
      241 ggatgattgg accgcaagac gagcccaagc aggcgcagct gcccgagctt ttgcgcgccc
      301 gctgtaccaa gcttatcgaa gagcgcatcg ccgatcgcca gaccgatcca gagagcgcgc
      361 gacttctgct tggaccggca cagcgcgcat ttgtcgaacg ggtacagagc cagcttgcgc
      421 agggactggc gacgctgcct gacgtactcg atgacgaagc cgccgatgtg gtgccgcgcg
      481 tgcccggact gctactcggg tagtcgtttt gccgtcggtg cgtaacaggc ccgcagcacc
      541 ggatctgcaa gcaccgcata cgcttcatcc agcaccatcg cgatctggcg cagctcgcgt
      601 tccatcgtcg cacgactgcg ctgcggcagc cccgccggag caatcgccgc acgttcccgc
      661 gcataagccg cctccacttc ctcgatcgaa gcgccagcag gcaccccgag cagcgtgaag
      721 tagtccgcgg tttccgacag ccgcgctttt ttctgcacca ccgccatcgc ttcgctctgc
      781 gctttggccc gtgcccgcgc ttgccgaagc acgagcagct tcttgcgatc gtcttgcgac
      841 aggttgtgtg gatgcacaag cgcgcccagg gtcaaaagac aaaacgacag accatagagc
      901 gagtgttcgc cgatccccgt cgctgaaaag atgtcttgca ggctgcgatc gccgtcgaat
      961 gaaagcagca cgtcttcttc tggctgcgac agccccaggt tttgcagcac cacgccaccc
     1021 acctgcggca gcgccaggtt cgtcggcacc aaaatggtcg atagcggccc cacgatgctt
     1081 gtcagcacat cgagcggcat tccccgtcgg atcgcatgca aaagcaagag ccgcggctga
     1141 agcggcagca tcgtctgttc ccccggcggc agctccgtcc cgagcagacg aaacgtaccg
     1201 cgctgcggca caagtgctcg acagaccagc tcgacgacgt aatcggcttg ccgctgggga
     1261 aagtctttgg catcgagcag cttgcttgcg accagcaaat cgagctgtgc acgcagaccg
     1321 tcgccgcgca ctttgcgcag cagttcgtgg gctcgcttgc gctgcgccgt cgaccacagc
     1381 ccgccttgca catccaccag atcgtccccg atgagctgcg agcgcgcccc gatgagcaca
     1441 cccgcgtcaa aaaacagctc acgcgctggg acatcgcgcc gcttcagatc ggtttcaggc
     1501 gaaagccaca gacagccggt gtagcccgtc gcgaccgccc agccacaaag ctgcgccgcc
     1561 gaaatgtcgg tctgtgaacc gcccggcgga gccaagcgca accaacgaaa cgtcggacgc
     1621 ggttcggtct cgtgtgggac aagttctttt tgcgcaagcg gaaccagtgc gtctgccgac
     1681 acgctggccg gcgcggggat ggttaccgga tcgcgctgca acgtccgcag ctctgcgtcc
     1741 cgacgaacca ctgcgcgtgg tggacgcgac gacggcgaaa gccagcgctt gtcatcgccc
     1801 ggttcggcca aatcatcaat tcgcagcagc cgcagcttgg ccagcgacct tagctgaagc
     1861 tgtgaacccg atcgcagcga cagaagcttt ccttcataca aaggcggcgg tggtggcctt
     1921 gccccaagag gaatccgcgt cggcagcggc gtgttgtcct cttcaatcgg cacaggcaca
     1981 gccccggtca caaacggaac ggtgtcgtcg ccatcggatt tgcgggtggt cacgacgcca
     2041 gtctaggtcg atttgggcac gaccggaagc tttggccaaa ctatccccac ggcaagagga
     2101 gcggctgccc agaaacctgc gggttttcat cgggcaaaag atccgccaaa aagtcgcgcg
     2161 gccagcttcg gcgcgtctcg tcaggcagcc tgtggtattt gctgacaaag gcgcgcaaaa
     2221 agcggcgcgt cgccggattg acgtaaccgc ccccggccaa gccagtcagc tcgctcggaa
     2281 actccggtcg atagcgctcg cagatcgcaa accacagatc gtcccgacgg accttagcgc
     2341 acagcgaagc cgcggcgacc gacacaaacg ccgaatcggc tttgtcctgc gcacgcagct
     2401 gcggaaattg cttttgcagc ggttcaaaca ggcgctttcc gtcggcatag atcgtctcgc
     2461 agtgcggcgc ccgcgcaatc agtcccgccg ccacctgccg ttccaagtgg ttgagctggc
     2521 ctttgcgcac cgcagcatca atttcggcca catcgacgac caccacttcg acaaacgacg
     2581 ccacagcgac aatttcggcc accaaggcac gccgtttttc atgggctttt tcgcccgcgc
     2641 caaagtgttt gctgtcggtg acgccgagtg cctgcagcgc ctgcgcggta tcggcacaaa
     2701 gcgccaccgc cgccatcacc aaaggtccca gcatcggccc acgccccgct tcgtcgagac
     2761 ctagctcgaa agcactgccc ttttgctttg ccatctgcgg cttttagcag gaaatacgac
     2821 gactacggaa gcgccagcca cgaaagacca agacccagct gcaccgaata aggagccagc
     2881 gccgacgtcg catctgcccc gagcacatgc agccgctgct cgcggacaaa gccgctcgcg
     2941 tgcagcatca caaacggcag aagtgcgccc agcttggcca tcgctcgcac gccgccgccc
     3001 agccccagat caaagccttg gctggcaaat gtcgacggaa aatcccgccc gtacaccagg
     3061 gtcagggccg gcgaaaccgt cgcaaacaga tcaaacagcc acgacccgac cgaaagccgc
     3121 agccgcggtc caacggcaag ctgaagccgg gtaaagtcga cactcccttg caaaaatgcc
     3181 tgctgtttga gcgacgttcc cccgacgagc accaccgccc ccaggcgatt ccagcccgct
     3241 ttggtcggcg ccagctgcag aagcccaaaa cccccgccgc ccggtcgcgg ccacgaaggt
     3301 tcaagcccga ccgaaagccc cagctccagc gacagcggac gcggcgacat cggaacgtgc
     3361 gcaggcagtg gcggcagcgg tatcggaatc ggtccgtaca gcggcagctc gccacttttt
     3421 gccgaaccaa gcgccgtcgc gatcaccacc gaagccgccg ccgccagcgt gtcacacgat
     3481 ggcccaagca gcagatcctg caccaccagc agagcgccat ccccgctgcg cagctcgacc
     3541 cgcagcttgt cgtcgatggg ctccagtcgc gccgtggcat ctcgatcgat aagcgagctg
     3601 tcgataagcc gcagatgcga catgaccgca gccggctcgg gacagccccc ttcgaccaca
     3661 atccgcagcg ccaaagactg cgcaaccgac gacagcgcgc tcaattgcaa gatcaccctt
     3721 ttagacgatc accgatcgac gactacgcct tgctgccccc acgacacgcg ggcgattgat
     3781 agtcggtggt ctgtgcctgc catttcgacg gcgccaccgt tttcaaagcc agcgcgtgga
     3841 tcgccgcttt gagttcttcg gacagcacgc catagaccaa gcgctgctgc tcgatcaggc
     3901 tctttccctc gaactgcggc gagacaatga tcactttgaa gtgactctct gcgccttcgc
     3961 ccacgctgtg ctgatgactt tcattgacga cttcaaggtg tgtaggagtg agtgcagcac
     4021 gcagcttcga ctccacgcgc tccgccatag tttgtttcat catgcgaacc tttttaccgt
     4081 aaagcccacg taggtgcgca caacttcgta tttttatggc acactcgcga gccgtacgac
     4141 tgtccttgag gagcctgctt catggaggaa tcccgctact ttgagctaga tgccaaactg
     4201 gagtccctac gtatccgcaa ggcagccgaa gacccgagaa tcctggcggc atacggtgac
     4261 aaggttgaca tcggatggct ctaccacgac aacgcactag aaggcgttgt cctgacttat
     4321 ggagagattc gcgatgcact cgatcgcaag atcatctcgg acgtaagtct ggtgtcgctt
     4381 tacgaagaga ttcgcagtca caaagccgcg attgactttg tgcgtgaaac tgcgaaagac
     4441 catgccgcac agaaaaagcg cgctggtttg attacagtgg agcttatcaa acagcttcac
     4501 gaatacctga cgccggaaga caaagcgaaa ggcaatccgt atcggaagga caatccgctg
     4561 caccgctcgt attttcacga gattgcaccg gcggacaaaa tcccgatgcg gatgcgcaag
     4621 ctgtgtgagt ggctcgacga agaagaaacg gtgagcatgc atccgattgc gcgtgctgcc
     4681 ggagcgcact tccgtctgat ggcgatctat ccgtggacca aaaacagcgg caaagtggct
     4741 cggcttttga tgaacctttt gctgattcgc gacggttatc cgatcgcggt gatccactcg
     4801 atggaacggc agcgctacta cgacagcctg cgcgccgaaa acggctacct gggccagctc
     4861 gtcgctgaat cgctgaccgg ctacgtcaac accaccctcc gcttcttcga agaactcgcc
     4921 gagctgcgcc gcgccggtta gtccgccgct acttgcggtc gatcatttgg agcatcgtct
     4981 tgcgcagtcg ctcgtccgcc tcgctgtagg ccggaatcgc cagcaagtct tgatgcacca
     5041 ccgccgatga tcgcgtctct aggtgaacat cgcgaagctg cacctgaccc tgctggcttc
     5101 ccgtcgccag aagcgctccg tcgggactaa acgcacaggc tgacagcaca tcggcccgtc
     5161 ccagatcgtc cagctgagcc agcagctcac cgtgctgggc atcccaaatt cgcagctcat
     5221 cgccacacga agccagccgc gtgccgtcgg agctcatcgc catgacggca tattctttgc
     5281 tcgaatgctt ggccagggct gccaccagct tgccgtcttg tccgtcgcgc agccaggctc
     5341 gttcacccga atcgatcacc aaaagacgcg atccatcacc ggaaaactgc accgtgcgga
     5401 cctcaccgct gtgaccactc atcgtcgcga ccagcttgtg cgaatcgatg tcgtaaagtc
     5461 gtgccgatcc gtccgccgat ccgctcgcca gtcgctttcc cgaaggagaa aactgaagcg
     5521 tgcgcaccgc cccttgatgt cccgtcagaa caaacggtgc aaagccttgt tttgcatcgt
     5581 acaagaccac ttcgccgcgc cgtccacccg tcgccaaatg ctgtccgctc ggatcgaata
     5641 ccaccgacag cggctcgtcg ttttgcgccg ccagtgcccc gaccgctttg ggcatcttgg
     5701 caccgtcttg ccggctccac gccagcgctt tgccgtcgag aagtgtcacc agctgatctc
     5761 ccgtcggcga caccgaaagc acctgcccca gcgccagatc gccgccttgg ctcagctttt
     5821 cggcatagct ctgcccatca aaatagcgaa gcagggccgg cgctcgtccg gtgctgctgg
     5881 tgccacccgg cacgatcgtc agaatctgct gaatcccatc gcgttgtggc agccagcgcg
     5941 cctcggaaat gccacgactt ggcagctccg ccagccgttc ggcgtctttg agatcccaga
     6001 ccgccgcctt gccgtcacga ctcgccgaca acagacgttt tccgtcgggc gtaaaagcca
     6061 gcgataccac ttcgctcaga tgccccgcga gcggcatcgg cagctcggcg atctggctgc
     6121 gaaacagccg cgccgttccg tcgcgacttc cggtcagcac atactgacca tcgggcgaaa
     6181 acgccaccgc cgacacccgc tgatgatggc cacgcagcgt cagaagcgac tgacccgagc
     6241 gcccgtccca gatgcgaaca gtcccatcgg cccccgtcgt cacaatccgc cgccccgtcg
     6301 gtgaaaatgc ggtcgccaag accgggccga cgtggcccaa aagctgcgcc cgcatgtgac
     6361 cgacgatgcg ttcccacagc tcggggacgc cgttttcgct cagcaccacc atccgcccgc
     6421 cgtccttggt catcgccaag acttggcttc ggcgcggatg actgaccggc gaaaacatcg
     6481 acgcgcccgt ctgcgcatcc cacagccgcg cggttccgtc ggcgctgacc gtcatcaccg
     6541 ctcgaccatt tgccagcgta aacgacgccg agtgcaccgt cccatcatgt ccttcgagca
     6601 cggcgaccag cgccccggtt tcgccatccc acagccgcgc cgtccgatcc gagctgctcg
     6661 tcaccagccg tttcccgtcg ggcgaaaaca cgatgtcgcg cacctgatcg gtgtgacctt
     6721 gcagatttgc aaccagcgcc cccgtcgctg catcccacag cttgacggtc cctggcgatg
     6781 ccgtcgcgac ccgcttgcca tttggaccaa gcagcgccga ataaatcggg gcttcgtgtc
     6841 catcgagccg cccgaccagc ttgccgtccg ccccattcca cagtcgggcc ccgcgctgct
     6901 gctccgccgc gacgatcgcc agcacccgat cgccagcttg ggacagcgaa atttgtgtca
     6961 gcggccccat cggtccaccc ggatcggaaa gctgcgccag cggctgcgtc ccggtgtcgc
     7021 cgtacagcct ggccacgcct tcttgatcca gcgtgaccag ccgctctcca cctgcggact
     7081 gccacgccgc cgtcagaacg ccatctatct tgtccagctt ccgcagcagt tttccgtctg
     7141 gaagtgacca cagccgagcc gttccatccg cactcgccga aagcgctcgc tgcccccctc
     7201 cgacgaaact gaccgacgtc acttccccgt catgattcag gatcgcgacg gtctgccgca
     7261 gcttctgcga tagatgcggc agcaaaaaac gcatccgcgc atcgtcattc cccgacagat
     7321 acgctttgtc gagccaccac agcgctgacg tgtgatcgcc tcgcccttgc gccgctttca
     7381 ccgtcgcaaa atgttccgaa accgcacgat cgtgccgacg ctgggcttca tcgcgctttt
     7441 cctgctgaaa cgcgacatag ccccggtagc caagcaggcc cacgccaaaa aacgtcagca
     7501 gcccgtacag ccaccgccgc ccgccaccgc cccgcgtcga aacatcttgg tccgtcatgt
     7561 agcgccactt tatcagacgc cgcccgagcc tgtacggcat caccaggaag gtcggtgacc
     7621 atccatcgac aaaaccaccg aagcgattgc cgaatcgggg caccaacaaa tacagccgat
     7681 cgcgatctta tccaccgcat acataaagcg ataggtaact acacatgacg acagaagagc
     7741 tttctaaaca acaatcctgg ggcccggttt tttcatctct tctaacgtct gcatttgtcg
     7801 taggcctgat cggattcgct gattgcgaaa gcgctccagt cacctaccgt ccaccactga
     7861 acagcagaac gacagatacc tacacaaacg atgttcagtg tgatgtagat accgttttgc
     7921 gatcaaactg catgtcgtgc cacgactcgc cgccgcggat gctaggaacg tccagcgcac
     7981 actgcaacgg ctgtcacacc gtcggtggaa caagcggagc gccaggccga atcatggccc
     8041 cctaaaccac cgcgttccgc agattcctta cgcaagcgcg cgcaaaaaga gcgcccattt
     8101 ttcatcggag tgcacttcct ttacgtgctg cgcgatgcag tgcgaaggat agatccggaa
     8161 ggcacccgct ttgcttttgt agccacgctt aaagtcgccg taggggtcgt gacagaccaa
     8221 atctcccgtc gctgtgagac cgaccaaaag aacaatgtgg ccgctcgcgg tcagcgtggt
     8281 cgacaaaaga actggacgtc catcctgaat ttcctgcgcg atctgctgcc agctcctctt
     8341 gccggaaaac gacgcacgca gaccgtactg ttccgcggcc cacaccaagt tgtcgtagac
     8401 ctgattggca ggaattccct tttcgtgaag ctgcgggttt gtctgcttgt aaaacgcgag
     8461 tccggatggc gagcgaatca gctcgtacag ttcgtcttct agctgggcct gcgggtcgct
     8521 tttgctgacg cccaaatagt ccatcgccat cgccagcgcg gtcacgttgc aggtcgagct
     8581 gggatcgtgt tcgttgtcgc gctgggactt gtacggaacc ccggcaatca cccgcagcgg
     8641 attcacaatc agcttggcaa aatccgcagg caagtggccc gatgaaagga tcgccgcagt
     8701 ctgcggaccg tagactccgt cgggttcgat tccggcgcag tgctgcagca caaacaccga
     8761 cgcttccgtc cacagaccaa agtgcgcagg cggatcggca agcggcgccc gcagacccag
     8821 cagcagttcc tgaagggaat ggacggaagg atcttcgtcg ccccgctgta gaacgctcat
     8881 acgcgaacaa tatcacgacc gaaacgcgca cgggcgggag cccacaagcg ctgcattttc
     8941 cgtcagaaaa accgaccgat ttcaaaccag tgatggcgca gaaaaccgat cagcgccccg
     9001 gcaacaagca ggaaggtgga attggtccga aagcgcaaaa tcgcaagcaa cgaccccact
     9061 gccaccagca ccgctggcag atcagacagc gccgctttgc caagctgcaa agtcaccgcc
     9121 accatcagcg cccaggaccc gacgttcacc ccatccagaa agcggcccag tgacggcaga
     9181 ctgcgcagcc gcggaatcag cggaccactt acggccacca agccaaacgc cggcaaaaaa
     9241 atcccagccg tcgctgcaag tgcgccccag gttcccaaaa gcaggtagcc gacaaatgtc
     9301 gcggtggtaa agaccggccc cggtgtcatc tgtccgactg caatcgcatc aatgagctgt
     9361 gattccgaaa gccatcccag gcgcaaaacc agatcggcgc gcagaaacgc cagcagcaca
     9421 tagccactgc catacagcac gccaccgatc ttgaaaaaca gcgcaaacac cgacgacaac
     9481 gaaaccagct tggcggatgc agacagcgcc agcggcacag atggaaagat cggcaacaga
     9541 gagccttggg aatcgttttg ggaatcgttt tgggaatcgc tttgggactc cttttcggac
     9601 tcccgttggg attctccttc ggtctccttt ccggaatcgg cacggaccgt ctttggcgat
     9661 tccttttcgc tgcgcagtcg tgccgccaaa acaaccagca cgccgatacc aaacagcacc
     9721 atcagctcgt gaacaccgac gagcgatgcc accattccga ttcctgccag cactttttcc
     9781 gtcgtgcctg gaagcgcctt tttcgcaagg gatgcgaccg cggccaagac caccgccagc
     9841 atcaccggct tgattccgta cagcagagac gaaacgccag gcaaacgtcc gtaccgaaca
     9901 tagatcaccg ccagcccgag cgtgatcagc atggccggaa caatgaacga cagacccgcg
     9961 gtcacaagcc cccaaaaacc ccgacgctgc cagccgatgt gcagcgccat ttccgtcgaa
    10021 tttggccccg gaatcaggtt cgtcgcgctc agcagatcga gaaaccgctg ctcatcgatc
    10081 cacttgcggc gctcgacgac ttcccgatgc atcagcgaca ggtgaaccgc cggcccacca
    10141 aacgccgtcg cgcccagccg gacaaacagc gcgcaaagct cacgcagcga tgtcggctgt
    10201 tccacgcggg cgggttccaa gcgatgggtc ccaggtgccg tctgcaaaga caggaattac
    10261 agaccaaacc cggtcttgac cgcagcgacc acttggcttg gcgtgcagct tgttgccgtc
    10321 ggacacttgc tcgccacagt ctggttggca atgtacgctt tggcacggaa atgctcgttg
    10381 cgatcgctcg tccaggcaga agcgtggttg ttcagacact cggcccccca cgccgccgtc
    10441 agaccgcttg tgcccacgcg agccagacac gccgactcac cctcgatctt tttggcggcg
    10501 cgataccggc agtattcggt gcgatgcagc gagttgatcc ggcagtaaca ccccgtcgag
    10561 cagccttgcg taatgccctt ttcaaacggg tagtttttgt agcccgtgcc catgtcttcg
    10621 atgattccgc cgacaagcgg aaagatccgc gacgactgat acagcatcgc cagtccgatc
    10681 gctttgagcc cttcctcata agcggcttgc tgactgcctt gtagcgccga aagccgcggt
    10741 atttccgtcg ccgaatcgac aatccacgct gcggcgcggg ccgccgaagg attcgccacc
    10801 gtcacggaag tcgtcagcgg tccacttgtg tacgtgcagc gctgcgccgc gtaaggccaa
    10861 cccggatagg tcgcggaaac gcagcggtct tcgacatacg gcttggtcga cagaagcttg
    10921 atcagcgatg ccgggggcgt aatcgcggcc agctcaacgc catcgtccga cagcgcatcg
    10981 cccgtcgcga catccgatcc acaaccggca ccgccagcga tcccagcaat ccccaaaagc
    11041 agcgaccaca gaccgcaaaa tccgacgcga gagcgagtgc gaccgttcat gaggaacctc
    11101 caccccggct tgtatcacag actgacttcg gccttctctt tgtgaagatg agccggtata
    11161 aggggtacat gaccgaaacg gacggctccg tcgagaaccg ccgccactat cgctattacg
    11221 acctcatcct tggcggattc atcaccgtcc ttttgtgcag caatctgatt ggcccggcca
    11281 aaagctgcat ggtcgcaggc atcaagtttg gcgccggtaa cattttcttc ccgataagct
    11341 acatttttgg cgacgttctc gccgaggtgt acggctacgc acgcgcccgc aaggtcatct
    11401 gggccggctt ttttgccctg attttcgcga cggtgatggc ccaggtcatc atccatctgc
    11461 ccaccaatcc cgatgaaccg ctcaatgtca gctaccagcc tgccgtcgag ctattgttcg
    11521 gcggaacctg gcggatcgtc gcaggttcaa tggttgcatt ttgggtcggc gattttgcca
    11581 attcctatgt gcttaccaag atgaaaatcc tgacccaagg acgccatctg tggagccgca
    11641 ccatcggatc aacgatcatc gggcaagcac tggatagctt gatcttctat ccaatcgcct
    11701 tttacgggat ctggcaaaac agtgagctga tcaaaatatt ggcgttcaac tggttattta
    11761 agatatctat cgaagtcatc ttcacgccaa ttacctacgc agttgttggc ttcctcaagc
    11821 gagaggaaca agaagattac tacgaccaca agactaactt ttccccgttt tcacttcgcg
    11881 attgacctgg taatgcaaat agatttcatc gccgatcact tgcgatgaca gcaggctcag
    11941 gcgccgcata tcggtcaatg aaaagcctcg tccgcccatc agcgacgggg tgttgtgtcc
    12001 gccgatcaac agcgggcaca atgtcacata gacttcgtcc agtcgatccg ccgctgcaaa
    12061 ctggaacaac agctcgccgc ccgcttcgat aagcagcgtc tgtatcccgc gccgcacaag
    12121 acaagacacc atccagtcca ccgtcggatc gccttctccg tcgaacagct cgacctcgac
    12181 tttcgccgga atggaagcag gaatcggttg ccgacgcgat aggacaagcg gtcgcacctg
    12241 gggatgaaga acaaaatcgg acggcagcgg caacgaaagc gagcgggtga cgatcacgtt
    12301 ccaccacgtt ttcgacgggt ccggcgcgct cggaatcagt ggatgaggtc cgttgcgata
    12361 cgttccaccg ccgaccagca ccgcgtcggc ttcggcgcgc agcttgctca tcagccgatg
    12421 atcgtgcgct gagccaagct ggatatagcc gccctcggtt gtgttgatac ggccatccag
    12481 cgcaatggcg gtattgctca gaatcctcat gcagcatccc cctgtttatt caactgatca
    12541 gcaaataaca attcaaacaa ggaacgacga ctgcgctctt ggtccgaaaa tcccagctga
    12601 cgctcgtagc tggcaagcgt cgcaagcgcc gactccaccg acggggccga aagcgcttca
    12661 aactcgacgc aaatgacagt gcggtcggcg cttttggcga cataatacac ggcctcgcag
    12721 tcagcaaaat catacaccca caacgtcttt ttcagatcgc tttgccaatg aatgtggagc
    12781 ggtgacaaaa aagatgtcac ttgcgctttt tggttgccgc gttggtggtc aagtgcgatg
    12841 cggacttctt ttcttacctc gatatcaccc tgacgaaatg ttttatatgt caaatcttga
    12901 tgattttcat catagcgatg ccgataaatc agctcaggtg ttgcttttac caaataatag
    12961 gtttcatcaa cttcggtatg gactgtccgg atcggcccaa gtacttcgac ttttgcacaa
    13021 aaggctgctc gatcaaactg ttcatcaagc acgaacttgt gctcaatttc cagatgaaga
    13081 ccgtctttca gcgccattac acttttctcc cgacgattaa cagatcccgc atgacaccat
    13141 ccatgtttgc cacttccggc aagtgacccc atttcacaaa gcccaagcga taaaacagct
    13201 ttaagctcgg ttcattgtgg ccaaaaataa agccgagcag cgtgtgcagc ccgtatttcg
    13261 gtgcctcggc aatcgcccaa gacagcaccg cggccccaag tcctcgtccc tgcgccgccc
    13321 catccagata gagcgacact tcggcaaccc cagcgtaggc cggtcgacca tagaagcttt
    13381 gaaagctgag ccagccacga taaacgccgg cttcttcgac aatccacagg ggccgcacgc
    13441 ttggatcgtg ggccgcaaac cagggccggc ggctgtcgac agtcaccgga accagatccg
    13501 cagtgacgcg acgcgacgca atggtcgcat tgtacgtcgc cacgatgaga gcaagatcgg
    13561 aatccttggc aatgcgcagc gtcggaaaag gttgttct
//
LOCUS       DAORGN010000137        13594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1874_length_13594_cov_5.553586, whole genome shotgun sequence.
ACCESSION   DAORGN010000137 DAORGN010000000
VERSION     DAORGN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13594
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_13594_cov_5.553586"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKL17_14530"
     CDS             <1..471
                     /locus_tag="PKL17_14530"
                     /inference="COORDINATES: protein motif:HMM:NF025585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HNK46001.1"
                     /translation="NSLDIVCAFTQASALTSQIFECWGGDDEYVKGFASSTTTKGSVQ
                     SPTGKIKAWAGIVNDPAFFNKADLTTAISTIKTSLGNNSWTRDAAQCATTGSAWGTAQ
                     ALAMRNGLSATKVDAYNKQNVLAIVLSVDPQYLTQRGNSPLLGVWASTKKRIPK"
     gene            480..1184
                     /locus_tag="PKL17_14535"
     CDS             480..1184
                     /locus_tag="PKL17_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46002.1"
                     /translation="MTKKLFASVLLLAGLTSVGCDKTGTNFENVPYEFFDLGTGDGGS
                     SLIPGKPLADAVQYDRVGRVLVSTMMVNPFGLFKDTAYTDEYKDRYNGAVPLNGWLPF
                     SAQPYIQASLGIWDSLDVNCGNQIGANTQVSATRYQKLGDLLADDRLWVDTTKTSCKV
                     YMGVEQKFINPATPDDCGGRPLVEIPSSGVAKPFDAVDQTYNLLIGAGATVTDGVASD
                     SDGNPGGLFPFLVDAK"
     gene            complement(1220..3676)
                     /locus_tag="PKL17_14540"
     CDS             complement(1220..3676)
                     /locus_tag="PKL17_14540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNK46003.1"
                     /translation="MAGSSTRKLILAEKPQVARDLAKVLGVTGRSDGALTSDRYVITW
                     CIGHLVELCEPHEYDPAWKPWRLDKLPILPQPFKLRPIRSGFKQWKVVRALLKDRSFS
                     SVVNACDAGREGELIFRLCYELAGCRLPIERLWISSLTEQAIGHGMTHLQPGQAFDDL
                     ARAARCRSEADWLVGMNATRAVTVWRRGQQSVLCSLGRVQTPTLGILVLREQQIRRFV
                     PSDYFEVQAQLRRKNDPVQFVAQWQFGKLRRLASQTIADELAARDRPAPVVVDSVEEK
                     RLREPPPLLFDLTSLQRTCNRRFGWSAQHTLSVAQKLYEEHKLLSYPRTDSRYLGRDL
                     IGQLPRLFAAIAESQTYAGFVAQLERVSPPSRVFQDQRVTDHHAIIPTTTNLTPQRIG
                     SLPPDERKLHDVVVRRFVGAFFPDAEFAQTTVTVRVDAMGDAPKDKLEPDEHGRLCAL
                     PLPPDRYVAQGRQRLVAGWQDVAGLDGEGPSRRKSGGDDLGSDDEGDDSMVQQALPRL
                     GKGDVLVGAFVVQKKQTKAPPRFSEAALLSAMESAGKQLSDEALRRAMKDSGLGTPAT
                     RAAVIETLISRQYIERRGKQLWPTPLGIDLIEKLPDPSLASAELTGQWEARLNRMARG
                     EDRATDFMSGIASYVEQFIGKVRGLPPPAAVALPTDAPVRAPAKSHRRRSRAASSSAT
                     RSSSEKAVRRDTVRKSRLDSVAKPRRSSAASSRPSPGSPLVPTVCCPRCQSASMIWGK
                     QAWGCGDYRNCRLVVPFAIDGVSLSLANLGKLLSTGKVTLSRQGKRLSLRLKPGQDPA
                     VAVETKKSAPSVDRQTLEPS"
     gene            3898..4161
                     /gene="rpsT"
                     /locus_tag="PKL17_14545"
                     /pseudo
     CDS             3898..4161
                     /gene="rpsT"
                     /locus_tag="PKL17_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
     gene            complement(4460..5602)
                     /locus_tag="PKL17_14550"
     CDS             complement(4460..5602)
                     /locus_tag="PKL17_14550"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNK46004.1"
                     /translation="MATEAKVLLVGCGGIGGIAAAQLSRAGCDVTVVTGNPEIERVIS
                     EQGIRVRDIDGKEWRARPAQAVRSARELSGRYDVCLVATKLTTLGDVLGEVTPVLHEN
                     APILCLQNGLPETHAAKVVGDDRVVGCVVGFGATLIEPGFSARTSSGGFQIGRLKPRS
                     DDAELARLATLLGRGVPTKVVHDLAGVRWSKLAINCATSTLGAVGGDTLGRLLSHRFV
                     RRLVLEVWRELCAVADCEGIRLAKVAGTIDIAKLALSDEDKRHSVGSLKLFFKHSLLL
                     GIGLKFRRMRSSMAVAIERGRKPEIDYLNGEVVRRAAQHGIQAPLNRELVSMVHELVA
                     KRAKPGLDTLYGVYLRLENGQLGTTGQGPSMMPRRGEPSTTTLRIE"
     gene            5783..6472
                     /locus_tag="PKL17_14555"
     CDS             5783..6472
                     /locus_tag="PKL17_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNK46005.1"
                     /translation="MHEKPLFSTSAAFYDVDGTLIKTNIVHSFAYYALNQPTPLRSAL
                     KGLQTAASIPFFVVADKVSRKLFNEFFYRYYAGHSEDRLIILAEDLFDEVIRPSIFPG
                     VPDLIEESRRAGVRQVLVSGGLDFTIWPLARYLGIPKTDVIANRLEFSKGYATGQLCP
                     PFVGGATKAHIMRDFAGKNGIDLGKSWAYTDSYSDYPMLAAVGRPTVINPDLRLKLVA
                     RSYDWPIIDLR"
     gene            complement(6479..7357)
                     /locus_tag="PKL17_14560"
     CDS             complement(6479..7357)
                     /locus_tag="PKL17_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46006.1"
                     /translation="MENDSPFVFAATASSFEASVLERSHEVPVLVDFWAPWCGPCRVL
                     TPILEKCVAAQAGKVLLAKVNTDEQQDLAARFRISGIPAVKAFHKGQVVSEFVGARDG
                     RFVDSFISRLLPKPGEREVSEAASQLAMGVPQEAAQSLRQLLDGGLDGSVKSRAQVLL
                     AESLLLSGHASLAEVGELLRAVDPRSVESDRGEELQSVVDFLLAADEDGGFALATERL
                     TQNERDSAARFIVAAAQARQADFAAALENLLAIVARDRKFRDDGARKAMAALFQLLGP
                     NSELSHDFRRRLQVVV"
     gene            complement(7397..8248)
                     /locus_tag="PKL17_14565"
     CDS             complement(7397..8248)
                     /locus_tag="PKL17_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNK46007.1"
                     /translation="MTVIRGAARLVVDSSDSLLSQLPDGVAAELRGPLLRMDRLCALS
                     VVAVARLLSAHPPLRRDVNGDDVAIVIGTAHGCHKTDEEYYLSYLQGQPSPRLFAYTL
                     PSSPIGELSIFYRLRGPGLALCVGRASGLVALAEAETLLQTGQAAACVVLAVEVSGPA
                     LSPVQLSDAAVALWLTADRDGIDPALPRLVSAAESFIPDDPTTALARALASQPGGEVC
                     CDGETARLAGAALDDRAVVADVPDGSVAGLWRLVDVLSETGSGLLQPCSVAVVDPSGQ
                     AAVVTLN"
     gene            complement(8245..9465)
                     /locus_tag="PKL17_14570"
     CDS             complement(8245..9465)
                     /locus_tag="PKL17_14570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNK46008.1"
                     /translation="MAQRVAITGLGSLCGLGENPDALFAGLLAGQTAVRHLPALVGTT
                     LSVGPLVSASLPDEDNSDGGAILSGQPRAGLFLLHSAQFALRQAGLGGPGAGDGRRIA
                     VCVATTLGEKSPWLVGIESKHKQVEPPSLAYGCGLLAEQLATRLGAERVRAVSTACCS
                     SNTALSLSLSWLRRGLCDVVVCGGVDVLSPFVVSGFRSLRAQSADVCRPFDRRRSGVN
                     LGEASACVILETESHARRRGQPILAFLAGAGLSQDAHHMTAPDPQGRGALLAMQRALF
                     DAELSTDAVDFVSSHGTGTPFNDQMEGRAIQQLLGDRCLAVPVNSIKGSVGHTLGAAG
                     LLEAVMAVQVLSSQKIPQTVGLEEVDPAFSLDLVQGKPRESAVRCVLSTASGFGGENA
                     AVVIASASDAGSLP"
     gene            complement(9465..10445)
                     /locus_tag="PKL17_14575"
     CDS             complement(9465..10445)
                     /locus_tag="PKL17_14575"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK46009.1"
                     /translation="MSNDSSKSALSLQHRYEGPFYRRIMLGGIKHIPTWLQRLTMPMW
                     GGIFYFLIRKAREAVLRNLETVAGKVGFWQTHRRGLVTFRNYAQMLTDSYKVHLGQPL
                     TMEVESIGRTPELVSLIREKGGIMATGHIGMWQVGPFLAEWRDLPPFYVARAEEPNPL
                     VQKWEQRFRDRFKVIYTTGSPFSALSIAKVLREKSVVGMQIDRVLSDTGLTVKMCGKD
                     ARFPMGPALLARVTGAPLIPSFFVIDESLGRPRLIHHVGPAVIVENTRDREADVRRAT
                     EAMVAIYEKIVCKYPTQWYQFFDFFDVRVPLPVDGIAPSASLPPTVSAEN"
     gene            complement(10450..11349)
                     /locus_tag="PKL17_14580"
     CDS             complement(10450..11349)
                     /locus_tag="PKL17_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46010.1"
                     /translation="MSFSHALLRTIKRNTVPLLLSLVVALSGCPQTDDSPPVVDSALP
                     PLVEDLGITVKTVGVFEDCSPDGVEPIDVRCASGLHCGIVLVGETGAEGYLTQCVPDD
                     AAALKENQECALAKVASPATNPARRYDRCGSGLGCVATADGTNRCKRLCALRTRGRCG
                     KSDLCVLPSPVTGVGFCNAPDNCEPVFPQTGCGKAKDGTPLSCYVLGDDKGTGTVCWS
                     RQRYGDSSGDLDAPCERSWNCQSGFACTTRSGRDSACRPYCTLPTPPDGGSPPDGGTE
                     VMCNAGLGTCHPLSGFERVGRCY"
     gene            complement(11355..13232)
                     /locus_tag="PKL17_14585"
     CDS             complement(11355..13232)
                     /locus_tag="PKL17_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46011.1"
                     /translation="MSFPTAVSPSLRRAGGLLSLTALLAASDCTPVRESIDTDSGNSM
                     MDPSDEEKKPGTITISPGNASITINGSAQMVNFRALSSKLGDVTNLATWTVSDSSVGS
                     IVKGKLTIQPTLQKGGSYKVFASLRGSTASAPLLIKLIAPDEVDSTAPPDAKDYFSGS
                     SGGPAPSIAYPFDKTMMAANVLQLAMQWQAAAGQKVFRVQVIGATYERSFYVGSSLCP
                     SSQCTYRIDDKLWSAVGHSAQGQDVQIVVSGSSGPGAVYGTSMPATVSFAPEDIKGGL
                     YYFSPTIRGIKRVPIGASKPVDFITNGDETGCAGCHAVSRDGKKVAVEFGSGQTRTGS
                     TVVDGIMPKMRAFPLSSSIAWNFAWFNPGADKLITNWSGTLKVRDAGSGAVLETVQPA
                     QIGGMGLGAMPEWSPDGKWLAFVRYTRASSYDFELVDSGDIVVMPYNSGSFGQATTVI
                     PAKVGSEVHFWPSWSPDSKWLVFNSQTCSGSCQQYNAVQTRLRLVKAIDDAGQPVDNG
                     TPIELLAGTHAPNNSNNWPKFAPFLQNGRISFIVYSARYKYGFSGGSQPQLFMFGLDL
                     DKARTGAQDPSYQPIWLPFQEPSTGNHSAIWTTDVVCATDGECPSEFRCVANMCVPRI
                     G"
     gene            complement(13385..>13594)
                     /locus_tag="PKL17_14590"
     CDS             complement(13385..>13594)
                     /locus_tag="PKL17_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46012.1"
                     /translation="AKAEKAPAKAEKAPAKAEKAPAAAEPAKKPKPAAADGKAADKSD
                     KKAEPKASAKKAPAEAAPAKPARRK"
BASE COUNT         2838 a         4536 c         3828 g         2392 t
ORIGIN      
        1 aactcgctcg acatcgtctg cgcgttcacg caggccagtg cgctgacctc acaaatcttc
       61 gaatgctggg gcggcgacga cgaatacgtc aaaggcttcg catcgagcac gaccaccaag
      121 ggcagcgtgc aaagcccaac gggaaaaatc aaagcctggg ccggcatcgt caacgatccg
      181 gcgtttttta acaaagcgga cctcaccacc gccataagca ccatcaagac cagcctcggc
      241 aacaacagct ggacccgcga cgccgcccag tgcgcgacga cgggcagcgc atggggaaca
      301 gcccaggcac tcgcaatgcg caacggtctg tctgcgacga aggtcgacgc atacaacaaa
      361 caaaatgtcc tggccatcgt cctttcggtc gatccgcagt acctgacgca gcgcggcaac
      421 tcaccgctcc tcggcgtctg ggccagcacc aaaaagcgca tccccaagtg aggtcagcca
      481 tgacaaagaa actttttgca tctgtgcttc tccttgctgg cttgaccagt gtgggctgtg
      541 acaaaacggg cacaaatttc gaaaatgtcc cgtatgagtt cttcgatctc ggcaccggcg
      601 acggcggatc gtcactgatt cccggcaagc cgctcgccga cgcagtccag tatgatcgcg
      661 taggccgtgt gctggtctcg acgatgatgg tcaatccgtt tggcctcttc aaagacaccg
      721 cctacaccga cgaatacaag gatcgttaca acggggccgt accgctcaat ggctggctcc
      781 cgttttcggc ccagccgtac atccaggcca gtctcggcat ctgggacagt ctcgacgtaa
      841 actgcggcaa ccaaatcggc gccaatactc aggtgtcagc gacgcgctat cagaagctgg
      901 gtgatttgct ggccgacgat cgactctggg tcgataccac caagacttca tgcaaagtct
      961 atatgggcgt cgaacagaag ttcatcaatc cagcgacgcc agatgactgc ggtggccgac
     1021 ctctcgtcga aattccctca agtggcgtgg ccaagccgtt cgatgccgtg gatcagacgt
     1081 acaacctgct catcggcgca ggcgccacgg tcacagacgg ggtcgcgtct gactcagacg
     1141 gaaatcccgg cgggctgttc ccgttcctcg tcgacgccaa gtaacgtaca ctcaccgcag
     1201 tcctctgcgg caatctcgct cacgatggct ccagcgtctg tcgatctact gacggcgcag
     1261 attttttggt ttcgaccgct accgccgggt cttgtcccgg cttgagccgc agcgaaagcc
     1321 gcttgccttg acgtgacagc gtcacttttc ccgtcgacaa aagcttcccg agatttgcga
     1381 gcgacagcga aacgccatcg atggcaaatg ggacaaccag ccgacagttg cggtaatcac
     1441 cacagcccca ggcctgcttg ccccaaatca tcgacgccga ctggcagcgc ggacagcaga
     1501 cagtgggcac aagcggactc cccggcgaag gccgcgacga agcagcgctc gaccgacgcg
     1561 gtttcgcgac agaatccagt cgggatttcc gcaccgtgtc acgacgcaca gccttttcag
     1621 acgacgatcg ggtcgcggaa gatgaagcgg cacgcgagcg ccgcctgtgc gacttcgctg
     1681 gagcccgcac tggtgcatcg gttggcagcg cgacagccgc cggaggaggc agcccacgca
     1741 ctttgccaat gaactgctcg acgtaggaag cgatccccga cataaagtcc gtcgcgcgat
     1801 cctcgccgcg cgccatccga ttgagccgcg cttcccactg tcccgtcagt tccgccgacg
     1861 ccaagctcgg gtccggcagc ttttcgataa ggtcgatccc gagcggagtc ggccacagct
     1921 gtttgccacg tcgctcgatg tattgacgag aaattagtgt ttcgatcact gccgcgcgcg
     1981 tcgctggcgt gcccagcccc gaatctttca tcgctcggcg cagcgcttcg tcggatagct
     2041 gcttgcccgc actctccatc gccgacaaaa gcgccgcttc gctaaaccgc ggcggagctt
     2101 ttgtctgttt cttctgcacg acgaaagccc cgaccagcac atcgcctttg ccaagccgcg
     2161 gcagcgcctg ctggaccatg ctgtcatcgc cttcgtcatc gcttcccaag tcgtccccgc
     2221 cggacttgcg ccgcgaagga ccctcgccat caaggccggc cacatcctgc cagcccgcaa
     2281 ccagtcgctg ccgtccctgt gccacatagc gatccggcgg cagcggcaaa gcacacagac
     2341 gtccgtgctc atccggttcc agcttgtctt tgggcgcatc gcccatcgcg tccacccgca
     2401 ccgtcacggt cgtctgcgca aactctgcat cgggaaaaaa cgccccgacg aagcggcgca
     2461 ccaccacatc gtgcagcttg cgttcgtccg gtggcagaga cccgatccgc tgcggcgtca
     2521 ggttcgtcgt cgttggaatg attgcgtgat ggtcggtgac gcgctgatcc tgaaacaccc
     2581 gcgacggcgg cgaaacccgt tccagctgcg caacaaatcc cgcataggtc tgcgattcag
     2641 cgatcgcagc aaacagacga ggaagctgcc caatcagatc ccgccccaga tagcgcgagt
     2701 cggtgcgcgg ataacttagc agcttgtgtt cttcgtatag cttctgcgcg acggacagcg
     2761 tgtgctgcgc cgaccagcca aagcggcggt tgcacgttct ttgcagcgaa gtcagatcga
     2821 acagcagcgg cggaggttcg cgcagacgct tttcttcgac cgaatcgacc acgacaggcg
     2881 ctggacggtc gcgcgcggcc agctcatcgg cgatcgtctg cgaggccagg cgccggagct
     2941 tgccaaactg ccactgcgcg acaaactgga ccgggtcgtt tttccgtcgc agctgtgcct
     3001 gcacttcgaa gtaatccgat ggaacaaagc ggcggatctg ctgttcgcgc aaaaccaaga
     3061 tccccagcgt cggggtttgc acccgtccga gcgaacacag cacactctgc tgaccgcgtc
     3121 gccacaccgt caccgcccgc gtcgcattca tccccaccag ccagtcggct tccgaacgac
     3181 agcgcgccgc gcgcgccaag tcatcaaacg cctggcccgg ttgcaaatgt gtcattccgt
     3241 gcccaatcgc ttgctcggtc agcgacgaaa tccacagccg ctcgatcggc aatcggcagc
     3301 ccgcgagttc ataacaaagt cggaaaatca gctcgccttc gcgaccggca tcacaggcat
     3361 tcaccaccga cgaaaacgaa cggtctttta gcagcgcacg cacgactttc cactgcttaa
     3421 atcccgaccg aatcggtcgc agcttaaacg gctgcggcaa aatcggcagc ttgtcgagtc
     3481 gccacggctt ccacgcaggg tcgtattcgt gcggttcgca cagctcaacc agatgcccaa
     3541 tgcaccacgt aatcacatag cgatcgctgg tcagtgcacc gtcgctacgc ccagtcacac
     3601 caagcacttt ggcgagatcg cgagcgacct gcggtttttc agccaaaatg agctttcgcg
     3661 tcgagctacc tgccacgttc tgtcctcgta aacagccgca agaccgtagc agcttccctt
     3721 tcacttcgcc aaaccagacc gcgtgtcagc gacgcagctg tgcagattcc caaccaagca
     3781 ctgtgccgcg cgaaaatggc gcgataatgg gcacttccgc gcgaagtttc ttgacactgc
     3841 gctcgggcaa acactaaaga taccggcagt tttgcaaaga cttgtttgga ggatcccttg
     3901 gcaaacgtcc cttccgcaga gaaacgcaac cgtcagcgcc agaagcgccg cgcccgtaac
     3961 ctgcagcacc ttgttccgat gcgcacccga gtcaagcgtg cgcgtaccac cctcgctgat
     4021 ggcaaagcct cgagcgagga gacccaagag atgattgttg ccgctctccg tcagctcgct
     4081 cgggctgctt cgaagggtgt gattcacaag cggaccgcgg cccgcaagat ttcccgtctg
     4141 accgtcgcga tgaacaaagc cgcagtggat cgcaaagcgg catcggctgc gacgccgaaa
     4201 aaagccgtca agcacaagac ggacgccaaa gctgctcagc cgaaggctgc tgaaaccaag
     4261 gctcccgcca aagccccggc caagaccgaa gccaaggctc ccgccaaagc ggctgagacc
     4321 aaggccccgg tgaaggctgc cgaaacgaag gctcccgcca aagcggctga gaccaaggcc
     4381 ccggccaaga aggctgccga cgacaaagcg gctgccccga aggcgccccg cgccaagaag
     4441 tcgtaaccag tccccgacgc tattcgatcc gcagcgtcgt ggtgcttggt tctccacgtc
     4501 gcggcatcat cgacgggccc tgccccgtag ttcccagctg accgttttcc agccgcagat
     4561 acacgccata cagcgtgtcg agtccgggct ttgctcgctt ggcgacaagc tcatgcacca
     4621 tcgacaccag ctcgcggttg agcggcgctt gtattccatg ctgcgcggcc cgtcgcacga
     4681 cctctccgtt taggtagtcg atctcaggct tgcgaccgcg ctcgatcgcc accgccatcg
     4741 aactgcgcat ccgacggaac ttaaggccaa tccccagcag cagcgagtgc ttgaaaaata
     4801 gcttcaacga cccgacgctg tgccgtttgt cttcgtcgga cagcgccagc ttggcaatgt
     4861 caatcgttcc agcgactttc gccagccgaa tgccctcgca gtcggcgacg gcgcacagct
     4921 cgcgccagac ttccaagacc agccttcgca caaaccgatg cgaaagcagc cgcccgagcg
     4981 tatcgccgcc caccgcaccc agcgtactcg tcgcacagtt gatcgccagc ttgctccagc
     5041 gaacccccgc cagatcgtgc accactttgg tcggaacccc acgacccaaa agagtcgcca
     5101 agcgggccag ctctgcatcg tctgatcgag gttttagccg cccaatttgg aagccccccg
     5161 acgaagtgcg cgccgaaaaa cccggttcga tcagcgtcgc accaaagcca acgacacagc
     5221 caacgactcg atcatcgccc accaccttcg ctgcatgcgt ttcgggcaag ccgttttgca
     5281 gacacagaat cggcgcattt tcatgcagaa ccggcgtcac ctcaccgagc acatccccga
     5341 gcgtcgtcag cttcgtcgcg acaagacaga catcatagcg accgctcagc tcccttgcgc
     5401 tccgaaccgc ctgcgcaggc cgcgcgcgcc actcttttcc gtcgatgtcg cggacgcgga
     5461 tgccttgctc gctgatgacg cgctcgattt caggattgcc cgtcaccacc gtcacgtcgc
     5521 agccagcccg gctcagctgg gccgccgcaa tgccaccaat gccaccacag cccacgagca
     5581 aaactttcgc ttccgttgcc atcgtgatgt gccttctttc cgccgctgcg agcgtgttta
     5641 ctgtgccgta gccggtccgt cagatcaagc gactctccga cgagaatgcc gccaaaattg
     5701 gcccaacaat cgatccagcc gaaaaaatcc tttccgtgtc gcaagctaga cgagcgaaaa
     5761 cgcatgcgct aaggtgcgcg tcatgcacga gaagcccctg ttctcgacct cggcggcttt
     5821 ttacgacgtg gacgggacgc tgatcaaaac caacatcgtc cactcgttcg cgtactacgc
     5881 gctcaaccaa ccaaccccgc tgcgctcggc gctgaagggc ctgcaaaccg cggcatccat
     5941 cccgtttttt gtcgtcgctg acaaggtttc ccgcaagctc tttaacgagt tcttttaccg
     6001 ctactacgcc gggcattccg aagatcgcct gatcatcctc gccgaagacc tcttcgatga
     6061 ggtgattcgc ccgagcattt tccccggtgt tcccgatctc atcgaggaat cccgtcgagc
     6121 cggcgttcgc caagtgctcg tctccggcgg tctcgacttt acgatctggc cgctcgcgcg
     6181 ctacctcggg attcccaaaa ccgacgtgat cgcaaaccgg ctcgagttca gcaaaggcta
     6241 tgcgaccggc cagctctgtc cgccgttcgt cggtggagca accaaagcgc acatcatgcg
     6301 cgactttgcc ggcaaaaacg gaattgattt gggcaagtcg tgggcttaca ccgacagcta
     6361 ttcggactat ccgatgctgg cagcggtggg tcgtccgacg gtgataaatc ccgatctgcg
     6421 tctcaaactg gtggcgcgat cttacgattg gccgatcatc gatctgcgct agctgccgct
     6481 acacaacgac ttgcagacgg cgacggaagt cgtggcttag ttcggaattg ggaccgagca
     6541 actgaaacag cgccgccatc gctttgcggg cgccgtcgtc gcgaaacttc cgatcgcggg
     6601 ccacaatcgc caacagattt tccagtgccg cagcaaaatc ggcctgcctg gcctgggccg
     6661 cagcgacgat aaagcgcgcc gcggaatcgc gctcattttg cgtcagccgt tccgtcgcca
     6721 aagcaaagcc accgtcttca tcagccgcca acagaaaatc gacgaccgat tggagttctt
     6781 cgccgcgatc cgattccaca ctccgcggat cgaccgctcg caacagctca ccgacttcgg
     6841 caagcgaggc atgcccagac aaaagcaggc tctctgccaa aagcacctgc gcccgactct
     6901 tgaccgatcc gtcgagccca ccatcgagaa gctggcgcag cgattgggcg gcttcctgcg
     6961 gcacgcccat cgcaagctgc gacgccgctt cactgacttc acgttccccc ggtttgggca
     7021 gcagacgcga aatgaacgaa tcgacaaagc gtccgtcgcg cgcgccgacg aactcggaca
     7081 ccacctggcc tttgtgaaac gcctttacgg cgggaattcc ggaaatccga aaacgtgcgg
     7141 ccagatcctg ctgttcgtcg gtgttgacct tcgccagcag cacttttccc gcttgcgccg
     7201 cgacgcactt ttccaaaatc ggggtcagca cgcgacaagg cccacaccac ggcgcccaaa
     7261 aatcgaccag aaccggaacc tcgtgcgagc gctctagcac cgacgcctca aacgacgagg
     7321 cagtggccgc aaacacaaac ggagagtcgt tttccatgcc gcgccttgta tcacaagcgc
     7381 aagcgaccaa aagggatcag ttcagcgtga cgacggcagc ctgaccagac ggatcgacga
     7441 ccgcaaccga acaaggctga agaaggcccg acccagtttc cgacagtaca tcgacgagcc
     7501 gccacaaacc ggcaaccgac ccgtcaggga catcggccac gacggcacgg tcatcgagcg
     7561 cagcccccgc caagcgagcg gtttcgccat cacagcacac ctcgccgccc ggctgggacg
     7621 caagggcgcg ggcaagcgcc gtcgtcggat cgtcgggaat gaatgactcc gcagcggaca
     7681 caagacgtgg cagcgccgga tcgatcccat cacgatcggc agtgagccac agcgcgaccg
     7741 cagcgtcgga tagctgcacc ggcgacagcg ccgggccgga tacctcgacg gcaagcacca
     7801 cacaggctgc ggcttgtccg gtttgcagaa gggtttcggc ttcggccagg gcgacaagac
     7861 ccgatgcgcg accgacgcac agcgcaagcc ctggtccccg cagtcgataa aagatcgaca
     7921 gttccccgat aggcgacgac ggcagcgtat aagcaaacag tcgcggactc ggctgaccct
     7981 gcagatacga cagatagtat tcctcgtcgg tcttgtgaca gccgtgcgcc gtgccgatga
     8041 cgatcgcaac atcatcgccg ttcacgtctc tgcgcaaggg cggatgggcc gacaaaagcc
     8101 gcgccaccgc cacaaccgat agcgcacaca gtcgatccat gcgcaaaagc ggccctcgca
     8161 gctcggcagc cacgccatcg ggaagctgcg aaagcagcga atccgaagaa tcaacgacga
     8221 gacgcgccgc accacgaatc accgtcatgg caggctcccc gcgtcggaag ctgaggcaat
     8281 caccaccgcg gcattttcgc caccaaagcc cgatgccgtc gaaaggacac agcgcaccgc
     8341 cgactcccgc ggtttgcctt gcaccagatc gagcgaaaat gcagggtcta cctcttcgag
     8401 tccgacggtc tgtggaattt tttgcgacga aagaacctgc accgccatca ccgcttcgag
     8461 aagtccagcc gcacccagcg tatgtccgac ggagcctttg atcgagttga ccggcaccgc
     8521 caggcatcga tcgcccaaga gctgctgaat cgcgcggcct tccatctggt cgttaaacgg
     8581 cgttcccgtc ccatggctgg acacaaaatc gaccgcatcc gtcgaaagct ccgcgtcgaa
     8641 gagcgcccgc tgcatcgcca gcagcgcgcc ccgtccctgc ggatcgggtg ccgtcatgtg
     8701 atgcgcgtct tgcgaaagtc cggcaccggc caaaaacgcc aaaatcggct gacctcgccg
     8761 tcgcgcgtgc gattcggttt ccaaaatcac acacgctgac gcttccccga gatttacgcc
     8821 gctgcgtcgc cgatcgaatg gtcgacaaac gtccgccgac tgcgcgcgca gggaacgaaa
     8881 accactgaca acaaacggcg aaagcacgtc gacaccaccg cagaccacga catcgcacag
     8941 cccacgtcgc agccacgaca aagacagcga cagcgcggta ttgcttgagc agcaggccgt
     9001 cgagactgca cgcacccgtt cggcacccag acgagtcgca agctgctcgg ccaacagccc
     9061 acagccatac gccagagacg gcggctcgac ttgcttgtgt ttggactcga tgccgacgag
     9121 ccacggcgac ttttccccga gcgtcgtcgc cacgcagacc gcaatccgcc gtccgtcgcc
     9181 agcgcccggt ccgccaagcc ccgcctgacg cagcgcgaac tgcgccgaat ggagcaaaaa
     9241 aagaccggcc cgcggctgtc ccgacaagat ggctccgccg tcgctgttgt cttcatctgg
     9301 aagcgatgca gacacgagcg gaccgacaga aagcgtcgtc cccacaagcg ccggcaaatg
     9361 ccgaaccgcc gtctgtcccg ccaagagccc cgcaaacagt gcatccggat tttcgccgag
     9421 tccacacagc gacccgagcc cagtgatggc gacccgctgc gccatcagtt ttcagcgctc
     9481 accgtcggtg gcagggaagc agacggagcg atgccatcca ccgggagcgg cacacggacg
     9541 tcgaagaaat cgaaaaactg ataccactgc gtcgggtact tgcagacgat cttttcgtag
     9601 atggccacca tcgcctcggt cgcgcgacgc acgtcggctt cacgatcgcg ggtgttttcg
     9661 acgatgactg ccggcccgac gtggtgaatc agccgcggcc tgcccaggct ttcgtcgatg
     9721 acaaagaagc tcggaatcag cggagcccct gtcacccgcg ccaaaagcgc cggccccatc
     9781 ggaaagcgtg cgtctttgcc acacatcttc accgtcaggc ccgtgtcaga caggacgcga
     9841 tcgatctgca tcccgaccac cgatttttcg cgcagcacct tcgcaatgct cagcgcggaa
     9901 aagggcgatc cggtcgtata aatgaccttg aagcgatccc gaaagcgctg ctcccatttt
     9961 tgcacgagcg gattcggctc ttcggcgcga gcgacgtaaa acggcggcaa atcgcgccat
    10021 tcggccaaaa acggtccaac ctgccacatt ccgatgtgac ctgttgccat gattccgcct
    10081 ttttcgcgga tcaggctcac cagctcgggc gtccgaccga tgctctcgac ttccatcgta
    10141 agcggctgcc ccaaatggac cttgtagctg tcggtgagca tctgcgcata gttgcgaaac
    10201 gtgacgagtc ctctccggtg cgtctgccaa aaacccactt tgccagccac cgtttccaga
    10261 ttgcgcaaca ccgcttcgcg cgctttgcgg atgaggaaat agaaaatccc gccccacatc
    10321 ggcatcgtca ggcgctggag ccacgtcgga atgtgcttta tcccgcccag catgatgcgg
    10381 cggtaaaatg gcccttcgta gcggtgctgg agcgaaagtg cagacttcga ggaatcgttc
    10441 gacatcggct tagtagcagc ggcccaccct ctcgaaaccg ctgagtggat gacaagtccc
    10501 aagaccggca ttgcacatca cttcggtgcc accgtcggga ggcgagccgc cgtcgggggg
    10561 cgtcggcagc gtgcagtacg gacgacaagc ggagtcacgc ccgctgcgcg tggtgcaggc
    10621 aaagccgctc tgacagttcc acgagcgctc acacggcgcg tccaagtcgc cgctcgagtc
    10681 gccgtagcgc tgacgagacc agcagaccgt gccggtgcct ttgtcgtcgc ccaggacgta
    10741 acacgaaagc ggcgtgccgt ctttggcttt gccgcagccg gtttgaggaa agaccggctc
    10801 acagttgtca ggggcattac agaaaccaac accggtcact ggggacggaa gaacgcacag
    10861 atccgacttg ccacagcgcc cacgagtccg cagcgcacac agccgtttgc agcggttcgt
    10921 tccgtcggca gtcgcgacgc agccaagccc actgccgcag cggtcgtaac gccgagccgg
    10981 attggtcgca gggctggcca ccttcgcgag agcacactct tggttttctt ttagcgccgc
    11041 agcatcgtcg ggaacacact gggtcagata cccttccgcg ccagtttctc cgacgagaac
    11101 aatgccgcag tgcagcccgc tcgcgcagcg cacatcaatc ggttcaacac cgtcgggcga
    11161 gcagtcctcg aatacgccaa cggtcttgac cgtgataccc aagtcttcga cgagcggtgg
    11221 caacgcagaa tcgacaaccg gcggcgagtc atcggtctgc ggacaacccg acagagcgac
    11281 gacgagcgat agcaaaagcg gcaccgtgtt gcgttttatc gtccgcagca acgcatggga
    11341 aaatgacatg gtgactaccc aatcctcggg acgcacatgt tggcgacgca gcggaactcg
    11401 ctggggcatt ctccgtcggt ggcacagacc acatcggtgg tccaaatcgc agagtgattg
    11461 cccgtcgacg gttcttggaa cggcagccaa atcggctggt agctcggatc ttgggcgccg
    11521 gtccgcgcct tgtcgagatc gagtccgaac atgaaaagct gtggctggct gccgccggaa
    11581 aacccgtact tgtagcgcgc cgagtagacg atgaacgaga tgcgcccgtt ctgcaaaaac
    11641 ggcgcaaact tcggccagtt gttgctgttg ttcggcgcgt gcgtccccgc cagaagctct
    11701 atcggcgtgc cgttgtccac cggctggccc gcgtcgtcga tggctttgac cagccgcagc
    11761 cgcgtctgca ccgcgttgta ctgctggcac gacccggagc aggtctggga attgaacacc
    11821 agccacttgc tgtcgggcga ccagcttggc caaaagtgca cttcgctgcc caccttcgcc
    11881 ggaatgacgg tggtggcctg gccaaacgaa ccgctgttgt acggcatgac gacgatgtcg
    11941 ccgctgtcga ccagctcgaa gtcgtagctc gacgcacggg tgtagcgcac aaacgccagc
    12001 catttgccgt caggggacca ctcgggcatg gcgccaagtc ccatcccacc aatctgcgcg
    12061 ggctgcaccg tttcgagcac cgcaccgctg ccagcgtcgc gcactttcag cgtgccgctc
    12121 cagttggtga tcagcttgtc ggcgcctggg ttaaaccaag cgaagttcca cgcgatgctc
    12181 gagctcagcg ggaacgcgcg cattttgggc atgattccgt cgacgacagt agagccagtc
    12241 cgggtctgtc ccgagccaaa ctcgacggca acttttttgc catcgcgcga caccgcatgg
    12301 cagccggcgc atcccgtctc atcgccattg gtgatgaaat cgaccggctt gctcgcaccg
    12361 atgggcaccc gcttgatgcc gcggatcgtc ggtgaaaagt aataaagtcc gcctttgatg
    12421 tcttcggggg caaagctcac cgtcgccggc atcgacgttc catacaccgc gcctggaccc
    12481 gacgaacccg agacgacaat ttggacatct tggccctgcg ccgagtgacc gaccgcgctc
    12541 cacagcttgt cgtcgatgcg gtacgtgcac tgcgacgacg gacacaggct cgaaccaacg
    12601 tagaacgacc gctcgtaggt cgccccgatg acctgcacgc ggaacacttt ctggcccgcg
    12661 gcggcctgcc actgcatcgc cagctgcagg acgttggcag ccatcatcgt tttgtcaaac
    12721 gggtacgcga tgcttggcgc cgggccaccg ctcgatcccg aaaagtagtc tttggcatcg
    12781 ggtggtgccg tcgaatcaac ctcgtcggga gcaatcagct tgataagcag cggtgccgac
    12841 gccgtagagc cgcgcagact cgcgaagact ttgtacgatc cacccttttg cagcgtcggc
    12901 tggatcgtca gcttgccctt gacgatgctc ccgacgctgc tgtccgacac cgtccaggtc
    12961 gccaggttgg tgacgtcgcc gagcttgctc gacagcgcgc ggaagttcac catctgggcg
    13021 ctgccattta tcgtgatcga ggcgtttccg ggcgaaatcg tgatcgtgcc cggtttcttt
    13081 tcttcgtcgc tcgggtccat catcgagttg ccagaatcgg tatcgattga ctcacgcact
    13141 ggcgtgcaat cactcgcggc aagcagcgca gtcagcgata gcagcccccc ggcgcgacgg
    13201 agcgagggcg aaaccgcggt cggaaacgac atgacgacct cctgtttgga ggtgccagcc
    13261 tagcgagaat ggagcaaagg gacaataagc tctcaatctt cgcgatggaa acacggcctc
    13321 aaggtgagcg tgcgaggaag attcggcggg gaaaacggcg cgaaaagccg tcccaaaagc
    13381 gggattactt gcgacgggcg ggcttggccg gagccgcttc agccggcgct tttttggcgc
    13441 tcgctttggg ctcggctttt ttgtcggact tgtcagccgc tttgccatct gcagcggcag
    13501 gcttcggctt tttcgctggt tccgcagcgg caggcgcttt ttcggccttc gccggcgctt
    13561 tttcggcctt cgcgggtgct ttttcggctt tcgc
//
LOCUS       DAORGN010000138        13513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1889_length_13513_cov_5.955343, whole genome shotgun sequence.
ACCESSION   DAORGN010000138 DAORGN010000000
VERSION     DAORGN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_13513_cov_5.955343"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKL17_14595"
     CDS             complement(<1..450)
                     /locus_tag="PKL17_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46013.1"
                     /translation="MLSTGSDPKALRAKLLEYLGNVKQFFEATNVSFNLNASPYWSAF
                     DALRRNKPLAEEFVTQLTTLLEFLDRELAPALLQAQNQVPSAPVASAPVGPWPGMPLP
                     GGPSAAMPGINSDETQILDRVSPAHMIQRISSSDPVPRMPAPTPGMTH"
     gene            complement(556..1140)
                     /gene="rsmD"
                     /locus_tag="PKL17_14600"
     CDS             complement(556..1140)
                     /gene="rsmD"
                     /locus_tag="PKL17_14600"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNK46014.1"
                     /translation="MIRIVAGELGGRRLRTPAGLSTRPTSERVRESLFNILGPIPDGA
                     IVWDLCAGSGALGIEALSRGAGQALFVDQDASACRVLRRNLQDLGLVDRSKVIEAPLG
                     RFLASQQRTSSGVDLLLADPPYASGELARLLDFLAQHGAACLRMGAQVVIETDVRQKG
                     ELAIGARRGPLLCTDVRRYGDTVLLFWQRSADCA"
     gene            complement(1156..2898)
                     /locus_tag="PKL17_14605"
     CDS             complement(1156..2898)
                     /locus_tag="PKL17_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNK46015.1"
                     /translation="MQSASMELSGSTQSRTATSQAELDKYLAILSENATPFAKLPPRE
                     KAALLRACVPRLQSVAKDWVAAACQAKGLRVDSPAAGEEWLGGPYVTMRCLRKLIVAL
                     EDIARVGKPQLPSGAIQLARDGRTEVLVMPGEAYDHAISAGFTCRLRMLAGYDDRRVR
                     EEQAIFYSKKDPQGGVSLILGAGNVASIPPTDALYKMFHEGKLCIVKLNPVNEYLGPF
                     YEKAFQPLVERGYLRFVYGGAEVGSYLSYHPQIVDVHITGSDRTHDLIVWGPPGDERV
                     RRQAANDPLLKKNITSELGCVTPVMIVPGNYSASELRMLAENIATQVVNNGSFNCNAA
                     KILVVSESWPQRQELLKQIQEIFQEMPTRRAYYPGAQERFAKLTQGKTVTRLGRAGDG
                     ELPWTLIRDVSPKTTDEPLFSTEPFCSIISEVALPEKDAAEFVTAAARFANDRLWGTL
                     SCSMYIAPSTEKQPEVAAAVEKACDVLRYGVVAINHWPAVAYGAMTPPWGGHPSATLL
                     DIQSGLGYVHNSFMFEGIEKAILRGPLVPSPKPPWFVTHKRSHEVAQRMVQFEAEPSW
                     LKIPGIAMQALLGS"
     gene            complement(2957..4411)
                     /locus_tag="PKL17_14610"
     CDS             complement(2957..4411)
                     /locus_tag="PKL17_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK46016.1"
                     /translation="MKTPKTAIPQLRSYLAARSLLFVLGGAVASCSNPSDSLIPNDMG
                     GVGNISITSVSPAVVGIAGGTPVAVYGTGFAAGATVAFGNKLATQVQVISSTQIQMLA
                     PAAENGPGAVPVTVRNADGSFAVATGLFRYVRVVLDFDNTRVPVGTQPVAVIAEDTNG
                     DGNPEIITANDGTANVSVLPANLNYQNGSTYPTPVGPTALVVADFNGNRIPDIAIACN
                     NGSAQDLAILSGNGGGGFMSPVTFTVGRNPTAIASRDLDGDGKPDIVMAPRTGDTVYV
                     LRNTSVTPGIAFTTGATYNLKASSAPSALLLDDLTGDAYPELVTGNYSDASISTFIGN
                     SGGIFSGPKNTTVVAQVLSLAAGDLTGDGKKDVATLSYGGRSISILKGKGDGTFDPGD
                     TLSTEKDPRMLAIADVDGDGKLDLVVANSGTDSVGIYLGNGSGGFDAAQTITVGSTPY
                     ALVIKDLNLDGKPDIVTANFNSNDVSILRNKTQR"
     gene            4686..5996
                     /locus_tag="PKL17_14615"
     CDS             4686..5996
                     /locus_tag="PKL17_14615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNK46017.1"
                     /translation="MANVVIVGAQWGDEGKGKVVDLYTEFADMVVRFGGGANAGHTLV
                     VGGQKLVTHLVPSGVLRGKGKRCVLGDGMVIDPQTLLEEMSELRSRGLLVDDRDLVIG
                     ERAHVILPSHKLLDGLREQGKGALGTTRRGIGPAYEAKMARRGVRMRDLLSADRLRAR
                     IAHQLEETAPQIRHLGGTPPDVEAMTDQYLGFGERLRPHIHDATRVLSEEMKRGQHVL
                     FEGAQGALLDIDHGTYPYVTSSTTLAAGACVSCGVGPTAISSVIGIAKAYTTRVGAGP
                     FPTELTGELGEQLRQAGAEFGATTGRPRRCGWLDIAALRLAVRLNGIAGIALTKLDVL
                     AGQRKIKVCVGYKLPALAGGSGVSETLDELPLDPEDIARAVPIYEELDGWDAELRNVR
                     EVEDLPPGAKRYVRRIETLLGIDCCLISVGPGRAETIMLKNPFR"
     gene            6153..7919
                     /locus_tag="PKL17_14620"
     CDS             6153..7919
                     /locus_tag="PKL17_14620"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNK46018.1"
                     /translation="MKIVCDNCATKYQIADEKVSGKAFKIRCKKCGHVIVVGKNDGSA
                     SAPAGGDAPAEAPAAASEPAAGDAVWHLVIDREQVGPMTAEEVRGKVKAGQVDSETYG
                     WKEGFEDWLKLSAIDDFKELFAGKPSDQATKRVDPAEQQAQAQPAATPGADLMGGQAA
                     SEPVKTAAPTAATLYSEGGSNDARAQLQAAQAAREAQIAAQQAAQAQQAAQAQQAAQA
                     QQAGDAGGATGMTGARSENSVLFSLNNLSALAGGGGGGNASAAAPKPGYASGQSEASG
                     LIDIRAMAAQHLGGPMSGANVNKAPDLFGGGDAAPVFAPVAPAVLVPTAPSEGAPKWV
                     WGLVAVGGVSIIGLIVFLVVFLSGPSTPQVATGPTTGTNPNTGTATGAGTTGTAPNPG
                     TTAATAPTENKTTKTETAAKTETAAKTESGSKDSGSSKSSKSSKSEKGDKGSKAAAAP
                     KEAAAPPEKAGPGKAPEEAAPPPKKAAEKPKAAKDDLDSLLDSASSGSGGKPQKAEKK
                     DVPENLDRGTIMSTLKSANFAPCKAEGASGVVNVKLTIGRNGKVSDASGPNDCVVKVV
                     KGVKFPEFSGDPMTLTYPALIR"
     gene            8102..8174
                     /locus_tag="PKL17_14625"
     tRNA            8102..8174
                     /locus_tag="PKL17_14625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8135..8137,aa:Lys,seq:ttt)
     gene            complement(8252..8494)
                     /locus_tag="PKL17_14630"
     CDS             complement(8252..8494)
                     /locus_tag="PKL17_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46019.1"
                     /translation="MPRWFLIVNGVALLTMGVGLLALRLRERPLYKHVLGLLWAALCC
                     VAGVVLLLMAQGHIVQPGAERPARPHNVAPEFPQGQ"
     gene            complement(8506..10086)
                     /gene="cysS"
                     /locus_tag="PKL17_14635"
     CDS             complement(8506..10086)
                     /gene="cysS"
                     /locus_tag="PKL17_14635"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNK46020.1"
                     /translation="MGNGSTSLAKVSIYDTFAQRKVEICPLLPGEIRMYVCGPTVYNL
                     FHVGNARPLVAFDVVARHLRARGYRVKFVRNITDVDDKIIARARECGEDPKAFAERWT
                     AEYHRDFASLGCQPSDVEPRATEHIGEMIAHIEKLIAHGLAYESDGSVYYSVASFAAY
                     GELSRLPREELLCGARKDIEPGKRDAADFALWKSAKPDEPSWPSPWGAGRPGWHIECS
                     AMSEKYLGPTFDIHGGGIDLRFPHHENERAQSQGVHGPGTFARYWMHNGFIGFRWVYG
                     EKLIAEGSKIAKSDEAMRKLYHMFVARNCIDRHGGEAVRLWLLTTQYRNPLAFDLDMN
                     PDDPAETAPFRLPGLEEAEKRVEYGYQTKQRLEDALHGMKPEPPGVVLPEAEGWLERL
                     YLALDDDFNTPVALAEWSFALSLVNRLLDGKITPPPAKDVRRRTLFRLAADLDRAGAV
                     LGLLERPAADYLLEHRRRRIAVRGLDAAQIEAQLAERTQARSSKDFAKSDALRQSLWE
                     LGVEVMDTPTGTRWRVRD"
     gene            10328..12385
                     /gene="dnaG"
                     /locus_tag="PKL17_14640"
     CDS             10328..12385
                     /gene="dnaG"
                     /locus_tag="PKL17_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNK46021.1"
                     /translation="MGLIPHSKVAEIRDRTDIVAVIGEHVQLRRAGVNYLGLCPFHGE
                     KTPSFNVSATKQFFYCFGCQKSGDVIRFLMEREGRSFTDVVRDLAARAGIDIPDDEEE
                     SRPERKAQRLAQESERLRLLKLNALAARYYEAQLAVNERALAYVASRGIGDDIRKSFR
                     LGYAPDSWDGLLQVLRQRGVPHELAELGGLLIRRDGAPALPKGAPPTPQTHYDRFRDR
                     LLCPLLLPAQSKQTDCDVIAFSGRLMPTDTPRPNEGAKYINSPETPLYRKGQNLYGLH
                     CARDVVRQRRQVILVEGNFDVLSLHQHGFGNTLAPMGTALTETQVRLLGRLLGEDGHV
                     VLMLDGDKAGRAATMKDISLLMLTQAQLSDVASLSQRDIDVRVAKLPDGEDPDTLANR
                     DAAALLRCVAKAQPAIDYVLDEVLAGSESDSLTGRAKIISRMTPLLASLRNETLRELY
                     VGKLAMALAVDAKLLWKQIEQAKTSGPATVEPATTPPTVRSFDPPRNASQPRSQAPTQ
                     ESPTRRPQGRAAAGMPFDGHLRNLLGLLADHPALWDQLGDDTLTAIPNPVLSELLRDA
                     RDLCHSGEIVSVETILNMCPVELRAPVAEAMLQNRFKESQHPDEELSRISISLRVQAI
                     AREVADLQQTFQRQSRSGEQENRLQLLSRIQDLTKLRASLLQNSHPAQAAVAGTAQGD
                     TPR"
     gene            12382..>13513
                     /locus_tag="PKL17_14645"
     CDS             12382..>13513
                     /locus_tag="PKL17_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNK46022.1"
                     /translation="MNRDNAERRKQQLITMGKAKGYLTYDEVNDHMPDDVVSSDQIDD
                     WLSSLGDEGIEVVDSAASVKVRSNTAPKVPELIEDDEKKEEEEEEEFDGGYSKTNDPV
                     RMYLRKMGSVSLLTREGEVEIAKRIEEGERRVLQVVLNSSIAVEEILDLGERLKKQKI
                     RVKDVVRDLDEEDADFDEEWHTERVIKIIDKVRRLQRENEKILEELEIPQTTGPASAT
                     PTPPPPAPSKAVEAKRKKLRDKIEANKSEMFELLSELRINKKVIDRIVQKLKSLVLRI
                     EKGEAELEEFERRVGMPAKELAKTLKEMKANATKAKAITKKLGVTAEELESAALGMAE
                     AKKRIKKIEEEAKLSVDDLRATYKEIHEGERMAEKAKAELVEA"
BASE COUNT         2794 a         4192 c         4091 g         2436 t
ORIGIN      
        1 gtgagtcatt cccggcgtcg gtgcaggcat ccgcggcaca gggtccgagc tggaaatcct
       61 ctgaatcatg tgcgccggcg acacccgatc gaggatctgt gtctcgtcgc tgttaatccc
      121 cggcatcgct gcgctcggcc cacccggtag cggcatccct ggccacggtc ccacgggtgc
      181 cgacgcaacc ggcgccgacg gaacttggtt ttgtgcttgc agcagcgccg gcgccaactc
      241 gcgatcgagg aactcgagca gcgtcgtaag ctgtgtcaca aactcctccg ccagcggttt
      301 gttccgacgg agcgcatcaa acgccgacca gtacggcgag gcattcaggt taaaagagac
      361 gttggtcgcc tcgaagaact gtttgacgtt gcccaggtac tcaagcagct tggcccgcag
      421 ggccttggga tcagagcctg tagagagcat tgtctcttag gttctcgcaa cttgcgaaat
      481 tgcgcaaggt tgccgctctc gacgacgcgg aggcggaaac acggtcaacg cgaacgcgcc
      541 gccgaagtcc ccaaatcacg cacaatccgc gctgcgctgc caaaacaaga gcacggtatc
      601 gccgtatcga cgcacatcgg tgcaaagcag cggaccccgc cttgccccaa tcgccagctc
      661 gcctttttga cgcacatcgg tctcgatgac gacctgggcc cccatgcgca gacacgccgc
      721 accatgctgg gccaaaaagt cgagcagccg tgccagttct cccgacgcat acggcggatc
      781 ggccaaaagc aaatcgaccc cgctcgacgt tcgctgctgg ctggccaaaa agcgtccgag
      841 cggtgcctcg atcactttcg agcgatccac aagcccaaga tcctgcaggt tccgccgcag
      901 cacccgacac gccgacgcat cttggtcgac gaacagcgcc tgcccggccc cacgcgacag
      961 cgcctcgatc cccagcgccc cactgcccgc gcacagatcc cacacaatcg caccgtcggg
     1021 aatcgggccc aagatattga acagcgattc acgcacccgc tcggacgtcg gacgggtcga
     1081 aaggcccgcc ggcgtccgca gccgacgccc tcccagctca ccagcgacga tacgaatcat
     1141 gatgtggcga caagcttagg aacccagaag ggcctgcatc gcgatgcccg gaatcttcag
     1201 ccacgacggc tcagcctcga actgcaccat ccgctgggcc acttcgtgcg accgcttgtg
     1261 ggtgacaaac cacggcggct tcggcgacgg aaccaggggt ccacgcagga tcgctttttc
     1321 aatcccctcg aacataaacg agttgtggac gtatccgagc ccgctctgga tgtcgagcag
     1381 cgtcgccgac ggatggccac cccacggcgg cgtcatcgcg ccatacgcca ccgctggcca
     1441 gtggtttatc gccaccacgc catagcgcag cacatcgcag gctttttcga ccgctgccgc
     1501 cacttccggc tgtttttccg tcgaaggcgc gatgtacatg ctgcagctga gcgtgcccca
     1561 cagcctgtcg ttggcaaagc gggccgccgc ggtcacaaac tccgccgcgt ccttttcagg
     1621 cagcgccact tcactgatga tggagcagaa cggctcggtc gaaaacagcg gctcgtcggt
     1681 ggtcttggga ctgacatcgc gaatcagcgt ccacggcagc tcaccgtccc cagcccgtcc
     1741 cagcctcgtc accgtcttgc cttgcgtcag cttggcaaag cgctcttgcg cccccggata
     1801 gtaggcccgt cgcgtcggca tctcctggaa tatctcttgg atctgcttga gcagttcctg
     1861 ccgctgcggc cacgactccg acacgaccag tatttttgcc gcgttgcagt tgaacgatcc
     1921 gttgttcacc acctgcgtcg cgatgttttc ggccagcatc cgcagctccg acgcggaata
     1981 attgcccggc acgatcatca ctggcgtcac gcaaccaagc tcgctggtga tgtttttctt
     2041 tagcagcgga tcatttgcgg cttgccgacg cacgcgctcg tcacccggcg gaccccacac
     2101 gatcagatcg tgggtgcgat ccgaacccgt gatgtgcaca tcgacgatct gcggatgata
     2161 cgaaaggtag ctgccaactt ccgcaccacc gtaaacgaag cgcagatacc cacgctcgac
     2221 gagcggctgg aacgcttttt cgtaaaacgg ccccaggtat tcgttcaccg ggttgagctt
     2281 gacgatgcag agcttgccct cgtggaacat cttgtaaagc gcgtccgtcg gtggaatcga
     2341 ggccacattg cccgcgccaa gaatcagcga cacgccgcct tgcggatctt ttttgctgta
     2401 gaagatcgcc tgttcttcgc gaactcgtcg atcgtcgtag ccggccagca tccgcagccg
     2461 acaggtaaaa cccgccgaaa tcgcgtgatc gtaggcttcc cccggcatca ccagcacttc
     2521 ggtgcgaccg tcgcgagcca gctgaatcgc gcccgatgga agctgcggtt tgccgacgcg
     2581 cgcaatgtct tccagcgcca cgatcagctt gcgcagacag cgcatcgtca catacggacc
     2641 gccgagccac tcctcgcctg ccgctggaga atcgacccgc agacctttgg cctggcacgc
     2701 cgccgccacc cagtcttttg ccaccgactg aagccgtggc acacaagccc gcaaaagcgc
     2761 cgctttttca cgcggcggga gcttggcaaa cggggtcgca ttttcactca gaatcgccag
     2821 gtacttgtcg agttccgctt ggctcgtcgc tgtccgactc tgcgtactac cgctcaattc
     2881 catcgatgcc gattgcatga attactccta ccacagactg cgttgcgcgg ggattcggac
     2941 gagcagcctg tctgcgctag cgctgcgttt tgttgcgcag aatcgacacg tcgttgctgt
     3001 taaagttcgc ggtcacaatg tcgggttttc cgtcgaggtt gagatccttg atgaccagtg
     3061 cgtaaggcgt cgatcccacc gtaatcgtct gcgcggcatc aaacccgccc gagccattgc
     3121 ccaggtaaat gccgacgctg tccgtgccgc tgttggccac cacaagatcc agcttgccgt
     3181 cgccatccac gtccgcaatc gccagcatgc gcgggtcttt ttcggtcgaa agcgtatcgc
     3241 ccggatcgaa ggttccgtcg cctttgccct tcaaaattga gatcgatcga ccaccgtagc
     3301 ttaaggtggc cacgtctttt ttgccgtctc cggtcaagtc tcccgccgcc aacgacaaca
     3361 cctgcgccac caccgtcgtg ttcttgggtc cggaaaagat gccgccgctg ttgccgatga
     3421 acgtcgaaat cgaagcgtcg ctgtagttgc cggtgacaag ctctggatac gcatcgccgg
     3481 tcaggtcgtc gagcagcagc gccgacgggg cactcgacgc ctttaggttg tacgtcgccc
     3541 cggtggtaaa ggcgattcca ggcgtgacgc tggtgttgcg cagaacatag acggtgtcgc
     3601 cagtgcgcgg agccatcaca atgtccggct tgccgtcgcc atccaagtca cgagaagcga
     3661 tcgccgttgg gttgcgaccg acggtgaacg tgaccgggct catgaaaccg ccgccgccgt
     3721 tgccgctcag gatcgccagg tcttgcgcgc tgccgttatt gcacgcgatg gcgatatcgg
     3781 ggatgcgatt gccgttaaag tccgccacca caagtgccgt cggaccgacc ggcgtcggat
     3841 acgtcgaccc attttgatag ttcaagttcg ccggcagcac cgatacgttg gccgtaccgt
     3901 cgttggcggt gatgatttcc gggttcccgt cgccatttgt gtcttcagca atcaccgcga
     3961 ccggctgcgt cccgactgga acgcgcgtgt tgtcaaagtc gagcacaacc cgcacataac
     4021 ggaaaagccc cgtcgcgacc gcgaagctgc cgtccgcgtt gcgcaccgtc accggcaccg
     4081 ctcccggacc attttccgcc gccggtgcca gcatctgaat ctgggtcgaa gaaatcacct
     4141 gcacttgcgt cgccagtttg ttgccaaatg cgaccgtcgc accggcggca aagcctgttc
     4201 cgtacacggc gaccggggtg ccgccggcaa tgccgaccac ggcgggactc acgctggtga
     4261 tggaaatgtt gccgacgccg cccatgtcat tggggatcag cgaatcggat ggatttgagc
     4321 agctagcgac tgcgccgccg agcacaaaca aaagggagcg ggccgcgaga taacttctca
     4381 attgaggaat tgcagttttt ggagttttca tagcttttcc gtctccttgc aaacgctttt
     4441 ttctatgagt aaggcacatg agtatgggat cacgatcccg gcgagacaag ggtggcccgt
     4501 gagataattc acaaaaacag atgtgtttcc cccgcagctg attgcagccg ccgtgtcaag
     4561 ctgggcaaag tcgcgaggac aaaaggcatc gcttgcattc gggtgcgcgg atcgcctagc
     4621 gtgggtcgct tgagaggacg ccgcttcgca cctttcgcaa ggtcgggcac ttaggacgag
     4681 cacagatggc caatgtggtg attgtcggag cccaatgggg cgacgaaggc aaaggcaagg
     4741 tcgttgacct ttataccgag ttcgcagaca tggtcgtgcg cttcggtggc ggggccaatg
     4801 ccggccatac cttggtggtg ggtggtcaaa aactggtgac ccacttggtt ccgtcgggcg
     4861 tgctgcgcgg caaaggcaag cgctgcgtgc tcggcgatgg aatggtgatc gatccgcaga
     4921 cgctgctcga agaaatgtca gagctgcgga gccgtggctt gctcgtcgac gaccgtgatc
     4981 tggtgatcgg cgagcgggcc cacgtcatcc tgccgagtca caaactgctc gacggactgc
     5041 gagaacaagg caaaggcgcg ctgggcacca ctcgacgggg aatcgggcca gcgtatgaag
     5101 ccaaaatggc gcgtcgcggt gtgcggatgc gcgatctgct gtccgcagat cggctgcgcg
     5161 cgcggattgc ccaccagctc gaagaaaccg ctccgcagat acgccacctc ggcggcacac
     5221 cacccgatgt cgaggcgatg accgatcagt atctcggctt cggggaacgc ctgcgccccc
     5281 acattcacga tgcgacccgc gtgctcagcg aagaaatgaa gcgcggccag catgttcttt
     5341 ttgaaggtgc acaaggcgcg ctgctcgaca tcgatcacgg cacctatccg tatgtcacgt
     5401 cgtcgaccac gctcgcagcg ggcgcctgcg taagctgcgg cgttggaccg accgcgatct
     5461 cgtcagtgat tggcattgcc aaggcctaca ccacccgggt gggcgcaggt ccgttcccga
     5521 cagaactgac gggtgagctt ggcgaacagc ttcgacaggc cggcgccgaa tttggcgcaa
     5581 ccaccggacg tccgcgtcgc tgtggatggc tggacattgc ggcgctgcgg ctggccgttc
     5641 gcttaaatgg catcgccggc atcgccctca ccaagctcga tgtgctcgct ggccagcgca
     5701 agatcaaggt ctgtgttggc tacaagctgc cggcgctcgc gggtggcagc ggggtgagcg
     5761 aaacgctcga tgaactgccg ctcgatccag aagacatcgc gcgcgcagta ccgatctacg
     5821 aagaactcga cggctgggat gccgagctgc gaaatgttcg cgaggtcgaa gaccttccgc
     5881 ccggtgccaa gcgttatgtg cgccgtatcg agaccttgtt aggaatcgac tgctgcctga
     5941 tctctgtagg gccaggtcgg gcagaaacca tcatgctcaa gaacccgttt aggtagcgat
     6001 ggacttgccg caaacaactc gtttggccgt gatttccggc caggtttttc gggggagcca
     6061 agcgtgaacc gtgcttgcgg cgaaaagcgc gcttcgtata agctgggctt cattggtcac
     6121 cgcttcgagc ccgaagaggg gatttttcca acatgaaaat cgtctgcgac aactgcgcaa
     6181 ccaagtacca gattgccgac gaaaaggtca gcggcaaagc gttcaagatt cgctgcaaga
     6241 aatgcggcca tgtcatcgtc gtcggaaaga acgacggctc agcgtctgct ccagcaggcg
     6301 gtgatgcgcc cgccgaagct ccggcagctg cgtctgaacc cgcggctggc gatgcggtgt
     6361 ggcacctcgt gatcgatcgc gaacaagtcg gcccaatgac cgccgaagaa gtccgcggca
     6421 aggtcaaagc cggccaagtc gattccgaga catatggctg gaaggaaggc ttcgaggatt
     6481 ggctcaagct ctccgccatc gacgacttca aggagctttt cgccggcaag ccgagcgacc
     6541 aagcgaccaa gcgggtcgat cctgcggagc agcaagccca ggcccagcca gcagcgaccc
     6601 caggtgccga tctgatgggc ggacaagccg ccagtgagcc agtcaagacg gcggccccga
     6661 cggcagcgac tctttattct gagggcggaa gcaacgacgc ccgcgcccag ctgcaggccg
     6721 cacaggccgc gcgtgaagcc cagatcgcgg cccagcaagc agcgcaagcc cagcaggccg
     6781 cacaggctca gcaggctgca caagcgcagc aggcaggtga tgcgggtggc gcgacgggca
     6841 tgacgggtgc tcgcagtgaa aactcggtgc tgttctcgct gaacaacctc tcggcgctcg
     6901 ccggtggcgg tggcggtgga aatgccagcg cagccgcacc gaagccgggt tacgccagcg
     6961 gtcagtccga ggcctcgggt ctcatcgaca tccgcgccat ggcagcccag catctgggcg
     7021 gcccgatgtc tggcgccaac gtcaacaaag cacccgatct tttcggcggt ggcgatgcag
     7081 cacctgtctt tgcaccggtg gcccccgcgg tcctcgtccc cacggctcct tcagaaggtg
     7141 cgccaaagtg ggtctgggga ttggtcgcag tcggtggcgt ctcgatcatc ggcctgatcg
     7201 tgttcctggt ggtcttcctc tcgggtccga gtacgccgca ggttgcgacg ggtccgacga
     7261 cgggtaccaa tccgaataca ggcacagcga cgggcgccgg cacaaccggc accgcgccaa
     7321 atccgggcac tacggcggcc actgctccga cggaaaacaa gaccaccaag actgaaaccg
     7381 cagccaagac cgaaaccgcg gccaagaccg aaagcggatc gaaggacagc ggttcgagca
     7441 agtcgagcaa gtcgagcaag tcggaaaagg gcgacaaggg cagcaaggca gccgccgcac
     7501 cgaaggaagc cgccgcacca cctgaaaagg ctggcccagg caaagcaccg gaagaggcgg
     7561 cacctccccc gaagaaggca gctgaaaagc cgaaggcggc caaggacgat ctcgactcgc
     7621 tgctcgattc agcctcgagc ggttcgggtg gcaagccgca gaaggcggaa aagaaggatg
     7681 ttcctgaaaa cctcgatcgc ggcacgatca tgagcacgct caagtcagcg aacttcgcgc
     7741 cgtgcaaggc ggaaggcgcg tcgggcgtgg tcaacgtcaa gctgacgatt ggccgcaacg
     7801 gcaaggtgtc ggatgcatcc ggtccgaatg actgcgtcgt caaggtcgta aagggcgtca
     7861 agttccccga gttcagcggc gatccgatga cgctgaccta cccggcactc atccgctaac
     7921 gcgcgctcgg ttttgccgag cagtgttttc cccaaaaagg ctcaggttca ccacctgggc
     7981 cttttttgtt tctaccgacg gaacatctgc gcaccagcaa gaaaaatgca ggtacacgat
     8041 attttcttga ttgggtagtt gactccctga aattgagcgt gctatttacc tgcccgctct
     8101 tgggtttgta gctcaaccgg tagagcagcg gccttttaag ccgttggtcg tgggttcgag
     8161 tcccaccaaa cccacccaag gggaattgca accgcagcga ttgcggtgcg ccgatacagc
     8221 ccgctgccag cagctggctt tggtcggttt gttattgccc ctgcggaaac tctggcgcga
     8281 cgttgtgcgg acgcgctgga cgttccgcgc caggttgcac aatgtggccc tgcgccatga
     8341 gcagcagcac gacgcccgcg acacaacaca gcgcggccca caaaaggccg agcacatgtt
     8401 tgtaaagcgg acgctcacgc agccgcaaag cgagcaggcc aacgcccatc gtaagcagcg
     8461 cgactccgtt gacgatcaaa aaccagcggg gcatggatcg cccgtttagt cccgcacccg
     8521 ccagcgagtg cccgtcggtg tgtccatcac ttcgacgccc agttcccaaa gtgactggcg
     8581 cagcgcatca ctctttgcga aatccttgct gctgcgcgcc tgcgtgcgct cggcaagctg
     8641 cgcttcgatt tgcgccgcat ccaggccgcg gaccgcgatc cgccgccgtc gatgctccag
     8701 aaggtagtcc gctgcgggcc gctcgaggag tccgagcact gcccccgccc ggtccagatc
     8761 ggcagccagt cgaaacagcg tccgccgtcg cacatctttg gccggcggcg gcgtgatttt
     8821 cccatcgagg agccgattga cgagcgaaag cgcaaagctc cattccgcca gcgcgaccgg
     8881 cgtattgaag tcatcgtcga gcgccagata gagccgttcc agccaacctt ccgcttcggg
     8941 cagcacgacc cccggcggct ccggcttcat cccgtgtagc gcgtcttcga gtcgttgctt
     9001 ggtctgatag ccatactcga cgcgcttttc ggcttcttcc agaccgggca gccgaaacgg
     9061 cgccgtttcc gccggatcgt cgggattcat gtcgaggtca aacgcgagcg gattgcgata
     9121 ctgcgtcgtc aaaagccaca gccgcaccgc ctcgccgcca tgccgatcga tgcagttgcg
     9181 ggcgacgaac atgtggtaca gcttgcgcat cgcttcgtcg ctcttggcga tcttgctgcc
     9241 ttccgcgatc agcttttcgc cgtagaccca gcgaaaaccg ataaagccgt tgtgcatcca
     9301 gtagcgcgcg aacgtccctg gaccgtggac gccttgtgac tgggcccgct cgttttcgtg
     9361 gtgcggaaag cgcaggtcga tgccgccgcc gtgaatgtcg aacgtcggtc ccaggtattt
     9421 ttccgacatc gccgagcact cgatgtgcca gccaggacgg cccgcgcccc acggtgatgg
     9481 ccacgaaggt tcgtcgggtt tggccgactt ccacagcgca aaatcagcag catcgcgctt
     9541 gccaggttcg atgtctttgc gcgcaccgca cagcagttct tcccgcggca gccgtgacag
     9601 ttccccgtaa gcagcaaagc tggcgacgga ataatagacg ctaccatcgg attcgtaggc
     9661 gagcccgtgg gcgatcagct tttcgatgtg ggcgatcatc tcgccgatgt gttcggtggc
     9721 gcgtggctcg acatccgacg gctggcagcc cagcgaagcg aagtcgcggt gatattctgc
     9781 cgtccagcgc tcggcgaacg ctttggggtc ttcgccgcac tcgcgggctc tggcgatgat
     9841 cttgtcgtcg acatcggtga tgttgcggac gaacttgacg cgataaccgc gcgcccgcag
     9901 atgccgcgcc acgacgtcaa atgcgacgag tggcctggcg tttccgacgt ggaacaggtt
     9961 gtataccgtc ggcccgcaga catacattcg gatctcaccg gggaggagcg gacaaatttc
    10021 gactttgcgt tgggcaaagg tgtcgtaaat ggacactttg gccaggctcg tcgagccgtt
    10081 gcccatggca gacgaaggct ccgctcctgg ttgatcaccc gagtttccaa atgagtgcat
    10141 agcccaacaa taatcgtcac accgcgaggc ggcaacctct gtcgagatca ttgacgggtc
    10201 atacgaaaaa ccccatactt ccggcggctt ggcggcaggc gtggccgtct tttgctgtgt
    10261 agccggtgcg cggctgcctg tttgccgctt gtccgacgag ccaaaacctc ctgaaaacaa
    10321 cacaagcttg ggtctgattc ctcacagcaa ggtcgctgag atccgcgacc ggacggatat
    10381 tgttgccgtc atcggcgagc acgtacagct gcgtcgagcg ggagtgaact acctcgggct
    10441 gtgtccgttt cacggcgaaa aaacccccag cttcaatgtc agcgcgacga agcagttttt
    10501 ttactgtttt ggctgccaga agtccggcga cgtcatccgt ttcttgatgg aacgagaggg
    10561 gcgcagcttc accgacgtgg ttcgcgatct cgcagcgcgc gcggggatcg acattcccga
    10621 cgacgaagag gaatcgcggc ccgaacgcaa agcgcagcgg cttgcgcaag agagtgagcg
    10681 tctgcggctg ctcaagctca acgcgcttgc ggcgcgctac tacgaggcac agctggcagt
    10741 aaacgagcgt gcgcttgcct acgtcgcaag ccgggggatt ggcgacgaca ttcgcaagag
    10801 cttccgctta ggctacgcgc ccgacagctg ggatggtctt ttgcaagtgc tgcggcagcg
    10861 aggcgtccca cacgagctgg ccgagctggg cggtctgctg attcgtcgcg acggagcgcc
    10921 ggcgctgccc aaaggcgcgc ctccgacgcc gcagacccac tatgatcgct ttcgcgatcg
    10981 gctgctttgt ccgttgctcc tccccgcgca gagcaagcag actgactgcg acgtgattgc
    11041 gtttagcggg cggcttatgc cgacggacac accgcgaccg aatgagggtg caaagtacat
    11101 caactcgccg gagacgccgc tttatcgcaa aggtcaaaac ctgtacggac tgcactgcgc
    11161 acgcgatgtg gttcgtcagc gtcgccaagt gattctcgtc gaagggaact ttgatgtgct
    11221 gtcgctgcac cagcacggct ttggcaatac gctggcgccg atgggcacgg cactgaccga
    11281 aacgcaggtg cggctcttgg gtcgactgct tggcgaagac ggtcatgtgg tgctgatgct
    11341 agacggggac aaggcggggc gagccgcgac gatgaaagac atctcgctgc tgatgctgac
    11401 acaagcccag ctttccgatg tggcgtcgct gtcacagcgc gacattgatg tacgagtcgc
    11461 caagctgccc gacggggaag atcccgatac gctggccaat cgcgatgcgg ctgccctttt
    11521 gcgctgtgtc gccaaagcgc agccggcgat tgactacgtg ctcgatgaag tgctggccgg
    11581 cagcgagtca gacagcctga ccggacgcgc aaagatcatt tcgcgtatga ctccgctttt
    11641 ggcgtcgctg cgcaatgaaa cgctgcggga actctacgtc ggaaaattgg cgatggcgct
    11701 ggcggtcgat gccaagctgc tgtggaaaca gatagaacag gcaaaaacaa gtgggccagc
    11761 gacggtagag ccagcgacta cgccgccgac tgtgcgcagt ttcgacccgc cgcgaaacgc
    11821 aagccagccg cgcagccaag caccgacgca agagagtccg acacgtcgtc cacaaggacg
    11881 cgccgcggcg ggaatgccgt ttgacgggca cctgcgaaat ctgcttgggc ttttggccga
    11941 tcatcctgcg ctgtgggacc agcttggcga cgacacgctg accgcaatac cgaatccggt
    12001 gttgtccgag cttttgcgag acgcgcgcga cctgtgccat agtggcgaga ttgtttccgt
    12061 cgaaaccatc ctcaacatgt gccccgtcga gctgcgtgct ccggtggccg aggcgatgct
    12121 gcaaaatcgg ttcaaagaat cccaacatcc agacgaagaa ctaagccgga tctcgatttc
    12181 gctgcgcgtc caagccatcg ctcgtgaggt cgccgacctc cagcagactt ttcagcgcca
    12241 gagtcgatcc ggtgagcaag aaaacagact tcagcttcta tcgcgcatac aagatctgac
    12301 gaaattacgc gcgtccctct tacaaaatag ccaccccgcc caggcggcag tggctggtac
    12361 tgctcaagga gataccccgc gatgaatcgt gacaacgccg agcgccgcaa gcagcagctc
    12421 attacgatgg gcaaggccaa ggggtacttg acctatgacg aagtaaacga ccacatgccc
    12481 gacgacgtgg tgtcgtccga tcagattgac gactggctgt cgtctctcgg agatgaaggc
    12541 atcgaagtcg tcgactctgc ggccagcgtc aaagtccgct cgaatacggc cccgaaagtt
    12601 cctgaactca tcgaagacga tgagaaaaag gaagaagaag aagaggaaga gttcgacggt
    12661 ggctatagca agaccaacga cccggtgcgc atgtacctgc gcaagatggg ttcggtgtcg
    12721 ctgctcactc gggaaggcga agtcgaaatc gccaaacgga tcgaggaagg cgagcggcgg
    12781 gttctgcaag ttgtgctcaa ttcgtcgatt gccgtcgaag aaatcctcga cctgggcgag
    12841 cggctgaaga agcagaaaat ccgcgtcaaa gacgtcgtcc gcgacttgga tgaagaggac
    12901 gccgatttcg acgaggaatg gcacaccgag cgggtcatca agatcatcga caaggtgcgt
    12961 cggctccagc gtgaaaacga aaagatcctc gaggagctgg agatcccgca gaccaccggg
    13021 ccggcttcgg caacgccgac accaccgccg cctgcgccaa gcaaagccgt cgaggccaag
    13081 cgcaagaagc tgcgcgacaa gatcgaagcc aacaagtccg aaatgttcga gcttctgtcg
    13141 gagctgcgca tcaacaagaa ggtcatcgac cgcatcgtcc agaagctcaa gagcctggtg
    13201 ctgcgcatcg aaaagggcga ggccgagctg gaagagttcg agcgccgcgt cggaatgccg
    13261 gcgaaggaac tggccaagac cctcaaagaa atgaaagcga acgccaccaa ggcgaaagcg
    13321 atcaccaaaa aactcggtgt caccgccgag gaactggaga gcgcagcgct tggcatggcc
    13381 gaagcgaaaa agcggatcaa aaagatcgag gaagaagcca agctttccgt cgacgatctg
    13441 cgcgcgactt acaaagagat ccacgaaggc gagcggatgg cggaaaaggc caaggccgag
    13501 ctggtcgaag cca
//
LOCUS       DAORGN010000139        13414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1918_length_13414_cov_6.180253, whole genome shotgun sequence.
ACCESSION   DAORGN010000139 DAORGN010000000
VERSION     DAORGN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_13414_cov_6.180253"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..87
                     /gene="ffs"
                     /locus_tag="PKL17_14650"
     ncRNA           <1..87
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKL17_14650"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(160..2028)
                     /locus_tag="PKL17_14655"
     CDS             complement(160..2028)
                     /locus_tag="PKL17_14655"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNK46023.1"
                     /translation="MKRVALLLGILATLPQQGCVRSTPKAAPADGGIRVGSLASLILE
                     ELDAELQFSPTQATWLGDHSSDERLDDVRLDAVWRETARLGSMSERATRLAEGLQPAL
                     PVRLDLLSIPDGPEKDTQRLDTLLLLARLEEQRIEREELRPHEKSPLFYAELVAWGLD
                     ELVTSSLATGSGLRALRGRLQAVPALLKEAQRNLKNPPELWVKRAIEVTQQTRDFVAT
                     VLPRLLFGLRGPDPKQSEEVSRLREEAQRALDDYGTFLSRDLLPRSKGEWAQPRDRLM
                     QRMRALELLDVPLETVLAVAEAEHTRTKEQLEELARELSGSQSAGRAISEALREIEED
                     HPRPEELASAVEQSVHRAYELLSDGSLLALPTQRPRIVEMPPYRFGYLQLVTAAPLEP
                     EREPLLQYDPIDANWKDKKQISDHLRMLNRSQILLTVLRDVAPGRLTQQLVVRQKQGS
                     LSSLRVRGHSLALVEGWPLYSAQLAIELLPKGTARDRVQLLLWRHRMVQLGRLIVALR
                     LHQNGSGQPVPSQRIDDAFHFFVDDCYLDEHAARREAERGTYEPLYGLPALGALQLAQ
                     LREDYRAQQGEAFSSSSFHDAVLSAGLVPVTVLRKQLLRTAGQSMRLPAKSETPVK"
     gene            2153..2560
                     /locus_tag="PKL17_14660"
     CDS             2153..2560
                     /locus_tag="PKL17_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46024.1"
                     /translation="MASKPSEPQKGIGAADKAQPTGKAAPVTRATSVEGSSEVEAAKQ
                     AAPVGALSAATAVGKAVSADRVASIADRLRRGVLTPAQAVEELIEDTVRTSLPGLTAS
                     SPLRQKLREILQSYAKDDPYLFAKVRRLSGDQE"
     gene            2590..4059
                     /locus_tag="PKL17_14665"
     CDS             2590..4059
                     /locus_tag="PKL17_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46025.1"
                     /translation="MAVLMLLSSANARKADKKGKKTDVVTATDSAGESGSAKASDAKP
                     DEKTATPAADAAASAGDAAATGSAPAAADAKPAKDKKAKRLRSTTLPLLDKNFPEPVA
                     VALQREMSDALKRNARLDQKELDVRLAEFAQEVPFDQVELARTAYQNGRDAFNKLELD
                     NAIVTLSDAVDQLIAVLPYIKKQELADAMMALAVAQQQRNKNPAMQATLRRLLTWRPT
                     YQVDNDQFPAAINDPLEAARTHQSQQPQGQVKVLSEPPGAQVFVDGEFAGTSPLNVEH
                     LTVGEHYITFKKLGFKRGLRVANIVKDRPAQILGKLDRSEKFLLVEQAISRVGPTMGA
                     KRLDKVVDNLRETLFLDHAIFLKMQRSAPQSSGSEDVTVAAYLYDLRSRSLLSEKTET
                     VKVQGGVPAEGAMALLADKLYEGVDYDSVEVKPDDAPLPVAVKQTPLYKRWWFWTAIG
                     VVVAGGASALAVGLTLRQPSCPDGHTCTGNLVYSLTIPF"
     gene            4108..5880
                     /locus_tag="PKL17_14670"
     CDS             4108..5880
                     /locus_tag="PKL17_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46026.1"
                     /translation="MSHSDKSLSPPTEPADSQRFAAVLAEVRDPTQVDWERAWFGMEP
                     TFTNQKTLKIWQRSSKSEKAELKYFNHRYVLGMEQKVAQAMERKYKRAQRRGESYCLF
                     DRVEREEDVDQWGVKRQNLHFHCHKSGPTFTVRLGLDPEVYEYSIKPVPVAWLYDDRF
                     VEFLQKLVWDVPLRLGLSPTIGQGGCQFSISAKSYLTGSLLCDDIADRLNHPELSCFT
                     MDYPNCDDRCFRATKARRAAFLRIIEQYWAGSFHPRAIGPIRVEHAILDRGFLPAFSP
                     PPDLISREHMSAGPVGTHQEVFQTNFAFARAVRLLAQNIHPGYWQGAHPDSLGYRPDQ
                     IMRYSEGNLNRLRIMGELHVKSDKVLDPHEIPEFDAELAPQHLYHEASWENRAQLSRT
                     SARDFVEALLLDVHRAQYLQKHPVVTPRRTLLQDRLLADAEETLLALGGGDRLAELRT
                     EARKLNLEQSEGRIRSDFIEPETLFWEVYFRLPDAERVQIAREAISGFVGRVSEAAEC
                     DPRHKDAADPMQWHRHRIHPLLWDALALDKGPTSERDIPQREVRKFQAQKEQILLRRP
                     VWSVGKGEAPWDSVSQKLHAVDAD"
     gene            6003..7316
                     /locus_tag="PKL17_14675"
     CDS             6003..7316
                     /locus_tag="PKL17_14675"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK46027.1"
                     /translation="MLRRRIALFADGGFLAHTTRVLEVGRALRTRYGHDVVFCCDGPY
                     SRLLTQAGFPVRPVYSVDREETLRLARRAGACDLRWWQKVAEDSVRSDLEAIDDLTPD
                     LVVGDLRMSLTTSARARGIPLVSVTNGAWTSRFAEPITVPEGHISEKLLGKKLSSALF
                     PVVKRLLIWYWARGFDGVRKRLGLPKLATMYDLIEGDLTLLADLPEYFPIVSSPASFR
                     YIGPIRFREALPRPSWLSSLDPNRPTLYFTMGSSGDARFFSEAVRVFGDTEYQVLITT
                     AGLPSEQFAQHRNIFVEKLADGDSLMAASNMTITHGGNGTIYQALGQGVPVIGIPTMF
                     DQEINLVRVEKLGAGKRLRLRDCQGETLKQTVEHILETPSYLHAAQKLKRRIAQMDGP
                     TNAALHIDHFLTTRDPLSVPREAEQSWAAPTPYLDGEPLLHPIPG"
     gene            7320..8198
                     /locus_tag="PKL17_14680"
     CDS             7320..8198
                     /locus_tag="PKL17_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNK46028.1"
                     /translation="MSTHDQAPRPKTDHIETLSHGERWFLDGFSVIHPPGGVLTQVVD
                     MTRAQDVITRLKAAGVAATYTHLLVRAAALALSRCPDAHKLVCGYKRMTPGQVDIGLS
                     VAGQTNYAPVLVIADAASRPLRNLVDYLKAEVPKTREKEVRDLEGMRKNGWWIPVGFL
                     RRAILRLLRRMFWFQRKLVGTFQLTVLRDVDTVIPILFYSGAALGVGAVRDRVIAVDG
                     QPQVRKTAILSMAFDHRTLDGKRTADLLLQVCQILEGDELLYEADEMPHIAETAAETP
                     LLVAKPSTTPSAYAGG"
     gene            complement(8170..8781)
                     /locus_tag="PKL17_14685"
     CDS             complement(8170..8781)
                     /locus_tag="PKL17_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46029.1"
                     /translation="MAASQTESGTPDSPSDVKDIFALRVTAQAVHLVLSVSGDWESAR
                     ADTVAETERLCDEAERFALSEAFAKRFPGRTGVLRLKTQHPPPEIVRQVLTERGVEME
                     VTSEASTGDKTCELCQRSKLSDQAVSMTDEGWACPCCFRAWLARQDAGRRPRAGTLLG
                     KLSNRLIFPLLLIVVSLFVIGVLYELKRLNQASNVIRQHMPTE"
     gene            complement(8798..10057)
                     /locus_tag="PKL17_14690"
     CDS             complement(8798..10057)
                     /locus_tag="PKL17_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK46030.1"
                     /translation="MLVAAIPFGSAKAAGKLIAVRFGKLVDGSGRVQQSPLVVIEDDR
                     VKSVLPQGAPVPAGVSVLDLSQYTAIPGLIDVHTHMSYLFDDQSGLKPIEQFLKRSSA
                     VTVFLAQRNAQRTLETGVTTVRDLGSFDGMDLSMRELIRRGAMMGPRMLVSGLPLFAT
                     DEAPKPGQPVLPGAADGPAEVMRLARQQVASGVDQIKVVASTGGYDDVTGFQTFSFEE
                     IKAAVDVAHRAGKRCAVHSYGPSGARDAVRAGADSIEHATDIDDATLAEMARRRIYYV
                     PTIDHNRYYADNPKVYGFTSDAVSNLRAFIPKNFETARRAFRMGVPMAMGSDAVFTMF
                     GENTLELGWFVKLGMTPAQALSTATTQAAALLGMEKSVGTVAPGYFADIVAVDGDPLQ
                     DINVVLHKVRWVMKGGAVVVDRATRRP"
     gene            complement(10213..10701)
                     /locus_tag="PKL17_14695"
     CDS             complement(10213..10701)
                     /locus_tag="PKL17_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46031.1"
                     /translation="MSLRVDRWRALAKELTAMEHVHHVAAMPVEKRLERGRAAEQVQA
                     MVMAVLSEAAGAEDSERRVSGLPLVRAAAKYLLTAADRLRRASLLEAWSTHEQKPEQT
                     QAAIQAAREWDDIMRALSTQFGVVHPFAEMVESLALGEREMQTLGVSLQSLHNGEDLL
                     AL"
     gene            complement(10749..11696)
                     /locus_tag="PKL17_14700"
     CDS             complement(10749..11696)
                     /locus_tag="PKL17_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNK46032.1"
                     /translation="MRLAAIDCGTNTAMLLVADLFPDQTPRLCPVADFLEMPRLGQDL
                     DRTGRLHPDAIARTLAAISRQIDRAKELKVDRLVIVGTESLRAAQNSDELLSALAKTG
                     VELRVISGDDEARLAFLSVSESLPLPASGRRSVLDIGGGSTELIVGGQAPERFASVKI
                     GSVRLHERFIFHDPPTPQEIASVTAAIDEGLSKLPQAEGELVALAGTATTVAAIHLGM
                     TSYDGKKVDGLRIPTARLRDVILDLGRRTVAERCQLPGLDPRRADVIFAGALILLRAA
                     ERSNVSDVVVSDRGVRFGVLEELARAAEEKSRPKPLGQG"
     gene            complement(11737..12771)
                     /locus_tag="PKL17_14705"
     CDS             complement(11737..12771)
                     /locus_tag="PKL17_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46033.1"
                     /translation="MTQTAAEKLPYRLWELTFKVSGEVLSSLGWPPFRKFVEPRASRD
                     LSTEMHSLADDIGTAHLALFQSDDPDERDDATFLSDLLQPVRALVLLLYENNLADSER
                     HFGRLRELIASCQLQRELDYPRGIGRWLLLRSLYGLALAACGRADDRTLAQLRDDDEE
                     ARERVLDRIGDRVLTVQGRWRELAAFVHGEHNQLIGKLATPAPKFVEVRLRIELLQDS
                     LRPFGPVSSLYLTPNIPDKKLRNARKFCEVPDEEEVLALLDCTVFGSASDSVLFGNRH
                     LFFRNFIAQNGRPGRIAYHDLVHMAIHRAGDGELMFGDNLISNISGSGLTAADFFSLI
                     RDLQKRLKFL"
     gene            complement(12779..>13414)
                     /locus_tag="PKL17_14710"
     CDS             complement(12779..>13414)
                     /locus_tag="PKL17_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46034.1"
                     /translation="KLEELGRPNEVARELDRLKIDEDATETRMLDGVRVAIDLLRESV
                     GKRKMVVVFSDGIDAAGTKDSYEQVGKKAHDAGVGLATIGYGPFEAGRLRNLIEMSKA
                     AGSTARGSKNAGDIPHHFEQLVDEIVSTHVVRFGLTQSGDGQQHVFQVMFRSGKDDIA
                     SESFTVAQLPQFEPADPAGWPWYYWVLAVAGGLLGLIILLAIIGSLMGPKR"
BASE COUNT         2725 a         4259 c         3947 g         2483 t
ORIGIN      
        1 tgggcgacgg ggatttggtg aaccccgcca ggccaggaat ggagcaacgg tagctattga
       61 accgtgcgct cgcagccacc tcttttgctt cgcttgacgt tgcgctgttt tggttgcttt
      121 cagcgtcgtc cagcctgctt ttgcgaatgc gtgtcccggt tatttgaccg gggtttcgct
      181 cttcgcaggc agccgcatcg actgacctgc tgttcgcagc agttgtttgc gcagcaccgt
      241 cacgggcaca agtcccgccg acagcactgc gtcatggaag ctgctcgacg aaaacgcctc
      301 gccttgctgt gctcgatagt cctcgcggag ctgcgcaagc tggagcgccc cgagcgccgg
      361 caggccatac agcggttcgt aagtgccccg ttcagcctcg cgccgcgccg catgttcgtc
      421 gagatagcag tcgtcgacaa aaaaatggaa ggcgtcgtcg attcgctgac tgggcaccgg
      481 ctgtccgctg ccattctgat gcagccgaag cgcaacgatg agccggccca gctgaaccat
      541 ccgatgccgc cacaatagaa gctgaacgcg atcgcgcgcc gtgcctttgg gcaaaagctc
      601 gattgccagc tgcgccgaat acagcggcca gccttcgacc agcgccaatg aatgtccgcg
      661 cacccgcagc gacgacagcg agccttgttt ttgtcgcacc acaagctgct gggtcaaccg
      721 tcccggcgca acgtcgcgca gcaccgtcag cagaatctgc gagcgattga gcatccgcag
      781 gtggtcgctg atctgctttt tgtctttcca gttggcgtcg atcggatcgt actggagcag
      841 cggctcgcgc tctggttcga gcggtgccgc agtcacgagc tgaagatagc caaaccgata
      901 cggcggcatc tcgacgatgc gcggcctctg ggtcggcaaa gccaacaaac ttccgtcgct
      961 gagcagctca taggcgcgat gtaccgactg ctcgacagcc gacgcaagct cctcgggccg
     1021 tgggtggtct tcctcgattt cgcgcagtgc ttcggaaatc gcccgtccgg cgctctggga
     1081 accgctcagc tcccgcgcga gctcttcgag ctgttctttg gtgcgcgtgt gttccgcttc
     1141 agcgacggca agcaccgtct ccagcggcac atccagcagt tccagcgcac gcatcctttg
     1201 catcagtcga tcccgcggct gcgcccactc gcctttgctg cgtggcaaca gatcgcgcga
     1261 caaaaacgtc ccgtagtcgt cgagcgcccg ctgggcttcc tcacgcagac gcgacacttc
     1321 ttcgctctgc ttgggatcag gtccgcgcag tccgaacaga agccgcggca gcacggtggc
     1381 gacaaaatcg cgggtctgct gtgtgacctc gatcgcgcgc ttgacccaca gctccggtgg
     1441 attcttcagg ttgcgctgtg cttcttttaa aagcgccggt accgcttgca gccgcccgcg
     1501 cagcgcccgc agaccacttc ccgtcgccaa actgctcgtc accagctcgt cgagacccca
     1561 agcgaccagc tcggcataaa aaagcggact tttttcgtgt ggtcgcagct cctcgcgctc
     1621 aattcgctgt tcttcgagcc gggccagaag caaaagcgta tcgagccgct gggtgtcttt
     1681 ttcaggcccg tcaggaatcg acagaagatc gagccgcacc ggcagcgcag gttgcaagcc
     1741 ctccgcaagt cgggtcgctc gttcgctcat gctgcccagc cgagccgttt cgcgccacac
     1801 cgcgtcgagc cgcacatcat cgagccgttc atccgacgag tgatcgccaa gccaggtcgc
     1861 ctgcgtcggc gaaaactgaa gctcggcgtc cagctcttcc aaaatcagcg acgccaggct
     1921 gccaacccga attccgccgt cggcaggtgc ggctttcggc gtcgagcgca cacagccctg
     1981 ctgcggcaaa gtagccaaaa tcccaagcag cagtgcgacg cgcttcatga tccgacaagg
     2041 tacaagcttc gcggcaaatg cccaagttcc caaagaccaa atccgcgctc ggcccacgac
     2101 gaagctgacc ataaaatggc agagttttga tatcctgaga gaaggtcgaa agatggccag
     2161 caaaccgtcc gagccacaga aggggattgg tgcggccgat aaggctcagc ccacgggcaa
     2221 agccgcaccg gttacgcgcg caacaagcgt cgaaggaagc tccgaagtcg aagcggcaaa
     2281 acaggccgcg ccggtcgggg cactttcggc agccacagcc gtgggcaaag cggtatccgc
     2341 cgatcgcgtc gcaagcattg ccgatcgtct gcgccgaggc gtgcttaccc cggcgcaagc
     2401 ggtggaggag ctgatcgagg acacggtgcg caccagcctg ccagggctta ccgcatcgtc
     2461 gccgctccgt cagaagctgc gagagatcct ccagagctat gccaaggacg atccgtatct
     2521 gtttgccaag gttcgacgac tatcggggga tcaggaatga ggcgaggcca ccaatttgtt
     2581 gcggtcggca tggccgtctt gatgcttttg tcgagcgcaa acgcgcgcaa agcagacaag
     2641 aagggcaaaa aaaccgacgt cgttacagcg acggacagcg ccggtgaaag cggcagcgcc
     2701 aaagcgagcg acgccaagcc ggacgaaaaa accgcaacgc cagccgcaga cgccgccgct
     2761 tcagcggggg atgcagcagc gacgggatct gcgccagcag cggcagatgc aaagccggcg
     2821 aaagacaaaa aggccaagcg gctgcgatcg acgacactgc cgcttttgga caaaaacttt
     2881 cctgagccgg tggcggtggc gctccagcgc gagatgtccg acgcgctcaa gcgcaatgcc
     2941 cggctcgatc aaaaggaact ggacgttcga ctcgcggagt ttgcgcagga agtgccgttt
     3001 gaccaagtcg agctggcacg gaccgcgtat caaaacggac gggatgcctt taacaagctg
     3061 gagctggata acgcgatcgt gacgctgtcc gatgccgtcg accagcttat cgcggtgctg
     3121 ccgtacatca aaaagcagga actggccgac gcgatgatgg cgctggcggt tgcgcagcag
     3181 cagcgaaaca aaaacccggc gatgcaagcg acgctgcggc ggcttttgac atggcgaccg
     3241 acatatcagg tcgataatga tcagtttccg gcggcgatca acgacccgct cgaggcagcg
     3301 cgcacgcacc aaagtcagca gccgcaaggt caggtcaaag ttctttctga accgcccggt
     3361 gcgcaggtat ttgtcgatgg tgaatttgcc ggcacgtcac cgctcaacgt cgagcatctg
     3421 acggttggtg aacactacat cacgttcaaa aaactcggct ttaagcgcgg tctgcgcgtc
     3481 gccaacatcg tcaaagatcg accagcgcag attctcggca agctggatcg gtcggaaaag
     3541 ttcctgctcg tcgaacaagc gatttcgcgc gtcggtccga cgatgggtgc caagcggctc
     3601 gacaaagtcg tcgacaacct gcgcgagacg ctgtttttgg accacgcgat ctttctgaag
     3661 atgcaaaggt cggcgccaca gtcgagcggc agcgaagatg tgacggtggc agcgtatcta
     3721 tacgatcttc gctcgcgcag cttgctgtcg gaaaaaaccg agaccgtgaa agtccaaggc
     3781 ggcgttcccg cggaaggtgc gatggcgctg ctcgccgaca aactgtacga aggcgtcgac
     3841 tatgactcgg tcgaagtaaa gcccgacgat gcgccccttc ccgtcgcagt gaagcagacg
     3901 ccgctgtaca agcgctggtg gttctggacg gcgattggtg tcgtcgtcgc tggtggtgcc
     3961 agcgcgctcg ccgtcggact gacgctgcgc cagccaagct gccccgatgg acacacctgc
     4021 accggcaacc tcgtctactc gctgacgatt ccgttttagc ttgcgctaca ctgcgccggc
     4081 acttgctgcc agcgaaggag ccttgcgatg tcgcattccg acaagagctt gtccccgcct
     4141 accgagcctg cggactcgca gcgttttgct gcggtgcttg ccgaagtgcg cgacccgacg
     4201 caggtggatt gggagcgcgc gtggtttggc atggagccga ccttcacaaa ccaaaagacg
     4261 ctcaaaatct ggcagcgctc gtcgaaaagc gaaaaagccg agctgaaata cttcaaccac
     4321 cgctacgtcc tggggatgga acagaaggtg gcccaggcga tggagcgcaa gtacaagcgg
     4381 gcgcagcgtc gcggtgagtc gtactgtctg tttgaccgcg tcgagcgtga agaagatgtc
     4441 gatcagtggg gcgtgaaaag gcagaacctg cactttcact gtcacaagag cggtccgacg
     4501 ttcacggtgc ggcttgggct cgatccggaa gtgtatgaat attcgataaa gccggtgccc
     4561 gtcgcgtggc tttacgacga tcgcttcgtc gagtttttgc aaaagctggt ctgggatgtc
     4621 ccgctgcggc tgggtctatc accgacgatt ggacaaggcg gctgccagtt ttcgatttcg
     4681 gccaaaagct acctgacggg aagcctgctg tgcgacgaca tcgccgatcg cctcaaccat
     4741 ccggagctgt cgtgcttcac gatggattat cccaactgcg acgatcgctg cttccgcgca
     4801 acaaaagccc gacgagccgc ttttttgcgc atcatcgagc agtactgggc aggttccttt
     4861 catccgcggg cgatcgggcc gattcgcgtc gaacatgcga tccttgatcg gggttttttg
     4921 cccgcgtttt caccgccgcc ggatctgatt tcgcgcgaac acatgagcgc cgggcccgtc
     4981 gggacgcacc aagaagtctt tcagaccaac tttgcgtttg cgcgggcggt gcggctgttg
     5041 gcccaaaaca ttcatccggg ctattggcag ggcgcgcacc ccgactcgct gggctatcgg
     5101 cccgatcaga tcatgcgcta cagcgagggg aacctaaacc gcctgcgcat catgggcgag
     5161 ctgcatgtca aaagtgacaa agtgctcgat ccgcacgaaa ttcccgaatt cgacgcggag
     5221 ctggcaccgc agcacctgta tcacgaggcg agctgggaaa atcgtgcgca gcttagccga
     5281 accagtgccc gtgattttgt cgaagcgctg ctgctcgacg tacaccgcgc gcaatatctg
     5341 caaaaacacc ctgtggtgac gccgcgacgg acgcttttgc aagatcgctt gctcgcggat
     5401 gcggaagaaa cactgctggc gctcggtggt ggcgatcgac tggccgagct gcgaaccgaa
     5461 gcgcgaaaat tgaacctcga gcaatcggaa ggccgcatcc gcagcgactt catcgaaccc
     5521 gagacgctgt tttgggaagt ctatttccgc ttgcccgacg cagaacgggt gcagatcgcc
     5581 cgcgaagcga tctctggttt tgtgggccgg gtgtccgaag cagccgagtg cgatccgcgc
     5641 cacaaagacg cagcagaccc catgcagtgg catcggcatc gcatccatcc gctgctctgg
     5701 gatgcgctgg cgctggacaa aggtccgacg agtgaacgag acattcccca gcgcgaagtg
     5761 cgaaaattcc aggcccaaaa ggaacaaatc cttctgcgca ggccggtgtg gtccgtcgga
     5821 aaaggcgaag cgccatggga ctctgtttcg caaaaactcc acgcggtcga tgcagactga
     5881 gggggacagt cccacccatt tctccgacgg caaagtgcat tttctgatct tttcggggcg
     5941 acaaaaaagg tgttagaagc caaggacgtt ccggtcgttt tcggaatgga aggaagggct
     6001 tcatgttacg tcgccgcatc gcactgtttg cagacggggg atttttggct cacaccactc
     6061 gcgtgctcga agtgggcaga gcgcttcgca cccgctacgg ccacgatgtg gtcttctgct
     6121 gcgacggacc gtactcgcgg ctgctcaccc aagcgggctt cccggtgcgt ccggtctatt
     6181 cggtcgatcg cgaagaaacg ctgcggctgg ccaggcgcgc gggtgcctgc gatctgcgct
     6241 ggtggcaaaa agtcgccgaa gactcggtgc gatcggacct cgaggccatc gacgatctga
     6301 cgccggatct ggtggtcggt gatctgcgaa tgtcgctgac gacgagcgcg cgcgcccgtg
     6361 ggattccgct cgtgtcggtg acaaacggcg cgtggaccag ccgctttgcc gaaccgatca
     6421 cggttcccga aggccacatt tcggaaaaac tgctcggcaa gaagctttcg tcggcgctgt
     6481 ttcccgtcgt caagcggctg ctgatttggt actgggcgcg cggttttgac ggcgtgcgca
     6541 agcggctcgg actgcccaag ctggcgacga tgtatgacct catcgaaggg gatctgacgc
     6601 tgcttgccga cctgccggaa tatttcccga tcgtgtcgtc gccagcgtcg ttccgctaca
     6661 tcggaccgat tcgctttcgc gaagccctgc cgcgtccaag ctggctgtcg tcgctcgatc
     6721 cgaatcgccc gacgctgtac tttacgatgg gctcgtcggg ggatgcacgc ttctttagcg
     6781 aagcggtgcg ggtgttcggc gacaccgagt accaagttct gatcacgacg gcgggtctgc
     6841 caagcgagca gtttgcccag caccgcaaca tctttgtcga aaagctcgcc gacggtgatt
     6901 cgctgatggc ggcgtcgaac atgacgatca cgcacggcgg caacggcacg atttatcaag
     6961 cgctcgggca aggcgtgccc gtgatcggca ttccgacgat gtttgaccag gaaatcaacc
     7021 tggtgcgcgt cgaaaagctc ggggcgggca agcggctgcg gctgcgcgac tgccagggcg
     7081 aaacgctaaa acagactgtc gagcacatcc tcgaaacgcc gtcgtatctg catgcggcgc
     7141 agaagctcaa acggcggatt gcccagatgg atggaccgac gaacgccgcg ctgcacatcg
     7201 atcacttcct gaccacgcgc gatccgcttt ccgtcccgcg cgaagctgaa cagagctggg
     7261 ccgcaccgac gccatacctc gacggagaac cgctgctcca tccgattccg ggctagccca
     7321 tgtcgacgca cgaccaagcg ccaaggccca aaaccgacca catcgaaacg ctgtcgcacg
     7381 gagagcgctg gtttctggac ggtttttccg tcattcatcc ccccggcggc gtgctgaccc
     7441 aagtggtcga catgacccgc gcgcaagatg tcatcacgcg actgaaagca gcgggcgtcg
     7501 cggcgacgta tacgcatctg ctggttcggg cggcggcgct cgcactttcg cgctgtcccg
     7561 acgcgcacaa gctggtctgc ggttacaagc ggatgacgcc agggcaagtc gacatcggct
     7621 tgtcggtggc gggacagaca aactatgcgc cggtgctggt gattgccgat gcagcgtcgc
     7681 gacccctgcg caacttggtc gattatctga aagcggaagt gcccaaaacg cgcgaaaaag
     7741 aagtccgcga cctcgaaggg atgcgcaaaa acggctggtg gattccggta ggttttttgc
     7801 gtcgcgcgat cttgcgtctg ctgcgtcgga tgttttggtt tcagcgcaag ctcgtcggga
     7861 cattccagct tacggtgctg cgcgatgttg acacggtgat cccgattctg ttttattcgg
     7921 gcgcggcgct cggcgtgggt gcggttcgcg atcgagtgat tgcggtggat ggccagccgc
     7981 aggtgcgcaa gacggccata ttgtcgatgg catttgatca ccgaacgctc gacgggaaac
     8041 ggacggcaga cttgctcttg caggtctgtc agatcctcga aggggacgag ctgctatatg
     8101 aagcagatga aatgcctcat attgccgaaa cggcagcaga aacgccgctg ctcgtcgcca
     8161 agccgtcaac tactccgtcg gcatatgctg gcggatgaca ttgctggctt ggtttagccg
     8221 ctttagctca tacagcacgc cgatcacaaa cagcgacacc acgatcagca gcagcgggaa
     8281 aatcaaccga ttggacagct tgccaagcag cgtcccagcg cgcggacgac gcccagcatc
     8341 ttggcgtgca agccacgcgc gaaaacaaca cggacaggcc cagccttcgt cggtcatcga
     8401 aacagcttgg tcgctcagct tggatcgctg acacagttcg caggtcttgt cgcctgtgct
     8461 ggcttcactt gtgacttcca tctcgacgcc gcgctcggtc aagacctgcc gaacaatctc
     8521 cggcggcgga tgctgcgtct tgagccgcag cacacccgtc cgacccggaa atcgcttcgc
     8581 aaacgcttcg cttagcgcaa aacgctcggc ctcatcacag agccgctccg tttcagcaac
     8641 ggtatcggcg cgcgccgatt cccaatcgcc cgagaccgac agcaccaaat gaaccgcctg
     8701 tgccgtcacc cgcagcgcaa aaatgtcctt cacgtccgag ggagaatcag gggtgccaga
     8761 ctccgtttgt gaagcagcca tcatgtccct tttgcgttta aggccgccgc gttgctcggt
     8821 cgacgaccac cgccccaccc ttcatgaccc agcggacttt gtgcagcacc acgttgatgt
     8881 cttgcagcgg gtctccgtcg accgcgacga tgtccgcaaa gtatccgggc gccaccgtgc
     8941 cgaccgattt ttccattccc aaaagcgctg cggcttgggt ggttgccgtc gacaacgcct
     9001 gcgccggagt catccccagc ttgacgaacc agcccagctc caaggtgttt tcaccaaaca
     9061 tcgtgaacac cgcgtcgctg cccatcgcca tcggcacacc catccgaaac gcccgccgtg
     9121 ccgtctcgaa gtttttggga atgaacgccc gcaggttgct caccgcgtcg ctggtgaagc
     9181 catacacttt gggattgtcc gcgtaatagc ggttgtggtc gatggttgga acatagtaga
     9241 tgcggcgacg agccatctcg gccagcgtcg catcgtcgat gtccgtcgcg tgctcgatcg
     9301 aatcggcacc ggcacgcacc gcatcgcgcg cacccgacgg accataggaa tgaacggcgc
     9361 agcgcttgcc agcccggtgg gcgacatcga ctgcggcttt gatttcctcg aacgaaaacg
     9421 tctgaaagcc ggtcacatcg tcataaccgc ccgtcgacgc caccactttg atctgatcga
     9481 ccccggacgc aacctgctgc cgtgcaagtc gcatcacttc ggcgggacca tcggcggcac
     9541 caggaagcac cggctgtccg ggcttggggg cttcgtccgt cgcaaacagc ggcagaccag
     9601 acaccagcat gcgcggaccc atcatcgcgc cgcgccgaat cagctcgcgc atcgacagat
     9661 ccattccgtc aaacgaaccg aggtcgcgca ccgtcgtcac gccggtctcc agcgtgcgct
     9721 gcgcgttgcg ctgggccaaa aacaccgtca ccgcagacga acgcttgaga aactgctcga
     9781 tcggctttag tcccgactga tcgtcgaaca gatagctcat gtgggtgtgg acgtcgatga
     9841 gcccaggaat cgccgtgtac tggctcaggt ccagcaccga aacgcccgcg ggaaccggcg
     9901 ccccctgcgg cagcacgctt tttacccgat cgtcttcaat gaccaccagc ggactttgct
     9961 gcacacgccc cgaaccgtcg accagcttcc caaatcgcac tgcgatgagc ttgcccgccg
    10021 ctttcgcact gccaaaaggg atcgcggcca ccagcatggc gccaaacgct gcgcaccaaa
    10081 tggcttgccg gcgaactccg atacggacgg aattcgtgct cgcagtcata cgctgttgtc
    10141 tccaggttcc agctcggttc atacagaata ggcgtgaacc caaggcggtg tctggatctt
    10201 tctgcgcgac ggttacagcg cgagaagatc ctcaccattg tgaagcgact gaagcgacac
    10261 accgagcgtc tgcatctcgc gttcaccgag cgccagcgat tcgaccattt cagcgaacgg
    10321 atgaaccacg ccaaactgcg tcgacagcgc ccgcatgatg tcgtcccact cgcgcgccgc
    10381 ttggatcgcc gcttgcgtct gttccggctt ctgctcgtgc gtgctccacg cttccaaaag
    10441 cgacgcacgc cgcagccgat ccgccgccgt caaaagatac ttggcagcgg cccgcacaag
    10501 cggcagacca cttacgcgac gttcgctgtc ttcggcgccc gcggcttcgg acagaaccgc
    10561 catcaccatc gcctgcactt gctcggcggc ccgtccccgc tcgagccgct tttccaccgg
    10621 catcgcagcg acgtgatgca catgctccat cgctgtcagc tctttggcca acgcacgcca
    10681 gcgatctacg cgaagagaca acgaaaaacg attcgataac ggactcgaag tgctcacaaa
    10741 tccgaatgtt aaccctgccc aagcggcttc ggcctacttt tttcctcggc tgcgcgggca
    10801 agctcctcaa gcacgccaaa gcgcacgccg cgatcgctca ccaccacgtc ggaaacgttc
    10861 gaccgctcgg cggctcgcaa caaaatcagc gccccagcaa aaatcacatc ggcacgtcgt
    10921 ggatcgagtc ccggaagctg gcatcgctca gcgacagtgc gacggcctag atccaagatc
    10981 acgtcacgca gcctggcggt gggaatgcgc agaccatcga cctttttgcc gtcgtagctt
    11041 gtcattccca agtggatcgc cgccaccgtg gtggccgtcc ccgccagcgc caccagctcg
    11101 ccttcggctt gcggcagctt cgacagccct tcatcaatcg ccgccgtcac cgacgcaatc
    11161 tcttgcggcg tcggcggatc gtgaaaaatg aagcgctcgt gcagtcgcac cgatccgatc
    11221 ttcacgctcg cgaagcgttc gggcgcttgc cccccgacga tcagctcggt tgatccgccg
    11281 ccgatgtcga gcacgctgcg ccgaccgctc gccggcagtg gcagactttc cgacaccgac
    11341 agaaacgcca gccgcgcttc gtcatcaccc gaaatcaccc gcagctcgac gccggttttc
    11401 gccagcgccg atagcagttc gtcgctgttt tgcgccgcgc gcagcgattc ggttcccacg
    11461 atgaccaagc gatcgacctt cagctctttg gcgcgatcga tctgccgaga aatcgccgcc
    11521 agcgtccgcg caatcgcatc gggatgcagc cgcccggtgc gatcgaggtc ttgcccaaga
    11581 cgcggcattt ccaaaaaatc agcgacggga caaagccgag gtgtctgatc gggaaacaga
    11641 tcggccacca gcagcatcgc ggtattggtt ccacagtcga tcgcagcaag acgcatcgcc
    11701 gccacgctag cagatccgat cgacttgggc gctgcgtcag aggaacttga gccgtttttg
    11761 cagatcgcga atcagcgaaa aaaagtccgc tgccgtcagg cccgaccccg aaatgttgga
    11821 aatcaggttg tcgccaaaca tcagctcgcc gtcgccggcc cgatgaatcg ccatgtgcac
    11881 aaggtcgtga taggcgatcc gtccggggcg accgttttgc gcgatgaagt tgcggaaaaa
    11941 caggtgccga ttgccaaaca gcaccgagtc agacgccgat ccaaacaccg tgcagtcgag
    12001 cagcgccagc acttcttctt cgtcgggcac ttcgcagaac ttgcgcgcgt tgcgcagctt
    12061 tttgtcggga atgttcggtg tcaaatacag cgatgacacg ggcccaaacg gtcgcagcga
    12121 atcttgcagc agctcgatgc gcagccgcac ttcgacaaac ttgggtgccg gcgtcgccag
    12181 cttgccaatc agttggttgt gttcgccgtg gacaaaagcc gccagctcgc gccacctgcc
    12241 ctgcaccgtc agcacccgat cgccgatgcg atccagcacc cgctcgcgcg cttcttcgtc
    12301 gtcgtcgcgt aactgggcca gcgtccgatc atcggcccgt ccacacgccg ccagcgccag
    12361 accatagagc gatcgcaaca agagccagcg cccgatgcct cgcggatagt ccagctcgcg
    12421 ctgaagctga cacgacgcaa tcagctcgcg cagacgacca aagtgccgct cgctgtccgc
    12481 caggttgttt tcgtacagaa gcagcaccag cgcacgcacc ggttgcaaaa gatcggacaa
    12541 aaacgtcgca tcgtcgcgct cgtcgggatc gtcactttgg aacagcgcca ggtgggccgt
    12601 cccaatgtca tccgcgagcg agtgcatttc cgtcgacaga tcgcgacttg cgcgcggctc
    12661 gacaaacttg cgaaacggcg gccagccaag cgacgacaac acctcgcctg acactttgaa
    12721 tgtcagctcc cacagccgat acgggagctt ttcagcagcg gtctgggtca tggccgagct
    12781 accgcttcgg acccatgagc gacccgatga tcgccagcaa aatgataagc ccgagcaggc
    12841 cgccagcgac ggcaagaacc caataatacc agggccagcc agccggatct gccggttcaa
    12901 actgcggcag ttgggcgacg gtgaacgact cggacgcgat gtcgtctttg ccggaccgga
    12961 acatgacttg gaaaacgtgc tgctgtccgt cgcccgattg ggtcagacca aaccgcacca
    13021 catgcgtcga cacgatctca tcgacgagct gctcgaagtg atgcggaatg tctccggcgt
    13081 ttttgctgcc tcgcgccgtc gagcccgccg ctttcgacat ctcgatcagg ttgcgcagcc
    13141 gtcccgcttc gaacggtccg tagccaatcg tcgccaagcc gacgccggca tcgtgcgctt
    13201 tttttccgac ctgctcatag ctgtctttgg ttccagccgc gtcaattccg tcggaaaaca
    13261 ccacgaccat tttgcgcttg ccgaccgatt cgcgcagaag gtcaattgcc acccgaacgc
    13321 cgtcgagcat ccgggtctct gtcgcatctt cgtcgatttt gagccggtcc agctcgcgcg
    13381 cgacttcgtt tggccgtccc agctcttcca gttt
//
LOCUS       DAORGN010000140        13258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1946_length_13258_cov_5.967432, whole genome shotgun sequence.
ACCESSION   DAORGN010000140 DAORGN010000000
VERSION     DAORGN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_13258_cov_5.967432"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..2018
                     /locus_tag="PKL17_14715"
     CDS             21..2018
                     /locus_tag="PKL17_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46035.1"
                     /translation="MSVTYLLMGCNAEPNLKDSLDNKSQSASGWNGLGWNGLGWNGLG
                     WNGLGWNGLGWNGLGWNGLGWNGLGWNGLGFTGIVPDWNLSGWLNEAGISAAERNSRF
                     QGLSYWTSCACGSTDMLTWNGTNPFVPGATETRYFPGELDLAPTWCRGTGTVPLEEMQ
                     AVSACLFARVNSKGIHNALSLRGVNSSLALGDNEKLFMAYPEGQFFGNIWDASDKIFD
                     PTTSRYYKNLRAYSCIFPSGASSASLNRDLQIIVGRDCEWGNCSSHMKTLLQCSQSGS
                     TDGSQSVAYIDPTLTMSLGSDSDPSVSYSGANASYANGYQDTITGSQFVEYRGNKYRQ
                     ISVFLGAWADLETENWKDASYKCDDGCAPMDGCKCYDTYCRGGEQAQGEAVRCEAPSA
                     CNNCQTVVRSPGPKCGLSQACKGPDDCVRDRKLVALDAACWLPIQFTRPWDILSGSWV
                     SGTANPHKAGTLLFRYSNGTPEELSLQIRDQIRNLPIPGTGFQPTGSWDNYDNKYIYP
                     IYPGRTTFSPGTPGIEIAIAGDPASQFPHLDYAQIFIGPPLGVSPRELYWNTEPVDID
                     PNGVVCTKVRVEKDSDFRLASLVRVNLKLRDADPAGLDVTLSHNGQKAAYLPSKDDAS
                     WSEWDETFAKDDPREDAWEVCVNGNGSSGRIEAVQLELSKP"
     gene            complement(2044..5442)
                     /locus_tag="PKL17_14720"
     CDS             complement(2044..5442)
                     /locus_tag="PKL17_14720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46036.1"
                     /translation="MAEDRRSPPASHVASSDPESEPATIDMVPGGHVSQDALPPDDGD
                     EPTTDLPATQSVSAVAGSQSASLGAKAPRSVPSMPKVGIPRSLQKWDRYQVIKVLGAG
                     GMGAVYMARDPRLNRLVALKLVHPGLGHAGGSTGAVFVKRFEREARLQASLDHPHICK
                     VYEIGEMPSDTDDSGYPYIAMQLISGKPLHLAQLEMSLFEKVQVLRKVADAMHAAHRQ
                     GLIHRDIKPSNIMVERTADGNWHPYVMDFGLARETTGSDNSRSGIIEGTPRFMAPEQA
                     RGDTRHLDRRTDVYALGVTLYEILLGRSPYDGTVDIDILLAVLTVDPKPMRAVDPTIP
                     PDLEAITLKCLEKEQPARYDSAKALADDLQRFIDGEPVLARRIGLTQRLYRQARRHKP
                     LVALGSALLLSLVGLIWYGVRTRLATIEREEKAQKQAQLAQRLGQEISKMEWLLRSAR
                     QLPLHDLEREKAIIRKRMQQLQTELSGYGSSGRGLAHYALGRGHLALHEYPQALSELR
                     QAQAEGNDSADVHYALGLLLGKHYEQAMYEARLAGGGEWADKQLKELEPKFLQPALLS
                     LQKSRGLLSDTPGYLEALIAYYQRDFAKAEQLAAAAVQASPWLYEGLKLQGDMQLQRA
                     LKARDSGQDEEAKKAFSAAVRHYQDAAQIGQSDAEVYEGLAEAWVRQVEMAATRGQPT
                     EVAYAAAIENSDKITTAEPHSTAGPLKKAYAALLTMAVTGSGTSSMERVQQCLVASQA
                     VLDRDPSHPYARDVAAGCNAFAADGAQSRGEDPVPKLQKALALLEPAVRQYPHFLWGI
                     NDLSNVYVTTGNYLQQRGMPGAREHFDKALTYAQMAIRLDSSYLIAIQNKLYINARIV
                     EELTSAQSIHQLLDETDRVLAQCVEKNAQFQQCHINYLSVYARASARLMLAGHDVQAK
                     IQRGKETLATARKLGGAFLDLEQYAALLLLVEARDRMAHKQDPSSSLTALQETLAKCL
                     ALSPQDAMCRTILAQAEWVKADFATQQKAQAMPLLQAARAKATLATQSPETYPDAWQV
                     LAESQLRLVGSDETTPKVQLRALQDGLAATDKVFALNPNHALGHATRGQLLLLWAQKD
                     KDALQKRDRAIAATKEMERAIRQDALLTVRMLSLLPVARALTSS"
     gene            complement(5472..6746)
                     /locus_tag="PKL17_14725"
     CDS             complement(5472..6746)
                     /locus_tag="PKL17_14725"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNK46037.1"
                     /translation="MLIPLLSVAALLCAIVLSMWSRLNVGLLALAFAYGLGVFGAQMS
                     IRQVMAGFPVPLFLTVLSLTLLFGHAQQNGTLGRVAGYAVQLARGRPGLLPPIFFLLA
                     LSLGTVGAGNIGAVAMLAPVAMAVAHEAGIPSFLMALLVACGGNAASLSPLAPTGVIA
                     SDLMGRIGLFGYGWSNYAQCLLAHTTVAMAGYLLFGGGKLLFTEPTPQQRERLDELLL
                     SQQKPLQTKHAVTLFLLFAVLLTVSIAKIEIGAVALAAAVLLSVLRVSDEEQVLRELP
                     WGTMLMVCGMTCLMGILERTGGIDLFTWLIEKLSTQRRAPFVIGFCTGLLSAYSSSSG
                     VVLPAFLPILPKLIQMLGGGDLVALANSVNVGAHLVDVSPLSTLGALCLANAQGDDGK
                     LYRQLLAWGLSMAVVGALYCQLVFGVLRLGSH"
     gene            complement(6784..8022)
                     /locus_tag="PKL17_14730"
     CDS             complement(6784..8022)
                     /locus_tag="PKL17_14730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNK46038.1"
                     /translation="MPVAKVVLKRGRANPVWRGHPWVFSGAIQKLPPGLSPGDIVAVH
                     DQDDKLIGHGFANPRSQIAVRMLSLGELHAGALPGVADHTLPPTALVASLISRRIHEA
                     SLLRRSIGLPRSDTTAYRLVNSEGDGLPGLIVDIFGDVAAVQFSALGMKLCEEAVFAA
                     LRSLPNPPAVIVEAAAGSFAKIEGFSSQQRVVFGDESLLAGGTLCLENGIRLRVDVRD
                     GQKTGAFLDQRDNRLLLGKFCAGAKVLDVYSYAGGFALQALRHGAVEATCVDISPKAL
                     KLCSENAELNSLGLLHTVESDAFRFLEGATPLSFDVTVVDPPKFARAQKDLPAALKGY
                     QRLNALAMGTVRRGGLLATCSCSQLVDEQSFLRMLAAAALDAGRRVTVLYRGSQGLDH
                     PVPPSFDEGRYLKFVLLGVL"
     gene            complement(8047..9078)
                     /locus_tag="PKL17_14735"
     CDS             complement(8047..9078)
                     /locus_tag="PKL17_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNK46039.1"
                     /translation="MRTYLSSGDVFLGHKLVGEHPERPERLQAILTAIDTSDLVGRFR
                     NIPPRRATQDELLRVHSASYLNDLRQRMGDLQPKGQGWIDPDTYYGPGSYEAALHAAG
                     TATDLALRIAQSKGEPGDNGFALVRPPGHHASADRAMGFCLLNNIAVAAAAVRATGRR
                     VAIVDFDVHHGNGTEEIFCRDGGVLFVSLHQFPFFPGSGAATSIGEGNGRGCIVNIPL
                     PEGSAGPEYKLAFKRVVLPVLRRFAPDVILVSAGFDGHSRDTLAGMSLVEDDYAQMVS
                     SLLGVQSRMLLVLEGGYHQSALSASVLSVLRVLIDPSGYRPSGHDAGTAPSDVVRQQL
                     IEIQKLHGL"
     gene            9165..11225
                     /locus_tag="PKL17_14740"
     CDS             9165..11225
                     /locus_tag="PKL17_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNK46040.1"
                     /translation="MSPRRSTEHLATAHGSPEDAHGPAAKSASSSTSQQPYVPYIAPD
                     ADVAELTPKAVLLGALFGILFGAATVYLALRAGLTISASIPISVLSIAVFKKLGKSTI
                     LENNIVQTLGSAGESIAAGVVFTVPALLFLAQGDSFFRYGQILTLAAVGGVLGILFMI
                     PLRRSLIVKEHGKLPYPEGTACAEVLMVGERGGDLARRVFHGVFAAAGYWLLMGVLKL
                     WRDTPGLRTGPKSVYPNANLECNITPEYLGVGYIVGPRIAAEMTSGGVLSWLALIPLI
                     SMYVPDAQRITDLHALGYNDAWITSHSVAEQVYRAYVRYIGAGAVACAGLMTLLKTLP
                     TIVSSFRDSIRDLRGGQKNETIRTERDLPLGLVVVGTAVLGLLIALLPNLPGKFPGSL
                     VISALIVLFGFFFVTVASRIVGIIGSSSNPISGMTIATLMGTCLVFITIGWRGDAYQA
                     AALLVGSVVCIASANAGATSQDLKTGFLVGATPYKQQIGLMIGVVVSVLVIGGTLLLL
                     DRSIPGVQHAIGTEKFPAPQSMLMATMIKGQLSQQLPWGPVLVGVFMALMAQLAGAHA
                     LSWAVGAYLPLSTTSPIFVGGMVRWLVNRRRTLLSGGKAAAAESDIGPGMLYATGLVA
                     GGSLAGMLIAFLQGFAEPVANKLDLGRDYWTRLGLGGDVIAAGAFFVLSVFLIRQALS
                     SDKK"
     gene            complement(11328..11756)
                     /locus_tag="PKL17_14745"
     CDS             complement(11328..11756)
                     /locus_tag="PKL17_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNK46041.1"
                     /translation="MLRPGSIAQELTITHVVGEQDLAPHVAATGTGGGPEHNLFPLVL
                     GTAKLIAWMELAAAKAMEPMLLPGELTAGIVVRVEHHAPTPLGGTVRMTARYLGLEGK
                     FHRFEVVAHDDAGEIFRGQHVRAVVEARRLEKGASRRLRR"
     gene            complement(11763..12386)
                     /locus_tag="PKL17_14750"
     CDS             complement(11763..12386)
                     /locus_tag="PKL17_14750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNK46042.1"
                     /translation="MSSHHPPAHSETTPQTSGKVGKVGVRVVGRPDSLEQRVYLPEVI
                     RGLGVTAKHFFANLFSRKYTVTVEYPEQKLKYADRHRGLHRLMLRDDGAVRCVACMLC
                     PTVCPAHCITIVPEETGNKNEKRPRIFEIDELRCVVCGLCVEACPCDAIRMDSGVHAP
                     AVDFRGDAIQTKIDMLKRGTQSVATQGGVGSDWRQKQPSVKQYDPPK"
     gene            complement(12390..>13258)
                     /locus_tag="PKL17_14755"
     CDS             complement(12390..>13258)
                     /locus_tag="PKL17_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNK46043.1"
                     /translation="RFLFALAPVMAIAPVLITFAVIPFGPDLCVGQVLSVVSDPSQCK
                     ATIPLQVARMDVGMLFYFALASLAVYGTTLAGWASYNKWSLLGGLRASSQMMSYEVCM
                     GLSLMGAFLVYGSLEPNAIVLSQGSNILNWGIVTQPLAFVLFLTAAIAETKRAPFDLP
                     EGEPELIGYFVEYSGMRFGIFFLAEFIETIFLSAVVTTVFLGGWQVPGLDSSGFGSLH
                     LPHLAVVLIQVVAWSLKILFLCWLQLLIRWTLPRFRPDQLMRLGWKQLLPLSLLNIII
                     TAAVVLWRSGGK"
BASE COUNT         2647 a         4219 c         3886 g         2506 t
ORIGIN      
        1 gaaaacacgt actcgctctg atgtccgtta cctacctatt gatgggctgc aatgcagagc
       61 caaacctaaa agacagctta gacaataagt cgcagtcggc ttcgggctgg aacggccttg
      121 gctggaatgg cctgggctgg aatggcctgg gctggaacgg ccttggctgg aacggccttg
      181 gctggaacgg ccttggttgg aatggccttg gctggaatgg tcttggctgg aatggccttg
      241 gcttcactgg catcgtgccg gactggaatc tcagtggatg gttaaacgag gctggcatct
      301 ctgccgccga aagaaactcc cgcttccaag gcctttcgta ctggaccagt tgcgcctgtg
      361 ggtcgaccga tatgctcact tggaatggca ccaatccgtt tgttccgggt gccaccgaaa
      421 cacgatattt cccgggtgag ttggatctgg cgccgacttg gtgccgtggt acgggaaccg
      481 tcccgctcga agaaatgcag gcggtttcgg cctgcttgtt tgcacgagta aactccaagg
      541 gaatccacaa cgcgctgtcg ctgcgtggcg tgaactcgtc gctggcactc ggggacaatg
      601 aaaagctctt tatggcgtat ccagagggtc agttcttcgg caacatctgg gacgcgagcg
      661 acaagatttt cgatccgacg acgagccgct actacaaaaa cctgcgcgct tattcgtgca
      721 ttttcccgtc gggcgccagc agtgcatccc tgaaccgcga tctgcaaatc atcgtgggcc
      781 gcgactgtga gtggggaaac tgctcgtcgc acatgaagac gctgcttcag tgttctcaaa
      841 gcggcagcac cgatggttca cagtcggtgg cctatatcga tccgacgcta acgatgtctt
      901 tgggaagcga ttccgatccg tccgtcagct acagcggcgc aaacgcaagc tatgccaacg
      961 gctaccaaga cacgatcacc ggttcgcagt ttgtcgaata tcgcggcaac aaataccgcc
     1021 aaatttccgt gttcctcggc gcttgggccg acctcgagac ggaaaactgg aaagacgcca
     1081 gctacaagtg tgacgatggc tgcgcgccga tggacggctg caagtgctac gacacgtatt
     1141 gccgtggcgg cgaacaggcc cagggcgaag ccgtccgctg cgaagcaccg agcgcctgca
     1201 acaactgcca gaccgtcgtt cgctcgcctg gtcccaagtg cggcctgtcg caagcctgca
     1261 aaggccccga cgactgcgtg cgtgaccgca agctggtggc actcgatgcg gcgtgctggc
     1321 tgccgatcca gtttacgcgc ccgtgggaca tcctgagcgg ctcgtgggtc agcggaacgg
     1381 ccaatccaca caaagccggc acgctgctgt tccgctattc aaacggcacg cccgaggaac
     1441 tcagcctcca gatccgcgac cagattcgca acctgccgat ccccggcacc ggcttccaac
     1501 cgacgggaag ctgggacaat tacgacaaca agtacatcta tcccatctat ccggggcgga
     1561 cgacgttttc tcctggaacg ccaggaatcg aaatcgccat cgccggcgat ccagcgtcgc
     1621 agttcccgca ccttgattat gcgcaaatct tcatcggacc gccgctcggc gtctcaccgc
     1681 gcgagctgta ctggaatacc gagcccgtcg acatcgatcc aaacggcgtc gtgtgcacca
     1741 aagtgcgcgt cgaaaaagac agcgacttcc gactggccag cctggttcgc gtaaacctca
     1801 agctgcgcga tgccgatcca gccggtctcg acgtgaccct ttcacacaat ggccaaaagg
     1861 cggcctatct gccgtccaaa gacgacgcaa gctggtccga gtgggatgaa acgttcgcaa
     1921 aggacgaccc acgagaagac gcctgggaag tctgtgtaaa cggcaacggc tcctcgggtc
     1981 gcatcgaagc cgtgcagctc gagctgagca aaccgtagcg ccatcccccg caccgcgcgg
     2041 acgctaggac gaagtgagcg cccgcgccac cggaagcaaa ctcaacatcc gcaccgtgag
     2101 cagcgcatct tgccggatcg ctcgctccat ttctttggtc gcggcgatgg cacgatcgcg
     2161 tttttgtagc gcgtctttgt ccttttgcgc ccacaacagc aaaagctgtc cgcgcgtcgc
     2221 gtggccgagc gcgtgattgg ggtttagcgc aaacaccttg tccgtcgctg ccaagccgtc
     2281 ttgtaaggcg cgcagctgca ctttgggcgt ggtttcgtcg ctgcccacaa gccgcagctg
     2341 ggactccgcc agcacctgcc acgcatcggg atacgtctct ggactctgcg tcgccagggt
     2401 cgctttggca cgcgcagcct gaagcagtgg catcgcctgc gccttctgct gcgtcgcaaa
     2461 gtccgccttc acccattccg cctgcgccaa aatcgtccgg cacatcgcat cttgcggact
     2521 gagcgccaga cactttgcca gcgtttcttg caacgccgtc agcgacgaag atggatcttg
     2581 cttatgcgcc attcgatcgc gcgcctcgac cagcaagagc agcgccgcat actgttccag
     2641 atccaaaaac gcgccaccga gcttgcgcgc cgtcgccagc gtttctttgc cgcgctggat
     2701 tttggcctgc acatcgtggc ccgccagcat caaccgtgcc gaggcccgcg cataaacact
     2761 caaatagtta atatggcact gctgaaactg agcatttttt tcgacacact gagccagcac
     2821 cctatctgtt tcatctaaca attgatgtat cgactgtgcg cttgtcagtt cttccacaat
     2881 tcttgcgttg atatataatt tattttgaat cgcaatcagg taactactat ccagccgaat
     2941 tgccatctgt gcatatgtca gcgctttatc aaaatgttcc cgtgcacctg gcatcccacg
     3001 ctgctgcaga tagtttcctg tggtcacata cacattcgat aagtcattga taccccaaag
     3061 aaaatgggga tattgtctta ctgcgggttc cagcagcgcg agcgccttct gaagcttcgg
     3121 tactggatcc tctccgcggg actgtgcccc atccgccgca aatgcattac agccggcggc
     3181 cacgtctcgg gcgtatggat ggctcggatc acgatcgaga acggcttgag acgcgaccaa
     3241 acactgctgg actcgctcca tcgagcttgt gccagaacct gtaacagcca tcgtaagcag
     3301 ggcggcgtag gcttttttta ggggaccggc ggtgctgtgc ggttcggcgg tggtgatttt
     3361 gtcgctgttc tcgatggcgg cggcataggc gacctccgtc ggctgaccac gggtggcggc
     3421 catttcgacc tggcgaaccc acgcctcggc aagtccttcg tagacttcgg cgtcgctctg
     3481 cccaatctgc gcggcgtctt ggtagtgccg caccgccgct gaaaacgctt ttttggcctc
     3541 ttcgtcttgg ccgctgtcgc gcgctttgag cgcccgttga agctgcatat cgccttgcag
     3601 cttgagtcct tcgtacagcc acggcgacgc ctgcaccgct gccgccgcaa gctgctcggc
     3661 tttggcaaaa tctcgctggt aataggcaat cagcgcttcc aaatagcccg gtgtgtccga
     3721 caatagcccg cggctttttt gcaacgacag aagcgccggc tgcaaaaact tcggctccag
     3781 ctctttgagc tgcttgtcgg cccactcgcc acctccggca agtcgcgctt cgtacatcgc
     3841 ttgttcgtag tgcttgccaa gcaagagccc gagcgcatag tgcacgtcag cgctgtcgtt
     3901 gccttccgcc tgggcttgcc gcaattcgga cagcgcctgc ggatattcgt gcagcgccaa
     3961 gtgaccacgc ccgagcgcat agtgcgcaag accccgtccc gagctgccgt accccgaaag
     4021 ttccgtctga agctgctgca tccgcttgcg gatgatggct ttttcgcgtt ccagatcgtg
     4081 caagggaagc tgtcgcgcac tgcgcaaaag ccactccatt ttgctgatct cttgcccgag
     4141 ccgctgcgca agctgggcct gcttttgcgc tttctcttcg cgctcaatcg tcgccagccg
     4201 ggtgcgcacg ccataccaga tcagcccgac gaggctgaga agcagcgccg acccaagcgc
     4261 caccagcggt ttgtgccgtc gagcctgccg atacagccgc tgagtcaggc caatccgccg
     4321 cgccagcacc ggctcgccgt cgatgaaacg ctgaagatcg tccgccagcg ccttcgccga
     4381 gtcgtagcgc gccggctgct ctttttccag gcacttgagc gtgatcgctt caaggtcggg
     4441 cggaatcgtc gggtcgacgg cccgcatcgg cttgggatcg acggtcagca ccgccagcag
     4501 gatgtcgatg tcaacggtcc cgtcgtatgg tgaacgccca agcagaattt cgtacagcgt
     4561 cacgccgagc gcataaacgt cggtgcgccg atccagatgc cttgtgtcac cgcgggcctg
     4621 ttcgggcgcc ataaagcgcg gcgtcccctc gatgatcccc gagcggctgt tgtccgagcc
     4681 tgtggtttcg cgcgccagac caaaatccat cacgtaggga tgccaatttc cgtcggcagt
     4741 ccgctcgacc atgatgttcg acggtttgat gtcccggtgg atcagtccct gccgatgcgc
     4801 ggcgtgcatg gcgtccgcga cttttcgcag cacctgcact ttttcaaaca gcgacatctc
     4861 gagttgggcc aaatgcagcg gcttgccact gatcagctgc atggcgatgt agggatagcc
     4921 tgaatcgtcg gtgtcgctcg gcatttcgcc gatttcgtag actttgcaga tgtgcggatg
     4981 gtccagcgac gcttgcagcc gcgcttcgcg ttcaaaacgc tttacgaaca cggctcccgt
     5041 cgatccgccc gcgtgaccga gccccggatg cacaagcttg agcgccacca gtcggttgag
     5101 ccggggatcg cgagccatgt agaccgcgcc catgccgcca gcaccgagca ctttgatgac
     5161 ttgatagcga tcccattttt gcaacgatcg cggaatgccg actttgggca tcgacggcac
     5221 cgagcgcggc gcctttgctc cgagcgatgc ggactgcgaa cccgcgaccg ccgaaaccga
     5281 ctgggtggcc ggcagatcgg tggtcggttc gtccccgtca tccggcggga gtgcgtcttg
     5341 cgaaacatgg cccccaggca ccatgtcaat ggtggcgggc tcggactctg gatcggacga
     5401 cgctacatgg gacgcgggcg gcgagcgacg atcttctgcc acagttccac tatagcaaaa
     5461 tcactccgct ttcagtgcga gccgagtcga agcacaccga agacaagctg gcagtacagc
     5521 gcgcccacca ctgccatcga cagcccccag gcaagaagct gacgatacag cttgccgtca
     5581 tccccttgcg cgttggccag acacagcgcc cccagcgtcg acagcgggct cacatcgacg
     5641 aggtgcgcac cgacgttgac gctgttggcc aaggcgacca aatcaccgcc accgagcatc
     5701 tgtatcagtt tcggcaagat cggcaaaaag gccggcagca ccacgcccga cgaactggaa
     5761 tacgccgaaa gcagccccgt gcaaaagccg atcacaaacg gtgcccgtcg ctgcgtcgat
     5821 agcttttcga taagccaggt aaacaggtcg atgccgccgg tccgttcgag gattcccatc
     5881 agacacgtca tgccgcagac catcaacatg gttccccacg gcagctcgcg caggacctgt
     5941 tcttcgtcgg acactcgcag gactgaaagc aacacggcgg cggccagtgc gacggcacca
     6001 atttcgattt ttgcgatcga caccgtgaga agcaccgcaa acagcaaaaa cagcgtcacc
     6061 gcgtgctttg tctgcagcgg cttttgttgg gacaaaagca gctcgtcaag ccgctctcgc
     6121 tgctgcggcg tcggttcggt gaagagcaac ttgccgccgc caaacagcag atatccagcc
     6181 atcgccaccg tcgtatgcgc gagcaaacac tgcgcatagt ttgaccagcc atagccaaat
     6241 agcccaatcc gccccatcag atcgctggcg atgactcccg tcggtgcaag cggcgacaaa
     6301 gacgcagcat tgccaccaca agccaccagc agcgccatca aaaaagacgg aatgccggct
     6361 tcatgcgcga ccgccatggc aacgggagcc agcatcgcaa ccgccccgat gttgccggct
     6421 ccgacggttc caagcgacag cgcgagcaga aagaaaatcg gcggcaaaag ccctggccga
     6481 ccccgcgcaa gctgcactgc atagcctgcg acccgtccga gcgtgccgtt ttgctgcgca
     6541 tgtccaaaca gcagcgtcag cgaaagcacc gtcagaaaca gcggcaccgg aaagcccgcc
     6601 atcacttggc ggatcgacat ctgggcgcca aagacgccca gcccatatgc aaacgccagc
     6661 gcgagcagcc ccacattgag ccgcgaccac atgctaagga caatcgcgca gagcagcgca
     6721 gcgacggaaa gaagcggtat gagcatcgac tctctgtagc gacccggacc agggccagtg
     6781 gatctacagt acgccgagca ggacaaactt cagatagcga ccttcgtcga acgacggcgg
     6841 caccggatga tcgagtcctt ggctgccgcg atacagcaca gtcactcgcc gtccggcatc
     6901 gagcgcagcc gcggccagca tgcgcaaaaa cgactgttca tcgacgagct gcgaacacga
     6961 acaggtcgcc aaaaggccac cgcgtcgcac cgtccccatc gccagtgcat tgagccgctg
     7021 atagcctttg agcgccgccg gcagatcctt ttgcgcgcgc gcaaacttcg gtgggtcgac
     7081 caccgtcacg tcgaaagaaa gtggcgttgc gccttcgaga aagcggaacg catcggactc
     7141 tacggtgtgc aaaagtccca ggctgttaag ctcggcgttt tcgctgcaca gcttgagcgc
     7201 tttgggcgaa atgtcgacgc aggtggcctc gacagcacca tgtcgcagcg cttgcagagc
     7261 aaaaccgccc gcgtagctgt agacatccag cacttttgcg cccgcacaga acttgccaag
     7321 cagcagccgg ttgtcccgct gatcaagaaa cgccccggtt ttttggccat cgcgcacatc
     7381 gacgcgcagc cgaatgccat tttccagaca cagcgtcccg ccggccagga ggctttcgtc
     7441 gccgaacacc acccgctgct gcgatgaaaa gccttcgatt ttggcaaaac ttcccgctgc
     7501 tgcctcgacg atcaccgcgg gcggatttgg caagctgcgc agcgccgcaa acaccgcttc
     7561 ttcgcacagc ttcatcccga gcgccgaaaa ctgcactgcc gcgacgtcgc cgaagatgtc
     7621 gacgataagt cccggcaggc catcgccttc gctgttcacc agccgatacg ccgtcgtgtc
     7681 cgaacgcggc agcccaatcg aacggcgcag caggctcgct tcgtgaatcc gccgcgaaat
     7741 cagcgatgcc acgagagccg tcggtggcag cgtgtgatcg gcgactccgg gaagcgcccc
     7801 cgcgtgcagt tcacccaggc tgagcatccg caccgcaatc tgcgatcgcg gattggcaaa
     7861 gccgtgacca atcagcttgt cgtcttggtc gtgcaccgca acgatgtcac ctggcgaaag
     7921 acccggtggc agcttctgaa tcgcccccga aaacacccac ggatgaccgc gccacacggg
     7981 attggcacga ccgcgcttta acacgacttt ggcaactggc acggcggcaa gctagcacag
     8041 cttgacctac aggccgtgga gcttctgaat ttcgatgagc tgctgacgca cgacatccga
     8101 cggcgccgtt cctgcatcgt ggcccgaagg acgatagccg ctcggatcga tcagcacgcg
     8161 cagcaccgaa agcacgctcg ccgacagcgc cgactggtgg tagccgcctt ccagcacaag
     8221 cagcattcgt gactgcacac cgagcagcga cgacaccatc tgggcatagt catcttcgac
     8281 gagcgacatc cccgcgaggg tatcgcggct gtgcccatca aagcccgccg acaccaagat
     8341 cacgtcggga gcaaaacgtc gcaagaccgg caaaacgacc cgcttaaacg ccagtttgta
     8401 ttcggggccg gcgctgcctt cgggaagcgg tatgttgacg atgcaacctc gcccgttgcc
     8461 ttcgccaatc gacgtcgctg cgcccgagcc aggaaaaaac ggaaactggt gcaggctgac
     8521 aaacaggacg ccgccgtcgc gacaaaaaat ctcttcggtg ccgttgccat ggtggacatc
     8581 gaaatcgact atcgccaccc gccgtccggt tgctcgcact gctgctgcgg ccaccgcgat
     8641 gttgttgagc aggcaaaaac ccatcgcacg atctgccgag gcgtggtgcc ccggtggccg
     8701 cacaagcgca aacccattgt cgcccggctc gcctttgctc tgggcaattc gcagcgccaa
     8761 atccgtcgct gtccccgccg catgcagagc ggcttcatac gaacccggcc cgtagtacgt
     8821 atcggggtcg atccagccct gccctttggg ctgcaaatcg cccatccgct ggcgcaggtc
     8881 gtttagatac gacgccgagt gaacacgcaa gagctcgtcc tgcgtggccc gccgcggcgg
     8941 gatgttgcgg aatcgcccga ccaaatcaga cgtgtcgatc gcggtcaaaa tcgcctgcaa
     9001 ccgctcgggc ctttcgggat gttcaccgac gagcttatgg cccagaaata cgtcaccgct
     9061 gctcaggtac gtccgcatcg ccaaatttta ccttcgcagt agacgcagaa gtcgacgaaa
     9121 aacgggcttc acttgtagtc aagcatgata ccgtagccga cctgatgtcg ccccgacgaa
     9181 gtactgaaca cctggccacg gcgcacggct cgcccgaaga tgcgcacggc cccgcggcca
     9241 agtccgcatc ttcttccacg tcgcagcagc cgtatgtgcc ctatatcgcg cccgatgcgg
     9301 atgtcgccga actgacgccc aaggccgtgc tgctcggcgc gctgtttggc atcctgtttg
     9361 gcgccgcaac tgtctacttg gcgctgcgcg caggactgac gatctcggcg tcgattccga
     9421 tttcggtgct gtcgattgcg gtgttcaaaa agctcggcaa gagcaccatc ctcgaaaaca
     9481 acatcgtgca gacgctcggg tcggcgggcg aatcgattgc cgctggtgtc gtcttcacgg
     9541 tgccggcgct tttgtttttg gcgcaaggcg acagtttttt ccgctatggc caaattctga
     9601 cgctggctgc cgtcggtggt gtgctcggaa ttttgttcat gattccgctg cgccgcagcc
     9661 tcatcgtcaa agagcacggc aagctgccct atcccgaagg caccgcgtgc gccgaagtcc
     9721 tgatggtcgg cgagcgcggc ggcgatttgg cgcgacgggt gttccacggg gtgtttgccg
     9781 cggcgggcta ctggctgctg atgggcgtgc tcaagctgtg gcgcgatacg ccggggctgc
     9841 gaaccgggcc gaagtcggtc tatcccaacg ccaacctcga gtgcaacatc acgccggaat
     9901 acctgggcgt cggctacatc gtcggtcccc gcattgccgc cgagatgact tcaggcggcg
     9961 tgctgtcctg gctggcgctg attccactga tttcgatgta cgttcccgac gcgcagcgca
    10021 tcactgactt gcacgcgctc ggctataacg atgcgtggat tacgtcccat tccgtcgccg
    10081 aacaagtcta tcgcgcctat gtccgctaca tcggtgccgg cgccgtcgcc tgtgcgggtc
    10141 ttatgacgct gcttaagacg ctgcccacca tcgtcagtag cttccgcgat tcgatccgcg
    10201 acctgcgcgg cggtcagaaa aacgaaacga ttcgcaccga gcgcgaccta ccgcttggtc
    10261 tggtggtcgt cggcacggcg gttcttgggc tgctcattgc gcttctgccg aacctgcctg
    10321 gaaaattccc cggctcgctc gtcatctcgg cgctgatcgt gctgtttggc ttcttctttg
    10381 tgaccgtcgc gagtcgcatt gtcggcatca tcggcagctc atcgaatccg atttcgggca
    10441 tgacgattgc gacgctgatg ggcacttgtc tggtcttcat caccatcggc tggcgcggcg
    10501 atgcctacca agcggcggcg ctgctcgtcg gttcggtggt gtgcatcgct tcggccaacg
    10561 cgggcgcgac ttcgcaggat ttgaagacgg gatttctcgt cggagccacg ccgtataaac
    10621 agcagatcgg cctgatgatt ggcgtcgtcg tgtcggtgct ggtgattggc ggaacgctgc
    10681 tactgctcga ccgctcgata ccgggcgttc agcacgcgat tggcacggaa aaattcccgg
    10741 cgccgcagtc gatgctgatg gcgacgatga tcaaaggcca gcttagccag cagcttccgt
    10801 ggggtccggt gctcgtcggc gtgtttatgg cgctgatggc gcagctcgcc ggagcgcacg
    10861 cgctgtcgtg ggcagtcggc gcttacctgc cgctgtcgac gacttcgcct attttcgtcg
    10921 gtggaatggt gcgttggctg gtcaatcgtc gtcgcacgct tttgtcggga ggcaaagcag
    10981 cggcggcgga atcggacatt ggaccgggca tgctgtatgc gacgggcttg gtcgcgggcg
    11041 gttctctcgc tggcatgctg atcgcgttcc ttcagggctt cgccgaaccg gtcgccaaca
    11101 agctcgacct tggacgcgac tactggaccc ggctcgggct cggtggtgac gtgattgccg
    11161 cgggcgcgtt cttcgtgctg tcggtgtttc tgattcggca agcgctgtcg tcggacaaga
    11221 agtaaccgcg ccgacggcca cgcaacacga cgacgcggct tttcaagcaa gccgcgcctg
    11281 attccacacc gcccgatcct gaatttccgt tgcgatttgc gaggggttta gcgacgcaga
    11341 cgccgggacg cgcctttttc cagacggcgg gcttcgacaa cggcacggac gtgctgaccg
    11401 cggaaaatct caccggcatc gtcgtgcgcc acgacctcga agcggtgaaa cttgccctct
    11461 aggccaaggt agcgtgccgt catgcgcacg gtgccgccaa gcggtgtcgg cgcgtggtgc
    11521 tcgacccgaa cgacaatccc cgcagtcagc tcgccgggga gcagcatcgg ctccatcgct
    11581 ttggctgcgg ccagttccat ccaggcaatc agcttggccg ttcccagcac gagcggaaac
    11641 aagttgtgct cgggaccacc gcccgtaccc gtcgccgcaa cgtgcggcgc caagtcctgc
    11701 tcgcccacga cgtgggtgat ggtcagttct tgcgctatcg atccaggccg cagcatggtc
    11761 gcttacttgg gaggatcgta ctgcttgacc gacggctgtt tctgacgcca atcagagccc
    11821 acaccaccct gcgtcgccac cgactgggtg ccgcgcttga gcatgtcgat cttggtctga
    11881 atggcatcgc cacgaaaatc gactgccggt gcgtgaacgc ccgagtccat ccggatggca
    11941 tcgcacggac aggcttcgac gcagagaccg cagaccacgc agcgcagctc gtcgatttca
    12001 aagatgcgcg ggcgcttttc gtttttgttg ccggtttctt cgggaacaat cgtgatgcag
    12061 tgcgccggac ataccgtcgg acacagcatg cacgcgacgc agcgaaccgc gccatcgtca
    12121 cgcagcatca gtcggtgaag accccggtgg cgatcggcgt atttcagctt ctgctcggga
    12181 tattcgacgg tgacggtgta cttgcgtgaa aacaggttgg cgaaaaagtg cttcgccgtc
    12241 acgccgaggc cacgaatgac ttcgggcagg tagacgcgct gttcgagaga gtcaggccga
    12301 ccgacgacgc gaacgccaac tttgccgacc ttgccactcg tctgcggagt ggtttcggaa
    12361 tgggcgggtg ggtgatgcga ggacacggct tacttgcctc ccgaccgcca cagcacaacc
    12421 gcggcggtga tgatgatgtt gagcagagac aaaggcaaaa gctgcttcca gcccagccgc
    12481 atgagctggt cgggacggaa gcgcggcagc gtccagcgaa tgagcagctg gagccagcac
    12541 aaaaacagga tcttgagcga ccaggccacg acctggatca gcaccaccgc aaggtgcggc
    12601 aggtgcaagc tgccaaagcc cgacgaatcg aggcccggaa cctgccagcc gcccagaaac
    12661 accgtcgtca cgaccgccga aaggaagatc gtctcgatga actcggccag gaagaagatg
    12721 ccgaagcgca tgcccgaata ctcgacgaag taaccgatca gctcgggctc gccctcggga
    12781 aggtcgaacg gcgcacgctt ggtttcggca atcgccgcgg tcaaaaacag cacaaacgcc
    12841 agcggctgcg tgacgatgcc ccagttgagg atgttcgagc cctgcgacag cacaatcgcg
    12901 ttcggttcga gcgagccgta gacgaggaat gcgcccatca gcgaaaggcc catgcagact
    12961 tcatacgaca tcatctgcga cgaagcgcgc agaccaccaa gcagcgacca cttgttgtac
    13021 gaagcccagc cggccagcgt cgttccgtac accgccagcg acgccagtgc gaagtaaaac
    13081 agcatgccga cgtccatccg tgcgacttgc agcggaatgg tggctttgca ctgcgacggg
    13141 tcgctcacca cgctgagcac ttggccgacg cacaggtcgg gaccaaaggg aatcaccgcg
    13201 aacgtgatca gcaccggcgc aatcgccatc accggcgcga gcgcaaacag gaagcggt
//
LOCUS       DAORGN010000141        13075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1982_length_13075_cov_6.627112, whole genome shotgun sequence.
ACCESSION   DAORGN010000141 DAORGN010000000
VERSION     DAORGN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_13075_cov_6.627112"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKL17_14760"
     CDS             <1..775
                     /locus_tag="PKL17_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNK46044.1"
                     /translation="GVFRNPLADPYVLGTVSGATFGAALTILFHLDGSLWALPLGAFF
                     GALLSTAVMLAASELRMQLYFDHGALILVGVALGSLLSALTGIVLFASRADQVQSLVY
                     WLFGSLSTGQWPQLGLFALLAIPTMGLLVRQARVLDLLSLGDAMALHLGIEVTSHKRK
                     LMLLSSLLTALSVAACGTIGFVGLVVPHIMRRLVGPGHRSLLLSSLLFGGAFLPLCDT
                     VARSLFAPVEIPVGIITALVGVPFFLSLLLRRARLGTLS"
     gene            772..1554
                     /locus_tag="PKL17_14765"
     CDS             772..1554
                     /locus_tag="PKL17_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK46045.1"
                     /translation="MISARDCGFSYGDRRVFSDLCLSVQAGELVALIGPNGAGKTTLI
                     HVLSGVKRATTGSVLISEDAGPCELGLLSPEARARKLAVVEQSVVPAFDFTVFDLVAM
                     GRYPHRHADVDHDAVVRSALDAVGVAALSDRRFAQLSARERQRVLLALALAQQPQVLL
                     LDEPLAHLDPKHQVGMMALVRKLAQGGLAVLWSVHDLNMARQADRVALLSQGRLLACG
                     PPEEVLTVPLLETAYETPVHLVPTQDGSPPLIVFGNRAASPG"
     gene            complement(1574..2965)
                     /locus_tag="PKL17_14770"
     CDS             complement(1574..2965)
                     /locus_tag="PKL17_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46046.1"
                     /translation="MNRRLSRSWFLVFPTVILTGSLVFSSSCQKKAVCDDVVGSCLAL
                     TVEGPGTYDALRTELQLPSQTRIGNTTGNIDLPLTLRVLPPSGVSSVDVSGVIVTGML
                     GGSDQAQARTPPGFAWPEGAHIEATVTLAAAGVDGGELPDGGAQDQGSPDLSNPDLAG
                     PPPTLKWTAETTAGLVRQDLYSVWSGGTNQTFAVGQSGIILTRSGTSWTQEASPTTST
                     LYAVSGFAGNGTWAGGQPVYRRGTTWTADATNLTLGVGESIWSIAPGGTFGELWVGAD
                     NGKVWRRSGATSGNGTWTVEQALPAGNRVCSVSYSGGVVLAVGQRGWVGIRRGTSSWQ
                     NYQYLDLSGSTMGNPDAFYGVYATAADSAVAVGAKGLAARFVGGTWKPGTVKIDPSGT
                     EFNAIWGSPASRFYAVGYNGVIVRVDGNLTPTALRSDGTQNLYGIFGTSETDIYVVGA
                     RNTGDALILHGTP"
     gene            complement(2962..3480)
                     /locus_tag="PKL17_14775"
     CDS             complement(2962..3480)
                     /locus_tag="PKL17_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46047.1"
                     /translation="MAFRSLWVFLFCAFVGLSTATANATVQDPDQAAKQAYARGAELF
                     KNRHYTEAATAFEEGNRLKPHPAFLYNLALTYRALGQPAKAIENYEGYLRDSPNATDR
                     ATVEAAIVEEKRKLATSQTAKSDSGSEGAPFYKKWWFWTATGAAVLALGVGLGVGLSR
                     PTTATYREVTWQ"
     gene            3610..4116
                     /locus_tag="PKL17_14780"
     CDS             3610..4116
                     /locus_tag="PKL17_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46048.1"
                     /translation="MSQMAALPQSPWFSVVGGPPPAEEVLRAVMLSLVMPLGGLCLGL
                     FLPEVWRLPCAVLSTVAMVPLFLLLRFRWRTRQQIRLFGNIIEHRDGSQVTRVALTRA
                     VVSAAAAPPGVLVMVLDDGRQQVTLARRADMQELSGLPPCLGPYLELDPEDFEIIRLA
                     THRPYAQA"
     gene            complement(4152..7445)
                     /gene="ileS"
                     /locus_tag="PKL17_14785"
     CDS             complement(4152..7445)
                     /gene="ileS"
                     /locus_tag="PKL17_14785"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNK46049.1"
                     /translation="MSSPFYAVDTKQSFPKLEEKILAFWQKSKVFEASLAERDPVTGQ
                     AKPLFVFYEGPPTANGKPGIHHVLARAFKDVIPRYKTMRGYRVPRKAGWDCHGLPVEV
                     EVEKRLGLRGKQQIEELGIAEFNKQCRESVFRYIGDWDKLTERIAFWVDTEHPYRTLD
                     NDYIETCWSLLRQLWDKNLLVRDYKVTKHCPRCGTSLAEAEAGQGMKEDVEDPSVFVR
                     LRLLPGQKLPCAPDVTSDGNVSVMIWTTTPWTLPANTAAALSEKASYGVYKIPGDDKK
                     PEEYLILLASQADTVLKGSKFECVASGILGAQLVGLRYQPLFDCHVNLLGQSVDTGNA
                     YRTLTDPTVEAGEGTGIVHIAPAYGDLEVGRRHELPVLFSVGLDGLVLPALPKFAGKF
                     FKEADPLIIRDLKDRGLLWSSGRIRHSYPFCWRCDTPLLYFAKTSWYIRTTAVREQLL
                     EQNQRIGWYPDHIRDGRFGDWLRNNVDWAISRERYWGTPLPLWICDGCGETECIGSVN
                     ELSQKVGRSISSDEAFDLHRPYVDSLAWPCPSCTGGTRRRIRDVIDCWFDSGAMPYAQ
                     HHYPFEHQNDFGELFPAAFISEGVDQTRGWFYTLHALGVLLRGGPAFENCIVLGHILD
                     DQGRKMSKRLGNIVDPWTVLHAEGADALRYYLYTASPPGQPRRFSQSLVQKSLRQFLL
                     TLWNCYSFYVTYAEAEYDRLGRDEVPLAERPQIDLWILGSLHRLLQNTTALLERYDIT
                     QAARGIADFVDILSNWYVRRCRDRFWASLDGPGGEIGGALEKDKRAAYQTLHTCLVTV
                     AQITAPFAPFVAEELWQNLKRRIDPQAELSVHLSRWPEADVVLLGQTEQLRGDMEVAL
                     KAVSLGRAARQDQNLKTRQPLKAALLQVPSAAARRALERFQAEICEELNVEELSFAAE
                     GQELVHYTLRPNLPKLGKKLGKRVKLIQDAMGQLSVDDVKKIAQSVLAEQPVTMNTTE
                     GPIELLADEVLLSAQSAEGIAARTEDGLVVGITTEITPELRDRGIARELVRGIGELRK
                     KAGCKVSDRVRIGYATSDATAQAAIVSLADYVSTETLSTLVPSLIDDADARATVELDD
                     ASIEISLKR"
     gene            7542..8555
                     /locus_tag="PKL17_14790"
     CDS             7542..8555
                     /locus_tag="PKL17_14790"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNK46050.1"
                     /translation="MSVADGAMTPPAFTPLRGHLFDRRLLIVAGKGGVGRTTVACALA
                     VLAARQGKRVLLAQTRSKNRLRELFSVERDFSEVAPVRDNLWAVNMTPESALREYGMM
                     VMHSSFLYRQVFERDVVRAFLRAVPGLHDYAMLGKTWFHTTEMSGGRPRFDVVILDGP
                     ATGHVLTLLRIPKVILDTVPEGPLTGPARDNWSLLTDPKRCLTVLTTLAEDLPVTETL
                     QLANGLKALNLSRGPVVVNRLYPPRLSAGLPKVGFDELLRSDAALSDPLLGPVLRAAR
                     LHRQRAQLNEHHVERLRRELLLPQLHLPYLFRESFGKSELDELADRLEGQVEALAVAG
                     RSV"
     gene            8793..9992
                     /locus_tag="PKL17_14795"
     CDS             8793..9992
                     /locus_tag="PKL17_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46051.1"
                     /translation="MRALAETVFRLVRRDKSQTAVHLVSRLLSELDLQQKEASESERE
                     QRAYLLNLRGVIYAQRNQEQTALADLTAANQQSPQWAVPLYNLGLCHKKARRWAEMTD
                     VLSQALAGLRRQAGRLTSTSALAQGSHLSRSVLWNLGLSQTALGKLDEARSCWQACGL
                     LSGRPGGASDLGLAQLSLPTTGPYSVERVWVQRVDPVRARILSVVRYGAACQFGDVVL
                     CDTQGQGNFYDGLTGSDGDETLPEESANGLLFIDPIESAGYALHVVQGPSATPSQAMS
                     LTERLREHGLHIEVWSLTMRLPGSSSPPEGEDRSVPLCAGLVIPAGPMKEAVPLGPIR
                     LSDGEIPSALLPNDAQRTAEKAVAVLNNAARDLGLSLYAPSLMDAAGDELGAARHRRA
                     LRRSSPA"
     gene            10100..12187
                     /locus_tag="PKL17_14800"
     CDS             10100..12187
                     /locus_tag="PKL17_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK46052.1"
                     /translation="MSKALPVAIALGLAASSLTACHSEYGDDFPTTAIMNGGDWRDEV
                     IYQVIIDRFADGDINNNHRVIPSALGRYQGGDWQGVIDHLDYLEALGVTALWISPIVK
                     NIDYDAGFDGYHGYWTQSFSKISPHFGSPAKLRELVDKAHARKMKVVLDIVTNHVGQL
                     FYYDINGNGQPDEIVTGSQSLDSVDPLARLTRADEYDPDFDRRGVQGQNGLGPAGPAN
                     IRFLNMPEINRVVPDPIRFGDGPDDVLDFSRPGVYNRRGRVTNYDRWEQLTTGDFPGG
                     LKDLDTRRGDVRRGLIRAFAEMIRRYDFDGFRIDTLKHVEHEFWQEFAPAIRKYVHGE
                     QAIYGVEPLQQPKKNWFMFGEAFDGDDALLGEFTYNSEVDSVFYFSQKFQVYQDTVKC
                     SGPTSKTAKLWNDRLRFDTSTMTPAVHGMFRNQGRFRTVDGDSLPDPVFGKPIYNQSP
                     IPGGSVDSQGMGIAPSRLLVNFLDNHDVSRFLSSIDGCYVDAKEAVRRLHNAVALMFT
                     QDGIPCIYYGTEQEFSGGNDPANRERMFDSLLNNIYRGGSTAQRENMVGSGFSRDTDT
                     FKWIAQLSSLRKSIAALRRGDLKVTWATDRTGSESDAGILAFTRTDGAKTVLVVTNVH
                     NSQTSATEFSGTQMKVPFPAGTRLVEVLGDPLDTTKEKTYLVPATGTLSVRVSSRATK
                     VLVPEADAAGLAK"
     gene            complement(12214..12864)
                     /locus_tag="PKL17_14805"
     CDS             complement(12214..12864)
                     /locus_tag="PKL17_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNK46053.1"
                     /translation="MGFEIKAVCFDLDGTLVDSEGEAADSIELALLPLGRPMTAKERD
                     FVVGHGFGEIYAFIKAAGGLPWSLTEFEEAVYKARLALFEKNGACVLPGAREVVRKLS
                     SHWPVALVTGSTRPEANLMLSALGVADCFATTLCAGEYPVGKPSPVPYLQAAERLGVS
                     PSHCVAIEDSTAGISSAKSAGMHCIGVRVGNRYGQDQSHAHHVVETLFDVATLLLG"
     gene            12991..13074
                     /locus_tag="PKL17_14810"
     tRNA            12991..13074
                     /locus_tag="PKL17_14810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13025..13027,aa:Leu,seq:taa)
BASE COUNT         2330 a         3959 c         4140 g         2646 t
ORIGIN      
        1 gggcgtgttt cgcaacccac tggccgaccc atatgtactc ggcaccgtca gtggtgcgac
       61 gttcggcgcg gcgctgacca ttttgtttca cctggacggc tcgctgtggg cgctgccgct
      121 gggggcgttt tttggggcgc ttttgtcgac ggcggtcatg ctggctgcgt cggaactgcg
      181 gatgcagctt tacttcgatc acggggcgct gatcctcgtc ggagtcgcgc tggggtcgct
      241 cttgtcggcg ctgacgggaa ttgtgctgtt cgcaagccgg gccgaccaag tgcagagcct
      301 ggtctattgg ctgttcggga gtctgtcgac ggggcagtgg ccgcagcttg ggctgtttgc
      361 gctgttggcg attccgacga tgggtctgct tgtgcggcag gcgcgggtgc tcgacctttt
      421 gtcgcttggc gatgcgatgg cgctgcacct gggcatcgag gtcacatcgc acaagcgaaa
      481 gctgatgctg ttgtcatcgc tgctgaccgc tttgtcggtg gcggcgtgcg gcacgatagg
      541 ttttgtcggt ttggtggtgc cccacatcat gcggcggctc gtcggtccgg gccatcggtc
      601 gctgctgcta tcgtcgctct tgtttggcgg ggcgtttctg ccgctgtgtg acaccgttgc
      661 gcgctcgctt ttcgcgccgg tcgaaattcc cgtcgggatc atcactgcgc tggtgggcgt
      721 gccgtttttt ctgtcgcttc tgctccgtcg ggcacgcctg gggacgctgt catgatcagc
      781 gcccgcgact gcggtttttc gtatggcgac aggcgcgtct tttccgacct gtgcctcagc
      841 gtgcaagcgg gtgagcttgt ggcgctgatt ggtccaaacg gtgctggcaa gacgacgctg
      901 attcatgtgc tgtcgggggt gaagcgggcg acgacgggtt cggtgctgat ttcggaagac
      961 gccggcccgt gtgagcttgg gcttctgtca ccggaagcac gggcgcgcaa gctggccgtc
     1021 gtcgagcagt cggtggtgcc ggccttcgat ttcacggtgt ttgatctggt ggcgatgggt
     1081 cgttatccgc accgccatgc cgacgtcgat cacgatgcgg tggtccgctc ggcgcttgat
     1141 gcggttggcg tcgccgcgct gtcggatagg cggtttgcgc agctatcggc aagggaacgg
     1201 cagcgggtgc ttttggcgct ggcgctggcg cagcagcccc aagtgcttct cctcgatgaa
     1261 ccgctcgcgc acctcgatcc caaacaccaa gtgggcatga tggcgctggt gcgcaagctg
     1321 gcccaaggcg ggcttgcggt gctgtggtcc gttcacgatc tcaacatggc gcgccaagcc
     1381 gatcgggtgg cgcttttgtc ccaaggccgg ctgcttgcgt gtggtccgcc ggaagaagtc
     1441 ctgacggtgc cgctcctcga aactgcgtat gaaacgccgg ttcatttggt tccgacgcag
     1501 gacggctcac caccgctcat cgtgtttggc aatcgagcgg cttcgccggg ttgaagcctg
     1561 ggccgggggt gaactacggt gtgccgtgca ggatgagcgc gtcgcccgtg ttgcgagccc
     1621 cgacgacata gatgtcggtt tcgctggtgc cgaaaatgcc gtataggttc tgggttccgt
     1681 cgctgcgtag cgccgtcggg gtcaagttgc cgtcgacacg aacgatgacg ccgttatagc
     1741 cgacggcgta gaaacgactg gccgggctgc cccagatcgc gttgaactcc gtgccgctcg
     1801 ggtcgatttt gacggtgccg ggcttccagg tgccgccgac aaaacgtgcg gcgagaccct
     1861 tggcaccaac ggcgacggca gaatcagcgg cggtggcata gacgccataa aacgcatccg
     1921 ggttgcccat cgtcgatccg gacaggtcga ggtactggta gttctgccag ctcgacgtgc
     1981 cgcgacggat gccaacccag ccgcgctgtc caacagccaa caccacaccg ccggaatacg
     2041 aaaccgagca cacccggttg cccgctggga gcgcctgttc gacggtccag gtgccgtttc
     2101 cagatgtcgc gccgctgcgt cgccagacct tgccgttgtc agcgccgacc cacagctcgc
     2161 caaaagtgcc gccaggagcg atcgaccaaa ttgattcgcc aacgcccagc gtcaagttgg
     2221 tcgcgtcggc agtccaggtg gttcctcgtc ggtacaccgg ctgaccgcct gcccatgtcc
     2281 catttccggc aaaaccggag acggcgtaca gcgtgcttgt cgtcggtgat gcttcttggg
     2341 tccagcttgt gccactgcgg gtgaggatga tgcccgactg accgacggca aacgtctggt
     2401 ttgtaccgcc tgaccacacc gagtaaagat cctggcgaac caggccggca gttgtttctg
     2461 ctgtccattt cagcgtcggt ggcggtccgg ccagatcggg attcgacaag tcggggctgc
     2521 cctgatcctg cgccccgccg tccggtaact cgccaccgtc gaccccggct gccgccagcg
     2581 tcaccgtcgc ttcgatgtgg gcgccttctg gccaagcaaa gccaggcgga gtgcgtgctt
     2641 gtgcttggtc gctcccgccg agcattccgg tgacgatcac gccggataca tctaccgacg
     2701 aaacgcctga cggtggcagc acgcgcaggg tcaggggcag atcgatgttg ccggtggtgt
     2761 tgccgatgcg ggtctggctg ggaagctgga gttcggtgcg cagcgcgtcg taagtccctg
     2821 gaccttcgac ggtgagagcc aaacagctac cgaccacatc gtcgcagacc gcttttttct
     2881 ggcagctcga tgaaaagaca agcgatcccg tcagaatcac cgtcggaaaa accaaaaacc
     2941 aagaacgaga cagacggcga ttcattgcca ggttacctcc cgataggtgg cggtcgtcgg
     3001 tcgcgacaga ccaacaccaa gtccgacgcc gagcgccaaa actgcggcac ccgtcgctgt
     3061 ccagaaccac cattttttgt aaaacggtgc gccctcgctg cccgaatcac tcttggcggt
     3121 ttggctcgtg gccagcttgc gcttttcttc gacaatcgcc gcctcgaccg tcgcgcggtc
     3181 ggtggcattc ggtgaatcgc gcaaatagcc ctcgtagttt tcaatcgctt tggctggctg
     3241 cccgagcgcc cgataggtca gcgccaggtt gtacagaaag gccggatggg gctttagccg
     3301 gttgccttcc tcaaaggccg tcgcggcttc ggtatagtgg cgatttttga acagctcggc
     3361 accgcgcgcg taagcctgct tggccgcctg atcaggatct tgcaccgtgg cgttcgccgt
     3421 cgctgtcgac aacccaacga acgcacaaaa cagaaaaacc caaagcgaac gaaaagccat
     3481 ctctaaaact tagctttatg aggtggttct gtcacctacc ttggcacccc atgcgacatc
     3541 gatcacacat ggccgcagcc agttttgctc ggcgctggta gactgctctt gtagctgaca
     3601 aggtccaaca tgtcgcagat ggccgcgctt ccgcagtctc cttggttttc cgtcgtgggt
     3661 ggaccccctc ccgcggaaga agtcttgcgc gccgtcatgc tgtctttggt gatgccgctc
     3721 ggtggtctgt gtttgggact gtttctgccc gaagtgtggc gtctgccgtg cgccgtattg
     3781 tcgacggtgg cgatggttcc gctgtttttg cttctgcgct ttcgctggcg gacgcggcag
     3841 cagatcaggc tgtttggcaa catcatcgaa caccgcgacg gctcgcaggt gacgcgggtg
     3901 gcgctgaccc gcgcggtggt gtcagccgcc gcggcgccgc cgggtgtgct ggtgatggtg
     3961 ctcgacgatg gcaggcagca agtgacgctg gcaaggcggg cggacatgca ggaactgtcg
     4021 gggctgccgc cgtgtctggg gccgtatctg gaactggacc cggaagactt cgagatcatc
     4081 cggcttgcca cccatcggcc ctacgcccag gcgtaagtgt ccgaagggcc gagggatggc
     4141 gcgcgacgga cttaccgctt caggctgatt tcgatgctgg cgtcgtcgag ttcgacggtg
     4201 gcacgcgcat cggcgtcgtc gatcaggctg ggtacaagcg ttgacaaggt ttccgtcgac
     4261 acgtagtcgg ccaggctcac aatcgcggcc tgcgcggtgg cgtcgctcgt cgcatagccg
     4321 atgcgaacgc ggtcggacac tttgcagccg gcttttttgc gcagctcgcc gatgccgcgg
     4381 accagctcgc gggcgatgcc acggtcgcgc agctccggcg tgatttccgt ggtgatgccg
     4441 acgaccagtc catcttcggt gcgcgccgcg atgccttcgg cggactgcgc cgacaaaagc
     4501 acttcgtccg caagcagctc gatcggtcct tcggtggtgt tcatcgtcac cggctgctcg
     4561 gccagcaccg attgggcaat tttcttcaca tcatcgacgg agagctggcc catggcgtct
     4621 tggatcagct tgacccgctt gccgagtttt ttgccaagct tgggcaggtt cgggcgcagc
     4681 gtgtagtgga ccagctcttg gccttcggcg gcaaacgaca gctcctcgac gtttagttct
     4741 tcgcagattt cggcctggaa gcgttcgagc gcccgcctgg ccgccgccga cggcacttgc
     4801 agaagcgcgg ccttgagcgg ctgccgtgtc ttcaagttct ggtcctgacg cgccgcccgc
     4861 ccgagcgaga cggccttgag cgccacttcc atgtcgccac gaagctgctc ggtttggcca
     4921 agcaggacca catctgcttc gggccagcgt gacaggtgga ccgatagctc ggcctgcgga
     4981 tcgattcgtc gcttgaggtt ttgccacagc tcctcggcga cgaacggcgc aaacggggcg
     5041 gtgatctgcg cgacggtgac caggcaggtg tgcagcgtct gataagccgc gcgcttgtct
     5101 ttttcgagcg ccccaccgat ttcgccaccc ggtccatcga ggctggccca gaatcggtcg
     5161 cgacagcgcc gcacgtacca gtttgacaaa atgtcaacga aatcggcgat gccacgcgca
     5221 gcctgcgtaa tgtcgtagcg ctcgagcagc gccgtcgtat tttgcaaaag ccggtgcagc
     5281 gaacccaaga tccacaggtc aatctgcgga cgctcggcga gcggaacttc gtctcggccc
     5341 aggcggtcgt attcggcttc ggcgtaggtg acgtagaagc tgtaacagtt ccacagcgtc
     5401 agcaaaaact ggcgcagcga cttttgaacc agcgactgcg aaaaacgccg tggctgtccc
     5461 ggcggtgacg cggtgtaaag gtaatagcgc agtgcgtcgg caccctcggc gtgtagcacc
     5521 gtccacggat cgacgatgtt gcccaggcgc ttcgacatct tgcgcccttg gtcgtcgaga
     5581 atgtgcccca gcacgatgca gttttcaaac gccggcccgc cgcgcaaaag cacgcccagc
     5641 gcatgcagcg tataaaacca gccgcgggtc tggtcgacgc cttcggaaat gaacgccgcc
     5701 gggaacagct cgccgaagtc gttttggtgt tcaaacgggt agtggtgctg ggcatacggc
     5761 atcgcgcccg aatcgaacca gcagtcgatg acatcgcgaa tccgtcttcg ggtgcccccc
     5821 gtacagcttg ggcacggcca cgcgagcgag tcgacgtaag gtcggtgcag atcgaacgct
     5881 tcgtcgctgc tgatactgcg tccgactttt tgagacagct cgttgacact gccgatgcac
     5941 tcggtttcgc cgcagccgtc gcagatccaa agcgggagcg gcgtgcccca gtagcgctca
     6001 cgagaaatcg cccagtcgac gttgttgcgc agccagtcgc cgaatcgtcc gtcgcggatg
     6061 tggtccggat accagccgat gcgctggttc tgttcgagca gttgctcgcg cacggcggtg
     6121 gtgcggatgt accagctcgt cttggcgaaa tagagcagcg gggtgtcaca gcgccagcaa
     6181 aacggatagc tgtgccggat gcgccccgac gaccacagaa ggccgcgatc tttgagatcg
     6241 cgaatgatca gcggatcggc ttctttgaag aatttgccag cgaacttggg cagcgccggc
     6301 agtacgagtc cgtcgagacc gaccgaaaac agcacgggca gctcgtgacg acgaccgact
     6361 tccaagtcac cataggcggg cgcaatgtgc acgatgccgg tgccttcgcc ggcttcgacg
     6421 gtgggatcgg tcagtgtgcg gtaggcgttc ccggtgtcca cggactgacc gagcaagttg
     6481 acgtgacagt caaacagagg ctgatagcgc agaccgacga gctgggcgcc caagatgccc
     6541 gacgcgacgc actcgaactt gctgcctttt aggacggtgt cggcctggct ggcgaggagg
     6601 atcagatatt cctcgggttt tttgtcgtcg ccgggaattt tgtatacacc gtagctggcc
     6661 ttttcactga gcgccgccgc cgtattggcc ggcagcgtcc acggcgtcgt cgtccagatc
     6721 atcactgaca cgttgccgtc ggaagtcaca tcgggcgcgc acggcagctt ttgtcctggc
     6781 agaagccgaa gccgcacgaa caccgacggg tcttcgacat cttctttcat gccctgaccg
     6841 gcttccgctt cggccagcga cgtgccacag cgcggacagt gcttggtcac tttgtagtcg
     6901 cggacgagca gatttttgtc ccacagttgc cggagcagcg accagcacgt ctcgatgtag
     6961 tcattgtcga gcgtgcggta gggatgttcg gtgtcgaccc agaacgcgat ccgctcggtc
     7021 agcttgtccc agtcgccgat gtagcggaac acgctctcgc ggcactgctt gttgaattcg
     7081 gcgatgccca gctcttcgat ctgctgtttg ccgcgcagac cgagccgttt ttcgacctcg
     7141 acttcgacgg gcagaccgtg gcagtcccag ccggctttgc gcggcacccg ataccctcgc
     7201 atcgttttgt agcgcgggat cacgtctttg aacgcgcggg caaggacgtg gtggatgccg
     7261 ggcttgccat tggccgtcgg tggtccctca tagaacacga acagcggctt ggcctggccg
     7321 gtcacaggat cgcgctcggc gagactggct tcgaagactt tgctcttttg ccagaaagcg
     7381 aggatcttct cctcgagctt gggaaagctc tgtttggtat cgacggcgta aaagggcgaa
     7441 ctcatggtgg cgcgcagcat accagtttgt tgcgaaagtg gagtctcccc gagccaccct
     7501 cgttgacacg ccacgggccg gtcgctaccc tcgcagccgt aatgtcggtt gccgatggcg
     7561 cgatgacgcc gcccgctttc acaccactgc gcggtcacct gtttgaccgg cggctgctta
     7621 ttgtggcggg caaaggtggg gtgggacgga cgacggtggc ttgtgcgctg gccgtgctgg
     7681 cggcgcggca gggcaagcgg gtgcttttgg cccaaacccg cagcaaaaat cgcctgcgcg
     7741 agctgttttc cgtcgagcgt gacttttccg aagtggcgcc tgtgcgcgac aacttgtggg
     7801 cggtcaacat gacgccggaa tcggcgctgc gcgaatacgg gatgatggtg atgcactcat
     7861 cgtttctgta tcgccaggtg ttcgagcgcg atgtggtgcg ggcgtttttg cgggcggtgc
     7921 cggggcttca cgactacgcg atgctcggca agacgtggtt tcacaccacc gagatgtccg
     7981 gcggcaggcc gcgtttcgat gtggtgattt tggatgggcc agcgacggga catgtgctga
     8041 cgttgttgcg cattccgaaa gtgatcctcg ataccgtgcc cgagggaccg ctgacggggc
     8101 cggcgcgcga caactggagc ttgctgaccg atccgaagcg ctgtctgacg gtgctgacca
     8161 ccctggccga agacctgccg gtgaccgaga cgctccagct tgcaaacggg ctcaaggcgc
     8221 tgaacctttc gcgggggccc gtggtggtca atcggctgta tccaccgcgg ctgtcagcgg
     8281 gcctgcccaa agtcggtttc gacgagctgc tgcgctcgga tgcggcgctt tctgatccgt
     8341 tgcttgggcc ggtgctgcgt gcggcgcggc ttcatcgtca gcgtgcgcag ctcaacgaac
     8401 accacgtcga gcgtctgcgg cgagagcttt tgctgccgca gctgcacctg ccgtatctgt
     8461 ttcgcgaatc ctttggcaag tcggagcttg acgagctggc cgaccggctc gaaggacaag
     8521 tcgaagcact cgcggtcgcg ggccgcagcg tctagcccga ccgggtttcc ttcgcgggcg
     8581 gtgggccaaa aagacccggc tgttggcggg gcattgggca gttttgcgcc aagtcgaatc
     8641 gcgaaaagcc ctgcgaattt gtagatttgt atttttagac accaaaaatt agttggtttt
     8701 tggtctagaa acgcccgtcg gtttcaaagt acgctatccc catgggaccc cggtcgcttg
     8761 cgtgcatgct ccctcaggag ctggtgccgc aaatgcgtgc gcttgcagaa accgtcttcc
     8821 gccttgtccg tcgcgacaag agccagaccg ccgttcatct cgtgagccgt ttgctcagtg
     8881 agctggacct ccaacaaaaa gaggcgagtg aaagcgaacg agaacagcgc gcttatcttc
     8941 tcaacctgcg tggcgtgatt tatgcgcagc gcaaccagga acagacggca ctggccgacc
     9001 ttacggcagc caaccagcag tcgccgcagt gggcggttcc gctttacaac ctggggctgt
     9061 gtcacaaaaa ggcgcggcgc tgggcggaaa tgacggatgt tctgtcgcaa gcgctcgcgg
     9121 gattgcgacg gcaggccggg cggctgacca gcacgagcgc acttgcgcaa ggcagtcacc
     9181 tgagtcggtc ggttttgtgg aacctggggc tttcgcagac ggcgctcggc aagctcgacg
     9241 aagcgcggtc gtgctggcag gcctgcggtc ttctgtcggg tcgaccgggt ggcgccagtg
     9301 atctggggct tgcgcagctg tcgctgccga cgaccggacc ctattcagtc gagcgcgtgt
     9361 gggtgcagcg tgtcgatccg gtgcgggcgc ggattctgtc ggtggtgcgc tatggtgccg
     9421 cctgtcagtt tggtgatgtc gtgctgtgcg atacgcaagg gcagggcaat ttttacgatg
     9481 gactgactgg ttccgacggt gacgaaacgc tgccggaaga atcggccaat gggcttttgt
     9541 tcatcgatcc gatcgagtca gcaggttacg cactgcatgt ggtgcaaggt ccgtcggcca
     9601 ctccgtcgca agcgatgtcg ctgaccgaac ggctgcgcga acacggcctg cacatcgaag
     9661 tgtggtcgct gacgatgcgt ctgccgggca gcagctcgcc gcccgagggt gaagatcgca
     9721 gcgtgccatt gtgcgcgggc ctggtgattc cagcgggacc gatgaaagaa gcagtgccgc
     9781 tcggtccgat tcggctgtcc gacggggaaa ttccgtcggc gcttttgccc aacgatgcgc
     9841 aacggacggc ggaaaaagcc gtggcggtgc taaacaatgc ggcgcgcgat ctcggtctgt
     9901 cgctgtatgc accatcgctg atggatgcgg caggggatga actcggtgcg gcgcgacatc
     9961 gccgggcact gcgccgcagc agtcccgcgt agtccatcta tagcgaaccc cacttctcaa
    10021 actctctgtc tgctgtcggt gttttgcctc ttggcggtct gacctgcttc gcgctaggtt
    10081 ccggcctcac gagggaaaca tgtcgaaggc cctgccggtt gcgatcgcac ttggacttgc
    10141 tgcttcgtcg ctgactgctt gtcacagcga atacggcgac gactttccga ccaccgccat
    10201 catgaatggt ggcgactggc gcgacgaagt catctatcaa gtcatcatcg atcgctttgc
    10261 cgacggtgac atcaacaaca accaccgcgt cattccaagc gcactcgggc gctatcaggg
    10321 gggcgactgg cagggcgtca tcgatcatct cgactacctc gaggcgctcg gggtgacggc
    10381 gctgtggatt tcgccgatcg tcaaaaacat cgactacgac gcgggcttcg acggctacca
    10441 cggatattgg acgcagtctt tttccaaaat cagtccccac ttcggatcgc ccgccaagct
    10501 gcgcgagctc gtcgacaaag cccacgcccg caagatgaaa gttgtgctcg acatcgtgac
    10561 aaatcacgtc ggccagcttt tctattacga catcaacggc aatgggcagc ccgacgaaat
    10621 cgtgacgggg tcgcagtcgc tcgatagcgt cgatccgctg gctcgtctga cgcgcgccga
    10681 tgaatacgat ccggacttcg atcgccgcgg cgtccagggt caaaacgggc ttgggccagc
    10741 cggaccggcg aacattcgct ttctcaacat gccggaaatc aaccgcgtgg tgcccgaccc
    10801 gatccgcttt ggcgacggcc ccgacgacgt actcgatttt tccaggcccg gtgtctataa
    10861 ccgtcgcggt cgcgtcacaa actacgatcg ctgggaacag ctcacgacgg gagatttccc
    10921 cggcgggctc aaagaccttg atacgcggcg cggcgatgtg cggcgcggcc tgatccgggc
    10981 gttcgccgag atgatccgtc gctacgattt tgacggcttc cgcatcgaca ccctcaagca
    11041 cgtcgaacac gagttctggc aggaatttgc ccccgctatt cgcaagtacg tccacggcga
    11101 gcaagccatt tacggcgtcg agccgctcca gcagccgaag aaaaattggt tcatgttcgg
    11161 cgaagccttc gacggagacg acgcgctgct cggcgagttt acctacaaca gcgaagtcga
    11221 ctcggtcttt tacttttcgc aaaaattcca ggtctaccaa gacaccgtca agtgcagcgg
    11281 tccgacgagc aagacggcga agctgtggaa cgatcgactg cggtttgata cctcgacgat
    11341 gaccccagcg gtgcacggaa tgttccgcaa ccagggtcgc tttaggaccg tcgatggtga
    11401 ttcgctgccc gatcccgtct ttggcaagcc gatctacaac cagtcgccga ttccgggtgg
    11461 ctctgtcgac agccagggaa tggggattgc gccgtcgcgg ctgctcgtca acttcctcga
    11521 caaccacgac gtttcgcgct ttttgtcgtc gatagacggc tgctacgtcg atgccaaaga
    11581 agccgtccgc cgtctgcaca atgccgtcgc gctgatgttc acccaggacg gcatcccgtg
    11641 catttactac ggcaccgagc aggaattttc cggcggcaat gacccggcga accgcgagcg
    11701 gatgttcgac agcctgctaa acaacatcta ccgaggcgga tcgacggcgc agcgtgaaaa
    11761 catggtggga tcgggctttt cgcgcgacac cgacaccttc aagtggattg cccagctttc
    11821 gtcgctgcgc aagtcgattg cggccctgcg acgcggcgat ctgaaagtta cctgggcgac
    11881 cgaccggacc ggcagcgaaa gcgatgctgg catcctcgcg ttcacgcgca ccgacggagc
    11941 caagacggtt cttgtcgtca ccaacgtcca caacagccag accagcgcga ccgagttttc
    12001 cggcacccag atgaaagttc cattcccggc gggcactcgt ctcgtcgaag ttctgggcga
    12061 tccgctcgac accaccaaag aaaaaacgta tctggtgcca gcgacgggaa cgctttcggt
    12121 ccgcgtgtca tcgcgcgcga ccaaagtgct ggttcccgaa gcggacgccg ccggactcgc
    12181 caaatagcgc caaagctgcg cgggcgagcg gggtcagcca agcaacaggg tggcgacgtc
    12241 gaacagcgtt tcgacgacgt ggtgggcatg cgactgatct tggccgtagc gattgccgac
    12301 gcgcacgccg atgcagtgca ttcctgctga ctttgccgaa gagatgcctg ccgtcgagtc
    12361 ttcaattgca acacagtgcg acggcgaaac cccgagtcgc tcggcggcct gcaaataggg
    12421 cactggcgac ggcttgccaa ccggatattc gccggcgcac agcgtcgtcg caaaacagtc
    12481 cgcgacgccg agcgcgctca gcatcaggtt ggcttctggc cgcgtcgaac ccgtgacgag
    12541 cgcaacaggc caatgcgacg acagctttcg caccacttcg cgcgcaccgg gcagcacaca
    12601 ggcaccgttt ttttcaaaca gggcgagccg cgctttgtac accgcttcct cgaactcggt
    12661 cagcgaccac ggcaagccac ccgccgcttt gatgaaagca taaatttcgc caaagccatg
    12721 cccgacgaca aaatcacgct ctttggcggt catgggccgg cccagcggca aaagcgccag
    12781 ctcgatcgag tccgctgcct ctccttcgct atcgaccagc gtcccgtcga gatcgaaaca
    12841 cactgccttt atctcaaaac ccatacccat cctgtttgtt gtcggaaaaa atcggtcccc
    12901 gccgcccaca gcggtccttc acactaaacg ctacgtcaaa gagaccaaaa atttgttatc
    12961 tatttgccgc cgttaacaat cggctcgtcg gcccgagtgg cgaaataggt aaacgcagag
    13021 gacttaaaat ccttagccgt aactggcgtg tgggttcgat tcccgcctcg ggcac
//
LOCUS       DAORGN010000142        13031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_1990_length_13031_cov_5.896270, whole genome shotgun sequence.
ACCESSION   DAORGN010000142 DAORGN010000000
VERSION     DAORGN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1990_length_13031_cov_5.896270"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKL17_14815"
     CDS             complement(<1..203)
                     /locus_tag="PKL17_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46054.1"
                     /translation="MFAPTCARLFLVGAVLLFSPSLFAARLAVLAPKVDADSPVSEGR
                     RNKMHDALAQGLSDVAGADFTVLS"
     gene            complement(285..917)
                     /locus_tag="PKL17_14820"
     CDS             complement(285..917)
                     /locus_tag="PKL17_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK46055.1"
                     /translation="MTCRSLPLASHNTPSQPLGQLAHAALRTDEQSWRKLRDLGLRLC
                     DGRLHEIRACPDCHGDISREIDAVTAIRMLVGRLKSHAVPDVLVASAQVLADWAAHSG
                     LRSSPATQNESAEALAVIRIPVGTALVEAERQLLVAALNWCGGNRSETAKLLGLSRRT
                     LYNKLQSLKRRKLLSDPLPPARSGHRTRVIHAKKHVVQPPAEQPANPTAA"
     gene            complement(1205..1345)
                     /locus_tag="PKL17_14825"
     CDS             complement(1205..1345)
                     /locus_tag="PKL17_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46056.1"
                     /translation="MKTVVAFVMVSLMGLAACGPKAQTAPANAAPAPAPAPATPAGAP
                     PP"
     gene            1592..2560
                     /locus_tag="PKL17_14830"
     CDS             1592..2560
                     /locus_tag="PKL17_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK46057.1"
                     /translation="MIEVEDLTKYYGGKRAIHDLCFQIEKGEIVGFLGLNGAGKSTTL
                     RILGCLILPSSGVVRIDGLDLAEGAHEIRKRLGFLPDTPPLYDEMSVQDYLRFVAELK
                     GVGSKNLTRYIGDALSICNLADVAADNISTLSHGYRQRVGLAQSLVHKPSLLILDEPT
                     SGLDPVQIVEMRELIKRLGGEHTVLLSSHILTEISQTCDRLLVIDRGKLVADGTEESI
                     SQQAHGSAQGGVLEVELEGEGDAVADQARQAIGRVQGVIVARVVSKQARRVRLEVECS
                     DDRRAQVARAVVESGLGLLHLQRKALELESIFQRLVHPSHPQPPAT"
     gene            2601..3332
                     /locus_tag="PKL17_14835"
     CDS             2601..3332
                     /locus_tag="PKL17_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK46058.1"
                     /translation="MKPIALIIRRELAAYLRSPLGWVVAAVLLLIDGLAFNVFAVGSD
                     TARKSSEVISQFFFFSGGLAEAAGLFLAMRLIAEERQNGTINLLFSSPIRDYQIVLGK
                     FFAAFIFLLGIHVVSVYMPLMVLLHGKVAKGHLVAGYIGVLLMGSTGLAIGMFASSLS
                     KSQLVAIVVGAAISVALLLSWLLAPVTDRPFDQFFSWLALWNKHFPSFMRGSISLQDV
                     AYYLSMTYVFLFATTRVLESRRWSS"
     gene            3335..5281
                     /locus_tag="PKL17_14840"
     CDS             3335..5281
                     /locus_tag="PKL17_14840"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNK46059.1"
                     /translation="MAEKRTSGGHRPKSIQVVLWPTVLFFVGLFVLFLGERMLTTEGP
                     QKAAAILSALLLVAGFVGLLSRRGSAKDAEEKRALGYMMLSGLLVIVGVVVYLLFHFK
                     SPGLQESLRSSLGKNYEKSASMAQVFLPMMVLLGVLPLAFIQQAIYSMTDGQGGTEAI
                     EPHRVSYAVQSGLSVALVAIFIAAGNYVVTERNTKIDLARFQNTKPGETTRKVVQSLS
                     RPIQATLFFPTANEVREQVQPYFDELGKLSPNFQVQILDHALEPVRAKEMSVSGNGMI
                     VLTQLDEKGKPGSRETINIGLSVDTARGQLLAFDSDVQKKLLLLSRPGRVVYFTSGHG
                     ERSFDTGAMDLMKDDLRLPVGALRTMMVNLGYEVRSLSVQNGLAGRLPQDAGLVVIAG
                     PTERFLAEEVSALSTFIEEGGHLLLLLDPTAEQTAVDLAPVLKKVGLKYTPQVLCHPE
                     KFAVRTHKDNDRENIVSTSFSSHVSVTTLSRNSGRAGVILPKSGFFEREPVMPAGVQL
                     DFTLRSMPNTFADTNRNFQLDAGEKSQVFELAAVAQKLLDPGKDGKSKRELRLVALGS
                     ADAMSDGLVPWLPNRVLIMDSVKWLMQEEAILGEPVQDQDVPIVHTKGQEKLWFWLTT
                     LLLPSFVLGVGLLYTRSVRRRRSS"
     gene            5278..6579
                     /locus_tag="PKL17_14845"
     CDS             5278..6579
                     /locus_tag="PKL17_14845"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNK46060.1"
                     /translation="MKTVKGQQGLSPVYVHAVLATLAVGFAYQTWTRDRTQTQTDSIV
                     VLDVAKKEVSSLTYEDETKQVIVQRKTGTDGEPYAWLVTRTKSKVPVNPPAPPTPLAP
                     PAAVAPAPTAAPAVPIKAGAFPTAKPAAPPVKGGDKSGEPAVPPGHPPLPLAGPAPAA
                     APTSVAPAVAAPAPTPPPVEMKEITTVKEYRGNDGAVELLGLFGPLKALRTLGAVDEA
                     KAKELGFSGSKKTLTVSAKGLSYAFVLGGTSYGSGDYYVRDPQGQVYLLSQRIIADFE
                     FSDSRLMERRLHRFERADIDRVEVTVGTKTRTFVQGSRQNPQAFFFAEQATPEKRDDS
                     IKNWMEKLLRLAISDYVAQGEDPSTGAGASSISGAAKVGDVMTARFFDGRKEIGQVML
                     SRYPNAKTNQPEYYARTETTIGQVHLLAATAESVVQDAEKW"
     gene            6629..8479
                     /locus_tag="PKL17_14850"
     CDS             6629..8479
                     /locus_tag="PKL17_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNK46061.1"
                     /translation="MNVLFEDGGVLRAAALLSEQAGAIQVEFPGGKREKVRADRVLLH
                     FAKPLPLELLTDGQKLATEVDREFLWEVAGDAEFAFSDLAREYFGHPPTAIESAAMLT
                     VLYGSPSHFSRRGVGRFQAVTMEARQAAEKAAARKQKQLEKQTALADQLIAGELPRDI
                     AARMPQVLYKPDPSWIETKAILQACDATHMSPAKLLIRCGAVDGSHSFHLGRFLLDYF
                     PKGRSIETTATVDPLPDLPLADVAAFSIDDTSTTEIDDAFSVTRQRDGSVRVGIHIAA
                     PALGFQPGSALDELARSRLSTVYMPGDKITMLPPQAIKPFTLLEGHDCPAVSLYLTVS
                     GDGSYRILGEESRLERVPIRRNLRYPAIDSQFSVENIATGRLDFEFGPELLFLYELAG
                     RLQASRGKPEQEQVDYIFSFVDGKIALTRRMRGGPPDKVVAELMIYVNSAWGKLLGDR
                     DIRALYRVQDSGKVRLSLYPAPHAGLGVTQYLWSSSPLRRYCDLVNQWILIARLYEEP
                     SPYADNEGELLKLMRDFEQTYGAYDEFQRSMERYYGIRYLAQEGITQTTATVIRDNLV
                     RFDLVPIWQRVPSLPELPAGTSVVVDVAHLDELELTLHAEFRRKSDGAVS"
     gene            complement(8482..9243)
                     /gene="truA"
                     /locus_tag="PKL17_14855"
     CDS             complement(8482..9243)
                     /gene="truA"
                     /locus_tag="PKL17_14855"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNK46062.1"
                     /translation="MPTYRLHICYDGTDFCGWQRQLNGPSVQAALEEALGKVLKEPVI
                     CRAAGRTDSGVHALGQVVSIRLPREIPEKALVYGTNNHLPDSVAVVSAQLVDDSFDAR
                     HSSSGKLYRYQIWNGNIRSPLHCRNHWHVGFPLDLEKMRAAAEVLVGRHDFRAFRAAD
                     CERETTVRTMKRIDVLVPPHDPAAIYIEVEGTAFLKNMVRILSGTIVAAGRGLRSTEE
                     IATLLKTGDRTRAGQTAPACGLLLVRVDYGKREGP"
     gene            9404..10429
                     /gene="gap"
                     /locus_tag="PKL17_14860"
     CDS             9404..10429
                     /gene="gap"
                     /locus_tag="PKL17_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNK46063.1"
                     /translation="MAVRIGINGFGRIGRCVVRAWQKDAAKAGYEIVAINDLTDTPTL
                     AHLLKYDSVHGRFDGNVTVDGNNIVVDGKPIVVTAEKDLTKLDWRKHGVEIVLECTGV
                     FTDAPKARTHIDVGGAKKVLISAPAKGPDITIALGINQDQYQHDKHHVISNASCTTNC
                     LAPVAKVLLDNFGIVSGMMTTIHSYTNDQRILDLPHKDLRRARAAALSMIPTTTGAAK
                     ALSEVIPALKGKLDGYAMRVPTPNVSVVDFTVRLEKPATVEQINAAFRAAAENPAFRG
                     VLAVSSEPTVSCDYNGDPHSATVDLGSTQMVGDMAKVLAWYDNEMGYSNRLYELAKLV
                     SEKSQSA"
     gene            10448..11659
                     /locus_tag="PKL17_14865"
     CDS             10448..11659
                     /locus_tag="PKL17_14865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNK46064.1"
                     /translation="MATIRKVSELDVTGKRVFVRVDFNVPQNDKGEITDDTRIRAALP
                     TIRNLVERGARVILGSHLGRPKGKTPSLTMLPVGQRLAELLAPQVKEIKLCDEVVGDG
                     VRKVIADLRDGEIALLENLRWEPGEEKNDETLSKALASLAEVYVNDAFGTAHRAHCST
                     AGMVKHFAPGKRGAGFLMLREVEFLSRLLHNVERPYVVVIGGAKVSDKINVLTNLASC
                     ASSVLIGGAMANTFLAAEGKNLGKSKLEADKLQVAKQFLDRCARDGVDIVLPTDLVVA
                     PSIDAAEGTVVAVDAVPSDQMALDIGPASAREFASLIREAKTVFWNGPLGLFEKKPFA
                     AGTFAIARALAECAGTTVVGGGDSVAAVSDAGVESKVSHVSTGGGASLEFVEGQTLPG
                     IAALYEPESAQ"
     gene            11656..12435
                     /gene="tpiA"
                     /locus_tag="PKL17_14870"
     CDS             11656..12435
                     /gene="tpiA"
                     /locus_tag="PKL17_14870"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNK46065.1"
                     /translation="MSRSALIVGNWKMYKTVAEGLELATAVKNAVAPTVRGREVAVAI
                     APPFPSLYPIGKRLEGSTVALAAQDCFWENQGAFTGEVSAAMLADVGCKFVIIGHSER
                     RQHFGETDAAVNLKAKAVLKAGLLPIVCVGETEKERDAGEMRGRIIAQLAEGLFGFTP
                     DMMQRTVIAYEPVWAIGTGRVATTAQAQEVHALIRQYLHAQLGEVAGSVRILYGGSVK
                     PNNIAGLMAQPDVDGALVGGASLRAEEFLQIVRFHEAPDAT"
     gene            12432..>13031
                     /gene="secG"
                     /locus_tag="PKL17_14875"
     CDS             12432..>13031
                     /gene="secG"
                     /locus_tag="PKL17_14875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNK46066.1"
                     /translation="MMTLLYTIHVLVCLFLMLVILLQAGKGGGMGALGSGATQQVFGG
                     RGASNFLERLTTATAVVFMLTAVTLSYINSQSESDKLAKLSAKKKSDEKSAKKKAEDS
                     LKQQVEKRAAEEKAAEQKALEEKLKAAAAAAAAATSDGGADASTGAAVPGVPSAAPTA
                     PGAAPAAQPGAPKLTVTATPPAAPTAAKPATGVPAPVANP"
BASE COUNT         2553 a         3663 c         4211 g         2604 t
ORIGIN      
        1 gacagcaccg taaagtcagc gcccgcgaca tcgcttaggc cctgcgccag cgcgtcgtgc
       61 attttgttgc ggcgtccctc ggaaaccggt gaatctgcat cgacttttgg ggcaagcacg
      121 gccagtctcg ccgcaaacag cgacggggaa aacaaaagga ctgctccgac gagaaacaga
      181 cgggcacaag ttggggcgaa catgcggcca gtctagcatg gcctttctac aagggaaggt
      241 gtctgctccc tcgggttcgg cattttaaat gggaagcaaa ccgctcaagc tgcggttggg
      301 tttgctggct gttcggctgg cggctgaacg acgtgctttt tggcgtgaat gacgcgggtt
      361 cgatgtcccg accgcgctgg tggcaaggga tcgctcagca gcttgcgtcg cttcagactt
      421 tgcagcttgt tgtagagcgt tcgcctcgac aaaccgagca gcttggcggt ttcactgcga
      481 ttgcctccgc accagttcag tgccgcgacg agcagctgtc gttctgcttc gacaagcgct
      541 gttcccacgg gaattcggat cacggcgagc gcttctgcgg actcgttttg ggtggctggg
      601 gaagatcgca agccgctgtg cgcggcccag tcggccagaa cttgagcgct cgcgaccagc
      661 acgtctggaa ccgcgtggga tttcagccgt ccgaccagca ttcgtatcgc cgtcactgca
      721 tcaatttcgc ggctgatgtc gccgtgacag tctgggcatg cgcgaatttc gtgtagcctt
      781 ccatcacaca ggcgcagacc gagatcccgt agttttcgcc aggactgctc gtcggtgcga
      841 agggcggcgt gggcgagctg acctagcggc tggctcgggg tgttgtggct ggcaagcggt
      901 agcgaacgac aagtcatgga tgcctcgtgg acagaccggg gatttgtccg ggcctggatt
      961 gcaggcacca tgccaagcca cttgcgatgt ggtcgtcctg gctggttttc gtcggaaagc
     1021 gagtgcggaa atcgtggaga tggcgcgatc tggctacaag ccaaagtcgg tcacggtccg
     1081 gctcaccgga cttggtcagt ccgcagtggg caaaaaaaaa gagcctgcca ctacgagagt
     1141 gacaagctct ttcttggacc ttcgcaagcg attgctcaca gaaggcccgg ctcacccacc
     1201 gaaattacgg agggggggcg cccgccgggg tcgccggagc cggagcaggg gccggagcag
     1261 cgttggccgg ggcggtctgg gccttgggtc cgcaggcggc cagccccatc aggctgacca
     1321 tgacgaaagc aactaccgtc ttcattggat tctccttttt ctggaaggga gcgcgcacct
     1381 tgcgcgaagc gggatggtat gcagaccttt ccgtagggtc aagtccccta aacatctggc
     1441 cggcaagtgc tgtgcttatg gggggttgaa atggccgttg ggtatgcgta tcttgccgcg
     1501 cacattccgg gcttttgcca aattgggcca gctcgccgtc gcggcgggaa cacaaaaaac
     1561 cgtagctgag attcggtgga gacactgctc gatgatcgaa gttgaggacc tcacgaaata
     1621 ctacggaggg aagcgggcga tacacgacct gtgcttccaa attgaaaagg gcgagatcgt
     1681 cggtttcctc gggctaaacg gagccgggaa atcgacgacg ctgcgcatct tgggctgttt
     1741 gatccttccg tcgtccggcg tagttcggat cgacgggttg gatctggcgg aaggcgcgca
     1801 cgagattcgc aagcggcttg gttttttgcc tgatacgccg ccgctgtacg acgaaatgtc
     1861 ggtgcaagac tacctgcgtt tcgtcgctga gctaaaaggc gtcggcagca aaaacctaac
     1921 gcgctacatc ggggatgcac tgtcgatttg caatctggcc gatgtggctg cggacaacat
     1981 ttcgacgctg tcgcacggtt atcggcagcg cgtcggcctg gctcagtcgc tggtccacaa
     2041 gccatcgctg ctgatcctgg atgaaccgac gagcggactc gatccggttc agatcgtcga
     2101 aatgcgcgag cttatcaagc gactcggcgg tgagcatacg gtgctgctgt cgagccacat
     2161 cctgaccgaa atcagccaga cctgtgaccg actgctcgtc atcgatcgag gaaagctagt
     2221 tgccgacggt acagaagagt cgatttcgca gcaagcccac ggcagtgcgc aaggtggcgt
     2281 gctggaagtc gagcttgaag gcgaaggcga tgccgttgct gaccaagcgc gtcaggcgat
     2341 cggtcgggtg cagggggtta tcgttgcgcg cgtggtgtcg aaacaggcgc ggcgcgtgcg
     2401 actcgaagtc gaatgcagtg atgacagacg tgcccaggtg gcgcgggctg tcgtcgagtc
     2461 tggcctgggt ctgctccatc ttcagcgcaa agcgctcgaa ctggaatcga tcttccagcg
     2521 cctggttcat ccctctcacc cacagcctcc agcgacgtga ggccttttga aatgtcaact
     2581 gaagctctga ggtggctgcc gtgaaaccca ttgctctcat cattcgacgc gagctggctg
     2641 cgtacttgcg atctccgctt ggctgggtcg tggcggcggt gttgctgctc atcgatggtc
     2701 tggcgttcaa cgtctttgcc gtcggaagtg acaccgcccg aaagtcgtcc gaagtcatca
     2761 gccagttttt cttcttttct ggcggtctgg ccgaggctgc aggcctgttt ttggcgatgc
     2821 gtcttatcgc tgaagaacgc caaaatggaa ccatcaacct gcttttctcg tcgccgattc
     2881 gggactacca gattgtgctc ggcaagtttt ttgccgcgtt catcttcctg ctcggcattc
     2941 atgtggtgtc ggtgtacatg ccgctgatgg tgctgctcca cggcaaggtg gccaagggcc
     3001 atcttgtcgc cggctacatt ggcgtgctgc tgatgggatc gacgggcttg gcgatcggga
     3061 tgtttgcgtc gtcgctcagc aagagtcagt tggtcgcgat tgtcgttggg gctgcgattt
     3121 ccgtcgcact gcttctgtcg tggctattgg ctcccgtcac ggatcgtccg tttgaccagt
     3181 tcttttcgtg gctggcgctg tggaacaaac acttcccgtc gttcatgcgc ggatcgatca
     3241 gcctgcaaga cgtcgcctac tacttgtcga tgacgtatgt ctttttgttt gcgacgacgc
     3301 gagtgcttga gtcgcgacgg tggagcagct agccatggcg gaaaagcgaa ccagcggcgg
     3361 tcatcgcccc aagtcgattc aggtggtgct atggcccacc gtgctctttt ttgttggctt
     3421 gtttgtgctg ttcctcggtg agcggatgct gacgacggaa ggtccgcaga aggcggcggc
     3481 gattctgtcg gcgctgctgc tcgtcgccgg cttcgtcggg cttctgtccc gtcgtggatc
     3541 ggccaaagac gcggaagaaa agcgagcgct cggctacatg atgttgtcgg ggctgctcgt
     3601 catcgtcggt gtcgttgtgt acttgctgtt tcacttcaag tcgccgggtc tgcaagagtc
     3661 gctgcgatcg agcctgggca aaaactacga aaagtcagcg agcatggcgc aggtgttcct
     3721 gccgatgatg gtgctgcttg gcgtgctgcc gctcgcgttc atccagcagg cgatttactc
     3781 gatgaccgac ggacagggcg gcaccgaagc catcgaaccg caccgcgttt cgtatgccgt
     3841 gcagagcggc ttgtcggtgg cgctggttgc gatctttatc gccgcgggca actacgttgt
     3901 caccgagcgc aacaccaaga tcgatctggc gcgcttccag aacaccaagc cgggggaaac
     3961 gacccgcaag gtggtgcaga gcctgagccg tccgatccaa gcgacgctgt ttttcccgac
     4021 ggcaaatgaa gtgcgcgaac aagtgcagcc gtactttgat gagcttggca agctgtcgcc
     4081 gaacttccaa gtgcagatcc tggatcatgc gctggagcct gtgcgggcca aggaaatgtc
     4141 ggtgtcgggc aacggcatga ttgtgctcac gcagctcgac gaaaagggca agccgggcag
     4201 tcgtgaaacg atcaacattg gtctgagcgt cgacacggcg cgtggtcagc ttctggcgtt
     4261 tgattccgac gtgcaaaaga agctgctgct gctgtctcgt ccgggtcggg tggtgtactt
     4321 cacaagtggc cacggcgagc gatcgtttga taccggcgcg atggacctga tgaaagacga
     4381 tctgcggctc cccgtcggag cgctgcgcac gatgatggtg aacctggggt acgaggtccg
     4441 ttcgctgtcg gtgcaaaatg gtctggcggg acggctgccg caggacgcgg ggctggtggt
     4501 gattgccggt ccgactgagc gctttctggc cgaagaggtg agtgcgctgt cgacgttcat
     4561 cgaagagggc ggccacttgc tattgctgct tgatccgacg gcggaacaga cggcggtgga
     4621 tctggcgccg gtgctaaaga aagtggggct taagtacacg ccgcaggtgc tgtgccatcc
     4681 ggaaaaattc gcggtgcgca cgcacaaaga caacgatcgc gaaaacatcg tctcgacgag
     4741 cttcagctcg cacgtatcgg tgacgacgct gtcgcggaat tcagggcggg ccggggtgat
     4801 tctgcccaag agcggcttct tcgagcgtga gccggtgatg ccagcgggcg tgcagctcga
     4861 cttcaccctg cggtcgatgc caaatacgtt cgcggataca aaccggaact tccagctcga
     4921 cgcgggcgaa aaatcccagg tgttcgagct ggcggcggtg gcacagaagc tcttggaccc
     4981 aggaaaagat ggcaagagca agcgcgagct gcggctggtt gcgctgggat cggcggacgc
     5041 gatgagcgac ggactggtgc cgtggctgcc gaaccgcgtg ctgatcatgg acagcgtcaa
     5101 gtggctgatg caggaagagg cgatcctcgg tgagccggta caggatcagg acgtaccgat
     5161 cgttcacacc aaaggccaag agaagctgtg gttctggctg acgacgctgc ttctgccttc
     5221 gtttgtgctg ggtgttggcc tgctctatac gcggtcggtt cgtcgtcgga ggtcttcatg
     5281 aagacagtga aaggacagca agggctttcg cccgtctatg tgcatgccgt gctggcgacg
     5341 ctcgcggtgg ggtttgcgta tcagacttgg acacgggacc ggacacagac tcagacggac
     5401 agcatcgtgg tgctcgacgt cgcaaaaaag gaagtgtcga gcctgacgta tgaagacgag
     5461 accaaacagg tcatcgttca gcgcaagacg ggcaccgatg gagagccgta tgcgtggctg
     5521 gtcacacgga ccaagtccaa ggtgccggtg aatccgcctg cgccacctac accgctggca
     5581 ccgcccgctg ccgtcgcgcc tgcaccgact gcggctcctg cggtgccgat caaggctggg
     5641 gcattcccga cggccaaacc cgcggctccg cccgtcaaag gtggcgacaa gagcggcgag
     5701 cctgcggttc ctccgggtca tccgccgctt ccgttggctg ggcctgctcc tgctgctgcc
     5761 ccgacttcgg tggctccggc agtggctgcg ccggcaccga ctccgccgcc cgtcgagatg
     5821 aaggaaatca cgacggtgaa ggagtaccgc ggcaacgacg gcgctgtgga actgctgggt
     5881 ttgtttggtc cgctcaaagc gctgcgcacg ctgggggctg tcgacgaagc caaagcgaaa
     5941 gagctgggct tttccggcag caaaaagaca ctcaccgtga gtgccaaggg gctgtcgtat
     6001 gcctttgtgc tgggtgggac gagctatggc agcggcgatt attacgtccg cgacccccaa
     6061 ggtcaggtgt atttgctgtc gcagcggatc attgcggact tcgagttttc agactcccgc
     6121 ttgatggagc gtcgactgca ccgattcgag cgcgccgata tcgatcgcgt cgaagtgacc
     6181 gttggcacca agacgcggac gtttgtccaa ggcagccggc aaaacccgca ggcattcttt
     6241 tttgccgagc aagcgactcc cgagaagcgc gacgactcga tcaaaaactg gatggaaaag
     6301 ctgctgcgac tggcgatcag cgactacgtg gcccagggcg aagatccatc gacaggcgcg
     6361 ggtgcttcgt cgatatctgg tgcggccaaa gttggcgacg tcatgacggc tcgttttttc
     6421 gacggacgca aggaaatcgg gcaggtgatg ctgtcgcgtt accccaacgc gaaaacgaac
     6481 cagcctgagt actacgctcg cacagagacg accatcggtc aggtccatct tctggctgcc
     6541 accgccgaga gcgtcgttca agacgccgag aagtggtagt gattcacttg ctctttcgtc
     6601 ccccactttc aaaagaaagt cgtcgcgcat gaacgtcctg tttgaagacg ggggcgtgct
     6661 gcgtgccgcg gcccttttgt cggaacaagc tggcgcgatc caggtggagt tccccggcgg
     6721 caaacgcgaa aaagtgcgtg ctgaccgggt gcttttgcac ttcgccaaac cgctgccgct
     6781 ggaactgctc accgacggac agaaactggc gacggaggtc gaccgcgagt ttctgtggga
     6841 agtcgcaggg gacgcagagt ttgctttttc ggatttggcg cgggagtatt tcgggcatcc
     6901 accgacggca attgaatcgg cggcgatgct gaccgtgctg tatggttcgc cgtcgcattt
     6961 ttcccgccgt ggggtaggtc gctttcaagc cgtgacgatg gaagcgcgtc aagcggcgga
     7021 aaaggcggcg gcgcgcaagc agaagcagct cgaaaagcag acggcgctgg ccgatcagct
     7081 catcgcggga gagctgccaa gagacatcgc ggctcggatg ccgcaggtgc tctacaaacc
     7141 ggacccaagc tggattgaaa ccaaggcgat ccttcaggcg tgcgacgcga cgcacatgtc
     7201 gccggccaag ctgctgattc gctgtggggc ggtcgacgga agtcatagct ttcatttggg
     7261 gcgttttctt ctcgattatt tccccaaagg gcgctcgatc gagacgacgg cgacggttga
     7321 tccgctgccg gacttgccgc tcgcggatgt ggcggcgttt agcatcgacg atacctcgac
     7381 gacggaaatc gacgatgcct tcagcgtgac aagacagcgt gatggctcgg ttcgcgtcgg
     7441 aattcacatt gcggcgccag cgctagggtt tcagccgggg tcggcactgg atgaactggc
     7501 acggtcgcgg ctgtcgacgg tgtacatgcc gggcgacaag atcacgatgc tgccgccgca
     7561 agcgataaag cccttcacgc tccttgaagg acacgactgt ccggcggtgt cgctgtatct
     7621 gacggtctct ggagacggtt cgtaccgaat tttgggcgaa gagagtcgac tggagcgggt
     7681 gccgattcgg cgaaacctgc gctatccggc gattgacagc cagttttccg tcgaaaacat
     7741 cgcgactggg cggcttgatt tcgagtttgg gcccgagctt ttgtttttgt acgagctggc
     7801 gggccggctg caagccagtc gtggcaagcc cgagcaagag caggtcgact acatcttttc
     7861 gtttgtcgac gggaaaattg cactgacgcg gcggatgcgt ggcggtccgc ccgacaaagt
     7921 cgtcgcagag ctgatgattt acgtaaactc ggcgtgggga aagctgctcg gcgatcgtga
     7981 catccgcgcg ctgtatcgcg tccaagacag tggcaaggtc cgtctgtcgc tgtatccggc
     8041 gccacacgcg ggacttggcg ttacgcagta cctgtggtca agctcgccgc tgcgtcgcta
     8101 ctgcgacttg gtaaaccagt ggattttgat cgcgcggctg tacgaagaac cgtcgccgta
     8161 tgccgacaat gaaggggaac tgctgaagct gatgcgtgac ttcgagcaga cctacggcgc
     8221 ttacgacgag ttccagcgca gcatggaacg ctattacggc atccggtatc tggcccaaga
     8281 aggcatcacg cagacaacag cgacggtgat tcgcgacaat ctggtgcggt ttgacttggt
     8341 gccgatttgg cagcgggtgc cgtctttgcc agagcttcca gcgggcacca gcgtcgttgt
     8401 cgatgtcgct cacctggatg agctggagct gacgctgcac gccgaattcc gacggaaatc
     8461 tgacggcgca gtttcgtgaa tttacgggcc ttcgcgcttg ccgtaatcga cgcggacgag
     8521 caaaagccca caagcgggcg cggtctgccc agcgcgcgtt cgatcgccgg tttttagcag
     8581 ggtggcaatc tcctctgtcg atcgcaggcc acgcccggca gcgacgatgg tgccgctcaa
     8641 gattcggacc atgtttttca ggaaagcggt gccctcgact tcgatgtaaa tggccgcggg
     8701 atcgtgtggc ggtaccagca cgtcgatgcg tttcatggtg cggacggtgg tttcgcgctc
     8761 acaatcggcg gcacgaaagg cccggaagtc atggcgtccg acgagaactt ccgcagcggc
     8821 gcgcatcttt tccagatcga gtggaaaacc aacgtgccag tggttgcgac agtgcagcgg
     8881 cgatcgaatg ttgccgttcc agatttgata gcgatagagc ttgccgctcg atgagtgccg
     8941 cgcatcgaac gaatcgtcga cgagttgagc agacaccacc gccaccgaat cgggcagatg
     9001 gttgtttgtg ccatagacca gcgctttttc cggaatctct cgtggcagcc gaatcgaaac
     9061 cacctggccc agtgcatgca cgcccgagtc cgtccgaccg gcagcgcgac agatcaccgg
     9121 ctctttgagg actttgccca gcgcttcttc aagcgcagcc tgcaccgacg gtccgttaag
     9181 ctgcctttgc cagccacaaa aatcggttcc gtcgtaacaa atgtggagtc gataagtagg
     9241 catggccgcg tccgcaggct ggcgtttatg acgaaacacg cttccgcttg gcaagacctt
     9301 gacggcgtag cgtgggctca ctatccttcg ccgctctcgg gccgatgcgc taatatcttc
     9361 ggccaaccgg gggccttccc ccctgtcgat acggaggaaa actatggctg ttcgcattgg
     9421 catcaacggc tttggtcgca tcggtcgctg tgtggttcgc gcttggcaaa aagacgccgc
     9481 caaggcgggc tacgaaatcg tcgccatcaa cgatctgacg gacaccccga cgctggctca
     9541 cctgctcaaa tacgactcgg tccacgggcg ttttgacggc aacgtcacgg tcgacggaaa
     9601 caacatcgtt gtcgacggaa aaccgatcgt agtcaccgcg gaaaaggatc tcaccaagct
     9661 cgactggcgc aagcacggtg tggagatcgt cctcgagtgc actggcgtct tcaccgacgc
     9721 accaaaagcg cgcacccaca tcgacgtcgg cggcgccaaa aaagtgctga tctcggcccc
     9781 ggccaagggt ccggacatca cgatcgcgct cggcatcaac caagaccagt atcagcacga
     9841 caagcatcac gtcatctcga acgcttcgtg caccaccaac tgcctcgctc cggttgccaa
     9901 ggttctcctc gacaacttcg gcatcgtgag cggcatgatg acgacgatcc acagctacac
     9961 aaacgaccag cgcatcctgg acctgccgca caaagacctg cgacgggccc gtgcggcggc
    10021 gctgtcgatg atcccgacta cgacgggagc cgcgaaagcg ctgtcggaag tgattccggc
    10081 gctcaaaggc aagctcgacg gatacgcgat gcgcgtgccg acgccgaacg tctcggtggt
    10141 tgacttcacc gtgcgcctgg aaaaaccagc gacggtggaa cagatcaatg cggcgtttcg
    10201 cgctgcggcg gaaaatccgg cgttccgagg cgtgcttgcg gtcagcagtg aacccacggt
    10261 gtcgtgcgac tacaacggcg atccacactc ggcaacggtg gacctcggat cgacgcagat
    10321 ggtcggcgac atggccaagg tgttggcttg gtacgacaac gaaatgggct actcgaaccg
    10381 gctgtatgag ctggcgaagc tggtttcgga aaagtcgcag tcggcctaac ttcgcgagga
    10441 gcagatcatg gcgaccattc gcaaggtgtc cgaactggac gtgacgggca agcgggtctt
    10501 tgtccgcgtt gatttcaacg tgccgcagaa cgacaagggc gaaatcaccg acgatacccg
    10561 cattcgtgcg gcactgccga cgattcgcaa cctcgtcgaa cgcggcgcgc gggtgatcct
    10621 gggatcgcac ctgggtcgac cgaaaggcaa gacgcccagt ctgaccatgc ttcccgttgg
    10681 acagcgtctt gcggagctgc tggcgccaca ggtcaaagag atcaagctct gcgacgaagt
    10741 ggttggcgac ggtgttcgca aggtgattgc tgatctgcgc gacggggaaa ttgcgctact
    10801 tgaaaacctg cgctgggagc caggcgaaga aaagaacgac gagacactgt cgaaagcgct
    10861 cgcctcactt gccgaggtgt acgttaacga cgcttttggg acggctcatc gggcgcactg
    10921 ctcgacggcg ggtatggtca agcactttgc ccccggaaag cgcggcgcgg gctttttgat
    10981 gctgcgcgag gtcgagtttc tgtctcggct tttgcacaac gtcgagcgtc cgtatgtcgt
    11041 cgtgatcggc ggcgccaaag tgtcggacaa gatcaacgtc ctcaccaact tggcgagctg
    11101 cgcgagcagc gtgctgattg gcggcgcgat ggcgaacacc ttcctcgctg cggagggcaa
    11161 aaacctcggc aagtccaagc tggaagcgga caagctgcaa gtcgccaaac agttcctcga
    11221 tcgctgtgcg cgcgacgggg tggatatcgt gctgccgacc gatctggtgg tcgctccgtc
    11281 gattgatgcg gctgaaggaa ccgttgtcgc ggtcgatgcg gtgccgagtg atcaaatggc
    11341 gctcgacatc ggtccggcat cggcacgcga gtttgcgagc ctgatccgcg aggccaagac
    11401 ggtgttctgg aatggaccgc tagggctgtt cgaaaagaag ccgtttgccg ccggaacgtt
    11461 tgcgatcgcg cgggcgctgg cggaatgtgc gggcacgacg gtggttgggg gcggcgattc
    11521 cgtcgcggcg gtttcagatg ccggcgtcga atcgaaagtc agccatgtct cgacgggcgg
    11581 cggcgcatcg ttggaattcg tcgaaggcca gacgctgccg ggcatcgctg cactttatga
    11641 accggagagc gcgcaatgag ccgctcagct ctcatcgtcg gcaactggaa aatgtacaag
    11701 accgttgctg aaggtcttga actggcgacc gcagtcaaaa atgcggttgc ccccacggtc
    11761 cgtggtcgcg aagtcgccgt tgcgattgcg ccaccgtttc cctcgctgta tccgattggc
    11821 aaacgactcg aaggctcgac ggtggccctg gccgcgcagg actgcttttg ggaaaatcag
    11881 ggtgcgttca cgggcgaagt ctcagcggcg atgcttgccg atgtgggctg caagttcgtg
    11941 atcattggtc actcggaacg ccgtcagcac tttggcgaga cggatgcagc ggtcaatcta
    12001 aaagccaaag cggtgctaaa agccggtctg ttgccgattg tctgcgtcgg agagaccgaa
    12061 aaagaacgcg acgccggcga aatgagaggc cggatcatcg cgcagcttgc cgaaggactg
    12121 ttcggtttca cgcccgacat gatgcagcgg acggtgattg cctacgaacc ggtctgggcg
    12181 attggtacgg gtcgggtggc gacgaccgcg caggcccagg aagtccacgc gcttatccgt
    12241 cagtatctgc acgcacagct tggcgaagtg gcgggctctg tacgaatctt gtacggtggc
    12301 tcggtaaaac caaataatat cgccgggtta atggcgcagc ccgatgtcga tggcgcactc
    12361 gtcggcggtg cttcccttcg ggcagaagaa ttcttgcaga tcgttcgctt tcacgaggct
    12421 ccagacgcaa catgatgacc ttgctctaca ccatccacgt cctcgtctgc ctgttcctga
    12481 tgctggtgat tttgctccag gcaggcaaag gcggaggcat gggcgctctt gggtcggggg
    12541 cgacccagca agtctttggc ggtcgaggcg ccagcaattt cctcgagcgc ttgacgaccg
    12601 cgacggcagt tgtgttcatg ctgacggcgg tcacgctgtc gtacatcaac agccagtcag
    12661 aatcggacaa gctggcgaag ctatcggcca agaaaaaatc cgacgaaaag agcgcgaaga
    12721 aaaaagccga agattcgctg aagcagcagg ttgaaaagcg ggctgcggaa gaaaaagcgg
    12781 cggagcagaa ggcgctggaa gaaaagctga aagccgcggc tgctgcggcg gctgctgcca
    12841 cttcggatgg tggtgcggat gcttcgacgg gagctgcggt gcctggtgtc ccgagtgcgg
    12901 ctccgactgc tccgggcgcg gcgcctgctg cccagccggg ggctcccaag ctcacggtaa
    12961 cggcgactcc tcctgcggct ccgactgcag ccaagcccgc aactggtgtt ccagccccgg
    13021 ttgcgaaccc a
//
LOCUS       DAORGN010000143        12942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2004_length_12942_cov_5.071467, whole genome shotgun sequence.
ACCESSION   DAORGN010000143 DAORGN010000000
VERSION     DAORGN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_12942_cov_5.071467"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PKL17_14880"
     CDS             <1..666
                     /locus_tag="PKL17_14880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNK46067.1"
                     /translation="GVALTVEYDPVVLALEDENSDEESAKTVSAEAVVTDAAAQPAHL
                     SPKTVVDIGTGSGAIAISIKHERPSVRMLAIDRSRAALDVAEQNAAKLAVAIEFFEGD
                     LLAALPDGQRVDLILANLPYIPTADIAQLDKDVQSEPKLALDGGPDGLVLIRRLCAMA
                     PARLSPGGSILLEVGQGQAPAVEQILADRGFVNVRSLLDLAKIARIVCGKLPTQTEGV
                     SHD"
     gene            659..1360
                     /locus_tag="PKL17_14885"
     CDS             659..1360
                     /locus_tag="PKL17_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46068.1"
                     /translation="MTELPVGWFLSLLLGARHALEPDHVAAVTTMLTTERGRPAALGV
                     LWGAGHALALFGMATTLSLLERQLSPEWADRCELLVGGMLIFLGGRAVILVLRALHEG
                     RQGLLHTHSHGGARHVHSGPLGHVHIGGYTFAHRPLLVGLFHGLAGSGALTALVSTKM
                     PGIWDRLAYVSLFGIGSMLGMGALAGLLGWPLSRWLAARPLVGQLLTVTAGILSMGLG
                     VLWSLPIVDRLWSGS"
     gene            1371..2597
                     /locus_tag="PKL17_14890"
     CDS             1371..2597
                     /locus_tag="PKL17_14890"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46069.1"
                     /translation="MKARITHLFPLLLGLLWPSLCQADVATKNLPPAVYAAPLGEPLP
                     PSLAESLGPTVRFIEPGPVRQAAAQVVEARAAIASLRCSEVVPRLRTAGDALLGEVPI
                     QNGMAVARDLFGLLLLCADRIGDVPSAERAARFLATQKDPLPADLALILPRYQQSGIF
                     GPPRAPVKVESDPSGATVMRNDQVMGTTPLTIDGGRPDVDFLTLELPGMRKLRRPLGS
                     GENLFLSLRPEDRLPLLFDQVAGLPIGSEQQTAVLKELAGSPAVATQVGTRLLVFGPK
                     LRDGKPVVGESLVARVFDFARKDWLANQTEIPSGNAASQAAALAAVLSTNGPAPAAQT
                     VTAAATPAGVQPVTATNAKGTAPAPAEKSSGSKLPFAKTKWYTWVVAGGVAALIGGLL
                     IAERFSKDKLTVTATH"
     gene            complement(2648..4555)
                     /locus_tag="PKL17_14895"
     CDS             complement(2648..4555)
                     /locus_tag="PKL17_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46070.1"
                     /translation="MNRLTITRGFLALLVTVPTLAFAQQETPTGYPQYGYPQPTYPQP
                     TYPQPTQYGNPNQPYPGAQPYPGGQPNPGAQPYPNQPYPNQPYPGAQPYPNQPYPGSP
                     PSATGYPNPAPYPGQADPQRNPASSAQTATETKLVLTASSEEAKNALSACLDAMDHFR
                     QDVVRQRCSEAIEKDDKLALAHALLAQSSGQPLVQKRHLTAAKEITRPVSEGERLLIE
                     GMIALAEDQRQVARAAFEAMAALLPADKHAHYYRGLLRYRFGDLDGAQTDLKKTLELD
                     GKFGPAHNALGHLALRRDNVEEAAKEFAKYVELHPKEPNAHDSHAMLLLRKGELGAAV
                     EAARKSLEVDAKFLRGNLRLGDALLLQGNPLLARKAYSAVLSSPDPSEHHEAALRLSR
                     SRLFEGLGLPTPKALQEAEKDLLTELELSKKLSRRADQVQTLLELARLQFERGAQSDA
                     VKTIGQIRDLLPSDQAATDDKNDSAKPTDKALADKAAPTLTDEEKSRFAADLLWLRAL
                     LLYSIGERDLAKERADDIEKTLRGKPGQRSAEDLRGELAARSGDRQKVVEHLSQSTRP
                     TGRLALALALGGGKPGEQIDMPKARTMLEELGRRNVIDLEGALTRGRAKQWLRQNPAE
                     KVDGKSSETKP"
     gene            complement(4615..6288)
                     /locus_tag="PKL17_14900"
     CDS             complement(4615..6288)
                     /locus_tag="PKL17_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46071.1"
                     /translation="MKQLAEVQDPYIGMLLNDRYKIIKKLGAGGMGAVYLAEHVMIER
                     RVAIKILSQDFASKPDLVQRFIQEARAAARIGHENIVEVHDFGETASGSVFFTMEFLE
                     GHDLAHAIQEAGSLPIPRIRHIMGQLCRALAAAHAKGIVHRDLKPENIYLVDKEGKSD
                     FVKILDFGIAKMTTMEAGNAKPGGGAGTGNRLTQSGMIFGTAEYMSPEQARGETPDHR
                     VDIYALGCILYEMLTGKVPFHAETYMGTLTKHMFEAPEPPSRRNRNIPTDIEAICLRA
                     MDKDRARRFQSMTEFAQAIDGELAVTPPPPVQDPSMSMRSAGFPPSVIPQGMGMGAPG
                     YAPPMMMQPGPYGVPGQVQAPMGLHQSGIISAPLAMSPSGMVSAQMAIGPSGMIANPM
                     VSGIHMHAGGFTQTAPPAYEVARPPAGGQLRRLVFIFAGAAIGVAVLALLLLWPQAGG
                     PGPVPAVANNPGVSAAQRPKGPARLTIRGLPGSKVSVDFVEIGTIPGGGEIQHEISSA
                     TGAPEWSVSVRVVKTGFDEFVTRQTVRAGQNFTLQASQSSLGAGNKSPK"
     gene            complement(6477..8210)
                     /locus_tag="PKL17_14905"
     CDS             complement(6477..8210)
                     /locus_tag="PKL17_14905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46072.1"
                     /translation="MKTCPQCQIKYPDVLEFCPRDGSPLPSTSGPKEVEADPLLGTTI
                     DGRYAIEARLGEGGMGVVYKARHVLIDKPVAIKILRKEAAQDTAAVQRFIQEAKSASK
                     IGHSNIVDITDFGVLPDGHAYFVMEFLQGQTLAQAILEGPLDPARVCLIAAQMARGLN
                     AAHTKGIVHRDLKPENIFLLEREGQKDFVKIVDFGIAKVGSGQKLTQVGMVLGTPEYM
                     SPEQATGQETDHRVDQYATGCIMYEMLTGVVPFLGERPAQTLTKHVFEPIIPPRKRKP
                     ELKIPGSLDAVVIRAMAKKPEARFPSMKELEQALTQVEQEMKGGRIVIDDPGMAAPQA
                     PPPVAPVAQPPVMPGYPPQPGFQQPMAPAMYAQPMAGFPPMVAPTPPRPTGKSQTVDS
                     KGVKPVLIGVAIVLVAVIGLLLYFLLFQEQPKPVVPPPPLPKPLVKPSEPITPPPPPK
                     AEEAGPVYLMITTKPPGAEVVVDGDVRGITPLRIAHKKGQPLHLELRRKGFESYSEDM
                     LPEKDQDVDVPLEKTGGGRSKSGDAKKSGSKPSAPKNDAGKKNGGKTGSPEKSGGSLP
                     TGLRDPFSRGK"
     gene            complement(8345..9754)
                     /locus_tag="PKL17_14910"
     CDS             complement(8345..9754)
                     /locus_tag="PKL17_14910"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNK46073.1"
                     /translation="MALLLLGANCRRETVTTPATASADAGTTRPDAESPAHAATSKSS
                     DHAKTKPPEPLQRCFPEDSALSPPRSIAALLDRAADRLEQASEQPRVCTGKDKEPDPP
                     RLLRDALACTEEALRKDEQSVEAHHNRGLALLGLDRKDEAQRAFTHALAIDPDDPETL
                     AAAAELYIHHLSPSTENTLIGLAYVRHGKAVLRRANAKPDRRTTIAVAASPTKRGQAS
                     GPKASKATRSERPDRTPRPSSERQRSLLGRLLLLEGQALSDLGRARLALGVLDEAITL
                     TDSDQARYERGLVLFDLCRLKEAQTTFSDLTRRMPDDAWSHHQLGLVLEMLGDGNAEK
                     ELATARKLAPADFPEPLPVSKSQFQKMVAEEVTALSPTDRDDLRKVRLELTDLPAIED
                     LTVDEPTLSPTIVGLYRGLPAGVPDSEPRSIVLYRKNLLRIVSTQEELRDQVRTTLLH
                     ELGHLRGEDDDELRDRGLE"
     gene            9925..11106
                     /locus_tag="PKL17_14915"
     CDS             9925..11106
                     /locus_tag="PKL17_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK46074.1"
                     /translation="MLLRFFFRLREVGLPTSLTEYLTLLEALKQRVVSLSVDDFYFLS
                     RSCLVKDERHLDRFDRVFGETFSGLESLADPQTGAAIPEEWLRKLAEKLLSPEEMAQI
                     QALGGFDKLMERLAELLKEQHERHQGGNRFIGTAGTSPFGAYGYNPEGVRIGQDRSRH
                     RKAVKVWDKREFKNLDDNIELGIRNIQVGLRRLRRFARQGAATELDLPGTISKTAENA
                     GFLDLQMVPERHNTVKVLLLLDIGGSMDDHVQSCAELFSAARTEFKHLMHYYFHNCVY
                     ESLWKDNARRSTERTPTHDVMHTYPDDYKLIFVGDAAMSPYEIVQRGGSVEHWNEESG
                     AVWMQRLLDTYPHAVWLNPLPEAAWQHYQSVEMVRHLMHGRMYPLTLHGLDDAIRELS
                     H"
     gene            complement(11110..11628)
                     /locus_tag="PKL17_14920"
     CDS             complement(11110..11628)
                     /locus_tag="PKL17_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46075.1"
                     /translation="MKRAMLCTAFALAALLISACDSPPKPIPMGRCATEQECTPAEGT
                     CLSPGQFAGCGICRRPLPSEICTSDAACAATGPTAICTNTPALCLCGGETVCTNGCAA
                     DSECSEGQRCAANHRCEPKACVSASDCPTDFRCDAGKCSRKPCATSRDCSGYCVNSAC
                     YSSPGTCNLPRP"
     gene            11935..>12942
                     /locus_tag="PKL17_14925"
     CDS             11935..>12942
                     /locus_tag="PKL17_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNK46076.1"
                     /translation="MENTSDLVKVSLAALGVVYGDIGTSPLYAFKECFTGEHGVPVDP
                     ANVFGILSLIFWSLTLVVVFKYMTFIMRADNHGEGGILSLLAVLAPRGNVDKRAIGRL
                     SLVLFGLFGSALLYGDGIITPVISVFSAVEGVQVYAPMIGTGKFAVSTASLVMPLTAS
                     ILVLLFLMQKRGTAGVGALFGPVMMVWFFAIALFGIVGVIRNPSVLWAMNPIHAIRFF
                     VQHKMHGFLALGAVVLCVTGGEALYADMGHFGQRSIRLAWYTLVFPSLLLNYFGQGAY
                     LLSHGKPEGTFNPFYQIVPSWAQAPMVVVATLATIVASQALISGGFSLTRQAVQLGYW
                     PR"
BASE COUNT         2153 a         3790 c         4195 g         2804 t
ORIGIN      
        1 ggtgtggcac tgaccgtcga atatgatcca gtcgtgctgg cattggaaga tgaaaacagc
       61 gacgaggagt ccgcaaaaac ggtgtccgca gaagcggtcg tgaccgatgc tgcggcgcaa
      121 ccggcgcacc tttccccgaa aaccgtcgtc gacatcggca ctggcagcgg ggcgattgcg
      181 atttcgataa agcatgagcg tcccagcgtg cgcatgctgg cgatcgatcg cagtcgtgcg
      241 gctctcgatg tggccgagca gaacgccgcg aaactggcag tggcgatcga attttttgaa
      301 ggcgatctac ttgcggcact tcccgacgga cagcgtgtgg atctgatcct ggcgaatctt
      361 ccgtatattc cgacggcgga catagcgcag ctcgacaaag acgtgcagag cgaaccgaag
      421 ctggcgctcg acggtggacc tgatggactt gtgctgatcc ggcggctttg tgcgatggct
      481 ccggcgcggc tatcccccgg cggcagcatc ttgctggaag ttggccaagg acaggcgcct
      541 gccgtcgaac agatcctggc agaccgaggc tttgtcaacg tgcggagctt gcttgacttg
      601 gcgaaaatcg cgcgaatcgt gtgcgggaag cttccgacgc agacagaggg tgtttcccat
      661 gactgagctt ccagttggct ggtttttgtc gctgcttttg ggagcccgtc acgcgctgga
      721 accggatcac gtcgctgcgg tcacgacgat gctgacgacg gagcggggcc gaccggctgc
      781 gctgggcgtg ctgtgggggg ccgggcatgc gctggcgctg tttggcatgg caacgacgct
      841 ttcgctgctc gaaaggcagc tttcgccgga atgggccgat cggtgtgaac tgctcgtcgg
      901 tggaatgttg attttcctgg gcggacgggc ggtgatcttg gtgctgcgtg cgcttcacga
      961 ggggcgacaa gggcttttgc acacgcactc gcacggcggg gctcgtcacg ttcacagcgg
     1021 accgctcggg cacgttcaca tcggcggtta cacgtttgcg catcggcccc tgctcgtcgg
     1081 gctctttcat ggtctggccg gttcgggcgc gctgactgcg cttgtgtcga cgaagatgcc
     1141 gggcatttgg gatcgtctgg cgtatgtttc gctgtttggg attggctcga tgctgggaat
     1201 gggcgcgctg gcggggctct tgggctggcc gctgtcgcgc tggctggctg cccgtccttt
     1261 ggttggtcag cttctcaccg tgacggcagg cattctgtcg atgggtcttg gggtgctgtg
     1321 gagtcttccg atcgtcgacc ggctgtggtc cggtagttag gatgccgagc atgaaggcgc
     1381 ggattacaca cttgtttcca cttctgctag ggctgctgtg gccgtctttg tgccaagccg
     1441 acgtggcgac gaaaaacttg ccgccagcgg tctacgcggc gccgctcggt gaacccctgc
     1501 caccgtcgct cgccgaatcg cttggtccga ccgtgcgctt catcgagcca ggaccggttc
     1561 gccaggcggc tgcccaggtc gtcgaggcgc gggccgccat cgccagtctg cgctgctcgg
     1621 aagtcgtgcc caggctgcgc acggcgggcg atgcgctgct tggtgaagtg ccgatccaga
     1681 acggcatggc cgtggcccgt gatctgtttg gacttttgct gttgtgtgcc gaccggattg
     1741 gcgatgtgcc gtcagcagaa cgtgcggccc ggtttttggc gacccagaaa gatcctctgc
     1801 ctgccgattt ggcgctgatt ctgccgcgct atcagcagag cggtatcttt ggtccgccgc
     1861 gggcgccggt gaaggtcgaa agcgatccgt cgggagcgac ggtgatgcgc aacgaccaag
     1921 taatggggac gacgccgctg accatcgatg gtggccgtcc cgatgtcgat tttctgacgc
     1981 tggaactgcc tggaatgcgc aagctgcgtc gtccactcgg cagtggcgaa aacctgtttt
     2041 tgtccctgcg tcccgaggat cggctgccgc ttctgttcga tcaggtcgcg ggactgccca
     2101 tcggcagcga gcagcagacc gcagtgctca aggaactggc cggatcaccg gcggtggcga
     2161 cgcaagtcgg gacgcggctg ctggtctttg ggcccaagct gcgcgatggc aaaccggtgg
     2221 tcggcgaatc gcttgtggca cgggtgttcg actttgcccg gaaagactgg ctggccaatc
     2281 aaaccgaaat cccgagcgga aatgctgcat cccaggccgc cgcgctggct gcggtgctgt
     2341 cgacaaacgg gccagcgcca gccgcgcaaa ccgtcaccgc tgcggccacg cctgctggtg
     2401 ttcaacctgt gacggcgacg aatgcaaaag ggacagcccc agccccggcg gaaaagtcat
     2461 ctggatcgaa gctgcctttt gcgaagacca agtggtacac ctgggtggtg gcgggcggcg
     2521 tggcggcgct gattggtggc cttcttatcg cggagcgttt ttccaaagac aagctcaccg
     2581 tgacggccac gcactgattc gcaggccgac ccggcgcaaa tgcagagcgg gcgaaatccg
     2641 gcagttttta cggcttggtt tcgctcgatt tgccgtcgac tttttcggct gggttttgcc
     2701 gaagccactg ctttgcgcga cctcgtgtga gcgcgccttc caggtcgatc acgttgcgac
     2761 ggccaagctc ttccaacatg gtgcgggctt tgggcatgtc gatctgctcg cccggcttgc
     2821 caccaccgag cgcgagcgcc agcgccagcc gtcccgtggg tcgtgtgctc tgcgacagat
     2881 gctcgacgac tttttgacgg tcgccactgc gcgcggccag ttccccgcgc aggtcttcgg
     2941 cgctgcgctg tcccggtttg ccgcgcagcg ttttttcgat gtcatcagcg cgttctttgg
     3001 ccaggtcgcg ctcgccgatc gagtacaaaa gcagcgcccg cagccacagc aagtcggcgg
     3061 caaagcgtga cttttcctcg tcagtcagcg tcggtgccgc tttgtccgcg agcgctttgt
     3121 cggtcggttt tgcggaatcg tttttgtcgt ccgtcgccgc ctggtcgctc ggcagcagat
     3181 cgcggatctg accgatggtc ttgaccgcgt cgctttgtgc gccgcgctcg aactgaagcc
     3241 gcgccagctc cagcagcgtc tgaacctgat cggcacgtcg cgagagcttt ttcgacagct
     3301 ccagctccgt tagcagatcc ttttctgctt cctgtaacgc tttgggcgtc ggcagcccca
     3361 gtccttcaaa caaccgactt cgcgacagcc gcagcgctgc ttcgtggtgc tcgctcgggt
     3421 cgggagacga caaaaccgcc gaataggctt tgcgcgccaa aagtggattg ccctgcaaaa
     3481 gcagcgcatc gccaagccgc aggtttccgc gcaaaaactt ggcgtcgact tcgagcgact
     3541 tgcgggcggc ctcaaccgcg gcacccagct cgcctttgcg gagcagcagc atcgcatgcg
     3601 agtcgtgcgc attcggttct ttggggtgaa gctcgacgta tttggcaaac tctttggccg
     3661 cttcctcgac gttgtcgcgg cgcagcgcca agtgccccag cgcgttgtgc gccggaccaa
     3721 atttgccatc cagctccagc gtttttttca gatccgtctg tgccccgtcg agatcgccaa
     3781 accgatagcg aagcagtccg cggtagtagt gcgcgtgctt gtccgccggg agcagcgcgg
     3841 ccatcgcttc aaacgccgcc cgtgcgactt gtcgctgatc ttcggccagc gcaatcatcc
     3901 cttcgatgag caagcgctcg ccttcactga cggggcgcgt gatctctttg gccgccgtga
     3961 gatgtcgctt ttgcacaagt ggctgaccgc tcgactgcgc caggagcgca tgggccagcg
     4021 ccagcttgtc gtctttttcg atggcctcgc tgcagcgctg gcggacgaca tcctgtcgga
     4081 agtgatccat cgcatccagg caggccgaga gcgcgttttt ggcctcttcg cttgaggcgg
     4141 tcaggaccag cttcgtttcg gtggcggttt gcgctgaact ggctgggttc cgctgcggat
     4201 cggcttggcc ggggtacggc gccggattcg gatagccggt tgcactcggt ggactgcccg
     4261 gataaggctg gttcgggtaa ggctgagcgc ccggataagg ctggttcggg taaggctggt
     4321 tcgggtaagg ctgagcgccc ggattcggtt ggccgcccgg ataaggctga gcacccggat
     4381 aaggctggtt cgggttcccg tactgcgtcg gctgcggata ggttggctgc ggataggttg
     4441 gctgcggata gccgtactgc ggatagccgg tgggcgtttc ttgctgtgca aatgcaagcg
     4501 tgggcaccgt cactagcagc gccaggaatc ccctggttat ggtcagtcga ttcatcggag
     4561 gcctcgctgc gctcgtttta ctacggcacc gcgtgtgtgt ccgcagcgag cgcgctattt
     4621 gggtgatttg ttgccagcgc ccagagagga ctggctggct tgcagcgtaa agttttgtcc
     4681 ggcgcgcacg gtctggcggg tgacaaactc atcgaagccg gtttttacga cgcgcacgga
     4741 tacggaccac tcgggggcgc ctgtggccga cgagatttcg tgctgaattt cgccgccgcc
     4801 cggaatggtg ccgatttcga caaaatcgac cgacactttt gatcctggca gaccccgaat
     4861 ggtaagccgc gccggtcctt tggggcgctg tgccgccgac acgccaggat tgttggcgac
     4921 cgcgggtaca ggtcccggcc cgcctgcctg tggccacagc aaaagcagcg cgagcaccgc
     4981 cacaccaatc gccgcaccgg caaagatgaa caccagccgc cgaagctgac cacccgcagg
     5041 tggacgcgcg acttcgtagg ccggaggcgc cgtctgggtg aatcccccag cgtgcatgtg
     5101 tatgccactg accatcgggt ttgcgatcat tcccgacgga ccaatcgcca tttgagcact
     5161 gaccataccc gacggactca tcgccaaagg agcgctgatg attccactct gatgcagtcc
     5221 catcggcgct tgcacttggc ccggtacgcc atatggaccc ggctgcatca tcataggtgg
     5281 tgcatatccg ggggcgccca tgcccattcc ttgcggaatc accgatggtg gaaagcccgc
     5341 cgagcgcatc gacatcgacg ggtcttgcac gggcggcggt ggggtgacgg ctagctcgcc
     5401 atcaatcgct tgggcaaact cggtcatcga ctgaaaacga cgggcgcgat ctttgtccat
     5461 cgcccgcaga cagatcgctt cgatgtcagt cgggatgttg cggttgcgac ggctcggcgg
     5521 ttccggtgcc tcgaacatgt gcttggtcag cgtgcccatg tacgtttcgg cgtggaatgg
     5581 cactttgccc gtcagcattt cgtacaggat gcagccgagc gcgtagatgt cgacgcgatg
     5641 atcgggggtt tcgccacgcg cttgctcggg ggacatgtac tcggcggtgc cgaagatcat
     5701 gccgctctgc gtcagtcgat tgccggtgcc ggcgccacca ccgggcttgg cgttgccggc
     5761 ctccatcgtc gtcatcttcg caatgccgaa gtcgaggatc tttacgaagt cgcttttgcc
     5821 ctctttgtcg acgaggtaga tgttttccgg tttcaggtcg cggtggacga tgcccttggc
     5881 gtgtgccgct gcgagtgccc gacagagctg ccccatgatg tgacggatgc gcggaatcgg
     5941 cagcgacccg gcttcttgaa tggcgtgcgc cagatcgtgg ccttcgagaa actccatcgt
     6001 gaagaagacg gagccggaag cggtctcgcc gaagtcatga acctcgacga tgttttcgtg
     6061 accgatgcgg gctgcggcgc gggcttcctg aatgaagcgc tggaccagat cgggtttcga
     6121 tgcaaagtct tgcgacagga tcttgatggc gacccgtcgc tcgatcatca catgctctgc
     6181 caaatagacc gcacccatgc ccccagcacc gagctttttg atgatcttgt agcggtcgtt
     6241 gagcagcatc ccgatgtacg ggtcctgcac ctcggccagc tgtttcaaga cggctgcttc
     6301 gcgggcgtcg agcgagtgtc cgccaggtga cgaatccttc gcccctttcg tgcccggtgg
     6361 cgacgcagcg tcgtctggtt tgggtggatt tggcactgta gtgccaggtt atcacaaccg
     6421 gaatgccgga accatcgccg aagcggctcg gcgctgggcg gccaaagtga cgcggactac
     6481 ttcccgcgtg agaacggatc gcgaagaccg gtgggaagcg agccgccgga tttttcggga
     6541 gacccggtct ttccgccgtt ctttttgccc gcgtcatttt tgggtgcgga cggtttgctg
     6601 ccggattttt tggcgtcgcc ggacttgctg cgaccgccgc cggtcttttc gagcggcacg
     6661 tcgacgtcct ggtctttttc cggcagcatg tcctcactgt agctctcgaa gcccttccga
     6721 cgcagctcca gatgcagcgg ctgccctttt ttgtgggcaa tccgcagcgg ggtaatgccg
     6781 cgcacgtctc cgtcgaccac cacttctgct cccggcggct tggtggtgat catcaggtac
     6841 acgggcccgg cttcttccgc tttgggtggc ggcggcggcg taatcggttc gcttggcttc
     6901 accagcggct tgggcagtgg tggcggcggc acgaccggct ttggctgttc ctggaagagc
     6961 aggaagtaca gcagcaagcc gatgactgcg acgagcacaa tggcgacgcc gatgagcacc
     7021 ggcttgacgc ccttggaatc gacagtctgg ctcttgccgg tcgggcgcgg cggggtgggg
     7081 gccaccatcg gtgggaagcc cgccatcggc tgcgcataca tcgccggcgc catcggctgc
     7141 tggaaccccg gctgcggcgg atatcctggc atgaccggcg gctgggccac gggcgcgact
     7201 ggcggcggag cctgaggggc cgccatgcct ggatcgtcga tgacgatccg tccgcctttc
     7261 atctcttgct cgacctgggt gagcgcctgt tccagttcct tcatcgacgg gaatcgcgct
     7321 tctggctttt tggccattgc acgaatcacc acagcatcga gagaaccggg aattttcagc
     7381 tctggtttgc gcttgcgcgg cggaatgatc ggctcaaaga cgtgcttggt cagcgtctgc
     7441 gccggtcgtt cacccaaaaa tggcacaact cccgtcagca tctcgtacat gatgcagccg
     7501 gtggcgtact gatcgacgcg gtgatcggtc tcttggccgg tggcctgctc gggcgacatg
     7561 tactctggtg ttccgagcac catgccgacc tgggtgagtt tttgccccga gccgactttg
     7621 gcgatgccga agtcgacgat ctttacaaag tctttttgtc cctcgcgctc gaggagaaag
     7681 atgttttccg gtttgaggtc gcggtggacg atgcccttgg tgtgcgccgc gttcagaccg
     7741 cgtgccatct gtgctgcgat caaacagacg cgcgctgggt ccagcggtcc ttccaaaatc
     7801 gcttgcgcca gcgtctgtcc ctggagaaac tccatcacga aataggcgtg tccgtcggga
     7861 agaacgccga aatcggtgat gtcgacgatg ttgctgtggc cgatcttcga agcggacttg
     7921 gcttcttgga tgaagcgctg caccgccgcc gtgtcctgtg ctgcctcttt gcgcaggatt
     7981 tttatcgcca ccggcttgtc gatgagcaca tggcgggcct tgtagaccac gcccatgccg
     8041 ccttcaccca gccgcgcttc gatggcatat cgaccatcga ttgtggtgcc aagcagcggg
     8101 tctgcctcga cttctttggg gcccgaggtg ctgggcagag gagacccatc ccgagggcaa
     8161 aactcaagca cgtccgggta cttgatctga cactggggac aagtcttcac agccggctca
     8221 gcttaccttt cttcgctact tacggtcaat cgggggcttg cgatgtgccc tgccgttttc
     8281 gtggttttcg atggtgagaa tacaggtttt gctgttttta aggtcgccgg ttgcgtgagg
     8341 caggttactc caggccacgg tcgcgcagtt cgtcgtcgtc ttcaccgcgc aagtgaccta
     8401 gctcgtgcag cagggtggtt cgtacctgat cgcgcagctc ttcttgtgtc gatacaatcc
     8461 gcagcagatt ttttcgatac agcacaatcg accgtggctc actgtcgggc acacctgcgg
     8521 ggagtccgcg ataaaggcca acaatcgtcg gtgaaagcgt cggttcatcc accgtcaagt
     8581 cttcgatcgc tggcaggtcg gtcagctcca gccgcacttt gcgcaagtca tcgcgatccg
     8641 tcggtgaaag cgccgtaact tcctcggcga ccatcttttg gaactggctc ttgctgacgg
     8701 gcagtggctc gggaaaatcc gccggagcca gcttgcgggc cgtcgccagt tccttttcgg
     8761 cgttcccatc ccccagcatt tcaagcacca ggccaagctg atgatgcgac caggcgtcgt
     8821 cgggcattcg ccgggttaga tcggaaaatg tggtctgcgc ttctttgagc cgacacagat
     8881 cgaacaacac caggccgcgc tcatagcgtg cctgatcgct gtccgtcagg gtgatcgctt
     8941 cgtcgagcac accgagcgcc agccgtgccc gtccgaggtc tgacagcgcc tgtccttcca
     9001 gcaaaagcag ccgtcccaga agcgatcgct gcctctcgct gctcgggcgt ggcgttcgat
     9061 ccggtcgctc ggaacgggtg gctttgctcg ccttggggcc gctggcttgg ccccgcttgg
     9121 tcggggacgc agcgacggcg atggtggtgc ggcggtcggg tttggcgttc gcgcggcgca
     9181 gcactgcttt gccgtgacgg acgtaagcga gcccaatcag ggtattttcc gtgctcggcg
     9241 acaggtggtg gatgtaaagc tccgccgcag ctgccaaggt ttccgggtcg tcaggatcga
     9301 tggccagcgc atgggtgaat gcacgctggg cttcgtcttt gcgatcaagt ccaagcagag
     9361 caagtccgcg gttgtggtgc gcttcgacgg actgttcgtc ttttcgcagc gcttcttcgg
     9421 tgcaggccag tgcgtctcgc agcagacggg gcggatcggg ctctttgtct ttgccggtgc
     9481 agacgcgcgg ctgctcgctc gcttgttcga gccggtcagc ggcgcgatcc agcagtgccg
     9541 caatcgatcg tggcggcgac agcgccgagt cttcgggaaa acagcgctgg agcggctcgg
     9601 ggggtttggt tttcgcgtga tccgacgatt tgctcgtcgc tgcgtgggct gggctttcag
     9661 catcgggcct tgtcgtccct gcatcagcag aagcagtcgc cggagtggtg acggtttctc
     9721 gacggcaatt cgcacccagc agcagcaaag ccacgagcgc gacagaacga caggaaaaag
     9781 agacggtacg caacgtcgcc attattgcaa aaagttcggc agcgacaacc ctcgcgacgg
     9841 aaaaagccgc aaacggcaag tcgtttggtc gcatgcgaaa gccgtttggc ttgagccccg
     9901 ccaaaggtcg cgcataatcc gcacatgctc ctgcgctttt ttttccgact gcgagaagtc
     9961 ggtttgccga cgtcgctgac ggaatatttg accttgctcg aggcgctcaa gcagcgggtg
    10021 gtttcgctga gtgtcgatga tttctacttt ttgtcccgat cgtgtctggt caaagacgag
    10081 cggcacctgg atcgtttcga tcgcgtgttt ggcgagacgt tttcggggtt ggaatcgctt
    10141 gccgatccgc agacgggcgc cgcgataccg gaagagtggc tgcgcaagct ggcggaaaag
    10201 ctgctttctc ccgaagaaat ggcgcagatt caagcgcttg ggggctttga caagctgatg
    10261 gagcggctcg ccgagctgct caaagaacag cacgagcgac accaaggcgg aaatcggttt
    10321 atcggtacgg cgggcacgtc gccgtttggc gcctatggct ataaccccga gggtgtgcgg
    10381 attggtcaag atcgctcgcg ccatcgcaaa gcggtcaagg tctgggacaa gcgagagttc
    10441 aaaaacctcg acgacaacat cgagctggga atccgcaaca ttcaggtcgg tctgcggcgg
    10501 ctgcggcgtt ttgcccggca aggcgcagcg acggagcttg atctgccggg gacgatctct
    10561 aaaacggcgg aaaatgccgg ttttctcgat ctgcagatgg tgcccgagcg gcacaacacg
    10621 gtgaaagtgc tgctgctgct cgacatcggt ggatcgatgg atgatcacgt ccagtcctgt
    10681 gccgagctgt tttccgccgc gcgcaccgag ttcaagcacc tgatgcacta ctacttccat
    10741 aactgcgtat atgaatcgtt atggaaagac aacgcacggc gttcgacgga acgcacgccg
    10801 acgcacgacg tgatgcatac ctatcccgac gactacaagc tgatcttcgt cggcgatgcg
    10861 gcgatgagtc cgtatgaaat cgtccagcgt ggcggcagcg tcgagcactg gaacgaagag
    10921 tcgggggcgg tgtggatgca gcgtctgctc gatacctatc cgcacgcggt gtggctcaat
    10981 ccgctgcccg aggccgcttg gcagcactat cagtcagtcg aaatggtgcg gcatctgatg
    11041 cacggacgga tgtatccact gacgctgcac gggctcgacg atgcgattcg tgagctcagc
    11101 cactaacgat cagggacggg gcaggttgca ggtgccgggg ctcgagtaac aggcgctgtt
    11161 tacgcagtag cctgagcagt cacggctcgt tgcacagggc ttgcggctgc acttgccggc
    11221 gtcgcagcga aaatcggtcg gacagtcact ggcgctgaca caggccttgg gctcgcagcg
    11281 gtggtttgcg gcgcagcgct ggccttcact gcattcgctg tccgccgcgc agccatttgt
    11341 gcagacggtt tcgccgccgc acaggcagag cgccggggtg tttgtgcaaa tcgcggtcgg
    11401 tccagtggcc gcacaggcgg cgtcgctggt gcagatttcc gatggcagtg gccggcgaca
    11461 gattccgcag ccggcaaact gaccggggga caggcaagtg ccttcggcgg gcgtacattc
    11521 ttgttcggtg gcgcagcggc ccatggggat gggcttgggc gggctgtcac acgcggaaat
    11581 cagcagggca gcgagagcaa aggccgtaca caacatggct cgtttcatgg gaagtctcct
    11641 tgggctggcg gattcgatta tcggttggcc cggcggcttt gcttggctgc cacagcgtcg
    11701 ttttttccgt cgacaagtgc tgcggatgaa ttcctttctc gacgaaaaga gggtgacggg
    11761 aaaattttgc ggatttgtgc ttaattcgta gccacattca tgcacgaaag tacgcccgct
    11821 cttcctgctt ctggcaggtc accgacggca accccctcaa gtcgttcgct tggaaattcc
    11881 ccctcttcac cgggcaatgc tccgaccgct cactcgcacg gccacggtcc caaaatcgaa
    11941 aacacgtccg accttgtgaa agtttcgttg gcggcgctcg gcgtcgtcta tggcgacatc
    12001 ggcacgtcgc cgctgtacgc cttcaaagag tgtttcaccg gggaacacgg cgttcccgtc
    12061 gacccggcca acgtctttgg catcctgtcg ctgatttttt ggtcgctgac gctcgtcgtc
    12121 gtcttcaagt acatgacgtt catcatgcgc gccgacaacc acggggaagg cggcattctg
    12181 tcgcttttgg cagtgctggc gccgcgtggc aatgtcgaca agcgagcgat tggccggctt
    12241 tcgctggtgc tgttcggtct gtttggttcg gcgctgctct acggtgacgg catcatcacg
    12301 ccggtcatct cggtgttttc ggcggtcgaa ggtgtgcagg tctacgcgcc gatgataggg
    12361 actggcaagt ttgcggtgtc gacggcgagc ctggtgatgc cgctcactgc gtcgatcctg
    12421 gtcttgctgt ttctgatgca aaaacgtggc accgcaggcg ttggcgcatt gttcggcccg
    12481 gtgatgatgg tgtggttttt tgcgattgca ctgttcggta tcgtcggtgt catccgcaat
    12541 ccgagcgtgc tttgggcgat gaatcccatc cacgccattc ggtttttcgt tcagcacaaa
    12601 atgcacggct ttttggcgct gggtgccgtc gtgctgtgcg tgacgggagg ggaagcgctg
    12661 tacgccgaca tgggccactt tggtcagcgc tcgattcgac tggcttggta cacgctggtc
    12721 ttcccgtcgc tgctgctcaa ctattttggc caaggtgcct atctgctttc acacggaaaa
    12781 cctgaaggca ccttcaaccc gttttatcaa atcgtgccgt cgtgggcgca ggctccgatg
    12841 gtggtggtgg cgacgctggc cacgattgtt gcgtcgcagg cgttgatttc gggcggcttt
    12901 tcgctgacgc ggcaggcggt gcagcttggc tactggccgc gc
//
LOCUS       DAORGN010000144        12711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2055_length_12711_cov_7.264776, whole genome shotgun sequence.
ACCESSION   DAORGN010000144 DAORGN010000000
VERSION     DAORGN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_12711_cov_7.264776"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /locus_tag="PKL17_14930"
     CDS             complement(<1..2072)
                     /locus_tag="PKL17_14930"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNK46077.1"
                     /translation="MSALAWLTAGDAAADPKLRYQIDQRGDMILIGNTVGFDCRSGIP
                     KPVVGTVDTANCGTNIEDSSADVWWRDDAGGSGGAVASPDIKSPDARTTAQLNLPAGA
                     KVTYARLYWAGTYEESSPPDGKVTVERPGQGQRMIVAAPMDIERNYIGGKSYQASADI
                     TGLIQQYGAGQYRIAGVPRMPSANSNSDVIYATWSVVVFYQKDDLPIRNLTLWDGLTG
                     VIGGNKTSVNLSGFRVPMGSKIDAKLAVVAYDGDHDYDGDSLSWNGTRLVDGTTGSEN
                     NFFNSTKTYLGQVVSTTGDLPQLTGDSGSMIGLDLDVVDVSPYLKANDTQALLQLEST
                     KEDIVQLGVIATSIASTKPIIETILTYPQGVSTKPGDVIEFTSNTRNIGDAPGGDLVI
                     EQKLPPGLSYVPESVRMFVGGDPSLNGNKTDKPGDDQVEWDPLTGTLRIRIGKGATAS
                     KGGTIDPTDPPVVVKYQVKIDDRAYGELPLQSTTTATPVGGVSSGPILFPSGNGVTPN
                     APTIVVVPPCVSNDDCSPGAPVCDKSGPQARCTDVCVTDADCKGTPGGAEVCGTMMKC
                     VQCTSAANSACIASGPGNQCISPGYCGCNTDADCGGRTCDVVTHTCPKSLVDLAVMVA
                     HEPQAARKDTPLTYAVSVKNQSSLADSGPVRVTFEVNRGGFVDKLTAQPGWRCSLSDQ
                     KVSCLRYRP"
     gene            complement(2497..3555)
                     /locus_tag="PKL17_14935"
     CDS             complement(2497..3555)
                     /locus_tag="PKL17_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBM20 domain-containing protein"
                     /protein_id="HNK46078.1"
                     /translation="MLGLRFSVFIGLTLVAGEAVGQPKICGNQAALGSWNVDLCPQLK
                     DDGAGADEAAGDGIYSVEISLDPTSVLEYKLLPQGTWALALGQQGTCDGSGNPTGNDT
                     SNLRILRPDVSRPVRFYLDTRTLSDPGAVQPPGNRSFGDTLMTRSPSGECPRWFVVGD
                     FQNLVGVNGTAVPLSLLRPGVLVGKHTASKSLGSGWKWKVVEQADTAARELGGSGWAY
                     APCQAPAASASQPVSPGDTVYFVIDTRTGRLRSVVSNSPLDGFAPDGTALCPPVVDMA
                     SPLSDLAGSAADLAGGSAADLTSAGTADGGSDKPLPGIHCDCQLASTPKTPPPVSLTA
                     VPLLVLLRRLLRSRRSRR"
     gene            complement(3562..4116)
                     /gene="accB"
                     /locus_tag="PKL17_14940"
     CDS             complement(3562..4116)
                     /gene="accB"
                     /locus_tag="PKL17_14940"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNK46079.1"
                     /translation="MTKRTTEESNLDPTAHAGGTTTDGTLTLDAIRSLAQVMKDFELA
                     ELELSQPSGERLRLVGRRAEVIAAAPIATKSAPSVVVAAPEPHHAPAAPPATGKAPAV
                     DKSITVITAPLVGTFYRSASPESPAFVEIGQRVKKGQTLCIIEAMKLMNELECEVEGV
                     VVAILGENGRPVEFGEPLFHVKTG"
     gene            complement(4151..5746)
                     /locus_tag="PKL17_14945"
     CDS             complement(4151..5746)
                     /locus_tag="PKL17_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46080.1"
                     /translation="MHQATSPKRTQRRKTCLGARSRAIVALTATVVWGQVDSALASDF
                     VDTRLNVTLTNENVLAAPGETNPPVPGWRFDRPNQLGVLFFDNYDTRFTGYESLTHIV
                     LYKAIQRQRWEGEAAYVLRLLQFSDVNLSSIDDGSYIKVNYYFDPTRTRRTNISVTAF
                     PLNADRMRLGYSWRLSWGGSPIFFKANPDVPTNLNPPPTPPVPGLRVQLADDRWYAYF
                     GAKTSVLRYEQDQELHSVYGLLGGAGVDLVPGHLRLEANGGFFDRGTIPRVLTQRLPV
                     QTFGVSAQLSAWDGLSPSMSADTALYRNDPNSAARYLTKPIYTPGVSWLLMSEFTAEA
                     TTLENPDQAESSRAQWGLAGDVNFRLQYGNFRYRLDAMYRSLQFLLVNQPSLVPFQDF
                     PSGDGVEIRPEFFAATGADYFVERSGTTIGLTVGVDRPASYKPPTSAVNPVDSTPAIL
                     VVRNQGDVVILPQGQDVLPAIATKLALRQDFLEMFALIAETYYQYDPNGTRLVTDPAT
                     QLKQRVFENPNRLGFNFTLQARF"
     gene            5979..6773
                     /locus_tag="PKL17_14950"
     CDS             5979..6773
                     /locus_tag="PKL17_14950"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46081.1"
                     /translation="MDQSKKTMRHFYCRDALWETFEAMAADFDCSVDYLINEAMRIYA
                     RSKNYQVGSASGSGSQISTAGPGPSVGTPSGQKLDRAQPTTPSPVAPSPTSAFERPPS
                     PDRPPTASFERPPVPATNLGYPPPRPTTGTMPRANALPPPPPPPPSAGPPTPPTGTPG
                     WGAAPGSESPAPPLFLLFQGQRILIDKDQFVIGRGSKTADLAIKDGNISRQHAVVLRR
                     NGVYYIKDLGSTNGIEFKGMRIDNKRIDEGDTFFICDFELRFTYQP"
     gene            6798..6965
                     /locus_tag="PKL17_14955"
     CDS             6798..6965
                     /locus_tag="PKL17_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46082.1"
                     /translation="MRTALSSLGVLLLVQGAVTIILRRLGIIHIFPLAGSLMMLVGYL
                     LWRISRKLGSP"
     gene            6962..7474
                     /locus_tag="PKL17_14960"
     CDS             6962..7474
                     /locus_tag="PKL17_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46083.1"
                     /translation="MKFPFSDGNTAAKCIVALSVLLFGCGSGGAVSPSGPAIGVICIP
                     VGCLSGALYDGTINLGSVDPGSLILSVCRNGVCAKTPLRKDSTGRYVGQIAPPLPVSA
                     WLDSSVTPARLWTYVFGRPELLIDGDVYEISLASASMTLFRIPPKSAVYLRDPQQDPN
                     CAAQCVHVNL"
     gene            complement(7487..7888)
                     /locus_tag="PKL17_14965"
     CDS             complement(7487..7888)
                     /locus_tag="PKL17_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNK46084.1"
                     /translation="MAKKTTFPSNPEDWRKLSFSVGRINQRLEEALSSQCGLLQSEFD
                     LLGVLLHSRAESLRMSELQKQLLLTPSGMTRVVSRLVQKKLLKKLTVSDDKRGFSVAL
                     LPRGLAVYLRAKDVCAEVLNDPELAPLHLRM"
     gene            8295..8945
                     /locus_tag="PKL17_14970"
     CDS             8295..8945
                     /locus_tag="PKL17_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK46085.1"
                     /translation="MFDRQKKQVASPRARFEQEALIHMDALYATALRLTGDARDAEDL
                     VQDSVLQAYRAFDSYEPGTRCKAWMYKILTNTFINKYRRRVLEHAVTSDMERGGDPGL
                     LHESGSRQASDPEDTLHFQLMSDSIARALSALPEEYRLAVLLSDVEEFSYREIADIME
                     CPVGTVMSRLHRGRRLLQSALREHAIRSGIIKDVPSRKSAEPGRIIPLPRRASGKN"
     gene            8954..9733
                     /locus_tag="PKL17_14975"
     CDS             8954..9733
                     /locus_tag="PKL17_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46086.1"
                     /translation="MQCSDLERDLESLVDGELLASDRLEYERHLGECSGCQRLVSSRR
                     ELRRAVREAVSVPCPSDLRAKVSQQLDRHDEVQAAAQGTWWQRTWQQPAVMLVAGALA
                     GLLVVPTLRSGKERNDRPVVVDAMISRHQLPLPLDVTGEPEKVRSWFTGKVPFRVPAP
                     QLEPVASLEGGRLADLGDREAVLLRYQQSGQPISVYIFDPQGLELNTRRTVIGNREVF
                     LPDGRGYQVAVFRDRGLGYAVTGTMNEPDFLKVVSAMVSGR"
     gene            9793..11985
                     /locus_tag="PKL17_14980"
     CDS             9793..11985
                     /locus_tag="PKL17_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNK46087.1"
                     /translation="MPSSSRLGALASLCLSASLVCGHAAAASRGNLVDKQVALIQKAV
                     TAYQSGEYEKTRTLLLPLVDKDQLKGYRSRDYLLFLLGESEALIATETGNVALCGPAT
                     THLRAVEAVPDTPLSGQARARAADCLRLLGKTSDAEAAYRSVLGSSKNTIDLGTARFR
                     QAELLGGQSDEKMGQEARTLLRRIYIEQPMHPLAESALAKLRSLDKDAQLDAQEHLER
                     AKNLLSGRRWIEAAAELQGLPPDLGPTLRDEVDYWLGTTFFRMRRNYGGAAQLLLAVA
                     KRLKGDRQADAMFHGARALSRADRDDEAIVGYRDMLSVHPHSKYSAEASFLIGWLDYN
                     RSRYEQAITSLDETLKRYSSGTYAEEARWYIGWSRYLLGDFAGALPTFSALSKKSGLS
                     GSKGFYWLAVAQLRLGKQQEGLSLLRKLAEREPFSYYGQLSRVRLKEAGQNIPLFIDP
                     VRQSPDGLPEFQLPPDKQVLADDRLRRPEELLLFGLKAEAGQELSRVESPLLQKYGAA
                     KALPVLFDMYHRAGSFRRPHLLAEVHGSAALRKDPHAVPGARSYWEAMYPLAYRGFIE
                     KYGPSGENPRRYLYSIMQKESAYNPYDVSYADAIGLLQMIPPTSRRVAPHIGREYTDD
                     VLYDPEGNIQFGAWYIGRLLRKFKGQIALGAGSFNAGPRAMTRWLERNGSRPLDEFVE
                     LCPYSQTREYMKKLLGIYAHYVYLWDKEEYLPSLKVDKDFLSTDGIDY"
     gene            12018..12695
                     /locus_tag="PKL17_14985"
     CDS             12018..12695
                     /locus_tag="PKL17_14985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNK46088.1"
                     /translation="MIETVMIATPNGPQMAAVAIPKTEGPHPAILICHEWWGLNDDMR
                     RMAERFAGEGYLAIALDFYEGKSTADPGEAMQLVMQLKTDHALAVTKAAVDKIKSDPR
                     CTGRIAITGFCLGGAMAIAAICNVEGIAAAVPFYGLPMPKFQNWQNVKGPILGHYAQN
                     DSHVPAEKVQGVVVAARQAGVTFECHFYSGSHGFMRKSDAAIYHPESADLAWNRTLAF
                     LKQHAAS"
BASE COUNT         2589 a         3903 c         3748 g         2471 t
ORIGIN      
        1 ggacggtagc gcaggcagct tactttctga tccgacagcg agcagcgcca gccgggctgc
       61 gccgtcaatt tgtcgacgaa accaccgcga ttcacttcga aagtgacccg caccggaccc
      121 gaatcagcca gcgagctttg gtttttcacc gacaccgcgt aggtcagcgg cgtgtccttg
      181 cgagcggcct gcggttcgtg cgcaaccatc accgccaaat cgaccaggct cttggggcag
      241 gtgtgtgtga ccacatcgca ggtccgtccg ccgcagtcag cgtcggtgtt gcagccgcag
      301 taacccggtg aaatgcactg gttgcccggt cccgacgcaa tgcaggccga attcgcagcc
      361 gacgtacact gcacgcactt catcatcgtg ccgcagactt cggcgccacc gggagtgcct
      421 ttgcagtcgg catccgtcac acatacgtcg gtgcagcgtg cttgcgggcc gctcttgtca
      481 cagacgggag ccccagggct acagtcgtcg ttgctcacgc acggcggcac caccacaatc
      541 gtcggcgcat tcggcgtcac gccattgccc gacggaaaca ggatcggccc agagctgaca
      601 ccgccaacgg gtgttgccgt cgtcgtgctt tgcagcggca gttccccgta agcgcggtcg
      661 tcgatcttga cctgatactt gaccaccacc ggcgggtcgg tcggatcgat cgtgccgcct
      721 ttgctggccg tcgcgccctt gccaatccga atccgcagcg tcccggtcag cggatcccat
      781 tcgacctgat cgtcgcccgg cttgtcggtc ttgttgccat ttaagctcgg gtcgccaccg
      841 acgaacatcc gcaccgattc gggcacatag ctaagccccg gcggcagctt ctgctcgatc
      901 accagatccc cgcctggcgc gtcgccgatg ttgcgggtgt tcgatgtaaa ttcgatcaca
      961 tcgccgggct tggtgctgac cccctgcgga taggtcagga tcgtctcgat gatgggcttg
     1021 gtgctggcaa tcgacgtcgc gatgacgcca agctgcacaa tgtcttcctt ggtcgactca
     1081 agctgcagca gcgcctgcgt gtcattggcc ttgaggtacg gcgacacgtc gacgacatcc
     1141 aggtcgagac caatcatgct gccgctatcg ccagtgagct gcggcaaatc acccgtcgtc
     1201 gatacaacct ggcccagata ggtcttggtg ctgttaaaga agttgttttc cgaaccagtc
     1261 gtcccgtcga cgagccgcgt gccgttccag ctcagcgaat ccccgtcgta gtcgtgatcg
     1321 ccgtcgtagg ccaccactgc gagcttggcg tcgattttcg atcccatcgg cacccgaaag
     1381 cccgacagat tcaccgacgt cttgttgcca ccgataacgc ccgtaagacc gtcccacagc
     1441 gtcaggttgc gaatcggcag atcgtccttt tgataaaaaa caacaaccga ccatgtcgca
     1501 tagatcacgt cggaattgct gtttgccgac ggcatgcgcg gaacaccggc aatccggtac
     1561 tgaccggcgc cgtactgctg aatcagcccg gtgatgtcgg cggacgcctg gtagcttttg
     1621 ccaccaatgt aattgcgctc gatgtccatc ggcgcagcga cgatcatgcg ctggccctgg
     1681 ccaggacgct cgacggtgac tttgccgtcg ggagggctgc tttcttcata cgtgccggcc
     1741 cagtacaatc gcgcgtaggt gactttggca ccggcgggca ggttgagctg cgcggtcgtt
     1801 ctggcgtcgg ggcttttgat gtcggggctg gccactgcac cgccgctgcc accggcgtca
     1861 tcgcgccacc agacatctgc cgatgaatcc tcgatgttgg tgccacagtt tgcggtgtcg
     1921 accgtaccga cgacaggctt cggaattccg gatcggcagt caaaaccaac ggtgttgccg
     1981 atcaggatca tgtcgccgcg ctggtcgatc tggtaccgca gcttggggtc cgcagcggca
     2041 tcacctgcgg tcaaccaggc aagcgctgac aaggcggtta caagcggtag tctcgccccg
     2101 cggcgacggc caaaccgatc tgggaatgtc ataaagagcc tccttcacag gcgcaagcga
     2161 tggatcgagt ccggttagaa cttcccattt ttgtaaatca aaacacacta aattagcaaa
     2221 tcaaaacgcc ttttcggcag tccatcccaa ggcttagcca gcacccaagc ggccctactt
     2281 tcgacggcaa ttcaccgctt tcgctatctg aaagccaccg tcgcgggtcc gatctccgtt
     2341 taacgaagat tgtttctgta actgctcaag ccaaattgaa atgatgtggt agcagaccca
     2401 acgactgcgc accggatggc acaagccagc acaggtgttc aagcggcgga cgtgacaggg
     2461 aaaagagccg tgcggggggg cagcacaaag cggctgctat cgacgtgatc gacgagaacg
     2521 caaaagccga cgcagcagta ccagaagcgg aaccgccgta agactgaccg ggggtggggt
     2581 ttttggcgtc gacgcaagct gacagtcgca gtggatgccc ggcagcggtt tgtcactgcc
     2641 accgtcggca gtcccggcag aagtcagatc agccgcggaa ccgcccgcaa gatcagccgc
     2701 ggaaccggcc aaatcggaaa gaggactcgc catgtcaacc accgggggac acagcgcggt
     2761 gccatccggc gcaaagccgt cgagcggcga gttcgatacc accgaacgca gccggcccgt
     2821 gcgtgtatca atgacaaagt agaccgtatc accggggctg actggttgac tggccgacgc
     2881 cgctggggct tgacacggag catacgccca gccgctacca cccagctcgc gcgcggcggt
     2941 gtcggcctgt tccacgactt tccacttcca gccactgccg agcgacttgc ttgccgtatg
     3001 tttgccgacg aggactccgg gccgcagcag cgaaagtgga accgcagtgc cgtttacgcc
     3061 aaccaaattt tggaagtctc cgacaacgaa ccagcgcggg cattcccccg acggagagcg
     3121 cgtcatcagc gtatcgccaa aagagcgatt gcccggcggc tggacagcgc ctgggtccga
     3181 cagcgtccgc gtatcgaggt aaaaacgaac cgggcggctt acgtcaggcc gcaaaattcg
     3241 caggttgctc gtgtcgtttc ccgtcggatt gccgcttccg tcacaggtgc cttgttgccc
     3301 aagcgccaaa gcccacgtcc cctgcggcag cagcttgtat tccagcacgc ttgtcggatc
     3361 gaggctgatc tcgaccgagt aaatcccgtc gccagccgct tcatccgcgc cagcgccgtc
     3421 atcctttaac tgcgggcaca gatcgacatt ccaactgccc agcgccgcct gattgccaca
     3481 aatcttgggc tgcccgactg cctcccccgc gacgagagtc agcccgatga agacagaaaa
     3541 acgcagcccc agcatggtgc gtcatccggt cttgacgtgg aaaagcggct cgccaaattc
     3601 gacgggccga ccattttccc cgaggatggc gaccaccaca ccctcgacct cgcactccag
     3661 ctcgttcatc agcttcatcg cttcgatgat gcagagcgtc tggccttttt tgactcgctg
     3721 accgatttcg acaaacgccg gcgattccgg gctggccgaa cgatagaacg tcccgacgag
     3781 aggcgcggta atcaccgtga tgctcttgtc gacggcggga gccttgccgg tggcgggcgg
     3841 tgcagcgggg gcgtgatgcg gctctggcgc agccacaacc accgacgggg cggatttggt
     3901 agcgatcggc gccgcagcga tcacttcggc ccgtcgaccg acgagacgga gccgctcacc
     3961 cgacggctgc gacagctcca gctcggccag ttcaaagtct ttcatcacct gcgcgagcga
     4021 gcgaatcgcg tccagcgtca gcgtgccatc ggtcgtcgtc ccgccagcgt gtgccgtcgg
     4081 gtcgagattg ctttcttcgg tcgtcctctt cgtcatgcgg ttctcactgt gaggctgcga
     4141 ttatgagcga tcagaagcgc gcctggagcg tgaagttaaa gccgagacgg ttcggatttt
     4201 caaacacgcg ctgcttcagc tgggtcgccg ggtccgtcac cagccgagtc ccgttcgggt
     4261 cgtactggta gtacgtctcg gcgatcagcg cgaacatttc cagaaagtct tggcgcagtg
     4321 ccagcttggt cgcgatcgcc ggcagcacat cttgaccctg cggcaaaatc accacgtcgc
     4381 cttggtttct gacgaccaag atggccggcg tcgagtcaac cggattgact gccgaggtcg
     4441 gtggtttgta gcttgcgggt cgatcgacac cgacggtcag accaatcgtt gtgcccgatc
     4501 tttccacaaa ataatccgct cccgtcgcag caaaaaactc ggggcgaatt tccaccccgt
     4561 ccccagacgg aaaatcttgg aacggaacca agctcggctg attgaccagc aaaaactgca
     4621 gtgatcgata catcgcgtcg agccggtagc ggaagttgcc gtactgaaga cggaagttca
     4681 cgtcgcccgc cagaccccac tgcgcccgac tggattcggc ctggtccgga ttttccagcg
     4741 tcgtcgcctc ggcggtaaac tcgctcatca ggagccacga aacgcccggc gtataaatcg
     4801 gcttcgtcag gtaacgcgcc gcgctgtttg ggtcattgcg atacagcgcc gtatccgccg
     4861 acatcgacgg cgacagccca tcccacgccg acagctgcgc cgacacaccg aaggtttgca
     4921 ccggcagccg ctgcgtcagc acgcgcggaa tcgtgccacg atcaaagaaa ccaccgttgg
     4981 cctccagtcg caggtgcccc ggcacaagat cgacaccggc gccgcccaaa agtccgtaca
     5041 ccgagtgcag ttcctgatcc tgctcgtagc gcagcaccga cgtcttggca ccaaagtacg
     5101 cataccagcg atcgtcggcg agctgcaccc gcagacccgg aaccggcggc gtcggcggcg
     5161 gatttaggtt ggtcggaacg tcgggattcg ctttgaagaa aatcggcgaa ccaccccacg
     5221 acagacgcca ggaatacccc agacgcatcc gatcggcatt gagcggaaac gctgtcaccg
     5281 aaatgttggt ccgccgcgtt ctcgtcgggt cgaaatagta gttcaccttg atgtacgagc
     5341 cgtcatcgat cgacgacaag ttcacgtcgg aaaactggag cagacgcagc acgtaggccg
     5401 cttccccctc ccatcgctga cgctgaatcg ctttgtacag cacgatgtgc gtcaggcttt
     5461 cgtacccggt aaagcgcgtg tcgtagttgt cgaaaaacag cacgccaagc tgattcggac
     5521 gatcaaaccg ccagcccggc accggcgggt tcgtttcccc tggagccgcc agcacgtttt
     5581 cattcgtcag cgtcacgttg aggcgcgtat cgacaaagtc gctcgccagc gccgaatcaa
     5641 cctgccccca aacgaccgtc gcggtcagcg ccacgatggc ccgtgatcgc gcccccaaac
     5701 aggtcttccg tcgctgggtc cgtttcggcg aagtagcttg gtgcattcct atgggctcct
     5761 aattgacgca gtttgttatc acggaggcgg ccaaagtgcc aaacttcgcg cccacatctt
     5821 gatgtcgcgc gcttgtgagg ccatgctctc gggggcgacc ttgacctttg cgatctaggg
     5881 ggttaggcta cccgccaggc tatgtggcct cgcaagtttg tctgtgagta gccttgccac
     5941 cgggcatctt cgcaaggcgt gataacgagg tacttggaat ggaccaaagc aagaagacga
     6001 tgcgccactt ctactgccga gacgcgctct gggagacgtt cgaggcaatg gccgccgact
     6061 tcgactgctc ggtcgattac ctcatcaacg aagcgatgcg gatctacgct cgctcgaaga
     6121 actaccaagt tgggtctgca tcgggatcag gcagccagat ttcgacggct gggcctggtc
     6181 cttccgtggg aacgccgtcg gggcaaaaac tcgatcgggc gcagccgacg acgccatccc
     6241 ccgtggcacc atcaccgacc tcggcgtttg agagaccccc ctccccggat cggcctccga
     6301 ccgccagctt tgaacggccc ccggttccag cgacaaacct tggttatccg ccgccgcgtc
     6361 cgaccaccgg aacgatgccg cgcgccaacg ctttgccgcc gcccccaccg cctccgccga
     6421 gcgccggacc accgacgcca ccgacgggaa caccaggctg gggagccgcg cccggcagcg
     6481 aaagtccggc tcccccactt ttccttttgt tccaaggcca gcgcatcctg atcgacaaag
     6541 accagtttgt gatcggacgc ggcagcaaga cggcggactt ggcgataaaa gacggcaaca
     6601 tctcgcgcca gcatgccgtc gtgctccgtc gcaacggtgt ctactacatc aaggatctgg
     6661 gctcgaccaa cggaatcgag tttaagggaa tgcggatcga caacaagcgc atcgacgaag
     6721 gcgacacgtt cttcatctgc gacttcgagc tgcgtttcac ctaccagccc taagcggatt
     6781 ttgcggctcc ttacgcgatg cgtactgcgc tttcttctct cggtgtgctg ctgctcgttc
     6841 agggtgctgt gaccatcatt ttgcgccgct tgggaatcat tcacatcttc ccgctcgcgg
     6901 gttcgctgat gatgctggtc ggctatctgc tgtggcgcat ttcgcgcaag ctgggaagtc
     6961 cgtgaaattc ccattttccg acgggaacac cgctgcgaag tgtattgtcg cgctgtctgt
     7021 cctgctgttc ggctgtggat cggggggcgc ggtatcaccg tcgggccctg cgattggtgt
     7081 gatctgcatt ccggtcggct gtctaagcgg cgcgctttac gacggcacca tcaacctggg
     7141 cagcgtcgat cccggcagct tgatcttgtc ggtctgccga aacggagtct gcgccaaaac
     7201 gccgctccga aaagacagca caggtcgcta cgtcggacaa attgcgccgc cgctcccggt
     7261 cagcgcttgg ctcgattcca gcgtgacccc tgctcgcttg tggacgtatg tctttggtcg
     7321 gcccgagctg ctcatcgacg gagatgtcta cgaaatcagc cttgcatctg cgagcatgac
     7381 cctcttccgc attccgccca aatccgcggt ctacctgcgc gatccgcagc aagatccgaa
     7441 ctgcgccgcg cagtgcgtcc acgtcaatct gtagcgcctc acgctgtcac atccgcagat
     7501 gaagtggcgc aagctccgga tcgtttagca cctcggcaca gacatctttt gcccgcagat
     7561 agaccgccaa accacgcggc aaaagcgcca ccgaaaaacc gcgcttgtcg tcggaaactg
     7621 tcagcttttt cagcagcttt ttttgcacca accgactgac tacacgggtc attccgctgg
     7681 gtgtcagcag cagttgtttt tggagctcgc tcatccgcag cgattccgcc cggctgtgaa
     7741 gcagaacccc cagcagatca aattcactct gtaacagacc acactggcta gacagcgctt
     7801 cttccagtct ctgattgatc ctaccgacgg aaaaagacag ctttcgccag tcttctggat
     7861 tgcttgggaa agtggtcttt ttggccattg cttgcgcaag caaccacatc tttgtttgcg
     7921 caagcaacca ctttgttgac tggtcaacaa tctccacaac ctgtggataa gcttgtggat
     7981 aacgtgattt tggcgattgg gagtcgtttt gacggcccaa aaaccgtcaa aaaacccaag
     8041 caggccgatt attggtccga ccaaattttc cttcttcgga tcgaactggc ccaaccaatg
     8101 tgagcggtgc gaaaacgtcc gccgaaaaag tgcttgctcg gggtgtgatc gcgacgatgg
     8161 cgcgatccag aaacgcccga tcttccgtcg gaaaacgcga aaaaaaggca gcattggcga
     8221 cggagcggca aagatcgcga aatttttgcc gagacccctt ggaataggtt tcgcgcgccc
     8281 tacgttaccc aggcatgttc gatcgccaga agaagcaggt ggcctcgccg cgagcgaggt
     8341 ttgaacaaga ggcgctcatc cacatggacg ccctgtatgc gacggcgctg cgactcacgg
     8401 gggatgcccg cgacgccgaa gacctggttc aagattcggt gctccaggcc taccgcgcgt
     8461 tcgacagcta cgaaccggga acgcgctgca aggcctggat gtacaaaatc ctcaccaaca
     8521 cgtttatcaa caagtatcgt cgccgcgtgt tggaacacgc agtgacgtcc gatatggaac
     8581 gtggtggtga tccggggctg ctccacgaaa gcggctcgcg gcaggccagc gatcccgaag
     8641 acacgctgca ctttcagctg atgtccgatt cgatcgcgcg ggcgctgtcg gcactaccgg
     8701 aagaatatcg cctggcggtg ctgctgtccg acgtagaaga gttttcctac cgcgagatcg
     8761 ccgacatcat ggaatgtccc gtgggcaccg tgatgagtcg gctgcatcgt ggccggagac
     8821 ttttgcaatc cgcgctgcgc gaacatgcga tccgctcggg tattatcaag gacgtgccga
     8881 gtcggaaatc cgccgagccg ggacgaatca ttccacttcc gcgacgagcc agcggcaaaa
     8941 actaattaca gccatgcagt gtagcgatct cgaacgggac ttggagagct tggtagacgg
     9001 tgagctgctc gcttctgatc ggctcgaata cgagcgacat ctgggcgagt gttcggggtg
     9061 tcagcgactc gtgtcctcgc gacgggagct tcggcgagca gtgcgcgagg cggtgtcggt
     9121 gccgtgtccg agtgatctgc gcgccaaagt gagccagcag ctcgatcgac acgacgaggt
     9181 ccaggccgct gcacaaggca catggtggca acgaacttgg cagcagcctg cggtgatgct
     9241 cgtcgctggg gcgctggccg ggctgttggt agtcccgacg ctgcgatcgg gaaaagaacg
     9301 caatgatcgg cccgtggttg tcgacgcgat gatctcgcgc catcagcttc cgctcccgct
     9361 ggatgtgacg ggcgaaccgg aaaaagtccg atcgtggttt accggcaaag tgcccttccg
     9421 ggtgcccgcg ccgcagcttg agcccgtcgc gtcgcttgag ggcggacgac tggccgactt
     9481 gggcgatcgc gaagcggtgc tgcttcgcta tcagcagagc ggtcagccga tctcggtcta
     9541 cattttcgat ccgcaggggc tggagctaaa cacccgtcga accgtcatcg gcaatcgcga
     9601 agtctttctg cccgacggtc gcggctacca agtcgccgtc tttcgcgatc gtggactcgg
     9661 ttacgccgtc accgggacga tgaacgagcc agactttctc aaagtcgtca gcgcgatggt
     9721 cagcggtcgc taaacgcgaa agtttcgtct gacatcacca ggctcggcta tcatctctgg
     9781 ctggtgtttt ccttgccctc cagctcccgt cttggcgcgc ttgcgtcgct gtgcttatcg
     9841 gcttctcttg tttgcggaca tgccgccgca gcaagtcgtg gaaacctagt cgacaaacag
     9901 gtcgcgctga tccaaaaagc cgtcaccgcc tatcaaagtg gcgaatacga aaaaacccgc
     9961 acgctgctgc tgccgctcgt cgacaaagac cagctaaaag gctaccgaag ccgcgactat
    10021 ttgctgtttt tgcttggtga aagtgaagcg ctgatcgcga cggaaacagg caatgtggcg
    10081 ctgtgtggtc cggcgacgac ccacctgcgc gccgtcgaag cagtcccaga tacgcctctt
    10141 tctgggcaag caagagcgcg ggctgccgac tgtctgagac ttctgggcaa gacaagcgat
    10201 gccgaagcgg cctatcgcag tgtgctcggc agcagcaaaa acaccatcga cttgggcacc
    10261 gctcgcttcc gtcaggcaga gctgctcggt ggccagtcgg acgaaaaaat gggacaggaa
    10321 gcgcggacgc ttctgcgcag gatctacatc gaacagccga tgcatccgct ggcggaatcg
    10381 gcgctcgcga agctgcgctc actcgacaaa gatgcccagc tcgacgcgca agagcacctg
    10441 gaacgggcaa agaatctgct gtcgggccga cgctggatcg aagcggcggc ggaactccag
    10501 ggcctgccac cggatcttgg gccgacgctg cgcgatgaag tcgattactg gctggggacg
    10561 acgttttttc gcatgcgtcg caactatggc ggggcggcgc agcttctttt ggcggtggcc
    10621 aagcggctca aaggcgatcg ccaagccgat gcgatgttcc acggggcgcg ggcactgtca
    10681 cgcgccgatc gcgacgacga agccatcgtc ggctaccgcg acatgctgag cgtccatccg
    10741 cactcgaaat attccgccga agcgagcttt ttgatcggct ggctcgacta caaccgcagc
    10801 cgctatgagc aagcgatcac cagcctcgat gaaacgctca agcgctacag cagcggaacg
    10861 tatgccgaag aggcacgctg gtacattggc tggtcgcgct atctgcttgg ggactttgcc
    10921 ggggcgctgc cgacgttttc tgcgctgtcg aaaaagagcg gactcagcgg cagcaaaggc
    10981 ttttactggc tggccgtcgc gcagcttcgg cttggcaagc agcaggaagg tctttcgctt
    11041 ttgcgcaagc tcgccgagcg ggagccgttt tcctattacg ggcagctaag ccgggtgcgg
    11101 ctcaaggaag cgggacagaa cattccgctg ttcatcgatc cggtgcggca aagtcccgac
    11161 ggactgccag agtttcagct cccgccggac aaacaggtgc tcgctgatga caggctgcgg
    11221 cgtccggaag agcttctgct gtttggtctg aaagcggagg ccggccaaga gctatcgcgg
    11281 gtggagtcgc cgcttttgca aaagtacggc gcagcgaaag cgctgccggt gctgttcgac
    11341 atgtaccatc gcgccggcag tttccgtcgc ccacaccttc ttgccgaggt ccatggcagt
    11401 gcggcgctgc gcaaagatcc ccacgcggtt ccaggggcgc gttcctattg ggaagcgatg
    11461 tatccgctcg cgtatcgcgg ctttatcgaa aagtacggac cgagcggaga aaatccgcgc
    11521 cgctacctgt attcgatcat gcaaaaggag tccgcgtaca acccctacga tgtgtcgtat
    11581 gcggatgcga ttgggctcct tcagatgatt ccaccgacga gccgtcgcgt cgctccacac
    11641 atcggaaggg aatatacgga cgacgttctg tacgacccgg aaggcaacat tcagtttggt
    11701 gcgtggtaca ttgggcgatt gcttaggaag ttcaaaggac agatcgcgct gggtgcggga
    11761 tctttcaatg cgggaccgcg ggcgatgacg cgctggctcg aacgaaatgg cagccgtcca
    11821 ctcgatgaat tcgtcgagct gtgcccgtat tcccagaccc gcgagtacat gaaaaagctg
    11881 ctcgggatct acgcccacta cgtctatctg tgggacaaag aggaatactt gccgagtctg
    11941 aaagtcgaca aggatttcct ttccaccgac ggaattgact actaaccaag cgacagagat
    12001 gggggaacgg aaaagcgatg attgaaacgg tgatgattgc gactcccaac ggtccacaga
    12061 tggcggcggt cgcgattccc aagaccgaag gaccgcatcc ggcgattctg atctgccacg
    12121 agtggtgggg actgaacgac gacatgcgac ggatggcgga gcgttttgct ggcgaaggat
    12181 atttggccat cgcgctcgat ttttacgaag gaaagtcgac tgccgatccg ggcgaagcga
    12241 tgcagcttgt gatgcagcta aagaccgatc acgcgcttgc tgtgaccaaa gctgccgtcg
    12301 acaaaatcaa gtccgatccg cgctgtaccg gacgcatcgc gatcaccgga ttctgtctgg
    12361 gtggtgcaat ggcgattgcg gcgatctgca atgtggaagg aatcgcagcg gcggttccgt
    12421 tttacggact gcccatgcca aagttccaga actggcaaaa cgtcaaaggt ccgatcctgg
    12481 gtcattacgc gcagaacgat tcccatgtgc cggcggaaaa agtacaggga gtcgtcgttg
    12541 ccgcgcgtca agccggagtg acatttgaat gtcacttcta cagcggcagc cacggcttta
    12601 tgcgcaaaag cgatgcagcc atctatcacc ccgaatcagc agaccttgcg tggaaccgca
    12661 ccctcgcgtt cctaaaacaa cacgcagcgt cgtagtcctt tcagacggaa a
//
LOCUS       DAORGN010000145        12653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2065_length_12653_cov_5.035482, whole genome shotgun sequence.
ACCESSION   DAORGN010000145 DAORGN010000000
VERSION     DAORGN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_12653_cov_5.035482"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2707
                     /locus_tag="PKL17_14990"
     CDS             <1..2707
                     /locus_tag="PKL17_14990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46089.1"
                     /translation="YAAYDPDLNRKVAIKLLRSQPGAMVSQGRARLLREAQSMARLSH
                     PNVVAVYDVGVAFDQVFVAMEFVEGMTLGEWLSKPRSTDEILQVFAEAGQGLAAAHDA
                     GIVHRDFKPDNVMIGRDGRARVMDFGVARDVSQPISDPGPMLVPTRSAVALDLTLPEQ
                     SSRGLAALAVSSPLTQEGALMGTPRYMSPEQFRGQASDARSDQFSYCIALYEAVCGKP
                     PFAGDNLGELAMQVFLGQVRPFSPEKKVPNWLKKVLLRGLKVQANQRYPSLQALLEDL
                     SPERRRRRRRMLLLLSSCAALVVAGLLAGGAYRGNQALKAQRCQAVDVQLDPVFGAAA
                     KAAAREGLAKTKRPYADDVWQRVENNLSHYLFSWRAMRRDTCQATFVAGTQSAELHAR
                     RLRCLDGRLQEVAAFMNLLRTADEQVLDSAVDQSSDLTLLEPCADPHFLQQLVGEKPL
                     ERDRLDVLSRQLSEARLLHNSGRVQAAKDRAIEVLSAAQQIDNLPLQARAERELSMLE
                     TSGGDPFAAHQRALSAAALGVRGGDFLTAAWAFTDLMYLSQVTQRGISVDKWQQFAES
                     TLARVEGSAQVKARIQATLDLYLCQHLSAQRQKLKADEQCLLALRENERVFGPEASQM
                     GTVLNSLAMHNRRQERYPEAESLYRRALTVSEAHQGSEHPRLVTIHENLGVLLELMGK
                     PEAAEAEYRRALQIAERAFGAEHPKLYGTLKQLVLLLLRQNQAATAIPIAERAAALHK
                     GTPRAVGGRLLLAQALQKGGRFADALAIHEEALAQCSGKNCPDLSTRLLGVADDLRGL
                     GKDKQALPLLEQALRILPPLPTPPQLLPPSELETRARLLQSLCETLWAVDGEKSRARV
                     MELGTQSLKLLGDLGKLHRSDADALHTWLQKSKLLPVERSALAEP"
     gene            complement(2735..3766)
                     /locus_tag="PKL17_14995"
     CDS             complement(2735..3766)
                     /locus_tag="PKL17_14995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK46090.1"
                     /translation="MHPKVAMFANRLRKNLKRLLPWAAQRCVEAYRIYDRDIPEVRLV
                     VDRYADTLVVYPYAYSTELTDEEQAEQDSFHDELIETLSTLTGVPRERIFKKERKRQK
                     GSAQYERLGESKHEMVVHENGHRFLVNLSDYLDTGLFLDHRDTRALLQKMSAGRRVLN
                     LFGYTGSFSVYAAKGGALSTVTVDLSPTYLAWAQRNLQLNGLNLSQHRQVRADVLAWL
                     RDPLSVPELRGPFDLIVLDPPTFSNSKRMHGTFDLKRDHEWLLVSTARFLAPGGQLLF
                     STNRRGFRLKPEEFPTLRIKDISSLTLPRDFHDPLVRVCYLIEPDPSAVQKKPPRPSS
                     SGWFQSERW"
     gene            complement(3810..4937)
                     /locus_tag="PKL17_15000"
     CDS             complement(3810..4937)
                     /locus_tag="PKL17_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNK46091.1"
                     /translation="MKRRTVLALCGAALSLPHKLIASPPKSTPPAGPTQTTLETSVVR
                     LQLRHTWTTVMSTSTFRDVVQVRIHRDGLTGMGEGAPIPRYHETATSALQALETVRGL
                     VEHADPMLHVPLLAEVGKRLPGEWAAKAALAGALADWIGQKLGVPLHRFLGLDGSATP
                     ITTMSIGIDTPDMTRQKVQEAADFPVLKIKVGLDSDETTIAAVRQVTSKPLRVDANEG
                     WKSKEVALRKLDWLAKQGVELVEQPLPAAQIADAKWLKARSPLPIFADESCLRPSDIP
                     RVVETCHGINIKLDKCGGVFEAQKMIAVARAHGLKVMVGCMISSSVSIAAAAQITPLV
                     DFADLDGFLLIANDPMESTTVRRGKLILPNRPGLGLIPRAS"
     gene            complement(4934..5788)
                     /locus_tag="PKL17_15005"
     CDS             complement(4934..5788)
                     /locus_tag="PKL17_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNK46092.1"
                     /translation="MHRQTRSALASDFKRPGRILALLALTLGGISTTSAFEHGPTGQW
                     ELVRNDGGIEVYRRTVGNSPLHEFQGTGMIEAPVTAVLGVLDDVDHRLEWMAEAVAQT
                     LISRSGDRDQTFYSRTGAPWPVSDRDVVIRAVTSVDMKAKMVKIEMNSVEHPTWPPQK
                     GVVRMPHLCGHWFLWPSNGGKWTRVEYQVHADPGGSLPTSIINTVSKKIPYHTIVGVQ
                     KQVVKRKYPEYEQKLAQMPEYKEVLPLLPGLTVKPAAAPSPSPTPAAQPPASPLPAGT
                     PNAAGGGK"
     gene            complement(5818..7146)
                     /locus_tag="PKL17_15010"
     CDS             complement(5818..7146)
                     /locus_tag="PKL17_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNK46093.1"
                     /translation="MLAACASRRATLLERLPKNACAVLFSGREERRNADNHHRFRQSS
                     DFLYLTGMTEPESVAVFTPGKDRRFTLLCRPRDKFAELWYGHRAGLEGATELYGADQS
                     FAISELERRRLELLDGCDEVHYLIGDRPEHDELIGKTIARLRRNERNGSRAPSRIVDL
                     KNTLHEMRLVKDEAAILSMRRSCAVTVEAHLLAMRACSAGKREYEIEALVDYGFRRRN
                     GLPGYQSIIAAGKNATVLHYVDNQDQLEPGQLLLVDAGCELDGFTGDVTRTYPIAAPG
                     HRAQFSREQRQLYDIVLDAQLAAIAAVRPGVTVEELHTLICHRLASGLCALGLLSGDP
                     ASAVESGALKRFYPHRSSHFLGLDVHDVGRYFPDGEARQLLPGTVFTIEPGLYVQPDD
                     PLGPEWRGIGIRIEDDILITTTGHEVLTADLPKRPDEVLKLASTGVNLLV"
     gene            complement(7201..9531)
                     /locus_tag="PKL17_15015"
     CDS             complement(7201..9531)
                     /locus_tag="PKL17_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK46094.1"
                     /translation="MSHLFRRWTLLILTASALACSTPNSNTDPGDNTDLGDKPPEGGP
                     CVTLAEPAAGATCPVTVRYVPAKSVRSVAIAGEWNGWSTSAQTLSGPDGSGAYTISIS
                     LPPGVYGYKLVEDGASWVLDAQNAYRKYVDGTENSGLRVANCFVPKLSVEPGSLQITR
                     TSAGHGEVIAKLKVQSPSHAMTPGVCQIQSQIRKPDTIVPGSLPSLSSLELKIAPDRQ
                     SAELHLRDLADGKYLVSLTPSAGGKNGESLLLPLWIEPEPFRFSDTPLYMAMTDRFVD
                     GDGINPGQIGGVLPEANFRGGDLMGVTQQIEAGYFDKLGVKALWLSPFAMQPATAHLD
                     QSGKYRVAAYHGYWPTLARKVDDRIGGDDALQKLVQAAHRHGIRVLMDAVLNHVHEQH
                     EYFQDPQKRSWFRTGCICGSPGCDWTEKRLSCLFASYMPDIDWTVTDASEQFISDTLW
                     WLEHFNLDGLRVDAVKHVEDAAIFNLTARVRERFEQAGTRYYLLGETAMGWREGDVAT
                     NQSEYDTIKRYMGPNGLDGQFDFVWYHSSAYRVFAYDEKRFLHLDYWTHASLDQFKGS
                     TMVNYLGSHDTSRFATMCTYRDPTPGSAFDRNIANNKWDKLPQAPLDDEPYDRQWLGA
                     LALFTMPGMPLLYYGDEYGEFGGGDPDNRHRLRIDAQLSPKEKKQLERMTRLLSARQS
                     LRGLRRGKLLTVLLTEDVYAYGRPDDDAKQSALVVLSRLSYPTSTQVPIPSELGFAPG
                     STLKDQLSGQTYTAPAPGGRLSVSVPARGGVILSPQ"
     gene            complement(9563..12292)
                     /locus_tag="PKL17_15020"
     CDS             complement(9563..12292)
                     /locus_tag="PKL17_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNK46095.1"
                     /translation="MGKRRVPHFLLWLLIAHSACTQNTPAPSTLTGPEPEREVRPRPR
                     QEAPRLPPIAPPPGDKLWARLTDLAGLAELIPTGPQPGLSSILASLETPYKLVSPYSG
                     LRGDTALRVLEVAIGIPNVETYPEPAKETTAPPRFDPVWNRYRGVYESKLSLLLPTAS
                     CYRFRLALPTLGTLVFHSALLPKKTDNGKAPVELSLSIDDRPVWSEKQTTASRWDRHE
                     VSITAPVGESANTQHDVKLCGRSDGSPVGIPVIGNPELWAKGAGAAAPNLLLIIVDTL
                     RADAVSAMPEVQKFAQKGARFEQAITSATWTRPALLSLLGGDLPSAVGQNAEDMIPKE
                     RDRQRFYALDRKLLPRVLRESGVKVTAIGNNFFLLGYPQIGLSLGFDEVADVRHPVAD
                     SPAITRAALRYLQENRERSFFLQLHYDAPHWPYSPPPQFLPKASTEQAAYMAGRGNSP
                     QPVASEPSARSYLAEAAYADAQIAQVLAELDRLALSSRTMVVIVGDHGEVFDINHNHF
                     VLALKQPTLYHHGWSAYDEILRVPMILAMPGRLPQGVVVKNQVRLFDVAPTVLEVLGL
                     WEKRASLPSGNLQQGQSLLPLMQGEAESTERAAFVEGQNVRALRSLGYLYLRRSDPRL
                     KPLEAASALFQVPEELYDLRDDPQQHRNLLQGPKSPLVEQTLRTMREQFSRHAPRLPD
                     AKMPVTHLALAPGPTSRQLHGLVMSSDLQLAVAGVRDGEVTPLRPGQLEVLLQPGGQL
                     DVHMDPDAKLDLQLTLDGLPLAKEQLLLGPYSLPLLSQGTTPQPVAAGHEGTPDAKPP
                     DSSEPLTLTLQGTILGRLFAAYPPVLGERGDVLLWRDQPSPGAIATSLSRGGGSTQSE
                     VATVMRDWGYAQPEKTQQNTQPGPAPAPTPNQPGAIPSRPVPKETPPSQAAPK"
     gene            complement(12368..>12653)
                     /locus_tag="PKL17_15025"
     CDS             complement(12368..>12653)
                     /locus_tag="PKL17_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46096.1"
                     /translation="LSFVVQDLDYDKVLKLVYSTDGWATSQEIPMGAAGSKNTLYWKT
                     DIDQDFERWQVDVDLPGSFSTFSYKLVYRHGTGGGASPAEFSYSSGLAKM"
BASE COUNT         2318 a         3827 c         3969 g         2539 t
ORIGIN      
        1 ctacgcggcc tacgatcccg acctaaaccg caaggttgcg atcaagctgt tgcgcagtca
       61 gccaggcgcg atggtgtcgc agggtcgggc gcggctgctt cgtgaagcgc agtcgatggc
      121 gcgtttgtcg catcccaatg tcgtcgcggt ctatgacgtc ggtgttgcgt tcgaccaagt
      181 gtttgtcgcg atggagtttg tcgaaggcat gacgctcggc gagtggctga gcaagccgcg
      241 gtcgaccgat gaaatcctgc aagtcttcgc cgaagccggg caaggccttg ccgccgccca
      301 cgacgccggg atcgtccacc gcgatttcaa accggacaac gtgatgattg ggcgggatgg
      361 ccgggcgcgg gtgatggact ttggcgtcgc gcgggatgtc agccagccga tttcagatcc
      421 ggggccgatg cttgtgccga cgcgctcggc ggtggcgctc gatttgacgt tgcccgagca
      481 gtccagtcga gggctcgcgg cgctggcggt tagttcgccg ctgacgcaag agggcgcgct
      541 gatgggcacg ccgcggtaca tgtcacccga gcagtttcgc ggccaagcca gcgacgcacg
      601 cagcgatcag tttagttact gcatcgcgct gtacgaagcg gtgtgtggca agccgccgtt
      661 tgcgggcgac aacctcggcg agctggcgat gcaggtgttt ttgggtcagg ttcggccctt
      721 ctcgccggaa aagaaggttc caaactggct caaaaaagtg cttctgcgcg ggctcaaagt
      781 gcaggcaaac cagcgatatc cgtcgctgca agcgctgctc gaagacttgt cgcccgagcg
      841 acggcggcgt cgtcgtcgga tgttgctgct gctgtcgtcc tgtgcggcgc tggtcgtcgc
      901 ggggctcttg gccgggggcg cgtatcgcgg caaccaggcg ctcaaagcgc agcgctgtca
      961 ggcggtcgat gtgcagctgg atccggtgtt tggtgccgca gccaaagccg ccgcgcgtga
     1021 ggggcttgcc aagaccaagc gtccctatgc cgacgatgtg tggcagcggg tcgaaaacaa
     1081 cctgtcgcac tatcttttta gctggcgggc gatgcggcgc gatacctgtc aggcgacgtt
     1141 tgtggccggc acgcagagcg ccgagctgca tgcgcggcgg ctgcgctgtc tggacgggcg
     1201 actgcaagaa gtggccgcgt ttatgaatct tctgcggact gccgatgaac aggtactcga
     1261 tagtgccgtc gaccagagca gtgatctgac gctgcttgaa ccgtgtgccg atccgcattt
     1321 tttgcagcag ctcgtcgggg aaaaaccact cgaacgcgat cggctcgatg tgttgagtcg
     1381 tcagctctcg gaagcgcgac ttttgcacaa ctctgggcgg gtgcaggcgg cgaaagaccg
     1441 tgcaattgaa gtgctgtcgg cggcccagca aatcgacaac ctgccgctgc aagcgcgcgc
     1501 cgagcgggag ctgtcgatgc tcgaaacgtc gggcggtgat ccgtttgcgg cgcatcagcg
     1561 ggcgctgtcg gcggcggcac tgggcgtgcg gggcggcgat tttctgacgg cggcgtgggc
     1621 gtttaccgat ttgatgtatt tgtcgcaggt gacgcagcgc ggcatttccg tcgacaaatg
     1681 gcagcagttt gccgaatcga cgctggcccg tgtcgaaggc agtgcccagg tgaaagcgcg
     1741 gattcaggcg acgctcgatt tgtatctgtg ccagcacctg tcggcgcagc ggcagaagct
     1801 gaaagccgac gagcagtgtc ttttggcgct gcgcgaaaac gagcgggtgt ttggaccgga
     1861 agccagccaa atggggacgg tgctaaacag cctggcgatg cacaaccggc ggcaagagcg
     1921 ctatccggaa gcggagtcgc tgtatcggcg ggcgctgacg gtcagtgaag cgcaccaggg
     1981 cagcgagcat ccgcggcttg tgacgatcca cgaaaacctc ggtgtgctgc tcgaacttat
     2041 gggcaaaccc gaggcggcgg aagcggaata tcggcgagcg cttcagattg ccgagcgggc
     2101 gtttggcgct gaacatccca agctgtacgg gacgctgaaa cagctggtgc tgcttctgct
     2161 gcgtcaaaac caagcagcga cggcgatccc gattgccgag cgagcggcgg cgcttcacaa
     2221 aggcacgccg cgcgcggtgg gtgggcggct gctgcttgcg caagcgctgc aaaaaggcgg
     2281 acggtttgcc gacgctttgg cgattcacga agaggcgctg gcgcagtgca gtggcaaaaa
     2341 ctgtcctgat ttgtcgacgc gcctgttggg tgtcgccgac gacttgcgcg gtcttggcaa
     2401 agacaaacag gcgctgccgc ttttggaaca agcgctgcgg attttgccgc cacttccgac
     2461 gccgccgcag cttttgccac ccagtgaact ggaaacgcgg gcgcggcttt tgcaatcgct
     2521 gtgcgaaacg ctgtgggccg tcgacggtga aaagagcagg gcgcgggtga tggaattggg
     2581 aacgcagtcg ctcaagctgc ttggggactt gggcaagctg catcgcagcg acgcggacgc
     2641 tctgcacacc tggctgcaaa aatccaagct gttgccggtc gaacgttcgg cgctggccga
     2701 gccctgatgt cccgcacgaa gctttacaga atcgctacca gcgctcgctt tggaaccaac
     2761 ctgacgacga cggacgcggc ggcttttttt gcactgcact tgggtccggt tcaatcaggt
     2821 aacagacgcg aaccagcgga tcgtgaaagt cacgcggcaa ggtcaagctg ctgatgtctt
     2881 tgatgcgcag cgtcgggaac tcttcgggtt ttagccgaaa gccgcgccgg ttggtcgaaa
     2941 acaaaagctg cccaccgggg gccaaaaaac gcgccgtgct cacaagcagc cactcgtgat
     3001 cccgctttag gtcgaacgtg ccgtgcatcc gcttgctgtt cgaaaacgtc ggcggatcga
     3061 ggacgatcaa gtcaaacggc ccgcgcagct ccggcacgct cagcggatca cgcagccagg
     3121 ccagcacgtc ggctcggacc tgccgatgct gcgacaggtt cagtccattg agctgcaagt
     3181 tgcgctgtgc ccacgccaga tacgtcggcg aaagatccac cgtcaccgtc gaaagtgcgc
     3241 cccctttggc tgcataaacg gaaaagctgc cggtgtagcc aaacaagttc agtacccgac
     3301 gacccgccga cattttttgc agcagcgcgc gtgtgtcgcg gtgatccaaa aacagcccgg
     3361 tgtcgaggta atcgctcagg ttcaccaaaa atcggtggcc attttcgtgc accaccatct
     3421 cgtgtttgct ctcgccgagc cgttcgtact gggcgctgcc tttttgccgc ttgcgctctt
     3481 ttttgaagat gcgctcccgc ggcactccgg tcagcgtcga cagcgtttcg atgagctcgt
     3541 cgtgaaaact gtcctgttcg gcctgttctt cgtcggtaag ctctgtcgaa tacgcatacg
     3601 gatagacgac cagcgtatcg gcatagcgat cgacgaccag ccggacttcg ggaatgtcgc
     3661 ggtcatagat ccggtacgcc tccacgcaac gctgcgccgc ccacggcaga agccgcttga
     3721 ggtttttgcg caggcggttg gcaaacatcg cgactttggg gtgcatggcg ccgtttttac
     3781 cactgtccgg cgggcaccac ttcaaagttt cacgacgcgc gcggaatcag cccgagcccc
     3841 ggacgattgg gtaggatcag cttgccgcgg cgcaccgtcg tcgattccat cggatcgttt
     3901 gcaatcagca aaaagccgtc gagatccgca aagtcgacaa gcggtgtgat ctgcgccgcc
     3961 gccgcaatcg acaccgagct tgagatcatg cagccaacca tcactttgag cccatgggcc
     4021 cgtgcgacgg cgatcatctt ttgcgcttcg aaaacgccac cgcacttgtc gagcttgatg
     4081 ttgatgccgt ggcaggtctc gacgacgcgc ggaatgtcgc ttgggcgcag gcacgactcg
     4141 tcggcaaaaa tcggcagcgg tgatcgcgct ttgagccact tggcatcggc gatttgtgcc
     4201 gccggcagcg gttgctcgac cagttcgacc ccttgtttgg ccagccagtc gagcttgcgc
     4261 agcgccactt ctttgctttt ccaaccttcg ttggcgtcga cgcgcagtgg tttgctggtc
     4321 acttgacgca ctgcggcaat ggtcgtttcg tcgctgtcca gtccgacttt gatcttcagg
     4381 accggaaaat cggcggcttc ctgcactttt tgccgtgtca tgtccggcgt gtcgatgcca
     4441 atcgacatcg tggtaatcgg cgtcgcactg ccatcgagac ccaagaagcg atgcagcggc
     4501 acccccagct tttgcccgat ccaatcggcc agcgcgccag ccagcgctgc tttggccgcc
     4561 cactcaccgg gcaggcgctt gcccacttca gcgagcagcg gcacatgcag catcgggtcg
     4621 gcgtgttcta caagcccacg caccgtctcc agcgcttgga gcgccgacgt cgctgtttcg
     4681 tgatagcgcg gaatcggcgc accttcgccc atgccggtca ggccgtctcg atgaatgcgg
     4741 acctgcacca catcgcggaa cgtgctcgtc gacatcaccg tcgtccaggt gtgccgaagc
     4801 tggagtcgca cgaccgatgt ttcaagcgtc gtctgcgtcg ggcctgctgg tggcgtgctt
     4861 ttgggcggac tcgcgatcag cttgtgcggc aaagacagcg ccgccccgca gagcgcgagc
     4921 acggtccgcc tcttcatttg ccgccccctg cggcgttcgg cgttccagcg ggaagcgggc
     4981 tcgcaggcgg ctgtgcagcc ggcgtgggtg agggcgaagg agccgcggca ggctttacgg
     5041 tcagccccgg caaaagcggc agcacttctt tgtattccgg catttgggca agcttttgct
     5101 cgtattccgg gtacttgcgc ttcaccacct gcttttgcac gccaacgatg gtgtgatacg
     5161 ggattttttt cgatacggtg ttgatgatgc tcgtcggtag cgatccaccg gggtcggcat
     5221 gcacttggta ttcaacccgc gtccacttgc cgccgtttga tggccacaga aaccagtgac
     5281 cacagaggtg cggcatccga acgacgcctt tttgtggtgg ccaggtcgga tgctcgacgg
     5341 agttcatctc gatcttgacc atcttggcct tcatgtcgac gctggtgacg gcgcggatga
     5401 ccacgtcgcg atccgaaacc ggccacggcg ctccggtgcg gctgtaaaac gtctggtcgc
     5461 gatcgccgct gcggctgatc agtgtctggg cgacggcttc ggccatccac tccaggcgat
     5521 gatcgacatc atcgagcacg ccgagcaccg ccgtcaccgg ggcttctatc atgccggtgc
     5581 cttgaaactc gtgtagcggg gaattgccga cggtgcgccg atacacctcg atgccgccgt
     5641 cgttgcgcac cagttcccac tgtccggttg ggccgtgctc aaacgcgctg gtcgtcgaaa
     5701 ttcccccgag cgtaagcgca agcagcgcaa gaattcgacc tgggcgcttg aaatcggaag
     5761 cgagtgcaga ccttgtctga cggtgcatga acatctccag ttgcagaaaa tggaagctta
     5821 gaccagcagg ttgacgccgg tagaagcgag cttgagaact tcgtcggggc gcttcggcag
     5881 gtcggcggtc agcacttcgt gtccggtggt cgtgatcagg atgtcgtctt cgatgcggat
     5941 gccgatgccg cgccactcgg gaccgagcgg atcgtcgggc tgtacgtaaa gccccggttc
     6001 aatcgtaaac accgtaccgg gcaacagttg acgcgcttct ccatcgggaa aatagcgtcc
     6061 gacatcatgc acatcgagcc ccaaaaaatg gcttgagcgg tgcggataga aacgcttgag
     6121 cgcgccactt tcgacggcac tggctggatc tccgctcaaa agcccgagcg cacacagtcc
     6181 cgatgccaag cgatgacaga tcagggtgtg tagttcttcg acggtgacgc cgggccttac
     6241 cgctgcaatt gctgcgagct gtgcgtcgag cacgatgtca taaagctgcc gctgttcgcg
     6301 cgaaaactgt gcccgatgac ctggtgccgc gatcggataa gtccgcgtca catcgccggt
     6361 aaatccatcg agttcacagc ccgcatcgac gagcagaagc tgccccggtt cgagctgatc
     6421 ttggttgtcg acatagtgca gcaccgtcgc attttttccc gcggcgatga tgctctggta
     6481 gccaggtagg ccatttcgtc gtcggaaacc gtagtcgacc agcgcttcaa tttcgtattc
     6541 gcgcttgccg gcgctgcagg cgcgcatcgc caaaaggtgc gcttcgaccg tcacggcgca
     6601 cgaccggcgc atgctgagga tggcggcttc atctttgacg agccgcattt catgcagggt
     6661 gtttttcaga tcgacgatgc ggctcggggc gcggctgccg ttgcgttcgt tgcggcgcag
     6721 tcgcgcaatc gtcttgccga tcagctcgtc gtgctcggga cgatcgccga tcaagtagtg
     6781 gacttcgtcg cagccgtcca aaagttcgag ccgccgacgc tcaagttcgg aaatcgcaaa
     6841 cgactggtcg gcgccgtaca gctcggtcgc accctcgagc ccggcgcgat gtccgtacca
     6901 aagctcggcg aacttgtcgc gcgggcgaca caaaagggtg aagcggcgat ccttgccggg
     6961 ggtgaacacc gcgacggact cgggttccgt catgccggtc agatacaaaa agtccgacga
     7021 ctggcggaat cggtggtggt tgtcggcgtt gcggcgctcc tcgcggcccg aaaacagcac
     7081 agcgcaggcg tttttgggca gccgctctag cagagttgct cgtcgagagg cacaagctgc
     7141 aagcatgatg atgtaccagg ctaccaaaag tcggtcaggt tgcgactgcc aatcccggcg
     7201 ctactgcggg gacaaaatga cgccacctcg cgcaggcacg ctcaccgaca gtcgcccacc
     7261 cggcgctggc gcggtatacg tttgcccgct gagctgatct ttgagcgtcg atccgggggc
     7321 aaagcctagc tcgctcggaa tcggcacctg cgtgctcgtc ggatagctta gtcgactgag
     7381 caccacgagc gcgctctgtt tcgcatcgtc gtcgggccgt ccgtaggcgt agacatcttc
     7441 ggtcagcagg actgtcagca gttttcctcg tcgcaggccg cgaagacttt gccgggccga
     7501 aagcagacgt gtcatccgct ccagctgttt tttttctttt ggcgaaagct gcgcgtcgat
     7561 tcgcagacgg tgccgattgt ccggatcgcc gccgccaaac tcgccatatt cgtcgccgta
     7621 gtaaagcagc ggcattcccg gcatcgtgaa aagcgccagc gccccgagcc attgccggtc
     7681 gtaaggttca tcgtccaggg gagcctgcgg cagcttgtcc catttgttgt tggcgatgtt
     7741 gcggtcaaag gccgatccgg gcgtcgggtc gcgatacgtg cacatcgtcg caaaccgact
     7801 ggtatcgtgg ctgccgaggt aatttaccat cgtgctgcct ttgaactgat cgaggctggc
     7861 gtgggtccag taatcgaggt gcagaaaccg tttttcgtcg taagcgaaga cgcggtacgc
     7921 gctcgagtga taccaaacga agtcgaactg tccgtcgagc ccgtttggtc ccatgtagcg
     7981 cttgatggtg tcgtattcgc tctggttcgt cgcgacgtcg ccctcgcgcc agcccatcgc
     8041 cgtctcgccg agcaaatagt agcgcgtccc cgcttgctcg aatcgctcac gcacgcgcgc
     8101 cgtcaggttg aaaatcgccg catcctcgac gtgcttgacc gcatcgacgc gcagtccatc
     8161 gaggttaaag tgttcaagcc accacagcgt gtcggaaata aactgctctg atgcgtcggt
     8221 gacggtccag tcgatgtctg gcatgtacga cgcgaacagg cacgacaggc gtttttccgt
     8281 ccagtcgcag cccggtgatc cgcaaatgca gccggttcgg aaccagcttc gcttttgggg
     8341 atcttgaaaa tactcgtgct gctcgtggac gtggtttagc accgcatcca tcagcacccg
     8401 gatgccgtga cggtgcgccg cttgtaccag cttttgcagc gcatcgtcgc cgccgatgcg
     8461 atcgtctact ttgcgcgcca gcgtcggcca atagccgtga tacgcagcga cgcgatattt
     8521 gccgctttga tcgaggtgtg ccgtcgctgg ctgcatcgcg aatggactta accacagcgc
     8581 ttttacgccg agcttgtcaa agtagccagc ctcgatctgc tgcgtcacgc ccatcagatc
     8641 gccgccgcgg aaattcgctt ccggtaatac cccgccaatt tgaccaggat tgataccgtc
     8701 accgtcgaca aaacgatccg tcatcgccat atacagcggc gtatcggaaa agcggaacgg
     8761 ctcaggttcg atccacagcg gcaatagcag cgactccccg ttttttccac ccgccgacgg
     8821 agtcagcgag accagatact tgccgtcggc cagatcgcgc agatgcagct cggcgctttg
     8881 ccgatccggc gcaatcttga gttcgagcga tgacagcgat ggcaaagatc ctgggacaat
     8941 cgtgtccggt ttgcgtatct gcgactgaat ctggcaaacc cccggcgtca tcgcgtggga
     9001 cggggactgg actttcagct tggcaatgac ttcgccgtgt cctgcgctcg tgcgggtgat
     9061 ttgcagcgat cctggctcga cggaaagctt cggtacaaag cagttggcga cgcgcaggcc
     9121 cgagttttcg gtcccgtcga cgtacttgcg ataggcattc tgcgcatcga gcacccagct
     9181 tgcgccgtcc tcgaccagct tgtaaccgta gacgccgggc ggcaggctga tgctgatcgt
     9241 gtacgcaccg cttccgtcgg ggccgctcag cgtctgcgca ctcgtgctcc agccgttcca
     9301 ttcgccggca atcgcgacgg aacgaacgga cttggcggga acgtaacgca cggtcacggg
     9361 gcaggttgcg ccggctgctg gttcagcgag tgttacacac gggccaccct cgggcggttt
     9421 gtcacccagg tcggtgttgt cgcctggatc ggtgttgctg tttggcgtgc tgcaggccaa
     9481 cgcagatgcg gtcagaatta gcagggtcca gcgacggaac agatgtgaca tcggcaagct
     9541 cctgggcggg gaaaaccggg cactacttgg gggccgcttg ggacggcggc gtttcttttg
     9601 gcacaggccg actggggatt gcacccggct gattcggcgt cggggcgggc gctggtccgg
     9661 gttgcgtatt ttgctgcgtt ttttccggct gcgcatagcc ccagtcccgc atcaccgtcg
     9721 ctacttcgct ctgcgttgag ccgccaccac gcgacaagct ggtcgcaatc gcaccgggac
     9781 ttggctggtc gcgccacaaa agcacgtcgc cgcgttcgcc caaaacgggt ggataggccg
     9841 caaacagccg tcccaaaatc gtgccttgca gcgtcagggt caggggttct gagctgtccg
     9901 gcggtttggc atcgggcgtt ccttcatgac ccgctgcaac cggctgtggt gtcgtgcctt
     9961 gcgaaagcag cggcaggctg tacggtccaa gcagcagctg ttctttggcc agcggcagac
    10021 catcgagcgt cagttgcaaa tccagcttgg cgtcggggtc catgtgcaca tcaagctggc
    10081 cacctggctg cagcagcact tcaagctgac cgggccgcag gggggtcact tcgccgtcgc
    10141 ggacgcccgc caccgcaagt tgcaagtcac tgctcatcac caggccatgg agctgccggc
    10201 tcgtcggacc gggggccagt gcaaggtgcg tcaccggcat cttggcgtcg ggcagccgcg
    10261 gcgcgtgacg cgaaaactgc tctcgcatgg tgcgcagcgt ctgttcgaca agcggcgact
    10321 tcggtccttg cagcaggttt ctgtgctgct gtggatcgtc gcgtaggtcg taaagctcct
    10381 cgggaacttg aaacagcgca ctcgctgcct cgagaggctt tagccgcgga tcgctccgtc
    10441 gcagatacaa ataaccaaga gagcgcagcg cgcgcacatt ctgaccttcg acaaaggccg
    10501 cccgctccgt cgattcggct tcaccctgca tcagcggcaa caggctctgg ccttgctgaa
    10561 gatttccact cggcagcgac gcccgttttt cccacagacc aagcacctct aacaccgtcg
    10621 gggctacgtc gaacaggcgg acctggtttt tgaccacgac cccctgcggc agccgccccg
    10681 gcatcgccaa aatcatgggc acccgcaaaa tctcgtcgta ggccgaccag ccgtggtggt
    10741 acagcgtcgg ctgtttgagc gcgaggacga agtggttgtg gtttatgtcg aacacttcgc
    10801 cgtgatcacc gacgatgaca accatcgtgc gtgacgaaag cgcaagccga tcaagctcgg
    10861 ccagcacttg cgcgatttgc gcatcggcgt aggccgcttc cgccagatac gatcgcgccg
    10921 acggttccga tgcgaccggc tgcgggctgt tgcctcgtcc cgccatgtag gccgcctgtt
    10981 ccgtcgacgc tttgggcaga aactgtggcg gcggcgaata cggccagtgc ggcgcgtcgt
    11041 agtgcagctg caaaaagaac gaacgctcgc ggttttcttg cagatagcgc agcgccgccc
    11101 gggtgatcgc cggtgagtcg gccaccggat gtcgcacgtc ggccacttcg tcgaagccga
    11161 gcgacagacc gatttgcgga tagccgagca aaaagaaatt gtttccaatc gccgtcacct
    11221 tcacgcctga ctctcgcagg acgcgcggca gcagcttgcg atcgagcgcg tagaagcgct
    11281 gtcgatcgcg ctctttggga atcatgtcct cggcgttttg tccgaccgcc gacggcaaat
    11341 caccaccgag cagcgacagc agcgctggac gggtccacgt cgccgatgta atcgcttgct
    11401 caaaccgcgc gcctttttgc gcaaactttt gcacttcggg catcgccgaa accgcatccg
    11461 cgcggagcgt gtcgacgatg atcagcagca agttgggcgc cgcggcccct gcgcctttgg
    11521 cccacagctc gggattgcca atcactggaa ttccgacggg gcttccgtca ctgcgaccgc
    11581 acagctttac atcgtgctgg gtgtttgccg attctccgac gggggctgtg atggacactt
    11641 catggcgatc ccatcgactg gcggtcgtct gtttttcgct ccagactgga cggtcgtcga
    11701 tgcttagcga aagttcgacg ggcgctttgc cgttgtcggt tttctttggc agcagcgccg
    11761 agtgaaacac cagcgttccc aaggttggca gcgcgagtcg gaaacggtaa cagcttgccg
    11821 tcggcaaaag cagcgacagc ttggactcgt agacgccgcg atagcgattc cacaccggat
    11881 caaagcgcgg cggcgccgtc gtttccttcg ctggctcagg atacgtttcg acgttgggaa
    11941 tgccgatggc gacttcgagc acgcgcagcg ccgtatcgcc ccgcagtccg ctgtacggac
    12001 tgaccagctt gtagggcgtt tccaggctcg ccaaaatcga cgaaagcccc ggctgcggac
    12061 ccgtcggaat cagctcggct agacccgcca aatccgtcag tcgagcccac agcttgtcac
    12121 cgggcggcgg cgcaattggc ggcagcctcg gtgcctcttg tctgggcctt ggcctgacct
    12181 cgcgttccgg ttccggcccg gtgagcgtcg acggcgctgg cgtattttgc gtacacgccg
    12241 aatgtgcaat cagcagccat agcaaaaagt gcggcacgcg ccgcttcccc atggctgttt
    12301 cagtctgact acgaaccgac gatctcacga cggcgaattg ttccacagat ttgggaagct
    12361 agcaccacta catcttcgca aggccgctcg aatacgaaaa ctcggcgggg cttgcgccgc
    12421 cacctgtgcc gtgacggtag acgagcttgt acgaaaaggt cgaaaaactg cccggcagat
    12481 ccacgtcgac ttgccagcgc tcgaagtcct ggtcgatgtc cgttttccag tacagggtgt
    12541 ttttgctgcc cgcggcgccc atgggaatct cttggctcgt cgcccatccg tctgtcgaat
    12601 agaccaattt gagcactttg tcgtagtcga gatcctgcac cacaaacgac agt
//
LOCUS       DAORGN010000146        12629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2074_length_12629_cov_8.151265, whole genome shotgun sequence.
ACCESSION   DAORGN010000146 DAORGN010000000
VERSION     DAORGN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_12629_cov_8.151265"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..499
                     /locus_tag="PKL17_15030"
     CDS             68..499
                     /locus_tag="PKL17_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46097.1"
                     /translation="MSALMARRYYVRGCTELRDNELDDACQDLQAAVQLIPSYYEARI
                     AYATALLRLKDPPRAIQTLRAGLHYTTTGPGRALLCRALGDALVAGGDFLAAEQAFSE
                     SQAASPQASADLHDRLARLRARTGRFDEAFTELLAAARLSK"
     gene            complement(503..1678)
                     /locus_tag="PKL17_15035"
     CDS             complement(503..1678)
                     /locus_tag="PKL17_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46098.1"
                     /translation="MRYAATGRLFQTALLLGFSIGITGCEADLRGGDIDVGGGKNPVD
                     PGVDSCKVGETRCVSGAIQKCSSGKFVNDKLCAVGQICDPLRGCLDCSPVLPTTCVGD
                     TVHVCNPSGTIGEEKETCQPMGCRGGKCSSVCSPGSELIYVVDSSNRLLSFNPRDGKY
                     EYKLIGTLNCPAGSSIGGGGKATPFSMAVDRTARAWVLYSSGEIFWVNTTDASCKSSG
                     YAKLQSGFETFGMGFVSDAEGSEKESLFVAGGSYTNQRVADLASIDAGTLQLKKIAAL
                     QLSGQNSPELTGTGKGELYGYYPGAAPFVGQIDKTNAGSLRRWSLSSMGGTPSGWAFA
                     HWGGRFYIFITAQVGAAEKSMVWEFDPATGKATEVKTNASYKIVGAGVSTCAPVVIG"
     gene            complement(1764..2540)
                     /locus_tag="PKL17_15040"
     CDS             complement(1764..2540)
                     /locus_tag="PKL17_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNK46099.1"
                     /translation="MRLIPYALSDVGRKRAHNEDNFLVAENLGLFAVADGMGGHQAGE
                     MASRLAIEKLREQVTLPKQNATRSENLLSLTRAMMTVGADIFDAAQADPDLEGMGTTL
                     TALWVQGRRAYIAHVGDSRAYLYRDGKVQQLTFDHSWVAEQVRAGFLTEQEARESRFR
                     HIITRSVGFERDVQVDGAMLPVRVGDCLLLCSDGLSNFVEEPDLIEVLSRQLYRDVPQ
                     VLVDLANARGGDDNITVVLVHIANDQGPEGRLTLPAESPA"
     gene            complement(2586..4637)
                     /locus_tag="PKL17_15045"
     CDS             complement(2586..4637)
                     /locus_tag="PKL17_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNK46100.1"
                     /translation="MADLQQRGPTQVARKRRKVWISAMLLSTVGHLLVMPALLQRLAE
                     RPEPQLELTYLDPDVPTEGPAQDGPTAETPPSLPTPPSIAKAPEPKEKKPETPPDEKV
                     AERREKKPEPEKPKPENKPLDVAVLPHLKMVDQDQFPDEDDNKDARFLAQKNHSAAVD
                     TQASARNLIQQLAGETQASAPSENQLPQIGQAQQKAAELQDRPGDPKHLPQLAPPPTP
                     VARNPNLAVAQSAQASDKQKTAEAQSLGPEKNPGGETQLGDGSQLFKQGGGSESRESQ
                     GKFDRAEGLAGAPGLSPSRLRYQDYDRIVGYDVAESERRAAARAEQSQGIGRWDRIMA
                     KQALFRSALENFTPNVRVGNQSELGTRAHPFAAYIAQVHRQIHRFWGDGFLADLDRKT
                     GGDIYPRQLVTALEISIKPDGTLAQVVIAKTSGVMPFDAAAIDAVGSSAPFGEPPSSI
                     KSRDGNVYITWHFHRDERQCATDFVNAHILTTPPKSGPNVASSGVVPKNKPIFAAAGR
                     SKEEVRIPSEAREGSGRFESGRPDGHPSGLSRPSLGHGTGIDASEPSAPSKATALTKV
                     PDEAKGAAERWLDAMEHHDAKRMSAGSALPFTSGGKTVASDGPGLRSFFDEMMSEGVP
                     RRDRLHFYTLPEVKTRLGRAPRGSDGDEVAFAWVEQGGEDLILLLEPTDKGWKVVGLE
                     R"
     gene            complement(4823..5926)
                     /locus_tag="PKL17_15050"
     CDS             complement(4823..5926)
                     /locus_tag="PKL17_15050"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNK46101.1"
                     /translation="MSESQSPKVSLPKVTVGGKEIDLSDISSLVVKKDLDQPDYVQIS
                     LSNLGDTSGGSKFSAILKPGTDLVVKIKVEGEGEQELFKGFVHGDDPTYDAHSPVSTD
                     LQGMNAMHKLTRGRKTRTFTNQTDQQIIQKVVSEAGLSVNFGREPPTLRHEHLHQNNM
                     TDLEFIRMRAARTGRVIFCEDKTLYYTKREKDQGPVAKLDYNTQGTGALENFKPKMSA
                     AAQVKKVVCHGWDPFQSTRESQKMECKAEGGASPLGGESGSSAFGDNPEVHVFDIPFR
                     SKEEGDLLAKSILEERQMNFINAEATGLGNSQIKPGKVIELQLGGKESRFNGKYYVVG
                     CEFSYSHSSSGMGEGSGMGGFKGKYKLQRDAGM"
     gene            complement(5929..6384)
                     /locus_tag="PKL17_15055"
     CDS             complement(5929..6384)
                     /locus_tag="PKL17_15055"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46102.1"
                     /translation="MAELEKAELHDCDRGGKLTVLFNPKEFTVEKQVQWTQKEGAMTD
                     EPPEEFTKPTPANLTVTLHFDTYEQKSSVMEYTSKVEKLALIISKEKPRPPLCKFVWG
                     KFVFQGVVESVQQKFTMFLRDGTPVRCEATVKMKKASSAATGKQGGAGG"
     gene            complement(6377..7213)
                     /locus_tag="PKL17_15060"
     CDS             complement(6377..7213)
                     /locus_tag="PKL17_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46103.1"
                     /translation="MSDSQRNSTAPDFQPLGTSASLRMLGSVMGRIQPGSSSRGAGAA
                     RSETGEIPGGAAEKSAAAARGAAEPQSVLDAGWTPSVARAGMQVQQRQEAAGPLWSSL
                     PKADLKPVVSAVEADLGTYLAAQRETSGRGRAGSPELALLAGQSDAASIRASSRGTTT
                     GSGSDVQQAAVDISTRLLDAVRSHAAAQAVASDSRISLGDLTLIAFADSKRQMAAATS
                     HAEHTPDPHPLHKAIDNSAHKKFMEDRKGFDNKLKKVAELAIESLEKSKRIAKERFGS
                     HG"
     gene            complement(7216..7653)
                     /locus_tag="PKL17_15065"
     CDS             complement(7216..7653)
                     /locus_tag="PKL17_15065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK46104.1"
                     /translation="MSRREALPVYYFTVEIKGIQQGYFRSCSGLKSESEVVTIQEGGV
                     NTTELKLMGHNKYSNIVLKQGFVDAQLWALRQGYVNDEPGTKMQRIDGLIVHYGPGGK
                     RLHKWRFRKAWICKWEGPEFDATKNEISVESIEIAHEELTYEG"
     gene            complement(7673..9445)
                     /locus_tag="PKL17_15070"
     CDS             complement(7673..9445)
                     /locus_tag="PKL17_15070"
                     /inference="COORDINATES: protein motif:HMM:NF016845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNK46105.1"
                     /translation="MAPREIRPPGVYPLGAGARYTSLTIADTHIAGFVGIANRGPLDV
                     PILLQSWAEFAEVYGSTSEGYLARAVEGFFLNGGKSCYVVRVARRPRPGERLEPYHAL
                     QAERVLRDSWDKPTLRVLALNEGRWGNSVWVKTTRSTGAKTLLTLDLPVGAGQARVTS
                     TRGIEKGALLRIFDRDNSDYIVVTDIDEKNKTLSWGPETPVVRRYAAAAPTYVEVIEF
                     EIYASLRDRREVFKGLQLHPLSRRYAPRIINEESYLIRLEDLGSTSPWPNNLPVEASL
                     DKLTGGRDGIDGITAEDFIGYDNGLDDRRGLMTLEFVDEVGVISVPDAMLAYKSMSVP
                     EARSYVQHIHDAMIGICERSQDRFAILDAPDTRDIEEVRRMRQRLNTSFAAIYFPWVV
                     VAGSNGSQHKVPPSGFVAGVYARTEQSSGVHKAPANEQILGLTQLTLPLSDDHLGQLN
                     SEGINTIRAMTGRGIRIWGARSCSESDDWRYLNVRRLFIMLRRAIEKGTQWISFEPNT
                     METWSLIEREVSSFLSDLYIKGYFAGNSPEEAFIVRCNEETNSQETVDSGRLVTEIRV
                     APAIPAEFIYFTIEQQMGERVEPR"
     gene            complement(9911..10300)
                     /locus_tag="PKL17_15075"
     CDS             complement(9911..10300)
                     /locus_tag="PKL17_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HNK46106.1"
                     /translation="MAFKTEFPFTLPKGYIDSEGKLHRNGVMRLATAKDEIVPLQDYR
                     VQSNRAYLVIVLLSRVISKLGELAVVDTEVIENLFSTDLAYLQEFYRRINEEGAPRVH
                     AKCPSCQHEFDIDLLGGGDGRVVGQGG"
     gene            complement(10319..11251)
                     /locus_tag="PKL17_15080"
     CDS             complement(10319..11251)
                     /locus_tag="PKL17_15080"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK46107.1"
                     /translation="MTANARSYMNSRFFLELEQHPSGWLFSADGGHVQTESVNERMAG
                     GYPVRKHAGNVHVDEVTIACGTGMTQQFYSWIKKSMDYEHERHDGAIVHANYDFKEIS
                     RQTFQRALITEVGLPALDATSKEPAKMTIKFSPEMCTLEYSKGTRSINPVPLDPPKQK
                     LWTPANFRVRIAGLEDACKRISKVEAISIKQNVVENSIGEKLIFDKEPAQIDFPNVVI
                     TIPENDAEKWYKWHKEFVVEGKSYSTSEKHGSIEYLSSDLASTLFTLDLENLGIIKYT
                     PDRAESGGEKIRNIKIEMYCEKMKFEYKSNATYK"
     gene            complement(11404..12366)
                     /locus_tag="PKL17_15085"
     CDS             complement(11404..12366)
                     /locus_tag="PKL17_15085"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNK46108.1"
                     /translation="MAITKQSQGRAYTGGKYNLEFDPGLNAGWIRDAEGGHAVADVVT
                     EKMGADVLQRKHLGNVKYEEISFKCGTGMSKDFYDWIDTAIKFTNNTNGRKDGAIVHF
                     DYDYNELSRLTFFQGLVTEFGMPALDASSKDPAQMSIKFKPEWTRKTFSGGGKKSSTN
                     VKPDVQKKWLPSNFRLRFTNDSSFDTVLAKVNKIEALTVKQKVVENAIGELRDYQQEP
                     ASIEFPNLVITFAESHADPFYRWHEQFVIKGVNGDDQEKTALLEYLTPDLSTVLFTLT
                     LEHCGVFKLSPDKVEAGVESIRRVKAEMYCEEIKFNYTGAAIWK"
     gene            complement(12439..>12629)
                     /locus_tag="PKL17_15090"
     CDS             complement(12439..>12629)
                     /locus_tag="PKL17_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014181558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HNK46109.1"
                     /translation="QGALMGETPEKAFFVKCDEETNPPEVIDVGQLIVEIGLAPVKPA
                     EFVIFRIGQMPAGGGVQE"
BASE COUNT         2459 a         3891 c         3454 g         2825 t
ORIGIN      
        1 cccaaaaggc gcagacggaa aaaaatgaca gcccaggcgg tcgcttcggc ctgctggaga
       61 tcgactaatg agcgcgctga tggctcgtcg gtattacgtg cgcggctgca ccgagctgcg
      121 cgacaacgaa ctcgacgatg cgtgccagga tctgcaagcg gcggtgcagc tgattccttc
      181 gtactatgaa gcgcgcatcg cctatgcgac ggcgcttctg cggctcaaag acccaccgcg
      241 ggcgattcag acgctgcgcg ccggacttca ctacacgaca acggggcccg gtcgcgccct
      301 tttgtgccgt gcgctcggtg atgcgctggt cgctggtggc gatttcttgg ctgccgaaca
      361 agcgttttcc gaatcgcaag cagccagccc acaagcgagc gccgacctgc acgatcgcct
      421 ggcccgcttg cgcgcccgca ccggacgttt cgacgaagcg ttcaccgaac ttctcgccgc
      481 cgcccgtctg agcaaataac cgctagccga tcaccaccgg cgcacacgtc gaaacgcccg
      541 caccgacaat tttgtagctg gcgtttgtct tcacttcggt ggcttttccc gtcgctggat
      601 caaactccca gaccatcgac ttttccgctg ctccaacctg ggcggtgatg aaaatgtaaa
      661 agcgaccgcc ccagtgggca aatgcccagc ccgacggtgt gccacccatt gacgacaagc
      721 tccagcgacg cagcgacccg gcgttggttt tgtcgatctg tccgacaaac ggtgccgccc
      781 ccggatagta gccgtatagc tcgcctttgc ctgtgccggt cagctcgggg ctgttctgtc
      841 cagatagctg tagcgcggcg atttttttga gctgaagcgt gcccgcatcg atcgacgcca
      901 gatcggcgac gcgctggttt gtgtaggaac cacccgcgac gaacaggctc tctttttcgc
      961 tgccttcagc gtcggacaca aagcccattc cgaacgtctc aaagccgctt tgcagcttgg
     1021 cgtagccgct cgatttgcac gaagcgtccg tcgtgttgac ccaaaatatc tcgccgctcg
     1081 aatacagcac ccaggcacga gcggtgcgat cgacggccat cgaaaacggg gtcgctttgc
     1141 cgccgccacc gattgaactg ccggctggac agttcagggt gccgatcagc ttgtattcgt
     1201 acttgccgtc acgaggattg aagctcagca gccgattgct cgaatcgacg acgtagataa
     1261 gctcggaacc gggactgcac accgacgaac atttgccacc acggcagccc atcggctggc
     1321 aggtttcttt ttcttcgccg atggtgccgc tcggattgca gacgtgaacg gtgtcgccca
     1381 cacacgtcgt cggcaaaacc ggagaacagt ccaagcagcc gcgcagcgga tcgcagattt
     1441 gaccaacagc acacagcttg tcgtttacaa acttgccgct ggaacatttt tggatggcgc
     1501 cgctcacgca gcgggtttcg ccaaccttgc agctatcgac gcccggatcg acgggatttt
     1561 tgccaccacc gacgtcgatg tcaccgccac gaagatcggc ctcgcaaccg gtgatgccga
     1621 tcgaaaagcc gagcaaaagt gccgtctgaa acaacctgcc cgtcgctgcg taccgcatgg
     1681 gtgcctccca cgatgtgacg tttgggtaaa agacgtcgca tcgtacaaag cggccctgtc
     1741 agtcacaagc gcggtgtgat cacttacgcg gggctttcgg caggcagggt caggcggcct
     1801 tctggacctt gatcgtttgc gatgtgtacc agcaccacag tgatgttgtc gtcgccgccg
     1861 cgggcattgg caagatcgac cagcacctgc ggcacatcgc gatacagctg acggcttagc
     1921 acttcgatca gatcgggttc ttcgacaaag ttcgacagtc cgtcggaaca caaaagcaag
     1981 caatcaccga cgcgcaccgg cagcatggca ccgtcgactt ggacatcgcg ttcaaagcca
     2041 accgatcggg tgatgatgtg acgaaaacgc gactcgcgcg cttcctgttc cgtcaggaaa
     2101 ccggcgcgca cctgttccgc cacccacgag tgatcaaacg taagctgctg cactttgccg
     2161 tcgcggtaca gatacgctcg ggagtcgccg acgtgcgcga tatacgcccg tcgaccctgg
     2221 acccacagcg ccgtcagcgt cgtgcccatg ccttcgagat cgggatcggc ttgcgccgca
     2281 tcgaaaatgt ccgccccgac cgtcatcatc gcccgcgtca gcgacagcaa gttttcactg
     2341 cgcgtcgcat tctgtttggg cagcgtgacc tgctcgcgca gtttttcgat cgccagacgg
     2401 ctcgccatct cgccggcttg gtgcccgccc attccgtctg caaccgcaaa cagacccagg
     2461 ttttccgcga caagaaagtt gtcctcattg tgcgcgcgtt tgcggccaac gtcggacagt
     2521 gcatagggaa tgagtcgcat gctgtttgac caatatcacg aggtgctacg ttcggcgaac
     2581 ctcggctagc gctcgaggcc aacgactttc cagcctttgt cggtcggctc aagcagcaag
     2641 atcaaatctt cgccgccttg ctcgacccac gcaaacgcga cctcatcacc gtcggagccg
     2701 cgtggcgccc gaccaagccg cgtcttgacc tccggcagcg tgtagaagtg cagccgatcg
     2761 cgccgtggca cgccttcgga catcatttcg tcgaaaaacg atcgcagtcc ggggccgtcg
     2821 ctcgccaccg tcttgccacc cgacgtaaac ggcagcgcac tgcctgcgct catgcgcttg
     2881 gcgtcgtggt gttccatcgc gtccagccag cgctcagccg cgcctttggc ttcgtctggg
     2941 actttggtaa gtgcggtggc tttgctcgga gcagatggct cgctggcatc gattccagtg
     3001 ccgtgcccaa gcgacggacg cgacagaccg gacggatgac catcgggtcg acccgattca
     3061 aagcgaccgc tgccttcgcg cgcttcgctg ggaatgcgga cttcttcttt cgatcgacca
     3121 gccgccgcaa agatgggttt gttttttggc acaaccccgc tcgacgcgac gttcggtccg
     3181 gacttgggcg gcgtcgtcag gatatgcgca ttcacaaagt ccgtcgcaca ctgccgttcg
     3241 tcgcgatgaa aatgccaggt gatgtagaca ttgccgtcgc ggctcttgat cgaagacggc
     3301 ggttcaccaa acggtgccga gctgcccact gcatcgatcg ccgccgcatc aaatggcatc
     3361 acgccagacg tcttggcgat caccacctgc gccagcgtcc catcgggctt tatcgaaatc
     3421 tccagcgccg tcaccagctg gcgcggatag atgtcgcccc cggttttgcg atcgagatcg
     3481 gccaaaaatc cgtcgcccca aaagcggtga atctgccgat gcacttgcgc gatgtaggcc
     3541 gcaaatggat gcgcgcgcgt ccccagctcg ctctgattgc cgacgcgcac attcggcgtg
     3601 aagttttcca gcgccgatcg gaacagcgcc tgcttggcca tgatccgatc ccagcgtccg
     3661 atcccttgag actgctcagc gcgggccgcg gcacgacgtt ccgattcagc gacgtcatag
     3721 ccgacgatgc gatcatagtc ctgatagcgt agccgcgacg ggcttaaacc cggtgcacca
     3781 gccagacctt cagcgcgatc gaacttgccc tggctttcgc gtgactcgct cccgccgccc
     3841 tgtttgaaca gctgcgatcc gtcgccaagc tgggtctcgc caccgggatt tttttccggc
     3901 cccaggctct gcgcttccgc tgtcttttgc ttgtcgctcg cctgcgccga ttgtgccacc
     3961 gccaagttcg gattccgcgc caccggcgtc ggaggcggtg caagctgcgg cagatgtttg
     4021 ggatcgccag gccgatcttg gagttccgcc gctttctgct gcgcctgacc gatctgtgga
     4081 agctgatttt ccgacggagc actcgcctgc gtttcccccg caagctgctg gatcaggttg
     4141 cgcgcactgg cctgagtatc gaccgcagcg ctgtggtttt tctgggccag aaagcgcgcg
     4201 tctttgttgt cgtcttcatc gggaaactga tcctgatcga ccatcttgag gtgcggcagg
     4261 accgccacgt ccagcggctt attttccggc ttcggcttct cgggctctgg cttcttttct
     4321 ctccgctcgg ctactttctc gtcgggtggc gtctccggct tcttttcctt gggctccggc
     4381 gccttggcga tcgatggcgg ggtcggcaaa ctgggcggcg tctccgctgt tggcccgtcc
     4441 tgcgccggcc cctccgtggg cacatcgggg tccagatagg tcagctccag ctgcggctcc
     4501 ggtcgctcgg ccaaacgctg gagcagcgcc ggcatcacca acaggtgccc aacggtcgac
     4561 agcaacatcg cagaaatcca caccttgcga cgctttcgcg ccacttgggt gggcccacgc
     4621 tgttgcaaat cagccatcct cttaccctac caagggattg ggcggaccgg caaaccgtcc
     4681 gatggccaaa agcccgcagg tgcgccccga ccaggcacgg gcaccacgca gtcggaccga
     4741 cctacatcgg cccactcgga agcgattttt catggaaatc cgaagcgaag acacgcggga
     4801 ggttgccaaa gcccgacgag gatcacattc ctgcgtcgcg ctgaagtttg tatttgcctt
     4861 tgaagccgcc catgccggag ccttcgccca tacccgagct gctgtgtgaa taagaaaatt
     4921 cgcagccaac gacataatat ttgccgttaa agcgactttc ttttccacca agctgaagct
     4981 cgatgacttt gccgggcttg atctgcgagt tgcccagccc ggttgcttcc gcattgatga
     5041 aattcatctg gcgttcttcg agaatcgatt tcgcgagcaa atcaccttct tctttgctgc
     5101 ggaatggaat gtcgaagacg tgtacttctg gattatcacc aaaagcactg ctgccggatt
     5161 caccaccaag tggcgaagct ccaccttcgg ctttgcattc cattttctgc gattcacgag
     5221 tcgactggaa tgggtcccag ccgtggcaaa cgactttctt cacctgcgct gcagccgaca
     5281 ttttcggctt gaagttttcc aatgcgccag tgccttgcgt gttgtaatcc aacttagcaa
     5341 ctgggccttg gtctttttcg cgcttggtgt aatagagcgt tttgtcttca cagaaaatta
     5401 cacgaccggt tcgagcagcc cgcatccgga taaactcgag atcagtcatg ttgttctggt
     5461 gcaagtgctc gtgccgaagc gttggcggct cgcgaccaaa gtttaccgac aaaccagcct
     5521 cactcacgac tttttgaatg atttgctgat cagtttgatt ggtaaacgtc cgcgtcttgc
     5581 gaccgcgcgt cagcttgtgc atggcattca tgccttgcag atccgtcgac accggagagt
     5641 gggcgtcata cgttggatcg tcgccgtgca caaagccctt gaacagctcc tgttcgccct
     5701 caccctcgac cttgatcttc acgaccaagt cagttcctgg cttcagaatc gccgagaatt
     5761 tgctcccgcc gcttgtatca cccaggttcg aaagagagat ttgaacataa tcgggctggt
     5821 caaggtcctt tttgaccacc aggctcgaaa tgtcgctgag gtcgatctct ttaccgccta
     5881 cagtcacctt cggtaggctg accttgggac tctgcgattc cgacatgatt agcctccagc
     5941 gccgccttgt tttccggtag cggctgaact tgctttcttc attttgacag tcgcttcaca
     6001 gcgcacaggc gtgccatcac gaagaaacat ggtgaatttc tgttgaacag attccaccac
     6061 gccctgaaac acaaattttc cccatacgaa tttgcacaac ggcggacgcg gcttctcttt
     6121 gctgatgatc agcgcgagct tttccacctt ggaggtatac tccatcaccg agcttttttg
     6181 ctcataggtg tcaaagtgca gcgtgacggt caagttcgca ggcgtcggct tggtgaactc
     6241 ctcggggggc tcatctgtca tggcgccctc tttctgggtc cactggactt gcttttcgac
     6301 cgtaaactct ttggggttaa agagtactgt caatttgccg ccacggtcgc agtcgtggag
     6361 ttctgctttc tctagttcag ccatgtgatc cgaacctttc cttagcaatc cgcttggact
     6421 tttcgaggct ttcaatcgct agctcagcaa cctttttgag cttgttatcg aagcctttac
     6481 ggtcctccat aaactttttg tgtgcagagt tatcgattgc tttgtgcaat ggatgcggat
     6541 ctggcgtatg ttcggcatgc gatgtcgctg ccgccatttg ccgtttgcta tccgcaaatg
     6601 caataagcgt caaatcgccg agcgatatcc ggctgtcgct ggccaccgcc tgcgccgccg
     6661 catgcgatcg caccgcatcg agaagccgcg tcgatatatc gacggcagcc tgctgaacgt
     6721 ccgacccaga gcccgtcgtc gtgccgcgtg agctcgcgcg aatggacgcc gcatccgact
     6781 gaccagccag gagggccagc tctggactcc cggcgcgccc ccgtccgctt gtctctcgct
     6841 gggcggccag atacgttccc aaatcggctt cgaccgccga caccaccggc ttcagatccg
     6901 ctttcggcag actgctccac agcggtccgg ctgcttcctg acgctgctgc acctgcattc
     6961 cggcgcgggc gacagacggt gtccaacccg cgtcgagcac cgactgcggc tcggctgctc
     7021 cacgcgccgc cgccgcgctt ttttccgccg caccacccgg aatttcaccg gtttccgacc
     7081 gagccgcccc ggccccacgc gaagacgagc cgggctgaat ccgacccatc accgacccga
     7141 gcatccgcag cgaggccgag gtgcccagcg gctgaaagtc cggtgccgtt gaattgcgct
     7201 gtgagtccga catgtttaac cttcgtaggt caattcttcg tgagcaattt caatgctctc
     7261 aaccgagatt tcattcttgg ttgcgtcgaa ttcagggcct tcccatttgc aaatccaggc
     7321 tttgcgaaaa cgccacttat gtagccgctt accgcctgga ccataatgaa ctatcaggcc
     7381 gtcgatgcgc tgcatcttgg tgcctggttc atcattcaca tatccttgcc gcagagccca
     7441 cagctgtgca tcaacaaaac cctgcttgag cacaatgttt gaatatttat tgtgccccat
     7501 gagcttgagc tcagtggtat tgactccacc ttcctggatt gtgacgactt ccgattccga
     7561 cttgaggccg gagcatgatc ggaaatagcc ctgttgaatc cccttgatct cgacggtgaa
     7621 gtaataaaca ggcagtgcct cgcggcgtga catgttttag atacctctct gctcagcgcg
     7681 gttccacgcg ctcgcccatc tgttgttcaa tcgtaaagta aataaactca gcggggattg
     7741 ccggagcaac tcggatttcg gtgacgagac gaccgctatc gacagtctct tgcgagttcg
     7801 tctcttcgtt gcatcgaacg atgaacgcct cttccggtga gtttccagca aaatacccct
     7861 taatgtataa atcgcttaag aacgaagaca cttctcgttc aatcagcgac caagtttcca
     7921 tcgtatttgg ttcaaatgat atccactgcg ttcctttttc aattgcccgt cgcagcatga
     7981 tgaacaaccg acgcacattc agatatcgcc agtcatccga ctccgagcag ctgcgcgcgc
     8041 cccaaatgcg aattcctcgt cccgtcatcg cccgaatcgt gttgatgccc tcggagttga
     8101 gctgtccaag gtgatcatcg gacagcggca acgtaagttg cgtgagcccg aggatctgct
     8161 cgttggcagg cgccttgtga acgccgctcg actgttcggt gcgggcatag acaccagcga
     8221 cgaagcccga cggtggaacc ttgtgctgcg aaccgtttga accggcgacg accacccacg
     8281 gaaagtaaat ggcggcaaag ctggtattca gccgctgccg catccgacgg acttcttcga
     8341 tgtcgcgggt gtccggtgcg tcgagaatcg caaagcggtc ctgtgaacgc tcgcagatgc
     8401 cgatcattgc gtcgtggatg tgctggacgt agctgcgcgc ttccggcacc gacatgcttt
     8461 tgtaggcgag catggcgtcg gggaccgaga tcacgccgac ttcatcgacg aactccagcg
     8521 tcatcaggcc acgccgatcg tcgagtccat tgtcatagcc gataaagtct tcggcggtga
     8581 ttccatctat gccgtcgcgt ccgccggtca gcttgtcaag cgatgcttcc accggcaggt
     8641 tgttcggcca cggcgacgtc gatcccagat cctcgagccg gatcagatac gattcttcgt
     8701 tgatgatgcg cggcgcatag cgtcgagaca gcggatggag ctgtagcccc ttaaacacct
     8761 cacggcgatc gcgcagagac gcataaattt cgaattcgat gacttcgaca taggtcggcg
     8821 cggcggcagc gtagcgacgc accaccggcg tttccggccc ccacgacaga gtcttgttct
     8881 tttcgtcgat gtccgtgacg acgatgtagt cgctgttgtc gcgatcaaag atccgcagca
     8941 gcgcgccttt ttcgatgccg cgcgtcgacg tgacccgcgc ctggcctgca ccgacgggca
     9001 gatccagcgt caaaagcgtc ttggcgccag tgcttcgcgt cgtcttgacc cagacgctgt
     9061 tgccccagcg cccctcattg agcgccagga cgcgcagcgt cggtttgtcc cacgaatcgc
     9121 gcagcacccg ctcggcctgt agcgcatggt acggctccag cctctctccg gggcggggcc
     9181 gacgggcgac gcgaaccaca taacaggact tgccgccgtt taggaaaaag ccttccaccg
     9241 cccgcgccag atagccttcg ctcgtcgagc cgtagacttc cgcaaactct gcccacgact
     9301 gcagcaggat cggaacatcg agcggaccgc ggttggcaat cccgacgaag ccggcgatgt
     9361 gcgtgtcggc gatggtcagt gaggtatagc gcgcgcctgc accgagtgga taaacgccag
     9421 gtggtctgat ctctcggggt gccatgcaag ttcctttggc caaccaggtc tcctcggggg
     9481 gcccatcacc ccgtcgaaaa gacgcaacag aaagagaatt gtgcgcagac attttagccg
     9541 atttttcggc gtactaccta cgaaaaatgc gggcacgcac cacaggtttt tcgacggacc
     9601 gcgccaagtt ctgaccggat gttttgtgca ccgaccgata aactcggcgc ccaccggcga
     9661 tcgagttgtc ggccaaatgg aggagagaaa gcgcgttcgc agccttgatg cggcttgcgt
     9721 tccagtgtcg gggtgatttg tggctaaccg cgtttgcgag cgtcgtttat ctcgcggttg
     9781 attcgagcga tttcacgcaa ccagatgtgc cgctcgtcgt gttccatgtt gaggatgtcc
     9841 tcaacaggcc agtgaaagtg ataggcgata taggctacct cccggtacaa ctgctccagg
     9901 gggtagctcg ttatcccccc tggccgacaa cgcggccatc ccctcccccg agcaagtcga
     9961 tgtcaaactc gtgctggcac gaggggcact tcgcatgcac gcggggagcg ccttcttcgt
    10021 ttatgcgtcg atagaattct tgaagatacg caagatcggt cgaaaacagg ttttcgatga
    10081 cctcggtatc gaccaccgcc agctcgccaa gcttggagat cacgcggctc agaagcacga
    10141 tgaccaagta ggcgcggttt gactgaacgc gatagtcctg aagaggaaca atctcgtcct
    10201 ttgcggtagc aagccgcatc acaccattgc ggtgcagctt gccctcgcta tcgatatagc
    10261 ctttcgggag cgtgaatgga aactcagtct tgaatgccat cgctgtctct ctcagtgcct
    10321 atttgtaggt tgcgttcgac ttgtattcga acttcatctt ttcgcaatac atctcgatct
    10381 tgatgttgcg aatcttttca ccaccagact ctgcacgatc tggcgtgtat ttgatgatac
    10441 cgagattttc aagatcaagg gtgaacagcg tcgacgcgag atccgagctg agatactcga
    10501 tcgagccatg cttttcactc gtcgaatagc tcttgccctc gaccacgaac tctttgtgcc
    10561 acttgtacca cttttccgca tcattttcgg gaatggtgat gaccacgttc ggaaaatcga
    10621 tctgcgccgg ttccttgtcg aagattagct tttcgccaat cgaattctcg acgacgtttt
    10681 gcttgatgct gatcgcttcg actttggaaa tgcgcttaca cgcatcttcc agtcccgcta
    10741 ttctcacgcg gaagttcgca ggagtccaca gcttttgttt gggtgggtcg agaggtacgg
    10801 gatttatcga ccttgtaccc ttgctgtact ccaatgtgca catttctggt gaaaacttga
    10861 tggtcatctt cgctggctct ttgctggtgg catcaagcgc aggaagtccc acctccgtga
    10921 tcagtgcacg ttgaaatgtc tgtcgcgaaa tctctttgaa atcgtaattg gcatgaacaa
    10981 ttgcaccatc gtgccgttca tgctcataat ccatactctt ttttatccag ctataaaact
    11041 gctgggtcat accggttccg cacgcaatgg tgacttcgtc gacatggaca ttgccagcgt
    11101 gcttgcgaac cggatagcca ccagccatcc gctcattgac ggactcggtc tgcacatgcc
    11161 ctccgtcagc agaaaacagc catcccgacg gatgctgttc cagctccagg aagaatcgac
    11221 tgttcatgta gcttcgagca ttggcggtca ctggagtccc tcgttcaaat ataaaagtct
    11281 ttgccctgaa gcgggacagg cgactgagtc cccccagccg cctgtcccaa acccaaaacc
    11341 aactaaacac ctgcaagtcc ggcgcgattg ccggttagcg acttgcagac attgcgactg
    11401 gaattacttc cagatcgcag cgccggtgta gttgaacttg atctcttcgc agtacatttc
    11461 ggccttgacg cggcggatcg actcgacacc ggcttcgacc ttgtccggcg agagcttgaa
    11521 cacaccacag tgctcgagcg tcagggtgaa cagcacggtg ctgaggtccg gcgtcaggta
    11581 ctcgaggagc gccgtctttt cctgatcgtc gccgttgacg cccttgatca cgaactgctc
    11641 gtgccaacgg tagaacggat cagcgtgcga ctccgcgaag gtgatcacca ggttcgggaa
    11701 ctcgatcgaa gccggctcct gctggtagtc acgcagctcg ccgatcgcgt tctcgacgac
    11761 cttctgcttg acggtgagcg cttcgatctt gttgaccttg gcgagcacgg tgtcaaacga
    11821 cgagtcgttg gtgaagcgca ggcggaagtt cgagggcagc cacttcttct gcacgtccgg
    11881 cttgacgttg gtcgagctct tcttgccgcc gccgctgaag gtcttgcggg tccactcggg
    11941 cttgaacttg atcgacatct gggccggatc cttcgacgag gcatccagcg cgggcatacc
    12001 aaactcggtc accagaccct gaaagaaggt gagacggctc agctcgttgt aatcgtagtc
    12061 aaagtgaaca atcgcaccgt ccttgcggcc gttggtgttg ttcgtgaact tgatggccgt
    12121 gtcaatccag tcatagaagt ccttcgacat accagtgccg cacttgaacg agatttcctc
    12181 gtacttcacg ttgcccaagt gcttgcgctg caggacgtcc gcacccatct tctcagtcac
    12241 gacgtcggcg acggcatgac caccttcggc gtcacgaatc cagcccgcgt tcaggcccgg
    12301 atcgaactcg aggttgtatt tgccaccggt ataggcacgt ccctgagact gctttgtaat
    12361 cgccatcgaa ctcctcccaa tttgacgacg gggagcccta gctcactcga accagggcca
    12421 tccccgccct ttacagaact actcctggac gccgccgcca gcgggcatct ggccgatgcg
    12481 gaagatcacg aactcggccg gcttcactgg tgcgaggccg atttcgacga tgagctgacc
    12541 cacgtcgatg acctccggcg ggttggtttc ctcatcgcac ttgacgaaga acgccttttc
    12601 cggcgtctcg cccatcagtg cgccctggc
//
LOCUS       DAORGN010000147        12525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2099_length_12525_cov_6.138653, whole genome shotgun sequence.
ACCESSION   DAORGN010000147 DAORGN010000000
VERSION     DAORGN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2099_length_12525_cov_6.138653"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PKL17_15095"
     CDS             complement(<1..990)
                     /locus_tag="PKL17_15095"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNK46110.1"
                     /translation="MSAELPHSLSAQLQAATVRELLRAWHHFNATLFRRALMPPQIGL
                     TSSLSRLGLWVPATRSLLLSEALVREKPWGIVLEVLKHEMAHQYVHEILGRTDETAHG
                     SAFQAVCARMGIDHSAAGLPTSNETALDGERARQLTRIARLLSLAESQNVHEAESAMQ
                     EAQRLLLKYNLEIPTGDVRRQAFRHLGEPRGRIDEHLRLIAVVLGEYFFVETIWVSSF
                     DVRTGKSGSVLEVLGRPENLEMSAYVYDFLLHSAESLWQAHKAKLGISSNRERRTFLS
                     GVIIGFANRLREQKRTQQQQGLVWVADAELSKFLRQRHPNIRRVYSQGQPRSET"
     gene            1101..2105
                     /locus_tag="PKL17_15100"
     CDS             1101..2105
                     /locus_tag="PKL17_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46111.1"
                     /translation="MNPIAFVPSSRALFVSDAADQTGLAPVTFALFDRLRLQGYPTQV
                     RLLLPGSELAFRVASGVMVDLLDAMPILLSPDVQHEVLQSRELCGISKVLRLALSVVE
                     RGQAWPKLSRMGHGTRFSARWKAALSPSERAELCRIGKELSLCIGELLSTPLSSERWQ
                     HIGAGPGLSSLLLHATVDLVVREGCRRGAQVRLAECSTKTWEQRLVMALGDEKGLLLS
                     PLPSDETFAARTAELNDWAGLALSGSSRPLLPSPPSWQASESLQQLVSRFLRQAGQLS
                     AVLIAGKPAPRMLPMSAWRNEKPETERKPAVLPRKLLDEALQLLRDKRPGFADRHQAA
                     "
     gene            complement(2119..4545)
                     /gene="lon"
                     /locus_tag="PKL17_15105"
     CDS             complement(2119..4545)
                     /gene="lon"
                     /locus_tag="PKL17_15105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNK46112.1"
                     /translation="MQKPKGGTPDQSGPVEIPDVISILPLRNSVLFPGSIIPIDVGRR
                     KSVKLIEEAIAKEKPVIGILTQRDARTEDPGEGDMYAVGTAARILKVIKLAKDNFSVI
                     LQGVVRFRVQGYEGSDPFLNCRVTSIPDPTVADVELDALVMNLKDIAKRVIKLMPELP
                     KEASSLVESVTEAGQLADLITSQLDIPVEEKQDLLETFDLKQRMRKVLQFLTRQLEVL
                     KVREKINTQVQEEMGRNQREYVLRQQLKAIKEELGELDEGGGDAEEFKTKIIDAKMPP
                     DVEKVALKQFDRLKQMQPSSAEYTVTRTYLDWLVDMPWSVSTEDKIDIQEARNILNSD
                     HYDLEKVKKRILEYLAVLKLKNDKKGPILCLVGPPGVGKTSLGKSIAKAIGRKFVRVS
                     LGGVRDEAEIRGHRRTYVGSLPGRIIQGLKRAGTNNPVFVLDELDKLGHDFRGDPSSA
                     LLEVLDPEQNNTFSDHYLEVPFDLSKVMFIATANVLDPIPAALRDRLEVLEIPGYTRS
                     EKLQIAKNFLIPKQVDEHGLNGDNIAFEDAAVSEVIDSYTREAGVRTLERELGSVCRG
                     VAVKVAEGEAKEKTTIVADKIPDYLGPQKYLSEVAERTSIPGVATGLAWTPVGGDILF
                     IEATRMPGKSSLTLTGQLGDVMKESAQTATSYVRSRAKQLGIDEHFLDKADLHIHVPA
                     GAMPKDGPSAGITMFVALTSMLTGIPVRNDVAMTGEITLRGNVLPVGGIKEKLLAAHR
                     AGIRRAVLPERNRKDILDVPDEVKNDMEIFYATKMDEVLPLVLTGPVPTTVVPPPPAP
                     PAAESSPSTN"
     gene            4767..5753
                     /locus_tag="PKL17_15110"
     CDS             4767..5753
                     /locus_tag="PKL17_15110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK46113.1"
                     /translation="MRLVHYPCKNLPILAGECVVPAEIERIDRRTASAWAKGISVDDY
                     LRRERFLKTRPYAAGMTIHALLDDDGSDLQAQILASCETYRVPVVVPGRRTIGFGLGV
                     ASVFVDEPLRGHGFAATLIQHVHDHFRAQGAAMMYLMSEIGPTLYARLGYVTTPLSLR
                     RFPAAPLPAGLRVEPIHADELATLIPRLPPPRSPLYIPANREQLDWHFARGQFYAGLA
                     QGELPTEIGARSADAVAIWDVDHRPDCRRVRVLLVAAEKPSVHVEHVLCAAAAAAHAV
                     GLPFAEVWETAALSPFLDGGIKIEADDLPMLCPLAPDVSATDFLDVQRFHWL"
     gene            5812..7338
                     /locus_tag="PKL17_15115"
     CDS             5812..7338
                     /locus_tag="PKL17_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNK46114.1"
                     /translation="MRIPTRPTVIIRHCPDYDVQRIKQLFDEGLKTLGLRPTGRTLIK
                     PNIVAAGPLFANAHTRPEVAEGLILALQAMADDSVTEIAVGERCGITIPSRAAFQESG
                     FDDMLERTGAKRYLFDEVPQVEVRYSHEGRLRDYVFTPEPIAKADFFINLPKFKAHPW
                     TTVTFCLKNYIGIQDDRHRLIDHDHRLNEKIADLQFIVQPQFCAIDAIIGGEGRMLTP
                     KPFPLGLLIMANSQLALDAVCCHIVSLNPRSVAHLQLAEDRGFGTTDLSQIDVIGDVT
                     LDEAKRRASGFQVGLIRIEKYFEGSRITAYAGPPPEPEHSDYCWGGCPGALQEAIEVL
                     RLYDEQCDHKLPRLHVVFGAYRGPIPAQPGEKVIFIGDCADWEGELQGQLVQIRSVYE
                     DGRKRDPYQASHDDVFAKMAAVSMKMLWNEGTSHIRLRGCPVSVCEQVLAVVALSDVN
                     NPYLDMEHAVPFQKNYLAWRASQAMQRLRGIPYQKPGACSRGQAAPEVSPKHSEGGHG
                     "
     gene            7331..8374
                     /locus_tag="PKL17_15120"
     CDS             7331..8374
                     /locus_tag="PKL17_15120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK46115.1"
                     /translation="MDSPVQMEPPNRWQTLRDELDFWRFLIPRETVQLAGRHLPLEKL
                     RDLLPKALRERLRFGLYPARVSDPLAVICAREHPPWGIPAQLDPQKPLHFVDPPRVSI
                     LMVTYGNWDLTALCLASLQRLAGSIPFELIVVDNSSPDDTPQRLKELEQSALLPMRVV
                     LNRHNLGFAAANNQAARLARGDVLVLLNNDTIVTEDWLPRLLAVLDEDPQVAMVGPRT
                     NSCGNEAALGTPYATVADMFAFAKRYTDAHRGGRKEPDMLTLFCTAIRTSVWQEVGGL
                     DEGYGLGMFEDDDLTMAVRRCGHKVLLCEDAYVHHFGGAAFGKLPPRKYLRLFFHNRR
                     YFERKWHTEWKKR"
     gene            complement(8394..8987)
                     /locus_tag="PKL17_15125"
     CDS             complement(8394..8987)
                     /locus_tag="PKL17_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46116.1"
                     /translation="MGDIYEAIEQLSVVLSLRLENTVRKRGWAGRPIVERFEVRGPER
                     SVFPVRIAVTAPLSHSITFVPADQPSEDGQSWAIAVKRSDGVIRRSFSDGLTVRKVEN
                     GHQMFVRHEVLSEPKFRQVIEDLAGPSLAGHAVTIAKAFKSRFGSLSADVYEILFTAQ
                     HQEQRELMHEAVLTGINQLDVETELGRLSKWQPDDAK"
     gene            complement(9039..9245)
                     /locus_tag="PKL17_15130"
     CDS             complement(9039..9245)
                     /locus_tag="PKL17_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNK46117.1"
                     /translation="MAQRKELEITISPSGEVSVQVKCIPGQSCVEETKFLEDALGNEV
                     KDRQLTSDYYQQGNTGSTGIYNRG"
     gene            complement(9279..9674)
                     /locus_tag="PKL17_15135"
     CDS             complement(9279..9674)
                     /locus_tag="PKL17_15135"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNK46118.1"
                     /translation="MSHFTTVATKINNIACLLKALDKLKLKYTHSEQGVEVRGWRGQK
                     STAEVSINMGRYDIGVVKNEDGTYGLQADWWGVETTVGKTEQEVVDEINREYAYQTVV
                     VACEAQGYKIEEQNVAEDGTIKLSVSKWG"
     gene            complement(9690..11303)
                     /locus_tag="PKL17_15140"
     CDS             complement(9690..11303)
                     /locus_tag="PKL17_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46119.1"
                     /translation="MTQEQAAAPPTDSEELNRSEKEILDLIRARYSLMYIISSEEQRV
                     EDSLLKLARRRDMKLGSWSITRGFVQKFGTLKGGDAKDPIKALDYIAAAEGRGLFVLR
                     DFHAFVNDPTVVRKLRDLAHDLRKTQKNVFILSAVTKIPPELEKDLCIVDWDLPNRPE
                     LNKVVESLLHQLPQGCEPGIAKEAEGREQIVDAALGLTLTEAENVIAKSIVRHKTFDL
                     PTILAEKKHIIRKSGILEYFEAEEKLDGVGGLEVLKQWLVKRRHAFSSEARDFGLPLP
                     KGILLLGVPGCGKSLTAKAVGAAWQMPLLRLDVGKIFGGLVGASEENMRKALKTAEAV
                     APAILWLDEIEKGLSGTGSSNMSDGGTTSRVFGTFVTWMQEKTAPVFVIATANDVRSL
                     PPELLRKGRFDEIFFVDLPTQEERGEIIKIHLKKKGRSIQHLDLPKLIDAMAEFSGSE
                     VEQVVVSALYEAFDADPHNRELSSDLLLHSAREIVPLAVTMAEKIADMREWSRTRART
                     ASLPASQQPQRKTKDRFAVPSEPGGRIEL"
     gene            complement(11338..>12525)
                     /locus_tag="PKL17_15145"
     CDS             complement(11338..>12525)
                     /locus_tag="PKL17_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46120.1"
                     /translation="LRQDLKQLKEQFDKTKAQADELKTQSEAKDRELAAAKEDVRRSK
                     QAADNLTQKLQEMARSRDEQMRAINSQIGDVDKLRDILKERERLIEEQRVGLVNQESQ
                     LKDLRKRSEEQERELSSIRGERDNLRERQNRAQVQVEELRSELDRLHNMLSNQVEGGE
                     QIANLTRDMAALRENLADANNEVGRLHDELTKVSGDLAAVIKQRDQLAEERKGSQAKI
                     QSAVEQAVNQAVTELRAKAEKEKQEIIDKHNAKAQEVAELKNRLASATPSESVTQELS
                     STKSERDQYRARVAELEAMCKELQKAAAHPAPAASQGNSDAERVLAEMKGVATTAYDG
                     INDALSELRLAIVMAQEMWNKMERSYADRDAGRKLRDAIDDTMNRADEAKGHVRSLRS
                     LIE"
BASE COUNT         2244 a         3894 c         3652 g         2735 t
ORIGIN      
        1 cgtctcactg cgaggctgac cttggctgta gacgcgacgg atgttggggt ggcgctgccg
       61 caaaaatttg cttagctcag cgtcggcaac ccacacaagt ccctgctgct gctgtgtccg
      121 cttttgctcg cgcagacggt tggcaaaacc gatgatcacg cccgacagaa acgtccgtcg
      181 ctcgcggttg ctcgaaattc ccagcttggc tttgtgcgct tgccacagcg actccgccga
      241 gtgcagcaaa aaatcataca catacgccga catttcgaga ttttctggcc gccccagcac
      301 ctcgaggaca ctgccacttt tccccgtccg cacatcgaag ctcgacaccc agatggtctc
      361 gacgaaaaaa tactcgccca gcaccacggc aatcagccgc agatgttcgt cgatgcgacc
      421 gcgcggctca cccaggtggc gaaatgcttg ccggcgcaca tctcccgtcg ggatttctag
      481 gttgtacttg agcaaaagcc gctgcgcttc ctgcatcgcg ctctcggctt cgtgaacatt
      541 ttggctttcg gccagcgaca aaagtcgcgc aatccgcgta agctgccgtg ccctctcccc
      601 gtcgagcgct gtctcgttgc ttgtcggcag tcccgccgcg ctgtggtcga ttcccatgcg
      661 cgcacagact gcctgaaagg ctgatccatg tgctgtctcg tcggtgcgcc cgaggatttc
      721 atgcacatat tggtgtgcca tttcgtgctt tagcacttcg agcacaatcc cccacggttt
      781 ttcgcgcacc aacgcttccg acaaaagcaa agagcgcgtc gctggcaccc acagcccaag
      841 ccgcgaaagc gatgaagtaa gtccaatttg cgggggcata agcgcccgac gaaacagcgt
      901 cgcgttgaag tggtgccatg cccgcaaaag ctcacgcacc gtggcggcct gaagctgtgc
      961 ggacagcgaa tggggaagtt ccgcgctcat gttcctacac ttttttgcat acccgcgccc
     1021 gcttgtcacg ttgcgccgca gcgtgtcatt ttccgtcgct gttcaccgcg ctgtcagacc
     1081 gtttcgctag cgtcctcgcc atgaacccga tcgccttcgt tccttcctcc cgcgctctgt
     1141 ttgtttcgga tgcagccgac cagaccggcc tggctccggt aacgttcgcg ctgttcgata
     1201 gacttcgcct gcaaggctat ccgacgcagg ttcgactgct tctgcccggc agtgagctgg
     1261 cgttccgggt ggcgagcggt gtgatggtgg atttgctcga cgcgatgccg attctgctgt
     1321 cgcccgatgt gcagcatgaa gttctgcagt cgcgcgagct ctgcggcatc agcaaagtgc
     1381 tacggctcgc gctttctgtc gtcgagcgcg gccaggcgtg gcccaagctg tcgaggatgg
     1441 ggcacggcac ccggtttagc gcccgctgga aagccgccct gtcaccgagc gagcgcgccg
     1501 aactttgccg catcggcaaa gagctgtcgt tgtgcatcgg tgagcttttg tcgacgccgc
     1561 tgtcgagcga gcgctggcag cacatcggtg ccggtccggg tctttcgtcg ctgttgctgc
     1621 atgcaaccgt cgatttggtg gtgcgtgaag gctgccgtcg cggagcccaa gttcgactcg
     1681 ccgaatgttc gaccaagacg tgggaacagc gactggtcat ggcgctcggg gatgaaaaag
     1741 gactgttgct ttcaccactg cccagcgacg aaacgtttgc agcacggact gccgaactca
     1801 acgactgggc aggacttgcg ctttcggggt cgagccgacc gctgttgccg tcgccgccgt
     1861 cgtggcaagc gagcgaaagc ctgcaacaac tggtcagtcg atttctgcgc caagccggac
     1921 agctatcggc ggtgcttatc gccggcaagc ctgcgccgcg gatgcttccg atgagtgcgt
     1981 ggcgaaatga aaagccagaa actgagcgca aacccgccgt cctgccgcgc aagctgctcg
     2041 acgaagcgct acagcttctg cgcgacaagc gaccgggctt tgccgaccgc catcaagcgg
     2101 cgtgacccaa gtgggggatt agttcgtcga cgggctgctt tcggcagcgg gaggagcggg
     2161 cggcggcggc acgacggtgg tgggcacagg tccagtcaga accagcggca ggacttcgtc
     2221 catcttcgtc gcgtagaaaa tctccatgtc gttcttcact tcgtcgggaa catccaagat
     2281 gtccttgcgg ttgcgctccg gcaatacggc acgacggatt ccggcgcggt gcgcagcaag
     2341 caatttttcc ttgattccac cgacgggaag gacgttgcca cggagggtga tttcgcccgt
     2401 cattgcgaca tcgttgcgga ccggaattcc cgtgagcatc gacgtcagcg caacaaacat
     2461 cgtgatgcct gcactcggtc cgtctttggg catggccccc gcgggaacgt gaatgtgcag
     2521 atccgctttg tcgaggaagt gttcgtcgat gccaagctgc ttggcccggc tgcgcacata
     2581 cgacgtcgcc gtctgcgccg attccttcat cacatcgcca agctgcccgg tcagcgtcag
     2641 actgctcttg cccggcatcc gtgtcgcttc aataaacagg atgtctccac cgacgggagt
     2701 ccacgcaagt ccggtggcca cgccgggaat gctggtgcgt tccgcgactt cggacagata
     2761 tttctgcggc cccagataat cgggaatctt gtcggcgacg atggtggtct tttctttcgc
     2821 ttcgccttcg gccactttca ccgcgacgcc acgacagaca ctgccaagct ctcgctccag
     2881 ggtccgaaca ccggcttcgc gcgtgtagct gtcgatgact tcgctgacgg cggcgtcctc
     2941 gaatgcgatg ttgtcgccgt ttagaccgtg ctcgtcgacc tgcttcggaa tcaggaagtt
     3001 cttggctatc tggagttttt cactgcgggt atagcccggt atctccagca cctcgagacg
     3061 atcgcgcagc gccgccggaa tcggatccag cacgttcgcc gtcgcgatga acatcacctt
     3121 tgacagatca aacggcactt ccaggtagtg atcgctgaac gtgttgttct gttccggatc
     3181 gagcacttcc aaaagcgcgc tcgacggatc gccacggaag tcatgtccca gcttgtccag
     3241 ctcgtcgagc acgaagaccg ggttgttggt gcccgcgcgc ttgagcccct ggatgatgcg
     3301 gcccggcagc gatcccacgt aggtccggcg gtgaccgcga atctctgctt cgtcgcgcac
     3361 accaccgagg ctgacccgga caaacttgcg gccaatcgct ttggcgatgc tcttgccgag
     3421 cgacgttttg ccaacgcccg gcggaccgac caggcacaaa atcggccctt ttttgtcgtt
     3481 cttgagcttg agcaccgcca ggtattcgag gatgcgcttt ttgacctttt cgaggtcgta
     3541 atgatccgag ttgaggatgt tgcgcgcttc ctgaatgtcg attttgtctt ccgtcgacac
     3601 cgaccacggc atatcgacga gccaatcaag ataggtccgc gtcaccgtgt actcagccga
     3661 cgacggctgc atctgcttga gccggtcaaa ctgcttgagc gccacttttt cgacgtcagg
     3721 cggcatcttg gcatcgatga tcttggtctt aaactcctcg gcatcaccgc cgccttcgtc
     3781 gagttcacca agctcttcct tgatcgcctt gagctgctga cggagcacgt actcgcgctg
     3841 gttgcgaccc atttcctctt ggacttgggt gtttattttt tcgcgaacct tgagcacctc
     3901 aagctgccgc gtcagaaact gcagcacctt gcgcatccgc tgtttcagat cgaacgtctc
     3961 cagcagatcc tgtttttcct cgacgggaat gtcgagctgc gaggtaatca gatccgcaag
     4021 ctggccggct tccgtcacac tctcgacgag cgacgacgcc tctttgggca gctccggcat
     4081 gagcttgatc acccgcttgg cgatgtcttt taggttcatc accagcgcgt cgagttcgac
     4141 atccgccacc gtcggatcgg gaatcgacgt gacgcggcag ttcaggaacg ggtccgaccc
     4201 ctcgtagccc tggacgcgga agcggaccac gccctgcaaa atcaccgaga agttgtcctt
     4261 ggccagcttg atgaccttca aaatgcgcgc ggcagtcccg acggcataca tgtcaccttc
     4321 gccgggatct tcggtccgtg catcgcgctg cgtcaggatg ccgatgaccg gcttttcctt
     4381 ggcgatggct tcttcgatga gcttgacgct cttccgcctg cccacgtcga ttggaatgat
     4441 cgaaccgggg aagagcaccg agttgcgcag cggcaaaatc gagatcacat cggggatctc
     4501 gactgggccg ctctgatctg gggttccccc cttgggcttc tgcatccaac gctccttcac
     4561 tctttacgtt ggcttccgct gccgcgtggc gcattcccct gcccactcgt cggcaccggc
     4621 ttaccaaacc tcatcacaat gcggcctatg atagtgcctg gtttttgcca cgcaataccc
     4681 gaggactttg cttctgcttg ctgtttttgt cgggcctttg cacgggcaag acgtggccgc
     4741 gactgtccat ggtagatgtc accagcatgc ggcttgtgca ctacccctgt aaaaatttgc
     4801 ccattttggc cggcgagtgc gtggttcccg ccgaaataga gcgcatcgac cgtcgcaccg
     4861 cgagcgcgtg ggccaaaggg atttccgtcg acgattacct ccggcgcgag cgttttttga
     4921 agacgcggcc ttatgcagcg gggatgacca tccatgcgct gctcgacgat gacggaagcg
     4981 atttgcaggc acagattttg gcaagctgcg aaacgtatcg cgtcccggtg gtcgtcccag
     5041 gccgtcgcac aatcggtttt gggctcgggg tcgcgagtgt gtttgtcgat gaaccgctcc
     5101 gcggacatgg gtttgccgcg acgctcatcc aacacgttca cgaccacttc cgtgcccaag
     5161 gcgccgcgat gatgtacctg atgagcgaga tcggcccgac gctgtatgcc aggctcggct
     5221 atgtcacaac gccgctttcg ctgcgacgtt ttccggcggc accactgccg gctgggctgc
     5281 gggtagaacc gatccacgcc gatgagctgg cgacgctgat tccgcgcctg ccgccgccgc
     5341 gcagtccgct ttacattccc gcaaaccgcg aacagctcga ctggcatttt gcccgcggtc
     5401 agttttacgc cgggcttgcc caaggcgagc taccgacaga aattggagca cgatccgccg
     5461 acgcggtggc gatttgggat gttgatcatc gtcccgattg ccgtcgagtg cgggtgctgc
     5521 tcgttgctgc ggaaaagccc tctgtccacg tcgagcacgt tctttgcgcc gctgctgccg
     5581 cggcccacgc ggtgggtttg ccgtttgccg aagtctggga aaccgcagcc ctttcgcctt
     5641 ttctcgacgg tgggatcaaa atcgaagccg acgatttgcc gatgctgtgt ccgcttgcgc
     5701 ccgatgtctc tgcgactgat ttcctcgacg tccagcgctt tcattggctg taaacggtag
     5761 gtccgaccgc tgaacttgcc ggtccggtgc tttttcgcca tgctcccgcg catgcggatt
     5821 cccactcgtc ccacggtcat catccggcac tgtcccgact acgacgttca gcgaatcaag
     5881 cagctctttg acgaagggct aaaaacgctc gggctacggc ctacgggtcg caccctgatc
     5941 aagccgaaca tcgtcgctgc gggcccactg tttgcgaatg cgcacacccg ccccgaagtc
     6001 gccgagggac tgattttggc gctgcaagcg atggccgatg acagcgtgac cgaaatcgcc
     6061 gtcggagaac gctgcggaat caccatccct tcgcgcgcgg cttttcagga atccggtttc
     6121 gacgacatgc tcgaaaggac cggcgccaaa cgctatctgt ttgatgaagt gccgcaggtc
     6181 gaagtccgtt acagccacga aggtcgtctg cgcgactacg tctttacccc cgagccgatc
     6241 gcaaaagcgg acttctttat caacttaccg aagttcaaag cgcatccgtg gacgacggtg
     6301 acgttctgtt tgaaaaatta catcggcatt caggatgacc ggcaccgact gattgaccat
     6361 gatcaccgct taaacgaaaa gattgccgac ctgcagttca tcgtgcagcc gcagttctgc
     6421 gccatcgacg ccatcattgg cggagaaggt cgaatgctca cgccgaagcc ctttccgctc
     6481 gggcttttga tcatggcaaa cagccagctt gcgctcgacg cggtgtgctg ccatatcgtt
     6541 tcgctcaatc cgcgcagcgt ggcgcatttg cagcttgccg aagatcgcgg ctttggcacc
     6601 accgatttgt cgcaaattga tgtcattggc gacgtgacgc tcgatgaagc gaagcgccgc
     6661 gcgagtggtt tccaagtcgg actgattcgg atcgaaaaat acttcgaggg cagccgcatc
     6721 accgcctacg ccgggccacc accggaacca gagcacagcg actactgctg gggcggctgt
     6781 ccgggggcgc tgcaagaagc gattgaggtg ttgcggctct acgatgagca gtgcgaccac
     6841 aaactgccgc ggctgcatgt cgtgtttggc gcctaccgcg gaccgattcc cgcccagccg
     6901 ggcgaaaaag tcatcttcat cggggactgc gccgactggg aaggtgaatt gcaaggccag
     6961 cttgtgcaga ttcgcagcgt ctacgaagac ggacgcaagc gcgatcccta ccaggcgagc
     7021 cacgacgacg tgttcgccaa aatggcagcg gtgtcgatga agatgctgtg gaacgaaggc
     7081 acgtcgcaca tcaggctgcg cggctgtccg gtcagcgtct gtgaacaagt gctcgccgtc
     7141 gtagcgctat cggatgtcaa caatccctat ctggacatgg aacacgcggt gccgttccag
     7201 aaaaactacc tggcgtggcg agccagccaa gcgatgcagc gactgcgcgg gattccgtat
     7261 caaaaacccg gtgcctgctc gcgcggacag gcggcgcctg aagtaagtcc aaaacactcc
     7321 gagggtggcc atggatagtc cggtgcagat ggaaccgccg aaccgctggc aaaccctgcg
     7381 cgatgagctg gatttttggc gcttcttgat cccgcgggaa acggtgcagc tcgccggaag
     7441 gcatctgccg ctggaaaagc tgcgcgacct tttgcccaaa gcgctgcgcg agcgcctgcg
     7501 ttttggactg tatccagcgc gggtcagtga tccgctggcg gtcatctgtg cgcgcgagca
     7561 tccaccgtgg gggattccgg cgcagctcga tccgcaaaaa ccgctgcact ttgtcgatcc
     7621 gccgcgggtg tcgatcctga tggtcaccta tggcaactgg gatctgacgg cgctgtgtct
     7681 ggcgagtctg caacggctgg cgggatcgat cccgtttgag ctaatcgtcg tcgacaacag
     7741 cagtcccgat gacacgccgc agcggctcaa agagctggaa caatcggcgc ttctgcccat
     7801 gcgcgtcgtc ctcaatcgac acaacttggg ctttgcggcg gccaacaacc aagccgcccg
     7861 gctcgcacgc ggcgatgtgc tggttttgct aaacaacgac accatcgtga cggaagactg
     7921 gctgccgcga cttctcgcgg tgctcgacga agatccgcag gtggcgatgg tggggccgcg
     7981 gaccaactcg tgcggcaatg aagcggcgct cgggacgccg tatgccaccg tcgcggacat
     8041 gttcgcgttt gcaaaacgct acaccgatgc ccatcgcggg ggccgcaaag aacccgacat
     8101 gctgacgctg ttttgtacgg cgattcggac cagcgtgtgg caagaggtcg gcgggctcga
     8161 cgaaggctat gggctgggca tgttcgaaga cgacgacttg acgatggcgg tccggcgatg
     8221 cggccacaaa gtgctgctgt gcgaggacgc ctatgtccat cacttcggcg gcgcggcgtt
     8281 tggcaagctg ccaccgcgca agtacctgcg gctgtttttt cacaaccgcc gctacttcga
     8341 gcggaagtgg cacaccgaat ggaaaaagcg ctagccggcc tggataaggc cggtcacttg
     8401 gcgtcgtcgg gctgccactt cgacagccga cccagctcgg tctcgacgtc gagctgattg
     8461 attcccgtca gcaccgcttc gtgcatcagc tcgcgctgct cttggtgctg ggcggtaaac
     8521 aggatttcgt agacatcggc ggaaagcgaa ccaaagcgcg acttgaacgc tttggcgatc
     8581 gtcaccgcat gcccagccag cgacggcccc gcgaggtctt cgatcacttg ccggaacttc
     8641 ggctcgctca gcacctcgtg gcgaacgaac atctgatggc cgttttcgac tttgcgaacc
     8701 gtcagaccgt cggaaaagct gcgccggatg acaccatcgc tgcgctttac cgcaatggcc
     8761 cagctctggc cgtcttcgct cggctggtcg gcgggcacaa acgtgatcga gtggctgagc
     8821 ggcgccgtca ccgcaattcg caccggaaac accgaccgct cgggcccgcg tacctcgaat
     8881 cgctcgacga tgggccgtcc agcccagccg cgcttgcgca ccgtattttc aagccgcagc
     8941 gagagcacga cgcttagctg ctcaatcgct tcatagatat cccccatccg attgccccca
     9001 aagcgctcgg cgatgcccgc gctggcccac agcttggctt accctcggtt gtaaattccc
     9061 gtcgagccgg tgttgccctg ctgatagtaa tcagaggtga gctgacggtc tttgacttcg
     9121 tttccgagtg cgtcttcgag aaacttggtt tcttcgacgc acgactgacc gggaatgcac
     9181 ttcacctgga cggacacttc tcccgacgga gaaatcgtga tttcaagctc tttgcgctgc
     9241 gccatgattt gctccttgct ggcccttttg caaagccact agccccactt cgacacagag
     9301 agcttgatcg taccgtcctc ggcgacgttc tgctcctcga ttttgtaacc ctgcgcttcg
     9361 caagccacca cgaccgtttg gtaggcgtac tcgcggttta tctcgtcgac gacttcctgt
     9421 tccgttttgc cgacggtggt ttccacgccc caccagtcgg cttgcagacc gtaggtgccg
     9481 tcttcgtttt tcacgacgcc gatgtcgtat cgacccatgt tgatagaaac ttcggcggtg
     9541 cttttctggc cgcgccagcc gcgcacttcg acgccctgct ccgagtgggt gtacttgagc
     9601 ttgagcttgt cgagcgcttt tagcaggcaa gcaatgttgt tgatcttggt tgcgaccgtc
     9661 gtgaagtgac tcatcggctc tgccttccgt tacagctcaa tccgtccgcc cggctcgctc
     9721 ggcacggcaa aacgatcttt ggtcttgcgc tgcggctgct gcgacgcggg cagcgatgcg
     9781 gtccgcgcac gggttctgga ccactcacgc atgtcggcga tcttttcggc catcgtcacc
     9841 gccagcggca caatctcgcg cgccgagtgc aagagcagat cactcgaaag ctcgcggttg
     9901 tgcggatcgg cgtcgaatgc ctcgtagagc gccgagacca ccacttgctc gacctcgctg
     9961 ccggaaaatt cggccatcgc gtcgatgagc ttcggcagat cgaggtgctg gatgctgcga
    10021 ccttttttct tcaggtgaat cttgatgatc tcgccgcgct cttcctgcgt cggcagatcg
    10081 acaaagaaga tttcgtcgaa acggcctttg cgcaaaagct cgggcggcag gctgcgcacg
    10141 tcgttcgccg tcgcaatgac gaacaccggc gcggtctttt cctgcatcca ggtcacaaac
    10201 gtgccgaaca cgcgcgaagt cgtgccgccg tcggacatgt tcgacgaacc agttcccgaa
    10261 agaccttttt cgatttcgtc gagccacaaa atcgccggcg cgacagcttc cgcggtcttg
    10321 agcgccttgc gcatgttttc ttccgacgca ccgacgagac cgccgaaaat cttaccaaca
    10381 tcgagtcgca atagcggcat ctgccacgca gcaccgacgg ccttggcagt cagcgactta
    10441 ccacagcccg gcacgcccag cagcagaatg cctttcggaa gcggcagacc gaagtcgcgg
    10501 gcttccgacg aaaacgcatg ccgacgcttg accagccact gcttgagtac ttccagacca
    10561 ccgacgccgt cgagcttttc ttccgcttcg aagtattcga ggatgccgct cttgcggatg
    10621 atgtgttttt tctcggcgag aatcgtcggc aaatcgaacg tcttgtgacg gacaatcgac
    10681 ttggcgatga cgttttcggc ttcggtcaaa gtcagaccaa gcgccgcgtc gacgatctgc
    10741 tcgcgacctt cggcttcttt ggcaatgccc ggctcacagc cttgcggaag ctgatgcagc
    10801 aggctttcga cgactttgtt gagctcggga cggtttggca gatcccagtc gacgatgcag
    10861 aggtcttttt ccagctcagg cggaatcttg gtcaccgccg acaaaatgaa gacgttcttc
    10921 tgggttttgc gcagatcatg tgccagatcg cgcagcttgc gaaccaccgt cgggtcgttc
    10981 acaaatgcgt ggaagtcgcg cagcacaaac agaccgcgac cttccgccgc ggcaatgtaa
    11041 tcgagcgcct tgatcggatc tttggcgtcg ccacctttga gggtgccaaa cttttgcaca
    11101 aagccgcggg tgatcgacca cgatcccagc ttcatgtcgc ggcgtcgcgc cagcttgagc
    11161 agcgagtcct cgacgcgctg ttcttctgac gagatgatgt acataagcga atagcgcgcg
    11221 cggataagat caaggatctc cttctcgctg cggtttagct cctcgctgtc ggtcgggggt
    11281 gcagccgctt gctcttgcgt catgggttca cctgtgcggg atttggcgtc gctgtgccta
    11341 ctcaatcagg ctacggagcg agcggacatg gcctttggct tcatcggccc gattcatggt
    11401 gtcgtcgatg gcatcgcgga gcttgcgacc cgcgtcacga tcggcatacg agcgttccat
    11461 cttgttccac atctcttgcg ccatcacgat ggcgagacgc agttccgaca gtgcgtcatt
    11521 gattccgtcg taagcggtcg tcgcgacgcc cttcatctcg gccagcacgc gctcggcatc
    11581 gctgtttcct tgcgacgccg ccggggccgg atgtgccgcc gctttttgca gctctttgca
    11641 catcgcttcc agctcggcga cgcgggcgcg gtactgatcg cgttcgctct tggtcgacga
    11701 cagctcttgc gtcaccgatt cggacggcgt cgcactggcc agccggtttt tcagctcggc
    11761 gacttcttgc gccttggcgt tgtgcttgtc gatgatttct tgtttttctt tttcggcttt
    11821 ggcgcgcagt tccgtgacag cctgattgac ggcttgctcg acggcagact gaatcttggc
    11881 ctggctgcct ttgcgttcct cggcaagctg atcgcgctgc ttgatcaccg ctgccagatc
    11941 gccggatacc ttggtcagct cgtcgtggag ccgcccgact tcgttgttgg cgtcggccag
    12001 gttttcacgc agcgcggcca tgtcgcgggt caggttggca atctgctcgc cgccctcgac
    12061 ttggtttgac agcatgttgt gcaggcgatc cagctccgag cgaagctcct cgacctgcac
    12121 ctgggcacga ttttgccgct cgcgcagatt gtcacgctcg ccgcgaatcg acgacagctc
    12181 gcgctcttgc tcctcactgc gcttgcgcag gtctttgagc tgcgattcct gattgaccag
    12241 accgacgcgc tgctcctcga taagccgctc gcgttctttc aggatgtcac gcagcttgtc
    12301 gacgtcgccg atctgcgaat tgatcgcgcg catctgctca tcacgagagc gcgccatctc
    12361 ttgcagcttc tgcgtcaggt tgtcggcggc ctgcttgctg cgacggacat cttctttcgc
    12421 tgccgccagc tcgcgatctt tggcttccga ctgcgttttg agctcgtcgg cctgcgcctt
    12481 ggtcttgtca aactgctctt tgagctgctt gagatcctga cgcag
//
LOCUS       DAORGN010000148        12470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2108_length_12470_cov_5.481434, whole genome shotgun sequence.
ACCESSION   DAORGN010000148 DAORGN010000000
VERSION     DAORGN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_12470_cov_5.481434"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKL17_15150"
     CDS             complement(<1..370)
                     /locus_tag="PKL17_15150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK46121.1"
                     /translation="MLEPLEMSDVTRRSGPVVLVPAYNAGSTVGDVVAGILAKGLPVL
                     VVDDGSSDDTGDKAQAAGATVLRHPQNRGKGAAIQTGFAYAQKLGASALLTMDADGQH
                     DTAELENLLAAHKKQPDALII"
     gene            complement(372..863)
                     /locus_tag="PKL17_15155"
     CDS             complement(372..863)
                     /locus_tag="PKL17_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46122.1"
                     /translation="MRTVPSLPQTPPFRLIDRIVEYDAVVGSLVAEKQLTEGDALWPA
                     GLALSASVDRSYPQTLLVEALCQAAAAFNMLSAGESSESASAPAAEHRGYLVGVSDLR
                     FPNFVRIGETLRLSVKKQQTMGSLVSFLAEATAIALVQADTSALAPRQVVHGRLLFSV
                     MAA"
     gene            914..2137
                     /locus_tag="PKL17_15160"
     CDS             914..2137
                     /locus_tag="PKL17_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46123.1"
                     /translation="MKRQQPRRRNARKLIIWACLSIGIFASGCSGRNQRGDNLPTQGT
                     QQVQSIEMEDLHITVGKNAKNGDEDFEAYDAPTLFREADALFEADKFADAAQKYEKLV
                     VEFSDSPFAPPALFNAALSLEALGRYGEASDRYKQVVALFPSSKNAKDAALRQAACLA
                     EVGKWVQSAEALALVEKRDDLTLSDRLETMARHGLALFELKDLSGAEAKFAQSLALYD
                     AHKAEEQPQNNFFLAMSQFYVAHIAHQRFRDLPLRLPQKQLALDLDSKAKTFLLANTK
                     YVETIKLKNPTWATAAGFHVGTLYRELYDAMIAAPVPTEVAKTDSKVFYDLLLREQLR
                     HLLTKAKDVLESNVKMAERVGVKNGWVERSTEQLGELDKLLSGLDNPAPSAPGEAPKT
                     PSRPAPKDNKTRATL"
     gene            complement(2127..2693)
                     /locus_tag="PKL17_15165"
     CDS             complement(2127..2693)
                     /locus_tag="PKL17_15165"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK46124.1"
                     /translation="MPVFSLRPRVFFGLVALLWVAVFFVCVWNDQAVQPLFRLSTPAL
                     VRAGALALPLHSPLGLVRLAFSWLVHVDSIHLLSNLLALSCVAHAWPQTDRVVGPLGL
                     GVAVSGAASILAHRGAATICCGGSGVLLALLPTAVVSAPSRLRAAICLIAGVLLFGSG
                     FFPAGDWAAHVGGLAAGILWLVRRRATG"
     gene            complement(2696..3958)
                     /locus_tag="PKL17_15170"
     CDS             complement(2696..3958)
                     /locus_tag="PKL17_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNK46125.1"
                     /translation="MWLPLLLSCICLVVEAFFSSAEIAIVSADRAHIRDLAAAGNRGA
                     RLVEDFLIAPHQLLSATLLGTQMAVVLQTVVWTLWLHAHHPRHVELWLLVGLTPAVVI
                     LGEIVPKAIVRQHANRLAPLQARLLAVLMRVLAPGVYLLERISKLAASRLGIDVHHKL
                     VTREEIESLVSAQAEPTGDSAVSLSDVTEGERSMIARIFDFPSLRADDVMKPLSDVVA
                     QAETASVADLAREIGDKKYSRIPIYRERLDQIVGVVHAFDVLKSGRSDGKAKDLMREP
                     IYAPEACPALDLLVRLERAHQGMAIIVDEYGGAVGLVTVEDLLEQVVGDIEDEYDEEP
                     PALSKQRDGSYRVQARISLEQLNRELKLELPEAEDHEYESLGGLLLDRLKRIPRVGEV
                     IKIHGVSFEIASANERRVDEVILRLVRK"
     gene            complement(3960..5147)
                     /locus_tag="PKL17_15175"
     CDS             complement(3960..5147)
                     /locus_tag="PKL17_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNK46126.1"
                     /translation="MAAIRSCARLRFLMIAEAEFSSRVWQDVIQLLSHLLLLLPLFLS
                     AGFFSGTETALLSLSRTRREVFRQNAATDPASARVVWLLSDPRRLITTIIIGNELTNI
                     LLSEVMTDSVAQVFRLVPRLQFGLTAASLDVLVAMTATVLTVPLLVTFGELIPKTVGI
                     KLADRWARLVSWLLKVLTWVLWVPRVAISAISDLLLLVFVGPKSRHTEAAPVLSEREI
                     RTLVDLGQQEGEIRGAERKLIHNVLDFGDLTVGNLMTTEKKVFALPVTMPLSRIVEAV
                     SKQRYSRVPVYRGRRDNIVGILLAKDLVGYSYGDLEGQPLVDLLHPPLFVPRRAKCER
                     LLREFQKRKTHMAMVVDEYGRLAGLVTMEDLLRPLFADLARTPTLQPATSGEVPPVGK
                     GEG"
     gene            5280..6941
                     /locus_tag="PKL17_15180"
     CDS             5280..6941
                     /locus_tag="PKL17_15180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK46127.1"
                     /translation="MPPQTDQPSDSHQPTRVSRRICLVRHPHDLASPTVAWVDLAALL
                     CEAGHVVTVLDVNAVSDREATHSLFGLNPNLQHICLPPIRSLATPCSQERDSYALYEW
                     LRETCFDVIVMSFGDGIGFHTLAAKTQGIAFPNTLFCLVAQTPSQKWIGNSQQFPTGH
                     AAFRIDFMQRSLAAQVDVLIAQDETTLAALADQQYKLPSAVCVAGSLSELRTESTQTA
                     WLAALTETPIPLRKSALAALAEQSAPPLVSVGLVTFNRPQLLRQAVDSLCAQDYPQLE
                     VILVDDGSSDPDAVAALDELAPIFANRGWQILRQTNRYLGAARNAAAHMARGKYLLFM
                     DDDNVAKPHEVRTLVSVAEYTEADIVTCHLDRFSSTESPLTKPMKTERWGPLGAALSV
                     GLTENVFGDANALIRKRSFDAIGGFTELRGVGHEDWEFFAKACLQGHRLDVVPESLFY
                     YRVLPGSMLSSSKQRANLLRSATPYLHGQPLWLRQILLGYLGQHLELRAPSGGQPVLT
                     DAPPLSDDDVLRLKNRIYDALRPYPRALNLFRRSAQAVFRLAQTK"
     gene            complement(6948..8198)
                     /locus_tag="PKL17_15185"
     CDS             complement(6948..8198)
                     /locus_tag="PKL17_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46128.1"
                     /translation="MPAFFSSSRRTHGIHCGLIAAFHLGFFLVVQPVAGASEPPSQPG
                     LPSLSPTVGHPSQADPATDAEFVRAVSTPGDEPSGGVRRHYMWSNERKHDIYFEDFKG
                     LGGGYLGVGGDQNYTMAAAAGSQVLWLVDIDLEVVKLHKLYSALLRATDTPQAFVALF
                     ERKGVPLVDAALAATEPRLRKQLLVLYTQYREDLLAHLRDEISQSHTWLGDAEKYNYI
                     RKMAQKGLIVPRLGDLNGPRTMMQIADAAKAAKVTIRVVYLSNAESWFSYGVGFRRNF
                     AALPLDEQSCVVRTIKSNLLPYVRGDVWHYTMQRGTHFVRKLSESGYSSIDQVMLDAV
                     EAKQKGLSHVGVVPPAQPPADPSAAAKWRFSERQRRQKLLADGLVTRPAGNRECASEF
                     DQDRKQKAEQDLKALDKRIQTTQP"
     gene            8305..9636
                     /locus_tag="PKL17_15190"
     CDS             8305..9636
                     /locus_tag="PKL17_15190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNK46129.1"
                     /translation="MSSLSKPIAWGIGHKAHKGAGVLRVGLCALLASTFSVANAQRSD
                     GGTIAAGGEKAAPPVEKPAAQPGAADKTDKAEKTAKLPPIHDEALPPRARTLVTKPTR
                     NALVDFVLEMQNRSQAIARKYVQLLLSEHYDEANHLHDEVGGRRLEWPQLTQQVEAFT
                     AEHGQPKELTQYLTTSVGAEVQTFYFTATYKDGAEIGARVTVDHYGHVVGAAVGGEVV
                     PASKKRYDRYDNYQTKTELTLPFRGTWTATNATPGAGNGHYLNPNQRFAIDFQISVDV
                     GPGQRSNHQNDGSKNSDYYAYGQDILAPADGTVVTVVDGVPENPPGVTDIYYRFGNAI
                     TLSIGNGEYAVLGHLLPGSAKVRVGDRVSRGQVIARCGNSGNSTAPHLHFQLSDSPTL
                     SLAASLPAQFVRVVRQGVPETRVQPLTGDRLANQDEPPEKDKKPAVKNGKR"
     gene            complement(9647..10576)
                     /locus_tag="PKL17_15195"
     CDS             complement(9647..10576)
                     /locus_tag="PKL17_15195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK46130.1"
                     /translation="MTCSVLRGAALLSLPFFLSTATTDAHAGKAKAPAHAKPAAPPAP
                     KPPKPPAPKPAAYELLLTFDDGPRVDTTPKVLEALDQYGIKTVFFVNGVRFMGKGAQN
                     EKAKELMRESIKRGHIVGNHTVHHYFLCGKRGPVVGEREILDNAQLIQDATGVPPPLF
                     RTPFGSHCASLSATLHKLGIRPIGWDIDPQDWKLQDPDKILAFMKDELSRVRRARSIV
                     LFHDVQPATVEMLPKLLKWIADESEARRAKGEPPFKYVDATYLLSAPPSQLGQVGARS
                     ALTAPGAPPPAAVTPAPPAAAPTASPAVPAAKP"
     gene            complement(10623..11459)
                     /locus_tag="PKL17_15200"
     CDS             complement(10623..11459)
                     /locus_tag="PKL17_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46131.1"
                     /translation="MTRKLWSWSLLTLLLSAAPAVASPPTVPPALKVKSRYMGAHPIA
                     TVVNGYCQIDVPHTHDYAPDKPNLYQRVGDDYVFAGDPVPFGYDGQKNVYYGHHPVPV
                     HSENLPGSVPPPPTFCFIKGPHYHEYPVQHEAPGYKVQNDVIFYVGPIPPEVARVRPQ
                     VEKAIEVEYRPYVAQRPKVVVTPPPEWQGTVYVEPPPTVVVAPQNPTVVVAPQNPTVV
                     VAPQNPTVVVAPPTPAVVVAPAVPVVVGVPGAVILHGHGHGHGKGHFFHPGKGKGHWK
                     RW"
     gene            11765..>12470
                     /locus_tag="PKL17_15205"
     CDS             11765..>12470
                     /locus_tag="PKL17_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNK46132.1"
                     /translation="MKILVTAKRIPDPTQKLKLKDNALDLSGANWQLNQFDEYAVEAA
                     LRLTEDGANQAVRQGQVTVASLGPADVATQLRSALAMGADKAIRIEAKDEELDASVVA
                     KALAKIVQRDQPDLVLMGKLAADNEGNDVGQRLAAILGWPQATFACSIQVTDGGKAVI
                     VGREVDAGVELKKVRLPAVVTVDLRIVSPHAVKNGRTAPTFAYAEGPRYATLKGIRAA
                     KDKPLEQLQLAQLGVAP"
BASE COUNT         2685 a         3887 c         3598 g         2300 t
ORIGIN      
        1 cgatgatcag cgcgtcaggc tgtttcttgt gcgcggcaag caggttttcc agttctgccg
       61 tgtcgtgctg cccatcggca tccatcgtca gcagcgccga tgcgcccagc ttctgcgcgt
      121 aagcaaagcc ggtctggatc gccgcgcctt tgccacggtt ttgcggatga cgaagcacgg
      181 tcgcaccagc ggcttgggcc ttgtcgccgg tatcatcact gctaccgtcg tcgaccacca
      241 gcaccggcag acctttggcc aaaatcccgg ccaccacgtc gccgaccgtc gagccagcat
      301 tgtacgcggg gaccagcacc actgggccac ttcgccgagt cacgtcgctc atttcgagag
      361 gttccagcat atcacgccgc catcaccgaa aaaagcaggc gtccatggac aacctgccga
      421 ggagccaaag ccgatgtatc cgcttgtaca agcgcgatcg cagtcgcttc ggccagaaac
      481 gacaccagag atcccatcgt ttgctgtttt ttgacggaaa gtcgcagggt ttcgccaatt
      541 cgaacaaaat ttgggaacct gaggtcagac acgcccacta aatagccacg atgctccgct
      601 gccggcgcgc tcgctgactc ggacgattcc cccgccgaga gcatgttaaa cgccgccgcc
      661 gcctgacata acgcctcgac aagcaacgtc tgcggatagc ttctgtctac cgacgcggaa
      721 agcgcaaggc cagcaggcca cagcgcatct ccctcggtca gttgcttctc tgcgacgaga
      781 gatccaacga ctgcatcata ttcgacgatg cggtcgatca gtcgaaacgg tggtgtctgc
      841 ggcaaagaag gcacggttcg cataggagct gaaagtggtc cgatttgtga gactcttgcc
      901 aggagttcat atcatgaagc gccagcagcc tcgtcgccga aacgcacgca aactgatcat
      961 ctgggcctgt ctgtcgattg ggatctttgc ttccggatgc agcggtcgca accagcgtgg
     1021 agacaatcta ccgacgcaag ggacgcagca agtgcagtcg atcgagatgg aagacctgca
     1081 catcaccgtc gggaaaaacg ccaaaaatgg cgacgaagac ttcgaagcct acgatgcgcc
     1141 gacgctgttt cgggaagcgg acgcgctgtt tgaagccgac aaatttgccg acgctgcgca
     1201 aaagtacgaa aaactggtgg tcgagttttc tgacagtcca ttcgctccac cggcgctgtt
     1261 caacgcggcg ctgagtctcg aagcgctggg tcgctatggc gaagcgtcgg atcgctacaa
     1321 acaagtggtc gcgctgtttc cgtcgagcaa aaacgccaag gacgccgcgc tccgccaggc
     1381 cgcttgtttg gccgaagtgg gcaagtgggt gcagtcggca gaagcgctgg ctctcgtcga
     1441 aaagcgtgat gatctgacgc tttccgatcg cttggaaacc atggcccgtc acggactggc
     1501 gctgtttgag ctcaaggatc tgagcggtgc cgaggccaaa ttcgcacagt cgctggccct
     1561 ttatgacgcg cacaaagccg aagagcagcc gcagaacaac ttcttcctcg cgatgtcgca
     1621 gttttacgtg gcccacattg cccatcagcg attccgcgac ctgccgctgc gtctgccaca
     1681 aaaacagctg gcgcttgatc tcgacagcaa ggccaagacg tttcttttgg cgaacaccaa
     1741 atacgtcgag acgatcaagc tgaaaaatcc gacctgggcg acggcagccg gttttcacgt
     1801 cgggacgcta tatcgggagc tctacgacgc aatgatcgcg gcaccagtgc cgacggaagt
     1861 cgccaagacc gacagcaaag tattttacga tttgctgcta cgtgagcagc ttcgccacct
     1921 gctgacgaag gccaaagatg tgctggaaag caacgtcaag atggccgagc gcgtcggcgt
     1981 aaaaaacggc tgggtcgagc gctcaacgga acagctcggc gagctcgaca agctgctgtc
     2041 ggggctcgac aacccggcgc cgagcgcacc gggagaagcg cccaaaacgc caagccgtcc
     2101 agcaccgaaa gacaacaaga cccgcgctac cctgtagccc gacgacgcac aagccacaaa
     2161 atccccgccg ccagtccgcc aacgtgagcc gcccagtcgc ccgccggaaa aaagcctgat
     2221 ccaaacaaaa gcacgcccgc gatgaggcaa atcgccgccc gcaaacgact cggtgcagac
     2281 acaactgccg tcggtaaaag cgccagcaag acccctgatc cgccacaaca gatcgtcgcc
     2341 gcgccacgat gagccaggat cgaagccgcg cccgatacag ccacgcccag cccgagcggc
     2401 ccgacgacac gatcagtttg aggccaggcg tgcgcaacac agcttagcgc cagcaggttg
     2461 ctcagcaaat ggatgctgtc gacgtgaacc agccaagaaa aagccagtcg aacaagccca
     2521 agcggcgaat ggagtggcag cgcaagcgcc cccgcgcgga ccagcgctgg cgtcgacaaa
     2581 cgaaacagcg gctgcaccgc ctgatcgttc caaacgcaga cgaaaaacac ggcgacccac
     2641 aaaagcgcga ccagcccaaa gaaaacccga ggacgcagcg aaaacaccgg cacggctact
     2701 tgcggacaag acgaaggatg acttcgtcga cgcggcgctc attggcggac gcgatttcga
     2761 agctgacccc gtggattttg atgacctcac cgacgcgagg aatccgcttc agccgatcga
     2821 gcaaaagtcc gccgagcgat tcgtattcgt gatcttcagc ctcgggtagc tcaagcttta
     2881 gctcgcgatt gagctgttcg agcgaaatcc gtgcctgcac acgatacgaa ccgtcgcgct
     2941 gtttgctaag agccggcggt tcctcgtcgt actcgtcttc aatgtcaccg acgacttgtt
     3001 ccagcagatc ctcgacggtt acaagtccaa ccgcgccacc atattcatcg acgatgatcg
     3061 ccatgccctg gtgcgctcgc tccagacgga ccagcagatc gagcgccgga cacgcttcgg
     3121 gcgcatagat cggttcgcgc atcagatcct tggcttttcc gtcgctacgt ccgcttttta
     3181 gaacatcgaa agcgtggacc acaccgacga tctgatcgag ccgttcgcga taaatcggaa
     3241 ttcggctgta ctttttgtcg ccaatttcgc gggccagatc agccaccgac gctgtttctg
     3301 cctgggcaac gacatctgac agcggtttca tcacgtcatc ggcccgcagc gacggaaaat
     3361 caaaaatgcg agcgatcatc gaacgctcgc cttccgtcac atccgatagc gacaccgcgc
     3421 tgtcccccgt cggttcggct tgggcactga ccaggctttc gatctcttcg cgcgtcacca
     3481 gcttgtgatg gacatcgatc cccagtcgcg acgccgccag cttcgaaatt cgctccagca
     3541 gatagacgcc cggcgccaag acccgcatca gcaccgccaa gagccgggcc tgcagcggcg
     3601 caagtcgatt ggcatgctga cggacgatcg cttttggcac gatctcgccc aaaatgacca
     3661 ctgctggagt cagtccaaca agaagccaca gctcgacgtg gcggggatga tgcgcatgca
     3721 gccacagcgt ccagacgacc gtctgcaaca ccaccgccat ctgcgtccca agcagcgtcg
     3781 ccgacagcag ctggtggggc gcaatcaaga aatcttccac caggcgggcg ccacgatttc
     3841 ccgctgctgc cagatcgcga atgtgcgcgc gatcggcact gacgatggca atttcggcgc
     3901 tggaaaagaa cgcttcgacg acaagacaga tgcagcttaa aaggagcggc agccacatgt
     3961 cagccctccc cctttccgac tggcggaact tcgccgctcg tcgctggctg tagcgtcggg
     4021 gtccgtgcca gatcggcaaa cagcggcctt agcaaatctt ccatcgtcac aagccccgcg
     4081 agccgcccgt actcgtcgac caccatcgcc atgtgcgtct tgcgtttctg aaactcgcgc
     4141 agcagccgtt cgcattttgc gcgacgtgga acaaacagcg gcggatggag gagatcgacg
     4201 agaggctgtc cttccaggtc gccgtagcta tacccgacaa ggtctttggc gagcaaaatc
     4261 ccgacgatgt tgtcacgccg cccgcgatag accggcaccc gtgaatagcg ctgtttcgac
     4321 accgcctcga cgattcgcga cagcggcatc gtcaccggca gcgcaaatac tttcttttcc
     4381 gtcgtcatca aattgccgac ggtgagatcg ccaaaatcga gcacgttgtg gatgagcttg
     4441 cgctcggccc cgcggatttc gccttcttgc tgccccagat cgaccagtgt gcggatctca
     4501 cgttctgaca gaaccggcgc ggcttcggta tgacggctct ttggcccgac gaacaccagc
     4561 aacagcaaat ccgagattgc actgatcgcc acccgcggaa cccacagcac ccaggtcagt
     4621 actttgagca gccacgacac cagccgggcc cagcgatcgg ccagcttgat gccgacggtc
     4681 ttcggaatca gctcgccgaa ggtgacgagc aagggaaccg tcagcaccgt cgctgtcatc
     4741 gccacgagca cgtcgagtga cgccgccgta agaccaaact ggagccttgg caccagccga
     4801 aacacctgtg ccaccgagtc ggtcatcact tccgatagca agatgttggt cagctcgttg
     4861 ccgatgatga tcgtggtgat cagccgtcgc gggtccgata gaagccagac cacccgtgca
     4921 ctcgctggat cggtcgccgc attttgccga aagacttctc gacgggtgcg cgacaaagac
     4981 agcagcgctg tttctgtccc agaaaaaaaa cccgcagaca gaaacagcgg caacagcaac
     5041 agcaagtgag acagaagctg gatgacgtcc tgccagactc ggcttgagaa ctccgcttcc
     5101 gcaatcatga ggaaccgcag tctagcgcag ctacgaattg ctgccatgcc gttctggttc
     5161 cgcggcattc gcaattgaca ggttggtgtg ggctgtggac tcttcttttc gtcgccgcga
     5221 cccgtgtggc cgcgtgccaa cttttctttg ccaaacgctt ccacccagca aaatcacgca
     5281 tgccgccgca aacagaccag ccgtccgatt cccatcagcc cacgcgcgtc agccgccgca
     5341 tctgtctggt tcgacatccc cacgatctgg cctcgccaac cgtcgcatgg gtcgatctgg
     5401 cggcgctgct ttgcgaggct ggacacgtag ttacggtgct ggatgtgaat gctgtttccg
     5461 atcgagaagc gacgcacagt ctgtttggac tcaatccaaa tctgcagcac atctgtttgc
     5521 caccgattcg gtcgcttgcg actccttgct cgcaggaacg tgattcctat gcgctgtatg
     5581 aatggctccg cgagacatgt tttgacgtca tcgtgatgtc gtttggcgac ggaattggct
     5641 ttcatacact cgcggccaag acacagggaa tcgcattccc aaacacgctg ttttgtcttg
     5701 tcgcgcagac tccatcgcaa aaatggatcg gcaattcaca gcaatttccg acggggcatg
     5761 cagcgttccg gattgacttt atgcagcgca gtttggccgc gcaggtggat gtcctgatcg
     5821 cgcaagatga aacgacgctg gcagcacttg cggatcagca gtacaaactc ccaagcgctg
     5881 tctgcgtcgc aggatcgctg tcggagctgc ggacagaatc gacccaaacc gcgtggcttg
     5941 cagcgctcac agagacgccg attccgcttc gaaaatccgc gctcgctgca ctggcagagc
     6001 agagcgctcc accccttgtg agcgtgggac ttgtgacctt caatcgtccg cagcttttgc
     6061 gccaagcggt cgattctttg tgcgcgcagg actatccgca actggaagtg atcttggtgg
     6121 acgatggctc aagtgatccg gatgccgtcg ctgcgcttga tgaacttgca ccgatttttg
     6181 caaaccgcgg ctggcagatt ctgcggcaaa ccaatcgtta tctgggtgcg gcacggaatg
     6241 cagccgccca tatggcgcgt ggaaaatacc tgctgttcat ggatgacgac aatgtcgcca
     6301 aaccgcatga agtccgcacg ctggtttccg tcgctgaata caccgaagca gacatcgtaa
     6361 cgtgccacct ggataggttt tcatcgaccg aatcaccgct caccaagccg atgaaaaccg
     6421 agcgctgggg accgcttggt gccgcgcttt cagttgggct tacggaaaac gtctttggcg
     6481 acgccaatgc gctcatccgc aagcgcagct tcgatgccat cggtggtttt accgagctgc
     6541 gcggagtcgg acatgaagac tgggaatttt tcgccaaagc gtgtctgcaa ggacatcggc
     6601 tcgacgtcgt tcccgagtcg ctgttttact atcgggtgct gcccggtagc atgctcagca
     6661 gttccaagca gcgcgccaat ctattgcgca gtgcgacgcc gtatttgcac ggacagccgc
     6721 tgtggctgcg gcagatcctg cttgggtatc tgggacagca cctagagctg cgtgcaccgt
     6781 cgggtggaca gcctgtgctg acggatgcac cgccgctgag tgacgacgat gtgctgcggc
     6841 tgaagaaccg aatttacgac gcgctgcgtc cctatccgcg cgcgctcaac ctgtttcggc
     6901 gatcggcgca agcggtcttc aggctggcgc agacaaagta gctggggcta cggctgcgtc
     6961 gtctgaatcc gcttgtcgag cgctttgaga tcctgctcgg ctttctgttt gcgatcctga
     7021 tcaaactcac tggcgcactc acggtttccc gcgggccgcg tcacaagtcc atcggcgagc
     7081 agtttttgcc gtcgctgacg ttcggaaaaa cgccacttgg cggcagcgct tggatcggca
     7141 ggaggctggg ccggcggcac aacaccgacg tgcgacagcc ctttctgttt cgcttcgacc
     7201 gcatccagca tcacctgatc gatcgacgaa taaccgctct cgctgagctt gcggacaaaa
     7261 tgggttccgc gctgcatcgt gtaatgccac acgtcgccgc gcacatacgg caaaaggttt
     7321 gacttgatcg tccgcaccac acacgactgc tcatcgagag gcagcgctgc gaagtttcgc
     7381 cgaaaaccga cgccatagga aaaccacgac tccgcattcg acagatacac gacccggatc
     7441 gttacttttg cagccttggc agcgtcggca atctgcatca tggtgcgcgg accattgaga
     7501 tccccaagcc gcggcacgat gagccccttt tgcgccattt tgcggatgta attgtacttt
     7561 tctgcgtcgc caagccaggt gtgcgactgg gaaatctcgt cgcgcagatg ggccagcaag
     7621 tcttcgcgat actgcgtata cagcaccaga agttgtttgc gcaggcgtgg ctccgtcgca
     7681 gcgagcgcag catcgaccag cggaacgccc tttcgttcaa acagagcgac aaacgcttgc
     7741 ggggtgtccg ttgctcgcaa aagcgccgaa tacagtttgt gcagcttgac cacttcgaga
     7801 tcgatgtcga cgagccacag cacctgcgaa ccagccgctg ctgccatcgt gtagttttga
     7861 tcgccgccga cgcccagata tccaccgcca agacccttga agtcttcgaa gtagatgtcg
     7921 tgctttcgtt cgtttgacca catgtaatgg cgacgaacac cgccggatgg ctcatcgcct
     7981 ggcgtcgaaa ccgcgcgcac aaattcagcg tccgtcgctg gatcggcttg gctaggatgg
     8041 cctaccgtcg gggaaaggct cggaagcccc ggctgactcg gaggctcgct cgcccccgca
     8101 accggctgca ccacgaggaa aaaaccgagg tgaaacgctg cgatgagtcc gcaatgaatc
     8161 ccgtgggtcc gacgcgaaga agaaaaaaag gctggcacga ttgtaaatct taccacccat
     8221 ggcgatgtgg ccgcgccgaa gtttttgcag ttcctgcgca aaggccgcag tcgccggtgc
     8281 ccccgtcgtg gtagtctgca aacgatgtcc agtttgtcga aaccgatcgc gtgggggatt
     8341 gggcacaagg cccacaaggg tgcaggggtt ttgcgggtcg gcctgtgcgc gttacttgct
     8401 agcacattca gcgtcgccaa cgcccagcgg agcgacggcg gcaccattgc agctggcggc
     8461 gaaaaagccg cacctccagt cgagaagccg gctgctcaac cgggcgctgc ggacaagacc
     8521 gacaaagcgg aaaaaaccgc caagctgccg cccatccacg acgaagcact gccgccgcgc
     8581 gcacgcacgc tggtgaccaa accgacccgg aacgcgctcg tcgactttgt cctcgagatg
     8641 cagaatcgca gccaggccat cgcgcgcaag tacgtccagc ttcttttgtc cgaacactac
     8701 gacgaggcca accatctgca cgatgaagtg ggaggtcggc gcctcgagtg gccgcagctc
     8761 acccagcagg ttgaggcatt caccgccgag cacggacagc cgaaagaact gacccaatac
     8821 ctgacgacct ccgtcggtgc tgaagtgcag acgttttact ttaccgcgac gtacaaagac
     8881 ggcgccgaga ttggcgcgcg cgtcacggta gatcactacg gccatgtcgt cggggctgcc
     8941 gttggtggag aagtggttcc ggcgtccaaa aaacgctacg accgctacga caactaccag
     9001 accaaaaccg aactgaccct gccgtttcgg ggaacctgga ctgcgacgaa cgccacaccg
     9061 ggcgctggca atggccacta cctgaatccg aaccagcgct tcgcgattga tttccaaatt
     9121 tccgtcgacg tcggccctgg acagcgcagc aaccaccaaa acgacggcag caaaaacagc
     9181 gactactacg cctatggcca ggacattttg gcccctgccg atggaaccgt tgtcactgtc
     9241 gtcgatggag tgcccgaaaa tccgcccggc gttaccgaca tttattaccg ctttggcaat
     9301 gccatcacgc tctccatcgg caacggggaa tatgcagtgc tgggtcactt gctcccaggc
     9361 agcgccaaag tccgcgtcgg agatcgcgta tcacgcggac aagtcattgc gcgctgcggc
     9421 aacagcggca actcgacggc gccgcacctg cactttcagc tttccgacag cccgacgctg
     9481 agcctggccg ccagcttgcc ggcgcagttt gtccgcgtcg ttcgccaagg cgtccccgaa
     9541 acgcgcgtcc agccactgac gggtgaccga ctggcgaacc aagacgaacc accggaaaaa
     9601 gacaaaaaac cggcggtcaa aaacggcaaa cgctagcccg catgcgttac ggtttcgctg
     9661 ccggtacagc cggacttgcc gtcggagccg cggcaggtgg agcaggcgtc actgcggcag
     9721 gaggcggtgc ccccggcgcg gtcagtgcac ttcgcgcccc cacctgtcca agctgcgagg
     9781 gtggcgccga cagcagatag gttgcgtcga catacttaaa tggcggctcg cctttggccc
     9841 gtcgcgcttc gctttcatcg gcaatccact tgagcagctt cggcaacatt tcgaccgtcg
     9901 ctggctgcac atcgtggaac agaacaatcg aacgtgcccg ccgcacccgc gatagctcgt
     9961 ctttcataaa cgcgaggatt ttgtcgggat cttgcagttt ccagtcctgc ggatcgatgt
    10021 cccagccgat gggccgaatc ccgagcttgt gcagcgtggc cgacagcgac gcgcagtgcg
    10081 aaccaaacgg cgtgcgaaac agcggcggcg gcaccccggt ggcatcctgg ataagctgcg
    10141 cattgtcgag gatttcgcgc tcaccgacga ccggcccacg ctttccacac agaaaatagt
    10201 gatggacggt gtggtttccg acgatgtgac cgcgctttat cgactcacgc atcagctctt
    10261 tggctttttc gttttgcgcg cctttgccca tgaagcggac gccgttgacg aaaaacacgg
    10321 tctttatgcc gtactggtcg agcgcttcga gcactttcgg cgtggtatcc acgcgcggtc
    10381 cgtcgtcgaa cgtcagcaga agttcatacg cggctggctt tggtgctggc ggcttgggtg
    10441 gtttcggggc cggcggcgct gctggcttgg cgtgggcagg cgctttggct ttgcccgcat
    10501 gggcatcggt cgtcgcggtc gatagaaaaa aagggaggga cagcagcgct gcgccccgca
    10561 gaacagaaca cgtcacgtcg cgatggtacg ccgagcctgc ggagcgtcaa cgaacttgat
    10621 cgctaccagc gcttccagtg gcccttgccc ttgccgggat ggaagaagtg gcctttgccg
    10681 tgaccgtggc cgtgcccatg caaaatcacc gccccaggaa ccccaacaac gacgggaaca
    10741 gccggagcaa ccaccactgc cggcgtcggc ggagcaacca ccaccgtcgg attttgcggc
    10801 gcgaccacca ccgtcggatt ttgcggcgca accaccaccg tcggattctg cggcgcgacc
    10861 acgactgtcg gcggcggctc gacatagaca gtgccttgcc attcgggcgg cggcgtcacc
    10921 acgactttcg gacgctgcgc aacgtaaggc cggtactcga cctcgatggc tttttccacc
    10981 tgcggacgga cgcgcgcgac ttccggagga atcggtccga cgtaaaaaat cacgtcgttt
    11041 tggactttgt aaccgggcgc ttcgtgctgc accgggtact cgtggtagtg cggacctttg
    11101 ataaaacaga acgtcggcgg cggcggcacc gagcccggca ggttttcact gtgcactggc
    11161 accggatgat gaccgtaata tacgttcttt tgtccatcgt agccgaacgg tacgggatcg
    11221 ccggcgaaca cgtagtcatc cccgacgcgc tggtacaagt tcggcttgtc cggcgcgtag
    11281 tcgtgagtgt gcggcacatc aatttggcag tagccgttta ccaccgtggc aatcggatgc
    11341 gcgcccatgt agcgcgactt gaccttgagc gcaggcggca ccgttggtgg cgaggccaca
    11401 gccggcgcag ccgacagcag cagcgtaaga agcgaccaag accacagctt gcgtgtcatc
    11461 tacatctccc tcggagtacg tggcgtaaac aaaatcccaa aacagcccgc gttcattata
    11521 caccgtcgag aacgccgctg cctagccaag aagccgcgtg cgccctaaac ccacacatct
    11581 tggctgtcat ttgggccaac acgcagcatc caatggtcag gcgccagcat gggcagccca
    11641 ggcacggaaa gtcgggcaac tgctcggttt tggcggcgca ctagatagtg ctggtatcta
    11701 gcgctatcta gtgctatcta gtggacccag ttccaactcg cttgaacatt caaaaggagt
    11761 ggccgtgaag atcctcgtca ccgccaagcg gattcccgat ccaacccaga aactgaaact
    11821 caaggacaac gcgctcgacc tgtccggcgc aaactggcag ctaaaccagt ttgatgagta
    11881 cgccgttgag gcagcgctcc gtctgaccga agatggcgca aaccaagcgg tccgtcaggg
    11941 gcaagtgacc gtggcgtcgc tcgggccggc ggatgtggcg acgcagcttc gatcagcgct
    12001 tgcgatgggc gccgacaagg cgattcgcat cgaagccaaa gacgaagaac tggatgccag
    12061 cgtcgtcgcc aaagcgcttg ccaagattgt ccagcgcgac cagcccgacc tggtgctgat
    12121 gggcaagctc gccgccgaca atgaaggcaa cgatgtcgga cagcgactgg ccgcaatcct
    12181 cggctggccg caggcgacgt tcgcttgcag cattcaagtg acggacggcg gcaaggccgt
    12241 gatcgtcggt cgcgaagtcg atgccggcgt cgagctgaag aaagtccgtc tgcccgcagt
    12301 ggtgacggtc gatctgcgca tcgtgtcgcc gcacgcggtc aaaaatggtc gcaccgcacc
    12361 gacgtttgcc tatgccgaag gtccgcgcta cgcgacgctc aagggcattc gggccgccaa
    12421 agacaagccg ctcgagcagc tccagcttgc gcagcttggt gtggcgccgg
//
LOCUS       DAORGN010000149        12417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2120_length_12417_cov_6.353179, whole genome shotgun sequence.
ACCESSION   DAORGN010000149 DAORGN010000000
VERSION     DAORGN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12417
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_12417_cov_6.353179"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PKL17_15210"
     CDS             complement(<1..1223)
                     /locus_tag="PKL17_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNK46133.1"
                     /translation="MLVSLQTGRPTQSPSFLPTTIEDVRSRGFSELDVVMVTGDAYVD
                     HPAFGPVLIARLLEARGLRVAILAQPDWRSVTDFRRFGRPRLFFGVSAGNVDSMLNRL
                     TAQKQNRSDDPYSPGGRPGQRPDRATIVYAQRCRQAYPDVPIVLGGIEASLRRLSHYD
                     YWSDAVRRPILVDAKADLLVFGMGERPILEISERLLRGEPIDQIRNVRGTAHLLSQDE
                     TQKLAAAGASNGQPVVELPSFEDVAGSDEPSRLRFAKMSRLYHEESNPLSGRPLLQRC
                     GNRAVFLNPPALPLDSNELDAVYSLPFVRRPHPGYTQPIPAYETVKHSLRLLRGCFGG
                     CSFCSVSEHEGRVVQSRSPASVVDELRALQQLDDFSGVVSDLGGPTANMYGMACSDET
                     QARSCRRLSCVAPDI"
     gene            1414..2589
                     /locus_tag="PKL17_15215"
     CDS             1414..2589
                     /locus_tag="PKL17_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNK46134.1"
                     /translation="MALPDGVAVLDGALTLRYHSPLFAAKLPDLLVQLQNGRLPSELS
                     AAMSRVLGGAAQGSLLYEQSISGMAPRTCELSIKPLHLTAEPWLLLFARDVSERVWRE
                     RAQRESESLLGAILETCGVGLCLWDHRHRFVSLNRAFCEQAGFRPEELVGRPLAKVLA
                     TEEVERAQALFSELLAGHGKSAGSQSIELRFRRKDGSLFDGAVAASVLLRDEGRRFIV
                     GSVMDISERKEKQLELVERAMELQREAESKNQLNAQLSDKLALIERQHQQILDLSAPI
                     LDVFEGVLLLPLIGSLDDARARAITERLLCAVVSHRAKLVLLDLTAVDSLDRESASAL
                     RRMLTAIEMLGSRPILTGINPQVAVTLTRSDADLRDLAMVATLPDLKAGVAYCLRRR"
     gene            2655..2780
                     /locus_tag="PKL17_15220"
     CDS             2655..2780
                     /locus_tag="PKL17_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46135.1"
                     /translation="MIPFEEFVTAIEKYKRRKELEAAAAPQQGGRPRPDKPAPPR"
     gene            2841..3395
                     /locus_tag="PKL17_15225"
     CDS             2841..3395
                     /locus_tag="PKL17_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46136.1"
                     /translation="MILRRESRCLFAGASWLVPLAMWSGCLASTSPTPSASPSAPPRP
                     ELICQRQGAERIETANFRGGKEPDVVKVFHKNPDGSPPLYMSCKEVDLNGDGRKDMLV
                     YLDKFGRKLREEFDHDKDGVADQKSHYEAGVLVRDELDSDSDGKIDLIEHFQDGKRVR
                     IEKVTQAAPAKNEPAQSPAQASPR"
     gene            3446..3829
                     /gene="apaG"
                     /locus_tag="PKL17_15230"
     CDS             3446..3829
                     /gene="apaG"
                     /locus_tag="PKL17_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNK46137.1"
                     /translation="MSDTTTHGIRVQVKTRYSPDRSQPEARNYFFVYTITISNVGDTP
                     AQLVSRHWIITDATQKVEEVRGPGVVGDHPFLQPGQSYTYSSGCMLRTEFGTMEGSYQ
                     MVRPDGTRFDAEIAPFLLALPHAVN"
     gene            3829..4674
                     /gene="murI"
                     /locus_tag="PKL17_15235"
     CDS             3829..4674
                     /gene="murI"
                     /locus_tag="PKL17_15235"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNK46138.1"
                     /translation="MPHRRSRPIGVFDSGVGGLTVVSAVLSRLPHEHIVYLGDTARVP
                     YGTRSAGTVLKYSRNAARFLLRHDVKLLLIACNTASAAALPGLRAELPVGVLGAVEPG
                     AQAAAHVTRSGEVGVLGTLATIRSGAYERALLALSPSLRIHVQPCPLLVPIVEEGWND
                     ESDPVSQLVVRRYLHDLRERAPRLDTLVLGCTHYPLLRPTLQSVASELWGHPVTLVDS
                     AEAMAVATEKLLSDQDLLATGDESVDLHPTDRLRCCVTDEARVADVAARFLGRPLPQV
                     ELVDL"
     gene            complement(4690..4899)
                     /locus_tag="PKL17_15240"
     CDS             complement(4690..4899)
                     /locus_tag="PKL17_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46139.1"
                     /translation="MRHIAITDFSGEICQGEIHKEPIPRKELTARFRLPVYPSLGTDS
                     RPQIRWRCARCGKIWDREPLPQEAL"
     gene            complement(4906..5622)
                     /locus_tag="PKL17_15245"
     CDS             complement(4906..5622)
                     /locus_tag="PKL17_15245"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNK46140.1"
                     /translation="MENRIHHARLRSFRPLANQTVEYIFSHAEPELAFRPGQFLSLRV
                     GIDALENPVLRSYSIASSPEQRGELRLVLRILEDGIGSRFFSALSPGDEVTFTGPMGF
                     FVNDLSHPGDVVYVATGTGMAPILPMLHETLARPETGRVTLFWGLRTEEDLFWQDELA
                     SLGSKHPRFTPHVFLSQPKGFWRERGRVVPPVVDLLPTLQRPIFYLCGNGQMIEECKA
                     ALMARGVERKKQIRTEAFFD"
     gene            5641..6435
                     /locus_tag="PKL17_15250"
     CDS             5641..6435
                     /locus_tag="PKL17_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46141.1"
                     /translation="MLKSRRALAALAVLWIWQGGCEKRDRAVPPAPPSLPPLAAGKAP
                     AEPSKKALPPQMPSNHMPVLQGAMPVPQAGAETATVLASGTIKIADAVKDKVKMGAVL
                     FLSGRSVGQGGAPGMVLLAKKLMVTSSEIAFQLTSDDLMGMGGAPTGPVLLQGRVDQD
                     EDASTKQPGDVEGQLGPLTLPARSLTVVLETVRKEGSGPPADSTFGHGAMPPEHGSLP
                     PGHVPIPSAQGELPPGHIPVPVAKPTQAADDKALPKATGAKPAGHP"
     gene            6554..7441
                     /locus_tag="PKL17_15255"
     CDS             6554..7441
                     /locus_tag="PKL17_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46142.1"
                     /translation="MTALSSLRLGLSLLGLWLSSGCATIPRPVPVAAQPLASAQVAGV
                     SVHVPRLDPNDYPNDVLDVMTPVLVVIENRSSSEILINPDGFVLAAPGGMQYSPLRPE
                     QLALKDEPAKDKNPVEGQVLLAWRGGGGVHIGGGVSGVRSAPVYRGPSGGTVVRSPAP
                     SFRPGGGVYSHPSYYGRPGVYGRPGFYGRPGVYVRPWWGWNYPWLWGAGWGLSWYGSP
                     WWWQNQGYSTWSRADAAQMALPAGRLPAGGRTGGFLYFPKLPGGEGTSLVLAWRIHDS
                     SGQQVLGDVQVPLELTEQQ"
     gene            7445..7597
                     /locus_tag="PKL17_15260"
     CDS             7445..7597
                     /locus_tag="PKL17_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46143.1"
                     /translation="MRRFLGGALLALLAVGTGCQSSTKNPSQVWIDYYQSELNLVLTD
                     HEPPPF"
     gene            7607..8026
                     /locus_tag="PKL17_15265"
     CDS             7607..8026
                     /locus_tag="PKL17_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46144.1"
                     /translation="MRAGGSPSTLEAAFTLRDKLPAGTWGVIGDGMVLGTGANNVRVR
                     FEVRTRAASAVDDRADQVLVGVENVFHRDAANPYNAVQFEAAADGQASDAQAGDKLVL
                     RIVALPNDSDPGAMYIPNADGVYHAGRIPSLKLPPSP"
     gene            complement(8072..8479)
                     /gene="cutA"
                     /locus_tag="PKL17_15270"
     CDS             complement(8072..8479)
                     /gene="cutA"
                     /locus_tag="PKL17_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNK46145.1"
                     /translation="MSTIEHPLANGSDLVVLLCTFPSGNEWGEIAQTLLSERLCACVN
                     QVREVTSVYRWQGEIVTNREVVCLFKTRSDRYAELVARLTELHPYDVPEIVRLSVESV
                     NQAYLDWVMTECDETAASSRTDGEASTHELDEV"
     gene            8646..9341
                     /locus_tag="PKL17_15275"
     CDS             8646..9341
                     /locus_tag="PKL17_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46146.1"
                     /translation="MTTSVVPEQVAPYEVFADCPLISGTPLVAHLLGRRFEQRLNERT
                     DQVFDSDMQKALLKTLSHLCAVLGCSFGYADRTDLLLFAISQGSDARRLLARIAGESS
                     AKLSLMVNEVFTFDVRLFEVPSVELARTYFQWRRQRAESAAIERYLTHVLEGAKTAPQ
                     TIQAILGGTGSEQRIAILRQHGVEYSALPAWQRHGSAVYMSQEDGKQPQLVIDLRLPD
                     AASYPDYLARHLV"
     gene            9405..10433
                     /locus_tag="PKL17_15280"
     CDS             9405..10433
                     /locus_tag="PKL17_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNK46147.1"
                     /translation="MRSLASWRRILNVAFAVAIALASGSAQAASKHHRKPTVAHAKPN
                     RLVKKKPQRRVRQRAVPTYTRAGLPNIQAHAAVVLDLSTGNPIYQKNPDEVRPIASIS
                     KLMAMMVVLDHKLDLDGETTISQDDARRTTRGAKSRLMVGMKLANRDLLHAALMASDN
                     RAVLALGRAVGLSPAQYAQEMTSKARSLGLHKTEFHDPTGLDYGNVSSPRETVTMLLA
                     ALKNPLIAKACKTRAYVAKVLGPKKRTIDYSNTDVLLASRHEVLGGKTGYNDRAGYCL
                     VVAAKLADPAGKGREVAMAFLGEDGKMSRFADFGRAAQWLIEKRPTETAKANTGNKTA
                     KLADSSRG"
     gene            complement(10430..11380)
                     /locus_tag="PKL17_15285"
     CDS             complement(10430..11380)
                     /locus_tag="PKL17_15285"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNK46148.1"
                     /translation="MKNSSASLLSAVGFALLSLVAAPAQVNAAGATPKTRPQRQGEAY
                     TFNLRFLGSVDAGRARLAIAPPVQQGDLSVIQIVAEAEALGLAKAISGLHEHYRLVLD
                     AATLLPRSIQLEESGWRVRTAKITLDGKTVDLAITRPQGEQRAKVAMPSEPLDPLAAL
                     LLLRAMRLHDGDKLDLIVLDGSAFYQGTMEVVAHEELTTPNGPQKAIKILCRGERIAN
                     GGHKIGKPPRFGMVWVSDDAARIPLRIEGDTELGKAEFVLTSFEPGRRPLPIPKKVVG
                     VTERRQESAPKSPPTAAVETATATVQKSSETPASGTATSN"
     gene            complement(11446..>12417)
                     /locus_tag="PKL17_15290"
     CDS             complement(11446..>12417)
                     /locus_tag="PKL17_15290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46149.1"
                     /translation="FLLEREGRQDVVKIVDFGLAKLDEGQRLTQDGTVVGTAEYISPE
                     QVTGRDTDARSDQYALGCILYEMLTSILPFDGPTTTDVVYKHVYKQPIPPRKVRPEAH
                     IPEALEQVIARCMAKKPADRFEKMSDVEHALSAIEQVLLGSSNAKVVLPPPASRLPRI
                     LLAVGGIAILGTLAATIFFVLRSDGPTPPPTLVLDLSTTRPKSEAAVDLGHAPSPKDA
                     GSLADDPNAGKSGQAIDPTVKSPPVVTPPVSKPPIRVKLSMSPKKALATVTCGSETLG
                     CSPTCTVNIPGDGECEVSAPGFKPKSLAASQAKRKGTWRIVLSKAKR"
BASE COUNT         2361 a         3689 c         3933 g         2434 t
ORIGIN      
        1 cagatatccg gcgcgacaca cgacaaccgg cgacagcttc gcgcctgggt ttcgtcgctg
       61 catgccatgc cgtacatgtt ggcggtcggt ccgcccagat cgctgaccac gccgctgaaa
      121 tcgtcgagct gttgcagggc gcgcagctcg tcgacaacgc tggcgggact gcggctttgg
      181 acgacgcgac cttcgtgttc ggacacgctg caaaacgaac aaccgccgaa acagccacgc
      241 aaaagccgca gcgagtgctt gaccgtctca tatgctggaa tcggctgcgt gtaacccgga
      301 tggggcctgc gcacaaacgg cagcgaatac accgcatcga gttcgtttga atcgagtggc
      361 agcgccggcg gattcaaaaa aaccgcgcgg ttgccgcagc gctgcagcag gggccgaccc
      421 gacagcggat tcgattcctc gtgataaagc cgcgacatct tggcaaagcg cagccggctt
      481 ggttcatcac tgcccgcaac gtcttcaaag cttggcagct cgacgacagg ttgcccattc
      541 gatgcgcccg ccgccgccag cttttgcgtt tcgtcttgcg acagaagatg cgcggtgccg
      601 cggacgttgc gaatttgatc gatgggctcg ccgcgcaaaa gccgttcgct gatttccaaa
      661 atcggacgtt cgcccatgcc aaagaccagc agatcggcct ttgcatcgac gagaatcggt
      721 ctccgcaccg cgtccgacca gtagtcgtag tgcgacagcc gacgcagcga cgcttcaatt
      781 cctccgagca caatcggcac atcgggatag gcctgccgac agcgctgcgc atagacgatg
      841 gtggcgcgat cggggcgctg accaggacga ccaccagggc tgtacggatc gtcgctgcgg
      901 ttctgttttt gcgccgtcag ccggttgagc atcgaatcga cgttgcccgc cgacacgccg
      961 aaaaacagcc gcggacgacc aaaacgacga aaatcggtga cgctgcgcca gtccggctgg
     1021 gccaaaatcg ccacccgcag cccgcgtgct tcgaggagcc gcgcaatcag caccggacca
     1081 aacgccggat ggtcgacgta ggcatcgcca gtgaccatca caacatccag ttcggaaaaa
     1141 ccgcgcgacc gcacgtcttc aatcgtcgtc ggcaaaaacg acggcgattg ggtgggtcgg
     1201 ccagtttgca gcgagacgag catgaaaaac gccgcagttc taccgtgcga cgacaaaaag
     1261 ggcaattgac agctctttag acttggtgat ttcctaaaat tacccagagt ctaatcgagc
     1321 gcttacatcg tgtccgacga agctcagccg atagtagagg ggcaaaaaac cgtcccaacc
     1381 gactggccag acccttctgt ggcggcggtt ttcatggcct tgcccgatgg cgtggctgtc
     1441 ctcgatggcg cgctcacgct gcgctaccac agcccgcttt ttgccgccaa gctccccgat
     1501 ctgcttgtgc agctgcaaaa cgggcggttg ccaagcgagc tgtccgccgc gatgtcgcgg
     1561 gtgctcggtg gtgccgcgca aggctcgctg ctgtatgagc agtcgatttc gggcatggcg
     1621 ccacgcacct gcgagctgtc gataaagccg ctgcatctta ccgccgagcc gtggcttttg
     1681 ctgttcgccc gcgatgtcag cgagcgggtt tggcgcgaac gggcgcagcg cgaaagcgag
     1741 tcgctgctcg gggcgatttt ggaaacctgc ggtgttggcc tgtgcctgtg ggaccaccgc
     1801 catcgcttcg tgagcctaaa tcgcgcattt tgcgagcagg ccggctttcg tcctgaagag
     1861 ctggtcgggc ggccacttgc caaggtgctg gccaccgaag aagtggagcg agcgcaggcg
     1921 ctgttttcgg agcttttggc cgggcacggc aagtcggcgg gttcgcagtc catcgagctg
     1981 cgcttccgtc gcaaagatgg ctcgctgttc gatggcgcgg tcgccgcgag cgtgcttttg
     2041 cgcgacgaag gccggcggtt catcgtcggc tctgtcatgg acatttccga gcgcaaagaa
     2101 aagcagctgg aactggtcga gcgggcgatg gagcttcagc gcgaggccga aagcaagaat
     2161 cagctcaatg cgcagctttc cgacaagctg gcgctcatcg aacggcagca tcagcaaatt
     2221 ctcgacttgt cggcgccgat tttggatgtg ttcgaaggcg tgctgctttt gccgctgatc
     2281 ggatcgctcg acgatgcgcg ggcgcgggcg atcaccgaac ggctgctttg tgccgtggtg
     2341 tcccatcgcg ccaagctggt gctcctagat ttgaccgcgg tcgattcgct ggatcgagaa
     2401 agtgcgtcgg cgctgcggcg gatgctgacc gcgattgaga tgctaggctc gcggcccatc
     2461 ttgaccggaa tcaaccccca agtggcggtc acgctcactc gaagcgatgc cgatctgcgt
     2521 gatctggcga tggttgccac actgcccgat ctaaaagcgg gcgttgcgta ctgtctgcgc
     2581 cgtcgctagc cgcgtctcct cgggaaaccg cgagtgcttt actgccgcaa aagtcgtttc
     2641 gtaatattcc ttacatgatt ccatttgaag agttcgtcac tgccatcgaa aagtacaagc
     2701 gtcgcaagga actggaagcc gccgctgcac cgcagcaggg tggtcgtccg cgtcccgaca
     2761 agccggcgcc accccgctag cgtgcgcgtg cttttttatc cctgtttacg gcgcgcgggc
     2821 gcacagctat cgtgacgaag atgatcctgc gtcgcgaaag tcgctgtttg tttgcaggtg
     2881 cgagctggct tgtgccgctc gcgatgtggt cgggctgtct ggcgagcacg agcccgacgc
     2941 cgagcgcaag tccgtcggca ccgccgcgtc ccgagctgat ctgtcagcgt cagggcgccg
     3001 agcggatcga gacggccaac tttcgcggcg gcaaggaacc cgatgtcgtc aaggtattcc
     3061 acaagaatcc cgacggcagc ccgccgcttt acatgtcctg caaggaagtc gacctaaacg
     3121 gcgatggacg caaggacatg ctcgtctatc tcgacaagtt tggtcgcaag ctccgcgagg
     3181 agtttgatca cgacaaagac ggcgtcgccg atcagaaaag ccattacgaa gccggcgtgc
     3241 ttgtgcgcga tgaactcgac agcgattccg acgggaaaat cgatctgatc gagcactttc
     3301 aagacggcaa gcgcgtgcgc atcgaaaagg tgacgcaagc ggctccggcc aaaaacgaac
     3361 ccgcacaatc ccccgcgcaa gcaagtcccc gctaagcgca aaaatcgcta gtatctgcgg
     3421 ctcgcctagt tttcccgagg tcacgatgtc cgacacgacg acgcacggca ttcgcgtgca
     3481 ggtcaagacg cgctactctc ccgatcgctc gcagcccgag gcgcgcaatt actttttcgt
     3541 ctacaccatc acaatcagca acgtaggcga cacgccggcg cagcttgtca gtcgtcactg
     3601 gatcatcacc gacgcgacgc agaaagtcga agaagtccgc ggccccggtg tcgtcggaga
     3661 tcatcccttt ttgcagccag gccaaagcta cacctattcg tcgggctgca tgctccgcac
     3721 cgagtttggc accatggaag gcagctatca gatggtgcgc cccgacggca cgcgcttcga
     3781 tgctgagatt gctccgtttc tgctcgcgct gccccacgcg gtgaactgat gccccatcga
     3841 cgcagccgac cgatcggtgt gtttgactct ggcgtcggcg gactgacggt ggtttcggcg
     3901 gtgctgtctc ggctgccgca cgaacacatc gtctatttgg gcgacaccgc gcgggttccg
     3961 tatgggacac gcagcgccgg tacggtgctc aaatattcgc gcaatgcggc gcgttttctt
     4021 ttgcgccacg acgtcaagtt gctgctgatt gcctgcaaca cggcgtcggc ggcggcgctg
     4081 ccagggctgc gtgccgagct gccggtcggg gtactgggtg ccgttgagcc aggcgcgcaa
     4141 gcggcggccc atgtgacgcg cagcggtgaa gtcggggtgt tgggaacgct ggcgaccatt
     4201 cgatctgggg cttatgaacg ggcgcttctg gcgctttcgc cgtcgctgcg catccacgtc
     4261 cagccctgtc cgcttttggt tccgatcgtc gaagaaggct ggaatgacga aagcgatccg
     4321 gtcagccagc ttgtcgttcg tcgctatttg cacgatctgc gcgaacgggc gccgcggctt
     4381 gacaccttgg tgctcggctg tacgcactat ccgctgctgc gtccaacgct ccagtcggtg
     4441 gcgagtgagc tgtggggcca tccggtaacg ctggtcgatt cggcggaagc gatggcggtg
     4501 gcgacggaaa aactgctttc tgatcaggat ctgctagcga cgggtgatga atcggtcgat
     4561 ctgcatccca ccgatcgttt gcgctgctgt gtcaccgacg aagcgcgggt ggcagatgtg
     4621 gcggcgcgct ttctgggtcg accgttgccg caggttgaac tcgtcgattt gtagacggcc
     4681 tgggcgcggt tacagcgctt cttgcggcag cggttcacga tcccagattt tgccgcagcg
     4741 cgcacagcgc cagcgaatct gcggccttga gtcggttccc aaagatggat agaccggcaa
     4801 gcggaaacgg gctgtgagct ccttgcgcgg gatcggttcc ttgtggatct cgccttggca
     4861 aatctcgccg gaaaaatcgg tgatggcgat gtgtcgcatg ggcaatcagt caaaaaatgc
     4921 ttcggtgcgg atctgttttt tgcgctcgac gccacgggcc atcagcgccg ctttgcactc
     4981 ctcgatcatc tgcccgttgc cgcacaggta aaaaatcgga cgttgcagcg tcggtagcag
     5041 atcgacaact gggggaacga cacgaccgcg ctcgcgccaa aagcctttgg gctgcgacag
     5101 aaagacgtgt ggtgtaaagc gcggatgttt gctgccgagc gacgccagct cgtcttgcca
     5161 gaacagatcc tcttcggttc gcagacccca aaacagcgtg acacggcctg tttcgggtcg
     5221 cgcgagcgtt tcgtgcagca tcggcagaat cggcgccatg ccagtgcccg tcgcgacgta
     5281 gacgacatca ccgggatggg acagatcgtt tacgaaaaaa cccatcgggc cggtaaacgt
     5341 cacttcgtcg ccaggggaca gtgcggaaaa aaagcgcgat ccgatgccgt cttccaagat
     5401 gcggagcaca agccgcagct cgccgcgctg ttccggcgac gacgcaatcg aatagctgcg
     5461 caacaccgga ttttccaggg catctatgcc gacacgcagc gacagaaact ggccgggccg
     5521 aaaagcaagc tccggctcgg cgtgggaaaa aatgtattca acggtttggt tggccagcgg
     5581 acgaaaggaa cgcaaccgcg cgtgatggat tctgttttcc acgggtggta acgtagcgct
     5641 atgttgaaat ctcgtcgagc cttggctgcg cttgctgtgc tttggatctg gcagggcggc
     5701 tgtgaaaaac gtgatcgagc ggttcctccg gcgccgccga gcctgccgcc gttggccgcg
     5761 ggcaaagctc cagccgaacc gagcaaaaag gcgctgccgc cgcagatgcc gtcgaatcac
     5821 atgccggtcc tgcaaggtgc gatgccagtg ccgcaagctg gggctgaaac cgcgacggtg
     5881 ctggccagcg gaacgatcaa aatcgccgac gcggtaaaag acaaagtgaa aatgggcgcg
     5941 gtgctgtttc tgtcgggccg cagcgttggg cagggtgggg cgccgggcat ggtgctgctc
     6001 gcgaaaaagc tgatggtgac gagtagcgaa atcgcgtttc agcttaccag cgacgatctg
     6061 atgggaatgg gcggggcgcc gacggggccc gtgctattgc aaggccgcgt cgatcaggac
     6121 gaagacgcca gcaccaaaca gccgggcgat gtcgaaggac agcttgggcc gctgaccttg
     6181 ccggcgcgat cgctgacggt ggtgctcgaa accgtgcgca aggaaggttc gggtccgcct
     6241 gccgattcga cgttcggtca tggcgcgatg ccgccagagc atgggtcgct gccgccggga
     6301 catgtgccca ttccttctgc gcaaggagag ctgccccccg gacacattcc ggttccggtg
     6361 gccaaaccaa cccaagctgc ggacgacaaa gcgcttccga aagcaacggg cgcaaaacca
     6421 gccgggcatc cgtaaaccaa acgccgcggt gacaatttgc gatagtggcg aaaggtcgtc
     6481 gtcggcgcta gcttaagtgg aaacggagcc ggttttagtc ggcatcgaaa cgaaagcaaa
     6541 ctagaggaaa cccatgactg ccctctcttc ccttcggttg ggtctttcgc ttttgggtct
     6601 ttggctttcg tcgggctgtg cgacgattcc tcgcccggtg ccggtggccg cgcagccttt
     6661 ggcgtcggcg caggtggctg gggtttcggt ccatgtgccg aggctcgatc caaacgacta
     6721 tccaaacgac gtactcgacg tgatgacgcc ggttctggtg gtgatcgaga accgctcatc
     6781 gtcggaaatc ttgatcaatc cggacggttt tgtgctggca gcgcccggcg gcatgcagta
     6841 ttcgccgctg cgacccgaac aactggcgct caaagacgaa ccggcgaaag acaaaaatcc
     6901 cgtcgagggg caggttctgc tggcctggcg cggcggtggt ggcgttcaca taggaggcgg
     6961 cgtatcgggc gtccgttcgg caccggtcta tcgcggtccc agcggtggca cggtggtgcg
     7021 ttctccggca ccgtcgtttc gtcccggtgg tggcgtctac agccacccga gctattacgg
     7081 gcggcccggt gtgtacggac ggcctggatt ctacggacgg cctggcgtct atgttcgtcc
     7141 gtggtggggc tggaactatc cgtggctgtg gggggctggt tggggtcttt cgtggtacgg
     7201 cagcccgtgg tggtggcaaa accagggcta ttcgacgtgg agccgggccg atgcggcgca
     7261 gatggcactt cctgcgggcc gactcccggc tgggggacgg accggcggct tcctctattt
     7321 cccgaagctg cccggcggag aaggtacgtc gctggtgctg gcgtggcgga ttcacgacag
     7381 cagcgggcag caagtgctgg gcgatgtcca ggttccgctt gagctgacgg agcagcagta
     7441 ggcgatgcgt cgttttttag gcggggcgct ccttgcgctg ctggcggtcg gaacgggctg
     7501 ccagtcgtcg acgaaaaatc cttcgcaggt gtggatcgac tattaccaaa gcgaactgaa
     7561 tttggtgctt accgaccacg aaccgccgcc tttttgagcc cctggcgtga gggccggtgg
     7621 ctctccttca acgctggaag cggcgtttac gcttcgtgac aagctgcccg cggggacgtg
     7681 gggcgtgatc ggggatggga tggtcctggg gaccggcgca aacaatgtgc gtgttcgttt
     7741 tgaagttcgc acgcgcgccg cttctgccgt cgacgatcgg gccgatcagg tgctcgtcgg
     7801 ggtggaaaat gtcttccacc gcgatgccgc caatccatat aacgcggtgc agtttgaagc
     7861 cgctgccgat ggccaggcat ccgatgcgca ggcgggcgac aagctggttc tgcggatcgt
     7921 ggcgctgccc aacgacagcg atccgggcgc gatgtacatc ccaaacgccg acggtgtgta
     7981 tcacgcagga cgcattccca gtctcaaact gccgccgtcg ccgtaggtct ctttcggacg
     8041 gcgagcttgc aacaagctgg cgtcgcgccg actacacttc gtctagctcg tgcgtggacg
     8101 cttcgccatc ggttcggctg gatgcggcgg tttcatcaca ttccgtcatc acccaatcga
     8161 gatacgcctg attcactgac tcgacggaca gccgaacgat ttccggcacg tcatacgggt
     8221 gcagctcggt aagtcgagcg accagctcgg catagcgatc ggaccgcgtc ttgaacagac
     8281 agaccacctc gcggttggtg acgatctcgc cctgccagcg atacaccgaa gtgacctcgc
     8341 gcacttggtt gacacaggca cagagccgct cactgagcag cgtttgtgca atttcacccc
     8401 actcgtttcc cgacggaaac gtacagagca aaaccaccag atcggacccg ttggcgagcg
     8461 gatgttcgat tgtcgacatg ttaccagtat agaggcaggt agcttctcga cgaaaggtcg
     8521 cccgcttcga aaacttgtcg cgccgcacct gctgtcctac atggctctgc gcgcgggcaa
     8581 aaatgccagc cccaaattgc gcaaactatc tcggcaaaaa gcaaaaattg gtataatcac
     8641 gaacaatgac aacctctgtg gtcccagagc aggtggcccc gtatgaggtc tttgccgact
     8701 gtccactgat tagcggcacg ccgctggtgg cgcatctctt ggggcggaga ttcgaacagc
     8761 ggctcaatga acgaacagac caggtgtttg acagcgacat gcaaaaagcg ctgctcaaga
     8821 ccttgtcgca tctgtgcgcg gtgctgggtt gtagctttgg ctatgccgat cggaccgacc
     8881 ttcttctgtt cgcgatttcg caaggcagcg atgcgcggcg gcttttggca cggattgcgg
     8941 gtgaatcgtc ggcgaagttg tcgctgatgg tgaacgaggt gttcacgttc gacgttcgtc
     9001 tgtttgaagt accaagcgtc gagttggcgc gcacgtattt ccagtggcga cggcagcgag
     9061 ccgaatcggc agcgattgag cggtatctga ctcatgttct cgaaggggcc aagacggcgc
     9121 cgcagacgat acaggcgatc ttgggcggca ccggatcaga gcagcggatt gcgattttgc
     9181 gtcagcacgg cgtcgaatat tccgccctgc cggcgtggca gcgtcacggt tccgcggtct
     9241 acatgtcgca agaagatggc aaacagccgc agcttgtcat cgatttgcgc ttgcccgacg
     9301 cggcgagcta ccccgactac ctcgcgcgcc atttggtctg accagaccgg tttctcgacg
     9361 aaaaattttg cttccatcca ccgataaagt ggatactccc gctcatgcga tctctcgcat
     9421 cgtggcgacg aattctcaat gttgctttcg cggttgccat cgcgcttgcg agtggctcgg
     9481 cgcaggcagc aagcaaacac catcgcaaac cgactgtggc gcacgccaag ccgaaccggc
     9541 tggtcaaaaa gaaacctcag cggcgtgtcc ggcagcgcgc ggtgccgact tatacgcgcg
     9601 cgggtctgcc caacatccag gcgcacgcgg cggtcgttct tgatttgtcg acgggaaatc
     9661 cgatttatca gaagaatccc gacgaagtgc ggcccatcgc gtcgatctcc aagctgatgg
     9721 caatgatggt cgtgctggat cacaagctcg atctggatgg agagacgacg atctcgcagg
     9781 acgacgcgcg gcggacgacc cgcggggcca agtcgcggct tatggtgggg atgaagcttg
     9841 cgaaccggga tctgctgcat gcggcgctga tggcatcgga caaccgggca gtgctggcgc
     9901 tcgggcgtgc agtcgggctg tcgccggcac agtacgccca ggaaatgacc agcaaagcgc
     9961 gcagcctggg tcttcacaag acggaatttc acgatccgac agggctcgat tacggcaatg
    10021 tgtcgtcgcc gcgagagacg gtgacgatgc tgcttgcggc gctgaaaaat ccgctgattg
    10081 ccaaggcctg caaaacgcgc gcgtatgtcg ccaaggtgct ggggccgaaa aaacggacga
    10141 ttgattacag caacaccgat gtgctgctgg cgtcgcggca tgaagtgctt gggggcaaaa
    10201 ccggatacaa cgaccgcgct ggctattgtc tggtcgtcgc ggccaagctg gccgatccgg
    10261 cgggcaaggg ccgggaagtc gcgatggcgt tcctcgggga agatggcaag atgagccggt
    10321 ttgccgactt tggtcgcgct gcgcagtggc ttatcgaaaa gcgaccgacg gaaacggcga
    10381 aagcgaacac cggcaacaag accgccaagc tcgccgacag cagccgcggt tagttcgacg
    10441 tggcagtgcc cgaagcgggc gtttcgctgc ttttttgaac cgtggcggtg gctgtttcga
    10501 ccgctgcggt gggtggactc ttgggcgctg attcctgacg gcgctcggtc acgcccacaa
    10561 cttttttggg tatcggcaag gggcggcgtc caggttcgaa actggtcagc acaaattccg
    10621 ctttgccgag ttcggtgtcg ccttcgatgc gcagcggtat tcgggccgca tcgtcgctga
    10681 cccagaccat gccgaagcgc ggtggcttgc cgatcttgtg tccgccgttg gcgattcgct
    10741 cgccgcgaca caggatcttg atggctttct gcggaccgtt tggtgtcgtc agctcttcgt
    10801 gggcgacaac ttccatcgtg ccctgataga aagcggaccc gtcgagcacg atcaaatcca
    10861 gcttgtcacc gtcgtggagt cgcatcgccc gcagcaaaag cagcgcggcc agtggatcga
    10921 gcggttccga cggcatcgcc accttggctc gctgttcccc ctgcggacgg gtgatcgcca
    10981 gatcgacggt ttttccatcg agggtgattt ttgcggtgcg tacccgccag ccgctttctt
    11041 cgagctggat gctccgcggc aaaagtgtcg ccgcatccag caccaaccga tagtgttcgt
    11101 gcagtcccga aatcgccttg gccagcccca gcgcttcggc ttctgcgacg atctggatga
    11161 ccgacaagtc cccttgctgc acaggcggcg caatcgccag ccgagcccga cccgcgtcga
    11221 cagaacccag gaaacgcagg ttgaacgtat acgcttcgcc ttggcgctgt ggccgggtct
    11281 ttggcgtcgc gccggctgcg ttcacttgtg ccggagccgc gaccagcgaa agcaatgcga
    11341 agcccacagc ggaaagcaga ctggctgacg agtttttcat gcgaggttcg acgatccaag
    11401 gcgcaaagac atttgcgcga tctgcttggg ccgacacgct tcactctagc gctttgcttt
    11461 tgacaagacg atgcgccagg tgccttttcg cttggcttgg cttgcggcga gcgacttggg
    11521 cttgaaaccg ggggcgctga cctcgcactc tccatcgccg gggatattca ccgtgcaggt
    11581 tgggctgcat cccagcgttt cgctaccgca tgtcactgtc gcaagcgctt tcttgggcga
    11641 catcgacagc ttgacccgga tgggcggctt gctgaccggc ggcgtgacga cgggcggcga
    11701 cttcacggtc ggatcgatcg cctgtccaga cttgccggcg tttggatcat cggcgaggct
    11761 tccggcgtct ttcggcgatg gcgcatgtcc cagatcgacg gcagcttcgg acttgggtcg
    11821 tgtcgtcgat aggtccagca ccagtgtcgg tggcggcgtc ggaccatcgc tgcgcagcac
    11881 aaagaagatc gtcgcggcga gcgtacccaa aatcgcgatc ccacccaccg ccaacaaaat
    11941 gcgcggcaga cgggacgcgg gcggcggcag caccactttg gcgttgctgc tccccagcag
    12001 cacttgctct atcgcactca gcgcgtgctc gacgtctgac attttttcga agcggtctgc
    12061 cggctttttt gccatgcagc gcgcaatcac ctgctccagc gcttcgggga tgtgggcttc
    12121 gggacgaact ttgcgcggcg gaatcggctg tttgtagacg tgcttgtaca ccacgtcggt
    12181 cgtcgtcggc ccgtcgaagg gcagaatcga cgtcagcatt tcatacaaaa tgcagcccag
    12241 cgcatactga tcgcttcgtg cgtcggtgtc gcgtcccgtg acttgctcgg gcgaaatata
    12301 ttccgccgtg ccgacgacgg tgccatcctg cgtcagtcgc tgcccctcgt cgagcttcgc
    12361 cagtccaaag tcgacaatct tcaccacatc ctgtctcccc tcccgctcga ggagaaa
//
LOCUS       DAORGN010000150        12147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2189_length_12147_cov_5.760999, whole genome shotgun sequence.
ACCESSION   DAORGN010000150 DAORGN010000000
VERSION     DAORGN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_12147_cov_5.760999"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="PKL17_15295"
     CDS             complement(<1..1744)
                     /locus_tag="PKL17_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK46150.1"
                     /translation="MSGQLRFDGKVVIITGAGQGLGRSHALAFASRGAKVVVNDLGGA
                     ATGGGKSSAAADKVVAEIKAAGGEAVANYDSVEDGAAIVKTAIDAFGRIDVVVNNAGI
                     LRDTSFPKMTDQDWELIFKVHMLGAFRVTHAAWPYMRDQGYGRIIFTSSAAGIYGNFG
                     QANYGAAKLGLVGFANTLAVEGAKKNIRVNTIAPLAGSRLTETVMPKEVVDALKPEYV
                     SPVVLFLGHEECQETGALFEVGGGFCAKLRWERTKGKIWKLGRNITIENVRKAWGEIT
                     NFDGATHPENITASMQPVMSNLQSKSQGGNDLIDVDEALGFELPEQKSSYDERDLAIY
                     ALGVGAGKNSTDDKDLRFVYENNSAGFYPLPTFAVVPALKMLFKLAAEGTTAPGLHYG
                     LDRVLHGEQYTEVVRPWPAKAKLSHRARISEIYDKGKNAVVVTHIDTYDADSGDLIAK
                     NDISTFVRGGGGFGGERGPSSDVNNPPDRPADYVATDKLGDEQALIYRLSGDWNPLHV
                     DPTFATAFGFPKPILHGLCSFGYLGRHVVNHICGGDPRYFKSIKVRFADIVFPGETLK
                     IELWKESDTRVIARV"
     gene            2035..3600
                     /locus_tag="PKL17_15300"
     CDS             2035..3600
                     /locus_tag="PKL17_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46151.1"
                     /translation="MANIKVPEGLTLAPQNVPGGRYEDGWRQGKPVEDPAKMKRWGFI
                     TAKPSEYLVVVHNGKIDSRRSGQGMRVWKWPWTAVATIPTTLQQIEFVADQITKERVG
                     VSVTGMAVYRIAQPEIAYRMLNFSYAERASEKLGQTLRDMFIGAARRLIANLSLNQCL
                     EHRKEAIAAFLMQEISPVVSGSGRPDDTTDQGWGVIIDTIEIQDVKILSDAVFKHLQA
                     PYRAEIAMRAEQAELERQRQVSETRATAERQTQEAAITSQRETRISKAKAEAEAAERE
                     AQEAMRAEQAKAKGAAEELLRKEALARKRIEVEEGIALRQADSKASLDGKSQQIRQES
                     ELAQLKLQEQKRVASAQAEMATLATDAELAEKAHKLELRKLELEQARRQLRQKQELAL
                     HAEKAHFEQEQRKRESETVRLENEAVLAKQRAEAEIELLLSQGRVMRDLVVTGLPHIA
                     QAFKQSFGTINYTQYSGSPDGNPMAMVGQAIEQVLHVAKGFGLDPAKLGQPVAQVEAA
                     PPAASSEARVNKE"
     gene            3666..4634
                     /locus_tag="PKL17_15305"
     CDS             3666..4634
                     /locus_tag="PKL17_15305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNK46152.1"
                     /translation="MRYLVTGGAGFVGSHLSEELLRRGHEVIVIDDLSTGTIRNIRHL
                     TDNKRFRYVIDTVANRTLLAELVDDADVIYHLAAAVGVKLIVESPVRTIETNIKLTEL
                     VLEMAQKKKRPVFIASTSECYGKSTDFPFREEGDLVLGPSSKGRWSYACSKLIDEFLA
                     IAYHRERGLPTVVGRLFNTVGPRQTGQYGMVVPTFVKQALAGQPVTVFGDGQQSRCFT
                     HVADAVRALIGLMERPDAYGQVFNIGNTEEVSIFNLAQRVISQSGSRSEIQFVPYERA
                     YEPGFEDMNRRIPDVSKIGQLIGWKPTISLDQILGDVIAWCRTGAA"
     gene            4638..5441
                     /locus_tag="PKL17_15310"
     CDS             4638..5441
                     /locus_tag="PKL17_15310"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNK46153.1"
                     /translation="MELWQVVLLSVVEGLTEFVPVSSTGHLILLSRLLGLKGPAVDAF
                     LVVVQLGALLAAVVYYRKTIGETIVGIVQKRPAALLLFRNLILGSLPLLAVGYLLGKR
                     IKAQLFAPLPVALALGVGGLLMLLIERHRRRHAATFESASALPPKQALTVGLCHLFAL
                     WPGTSRSLASMSGALIAGLPTKAAADYAFLLAIPTLGTATVYELLKERHTLLAEVGLF
                     HMSVGLAVSFAVGWLVIAAFLRYLRGHGLGLLALYRVVLAAVVLWLWRF"
     gene            complement(5450..5935)
                     /locus_tag="PKL17_15315"
     CDS             complement(5450..5935)
                     /locus_tag="PKL17_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46154.1"
                     /translation="MKKLAWLIFGLFGVAGCDYTPLGMPSMPADLRPAGDLAGADLSA
                     PSYKATLPTCTPVTVTAGQVYSGVVSGSCISCHKTTAPKMATAQDLVNNLVGKMPRAA
                     QMPLVTAGDPNKSYLIFRLTGEGDLITGGSSGWMPSGSSMLGQSAMCLWVNWVRSGAP
                     P"
     gene            complement(6022..>7329)
                     /locus_tag="PKL17_15320"
     CDS             complement(6022..>7329)
                     /locus_tag="PKL17_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46155.1"
                     /translation="AKAGAKGALASAAKDAGAKVDAVKSAAKDAVGSLKSAAKDAVGS
                     LKSAAKDAAKDTAKPAAKDAAKEVPAAKAAPKADADDLLEDDALESVDAIEEDIAMPA
                     PPPKGSKPPPPKPPAMKAPPSLPKPPAPAPALAAPAPTPVAPPAPAPIPAAAPAPALA
                     EPPAPIAPPIAPPVAPSFDPAPAEAPVPAPGPQGLAPGEWPPELSGLPQPTSTDGGYS
                     GPGLSGMGGQPSGYDAGGNPYGGNPYGGNPYGGSPYGGSGDPNAAMGAAPDSASASPP
                     AKSKVPLFAAAAALVALLAGGGYFLLVKPPAGNAGALADAGTDAGTAKPKEEPKPAPK
                     PEPPKITGITKAEFDELWSAGSAPVLACFEKAIKKQGDLEGKELTISVEVSDKGKTGE
                     LTFAGPDLDEKTKKCIAKAMKKWKFPAKDKSAYTAKFPVKIAK"
     assembly_gap    7330..7429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7430..8145)
                     /locus_tag="PKL17_15325"
     CDS             complement(<7430..8145)
                     /locus_tag="PKL17_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNK46156.1"
                     /translation="MKFSCEQCQTKYNLPDERVRGKVLKIRCKKCGCQITVSQGGVRT
                     SRNDGDGEDATMVGSRASVAAMTGGKVPAGGGGGGGHDDGGEGGDSTMIGGMNDFFAN
                     LAGAGSQAQVPDEWHLSIDGNVVDPMPLADLAQRVVVELATPGRDMYVWREGQPEWLP
                     PDSIPEVQAAVEKAKRNPAPPKAAAKPAPAAAAGLGDEDEDEGGDKTQMGSLDFAALG
                     LEAKAPWEEAAAKSEPAKADA"
     gene            8435..9397
                     /locus_tag="PKL17_15330"
     CDS             8435..9397
                     /locus_tag="PKL17_15330"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNK46157.1"
                     /translation="MLTTAAWAKDPGCSPEVAAARRAVISAGRAVVSAPVVDLYERAD
                     QNGPVADQARLGELVTLVPQPEACAQKSDQLVLVETTAAYRGYVLLSQLVPWQKDQLA
                     ASAAPAQAGTTLAAGTRLRVSSRLANLFRSPDVTLANPRFVLPLHTELRLVEAQSPRW
                     LKVALPDGTAGFVQRGDVDVITQETRPPVSCFLQEARKYEGTPYLWGGRSTLGIDCSG
                     LVASAFLACGVSAPRDAGPQMTWADMLPVESQSTLKPGDLVFFRSGEPANASSMNVAH
                     VGIFVGEGRFFHATTSDRPTVHESNLGEPQWQRRYLGARRHRSF"
     gene            complement(9394..10392)
                     /gene="gmd"
                     /locus_tag="PKL17_15335"
     CDS             complement(9394..10392)
                     /gene="gmd"
                     /locus_tag="PKL17_15335"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNK46158.1"
                     /translation="MSTRRALITGITGQDGSYLAEFLLRKGYEVHGIVRRSSSFNTGR
                     LEAMYQDPHVADYRLKLHYGDMVDSSVLGRILHSVRPDEVYNLAAQSHVRVSFDMPEY
                     TSDVVAQGTTRLLEVLRELGPNTRFYQASSSEMFGASAPPQSETTPFRPCSPYAIAKV
                     AAHQTTGLYREAYGLFAACGILFNHESPRRGETFVTRKVTRAAAKIKLGMQDKLFLGN
                     LEARRDWGFAGDYVEAMWLMLQIDKPEDLVICTGESHSVRELLEVAFGRLDLDWERYV
                     EIDPRYFRPSEVDDLRGDMTRARKVLRWEPKVRFRQLVEMMVDHDLADLLRHRKSR"
     gene            complement(10389..11327)
                     /locus_tag="PKL17_15340"
     CDS             complement(10389..11327)
                     /locus_tag="PKL17_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNK46159.1"
                     /translation="MDLSKKRIVVTGGAGFLGSFVVEALTARGCQNVFVPRSREYDLV
                     DMSAVRRLLDDARPDVIIHLAARVGGILANQQNPGRFFYDNLMMGVQLLDESRRFGVE
                     KFVATGTICAYPKHTPVPFREDDLWNGYPEETNAPYGLAKKMLLVQSQAYRAQYGFSS
                     VVLFPVNLYGPRDNFDLTTSHVIPALIRKVVEAMRAGRDEVPIWGDGTPTREFLYVAD
                     CAEGIVLATERFDDSQPVNLGSGQEISIATLAQQIGNILGFRGRFVYDQSRPNGQPRR
                     ALDCSRAEKLFGFRATTGFETGLRRTIDFYLRELST"
     gene            complement(11333..>12147)
                     /locus_tag="PKL17_15345"
     CDS             complement(11333..>12147)
                     /locus_tag="PKL17_15345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNK46160.1"
                     /translation="LLLPLDLSMLAYVAMMAPRLVELHRVLKPSGSLYLHCDTSAGAY
                     LKLLCDAIFGGECFLNEIVWSYRRWPSRTRYFQRMHDVIYLYARQPGPGRTFHVEYEP
                     LSDSYRKRFGGSTQVLDPAHPSRKRKLDEKSKGMPQRDVWDIPILSGSSHERVGYPTQ
                     KPIQLIERIIRASSNPGDVVLDPFCGSGTTLVCAEALGRRWIGIDIAPFGIAHTRRRL
                     LHRFELLLSPYDFVAHPESLPHVDALSQKLGPLRSPARTTTTRRRSSSKESK"
BASE COUNT         2179 a         3641 c         3844 g         2383 t
ORIGIN      
        1 tgacgcgggc gatgacgcgg gtgtcggact ctttccacag ctcgatcttg agcgtttcgc
       61 cggggaatac gatgtcggca aagcgcacct tgatgctctt aaagtaccga gggtcaccgc
      121 cgcagatgtg gttgacgacg tggcggccca gatatccaaa tgagcacagg ccgtgcagga
      181 tcggtttggg gaagccaaac gccgtcgcaa acgtcgggtc gacgtgcagc gggttccagt
      241 cgccgctgag ccgatagatg agcgcttgtt catcgccgag cttgtcggtg gcaacgtagt
      301 cggcgggacg gtcgggcggg ttgttgacat cgctcgacgg gccacgctcg ccaccaaagc
      361 cgccaccgcc gcgcacgaac gtgctgatgt cgtttttggc gatgagatca ccgctgtccg
      421 cgtcgtaggt gtcgatgtgg gtgacgacga cggcgttctt gcctttgtcg tagatttcgg
      481 aaatccgggc gcggtggctc agctttgctt tggccggcca cggacggacg acttcggtgt
      541 actgctcgcc gtggagcacg cgatcgagtc cgtagtgcag gcccggcgcg gtcgtgccct
      601 cggcggccag cttgaagagc atcttgagcg caggcaccac cgcgaacgtc ggcagcggat
      661 agaagccggc agaattgttt tcgtagacaa agcgcaggtc tttgtcgtcg gtcgagttct
      721 taccggcacc gacgccgagt gcgtagatgg ccaggtcgcg ctcgtcgtag ctgctcttct
      781 gttcgggcag ctcgaaaccg agcgcttcgt cgacgtcgat cagatcgttg ccaccctggc
      841 ttttcgactg aaggttggac atcaccggct gcatcgacgc ggtgatgttt tccggatggg
      901 tggcaccgtc gaagtttgtg atctcgcccc acgctttgcg gacgttttcg atggtgatgt
      961 tgcggcccag cttccagatt ttgcccttgg tgcgttccca gcggagcttg gcgcagaagc
     1021 cgccaccgac ttcgaacagc gcgccggttt cctggcattc ctcgtggccc aggaacagca
     1081 cgaccgggct tacgtattcg ggcttgagcg cgtcgacgac ttctttgggc atgaccgttt
     1141 cggtcaggcg cgagccggca agcggcgcga tggtgttgac gcggatgttc tttttggcac
     1201 cttcgacggc gagggtgttg gcaaaaccga cgagcccgag ctttgctgcg ccgtagttgg
     1261 cctggccgaa gttgccgtaa atgccggctg ccgacgacgt aaagatgatg cggccatagc
     1321 cctgatcgcg catgtacggc cacgccgcgt gggtgacgcg gaaggcaccg agcatgtgga
     1381 ctttgaagat cagctcccag tcttggtccg tcatcttggg gaagctggta tcgcgcagga
     1441 tgccggcgtt gttgacgacg acgtcgatgc gaccaaaggc atcgatcgcg gtcttgacga
     1501 tcgcggcgcc gtcttcgaca gagtcgtagt ttgcgacggc ctcgccgcct gccgctttga
     1561 tttcagcgac gactttgtcg gctgcggcgg acgacttgcc gccgccggtt gccgctccgc
     1621 cgaggtcatt gacgacgact ttggcgccgc ggctggcaaa cgcgagtgca tgactgcggc
     1681 ccaggccctg cccagcgccg gtgatgatga cgacttttcc gtcgaagcga agctgtcccg
     1741 acatctgtct tctactccac ttgctgtctg aaagattgac tcatccatgt accccctgac
     1801 gggcggtgaa gtcaaaccct tggcgtcgac tcaaggtgat ccattttggc gcaggtgctg
     1861 ggcaggtgat ccattttgga acaggggatg gcgcaggccg agcacaggcc accgggcgaa
     1921 gcgaccccaa agcggcagaa agcgcagccg tttcgcggac tttttcgtcg gccattgtgg
     1981 ttggtacggc ggctgcattc tccttgttgg cagaaggaaa ggagctttcg catcatggcc
     2041 aacatcaaag tgcccgaagg tcttacgctt gccccccaaa acgttcctgg ggggcgctac
     2101 gaggatggct ggcggcaggg taagcccgtc gaagaccccg ccaaaatgaa gcgctggggc
     2161 ttcatcaccg ccaagccgag cgaatacctg gtcgtcgtcc acaacggaaa aatcgacagc
     2221 cgccgatcgg gccagggcat gcgggtgtgg aagtggccat ggaccgcggt ggcgacgata
     2281 ccgacgacgc ttcagcagat cgagtttgtc gccgaccaga tcaccaaaga gcgcgtcggc
     2341 gtcagcgtca ccggcatggc ggtctatcgc atcgcccagc ccgaaatcgc gtaccgcatg
     2401 ctcaacttca gctacgccga gcgggccagt gaaaagctgg gacagacgct gcgcgacatg
     2461 ttcatcggtg cggcacgacg gctgattgcg aacctttcgc tcaaccagtg tctggagcat
     2521 cgcaaagagg cgatcgcggc gtttttgatg caagaaatct cgccggtggt atcgggcagc
     2581 gggcgtcccg atgacaccac ggaccaaggc tggggcgtca tcatcgacac catcgaaatt
     2641 caggacgtga aaatcctgtc tgacgcggtg ttcaagcact tgcaggcccc gtatcgggcc
     2701 gaaattgcga tgcgcgccga gcaggccgag ctggagcggc agcgccaggt gtccgagacg
     2761 cgcgcgacgg cggaacggca gacccaggaa gcggcgatca ccagccagcg tgaaacgcgg
     2821 atctcgaaag cgaaagccga ggcggaagct gccgagcggg aagcgcaaga agcgatgcgc
     2881 gccgagcagg cgaaggccaa aggcgctgcc gaggagcttt tgcgcaaaga agcgctggcc
     2941 cgcaaacgca ttgaagtcga agaaggcatc gcgctgcggc aggctgacag caaggcatcg
     3001 ctcgacggca agagccagca gattcggcag gaatccgagc tggcgcagct caagctgcaa
     3061 gagcagaagc gcgtggcatc ggcacaagct gaaatggcga cgctcgcgac cgacgcggag
     3121 ctggccgaaa aagcccacaa gctggagctg cgcaagctgg aactcgaaca ggcgcgacgg
     3181 cagcttcggc agaaacagga acttgcgctg cacgccgaaa aagcgcactt cgagcaggaa
     3241 cagcgcaagc gagagtccga gacggtgcga ctcgaaaacg aagcggtttt ggccaagcag
     3301 cgggccgagg cagagatcga gcttttgcta agccaaggac gggtgatgcg tgacttggtg
     3361 gtgacggggc tgccgcacat cgcgcaggcg ttcaagcagt cgtttggcac gatcaactac
     3421 acccaatatt cggggtctcc cgacggaaac ccgatggcaa tggtgggcca agcgatagaa
     3481 caagtgctgc atgtggccaa aggctttgga ctcgatccgg ccaagctggg acagccggtg
     3541 gcccaggttg aagcggcccc accggcggct tcgtcggaag cccgagtgaa caaagaatag
     3601 gctgggtttt ccgacggacc aatcgcggat tgtcttgctg ctgcggctgc gctaacgtcg
     3661 cgaaaatgcg ttacttggta actggcggcg ccggctttgt cggctcgcat ctatcggaag
     3721 agcttttgcg gcgcggccat gaagtgatcg tcatcgacga tttgtcgacg ggaacgatcc
     3781 gcaacatccg ccacttaacc gacaacaagc gctttcgtta cgtcatcgac accgtcgcca
     3841 atcgcacgct tttggcggaa cttgtcgacg atgccgatgt gatctatcac ttggccgctg
     3901 ccgtcggtgt caagctgatc gtcgaaagcc cggtccgcac catcgagacg aacatcaagc
     3961 tgaccgagct ggtgcttgaa atggcgcaga agaaaaagcg cccggtcttc atcgcttcga
     4021 cgtcggaatg ttacggcaaa agcaccgact ttccgtttcg cgaggaaggc gatctggtgc
     4081 tcggtccgtc gtcgaaaggc cgctggtcgt atgcctgcag caagctgatc gacgaatttt
     4141 tggccatcgc gtaccaccgc gagcgtggcc tgcctaccgt cgttggacga ttgtttaaca
     4201 ccgtcggacc gcgccagact ggccagtacg gcatggtcgt tccgacattt gtaaagcaag
     4261 cgctcgccgg gcaacctgtg acggtgtttg gcgacgggca gcagtcgcgc tgttttaccc
     4321 acgtcgcaga tgcggtgcgg gcgctgattg gtcttatgga acgtcccgac gcctacggcc
     4381 aagtcttcaa catcggcaac accgaagaag tcagcatctt caacctggcc cagcgcgtca
     4441 tttctcagtc gggcagccgc agcgaaattc agtttgtccc gtatgagcgc gcctacgaac
     4501 ccggcttcga ggacatgaac cgccgcattc ccgacgtcag caagatcggc cagcttatcg
     4561 gctggaaacc gacgatctcg ctcgatcaaa tcctgggcga cgtcattgcc tggtgccgca
     4621 ccggcgcagc ctaagccatg gagctgtggc aagttgttct tctatcggtc gtcgagggtc
     4681 tgaccgagtt tgtgccggtg tcatcgacgg ggcacctgat ccttttgagc cgcctgttgg
     4741 ggctcaaagg gccggcggtc gatgcgtttt tggtcgtcgt ccagctcggc gcgctgcttg
     4801 ccgcggtggt ctattaccga aaaacgatcg gagaaaccat cgtcggaatc gtccaaaaac
     4861 ggcccgctgc gctgcttttg tttcgcaacc tgattttggg gtcgcttccg cttttggccg
     4921 tcggatatct gctcggtaag cggatcaaag cgcagctttt tgcaccactg cccgtcgcgc
     4981 ttgcacttgg ggtgggcggg cttttgatgc tgcttatcga acgccatcgc agacgccacg
     5041 cggcgacgtt tgaaagcgcg tcggctctgc cgccaaaaca agcgctgacc gtcgggctgt
     5101 gtcacctttt tgcgctgtgg ccgggcacgt cgcggtcgct tgccagcatg agcggggcgc
     5161 ttatcgccgg gctgccgacg aaagccgccg cagattatgc gtttctgctg gcgatcccga
     5221 cgctgggcac cgcaaccgtc tacgaactgc tcaaagagcg ccacaccctg ctcgccgagg
     5281 ttgggctgtt ccacatgagc gttggtctcg ccgtctcatt tgccgtggga tggctggtga
     5341 tcgcggcgtt tttgcgttac ctgcgcggcc acggacttgg actcttggcg ctttatcgcg
     5401 tcgtgctcgc cgccgtggtg ctgtggctgt ggcgcttcta gagcgcgcgt tatggcggtg
     5461 cgccgctgcg aacccagttg acccacaggc acatcgcgct ctgtcccagc atcgaactgc
     5521 ctgacggcat ccagccggag ctgccaccgg taatcagatc gccttcgcca gtcagacgga
     5581 agatcagata cgacttgttg ggatcgcccg ctgtgacgag cggcatctgc gccgcgcgcg
     5641 gcatcttgcc gaccaggttg ttgaccagat cctgcgcggt ggccatcttc ggcgctgtcg
     5701 ttttgtgaca gctaatacac gatcccgaga cgacgccgga atagacctgg cccgcggtga
     5761 cggtgactgg cgtgcaggtc ggcagggtgg ccttgtaact cggtgctgac aggtccgcac
     5821 cggccaagtc gcccgctggt cgcaaatccg ccggcatcga gggcattccc agcggtgtgt
     5881 agtcgcaccc agctacacca aacaggccaa aaataagcca agctagcttc ttcacgtctg
     5941 tcctccccca gagagtgaag tgaccaaggc ataaccagtc catcatagga caggcggacc
     6001 gaatctctca caagggaagc tttactttgc gattttgaca gggaacttcg cggtgtaagc
     6061 ggacttgtct ttggccggga acttccactt tttcatcgcc tttgcgatgc actttttggt
     6121 cttttcatcc agatcggggc cggcaaatgt cagctcgccg gtcttgcctt tgtcggacac
     6181 ttcgacggaa atcgtcagct ctttgccttc cagatcgccc tgcttcttga tcgctttttc
     6241 aaagcaggcc aaaaccgggg ccgatcctgc cgaccacagc tcgtcgaact cggccttggt
     6301 gatgccagtg atcttcggcg gctccggctt gggcgctggc ttgggttctt cttttggctt
     6361 ggccgttccc gcatcggttc cggcatccgc aagagcaccc gcgttgccag caggcggttt
     6421 gaccagcagg aaatacccac cgccagcgag caaggcgaca agggccgccg ccgccgcaaa
     6481 cagcggaact ttcgacttgg ctggcggtga cgcgctggca gaatcggggg ccgcgcccat
     6541 cgctgcattg ggatcgccac tgccgccgta aggacttccg ccataggggt tgccgccata
     6601 cggattgccg ccgtagggat tgccacccgc gtcgtaaccg cttggctgtc cacccatgcc
     6661 gctcagtcct ggtccgctat agccaccatc ggtggatgtc ggctgcggca gccctgatag
     6721 ctcgggcggc cactcgccgg gggccaggcc ttggggtccg ggcgctggga cgggggcttc
     6781 tgctggcgcg ggatcaaagg atggtgcgac aggcggcgca atcggcggcg caatcggcgc
     6841 cggtggttcc gccagggcgg gcgctggcgc ggcagccgga atcggcgcgg gcgcgggcgg
     6901 tgccacaggc gtcggggccg gggccgccag ggccggagct ggtgccggtg gcttgggcag
     6961 cgacggcggt gccttcatcg cgggaggctt cggcggtggc ggcttgctgc ctttgggcgg
     7021 cggagccggc atcgcgatgt cttcctcgat cgcatcgacc gattcgagcg catcgtcttc
     7081 cagcaggtcg tccgcatcag cctttggtgc tgctttggct gccggaacct ctttggccgc
     7141 gtcttttgct gccggcttgg ccgtgtcttt ggctgcatct tttgccgcgg acttgagcga
     7201 accgaccgcg tcttttgcgg cggacttgag cgaaccgacc gcatcttttg ctgccgactt
     7261 gaccgcatcg actttggcgc cggcgtcctt ggcggctgat gccagtgcgc ccttggctcc
     7321 ggctttcgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgtccgcttt
     7441 ggcgggctct gacttggccg cggcttcttc ccacggcgct ttggcttcca ggccgagcgc
     7501 cgcaaagtcg agcgatccca tctgggtttt gtcgccgcct tcgtcttcgt cctcgtcacc
     7561 gagtcctgct gcggctgccg gcgccggctt tgcggcagct ttgggtggcg cgggatttcg
     7621 cttggctttt tcgaccgccg cttggacttc cggaatcgaa tcgggcggca accactcggg
     7681 ctggccttcg cgccagacgt acatgtcacg gcccggcgtc gccaattcca cgaccacgcg
     7741 ctgtgccaga tcggcaagcg gcatcggatc gaccacattg ccgtcgatcg acaagtgcca
     7801 ctcatccgga acttgggcct ggctgcctgc cccggcgagg ttggcgaaaa agtcgttcat
     7861 tccgccaatc atcgtcgagt cgccgccctc accaccgtcg tcgtggccgc cgccgccccc
     7921 accgcccgcg gggactttgc cgccggtcat cgccgcgacc gacgcgcgcg agccaaccat
     7981 cgtggcgtct tcgccgtcgc cgtcgttgcg agacgtccgc acgccgcctt ggctgacggt
     8041 gatctggcaa ccacacttct tgcagcgaat tttcagaacc ttgccgcgaa cgcgctcgtc
     8101 gggaaggttg tatttggttt ggcactgttc gcaggagaac ttcatggtcc gggggctccc
     8161 gacgcgagcg attctgtgtt tgaccttacg gggtgggcag aagcgggccg ccgccgtccc
     8221 caagctctag acatctgcag tgacacgact cgacgatcgg ttctcactat acccgaagaa
     8281 agaaaggcaa aaaagccctg tcagttcgca tattttccgg agtcttcatg acatgaaacc
     8341 caagtgcccg tgctagcttc cgggcgtgac acatcgcgtc tgcttgcgga aatggagtgg
     8401 tcgggggcgt cttttttgtg cgggcctgct ggttttgctg acgaccgcgg cctgggccaa
     8461 ggatcctggc tgcagcccgg aggtcgcagc ggcgcggcgg gcggtgattt cggcaggtcg
     8521 ggcggtggtg tcggccccgg tcgtcgatct gtatgagcgt gcggatcaaa atggcccggt
     8581 cgcggatcag gcgcggcttg gcgagcttgt gacgctggtc ccccagccgg aagcttgcgc
     8641 acaaaaaagc gatcagctcg tcttggtgga aacgacggcg gcgtatcgag gctacgtgct
     8701 tttgtcccag cttgtgccgt ggcaaaaaga ccagcttgcg gcgtcggcgg cgccggccca
     8761 agcgggcacg acgctggctg cgggcacgcg gctgcgggtg tcgtcgcgcc tggccaactt
     8821 gttccgcagt cccgacgtga cgctggccaa tcctcgcttt gtgctgccgc tgcacaccga
     8881 gcttcggctt gtcgaagcgc agtcgccgcg ctggctgaaa gtggcgttgc ccgacggcac
     8941 ggctggcttt gtgcagcgcg gtgacgtcga tgtgatcacg caggaaacaa ggccgccggt
     9001 gtcttgcttt ctgcaagaag cgcgcaagta cgaagggacg ccgtatttgt ggggtgggcg
     9061 ctcgacgctg gggattgact gttcggggct tgtcgccagc gcgtttttgg cgtgtggcgt
     9121 gagcgcgccg cgtgacgccg ggccgcagat gacctgggcc gacatgttgc ccgtcgaatc
     9181 acagagcacg ctaaagcccg gtgatttggt gtttttccgc agcggtgagc cggcaaacgc
     9241 cagttcgatg aacgtcgccc acgtcggcat ttttgtcggc gaaggtcgtt ttttccacgc
     9301 gacgacgagc gaccggccca ccgttcacga aagcaacctg ggcgaaccac agtggcagcg
     9361 ccgatacttg ggcgctcgtc gacatcgcag cttttagcgg ctctttcggt ggcgcaaaag
     9421 atccgccaga tcgtggtcga ccatcatctc gacaagctga cggaagcgga ccttgggttc
     9481 ccatcgcagc accttgcggg cccgcgtcat gtcgccgcgc agatcgtcga cttcgcttgg
     9541 gcgaaagtag cgcggatcga tttcgacata gcgttcccag tcgaggtcga gtcgaccgaa
     9601 ggccacttcc agaagctcgc ggaccgaatg gctttcgccg gtacagatca ccaagtcttc
     9661 gggcttgtcg atctggagca tcagccacat cgcctcgacg taatcgccgg caaaacccca
     9721 gtcgcgccgc gcttccaggt tgcccaaaaa cagcttgtcc tgcatcccca gcttgatctt
     9781 ggcggcagcg cgggtcactt tgcgggtcac gaaggtttcg ccgcgacgcg gactttcgtg
     9841 gttaaaaagg atcccgcagg cggcaaacag accataggct tcgcgataca ggccggtggt
     9901 ttggtgtgcc gcgactttgg caatcgcata cggtgaacac ggacgaaacg gcgtggtttc
     9961 cgactgcggc ggcgcggatg ctccgaacat ttccgacgat gacgcctgat aaaaacgggt
    10021 gtttggtccc agctcgcgca gcacttccag aagccgggtc gtgccctgcg cgacgacatc
    10081 gcttgtgtat tccggcatgt cgaacgacac ccggacgtgg ctctgggctg ccaggttgta
    10141 gacttcgtcg ggacgcaccg aatgcaaaat ccgcccaagc accgacgagt ccaccatgtc
    10201 gccgtagtgc agcttcagcc ggtaatcggc aacgtgtggg tcttggtaca tcgcttcgag
    10261 ccgcccggta ttgaacgacg atgagcgacg gacgatgccg tggacttcgt agcctttgcg
    10321 gagcaaaaac tccgccagat aagagccgtc ttgcccagtg atgccggtga tgagtgcgcg
    10381 gcgggtgctc atgtcgatag ctcgcgaaga tagaagtcga tggtgcggcg cagcccggtt
    10441 tcgaagcccg tcgtagcgcg aaagccaaac agcttttcgg cgcggctaca gtcgagcgcg
    10501 cgacgcggct gaccgtttgg ccggctttgg tcatagacga agcggccccg gaaacccagg
    10561 atgtttccga tctgctgcgc cagggtcgcg atcgagattt cctgacccga tccgaggttg
    10621 accggctggc tgtcgtcgaa gcgctcggtc gcaaggacga tgccttcggc gcagtctgcc
    10681 acgtacaaaa actcgcgcgt gggtgtgccg tccccccaga tcggcacttc gtcgcggcct
    10741 gcgcgcatcg cttcgaccac tttgcggatc agcgccggaa tgacgtgcga tgtcgtcaga
    10801 tcgaagttgt cgcgtggccc atacaggttg actgggaaca aaaccaccga cgaaaaaccg
    10861 tactgggcgc ggtaggcctg cgactgcacg agcagcattt ttttcgccag cccgtaaggc
    10921 gcattggttt cctcggggta gccgttccac agatcgtctt cgcgaaacgg caccggcgtg
    10981 tgctttggat acgcgcaaat cgtcccggtg gcgacaaact tttcgacccc aaagcgacgc
    11041 gattcgtcga gaagctgcac gcccatcatc aggttgtcgt aaaaaaatcg tccggggttt
    11101 tgctggttcg ccaaaatccc gccgacgcgc gccgccaggt ggatgatcac gtcgggccgc
    11161 gcatcgtcaa gcagccgccg cacggctgac atatcgacca agtcgtactc gcgtgagcgc
    11221 ggcacaaaca cattctggca gccacgggcg gtcagggctt cgacgacaaa cgatcccaaa
    11281 aaaccggcgc cacccgtgac gacaatgcgc tttttggaca ggtccatcgc ggtcatttgc
    11341 tttccttgct gctgctgcgt cgtcgtgtgg tggtcgtccg cgctgggctg cgcagcggtc
    11401 caagcttttg tgaaagcgca tcgacgtgcg gaagtgattc ggggtgcgcc acaaagtcat
    11461 acggggacag caaaagctcg aaacgatgca gaagccgccg tcgggtgtgc gcgatgccaa
    11521 acggggcgat gtcgatgccg atccagcgcc gtcccagcgc ttcggcgcag accagcgtgg
    11581 tccccgaacc acagaacgga tcgagcacca cgtcgccggg attgctcgat gcgcggatga
    11641 ttcgctcgat aagttgaatc ggtttttgcg tcggatagcc gacccgctca tggctcgatc
    11701 ccgacagaat cggaatgtcc cagacatcgc gctgcggcat gcctttgctt ttttcgtcca
    11761 gcttgcgctt gcgcgaagga tgcgccgggt cgagcacctg cgtcgatccg ccaaagcgct
    11821 tgcgatagct gtcgctcagc ggttcgtatt cgacgtgaaa cgtccgcccc ggccccggct
    11881 gccgcgcgta caaatagatc acgtcgtgca tccgctgaaa atagcgcgtc cggctcggcc
    11941 agcgtcgata gctccagacg atttcgttca aaaagcactc cccgccgaag atcgcgtcgc
    12001 acagaagctt cagatacgcc cccgccgacg tgtcgcagtg caggtacagg ctgccgcttg
    12061 gctttagcac ccggtgcagc tccacaagcc gcggcgccat catcgccaca tacgccagca
    12121 tcgacagatc gagcggcaag agcagcg
//
LOCUS       DAORGN010000151        12141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2191_length_12141_cov_6.060235, whole genome shotgun sequence.
ACCESSION   DAORGN010000151 DAORGN010000000
VERSION     DAORGN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_12141_cov_6.060235"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKL17_15350"
     CDS             complement(<1..511)
                     /locus_tag="PKL17_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNK46161.1"
                     /translation="MHLASTGQPSETMSQHSPNDCWVPANDQPSVLLSPLLSSLSVRH
                     GFTTRQGGFSQGRFASLNLGRNWGDEPGCVSRNFARVATIGGFDLARLSQVRQVHGAK
                     VLHLDSPELARREADGMVTTHDLVLGVLSADCVSLLVADDEGRVAAAHAGWRGTVSGI
                     ATEVVATMAT"
     gene            complement(526..1656)
                     /locus_tag="PKL17_15355"
     CDS             complement(526..1656)
                     /locus_tag="PKL17_15355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNK46162.1"
                     /translation="MTESQDPIVFTVPPSAAGLRLDVYLAQQELPFSRSQLARRIEAG
                     EVTLDGRACRPGQKLRSGQTLRFVPSPPAPTHDEPEDIPLTVLYEDRHLIVIDKPAGM
                     VVHPAPGHETGTLVNALLHHCGSLPKPLGRPLADEDGDEDEVDAETDEPSTDRKAGAG
                     AATAIQHAPLSIGGERRPGIVHRLDSGTSGVLVCAKDEPTLVGLQVQFQAHSIERRYV
                     ALVDGVISERGTLSTRYGRHPRDRKRFTSRGGSKKAVTHYQVLERLPGATLVELRLET
                     GRTHQIRVHLSEQGFPILGDPLYGRPSRQRNVQIVSAQLTHQALHARLLGFVHPITGE
                     RLSFASAPPADFLSALTSLRGRADSPLLPWNAVGSRPASVHP"
     gene            complement(1653..1916)
                     /locus_tag="PKL17_15360"
     CDS             complement(1653..1916)
                     /locus_tag="PKL17_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46163.1"
                     /translation="MALIDKPERARQLARAISSDLTLYHEKEIIEGIENDTLFNVMHD
                     HIEEGRTLFKSRVTPDIYELGLFDRAIVDVMLKSKGHVKSRIW"
     gene            2077..4446
                     /locus_tag="PKL17_15365"
     CDS             2077..4446
                     /locus_tag="PKL17_15365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNK46164.1"
                     /translation="MTSLQEIPVSLDAETQKRYLSYALSVITSRALPDVRDGLKPVQR
                     RILYAMYHDQRLLPDAKFRKCAAVVGDVLGKYHPHGDASVYEALVRMAQDFSLRYPLV
                     DGHGNFGSQDGDAPAAYRYTECRLRATALLLLKELGQSTIDWRPTFDGVRSEPIVLPS
                     QLPNLLVNGVQGIAVGMATSIPPHNAGEVIDACLALIDEPELDSKSLLRYVRGPDFPT
                     AGELLTSKQELQEVYETGHGTLKLRATYHVEEPADRREGRRIIVNAIPYGVVRSAIME
                     KIGELFEEKKLPMVVDCRDESTTDVRIVIELKRGADPQLVMAFLFKNTALATTVQFNM
                     TCLVPTENPEIGTPKQLGLREMLRHFLDFRFQVVRRRLSHQLAELNKRIHVLDGFATV
                     YDALDEILAMIRRSEGKADAKEKLISRFKLDDDQAEAILELKLYRLAKLEILVIQEEL
                     SQKRKERAQLESLLQSDGKRWELVRRELGTLRDELADKRRTRIGVPAEEPAFDETAFI
                     AHEDCLVVLTRDGWVKRVGQLRDVSSTRVREGDEVQVVLGGSTKELAVFFSSRGSAYV
                     CRILDIPASSGYGEPLTKQFKFDDGETVVAALSLDPRVRPAADTHLVAVTRGGLAVRF
                     ALDAHAEVSTRNGRLFAKVSDGDGVLGVRVARGDALLTVATSLGRSLTCRVDEIPVLQ
                     GPGKGVQMLKLGEGDRMIGFAVGETLKVETDKGATHELGGSAADVTARGGKGREVLKK
                     GKLVRCLFAPPTVPQLTAKVVSAQAAQGDAPSEATPIPDIIGPLFRRPS"
     gene            complement(4484..5914)
                     /locus_tag="PKL17_15370"
     CDS             complement(4484..5914)
                     /locus_tag="PKL17_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46165.1"
                     /translation="MKRDFPLRGASRRDFIKGVMAASAALGLGPTRALELLDDIGGSA
                     LAAGAINANRTVHILGGTGGLAWFSMVWPVPGTITEFQTNPSVPWDDMAKAGKITSGA
                     LEDRTLYHRKVNGQPLWSSYGDKKLVTAMLCGTNTGHEVAPKNAGNSNTIPTGTIGSF
                     AACASIQTTVQSLVPVIGINKDAVTMPYGTATGSPSPASVPDYMSMINLFSSSASKLA
                     ARLSDPNNAKLYEEYYKAFLGLVKTVDRATYQRAYEDSRVAISLVSQNLGSVLAPVAG
                     QADAWCGPASIDANVKAIANNLIVAANAFKKGLCSQVMMPAFNDDPHFVWSDQARVTR
                     ISDGLALVFQSFMDYLNGIPDPVDPSKKLSDNLVMTVSGDLPKGPFSNKAADWTDSTP
                     GASNWMYVMSNGYMKPGWFGQVSRTGKMNYDPDSGKLYTPAQFPDSMVTPGALGAMLY
                     AVSRGNDRRVRDFTTAPYRAMMNVVL"
     gene            complement(5930..6649)
                     /locus_tag="PKL17_15375"
     CDS             complement(5930..6649)
                     /locus_tag="PKL17_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46166.1"
                     /translation="MKRKSGFFVRLGLVSMLGLIGCQNAASTPDPTMDPTQPTDVTQP
                     PVSPNTSTKSFDHDDILGNIGNPFADGRQVLPSDGQRMHVCGKIMYQALGTILTTRGI
                     NIANPAADSAGVLYQGGANIMGVANYPARVAEADRNSTGGLTRMFDMMLAVAEELIPT
                     ATTDRIGTGTACPGATLFNADNTCNADGFACLLGSPLTTVQLTECSNGVKRIASLTSN
                     DIMRAKRMTVATIASSAYLCD"
     gene            complement(6675..7883)
                     /locus_tag="PKL17_15380"
     CDS             complement(6675..7883)
                     /locus_tag="PKL17_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46167.1"
                     /translation="MTVSSMKKHIVPLLLAALPACSGGATSTDTAADPTGGFRATLPP
                     SVADTDKLALQDGKLDYNLALRSAALKLTGNYPTLAEIKQLQTATDQTTAYSQIIDTY
                     ISRPTFNQQILKFWKDTFKMGGMLPVTLPTGVVNVNLDFAPTYATFLVAKGQPFTNLL
                     TADTGTCQTYNAGTDSFTQSTCTNPAAVGILTDQGVQAQFYSAMAFRRVRWFQEALLC
                     SRFPAETNGPKVQFAGGVYNSPWDIKTVTGLGTDPKALVDFQDTSSLICANCHSTINH
                     QSPLFANFGADGIMKATIQVVTPVAGNPTSKLSDWLAAGEPLSYRFGQPTPDIKSFGA
                     AMAADPAFKACMATRVWNWAMSRGDVVVDQAILTPDFEAQLTQQIDNNKWNLKEFIRG
                     VYKSAYFIRY"
     gene            complement(8001..9101)
                     /locus_tag="PKL17_15385"
     CDS             complement(8001..9101)
                     /locus_tag="PKL17_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46168.1"
                     /translation="MLGALGLALGCAPPIITKDTGNSNNTSDEVLDGRGLFEYRVKSE
                     LVASCSCHSAGVNGFSPFLATGSEYASITGYRGGLFVPSEASTSPLLTKGAHAGPALT
                     VDQTNIVKGWLDVEATERGASGNSPTTPAVPFIVGDYYISLRALTQDPLARITFSMQL
                     ISGRTFRLTNLQLTAGPGGGIKIKHPRLVYITAGGATLEPSDALSTVDVTADAGKTVT
                     IGSGAILLPNVPASSSLVAFIFEQINVVNPMPVNVACKSLNLFTPPVTNTLAICASQC
                     HSAVGTRAEASRATRAFPMDAALKTDQTALASLCVDALGRINVTTPDKSILVQYVQPP
                     TQGGIASHVFKLDDATFTSFKAAVSAWAAAEK"
     gene            9481..10788
                     /locus_tag="PKL17_15390"
     CDS             9481..10788
                     /locus_tag="PKL17_15390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46169.1"
                     /translation="MPPSNPKQPPAKTAEGAAGKAQDRAPRIGEVLADNYRLVREIGR
                     GAMGAVYLAEDLALERQVAVKVLLPEHAVHPGQAALFRREARLLAAVRHENVVHVHTL
                     GEHRSIPYMVMEYIVGPTLAYYLVQAVRVHDAIPLDLAISLALQLCRAVQVVHAAGII
                     HRDIKPANVMVAPGPRAILMDFGLAKSTDSRGSGTHIIGTPDYLAPEQIRQRPRTPEE
                     ARRCDVYALGMSIYELIAGKLPFPSGEVADVLRKHQEEDPMPPSVFRPDLPEVLDRAI
                     LKGIARDPNLRFQTCDELMAALIAAHRQTLRKAPQRVLVADEDPLVLDQLRSCVEAAA
                     PDAEVVMVSDGEQVIQEAEKLSVGLVIMDLSLPRLNAVEVCATLRGMPATQNLPVVVL
                     STRSSRGDRALLRQFGVRAVVQKSLGLDGLTSIVRAVINERPI"
     gene            10872..10945
                     /locus_tag="PKL17_15395"
     tRNA            10872..10945
                     /locus_tag="PKL17_15395"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10906..10908,aa:Met,seq:cat)
     gene            10986..11303
                     /locus_tag="PKL17_15400"
     CDS             10986..11303
                     /locus_tag="PKL17_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46170.1"
                     /translation="MEPGRAEQLSVEHNRLRLRKRHRNLLRERIEVGLTDERFAEQHP
                     VDLERIGGHRQTRRDELKAIEAATGLPRNPHAQTDIGDGGCDEQSIAWIGLRDSIDGE
                     LGL"
     gene            11272..11787
                     /locus_tag="PKL17_15405"
     CDS             11272..11787
                     /locus_tag="PKL17_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46171.1"
                     /translation="MIPSTVSWVCNGANSHRAKRPPPGISDDPIVRSPSSDADKRPRL
                     GDDVYAARKRAPIAALAKNPVRSPLVLATLCCGRSVAKSSGTSSLLVMRNVAWGRKGR
                     SPDLASSESAPRKRVVGVPLAPRFLAMVLMVPPASGNHSGSSLPKTRCASVRVAVSVP
                     SPPYVASTSMR"
BASE COUNT         2277 a         3451 c         4032 g         2381 t
ORIGIN      
        1 gggttgccat cgtagcgacg acttctgtgg cgatgccaga aaccgtcccg cgccagccgg
       61 catgggcggc ggcgacccgt ccttcgtcat cggcaacaag cagcgacacg cagtcggcgg
      121 aaagcacgcc cagcaccagg tcgtgtgtcg tgaccattcc gtctgcttca cgccgcgcaa
      181 gctccggact gtcaagatgg agcaccttgg caccgtgaac ctgccgcact tgcgaaagcc
      241 gcgcgagatc aaaaccaccg attgtggcaa cgcgggcgaa gttgcggctt acgcagccag
      301 gttcatcgcc ccagttccgc ccgaggttta gcgaggcaaa ccgcccttgc gaaaagccac
      361 cctggcgcgt ggtgaagccg tgccggacag aaagagagga gagcagaggc gacagcagca
      421 cgctcggctg gtcattcgca gggacccaac agtcgtttgg gctgtgctgc gacatcgtct
      481 cgctcggctg tcctgtagat gccaggtgca tggccggcga ctctatcacg ggtgtaccga
      541 agcaggccga ctgccgaccg cattccacgg cagtagcgga ctatcggcgc gccctcggag
      601 actggtcagc gccgacagga agtctgccgg cggtgccgat gcaaagctga gcctttcccc
      661 cgtgatcgga tggacaaagc cgagcagccg ggcatgcagc gcctggtggg taagctgggc
      721 agagacgatc tgcacattgc gctggcgcga cgggcgaccg tacaggggat cgcccaggat
      781 cgggaaaccc tgttcgctca ggtggacgcg aatctggtgg gttcgcccgg tttccagtcg
      841 cagctcgacg agcgttgcac cgggtagtcg ttccagcact tggtagtggg tgacggcctt
      901 tttgctgccc ccgcgggatg tgaagcgctt gcgatcgcgg ggatggcggc catagcgggt
      961 cgacagggtg ccacgttcgc taatcacgcc gtcgacaagg gcgacataac gtcgctcgat
     1021 cgagtgggct tgaaactgaa cttgcaaacc gaccagcgtc ggttcgtctt tggcgcagac
     1081 cagcaccccc gaagtcccgg aatcgagtcg gtgaacaatg ccgggacgcc gttcaccacc
     1141 gatcgaaagt ggcgcgtgct ggatggccgt ggctgcgccc gctcccgctt ttcggtccgt
     1201 gctgggttcg tccgtctcgg catcgacttc gtcttcgtcg ccgtcttcat ccgcaagcgg
     1261 tcgcccaagt ggtttgggca gtgatccgca gtggtgaagc agggcgttga cgagtgttcc
     1321 agtctcgtga ccgggtgcgg ggtggacgac catcccggct ggcttgtcga tgacgatcaa
     1381 gtgacggtct tcgtaaagta ccgtaagggg gatgtcctcg ggctcatcgt gggtcggggc
     1441 cggcggtgag gggacaaaac gcaaggtctg cccggatcgc agcttttgcc caggacggca
     1501 ggctcgacca tcgagagtca cctctccggc ttcgatgcgc cgcgcgagct gagaacgcga
     1561 aaacggcagc tcctgctgcg ccaaatagac atcgaggcgc aggccagcag cggacggcgg
     1621 aactgtaaaa acgatcggat cttgggattc ggtcaccaga tccgcgactt tacatgtcct
     1681 ttggacttga gcatcacatc aacgatcgcc cggtcgaaca gtcccagttc atagatatcg
     1741 ggcgtcacac ggctcttgaa cagcgtgcgg ccttcttcga tgtgatcgtg catcacgttg
     1801 aagagggtgt cgttttcgat gccttcgatg atctctttct cgtgatagag ggtgaggtca
     1861 gacgaaatgg ctcgcgccag ctggcgagca cgctcgggtt tgtcgattag cgccatagtt
     1921 tgcaatgtta acgaggcgta gagaaaagct caacggcgtt cgtttgcttg ggcccacctg
     1981 atggtctgcg agtgaggcca tcgcgacgca aagtttgccc aagcgaaaaa gagccgctac
     2041 cttgtgcgcc cgcccaagtg ggccaaaacc tctgccatga cgagcctgca agagatccct
     2101 gtttccctcg atgcggagac ccagaagcgc tacctaagct acgctctgtc ggtcatcacg
     2161 tcccgtgctc tgcccgatgt ccgcgacgga ctcaagccag ttcagcggcg catcctgtac
     2221 gcgatgtatc acgaccaacg gcttctgccc gacgccaagt ttcgcaagtg tgcggcggtc
     2281 gtcggcgatg tgctcggtaa gtatcacccg catggcgatg cctcggtgta cgaagccctg
     2341 gtccggatgg cgcaggactt ttcgctgcgc tatcccctgg tcgacggcca cggcaacttt
     2401 ggatcgcagg atggcgacgc accagcagcg tatcggtaca ccgagtgccg gctgcgtgcg
     2461 acggcactgc tgctgctaaa agagctggga cagagcacga tcgactggcg accgacgttc
     2521 gacggtgtgc gcagcgagcc gatcgttctg ccttcgcagc tgccgaacct gctggtcaac
     2581 ggcgtgcagg gcattgccgt cggtatggcg acgtctatcc caccgcacaa cgcgggtgag
     2641 gtcatcgacg cttgccttgc gcttatcgac gaaccggagc tcgatagcaa gtcgctgctg
     2701 cgctacgtgc gcggaccgga cttcccaacc gctggcgagc ttttgacgag caagcaagag
     2761 ctgcaagagg tctatgagac cgggcacgga acgctgaagc tacgggccac ctatcacgtc
     2821 gaggagccag ccgaccggcg cgaaggtcgg cgcatcatcg tcaatgcgat cccgtatggg
     2881 gtggtgcgct cggcgatcat ggaaaagatc ggcgagctgt tcgaggaaaa gaagctgccg
     2941 atggtggtgg actgccggga tgagtcgacc accgacgtgc ggatcgtgat tgagctaaag
     3001 cgcggcgccg acccgcagct tgtcatggcg tttctgttta agaacacagc gctcgccacc
     3061 accgtgcagt tcaacatgac ctgcctggtg ccgacggaaa atcccgagat tgggacgccc
     3121 aaacagctcg gcctgcgcga gatgttgcgt cactttttgg acttccgctt ccaagtggtg
     3181 cggcgtcgct tgtcgcatca gcttgcggag ctgaacaagc gcatccatgt gctcgacggc
     3241 tttgccacgg tctacgatgc gctcgacgaa atccttgcga tgattcgtcg cagcgagggg
     3301 aaggccgacg ccaaagagaa gctgataagt cgcttcaagc tcgatgacga tcaggccgaa
     3361 gccatccttg agctgaagct gtatcgactg gccaagctgg agatcctggt cattcaggaa
     3421 gagctttcgc aaaagcgcaa agagcgggcg cagctggagt cgctgctcca gtcggatggc
     3481 aagcgctggg aacttgttcg gcgtgagctt gggacgcttc gtgatgagct ggcggacaag
     3541 cggcggacgc ggattggcgt tcccgccgag gaaccggctt tcgatgaaac ggcctttatc
     3601 gcgcacgagg actgcctggt ggtgctgacg cgcgatggct gggtcaagcg ggtagggcag
     3661 cttcgggacg tcagctcgac gcgagtgcgc gagggcgacg aagtgcaggt ggtcctgggc
     3721 ggctcgacca aagagctggc ggtgttcttt tcgagtcgag gttcggcgta tgtttgccgc
     3781 atcctcgaca tccccgcgag cagtggttac ggcgagccgc tgaccaagca gtttaagttc
     3841 gacgatggcg agacggtggt ggcagcgctg tcgcttgatc cgcgggtgcg cccggcggcg
     3901 gacacccatt tggttgccgt gacccgaggc ggcctggcgg tgcgatttgc cttggatgcg
     3961 cacgccgagg tgtcgacgcg caacggtcgc ttgtttgcca aagtttcaga cggggatggc
     4021 gtgctggggg tgcgggtggc gcgcggcgat gcgctgctga cggtagcgac gtcgcttggc
     4081 cgttcgctga cctgccgggt cgatgagatc ccggtgttac agggtccagg caagggcgtt
     4141 cagatgctga agctcggcga aggcgaccgc atgatcggtt ttgcagtcgg tgagacgctc
     4201 aaagtcgaga ccgacaaagg cgcgacgcac gaactgggtg gatctgccgc agatgtgacg
     4261 gcgcgcggcg gcaaggggcg tgaggtgtta aagaaaggca agcttgtgcg ctgcctgttt
     4321 gctccgccga cggtgccgca gctaacagcg aaagtggtga gtgcgcaggc tgcgcaaggt
     4381 gatgctccgt cggaagcgac cccgatacca gacatcattg ggccgctgtt ccgacgtccg
     4441 agctagggca gggggcgtcg gggaaggtgt gggtcgagtc ggactacagg acgacgttca
     4501 tcatcgcgcg atacggcgcg gtggtgaagt cgcggacgcg gcggtcgttg ccacggctga
     4561 cagcgtagag catggctccg agcgcgcccg gcgtgaccat gctgtccggg aactgcgccg
     4621 gggtgtacag cttgccgctg tccggatcgt agttcatctt gccggtgcgc gagacctgac
     4681 cgaaccagcc cggcttcatg tagccgttcg acatgacgta catccagttc gacgcacctg
     4741 gggtcgagtc ggtccagtcg gctgccttgt tgctgaacgg acctttgggc aggtcgcccg
     4801 agacggtcat gaccaggttg tcggacagct ttttcgacgg gtcgaccggg tcgggaatgc
     4861 cgtttaggta gtccatgaag gactggaaca ccagcgcaag accgtccgag atgcgggtga
     4921 cacgggcctg gtcgctccac acaaagtgcg ggtcgtcgtt gaatgctggc atcatgacct
     4981 gcgagcacag acccttcttg aaggcattgg ccgcaacgat gaggttgttg gcaatggcct
     5041 tcacgttggc atcaatgctt gccgggccgc accatgcgtc tgcctgacca gccaccgggg
     5101 cgagcacgct gccgaggttc tgtgacacaa gcgagatggc gacgcgcgag tcctcgtatg
     5161 cgcgctgata ggtggcgcgg tcgacggtct tcaccaggcc gaggaatgct ttgtaatact
     5221 cctcgtagag cttggcgttg ttcggatccg acagacgcgc tgccagcttc gacgccgagc
     5281 tggagaacag gttgatcatc gacatgtagt caggcaccga cgctggcgaa ggcgaaccgg
     5341 tggccgttcc gtatggcatc gtgaccgcat ctttgttgat gccgatgacc ggaaccagcg
     5401 actggaccgt ggtctggatc gacgcgcagg ccgcaaacga tccgatggtg cccgtcggaa
     5461 tggtgttgct attgccggcg ttcttcggcg ccacttcgtg accggtgttg gtgccgcaga
     5521 gcatcgcggt cacgagcttc ttgtcaccgt agctcgacca cagcggctgg ccgttgacct
     5581 tgcggtgata cagcgtccga tcctcgagcg cgcccgaggt gatcttgccg gccttggcca
     5641 tgtcgtccca gggaacactc gggttggtct ggaactcggt gatggtgccg ggaaccggcc
     5701 aaaccatcga gaaccaagcc aagccaccag tgccaccgag gatgtggacg gtgcggtttg
     5761 cattgatggc accagcagcg agcgcgctac cgccaatgtc atcgagaagc tcgagcgcgc
     5821 gggttgggcc aaggcccagt gcagcgctgg cagccataac acctttgatg aagtctcggc
     5881 gggaggctcc ccgcaacgga aaatcgcgtt tcatgtagtt cctccctcct taatcacaaa
     5941 gataggcaga cgaagcgatg gttgcgacgg tcattcgctt ggcgcgcatg atgtcgttgc
     6001 tggtgagcga tgcgatccgc ttgacgccgt tgctgcactc ggtgagctgc accgtggtaa
     6061 gcggtgagcc aaggaggcac gcaaagccat cagcgttgca ggtgttgtcg gcattgaaca
     6121 gcgtggcgcc ggggcaagcg gtgccagtgc cgatgcgatc cgtcgttgcc gtcgggatca
     6181 gctcctctgc aactgccagc atcatgtcga acatgcgggt caggccgccg gtcgagttgc
     6241 gatccgcctc tgcgacgcgg gccgggtagt tggcaacgcc catgatgttg gcgccgccct
     6301 ggtacagaac acccgccgag tcagccgcgg ggtttgcgat gttgatacca cgggtggtga
     6361 ggatggtgcc gagcgcctga tacatgatct tgccgcagac gtgcatccgc tggccatcag
     6421 acggcagcac ctggcgtcca tccgcaaacg ggttgccgat gttgcccagg atgtcgtcgt
     6481 gatcgaacga cttggtgctg gtgtttggcg aaaccggcgg ctgcgtgacg tcggtcggct
     6541 gcgttggatc catggtcgga tcgggggtag acgccgcatt ttggcatcca atgaggccca
     6601 gcatcgagac caggcccaga cggacgaaga aaccagactt gcgcttcatg ttgcttctcc
     6661 ctttcgttct ggtcctagta gcgaatgaag tacgcgctct tgtagacgcc acggatgaac
     6721 tctttgaggt tccacttgtt gttgtcgatc tgctgcgtaa gctgcgcctc gaagtccggg
     6781 gtcaggatcg cttgatcgac aacgacgtca ccacgcgaca tggcccagtt ccagacgcgg
     6841 gtggccatgc aggccttgaa tgccgggtca gcagccatcg ccgcaccgaa cgacttgatg
     6901 tcgggcgtcg gctgaccgaa ccgataggac agcggttcac cagctgcgag ccagtcgctc
     6961 agcttgctgg tcgggttgcc ggcgaccggg gtgacgacct ggatggtcgc cttcatgatg
     7021 ccgtcggctc cgaagttggc gaacagcggc gactggtgat tgatcgtgct gtggcagttg
     7081 gcgcagatga gcgagctggt gtcctggaag tcgaccagcg ctttcggatc ggtgccaaga
     7141 ccggtcacgg tcttgatgtc ccacggcgag ttgtagacgc cgcccgcgaa ctgaaccttg
     7201 gggccgttgg tttcagccgg gaagcgcgag cacagcaggg cttcttgaaa ccagcggacg
     7261 cggcggaagg ccattgcgga atagaactgc gcctgcacac cctggtcggt gagaatgccc
     7321 accgccgccg ggttggtgca ggtgctctgc gtgaagctgt cggtgccggc gttgtacgtc
     7381 tggcaagtgc cggtgtccgc cgtcagcaga ttggtaaacg gctgaccctt ggcaaccaga
     7441 aacgtcgcgt aggtcggcgc gaagtcgagg ttgacgttga ccacacccgt gggcagcgtg
     7501 acagggagca tgccgcccat cttgaaggtg tctttccaga atttcaggat ctgctgattg
     7561 aaggtcgggc gactgatata cgtatcaatg atctgactgt aggccgtcgt ctgatcggtc
     7621 gcagtttgta gctgcttgat ttctgccagc gttgggtagt ttcccgtcag cttgagtgct
     7681 gccgagcgga gcgcaagatt gtagtccagt ttgccgtctt gcagggccag cttgtcggtg
     7741 tccgcgacgc tcggtggcag cgtggcgcgg aaaccgccgg ttgggtccgc tgcggtatca
     7801 gtggacgttg cgccgccgct gcacgcggga agggcggcca agagcagagg cacaatgtgt
     7861 ttcttcatgg agctcactgt catgatccct ctcgattctt tcggtccgat ccgttgtctg
     7921 ctggggggca cggcgggagt tctgccgttc ccctcgctcc gaaccccgaa tcagagcagg
     7981 ttagggttac tttcaagcac ctacttctct gccgcggccc acgcagacac cgcggcctta
     8041 aagctggtaa acgttgcatc atccagcttg aagacgtgac tggcgatgcc tccttgcgtc
     8101 ggaggttgca cgtactgcac cagaatgctc ttgtcgggcg tggtgacgtt gatgcgtccg
     8161 agcgcgtcga cacagagact tgccagtgcg gtttgatctg tcttcagtgc tgcgtccatc
     8221 gggaaggcgc gcgtcgcacg gctggcttct gcgcgggttc cgacggcgct gtgacactgg
     8281 ctggcacaga tcgcgagcgt attggtgacc ggcggtgtaa agagattcag actcttacac
     8341 gcgacgttca cgggcatcgg gttgacgacg tttatctgct cgaagataaa cgcgacaagc
     8401 gagctggatg ccggaacgtt gggcagcagg atggcacccg aaccgatcgt gacggtcttg
     8461 cccgcatctg cggttacgtc gaccgtcgag agcgcgtcgc tcggctcaag agtcgcccca
     8521 ccggcagtaa tataaacgag gcgcgggtgc ttgatcttga tgccgccgcc ggggcctgcg
     8581 gtcagttgca ggttggtcag acggaacgtg cggcccgaga tgagctgcat cgaaaacgtg
     8641 atgcgcgcga gtggatcttg ggtcagggca cgcaggctga tgtagtagtc accgacgata
     8701 aaaggcacag caggagtggt tgggctgttg cctgacgcgc cgcgctcggt ggcttcgacg
     8761 tcgagccaac ccttgacgat gttggtctga tcgacggtca gcgccggtcc tgcgtgggca
     8821 cctttggtca aaagcgggct ggtcgaagct tcggacggga caaacagtcc gccccgatag
     8881 cccgtgatcg atgcgtattc cgatcctgtc gccaaaaagg gcgaaaatcc gtttacgccc
     8941 gccgagtgac aggaacagct ggcaacgagc tccgacttga cgcggtattc gaaaagacca
     9001 cggccatcga gcacttcgtc ggaggtgttg ttcgagttgc cggtgtcttt ggtgatgatg
     9061 ggaggggcac agccgagggc taggccaagc gcacccagca tcgccaggcg cggcagaccg
     9121 aggttatcgc ttggtaatgt tctcgacggg aatactcgtg tcattgggtc gctcccagga
     9181 gtcgtgcgca ggccaatctg ctcgtctgtg gttggctgca ttggcatgtc acgacaaatt
     9241 ttactgatca attttcggtt agaaaaaata caagatgcga tctcattcta aaacgatgaa
     9301 aaagaataat caccgtcgaa gattgattga gatgccgctg atcgcatcgt ctgatgtcat
     9361 ggatgcgtaa ttaaaaggta attttcatta acatgttttt gttgtgcata cagggcaagc
     9421 ttggctagac tgttggggcc cgtcgctgat gcgacttgcc tagcagcgcc ctcggagacc
     9481 atgccgccct cgaatcccaa gcagccgccg gccaagacgg cagaaggtgc cgcaggcaaa
     9541 gcccaggatc gcgcgccacg cattggcgag gtgctcgccg ataactaccg cctggtccgt
     9601 gaaattggcc gcggtgcgat gggcgcggtc tatttggccg aagacctggc gctagagcgt
     9661 caggtggcgg tcaaagtgct gctgcccgag cacgcagtgc atcccggtca agctgcgttg
     9721 tttcgtcggg aagcgcggct tttggcggct gtccggcatg aaaacgtcgt ccatgtgcac
     9781 acgctcggtg agcaccgcag catcccgtac atggtgatgg agtacatcgt cggcccgacg
     9841 ctggcgtact accttgtgca ggcggtgcgg gtgcacgatg cgatcccgct cgatctggcg
     9901 atcagcctgg cgctgcagct ctgtcgggcg gtgcaggtgg ttcacgccgc tggaattatc
     9961 caccgagaca ttaagcctgc gaacgtgatg gtggcgccgg ggccgcgggc gatcctgatg
    10021 gactttggac tggccaagag caccgactcg cgcggttcag ggacgcacat catcggtacg
    10081 ccggattatt tggcgccgga gcagataagg cagcgtccgc gtacacccga agaagcccgt
    10141 cgctgtgacg tgtacgcgct gggcatgagc atttacgagc tgatcgccgg gaaactgccg
    10201 tttccaagcg gtgaagtggc cgatgtgctg cgcaaacacc aagaagagga tccgatgcct
    10261 ccgtcggtgt ttcggccaga cctgccggaa gtactggatc gggcgattct caaaggcatc
    10321 gcgcgcgatc cgaaccttcg gtttcagact tgcgacgagc tgatggcggc gctcattgcg
    10381 gcgcaccggc agacgttgcg caaagcgccg cagcgggtgc tcgtggccga tgaagatccg
    10441 ctggtgctcg atcagctgcg cagctgtgtc gaagcggcgg cgcccgatgc cgaagtcgtg
    10501 atggtcagcg acggtgaaca agtcattcag gaagccgaaa agctgtcggt tggactggtg
    10561 atcatggatc tgtcgctgcc gcggctcaac gcggttgagg tctgcgcgac gctacgcggg
    10621 atgccagcga cgcagaatct gccggtggtg gtgctgtcta cgcgatcgag ccgtggcgat
    10681 cgggcactct tgcgacagtt tggcgtgcgc gcggtggtgc aaaagagcct ggggctcgac
    10741 ggtctgacct cgattgttcg cgcagtcatc aacgagcgac cgatctaagg cgaaaaagcg
    10801 cagcttcgcg attgacagaa gcgctggggc tttgatagaa gctttgtcac gtctttttga
    10861 gacggacttt tggcacctta gctcaactgg ttagagcagg cgcttcatac gcgcaaggtt
    10921 cggggttcga ttccctgagg tgccacagcg ggggacagga tgcggagcgt cggtcccatc
    10981 gaaagatgga gccgggccgc gcagaacagc tctccgtcga gcacaatcgg ctcaggctgc
    11041 ggaaacgtca tcgtaacctg ctgcgcgagc gcatcgaggt ggggctgacc gacgagcggt
    11101 ttgccgagca gcatccggtg gatctggagc gcattggcgg tcaccggcag actcgacgcg
    11161 atgagctgaa agcgatcgag gctgcgaccg gcttgccacg gaacccgcat gcccagaccg
    11221 acatcgggga tggtggatgc gatgagcagt cgatagcttg gatcgggctg cgtgattcca
    11281 tcgacggtga gctgggtttg taacggcgca aacagccacc gcgcaaagcg accgccaccg
    11341 ggaatcagcg atgatccaat cgtccgcagc cccagcagcg acgccgacaa aagaccccga
    11401 ttgggtgacg acgtgtatgc cgcgagaaag cgtgctccca tcgcggcgct ggccaaaaat
    11461 ccggttcgct cgccgctcgt cttggcgacg ctttgctgtg gtcgcagcgt cgcaaaatcg
    11521 agcggtactt cgtcgctgct cgtgatgcgc aacgtcgctt ggggtcggaa gggcaggtct
    11581 cccgacttgg ccagcagcga aagcgcacca cgtaagcgcg tcgtcggtgt tccgctggct
    11641 cctaggtttt tggcgatggt gttgatggtg ccaccggcga gcggcaacca cagcggtagc
    11701 tcgctgccaa agacgcgctg tgcttccgtc agggtcgccg tcagtgttcc gtcgccgcca
    11761 tacgtagcga gcacgtcgat gcgctgatcg gcaaagcgac caatcgcttc ccgcagcgcc
    11821 gtcggatgtt ccgtcacagt gagctggcag tggagtgcag gaaattcgcg acagatcgct
    11881 tccagcccgg cgaccagcga cggatcttgg gcgcgtcggg catgcggatt gacgatgatt
    11941 ccgatgcgca cggcccagtc ctttcagttt cccgcgagca gcgttcgcag atactgaccc
    12001 gtgaagctag atgcagactc ggcgacctgt tcgggcggac cttcggcgat gataaggccg
    12061 ccgcgctcgc caccctcggg tcccagatcg atcacccagt cggcgcactt gatgacatcg
    12121 agattgtgct cgatcaccag c
//
LOCUS       DAORGN010000152        12122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2192_length_12122_cov_5.763404, whole genome shotgun sequence.
ACCESSION   DAORGN010000152 DAORGN010000000
VERSION     DAORGN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2192_length_12122_cov_5.763404"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKL17_15410"
     CDS             complement(<1..439)
                     /locus_tag="PKL17_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46172.1"
                     /translation="MTNKRPLTTELLAALTAAKVADAELADLPLIEEYRLVRGLGHGA
                     MGCVYLAQDTLLDRKVALKFLRSLRHDEAARQRFFIEARAIARLSHHNVVGIYRISEV
                     LGQPFLVSEFVAGRSLDKLPLPLSAAEVRTLGIGLSHGLSAAHR"
     gene            571..1206
                     /locus_tag="PKL17_15415"
     CDS             571..1206
                     /locus_tag="PKL17_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46173.1"
                     /translation="MRRLGMREIALCCGLLWGAVAVIPSNASATVMTKLSLADMTARA
                     EHVLLGRVEQIRSRMLPGHDGLIVTEVQIRCTRSIRGQKEGALVQVRHLGGSVGELGQ
                     KVFGEASYQVGEEVVLFLEPREGALYAVGMAQGKMHVDRSNGNARVSVDLGGAELIAP
                     TGNDALPTAQGIALDALLGQLAELAQRIPAPSARIKSATGGSDGAKAGQKP"
     gene            1203..2162
                     /locus_tag="PKL17_15420"
     CDS             1203..2162
                     /locus_tag="PKL17_15420"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNK46174.1"
                     /translation="MNKGRFLSVVAAALALSIVPSASAYVRSRTQDSCLPIFWRQSCV
                     YIQPDSGFVSDMAGADVERIIQKSIGNWMTRASGSFVKLNYRAADGHKDVTYKDHLSV
                     VVFRTGSHFCRPATDGNSQVCYDASAAAVTTVSYINKKGDPDDGLIVDADIELNAVNN
                     QFVEIGGNLPAADSRNQTDLENTLTHELGHLLGLDHTCRLSTDPACLADDKGQPRQTC
                     DTIDKGRFLNPTYDTIYKTSMFAIAPPKDIEKRTPKSDDVAGIVAVSPLVSDPKVCVD
                     PAAMASSGCSCEVGMAKKTPPALWFAVLGGLAGVLMLRRKKSI"
     gene            complement(2159..3349)
                     /locus_tag="PKL17_15425"
     CDS             complement(2159..3349)
                     /locus_tag="PKL17_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46175.1"
                     /translation="MPVSFGVVTLCAWMAVVGLAVLVRGRPFGTFAGIIVGLHSLIAA
                     ELAPMFQPIWPLFVALHITVYLNFLLLLRPALRPWPVRVLINWPGSFFWGGTLLAMPW
                     AVVRALGFTPWGVAIPYGLAVIGFLQSHATFPEELDLVVGDGDSAHPMPRPHRRGTFR
                     QDRPLRIVQITDPHLGPFMSVGKLRRIAEDAVKRNPDLVLLTGDFLTMESQRSPEPLR
                     RALEPLKALRGRVFACHGNHDHEAPQLVRQALEANEIHLLVDEAAIVDTEAGRVHILG
                     MDFAWDRRAERMQKVCDAHPRIPNTTRIVLLHDPGAFKHLPEGQADLVLSGHTHGGQV
                     GLLSLGLPWTFLRLFGIRIPDHGFWARGRDRMYVHRGTGHYGFPLRVGVPAEESLLRV
                     HRAA"
     gene            3450..4850
                     /locus_tag="PKL17_15430"
     CDS             3450..4850
                     /locus_tag="PKL17_15430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK46176.1"
                     /translation="MSAHVLVADDEALILQSLRGVLLQEGFEVTAASSGEQAWQTFVA
                     TRPDVVLLDLVLGDITGIDVLRRIRQLSPETKVILISAHATLEQAVEAMKLGGYDFIR
                     KPFELEEIVTTVRNAARTSELEGRVDYLARLTRQQADSGGLLYASTQMANKLREIDVI
                     ARSPVQTVLIRGESGTGKQLVAQLLHERSTRRSGPFVELNCSTLPETLVESELFGHER
                     GAFSDARERKLGLVEIADGGTLFLDEIGDLSLSAQAKLLTFLEQRTFRRVGATALRKV
                     DVRVIAATNRDLKAMAQGKEFRGDLLFRLNAITVELPPLRERPDDIGLLAEHFLHQAS
                     QEYHKNWQRLSTETKDLLTSYGWPGNVRELKAVISRAALLYDEPELRPVHLPVELVQS
                     GPPVRRETTQPAELSPDRPAGAGPDGPIPTLAEIELAHIRRVLELCGGNRTVAAQHLG
                     VTRQTLSRKLEEGAVS"
     gene            complement(4875..5504)
                     /gene="can"
                     /locus_tag="PKL17_15435"
     CDS             complement(4875..5504)
                     /gene="can"
                     /locus_tag="PKL17_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNK46177.1"
                     /translation="MSSLQHLLVQNRRWAEEMVERDPDCFSRLAEQQTPNYLWIGCAD
                     SRVPANQITGLAPGEVFVHRNVANLVIHTDLNMLSVLQYAVDVLEVRDIIVCGHYGCG
                     GVRAALEGKQIGLIENWLRHIQDTARHHKIELASMPTDDARAARLCELNVIEQVLNVA
                     ETTVLEAAWERGQRVSVHGWIYDIHDGRLRDLDIELHSAEDLARRRALE"
     gene            complement(5607..7214)
                     /locus_tag="PKL17_15440"
     CDS             complement(5607..7214)
                     /locus_tag="PKL17_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNK46178.1"
                     /translation="MSRTAVYKPDLKADLVSGFLVFLIALPLCLGIAMASNFPPIAGI
                     VTAVVGGIIATFVGSAELTIKGPAAGLIVIALGAVQELGNGDPVAGYHRALAVIVVAG
                     VIQVVLARLRAGVLGDAFPAAVVHGMLAAIGIIIVSKQAHTVLGVAPTAREPLHLLAE
                     VPHSLMTMNPEVALIGGLSLLLLFGIPKLPFPWAKKVPAPMVVLLIAIPLGMFLDLGH
                     EHTYTFHLHEYKVGPKYLVQLPGSLAKAYSPPDFSYLLNWDSIKYIVMFTLVGSLESL
                     LSAKAVDSLDEERRHCDLNRDLQATGVGNVIAGMLGGLPMISEIVRSSANINSGAKSR
                     YANFFHGLFLLGMVALLPGLLQRIPLAALGAMLIYTGVRLASPSEFVKVFRIGPEQLL
                     LFVTTLVTTLATDLLVGVGAGVLLKLVLHLRHGAPLRGLFSTRIEEEVIDESTLRFKV
                     YDAAIFSNYLSLKKRIDAVPATIKTLQLDLEGTRLVDHTVIEKLHHMAEDWQRQGRSL
                     DLLGLDEHEPLANHQLSARRKTVSATA"
     gene            complement(7414..8013)
                     /locus_tag="PKL17_15445"
     CDS             complement(7414..8013)
                     /locus_tag="PKL17_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46179.1"
                     /translation="MPSQIELAQKCSAQFEAKGTVWNGRVKALSRHCSLSEVRALLSD
                     PARKLLDEPPPASNWIAGSALVELDAAIYDLRGSDVLARCSRETSETAGTTVLRTMAE
                     GMLRLFGTSPATLFERMGQVAATSVRGVDYQYTATGPKSGVMTICYRHSANIPYCVFV
                     AVAGGMGAIFAFLHVKGQVSAPEVSTQAPRNSATITVRW"
     gene            8215..9183
                     /locus_tag="PKL17_15450"
     CDS             8215..9183
                     /locus_tag="PKL17_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46180.1"
                     /translation="MRFDHLLFAASLAVCSSLAGCESPPKGGPDMSGGGSVVSPEQAC
                     PALIKAYCAKATECMPFNQALGFPDAATCEARLNLTCPQLAKAMGTSVTGDDLQACSA
                     KFSSISCYDYLSLSNPVQVCDFKPGKLTDGSACGEGVQCQSLYCKKDSTMDCGKCTAL
                     GKSGSVCVTNSDCDKGLACVGAAGAKQCTAYLTMGASCSTAANSPSCHPTLACRNGVC
                     ATPAKLGESCSATTQDCDKLAGLTCPLALKCVGYATAQLGGACGQQGSTYILCAGGSW
                     CDSTSKKCMAPAPDGMACSDATGPKCQAPANCRAGICKIADSATCM"
     gene            9240..9851
                     /locus_tag="PKL17_15455"
     CDS             9240..9851
                     /locus_tag="PKL17_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46181.1"
                     /translation="MKRSLSRWLLPLSFLLGCGGTPASPPQWYYTCGDPVCRGYTGTA
                     GVAKCTTEKAGASCTMEGVECDPQDSCNRKLRCATSDPTQQTGGCPISRRSAKTDIQY
                     LGDTELHKYADEVMQMKLATFKYRSGGPVRLGFMIDDNPQSKSVDPERDMVDLYGYTS
                     MTVAALKVQQQQVSDLQKRLDDLEKQLKSCRSAQPAVSVHKRK"
     gene            9933..10412
                     /locus_tag="PKL17_15460"
     CDS             9933..10412
                     /locus_tag="PKL17_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46182.1"
                     /translation="MQMLPVSRRVLLPTLVFGVALLTQRGALADLAPRPRVSIAVLDS
                     AHCPMPALKNAVEQVEAELESCAKEERARGKTVLHCSFDKSGHVKSCSVASRSGHFSK
                     AQNECFRKALVQVQIPLAKWDGGSKPSLCTAQVQVESVIPPYRRPRPHGSGDIIPDF"
     gene            complement(10413..>12122)
                     /locus_tag="PKL17_15465"
     CDS             complement(10413..>12122)
                     /locus_tag="PKL17_15465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46183.1"
                     /translation="KPENIMIMNRDGDPDFVKVLDFGLAKIRIEALVEDVSQRSETLT
                     KYGTIFGTPAYMAPEQAAGGEVDGRTDLYALGVVMYELLTGWVPFDANDPAVILRQQI
                     LAPVPPMAQKAPSVKVPATLEAMVMKLLEKNPDNRHKDARALLDALTELAMAEGLRYE
                     PSTPLGRLNIPNSGIRDIDSRPTVMGDKDYPAQAAATGGESVAQIEIVSPSAVQIPAV
                     AGAAASAKATGGVPATKAGDSGGKHPALPAGKDSARDKDEANRAVGHAATVPALSASS
                     NEIVPAGSAGGLPAKLALVLSTISQKWSALLGFVRGKLPEKHRGVSQWALGTAVAAAL
                     LLPTFFLFGLLIGSDEDSDSAVVGMVGFASDADMQKAVEKGPAALLKLRNKYPNDSRT
                     HRALVKAYGKAGELGSALQAIPPLLTVDPAAAYDDQILRLVADAALQPQTSEPAVQLL
                     EQKMGEYGVDMLIELADRTTMEPYRGRLTMSLSKEAVRKLASPDGVMVLDLRNAASCE
                     AKKALVAKAGKQGGKRTLETLLKLQIPTGCGPGAQTDCWPCLRKGNALQSAINEIRQR
                     GIL"
BASE COUNT         2477 a         3517 c         3719 g         2409 t
ORIGIN      
        1 gccggtgcgc tgcggaaaga ccgtgcgata gtccaatccc gagcgtgcgg acctccgcgg
       61 cagacagcgg cagcggcagc ttgtcgagac tgcgccccgc gacaaactcc gacaccagaa
      121 acggctgacc cagcacttca cttatgcggt aaatcccgac gacgttgtgg tgtgacagcc
      181 gggcaatcgc gcgcgcttcg atgaaaaaac gctgacgtgc tgcttcgtcg tgacgcagcg
      241 atcgcagaaa cttcagcgcg actttgcgat caagcaacgt gtcttgcgcc agatacacgc
      301 agcccatggc gccgtgtcca agcccgcgca caagccggta ttcctcgatg agcggcaagt
      361 cggcaagctc tgcatcggcc acctttgcgg cagtcagagc ggccaggagt tcggtcgtaa
      421 gggggcgttt gttcgtcacg cagcacgcca catgcagtat ctgtcaaagt agcttgccga
      481 aaccccgtcg aagatcaagc tttgctcgcc aattttgctt gcctaggcat gatgaagata
      541 aagacccgaa agggaaaagg agtccttggg atgaggcgct tgggaatgcg tgagatcgcg
      601 ctctgttgtg gtcttctgtg gggggcggtg gctgtgattc catcaaacgc ttcggcaacg
      661 gtgatgacca agctgtcgct tgcagacatg accgcgcggg cagaacatgt gctgctgggt
      721 cgcgtcgagc agatacgctc tcgaatgctg ccaggacacg atggcttgat cgttaccgaa
      781 gtgcagattc gctgtacgcg cagcattcgc ggccaaaaag aaggcgcact tgtgcaagtc
      841 cgtcacttgg gtggttccgt cggtgagctt gggcaaaaag tgttcggcga agcgtcctat
      901 caagtcggtg aagaagtggt tctgtttttg gaaccccgtg aaggcgcgct gtatgcagtg
      961 ggaatggcgc agggaaaaat gcacgttgat cgatcgaacg gaaacgcgcg cgtgtctgtg
     1021 gatctcggtg gtgcagagct gatcgcaccg acgggaaatg acgcgcttcc gactgcgcag
     1081 ggaattgcgc tggatgcact cttgggtcag cttgcagagc ttgcgcagcg gattccggct
     1141 ccatctgcgc ggatcaagtc ggcgacaggt ggaagcgacg gtgcgaaagc ggggcagaaa
     1201 ccatgaacaa aggacgattc ctatccgtgg tcgcagcggc acttgcgctg tcgatcgtgc
     1261 cgtcggcatc ggcctatgtt cgttcgcgaa cccaagacag ttgtctgccg attttttggc
     1321 ggcaaagctg tgtctacatt cagcccgatt ccggcttcgt ttcggacatg gcgggcgctg
     1381 atgtcgaacg catcatccag aagtcgatcg gaaactggat gacgcgcgct tcgggatcgt
     1441 ttgtgaagct gaattaccgc gctgccgatg gtcacaaaga tgtgacgtac aaagaccatc
     1501 tttcggtggt tgtgtttcgt accggaagtc atttctgtcg gccagctacc gacggaaaca
     1561 gccaagtctg ttacgacgct tcggctgcgg ctgtgaccac ggtttcatac atcaacaaaa
     1621 agggcgatcc cgatgatggt ctgattgtgg atgcagacat cgagctcaac gcagtcaaca
     1681 accagtttgt cgaaatcggc ggaaatcttc ctgctgcgga cagtcgcaac cagaccgatt
     1741 tggaaaacac gctgacccat gaattgggac atctgctcgg acttgatcat acgtgccggt
     1801 tgtcgaccga tcctgcgtgt cttgccgacg acaaaggcca gccgagacag acctgtgaca
     1861 cgatcgataa ggggcgattc ctaaacccaa cgtatgacac gatttacaag acttcgatgt
     1921 ttgcgattgc accgcccaaa gacatcgaaa agcggactcc caaatcggac gatgtcgcgg
     1981 gcattgttgc ggtgtcaccg ctggtgagcg atcccaaagt ctgtgtcgat ccggctgcga
     2041 tggcttcttc gggctgttcc tgcgaagtcg gaatggccaa aaagaccccc cctgcgctgt
     2101 ggtttgcagt gctgggcgga ctggccggtg tgctgatgct tcgacgaaaa aaatcgatct
     2161 aagcggcgcg gtgaacgcgg agcagacttt cttccgcagg cactcctaca cggagcggga
     2221 atccatagtg tccggtgccg cggtgaacgt acattctgtc gcgaccgcgc gcccaaaatc
     2281 cgtgatcagg aatccgaatc ccaaacagtc gaagaaacgt ccacgggagt cccaagctca
     2341 gcagtccgac ttgtccgccg tgcgtatgtc cagacagcac caagtcagct tgcccttcgg
     2401 gaagatgctt gaatgcaccg ggatcgtgca aaagcacaat gcgcgtggtg ttgggaatcc
     2461 gtgggtgtgc atcacagact ttctgcatgc gttcggcccg acgatcccac gcgaagtcca
     2521 ttcccagaat gtgaacgcga cccgcttcgg tatctacaat cgctgcttca tccacgagca
     2581 gatgaatttc gtttgcttcc agcgcctgcc gcacaagctg gggcgcttca tgatcgtgat
     2641 tcccatggca ggcaaagacc cgtccgcgca gcgctttgag cggctccagc gcccgtcgca
     2701 gcggttccgg actgcgctgc gattccatcg tcaaaaagtc tccggttagg agcaccaagt
     2761 ccggattccg tttcaccgca tcttcggcaa tccgtcgcag cttgccgacc gacataaacg
     2821 gaccgaggtg gggatcggtg atctggacaa ttcgcagcgg acggtcttgt cgaaacgtgc
     2881 cgcggcggtg tggtcgtggc atcgggtgcg ctgaatctcc gtctccgacc accagatcca
     2941 gttcttcagg aaatgtggcg tgtgactgca aaaaaccgat caccgcaagt ccgtagggaa
     3001 tcgccacgcc ccacggtgta aacccgagcg cacgaaccac ggcccacggc attgcgagca
     3061 gcgttccgcc ccaaaaaaac gaacccggcc agttgatgag cacacgaacc ggccacggtc
     3121 gcagcgccgg acgaagcagc agcaaaaagt tcagatacac cgtgatgtgc agtgccacaa
     3181 acagcggcca gatcggctga aacatggggg ccaattccgc cgcgatcagc gaatgcagtc
     3241 cgacgatgat gcccgcaaag gtgccaaacg gacgacctcg cacaagcaca gccaaaccga
     3301 cgaccgccat ccaggcacac agcgtcacca ccccaaagga aaccggcata gacgctagga
     3361 tgccgcaatc tgggtcaatc atcgcaggtt ttcgcgtggc tgcgtgccga gcgggcgaca
     3421 gcagccgcgt gccgtgatag gaaaagagca tgtctgcgca tgtgctggtg gcggatgatg
     3481 aagcgctgat cctgcaatcg ctgcggggtg tgcttctgca agagggtttc gaggtgacgg
     3541 cggccagctc gggcgagcag gcgtggcaga cgtttgtggc gaccaggccg gatgtcgtgc
     3601 tgctcgatct ggtgctcggg gacatcaccg ggatcgatgt gctgcggcgg attcggcagc
     3661 tttcgccgga aaccaaagtg attctgatca gcgcccacgc gacgctggaa caagccgtcg
     3721 aggcgatgaa gctcggtggc tacgacttca ttcgcaagcc gttcgagctg gaagagattg
     3781 tcaccaccgt tcgcaacgcg gcgcggacca gcgagctgga agggcgagtc gattacctgg
     3841 cgcgtctgac ccggcagcaa gcggattcgg gcggactttt gtatgcgtcg acgcagatgg
     3901 caaacaagct gcgggaaatt gatgtcatcg cgcgcagtcc cgtgcagacg gtgctgattc
     3961 gaggggaaag cggcaccggc aagcagctcg tcgcgcagct tctgcacgaa cgatcgacgc
     4021 ggcgcagcgg accatttgtg gaactcaact gctcgacgct gcccgaaacc ctggtggaaa
     4081 gcgagctgtt tggtcatgaa cggggcgcgt tttccgatgc gcgcgaacgc aagctggggc
     4141 tcgtcgaaat tgccgatggt gggacgctgt ttctcgatga gattggcgac ttaagcctga
     4201 gcgcccaagc caagctcttg acgtttttgg aacagcggac gtttcggcgc gttggggcga
     4261 cggcgctgcg caaagtcgat gtgcgggtga tcgcagcgac gaaccgcgac ttgaaagcga
     4321 tggcccaggg caaggaattt cgcggcgatc tgctgtttcg cttaaatgcg atcaccgtcg
     4381 agctgccgcc gctgcgtgag cgacccgatg acatcgggct gcttgctgag cattttttgc
     4441 accaggcgag tcaggagtac cacaaaaact ggcagcgatt gtcgacggaa accaaggact
     4501 tgctgacgag ctacggctgg cccggcaacg tgcgcgagct caaagcggtg atttcacgcg
     4561 ccgcgctttt gtacgacgaa cccgagctgc gtcctgtgca tttgcccgtc gagcttgttc
     4621 aatcgggtcc gccggtgcgg cgtgagacaa cgcagccggc agaactttcg cccgatcgtc
     4681 ctgcgggggc cggtcctgat ggtccgattc caacgctggc ggagatcgaa ctggcccaca
     4741 ttcggcgggt attggagctg tgcgggggaa atcgtaccgt cgctgcgcag caccttgggg
     4801 tgacgcggca gacgctgtcg cgcaagctgg aggaaggtgc ggtgtcgtaa ccgcggcctg
     4861 tgtgaatgcg ggaactattc gagcgcgcgc cgacgggcca ggtcttcggc actgtgcagt
     4921 tcaatgtcca aatcgcgcag tctgccgtcg tggatgtcgt agatccagcc gtgcaccgac
     4981 acgcgctgcc cgcgctccca cgccgcttcg agcactgtgg tttctgcgac gtttaacacc
     5041 tgctcgatga cgttcagttc acagagccgc gcggcgcgtg catcgtcggt cggcatcgac
     5101 gccagctcga tcttgtgatg acgcgcggtg tcttgaatgt ggcgcagcca gttttcgata
     5161 aggccaatct gcttgccctc cagcgcggca cgtacacccc cgcagccata gtgtccgcag
     5221 acgatgatgt cgcggacttc gagcacatcg acggcgtatt gcagcaccga caacatgttg
     5281 aggtcggtgt ggatcaccaa gttggcgacg ttgcgatgga caaacacctc gcccggtgca
     5341 agtccggtga tctggtttgc cggcacacgg ctgtcggcgc agccaatcca cagatagttt
     5401 ggtgtctgct gctcggccag tcgcgaaaag caatccgggt cccgctcgac catttcttcc
     5461 gcccagcggc gattttggac aagcaggtgc tgtagcgatg acatagaact cccccttatc
     5521 ggaaagtccg atcatacgca aacagaccag cgaaaatgtt ggcggcgtct agtgccaagg
     5581 aacgacggga gaatccgtcg cttggactag gccgtagcgg aaacggtttt gcggcgggct
     5641 gacagttggt ggtttgccag cggctcgtgc tcgtccagtc cgagcagatc gagcgagcgt
     5701 ccctgtcgct gccagtcttc ggccatgtga tgcagttttt cgatcacggt gtggtcgacg
     5761 agccgcgttc cttccagatc gagctggagg gttttgatcg tggctggcac cgcatcaatg
     5821 cgctttttga gagacaggta gttgctgaaa attgcagcgt catacacctt gaaccgcagc
     5881 gtcgattcgt cgatgacctc ttcttcaatt cgcgtggaaa acagtccgcg cagcggcgcg
     5941 ccatgacgca gatgcagaac cagtttcagc aaaacgcccg cgccgacacc gaccagcaga
     6001 tcggtcgcca gggtggtgac aagggtcgtt acaaacagca gcagctgttc tgggccgatg
     6061 cggaagactt tgacaaattc cgacggcgac gcgagccgca cgccggtata aatcagcatc
     6121 gcaccgagcg ccgcgagcgg aatccgctgc agcagacccg gcaggagcgc gaccattccg
     6181 agcagaaaca gaccgtggaa gaagttcgcg tagcggctct tggcgccact gtttatgttg
     6241 gccgatgagc ggacaatttc cgaaatcatc ggcagacccc cgagcattcc tgcgatgacg
     6301 ttgccgacgc ccgtggcttg caggtcgcgg tttaggtcgc agtgccggcg ctcttcatca
     6361 agcgagtcca ccgctttggc gctgagcagc gattccagcg aacccaccag cgtgaacatg
     6421 acgatgtact tgatcgaatc ccagttgagc agataggaaa aatccggcgg cgagtacgct
     6481 ttggcgaggc tgccgggaag ctgcacaagg tatttgggac cgactttgta ttcgtgcaga
     6541 tgaaacgtat aggtgtgttc gtggccgagg tcgaggaaca tcccgagcgg aatcgcgatg
     6601 agcagcacca ccatcggtgc cgggactttt ttggcccacg gaaacggcag cttgggaatg
     6661 ccaaacagca gcagcaggct cagaccaccg atgagcgcga cctcgggatt catcgtcatc
     6721 aagctgtgcg gaacttcggc caacagatgc agcggctcac gcgccgtcgg tgccacaccc
     6781 agcacggtgt gcgcttgttt cgatacgatg atgattccga tggcggcgag catgccgtgc
     6841 accacggcag cgggaaacgc atcacccagc acgcctgcgc gcagcctcgc tagcaccact
     6901 tgaatcacac cagcgacgac gatgaccgcc aacgcgcggt gatagccagc caccggatcg
     6961 ccatttccaa gttcttgaac ggcaccgagc gcgatcacga tcaaaccggc ggctggacct
     7021 ttgatggtga gctcggcgct gccaacgaat gtggcgatga ttccaccgac gaccgccgtg
     7081 acgatgccgg caatgggtgg gaagttgctc gccatcgcaa tgcccagaca caagggcagg
     7141 gcgatcagaa agacaagaaa ccccgagacc aagtcggcct tgaggtcggg tttgtatacg
     7201 gctgtgcggc tcatcgcggc atcctctgga agtccaaaag tgatccggcc tgagccattg
     7261 ccagcgctgt gccaaccgcg cgcggtttat tgtttggtcg tttccgtcga gagttttgcg
     7321 aagtaggaat gccggcgatg tcggcagcgg ctcgtcgtta agaaatcgaa cacgactgtg
     7381 attcggtcgg tcgattctgt aacgtgcgcg tgactaccag cgcacggtga tggtggcgct
     7441 gttgcggggg gcttgggtgc tgacttcggg cgccgagact tggcctttga catggaggaa
     7501 cgcaaaaatc gcccccattc cgccggccac ggcgacaaac acgcagtacg gaatgttggc
     7561 gctgtgacgg tagcagatgg tcatcacgcc cgacttcggt cccgtcgcgg tgtactggta
     7621 gtcgacgcct cgtaccgatg tggcggcgac ttggcccatg cgttcaaaca gcgtggccgg
     7681 ggacgttccg aacagtcgca gcatgccttc tgccatcgtg cgcagcaccg tcgtgccggc
     7741 ggtctcggat gtctctcggc tgcagcgagc gagcacatca ctgccacgca gatcgtaaat
     7801 cgcggcgtcc agttcaacca gcgcgctgcc ggcaatccag ttggacgccg ggggtggttc
     7861 gtctagcagt tttcgcgcgg gatcggacag cagcgcgcgg acttcggaca acgagcagtg
     7921 tcgcgaaagc gccttgaccc gaccgttcca cacggttcct ttggcttcaa actgcgcact
     7981 gcacttttga gcaagctcaa tttgactggg catacgaatc ccatcccaaa cgtattttcg
     8041 gatcgagcgg caccccaccg caagcgcttg tcgcgatcgc gagcaccgtg gcggcttccg
     8101 tcgcgatcct ccgcggcttc tttttggcca acttcccagg tggacagccg caccgtatcg
     8161 cctaaagatc atgccgagtg ctcgccgaac agaaacccta ggaggggaga gatcatgagg
     8221 tttgaccatc tgctgtttgc tgcttcgctc gctgtgtgtt cctcgctcgc tggctgtgaa
     8281 tcgccgccga aggggggccc agacatgtcg ggggggggat cggtggtgag tccggaacag
     8341 gcgtgtcctg cgctcatcaa ggcgtactgt gcgaaagcca cggagtgtat gccgtttaat
     8401 caggccctcg gattccccga tgctgcaacc tgtgaagcgc gcctgaacct gacttgtcca
     8461 cagcttgcaa aagcgatggg tacgtcggtg acaggcgatg atttgcaagc ttgtagcgca
     8521 aaattttcga gcatctcttg ttacgactac ctttcgctca gcaatccggt gcaggtctgt
     8581 gactttaagc caggcaagct caccgacggt agcgcttgcg gcgaaggcgt ccagtgccag
     8641 agtctgtatt gcaaaaaaga ctccacgatg gactgtggaa aatgcaccgc gcttggcaag
     8701 tccggcagcg tgtgcgtcac caatagcgac tgtgacaaag gacttgcgtg tgtgggggct
     8761 gccggggcca agcagtgcac cgcatatctg acgatgggcg caagctgtag caccgcggca
     8821 aacagtccgt cttgtcatcc gacgctggct tgtcgaaatg gtgtttgtgc cactccggcc
     8881 aagctcggag aaagctgctc agcgacgacc caggactgcg acaaactcgc cggtttgact
     8941 tgtccgctgg cgctcaaatg tgtgggctat gcgacggcgc agctgggtgg cgcgtgcggc
     9001 cagcaggggt cgacgtacat cctgtgtgcg ggcggatcct ggtgtgattc gacatccaaa
     9061 aagtgcatgg cacccgcgcc ggatggcatg gcttgtagcg acgccacggg acccaagtgc
     9121 caggccccgg caaactgtcg agccggcatc tgcaaaattg cagattctgc aacctgcatg
     9181 taacgcaaaa aagcgcacac tttggcggaa accgatgggg ggagaaatgg caaaccaaga
     9241 tgaaacgatc gctctcccgt tggctgctcc ctctctcttt cctcctcggt tgtggcggaa
     9301 cgcccgcttc gcctccgcag tggtattaca cctgcggtga tccggtctgt cgcggctata
     9361 ccgggacggc gggcgtcgcc aagtgcacca ccgaaaaagc gggcgcatcc tgcacgatgg
     9421 aaggtgtcga gtgcgatccg caggactcgt gtaaccgcaa gctgcgctgc gcgaccagcg
     9481 acccaaccca acagacgggt ggctgcccca tttcacgtcg ctcggcaaag acagacattc
     9541 agtatcttgg cgacaccgag ctgcacaagt acgccgatga agtgatgcag atgaagctgg
     9601 cgacgttcaa atatcgcagc ggtggacctg tgcggcttgg ttttatgatc gacgacaatc
     9661 cgcagagcaa gagtgtcgac ccagaacgcg acatggtgga tctgtacgga tacaccagca
     9721 tgacggtggc ggccctgaaa gtgcagcagc agcaagtcag tgatctccag aaacggctcg
     9781 atgacttgga aaaacagctc aagagctgtc gatccgcgca gccggcggtt tcggtacaca
     9841 agcgcaaata agaggtatat aaaactcctc ttatttggcg attccggaac cgaacatgga
     9901 cggccatggc aatgctgcgt taccatcgcc ccatgcagat gctgccggtg tcgcgcagag
     9961 tccttctccc tacgctggtt ttcggcgtgg cgcttctgac ccaacggggc gcgctcgctg
    10021 atttggcgcc gcgtcctcgc gtgtcgatcg cggttctgga ttccgcccac tgtccgatgc
    10081 ctgcgctgaa aaacgcggtc gaacaggtgg aggctgaact ggaatcgtgt gcgaaagagg
    10141 aacgggcacg cggcaagacg gtgcttcact gctcgtttga caagtcaggc cacgtcaaat
    10201 cgtgcagtgt ggcgtcgaga agcgggcatt ttagcaaagc gcaaaacgag tgttttcgca
    10261 aagcgctcgt gcaggttcag atcccgcttg ccaagtggga tggcgggagc aaacccagtc
    10321 tgtgtaccgc gcaggtccaa gtggagtcgg tgattccgcc ataccgtcgg ccccgtccac
    10381 acgggtccgg agacatcatc ccagattttt agttacagga tgccgcgctg gcggatttca
    10441 ttgatggcgg attgaagcgc attgcctttg cgcagacacg gccagcagtc cgtctgggca
    10501 cctggaccgc atcccgtcgg tatttgcagc ttcagcagcg tttctagcgt gcgtttgccg
    10561 ccttgcttgc ccgctttggc gaccagtgcc tttttcgctt cgcagcttgc cgcattgcgc
    10621 agatcgagca ccatcacacc atcgggagac gccagcttgc ggacggcttc tttgctcagg
    10681 ctcatcgtca gtcgaccgcg atagggctcc atcgtcgtgc ggtcggccag ctcaatcagc
    10741 atgtcgacgc cgtattcgcc catcttttgt tcgagcagct gaaccgccgg ctccgaggtc
    10801 tgcggctgca gcgctgcatc ggcaaccagg cgcaggattt gatcatcgta ggcggcggct
    10861 ggatcgacgg tgagcagcgg aggaatcgct tgcaacgccg atcccagctc gcccgctttc
    10921 ccatacgctt tgaccagcgc ccgatgggtg cgcgagtcgt tgggatattt gttgcgtagc
    10981 ttgagcagtg ccgccgggcc tttttcgacc gctttttgca tgtcggcatc gctggcaaaa
    11041 ccgaccattc cgaccaccgc cgagtcgctg tcttcgtcgc tgccgatcag cagtccgaac
    11101 agaaaaaacg tcggcaagag cagcgccgcc gcaaccgcag ttcccagtgc ccactgcgaa
    11161 acgccccggt gtttttcggg cagcttgccg cggacaaagc ccaaaagagc cgaccacttt
    11221 tgactgatcg tcgacaacac gagggctagc ttggctggga gtccgcccgc cgaacccgcc
    11281 ggcacaattt cattgctcga agcagaaagc gccggcaccg ttgcagcgtg tccgacggcg
    11341 cggttggctt cgtctttatc acgggcgctg tcttttcctg cggggagcgc cggatgtttg
    11401 ccaccactgt ccccggcttt ggttgccgga acgccgccgg ttgctttggc cgaagccgcc
    11461 gcgccggcta ccgccggaat ctgcacagcg ctcgggctga caatttcgat ctgcgccacc
    11521 gattcgccac ccgttgcggc ggcttgggcg ggatagtctt tgtcgcccat caccgtcggt
    11581 cggctgtcga tgtcacggat tcccgaattc ggaatgttca gccggccaag cggcgtcgac
    11641 ggttcgtagc gcagcccttc cgccatcgcc agctccgtca gcgcgtcgag cagtgcacgc
    11701 gcatccttgt ggcgattgtc gggatttttt tcgagcagct tcatcaccat cgcttcaagc
    11761 gtcgccggaa ctttcaccga cggggctttc tgcgccattg gtggcacagg cgccaggatc
    11821 tgctgtcgca aaatcaccgc tggatcgttg gcatcaaacg gcacccagcc ggtcagcagc
    11881 tcgtacatca cgacgcccag cgcataaaga tcggtgcgtc catcgacctc accgccggca
    11941 gcttgctcgg gtgccatgta cgctggcgta ccgaaaatcg ttccgtattt cgtcagcgtt
    12001 tcggatcgct ggctgacatc ttcaacgagc gcttcgatgc gaatcttggc caagccgaag
    12061 tcgagcactt tcacgaagtc ggggtcgccg tcccggttca tgatcatgat gttttcgggc
    12121 tt
//
LOCUS       DAORGN010000153        12069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2207_length_12069_cov_6.958632, whole genome shotgun sequence.
ACCESSION   DAORGN010000153 DAORGN010000000
VERSION     DAORGN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_12069_cov_6.958632"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..428
                     /locus_tag="PKL17_15470"
     CDS             42..428
                     /locus_tag="PKL17_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HNK46184.1"
                     /translation="MFEGETRAKQGDSVGWHLESSGDKEKGTYILEGTRSPTDEHGVY
                     EGNVMIEGLKKKARSTFFPKDWTEKQVETAIGEAYRNKRLDEKSGRYRGEDAKGVEIE
                     MMLNHKGDIDSAYPVYKGPKYEGPKK"
     gene            425..820
                     /locus_tag="PKL17_15475"
     CDS             425..820
                     /locus_tag="PKL17_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46185.1"
                     /translation="MRVVATAMPGSDSAFAAVVDERDGVRHRLLNGFLADSERNEDLY
                     LAALDRAEAGTPELYWTNHYVDADMYPDGRVVITKTERLLEGEEKERPPQIVITIGEA
                     RKLILDWIEARDKWYADREAQRAAAGQSK"
     gene            complement(869..1900)
                     /locus_tag="PKL17_15480"
     CDS             complement(869..1900)
                     /locus_tag="PKL17_15480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46186.1"
                     /translation="MPPLLQEGQIVRNHRIGRRIGHGGMGEVYEAAHVSLQRRVAIKV
                     LHPDWCKNPDLVKRFFNEALATNLIRHPAMVEIHDHGKFPDGMPFLIMEFLEGQSLRR
                     YRGTLQPLQVVRLAWQVASGLRAAHAKKIVHRDLKPDNVMVIRDDTVPGGQRVKILDF
                     GLAKLAAEHQQEGAEAVKTRDGVALGTPEYMAPEQWLGASKVDGKADVYSLGVVLYET
                     IAGTPPFPSSEGSRLRSLHLYTPPPSLAEKSPDTPKELVKLIEDMLIKTASERPAMAE
                     VEQRLETMLRSLDRSDPPLNLEPQPPAKPDPPPSPAPSSTWLPLLASAAVGFFLVSLL
                     LLFFLLTRH"
     gene            2008..2754
                     /locus_tag="PKL17_15485"
     CDS             2008..2754
                     /locus_tag="PKL17_15485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HNK46187.1"
                     /translation="MPWVARRLRDAKVFVACDKNGEPSVGPDGRIDMLYRAGGKIYRA
                     AARNLTAGEEPTLRSDEEMHPAGRAEDDASKTAAPAATSAKTKTGASKTPAALGHGKH
                     PHLSAQAQADAVHIYTDGACTGNPGPMGLGVVILDPAGPKGEPQRRELGEYLGTGTNN
                     IAELTAILRGLSLTPRDRAVVIYTDSSYSIGILSQGWKAKANQELVAEIRTLLREFPQ
                     VRFVKVAGHAGIPENERCDELARQAILRRS"
     gene            2944..3462
                     /locus_tag="PKL17_15490"
     CDS             2944..3462
                     /locus_tag="PKL17_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46188.1"
                     /translation="MNFRNSAALAKTFRRRSVWLAGFLLCFVGLSGCVTLRPIPGTKI
                     ADTPLHRELLQRVEEYRIAMEQRDAGKLLSMAHPNYYEDSGTPSAQDDYAYAGLKRVL
                     ETRLSSVRWMRYLIRYRDIRVTGDRATVDLRYDLSFQLMTEIGEKWERRQNDKRLELV
                     HEGDRWLFTSGF"
     gene            complement(3446..4345)
                     /locus_tag="PKL17_15495"
     CDS             complement(3446..4345)
                     /locus_tag="PKL17_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46189.1"
                     /translation="MLTPLLLALVLLPDVVMPRPTELANLAGDSVELERQAHRLGPAR
                     LLRLLQRPQSDKGRLAFLALRGLSLVATQHPEVAGQAALPLLDLLEQAGDDKLRHALS
                     DTLLRVCQVLGRSLPCDEPDDLGCGGDLASLPLRLVQLAEKPSQPVLSRSVALSALQA
                     LPFQVWQTSSPRLFALAQRESGPVRTTVLAALSVLHQQAGKPELLTLATLPDDSLAAA
                     ASQELCWPLLAHKPNAKPSAAPAAAVVPDAVLRRAREIAAGTQPMAVRLQTIECLRAT
                     GLAQDKALLLQLATAAKAAKNRK"
     gene            4512..5213
                     /gene="lexA"
                     /locus_tag="PKL17_15500"
     CDS             4512..5213
                     /gene="lexA"
                     /locus_tag="PKL17_15500"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNK46190.1"
                     /translation="MPRSKTPPSPSSSPADSRRPLTSRQQEVLSLISESIEQRGYPPT
                     VRELGDRLGIRSTNGVNDHLVALAKKGYLERQDLKSRALRVVTAEPADVPPTHSGDTV
                     EVPVLGRVAAGQPILAQEHHEDRLRVDRLLIGNHREVFALRVQGDSMIEAGIYDGDFV
                     FVRKQPMANPGDIVVAMISDEATVKYYHPEPDRIVFKPANSRLQPIYVRRDEFRPVNL
                     LGTVVGLYRRFSPTN"
     gene            complement(5270..6712)
                     /locus_tag="PKL17_15505"
     CDS             complement(5270..6712)
                     /locus_tag="PKL17_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNK46191.1"
                     /translation="MRKTKTVTLFGLSLLGMSVLGCASADAPVPATHAAPLTASYSWD
                     GDLPRLRDATEADCHFESEKLVLRGATLYRVYKVRYRSLESRGGTLSQIEIRGYAARP
                     DGGSKSPAVVLAHGLGGSADESATIGLAERLSAFVLSYTGPGGGTSPDNTSEGAPAFD
                     GSGYRMFDTLGDVRGSWFWGHTAAALRGITCLAARPEVDVRKLGLTGYSAGGVATLIG
                     TGADDRIAAGVALSGTLAWDVATDSADAWQHALLQKAGLSTASREWQKLTTELIDPAR
                     ALGKTAGKLLLIDGTTDEFFPLTALVSTVSALPPITKSEQARISLAANFDHGCYKLSG
                     VESASAIEARALLRSDGGQRMWFGNAFGTDSRFRSLPATPQVSTIPVGAGTLVTAVVD
                     EPSSLAVDEVRLFVSADRSYTYANVKLDRDGSRTYRKLLPLSLAGSVTFVDVQYKTRD
                     LLSPVRFSLSSLPSLPSGMVPHIRSQTSCL"
     gene            complement(6745..8085)
                     /locus_tag="PKL17_15510"
     CDS             complement(6745..8085)
                     /locus_tag="PKL17_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK46192.1"
                     /translation="MKIPGPSALSFLVGVRRFGFLDYVGKLWKEHGDVFSVRLGPRTL
                     VFAMHPDAVEHISVSHRQNYDKLRSYDAVRTYLLGQGLVASTGELWRRQRKLMAPFYT
                     PKGVQTYAALMLRDGERLVERWQEFAKQGTEVEIAEEMTYVTASIILKAMFSTETIDS
                     IHQMKDAVETMISFVNGQMAGFRLPTWLPTEGNRKYFAARKLVHEAIESVIAQRRSIP
                     QSNWPDDLLSKLLCARDEESGQPMSESLLRDESITTFFAGHETTARTMTFAWYALAAN
                     PLVTAQLHRELDAVLQGRSPTVEDLRRLPYTLQVIKEVLRLYPAAPFYVRDAITEDRF
                     LGFSIPAGAAVMLSPYYTHRHPWFWPDPDVFDPTRWTREKESERNGYAYHPFAQGQRI
                     CIGNNFSLLESHLLLALLAQRFTPRLRPGYAPQWTMQGTLGLVSGLPMTIALRR"
     gene            complement(8171..9733)
                     /locus_tag="PKL17_15515"
     CDS             complement(8171..9733)
                     /locus_tag="PKL17_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNK46193.1"
                     /translation="MSSRLRTSAFLLGAQLLLASCSLVLDSSVKKGIGESCAADSDCQ
                     AAKCLMSTAFPTEKTGVCSLQCSSASDCPAPSSCIKSQCQLQLTAGVAMTGFPGELEG
                     WTVAHNDGLQTASDALGYLKLEKKFGLTPGNVLSDVKDLASRNQVVIGNTVDYGDDFV
                     AAAKLYPDKKFLVVNDGVRYDYPGRTSNFSTYWVHREQAWYVAGKVAATIGKKRLGVI
                     SAFINPDCIRDVNAFALGARSVNPNIVVEVRHMGFWFDINTSPTYSYPPISPSTVNYR
                     EEYLTRMMWDSGVEVIAHLGNTQRSVKLIDQLMAQNPSRPTKVWTFANDVKTGCKDST
                     GAYFPTCIGSIYENWAPIYTRIFDQIQRGVYTAATVEFDVDGTDNTSVGVSINPKGPG
                     DDLATRALIQALAQATNPGPRYRVFSGPYDINGQRDKDGDGIPDATQKMLSGETVSDT
                     ELARMCWFVKGVVEKTKLNDPTSADRDALVPGGLIPGSSTPNQLQSYVAESEDKLVLP
                     TGLSAKCRENSF"
     assembly_gap    9791..9890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9891..10805)
                     /locus_tag="PKL17_15520"
     CDS             complement(<9891..10805)
                     /locus_tag="PKL17_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46194.1"
                     /translation="MAQEKEDAAGSDICIADAICRAHYSRARKLSKKDDYEGALEAYE
                     AAYRRKPAQWLLINMGRTLHKLGRTQDAMDQYRRYLASSDANADLRKKAEQFLTEAEA
                     DVAARPKKEPAKTQASDPAQALPGNLPPSSTVNSPDRSTTPSDPATDKRDDKAKTLKP
                     IDVEPVAPASYKEPPPSRLGPLFYGGIAVGGALVVGGVITGIIGLSSAKTLQATPYAG
                     ALDKTTLPDLQKRVQNLGYATDVMIPLGVATIAVTTIVSLVRKPASEKTATPAAKNSQ
                     PPSIPATHSPPPPPAPPSTPAPAASVQPS"
     gene            complement(10861..>12069)
                     /locus_tag="PKL17_15525"
     CDS             complement(10861..>12069)
                     /locus_tag="PKL17_15525"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46195.1"
                     /translation="MLVRDPAVPGGERVKLLDFGIAKLRDPNLRKNLTKGDSLLGTPA
                     YMSPEQCKGGVDVTDRADVYSLGIILYRVLAARLPFLATGGGELLGMHQFQTPTPLSE
                     VAGYLPQSVVALVEEMMRKDPLTRPSMSEVDQRIAGLQTELSGFVQPIFPIAEPDAES
                     MDDDEHEAATIDASSSDAEMPLNVVFAPLMTPTTPTTPGVPSRPIGLDESGSQGYRST
                     MNMAASENSSPQSHLLERHPSLRWGLLSVVLLCIIGGVTLLSRPNPKQTAQSNQSTTP
                     KVEEPSKTPAAPPRRVQWQLRTSPVRADVLRVSDGALLGKTPWQSEQPVGQGSQELRL
                     IAPGYADKLVVLDLSRDEQQSLDLEPIAAKGSPIPGTKTEAQNGKSSQKALKGDAQKK
                     KKKQQIEIEE"
BASE COUNT         2495 a         3684 c         3505 g         2285 t
ORIGIN      
        1 aggaagcgtc agacatcctg accgagcgga gccgcaagca catcttcgag ggcgaaaccc
       61 gtgcgaagca gggagattcg gtcggttggc acctagagtc gagcggcgac aaagagaaag
      121 gaacgtatat tctcgaaggc actcgcagcc cgacggacga gcacggcgtg tacgagggaa
      181 acgtgatgat cgaagggctc aagaaaaagg cccgctcgac gtttttcccc aaagactgga
      241 cggaaaagca ggtcgagacg gcgattgggg aagcgtatag aaacaagcgg ctggatgaaa
      301 aatcgggccg atatcgagga gaagacgcca aaggggtcga gattgaaatg atgctcaatc
      361 acaaaggcga catcgacagt gcgtacccag tctacaaagg ccccaagtac gaaggaccga
      421 agaaatgaga gtcgttgcaa cggccatgcc gggttctgac agtgcatttg ctgccgtggt
      481 cgatgagcgg gatggcgttc ggcacaggct gctcaatgga tttctcgcgg acagtgaacg
      541 aaacgaagac ctgtacctgg cggcgttgga cagagcagag gcagggacgc cggagctgta
      601 ctggaccaat cactatgtcg atgcggacat gtatcccgat gggcgggtgg tcatcaccaa
      661 gacggagcgg ctgcttgaag gtgaagagaa ggaacgcccg ccgcagattg tgatcacgat
      721 tggggaggcg cggaagctga tcctcgattg gatcgaagcg cgggacaagt ggtacgcgga
      781 tcgggaagcg cagcgagcgg cggcgggaca gtcgaagtag gccgggcggg aacacttgcg
      841 tgttggcggg cgcggttggc caaacgttct agtgacgagt cagcaggaag aagaggagga
      901 gcaggctgac caggaagaaa ccgacggcgg cacttgccag gagcggcagc caggtgctgc
      961 tcggtgctgg ggacggaggc gggtcgggct tggcaggcgg ctgcggttcc aagttgagcg
     1021 gcggatcgct gcgatccaag ctgcgcagca tcgtctcgag ccgctgctcg acttcggcca
     1081 tcgccggacg ctcgctggcg gtctttatca gcatgtcttc aatcagcttg accagctctt
     1141 tgggcgtatc gggcgacttt tccgcaagtg acggcggcgg cgtgtaaagg tgaagtgagc
     1201 gcagccgcga gccttcgctc gatggaaaag ggggcgtccc ggcaatcgtt tcgtacagca
     1261 ccaccccgag cgaatagaca tcggctttgc cgtcgacttt gctcgcgcca agccactgct
     1321 cgggtgccat gtattcgggc gtgcccagcg ccacaccgtc gcgcgtcttg accgcttcgg
     1381 cgccttcttg ctgatgttcc gccgccagct tggccagccc aaagtccagg attttgaccc
     1441 gctgcccgcc aggaaccgtg tcgtcgcgaa tcaccatcac gttgtcgggc ttcagatcgc
     1501 ggtgcacgat ttttttggca tgcgccgccc gcagccccga cgccacctgc cacgccaaac
     1561 gcaccacttg caaaggctgg agcgtcccgc ggtagcgccg cagcgactga ccttcaagaa
     1621 actccatgat cagaaacggc atcccatcgg gaaacttgcc gtgatcgtgg atttcgacca
     1681 tcgccggatg gcgaatcagg ttggtggcca gcgcctcgtt aaagaagcgc ttgaccaagt
     1741 cgggattttt gcaccagtcc ggatgcagga ctttgatggc cacgcggcgc tggagcgaga
     1801 cgtgcgcggc ctcgtagact tcgcccatgc caccgtgccc aatgcgccgc ccgatgcggt
     1861 gattgcggac aatctgacct tcctgtagaa gcggcggcat acgctcagag tacatcaagg
     1921 gatgcgcaga ttggctagcg cgaaacggtc cgtggcgagg gccggcggct cttgccaagc
     1981 gacgcggtga cggctagggt gcgcagcgtg ccatgggtag cgcggcgtct gcgagacgcc
     2041 aaagtcttcg tcgcctgcga caaaaacggt gagccatccg tcggtcccga cggtcggatc
     2101 gacatgctgt atcgcgcagg cggcaaaatc tatcgggccg cagcccgcaa tctgaccgca
     2161 ggtgaagaac ccacgctgcg cagcgatgaa gaaatgcatc cagcgggacg ggccgaagac
     2221 gacgcgagca aaaccgccgc gccggcagcg acttccgcca aaaccaagac tggtgcgagc
     2281 aaaacgccag ccgcactggg tcacggaaaa catccacacc tgagcgcgca ggcccaagcg
     2341 gacgccgtac acatttacac agacggcgcg tgcaccggaa atccgggacc gatggggctt
     2401 ggcgtcgtca tcctcgatcc cgccgggccc aaaggggaac cgcagagacg ggagcttggc
     2461 gaatacctgg gcacgggcac caacaacatc gccgagctga cagccatcct gcgcggactt
     2521 tcgctgacgc cgcgagatcg ggcggtcgtc atctacaccg actccagcta ttcgatcggg
     2581 attttgtcgc agggctggaa agccaaagca aaccaagagc tggtcgccga gatccgcacg
     2641 ctcttgcgcg aatttcccca ggtccgcttt gtcaaggtgg caggccacgc cggcattccc
     2701 gaaaatgaac gctgcgacga gctggcccgg caggcgatct tgcgccgcag ctaacccaag
     2761 cgaccacgca gacgcgacat ggccgaagtg acaagctgac aatctttgct ggtgtcacgc
     2821 tgacatgatg acgcagcaca agtcgctgct cgacggggac gatctggcac ctgctttgca
     2881 ctttcaccga ctgaaagccc aacgggtcgc ggtaagctgc ccaggctttt gcaaggatca
     2941 cccatgaact ttcgcaactc cgctgctttg gccaagacct tccgccgtcg ctctgtttgg
     3001 ctggcaggct ttctgctgtg ttttgtcggg ctgtcaggct gcgtgacgct gcggcccatt
     3061 ccggggacca aaatcgccga caccccactg caccgcgagc tgctccagcg cgtcgaggaa
     3121 tatcgcattg cgatggaaca gcgcgacgcg gggaagcttt tgtcgatggc gcacccgaac
     3181 tactacgaag actcgggcac accgtcggcg caagatgact acgcttatgc tggtcttaag
     3241 cgggtgctgg aaacgcggct gtcatcggtg cgctggatgc gctacctgat tcgctaccgg
     3301 gacatccgtg tcaccggcga ccgcgccacc gtcgaccttc gctatgacct aagctttcag
     3361 ctgatgaccg agattggcga aaagtgggaa cgtcgccaaa acgacaaacg gctcgagctg
     3421 gtccacgaag gcgaccgctg gctgttcact tccggttttt agccgccttc gctgccgtcg
     3481 caagctgcaa aagcagcgcc ttgtcctgcg caagtcctgt cgcccgcaaa cattcaatcg
     3541 tctgaagtcg cacagccatc ggctgcgtac ccgcagcaat ttcgcgggcg cgccgcagca
     3601 cagcatcggg aacaaccgct gcagccggcg cggcagacgg cttggcgttc ggtttgtgcg
     3661 caagcagcgg ccagcacagc tcttgggacg ccgccgccgc caagctgtca tcgggcagcg
     3721 ttgccagcgt aagcagctcc ggttttcccg cttgctgatg caagaccgac agcgccgcaa
     3781 gcacggtcgt ccgcaccggc cccgactctc gctgggccag tgcaaaaagc cgcggtgacg
     3841 aggtctgcca cacttgaaac ggcagcgctt gcagcgccga cagcgccaca ctgcgcgaca
     3901 gcaccggctg actgggtttt tccgcaagct gcacaagccg cagcggcaag ctggccaagt
     3961 cgccgccaca gcccagatcg tcgggctcat cacacggcag cgatcgcccg agcacctgac
     4021 agacccgcaa cagcgtatcc gacagcgcat ggcgcagctt gtcgtccccc gcttgttcca
     4081 acagatcgag cagtggcagc gcagcctgac cggcgacttc aggatgttgt gtagcgacga
     4141 gcgacaggcc ccgcagcgcc aaaaatgcca gccgcccttt gtcgctctgc ggcctctgca
     4201 aaagacgcaa aagccgcgcc gggcccaggc gatgggcttg ccgctccagc tcgacggaat
     4261 cgcccgccag attcgccaat tcggtgggcc gtggcatcac cacgtcgggc aaaagcacaa
     4321 gcgcgagcaa aagcggcgtc aacatgggcg atagcctgca caacacacga cctttttatc
     4381 ttcgcgggaa gcgcgcgatt cgtaaagcac gtcggcaaat ccccacccag acgcctgcca
     4441 tgcgcagact tgattgacga cttgggcctg ggaaagtagc ctcacattcg ttcagtcttt
     4501 tcgaggttct catgccccgc agcaaaacgc cgcccagtcc gtcctcctcg cctgccgact
     4561 cgcggcgacc gctcacgtca cgccaacaag aagtgttgtc tctgatcagc gaatcgatcg
     4621 aacagcgcgg ctatccgccg acggtgcgcg agctgggtga tcggctcggg attcgatcga
     4681 ccaacggtgt aaacgaccac ttggtggcgc tggcgaaaaa aggctatcta gagcggcagg
     4741 atctgaagtc gcgcgcgctg cgggtggtga cggcggaacc tgccgatgtg ccgccgacgc
     4801 actccggtga caccgttgaa gttccagtgc tcggacgagt cgccgccggc cagccgattt
     4861 tggcgcagga acaccacgaa gacaggctgc gcgtcgatcg cttgctgatc ggtaaccatc
     4921 gcgaggtgtt cgcgctgcgg gtgcaaggcg actcgatgat cgaggccggc atctacgacg
     4981 gggattttgt gtttgtgcgc aagcagccga tggccaatcc cggtgacatc gtggtcgcga
     5041 tgataagcga tgaagcaacc gtgaagtact accatccgga acccgatcga atcgtgttta
     5101 agcccgcgaa cagccggctc cagccgatct atgtccgccg cgatgagttc cgtccggtca
     5161 atctgctcgg tacggtcgtc ggtctgtatc gccgcttcag ccccaccaat tagaacgccg
     5221 caaagaccga ctgcggaccg tctgggcccg accgtcttcc atcacaatgt cacagacagc
     5281 tggtctggct gcgaatgtgc ggcaccatgc cgcttgggag actcggcagc gatgacagcg
     5341 aaaagcggac tggcgaaagc agatcgcgcg ttttgtactg cacatcgaca aacgtcacgc
     5401 tccccgcaag cgacagcggc agcagcttgc gataggtccg gcttccgtcg cggtcgagct
     5461 tcacgttggc ataggtatac gatcgatccg cgctgacaaa gagccgaact tcgtcgacag
     5521 caagcgagct gggttcgtcc accactgcag tcacgagcgt gccagcccca acgggaattg
     5581 tggaaacctg cggcgtggcg ggcagactgc gaaaccggct gtcggtgcca aaggcgttgc
     5641 caaaccacat gcgctgtcca ccatcgctgc gcagaagtgc acgggcttcg atggcggacg
     5701 ccgattccac accgctgagc ttgtagcagc cgtgatcgaa gttggcggcc aagctaatcc
     5761 gggcttgctc gcttttggtg atcggcggca gcgcggacac cgtcgaaacg agagcggtca
     5821 gcggaaaaaa ttcgtccgtt gtgccatcga tcaaaagcag cttgcccgcc gtcttcccga
     5881 gcgcacgcgc cgggtcgata agctcggtcg tcagcttttg ccactcgcga ctggctgtcg
     5941 aaagtcccgc cttttgcaac agcgcatgct gccaagcgtc ggcggaatcg gtcgccacat
     6001 cccaagccag tgtgcccgac agcgcaacac cagcggcaat ccgatcatcg gcgcccgttc
     6061 caatcagcgt cgcaacccca cccgccgaat atccggtcag tcctagcttg cgaacatcga
     6121 cttccggacg cgcggccaga caagtgatgc ctcgcagcgc cgccgccgtg tgaccccaaa
     6181 accagctgcc gcgaacgtca ccaagggtgt cgaacatgcg atagccgctg ccgtcgaaag
     6241 ccggagcgcc ctctgatgtg ttgtcagggc tcgttccccc gcccggtccg gtataggaaa
     6301 ggacaaatgc cgacagccgc tcggccagac cgatggtcgc gctttcatcc gcagatccgc
     6361 caagtccgtg cgccagcacc acagccggag acttgctacc gccgtcggga cgcgctgcgt
     6421 atccgcggat ctcgatttga ctgagcgtgc cccctcgcga ctccagcgaa cgataacgaa
     6481 ctttgtagac ccgatacagc gtcgcgccac gcaggaccag cttttcggat tcaaagtgac
     6541 agtccgcttc cgtcgcatcg cgcagccgtg gcagatcgcc atcccagctg tagcttgcgg
     6601 tcagcggtgc cgcatgggtc gccgggacag gcgcgtcagc ggaagcacag cccagaacag
     6661 acattcccag aagggacaac ccgaacaaag tgacggtctt tgttttgcgc atgcggatac
     6721 cttatccact tcgcagcgca gtcgtcatct tcgcagcgca atcgtcatcg gaagtccgct
     6781 gacaagtccc agcgtcccct gcatcgtcca ctgtggtgcg tagcccggtc gcagccgcgg
     6841 cgtaaaccgc tgcgccagaa gtgccagcag caagtgactt tccaaaagcg aaaagttgtt
     6901 gccgatgcag atgcgctgcc cttgcgcaaa cggatgatag gcgtagccat ttcgctccga
     6961 ctctttttcc cgcgtccagc gcgtcggatc aaacacatct ggatcgggcc aaaaccacgg
     7021 atgccgatgg gtgtagtaag gagacaacat caccgcagca cccgccggaa tcgaaaagcc
     7081 caggaatcgg tcttcggtga tcgcatcacg cacgtaaaac ggagccgccg gataaagccg
     7141 cagcacttcc tttatgactt gcagcgtata gggaagccga cgcaaatctt cgacggtcgg
     7201 cgatcttccc tgcaataccg cgtccagttc ccgatgaagc tgcgctgtga ccaaaggatt
     7261 cgccgcaagc gcataccacg caaacgtcat cgtgcgcgcc gtcgtttcat gtccggcaaa
     7321 aaatgtcgtg atcgactcat cgcgcagaag cgactccgac atcggctggc cgctctcttc
     7381 atcgcgtgca cacagaagct tcgacagaag atcatcgggc cagttgcttt gcggaatgct
     7441 ccgtcgctgc gcaatcaccg attcgatcgc ttcatggacc agcttgcgcg cggcaaagta
     7501 cttgcgattc ccttccgtag ggagccaggt cggtagccgg aatcccgcca tctgtccatt
     7561 tacaaacgag atcatcgttt cgaccgcatc tttcatttga tgaatcgaat cgatcgtctc
     7621 ggtactaaac atcgctttca aaatgatcga tgcggtgaca tacgtcatct cttccgcgat
     7681 ctcgacttcg gtgccttgct tggcaaattc ctgccatctt tcgacgagac gttcgccatc
     7741 gcgcagcatc agcgccgcgt aagtctggac tcctttggga gtataaaaag gagccatcag
     7801 cttgcgctgc cgccgccaca gctcgccggt actggcgacc agaccctgac caagcagata
     7861 ggttcgcacc gcatcatagc tacgcagctt gtcgtagttc tgccggtggc tcacactgat
     7921 gtgctcgacg gcgtccggat gcatcgcgaa caccaaagta cgcggaccca aacgaaccga
     7981 gaacacatcc ccgtgttctt tccacagctt cccaacatag tcgagaaagc caaagcggcg
     8041 gacgcccaca aggaatgaca gagcgctcgg acctggtatc ttcacaagat cctacgatgc
     8101 cgcaaaatga cgattttgtc gacggggatt cagcgtccga gggtgctgat gaaggaacaa
     8161 gagaaacgga ctaaaaggaa ttttctctgc actttgccga cagacctgta ggcagcacca
     8221 gcttgtcttc cgactctgca acatacgact gaagctggtt cggcgtgctg ctacccggaa
     8281 tcaggccacc cggaaccagc gcatcgcggt cggctgacgt tggatcgttt agcttggtct
     8341 tttcgacgac gcctttgaca aaccagcaca tgcgcgcaag ctcagtatcg ctcaccgtct
     8401 cacccgacag cattttttgc gttgcgtcag gaattccgtc cccgtctttg tcgcgctgtc
     8461 cgtttatgtc ataaggtccg ctaaacaccc gataccgtgg cccaggattt gtcgcctggg
     8521 ccagtgcttg gatcagcgcg cgggtcgcca gatcatcccc cggacctttc ggattgatcg
     8581 aaacgccgac cgaagtattg tctgtcccat cgacgtcgaa ctccaccgtg gcggcggtgt
     8641 acacaccacg ctgaatctga tcaaaaatgc gggtgtagat gggcgcccag ttttcgtaaa
     8701 tcgatccgat gcaggttggg aagtaggcac cagtgctgtc cttgcagccg gtctttacgt
     8761 cgttcgcgaa cgtccacact ttggttgggc ggcttgggtt ttgcgccatc agctgatcga
     8821 tgagcttgac cgatcgttgc gtgttgccaa gatgcgcaat gacttccaca ccagagtccc
     8881 acatcatccg cgtcaggtat tcctctcgat agttcactgt cgacggagaa atcggcggat
     8941 agctgtacgt aggcgaggta tttatgtcaa accaaaaacc catgtgccgg acttccacca
     9001 cgatgttcgg attcacggat cgtgcgccca gcgcaaacgc attcacatcg cggatacagt
     9061 ccggattgat aaacgcactg atcaccccga gtcgcttttt tccgatcgtt gccgcgactt
     9121 ttcccgcgac ataccaagct tgttcgcggt gtacccaata ggtcgaaaag ttcgaagtgc
     9181 gacctggata gtcgtaacga actccgtcgt ttaccaccag gaatttcttg tccggataca
     9241 gttttgcagc ggcaacaaag tcatcgccgt aatcgacggt gttgccaatc accacttgat
     9301 tccgactcgc aagatccttc acatccgaaa gcacgtttcc tggcgtcaga ccaaacttct
     9361 tttccagctt cagatatccc agtgcatcgc tcgctgtctg cagcccatcg ttgtgcgcca
     9421 ccgtccagcc ctccagctcc cctggaaagc cggtcattgc caccccagcg gttagctgga
     9481 gctgacactg gcttttgatg cacgaagaag gagccggaca gtcgcttgcg ctgctgcact
     9541 gcagcgaaca gacgcctgtt ttttccgtcg gaaacgccgt actcatcaga cacttggcgg
     9601 cctgacaatc actgtctgca gcgcaggact ctccgattcc ttttttgacg ctggaatcaa
     9661 gcaccagcga acaggaagca agcaggagct gtgcgcccaa aaggaaggca gaagtacgaa
     9721 gtcgggaaga catgatccac tccttcgaca aggttacgga gtcgggttag gagtcgggtt
     9781 aggaaccggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn actgggctgg
     9901 acagaagccg cgggcgcagg cgtggaaggg ggcgcaggcg gcggcggcgg actgtgcgta
     9961 gcagggatcg acgggggctg gctgtttttg gcggcaggcg tcgctgtttt ttcgctcgcc
    10021 ggctttcgta ccagggaaac aatcgtcgtc accgcaatcg ttgcgacgcc caaaggaatc
    10081 atcacatcgg ttgcatagcc caggttttgc acccgctttt gcaagtccgg aagggtcgtc
    10141 ttgtccagcg ctccagcata gggagtcgct tgcagcgtct ttgccgacga aagtccaatg
    10201 attccggtga tcactccacc gacgaccaac gcaccgccga ccgcaatccc accataaaac
    10261 agcggcccaa gtcgcgacgg gggcggctcc ttataggaag cgggtgcaac cggctccaca
    10321 tctatcggtt tgagggtctt tgctttgtca tcccgtttgt ctgtcgcggg gtccgatggc
    10381 gtggtggatc ggtccggact gttcacagtg gacgatggtg gaagattccc cggaagggcc
    10441 tgtgccgggt ccgatgcttg cgtcttggcc ggttcctttt tcggtcgcgc cgccacatcg
    10501 gcttccgctt cagtcagaaa ctgctcggct ttttttcgca ggtccgcgtt tgcatccgac
    10561 gacgcgaggt agcgcctgta ctgatccatc gcgtcttggg tacgtcccag cttgtgcagg
    10621 gtacgtccca tgttgatgag cagccactgg gctggctttc gccggtaggc cgcttcatac
    10681 gcttccagtg cgccttcgta gtcatctttt ttggaaagct tccgcgcccg gctgtagtgg
    10741 gcccggcaga tcgcatccgc gatacagata tcgcttccgg cagcatcttc cttttcctgc
    10801 gccatcgcag gtggcgcaaa aaccaggaac gcaagaacgg acgtcgcggc agtccagttc
    10861 ctactcttcg atttcgatct gctgcttctt ctttttcttt tgagcatcgc ccttcagtgc
    10921 cttttgcgaa ctctttccgt tttgagcttc cgttttcgtc cccggaatcg gcgacccttt
    10981 cgcagcaatg ggttccagat ccaggctctg ttgctcgtct cggctcaggt ccaacaccac
    11041 cagcttgtcc gcatacccag gggcaatcag acgcagctct tggctgccct gccccactgg
    11101 ctgctcgctc tgccatggcg tcttacccag gagtgcaccg tcggacaccc gcaatacatc
    11161 tgcccgcacc ggagaggtac gaagctgcca ctgcacacgc cgcggaggcg ccgcaggggt
    11221 ctttgacggc tcctccactt tgggcgtggt ggactgatta gactgagctg tctgttttgg
    11281 attcggtcgg ctaagcagcg tcacgccacc gatgatgcag agcagaacca cagacagaag
    11341 tccccagcgc aaagacggat gccgttccag caaatgggac tgcggtgagc tgttttcact
    11401 cgccgccatg ttcattgtgg agcgatagcc ctgcgaccca gactcgtcga gcccgatcgg
    11461 acgcgacggc acgcccggtg tcgtcggcgt cgtgggagtc ataagcggag caaacaccac
    11521 attgagcggc atttcggcat cgctgcttga tgcatcaatc gtggccgcct cgtgctcatc
    11581 gtcatccatc gactccgcat cgggctcggc gatcgggaag atgggctgga cgaaaccaga
    11641 cagctctgtc tgtaaccccg caatccgctg atccacctcg ctcatggatg ggcgggtcag
    11701 cgggtctttg cgcatcatct cttccacgag cgcaaccacc gattgcggca gatagcccgc
    11761 gacttcggaa agtggcgtcg gcgtctggaa ctgatgcatc ccaagcagct ccccaccacc
    11821 cgtcgccaaa aatggcagcc gcgccgccag cacccgatac aaaatgatcc ccagggagta
    11881 gacgtcggcg cgatccgtca catcgacgcc gcctttgcac tgctccggcg acatatacgc
    11941 cggcgttccc aaaagtgagt cccctttggt cagattcttg cgcagattcg gatcgcgcag
    12001 cttggcaatg ccaaagtcga gcagcttgac ccgttcccca ccgggaaccg ccggatcacg
    12061 caccagcat
//
LOCUS       DAORGN010000154        12035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2215_length_12035_cov_5.689065, whole genome shotgun sequence.
ACCESSION   DAORGN010000154 DAORGN010000000
VERSION     DAORGN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2215_length_12035_cov_5.689065"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKL17_15530"
     CDS             complement(<1..315)
                     /locus_tag="PKL17_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK46196.1"
                     /translation="MQAPRSTSKGPLWLRALPFVWAALLAMAMASLLARLYGQDPLGM
                     YRLLVAGTWGSVYGIGQVLFKTTPLVCTGLAVAVALRAGLFNVGAEGQLIVGALAAAL
                     CGA"
     gene            complement(322..3459)
                     /locus_tag="PKL17_15535"
     CDS             complement(322..3459)
                     /locus_tag="PKL17_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNK46197.1"
                     /translation="MSRSRLSRLCSLLLALWATLAFVNSASAQKAAIVTATRSAESAW
                     WQYVKQKIVATGLYTAVDQIDAAFRDPTLAQLQDYKLVVVLSSDYGLSSADGVGNALG
                     DYMAMVPGAAVLVFQPNTWQTGLFGAPAIGGKFLTNYALTSQSATAATTPTKRGSVLS
                     NDPLVEGVTEFSCGTNCQRVTGTALRAGATAVAYWADGTPLAIRGKRRVDLNMLPGDE
                     GAISGSWGGSGGELITNAILYLSAPMLQSPRKPTFPSVGLGGASAWTTITFRNISDAP
                     IDITGIGIDGSGKAQFIYRSDYVPTTGSPITLAIGASINVGVAFKPQVQGVHRATLYL
                     AMNGLPRIETALEGTSKGNLWVSQSPIDFGGMPTGMSSSVVTIRLKNAGTMPIDLQKP
                     TIGDSKHYVLTPARPDSSITMFAGATYSFDVQFTPGMDTGEFPTDITILSTDASSPLA
                     IPVRALAGPPKAKVPYSSVLMPDVPMGSKGAPITINLTNEGYSDLSVPSISTDSSDFS
                     VPNAPSAASPLVVPAKETKTFQIVFSPTKAGTRTGTLTLTSNEPPGSPASDKTIALAG
                     TATQPQFKVDVSSLDFGTVNIGASTATKPVELSNAGDGDLLVKEVAIVAGMGADSFAV
                     STLETPPFILRAGSKTTAQVAVGAKSAGMLAATLRIVTDLATGGSATVALTAKANGAV
                     GQIDSAKLDFGDAKVKQLVSKTIKLNNTGNQDLQIIKSALSPTVSVFSLNAPADGTKI
                     PAGGSASFTINCIPSMVGLATARLDITTDDPATPGGTKFSVPLSVNGVVANVTVDPLV
                     LDFTTPIYVGQKSEMKSFKVQNVGSVTVDNLAVKLSGDGAADFTVVTGYKTKILPGES
                     SEIGLTFDPRTAKAEIKAVAVVEADGVQVPMNVQLKGTSMSPLLTVSPTTLRFRGVFV
                     GEQSQSMPITIANDGNSPLEIDVIPPTGDEWIVDLSGVKLLLGPGDSTKVPVVFAPKS
                     VGSKSELIDIRMKGAPVSLAQVSAEGDGLNRPPPAMESSGCAMSSQSTTLPTLVIVAA
                     AAALLLRRRRREGSL"
     gene            complement(3564..4892)
                     /locus_tag="PKL17_15540"
     CDS             complement(3564..4892)
                     /locus_tag="PKL17_15540"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNK46198.1"
                     /translation="MKRLFATAPLAVLLVARAATANPLDAFGYGARAAALGSAYGAVA
                     DDSAANYYNPAGLARSSRLRIDLGYQIARPLVNVSGQRQPLLDTRGLTAGVLLPGSLA
                     GVRFAFGVGLFLPDQHVTRIRVLSFDQPRLHMYDNRTQRLYMAANLAIHIYRGLYVGG
                     GLSFMSRSSGTVFLRGRVGISDPERSELTSSVAQDLFAIRYPQAGLLWEVSKHLTLGL
                     TYRHRFALDLEQGFQIEAQVADPGQRPVVDKGTLKELARSVDLFQPWQLVGAAAARFS
                     RLQVSFDLTFARFSEQPVPAAEFALDLDIGQLTDLVKLPKSKAYPSVGFHDLLIPAVG
                     LEWRALDGMGPLSMDARAGYRYEASPVPDQVGEESLGDADKHIFSLGLGAELVGLTKV
                     LPRPLNLDAYVGLTYLPSRLTQKADPRSAVGDFSVAGFVVQGGLTARFTF"
     gene            complement(4889..6103)
                     /locus_tag="PKL17_15545"
     CDS             complement(4889..6103)
                     /locus_tag="PKL17_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46199.1"
                     /translation="MTGTWRVATLFWLTLSARVSQASLPDMLSLGPRSSALAGTGVSV
                     ANDWEATYQNPAGLGQTQTKLSIGFVYGGYRLRIDDATHELSNPSGLQIGASVPLPLG
                     GMLQDRVGIGLGLYLPSGVVNRVNVPYPEVPRAALFDARTEVVSVLVGIGLRLPWGLR
                     IGGGVLALAALVGDIIIRPDGTGRITTISEEQLTVDYAPIVGARWAPSSERFQLGLTF
                     RGASRSSYLLRLHTELGDAIPLGLPLISFAGIAQYDPLQLAAETTVRLTQNLQIIGQL
                     AWKKWSDYRYPIEPATADSPPLPKPGFHDTIVPRVAVEFQAPTPPFIRLTLRGGYFFE
                     WTPTPAQPPDDDGKSLLDASRHVLCIGLGLSLTGKVPLDVDLFGQAHLLQPGNRLSGQ
                     LFVTGATLGVRL"
     gene            complement(6100..6591)
                     /gene="dtd"
                     /locus_tag="PKL17_15550"
     CDS             complement(6100..6591)
                     /gene="dtd"
                     /locus_tag="PKL17_15550"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNK46200.1"
                     /translation="MRAVVQRVSRAEVRDQETGEVLGKIGVGLCVLLGIGHGDGKKEV
                     DYLVDRITGMRLFPDPPGSVPEGRKPGNINRSLRDVSGSLLVVSQFTLFADTSKGRRP
                     SFLGAMAPDAAIPLYEHFIASCRAAGFPVETGRFGAMMDVDICNDGPVTILLDTSSPP
                     SAS"
     gene            6671..7351
                     /locus_tag="PKL17_15555"
     CDS             6671..7351
                     /locus_tag="PKL17_15555"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNK46201.1"
                     /translation="MQRSNRFSQLTATIAFVATLGTWLPAADADLWSYEHDGVVYFSN
                     IPPSGKDGHRWSVVSRSGPGKAQTVSGVSGCDKSRADVVPSQDTSLDRFHRYDRHIAE
                     ASTVYGLPEALIRAVIKVESDYDPRVISCAGAKGLMQLMPDVATEQKVSDVFEPRKNI
                     LGGSRLLRLLANRFRGDLVLTIAGYHAGAGAVQKYDGVPPYETTQQYVRMVLTEYQRE
                     RARIGGKK"
     gene            7348..8535
                     /locus_tag="PKL17_15560"
     CDS             7348..8535
                     /locus_tag="PKL17_15560"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46202.1"
                     /translation="MSGFLRRFSSAYRTAVAHEAAGEYIEAAKAYALCDERHKVAEMH
                     ILEAGRRAVPGSAAAELLVAVHLLIDDGDAPKSLLKRLGDALLRVVRSRDLVDSDREL
                     LVYAARVLRQADEPSLAAEALERAGDIEQAVVLWQQAGEVERVEKLLLEQAQKRQQAQ
                     REHELFASYETALRLGQRDKAQQSLRALVDVARERREPQRLLDELSRRIPQTGAIELQ
                     LTSRMDEGSFMTERLLCAQVPLRIGRGEDCRLALLDPGVSRLHTQISHSAELGFVLAD
                     LGSKNGTFLDGIRVSEGATLPLRQQGELGIGQHVQLQFTVIGGVLSLLVKSGLRRGLA
                     LRCAPGCLELAKGLQLQFVSGRPVLTAEEGDLRLSGQRVPSRVELIHGDVIERGPLRI
                     EVL"
     gene            8538..9974
                     /locus_tag="PKL17_15565"
     CDS             8538..9974
                     /locus_tag="PKL17_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46203.1"
                     /translation="MGVLERIRSLFLRPRAAAGPVLPSPNAADDPLLLVLRYSQDLGY
                     LTQRPQRVEPSVLRALSALVDSGREELAIQHGLGLCRAIPTDLQLVQFVAETLCRASR
                     YADALWPLEQALRLAPVDNQLRLLSLLATAHESLGDLESARRRLGDLLVLDVHYPEAR
                     RKLLALRPSASLPHRTGETAMAAAVQRAVLPTVAMVTQSRYELRAELGTGRTGTVYRA
                     LDRELGFDVALKVFHPHLRQKDCDDALLRALHEARLLSAARHPGIVALYHVEGDELAG
                     PGQLPLLAMELCRGGSLRTRLRSGPLAVDVALERSIELFDTLAELHAGGVAHGDVKPE
                     NLLFRGDGRGRYLLADSERSLGDLVIADFGLSRLLTGPGGVGVGTFGYLAWERYLGGA
                     PSPLADVFSCAVVLLEMLGCGALPVGNPPKPTPDAGAQFLAAADLGRLGEREAPLRAI
                     AADLLTSTESHRPSAAEAAQRLRCLVRL"
     gene            complement(9901..10938)
                     /locus_tag="PKL17_15570"
     CDS             complement(9901..10938)
                     /locus_tag="PKL17_15570"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46204.1"
                     /translation="MSRGFWLSALCLVSHSALATPPAATEGTPRSVVRDCQLSLWGCS
                     GPAGGSTSGPYDQVIAKARSQIQAGQIEPGKNVASTIAELVEATRQRPDRHEGLALLA
                     MYYAETAACSDGMPLFSRLLAFDDPPLGAAPPSPVLSWRERQTIQLGLALCLSRRGDH
                     AQAVSLYQRLLLGDGPSQRVLYRLGDALLAQGLTDDAVRAYRQACLDVPPLPPLWNLA
                     RSCVGLLVALDRSHQRAPSRVFAQLRRQDPMARYLKMSDFLWPAEPDYYRALTVPAGC
                     ERESRLLRYLQAADATVPRAYRQRAEEHLATTRAQHPGCNVTTGTATNGPGSGAAVPP
                     LPPTDDVIPSK"
     gene            complement(10935..>12035)
                     /locus_tag="PKL17_15575"
     CDS             complement(10935..>12035)
                     /locus_tag="PKL17_15575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46205.1"
                     /translation="VEARVWLGEWLSRPELGPTALVQAVKELQRARQDDDAGSWDFEI
                     ATQLGIVLSHMGRFTEAVTEYDRALRLLPGEPDSLLFPSRHQQATLLSNSAEALMAMG
                     KLEQAIRRYHQAEQIDTGDQGALHAMGLAIAYDRDSQVQKSREALSRSLAADPGLRIY
                     QGDEVFFVPDGDRHYYDGLIAEALGNRDEALRAFRQFVSELPKSRYATRAREHIEELQ
                     KLPGLSAQELLRANVLVGSPHFAPEDSSGGGEKHRSEDEVRRAVQERLIDLRQCYAQG
                     LRRAPRLFGDALVALIVDPSGAVLLVQPLDNNLTERGSYKPSAGQTSAMPPSADLLRC
                     VQSALQRFRFPPAAVGNDDNDELALPLHFESK"
BASE COUNT         2229 a         3642 c         3887 g         2277 t
ORIGIN      
        1 ggcgccacac agcgccgccg caagtgcacc gacgattaac tgaccttctg caccgacgtt
       61 aaaaagtccc gcgcgcagcg ccacggcgac ggccaaaccc gtacagacca gcggagtggt
      121 cttaaacaga acctgaccga tgccgtagac gctgccccac gtaccggcga cgagcagccg
      181 atacatcccc agcggatctt gcccatacag ccgcgccagc agcgatgcca ttgccatggc
      241 cagaagcgcc gcccagacaa acggcagcgc acgcagccac agcggtccct tgctcgttga
      301 gcgtggcgct tgcatgggct gctacagcga cccctcacga cgacggcggc gcaaaagcag
      361 cgcggcagcg gcggcgacga tgacaagcgt cggcagcgtc gtgctctgcg aggacatcgc
      421 gcagccgctc gattccatgg caggtggcgg acggttcagg ccatcgccct cggcgctcac
      481 ttgcgcaagg ctcacgggcg cgcctttcat gcggatgtcg atgagctcgg acttgctgcc
      541 caccgatttg ggcgcaaaca ccaccggaac cttcgtcgag tcgcccggac caagcagaag
      601 cttcacaccc gacagatcga caatccactc atcacctgtc ggcgggatca cgtcgatttc
      661 gagcggcgag ttgccgtcgt tggcaatcgt gatcggcatc gactgagact gctcgccaac
      721 gaatacgcca cggaaacgca gcgtcgtcgg tgacaccgta aggagtggcg acatcgatgt
      781 gcctttcagt tgcacattca tcggcacttg caccccgtca gcctcgacca cggccacggc
      841 cttgatttcg gctttggcgg ttcttggatc gaacgtcagg ccgatttcac tgctttcgcc
      901 gggcaggatc ttggtcttgt aaccggtcac gaccgtgaag tccgcggcgc catcgcccga
      961 cagcttcacc gccaggttgt cgacggtcac ggagccgaca ttttggactt tgaagctctt
     1021 catttcgctc ttctgtccga cgtaaatcgg cgtcgtgaaa tcgagcacca gtggatcgac
     1081 ggtgacgttg gcgaccacgc catttaccga cagtggcacc gaaaacttgg ttccgcccgg
     1141 agtcgccgga tcgtcggtgg tgatatcaag ccgtgccgta gccagaccga ccatcgacgg
     1201 aatgcagttg atggtgaagc tcgcgctgcc accggctggg atcttggtgc catcggcggg
     1261 ggcattcagg gagaacacgc tgaccgtcgg tgacaaggcg ctcttgatga tttgcagatc
     1321 ctggttgccg gtgttgttca gcttgatcgt tttggacacc agctgcttga ccttggcatc
     1381 gccgaaatcg agcttggcgc tgtcaatctg accgacggca ccattggctt tcgcggtcag
     1441 cgcgacggtt gcggaaccgc cagtcgccag atccgtcaca attcgcagcg tcgccgcgag
     1501 catgccagcg gatttcgctc cgacggcgac ttgcgcggtg gtcttgctgc cggcgcgcag
     1561 aatgaacggc ggcgtttcca gcgtcgagac cgcaaacgaa tcggcaccca tgccagcgac
     1621 aatggcaacc tctttgacca atagatcgcc gtcacccgca ttcgatagct cgacgggctt
     1681 ggtcgctgtc gatgcgccaa tgtttaccgt gccaaagtcg agcgacgaca catccacttt
     1741 gaactgcggc tgggtcgccg ttcccgccag cgcgatcgtc ttgtccgacg cgggcgatcc
     1801 cggcggttcg ttgctggtca gtgtcagcgt tccggtccgc gtgccagcct tcgtcggtga
     1861 aaacacgatc tggaacgtct tggtttcttt ggcgggaacc acaagcgggc tcgctgccga
     1921 cggggcgttt ggcaccgaaa aatcggagct gtcggtgctg atcgacggaa cgctcaggtc
     1981 cgaatagccc tcattggtga ggtttatggt gatcggcgcg cctttgctac ccatcggcac
     2041 gtcgggcatc agcaccgagg aatacggcac cttggctttc ggcggacccg cgagcgcgcg
     2101 caccggaatt gccagtggac tgcttgcatc cgtcgaaaga atcgtgatgt ccgtcggaaa
     2161 ttcgcccgta tccattcccg gcgtgaactg gacatcaaag ctgtaggtcg cgcccgcgaa
     2221 catcgtgatg ctcgagtcag gccgcgccgg ggtcagcaca tagtgcttgc tgtcgccaat
     2281 cgtcggtttt tgcagatcga tgggcatcgt cccggcattt ttcagccgaa tcgtcaccac
     2341 actgctgctc attcccgtcg gcatgccgcc aaaatcgatc ggcgactgcg acacccacag
     2401 gttgcctttg ctggtgcctt ccagcgccgt ttcgattcgc ggcaagccgt tcatcgccag
     2461 atacagcgtc gcgcggtgga cgccttggac ctgcggcttg aatgcgacgc cgacgtttat
     2521 cgacgcaccg attgccagcg tgatgggcga tccggtcgtc ggtacatagt cgctgcgata
     2581 aatgaactgg gctttgccgc tgccgtcgat gccgatgccg gtgatgtcga tcggcgcgtc
     2641 ggatatgttg cggaacgtga tcgtcgtcca tgcgcttgcg ccaccgaggc cgacgctggg
     2701 gaaggtcggt ttgcgcgggc tttgcagcat cggcgccgac agatagagaa tcgcattggt
     2761 gataagttcg ccgccgctgc cgccccacga accgctgatc gcgccttcgt caccgggtag
     2821 catgttcagg tcgacgcggc gcttgcctcg aatggcgagc ggcgtgccat cggcccagta
     2881 ggccacagcg gtggctccgg cgcgaagcgc tgtgccggtg acgcgctgac agttggtgcc
     2941 gcagctaaat tcagtcacac cttcgacgag cggatcgttg cttagaacgc tgcctcgctt
     3001 ggttggtgtc gtcgctgcgg tcgcgctctg tgacgtcagg gcatagtttg tcaaaaactt
     3061 gccaccaatc gccggtgcgc caaaaagtcc cgtctgccag gtgttgggct ggaacaccag
     3121 caccgccgca cccggcacca ttgccatgta gtccccgagc gcgttgccga cgccatcggc
     3181 gctcgaaagt ccgtagtcgc tcgatagcac gacgacgagc ttgtagtcct gaagctgcgc
     3241 aagcgtcgga tcacgaaacg ccgcgtcgat ttggtcgacg gcagtgtaaa ggccggtggc
     3301 aacgattttc tgcttcacat actgccacca ggcactttcc gccgagcggg tagcggtcac
     3361 gatcgccgct ttctgggcgc tcgccgaatt tacgaatgcc agcgtcgccc acagcgcgag
     3421 cagcaggctg cacagacgcg agagtcgaga tcgagacatc agagggactc cttcgttcgg
     3481 attcgcggtc aaacatcggt agggaagtcg ggggagacct ccgcccacgt aggggtttag
     3541 cacagcttgt aggcgtgtcg ccgctagaac gtaaagcgcg ctgttaaacc gccctgaacc
     3601 acgaacccag cgacggaaaa atcgccaaca gccgatcgcg gatcggcctt ttgggtaagc
     3661 cggctcggca ggtaggtcag cccgacatac gcatcgaggt tgagcgggcg cggcagcact
     3721 ttggtaagcc ccacaagctc cgcgccgaga ccgaggctga agatgtgttt gtcggcgtcg
     3781 cccaggcttt cttcacccac ctgatcgggc accggcgacg cttcgtatcg atagccagcc
     3841 cgcgcatcca tcgacagcgg acccatgccg tcgagcgctc gccattcgag accgaccgct
     3901 ggaatcagca aatcgtggaa tccgacggac ggataggctt tcgacttggg cagcttcacc
     3961 aggtcggtga gctgtccgat gtcgagatcg agcgcaaatt ccgctgctgg cactggctgc
     4021 tcgctaaagc gtgcaaacgt caaatcgaag ctgacttgca gacgggaaaa ccgcgctgct
     4081 gccgccccga cgagctgcca cggctgaaac aaatcgaccg atcgcgccag ctctttcagt
     4141 gtgcctttgt cgacgacggg acgctgaccc ggatcggcga cctgcgcttc gatttggaag
     4201 ccttgctcga ggtcgagcgc aaagcggtgc cgataggtca gccccagcgt caggtgcttt
     4261 gagacttccc acagcagccc cgcctgcgga tagcgaatcg cgaacaggtc ttgtgccact
     4321 gacgatgtca gctcgctgcg ttcggggtca gaaatcccga cgcgaccgcg cagaaacaca
     4381 gttcccgacg atcgcgacat aaacgacaga ccacccccga cgtacagacc gcggtagatg
     4441 tggatcgcca gattggccgc catatacagt cgctgcgtcc ggttgtcgta catgtgcagc
     4501 cgaggctggt caaagctgag cacccggatt ctcgtcacat gttgatctgg caaaaacaga
     4561 ccgacgccaa acgcaaagcg cacgccggca agtgatcctg gcaaaagcac gcccgccgtc
     4621 agcccgcggg tatcgaggag cggctgtctt tgccccgaca cattgacgag cggacgcgcg
     4681 atttggtagc ccaggtcgat ccgcagtcgc gacgatcgcg caagtccggc ggggttgtaa
     4741 tagttcgctg cactgtcatc agcgacggca ccatacgcgg acccgagtgc ggcagcgcgt
     4801 gcgccataac caaaagcgtc cagcgggttt gccgtcgctg cgcgcgcgac cagcaagacg
     4861 gccaatggtg cggtggcaaa aagtcgcttc atagccgcac cccaagggtg gcacccgtca
     4921 caaacagctg tcccgaaagt cgattgccgg gctgtagcag gtgcgcttgt ccaaacagat
     4981 cgacgtcgag cggtactttg cccgtaagac tcagtcccaa accgatgcac aggacgtggc
     5041 gacttgcatc gagcaggctc ttgccatcat cgtcgggcgg ctgggctggc gtcggcgtcc
     5101 attcgaagaa atagccaccg cggagtgtca ggcgaatgaa tggcggcgtc ggcgcttgga
     5161 attcgacggc aacgcgcggc acgatggtgt cgtggaagcc gggcttgggc agtggcggag
     5221 agtctgccgt cgctggctca atcggatagc gatagtcgct ccactttttc caggcaagtt
     5281 gaccgatgat ttgcaggttc tgcgtcaggc gaacggtcgt ctccgccgct agctggagcg
     5341 gatcatactg ggcaattccg gcaaacgaga tgagcggtag cccaagcgga attgcgtcgc
     5401 ccagctcggt gtgcagccgc aacagatacg acgagcgtga ggcaccgcga aacgtcagcc
     5461 caagctgaaa ccgttcactc gaaggcgccc agcgtgcacc cacgatcggg gcgtagtcga
     5521 cggtgagctg ctcttcggaa atcgtcgtaa tccggcctgt tccgtcggga cggatgatga
     5581 tgtcgccgac cagtgcggcc agcgccagca cgccaccgcc tatgcgcaga ccccacggca
     5641 gtcgcagacc gattccgacc agcaccgata cgacttcggt gcgggcgtcg aaaagggcgg
     5701 cccgtgggac ttcggggtag ggcacattga cgcggttgac gacgccggac ggcagataca
     5761 aaccaagccc gatgccgacg cggtcctgca acatgccgcc aagggggagc gggacgctgg
     5821 cgccgatttg cagaccggac ggattgctca gttcatgcgt cgcatcgtcg atgcgcagtc
     5881 gatagccgcc gtagacgaac ccgatcgaca gcttggtttg ggtctgccca agtcccgccg
     5941 gattttgata ggtggcttcc cagtcgttgg cgacggaaac cccggttcca gccagcgcgc
     6001 tgctgcgtgg gcccaaagac agcatatcgg gcagcgaggc ctgtgacacc cgggccgaaa
     6061 gtgtcagcca aaaaagggtc gcgacgcgcc aggttcccgt catgaggcac tcggtggact
     6121 gctggtatcg agcaaaatgg tgacggggcc gtcgttgcag atgtcgacgt ccatcatcgc
     6181 gccgaagcgc cccgtttcga cgggaaaccc agcggcgcgg cagcttgcga tgaagtgttc
     6241 gtaaagcgga atcgcagcat cgggagccat tgcgccgaga aacgacgggc gccggccttt
     6301 gctggtgtcg gcaaacagcg tgaactgact gacgacgagt agcgaccctg acacatcgcg
     6361 cagcgagcgg ttgatgttgc cgggtttgcg gccttcgggg acgctgcctg gtggatcggg
     6421 aaacaggcgc attccggtga tgcggtcgac gagatagtcg acttcttttt tgccatcacc
     6481 gtgcccgatg ccgagcagca cgcacagccc gacgccaatt ttgccgagca cttcacctgt
     6541 ttcttggtcg cgcacctcgg cgcggctcac tcgctgaaca acggcccgca tcggctggcc
     6601 gtaacacggg gcgaagcttc cgcgcaaggt gacctttttt gtagccgcgg ctgtggctac
     6661 gatgtgtctt atgcagagga gcaatcgatt ttcccagctg acggcgacga tcgcctttgt
     6721 cgcgacgttg ggaacatggc tccctgctgc cgacgcggat ctgtggagct acgagcacga
     6781 cggggttgtc tatttcagca acattcctcc gtcgggaaaa gatgggcaca ggtggtcggt
     6841 ggtttcgcgc agtgggccgg gaaaagcgca gaccgtgtcg ggcgtaagcg gctgtgacaa
     6901 gtcgcgcgcc gacgtggttc cgtcacagga cacaagcctc gatcgttttc accgctacga
     6961 ccgccacatt gcggaagcgt cgacggtgta tgggctgccg gaagcgctga ttcgcgccgt
     7021 gatcaaggtc gagtccgact acgatccgcg ggtgataagc tgcgccgggg ccaaagggct
     7081 tatgcagctt atgcccgatg tggcgacgga acagaaagtc agcgatgtat tcgagccgcg
     7141 caaaaacata ctcggtggat cgcggctgct gcggctgttg gccaatcgct ttcgcggcga
     7201 cttggtgctg accatagcgg gctatcacgc cggggcgggg gcggtgcaaa agtacgacgg
     7261 ggtgccgccc tacgaaacga cgcagcagta cgtgcggatg gtgctgacgg aatatcagcg
     7321 ggaacgggcg cgaatcgggg gcaaaaagtg agcggctttc tgcgccgctt ttcttctgca
     7381 tatcgaaccg ccgtcgctca cgaagcggcg ggtgagtaca tcgaagcggc aaaagcctac
     7441 gcactgtgcg atgagcgaca caaagtcgcc gagatgcaca ttctggaagc agggcgtcgc
     7501 gcggtgccag gaagtgccgc tgcggagctg ttggtcgccg ttcacctgct gatcgacgat
     7561 ggggacgcgc caaaatcgct gctcaagcgg cttggcgatg cgcttttgcg cgtggtgcgc
     7621 agtcgagatt tggtcgacag cgaccgcgaa cttttggtct atgccgcgcg ggtgcttcgc
     7681 caagccgacg agccgagcct ggcggcggaa gcgctggagc gcgccggcga catcgagcaa
     7741 gccgtggtgc tttggcagca agcaggtgag gtcgagcgcg tcgagaagct gcttttggag
     7801 caagcgcaaa aacgccagca ggcgcagcgg gaacacgaac tgtttgcgag ttacgaaacg
     7861 gcgctgcggc tcgggcaacg cgacaaggca cagcagagcc tgcgcgcgct cgtcgatgtg
     7921 gcccgtgaga ggcgtgaacc gcagcggctt ctcgatgagc tgtcacggcg gattccgcaa
     7981 accggcgcaa tagagcttca gctcacgagt cgcatggacg aaggatcgtt catgacggag
     8041 cggctgctgt gtgcgcaggt gccgctgcgg attggtcgag gggaagactg tcggctcgcg
     8101 ctgctagatc ctggggtatc gcgactgcac acgcagataa gccacagcgc agagcttggc
     8161 tttgtgctcg ccgacctggg cagcaaaaac ggcacgtttc ttgacggaat tcgggtgtcg
     8221 gaaggcgcga cactgccgct gcggcagcag ggggagctgg gcatcggtca gcatgtacag
     8281 ttgcaattta ctgtcattgg cggcgtgctg tctttgctcg taaaaagtgg tctgcgtcga
     8341 ggtctcgccc tgcgctgtgc acctggctgc ctggaacttg caaaaggatt gcagcttcag
     8401 tttgtatccg gacggcccgt tttgaccgcg gaagaggggg acctgcggct gtcgggtcag
     8461 cgggttccat cgcgggtaga gctgattcat ggcgacgtga tcgagcgtgg tccgctgcgt
     8521 atcgaggtgc tgtgacgatg ggcgtgcttg agcggattcg gtcgctgttt ttgcgcccgc
     8581 gcgccgctgc tgggccggtg ctgccgtcgc caaatgccgc tgatgatccg ctgctgctgg
     8641 tcctgcgcta cagccaagac ctgggttatt tgacccagcg tccgcagcga gtcgagccgt
     8701 cggtgctcag ggcgctttcg gcgcttgtcg attcgggacg ggaagaactg gcgattcagc
     8761 atgggcttgg gctgtgtcgg gcgataccga cggaccttca gcttgtgcag tttgtcgccg
     8821 agacgctgtg ccgcgcctct cgctatgccg atgcgctgtg gcctttggaa caagcgctgc
     8881 ggcttgcgcc ggttgacaat cagcttcgac ttctttcgct gttggcgacg gcacatgagt
     8941 cgcttggtga tctggaatcg gcgcggcggc gtcttgggga tctgctcgtg ctcgacgtac
     9001 actatccgga agcgcgacgg aagcttttgg cgttgcgtcc gtccgcgtcc ctgccgcatc
     9061 gcactggcga aacagcgatg gctgcggcgg tccagcgagc agtccttccg acggtggcga
     9121 tggtgacaca gagccgctat gagctgcgcg ccgagctggg cactgggcgc accgggaccg
     9181 tctatcgggc gctggaccga gagctgggct tcgacgtggc gctaaaagtc tttcatccgc
     9241 acttgcgaca aaaagactgc gacgacgcgc tcttgcgggc actccatgaa gccaggcttt
     9301 tgtcggcggc gcggcatcct ggcattgtgg cgctttacca cgtcgaaggg gatgaactgg
     9361 ctgggccggg gcagctgccg ctgttggcga tggagctgtg ccggggcgga tcgttgcgaa
     9421 cgcggctgcg aagcgggccg cttgcggtcg acgtggcact ggaaagaagc atcgagctgt
     9481 tcgatacgct tgcggaactg catgccggcg gggtcgctca cggcgatgtg aagccggaaa
     9541 acctgctgtt tcgcggcgac gggcgtggcc ggtatttgct ggcagattca gagcgttcgc
     9601 tgggcgactt ggtgattgcc gattttggtc tctcgcgact tttgaccggg cctggcggcg
     9661 tcggcgtggg aacgtttggc tacctggctt gggaacgcta tcttggcggc gcgccatcgc
     9721 ctttggcgga tgtgttttct tgtgcggttg tcttgctgga aatgctcggc tgtggcgcac
     9781 ttcccgtcgg aaatccaccc aaaccaacac cggatgccgg ggcccagttt ttggcggcgg
     9841 cggacttggg ccggctcggt gagagggaag cgcccctgcg cgccatcgcc gcggatctgc
     9901 ttacttcgac ggaatcacat cgtccgtcgg cggcagaggc ggcacagcgg ctccgctgcc
     9961 tggtccgttt gtagctgtac ccgtcgtcac gttgcagccg ggatgctggg cgcgggtcgt
    10021 ggccagatgt tcttccgctc gctgacggta tgcgcgtgga acggtggcgt cagccgcttg
    10081 cagatagcgc agcagtcgag actctctctc gcagccagcg ggcaccgtca gcgcgcggta
    10141 atagtccggc tccgcgggcc acagaaaatc gctcattttc agataacggg ccatcggatc
    10201 ttggcgtcga agctgggcaa acacgcgact tggggcacgc tggtgacttc gatcgagcgc
    10261 gacgagcagg ccgacacagc ttcgcgcaag attccacagc ggcggcagcg gcggcacgtc
    10321 gagacaggct tgccgatagg ctcgcacggc gtcatcggtc agcccttggg caagcagcgc
    10381 atcacccagt cgatacagca cgcgctggct tggcccgtcc ccaagcagca gtcgctgata
    10441 cagcgaaacc gcctgtgcat ggtcgccccg ccgcgataga cacagggcca gcccgagctg
    10501 gatcgtctgc cgttctcgcc acgacagcac tggggacggc ggcgcggctc ccaacggcgg
    10561 atcgtcgaac gctagaagcc gcgaaaacag tggcatcccg tcgctgcatg ccgcggtttc
    10621 ggcgtaatac atggccagca aggcaagacc ttcgtgtcga tccggtctct gacgggtggc
    10681 ttcgaccagt tcggcaatcg tggatgcgac gttttttccg ggttcgatct ggccggcttg
    10741 gatctggctg cgtgctttgg cgatgacttg gtcgtagggg ccgctcgtcg atccgcctgc
    10801 ggggccgcta cagccccaca gcgatagctg gcagtcgcgc accacacttc gcggcgttcc
    10861 ttcagtcgct gcgggaggag tggcgagcgc gctgtgggat accaaacaga gtgcggaaag
    10921 ccagaaaccg cggctcattt ggactcgaaa tggagaggca aggccagctc gtcgttgtca
    10981 tcgttcccga cggcagcggg cggaaagcgg aaccgctgga gcgccgactg gacacagcgc
    11041 agaagatccg ccgatggtgg catcgccgat gtctgacctg ccgatggctt gtacgatccg
    11101 cgttcggtca agttgttgtc gagcggctgc accagcagca ccgcgccgct cgggtcgaca
    11161 atcagcgcca ccagcgcatc gccaaacagc cggggagccc gacgcagacc ttgggcgtag
    11221 cactggcgaa gatcaatcag ccgctcctgc acggcccgcc gcacttcgtc ttcgctgcgg
    11281 tgtttttcgc cgccaccgct gctgtcttcc ggggcaaagt gtggcgaccc gaccaggacg
    11341 ttggcgcgca gaagctcttg cgccgatagg ccaggcagtt tttgcagctc ctcaatgtgt
    11401 tcacgggccc gtgtcgcata acggctcttg ggcagctccg aaacgaactg acggaatgct
    11461 cgcagcgctt cgtcgcgatt gcccagcgct tccgcgataa gcccgtcgta atagtggcga
    11521 tctccgtcgg gaacaaagaa cacttcatcg ccctggtaga tccgcagccc cggatcggcg
    11581 gccagcgatc gactgagcgc ctcgcggctt ttttgcacct ggctgtcgcg gtcatacgca
    11641 atcgcaagtc ccatcgcatg cagcgcgccc tgatccccgg tgtcgatttg ttccgcttga
    11701 tggtagcgac gaatcgcctg ttccagcttg cccatcgcca tcagtgcttc ggcggaattc
    11761 gacagcagcg tcgcttgctg atgacggctt gggaacagca gcgaatcggg ttcaccgggc
    11821 aaaagccgca gcgcgcgatc gtattccgtc accgcttcgg taaaccggcc catgtgggac
    11881 agcacgatgc caagctgcgt tgcgatctcg aaatcccaac ttcccgcgtc gtcgtcttga
    11941 cgggcgcgct gcagttcctt gaccgcttgc acaagcgccg tcgggccaag ctctggtcgc
    12001 gacagccact cgccaagcca cacccgcgct tcgac
//
LOCUS       DAORGN010000155        11952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2236_length_11952_cov_5.986215, whole genome shotgun sequence.
ACCESSION   DAORGN010000155 DAORGN010000000
VERSION     DAORGN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2236_length_11952_cov_5.986215"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="PKL17_15580"
     CDS             <1..1481
                     /locus_tag="PKL17_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNK46206.1"
                     /translation="RPVGHFLVEESTADGQHQSGSLRHNFAALSTPSPKSPDPKAAKP
                     TQIATLAAAFGPDIAHAWRSAVKFRSFALSALLVAFASPVAHAGDAPTAEQKVWEDKL
                     RRMAPPAPAAAPMGGGALASQSPKASSASFEFSDATVEGSLGTTEMRKANRVAAEILQ
                     RRMEETRPNDFKLIREAAGADVVVLSGVYDRVQDVLRAVQIKHVVVPPHLLDQMELLS
                     TQTVMLNCPGNVTDVGVKKLRDFVARGGYLVSTDWALRTVEKAFPGTISHNGKTTPND
                     VVSVQVHDDKEPLLRHVKVMREHPRWWLEGSSYPIHIKDPKQVRVLMSSQEMQAKYGD
                     GAVVVSFHHQQGKVLHMTSHFFLQQSKLVEGSEKQKGSSFAKGAGLSDAQIGDLTKKG
                     IAVDDVKAGELNAAYSMQQVSTNLIAEKQAQNQEMLRKRYSLRVRPDDLALSSDGTAA
                     PTAQSPRLKKDFRVEVLERKGKQAKVRDLFGHEGWVQSDALY"
     gene            1704..2987
                     /locus_tag="PKL17_15585"
     CDS             1704..2987
                     /locus_tag="PKL17_15585"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNK46207.1"
                     /translation="MSQTRREVLQGLLGLSSVGLSAAATGLPVSLLLSPHTAVAANPA
                     QSSRSRFLVLSLSTCGDPLNANVPGCFAAPDIVHPVDPRFSAAPISIAGQRHQAAQVW
                     KQLPQKLLDRTVFFHHATNKNSHPSLPELLQVGPQAESAPVLFAQALARPLDTVQSQP
                     LVLGADEVLTQADKRLPGIRPTELRDALLGPGSPLPQLAKLRDATLARLSHLKNKTGS
                     PALAQALHTQAMTAKQAQILGEQLAADLAALKTDGAEAQVVAAAIAIRLGISPVIAIH
                     VPFGGDNHFDSGLRQEAEQIHSGIGHVAKLWNKLAAYGVAERSCFAHLSVFGRTLKRE
                     GLLGRDHWPLHNTAIVQGPSFRGGVIGGVVAQDGDYAAQAIDSRTGQQSPGGDILPSD
                     SGQALLRTIGQGIGIDTATLDAQLPQAKSIRAHLV"
     gene            complement(3000..4454)
                     /locus_tag="PKL17_15590"
     CDS             complement(3000..4454)
                     /locus_tag="PKL17_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46208.1"
                     /translation="MMKRCTLLGTVGSRRLALSLTIGLSFANVASAQTSVPKPLLSVK
                     PDKGYIDDAMAISDGGRVFLYVNTDGTKFATLRAVALPPGSGLNPQAAVDAMPPPVVP
                     PPPAQPAKGVKGKPTPPPAPPAVPPPPAAPFMEEWLARELLAGPTGMKELLSGLPLNT
                     SRLHLLSEDRVLVVMRDLETSGLYTASIYSLKTRAEVPVPGGIGPATDITLTMIDGQK
                     AILAVQKPGPQTPDNPRSLLSSEFKFSAFSVATGKPIGQKTYKLDEEGRISTAAGLAL
                     PLYFLDGYATWAVKQAGAFDKKKDLRQPDHLAFLDVMSGKVRATRPITDPPALMDLKR
                     VRASHSSSVAIIAEDGTNQIEIVAALARGLPEGPVESRGQLPLPRPAGIYDSPTVRYA
                     SLRPTTLLLSLMVDPVNEEAVAAKRTDPDEIDFCTVDLSGDKPGPAQRKLTLLAGKRP
                     FGWVVSPSGRLVFLRKHKGFSRGGTEAEVYDLAL"
     gene            4540..9501
                     /locus_tag="PKL17_15595"
     CDS             4540..9501
                     /locus_tag="PKL17_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46209.1"
                     /translation="MAKKHSSAQKQHAIEGSDSAHHDEPQLAPGEHSQDGLSLLMQRL
                     RAGRVVLCTGSIFARSTETSSFRSLLAKLLSQSGSSLGNEEAAQARALLDSQPLLVAS
                     LLRKQLGDKLPQAIADVLPKEANTELFGLAARLPFRGIITTALDSGLLDAVQNAPAPG
                     GAPQIGTSEQTIELLASRGRFMLRLCGDAQSGQGLIFGEADVQKIFSSEALRQSLRDL
                     ALKRSLLLTGFDVHDPDLAMTARLLSLAQTTGEGPQHFALLPGLPAGLREELSRAYGL
                     IVLDGSDEEALFRTLQQSVGEIGSEILPDDDDLEGWLRILQQEPDHQDARAKLSALED
                     KLRSQGDHDRLIELLLGRMDVEPQSSGRASCLIQVAQLFETAKGQLAEAFQAALAAYK
                     EDPKTVSLDEVERLAGQAGQWVELLTEMRDLVPRQPRGEQAVLWLRIARLYGEKLNHH
                     EYALASLGEAQKLGIPDGEKKALLLLRTDLSRRAQRWKDLADALGQLAELCEEKDKCV
                     DCYMEQGELLETRLSDGAAAIDAYKKARQADPNSRDALVALEHTLRRAARWDELIVLL
                     DEKAGLAAAQSDAPGELKARREAAQIQAEHGTDRKASIRRWEDVHKLAPEDIDVLRAL
                     ERLYANEGSVSEAYLKVLSDLAQLVPSDKERLTLYRRLAAEYEEMPGQLDLATAALEK
                     VLDIDATAEDAYRSLCRLYRQQKKWPELCATYERHLAHAEKGRGELLTALSRVYEVDI
                     PAGDEQRRREEAPKAIAAWQKLLDMEPEHAGAIEALGRLYQLTEQPAEAVRLLVKRAH
                     LTDDKNLKANLYNEAARLCLRKLDDSSSAEEHLVRALEILPGHVASQTALADLYRTRG
                     DHLRAAKLLAEASESSQNRLDKGRFVVQAAKELLLGEDRQKAADLLREVLAQDPEQSD
                     AAQLMSELYWQDGRFDDALPLLEMLTRKEAERGIQVVRLVRLSQACLGAGLRDKARRA
                     YRRALELDGKDLGALRGLLPLLIETGQFVDARQLSETLLAEHRESLSRPELAELFATL
                     GQAEMALGNPDAALSALQKAREQEPMHRRALSQLRRIDQLAAEEKLEVRQSLLRSLLS
                     QSGEPGTTDERIAVLTEIGDLHAGPLANPADALSSYREALSLSPKAVTIQHKLLSLYT
                     EQQMWPEAADLLQELAAAETNEKRRARYKQTAGFITRDHLKEPRRALRLLWSSFDDDP
                     SLVKSLESAEALAAQIGDPKELLRAMQRRIKVMGSDAQDSPKQRAERLRLWTEVARVC
                     IQELGDLKTGMAAYEVSLQLDPQNMDRHRQMAAIYASAGDDKLDKAIAEHHLVLKSNK
                     SELASFRALRDLYGQTGQRDRAAQVAYALHLLRQAEPEDDKLVDELKSRPVTLARRPL
                     TKELWRLLAHPEEDARLQSLLAVLWPALQKSHARPWTEQPFARDSRVEIGSSQVYLKA
                     LRYAFEMTEAPLPELFPRPQARELAEQAFLISIASEGKGTATVCAELGPPLLDPSRPE
                     RETLYEVGRMAALLRPERALRTVLTDELELAQILEAALVLTHQELSQTTPPASAQVLE
                     LARSLRGALTAQQLDQLSRIGRSLLDSSVKPQQLASTWISASELTTVRVGLLLSGDLE
                     TAALLLATDPPGLTLLSPKQRLLELLYFAVSEELASVRQFLGL"
     gene            9574..10674
                     /locus_tag="PKL17_15600"
     CDS             9574..10674
                     /locus_tag="PKL17_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46210.1"
                     /translation="MDSDEQNRSARPGRPQLRLVPPEPAESTGEVSGIAPSKSASAAQ
                     IRALPVGPGTPSLRESYVFGAKMLLDQPAACRHGYRLFWVHGRDLGWIDLEASSSVFA
                     VMGWHENCDLVLPKLPNITNRHLLASCALLADKTPALRLLDLHTNQPFTIEGQPRQSI
                     GVSGPLLIGLGDCAIGCVPLPEFLQQRGRLADGVELPSYDIVEAEQPPQHSLRMQRRT
                     HITLLPRSQRVEDLQSGPKVATAPAGRARVTLRRQTKAVSVELSEADLYAGVIIGRSE
                     TCMDRGLRQVLSGQISRLHVMLLAERGRVYAMDLCSTNGTWQHGQRVRRVELPDQGTQ
                     LLLSGNDSKVELTWHPRQPNIRSTRAEGPAGD"
     gene            10690..11268
                     /locus_tag="PKL17_15605"
     CDS             10690..11268
                     /locus_tag="PKL17_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNK46211.1"
                     /translation="MTTYSSTLDRALARSATAHRNQARKGSDIPYIIHPVHVAMILLR
                     HDFPEEVIVAAILHDVVEDTGTTLEELAAEFGPEVARLVGAVSEQKSEGAQPLPWRVR
                     KEAQLEHLRHADRHTAALKTADALHNLRATLVDLKNQGDAVWTRFRGSRTDHLWYYTS
                     LSEILRPILGEHPLSVELSHAIRELRETQKLT"
     gene            complement(11269..>11952)
                     /locus_tag="PKL17_15610"
     CDS             complement(11269..>11952)
                     /locus_tag="PKL17_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK46212.1"
                     /translation="IIRWHIKSTTKAQHGLYEYDAVARLRDSQLGDPRVHDIAHYIVR
                     GKLWEAFAADSAPVLLIDEIDKADIEFPNDLLTELDRMEFYVYETKQTIAAKNRPIVI
                     ITSNNEKELPDAFLRRCFFHYIRFPDAETMTKIVSVHYPLLKSSLLREALSVFYELRE
                     VPGLKKRPSTSELLDWIKLLVCEDLPPEALRSRDPGKILPPLIGALLKSEQDHFLVER
                     MASHVRRGR"
BASE COUNT         2419 a         3749 c         3662 g         2122 t
ORIGIN      
        1 cccggccagt cggtcacttt ctcgtcgagg agtccaccgc cgatggtcag catcagtcgg
       61 gaagtttacg ccacaacttt gccgccctgt caacgccatc ccccaagtcg ccagacccga
      121 aggcagcaaa acccacgcag atcgccacgc tggctgctgc gtttgggccg gacatcgccc
      181 acgcttggag gtccgctgtg aagttccgat ccttcgccct gtccgccctg cttgtcgctt
      241 ttgcgtcccc cgtcgcccac gccggagacg cccccaccgc cgagcaaaaa gtctgggaag
      301 acaagctccg gcggatggcc ccgcccgcac ccgctgcggc cccgatgggc ggcggcgcac
      361 tggccagcca gtcccccaag gcgtcatccg ctagcttcga gttttccgat gccaccgtcg
      421 aaggttcgct tggcaccacc gagatgcgca aagccaaccg cgtcgctgcc gaaatcctcc
      481 agcgccgcat ggaagaaacc cggccaaacg acttcaagct gattcgcgag gcagcgggtg
      541 ccgacgtggt ggtgctttcc ggcgtctacg atcgagtgca ggacgtgctg cgggcggtgc
      601 agatcaaaca cgttgtcgtg ccacctcacc tgcttgatca gatggagctt ttgtcgacgc
      661 aaacggtgat gctcaactgc cccggcaacg tcaccgacgt aggggtcaaa aaactgcggg
      721 acttcgtcgc ccgcggcggc tatttggttt caaccgattg ggcgttacgc acggtcgaaa
      781 aagcctttcc cggcaccatt tcccacaacg gaaagaccac gccaaatgat gtggtttccg
      841 tgcaagttca cgacgacaaa gagccgcttt tgcgccacgt caaagtgatg cgcgaacatc
      901 cccgctggtg gctcgaagga tcgtcgtatc ccatccacat caaagacccc aaacaggtgc
      961 gggttctgat gtcgtctcaa gaaatgcagg ccaagtacgg cgacggcgca gtcgtggttt
     1021 cgtttcatca ccagcaaggc aaagtgctgc acatgaccag ccattttttc ctgcagcaat
     1081 cgaagctggt cgaaggcagt gaaaaacaaa aaggctcaag ctttgccaaa ggcgcaggtc
     1141 tttccgacgc gcagattggc gatctgacga aaaaaggcat cgccgtcgac gatgtcaaag
     1201 cgggcgagct gaatgcggcg tactcgatgc agcaggtatc gaccaacctg attgcggaaa
     1261 agcaggcgca gaaccaagaa atgctccgca agcgctacag cctgcgggta cgcccagatg
     1321 atctcgcgct cagcagcgac ggaacggcag caccgacggc acaaagtccg cggctcaaaa
     1381 aagacttccg cgtcgaagtg ctggagcgca aaggcaagca agccaaggtt cgcgacctgt
     1441 ttgggcatga aggctgggtg caatccgacg cgctgtactg atcctcctcc agacggcgga
     1501 ccgatacctt cacagggtta cacgctcgcc ggaaacaatg acgatttgcg cgtcatcggt
     1561 cgtcagcttt ggggcgggtg gcgataaaat ggaaccttct ggattgagcg attcgcttgt
     1621 ccgccgtccg ccagcgtatg cgtgagaagt tgagaccaaa tccgctcctt cgtacaagct
     1681 gacgaagaag caggagggcc cagatgagcc agacgagacg cgaagtgctg caaggtctac
     1741 tcggattaag ctcggtgggc ttatcggcag cagccacggg attgccggtg agcctgctgc
     1801 tttcgcccca caccgccgtc gcagcaaatc cagcccagtc ttcgcgaagt cgttttttgg
     1861 tgctcagtct gtcgacgtgc ggcgatccgc tcaacgccaa cgtccccggc tgtttcgccg
     1921 cgcccgacat cgttcatccc gtcgatccac gcttttctgc cgcgccgatt tcgatcgcgg
     1981 gtcagcgcca ccaagccgcg caggtctgga aacagctgcc gcaaaagctg ctcgaccgga
     2041 cggtgttttt ccatcacgcg acgaacaaaa acagccatcc cagcctgcct gagctcttgc
     2101 aagtcgggcc gcaagccgag agcgcgccgg tgctgtttgc gcaggcgctc gcacggccac
     2161 tcgatacggt gcaaagtcag ccgcttgtgc tcggtgccga cgaagtactg acgcaagccg
     2221 acaagcgatt gccgggaatt cgaccgacag agctgcgcga tgcactgctc ggaccgggat
     2281 cgccgctgcc ccagcttgcg aagctccgcg atgccacgtt ggcccggctg tcacatctga
     2341 aaaacaagac cggcagcccg gcgctcgcac aagcgctgca cacccaagcg atgacggcga
     2401 agcaggccca gatcctcggt gagcagcttg cagccgatct ggcggcgctg aagaccgacg
     2461 gggcggaagc ccaggttgtt gccgctgcga ttgccatccg gctcggcatt tcgccggtca
     2521 tcgcgatcca cgttcccttt ggcggagaca accacttcga cagcggactg cgccaagaag
     2581 ccgagcaaat ccacagcggc atcggacacg tcgccaagct gtggaacaag ctggccgctt
     2641 acggcgtcgc tgagcgaagc tgttttgccc atctttcggt gtttggccgc acgctcaagc
     2701 gcgaaggact cttgggacgc gatcactggc cgctgcacaa cacggcgatc gttcagggtc
     2761 catcgttccg cggcggtgtc ataggtggtg tcgtcgccca agacggtgac tatgccgccc
     2821 aggccatcga ctcgcgcaca ggtcagcaaa gccccggcgg cgacattttg ccaagcgaca
     2881 gcggtcaagc gttgctgcgc acgatcggcc aaggcatcgg catcgacact gccacgctcg
     2941 acgcgcagct gccgcaggcc aagtcaatcc gcgcccactt ggtctaaccg cagcgtttct
     3001 tacagcgcca aatcgtagac ttcggcttcg gtgccgccgc gcgaaaagcc tttgtgcttg
     3061 cgcaaaaaca ccaaacgtcc cgacggtgaa accacccaac cgaacggcct tttgcccgcc
     3121 aaaagcgtca gcttacgctg cgctggcccc ggcttgtccc ctgacaaatc gacggtgcaa
     3181 aagtcgattt catcgggatc ggtgcgcttg gccgcgaccg cttcttcatt gaccggatcg
     3241 accatcagcg acaaaagcag cgtcgtcgga cgcaaactgg cataacgcac cgtcggagaa
     3301 tcgtagatgc ccgccggacg cggcagtgga agttgaccgc gtgactcgac gggaccttca
     3361 ggcagaccgc gcgccagtgc ggccacgatt tcgatctgat ttgtgccatc ttcggcaatg
     3421 atcgccaccg acgaggaatg cgaagcccga acccgcttca aatccatcag ggccggcggg
     3481 tcggtgatcg gtcgcgtcgc ccggactttg ccgctcatca cgtcgagaaa cgccaaatgg
     3541 tcaggctgcc gaagatcctt ttttttgtcg aaagcgccgg cctgcttgac cgcccacgtt
     3601 gcatagccat cgaggaaata cagcggcagc gccagccccg ccgccgtcga aatccgtccc
     3661 tcttcatcga gcttgtaggt cttttggccg atcggtttgc cggtcgcgac cgaaaaagcc
     3721 gaaaacttga actcgctgct gagcaagctg cgcggattgt ccggcgtctg tggccccggt
     3781 ttttgcaccg ccaagatcgc cttctgtcca tcgatcatcg tcagggtgat gtccgtcgct
     3841 gggccaatcc cgcctggcac cggaacttcg gcgcgggtct tcaggctgta aatgctcgcg
     3901 gtgtaaagcc ccgatgtctc gagatcgcgc atcaccacca gcacgcgatc ttccgacaaa
     3961 agatgcagcc ggctggtatt gagcggcagc cccgacaaaa gctctttcat acccgtcggc
     4021 cccgcaagca gctcgcgcgc cagccactcc tccataaagg gtgccgcagg tggcggtgga
     4081 acggcaggcg gcgctggcgg cggcgtcggc ttgcctttga cgcccttggc tggttgcgct
     4141 ggcggcggcg gaacgacggg cggcggcatc gcatccactg cggcctgcgg attgagccca
     4201 cttcccggcg gcagcgcgac cgctcgcagc gtcgcaaact tggtcccgtc ggtgttgaca
     4261 tacagaaata cccgcccgcc gtcggaaatc gccatcgcgt cgtcgatgta gcccttgtcg
     4321 ggcttgaccg agagcagcgg ctttggcaca gaagtctgcg cagaagcgac gtttgcaaac
     4381 gacagtccga tcgtcagcga caacgccaag cgacgcgaac ccaccgtacc tagcagcgtg
     4441 catcgtttca tcatgattta gccgtatggc atggactgtc ggctgtcaac ctgaacagtg
     4501 ttgaacggcg tcggcgtgct gtgttaaccc aaggccgaaa tggccaaaaa gcactcttct
     4561 gctcaaaaac aacacgcgat cgagggctct gactcggcgc accacgacga accgcagctt
     4621 gctcccggcg agcactcgca agatggcctg tctctgctta tgcagcgact gcgcgccggt
     4681 cgggtggtgc tatgtaccgg cagcattttt gcccgttcga ccgaaacgtc gtcgttccgc
     4741 agcttgcttg ccaagctcct ttcgcagagc ggtagcagcc tgggcaatga agaagcggcg
     4801 caggcccgag cactgcttga tagccagcca ctgctcgtcg ccagcttgct gcgcaaacag
     4861 ctcggggaca agctgccgca ggcgatcgcc gacgtgctgc ccaaagaggc caataccgaa
     4921 ctatttggcc tggcggcgcg gctgccattt cgcggcatca tcacgacggc gctcgacagc
     4981 ggactgcttg atgcggtgca aaacgcgccg gcccccggtg gagcgccgca gatcggtacg
     5041 tcggagcaga cgatcgaact tttggccagt cgcggtcgct tcatgctgcg tctgtgcggt
     5101 gatgcccaga gcggccaggg cctgatcttc ggcgaggccg atgtccaaaa aatcttctcc
     5161 agtgaggcgc tgcgccagtc gctgcgagac ttggcgctca agcgatcgct gctgctaact
     5221 ggtttcgatg ttcacgatcc cgatctggcg atgacggcgc ggcttttgtc gctggcgcag
     5281 acgacgggag aaggcccgca acactttgcc ctgctgcccg gtctgccggc gggtctgcgc
     5341 gaagaactgt cgcgcgccta cggactgatt gtgctcgatg gcagcgacga agaagcgctg
     5401 ttccgcacgc tccagcaatc ggtcggtgaa atcggcagtg aaattctgcc cgacgacgat
     5461 gacttggaag gctggctgcg catcctgcag caagaacccg atcaccaaga tgcgcgggcc
     5521 aagctgtcgg cactcgaaga caagctgcgt tcgcaaggcg atcacgatcg gctgatcgag
     5581 cttttgctcg gtcgcatgga cgtcgagccg cagtcgagcg gtcgtgcgtc gtgccttatc
     5641 caagtggcgc agctgtttga aaccgccaaa ggtcagctgg ccgaagcgtt ccaagccgcg
     5701 ctggccgctt acaaagaaga cccgaagacg gtgagcctcg atgaagtgga gcggctcgcc
     5761 ggtcaggccg gacagtgggt cgagctgctg actgaaatgc gcgatctggt gccgcgacag
     5821 ccgcgggggg aacaggccgt cctgtggctg cggattgccc gtctgtacgg cgaaaagctc
     5881 aaccaccacg aatacgcgct ggcgtcgctt ggggaagccc aaaaacttgg tattcccgac
     5941 ggagaaaaga aagcactgct gctgttgcga accgatctgt caaggcgggc gcagcgctgg
     6001 aaagacctgg ccgatgcgct cggtcagctg gcggaacttt gcgaagaaaa agacaagtgc
     6061 gtcgactgct acatggaaca aggagagctg ctggagaccc ggctttccga tggtgcagcc
     6121 gcgattgatg cgtacaaaaa agcccgccag gccgatccga acagccgcga tgcattggtg
     6181 gcgcttgagc atacgctgcg acgggctgcc cgctgggacg agctgattgt gctgctcgac
     6241 gaaaaagccg gtcttgccgc ggcacaaagc gatgccccag gcgagctgaa agcccgacgc
     6301 gaagcggcac agattcaggc cgaacatggc accgatcgca aagcaagcat tcgtcgctgg
     6361 gaagatgtcc acaagctggc cccggaagac atcgacgtgc tgcgggcgct cgagcgcttg
     6421 tacgcaaacg aaggcagcgt ttcggaagcg tatttgaaag tgctgtcgga tttggcgcag
     6481 cttgtgccaa gcgacaaaga acgactgacg ctgtaccgtc ggctcgctgc tgagtatgag
     6541 gaaatgcccg gtcagctcga tttggcgacg gcagcgctcg aaaaagtgct ggatatcgac
     6601 gccacagccg aagatgcgta ccgatcgcta tgtcggctgt atcggcagca gaaaaagtgg
     6661 ccggagctgt gcgcgacgta tgaacggcac ctggcccacg cggaaaaagg cagaggcgag
     6721 ctgctcacgg cgctttcgcg ggtgtacgaa gtcgacattc cggcgggcga cgagcagcgc
     6781 agacgcgaag aagcgcccaa ggcgattgcg gcgtggcaga agctgctgga tatggaaccc
     6841 gagcacgccg gtgcgatcga ggcgctggga cggctttatc agcttaccga acagcccgca
     6901 gaggccgtcc ggctgctggt caagcgggcc cacctgaccg acgacaaaaa tctcaaggcg
     6961 aacctgtaca acgaagcggc gcggctgtgt ctgcgcaagc tggacgacag cagcagcgcc
     7021 gaagaacacc tggtccgggc gctggaaatt ctgccgggtc atgttgcgtc gcagacggcg
     7081 ctcgcggacc tgtataggac ccgcggcgat cacttgcggg ccgcgaaact gctggccgaa
     7141 gcgtcggaat cgagccaaaa ccggctcgac aaaggtcgtt tcgtggtgca ggccgccaag
     7201 gaactgctgc tcggtgagga tcgccaaaaa gccgcagatt tgctgcgtga agtcctggcc
     7261 caagatcccg agcaaagcga cgccgcgcag cttatgtccg agctgtattg gcaagacggt
     7321 cgcttcgacg atgccttgcc gcttttggaa atgctgacgc gcaaagaagc cgagcgtggc
     7381 attcaagtcg tgcgtctggt gcggctttcg caggcgtgcc tgggtgcggg actgcgcgac
     7441 aaagcacggc gggcgtatcg acgagcgctc gaactggatg gcaaagacct tggcgcactg
     7501 cgcgggctgc tgccgctgct tatcgaaacg ggtcagtttg tcgatgcccg gcagctcagc
     7561 gaaacgcttc tggccgaaca ccgagagtcg ctgagccgac ccgagctggc cgagctgttt
     7621 gcgacgcttg gtcaggcgga aatggcactg ggcaatccag atgcggcgct ttcggcgcta
     7681 caaaaagccc gcgagcaaga gccgatgcat cggcgagcgc tttcacagct ccgtcgcatc
     7741 gaccagctcg cagcggaaga aaaactggag gtgcggcagt cgctgctccg atccctgctg
     7801 agccaatcgg gagaaccggg aaccaccgac gagcgaattg cggtactgac cgaaatcggc
     7861 gatctgcatg ctggcccgct cgccaatccg gcagatgcgc tgtcgtcgta ccgcgaagcg
     7921 ctgtcgctgt cgcccaaggc agtcacgatc cagcacaagc tgttgtcgct ttacaccgaa
     7981 cagcagatgt ggcccgaggc tgcggatctt ctgcaagagc tggcggctgc cgaaaccaat
     8041 gaaaagcgac gggctcgtta caaacagacc gccggcttta tcacccgcga tcacctcaaa
     8101 gagccgcggc gggcgctgcg cctgctctgg agttcgtttg acgacgatcc atcgctggtc
     8161 aagagcctcg agtcagccga agcgctggcg gcccaaatcg gcgaccccaa agagctgctg
     8221 cgggcgatgc agcgacgaat caaagtcatg ggctccgatg cgcaggattc cccgaaacag
     8281 cgagcggaaa ggctgcggtt gtggacggaa gtggcgcgcg tctgcatcca ggaactgggc
     8341 gatctgaaga ccggcatggc cgcctacgaa gtcagtctcc agctcgatcc gcagaacatg
     8401 gatcggcacc gtcagatggc ggcgatctac gcaagcgccg gggacgacaa gctcgacaaa
     8461 gcgatcgccg agcaccacct cgtgcttaag tcaaacaaga gcgagcttgc gtcgttccgt
     8521 gcgctgcgtg atctgtatgg tcagacggga cagcgcgatc gggcagcaca ggtcgcttac
     8581 gccctgcatc ttctgcgaca ggccgagccc gaagatgaca agctggtcga cgagctaaag
     8641 tcccgcccag tgacgctggc gaggcgaccg ctgaccaaag agctgtggcg cctgcttgcc
     8701 caccccgaag aagatgcgcg gcttcagtcg ctgctcgcgg tgctgtggcc ggcgctgcaa
     8761 aaaagccatg cccgtccgtg gactgagcag ccgtttgcgc gtgacagccg ggtcgaaatt
     8821 ggctcgtcgc aggtctatct gaaggcgctg cgctatgcgt tcgagatgac tgaagcgccg
     8881 ctccccgagc ttttcccaag gccgcaagcg cgcgagctgg ccgaacaagc gttcctgatc
     8941 tcgatcgcga gtgaaggcaa gggaaccgcg acggtctgcg ccgagctggg accgccgctg
     9001 cttgacccga gccgtcccga gcgcgaaacg ctgtacgaag tcggtcgaat ggcggcgctg
     9061 ctgcgtccag aaagggcgct gcgcacagtg ctcaccgacg agctggagct tgcgcaaatc
     9121 ctcgaagcgg cgctggtcct gacccatcag gaactgagcc agaccacgcc cccagcgtcg
     9181 gcacaagtgc tggaactggc ccgctcgctg cgcggtgccc tgacggcgca gcagctcgat
     9241 cagctgagcc gcatcgggcg cagcctgctc gacagcagcg tcaagccaca acagctggcc
     9301 agcacctgga tttccgcgtc ggaacttacg acggtgcgcg tcggtcttct gctgtcgggt
     9361 gacttggaaa cggcggcgct tctgctcgcg actgatccgc cgggtctgac ccttctgtca
     9421 ccgaaacagc ggctgctgga gctgctctac tttgcggtca gtgaagaact ggcctcggtt
     9481 cgtcagttcc tcggtctgta gccgccacac ctgccctcgc ccccgccgct tgccatcccc
     9541 gctcggctaa caaagcggct atacttcata aacatggact cggatgagca gaaccgctca
     9601 gcgcggccag ggcgaccgca gctacgtttg gttccccccg agcccgccga atccaccggc
     9661 gaggtttcag gcatcgcgcc cagcaaaagc gcttccgcag cgcagattcg cgcgctcccg
     9721 gttggtcccg gcacgccgtc gctgcgtgag tcatacgtgt ttggcgccaa gatgcttttg
     9781 gatcagcccg ccgcctgccg acacggctac cgcctgtttt gggtgcatgg gcgcgacctc
     9841 ggctggatcg atctggaagc gtcttcgtcg gtgttcgcgg tgatgggctg gcacgaaaac
     9901 tgtgatcttg tgctgcccaa gctgccaaac attactaacc gccacctgct cgccagctgc
     9961 gcgcttttgg ccgacaagac accggcgctg cggctgctcg atctgcacac caaccagccg
    10021 tttaccatcg aaggacagcc gcgccagtcg atcggggtaa gtggcccgct gttgattgga
    10081 cttggcgact gcgcgatagg ctgtgtgccg ctgccggaat ttcttcagca gcgcggtcga
    10141 ctcgccgacg gggtggaact gccaagctac gacattgttg aagccgagca gccgccacag
    10201 cattccctgc ggatgcagcg ccgcacccac attacgcttt tgccccgctc gcagcgcgtc
    10261 gaggatcttc agtcgggtcc gaaagtcgcg accgcaccgg ctggccgagc ccgcgtcacg
    10321 ctgcgccggc agaccaaggc tgtttccgtc gagctgtcgg aagccgatct gtatgccggc
    10381 gtgatcatcg gtcgatccga gacgtgcatg gaccgcggtc tgcgccaagt gctgtcgggt
    10441 cagatttcgc gtctgcatgt gatgctgctg gccgaacgcg gtcgggtcta cgcgatggat
    10501 ctgtgcagca ccaacggaac ctggcagcac ggccagcgcg tccgccgcgt cgagcttccc
    10561 gatcaaggca cccagctttt gttgtcgggc aacgactcca aagtcgagct gacctggcac
    10621 ccacgacagc ccaacatccg atcgacccgc gccgaaggcc ccgctgggga ttaggcaccg
    10681 aatcaaacca tgacgacgta ttcctcgact ctcgatcgcg cgctcgcgcg gagcgccaca
    10741 gcccaccgca accaagcccg caagggttcg gacatcccgt acatcatcca tcccgtccac
    10801 gtcgcaatga tccttctgcg gcacgacttc cccgaagaag tgatcgtcgc tgcgatcctg
    10861 cacgatgtgg tcgaagacac cggaaccaca ctcgaagagc tggccgccga gttcggtccc
    10921 gaggtcgcgc ggctcgtcgg tgcggtgtca gaacaaaaaa gtgaaggtgc gcagccgctg
    10981 ccgtggcggg ttcgcaaaga agcccagcta gaacatctgc ggcatgccga tagacacaca
    11041 gcggcgctca agaccgcgga cgcgctgcac aacctgcggg caacgcttgt cgacctaaag
    11101 aaccaaggcg atgcggtgtg gacccgtttt cgcggctctc gcaccgatca tctttggtac
    11161 tacacgtcgc tgtccgagat tttgcgcccg atcctcgggg aacacccgct gtccgtcgag
    11221 ctgtcccacg caatccgcga gctacgcgag acccaaaagc tgacctaact acctgccgcg
    11281 gcggacgtgg gacgccatgc gctcgaccaa aaagtgatct tgttcgctct tgagcagcgc
    11341 cccaatgagc ggcggcaaaa tctttccagg gtctcgactg cgcagcgctt ccggcggcag
    11401 atcctcgcag actagcagct tgatccagtc gagcagctcc gacgtgcttg gccgtttttt
    11461 gagccccggt acttcccgta gctcgtaaaa caccgacagc gcttcccgca gcagcgacga
    11521 cttcagcagc ggatagtgca ccgacacgat ttttgtcatc gtttcagcgt cgggaaaacg
    11581 gatgtagtga aagaagcagc ggcgcaaaaa agcgtcgggc agttcttttt cgttgttcga
    11641 cgtgatgatg acgatcggac ggtttttggc cgcgatggtc tgcttggtct cgtagacgta
    11701 aaactccatc cgatcgagtt ccgtcagcag atcgttggga aactcgatgt cggccttgtc
    11761 gatttcgtcg atgagcagca ccggcgcgct gtccgccgca aacgcttccc acagcttgcc
    11821 gcggacaatg tagtgcgcaa tgtcatgcac ccgcggatcg ccaagctgcg agtcacgcag
    11881 ccgagccacc gcgtcgtatt cgtacagtcc gtgctgggct ttggtcgtcg acttgatgtg
    11941 ccagcggatg at
//
LOCUS       DAORGN010000156        11828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2268_length_11828_cov_6.239871, whole genome shotgun sequence.
ACCESSION   DAORGN010000156 DAORGN010000000
VERSION     DAORGN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_11828_cov_6.239871"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1250
                     /locus_tag="PKL17_15615"
     CDS             36..1250
                     /locus_tag="PKL17_15615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46213.1"
                     /translation="MTDLKPDNVIIVPDSEAPGGERAKVLDFGIAKISDQQASGLTQM
                     GEVMGTPRYMSPEQCKGSGQVDAKTDVYALGIIMFILLTGKPPFDAEGTGALMAMHIY
                     HEPPKLREVEPKVPPKVEELVLQMLGKKPDERPSMADVVARLGQLGGQSLATGAMPVL
                     RLQDIQQAQMQMQMQMGGQMASGGYPMVPHGMMGGYPGMPQGMPGAPGMHPGFQHGGP
                     QHGGPQGGQVINPAPAEGTGPNTSSRPSLLGVGQTHDPTKMGGPRSGVRRLVIPVVMA
                     TVLSAIGIVFVLRSGAGDQPPPRVTKPAAPKPIVWEIKTDPPGVQVIRTSDDAVLGQT
                     PWRSEQAPAGGKVGVILRMSGYQDKSVVLEKDQDFSQEIKLDAVPPPPTKPAKIKKKK
                     KKEESDEFGLMK"
     gene            complement(1254..2807)
                     /gene="rny"
                     /locus_tag="PKL17_15620"
     CDS             complement(1254..2807)
                     /gene="rny"
                     /locus_tag="PKL17_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNK46214.1"
                     /translation="MTIAIGIALCILGVVLGVVLAQRLSPAPRGEHAEDARQHLLDAA
                     RTEAEQIKRDAQVESKELLLRAQEAAEAEMRSRREEIEREAQRLGKQEAAQTRKAEIL
                     ASREEEQNRRERNLQNREQSTEQAAKKAEDFAEQAAKKLEQIAGLTVEQARAKLADDV
                     REDARRQAAIDVKRIEEEYRQQAEDRAKTIIATAVQRYASEYVAEKTVSVVPLPSDDM
                     KGRIIGREGRNIRALEAATGIDLIIDDTPEAVIISCFNPVRREIAKLALTRLIADGRI
                     HPTRIEESVQKASEEIEQLCKEAGEQAAFDLGLHKMHPELLRSLGKLKFRSAGAQNLL
                     QHSIEVGWLAGAMAAELNINVKLARRAGLLHDIGRAVDQDSEGSHAMVGAALCKKLGE
                     PQKVVDAIASHHGERPPTSALDYLVDAANVLSNARPGARREQLESYIQRLGNLEKLCQ
                     TIPGVDKAYAIQAGRELRILVENDKISDEQAVLLSRDITRKIEEEATYPGQVRVCVIR
                     ETRSTDYAR"
     gene            complement(2804..3442)
                     /locus_tag="PKL17_15625"
     CDS             complement(2804..3442)
                     /locus_tag="PKL17_15625"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNK46215.1"
                     /translation="MALAAFGTIADKKSRLRSELRQRRKELGFAVGSEPSRNAASSVA
                     QMALSIPELRAARCVAVYAARGHELDPSPLVRALCLSALVSYPRVEQQKPPRLSFRTV
                     SPDALVPAAFDLLEPPADAPLSPPIDVFLVPGLGFDRCGRRIGQGGGYYDAALRASPS
                     ALRVGIGYDFQLVSEIPVEECDEPLDYIVTPTQKLATLVRASGSASAKEDIS"
     gene            complement(3451..3744)
                     /locus_tag="PKL17_15630"
     CDS             complement(3451..3744)
                     /locus_tag="PKL17_15630"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNK46216.1"
                     /translation="MKRTIKVHIAGQPYQVRSDADEAYVQSLAEMVNGRVRALLGNRL
                     VATQSDMVLTALQLADELEHERRALKALRSQLRDHARRMLDYLRRLAEAPAKG"
     gene            3990..5633
                     /locus_tag="PKL17_15635"
     CDS             3990..5633
                     /locus_tag="PKL17_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46217.1"
                     /translation="MRSERALGTLGRFLRRFSFVGIAAGIAVGALWNGCAGSNTDYNG
                     NIVDGGYNGDGPPPGSDLTCAKTPDPDVPDSNFVDNNCDGIDGDYTQAIFASPTGDDT
                     NAGTRETPVKTIAQAIKLAQQQGLPSVYLDKGLYSGSVTMVAGISIYGGYDSGNLWSR
                     AAANDSQIVGGTTAVTGTNINKETHLELVSVKSADGTSPGQSSYGVFIVNSSGPVILG
                     QLKVNSGIGANGLSPTTPADVITPAAADAPAGQNGCTGGGCNGGAGSSGGVNTCNGAG
                     VSGGKGGNGNSGGGGSNGFGGSGASPGAGGSGGSSGSCFLGLYSGNPGSAGSDGAAGV
                     DGTLGAQPVAVTLANFTAAGYSPASGGDGKQGTPGSGGGGGGAGGGGSGALTCTPDTG
                     GGGGGGGAGGCAAAVGLGGGGGGGSFGLYVFKSTVTINTATIASGQAGNGGNGANGAV
                     GGRAGNGSAAGGGSVDAAGGGRGANGGKGGNSGAGAGGTGGPSYGIYAQGAMVTTNSV
                     QASAGAFGSAGNGGKATLKGVLSQAPAGFVGQAGPQKFD"
     gene            complement(5651..6118)
                     /locus_tag="PKL17_15640"
     CDS             complement(5651..6118)
                     /locus_tag="PKL17_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNK46218.1"
                     /translation="MLTLMAACHILLLALSATPDVATEAGQAAVFGYEGDELAGGPLA
                     CTGKALRPGQLVCAHRTLPCGTRLVVQNMSTQRLATCLVLDRGPYGASLPGGRFVIKR
                     NAAQRGTWRGVVDVSPSVAARLGMRRSVANVRLVYLKEKRSRESGQSRRRPAV"
     gene            complement(6146..7132)
                     /locus_tag="PKL17_15645"
     CDS             complement(6146..7132)
                     /locus_tag="PKL17_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNK46219.1"
                     /translation="MSANEMTPKPAFLQPQDFEKAIRSPDRRLLWDGGMGTALIARGL
                     RLSDESPESWLWTHPEQVAEVHRGFAEASACVLQTNTFGLVRLLVGGQLPIDPATGRP
                     ASLGDTVDRAVALALTAAAACGKPAPALVAAIGPTGQPGIAAERLAAAYREVATELVR
                     SGVSAIHLETCFDPVELRLAVRAVREVASALPLLVSLTVTHGQQGLETPLGVPLFRML
                     RELVDDPPALIGANCSQNADRMRGAVAELAQWAQSEGRRLPVLARPQLHSGAPDCKRK
                     PMEPSPQRFAEQLVRLFDEGATAVGGCCGVTAAHLAAVPQAIEGFLSPLTTL"
     gene            7337..8317
                     /locus_tag="PKL17_15650"
     CDS             7337..8317
                     /locus_tag="PKL17_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46220.1"
                     /translation="MSSLTGTWSWLYGALSVALLGAAAVPARSQRQAVWLVGSSALAA
                     AVTLVRAQTGETVAGPAIFAWSLILALYLAQSTSSRWQSFVTIVVGSLLGIAAQLLPD
                     TMEGQGLLLSQPLWIANGVAGGCVGLLVGVSTVGRHLVLKTAPVEGELRALLPPADSD
                     DELAKLVRQALLTYEEAVASLEEHEAAKAAASQLASKIGRFGKRWQDIETQAKKSNRE
                     QLTARKNELRNRIDSSSDESIRLEYTRALAAIDEQLSYLDDIAKGRERAIARLHHQVA
                     TLERLRLAAVRHHSVGAAKLGEELKSVVEELNQAGQDLDTAVEVLAELPN"
     gene            complement(8322..8867)
                     /locus_tag="PKL17_15655"
     CDS             complement(8322..8867)
                     /locus_tag="PKL17_15655"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNK46221.1"
                     /translation="MFVRLHSKPSESVDQQADPIAMLLACHQRIRDHLRLAQRLVDAV
                     EPKAQEVADAASALLRYFGTALPLHILDEDETLCSALSEPWLPETCAVALAQMRDEHL
                     VIEPVIEEALVGWRRLVSEPLELAALRDGLQLLTLRLGALLDAHLDAEERLIFPVASQ
                     VLTADTKARLSEAMRARRQSG"
     gene            complement(8923..10005)
                     /locus_tag="PKL17_15660"
     CDS             complement(8923..10005)
                     /locus_tag="PKL17_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK46222.1"
                     /translation="MRQVREHSPVGQGCSQRGGMRRASFLAGLVAAGITLLTAATGFA
                     QVQNSAGPAGGRAGALPPVNYPIDEFGTGQVGTTPMTPASQTGGKSEKACCTPGKDCP
                     CTAGKDCACTPGVNCPCTPGKDCACTPGVNCPARPPLRSDFCTPGKNCPCSGDSCRYC
                     TPGKDCPCTPFINCPDDHGVIFEKNAGQVSDENRQKLSRAVEELKKLPEGEMIRIEGH
                     TTPSEAGGSPPARLKLSQDRARAVEQALLQAGLPPSRLAPGGTRGWGGLCPQVLNPED
                     EKNRRVQFMFSHDVTLCAHAESQRRGANLPPYKKPPQKTGTGVKPAGAQQVKPNPTAT
                     APKPATGAATAPKPAAATAPKPPGTK"
     gene            complement(10053..>11828)
                     /locus_tag="PKL17_15665"
     CDS             complement(10053..>11828)
                     /locus_tag="PKL17_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK46223.1"
                     /translation="PRARSRACNGLTSMSNAEHRPVSDTLFARLRFRLATLLCAAGLC
                     VTQAASAQDASRNALDINRFQPAFGTGRLVTQDLAELLPRFEIAPQLFLHYSKNPLYL
                     YMGDQPRFPLVGDRLTGDLGIAVGIPIRGTGRFQFGLSLPVTFWQTGEVGRIGEEFKD
                     PALRASLPSQEPSSIGQEDLRFQAKVVFLNGKWGGLGFAGDLKLPTGDKQSFLGSPLP
                     TGNLKLLFHLNLWRFTLVLNPGVYLAQAQQIGFTSVGNSLSVGAGLNLRLYNWNHGSL
                     DVMTEVFGLANFNFRSLGETPLESTVAGRVNIETQTAGDFHIYLGGGPGVPATGGAKG
                     IGSPDFRVYAGLVWSWNKKPTPPPPPPPPPAPDCRCRGANCPCIPGVNCQCTPGVNCP
                     CTPGVDCPCTPGKDCVCEEGKTCACTPGVNCPCTPGVNCQCKPGVDCPKKNIKIAGSL
                     FEFDSPVLTPDGLETIRRSVDAIADHINKGNRIRIEGHTDNVGGLDYNTRLSRARAES
                     VADFIRSELGVKGIPADVTQRSVLVGWYAFNCKFVVDSPLSAQRKLTPDKTKQRDREN
                     EPNRRVEINLWPDDSIKCFVPLPPQ"
BASE COUNT         2203 a         3728 c         3709 g         2188 t
ORIGIN      
        1 attgacatcc caccgacggt agacaagaaa atgtgttgac agacctgaag ccggacaatg
       61 tgatcattgt gccggattcg gaagcgccgg gtggggaacg ggcgaaagtg ctcgacttcg
      121 gcatcgccaa gatcagcgat cagcaggcga gtggcctcac gcagatgggc gaggtcatgg
      181 gcacgccgcg ctatatgtcg ccggagcagt gcaaaggatc ggggcaggtc gacgccaaga
      241 cggacgtcta cgcgctcggc atcatcatgt ttattctgct cacggggaag cctccgttcg
      301 acgcagaagg caccggcgcg ctgatggcga tgcacattta tcacgagccg ccgaagctgc
      361 gcgaggtcga gcccaaggtt ccgccgaagg tcgaagagct ggtgctgcag atgctgggca
      421 aaaagcctga cgagcgcccg tcgatggccg atgtggtggc gcggctcggg cagctcggtg
      481 gacagagcct ggcaacgggt gcgatgccag tgctgcgact gcaagacatc cagcaagccc
      541 aaatgcagat gcaaatgcag atgggtgggc agatggcctc gggcggctat ccgatggtgc
      601 ctcacggaat gatgggcggc tatccgggca tgccgcaggg aatgccgggt gcgccgggaa
      661 tgcatccagg gtttcagcac ggcgggcctc agcacggcgg gcctcagggc ggccaggtga
      721 taaatccggc gcctgccgag ggaactgggc ccaatacttc atcgcgaccg tcactgcttg
      781 gggttggaca gacccacgat ccgaccaaga tgggcgggcc gagatcgggc gtccgtcgcc
      841 tggtcattcc ggtggtgatg gcgacggtgc tgtcggcgat cggcatcgtg tttgtgctgc
      901 gcagcggcgc cggcgatcag ccgcctccgc gcgtcaccaa acccgccgcg ccgaaaccga
      961 ttgtgtggga aatcaagacc gacccgcccg gtgtgcaggt cattcggacg tccgacgatg
     1021 cggtacttgg acagacgccg tggcgttccg agcaggctcc ggcgggcggc aaagtcggcg
     1081 tgatcttgcg gatgtccggc tatcaggaca agtcggtggt gctcgaaaaa gaccaggact
     1141 ttagccaaga gatcaagctc gacgcagtgc caccgccacc gacaaagccg gccaagatca
     1201 agaagaagaa aaagaaggaa gagtccgacg agttcggact gatgaagtag cgtctagcgt
     1261 gcgtagtccg tcgagcgcgt ctcgcgaatc acgcagacgc gaacttggcc aggataggtc
     1321 gcctcttctt cgattttgcg ggtaatgtct cgcgacaaaa gcaccgcttg ttcgtcggaa
     1381 atcttgtcgt tttcgaccaa gatgcgaagt tcccgccccg cctgaatcgc ataggctttg
     1441 tcgacgcccg gaatggtctg acacagcttt tccaggttgc ccaggcgctg gatgtacgac
     1501 tcaagctgct cacgccgggc ccccggtcgg gcatttgaca gcacattggc ggcatcgacc
     1561 aaatagtcga gcgcgctcgt cggtggtctt tcgccgtgat gagaagcaat cgcatcgacg
     1621 actttctgcg gctccccgag ctttttgcac agcgccgcgc cgaccatcgc atgcgagcct
     1681 tcgctgtctt gatcgaccgc ccgcccgatg tcgtgcagaa gccccgcccg tcgagccagc
     1741 ttgacgttga tgttaagctc cgccgccatt gcaccggcca gccagcccac ttcgatgctg
     1801 tgctgaagca ggttctgcgc ccccgccgaa cggaacttca acttgccgag cgatcgcaga
     1861 agctcgggat gcattttgtg cagtcccaga tcgaatgccg cctgctcgcc ggcctctttg
     1921 caaagctgct cgatttcctc ggacgctttc tgcaccgact cctcgatccg cgtcggatgg
     1981 atgcgcccgt cggcaatcaa gcgagtcagc gccagcttgg caatctcgcg tcggaccgga
     2041 ttgaagcagg aaatgatcac cgcttccggc gtgtcgtcga tgatcagatc aattcccgtc
     2101 gctgcttcga gcgctcgaat gttgcgacct tcacgaccga tgatccgccc cttcatgtcg
     2161 tccgatggca gcggcacgac acttaccgtc ttttcagcga cgtactcact cgcgtagcgc
     2221 tgcaccgccg tcgcaatgat cgtcttggcg cgatcttcgg cttgctgacg gtactcttct
     2281 tcgattcgct tcacgtcgat cgccgcttgc cgcctggcat cttcgcgcac atcgtcggcc
     2341 agcttggccc gcgcctgctc gacggtcaac ccggcgatct gctccagctt tttggcggct
     2401 tgttcggcga agtcttcggc ttttttggcg gcttgctccg tcgactgctc gcggttttgc
     2461 aagttgcgct cgcgacggtt ctgctcttct tcacgcgatg ccaatatctc ggctttccgc
     2521 gtctgcgccg cttcttgttt gccgagccgc tgcgcctccc gttcgatttc ttctcgacgg
     2581 gaacgcatct ccgcttcggc ggcttcttgg gcccgcaaaa gcagctcttt cgattcgacc
     2641 tgcgcgtctc gcttgatctg ttccgcttcc gtgcgggcag cgtcaagcag gtgctgtcgc
     2701 gcgtcctcgg cgtgctctcc tctcggtgcc ggagacagcc tttgcgcgag cacaacgccc
     2761 aggacaaccc ccaaaataca cagggcgatg cctatggcga tcgtcacgat atgtcctcct
     2821 tagccgacgc tgatccactc gcccggacca gggtcgccag tttttgcgtc ggggtcacga
     2881 tgtagtccag cggttcatcg cattcctcga cggggatttc tgaaacaagc tgaaaatcgt
     2941 agccgatacc aacccgcagc gccgacggag acgctcgcag cgcagcatcg taatagcccc
     3001 cgccttgccc gatccgtcga ccacagcgat cgaaaccaag tccgggcacc aaaaacacat
     3061 cgatcggggg actcagcggc gcgtcggcag gtggttcgag cagatcgaac gccgccggta
     3121 caagcgcgtc gggtgaaacg gtacgaaacg acagacgcgg cggtttttgc tgttccaccc
     3181 gagggtatga aaccagcgcc gataggcaga gcgcacggac gagcggggac gggtctagtt
     3241 catgaccgcg cgccgcatac acagcaacgc agcgagcggc ccgcaattcc ggaatcgaaa
     3301 gcgccatctg cgcgacggaa gaagccgcat tccgcgacgg ttcggagccc accgcaaagc
     3361 ccagctcttt gcgccgctgc cgaagctcgc tacggagccg cgatttcttg tctgcgatgg
     3421 tgccaaaagc agcaagagcc acaaccacac ctagcccttc gcgggtgcct cggcaagccg
     3481 tcggagatag tcgagcatcc gtcgcgcatg gtcgcgtagc tgcgaacgca gcgcttttag
     3541 cgcacgccgc tcgtgctcaa gctcgtctgc cagttgaagg gcggtcagga ccatgtccga
     3601 ttgggttgct accagtcgat ttccgaggag cgcacgaacg cgcccattca ccatttctgc
     3661 cagggactga acgtacgctt cgtcggcatc gctgcggacc tgatacggct gaccagcgat
     3721 gtggactttt atcgtacgct tcacagcagg accgacaata tcgagaggta cgacaatgtc
     3781 aagcaggctg cgtccgctcc gatcatggtg caggtcttgg tctggctttg cggaccgcgc
     3841 gggggacttg gctgcgcttt ccccaaatcg gcacccccag ggccaaataa cggaaaccga
     3901 tgggtttatt tgtaatcttt attactaatt tttcgtttga tacactcctc agaccgcctc
     3961 tcgtcactta accagcctta ggagggcaca tgcggtcaga gcgagcgttg ggaaccttgg
     4021 gtcgcttttt gcggcgcttt agcttcgtcg gcatcgcggc aggcatcgcc gtcggcgcgc
     4081 tatggaacgg ctgtgccgga tcgaacaccg attacaacgg caacatcgtc gatggcggct
     4141 acaacggtga cggtccgcct ccgggcagcg atttgacctg cgcgaaaacg cccgatccgg
     4201 acgtgccgga ctcgaacttt gtcgacaaca actgcgatgg catcgacggc gactacaccc
     4261 aggcgatttt cgcgtcgcca accggagatg acaccaacgc cggcacccgc gaaaccccgg
     4321 tgaagaccat cgcgcaagcc atcaagctgg cgcagcagca gggtcttccc agcgtctacc
     4381 tcgacaaggg actctacagc ggttcggtga cgatggtcgc cggcatcagc atttacggcg
     4441 gttacgattc aggcaacctg tggtcgcgcg ccgctgccaa tgacagccag atcgtcggcg
     4501 gcacgacggc agtcaccggc accaacatca acaaagaaac ccacctggag ctggtctcgg
     4561 tgaagtcagc ggacggcacc agccccggtc agtcgagcta cggcgtcttc atcgtcaaca
     4621 gctcgggccc agtcatcctc ggacagctga aggtcaactc cggcatcggc gcaaatggcc
     4681 tttcgccgac gacccctgcc gatgtcatca ccccagctgc tgccgatgca ccggcaggcc
     4741 aaaacggctg cacgggcggc ggatgcaacg gcggtgccgg gagcagtggc ggcgtaaaca
     4801 cctgcaacgg tgctggtgtc tcgggtggca agggcggcaa cggcaactcg ggtggcggtg
     4861 gcagtaacgg tttcggtggt tccggtgcgt cccccggagc aggcggtagt ggcggatcgt
     4921 ctggcagctg ttttctgggc ctttactctg gcaaccccgg aagtgcaggc tccgatggcg
     4981 ccgcgggcgt agacggcacc ctgggcgccc aaccggtggc cgtgacccta gcgaacttca
     5041 cggctgccgg atattccccg gcgagcggtg gcgatggcaa gcaaggtact ccgggcagcg
     5101 gtggtggtgg cggtggcgct ggtggcggtg gttcgggggc gctcacttgc acccctgaca
     5161 ccggtggcgg cggcggcggc ggtggcgccg gcggctgcgc ggcggcggtt gggttgggtg
     5221 gcggcggtgg tggcggttcg ttcgggcttt acgtcttcaa gagcaccgtc acgatcaaca
     5281 cggcaaccat cgccagtggg caggcaggca acggtggcaa tggcgcaaac ggtgcagtag
     5341 gcggtcgagc aggcaacggc tccgcggcag gcggtggctc ggtagacgcc gcaggcggcg
     5401 gtcgtggtgc caacggcggc aaaggcggca actcgggtgc gggcgctggc ggcacgggtg
     5461 gcccaagcta cggcatctat gcgcagggcg cgatggttac gaccaacagc gttcaggcct
     5521 cggcgggtgc attcggcagc gccggcaatg gcggcaaagc aaccctcaag ggagtgctgt
     5581 cgcaagcgcc agcgggtttc gtcggacaag ctggcccgca aaagttcgac taatccctct
     5641 ccgttcgatg ctagacagcg ggccgcctgc gggactgccc gctctccctc gaccgcttct
     5701 ccttcaaata aaccagtctc acgttggcaa cgcttcgccg cattcccagg cgagcggcca
     5761 ccgatgggga cacgtcaacg acgccgcgcc aagtgccgcg ctgcgccgcg ttccgcttga
     5821 tcacaaagcg tccgccgggc agacttgcgc catacggtcc acggtcgagc acgaggcaag
     5881 tcgccagtcg ctgcgtggac atattttgca cgaccagccg cgttccgcac ggcagcgtcc
     5941 gatgcgcaca gaccagttgt ccgggccgca gcgcttttcc agtacatgcc agtggccccc
     6001 cagccagctc gtctccttcg taaccaaaca ctgccgcttg ccccgcttcg gtcgccacat
     6061 ctggggtcgc ggacagagcc aggagcagga tatggcaagc cgccatgagg gtcagcatcc
     6121 aggcctcctt gtgtagattg cgccttcata gcgtggtcag aggggaaagg aaaccctcaa
     6181 tcgcctgcgg aacagcagca agatgcgccg cggtcacgcc acagcagcca ccaaccgccg
     6241 tcgcgccttc gtcgaacaat ctcacgagct gctcggcaaa tcgctgcggg gatggctcca
     6301 tcggcttccg cttgcagtcg ggcgccccgc tgtgaagctg aggtcgcgcc agcaccggca
     6361 gtcgtcgacc ttccgactgc gcccactgtg ccagttctgc gactgcacct cgcatccgat
     6421 cggcgttctg agagcagttt gcgccaatca gcgcaggcgg atcatcgacc agctcacgca
     6481 gcatccgaaa aagtggcacc ccaagcggtg tttccagtcc ctgctgtccg tgcgtcaccg
     6541 tcagcgaaac cagcagcggc agcgccgatg ccacttcgcg caccgctcgg acggccagtc
     6601 gcagctcgac aggatcgaaa caggtttcca gatggatcgc cgacacaccg ctgcgcacaa
     6661 gctctgtggc cacttcacga taggccgcag cgagccgctc tgctgcgatc cctggctgac
     6721 ccgtcgggcc gatcgctgcc accagtgccg gtgccggctt tccacaagct gccgctgccg
     6781 tgagggccag cgcgaccgct cgatcgaccg tgtcacccag cgatgcgggc cgccccgtcg
     6841 ccggatcgat cggcaactgg ccgccgacca ggagccgtac aagcccaaac gtgttcgttt
     6901 gcagcacgca ggccgacgct tcggcaaaac cccgatgcac ctcggcgact tgttcaggat
     6961 gcgtccacag ccacgactcc ggtgactcgt cggaaagccg cagaccacga gcgatcagcg
     7021 ccgtgcccat cccaccatcc cagagcagac gccgatcagg cgatcggatc gctttttcaa
     7081 agtcctgcgg ctgcaaaaat gccggtttgg gagtcatctc gtttgcgctc acaattttct
     7141 ttgacagcga cgggaaatgg ttctgctcgc ctagaccgaa tcagcggaat atgcgttgat
     7201 tgaagtcatg aagcgaagtt tggcgatcga caatcacgta gcgttccagt ggtccgtggt
     7261 tcgttccgtt gtggcgggtg gcctgcttgg gctcgttcac accgggatgg ccgctagcaa
     7321 tagcacgctc agcctcatgt cctcgctgac aggtacatgg tcgtggctgt acggcgcact
     7381 cagcgtcgcg ctgctcggtg cggcagcggt gccagcgagg agccagcgtc aagcggtctg
     7441 gctcgttgga agctccgcgc tggcagcggc ggtaaccctt gtgcgagccc aaacgggcga
     7501 gacggtggcc ggcccggcga tcttcgcgtg gtcgctgatt ctggcgcttt atcttgcgca
     7561 gagtacgtcg tcccggtggc agagctttgt gacgattgtc gtcggctcgc tgcttggaat
     7621 cgccgcgcag ctcctgcccg acacgatgga aggtcagggt ctgctgctca gccagcccct
     7681 gtggattgca aacggtgtcg caggtggctg cgtcgggctt ttggtcggtg tctcgacggt
     7741 cggtcggcat ctggttctga aaacagcgcc ggtcgaaggc gaactccgcg ctcttttgcc
     7801 accggcagac agcgacgatg agctggccaa gctggtgcgt caagcgcttc tgacctacga
     7861 agaggcggta gccagcctcg aggagcatga agcggccaaa gcggcggcgt cgcaactcgc
     7921 gagcaaaatc gggcgtttcg gcaagcgctg gcaggacatt gaaacgcagg cgaaaaagag
     7981 caatcgcgaa cagcttactg cccgcaaaaa tgaactgcgc aaccggatcg attcatcttc
     8041 cgacgagagc attcgtctgg aatacacccg cgctctcgct gcgatcgacg agcagctaag
     8101 ttacctcgat gacattgcga aaggtcgcga gcgcgcgatt gcccgactgc accaccaagt
     8161 ggcgacgctt gagcgtctgc gactcgccgc ggttcgtcat cactccgtcg gcgcagccaa
     8221 acttggcgaa gagctaaagt ctgtcgtcga agaactgaat caagccggtc aagacctcga
     8281 caccgccgtc gaagtgctcg ccgagctgcc aaactgacaa gctagcccga ctgccgccgg
     8341 gcccgcatcg cttccgaaag tcgcgccttc gtatccgcag tcagtacttg cgacgccact
     8401 ggaaaaatca gccgctcctc cgcatccaga tgagcatcca gcagcgcgcc aaggcgcagc
     8461 gtcagaagct gtagtccatc gcgcagcgca gccagttcta gcggttcgct cacaagccgt
     8521 cgccatccaa caagcgcttc ctcgatcacc ggctcgatta ccaaatgttc atcgcgcatc
     8581 tgggccagcg cgacagcaca ggtctcgggc agccatggct ccgacagcgc gctgcaaagc
     8641 gtctcatcct catcgagtat gtgcagcggc aaggccgtcc cgaaatagcg caaaagcgcg
     8701 gatgcggcgt cagcgacttc ttgcgctttt ggctcgacag cgtcgaccag ccgctgggca
     8761 agccgcaggt gatcacggat tcgctgatga cacgcaagca gcatcgcgat cggatcggct
     8821 tgctgatcga cgctctcgct gggtttggaa tgtagccgga cgaacatgct ggggcttgat
     8881 ggcgtccttg tggcgaacgc caggcaaaag ggatcgctgc gcttacttcg ttcccggcgg
     8941 cttaggcgcc gtcgctgcgg ctggcttggg cgccgtcgca gcaccggtgg ctggcttggg
     9001 cgccgtcgct gtcggattgg gcttcacctg ctgtgcccca gcaggcttca caccagtgcc
     9061 ggtcttctgc ggcggcttct tgtacggagg caggtttgca ccccgacgct ggctttcagc
     9121 gtgagcacac agcgtcacgt catgcgaaaa catgaactgc acgcgacggt tcttttcgtc
     9181 ttccggattc agaacctgcg ggcacagacc gccccagcca cgggttccgc ccggtgcgag
     9241 ccgggacggc ggcagacccg cttgcagaag cgcttgctcg acggctcgcg cccgatcctg
     9301 agataacttc agacgcgctg gcggcgaacc accggcttcc gacggggtcg tgtggccctc
     9361 gatgcgaatc atctcgcctt cgggaagctt cttcagttct tcgaccgctc gcgagagctt
     9421 ctgccgattt tcgtcgctaa cctgtccagc gttcttttcg aagatcacgc cgtggtcatc
     9481 cgggcagttg ataaacgggg tacacgggca gtctttgcca ggcgtgcagt agcggcagct
     9541 atcccccgag caggggcagt tcttaccagg cgtacagaag tccgagcgaa gcggaggccg
     9601 agccggacag ttgacccctg gcgtacacgc gcagtcttta cccggtgtgc atgggcagtt
     9661 gacccctggc gtacacgcgc agtctttgcc ggcggtgcac ggacagtctt ttcccggcgt
     9721 gcagcaggct ttttcgctct taccgccggt ttggcttgcg ggcgtcatcg gggtggttcc
     9781 gacctgaccc gtcccaaact cgtcgatggg gtagttcacc ggaggcagcg cccccgcacg
     9841 accgccggct ggcccggcgg aattttggac ttgggcaaag ccggtcgctg ccgtcagaag
     9901 cgtgatcccg gctgcaacga gcccagcgag gaagctggcc cgccgcatac caccgcgctg
     9961 actgcaccct tggccgaccg gcgagtgttc acggacttgc cgcatggcta tttgtctccg
    10021 attccctttc atcgcgtcct ctctcactgc gctcactgcg ggggcagcgg gacgaagcac
    10081 ttgatgctgt cgtcgggcca caggttgatt tcgacgcgac ggttcggctc attttcccga
    10141 tcgcgctgct tggtcttgtc cggggtcagc ttgcgctgag ccgacagcgg cgagtccacc
    10201 acgaacttgc agttgaaggc gtaccagcca accagcaccg accgctgcgt cacatctgcc
    10261 ggaattccct tgacgccaag ctccgagcgg atgaagtcag cgacgctttc ggcgcgagcc
    10321 cgcgacagtc gcgtgttgta gtcgagaccg ccgacgttgt ctgtgtggcc ctcgatgcgg
    10381 atgcggttgc ccttgttgat gtgatcggcg atcgcatcga ccgagcgacg aatcgtctcc
    10441 agaccatccg gcgtcagcac cgggctgtcg aactcgaaca gcgagccggc aatcttgatg
    10501 ttcttcttcg ggcagtcaac acccggcttg cactggcagt tgacgccagg ggtgcacggg
    10561 cagttgacgc caggggtgca agcgcaggtc ttgccttcct cgcagacgca gtctttgccg
    10621 ggggtgcacg gacaatcgac acccggcgta cacgggcagt tgacgccagg cgtgcactgg
    10681 cagttgacgc caggaataca cgggcagttt gcgccacggc agcggcagtc aggcgcaggc
    10741 ggtggtggcg gcggcggcgg tggcgtcggc tttttgttcc acgaccagac caaaccggcg
    10801 tacacgcgga agtctggcga gccgatgcct ttggctccac ccgtcgctgg aacacccggt
    10861 ccaccaccca agtagatgtg gaagtcgcca gcggtctgcg tttcgatgtt cacacggcca
    10921 gccaccgtcg attcaagcgg cgtctcgccc agtgagcgga agttgaagtt cgccaagccg
    10981 aaaacttcgg tcatgacgtc gagagagccg tggttccagt tgtagagccg caggttcaag
    11041 cccgcgccca ccgacagcga gttgccgacg ctggtgaagc cgatctgctg cgcctgggcc
    11101 agatacacgc cagggtttag caccagggtg aagcgccaca ggttcaagtg gaacagcagc
    11161 ttgaggtttc cggtcggcaa cggcgagcca aggaagctct gtttgtcgcc ggtcggcagt
    11221 ttcagatcac cggcaaagcc gagaccgccc cacttgccat tgaggaacac gaccttggcc
    11281 tggaagcgca ggtcttcctg accaatcgag ctcggctctt ggctcggcag actcgcccgc
    11341 agcgccggat ctttgaactc ctcgccaatt cgaccaactt cgcccgtctg ccaaaacgtg
    11401 accggcaggc tgagtccgaa ctggaagcga ccggtgccgc gaatcggaat gccgaccgcg
    11461 atgccgagat cacccgtcag gcggtctccg acgagaggaa accgcggctg atcccccatg
    11521 tacaaataca ggggattttt cgagtagtgc aggaagagct gcggcgcaat ctcgaagcgg
    11581 ggcagaagct ccgccagatc ctgcgtgacc aagcggcctg taccgaaagc cggttggaag
    11641 cggttgatgt ccagggcatt gcgcgaagcg tcctgggcac tggcagcctg agtcacgcac
    11701 aggccggctg cacagagcag tgtggccaag cgaaaacgca ggcgcgcaaa gagggtgtcg
    11761 gaaacggggc ggtgttcggc gttcgacatg ctggttagac cattgcaagc tcgactgcgg
    11821 gcccgtgg
//
LOCUS       DAORGN010000157        11728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2285_length_11728_cov_6.716097, whole genome shotgun sequence.
ACCESSION   DAORGN010000157 DAORGN010000000
VERSION     DAORGN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11728
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_11728_cov_6.716097"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="PKL17_15670"
     CDS             <1..1990
                     /locus_tag="PKL17_15670"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HNK46224.1"
                     /translation="GYSTSLYTDLGHPVNSVRVTYSFLDLPETPLLRREADDRIGTFA
                     QPFRDFDSASPRLTAHAVMRWRLEPDHKEGPLLVPKRPIVFYLDPATPKSYQPYILDG
                     VRQWNRAFEAAGWKDAVRASLLPTGVSIDDPRAAVIHWDVGEPSFNGRGQPLVDPRSG
                     EILGASLILSMGPVRTHLRLRKTVLAPQTENQQSRSDHATINGSLLADESDLADSLSE
                     QAEVLRAALLSTDAVQSDGPLPEALIGQRLRLLAMHEAGHALGLRHNFRASSIAPSDQ
                     LGNPDWQRQSPLVSSVMDYPAINLPPPGSPSAKRGYDPSFLYYPTSLGPSDLLSIAYA
                     YTPDPGYAAQLAKQAAAWGYVFGSDQEASLGADPLVQRWDLGSDPLAWAKDRSELLRK
                     LWVDTMKTRLKKGERPAEITEQLGWLLADYARAVDAAPTYIGGRHSSRDHIGDPGGRP
                     AYRPVEKAKQTEALAFLLDAVFSEQALPISAELAQQMGDDYLDVRRRDARNQTVYPLW
                     RQVLDLRVSTLHDLLSPLRLRRMVDGEQVFGRDAVLTVGELLGKLSQSLFAEILSGTP
                     SGLSPMRRELQLQYIESLGNLVLQHAPESRHARSQARYQLQELRGKLDALRSKRDSFD
                     VDTRAHLAELEDSISATVNGAMVIRPPADSQTHSHSAE"
     gene            2259..4595
                     /locus_tag="PKL17_15675"
     CDS             2259..4595
                     /locus_tag="PKL17_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK46225.1"
                     /translation="MKRTATLRFLSHSVLAFSSFALAAGCSPAQLDEVSTEEELLATA
                     ATVKGTTLRTLGFDAASLDAENKQPVYLAGRVPAAISGAESARDFAMNLLGRSLRLSP
                     DSDFVFHKETRGEDGHRYLRLRQTHGGVPVEQGELTLQVAEDGAVRSVLGRPLPNLRI
                     STRPTLLGDAALKGALAKLVKSGNAQVHKSPELVIHTNLAGQPVLTYHAVVEYQGASG
                     LAFEDLYVDANSGQVVSQISRIHTGLSRTIYDGKKACLSTGSELPGTLLYNEGGTSTD
                     TVANAAYTNTGLTYSFYKNFHGRDSYDDKGAPLKSSVHFTFNSGFSCDGDNAAWLGDP
                     YNQMVYGDGDATMFKPLSLAIDVTAHELTHAVTDKTSSLVYSNESGALNEAMSDIFGA
                     IAEAWKASGGSASGNPASITPTANTWKIGEEIAQAGLSGGALRFMNNPTADAYSKDYY
                     PERITGSSDNGGVHGNSGIANLAFYLLSQGGSHPRAKTTTVVTGIGIEKAAKIFYQAN
                     TSILTSSANFQAARNATAQAAQTLYGSCSAEWTNTHKAWDAVGVTGTWTPCGTGDTTP
                     PTATLTAPSAGSTLTGTVTVSASATDNIGVASVDFYAGTTLIGTDTTAPYSISFNSTT
                     VANGSYTLTAKAKDGAGNVGTSAGVAVTVSNSGGGTTQNEAEPNGSYNQANTVATSGT
                     TVTGYITSSTDDDYFKVTLGAGKTLQAVLTPPSGSDFDVYIYDQSKVLVAKSENDVGL
                     VDSASATNKAASAGTFWIYVKYYSGASKTNPYKLKLTF"
     gene            4714..5196
                     /locus_tag="PKL17_15680"
     CDS             4714..5196
                     /locus_tag="PKL17_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNK46226.1"
                     /translation="MAQSAAKTVNEYLAELPADRREVIATLRELIVANLPAGYSEAMQ
                     YGMIGYAVPLSRHPNTHNGQPLAVAALAAQKGYMSLYLMSVYGDPALCAWFVAEHERA
                     GKKLDMGKSCIRFRKLEELHLPAIAEVIRRVSVEDFIRTYEAARASAASAKRKAKTAS
                     "
     gene            5286..6212
                     /gene="bamD"
                     /locus_tag="PKL17_15685"
     CDS             5286..6212
                     /gene="bamD"
                     /locus_tag="PKL17_15685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNK46227.1"
                     /translation="MHRLFRELTFTPLALRVTLLAAVLLSGGCAAFVENFRPVIYGKN
                     AKENYERGIRSLKGESSLDAAKYFNYVRQNWPTSRWVPWAELGLADAAFGREAYIEAI
                     DGYKTFIASHPRHPKTVDGYCAFKIGESYFKQIPSDFFILPPSYEKDQGPVIDAQREL
                     TSFLDRYGQSAYAESGRKLLADVIQRLADHELYVARYYMERGKTKAATWRLEYVVREY
                     PKARRDAEVLMMLGQVYLKQNEPKDARDTFRRLLIEHPDDYRVDQAKLFLDYIEKNFG
                     DLPPPAPLPPRKSPGQKGGLLPPDDSDLLPPE"
     gene            6318..8675
                     /locus_tag="PKL17_15690"
     CDS             6318..8675
                     /locus_tag="PKL17_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46228.1"
                     /translation="MANPGTKEKLIEAAKELAEKGQFDKALREYLKVAAEDENDVLVF
                     IRIGDLYVKLGQKQEAIANYQRVAQLYVGTGQLDKAVSVYQQILQLDGKSADTYMTLG
                     GLFRDQGKLPLAMQQFELAAQLYMRASRTAQSLAAEQAVVDLAPDSLPRRIRIAELYS
                     KENLPTEAAREFEKAAQLLRKQGRLDDWAKVMERLLFHAPGQIAESNELARYYLAQGD
                     GLRALSRLQASYKVNRQDRDQLELLSQTFLLLQKPDKAVAVLKEQARQTQDRKVAESV
                     YRKILEIDPTDADAQVALGGGLLPSATSSQTNFTSALILNSPSPGPVPASKSSPLPAS
                     TVSARPTPSVVPAMPARTSSDITPVVAVRVDGTLPPISRPAVEPLSDEEEQDRVLSET
                     DSFVRFGLKGKALESLRATLSHRPHLAKVREKLSKVYELLGQFKPAIAELRTLADRAV
                     SHEEQARYLREILRLDNRDPAASEKLRRISGEFKPIAPPADEDEPVIEMSAEDDGPTT
                     PNSLPKSEPQALPPAPSGPSSRGAQTENSGSKALPSQRSGTLREELAEVDFFLQQRMF
                     DDAKRLLRSLATRYPHSQTVQNKLSELSVSASSSDENLIELEPEELVSDSNPNVGKKL
                     TGVARPPEKKAPPSASARSIPVPPSVTSSGPAEKSGPISETRDPAEAYQRGIAHRNRG
                     QHALAIGEFKAAMQDRKLAGRAALMLGQCYRDLSQLREAVEAFKEGLYMPSVADADLA
                     EMNYQLAQCYERLQNPKEAIYFYQQAQQAQAGYKDTAARLSTLRK"
     gene            complement(8676..9659)
                     /locus_tag="PKL17_15695"
     CDS             complement(8676..9659)
                     /locus_tag="PKL17_15695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNK46229.1"
                     /translation="MSESKPPVEPSHGGGLRVVLVALCINLLIAVFKFVAAGISGSTA
                     MLAEAVHSLADTANQVFLLIGMKRSSRPPDARHPFGYGTETYFWAFIVAGSIFLIGAA
                     VSIWEGSEKLWHILHGQPIAHGNIKWAVIVLSVSLVLEGWSLRAALQEFKHMTAGKGL
                     RKTVEDARDPTVLTVLFEDMAALFGLFAALVGVVLSYVTGNLMFDALASIVVGVALLV
                     VALFLGRDSMSLLIGEAVPKEEHEQIVALAAAHPGILQVIHLRTKHIAPQEVLATFKI
                     RFARDLTMDGLEAKINDLEAELRAKFPHLRRIYIEPGFDEATLRKEQGIPY"
     gene            complement(9672..10331)
                     /gene="yihA"
                     /locus_tag="PKL17_15700"
     CDS             complement(9672..10331)
                     /gene="yihA"
                     /locus_tag="PKL17_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNK46230.1"
                     /translation="MQILRSDFVASAPSRSSWPAESLAEVAICGRSNVGKSTLLNALL
                     GRRGLARVSRTPGRTRLINFFSAKLKGPRDPLSVLLADLPGYGYAEVSRSERQSWQKM
                     MEEYFEKRSNLRAVMLLCDARRVLDPRVEELLFDETELSTYLRGLDRQVIPVLTKADK
                     LAKSERKPAAQLLSRLLGGRAVVCAAEQGEGIEELWLRVVSSVASPSGAGHLVDSETA
                     L"
     gene            complement(10363..11685)
                     /locus_tag="PKL17_15705"
     CDS             complement(10363..11685)
                     /locus_tag="PKL17_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNK46231.1"
                     /translation="MSSVPLLPKVAITGLGLVSPFGAGQTSFFSALADGRSALALHPE
                     LSSTLPGDLARQPVWAGRVPDFGADKAIEAGKRRRMPRLSQMAIVAAREAMGLQPGAA
                     SQDAALYRRYRPDRLGVVLGTGLGCVEYTIDFVRSFLSGGLAAASPAVFPYTVMNASA
                     GLVAIELALTGPNITVNHRELSLAESIATGAELLLTNRADAVLCGGCDELGPWLYHGL
                     WRLGALAPFLNEGDGAPLHPYESGSGGIVPGEGAAIVLLERADAETSAPCLGYLSGWA
                     RAGDSRHRIGWPTVAASDGKDPSHPPSAHLFSGAAQSIVQSVQSAGLRPEDVSYVAGA
                     GNGTACDAIETAALRAALGQVAERVPISSILGQCGESLMSTGLRFGAALSALRSATLP
                     GTTHCRTPDKTCSLPGLVRFPTPSPVEHVLIPTIAQGGGNVSLVVSRA"
BASE COUNT         2402 a         3746 c         3497 g         2083 t
ORIGIN      
        1 tggctattcg acgtcgcttt ataccgacct gggacatccg gtaaattccg tgcgcgtcac
       61 ctattcgttt ctggatctgc cggaaacgcc gcttttgcgc cgcgaggccg acgatcgcat
      121 cggcaccttt gcgcagccgt tccgcgactt cgattcagcc agtcctcgcc tgactgccca
      181 tgcggtcatg cgctggcgac tggagcccga tcacaaagaa gggccgctgc ttgttcccaa
      241 gcgaccgatt gtcttttatc tcgatcccgc cacgcccaaa agctatcagc cctacattct
      301 cgacggggtg aggcagtgga accgcgcctt tgaggccgct ggctggaaag atgcggtgcg
      361 cgcttcgctg ctgccgactg gggtttcgat cgatgatccg cgcgccgctg tgatccactg
      421 ggatgtcggt gagccttcgt ttaacggtcg cggacagcca cttgtcgatc cgcgctcggg
      481 ggaaatcctg ggcgcgtcgc tgattctgtc gatgggaccg gtgcgcacgc accttcggct
      541 gcgcaaaact gtgctcgctc cgcagacaga aaaccagcag agcagaagtg accacgccac
      601 aatcaacgga tcgcttttgg ccgatgaatc cgatctggcc gactcccttt ccgaacaagc
      661 cgaagtgctt cgtgccgcgc ttttgtcgac ggatgcagtt caaagcgatg gcccacttcc
      721 ggaagcgctg attggacagc ggctgcggct tctggcaatg cacgaagccg gtcacgcgct
      781 cggactgcgg cacaactttc gcgccagttc cattgcgccg agtgaccagc ttgggaatcc
      841 cgattggcag cggcagagtc cgttggtttc gtcggtgatg gactatcccg cgatcaacct
      901 gccgcccccc ggatcgccgt ccgccaagcg cggttatgat ccaagcttcc tgtattaccc
      961 gacgagcctt ggtccttccg atctgctcag catcgcttat gcatacaccc cagatccggg
     1021 ctatgccgcg cagcttgcca agcaggccgc cgcctggggt tatgtcttcg gcagcgatca
     1081 ggaagccagc ctgggggccg atccgctggt gcagcgctgg gacttgggtt ctgatccact
     1141 ggcctgggcc aaagaccgca gcgagctttt gcgcaagctg tgggtggaca cgatgaagac
     1201 ccggctaaaa aaaggcgagc gccccgcgga aatcaccgaa cagctgggct ggctgctcgc
     1261 cgattatgcc cgcgcagtcg acgccgcccc gacgtatatc ggaggccgtc acagctcccg
     1321 cgatcacatc ggcgatcccg gtggccggcc tgcgtatcgt ccggttgaaa aagcaaaaca
     1381 gacggaagcg ctcgcgtttt tgctcgacgc ggtgttttca gaacaagcgc tgccgatttc
     1441 agcagagctg gcgcagcaga tgggcgacga ctatctggat gtccgacgac gcgacgcccg
     1501 caaccagacg gtctatccgc tgtggcgaca ggtgctcgat ctgcgggtct cgacgcttca
     1561 cgatcttttg tcaccactgc ggctgcggcg catggtcgat ggcgaacagg tattcggacg
     1621 cgacgcggtg ctcaccgtcg gtgaactgct gggcaagctg agccagtctt tgtttgccga
     1681 aattctgtca ggaaccccgt ccggcctttc gcccatgcgc cgagagctac agctccagta
     1741 cattgagtcg cttgggaatc tcgtgctcca gcacgcacca gagagtcgac atgcgcgatc
     1801 gcaagcgcgt tatcagcttc aggaactgcg cggcaagctc gatgcactgc gcagcaaacg
     1861 cgacagcttc gatgtcgata cgcgggcgca cttggcagaa ctagaggact cgatttcagc
     1921 gacggtaaac ggagcgatgg tcattcgtcc ccctgccgat tcccaaacgc actcccacag
     1981 cgccgaatag ttccccggaa ggcccctttc cgattcactt ccgcagtgtg gcgggccagc
     2041 cgggctggtg ggggaacact cccaagaaat tttttttcgt ttgggagtcc cttcggagcc
     2101 cccctagtgg ggaaaaaatc ccacccccct gatgaatatt ttcccacttt acgcgacggg
     2161 aaaccagcgg ccataactct gttcatgatg gccacgctgc ttctggcgtg acccaccggc
     2221 ccaaagggtc tcctggaccc aaagacagag gaactctcat gaaacgtacc gccaccctcc
     2281 gctttctgtc tcattccgtg ctggctttca gttcgtttgc gctggctgct ggttgttccc
     2341 cggcccagct cgacgaggtt tccactgaag aggaactgct cgccaccgcc gcgaccgtga
     2401 agggaaccac cctgcgcacg ctcggttttg atgcggcttc gcttgatgcg gaaaacaaac
     2461 agccggtgta tttggccggt cgggtgccgg ctgcgatttc tggggcagaa tcggcgcggg
     2521 acttcgcgat gaacctgctg ggccggtcgc tgcgactttc gcccgattcg gacttcgtgt
     2581 ttcacaaaga gacgcgcggc gaagatgggc atcgttatct gcgactgcgc cagacacacg
     2641 gcggcgtgcc ggtggagcaa ggcgagctga ccctgcaagt tgcagaagat ggcgcggtgc
     2701 gttcggtgct cggtcgtccg ctgccgaacc tgcgcatctc gacgcgcccg acgctgctcg
     2761 gtgatgcggc gctgaaaggc gcgctcgcca agcttgtgaa gtcagggaat gcccaggttc
     2821 acaagtcccc cgagctggtg attcacacaa acctcgccgg ccagccagtg ctgacctatc
     2881 acgcggtggt cgaataccag ggcgcatcgg gtcttgcgtt tgaggatctg tatgtcgatg
     2941 caaacagcgg ccaagtggtg tcgcaaattt cgcgcatcca caccggcctg tcgcgcacca
     3001 tttatgatgg gaaaaaagcc tgcttgtcga cgggaagtga gctgcccgga acccttttgt
     3061 ataacgaagg cggaaccagc actgacacgg tggcaaacgc cgcgtacacc aacaccggcc
     3121 tgacgtattc cttttacaaa aacttccatg gccgcgactc ctatgacgac aagggagccc
     3181 cgctcaagtc gtcggtgcac ttcacgttca acagcggctt ttcttgcgat ggcgacaacg
     3241 ccgcatggct gggcgaccca tacaaccaga tggtctatgg cgacggcgat gccacgatgt
     3301 tcaagccgct gtcgctggcc atcgacgtca ctgcgcacga actgacccac gccgtcaccg
     3361 acaagacatc gagcctggtc tattcgaatg aatcgggcgc gctcaacgaa gcgatgtccg
     3421 acatctttgg tgcgattgcg gaagcgtgga aagcgtcggg cggcagcgcc agtggcaatc
     3481 cggcgtcgat cacccccacc gccaacacct ggaaaatcgg cgaagagatc gcccaggcgg
     3541 gtctctcggg cggcgcgctg cgctttatga acaatccgac cgccgatgcg tacagcaaag
     3601 actattaccc cgagcggatc accggcagct cggacaatgg cggcgtccac ggcaacagcg
     3661 gcatcgccaa cctggcgttt tatcttctgt cgcagggcgg cagccatccg cgggccaaga
     3721 ccacgacggt ggtcacgggc atcggcatcg aaaaggccgc caagattttc tatcaggcca
     3781 acacgtcgat cctgacaagc tcggccaact tccaagcggc ccgcaacgcc accgcgcaag
     3841 cggcgcagac cctgtacggt tcgtgctcgg cggaatggac aaacacccac aaggcatggg
     3901 acgcggtcgg cgtcaccggc acctggacgc cgtgcggcac cggagacacg acgccaccga
     3961 cggcaaccct gaccgcaccc agcgccggca gcacgctgac gggcaccgtg accgtttcgg
     4021 cgagcgcaac cgacaacatc ggtgtggcga gcgtcgattt ctacgccggc accaccctga
     4081 ttggcaccga caccaccgcg ccgtactcga tcagctttaa ctcgacgacg gtggcaaacg
     4141 gcagctacac cctgacggca aaagccaagg acggcgccgg caacgtcgga acctcggcag
     4201 gcgtggccgt gaccgtctcg aacagcggcg gcggaaccac ccaaaacgaa gccgagccga
     4261 acggttccta taaccaggcc aacacggtgg cgacctcggg cacgacggtg acgggctaca
     4321 tcacatcgag caccgatgac gactacttca aggtcacgct cggcgcgggc aagaccctgc
     4381 aagccgtgct gaccccgccg tccggttcgg acttcgatgt gtacatctac gaccagtcga
     4441 aagtgctcgt cgccaagagc gaaaacgatg tcggcctggt cgattccgcc agcgcgacca
     4501 acaaggccgc gagcgccggc acgttctgga tctacgtcaa gtactacagc ggcgcgtcga
     4561 agacgaaccc gtacaagctc aagctgacgt tctagtcccc ggcccgaacc ttcgggcccc
     4621 actccccacc tccgcctgtt ttttccccgt ttctcgcacc gccgcttctg tttcaccgct
     4681 gtttggtcgc agcggcggtg tacgttttgc cgcatggcgc aaagtgctgc aaaaacggtg
     4741 aacgaatatc tggccgagct tcccgccgac cgacgcgaag tgatcgcgac cctgcgtgag
     4801 cttatcgtcg caaatctgcc tgcgggttat tccgaagcga tgcagtacgg gatgatcggc
     4861 tacgccgttc cgctttcgcg ccatcccaac acccacaatg ggcagccact cgccgtcgcc
     4921 gcgctggccg cacagaaggg atacatgtcg ctgtacctca tgtcggtcta tggcgacccg
     4981 gcgctctgtg catggtttgt cgcagagcac gagcgcgccg gcaaaaagct cgacatgggc
     5041 aagagctgca tccgttttcg caagctcgag gaacttcacc tgccagccat agccgaagtc
     5101 atccgccgtg tcagcgtcga agacttcatc cgcacctacg aagcggcccg tgcctccgcc
     5161 gcgagtgcca aacgaaaagc caagaccgcg tcttaggcaa gccaagctga accgttttgc
     5221 ggctgcggtt ttgttgtcgt cagagttgac acggcgtgcc gggcagatga cacttccgcg
     5281 gacacatgca ccggttgttt cgcgagctta cgttcacacc attggcactg cgggtgacgc
     5341 tgctcgccgc tgttctgctg agcggaggct gtgccgcttt cgtcgagaac ttccgccccg
     5401 tcatctacgg caaaaacgcc aaggaaaact acgagcgcgg catccgttct ctcaaaggcg
     5461 aaagcagcct cgacgcggcc aagtacttta actacgtgcg ccaaaactgg ccgacctcgc
     5521 gctgggtgcc gtgggccgag ctcggccttg ccgacgctgc gttcgggcgc gaggcgtaca
     5581 tcgaagcgat cgacggctac aagaccttca tcgccagcca cccgcgccac cccaagacgg
     5641 tcgacggata ctgcgcgttc aagattggcg aatcgtactt taagcagatc ccgagcgact
     5701 ttttcatcct gccgccgtcg tatgaaaaag accaaggccc ggtcatcgac gcccagcgag
     5761 agctaaccag cttcctcgac cgctacggcc agagcgccta tgccgaatcg ggacgcaagc
     5821 tgcttgccga tgtcatccag cggctggccg accacgagct gtacgtggcc cgctactaca
     5881 tggaacgggg caagaccaaa gcggcgacct ggcgactgga atacgtggtg cgcgagtatc
     5941 ccaaagcgcg acgggatgcc gaagtgctga tgatgctcgg gcaggtctac ttgaagcaaa
     6001 atgagccgaa ggacgcgcgc gacacttttc gccggctttt gatcgaacat ccggacgact
     6061 atcgcgtcga tcaagccaag ctgttcctcg attacatcga aaagaacttc ggcgatctgc
     6121 caccgcctgc gccgctcccg ccgcgcaaga gcccagggca aaaaggtggt cttttgccac
     6181 ccgacgatag tgatctgctg ccgccggaat agctcgacgc agcaccgccg gcccacagac
     6241 ggcgagcggc ttgaccaaag ccgcgggcgg aaactacact aggacgctta ctaacctccg
     6301 aactgcggtt tgttttcgtg gccaatccag gtaccaaaga gaaactcatc gaagcagcga
     6361 aggaactggc cgaaaaaggt cagttcgaca aagcgctgcg cgaatacctg aaggtcgctg
     6421 ccgaggatga aaacgatgtt ctcgtcttca ttcgcatcgg cgatctctat gtgaagcttg
     6481 gccagaagca agaggccatt gccaactacc agcgcgttgc ccagctctac gtcggcaccg
     6541 gtcagctcga caaggcggtg tcggtctatc agcagatcct gcagctggat ggcaagtcgg
     6601 cggacacata catgacgctc ggcggacttt ttcgcgacca gggcaagttg ccgctcgcga
     6661 tgcagcagtt cgagctggcg gcgcagcttt acatgcgggc cagtcgcacc gcgcagtcgc
     6721 tggcagcgga acaggcggtg gtcgacctgg cccccgacag cctgccgcgt cgcatccgca
     6781 ttgcggagct gtattcgaaa gaaaatctgc cgaccgaagc cgcgcgtgag tttgaaaaag
     6841 ccgcgcagct tttgcgcaag caaggtcggc tcgacgactg ggcgaaagtg atggagcggc
     6901 ttttgttcca cgctcccggt cagatcgcag agtcaaacga gctggcgcgc tactacctgg
     6961 cgcaaggcga tggtctgcgc gcgctgtcac ggctccaggc cagctacaaa gtcaatcggc
     7021 aggatcgcga ccagctcgag ctgctttcgc agaccttttt gcttttgcaa aagccggaca
     7081 aagccgttgc cgtgctgaaa gagcaggccc gtcagacgca agaccgcaag gttgccgagt
     7141 ctgtctatcg caagattctt gaaattgatc cgacggacgc cgatgcccag gttgcgctcg
     7201 gtgggggact gttgccgtcg gccacttcat ctcaaaccaa ctttacttcg gcgctcattc
     7261 tcaactcgcc gagtccagga cctgtgccag cgtcgaaaag ctcgccgctg ccagcatcca
     7321 cggtctcggc gcgtccgacg ccatcggtgg tgcccgcgat gcctgctcgt actagctcag
     7381 acatcacgcc agtggttgcg gtccgtgttg atggcacgct gccgcccatc agccggcctg
     7441 cggtcgagcc gctcagcgat gaagaagaac aggatcgcgt cctttccgag accgattcgt
     7501 ttgtgcgctt cgggctcaaa ggcaaagcgc tggagtcgct gcgcgcgacg ctgagtcaca
     7561 ggccgcatct tgccaaagtg cgggaaaagc tatccaaagt gtacgagctg ctggggcagt
     7621 ttaagcccgc gatcgccgag ctgcgcaccc tggccgatcg cgcggtcagc catgaggagc
     7681 aggcccgtta tctgcgtgag atcctgcggc tcgacaaccg tgacccggcg gcatcggaaa
     7741 aactgcgccg aatcagcggt gagtttaagc ccattgcgcc gcccgctgac gaagacgagc
     7801 ccgtcatcga aatgagcgcg gaagacgacg ggccgacgac gccgaactcg ctgcccaagt
     7861 ccgaaccgca agcgctgcca ccggccccgt ctggtccctc gtcgcgtggt gcccaaacgg
     7921 aaaactcggg atcaaaagcg ctgccgtctc agcgcagcgg caccctgcgg gaagagctgg
     7981 cggaagtcga cttctttctt cagcagcgga tgttcgacga tgccaagcgg cttttgcgtt
     8041 ctttggcgac gcgatatccc cacagtcaga cggtgcaaaa caagctgtcc gagctttcgg
     8101 tcagcgcgtc ttcatccgat gaaaacctga tcgaactgga acccgaggag ctggtatcag
     8161 acagcaatcc caacgtcggc aagaagctga cgggtgtggc tcgtcctccg gaaaagaaag
     8221 ctccgccgtc ggcttcggcg cgcagcatcc cagtgcctcc gtcggtcacg agcagtggtc
     8281 cagcggaaaa aagcggaccc ataagcgaaa cgcgcgaccc agccgaggcg tatcagcgcg
     8341 gcatcgccca ccgcaaccgc ggacagcacg ccctggcgat tggagagttc aaggctgcca
     8401 tgcaagatcg caagctggcg ggccgggcag cgctgatgct cgggcagtgc taccgcgatc
     8461 tgtcgcagct ccgcgaggcg gtcgaggcgt tcaaggaagg tctgtacatg ccgtcggtgg
     8521 ccgacgcaga cctggccgaa atgaactacc agctcgcgca gtgctatgag cggctgcaaa
     8581 accccaaaga agcgatctac ttctaccagc aagcgcagca agcccaagcc ggctacaaag
     8641 acaccgccgc ccgcctgagc accctgcgga agtaatcagt acggaattcc ctgctctttg
     8701 cgaagcgtgg cttcgtcgaa gccgggctcg atgtagatgc gacgcaggtg ggggaacttg
     8761 gcgcgcagct ctgcctctag gtcgtttatc ttggcctcta ggccgtccat cgtcaggtcg
     8821 cgggcaaaac gaatcttgaa cgtcgccagc acttcctgcg gtgcgatgtg cttggtgcgc
     8881 aggtggatga cttgcaagat gccgggatgc gccgctgcga gcgccacgat ctgctcgtgt
     8941 tcttccttcg gcaccgcttc gcctatcagc aggctcatcg aatcgcgccc caaaaacaac
     9001 gccacgacga gcagcgccac accaacgaca atcgacgcga gcgcatcaaa catcaggttg
     9061 ccggtcacgt agcttaggac cacaccgacg agcgcggcga acagaccaaa cagcgccgcc
     9121 atgtcctcga acagcacggt cagaacggtc ggatcgcggg catcttcgac ggtcttgcgc
     9181 aggcctttgc ccgccgtcat gtgcttaaac tcctgcaatg ccgcgcgcag cgaccagcct
     9241 tcgagcacaa gcgacacaga aaggacaatc accgcccact tgatgttgcc gtgggcgatg
     9301 ggctgaccgt gcaggatgtg ccacagcttt tcgctgcctt cccagatgct gaccgcggca
     9361 ccgattagga agatgctgcc ggcgacgatg aatgcccaaa aatacgtctc ggtgccgtaa
     9421 ccaaaaggat ggcgggcatc gggcgggcgc gagctgcgct tcatcccgat gagcaggaag
     9481 acttggttcg cggtatcggc cagcgagtga accgcttcgg ccagcatggc cgtggacccg
     9541 ctgattccgg cggcgacaaa cttaaacacc gcgataagca ggttgatgca aagtgccacc
     9601 agcaccacgc ggagcccgcc accatgactt ggctcgacgg gcggcttcga ttcgctcata
     9661 caattccccc cttacagtgc ggtttcgcta tcgaccaagt gcccagctcc cgacgggctc
     9721 gcgaccgatg acaccacccg cagccacagc tcttcgattc cttccccttg ttcagccgca
     9781 cacaccacgg cgcgcccacc gagcagccga gacagaagct gcgccgctgg cttgcgctcg
     9841 gacttggcga gcttgtcggc cttggtcagc acgggaatca cctggcgatc taggccgcgc
     9901 agatacgtcg atagctcggt ttcatcaaac agcagctctt cgacccgtgg atcaagaacg
     9961 cgacgcgcat cgcagagcag catcaccgcg cgcaggttgc tccgcttttc gaagtactct
    10021 tccatcatct tttgccagct ctggcgctcg ctgcgactga cttcggcata gccatagcct
    10081 ggcaggtcgg ccaaaagcac gctcagcgga tcgcgcggac ctttgagctt ggcggaaaag
    10141 aagttgataa ggcgggtgcg tcccggcgtg cgcgaaaccc tggcgagtcc ccgtctgcca
    10201 agcagtgcat tgagaagcgt tgacttgccg acattgctgc gaccgcagat cgcgacttcg
    10261 gcaaggcttt cagcaggcca actggaacgc gacggggcgc tggcgacaaa atcgctgcga
    10321 aggatttgca tcggacggct cattgtaact aggcttcggg ttctacgcgc ggcttacgac
    10381 aaggctgaca ttgccgccgc cctgggcgat ggtcgggatc agcacatgct cgacggggga
    10441 tggggttggg aagcggacaa gtccgggcag gctgcacgtc ttgtcggggg tccggcaatg
    10501 cgtggtgcca ggcagtgtgg ccgaccgaag cgccgagagc gcagcgccaa accgcaggcc
    10561 cgtgctcatc agcgattcgc cacactggcc caagatcgaa gagatcggaa cccgctcggc
    10621 cacctgacca agcgccgccc gcagcgctgc ggtttcgatg gcgtcgcatg cagtgccgtt
    10681 gcccgcgccc gcgacatacg acacgtcttc gggccgcaga ccggcgctct gcacgctctg
    10741 tacgatgctc tgtgcagcgc cggaaaacag gtgcgcgctc ggtgggtgac tggggtcttt
    10801 gccgtcactc gctgcgacgg tgggccagcc gatccgatgc cggctgtcgc ccgcccgcgc
    10861 ccagcccgac aggtagccca gacaaggcgc ggaggtctcc gcatcggcgc gttccagaag
    10921 cacgatcgcc gcgccttcgc cggggacaat gccaccggaa ccgctctcat agggatgcag
    10981 cggagcgccg tcgccttcgt tcaaaaacgg tgcaagcgcg ccaagtcgcc acagaccatg
    11041 gtaaagccac gggcccagct cgtcgcagcc gccacacaga acggcatcgg cgcggtttgt
    11101 caaaagcagc tcagccccgg tggcgatgga ctcggcaagc gacagctcgc ggtggtttac
    11161 ggtgatgttt ggcccggtca aagcaagctc gatggcgaca agccccgccg acgcattcat
    11221 cacggtatac ggaaagaccg ctggggacgc cgcagcaaga ccaccggaca gaaagctgcg
    11281 aacaaaatct atcgtgtact ccacgcagcc aagcccggtg cccagcacga cgcccagtcg
    11341 atcgggacga tagcgccgat acagcgcggc atcttggctc gcagcgccgg gctgtagacc
    11401 catcgcttcg cgcgcggcga cgatcgccat ttgggaaagg cgcggcatgc gtcgtcgttt
    11461 gccagcctca atggctttgt cggcaccaaa atcgggcacg cgaccggccc acaccggctg
    11521 tctggccaaa tcacccggca gcgtactgct caattcaggg tgcagcgcaa gcgcactacg
    11581 cccatcagcg agcgcagaaa agaagctcgt ctggcctgcc ccaaacggcg agaccagccc
    11641 aaggccggtg atcgccacct tcggcagaag cgggacgcta gacatggcgc tatttgctat
    11701 catgcaagcc atggtgaaaa cgaaaaaa
//
LOCUS       DAORGN010000158        11654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2302_length_11654_cov_5.592206, whole genome shotgun sequence.
ACCESSION   DAORGN010000158 DAORGN010000000
VERSION     DAORGN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11654
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_11654_cov_5.592206"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="PKL17_15710"
     CDS             complement(<1..1614)
                     /locus_tag="PKL17_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46232.1"
                     /translation="MTDVTRSSSKISSRLGLVAAAVAGLAFYAGCPDPSTTTDGGVTK
                     DFAGTVVDMAQNPDGGPVVQPVGCSVSAWCWQFPVPQGNGLSATWGSSASDVWAVGGA
                     GTIIHYDGSKWSMVRSPTNNALYAIWGSSANNVFAVGDRGTVLRFDGSTWSQISLGTD
                     GAQVAQLYGVWGFDSSEVWFVGNHLTAGAALLKWDGSQVTRLTPPSGVSSGLRGIWGT
                     SKSSVYAAGETGTWLKWDGTAWTKFGPVMSASWSAITGTSESSIFLASHLGLIYRWNG
                     TALVNVPIASSTSINSLWSDGTSVFAGGDIRYPTLGDPTKREGMLLKWDGTTFSRITN
                     TPKVHIYGLWGATGGKVFFAGAAGTMGSFDGTTFVTNSVLEDLTGNNGAIYGLSIGSQ
                     GDVIAVGDSGGTLINKTGSFTPSPGPTGLRIYSVTQLGSETLAVGFDFSQGKGVALKW
                     NGTGWTPETVPTVDRLVKIASDGTNAYAVGDNKVFLVRSGGTWKQVAVPLLEQTTLRD
                     IAALDSTHVWIVGGGDKTETNLGPQALFYN"
     gene            1739..2491
                     /locus_tag="PKL17_15715"
     CDS             1739..2491
                     /locus_tag="PKL17_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNK46233.1"
                     /translation="MNSATKQTYKLLIFDLDGTLIDSVPDLTTALGLLLQSLSLPPIT
                     ETVVRQIIGEGQRSLVERALRRGGHPDAQGETVPPLLVDDSVKRFRGHYSANLCVKTR
                     LYDGVTTTLRELGNTATLAVATNKPGRWARALCETLGIAPLFRFVLGEDDVGARKPDP
                     KMLLDLCERMGVQPKDVLFVGDSRIDWRAAEAAGCDFALCTYGYGDEATFAQGQALAT
                     DGQSPFRLGTAQRPFLCRQFPDLLTVAGLPNA"
     gene            2701..4254
                     /locus_tag="PKL17_15720"
     CDS             2701..4254
                     /locus_tag="PKL17_15720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46234.1"
                     /translation="MTEKMPKPDDVVFGYRIVRVIGKGGMGAVFEGVDDKTGQRAAIK
                     VLHAQLSQDPENLARFLNEGKAIAALNHPSIVRMLGSGQMDGGGCFIAMEYVDGETLS
                     AKLKREKRLGRLGLRLGSEIASALQAAHRRNIIHRDLKPSNVLIKASDGGDPEIRIID
                     FGIAKMAAEDREQDFHTRTGTMIGTPVYMAPEQCRGVTVTDRTDVYALGVILYQMLTG
                     RPPFYSQADGDILAMHILVPPKPLREVEPAIPENVAAFVERMLAKEGAERPSMAEVEM
                     TLRELADDPGWSGKAAITDDTAVSPTPIQPDTAESLPTQVSHVLGAPTLGAINGQAAP
                     PTKKSGLRYGLALSVAALVIVSSVVLAMQLRHKTPQPVENHPVARVEVTALPKTVHWL
                     VKTTPVGATVFDEDGKELGQTPWELSQPLAHGEQVLIVRKAGFVDLRMSLAKDRDIER
                     SEALVSAASPTLTKPVAATKPATVTKPATATKPPPGGETRPAKQPGNTKVRKNDGLIF
                     DFKTRKKLN"
     gene            4265..5065
                     /locus_tag="PKL17_15725"
     CDS             4265..5065
                     /locus_tag="PKL17_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46235.1"
                     /translation="MALLLLAGHRAEAADAFAKRVTKAQNCYERKLFDCAINELEAAY
                     LIRPMPALLLNIAHAYLDYGRPRDAIVYYDRYMAEEKNLAPEARADVEKFKKQAEEKL
                     VAMQGTPPPPPIEKPVEKPEEKPPEEKPPEVTPPKVDTGSQPSAAAKWAPIGAMAIGG
                     ALLISGFACGGAAMSTAKTVVATDGTFNSELDTKGKSLSNAAIALDVLGGVAIVGGLI
                     PTIIRLTKKAPSTVEKATATDGAAPPPAAAPQVSFSFSSRGLVVLGRF"
     gene            5065..5967
                     /locus_tag="PKL17_15730"
     CDS             5065..5967
                     /locus_tag="PKL17_15730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNK46236.1"
                     /translation="MRRTLVFALAGLAACYNPDYSQKPCQDNSGCPTNYYCSAEKRCT
                     IGMGTNNPSADLAPDTRPTRIEVLVSQTGSFWMGTGATDGINDTPNYLRSIPVAYYLD
                     EREVTVADYRACVTSQNCTAPATGPFGTQGWVCTYGVSGKDNHPVSCVTKTQAETFCK
                     WMGRRLPTEQEWEFAALGTLGKDTGQRMVFPGPLGNNACWNQATTGTCPTASYPTFKT
                     YLGQIVSTDQPGFYDLLGNVWEKTSSQFCTYPNETCTTDKYTLRGGSAFDSDLRFSKA
                     TARYPTATTEEYYPNQGFRCARSM"
     gene            complement(5928..7187)
                     /locus_tag="PKL17_15735"
     CDS             complement(5928..7187)
                     /locus_tag="PKL17_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNK46237.1"
                     /translation="MPRRHRLAIVCSHPIQYLAPWFVQLAQSPRLDVTVLYGSLEGQV
                     QAAQDRDFGRALTWDVDLLSGYRYVELHSHANKPGLDRFWGVLSFDLVQHLRRDRFDA
                     VLILGWNYALYPLALSTAVAHRLPVILRGDSVRYRDADENEATLRGLARLQLLSKQQI
                     LRRYVGFCAATLAVSTGNRRLLTHYGVPDDKIFFAPYAVDHARFRLPTVERDALRRTL
                     RQQLGALGDRPVLLFCGKFSEVKAPQLLLSAYRRLRQSGIDASLWLCGDGALRDSLVQ
                     DVARGAIPDVQFLGFRNQRELPGLYAAADVLVVPSQREAFAMVVPEAMHAGLPVIASD
                     RVGCVEDLVIPEQTGLVFPAGDEDSLLRCLSSLCAGESGKLRRERLGQAANQNMATWT
                     YAETTQGLLAALDAIYIDRAQRKPWFG"
     gene            complement(7197..8594)
                     /locus_tag="PKL17_15740"
     CDS             complement(7197..8594)
                     /locus_tag="PKL17_15740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46238.1"
                     /translation="MSKQPNPPQGQGTKPAPGPSVELPSLAIASTVGPSGIPVRPHIG
                     ASPAAEVDKTVGVDQLGLIDTDRGEGPDSTKQDVVTTAKDVRRETTVLPLVEMRGEKV
                     ELVRKSNARYEKIKELGKGGMGEVSLVRDNDIGRIVALKRLYAPPSGDQSGLVRFISE
                     VRTVGQLEHPNIVPIHDVGLDENGNYYFVMKYVDGETLEHIIKKLADGDPYYHQRYPV
                     DVRLEIFMGLLRALQYAHDQGIVHRDIKPANVMIGRYGEVVLMDFGVAKQIRGSGDKL
                     PEKATEPVKPDAQARLFATRNDQLIGTPAYMSPEQAMGRNDLIDERSDIYSAAVLLHE
                     LLSLRHYLKEVTSLNGVLMAIATEPFSYWQLIFVRHPSHPVPRSELLHFVAKGLHKDP
                     NQRFQSVIEMIGELQRLIDGRCAVLCPATLAKRMTREVTGFIERFPKLSPFVFYPALL
                     FLFYSVAFTIYRTVL"
     gene            8771..9649
                     /locus_tag="PKL17_15745"
     CDS             8771..9649
                     /locus_tag="PKL17_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK46239.1"
                     /translation="MSRRVSLSTGLRYHFLEWGGDDQQIDHTVVLVHGFLDLSWTWEA
                     VVAAGLAGKYHVVAPDLRGHGDSDRVGPGGYYHFMDYLADLEDFIAKVGRGRVSLVGH
                     SMGGSVSAYFTGVYPQRISKLALLEGLGPPDNDWSGWPDRLSAWISAWKRQSERGSSK
                     RYATIEEAAARLRATDPKLGPELALRLAEVSTVRTPDGRFQFKHDPLHLTPAPSLFMV
                     DGATTLWRRIACETLLIDGQDSEFRYSPEDATRRRAAIPNARHVELAEAGHMMQRHQP
                     ALLAQILDEFLADPAR"
     gene            9785..10396
                     /locus_tag="PKL17_15750"
     CDS             9785..10396
                     /locus_tag="PKL17_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46240.1"
                     /translation="MTSASLFEKTWDVAIKLCRGGQLDGQHFVVRLADDAPQGALRFR
                     ILNRQGQAVFSPPPDDETEAGGLFTAARRFVELEESESRGIEPPDFVPLARRTYKDSL
                     HEWGLMDGEGRVLKAYEYDPEPLLGYVVRVNDVYAYGIVKQTALAEGTITPTGACDGP
                     DEARRRAIELLYLAMVRGATVMGMNGVPAVIDLSADPEDDDNP"
     gene            10502..10574
                     /locus_tag="PKL17_15755"
     tRNA            10502..10574
                     /locus_tag="PKL17_15755"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10535..10537,aa:Ala,seq:ggc)
     gene            complement(10651..>11654)
                     /locus_tag="PKL17_15760"
     CDS             complement(10651..>11654)
                     /locus_tag="PKL17_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNK46241.1"
                     /translation="TRDHAYRMAEQVVSIAMQDGQLSVRQALDAYRTHQTEKGNRPGS
                     VHNTGQVLLRFFGPVLESPLHKVTPQKGRDLYDRLCRERSSATGRVVATDTHRNYLAQ
                     AKTFLTWCIGQRWIRIHPLEAVSGIGKRRHGKPQLTIDEARSLYKVCFQVADRDDGAL
                     AVLLALLLGLRASEITSRTVRDIDDGGRVLRVGSNAAIEFDPKTRAGKRAIVIPEVLR
                     PLLKLRTRHKLPAALLFPTEEGGPHWRDWVAEQTRRFCRQAGLPVVCAHSLRGVAAST
                     AVAAGVAAEAVAKTLGHESTEITRRAYIAPGTLERAERDQVCAVLAGSSMSQSLGQT"
BASE COUNT         2357 a         3749 c         3446 g         2102 t
ORIGIN      
        1 gttatagaac agcgcctgcg gtcccaggtt cgtttcggtc ttgtcaccgc caccgacgat
       61 ccaaacatgc gtcgagtcga gcgccgcaat gtcacgcagc gtcgtctgtt ccaaaagcgg
      121 aaccgcgacc tgtttccagg tgccgccgct gcgaaccaaa aagactttgt tgtcaccgac
      181 ggcgtaggca ttggttccgt cgctggcaat cttcaccaga cggtcgaccg tcggcacggt
      241 ttctggggtc cagcccgtgc cgttccactt gagcgcgacg cccttgccct gcgaaaagtc
      301 gaacccgacg gcaagcgtct cgctgccaag ctgcgtcacc gagtagattc gcaggcccgt
      361 cggaccgggg cttggggtaa agctgccggt cttgtttatc agcgttccgc ccgaatctcc
      421 gacggcgatc acatcacctt ggctgccaat cgacaagccg tagatcgcgc cgttgttgcc
      481 ggtcagatcc tctagcacgc tgtttgtgac aaacgtggtg ccgtcgaacg atcccatcgt
      541 accggctgcg ccagcaaaaa agaccttgcc gcccgtcgcg ccccacagac cgtagatgtg
      601 gactttcggg gtgttggtga tgcgcgaaaa cgtcgtgccg tcccacttga gcagcatgcc
      661 ttcccgcttg gtcggatcgc ccagcgtcgg gtaacgaatg tcgccgccgg caaacaccga
      721 cgttccgtcg ctccacagcg agttaatcga cgtcgaagag gcgatgggga cgttgacgag
      781 cgcggtgccg ttccagcgat agataagacc caagtggctg gcgaggaaga tgctgctttc
      841 gctggtgccc gtgatggcgc tccacgacgc agacatcacc ggaccgaact tggtccacgc
      901 ggtgccgtcc cacttgagcc aggtgccggt ctcgcccgcg gcatagacgc tggacttgct
      961 ggtgccccag atgccgcgca gaccgcttga tacacccgat ggtggagtca ggcgcgttac
     1021 ctggctgcca tcccacttga gcagcgccgc gccggcggtc aggtgattgc cgacgaacca
     1081 gacttcggaa gaatcaaagc cccacacgcc gtagagctgc gcgacctgtg cgccgtcggt
     1141 tcccagcgaa atctgcgacc aggttgatcc gtcaaaacgc agcaccgtcc cgcgatcacc
     1201 gacggcaaag acgttgtttg cggacgaacc ccagatcgcg tacagcgcat tgttcgtcgg
     1261 cgaacggacc atcgaccact tgctgccatc gtagtgaatg atggtgccgg cgccaccgac
     1321 ggcccagaca tccgaagcgc tcgatcccca ggtggccgac aggccgttgc cttgcggcac
     1381 cgggaactgc cagcaccacg cgctgacgct gcatccgact ggctggacga ctggcccacc
     1441 gtcgggattt tgcgccatgt cgacgaccgt tccggcaaag tctttggtta cgccaccgtc
     1501 cgtcgttgtg ctcggatcag ggcaacccgc gtaaaaagcc aaaccagcga cggcggcggc
     1561 aacaagcccc agccgagaag aaatcttcga tgagctgcgt gttacgtcgg tcattccggc
     1621 ttcctcacgt tgtgaaccta ggaggcaatt gagaaccagt aagcgcgatt gagcttttat
     1681 cttatcgtgt gggaatctct gcggcgccaa tttttgcgaa agtggcccga gcgaactcat
     1741 gaattccgcg acgaagcaaa cctacaaact gctgatcttt gacctggatg gaacacttat
     1801 cgactcggtg ccggacttga cgacggcact ggggctgctt ttgcagtcgc tttcgcttcc
     1861 gcccatcacg gaaacggtgg ttcggcagat catcggcgaa ggacagcgca gcttggtcga
     1921 gcgagcgctc cgtcgcggcg gccatcccga tgcgcaaggt gagacggttc cgccgctttt
     1981 ggtcgacgat tcggtcaagc gcttccgcgg ccactacagc gccaacctgt gcgtcaaaac
     2041 ccggctctat gacggggtga ccacaacgct ccgcgagctg ggcaacaccg cgacgctggc
     2101 cgtggcgacg aacaaacccg gacgctgggc ccgcgccctg tgcgaaacgc tgggaattgc
     2161 gccgctgttt cgttttgtgc tcggggaaga tgacgtcgga gcgcgcaagc cagatcccaa
     2221 aatgttgctg gatctgtgcg agcgcatggg cgtgcagccc aaagacgtac tgttcgtcgg
     2281 tgacagccgc atcgactggc gagccgccga agccgcaggc tgtgactttg cgctgtgcac
     2341 ctatgggtac ggcgatgaag ccacgtttgc gcaaggccaa gcgcttgcca ccgacggtca
     2401 aagtccgttc cgcttgggaa ccgcgcagcg cccttttctg tgtcgacagt ttcccgacct
     2461 gctcacggtg gccggcttgc caaatgcgta gctagctttc cagcttcgca cgcacgcctt
     2521 gcaggccaag cacgcgattt ggccccatat cttgtccggt ttgatccgtc cgcagcgcac
     2581 cggcccggac cgaggacgca tgcgcaaagc tttcacgcaa aggcgcgccc gtggtatttt
     2641 ttgagcttaa gctcgcgacg ataagcgagt cctagagata aggtcgcgtc tggttcttcg
     2701 atgaccgaga agatgcccaa acctgatgac gtcgtcttcg gctaccgcat cgtgcgggtc
     2761 atcggcaagg gcggcatggg cgcggtcttt gaaggcgtcg acgacaaaac tggccagcgc
     2821 gccgccatca aggtgctgca cgcgcagctg tcgcaagacc ccgaaaacct cgcgcgcttt
     2881 ctcaacgagg gcaaggccat cgcggcgctc aaccacccga gcattgtccg catgctcggc
     2941 agcggacaga tggatggcgg cggctgtttc atcgcgatgg aatacgtcga cggtgaaacg
     3001 ctatcggcca agctaaaacg cgaaaaacgg ctgggtcggc tgggtctgcg cctcggcagc
     3061 gagatcgcat cggcattgca agcggcgcac cgtcgcaaca tcatccaccg cgatctgaaa
     3121 ccgtcgaacg tgcttatcaa agccagcgac ggcggagatc ccgaaatccg catcatcgac
     3181 ttcggcattg ccaaaatggc cgccgaggat cgcgaacagg actttcacac ccgcaccggg
     3241 acgatgatag gcacgccggt ctacatggcg ccggagcagt gccgcggcgt caccgtcaca
     3301 gatcgcaccg atgtgtatgc gcttggcgtg atcctgtacc agatgctgac cggcaggccg
     3361 ccgttttact cgcaggccga cggggacatc ttggcgatgc acatcttggt gccgcccaag
     3421 ccgctgcgcg aagtcgagcc agcgattccc gaaaacgtcg cagcgttcgt cgaacggatg
     3481 ctcgccaaag aaggggccga acgtccgtcg atggctgaag tcgaaatgac gctgcgcgag
     3541 ctggccgacg atcccggctg gtcaggcaaa gccgccatca cggatgacac agccgtgtca
     3601 ccgacaccga ttcagccaga caccgctgag tcactgccca cgcaggtcag ccacgttctc
     3661 ggcgcaccga cgctgggtgc catcaatgga caggcagcac cgccgaccaa aaaaagcggc
     3721 ctgcgctatg gcctggccct ttcggtagcc gcgctggtga tcgtttcttc tgtggtgctg
     3781 gcaatgcagc ttcgccacaa aacgccgcag ccggtcgaaa atcacccggt ggcccgcgtc
     3841 gaagtgacgg cgctgccgaa gacggtgcac tggctcgtga agaccacgcc ggtcggtgcg
     3901 accgttttcg acgaagatgg caaagagctg ggacagacgc cctgggagct gagtcagccg
     3961 ctcgcgcatg gggaacaggt gttgatcgtt cgcaaagccg gctttgtcga tctgcgcatg
     4021 agcctggcca aagaccgcga catcgagcgc agcgaagcac tggtttcggc ggcaagtccg
     4081 acgctgacca agccagtggc agcgaccaaa ccagcgacgg tgaccaagcc agcgaccgcg
     4141 accaaaccac caccaggcgg cgagacacgt ccagcgaagc agccggggaa taccaaggtt
     4201 cgcaaaaatg acggcctcat ctttgatttc aagacgcgca aaaagctcaa ctagtgccat
     4261 cgcggtggca cttttgctgc tcgctgggca cagagccgag gcagcggacg cctttgccaa
     4321 gcgggtcacc aaagcgcaaa actgctacga gcgaaagctc ttcgactgcg cgataaacga
     4381 actggaagcg gcgtatctga ttcgtccgat gccggcgctc ctgctcaaca tcgcgcatgc
     4441 gtacctcgac tatgggcgac cgcgcgacgc gattgtctat tacgaccgct acatggcaga
     4501 ggaaaaaaac ctcgcgccgg aagcacgcgc cgacgtcgaa aagttcaaaa aacaggccga
     4561 agaaaagctc gtggcgatgc aaggcacccc gccgccgccg ccgattgaaa agccggtcga
     4621 aaaacccgaa gaaaagccgc cggaagaaaa accgcccgaa gtcacaccgc ccaaggtgga
     4681 caccggcagt cagccgtcgg cagccgcgaa atgggccccg attggtgcga tggccatagg
     4741 tggagcgctg ctgatcagcg gttttgcctg cggtggagcc gccatgtcga cggcgaaaac
     4801 agtggtcgcg accgatggca ccttcaattc ggaactcgac accaagggca agtcgctcag
     4861 caatgcggcg attgcgctgg atgtgctggg tggcgtcgcg attgtcggcg gactgattcc
     4921 gaccatcatt cggctgacca aaaaagcgcc ttcgacggtc gaaaaagcga cggcaaccga
     4981 cggagccgcc ccgccacccg ccgccgctcc gcaggtgtcg ttttcgtttt ccagtcgtgg
     5041 ccttgtggtc ttggggaggt tctaatgcgt cgcacgcttg tcttcgcctt ggctggcctg
     5101 gccgcctgtt ataaccccga ctattcgcaa aagccgtgtc aggacaactc cggctgtccg
     5161 acgaactact actgctcggc ggaaaaacgc tgcacgatcg ggatgggaac caacaaccca
     5221 agcgccgacc tggcacccga cacccgtcct acgcgcatcg aagtgctggt gtcgcagacc
     5281 ggatcgtttt ggatgggaac cggggccacc gatggcatca acgacacgcc gaactatctg
     5341 cgcagcattc cggtggccta ctacctcgat gaacgcgaag tcaccgtcgc ggactaccgc
     5401 gcctgtgtca cctcgcaaaa ctgtacggcc ccagcgacgg gtccgtttgg tacgcagggc
     5461 tgggtctgta cctatggcgt gtccggcaaa gacaaccacc cggtaagctg cgtgaccaag
     5521 acccaggcgg aaaccttctg caagtggatg ggacgccgcc taccgacgga acaggagtgg
     5581 gagtttgccg cgctcggcac gctcggcaaa gacaccggcc agcgcatggt gtttccggga
     5641 ccgctcggca acaacgcatg ctggaatcaa gccacgacgg gcacttgtcc gacggcgagc
     5701 tacccgacgt tcaagaccta tctggggcag atcgtatcga ccgatcagcc gggcttttac
     5761 gacctgctcg gcaacgtctg ggaaaagaca tcgagccagt tttgcaccta tccaaacgag
     5821 acctgcacca ccgacaaata cacgctgcgc ggcggcagtg cgtttgacag tgacctgcgc
     5881 ttctcgaaag ccaccgctcg ttacccaaca gcgaccaccg aggagtacta tccaaaccaa
     5941 ggcttccgct gcgcgcgatc gatgtaaatc gcgtcgagcg ccgcaagcag cccctgcgtc
     6001 gtttcggcat acgtccacgt cgccatgttt tgattcgccg cctgacccag tctttcccgg
     6061 cgtagctttc ccgattcccc agcacacagc gacgacaaac agcgcagcag ggaatcttcg
     6121 tcgccggctg gaaagacaag tccggtttgc tctggaatga ccaaatcttc gacgcagccg
     6181 acacgatcag aggcgatcac cggcagaccg gcgtgcatcg cttcgggcac gaccatcgca
     6241 aacgcttcgc gctgagacgg aacgaccagc acatcggctg cggcataaag acccggcagc
     6301 tcgcgctggt tgcgaaagcc caaaaactgc acatcgggaa tcgccccgcg cgccacgtcc
     6361 tgcacaagcg agtcgcgcag cgcaccgtcg ccacacagcc acagcgacgc atcgattccc
     6421 gactgccgga gccgtcgata ggccgacagc agaagctgag gcgctttcac ctcgctgaac
     6481 ttgccgcaaa acagcagcac cggacgatcc cccaaagccc caagctgctg ccgcagggtg
     6541 cgccgcagcg cgtcccgctc gacggtgggc agacgaaagc gcgcgtggtc gaccgcatac
     6601 ggcgcaaaga agattttgtc gtcgggcacc ccgtaatggg tcagaagccg ccgattcccc
     6661 gtcgacaccg ccagcgtcgc cgcacaaaaa cccacatagc ggcgcaaaat ctgctgtttt
     6721 gacaaaagct gaagcctcgc caaaccgcgc agggtggctt cgttttcgtc cgcatcgcga
     6781 tagcgcaccg agtccccgcg caaaatcacc ggcagtcgat gggccaccgc cgtcgaaagc
     6841 gccagcggat acagcgcgta gttccagccc aagatcagca ccgcatcgaa acgatcccgt
     6901 cgcagatgct gcacaagatc gaagctgagc acgccccaaa aacgatcgag tcctggcttg
     6961 ttcgcatggc tgtgcagctc gacgtagcga tagcctgaca gcagatcgac atcccaggtc
     7021 agcgcccgac caaaatcgcg atcttgcgcc gcctgcacct gaccctctag cgatccatac
     7081 agcaccgtca cgtcgagccg cggcgactgc gcaagctgca caaaccacgg cgccaggtac
     7141 tgaatgggat gtgaacagac gatcgcgagc cggtgccgtc gcggcatcgc cgcaccctac
     7201 agcaccgtgc ggtaaatcgt aaaggcgacc gaataaaaca aaaacaacag cgccggataa
     7261 aacacaaacg gtgacagctt cgggaatcgc tcgataaagc cggtcacttc gcgcgtcatc
     7321 cgcttggcca gcgtcgccgg acagagcacc gcgcagcgtc catcgatcag ccgctggagc
     7381 tcgccgatca tctcgatcac gctctggaag cgctggttgg gatctttgtg cagccctttg
     7441 gcgacgaagt gcagaagctc cgagcgcggc accggatggc tgggatggcg tacaaaaatc
     7501 agctgccagt acgaaaacgg ctcggtcgca atcgccatca gcacaccgtt tagcgacgtg
     7561 acttccttga ggtagtggcg caaagacaga agctcgtgca acagcaccgc cgcgctgtag
     7621 atgtcgctcc gctcgtcaat caggtcgttg cgtcccatcg cctgctcggg cgacatgtac
     7681 gccggggtgc cgatcagctg gtcgtttctc gtcgcaaaca gccgtgcttg cgcatcgggt
     7741 ttgaccggct cggtcgcttt ttcgggcagc ttgtcaccgc tgccgcgaat ctgcttggcg
     7801 acgccaaagt ccatcagcac aacctcgcca tagcgcccga tcatcacatt tgctggctta
     7861 atgtcgcgat ggacaattcc ctggtcgtgg gcgtactgga gcgcgcgcag gaggcccata
     7921 aaaatttcca gccgcacgtc gacgggataa cgctggtgat agtacggatc gccgtcggcc
     7981 agctttttga tgatgtgctc cagcgtctcg ccatcgacgt atttcatcac gaaatagtag
     8041 ttgccgtttt cgtcgagccc gacgtcgtga atcggcacga tgttgggatg ctcaagctgg
     8101 ccgaccgtgc gaacttcact gatgaaacgg accaggcccg actgatcccc cgacggtggc
     8161 gcatacagcc gcttcagcgc gacgatccgc ccaatgtcat tgtcccgcac gagcgacact
     8221 tcgcccatgc cgcctttgcc cagctctttg atcttttcgt aacgagcgtt cgacttgcgg
     8281 accagttcaa ctttttcccc gcgcatctcg acgagaggca gcaccgtcgt ttcacgccgc
     8341 acgtctttgg ccgtcgtcac cacgtcttgt ttggtcgaat cgggcccctc cccgcgatcg
     8401 gtatcgatca gcccaagctg atcgacgccc accgtcttgt cgacttcggc agctggcgac
     8461 gccccgatgt gcggacgcac cggaattccc gacggaccga ccgtcgacgc aatcgccaaa
     8521 gaaggcagct caaccgatgg tcccggcgct ggctttgtcc cctgcccttg cggcggattc
     8581 ggctgcttgc tcatcgccgt atcctgtccg cgccccgagc ttggtcacat ggggcaacaa
     8641 ctgaaaagat cgcgcacatt ttttaacttt acctcagacc gagcgagcac tgtttttgac
     8701 tcggaaactg gcgcacgcag cttgcccaaa acgacctttc tccggcacgc tacagcgcat
     8761 gtctttgccc gtgtctcgtc gtgtttcgct ttcgaccggc ctgcgctacc actttttgga
     8821 atggggcggc gatgaccagc agatcgatca caccgtcgtt cttgtgcacg gatttctcga
     8881 tctgtcgtgg acgtgggaag cggtcgtcgc ggctgggctt gcaggcaaat accacgtcgt
     8941 cgccccggac ctgcgcggcc atggcgacag tgatcgcgtc ggccccggcg gctactacca
     9001 ctttatggat tacctcgccg accttgaaga cttcatcgcg aaggtcggtc gtggccgtgt
     9061 atctctcgtt ggacattcga tgggcggatc ggtgtcggcg tactttaccg gcgtctatcc
     9121 acagcgcatt tcaaagctcg cgctgctcga aggcctggga ccgcccgaca acgactggtc
     9181 cggctggcct gatcggctca gcgcgtggat ttcagcctgg aagcgtcaga gcgagcgagg
     9241 cagcagcaaa cgctacgcga cgatcgaaga agctgccgca cgactgcgcg ccaccgatcc
     9301 caagctgggc cccgagcttg cgctgcggct ggccgaagtg tcgacggtgc gcacgcccga
     9361 tggccgcttt cagttcaagc atgacccgct gcacctgacg cccgcccctt cgctgtttat
     9421 ggtcgacgga gcgacgacgc tgtggcgacg gatcgcgtgt gaaacgctgc tcatcgacgg
     9481 tcaagatagc gaatttcgct attcccctga agatgcgacg cgccgacgag ctgcaatacc
     9541 caacgccaga cacgtcgagc tggccgaagc cggacacatg atgcagcgtc accagcccgc
     9601 actcttggcc cagatactcg acgaatttct ggccgatcct gcgcgataaa acacggcttg
     9661 gcaccaaagc atccgtcgct gcgcggcttc tttccgcggg ggctgcaatt tgtgcgaaga
     9721 tactgaaacc tatgccgtag cccatgactt gggcgaaaac caaacgcata taggacgtga
     9781 gcgcttgacc tccgcaagcc tgtttgaaaa gacctgggat gttgccatca agttgtgccg
     9841 aggcggccag ctcgatggtc agcacttcgt tgtccgactc gctgacgatg ccccgcaagg
     9901 cgcactgcgt tttcggattc tgaaccgtca aggccaggcg gtgttttcgc ccccgcctga
     9961 cgacgagacg gaagccggcg ggctgttcac tgccgcgcgt cgcttcgtcg agctggaaga
    10021 atccgaaagc cgaggcatcg agccgccaga cttcgtcccg ctcgcccgcc gcacctacaa
    10081 agacagcctg cacgagtggg ggcttatgga cggtgaaggc cgcgtgctaa aggcctacga
    10141 atacgacccc gagccgctgc ttggttacgt cgtgcgcgtc aacgatgtct acgcttacgg
    10201 catcgtcaaa cagacagcgc tggccgaagg caccatcacc ccaaccggcg cctgcgatgg
    10261 tccggatgaa gcgcgccgac gagcgatcga gctgctctac ctggcgatgg tgcgcggcgc
    10321 gaccgtcatg ggcatgaacg gcgtccccgc ggtcatcgac ctgtcagccg accccgaaga
    10381 cgacgacaac ccgtagtcgc agccggatcg cccaagcatc tccgtctggg aaccaaattt
    10441 cccaaaattc ctgcttgacc acggcgcaaa acccgactac tacattgctt cgcaagtcgt
    10501 tggggctgta gctcaactgg tagagcgctt gaatggcatt caagaggtaa cgagttcgac
    10561 cctcgtcagc tccacatcaa accccgtcgg tgagtcatct cccgccgggg tttttttatg
    10621 ccctgggaca cgcttgggac tggcttggga ctaggtttgt cccaagcttt gggacatgga
    10681 tgagcccgcc aagacggcgc acacctgatc gcgttcggcc cgctccagcg ttccgggtgc
    10741 gatgtaggcg cggcgggtga tctcggtaga ctcatggccc agcgtcttcg ccacggcttc
    10801 ggcggcgact ccagcggcca cggcggtgga ggcggcaact cctcgcaggc tatgcgcgca
    10861 cacgactggg agtcccgctt gccgacaaaa ccgccgggtc tgctcggcga cccaatcccg
    10921 ccagtggggt ccgccctcct cggtgggaaa gaggagcgca gcgggcagct tgtgccgggt
    10981 gcggagcttc agcaagggcc gcagcacctc ggggatcacg atcgctcgct tgcctgcgcg
    11041 cgtcttcggg tcgaactcga tcgctgcgtt cgatcccacc cgcaacaccc ggcccccatc
    11101 gtcgatgtcg cgtaccgtgc ggctcgtgat ctcgctcgcc ctcagcccca gcagcagtgc
    11161 cagcagcacc gccaacgcgc catcatcgcg atcggcaacc tggaagcaca ccttatatag
    11221 ggatcgtgcc tcgtcgatcg taagctgcgg cttgccgtgt cggcgcttcc cgatgccgct
    11281 tacggcttcg agcggatgga tccgtatcca tctctgcccg atgcaccaag tcaggaacgt
    11341 cttggcctgg gcgaggtagt ttcgatgcgt gtcggtggca accactcgcc cggtagcgct
    11401 cgaccgttcg cggcacaggc gatcgtacag gtcgcgtccc ttctgcggcg tgaccttatg
    11461 gagcggggat tccagcactg gaccaaagaa gcggagcagc acctgccccg tgttatggac
    11521 cgatccgggc cggttcccct tctccgtctg gtgcgtgcgg tacgcatcga gcgcctggcg
    11581 gacgctcagt tgcccgtctt gcatggcgat cgagacgacc tgctccgcca tccggtaggc
    11641 gtggtcccgc gtgg
//
LOCUS       DAORGN010000159        11642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2307_length_11642_cov_5.251402, whole genome shotgun sequence.
ACCESSION   DAORGN010000159 DAORGN010000000
VERSION     DAORGN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_11642_cov_5.251402"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKL17_15765"
     CDS             complement(<1..999)
                     /locus_tag="PKL17_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK46242.1"
                     /translation="MIDFELSEDQKALQKTVREFAQAEIAPHSRKWDNESHFPIELVP
                     KLGALGLLGMTIPEAYGGSAMTMQEVALVIEELAAVDGSVAITVASHNGLCSGHISLF
                     GNEDQKSRYLPDLASGTKLGAWGLTEPGSGSDAAGAKTRATRLADGSWSISGQKTFIT
                     QGSVGGVAVILAVTSPEKKQRGITAFIVDYGTPGFSASRRIEKMGLHASDTAELLLED
                     VRVPDSQRLGELDHGFIDTLRILDKGRITIGAMALGLGAGATAAAIRYAKERRQFGKS
                     LAEFQAIQNMIAQAAMELDAARLLLQRAAWMHDQGMKFGLQASMAKLYAAQAAMRAC"
     gene            complement(1035..1481)
                     /locus_tag="PKL17_15770"
     CDS             complement(1035..1481)
                     /locus_tag="PKL17_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNK46243.1"
                     /translation="MQADTSFGSAKQKLRILVAKPGLDGHDRGAKVVARALSDAGYEV
                     IYTGLHQTPAMIAATAVQEAVDAVGLSILSGAHMTLFPAVLDALRSRGAGDVLVFGGG
                     IIPEEDETELRKQGVAALFKPGTSTRDIIDWVEKEVRPRAEQSRLK"
     gene            complement(1471..2646)
                     /locus_tag="PKL17_15775"
     CDS             complement(1471..2646)
                     /locus_tag="PKL17_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK46244.1"
                     /translation="MHFEPSEEQRLIETTARQFAEQVLKPAAAERDQKGLFPDKELRL
                     LGELGLLGVNVPSQYGGSEAGAVAYSLAVSEVAAADASVSVAMAVTNMVAEVICAFGS
                     EAQKTTYVPRLCSGEAIAGAFALSEPQAGSDAASLQTVAERVPGGYRLTGNKQWITSG
                     DRAGVIVVWARTDGGKADKRSQAGGLSAFIVEGGAQGLVAGRPEHKLGLKGSTTVPLL
                     LDGCFVPESARLGNEGDGFKIAMVALDGGRIGIASQAVGVGRAALSAAKNYAKERKQF
                     GQAIADFQAIGNMLADAATQLDAARLLAVRAAYLKEQKLPFSQKAAMAKLYASEAAGR
                     ICDMALQVHGGYGYTQEFPVERYLRDARVQRIYEGTSEIQRLVIARSVLKEFSDHAS"
     gene            complement(2671..3432)
                     /locus_tag="PKL17_15780"
     CDS             complement(2671..3432)
                     /locus_tag="PKL17_15780"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK46245.1"
                     /translation="MLRLGKWMPLQMALCGALLCGALLGTAQSKKKPKPAPPPPAAEE
                     MAIEGIAGQLDAAACLAVLETNKAQLKKCYEDVSSRLRFLSGHVELKMFIGTDGKAKR
                     IITQKSNLGAYEVEHCLLTTLGGISYPPAKGGLGEITYPMDFAAMVPSTPLGREQASE
                     QLDKEKKKLLSCAAKAKKPKGNEKFAAIEAVKMTGYVAPGGQVASVGFSSPKDPLDEA
                     VTACLDSKARSLKLPDPLGKIVKFEYSLGAADDGE"
     gene            complement(3442..3981)
                     /locus_tag="PKL17_15785"
     CDS             complement(3442..3981)
                     /locus_tag="PKL17_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46246.1"
                     /translation="MSSHLDPSCQTQPGRARAQSLRLGFGLGGVLLAALVIQAGCDEK
                     SPQTRSGGAQLRTSGKGGSTTQQVAPGGGYVEQSENAEDRAESVRRVCQRKASSDLPL
                     CWSAEVDRSKNRKLEARIGIMLSVSPQGKADKVDVISPQPELSHLEQCVVDAARSWSY
                     PDGTATALVRCDFYLRSSQ"
     gene            complement(4019..5170)
                     /locus_tag="PKL17_15790"
     CDS             complement(4019..5170)
                     /locus_tag="PKL17_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNK46247.1"
                     /translation="MDFRLSDEQLMAQKTAAEFAKSEVLPRAAEIDREHRFPAELVRR
                     MAELGFLGIAIPDQYGGAGLDTVSYVLALEEISRACASTGVIMSVNNSLVCDPLLKFG
                     TEEQKRKWLAPLASGQLLGCFALSEPEAGSDAAAQKTVAVKDGDGWVITGVKNWITNG
                     PNANVCILQCMADPPGKHVGHKAITTFILPMDMPGVRTGPADKKLGIRGSQSCQIFLD
                     EVRVGRDLLLGDVGVGFKVAMSTLDGGRIGIAAQALGIARASFEDAGRYALERKTFGQ
                     PIANHQAIQWKLADMATELDAARLLTLRAAQLKDKGQRHSKESAMAKLFASECANRAA
                     KECVQIFGGNGYVEDYPAERHYRDAKITEIYEGTSEIMRIVIAASLLKE"
     gene            complement(5297..6085)
                     /locus_tag="PKL17_15795"
     CDS             complement(5297..6085)
                     /locus_tag="PKL17_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNK46248.1"
                     /translation="MQSQYETLVVELDGPVCVVQINRPKALNALNQQVLTELLRLTSE
                     LATSETVRAVVLTGAGEKAFVAGADIAAMAQMMPPEALRFAELGHRLMDSLEALPQPV
                     IAAVNGFALGGGMELAMSCDILLASENARFGQPEVNIGVMPGFGGSQRLPRRVPFGVA
                     AEILLSGDNITAADALRIGLVNRVLPFAELITEAKKLAHKMACRAPVAIAKTKQALHA
                     SRQTGLADGNARERQLFSELFATADQKEGMGAFLQKRPPSYTGK"
     gene            complement(6096..6953)
                     /locus_tag="PKL17_15800"
     CDS             complement(6096..6953)
                     /locus_tag="PKL17_15800"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNK46249.1"
                     /translation="MSETAIQKLGIVGAGQMGAGIAQVAAERGVAVVLVDRDDETAQK
                     GKAKIAAQLGKLVEKGKLAAEKRDAAVALIATSGSYEALAECDFIVEAATENQELKQK
                     IFEKIDAAAKPGAVLATNTSSISITLLASKTKRPEQVIGMHFMNPVPLMTLCEIIRGL
                     QTSDATYQATLALGKALGKELVTARDVPGFIVNRILIPMLNEACFALAEGLGTAEDID
                     KGVKLGLNHPMGPLALADLIGLDTVLAIAEVLHRELGDPKYRPAPILRSHVAAGFLGR
                     KTGRGFYKY"
     gene            complement(6977..8173)
                     /locus_tag="PKL17_15805"
     CDS             complement(6977..8173)
                     /locus_tag="PKL17_15805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNK46250.1"
                     /translation="MTASAHGREVVIVSAARTPIGAFAGELAQVAAPELGAIAIKAAL
                     ARASVSPADVGEVTMGCVLQAGIGQAPARQAAIKAGIPTSVPCQTLNKVCGSGLKAVI
                     LAAQAIALGEVEVAVAGGMESMSNVPYYLLTARGGFRMGHQQATDGMIFDGLWDPYNN
                     FHMGSAAEVCARDKKIDRQSQDAYAAESYKRAQAAQAAGKFDAEIVPVEIAQKKGPAK
                     VVSRDEGPSRGSDAAKLAALRPAFEKDGTVTAGNASSINDGAAAVVLMSAEEAARRGL
                     TPLARIRSWAQHAQEPAWFTTAPAFAIEKALSRAGWQKNEVDYYEINEAFAVVALVNN
                     QLLGISSDNVNPFGGAIALGHPIGASGCRLLVTLVHSLQREGKRRGVVSLCIGGGEGI
                     ALTVER"
     gene            complement(8336..8656)
                     /locus_tag="PKL17_15810"
     CDS             complement(8336..8656)
                     /locus_tag="PKL17_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46251.1"
                     /translation="MPGSQHVPGRASSLPIAPVDQAVDGATDYASTEGEDSAFLLCRK
                     LRSKLAFAQVESSDGEHVSAIDALQGTAVYWCLSTMECAGPDGGLAHQSVCRSDRGCY
                     KSRV"
     gene            8808..9512
                     /locus_tag="PKL17_15815"
     CDS             8808..9512
                     /locus_tag="PKL17_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46252.1"
                     /translation="MTATIATTSGCDLMRMVRDTSQGIQETNKQLREASGLIRQSGQL
                     IGQASDSMTKMQPSLDSVAHLDGSMKALAEMKGSMDRLAAMQTDFDSMAKGMRDLAAM
                     RPAMEQMATLGPQLAAVARLGGSMGDVAGLKPTLQAVADLKQPMDNLTKLADPMNRVS
                     RLEGPLEKTAALVTPMNTLAAQVDSLERAQSVHIVWWSIFGLSLWMLATAMGAAAGVY
                     IGNRMSSSKKNRTAEA"
     gene            complement(9548..10795)
                     /locus_tag="PKL17_15820"
     CDS             complement(9548..10795)
                     /locus_tag="PKL17_15820"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNK46253.1"
                     /translation="MIRGQRTLSLVTCSLLGAWSTAALASPASAKRPCGMSDLTVEVY
                     ALGGVIRPSLADSMFNSPARTGTSGTAEQDFTARGSDLGFNQPWMPTIEGGAIVFPCR
                     FFGIGVHGGGSWSGLPDGQPTSPQLAQRTTQQLSMMRMSLSLHGQLPISRYFVLRASA
                     LIGYRNYALGVAGNGQREALLEQVSLEPRLEARLRLPLAVPFGVAAFVGGEVLSQPAL
                     TVGGGLFIATDWPDPIRRGAAEPTPDKATTAGGTAGAAPENPPPVITPPVITPPPVEA
                     PQVVTPVTPIQPVTPVTPIQPVTPPPIETPMVPVPVTPPPVETPVVPAPVTPPPSPTP
                     TVTPPVVVNGLAFMDQNATGLVNTQSDVLNVRSGPAKTAPILGTVAKGARVNITGVAP
                     GWYRIRFKDGIGYVSGDFILREP"
     gene            10914..11083
                     /gene="ssrS"
                     /locus_tag="PKL17_15825"
     ncRNA           10914..11083
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKL17_15825"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11193..>11642
                     /locus_tag="PKL17_15830"
     CDS             11193..>11642
                     /locus_tag="PKL17_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46254.1"
                     /translation="MPLFDGFKKDEEPKLSPWLPQLGHAPSQETGPKPEEKHDLPPLA
                     AVQQNPSGKQSETVKENVKEQKKEADKKSDSGGHDEPYVPFEDMFHEKPDFEKYKKQD
                     KLPDKYVIKDETDAKQMQYSSFGVPMTPDAWAMQTAIDGGKLHYLYDK"
BASE COUNT         2215 a         3765 c         3400 g         2262 t
ORIGIN      
        1 gcaagcccgc atcgccgctt gcgccgcata cagcttcgcc atcgacgctt gcaggccaaa
       61 cttcatccct tgatcgtgca tccacgccgc ccgctgaaga agcagccgcg ccgcatcgag
      121 ttccatcgcc gcctgcgcaa tcatgttttg gatcgcctgg aactcggcca gcgacttgcc
      181 aaactggcga cgctctttgg cgtagcgaat cgccgccgca gtcgcccctg cccccagccc
      241 gagcgccatc gccccgatgg tgattcgccc tttgtccaag atccgcagtg tgtcgatgaa
      301 gccgtggtcc agttcgccaa gccgctggct gtccggcacc cgcacgtctt cgagcagaag
      361 ctccgcggtg tccgacgcat gcagacccat tttttcgatc cgccggctcg ccgaaaaacc
      421 cggtgtgccg tagtcgacaa tgaacgccgt gatgccgcgc tgtttctttt ccggtgacgt
      481 gaccgccaaa atcaccgcca caccaccgac ggaaccttgg gtgatgaagg ttttctgccc
      541 gctgatgctc cagcttccgt cggcaagccg cgtcgcccgc gtcttggccc ccgccgcgtc
      601 cgatcccgaa cccggttcgg tcaatcccca tgcgcccagc tttgtccccg acgccaggtc
      661 cggcagatag cgcgactttt ggtcttcgtt gccaaacagg ctgatatgac ccgagcacag
      721 accgttgtgc gacgcgaccg taatcgccac cgagccatcg accgcagcca gctcctcgat
      781 gacgagcgca acttcctgca tcgtcatcgc cgacccgcca tacgcttcgg gaatcgtcat
      841 tcccaaaagc ccgagcgcgc cgagcttggg cacaagctcg atgggaaagt gcgactcgtt
      901 atcccacttg cgactgtgcg gtgcaatttc ggcctgcgca aactcacgca cggtcttttg
      961 cagcgctttt tggtcttcgc ttagctcaaa gtcaatcatg gtgttctcgt cgctgtgttt
     1021 ggaaagcgag cagcttactt taggcggctt tgctcggcgc gcggacggac ttctttttcc
     1081 acccagtcga tgatgtcgcg cgtcgatgta ccgggcttaa acagcgccgc gacgccttgc
     1141 ttgcgcagct cggtttcgtc ttcttcggga atgatgccac cgccgaacac cagcacgtca
     1201 ccggcaccgc ggctgcgcag cgcatcgagc accgccggaa acagcgtcat gtgcgcaccc
     1261 gacaaaatcg acagccccac ggcgtcgacc gcttcttgca ctgccgtcgc tgcaatcatc
     1321 gcgggggtct ggtgcaggcc ggtgtaaatc acttcgtatc ctgcgtcgga aagcgcacgc
     1381 gccaccactt ttgcgccgcg gtcatgtcca tcgaggccag gcttggcgac gaggatgcgg
     1441 agcttctgct tggcactgcc aaaagacgtg tcagcttgca tgatcggaaa actccttcag
     1501 caccgaacga gcgatcacca gccgctgaat ctcgctggtt ccctcgtaaa ttcgctggac
     1561 gcgggcgtcg cgcaggtagc gctcgaccgg gaattcttgg gtgtagccgt agccgccgtg
     1621 gacttgcagc gccatgtcgc agattcgacc ggctgcttcc gatgcgtaca gcttggccat
     1681 cgccgctttt tgcgaaaacg gcagcttttg ttctttcaga tacgccgcgc gcacagccaa
     1741 aagacgcgcc gcatcaagct gggtcgcagc gtcggcgagc atgttgccaa tcgcctgaaa
     1801 atcggcaatc gcctggccaa actgtttgcg ctctttggca tagtttttcg ccgcggatag
     1861 tgctgcccgt ccgacgccga ccgcttggct ggcgatgcca atccgtccgc cgtcgagagc
     1921 gaccatcgca attttgaagc cgtcgccttc gttgccaagc cgcgccgatt cgggcacaaa
     1981 gcagccgtcc aaaagcagcg gcaccgtcgt cgaacctttc agtccgagct tgtgttctgg
     2041 ccgtccggcg acaagccctt gtgcgccgcc ttcgacgatg aacgccgaca gaccaccggc
     2101 ttggctgcgc ttgtctgctt tgccaccgtc tgtgcgcgcc cacaccacga tgacgcccgc
     2161 gcgatcgccc gacgtaatcc actgcttgtt gcccgtcagc cgatagccgc ccggaacccg
     2221 ctcggccacc gtctgaagcg acgcggcatc cgatccggct tgcggttccg acagcgcaaa
     2281 cgcccccgcg atcgcttccc ccgagcacag ccgcggcaca taggttgttt tctgcgcttc
     2341 acttccgaac gcgcagatca cctcggcgac catgttggtg acggccatcg caacggacac
     2401 cgacgcatcg gcagcggcca cttcgctgac cgccagcgaa tacgccaccg ccccggcttc
     2461 agacccgccg tactgcgacg gaacattgac gcccaaaaga cccagttccc cgagcagtcg
     2521 cagctctttg tcgggaaaca gtcccttttg gtcgcgctcc gccgctgccg gcttcagcac
     2581 ttgctcagca aactgtcgcg ccgtcgtctc gataagtcgc tgttcttcgg aaggctcaaa
     2641 gtgcatggac tgctcgattc cctttgtgcg ctactcgccg tcgtccgcgg cgcccagcga
     2701 atactcaaac ttcacaatct tgccgagcgg atcgggcagc ttcaggctgc gcgccttgct
     2761 gtcgagacac gccgtcactg cttcgtcgag cggatctttg ggcgacgaaa aaccgaccga
     2821 cgccacctgt ccaccgggcg ccacatagcc ggtcatcttc accgcttcga ttgcagcaaa
     2881 cttttcattg cccttgggtt ttttggcttt ggcagcgcag ctcagcagct ttttcttttc
     2941 cttgtcgagc tgctcggacg cttgctcgcg acccagcggc gtcgacggaa ccattgcggc
     3001 aaagtccatc ggataggtga tctcgccgag tccgcctttg gccggcggat agcttatgcc
     3061 gccgagtgtg gtcagcaagc agtgttcgac ttcatatgcc ccaaggttgg atttttgcgt
     3121 gatgatccgc ttggctttcc cgtcggtgcc aatgaacatc ttgagctcga cgtgaccgct
     3181 tagaaaacgc agccgcgacg acacatcctc gtaacacttt ttgagctggg ctttgtttgt
     3241 ctccagcacc gcaagacacg ccgcagcatc aagctgtccg gcaatgcctt ctatcgccat
     3301 ctcttccgcc gcgggcggag gcggggccgg ctttggcttc tttttcgatt gcgccgtccc
     3361 aagcagggct ccgcaaagca gcgcgccaca aagcgccatt tgcaaaggca tccactttcc
     3421 cagcctcaac atcggttctc cttactggct gctgcgcaga taaaaatcgc agcgcaccag
     3481 cgccgtagcc gttccatcgg gataactcca gctccgcgcc gcgtcgacca cgcactgttc
     3541 gaggtgcgac aattcgggct ggggcgaaat cacatcgact ttgtccgctt tgccttgcgg
     3601 cgaaacgctc agcatgatgc cgatgcgcgc ttcgagcttg cggttcttgc tgcgatccac
     3661 ctcggcgctc cagcacagcg gcagatcact tgaggctttg cgctgacaga cccgtcgcac
     3721 cgattcggcg cggtcttcgg cattttcgct ctgctcgacg tagccgccac cgggagccac
     3781 ttgctgcgtc gtcgaaccac ccttcccact cgtccgcagc tgcgcaccac ccgaccgtgt
     3841 ctgcggcgat ttttcgtcgc agccagcttg gatgacaagc gcagcaagca ggacacctcc
     3901 gagtccaaaa ccaagccgaa gggactgcgc gcgggcgcga ccaggctgcg tttgacagga
     3961 tggatcgagg tgagaagaca tggcggactc ctttacaaag acttgccgac ggaccgacct
     4021 attccttgag cagcgaggcg gcaatcacaa tccgcatgat ctcgctggtg ccttcgtaaa
     4081 tctcggtgat cttggcgtcg cgatagtgcc gctcggcggg gtagtcttcg acgtagccgt
     4141 tgccaccgaa aatctgcacg cactctttgg cagcgcggtt ggcgcattcc gacgcaaaca
     4201 gcttcgccat cgccgactct ttgctgtgcc gctggccttt gtcttttagc tgcgccgccc
     4261 gcaaagtcag aagccgcgcc gcatccagtt ccgtcgccat atcggcgagc ttccactgaa
     4321 tcgcttggtg gtttgcgatc ggctggccaa acgtcttgcg ttccagcgcg tagcgaccgg
     4381 catcctcgaa cgacgcacgg gcaatgccca gcgcctgcgc cgcgatgccg atgcgcccac
     4441 cgtcgagcgt cgacatcgcc actttgaagc cgacgcccac atcgcccagc aaaagatccc
     4501 gaccgacgcg cacttcatcg aggaaaatct ggcacgactg cgaaccgcga atgcccagct
     4561 ttttgtcggc tggaccggtt cgcacgcccg gcatgtccat cggcaaaata aacgtcgtaa
     4621 tcgctttgtg tccaacgtgc ttgcccggcg ggtcggccat gcactgcagg atgcagacgt
     4681 tggcgtttgg accgtttgtg atccagtttt tgacgcccgt gatgacccag ccatcgccat
     4741 ctttgacggc gacggtcttt tgcgccgcag cgtctgaacc ggcttccggc tccgacagcg
     4801 caaaacagcc aagcagctga cccgatgcga gcggtgccag ccatttgcgc ttctgctctt
     4861 cggtcccaaa cttgagcagc gggtcacaga cgagcgagtt gttgacgctc atgatgacgc
     4921 cggtcgacgc gcaggcccgc gagatttctt cgagcgccag cacatacgac acggtatcga
     4981 gcccggcgcc gccgtactga tcgggaatgg caatgcccaa aaagcccagc tcggccatgc
     5041 gacggaccag ctcggcggga aaacggtgtt cgcgatcaat ctcggcagcc cgcggcagca
     5101 cttcgctctt ggcgaactcg gcagccgtct tttgggccat taactgctca tcgctgagac
     5161 ggaaatccat ggcagtcgct tttccttagc aaatgggagt ggggcagtct gaaaaagacc
     5221 gccgcgccat gcttatcacg ttctcacgag tgcgggtagc aatcaaaccg ccgacccgcc
     5281 acgagagaaa gtcggtttat ttgccggtgt agctgggggg acgtttttgc aaaaaggcgc
     5341 ccatgccttc tttttggtct gccgtcgcga acagctctga aaaaagctgg cgttcccgcg
     5401 catttccgtc ggcaagcccc gtctgtcgcg acgcatgaag cgcttgcttg gtcttggcga
     5461 tggccaccgg ggcgcgacag gccattttgt gcgccagttt tttggcttcg gtgatcagct
     5521 cggcgaacgg caacacacgg ttgaccagcc cgatccgcag cgcatctgcg gcggtgatgt
     5581 tgtcgcccga aagcaggatc tcggcagcga ccccaaacgg cacccgtcgc ggcaagcgct
     5641 ggctgccacc aaaaccgggc atcacgccga tgttgacttc tggctgacca aagcgggcat
     5701 tttccgacgc cagcagaatg tcacacgaca tcgccagttc catgccaccg ccgagcgcaa
     5761 aaccgtttac ggctgcaatc accggctgcg gcagcgcttc gaggctgtcc atcaaccgat
     5821 gacccagctc cgcaaaacgc agcgcttcgg gcggcatcat ctgcgccatc gctgcaatgt
     5881 ccgcgcccgc gacaaaggct ttttcccccg cgccggtcag gacgaccgca cgcacggttt
     5941 ccgatgtcgc cagctcgctg gtcagacgca gaagctcggt gagcacctgc tggtttagcg
     6001 cattgagcgc tttgggacga ttgatttgga cgacacagac ggggccgtcc agctcgacaa
     6061 cgagcgtctc gtattggctc tgcatggccg ttctcttagt acttgtagaa accgcggccc
     6121 gttttgcgtc ctaaaaagcc cgcggcgacg tggctgcgca aaatcggcgc cggacggtat
     6181 ttgggatcac ccagctcgcg gtgcagcact tcggcaatcg cgagcacggt gtcgagccca
     6241 atcaggtcgg ccagcgccaa tggccccatc gggtggttta ggccaagctt tacgcctttg
     6301 tcgatgtcct cggcggtgcc cagcccctcc gcgagcgcaa agcacgcttc gtttagcatc
     6361 ggaatcagga tgcggttgac gataaagccc ggcacatcgc gcgccgtcac cagttctttg
     6421 cccagcgctt tgccgagcgc cagcgtcgct tgatacgtcg cgtcgctggt ttgcaggccc
     6481 cggatgattt cgcacagcgt catcagcggc accggattca taaagtgcat gccgatgacc
     6541 tgctcgggcc gcttggtctt gctcgcaagc agcgtgatcg aaatcgatga cgtattggtc
     6601 gccagcaccg cgcccggctt ggccgccgcg tcgatttttt cgaaaatctt ctgcttcagc
     6661 tcttggtttt ccgtcgctgc ttcgacgatg aaatcacact cggccagtgc ttcatagctg
     6721 cccgaggtcg caatcagcgc cactgccgca tcgcgctttt ccgccgccag cttgcccttt
     6781 tcgaccagct tgccaagctg cgccgcgatc ttggctttgc ctttttgcgc cgtttcatcg
     6841 tcgcgatcga ccagcacaac cgccacgccg cgctcggcag cgacctgggc aattcctgcg
     6901 cccatctggc cagccccgac gattcctagc ttctgaatag ccgtctcact catcgatacc
     6961 tcacccgaaa tgttccttaa cgctcgacgg tgagcgcaat tccttcgccg ccgccgatgc
     7021 acagcgaaac gacgccgcgc cgcttgcctt cgcgctgcaa gctgtgcaca agcgtcacca
     7081 aaagacgaca gcccgacgca ccgatcggat gacccagcgc aattgcgcca ccgaatggat
     7141 tgacgttgtc gctcgaaatg ccgagaagct ggttgttgac gagcgccacg acggcaaacg
     7201 cttcgttgat ttcgtaataa tcgacttcgt ttttctgcca accagcgcgc gacagggctt
     7261 tttcgatcgc gaacgccggt gccgtcgtaa accacgccgg ctcttgcgcg tgctgcgccc
     7321 atgaacgaat ccgcgcaagc ggcgtaagac cgcgacgagc cgcctcttcc gcagacataa
     7381 ggacgactgc cgcggcgccg tcgttgatgc tcgatgcatt gcccgccgtc accgtgccgt
     7441 ctttttcaaa cgctgggcgc agcgccgcaa gcttggccgc atccgatcca cgcgacggtc
     7501 cttcgtcacg cgacaccact ttggccgggc cttttttctg ggcgatttcg acgggaacaa
     7561 tctcggcatc gaacttgccc gctgcctgcg ccgcctgcgc ccgcttgtac gactccgccg
     7621 cgtaggcgtc ttggctttgg cgatcgatct ttttgtcacg cgcacagact tccgcagccg
     7681 atcccatgtg gaagttgttg taggggtccc acagtccgtc gaagatcatg ccatcggtcg
     7741 cttgctgatg acccatgcgg aagccgccgc gcgccgtcaa aaggtagtac ggcacattgc
     7801 tcatcgattc catgccaccc gcgacggcaa cttcgacttc cccgagcgca atcgcctgcg
     7861 ccgccaagat caccgcctta agccccgacc cacagacctt gttcagcgtc tgacacggca
     7921 cagacgtggg aatcccggct ttgatcgcgg cctgacgggc cggcgcttga ccgataccag
     7981 cttgcagaac acagcccatc gtcacttcgc ccacgtccgc gggactgacc gaagcgcggg
     8041 ccagcgccgc tttgatcgcg atggcgccaa gctcgggagc cgccacttgc gcaagctcac
     8101 cagcgaacgc gccaatcggg gtacgagcgg cagagacgat tacaacctca cggccatgtg
     8161 ccgaagctgt catgggaaga ctccttgtcg atgtcgtttc tgcgcaagca agtgacaggg
     8221 atgctgattt cactcgctgc ggcaaggcga ccttgccaca aagatccact gccgagcaag
     8281 cggcgctacg cgggccgata ctgtgccgag cgacgtcacc gcgtcgaaag cgggttcaga
     8341 cgcgcgactt gtaacagccg cgatcggaac ggcagacgct ctggtgagcc aagccaccgt
     8401 cgggtccggc gcattccatc gtcgacagac accaatacac cgcggtgcct tgcagcgcat
     8461 cgatcgccga gacgtgttcc ccgtcgctcg attccacttg agcaaacgcg agcttgctcc
     8521 gcagtttgcg acacagcaaa aacgcgctgt cttcgccctc tgtcgatgca tagtcggtgg
     8581 caccgtcgac ggcttggtct acaggagcaa tcggaaggga agacgcgcga cctgggacgt
     8641 gctggctacc gggcatggct ttcgcacgct agcagagaaa atgcccagcg tgcaaaagga
     8701 cttaccgtcc gctgcctctc gctgtcatgg acgtgtgtta ccgtccccgg tgaggttttt
     8761 tggcgatggc tacacgacgg ctcatgggaa gaggcgctgc gcttttcttg accgcgacga
     8821 tcgcgacgac atcgggctgt gacctgatgc ggatggtgcg ggatacgtcg cagggcattc
     8881 aggaaaccaa caagcagctg cgtgaggcga gcggtctgat tcggcagtcg gggcagctta
     8941 tcgggcaggc gtcggattcg atgacaaaaa tgcagcccag cctcgatagc gtggcgcacc
     9001 tggacgggtc gatgaaagcg ctggccgaaa tgaaaggatc gatggatcgg ctggcggcga
     9061 tgcagaccga cttcgattcg atggccaagg gcatgcgcga tctggcggcg atgcgtccgg
     9121 cgatggaaca gatggcgacg ctcggtccgc agcttgcagc agtggcgcgg ctcggtggat
     9181 cgatgggaga tgtcgcggga ctgaagccga cgctgcaagc ggtcgcggat ctcaaacagc
     9241 cgatggacaa cctgaccaag ctggcggacc cgatgaaccg ggtgtcacgg ctcgaagggc
     9301 cgctcgaaaa aaccgcggcg ctggtcacgc cgatgaacac gctggcggcg caggtcgatt
     9361 cgctggaacg cgcccagtcg gtccacatcg tgtggtggag catctttggt ctgtcgctgt
     9421 ggatgctggc gacggcaatg ggcgcggcgg caggcgtcta catcggcaat cggatgtcga
     9481 gcagcaaaaa gaaccgcacc gccgaggcat agtttcgacc cgcggcggtg tgcgatggaa
     9541 attttgctta cggctcgcgc aggatgaagt cgccgctgac gtagccgatg ccgtctttga
     9601 agcggatgcg ataccagcca ggagcgacgc cggtgatgtt tacgcgcgcg cccttcgcca
     9661 cggtgccgag gatcggcgcg gtcttggccg gaccgctgcg cacgttgagt acgtcgcttt
     9721 gggtgttcac aagtccggtg gcgttctgat ccataaacgc caggccatta accaccacgg
     9781 gcggcgtcac cgtcggcgtg ggagagggtg gcggcgtcac cggtgcgggc accacaggcg
     9841 tttcgacggg cggcggcgtc accggcacgg gcaccatcgg cgtttcgatc ggcggcggcg
     9901 tcaccggctg aatcggcgtc accggtgtca ctggctgaat cggcgtcacc ggtgtcacca
     9961 cctgaggcgc ttcgacaggc ggcggcgtaa tcacaggcgg cgtaatcaca ggcggcggat
    10021 tttctggtgc agcgcctgca gttccaccag ccgtcgtcgc tttgtcaggc gtgggctcgg
    10081 cagcaccgcg cctgattgga tcgggccaat cggtcgcaat gaacagacca ccaccgacgg
    10141 taagagccgg ctgcgacagc acctcgccac cgacgaaagc cgcaacacca aacggcacag
    10201 ccaacggcag ccgcaggcgc gcttcgagtc gaggctccag cgacacttgt tccagcaggg
    10261 cttcgcgctg gccattgccg gcgaccccga gcgcatagtt gcggtagccg atgagcgcgc
    10321 tcgctcgcag cacaaagtaa cgagagatcg gaagctggcc gtgcaacgac agcgacatcc
    10381 gcatcatcga aagctgctga gtggtccgct gggcaagctg cgggctcgtt ggctgtccgt
    10441 cgggaaggcc gctccacgaa ccgccgccgt gaacgccgat gccgaaaaag cgacacggga
    10501 agacgatcgc tccgccttcg atggtcggca tccacggctg gttaaagccc aggtcactgc
    10561 cgcgggcggt gaagtcctgt tcggcggtcc ccgacgtgcc tgtccgcgct ggcgagttaa
    10621 acatcgaatc ggccaagctg ggccgaatca cccccccgag cgcgtaaacc tcgacggtaa
    10681 gatcggacat gccgcaggga cgcttggccg aagctggact ggccagtgca gccgttgacc
    10741 acgcgccaag cagcgagcaa gtcacaagag acagtgtgcg ttgaccccga atcacgatcg
    10801 ccccctagta agttctgata gccctttgag attttctcac atcacatttg cttctaccag
    10861 accttgacga cgacggcggt ggcacctagt gtaagactcg acctggctgc ctgaatcgcc
    10921 tcggttggga tagttttttc cctacaatgt tgaacctggg attcacctct ggccgtagcg
    10981 tgcacgcccg catgtcggga agcccatagc ggtcaatttt gtggagccca cctacttagg
    11041 taggggttca aacatcctga ccacgatcgg gcgggggtta gccgaccctt cggggtcggt
    11101 ttttttttcg tctgcacgcc aggttctggc atgcggaagc gcgagcggaa actcgatctg
    11161 ggtgggggtc gataactcga tcggaggatc ggatgccgct cttcgatggg ttcaaaaaag
    11221 acgaagaacc gaagctttcg ccatggctgc cccagcttgg acatgcgccg agtcaagaaa
    11281 ctgggccaaa gccagaagaa aagcatgact tgccgccgct cgccgccgtg cagcaaaacc
    11341 ccagcggcaa gcagagcgag acggtcaaag aaaacgtcaa agagcagaag aaggaagccg
    11401 acaaaaagtc ggactccggc ggccacgacg aaccctacgt cccatttgag gacatgtttc
    11461 acgaaaagcc ggacttcgag aagtacaaga agcaggacaa gctgccggac aagtacgtca
    11521 tcaaagacga aaccgacgcc aagcagatgc agtacagctc gttcggcgtt ccgatgacgc
    11581 cggatgcgtg ggccatgcag accgcgatcg acggcggcaa gcttcactat ttgtacgaca
    11641 ag
//
LOCUS       DAORGN010000160        11633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2310_length_11633_cov_5.836846, whole genome shotgun sequence.
ACCESSION   DAORGN010000160 DAORGN010000000
VERSION     DAORGN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_11633_cov_5.836846"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKL17_15835"
     CDS             complement(<1..1441)
                     /locus_tag="PKL17_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46255.1"
                     /translation="MFDDEADDVGQEIEAFCARCKTDTPHTVVSRYDEEIRRVRCNTC
                     DDVHAFRRPRGEDGDEPSEQPSRKKLIKAKPTWEQVINKKKSTPRAWGPDEVFCELDV
                     LTHSVFGVGFVSELIGQNKIEVTFQNDKRILIHNRRLLPLHLVQRQRQEAEARARADG
                     KAKAKEAREAKAAAREAARLAKAHEPELDDLDLPDMLHDDEEIPVVSSPKSGGMGKTT
                     KLSARELAALDDDDDDDSLIGDLDDEGDAPRGRGRKAKGAEPPVGRGRPAASKFADAG
                     DDDDDDADDDFDEDRPSSKSKSSPDGKRRVGRPPGKKNAKADAEEKPAPSSKSSKTAA
                     PKGTDSDDAPKAKKADKSKAEPASGKAAKADKSAGKALAGVAKADSAAKSESADKPGK
                     GKTDGGEGKAGGKSAPVAAKDAASGDKAKKDAKSAKSAPTSEPAKPAAKATEKPAKAE
                     PVKKADKAAAKAEPAKKADKASAKSEPAKA"
     gene            1763..2851
                     /locus_tag="PKL17_15840"
     CDS             1763..2851
                     /locus_tag="PKL17_15840"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNK46256.1"
                     /translation="MLGKMLAAAGIALAALQEGCYAAHYLGQQAGGQLHLLRIRRNVS
                     EVISDPTTEEALRQRLQLVMEARKFGIETLGLRGGAEFTRYVDPGGPVAYNLTVSPKD
                     ALTVRSFRFPLVGRVPYLGFFDLRDAKREAQRFADAGFDVYLRPVGAYSTLGYLISPI
                     YRSMLGDFSAAGELRAVETILHEMAHSTVFLPGASDLNESFATQVGIRGAALFYRSRG
                     DLTQSDRVFALAKVGEADEKSFSTWLAPQIDRLNRFYKRAAEVKLRRKQLLEQREAIF
                     EEIRQSYRQAFPEGRRYKRLADGPLSNALLSSFAVYHESSGLQEALLAICDDDLKRFV
                     SLYREARDRRDGAEWLRRLADLQRRSVP"
     gene            complement(2828..4381)
                     /locus_tag="PKL17_15845"
     CDS             complement(2828..4381)
                     /locus_tag="PKL17_15845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNK46257.1"
                     /translation="MTKTRLSAWIGSLLLGTAALANASPAQPLSDAPALSLDELQLAA
                     AIRSQQALPDLVVDLDDRVDDLTVRVLARRYSVRFVADSSYVGHDRIYRVFIDKTGDQ
                     ASRLSSLLRALRHDPLVEAADLELLYQIPETAPGALTSDDRAGRIPASSESPKPADRD
                     GKPRFQPSDPKYRYQWHLDQINMPEAWMLSRGQGVTVAVIDTGVTKVEDLHGTELVPG
                     WNFVNDNPDAQDDHGHGTHVAGTIAQTTDNGIGVAGVAPHARIMPIKVLSGSGSGSVA
                     GIAEGIRWAADHGAQVINMSLGGPFNSPVLANAVRYAHDHGVVVVCAAGNDGRGRVSY
                     PAANRGAIAVAATQFDETTTFYSNWGKEITIAAPGGNTRIDQNGDGVPDGVLQNTIVP
                     GDISRQDYLGFMGTSMASPHVAGVAALVVSQGVRDPDAVAKILKQTARRPVHYRGSHD
                     NHYGAGIVDALAAVQEARAQVAMQSQTAQDVPPVGIRLLALGLVAASLAYAGLRRRSF
                     LSSGNGTAL"
     gene            complement(4618..5355)
                     /locus_tag="PKL17_15850"
     CDS             complement(4618..5355)
                     /locus_tag="PKL17_15850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK46258.1"
                     /translation="MSSPPSSPSSFVFTPSHDEEVSATKALWRIARALPVVERTYAII
                     RFMILRVKLLATMDLLLPSDGPILDVGCGFGLWSAYFSQMQPGRRIIGIDPNAHRIDM
                     AKKVAERLGVKTEFYAEDVRQTAARGPFRAVYLLDVLHHIPSADQVDVVRQLTDMLEE
                     GGTLVIKDITTKPWFGLKFTEILDRAMVGMKEELTYRHHNEWSDLLRDLGYQVRVVRV
                     PDVLPYPHVIIAATKGSKPATTSAVLN"
     gene            complement(5401..7194)
                     /locus_tag="PKL17_15855"
     CDS             complement(5401..7194)
                     /locus_tag="PKL17_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46259.1"
                     /translation="MLLRLRAMSPRFRFLLASSLALTVGFTMSYCGNSVLGGDDPNAD
                     MGTEQPKTLAYLTVSPDNDVMQVDVNTTAQKTFTVFAHYSDGSAEDVSASSTLSLENT
                     AVGSLAGLTFTSAQSATAKVGFTKVNATYTTSSGQTVTGYANLTVVWLRLTGAATDFF
                     FKLPYMGGSQDQPLQFGTNVQSLDSFFAVDTTGSMGPEIQALKNSLTNTIIPGVKAAA
                     AKDAQFGVAAVEDFPVSPYGQANYFPPNLDDQPLILLQTITADIAAATKAVGLLLNGT
                     RPRGYGVDLPEGQIEALYQLATGVGNVINGVVNIPANTTGIGGAGFRKGALPVITMIS
                     DAVFHAKGENTTCTTSGGTDQIGYSAVASVNNAAHTRAETVTALNKICAKVIGVSAIV
                     TTVPTNCLATADLVQMANSTGAVVPPSAWDIPSRPVGCAAGQCCTGQNGAGEATDANG
                     ECPLVFKIPSAGTGLDAQVVSGISNVARFSKFNVTTQTSGNPTGDMGETLPAGKTTAD
                     FITAITPKDASAPPAPPTLPAPVISGNAFTKVYPGSTVRFSVTAQNTMVQPSTGPQIF
                     RATIKVLAGGCADLDQREVIILVPPKAPVVG"
     gene            complement(7423..7638)
                     /locus_tag="PKL17_15860"
     CDS             complement(7423..7638)
                     /locus_tag="PKL17_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46260.1"
                     /translation="MVRASPEDNPKVRYTNPAGQTDKESIMPERPIESEIPPETPPQS
                     EDGNNREGPLPLSDEQGDSGGELQQRA"
     gene            7805..8410
                     /locus_tag="PKL17_15865"
     CDS             7805..8410
                     /locus_tag="PKL17_15865"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK46261.1"
                     /translation="MIKWLVRLGSAVGLLLIVFVGVMLLLGGGGRALRTEARIDIARP
                     AVVVFPWLVEGPKLHKWVSGLESFEPQNSEPQVGQKVQMVLVRDGHRYEVTREFTAYT
                     PPTLLQVQLEHSEFSDQIEYTLVEHDGATTLTERSTAHYKDLLYRLLVPLLSRDTERS
                     MNESLATLKALVETDPMPIPPHPLPGQKGFHGCCAPEPEHK"
     gene            8441..11209
                     /locus_tag="PKL17_15870"
     CDS             8441..11209
                     /locus_tag="PKL17_15870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNK46262.1"
                     /translation="MTLRQILDTPPPMLLLEHPYAEFISLVDKPARYLGGEFQSVRKD
                     WSSAEVRIALAFPDVYEIGMSHLGTKILYSLLSKDPRILCERAFTPWVDMERELRKRK
                     LPLVSLESARPLKDFDVVGMSLQYELTYTNCLTLLELGGIPLRAEDRGEDDPLVLAGG
                     PVATHPEPIAPFFDCILIGEAEKVLPDVLLDWARWKKEGIDRRERLIRLSQRGYLYVP
                     SLYSVKMDADIHRQVVDQPIDPRVPGKVNRVYVPNLNEFPFPSDTPVPYAEAIFDRAS
                     IEIARGCTEGCRFCQAGMIYRPVRERDPESIVESVVQSVKRGGYDETSLTSLSTADFS
                     CILPLMKKVMGRLREERVSLSVSSLRAYGLSPEILDEMSSVRATGLTFAPEAGTQRMR
                     DVINKNITEEDIARSAENVFSRGWSRMKLYFMIGLPTETDEDVAGIIHTAVRLRQIGK
                     RIRRDAEVTVSVSNHVPKPHTPFQFCAQDSLNDLQRKQILLRELARRERIEFKYHDKQ
                     MSWLEGLFSRGDRRLADVILAAHRLGARFDSWDEQFDIGLWHKALAEVGIDPTPYFMT
                     MPILARLPWDHLDIGLEEDFLRSEYRKAMKDRLSPPCGKPYGSLLHHTNVEDALADER
                     RLVCFDCGIACDLTQMRQDRLVALRKLGAETRTVAPRSNEEVAKEQAERGQPRKKAAF
                     SQSEGQRYRLRYRKLGRAAFIGHLDAMRLLQRMLRRAGCEPIYTRGFHPKPDMVFGPA
                     LGLGVVSLGEVVDLRLERLSETGEYIQADELQQRLQQAAPEGFVIVEVQQLGAEDKAV
                     SRLITAADFAIGVPKDAAHTDGAQLSQVESWAQRPLVVERPGKDGGESKRVDVQQFLL
                     SAEHLGDPTSASVRDELGWPADYDVVRARVRIDNQGGVRPVELAQALLGAVPAGVRYA
                     RLGLVLSQ"
     gene            11271..>11633
                     /locus_tag="PKL17_15875"
     CDS             11271..>11633
                     /locus_tag="PKL17_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46263.1"
                     /translation="MSSPPSGETPTPPIADEATAPTLDPARAPTVHRSDRGTQSAELL
                     LVAPPGAIPDPLKQWERYDLVRPLGQGGMGTVFLAKDRKLGRAVALKFLRVPSIEAAE
                     RLLQEARAQARLDHNNICK"
BASE COUNT         2242 a         3460 c         3529 g         2402 t
ORIGIN      
        1 cggccttggc tggttcagac ttggccgatg ccttgtcggc tttcttggcc ggctccgctt
       61 tcgccgcggc cttgtcggct ttcttcacgg gttcggcctt ggcaggcttt tccgtcgctt
      121 tggcagcggg cttggccggt tcagaagtag gtgcggactt ggccgacttg gcgtcttttt
      181 tggccttgtc accgctcgcc gcatctttgg ccgctacagg agccgacttg ccgcccgctt
      241 tgccctcacc gccatcggtc ttgcccttgc ccggcttgtc tgccgattcg gacttggctg
      301 cgctgtctgc tttcgccacg ccggccaagg ccttgcccgc tgacttgtcg gccttggccg
      361 ctttgccgct cgcgggttcg gctttcgatt tgtcggcttt tttggccttc ggcgcgtcgt
      421 cagaatcagt ccccttcgga gcggcagtct tgctcgactt gctgcttggg gctggttttt
      481 cttcagcatc ggctttggcg ttctttttgc caggaggccg accgactcga cgctttccat
      541 ctgggctcga cttcgacttg ctcgacggac ggtcttcgtc aaagtcatcg tccgcgtcgt
      601 catcatcgtc gtcgccagca tcggcaaact tgctcgcggc aggacgaccc cgacccacgg
      661 gtggctctgc gcctttggct ttgcgaccac gaccgcgcgg ggcatcgccc tcatcatcca
      721 ggtcgccgat gagcgagtca tcatcgtcat catcgtcgag cgccgccagt tcacgcgccg
      781 acagcttggt ggttttgccc atgccacccg acttcggcga cgaaacgacc ggaatctctt
      841 cgtcgtcgtg cagcatgtcg ggcaagtcca gatcgtcgag ctcgggctca tgcgctttgg
      901 ccaaacgcgc tgcttcacgc gctgcggcct tggcttcacg cgcttccttg gctttggctt
      961 tgccgtcggc tcgcgctcgc gcttctgctt cctgacgctg gcgctgcacc aaatgcaggg
     1021 gcaaaagccg gcggttgtgg atcaggatgc gcttgtcgtt ctggaacgtg acctcgatct
     1081 tgttctggcc aatcagctca gaaacaaaac cgacgccgaa caccgaatgg gtcagaacgt
     1141 ccagctcgca gaagacttcg tcggggcccc aagcccgcgg cgtgctcttc ttcttgttaa
     1201 tgacctgttc ccaggtcggt ttggctttga tcagcttctt ccgactgggc tgttcactcg
     1261 gctcgtcgcc gtcttcgccg cgaggccgac ggaaagcgtg aacgtcgtca cacgtattgc
     1321 agcggacacg acgaatttct tcgtcgtagc ggcttacgac cgtgtgcggg gtgtcggttt
     1381 tacaccgggc acagaaagcc tcgatctcct gcccgacatc atcggcttca tcatcgaaca
     1441 tcgagaagtc catcacttac cgtttcggtc tgaaaagtcg gttgaagtgg agtcatccac
     1501 atccaaccat cgtgtgcttt gtaaccgtgc ctacaagcac tcgactgagg gcatggataa
     1561 cacatcatcg tgctcggttt cctatcacgg ttttggggct ttgagcaaac aaaattcttt
     1621 ttccgcagcg atcgaaatcg gcgcctggga tgggcccgcc agaccgtgat atacggtttg
     1681 tatgcgccac gatttgcacg ccggtggaag gcccagcgtt gcttctcgtt tgcggcggtg
     1741 gctttggcaa gcaccaagcc gcgtgttggg caaaatgttg gcggcagcag gcatcgcgct
     1801 cgcggcgctc caggaaggct gttatgccgc acactacctc ggccaacagg ctggcggaca
     1861 gcttcatctt ctgagaatcc gtcggaatgt tagcgaagtc atcagtgacc caaccaccga
     1921 agaagcgctg cgccagaggc tgcaactggt catggaagcg cgaaagttcg gtatcgaaac
     1981 gctgggactg cgcggtggag ccgagtttac ccgctacgtc gatccaggcg ggccggttgc
     2041 gtacaacctg acggtgtcgc ccaaagatgc gctgacggtg cgatcatttc gttttccgct
     2101 cgttggacga gtgccctatc tgggcttctt tgatctgcgc gatgcgaagc gcgaagccca
     2161 gcgttttgcc gatgccggct ttgatgtcta tctgcgaccc gtcggcgcct attcgacgct
     2221 gggatacctg atttcgccga tttaccgttc aatgctgggt gatttttcgg cggcgggtga
     2281 gctgcgagcg gtcgagacga tcctgcatga aatggcgcac tccaccgtgt ttctccccgg
     2341 cgcgtcggat ctaaacgaga gctttgcgac ccaagtgggc attcgcggtg ccgccctctt
     2401 ttatcgcagc cggggtgacc tgacgcagag cgatcgcgtg tttgcgctgg ccaaagtagg
     2461 cgaagccgac gaaaagtcgt tttcgacctg gcttgcacca caaatcgatc ggctcaatcg
     2521 cttttacaag cgcgccgccg aggtcaagct ccgtcgcaag cagctgctcg agcagcggga
     2581 agcgattttc gaagaaatcc gtcagtcgta tcggcaggcg tttccggaag gacggcgcta
     2641 caagcgactg gcggacgggc cgctgtcgaa tgcactgctc agctcgtttg ccgtttatca
     2701 cgaaagcagc ggtcttcagg aagcgctgct ggccatctgt gacgatgatc tcaaacgatt
     2761 tgtgtcgctg tacagagaag cgcgcgatcg cagggacggc gccgagtggc tgcgtcggtt
     2821 ggcggaccta cagcgccgtt ccgttccctg atgacaggaa gctgcgacga cgaagcccgg
     2881 cataggcaag cgacgcggca acaagcccaa gagccagcag acgaattcca acgggaggta
     2941 cgtcctgcgc cgtctgcgac tgcatcgcca cttgggcccg tgcttcctgc accgcggcca
     3001 gcgcatcgac aatgccggca ccgtagtggt tgtcgtggct gccgcgatag tgaaccggtc
     3061 gtcgcgcggt ctgcttcagg atcttggcca cggcgtcggg atcgcgcaca ccttggctga
     3121 ccaccagtgc agcaacacca gcgacgtgcg gagacgccat cgacgtgccc ataaagccaa
     3181 ggtagtcctg gcgcgagatg tcacccggca cgatggtatt ttgcagcaca ccgtcgggga
     3241 ctccgtcacc gttttgatca attcgggtgt tcccgcccgg tgcggcgatg gtgatctctt
     3301 tgccccagtt tgaatagaac gtcgtcgttt cgtcgaactg ggttgcagcg acggcaatcg
     3361 ctccgcggtt ggcagcagga tagctcaccc gccctcgccc atcgttacct gccgcacaaa
     3421 cgacgacgac gccatgatca tgggcataac gaacggcgtt ggcgagcacc ggcgagttaa
     3481 acggaccgcc gaggctcatg ttgatgacct gcgccccgtg atcagcagcc cagcgaatgc
     3541 cttcggcgat gccagcgacg gaaccagagc ccgatcccga cagcactttg atcggcatga
     3601 tccgcgcgtg tggcgcgaca ccagcgacgc cgatgccgtt gtccgtggtc tgcgcgatgg
     3661 tgcccgcgac gtgggttccg tgtccgtgat cgtcctgagc gtcgggattg tcgtttacga
     3721 agttccaacc cggaacaagc tccgttccgt gtaggtcttc tactttggtg acgccggtat
     3781 cgatcacagc gacggtaaca ccctgcccac gggagagcat ccaggcttcc ggcatgttga
     3841 tctggtcaag gtgccactga tagcggtact tgggatcgct cggctgaaag cgcggttttc
     3901 cgtcgcggtc agccggtttc ggcgattcac tactggctgg aatgcgaccg gctcgatcat
     3961 ccgaggtcag cgcgccgggg gcggtttccg gaatctggta gagcagctcg agatccgcgg
     4021 cttcgaccag tggatcgtga cgaagcgcgc gcaaaagcga cgacagccgc gacgcctggt
     4081 cgccagtctt gtcgatgaac acgcgataaa tccggtcgtg cccaacgtaa gaagaatcag
     4141 cgacgaaacg aacgctgtag cgtcgggcca ggacgcggac cgtcagatca tcgacgcgat
     4201 catcgagatc caccaccaaa tcgggcagtg cttgctgact gcgaatggcc gcggcgagct
     4261 gaagctcatc gagcgagaga gcgggtgcat ccgaaagcgg ctgagccggc gaggcattcg
     4321 ccagtgcagc cgtcccaagg agcagagaac caatccacgc cgagaggcga gtctttgtca
     4381 tgatgttgtc tccacgctgc ctaccgagcc actgtgcggc tctcggcttt cagtctagca
     4441 aaaccaacca agctgcaagg gtcaaagcac ggtctacgcc cgattcccga tgccgatcat
     4501 tcattcccag cgcatggcct gcaagcgccc gtgactgcgc tgggcattgc ccaatatgaa
     4561 gtattttata tgcttcatat tgtatattct gggtgagcaa aggggtccat gcggtttcta
     4621 gttcaggacc gctgatgtcg tcgccggctt gcttcctttg gtcgcggcaa tgatcacatg
     4681 cggataggga agaacatcgg gaacgcgaac gacgcggact tgatatccca ggtcacgcag
     4741 cagatcgctc cactcattgt ggtgccggta cgtcagctcc tctttcatcc cgaccatggc
     4801 gcgatccagt atttcggtaa acttcagacc gaaccacggc ttggtcgtaa tgtcctttat
     4861 caccagcgtc ccgccttctt cgagcatgtc ggtgagctga cgcaccacat cgacctgatc
     4921 tgccgacgga atgtgatgca gcacatccag cagatagacg gcgcgaaacg gtccgcgtgc
     4981 agcagtttga cgaacatctt cggcgtaaaa ctcggtcttc accccaagcc gctcggcgac
     5041 ttttttcgcc atgtcgatgc gatgggcgtt cgggtcgatg ccgatgatgc gacggcccgg
     5101 ctgcatctgc gaaaaatacg cggaccacag accaaaacca cagccgacat ccaggatcgg
     5161 tccgtccgac ggcaagagca gatccatcgt cgcgagcagc ttcacccgca ggatcataaa
     5221 gcgaatgatg gcgtaggttc gctcgacgac cggcaacgca cgcgcgatcc gccacagggc
     5281 tttcgtggcg gacacttctt catcgtgact gggagtaaaa acgaaggacg aaggactgga
     5341 aggaggcgac gacatgcgcc gcacactaac ctcgtttttt cgcggtcgcg cgaaaaattg
     5401 ctatccgacg actggggcct tgggcggaac caggatgatc acttcacgct gatcgaggtc
     5461 ggcgcagcca ccggccagca ctttgatggt ggcgcggaaa atctgcgggc ccgtcgaagg
     5521 ctgcaccatg gtgttttgcg ccgtgaccga gaaccgcacc gtgctgcccg gatacacctt
     5581 ggtgaacgca ttgcccgaaa tcaccggagc tggcagcgtc ggcggtgcag gtggggccga
     5641 agcgtctttg ggcgtgatcg cggtgataaa gtccgccgtg gtcttgcccg ccggcagcgt
     5701 ttcacccatg tctcccgttg gatttccgga tgtctgcgtc gtcacgttga acttcgaaaa
     5761 gcgcgccacg ttggagatac cggatacaac ctgcgcatcc agacccgttc ccgccgacgg
     5821 aatcttgaag acaagcgggc attcgccgtt tgcatccgtt gcttcgccgg cgccattttg
     5881 tccggtgcag cactgacccg cagcgcagcc gactggacgc gatggaatgt cccatgctga
     5941 aggcggaacc acagcgcccg tcgaattggc catctgcacc aagtcagccg tggcgaggca
     6001 gttcgtcggc accgtcgtga cgatcgccga aacgccgatc actttggcac aaatcttatt
     6061 cagtgccgtc actgtttccg cgcgggtgtg cgccgcgttg ttcaccgatg ccaccgccga
     6121 atagccaatt tgatcggtgc caccgctggt cgtgcaggtc gtgttttcac ccttcgcgtg
     6181 gaagacggcg tccgaaatca tggtgatgac gggcagcgct cccttgcgga aaccagcgcc
     6241 gccaattcct gtggtgttcg ctggaatgtt gacgacaccg tttatgacgt ttccgacgcc
     6301 ggtcgccagc tgatacaggg cttcgatctg tccttccggc agatcgacgc cgtagccacg
     6361 cggccttgtt ccgttgagca gcaggcccac agctttcgtc gcggcggcga tatctgcggt
     6421 gatcgtctga agcagaatca gcggctgatc gtcgaggttc ggcgggaaat agttggcctg
     6481 accatacgga cttactggga agtcttcgac ggctgcaacc ccaaactggg catctttggc
     6541 agcggcggcc ttgacgcccg ggatgatggt attggtcaaa ctgttcttga gcgcctggat
     6601 ttccggaccc atgctccccg tcgtgtcgac ggcaaagaag gaatccagcg actgcacgtt
     6661 ggtgccaaac tgaagcggct gatcctggga accgcccata tacggcagct tgaagaaaaa
     6721 gtccgtcgct gcgccagtca gccgcagcca caccaccgtc aggttggcgt accccgtgac
     6781 cgtctgaccg cttgatgtcg tgtaagtcgc attcactttg gtaaagccaa cctttgccgt
     6841 cgcactctgc gccgatgtaa aggtcagacc cgctagcgaa ccgaccgccg tattttcaag
     6901 cgacagcgtc gaagaagccg atacgtcttc ggcggaacca tcgctgtagt gcgcaaagac
     6961 cgtgaacgtc ttctgcgcgg tcgtgttgac gtcgacctgc atgacgtcgt tgtcgggtga
     7021 caccgtcagg taggccagcg ttttgggctg ctccgttccc atgtcggcat tgggatcgtc
     7081 accaccgagc acggagttgc cgcagtaact catcgtgaat ccgacggtga gtgccagcga
     7141 cgaagccaga aggaagcgga atcgtggaga catggcgcgt aggcgaagca acatgatcgc
     7201 tctcctttgt cgaaggaaaa aaggacttct actttttaaa agtagttctt tgcgcggagg
     7261 aaaggaatgt ctatcgacga aaaagttgca gattgtgacc cgattgccac atcgtccgga
     7321 aaagacgcaa gccaagcggt cggatcatga acagcccgac ccaaccacca gcaacgagcc
     7381 aaaccggaga gacaacgcca aaaatccgcg gcttgccgga agttaggcgc gctgctgaag
     7441 ctctccgccg gaatcgccct gctcgtcgct tagtggcagg ggcccttcgc ggttgttgcc
     7501 gtcttcactt tggggcggcg tctcgggcgg gatttcactc tcgatgggtc gctcgggcat
     7561 gatgctttcc ttgtctgttt ggcccgctgg gttcgtgtag cgcaccttgg gattgtcctc
     7621 gggtgaggca cgtaccatga gcagattgct tgtcattttc agcatatcgc gaaccgggcc
     7681 acgacgtaaa gcgcccttga cgtaagggac gcttcgctat acacggcccc aagcaatttt
     7741 cgatgaagtg aaaacgcggt gcgactcggc aaaagcgacc gcgctcggtg gatagggaga
     7801 caacatgatc aagtggctgg ttcggctggg ctcggcggtg ggtctgcttc tcatcgtgtt
     7861 tgtgggggtc atgctgctcc tgggtggcgg cggacgagcc ctgcgtaccg aagcccgcat
     7921 cgacatcgcg cgtccagccg tggtggtgtt tccgtggctc gtcgaaggcc cgaagctgca
     7981 caagtgggtc agcggactgg agtcgtttga accccaaaac agcgagccgc aggtcggaca
     8041 aaaagtccaa atggtgctgg tccgcgacgg ccaccgctac gaagtgacgc gcgagtttac
     8101 cgcctatact ccgccgacgc ttttgcaagt acagctagaa cacagtgagt tttccgatca
     8161 aatcgagtac acgctggtcg agcacgacgg cgccacgacg ctaaccgagc gctcgacggc
     8221 gcactacaaa gacctgctct atcggctgct tgtgccgctg ctttcacgcg acacagagcg
     8281 gtcgatgaat gaaagtctcg cgacgctcaa agcgctggtc gagaccgatc cgatgccgat
     8341 tccgccgcat cctctgcctg gacaaaaggg gtttcacggc tgctgcgcgc ccgaacccga
     8401 acacaagtag cccgatccca gcaagttgcc ttgctcaaac ttgacgttac gtcagatttt
     8461 ggatacaccg ccgccgatgc tgctcctcga acacccgtat gccgaattca tctcgctcgt
     8521 cgacaaaccc gcgcgctatt tgggcggaga gtttcagtcg gtgcgcaaag actggtcgag
     8581 tgccgaggtc cgaatcgccc tcgccttccc cgacgtgtac gaaatcggga tgtcgcacct
     8641 gggcacaaag atcctctact cgctgctcag caaagatccg cgcatcctgt gcgagcgagc
     8701 gtttacgccg tgggtcgaca tggagcgcga gctgcgcaaa cgaaagctgc cgcttgtgtc
     8761 gctggaatcg gcgcgaccgc tcaaagactt cgatgtcgtt gggatgtcgc ttcagtacga
     8821 gctgacgtat accaactgcc tgacgctgct tgagctgggc gggattccgc tgcgcgctga
     8881 ggatcgcgga gaagacgatc cactggtttt ggcaggtggt cccgtcgcaa cgcatcccga
     8941 acccatcgca ccgtttttcg actgcatctt gattggcgag gctgaaaaag tcctgcctga
     9001 cgtgctgctc gactgggcgc gctggaaaaa ggaaggcatt gaccgacgcg agcgactgat
     9061 tcggctttcg cagcgcggct acctgtatgt gccgtctttg tactcggtga aaatggatgc
     9121 ggacatccat cgccaagtcg tcgaccagcc gatcgatccg cgcgttccgg ggaaggtcaa
     9181 tcgggtctac gttcccaacc tcaacgagtt tccgtttccg tcggacacgc cggttcccta
     9241 tgccgaagcg atcttcgatc gcgcgtcgat tgaaattgcc cgcggctgca ccgagggctg
     9301 ccgtttttgc caagccggga tgatctatcg tccggtgcgc gaacgcgatc ctgaatcgat
     9361 cgtcgaatcg gtggtacaga gcgtgaagcg aggcggctac gatgaaacgt cgctgacatc
     9421 gctgtcgacg gcggattttt cctgcatttt gccgctgatg aaaaaagtga tggggcggct
     9481 gcgtgaggaa cgggtgtcgc tgtcggtttc gtcgctgcgc gcctatggat tgtcgccgga
     9541 aatactcgat gaaatgtcga gcgtgcgggc gacgggtctg acgtttgcgc ccgaagcggg
     9601 cacgcagcga atgcgcgatg tcatcaacaa aaacatcacc gaagaagaca ttgcgcgctc
     9661 ggcggaaaac gtcttttcgc gtggctggtc gcgcatgaag ctgtatttca tgatcggcct
     9721 gccgacggaa accgatgaag acgtggcggg aatcattcac actgcggtgc ggctgcgcca
     9781 aatcggcaag cggattcgtc gcgacgcgga agtgaccgtc tcggtgtcaa accatgtgcc
     9841 aaaaccgcac acgccgtttc agttttgcgc gcaggacagc ctgaatgact tgcagcgcaa
     9901 acagatcctg ctgcgggaac ttgcccgtcg agaacgcatc gagttcaagt atcacgacaa
     9961 gcagatgtct tggctcgaag ggctgttttc acgcggcgac cgccggctcg cggatgtgat
    10021 tttggcggca catcgactgg gagcgcgctt cgatagctgg gacgagcagt ttgacattgg
    10081 cctgtggcac aaagcgctcg ccgaggtggg gatcgatccg acgccgtact ttatgacgat
    10141 gccgattttg gcgcggctgc cgtgggatca cctcgacatc gggctggaag aggactttct
    10201 gcgcagcgaa tatcgcaagg cgatgaagga tcgcttgtcg ccgccgtgcg gaaagcccta
    10261 cggatcgctg ctgcaccaca ccaatgtcga agatgcgctg gccgacgaaa gacggctggt
    10321 ctgcttcgac tgcggcatcg cctgtgatct gacgcagatg cggcaagaca ggctggtggc
    10381 gctgcgcaag ctcggggcgg aaacccgcac tgtggcgcca cgatcgaacg aagaagtcgc
    10441 caaagaacaa gccgagcgcg gtcagcctcg caaaaaagcg gcgttttccc aaagcgaagg
    10501 ccagcgctat cgactgcgtt atcgcaagct gggacgggcg gcgtttatcg ggcatctcga
    10561 cgcgatgcgg ctgcttcagc gcatgctgcg acgcgccggc tgcgaaccga tttatacgcg
    10621 cggcttccat cccaagcccg acatggtgtt tggtccggcg ctcggtctgg gcgtggtcag
    10681 tctcggggaa gtggtcgatc tgcgactcga gcgactttcc gaaaccgggg aatacatcca
    10741 ggccgacgag ctgcagcagc gactgcaaca ggcagcgccc gaaggctttg tcatcgtcga
    10801 agtgcagcag ctgggggccg aagacaaggc ggtttcgcgg cttatcacgg cggcggactt
    10861 tgcgattggc gtgcccaaag atgccgcgca cacagacgga gcacagcttt cgcaggttga
    10921 gtcgtgggcg cagagaccgc ttgtcgttga gcgaccagga aaagacggcg gcgagagcaa
    10981 gcgcgtcgac gtgcagcagt ttttgctgtc agcagaacac ctgggcgatc cgacttctgc
    11041 gtcggtgcgt gacgagcttg gctggcccgc ggattacgat gtggtgcgcg cgcgggttcg
    11101 tatcgacaac caaggcggcg ttcgtcccgt cgagctggcc caggcactgc ttggtgccgt
    11161 tcctgccggc gttcgctacg cgcggctcgg cctggtacta agccagtagt gcaccgtcgc
    11221 gtagggtggg cggcacgggc gctcgccgcg agttatgtca ccatgtcata atgtccagcc
    11281 ctccttccgg tgaaacgccc actcccccga tagcagatga agcgaccgca ccaacgctcg
    11341 atccagcgcg ggcgccgacc gtccatcgca gcgatcgcgg cacgcagagt gcagagcttt
    11401 tgctcgtcgc accacccggc gcaattcccg atccgctcaa acaatgggaa cgctacgatc
    11461 tagtccgtcc gctgggacaa ggcggcatgg gaacggtctt tctcgccaaa gatcgcaagc
    11521 tcggcagagc cgtcgcactc aagtttctgc gcgttcccag catcgaagcc gccgaacgac
    11581 tgctccaaga agcccgcgcc caagccagac tcgatcacaa caacatctgc aag
//
LOCUS       DAORGN010000161        11552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2334_length_11552_cov_5.600678, whole genome shotgun sequence.
ACCESSION   DAORGN010000161 DAORGN010000000
VERSION     DAORGN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_11552_cov_5.600678"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKL17_15880"
     CDS             <1..1023
                     /locus_tag="PKL17_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46264.1"
                     /translation="DLKPENIMVVPDKGAAGGERVKILDFGIAKLAMGTGQPGGMMTR
                     TGVLMGTPTYMAPEQCRGARGVDDRADVYALGVIMYQMLAGAPPFGSPGAAEVMAQHV
                     YQPPQPLQQRAPQVSTKLASLVDQMLRKQPGTRPSMAAVEKRLAAMAGLGPDPETEDP
                     SATLVFAADGPGQPPALAGTTGSEAKTHVGDPIALLPPSAPTQVAQEPTAKTQVTAES
                     PADAQTRKVAALAPPTAPTQFQDEARPRSEPLTQVNVPVVETPSPRRPNEVRQPTEDL
                     RQHAMTKLVAIVPTVFQTPPQPTLFESAAPSSRPPPSPPRTLYLSLLGALLMSVLLLV
                     LYALLG"
     gene            1151..1990
                     /locus_tag="PKL17_15885"
     CDS             1151..1990
                     /locus_tag="PKL17_15885"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNK46265.1"
                     /translation="MGAAIPLPQASAQTAPARRGEAFTFAVKMLGSLEAGRARLALSP
                     PQPSPTGPIVRVIGESEALGVAKALTGWHMSYNLILDAATLLPKRMEQVDTGKVPREA
                     TFLVNGKSFEMKVKKPGGEWQTKGELRSELLDPMSVLLLLRGVKLTDGERLSLVVSGG
                     TAVYRGELTVVGREQITTAAGPRRAIHLSGRGLRINERDEIIGQFPWIGELWLSDDAY
                     RLPLYIKAETILGMAEFSLTSYEAGSRPLAVPRNPRGITVETKSAEAKPASHDTKAPP
                     AAK"
     gene            complement(1953..2891)
                     /locus_tag="PKL17_15890"
     CDS             complement(1953..2891)
                     /locus_tag="PKL17_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNK46266.1"
                     /translation="MAKSVLIVNPQARGGFAQKKWPVLEPMLRDALGVIDVQFTKRAG
                     DGTPMAKAAIEAGADLVVAMGGDGTASEVASGMLAAFSGSARSEPSCAFGYLPCATGG
                     DLRRILHTPSELADAAAAIAKAKPRPIDAGRIDYIGHDGKPCSGYFINVASCGISGLV
                     DHLVNEGSKALGGKATFFVASLRATFRYKNALVKVRLDDQPAYEERIYTLAVSNGAYF
                     GGGMHIAPEAKIDDGIFDVTTLGDLSLVEALRLGGLIYKGEQGRLSKVSSKKARRVVI
                     EPVNPDEKVLLDVDGETPGRLPATFTLLPGALLYRG"
     gene            complement(2898..3509)
                     /locus_tag="PKL17_15895"
     CDS             complement(2898..3509)
                     /locus_tag="PKL17_15895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNK46267.1"
                     /translation="MLLEIHPSHPEPRKIARAVAALEAGEIIAYPTDTVYGLGCDLGN
                     KAAVEKLYQLKRMPKTQPLALVCVDLSDIARYVIVDNTAYRLLKRLLPGPYTFVLPAT
                     REVPKIIAPKRTQVGIRVPGHAVPRAIAAQLGRPLISTTATQFGSEPIQWPEDIAKAF
                     PQVALVLDAGPGGVVPTTVLDLTVQPHLVVRQGAGSVDFLLDE"
     gene            3689..6511
                     /locus_tag="PKL17_15900"
     CDS             3689..6511
                     /locus_tag="PKL17_15900"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK46268.1"
                     /translation="MQPESAQSTSTKIQSGEPEETSSQSASAGAAEEAEALEAGSDDG
                     GDAALDGDGADAEAPSAASPQPSYDEPPIDPKLLHLPEPSTSMRTRLRFVVPDEALTV
                     RLDPSALPEKPLDASLWDIVPKVARRALDLGLSMPENGFHIFVAADPEVMIEDVVVSH
                     AERFAAQMDTPGDLVYVHDFDHPEAPKALLLPPGTGPALVEAMSDLIDGLRERMPHLV
                     DAEDLERAHKKLSSEMEARNKQILSDLENTAKTHGFGVRTVQGAVQTFPILHGKPLSA
                     EQFDVLDETTKRALQGAADRLTREVEKAARLVRAQGARFDAEQTAAMARVAAQLIQRE
                     MRELFEHFASLSTEVARYLRRVRQALIDDWEDFLEPESDETEAEAEENLDDGDPELAT
                     RLKRFQINLLATHRPGEPAPVIYETNPTYANLYGHLERRVRFGALLTDFMRIRPGSMH
                     RSMGGVLVVRAQDLLTDPLIWERLKRVLREKRLAVEDPVGPLGLYSTTLRPAPVPMAV
                     RVVMVGSAALYDALRASDPDFGSLFRIKVEVDEVVPRSAETLPVLDAVLMSFAQKRGL
                     PPFDRSARARLLDLSTRLAEDRERLSLCMEPLDETATFAVAQARCRAIEQPKSEGKTQ
                     LVVTADDVDAAWQERRERLASEERHIRELTLRGEVALDTTGMRVGVVNGLSVFHTGDV
                     EFGQPMRITAVVALGREGIVDVEREAQLGGAIHTKGVAILRGYLARMFGQERPLSLRA
                     QLVFEQSYGEIDGDSASSSELFAVLSALADVGIDQSISVTGSVNQLGEMQAIGGVCAK
                     IEGFFDLCHARGLTGSQGVMIPKANLPHLVLRDDVVLAIQQGQFHVYAISSVAEGIEV
                     LTGLPAGERDTSGRFPAASVFGRVERRIIEIAERLRQAEAHGYDGGMDGIEDGAAEIS
                     EGGEFRRLARPISAPAASVRRLLPSR"
     gene            complement(6462..6947)
                     /locus_tag="PKL17_15905"
     CDS             complement(6462..6947)
                     /locus_tag="PKL17_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46269.1"
                     /translation="MTRSLQKNIRCALVGLLLATGLSSALATPPAGSIVVTERKLNPY
                     LGSFQQDLKTEAKKVLTKQGERDNWKVYFVAYLKKAAGDSKINVVFYDASEKVKPGQE
                     RDPVHFAEIGTKPDAKVLMSEVELRPDQGFKEGGKYQVLITRLINGKEEVYARTQLEL
                     K"
     gene            complement(6947..8125)
                     /locus_tag="PKL17_15910"
     CDS             complement(6947..8125)
                     /locus_tag="PKL17_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNK46270.1"
                     /translation="MAHPPSPTGSVSDGISATSSASPQAADEQAVVAELRDVVASLRA
                     RLKWIQHGGTDGLPMPTAKRARPPQSVAAGGGARTNLVNQSAAASAAGPIGPRPSSAP
                     VSRSAPPVDVASPDSDDPAVLARAAVENASRLAARTVLPTARPASRRPESPSAVAAAP
                     VFQSGEMNYPELVSIPRGAEGLQKIREFVGDCRRCKLCHGRNQLVFGEGNAAPLLAFV
                     GEGPGADEDRTGLPFVGAAGELLSKMIKAMGEHASKLGLPDLAARLSREQVYIGNVVK
                     CRPPGNRVPEADEIAACGPFVAAQLVALNPRVIVALGRTPTQFLLRNNAPLGRLRGQF
                     GDWNGTAVMPTYHPAYLLRTPSAKAQVWEDLKLVIMRLAEEFGHVQTSASPSLIERNP
                     "
     gene            8213..8644
                     /locus_tag="PKL17_15915"
     CDS             8213..8644
                     /locus_tag="PKL17_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNK46271.1"
                     /translation="MAITRVTDIMTEVVEALRVGDSLDLADRIMRVGRIRHLPVVDGD
                     DHLVGLVTHRTLLAAWVSHGSPGTERITDIAREIPIEMVMVKDVLTITPDAPAAEAAR
                     ILEKHKYGCLPVVGNGKLVGIITEADFLRFARIHLDQEQPS"
     gene            complement(8641..9246)
                     /locus_tag="PKL17_15920"
     CDS             complement(8641..9246)
                     /locus_tag="PKL17_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46272.1"
                     /translation="MKTENPAGHQDLGLLIDAGASATALATFLDGLDDAGRVREVRSL
                     GRKALGELYARCESAPPLTLAEFLPSSISPGKTVICAGLNNLPMFRVFEKRLVRTPSG
                     QVFGFNFQSMSFVTGPGYFAVTEAGNELLFDYTKVPSQSDVPADWPKVVPNNSGLSNF
                     VYKDLHDFCRRVSRDVVIGHATRRGDSLPQYFVLARRLDSV"
     gene            complement(9274..11127)
                     /gene="glmS"
                     /locus_tag="PKL17_15925"
     CDS             complement(9274..11127)
                     /gene="glmS"
                     /locus_tag="PKL17_15925"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNK46273.1"
                     /translation="MCGIIGYIGPKPVTSLLVEGLRRLEYRGYDSAGVAVLRETPTGD
                     CEQDLQIVRCRGKLSELEGQLAKSAPVGHVGIGHTRWATHGRPSDENAHPHRFRSVAV
                     VHNGIIENYLALKKSLTDAGHTFSSETDTEIIAHLIWAELQKVASGPDRLLRATQSAL
                     RLVEGAYAVVVLCTDTPEEIVCAKSASPLVLGLGDGENLIASDVPAILPHTRRVIFLE
                     EGDLAQLTRNRVRVIGLDGKERQRAERVITWSLLAAEKAGYKHFMLKEIHEQPRAVTD
                     TLRGRLQHAPADALLDGYELDPAKINRLFVLACGTSFHASLVGKYLIESLARIPVEVD
                     LASEFRYRDPIVKAGDLVVAVSQWGETADTLAAIREAKQKGATVFSIANVVDSSIPRA
                     SNHSLYTHAGPEIGVASTKAFTTQLLALALLALHLGRRSGQLSESAAQEFVHELTALP
                     AKLSQVLSTAEQLQQLARTYGHARDMLYLGRGPQFPIALEGALKLKEISYIHAEGYAA
                     GEMKHGPIALIDDGLPVLVLAPHDGGYEKVLSNLSEVKARGGKVIAITTEGGGAIHSL
                     ADHVVEVPAAHPLLAPILTVVPLQLFAYYVADFKGTDVDQPRNLAKSVTVE"
     gene            11299..>11552
                     /locus_tag="PKL17_15930"
     CDS             11299..>11552
                     /locus_tag="PKL17_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46274.1"
                     /translation="MSIQVIYQSLHIAKEPRFRIQDGGLFIETDSPMPVATALQVTAG
                     EHSLRGKVRRVREGAGAGMLVVPTEQVKLPRWLLGLGAEIG"
BASE COUNT         2265 a         3683 c         3491 g         2113 t
ORIGIN      
        1 gacctgaaac ccgaaaacat catggtggtg ccggacaaag gggcggcggg cggcgagcgg
       61 gtcaagattc tcgattttgg cattgcgaag ctggcgatgg ggacgggaca gccgggcggg
      121 atgatgacgc ggacgggcgt gctgatgggc acgccgacgt atatggcccc ggagcagtgt
      181 cgtggagccc gaggcgtcga cgatcgcgcc gatgtctatg ccctgggcgt catcatgtac
      241 caaatgctgg ctggggcacc gccgtttggc tcgccgggcg ctgccgaggt gatggcgcag
      301 cacgtctatc agccaccgca gccgcttcag cagcgagcac cgcaggtttc gaccaagttg
      361 gcgtcgctgg tcgatcagat gctgcgcaaa cagccgggaa cccgtccgtc gatggcagcc
      421 gtcgaaaaac gactggcggc gatggcgggg ctgggtccgg atcccgaaac cgaagacccg
      481 tcggcaacgc tggtctttgc agccgacgga ccaggccagc ccccggcact ggcaggcacg
      541 acgggatccg aagcgaaaac ccatgtcggc gatccgatcg cgctcttgcc accgagcgca
      601 ccgacgcaag tcgcccaaga accgaccgca aaaacccaag tcactgcgga atcgccagcg
      661 gatgcccaga caaggaaagt ggcagcgctc gcaccaccga cggcaccgac gcagtttcag
      721 gacgaagcac ggccacgcag cgagccgctg acccaagtca atgtcccggt ggtcgaaacg
      781 ccgtcgccgc gccgtccaaa cgaagtgcgc caaccgaccg aggatctgcg gcagcacgcc
      841 atgaccaagc tggtcgcgat cgttccgacg gtatttcaaa cgccgccgca gcccacgctg
      901 ttcgaatccg cagcgcccag ctcccggcct ccgccgtccc cgccgcggac actttacctt
      961 tcgctgctcg gcgcactgct catgtcggtg cttctgctgg tgctttatgc gctgcttggg
     1021 tgaatttcgt agcccgacag ccgccgatgc tgttagctct tgtctcgaca tgggatacgc
     1081 gaaggtacgg tctttcggca ggagcaagcg cgcttggcga ctcggctttc tgctcgcgct
     1141 cggcgtgggg atgggcgctg cgatcccact tccccaagct tcggcgcaga ccgcaccggc
     1201 tcgacgggga gaagcgttca ctttcgcggt caaaatgctc ggttcgctgg aagctgggcg
     1261 ggctcgactc gcgctgtcgc cgccgcagcc atcgccgacg ggtccgattg tccgggtcat
     1321 cggcgaaagc gaagcgctcg gcgtggccaa agccctgacc ggctggcaca tgagctacaa
     1381 cctgattctc gacgcggcga cgctgttgcc aaagcgaatg gaacaggtgg acaccggcaa
     1441 agtgccgcgc gaagccacgt ttttggtaaa cggcaagagc ttcgaaatga aggtcaaaaa
     1501 accgggcggc gagtggcaga ccaaaggcga gctgcgcagc gagctgctcg atccgatgtc
     1561 ggtgctgctt ttgctgcgcg gcgtcaagct gaccgacggg gaaaggctct cgctggtcgt
     1621 ctctggcggc acggcggttt atcgcggcga gctaaccgtc gtcggacgcg aacagatcac
     1681 gaccgcagcc ggcccaaggc gcgccattca tttgagcgga cgcggcctgc gcatcaacga
     1741 acgcgacgaa atcatcggcc agtttccgtg gatcggcgag ctgtggctca gcgacgatgc
     1801 ctaccggctg ccgctttaca tcaaggccga gacgatcctc gggatggcgg aattttcgct
     1861 caccagttac gaagccggca gtcgtccgct cgcggtgccg cgcaatccgc gtggcatcac
     1921 cgtcgaaacg aagtcagccg aagccaagcc cgctagccac gatacaaaag cgcccccggc
     1981 agcaaagtaa acgtcgccgg cagtcgtccc ggcgtctcac catccacatc gagcaggact
     2041 ttttcgtcgg gattcaccgg ttcgatgacc acccgtcggg cttttttcga cgacactttc
     2101 gacagtcggc cttgttcgcc tttgtaaatc agcccaccga ggcgcagcgc ttcaacgagc
     2161 gacagatccc cgagcgtcgt tacgtcaaag atcccgtcgt caattttggc ttcgggcgca
     2221 atgtgcatgc cgccgccaaa gtacgcgcca tttgacaccg cgagcgtgta aatccgctcc
     2281 tcatacgcag gctggtcgtc gagccgcacc ttcacgagcg catttttgta gcggaacgtg
     2341 gcccgcagcg acgcgacaaa aaacgtcgct ttgccaccga gcgctttgct gccttcgttg
     2401 accaagtgat cgaccaggcc cgatatgccg cagctggcaa cgttgatgaa gtatccgctg
     2461 cacggtttgc cgtcgtggcc gatgtagtcg attcgcccgg cgtcaatcgg ccttggcttg
     2521 gctttggcaa tcgccgccgc cgcgtcggcc agctcgctcg gggtgtggag aatccgtcgc
     2581 agatctcccc ccgttgcaca cggaagataa ccaaacgcac agcttggttc gctcctcgcg
     2641 ctgccggaaa acgccgccag catcccggaa gcaacttccg atgccgtgcc atcgcctccc
     2701 atcgcgacaa ccagatccgc gccggcttct atcgcggctt tggccatcgg tgttccgtcg
     2761 ccggctcgct tggtaaactg cacatcaatg acgccaagcg catcgcgcag catcggttca
     2821 agcaccggcc actttttctg cgcgaaaccc ccacgcgcct gcggattgac gatgagaacg
     2881 ctctttgcca tgtcggttta ctcgtccaaa agaaagtcca cgctgcccgc cccttggcgc
     2941 accaccaaat gcggctgcac cgtcagatcg agcaccgtcg tcggaaccac cccacccggc
     3001 ccagcatcca gcaccagcgc gacctgagga aacgctttcg cgatgtcttc cggccactgg
     3061 atcggctcgc tgccaaactg cgtcgcggtc gtcgagatca gcggcctgcc aagctgcgcc
     3121 gcaatcgccc gcggcaccgc atgaccgggc actcgaatgc cgacttgggt gcgcttgggt
     3181 gcgatgattt tgggcacttc tcgcgtcgct ggcagaacaa aagtataagg cccaggcaaa
     3241 agccgtttca gcagccgata cgccgtattg tcgacgatca catagcgcgc aatgtccgac
     3301 aaatcgacgc agacaagcgc cagcggctgg gtcttcggca ttcgcttgag ctgatacagc
     3361 ttttcgacgg cggctttgtt gcccagatca cagccgagtc cgtagaccgt atccgtcggg
     3421 tacgcaatga tctcacctgc ctccagcgcc gcgacggcac gggcgatctt gcggggctcg
     3481 ggatgggacg ggtggatctc cagcaacacg ctcggcagca tacactgctt gcgcgcccgt
     3541 ttcgcttctg tcaccgcgta ggccgcccct ttcgccacgc cacctaccga tcccgacaag
     3601 tgcccgtttt accgatttgt cgacggagca agtggttaaa gcatggcaat tgatcccgct
     3661 ctggtagaac atagaacctc ttttgaccat gcaacccgaa tctgcccaat ccacgagcac
     3721 caaaatacaa agcggtgaac cggaggagac atcgtcccag agcgcgtcgg ctggcgctgc
     3781 cgaggaagcc gaagcgctcg aagccgggtc ggatgacggg ggcgatgccg ccttggatgg
     3841 ggacggcgca gatgcagaag caccaagcgc agcatcgccg cagccgtctt atgacgaacc
     3901 gccgattgat cccaagctgc ttcatttgcc cgaaccgagc accagcatgc ggactcgtct
     3961 gcgttttgtg gtgcctgacg aagcgctgac ggttcggctc gatccaagtg cgctgcccga
     4021 aaagccgctc gacgcgagtc tgtgggacat cgtgcccaaa gtcgcacgcc gcgcgctcga
     4081 tctggggctt agcatgccgg aaaacggctt ccatatcttc gtcgctgccg atccggaagt
     4141 gatgatcgaa gatgtggtgg taagccacgc cgagcgcttc gccgcgcaga tggatacacc
     4201 gggcgatctg gtctacgtcc acgacttcga tcatccggaa gcgcccaaag cactgctttt
     4261 gccgccggga actggtccag cgctcgtcga agcgatgagc gatctgatcg acggtctgcg
     4321 cgaacggatg ccgcacctcg tcgatgcgga agacctggaa cgtgcgcaca aaaagctgtc
     4381 gtcggaaatg gaggcgcgca acaagcaaat cctgtccgac ctggaaaaca ccgccaagac
     4441 gcatggcttc ggggtgcgga cggtgcaagg cgcggtgcag acgttcccga tcctgcacgg
     4501 caagccgctg tcggcggaac agttcgatgt gctcgacgaa accaccaagc gagcgctgca
     4561 aggcgcggca gatcggctga cccgcgaagt cgaaaaagcg gcgcggctgg tgcgggcgca
     4621 aggggcccga tttgacgccg aacagacggc ggccatggcg cgagtggcgg cgcagctcat
     4681 ccagcgtgaa atgcgcgagc tattcgaaca tttcgccagc ttgtcgacgg aagtagcccg
     4741 ctatctgcgg cgagtgcgac aagcgctgat tgatgactgg gaagacttcc tcgagccgga
     4801 atcggacgaa acggaagccg aggccgaaga aaatctcgac gatggcgatc ccgagctggc
     4861 aacccggctc aagcgcttcc agataaacct tttggcgacg cataggccgg gtgaaccagc
     4921 gccggtgatt tacgaaacca acccgacgta tgcaaacctg tacggccacc tggaacggag
     4981 ggtgcggttt ggcgcgctgc tgaccgactt tatgcggatt cggcccggtt cgatgcatcg
     5041 ctcgatgggc ggtgtgctgg tggtccgcgc gcaggatctg ttgaccgatc cgctgatctg
     5101 ggaacggctc aagcgggtgc tgcgcgaaaa acggctggcg gtcgaagatc cggtcggtcc
     5161 gctcgggctg tattcgacga cgctgcggcc tgcaccggtt ccgatggcgg tgcgcgtggt
     5221 gatggtcgga tcggcggcgc tgtacgacgc actgcgggcg agcgatcccg actttggctc
     5281 gctgtttcgc atcaaggtcg aagtcgacga agtggtgccg cgatcggcag aaacgctgcc
     5341 ggttctcgat gccgtgctga tgtcttttgc gcaaaagcgt ggcctgccgc cgtttgatcg
     5401 ctcagcgcgg gcgcgactgc tcgatctgtc gacgcgcctg gctgaagatc gcgaacgtct
     5461 gtcgctgtgt atggaaccgc tcgatgaaac tgcgacgttt gcggtggcgc aggcacgctg
     5521 ccgggccatc gagcagccga aaagcgaagg caaaacccag cttgtggtca ctgccgatga
     5581 cgtcgatgcg gcgtggcaag aacggcggga gcgcctggcg agcgaagaac ggcacatccg
     5641 cgagctgacg ctgcgtggcg aagtggcgct cgacaccacc ggcatgcgcg tcggcgtcgt
     5701 caatggtctg tcggtctttc acaccggcga cgtcgaattc ggccagccga tgcggatcac
     5761 cgccgtcgtc gcgctcggtc gcgaaggcat tgtcgacgtc gaacgcgaag cccagctcgg
     5821 tggcgcgatt cacaccaaag gcgtcgccat tttgcgcggc tacctggccc ggatgtttgg
     5881 tcaggaacga ccgctcagct tgcgggccca gcttgtgttc gagcaaagct acggcgaaat
     5941 cgacggcgac agcgccagtt ctagcgagct atttgccgtg ctgtcggcgc ttgcggacgt
     6001 cggcatcgac cagagcatct ccgtcaccgg cagcgtcaac cagcttggcg aaatgcaggc
     6061 catcggcggc gtgtgcgcca aaatcgaagg cttcttcgac ctgtgccacg cgcgcggctt
     6121 gaccggcagc cagggcgtca tgatccccaa agccaacctg ccgcacttgg tcttgcgcga
     6181 cgatgtggtc ttggcgatcc agcagggcca gttccacgtc tacgcaattt cgtcggtggc
     6241 cgagggcatt gaagtgctca ccggactgcc cgccggcgaa cgcgatacat ccggtcgatt
     6301 ccccgccgcc agcgtgttcg gtcgcgtcga gcgccgcatc atcgaaatcg ccgagcgact
     6361 gcgccaagcc gaagcccacg gctacgacgg cggcatggat ggcatcgaag acggcgccgc
     6421 cgaaatcagc gaaggcggcg agttccgtcg cctcgcccgc cctatttcag ctccagctgc
     6481 gtccgtgcgt agacttcttc cttcccgttg ataagacggg taatcagcac ctgatacttg
     6541 ccgccttctt tgaagccctg atcggggcgc agctcgactt ccgacatcaa cactttggcg
     6601 tccggcttgg tgccgatttc cgcaaagtgc accggatcgc gctcttgccc tggtttgacc
     6661 ttttcgctgg cgtcgtagaa gaccacgttg atcttgctgt cgcccgccgc ttttttcagg
     6721 tacgcgacga aatatacttt ccagttgtcg cgctcgccct gcttggtcag cactttcttc
     6781 gcttcggtct tcagatcctg ctgaaagctg cccagatagg gattgagctt gcgctcggtg
     6841 acgacgatgc ttcccgccgg aggcgtcgcc agcgccgacg aaagtcccgt cgccagcagc
     6901 agtccaacca gggcacagcg aatgttcttt tgcaaagagc gagtcatcac ggattccttt
     6961 cgatgagcga cggcgaagcg ctggtctgca catgtccgaa ttcttcggcc aggcgcatga
     7021 tcacaagctt gaggtcttcc cagacctggg ctttcgccga cggagttcgc agaagatacg
     7081 ccgggtggta agtcggcatg accgcggtgc cgttccagtc gccaaactgc ccgcgcagac
     7141 gacccagcgg cgcgttgttg cggagcaaaa actgcgtcgg cgtccgtccc agcgcgacga
     7201 tcactcgggg gttcagcgcc acaagctgcg cggcgacaaa cggaccacag gcggcaatct
     7261 catcggcttc gggaacccga tttcccggtg gccggcactt gacgacgttg ccgatgtaga
     7321 cctgttcacg cgaaagacgc gccgccaagt cgggcaagcc cagctttgac gcatgctcgc
     7381 ccatcgcttt gatcatcttg gacagaagct ctccggcagc cccgacgaac ggcagaccgg
     7441 tgcggtcttc atcggcacct ggaccttcgc cgacaaacgc caaaagcggc gccgcgttgc
     7501 cttcgccaaa caccagttgg ttgcggccat ggcagagctt gcagcgacgg caatcaccga
     7561 caaactcacg aattttttgc aggccttcgg cgccccgcgg aatcgacaca agctcgggat
     7621 agttcatctc accactttga aaaacagggg ccgctgcgac ggcggacggc gattctggac
     7681 gccgcgatgc cggtcgcgca gtcggcagga cggtacgggc agccagacga gacgcatttt
     7741 ccaccgccgc acgcgccagc actgccgggt catcgctgtc aggagacgcc acatcgaccg
     7801 gaggcgcaga ccgggaaacc ggggcgctgg atggacgcgg accgattggc cccgcagcgc
     7861 tcgcagcggc ggactgattc acaagattcg ttcgtgcccc gccaccagca gccacgctct
     7921 gcggcggacg cgcacgcttt gcggtcggca tcggcagacc atcggtcccg ccgtgctgaa
     7981 tccacttgag ccgcgctcgc agcgacgcca caacgtctcg cagctcagcc accacggctt
     8041 gttcgtccgc tgcttgtgga ctggccgatg acgtcgcaga aatcccgtca cttacgctgc
     8101 ctgtgggcga aggtgggtgc gccatgggca gagatactaa tgcaaattcg ctgtttgcga
     8161 cgtatgttag gactactgcc cactcagatc gggccacgca ttggaggaag caatggcgat
     8221 cacccgcgtg acagacatca tgaccgaagt cgtcgaggcc ctgcgcgtcg gcgacagtct
     8281 cgatcttgcc gaccgcatca tgcgggttgg ccgcatccgt catctacccg tcgtcgacgg
     8341 agatgatcac ttggtgggac ttgtcactca ccgcacgctg ctcgccgctt gggtgagcca
     8401 cggcagcccc ggcaccgagc gcatcaccga catcgctcgc gaaattccga tcgaaatggt
     8461 gatggtcaaa gatgtgctga ccatcacgcc cgacgcgcct gccgctgaag cagcccgcat
     8521 ccttgaaaag cacaagtacg gctgtctgcc cgtcgtcggt aacggcaagc tggtcgggat
     8581 catcaccgaa gcagattttc tccgcttcgc ccgcatccac ctcgatcaag agcagccaag
     8641 ctagacgctg tcgagtcggc gcgcgagcac aaagtactgc ggcagcgaat ccccgcggcg
     8701 cgtcgcgtgc ccgatcacca catcgcgaga gacccgtcga caaaagtcgt gcaggtcttt
     8761 gtacacgaag tttgacaggc cgctgttgtt cggcaccact ttgggccaat ccgcaggcac
     8821 gtcgctttgg ctgggcactt tggtgtagtc aaaaagcagc tcgttgcccg cctcggtgac
     8881 ggcgaaataa ccggggccgg tgacaaagct catcgactga aagttgaagc caaagacctg
     8941 gccgcttggc gtgcgcacga gccgcttttc aaacacccga aacatcggca agttgttaag
     9001 ccctgcgcag atcaccgttt tgcctggcga aatcgacgac ggcaaaaact ccgccagcgt
     9061 cagtggcggg gcactttcac agcgcgcata cagttcaccg agcgctttgc gaccgagcga
     9121 gcggacctca cgcacccgcc ccgcatcgtc gagtccatcg agaaacgtgg ccagcgccgt
     9181 cgctgaagcc ccagcgtcaa tcagcaagcc gagatcctga tgtccggcgg gattttctgt
     9241 cttcatcggg tttccctctc gaaattgagc cgattattcg accgtgaccg acttggccag
     9301 gttgcgcggc tgatcgacgt cggtgccttt gaaatcggcg acgtaatagg caaaaagctg
     9361 tagcggcacg acggtcagaa tcggcgcaag cagcggatgc gccgccggaa cctcgacgac
     9421 atgatcggcc agcgaatgga tcgccccgcc tccctcggtg gtaatggcga tcactttgcc
     9481 gccgcgcgct ttgacttccg acaagtttga cagcactttt tcgtacccac cgtcgtgggg
     9541 agccagcacc agcaccggca gcccatcgtc gatcagcgca atcggcccgt gcttcatttc
     9601 gcctgccgca tagccttcgg cgtggatgta cgaaatttct ttgagcttga gcgcaccctc
     9661 gagcgcaatc ggaaactgcg gcccccgtcc cagatacagc atgtcgcgcg catgaccata
     9721 cgtccgcgcc agttgctgaa gctgttccgc cgtcgaaagc acctgcgaaa gcttggccgg
     9781 cagcgccgtc agttcatgga caaattcctg cgccgccgat tccgacaatt gaccgctgcg
     9841 ccgtcccaaa tgcagcgcca aaagcgccag cgccagaagc tgcgtcgtaa acgctttcgt
     9901 cgaggcaacg ccgatttcag gcccagcgtg ggtgtacagc gaatgattcg acgcccgcgg
     9961 aatcgatgaa tcgaccacat tggcgatcga aaacaccgtc gcgccctttt gtttggcttc
    10021 gcggatcgcc gccagcgtat cggcggtttc cccccactgc gacaccgcga cgaccaaatc
    10081 acccgctttt acgattggat cgcgatagcg gaattccgac gccagatcga cttcgacagg
    10141 aatccttgcc agcgattcga tcaggtattt tccgacgagc gacgcatgaa acgacgtgcc
    10201 gcacgccaaa acaaacagcc gattgatctt cgccggatca agctcatatc cgtcgagcag
    10261 cgcatcggca ggcgcgtgct gaagccgtcc acgcagcgta tcggtgactg cccgaggctg
    10321 ctcgtggatt tcctttagca taaagtgctt gtagccagct ttttccgccg ccaaaagcga
    10381 ccatgtgatc acccgttcgg ctcgctggcg ttcttttccg tcgagaccga tgacacgcac
    10441 ccggtttcgc gtcagctgcg ccagatcgcc ttcttcgaga aaaatcaccc gccgggtatg
    10501 cggcaaaatc gccggcacat ccgatgcgat caggttttcc ccgtcgccaa gacccagcac
    10561 aagcggcgac gcgctcttgg cgcagacgat ctcttcgggc gtatcggtac acagcaccac
    10621 gaccgcatac gctccctcga cgagccgaag cgcactctgc gtcgcccgca gcagccgatc
    10681 gggtcccgat gcgacttttt gcagctcggc ccagatcagg tgcgcaatga tttccgtgtc
    10741 ggtttccgac gaaaacgtgt ggcccgcatc cgtcagcgat ttcttcagcg ccagatagtt
    10801 ttcgatgatg ccgttgtgaa ccaccgcgac ggaacggaag cgatgaggat gagcgttttc
    10861 gtcggacgga cgaccatgcg tcgcccagcg cgtgtgcccg atgccaacgt gaccgacggg
    10921 cgccgacttc gccagttggc cttcgagttc gctcagcttg ccgcgacagc gaacgatctg
    10981 cagatcctgc tcgcagtctc ccgtcggagt ttcccgcagc accgccacgc ccgccgagtc
    11041 atagccgcga tattcaagac gccgcagccc ttcgaccaaa agcgacgtca ctggcttggg
    11101 accgatgtat ccgatgattc cgcacatagg tattctttat cccataagtt caaaggatcg
    11161 gcacggactc ggccacgaaa tgactctgtt ttcgtcggac aagtgcgtac tcgatggctg
    11221 ccatgccgcg ctggcactgg gcgattttgc gcggactgcg ttgccgccgc aaatccgctt
    11281 ttggtaactt atggaactat gagtattcag gtcatttacc agtccctgca catcgccaag
    11341 gagccgcgct tccggattca ggatggcggt ctgttcatcg aaaccgacag cccgatgcca
    11401 gtggcgacgg cactgcaagt cactgccggc gaacacagcc tgcgtggcaa agttcgtcgc
    11461 gtgcgcgaag gggctggcgc cggaatgctg gtggtgccga ccgaacaggt gaagctgccg
    11521 cgctggctgc tcggtctggg agcggaaatc gg
//
LOCUS       DAORGN010000162        11452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2377_length_11452_cov_5.995086, whole genome shotgun sequence.
ACCESSION   DAORGN010000162 DAORGN010000000
VERSION     DAORGN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2377_length_11452_cov_5.995086"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    113..212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(406..1095)
                     /gene="rpe"
                     /locus_tag="PKL17_15935"
     CDS             complement(406..1095)
                     /gene="rpe"
                     /locus_tag="PKL17_15935"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNK46275.1"
                     /translation="MNHVRIAPSILAADFGRLAEEVSAVERAGADLIHIDVMDGHFVP
                     NLTLGPPIIEALRRTTKLPLDVHLMIEQPERWIDVYAKAGADIIHVHPEVCPHLHRVV
                     QQIRHLGKQAAVVLNPATPLSVLDYVLEDLSMVLVMSVNPGFGGQSFIPSALPKIAAL
                     RKLVDERGLHDVVSISVDGGVKLDNTAEIVAAGATVLVSGSGIFATSDYGTTIAEMRH
                     RGLAACHRPQA"
     gene            complement(1126..1449)
                     /locus_tag="PKL17_15940"
     CDS             complement(1126..1449)
                     /locus_tag="PKL17_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNK46276.1"
                     /translation="MSLNKPSGAEEEYFAREEAARRQREALENAKKMADAERDAAKKL
                     HYMKCPKCGLDLKEISFKGVSIDKCFHCGGMWFDDKEFETLVGHEETNIFSSIVNVFR
                     AKKVT"
     gene            complement(1519..3543)
                     /locus_tag="PKL17_15945"
     CDS             complement(1519..3543)
                     /locus_tag="PKL17_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46277.1"
                     /translation="MDKTQPKSAAPSQPKSKPVPVATGSARPFLLRKRNSSQVLRCPD
                     LLTLRQWIAEKRLTRDDEVSRTGQKWRKMGTIVEFESLFYGVDQERLAKRKATEKDAA
                     VIPISSAAKKGVAPTPADAKTDAKTDAKIGVKADAKPAAKTEASASEKPVLGPLKVTP
                     PGLNQVSGNLSLMRLARPGSGKAKPAPTPPGLSGSAAAAKRAQDGGKTADSAAVPTAV
                     ATADTARNATAKTPAKTASPTTSDSQTAVYKRSGTDELADNDETRRIEPSDETRRIER
                     DPKTTAKTKPADPIEEAIKKSDPVPRALQEARDVTQKIPASEPDEYELIERQAARRRN
                     GILLLVAGGAALGLYYLLGSDQPTPQPTTQPVAEQKVTAPTTTPPKPVVEPLPTSNPP
                     TATAPTATAPTATAPTANANAAPVGPAPAPTVAPPAAKPAVAPVAAAPVGPAPAPTAA
                     AKPAPTVAPAPTAAPTAPTTTAAAKPTPTVTPLPPTAAAKPQVPAPAAPTAVAKPAPT
                     PAAATPAAATPAAATPAAGTKITSDEVRRITGEEIPKTFDGQIELANKYFERGKYDQA
                     LAVYQLMMTYAPNVPAIHKALGDIAFERNQADTALSHYNDSLQIRPSYSAAEFGLAKT
                     YHRLKNDKDQAISHYTKYLELNPKGSAAAACRDGITKLGGTPPAPPPAGP"
     gene            complement(3676..6660)
                     /locus_tag="PKL17_15950"
     CDS             complement(3676..6660)
                     /locus_tag="PKL17_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNK46278.1"
                     /translation="MRSADPPKPGGGPSPPNAKPAQRGSLGSLAHDDEEDRRPAPGKI
                     RLTSEGRAPVLPPSRPPAPPGPPTQPAVNLPGSSAPPGAPTVTTANMGGPAAGMGAPT
                     VRNAPSLPAPSHGGGSLANEPQGEATVMLARPEIPKDDGESTRMLSGPLPGMHFATPS
                     GGGNNESTTIGSVEQFANLPGMVKPGAASTAPPKSKLKPIAEIKAKHKVKIAPTDPQR
                     QMFQQEAAERRANENSGFLAKVKEASGLFKLSGHEPPADAPSEGTMVGQFLGFAAFAE
                     GGAGPLKDPTADTKEAVEHAPILAGLNPQFVMDAIKIGDIKLVDLGRDHLIEIQGRAL
                     LVLDGQVALARFSPQVLASEKAAQAAYVKGDKYAEKREYNRRQEVGPIIRLAESNLGL
                     FTEGDLLSLDLAGPNQVGLGVYSVTPVRALSISLTRLDTWRRTYHFFAERIRRAVDAA
                     RSRLDASTGARGMIADFFIRHGFSVGMSLRVRILSKCIECYECEKACEKRYGVKRLSL
                     GGKVLGGLDFVDCCRTCIDQRCVDVCGYDSIKFDTEKGEVVISEDLCTGCGMCSLACP
                     YDAIEMQELDEKPLLLLRLQKEKKLDWGDNKPRKAKPRRVASKCDHCASFEDQACISA
                     CPTDAIVEMSPDVAFVDRTLEQSEAAKGGYERSAFFKPGELFDPKKFFQGLSEADDNA
                     RATDKKQGGVLWTIAWIIGFIGFFLCLGEILLRRYEDLRTLSFDFWYQRRFVFPGPDE
                     VDMALNQVGYRPYDQLGLWLAYIGSTLMFSSMFYSARKWVPGIKKLGAQRGWFNYHVW
                     SGAVGPMLVLLHSAGRLDNWVSIAIWAMVAAVLSGLVGRFITTVMPERASRAELRMAD
                     LERKLTDLRNRHGGVSVADRFYQSLRQRYARVADPKMHGFVAGGLALYIYLRDVLVRP
                     FRSTLLRARLTGIKDPAARSQVASVATEMAEIECRRVLLPRIEPLFREWKLIHIPFAI
                     ILTIVGGIHIFIEAKKLLGGAM"
     gene            6879..7994
                     /gene="ruvB"
                     /locus_tag="PKL17_15955"
     CDS             6879..7994
                     /gene="ruvB"
                     /locus_tag="PKL17_15955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNK46279.1"
                     /translation="MPRKRHVDAEPAVPAQVPISGSAAHSRPSGRVADLVPAKAPHED
                     AFDAALRPRSFDEYIGQRRVVDNLKVFVEAARRRGDALDHVLFCGPPGLGKTTLAHLI
                     ASALGVTLRPVGAPAIDHKGTLASYLTSLGERDVLFIDEIHRLQPIVEEYLYPAMEDY
                     RIEIPVGEGPAAQLLHMALPRFTLVGATTRTGLLTSPLRDRFGIVLRLDYYSPVELAE
                     IVRRSALRLQVNITDAACHTIGQRARGTPRIANRLLRRVRDFAEVERDGVVADPHCGR
                     WLDRLGVDKLGLDEMDRALLRTVIERFDGGPVGIESLAAAVSEEADTIEDVYEPYLIQ
                     EGFLSRTPRGRVATRRAYEHLGLSEPVTAGPLQPRLI"
     gene            8044..9321
                     /locus_tag="PKL17_15960"
     CDS             8044..9321
                     /locus_tag="PKL17_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46280.1"
                     /translation="MGFLDDLKQGLPGVRFAIPESWKVQEQQDRRVVLLDEEHKVGWH
                     IIHAPWRADFRKDFFAEQKRDIERHARYGFEQHYHQIQVPQGTQRPPVRTTDPEFSPL
                     ISIEHVMVDGVESLFILRRVAYEPILEAVVGNIVIPLGNGLLDITVFQHSQQTGYREN
                     QLLTLALQQNPGEGLPKVRRKLGQAHFDDPANDDKFPTHPLTCVRKAVKWLLGLKKEA
                     LAVTNPQPALPSQGAEIELGQIGCAVKLPPRYLPIPDGVLPVPAGVTLLSRVILEGAD
                     DPQMLDVRQIAGVTLSPETRQAKLGEMVQQQIAEWQQNGATNIEISSEPIDPPGGSEG
                     GRVAVAVQVSMLLGGVPTHTVARWLCDTDGKVFRVGVATPPYIPTSEAAADVDLVLQS
                     FRRLPKKTGGAWLTSDLQLAPAKRAQLAAQKQA"
     gene            complement(9427..10920)
                     /locus_tag="PKL17_15965"
     CDS             complement(9427..10920)
                     /locus_tag="PKL17_15965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNK46281.1"
                     /translation="MATSKKAPTETKSKLLVHAINDERCTGCEACVLVCPTAVLELKN
                     NKSKVLRFDDCIQCEQCAVVCPTSALVMFREGTQPPPVMVPDLDDYYQSKVPGLYLLG
                     EAAGKPLVKNAVNLGRAAVEHTLRSGMRPGALSPSSRPDLTCVDVVIVGSGPAGLSAA
                     MSCQQRKLSYVVLEKDAFVASTIARYPKGKYVMAEPYDVRCVGLLPVYDSPKDQLILD
                     WNRILTKVGLDIRLREVVEKIERQQNGAFLVGTDKGKYLTQKVMMCIGTRGKPRRLNV
                     PGEDQAMVQPLLDDPDLYSGKTILVVGGGDSAVEAALSLATPQLRNKVIISYRGKAFN
                     RVKAKNRQAIDQAIHEKRVLVLFGSNVKNFGDANVVLKLVEGRERSVPIQAAFVLIGG
                     DPPIKWLEDMGIGYVQKPHGFVRPPTDMLVERLIGRQPSNDKPNLPVSDELSGFFDHA
                     ALASLGDEKKPRKEATIMVPKDQFLLHLPEKLRLDPEAQKVSQLRHH"
     gene            complement(11042..>11452)
                     /locus_tag="PKL17_15970"
     CDS             complement(11042..>11452)
                     /locus_tag="PKL17_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46282.1"
                     /translation="YHLGWDVDVQYLGATTENPLGDVRSDLFGARFAVHGQVRVPRVY
                     IRGGGGLRLGLIRNAGTPSATSDTGFGVQWGPWLTPTLVGSISVAIGRLRLDLSLEGG
                     YVLVPVRARAAGQIVAEHEGPQLLVSLGVGSFLR"
BASE COUNT         1992 a         3407 c         3609 g         2344 t
ORIGIN      
        1 gcaaaaagcg aagtcagcct catgcgaggc agacccacaa tcatggcgtg acgagtggtt
       61 tgcacggcgt gatgaggggt ttgcacggcg tgatgagggg tttgcacggc gtnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngacgaggg gtttgcacgg cgtgacgagg
      241 ggtttgcacg gcgtgacgag gggtttgcac ggcgtgacga ggggtttgta cggcgcgacg
      301 atgggtttgc acggcgcgac gaggggtttg cacggcgcga cgatgggttt gcacggcgcg
      361 acgatgggtt tgcacggcgt gatggcgacc gggcgttgcg tgccgttagg cttgcggtcg
      421 atgacaggct gcgaggccgc ggtgccgcat ctcggcgatg gttgtgccgt agtcggaggt
      481 tgcaaaaatt ccgctgcccg aaaccagcac ggtggcgccg gcagcgacga tttcagcggt
      541 gttgtcgagc tttacgccac catcgactga aatgctgacg acatcgtgaa gacctcgctc
      601 gtctaccagc ttgcgcagcg ccgcgatttt gggaagcgcg ctcgggataa acgactggcc
      661 gccaaagcct gggtttacgc tcatcacaag taccatcgac aggtcttcca gtacgtaatc
      721 gagcaccgac aaaggtgtcg ccggattgag gaccaccgcc gcctgtttgc ccagatgccg
      781 gatctgctgc acgacgcgat gcaggtgcgg acagacctcg ggatgaacgt ggatgatgtc
      841 agcgccggct ttggcataca cgtcgatcca gcgctcgggc tgttcgatca tcaggtggac
      901 gtcgagcggt agcttggtgg tccggcgcag cgcttcgatg atgggcggtc cgagcgtcaa
      961 gttgggcaca aaatgcccat ccatgacgtc gatatgaatc agatcggcgc cagcgcgctc
     1021 gacggcagac acttcctcgg cgagccgacc gaaatccgcc gcgaggatcg atggtgcgat
     1081 gcgaacgtgg ttcatggagg gccaataaca cattggccca agagatcagg tgactttctt
     1141 ggcgcggaag acgttgacga tcgacgagaa gatgttggtt tcttcgtggc cgaccagcgt
     1201 ttcgaattcc ttgtcgtcga accacatgcc gccgcagtgg aagcacttgt cgatgctgac
     1261 gcccttgaag ctgatttctt ttagatcaag tccgcacttg gggcacttca tgtagtgcag
     1321 ctttttggcg gcgtcgcgct cggcgtcagc catttttttg gcattctcaa gcgcctcacg
     1381 ctgtcgacgg gcggcttctt cgcgggcgaa gtactcttct tcggctcccg atggcttgtt
     1441 cagtgacatg gattttcctc tcgctgtggc aatgtctatc acaagctgac gtcggtgggc
     1501 aggggctgta ccgggaattt acggtccggc aggcggaggc gcaggtggcg tgccgcccag
     1561 cttggtgatg ccgtcgcgac aagccgctgc cgccgagcct tttggattga gctccaggta
     1621 tttcgtgtag tgcgaaatgg cctgatcttt gtcgtttttc agacggtgat aggtcttggc
     1681 cagaccaaat tctgcggcgg aatacgacgg gcgaatttgc agcgagtcat tgtagtgaga
     1741 aagcgcggta tcggcttggt tgcgctcgaa cgcgatgtcg ccgagcgcct tgtggatcgc
     1801 cggcacgttg ggcgcatagg tcatcatcag ctgatacacc gcgagcgcct gatcgtactt
     1861 gccgcgctcg aaatacttgt tcgccagctc gatctgtccg tcgaacgtct ttggaatttc
     1921 ttcgccggtg atgcgacgga cttcgtcgga cgtgatcttg gtgccggcag ccggtgttgc
     1981 cgcagccggt gttgccgcag ccggtgttgc cgcagcaggc gtcggggctg gtttggccac
     2041 agccgtcggt gccgcagggg ctgggacttg tggcttggct gccgcagtcg gtggaagcgg
     2101 cgtcaccgtc ggggtcggct tggctgctgc ggtcgtcgtc ggtgcagtcg gtgctgcggt
     2161 cggcgcaggt gcgaccgtcg gagcgggctt ggctgctgcc gtcggagcgg gagccggacc
     2221 gaccggagcc gccgcgactg gggccaccgc aggctttgcc gcaggcggcg cgaccgtcgg
     2281 ggctggggct gggccgactg gggccgcgtt tgcatttgcc gtcggagctg tcgccgtcgg
     2341 agcggtcgcc gttggagctg tcgccgtcgg tggattgctc gttggcagcg gttcgacgac
     2401 tggtttgggc ggtgtggtcg tcggagcggt cactttttgt tcggccaccg gctgcgtcgt
     2461 tggctggggc gtcggttgat cgctgccaag cagatagtaa agaccaagcg ccgcaccacc
     2521 tgcgacgagc agcaggatgc cattgcgtcg tcgcgccgct tgccgctcga taagctcgta
     2581 ctcgtcgggc tcactcgccg ggatcttctg cgtcacatcg cgcgcttctt gcagagcccg
     2641 tggcaccgga tcgctctttt tgatcgcttc ttcgatcgga tcggctggct tggtcttggc
     2701 ggtggtcttc ggatcgcgct caatccggcg ggtttcgtcg ctgggctcga tgcggcgggt
     2761 ttcgtcgttg tccgcaagct cgtctgtgcc gctgcgcttg tacaccgcag tttggctatc
     2821 ggaagtcgtc ggtgaagcgg tctttgccgg cgtctttgcg gtcgcgttgc gcgcggtgtc
     2881 tgccgtcgct accgcagttg gaaccgcagc gctgtcggcg gtcttgccac catcttgggc
     2941 acgctttgcc gccgcggcgc ttcccgacag acccggcggg gtgggcgctg gcttggcttt
     3001 gccacttccc ggcctggcca aacgcatcaa gctgaggttg ccgctgactt gattgagccc
     3061 cggtggtgtc accttgagcg gtccgagcac cggcttttca ctggccgaag cttcggtctt
     3121 cgccgctggt ttggcgtcgg ccttgacacc gatttttgcg tcggtttttg cgtcggtttt
     3181 tgcgtccgct ggcgtcgggg caaccccttt tttcgccgcg ctgctgatcg ggatcaccgc
     3241 cgcgtctttt tccgtcgctt tgcgcttggc cagccgttcc tggtcgacgc cgtagaacag
     3301 cgactcaaac tcgacgatgg tgcccatttt tcgccacttc tgccccgtgc gtgacacttc
     3361 gtcgtcgcgg gtcagtcgtt tttctgcgat ccactgccgc agcgtcagaa gatcggggca
     3421 gcgaagcacc tgcgagctgt ttcgcttccg caaaaggaac ggtcttgcgc ttcctgttgc
     3481 gacgggaacc ggcttgcttt tgggttgcga gggcgctgcg ctcttgggtt gggtcttgtc
     3541 catcgccatg tgtctattca ccgggcacgc gatcgtgcgg gttgcttaga gtatgccgta
     3601 tgtgattttg cagcctgatc gccgggcggg gcaagcaaat ccctgtcatg ccgaggattt
     3661 acgcggcttt gcgggctaca tcgcgccgcc taggagtttc ttcgcttcga tgaaaatgtg
     3721 gatgccaccg acgatggtca ggatgatcgc aaacggaatg tggataagct tccattcgcg
     3781 aaacagcggt tcgatgcggg gcaagagcac gcggcggcac tcgatttcgg ccatctcggt
     3841 ggcgacggaa gcgacctgac tgcgcgcagc cgggtctttg atgccggtca gtctggcgcg
     3901 cagaagcgtc gatcggaacg gacggaccag gacatcgcga agataaatgt acagcgcgag
     3961 tccgccggcg acaaagccat gcattttcgg atcagcgacg cgagcgtagc gctggcgcag
     4021 gctctggtag aagcgatcgg caacggaaac accaccgtga cggttgcgca gatcggtgag
     4081 cttgcgttcc aggtccgcca tgcgcagctc cgcgcgcgag gcgcgttccg gcatgaccgt
     4141 ggtgataaac cgtccgacga gccccgacag cactgccgca accatcgccc agatcgcaat
     4201 cgacacccag ttgtcgagcc ggcccgcgct gtgcagcagc accagcatcg gacctacggc
     4261 cccggaccag acgtgatagt taaaccagcc tcgctgcgcg ccgagctttt tgatccctgg
     4321 cacccacttg cgcgccgaat aaaacatcga cgaaaacatc agcgtgctgc cgatgtacgc
     4381 gagccacaag ccaagctgat cgtagggccg atagccgact tggtttagcg ccatgtcgac
     4441 ttcatcggga ccgggaaata cgaagcggcg ctgataccaa aaatcgaacg acagcgtgcg
     4501 caggtcttcg tagcgacgga gcaggatttc ccccaggcag aggaaaaagc cgataaagcc
     4561 gatgatccac gcgatggtcc acagcacgcc gccttgtttt ttgtcggtgg cgcgggcgtt
     4621 gtcatcggct tccgagagac cttggaagaa ctttttgggg tcgaacagct cgccgggttt
     4681 gaaaaaagcg ctgcgctcgt agccaccttt ggcagcctcg gactgttcga gcgtgcgatc
     4741 gacgaacgcg acatcgggcg acatctcgac gatggcgtcg gtcggacaag cggaaatgca
     4801 ggcctgatct tcaaagctgg cgcagtgatc gcacttgctg gcgactcggc gcggcttggc
     4861 tttgcgcggc ttgttgtcgc cccagtcgag cttcttttct ttttgcagtc gcaaaagcag
     4921 cagcggtttt tcgtcaagct cctgcatttc gatggcgtcg taagggcacg ccagcgagca
     4981 catgccgcag ccggtgcaga ggtcttcgct gatgacgacc tcgccctttt cggtgtcgaa
     5041 cttgatcgag tcgtagccgc agacgtcgac gcagcgctga tcgatacaag ttcggcagca
     5101 gtcgacaaaa tccagaccgc cgagcacttt gccaccgaga gagagccgct ttacgccata
     5161 gcgtttttca caggcttttt cgcactcgta gcactcgatg cacttgctca aaatgcggac
     5221 gcgcagcgac atgccgacgg aaaagccgtg acggatgaaa aagtcggcaa tcatgccgcg
     5281 ggcgcccgtc gacgcatcga gccgactgcg ggccgcatca acggcgcgac ggattctttc
     5341 ggcaaaaaag tgataggtcc gtcgccaggt atccagccgc gtgagcgaaa tcgacagcgc
     5401 ccgaaccggc gtcaccgaat acaccccgag cccgacttgg tttggtcccg ccaggtccag
     5461 gctcagcaga tcgccttctg taaacagacc caagttcgat tcggccagac ggatgatcgg
     5521 gccgacttcc tggcgacggt tgtattcgcg tttttcggcg tatttgtcgc ctttgacgta
     5581 ggccgcttgc gccgcttttt cgctcgccag aacctgtgga ctaaaccgcg ccagcgcgac
     5641 ttgtccatcg agtaccagca gcgcgcgacc ctgaatttcg atcaagtgat cgcgacccag
     5701 atcgaccagc ttgatgtcgc cgatcttgat cgcatccatc acaaactgcg ggttcagacc
     5761 agccaggatc ggggcatgct cgacggcttc tttggtgtcc gccgtcgggt ctttgagcgg
     5821 gccggcgcct ccttcggcaa acgcggcaaa cccgagaaac tgaccgacca tcgtgccttc
     5881 cgacggagca tcagcgggcg gttcgtgccc tgacagcttg aacagccccg acgcctcttt
     5941 gactttggca agaaagccgc tgttttcgtt tgcccgtcgt tccgccgctt cctgctgaaa
     6001 catctgccgt tgcgggtcgg tcggcgcgat cttgaccttg tgcttggcct tgatttcggc
     6061 gatcggcttg agcttgcttt tcggcggtgc tgtcgatgcg gcaccgggct tgaccatccc
     6121 cggcaggttg gcaaactgct cgacgctgcc gatggtggtt gattcgttgt tgccaccgcc
     6181 gcttggcgtc gcaaagtgca tcccaggaag cggccccgaa agcatccggg ttgattcgcc
     6241 gtcgtccttg ggaatttcgg gccgcgcgag catgaccgtc gcttcgccct gcggttcatt
     6301 tgcgaggctt cctcccccgt gtgacggcgc gggaagggat ggcgcattgc gaaccgtcgg
     6361 ggctcccatg cccgccgccg gcccgcccat gttggcggtc gtcactgtcg gggcaccggg
     6421 cggagccgaa gaccccggca gattcaccgc cggctgcgtc ggtggacccg gcggtgcggg
     6481 cggacgactc ggcggcagca cgggcgcgcg gccttcgctg gtcagacgga tcttgcccgg
     6541 cgctgggcgc cgatcctctt cgtcatcatg cgccaaactt ccgagagagc cacgctgcgc
     6601 tggttttgca tttggtgggc tcggcccgcc ccctggcttg ggcgggtcag cgctacgcaa
     6661 ggccatacgt acccccgata ccttgtgctg cggataggca gccgcaccac acaataacat
     6721 ggcatcccca agatatccga cgagaaattt tgcccggcga aaaaacctga cgctacgtta
     6781 gtttctgcca tcggaccctc gggtgagtcc gcaagacgca gcgaaatcgc ggacgggttc
     6841 gctgggtgcg gcaggggctc ctgtggcagg atgtaaccat gccacgcaaa cgccacgtcg
     6901 atgctgaacc tgcggtgccc gcgcaagtcc cgatctcggg aagtgctgcg cacagtcgcc
     6961 caagtgggcg ggtggcggat ttggtgccgg ccaaagcgcc gcacgaagat gcatttgatg
     7021 ccgcgctcag gccgcgcagc tttgacgaat acatcggtca gcgacgggtg gtggacaacc
     7081 taaaggtctt tgtcgaagcg gcccggcgac gcggagatgc gctcgatcat gtgctgtttt
     7141 gcggaccacc ggggcttggc aagaccacgc ttgcgcactt gatcgcttcg gcgcttgggg
     7201 tgacgctgcg gcccgtcggc gctccggcga tcgatcacaa aggcacgctc gcgagctacc
     7261 tgaccagctt gggggaacgc gatgtgctgt ttattgatga aattcatcgg ctccagccca
     7321 tcgtcgagga atacctgtat ccggcgatgg aggattatcg aatcgaaatt cccgtcggag
     7381 aaggtccggc tgcgcagctt ttgcacatgg cgctgccacg ctttacgctc gtcggagcca
     7441 ccacgcgcac cgggcttttg acttcgcccc tgcgcgatcg gtttggcatc gtgctgcggc
     7501 tggattacta ctcgcccgtc gagctggcgg aaattgtgcg ccgctcggcg ttgcgtctgc
     7561 aagtcaacat caccgacgct gcgtgccaca ccattgggca gcgggcgcgg ggaacgccgc
     7621 gaattgccaa tcgactgctc cggcgcgtgc gcgattttgc ggaagtcgaa cgcgatggcg
     7681 tcgtcgctga tccgcactgt gggcgctggc tcgatcggct gggcgtcgac aagctgggtc
     7741 tggatgaaat ggatcgcgcg ctgttgcgga cggtgatcga gcggtttgac ggcgggccgg
     7801 tcgggattga atcgctggcg gcagcggttt cggaagaagc cgacacgatc gaagatgtct
     7861 acgagccgta tcttatccaa gagggttttt tgtcgcgcac tccgcggggt cgcgtggcca
     7921 ctcgccgcgc ttacgaacac ctggggctgt ccgaaccggt aaccgccgga ccgcttcagc
     7981 cgcgcctcat ctgacgtttc cttgggcaca gcctttcgag gctcgtggta gagtgtggac
     8041 tccatgggct ttctagatga tttgaaacaa ggtcttcctg gggttcgttt cgccattccc
     8101 gagagctgga aggtgcaaga acagcaggac cgccgcgtcg tgcttctcga tgaggagcac
     8161 aaggtgggtt ggcacatcat ccacgcgcct tggcgggctg acttccgcaa ggacttcttc
     8221 gccgagcaaa agcgcgacat tgagcgccac gctcgctatg gcttcgagca gcactaccac
     8281 cagattcagg ttccgcaggg gacgcagcgt ccgcctgtgc gcacgaccga tccggaattt
     8341 tcgccgctga tttcgatcga gcatgtgatg gtcgacggcg tcgaaagcct gttcatcctg
     8401 cgccgggttg cctacgaacc gatcctcgag gcggtggtcg gcaacatcgt gatcccgctc
     8461 ggcaatggcc tgctcgacat cacggtgttt cagcacagcc agcagaccgg ctatcgggaa
     8521 aatcagcttc tgacgctggc gctgcagcaa aaccccggtg aggggctgcc gaaggtccgt
     8581 cgcaagctcg ggcaggcgca cttcgatgat cccgccaacg atgacaagtt cccgacgcat
     8641 ccgctgacct gcgtgcgcaa agccgtcaag tggctgcttg ggctcaagaa agaagcgctg
     8701 gcggtgacca atccgcagcc ggcgctgccg tcccaaggtg ccgaaatcga gcttggccag
     8761 atcggttgtg cggtgaagct gccgccgcgc tatctgccaa ttcccgacgg tgtgcttccg
     8821 gttcctgctg gggtgacgct gctttcgcgg gtgattttgg aaggcgccga tgatccgcag
     8881 atgctcgatg tgcggcagat cgcgggcgtg acgctgtcgc cggaaacccg tcaagccaag
     8941 cttggggaaa tggttcagca gcagattgcg gagtggcagc aaaacggtgc caccaacatc
     9001 gaaatcagct ccgagccgat cgatccaccg ggtggtagcg aaggcggtcg cgtcgcggtg
     9061 gcggtgcagg tcagcatgct gctcgggggc gttccgacgc ataccgtggc gcgctggctg
     9121 tgtgacaccg acggaaaagt cttccgggtt ggcgtggcga cgccgccgta cattccgacg
     9181 agcgaagcgg cagcagatgt cgatctggtg ctccagtcgt tccggcgctt gcccaaaaag
     9241 accggcgggg cctggctcac gtcggatctc cagctcgctc ctgccaaacg cgcccagctc
     9301 gcggcccaga agcaggccta gttttttcgg cccgcaaatc cccacgccac ccagcatcca
     9361 ccgtcgcggc gggtccaaac atggactcgc cagcgatccg atttttgctt cgaaaacccg
     9421 ctgcctttag tgatgtcgaa gctgcgagac tttttgcgct tctggatcga gccgcagttt
     9481 ttccggcagg tgcagcaaaa actggtcttt gggcaccatg atcgtcgctt ctttgcgcgg
     9541 cttcttttcg tcgccgagcg aagccagcgc cgcatgatcg aagaaacccg atagctcatc
     9601 ggacaccggc agatttggct tgtcgttcga tggctgtcgt ccaatcaggc gctcgaccag
     9661 catgtccgtc ggggggcgca caaaaccgtg cggtttttgc acatacccga tccccatgtc
     9721 ttcgagccac ttgatcggcg gatcgccacc gatcagcaca aacgccgcct gaatcgggac
     9781 gctgcgctct cggccttcga ccagcttgag caccacgttg gcgtcgccga agtttttcac
     9841 attcgagccg aacagcacga gcacgcgctt ttcgtgaatc gcctgatcaa tcgcctggcg
     9901 gttcttggct ttgacgcggt taaacgcttt gccacggtac gaaatgatga ctttattgcg
     9961 cagctgcggc gtcgccagcg aaagtgctgc ttcgacggcg ctatcgccac cgccgaccac
    10021 caggatggtt ttgccactat acagatcggg atcgtcgagc agcggctgca ccatcgcctg
    10081 atcttccccc ggcacattga gccttcgcgg cttgccacgg gttccgatgc acatcatcac
    10141 tttctgcgtg aggtatttgc ccttgtcggt gccgaccaag aatgcgccgt tttgctgccg
    10201 ttcgattttt tcgaccactt cgcgcagccg gatgtcgagc ccgaccttgg tcaagatgcg
    10261 gttccagtcg aggatgagct gatctttggg cgagtcgtag accggcaaaa gtccgacgca
    10321 gcgcacgtca tagggttcgg ccatgacgta cttgcccttg ggatagcgtg cgatcgtcga
    10381 cgccacaaac gcatcttttt ccagcacgac atacgacagc ttgcgctgct gacacgacat
    10441 cgctgccgac agacccgccg ggccgctgcc cacgatcacg acatcgacgc aggtcagatc
    10501 gggacgcgaa cttggcgaaa gcgcgcccgg acgcatgcca ctgcgcagcg tgtgctcgac
    10561 ggcggcgcga cccaggttga ccgcgttttt gaccagcggt ttgcccgcgg cttcgcccag
    10621 cagataaagc cccggcactt tcgactgata gtagtcgtcg agatccggca ccatcaccgg
    10681 cggcggctgt gttccttcgc ggaacatcac cagcgcgctc gtcgggcaga cgacggcgca
    10741 ctgttcgcac tgaatgcagt catcgaagcg cagcaccttc gatttgttgt ttttcagttc
    10801 gagcactgcc gtcggacaga ccagaacgca ggcttcgcag ccggtgcagc gttcgtcgtt
    10861 gatggcgtga accaggagct tgcttttggt ctcggtgggg gcttttttgc tcgtggccac
    10921 gcggtaaacc accagcaagg caatgaaacc aagggctatc agggggccga taatgaccgg
    10981 atccatagcc ctctattgta ccaagtccaa aatccgcgca gaacccacca aaaccaagag
    11041 atcagcgcag aaacgaaccg acaccgaggc tcaccagcag ctgtggacct tcatgttcgg
    11101 cgacgatttg tccagcagcg cgggcgcgga ccgggaccag cacatatccc ccttcaagcg
    11161 acagatcgag ccgcagcctg ccaatcgcga cactgatgct gccgaccaag gttggcgtca
    11221 gccacggtcc ccactgcacg ccaaacccgg tgtcgctggt tgcgctcggg gtgccggcat
    11281 tgcgaatcag ccccagccgc agaccgccgc cgccccgaat gtagacccgc ggcacgcgca
    11341 cctggccatg gacggcaaag cgcgcgccaa acagatcgct gcgaacgtca ccaagcgggt
    11401 tttccgtcgt cgcgcccagg tattgcacat cgacatccca gccgaggtga ta
//
LOCUS       DAORGN010000163        11440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2379_length_11440_cov_5.765657, whole genome shotgun sequence.
ACCESSION   DAORGN010000163 DAORGN010000000
VERSION     DAORGN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_11440_cov_5.765657"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1337
                     /locus_tag="PKL17_15975"
     CDS             279..1337
                     /locus_tag="PKL17_15975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46283.1"
                     /translation="MTDLKPDNVFLVPDAETAAGERTKILDFGIAKVVDPDVAEIAKT
                     STGAIVGTPIYMSPEQCRGGIAVTDRTDVYSLGIMLYQMLSGNPPFSGTGAGDLIAAH
                     IVDPPKPLREVAPQVSAEVETLVHKMLIKKPEDRPSMKQILAELEQLGLGSTGTGTQV
                     VIVQPPATAPHPAISAPLIVGVTVSIVGAIAIATILLWPKSHPVDPKPPTVPQPATQA
                     QETVSKPVETPKRVRIVLQSEPQGAAVLKVGELTPLGTTPWTTERDRSPEPLKVLLRL
                     PGYADQTEVIELGENAERTIRLTATPAPPAPPVKTNVPVQKPIAQPVAKPVTRPAKPA
                     NKPSHPAKDSVDDIPVVR"
     gene            1315..2007
                     /locus_tag="PKL17_15980"
     CDS             1315..2007
                     /locus_tag="PKL17_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46284.1"
                     /translation="MTSRWFARSVFLCAVSVSAPTLHAQEPPSPSASGAPAQSLPPPG
                     NGKDNRPLREVLDSATRHFQNSEYDQAIAEFQLAYTKRQLPTLLFNIAQAHRKAGHFS
                     EALALYERFLKDDPKSALLPEAEAHAAAMRARLDAERASAEREAAERLAKKRTEEAEA
                     LAIAREAERKKADEALLLAAKAKEPPVYKKAWFWGIIGGVVAASAITAGVVIGIKLRE
                     PASDLGVRVVDF"
     gene            2038..3798
                     /locus_tag="PKL17_15985"
     CDS             2038..3798
                     /locus_tag="PKL17_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK46285.1"
                     /translation="MRRAYLFGLWSSLCLLSLGSCSRAPTVLVSVEDIPAETASLHVM
                     PLHAGLPPHSELAPYDLPQPAPGRTTFLLRLPDSFVGDVTVEVGAYKGAGGSSCLLAT
                     GSGKLPDLQGQDTNLRVPVQPVTDTVCSGQRPLLLNATPSLGLIDGNETVQLSGWGFT
                     PSTVAQFAGKEAQTQFVSASKISALTPPRVAIGPTEINVVNKNGQSHSRGDLFRYYAS
                     TVDFGAVPFNPSSGAKNVSDVIIGNFLPKNPLVSASLVTSSKSLNIIGIVWMTPAQPI
                     PMIDNNAVSPGPGTGPSGLATADMDGDGISDVVVALSGTDQVQVFLSDGNGGLTAQPA
                     MGVQKQPEGVVASDLNGDGRPDIVSANFGADSISLLYTKPGGGFLPMGFVKTSSPAAA
                     NQPISVTVSDVNQDGIKDIAVVNQGGGNINLFIGLGGGLINPSPITLLVGTMPTQIAA
                     TDVNRDGIDDLLVVNKGSNNIQVLVNRSKKGSGTINFDTYTLATDTTPESMAFSDLNG
                     DGISDLIVPCSTTNTVNVFLNKSPDGLKGATAQKFAMPSAPVCTSVRLVAAMDIFKDG
                     QMDLAGLCTTGGGILKNQTL"
     gene            4013..6013
                     /locus_tag="PKL17_15990"
     CDS             4013..6013
                     /locus_tag="PKL17_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46286.1"
                     /translation="MAARAEVAVVGESVELRSPHSEALPLKVRLRVAATTQNDPKATP
                     PPTSVLPLTNWKRDGEDWVTEPGSKIAGLQVSVRVHGTSDGHSEISVESKATGEVWVR
                     LLSLELESESKAVDIGGRDLRPHRVKTRAALSGLDPKWVVLRPEQKPWTMLVDDEVDG
                     VKVQVLPSQVLVQADLLSSETRPFVYFQNCTNHWREPNRKAQLETRLLQPGETMTAKI
                     VAYAGAAVPLLKARYPEGRRAAFVITDHADQTTTGTLHALAGGTSLSSSPRFGKGGFL
                     GHGLLMTKSLWFRSGEPAPAPSWAHPPHPVLSPFQKNIHSLVVYHSRYERPQVDPSGA
                     GRPQLDDPAIATLAQKLHSIGWEISPHSATPQSDDRDSTDEALRFFEQYGARTWIDHQ
                     PYTNCEALTNRGFQGGRDGIADLLEQHSYRYAWSGIDVPASPQLNLLQPRRIDQYVPV
                     VYPSGRLATGTPSSLWLFSTMMTYVESSRFFGLYKKKSLDQLEQERGLHIAHTYLEAF
                     HPAGSKFARRNLMVAGKVPGEIVPHPRLEQLLVQLKGRVDRGTLWVPTMQQLGDHMRA
                     MSQVSVRLSASGSATLHAKESVTAATFVIPRPGLSVLIDGQPTKTVRHSKTETTFYVD
                     LAAGQSVKVELRDAQNRPVNLLKPTGAPTLIAKASEAASVVR"
     gene            6019..6807
                     /locus_tag="PKL17_15995"
     CDS             6019..6807
                     /locus_tag="PKL17_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46287.1"
                     /translation="MTPNTTSHGLKTPHTKPTATLQKLSRRALLLGAALTLAGIGQAA
                     CGLVSTNILDKTFELGAQKFSLDFGTTVGKVPSIACTVAGDTKCSTLGSQVSAAGGTA
                     TGNCDTNAKLCYADITVTKSYPVTLSNEPSFVSSIGSKAISVVKAIELNYGANNGSTV
                     NLPDMDLYIGPDTAKSPTDKDVYLIDKIPAIGKGVNIPDKSRKIVVQAGTPAFDRFSY
                     YLQNPKVPFSLMVVGKPTVKANDDLPSGKVDISITPAFTVGLPL"
     gene            6826..9186
                     /locus_tag="PKL17_16000"
     CDS             6826..9186
                     /locus_tag="PKL17_16000"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46288.1"
                     /translation="MRRLLALLAVLLPTLALATSGPTDGLPQPGECALWAGQARLLAR
                     RLLERAVQAEKPELARFYIETAYQLSPAPQLLYNLGLLSLRSGQSVEAADLLLRYADL
                     MGPKLPGEHKSAVSEAERSIAPAHFPLRLATSDGAFVFVDDRLVGQAPLKTPLRLSFA
                     LHRLRIELGYRQIETKISEPPPREGSDEIPTLTLRLPQALVVLAPELGAAQQKRLTAV
                     LSDQGFSLVPSRDRDLLLQQVPDRTGCLTQTGCQLWIGEQLDVEQILVVRSLDFLGDG
                     SSPKHSSRLSMEVLTVPQGSPRFRRQTLCPDCSPTRIEPYLQALLRTLRRELPDLIGE
                     SAQPGDEPAPIGFGETPGRLSPCALARGTARRLARKLLVDASQLGNASEARGRLETAY
                     QLSPSPLLLYNLGLLYRRQGQPVVAADLLARFLATADTELTSERRSKAESAMAELTGP
                     SAQLDLRGPASAQVTVDGRLHGAMPLQTPILLAPGSHVIVVESGYRTAQQELTVQAME
                     NRTLTMNLPEATLILPDEQATSRTPTLSAVARRTAEENGRIVIPDKDRELFLQSAPEY
                     AGCAASLLCMRRVGKMLGAQSVIGLLRTPRGDFAARLLDVGDSAMASAQSSECRSCDS
                     DEKSIRHVVHKLLSRQHGGRGEPLLDSKPQATLWLDGFPIGSPPQKLRLIEGTYQLEA
                     RLSPSQRLSTILRIPIDTHLTLGFVDPPTLRSRKTLAIGATLLAVGTALVATGIGVTS
                     DAGRRPPPEHNEDAKMPPGFIAGPLLLVGGAASTVAGGLVLGLRSR"
     gene            9260..9850
                     /locus_tag="PKL17_16005"
     CDS             9260..9850
                     /locus_tag="PKL17_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46289.1"
                     /translation="MTQLVGNSLPASVRSASLSRRLALRVLALCLLVLPGTLRAAAKP
                     EDILKGQLIISDSSFPLKWTSAAEYASRLKKLHKSSLFYDKKTGKLTIYYAAFFAQPV
                     NDVQVNFVIYDITNGANAKAKKGAWEAFLGRKGERALFNSVELDKEDIEMNKKYLFAI
                     ESGRQIIAKGEITLRGELPKYSGKVEFSEEDTKKKE"
     gene            9863..10834
                     /locus_tag="PKL17_16010"
     CDS             9863..10834
                     /locus_tag="PKL17_16010"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK46290.1"
                     /translation="MTSPKKACATVLLCGATWLLPRLGSAEDTAPPNRPATAEAGSTP
                     LAPPPVDEATLIAKLRSTSEAEAVAAARKLVEIKTPTVQAALYDALALGVPPKVAAAA
                     LTALAAQRSAESLPVLELYTHHRNPDLRKRATQGLAALVVAPDETDATPRPPVAGKKP
                     VKVAAKAPTIPHEELAPKVVPALIAALSDGSAEVRGVAAEALGKRREKSAESALIKLL
                     LRKDSAAPEALGQIGGPDTARALGEMTGTVPNYLITQTLGALIRRADFGPEPVRTEVV
                     KMLGKMPGAEPVDILTDYVKATETDKETKARPSRAEAQKIIEQRTAK"
     gene            10831..>11440
                     /locus_tag="PKL17_16015"
     CDS             10831..>11440
                     /locus_tag="PKL17_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46291.1"
                     /translation="MTTKRIIVGSLTLFACAQLAGCAEAAFSLHTQDNDLQTLKPAVE
                     RIKATQAAAKPLSGSGHQTVFVAVGPERKFGQRDESSQVETSLVAYDLTDRKELFSVP
                     SDIRSRFVVSQGVVMYREGANDLVLRDAQNGSVRAKVQLEPGETLAGLASDEHQLYYV
                     TRAKVGGENKSFLTAILPSGVRAWRLPGQGSMGSPAAWGGMLA"
BASE COUNT         2367 a         3526 c         3476 g         2071 t
ORIGIN      
        1 cgcaactgat ctacttgtgc gattgccttt gtcaaggctt ttgtgcgctc actagtcgat
       61 tgctgatcgt tttcgtcgac tcaccgaacg cagcccccga tatactcacg actgagtatt
      121 tacccctgat caacttccat ttcttgtcga gttccacatt gtaacgccat atcgaggatg
      181 taatcaggag cacatataaa ttttgtcctc tgtcaacaat taccagcctt ttaccacgcc
      241 atattttacg atttggcgac ggtgatgtag gtttgtggtt gacagacctg aagccggaca
      301 atgttttttt ggtgccggat gcggagacgg cagcggggga acggaccaag atcctcgact
      361 ttggcattgc caaggtggtg gacccggatg tggcagagat tgcgaagacg tcgacgggag
      421 cgattgttgg tacgccgatt tatatgtcgc cggagcagtg tcgcggcggc attgcggtca
      481 cggatcgcac ggacgtgtat tcgctcggca tcatgctgta ccaaatgcta tcgggaaacc
      541 cgccgttttc cggcaccggc gccggcgatt tgattgcggc gcacatcgtc gatcctccga
      601 agccgctgcg tgaagtcgcg ccgcaggtct ccgctgaagt ggagacgctg gtgcacaaaa
      661 tgctgatcaa aaaacccgag gatcgtccgt cgatgaaaca gatcctcgcg gagctggaac
      721 agcttgggct tggctcgacg ggaaccggaa cgcaggtggt gattgtgcag ccgccggcca
      781 ccgcgccaca ccccgcgatc tcggcaccgc ttatcgtcgg tgtgacggtc agtattgtcg
      841 gggcgattgc gattgcgacc atccttttgt ggcccaagtc ccaccccgtc gatccaaaac
      901 caccgacggt gccccagcct gccacccagg cacaggaaac cgtgagcaag cccgtcgaga
      961 cgccgaagcg ggtgcgcatc gtcctgcaga gtgaaccaca aggcgctgca gtgctaaaag
     1021 tcggcgagct gacgccgctc gggaccacgc cgtggacgac cgagcgcgat cgctcaccag
     1081 agccgctgaa ggtactgctg cgtcttccgg gctatgctga tcagacggaa gtcatcgagc
     1141 ttggcgaaaa cgccgagcga acgatccggc tgaccgccac cccagcgccc cccgcaccgc
     1201 ctgtcaaaac gaatgttccg gttcaaaaac cgattgccca gcccgtcgcc aaacccgtca
     1261 cacggccagc aaaaccggcc aacaaaccga gtcacccagc caaagactct gtcgatgaca
     1321 tcccggtggt tcgctagatc cgtctttttg tgtgcggtca gcgtttctgc gccgacgctg
     1381 catgcccagg aaccccccag ccccagcgcc agcggagcgc cggcccagtc gttgccaccg
     1441 cctggaaacg gcaaagacaa ccgcccgctg cgcgaagtgc tcgacagcgc gacgcggcac
     1501 tttcaaaaca gcgaatacga ccaggcaata gccgaattcc agctggcata taccaagcgc
     1561 cagctcccga cgctgctgtt taatatcgcg caggcacatc gcaaggcggg ccacttttcc
     1621 gaagcgctgg cgctgtacga gcggtttctc aaagacgatc cgaagtctgc gctgctcccc
     1681 gaagcggaag cgcatgcagc ggcgatgcga gcccgactcg acgccgaaag agcatcagca
     1741 gagcgcgaag ccgcggaacg cctggcgaaa aagcgcaccg aagaagccga agccttggcg
     1801 attgcccgcg aggcggaacg caaaaaagcg gacgaagcac tcttgctggc ggccaaagcc
     1861 aaagagcccc cggtttacaa aaaggcctgg ttttggggaa tcatcggcgg tgtcgtcgca
     1921 gcctctgcca tcaccgcggg ggttgtgatc gggatcaaac tgcgagagcc agcgtcggac
     1981 ttgggcgtcc gcgtcgtgga tttttagtcg cccagcgtca tgacaagggg ggcctagatg
     2041 cgtagagcgt acctgttcgg tctgtggagc agcctttgtt tgctgtcgct agggagctgt
     2101 tcgcgcgcac ccacggtttt ggtcagcgtc gaagacatcc cggcagaaac cgcgtcgctg
     2161 catgtgatgc cactgcacgc cggactgccg ccgcacagcg agcttgcccc ctacgatttg
     2221 ccgcagccag cgccggggcg aacgacgttt cttttgcgcc taccggacag ctttgtcggc
     2281 gatgtcaccg tcgaggtcgg cgcatacaaa ggggccggtg gttcatcgtg tctgctcgcg
     2341 acgggcagcg gcaagctccc tgatctgcag ggtcaggaca caaacctgcg cgtcccggtg
     2401 cagccagtga cggacacggt ctgctcgggc cagcgaccac tcctactgaa tgcgacgcca
     2461 agtctggggc tcatcgacgg caatgagacg gtgcagctga gcggctgggg gttcacgccg
     2521 tcaacggtgg cgcagtttgc tggcaaagaa gcccagacgc agtttgtctc tgcgtcgaaa
     2581 atttcggccc tgaccccgcc gcgcgtggcg atcggtccga cggaaatcaa cgtcgtgaac
     2641 aaaaatggcc aatcgcacag ccgcggcgac ttgtttcgct attacgcgag caccgtcgac
     2701 tttggcgcgg tgccgtttaa cccaagcagc ggcgcaaaga atgtgtcgga cgtcatcatc
     2761 ggcaactttt tgccgaaaaa tccgctcgtc agcgccagcc tggtcacgtc gtcgaagtcg
     2821 ctcaacatca tcggcatcgt ctggatgacc ccggcgcagc cgatcccgat gatcgacaac
     2881 aacgccgtgt cgcctggccc cggaaccggc ccaagtggtc tggccacggc agacatggac
     2941 ggcgacggca tttccgacgt agtggtcgca ctgtcgggca ccgatcaagt gcaggtcttc
     3001 ttgagcgacg gcaacggtgg cctgacggcg cagccagcga tgggtgtgca aaaacaaccc
     3061 gaaggcgtgg tcgcgtcgga tctcaatggc gacggcagac ccgacatcgt ttcagccaac
     3121 tttggggccg attcgatctc gcttttgtac accaaacctg gcggtggttt tctgccgatg
     3181 ggctttgtca aaacgtcgag cccagcagcg gcgaaccagc cgatcagcgt caccgtcagc
     3241 gacgtaaacc aggacggcat caaggacatc gcggtcgtaa accaaggcgg cggcaacatc
     3301 aatctcttca tcgggctcgg tgggggactt atcaatccgt cgccgatcac gcttttggtc
     3361 gggaccatgc ccacgcagat cgccgcaacc gatgtaaacc gcgacggcat cgacgatctg
     3421 ctcgtcgtca acaaaggcag caacaacatc caggtgctgg tcaatcggtc caaaaaaggc
     3481 agcggcacca tcaactttga tacctatacg ctggcgacgg acacgacgcc ggaatcgatg
     3541 gcgttttccg atctcaatgg cgacggcatc agcgacctga tcgtgccgtg ctcgaccacc
     3601 aacacggtga acgtgttttt gaacaagagc cccgacggac tgaaaggggc aacggcgcaa
     3661 aagtttgcaa tgccgagcgc ccccgtctgt acgagcgtcc gtctcgtcgc tgccatggac
     3721 atcttcaaag atggacaaat ggatctggcg ggcctgtgta cgaccggtgg cggaatcttg
     3781 aaaaatcaga cgctctagct gtcagaccgt cgctgtttgt ttcgttctta tataagcagc
     3841 agacccttat ataatcggag ccactccgct ctttgcggca tcttgttgat gcccatcgac
     3901 aagcggtccg agcgtaacgg tggggaagct gggtcaaagg cgctttgttt gccacgcggt
     3961 gagccagggg ctgttggtcc ttttcggact atcgtctcag cttgcagcca gcgtggcagc
     4021 ccgcgccgaa gtggcggtcg tcggcgagtc cgtcgagctg cgatcccccc acagcgaagc
     4081 gctgccactg aaggtacgac tacgggtcgc agcgacgacg caaaacgatc cgaaagcgac
     4141 accgcctccg acgagcgttt tgccgctgac caactggaaa cgcgacggtg aagactgggt
     4201 caccgaaccc ggcagcaaga tcgcaggtct tcaggtcagc gtgcgagtcc acggaaccag
     4261 cgacggacat agcgaaattt ccgttgaaag caaagccacc ggcgaagtct gggtccgtct
     4321 gctttccctg gagctggaaa gcgagtccaa agcggttgac atcggtgggc gcgatttaag
     4381 accccatcgc gtaaagaccc gcgcggcgct gtcgggtctt gatccgaagt gggtggtgct
     4441 ccgtcccgag cagaagccgt ggacgatgct tgtcgacgat gaagtcgatg gcgtcaaagt
     4501 tcaggtgctg ccgtcgcagg tgctggtcca agccgatctg ctgagcagcg aaacgcgacc
     4561 gtttgtctat tttcaaaact gcaccaacca ctggcgtgaa ccgaatcgca aagcccagct
     4621 tgagacgcga cttttgcagc ccggcgagac gatgacggcc aagatcgtcg cctacgcggg
     4681 ggcggcggtg ccgctgctca aagcccgtta tccggaaggt cgacgcgccg cgtttgtgat
     4741 caccgatcac gccgatcaaa cgacgacggg aacgctgcac gcgctggcgg gtggtacatc
     4801 gctttcatcg tcgccgcgct ttggtaaggg cggttttttg ggccacggcc tgctcatgac
     4861 caagtcgctg tggttccgca gcggcgagcc agcgccggcg ccgtcgtggg cccatccacc
     4921 gcacccggtg ctgtcgccgt ttcagaaaaa catccactcg ctggtcgtct atcacagccg
     4981 ctacgaacgt ccgcaggtcg atccatcggg agccggtcgt ccgcagcttg acgatccggc
     5041 gatcgccacg cttgcgcaaa aactgcacag catcggctgg gaaatcagtc cgcactcggc
     5101 aacgccccag tcggacgatc gcgacagcac cgacgaagcg ctgcgttttt tcgagcagta
     5161 cggggcgcgc acctggattg atcaccagcc gtacaccaac tgcgaagcgc tgacgaaccg
     5221 cggcttccaa ggcggtcgcg acgggattgc cgatctgctg gaacagcaca gctatcgcta
     5281 cgcctggtcg ggcatcgacg tgcccgcgtc accgcagcta aacctgcttc agccgcgcag
     5341 aatcgatcag tacgtcccgg tggtctatcc aagtggacgt ttggcgacgg gaacaccgag
     5401 cagtctgtgg cttttttcga cgatgatgac ctacgtcgaa agcagccgct ttttcgggct
     5461 gtataagaaa aaatcgctcg accagctgga acaagagcgc ggtctgcaca tcgcccacac
     5521 ctatctggaa gcgttccacc cggcgggcag caagttcgcg cgacgcaacc tgatggtggc
     5581 cggcaaagtg cccggcgaaa tcgtgcccca tccccgtctg gaacaactgc ttgtgcagct
     5641 gaaagggcgc gtcgatcgcg gcacgctgtg ggtgccgacg atgcagcagc ttggcgatca
     5701 catgcgtgcg atgagccaag tgtcggtgcg gctgtcggcg agcggatcag cgacgctgca
     5761 tgccaaagag tccgtgacgg cggcgacgtt tgtgattccg cgcccagggc tgtcggtgct
     5821 catcgatgga cagccgacca aaacggttcg gcacagcaag accgagacga cgttctatgt
     5881 cgatctcgcg gcgggacagt cggtcaaggt cgagctgcgc gacgcccaga accgacccgt
     5941 gaacttgcta aaaccaacgg gcgcaccgac gctgatcgcc aaagcaagcg aggcagccag
     6001 cgtggtacga taagttttat gacccctaac actacgtccc acggccttaa gaccccacac
     6061 accaaaccca ctgccacgct acaaaagctc tcgcgtcgcg cgctgctcct cggggcagca
     6121 ctcacgctgg ctggcatcgg ccaagcggcc tgtggccttg tatcgaccaa catcctcgac
     6181 aagacgttcg agctgggcgc gcagaaattc agcctcgatt ttggcaccac cgtcggcaag
     6241 gttccgtcca tcgcgtgtac cgtcgctggc gataccaagt gctccacgct gggatcgcag
     6301 gtttcggctg ctggcggaac tgcgacgggc aactgcgata caaacgccaa gctctgctac
     6361 gctgacatta ccgtcaccaa gagctaccca gtcacgctca gcaacgagcc atcgtttgtt
     6421 tcgagcatcg gcagcaaggc gatctccgtc gtaaaagcga tcgagctaaa ctacggtgca
     6481 aacaacggct cgacggtgaa cttgccggac atggatcttt acatcggtcc ggacaccgcc
     6541 aagtcgccga ccgacaaaga cgtctacctg atcgacaaaa tcccggcgat cggaaaaggc
     6601 gtaaatatcc ccgacaagtc gcgcaagatc gtcgtccagg ccggcacgcc agcgtttgat
     6661 cgcttttcgt attacctgca aaaccccaaa gtgccgtttt cgctgatggt ggtcggcaaa
     6721 ccgacggtga aagccaatga tgatctgccg tcgggcaaag tcgatatctc gatcactccg
     6781 gcgtttaccg tcgggcttcc cctgtaagga gccttggccc tgacggtgcg gcgactgctt
     6841 gcactgctcg cggtgctgct gccgacgctg gcacttgcga cgagcgggcc tacagacgga
     6901 ctcccgcaac cgggggaatg tgcgctgtgg gcaggacagg cgcggctttt ggcgcggcgt
     6961 ctgctggaac gagccgtgca ggccgaaaag cccgagcttg cgcgctttta catcgagacg
     7021 gcctatcagc tctcgccggc tccgcagctg ctttacaacc tggggctgct gtcgctgcgc
     7081 agcggccaat ccgtcgaagc tgccgatctg cttttgcgct atgccgacct gatggggcca
     7141 aagcttcccg gcgaacacaa gagcgccgtc agtgaagccg agcgatccat cgcgccagcg
     7201 cactttccac ttcgactggc taccagcgac ggtgcatttg tttttgtcga cgatcgcttg
     7261 gtcggacaag cgccgctcaa gaccccgctg cggctgtcgt ttgcgctgca ccgtctgcgc
     7321 atcgagcttg gctatcggca aatcgaaacc aaaataagcg aaccaccacc gcgcgaaggc
     7381 tccgacgaaa taccgacact gacgctgcgg ctgccgcaag ccctggtggt gctggcccct
     7441 gagcttggcg ccgcgcagca gaaacgactg acggcggtgc tcagcgatca gggtttttcg
     7501 cttgtgccgt cgcgcgatcg cgaccttttg ctccagcaag tccccgatcg caccggctgt
     7561 ttgacccaga ccggctgtca gctttggatc ggcgaacagc tcgatgtcga acaaatcttg
     7621 gtggtgcgca gcctcgattt tctcggcgat gggtcttcgc ccaaacacag ctcgcggctg
     7681 tcgatggaag tcttgacggt gccgcaagga tcgccgcggt tccgtcgcca aacgctgtgc
     7741 cccgactgta gtccgactcg catcgaaccg tatctgcaag cgctgttgcg gactttgcgt
     7801 cgcgaactgc ccgatctgat cggtgaaagc gcgcagcctg gcgacgaacc agcaccgatt
     7861 ggctttggcg aaacaccggg gcggctttcg ccgtgtgcgc tggcgcgcgg aacagcccga
     7921 cggctggcgc gaaagctgct cgtcgatgcc agccagctcg ggaacgcaag tgaagcgcgg
     7981 gggcggctgg aaacggcgta tcagctctcg ccgtcgccgc tgctgcttta caatctgggg
     8041 cttttgtatc gtcggcaagg ccagcctgtc gtcgcggctg atctgctcgc caggtttttg
     8101 gcgaccgccg acaccgaact gaccagcgag cggcgcagca aagccgaaag cgcgatggcc
     8161 gaactgaccg gaccgtcggc acagctcgat ctgcgcggcc ccgcgtcggc tcaggtcaca
     8221 gtcgatggac ggctgcatgg cgcaatgccg ctgcaaacgc cgattctgct tgcgccgggt
     8281 tcccatgtca tcgtcgtcga aagcggctat cgcacagccc agcaagagct gacggtccaa
     8341 gcgatggaaa accgcaccct gacgatgaat ctgcccgaag cgacgctgat tttgccagac
     8401 gagcaagcga cgagccgcac acccacgttg tcagcggtgg ccagacgaac cgctgaagaa
     8461 aatggccgca tcgtcattcc cgacaaagat cgcgagctgt ttttgcaaag cgcgcccgaa
     8521 tacgccggct gcgccgcgag tcttttgtgc atgcggcgcg tcggcaaaat gctcggagca
     8581 cagagcgtga taggtcttct tcggacaccg cgcggcgact ttgcggccag gctgctcgac
     8641 gtgggcgaca gtgcaatggc gagcgcgcag agcagcgagt gccgaagctg cgacagcgat
     8701 gaaaaatcga tccgccatgt ggtgcacaag ctgctgtctc gccagcacgg cggacgcggc
     8761 gagccgctgc tggatagcaa accgcaggcg acgctgtggc tcgacggttt tcccattggc
     8821 agcccgccgc agaaactgcg actcatcgaa ggcacgtatc agctggaagc gcggctgtca
     8881 ccgtcgcagc gcctgtcgac gattctgcgc atccccatcg acacccacct gacgctgggc
     8941 tttgtcgatc cgcccaccct gcgcagtcgc aagacgcttg cgatcggcgc aaccctgctg
     9001 gcggtgggga cagcccttgt cgcgacgggc atcggtgtca catccgacgc gggacgacga
     9061 ccaccaccag aacacaacga ggatgccaaa atgccccccg gtttcattgc cggaccgctt
     9121 ttgcttgtgg ggggcgcagc ctcgacggtg gccggcggtc tggttttagg tcttcgcagt
     9181 cgttgaaagc cgagccccgc tgcgtgtagc gtttcgctgc cttcttttgc ctcttcacct
     9241 ttacgaagga gacatttcga tgacccagct cgtcggcaat tccttgcctg cttccgtccg
     9301 ttccgcttcg ctgtcccgtc gcctggccct gagggttttg gcgctgtgtc ttttggtgct
     9361 gcccggcacg ctgcgggcgg cggccaaacc cgaagacatt ctgaaaggcc agctcatcat
     9421 cagcgactcg tcgtttcccc tcaaatggac gtcggcagcc gagtatgcaa gtcggctcaa
     9481 aaagcttcac aaatcgtcgc tcttttacga taagaagacc ggcaaattga ccatttacta
     9541 cgcggctttc ttcgctcagc cggtcaatga cgtgcaggtc aactttgtca tctacgacat
     9601 cacaaacggc gcaaacgcca aggcgaaaaa gggcgcatgg gaagcgttcc tgggccgcaa
     9661 gggcgagcgt gcgctgttta actccgtcga gctcgacaaa gaggacatcg agatgaacaa
     9721 gaagtacctg ttcgccatcg agtccggtcg tcaaatcatt gccaaaggcg aaatcacgct
     9781 gcgcggcgag ctgcccaagt actcgggcaa ggtcgagttt tccgaggaag atacgaaaaa
     9841 gaaagagtga gaggctgcga acatgacgag ccccaaaaag gcgtgtgcaa ccgttcttct
     9901 gtgtggcgca acgtggcttt tgcctaggct tggctcggcg gaggacaccg cgcccccgaa
     9961 tcggccagcg acggcggaag ccggaagcac gccgcttgca ccaccgcccg tcgatgaagc
    10021 gacgctcatc gccaagctgc gctccacatc ggaagctgaa gcggtcgcag ccgctcgcaa
    10081 gctcgtcgaa atcaagaccc cgaccgtgca agccgcgctg tatgacgcgc tggcgctcgg
    10141 tgtaccaccg aaagtggcag cggcggccct gacggccctg gcggcgcagc gctccgccga
    10201 atcgctgccg gtgctggaac tttacacgca ccatcgcaat cccgatctgc gcaagcgagc
    10261 gacgcaagga ctggcggcgc tggtggtggc tcccgacgaa accgatgcga ccccaaggcc
    10321 gccggtcgcg ggcaaaaagc cggtgaaagt cgcagcgaaa gcgccgacga ttccgcacga
    10381 agaactggcg cccaaagtgg taccggcgct catcgcggca ctttcggatg gcagtgccga
    10441 agtgcgcggc gtcgcggcgg aagcgctcgg caagcggcgg gaaaaatcgg cagagtcggc
    10501 gcttatcaag ctgctgcttc gcaaagactc ggcggccccc gaagcgctcg gacagatcgg
    10561 cggtccggac acggcgcgcg cgcttggtga aatgactggc acggtgccca attacctgat
    10621 tacgcagacg ctcggcgcgc tgattcggcg ggcggacttt gggccagagc cggtgcgcac
    10681 cgaagtggtc aagatgctcg gcaagatgcc aggcgcagag ccagtcgaca ttctgactga
    10741 ctacgtgaaa gcgacggaaa ccgacaaaga aacgaaggcg cgtccgtcgc gtgcggaagc
    10801 ccagaagatc atcgagcaga ggaccgccaa atgaccacaa aacgcatcat cgttggctcg
    10861 ctgactcttt tcgcgtgtgc gcagcttgcg ggctgtgcgg aagcggcgtt ttcgctgcac
    10921 acccaagaca acgacctgca gacgctgaag cccgcggtcg agaggatcaa agcgacccaa
    10981 gcggcagcga agccactgtc gggaagtggg catcagacgg tgtttgtcgc cgttggaccc
    11041 gagcgcaagt tcggtcaaag agacgagtcg agccaagtcg agacctcgct tgtcgcctat
    11101 gacctgaccg accgcaaaga gctgttttct gtcccatcgg acatccgctc gcgctttgtc
    11161 gtaagccaag gcgtggtgat gtaccgagag ggggcaaacg acctggtgct gcgcgacgcc
    11221 caaaatggca gcgtgcgcgc caaagtccag ctcgagccgg gggaaacgct ggcgggtctg
    11281 gcttctgacg aacatcagct ctattacgtg acgcgggcca aagttggcgg cgaaaacaag
    11341 agcttcctga cggcgatcct gccaagcgga gtgcgcgcgt ggcgactgcc gggtcaaggt
    11401 tcgatgggct cgccagcggc ttggggcgga atgctcgcgg
//
LOCUS       DAORGN010000164        11235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2442_length_11235_cov_5.885420, whole genome shotgun sequence.
ACCESSION   DAORGN010000164 DAORGN010000000
VERSION     DAORGN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_11235_cov_5.885420"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2733)
                     /locus_tag="PKL17_16020"
     CDS             complement(<1..2733)
                     /locus_tag="PKL17_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46292.1"
                     /translation="MRQTPRLGISGLVGPLSVVALSVATASCDVDLGSRTQLGEKTLG
                     EVVYRESCQRVAYTSQLAEHVAGTRSVVDASGVTYRPMCSDGLAAPTDAPTVMGAVSS
                     ERKNIVSGIDTAIPKSLYDDLDLALRGTMPGLEGPEAQTAAQLGGEQIRLLAESPQAT
                     AALSRLGYREGFLPRSTSGGLMRGLLAAPELHPSLTSVLPILFDSQVGAGDAPGSLSM
                     KALLSALSKEVGAISAVTSPQAPDRTLNLINRFLLTESAELVTLPAGQSYPAVRRDHR
                     GLPIVALVGTAFPSPYVDKDKDGYADISADGRFVDGAGNPIPFVTPFASISLYQKDLA
                     VARDSQGRALIAAAGQPLYSYQNLDDTVLAGLLREAPQLLDSQRDIALRLAEGASFLL
                     GPRSLDKRDYGAAGTIDYQGFDKTQSPLLDIAYSFLQLLTYSDSGATTGIELNRLLRS
                     VQLLLNNQENALARSLDAVAKAFDEAKKPDYAAAVLDEKSTLYDDLAPILVRLLRDPD
                     LLRDVLLALNDPATADLGQILALLATDSSELFMNQNQLDTRGEPADLSSVIGTFGKPV
                     DRNVPDSDVDKNPGNPKNNRSILQRVLHLVHDANQTPFCNKDGAYVNIKIVIDIKVAG
                     PAKPCTLFRLDDLALYYLLSIASDSVKMRDPYTNFLNSITDSTLKTQAQALDALGLLD
                     NLVGIPGFGKYPTPAMLARVLFQDVSKRADFFKTTLDFGACSPARPGTLCSNQNLEWQ
                     TYYDGALFGLEAVHPRDAAGNPKTNVNFYTAFRPIVDAFAKHDECIQKNASGTCIKLR
                     NAGQILVDLLAALHRHWPTAQSTFFGAPSYEPQQQKSGASRYEPLIAKLISTGDLWAS
                     TRALLPILANVLTDDGSNLPISTVIARFARFLLDPEAARIGGPLTLVSFCDMPTP"
     gene            2892..3404
                     /gene="coaD"
                     /locus_tag="PKL17_16025"
     CDS             2892..3404
                     /gene="coaD"
                     /locus_tag="PKL17_16025"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNK46293.1"
                     /translation="MSTERIAIYPGSFDPITNGHLDILQRSLKMFDKVVVALADNVRK
                     KPLFTVAERKEQILTAVGHDPRVDVDAFSGLLVSYVKARGARFVVRGLRALADFEYEF
                     QFAHMNHKLAPDVDTIFLMTAAQYFYVSSSLVKEVAQFGGDVTWMVPSGIPQALLERL
                     GAQPKLPSSA"
     gene            3440..4630
                     /locus_tag="PKL17_16030"
     CDS             3440..4630
                     /locus_tag="PKL17_16030"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNK46294.1"
                     /translation="MRISRRVEALVPSITLEVSARAQKLVAAGRDIVSLTLGEPDFAT
                     PAHVRAAACAAIERGANRYTAVSGMPELRAAAAADLSKVHGLTIQPEEVMVSTGAKQC
                     LFNALQSLIDDGDEVLIPTPTWPSHAELVRLAGGIAVLCPASASDGFCIDVDALRQKI
                     TPRTRVLLLASPSNPTGMLIDEPTMKSVVRLLHEVPDLVVLTDDLYRRLAYSPSQFVS
                     LLRLDPSLWGRVVLVDGVSKSYAMTGWRIGFCAAPRPLISAMDKLQGQVTTNAAAVSQ
                     HAALAALTGDQTPTEKMVAEFDARRRVMHRALTAIPDVRCVEPHGAFYCFPDVSAYLG
                     GTVPDDIALSRYLLEEANVAVVPGSGFFAPGHIRLSYATSVPQIEEGLRRLAHGLASI
                     RMSR"
     gene            4634..5326
                     /locus_tag="PKL17_16035"
     CDS             4634..5326
                     /locus_tag="PKL17_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46295.1"
                     /translation="MPPDAAFVSLIQTVRRWLCPGKTVLVGMIGLPGAGKSTLAKQLV
                     MALPDVAADAVSLDDFYLPRRERLARGLAHRGPPGSHDLDLLARFLDDVKSRRDKLWL
                     PVFDRGVEERATPRLCKGPLDLIVIEGFLVGIDAPGYGPLQKALSHLIFVDIDAEAAL
                     ASRRRREALLVASGQAGMTDDEVLVFWQQALWPQVEQWVLPTRARADVVVSLRSDHSL
                     QMVYFPDGTLLH"
     gene            complement(5296..7014)
                     /gene="glpD"
                     /locus_tag="PKL17_16040"
     CDS             complement(5296..7014)
                     /gene="glpD"
                     /locus_tag="PKL17_16040"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HNK46296.1"
                     /translation="MYDTSRRQAFEELQKQRFDLIVIGGGITGAGIARDAALRGLQVV
                     LVEKSDFGSGTSSKSSKLIHGGLRYLQHAEFGLVFEAVSERTLLLKLAPHLVKPQLFL
                     VPTYKGHYPGRFVLGCGMWMYDALSKFAAPDRHRAFRKKGVQQIEPTLRQDGLTGGVT
                     YYDGMTDDARLTLETLLDAQNAGAKVLSYARVRSFVEDETGAVQGVQISDVLDETSTA
                     TLLAKVTVNSTGPWSDLVLRLLRHNQPAPLVRPTKGVHIVVDAARLPVQHAVVMTVPK
                     DGRIIFAIPWTDPESSAASRTVIGTTDTDYHGDPDRIAADAADIDYLLDAANHYFPDT
                     QLVPADVLSTWAGLRPLVMPPSDGLDASSVSREHRILSRPGLLTIIGGKLTTYRRMAA
                     QLLSEAYKQLQIDEPPCATNDRPLPGAKDIAPQTDETDPVATLRDELLQKNAPGIDRQ
                     VAHHLAATYGVRARVIVDQLVQKLASGDSEAGERLDPELPFLYIEVDQAVTADLAVRL
                     DDVLSRRLPLLLLARDQGLGCVDKVCARIGNVLGWSSERRQSERLHYQDVVALSRQWR
                     SVPSGK"
     gene            complement(7072..9528)
                     /locus_tag="PKL17_16045"
     CDS             complement(7072..9528)
                     /locus_tag="PKL17_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNK46297.1"
                     /translation="MPRPVQPSSPAFAIISEEQRLWEKVANFLSTSTLPSDRKSDSHE
                     AELIELRDAIAEAKPEDLAPLVEQMTRISALAAGRRGKAVAPIDQQAPYFGHLRLRAT
                     VPANAPSRDVLIGRRGVIDRAAGIQIVDWRDAPVSQLYYRYEEGDDYDESIGQTVLQG
                     EVEVRRSITITGGKLRRISCPQGVFFCDADDVWWENESLALPTLQGGQGLSARAPKPA
                     PIQKEKEAAKTAKHEPRPEAAFVGRNDKHLPEIAALIDRHQFDLISQPQSGVVVIQGG
                     AGSGKTTVALHRVAYLHFQQPARFRGAKVMVIVPSEALVRYVSGVLPALGVSGVPVQT
                     STGWMRHHRKRLLKHLPDQQNHDTPAVVSRLKKHPGMLQLMERYVSDQADSMRRQLHD
                     AMADFPDSRKRVLAEWDARKERPLRLRCRGVRQTVSDRLEPGIGQRIDELLRRLSRRA
                     GDVWRDLGEMLTDPARLSASLGQNPDTPTTQPPVSHAEIRELIEHVNAQLEEVEELPE
                     DVDPERFQAVDGRPLDEDNPAGRLDVEDDPLLLYLYRLKFGGLDHSSGKGYVRYEHMV
                     IDEVQDLSVIEVKLLIGCVGSEDKQAGAEPSLTMAGDVAQRLIFDNGFSRWEELLPAV
                     GLRNVHIQRLNIMYRSTAEVMSLAHEVLGPLLTDESRQVATRHGAPIAAFAFTEVGEA
                     VAFLGESLRSLLQREPTASVALVARHAGTATLYYQGLQKAEVPSLRRVARHEFTFAPG
                     VDVTDVLQVKGLEFDYVVILDATGNAYPDTTESRHLLHIAITRCAHQLWLIAAGTPSP
                     LLPRHHFVKEFAPTDEPKTI"
     gene            9666..10418
                     /locus_tag="PKL17_16050"
     CDS             9666..10418
                     /locus_tag="PKL17_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNK46298.1"
                     /translation="MPSCHLVSDLPPFDAQHNLRVVVETPRGANIKIKYDEDLGCFSL
                     SRILPLGVTYPYDFGFVPQTLAQDGDPLDVMVLIDALTYPGVVISCRLLGALHVEERG
                     LRGRPNHRLVAVPVKAARKDELRNFSDLGRRMQQELERFFVMSSAFTSKDPVVRGWVG
                     PDAAFEMVQRSISRYKERQQARQQRQSGMVGGSPSGPATTTPTTPTLVQASGPASGPP
                     AGPISDTTTMPTAAATTATPTSAPIDSTVKTS"
     gene            10529..>11235
                     /locus_tag="PKL17_16055"
     CDS             10529..>11235
                     /locus_tag="PKL17_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNK46299.1"
                     /translation="MAEAPQNPVQVSEAELERMRHSTAHLLAAAVSAIWPEARFGVGP
                     AIKFGFYYDIDVPVPIGPKELERIEQKMRELRKKKLPFERIELPIGDAISEMQRRGQR
                     YKVELLTLLKDKGSTAVAKETGDESVVDGAGAATVSFYKTGEFVDLCRGPHVSVTTEI
                     GAFKLTNVAGAYWRGDEKNPQLQRVYGLAFPTQDELERHIQMLEEAKRRDHRRLGQEL
                     DLFTFSDLVGPGLPLFTP"
BASE COUNT         2087 a         3342 c         3540 g         2266 t
ORIGIN      
        1 cggcgtgggc atatcgcaaa aggacaccag tgtaagcggt ccgccgatgc gcgctgcttc
       61 cggatcgagc aaaaaccggg caaatcgggc aatcaccgtc gagatcggca gattcgatcc
      121 atcgtcggtc aagacgttcg ccaaaatcgg caaaagtgct ctcgtcgacg cccacaaatc
      181 ccccgtcgaa atcagcttgg cgatgagcgg ttcatagcgc gacgcacccg acttttgctg
      241 ctgcggctcg taagacggtg cgccaaaaaa cgtcgactgt gccgtcggcc agtgacgatg
      301 cagcgctgcc agcaggtcga ccaggatctg tcccgcattg cgcagcttga tacacgttcc
      361 gctggcattt ttttggatgc actcgtcgtg tttggcaaag gcgtcgacga tcggacggaa
      421 cgcggtgtaa aagtttacgt ttgtctttgg gttgccggcg gcgtcgcggg gatggacggc
      481 ttccagtcca aacagcgccc cgtcgtaata ggtctgccac tcgagatttt ggtttgagca
      541 cagcgtcccc ggacgcgccg gactacaggc accaaaatcg agcgtcgttt tgaaaaaatc
      601 ggcccgcttg ctgacgtcct gaaacagcac ccgcgccagc atcgccggcg tcggatattt
      661 gccaaagccc ggaatcccca ccaagttgtc gagaagcccg agcgcatcca gcgcctgcgc
      721 ctgcgtcttc agcgtcgaat cggtgatcga gttgaggaag ttggtgtacg ggtccctcat
      781 cttcaccgag tcgctggcga tcgacaaaag gtagtaaagc gccagatcat cgagccgaaa
      841 cagcgtgcac ggcttggccg ggccggcgac tttgatgtcg atgacaattt tgatgttcac
      901 gtaggcgccg tctttgttgc aaaacggcgt ctggttggcg tcgtgaacca agtgcagcac
      961 gcgctgcaaa atcgatcggt tgtttttcgg attgccggga tttttgtcga catccgaatc
     1021 gggcacattg cgatcgaccg gcttgccaaa tgtgccgatg accgacgaca agtccgctgg
     1081 ctcgccgcgg gtgtcgagct ggttttggtt cataaacagc tcgctcgaat cggtcgccaa
     1141 aagcgccaaa atctgcccca gatccgccgt cgcaggatcg tttagcgcaa gcagcacgtc
     1201 gcgcagcaga tcggggtccc gcaaaagccg aaccaaaatc ggagccagat cgtcgtacag
     1261 cgtcgatttt tcatcgagaa ctgccgccgc atagtccggt tttttcgctt catcaaacgc
     1321 cttggccacc gcgtccaggc tgcgtgccag cgcgttttcc tggttgttta ggagaagctg
     1381 cacagagcgc agcaggcgat ttagctcgat ccccgtcgtc gcgccgctgt ccgaataggt
     1441 cagaagctgc aaaaaactgt aggcaatgtc gagcagcggc gactgcgttt tgtcaaagcc
     1501 ctgatagtca atcgtccccg ccgcgccgta gtcgcgtttg tcgaggctgc gtggaccaag
     1561 tagaaagctt gcaccttcgg cgagtcgcag cgcgatgtcg cgttggctgt cgagcagctg
     1621 cggcgcttcg cggagcagcc ctgccagcac cgtgtcgtcg aggttttggt agctgtagag
     1681 cggctgtccc gccgcggcga tgagcgcgcg tccctgactg tcgcgcgcaa cggccaggtc
     1741 tttttgatac aagctgatgc tggcaaacgg cgtgacaaac ggaatcggat tgccggcacc
     1801 atcgacaaaa cgtccatcgg cgctgatatc ggcatagcca tctttgtctt tgtcgacgta
     1861 gggcgacgga aacgcggtgc ccacaagcgc gacaattggc agtccgcgat gatcgcgacg
     1921 gactgcggga tacgactgac cggcgggcag cgtgaccagt tccgccgatt ccgtcagcaa
     1981 aaaacggttt atcaggttca gtgtgcgatc aggcgcctgc gggcttgtca ccgccgagat
     2041 cgcgccgact tctttgctca gggctgaaag cagcgctttc atcgacagcg atcccggtgc
     2101 gtcgcctgcg ccaacctgcg aatcgaagag aatcggcaac accgacgtca gcgagggatg
     2161 cagctcgggc gctgccagaa gaccgcgcat aagtccaccc gacgtcgagc gtggcaaaaa
     2221 gccttcccga tagccgagcc gcgacagcgc tgccgtcgcc tggggacttt ccgccagaag
     2281 ccggatctgc tcaccgccaa gctgcgctgc cgtctgcgcc tcgggccctt ccagccccgg
     2341 catcgttccg cgcagcgcca agtcgagatc gtcgtacagc gacttgggaa tcgccgtatc
     2401 gatccccgag acaatgtttt tgcgttccga ggaaacggcg cccattacag tcggtgcatc
     2461 tgtcggggca gccagtccgt cggaacacat cggacggtac gtaacccccg acgcatcgac
     2521 gacggatcgg gttccggcga cgtgctcggc aagctgcgac gtataggcga cgcgctgaca
     2581 ggattcgcga tagaccactt ctccgagcgt cttttcgcca agctgcgtcc gactgcccag
     2641 atcgacatca cagctcgctg tcgcgacgga cagcgcgacc accgagagcg gcccgacaag
     2701 acccgaaatg ccaaggcgcg gtgtttggcg cataggacta cttgtatcgc gaaccccgtg
     2761 gtctgtcatt cgagacagca gccggtgcgg acgggtttgc aagtgccgat ctggcgatcc
     2821 ccttgactgc gagggttgct ccttgcgagg ctgcggtttt cgcagcgttt gcgaccgcgg
     2881 gagccatcat cttgtccacc gagagaatcg ccatttatcc cggctcgttc gatccaatca
     2941 ccaacggaca tttggacatc ttgcagcgct cgctcaagat gttcgacaaa gtggtggttg
     3001 cgctggccga caatgttcgc aaaaagccac tttttaccgt cgctgagcgc aaagagcaga
     3061 ttctgaccgc cgtgggtcac gatccgcggg tcgatgtcga tgcgttttcg ggactgctcg
     3121 tctcgtatgt caaagcccgc ggggcacgat ttgtcgtccg cggcctgcgc gccctcgccg
     3181 acttcgagta cgagtttcag tttgcgcaca tgaaccacaa gctcgcgccc gacgtcgaca
     3241 ccatcttctt gatgacggca gcgcagtatt tttatgtcag ctcgtcgctg gtgaaggaag
     3301 tcgcgcagtt tggtggcgac gtgacttgga tggtgccgtc ggggattccg caggcgctgc
     3361 ttgaacggtt gggggcccag ccgaagctac cgtcgtcggc gtagcggttt ttggtttcgc
     3421 tttcaaagga aggctgctca tgcgcatctc tcgtcgtgtc gaggctctgg ttccgtcgat
     3481 tacgctcgaa gtttctgcgc gggctcagaa gctcgtggcg gcggggcgcg acatcgtaag
     3541 tcttacgctc ggtgaaccgg actttgcgac ccctgcgcat gtgcgggcgg cagcctgtgc
     3601 ggcgatcgag cgaggcgcaa accgttacac cgcggtgagt ggaatgccgg agctgcgggc
     3661 ggcagcggca gccgatttgt cgaaagtgca tgggctgacg atccagcccg aagaagtgat
     3721 ggtctcgacg ggggccaagc agtgtctttt taacgcgctc cagtcgctca tcgacgacgg
     3781 agatgaagtg ctgattccga cgccgacttg gcctagccac gccgagctgg tgcggctggc
     3841 cggcggcatt gcggtgctgt gtcccgcgtc agccagcgac gggttttgca ttgatgttga
     3901 cgcgctgcgt caaaaaatca cgccgcgcac gcgggtgctg cttctggcca gtccaagcaa
     3961 tccgaccggc atgctgatcg acgaaccgac gatgaaaagc gtggttcggc tgctccatga
     4021 ggtgcctgat ctggtcgtgc tcaccgacga tctgtatcgc cggctggcct attcaccgtc
     4081 gcagtttgtg tcgcttttgc ggctggaccc gtcgctgtgg gggcgggtgg tgctggtcga
     4141 cggcgtttcc aagagctacg cgatgaccgg ctggcggatt ggcttttgtg cggcgccgcg
     4201 cccgctgatt tcggcgatgg acaagctgca aggccaggtg acgaccaatg ccgccgcggt
     4261 gtcgcaacac gccgcgctgg cggcgctgac cggggaccag acgccgacgg aaaaaatggt
     4321 tgcggaattc gatgcgcgtc ggcgggtgat gcatcgggcg ctgaccgcga ttccagatgt
     4381 gcgctgcgtc gaaccacacg gtgcgttcta ttgttttccc gatgtcagtg cttatttggg
     4441 cggaacggtt cccgacgaca ttgcgctgag ccgctatctg ctggaagaag cgaatgtcgc
     4501 ggtggttccg ggcagcggtt ttttcgctcc gggtcacatc cggctgtcgt atgcgacgtc
     4561 ggtgcctcag attgaggaag ggcttcggcg gctcgcgcac gggctggctt cgattcggat
     4621 gagccgttaa gcgatgccgc ccgatgctgc gtttgtttcg ctcatccaga cggtgcggcg
     4681 ctggctgtgt cccggcaaga ccgttttggt cgggatgatt ggcttgccgg gggcgggaaa
     4741 atcgacgctg gccaaacagc ttgtgatggc gctgcccgat gttgcggcgg atgcggtgtc
     4801 gctcgatgat ttctatctgc cgcgtcgtga gcgactggcc cgcgggctgg cccatcgcgg
     4861 accgccgggt tcgcacgatc tcgatctgct ggcgcgtttt ctcgacgatg tgaaaagtcg
     4921 gcgcgacaag ctgtggctgc cggtgttcga tcgtggcgtc gaggagcgcg cgacgccgcg
     4981 gctgtgcaaa ggtccgctcg acttgatcgt gatcgaaggc tttctcgtcg ggattgatgc
     5041 gccaggttac ggtccgctgc aaaaggcgct ttcgcacctg atcttcgtcg acatcgacgc
     5101 agaggcggcg cttgcgtcac ggcgtcggcg ggaagcgctg ctggtggcgt cgggacaagc
     5161 gggaatgacg gacgacgaag tgctggtgtt ttggcagcaa gcgctgtggc cgcaggtcga
     5221 gcagtgggtg ctgccgacgc gggcgcgggc cgacgtggtg gtgagcctgc gttccgatca
     5281 cagcttgcag atggtctatt ttcccgacgg aacgcttctc cactgacgcg acagcgcgac
     5341 gacgtcttgg tagtgcaggc gctcgctttg ccgtcgctcg cttgaccagc cgagcacgtt
     5401 gccgatgcgc gcgcagactt tgtcgacgca gccgagcccc tgatcccgcg cgagcagcaa
     5461 gagtggcagc cgacgtgaca gcacatcgtc gagccgcacc gccaagtccg cagtcacggc
     5521 ttgatcgact tcgatgtaaa gaaatggcag ctcgggatcg agccgctcgc cggcttcgct
     5581 gtccccgctg gcgagttttt gtacaagctg gtcgacgatc actctggcgc ggacgccgta
     5641 ggtggcagcc aggtgatggg cgacttgccg atcgatgccg ggcgcatttt tttgcaaaag
     5701 ttcatcgcgc agcgtcgcca ctggatcggt ttcgtccgtc tgcggtgcga tgtctttggc
     5761 tcccggcagc ggacgatcgt ttgttgcaca cggtggctcg tcgatttgga gctgtttgta
     5821 cgcttcgctc agaagctgtg ccgccatccg tcgataggtc gtcagcttgc cgccgatgat
     5881 cgtcagaaga ccgggccggc tgaggattcg gtgttcgcgc gagacactcg atgcatccag
     5941 cccgtcgctg ggcggcatca ccagcggtcg aagtcccgcc cacgtcgaaa gcacgtcggc
     6001 tgggacaagc tgggtatcgg gaaagtagtg attggcggca tcgagcagat agtcaatgtc
     6061 agcagcgtcg gcagcaatgc gatcgggatc gccgtgatag tcggtatccg ttgtgccaat
     6121 caccgtccgg ctggcggcac tcgattccgg atcggtccac gggattgcga aaatgatgcg
     6181 tccgtctttg ggcaccgtca tgacgacggc atgctgaacc ggaaggcgcg cagcatcgac
     6241 gacaatgtga acgcctttgg tgggacggac cagcggcgcc ggttggttgt gacgcaaaag
     6301 ccgcagcacc agatccgacc acggtccggt cgaattgacg gtgactttgg ccaacagcgt
     6361 cgccgtcgat gtttcatcga gcacatcgct gatttgcacg ccttgcaccg cgccggtttc
     6421 atcttcgacg aagctgcgca cgcgcgcata cgacagcact ttcgcccctg cgttttgtgc
     6481 atcgagcagc gtttccaaag tcagccgcgc gtcgtcggtc atcccgtcgt aatacgtcac
     6541 gccgccggtc agaccgtctt ggcgcagggt tggttcgatt tgctgtacgc cttttttgcg
     6601 gaaggcgcgg tgacgatcgg gcgcggcaaa cttcgacagc gcgtcgtaca tccacatgcc
     6661 gcagccgagc acaaaccgcc ccggatagtg gcctttgtac gtcggaacca aaaacagctg
     6721 cggcttgacc aggtgcggtg ccagcttgag cagaagcgtt cgttcgctca ccgcctcgaa
     6781 gaccagacca aactcggcat gctgcaaata gcgcagaccg ccgtggatca gcttcgatga
     6841 tttgctgctc gtgcctgatc caaagtcgct tttttcgacg agcaccactt ggagcccacg
     6901 cagcgctgca tcgcgcgcaa tccccgcccc ggtgatgccg ccgccgatga cgatgagatc
     6961 gaagcgctgc ttttgaagct cctcaaacgc ttggcgccga gaagtgtcgt acatgttcca
     7021 ttccttcagt aaccgacagc aggaaaaaaa cccagcactt cattaggtgc tctagatggt
     7081 cttcggttcg tcggttggcg caaactcttt gacgaagtgg tggcgcggca gaagcggtga
     7141 cggggtccct gccgcaatca gccacagttg gtgcgcacag cgtgtgatcg caatgtgcag
     7201 aaggtgacgc gactcggtgg tatcgggata agcatttccc gtcgcgtcca gaatcaccac
     7261 gtagtcaaac tcgagacctt tgacctgcag cacgtcggtc acatcgacgc cgggcgcaaa
     7321 ggtaaattcg tgtcgagcaa cccgacgcag cgacgggact tcggcttttt gcaggccctg
     7381 ataataaagc gtcgccgtcc ccgcgtggcg cgcgaccaag gccaccgacg cggttggttc
     7441 gcgctggagc aggctgcgca gcgattcccc gaggaaggcc accgcttccc cgacttcggt
     7501 aaaggcaaat gctgcgatcg gtgcaccatg ccgggtcgcc acctgtcgac tttcgtccgt
     7561 gagcagcgga cccagcactt catgcgccag cgacatcact tccgccgtcg agcgatacat
     7621 gatgttgagt cgctgaatgt ggacattgcg cagccccacc gccggcaaca gctcttccca
     7681 gcgcgaaaaa ccgttgtcga agatcagccg ctgcgcgacg tcacctgcca tcgtcagcga
     7741 cggttccgcg ccggcttgct tgtcttctga accgacgcag ccaatcagca gcttgacctc
     7801 gatgaccgac agatcctgca cttcgtcgat gaccatgtgc tcgtaacgga catagccttt
     7861 gcccgacgag tgatcgagac cgccaaactt gagccggtac agatacaaaa gcagcggatc
     7921 gtcttcgaca tcgagccgcc ccgccgggtt gtcttcatcg agcggacgac catccaccgc
     7981 ctggaaacgc tccggatcga cgtcttcggg cagctcctcg acctcttcga gctgagcatt
     8041 gacatgctcg ataagttccc gaatctcggc gtggctcact ggcggttgcg tcgtcggagt
     8101 gtcgggattt tggccaagac ttgccgaaag ccgcgcgggg tcggtcagca tttcgcccag
     8161 atcgcgccac acatcgcctg cccgtcgaga aagccgtcgc aaaagttcat ctatgcgctg
     8221 gccgatgcct ggttccagcc gatccgacac cgtctgccga accccacgac agcgcagccg
     8281 cagcggtctt tcttttcgcg catcccactc ggcgagcact cgcttgcggc tgtcggggaa
     8341 atccgccatc gcatcgtgaa gctgccgtct catgctgtcg gcttggtcgc tcacgtagcg
     8401 ttccatcagc tggagcatgc ctgggtgttt tttgagccgc gacaccacgg caggcgtgtc
     8461 gtgattttgc tggtcgggca ggtgtttgag cagccgcttg cgatggtggc gcatccagcc
     8521 ggtgctcgtc tgcaccggga cgcctgacac cccgagcgcc ggcagcacgc cgctgacata
     8581 gcgaaccagc gcttcgctcg ggacaatcac catcactttg gcgccgcgga agcgcgcggg
     8641 ctgctgaaag tgaagatagg ccacccggtg cagcgcgacg gtggttttgc cgctgccagc
     8701 gccgccctga atgacgacga caccgctttg cggctggctt atcaaatcga actggtggcg
     8761 atcgataagc gcggcaattt caggcagatg tttgtcgttg cgaccgacga atgcggcttc
     8821 gggacgcggt tcgtgtttgg cggttttggc cgcttctttt tctttctgaa tcggcgcagg
     8881 tttgggcgcg cgtgccgaca gaccttgccc gccttgcagc gtcggcagtg ccagcgattc
     8941 gttttcccac cagacatcat cggcgtcaca gaaaaaaacg ccctgcggac agcttatgcg
     9001 acgtagcttt ccgccggtga tggtgatgct gcgacggacc tcgacttcac cttggagcac
     9061 cgtctggccg atgctttcgt cgtagtcgtc gccttcttcg tagcgatagt aaagctgcga
     9121 caccggcgca tcacgccagt cgacgatctg gatgccggcg gcgcgatcga tgaccccgcg
     9181 tcgcccgatc agcacatccc ggctcggcgc gttggctggt actgtcgctc ggagccgcaa
     9241 gtgaccaaaa tagggcgcct gctgatcgat gggggcgacg gcttttccgc gtcggcccgc
     9301 ggccagggcg ctgatgcgcg tcatttgctc gacgagcggc gccaagtctt cgggtttggc
     9361 ttctgcaatc gcgtcgcgca gttcgataag ctcggcctcg tggctgtcgc ttttgcgatc
     9421 cgatggcagc gtcgaagtac tgaggaagtt ggcgaccttt tcccagagcc gctgctcttc
     9481 cgaaatgatg gcaaaagcgg gcgaggaagg ctggactggc cgaggcatgg ggtgcgcagt
     9541 atgcctggca agccgatacg cggcaacggc agccgacaaa aagactgcaa ctgcgcgaaa
     9601 gatgacgagg gcaccgctgt gtgcgtggta gactgaactt tccccacctg gagtgggtta
     9661 ccgccatgcc ttcgtgccac ttggtttctg atctgccgcc ctttgacgcc cagcacaacc
     9721 tgcgtgtggt cgtcgagacc ccacgggggg cgaacatcaa gatcaagtac gacgaagacc
     9781 tgggctgctt ttcgctcagt cggattttgc cgctcggcgt gacctatccc tacgactttg
     9841 gctttgtccc gcagacgctg gcgcaagatg gcgatccgct cgatgtgatg gtgctgatcg
     9901 atgcgctgac ctatccgggc gtggtgattt cgtgtcggct gctcggggcg ctgcacgtcg
     9961 aagaacgcgg tctgcgcggg cgacccaatc atcggcttgt cgcggtgcca gtcaaagcgg
    10021 cgcgcaaaga cgagctgcgc aacttttccg acctgggacg acggatgcag caggagctgg
    10081 agcgcttctt tgtgatgtcg tcggcgttta ccagcaaaga tccggtggtg cgcggctggg
    10141 ttggtcccga cgctgcgttc gaaatggtgc agcgctcgat cagtcgatac aaagaacggc
    10201 agcaagcgcg gcagcagcgt cagagcggca tggtgggggg aagtccatcg ggtccagcga
    10261 cgacgacgcc gacgacgccg actctggttc aggccagcgg tccagcgagc ggcccaccag
    10321 ccggcccgat aagcgacacg acgacgatgc cgacggcggc tgcgacgaca gcgacgccga
    10381 cgagcgctcc gatcgattcc accgtcaaga cttcgtaagc tcgatcgcgg cggcggcgtc
    10441 gcggttgaga atttgcccgt ctcgcggtac taaatacctc cgcgcaaaac ggacttgcgc
    10501 gcacaagcag gcagacaagg agaagccgat ggccgaggcc ccacaaaatc ccgtacaagt
    10561 gagcgaagca gagcttgagc ggatgcgtca ctcgacggcg cacctcttgg ccgctgccgt
    10621 ttcagcgatc tggccagaag cccgctttgg cgtcggaccg gcgatcaagt tcggtttcta
    10681 ttacgacatt gatgtgccgg tgccgattgg tcccaaagag cttgagcgca tcgagcagaa
    10741 aatgcgcgag ctgcgcaaaa agaagctgcc gtttgagcgc atcgagctgc cgattggcga
    10801 cgcgatttct gaaatgcagc gccgcggcca gcgctacaaa gtcgagcttt tgaccctgct
    10861 gaaagacaaa ggctcgacgg cagttgccaa agaaaccggc gatgaaagcg tcgtcgatgg
    10921 ggccggggca gcgacggtgt cgttttacaa aaccggcgaa tttgtcgacc tgtgccgcgg
    10981 tccgcacgtc tcggtgacga cggaaattgg tgcatttaag ctcaccaatg tggctggagc
    11041 gtactggcgc ggcgacgaaa aaaatccgca gctccagcgc gtctacggtc tggcttttcc
    11101 gacgcaggat gagctggagc ggcacatcca gatgctcgaa gaagccaagc ggcgcgatca
    11161 ccgtcggctc gggcaggaac tggatctgtt cacgttttcg gatctcgtcg gtccgggtct
    11221 gccgctgttt acgcc
//
LOCUS       DAORGN010000165        11224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2445_length_11224_cov_6.198944, whole genome shotgun sequence.
ACCESSION   DAORGN010000165 DAORGN010000000
VERSION     DAORGN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_11224_cov_6.198944"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2960
                     /gene="smc"
                     /locus_tag="PKL17_16060"
     CDS             <1..2960
                     /gene="smc"
                     /locus_tag="PKL17_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNK46300.1"
                     /translation="YRAELRDIELWGMSQRWLGLTLEAKVFFDLVAQSAEALSQVEAA
                     RQTIEAELAAGRLTLVEEETRLQQLQEELYVLDNRVKLSEAENEHARREETRLLEQAR
                     KLRAEQESLYERANQAAQELQKLLEDREALSQEDIGRDDRGASLDSQLKAAKEQLASL
                     QKAADYVKAEVTRQHAEIARSEASERSLLRQVEDIEVRLGRSDEEAKRLRDRKAELDD
                     EIYGQEQKLGGLKQARLDLLKSREEAEERVKDLKLLTSRLDKEVDHLKGELHKKKARL
                     HSLGELAARNEGFSLGSRMLLKRADAKEQKPKTIADLIVAPAELEVALDAVLGERLGA
                     LVLPSHSEAVDAISYLRQQGEGKAFLVPEDARPTRPGERPARPDEEAKSQGIWGRLID
                     LIEKKPGYDGVLDALLGSVLVVDSVEHAVAYYRTASEDGSLPPAACLVTLDGVLIDGH
                     GTMVGGAQKGQGASVLAVRRELRELGTEVADMDERFRTQEEQLVVSKKELSHLQEKLA
                     ELQKAGHQGEMQLLSLDKDLLKGREEHSRVCHRLEVMGREHDELSSRRDEMVREAEEI
                     RLTLRSARELLETLELQLEGHSRGQVGLFDTVEKLGHELTQLKVSQAAHREKVVATER
                     ALSRLREEEVERQSRIERLGGEERDCYKRAAQLATECQSRGSTISEQADRRSVLAEEL
                     QVLRNAYEHKRGALAAREMELRERRDRENKQGKEALAHKARLSELEVQKKALEESVIE
                     RHRVRVEDIISDYHLRPQVSRADEERAAELRELIDRMGEINLTAIDEWNELSKRHGFL
                     VAQKTDLETAIGQLEQAIVLINKTSRQRFQQVFELVNTKFQELFPRCFVGGNAHLKLV
                     GSEDILEAGVEIFAQPPGKKNQTVELLSGGEKAMTAVALIFAIFLIKPSPFCLLDEVD
                     APLDEANVGRFNELIRSMTDRSQFILITHNKRTMQIADTLYGVTMEEPGVSKVVSVNI
                     QEVGRLQGKKRAA"
     gene            complement(2962..4203)
                     /locus_tag="PKL17_16065"
     CDS             complement(2962..4203)
                     /locus_tag="PKL17_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK46301.1"
                     /translation="MQTDQTAPLVWNPFDPEFNRDPYPLLARFRKEQPIYYFEPAKGY
                     IFFRYRDVMNLFREPRLGHDPTLGAGFPPELRAAFPDYVTVRENDLFLVDSESHQRIR
                     KLVNPLFGPRQVESHRARIEQVISGILDGLPSEGVINFFADVAQKFPVRVIASMLNIP
                     EGQERDFLAFADALIATVVPGLPQDVFVGYMPVISRGLQIVRDIVEERRQRPIENDLL
                     SQLISACDEAERLSDAELVSLVAGLLTGGSDTTVHSTTYAMHELLRHPEQLALVRSDA
                     QLARQTLDETLRYNSFGRGAGLPRFAKETFSYEGVTIHRGQPVFLNMMSAWRDPEFVA
                     DADVFDVRRRTHSSPWFGLGPHFCVGASLARLEADMALQMFLSRYPQIELCGEPEYGN
                     HPVFRDIVNLPLRISRTAKAQ"
     gene            complement(4266..5294)
                     /locus_tag="PKL17_16070"
     CDS             complement(4266..5294)
                     /locus_tag="PKL17_16070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46302.1"
                     /translation="MPPPLVSAQQAERKPDRSDAIREYQQIIGQCRDGQAPQAWKAKD
                     DCGLAAFRLGQLYEQLEQFAEAAQAYREVEALSHDPTKVARSLFRAAELFAERLGQIE
                     SAIALCQTIVRTHPAEVAAEDALRAMARWRRGDPTLIVDLDEVAKTQQSHIPLASFAW
                     LLSAQLHEDLGNQTEAVRRYDELAKRYPKGPLLDDALLSAAKTLRSLKRYGEAAERLE
                     RLQRTYTSALIVGHYNLFLLSEGALLLGQIYLDDLKQPARAIPALAGFLKRQPTSRLA
                     DDALVLMAEAARRRHIPPTAADRTESCRYLQQLGEKYPDTNQRRKVDALISQIPCPAA
                     NPGGPARP"
     gene            complement(5348..6394)
                     /locus_tag="PKL17_16075"
     CDS             complement(5348..6394)
                     /locus_tag="PKL17_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNK46303.1"
                     /translation="MLQAVIDLRSDTVTRPTTAMRAAIGAAEVGDDMFGEDPTVRKLE
                     EVVATRLGKAAALFVPSGTMANQLAIRCHVQPGDDVLCSDGAHIKWYEAGAAAALAGA
                     QLVTVGHGGFFTVADLVSTYQPERTDQASPPNRLLCFENTHNRGGGAIWPMQQMYEVA
                     QAARQLGLKIHLDGARLWNAAVALGVPDAEIAAPFDTVSVCLSKGLGAPVGSLVCGPV
                     DVIHKARRFRRMYGGAMRQAGVLAAAGIYALSQNYQRLAEDHHNARRIAEVIGDLRYV
                     SVVPPQTNILLFDLAPPLPDAAEVTARLKAQGVLVAPFGPRRVRLVTHLDVDRTACEE
                     AAQRIRQVLCDALL"
     gene            6440..7423
                     /locus_tag="PKL17_16080"
     CDS             6440..7423
                     /locus_tag="PKL17_16080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNK46304.1"
                     /translation="MVDPRQSLEFERPLVELENKIAELKQLAGGELLAAEIKILERRA
                     SRVRAEIYSNLSPWQKVQLSRHPLRPYTLDYVSKLCSEFFELHGDRAFSDDEAVVGGL
                     ARLTAAADRPVLLIGHQKGRGTKEAMRRNFGMPRPEGYRKGRRLMELAARMKLPIVSL
                     IDTPGAFPGIDAEERGQAEAIAKNLEVMAGLPVPIVSVVIGEGGSGGALALGVCDRLL
                     MLEYSVYSVITPEGCASILWKDQAQVEVAASQLKMTADDLNRLGVTDEIIAEPPGGAH
                     HDLDEAARRVGEAVARHLADLSALSPSQLLDGRYAKYRAIGSFIDTASPPA"
     gene            complement(7420..9024)
                     /locus_tag="PKL17_16085"
     CDS             complement(7420..9024)
                     /locus_tag="PKL17_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46305.1"
                     /translation="MTPEKIARGSTRWVLVPAVLLGLVALLLSVSHRFLPMNDAPSHI
                     ANAVIAHKLLAGDAFFASHYRFDLVPVPYWATALLMLLLQKLFVPLIAWRIITGSYLV
                     LLPLSLLVLWRSMQDPAAQAESQTPADPPPLLPLGVLTVFHWGYWMGESNYLLGQPVA
                     LFALALFCKSRKLGSASFVGFVLLAALSYLCHIYALTLLLIAATAWAFLALCAHKVPQ
                     LSAHKLRLSHVLGLGWIYGLFAVAAYFVLFQHGSDANRGSLGFDLSLRRLVHMLIDPF
                     DSPEPTPRALLLLTYGGLAAVFFFSHRQTLQALWAEKTDRLGRLAQLVEPALLFPGLT
                     VLLAAYLGPVSILAADGSQKEGEIAIRFVLGGFLLSVLSLKLPAGSGKNRPIVAVVAL
                     TTIFAVAQLACAHRLHTAVDDQLQRIDATLLAKIPPHSRVLPLLNKPQAEWRDFLVHR
                     IGNYVVLRDSYSPHIFAVRGQHALRHLPWGDQREVSNLQVTEEEWRYYDYVLLQSLSP
                     PSPQQLAHLTHVATQDDFQLWQVRKN"
     gene            complement(9041..10027)
                     /locus_tag="PKL17_16090"
     CDS             complement(9041..10027)
                     /locus_tag="PKL17_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46306.1"
                     /translation="MSAKVQAAPTWRLLLNNLFVLRFNPVGLEDQLRLGIQRRLPAIE
                     GSEGLLFRDRFVFAGIAPRLNPAFVKIGPSLELQPLSILNLRVTAEYMYLFSTFGFLQ
                     SFASARDDYSDPLMAACASADPTTRESCRYTDGQGVVQQGSAERRNYRTGGLHMMVEP
                     TVQLKLGPVLLRNRLSLEYWYMDLRDGDRVFFDVTLDTLVPARGWVLANDLDLAWMFK
                     FGLMAGVRYSVVQPFYSGNDLRPGEFVDFVHNGVQRIGPILSYTFFDRGFVRFNKPTI
                     LLISGFYVSHRYRAGQEPAAVFPGLIVQNPAMPYLVIGFSFQSDLLSPAAKR"
     gene            10388..>11224
                     /locus_tag="PKL17_16095"
     CDS             10388..>11224
                     /locus_tag="PKL17_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46307.1"
                     /translation="MTAERVRKCPECGRTYPIKVLYCRTDGCQLGDPGEELASAVKPT
                     PGRDQLLGRTVARRFVLTEYIGSGATGLVYRAVHKALDREMAVKLLKRELLWDERSLL
                     RFFREAKTCSAVDHPNIVYLYDFGHDDTTGLPYLVMEFVSGQTLHQAIHGSPTGNLPV
                     ERALAILLQVCHAIEHAHNRGVIHRDIKPENILLTERDGRADWVKVLDFGVARMVGEP
                     PVTGHGQISGTAEFIAPELLIGDGSVTPAGDLYALGILFHDAIVGRPPFSGRLEIVLQ
                     QHM"
BASE COUNT         2375 a         3470 c         3446 g         1933 t
ORIGIN      
        1 gctaccgggc cgagctgcgc gacatcgagc tgtggggcat gagccagcgc tggcttgggc
       61 ttacgctgga agcaaaagtg tttttcgact tggtggcgca gtcggcagaa gcgctttcgc
      121 aggtcgaagc ggcgcgtcag accatcgaag cagagcttgc cgccggacgg ctcaccttgg
      181 tggaagaaga gacgcggctt cagcagctcc aagaggaact ctacgtactc gacaaccgcg
      241 taaaactttc ggaagcggaa aacgagcacg cgcgacgcga agagacccgg cttctggagc
      301 aagcacgcaa gctgcgggcc gagcaagagt cgctgtacga gcgcgcaaac caagccgcgc
      361 aggaactgca aaagcttctc gaagaccgcg aagcgctgag ccaagaagac atcgggcgtg
      421 acgatcgcgg agcctcgcta gacagtcagc tcaaagccgc caaggaacag cttgcgtcgc
      481 tacaaaaagc cgccgattac gtgaaggccg aagtcacccg ccagcacgcc gaaattgcgc
      541 gctcggaagc gagcgaacgg agtcttctgc gccaagtcga agacatcgaa gtgcggctcg
      601 gacgcagcga tgaagaagcc aagaggctgc gcgaccgcaa agccgagctg gacgatgaaa
      661 tctatggtca ggaacagaag ctcggcgggc tcaagcaggc ccggctcgat ctgttaaaga
      721 gccgcgaaga agccgaggag cgcgtcaaag acctcaagct cttgacctcg cggctcgaca
      781 aagaagtcga ccatctcaaa ggcgagctac acaaaaagaa agcccgtctt cactcgcttg
      841 gcgagctggc ggcgcgcaac gaaggttttt cgctcggcag tcgcatgctg ctaaagcgtg
      901 ccgatgccaa agagcagaag cccaagacga ttgccgacct gattgtcgcg ccggcagagc
      961 ttgaagtcgc gctggatgcg gtgctggggg aacgcctggg ggcgctggtc ttgccgtccc
     1021 acagcgaggc cgtcgacgcc atttcgtatc tgcgccagca gggtgaaggc aaagccttcc
     1081 tggtgcccga agatgcgcgg cccacccgtc caggcgaacg tccagcccgt cccgatgagg
     1141 aagccaagag ccaaggcatc tggggcaggc tgatcgatct gattgaaaaa aagcccggct
     1201 atgacggtgt gctcgacgcg ctgctcggat ctgtgctggt ggtcgattcg gtagagcatg
     1261 cggtcgcgta ctaccgcacc gccagcgaag atggcagcct gccgcccgcc gcgtgcctgg
     1321 tcacgctgga tggcgtgctg atcgatggcc acggcacaat ggtcggtggc gcgcagaaag
     1381 ggcagggcgc gtcggtcctg gctgtccgtc gcgagctgcg cgagctgggc actgaagtgg
     1441 cggacatgga cgagcgcttc cgaacccagg aagaacagct cgtcgtcagc aaaaaagagc
     1501 tgtcccacct gcaagaaaag ctcgccgagc tgcaaaaagc cgggcatcag ggcgaaatgc
     1561 agcttctgtc gctcgacaaa gaccttctca aagggcgcga agagcacagc cgggtctgtc
     1621 atcggctgga agtcatgggt cgcgagcacg acgagctttc gtcccgtcgc gatgaaatgg
     1681 tccgcgaggc cgaggaaatc cggctcacgt tgcggtccgc gcgtgagctt ttggaaaccc
     1741 tcgaactgca gctcgaaggg cacagccgcg gccaggtcgg tctgttcgat accgtcgaaa
     1801 agcttgggca cgaactgacg cagctaaaag tgtcccaggc ggcccatcgc gaaaaagtcg
     1861 tcgccaccga gcgggcgctc agccgcctgc gcgaagaaga ggtcgaacgg cagtctcgca
     1921 tcgagcggct cggcggcgaa gaacgcgact gctataagcg cgccgcgcag cttgccaccg
     1981 aatgccaaag ccgcggcagc accatcagcg aacaagccga caggcgcagc gtgctcgctg
     2041 aagaactgca agtgctgcgc aatgcctacg agcacaagcg gggcgcgctg gccgcacgcg
     2101 aaatggagct gcgcgagcgc cgtgatcgcg aaaacaagca gggcaaagaa gcgctcgccc
     2161 acaaagcccg cttgtccgag ctggaagtgc agaaaaaggc gctcgaagag tcggtcatcg
     2221 aacgccaccg cgtccgcgtc gaagacatca tcagcgacta ccatctgcgc ccacaagtca
     2281 gccgcgccga cgaagaacgc gccgccgagc tgcgggaact tatcgaccgc atgggcgaaa
     2341 tcaacctgac cgcgatcgac gaatggaacg agctgtccaa gcgccacggg ttcctggtgg
     2401 cgcaaaagac cgacttggaa accgcgattg gtcagctcga acaggcgatt gttctgatca
     2461 acaaaaccag tcggcagcgc ttccagcagg tcttcgagct ggtcaacacc aagttccaag
     2521 agcttttccc gcgctgtttt gtcggtggca atgcccacct aaagcttgtc ggcagcgaag
     2581 acatactcga agccggcgtc gaaatttttg cgcagccacc aggaaaaaag aaccagaccg
     2641 tcgagctgct ctccggtggc gaaaaagcga tgactgcggt ggcgctgatt ttcgccatct
     2701 tccttatcaa gccatcgccg ttctgtctgc tggatgaagt cgatgcaccg cttgacgaag
     2761 ccaacgtcgg caggttcaac gaactgattc gcagcatgac cgatcgcagt cagttcatcc
     2821 tcatcaccca caacaagcgg acgatgcaga ttgccgacac cctgtatggc gtgacgatgg
     2881 aagaacctgg cgtctcgaaa gtcgtgtccg tcaacatcca ggaagtggga cggctgcaag
     2941 gcaaaaagcg cgcggcttag ctcactgcgc tttggcggtg cggctgattc gcaggggcag
     3001 gttcacgatg tcgcggaaca ctggatggtt tccgtactcc ggctcaccgc agagctcgat
     3061 ctgtggatag cgcgacagaa acatttgcag cgccatgtcc gcttcgagcc gcgccagcga
     3121 agcgcccacg cagaagtggg gccccaggcc aaaccacggg cttgagtgcg tccgccgccg
     3181 gacatcgaac acgtcggcat cggcgacaaa ttcaggatca cgccacgccg acatcatgtt
     3241 caaaaacacc ggctggccgc ggtgaatcgt gacgccttcg tagctgaacg tctcttttgc
     3301 aaagcgcggc aggccggcgc cccgaccaaa tgagttatag cgcagcgttt cgtccagcgt
     3361 ttgccgggca agctgcgcgt cgctgcgcac cagcgccagt tgttcgggat gacgcagaag
     3421 ctcgtgcatc gcatacgtcg tcgagtgcac cgtcgtgtcc gacccacctg tcaaaagacc
     3481 cgcaaccagc gagacaagct ccgcatcgct cagccgctcg gcctcatcgc atgccgaaat
     3541 gagctggctt agcaggtcgt tttcaatcgg tcgctgccgc cgttcttcga cgatatcccg
     3601 cacgatctgt agaccgcgcg agatcaccgg catgtagccc acgaacacgt cctgcggcag
     3661 ccccggaacc accgtcgcga tcagtgcatc ggcgaacgcc aaaaagtcgc gctcttgccc
     3721 ttcgggaatg ttcagcatcg acgcaatcac gcgcaccggg aacttctgcg cgacatcggc
     3781 aaaaaagttg atcacgccct cgctgggtag cccatcgagg atgcccgaaa tgacctgctc
     3841 gatccgggcg cggtggcttt ccacctgacg cggcccaaac agtggattta ccagcttgcg
     3901 aatccgctga tgcgattccg aatcgaccaa aaacaggtca ttttcccgaa ccgtcacgta
     3961 atccggaaac gccgcgcgca gctccggcgg aaatccggca cccagcgtcg ggtcgtgccc
     4021 aagccgcggc tcgcgaaaca ggttcatcac gtcgcggtag cggaaaaaga tgtagccctt
     4081 cgccggctcg aaataataaa tcggctgctc cttgcggaag cgggccagca gcggatacgg
     4141 gtcgcggtta aattccgggt cgaagggatt ccataccaaa ggggcggttt gatcggtttg
     4201 catggccgga cagcatagca cgtcacccag caccaccgac gggacgcgct aagtccgcag
     4261 gaagactagg gccttgccgg gccaccagga tttgccgcag ggcaggggat ctgcgaaatc
     4321 agcgcatcga ctttgcggcg ctggtttgtg tccgggtact tttcgcccag ctgttgcaga
     4381 tagcggcagg actctgtgcg atcggctgcc gtcggtggaa tgtgtcgtcg tcgcgccgct
     4441 tcagccatca gaaccagtgc atcgtccgcc aggcgcgaag tcggctgccg tttcaaaaaa
     4501 cccgccagtg cgggaatcgc gcgcgccggc tgcttcagat catccaggta gatctgcccg
     4561 agcagaagcg cgccttctga aagcaaaaac aggttgtagt ggccgacgat cagcgccgac
     4621 gtatacgttc tttgcagtcg ctccagccgc tctgccgctt cgccgtagcg cttgaggctt
     4681 cgcagggtct tggccgccga caaaagcgca tcatcgagca gcgggccttt cgggtagcgc
     4741 ttcgccagtt catcgtacct tcgcaccgct tcggtctggt tgcccaagtc ttcgtgaagc
     4801 tgcgccgaaa gcagccaggc aaagctcgcc agcgggatgt ggctctgctg cgtctttgcc
     4861 acttcgtcta aatcgacaat cagcgtcgga tcgccgcgcc gccagcgcgc catcgcccgc
     4921 agcgcatctt ccgccgccac ttccgcagga tgcgtgcgca caatcgtctg gcacagcgca
     4981 atcgctgact cgatctgtcc cagtcgttcg gcaaacagct ctgccgcccg aaacagcgag
     5041 cgcgccacct tggtcggatc gtgactcagc gcttcgactt cgcgatacgc ctgtgcggct
     5101 tccgcaaact gttctagctg ttcgtaaagc tgcccgagcc gaaaggccgc aagcccgcag
     5161 tcatctttcg ccttccacgc ctgcggggct tggccgtcgc gacattgtcc gatgatctgc
     5221 tggtattcgc ggatcgcatc gctgcgatcg ggctttcgct cggcctgctg cgccgacaca
     5281 aggggcggcg gcatcggtgc aggctttgcg cacccgccac aaagcgccac cagccaaagc
     5341 ccaagcctta cagcagcgcg tcgcataaca cctgccgaat ccgctgcgcc gcttcctcgc
     5401 aggccgtgcg atcgacatcc aggtgcgtca ccagtcgcac ccgccgcggg ccaaacggtg
     5461 ccaccagtac cccttgtgct ttgagccgcg ccgtcacttc ggcggcgtcg ggaagcgggg
     5521 gcgcaaggtc aaatagcagg atgttcgtct gcggcggcac aaccgacacg tagcgcagat
     5581 ccccgatgac ctcggcaatc cgtcgcgcat tgtggtgatc ttccgcaagc cgctggtagt
     5641 tttgcgacag cgcgtatatc ccggctgcgg ccagcacacc cgcctgccgc atcgcgccgc
     5701 catacatccg gcgaaagcgc cgcgctttgt gaatcacatc gacgggacca cagaccagcg
     5761 aaccgacggg cgcacccagt cctttggaca ggcagaccga caccgtgtca aacggcgcgg
     5821 caatttccgc gtcgggcact cccagcgcca ctgccgcatt ccacagccgc gcaccatcca
     5881 ggtggatttt caggccaagc tgccgtgctg cttgcgccac ttcgtacatc tgctgcatcg
     5941 gccagattgc gccaccgcca cggttgtggg tgttttcgaa gcacaaaaga cgattgggcg
     6001 gcgaggcttg atccgtgcgc tctggctgat aggtcgaaac caagtcagcg actgtgaaga
     6061 aaccaccgtg ccccaccgtg acaagctgcg ccccggccag cgctgctgcg gccccggctt
     6121 cgtaccactt gatgtgggca ccgtccgaac acagcacgtc gtcgcccggc tgcacatggc
     6181 agcgaatcgc aagctggttt gccattgttc ccgatggcac aaacagcgcc gccgctttgc
     6241 ccagccgggt cgccaccact tcttcgagct tccgcaccgt ggggtcttcc ccaaacatgt
     6301 catcgccgac ttccgccgcg ccaatcgcag cccgcatggc tgtcgtcggg cgggtcaccg
     6361 tgtccgagcg aagatcgatg acggcttgca acatgcggcg agcataccca aaaatagcgc
     6421 cacttcgtaa aggcttctca tggtcgatcc acgtcagtcg cttgagtttg aacgtccgct
     6481 ggtcgagctg gaaaacaaga tcgccgagct aaaacagctg gccggcggcg aactgctggc
     6541 cgccgagatc aagatcctcg aaagacgagc cagccgggtt cgtgccgaga tttattccaa
     6601 cctgtcgccg tggcagaaag tgcagctctc gcgccatccg ctgcggccct acaccctcga
     6661 ttacgtgtcc aagctgtgca gcgagttttt cgagctgcat ggcgatcgcg cgttttccga
     6721 tgacgaagcg gtggtcggtg gtttggctcg tctgacagcc gcggcagacc ggcccgtgct
     6781 cttgattggg caccaaaaag gtcgtggcac caaagaagcg atgcggcgca atttcggcat
     6841 gccgcgtccc gaaggctatc gcaaaggccg tcgtctgatg gaactggcgg cccggatgaa
     6901 gctgccgatt gtgtcgctta tcgacacgcc cggcgccttt cccggcattg atgccgaaga
     6961 acgcggccaa gccgaagcga ttgccaaaaa cctggaagtg atggccggcc tgccggtgcc
     7021 gattgtctcg gtggtcattg gcgaaggcgg atcgggcggg gcgctcgcgc ttggggtctg
     7081 cgatcggctg ctgatgctgg agtattcggt ctattcggtc attacccctg aaggctgcgc
     7141 gtcgatcctg tggaaagacc aagcccaggt cgaagtcgcc gcctcgcagc tcaagatgac
     7201 cgcggacgat ttgaatcggc tgggcgtgac cgacgagatc atcgccgaac cgcctggcgg
     7261 tgcccatcat gacctcgacg aagcggcgcg gcgcgtcggt gaagccgtgg ctcgccacct
     7321 ggctgatctg tcagcccttt cgccgtcgca gcttctcgat ggccgctacg ccaaataccg
     7381 tgcgatcggg tcgtttatcg ataccgcgtc gccgcccgct tagttttttc gcacctgcca
     7441 aagctggaag tcgtcttgcg tcgccacatg cgtcaagtgg gcaagctgct gtggcgatgg
     7501 cggggaaagc gactgaagca gcacatagtc gtagtagcgc cactcctctt ccgtcacttg
     7561 caggttcgac acctcgcgct ggtcacccca cggcaggtgg cgcagcgcat gctgcccgcg
     7621 gactgcgaag atgtgcgggc tgtacgagtc gcgcagcacc acgtagttgc cgatgcgatg
     7681 gaccaggaag tcgcgccact ctgcctgcgg tttgtttaga aggggcagca cccgactgtg
     7741 cggtgggatc ttggccagca gcgtcgcgtc gatccgctga agctggtcat cgaccgccgt
     7801 gtgcagccga tgggcgcagg caagctgcgc gacggcaaag attgtcgtca gcgcgacgac
     7861 ggccacaatg ggtcggtttt tgccgctgcc tgctggaagc ttcagcgaca gcaccgacag
     7921 caaaaaaccg cccagcacga agcgaatcgc aatctcgcct tctttctggc tgccgtcggc
     7981 agcgaggatg ctcaccggtc ccaaataggc cgccaaaagc accgtaagac ccggaaacag
     8041 aagcgccggc tcgacaagct gggcaagccg cccaaggcga tccgtttttt ctgcccaaag
     8101 cgcttgcagc gtctgccgat gcgaaaagaa aaacaccgca gccaggccac cgtaggtcag
     8161 caaaagcagc gcccgtggcg tcggctcggg cgaatcaaac gggtcaatca gcatgtgcac
     8221 aagccgccgc agcgacagat caaagccgag cgatccgcgg ttggcatccg atccatgctg
     8281 gaacaggaca aagtacgctg cgacggcaaa cagtccgtag atccagccga gtcccaagac
     8341 gtgcgaaagg cgcagcttgt gggccgaaag ctgcggcact ttgtgggcgc acagggccaa
     8401 aaacgcccac gccgtcgctg caatcaaaag cagcgtcagc gcgtagatgt gacacagata
     8461 ggacagcgca gcgagcagca cgaagccgac aaacgacgcc gatcccagct tgcggctttt
     8521 gcaaaacagg gccagcgcaa acagcgccac cggctggccg agcaggtagt tggactcgcc
     8581 catccagtaa ccccagtgaa acaccgtcag cacgccgagc ggcagaagcg gtggcggatc
     8641 ggctggggtt tggctttccg cctgcgccgc cgggtcttgc atcgatcgcc acagcacgag
     8701 cagcgacagc ggcaaaagca caaggtagct gcccgtaatg atccgccagg cgataagtgg
     8761 cacaaagagc ttttgcaaaa gcagcatcag gagcgccgtc gcccaatacg gcaccggcac
     8821 aaggtcgaag cggtaatgac tggcaaagaa ggcgtcccct gcaagcagct tgtgggcaat
     8881 caccgcgttg gcgatgtggc tcggcgcgtc gttcatcggc aagaagcgat gactgaccga
     8941 cagcaacagt gcgacgagcc caagcagaac tgcggggacc aaaacccagc gcgtgctgcc
     9001 gcgagcgatc ttttccggcg tcatggcctc tgggaccgcg ctaccgcttc gccgctgggc
     9061 ttagcaagtc cgactggaac gaaaagccaa tcaccaggta cggcatcgcg ggattttgaa
     9121 cgatcagacc cggaaaaaca gcggcgggtt cctgaccggc gcggtagcgg tgcgatacgt
     9181 agaatccgct gatgagcagg atcgttggct tgttaaagcg cacaaagccg cgatcaaaga
     9241 aggtgtacga caggatcggt ccgatgcgct gcacaccatt gtggacaaag tcgacaaact
     9301 cgccggggcg taggtcgttg ccgctgtaga acggctgcac cacgctgtac cgaacgccgg
     9361 ccatcaggcc aaacttgaac atccaagcaa ggtcaaggtc gttggccaga acccagccgc
     9421 gagccggcac cagcgtatcg agtgtcacgt cgaagaagac gcgatctcca tctcgcagat
     9481 ccatgtacca gtactcaagg ctcagtcggt tgcgaagaag caccgggcca agcttgagct
     9541 gaaccgtggg ctcgaccatc atgtgaagac cgccggtccg gtagttgcga cgttctgcgc
     9601 tgccttgctg caccacgcct tgtccatcgg tataacggca gctttcgcgg gtcgtggggt
     9661 ccgcgctcgc acaggccgcc atcagcgggt cggagtagtc gtcccgtgcc gaggcaaacg
     9721 actgcaagaa gccgaacgtc gagaacaggt acatatactc ggcggtgacg cgcaggttca
     9781 ggatcgacag cggctggagt tctagcgacg gtccaatctt cacaaacgct gggtttaggc
     9841 gcggtgcaat gccggcaaat acgaagcgat cgcggaacag cagaccttca cttccctcga
     9901 tcgcggggag ccggcgctga atgccgaggc ggagctggtc ttcgagtcca accggattga
     9961 accgcagtac aaacaggttg tttagcagca gtcgccacgt cggtgcggcc tgtaccttgg
    10021 ccgacatggg atcagtctgt ggcggtggcc ccaaacccgt ttcttgcggc accggcgtcc
    10081 tgggctcgct cgcaatcggc gacggggccg gttgcgcatt cgccgctggc tccgctagag
    10141 caaacggatc tctgatctgg gcggtcgcat cgtggctgag tcccacagcc caaaggcaaa
    10201 caacgaaacc aagcatgcga gtcgagaaca tcggcggact ataggtgcct ttgcttggac
    10261 cgcaaaacta caaaagcttt tggaaaggcg gaccgatttg tcacaactcg aggcaagatg
    10321 aggggctaag ctcgctgccc ttgccaaagg ggatggaccc agagggtagg ggtggggcaa
    10381 acggacgatg actgccgagc gtgttcgcaa gtgtcccgag tgtggccgga cgtatccgat
    10441 caaggtgctg tattgccgca ccgatggatg ccagcttggc gatccgggtg aggaactcgc
    10501 ctcggcggtc aagccgacgc cgggacggga tcagctgctg ggccggaccg tggcgcgacg
    10561 ctttgtgctg accgaataca ttggctcggg ggccacaggt cttgtctacc gcgcggtcca
    10621 caaagccctc gatcgcgaga tggcggtcaa gctgctcaag cgcgaacttt tgtgggatga
    10681 gcgctcgctt ttgcgctttt tccgtgaggc caagacctgt agcgccgtcg accaccccaa
    10741 catcgtgtat ctgtacgact ttggccatga cgacaccacc gggctgccct acctggtcat
    10801 ggagtttgtt tccggacaga cgctgcacca agccatccac ggcagtccca ctggcaacct
    10861 acccgtcgaa cgtgcgctcg ctatcttgct tcaggtctgc cacgccatcg aacacgccca
    10921 caaccgcggc gtgatacacc gagacataaa accggaaaac atcctgctta ccgagagaga
    10981 tgggcgggct gactgggtca aggtgctcga ctttggcgtc gcgcgcatgg tcggtgaacc
    11041 gccggtgacg ggtcatggtc agatttccgg caccgcggag ttcattgccc ccgagctgct
    11101 gattggtgac ggcagcgtga ccccggcggg cgatctgtat gcgcttggca tcttgtttca
    11161 tgacgccatc gtcggacgtc cgccgttttc gggtcggctg gagatcgtgc ttcagcagca
    11221 catg
//
LOCUS       DAORGN010000166        11132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2470_length_11132_cov_5.726551, whole genome shotgun sequence.
ACCESSION   DAORGN010000166 DAORGN010000000
VERSION     DAORGN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_11132_cov_5.726551"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3604
                     /locus_tag="PKL17_16100"
     CDS             <1..3604
                     /locus_tag="PKL17_16100"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNK46308.1"
                     /translation="GLPSTWDVGNKPPGDLLDRGTTGFVDVPLSSGDRPPPIGILEFA
                     PPAQHAIPEFASRCQVAATVSDDAFQRSGAQTEYIVHPARYYLATQTPVKQARSGDVI
                     KLPVRALFYDGSRLSAPGVRVQIERRAIVPVYRSEHGIEVLVRHEQKLEPIHSCTLDI
                     PNTGTDPECVFAANAVGDYDVTLRGATSADTRGAVTRTSFSVLPKENAVAVVAQKTVV
                     DRLYIETGQETVKHHSTLHVSVRGPRGVERGLLLSERNGLREHVVLHFRDGVAHHEFA
                     TDDSWVAGVKLRALAVYASPGSQPTVLEAQATVRGEIDALRLRVQIEVPKEAGPRAQV
                     PIAVKVTDRATGVPIAGARVSLWAVDEAAMDFTQSMVTPDRLSQSLVSHFLPHHRIET
                     SRRDAFSTILRPYVRQPKEPWLPARIESEDATTTPGNTGKVSKESVDAKPVALDARND
                     IMATPLFIADLPTGPDGKLITTLPLPDSLTTFRIFAVVTAPMSDKSSPGCFGASDARV
                     RVSAPMVLRAAIPRLLRPGDRAEVTALVQNHTNMRGNLTVQARLKFDGLLPPLTLLAP
                     PQIEQSMDRNVPAKIPIEVQGNHAGTAEIELSASFVPTSRTAKKDVFTDVVRLPIVVE
                     QERTQVERAVAFGVLHDAQAILIPVQLPANALPAPGGISVSMSSSGLVQIEDAARQLL
                     ASPHRSLEQGASRLVPPVIIPELFRGTGSGLPDVKSAVQQSVQRLLSLQLPHGGFAFW
                     PDDQRAHPFGSAYATWLLHKAAQAGHPVPSDALQRATTYLQRLLASPQPGTEGQLLEP
                     VTVAQAIMLARKEAEQPGSTVTPLSFDYFDNTQRALVAFVLAEMGQANPAANGVLFAN
                     RAMLPLFARALLLLTLHKTAPEDLRIATLTEEVLSTVSASERSAHVREQLPYNLDALF
                     HSPVRTQAAVLMALLRVQPKNANLLKLARGLLFERVQGAWKSTQDNAWAILALLDYNR
                     LHPVEGSDISAGAWLLDEQLMSAKMPSPQSPPQRVDFTLQKMLQLVPPDEGKQSLIEL
                     PLTLQRSGRGTMHYRVGLEWTTAQTGMGATSQGIKFRRALRTAAGALPSTGSLLAGEA
                     VAMDIDLSCQQPVHHLVLDIPLPAGLEAIQREGAATSSAALAGPRSGQISFEEIRRDR
                     VLIYFDALPAGDHEHTLQLRSTTAGRYMVPPAKATALYDPGLQGSTDAVNIWVH"
     gene            complement(3639..5378)
                     /locus_tag="PKL17_16105"
     CDS             complement(3639..5378)
                     /locus_tag="PKL17_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46309.1"
                     /translation="MKKSWKRFLPMLLAGISGFVFSVCDSGGTSGQCTGGTDCATPSC
                     TDKVQNGGETDIDCGGSCSTKCGAQAKSQNGTDCQSGLVCPAGVCIAPTCTDGILDGS
                     ETDKDCGGACMKCAPNAACKVAADCTSANCASGVCAAPSCTDGIKNGDESDVDCGGSC
                     NACAVGKTCVADKDCGIFTCGTPVPEGQTPVCGLGNGADGDLAIPSGMTRTINTAATS
                     GTGTMGGTTMTVRDGSPFLVDQVVLIHQTQGTGAGQHEMNRIATVSGTQLTFKNPLKY
                     DYVTGAQVVAVPQYNSVNVTAGGFLTAPAWDGQKGGILVFLSRGDVAAGGTITMASKG
                     FRGAGVTGVCFPSAPGCTLNHGRTGESSLGASAFSTLNGQAQGMNNGGGGGGGSRGQD
                     CAAGGGGSYGTAGTNGVDGTIGACIVVGGQHRGGLAGTAVGGQDLFQSIFLGSAGGEG
                     GPDEDGAYPGPGGNGGGIVMWNSKSVTLDGTASITVSGANGGNGSNSGPCGGGGRGMG
                     NGGGGSGGAVRIQTADSATLNTGKIVASAGAGGNAGSCGATYPGGNGGLGRIHIRALG
                     TLSGTTTPPAFTN"
     gene            5558..6178
                     /locus_tag="PKL17_16110"
     CDS             5558..6178
                     /locus_tag="PKL17_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018881297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNK46310.1"
                     /translation="MRGMSLLVLASASPRRMDMLAAAAVPHAVWPVDIDETPQPGETP
                     AALVERLAEEKAVAASALCPSGSPILAADTIVTVDGALLGKPPHEGAARQMLRLLSGK
                     THEVLTGYHLRYVNRQGELQKLRRVVTTQVTVRELTDGDIAGYLRSEEWRGKAGSYAI
                     QGRFAAFVCALSGSYENVVGLPLCSVLVDLRAADLLPADWPSWTSG"
     gene            complement(6195..7286)
                     /locus_tag="PKL17_16115"
     CDS             complement(6195..7286)
                     /locus_tag="PKL17_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNK46311.1"
                     /translation="MKERSVLVSGAFIVANLLSISAAQAQHHGPFAIRAGQKCLDLNG
                     PQVKVNGGRVQLWDCTGAANQLWRWDRGRIVSMANNRCLDLNGPDTGDNGGRIQVWDC
                     HGGPNQHWRHERGALIVQADNRCLDVHGPDIGQNRAHVQSWDCHFGPNQQWIIEPVAP
                     QAPPAQAPLTCESRLGPFAWSCARPIPGMICEQIREPADPHTWSDNFFCSDRPLGMKW
                     SNSGLIPGMRCTQIVEPADPHTWNDNFLCLPHGSPYRLQWSYSGPIPGLACVAWNEPS
                     DPHTWHDNFLCFSERQEPGPVPMPPPGPMPPPPPPPAPVVREVEAGPIWNNDHAAQRC
                     PAVCQPGRWTGSWRTTIPGRMSICSCAPY"
     gene            complement(7497..9419)
                     /gene="htpG"
                     /locus_tag="PKL17_16120"
     CDS             complement(7497..9419)
                     /gene="htpG"
                     /locus_tag="PKL17_16120"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNK46312.1"
                     /translation="MTSSQTEPKATETRTFSTEVQQLLHLMVHSLYSKKEIFLRELVS
                     NASDAIDTLRFLALTRPELSDAVSAQESAIEIAVQKDDKTLTISDTGIGMTRQQVIDN
                     IGTIARSGTGALIKRLKEQGTDAAKSLDVIGQFGVGFYSVLMVAHKVEVDTRSAEPDS
                     QPILFRCDGGGTYELGEGTRTTPGTTVKLFLNDDASEYLESWQIEHIIKQYSNYVKWP
                     VRLDGKQLNRATALWSKRPSEVKEEEYSDFYKELCGGFSADDVPFGKLHLSIDTPYQF
                     QAIVFIPKKPPFDMMMDQNRRRGMQLYVRRVFILDHAEELLPPWLRFVRGVVDSDDLP
                     LNVSREILQKNQIITTIQKQIVKKVLEELKRLREQKPEDYRAFFEEFGGIIKEGIFLD
                     HANKEKVAEVCLWSSLNTEAAERITIADYIAKMPAGQDEIYYITGPSRTVVERSPHVE
                     ALRKRKLDVLLFIDPIDEWVVQSLSEYSGKKLRAIHKGDFTPPKIEGESEPEADKQTP
                     EQQTELQGLLAHLRVRFQERVKEVRLSSRLTDSASVLVAEEGDLGAHLEQLLQRAGRK
                     VEVKKPILELNPQSPIVEKLAHLLAVRPGSEEINTYSDLLLELAYLAQGSVPEPGALL
                     SSLNRVLSRDLSSLAG"
     gene            9519..10019
                     /locus_tag="PKL17_16125"
     CDS             9519..10019
                     /locus_tag="PKL17_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46313.1"
                     /translation="MESNTPSDRDTEDSPSGAGPSDPLLSLQIVWVALLVSLGIYAVI
                     AYTVAKQVANGAQLAPLPAMQMAFAMISLSSVTVAYVLSRKLLKQAIGTDDPATLPVA
                     KLARLAFTPWILRMAMTESVGIFGLLMAMLTGQPSSGLPFLIVSALVMLTHLPNEKAL
                     RTAAQQ"
     gene            10261..10836
                     /locus_tag="PKL17_16130"
     CDS             10261..10836
                     /locus_tag="PKL17_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46314.1"
                     /translation="MRDKKHTTGMLGMALAAALSWSFPARAETPAVPDKLGRHMVNAR
                     VAAELLTGWDSGMGWTVPVVAIEPAIGVTSSRNGYVVFPFSVQNFFNATALGIGIGYQ
                     HDIPLPIRRLYATARATVSYESYQIRNGSLFNGSITHSHTAAFQPSIGVKYVLAGRVN
                     LGIDFASIKLRIQNGLYDASYQFVGSAGVTL"
     gene            complement(10826..>11132)
                     /locus_tag="PKL17_16135"
     CDS             complement(10826..>11132)
                     /locus_tag="PKL17_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46315.1"
                     /translation="GKKSQKVWHQEFFVDAVRHGVFIGGSNRLGQEPPWTIEYFGESY
                     FCGPQGILPEISAGPGLVMADVDLSELSEPDPSGWNLPRDIRYDIYSPRPQRRNVTA"
BASE COUNT         2087 a         3293 c         3449 g         2303 t
ORIGIN      
        1 gggcttgccg tcgacctggg atgttggaaa caagccgccg ggcgatctgc tggaccgcgg
       61 aacgaccgga tttgtcgatg tgccgctgtc gagtggcgac cggcccccgc cgattggcat
      121 tttggagttt gcgccaccag cccagcacgc gattccggag tttgccagcc gttgccaggt
      181 tgcggcgacg gtttccgacg atgcctttca gcgttccggc gcgcagaccg agtacatcgt
      241 gcatccggcg cggtattact tggcgacgca gacgccggtc aaacaagctc gcagcggcga
      301 cgtcatcaag ctgccggtgc gggcgctttt ctacgatggt tcgcgactgt ccgcgccggg
      361 ggttcgggtg cagatcgagc ggcgcgcgat tgtgccggtc tatcgcagcg aacacggcat
      421 cgaggtcttg gtccggcacg agcagaagct tgagccgatc cactcctgta cgctggacat
      481 tcccaatacg gggacagatc cagagtgtgt ttttgccgcg aacgccgtcg gtgattatga
      541 tgtaacactg cgcggcgcaa ccagtgcgga tacccgtggg gcggtgacgc gaacgtcgtt
      601 ttccgtgctg ccaaaagaaa acgcggtagc agtggttgcg caaaaaacgg tggtcgatcg
      661 gctctacatt gagacgggac aagagacggt caagcaccac tcgacgctgc acgtctcggt
      721 ccggggtccg cgcggtgtcg aacgcggcct gcttttgtcg gaacgcaacg gcctgcgtga
      781 acatgtggtg ttgcacttcc gtgacggcgt ggcccatcac gagtttgcga ctgatgactc
      841 gtgggtggcg ggagtgaagc tgcgggcgct cgcggtgtac gcaagtccgg gctcgcagcc
      901 gacggtgctc gaagcgcaag cgacggtgcg cggggaaatc gatgcgctgc gactgcgggt
      961 gcagattgaa gtgcccaaag aagccggtcc gcgggcgcaa gtgcccattg ccgtcaaagt
     1021 gacggatcgg gcgacgggtg ttccgattgc cggggcgcgg gtttcgctgt gggcggtcga
     1081 cgaagcggcg atggatttta cccagtcaat ggtgacgccc gatcggctgt cccagagcct
     1141 ggtgtcgcac tttttgccgc accaccgaat tgaaacgagc cgacgcgatg cgttttcgac
     1201 gattttgcga ccgtatgtgc gtcagccaaa agagccctgg ctgccggcgc ggatcgaaag
     1261 cgaagatgcg acgacgacgc cgggaaacac cggcaaagtg tcaaaagaaa gtgtggatgc
     1321 caagccggtc gcactcgatg cgcgcaacga catcatggcg acaccgctgt ttatcgccga
     1381 tttgccgacg ggcccggacg ggaagctgat cacgacgctg cctctgcccg atagcctgac
     1441 gacgttccga atttttgcgg tggtgaccgc gccgatgtcg gacaaatcga gtccgggctg
     1501 tttcggtgcg tcggatgcgc gcgtgcgggt gtcggcgccc atggtgctgc gcgcggcgat
     1561 tccgcggctg ttgcgaccgg gcgatcgggc ggaagtgaca gcgctggtac aaaaccacac
     1621 caacatgcgc ggcaacttga cggtgcaggc caggctcaag ttcgatgggc ttttgccgcc
     1681 gctgacgctg ctggctccgc cgcaaattga gcagtcgatg gaccgcaacg tgccggccaa
     1741 aattcccatc gaagtgcaag gcaaccacgc tggcaccgct gaaatcgagc tgtcggcatc
     1801 gtttgtcccg accagccgga cggcaaaaaa agatgtcttc acggatgtgg tccgcctgcc
     1861 aattgtcgtc gagcaagagc gaacccaagt cgaacgcgcg gtggcgtttg gcgtgctaca
     1921 tgacgcgcag gcgattttga ttccagtgca gctgccggca aatgcgctgc ccgctccagg
     1981 cggtatttcg gtgtcgatgt catcgtcggg gcttgtgcaa atcgaggatg cggcgcggca
     2041 gcttttggct tcgccacatc gatcgctgga acaaggtgcg tcgcggcttg ttcctcccgt
     2101 gatcattccg gagctattcc gggggaccgg atcggggctt cccgacgtca agagcgccgt
     2161 ccagcagagc gttcagcggc tgctgagctt gcagcttccg cacggtggat ttgcgttctg
     2221 gcctgacgac cagcgggccc atccgtttgg ttcggcatac gcgacctggc tgcttcacaa
     2281 agcggcgcag gcgggacatc cggtgcctag cgatgcgctc cagcgagcaa cgacgtactt
     2341 gcagcgactg ctggcctcgc cgcagccagg aacagaggga cagctgctag agcccgtgac
     2401 ggtggcgcag gcaattatgc tggcgcggaa agaggccgag cagccgggtt cgacggtgac
     2461 gccgctttcc tttgattact ttgacaatac gcaacgggcg cttgtggcgt ttgtgctggc
     2521 ggaaatggga caagcgaatc cggcggcaaa tggcgtgctg tttgcgaacc gggcgatgct
     2581 gcctctgttt gcgcgggcgc tgctgctttt gacgctgcac aagacggcac ctgaggatct
     2641 gcggattgcg acgctgaccg aagaagtttt gtcaacggtg agcgcgagcg agcgaagtgc
     2701 gcatgtgcgt gagcagctgc cgtacaactt ggatgcgctg tttcattcgc cggtgcgaac
     2761 ccaggccgca gtcctgatgg cgcttttgcg ggtgcagccc aaaaatgcca acttgctgaa
     2821 gctggcgcgc ggacttctgt tcgagcgcgt gcaaggcgca tggaaaagta cgcaggacaa
     2881 tgcgtgggct atcttggcac ttctcgacta taaccggctg caccccgtcg agggatcaga
     2941 catcagcgcc ggtgcgtggc tgctcgatga acagctgatg tcggcgaaga tgccttcgcc
     3001 gcagagcccg ccgcagcgcg tcgattttac gctgcaaaag atgcttcagc ttgtgccgcc
     3061 cgatgaaggc aagcagtcgc ttatcgagct gcctctgacg ttgcagcgca gcggtcgcgg
     3121 aacgatgcat taccgcgtcg gtctggaatg gacgaccgcg cagaccggaa tgggcgcaac
     3181 gtcacaaggc atcaagttcc ggcgcgccct gcgaaccgcg gcaggggcac ttccgtcgac
     3241 gggatcgctg cttgcggggg aagcggtggc gatggacatc gacctgtctt gccagcagcc
     3301 ggtacatcat ctggtgctcg acattccgct gcctgcgggt ctggaagcca ttcagcgcga
     3361 aggggcggcg accagctccg cggcgctggc cggtccgcgc agtggtcaga tttcgtttga
     3421 ggagattcgg cgcgaccgcg tgctgatcta ctttgatgcg ctgccggcgg gcgaccatga
     3481 gcatacgctt cagctccggt cgacgacagc gggtcggtac atggtgccgc cagcgaaagc
     3541 gacggcgctg tacgatccgg gtctgcaagg cagcaccgac gcagtgaaca tctgggttca
     3601 ctaacgttcg atcgaatctg cggcggtgcg tctgtggatt agttggtgaa tgccggtggt
     3661 gtggtggttc cgctgagcgt tcccaaagcg cggatgtgga tgcgaccgag tccgccgttg
     3721 ccacctggat aggttgctcc gcacgatccc gcgttgccgc ctgctccggc gctcgcgacg
     3781 atcttgccgg tgttgagcgt ggcgctgtcg gcggtttgaa tgcgaacggc gccgcccgat
     3841 ccaccgccgc cgttgcccat gccgcgaccg ccaccgccgc agggaccgct gttcgatccg
     3901 ttgccgccgt ttgcgccgct gacggtgatg ctcgcagttc catccagggt gacggatttg
     3961 ctgttccaca tcacgatgcc gccaccgttg ccgcccggac ccggataggc gccgtcttca
     4021 tcggggccgc cctcaccacc cgcgctaccg aggaaaatgc tctggaacag atcctgtcca
     4081 cccaccgcgg tgccagcgag tccgcctctg tgctggccgc cgacgacgat gcaggccccg
     4141 atcgttccgt cgacgccatt tgtacccgcc gtgccatagg aaccgccgcc gcccgctgca
     4201 cagtcttggc cacgcgaacc tccaccaccg ccaccaccgt tgttcatgcc ctgtgcctgg
     4261 ccgttgagcg tcgaaaacgc cgaagcgccc aacgacgatt caccggtgcg tccgtgattg
     4321 agcgtgcagc cgggagcgct tgggaaacag acacccgtga cgccagcacc acggaatccc
     4381 ttgctcgcca tggtgatggt gccgccggcg gcgacatcgc ctctgctcag gaagaccaag
     4441 atgccgcctt tttgtccatc ccatgccgga gccgtcagga atccgcccgc cgtcacgttg
     4501 acgctgttgt attgcggcac ggccacaacc tgtgcgccgg ttacgtagtc gtatttgagc
     4561 ggatttttga aagtcagctg cgtgccgctc accgtcgcga tgcggttcat ttcatgctgg
     4621 cctgcgccgg ttccttgggt ctggtgaatc agcaccactt ggtcgaccaa aaatgggctg
     4681 ccatcgcgca ccgtcatggt ggtgccgccc atggttccgg tgccgctcgt tgctgcggta
     4741 ttgatggtcc gcgtcattcc gctgggaatc gccaggtcgc catctgcacc gttgcccaga
     4801 ccacagaccg gggtctggcc ttcgggaacc ggcgttccac aggtgaaaat gccacagtct
     4861 ttgtcggcca cgcaggtctt accgacggcg caggcgttgc agcttccgcc gcagtcgacg
     4921 tcgctttcat cgccgttttt gattccatcg gtgcaggacg gcgccgcgca aacaccgctt
     4981 gcgcagttgg cggaagtgca gtccgccgca accttgcaag cggcgttggg cgcgcacttc
     5041 atacaggctc cgccgcagtc tttgtcggtt tcactgccat cgagaattcc gtcggtgcag
     5101 gtcggcgcga tgcagacgcc cgcgggacag acaaggcccg actggcagtc ggttccgttt
     5161 tggctcttgg cctgcgcgcc gcacttggtt gaacacgagc cgccgcagtc gatgtccgtc
     5221 tcgccgccat tttggacttt gtcggtgcag cttggggtcg cgcagtcggt tccgcccgtg
     5281 cactgaccgc ttgttccacc gctgtcacag acggaaaaca caaatccaga gatgccggcg
     5341 agcagcatcg gcaaaaaacg cttccacgac tttttcattg attcccccct aacgtcataa
     5401 gtggcgcggc tttctagatc gccgcatcac gagtgtaggc gagggtttgc gggttcggca
     5461 cgcgattttt ccgattctcg tcgaagcaga gccattccca accgcttttg ccatggccca
     5521 ccgtaaagcc ttggttcgga aaaaaaatcg tgcatagatg cgcggcatgt cgctgcttgt
     5581 tcttgcttct gcttcgcctc gacggatgga tatgttggcc gctgcggcgg tgcctcatgc
     5641 ggtgtggcct gtcgacattg atgaaacgcc acagccgggc gaaacgcctg ccgcgctggt
     5701 ggaacgactg gccgaggaaa aagcagtggc ggcgtcggcg ttgtgtcctt ctggatcgcc
     5761 gattttggct gccgatacga tcgtgaccgt ggatggcgcg ctgcttggga agcctccgca
     5821 cgagggcgcg gcgcgacaga tgttgcggct tttgtctggc aaaacgcacg aggtgctgac
     5881 cggctatcac ctgcgctatg tgaatcgcca aggcgaatta cagaagcttc gcagagtggt
     5941 gactacgcaa gtgacagtgc gcgagcttac cgacggggac atcgcaggtt atctgcgcag
     6001 tgaggaatgg cgcggcaagg ccggttccta tgcaattcaa ggacgctttg cggcgtttgt
     6061 ctgcgcactc tctgggagct atgaaaacgt cgtgggactg ccgctgtgtt cggtccttgt
     6121 cgacctgcgg gcggcggatc tgcttcccgc cgactggccc agctggacaa gcggatgaca
     6181 ggttctgtgc gggactagta aggcgcgcag ctgcagatcg acatccgacc aggaatcgtg
     6241 gtgcgccagc taccggtcca gcgtccgggt tgacacaccg ccggacagcg ctgggccgca
     6301 tggtcgttgt tccagatcgg gccggcttcg acctcgcgta caaccggagc gggtggcggt
     6361 ggcgggggcg gcatcggtcc gggcggcggc atcggcacgg gcccaggttc ctggcgttcg
     6421 ctaaaacaca ggaagttgtc atgccaggtg tgggggtcgg acggttcgtt ccacgcgacg
     6481 cacgcgagcc ctggaatcgg accggaatag ctccactgca agcggtacgg agatccgtgc
     6541 ggcaagcaca ggaagttgtc attccaggtg tgcgggtccg ccggttcgac gatttgcgtg
     6601 cagcgcatgc ccggaatcag acccgagttg gaccacttca ttccgagcgg acgatcggaa
     6661 cagaaaaagt tatcgctcca ggtgtgcgga tcggcgggct cgcggatctg ttcgcagatc
     6721 atgccgggaa tcggacgcgc acagctccag gcaaacggtc ccaagcgcga ctcgcacgtc
     6781 agtggtgctt gcgcgggagg agcctgtggc gcgaccggtt cgatgatcca ctgctggttg
     6841 ggtccaaagt gacagtccca gctctgaacg tgcgcgcggt tttgtccaat gtcgggacca
     6901 tgcacatcga gacagcggtt gtccgcttgc acaatcagcg cccctcgttc gtgccgccag
     6961 tgctggttgg gcccaccgtg acaatcccac acttgaatcc gtccaccgtt gtccccggtg
     7021 tccggtccgt tcagatcaag acagcggttg ttggccatgc tgacgatccg tccacgatcc
     7081 cagcgccaca actggttcgc ggcccccgtg cagtcccaga gctggacgcg accgccgttc
     7141 accttcacct ggggtccatt taggtccaga catttctgtc cagcgcgaat cgcaaatgga
     7201 ccatggtgct gcgcctgcgc cgcagagatc gatagcaggt tggcgacgat gaatgcaccg
     7261 ctgacaagca cgctgcgttc tttcataaag gggccccttt ccctcaagag cgtgctcgcg
     7321 aacagagcag cgctcaatca agatagtgta ccgggcctta cggcgttgtg gcagaaaaaa
     7381 cacaccgcgc tttccctcga ggtcggtacg tgtggaagga aaacgcgaag gctgcgagcc
     7441 cacacaaacg gcaaagcagc gcgggcggaa gccgtgcggt ttttttgaaa ggtgggctag
     7501 ccggccaggc tgctgagatc gcgcgacagg acgcgattca gcgacgacag aagcgcaccc
     7561 ggttcgggga ccgaaccttg ggccaaatac gccagctcaa gcagcagatc gctgtaggtg
     7621 ttgatctctt cgctgccggg gcgcacggcg agcagatgcg ccagcttttc gacgatcggg
     7681 ctctgcggat tcagttccag aatcggtttt ttgacttcga ctttgcgacc cgcgcgctgc
     7741 agaagctgtt ccagatgcgc acccaagtct ccctcttcgg cgacgagaac cgacgcactg
     7801 tcagtcagcc gtgaagacag ccgaacttcc ttgacccgct cttggaaacg cacacgcaga
     7861 tgcgccagca gtccctggag ttctgtctgt tgttccggag tctgtttgtc cgcttccggc
     7921 tcgctctctc cctcaatctt gggaggcgtg aagtcacctt tgtgaatcgc gcggagtttc
     7981 ttgcccgaat actccgatag cgactgcacc acccattcat caatcggatc gatgaacagc
     8041 aggacatcga gctttcgctt gcgcagcgct tccacatgcg gactccgttc caccaccgtg
     8101 cgcgacgggc ccgtgatgta ataaatctca tcctgaccag cgggcatttt cgcaatgtag
     8161 tccgcgatgg tgatgcgctc tgcggcttct gtgttaagcg aagaccacag acagacttcc
     8221 gccacttttt ctttgtttgc gtggtcgagg aaaattccct ctttgatgat cccaccaaac
     8281 tcttcgaaaa acgcgcggta gtcttccggt ttctgttcgc gcagacgctt cagttcttcc
     8341 aaaacctttt tgacgatctg tttctgaatc gtcgtgatga tctggttctt ctgcaaaatc
     8401 tcacgcgaga cattgagcgg aagatcatcg ctgtcaacca caccacggac aaagcgcagc
     8461 cacggtggca gaagctcctc tgcgtgatcc aaaatgaaga cccgccgcac gtacagctgc
     8521 atgccgcgac ggcgattttg gtccatcatc atgtcaaacg gaggcttctt gggaatgaac
     8581 acgatcgcct ggaactgata gggcgtatcg atcgacagat gcagtttccc aaacggaacg
     8641 tcgtcagcgg aaaagccacc acacagctct ttgtaaaagt cgctgtattc ctcttccttg
     8701 acttcgctgg gtctttttga ccaaagcgcc gtcgcccgat tgagctgttt cccatcgagc
     8761 cggaccggcc acttcacgta gttcgagtac tgtttgatga tgtgctcgat ctgccacgac
     8821 tccaggtatt cgctggcatc gtcatttagg aaaagcttga ccgtggtgcc tggcgtggtg
     8881 cgcgttcctt cgccgagttc atacgttcct ccgccatcgc agcggaacag aatcggctgg
     8941 gaatcgggct cggcagaccg cgtatcgact tcaactttgt gcgcgaccat cagcaccgaa
     9001 taaaagccga caccaaactg accaatcaca tcgagcgact ttgccgcatc ggtgccttgt
     9061 tctttgagcc gcttgatcag tgcgccagtt cccgatcgcg caatcgtgcc gatgttgtcg
     9121 atcacttgct ggcgggtcat gccgatgccg gtgtcactga tggtcagcgt cttgtcgtct
     9181 ttttggaccg cgatctcgat cgctgattct tgtgccgata ccgcatccga cagttccggg
     9241 cgggtcagcg ccaaaaatcg cagcgtatca atcgcgtccg aggcattgga caccagctct
     9301 cgcaagaaaa tctctttctt gctgtagagc gagtggacca tcagatgcag aagctgctgc
     9361 acttcggtcg aaaacgtgcg ggtttcagtg gctttcggtt cggtttggct agaagtcatg
     9421 gagaaatggc taatcaccgt gcgcgtgact cgcaagggga agttttgcca agctgacagc
     9481 ctggcctgaa tttgggcaag ctgtgatacg atccagacat ggaatcaaat acaccgtccg
     9541 atcgagacac agaggacagc ccaagcggcg cgggcccaag cgatccgctc ctgtcgcttc
     9601 agatagtgtg ggtggcgctc cttgtgtcgc tgggaatcta cgcggtgatt gcgtacaccg
     9661 tggcaaagca ggtcgcaaac ggggcgcagc ttgcaccgct gccggcgatg cagatggcgt
     9721 ttgcgatgat ctcgctcagc tctgtgacgg tggcgtatgt gctgtcgcgc aagctgctca
     9781 agcaggcgat tggaaccgat gatccggcga cactgcccgt tgcaaagctg gcccggctcg
     9841 cgtttacgcc gtggatactc cggatggcga tgacagaatc ggtgggaatc tttggtctgt
     9901 tgatggcgat gttgaccggc cagccgtcga gcggactgcc gttcctgatc gtttccgcgc
     9961 tcgtgatgct gacgcacctt ccgaacgaaa aagcactccg caccgcggcc cagcagtagt
    10021 tcaattcccc cgttttgccg cacgttcgca gactccgttg tcaaagtggc acggaaccgc
    10081 cagcgacgag cgatcatttt gtcgcagcat accagcccag ctttgcacat gacaaagtga
    10141 agtcagcgga atggctggga ttctcccctc gtgacacttc gcagcgcgcg gtggcacatg
    10201 cttcgcaatc gttgtctgtc gcaggtgaaa cggctgatcg acaacgcgag gggggcgaac
    10261 atgcgtgaca aaaagcacac cacagggatg ttgggaatgg ctttggctgc ggcgctttct
    10321 tggtcttttc cagcgcgagc agagactcca gcagtaccgg acaagctggg tcgccacatg
    10381 gtcaatgcga gggtggccgc cgagctgctg actggctggg acagtggcat gggatggact
    10441 gttcccgtcg ttgcaattga acccgccatc ggtgtcacat cttcacgcaa tgggtatgtt
    10501 gtctttccct tttctgttca gaattttttc aatgcgacgg cactgggaat cggaatcggt
    10561 tatcagcacg acattccgct tccgatccgc aggctctatg caaccgcacg agcgaccgtc
    10621 tcatatgaga gctatcaaat ccgcaacggg tccctattca acgggtcgat cacacactca
    10681 cacaccgctg ccttccagcc atccatcggt gtgaagtacg tgcttgccgg gcgcgtgaat
    10741 ctgggaattg attttgcgag catcaagctt cggatacaga acggacttta cgacgcgagt
    10801 taccagtttg ttggctctgc gggcgtcacg ctgtgacgtt ccgacgctgc ggacgcggtg
    10861 aatagatgtc gtagcgaatg tcgcgcggca gattccatcc cgatggatcg ggctcggaca
    10921 gctcgctcag atcaacatcg gccatcacaa gtccaggtcc agcggaaatt tcgggcagga
    10981 ttccctgcgg tccacagaag taactttccc cgaaatactc gatcgtccat ggcggttcct
    11041 gacccagccg attcgatcca ccgatgaaga ctccgtggcg aaccgcgtcg acgaaaaact
    11101 cttggtgcca gaccttctga ctctttttcc ca
//
LOCUS       DAORGN010000167        11055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2492_length_11055_cov_6.412364, whole genome shotgun sequence.
ACCESSION   DAORGN010000167 DAORGN010000000
VERSION     DAORGN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_11055_cov_6.412364"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKL17_16140"
     CDS             <1..721
                     /locus_tag="PKL17_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46316.1"
                     /translation="AKKKDKGPPPVQHDPLETELNPQPPSNPSDGSTGSGANSGGSSG
                     SSSSGGSGGSSSGGSSSGGSSSGGGSSSGNSSGSSSAPATGKIGRYMVSLKLGAAPCV
                     YFSGGCSTLHQGVVGAEFAWALTPSAAAYLIVPIQVQFRQSVGTLMIPVGIQYDIGLP
                     VPNLYIYPRISLGYAALLVGAAGSLIADHFGVAIPEVGLKYVFSGRYHLGGEVVSLPV
                     FFSSSTVLVHYRAALSFGVSF"
     gene            complement(730..987)
                     /locus_tag="PKL17_16145"
     CDS             complement(730..987)
                     /locus_tag="PKL17_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46317.1"
                     /translation="MPIYEYECECGARLESLESLGTVRTCCGELCKKPEGAGGKGHGT
                     VQKVMSLSGIRGDGKEAKEPVYSPVKRQARPGCEDCDCSIT"
     gene            1122..1883
                     /locus_tag="PKL17_16150"
     CDS             1122..1883
                     /locus_tag="PKL17_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK46318.1"
                     /translation="MIALGSIRKLHFDPQKPPVLDGISLRIQKGETCGLCGPGASGKS
                     LLLKVICGLLRPEAGTVEVGGVSLHQASDDELRATQAAIGYLFQNNALFDSLTVFENV
                     AFPLRQRARLSQAIVDESDLRQRAEHALRGVGLFHHAGKMPSQLSGGQRKRVALARAV
                     VTRPPLLIYDEPTAGLDPVTTAKIYDLLHAERELTQATTLVVSSDIDALRRFAPRMLM
                     LYRGGLCYDGPSDRMEQSESAVVRQFVRGDLEGPL"
     gene            1880..2650
                     /locus_tag="PKL17_16155"
     CDS             1880..2650
                     /locus_tag="PKL17_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK46319.1"
                     /translation="MTRGLLWIGKLALSLCREVGGMALLLAYTIAMLLRGKFDGRELV
                     KSLYKMGVVSVPIVALTALFTGGTMVIQSGIFVQRFQAYGLLGWGTGYAVLREVGPIL
                     IGLMFSGRVGANNAADLGSMVVTEQVDGLRALAIEPIPFLIVPRVLAMMIAMFCLLVL
                     GDAVAIVGGMLFGKLLLGVDFASFYASLVDSIKLWDIAVGLLKGTFFALMIALTSCHY
                     GLNVKGGAVGVGHAVNDAVVAAALLIMVSDDIWTMVLR"
     gene            2647..3474
                     /locus_tag="PKL17_16160"
     CDS             2647..3474
                     /locus_tag="PKL17_16160"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK46320.1"
                     /translation="MTKKSAAKTTASSSPDKLAAPEARWGRFLLDAAYQLWEIALLTF
                     VGALRSRRPEGELARQLYQVGNRSLLFVLVTLGFTGMVLVFQSCLQLSRVTGDLSQVG
                     GEFIKYLIHEFGPSLTAMMLATRVGAGIAAEIGSMVVTEQVDALRMCGVEPVEYLLVP
                     RFLATQVMTLVLAVFGVLSAVLMGAFTAHQTFHVPYAVFLDLSKVRLPDVLTGVLKAL
                     AYGAAIPIVSGFCGLRTHGGSEGVGWATTRAVVSSSFAVIVLDFLISALALLTWQAL"
     gene            3644..6739
                     /locus_tag="PKL17_16165"
     CDS             3644..6739
                     /locus_tag="PKL17_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HNK46321.1"
                     /translation="MKRLLKLGVVGVAACLALALTVSRRGPAMMVGLGRTIGAPSERK
                     QYDLHNRLTTFNQTLGRVLEAYVDPTRVDPKQMLLSALDSIQKQVAEVMIEPFPEQNR
                     VVVHVDTAVREFGIENVDSPWAMAPKMQEIYDFIIQHLLPGTDNETLRNIEYAATNGM
                     LSTLDPHSLLLDPQTYQEMKVSTSGRFGGLGIVIRIVNGALTVVKPMKDTPAWDAGVR
                     KNDRIVKIDKEWTANMALNDAVQRLRGDPDTKVEVWFERDGDKQTRKIVLKRAEIRVQ
                     TVTSRLLPSSTGPVGYVRLSQFSQKSDDELKQALSDLGSKQPLRGLVLDLRHDPGGLL
                     DKAIKIADEFLESGTIVTTVGYANKRREETRAEPAAQPHIPMAVLVDGQSASASEIVA
                     GALKNLDRGVIIGSRTFGKGSVQVLMDNPDGSALKLTIAQYLTPGDLSIQSVGIMPDI
                     VLEPMSVDKDRLALFRTYKGTREQDLDAHLTSQNARANDKPVETLRYIWVDPKKAAPT
                     KAKGALLTDTDKKGSGEPGEEDDDDEAVLPPDDEEFVEDYDVQYARDLLASVVGYRRH
                     EILSSAKDFFARRQASEMSRLIDSLRKLGIDWAPVGQQAAAPELVATVTTDKPQHLAK
                     AGETIRFTATLTNRGQGIAGQVRAQLKSDNYLYEEREFVFGRIAPGETKTWSIPVKVP
                     KDTLPRLDVVKVQFFEEHGAAPPQQVLKVQLQGMERPRFAYSYQFIDDGIDSNRDGLL
                     QKGETVRLRVLIKNTGVGKAAKPFALLHNRSGEGVMVNKGRYELEAMAPGEQRAMDFT
                     FEVKNDFAEQMARVELQVWDFALHEGVTDKLSFPIVENKPVVEPRSGTVVTSKELVVR
                     SGAASNATEVGRVSGGARFKVTGRVADFVRVELEPNRPGFIPSQLVSETMSPPESNPP
                     LQLVWQATPPLLDVKAPLAVEQPSLRLPVSVRDDEQVLDGYVVVSNRVAKIEHRKVFY
                     RSNRNGPLAKEMQFDASVPLWPGVNIVTVVARQSGQVQSLHTMVINRLTDEPKTAQIA
                     R"
     gene            6811..7824
                     /locus_tag="PKL17_16170"
     CDS             6811..7824
                     /locus_tag="PKL17_16170"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNK46322.1"
                     /translation="MHAAATPIDLHTDTVKFISRGYDFFAEHDPSWMVRRFGGHVDLP
                     RLRAGNMAGLFFGIWTFPKPEVGCLAEAHRQIDALEKASAAGAFRICRSAEEIVACRQ
                     AGEVAALIGIEGGHSLERGENSRVVVERLQSLGQRGVRYLGLLHFSRNALGVPAMGLG
                     QDASQGLTPLGCEIVDACREFGILVDLAHINRKGFFDAVARKPGPLLVSHTGVVGVTP
                     HWRNIDDKQIHAVADSGGVIGVIFAPQFVGRDDLDGVVAHLLHLLKIAGDDAVALGSD
                     WDGFIRPTKGLASPAELPNLTEALLRAGVSAATVHKILGENVLRLLRAAPPKLAASSQ
                     QNT"
     gene            7828..8733
                     /gene="metF"
                     /locus_tag="PKL17_16175"
     CDS             7828..8733
                     /gene="metF"
                     /locus_tag="PKL17_16175"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNK46323.1"
                     /translation="MRITERLQKPSPSFSFEFFPPRTPTGLAGLLDTLRDLKELEPSF
                     VSVTYGAGGSTQRSTTELTAQIKHEIGIEAMAHLTCIGHDQVALAQMLDELALAGIDN
                     VLALRGDRPSATSDPAARVNQPADPSGEEFAYASQLVRFIHSDRKRWPFCVGGACYPE
                     GHIECPSREDDLRHLAEKVELGTEFLISQLFFDNAFYFDFVQRAQAARIRVPIVPGIM
                     PITSFEQVERFTRLCGATIPMRLRLQLERYQRDPEAILELGVAHATIQCAELLARGAP
                     GIHFFTLNKSRATRMILTALRSAGR"
     gene            8833..10017
                     /gene="dnaJ"
                     /locus_tag="PKL17_16180"
     CDS             8833..10017
                     /gene="dnaJ"
                     /locus_tag="PKL17_16180"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNK46324.1"
                     /translation="MSSQKRDYYEVLGVSRTANDAELKKAYRRLAMEYHPDRRPGDKE
                     AEEKFKEAAEAYAVLSDAEKRGLYDRFGHQGPSQGGFQGGFGGIEDIFSAFADIFGGG
                     GFPGFGGGGRRVPRGDDIEIELSVTFLEAAKGVKRELEIDRHSACETCKGSGAKPGTQ
                     PQRCRTCNGRGQVAHQQGFFVISTTCPQCRGQGSTIAEKCGSCKGAGQVAKRATVSVT
                     VPAGIDDGQRLRLTGQGDASSQTGGEAGDLYVLIRVQADPRFVRDGDDLWTDIPLTFT
                     EAALGTRVEVPTPDGSETIDVAPGTQPLSKQVLPGRGMPNVRGRGRGNLVVRFVVLVP
                     KELSSEARELIEKLGPHLYSPRSASSQELPDHDASDDRHEDGHESHKESLFDRLFRKN
                     KK"
     gene            10144..>11055
                     /gene="bioB"
                     /locus_tag="PKL17_16185"
     CDS             10144..>11055
                     /gene="bioB"
                     /locus_tag="PKL17_16185"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNK46325.1"
                     /translation="MHSAIQTEAGSTATGQPQKPSIADVCAIYDLPLPTLVHRAMTVH
                     QANHDPSEVQLCSLLSVKTGGCQEDCGYCPQSAAHGSMDAEAMLSVDDVLSKARLAKG
                     AGATRFCMGAAWRSVKDGPAFERVLDMVRGVRGLGMEACVTLGMLTEEQARKLGDAGL
                     TAYNHNLDTSRSFYGKVITTRTYDDRLETLQRVRRAGISLCCGGIIGMGESVADRCAL
                     LHTLASLDPPPESVPVNALVAVPGTPLEARPKVDPLELVRTIATARILMPKSRVRLSA
                     GRTELSREAQLLCFLVGANSIFYGDKLL"
BASE COUNT         2064 a         3139 c         3603 g         2249 t
ORIGIN      
        1 ggcgaagaaa aaagacaagg gaccaccgcc ggtccagcac gatccgctgg aaaccgagct
       61 caatccgcag ccgccgtcga acccgagtga cggtagcacc ggaagtggcg caaatagcgg
      121 tggttcgtcg ggttcgtcgt ccagtggcgg atcgggcggt tcgtcgtcgg gcggttcgtc
      181 gtcaggcggt tcgtcgtcag gcggcggttc gtcatcggga aacagcagcg gatcgtcgag
      241 cgctccagcg acgggaaaaa ttggacgtta tatggtcagc ctcaagcttg gggccgctcc
      301 ctgcgtctat ttttccggtg gctgtagcac gctgcaccaa ggcgtcgtcg gcgcagagtt
      361 cgcctgggcg ctgacgccat ctgcggcggc gtatctgatt gtgccgatcc aagtccagtt
      421 tcgccaaagc gtcggcacgc tgatgatccc cgtcggtatt caatacgaca ttgggctgcc
      481 tgtgcccaat ctctacatct atccgcggat cagcctcggt tatgccgcac ttctcgtcgg
      541 ggccgcaggg agcctgatcg ccgatcactt cggcgtcgcc atccccgaag tcggtctgaa
      601 atacgtcttt tcgggtcgct atcacctggg cggcgaagtc gtcagcctgc cggtcttctt
      661 ttccagcagc accgtgttgg ttcactaccg cgccgcgctg tcgttcggcg tgagctttta
      721 gcgaaaccgc tacgtaatcg agcagtcgca gtcttcacag ccgggacggg cttgccgttt
      781 caccgggcta tacactggtt ctttggcttc cttgccgtcg ccacggatac ccgaaagcga
      841 catgactttc tgcacagtgc cgtgtccttt gccgccggct ccttcgggct ttttgcacag
      901 ctcgccacag cacgtccgga cggttcccag cgactcgagc gattccagtc gggcgccaca
      961 ttcgcactca tattcgtaaa tcggcatatg gggaaggtat tcacacaatt cggtctgtca
     1021 aaccgcaggc ttcggtgctg gtctggactc gtcggcagcg gcgcttctga cgccaaaaaa
     1081 ccttgcacga cgggccgcga gtgggcgata agctcgtcgc tgtgattgcc cttggcagca
     1141 tccgcaagct gcatttcgac ccccaaaaac cgccggttct cgacgggatt tcactgcgga
     1201 ttcaaaaagg cgaaacctgt ggtttgtgcg gacccggtgc gtcgggaaag agcctgctgc
     1261 tcaaagtcat ctgcggtctt ttgcgacccg aggccggtac agtcgaagtc ggcggggttt
     1321 cgctgcacca ggcttccgac gatgagctgc gcgcgaccca agcggcgatt ggctatctgt
     1381 ttcaaaacaa cgcgctgttt gactcgctga cggtgtttga aaacgtcgcc tttccgctgc
     1441 ggcagcgggc gcggctgtcg caagcgatcg tcgacgaaag cgatctgcgg cagcgggccg
     1501 aacatgcgct gcggggcgtc ggacttttcc accatgccgg aaaaatgccg tcgcagcttt
     1561 cggggggaca gcgcaagcgg gtggccttgg cacgggcggt ggtgacgcga ccgccgctgc
     1621 tcatttacga cgagccaacg gcgggacttg atccggtgac gacggcgaaa atctacgacc
     1681 ttttgcacgc cgagcgagag ctgacccaag cgacgacgct ggtggtgtcg agcgatattg
     1741 atgcgctgcg tcgttttgcg ccgcggatgc tgatgcttta ccgcggcgga ctctgctatg
     1801 acggtccgtc ggatcgcatg gaacaaagtg aaagtgcggt ggtgcggcag ttcgtccgtg
     1861 gcgacctcga gggtccgcta tgacgagggg gcttttgtgg attggcaagc tcgcgctctc
     1921 actgtgtcga gaagtcggcg gcatggcgct gcttttggcc tacacaatcg cgatgcttct
     1981 gcgcggcaag ttcgacggac gagagcttgt caaaagcctg tacaaaatgg gcgtcgtttc
     2041 ggtgccgatc gtcgcgctga ccgcgctttt taccggcggc acgatggtga ttcagtcggg
     2101 cattttcgtg cagcgctttc aggcctacgg gctgctcggt tgggggacgg gatatgcggt
     2161 gctgcgcgag gtcgggccga ttctgatcgg gctgatgttt tcggggcgcg tcggggccaa
     2221 caatgcagcg gatctcggtt cgatggtggt gaccgagcag gtcgacgggc tgcgggcgct
     2281 ggcgatcgag ccgataccgt ttcttatcgt gccgcgcgtt ctggcgatga tgatcgcgat
     2341 gttttgcctc ctggtgctcg gtgatgcggt cgcgatcgtg ggcggaatgc tgtttggcaa
     2401 gctgctgctg ggcgtcgatt ttgcatcgtt ttatgccagc ctggtcgaca gcatcaagct
     2461 gtgggacatc gcagtcgggc tgctcaaagg gacatttttc gcgctgatga ttgcgctgac
     2521 gtcgtgtcac tacgggctca acgtcaaagg cggggccgtc ggtgtggggc atgcggtcaa
     2581 tgatgcggtc gtcgctgcgg cgctgctcat catggtgtcc gacgacatct ggacgatggt
     2641 gctacgttga ccaaaaagtc ggctgccaag accaccgctt cgtcatcgcc agataagctc
     2701 gctgctccgg aagcgcgctg gggtcgtttc ctgttggatg cggcatacca gctttgggaa
     2761 atcgcgttgc tcacgtttgt cggggcactg cgatcgcgcc ggcccgaggg tgagcttgct
     2821 cggcagcttt accaagtcgg caatcgctcg cttttgttcg tgctggtcac gctcgggttt
     2881 accggcatgg tgctggtgtt ccaaagctgt ctgcagctgt cgcgggtgac gggcgatcta
     2941 agccaggtcg gcggcgagtt catcaagtac ctgatccatg agtttggacc gtcgctgacg
     3001 gcgatgatgc tggcgacgcg ggtgggggcc gggattgcgg cggaaattgg atcgatggtg
     3061 gtgacggaac aggtcgacgc gctgcgcatg tgtggggtcg agcccgtcga gtatctgctg
     3121 gtgccgcgct ttttggcgac gcaggtgatg acgctggtgc tggcggtgtt tggggttttg
     3181 tcggcggtgc tgatgggggc gtttactgcc catcagacgt tccatgtgcc ctacgcggtg
     3241 tttctcgatc tcagcaaagt gcgactgccc gacgtgctga ccggggtttt gaaagcgctg
     3301 gcctacggcg cggcaattcc gattgtgagc ggcttttgcg gcctgcgcac ccacggtggt
     3361 tccgagggtg ttggctgggc aacgacgcgc gccgtggtgt cttcgtcgtt tgcggtgatc
     3421 gtgctcgatt ttctgatttc agcgctcgcg ctgcttacgt ggcaggcgct gtagttggat
     3481 ttttttctcg acggaaaaca ccaccatggg gatgcgcatt ttgctgcggc atcctaaaag
     3541 aaaacggaca gtctgttttg cccgagcggc ccgagtgatg gataattttc gtggtgttgg
     3601 acagggagat cgcaaggtgc ctgatgagac gccggagcta cggatgaagc gtttactaaa
     3661 gttgggtgtc gtcggtgttg cggcttgttt ggcgcttgcg ctgacggttt cgcgacgtgg
     3721 tccagcgatg atggttgggc tgggtcgcac gatcggggct ccgtcggaac gcaagcagta
     3781 cgacctgcac aaccggctga cgacgttcaa tcagacgctg gggcgagtgc tcgaagcgta
     3841 tgtcgatccg acgcgggtgg accccaagca gatgctgctg tcggcgctcg actcgattca
     3901 gaagcaggtc gccgaagtca tgatcgagcc gttccccgag caaaatcgcg tcgtggtgca
     3961 cgtcgatacc gcggtgcgtg agtttggcat cgaaaacgtc gattcgccgt gggcgatggc
     4021 gccgaagatg caggagattt acgactttat catccagcat cttttgccgg ggacggacaa
     4081 cgaaacgctc cgcaacatcg agtacgcagc gacgaacggc atgctgtcga cgctcgatcc
     4141 gcactcgctt ttgctcgatc cgcagaccta ccaagagatg aaagtctcga cgagcggacg
     4201 gtttggtggg ctcgggatcg tcattcggat cgtcaatggt gcgcttaccg tcgtcaagcc
     4261 gatgaaagac acaccggctt gggatgcagg cgtccgcaaa aacgatcgca tcgtcaaaat
     4321 cgacaaagag tggaccgcca acatggcgct gaacgacgcg gtacagcgtc tgcgcggcga
     4381 tcccgatacc aaggtcgaag tttggttcga gcgcgatggt gacaagcaga cccgcaagat
     4441 tgttctcaag cgagccgaaa ttcgcgtaca gacggtgact tcgcggctgc ttccttcgtc
     4501 gacaggtccg gtcggttatg tgcggctgag ccagttttca caaaagagcg acgatgagct
     4561 gaagcaggct ctctcggatt tgggtagcaa acagccgctg cgtggtctgg tgctcgatct
     4621 acggcacgat ccgggcggac tgctcgacaa agcgatcaaa attgccgacg agtttttgga
     4681 atcgggcacc atcgttacca ccgtcggtta tgccaacaaa cgacgcgaag aaacgcgagc
     4741 cgaaccggct gcgcagccgc acattccgat ggcggtgctg gtcgacggtc agtcggcgtc
     4801 cgcgtcggag atcgtcgcgg gtgcgctgaa aaatctggat cgcggtgtga tcatcggcag
     4861 tcggacgttc ggcaaaggct cggtccaggt gctgatggac aaccccgacg gatcggcact
     4921 caagctgacg attgcgcagt atctgacgcc aggcgacctg tcgattcagt cggtgggcat
     4981 catgccggac attgtgctcg aaccgatgtc tgtcgacaaa gatcgcttgg cgctgtttcg
     5041 aacctacaaa ggcacgcgcg aacaggattt ggatgcgcac ctgacgtcgc aaaatgcccg
     5101 tgccaacgac aagccggtcg agacgttgcg ctatatctgg gtcgatccca aaaaagcggc
     5161 tccgacgaaa gccaaaggag cactgctcac cgacaccgac aaaaaaggca gcggcgagcc
     5221 cggtgaagaa gacgatgacg acgaagcggt gctgcctccc gacgatgagg agttcgtcga
     5281 ggactacgat gttcagtacg ctcgcgactt gctggcatcg gtggtgggtt atcggcggca
     5341 cgagatcctg agctcggcga aggatttttt tgcacgtcgg caagcgagtg agatgagccg
     5401 gcttatcgac tcacttcgca agctcggcat cgactgggcg ccggtcggtc agcaagccgc
     5461 agcgccagag cttgtggcaa ccgtgacgac ggacaagccg cagcacctgg caaaagcagg
     5521 cgagacgatt cgttttaccg cgacgctgac aaaccgcggt caaggcattg ccggtcaggt
     5581 tcgcgcgcag ctcaagagcg acaactatct gtacgaggag cgcgagtttg tctttggccg
     5641 gattgcgccg ggcgagacca agacgtggtc gattccggtc aaagtgccca aagacacgct
     5701 gccgcggctc gatgtggtga aagttcagtt cttcgaggag catggcgcgg cgccgccgca
     5761 gcaggtgctc aaagtgcagc ttcagggcat ggagcgaccg cggtttgcct acagctatca
     5821 gtttatcgac gacggaatcg attcgaaccg cgacggactg ttgcaaaaag gcgagacggt
     5881 gcggctgcgg gtgctcatca aaaacacggg cgtcggcaag gccgccaagc cgtttgcact
     5941 tttgcacaat cgatccggcg agggtgtgat ggtcaacaaa ggccgttacg agctggaagc
     6001 gatggcgcca ggcgaacagc gggcgatgga cttcacgttc gaggtgaaaa acgactttgc
     6061 cgagcagatg gcacgggtcg agcttcaggt ctgggatttt gcgctgcacg aaggcgtgac
     6121 cgacaagctc tcgttcccca tcgtcgaaaa caagccagtg gtcgagcctc gcagcggcac
     6181 cgtagtgacc agcaaagagc tggtggtgcg tagcggcgcg gcaagcaatg caacggaagt
     6241 cggtcgtgtt tcaggtgggg cgcgattcaa agtgacaggg cgggttgccg acttcgtgcg
     6301 ggtcgagctg gagcccaatc gtccgggctt tattccgtcg cagctcgtga gtgaaacgat
     6361 gtctccgcct gaaagcaatc cgccgcttca gctggtttgg caagcgacgc cgccgctgct
     6421 tgatgtaaag gctccgcttg ccgtcgaaca gccgtcgctg cgcctgccgg tctcggtgcg
     6481 cgacgatgag caggttctcg atgggtatgt cgtggtgtca aaccgtgtcg ccaagatcga
     6541 acaccgcaag gtcttttatc gcagcaaccg caacggaccc ctcgcgaaag agatgcagtt
     6601 tgacgcaagc gtgccgctgt ggcccggcgt caacatcgtg acagtcgtgg cgcgtcagtc
     6661 gggtcaagtg cagtcgctgc acacgatggt gataaatcgc ctgaccgacg aacccaagac
     6721 cgcacaaatc gcgcgctagc cagcagtctt ttccctttca tcgacatgac ttggacgccg
     6781 aagatcccgc tcagcgacga agctcgcgcc gtgcacgcgg ctgcgacccc gattgatctg
     6841 cacaccgaca cggtcaagtt catcagccgt ggctacgatt ttttcgccga gcacgaccct
     6901 tcgtggatgg tgcgtcggtt cggtggtcac gtcgatcttc cgcggctgcg cgccggcaac
     6961 atggcggggc tgttttttgg catctggaca ttcccaaagc ccgaggtcgg ctgtctcgcc
     7021 gaggcacatc gccagatcga cgcgctcgaa aaagcgtccg ccgctggggc gtttcgcatc
     7081 tgtcgttcgg ctgaagagat cgtcgcgtgt cgacaggctg gtgaagtcgc ggctctgatt
     7141 ggcatcgaag gtggccacag cctggagcgg ggtgaaaaca gccgagtcgt cgtcgaacgg
     7201 ctgcagtctt tgggccagcg cggggtccgt tacctgggtc ttttgcactt cagtcgcaat
     7261 gcgctcggtg ttccggcgat gggcctgggt caagacgcaa gccaaggcct gacgccgctc
     7321 ggctgtgaaa tcgtcgatgc ttgccgcgaa tttggcatct tggtcgacct ggcacacatc
     7381 aatcgcaagg gttttttcga cgcggtggcg cgcaagccgg ggccactttt ggtaagtcac
     7441 acgggcgtcg tcggcgtaac cccgcactgg cgaaacatcg acgacaaaca gatccatgcc
     7501 gtcgctgatt caggcggtgt cataggtgtg attttcgcgc cgcagttcgt cggcagagac
     7561 gatctcgatg gagtggtcgc gcaccttttg cacttgctca agattgcggg cgacgatgcg
     7621 gttgcgcttg gatcggactg ggacggcttt atccgtccga ccaaagggct cgccagtccg
     7681 gcggagctgc ccaatttgac cgaggcgctt ttgcgtgccg gtgtttcggc tgcgacagtt
     7741 cacaagattt tgggggaaaa cgtgctacgt cttctgcgcg ccgcgccgcc caaacttgcg
     7801 gcctcgtcgc agcaaaacac gtaagccatg cgcatcaccg agagacttca gaagccctcg
     7861 ccgagctttt cgtttgagtt ttttccgccc cggacgccga cgggtcttgc cggactgctc
     7921 gatactttgc gcgatctcaa agaactggag ccgtcgtttg tgtcggtgac atatggggcg
     7981 ggcggttcga cgcagcgatc gacgaccgag ctgacggcgc agatcaagca cgagattggc
     8041 atcgaggcga tggcgcactt gacctgcata ggtcacgacc aggtggcgct ggctcagatg
     8101 ctcgacgaac tggcgcttgc cgggatcgac aacgtgctgg cgctgcgagg tgacagaccc
     8161 tcggcgacga gcgatccggc ggcgcgggtg aaccagccag ccgatccaag cggcgaagag
     8221 tttgcctatg ccagccagct tgtgcgcttt atccacagcg accgcaagcg ctggccgttc
     8281 tgcgtcgggg gcgcctgtta tcccgaaggc cacatcgagt gcccatcgcg cgaagacgat
     8341 ctgcggcacc tggccgaaaa agtcgagctt ggcactgagt ttctgatctc tcagcttttc
     8401 ttcgacaacg ccttttactt cgatttcgtg cagcgggccc aggcggcgcg gattcgcgtc
     8461 ccgattgtgc cgggcatcat gccgattacc agcttcgagc aggtcgaacg ctttacgcgg
     8521 ctgtgcggag cgacgattcc gatgcgactg cggcttcagc ttgagcgata tcagcgcgat
     8581 cccgaagcga ttttggagct gggggtcgca catgcgacga ttcagtgcgc cgagctgctt
     8641 gcgcgtgggg cgccggggat tcactttttc acgctcaaca agagccgggc gacgcggatg
     8701 atcctgacgg cgctgcgttc ggcgggtcgc tgagcggtgc ggttcggaac acaaatcgtg
     8761 taactggcaa aaattctctc ggagcactgc ttgacaggct gcttcggggc gttagctttc
     8821 tgttgctgtt ttatgagctc gcaaaagcgt gactattacg aagtgctcgg cgtatcgcgg
     8881 accgcaaacg atgccgagct aaagaaggcc tatcgtcggc tggcgatgga ataccatccc
     8941 gatcgtcgtc ccggcgacaa agaggccgaa gagaagttca aggaagctgc cgaggcgtat
     9001 gccgtgcttt ctgatgccga aaagcgcggt ctttacgatc gcttcggcca tcagggaccg
     9061 tcgcagggtg gcttccaagg cggcttcggc ggcatcgagg acatcttctc ggcgttcgcg
     9121 gacatcttcg gcgggggtgg tttccccggc tttggcggcg gaggccgtcg agttccgcgc
     9181 ggcgacgaca tcgaaatcga actttcggtg acgtttctcg aagcggccaa aggcgtcaag
     9241 cgcgagcttg agatcgatcg ccattccgcc tgcgagacct gcaaaggatc gggggccaag
     9301 ccgggaaccc agccgcagcg ctgccgcacc tgcaacggtc gaggccaagt cgcgcatcag
     9361 cagggcttct tcgtcatttc gacgacctgt ccgcagtgcc ggggccaagg ctcgacgatt
     9421 gcggaaaagt gcggcagctg caaaggcgcc ggccaggtcg caaagcgtgc caccgtaagt
     9481 gtcactgttc cggcgggcat cgacgacggc cagcgactgc gactgaccgg gcagggcgat
     9541 gccagctcgc aaaccggcgg cgaagcgggc gatctgtacg tgctgattcg ggtgcaagcg
     9601 gacccacgct ttgtccgcga cggcgacgat ctgtggacag acattccgct gacgtttact
     9661 gaagcggcgc tcggcacccg cgtcgaagtc ccgactcccg acggaagcga gacgatcgac
     9721 gtcgcgcccg gtacccagcc gctgtcaaag caggtgctgc cgggtcgcgg tatgccgaac
     9781 gttcgtgggc gcgggcgcgg caacttggtg gttcgcttcg tcgtcctggt gcccaaagag
     9841 ctgtcgtccg aagcgcgtga gcttatcgaa aagctcggac cgcatctgta cagcccgcgc
     9901 agcgcgtcgt cacaagaact gcccgatcat gacgcgagcg atgaccgtca cgaagacggc
     9961 cacgagtccc acaaagagtc gctgttcgac cgcctgttcc gcaaaaacaa aaagtagtct
    10021 ccgccgcgtc ggctgacttt ccccgcgtcc catgtttttc cggttctgcg ctgtggcctg
    10081 acgccaagca atcgccgctg cgcctttgcg gcgacatcca aaaagtcgtg ctataagccg
    10141 cgcatgcaca gtgcgatcca gaccgaagct ggctctacag cgacgggaca gccccagaag
    10201 ccaagcattg ccgatgtgtg tgcgatctat gacctgccgc ttcccacgct ggtccatcgc
    10261 gccatgacgg ttcatcaggc aaaccatgat ccgagcgaag tacagctctg ttcgcttttg
    10321 tcggtaaaga ccggcggctg ccaagaagac tgtgggtact gtccgcagtc ggcagcgcac
    10381 ggcagcatgg acgccgaagc gatgctttct gtcgatgatg tgctctcgaa agcgcgactc
    10441 gccaaaggcg ccggtgcgac gcggttttgc atgggggccg cctggcgcag cgtcaaggat
    10501 ggtcctgcgt tcgagcgggt gctcgacatg gtgcgcggcg tgcgcggcct gggcatggaa
    10561 gcgtgcgtca cactcgggat gttgaccgag gagcaagcgc gcaagctcgg ggacgcgggg
    10621 cttacggcgt ataaccacaa cctggatacc tcgcgatcgt tttacggcaa ggtcatcacg
    10681 acgcgaacct acgacgacag gctcgaaacg ctccagcggg tgagacgggc cgggatcagc
    10741 ctgtgctgcg gcggcatcat cggcatgggg gaatccgtcg cagaccgctg cgcgctgctg
    10801 catacgctgg cgtcgctgga cccgccgccc gaaagtgtgc cggtcaacgc gcttgtcgcg
    10861 gtgccgggaa cgccgctcga agcgcggccc aaagtcgatc cgctggaact tgtgcggacg
    10921 attgcgaccg cacggattct gatgccaaag agccgggttc ggctgtcggc gggacggacc
    10981 gagctttcgc gagaagcgca gttgctctgt ttcctcgtcg gtgccaactc gattttttat
    11041 ggcgacaagc tgctg
//
LOCUS       DAORGN010000168        11050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2496_length_11050_cov_6.064939, whole genome shotgun sequence.
ACCESSION   DAORGN010000168 DAORGN010000000
VERSION     DAORGN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2496_length_11050_cov_6.064939"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PKL17_16190"
     CDS             <1..1151
                     /locus_tag="PKL17_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46326.1"
                     /translation="MSAQAGGAGVLRLRAHVDIATRPPQRLDVDSRQFLKFRFGAAVE
                     YRQFFSRDARIGYPQQVNAQSDADLRIKDGDPLSFRLYNQFLVTNDARNLEVATTQTF
                     APRIFERLSLLGMYRPFGGPLEIGLSESLRVDHYVQSELSRGRSFQNDIALWGAYKML
                     PQTQLKLEVRSSWTSFYGDGAVIPNSAPLRITGGIQSLLFPWLGASLYVGYGNSLHYG
                     LFDTTQLVGDTSSYRYNNFVGGLEARLLLVPRMNLRAGWARDFFDSIYATYLRDDRLY
                     VAYEHNPWRSLWLSAGFETYLRVYGPLVAPSTLYYRAYKNGATTRSDTLFALNVDASY
                     RPLSWLQAGISYNLLADVTDFGFVDGAGTRMDAGFLKHVVLFKADLSY"
     gene            1165..1800
                     /locus_tag="PKL17_16195"
     CDS             1165..1800
                     /locus_tag="PKL17_16195"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNK46327.1"
                     /translation="MHSPQHKTQRRGLFSSRVLRKFAFGVWLTLGSGCPSTPAVIVRE
                     ATPPPQVDMSLGQDDVFEVRVYGEAELTGTYRVSADGTIDFPLIGRLAVRGQTTAQVS
                     DALTDKLRAYLKQPQVTVFLKEVHSKKVVLIGQVRSPGTINFVEGMTLEQAISMVGGL
                     TPMAARDRVRVTRVLGGKAETLVVNFKAIAEGAQTFFLQPGDTVSVDERLF"
     gene            complement(1801..2736)
                     /locus_tag="PKL17_16200"
     CDS             complement(1801..2736)
                     /locus_tag="PKL17_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46328.1"
                     /translation="MKTLLALSLLSLPVVCATASATPLRNQAGTYELSVMTDGAQAPT
                     YYHNGETYVMGQRGQRYTLRIHNHSGRRVEAVVSVDGRDVVDGKSADYRLKRGYLVPP
                     WGYVDVDGWRVSQGEAAAFRFSSVANSYVGRTGSTREVGVIGVAVFPERYVPPPPPPP
                     RPLYVPRHELYGHGGDDYSEERAPAAKSAPPSGGAPATSAPMSPMDERARAGGGALAD
                     RETMQRDKRPGLGTGFGERVSSPVEEVVFYRHNPVTPSVVLGVRYNDRSGLVAMGVDV
                     DGDGDNMHDVYLRRSAEPFVVPSRRYAEPPAGWPY"
     gene            complement(2808..3911)
                     /locus_tag="PKL17_16205"
     CDS             complement(2808..3911)
                     /locus_tag="PKL17_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNK46329.1"
                     /translation="MTTISLSRPSITDEEIKASERVLRSGWLVYGPENRAFEEELAAA
                     CGKRFCVTVSSGTAALHLGLLAQKLPLTSILSPALTFPATANVANFLPTPPQVHLADV
                     DADTFCLRQETVQAWLDSRRGPALVVHQFGYPSPLPVRNDDLLISDAACAIAVRPAMG
                     GKLACLSFHPRKLLTTGEGGAILTDDEQLCETLRRLRAHGLTSVPGEEVMELPASGLN
                     YRLSEPAAAIGRVQLRRLPQLVKKHRELAEQYLRNLADTGLGFQADHPDRIWQTFAVV
                     LPEGVNRPSLRAKLKEAGIETQIASYGLHRLAAFRTAPCFSETGGPLALAVSDRLHDR
                     GLALPLHSELSAADVHRVCQELKKALSLPEVCA"
     gene            complement(3908..4771)
                     /locus_tag="PKL17_16210"
     CDS             complement(3908..4771)
                     /locus_tag="PKL17_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNK46330.1"
                     /translation="MLFRRPDGILVDKLSPMRRMMPYILRTRNESCVYFEQKLDLSET
                     LPYLAKLNAQLGRSKTDEINFFHLLLAATARVFSERPELNRFVSGGKIYQRRHIELSF
                     AIKKERSDKGVLATTKLRFTGRESLADIASQTRGAVTVGRSDKKSQADHEMAILTRLP
                     GPLLRFIMWIQRSLDAWNLLPSALTESDPLYASLFFANLGSIGLDSAYHHLFEYGTVS
                     FFAVLGQLTHLPFVDGHASALTTRPGVTIKYTFDERITDGWYCARSLDMLKAYVEHPD
                     TLSEATVQDPT"
     gene            4770..5396
                     /locus_tag="PKL17_16215"
     CDS             4770..5396
                     /locus_tag="PKL17_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46331.1"
                     /translation="MAEDDTRRNYDIKSRVLGSVLRFCGVLAVGCLTSACAVTIPQDG
                     LCAPMANPTVDCQDGRGLGAVVVRWRMADLQLGRLLGRGLCCCNPSPSRDGIDRAQCD
                     QIGSSCLDSPAWLVEHVNLTLHRSDDDTEYSWDIPCSEGEFTTPYCIRPGLYDMKLTA
                     DINKLADDGSSRFLCAQKKAVSPPAVRRAIVEGQATNLDAVVLGINAP"
     gene            complement(5400..5762)
                     /locus_tag="PKL17_16220"
     CDS             complement(5400..5762)
                     /locus_tag="PKL17_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46332.1"
                     /translation="MASADSPPPPYWLLIAVLSSSLPLRPEVSLRLYQAAYRLHRSGE
                     NSERLQGDLLRGKVVRLHKDLAIGSVVGPAFEAEISTESGEGVVHFLLTRQGLELGDE
                     QAEKAPDTLPTPPGKWLN"
     gene            complement(5838..5912)
                     /locus_tag="PKL17_16225"
     tRNA            complement(5838..5912)
                     /locus_tag="PKL17_16225"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5877..5879),aa:Glu,seq:ctc)
     gene            complement(5977..6050)
                     /locus_tag="PKL17_16230"
     tRNA            complement(5977..6050)
                     /locus_tag="PKL17_16230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6016..6018),aa:Gln,seq:ctg)
     gene            complement(6244..6486)
                     /locus_tag="PKL17_16235"
     CDS             complement(6244..6486)
                     /locus_tag="PKL17_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNK46333.1"
                     /translation="MHADIEAKVKDIVCSQLEVSLDKVRPEASFIDDLKADSLAVVEL
                     VLELEQEFGIEIPDEDTEQIKTVQDAINYIKTHIKK"
     gene            complement(6725..7342)
                     /locus_tag="PKL17_16240"
     CDS             complement(6725..7342)
                     /locus_tag="PKL17_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNK46334.1"
                     /translation="MFARSARRKTERPPLTDAQLFEAAVSALATRSRSEAELRRILTR
                     KLDEPSRQRIDQVIARLHELGYLSDARLAENFVESRQKQSHLGRRRVAQELRRRGVNA
                     DLVEQTVERAYSEVDELQLALSFVEKKRLRITSSPGSSGSTSSRKSPQDAKVQKETAR
                     ALRALQRAGFSLATCWKVVRHMSDAAALAGLEECSDDSVASDSDL"
     gene            7416..7976
                     /locus_tag="PKL17_16245"
     CDS             7416..7976
                     /locus_tag="PKL17_16245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK46335.1"
                     /translation="MTAAPSLVTAPPAAYSVTEVYRLHAGDVARWAARLGGPQVDVED
                     VVQDVFLTVHRLLPEFRGEAKITTWLFRITQNQVRHRLRRLRLRRLFRSSDEDDVISE
                     LPSHGPSPIDDLERKEARAVVYRILDGMSEKYRTAFILFEIEKLPGEEIAQLLNQKVA
                     TIWVWLHRARAQFLEGLEKHNKSERS"
     gene            7973..9034
                     /locus_tag="PKL17_16250"
     CDS             7973..9034
                     /locus_tag="PKL17_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46336.1"
                     /translation="MKTTSPYPSSSSGAVGPPARLTELLASKSGNLSPLEQQAAELIA
                     KLPAPAPLPGKSLERIGEAIQRPVASKVGFWIGGSAGLVAVVAAGLLFSYGKSRPTAS
                     QQLPLPVIEQLAAPPMSPLPPSAAPQIESTGGIAQVVPGLLPPIEPGRAPSRPAKKHA
                     AAKPPAALPAEATVAAVLPPIEESPLAIESRLIGQALFALRQQHDPKLAIQRLDDYAG
                     RFPQGSLHDEAQAARVDALILLGKQREALAILEKTSFDRLPRGGELTVLRGELRAASG
                     LCKAALSDFAHVEKSRTSSDVAERALYGQGVCRAHLGDLNGAAGDLRAYISRFPGGRF
                     QSAAQATLRSLASSASATP"
     gene            9145..9414
                     /gene="rpsO"
                     /locus_tag="PKL17_16255"
     CDS             9145..9414
                     /gene="rpsO"
                     /locus_tag="PKL17_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNK46337.1"
                     /translation="MSLATQRKKEIVAKFAKHESDTGSPEVQIALLSERISYLTEHFK
                     SHSKDHHSRRGLLKLVGQRRRMLDYLRSSSEERYRHLIDTLGIRK"
     gene            9490..>11050
                     /gene="pnp"
                     /locus_tag="PKL17_16260"
     CDS             9490..>11050
                     /gene="pnp"
                     /locus_tag="PKL17_16260"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNK46338.1"
                     /translation="MYIKESVFLGGKELSIETGKMAKQSDGAVVIRYGDSMVLVTACG
                     AKTAKDGVDFLPLTCEYIEKTYGGGKIPGGYFKREGRPTDAETLVSRLIDRPARPLFP
                     KGYRCDTQIIAFSLSYDRENPTDVLAMTGASAALHLSDIPWDGPFVGVRVIRVDGQFI
                     INPTFQQRDQADLEFVVAVSRDAIVMVEGGADQVSEDVAVDALFFAHREAQPVLDLLE
                     KLRAAIGKPKRVFVPPQKDEALHKKVKELGAARMRAAVTVREKHQRHDQEALVEQQII
                     SELCGEGMIYAGRNKEVAEAVKSLHKKTVRDMVLGEQVRIDGRRTTDIRPITTEVGLL
                     PRVHGSALFTRGETQALVTATLGTSQDEQRLDTLLGDVTKRFMLHYNFPPFSTGEAKP
                     MRQSRREVGHGALAERALLRVMPTEKEFPYVVRVVSETLESNGSSSMAAVCGGSLALL
                     DAGVPIVAPVSGIAMGLIYEKGQDGAADRIAILSDILGDEDHLGDMDFKVCGTRKGIT
                     SVQMDIKIPGLS"
BASE COUNT         2076 a         3291 c         3506 g         2177 t
ORIGIN      
        1 tgatgtcggc gcaggccggc ggggcagggg ttttgcggct gcgggcccac gtcgacattg
       61 cgacgcgacc gccacagcgg ctcgatgtcg attcgcgcca gtttctgaag tttcgttttg
      121 gcgccgccgt cgagtatcgc caattttttt cgcgcgacgc ccgcatcggc tatccgcagc
      181 aagtcaacgc ccagagcgac gccgacctgc gaatcaaaga cggcgatccg ctgtcgtttc
      241 gcctgtataa ccagtttttg gtcacaaacg atgcgcgcaa cctcgaggtc gcgacgacgc
      301 agacctttgc accgcgcatc ttcgagcggc tgagcttgct cgggatgtat cgcccgtttg
      361 gcggcccgct tgaaatcggt ctgtcagaat cgctgcgcgt cgaccactac gtccaaagcg
      421 agctgtcacg cggtcggtcg ttccaaaacg acatcgcact gtggggcgcg tacaagatgc
      481 tgccgcagac gcagctcaag ctggaagtcc gttcgtcgtg gaccagcttt tatggcgatg
      541 gagcggtgat tcccaattcg gcgccgttgc gcatcacggg cgggattcag agtctgctgt
      601 ttccgtggct cggggcgtcg ctctacgtcg gctatggcaa cagcttgcac tatgggcttt
      661 tcgacacgac gcagcttgtc ggcgacacct cgtcgtaccg ctacaacaac ttcgtcggtg
      721 gactggaagc gcggctgttg ctagtgccgc gtatgaacct gcgagccggc tgggcgcgcg
      781 acttctttga ttcgatctac gcgacctatc tgcgcgacga tcggctctac gtcgcgtatg
      841 agcacaaccc ctggcgctcg ctgtggcttt cggcgggctt tgaaacgtat ctgcgggtct
      901 atggtccgct ggttgcgccg tcgacgcttt actatcgggc ctacaaaaac ggggcaacca
      961 cccgcagcga cacgctgttt gcgctcaacg tcgatgccag ctatcggccc ctgtcgtggc
     1021 tgcaagccgg gataagctac aacctgctcg ctgacgtgac cgattttggt tttgtcgatg
     1081 gggctgggac gcggatggat gcgggctttc tcaagcatgt cgtgctgttc aaagccgatt
     1141 tgtcgtactg acggacaggc tgacatgcat tccccacagc acaaaacgca gcgacggggc
     1201 cttttttcct cacgggtctt gcgcaagttt gcgtttgggg tttggctgac tctgggttcg
     1261 ggttgtccgt cgacgcccgc ggttatcgtg cgggaagcga cgccgccgcc gcaagtcgac
     1321 atgagcctgg gccaagatga cgtgtttgaa gttcgcgtct acggagaagc cgagctaacc
     1381 ggcacgtacc gtgtgtcagc cgacggcacg atcgactttc cgctgattgg tcggctcgcg
     1441 gtgcgtgggc agacgacggc gcaggtgtcc gacgcgctga ctgacaagct gcgcgcgtac
     1501 ctgaaacagc cgcaggtgac ggtgttcctc aaggaagtgc acagcaaaaa agtggtgctg
     1561 atcgggcagg tgcgcagtcc cggcacgatc aactttgtcg agggcatgac gctggagcag
     1621 gcgatcagca tggtcggcgg tctgacgccg atggcggcgc gggaccgggt gcgggtgacg
     1681 cgggttttgg gcggcaaggc cgaaacgctg gtggtcaact tcaaagccat cgccgagggc
     1741 gcccagactt tcttccttca gcccggcgat accgtatccg tcgatgagcg cctgttttag
     1801 ttagtaaggc cagccggcgg ggggctcggc gtagcgacga ctaggcacaa cgaacggctc
     1861 ggcgctgcgt cgcaggtaga catcgtgcat gttgtcgccg tcgccatcga cgtccacacc
     1921 cattgcgaca agaccgctgc ggtcgttgta gcgaacgccc aggaccacgg acggcgtcac
     1981 cggattgtgg cgataaaaca cgacttcttc gacgggagag cttacccgct caccaaatcc
     2041 ggtgcccagg ccgggccgct tgtcccgctg catggtttca cgatccgcaa gcgcaccgcc
     2101 accggctcga gcacgctcgt ccatcgggct catcggggcg ctcgtcgcgg gcgctcctcc
     2161 acttggtggc gcagacttgg ctgcgggcgc gcgctcctcg ctgtagtcat ctccaccatg
     2221 accatacagt tcgtgccgcg gaacgtagag cgggcgtggc ggcggcgggg gcgggggcac
     2281 atatcgttcg gggaacacgg cgacgccaat cacgccgact tcgcgagtcg aacccgtccg
     2341 tcccacatac gagttggcca ccgatgaaaa gcggaatgcg gcggcttccc cttgcgacac
     2401 ccgccagccg tcgacatcga catatcccca gggcggaacc aagtagccgc gctttaggcg
     2461 atagtctgca gactttccgt cgacaacatc gcgcccatcg accgacacca ccgcttcgac
     2521 gcggcgaccc gagtgattgt gaatccgcag cgtgtagcgc tgaccgcgct gccccatcac
     2581 ataggtttcg ccgttgtgat agtaggtcgg cgcctgggcc ccgtcggtca tcacggaaag
     2641 ctcgtaagtg ccggcctggt tgcgcagtgg tgtggccgat gccgtggcac acacgacggg
     2701 caaagacagt agcgacagtg caagcagcgt cttcatcttt caaactcctt gtcgggagca
     2761 aacgccgctt gccaaaaaaa gatccggctg gtttgcagtt cttccgtcta ggcgcaaacc
     2821 tctggaagag acagcgcttt tttcagttct tggcacacgc ggtggacatc ggcagcggat
     2881 agctcgctgt gcagtggcag ggccagaccg cgatcgtgca gccgatcgct gaccgccagc
     2941 gccaaaggac cgccggtttc ggaaaaacaa ggcgcggtac gaaacgccgc cagccggtgc
     3001 agtccatacg atgcgatctg ggtttcgatt cccgcctctt tgagcttggc acgcagcgac
     3061 ggacgattca caccttcggg aagcaccacg gcgaatgtct gccagatgcg atcgggatga
     3121 tcggcctgaa accccagccc cgtgtcggcc agattgcgca ggtattgctc ggccagctcg
     3181 cggtgtttct tcacaagctg cggcagcctg cggagctgca cccgaccgat cgccgccgcc
     3241 ggctcggaca gccgatagtt gagcccactt gccggcagtt ccatcacttc ttcacccgga
     3301 acgctggtaa gcccgtgggc gcgcagccgc cgcagcgttt cacagagctg ttcatcgtcg
     3361 gtgagaatcg cgccgccttc cccggtggtc agcagcttgc gcggatggaa cgatagacac
     3421 gccagcttgc cgcccatcgc aggccgcacc gcgatcgcac aggccgcatc cgaaatcagc
     3481 aggtcgtcgt tccgaaccgg cagcggtgac ggatatccga actgatggac gacgagcgct
     3541 gggccacgac gcgagtccag ccaggcctgc acggtttctt ggcgcagaca gaacgtgtca
     3601 gcatcgacat cggccagatg gacctgcggt ggcgtgggca gaaaattggc gacattggcc
     3661 gtcgctggaa acgtcagcgc gggcgacaaa atcgaagtca gcggcagctt ctgcgcaagc
     3721 agacccagat gcagcgccgc cgttcccgag cttacggtca cgcagaaccg cttgccacac
     3781 gcggcggcca gctcttcttc gaacgcgcga ttttcggggc cgtagaccag ccagccggat
     3841 cgcaggactc gctcgctggc tttgatttct tcgtcggtga tgctcggtcg gctgagtgaa
     3901 atggtggtca tgtggggtct tgcacagtcg cttcggaaag cgtgtcaggg tgttcgacgt
     3961 aggctttgag catgtcgagg ctgcgcgcac agtaccagcc gtcggtgatt cgctcgtcga
     4021 aggtgtactt gatggtgacg cccggtcgcg tggtgagtgc gcttgcgtgc ccgtcgacaa
     4081 acggcagatg ggtaagctgt cccaagacgg cgaaaaacga caccgtgccg tactcaaaca
     4141 agtggtgata ggcgctgtcg agcccgatcg agccgaggtt ggcaaaaaac aagctcgcgt
     4201 agagcgggtc cgattcggtc agcgccgatg gcagcaggtt ccacgcatcg aggctgcgct
     4261 gtatccacat gatgaagcgc agaagcggcc ccggcaggcg cgtcaaaatt gccatttcat
     4321 ggtccgcttg gctctttttg tcgcttcgtc cgacggtgac ggcgccgcgc gtctgacttg
     4381 cgatgtcggc caagctttcg cgacctgtga agcgcagctt ggtggtggcg agaacgcctt
     4441 tgtcgctgcg ctctttcttg atcgcaaacg acagctcgat gtgtcgtcgc tggtaaatct
     4501 tgccgccgga aacaaaacgg ttcagttcgg gccgctcgga aaacacccgc gccgtcgctg
     4561 ccagcaaaag atggaagaag ttgatttcgt ctgttttgct gcgcccaagc tgcgcgttga
     4621 gcttggccag gtagggcagc gtctcgctta agtcgagctt ctgttcaaaa tagacgcacg
     4681 actcgttgcg ggtccgcagg atgtacggca tcatccgtcg catcggcgat agtttgtcga
     4741 cgagaattcc atccgggcga cgaaacagca tggcggaaga tgatacgcgc agaaactacg
     4801 acatcaaatc gcgcgttttg ggcagcgtgc ttcggttttg cggcgtgctc gcggtcggct
     4861 gtctgacttc ggcgtgtgcg gtgacgattc cgcaagatgg tctgtgcgct ccgatggcaa
     4921 atcccaccgt cgactgtcag gacggacgcg ggctgggcgc ggtggtggtg cgctggcgga
     4981 tggcggattt gcagcttggg cggctgctcg ggcgcgggct gtgctgctgt aaccccagtc
     5041 cgagccgcga cggcatcgac cgggcgcagt gcgatcaaat tggtagcagc tgtttggatt
     5101 ctcccgcttg gctcgtcgag cacgtcaacc tgactctcca tcgcagcgac gacgatacgg
     5161 aatactcgtg ggacattccg tgctcggagg gggaatttac gacgccgtac tgcattcgtc
     5221 cggggcttta cgacatgaag cttaccgccg acatcaacaa gctggccgac gatggctcgt
     5281 cgcggtttct gtgcgcgcag aaaaaagcgg tatcgccacc tgcggtccgg cgcgcaatcg
     5341 tcgaagggca ggcgaccaac ctggatgcgg tggtgctggg gataaacgcg ccgtagcggt
     5401 cagttgagcc acttgcccgg cggcgtcggc agtgtgtcgg gggctttctc ggcttgttcg
     5461 tcaccgagtt cgagaccctg gcgcgtcagc agaaagtgca cgacgccttc gccggactcg
     5521 gtgctgatct cggcctcgaa cgcgggtccg acgacgctgc ctatcgccag gtctttgtgc
     5581 agtcgcacca ccttgccacg cagcaaatcg ccttgcagtc gttcgctgtt ttccccactg
     5641 cgatgcagcc gataggccgc ttgatacagg cgaagggaaa cctcgggccg cagcggcagc
     5701 gaactggaca aaaccgcgat gagcagccaa tacggcggtg gcggtgaatc ggcagaagcc
     5761 atggatgcag cctaagagca cagagtcgcc ggggcaactc aaggcaaaaa aaaactcgcc
     5821 agtttgtggc gagcttttgg tgaccccagc gggacttgaa cccgcgtttt cggcgtgaga
     5881 ggccgacgtc ctaaccgcta gactatgggg ccgagagact tgggctttct aacaccggcg
     5941 atgcgggaag gcaaggccac agttggtcag aaaagttggt tcggggacta ggattcgaac
     6001 ctagataacc agagtcagag tctggtgtcc tgccgttgga cgatccccga gtgatgccca
     6061 caaaggaacg gggagagaga ttactttttg cggcaaagga gtcaacagtt ttttcgtgat
     6121 ttcgcggagt ttcaccgggc tttgggggac cggctctgcg cgggttcacg gacgctgctt
     6181 gctccgctgc ggcggcgagt gttctttcgt ccgaaccaag tgggggaagg cgcgcgggcg
     6241 cgactacttc ttgatgtggg tcttgatgta gttaatcgcg tcctgaacgg tcttgatctg
     6301 ctcggtgtcc tcgtcgggaa tttcgattcc gaactcttgc tcgagctcaa gcaccagttc
     6361 cacgacggcg agcgagtccg ccttcagatc atcaatgaag ctcgcctcgg gccggacttt
     6421 gtccagcgag acttcgagct ggctacagac gatgtctttg acctttgctt cgatatcagc
     6481 gtgcatgatt cctccacttg tcggcctcgc gtaggggatt gttcagacgg cgcttccgtc
     6541 ggagacaagc cagttccgcg gccacgaatg cgacattttg aacttttgtc ccgacaaacg
     6601 ccagacggaa aatcgcagat agatcggtct tctgacccga ccatcgtgac ttcaaccggc
     6661 gtcgcttcgg gctgcgtgac ctgctcgtgc cgttttaccg cgagccccag gtttttgcag
     6721 ccacctacaa gtcgctgtca cttgcgacgc tgtcatcgct gcactcctct agaccggcga
     6781 gcgcggcggc atcgctcatg tggcggacga ccttccagca ggtggccagc gaaaagcccg
     6841 cccgctggag cgctcgcaaa gcccgcgccg tttctttttg caccttggcg tcttggggcg
     6901 actttctgga cgatgtgctt ccgctagaac ctgggctgct tgtgatgcgg aggcgctttt
     6961 tttctacaaa cgaaagcgca agctgtagct cgtcgacttc cgaataggcg cgctcgacgg
     7021 tctgttcgac cagatcggcg ttgacgccgc gacggcggag ttcctgcgcc acccgacgac
     7081 gacccaggtg tgattgcttc tgccgacttt cgacgaaatt ttcggccaga cgcgcatccg
     7141 acagatatcc cagctcgtgc agacgggcga tcacttggtc gatgcgctgg cgacttggct
     7201 cgtctagctt gcgggtgaga atgcgccgca gctccgcttc gcttcgggat cgggtggcaa
     7261 gagcggacac cgcggcttca aaaagctggg catcggtgag cggtggtcgt tcggtctttc
     7321 gtcgagcgct gcgggcaaac atggcggaaa acctagcatg tgattttccg catcgctcgt
     7381 aaggttttga ccccgccgaa gcctaagata atgccgtgac cgctgcacct tcccttgtga
     7441 ctgcgccgcc cgctgcctac tccgttacgg aggtttatcg gctgcatgct ggcgacgtgg
     7501 ctcgctgggc ggctcgactc ggtgggccgc aagtggacgt cgaggatgtc gtgcaggatg
     7561 tgtttctgac ggtacaccgt ctgctccccg agtttcgcgg cgaagccaag atcacgacct
     7621 ggctgttccg aatcacgcag aaccaagttc gtcatcgctt gcgacggctg cgactgcgtc
     7681 ggctgtttcg cagcagcgat gaagacgacg tgatttcgga gctgccgtcg cacggacctt
     7741 cgccgattga tgacctcgag cgcaaggaag cccgcgccgt ggtctatcga atcctcgacg
     7801 ggatgtcgga aaagtaccgc acggcgttca tcctcttcga gatcgaaaag ctaccgggcg
     7861 aggagatcgc gcagcttctc aatcagaagg tggcaacgat ctgggtctgg ctgcatcgcg
     7921 cccgcgccca gtttctcgaa ggcttagaga agcacaacaa gagcgaacga tcatgaagac
     7981 gacttctcct tatccttctt cctcgtcggg ggcggtgggt ccgccggcgc gactgacgga
     8041 actgttggcg tcgaaaagcg gcaatctgtc gccactcgag caacaagcgg ctgagctgat
     8101 cgcgaagctg ccagcgccag cgccgcttcc gggcaagtcg ctcgaacgca ttggtgaggc
     8161 gatccaaaga ccggttgcgt cgaaggttgg cttttggata ggcggcagcg cgggtcttgt
     8221 cgcggtggtg gcggcgggtc tgctgttttc gtatggcaaa agccgaccga cggcttcgca
     8281 gcaactgccc ctgcctgtga tcgagcagct tgcggctcca cctatgtccc cgctgccgcc
     8341 gtccgctgcg ccccagatcg aatccacggg gggaatcgcc caggtggttc ctgggctgct
     8401 tccgccgatt gaaccggggc gggcgccgag tcgtcctgcc aaaaagcacg cagcagcgaa
     8461 accaccggct gcgctaccag ccgaagcgac ggtagcagcc gtgctgcctc ccatcgagga
     8521 aagcccgctc gcgatcgagt cgcggctgat cggtcaggcg ctgtttgcac tgcggcagca
     8581 gcacgatccg aagctcgcaa tccagcgact ggacgattac gcaggccggt tcccacaggg
     8641 cagcttgcat gacgaagcgc aggccgcacg ggtcgatgcg ctgatcctgc tgggcaagca
     8701 gcgcgaggca ctggcgatct tggaaaagac atcgtttgat cgcctgccgc gcggtggtga
     8761 actgacggtg ctgcggggtg aactgcgcgc tgcgagcggc ctttgcaaag ccgccctttc
     8821 ggactttgcg cacgtcgaga agtctcgcac cagcagcgat gtggcggaac gggcgcttta
     8881 tggtcagggc gtctgccggg cccatctggg cgatctgaat ggggcggctg gcgatctgcg
     8941 cgcttacata agtcgattcc ccggtggccg ctttcaaagt gcggcccaag cgacgctgcg
     9001 cagccttgct tcctccgcca gcgccacccc atagcaagag ccgtcggcgc cttcttgcct
     9061 acctcccgcg aacatgtgtt gacagaccgc ttggtttggg aaaagactgt cccaaaaaaa
     9121 attagaacga attgtggagt cacaatgagc ctcgctacgc agcgcaagaa ggaaatcgtc
     9181 gccaagttcg ccaagcacga gtcggacacg ggttcgcccg aagtccaaat cgctctcctc
     9241 agcgaacgga tttcctacct caccgagcac tttaagagtc acagcaaaga ccaccactcg
     9301 cgacgcggcc tgctcaagct ggtcggccag cgccgtcgga tgctcgatta cctccggagc
     9361 agcagcgaag agcgctaccg tcacttgatc gatacgctgg gcatccgcaa gtagccaccg
     9421 ctgcggaccc caccctgctg accctccacc gccggtccca caaacgggac cggtccatat
     9481 cgagcgaaga tgtacatcaa agaatccgta ttcctgggtg gcaaggaact gtccatcgag
     9541 acaggcaaga tggccaagca gtccgacgga gcggtcgtga tccgctacgg cgacagcatg
     9601 gttctcgtca ctgcctgcgg tgcgaagacg gccaaagatg gcgtcgactt cctgcccctg
     9661 acctgcgagt acattgaaaa gacctacggc ggcggcaaga ttcccggcgg ctacttcaag
     9721 cgcgagggac gcccgaccga tgccgagacc ttggtctcgc ggctgatcga caggccggcc
     9781 cgtccgctgt ttcccaaagg ctatcgctgc gacacgcaga tcatcgcgtt ttcgctgtct
     9841 tacgatcgcg aaaatccgac cgatgtgctg gcgatgactg gtgcgtcggc ggcgctgcac
     9901 ctgtcggaca tcccctggga tggtcctttc gttggtgtgc gagtgattcg cgtcgatggc
     9961 cagttcatca tcaatcccac cttccagcag cgtgatcagg cggatctaga gttcgtcgtc
    10021 gcggtgagcc gtgatgcgat cgtcatggtc gaaggtggtg ctgatcaggt gtccgaagac
    10081 gtggcggtcg acgctctgtt tttcgcacac cgagaagcgc agccggtgct cgaccttctg
    10141 gaaaagctgc gggcggcgat tggcaagccc aagcgcgtgt ttgtgccgcc gcagaaagac
    10201 gaagcgctgc acaagaaggt caaagagctt ggcgctgctc ggatgcgcgc ggcagtgacg
    10261 gtgcgcgaaa agcaccagcg tcacgaccaa gaagcgctcg tcgaacagca gatcatttcc
    10321 gagctgtgcg gcgaaggcat gatctacgcc ggtcgcaaca aggaagtcgc cgaagcggtc
    10381 aagtcgctgc acaaaaagac ggtgcgtgac atggtgctcg gtgagcaggt ccgcatcgac
    10441 gggcgacgga cgaccgacat ccgaccgatc acgacggaag tggggcttct gccgcgcgtc
    10501 cacgggtcgg cgctgtttac ccgcggtgaa acgcaggcgc tggtgacggc gacgcttggt
    10561 acaagccaag atgagcagcg gctcgatacg ctgctcggcg acgtgaccaa gcgcttcatg
    10621 ctgcactaca acttcccgcc gttttcgacc ggcgaagcga agccgatgcg gcagtctcga
    10681 cgagaagtcg gacacggtgc actggccgag cgggcgctcc tgcgggtgat gccgacggaa
    10741 aaagagttcc cgtatgtggt ccgggttgtc tccgagacgc tcgagtcaaa cggtagctcg
    10801 tcgatggcgg cagtctgcgg cggttcgctg gcgcttttgg atgcgggcgt gccgatcgtt
    10861 gcgccggtgt cgggcatcgc gatgggtctg atttacgaaa aaggccagga cggcgctgcc
    10921 gatcgcattg cgattctgtc ggacatcctt ggtgatgaag accacctggg cgacatggac
    10981 ttcaaggtct gtggtacgcg caagggcatc accagcgtcc agatggacat caagattccg
    11041 ggtctgtcgc
//
LOCUS       DAORGN010000169        10950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2525_length_10950_cov_5.462414, whole genome shotgun sequence.
ACCESSION   DAORGN010000169 DAORGN010000000
VERSION     DAORGN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_10950_cov_5.462414"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4172
                     /locus_tag="PKL17_16265"
     CDS             <1..4172
                     /locus_tag="PKL17_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46339.1"
                     /translation="DIKPHNTICSDDGTVKLLDFGLAKLRDGSHPSEAAPLFALPTSA
                     SRLAATAPPDDFEPQQSPLLESDSIPGDAVDTEKLALQNTVRSASPNPQPRNGGDAQC
                     LTQEGAVLGTPLYMAPEVFRGEPATERSDIYSLGALLYELATGRPPHVADTLVELELQ
                     VASEEPAKVRELAPLLDVRLGEIIDRCLSTSPAARFASVDELCAALERTGGPPSVRRS
                     DNPFLGLRSFDIADSGVFFGRASEVSELHARLRHEAMLLVAGDSGVGKSSLCRAGVAA
                     SVLAFGMDDGRQYQLVEFAPGHTPGRALCDALSPLLGQPASELYATLRSDPGTLARGL
                     QRHPGDAGGVLLLIDPLEELCTQSDPAEAELLSEFLGLLSVKAPGLRVLLCARGDFLT
                     RLSALPGLGTDFSSRLYIVRPLSEEGLRQAICEPAAQLGVHFETPALVDELLRSTLHT
                     DGGLPLLQFALAELWEVRDKQSGLITADALSSIGGVAGALARHADQVLRALLPTERLA
                     AKRILLRLLTPDGLRATKTLNDIRTSDPAEPIALEALVRGRLLLAREVGGETAYELAH
                     EALLKGWAELRHWLDSEGERRLVVERLSQAASEWQRLGQLQELLWDERRLLEVELLSV
                     ESHMLTELSQRFLAESQRNSKRRARTRRGIVAVLLLLPLLIVLLSWQVHTATEQRRLR
                     RAAEQSELGMRAITLSQLPGSELLALQAALEATQSTDGTLPVSQAIEGLFVALHAAQR
                     SLPLKSPPAQEISISPTGDWTVIATSDGPLLSFARGQRRVAQTLSVQSRARQLRWIDG
                     SRLVAGYDDGSLRLLDVPTGKTLKLIAAHRDRITDLAVFDDGKQVLSASLDGSAALWD
                     LSQGKLTRRFDIDGQPVRSATLFGPHVFLATASGELRVVKHSDGSIEKNLSCDDGTRR
                     SDGGQITSAAHRKDGIISIVSGGRQQVCLVDGTSLQLVSTLRIDGQAIFACFQGPAGL
                     VLLGQQGTIRIIDPQALTVRSEQKLPSSLRAAAMGPAADRLVLGSSDGTISSLQIESG
                     RITTLADGAVDPVTCLASSDGITVSQTSDGSIRAWQPDSDGAALWKKRLSEPALKMLR
                     VPGQRAILLLGQSGKLSLLDETQTDITTLSKDAVLSLSGDGKTLMIGHPDGQLELFSL
                     DSRRTIHKWRGHQAAVTEVTFSIDGQFLASSSRDLTAALWRTQDRALVERFVEPNPAT
                     GVALGQSGSLVVTANSIGRVALWERPSTGPLLRLRPSDDDPFATHLVRSAGGTQLLVL
                     GAQTVLSPLAPTVSAPLRLQGHLGPTLRGSFSASGHEVVTLGKDRSVRVYDAQFGTQK
                     LILRPDDPRDVLFGENPSQIIVASHRTQSLIGYPASLDKLRKQACALLAPGSDEFERS
                     DLCAESEASGP"
     gene            complement(4169..4951)
                     /locus_tag="PKL17_16270"
     CDS             complement(4169..4951)
                     /locus_tag="PKL17_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46340.1"
                     /translation="MFATSLPLRKLALSPAHRAVEKLYLAFSPVWMAIIAYCMLTHCF
                     ATWGDVGHLGLGLGLSLPLWLIPLFLPSESEQGIPFLQRHSTKSNLFVALMSFVQCYF
                     GSAMFFEGLGMQYHFPTTWSLSGTPVFLYLVTVAYFSTYYALMRLGLRWFLQVVPSPG
                     RTLVWLVIAALSYSMAFGETFFMASPSLAAFFSYADRHKALLIGSLCYGTLFFISLPV
                     FLRLDEDPRVPTPMSRLFWQTMGANMLILCAYELYKVLLPRV"
     gene            complement(4992..6518)
                     /locus_tag="PKL17_16275"
     CDS             complement(4992..6518)
                     /locus_tag="PKL17_16275"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46341.1"
                     /translation="MAEIFLARPAPGQGGQGVRPGPFVVKRLFAHQSADTEFVRMFKN
                     EARLAALLKHPNIVDIFDQGEIDGSFFLAMEFINGEDLRSIAQQSDTMNRRPPLPVVG
                     RIIMDMLAGLHYAHSLADENGKPLGVVHRDVSPQNILVTYDGQVKIIDFGIAKATMAA
                     ESEQTQAGMVKGKYAYMSPEQARSAKLDGRSDVFSVGILLWELITWRRLFKRGTDLAT
                     MVAVAEEPAPSMLLLTPDCPQALDDVVQKALAKDTEQRYRSAQEFAAALGDCMTKLGW
                     DFAPKAVGAYMSEIFRERVAQRESELRAVMNAAKRDSLRQAVPAIQLPPSQPTPKAPS
                     VTMPLQVINLQQGPGGRPVVPANLRTTMPFGPSGLQAARPGARPAAPAGLTPPSAKEA
                     SSDVLALRKNPSPAASPVAPSSNEPDTVPMRKATPPGGTPPVAGPTAAPGAKRSPADG
                     IKVDAATNASLATVPTPALNRSRSKENLPAHRQRVILAIALGVSIGVLAVIVLQLLLR
                     "
     gene            6726..7889
                     /locus_tag="PKL17_16280"
     CDS             6726..7889
                     /locus_tag="PKL17_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK46342.1"
                     /translation="MYDLNFTEEQLQLRKTARDFARSKIAPVAGQLDEEGHFPAELLK
                     EGWNLGLMNCEVPEEFGGLGLSCLSHCMILEEIAWACAGVNTSMAANALGSLPIMIAG
                     TREQKEKYLGWLTREPIFAAYCCSEPDAGSDVAGMRTHFVKDGDSYVLTGQKRWITNG
                     GHASFYTVLATSDPKPEKDLKVRNRKIAAFVVDRDLPGVKVGKKENKMGQRASNTTDV
                     IFDEVRLPKEALLGQEGEGFKIAMKTFDRSRPWIAAGAAGLIGRALHESRAYALERKT
                     FGVPIAAHQAIQFILADMAVALEATRSLCHRAAWMVDQGKIDSIVSSMAKLYGADMAM
                     KATTDAVQVFGGYGYTKEYPVEKLMRDAKLLQIYEGTSQIQRMVIAKNVLTGE"
     gene            8107..8613
                     /locus_tag="PKL17_16285"
     CDS             8107..8613
                     /locus_tag="PKL17_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46343.1"
                     /translation="MLRRGFRNSFPPRASGGPPTRISVTDLPSFLDDRQQSDVCIGVP
                     SNQGSLSRVLLRQRLTMLAAIDHAGQLAERLAEEHDETVLSPSLQPSSVHVVPHPDGC
                     QVWLDYDLSARTRNRTQAMVMRRLTLLVWVRQDSASMSSPNPLLTRDAAPDHAPIDSS
                     SAGHGHDD"
     gene            8723..10231
                     /locus_tag="PKL17_16290"
     CDS             8723..10231
                     /locus_tag="PKL17_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK46344.1"
                     /translation="MSAPSEPRRKLPDLLPPTKVHYEGARPQTIQLRTCRLITGHGPS
                     RREHLFDQTTIRIGAMEDNDLIVSDDTVSRFHCRIEQDEVGYILRDLGSTNGTYINGI
                     RVREAYLHPGVVISIGQSDVQFFVGQERVEVIPSSKNRLGDIIGKNVKMRELYAIIEK
                     IAPTGATVIIEGETGTGKEVVAQTIHKLSPRAPQSMMVFDCGAVPKNLIESELFGHEK
                     GSFTGAIMTRQGLFEMAHNSTLFIDELGELPMELQPKLLRALEQREIRRVGATRSLKV
                     DVRIIAATNRQLEEEVRAGRFRQDLYYRLSVVRLLLPPLRERIDDIPLLVESFLGEHS
                     YNRLPDGSRKCTHISSDAMELLVRYRWPGNIRELVNAIERAVSFASGSEIRPEDLPEN
                     IRVGDSHGFLPEPSTRPAESRKSGEVPRPVTDTPSGGTGHEPRLDLPFKDAKDQWIAQ
                     FEQKYVAFLLKKHKGNISHAARTADIDRKYFRKLMRKYDLDSGKDEPIDDDE"
     gene            complement(10273..>10950)
                     /locus_tag="PKL17_16295"
     CDS             complement(10273..>10950)
                     /locus_tag="PKL17_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HNK46345.1"
                     /translation="VLLSETDFSSIVKKIQASKADVVLNFVQGDSNLPLFRELRAAGV
                     TPAKVPVISFGIGENEISQLDGVDLTGDYLAWSYFESVDRPQNKQFVEAFKKKFGDHR
                     GISDPMEAAYAGVYLWARAVKAAGSDDVRAIRKALQGQSIEGPGATLRLDPGNNHAWK
                     MFRMGKLVGGNHIEVVYGSDKLLSPDPFPGTRTRAEWEALLQFLHSQWKGNWVNTDRP
                     NLLKRGK"
BASE COUNT         2247 a         3483 c         3252 g         1968 t
ORIGIN      
        1 gcgacatcaa accgcacaac accatttgca gcgacgatgg aacggtcaag ctgctcgact
       61 ttgggttggc caagctgcgc gacggaagtc atcccagcga agcagccccg ctttttgcac
      121 tgcccacgtc cgcatcgcga ctcgcggcga cagcgccccc ggacgacttt gagccgcagc
      181 aatccccact tctggaatcc gattcgattc ccggcgacgc cgtcgacacc gaaaagctcg
      241 cactgcaaaa caccgtgcgc agcgccagtc cgaatccaca gccacgaaac ggcggtgacg
      301 cgcagtgttt gacccaagaa ggcgcggtgc tcggcacgcc gctgtatatg gcccccgaag
      361 tctttcgcgg tgagccagcg acagagcgat cggacattta ttcgctcggg gcacttctgt
      421 acgaacttgc gacgggtcgg cccccgcatg tggcagatac cctcgtcgag ctggagctac
      481 aagtcgcatc cgaagaaccc gccaaagtcc gtgagcttgc accgcttctc gatgtccggc
      541 tcggtgaaat catcgatcgc tgtctgtcca caagtccggc agcacgcttt gcatccgtcg
      601 acgagctgtg cgcggcgctc gaacgaaccg gcggcccccc cagcgtccgg cgcagcgaca
      661 atccgttcct tggcctgcga tcgtttgaca tcgccgacag cggcgtgttt tttggccgcg
      721 ccagcgaggt cagcgaactt cacgccaggc tgcgccacga agcgatgctg ctcgttgctg
      781 gggattcggg ggtcggcaag tcgtcgctct gccgggccgg tgtcgcagcg tcggtgctgg
      841 catttggaat ggacgatggt cgccagtatc agctggtgga atttgcgccc ggacatacgc
      901 cgggacgagc actatgcgac gcgctgtcgc cgctgctagg ccagcccgca agtgagctgt
      961 atgcgacgct gcgcagcgat ccggggacgc tcgcgcgtgg gcttcagcga catcctggcg
     1021 acgcaggcgg tgtccttttg ctgatcgatc cgctcgagga gctttgcacg caaagcgatc
     1081 cggcggaggc cgagcttctg tcggagtttc ttgggctgct ttccgtcaag gcgccgggct
     1141 tgcgggtgct tttgtgcgcg cgcggtgact ttctgacccg gctttcggcc ctgcccggac
     1201 tcgggactga tttttcatcg cggctgtaca tcgtgcgtcc gctgtcggaa gaaggcctgc
     1261 ggcaagccat ctgcgaacca gcggcccagc tcggggtgca ttttgaaaca ccagcgctcg
     1321 tcgatgaact gcttcgctcg acgctgcata ccgacggggg actgccgctg ctccagtttg
     1381 cgctcgctga gctgtgggaa gttcgcgaca aacagtcggg gctcatcacc gccgatgcac
     1441 tttcgtcgat tggtggtgtc gcgggcgcac tggcgcggca tgccgatcag gtgctccgcg
     1501 cgcttttgcc gacggaacgg ctggcagcca aacgaatcct tctgcgactt ttgacgccgg
     1561 acggcctgcg cgccaccaaa acgctgaacg acatccgcac aagcgatcct gccgagccca
     1621 tcgcactgga ggcactggtt cggggacggc ttttgctcgc gcgggaagtg gggggcgaaa
     1681 cggcttacga actggcgcac gaagccctgc taaaaggctg ggccgagctg cgccactggc
     1741 tcgatagcga gggggaacgc cgactggtcg tcgaaaggct gagccaagcg gcgtcggaat
     1801 ggcagcgcct gggacagctt caggaactgc tgtgggacga acggcggctg ctcgaagtgg
     1861 agctattgtc cgtcgagtcg cacatgctga cggaactttc gcagcgcttt ttggccgaat
     1921 cgcagcgaaa cagcaaacgt cgcgcgcgca ccaggcgggg gatcgtcgct gtccttttgc
     1981 tcttgccgct gctcatcgta ctgctgagct ggcaggtgca tacagcgacg gaacagcgac
     2041 gattgaggag agccgccgaa cagtccgaac tgggcatgcg tgcaatcacg ctgtcgcagc
     2101 ttcctggcag tgaactgttg gcgctgcaag ctgccctgga agccacgcag agcaccgacg
     2161 gaaccctacc ggtctcgcag gcaatcgaag ggctgtttgt ggcactgcac gcggcgcagc
     2221 gatccctgcc gctgaagtcc ccccccgcgc aagaaatcag catttcaccg acgggagact
     2281 ggacggtgat cgcgacttca gacgggccgc tcctttcctt tgcgcgcggt cagcggcgcg
     2341 tggcgcagac gctgtctgtg cagagccgag cccgccagct tcgctggatc gacggatcga
     2401 gacttgtggc aggttacgat gacggttcgc tgcgactgct cgatgttccg acgggaaaaa
     2461 ccctgaagct tatcgccgcg caccgcgatc gaatcaccga tctggctgtc ttcgatgacg
     2521 gaaagcaggt gcttagcgcc agtctcgacg gcagcgctgc gctgtgggat ctgtcgcaag
     2581 gtaagctcac acggcgtttt gacatcgacg gacagcctgt tcgatcggcc acacttttcg
     2641 gtccgcacgt ttttttggcg acagccagcg gtgagctgcg cgtggtcaaa cacagcgacg
     2701 gcagcatcga aaaaaacctg agctgtgacg acggaacgcg acgcagcgat gggggacaga
     2761 tcacaagtgc tgcgcaccgc aaagacggca tcatcagcat cgtatccggc ggtcgccagc
     2821 aagtctgtct tgtcgacggc acgtccctcc aattggtgag cacgctccga atcgacggcc
     2881 aggcgatctt tgcgtgtttc caaggccccg ccggactcgt tctcctcggg cagcagggaa
     2941 ccatccgcat catcgatccg caggctctca cggtgcgcag tgaacaaaaa ctgccaagca
     3001 gtctgcgcgc cgcagcgatg ggaccagccg ccgatcgcct ggttctcggc agcagcgacg
     3061 gaaccatcag cagtctacag atcgaaagcg gcaggatcac gacgctcgcc gacggggcag
     3121 ttgatccggt gacctgtctc gccagcagcg acggcatcac cgtcagtcaa acgagcgacg
     3181 gcagcatccg cgcctggcag cccgattccg acggtgccgc gctgtggaaa aaacgactca
     3241 gcgaacccgc cctcaaaatg ctccgtgtgc cgggccagcg cgcgatcttg ctgctcggtc
     3301 aaagtggcaa gctctcgctg ctcgacgaaa cacagacgga catcacgacg ctgagcaaag
     3361 acgcggtgct ttcgctgtct ggcgacggca agacgctgat gatcgggcac ccagacggac
     3421 agcttgagct gttttctctg gactctcgac ggacgatcca caagtggcgc ggccatcaag
     3481 cagccgtcac cgaggtgact ttttctatcg acggtcagtt cctggccagc agcagtcgcg
     3541 atctgactgc ggcgctgtgg cgcacgcagg atcgggcgct cgtcgagcgc ttcgtcgaac
     3601 cgaatccagc aaccggcgtc gcgctcgggc agtcgggcag cttggtggtg acggcgaact
     3661 ccatcgggcg ggtggcgctg tgggaacgtc cgtcgaccgg ccctcttttg cgcctgcgtc
     3721 ccagtgacga tgatccgttt gcgacccatc ttgtgcgatc tgccggcggt acccagctgc
     3781 ttgtgctggg cgcccagacg gtgctgtccc cgctggcccc cacggtgagc gcgccgctgc
     3841 gtctacaagg tcacttgggt ccgacgctgc gcggcagctt ttccgcatct ggtcacgaag
     3901 tcgtcacgct cggcaaagat cgttcggtgc gggtctatga tgcgcagttt ggcacccaaa
     3961 aactgatcct gcgccccgac gatccgcgcg acgtgctgtt tggcgaaaat cccagccaga
     4021 tcatcgtcgc aagccaccga acgcagagcc tgattgggta tcccgcgtcg ctggacaagc
     4081 tgcggaagca agcctgcgcg ctgcttgccc ccggcagtga tgaatttgaa cgcagcgacc
     4141 tgtgcgccga aagcgaagcg agcggaccct aaacccgcgg cagcagcact ttgtacagct
     4201 cgtaggcaca caaaatgagc atgttggcgc ccatcgtctg ccaaaaaaga cgcgacatcg
     4261 gcgtcggtac gcgcgggtct tcatccagac gcaaaaacac cggcaggctg atgaaaaaca
     4321 gcgtcccgta acacagcgaa ccaatcagca gcgctttgtg ccgatcggca tacgaaaaaa
     4381 acgcagccag cgacgggctc gccataaaaa acgtttcacc gaacgccatg ctgtacgaaa
     4441 gcgcggcaat cacaagccag accaaagtcc gacccggcga cggcacgacc tgcaaaaacc
     4501 atcgcagccc gagccgcatc agcgcgtaat acgtcgaaaa gtacgcgacc gtcaccagat
     4561 acaggaacac gggcgttccg ctgaggctcc acgtcgtcgg gaagtgatac tgcattccca
     4621 gaccttcgaa aaacatcgcc gagccgaaat agcactgcac aaagctcatc agcgcgacaa
     4681 acaggttcga cttggtgctg tgccgctgca aaaatgggat tccttgctcg ctttccgacg
     4741 gcagaaaaag cggaatcagc cacagcggca aagacagccc aagcccgagc ccaaggtgcc
     4801 ccacatcgcc ccatgtcgca aagcagtggg tcagcatgca ataggcgatg atcgccatcc
     4861 acaccgggga aaacgccagg tagagctttt cgacagcgcg atgggcaggg gacagcgcga
     4921 gcttccgaag cggcagcgag gtcgcaaaca tggccagacc ataccgcgac gcgggcgcgg
     4981 ctgggcaagc cctagcgaag caaaagctgg agcacgatca ccgccagcac accgatcgaa
     5041 accccgagcg caatcgccag gatcacgcgc tgacgatggg ctggcaggtt ttctttcgat
     5101 cgcgaccggt taagagccgg tgtcggcact gtcgccagcg aagcattggt ggccgcatcg
     5161 accttgatgc cgtctgccgg actgcgtttg gcaccaggag cggctgtcgg acccgcaacc
     5221 ggaggcgttc caccgggcgg cgtcgctttg cgcatcggca cagtgtccgg ttcattggac
     5281 gacggggcga cgggcgaggc agccggcgac ggatttttgc gcagcgccag cacatccgac
     5341 gaagcttctt tggccgaagg cggggtcagt ccggcgggcg cggctggcct tgctccgggt
     5401 cgcgcggctt gcagccccga cggaccaaac ggcatcgtcg tccgcaagtt tgccggcacc
     5461 accggtctgc cgcccggacc ttgctgcaaa ttgatcactt gcagcggcat cgtcaccgac
     5521 ggcgccttcg gagtcggctg actgggagga agctggatcg ccggtacggc ctgacgcagc
     5581 gaatcacgct tggccgcatt catcaccgcg cgcagctctg attcccgctg cgccacccgc
     5641 tcgcggaaaa tttcggacat atacgcaccg acggctttgg gcgcaaaatc ccagcccagc
     5701 ttggtcatgc agtcgccgag cgccgccgca aactcttgcg ccgatcgata gcgctgctcg
     5761 gtgtctttcg cgagcgcttt ttgcaccaca tcgtcgagcg cttgcggaca atcgggcgtc
     5821 aagagcagca tcgacggcgc cggctcctcg gccacagcaa ccatcgtcgc cagatccgtg
     5881 ccgcgcttaa acaaacgccg ccacgtaatc agttcccaca gcaggattcc gaccgaaaaa
     5941 acatccgatc gcccatcgag cttggccgat cgcgcttgct ccggcgacat gtaggcgtac
     6001 ttgcctttga ccatgccggc ttgcgtctgt tcgctttcgg cggccatcgt cgctttggcg
     6061 ataccaaagt cgataatttt tacctgcccg tcgtaagtga ccaggatgtt ctgcggcgac
     6121 acgtcgcgat gaaccacgcc aagcggcttg ccattttcgt cggccaagct atgcgcgtag
     6181 tgcaggccag ccagcatgtc catgatgatc cgcccgacga ccggcagcgg aggccgacgg
     6241 ttcatcgtat cggactgctg ggcaatgctg cgcaagtcct cgccgttaat aaactccatc
     6301 gcgaggaaga acgacccgtc gatttcgccc tgatcgaaaa tgtcgacgat gttcggatgc
     6361 ttcagaagcg ccgccagccg ggcttcgttc ttgaacatcc gaacgaactc cgtgtcggcg
     6421 ctttggtgcg caaacagccg cttgacgacg aagggaccag gacgcacacc ctggccgcct
     6481 tggccgggcg caggtcgggc caagaaaatc tcggccatgc cgccttcagc cagcttgcgg
     6541 tcgagcaggt atttgccgta gggaagagcc acggactaag aatcctcaga atcgtaggcc
     6601 ggttctttgt ccgcggtcaa cctgatacat tggcgcaaac gcaaaccatg tcctggtctg
     6661 cacggcacac acatcgccac gatgatgccg cgcggccacc gattttcgag aggagacccc
     6721 cacagatgta cgatctgaac tttaccgaag aacagctcca gcttcgcaag acggcgcgcg
     6781 actttgcccg cagcaagatc gctcccgttg ccggtcagct tgatgaagaa ggccattttc
     6841 cggcggagct tctcaaagaa ggctggaacc tgggcctgat gaactgcgaa gtgcccgaag
     6901 aattcggcgg tctcggcctg tcgtgcctgt cccactgcat gatcttagag gaaattgcct
     6961 gggcctgtgc cggcgtcaac acctcgatgg ccgccaacgc gctcggatcg ttgcccatca
     7021 tgatcgccgg cacgcgcgag cagaaggaaa agtacctcgg ttggctcacg cgggagccca
     7081 tcttcgccgc ctactgctgc tccgagcccg acgccgggtc cgacgttgcg ggcatgcgca
     7141 cccactttgt caaagatggc gacagctacg tcctgaccgg ccaaaagcgc tggatcacaa
     7201 acggtggcca cgccagcttt tacaccgtgc ttgcgacgag cgaccccaaa ccggaaaaag
     7261 acctcaaggt gagaaaccgc aagattgccg cctttgtcgt cgaccgcgac ctccccggcg
     7321 tcaaagttgg caaaaaagaa aacaagatgg gccagcgcgc cagcaacacc accgacgtga
     7381 ttttcgacga agtgcggttg cccaaagaag cgctgctcgg ccaagagggg gaaggcttca
     7441 aaattgcgat gaagaccttc gaccgctcgc gtccgtggat cgccgcgggg gccgctggac
     7501 tgattggccg cgcgctgcat gaaagccgcg cctacgcgct ggagcgcaag acgttcgggg
     7561 tgcccatcgc ggcgcaccag gcgatccagt tcattctcgc ggacatggcg gtggcgctgg
     7621 aagcgacccg ttcgctgtgt catcgcgcgg cgtggatggt cgaccagggc aagatcgatt
     7681 cgatcgtctc gtcgatggcc aagctctacg gggcggacat ggcgatgaag gcgacgaccg
     7741 acgcggtcca ggtcttcggc ggctacggct acaccaagga ataccccgtc gaaaagctga
     7801 tgcgtgacgc caagctgctg caaatctacg aaggcacgtc gcagattcag cgcatggtca
     7861 tcgccaagaa cgtccttacc ggcgaatagc ccgttccgct ggagacagcc gaccccaggc
     7921 tggcgtcttt ccacccaagc cgatcgttca caactcgact tgcgtccggc ccaccggact
     7981 ttgaccgccc atcggttccg catacttggc ggcatggccc aagtttccga cggacattgc
     8041 gcgctgtcat gactgcacaa cggatggcac aggctgtgca acggctgatc cgaaaaggga
     8101 caactgttgc tacgtcgagg tttccgaaat agttttccgc cccgcgccag cggtggacca
     8161 ccgacgcgaa tctcggtcac ggacctgccg agctttctcg atgatcgcca gcagagcgat
     8221 gtctgcattg gcgttccatc gaaccaaggc agcctgtcgc gcgtgctttt gcggcaacgg
     8281 ctgacgatgc tggcggcgat tgatcacgcc gggcagcttg ccgaacggct cgccgaagaa
     8341 catgacgaaa cggtgctttc cccgtcgctg caaccatcgt ctgtgcatgt cgtcccgcat
     8401 cccgacggct gtcaagtgtg gctcgactat gacttgagcg cgcggacccg caaccgaacc
     8461 caggcgatgg tgatgcgccg actgacgctg ctggtttggg tgcgccagga ttcagccagc
     8521 atgtcgtcac caaacccgct tttgacccga gacgccgcgc ccgatcatgc gcccatcgat
     8581 tcatcgtcag caggccacgg ccacgacgac tgaacagcca ctgcgctagc ccatcccgcg
     8641 gctgtcatcc cagtgaaccg tgcgctggca ggctaacccc ctgtccttga ctgtaatttt
     8701 tattacatgt ttaatagcca caatgagcgc gccgtctgag ccacgacgga aattaccgga
     8761 cctactgcct ccaaccaaag tccactacga aggagcgcgc ccacagacga ttcagttgcg
     8821 cacctgtcga ctgattactg gtcacggccc cagccgtcgg gagcacctgt tcgatcaaac
     8881 caccatccgc atcggcgcga tggaagacaa cgacttaatc gtaagtgacg atacagtttc
     8941 gcgattccac tgccggattg aacaggacga agttggatac atcctgcgcg acctcggatc
     9001 gaccaacggc acatatataa acggcatccg ggttcgcgag gcatacctgc atccaggcgt
     9061 cgtcatttcc atcggccaaa gcgacgtcca gttcttcgtc ggccaagagc gcgtcgaagt
     9121 catcccctca tcgaaaaatc ggctcggcga catcatcggc aaaaacgtca agatgcgtga
     9181 gctgtacgcg atcatcgaaa aaatcgcgcc gacgggagcc acggtcatca tcgagggaga
     9241 aaccggcacc ggcaaagaag tcgtcgcgca gaccatccac aagctgtcgc cgcgcgcgcc
     9301 gcagtcgatg atggtgttcg actgtggtgc ggtgccgaaa aacctcatcg agtccgagct
     9361 gtttggtcac gaaaaaggca gcttcaccgg cgccatcatg acccggcagg gactgttcga
     9421 aatggcgcac aactcgacgc tgttcattga cgagctgggc gagctgccga tggagcttca
     9481 gccgaagctt ttgcgcgcgc tggagcagcg agaaattcgt cgcgtcgggg caacccgatc
     9541 cctcaaagtg gacgtgcgaa tcatcgccgc gaccaatcga cagcttgaag aggaagtgcg
     9601 cgccggtcgg tttcgtcagg atctctacta tcgcttgtcg gtggttcggc tgcttttgcc
     9661 gcctctgcgc gaacgcatcg acgacattcc gctgctcgtc gagtcatttc ttggcgaaca
     9721 cagctacaat cggctccccg acggcagccg caagtgcacg cacatttcgt cagacgcgat
     9781 ggagctgctc gtccgttatc gctggcccgg caacatccgc gagctggtca acgccatcga
     9841 acgcgccgtc agcttcgcgt caggcagtga aatccgcccc gaggatctcc ccgaaaacat
     9901 ccgcgtcggc gacagccatg gctttttgcc cgaaccgagc actcgccccg ccgagtcgcg
     9961 caaaagcggt gaagtccccc gcccagtgac ggatacacca tcaggaggca ccggccacga
    10021 gccgcgcctc gacctgccct tcaaagatgc caaggaccag tggattgcgc agtttgagca
    10081 aaagtacgtc gcgtttttgc tcaaaaagca caaaggcaac atctcgcacg ccgcacgcac
    10141 cgctgacatc gaccgcaaat acttccgcaa gctgatgcga aaatacgacc tcgacagcgg
    10201 caaagacgaa ccgatcgacg acgacgaata gtctgaccgg tcaagcgaat cgtccgcgca
    10261 aaaaacccga agctacttcc cgcgcttgag caggttcggc ctgtcggtgt tgacccagtt
    10321 tcctttccac tgactgtgca gaaactggag cagcgcttcc cattccgccc gcgtgcgcgt
    10381 ccccggaaac ggatcgggcg acaacagctt gtcggaacca tagacgacct caatatggtt
    10441 tccgccgacg agcttgccca tccggaacat tttccatgcg tggttgttgc cgggatcaag
    10501 tcgcagcgtc gcgccagggc cttcgatgct ttgaccttgc agggccttgc gaatcgcgcg
    10561 cacgtcgtcg gaccccgccg ctttgaccgc cctcgcccac aaatacacgc ccgcatacgc
    10621 cgcttccatc ggatcgctga tgccgcggtg gtcgccgaat tttttcttga aggcctcaac
    10681 gaactgtttg ttttgcggac gatcgaccga ctcgaaatag ctccacgcca agtagtcgcc
    10741 cgtcaggtcc acaccgtcga gctgcgagat ctcattttcg ccgatgccaa acgaaatcac
    10801 cggcactttg gctggtgtga cgcccgccgc gcgcagttca cgaaacagcg gcaagttcga
    10861 atcgccttgc acgaagttga ggacgacgtc cgctttcgaa gcctggatct tcttcacgat
    10921 cgacgaaaag tccgtctccg acagaagcac
//
LOCUS       DAORGN010000170        10944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2529_length_10944_cov_5.742217, whole genome shotgun sequence.
ACCESSION   DAORGN010000170 DAORGN010000000
VERSION     DAORGN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_10944_cov_5.742217"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="PKL17_16300"
     CDS             <1..1866
                     /locus_tag="PKL17_16300"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNK46346.1"
                     /translation="SVVGRDPEAMMETRLLIEAKRPSADLVRVSPGVFGLRVYPPRGE
                     RGEALKAEGEKVTKPKAESAATPAKDPRAGKRRRTGRGAESAEAPAEAAKKPAETAEA
                     VATPAAVEAVSVPVTSAAVDPPASEAKPAPAKGRGRTRSRATETRKAPPATEAPSAKS
                     SPAVAPDIAVAPVAPVVPTVPSQNTPIPAVAAVPSRQTPVPAAAPAVPVPNLTPAVPI
                     IPTTDIAKAAEPSAKTPSPAPTAAPAPVVAVVTEPPASDLAKRPFQSTKSPVPLAPSV
                     PATAESVAATAPVVRPAPSAVTPPRPVEAAAPPRPVEAATPPRNEASGHRPAPPPHAG
                     DRPAAPRDERPAPSANTPAPAQPGPSQPQQPRVMSMADAAYDVLRGASDGRALTNRQI
                     AEIAVKRRLVRGELADVSRSVRVALVREQRHRDSEGLRPRIRTVGPGQYTLGERKLEP
                     ELYNAERELLDRAGRTREATRVALRRRIRQLPSGAFELLMRLLLERMGMVGAEIVKRG
                     DGVAYYGGTLSRGARSIKVLVAVRPGEGELSKEAVGELRAGVRLRGFDEGMILCSARA
                     GAAAHAEASATPGVELYDQDALTELLVRQHLGVRRMHLPVDYLDTELFNELLEQG"
     gene            complement(1921..2241)
                     /locus_tag="PKL17_16305"
     CDS             complement(1921..2241)
                     /locus_tag="PKL17_16305"
                     /inference="COORDINATES: protein motif:HMM:NF017315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HNK46347.1"
                     /translation="MPPQGRLGDKAQVPADGHGCVACVHSCVGPAIAGSPNVNVNSMP
                     ALRVQDPGVHAACCGPNTWNAAMGSGTVFINGKAAHRLGDMTRHCGGVGKLIEGSGNV
                     IVGG"
     gene            complement(2253..3092)
                     /locus_tag="PKL17_16310"
     CDS             complement(2253..3092)
                     /locus_tag="PKL17_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNK46348.1"
                     /translation="MFKTDRDTKAGQVSGGNANTMTVGIVTDNVHDGGDYRVKVKFPT
                     LPASQGSGQQEESFWCRIGTFGAGSGGRGAFFLPEKDDEVMVAFINGDFNQGVVMGSL
                     WNGMDKPSFANKDAKSPTGRYNKDHADFRGQADAKKNDIRAFSSRCGHELIFNDNASN
                     PRVTLQSSTKHRIVLNDAGNEATKIEIYDGKEENYVLIDTKNKKITIESKTGDILIKA
                     KEKVRIEAKNIETESSQNTKCHAGSNYSIKADSNVEVKANGTMTLQSSGTNTIKGSTV
                     NIN"
     assembly_gap    3274..3373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3374..3840)
                     /locus_tag="PKL17_16315"
     CDS             complement(<3374..3840)
                     /locus_tag="PKL17_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46349.1"
                     /translation="MPAPQASVMQNLAKAKFISFATKLPTDWKQPQGEAGKQYTRAFK
                     SSELVGIPPAPPMFVPATVNKYHTDTAKKIGDQFATYIDGICSAICSAWSQWQSTAVM
                     TGVMVNAVTATLGQVVGPPWLPLIMASAPKGTPAEARFSNAIATAISNGWMAY"
     gene            3966..4625
                     /locus_tag="PKL17_16320"
     CDS             3966..4625
                     /locus_tag="PKL17_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46350.1"
                     /translation="MPFAPQSNEVVLYPAPFVPTEHHELVISNQRVVQFAPTAMGAAL
                     PVAEFPLEKVQFVGRMTERPSAILGIVSIIVGFVFLIVFVAKVLPAFMYAGVPDKTEV
                     NLEEDSGIEGRDGNDDDPFAKDKVADEGVREKAEKKMAKVRAIKFGIPPLNEDIVWGT
                     VALIGGLIGIFLGRSMYRKQEFTVFCRVSDIVYRIPVQSEIQQSMVLSTLQAAQQSIK
                     K"
     gene            4720..5976
                     /gene="bioF"
                     /locus_tag="PKL17_16325"
     CDS             4720..5976
                     /gene="bioF"
                     /locus_tag="PKL17_16325"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNK46351.1"
                     /translation="MNPSHEGASPDDKAALPPAWQRFLAKKTQAITQAGLWRTIDAID
                     DAPGPAVKMAGLELLLFCSNNYLGLASDPRLATAASAAAQRYGVGSGASRLLSGSGPL
                     HGELERRLARLKGCEDCVLFGSGYLANVGTLAALLEPGDTVLSDRLNHASIIDGCRLS
                     GAKIEIYPHRDTDAVERILRTAQGRVLIVTDSIFSMDGDLAPLPQLCALAKAHGALLM
                     VDEAHATGVLGAGAIAHCGLSGEVPVVMGTCSKALGSLGGFIAGSRLLCNYLRQRART
                     FFFDTALPPPVLAATLTAVDIIEQEPQHGLRVLALAQRVYQRLAELGFSVLPPSGAIV
                     PVLFGPSAVAMDVAAALRSRGVLAMAVRPPTVPEGTARIRLCLMASHTDEHIERLLAS
                     FAALPPSLRRAADCQGMRQVAPNACV"
     gene            5986..8457
                     /locus_tag="PKL17_16330"
     CDS             5986..8457
                     /locus_tag="PKL17_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNK46352.1"
                     /translation="MAQRTFYLGIDLGTTNSAAAVFDGERISVVRTTQGTLLTPSIVR
                     YDSRGALTVGAKAAKFLDSDPKNTRAEFKRLMGTSTKLDFPAAKLAKLPEELSAEVLR
                     SLRNDVKDQYGFLPDQAVISVPALFELPQSAATTKAAQLAGFTKVELIQEPIASALAA
                     GWTASEGSGYWLVYDLGGGTFDASLLETRDGMLRVVGHDGDNFLGGRDFDWAIVDWLL
                     EQVSRQEGIKVSRADAKWASAIRKLKHAAEEAKIELTRSDSADISVPEFIEHDGRTIE
                     VMATLDRSQLEQLTAPLVLRSIEVCQRLLKAQQVDALTRIVLVGGPTMMPWLRQMVKD
                     ALHAPFAEGLDPMTLVAQGAAVYAATAGLDMQPAKAESSSGCKLWLQFPAMSSDMQPY
                     IVGKLLPEDKPQSALPQRIRLVRADGLWQSPDTVIDPEGGFVLQVELLPRRPNIFKVE
                     GLLADGKVMTMQPATVTIVQGLTIADPPLSRTIGVARANNTVQVFFEKGTPLPARRTI
                     SLTTVESVAPAGAGSKEEFALKIPVVQGEYEQAHLCRLVGTLEIRSSQIKASLPGGSQ
                     VQVSLEVDRGGQLSARALVPTLNQLFEHVAQLLVPEASIEVLEASLKSANDRIAELRT
                     SAFRQGQKSALERLHKPQQLVTDLERDISAAKGGDADAAQKARRSLIELDAMLEELEV
                     EKHWPELSRKAMRQVTSASFLVTRFGTDSEKRLLSDVVSSVEKAQARKDIAELQRQLR
                     LANELAMAAYYRDPDVWEDEFKEALSHVERASDQAKAQSLVQQGHQALAAGDNNKLRD
                     IVEKLWKLLPASAEQRRQGYDSGVR"
     gene            complement(8467..9108)
                     /locus_tag="PKL17_16335"
     CDS             complement(8467..9108)
                     /locus_tag="PKL17_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK46353.1"
                     /translation="MIRVVLCDDHAIVRAGIRALLERESDISIVAEAASGHEAVTLVQ
                     KLQPDLLLLDLSMPSGNGLDAIARIRQVAPRCRVLVLSMHSAPEYVRPALRAGAQGYL
                     VKGSGLSDLLRAVRVVLDGGQFLGAELAAIVSPAASLPDAPEDDLERLTPREREVLQL
                     VAEGQTNRQIASRLGLSPKTVDSHRTNLMRKLGLHDAQGVTRFAVRRGLISPE"
     gene            9167..10030
                     /locus_tag="PKL17_16340"
     CDS             9167..10030
                     /locus_tag="PKL17_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNK46354.1"
                     /translation="MRDLALAVLLCFVGCAGDAPPPPPPRPLSLLLAPDQDALHMGGT
                     GAMTPLVTLLSEEWTRRSGRPRVLVEESIGSGGGIRAAVDGAIDLGMVSRPLSSEEQK
                     LGLVTMKMGQDVVVLVAHPSVEVSSIATTDLPALYRGERTKQPSGHPLLPLLRDRSES
                     ANAALDRVVPGLYEAREQAYKQRRLRVLFSDRAMAEAIAGTPGSFGITSLSWLIANRL
                     PLKVLGIDGVVPSVENANLHRYRATRELFFVARPERLSRAQPFLDFVRTPEAQALMSS
                     YGYIPASEQRP"
     gene            10027..>10944
                     /locus_tag="PKL17_16345"
     CDS             10027..>10944
                     /locus_tag="PKL17_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNK46355.1"
                     /translation="MIPFDLTGWFRRRMLPLVALAAVVMQVSAPTAFYLTRRHELWQQ
                     AHAEAARTAMLLHGEIEQRPILWRYNVPKLLERLAGEGMLHGRFLDIRTSQGHIVPLD
                     SAPPPARPIWGRAEVSVGGELSARVWVALDMAPLLRETMQLGIAFFGLSLLLAGVLYL
                     VPVHTVSIAQKRIGTLLGELALTMQEEDRRRIARDLHDGAGQALTAARLRLLALSGQS
                     GDAANITEITKLLDNALSEVRRSVYSLSPPAITELGLHGALQRHCESFASATGLKVFC
                     ELTELPPLRPKVETACYRIVQEALANSAKH"
BASE COUNT         2125 a         3379 c         3270 g         2070 t
ORIGIN      
        1 tcggtggtgg gacgagaccc cgaggcaatg atggaaaccc ggctgctgat tgaagccaag
       61 cggccatcgg ctgatttggt ccgcgtaagc cccggtgtct tcgggctgcg cgtgtacccg
      121 ccgcgcggag aacgcggcga agcgctcaaa gcggagggag agaaagtgac caagccgaag
      181 gcggaatccg cggccacgcc cgcaaaggac ccccgagcag gcaaacgtcg tcgcaccggt
      241 cggggagcgg agagtgccga ggccccagcg gaggcagcca aaaaaccagc ggaaaccgcc
      301 gaagctgtcg cgacccctgc ggcagtggaa gccgtctccg tgccagtgac gtcggcagcc
      361 gtcgacccac ccgcaagcga agccaagccg gcgcccgcca aaggccgtgg ccggactcgt
      421 tcccgcgcta cagagacacg caaagcgccc cccgcgacgg aagcaccatc ggccaagagc
      481 agcccggcgg tagccccaga catcgcggtg gccccggtgg caccagttgt tccgacggtt
      541 ccgtcgcaaa acacaccgat ccccgcggtg gctgctgtcc cgtctcgcca gacgccggtg
      601 cccgctgccg caccagcagt tcccgtaccg aacctaaccc cagccgttcc gatcattccg
      661 acgacggaca tcgccaaagc ggcagaacca agcgcaaaaa cgccgagccc cgcgccgacg
      721 gcagcgcccg ccccggtggt cgctgtcgtg accgaaccac ccgcgagcga tctggcaaag
      781 cgaccgttcc aatccaccaa aagtccggtt ccgcttgcgc ccagcgtgcc cgccaccgcg
      841 gaatcggtcg ctgcaacggc gccagttgtc cgcccagccc cgtcggcagt cacgcctccg
      901 cgaccagtcg aagccgcggc tccgccacga cctgtcgaag ccgcgactcc gccacgcaac
      961 gaagccagcg gacatcgccc ggcgccccct ccacatgctg gcgacagacc cgcggcccca
     1021 cgcgacgaac gtcctgcgcc cagtgcgaac acgccagccc ccgcccagcc aggcccatcc
     1081 cagccgcagc agccgcgcgt gatgtcgatg gccgacgctg catacgacgt gctacgtggc
     1141 gcatccgatg gccgcgcgct gaccaatcgt cagattgcag agattgccgt caagcgccgc
     1201 ttggtccgag gcgagctggc tgacgtgtcg cgatcggtcc gcgtcgcgct tgtccgagag
     1261 cagcggcatc gagacagcga agggcttcgt ccgcggattc gcacggtcgg tccgggccag
     1321 tacacgctcg gcgagcgcaa gctcgaaccg gagctttaca atgcagaacg cgagctgctc
     1381 gatcgcgccg gtcgcacccg cgaagccacc cgcgttgccc tgcgtcgccg catccgccag
     1441 ctaccatcgg gagcgttcga gcttctgatg cggctgctcc tcgaacggat gggcatggtt
     1501 ggcgcggaga tcgtcaagcg cggcgacggg gttgcctatt acggcggcac gctgtcgcgg
     1561 ggtgcgcggt cgatcaaagt tctcgtcgca gttcgacccg gtgaaggcga gctaagcaaa
     1621 gaagctgtcg gagagctgcg tgccggtgtt cggctgcgtg gtttcgatga aggcatgatt
     1681 ctctgttctg cgcgggccgg agccgccgcc cacgccgaag ccagtgcaac gccgggggtc
     1741 gaactctatg accaagatgc gctgaccgag ctgttggttc gacaacacct tggcgtgcga
     1801 cggatgcatc tgccagtcga ctaccttgac acggagctgt tcaacgagct gctcgaacag
     1861 ggatagcaac aaatcgggaa aaccgtgggg cgacattggg ctgaactcgc acagaagact
     1921 tcagccgccc acaatcacgt tcccagatcc ttcgattagc ttcccgacac caccacagtg
     1981 ccgcgtcata tcgccaagtc gatgtgcggc cttcccattt ataaacacag tacccgatcc
     2041 cattgcggcg ttccaagtgt ttggaccaca gcaggctgcg tgtacgcctg ggtcttgaac
     2101 acgcagcgcc ggcatggaat tgacgttgac attcggcgat ccggcgattg ctgggccaac
     2161 acagctatgc acgcaagcaa cgcacccatg gccatccgcc ggaacttgtg ctttgtcgcc
     2221 gaggcgtcct tgagggggca tgtcaatctc cgttagttga tattgactgt cgagcccttg
     2281 atcgtattcg tgccactcga ctgaagcgtc atcgtaccgt tggcctttac ttcaacattg
     2341 ctgtcggcct tgatcgagta attgcttcca gcgtggcatt tcgtattttg cgagctttcg
     2401 gtttcgatat tcttggcctc gattcgaact ttctctttgg ccttgatcag aatatcgccc
     2461 gtcttgctct caatcgtgat ctttttgttc ttggtatcga taagaacgta gttctcttct
     2521 ttaccatcgt agatctcgat cttggttgct tcatttcctg cgtcattgag cacgatgcgg
     2581 tgctttgtgc tcgactgaag ggtcacgcgc gggtttgaag cgttgtcatt aaagatcaat
     2641 tcatgcccgc agcgtgagct aaaggcacga atatcattct ttttggcatc ggcttgcccg
     2701 cggaaatcgg cgtgatcttt gttatagcgg ccagtcggtg acttcgcgtc tttgttggca
     2761 aatgacggct tgtccatgcc attccacagc gaacccatca cgacgccttg gttgaagtca
     2821 ccattgatga acgcaaccat gacttcgtcg tctttctcgg gaagaaagaa cgcaccacgt
     2881 ccgcccgagc cggcaccgaa tgttccgatt cggcaccaga agctctcctc ttgctgcccg
     2941 gagccttggc tcgcgggtag cgtcgggaac ttgactttga cgcggtagtc accgccatcg
     3001 tggacgttgt ccgtgacaat tccaaccgtc atcgtgttgg cattaccacc gctgacttga
     3061 cctgctttcg tgtcacgatc ggtcttaaac atcgcgtctc ctacgatctc gaaaaaactt
     3121 tatcgcgatt tgaccatttg tgccaaatca cttaaacaaa accacctgga ggcatgttgc
     3181 caacaccgcc aacgaccggt ccaacaggaa caaacggcgg ggcaaatgtt ggtattggtc
     3241 cggttcccag tacgtttgtt acttgcgtag acgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnntgataag ccatccatcc gttgctaatt gctgtcgcaa tggcattgga
     3421 aaagcgcgct tcagccggtg tacctttggg tgcagatgcc atgatcagcg gcagccacgg
     3481 tggacccacc acctgtccaa gtgtggcagt taccgcattg accattacac ccgtcatcac
     3541 tgcggtcgat tgccactggc tccaggcgga acagattgca ctacagattc catcaatgta
     3601 ggttgcaaac tgatcaccga tcttttttgc ggtatctgta tgatatttgt tcactgtggc
     3661 aggaacaaac atcggcggag ccggtggaat tcccaccagt tccgacgatt tgaacgctcg
     3721 cgtgtactgc ttaccagcct cgccttgcgg ctgtttccag tcggtaggca gcttggtagc
     3781 gaagctaatg aacttcgcct ttgcgaggtt ttgcattacc gaggcttgcg gcgctggcat
     3841 acagtcttcc ccactttcac tttactaaga cacgctaccg atcggcaacg gcatagccgc
     3901 cccagtgacc caatttgcaa acccccgaca cagcactacg catcatgagc atgttacgat
     3961 gacgcatgcc cttcgccccg caatctaacg aggtcgtcct atatcccgcc ccgtttgttc
     4021 cgaccgagca ccacgaactt gtgatcagca accagcgcgt cgtgcagttt gctccgacgg
     4081 caatgggcgc cgcgctcccc gtggcggagt ttccgctcga gaaggtccag ttcgtgggtc
     4141 gaatgacgga gaggccgtct gcgatcttgg gcattgtctc gataatcgtc gggtttgtct
     4201 ttcttatcgt ctttgtggcg aaagtgttgc cggccttcat gtatgccggt gtgcccgaca
     4261 agaccgaagt gaacctcgag gaagactcgg gcatcgaagg ccgcgacggc aacgacgacg
     4321 accccttcgc caaagacaag gtcgccgatg aaggcgtgcg cgaaaaagcc gaaaaaaaaa
     4381 tggccaaagt cagggccatc aagttcggga ttccgccgct caacgaagac atcgtctggg
     4441 gcaccgtcgc gctgatcggt gggttgatcg gtatttttct tgggcgaagt atgtaccgaa
     4501 agcaggaatt cacggtcttt tgccgagtca gcgacatcgt ctatcgcatt ccagtccaaa
     4561 gtgaaatcca gcagagcatg gtgctgtcga cgctgcaggc tgcacagcaa tcgatcaaaa
     4621 agtagccgcc cgcagccgat ctgccccgcc ccgccccgct gtcaccagcc ttcactttcc
     4681 cagctttcgc ggaacacgcc tgcggcaaga tgcgcagcaa tgaacccatc gcacgaggga
     4741 gccagtcccg acgacaaagc ggcactgcca ccggcttggc agcggttttt ggcaaaaaag
     4801 acgcaagcga tcacccaagc cggactgtgg cggacaattg acgccatcga cgatgcgcca
     4861 gggcccgcgg tgaaaatggc cggtctggag ctgctgctgt tctgttccaa caattacctg
     4921 ggacttgcga gcgacccgcg acttgcgacg gcggcaagtg cggcggcgca gcgctatggc
     4981 gtcggtagtg gtgcctcgcg gctgctcagc ggcagtggac cgctacacgg cgagctcgaa
     5041 cgacggctcg cgcggctgaa aggctgtgag gactgcgtgc tgtttggctc gggatatctc
     5101 gcgaatgttg gaacgctggc ggcgctgctc gaaccgggtg ataccgtgct gtccgatcgg
     5161 ctcaatcacg catcgatcat cgacggatgc cgcctgtccg gggccaagat cgaaatttat
     5221 ccacaccgcg acaccgatgc cgtcgagcgc attctgcgca cagcccaagg ccgggtgctg
     5281 atcgtcacag acagcatctt ttcgatggat ggcgatctgg cgcccctgcc acagctgtgc
     5341 gcgctggcca aagcccacgg ggcgctgctg atggtcgacg aagcccacgc aacgggcgtg
     5401 ctcggagcag gagcgatcgc gcattgtggt ctttctggtg aagttcccgt cgtgatggga
     5461 acgtgctcca aggcgctggg ctctctcggt gggtttatcg ccgggagccg actgctgtgc
     5521 aactatctgc gccagcgtgc ccggacgttt tttttcgaca cggcactgcc gccgccggtc
     5581 ttggctgcga cgctgaccgc agtcgatatc atcgaacagg agccgcagca cggcctgcgc
     5641 gtcttggccc tggcgcagcg ggtgtatcaa agactcgccg agcttggttt ttctgtcctg
     5701 ccgccaagcg gtgcgatcgt gccggtgctg tttggaccgt ccgccgtagc catggatgtg
     5761 gccgcagcgc tgcgcagtcg tggtgtgctg gcaatggcgg tgagaccccc gacggtgccc
     5821 gaaggaactg cacgcattcg gctgtgcctt atggcttcgc ataccgacga gcacatcgaa
     5881 cggctgctgg caagcttcgc cgcgcttccg ccatcccttc gtcgcgcagc ggactgccaa
     5941 gggatgcggc aggttgcgcc aaacgcttgt gtgtaagata tccgcatggc gcagcgaacc
     6001 ttttacttgg gcatcgatct ggggacgaca aactcggcag ccgcggtctt cgatggcgag
     6061 cggatttcgg tcgttcgcac cacgcaaggc acactgctga ccccatcgat tgtccgctac
     6121 gacagccggg gcgcactcac cgtcggggcg aaagctgcaa aatttctcga tagcgatccg
     6181 aagaataccc gcgccgagtt taagcggctg atgggaacgt cgaccaagct cgattttccc
     6241 gccgcgaagc tggccaagct ccccgaggag ctgtccgctg aagtgctgcg atcgctgcgc
     6301 aacgacgtca aagatcagta cgggtttctg cccgatcagg ccgtgatttc agttcccgcc
     6361 ttgttcgagc ttccgcaaag tgctgcgacg acaaaagccg cgcagcttgc aggttttacc
     6421 aaagtcgagc tgattcaaga accgatcgca tcggcgctgg cagctggctg gaccgcatcg
     6481 gaaggctcgg ggtattggct ggtctacgac ctgggtggcg gcacgtttga tgcgtcgctt
     6541 ctcgagacgc gcgatggcat gctgcgtgtg gtcggccacg acggcgacaa cttcctgggg
     6601 ggacgggatt tcgactgggc aattgtcgat tggctgctgg aacaagtgtc gcgccaagag
     6661 ggcatcaaag tcagccgcgc cgacgccaag tgggccagtg cgatccgcaa gctgaagcac
     6721 gccgccgaag aagccaagat cgagctaacc cgcagcgaca gcgccgacat cagcgtgcca
     6781 gagtttatcg agcacgacgg tcgcaccatc gaagtcatgg cgacgctcga tcgcagccag
     6841 ttggaacagc tcacagcgcc gctggtgctg cgatccatcg aagtctgtca gcgcttgctc
     6901 aaagcccagc aagtcgacgc gctgacgcgg attgttcttg tcggtggtcc gacgatgatg
     6961 ccgtggctgc gccagatggt caaagacgcg ctccatgctc cgtttgccga agggctcgat
     7021 ccgatgacgc tggtcgcgca aggtgccgca gtctatgcag cgacggcggg cctagacatg
     7081 caacccgcca aggcggagtc gtcgtctggc tgcaagctgt ggcttcagtt tcccgcgatg
     7141 tcatccgata tgcagcccta catcgtcgga aagctgctgc cggaagacaa accccaaagc
     7201 gcgctgccgc agcggattcg cttggtccgc gcagacggtc tgtggcaaag ccccgatacg
     7261 gtgatcgatc cggaaggtgg atttgtgctg caagtcgagc ttttgccccg tcgcccaaac
     7321 atcttcaaag tcgaaggtct tttggccgat ggaaaagtta tgacgatgca gccagcgacg
     7381 gtaacgatcg ttcaaggact gacgattgcc gatcctccgc tgtcccgcac gattggcgtg
     7441 gcgcgggcga acaacaccgt gcaggtgttt tttgaaaaag gaacgccgct gccggcccgc
     7501 cgcacgatct cgctaaccac cgtcgaaagt gtcgcgccag cgggagcggg atcgaaagaa
     7561 gagtttgcgc tgaagatccc cgtcgtccaa ggtgagtacg agcaggctca tctttgtcgt
     7621 ctcgtcggaa cgctggaaat ccgcagctcg cagatcaaag ccagcttgcc gggcggatcg
     7681 caggtgcagg tcagcctgga agtcgatcgc ggcggacagc tatcggcgcg ggcgctggtt
     7741 ccgacgctaa accagctgtt tgagcatgtc gcccagctgc tggtgccgga agccagcatc
     7801 gaagtgctcg aggccagcct gaagtcagcc aacgatcgca tcgccgagct gcgcaccagt
     7861 gcatttcgcc aagggcaaaa gtcagcgctc gagcggctgc acaaaccaca gcagcttgtg
     7921 accgatttgg aacgcgacat cagcgccgcc aaaggtggtg acgccgacgc tgcccagaaa
     7981 gcccgtcgct cgctgattga gcttgatgcg atgctcgaag agctggaagt cgaaaagcac
     8041 tggcctgagc tgtcgcgcaa agcgatgcgg caggtgacga gcgccagttt tctggtgacg
     8101 cgctttggta ccgattcgga aaaacggctc ttgtcggatg tggtttcgtc ggtggaaaaa
     8161 gcgcaagcgc gcaaagacat tgccgaactt cagcgccagc tgcggctcgc caacgaactg
     8221 gcgatggcgg cgtactatcg cgatcccgac gtgtgggaag acgagttcaa agaagcgctg
     8281 tcccacgtcg agcgcgcgag cgatcaggcc aaagcccaga gcctggttca gcagggtcac
     8341 caagcgctcg cggcgggcga taataacaag ctgcgcgata tcgtcgaaaa gctgtggaaa
     8401 ctgttgccag cgtccgccga gcagcgtcgg caaggctacg actccggcgt gcgctagacg
     8461 gcccgcttat tcgggcgaaa tcagacctcg ccgcaccgca aagcgggtga cgccctgcgc
     8521 atcgtgcagg cccagcttgc gcatcaggtt ggtgcggtgc gagtcgacgg tctttggaga
     8581 cagtccaagc cgagaggcaa tctgccggtt ggtctgccct tccgcgacaa gctgcaagac
     8641 ctctcgctct cgtggggtca gccgttcgag gtcgtcttcg ggcgcatcgg gcaagctcgc
     8701 agccggtgac acgatcgccg caagctcagc gccgagaaac tgcccaccgt cgagcaccac
     8761 ccgcacggcg cgcagcagat cggacagtcc gctgcctttg accagatagc cctgcgcccc
     8821 ggctcgcagc gccggacgga catactctgg cgccgagtgc atcgacaaca ccagcacccg
     8881 acagcgcggc gcgacctgtc gaatccgcgc aatcgcatcc aggccattgc cgctcggcat
     8941 cgacaaatcg agcagcaaaa ggtctggctg gagcttttgc acaagcgtca ccgcttcatg
     9001 tccagacgcg gcctcggcga caatcgaaat gtcgctttcc cgttccagca gagcacgaat
     9061 ccccgcccga acgatcgcat ggtcatcgca cagcacgacc cgaatcacag tttcacgata
     9121 acacttcgcg cggccagact tttgccggtc cgcctatgat gaagctgtgc gcgacttggc
     9181 gttggccgtt ctgctttgtt ttgtgggctg tgcgggggat gcgccaccgc cgccgccgcc
     9241 gcgaccgctt tcgcttctgc tcgcgcccga ccaagatgcg ctgcacatgg gcggaaccgg
     9301 ggcgatgacc ccgcttgtga ctctgctttc tgaagaatgg acgcgccgat cgggcagacc
     9361 gcgcgtcttg gtcgaagaaa gcattggatc gggcggtggc atccgcgccg cagtcgatgg
     9421 cgcgatagac cttggcatgg tgtcccgtcc gctcagcagc gaagaacaaa agctgggtct
     9481 tgtgacgatg aaaatgggac aagacgtggt ggttttggtc gcccatccca gcgtcgaagt
     9541 cagcagcatt gcgacgacgg atctgcccgc gctgtatcgc ggtgagcgca ccaaacagcc
     9601 cagtggccac ccactgttgc cgctgctccg cgatcgcagt gagtcagcca atgcagcact
     9661 cgaccgcgtc gtgcccggtc tttatgaagc gcgcgaacaa gcctacaagc agcgtcgcct
     9721 gcgggtgttg ttcagcgatc gcgccatggc cgaagccatt gcgggtacgc ccggttcctt
     9781 cggcatcacg tcgctgagct ggcttatcgc caatcgcttg ccgctcaaag tgctgggcat
     9841 agatggcgtg gttccgtcgg tcgaaaacgc caatttgcat cgctatcgcg ccacccgcga
     9901 gctgtttttc gtcgcccgcc ccgagcgact ttcgcgcgcc cagccgttcc tcgactttgt
     9961 acgcaccccg gaagcgcaag ccctgatgtc gagctatggc tatatccccg cgtcggagca
    10021 gaggccatga ttccgtttga tctgacgggc tggtttcggc gacggatgct cccgctggtg
    10081 gcgctcgcgg cagtggtcat gcaagtgtca gctccgacgg cgttttatct gacccggcga
    10141 cacgagctgt ggcagcaggc ccacgccgaa gcggcccgca cggcgatgct gctgcatggc
    10201 gaaatcgagc agcgaccgat cttgtggcgc tacaacgtgc caaagctgct ggagcggctc
    10261 gcgggcgaag gcatgctgca tggtcgattt ctcgacatcc gcaccagcca aggacacatc
    10321 gtgccgctcg attcggcccc gccgccagcc cgtccgatct ggggtcgcgc cgaagtcagc
    10381 gtcggtggcg agctgtcggc ccgcgtctgg gtggcgctcg atatggcacc gcttttgcgg
    10441 gagaccatgc agcttgggat cgcgtttttc ggcctgtcgc tgctgctcgc gggggtgctt
    10501 tatcttgtgc cggttcatac ggtgtcgatc gcgcaaaaac gcatcggcac cctgcttggc
    10561 gagctggcgc tgacgatgca agaagaagat cgccggcgca ttgcccgtga tctccacgac
    10621 ggagccggcc aagccctgac cgccgcgcgg ctgcgactgc tcgcgctgtc gggacagtcg
    10681 ggcgatgccg ccaacatcac cgagatcacc aagctgctcg acaatgcgct aagcgaagtc
    10741 cgacggtctg tctattcgct gtcgccaccg gcaatcaccg agctgggact tcacggcgcg
    10801 ctgcaaaggc actgcgaaag cttcgcatct gcgacgggac tgaaagtctt ctgcgaactg
    10861 accgagctgc cgccgctgcg tcccaaagtc gaaaccgcct gctaccgcat cgtgcaagaa
    10921 gcgctcgcca acagtgcaaa acac
//
LOCUS       DAORGN010000171        10943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2530_length_10943_cov_5.374082, whole genome shotgun sequence.
ACCESSION   DAORGN010000171 DAORGN010000000
VERSION     DAORGN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_10943_cov_5.374082"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKL17_16350"
     CDS             complement(<1..367)
                     /locus_tag="PKL17_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46356.1"
                     /translation="MNRLLILFGLLWLACSPTVEMLSDPDPGLADLAITDVFPPTILP
                     ASLLVIRGRSLPGPGDGSIHLWLSGTSKLPGTVPRSLDVRLRAEYVSAQEAQLRIDTE
                     VLQALGGNSVEFSGHAKIVV"
     gene            complement(364..1026)
                     /locus_tag="PKL17_16355"
     CDS             complement(364..1026)
                     /locus_tag="PKL17_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46357.1"
                     /translation="MRALLFAALLFASACGPSGGSLALSAIAPHSASVAQEVVIPLLA
                     DGAGTFPIGWAWQSPSNPELATRLHRPTLSAYSGGRALWRFTPLATDVGPLQIEFSAT
                     KGPLSGSLTLDFVIDPGGDPPVFREPVGEGTTLDLKAMPCAVVSVVVESDSSPQVTLS
                     LLNPPPNAEVAQTEELAGKLVFCPSATQAMTETIYPLLLQAEAGSHIVRKSYVIVLRR
                     SA"
     gene            complement(1210..1437)
                     /locus_tag="PKL17_16360"
     CDS             complement(1210..1437)
                     /locus_tag="PKL17_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46358.1"
                     /translation="MKKLASLFAFAFALSIVAPAMAEETGAPAEKAAEKKDEKKAEKK
                     AEEKKGAHKKAKEEKKEEKKEEKKEEKKAEK"
     gene            1801..2112
                     /locus_tag="PKL17_16365"
     CDS             1801..2112
                     /locus_tag="PKL17_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNK46359.1"
                     /translation="METSQHPKRIHLLISGKVQGVYYRSSACEKARVLGLCGTVRNLP
                     SGQVELVAEGEESALQRLIAWCQMGPPAAEVSAVDVRYETTTGDFRDFQVVRHSPQLN
                     R"
     gene            complement(2117..2614)
                     /locus_tag="PKL17_16370"
     CDS             complement(2117..2614)
                     /locus_tag="PKL17_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46360.1"
                     /translation="MRTNNKTALTVAMGMMLVAPAVFGQAAPAPASAPAPAPAPAADA
                     KASKGEVKAGNAQIAADKAAIKADKAEIKKDRQEIKADKAEIKADKQAIKDARAAGDK
                     EGLKAAKAELKADAKELAKDKKDMAHDKKELARDKKELAHDKKEAKHDAKDARHDMKD
                     AKPAK"
     gene            2929..3570
                     /locus_tag="PKL17_16375"
     CDS             2929..3570
                     /locus_tag="PKL17_16375"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK46361.1"
                     /translation="MPGEPRGGPEVAQLIRNAQHGDIAAFEQLMAMHKSKILSYARAF
                     VSDAEQAHDVAQDAMLRIYRSLGSFRHQSSLQTWMFRIIRNIVLDYAKSRRSKERKRE
                     QPLDKTPESDMGEPASDSPEAQLLAHERKSQLWLALAEVPETYRSVLVLCDLQGMSYE
                     EVAAIVSTPVGTVKSRLNRGREALRLTLLAQGHYGDDVPAPQSEHPRAEGKKP"
     gene            3567..4274
                     /locus_tag="PKL17_16380"
     CDS             3567..4274
                     /locus_tag="PKL17_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNK46362.1"
                     /translation="MTCADCTKQLALLVSGDLSPVSRAAIDQHLSHCPDCRRHLNQLE
                     KTVLLLKKAGQDMTVPSDFSTRLHARLLQEPAPRIPLSTKLWWKLESLGLDSGPRLGA
                     CAMAFAVLVLLFIGTLRGGGPGRATVFVPEETVAASFRIPSHRVAVVQLDFVADASVD
                     DVEFEITLPGELEFIDGGQAISERHLSWRGSLAIGSNPVPVAVRGARPGLYRVTAQAK
                     GRDVTVRHEVMLEVVPS"
     gene            4271..4813
                     /locus_tag="PKL17_16385"
     CDS             4271..4813
                     /locus_tag="PKL17_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46363.1"
                     /translation="MKSLLLCFPVSSMLWVAAASAQTAQPKDKEPSRPPQVRTYSTVT
                     VVDDPTKAPRLPTQKQAPQPAKETPTAAKDAQTPVPSAKEPASQPKELVPASRSDSKS
                     AMKDVASADRKNIDLLRQDLRSTAKELRESQKRDTIRDPGIGATPAPKRTAPDQTPST
                     ESRREHRLRQILQERAGRDN"
     gene            complement(4842..4914)
                     /locus_tag="PKL17_16390"
     tRNA            complement(4842..4914)
                     /locus_tag="PKL17_16390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4879..4881),aa:Ala,seq:tgc)
     gene            complement(5002..7182)
                     /gene="lnt"
                     /locus_tag="PKL17_16395"
     CDS             complement(5002..7182)
                     /gene="lnt"
                     /locus_tag="PKL17_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNK46364.1"
                     /translation="MSGPQTAENATPSDPPLPSDPSPSDPSPSSSPDVVSRRSGALPR
                     FLIPLVESVWGRLLLCVLSGTLLFLSCADFDIWPLCWIGMVPLLWVLLDDDGLPRTKN
                     PFFYGMVTGIFANGGGFYWIVGLLQRFGHLPWIAAAPLFLLLVAYQALTFGLFALIVC
                     RLRAHIGVKVTWLAPLVWVACELCVPYVFPWYLSITQAWVPTVIQIADITGPLGVSFL
                     LVLSNGALFDVLHALGRAKPGRRLRAFLGAPLLAVATIVLCLLYGTVRIRQVDAQRAS
                     AEKVRVGVVQANVGIHEKFVPGLREQQHNLHLSLSKELSDKGADLLVWPESSYPYPLL
                     RPVPGDFSDRDARKVQRGYSTPIFFGALTVGRTPDGDMDIYNTAVLFDKQGKTVGTFD
                     KNFLLIFGEYLPFAHELAWLRELIPAISNFSRGTETTTFPFELRGKRYSLGPMICYED
                     IIPAFGRRLFSDKNPPNLMVNITNDAWFGATSEPYEHLALAVYRAVEHRVELVRAVNT
                     GVSAFIDAAGRVYQKGPSVDPQLQPDAKPVTLLGDMALLPPGGLYQRLGEAFGFGCLT
                     IVILLGALSRQRAGSPLRLGLIVFGSATLHLAAVGAGLLLPAGTSALYAVLLHRGENA
                     LPESVAFAANWQLLLALALASAGLGGYAGRQRMARAPQLELIGAICATTVLPVTLFGR
                     MEGNTGAVVIVSGLCVLSSLIAVKWSSARRNRATKPSKVSGSAN"
     gene            complement(7243..8946)
                     /locus_tag="PKL17_16400"
     CDS             complement(7243..8946)
                     /locus_tag="PKL17_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNK46365.1"
                     /translation="MKANAKPAYTDLMKRPLCASVLGLLIAQSPSVHAEPTCRGVEVR
                     FTPQGMPPKNGREELRPQIAVWIEKNDGSFLTDLFVTRSIGMLGLGNRPGNQWLKSDF
                     RWPYGRRPQSLPVWAYRRGKTYPFISMGGKCSPTCPPDFQGSPSDDDNTVAYHGPVSS
                     TENYYCSPSGWRTQKMNGVDVVSCASAFYGSKGYYAAGKTSVYPPRADLSTVGGNDSP
                     DVKRFAMDNDVAAISGATPANGKLIDPIRWFADAQIPDDDYVLYVEVGIEGDFNAYWP
                     TGRAMAEPHAEWDHLGKDPYGQPSIVYKVPFRLDKSGRISTTKDYAGYGDFKGENGKL
                     NPPDSTISQSGGSGADRLAMNTDDNGTWRVKVSVSPCDPGMCDMPSPPTKLALSEVTD
                     TTFRLGFAVPAGAPASEYRVRYQSTGPITAENFARALPAPSANLGPPGAMVGSQIVGL
                     LPKTKYYIAVQPVSRCGAPGTIVTTEVDTSSANFTTLTGCFIATAAFGSELEPEVNVL
                     RQVRDKYLVKSPLGLGLIGSYYTISPGVARLVAEHPPLRNLVRETLKPVVRVLHLTTG
                     L"
     gene            complement(8943..10631)
                     /locus_tag="PKL17_16405"
     CDS             complement(8943..10631)
                     /locus_tag="PKL17_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK46366.1"
                     /translation="MERAHDPTAWWQKTTVYQIYPRSFFDANGDGIGDLPGILAKLDH
                     MSSLGVETLWLSPFFESPQRDFGYDISSYARVSADHGTWADFETLISEVHRRGMRIVI
                     DMVLNHTSDQHPWFRAALRSKDSPERDYYLFRPGKGPGKNQPPTNWRSMIGPRGWHRD
                     GDSGDYYFASFLPFQPDLNHRNPTVQRAMFDIIERFVRLGVDGLRLDIFNALYKDDRF
                     LDNPPSLRPLPSEDNADGFFQRNLYTINHPDSIAFARTLRAFVDSLPGGPRFLVGEVF
                     GPPSLLRSYCGQHADGLNLVFLFKTLRTEFSAAAFYALIEEYERDFADPLLPTWVLGN
                     HDRARYIERVGDDPRRAKVLATLQLSARGVPFVYQGEEIGMTNLDLPIATALDPLAHF
                     YRFVPPWLQRLLRRKGVLINRDECRTPMQWTADDNAGFSPPGKRPWLPVHPSYPWTNV
                     AAQLHDPQSLLAHYRRLFQLRQKTPALHAGRLVLWHPTNLPRDVLGYRREHGDDRLDV
                     LLNFSDARREVSIPQPHHVLFTSHPDAPQTAGSTLRLRPFESVILRREAQGVGR"
     gene            complement(10657..>10943)
                     /locus_tag="PKL17_16410"
     CDS             complement(10657..>10943)
                     /locus_tag="PKL17_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA pseudouridylate synthase"
                     /protein_id="HNK46367.1"
                     /translation="DRPPARKYEDKPAPRRFDDRPPARKYEDKPAPRRFDDRPPARKY
                     EDKPAPRRFEDKPAPRRTDERPAPRRFDEKPTGRAKFPRSRPNQPPPPLD"
BASE COUNT         2309 a         3448 c         3153 g         2033 t
ORIGIN      
        1 cgacgacaat tttggcgtga ccggaaaact caacactgtt tccgcccagc gcttgcagca
       61 cttcggtatc aattcgcaac tgggcttcct gcgcgctcac atactcggca cgcaggcgga
      121 catcgagact gcgcggcacc gtccctggca gcttgctcgt gccagaaagc cacaggtgaa
      181 tcgatccgtc accgggtccg ggcagtgacc gtccgcgaat caccaagagc gacgcgggca
      241 aaatcgtcgg tggaaacacg tcggtaatcg ccaagtccgc aagccctgga tcgggatcac
      301 tgagcatttc gaccgtcggt gaacaagcca gccagagcag cccaaataaa atcagcaggc
      361 gattcatgcc gacctccgga gcacgatcac gtagcttttg cgaacgatgt gtgaccccgc
      421 ttccgcttgc agaagcagcg gatagatcgt ttctgtcatc gcttgggtgg ccgatgggca
      481 gaacaccagc ttgccggcga gttcttccgt ctgggcgact tctgcattcg gcggtggatt
      541 gagaagcgaa agcgtcactt gcggagagct atcggattcc acgaccaccg acaccaccgc
      601 gcacggcatc gctttcaaat cgagcgtggt tccctcgccg acgggttcgc gaaagacggg
      661 cggatcgccg ccgggatcaa tcacaaagtc caaggtcaaa gaccccgata gcggaccctt
      721 tgtcgccgaa aactcgatct ggagcggccc gacatcggta gcaagcggcg taaagcgcca
      781 cagcgcccga cccccgctgt acgcgctcag cgtcggacga tgcagccgag tcgccagttc
      841 cgggttgctc ggtgactgcc aagcccagcc gatcggaaac gtccccgccc catccgcgag
      901 cagcggaatc acgacttctt gggcgaccga agccgagtgc ggcgcaatcg ccgatagcgc
      961 aagcgagccg cccgaaggac cacacgcaga tgcaaacagc agcgcggcaa acagcaaagc
     1021 tctcacgtcg agagcataca cagaaacccg atcgcgttgc gcagggattg agaagtcagc
     1081 gccccaaaac caaccaagca acgccgcaat cgaaccccca aaatgcaaag agcccactcc
     1141 atcactggag caggctcttt gttggatcga agaaacgggc ggtgcccgct atcgagccga
     1201 aggcgaagac tacttctcag ccttcttctc ttccttcttc tcttccttct tctcttcctt
     1261 cttctcttcc ttcgccttct tgtgagcgcc cttcttctct tcggccttct tctcggcctt
     1321 cttctcgtcc ttcttctcgg cggccttctc agccggggcg ccggtctcct cggccatggc
     1381 cggggcaacg atcgagaggg cgaaggcgaa cgcgaacagg ctggcaagct tcttcatggg
     1441 atgtctccta tcagattggg tttgaggtgc agcgagcgtc acgcccgcac acgctgtctt
     1501 ggcctcttgc ggaaaccatg ccaagccatc gtgggcttga ccccaaggca atcggaaacg
     1561 cccatttccg ccgagaacac cgccacatcc acattacgag tccgctctgc gcgtcgcatg
     1621 taaggtgagc cgttcagtgg ggcaaaacga ctcatgaccg aagtgccaca cgcaatcacg
     1681 cctcattgcg cgaagaatcg acaagctgcg ctacgcttgt ggtcatagag attgaaccac
     1741 caaaccaaaa cgaccacgct tgcacagaca aactcgcctg cctaaaacgg agtcgttgcc
     1801 atggaaactt cgcagcaccc aaaacggatt cacttgctga tcagcggcaa agtccagggc
     1861 gtgtactacc gctcgtctgc ctgcgaaaaa gcgcgggtgc tcgggctgtg cggcacggtc
     1921 cgcaaccttc caagtggtca agtagagctg gtcgcagaag gcgaagaatc ggcactacag
     1981 cgtctgattg cgtggtgcca gatgggaccg ccggcagccg aggtgtccgc ggtggatgtc
     2041 cgctacgaaa caactacggg ggactttcgc gacttccagg tcgtgcgtca ctcgccgcag
     2101 ctgaatcgat agccgactac ttggccggct tggcgtcttt catgtcgtgg cgcgcgtctt
     2161 tggcgtcgtg cttggcttcc ttcttgtcgt gcgcaagctc ttttttgtca cgagccagtt
     2221 ccttcttgtc gtgcgccatg tctttcttgt ccttggccag ctccttggca tcagccttca
     2281 gctcggcctt ggccgccttc agcccttctt tgtcaccggc agcgcgggcg tctttgatcg
     2341 cctgcttgtc ggccttgatc tcggccttgt cggccttgat ttcctgacgg tctttcttga
     2401 tctcggcctt gtcggctttg atcgcggctt tgtcggcggc gatctgcgcg ttgccggcct
     2461 tgacttcgcc cttgctggcc ttggcgtccg ccgccggagc tggagccgga gccggggctg
     2521 aggccggggc tggagcagcc tggccaaaca cagccggcgc aaccagcatc attcccatcg
     2581 caacagtgag agcagtcttg ttattggtcc gcatggtggt gtcctcccta tttctgtgat
     2641 ccgaaagcgg tgcttgttag cccgttcatg agcaaggaga cgaggcgact cgcccgtttg
     2701 ttccaagcaa cgcgggtttc tgaaaatgta ttagaaatta cattagatat aaattccgtt
     2761 tcgcaagatt cttattttca ggacgagacc agccggtctc tgaccgcatc ccggaaccaa
     2821 tggcagcgcc gcctcgtctt ttgttcacag cccatgcaag ccgtcctccg ctttccaggc
     2881 cgcgacctcg caagtgtcgc aacgaccgcc gccgaatcgg agccaagcgt gcccggtgag
     2941 cctcgcggcg gccccgaggt ggcccagctc atccgcaacg cccagcacgg agacatcgcc
     3001 gcgtttgaac agctgatggc gatgcacaaa agcaagattc taagctacgc gcgggccttc
     3061 gtttccgacg cagaacaagc gcatgacgtg gcgcaagacg cgatgctgcg aatctatcgt
     3121 tcgctcggga gctttcgcca tcagtcgtcg ctgcagacgt ggatgttccg aatcattcgc
     3181 aacatcgtgc tcgactacgc caaaagccgt cgcagcaaag aacgcaaacg cgagcagccg
     3241 ctcgacaaaa ccccagaaag cgacatgggc gagcccgctt ctgacagtcc ggaagcccag
     3301 cttctggcgc atgagcgaaa gtcacagctc tggctggcgc tggccgaggt tccagagacg
     3361 taccgaagcg tgctcgtgct gtgcgatctg caagggatga gctacgaaga agtcgccgcc
     3421 atcgtttcta cgcctgtcgg gacggtgaag tctcgattga atcgcggacg cgaagcgctg
     3481 cgactcaccc tgcttgcgca aggccactat ggcgacgacg tgccagcgcc gcagagcgaa
     3541 catccacgag ccgaggggaa gaagccatga cgtgcgcgga ctgcaccaag caacttgcgc
     3601 tgctcgtgag tggcgatttg tcgccagtga gtcgcgcggc gatcgatcag cacttgtcac
     3661 actgcccgga ctgccgacgg cacttgaatc agcttgaaaa gaccgtgctg ctgctgaaaa
     3721 aggcgggaca ggacatgacc gttcccagcg atttctcgac gaggctgcat gcacgtcttc
     3781 tgcaagaacc ggcgccacgc attccgcttt ccaccaagct gtggtggaaa ctggaatcgc
     3841 tggggctcga tagtggtccg cggcttggag cctgcgcgat ggccttcgcc gtgctggttc
     3901 tgttgttcat cggaacgctg cgcggcggtg gtcctggccg agcgactgtc tttgtccccg
     3961 aagaaaccgt cgctgcatcg tttcgcattc ccagccatcg cgtcgcggtc gtgcagctcg
     4021 atttcgttgc cgatgcgtcc gtcgatgatg tcgaattcga gatcacgctt cccggtgagc
     4081 tagaatttat cgacggtgga caggcaattt cggaacggca cttgtcgtgg cgtggttctc
     4141 tcgcgattgg ttcgaatcca gttcccgtcg cggttcgtgg tgcccggccc ggcctctacc
     4201 gcgtcactgc gcaggccaaa ggccgcgatg tcacggtccg tcacgaagtc atgctagagg
     4261 tggtgccgtc atgaagtcgc tcctcctctg ctttccggtc agcagcatgc tgtgggttgc
     4321 tgcagcgtcg gcacagaccg cccagcccaa agacaaagag ccgagtcgtc cgccgcaggt
     4381 ccgtacctat tcgacggtaa cggtggtcga tgatcccacc aaagcaccgc gactgccgac
     4441 gcaaaagcag gcgcctcaac cggccaaaga gaccccgacg gcagcaaaag acgcccagac
     4501 tcccgtgccg tcggccaaag aacccgccag ccagcccaaa gaactggtcc cagcaagccg
     4561 cagcgattcc aagtcggcga tgaaagatgt ggcgtccgcc gatcggaaga acatcgatct
     4621 gctccggcaa gacctgcgct cgacggcaaa agagctgcgg gagtcgcaaa aacgggacac
     4681 catccgcgac ccaggaatcg gggcgacccc ggcgccgaaa cggacagcgc ccgaccagac
     4741 accaagcaca gaatcgcgac gggaacaccg tctgcgtcag atcctgcaag agcgcgcggg
     4801 tcgcgacaac tgactgtcgc tcactttagc cgtccgcccg gtggaggaaa tgggactcga
     4861 acccacgaca cccggcttgc aaagccggtg ctctaccaac tgagctattc ccccgaagaa
     4921 acaaaacttg cagttgcaag tttgggcggg cggctgaagt ggtgaaaact atagccaaat
     4981 cggcaaacgc ctgacaaggc gttaatttgc actgccgctg actttgcttg gttttgtggc
     5041 ccggttgcgg cgcgcgctgc tccatttgac cgcgatcagc gacgacagca cgcacaatcc
     5101 agaaacaatc acgacagcgc cggtgttgcc ttccatgcga ccaaacaacg tcaccggcag
     5161 cacggtcgtc gcgcagatcg caccaatcag ctccagctga ggcgcccgcg ccatccgctg
     5221 ccgtccggca tagccaccaa gccccgccga agccagcgca agcgccagaa gcagctgcca
     5281 gtttgccgca aacgcaaccg actcaggcag cgcattttcc ccgcggtgaa gcagcacggc
     5341 gtacagcgct gaagttcccg ccggcaatag cagtcctgct ccgaccgctg ccagatgaag
     5401 cgtcgccgat ccgaagacga taagcccaag ccgtagcggc gatcccgccc gctgccgcga
     5461 caacgcaccc agcaaaatca cgatcgtcag acagccaaaa ccaaacgctt ccccgagtcg
     5521 ctgatagagc ccgcccggcg gcaaaagcgc catgtcaccg agcagcgtca ccggcttcgc
     5581 atcgggctgg agctgtgggt ccaccgacgg tcctttttga tacacccgac ccgctgcatc
     5641 gataaatgcc gatacgccgg tgttgaccgc acgcaccaat tcgacgcgat gctcgacggc
     5701 gcgatagacc gcgagcgcca ggtgttcata aggttccgac gtggcgccaa accacgcatc
     5761 gttggtgatg ttgaccatca ggttcggcgg gtttttgtcg gaaaacagcc gtcgcccaaa
     5821 cgccggaatg atgtcttcgt aacagatcat cgggcccagc gaatagcgct tgcctcgcag
     5881 ctcaaacggg aaggtcgtcg tctcggtgcc gcgcgaaaaa ttggaaatcg ccgggatcag
     5941 ctcacgaagc cacgcaagct cgtgggcaaa cggcagatat tcgccaaaga tgagcaggaa
     6001 gtttttgtcg aacgtaccga cggtcttccc ctgtttgtca aacagcaccg cggtgttgta
     6061 aatgtccatg tccccgtcgg gcgttcgccc caccgtcagc gcgccaaaga agatcggggt
     6121 cgaatagcca cgctgcactt tgcgggcgtc acggtcggaa aaatcccccg gcacaggtcg
     6181 caacagcgga tacggatacg acgactcggg ccagaccaaa agatcggccc ccttgtcgga
     6241 cagctctttg ctcagcgaca gatgaagatt gtgctgctgc tcacgcagac ccggcacgaa
     6301 cttttcgtgg atgccgacgt tggcctggac aacaccgacg cgcacttttt cggcgctcgc
     6361 acgctgcgca tcgacctgcc ggatgcggac cgtcccgtac aaaaggcaca acacgatcgt
     6421 cgccaccgcg agcagcggcg ctcccaaaaa tgcccgcagc ctgcgccccg gcttcgctcg
     6481 tccgagcgca tgaagcacgt cgaaaagcgc cccatttgac agcaccaaaa ggaagctcac
     6541 gccgagcggt ccggtgatgt ccgcaatctg aatcaccgtc ggaacccacg cctgcgtgat
     6601 cgacagatac cacgggaata cgtagggcac gcacagctca caggcgaccc agaccagcgg
     6661 cgcgagccac gtcaccttca ccccaatgtg cgcccgcaga cggcaaacga tcagcgcgaa
     6721 cagcccaaag gtcagcgcct gatacgcaac cagcagcaaa aacagtggcg ccgcggcaat
     6781 ccacggcaaa tgaccaaagc gctggagcaa accaacgatc cagtaaaagc cgccgccatt
     6841 tgcaaaaatg cccgtcacca tgccgtaaaa aaacggattt tttgtccgcg gcaggccgtc
     6901 atcgtcgagc agcacccaca aaagcggcac catcccgatc cagcacagcg gccagatgtc
     6961 gaagtcggcg cacgacaaaa acagcagcgt ccccgatagc acgcacagaa gcagtcgacc
     7021 ccagacactc tcgacaagcg gaatgagaaa acgcggcagt gccccagagc gtcggctcac
     7081 cacgtcggga gacgacgacg gagacggatc agatggcgac ggatcagacg gcagcggagg
     7141 atcagaaggt gtggcgtttt ccgccgtttg cggcccagac atcctcggca gcatagcgaa
     7201 agtcgaggcg gtctgcctac ggttcgcagc acagacgaga aactaaagtc ccgtcgtaag
     7261 atgaagcacg cgcaccacag gtttcagcgt ttcacgtacc aagttccgca ggggcggatg
     7321 ttcagcgacg agtcgcgcga cgccagggct tatcgtgtag taactgccga taagtccgag
     7381 cccaagcggt gacttcacga ggtacttgtc gcggacctgc cgcagcacgt tgacctctgg
     7441 ttcaagctct gaaccaaacg ccgccgttgc gatgaagcag cccgtcagcg tcgtaaagtt
     7501 ggcgctcgat gtgtctactt ccgtcgtcac gatcgtaccc ggcgcaccgc agcgactgac
     7561 cggctgaacc gcgatgtaat acttggtttt gggcaaaagt ccgacgatct gcgaacccac
     7621 catcgcgcca ggcggtccca gatttgccga cggcgcaggc agtgcgcggg caaagttttc
     7681 tgcggtaatc ggcccggtgc tttggtagcg aacccgatat tccgaagcgg gtgcgccggc
     7741 aggcaccgca aagccgagcc ggaacgtggt gtcggtcact tcggaaagcg ccagcttggt
     7801 cggcggcgac ggcatatcac acatccccgg atcacacggc gacaccgaca ccttgacgcg
     7861 ccaggtcccg ttgtcgtcgg tgttcatcgc caatcgatcg gcacccgatc cgccactttg
     7921 gctgatcgtc gagtcaggcg gattgagctt gccgttttcg cccttgaaat cgccgtatcc
     7981 tgcgtagtcc ttcgtcgtcg aaatccgacc gctcttgtcg agccggaacg gcactttgta
     8041 gacgatgctc ggctgtccat acggatcttt gcccaggtga tcccactcgg cgtgcggttc
     8101 cgccatcgcg cgtcccgtcg gccagtaggc gttgaaatca ccttcgatac caacctcgac
     8161 gtaaagcacg tagtcatcgt cgggaatctg cgcgtcagca aaccagcgaa tcggatcgat
     8221 cagcttgccg ttggccgggg tcgcgcccga gatcgctgcg acgtcattgt ccatcgcgaa
     8281 gcgctttaca tcggggctgt cattgcctcc caccgtcgac aagtcggcgc gcggtggata
     8341 aaccgacgtc ttgccagcgg cgtagtaacc cttgctgcca taaaacgccg acgcgcagct
     8401 tacaacgtcg acgccgttca tcttttgagt gcgccaaccc gacggtgagc agtagtaatt
     8461 ttctgtcgat gaaaccggac cgtgatacgc cacggtgttg tcgtcgtcag acggtgaacc
     8521 ctggaaatcc ggcggacagg ttggcgaaca tttgccgccc atgctgataa aaggataggt
     8581 cttgccgcgt cggtaagccc acacaggcag gctctgcggg cgacgaccat atggccagcg
     8641 aaaatcgctc ttgagccact gattgccagg ccggttgcca agccccagca tgcctatcga
     8701 tcgcgtcacg aacagatcgg tcaaaaagct gccgtcattt ttttcgatcc agacggcgat
     8761 ctgcggacgc agctcctcgc ggccattttt cggcggcatt ccctgcggcg taaagcgaac
     8821 ctcgacgcca cgacaggtcg gctcggcgtg gacagacggc gactgcgcaa tcagaagacc
     8881 caggaccgac gcacaaagtg gtcgcttcat aaggtcagta taggctggct tcgcgttggc
     8941 tttcatcgcc ccactccttg tgcttctctg cgaagaatca cgctctcgaa tggacgaagc
     9001 cgcagcgtcg atcctgctgt ctgcggcgcg tcgggatggg aagtaaacag aacatggtgc
     9061 ggctgcggaa tcgacacttc gcgccgagca tcggaaaaat tgagcaacac atccagccga
     9121 tcgtcgccat gttcgcgccg atacccgagc acatcgcgcg gcaggttcgt cggatgccac
     9181 agcacaaggc gaccggcatg cagcgctggg gttttctgtc gcagctgaaa cagccgccgg
     9241 taatgcgcaa gcagcgactg cggatcgtga agctgggctg cgacgttggt ccacggataa
     9301 cttggatgca ccggcagcca cggacgcttc cccggcggcg aaaaaccggc gttgtcatcg
     9361 gcagtccact gcatcggggt ccgacactca tcgcggttta tcagcacgcc ttttcgtcgc
     9421 agcagccgct gaagccacgg cggcacaaag cgataaaagt gcgccagcgg atcgagcgcc
     9481 gtcgcgattg gcaaatcgag gttggtcatc ccgatctctt cgccttggta gacaaacggc
     9541 accccgcgcg ccgaaagctg cagcgtcgcc agcaccttgg ctcgtcgcgg atcgtcgcca
     9601 acccgttcga tgtagcgcgc ccgatcgtga tttccgagca cccacgtcgg caagagcgga
     9661 tcggcaaagt cgcgttcgta ttcttcgatg agcgcgtaaa aagccgcggc ggaaaactcg
     9721 gtgcgcagcg tcttgaacaa aaaaaccagg ttgagcccgt cggcatgctg tccgcagtag
     9781 gatcgcagaa ggctcggtgg accaaacact tccccgacga gaaaacgcgg cccgcctggc
     9841 agtgaatcga caaacgcacg aagcgtccgc gcaaacgcaa tgctgtcggg atggttgatg
     9901 gtgtacaggt tgcgctgaaa aaaaccgtcg gcattgtctt cgcttggcaa agggcgcagc
     9961 gacggcggat tgtccaaaaa acgatcgtct ttgtagagcg cgttgaaaat atcgagccgc
    10021 agcccatcga cgcccagccg cacaaagcgc tcgatgatgt caaacatcgc tcgctgcacc
    10081 gtcggattgc ggtggttcag gtcgggctga aacggcagaa agctcgcaaa ataatagtcg
    10141 ccgctgtcgc cgtcacgatg ccaaccgcgt ggcccgatca tcgatcgcca gttcgtcggt
    10201 ggctggtttt tgcccggtcc ttttcctgga cggaacaggt agtaatcccg ctcggggctg
    10261 tctttgctgc gcagcgcggc acggaaccac ggatgctgat ccgacgtatg gtttaacacc
    10321 atgtcgatca caatccgcat tcctcgacgg tgaacttcgg aaatcagcgt ctcgaaatcg
    10381 gcccaggtcc cgtggtccgc cgacacgcgg gcatacgagc tgatgtcata gccaaagtca
    10441 cgctgcggac tctcaaagaa cggacttaac cacagcgttt ccaccccaag cgaagacatg
    10501 tggtcgagct ttgccaagat gccgggcaga tcgccaatcc catcaccgtt tgcgtcgaaa
    10561 aacgaccgcg gataaatctg ataaaccgtc gtcttttgcc accacgccgt gggatcgtgc
    10621 gcgcgctcca tctgccgctc ctcgctctat cttcgctcag tcgagggggg gaggcggctg
    10681 attcgggcga ctgcgcggaa acttcgcccg gcccgtcggt ttttcgtcaa acctgcgtgg
    10741 cgccggcctt tcatccgtcc tgcgcggcgc gggcttgtct tcaaaccgtc gcggtgcggg
    10801 cttgtcttcg tatttccgag ccggtggcct gtcgtcaaac cgtcgcggcg cgggcttgtc
    10861 ttcgtatttc cgagccggtg gcctgtcgtc aaaccgtcgc ggtgcgggct tgtcttcgta
    10921 tttccgagcc ggtggcctgt cgt
//
LOCUS       DAORGN010000172        10824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2569_length_10824_cov_5.875662, whole genome shotgun sequence.
ACCESSION   DAORGN010000172 DAORGN010000000
VERSION     DAORGN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_10824_cov_5.875662"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PKL17_16415"
     CDS             complement(<1..1195)
                     /locus_tag="PKL17_16415"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK46368.1"
                     /translation="MADVTLTDLLLGESPRIEWKQSVDAVDIIHAVCALANDLEDSQQ
                     PGYVVLGVDKLGKIVGATDKRKAPLPLFGPAMDEQQQALTSRILSLKLLPHPSLTISA
                     HEAEGKTLLVIAVEPYPVPPEVRVDGVAYVRVGTTTRRATEADLLKLRERRPLHRQPF
                     DVRPMPGAQLSDLTLIDLSPRYESDREEADDPETFPSLEKWLSQKNLGRIVDGRLKPN
                     AAALLVYGKNPQDFFPGAVIELARYAGTEVDAPVTLRKSISGTLPEQLEQVNAQLDAQ
                     LVSRPVGEPGIRAIYREEYPTKALRELARNLVQHRIYEGTHAPSRIEWYDDRVEFVNP
                     GGPFGRASEGEFGSHSDYRNPTITQLLAESGYVEQLGRGVRMVRAQLAKNGNPPLEVE
                     QNGFTQ"
     gene            complement(1242..1715)
                     /locus_tag="PKL17_16420"
     CDS             complement(1242..1715)
                     /locus_tag="PKL17_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016852495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNK46369.1"
                     /translation="MSSDPRSVAEAFYSAFTRRDGAAMAELYAPDATFSDPAFPGLRG
                     AEVGSMWKMLTGRSKDTRIEYKVVEVKGDVAIVDWQAWYTFSATGKSVHNIIRGELTV
                     KDGKIVTHVDTFDLYRWMSQAMGIKGKLLGWLPPVQKALQKQARAGLVAYMNQNK"
     gene            1865..2188
                     /locus_tag="PKL17_16425"
     CDS             1865..2188
                     /locus_tag="PKL17_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46370.1"
                     /translation="MVDILVPLIVFMTPVLLYFTRNYFRLKEKQLELAAQGGKLLPGV
                     SEESTRRIKELEERVQNLESIVIALDGERGLLPGRSQGNLAAGAESAAKLLADKSAVK
                     SSANE"
     gene            complement(2216..2923)
                     /gene="queC"
                     /locus_tag="PKL17_16430"
     CDS             complement(2216..2923)
                     /gene="queC"
                     /locus_tag="PKL17_16430"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNK46371.1"
                     /translation="MKTSQPPKAVVLLSGGLDSATVLAIVKSRGFVPYALSFRYGQRH
                     AEEIAAAQTLARHFGVAGHVIADIDLRIFGGSALTSDMPVPKDRSEAEIGGGVPITYV
                     PARNTIFLSYALAYAEVLKARDIFLGINALDYSGYPDCRPEYLAAFQQLALLATRAGI
                     EGQGITLHAPLLHMSKAEIISLGLRLGVDYGITRSCYDPTPSGEACGACDACQLRRAG
                     FRKNGLDDPAPYKISAA"
     gene            complement(2950..4425)
                     /locus_tag="PKL17_16435"
     CDS             complement(2950..4425)
                     /locus_tag="PKL17_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNK46372.1"
                     /translation="MSHPLICNFISGQWREAASGQKTANHSPATGELLGEVTASGVED
                     VEAAVASARAALPAWRSLPAPKRGEILWRAADILRRRKDEVARALTQEEGKSLTDAGG
                     EVHRAANCLEFAAGEGRRMHGQVIPSELPNNMIYTRRIPLGVVALVTPWNFPVAIPVW
                     KAAAALICGNTVVLKPASATPWCAKLITDIFIEAGMPAGVWNVLFGSGGKVGDALTQH
                     PHVEAVSFTGSNDIGQRLYSMGAASKKKIQCEMGGKNAIIVLDDADLELAAAATVQGA
                     FYSTGQRCTATSRAVVMKNVAARYRELVVEKTRALKVGNGLEPGVNVGPVVDEKQLAT
                     VLRYIEIGKQEATLLQGGERLSGALSSGLFVAPTVFSDVRPSHVIFKEEIFGPVLSII
                     EVEDETEAFAVANDSRYGLSSSVYTRDIGRAFRYIDQIETGILHVNSATVGGEAQVPF
                     GGLKDTGVGGREQGTTMLDFFTEWQSIYVDYTGTRRTASFY"
     gene            complement(4632..4865)
                     /gene="rpsU"
                     /locus_tag="PKL17_16440"
     CDS             complement(4632..4865)
                     /gene="rpsU"
                     /locus_tag="PKL17_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNK46373.1"
                     /translation="MPALIGKPLEVEVRDNLEKAMKILKQKMSKEGILQEVKRRRFYE
                     KPSVKKKRKMREARKRRRREMKRRGSGGAAPTR"
     gene            complement(5033..5635)
                     /locus_tag="PKL17_16445"
     CDS             complement(5033..5635)
                     /locus_tag="PKL17_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46374.1"
                     /translation="MFRRVEQTGSRSDIGLYLGMVLHKLGRHGEALAAFRQARRAGVQ
                     EPIADYYDAVSCFRMGLYQRARSGFAQLLSATDAGPAPQLGPRLREGAQKFAVAIEKL
                     APQTPEPTHPEAQPTRRFDAALRQAQGFLGKNNIDCQEWLEEAAALLPLLPDSERDWA
                     LTRLQDAIRQFITHLQSRPLPHPGEDLTTLQQQLKTLRSR"
     gene            complement(5812..6369)
                     /locus_tag="PKL17_16450"
     CDS             complement(5812..6369)
                     /locus_tag="PKL17_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46375.1"
                     /translation="MRMHRGSVLGAMLAFLFAPAAVLADAGPAPLVELRAAAEAQADI
                     DPDAPTWPRKEGVLLRLPHEASAVTKAESLTGASHTKLASEVSRVAIKEAIRGAVHEA
                     VAREVADRPRLALGPSSGKGTPKANETAENDRSDSGQSRAAAAQSQQARQNQAVSQAN
                     RMARGQSEKLMSPTIPGPGKTKALP"
     gene            6699..8021
                     /locus_tag="PKL17_16455"
     CDS             6699..8021
                     /locus_tag="PKL17_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46376.1"
                     /translation="MMQMSCRLIAWLLAMAVVWAGSDAVADEALSPQTALQDALPLFR
                     AQKYAQAIPLLKTASQDAELRNEVVLLLGISLLRTGALGQAEPLLREAATSGDAETKE
                     AATLFLGLLYDEQGATDQAQEQLGKASGSLSFGSAAQALRKQRRPHRLLLSLMVAPDF
                     DGNVRLTDTANWKSAPGSAADGDILFLASLGVRPFSFGLGFGNVISYRQQITLRDYSL
                     LLDTTWLGYSYSGSRNRLRVQTLFNVAMLGSSLLFLDFSGRFYDRVKLWSQLGLAGSY
                     EFRQRNYQQPDYQSLSGQTHTAQLELSYATAPDPLMFSIGYQMVRDALEQPLPPLDVS
                     DDFRAWSHGPFAKLRARLNPRVEWSLTSQYLRRKFDYVPGSELPSAQGIPRADHFFTI
                     DTSLSVRFAGYFETFFGGSMTYNQSNRDPFSYLKPTAYVGIAAYFGLL"
     gene            8084..9682
                     /gene="nadB"
                     /locus_tag="PKL17_16460"
     CDS             8084..9682
                     /gene="nadB"
                     /locus_tag="PKL17_16460"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNK46377.1"
                     /translation="MSSLVQTDYLVIGSGIAGLTFALHAAEHGEVLVVTKRQPDDGST
                     AWAQGGIAAVLDPNDSLAAHIEDTLTVGDGLCHKDIVELVVSEGPRAIRELCDSWGVQ
                     FDRAPTGELELAREGGHSARRVAHAKDTTGKEIERALLLAAQRHPRIRILANHMAVDL
                     LTLAKYGGPDSCFGAYVLSPSRHEVDVILARATVLSTGGAGKVYLYTSNPDVATADGV
                     AMAYRAGAKLANMEFIQFHPTCLFHPDAKSALVSEALRGEGGVLRLSTGESFMERYHP
                     MKSLAPRDVVARAIDAELKRSGADYVTLDMTHLPKDFLVERFPAIHAQCMQFGIDMRV
                     RPIPVVPAAHYSCGGVLTDSYGRTSVRNLYAIGETAMTGLHGACRLASNSLLEGVVFG
                     RRAADAVKETQALFPQKVIPWQSGNAQDPDEAVVVTQNWDEVRRLMWNYVGIVRTDRR
                     LLRARRRIELIREEIRDYYWNFKVTTDLLELRNLALTAHLIVESAARRKESRGLHYNT
                     DHPSRDPLYQTDTILHKEGGPSPR"
     gene            9726..10763
                     /locus_tag="PKL17_16465"
     CDS             9726..10763
                     /locus_tag="PKL17_16465"
                     /inference="COORDINATES: protein motif:HMM:NF019077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HNK46378.1"
                     /translation="MSRKGYVLGGGGFVSEGTQHYVLSLVNLDADAPRADVIPLSFLG
                     HGISFHPTDRFRAAVFEKKGPGACLVDLAARQVVRPIVSPAERRFYGHGAYSADGCLL
                     YAAESLIDKDFAGVLSVRDVESLRELGTVPTFGCSPHDCILIDEGKTLVVSNGGGPLE
                     GGDPPCVCYIDITSGKLLDKVVLDSPLFNTGHIALSTRGDLAIISAPREGLPSPNEQL
                     GALSIRPLGEDVLTVKKPGSVVQRMRGETLSLSIVEAAQLVIATNPFGNLVSMWNLRD
                     GTYVGKIDLEGPRGVCQSLDKDWFLISHVEDKSVALTAYSCGNRKPTGWALNPSFMSG
                     SHIVCHDLGPQ"
BASE COUNT         1935 a         3060 c         3568 g         2261 t
ORIGIN      
        1 cctgggtgaa gccgttttgc tcgacttcga gcggtgggtt gccgtttttg gcaagctggg
       61 cccgcaccat ccggacccct cgtccgagct gctcgacgta gccactttca gccagaagct
      121 gggtaatggt tggattccgg taatccgagt ggctaccaaa ctcgccttcg ctggcgcggc
      181 caaacggacc gcccggattt acgaactcga cacgatcgtc gtaccactcg atgcggcttg
      241 gcgcatgggt gccttcgtag atgcggtgct gtaccaggtt gcgggccagc tcacgcaggg
      301 cttttgtcgg gtattcttcg cggtagatgg cgcggatgcc gggctcgccg accggacggc
      361 tcacaagctg ggcatcgagc tgggcattta cctgctcaag ctgttcgggc agcgtcccgc
      421 tgatgctctt gcgcagcgtg actggcgcgt cgacttccgt cccggcatag cgagccagct
      481 cgatcaccgc gccaggaaaa aagtcctgcg ggtttttgcc gtagaccaag agcgctgcgg
      541 catttggctt tagtcgtcca tcgacgatcc ggcccaagtt cttttgactc agccactttt
      601 ccaggctggg gaatgtctcc ggatcgtcgg cttcctcgcg gtcggattcg tagcgcggcg
      661 acagatcaat cagcgtcaga tcagaaagct gcgctcctgg catcgggcgc acatcgaacg
      721 gctggcgatg gagaggccgt cgttctcgca gcttgagcag atccgcttcc gtcgctcggc
      781 gggtggtcgt tccgacacgg acataagcga ccccatcgac acggacttcg ggcggaaccg
      841 gatagggctc caccgcaatc acaagcagag tcttgccctc ggcttcgtgc gccgaaatgg
      901 tcagcgatgg gtgcggcagc agcttcagcg acagaatgcg gctcgtcagc gcctgctgtt
      961 gttcgtccat cgcggggccg aaaaggggca gcggggcttt tcgcttgtcg gtcgcgccca
     1021 cgattttgcc cagcttgtcg acgcccaaga ctacgtatcc gggctgctgg ctgtcttcca
     1081 gatcattggc tagtgcacag acggcgtgga tgatatcgac ggcatccacg ctctgtttcc
     1141 actcgatgcg cgggctctcg cctaagagga gatccgtcag agtcacatct gccatggccg
     1201 gcatgatagg caccgacccg gtgggtgtcc acgttctgcg gctacttgtt ttggttcatg
     1261 taggcgacga ggccggcgcg ggcttgtttt tgcagtgcct tttgtacggg aggcagccag
     1321 ccgaggagct tgcctttgat tcccatcgcc tggctcatcc agcggtagag gtcgaacgtg
     1381 tcgacgtggg tgacaatctt gccgtccttt acggtcagct ccccgcggat gatgttgtga
     1441 acgcttttgc cagtcgctga aaacgtgtac caggcttgcc agtcgacgat ggcgacatcc
     1501 cctttgactt cgacgacctt gtattcgatg cgggtgtcct tgctgcgccc ggtcagcatc
     1561 ttccacatcg atccgacttc ggcaccgcgc aggccgggaa acgctggatc agaaaaggtc
     1621 gcatctggtg catacagctc ggccatcgcc gcgccatcac gacgggtaaa tgcgctgtag
     1681 aaagcctcgg caacactgcg gggatcagaa gacatggttg acgctccttg ggcgaaagaa
     1741 ccagggcaaa aggtttcagt ccgctccatc gtatccgcaa gtgcgcaagc tttcgccaga
     1801 agctgcatgt cagtcgaacc gcgcggtaac tggcggactg gaatagctcc ggtagcatcg
     1861 ctgcatggtc gacatcttgg tcccgctgat cgtgtttatg acgccggtgc ttctgtactt
     1921 tacgcggaac tatttccgtc tgaaggaaaa gcagctagag ctggccgcgc agggaggcaa
     1981 gcttttgccg ggagtgagcg aggaaagcac ccgccgcatc aaagaactgg aggagcgggt
     2041 gcagaacctg gagtcgattg tgatagcgct cgacggcgag cgagggttac tgcctggtcg
     2101 aagccagggg aatcttgccg cgggagcgga gtctgcggcg aagcttctgg ccgacaaatc
     2161 ggcagtgaaa agcagcgcca acgaataggg ataggcccgt ctcgcccggt gcgggctagg
     2221 cagcgctgat cttgtagggg gctggatcgt cgagaccgtt tttgcggaag ccggcgcggc
     2281 gtagctgaca ggcgtcacac gcgccgcagg cttcccccga tggggtgggg tcgtagcagg
     2341 agcgggtgat gccgtagtcg acaccgagcc gcagaccgag cgagatgatc tcggccttgc
     2401 tcatatgcaa aagcggcgca tggagggtga tgccctgtcc ctcgatgccg gcgcgggtcg
     2461 ccaggagtgc aagctgctga aacgccgcca ggtactcggg ccgacagtcg ggatagccac
     2521 tgtagtcgag cgcatttatg ccaaggaaga tgtcgcgggc ctttagaact tcggcgtagg
     2581 cgagcgcata cgacaaaaag atggtgttgc gcgccgggac gtaggtgatc ggaacgccac
     2641 cgccgatctc ggcctcgctg cgatctttgg gcaccggcat gtcggaagta agcgccgagc
     2701 cgccaaagat ccgcagatcg atgtcggcaa tgacatggcc agccacgcca aagtgtctgg
     2761 ccagcgtctg agctgcggcg atttcctccg cgtgccgctg gccataacgg aaggacagcg
     2821 cgtagggcac aaagccacgg cttttgacga tggccaggac cgttgccgaa tcgagaccgc
     2881 cagagagaag gacgaccgct ttggggggct ggcttgtttt catgatcgac ctgcggggcg
     2941 ctttcgcaac tagtagaacg acgcggtgcg acgggtgccg gtgtaatcga cgtagatgct
     3001 ctgccactcg gtgaaaaagt cgagcatcgt cgtcccttgc tcgcgtccac cgacgccggt
     3061 gtccttcagt ccaccgaacg gaacctgggc ttcaccgccg accgtcgccg agttgacgtg
     3121 aaggatgccg gtttcgatct ggtcgatgta gcggaaggcg cggccaatgt cgcgggtgta
     3181 aaccgacgaa gacaagccat aacggctgtc gttggcgacg gcaaaggctt cggtctcgtc
     3241 ttcgacttca atgatcgaca gcaccgggcc aaagatttct tctttgaaga tgacgtggct
     3301 gggccggaca tcggaaaaga ccgtcggtgc gacgaacaag ccggaggaca gggcaccaga
     3361 aagccgctcg ccgccttgca aaagcgtcgc ttcttgcttg ccgatttcga tgtagcgcag
     3421 cacggtagcc agttgctttt cgtcgaccac cgggccgacg tttacgcctg gttccagacc
     3481 gttgccgact ttgagcgcgc gggtcttttc gacgaccagt tcccggtagc gggcggcgac
     3541 atttttcatc acgacggccc gcgaggttgc ggtgcagcgc tgtcccgtcg aatagaacgc
     3601 gccttgaacc gtggcggctg ccgccagttc aaggtccgca tcgtcgagca cgatgatggc
     3661 gttcttgccg cccatctcgc actggatctt tttcttgctg gcggccccca tgctgtagag
     3721 ccgctggccg atgtcgttgc tgcccgtgaa gctgacggct tcaacgtggg ggtgctgcgt
     3781 cagggcatcg ccgactttgc cgccggagcc aaagagcaca ttccagaccc ctgccggcat
     3841 gcctgcttcg atgaagatgt cggtgatgag cttggcgcac catggcgttg ccgacgctgg
     3901 cttgaggacg accgtgttgc cacagataag cgccgcggcg gctttccaca cagggatcgc
     3961 gacggggaag ttccacggcg tcaccagcgc aaccacgccg agtggaatcc gccgggtgta
     4021 gatcatgttg tttggcagct cagaagggat gacctgaccg tgcatgcggc ggccttcacc
     4081 agcggcaaac tccaagcagt tggcggcgcg atgaacttca ccgccggcat cggtcaggga
     4141 cttgccctct tcctgagtca gcgcccgtgc cacttcatct ttgcgacggc gcagaatgtc
     4201 ggcagctcgc cacaggatct cgccgcgttt gggcgctggc agactgcgcc aagctggcag
     4261 cgccgctcgc gcagaagcga cagccgcttc cacgtcttcc acgcccgacg cagtgacttc
     4321 gccaagtagc tcgccggtgg ccgggctgtg gtttgcggtc ttttgacccg aggctgcttc
     4381 gcgccactga cccgaaatga agttgcaaat gagcggatga gacatcgttt tctccgtcga
     4441 gaagagggat ggtgagtttg cgatggagcg tatgtgggcc aaaaaaccac gtcaagcggc
     4501 cttatgccct gggtcgttgc cgcagccctt tgccgacgcg ccgtcattgg caccaaatga
     4561 aaagagcccc gccaaggtga tcttgggggg ctctttggct tgccttttgg gcgtctgcga
     4621 aatctgaccg tctagcgggt gggggctgct cctccagaac cgcgacgttt catttcgcga
     4681 cgacgacgct tacgggcctc gcgcattttg cgcttctttt tgacgctggg cttctcatag
     4741 aagcggcggc gtttgacctc ttgcaggatg ccttccttcg acatcttctg cttaaggatc
     4801 ttcatggcct tttcgaggtt gtcacgaacc tcaacttcga gtggctttcc gatcaacgct
     4861 ggcacctcct ctcagcccaa attctgtttg gcgggcaaaa aagcgagact acttgagcca
     4921 ggttttggct gccacgtcaa gggtttgcgc ggcaattcaa cctgggcggt gggtcgaaac
     4981 gtccctgttt ttttggcaga tgcgacggtg tgactgtccg gtgggggggc gactagcgcg
     5041 agcgaagcgt cttcaactgc tgctgtaggg ttgtcagatc ctcgcctggg tggggcaggg
     5101 gccggctctg caggtgcgtg atgaactggc gaatcgcatc ttgcagtcgg gtcagtgccc
     5161 agtcgcgctc ggaatctggc aaaagcggca gcagcgcggc ggcttcttct agccattcct
     5221 ggcagtcgat gttgtttttg ccaagaaatc cctgcgcttg ccgcagtgct gcatcgaagc
     5281 ggcgggtcgg ctgggcttcc ggatgggtcg gctcgggggt ttgcggggcg agcttttcga
     5341 tcgcgacggc aaacttctgc gcgccttcgc gcaggcgagg gccaagctgt ggcgcaggtc
     5401 cggcgtcagt cgcggacaga agctgggcaa agccgctgcg ggcgcgctgg taaagaccca
     5461 tgcgaaagca gctcaccgca tcgtagtagt cggcaatggg ttcttgcact ccagcgcggc
     5521 gggcctgacg gaacgcggcc agggcttcgc cgtgtcgtcc cagcttgtgc agcaccatgc
     5581 ccaggtacag gccgatgtcg ctgcggctgc cggtctgttc gacacggcgg aacatggcaa
     5641 gcgcagcgtc gtactgctct gcgcggaatg accttacacc ttcgaggagg agcgtttgga
     5701 ctgctgactc ggtgctggct ggctcctgtg catgggcgac tgacgtcggc gcgaaggtgc
     5761 cggcgagcag ccacaggcca aaaacaaggc ctatgtgaga cgagtgtcgc actagggcag
     5821 cgccttggtt tttcctggac cggggattgt cgggctcatg agcttttcgc tctggcccct
     5881 ggccatcctg ttggcttggc tgacggcctg attttggcgg gcctgttgcg actgtgcggc
     5941 ggcagcacgc gattgaccgg agtcgctccg atcgttttct gcggtttcgt ttgctttcgg
     6001 tgtgcccttg ccggagcttg gtcccaaggc caggcgggga cgatctgcga cttcacgggc
     6061 gaccgcttca tggacggcgc cccggatggc ttctttgatc gcgacccgcg agacttcgct
     6121 ggccagcttg gtgtggctgg ccccggtcag actctcggcc ttggtcacgg cgctggcctc
     6181 atgcgggaga cggagcagga cgccttcctt gcgtggccac gttggggcat ccggatcgat
     6241 gtctgcttgg gcttccgctg cggcgcgtag ctcgacaagg ggagcgggtc cagcatcggc
     6301 cagaaccgcc gctggagcga aaaggaatgc cagcatggcg ccaagtaccg agccgcgatg
     6361 catgcgcatg gaaatcctca tcatctcaac cgattagtat acgggcggac accggccagc
     6421 agaaaacagc ttttggggat gaccggggat tcgccctata cccaaagaca gcaagcgctg
     6481 tgccgcctgg gtgatcttgc cgatcgacgg agatgccgcg gaaagtccgg ggcccgtgta
     6541 ccaatcggtg cgcgcagggt ccgtccgtgt actgcttggt tcggcggaga ccggggcggc
     6601 gggcccgcgt gtttggggtg ggaagacgtg gtcttggccc ggctcatctt gtgatctgcg
     6661 agcagaactt cacaatctgg ttcgcaagcg gtgctacgat gatgcagatg tcctgccgtc
     6721 tcatcgcttg gctgcttgcg atggcagtgg tttgggcagg ttctgatgcg gtggctgacg
     6781 aggcgttgtc gccgcagaca gcccttcaag atgcactgcc gctgtttcgg gcccaaaagt
     6841 atgcccaagc gataccgctg ctaaagacag ccagtcagga tgccgagctg cgaaatgagg
     6901 tggtgctgct tttgggcatc agcctgctgc gcaccggggc gcttggccaa gccgagccgc
     6961 tgctacggga agcggcgaca agtggcgatg cggaaaccaa agaggctgcg acactgtttc
     7021 ttgggctgct gtacgacgag caaggcgcga ccgatcaggc gcaagagcag cttggcaagg
     7081 cctctggctc gctgtctttt gggagtgcag cgcaggcgct tcggaagcag cgccgaccgc
     7141 atcggctgct gttgtcgctg atggtggcac ccgactttga tggcaacgtg cgactgaccg
     7201 acaccgcgaa ctggaagtca gcccccggca gtgcggctga tggtgacatc ctgtttttgg
     7261 catcgctggg tgtgcgcccg ttttcgtttg ggcttggctt tggcaacgtg atttcctacc
     7321 ggcagcagat cacgctgcgt gattacagcc tgctgctcga cacgacgtgg ctgggctatt
     7381 cctattcagg atcgcgaaat cgcctgcggg tgcagacgct gttcaatgtc gcgatgctgg
     7441 gaagcagcct gctgtttttg gactttagcg ggcggttcta tgaccgggtc aagctgtggt
     7501 cacagcttgg gcttgcggga agctacgagt ttcggcagcg caactaccag cagcccgact
     7561 atcagtcgct gtcggggcag acgcacacgg cgcagcttga gcttagctac gcgacggcac
     7621 ccgatccgct gatgttttcg attggctatc agatggtccg tgatgcgctg gaacagccgc
     7681 ttccgccact ggatgtgagc gatgacttcc gcgcgtggtc acatggaccg tttgcgaagc
     7741 tgcgggcgcg gctgaatccg cgggtcgagt ggtcgctgac ttcgcagtat ctgcggcgga
     7801 aattcgatta cgtgccgggc agcgagctgc cgagtgcgca aggaattccg cgcgccgatc
     7861 atttctttac catcgataca tcgctgtcgg tgcgcttcgc tggttacttc gagacgttct
     7921 ttggcggatc gatgacctat aaccaaagca accgcgaccc gttttcctac ctgaagccga
     7981 cggcatacgt tgggattgcg gcgtactttg gccttctgta atgcccgctg agggaatcga
     8041 ctatttttgg tgacggtgag cggaaaatcg cgatgatgcg ggcatgtctt cgctagtcca
     8101 gaccgactat ttggtcatcg gatcgggaat tgccggactc acgtttgccc tgcacgctgc
     8161 cgaacatggc gaggtcttgg tcgtcaccaa acgtcagccc gacgatggca gcacggcctg
     8221 ggcgcagggc ggtatcgcgg cggtgctcga cccaaacgac tcactggctg cgcacatcga
     8281 agacaccctg acggtcggtg atggtctgtg ccacaaagac atcgtcgagc tggtcgtttc
     8341 agaagggccg agagcgattc gcgagctgtg tgatagctgg ggtgtgcagt ttgatcgcgc
     8401 cccgactggc gagctggagc tggcgcgcga aggcggtcac tcggcgcgaa gagtggctca
     8461 tgccaaggac accacgggaa aagagatcga gcgggcgctt cttttggcgg cgcagcggca
     8521 tccgcggatt cgcattttgg cgaatcacat ggccgtcgat cttctgacgc tggccaagta
     8581 cggcggtccg gattcctgct tcggtgccta cgtgctgtca cccagtcgac atgaagtcga
     8641 tgtgatcctg gcccgcgcga cggtcttgtc gacggggggg gctggcaagg tgtatctgta
     8701 tacgtcgaat cccgatgtcg cgacggcgga cggggtcgcg atggcgtatc gggccggcgc
     8761 aaagctggcc aacatggagt ttatccagtt tcacccgacg tgcctttttc atcccgacgc
     8821 gaagtcggcg ctggtatcgg aagcgctgcg tggagaaggt ggggttctgc ggctttcgac
     8881 gggagagtcg tttatggagc gctaccatcc gatgaagtca ctggcaccgc gtgatgtggt
     8941 ggctcgggcg attgatgcgg agctaaagcg ctcgggggcg gactatgtga cgctcgacat
     9001 gacgcacctg ccgaaagact ttctggtcga gcggtttcct gccatccacg cgcagtgcat
     9061 gcagttcgga atcgacatgc gggtccgtcc gatcccggtg gttccggcgg cgcactactc
     9121 gtgcgggggc gtgctgaccg attcgtatgg tcgaacgtcg gtgcgcaacc tgtacgcgat
     9181 cggtgaaacg gcgatgacgg gactacacgg cgcctgtcgg ctggcgtcca actcgctgct
     9241 ggaaggggtg gtgtttggtc gtcgcgcggc ggatgcagtc aaagaaaccc aggcgctgtt
     9301 cccgcagaag gtgattccgt ggcagagcgg caatgcccaa gatccggatg aagcggtcgt
     9361 cgtgacccag aactgggatg aagtccgccg gctgatgtgg aactacgtcg gaattgtccg
     9421 gaccgaccgg cggcttctgc gggcgcggcg gcggatcgag ctgattcggg aagagatccg
     9481 ggactactac tggaacttca aagtgacgac cgacctgctc gagctgcgaa atttggcact
     9541 taccgcgcat cttattgtcg agtctgcggc ccggcgcaaa gagagccgcg ggctgcacta
     9601 caacaccgat catcccagca gagatccgct ctaccagacc gacaccatct tgcacaaaga
     9661 gggcggcccg tctcctcggt aacgtggtct ttacgcggcg tgggggaggg gagtatgttc
     9721 tgccgatgag ccgcaaaggg tatgtgctgg gtggtggtgg tttcgtttcg gaaggaacgc
     9781 aacactatgt gctatcgctt gtgaacctgg acgctgacgc gccgagggca gacgtgattc
     9841 cgctttcctt tttgggacac ggaatttcgt ttcatccgac cgatcggttc cgcgcggcag
     9901 tgtttgaaaa aaagggtccg ggggcgtgtc tggtcgatct cgcggcgcga caggttgtgc
     9961 gtccgattgt gagccctgcg gagaggcgct tttatggcca tggtgcgtac tcggcggatg
    10021 gctgtttgct ctatgcagcg gagtcgctga ttgacaagga cttcgctggg gtgctgtcgg
    10081 tgcgcgatgt ggaatcgctg cgcgagctgg ggaccgttcc caccttcggt tgctcgccgc
    10141 atgactgcat cttgatcgac gaaggaaaga cgctggtggt gtcaaacgga ggcggaccgc
    10201 ttgaaggtgg cgacccgccg tgtgtctgtt atatcgacat cacgtctggc aagctgctcg
    10261 acaaggtggt gctggattca cccctgttta atacgggaca catcgcgctg tcgacgcgag
    10321 gggatctggc gatcatttcg gccccgcggg aaggactacc cagtccaaat gaacagctcg
    10381 gtgcgctgtc gataaggccg cttggggaag atgtgctgac ggtcaagaag cccggatcgg
    10441 tggtccagcg gatgcgcggc gagacgctgt ccctttccat cgtggaagcg gcgcagcttg
    10501 tgattgcgac caatccgttt ggcaatctgg tttcgatgtg gaacctgcgc gacggaacct
    10561 atgttggcaa gatcgatctg gaaggaccac gcggcgtctg tcaatcgctc gacaaggact
    10621 ggtttttgat aagccatgtg gaagacaagt cggttgcgct gacagcgtat tcctgcggca
    10681 atcgcaagcc gacgggctgg gccctaaacc ccagcttcat gagcggttcc cacatcgtct
    10741 gtcacgatct gggtccgcag tgatttcgac gtccggcgtt ggctctggac gcggcgcgca
    10801 aggcttgggc aaccggggcg agga
//
LOCUS       DAORGN010000173        10823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2570_length_10823_cov_5.238856, whole genome shotgun sequence.
ACCESSION   DAORGN010000173 DAORGN010000000
VERSION     DAORGN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2570_length_10823_cov_5.238856"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1738
                     /locus_tag="PKL17_16470"
     CDS             191..1738
                     /locus_tag="PKL17_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HNK46379.1"
                     /translation="MHSGNRVVVIGSGFGGLAAAIRLQARGYQVTLLEQRDQPGGRAY
                     VYRDAGYVFDAGPTVVTAPYLFEELFALCGRKMSDYLEMLPVNPFYRVRFHDGRYFEY
                     SGNTEQMVEQIRAWNPADVDGYLRFVEESERIFKVGFEQLGDVPFHKLTDMLKIVPQM
                     LRLRSHLSVYSLVKSFIQHPDLRILMSFHPLLVGANPFKTTSIYALILYLERKWGVWF
                     AKGGTGAIVKALCDLFVEMGGEVRLHAKVEQILVEGGGLKPKASGVRLAGGATLPADL
                     VVSNAEVGFTYRYLLPEDARRHFTNERVENTNYSMGLFVAYFGTKRRFDDVAHHTIAL
                     GPRYRELLDDIFTRKTLADDFSLYLHRPTATDTSLAPPGGDAFYVLSPVPHLGADRPV
                     DWSTEGERYRDKIFSYLEQTMLPGLRDQLVTSRFITPITFRDELSSMYGSGFSVEPTL
                     LQSAYFRPHNVSEDVEGLYIVGGGTHPGAGMPGVLSTAKVVDRLIPRLASAPQGSPIA
                     GSHAAYV"
     gene            1731..2792
                     /locus_tag="PKL17_16475"
     CDS             1731..2792
                     /locus_tag="PKL17_16475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNK46380.1"
                     /translation="MSSPQPSTSLAETVPQAIAQDDLLLCQRHIAHHSKSFYLSSLLL
                     PRKVRYDAWALYAFCRQADDAVDGDNPGDGTVPNNAPGASAQLAAVANLRKKLAAVYR
                     GDPGQGTAHAIDRAFSKVVVRTAMPQALPSRLLDGMEQDARGQHYGSWEDLYGYCFNV
                     AATVGLMMTCVMGHKLSPDRLAEVLLRASDLGVAMQLTNIARDVGEDARRGRVYLPDS
                     LLGKHGLRAESVLAEAMQGSPASAGLRAAVAELLATADAHYAAAEVGIAMLPDGVQWA
                     IRSASRIYQRIGRALKAQGFDSLRKRARISALHKLVLLFGAYLLGLLPSARRISALPS
                     TGPADQLLLRLCREVKLLP"
     gene            complement(2806..3909)
                     /locus_tag="PKL17_16480"
     CDS             complement(2806..3909)
                     /locus_tag="PKL17_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46381.1"
                     /translation="MHIPAWGFASFLALLVAGCPGQTSIVQTPVVSPPSDGGSSLTSC
                     LPQRSLDLVQYLRELPFVGRDGGPMIPVSRGTTQWMSAYSTALAWASADCPSFMAYAD
                     AMGYKAEEITDTVTQTRHWVLTERASRWNGFFVLRAPSEVAKARPLTITAPHLGYDFG
                     DDRALILYRELGALGLLQNTAHRCNLATCSGCTAFAGYACSGCPRASDLAHSVDHLMF
                     ALFSGLEAMHKVVRFEYHGAAEHQSSPDCSSTVNLSQGTTEKLPPEVDDKTPASRMWK
                     ALERSLGPRCVCYHQRESACVLPGTNSVFGRLTNQEPTIPFDPCTTAAARSSGRFIHF
                     EGQSVAVERVIAALSEAVPMPTPRPLPVVPPHP"
     gene            complement(3918..6356)
                     /gene="lon"
                     /locus_tag="PKL17_16485"
     CDS             complement(3918..6356)
                     /gene="lon"
                     /locus_tag="PKL17_16485"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNK46382.1"
                     /translation="MFDPKASHSEPTPELPVLPLRNLTLLPGLTLPVEVGRRSSLRML
                     ENVVKQHPPTLLVAVQRDPHTEDPQPSDLHPIAVQAEVLKVVRTGSDKMTAILKGERR
                     RRFEVVGTEPFLTARSLPAPELLADSVETIALALTVKEAGQRLASQIQVPESARAVLQ
                     LLDQTREPSLVSDLASALVELPLAERLSLLNQLDVKVRLHQVLAVLQRQAEVMSAKER
                     IDKQVQNEFARRQREAVLRQQMKAIQDELGESDNDKDLQQFSERIAQAEMPAEAEKVA
                     KKQLDRLRNIPEGSPEASIQRTYLEWLCDLPWSKRTNDKHDLPSARAVLEADHWGLEK
                     VKKRIVEYLAVRKLAPTKKGPILCLVGPPGVGKTSLGRSIASALGRNFHRISLGGVRD
                     EAEIRGHRRTYIGALPGRIIQAMKRVGSKNPVILLDEIDKLGHDYRGDPSSALLEVLD
                     PEQNNAFSDHYLEVSFDLSEVIFICTANTLETIPLALRDRMEIIEIPGYTIAEKQKIA
                     ERHLLPKQIGEHGLKPDQIQFTETAISEIIERHTREAGVRNLERELASVIRGVAMKIA
                     EQPAPAQDAEKKAIVVDQVDVAEYLGPIKFFHDVAEHTNCPGVATGLAVTPVGGDILF
                     VEASAMPGKGKLQLTGKLGDVMKESAQAALSWVRAHSTELHVDPGFFENMDLHLHVPQ
                     GAIAKDGPSAGTAILSAIVSLLTGRKVRSDVAMTGEVTLRGAVLPVGGIKSKVLAAHR
                     AGIRTVFLPERNGKDVLEVPQDVRNEMSFVVVSNVRTVLDSVLEPTPSGAPLAPLPAS
                     SSSVQTGPAAPAMA"
     gene            6754..8664
                     /locus_tag="PKL17_16490"
     CDS             6754..8664
                     /locus_tag="PKL17_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46383.1"
                     /translation="MTIAPSLRCFECGATYPASTRTCEQDGAPLCPETVGQHWKVEGV
                     LAPRLGGGVFAAFHAATGVRAAITFFPSGSTKDQAILDRIARELTGLRILDKNRGILT
                     LIEEGSEPDGSRFLVHELGTLRLLQDMLDEWQKPDRGLLDPPVAAQLVRPLLSALSTA
                     HRIGVAHASLTPLDIYLLPEGEPAEGITRVVEARLHGLLTFPMGPELRQAVAADLRAI
                     GATLFELLTGRRPPDSVEALRALPLPKVMQSPVGKVAMRALGGTEQPVYLGAEEMLRA
                     LVTTIPTVLAPPNLDIEAATRVDPDPTKRAQPQSKKVETVNTKPTVPVEILQTPARAV
                     PSQPVLPLPAAAGPVRSGLTAELHQVSIHDLMKERELLQKQGPVETLARSPQHRSSVT
                     DMPMIPPPPDELAPPPAPPSQSSGPSKLEAPVAMPREGVFQGISFGQEPTIPAVVNAS
                     LLAASAERSQYGTVPAELVPRPAAGGQADKAATGASVVSVSGSLPKPLQTSGALVIPP
                     QPSGPVSVPSSKPPSVSASGASFEPVRLGGPSGPARVVSRPPTAPSPGLPLEYEPTPA
                     AGQPPVDPDGATEPAPALRPLAAGQGQSSTEPATKAAAPVPAGRELPPWVWAAILAVV
                     CLLALLAGLFGR"
     gene            8711..10663
                     /locus_tag="PKL17_16495"
     CDS             8711..10663
                     /locus_tag="PKL17_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46384.1"
                     /translation="MRLLWSRLVLVFALLGVGYGMAVAEPSGDGCFRGLSQDGTAGTL
                     DLPSADPGCPWRLRSGAYLGGFVEDGYVIVGDRFRSIAGGMAMAATLGRHVELGLQLG
                     AQLGRAEQIGGPLAGTTAPSLLSMSQLKVQLKLFSPWGRYVHVALLPSVRVPGTGQDF
                     VPAPLNTDVSIDGLLQVSLQPKLPFLPLRLSMLLGYAQDRSLRALDAQDCMGGTVADC
                     LRVRLESTAAYGVGLPRVRGSLGVELPLSLHRFLSITPAVIYRIEAIVGDPDPVLIAL
                     FGMGFPGAPLQSRFQQWLTIGGRLGLGIPLAIDFGVRVGLQSAGYAMGAKLPKVMGFG
                     GLSWELDLLGDHGAGGRSGSLTKAQPSPSTEPVRCRVSGTVRDAQTGQALADAVVRFV
                     GQRHNALLSDDKGGFASGPLLCGAVYIEASRGDRQTTRIPVVLAAGDSESVELRLPRR
                     ERTQSGGLWLLLRTADGTKPAVYATLSREGQQQALQGEQSGLFARVAAGAWLLRVDAA
                     GFLSREQTVFISDGGEQRVELTLQRRGAQPKVQLGQSELVLREPLVFVPASSVLSPDS
                     ERLLDEVSDLLIHHPEVALLHIEHQADLSMSDPLLLEQQVIAVRDYLVQHGTAPERLV
                     ARLVDGPRRAPAKIVLKLSAERRPAP"
     gene            10668..>10823
                     /locus_tag="PKL17_16500"
     CDS             10668..>10823
                     /locus_tag="PKL17_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNK46385.1"
                     /translation="MALEQPFPTLRIARDSDLALIVATYNATIASRRVTADLVPVTVD
                     SRRPWFAA"
BASE COUNT         1880 a         3188 c         3542 g         2213 t
ORIGIN      
        1 tgaaatgggg cccgataagc ggtgccaaac caaggtggat cttgaaatgg gcccgatgcg
       61 tggcgttttt caagacgaac cttgatgtgg atggcgcttg ctggggggct tgcaaaggca
      121 gacttttggg gcgggttttt gctgaagcgg gccgggtcgc tgtggcacct tatcgggcat
      181 gaaaacggcg atgcacagtg gcaaccgggt ggtggtgatt ggctcggggt ttggcggact
      241 tgcagcagcg attcgtcttc aggcacgcgg ctaccaagtg acactcctag agcagcgcga
      301 ccagccgggc ggacgggcct atgtctaccg cgatgctggc tacgtgttcg atgcgggccc
      361 cacggtggtc acggcgcctt atctgttcga ggagctgttt gcgctgtgtg gtcgcaagat
      421 gagcgactac ctggagatgc tgccggtcaa tccgttctat cgggtgcgtt tccacgacgg
      481 gcgctacttc gaatacagcg gcaacaccga acagatggtc gaacagattc gcgcctggaa
      541 tccggcagac gtcgacgggt atctgcgctt tgtcgaggaa tccgagcgca ttttcaaagt
      601 cggtttcgag cagcttggcg atgtgccgtt tcacaagctg accgacatgc tgaagatcgt
      661 gccgcagatg ctaaggctgc ggtcgcatct gtcggtgtat tcgctggtga agagcttcat
      721 ccagcatccg gatctgcgga ttctgatgtc gtttcacccg ctgctcgtcg gcgccaatcc
      781 attcaaaacc acgtcgatct atgcgctgat cctgtatctg gaacgcaagt ggggcgtctg
      841 gtttgccaaa ggcggcaccg gcgcgattgt caaagcgctg tgtgatctgt ttgtcgaaat
      901 gggtggcgaa gttcgcctac acgccaaagt cgaacagata ctcgtcgaag gtggcggtct
      961 caagccgaaa gccagcggcg ttcgtctggc gggaggggcc acactgccgg ctgatctggt
     1021 ggtcagcaac gcggaagtcg gctttaccta ccgatatctt ttgcctgaag acgcgcgtcg
     1081 ccacttcacc aacgaacgag tcgagaacac caactactcg atgggactgt ttgtggccta
     1141 ctttggcacc aagcggcgct ttgacgacgt ggcccaccac acgattgcgc ttgggccacg
     1201 gtatcgcgag cttctggacg acatctttac gcgcaagacg ctggccgatg acttttcgct
     1261 gtacttgcac aggccgacgg cgaccgatac ttcgctcgcg ccgccgggtg gtgatgcgtt
     1321 ctatgtgctg tcgccggttc cccacctggg ggccgatagg ccggtggact ggtcgacaga
     1381 aggcgagcgc tatcgcgaca agattttttc ctatctggaa cagacgatgc tgccgggtct
     1441 gcgcgatcag cttgtcacct cgcgtttcat cacgccgatt acgttccgcg atgagctgtc
     1501 atcgatgtac ggatcgggct tttcggtcga gccgacgctg cttcagtcag cctatttccg
     1561 tccccacaac gtgagcgaag acgtcgaagg tctttacatc gttggcggag gcacccatcc
     1621 gggcgctgga atgccgggcg ttttgtcgac ggccaaggtg gtcgaccgct tgattccccg
     1681 actggcttct gcaccgcagg gttcgccgat agccggatcg cacgcggcgt atgtctagtc
     1741 cgcagccttc gacttcactt gcggaaacag tcccgcaggc gattgcgcaa gacgatctgc
     1801 ttttgtgtca aaggcacatt gcccatcaca gcaagagctt ctatctgtcg tcgctgcttc
     1861 tgccgcgaaa ggtccgctac gatgcctggg cgctgtatgc attttgtcgc caagccgatg
     1921 atgctgtaga cggcgacaat cctggtgatg gcaccgtgcc gaacaatgcg cccggtgcgt
     1981 ctgcccagct agcggcggtc gcaaacctgc gcaaaaaact cgcggcggtg tatcgcgggg
     2041 atccggggca aggcacagcg catgcgattg accgggcgtt ttcaaaagtg gtcgtgcgga
     2101 cggcgatgcc gcaggcgctt ccttcgcggc tgctcgatgg aatggagcaa gatgcgcgcg
     2161 gccagcacta tgggagctgg gaagacctgt acggttactg cttcaacgtc gcggcgaccg
     2221 tgggactgat gatgacttgc gtgatgggtc acaagctgtc acctgacaga cttgcggaag
     2281 tgctgctgcg ggcgtcggac ctgggcgtgg cgatgcagct taccaacatc gcgcgcgacg
     2341 tgggcgaaga cgctcggcgg ggtcgggtgt atctgccgga ttcgctgctt ggcaaacatg
     2401 ggcttcgcgc cgagtcggtc ttggcggaag cgatgcaagg atcgccggcc agtgcaggct
     2461 tgcgcgctgc ggtggcggag cttttggcca ctgcggatgc ccactatgcc gctgccgaag
     2521 tcggaattgc gatgctacct gacggggtgc agtgggcgat tcgttcggcg agccggattt
     2581 atcagcggat tggacgcgcg ctcaaagccc aagggtttga ttcactgcgc aagcgggcgc
     2641 ggatttcagc actgcacaag cttgtgcttc tgtttggcgc gtaccttctg ggacttttgc
     2701 cgtcggcgcg acggatttct gcgcttccgt ctacgggacc ggctgaccag ttgcttttgc
     2761 gtctgtgtcg tgaagtgaag ctgcttccgt aacgccggac ttgggttacg gatgcggcgg
     2821 cacgactggc agcgggcgcg gcgttggcat cggcactgct tccgacagtg cggcgatgac
     2881 ccgttcgacc gctacgctct gtccttcaaa gtggatgaac cgacccgagc tgcgtgctgc
     2941 cgccgtcgtg cacggatcaa agggaatcgt cggctcttgg tttgtcagcc gtccgaagac
     3001 cgagtttgtc cctggcagga cgcacgcaga ctcgcgctgg tgataacaga cgcagcgcgg
     3061 accgagcgac cgctctagtg ctttccacat ccgacttgcg ggggttttgt catcgacttc
     3121 ggggggcaac ttttcggtgg tcccctgcga cagattcacc gtcgagctgc aatccggact
     3181 ggactgatgt tcggcagcgc cgtggtattc gaaacgcacc actttgtgca tcgcctctag
     3241 gccggaaaac agcgcaaaca tcaggtggtc gaccgagtgc gcgagatcgg aagcgcgcgg
     3301 gcagcccgag caggcatagc ctgcaaatgc ggtacagccc gagcaggtcg ccagattgca
     3361 gcggtgcgcg gtgttttgca aaagcccaag cgcgcccagc tcgcgataca aaatcagtgc
     3421 ccgatcgtcg ccaaagtcgt aacccaggtg cggtgccgtg atggtcagcg ggcgggcttt
     3481 ggccacttcc gatggcgcgc gcaggacaaa gaagccattc cagcggctgg cgcgctcggt
     3541 caaaacccag tgcctagtct gggtcacggt gtcggtgatt tcttcggctt tgtaacccat
     3601 cgcgtcggcg taggccataa agctgggaca gtcggcagac gcccaagcca gcgcggtgga
     3661 atacgccgac atccactgcg tggtaccgcg cgacaccgga atcatcgggc caccatcgcg
     3721 accgacaaaa ggcagctcgc gcaggtactg cacgagatcc agcgagcgct gcggcagaca
     3781 actggtcaag ctgctgccgc catcactcgg tggactgacg acgggggtct gaacgatgct
     3841 cgtctggccc ggacagccag ccacaagaag tgccaaaaac gaagcgaagc cccaggcggg
     3901 gatgtgcatg ccttcggcta tgccatggcg ggcgcggcgg gtccagtctg gacgctgctt
     3961 gatgaagcgg gcaggggagc cagcggcgct ccgctcggcg tcggttcaag cacgctgtcg
     4021 agcacggtgc ggacgttgct gacgacgaca aacgacatct cgtttcgcac gtcttgtggg
     4081 acttccagca cgtctttgcc attgcgttcg ggcaaaaaca cggtgcggat gccagcgcgg
     4141 tgagcggcca gcactttgga cttgattcca ccgacgggca gaaccgcgcc acgcagcgtc
     4201 acttcgcccg tcattgcgac atcagaccgc actttgcgcc cggtcagaag cgacacaatg
     4261 gccgacagaa tcgcagtgcc cgccgacgga ccatccttgg caatcgcgcc ttgcggtacg
     4321 tgcaggtgca gatccatgtt ttcgaaaaag ccggggtcga catgcagctc cgtcgagtga
     4381 gcgcgaaccc acgacagcgc cgcttgcgcc gattccttca tgacgtcgcc gagcttgccg
     4441 gtaagctgga gcttgccttt gccgggcatg gccgatgctt cgacaaacaa gatgtcgcca
     4501 ccaaccggag tcaccgcaag ccccgtcgcg acgcctgggc agttggtgtg ctctgcgaca
     4561 tcgtggaaga acttgatcgg gcccaggtat tctgcgacat caacctggtc gacgacgatg
     4621 gccttctttt cggcatcttg ggccggcgcc ggctgctcgg caatcttcat cgcgacgccg
     4681 cggatcaccg acgccagctc tcgctcgagg ttgcgcacgc cggcttcgcg ggtgtgacgc
     4741 tcgatgattt cgcttatcgc cgtctcggta aactggatct gatccggctt gagaccatgt
     4801 tcgccaatct gcttgggcag aaggtgacgc tcggcgatct tctgtttttc cgcgatggtg
     4861 tagccgggga tctcgatgat ctccatgcgg tcacgcagcg cgagcggaat cgtttccagc
     4921 gtgttcgcgg tgcagatgaa gatcacctcc gacagatcaa acgacacttc caggtagtga
     4981 tcggaaaagg cgttgttctg ttcgggatcg agcacttcca gaagcgcgct cgatggatcg
     5041 ccgcggtagt catgacccag cttgtcaatc tcatcgagga ggatgaccgg gtttttgcta
     5101 ccgacgcgct tcatcgcttg gatgatgcga ccgggcagcg cgccgatgta cgtccgccgg
     5161 tggccacgga tttcggcttc gtcccgcact ccgccaagcg agatgcggtg gaagttccga
     5221 cccagcgccg atgcaatcga gcgaccaagc gaggtcttgc caacacccgg cggaccgacg
     5281 aggcacagaa tcgggccctt cttggtcggc gccagcttgc gaaccgccag gtattcgacg
     5341 atccgttttt tgactttttc aagaccccag tgatcggctt ccagcaccgc gcgcgccgac
     5401 ggcagatcgt gtttgtcgtt cgttcgcttc gaccagggca gatcacacag ccattccaga
     5461 taggtgcgct gaatcgacgc ttcgggcgag ccttccggaa tgttgcgcag gcggtcgagc
     5521 tgctttttcg cgaccttttc cgcttcggct ggcatttcgg cctgggcaat ccgttcggaa
     5581 aactgctgca ggtctttgtc gttgtcgctt tcgccgagct cgtcctgaat tgccttcatc
     5641 tgctggcgca gtacggcttc gcgctggcga cgggcaaact cattttggac ctgcttgtcg
     5701 atgcgctctt tggcgctcat cacttcggcc tgtctttgca gcaccgccag gacctgatgc
     5761 agacggactt tgacatcaag ctggtttagc aaactcagtc tttcggccag cggcagctcg
     5821 acgagcgccg aggccaaatc gctgaccaag ctgggctcgc gggtctgatc gagaagctga
     5881 agcactgccc gcgctgattc gggaacctgg atctgcgaag ccagccgctg tccggcttct
     5941 ttcaccgtca gcgcaagcgc aattgtttcg accgaatcag ccagaagctc gggcgccggc
     6001 agcgagcgtg ccgtcaaaaa cggctccgtg ccaacgacct caaagcgacg acgacgctcg
     6061 cctttgagaa tcgccgtcat tttgtctgat cccgtgcgca ccactttgag cacttcggcc
     6121 tgcaccgcaa tcgggtgaag gtcagaaggc tggggatctt cggtgtgcgg gtcgcgctgg
     6181 accgcgacaa gcagcgtcgg gggatgctgc ttgacgacgt tttccaacat acgcagcgag
     6241 ctgcgacgac ccacctcgac agggagagtg aggccgggca aaagtgtcag gttgcgcagc
     6301 ggtaggaccg gaagctcggg cgtcggttcg ctgtggcttg ctttggggtc aaacatggtt
     6361 tcctctgggt tcagacctcg tcgctaaccg acaggtccgg cgcaataaca gcctcaatct
     6421 gcgcacgcac ttggtgagaa gcaatactcc aattttacga tgccttggcg gcggagcttg
     6481 catcttctca cgaaacaccg agactgcctc ggtgcggcgc gcgatatggg caaactaagg
     6541 gcgcgctgcc ggctgtcaat gtgtgtcttg tgcaaaagtt tccctaacgt cacttttggc
     6601 tgatggctgg gagtcagctg cgcccgctcc gtcgaattcg ccgtcgcaac cgttcacgat
     6661 ggcatcgcac aggccgcgct ggaccggcaa acttcaccga agacgagccg acttgtcgtg
     6721 ataaagtgat caaaccgccg tcgaaggttt gccatgacaa tagctccctc tctgcgctgc
     6781 tttgagtgcg gcgcaaccta tcccgcctcg acgcgcacct gtgagcaaga tggtgcgcca
     6841 ctatgtcccg agacggtagg tcagcactgg aaagtcgaag gcgtgctcgc gccccggctt
     6901 ggcggcggtg tgttcgctgc gttccatgcg gccaccggag ttcgcgccgc catcacgttt
     6961 ttcccatcgg gaagcaccaa ggatcaggcc atcttggacc gcatcgcccg cgagcttacg
     7021 gggctgcgca tcctcgacaa aaatcgcggc atcctgactt tgatcgaaga aggcagtgaa
     7081 cccgacgggt cacgctttct ggtccacgag ctggggacgc tgcggctctt gcaagacatg
     7141 ctggatgaat ggcaaaagcc ggatcgtggc ctgctggacc cgccggtggc ggcgcagctg
     7201 gtgcgaccgc ttttgtcggc tttgtcgacg gcacatcgca tcggtgtcgc ccacgccagc
     7261 ctgacacctt tagatatata tctgcttcca gaaggcgagc cggcagaagg catcacgcgc
     7321 gtcgtcgaag cgcggctgca tggtctgctc acgtttccga tggggcccga gctgcggcaa
     7381 gcggtcgcgg cggatctgcg ggcgattggc gcgacgctgt ttgagctttt gacgggtcgt
     7441 cggcctcccg attccgtcga agcgctgcgg gcgctgccgc tgccgaaagt gatgcagtcg
     7501 ccggttggaa aagtcgcgat gcgggcgctt ggtgggaccg agcagccggt ctacctgggc
     7561 gccgaggaaa tgttgcgcgc gctggtgacg acgattccga cggtgctggc tccgcccaac
     7621 ttggatattg aagcggcgac gcgggtcgat cctgatccga cgaagcgcgc gcaaccgcag
     7681 agcaagaaag tcgaaacggt caacaccaag ccgacggtgc cggtcgaaat tttgcagact
     7741 cccgcgcggg cggtgccgtc gcagccggtg ttgccgcttc ccgcggcagc ggggccggtt
     7801 cgcagcgggc ttaccgccga gctgcaccag gtgtcgatcc acgatctaat gaaagagcgc
     7861 gaacttctgc aaaagcaggg gccagtcgag acgctggctc gttcgccaca gcatcgatcg
     7921 tcggtgaccg acatgccgat gattccgccg ccgcccgatg agcttgcgcc accacctgcg
     7981 ccaccgtcac aaagctcggg acccagcaag ctggaagccc cggtggcgat gccgcgcgaa
     8041 ggcgtgttcc aggggatttc gttcggtcag gaaccgacga ttccggcggt ggtcaatgcg
     8101 tcgcttttgg cggcttcagc agagcgttcg caatacggca cggtcccggc agagctggtg
     8161 cctcgtccgg cagccggtgg tcaggccgac aaagcggcga caggcgcatc ggtggtttcg
     8221 gtcagcggct cgctgccgaa accgcttcag acgagcggag cgctggtgat tccgccgcag
     8281 ccatcgggtc cggtcagcgt tccatctagc aaaccgccat ccgtgagcgc gagcggtgcg
     8341 tcgtttgagc cggttcgtct gggcggacca tcgggtccgg cgcgcgtggt gtcgcgtccg
     8401 ccgaccgcgc catcgcctgg tctgccgctg gaatacgaac cgacgcctgc ggcaggtcag
     8461 ccgcccgtcg atccagatgg tgcgaccgaa ccggccccgg cactgcgtcc ccttgcggcg
     8521 ggtcagggcc aaagctcgac ggaaccagca acaaaggcgg cggcgccggt ccctgcgggg
     8581 cgagaactgc caccgtgggt gtgggcggcc atcttggcgg tggtctgtct gctcgcgctg
     8641 ctcgccggac tgtttggtcg ctagctgctg caaaccggct gcgactccgt gctatgtagg
     8701 ccttattccg atgcgccttc tgtggtcccg ccttgtgctc gtgttcgcgc tgcttggcgt
     8761 gggatacggc atggccgtcg cagaacctag cggtgatggc tgttttcgcg gcctaagcca
     8821 agacggaact gccggcacgc tggatctgcc aagcgctgat ccgggctgtc cctggcggct
     8881 gcgcagcggt gcgtatttgg gcggtttcgt cgaagacggt tacgtcatcg tgggtgatcg
     8941 ctttcgctcg attgcgggcg gcatggcgat ggcggccacg ctcggtcggc atgtcgaact
     9001 gggacttcag ctcggggcgc agctcggacg ggccgagcag atcgggggtc cgctggcggg
     9061 cacgacggcg ccatcgcttt tgtcgatgtc gcagctgaaa gtgcagctca agctgttttc
     9121 gccgtggggc cgctacgttc atgttgcgct tttgccgtcg gtccgcgtgc cggggactgg
     9181 ccaagacttt gtgccagcac cgctcaatac cgatgtgtcg atagatgggc tgctgcaggt
     9241 ttccctacag cccaagctgc cgtttttgcc gctgcgcctg tcgatgctgc ttggctacgc
     9301 gcaggaccgc agcttgcgtg cgctggatgc ccaggactgt atgggaggca ccgtcgctga
     9361 ctgcctgcga gtgcggctgg aaagcacggc agcttatggc gtcggcttgc cgcgcgtgcg
     9421 tggttcgctg ggcgtcgagt tgccgctttc actgcatcgg tttttgtcga tcacaccggc
     9481 tgtgatctac cgaatcgagg cgattgttgg ggatcccgat ccggtgctga tcgcgctgtt
     9541 tgggatgggg tttccggggg cgccgctcca gagtcgcttt cagcagtggt tgaccatcgg
     9601 cggtcgcttg ggcctgggaa ttccgctggc gatcgatttt ggcgtgcggg tcgggctgca
     9661 aagtgctggc tacgcgatgg gcgccaagct acccaaagtg atgggattcg gcggtctgtc
     9721 gtgggaactg gacttgctcg gtgaccatgg cgcgggcggg cgcagcgggt ctttgacgaa
     9781 agcccagccc agtccgtcga cggaacccgt gcgatgtcgg gtgtccggta cggtccgcga
     9841 tgcccagacg ggacaggcgt tggccgatgc ggtggttcgt tttgtcggac agcgtcacaa
     9901 tgcgcttctc agcgatgaca aaggtgggtt tgcgagcggt ccccttttgt gcggcgcggt
     9961 ctacattgaa gccagtcgcg gcgataggca gacgacgcgg attcccgtgg tgctggccgc
    10021 tggggattcg gaatcggtcg agcttcgtct gccgcggcgt gagcggacac agagcggggg
    10081 tctgtggctt cttttgcgga ccgccgacgg cacaaagcct gcggtgtacg cgacgctgag
    10141 ccgagaaggt cagcagcagg cactgcaagg cgaacaaagt gggctgtttg cgcgcgttgc
    10201 ggctggggcg tggcttttgc gcgtcgatgc agccggtttt ctttcgcggg agcagacggt
    10261 gttcattagc gatggtggcg agcagcgggt cgagcttacg ctccagcgtc gcggcgccca
    10321 gcccaaggtg cagcttggcc aaagcgagct ggtgctgcgc gaaccgctcg tgtttgtgcc
    10381 ggcgagcagt gtgctttccc cggacagcga gcggctgctg gatgaagtga gcgatctgct
    10441 gatccaccat ccagaggtcg cgcttttgca catcgaacac caggcggact tgtcgatgag
    10501 cgatccgctg cttctggaac agcaggtgat cgcggtgcgg gattatctgg ttcagcacgg
    10561 aaccgcgccc gaacgtctgg tggcgcggct tgtcgatggt ccgcggcggg ccccggcgaa
    10621 aatcgttctc aagctctcgg cagaacgccg tccggcgcct tagtcacatg gctctagaac
    10681 aaccttttcc gacgctgcgc attgccaggg attccgatct tgctctcatc gtggcgacgt
    10741 acaatgcgac cattgcgtcg cggcgcgtaa ctgcggatct ggttccggtg actgtcgaca
    10801 gccgccggcc ctggtttgcg gcc
//
LOCUS       DAORGN010000174        10805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2576_length_10805_cov_5.647721, whole genome shotgun sequence.
ACCESSION   DAORGN010000174 DAORGN010000000
VERSION     DAORGN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_10805_cov_5.647721"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKL17_16505"
     CDS             <1..498
                     /locus_tag="PKL17_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46386.1"
                     /translation="RLLARRNEDSALLHSVEGSFRLNLFSDVHLLGSVSYTYGSVLLS
                     GPMGGTEPFWRVPPLWGMGALQFRRPRSMLALAEIGARAAASQRRLASQDKLDATICP
                     LGMAQCDGSPWFVATYLRTSLRLSRQIYLSALLENLTNDTYRIHGSGVPGPGLGAHLT
                     LEGNL"
     gene            complement(495..1868)
                     /locus_tag="PKL17_16510"
     CDS             complement(495..1868)
                     /locus_tag="PKL17_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNK46387.1"
                     /translation="MLPRILAARTFSQQFEHIKLVASRDLLYRFLYAMPKGGDLHQHF
                     NLSNFAESWLLLATDPKVTQNNIFYTRARPRDCPNSPSTAMPRFSTIQKSVYDALPPC
                     QKEEYVSLSALLPEERALFISGLKLDKDEEGRQEFFDAIGQRIGDLGRDPNLAGAALV
                     DNMRRFSAEGLRYMEVFVVGPRFIDIYGQPIAVERGVQILRDRLLSPDALATGMTVRF
                     LATIVRHHPDAESQIEKAYELVAKHRDLFVGINVAGREEDGRGHALRFVDVFRRMRTH
                     YSNIPLSLHAGESEAPGTQVKDTLLLGANRIGHGTNLLSDSSTVLLMRGNRFLIETSL
                     LSNQLLKYVPDIKVHPFPEFLRIGIPVCLNTDDRGAFDSNLTDEFFAAVTNFNLTWEE
                     IIAIGRNSIQHSFAEPPVRDRLLREYEAAVKRFELQYFGDDWADKLRLINLPKSGYAR
                     RKLGIGP"
     gene            complement(2068..2144)
                     /locus_tag="PKL17_16515"
     tRNA            complement(2068..2144)
                     /locus_tag="PKL17_16515"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2107..2109),aa:Glu,seq:ttc)
     gene            complement(2307..3122)
                     /locus_tag="PKL17_16520"
     CDS             complement(2307..3122)
                     /locus_tag="PKL17_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46388.1"
                     /translation="MVHAKIEPSAAETTRRARMPVLHRYTVPVVGLDPAHDGLRIAHL
                     SDLHVGSLTSHRFIRDAVEMAQAETPDLVLMTGDFVCYSPKYVPLLGGLVRGFAVPTV
                     CVLGNHDYWTDGHGVTSELLREQYTVLRNQHTRIQVRSAPLTIVGVDDAVTRQGDVQR
                     AFSGIHKTDSRIVLSHVPSLAVPASEFGGGLILSGHTHGGHVQIPGLTDRIFRKLGYD
                     FVRGFYRVRDSLLYVNSGVGSSSVPIRAGAPAELAILTLRTAKLGPNDHPVIP"
     gene            3176..3796
                     /locus_tag="PKL17_16525"
     CDS             3176..3796
                     /locus_tag="PKL17_16525"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNK46389.1"
                     /translation="MPLTSLRRSSFVALAVGYCALAFGCTPYIPRYDYEVEPDPTRLE
                     YLIGPGDIIDIRQWKNPDVSGRVRVLPDGNISVPLLGQVQAAGLSARQLRDKLAKGLE
                     KYIAQSADMQTLTVAVDEFQSYTVSIMGEVAQPGHYQPGHYVTVLDAIALAHGLTPYG
                     KGEKMFILRRSAAAQRKIPVSYPGLTAGKHPEMNIYLMRGDVLVVP"
     gene            3793..4572
                     /locus_tag="PKL17_16530"
     CDS             3793..4572
                     /locus_tag="PKL17_16530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNK46390.1"
                     /translation="MTQPKRDTVDVLQVRFPRLYAEEAVAFLLGKLRSGESAGVCFPD
                     MSTLNQAFEKPAFRRLLQRRMIVLPDGAGVMWVANLRGKPLPANLNGTDMVPRFFAAA
                     PQGTTVFLLGGQMGVAERTAERFAARFAHLRFVGHSHGYLSGSDEAELIDRLAQPRPQ
                     IVLIGMGNPLQVEFIDRHLDDPRLRGTLWFAVGGQFDYYGGTLRRAPSLLRRARLEWL
                     YIVAQQPHKLSRYFVGIPKYLSRCAFAALTNAHDIPEGELS"
     gene            4569..5378
                     /locus_tag="PKL17_16535"
     CDS             4569..5378
                     /locus_tag="PKL17_16535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK46391.1"
                     /translation="MSVKATVRGLVKRGLRQAVATAGMLSSVDESPRILCYHGVCDDP
                     PDEWSVTPAQLRQQLAIVCGERNPVSLPQLMSWFFDGTKLPPRAVAITFDDGYQDVLR
                     HAAPILAAHGILGAAFVISGLIDGKMRDDSYVPTRPLLSWDEVRELAAAGFAIGSHTL
                     THPILAQQSSDVVERELRESRQRLSDVLGQPIDLLAYPYGTRQTVSLRDRELAKAAGY
                     QAAFLDSIGPLSRGQDRYALARCKVLGTDSLQVFRASLCGQLDLWRNVEDR"
     gene            5382..6980
                     /locus_tag="PKL17_16540"
     CDS             5382..6980
                     /locus_tag="PKL17_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46392.1"
                     /translation="MRRAWLQVAGLVRSAADIRLPSLLYGVSVTAPLPSGELLAIDTS
                     VAETCPGVVCVLTADSALSGEAQRYAPLLRELFSSPIRHAGQIVALVVADSRSAALVA
                     ARKINLRVRSQSAHLTIAAALSADATGSLLRSQSGAAADAFAQSKTRFVQTYRLAARL
                     AGWSGDGVATAVWRDGGLTLFTVGTPTPELLGRLGLAPERLLVLASSVGAEQVASGPQ
                     VLLAAIAALQLVRPVQVSVSSLAAAGVVCEQVQTVQLGLDDQDRPCALLLQSAIGTRH
                     RLPSDALAAPTALGELYGFSAKELSVLPARVALPTTPPQMDGFAIDAAVDELAFLLST
                     DPLALRLRWLDGKDSLAAKTLRACLLAISQRMAATDSPNASKNVGVAAAIHCLPSGEV
                     VVGAHCMWAKREQGRPRLVGHVCALGLLGIVDESAARPIIMRQIEKARRLVLRTELAC
                     LPRSGYTDEHPVDDSDASEVAVCDVMLLPAGFDRAPSVTTQAISALAESGVAAAALSA
                     LSLAIGERHRSLPAAGHFLRVAAS"
     gene            7163..8251
                     /locus_tag="PKL17_16545"
     CDS             7163..8251
                     /locus_tag="PKL17_16545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46393.1"
                     /translation="MSASLRKTSVAALVCGVALSSSLALAAEPVVSADEPQTTSVTQS
                     TTTTVTTNAPVQVAPLPPPPTVPQTVIAPVQVAPQPMPQPMPQTVIAPVQVAPQPFAP
                     PPPPVGFAGGYQGPIYFNGPVWIIPAQPGGQAMVQPALPLQLPPPPVYVQPPRFSPPP
                     RVIVPQPLSRPRVTPLIAKQPPRGPVFGIGVRFTSLGVRSQEVFGDKPTLLGGGVQMR
                     FRNQGHWGFELALDALKTNIADGAYVRTSYPFTFSPMLYIFKNRPENHFNIYATAGFG
                     LMASDITLYQDSRQERNQQFWEVLGQAGGGLELRFTRLALFADVRAIGMLLDQGSEPG
                     KFYDGVSSGPIAPQSIGYKANLGAMLWF"
     gene            8341..9738
                     /locus_tag="PKL17_16550"
     CDS             8341..9738
                     /locus_tag="PKL17_16550"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNK46394.1"
                     /translation="MSNPDSPPPNAERKSDELFELEFEGRTVAARPGDTVASALIASG
                     VQVLSRSPKFHRPRTVFCLSGSCGSCLLRIDGKPNTRACQVKAEPGLRCARQNAWPSA
                     DYDLFSAADMMFPVGMDHHTMFTSPRPLNRLMQKVVHQLGGLGRLPDEDTAAEGALSL
                     VCRHVAVLVVGGGPAGLAAATAAAQRLSGSGQVLLIDAQPEAGGSYLCHPELGRESAR
                     AALQLAQSTGVEVLSATAAFGFYREDSHPNQPVGRQGLLGAASEKALYKLSADRYIYA
                     TGAAAQNLLFVDNDRPGVLSGRAVGRLLACHGIRVGERPLVVGQGEYADSLVAALRRS
                     GQTVMTIDGEHEKITRALGGSWVEGAEICDVRGVHRTVSCDLIVIATPPAAASELPVQ
                     HGLVVDLRQPGGFVVRSSGSGQTVQPDVFVCGEVTGPMSAEEAARHGACVGQTVAASL
                     IGAGATVTETPSVAG"
     gene            9754..10068
                     /locus_tag="PKL17_16555"
     CDS             9754..10068
                     /locus_tag="PKL17_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK46395.1"
                     /translation="MSEKCIVCPCEDVTLADVEQAISLGYLDIDEVKRFTGLGTGPCQ
                     GKQCMVSTCLLLQQAAERDPDRQKRQPESRVEPHPFTQRPPVMPVSLYVLAAGKGVTR
                     GS"
     gene            10076..>10805
                     /locus_tag="PKL17_16560"
     CDS             10076..>10805
                     /locus_tag="PKL17_16560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK46396.1"
                     /translation="MPAAAQTLAAEAETVIIGGGIMGLGIAYELARRGRSDVHVLEQE
                     YLTSGASGRNGGGVRMQWSTEMNIQLMQESVDICRGFASELHINVWLRQGGYLFLIRG
                     ESERAQLERNVALQNRLGVPTRILSPQDALKIVPELNPRSFVAACYNPRDGVVFPWPF
                     LWGYAHAAAELGVKIHTYTKVTAIEARGPHDFVVSTSKGTIRAERVINAAGAWSPAIA
                     RMVGVELPNHPHRHEILATESLKPF"
BASE COUNT         1932 a         3105 c         3573 g         2195 t
ORIGIN      
        1 agactgctcg cgcgtcgcaa cgaagactcg gcgctgcttc actccgtcga ggggagcttc
       61 cgtctgaacc tgttttccga cgtccatctc ctgggcagcg taagctacac ctacggatcg
      121 gtgctgctat cgggaccgat gggcgggacg gagccgtttt ggcgggtgcc accgctgtgg
      181 ggaatgggcg cgcttcagtt tcgacggcct cgatcgatgc tggcgctggc ggaaatcggt
      241 gcgcgagcgg cggcttcgca gcgacggctg gccagccaag acaagctcga cgcgacgatt
      301 tgtccgctcg gcatggcgca gtgcgacgga tcgccgtggt ttgtcgcgac gtatctgcgg
      361 acctcgctgc gactgtcgcg tcaaatctac ttgtcggcgc tcttggaaaa tctcacaaac
      421 gacacgtatc gcatccacgg atcgggcgtt ccggggcccg gacttggtgc gcacctgacg
      481 ctggaaggca atctttaggg gccaatcccg agcttgcgac gggcgtaacc cgacttgggc
      541 aggttgataa gccgcagctt gtcggcccag tcgtcgccaa agtattgaag ctcgaagcgc
      601 ttcaccgcgg cttcgtattc gcgcagcaga cggtcgcgca cgggcggctc ggcgaacgag
      661 tgctgaatcg agttgcgccc gatcgcgatg atttcttccc aagtcaggtt gaagttggtg
      721 acggcggcga aaaattcgtc cgtgaggttg gaatcgaagg cgccgcgatc gtcggtgttt
      781 aggcagaccg ggatgccgat gcgcaaaaat tcggggaatg gatgcacttt gatatcaggg
      841 acgtacttga gaagctgatt cgagagcagc gaagtttcga tcagaaaacg gttgccgcgc
      901 atcagcagca ccgtcgagga atccgacagc agattggtgc cgtggccaat gcggtttgcc
      961 ccaagcaaga gcgtgtcttt gacctgcgtg cccggcgctt cgctttcccc cgcgtgcagc
     1021 gacagtggaa tgttgctgta gtgggttcgc atgcggcgaa acacgtcgac aaaccgcagc
     1081 gcatgaccgc gcccgtcttc ttctcgaccc gcaacattga ttcccacaaa caggtcgcgg
     1141 tgcttggcca ccagctcata cgctttttca atctgcgatt cggcgtcggg atgatggcgc
     1201 acaatcgtcg ccaggaatcg caccgtcatc cccgtcgcca gtgcgtcggg ggaaagcagg
     1261 cggtcacgca aaatttggac gcctcgctcg acggcaattg gctggccata gatgtcaatg
     1321 aagcgcggcc cgacgacaaa tacttccatg tagcgcaggc cttcggcgga aaagcggcgc
     1381 atgttgtcga ccagcgcggc cccggcgagg ttcgggtctc tgcccaaatc accaatccgc
     1441 tggccaatcg cgtcgaaaaa ttcctggcga ccttcttcgt ctttgtcgag cttgagcccg
     1501 gaaataaaca gcgctcgttc ttccggcagg agcgcggaca atgagacgta ttcttccttt
     1561 tgacacggcg gcagtgcgtc gtagaccgac ttctggatgg tcgaaaagcg gggcattgcg
     1621 gtcgacggac tgttcggaca gtcgcgtggg cgcgctcgcg tgtagaagat gttgttttgg
     1681 gtgactttgg ggtccgtcgc gaggaggagc cacgactcgg cgaagttcga gaggttgaag
     1741 tgctggtgaa ggtctccgcc tttcggcatc gcgtagagga agcgatacag aagatcccgc
     1801 gaggcgacca gcttgatgtg ctcgaactgt tgcgaaaatg ttcgagctgc cagaatccgc
     1861 ggcagcaagc agaccactaa gagggtcagc caggccaatt gccgtcggaa acgggggaaa
     1921 agttcaagca aaaatctagt catggatcgc tgttggccca gccaaaatag catgtgccac
     1981 tacacgtccc atcaactaaa cacaaccaag aacaaaatag aatcaaagac ccgtcccctt
     2041 cttagggacg ggatactact gcacttttgg cgaccccaac ggggtttgaa cccgtgttac
     2101 caacgtgaaa ggctggtgtc ctaggccggc tggacgatgg ggtcagaaat gagaattcaa
     2161 agttgcgggc ttttgccaac agcgccgcaa gacgacccgc attttccatg gcggcaggtc
     2221 cttgtcaacc cactattctg ggaaagctgc tttttttatg aaaactcgcg gctttcccga
     2281 gagagccgtt tgtttcggct gcgggctcag ggaatgaccg gatggtcatt tgggccaagt
     2341 ttggcggtgc gcagggtcaa aatcgcaagc tctgccggcg cgccagcgcg gatcggcacc
     2401 gaactggagc caacgccgga gtttacgtac agcagcgagt cgcggactcg atagaaaccg
     2461 cgcacaaagt catagccgag cttgcggaag atgcgatcgg tcaggccggg aatctgcaca
     2521 tgaccgccgt gggtgtgacc ggacaggata aggccaccgc caaactcaga ggccggcaca
     2581 gccagcgacg ggacgtggct cagcacgatg cggctgtcgg tcttgtgaat gcccgaaaac
     2641 gcgcgctgca catcgccttg ccgcgtcact gcatcgtcga caccaacgat ggtcagcggt
     2701 gccgatcgca cctggattcg ggtgtgctgg ttgcgcagta cggtatattg ctcacgcagg
     2761 agttctgacg tgacgccgtg tccgtcggtc cagtagtcat ggttgccgag cacgcagacg
     2821 gttggcaccg caaaaccgcg gaccaggcca ccgagcagcg gcacgtattt ggggctgtag
     2881 caaacgaaat cgccggtcat gagcaccaga tcaggtgttt cggcctgggc catctcgacg
     2941 gcgtcacgaa tgaaccgatg ggaagtcagc gacccaacgt gcagatcgct caagtgggcg
     3001 atgcgcagcc cgtcgtgcgc tggatcgagc cccaccaccg gcacggtgta gcgatgcagc
     3061 actggcatgc gggcacggcg ggtggtctcg gcggcgctcg gctctatctt ggcgtggacc
     3121 atggccccaa gtatagaggg cagccgaaat cgttgtggta gcattcgccg tcaccatgcc
     3181 gcttacctct ttgcgtcggt cgtcgttcgt tgcgcttgcc gtcggatatt gcgcgctggc
     3241 tttcggctgt acgccgtaca ttccgcgcta tgactacgag gtcgagcctg acccgacgcg
     3301 gctggaatat ctgatcggtc ccggcgacat catcgacatt cggcagtgga aaaatcccga
     3361 tgtatcgggc cgggtgcggg tgctgcccga cggaaacatc tctgtgccgc tgttgggaca
     3421 ggtgcaggcg gctggtcttt cggcgcggca gcttcgcgac aaactggcca aagggcttga
     3481 aaaatacatt gcgcaaagtg ccgatatgca gacgctcacc gtcgctgtcg atgaatttca
     3541 aagctacacg gtgagcatca tgggtgaagt cgcgcagcca ggacactatc agccagggca
     3601 ctacgtgacg gtgctcgacg cgatagcgct agcgcacgga ctgacgcctt acggcaaggg
     3661 cgaaaagatg ttcattttgc gacggtcagc ggcggcgcag cgcaaaattc cggtgtcgta
     3721 tccgggtctt accgccggca aacatccgga aatgaatatc tatctgatgc ggggcgacgt
     3781 gttggttgtg ccatgacgca gcccaaacga gacactgtcg atgtgcttca ggtccgtttc
     3841 cctcgactgt atgccgagga agcggtggca tttttgctgg gcaagctgcg cagtggcgaa
     3901 agcgcggggg tctgtttccc cgatatgtcg acgctgaatc aggcgttcga aaaaccagcg
     3961 tttcgacggc tgctgcagcg ccggatgatc gtgctccccg acggtgcggg tgtgatgtgg
     4021 gtggcgaact tgcggggcaa gccgctgccg gcaaacctaa acggaacgga catggtgccg
     4081 cggttttttg ccgctgcgcc acaggggacg acggtgtttt tgctcggggg gcagatgggg
     4141 gtggccgagc gcactgccga gcgttttgcc gcgcgctttg cccatctgcg ctttgtcggt
     4201 cattcgcacg gctatctgtc gggaagcgac gaggcagagc ttatcgatcg gctggcgcag
     4261 ccgcgtccgc agatcgtgct tatcgggatg ggcaatccgc tgcaagtcga gttcatcgac
     4321 cgtcatttgg atgaccccag gctgcgcgga acgctgtggt ttgccgtcgg tggtcagttc
     4381 gattattacg gcggaacgct gcggcgggcg ccgtctttgc tgcggcgggc gcgactcgaa
     4441 tggctgtata tcgtcgcgca gcagccgcac aagctgagcc ggtatttcgt cggcattccg
     4501 aaatatttgt cgcgctgtgc cttcgcggcg ctgacgaacg cccacgacat ccccgaagga
     4561 gagctttcgt gagcgtcaaa gcgacggtgc gcggtcttgt caagcgtgga ctgcggcaag
     4621 cggtggcgac ggcaggaatg ttatcgtccg tcgacgaatc accgcgcata ctttgttacc
     4681 acggggtctg cgacgatcca cccgacgagt ggtcggtgac gcccgcgcag cttcgtcagc
     4741 agcttgcgat cgtctgtggt gaacgcaacc cggtgtcgct gccgcagctc atgtcgtggt
     4801 ttttcgacgg gacaaagctg ccgcctcgcg cggtggcgat cacatttgac gacggctatc
     4861 aggatgtgct ccggcacgcg gccccgatct tggcggcgca cgggattttg ggggcagcgt
     4921 ttgtgatttc cggtcttatc gacgggaaaa tgcgcgatga cagctatgtt ccgacgcgac
     4981 cgttgctttc ctgggatgaa gtgcgagagc ttgccgccgc gggctttgcg ataggatcgc
     5041 atacgctgac gcacccgatt ttggcgcagc agtcgtccga cgtggtagag cgggagctgc
     5101 gcgaatcgcg gcagcggctg tccgacgtat tgggccagcc gatcgacctg ctggcttacc
     5161 cgtatgggac gcgacagacg gtgtcgctgc gtgatcgcga gctggcgaaa gcagcgggct
     5221 accaagcggc gtttctcgat tcgatcggtc ctttgtcgag aggacaagat cgctatgcac
     5281 tggcgcgttg caaagtgctg gggacggaca gcttgcaggt gtttcgcgcg tcgctgtgcg
     5341 gacagctcga cctgtggcgc aacgtggagg atcgctaacc gatgcggcgc gcctggctgc
     5401 aagtggcggg tcttgtgcgg agtgccgccg acatccgttt gccgtcgctg ctctatggtg
     5461 tttcggtcac tgcgccgctt ccgtcgggag aactgctggc gatcgatacg tccgtcgcgg
     5521 aaacgtgtcc aggggtggtt tgcgtcctga cggcagactc ggcgctttcg ggggaagctc
     5581 aacggtatgc tccgctgctg cgagagctgt tttcttcgcc gatacgtcac gctggccaga
     5641 tcgtggcgct ggtggtggcg gattctcgat cggcggcgct cgtcgctgcc cggaaaatca
     5701 atctgcgcgt gcggagccag tcggctcatc tgacgattgc ggcggcgctt tcggcggacg
     5761 cgacagggtc tcttttgcgc agtcagtccg gggccgccgc ggatgcattt gcgcagtcga
     5821 agacgcgctt tgtgcagaca tatcgactgg cggcgcgcct ggcgggctgg tcgggtgacg
     5881 gtgtcgcgac cgcagtgtgg cgagacggcg ggctgacgct gtttaccgtt ggaacaccga
     5941 ccccagaact gcttggacgg ctggggcttg cgcccgaacg gcttctggtt ttggcttcat
     6001 ccgtcggtgc ggaacaggta gcaagcggtc cgcaggtgct gctggcggcg atcgcggcgc
     6061 tacagcttgt gcgtccggtg caggtttctg tgtcgtcgct cgctgccgct ggggtggtct
     6121 gcgaacaagt gcagacggtg cagcttgggc tcgatgacca ggatcgtcct tgcgcactgc
     6181 ttctgcaatc ggcgatcggg acgcgacacc ggctgccaag tgatgcgctc gctgctccga
     6241 cggcactggg cgagctctat ggtttttcgg caaaagagct gagtgttctg ccggctcggg
     6301 ttgcgctacc gacgacacca ccgcagatgg atggttttgc tatcgacgct gcggtggatg
     6361 aacttgcgtt tctgctgtcg accgatccgc tcgcacttcg gctgcgctgg ctcgacggaa
     6421 aagacagctt ggctgccaag acgttgcggg cgtgtctttt ggcaatttcg cagcgaatgg
     6481 cggcgacgga ttcgcccaac gccagcaaaa acgtgggcgt cgccgccgcg attcattgct
     6541 tgccatcggg cgaagttgtc gtcggggccc actgcatgtg ggccaagcga gagcagggtc
     6601 gtccgcggct tgttggtcat gtctgcgcgc tgggtctttt aggcatcgtc gacgagtcag
     6661 cggccaggcc gatcatcatg cggcagatcg aaaaagcccg acggctggtg ctgcggacag
     6721 agcttgcctg tctgccgcgg tctggctaca cagacgaaca ccccgtcgac gattcagatg
     6781 cgagcgaagt ggcggtttgt gacgtaatgc tcttgccggc tggctttgac cgcgctccgt
     6841 ccgtgacgac gcaagccatt tcagcgctgg ccgaaagtgg tgtggcggcg gcggctctca
     6901 gcgcgctgtc gctggcgatc ggcgaacgtc atcggagctt gccggcagcc gggcacttct
     6961 tacgtgtagc tgcgtcgtga cttgggtggt ttttttcggg tgtaggtggg tgcgttggaa
     7021 aaactgcaaa aactccgtcg aggagcttgt gtcattgaaa ggcctgccaa gctgtggcat
     7081 ccgtattgaa tagacagtcc tgcaattgca aggaggcctc atgcggtcct tgttcgtaag
     7141 cactcagcgt cgccgtttca gaatgtccgc ttcgttgcga aagacctctg tcgctgcgct
     7201 tgtttgcggc gtggctctat cgagctcgct cgcgctggcc gctgagccgg tggtatccgc
     7261 cgatgagcct cagacgacct cggtaaccca gtcgacgacg accacggtta cgaccaatgc
     7321 gccggttcag gttgcaccgc tgccgccgcc accgacggtt ccgcagaccg tgatcgcgcc
     7381 ggttcaggtt gcgccgcagc cgatgccgca gccgatgcca cagacggtga tcgcgcctgt
     7441 ccaggttgca ccgcagccgt ttgcaccgcc cccgccgccg gttggctttg cgggtggata
     7501 ccagggtccg atctatttca atggccccgt gtggattatt ccggcgcagc ccggcggaca
     7561 agcgatggtc cagccggcac tgccgctgca gcttcctccg ccgccggtgt acgtgcagcc
     7621 gccgcgcttt tcgccgccgc cgcgggtgat tgtgccgcag ccgctgtccc gtccgcgcgt
     7681 gacgccgctt atcgccaaac agccgccgcg tggcccggtg tttggcatcg gcgttcgttt
     7741 tacctcgctc ggtgttcgct cgcaggaagt gttcggtgac aagccgacgc tgcttggcgg
     7801 tggcgtgcag atgcgcttcc gcaaccaggg tcactggggt ttcgagctgg cgcttgacgc
     7861 actgaagacg aacatcgcag acggtgctta cgtgcggacg tcgtatccgt tcacgttttc
     7921 gccgatgctg tacatcttca agaaccggcc cgagaaccac ttcaacatct atgcgacggc
     7981 gggcttcggt ctgatggcga gcgacatcac gctctatcag gactcgcgtc aggaacggaa
     8041 ccagcagttc tgggaagtgc tgggacaagc gggtggcggt ctggagcttc gctttacccg
     8101 tctcgcgctg ttcgccgacg tgcgtgcgat cggcatgctg ctcgaccagg gcagcgagcc
     8161 cggcaagttc tacgacggtg tcagcagcgg cccgatcgct ccgcagagca ttggctacaa
     8221 ggccaacctc ggcgcaatgc tctggttcta gaaccttcgc cacttcgtcg ccgcatcgag
     8281 agggacttcg caatcaccca ctcgctgcgg acctgacgta gcttctggca gccttttctc
     8341 atgagcaatc ctgactcccc tccgccgaat gccgagcgca aaagcgatga gctgtttgag
     8401 ctagagttcg agggtcgtac cgtcgctgcg cgtccgggtg atacggtggc ttccgcgctg
     8461 atcgcttccg gtgtgcaggt gctgagccgt tcgcccaagt tccacagacc gcgcacggtg
     8521 ttttgcctga gcggttcgtg cggctcgtgt cttctgcgga tcgacggcaa gcccaacacc
     8581 cgcgcctgtc aagtcaaggc cgaaccaggt ctgcgctgcg cacgtcagaa tgcgtggccg
     8641 tcggcagact acgatctgtt ttcggctgcc gacatgatgt ttcctgtcgg catggaccac
     8701 cacacgatgt ttacatcgcc gcgtccgctc aatcggctga tgcagaaagt ggtgcatcag
     8761 cttggtggac ttggacgact gcctgacgaa gacacggcgg cggaaggggc gctgtcactg
     8821 gtctgtcgac acgtcgcagt gctggtggtt ggcggcgggc cagcgggact tgcggcagcg
     8881 acggcagcgg cacagcgcct ttcggggtct ggacaggtgc tgctgatcga cgcccagccc
     8941 gaagcaggcg gcagctatct gtgtcatcca gagctgggtc gtgagagcgc gcgggcggcg
     9001 ctgcaactgg cacaatcgac cggcgtcgag gtattgtcag cgacggcagc gttcggtttt
     9061 tatcgggaag acagccaccc caatcagcct gtcgggcggc agggactgct cggggcggcg
     9121 tcggaaaaag cgctctacaa actgtcggcg gatcgctaca tttacgcgac gggagcagca
     9181 gcgcaaaacc tgctgtttgt cgacaacgat cgcccaggcg ttctgtcggg tcgtgcggtc
     9241 ggtcggctgc tggcgtgcca cggaatccgc gtcggtgaac gtccgctggt cgtcgggcag
     9301 ggagaatacg ccgattcgct ggttgcggcg ctgcgacgca gtggtcagac ggtgatgacg
     9361 attgacggcg agcacgagaa aatcacccgt gcgctcggtg gcagctgggt ggaaggggcg
     9421 gaaatttgcg acgtgcgtgg cgttcatcgg acggtgagct gtgatctgat cgtgattgcg
     9481 actccaccag cggcagcatc ggaattgccg gttcagcatg gcctggtcgt cgacctgcgc
     9541 cagcccggag gttttgtggt gcgctcgtcc gggagcggcc agacggtgca gcccgacgtc
     9601 tttgtctgcg gtgaagtgac cggcccgatg tctgccgaag aagccgcccg gcacggcgcc
     9661 tgcgtgggcc agacggtcgc ggcgtcgctt atcggggcgg gggcgactgt gaccgaaacg
     9721 ccgtctgtcg ctggctagtt ctgcgaggct gtcatgtcgg aaaagtgcat tgtgtgccca
     9781 tgtgaagatg tgacgctcgc cgatgtcgag caagcgataa gccttggcta tctcgatatc
     9841 gacgaagtca agcgcttcac tgggctgggg acggggccgt gtcagggcaa acagtgcatg
     9901 gtcagcacct gcctgctgct tcagcaggcg gcggagcgtg atcccgatcg gcagaagcgc
     9961 cagcccgaat cgcgtgtaga accgcatcca tttacgcagc gtccgccggt gatgccggtt
    10021 tctctgtacg ttttggctgc gggcaaaggg gtgacccgtg gaagctagcg gacgcatgcc
    10081 tgccgcagcg cagaccctcg ctgccgaagc cgagaccgtg atcatcggcg gcggcatcat
    10141 gggcctgggc atcgcctatg agctggcacg gcgcggtcgc agcgacgtac acgtacttga
    10201 gcaagagtac ctgacgagcg gcgcctcggg tcgcaatggc ggcggcgtgc gcatgcagtg
    10261 gtcgacggaa atgaacatcc agctcatgca ggagtcggta gacatctgtc gcggcttcgc
    10321 aagtgagctt cacatcaacg tctggctgcg tcagggcggt tacctgtttt tgattcgcgg
    10381 cgaaagcgaa cgggcgcagc ttgagcggaa cgtcgcgctg caaaatcgac tcggggtgcc
    10441 gacgcgcatt ttgtcgccgc aggatgccct gaagatcgtc cccgagctca atccgcgctc
    10501 gtttgttgcg gcttgttata acccccgcga cggtgtggtc tttccgtggc cgtttttgtg
    10561 gggttatgcc cacgcagcgg cggaactggg cgtcaaaatt cacacctaca ccaaagtcac
    10621 ggcgatcgaa gcgcgtgggc cgcacgactt tgtggtgtcg acgagcaaag gtacgattcg
    10681 cgccgagcgg gtgataaatg ccgccggagc gtggtcgcca gcgattgccc gaatggtcgg
    10741 cgtcgagctg ccgaatcatc cgcaccgtca cgaaatcctc gcgaccgaat cgctgaagcc
    10801 gtttc
//
LOCUS       DAORGN010000175        10731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2606_length_10731_cov_5.568471, whole genome shotgun sequence.
ACCESSION   DAORGN010000175 DAORGN010000000
VERSION     DAORGN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_10731_cov_5.568471"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKL17_16565"
     CDS             complement(<1..227)
                     /locus_tag="PKL17_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46397.1"
                     /translation="MAETGGEKDEKPWSPPTSFEEYRLLWPLGRGGMGEVYLGHDTVL
                     DRPVAIKFVMNLEADEQVRERYLIEARAAAR"
     gene            361..1731
                     /locus_tag="PKL17_16570"
     CDS             361..1731
                     /locus_tag="PKL17_16570"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNK46398.1"
                     /translation="MKRMVRPVLGVLLGLTLAATGCTEALYPPRPQVLPGPALADPPN
                     SRVNMHISLTESGLRDVIEATVPQQGEVPFSFLGQRKLAWKRTPIELRFNNAASTIGV
                     KATITAQASVATNPTFTITLTADVQPALGSDYVAQLQNPQVQVQSDDKLLKAAEWSAG
                     ALSLIRDEVEKRLRELRINLRPLLADNYEKLAKPVSFPVGDASACVKLGVQSIEAGPT
                     VLSGGFEKDLSLVVAPSVTLPCSPEKGVAAGSGSLPPLHNVASIPSGPFEVTIPIAAT
                     YPELQKAMKQAFTDGKLHFDPDYPELYLEKPELYASGGRIVTKLHLNGYVKKGFKISL
                     EGDLFMSGSPEIVDNQLSVPNLEPTIETSSALLKLKTSLDAEKMKKEIRQALRLDIGS
                     RLQAVRDKVSKDLVVKHKLGQNVEACVKADIGRVEVSGLFAHDAYLRMYVKVAARAAA
                     YLPCPH"
     gene            1792..1874
                     /locus_tag="PKL17_16575"
     tRNA            1792..1874
                     /locus_tag="PKL17_16575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1825..1827,aa:Leu,seq:caa)
     gene            complement(1928..3451)
                     /locus_tag="PKL17_16580"
     CDS             complement(1928..3451)
                     /locus_tag="PKL17_16580"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HNK46399.1"
                     /translation="MAKPSKRPVSGKPPVATEEANIDSPRKLMHGAVPDVTHAIGGTP
                     IVRLSKVATHVRSSIYVKCEYLNPGGSMKDRIALNIIRDAEKKGLLKPGGVIIEATSG
                     NTGAGLAMVAALRGYQCIFVMPDKMSAEKVANLRSLGAKVVLCPTAVDAEDPRSYYSV
                     TKRLVAETPNSFHANQYHNPANPEAHYLSTGPELWAQTHGELEVFVAGMGTGGTISGC
                     GKYFKEQNPKVKIVGVDPIGSIYYELKKTGRITKPFSYSVEGIGEDFVPTTMNLDIVD
                     EIIRVDDKDCFLLTRALVQKEGILCGGSAGAAVMGAIRYAEHLDEQGLPPQNIVVLLP
                     DSSQKYLSKIFDDNWMRDNGFLDTEDPLGTVSDIFASRPPRPAITAERTATLRQVIAL
                     LKEHGISQVPVIDRGRICGIVAEVDLLNHILVKGASLDSPIEPIIEADYQTVTPQTKV
                     RLLKTIFNDAKMVCVLASRPGTSSEGLSPSKAAVPSDSLLGVITKIDLIDYLATRRS"
     gene            complement(3454..4053)
                     /locus_tag="PKL17_16585"
     CDS             complement(3454..4053)
                     /locus_tag="PKL17_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNK46400.1"
                     /translation="MPTTHHPADASGSAVEQSCAVIEKILGLSASHRESDRGIFIDRG
                     LYVIKQGSSYVMISVAHTGENRDQALVRLTAQVVAGVRPEAALLRRLLILNGRLRFGA
                     FAYEPDGDLILFTHSLLGGATLNPNEVMAAVHDVALAADRYDDQIVAQYGGRRMQDVI
                     EESAWARLLKKAGDEPPHPHLDEEAAKKTPSSGGKKKGS"
     gene            complement(4222..4869)
                     /locus_tag="PKL17_16590"
     CDS             complement(4222..4869)
                     /locus_tag="PKL17_16590"
                     /inference="COORDINATES: protein motif:HMM:NF018866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HNK46401.1"
                     /translation="MSDFPVTDPTEPLAVGAADDPTVQAALRRSDRYLQEFIEQLSIC
                     PYAKQCREQGRLYRGVFLQVQPSPQAVANFLLSLDPERLYEIGLLLFPNFAGEGKVGE
                     SHPFERFVSDVRNQYGKLRKPPAFFIVAFHPQMPSNLSNPDVAVRFMRRSPDPTIQLV
                     RPEVIDAVRNPHHNRDELSTAIAEMGLRTVRSHGVEKITDALANIHQHERPVDKL"
     gene            complement(4879..7629)
                     /locus_tag="PKL17_16595"
     CDS             complement(4879..7629)
                     /locus_tag="PKL17_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46402.1"
                     /translation="MSTKTSPPPHSDGASAPLPSPDRLTTPAGRPPEPKRSRPPAEPR
                     SPITASASIIAESAPQAPERYGKYHLLERIGIGGMAEVWRAKTLGSEGFTKDLVIKRI
                     LPKFLGDEESIRMFIDEARLVAKLHHPNIVQIFDFDKEGDRYYLAMELVEGRDLRQSE
                     KAAAKAGIWFPLSVSIYIISEALKGLQYAHTRLDHGKPLGIVHRDISPHNILISFSGD
                     VKISDFGIAKVAERQPAPAGGTVKGKLSYMSPEQITGQALDARSDIYSMGIVFWELIT
                     RHRLFFGADEQDVVRRVRDGIVPPPRQYNPDIPEEVEKIVLGMLVANRKERTQTAAEV
                     VRQLMALPQYAYEAQPLGEFMGTLFPEQSRNWTKLLQDMLKKEPKRPIDKGMLAMTPL
                     PSTIPNPFPTAAPASAASGTEPAAKAPDSLSQSGKRPKRSLPPPPEAQSHETAQLRPA
                     EIAALATPHRPIPPPTSAAEPGGLSTLSTPAPQEPASRSNMSGSDQKTQIATEMPPEV
                     AAAIARGAPPPAAGARTVIAQAPSLPPQGAPPSAAPPGASPEGARTMIAQAPQIPSPP
                     PGAPVVATGPQQATMYATPAPQIPNPQSMAATLPPGARNASIPLTMRPAAVAFASSAP
                     NAGIPPMAKWIIGPAIAVIVALLTTALANRIWPPEPGAGPAGPSILAASRKIKIITDP
                     AGASVESNGKLLETKTPTEVKGDIGTTAKLRITLDGFDPIEMKVLFARDERPPLNIRM
                     PKLGSTKPKSGDPSQYDTQVGVQENPDSDKGTKPDDKTAKATDKNGKPDDKATKAEPE
                     DEDKKGKKGKKDKVAKTDGEEEEDGKGKKGKKDKVAKTDKGEKGDVKKEKPTLSVSVR
                     PYAVVYIDNKKIQQTPLRDYAVSPGSHTILLVNDIKGKRETIKIKVSAGEKVPPITRN
                     WD"
     gene            7942..9240
                     /locus_tag="PKL17_16600"
     CDS             7942..9240
                     /locus_tag="PKL17_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46403.1"
                     /translation="MHHSANATRLRHRCTVLLLTASLGLGGLASTGCQTLSTAQSGMQ
                     TGVSTAQTTKSTADQTKQLGTDLKGEVDKATGKKTDGAGGAAGDKPEDEDGKRLQAKS
                     SKLNQPVNDELNQTKNDLTDWKAYDLSGISSGKWVEFVLNWDEPDAEINLDIYDQVGA
                     QVVQSPGRAGVPQKNVPLRVLTPGIYYARLTLQGNYPKKESVYTVVLKTGKNAALAKP
                     TSISGKKEGDAAAAGAAGGAPGAPGAPGAPGAPGGAPGMGGAPGMGGAPGMGGAPGMG
                     GYPPMGAMPGGMGGYPQIGMQPGMGMQPGMGMGMQPGMQGGAGGAGGAGGAGGAGGGA
                     GGGGGGDSSAIPEGAIVGRIVQSTVEEGKRILYLDKGSKAKLRVGMQGFILQGKEGGQ
                     KLDGGTFTITKVLGDSQAVGSTDYGKSLGSNNRFMIQKPK"
     gene            9334..10140
                     /locus_tag="PKL17_16605"
     CDS             9334..10140
                     /locus_tag="PKL17_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46404.1"
                     /translation="MFASSLLIMAAAGCKKDDVNAKIKNGPDPDTNSGVDAKRGGAKP
                     MQINRPETDSVSWPKLQRTKWKSIEIATPGKDNVLDCELRIDNTGADLNIDLFNSLGT
                     QIGFTPGPAPNKLKKLAVKLEELGTYFVRMQAAMPKDESDFSVICRWEEEAVVPDAPD
                     KPKKAVKRTPTEKPAKAETFEEKIEKGTEGRIKTSFREGGQMVMIVDKGAAAGVHEGT
                     EGTVLEGKSGRQPLDGGTFKILQVIDDSTSKAQSSVRSIGKNSRVVLFLK"
     gene            10172..>10731
                     /locus_tag="PKL17_16610"
     CDS             10172..>10731
                     /locus_tag="PKL17_16610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46405.1"
                     /translation="MSTPGQQDPLIGKVLADRFEILSRIGEGGTGVVYKAKQITVDRT
                     VAIKVLGAHVSSDPQWVKRFHNEARAAARLDHPNTVRMIDFGETKEGLLFIAMEYLHG
                     RPLSDEISRLGKIPPQRALRIISQVCQSLQEAHGQGIIHRDIKPDNVFLVEMKGSGDF
                     VKVLDFSVAKMDTPDAQLTRAGTVFGT"
BASE COUNT         2048 a         3037 c         3425 g         2221 t
ORIGIN      
        1 aaccgggcgg cggcgcgcgc ttcgatcaga tagcgctcgc gcacttgctc atccgcctcc
       61 aggttcatga caaacttgat ggcgacgggg cgatcgagga cggtgtcgtg gcccagatag
      121 acttcaccca tgccaccgcg accgagcggc cacagcagcc ggtattcctc aaacgaggtc
      181 ggcggcgacc acggcttttc gtccttctct ccgcctgttt cggccaagca tcccccagga
      241 gcccgcggta aggcaggtcc aactgctaag aataacaagg tgtaccaatt tgctcgcctg
      301 aaaatggcgg tacgcactgg gggggttggc ggttggcaga gcggtgctac catcctccgc
      361 atgaagcgaa tggtgcgccc tgttttggga gttctcctcg gcctgacgtt ggcggcaacc
      421 ggctgtaccg aggctcttta tccgccgcgt ccacaggtgt tgccgggtcc agccttggcc
      481 gatccgccaa actctcgcgt caacatgcac atcagcctga cggaatcggg cctgcgcgat
      541 gtcatcgaag cgacggtgcc gcagcaaggc gaggtgccgt tttcgtttct gggtcagcgc
      601 aagcttgcgt ggaagcgcac tcccatcgag ctgcgcttta acaacgccgc gtcgacgatt
      661 ggcgtaaaag cgacgatcac ggcgcaagcg tcggtggcga ccaatccgac ctttacgatt
      721 accctgactg ccgacgtcca gccggcgctg ggttccgatt atgtggcgca gctgcaaaat
      781 ccccaggtcc aggtccaaag tgacgacaag ctgctcaaag cggcggagtg gagcgcgggc
      841 gcactgtctt tgatccgcga cgaagtcgag aagcgtctgc gcgagctgcg catcaatttg
      901 cgaccgcttt tggcggacaa ctacgaaaaa ctggccaagc cggtgtcatt tcccgtcggt
      961 gatgccagtg cctgcgtcaa gcttggcgtg cagtccatcg aagcgggtcc gacggtgctt
     1021 tcgggcggat ttgaaaaaga cctgagcttg gtggttgcgc cgtctgtgac gctgccgtgc
     1081 agccctgaaa aaggcgtcgc tgccggttcc ggatcgctgc cgccattgca caacgtcgcc
     1141 agcattccgt cggggccgtt cgaggtcacg attccgatcg ccgcaacata tcccgaactg
     1201 caaaaagcga tgaagcaggc gtttaccgac ggcaagcttc actttgatcc cgactatccg
     1261 gaactttatc tggaaaagcc ggagctgtat gcgtcgggcg ggcgcatcgt cacgaagctg
     1321 cacttaaatg gctatgtcaa aaagggcttc aagatttcgc tcgaaggcga cttgtttatg
     1381 tcgggctcgc cggaaatcgt cgacaaccag ctctcggtgc ccaacctgga accgaccatc
     1441 gaaaccagca gcgcgcttct gaagctgaag acctcgctcg acgcggaaaa gatgaaaaaa
     1501 gaaatccgcc aagcgctgcg gctcgacatc ggcagtcggc tgcaagcggt gcgcgacaaa
     1561 gtcagcaaag acttggtcgt gaaacacaag ctggggcaaa acgtcgaagc ctgcgtcaaa
     1621 gccgacatcg gtcgcgtcga ggtgtccgga cttttcgccc acgatgccta cctgcggatg
     1681 tacgtgaaag tggcggcacg ggcggcggcg tacctgcctt gtccgcactg atgaaaaaat
     1741 cgtagggagt tgcggcaact tgtggtagct tgccgctcat tgttggtccg cgccgaagtg
     1801 gcggaatggc agacgcaggg gactcaaaat ccctcgtcct ccgggacatg agggttcgac
     1861 tccctccttc ggcaccgaaa gcgacgtagt cagatggctg cgtcgctttt tttgttttgg
     1921 gggaacgcta gctgcggcgg gtggccaggt agtcgatgag atcgatcttg gtgatgacgc
     1981 cgagcaagct gtccgacgga acggctgctt tgctcggcga cagtccttcg ctgcttgtgc
     2041 cagggcgcga cgcgagcacg cagaccattt tggcgtcgtt gaaaatcgtc ttgagcagcc
     2101 gcactttggt ctgcggcgtc accgtctgat aatccgcttc aatgatcggc tcgatcggcg
     2161 aatcgagcga cgcgcctttg accaaaatgt ggtttagcag atcgacttcc gcgacgatgc
     2221 cgcagatccg accgcgatcg atcaccggca cctgcgaaat cccgtgttct ttgagcagcg
     2281 cgatgacttg gcgcagcgtc gccgttcgct cggcggtgat tgctgggcgt ggcgggcggc
     2341 tggcgaaaat gtccgacacc gtgccgagcg gatcttcggt atcgaggaag ccgttgtcgc
     2401 gcatccagtt gtcgtcgaag attttcgaca aatatttctg cgagctgtcg ggcaaaagca
     2461 ccacgatgtt ttgtggcggc agtccttgct cgtcgagatg ttcggcgtag cgaatcgctc
     2521 ccatgaccgc ggctccggcg ctgccaccgc acaagatgcc ttctttttgc accagcgcgc
     2581 gggtcagcag gaagcagtct ttgtcgtcga cgcgaatgat ctcgtcaacg atgtctaggt
     2641 tcatcgtcgt cggcacgaaa tcttcgccga tgccctcgac ggaataggaa aacggcttgg
     2701 tgatccgccc ggtctttttc agttcgtaat agatcgaacc gatcggatcg acaccgacga
     2761 tcttgacctt gggattttgt tctttgaagt acttgccgca gcctgaaatc gttccgccgg
     2821 tgcccatgcc agcgacgaaa acttccagct cgccatgggt ctgcgcccac agctcggggc
     2881 ccgtcgacag gtagtgggct tcggggttgg ctgggttgtg gtactggttg gcgtgaaacg
     2941 aattgggggt ttcagcgacg agccgttttg tcaccgagta gtagctgcgc ggatcttcgg
     3001 cgtcgaccgc tgttggacac agtacaactt tggcgccaag cgagcggagg ttcgcgacct
     3061 tttccgccga catcttgtcg ggcataacga aaatgcactg atagccgcgc agtgctgcga
     3121 ccatcgccag acccgccccg gtgttgcccg atgtcgcctc gatgatcacg ccgccgggtt
     3181 ttagcagacc cttcttttcg gcatcgcgga tgatgttgag cgcgatgcga tctttcatcg
     3241 agccgccggg gtttaggtac tcgcacttga cgtagatgct gctgcggacg tgggtcgcga
     3301 ctttgctaag ccggacaatc ggagtgccgc cgattgcgtg ggtcacgtcg gggactgcgc
     3361 catgcatcag cttgcgtgga gagtcgatgt ttgcttcttc cgttgcgacg ggcggttttc
     3421 cgctgacggg tcgcttgctt ggtttggcca tgactagctt cccttcttct tcccgcccga
     3481 cgacggcgtt tttttggcgg cttcttcgtc caggtgcgga tgcggtggct cgtcgccggc
     3541 tttcttcaaa agacgcgccc acgccgattc ctcgatgaca tcctgcattc gacgaccacc
     3601 gtactgcgcg acgatctgat cgtcgtaacg atctgccgcc agcgcgacgt cgtgaactgc
     3661 agccatgact tcgttcggat tgagcgtcgc gccaccgagg agactgtggg taaacaggat
     3721 cagatccccg tcgggttcat aggcgaaagc cccgaagcgc agccgaccat tcaaaatcag
     3781 cagccgacgg agcagcgccg cttccggtcg cacgccagcc accacttggg cggtcaaccg
     3841 aaccagggct tggtcgcgat tttccccggt gtgggcgacg gaaatcatca cgtaagacga
     3901 gccctgcttg atgacgtaga gtccccggtc gatgaagatg ccgcggtcgg actcgcgatg
     3961 cgaggcggag agcccgagga tcttttcgat cactgcgcag ctttgctcga cggcggaacc
     4021 tgatgcgtcg gcgggatggt gtgtggtcgg cataaacgct ccttcagcgg tctttgcacg
     4081 aggcagacgt cgagaacaaa gcggaagcct gtgtgcccgc gtttctcgac gacggtttct
     4141 agcagcaata tcacgccact agggtcactg acaatggcgc tgcctcggaa agcggaggca
     4201 aaatggctgg accgcgctgc gtcaaagctt gtcgaccggg cgctcgtgct ggtggatgtt
     4261 cgcgagcgca tcggtgattt tttcgacgcc atgactgcgc accgtccgga gccccatttc
     4321 ggcaatcgcc gtcgacagtt catcgcggtt gtggtgcgga ttgcgcactg cgtcaatcac
     4381 ttccgggcgc accagttgga tggtcggatc gggactccgt cgcataaagc ggaccgccac
     4441 gtcaggattg ctcaagttgc tgggcatctg cgggtgaaag gcgacgatga aaaacgccgg
     4501 tggtttgcgc agcttgccgt actgatttcg cacgtcgctg acgaagcgtt caaacggatg
     4561 cgattcaccg actttcccct cgccagcgaa gtttgggaac agcagaagtc caatttcgta
     4621 cagccgttcc ggatcgagcg aaagaaggaa gttcgcgacc gcctgtggcg acggctggac
     4681 ttgcaaaaac acgccccgat acagccggcc ttgttccctg cactgtttcg catacggaca
     4741 gatcgacagc tgctcgataa attcttgcaa atagcggtct gatcgacgga gcgcggcctg
     4801 cacggtcgga tcgtccgcgg caccgaccgc tagcggttcc gtgggatcgg tgacgggaaa
     4861 atcgctcatg gcagaaggct agtcccagtt gcgggtgatg ggcggtactt tttcgcctgc
     4921 cgagaccttg atcttgatcg tctcgcgctt gcccttgatg tcgtttacca aaaggatggt
     4981 gtgcgaaccg gggctcaccg catagtcgcg cagcggcgtc tgctggattt tcttgttgtc
     5041 gatgtagacg acggcatacg gacgcaccga gacgctcagc gtcggttttt ctttcttcac
     5101 gtcgcctttt tcgcccttgt cggtcttggc gactttgtct tttttgccct ttttgccttt
     5161 gccgtcttcc tcttcttccc cgtcggtctt ggcgactttg tcttttttgc ccttcttgcc
     5221 ctttttgtct tcgtcttcgg gctcagcttt ggtcgctttg tcgtcgggct tgccgttttt
     5281 gtccgtcgct ttcgcggtct tgtcgtcggg ctttgtgccc ttgtccgaat cgggattttc
     5341 ctgcacgccg acttgggtgt cgtattggct ggggtcgccc gacttgggct tggtgctccc
     5401 gagcttcggc atgcggatgt tgagcggtgg tcgctcgtcg cgggcgaaca gcactttcat
     5461 ctcgatcggg tcaaatccgt cgagcgtgat gcgcagcttg gcggtggttc cgatgtcgcc
     5521 tttgacttcc gtcggggtct tggtttcgag cagcttgccg ttggattcga cggatgcgcc
     5581 ggcaggatcg gtgatgatct tgatcttgcg cgatgcggcc aaaatgctcg gaccggctgg
     5641 accggcgcct ggctcgggcg gccagatccg gttggcgagc gccgtggtga gcagtgcaac
     5701 gatgacggcg atggccggcc cgatgatcca cttggccatc ggcgggatgc cggcattcgg
     5761 agcgctcgat gcaaaggcga ccgcagccgg acgcatggtc aaaggaatgc tggcgttgcg
     5821 ggctcctggc ggaagcgttg ctgccatcga ctgcgggttc ggaatctgcg gcgctggcgt
     5881 cgcatacatc gtcgcttgct gcggacccgt cgccaccaca ggggcacccg gcgggggact
     5941 gggaatctgc ggtgcttgcg cgatcatcgt gcgggccccc tctggcgatg ctcctggggg
     6001 tgctgcggac ggtggcgccc cttgcggtgg caaggacggc gcctgcgcga tcaccgtgcg
     6061 tgcacctgcc gcgggcggcg gcgcgccgcg agcgatggct gcggcaactt ctgggggcat
     6121 ctcagtggcg atttgggtct tttgatcaga tccagacatg ttgctccgcg acgctggctc
     6181 ttgtggagct ggcgtagaaa gagtggataa gccgcctggc tcggcggcag atgtgggtgg
     6241 tggaatcggc ctgtgtggtg tcgctagtgc ggcaatctct gccgggcgaa gctgcgccgt
     6301 ttcgtgtgac tgggcttctg gcggcggtgg cagcgagcgt ttcgggcgtt ttcccgattg
     6361 actcagtgaa tcgggcgctt tggcggctgg ttctgtgccc gacgctgcac tggctggagc
     6421 cgccgtcgga aacgggttcg gaatcgtgct cggcagcggc gtcatcgcca gcatgccttt
     6481 gtcgatgggc cgcttcggct ccttcttgag catgtcttgc aagagcttgg tccagttgcg
     6541 cgactgctcc ggaaacagcg tccccatgaa ctcgccgagc ggctgggcct cgtaggcata
     6601 ctgcggcagc gccatcagct gcctaaccac ttcggcggcg gtctgcgtgc gctctttgcg
     6661 attggcgacc agcatcccga ggacgatttt ttcgacttct tcgggaatgt caggattgta
     6721 ttgccgcggc ggtgggacga tgccgtcgcg aactcgcctc accacatcct gctcgtcggc
     6781 cccaaaaaac agccgatgcc gggtgataag ttcccaaaac acaatcccca tcgaatagat
     6841 gtcgctgcgc gcgtcgagcg cctggccggt gatctgttcc gggctcatgt acgacagctt
     6901 gcctttgacg gttcctccgg cgggcgcggg ctgccgttcc gcgaccttcg cgatgccaaa
     6961 atcgctgatt tttacgtcgc ccgaaaagct gatgaggatg ttgtgcggcg agatgtcacg
     7021 gtgcacgatg ccaagcggct tgccgtggtc gagccgcgtg tgggcgtact gaagcccttt
     7081 gagcgcctca ctgatgatgt agatggaaac gctgagcgga aaccaaatcc cggctttggc
     7141 ggcggccttt tcgctctgac ggagatcgcg tccttcgacc agctccatcg ccaggtaata
     7201 gcgatcgcct tctttgtcga agtcgaagat ttggacgatg ttcggatggt gaagcttggc
     7261 gacgagacgc gcttcatcaa tgaacatccg gatcgattct tcatcgccga ggaacttggg
     7321 caggatgcgc ttgataacga ggtctttggt aaagccttcc gagcccagcg tcttggcgcg
     7381 ccagacttcg gccatgccac ctatgccaat ccgttcgagc aggtgatatt tgccgtatcg
     7441 ttcgggggct tgcggcgccg attcagcaat gatcgatgcc gaggctgtga tcggcgaacg
     7501 tggctcggct gggggacggc tgcgcttggg ttcaggcggg cgacctgcgg gcgtggtcaa
     7561 gcgatcgggc gacggcagag gagccgacgc accatcactg tgcggagggg gcgaggtctt
     7621 cgtcgacacg gaggcagact acaaatgtac cacgggcacg ccgaatgtct gtgatcaatt
     7681 tggttttgag ggacacatat tatctgcaaa aaacgaaaat ggtttcgtgc caactgcaag
     7741 gacggaagtc ccaaagttat caacgggctg cgtggtttgc cgtggcagag tctcattccg
     7801 acggtaatag ttggtctgtg tcgtcgctac atggccggtt gcatggggat ggcggcctag
     7861 catatgatct gcacgttgtt tattggtatt ctccagcacc tagacacgat ctggagaaag
     7921 catccccaag agggtttctc gatgcatcac agtgcaaacg ctactcggct ccgtcaccgc
     7981 tgtactgttc ttttgctgac tgcgtcgctc ggactaggcg gccttgcctc cacgggctgt
     8041 cagacgctga gcaccgcgca aagcggcatg cagaccggcg tctccaccgc gcagaccacc
     8101 aaatcgaccg ccgatcagac caagcagctt ggtaccgatc tgaagggcga agtggacaag
     8161 gcgacgggca aaaagaccga cggggcaggc ggtgccgctg gggataagcc agaggatgaa
     8221 gacggcaagc gtcttcaggc caaaagttcc aagttaaacc agccggtcaa cgacgagcta
     8281 aaccagacca aaaacgatct gaccgattgg aaagcctacg atttgtcggg catctcgtcg
     8341 ggaaaatggg tcgaatttgt cctcaactgg gacgagcctg acgccgaaat caacctggac
     8401 atctacgatc aggttggcgc gcaggtcgtg cagtcgccag gtcgggccgg tgtaccgcaa
     8461 aaaaacgtgc cgctgcgggt tctgacgccg gggatttact acgctcgtct cacgctgcaa
     8521 ggcaactatc ccaaaaaaga gtcggtttat accgtcgtcc tgaagaccgg gaaaaatgcg
     8581 gcgctggcca aaccgacgtc gatttctggc aaaaaagaag gcgacgcggc ggcagcgggt
     8641 gcagcaggcg gagcgccagg ggccccagga gccccaggag ccccaggagc accgggagga
     8701 gcgcccggaa tgggaggagc gcccggaatg ggtggagcgc ctgggatggg cggagcgcct
     8761 ggaatgggcg ggtatcctcc gatgggtgcg atgccaggcg gcatgggcgg ctacccgcag
     8821 attggaatgc agccgggcat gggcatgcag ccgggcatgg gcatgggaat gcagccgggc
     8881 atgcagggcg gagccggtgg agccggtggc gcgggtggag ccggtggcgc gggcggtggc
     8941 gcaggcggcg gtggtggcgg cgattcgtca gccattcccg aaggcgcgat cgtgggtcga
     9001 atcgttcagt ccactgtcga agagggcaag cgcatcctgt atctcgacaa aggcagcaaa
     9061 gccaagctgc gcgtcggtat gcagggcttc attctgcaag gcaaggaagg cgggcagaag
     9121 ctcgacggcg gcaccttcac gattaccaaa gttctcgggg acagccaggc ggttggctcg
     9181 acggactacg gcaagtcgct cggtagcaac aaccgtttca tgatccagaa gccgaagtag
     9241 gcgtgacgag cgcgtcaggc ggaaatcttc gaacactttt ctctttggga cgaggcaaca
     9301 gtgatgcttt tccgagccaa acttctccgg accttgtttg cgtcgagctt gctcatcatg
     9361 gcggcggcgg gctgcaaaaa agacgacgtc aacgcgaaga tcaaaaacgg tcccgatccc
     9421 gataccaaca gcggcgtcga cgccaagcgt ggtggtgcca agccgatgca gataaaccgg
     9481 cccgagactg actcggtgag ctggccgaaa ctgcagcgca ccaagtggaa gagcatcgag
     9541 atagcgacgc cgggcaaaga caacgtcctc gactgcgagc tgcgcatcga caacaccggg
     9601 gctgacctga acatcgactt gttcaacagc cttggaacgc agatcgggtt tacgccgggt
     9661 cctgcgccga acaagctcaa gaagctggcg gtcaagctcg aagagcttgg cacgtacttt
     9721 gttcggatgc aggccgcgat gcccaaagat gagtcggact tttcggtgat ctgtcgctgg
     9781 gaagaagagg cggttgtgcc tgatgcgccc gacaagccga agaaggcggt gaagcggacc
     9841 ccgacggaaa agccagccaa ggccgaaacg ttcgaggaga agatcgaaaa aggcaccgaa
     9901 ggccgcatca agacttcgtt ccgcgagggt ggccagatgg tgatgatcgt cgacaaaggc
     9961 gctgccgcgg gcgtgcacga aggcaccgaa ggcaccgtgc ttgaaggcaa gagcggtcgg
    10021 cagcctctcg acggtggcac cttcaagatc cttcaggtta tcgacgattc gacgagcaag
    10081 gcccagtcgt cggttcgatc gatcggcaag aacagccgcg tggtgttgtt cctcaagtaa
    10141 gaactcgtcg ctcgggtaag gagtcggcag gatgagcacg ccaggccagc aagatccgct
    10201 gattgggaaa gtactcgcgg atcgctttga gatcctgagc cgcatcggtg aaggcgggac
    10261 tggcgtggtc tacaaggcca agcaaattac cgtcgaccgc accgttgcta tcaaggtgct
    10321 cggcgcgcat gtgtcgtcgg acccgcagtg ggtcaaacga ttccacaacg aagcgcgtgc
    10381 agcggcccgt cttgaccatc cgaacaccgt ccggatgatc gactttggcg aaaccaaaga
    10441 agggctgctg tttatcgcca tggaatacct ccatgggcgg cccctgtccg acgaaatttc
    10501 gcggcttggc aagattccgc cccagcgagc cttgcgcatc atttcgcagg tctgtcagtc
    10561 gctgcaagaa gcacacgggc agggcatcat ccaccgggat atcaagccgg acaacgtgtt
    10621 tctcgtcgag atgaagggtt cgggcgactt tgtaaaagtg ctcgactttt ccgtcgcaaa
    10681 aatggatacg cccgacgcgc agctgacgcg ggctggcacg gtgttcggaa c
//
LOCUS       DAORGN010000176        10694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2620_length_10694_cov_7.217220, whole genome shotgun sequence.
ACCESSION   DAORGN010000176 DAORGN010000000
VERSION     DAORGN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2620_length_10694_cov_7.217220"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKL17_16615"
     CDS             complement(<1..234)
                     /locus_tag="PKL17_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46406.1"
                     /translation="MGGGNYDEDIAIEARSSNRDVFTYRGYGSSGESAARRGVHPILE
                     PHGKIRECMNETPIVVAMDVTRSRGDDTRIIYDK"
     gene            complement(257..1471)
                     /locus_tag="PKL17_16620"
     CDS             complement(257..1471)
                     /locus_tag="PKL17_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46407.1"
                     /translation="MLHDDLAEIQSLLSAARGTQDVFPSMALRDVDIDEKQVLLEEEY
                     VVLVRRLRPERFGGNSLAQNSARHLLSKIYDWHQRAVQELEQERAAAEPNHAEADSQR
                     DDSQTDSAAESGYRPPPPRLRSVPNPPADSAVDFTITTQSGSYRATKLLTQGDVAMLY
                     RGVCTSGLRSGAEVTVKIAMESADNDLLLDEARILRTIHSAEGAQRKHLPEIFDQFQT
                     PSGQAGSILSYLDGFDLDEVRERYPDGLNPEHVAWVLMRALSALGHAHKLGIIHGNVE
                     PAHVLVRPSDHNVFVIDWTYSIVAPEKTGQGFRAHNPDFSPPEVLARKAPLPASDLYS
                     LGKTMIYLLGGDVRQATLPPRVDGRFARFIQFMVMESPRQRAQDAWETAEQLAQLRTD
                     VFGPHRFLPLEM"
     gene            1557..2726
                     /locus_tag="PKL17_16625"
     CDS             1557..2726
                     /locus_tag="PKL17_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46408.1"
                     /translation="MRKWAKALGYSRCWAIAKKTEYRIAMHIVRIQRGPQPQVSLLQE
                     HRYEKEKDLERDLASSPLLIQQVMGTPIHLLASSFRLPGDERVAGLYIDSDGRLLICE
                     ARLARNLGSAREVLGQLLDHVSALSQYRIENLSAALSGKLEEVLRTISRGDIDRYTAL
                     WQQVSDHLTAGRVGMALVLDRAPAHLQRICRYLCQNVRIQFVLLTLKRFRASDDSDII
                     VPTLIAQAAQQQAQANLLELAVQYAHQGPAAIQIEHIKHRGNSLAIHPRGWPTAVRYE
                     FLIHPEQDCRIGIELRIDDRDMPQARDLIRVLGSSDILEVLKIHFDSARIIWEAAQSP
                     VGLAILFDAASEPEALSSAMSVLMELTYARVTERLRHLVRPPESQPKPASGKEES"
     gene            complement(2777..3706)
                     /locus_tag="PKL17_16630"
     CDS             complement(2777..3706)
                     /locus_tag="PKL17_16630"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNK46409.1"
                     /translation="MRSPQTKATSSRSSSLFSRRWAGAALIASFQLLAQNQAQALPVG
                     NPQEAAEAESKLPTWDEIDAAAVDFASIVALSNCSGSLVRFSTSKPDDYALVLTNGHC
                     LSGFVPAGDAVVDQASSRTFTLLDASARSLATLRAQRMMYATMTGSDFAIYKLTQTYA
                     QLSSRYRVTALTISSRRPGEGTPIRIVSGYWRKIYSCSIDAFVPELREGTWTFRDSIR
                     FKQPGCETIGGTSGSPIIDAETRAVIGINNTGNESGATCTLNNPCEVDASGKITATKG
                     AAYGQQLYQVYACLDASNNIDLNRAGCTLQKPK"
     gene            complement(3838..5949)
                     /locus_tag="PKL17_16635"
     CDS             complement(3838..5949)
                     /locus_tag="PKL17_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK46410.1"
                     /translation="MSPCSPFPWTWRRAASLVIPPQCSVAGICAWLCCALPMAHAESS
                     FDVVVRAEDPALRAEVSKQTLHTEELRKMPGTLGDPLRAIENLPGAARPPLNTGLFII
                     EGARPSDSRVYLATAEVPQLYHYGGFTSVVPADFLSSVTYLPHNFGVRYGRATAGTID
                     VDLRAGKTDRWHAALDVNPIHIGVEVEGPLPPRNAQTQQPSGSILLGLRRSYVDAGLA
                     FVGAVLGDPPGLRFVNAPVYWDYQAVLKYALAGGTLRVMVLGADDQISLKFARPQDVD
                     PSVNGTFATHILFHRLQLRYQRQFSGWDVLFQSTSGYTQTDLDLGRTVSMSVKTLGND
                     FRVEASRQITPQYRLFFGTDVQTSYVWLSAAVPSPVREGEFQKPPGSLSQVVAQAEAY
                     FVNPALYAELSAQLHPRVSVVSGVRFDYFGYLRRYSIDPRLSGKVMLTPITQLKWGAG
                     LYSQDPAVQDYFSSFGNRELRLERAVHLSLAVEQAIWKGLVLELTGLYKYLWDFAGTS
                     TLSRRFGGGENPLELQPERTASQVHGRIFGGTLLLRQSLSKYFFGWVSYSLLRSERAD
                     CPSCSYRLFDFDQTHILIVAAHAYLPYKFEIGLRFRYISGIPQTPVRGGFFDSDTDLY
                     QPIPASERNTDRLAPFHQLDLRIDRTFVTKKMVFKLYLDVSNVYNSPAQEQLVYSYDY
                     SQRAAITGLPILPSLGVRGEL"
     gene            6556..8211
                     /locus_tag="PKL17_16640"
     CDS             6556..8211
                     /locus_tag="PKL17_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46411.1"
                     /translation="MSRPLLTRTLSGAMLALSLGACTAPQVGSPCPIPTNADSATRLA
                     AVQKCFGLIAQDHNDYSLKKDVDILFMIDNSPSMSPKQKALADNIPKFIQSIESFGVN
                     YHVGIITSDVGTTVSDGATWGGGSEPACDTFSGDDGRLQDITCDKRNTVSPDARSACA
                     TLCPDNRFRPTDGSKFISKIDGKTNVPQDVRPDPMTGAMVDYGPSNAFKCMALVGDAG
                     CGVEGPLEAVKRALTTNSTTANANFLRSGSLLAVIFITDEDDCSVKASRRSELNPNYR
                     NCTPGQPDSYDCYKVDQRCLLRSLQCNEPMSTPGGKTGCVERPDNFLEPVKTYFDALR
                     QIRPDDDKLLISGIWTQPNIDTAKGGTVGIVGGPLTSDLKRDPACKNATDTTIVGQPQ
                     FRLSQLATMYGVGKDGSANAPQLNVCNTADYPTALKQIAKAIEVKLNPCLPVNVKTIN
                     GAAICLVGDVDASTPDAVPTTYFPQCSNTCCDAWASSSTPSLADKTIQAACAAETTDA
                     CFCAVKPKDPTVCKDGGTVTAVWRKGGVNTPLGKTVNFRCAGF"
     gene            8448..10115
                     /locus_tag="PKL17_16645"
     CDS             8448..10115
                     /locus_tag="PKL17_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46412.1"
                     /translation="MRRSQLPFALAGLGSALLMSACSSPQVGSTCPVPTTGDKASKMK
                     EFSKCFSQLGESVVDARLRKDVDILFLVDNSPSMTPKQKALANNIPKFIQKIDATGAN
                     YHVGIATSDVGSNVQPGVPWGGNIGKCDSYEGDDGVLQAVPCTTRNTVTPDARNACAQ
                     LCPDDKFVPTDGRRYISKVDGVTNVPVSMEKDPVSGKMVDVGPNKAFKCMALVGDDGC
                     GIEGQMEGAKRALDGHRSENSGFLRPNSVLAVIFITDEDDCSVQMAKRDQNNPSYRDC
                     DPTQPDSPECYNVDFRCLARSIQCNETTLTTGVKTGCKERANNYLEPVEKYYRFFSNL
                     RPANKLLVSGIWTLPSIDNGGRVEVARVGGTTTPNLNRAGGAGASCAYAGDQSVFGQA
                     QLRLSKFAKQFGTDAKGQANALEVSICDIDNYPTALDRIAKAIEQRLQAQCLPVTPKT
                     DASGNPVCIVGDVDEASPASAPDPDTAFPVCSKSCCGAWAQTSTPTPGDDTIKAACAA
                     ESRDCYCAIKSKDPSICTESVVAGVWRKGGAQPPPGKVVNFRCAGGG"
     gene            10289..>10694
                     /locus_tag="PKL17_16650"
     CDS             10289..>10694
                     /locus_tag="PKL17_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46413.1"
                     /translation="MNERGSRKIPVTERQFLWVQDDDKGEVTLHVGPTMVSPTAADRV
                     VIDDGVGSFREDTSGRPQMMIEVGDHQYAVLFNPLLENDGGPNGRFRVGRNEARPLKN
                     GTRAMIPGPCSFFLRPGQRCEVRDAHELSASQY"
BASE COUNT         2309 a         3335 c         3034 g         2016 t
ORIGIN      
        1 cttgtcatag atgatgcggg tgtcgtcgcc tcttgatcgg gttacgtcca tcgccaccac
       61 gatcggcgtt tcgttcatgc actcgcggat ttttccgtgc ggttccaaga tcggatgcac
      121 acctcggcgc gcagcagact cccctgacga accatagccg cgataggtaa acacatcgcg
      181 gttggacgat cgcgcttcga tcgcaatgtc ctcatcataa ttgccaccac ccatgggttc
      241 ctccctctgc tttctgctac atctcaagcg gcaggaagcg atgcggacca aatacgtctg
      301 tccggagctg cgcaagctgc tctgcggttt cccacgcatc ttgcgcacgc tgccgcggac
      361 tttccatcac cataaactga atgaaacgag caaagcgccc atcgacgcgc ggaggcagcg
      421 ttgcttggcg aacatcgcct cccaaaagat agatcattgt cttgccgagc gagtacagat
      481 ccgatgctgg aagcggcgct ttgcgggcca agacttccgg cggactgaag tcaggattgt
      541 gcgcgcggaa cccctgcccg gtcttttccg gcgcaacaat ggagtatgtc cagtcgatca
      601 caaagacatt gtgatcgctt ggccgaacca gcacatgcgc aggctcgacg ttgccgtgaa
      661 tgattcccag tttgtgggca tgacccagcg cggacagagc ccgcatcagc acccaggcaa
      721 cgtgttccgg attgagtccg tccgggtaac gctcgcgcac ttcatccaga tcgaaaccat
      781 cgagatagga caagatcgat ccggcctgac ccgacggtgt ttggaactga tcgaatatct
      841 ctggcagatg cttgcgctgc gcgccttccg cactgtggat tgtccgcaag atccgcgctt
      901 catctagcag cagatcattg tccgcgctct ccatcgcaat cttcacggtg acttccgcac
      961 cgctgcgcaa tcctgaagtg cagacgccgc gatacagcat cgcgacatcc ccttgggtca
     1021 gcaatttggt cgcgcgatag cttccgctct gcgtcgtgat cgtaaagtcc acagccgaat
     1081 ccgctggcgg atttggaaca ctgcggagac gcggcggcgg tggacggtat cccgattccg
     1141 cagcgctgtc tgtttgggaa tcatctcttt gagagtctgc ttccgcatgg ttcggttccg
     1201 cagcagcacg ctcctgttct agctcctgca ctgcccgctg atgccagtcg tagattttgc
     1261 tgagcaggtg tcgcgcactg ttttgggcca ggctgtttcc gccaaatcgt tcaggacgca
     1321 gccttcgcac cagcacgaca tattcctctt ccaacagaac ctgtttttcg tcgatgtcca
     1381 cgtcgcgcag cgccatcgat ggaaagacat cttgggttcc gcgagccgcc gatagcaggc
     1441 tttgaatctc ggccaaatcg tcgtgtagca caacgccccc tctcgatgta caggcagtcg
     1501 cccaaagcga ccgccgacta cgaggatagg acccccgaaa gccctaagaa agcggcttgc
     1561 gcaagtgggc gaaagcactg ggttattcac gctgctgggc catcgcgaaa aagaccgagt
     1621 accgtattgc gatgcacatt gtgcgcattc agcgtgggcc ccagccccag gtgtcccttc
     1681 tgcaagaaca tcgctacgaa aaggaaaaag acctggagcg cgatctggct tcgtccccgc
     1741 tcttgattca gcaagtgatg ggaacgccga tccacctgct cgcgtcttcc ttccgcttgc
     1801 ctggtgatga acgggtcgcc ggtctgtata tcgacagcga cggaagattg cttatctgtg
     1861 aagcccggct tgcacgcaac ctgggctctg cacgcgaagt gctcggacag cttctcgatc
     1921 atgtctctgc actgtctcaa taccgaatcg aaaacctcag tgccgcactg tccggaaaac
     1981 tagaagaagt gctgcgcacg ataagccgcg gcgacatcga tcgctacacc gcactgtggc
     2041 aacaggtcag cgatcacctc acggcaggac gggtcggaat ggcgctggtg cttgatcgcg
     2101 cgccggccca tctgcagcga atctgtcgtt atctgtgcca aaacgtcagg attcagtttg
     2161 tgctgctcac actcaaacga ttccgggcaa gcgatgacag cgacatcatt gttccgacgc
     2221 tgattgcgca agcagcgcag caacaagcgc aggccaacct gctggaactg gcggttcagt
     2281 acgcccacca aggaccggct gcgatacaga tcgaacacat caaacaccgc ggcaattcct
     2341 tggcgatcca cccacgcggc tggccgactg cggtccgata tgaattcctg atccatccag
     2401 agcaggactg tcgcatcggc atcgagcttc gcatcgacga tcgcgacatg ccgcaagcgc
     2461 gcgatcttat ccgggtgttg ggatcgtcgg atattttgga agtgttaaag attcactttg
     2521 acagcgcgcg gatcatctgg gaagcggcgc agagcccagt cggacttgcc atcttgtttg
     2581 atgcagcgag cgagcccgaa gcgctgtctt ccgcgatgtc ggtgctgatg gaactgacct
     2641 acgcccgagt gaccgagcga ctgcggcacc tagtccgccc gcccgaatca caaccaaagc
     2701 cggccagtgg aaaagaggaa tcctaaccac ggaaggggga tctccttttg aacaaaagga
     2761 gtccttacac gctcgctcac ttcggttttt gcagcgtgca gccggcgcgg tttagatcga
     2821 tgttgttgct tgcatcaaga cacgcataca cctggtaaag ctgctgtccg taggcagcgc
     2881 ctttcgtcgc tgtgatcttg ccgctggcat ccacttcgca tggattgttg agcgtgcaag
     2941 ttgcgccgct ttcgttgccg gtgttgttga ttccgatcac ggcgcgcgtc tctgcatcaa
     3001 tgatcgggga accggatgta ccaccgatcg tttcgcagcc cggctgcttg aaacggatcg
     3061 agtcacggaa cgtccaggtg ccttcgcgca actctggaac aaacgcatca atcgagcagg
     3121 aataaatctt gcgccaatat ccggagacaa tccggatcgg ggttccttct cccggacggc
     3181 gggacgaaat cgtcagcgcg gtcacgcgat aacgcgacga aagctgggca tacgtctgcg
     3241 tcagcttgta gatcgcgaag tccgatccag tcatcgtcgc atacatcatc cgctgtgccc
     3301 gcagcgtggc caacgatcgc gcactggcat ccagcagcgt gaacgtccgg ctcgacgctt
     3361 gatccaccac cgcatcaccg gcaggaacaa agccgctcaa gcagtgacca ttggtgagca
     3421 ccagcgcata gtcatcgggc ttcgacgtac taaaacggac cagcgatccc gaacagttgg
     3481 aaagcgccac aatcgacgcg aagtccaccg cggctgcatc gatctcatcc caggttggca
     3541 gcttggattc tgcttcggcg gcttcctgcg gattcccaac cggcagtgct tgcgcctggt
     3601 tttgcgccaa aagctgaaac gaagcgatca gcgcggcacc agcccagcga cgtgaaaaca
     3661 gggatgaact acgcgacgaa gttgcctttg tttgtggact acgcatcctc tctcctcctt
     3721 gctgtttgtg aaattcgcaa gcgccaaaca ctcatcgctt aggaatgtgc aagcagacaa
     3781 gatgggagtt ttcttcccga ttcgcaggat ggctggggac ttcaccccca tgcacatcta
     3841 cagctcccca cggactccca acgaaggcag aatcggtagt cccgtgatcg cggcgcgctg
     3901 ggagtaatca taggaataga caagctgctc ctgcgccgga ctattgtaca catttgacac
     3961 gtcaagatac agcttgaaca ccattttttt cgtcacaaac gtccgatcaa ttcgcagatc
     4021 aagctgatga aacggagcca gtctgtctgt attccgttcc gacgctggaa tcggctgata
     4081 cagatcggtg tcgctgtcga aaaaaccgcc gcgcaccgga gtctggggaa ttccacttat
     4141 gtaccggaaa cgcagaccga tctcgaactt gtagggcagg taagcgtgcg cagcgacaat
     4201 caggatgtgg gtctgatcaa agtcaaacaa gcgataggaa cacgacggac aatcggctct
     4261 ctcacttcgc aagagcgaat agcttaccca accaaaaaag tacttcgaca ggctctgccg
     4321 caagagcagc gtgccaccga aaatccgtcc atgaacctgc gaagcagtcc gttcaggctg
     4381 cagctcgagc ggattttccc caccaccaaa acgccgcgac agcgtcgatg ttccggcaaa
     4441 atcccacagg tatttgtaaa gcccggtaag ctccagaacc agtcctttcc agatcgcttg
     4501 ttcgaccgcc aaagacagat gcaccgcgcg ttcaagccgc agctcccgat tcccaaacga
     4561 tgaaaagtag tcctgcactg cggggtcttg ggaatacagt ccggcgcccc acttcagctg
     4621 tgtgatcggt gtcagcatca cttttccgga aagccgcgga tcgatcgaat agcgacgcag
     4681 ataaccaaaa taatcaaagc gcacgccaga cacgacagag acccgcgggt gaagctgcgc
     4741 cgatagctcg gcatagagcg cgggattcac aaagtatgct tcggcctgcg caacgacttg
     4801 ggacagagat ccgggcggtt tctgaaactc gccttcgcgc acaggcgatg gcaccgccgc
     4861 agacagccag acataggaag tctgcacatc ggttccgaaa aacaggcgat attgtggagt
     4921 aatttgacgg ctggcttcga cgcgaaagtc gttccccaac gtctttaccg acatcgaaac
     4981 cgtgcgcccc agatcaaggt ctgtctgggt gtatccgctg gtgctttgga acagtacgtc
     5041 ccagccggaa aactgtcttt gatagcgcag ttgcaagcgg tgaaacagaa tgtgggttgc
     5101 aaacgtcccg ttcaccgagg gatcgacatc ctgcggacgc gcaaacttga gcgagatttg
     5161 atcgtccgca ccgagcacca tcacccgcag cgtcccgcca gcgagcgcgt actttaagac
     5221 cgcttggtag tcccaataga ccggcgcatt cacaaagcgc agccccggcg gatcacccag
     5281 cacagcccca acaaacgcca gccccgcatc cacatagctg cgccgcagcc cgagcaaaat
     5341 cgatccgctc ggctgctgcg tctgcgcgtt ccttggtggc agcggacctt cgacttcgac
     5401 cccaatgtgg atcggattta catcgagcgc agcatgccag cgatcggttt tcccggctcg
     5461 cagatcgacg tcaatggtgc ccgcggtcgc ccgtccgtaa cgcacgccga agttgtgggg
     5521 aagatacgtc acggacgata aaaaatccgc cggcaccacc gatgtaaagc cgccatagtg
     5581 gtagagctgc ggcacctcgg cggtcgccaa atagacgcga gaatccgacg gacgcgcgcc
     5641 ttcgataatg aaaagtccgg tgttcagcgg tggccgcgcc gcccccggca agttctcgat
     5701 ggcacgcagc ggatcgccga gcgttccggg catcttgcgc agctcttcgg tgtggagcgt
     5761 ctgcttgctg acttccgcac gcagcgccgg atcttcggcc cgcacgacca cgtcaaagga
     5821 cgactccgca tgggccatcg gcaaagcaca gcacagccag gcgcagatgc cagcgacgct
     5881 gcattggggc ggaatcacaa gcgacgccgc ccgacgccac gtccaaggaa acggcgaaca
     5941 agggctcatg acccacacag cctaccactc ggatgcggcg gcgggtctgc agcgaatcgg
     6001 cgcgctggcc ggaacgcggt cctagcggcg ggcctttttc ttgggtcgcg gcggcggact
     6061 gggctgcgcc cccggatatc ccacgagcca cgtcggacat gtcagcacag gagtcatacc
     6121 ccccagaacg cagaacgggg cgcgaagttt tttccgattt tgacttgttt tttgtttggc
     6181 atgttacatg caactaacaa agggtattgc gcaccggaac cacccgaccg ataaacatgt
     6241 gtccggcctt gtcagatcca agctcgcttc aagcgattgg tttgtcgaat gggttgaaag
     6301 gatcgcgcag cggcccggcc tagacacacg gggttttagc tgaaccgatc ggatgggatc
     6361 agcggattga gcagcccgat gcaacaaaaa aattttgtgt tttggaacgc aaaactcgca
     6421 agtatgctaa agatcggtta aaaacaaacg agcgcccgtg ccagcaggag ccgggagcca
     6481 ctcagggggg acgtttcttt gcagcgggcg cttcgcgcgc cgtcccgcct gaatacagaa
     6541 cacagggagg aatcaatgag ccgtccactt ctcacgcgca cgctgtccgg ggccatgctt
     6601 gccctgtcgc tcggcgcgtg caccgcgcca caggttggca gcccttgtcc gattccgacg
     6661 aacgccgaca gtgcgacgcg gctcgcggcg gtccaaaagt gctttggtct catcgcccag
     6721 gaccacaatg actacagcct gaaaaaagat gtcgacatcc tgtttatgat cgacaactcg
     6781 ccgtcgatgt cgcccaagca gaaggcgctt gccgacaaca tcccgaagtt cattcagtcg
     6841 atcgagtcgt tcggcgtaaa ctaccacgtc ggcatcatca cgtctgacgt cggcacgacg
     6901 gtatcagacg gcgcgacgtg gggcggcggc tccgaaccag cctgcgatac gtttagcggc
     6961 gacgatggac ggctccagga catcacctgc gacaagcgca acaccgtatc gcccgacgct
     7021 cgcagcgctt gcgcgacgct gtgtcccgac aaccgtttcc ggcccaccga cggcagcaag
     7081 ttcatctcga agatcgacgg caagaccaac gtgccgcagg acgttcgtcc tgatccgatg
     7141 accggcgcaa tggtcgacta cggcccgtcg aatgcattta agtgcatggc gctcgtcggc
     7201 gatgctggct gcggtgtcga aggcccgctc gaagcggtca agcgcgcgct caccaccaat
     7261 tcgacgacgg cgaatgcaaa cttcctgcgc agtggatcgc tgctcgctgt gattttcatt
     7321 accgacgaag acgactgctc ggtcaaggcg tctcgtcgca gcgagctaaa ccccaactac
     7381 cgcaactgca ccccaggcca gcccgacagc tacgactgct acaaagtcga tcagcgctgt
     7441 cttttgcgct cgcttcagtg caacgaaccg atgagcacgc cgggtggcaa gaccggctgc
     7501 gtcgagcgcc cggacaactt cctggagccg gtgaagacct actttgacgc gctgcgccag
     7561 attcgccccg acgatgacaa gctgctgatc agcggcattt ggacccagcc aaacatcgac
     7621 accgccaagg gcggcacggt cggcattgtc ggtggtccgc tgacgtcgga tctcaagcgc
     7681 gacccagcct gcaaaaatgc cacggacacg accatcgtcg gtcagccgca gttccgcctg
     7741 tcgcagcttg cgacgatgta cggtgtaggc aaagacggaa gtgcaaacgc gccgcagctc
     7801 aacgtctgca acactgcgga ctatccgacg gcgctcaagc agattgcaaa ggcgattgag
     7861 gtcaagctca atccgtgtct acccgtcaat gtgaagacga tcaatggcgc agcgatctgc
     7921 ttggtcggtg atgtcgacgc gagcacgcct gacgcagtgc ccacgacgta cttcccgcag
     7981 tgctcgaaca cctgctgcga tgcctgggcc agcagttcga cgccgtctct cgcggacaag
     8041 acgatccaag cggcctgcgc tgccgagacc accgacgcct gcttctgcgc ggtcaagccc
     8101 aaagatccga cggtctgcaa agacggcggc accgtgaccg ccgtgtggcg caagggcggc
     8161 gtcaacactc cgctcggcaa gaccgtgaac ttccgctgcg ccggcttcta gtccccatgg
     8221 gcagccgcta acgactgccc cacccgcctg atctccccgt cgtcacgcta caaattccgc
     8281 aaaaacgcag tccttccgta aggttttctt gcgtccgtgg cctaacccac caagtagtcc
     8341 attggcggtg actgtcgctt gctctccccc gttcgatcag gtacggtgcc gccgaacgcc
     8401 aacgacttac acggtttgcg accccgaaat tcgcacggag gcttgtgatg cgacgttccc
     8461 aactaccttt cgcgctcgct gggctgggct ctgcgctgct tatgtcggcc tgctcatccc
     8521 cccaggtcgg ctcgacgtgt ccggtgccca cgaccggtga caaagcgtcg aagatgaaag
     8581 aattcagcaa gtgcttcagc cagctcggtg agtctgtcgt cgacgcacgg ctgcgcaagg
     8641 atgtcgacat cctgttcctg gtggataact caccgtcgat gacgccgaaa caaaaggcgc
     8701 tcgcgaacaa catcccgaag ttcattcaga agatcgacgc caccggggca aactaccacg
     8761 tcggtatcgc caccagcgac gtcggcagca acgtgcagcc gggcgttccg tggggcggca
     8821 acatcggcaa gtgcgacagc tacgaaggtg acgacggcgt gcttcaggcc gttccctgca
     8881 ccacccgcaa caccgtgacc cccgatgcgc gcaacgcttg tgcgcagctc tgccccgacg
     8941 acaaatttgt ccccaccgat ggtcgccgct acatctcgaa agtggatggc gtgaccaacg
     9001 tgccggtgtc gatggaaaaa gacccggtct ccggcaagat ggtcgacgtt ggcccgaaca
     9061 aagcctttaa gtgcatggcg ctcgtcggtg acgatggctg cggcatcgaa ggccaaatgg
     9121 aaggcgccaa gcgggcactc gacggacacc gcagcgaaaa ctcgggcttt ttgcggccca
     9181 actctgtgct cgcggtcatc ttcatcaccg acgaagacga ctgctcggtt cagatggcga
     9241 agcgcgatca aaacaacccg agctaccgcg actgcgatcc gacgcagccc gactcccccg
     9301 agtgctacaa cgtcgacttc cgctgcctgg ctcgatcgat ccagtgcaac gaaacgacgc
     9361 tcaccacggg cgtcaagacc ggctgcaagg aacgcgcgaa caactacctg gagcccgtcg
     9421 agaagtacta tcgattcttt agcaacctgc gcccggccaa caagctgctc gtcagtggca
     9481 tctggacgct gccgtcgatc gacaatggtg gccgcgtcga agtggcccgc gtcggtggca
     9541 ccaccacgcc aaacctaaac cgtgctggcg gcgcgggtgc atcgtgcgcg tatgccggcg
     9601 accagagcgt cttcggccaa gcacagctgc ggctgtcgaa gttcgccaag cagtttggaa
     9661 ccgacgccaa gggccaggcc aacgcgctcg aagtctcgat ctgcgacatc gacaactatc
     9721 cgacggcact tgatcgcatc gccaaggcga tcgagcagcg tctgcaggcc cagtgcttgc
     9781 cggtcacgcc gaagacggat gcgtcgggca acccggtctg catcgtcggc gatgttgatg
     9841 aagcatcacc tgccagcgcg cccgatcccg atacggcatt cccggtctgc tcgaagagct
     9901 gctgcggtgc gtgggcccag accagcaccc cgacccccgg cgacgacacc atcaaggctg
     9961 cctgcgccgc ggaatcgcgt gactgctact gcgcgatcaa gagcaaagac cccagcatct
    10021 gcacggaaag cgtcgtcgct ggtgtgtggc gcaagggcgg agcccagcca cctccgggca
    10081 aggtcgtaaa cttccgctgc gccggtggcg gctgatccgc gctttcgtat agccccctcc
    10141 cctgcccgtt ctttcgcgtt ccccccacaa tcccagaccg ccgcaccgtc ttgctgagac
    10201 ggtgaaggct gcgcttgtgc actgcggttg acccgatctg ccgcccgcgc gatgatccga
    10261 ccggattttc ctcgggggga ggcagaagat gaacgagcgt ggcagtcgca agattccggt
    10321 aacggaacga cagtttctgt gggttcagga cgacgacaaa ggcgaagtaa cgctgcatgt
    10381 cggtccgacg atggtgtcgc caaccgctgc cgatcgtgtg gtgatcgacg acggggtagg
    10441 cagcttccgc gaggacacat cgggccgccc gcagatgatg atcgaggtcg gcgatcacca
    10501 atacgcggtg ctgttcaatc cgctgctcga aaacgatggc gggcccaatg gccgcttccg
    10561 cgtcggtcgc aatgaagcgc gacctttaaa aaacggcacg cgcgcgatga ttccggggcc
    10621 gtgtagtttc tttctgcgtc cgggtcagcg ctgcgaagtc cgcgacgccc acgagctgtc
    10681 ggccagccaa tatc
//
LOCUS       DAORGN010000177        10579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2668_length_10579_cov_5.312429, whole genome shotgun sequence.
ACCESSION   DAORGN010000177 DAORGN010000000
VERSION     DAORGN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_10579_cov_5.312429"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..533)
                     /locus_tag="PKL17_16655"
     CDS             complement(75..533)
                     /locus_tag="PKL17_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46414.1"
                     /translation="MRGSIADTLLIDSGFWLALFDTRDPYHNQAICLYPIIEKCHALL
                     PWPILYEVISTRLVKSTDSLNRLYLELQTPGLHRVPDDPYRESALEQAITTANQSRRT
                     SLVDAVLRKILADPGRRIDAMVTFNDRDFADVCARRRIPMYPRDQDWVTP"
     gene            complement(523..855)
                     /locus_tag="PKL17_16660"
     CDS             complement(523..855)
                     /locus_tag="PKL17_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46415.1"
                     /translation="MSKARFWLSYDLGINGDYDGLYRWLDSHGAKECGSSMAYISNYE
                     YEGTLDKAIKGSLSQAVQISKRSRIYLVLKLPGTDAPKGLFLFGGRRPAPWDGYAPSA
                     SGEESDEG"
     gene            complement(978..1184)
                     /locus_tag="PKL17_16665"
     CDS             complement(978..1184)
                     /locus_tag="PKL17_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46416.1"
                     /translation="MTCDVSLLTRDVSLLTRDVSLLTYNASTLTCNVSVVTCDVPAVT
                     CDVPAVTCHPVKQTFDVSKKRLST"
     gene            1322..1867
                     /locus_tag="PKL17_16670"
     CDS             1322..1867
                     /locus_tag="PKL17_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46417.1"
                     /translation="MAKPSTKNESVFDLPLAELTQRVEKAMSLLAEAQALLPGLRTLT
                     EDDRRHSGGKFRTGESEVLHRVLDAIRAQPAYFASLSDEDDGNDPTQLEVDLIADRLA
                     RRDLLSQLQAKFAAVEQPLSDSVLHLGEQTRGVLLAAYRIARTVARTDRKLRDQIAPV
                     IDFYGAMVRRPKATEDKPQPV"
     gene            1888..4599
                     /locus_tag="PKL17_16675"
     CDS             1888..4599
                     /locus_tag="PKL17_16675"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNK46418.1"
                     /translation="MHSSPSSSSIAYPIVATEEPPWLAPFVARLGADKVSTASQDLHA
                     YRRDMWARELLAVAAAQPAGVLPAAVVWPSSTDELQAVVELALAHHVSLTPFGAGSGV
                     TGGATPSAGSLVVDCKRLRSFSLDRDRMTVRCGAGWNGWHIEQAVNRHGLSIGHFPSS
                     IMCSTVGGWVVTRGAGQMSTKYGKIEDMVAALQLVTGRGEKLELDCRQAGPDWVQLVV
                     GSEGRLGFVSEVELQLVPMPQKRLLRGYWFPDVRSGCAAIQQVLQLGLRPSVVRLYDE
                     IDTLIAGTGQHKSSLNQPLFANLSGTAGRFLGQLSGLLGKFELGRSGRPLDLNDLLEL
                     LRPDAERARHRLERWVLQTALQKTEPIGTLLDSVLARLSVGCLLIMGCEGEPATATME
                     EQLVRDEVIRLGGRDLGPEPGEHWLRHRYDVSFKWPRVFAAGAMADTMEFAATWDRLP
                     SLYQKVRAAILPHALIMAHFSHAYPDGCSIYFTFMTRASARGSGPQYAQARVLDDQRR
                     YDEIWQAGIAAALSAGATIGHHHGVGRLRAPFLVDEQGEGLAGLVALGKTLDPSGLCN
                     EGRLFSRDVQLSPKPVVLAAPRAVSVNAQSLLVTAPADLSLRELEQKLRQKELTLGGL
                     PPWAYARTLGDALRSPLPSEACLSSGRLHDRRVQLLLARRDGAGGSLLAIPPQPAPRR
                     STGPDLGQLFVDGLCPNTCQIVGATLRVERFLAARPALTCGFDDWNEALSVLWMARRW
                     HGGTGLHSIQLFSRALIDSVLPQADLPPGKLLLLVEGGGPLSLASESLRELSHRLGMK
                     AGARRGQVLRDLWSPQLDGAGSPFLALGPLHEQHVSPQELAALGPLLLQPNSRWLVAG
                     VHRHGVALASPDALPVSAPRSFPLLEQVAAALDEAYPPLSRPRGAQE"
     gene            4604..5695
                     /locus_tag="PKL17_16680"
     CDS             4604..5695
                     /locus_tag="PKL17_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNK46419.1"
                     /translation="MPDLSTSLELCSLCPKLCSHVCPVYRASAKETLSPQAKMSSLLR
                     LRQSGDAAQPADALPLYACTACGACATACLHKIEPAQHLVRGRALVERRELGHPALSD
                     LPERVFHRAQETSRALLREPDLLSRLAKPGEIGLLPSCLPRRAAGGGPVSEAKQLLSV
                     LDALRSHRADFPQYGLLTVGSAGYPLYAAGLDESFRLYAESLAPKVAELSALVTTCSA
                     CTYLLKVVYPQHGVPLQPKVLHLSELLVPYVTALPIRKRLPKVRYHAPCHLERRLQID
                     APRHLLARLADVVEDVTPAGEDGLCCGAGGLLPQTDPALARAMAHESMAHVEELVPTV
                     SGCPTCGYHLSQSGHPVRDLLDVLCDAIG"
     gene            5803..7275
                     /locus_tag="PKL17_16685"
     CDS             5803..7275
                     /locus_tag="PKL17_16685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46420.1"
                     /translation="MFCCTDLFLPRWLPALGLLLLTACHGSKPTVFPSPLPTQPSATV
                     REIAERFDVVCPPGPHGARVASVISPRFGLPVFSVPSASFPIELLARGPKPALVAALA
                     APSLDDQAAQRCLSAAPGSQCVPLVLADVSSTDIAGTGFATLRATATAAGVGVGGWDL
                     VVQSGSDPPERLHRSVWIEPSDPSLPGPLRIVHLSDLHLGKHPGTTDGLLGSLKAAIQ
                     RVNAEKPDLVVLTGDVVEDGLQEEWHKRAKTELLAITAPLAIVLGNHDYAHFPKIRSP
                     DKAPDGYFAFSREFHSLRRYGFAYHGWDFIGFDSGPSVFAVRVLTRGVDKETVGWLGQ
                     RVEDAANHGRKGVVLFSHAPTRTAPVHAPDSEVSGLCGHMLEGRQQIEQIIETGAERG
                     LRMVHLSGHTHWLELHELRPQARGGEDRWQKRDDGNVCGVVPGGALLLNSPSATQVTF
                     HTVARGNRSGFGVLKLDGDKPVVETLLFDRAGSGGKCSLP"
     gene            7369..7623
                     /locus_tag="PKL17_16690"
     CDS             7369..7623
                     /locus_tag="PKL17_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46421.1"
                     /translation="MSLSKHMRDLVSHRVTFDYENGCHITGYVATVRPAEGEVLTIVL
                     SHVEVSNDAGDVIERHREMVVVPSVLVNYRQTEGPSARQR"
     gene            7627..8352
                     /locus_tag="PKL17_16695"
     CDS             7627..8352
                     /locus_tag="PKL17_16695"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNK46422.1"
                     /translation="MHAAPPSGDLRLLLFSHVVCRPESELDLGQAALLIAEPEYPDLD
                     IAHYLGQIDLLAARLSAQLSTTATNGERAQQLIELLCEDGFRCDPGDYDDPQASFLNY
                     VLDERHGLPILLSVVALEVARRCAVPLSGVAFPGHFLLRGESEKGAPPLLFDPTTGEA
                     LEHADLHALIVRATGKQRQIHKSDIEPAPRHAILARMLGNLRNIYLRHSDVPRLQLAA
                     ERLRLLDTHLPTSQGRPRPSQFH"
     gene            8352..9431
                     /locus_tag="PKL17_16700"
     CDS             8352..9431
                     /locus_tag="PKL17_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46423.1"
                     /translation="MRSFLPLVFALSLLACQKAPPAPRVPSAQEVSAEQVIASYAPSR
                     LRVFPTSSGDYVLWGSERPGLPAVFRPLGKTDKRGPAGIMSRAGELSFVGERMIEAPL
                     PTSPAKTSASLPLLVYDRDAKTAQVQTVTLPGPCTMRDRALIASDGQELFVLARCAIE
                     DQAMVLRLDAALLVRAARTIDGAGAVELLLHHGDSDYLLQARQVLRVPPQGLPVIGTV
                     PPPGGDAETRDLVMAGDLLLVVDGAAGRVIGMDRLSMGWKLEKRFYIDGAVQRLRACA
                     SPNRLQIVLAERTDAGRTELIGLGLSLQMGRRDSGMPQRLLLGGGPSTSDHELLPIAS
                     GDEAMLLRTHDGNTGPQVALSYLHM"
     gene            9486..10289
                     /locus_tag="PKL17_16705"
     CDS             9486..10289
                     /locus_tag="PKL17_16705"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNK46424.1"
                     /translation="MLLLGFLISLFIAYGAHRRGSLTQSGALWAAIVGTVVFGFGDGI
                     AGVALVGFFLSSTWLGKLRRGETAESSDADAVIEKGDCRDGLQVLANGGPSAVFCTLY
                     ALTGKTMYLVGGLASLAAANADTWATEIGLRSPSLPRHILTLEEVPAGTSGAITATGT
                     LGMVAGAVFIGLLTYLEPGRGSVLRTLLVIFGGIFGGWMDSVLGATVQEKRQCVKCGE
                     ATEQSRHICGGATLVTEGIAGIDNDTVNALATAIGGAAAAVAWHFLVGK"
     gene            complement(10305..>10579)
                     /locus_tag="PKL17_16710"
     CDS             complement(10305..>10579)
                     /locus_tag="PKL17_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK46425.1"
                     /translation="EDKGVISPSDAVISMNLAEQTRKVLATLTPREEKVLRMRFGIGE
                     RSDHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLKSFVEN"
BASE COUNT         1752 a         3022 c         3622 g         2183 t
ORIGIN      
        1 tgtgccccct cgtcctcttg ctcacctacg ccctcggcct caccttgcag ggctagactc
       61 agcgcagcga tacactaggg cgtaacccaa tcctgatcgc gcggatacat tggtatcctt
      121 cgtcgcgcac agacatctgc aaagtcgcga tcattaaacg tcaccatggc gtcgattcgg
      181 cggcctgggt cggccaaaat cttgcggaga actgcgtcga ccaggctggt tcgacgtgat
      241 tgatttgcag tcgtgatcgc ttgttcgaga gcactttcac gataaggatc gtctggcact
      301 ctgtgcagtc ctggcgtttg taattctaga taaagtcgat tgagactgtc tgtgcttttg
      361 accaggcgcg ttgaaatgac ttcataaaga attggccacg gcagcagtgc gtggcatttc
      421 tctatgatgg gataaagaca gatggcctga ttgtgataag gatctcttgt atcaaacaaa
      481 gccaaccaga agccgctgtc gatcaatagc gtgtcagcga tactacccct catcgctttc
      541 ttctccgctg gcgctagggg catagccatc ccatggtgca ggtcgccgtc ccccgaacag
      601 aaaaagtccc ttcggtgcgt ccgtgccggg aagctttagg accagataaa tccgcgatct
      661 tttgctgatt tgtaccgctt gagacagtga tcccttgatc gctttgtcta gtgttccttc
      721 atattcataa tttgaaatgt aagccatgct ggaaccacat tctttggctc cgtggctgtc
      781 taaccaacga tacagcccat cataatcgcc attgatgcct agatcataag atagccaaaa
      841 ccgtgctttg ctcatcacct ggtcctttcc agtcagtggg cgtttacttt aggtcgatgt
      901 ccccgcgtgt caaggcacga tgccgcgaga aaaccaggtt gaacccaccc ctggcaatca
      961 ggtccaagcg cgctatttca cgtagacaat cgctttttcg atacatcaaa agtctgcttg
     1021 acgggatgac aagtcaccgc cgggacatcg caagtcaccg ccggaacatc gcaagtcacc
     1081 actgagacgt tgcacgtcag cgtcgaggcg ttgtaagtca acagcgagac atcgcgggtc
     1141 aacagcgaga catcgcgggt caacagcgag acatcgcaag tcaacagggg gacatggggc
     1201 gtctcttttt gacatccaga cttgggggcg aaaaaaaagt taccgacggc agcccttgcg
     1261 ggccgggtgc gcgtaggtag gcttttcggt ccgacgtgtg gatcgaaagg agatcctgcc
     1321 catggccaaa ccaagcacca aaaatgagtc tgttttcgac ctgcctctcg ccgagctgac
     1381 ccagcgggtc gaaaaagcga tgtcgctgct cgccgaagcg caggcgcttc tgcccggtct
     1441 gcggaccctg acggaagacg atcggcgtca ctctgggggc aagtttcgca ccggcgagag
     1501 cgaggtgtta caccgcgtgc tggatgcgat ccgcgcccag ccggcctatt ttgcgtccct
     1561 gtccgatgaa gacgatggca atgacccgac ccagcttgag gtcgatctga ttgccgacag
     1621 gctggcgcgg cgcgatctgc tgtcgcagct ccaggcgaag tttgccgccg tggagcagcc
     1681 gctttcggat tcggtgctgc atctgggcga acagacgcgc ggggtgctgt tggcggcgta
     1741 tcggattgcg cggacggtgg cgcgtaccga tcggaagctg cgcgaccaga ttgcgccggt
     1801 gatcgatttt tatggggcga tggtccgccg tccgaaagcg accgaagaca agccccagcc
     1861 ggtctagcaa tttgctaagg ttcgggtgtg catagctcgc ctagcagctc atcgatcgct
     1921 tatccgattg tggcgacgga ggagccgccc tggcttgcgc cttttgtggc gcggcttggg
     1981 gccgacaagg tttcgaccgc atcgcaagac ctgcacgcct accgtcgcga catgtgggcg
     2041 cgagagctgc ttgccgtggc ggctgcgcag cctgccgggg tgcttcctgc ggctgtggtc
     2101 tggcccagct cgacggatga actgcaagca gtcgtcgaac tggcgctggc tcaccatgtc
     2161 agtctgaccc cgtttggggc cggatcgggg gtgacgggcg gtgcgacgcc atcggcagga
     2221 agtctggtcg tcgactgcaa gcgcctgcgc tcgttttcgc tcgaccgcga tcggatgacg
     2281 gtgcgctgcg gcgcgggctg gaacggctgg cacattgaac aggcggtgaa ccgtcacggt
     2341 ctttcgattg gccacttccc gtcgtcgatc atgtgttcga ccgtgggtgg ctgggtcgtc
     2401 acccgtggcg ccggccaaat gtcgaccaag tacggcaaga tcgaagacat ggtcgcggcg
     2461 ctgcagctcg tcactgggcg cggcgaaaag ctggaactgg actgccggca agcggggcct
     2521 gactgggttc agcttgtcgt gggcagcgag gggcggctcg gctttgtcag cgaagtcgag
     2581 cttcagcttg tccccatgcc gcaaaagcgg ctgctgcgcg gatactggtt tcccgacgta
     2641 aggagcggct gcgcagcgat tcagcaggtg ctacagcttg ggcttaggcc gtcggtggtg
     2701 cggctgtacg acgaaatcga caccctgatt gcaggcaccg gccagcacaa gtcgagcctc
     2761 aaccagccgc tgtttgccaa tctgagcggt acggcaggtc gatttttggg tcagctttcg
     2821 gggctgcttg gcaagttcga gctgggacgg tcgggaaggc cgcttgacct gaacgatctg
     2881 ctggagcttt tgcggcctga tgcggagcgg gcgcggcacc ggctggagcg ctgggtgctt
     2941 cagacggcgc tgcaaaagac cgaaccgatt gggacgctct tggactctgt gcttgcgcgg
     3001 ctgagcgtcg gctgcctgct tatcatgggc tgcgaaggcg agccagcaac ggcgacgatg
     3061 gaagaacagc tggtccgcga cgaggtgatt cggctcggcg ggcgcgattt gggaccagag
     3121 ccgggcgaac actggctgcg gcaccgctat gacgtcagct tcaagtggcc gcgggtgttt
     3181 gcggcaggcg cgatggcgga cacgatggag tttgccgcga cgtgggaccg gctgccgtcg
     3241 ctgtatcaga aggtgcgggc ggcgattttg ccgcatgcgc tgatcatggc gcactttagc
     3301 catgcctatc ccgacggctg ctcgatttat tttacgttca tgacccgggc gagcgcgcgc
     3361 gggtcgggtc cacagtacgc ccaggcccgt gtgctcgacg atcagcgtcg ctacgatgag
     3421 atatggcagg ccgggattgc ggcggcgctg tcggcggggg cgacgatagg tcatcaccac
     3481 ggcgtcgggc gtctgcgggc gccgtttttg gtcgacgaac agggcgaggg actggcgggg
     3541 cttgtggcgc tcggcaagac gctggaccca agcgggctat gcaatgaggg ccggctgttt
     3601 tcgcgcgacg tgcagctttc gcccaaacct gtcgtgctgg cagcgccgcg ggcggtgtcg
     3661 gtcaacgcgc agtcgctttt ggtcacggcc cccgccgatc tgtctttgcg ggaactggaa
     3721 caaaagctgc ggcaaaaaga gctgacgctc ggggggcttc cgccgtgggc ctatgcgcgg
     3781 acgctggggg atgcgctgcg cagcccgctt ccgtcggaag cctgtctgtc gtcgggtcgg
     3841 ctgcatgacc gccgggttca gcttcttttg gcaaggcgcg atggggcagg gggctccctg
     3901 ctggcgattc cgccgcagcc cgcaccgcgg cgatcgacgg gaccggactt gggtcagctt
     3961 ttcgtcgatg gactgtgccc gaacacttgt cagattgtgg gcgcgacgct gcgggtcgaa
     4021 cgatttttgg cggcccggcc tgcgctgacg tgtggctttg atgactggaa cgaggcgctg
     4081 tctgtgctgt ggatggcgcg acgctggcac ggcggcactg ggcttcattc gatccagctc
     4141 ttttcgcggg cgctcatcga cagcgtgttg ccgcaggcgg acctgccacc gggcaagctg
     4201 ctgctgcttg tggaaggcgg cggtccgctg tcgctggcga gcgagtcgct gcgtgagctg
     4261 tcgcaccggc tggggatgaa agcgggcgcg cgtcgcgggc aggtgcttcg cgatctgtgg
     4321 tcgccgcaac tggatggtgc gggatcgccc tttttggcgc tgggccctct ccatgagcag
     4381 catgtgagcc cgcaggagct tgcagccctg ggtccgcttt tgctccagcc gaactcgcgg
     4441 tggcttgtcg ctggggtgca tcggcatggc gtggcgctgg caagtccaga cgcgctgccg
     4501 gtgtcggcac cgcggagctt tccgcttttg gaacaggtgg cggcggcgct cgacgaggcg
     4561 tatccaccct tgtcgcggcc aagaggagcg caggagtaag cccatgcctg atctgtctac
     4621 gtccctagag ctgtgctctt tgtgccccaa gctgtgcagc cacgtctgtc cggtctatcg
     4681 cgcatcggcc aaagaaaccc tgtcgcccca ggccaagatg tcgagccttt tgcggctgcg
     4741 gcagtcgggg gatgcggcgc agcctgccga cgccttgccg ctgtacgctt gcactgcgtg
     4801 tggggcgtgc gcgacggcgt gtctgcacaa gatcgaacct gcgcagcacc tcgtgcgggg
     4861 gcgggccctg gtcgaaaggc gcgagctggg acatccggcg ctttctgacc tgccggaacg
     4921 ggtgtttcat cgggcgcagg agacatcgcg ggcgctcctg cgggagcctg acctgctgtc
     4981 gcggctggcc aagccggggg agattgggct tttgccgtcg tgtctgccgc ggcgagctgc
     5041 cggtgggggc ccggtttcgg aagccaagca gcttttgtct gtgctcgatg cgctgcggtc
     5101 gcaccgggcg gactttccgc agtacggtct tttgacggtg ggcagcgctg gctatccgtt
     5161 gtatgcggcg gggctcgatg agtcgtttcg cctgtacgct gagtcgctgg cgccgaaagt
     5221 cgcagagctg tcggcgctgg tgacgacgtg ctcggcgtgt acgtatttgc tcaaagtggt
     5281 gtatccgcag catggggtgc cgcttcagcc caaggtgctg cacttgtcgg agctgcttgt
     5341 gccctacgtg acggcgctgc cgattcgcaa gcggctgccg aaagtgcgct accacgcgcc
     5401 gtgtcacctg gaaaggcggc ttcagattga tgcgccgcgg catctgttgg cccggctggc
     5461 cgacgtggtg gaagatgtca cgccggctgg ggaagacggg ctgtgctgcg gggcaggggg
     5521 acttttgccg cagaccgatc ctgcgcttgc gcgggcgatg gcgcacgagt cgatggcgca
     5581 cgtcgaggag ctggttccca cggtgagcgg ctgcccgacg tgcggctatc acctgtcgca
     5641 gagcggccat ccggtgcgcg acctgctgga tgtgctgtgc gacgccatcg gttagggccc
     5701 tgcggcgtcg aagcgggcgt gctgaacgac ccacgtcgtc ggtgaatttc ggagcgtgcg
     5761 gaagagggaa cctgggccac agaaagcgcg tactgtggcg acatgttctg ctgcacagat
     5821 ttgtttcttc ccaggtggct gccggcgctt ggcttgctgc tgctgacggc gtgccacggt
     5881 agcaagccga cggtgttccc aagtcccctt ccgacccagc caagcgcgac cgtgcgtgag
     5941 atcgccgagc gctttgatgt ggtgtgtccg cccggtccgc atggcgcgcg ggtcgcttct
     6001 gtgatttcgc cgcgctttgg tctgccggtc ttttcggttc cgtcggcaag cttcccgatc
     6061 gagctgcttg cgcgggggcc aaagcctgca cttgtcgctg cgttggctgc tccaagtctc
     6121 gatgaccaag cggcgcagcg ctgtctgtcc gccgcgcctg gaagtcagtg cgtgccgctt
     6181 gtgctggccg acgtcagcag cacagacatc gcaggcacgg gctttgcgac actccgcgcg
     6241 acggcgactg ctgcgggggt tggcgtgggg ggctgggatc tggtggtcca atcaggttcc
     6301 gatccgccgg agcggctcca ccgcagtgtc tggatcgagc caagcgatcc gtcgttgcct
     6361 gggccgctgc gcatcgtcca cctgtcggat ctgcatctgg gcaagcatcc ggggacgacg
     6421 gacgggcttt tggggtcgct caaagccgcg atccaaaggg tcaatgccga aaaaccggat
     6481 ctggtggtgc tgactgggga tgtggtcgaa gacggcctac aggaagagtg gcacaagcgg
     6541 gcgaagacgg agcttttggc gatcacggcg ccgcttgcga tcgtgctggg caaccacgac
     6601 tacgcccact ttccgaagat tcgctcgcct gacaaagcgc cagatgggta ctttgccttt
     6661 tcgcgtgagt tccactcgct gcgtcgctat gggtttgcct accacggctg ggacttcatc
     6721 ggtttcgatt ccggtccgtc cgtgtttgcg gttcgcgtgc tgacgcgcgg ggtggacaaa
     6781 gagacggttg gctggctggg tcagcgggtt gaagatgccg caaaccacgg gcgcaaaggt
     6841 gtggtcctgt ttagccatgc accgacccga accgcgccag ttcatgcacc ggacagcgag
     6901 gtcagtggct tgtgcggcca catgctggaa ggccggcagc agatcgaaca gatcatcgaa
     6961 acgggagccg aaaggggcct gcgcatggtt catctgagcg gtcacaccca ctggctggag
     7021 ctgcacgagc tgcgaccgca ggcgcgtggg ggggaagatc gctggcagaa gcgggacgac
     7081 ggcaacgtgt gtggcgtggt gccggggggc gcgctgcttt tgaactcgcc gtcggccacc
     7141 caagtgacct ttcacaccgt ggcgcgcggc aatcggagcg gctttggcgt gctcaagctc
     7201 gatggcgaca agccagtggt ggagacgctg ctgtttgacc gggccggcag tggtggcaag
     7261 tgcagcctgc cctagttggt gtgggagagg cgcaggtcag ctcggtgccc gagcggacag
     7321 agcagccgac gcttcatttt gattcggtgc ccgtggtata agggccgcat gtcactgagc
     7381 aagcacatgc gcgatctggt cagccaccgg gtcacgttcg actacgaaaa cggctgccac
     7441 atcactggtt acgtcgccac cgtgcgtcct gccgagggcg aggttctgac gattgtcctt
     7501 tcgcacgtcg aagtttcaaa cgacgctggc gatgtgatcg agcgccaccg cgagatggtg
     7561 gtggtgccgt ccgtcctggt gaactatcgc cagacggaag gtccgtcggc gcgtcagcgg
     7621 tagcggatgc acgcagcccc accttcgggc gaccttcggc tgcttctgtt ttcgcacgtc
     7681 gtctgtcggc ctgaatcgga gctggatctc gggcaggcgg cgcttttgat tgcggagccg
     7741 gagtatcccg atctcgacat tgcgcattac ctgggtcaga tcgacttgct ggcagcgcgg
     7801 ctgtctgcac agctttcgac cacagcgacg aacggagagc gggcgcagca gcttatcgag
     7861 ctgctgtgtg aagacggctt tcgctgcgat ccgggggact acgacgatcc gcaagcgagc
     7921 tttttgaact acgtgctcga cgagcggcat ggtctgccga tcttgctgtc ggtggtggcc
     7981 ctggaagtcg cgcggcgctg cgcggtgcct ttgtccgggg tggcgtttcc gggtcacttt
     8041 cttttgcggg gggaaagtga aaaaggggcg ccgccgctgc tgttcgatcc gacgaccggc
     8101 gaggcgctgg agcacgccga cctgcatgcg ctgatcgtgc gcgcgactgg caaacagcgt
     8161 cagattcaca aaagcgacat cgagccagcg ccaaggcacg ccatcttggc ccgcatgctc
     8221 ggcaacttgc gcaacatcta cttgcgacac agcgatgtgc cgcgattaca gcttgccgcc
     8281 gagcgcttgc gcctgttaga cacacatctt ccgacgtcac aaggtcggcc aagaccaagc
     8341 caatttcact gatgcgatct tttcttcctc tcgtcttcgc gctttcgctg cttgcctgtc
     8401 agaaagcgcc tcctgcgcct cgggttccgt ctgcacaaga agtttcggcg gaacaggtca
     8461 tcgccagcta tgctccgtcc cggctgcggg tctttccgac atcgagcggg gattatgtgc
     8521 tgtggggcag cgagcgtccg ggcctgcctg cggtgtttcg tcctctgggc aagaccgaca
     8581 agcgcggtcc cgcaggcatc atgtcgcgcg cgggagagct ttcgtttgtc ggcgagcgga
     8641 tgattgaagc gccgctgccg acctcgccgg ccaagacgtc ggcctcgctg ccgcttttgg
     8701 tgtatgaccg cgatgccaag acggcgcagg tgcagacggt gacgctgcct ggtccgtgta
     8761 cgatgcggga tcgggcgctt atcgcaagcg atggccagga gctgtttgtg ctggcccgct
     8821 gtgcgatcga agaccaagcg atggtgctgc ggctcgatgc ggcgcttttg gttcgggcgg
     8881 cgcggacgat cgacggggca ggggcggtcg agctcctgct tcaccacggc gattcggact
     8941 atcttttgca agcgcgtcag gtgctgcggg tgccgccgca gggactgccg gtgattggca
     9001 cagtgccgcc gccgggtggg gatgccgaaa cccgcgacct ggtgatggca ggggatctgc
     9061 tgctggtcgt cgatggcgcc gcaggtcgtg tgattggcat ggaccggctg tcgatgggct
     9121 ggaagctcga aaagcgcttt tacatagatg gggcagttca aaggctgcgg gcttgcgcat
     9181 cgccaaaccg gctgcaaatc gtcttggcgg aacgcaccga cgcggggcgg accgagctga
     9241 tagggcttgg cctgtcgctc cagatggggc gacgtgattc gggaatgccg cagcgacttt
     9301 tgctaggtgg cggaccgtcg acaagtgatc acgaacttct gccgattgcc agtggggacg
     9361 aggcgatgct tttgcgaacc cacgacggca acacgggtcc gcaggtcgcg ctaagctatc
     9421 tgcacatgta acgtccgtcg caatcgctgc gtgccggtcg tcgattgggc gataatggga
     9481 gacgcatgct gctgctcggt tttctgatct cgctgtttat cgcctacggg gcgcatcgtc
     9541 gtggctcact gacacaaagt ggggcgctgt gggcggcgat tgtggggacc gtggtgttcg
     9601 ggtttggcga tgggattgcg ggtgtggcgc ttgtcgggtt ctttctgtcg agtacgtggc
     9661 ttggcaagct gcgccgcggc gaaaccgctg agtcttccga tgccgacgca gtgattgaaa
     9721 aaggcgactg tcgcgacgga ctgcaagtgc tggccaacgg cggaccttcg gcggtgtttt
     9781 gtacgctcta tgcattgacc gggaagacga tgtatctggt cggtggtctg gcaagcttgg
     9841 cggcagccaa tgcggatacg tgggcgacgg aaattggtct gcgttcgcct tctttgccgc
     9901 gccacatttt gacgttagaa gaagtaccgg ctggtacttc cggcgcgatc accgcgacgg
     9961 gaacgctggg gatggtggca ggggcggtgt ttatcgggct tttgacttac ctggaaccgg
    10021 gacggggaag cgtgttgcgc acgctgcttg tcatctttgg cggcatcttc ggcggctgga
    10081 tggattcggt gctgggcgcg acggtgcaag aaaaaaggca gtgcgtaaag tgcggcgaag
    10141 cgaccgagca gtcgcgtcac atctgcggcg gcgcgacgct ggttaccgaa ggaattgcgg
    10201 ggatcgacaa cgataccgtc aatgcgctgg cgacggcgat aggtggggcg gcagcggcgg
    10261 tggcttggca cttcctggtc ggcaagtaac caagccagcc acggctagtt ttcgacgaag
    10321 ctcttgagcc gcttgctgcg gcttggatga cgcagcttgc gcagtgcctt ggcttcgatt
    10381 tggcggatgc gttcgcgcgt gacttcgaag tcctgaccga cttcttcgag cgtgtgatcg
    10441 gatctttcgc cgatgccgaa gcgcatccgg agcacctttt cttcacgcgg cgtcagcgtt
    10501 gccagcactt tgcgggtctg ctcggccagg ttcatcgaga tcacggcgtc cgacggagaa
    10561 atcacgccct tgtcctcga
//
LOCUS       DAORGN010000178        10546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2679_length_10546_cov_6.084358, whole genome shotgun sequence.
ACCESSION   DAORGN010000178 DAORGN010000000
VERSION     DAORGN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10546
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_10546_cov_6.084358"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKL17_16715"
     CDS             <1..469
                     /locus_tag="PKL17_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNK46426.1"
                     /translation="YIPANLLLSGFVVLMLASAIQMLRGSRKKTEPKSGAAWWLGMLV
                     GFPTGLVAGMLGAGGGFLVVPALTIFGGLAMEQAVGTSLLVIAMQAFAGSLGYLGHST
                     VDFRLVGMLASVMAVGSLGGAVLSQRVPAAFLRRLFAALLIAVAIQMLVQTFF"
     gene            541..972
                     /locus_tag="PKL17_16720"
     CDS             541..972
                     /locus_tag="PKL17_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNK46427.1"
                     /translation="MHNFTPGTAVIGGLLIGAAATLMLLLKGRIAGISGIIGGLLHPS
                     HGDSSWRAMFLLGMMAAPLGSLLWFGKLPEATVTASNRMLIIAGLLVGFGTRLGSGCT
                     SGHGVCGLARRSQRSLVSTLTFIATGAIAVYASRHLLGGMP"
     gene            969..1403
                     /locus_tag="PKL17_16725"
     CDS             969..1403
                     /locus_tag="PKL17_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNK46428.1"
                     /translation="MTTKRGVTSLLLGFLFGTGLLVSGMTNPQKVQDFLDFTGTWDPS
                     LSLVLGGALLVSLISFPLIVKREKPVLGQKFHLPTAKDIDAKLVIGAALFGTGWGLSG
                     FCPGPAMVSLSTGSTSAMIFVVAMLLGMALHRTYHDLRSPSQ"
     gene            1444..2901
                     /locus_tag="PKL17_16730"
     CDS             1444..2901
                     /locus_tag="PKL17_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46429.1"
                     /translation="MQHRFFAACLVLASSACTSEGLDAVVPHFDPPSATTLPAQNTPI
                     HIINLDNEATVCYSTDGSMPDFAGGSCAKTVDASRTISVPACGFNLIRIAWSSGSDEA
                     NYKVESDACKSSCDPVIPWPNQELARAYAVYTDEVKCMLNNCQNPSSTGNWSTKCDSG
                     QVSWNVSLNGLRAISTFTYDACAHSVTINVDEGGKMVPRKFNLIVTGKLVQDTDFDGN
                     GNEGGNVSVSGDFRGNIISRIVLENKQRKGGTVDAGCAADPLDGKICAPGSAAIAYNF
                     PDWSCRGGICPIAAAGSCKMPDSDGDGIPDDKDNCAKIANTDQSDVDRDGTGDACDSD
                     PAFVVLRFKVGGRCLTLGSKKHIESTSTCEETDLRQQWRMFPDGTAYGFRNLATGECL
                     SQTGVLAGPWTVVTAPCDGSDKQRWKLEPYNQGGTDAKFPLRLHNVAENFCIYTDLTG
                     LTYGTVLNCDLAGTESNRKVGIALGGAFDKPPFVP"
     gene            2988..3773
                     /locus_tag="PKL17_16735"
     CDS             2988..3773
                     /locus_tag="PKL17_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46430.1"
                     /translation="MRSFFPALFATCLLFPWSARSALAQDADKNKADEKPGARKSVNL
                     LINDPRLAPVDTTMSLGDWGAIRRAASTRFRPSREMITTDNKPKVLAQQLLSAQPVPP
                     EPQGRKLSTDDHIDAQDGVDAVRKSYERKPAQRSVEITVHTGPTGQIDSMAVTLPSGS
                     PRFDEEAMTCLRDAFSAYPPLEERRPYMSRWRVSAGYGISLPRTLAPMTPRTSNGRLP
                     ARGIPIPVPLWGTFDETKGTAKTNYAFGDQIESQVELLSHSPE"
     gene            4407..4769
                     /locus_tag="PKL17_16740"
     CDS             4407..4769
                     /locus_tag="PKL17_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46431.1"
                     /translation="MPLIRVYEESRNASVLSSGYNIDGSVTQLAKRVNPQGDLEVSAD
                     VSLIISVLPGRRVIGMVSGGATVIGSSTVDTHFMTLLVQNLQQEAITQAAHEAAASWV
                     ESLSRQQGRKNPKLVAMR"
     gene            complement(4766..5857)
                     /locus_tag="PKL17_16745"
     CDS             complement(4766..5857)
                     /locus_tag="PKL17_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNK46432.1"
                     /translation="MQYQSPSPTTRNESAPKPNQGREPFLHRMARRSLTVTLVFVLAT
                     LCTACLPLLLLVAGVVDVLSPRRTRFAFCRGLLFFVSLLAMEAIAICAVTLVWLLHLL
                     RRNPQTFERHNRSAQTLWSSTLYRIGERLFSLRTHIEGAIPPPHPDAAQVAPLLVFVR
                     HSSTADTVLPVLILANRGYRLRYVLKRELLFDPCLDIVGQRLRNCFVSRGGKDTDQDL
                     RGVLDLATDMTSGDALVIYPEGTRFTAQKRERTLARLRSHGPQDALTLAESLQHTLPP
                     LQRGPLQLLRENQRADLVLMAHCGLEPAGSLKELLAGDLIGEQVFIHFEHIPFAALPK
                     DPDAQRQFLSEKWRNIDAWIAAHKQRDGG"
     gene            5946..7280
                     /locus_tag="PKL17_16750"
     CDS             5946..7280
                     /locus_tag="PKL17_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNK46433.1"
                     /translation="MDEQVLIVGGGLTGLSTALHLPSHVRWSLFEKDARPGGHARTEC
                     RDGFYFDKTGHWLHLRDPGMISLVGELFPGGSPERELTKVARKARIFSSGVLTRYPFQ
                     ANLFGLPPQVISECLLGFIEALSDPRSAEPAKHFADFCDKRFGKGISRHFMLPYNRKL
                     WGVDPAEITAAWCSRFVPIPKLADVVKGAVGDTPPELGYNAHFLYPQTGGIETLVHRI
                     VERLPKDRLHRGAELEQLELRTKTAQIAGERIPYSSLVATLPLPELCRRIVDLPDTLT
                     PWVDKLRCTPVRYLNVATKVPPRSDYHWLYVPEDRYPFYRVGVYSNAVPSMAPSGCGS
                     FYVELSERGVIDVQALLPQVLDALVTAGILHSKEDLLFADLREIDYAYVVFDDNYYPA
                     LAALVPFLEANGIYPRGRYGSWTYNSMEDCLMLGRDVAATLAASKLSEQKKS"
     gene            7284..8033
                     /locus_tag="PKL17_16755"
     CDS             7284..8033
                     /locus_tag="PKL17_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK46434.1"
                     /translation="MATNTRPPNVSIVIPVYNEEGILHSAVVDLIERLQTAQKTTPIL
                     ADYEILLSENGSSDATVQIGQELAARYPQVRIDSLGRPNYGAAMKAGIERAAGTYVMC
                     DEIDLCDVDFYQRALALLEPGQADLVVGSKAMSGAHDKRPLLRRVATQTINGMLRVLL
                     DFRGTDTHGLKAFRRQRLLDTVSHCVVDKDLFASEFVIRAQREGKRVQEIPVHIVEKR
                     PPSINLFKRVPNVLKNLARLTYVIRIQSPHD"
     gene            8058..9623
                     /locus_tag="PKL17_16760"
     CDS             8058..9623
                     /locus_tag="PKL17_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HNK46435.1"
                     /translation="MKTLTSFLKGEWVSGQGHAAVLHNATTEEPVASASTHGLDMEGA
                     LRYAREVGGPALRKLTFTERGVMLKKLADAISAHRDELLLLGILNAGNTRSDAKFDVD
                     GASATFAFYAELCKSLGDARFFVDGEAIPLGRTSRLSGQHILTPRLGVAIHINAFNFP
                     AWGLAEKAATAIAAGMPFIAKPATSTALLAHRLFEVMVASGALPDGAGQIICGSVGDL
                     FDHLTGQDVVAFTGSADTAAKLRGHKRVLEKGVPFNAEADSLNIAVAGPDLESGSPTY
                     DLFISCVVKELTQKAGQKCTAIRRIFIPADKIDQVVPDLVDRIAHHKVGAPNLDDVTV
                     GPLSTAQQKTDALAGIRLLAQEARVVLGGADKIELHGTDPHKGYFVMPTLLVADDADS
                     LSHVHKHEVFGPVATVIPFRDTEHLFALAAQGEGSLVASVYSDDKTFQNDCVMGLSAY
                     HGRICLGSEKVAGSFIPPGTVMPHLIHGGPGRAGGGTELGGLRGMQFYMQRTAIQGFG
                     PYLESLAASGKKI"
     gene            complement(9620..>10546)
                     /locus_tag="PKL17_16765"
     CDS             complement(9620..>10546)
                     /locus_tag="PKL17_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNK46436.1"
                     /translation="LIVLKFDRLSRSIKHFCELYERFFKDGRKELVAIRESIKLDSSL
                     GRALVSILLVFAQMEREATGERTREAIRHLRSRGYHFGKVPYGMRAVTAPDNPRFRIL
                     AEEPEEQKILAQMKAWHEERVPISQMADRLNQLGAKPPQGEKWSKSLIYNLRIRNHWH
                     NPRPHNIRPHSDEEVRDRIVELHNGGHTPKQIAEKLNDEEFVPLKGRSFTERSVRSLL
                     SRTEEAKHMTPKKFLEAMLDRLEREHEKHFPDEPWERPGYPKLARFLMQAGYVTPRGH
                     THWWPAQVQQLLDGRFDQYYIRSKHPVSIEQD"
BASE COUNT         2082 a         2937 c         3268 g         2259 t
ORIGIN      
        1 ctacatcccg gcgaatctgc ttttgtccgg ttttgtcgtg ctgatgctcg cgagtgcgat
       61 tcagatgctg cgcggcagtc gaaaaaagac ggagccgaag tcgggggcgg cgtggtggct
      121 ggggatgctg gtgggttttc cgacgggact tgtggccggg atgctcgggg cggggggcgg
      181 ttttctggtc gtgccggcgc tgacgatctt tggcgggctg gcgatggagc aggccgtggg
      241 gacgtcgctt ttggtgattg cgatgcaggc cttcgcgggg tcgcttggat acctggggca
      301 ctcgacggtg gatttccgcc tggtgggaat gttggcttcc gtgatggcgg tgggcagcct
      361 gggcggagcc gtcctgtccc agcgcgtccc ggcggccttt ttgcgacgac tgtttgcggc
      421 gctgctgatt gcggtggcga tccagatgtt ggtacagacg tttttctagg ctacggatcg
      481 gaagaacccg gttttcggac ggaatgaacc gggcttggaa ccagcagaga ggatgggctt
      541 atgcacaact tcacacccgg aacagcagtc ataggtggcc ttttgatagg tgctgcggcg
      601 acgctgatgc tgctactgaa aggtcgcatc gccgggatca gcggaatcat cggcggtcta
      661 ctacatccgt cgcacggcga ttcgtcgtgg cgggcgatgt ttctgctcgg gatgatggcg
      721 gccccgctcg gttctttgct ttggtttggc aaactgcccg aagcgaccgt cactgcgtca
      781 aaccggatgc tgatcatcgc tgggctcttg gtcggttttg gcacccgact gggaagcggt
      841 tgtacgagcg gacacggcgt ctgtgggctg gcgcgtcggt cgcaaagatc gctggtttcg
      901 acgctgacgt tcatcgcaac cggcgccatc gcggtgtacg caagccgcca tcttctggga
      961 ggcatgccat gacgacaaaa cgtggagtga cgtcgcttct tctcggtttt ttgtttggca
     1021 ccgggctgct tgtgtcgggg atgaccaatc cgcagaaagt gcaggacttc ctggacttca
     1081 ccgggacgtg ggacccgagt ctgagcctgg tgttgggtgg ggcgctgctc gtgtctttga
     1141 tctcgtttcc gctgattgtg aagcgggaaa agccggtgct tgggcagaag ttccatttgc
     1201 cgacggcaaa agacattgat gccaagcttg tcatcggcgc agcgctgttt ggaaccggct
     1261 ggggcctgag cggtttctgt ccgggtccgg cgatggtcag tctgtccacc ggttcgacca
     1321 gtgcgatgat ctttgtggtg gcgatgctcc tcgggatggc gctgcaccgc acctatcacg
     1381 atctgcgcag tccttcccaa tagcgcttcc cacaacgtcg cggcttggcg atatgctccg
     1441 cgcatgcagc accgcttttt tgccgcttgt cttgtccttg cttcgtccgc ttgcacaagc
     1501 gaaggtctcg acgctgtcgt cccgcacttt gatccaccga gcgcgacgac attgccggcg
     1561 caaaacacgc ccattcacat catcaacctc gataacgaag cgaccgtttg ctattcgacc
     1621 gatggatcga tgcccgactt tgccggcggt agctgcgcca agaccgttga cgcaagccgc
     1681 accatttctg tccccgcctg cggtttcaac ttgattcgga ttgcctggtc aagcggcagc
     1741 gacgaagcca actacaaagt cgaaagcgat gcctgcaagt cgagctgcga cccggtgatt
     1801 ccgtggccaa accaagagct ggcgcgcgcc tacgcggtct ataccgacga agtcaaatgc
     1861 atgctcaaca actgccagaa tccgtcgagc accggcaact ggtcgaccaa gtgtgattcg
     1921 ggccaagtga gctggaacgt gagcctcaac ggtctgcgcg ccatcagcac gttcacgtat
     1981 gacgcatgcg cccactccgt caccatcaac gtcgacgaag gtggcaagat ggtgcctcgc
     2041 aagttcaacc tgatcgtcac cggcaagctg gtccaagaca ccgatttcga cggcaacggc
     2101 aacgaaggcg gcaacgtctc cgtcagcggc gatttccgcg gcaacatcat ttcgcgcatt
     2161 gtgctggaaa acaaacagcg caaaggtggc accgtcgatg cgggctgtgc agccgatccg
     2221 ctcgacggca aaatctgtgc gcccggcagc gccgcgattg cctacaactt tcctgactgg
     2281 agctgtcgcg gtggaatctg tccgatcgcc gccgcgggaa gctgcaaaat gccggacagc
     2341 gacggcgatg gcattcccga tgacaaagac aactgcgcga aaatcgccaa caccgatcag
     2401 tccgacgtcg accgtgatgg cacaggcgat gcgtgcgaca gcgatccggc gtttgtcgtg
     2461 ctgcgcttca aggtcggcgg tcgctgcctg acgctgggaa gcaaaaaaca catcgaatcg
     2521 accagcacct gcgaagaaac cgatctgcgt cagcagtggc ggatgtttcc cgacggaacg
     2581 gcttacggat ttcgcaacct ggcaactggc gagtgtttaa gccaaaccgg tgtgctggcg
     2641 ggtccatgga cggtggtgac agcgccctgc gatggcagcg acaagcagcg ctggaagctg
     2701 gagccgtata accaaggtgg caccgacgcc aagttcccac tgcgtctgca caatgtcgcc
     2761 gaaaacttct gcatctacac cgacctgacc ggactgacgt atggcaccgt cctaaactgc
     2821 gacttggccg gcacggaatc gaaccgcaag gtcgggattg cgctcggcgg tgcattcgat
     2881 aagccaccgt ttgtgccgta attccgcggt cgttttcatt ccctacctgc acacggtttt
     2941 cccgaccgcg cacatgggcc gctgcgtttc tgggctaggc tgtgcagatg cggtcctttt
     3001 ttcctgcgct ctttgcgact tgtcttttgt ttccatggtc ggcccgatcg gcgctggccc
     3061 aggatgcgga caaaaacaaa gcagacgaaa agcccggtgc gcggaagtcg gtcaacctgt
     3121 tgataaacga tcctcgtctg gcgccggtgg acacgacgat gtcgcttggc gactgggggg
     3181 cgattcggcg ggcagcgagc acgcgctttc gaccctcacg cgagatgatc acgacggaca
     3241 acaagcccaa agtgctggcg cagcagctgc tttcggccca gccggtgccg cccgaaccgc
     3301 aaggtcgcaa gctcagcacc gacgatcaca tcgatgccca agacggtgtc gatgcggtgc
     3361 gcaagtctta cgaacgcaaa cccgcgcagc gcagcgtcga aatcaccgtt cacaccggcc
     3421 cgacgggaca gatcgattca atggcggtaa cgctgccgtc cggaagtccg cgttttgatg
     3481 aagaagccat gacgtgtctg cgcgatgcgt tttcggcgta tccaccgctc gaagaacgtc
     3541 gtccctacat gagccgctgg cgggtgagtg cggggtacgg aatttcgctg cccaggacgc
     3601 tggcgccgat gacgccacgg acttccaacg gacggctgcc ggcgaggggg attcccattc
     3661 cggttccttt gtggggcacg ttcgacgaaa ccaagggcac cgcgaaaacc aactacgcat
     3721 ttggcgatca gatcgaaagt caagtggaac tcctttcgca cagcccggag tgaaaaacga
     3781 cgtgcccaag ggatgcgcaa agcgatcgga ttgccatgct ggcgcggaat cttggttcta
     3841 ggtttttgga tttctcgacg ggaaattgaa cagttaggaa tctgtaccaa acggaacagg
     3901 gcgtttttgt gtgtgcggcg gcggcagatt ccgtcggagt ggtttttaca ggaattgcaa
     3961 aaattgtaga ttttgttgga tcgcaaaagg actcctttga tagattggga atgggccagc
     4021 ggtggggatc ttctcgacgg caagtgcagc gaggtgcgat tcccaagccg gctccatccg
     4081 cagcgcagtc tgatgactgc aaggggggtc gcatgcgata cgatcttgcg gctgcttcgc
     4141 gtccaaatca tcgtcgtttc ggaatgtttg gcctaaaaca gtcgattggc gacggggtag
     4201 ggcgtttggg attctgtctc tgtggacttc tgtctgtgtt tctgtgctct tcttttggcc
     4261 cgttgtctgc ggtttcggtc gcgcacgcgg aagaatccag aaaaatcgct acggtgattg
     4321 ggagctgtgg atcaaaagcc aagcctctcg tcggtgcgga tctgtcttcc gtgttgcgga
     4381 gtgcgcttgt aaaagaactc cgcagcatgc ctcttattcg ggtgtatgag gaatcgcgca
     4441 acgccagcgt cctgtcgtcg ggttacaaca tcgacggcag cgtcacgcag cttgcaaagc
     4501 gagtgaatcc gcagggggat ctggaagtca gcgcggacgt gtcgctgatc atttcggtgt
     4561 tgcccggacg gcgcgtcatc ggaatggtgt caggcggtgc gacggtgatc ggatcatcga
     4621 cggtggacac ccattttatg acactgcttg tgcagaactt gcagcaggaa gcgattacgc
     4681 aagcagcgca cgaagcggcg gcaagctggg tggaatcgct gtcgcgtcag caggggcgga
     4741 aaaatcccaa gctggtggcg atgcgctagc ctccgtcgcg ctgtttgtgg gcggcgatcc
     4801 acgcatcgat gttgcgccat ttttcagaca aaaactggcg ctgcgcatcg ggatctttgg
     4861 gcagcgcggc gaagggaatg tgttcaaagt gaatgaacac ctgctctccg atcaaatcac
     4921 cggcgagcag ttctttgagt gaacctgcag gttccagtcc acagtgggcc atcagcacaa
     4981 gatcggcgcg ctggttttcg cgcagtagct gcaatggacc gcgctggagc ggcggcaggg
     5041 tatgttgcag actttctgcg agcgtcagcg catcttgtgg tccgtggctg cgcagccgtg
     5101 ccagtgtccg ttcccgtttt tgcgccgtga aacgggttcc ttccggataa atcaccagcg
     5161 catcgccgct cgtcatgtcc gtcgccaaat cgaggacacc gcgcagatcc tgatcggtgt
     5221 ctttgccccc gcgcgaaaca aaacagtttc gcagtctctg tcccacgata tctagacacg
     5281 gatcgaacag cagctctcgc ttgagcacgt agcgcagacg gtagccgcgg ttggccaaga
     5341 tgagcaccgg caacaccgtg tcggcagtgc tgctgtgacg aacaaacacc agcaaaggcg
     5401 caacctgggc agcgtcggga tggggcggcg gaatcgcacc ttcgatgtgc gtgcgcaggg
     5461 aaaacagccg ctcgccaatt cggtacagcg tcgatgacca aagggtctgg gcgctgcggt
     5521 tgtgccgctc gaaggtctgt ggattgcgac gcagcagatg aagcagccac accagcgtca
     5581 ctgcacaaat ggcgatcgct tccatggcga gcagcgaaac gaaaaacagc agaccgcggc
     5641 aaaaggcaaa gcgtgtccgc cgcggcgata gcacatcgac aaccccggca accagcaaca
     5701 gaagcggcaa gcaggccgtg cacagcgtcg caagcacaaa caccagcgtg acggtcagcg
     5761 atcgccgggc catgcgatgc aaaaacggct ctcggccctg gtttggcttg ggtgccgatt
     5821 cgttccgcgt ggtgggcgac gggctttggt actgcatatt cccttttgat gtacgacgct
     5881 gccgaaagtg cgcgaaaaac tgccgttgcc gagaggggca aaggcatgat agtagcccaa
     5941 aggacatgga cgagcaggtg ctgattgttg gcggtgggct aacgggctta tcgacagccc
     6001 tgcacttgcc gtcgcacgtc cgctggtcgc tgtttgaaaa agacgcgcga cccggcggtc
     6061 atgctcggac cgagtgccgc gacggttttt acttcgacaa gaccgggcac tggctgcatc
     6121 tgcgtgatcc ggggatgatt tctctcgtcg gtgagctgtt tcctggcggt tcgccggaac
     6181 gggaacttac caaggtcgcg cgcaaagccc ggattttttc gagcggcgtg ctgacgcgct
     6241 atccatttca agccaacctg ttcggtctgc cgccgcaggt gataagcgag tgtctgctcg
     6301 gcttcatcga agccttgtct gatcctcgca gcgcagagcc ggcgaaacac tttgccgatt
     6361 tttgtgacaa gcggtttggc aaaggaatct cacgtcactt catgctgccg tataaccgca
     6421 agctgtgggg cgtcgatcct gcggaaatta ctgcggcatg gtgttcgcgt tttgtgccga
     6481 ttcccaaact ggccgatgtg gtaaagggag ccgtcggtga cacgcctccc gagcttggtt
     6541 ataacgcgca ctttctgtat ccgcagacag gtgggatcga aacgctggtc catcgcattg
     6601 tggaacgact cccaaaagac agactgcatc gcggggccga gctggaacag cttgagctgc
     6661 gaacgaaaac ggcgcagata gcgggcgagc ggattccgta ttcatcgctt gtggcgacgc
     6721 tgccgctgcc ggagctgtgc cgccgaattg tcgatcttcc ggacacgctt accccgtggg
     6781 tcgacaagct gcgctgcacg ccggttcgct atctgaatgt cgcaaccaaa gtcccgccgc
     6841 gatcggacta tcactggctg tacgttcccg aggatcgcta tccgttttat cgggtcggtg
     6901 tgtattcgaa cgcggtgccg agcatggcgc cttctggctg cggttccttt tacgtcgagc
     6961 tgtcggagcg cggtgtgatc gacgtccagg cgcttttgcc gcaggttctg gatgcgctgg
     7021 tcacggcggg aatcctgcac agcaaagaag acctgctgtt tgccgacctg cgtgaaattg
     7081 actacgccta cgtggtcttc gacgacaatt actatccggc gcttgcggcg ctggttccgt
     7141 ttttggaagc aaacggaatc tatccgcgtg ggcgatacgg atcttggact tacaactcga
     7201 tggaagactg cctgatgctg ggacgtgacg ttgcggcgac gctggcggct tcgaagttgt
     7261 ccgaacagaa aaagagttaa gccatggcga caaacacaag gccgccgaat gtctcgattg
     7321 tgattccggt gtacaacgaa gaaggaatcc tgcactcggc ggtggtcgat ctgatcgagc
     7381 gcctacagac agcgcaaaag acgaccccga ttttggcgga ttacgaaatt ctgctgtcgg
     7441 aaaatggatc gtcggatgcg acggtgcaga tcgggcagga actggcggcg cgctatccgc
     7501 aagttcgcat cgattcgctg gggcggccaa actacggtgc ggccatgaaa gcaggaatcg
     7561 agcgcgcggc tggcacgtat gtgatgtgcg atgagatcga tctctgtgat gtcgattttt
     7621 atcagcgcgc gcttgcactt ttggaacccg gacaagcgga cttggtcgtc ggttccaaag
     7681 cgatgagcgg agcccatgac aagcgtccgc ttttgcgccg ggttgccacg cagaccatca
     7741 acggaatgct gcgcgtactg ctggattttc gcggcaccga tacgcacgga ctcaaggcgt
     7801 tccgcaggca gcggctgctc gatacggtgt cacactgcgt ggtggacaag gatctgtttg
     7861 catccgagtt tgtgattcgc gcgcagcgag aaggcaaacg ggtgcaggaa attccggttc
     7921 atatcgtcga gaaacggcct ccctcgatca atttgtttaa gcgggttccc aacgtgttaa
     7981 agaacctggc gcggctgacg tatgtgattc gcatccagag tccgcacgac taaaaacgac
     8041 gaagaaaaag tacacagatg aagacgctga caagctttct caaaggcgag tgggtttccg
     8101 gacagggtca tgccgccgtg ctccacaacg ccacgaccga agaaccggtg gcatcggcca
     8161 gcacccacgg actcgacatg gaaggggcac tgcgatacgc ccgcgaagtc ggtggtccgg
     8221 cgctgcgcaa gctgacgttt accgagcgtg gtgtgatgtt gaaaaagctc gcggatgcga
     8281 tctctgcgca ccgtgatgag ctgctgctgc tcggaatcct aaacgccggc aacacccgat
     8341 cggatgcgaa gttcgatgtc gatggtgcgt cggcgacgtt tgcgttttat gcagagctgt
     8401 gtaagtcgct cggggatgcg cgcttttttg tcgatgggga agcgattccg ctgggccgta
     8461 cttcgcgtct ttcgggtcag cacatcctga ctccgcgcct cggtgtggcg attcacatca
     8521 atgcgttcaa ctttccggcg tggggacttg cagaaaaagc tgcgacggcg attgcggcag
     8581 gaatgccgtt cattgccaag ccggcgacat cgaccgcact gctggcgcat cggctgtttg
     8641 aagtgatggt cgcaagtggc gcccttccgg atggcgctgg gcagatcatc tgtggcagcg
     8701 tcggtgatct gtttgatcac ctgaccggac aggacgtggt cgcgtttacc ggatcggcag
     8761 acacggcggc gaagctgcgt ggccacaaac gggttctgga aaaaggcgtt cccttcaatg
     8821 cggaagcgga cagcctgaac atcgcggtgg ccggtcctga tctggaatcc ggaagtccca
     8881 cctacgatct gttcatcagc tgcgtggtca aggaactgac gcagaaagcc ggccagaagt
     8941 gcaccgccat ccgtcggatt ttcatcccgg ctgacaagat cgatcaggtg gttccggatc
     9001 tggtcgatcg aattgcacat cacaaagtcg gggctcccaa cctggatgat gtgacggtgg
     9061 gcccgctgtc cactgcacag cagaaaaccg atgcactggc tggcattcgg cttctggccc
     9121 aagaagcccg cgtcgtgctg ggtggggccg acaagatcga actacacgga accgatccac
     9181 acaaaggcta ctttgtgatg ccgacgctgc ttgttgcgga cgatgcggac tcgctttcgc
     9241 atgttcacaa gcatgaagtg tttggtccgg tcgcgaccgt gattcctttc cgcgataccg
     9301 aacatctgtt tgcgctggct gcacaggggg aaggaagcct ggttgcaagt gtatacagcg
     9361 acgacaaaac cttccaaaat gactgcgtga tgggactgtc tgcctatcac ggacgcatct
     9421 gtctgggcag cgaaaaagtc gcgggctcct tcattcctcc gggcaccgtg atgccgcacc
     9481 ttatccacgg cggacctgga cgggcagggg gcggcaccga gcttggcggc ctgcgcggca
     9541 tgcagtttta catgcagcga acagccattc agggattcgg tccgtacctt gaatcccttg
     9601 cggcatcggg aaagaagatt tagtcctgtt cgatggacac cggatgcttt gatcggatgt
     9661 agtactgatc aaatcgtccg tcgagaagct gctgtacctg cgcgggccac cagtgcgtat
     9721 gtccgcgcgg cgtgacgtat cctgcctgca tcaaaaatcg cgccagtttc ggatatccgg
     9781 gtcgctccca cggctcatca ggaaagtgtt tttcgtgctc tcgctcgagt cgatcgagca
     9841 tcgcttcgag aaactttttc ggcgtcatgt gcttcgcttc ttctgtgcgt gacagcagcg
     9901 agcgcacact ccgttcggta aagctgcgac ctttcagggg gacaaactct tcgtcgttta
     9961 gcttctctgc aatctgtttg ggagtgtgtc ccccgttgtg caattccacg atccggtcac
    10021 ggacttcttc gtcgctgtgc gggcggatgt tgtgcggcct tgggttgtgc cagtggttgc
    10081 gaatccgcag gttgtagata agactcttgc tccacttttc cccctgcggt ggcttggctc
    10141 ccagctgatt gagtcgatct gccatctgcg agatcggcac tctctcttca tgccacgctt
    10201 tcatctgcgc caggattttc tgttcttcgg gttcctcggc gagtattcgg aagcgcggat
    10261 tgtccggagc tgtcaccgcg cgcattccgt agggcacctt cccaaagtga tacccacgac
    10321 tccgcaagtg ccggatggct tcgcgtgttc gctctccggt cgcttcccgt tccatctgcg
    10381 cgaacaccag cagaatgctc accagcgcgc gtcccagcga cgaatcgagc ttgattgact
    10441 cgcggatcgc caccagttcc tttcgtccat ctttgaagaa gcgctcgtac agctcgcaga
    10501 agtgtttgat cgaccgactg agcctatcga acttgagcac gatcag
//
LOCUS       DAORGN010000179        10426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2730_length_10426_cov_5.139524, whole genome shotgun sequence.
ACCESSION   DAORGN010000179 DAORGN010000000
VERSION     DAORGN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_10426_cov_5.139524"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /locus_tag="PKL17_16770"
     CDS             complement(<1..2013)
                     /locus_tag="PKL17_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein kinase/ABC
                     transporter substrate-binding protein"
                     /protein_id="HNK46437.1"
                     /translation="MSGPNQKVPPGGPGSRQTPGNRLGGPGTGSVSDEKISDTIDIPV
                     GMAVPTQSEPSLSRRNDARLGQMFGKYRVEAVIGKGGMGLVYEGEDTVLSRRVAIKFL
                     PESLTSSPKAVERFITEAQVAGRLNHPNIIAIYDIGQEDEHYYIVMELLNPSSASSHV
                     KEKGPLHYAEACKIIMDCCSALHAAHQAGLVHRDIKPDNILCSPMGTTKLVDFGLVKD
                     VHFDEGALTQTGVVAGTPLYMSPEQASDGEVDRRSDIYSLGATFYTLLTAKPPYTGDA
                     APQIMFKHVTAPTPDPRDISPDVPESVVAILRRAMEKSPADRYQSADEMRQALETVLA
                     TMTRKPFVFLVEHEPSLLMMKRPLRVTLPPPLNPRLSAQMPIVGQPSNRSLVPLENSN
                     PRSSGQSSRISGNSTRVSGQNAPIGDMPSVPSGRLSQQQSSYPAFLGGIGVAAGLLAL
                     VLVLWRVFKPVEPLVNVPSQKDAGSVAALAPLPPIRVGVLNSLSGTMAISTRPIVEAT
                     LLAIDEINDQGGVLGRRIDAVRVDGRSDNATFALETERLIKQEHVTTIFGGWTPSNRR
                     AMKPLVEKYDHLLIFPARDEGMEDSQNIIYLGSTPNQQVIPATHWALEKLGARRLFFI
                     GLDGLWGHISHELVRDVIKGTQAKIVGKEFVLLSETDFSSIVKKIQA"
     gene            2118..4019
                     /locus_tag="PKL17_16775"
     CDS             2118..4019
                     /locus_tag="PKL17_16775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNK46438.1"
                     /translation="MRVHRSLLPVIAGLPLGLAMAQVPPPGVPPAAATQAPPAAGTVQ
                     AAPAVPGQPGDPSAAAKKPRKFWIDGKYRELKKGEVRPYTPPNLPPEPDPQPSAARVF
                     EVAKPEEGDPQVLARPYYGSPMVGTVVPGTRIAVRGEVIAKSSRWCHSKRWFAVMPFG
                     WMCSDHGKATDAPATTDPVYKIVPGERVPYRYVMVSAQEMPMWTSLDAFKNNEEPERK
                     LAKGDSVAIEKNFKHEGETYLQSVEGKILPAKGTYQMPATSQWHGVLIDDKTHLPFGW
                     VLSEQLKAQAAPDQPTVTGMLKRRVQVDILEEMTVKGKRWLRVKPSAAPTKEFPLATE
                     PLPPPPIPVAAPGTIATAAKPATPTPAAPVVTPTSPAVAVAPATPAPAGTAATPPAPP
                     VEQAMWVPAWAVNEVRQIAKPAGIGVNAQWFDADLSEQVLVAYENDKPVYATLVSSGK
                     NNATPLGIYPVWARVAAITMKSQPYDDKPYFVNMVPWSTFFQAHNAIHGAYWHDRFGY
                     VKSHGCINVAPLDARYVFELLKPAVPPGWTSVRPTDVTQWPMLYVHDSGRRPAFHQDR
                     PIGAPDREDESERFEETNRLRIEQGLPPLPPPGTKPPAAATPGVPAAAAPVPAAAPAP
                     VANPALAPR"
     gene            4120..5034
                     /locus_tag="PKL17_16780"
     CDS             4120..5034
                     /locus_tag="PKL17_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46439.1"
                     /translation="MIKVRAFPWLLSLAFSTVSLTGCPEQAEQPQATGPKPDTVCPKE
                     LGASCKVLKDEFDEKKLVAEYHVLVSPDTKHDDADNYLKALYRHMMTRRDVAPTQLGG
                     FVYTNEAQFTTPPLSPVGSVTQKPGEKSPTFENKIALELWQQAEQAIKLSARADRKLK
                     RKLEYVAEPTKSMVTVILPFTEGATEEWAKEIDYSQVIAYFSELSQQLFNNIPDLKSF
                     LYRARWKDQDVAQIEITRADYEKINLADVEHRVGQLAGRTFLELTTGKGGSEASVEKA
                     HNRRRAAEYKKALDQIKGGKVIINPLLK"
     gene            complement(5068..5955)
                     /locus_tag="PKL17_16785"
     CDS             complement(5068..5955)
                     /locus_tag="PKL17_16785"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46440.1"
                     /translation="MRIAHISDLHALDLSGASPLRFLSKRFFGWLNLLRKRRAAHPSF
                     LLDALCDDLRQQPLDHVVVTGDLTNLSLPSEFQRARQALDSLPQGPQGVTAIPGNHDV
                     YVWEAYFGHTFEKMLLPYAQSDGATEGELPTWPIVRKRDGVAIVGCSTALPSPVPLAD
                     GWLGGKQLQRLEQMLDGLRDHFRVLLIHHPPLPQHLDVLRALRDRGRLHDVLRRVGCE
                     LVLHGHEHRDLRATLDGPNGSIPVIGVGSGTYNHPLPERRARYNVYTIDGQGASAKLT
                     VEQRVHDPQSGRFVPSSPV"
     gene            6015..7448
                     /locus_tag="PKL17_16790"
     CDS             6015..7448
                     /locus_tag="PKL17_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNK46441.1"
                     /translation="MTHSQVRKLPVGFVDTLLQIVGPSGLITDAEAIEPFLTEPRGTY
                     RGQTDVVVRPASTEELASVVAACANARVPMVPQGGGTGLCGGAVPAPSGEQIVVSLAR
                     MNRIRALDASSFTMTVEAGCILADIQRAAEEAGLLFPLSLGAEGSCQIGGNLSTNAGG
                     TQVLRYGNSRDLVLGLEVVLPSGEILDMLRGLRKDNTGYHLSGLFCGAEGTLGIVSAA
                     VLKLFPKPREVVTALLAVPSLDAVIKLLSLARTATGDQLSGCEFIPRIALDMVLRHIP
                     NTRDPFPTTYPHYLLIEAQSGSEDGRLGREVEKLLADAADADLILDATVAQNPSQADA
                     LWHIRESIPDAQKPEGLSVKHDVSIPIHLVPKFVAEATRLVEAEVPGIRVVAFGHVGD
                     GNVHFNLSQPVGMDRATYAQHREQLSHRVHDLAMSLGGSFSAEHGIGQLKRQELVRYR
                     SAVELSVMRKIKAALDPLGLMNPGKVL"
     gene            7566..>10426
                     /locus_tag="PKL17_16795"
     CDS             7566..>10426
                     /locus_tag="PKL17_16795"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNK46442.1"
                     /translation="MKLSQLLREVALRPNAATAFSTETDLDAIEIGDVREDSRQVQPG
                     DLFVAVPGQTVDGHHFLSDAAQRGAKAAIVEQIRPDCKLIQIPVENATEALAQLAMNR
                     YGRPSDSLTLVGITGTNGKTTSNFLVETLLEEADLRPGLLGTVLYRGPGVSQPAPFTT
                     PTALLLHQTLAVMAEHGAKAVTMEVSSHALALGRLFGVRFRVAAFTNLTQDHLDLHGT
                     MDEYFAAKCQLFHRHLLPAERGGRAVINVDDPWGRKLFDSLPPEQRIGFSVDGPADIS
                     IASERTIVEGISAVFRTPLGDVTIRSPLTGRFNLSNLALSVGIGVALGLSAVQIGRGL
                     SRLSGVPGRLERVPSRRGPTVFVDYAHTPDALSRVLQTLRPLTRSGTKPSRLLVVFGC
                     GGDRDAGKRPLMGQAAAEEADLVIVTSDNPRTEQPQRILDMIVEGVEKTGRLPLISSR
                     SDLASATSGYLVEIDRRQAIVAAILAAQPDDVVLLAGKGHEDYQVIGTSKQHFDDREE
                     AQKALALRPETQPRSGTPTPPVMPKLQPSIELPLERILSATAGRLVRGSTPRFAAVTI
                     DSRSVTPGCLFVAIRGDRLDGHTFIAQALQNGCSGVLVEKDKLHLVPDQPGLTVVEVA
                     DTIVAIGQIARAHREAPEIAAKLKVIGVTGSSGKTSTKDLIAAIFAAHVQDSSDFIKT
                     DGNLNNHLGVPLTLLRLRPGQRYAIVEMGMSARGEIAYLTSLCRPDVGVITNVGPAHL
                     ETLGTIENIAAAKGELFAGLSDGATAVFLDGHALVREQAMRAGALSTRLRLGLASCGT
                     TAATHASVHVRLRREDLDGLHLEISFPQLPGSPSQTLHVPLIGAHQADNAALAAAAAL
                     ALDVPPATIAAGLARVTPGKHRGQLLTIGGRRVLDDCYNANPSSVSAALRSLSGLRQG
                     GQAIAVLGDMLELGPTEAELHAKVGRLAATLGIGKVITVG"
BASE COUNT         2102 a         3275 c         3136 g         1913 t
ORIGIN      
        1 agcctggatc ttcttcacga tcgacgaaaa gtccgtctcc gacagaagca caaactcttt
       61 gccgacgatc ttggcttggg tgcccttaat gacatcgcgc accagttcat gcgaaatgtg
      121 gccccacagc ccgtcgagac cgatgaaaaa cagccgccgc gcgcccagct tttctagcgc
      181 ccagtgcgtc gctggaatca cctgctggtt gggcgtcgat cccaaataaa tgatgttctg
      241 cgagtcttcc atgccctcgt cgcgggccgg gaaaatcaac aagtgatcat acttttcgac
      301 aagcggcttc atcgcgcgcc ggttcgatgg cgtccagccg ccaaagatcg tcgtcacatg
      361 ctcctgtttg atgagccgct cggtttcgag cgcaaacgtc gcattgtcgg aacgaccgtc
      421 gacacgaact gcgtcaatcc gtcgcccgag cacgccgcct tgatcgttga tttcatcgat
      481 tgcgagcagc gtggcttcga cgatcggtcg agtcgaaatc gccatggtgc ccgacaagga
      541 attgagcact ccgacgcgaa tcggtggaag aggtgccaaa gcagcaacgc tgcccgcatc
      601 tttttgtgac ggcacgttca ccagcggttc caccggctta aacacccgcc acaacaccaa
      661 taccagtgcc aaaagccccg ccgcgacgcc aatccccccg aggaatgccg gatagctgct
      721 ctgctgctgc gacagccttc ccgacggcac acttggcatg tcgccaatcg gtgcattttg
      781 accggacacc cgcgtgctgt ttcccgaaat gcgcgagctt tgtcccgagc tgcgcggatt
      841 tgagttctcg agcggaacca gcgaccgatt gctcggctgg ccaacgatcg gcatctgcgc
      901 cgacaaccgc ggattgagcg gcggtggcaa cgtcacccgc agcggcctct tcatcatcag
      961 cagcgacggt tcgtgctcca ccagaaacac aaacggcttg cgagtcatcg tcgccagcac
     1021 cgtctccagc gcttgtcgca tctcgtcggc gctctggtag cgatcggctg gcgacttttc
     1081 catcgcccga cgcaaaatcg ccaccacact ttcgggcacg tccggtgaaa tgtcgcgcgg
     1141 gtccggcgtc ggtgccgtca catgcttgaa catgatctgc ggcgccgcat ccccggtgta
     1201 cggcggcttg gccgtcagca gcgtgtaaaa cgtcgcaccg agcgaataaa tgtcactgcg
     1261 gcgatcgacc tcgccatcgc tggcttgttc cgggctcatg taaagcggcg tgccagcgac
     1321 gacaccagtc tgggtcagcg caccttcgtc aaaatgcaca tctttgacga gtccaaaatc
     1381 gaccagcttg gtcgtgccca tcggcgaaca gaggatgttg tctggcttga tgtcgcgatg
     1441 gaccagaccg gcctggtgcg cagcgtgcag cgccgagcag cagtccatga tgatcttgca
     1501 ggcctcggcg tagtggagcg gccctttttc cttcacatgg ctcgacgctg acgaaggatt
     1561 cagcagctcc atcacgatgt agtagtgctc gtcctcctga ccaatgtcat agatggcgat
     1621 gatgttggga tggtttagcc tgcctgcgac ctgtgcctcg gtgatgaagc gctcgaccgc
     1681 tttggggctc gacgtcagcg actcgggcag aaacttgatt gcaacccgcc ggctcaacac
     1741 ggtgtcttcg ccctcgtaga cgagacccat gccgcctttg ccgatgaccg cttcgacgcg
     1801 gtatttgccg aacatctgac cgaggcgggc gtcgttgcga cgcgacagcg aaggctcaga
     1861 ttgggtgggg accgccattc caaccgggat gtctatcgtg tccgatattt tctcgtcgga
     1921 aacgctgccc gtgccaggcc cccccaggcg attgccaggg gtctgccgtg atcccggtcc
     1981 accgggaggc actttctgat tggggccaga catcctctct aatcatcgaa gactcgcaaa
     2041 gtccggtcaa ggcacccaaa ttggggccaa gcagtcccca agtaggcgga ctgccggcgt
     2101 gttatggtac tgaggtcgtg cgagtacatc gttccttgct tcccgtgatt gctggcctgc
     2161 cgcttggtct ggccatggca caagttccac cgccgggcgt tccccccgct gcggccactc
     2221 aagccccacc cgctgccggc acagttcaag ctgcgcccgc ggttcccgga cagcctggtg
     2281 atccaagcgc tgcggccaaa aaaccgcgca agttttggat cgacggcaaa taccgcgagc
     2341 taaaaaaagg tgaggtccgg ccttacacac cgcccaacct gccgccggaa cccgaccccc
     2401 agcccagcgc cgcgcgcgtc tttgaggtcg ccaagcccga agaaggcgat ccgcaggttc
     2461 tggctcgtcc gtattacgga tcacccatgg tcgggacggt cgtgcctggc acccgcatcg
     2521 cggtccgcgg cgaagtgatc gccaaaagca gccgctggtg tcattccaag cgctggtttg
     2581 cggtgatgcc atttggctgg atgtgcagcg atcacggcaa agccaccgac gcaccagcca
     2641 ccaccgatcc cgtctacaaa atcgtccctg gcgagcgcgt gccctatcgc tacgtgatgg
     2701 tttcggccca agaaatgccg atgtggacca gcctcgatgc gttcaaaaac aacgaagaac
     2761 ccgagcgcaa gctggccaaa ggcgattcgg tcgcgattga aaaaaacttc aagcacgaag
     2821 gcgaaaccta cctgcaaagc gtcgagggca agatcctgcc cgccaaaggc acatatcaga
     2881 tgcccgcgac gtcgcagtgg catggcgtgc tgatcgatga caaaacccat ctgccgtttg
     2941 gttgggtgct gtcggaacag ctcaaagccc aggccgcgcc ggatcaaccg acggtaacgg
     3001 gcatgctcaa gcggcgtgtc caggtcgaca tcctcgaaga gatgacggtc aagggcaagc
     3061 gctggctgcg ggtcaaaccg tcggcggcac cgaccaaaga gttcccgctc gcgaccgagc
     3121 cgctgccccc gcccccaatc cccgtcgcag ctccagggac catcgccacg gcagccaaac
     3181 cagcgacccc gacgcccgcc gcaccggtgg ttacgccaac ttcccccgct gtagccgtgg
     3241 ccccggcaac tccagcccct gcggggacag ccgccacacc gcccgcacct cccgtcgagc
     3301 aagcgatgtg ggtgccggcc tgggcggtga acgaagtccg gcagatcgcc aagccagcgg
     3361 gaatcggggt caatgcgcag tggtttgatg ccgatctgtc ggaacaagtg ctcgtcgctt
     3421 acgaaaacga caagccggtc tatgcgacgc tggtttcatc cggaaaaaac aacgccacgc
     3481 cgcttgggat ttatcccgtc tgggcgcgcg tcgcggcgat cacgatgaag agccagcctt
     3541 acgacgacaa gccgtacttt gtgaacatgg tgccgtggtc gacgtttttt caagcgcaca
     3601 acgccatcca cggcgcctac tggcacgatc gcttcggcta tgtgaagtcg cacggctgca
     3661 tcaatgtggc gccgctcgat gcgcgctatg tgtttgaact gctaaagccc gcagtgccac
     3721 cgggatggac atcggttcgc ccgacggacg tgacgcagtg gccgatgttg tacgtgcatg
     3781 attcgggacg gcggcccgcg ttccatcaag atcgcccgat cggtgcgccg gatcgcgaag
     3841 acgaaagcga gcgcttcgaa gaaaccaacc gactgcgcat cgagcaaggt ctgccgccgc
     3901 tgccgccacc tggaaccaaa ccgcccgcag ccgcaacgcc cggtgtgccc gccgctgcgg
     3961 ccccggttcc tgctgccgcc cccgcgccgg tagccaaccc agcgcttgca ccgcgctgac
     4021 agcggcgacc ttcccgccca gaacccgcac gctcgtgaga cagactgcta gagcgtcggc
     4081 cattgacggc acaacctgcc acaaagtagc ctggctgcga tgatcaaggt acgcgcgttt
     4141 ccgtggctat tgtctttggc tttctcgacg gtatcgctga ctggctgccc cgagcaagcc
     4201 gaacagccgc aagcgaccgg gccaaaacct gacaccgtct gccccaaaga actgggcgct
     4261 tcatgcaagg tgttaaaaga cgagttcgat gaaaagaagc tcgtcgctga gtatcacgtc
     4321 ctggtgtcgc ccgataccaa gcacgacgac gcggacaact acctcaaagc gctgtaccgc
     4381 cacatgatga cccgtcgcga cgtggcgccg acgcagcttg gtggttttgt ttacacaaac
     4441 gaagcgcagt ttacgacgcc gccgttgtct cccgtgggaa gcgtgacgca gaaaccaggg
     4501 gaaaaatcgc cgacgttcga aaacaaaatt gcgctcgagc tgtggcagca ggccgaacag
     4561 gcgataaagc tctcggcgcg cgcggatcgc aagctcaagc gcaagctaga gtacgtcgct
     4621 gagccgacca aaagcatggt gacggtgatc ctgccgttta cagaaggcgc gaccgaggag
     4681 tgggcgaagg aaatcgacta ctcgcaggtg attgcctact tctccgagct gtcgcagcag
     4741 ctttttaaca acatcccgga tctgaagtcg tttttgtacc gtgcgcgctg gaaagatcag
     4801 gatgtggcgc agatcgaaat cacgcgcgcc gactacgaaa agataaacct ggctgacgtc
     4861 gagcaccgcg tcggacagct tgcgggacgg acgtttttgg aactgacgac gggcaaaggt
     4921 ggcagcgaag cctcggtcga aaaagcccac aaccgccgtc gcgccgccga atacaaaaaa
     4981 gcgctcgacc agatcaaagg cggcaaggtc atcatcaatc cgctgctcaa gtagccgccg
     5041 cagcgcatgt ttcgcgccgc gacggaacta cacgggcgag ctgggaacaa aacgtcccga
     5101 ctgcggatcg tgaacgcgct gttcgaccgt cagcttggcc gacgcccctt ggccatcaat
     5161 cgtatagacg ttgtagcgag cccgcctttc cggcagcgga tggttgtacg tccctgagcc
     5221 aacgccgatc accggaatcg aaccgtttgg cccgtcgagg gtcgcgcgca agtcgcggtg
     5281 ttcatggccg tgcagcacaa gctcgcagcc gacgcgccgg agcacatcat gaagccgtcc
     5341 gcgatctcgc agcgcccgca acacatccaa gtgctgcggc agcggtgggt gatggatcag
     5401 cagcacacga aaatgatcgc gcagcccgtc gagcatctgt tccagtcgct gaagctgctt
     5461 tccacccagc cacccatcgg cgagcggcac cggggaaggc agcgccgtcg agcagcccac
     5521 aatggcaacc ccatcgcgct tccgcacaat cggccatgtc ggcagctcgc cctcggtcgc
     5581 accgtcgctc tgtgcatagg gcaaaagcat cttttcgaac gtgtgcccaa agtacgcttc
     5641 ccagacgtag acatcgtgat tgccaggaat cgccgtcacg ccctgcggtc cctgcggcag
     5701 actgtcgagc gcttggcgcg cccgctgaaa ctcgctcggc aaagacaggt tcgtcagatc
     5761 gccggtgacc accacatgat caagcggctg ctgtctcaag tcatcgcaca gcgcgtcgag
     5821 cagaaagctg ggatgggccg cccgtcgctt gcgcaggagg ttcagccaac caaaaaatcg
     5881 cttgctgaga aaacgaagcg gcgacgcccc cgacaggtcg agcgcgtgca gatccgagat
     5941 gtgtgcgata cgcatgtcgg atacaaacta gcgcaaacgt cggcgctccg cccaagctaa
     6001 gatcggcttc ctcgatgacg cactcgcaag ttcgcaagct tcccgtcggt tttgtcgata
     6061 cgcttttgca aattgtcggt ccgtccggac ttatcaccga cgcagaagcg atcgagccat
     6121 ttttgaccga gccacgcggt acgtatcgcg gccaaaccga cgtggtggtt cggcccgcat
     6181 caaccgagga acttgcttct gtcgtcgctg cgtgcgcaaa cgcgcgggtg ccgatggttc
     6241 ctcaaggcgg tggaaccgga ctgtgcggtg gagccgtgcc agccccgtcg ggggaacaga
     6301 ttgtcgtaag ccttgcgcga atgaatcgaa tccgagcgct cgatgccagc agtttcacga
     6361 tgaccgtcga agccggctgc atcttggccg acatccagcg cgccgccgaa gaagccggac
     6421 ttttgtttcc cctgagcctg ggggcggaag gcagctgcca aatcggcggc aatttgtcga
     6481 cgaatgccgg tggaacccaa gtgctgcgct acggcaacag ccgcgatttg gtgctggggc
     6541 tggaagtggt gctgccaagc ggcgagatcc tcgacatgct gcgcggcctg cgcaaagaca
     6601 acactggata tcacctgtcg gggctgttct gcggggcaga aggaacgctg ggaattgtct
     6661 cggcggcggt gctaaagctg ttcccaaagc cacgcgaagt cgtgacggcg cttttggcgg
     6721 ttcccagtct ggacgcggtg ataaagctgc tttcgctggc gcgtacagcg acgggggacc
     6781 agctttcagg ctgcgagttt attcctcgca ttgctctcga catggtgctt cgccacatac
     6841 ccaatacccg cgatccgttc ccgacgacgt atccgcacta tctgctgatc gaagcgcaga
     6901 gcggcagcga agacggacgg ctcgggcggg aagtggaaaa gctgctggcc gacgctgccg
     6961 acgccgatct gatcctggat gcgacggtgg cgcaaaatcc cagccaagcc gacgcgctgt
     7021 ggcacatccg cgaaagcatt cccgacgccc agaaacccga aggtctgtcg gtaaagcacg
     7081 atgtgtcgat cccgatccat cttgtgccga agttcgttgc ggaagcgacg cggctcgtcg
     7141 aagcggaagt ccccggcatt cgggtggtcg cattcggtca tgtgggcgac ggcaatgttc
     7201 acttcaactt gagccagccc gtcgggatgg atcgcgcaac ctatgcgcag caccgcgagc
     7261 agctatctca cagggtccac gacctggcga tgtctttggg cggcagcttc agcgccgagc
     7321 acggcatcgg tcagctcaag cggcaggaac ttgtccgcta tcgatccgcc gtcgagcttt
     7381 cggtgatgcg caagatcaaa gccgctctcg atccgctcgg tctgatgaat cccggcaaag
     7441 tgctgtagtc cgacctttgg cagaagtgcc tgccgtcgaa ttccgtcgcc caccgtgaca
     7501 ccccagcgat cgggatacgc agcttttttg gcagcgcggc gatcttggcc aaaaattggc
     7561 ccaccatgaa gttgtcccaa ctgctccgcg aggtggcgct gcgcccgaat gcggccacgg
     7621 cattttcgac ggagacggac ctggatgcga tcgagatagg cgatgtccgc gaggactcgc
     7681 gacaggtcca gccaggcgat ctgttcgtcg cggtgccggg tcagaccgtg gacggtcatc
     7741 acttcttaag cgatgcggca cagcgcgggg ccaaagcagc gatcgtcgag cagattcgac
     7801 ccgactgcaa actgatccaa attccggtag aaaacgcgac ggaagccctg gcgcaactgg
     7861 cgatgaaccg ctacgggcgt ccgtcggatt cactcacgct ggtcggaatt accggcacca
     7921 atggcaaaac gaccagcaat tttctcgtcg aaacgctgct cgaagaagcc gacttgcgac
     7981 cgggactttt gggcacggtg ctgtatcgcg gaccaggtgt gagccagccg gcgccgttta
     8041 caaccccgac ggcactgctt cttcatcaga cgctggccgt gatggcggaa catggcgcca
     8101 aagcggtcac gatggaagtg tcgtcgcacg cgctggcgct tggtcggctt ttcggtgttc
     8161 gttttcgcgt cgctgcgttc accaacttaa cccaagatca cctggatctg cacggcacga
     8221 tggacgagta ttttgccgcc aagtgccagc tctttcatcg ccacctcttg cccgccgagc
     8281 gaggtggtcg cgcagtgata aacgtcgacg atccgtgggg ccgaaagctg tttgattcgc
     8341 tgccgccgga acagcggatc ggcttttcgg tcgacggtcc agccgacatt tcgatcgcaa
     8401 gcgagcgcac cattgtcgag ggaatctcgg cagtgttccg caccccgctc ggggatgtga
     8461 cgattcggtc gccgctgacg ggtcgcttca atctttccaa cttggcgctc agcgtcggaa
     8521 ttggcgttgc actcggtctg tcggcggttc aaattggccg cggtctttcg cgcctgagcg
     8581 gcgtgcccgg tcgactcgaa agggtgccca gccgacgcgg cccgacggtg tttgtagatt
     8641 atgcccacac tcccgacgcg ctttcgcggg tgctgcaaac cctgcgaccg ctcacgcgca
     8701 gtggcacaaa accgagccgg cttctggtgg tgttcggttg tggcggtgat cgcgacgcgg
     8761 gcaaacgacc gctgatgggc caggccgccg cagaagaagc cgatctggtg atcgtcacgt
     8821 cggacaatcc acggactgaa cagccacagc gcatcctcga catgatcgtc gagggcgtcg
     8881 aaaaaacggg gcgactgccg ctgatttcga gccgatccga tctggccagc gcgacgagcg
     8941 gctatctggt cgaaatcgat cgccgacaag cgattgtcgc ggcgattttg gcggcgcagc
     9001 cggatgatgt cgtgctgctg gccggcaaag gccacgaaga ctaccaagtc atcggcacca
     9061 gcaaacagca cttcgacgat cgcgaagaag cccaaaaagc gctggccctg cgtcctgaga
     9121 cccagccgcg ttcaggcacg ccaacgccgc cggtcatgcc gaaactccaa ccttcgatcg
     9181 aactgccact ggaacgcatc ctaagcgcaa cggcgggccg tctggttcgg ggatcaaccc
     9241 cacgctttgc tgcggtcaca atcgacagcc gcagcgtcac cccaggctgt ttgtttgtgg
     9301 caattcgtgg cgatcgtctc gacggtcaca ctttcattgc gcaggcgctg caaaacggct
     9361 gcagcggtgt gctggtcgaa aaagacaagc tgcacttggt gccggatcag cccggtctga
     9421 cggtggtgga agtcgccgat accatcgtgg ccatcggtca gattgcgcgg gcgcatcgcg
     9481 aagcacccga aatcgccgcc aagctcaaag tcattggcgt gaccggatcg tcgggaaaaa
     9541 cgagcaccaa ggatctcatc gctgcgattt ttgccgcgca cgttcaggac tcgtcagatt
     9601 tcatcaagac cgacggcaac ttgaacaatc acctcggtgt gccgctgacg cttctgcggc
     9661 tgcgacccgg acagcgctat gcgatcgtcg aaatgggtat gtcggcgcgc ggtgaaattg
     9721 cctatttgac gtcgctgtgt cggcccgatg tcggcgtgat caccaacgtc gggcccgctc
     9781 acctggaaac gctgggaacg attgaaaaca tcgcggcggc caaaggtgag ctgtttgctg
     9841 gtctgtccga tggtgcgacc gcggtgtttc tcgacggcca tgccctggtg cgcgagcaag
     9901 ccatgcgggc tggtgcgctg tcgacgcggc tgcgcctggg cttggcatcg tgtggaacga
     9961 cagcggcaac ccatgcgtcg gtgcatgtcc gcttacgccg cgaggatctc gacggtcttc
    10021 acctggaaat cagctttcct cagcttccgg gcagcccgtc gcagaccctg catgtaccac
    10081 tgattggtgc gcaccaagcc gacaacgcag cgctcgcggc ggcggcggcg ctcgcgcttg
    10141 atgtaccacc tgcgacgatt gctgccggac tcgcgcgggt tacgccggga aaacaccgtg
    10201 gccagcttct gaccatcggt ggacggcgcg tgcttgatga ctgctacaac gccaatcctt
    10261 cgtcggtatc ggcggcgctg cggagccttt cgggacttcg acaaggcggc caggccatcg
    10321 cggttctggg tgacatgctg gagcttggcc cgacggaagc agagctgcat gccaaggtgg
    10381 gacgacttgc cgccacgctc ggaattggga aagtcatcac cgtcgg
//
LOCUS       DAORGN010000180        10268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2796_length_10268_cov_6.578283, whole genome shotgun sequence.
ACCESSION   DAORGN010000180 DAORGN010000000
VERSION     DAORGN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2796_length_10268_cov_6.578283"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1322)
                     /locus_tag="PKL17_16800"
     CDS             complement(15..1322)
                     /locus_tag="PKL17_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNK46443.1"
                     /translation="MIPERPDFLYKTPGRVALVLAGGAARGAYEVGVIQHIVEEVAKD
                     LGRDVPLDIICGTSVGAINGCGLAAFADAPRSRARRLVAQWQSLRMDDVVRLKPLHLA
                     KFVRTLVAPSPLATEVKEDRYGGLIEPSGMEEIIRNAIPFERITEHLRSGLLQAVTAS
                     ATHIATGNTVVFISRSSHGVPRWSRDPKVVPRKVSELKIQHALASAAIPLVFPSVRID
                     GEFYCDGGLRQNVPLSPARHLGANGMIVVSPRFVGEIQKIPERPNPKAKMPSPMFLIG
                     KALNALLLDRVENDIERLQRINQILLAGQKQFGDDFLPQLNHAMGYHHKTDGMKPLSA
                     LLVRASDNIGRMAVDYVCSPTFSKRMPGALGRLSKIICEMDAEEESDLLSYLLFDGEF
                     ATQLIELGRRDAKAVHKEFLRFFTDLPADLSPPIHPPAPENAH"
     gene            complement(1354..2217)
                     /locus_tag="PKL17_16805"
     CDS             complement(1354..2217)
                     /locus_tag="PKL17_16805"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNK46444.1"
                     /translation="MRAAQSFSIFFPMYNERDNIAATLRQTLAMIRRLGFADYEILVV
                     DDGSRDGSADVVRRFMQEDPHIRLVQHPKNRGYGAALRTGFQSARCDLVFYTDSDLPV
                     DLSVVADAIEAAQGTDVLVGFRLQRFDTPRRALYSKVYNFLVRLLYGVRVRDVNFSFK
                     FVHRRVLDKIKLTANSVFIDGQLLAEAQRHGFAISELPIVYHPRVIGSSSFNSLRAAW
                     VTLVELVTHRFSPESLQAAEPPSVESLPMPATNEAETPSWQKSEEAAMASLSRPESLE
                     TPEESPLHRVG"
     gene            2327..3427
                     /locus_tag="PKL17_16810"
     CDS             2327..3427
                     /locus_tag="PKL17_16810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNK46445.1"
                     /translation="MRIYSASQVCRVHVELTSACNAACPMCPRTRSGGQRNPNLPRAE
                     LTLADFEAMFPPDLLGGLKQFLFCGNYGDALVARDTLPIVEYLRTTNPKLRIKLVTNG
                     SGRDAVFWKKLAKLATTVTFSIDGLGETNRLYRRRTSWDRIMAAVTAYLGAGGRAEWD
                     FLVFRHNEHQVKEAEALAKSLGFAKFNLKRSQRFLVEGRAEERFPVLGQDGKVSYYLE
                     LPEQAEHQNSGFVDLSRLTGGEKSYGDYLRSTRIACKAVRDEQVYLSATGHLFPCCWL
                     GQIYDSWPSPMAEEIRSRIAELPAGLADLDLRRHGFAEIISGRFFQQLVVRGWDVTTP
                     AGRLSACGRMCGEIDTHSRQWDGPPVPPAVQK"
     gene            3461..4039
                     /locus_tag="PKL17_16815"
     CDS             3461..4039
                     /locus_tag="PKL17_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46446.1"
                     /translation="MRMSALVLVASLSGCSSGGASPHGPGTGGEDAHLPTPRDSGVTD
                     DLSLPMADLSPQDGGRVDDLAVPRDLSMMPDLAVPADLRVPADLAVAPDMSLPTSQNV
                     DIYVDNFCKMDVIPKKFDVPPGTFLKLTYINHSRDYAVDVWLSYGGGYLDLKTGTSWA
                     DRFEFCRYPRPYSAYADISTACSKYRLMINCL"
     gene            complement(4092..4556)
                     /locus_tag="PKL17_16820"
     CDS             complement(4092..4556)
                     /locus_tag="PKL17_16820"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNK46447.1"
                     /translation="MHPHLPVWVLVADASRARIFEVKIGSQVFELVSEFPHPASRAKD
                     HDVGSDRPGRVSQSHAGPHPGKGSRSGTEEHSHKETEHDHFARELVAELTRGLNEHRC
                     GRIVVVANPEFLGHLREVAGSNLAKHISESVAKDYTGLDARELAERLRDHLA"
     gene            complement(4605..5411)
                     /gene="rlmB"
                     /locus_tag="PKL17_16825"
     CDS             complement(4605..5411)
                     /gene="rlmB"
                     /locus_tag="PKL17_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNK46448.1"
                     /translation="MKAKASQGSQVVYGIHAVRELLTRRPADIHSLLIAQGSEGPSDA
                     LAKLWTVAEQAGLRPLGKSRPELDRISQGGVHQGVVALCGEFQYETDPLLPLAQALQN
                     DKTPLVLILDGVTDPQNLGALIRSACVLGADGVILPQDRSAQVTAATIKAAAGATSAI
                     PIVKVPNLVRAMEQLKEGGLWLVAAVAPGQGGQPLWQIDLTAPIGLVLGGEGPGLRPL
                     VRKTCDLRAEIPMPTGLHGASLNVSAAGAVLLYECLRQKSGREACIPAHP"
     gene            5476..6486
                     /locus_tag="PKL17_16830"
     CDS             5476..6486
                     /locus_tag="PKL17_16830"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNK46449.1"
                     /translation="MVTPSDAEESATPIVTPLLLLTGFLGSGKTTLLNRLLSSPGHPR
                     IGVVVNEFGQVGIDGKLLPGTGIMELANGCVCCVKGTELWESALELVDRAAAEVLIVE
                     TSGLVEPQALLTQYELLPSRLADRLSLRGLLCVVDALFVHEAVHRRPEAKQQIELADR
                     LLISKLDLASADDLLKVHGLLDSLSATTDRVGVSLGSTDLEIAEVLRWAMAPLAKKPV
                     RPRPASGARHGGTQLSAVSVRLPFPLLLLPLRRLLDELPGDVLRAKGFVWLYDPARKE
                     KTLHVVQLAGHRVELTAASDEVRAATGEDGALVFIGERLDESWLRLRLSACAATLLTK
                     AD"
     gene            6519..8126
                     /locus_tag="PKL17_16835"
     CDS             6519..8126
                     /locus_tag="PKL17_16835"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HNK46450.1"
                     /translation="MKRAHLLVAALPLASLACEPSQQPESPPRYVPSPPNMPRLTTAQ
                     YRSSLQDVFGGVLPTTALEPDTNPYLFYSIGAATTTLSEQGTQQYAESAAVIADALFA
                     DAKRRDALIGCTPKTATDPCVSGFVSRIGRRLFRRPLLAEDVARWTSVVESTAEGDIY
                     RGLRLAVFGMLQSPRFLYRVEVGEPDPQDPSRRRYDSFEMAERLSFLLWNTAPDEELL
                     QAAERGELLDDASLERQARRLLDSPRAQSAVQGFFDQYLNLPALSQVERDPARYARFS
                     KTLLASMRTEVQLLVDDLVFRHETDIRSLFSTRRTFVNKELAALYDVSAPQASEVAFV
                     PTELPESGARAGVLSLGAVLTLNAHPTETSPTLRGKYLRERVLCQLVPPPPGNINLDL
                     TKMEGQPRTLRERLEEHRKNPACYSCHAIMDPPGFLFENFDSIGAFRTMAAGLPVSAQ
                     SDFEGESLGNASELAARLGSDPRVARCLVRQVFRHASARLDSEGDDPVIVNLTSQFAD
                     SGYRFRELLVALLKSEGFRFAQGEVTP"
     gene            8123..9499
                     /locus_tag="PKL17_16840"
     CDS             8123..9499
                     /locus_tag="PKL17_16840"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HNK46451.1"
                     /translation="MKRFQLSRRSFLRGALGGVAISLVLPPLEAMLDHNGAYADGGGE
                     EPFFGLFYWANGLPWNAKHGAMQASAGKDDLWTPMKTGPDYAPSPLLLPLARHKVCVA
                     TGLEPKTEIPPVPPGQGDGHMRGFMVAMTGDRVRSEGFDHPSHTLTALRPTLDQLVAK
                     HPAFYKTAPRFRSLQVGVSRARFHEYGHWNAISYNGPDSLNPPILSATELFDKLFAVP
                     LETAEQGRRAKVLDAVLDDAKRLRRGLSARDAARLDSHLDQIAEIQRRLSQSSVSCKV
                     PGRPSDDADLHKKTTAMADLLAIAVNCGLTKAFSFMLTSPATTHVFSNLGVGDDMHTT
                     CHAGRWDDIRKIAQYQMEAFALFLDSFAKIKLPSGQTLLDRACIYGTSEYGEGWQHGE
                     KEHPLILAGGSCGRLLRGVHTREPGGNLCKAQLTALKALGLPFDKFGFSGAETNSTVA
                     GMLAQGVT"
     gene            9742..>10268
                     /locus_tag="PKL17_16845"
     CDS             9742..>10268
                     /locus_tag="PKL17_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46452.1"
                     /translation="MIASCKDGYYDADKDVKNGCETTSTCVADADCKTSCDSIGKTVC
                     QSGKESCVPPVETCNAKDDNCNGACDEGGLAGCRVGVHRAVGKGHFYTTDLKAAMSPP
                     FSLEAANYFYLYVGPSPGLSALYLCPKPGGMWFLTTDARCEVLNVDGTPLGFVSTDAR
                     CGATPLYRMYSPASG"
BASE COUNT         1803 a         3079 c         3355 g         2031 t
ORIGIN      
        1 gtccggcggt ttgcttagtg ggcgttttcg ggagctgggg gatgaatcgg tggggacagg
       61 tcggcgggca gatcggtgaa gaagcgcagg aactctttgt gtaccgcttt ggcatcgcgg
      121 cgacccagtt caatcagctg ggtggcgaac tctccgtcaa acagcaggta ggacagaagg
      181 tcgctttcct cttccgcatc catctcgcag atgatcttgg acagtcgtcc gagcgccccc
      241 ggcatgcgct tcgaaaacgt cggtgaacag acgtagtcga ctgccatccg tccgatgttg
      301 tctgacgcgc gcaccagcag cgccgacagc ggtttcattc catcggtctt gtggtgatag
      361 cccatcgcgt ggtttagctg cggcagaaag tcatcgccaa actgcttctg cccggccaag
      421 aggatctggt ttatgcgctg aagccgctcg atgtcgtttt ccacccggtc gagcagcagt
      481 gcgttgagcg ccttgccaat cagaaacatc ggacttggca tcttggcttt ggggttcgga
      541 cgctcgggga tcttctggat ttccccgacg aaccgcgggc tgaccacgat catgccgttg
      601 gcccccaggt ggcgagctgg cgacagcggc acgttttggc gaagtccacc atcgcagtag
      661 aactctccat caatgcgcac cgatgggaac accagcggaa tcgccgccga tgcaagcgcg
      721 tgctgaattt tcagttccga aaccttgcgc ggcaccactt tgggatcgcg cgaccagcgg
      781 gggacaccgt gactggaccg cgagatgaac accacggtgt ttccggtcgc gatgtgggtg
      841 gccgaagctg tcaccgcttg cagcagaccc gaccgcaggt gttcggtgat tcgctcaaat
      901 gggatcgcgt tgcggatgat ttcttccatt cctgacggct cgatgagccc cccgtagcgg
      961 tcctctttga cttcggttgc caatggcgag ggggccacca gggtgcggac aaacttcgcc
     1021 aggtgcagcg gctttagccg caccacgtca tccattcgca gcgactgcca ctgcgcgacc
     1081 aagcggcggg ctcgactgcg cggcgcatcg gcgaacgcag cgaggccaca gccattgatt
     1141 gcgccgaccg acgtgccaca gatgatgtcg agcggcacat cccgacccaa gtccttggcg
     1201 acctcttcga cgatgtgctg gatcacaccg acttcatacg caccacgggc cgcaccaccc
     1261 gcaagcacca gcgccacccg accaggggtc ttgtagagga agtccggacg ttcggggatc
     1321 atctcctcat tttcgaggag tggggagctg ctttcaaccg actcgatgca gtggcgattc
     1381 ttcgggggtc tccagcgact caggccggga caaagacgcc atggccgctt cctcgctttt
     1441 ctgccagctt ggggtctcgg cttcgtttgt cgccggcatt ggcagggatt cgacagatgg
     1501 tggttccgcc gcctggagac tttcgggaga gaagcggtgg gtgacaagct ccacaagcgt
     1561 cacccacgcg gcgcgcagcg agttgaagct ggatgagcct atgacccgag ggtggtagac
     1621 gatgggcagt tcgcttatcg caaagccatg acgctgcgct tccgcgagca gctgcccatc
     1681 aatgaacacc gagttggcgg tgagcttgat tttgtcgagc acgcgccgat ggacaaactt
     1741 aaacgaaaag tttacgtccc gaacccggac cccatacagc agccgtacca ggaagttgta
     1801 gaccttcgag tacagggctc gtcgcggtgt atcaaagcgc tgaagcctga agccgaccag
     1861 aacatcggtg ccttgggctg cctcgatagc gtctgccacc accgataggt cgaccggaag
     1921 atcgctatcg gtatagaaga ccaggtcgca gcgtgccgat tggaagccgg tccgcagggc
     1981 ggcaccatag ccgcggtttt ttggatgctg aaccaggcgg atgtgggggt cttcttgcat
     2041 gaagcgccgc accacatctg ccgatccatc ccggcttcca tcgtcgacca ccaggatttc
     2101 atagtcagca aagccgagcc gacgaatcat cgcaagcgtc tgccggagcg tcgccgcgat
     2161 gttgtcccgc tcgttgtaca tcgggaagaa aatcgaaaag ctttgtgccg cccgcacgcc
     2221 ccggatgtag cgcgaccttg ccgccgcgtc tatggaaaac gagcacttgg gtgccccatt
     2281 tgccgcggaa aaccggctac gctaagcacg acccttggcg taagacatgc gcatctactc
     2341 tgccagccag gtctgtcgcg ttcacgtcga gctgacctct gcctgcaatg ccgcctgtcc
     2401 gatgtgtcca cgcactcgat cgggcgggca gcgaaacccc aacctgccgc gggccgagct
     2461 tacgctggcc gacttcgaag cgatgtttcc gccggacctg ctcggcgggc tcaagcagtt
     2521 cctgttctgc ggcaactacg gcgacgcact ggtggcgcgc gacaccctgc cgatcgtgga
     2581 atacctgcgg acgacaaatc ccaagctgcg aatcaagctg gtcacaaacg ggtcgggacg
     2641 cgacgctgtg ttctggaaaa agctggccaa gctggcgacc accgtcacgt ttagcatcga
     2701 cgggcttggg gaaaccaatc ggctgtatag gcgccgtaca agctgggatc gcatcatggc
     2761 ggcggtgacg gcgtatctgg gggcaggtgg tcgggccgag tgggactttt tggtctttcg
     2821 ccacaacgag catcaagtca aggaggccga agcgctggct aagagtctgg gctttgccaa
     2881 gttcaacctc aagcgctcgc agcggttctt ggtcgaaggt cgtgccgagg agcgatttcc
     2941 ggtgctgggg caagacggca aggtgtctta ctacctggaa ctgcccgagc aggccgagca
     3001 ccaaaacagt ggctttgtcg atctgtcgcg gcttacgggg ggcgaaaaaa gctacggcga
     3061 ctacctgcgc tcgacgcgca tcgcatgcaa ggcggttcgc gatgagcagg tctatctgag
     3121 cgcgaccgga cacctgtttc cgtgctgctg gctggggcag atttacgact cgtggccttc
     3181 gccgatggcc gaagagatcc gcagccgaat cgccgagctg cccgccggct tggcggacct
     3241 ggatctgcgt cgccacggct ttgccgagat catctctgga cgcttttttc agcagcttgt
     3301 ggtgcggggc tgggatgtga cgacgccagc gggccggctg tcggcgtgcg ggcggatgtg
     3361 tggcgagatc gatacccaca gccgtcagtg ggatgggcct cctgtgccac cggcggtgca
     3421 aaaataatca cctcacagat cgcgtggtag gcttcgcgcc atgcgcatgt cggctcttgt
     3481 gctagtggct tcgctttcag gctgtagctc gggcggggcg tcgccgcatg gtccaggcac
     3541 aggtggcgaa gatgcccacc tgccgacgcc gcgggacagt ggagtcaccg atgacttgtc
     3601 gctgccgatg gctgacttgt caccgcaaga cggcgggcgc gtcgatgact tggcggtgcc
     3661 gcgcgacctc agcatgatgc ctgaccttgc cgtgccagcg gatttgcggg tgccggcgga
     3721 cttggcggtg gcgccggaca tgtcgctgcc gaccagccag aacgtcgaca tctacgtcga
     3781 caacttctgc aagatggacg tgattcccaa aaagttcgat gtgccgccgg ggacgttcct
     3841 gaagctgacc tatataaacc acagtcgcga ctatgctgtg gatgtctggc tgtcgtatgg
     3901 cggtggatat ctggacctga agaccggtac gtcgtgggcg gatcgtttcg agttctgtcg
     3961 ttatccgcgg ccctattcgg cctacgccga catcagcacc gcgtgctcga agtaccgact
     4021 gatgatcaac tgcttgtgac ggtggggcca gcccgctttg gcttcgcggc tggccgtttg
     4081 agcggggatg cttaggccag gtgatcgcgg agccgctcgg ccagctcgcg ggcgtcgagt
     4141 ccggtatagt ccttggcgac gctttcgctg atgtgcttgg ccaggttgct gccagcgact
     4201 tcgcgcaggt gtcccaggaa ttcggggttg gcgacgacga cgatacgtcc gcagcggtgc
     4261 tcgttgagcc cgcgggtcag ctcggcgaca agttcacgcg cgaagtgatc gtgttcggtt
     4321 tccttgtggc tgtgctcttc agtgcccgag cggctgccct tgccggggtg cggtcccgcg
     4381 tgggactggc tgacgcggcc cggacggtcg ctgccgacgt cgtggtcctt ggcccggctg
     4441 gccggatggg ggaactcgct gacgagctcg aacacttggc tgccgatctt gacttcaaag
     4501 attcgggcgc ggctggcatc tgctaccaag acccaaacag gaagatgggg gtgcatcatt
     4561 gtctccttga ctgtgacgga caaactgagc gtcggcagtg tgccttaagg gtgcgctggg
     4621 atgcaagctt cccgaccgct cttttggcgg aggcattcat acagcagcac cgccccggct
     4681 gcggagacgt tgagcgatgc cccatgcagc ccggtgggca tgggaatttc agcgcgcagg
     4741 tcgcaggtct tgcgcacgag cggtcgcaga cctgggcctt cgccgccgag gaccagaccg
     4801 atgggggcgg tcagatcgat ttgccacagc ggctgtccac cttgtcccgg cgcaactgca
     4861 gcgacgagcc acagcccgcc ttctttgagc tgttccatcg cccgcacgag gttgggcacc
     4921 ttgacgatgg gaattgctga ggtggctccg gcagcggctt tgatggtcgc tgcggtgact
     4981 tgtgccgagc gatcctgcgg caaaatcacg ccatctgctc ccagcacgca ggccgagcga
     5041 atcagtgccc ccaggttctg tggatcggtc acgccgtcga ggatgaggac aagcggcgtc
     5101 ttgtcgttct gcagggcctg cgcaagcggc agaagcggat cggtctcgta ctggaactcg
     5161 ccacacagcg cgaccacgcc ttggtggact ccgccttggc tgatgcggtc aagctcaggg
     5221 cgggacttgc caagcggacg cagacccgct tgctcggcca cggtccaaag cttggccagc
     5281 gcatcgcttg gaccctcgct gccttgggcg ataagcagcg aatgaatgtc ggcaggccgg
     5341 cgcgtaagta gctcgcgcac ggcatggatt ccgtagacaa cctgacttcc ctgcgaggct
     5401 ttggctttca tatggggcac cttgtaccgc actgcttggc gagccggggc ggttttgtgt
     5461 atagcttgcg cccccatggt cacgccgtcc gacgctgaag agtctgctac cccgattgtt
     5521 acgccgcttc tcctgctcac gggttttttg ggcagcggca agaccacgct tttaaatcgt
     5581 cttctgtctt caccgggtca tccgcgcatc ggcgtggtgg taaacgagtt cggacaggtg
     5641 ggcatcgacg gcaagctgct tcctggcacc gggatcatgg agcttgcaaa cggctgcgtc
     5701 tgctgcgtca aggggaccga gctatgggaa tcggcgctgg agcttgtcga ccgggccgca
     5761 gccgaggtgc tgatcgtcga aacgtccggt ctggtcgagc cgcaggcgct tttgacccag
     5821 tatgagctgc tgccgagccg cctggccgac cggctttcgc tgcgtggtct tctgtgcgtc
     5881 gtcgatgcgc tgtttgttca cgaggcggtt catcgtcgtc ccgaagcgaa acagcagatt
     5941 gagctggcgg atcggttgct gatcagcaag ctcgacctcg cctcggcgga cgacttgttg
     6001 aaagttcatg ggttgcttga ctcgctgtcg gcaacgaccg accgcgttgg cgtgtcgctt
     6061 ggctcgacgg atcttgaaat cgccgaagtg ctgcgctggg cgatggcgcc gcttgccaaa
     6121 aagccagtgc gtccgcggcc tgccagcggg gcacggcacg gggggacgca gctttcggcg
     6181 gtgtcggtgc gcctgccttt tccgctcttg ctcttgccgc tgcgccgtct gctcgatgag
     6241 ctaccggggg acgtgctgcg ggccaagggc tttgtctggc tctatgaccc ggcgcggaag
     6301 gaaaagacgc tgcatgtcgt gcagcttgcc ggccaccggg tcgagcttac cgccgcgagt
     6361 gatgaggtgc gggcggcgac gggtgaagat ggagcgctgg tgtttatcgg ggaacggctc
     6421 gacgaaagct ggctgcgcct gcggctgtcg gcgtgtgcag cgacgctttt gacaaaagcg
     6481 gactaggaaa ccgcggacat ttcgatatgt tgcggtggat gaagcgagcg cacctacttg
     6541 tggcggctct gccgcttgcc tctttggcct gtgaaccgtc acagcagcct gagtccccac
     6601 cgcgctacgt gccgtccccg ccgaacatgc cacggctgac gacggcccag taccgcagtt
     6661 cgctgcaaga tgtctttggg ggcgtactgc caacgacggc gctggaacct gacaccaatc
     6721 cctatctgtt ctattcgatc ggggcggcga ctacgacgct gtccgagcag gggacgcagc
     6781 agtacgctga gtcggcagcg gtgattgccg atgcgctgtt tgccgacgcc aagcgtcgcg
     6841 atgcactgat tggctgcacg ccgaagacgg cgaccgatcc ctgcgtgagc ggctttgtct
     6901 cgcggatcgg gcgacggctg ttccggcggc ctcttctggc tgaagatgtt gcgcgctgga
     6961 cttccgtcgt tgaaagcact gccgaagggg acatctatcg cggcctgcgg ctggcggttt
     7021 tcgggatgct ccagtcgccg cgctttctgt accgcgtcga agtcggcgag cccgacccgc
     7081 aagacccatc gcggcggcgc tacgactcat tcgagatggc cgagcggctg tcgttccttt
     7141 tgtggaatac cgcgccagat gaagagctgc tgcaagctgc cgagcgcggc gagctgctcg
     7201 acgatgcgag cctggaacgg caggcgcgac ggctgcttga ctcgccgcgg gcacagagtg
     7261 cggtccaggg cttttttgat cagtacctga acctgccggc gctttcccag gtggagcgcg
     7321 acccggcccg ctacgcgcgt ttttccaaga cgctgcttgc gtcgatgcgc accgaggtac
     7381 agcttctggt tgacgacctg gtgttccgtc atgaaaccga cattcgttcg ctgttttcga
     7441 cccggcgcac gtttgtgaac aaagagctgg cggcgctcta cgacgtgtcg gcaccgcagg
     7501 cgagcgaagt ggcgtttgtg cccacggagc tgccggaatc gggggcccgc gctggcgtgc
     7561 tttcgcttgg ggcggtgctg accctgaatg cgcatccgac cgagacctcg ccgacgctgc
     7621 gtggcaagta cctgcgcgag cgtgtgctct gtcagctggt gcctccgcca cccggcaaca
     7681 tcaacctgga cctgaccaag atggaaggcc agccgcgcac gctgcgggag cgactggagg
     7741 agcaccgcaa gaaccccgct tgctacagct gccacgcgat catggacccg cctggctttt
     7801 tgtttgaaaa cttcgactcg attggcgcat tccgcaccat ggcggcgggt ctgccggtca
     7861 gcgcgcagag cgactttgag ggggaatcgc ttggcaatgc gagcgagctt gcagcccggc
     7921 tcgggtcgga cccgcgggtg gcgcgctgtt tggttcggca agtgtttcgt catgcgtcgg
     7981 ctcgactcga cagcgaaggg gatgatccgg tcattgtgaa cctgacttcg cagtttgcgg
     8041 acagcggcta tcgcttccgc gagctgctcg tcgcccttct gaaaagcgaa ggattccggt
     8101 tcgcgcaagg tgaggtgacg ccgtgaagcg cttccaactg tctcgtcgta gctttttgcg
     8161 cggtgccctc ggcggggtcg cgatttcgct tgtcctgcca ccgctcgaag cgatgcttga
     8221 tcacaacggg gcctatgccg atggcggcgg ggaagagccg ttctttgggc tgttttactg
     8281 ggccaacggc ctgccgtgga acgccaaaca cggcgcgatg caggcgagcg ctggcaaaga
     8341 cgacctgtgg acgccgatga agacaggtcc agactatgcg ccaagcccgc tgcttctgcc
     8401 gctggcccgt cacaaggtct gcgtcgccac gggtctagag cccaagaccg agattccccc
     8461 agtcccgcca gggcagggcg atggtcacat gcgcggcttc atggtcgcga tgacggggga
     8521 ccgggttcga tccgagggct ttgaccatcc gtcgcacacc ctgacggcgc tgcgtccgac
     8581 gctggaccag cttgttgcca agcacccagc attttacaag acggcgccgc gcttccgctc
     8641 gctgcaggtt ggggtgagcc gggcccgctt ccatgaatac ggtcactgga atgcgatttc
     8701 ctacaacggt cccgactcgc tgaatccgcc gatcctgagc gcgaccgagc tgttcgacaa
     8761 gctgtttgcg gtgccgctgg agaccgctga gcagggccgc cgcgccaaag tgctagatgc
     8821 cgtgctcgat gatgccaagc gactgcggcg tgggctctcg gcgcgggatg cggcccggct
     8881 cgacagccac ctggaccaga tcgccgagat tcagcggcgg ctgagccaga gcagcgtctc
     8941 ctgcaaggta cctggtcgac caagcgatga cgccgatctg cacaaaaaga cgaccgcgat
     9001 ggcggacctt ttggcgatag cggtcaattg cggcctgacg aaagcgtttt cgttcatgct
     9061 gacctcacca gcgacgaccc atgtgttttc caacctgggc gttggggatg acatgcatac
     9121 gacgtgtcac gcgggccgct gggatgacat tcgcaagatt gcgcagtatc agatggaggc
     9181 gttcgcgctc tttctcgact cgtttgccaa gatcaagctg ccgtcgggcc agacactgct
     9241 cgatcgcgcc tgcatctacg gcacaagcga gtacggggaa ggctggcagc acggcgaaaa
     9301 agaacacccg ctcatcctgg cgggcggttc gtgcggacga cttctgcgcg gcgttcacac
     9361 ccgcgaaccg ggcggcaact tgtgcaaagc gcagcttacc gcgctcaagg cgctgggtct
     9421 gccgtttgac aagttcggtt ttagtggcgc agagacaaac agcaccgtcg ctggcatgct
     9481 tgcgcagggt gtgacctgat gcgggccggt tcgccagtcc ttatcggggc gctctgcggc
     9541 ctcgtgggcg catggtcgtg tgcgccgccc gtggtcccgc atcctgcgaa agacaaaacc
     9601 gagacgccga ccgattgcgg tgcgcttgtg cgctgcggtt ccacctgtgt cgacctccag
     9661 cgggatccga caagctgcgg tgcgtgcgac cgcatctgcg tgatcgcgaa cgcccatgcg
     9721 ggctgtcaaa aaggcgactg cgtgatcgcg tcttgtaagg acggctatta cgacgccgac
     9781 aaagacgtaa aaaacggctg cgaaaccacc agcacttgcg tcgccgatgc agactgcaag
     9841 acaagctgtg acagcatcgg caagacggtc tgccaaagcg gcaaagagag ctgcgtgcct
     9901 cccgtcgaga cgtgcaacgc caaagacgac aactgcaatg gggcctgtga tgaaggtggg
     9961 cttgcgggct gccgcgtcgg cgtacaccgt gccgtcggca agggtcactt ctatacgacg
    10021 gacttgaaag cggcgatgtc cccaccgttt tcgctggaag cggccaacta tttctatctg
    10081 tacgtcggcc caagtcctgg gctttctgcg ctgtatctgt gcccgaagcc cggcggcatg
    10141 tggtttctga cgaccgacgc aaggtgcgag gtgctaaacg tcgatggcac gccgcttggg
    10201 tttgtatcga ccgacgcgcg ctgtggcgcg accccgcttt atcggatgta ttcgccggcg
    10261 tcggggga
//
LOCUS       DAORGN010000181        10235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2808_length_10235_cov_6.375639, whole genome shotgun sequence.
ACCESSION   DAORGN010000181 DAORGN010000000
VERSION     DAORGN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_10235_cov_6.375639"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PKL17_16850"
     CDS             <1..1224
                     /locus_tag="PKL17_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46453.1"
                     /translation="TGKRRGPLAENNKPGGSNVNDLRARLGVKKAGGGPAPAGPGGPG
                     VPAPAPIPGMAAGPGPGAPPGAVPVPPGMVPPPAGVPAPPGFAAPVEPPKPDPYAAQQ
                     AAMAASLAAFYGAGQSLPGNADEVKDGVGKQRPLGLIIGIGVAGVLGVLVGYTVNFIK
                     GQNELIELGTERAKDIKKEVESVRGRVKAALDAFKETKPGEAPSNETLDKLANLDLTE
                     PDATAKLFSPLLGTFNGKLVRSMFDYYNNVTAFFREVQLHAALTKNDRDVLAKCMKAG
                     AEAAAKGQPPLGVTFDYSQKLPQAILATLGGVVCPKEGETSCDPGEMKLRYRTSMSGQ
                     FQEKPAKGLPKDIVIPIQPTELLKNVAVGDPNQLACKDYVRRIERIRQTVTKIPELEK
                     FVIEGLDDRIKNPPK"
     gene            1544..2524
                     /locus_tag="PKL17_16855"
     CDS             1544..2524
                     /locus_tag="PKL17_16855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46454.1"
                     /translation="MVIGGYRLLKLLGAGAMGRVYLAEHERLGRRAAVKVLDGWDPEL
                     VSRLFCEARAVAQIGDEHLVDVYDFVADPQKGVVAYVMEYLAGEDLRKLLQRERVLAP
                     SRVARLAAQICEGLDAAHKVGVVHRDLKPDNIVVVRRGGDSDFIKIVDFGIAQFAGPV
                     AHRTRAGIALGSAAYMAPEQAEGKRVDGRADLYAVGVLMFELLTGEVPFLGKTSRETL
                     EIMHVTKVPSPVGDHGAGLVTDGLAKLVQRCMAKRPEERFQTADELRKALLDIAEIAP
                     DRGDKTQVLGAVAKRKPAASLRSLPPAAVLGMAIAFAVGLGAGLVIMGIH"
     gene            2609..3574
                     /locus_tag="PKL17_16860"
     CDS             2609..3574
                     /locus_tag="PKL17_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNK46455.1"
                     /translation="MKRSVPFVWVDVFAEAPFGGNPLAVLDGSGIPEEAHLPLARELS
                     LSETTFYLPPTTPESADLRVRIFTQAKEIPFAGHPLLGTAVAVLLNGRLPFRAPQTTV
                     RLETGMGVVPVTVTGSDLPERAQFSHDRISVPGTPISDERLAWLSPALGLSMDRFRTR
                     VELSSASGERILYPQVVSGGARQLIVPIQSVEEIEELSASYRDVVVAEKALGAELGIL
                     SFAFRSPPRASEPVRVRARFFAYDAPNEDPATGSAAASLAVYLHHHRLLAPGQALEID
                     QGPPRGPIEGAPGRRSLLRAFCDGTTVQVEGRVREVLQGDMRLQF"
     gene            complement(4136..4879)
                     /locus_tag="PKL17_16865"
     CDS             complement(4136..4879)
                     /locus_tag="PKL17_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNK46456.1"
                     /translation="MAKVQKVRIGELDVVIAGGTDGMGGGEGPLVVLLHGFGAPGTDL
                     VGLAPYLRAPRDTRFAFPAAPIALSSIPEMGFGMMDSRAWWLIDIERIQMDLMARRFD
                     RMAAEVPAGLADARDKICNTLDLLEKGLRVPEGKTVLGGFSQGAMLSADVMLRAGRKL
                     AGVLLWSGTIIAEEEWLSRLPGAHGLPILQSHGVMDPILPFEQAERLRDHLQGAGAKL
                     DFVAFRGGHEIPDLVVDKTSAFLQRVFLP"
     gene            5019..5561
                     /locus_tag="PKL17_16870"
     CDS             5019..5561
                     /locus_tag="PKL17_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNK46457.1"
                     /translation="MSLLPLVATSAEAADPKAAPASQPASDSIHSLSAKSIDGKAVSF
                     ADYKGKVLLIVNTASECGFTGQYAGLEALYAKYRDRGLVVLGFPSNDFHQEPASGQEI
                     AKFCKVRYGVTFPLFEKAPVSGASASPVFKFLAQKHGEPKWNFYKYLVGKNGQVLGQY
                     SSRTSPDSSDLTAAIEAALR"
     gene            complement(5558..6304)
                     /locus_tag="PKL17_16875"
     CDS             complement(5558..6304)
                     /locus_tag="PKL17_16875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNK46458.1"
                     /translation="MNEIAVQIERGCTSHSVLSAYDISQLRKSRVLAALSDEELSRLL
                     AVSQPEQITAGLIIVPQSTPIDKLLVITSGSAKVVMTGAAGREVTLEMLRPGDLIAET
                     SLLDGSPTTASVVALSDVQVLSLPRAEVVALLGRYPQLTISLLSEMANRMRRAEETIF
                     ALALQDIEERLCKMLIRLARDERSPDHQGGLLMRRRPTQQELANMVGATRESVSRCLT
                     TLARKGLAVCQGRSMLLTARLLRRDLSSAS"
     gene            complement(6465..7502)
                     /gene="obgE"
                     /locus_tag="PKL17_16880"
     CDS             complement(6465..7502)
                     /gene="obgE"
                     /locus_tag="PKL17_16880"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNK46459.1"
                     /translation="MQFIDECSIFVRGGDGGDGCAAFRREPGRPRGGPAGGDGGGGGS
                     VILIADPQRSTLLDLTYRREYRAERGQAGQGRDRYGRGGEDLIVEVPAGVVVKDSDGN
                     VLCDLATPGQRFVAAQGGQGGRGNIHFATSTWQAPEFAEPGTPGEERTLKLELKLLAD
                     VGLLGYPNVGKSSLIARVSRVRPKIANYPFTTLTPNLGVVSLPLGSRVSDRSFVLADL
                     PGLIEGAHEGHGLGHRFLRHLQRTRVLIHLLEVSNEPGREPLRDYATLNEELRRFDPG
                     LAERPQLVTLAKLDLTETRDALPTLRAQFAAQGVKLWAISAATGEGVAELMDEAYRLT
                     SSQREATSSAA"
     gene            complement(7580..7864)
                     /gene="rpmA"
                     /locus_tag="PKL17_16885"
     CDS             complement(7580..7864)
                     /gene="rpmA"
                     /locus_tag="PKL17_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNK46460.1"
                     /translation="MAHKKGQGSSRNGRDSNPKMRGVKRFDGEVVSAGNILVRQVGSV
                     YHAGRNVGLGRDYTLFALIDGKVKFETWRGNRSRVSVVPVQTDAGAASVS"
     gene            complement(7923..8231)
                     /gene="rplU"
                     /locus_tag="PKL17_16890"
     CDS             complement(7923..8231)
                     /gene="rplU"
                     /locus_tag="PKL17_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNK46461.1"
                     /translation="MYAVIEAGGKQYRVQKGDYIRVEKIAAASGDKITFPVLLVADGT
                     TVKVGQPTVASAEVTASVQGNEKGKKLIVFKYARRKGYRRKNGHRQEYTAVRIEDIRG
                     "
     gene            complement(8383..9246)
                     /locus_tag="PKL17_16895"
     CDS             complement(8383..9246)
                     /locus_tag="PKL17_16895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK46462.1"
                     /translation="MGDSPNLHGKTCLITGPTQGIGRVTALEVAKSGANMVLVARDSQ
                     KGQALVDEIKAAGNPNVELIVADLSVQSEVRRVASEFLARHETLHLLINNAGGIFHER
                     RETKDGLEMTFALNHLAYFLLTNLLLDVIKKSAPARIVNVASRAHTRGRIDFADLQKT
                     KSYAGWPTYSQSKLANVLFSHELARRLAGTGVTSNCLHPGVVATGFGRSDRGIWGLVT
                     RLAAPLLLSPEKGAATTIYLALSPEVETVSGKYFADCKVARPAPEALDLMVASRLWQV
                     SENLTGLAKDS"
     gene            complement(9271..>10235)
                     /locus_tag="PKL17_16900"
     CDS             complement(9271..>10235)
                     /locus_tag="PKL17_16900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK46463.1"
                     /translation="YSVGTLLNGLVDSVETYSLCRLLAGFGLAGELGAAVTLVSETLP
                     QSRRGLGTTLVAGFGLCGGVAAALLAEWLSWRTCYFVGGSAGLLLLFLRLSVAEPQMF
                     VDVKQQSVRRGSLALLLATATRRRRFLQLLLIGLPVWFVAGIVIAFSPELSTALGTQG
                     VTAARAVLISYIGVALGDLLCGVLSQVLKSRRRAMLWSLSLLLVLLLLLLRLHGLGLH
                     GFYALCFGLGLSTGYWAVLVTTAAEQFGTNLRATVTTTVPNLVRASVIPLGLLYQQLV
                     PTLGTVGSVRWLGGLCVAVAVVSLWLLPETYHRELAFLEHESRS"
BASE COUNT         2057 a         3304 c         2985 g         1889 t
ORIGIN      
        1 accggcaagc ggagggggcc tttggccgag aacaacaaac caggtggttc taacgttaac
       61 gacctcaggg ctcgtctcgg agtgaagaag gcgggcggtg gcccagctcc ggcaggtcca
      121 gggggtccag gggttcctgc gccagcaccc attccaggga tggcagcggg gcccggtcca
      181 ggcgcgcctc cgggtgcggt gcctgtgccg ccaggaatgg tcccgccacc agcgggcgtt
      241 ccggcgcctc cgggctttgc cgcaccggtt gagccgccga agcccgatcc atatgccgcc
      301 cagcaagccg ccatggctgc gtcgctggct gcgttttatg gtgcgggaca gtcgctgccc
      361 ggaaacgccg atgaagtcaa agacggcgtc ggcaagcagc gtccgctggg gctcatcatt
      421 ggcatcggtg tggccggtgt gctcggcgtt ctcgtcggct acacggtcaa cttcatcaaa
      481 ggccaaaacg agctgatcga gcttggtacc gagcgcgcca aggacatcaa gaaagaagtc
      541 gagtcggtcc gtggccgcgt caaagccgcg ctcgatgctt tcaaagagac caagccgggc
      601 gaggcaccgt cgaatgaaac gctcgacaag ctggcgaacc tcgatctgac cgaaccggat
      661 gcgacggcca agctgttctc gccgctgctc ggtacgttca acggcaagct cgtgcggtcg
      721 atgttcgact actacaacaa cgtgacggcg ttcttccgcg aggtgcagct ccatgcggca
      781 ctgacgaaga acgatcgcga tgtgctcgcc aagtgcatga aggcgggcgc cgaagctgcc
      841 gccaagggtc agccgccgct tggcgtcacc tttgattaca gccagaagct gccgcaagcg
      901 atcctggcta cgctcggcgg cgtggtctgc ccgaaagaag gcgagacgag ctgcgatccc
      961 ggcgaaatga agctgcgcta ccgcacatcg atgagcggcc agttccagga aaagccggcc
     1021 aaaggtctgc caaaggacat tgtgatcccg atccaaccga ccgagctgct caagaacgtc
     1081 gccgtcggtg atccgaatca gttggcctgc aaagactacg tccgtcgcat cgagcgcatc
     1141 cgtcagaccg tgaccaagat tcccgagctg gaaaagttcg tgatcgaagg tctcgacgat
     1201 cgcatcaaga acccgccgaa gtagttctgc aacaagcgct tcgctgcgct tgcttgtccg
     1261 tatccctcac tacatatttt tccacagcac actgcatcgt ggctttttgg cgcggtgcga
     1321 tcggctcgtt ttttggccac tgtggtccat cacgcgatgg cagcactggc atttttcgtc
     1381 cgcttttcga cggagccacc gctgcgtgcg ccagcccagc gcagtccggc aaatgacttc
     1441 gggaccgcct gcgctcaatt gatcacgtcg tcgttttcct gtattctgcg cagggaggtt
     1501 tagtctgagc caggttgagc aagcccggga ccctaggtcc ggaatggtga tcggcggcta
     1561 ccgtctgctc aagctgctgg gggccggagc gatgggccgc gtatacctgg ccgagcacga
     1621 gcgactggga cgccgcgctg cagtcaaggt tctcgacggc tgggacccag agctggtgtc
     1681 gcgacttttt tgcgaagccc gtgcggtggc tcagattggc gacgagcatc tggtcgacgt
     1741 ctatgacttc gttgccgatc cccaaaaagg cgtcgtggcg tatgtcatgg agtatctggc
     1801 cggcgaagac ctccgcaagc tgctccagcg cgagcgcgtg ctggccccaa gtcgagtcgc
     1861 ccgattggct gcacaaatct gcgaaggtct cgacgctgcc cacaaagttg gcgtcgtcca
     1921 ccgcgatctg aaaccagaca acatcgtcgt cgttcgtcgc ggcggcgaca gcgatttcat
     1981 caagatcgtc gattttggca tcgcccagtt tgccggcccc gtcgcccacc gcacaagagc
     2041 tggcatcgcc ctcggatcgg cggcgtacat ggcccccgag caagccgaag gaaagcgtgt
     2101 cgacggacga gccgatcttt acgccgtcgg tgtgctgatg ttcgagcttc tgacaggtga
     2161 agttccgttc cttggcaaga cttcacgcga gacgctggag atcatgcacg taaccaaagt
     2221 gccgtcgcca gtcggcgatc atggtgcggg cctggtcacc gacggtttgg cgaagctggt
     2281 ccagcgctgc atggccaagc gccccgaaga gcgattccag actgcggacg agctacgcaa
     2341 agcgctgctc gacatcgccg agattgcacc cgatcgcggc gacaaaacgc aggtgctcgg
     2401 tgcagtggcc aaacgcaagc ccgctgcctc gctgcgcagt ctgccgcccg cggcagtact
     2461 cggcatggcg attgcgtttg ccgttggact gggcgccggc ctggtcatca tgggcatcca
     2521 ctagcctcga aattccgacg gaaacagagc cacagatgcg cgtgctgcaa cttgacgcaa
     2581 ctggaaggtc caagcgatgg ttttgccgat gaagcggtct gttccttttg tctgggtcga
     2641 tgtgtttgcc gaagcgccgt ttggtggcaa cccgctcgcc gttctcgacg gaagtggcat
     2701 tcctgaagaa gcccacctgc cgctcgcacg ggagctaagc ctgtcggaaa cgacgttcta
     2761 tctaccgccg accacgcctg agtcagccga tctgcgggtc cgcatcttca cccaggcgaa
     2821 agaaattccc ttcgcgggcc atccgctttt gggaactgcc gtcgcggtgc tgctaaatgg
     2881 gaggctgccg tttcgtgccc cgcagacgac ggtgcgactg gaaaccggca tgggcgtggt
     2941 tcccgtcacg gtgaccggca gcgatctgcc cgagcgggcg cagttttcgc acgatcgcat
     3001 cagcgtccca ggaacgccta tcagcgacga acgacttgcg tggttgtctc cggcgttggg
     3061 gctgtcgatg gatcgcttcc gcacccgtgt ggagctgtcg tccgcatcag gcgaacgaat
     3121 tctctatccg caggtcgtca gcggcggcgc gcggcagctg atcgtaccga ttcagagcgt
     3181 cgaagaaatc gaagagctca gcgccagtta tcgcgatgtc gtcgtcgctg aaaaagcgct
     3241 tggggccgag ctgggcatcc tgtcgtttgc gttccgttcg cccccgcggg caagtgaacc
     3301 ggttcgggtg cgcgctcgct ttttcgctta cgatgcgcca aacgaagatc cagcgacggg
     3361 aagtgcggct gcatcgctcg cggtgtatct gcatcaccat cggctgctcg cacccggtca
     3421 ggcgctcgag atcgatcaag gtccgccgcg tggtcccatc gaaggggcac cgggtcgccg
     3481 atcgcttttg cgcgcgttct gtgacggaac gacggtgcag gtcgaaggcc gagtccgcga
     3541 agtgctgcaa ggcgacatgc ggctccagtt ttagcgtctg taccgcccta cccgtcgcag
     3601 ccctgtcgca acatctgacc cacgcgatac accacacgcc gacatttttt cccgccagct
     3661 tcgcggcttg ctgcatggca tccaggtgga gcctggcccg ctgctgtgtc agctcttttt
     3721 caaaaagcga cacgacatcg gacgggatgc gcgaatcggc atggagccgc aaagacagca
     3781 cggacaagtc atgcgccgcg cccagccgat ccgccagtcg atcgagcttg cgccgcaacc
     3841 gctcgtgcaa catctgttcg tcgcgcaggg ccgacagaat gcgcgcaagc tgccccattt
     3901 gggccgcaag cgagcgcgac ttccgcttgc cgatcacaaa ctgcgacagt cgcacaaggg
     3961 cccgcgctct tttgcacgct ttgcgggcac tgtgtaccaa gtctggcgaa tgctcgatgg
     4021 aagtcgaatc aaagagcgac tgcgccaccg caagctgctg gaccgcaatc gcttcgagcg
     4081 acggacccag tttttcgccc agccctgccg agagcgaaaa cgtcatggct ttgttttacg
     4141 gcaaaaacac ccgctgcaag aacgccgacg tcttgtcgac gaccagatcg ggaatctcgt
     4201 gaccgccacg gaacgcgaca aaatcgagct ttgcgccggc gccttgcagg tggtcgcgca
     4261 gccgctctgc ttgttcaaac ggcaaaatcg ggtccatgac gccgtggctt tgcaagatgg
     4321 gcagcccatg ggcaccgggc agccgcgaca gccactcctc ttcggcaatg atcgttcccg
     4381 accacagcaa aacgcccgcc agcttgcggc ccgcccgcag catcacgtcc gccgatagca
     4441 tcgcgccctg cgaaaaacca ccgagcaccg tcttgccttc cggcacccgc agcccttttt
     4501 ccagcaaatc gagcgtattg cagattttgt ctcgcgcgtc cgcgagcccg gctggaactt
     4561 cggcggccat tcgatcgaaa cgccgcgcca tcagatccat ctggattcgc tcgatgtcga
     4621 taagccacca cgcgcgcgag tccatcatgc caaagcccat ctcgggaatc gacgacagcg
     4681 cgatgggtgc cgccggaaat gcaaagcgcg tatcgcgcgg cgcccgcaaa tacggcgcaa
     4741 gccccacaag atccgtcccc ggcgcgccaa agccgtgcag cagcaccaca agcggacctt
     4801 cgccgccccc cattccatcg gtcccgcccg caatcaccac gtcgagctcg ccgattcgca
     4861 ctttttgcac ttttgccatc gtgtcttgtc cttttgccgc agcatcgcgc cgcgcctctc
     4921 ttttcgcaga ttttttccgc tgcgggtatc gccggttgca ctgtgcaatc gctgcgcaga
     4981 atctaatggc catgaaccac ttgctgcgag tcaccctgat gagtcttctt cccctggtcg
     5041 ccacatccgc cgaggccgcc gatccgaaag ccgcacccgc gtcgcagccc gccagcgaca
     5101 gcatccacag cctgtctgcc aagagcatcg acggcaaagc ggtatcgttt gccgattaca
     5161 aaggcaaagt cctgctcatt gtgaacaccg cgtcggagtg cggcttcacc gggcagtacg
     5221 ccggtctcga agcgctgtac gccaagtacc gcgaccgcgg cctggtcgtg ctgggattcc
     5281 cgtcgaatga cttccatcag gaaccagcca gcggtcagga aatcgccaag ttttgcaaag
     5341 ttcgctacgg cgtgacgttc ccgctgtttg aaaaagcgcc ggtttcgggt gcatctgcgt
     5401 cgccggtgtt caagtttttg gcccaaaaac acggcgagcc gaagtggaac ttctacaagt
     5461 atttggtggg aaaaaacggc caggtgctcg gtcagtattc gagccgaaca agccctgata
     5521 gcagtgatct gacagccgcg attgaagctg cgctgcgcta gctcgcgctc gacagatcgc
     5581 gtcgcaaaag acgcgcagtc agaagcatgc tgcgcccttg acagaccgcg agtcccttgc
     5641 gcgccagcgt cgtcaaacaa cgcgacaccg attcgcgggt ggcaccgacc atgttcgcca
     5701 gctcttgctg ggtcggacga cgccgcatca gcagtccacc ttggtgatcg gggctgcgct
     5761 cgtcgcgcgc caggcggatc aacatcttgc acagccgctc ttcgatgtcc tgcagcgcca
     5821 gcgcaaagat cgtttcttcg gcccggcgca tccgattggc catttccgac agaaggctga
     5881 tggtgagctg cggataacga ccgagcagcg ccaccacttc tgcgcgcgga agcgaaagga
     5941 cctgcacatc cgacagcgcc accaccgatg ctgtcgtcgg cgatccatcg agcagcgaag
     6001 tttccgcgat gagatcccct ggccgcagca tttccagcgt cacttcccgt cccgccgccc
     6061 cggtcatcac cactttggct gacccgctgg tgatcaccag cagtttgtct atcggcgtgc
     6121 tctgcggaac gatgatcaaa ccagcagtga tctgctccgg ctgcgacacc gccaaaagcc
     6181 gcgacagttc ttcatcgcta agcgccgcca atacccgcga tttccgaagc tgcgagatgt
     6241 cataagcaga caacaccgag tggcttgtgc aaccgcgctc aatctggact gcgatctcgt
     6301 tcattggact ccccgtgcac gctgcaatgc gagtgagccc aaaccaaagc aaagcctgcg
     6361 ccaacacgga ttggcccgtc gctcgtgaca aaccaggttc caccgtgcgg gcttgtcgtc
     6421 caaaagcggg cagaccgtgg tcatttttga ccaaacggaa ggatttacgc ggcggaactg
     6481 gtcgcttcgc gctggctact cgtcaaacga taggcttcgt ccatcagctc ggcgacgcct
     6541 tccccggtgg ctgccgagat cgcccagagt ttgacccctt gggccgcaaa ctgggcacgc
     6601 agcgtaggca gcgcatcgcg tgtttccgtc agatccagct tcgcgagcgt cacaagctgg
     6661 ggtcgttctg caagcccagg atcaaaccgc cgcagctcct cgttgagcgt cgcgtagtca
     6721 cgcagcggct cccgtcccgg ctcgtttgat acctcgagca gatgaatcag cacccgcgtg
     6781 cgctgcaagt ggcgcaaaaa gcgatgaccc aggccatgtc cttcgtgggc gccttcgata
     6841 aggcccggca agtccgcgag cacgaagctg cgatcgctca cccggctacc gagcggcagc
     6901 gacaccacac caagattcgg cgtcagcgtc gtaaacggat agttggcgat tttgggccgg
     6961 acccgcgaca cccgtgcaat cagcgacgat ttgccgacgt tgggatagcc aagcagacca
     7021 acatccgcga gaagctttag ctccagcttg agcgtccgct cttcacccgg tgttccaggt
     7081 tcagcaaact cgggtgcttg ccacgttgat gtcgcaaagt ggatgttgcc gcgaccgccc
     7141 tgcccgcctt gcgcggccac gaagcgctga cccggtgtcg ccaggtcgca cagaacattg
     7201 ccgtcgctgt ctttgaccac cacgccggca ggtacttcga cgatgagatc ctcaccgccg
     7261 cgaccatacc tgtcacgccc ttgtccggcc tggcctcgct cggcacggta ttcgcgccgg
     7321 tacgtcaaat caagcaaggt gctgcgctgg ggatctgcga tgagaatgac cgaaccgcca
     7381 ccgccgccgt cgccgcctgc tggaccgccg cgcggacggc ctggttcacg acgaaatgcc
     7441 gcacagccat cgccgccgtc gccgccacgg acaaagatcg aacactcgtc gataaactgc
     7501 atgggagttg ggcccggcca tcaactttgg accaggatgg ggccggattt tttgggagtt
     7561 cagaaaggga gaggagagac tagctcaccg acgcagcgcc agcatccgtc tgcacaggca
     7621 cgacggagac gcgcgaacgg ttgccacgcc aggtctcgaa cttcactttg ccgtcgatca
     7681 gcgcgaacag ggtgtaatcg cgaccgaggc cgacgttgcg acccgcgtga tagacggaac
     7741 cgacctggcg gaccaggatg ttgccagcgc tgaccacttc accgtcgaag cgcttgacgc
     7801 cgcgcatctt cggattggaa tcacgaccgt ttcggctact gccttgacct tttttatgag
     7861 ccatcgaagt ttcctgtaat caaacgtcga gcgtcccttt tttggacggg acgcgacgga
     7921 agctagcctc tgatgtcctc gatgcgcacc gccgtgtatt cctggcggtg accattcttg
     7981 cggcggtagc ccttgcgacg agcgtacttg aagacaatca gcttcttgcc tttttcgttg
     8041 ccctgcaccg aagccgtcac ttctgcggaa gcgaccgttg gctggccgac cttgacggtg
     8101 gtgccatcag cgacgagcag caccgggaag gtgatcttgt caccgctcgc ggcggcgatc
     8161 ttctcgacgc gaatgtagtc gcctttctga acccgatact gctttccccc ggcctcaatc
     8221 actgcgtaca tgttgtgaac cagctcctta cgcgagatgg caaatcagct tttgctgttg
     8281 gtttttgaat tgtccggcga cggcgttccg gatcgcctaa gccgtaaatg ccctgtgaaa
     8341 tcaaggcggt aagtgcgcag tctattccac ccgacgtcgc tgtcaagagt ctttggcaag
     8401 acctgtaagg ttctcgctga cttgccacag ccggctggcc accatcaagt cgagcgcttc
     8461 gggggccgga cgagcgactt tgcagtcggc aaaatacttt ccgctgactg tttcgacttc
     8521 cgggctcagc gccaaataaa tcgttgtggc cgcgcctttt tccggtgaaa gcaataaagg
     8581 tgcagccagc cgcgtgacca aaccccagat gccgcggtcg ctgcgaccaa aacctgtggc
     8641 gaccacgccg ggatgaaggc agttgctagt cacccccgtg ccggccagcc gccgcgccag
     8701 ctcgtgcgaa aacagcacat tggcgagctt ggactgactg taggtcggcc agcctgcata
     8761 cgacttggtc ttttgcaggt cggcaaagtc gatgcgaccg cgcgtgtgtg ctcgcgatgc
     8821 gacattgacg atgcgagccg gcgcgctttt tttgatcacg tcgagcagca ggttcgtgag
     8881 caaaaagtac gcgaggtggt tgagcgcaaa ggtcatctcc agcccgtctt tggtctcgcg
     8941 ccgctcgtga aaaatgccgc ccgcgttgtt tatcaaaaga tgcagcgttt cgtgccgcgc
     9001 caaaaattcg ctggcgacac gccggacttc cgactgcacc gatagatcgg cgacgatcag
     9061 ctcgacgttg ggattgcccg cagccttgat ttcatcgacg agcgcttgcc ctttttgcga
     9121 atcgcgggcg accagcacca tgttcgcacc gctcttcgcc acttcaagcg ccgtgacccg
     9181 cccgatgccc tgcgtaggcc cggtgataag gcaggtcttg ccgtgcagat ttggactatc
     9241 tcccatatcg aagacccttt ctgtaagccg ttaggatcgc gactcgtgtt ctaggaaggc
     9301 aagctcgcga tgatacgttt cgggcaaaag ccacagcgac accaccgcca cagcgacgca
     9361 aagaccgccg agccagcgca ccgaaccgac cgtcccgagc gtcggcacaa gctgctgata
     9421 caaaaggcca agcgggatca ccgacgcccg caccaagttc ggcaccgtcg ttgtcaccgt
     9481 cgcccgcaga ttggtgccaa actgctcggc tgccgttgtc accagcaccg cccaataacc
     9541 cgtcgacagg ccgagcccga aacacagcgc gtaaaaccca tgtaaaccca gtccatgcag
     9601 gcgcaaaagc agcaaaagca gcacgagcag cagcgacaac gaccacagca tggcgcgtcg
     9661 acggctcttg agcacctgag acaacacccc gcacaacagg tcgccgagcg cgacgccgat
     9721 gtacgaaatc agcacggcac gcgccgccgt cacaccctgc gtgccgagtg ccgtcgacag
     9781 ctcaggtgaa aacgcgatca caattccggc gacaaaccac accggcagac caatcagcaa
     9841 aagctgcaaa aaacgccgcc gccgcgtcgc cgtcgccagc aaaagcgcaa gactgccccg
     9901 ccgtaccgat tgctgcttca cgtccacaaa catctgcggt tctgcgacgg acaaacgaag
     9961 aaaaagcagc agcaaaccgg cgcttcctcc gacgaaatag cacgttcgcc aagacagcca
    10021 ttcggccaaa agcgccgccg caacaccacc gcacaaaccg aaaccagcga cgagcgtcgt
    10081 ccccaaaccg cgccgactct gcggcagtgt ctcgctcacc agcgtcaccg cagcgcccag
    10141 ctcgccggcc agaccaaacc ccgcgagcag tcgacaaagc gaatacgtct cgacggaatc
    10201 aaccagcccg ttgagcagcg taccgacgga ataga
//
LOCUS       DAORGN010000182         9884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2942_length_9884_cov_5.453556, whole genome shotgun sequence.
ACCESSION   DAORGN010000182 DAORGN010000000
VERSION     DAORGN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2942_length_9884_cov_5.453556"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKL17_16905"
     CDS             complement(<1..1078)
                     /locus_tag="PKL17_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46464.1"
                     /translation="MSFPKNRRRCQLTLSALLLSGAVLSGCASGEGTASGKDGVAAVP
                     ETPVALSSLGFDTARLNASKTPLFLSGRMDAAVVDGASAAQVLLARLAPSYRLSAETS
                     FQVLSEQTDENGLHYIRLGQLHAGIPVVDGEVVFQSERSGAVLAVIGELSPDLRVEVP
                     AAAKSPTAGTQAIRRALASVAKDGTLHFHGTSSLALVAPDTQPTDPVRLTHRALVEYV
                     GDEGMELAEVFVSVATGEVVSQRTHIHRALDRTLYTLGGNCIGTAMGMLPGTKVRKEG
                     DPTSTDTDANNVYDQQSSTYYFYKHLFNRDSYDDKGAGMIATVNVQFPAGFSCSPNNA
                     AWLGSPYFQMAYGKGDGMVSAECNT"
     gene            complement(1185..1955)
                     /locus_tag="PKL17_16910"
     CDS             complement(1185..1955)
                     /locus_tag="PKL17_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNK46465.1"
                     /translation="MLLRRIVSIIRLTVGFLFVSLCASLTILIALLLLPWRAARVKLC
                     NYYGKIVGRVILWLAGATPTVSHRERVEEFRPAIYVSNHASTLDAFVAIWICPIGGCG
                     VAKKEILRIPFFGWLYFLSGHLTIDRGDRSSAIAAMQKTAAFVLKNKLSIWIWPEGTR
                     SRDGRLQPLKKGFAHLALATGLPIVPVVVHRAHENWAKHGFTFVPIDLRIDVLPAIAT
                     AHWKSETLDEHIAEVHAAFAAQLGDSQKPLGPAISSGS"
     gene            complement(2002..3717)
                     /locus_tag="PKL17_16915"
     CDS             complement(2002..3717)
                     /locus_tag="PKL17_16915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46466.1"
                     /translation="MSESGVQRLGNYLLIERLGEGGMAEVYLAQYSGGEHSVGPASQV
                     VVKRIKPSLYKSTEFPVFREMFLNEAKLVRSLQHPNLARMYALLEAVDDELGVKVPFI
                     VGEYIRGTQLWELMRLATQGFTGKGVPPAIAAFVAREVARGLGHAHAHKDPGSGRPQP
                     IIHRDISPENIMVSQDGHVKVIDFGVAKALGGFGPQTRTGIIKGKLAYMAPEQVAQKV
                     VPATDVFGAGIVLHELLTGRRLFGGSNEYLVVSRVLKAEIPRPSSLLPGIPKELDDVT
                     MQALSRDLRGRFVDGNAMADALTRLLQNVPSLVGTTNQSIRDWAKQLTSEAKQLAQGW
                     EEQESGLHVDVQNALAHSVAKGEDIVELNPADLLVEGGQLDPGVKAAVTMGLKTLRPD
                     MLKSAQPSQPPKRPSSEGYKPPPLPGARPAANRVSTDALSAKVVPKPDPRRVTPVEPS
                     KSPVRALIPVVVPDPAASTVETVPAKLSSPEIPPLVASPNPSAGSIAGPDVAPSAALS
                     PPSPADATGTLDPVLPQKLAFLEPHLPPLIRSALRDDQVKRLALVISALLIVLLLLLF
                     AVVLR"
     gene            3920..4252
                     /locus_tag="PKL17_16920"
     CDS             3920..4252
                     /locus_tag="PKL17_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46467.1"
                     /translation="MKRTSILCLPALALLALTTMGCSTREKSICDKYITCEGGNDADK
                     SACVDNWIQRTQIAGAYNCGDAWSKYLDCMDASAICSSGKLKTDTCSAQNQAVATCVN
                     AASAYGVK"
     gene            4265..4663
                     /locus_tag="PKL17_16925"
     CDS             4265..4663
                     /locus_tag="PKL17_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46468.1"
                     /translation="MRIISTWRNAGIALALFTAVVAGCSGTEKSICDKGRQCLGGNDA
                     DYNACVQNYIYEGKIASDYKCSDAWSNYLTCLDSTSTCDSITVLGVTTKRLKNSCDAQ
                     ASALDSCEKAASTKGSEHFLTTEPASSTAK"
     gene            4784..7795
                     /locus_tag="PKL17_16930"
     CDS             4784..7795
                     /locus_tag="PKL17_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNK46469.1"
                     /translation="MFLLLLGLLPSTAHAGDPELLWRTIETEHFRITYHEPLGQVAQR
                     LAQVAETSHRVLVPVMKHSPRFRTEVLLTDDTDYSNGSATAMPFPTVRLYLTAPDDRS
                     ELNDFDDWLYALFVHEYTHVLHLDTMAGLPKWVNLLLGFGINNVYAPNQVQPRWFIEG
                     LAVLEETERSSAGRLRSAMFDMYLRSHVLEGKFLRLDQVSNQTRLWPRGNVPYLYGSA
                     FLRFVMKRFGHDSLAKVIARYGGCWSPDCWVPWGMSRSLKAVTGHTYGPLYEEFSQEL
                     KQRYADQLAALSQSPLGVTHPRPRSDWKTSVDRPLFVDGGKALLWLESDPYKRPALIR
                     FDLESGKATRELEVDGAGGLSVAPSGKFAVMARLNYFRANYSYQDLFLYRRDRRQLFA
                     LTSGLRVDHPAISPDGKRVAFETTSGGTRRLAVMDLPRNERPDESETINPKAFTVPEI
                     WRARAARPVSFPLQQKEWSQVYTPSWSPDGKILTFSYWQQGGFRDIVTLHLHSGELRY
                     ITHDRALDLEPRFSPDGKWIYFVSDRTGIYNLYAHHMESGVTYQVSNVVNGVFSPDIS
                     PDGKTAAVVGFVAEGYRIETLPIDLAQAPLAPAPPADRPEPLSVGDPELTRQKQHPVS
                     RYNPARTFFRVPASLLSAQLPISAPGPYGQSFGLQFQTSDIVGIHSLVGGLTIQSGRA
                     DATGVLARYSYKRLWNGINIDFSRSLYPRYGLRIGGKNQTYDEEAWQTGISTDLPLLR
                     DVARSATMYLAYNFTYWRNTSKVPIPSPDDISLRLPEVGRYGTLSLTLAYGDTRRFLF
                     SVGPERGGSIWVNASVAHPVLGSQYLVYNLRVAASQNIGIPWPSRYLRNHTLMLAYEG
                     GISGGDLKRRGVFYAGGFPQAEDFLRAALLGARPGQPRLRGYGAGETYGDQYHVLNIE
                     YRFPLLWLERGYDALPFYLWRLHGAVYSDVGGAFFGTISQDKIKASVGGELRLDGSLG
                     YYLPFMIQLGYAHGFMDGADNGFYFLLNNPL"
     gene            7815..8504
                     /gene="modB"
                     /locus_tag="PKL17_16935"
     CDS             7815..8504
                     /gene="modB"
                     /locus_tag="PKL17_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNK46470.1"
                     /translation="MRSPLALSFAVALLATMLSLSLGVLLASLLFQIGQPRPGRRFGI
                     QLAELGDAVLSLPLVLPPTVLGYYLLTILGRHSPLGRLYESLLGIPLTFTPQAAVVAA
                     MVSALPAIARSSRAALREVDPAYFRAAASLGASPWRALWTVGIPLCRRQIAAAAMLAF
                     ARALGDFGVTLMIAGDIPGYTQTASLAVYDAVQAGRESDALALIVPLSLFALVVMYVV
                     GRLQEPGAAKR"
     gene            8501..9208
                     /locus_tag="PKL17_16940"
     CDS             8501..9208
                     /locus_tag="PKL17_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNK46471.1"
                     /translation="MSEDAAQDKSVLIAALRTSPDGTSQLQIDLSLPVGVTVLIGPSG
                     SGKSTALDLIAGHRLPDFGHLSLSGQPLVRKESGLPPQLWIPPESRQIGYVMQQPMLF
                     PHLTVEQNLGYGIAAMPKKPRVDRIADIAAELDITSLLPRRPGELSGGQRQRVALGRA
                     LLPKPKALLLDEPLSAVDLGQRQKLLARLSALLLSLEIPVLYVTHSQEEQRFWNARTL
                     ALQASPRTPDLVETIQL"
     gene            9356..>9884
                     /locus_tag="PKL17_16945"
     CDS             9356..>9884
                     /locus_tag="PKL17_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46472.1"
                     /translation="MTITPDPKKTTGKQAPLGKPEPAVKPIEAVDSLDGQNHSNIEKI
                     RTILFGNQMRDYETRFVRLEEQLTKEVAELREDFRRRNDAIESFVRQELEALSERIAG
                     EYEGRAPSLKEISKSIDELSAGLDQKTHQLEEQLGKSNRELRQQVLTQSKQLRDDMQQ
                     GIAELTAVVSRELQQL"
BASE COUNT         2042 a         2969 c         2854 g         2019 t
ORIGIN      
        1 cggtgttgca ctcggccgac accatgccgt cgcccttgcc gtaggccatt tggaagtacg
       61 gcgaaccgag ccaagctgca ttgttcggcg agcagctgaa gcctgccggg aactgcacgt
      121 tcaccgtcgc gatcatgcct gcgcccttgt cgtcgtagga gtcgcggtta aataaatgct
      181 tgtagaagta ataggtgctc gactgctggt cgtagacgtt gttggcgtcg gtgtccgtcg
      241 acgttggatc gccttctttg cgaaccttgg tgccgggcag cattcccatc gcggtgccaa
      301 tgcagtttcc accgagggtg tagagcgtgc ggtccagcgc gcgatgaatg tgggtgcgct
      361 gcgaaacgac ttcgcctgtc gcaaccgaga cgaagacttc cgctagttcc atcccctcgt
      421 cgccgacata ctcgaccagt gcacggtggg tcagacgcac cggatcggtc ggctgggtgt
      481 ccggcgcaac caaagccagc gacgaagtac cgtggaaatg cagcgtgcca tctttggcca
      541 cactcgcaag agcgcgacgg atcgcttgcg taccagccgt cggcgacttg gccgccgcag
      601 gaacttccac gcgaagatcc ggcgaaagct cgccgatgac cgcaagcact gcgcccgagc
      661 gttccgactg aaacaccact tcaccgtcga caaccgggat gccggcgtgc aattgaccca
      721 gccggatgta gtgaagcccg ttttcgtcgg tttgttccga cagcacctgg aagctcgttt
      781 ccgccgacag acgatacgac ggcgcaagac gagcgagcag cacctgcgcg gcactggcac
      841 cgtcgacgac tgcggcatcc atgcgacccg acagaaacag cggcgtcttg ctggcattga
      901 gtcgcgcagt atcgaaaccg agagacgaca gcgcgacggg ggtttccgga actgcggcga
      961 ccccatcttt tccactcgcc gtcccttcgc cactcgcaca gccactcagg accgctccgg
     1021 acaacaaaag ggccgacagg gtcagctggc agcgacgacg attcttcgga aatgacatgc
     1081 aagactcctt ggttcaggca acgtgcagac gtttttggtt tgggaaccta tctgccggaa
     1141 cctataccac aaggaagcga tgtcgagccg tcgttgacgt gcgatcaact acccgacgaa
     1201 atggccggac caagcggctt ttggctgtcc ccaagctgcg cggcaaacgc ggcatgcact
     1261 tccgcaatgt gctcgtcgag cgtttcggac ttccagtgcg cggtcgcgat cgccggcagc
     1321 acgtcaatcc gcagatcgat cggcacaaac gtaaagccat gcttggccca gttttcgtgg
     1381 gcacgatgca cgacgacggg aactatcggc agccccgtcg ccagcgccag gtgagcaaag
     1441 ccttttttca gcggctggag tctgccgtcg cggctgcgcg ttccctcggg ccagatccaa
     1501 atgctgagct tgtttttgag cacaaacgcc gcggtctttt gcatcgccgc aatcgctgac
     1561 gagcgatccc cgcgatcgat cgtcagatgc cccgacaaaa agtagagcca gccgaaaaaa
     1621 ggaatccgca ggatctcttt tttggcgacg ccgcagccac cgatcgggca aatccagatc
     1681 gcgacaaagg cgtccagcgt cgaggcgtgg tttgacacat agatcgcggg acggaactct
     1741 tcgacccgtt cacggtgtga aactgtcgga gtcgccccag ccaaccacaa gatcacccgt
     1801 ccgacgatct tgccgtagta gttgcagagc ttgacccgtg cggcccgcca cggcaaaagc
     1861 agcagcgcga taaggattgt cagcgacgca cagagcgaca caaagaggaa accgacggta
     1921 agccggatga tcgagacgat tcgacgtaga agcacagccg cgcagtatga ctctgagtgg
     1981 gtgtcttcgt ctacgaattc atcagcgcag cacgacggca aacaaaagca gcaacagcac
     2041 gatgagaagt gcgctaatca ccagcgccaa ccgcttgacc tgatcgtcgc gcagcgccga
     2101 gcgaatcaac ggcggcaggt gcggttccaa aaacgcaagc ttctggggca gcacggggtc
     2161 gagcgttcca gttgcatcgg caggcgacgg tggagacagc gcagccgacg gcgcaacgtc
     2221 tgggcctgcg atcgatccgg ctgacggatt tggggacgcc accagcggtg gaatttccgg
     2281 ggacgacagc ttggcaggaa cggtttcaac ggtcgacgcc gcggggtcgg gaacgaccac
     2341 tggaatcagc gctcggactg gcgatttgct cggctcgacg ggagtgaccc gacgcggatc
     2401 tggctttgga acgactttgg ccgacagcgc atcggtgctg actcgattgg ccgcaggtcg
     2461 cgcccccggc agaggcggtg gcttgtagcc ttccgacgac ggacgcttgg gcggctgact
     2521 gggttgggcg gacttcagca tgtcgggacg cagcgtcttt agacccatcg tcaccgcggc
     2581 tttgacccca ggatcgagct gcccaccttc gacgagcaga tccgcaggat tgagttcgac
     2641 gatgtcttcg cctttggcca cgctatgcgc cagcgcgttc tgcacatcga cgtggagccc
     2701 gctttcctgt tcttcccaac cctgcgcaag ctgcttggct tcacttgtaa gctgcttggc
     2761 ccagtcacga atggactgat tggtcgtccc cacaagcgac ggcacgttct gcaaaagccg
     2821 cgtcagcgca tccgccatcg cattgccgtc gacaaaacgc ccacgcagat cgcgactgag
     2881 cgcctgcatc gtcacgtcgt ccagctcttt gggaatgcct ggcagaaggc tgctgggacg
     2941 aggaatttcg gcttttagca cccggctgac gaccaaatat tcgtttgatc cgccgaacag
     3001 ccgtcgcccg gtcaatagtt cgtgcagcac aattcccgcg ccgaacacat cggtggccgg
     3061 gacgactttc tgcgccactt gctcgggcgc catgtacgca agcttgcctt tgatgattcc
     3121 ggtgcgcgtc tgcggaccga aaccacccag cgctttggcg acgccgaagt cgatgacttt
     3181 tacatgtcca tcctgcgaca ccatgatgtt ttcaggtgaa atgtcgcgat ggatgatcgg
     3241 ctgcggtctt cccgatccgg gatctttgtg ggcgtgcgcg tgtcccaaac cccgtgcgac
     3301 ttcacgcgcg acaaacgccg caatcgccgg aggcacgcct ttgccggtaa agccctgggt
     3361 ggcgagccgc atcagttccc aaagctgggt tccgcgaatg tactcaccaa cgataaatgg
     3421 aacttttaca cccagttcat catcgaccgc ttccagcagt gcatacatcc gcgccaggtt
     3481 cggatgctga agcgagcgca ccagcttggc ttcgtttagg aacatctcgc ggaagaccgg
     3541 aaactccgtg ctcttgtaga gcgacggctt gatccgcttg acgacgacct ggcttgccgg
     3601 tccaacggaa tgctcaccgc cggaatactg ggcgaggtag acctcggcca ttccgccttc
     3661 ccccaagcgc tcgatgagca agtagttgcc cagccgctgg acgccagact cggacatcac
     3721 ttcctaaagt aacgcagttt tcggagattt ttcaccgact ttcgcgcccc catcgtcacc
     3781 tacatccgag gcagcacgcg acgccggggc caccataccg acgccaaaac caacgcttgg
     3841 gcagatcatg tcaggtgaag gaccgttgac cggaccgcca cccctgtttc aagataggaa
     3901 aaccgaaagg agagagctta tgaagagaac cagcatcctt tgcctaccgg ccttggcgct
     3961 cctcgccttg accaccatgg gctgtagcac gcgcgaaaag tcgatctgcg acaagtacat
     4021 cacctgcgag ggcggcaacg acgcagacaa gagtgcttgc gtggacaact ggatccagcg
     4081 cacccaaatc gccggcgcct acaactgcgg cgacgcctgg tcgaagtacc tggactgcat
     4141 ggacgcatcg gcgatttgca gcagcggcaa gctgaagacc gacacctgct cggcccagaa
     4201 tcaggctgtg gcgacctgtg tcaacgcggc cagcgcctac ggcgtgaagt aaaagaggac
     4261 taccatgcgc atcatttcta catggcgtaa cgcaggaatt gctctggccc tgttcaccgc
     4321 ggtcgtggcg ggctgctcgg ggaccgaaaa gtcgatctgt gacaagggcc gtcagtgcct
     4381 cggcggcaat gacgccgact acaacgcctg cgtccagaac tacatttacg aaggcaagat
     4441 cgcgtcggac tataagtgtt cggatgcgtg gagcaactac ctgacctgcc tcgacagcac
     4501 cagcacctgt gattcgatca ccgtgcttgg cgttaccacc aagcggctga agaacagctg
     4561 cgatgcccag gcgtcggcgc tcgatagctg cgaaaaagcg gcatcgacca agggttcaga
     4621 gcactttctg accaccgagc cggcgtcctc gaccgcaaag taacgtcccc gcctccgaat
     4681 ccgccggctt ttctgctatg accttcttgg tctgatggtc attttctccc gctcccaaag
     4741 gaattctcgc tttcccatgt tgtgggaggg attcctgtgc gccgtgtttc tcctgctgct
     4801 tggtcttctg ccgtctacgg cgcatgcggg agatccggaa cttctgtggc gcacgatcga
     4861 aacagagcac tttcgcatca cctatcacga accgctcgga caggttgcac agcgtctggc
     4921 gcaggtggcc gagacatccc atcgggtgct ggttccggtg atgaaacaca gcccgcgctt
     4981 tcggaccgaa gtgctgctga ccgatgacac agactactcc aatggatcgg cgaccgcgat
     5041 gccgtttccg acggtgcggc tctatctaac agcgcccgat gatcgatcgg aactcaacga
     5101 ttttgatgac tggctgtacg cactgtttgt ccacgaatac acccacgtcc tgcacctgga
     5161 tacgatggcc ggactgccca agtgggtgaa cctgctgctc ggttttggca tcaacaacgt
     5221 ctacgcgcca aaccaagtgc agccgcgctg gtttatcgaa ggactcgcgg ttctcgaaga
     5281 aacagagcgc agcagcgccg gacgactgcg atcggcgatg ttcgacatgt acctgcggag
     5341 ccatgtgctg gaaggaaagt tcctgcggct cgatcaggtt tccaatcaga cgcgactgtg
     5401 gccgcgtggc aacgttccgt atctgtacgg atcagcgttt ctgcgctttg tcatgaagcg
     5461 atttggtcac gactcgctcg ccaaagtcat cgcccggtac ggaggctgct ggtccccgga
     5521 ctgctgggtt ccatggggaa tgagtcggtc gctcaaagcc gtgaccggac atacgtatgg
     5581 accgctctac gaagagtttt cacaagagct aaaacagcgc tacgcagacc agcttgcagc
     5641 gctgtcccaa tcgccactgg gagtcaccca tccacgcccc cgatcggact ggaaaacaag
     5701 tgtcgatcga ccgctgtttg tcgatggtgg gaaagcactt ctgtggctgg aaagtgatcc
     5761 gtacaaaaga ccagcgctga ttcgttttga tctggaaagc ggcaaagcca cccgcgagct
     5821 ggaagtcgat ggagccggag gtctgtcggt tgcccccagc ggcaagtttg cagtgatggc
     5881 gcggctcaat tatttccgcg ccaattacag ctaccaagac ctgtttctgt atcggcgtga
     5941 ccgacgacaa cttttcgcac tcacttcagg actgcgcgtc gatcatccag cgatttctcc
     6001 ggatggaaaa cgggttgcat ttgaaaccac aagtggcgga acgcgcaggc ttgcggtgat
     6061 ggatctgccg cgcaatgaac gtccagatga aagcgaaacc atcaatccca aagcgttcac
     6121 ggttcccgaa atttggcgtg ccagagccgc ccgtccggtg tcgtttccac tgcaacaaaa
     6181 ggagtggtcg caggtctaca cgccaagctg gtcccccgac ggaaaaatac tgaccttttc
     6241 ctattggcag cagggcggtt tccgcgacat tgtgacgctc catttgcaca gtggcgagct
     6301 gcgctacatc acccacgatc gcgcgcttga tctggagcca cgcttttccc ccgatggaaa
     6361 gtggatctac tttgtttccg atcgaaccgg aatttacaac ttatacgcgc atcatatgga
     6421 aagcggagtc acctaccagg tctccaatgt tgtaaacgga gtcttttccc cggacatctc
     6481 tcccgacggg aaaaccgcag cggtagtggg ctttgtggca gaaggatatc gcatcgaaac
     6541 cctgcccatt gatttggccc aagcaccgct tgcccctgcc ccacccgcgg atcgtccgga
     6601 accgctcagc gtgggcgatc cagagcttac ccgtcagaaa caacatccgg tttcgcgata
     6661 caatccggcg cgcacgtttt tccgcgtccc ggcgtcgctg ctctcggcgc agcttcccat
     6721 ttcagcccct ggtccctacg gacagagctt cggtcttcag tttcagacca gcgacatcgt
     6781 cggcattcac agcttggtcg gcggactcac gattcagtca ggacgcgccg acgccaccgg
     6841 agtgctcgcg cgatatagct acaagcgact gtggaacgga atcaacatcg actttagtcg
     6901 cagtctgtat ccccgctacg gcctgcggat cggcggaaaa aaccaaacct acgatgaaga
     6961 agcgtggcaa accggaatca gtaccgatct gccgcttctg cgtgatgttg cacgctcagc
     7021 gacgatgtat ctggcataca acttcaccta ctggcgcaac acatcgaaag ttccgattcc
     7081 cagtccggat gacatttcgc tgcggcttcc ggaagtcggt cgttatggca cgctgagttt
     7141 gacgctggcc tacggagaca cccgtcgctt tctgttttcc gtcggaccag agcgcggtgg
     7201 ttcgatctgg gtgaatgctt cggtggcgca tccggtgctc ggttcccagt atttggtgta
     7261 caacctgcgt gtcgcggcgt cgcagaacat cgggattccg tggccatcgc gctaccttcg
     7321 caatcacacg ctgatgctgg cgtatgaagg aggaatcagt ggtggggatc tgaaacgacg
     7381 cggcgtgttt tatgcgggcg gttttcccca agccgaagac ttcttgcgcg cagcactgct
     7441 cggggcgcgt cccggtcaac cgaggctgcg tggctatggc gcaggagaga cctacggcga
     7501 tcagtatcac gtcctaaaca tcgaataccg cttcccactt ctgtggctgg aacgcggcta
     7561 cgacgcgctg ccgttttatc tgtggcgctt gcacggtgcg gtgtattccg atgttggtgg
     7621 cgcgtttttt ggcaccataa gccaagacaa gatcaaggca tccgttggtg gagagctgcg
     7681 tctcgatggc tcgctcggat actatctgcc gtttatgatt cagcttggtt atgcgcacgg
     7741 cttcatggat ggcgcggaca acggcttcta cttcctgcta aacaatccgc tctgagtcgg
     7801 tatagtggcc cgccatgcgc tcaccccttg cgctttcgtt tgctgttgca ctgctggcga
     7861 ccatgctgtc gctttcgctg ggtgtccttt tggcgagtct gctgtttcaa atcgggcagc
     7921 cgcgtccagg tcgccgcttc ggtatccaac tggcggaact tggcgatgcg gttctgtcgc
     7981 ttccgcttgt gcttccaccg acggtgcttg gctattattt gctgacgatt ctgggtcgcc
     8041 acagtccgct tggacggctg tatgaatccc tgctagggat tccgctgacg ttcacgccgc
     8101 aagctgcggt cgtcgctgcc atggtgtctg ctctgccggc aatcgcccgc agcagccgcg
     8161 ccgcactgcg cgaagtggac cctgcttact ttcgcgctgc cgcatccctt ggtgcaagtc
     8221 cgtggcgagc gctgtggact gtaggaatcc cactttgccg tcgacaaatt gctgccgctg
     8281 caatgcttgc gtttgcacgc gcgctggggg attttggtgt caccctgatg atcgccggag
     8341 acattccggg gtacacccaa acggcctcct tggcggtcta tgacgcagtg caagccggaa
     8401 gggaatccga tgcactggcg ctgattgtcc ctttgtctct gtttgcgctt gtcgtgatgt
     8461 atgtcgtggg tcgattgcag gaacccggag ccgccaagcg atgagcgaag acgcagcaca
     8521 ggacaaaagc gtacttatcg cagcgctgcg aacatcccct gacggcacgt cacagcttca
     8581 gatcgatctc tcgctgcccg tcggtgttac tgtgctcatc gggccctctg gttcgggcaa
     8641 aagcaccgcg cttgatctga ttgccggaca caggcttccg gatttcggtc acttgtccct
     8701 gtcaggacag ccgcttgtgc gcaaagaatc cggactcccg ccgcagctgt ggattccgcc
     8761 cgaatcgcgt cagattggct acgtgatgca gcagccgatg ctgtttccgc atctcacggt
     8821 cgaacagaat ctggggtatg gaatcgctgc aatgcccaaa aagccgcgcg tcgatcgaat
     8881 tgccgacatc gccgccgagc tggacatcac atctcttctg ccacgccgcc ctggcgagct
     8941 atcgggtggt cagcgtcagc gagttgcact ggggcgagcg cttctgccaa agcccaaagc
     9001 gctgcttttg gacgaacccc tgtcggcggt ggacttgggc cagcggcaaa agctccttgc
     9061 gcgcttgtct gcgctgcttt tgtctcttga aattcccgtg ctttacgtta cgcacagcca
     9121 agaagagcag cgcttctgga atgcccgaac cctggcgctg caagcctcgc cgcgcacgcc
     9181 ggatctcgtc gaaaccatcc aactgtaaaa cgactgtcga gtgaccactt gtacccacag
     9241 ggtcggtgcc gagtgaagcc aatcggcgca gtgcgtccgt cagtaaactt gcatttttat
     9301 agtgattttt tacggacttg gtgaataatg tgttcaaaat tacaaaaggt ttgtcatgac
     9361 gatcacgccc gatcccaaga agaccaccgg aaaacaggcc ccgctgggca agcccgagcc
     9421 agcagtgaag ccgattgaag cagtcgattc cctggacgga cagaatcaca gcaacatcga
     9481 aaaaattcgc accattttgt ttggcaatca gatgcgcgat tacgagacgc gctttgtccg
     9541 tctcgaagag caactgacca aagaagtcgc cgagctgcgg gaagatttcc gtcgccgcaa
     9601 cgatgcgatc gaaagttttg tgcggcagga actcgaagca ctcagcgagc gaatcgctgg
     9661 tgagtatgaa ggccgcgccc catcgctcaa ggagatcagc aagtcgattg atgagctgag
     9721 cgctgggctc gatcaaaaga cgcaccagct cgaagagcag cttggcaagt caaaccgcga
     9781 gctgcgtcag caggtgctca cgcagagcaa gcagcttcgc gatgacatgc agcaaggtat
     9841 cgccgagctt acggcggtcg tatcgcgaga gctacagcag ctcc
//
LOCUS       DAORGN010000183         9831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2973_length_9831_cov_5.660291, whole genome shotgun sequence.
ACCESSION   DAORGN010000183 DAORGN010000000
VERSION     DAORGN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2973_length_9831_cov_5.660291"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PKL17_16950"
     CDS             complement(<1..1356)
                     /locus_tag="PKL17_16950"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HNK46473.1"
                     /translation="MTTPSAGQNPAPLSFSDSPQSWLTPGERPKTIVDSLLSRPDATR
                     LLRATPIQPLYLLIKSLGLADAHELIAMCLPEQIQGLIDLDVWDRDELKSGQLGAWLS
                     HLVELPPQRCASHVRKLDPELLTTFLANQVRVYDLSEEDLVPDEPQGMFYETPDRFFV
                     IDILPSQDGDSDRAVLIHRLLEHLYRGDLDLARAVVNAARWDSGAETEELAYRFRSGR
                     IADLGFVEYYDALRIYQEVDPKAKPPAKTTAATHKHPAQHAAGDVKGTGPGGFLDAES
                     GLWVQLVPQLGQPSSLLYRVTSGLDESERNDLLQELVYLGNQAMSADRVPMDDLAQTE
                     QTLQRTAGYLLLGLAQLCGQKGAQAGSAHESAVAIDMLRSVPLAYLFRLGHSLTQHVR
                     KLAKLLVDGGATTVDKTLSPTSLLPKPEADALLAILAPRPLYPVSLDDPQKSGARPFA
                     SL"
     gene            complement(1372..2085)
                     /locus_tag="PKL17_16955"
     CDS             complement(1372..2085)
                     /locus_tag="PKL17_16955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HNK46474.1"
                     /translation="MNSASESAQPDVIAIRFRLQYPDVDTFVAKYAVNISEGGIFIAS
                     MDPPAVGSIVRFELSIAGGQPILRGEGQVVWNAPFDPLRPMDTCGMSLRFLRMDADGV
                     ALIRRVLSYKSDHPERFFTAAPDPYATAAYRPQPPAQSHPQVAQTPQPSVSQDPSSKQ
                     TPVHDSLAASQLSMSARTPVPGEAPRPASHPSMITPAALEEELADLLKPKAASTVTPA
                     DASKKLSELLDRRLRPPRT"
     gene            2239..3642
                     /locus_tag="PKL17_16960"
     CDS             2239..3642
                     /locus_tag="PKL17_16960"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNK46475.1"
                     /translation="MRRIVSWALAMLFAGVGCHSGRPDYTVNVPPNLVDIRPPRTVVF
                     VQAPLRLSTLEAAVDRQLPGGSQGNVNLGLLQLGWRLGRQPATVVPAGDGLSIRIPVV
                     GDLSLGGGFLRCQSSGVGGALVISARPTIEGGGDLVLRDIRTSVEPVGQIQCGGLPLP
                     VADVFSAMLRPLQSLAQQLGQLVKIPLGPVLAQGIAELSTPRPLAMAGRKTCLDLHPS
                     ALVLAPPSAAPDGSHAISVRIGLDVEPRLSLGDCPSGGGRTPASLTVRQENLGEEFAI
                     QVAVAVPMSDLKSMLGPQLVGKRLGSAAQSLLVQGVEVGDANGRVLLRLDVAGIYTGA
                     LFLWGTPKLVSEGGRQILQVPDLQVAAESSSKLQELKVAAYELVEGNLADKVRPHLRL
                     DVTDKLAESQRALSGTIKLQGGAWQNVQPAAQALGISQVALQTDLSQVLPLAVEAKPG
                     VVVAYVLLRGRSLLSIQ"
     gene            3711..3941
                     /locus_tag="PKL17_16965"
     CDS             3711..3941
                     /locus_tag="PKL17_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46476.1"
                     /translation="MKSKTKKLTGTVHHVDLEGGYFTLRLAGGEIYKLDGGGADLRKA
                     GVKAEVEGEIEDKGFGISFGTPTVKVKSYKVL"
     gene            complement(4011..5171)
                     /locus_tag="PKL17_16970"
     CDS             complement(4011..5171)
                     /locus_tag="PKL17_16970"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNK46477.1"
                     /translation="MGKRALIIGAGIGGLCTAIALRKVGYTVRIFERTQEVRALGAGM
                     SLWSNAMWALHVLGLSDEVLRIGSVVDITRTQTAEGELLSDVHIGEISRRLGVPSVCI
                     HRADFHQILVNAVGAANIQTGMQCTGVAQDEATVTAIFENGELHRGDLVVAADGFQSA
                     IRKQLVGADEPRYSGYTCYRSVVSMVHRTLPPGLALTAIGHGTEFGLFPCGMGRIYWF
                     ATHNQPAQKTDSPRGRKADCLTTFAGFPEPFVHALHQTPEADILRHDVYDRAPLLHFG
                     DGRVTLLGDACHPMTPNLGQGACMAIEDAVVLARCLRDEPNEVKALRHYETARRERTT
                     LMTTRSYYAGKVLTLEDGVLSWFRNRLLHSKLGGTQAESELEKLLSYQVPTL"
     gene            complement(5188..5691)
                     /locus_tag="PKL17_16975"
     CDS             complement(5188..5691)
                     /locus_tag="PKL17_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46478.1"
                     /translation="MNRASLKLCGTLVLAALVCDSRPARAGGDIIVLPGQQMAFILEG
                     AMATCSLTSIVGNAVTGVRKTPSRAWMYSGFICGFINTATSPIVMIYGTDPKVEYGIG
                     MGLMHGVVGLTNLGLSIWNAHMWHKARTAAPEAPPPAAPAVSLAPMVGRDKQGANILG
                     LSLSASY"
     gene            complement(5688..6809)
                     /locus_tag="PKL17_16980"
     CDS             complement(5688..6809)
                     /locus_tag="PKL17_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46479.1"
                     /translation="MLTRGVFATLLVVGGLFAIASQAVAQTKTLRATAGPFGTRGKHC
                     NDEQKRKLMLEHFMGGSINPLGIENQLILTYCLPFVEKPGILWDYTKLEFGLANYISP
                     THFHIGPTVRFAPLSFLILRAELTGFYIWPIPLQGAGYIEVQGYDDFKAETVKPALGV
                     AKDMFGLRTALGVTLQGALPLGKWVDILIVNGLTGEFWRAANGDFASPFWYITKLDGI
                     MDGRGDWALVNNAALIVSIKPHKNHAIRIGATDNLLYIPGSGYKGNIAAGILAWSVSN
                     LRDLAKSFAFFMRVGTFTHHAFRSGITLAAGLDVTYELSSTPTRRAEEEQQAAPTDAA
                     TSATTTLSETPAAPAAQQSPTASPAPADATTTPAPEGQK"
     gene            7107..8057
                     /gene="mdh"
                     /locus_tag="PKL17_16985"
     CDS             7107..8057
                     /gene="mdh"
                     /locus_tag="PKL17_16985"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNK46480.1"
                     /translation="MHHTTSGRKKIAVIGGGNIGGELAALIARKELADVVLLDIPEKD
                     AFCKGKALDLMQNGTLLGWDCNITGTGNWADAAGANVVIITAGVPRKPGMSRDDLLSV
                     NLKIMRSVAGNIKTHCPDSFVIVISNPLDAMVYECAKVTGFPKNRICGMAGVLDSARF
                     QLFLAQEMGVSVKDVKAMVLGGHGDTMVPVTSYCTVNGIPVKQFISPERLAAICARTA
                     GGGGEIVKLMGTSAYYAPATAAVVMAESFLHDQKRLVPAAVYCNGEYGISDLYIGLPC
                     VIGKGGVEKVLEITLTDDEKAQLGKSVAAVRELVDASVRL"
     gene            8165..9358
                     /gene="sucC"
                     /locus_tag="PKL17_16990"
     CDS             8165..9358
                     /gene="sucC"
                     /locus_tag="PKL17_16990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNK46481.1"
                     /translation="MNIHEYQAKELLRKKGLPVPVGKSALSVDDADSAAAWVRSETGN
                     PIVVVKAQIHAGGRGKGGGVKVTKTAEDTQAQIRKIFGMQLVTHQTGPEGKKVGRLLL
                     EQGVDIAKEFYAAVVLDRQKGRLVLMVSTEGGMDIEEVAAHQPEKIFKAVIDPMTGFT
                     PYQAREVAFALGLKSAGMEVVNSACKFFATMVRAYQELDCSLLEINPLVLTKQGQIIA
                     LDAKINFDDNALYRHPDIEEMRDLEEEEPAETEAKKFDLSYVKLTGNIGCMVNGAGLA
                     MSTMDIIRFYGGMPANFLDVGGGATAEKVTAAFKIITADPAVRGIFINIFGGIMKCDT
                     IAKGVITAVKEVGLKVPLVVRLEGTNVELGKQILAESGLNVVAASDMADGAQKIVALA
                     GESAA"
     gene            9369..>9831
                     /locus_tag="PKL17_16995"
     CDS             9369..>9831
                     /locus_tag="PKL17_16995"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812253.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNK46482.1"
                     /translation="MSILVNKNTRLLVQGLTGKNGTFHTEKAVEYGTTVVGGVTPGKG
                     GSTHLNLPVFDTVADAVKQTGANASVIYVPAAIAADSILEAIAAGLELVVCITEGIPV
                     MDMLKVRRVLDAQTGQKRTRLIGPNCPGIITPGECKIGIMPGYIHKPGHIGV"
BASE COUNT         1896 a         2860 c         3193 g         1882 t
ORIGIN      
        1 caggctggca aacggtcggg caccgctctt ttggggatca tccaggctga cgggatacag
       61 aggccgcggc gccaagatcg caagcagcgc gtcggcttct ggtttgggca aaagcgatgt
      121 cggggacagc gttttgtcca ccgtcgttgc tccgccgtcg acgagaagct ttgccagctt
      181 gcgcacatgc tgcgtcagcg aatgcccgag ccggaacaga taggccagcg gcacgctgcg
      241 caacatgtca atcgccactg cggattcgtg ggcgctgccg gcttgggcac ctttttgacc
      301 acacagctgc gcaagcccca acagcagata gcctgcggtg cgctgaagcg tctgttccgt
      361 ctgcgccaag tcatccatcg gcacccggtc ggcgctcatc gcttggttgc ccagatagac
      421 cagctcttgc agcagatcgt tgcgttcgct ttcgtcaagc ccgcttgtca cccggtagag
      481 cagcgacgaa ggctgaccga gctgtggcac aagctgcacc cacagaccgc tttccgcatc
      541 gaggaagcca cctggccccg ttccctttac atcgccggct gcgtgctgtg cgggatgctt
      601 gtgcgtcgct gcggtggtct tggctggcgg cttggctttc ggatcgactt cctgataaat
      661 gcgcagtgcg tcgtagtatt cgacaaagcc cagatccgcg atgcgacctg atcggaagcg
      721 ataggccagc tcctcggttt cggcgccgga atcccagcgc gcagcgttta ccaccgcacg
      781 ggccaagtcg agatcgccgc gatacaggtg ttcaagcagc cgatggatca gcaccgcgcg
      841 atcgctgtcg ccgtcttgtg acggcagaat gtcgatcaca aaaaagcgat cgggcgtttc
      901 gtaaaacatg ccctgtggct cgtcgggaac cagatcctct tcgctcaggt cgtagacgcg
      961 cacctggttt gccaaaaacg tcgtcaaaag ttccggatcg agcttgcgga cgtgggatgc
     1021 gcagcgctgc ggcggtagct ccaccaaatg cgaaagccac gcgccaagct gcccagactt
     1081 tagctcgtcg cgatcccaga catccaagtc gatgagcccc tggatttgtt ccggcaggca
     1141 catcgcaatc agttcgtgcg cgtcggcaag tcccagcgac ttgatcagca aatacagcgg
     1201 ctgaatcggg gttgcccgca aaagccgcgt cgcatcgggt cgtgaaagca agctgtcgac
     1261 gatggttttg ggccgctcac caggcgtaag ccagctctgg ggtgagtcgg aaaaggaaag
     1321 cggagccggg ttttgcccag cacttggcgt cgtcatggcg gcgacgctat gtcaagttcg
     1381 gggtgggcgc aagcgacggt cgagcagttc tgacagcttc ttgctcgcgt cggctggcgt
     1441 gaccgtggac gccgccttcg gtttcagcag gtcagccagt tcttcttcga gcgccgctgg
     1501 ggtaatcatc gacggatgcg aagcagggcg gggcgcttca ccgggcaccg gggtgcgcgc
     1561 ggacatcgac agctgcgatg ccgccagcga gtcatgcacc ggcgtttgtt tcgaggacgg
     1621 atcttggctt accgaaggct gcggcgtctg ggccacctgg ggatgtgatt gcgctggtgg
     1681 ctgcggacga tacgccgccg tcgcgtaggg atcgggcgcc gcggtgaaaa aacgctcggg
     1741 atgatccgat ttgtagctga ggacgcggcg aatcagcgca actccgtcgg cgtccatccg
     1801 cagaaagcgg aggctcatgc cgcaggtatc catcggacgc agcggatcaa atggcgcatt
     1861 ccacaccacc tgaccttcac cccgcaggat gggctgaccg ccggcaatcg acagctcgaa
     1921 acggacaatc gatccaactg ctggcgggtc catcgacgcg atgaagatgc cgccttccga
     1981 aatgttgacg gcgtacttgg caacgaacgt gtcgacatcg ggatattgca agcggaatcg
     2041 gatggcgatc acgtcgggct gcgccgactc gctggcagag ttcacgagcg ccagtatttg
     2101 ttcccatcgc cccggctgtc aattgtacag gcgaccttgc gacgacctgg gctcgtcaca
     2161 aagacggcga catcgcacag cacgggtcgg tgaagtgcaa gcgggcgccg cgggtctggc
     2221 gtagtagact ccttggcgat gcgcaggatt gtttcttggg cgctggcgat gctgtttgct
     2281 ggcgtaggtt gtcactccgg tcgacccgat tacaccgtca acgtgccgcc gaacctggtc
     2341 gacattcggc ccccgcggac ggtggtgttt gtgcaagccc cgctgcggct atcgacgctg
     2401 gaagcggcgg tcgataggca gttacccggc ggcagccagg gcaacgtgaa cttggggctt
     2461 ttgcagctgg gctggcggct cggtcggcag cctgcgacgg tggttccagc cggcgatggt
     2521 ctttcgatcc ggattccggt ggtcggcgat ctgtcgcttg gcggcggctt tttgcgctgt
     2581 caatcgagcg gcgtgggcgg tgcgctggtg atttcggccc ggcccacgat cgaaggcggc
     2641 ggcgatctgg tgctgcgtga cattcgtacc agcgtcgaac ccgtcggaca gatccagtgt
     2701 ggcggtctgc cgttgccggt ggcggatgtg ttttctgcga tgctgcgtcc gctacagtcg
     2761 ctggcccagc agcttggtca gctcgtcaag attccgctcg gtccggtgct ggcgcaggga
     2821 atcgccgagc tgtcgacgcc gcggcctttg gcgatggctg gccgtaagac ctgtttggat
     2881 ctgcatccgt cggcgctggt gctggcccca ccgagcgcgg cacctgatgg atcgcatgcg
     2941 atttcggtgc gaatcggtct ggatgtcgag ccgcggctct ctttgggcga ctgtccgagc
     3001 gggggagggc ggacgccggc ttctctgacc gtgcggcaag aaaacctcgg ggaagagttt
     3061 gccatccagg tcgccgtcgc tgtgccgatg tctgatctga aatcaatgct tgggccgcag
     3121 cttgtcggca agcggctggg cagtgcggcg cagtcgctgc tcgttcaggg cgtcgaggtc
     3181 ggtgatgcaa atggccgggt gctgcttcgg cttgatgtcg ccggcatcta caccggcgcg
     3241 ctgtttttgt ggggcacgcc aaagcttgtc agcgaaggtg gacggcagat tctccaggtg
     3301 cccgacttgc aagtcgcagc cgagtccagc tcgaagctgc aagagctcaa agtggctgct
     3361 tatgagcttg tcgaaggcaa tctggccgac aaagtgcggc cccatctgcg acttgatgtg
     3421 acggacaagc tggcagagtc gcagcgtgcg ctgtcaggaa ccatcaagct gcaaggcggc
     3481 gcttggcaga atgtccagcc ggcagcgcaa gcgctgggca tttcgcaggt cgcgctgcag
     3541 accgatttgt cgcaggtgtt gccgctcgcg gtcgaagcca aacccggtgt ggtggtcgcc
     3601 tacgtgctgt tgcgtgggcg ctcgctgctt tcgattcagt aggtcaaaag agcaggccaa
     3661 agccatggca aaattgtcag tctgcggcgc aaagtggtag gagtcccatc gtgaaaagca
     3721 aaaccaagaa gcttaccgga accgttcacc acgtcgacct ggaaggcggc tactttacgc
     3781 tgcggcttgc ggggggcgaa atctacaagc tcgatggtgg cggagccgat ttgcgcaaag
     3841 ccggggtcaa agccgaagtc gaaggtgaaa tcgaagacaa gggctttggc atcagctttg
     3901 gaacgccgac tgtgaaagtg aagagctaca aggttctgta agcaggcacc aatatgaagc
     3961 agatcaaatg cttcatattg aagatgcgtt accgacggac cagcctgccg ctacagcgtt
     4021 ggtacttggt aggaaagcag cttttccagc tctgattcgg cttgggttcc gcccagcttg
     4081 gaatgcaaaa gccggttgcg gaaccacgac aaaacgccgt cttccagcgt cagaactttt
     4141 cccgcgtagt acgaccgcgt ggtcataagt gtcgtccgct cgcgccgggc cgtttcgtag
     4201 tgccgcagcg ctttgacctc gtttggttcg tcgcgcagac agcgtgccag caccaccgca
     4261 tcttcaatcg ccatgcaggc gccttgaccc aggttcggtg tcatcggatg gcaagcgtcg
     4321 ccgagcagtg tcactcgacc atccccaaag tgcagcagcg gcgcgcgatc gtagacatcg
     4381 tggcgcaaga tgtctgcttc gggggtctgg tgcagggcat ggacaaacgg ttcgggaaag
     4441 ccggcaaacg tggtcaggca gtcggctttg cgtccgcgcg gagagtcggt cttttgggcg
     4501 ggctggttgt gggtggcaaa ccagtagatt cggcccatgc cgcagggaaa caggccaaac
     4561 tccgtgccgt ggccgatggc ggtaagcgcc agccccgggg gcagcgtgcg gtggaccatc
     4621 gaaacgacgc tgcggtaaca ggtgtagccg gaataccgag gttcgtcagc gccgacaagc
     4681 tgcttgcgaa tcgccgattg aaatccgtcc gctgcgacca ccaaatcgcc gcggtgaagc
     4741 tcgccgtttt caaaaatggc ggtcacggtc gcttcgtcct gcgcgacgcc ggtgcactgc
     4801 atgccggtct gaatgttggc ggcaccgacg gcgtttacca aaatttggtg aaagtcggcg
     4861 cggtggatgc agaccgaagg gacgccaagt cggcggctga tttcgccgat gtgaacgtcg
     4921 ctcaaaagct cgccttccgc ggtctgtgtg cgggtgatgt cgacgaccga gcctatccgc
     4981 agcacttcgt ccgaaagacc aagtacgtgc agcgcccaca tcgcgttgct ccacagcgac
     5041 atgccagcac ccaaggcacg gacttcctgt gtccgctcga agatgcgcac ggtgtagcca
     5101 actttgcgca gcgcaatggc ggtacacagc ccgccgatgc ctgccccaat gatgagcgct
     5161 cgttttccca tcccgatgtt gcgttcccta gtacgacgcc gacaggctaa gcccaagaat
     5221 gtttgcgccc tgtttgtcgc gacccaccat cggggcaagc gataccgcag gggccgcagg
     5281 tggtggggct tccggtgccg ccgtccgcgc tttgtgccac atgtgggcat tccaaatcga
     5341 cagacctagg ttcgtcagac cgacgacacc gtgcataagc cccatgccga tgccgtattc
     5401 gactttcggg tcggtgccgt agatcatcac gatggggctc gtcgcggtgt tgataaagcc
     5461 gcagatgaag ccgctgtaca tccaagcgcg actcggcgtc tttcggaccc ccgtgacggc
     5521 atttccgacg atcgatgtca ggctgcacgt cgccatcgcg ccttccaaaa tgaaggccat
     5581 ctgctgtccg ggcaggacga tgatgtcacc accagcgcgc gccggacggc tgtcacagac
     5641 cagagcggcg agcaccaggg tgccgcagag tttcaggctg gctcgattca tttctgtccc
     5701 tccggggccg gggtcgtcgt ggcgtctgcg ggcgcggggc tggctgtggg gctctgctgc
     5761 gccgccgggg ccgctggggt ttctgacagc gtggtggtcg ccgacgtggc tgcatccgtc
     5821 ggagcggctt gttgttcttc ttcggcgcga cgggtcggtg tgctcgaaag ttcgtaagtg
     5881 acgtcaagcc cagccgcgag cgtgatcccc gaacggaagg cgtggtgcgt gaaagtaccg
     5941 acgcgcataa agaaagcgaa gctcttggcc agatcgcgca ggttgctgac cgaccacgcg
     6001 aggatgccgg cggcgatgtt gcccttatat ccgctgccgg ggatgtagag caggttgtcc
     6061 gtggcgccga tgcgaatcgc gtggtttttg tgcggcttga tggaaacgat aagtgcggcg
     6121 ttgttgacca gggcccagtc gccgcgaccg tccatgatgc cgtcgagctt ggtgatgtac
     6181 caaaacggcg aggcaaagtc tccgttggcg gcgcgccaaa actcgccggt cagtccgttg
     6241 acaatcagga tgtccaccca cttgccaagc ggcagcgcgc cctgaagcgt gacgccaagt
     6301 gccgtgcgca gaccgaacat gtctttggca acgccaagcg cgggcttgac cgtctccgcc
     6361 ttgaagtcgt cgtagccctg cacttcgatg tagccggcgc cttgcagcgg gatcggccag
     6421 atgtaaaagc cagtcagctc ggcgcgcaga atcaaaaacg acagcggggc aaaccgaacc
     6481 gtcggtccga tgtgaaagtg cgtcggcgaa atgtagttcg cgagcccaaa ttccagcttg
     6541 gtgtagtccc acaggatgcc gggcttttcg acaaacggta agcagtacgt caggatgagc
     6601 tggttttcga tgccgagcgg gtttatcgat ccgcccatga agtgttcgag catcagcttg
     6661 cgcttctgct cgtcgttgca gtgcttgccg cgggtgccaa aaggtccggc agtggcgcgc
     6721 agcgtcttgg tctgggcgac ggcttggctt gcgatcgcga acagaccgcc taccacaagc
     6781 agcgtcgcga aaacaccgcg tgtgagcatc gctcggagcc agcttcgccg gcttgcccga
     6841 ggcctggtac agcttggttt cttacacatt tgggacaggg tactgcaagc gattgggctt
     6901 tgtcatgaac cggtaagcgg accgccttcg cgcggcattt tccggcggaa aaccgagagt
     6961 caaaggcgac caaacagcgc cctatcgttt tttaccaact cggagtagac tcccagcccg
     7021 gtttaacaga cggactacgc aaacacgacg gctgcccgtc ccggttggga tgccttgggc
     7081 cgccggacca aaggagactt tcatccatgc accacaccac ttccggtcgt aagaagatcg
     7141 ccgtcatcgg tggcggcaac atcggtggcg agctggccgc gctcattgct cgcaaggagc
     7201 ttgctgacgt agtcctgctc gacattcccg aaaaagatgc gttttgcaaa ggcaaggcgc
     7261 tcgacctgat gcaaaacggc acgctgctcg gctgggactg caacatcacc ggcaccggca
     7321 actgggcgga cgccgcgggc gcaaacgtcg tcatcatcac cgcgggtgtg ccgcgcaagc
     7381 cgggcatgag ccgcgacgat cttctgtcgg tcaacctgaa gatcatgcgc agcgtggccg
     7441 gaaacatcaa aacccactgc cccgactcgt tcgtgattgt gatctcgaac ccgctcgacg
     7501 caatggtcta tgagtgcgcc aaggtcactg gctttccgaa gaaccgcatc tgcggcatgg
     7561 ccggtgtgct cgactcggcg cgcttccagc tctttttggc ccaggaaatg ggcgtcagcg
     7621 tcaaggatgt caaggcgatg gtgctcggcg gacacggcga caccatggtg ccggtcacgt
     7681 cctactgcac cgtcaacggc attccggtca agcagttcat ctcgccggag cgtctggccg
     7741 caatctgcgc ccgcaccgct ggtggtggtg gcgagatcgt caagctgatg ggcacttcgg
     7801 cgtactatgc cccggcgacg gcggctgtgg tgatggccga gtcgtttttg catgaccaaa
     7861 agcgcctggt tcccgctgcc gtgtactgca acggcgagta cggcatcagc gacctgtaca
     7921 tcggtctgcc ctgtgtgatt ggcaagggcg gcgtcgaaaa ggtgctggaa atcacgctca
     7981 ccgacgatga aaaggcgcag cttggcaaga gcgtcgccgc cgtccgtgag ctggttgatg
     8041 ccagcgtccg gctgtaatct gccatcccgc tttcaccaaa accaacagcg agtcactgcc
     8101 taagcccgtc tgctcaggtg gacgagggcc cagatggggc agtgactccg tcaaggggta
     8161 gcccatgaac attcatgagt atcaggccaa agagcttttg cggaagaagg gcctgccagt
     8221 gccggtgggc aagtcggctc tgtccgtgga cgatgccgac agcgcagcag cgtgggtgcg
     8281 cagcgaaacc ggcaatccaa tcgtcgtcgt caaggcgcag attcacgctg gtggccgtgg
     8341 caaaggcggc ggcgtcaaag tcaccaagac cgccgaagac acccaagcgc agattcgcaa
     8401 gattttcggc atgcagcttg tcacgcacca gaccggcccc gaaggcaaga aagtggggcg
     8461 gctgcttctg gaacaaggcg tcgacatcgc caaggagttc tatgcagccg tcgtgctcga
     8521 tcgccagaag ggccgcttgg tcctgatggt gtcgaccgaa ggcggcatgg acatcgaaga
     8581 agtcgccgcg catcagccgg aaaaaatctt caaagcggtg atcgacccga tgaccggctt
     8641 tacgccatac caagcgcgcg aagtcgcgtt tgcgctgggt ctgaagtcgg cgggcatgga
     8701 agtcgtgaac agcgcctgca agttctttgc gacgatggtg cgggcgtatc aggagcttga
     8761 ctgctcgctt ctggaaatca atccgctcgt gctgaccaaa cagggtcaga tcattgcgct
     8821 ggatgcgaag atcaacttcg acgacaacgc gctgtatcgg catcccgata tcgaggaaat
     8881 gcgcgatctc gaagaagagg aaccggcaga gacggaagcc aagaagtttg acctgtcgta
     8941 tgtgaagctg acaggcaaca tcggctgcat ggtaaacggc gccggcctgg cgatgtcgac
     9001 gatggacatc attcgcttct acggcggcat gccggccaac ttcctggatg tcggtggcgg
     9061 cgcgacggcg gaaaaagtga ccgcggcgtt taagatcatc actgccgacc cggcggtgcg
     9121 cggcatcttc atcaacatct tcggcggcat catgaagtgc gacaccatcg ccaaaggcgt
     9181 gatcacggcg gtgaaggaag tcgggctgaa agttccgctg gtggttcggc tcgaaggcac
     9241 caacgtcgag ctgggcaagc agatcctggc cgagtcgggg cttaacgtgg tggcggcgtc
     9301 cgatatggcc gacggcgccc agaaaatcgt cgcgctcgcg ggcgagtcgg cggcataggg
     9361 aggcagtcat gtcgattctc gtaaacaaga acacccgcct ccttgtccag ggtctgacgg
     9421 gcaaaaacgg aacctttcac accgaaaaag cggtcgagta cggcacgacg gtggtcggtg
     9481 gtgtgacgcc gggcaagggc ggcagcacgc acctgaacct gccggtgttt gacacggtcg
     9541 ccgatgcggt caagcagacc ggcgccaatg cgtcggtgat ctatgttccg gcggcgattg
     9601 cggcggacag catcctcgaa gcgattgcgg cggggctgga actggtcgtc tgcatcaccg
     9661 agggcattcc ggtcatggac atgctcaaag tgcggcgtgt gctcgatgcg cagacgggac
     9721 agaagcgcac gcggcttatc gggccaaact gccccggcat catcacgccg ggcgaatgca
     9781 agatcggcat catgccgggc tacatccaca agccgggtca catcggtgtg g
//
LOCUS       DAORGN010000184         9821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_2974_length_9821_cov_5.342105, whole genome shotgun sequence.
ACCESSION   DAORGN010000184 DAORGN010000000
VERSION     DAORGN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_9821_cov_5.342105"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKL17_17000"
     CDS             <1..776
                     /locus_tag="PKL17_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46483.1"
                     /translation="IPSDTPQTQGIESIISTENSTATGQFSILCRINSAIERLTINSQ
                     LTPQFAKTENHKSWSLVSGNNGTLHAVIEYLPSISKDTFYQSLSVSTSPVFSNSNLAP
                     VMPTNTESISKIIVGKFPSKYDGAIAWSANGTNVYYSILNDPINNIKYNTLSKTSQYP
                     QISGCNDSALELCSVALADIIDGDAGELIVARHNGIMIYKDGTSNNTIFIPTETEPQA
                     VSVSINNDKNMRKLIWASSKFENNKNTVTIHTIPVSSIM"
     gene            800..2437
                     /locus_tag="PKL17_17005"
     CDS             800..2437
                     /locus_tag="PKL17_17005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46484.1"
                     /translation="MMSTLKPGDVIDDTYQVERLLGSGGMAHVYVVKHLRMPRKFALK
                     VLQIEVAVRRDFLERFEREAEILATLRHPHIVDVIDRNKLPDGNPYLVMELLEGEDLA
                     TFIARSGALSVPVALRIANQIGEALEAAHHVGITHRDLKPSNIFLCKQGHLLNFVKVL
                     DFGIAKLAVSNRAPMTAPAQIMGTPGYMAPEQARGEGHRIGPATDQFALAATLYEMLV
                     GQGPFYRAGDTLFATIERVVHAKPEPLPDPQINASVQRALSKEPENRFPTLQSFLAAV
                     GASTHTVYDMPPLTLRPSALTSLAGESARITVGFTFSRRRLGIFAGALCLFGAALYAG
                     SGLLHSAHSQARDAHSAVGQHPGAAGAIPEPIPAPIPAPTSVVKTENNAQMAATPPEV
                     GTHVDAGAQQAERVAEKSDDPDDQEPSRRNTTVAETDAGPETEDPEVPEPPSGKTAGA
                     NRTPKARSPVRVIPSIKPARLGRIYELVEFCNKGILARLPLQDGTLIRLQRAGTLTVV
                     AAPASVIRSGFNECLRQQLAEVAPVLLPEKLTLTIRR"
     gene            2449..3123
                     /locus_tag="PKL17_17010"
     CDS             2449..3123
                     /locus_tag="PKL17_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46485.1"
                     /translation="MQIKRLLLPLLCLFLCNCYAGELGHIRNVMSAMQSEIATMRHAQ
                     EEFKAQFSDGLTLALCSPELKQLLEDVKQECAAQSKRDKIGSCSTNQIKPAVIGADPE
                     HRGRFLKMMSHLRHEVVYIGNKSKSVLPYRSLRIEKLARPALLDHTVFLVVSSPELNE
                     DEAIRRAIFVEQLLLDDGVPADRMKRWIYSFPAKSQDIVNSVDHPSIAETKELSRGVW
                     IFRSDC"
     gene            3092..3718
                     /locus_tag="PKL17_17015"
     CDS             3092..3718
                     /locus_tag="PKL17_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46486.1"
                     /translation="MAYGFSARTVSRFVVLTSLCWSAVGLAASPDDVPQADQESSLLH
                     RKIDLSNCQRWDRTDRRWVPYSLRPAKVYVVNLWSPICKPCRDEFPQFRSMANGWRSH
                     PEVQFVFLSDPPDLATKADVANYWQEHQAELPDGDPVRTDTSVVRDTLGTARNPITLI
                     VDENLIVRQAFVGAIGDRPLVRSIERYLQTAMPTFALSGKSKRTTKQR"
     gene            3849..4397
                     /locus_tag="PKL17_17020"
     CDS             3849..4397
                     /locus_tag="PKL17_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK46487.1"
                     /translation="MTAVFDLQPVLVGTLLTCRPLRRDDCDSLYQIAADPLIWEQHPQ
                     PTRYQRPVFEQYFEGAMRSGGALAVLHRTTGEPIGCSRYYDLAADRSQIIIGYTFLAR
                     KYWGGEYNRELKQLMLVHAFRFVDTVVFEIGVSNHRSRRAIEKIGAILKREQSHDGST
                     HAIYQIHRHDFLPLHKPNGEPP"
     gene            complement(4614..6788)
                     /locus_tag="PKL17_17025"
     CDS             complement(4614..6788)
                     /locus_tag="PKL17_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNK46488.1"
                     /translation="MRCAWLAWLVVAMLFCHRDGAWAQSPSGTGPRRFTLLVASTDGG
                     PGRARLRYPSKDTESLQRVLLEIGGVDGHDIETLVDPDGATVEKKLLALRQKAAQTPG
                     PKQFFFFYSGHSDERGLLLFGSHLEYAKLRSLITDIPVDVHIGILDSCAAGAFAQRKG
                     GAPVDPFLVQVAPNLSGHAFLTSTSEREAAQESGRLRGSFFTHALVTGLRGAADSDGD
                     QRVTLQEAYRFAFDETLSRTEATLGGPQHATYDIQLVGRGDLVLTDLRAGSARLYIPE
                     GIQGRVIVRDSRGSLAAEVSKTSGQTSLALSLQPGPYRITLFESQRVRRGQVSLPPQG
                     LVLAQSELRDVPIELATARGSSLEAVEASEATAPSDADPSYRVLPGNAGIVPQWSVNA
                     IEKQRPVVNYFSLNLGWGKAARLYGAELGIVGAYVTEEVAGLQGAALFAYSGGRFRGA
                     QASFGANIIRGDGFGAQLGAVNVATADITGLQASTVNYSGGDLRGLQIAAVNIAKGGV
                     YGLQASSVGYAARMYGLQLGGINIAGQVAGMQVAGINIASGRVRGVQLGVINIADDAD
                     VAIGLFSISKKQGAYVDLWMSDSAAINVSIRLPARYSYSIVSAGVHPAGAGAGWQYGL
                     GFGFHAPLSERLYGEIDDTVFGVQSGFATLEMPSLLNVTRIMLGVRIARRFAVFFGMT
                     FNMQWGFKQDEDPVRPGFDLGSASMPIPHWRLWPGFAFGMTI"
     gene            complement(6785..7570)
                     /locus_tag="PKL17_17030"
     CDS             complement(6785..7570)
                     /locus_tag="PKL17_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ActD-like protein"
                     /protein_id="HNK46489.1"
                     /translation="MTKPNPNPLQIEQALLGELPKGTVLPDGSEAQIEALRQENQQIL
                     DRYPAARIAKEVQRRFDSEKAPQRRWSMVLLPAAAVPLVLAAVLWGPIGKVPPSGQGG
                     DESTAVGMDTDVQLKGLSPELRIYRKRDGVAPERLVDGSVVHSGEVIQVAYLAAGRSL
                     GVIVSIDGSGNVELHHPASETGAASLMRGTEQPLPKGYKLDAAPRFERFFFVTSKTPG
                     SLDVLQVVAAARSLAAKPDADRSALAVSSALEVSSLTLRKEAP"
     gene            complement(7567..8049)
                     /locus_tag="PKL17_17035"
     CDS             complement(7567..8049)
                     /locus_tag="PKL17_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK46490.1"
                     /translation="MAIDVEELYTRYGPMVLRRCRSMLRSEAEAVDAMQDVFVELLRR
                     RERLLADSGSSLLYRVATNVCLNRLRSRSRRKEEPGDALLERIAIASSEDRSEARALI
                     DRLLSREPVSSGTIAVLHLHDGMTLEEVAKEVGLSVSGVRKRLRKLHERLRELEGQSP
                     "
     gene            8280..8615
                     /locus_tag="PKL17_17040"
     CDS             8280..8615
                     /locus_tag="PKL17_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46491.1"
                     /translation="MKNASPNPQQSPRTVQPGAASGNRITLDDRSEVVPALFGGRYQL
                     QGLIGAGGTGTVYRALDIELGEVVALKVLRREVVSLPRVLDRFRSEVRLSRRITHRNV
                     ARMYDICAA"
     gene            complement(8725..>9821)
                     /locus_tag="PKL17_17045"
     CDS             complement(8725..>9821)
                     /locus_tag="PKL17_17045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46492.1"
                     /translation="QVADVLAAAHIKGIIHRDLKPENVMLLPDAATATGERAKVLDFG
                     IAKLIEANNQASVQTRATELLGTPNYMSPEQCRGAKGVDEKTDVYALGVMMFEMLVGR
                     PPFVGTALGELMAKHMYEPAPKVAACNPQVPEVLANLVDVLLDKDKEKRPSMAALEVQ
                     LEQMTAELPPPAKHRHRNSGAMAVVQSGRHAHPDAKTVAGTTPTTPGTTGVVDKQRRS
                     VVWIGLGIALSLTAVGGGVFGLLATRKKPDPKPEAPTVAKRIRWHLRSVPAAAQVVRV
                     SDGVVLGKTPLDQEAEQSSEPIRLRVEAAGFLPQDVQISLSASGEVQVVLRPEPPKPP
                     VEAPPAVEQKPKQPTKKTKQKRVEVPHIEN"
BASE COUNT         2150 a         3018 c         2725 g         1928 t
ORIGIN      
        1 acattccaag tgacacacca caaacacaag gaatcgaatc aataatctca accgaaaaca
       61 gtacagcaac agggcagttt tccattctat gcagaattaa ttcagcaatc gaacggctaa
      121 cgatcaattc acaactaaca ccacaatttg caaaaacaga aaatcacaaa tcttggtcat
      181 tagtctcagg aaacaatgga acactgcacg cagtcatcga atacttacca tcaataagca
      241 aagacacttt ctaccaatct ctcagcgtta gtacttcgcc ggtattttcc aattcaaatc
      301 tagcaccagt catgccaaca aatactgaat caataagtaa aatcattgtt ggtaaattcc
      361 catcaaaata cgatggggca attgcgtgga gcgcaaatgg aacgaatgtc tattattcga
      421 tattaaacga cccgataaac aatataaaat acaacacact aagtaaaaca agtcagtatc
      481 cacaaatttc tggatgcaac gactcggcac tagaattatg ctcggtcgca ttggctgata
      541 ttatcgatgg agatgccggc gaactgatcg ttgcgcgtca caacgggatc atgatctaca
      601 aagacggaac cagtaataac acaattttca taccaacaga aacagaacca caggcagttt
      661 ctgtgtcgat aaacaacgat aaaaacatgc gaaaactgat ctgggcgtca tcaaagtttg
      721 agaataataa aaacacagtc acaattcaca caattccagt ttcgtcaatt atgtaaccga
      781 cgaagaccaa gcgggggcga tgatgtcgac actgaagcct ggcgatgtga ttgatgatac
      841 gtatcaggtg gaacggttgc ttggctcagg cgggatggcg catgtgtatg tggtgaagca
      901 tctgcgaatg cctcgtaaat tcgcgctcaa agtgctccag attgaagtgg cggtgcgccg
      961 cgattttctg gaacgatttg aacgcgaagc cgaaattttg gcgacactgc gtcatccgca
     1021 catcgtggat gtcattgatc gcaacaagct ccccgatggc aatccgtatc tggtgatgga
     1081 gctactcgaa ggagaggatc tggcgacgtt tatcgctcga tcaggggcgc tttccgttcc
     1141 ggtggcactg cgcatcgcca accagatcgg agaagcgctg gaagccgccc accacgtcgg
     1201 aatcacccat cgtgacctga agccgagcaa catttttctg tgcaagcaag gccacctcct
     1261 caacttcgtc aaagtcctcg actttggcat tgccaagctg gcggtgagca atcgggcgcc
     1321 gatgacggct ccggcgcaga tcatgggaac gccaggatat atggcgccag agcaagcccg
     1381 cggagaagga caccgcattg gcccggcgac ggatcagttt gcgctggcag caacgctgta
     1441 tgaaatgctg gtgggacagg gaccgtttta ccgcgctggc gatacgctgt ttgcgacgat
     1501 cgaacgggtg gttcacgcca agcccgagcc gcttcctgat ccacagatca atgcatcggt
     1561 tcagcgcgcg ctcagcaaag aaccagagaa tcgtttccca acgcttcagt cgttcctggc
     1621 agcggtgggg gcgagcaccc ataccgtgta cgatatgcca ccgcttacgc tgcggcccag
     1681 tgcactgacc agcttggccg gggaaagtgc gcggatcacc gtgggcttta ccttcagccg
     1741 acggcggctt gggatctttg ctggggcact ctgtctcttt ggagccgctc tctacgcagg
     1801 ctctggactt ctgcattcgg cgcacagtca agccagggat gcgcacagcg cagtgggaca
     1861 gcatccgggc gcagcagggg cgattccgga accgattccg gcaccgattc cggcgcctac
     1921 ttcggttgtg aagactgaaa acaatgcgca gatggcggcc actccgcccg aagtcggcac
     1981 gcacgtcgat gccggagccc agcaagcaga acgtgtggca gaaaaatcag acgatccaga
     2041 tgatcaggaa ccaagccgtc gaaacacaac tgttgccgaa acggacgctg gaccagagac
     2101 ggaagatccc gaggttccag agccccccag tgggaaaacg gcaggggcaa accgaacgcc
     2161 caaagccagg agccctgtcc gggtgattcc gtcgataaaa ccagcccggc ttggccgaat
     2221 ctatgaactg gtcgagttct gtaacaaagg aattctggcc aggctaccgc tccaggatgg
     2281 gacgctcatt cggctgcagc gagccggaac gctgacggtc gtggccgctc cggcttcggt
     2341 catccgatct gggttcaatg aatgtttgcg acaacagctc gccgaggtag cgccggtgct
     2401 gctgccagaa aaactgacgc tgacgattcg acggtgaaag acaaaaagat gcagataaaa
     2461 agactcttat tgccactcct gtgtctgttt ctgtgtaact gctatgcggg ggaactgggg
     2521 cacatccgca atgtgatgag tgcgatgcag tccgaaatcg cgacgatgcg tcacgcccaa
     2581 gaggaattca aggcgcagtt ttccgacgga ctgacgctgg cgctgtgcag tccagagcta
     2641 aaacaactgc tggaagacgt caaacaggaa tgtgcggcgc agtcgaaacg ggacaagatt
     2701 gggagctgta gtaccaatca gatcaagccc gcggtgatcg gcgccgatcc cgaacaccgc
     2761 ggtcgattcc tgaagatgat gtcccatctt cgtcatgagg tcgtatacat cggcaacaag
     2821 tcgaagtccg tgctgccata ccgcagtctt cggatcgaaa aactggccag accagcactg
     2881 ctcgatcaca cggtcttcct ggtggtttca tcgcctgaac taaatgaaga cgaagcaatc
     2941 cgccgcgcca tcttcgtcga gcaactgctt ctagatgacg gagtacccgc cgatcgaatg
     3001 aagcgctgga tctacagctt cccagccaaa agccaggaca ttgtgaattc cgttgatcac
     3061 cccagcatcg ctgaaacaaa ggagctgagc cgtggcgtat ggattttccg ctcggactgt
     3121 tagtcggttt gtcgttctga cttcgctctg ctggtctgcg gttggtcttg cggccagccc
     3181 ggacgatgtt ccgcaggcgg atcaggaatc gtctctgctt caccgcaaaa ttgatctatc
     3241 gaattgtcag cgctgggatc gcacggatcg tcgctgggtt ccctattccc tgcgtccagc
     3301 caaggtgtat gtcgtcaatc tgtggtcgcc tatctgcaag ccctgccggg atgagtttcc
     3361 gcagttccgt tcgatggcaa atggctggcg ttcccatccc gaagtgcagt ttgtgtttct
     3421 gtccgatcca ccggatctgg cgaccaaggc cgacgtggca aactactggc aggaacatca
     3481 ggcagagctg ccagatggag atccggtccg caccgataca tcggttgtgc gcgacacgct
     3541 gggcaccgca aggaacccga tcacgcttat tgtcgacgaa aatctgatcg ttcgtcaggc
     3601 atttgtcggc gccattgggg atcgtccgct ggttcgatcg attgagcgct atctccagac
     3661 cgcgatgccc acattcgcgc tgtccggcaa gtcgaagcgg acaacaaaac aacgatagcc
     3721 ttgtccgtga atagagcgag aggcgtagca aacgcaggtt gaagcgaagc ggagactgcc
     3781 cgtgtgacga gggtggcaaa agcatctggt aacgaacggt cgtgatatga gacatcgcgt
     3841 gaaccacgat gactgccgtc tttgatcttc agcccgttct cgttggtacg ctcctgactt
     3901 gtcgccccct ccgtcgcgat gactgcgatt cgctgtacca gatcgcagcc gatcccctga
     3961 tctgggagca gcatccacag cccacgcgct atcagcgacc cgtcttcgaa cagtattttg
     4021 aaggagcgat gcggtccggt ggcgcactgg cggtgctgca cagaaccact ggcgagccaa
     4081 tcggctgctc tcgctactac gaccttgctg ccgatcgaag ccagatcatc atcggctata
     4141 ccttcctcgc caggaagtac tggggcggtg aatacaatcg cgaactgaag cagctgatgc
     4201 ttgtgcacgc cttccgcttc gtggacacgg tggtgttcga gatcggtgtg tcaaatcaca
     4261 gatctcgacg tgcgatcgaa aagatcggag caatcctcaa aagggaacaa tcccacgacg
     4321 gcagcacgca tgcgatctat caaatccaca ggcacgactt ccttccgctg cacaaaccaa
     4381 acggggagcc gccatagcca gtccgtccat gctttggctg ccgcaccaaa cgccttcccg
     4441 ccgacttccc acatggccgt gcagagcccg tgaccccgac tttccccccg cgatttcgcc
     4501 ctggattttt ccgacggaag ctcagccggg acacacaccc gatgaactca ttccggcggt
     4561 ttctgcgggg gttgggaatc gggttgggat ttggggaacg gctgcgaaac ggattagatg
     4621 gtcatgccga atgcgaagcc gggccacaga cgccagtgcg gaattggcat cgacgcggaa
     4681 cccaggtcaa agccaggccg caccgggtct tcgtcttgtt tgaaacccca ctgcatgttg
     4741 aacgtcatgc caaaaaacac cgcaaatcgg cgcgcgatcc gcacaccgag catgatgcgc
     4801 gtgacgttca gcagcgacgg catttccagt gtggcaaaac cggactggac gccaaatacg
     4861 gtgtcgtcga tttcgccata cagccgctcg ctcagcggtg catggaaacc aaagcccagt
     4921 ccgtactgcc agccagcgcc cgcccctgcc ggatgaacgc ccgcggacac aatcgaatag
     4981 ctataacgcg ccggcagacg gatgctgacg tttatcgcgg cgctgtcgct catccacaaa
     5041 tcgacgtagg cgccctgttt tttcgaaatc gaaaacagac caatggcgac atcggcgtcg
     5101 tcggcaatgt tgatcacgcc aagctgcaca ccacggacgc gaccgctggc gatgttgatg
     5161 ccggcgacct gcatgccggc gacttggccc gcgatgttga tgcctccaag ctgaagtcca
     5221 tacatgcgcg cggcataacc caccgacgac gcttgcagtc cgtagacacc gcctttggcg
     5281 atgttgaccg ccgcgatctg gagaccgcgc aagtctccgc cggaatagtt caccgtcgac
     5341 gcttgcagac ccgtgatgtc cgcagtagca acattgaccg cgccaagctg ggcaccaaac
     5401 ccgtcgccac ggatgatgtt ggcgccaaag cttgcctgcg cgccacgaaa ccggccaccg
     5461 ctgtacgcaa acaaggccgc gccttgcaga cccgcgactt cctcggtgac atatgcgccg
     5521 acgatgccaa gctcggctcc gtacagtcgc gccgctttgc cccagccgag gtttagcgaa
     5581 aagtagttta cgaccgggcg ctgtttttca atcgcgttca ccgaccactg cggaacgatg
     5641 cccgcattgc ccggtaacac ccgatagctg gggtcggcgt cactcggcgc cgtggcttcg
     5701 cttgcttcga cggcttccag actggacccg cgcgcggtgg ccagttcaat cggaacatcg
     5761 cgcagctcgc tctgcgccag gaccagacct tgcggtggca gcgatacttg tccgcgacga
     5821 acgcgctgcg attcaaacag cgtaatccga tacggccctg gctgaagcga cagcgcgagg
     5881 ctggtctgtc cactggtttt gctgacttcg gcagccagcg aaccacgact gtcgcgcaca
     5941 atcacacggc cctggattcc ttcggggatg tacagacggg ccgaaccagc gcgcagatcc
     6001 gtcagcacca aatcgccgcg cccgacgagc tgaatgtcat acgtcgcgtg ctgcggtccg
     6061 cccagcgtcg cttcggtgcg cgacagcgtt tcatcgaatg caaaacgata ggcttcttgc
     6121 agcgtaaccc gctgatcgcc gtcgctgtct gctgccccgc gcagtcccgt gaccagcgcg
     6181 tgggtgaaaa acgaaccacg cagccgtccc gattcctgtg cggcttcacg ttccgatgtc
     6241 gaagtcagaa aagcatggcc gctcaggttc ggcgcaacct gcaccaaaaa tgggtcgacg
     6301 ggagcaccgc ctttgcgctg cgcaaatgcg cctgctgcac acgaatcgag aattccgatg
     6361 tgaacgtcga cgggaatgtc ggtaataagc gatcgcagct tggcgtattc cagatgactc
     6421 ccaaacagaa gcagaccgcg ctcgtccgag tgaccggaat agaaaaagaa aaactgcttg
     6481 ggccccggtg tctgcgccgc tttctgacgc agtgcgagca gctttttttc caccgtcgcg
     6541 ccgtcggggt ccaccaatgt ttcgatatca tgcccgtcga cgccaccgat ttccaaaagg
     6601 acgcgctgga gcgattcggt gtctttgctg ggataccgca gccgcgctcg cccaggtcct
     6661 ccgtcggtgc tcgcaacaag caacgtgaag cgccgcggtc cggtgcccga tggcgactgc
     6721 gcccaggctc cgtcgcgatg acagaacagc atcgcaacca caagccaagc cagccacgcg
     6781 cacctcatgg ggcctctttg cgcagggtca gcgacgacac ttccagcgcg gatgacaccg
     6841 ccagcgccga tctatcggca tccggttttg ccgccagcga ccgcgccgca gccaccactt
     6901 gtaaaacgtc gagcgaaccc ggcgtcttgc tggtgacaaa aaagaagcgc tcaaaccgtg
     6961 gcgccgcgtc gagcttgtag cctttgggca gtggttgttc ggtcccgcgc ataagcgatg
     7021 cagcgccagt ttccgacgca ggatggtgca gttcgacgtt gccgctgcca tcgatcgaaa
     7081 caatcacccc cagcgaccgt cccgccgcca gatacgcaac ctggatgact tccccggaat
     7141 gcacaacact gccgtcgaca agtcgttccg gggccacacc gtcgcgcttg cgataaatgc
     7201 gcagctctgg cgacagccct tttagctgca cgtcggtgtc catcccgacc gcggtcgatt
     7261 catccccgcc ctgccccgat ggcggcacct tgccgatcgg cccccacagc accgccgcca
     7321 gcaccagcgg aaccgccgcc gcgggcaaaa gaaccatcga ccagcgacgc tgcggagcct
     7381 tctccgaatc gaagcgacgc tgcacttctt tggcgatgcg cgccgcggga tacctgtcga
     7441 gtatctgctg gttttcctgc cgcagcgcct cgatctgcgc ctcgcttccg tcgggcaaaa
     7501 cggtcccttt gggcagctca ccgagcagcg cttgttcgat ttgcagcgga ttgggattcg
     7561 gcttggtcat gggctctgtc cttccagttc gcgcagccgt tcgtgaagct tgcggagccg
     7621 cttgcgcact cccgacaccg acagaccgac ttctttggcc acttcctcta gcgtcattcc
     7681 atcgtgcagg tgcagcaccg cgatggtccc acttgacacc ggctcacgcg aaagcagccg
     7741 gtcgataagc gcccgcgctt cacttcggtc ttcactgctg gcgatggcaa tgcgctcgag
     7801 aagcgcgtcc cctggctctt ctttgcgacg cgaccgactg cgcagccggt tcagacacac
     7861 gttggttgcc acgcgataaa gcagactcga tccggagtcg gccaaaagcc gctcacgccg
     7921 tcgcagaagc tcgacaaaga cgtcctgcat cgcatcgacc gcctcggcct cgctccttaa
     7981 catgctgcgg caccgtctca gaaccatcgg tccgtacctt gtgtaaagct cttcgacatc
     8041 gatcgccaac gcgccactcc agggcaccga ttaaacaccg gagccaagca aagttgttac
     8101 ttcgcaccgc atcgactttg cgcgaaaagc agccgcttgc caggcagccc ggcgcgacct
     8161 acccataaag aaactgtcca cggtttttgg acgcagccgc attgcgagac tgaaaaccgc
     8221 ggacgctcca tggccaggcc caagcccagc gcagagcctg ttaagatcgg cagccggtga
     8281 tgaagaacgc gagcccaaat ccccagcaga gtccccgcac cgtccagccc ggcgcagcgt
     8341 cgggaaatcg aatcacgctc gacgatcgat ccgaggtcgt gccagcgctg ttcggtggtc
     8401 gctatcaact ccaaggcctg attggcgcag gtggcaccgg caccgtctat cgcgcgctcg
     8461 acattgagct aggcgaagtc gtggcgctga aagtgctgcg acgagaagtc gtcagcttgc
     8521 cccgcgtcct cgatcgtttc cgcagcgaag tccgcctgtc ccgtcgaatc acccatcgca
     8581 acgtcgcccg gatgtacgac atctgtgcgg cctgaccgaa caatcgtcgg aaaaatggcc
     8641 tgattgttca gaccgtgcac cgcctgtgca ccgcgtatag aggtcggatg gatgcccacc
     8701 cggccaagcc ccttgcgggt acgcttagtt ttcgatgtga gggacttcga cgcgcttttg
     8761 tttcgttttt ttggtcggtt gtttgggctt ttgctcgacg gcaggtggcg cttctaccgg
     8821 cggtttcggc ggctccggtc gcagcaccac ttgcacttcg ccgctcgccg acagactgat
     8881 ctgcacgtcc tggggcaaaa atcctgccgc ttcgacccgc aaccggatcg gctcactcga
     8941 ctgttcagcc tcttgatcga gaggcgtttt gccgagcacc acaccatccg agacgcgaac
     9001 cacttgagcc gcagccggaa cggaccgcag atgccagcga atgcgcttgg ccaccgtcgg
     9061 tgcctcgggc tttggatccg gctttttgcg cgtcgccaaa agaccgaaca cgccaccccc
     9121 gacagcggtc agtgacagcg caatgcccaa cccaatccac acgacgctgc gacgctgttt
     9181 gtcgaccacc ccagtcgtgc ctggtgtcgt cggcgtcgtg ccggcaaccg tcttggcatc
     9241 gggatgagcg tgccgtccac tttgcaccac cgccatcgcc cccgagttgc ggtgccggtg
     9301 tttcgcgggc ggtggcagct ctgcagtcat ctgctcaagc tgaacctcga gcgccgccat
     9361 gctgggccgc ttttccttgt ctttgtcgag cagcacatcg accaagttgg ccaacacttc
     9421 cggcacttgc ggattgcagg cggccacttt cggcgcgggt tcgtacatgt gcttcgccat
     9481 cagctcgccg agcgccgtcc cgacgaacgg aggcctcccg accagcattt cgaacatcat
     9541 cacaccgagc gcatacacat cagttttttc gtcgacgccc ttggcccccc ggcactgctc
     9601 cggagacatg tagtttggcg tgcccaaaag ctccgtcgcg cgggtctgca cactcgcctg
     9661 gttgttggcc tcaatgagct tggcgatgcc aaagtccagc actttggccc gctcgcccgt
     9721 cgccgtcgcc gcatccggaa gcagcatcac attttccggt ttgaggtctc tgtgaatgat
     9781 tcctttgatg tgggcggcgg ccaggacgtc ggcgacttgc a
//
LOCUS       DAORGN010000185         9617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3080_length_9617_cov_5.878582, whole genome shotgun sequence.
ACCESSION   DAORGN010000185 DAORGN010000000
VERSION     DAORGN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_9617_cov_5.878582"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKL17_17050"
     CDS             <1..229
                     /locus_tag="PKL17_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK46493.1"
                     /translation="IVSKLAPPRWVGVMIGVWLLSTASGNLLVGLLGRYWEQWPHSQY
                     FAVIAAGLLVSALLLLSQLRWLRRVIPSQPS"
     gene            complement(207..3896)
                     /gene="mfd"
                     /locus_tag="PKL17_17055"
     CDS             complement(207..3896)
                     /gene="mfd"
                     /locus_tag="PKL17_17055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNK46494.1"
                     /translation="MDRHPDHVEDHAEPVSLDELPVSPLRAALPRLLSRISAGETRLR
                     TFGTVGPLQALLIAKLVDKLPQKSLPLVALVADENAARTLRKDLQFFLHRGQSTDDPA
                     ASDAVIGLPELDVTPWADASPERAAVLSRMSTLFRLSQASVLPPQVVVASVEALSRKV
                     VPKAAFADFVDIIAAEEELDRDQTIKRLAQGGYTRAPVCEDPGTYAVRGGVLDVFVPL
                     YRFPVRIEFFGDLVESLRFYDPTSQRTLRKTDEIYIHPVRETILTRGHKLRERLLEVG
                     DLAVHPSSRTRNILEQIESGTDFFGVESLIPAFHEKLSSIGEYLPEHSTFVVVEPHRL
                     FDSLSELHRDGESAYQKRIADHKLAFAPSEFYLDPTELRQLLLGSRAKPSLCVEIDPL
                     VITGEDDAVPAVRLCGEDHRLLAAELQRGQKEKHEHILKPLATVLRKNQDDGVRSVIV
                     AGSLQGAERLESLLRGYGLKPSLHRPGLSSLAVEQKSGEAVHHFDLLDSDPQLLGRIE
                     IRVGTLLRGVDLPLDRVSLYSESEIFGEKAVRKTAKASKKPSLGDLKNLEVGGYVVHP
                     LHGVGRYRGLTKLPVRTGGVAIDFMHIEYDGGQLYLPVWRLSEVQRYVGAEGVTPKLD
                     RMGGETWQKTRSKVIREVQQIAEELLKIYAQRQALPGHSFVLDSDADQLFAEFEATFP
                     FEETPDQARAISDVLGDMDLERPMDRLVCGDVGYGKTEVAMRAAVKAVLAGKQVAVLA
                     PTTVLTEQHATTFSNRMKTLPITVSSLSRFRSRAEQLKELKDLADGKLDIIVGTHRLL
                     STDVRFKDLGLVIVDEEQRFGVKHKERLRQFRSQVDTLTLTATPIPRTLHMALSGIRE
                     ISIISTAPADRLAIRTIVARESDDLIRDGIERELRRGGQVFFVHNRVETIGKWARKLQ
                     ELIPGIRIRVGHGQMEPEELEQVMLDFVEGRADILLSTTIIESGLDIPRANTMFVDRA
                     DTFGLSQLYQMRGRIGRSTQRAFCYLLIPPEANMTADAKQRLHVLQRFSDLGSGFNIA
                     SHDLEIRGAGDLLGTRQSGTIAAVGFEMYTRMLEEAVAELRGQPLERPLDPDLTCDLL
                     AYIPEEYLPDTGQRLDFYRKLGLAEDEEQVSDVLTELSDRYGALPDEVKCLGELMVVK
                     ALARTLRCASLELTESRLGLSLRDDTPLDPMKVAKLVSKPKSPWKLTPDQRLQRSWIG
                     DKERADRLQLAKGLLADLLTTAATG"
     gene            3952..4476
                     /locus_tag="PKL17_17060"
     CDS             3952..4476
                     /locus_tag="PKL17_17060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNK46495.1"
                     /translation="MRTVVRFALFGLLCAAFPTVGFAEEKPPEAAKPALPDLPAPPDV
                     KAPPADGEKSKTGLVSKLLAKGTGSDHPTAGCEVKVRYSGWMTDGKLFDTSEKKGAGF
                     VAQFPIDLLIKGWQEGLKLMVVGEKRRMWIPAELAYGDTPRRPGGPHGMLVFDVELVD
                     IVAAPAKPGKKGKK"
     gene            4483..5364
                     /locus_tag="PKL17_17065"
     CDS             4483..5364
                     /locus_tag="PKL17_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46496.1"
                     /translation="MMPSRLGLRLVLLVLWLGCNRIDYPSGPLPTAPPLPPPGEPRSR
                     ALDGRSVLLFLDQQRLIPLYCHDHGRRLFGADCAKLLPSGGNLDVRISSGAVGTIGEI
                     GNSRCTLAGAPLPSYAMMVTDAGLFGATAALWSDTSYPLVSAPPEPPEIQPQTPSQRR
                     GMRAACLQLGQALGGIPAQAEPTATWNVDLDGDGVRERLDEVRCKHATTGADIGQQLL
                     LTAGRRPERTVPIRAATDPSTLMQPVAVADVDSNGVPELVLRTVSPAKLRIELARLES
                     AGLVSLVDVECLPPARR"
     gene            5485..5757
                     /locus_tag="PKL17_17070"
     CDS             5485..5757
                     /locus_tag="PKL17_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46497.1"
                     /translation="MKPTVHALLVSGLFFGLFSATACTETSKDWVRSEKPLSDVDRAS
                     LQCQTWAEASAIHTGDEEKTKGNVKTLFQRCMKLNGYKEQETVEYK"
     gene            5788..7251
                     /locus_tag="PKL17_17075"
     CDS             5788..7251
                     /locus_tag="PKL17_17075"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNK46498.1"
                     /translation="MKHTPLAIFGTSSHAGKTTVTAGLCRLFARRGLRVAPLKSQNMS
                     LNSYATEAGEEIARSTAVQARAAKQIPTVAMNPILLKPKADDVSQLILLGKPYADVTA
                     AEYFGSDKLQSLKWQAIDAAIASLRRDYDLIVVEGAGSCAEPNLRALDVVNFGLAARL
                     HADVHIVTDIDKGGVFADILGTLNVLSLTEPSDAARVRGFLINKFRGDPKILDGGLDF
                     IRPHIGSAQLAVLPYLDLELEEEDRIRPRHCPNPEVDIAVVYLPHIANATDFEYLAVE
                     HNVSLRFVRHAAELGAPDAIILPGSKNTSWDLDYLRRCGLADALLSQQGKTPLFGICG
                     GFEMLGGTLFDPDQTESKLGSLPGLGLLAVDFVFQRHKLVRPCRYLPTEHNLLREAGP
                     IEGYEIHTGQPIYRGALPLFEGPSGLDGAIDPERMIWGTFVHDLWKNARLTRCFVDWL
                     RVRKGLPKLTVKLPDHRDRFEQSIERLADEIEALGVF"
     gene            7296..8369
                     /locus_tag="PKL17_17080"
     CDS             7296..8369
                     /locus_tag="PKL17_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46499.1"
                     /translation="MQAVSHGRADGGSAQRVEKTAVLPQPVGVRYDLAQTALSLPGGL
                     FVVGRWDGSLQLWRLTDRGPQLLFVATLLSGEGVQALAPIGEQKIVSSHDGESLALWS
                     VLGDRLERQNLVEYPKNLGIATSAAVIEVGSIRGLVVGHESGWLSFWRPSRFGLNLIT
                     TLDVRAAQPVPSPYPLKHIRGLVQTATGQLVSGSEDGDLVLIKLGSSLDTLHPEVGAK
                     VRYSPTAKRGINGLALWGEWLAVTNCSVGERDTNLHLWRVLPGQLLPMQSLLLRSEPQ
                     RDQVFSFAAQWSKTGTLWVTTQEGLLWQLRLVLPVPPKIPPKLGVEGKLSVASNVGTA
                     LVYDQERKLLVAVGHEATVLKVP"
     gene            8370..9293
                     /locus_tag="PKL17_17085"
     CDS             8370..9293
                     /locus_tag="PKL17_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNK46500.1"
                     /translation="MTTRTWRRLLDTVRFRSVMLVGLLLFAWLPCSRAAYPVTVSDGQ
                     GRPVVLAKKPQRIVVLGPALYAYMLVDLGAADRVVGITQSADNPLEMQSLPSLGQPLS
                     PDLERIVKLAPDLVLGGTDTVQKQLLSLSIPCYAGGRAGAGIVSSQDVFALVRAVGQL
                     VFGDDVAAEKLIEKTRAEMSFVESKLAGAARSRVAVLYAGPRGNLYAAGAETPEDELV
                     LRSVGENVFGHLRHHKPVSIEELLRQQPDVILIDQSQLPLLASQSVLRSVPAVRNGQV
                     FGINAAVHGSSRLGLALRTYAQRIHPRAFGP"
     gene            9303..>9617
                     /locus_tag="PKL17_17090"
     CDS             9303..>9617
                     /locus_tag="PKL17_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNK46501.1"
                     /translation="MPGRATGLWIALALFVISSVVLSCLIGSVHVPLGAVVAGLAQRL
                     SGSSQPESPLVTIFFDLRLPRVLIGLLCGAALSLSGGVMQGVFRNPLADPYVLGTVSG
                     ATF"
BASE COUNT         1727 a         2842 c         3099 g         1949 t
ORIGIN      
        1 gattgtcagc aagctggcgc cgccgcgctg ggtcggcgtg atgattggtg tgtggctttt
       61 gtcgacggcg agtggaaatc ttctcgtcgg tctgctcggt cgctattggg aacagtggcc
      121 gcacagccaa tacttcgcgg tgatcgcggc gggacttttg gtgtcggcgc tgctgctcct
      181 ttcgcagctg cgctggcttc gtcgtgttat cccgtcgcag ccgtcgtgag caagtcggcc
      241 aaaagaccct tggcgagctg caagcggtcg gcgcgttctt tgtcgccgat ccacgatcgc
      301 tggagccgct gatcgggcgt cagtttccac ggcgacttgg gcttgctgac cagcttcgcg
      361 accttcatcg gatcaagcgg cgtgtcgtcg cgcaggctca gcccaagccg tgattcggtc
      421 agttccagcg acgcgcagcg cagggttcgt gccagcgctt tgacgaccat cagctcgccg
      481 aggcacttga cctcgtcggg aagcgcgccg tagcggtccg aaagctcggt gagcacatcg
      541 gaaacctgct cttcgtcctc ggcaagcccc agcttgcgat agaagtcgag ccgctgaccg
      601 gtgtcgggca gatattcctc gggaatgtag gccaacaggt cgcacgtcaa atcgggatcg
      661 agaggccgct ccagcggctg accgcgcagc tctgcgacgg cttcttcgag catgcgggta
      721 tacatctcga aaccgaccgc cgcgatggtg cctgactgcc gtgtcccgag gagatcgccc
      781 gcaccgcgaa tttccagatc gtggctggcg atgttaaatc ctgaccccaa atcagaaaac
      841 cgctgcagca catgcagtcg ctgtttggcg tcggcagtca tgttggcttc cggcgggatg
      901 agcaggtaac aaaacgcgcg ctgcgtcgat cgaccgatgc gaccgcgcat ttgataaagc
      961 tgcgacagac caaacgtatc ggcgcggtcg acgaacatcg tgttggcgcg cggaatgtcg
     1021 agcccgcttt cgatgatcgt cgtcgatagc aaaatgtcgg cgcgtccctc gacaaagtcg
     1081 agcatcacct gctctagttc ctcgggttcc atctggccgt ggccgacgcg aatgcggatg
     1141 ccgggaatca gctcttgcag cttgcgcgcc cacttgccga tcgtctcgac gcggttgtgg
     1201 acaaaaaaca cctgaccgcc gcgtcgcagc tcccgctcga tgccgtcgcg aatcagatca
     1261 tccgattccc gcgcgacgat ggttcggatc gccaaacgat ctgcgggtgc cgtcgaaatg
     1321 atcgaaattt cgcgaatgcc cgacagcgcc atgtgcagcg tgcggggaat cggcgtcgcc
     1381 gtcagcgtca gcgtatcgac ttgcgaacga aactgccgca gccgttcctt gtgcttgacg
     1441 ccaaagcgct gttcttcgtc gacgatcacc aggcccaggt ctttgaagcg cacgtccgtc
     1501 gacaaaagcc gatgggtgcc aacgatgata tcgagttttc cgtcggctaa gtcttttagc
     1561 tctttgagct gctcggcccg cgaacggaag cgcgacagcg acgacaccgt aatcggcagc
     1621 gtcttcatcc gattggaaaa cgtcgtcgcg tgttgttcgg taagcacggt cgtcggcgcc
     1681 agcaccgcca cttgcttgcc ggcgagcacg gctttgaccg cggcccgcat cgcaacttcg
     1741 gtcttgccgt agccgacgtc gccgcagacc aagcgatcca tgggccgttc cagatccatg
     1801 tcgccgagca catccgaaat cgcgcgggct tgatccggcg tctcctcaaa tgggaacgtc
     1861 gcttcgaact cggcaaagag ctgatcggcg tcgctgtcga gcacaaacga gtgaccgggc
     1921 agcgcctgcc gttgggcgta gattttgagc agttcctcgg cgatctgctg gacttcgcgg
     1981 atgactttgc tgcgggtttt ttgccacgtc tcgccgccca tgcggtcaag cttcggggtc
     2041 acaccctcgg caccgacgta gcgctgcact tccgaaagcc gccagaccgg cagataaagc
     2101 tgcccgccgt cgtattcgat gtgcataaag tcgatcgcga cgccgcccgt tcgcacaggc
     2161 agcttggtca ggccgcgata ccgtccgacg ccgtgcagtg gatgcacgac gtagccgccg
     2221 acttcgaggt ttttcagatc acccagcgac ggttttttgg acgctttggc ggtcttgcga
     2281 accgcttttt cgccaaaaat ttccgattcg gaataaagcg acacccgatc gagcggcaga
     2341 tcgacgccac gcagcagcgt cccgacgcgg atttcgatcc gtccgaggag ctgcggatcg
     2401 ctatcgagca gatcgaagtg atgcaccgcc tcgccacttt tttgttcgac ggcgagcgac
     2461 gacagaccgg gtcggtgcaa gctcggcttc aaaccgtagc cgcgcagaag cgattcgagc
     2521 cgctcggcgc cttgcagact gcccgcgacg atgaccgatc gcacaccgtc gtcttggttt
     2581 ttgcgcagca ccgtcgccag cggcttcagg atgtgctcgt gcttttcctt ttggccgcgc
     2641 tgaagttccg ccgccagcag ccggtgatct tcgccacaca gccgcaccgc tggaaccgcg
     2701 tcgtcttccc cggtgatgac cagcggatcg atttcgacac acaggcttgg cttggcccgg
     2761 ctaccgagca gaagctgccg cagctccgtc gggtctagat aaaattccga cggcgcaaac
     2821 gccagcttgt gatcggcgat gcgcttttga tacgccgact caccgtcgcg gtgtagctcg
     2881 ctcaggctat caaacagccg gtgtggttcg acgaccacga acgtcgaatg ctccggcaga
     2941 tattccccaa tcgacgatag cttttcgtga aacgccggaa tcagcgactc gacgccgaaa
     3001 aaatcggtcc ccgactcgat ctgttccaag atgttgcgcg tgcgcgacga cggatgcacc
     3061 gccagatcgc cgacttcaag cagccgctcg cgcagcttgt ggccgcgcgt caggatcgtc
     3121 tcgcgcaccg ggtggatgta aatttcgtcg gttttgcgca gcgtgcgctg cgaggtcgga
     3181 tcgtaaaaac gcagcgactc gacgagatcg ccgaaaaatt cgatccgcac cgggaagcga
     3241 tacagcggca caaacacgtc gagaaccccg ccgcgcaccg cataggttcc ggggtcttca
     3301 cagacgggag ctcgggtgta tccgccttgc gccagtcgct tgatggtttg atcgcgatcc
     3361 agctcctctt ccgcagcgat gatgtcgacg aagtctgcaa acgcagcctt gggcaccact
     3421 ttgcgcgaca gcgcttcgac ggaagcgacc acgacttgcg gcggcagcac gctggcttgt
     3481 gacagccgaa acagcgtcga catacgcgac aaaaccgcag cccgctcggg cgaggcatcg
     3541 gcccacggcg tcacatccag ttcgggcagt ccaatcaccg catcggaagc tgctggatcg
     3601 tcggtgcttt ggcctcgatg caagaaaaac tgtaggtctt tgcgcagcgt gcgcgccgcg
     3661 ttttcatcgg cgaccagcgc gaccagcggc agcgattttt gcggcagctt gtcgaccagt
     3721 ttggcgatga gcagcgcttg caaaggcccc acggtcccaa acgtgcgcag ccgggtctcg
     3781 ccggcactta tccgcgacaa aagacgcggc agtgcggcgc gcagcggcga tacgggcagc
     3841 tcgtctaagc tgactggttc tgcgtgatcc tcgacgtgat ccgggtgccg atccatggcg
     3901 cggcgttata ccgcattgcc ccgcagagag tcggcaaagt aaagtgacct catgcgtaca
     3961 gtcgtccgct ttgcgctttt cggtctgctc tgtgctgctt ttcccacggt tggctttgcc
     4021 gaggagaaac ctcccgaagc ggccaagccg gcgctacccg acctgccggc cccgcccgat
     4081 gtcaaagccc cgcccgccga cggtgaaaag agcaaaaccg gcctcgtctc gaagctgctt
     4141 gccaaaggca ctggcagcga ccatccgacc gccggctgcg aggtcaaggt ccgctactct
     4201 ggctggatga ccgacggcaa gttgttcgac acgtcggaaa aaaaaggcgc cggctttgtg
     4261 gcccagttcc caatcgacct gcttatcaaa ggctggcagg aaggtctgaa gctgatggtc
     4321 gtcggcgaaa agcgtcggat gtggattccg gcagagctgg cctacgggga cacgccgcgt
     4381 cgtcccggtg gtccgcacgg catgctggtg ttcgatgtcg aactggtcga tatcgtcgct
     4441 gcacctgcca agccgggcaa aaaaggcaaa aagtagccag cgatgatgcc ttctcggttg
     4501 gggctgcgcc ttgtgctgtt ggtgctttgg ctcggctgca atcggattga ctatccaagc
     4561 ggcccgctgc cgacagcacc gccgctgccg ccaccgggtg aaccgcggag tcgggcgctc
     4621 gacgggcgat cggtgctgct gtttttggac cagcagcgtc tgatcccgct ctattgtcac
     4681 gatcacgggc ggcggctttt tggggccgac tgtgccaagc tgttgccgtc gggcggaaac
     4741 ctggatgtgc ggatctcgag cggtgccgtc ggtacaatcg gggaaatcgg caacagtcgc
     4801 tgcacgctgg ccggggcgcc tttgccgtcc tatgcgatga tggtgaccga cgcggggctt
     4861 tttggcgcga cggctgcgct gtggtcggat acgtcgtatc cgcttgtgtc ggcgccaccg
     4921 gaaccgccgg aaattcagcc gcaaaccccg agccagcgac gaggtatgcg ggctgcttgc
     4981 cttcagcttg ggcaggcgct cggcggaatc ccagcccaag ccgaaccgac ggcgacctgg
     5041 aacgtcgatc tcgatggcga tggcgtgcgc gaacggctcg acgaagtgcg ctgcaaacac
     5101 gccaccacgg gcgcggacat cggtcagcag ctgctgttga ccgccggacg tcgtcccgaa
     5161 cgaacggtgc cgatccgcgc cgcaaccgat ccttctacgc tgatgcagcc cgtcgctgtt
     5221 gccgatgtcg attccaacgg cgtgccggaa ctggtgcttc gcacggtgtc tccggccaag
     5281 cttcgcattg agctggctcg tctggagagc gcaggtcttg tgtcgctcgt cgacgttgag
     5341 tgtctgccgc ctgcccgtcg ctagcgtcct tatgcgtcgc tcgggatgtc cccgtgcggc
     5401 tggcacagcg cgccaaacgc cttgaaactc gcggcggatt gggcaaaagt tcacccaaac
     5461 cacagccgat tcgagagaac gcgcatgaag ccgactgttc acgcactgct agtctcggga
     5521 ctttttttcg gtcttttttc cgccaccgcc tgcaccgaaa cgtcgaaaga ctgggttcga
     5581 agcgaaaaac cgctgagcga tgtcgaccgc gcctcgcttc agtgtcagac gtgggccgaa
     5641 gcctcggcga tccatactgg cgacgaggaa aaaaccaagg gcaacgtcaa gacactgttt
     5701 cagcgctgca tgaaactgaa cggctataaa gagcaagaga ccgtcgagta caagtagttt
     5761 tctcgtcgtg tacaagaggc tcgctgtttg aaacacacgc cgcttgcgat ttttggtacg
     5821 agctctcacg ccggcaaaac gacggtgaca gcggggctgt gtcggctgtt tgcgcgccgt
     5881 ggactgcggg tcgcgccgct caagtcgcag aacatgtcgc tgaattcgta tgcgacggaa
     5941 gcgggggaag aaatcgcgcg ctcgacggcg gtgcaggcgc gtgcggccaa gcagataccg
     6001 acggtggcga tgaatccgat cctgctgaag ccgaaagccg acgatgtaag tcagctgatc
     6061 ctcctcggca agccgtatgc ggacgtgacg gcggcagagt atttcggatc ggacaagctc
     6121 cagtcgctga agtggcaggc gattgatgcg gcgattgcgt cgcttcggcg cgactacgat
     6181 ctgattgtcg tcgaaggggc gggcagttgt gcagagccga atctgcgtgc gctcgacgtg
     6241 gtgaactttg gtctggcggc gcgactccac gccgacgttc acatcgtgac cgatatcgac
     6301 aaaggcggcg tgttcgcgga catccttggg acgctgaacg tgctgtcgct gaccgagccg
     6361 agcgatgcgg cgcgggtgcg tggttttctc atcaacaagt ttcgcggtga tcccaaaatc
     6421 ctcgacggtg ggcttgattt tatccgtccg cacattggct cggcccagct tgcggtgttg
     6481 ccgtacctcg accttgaact ggaagaagaa gaccggattc gaccgcgcca ctgcccaaat
     6541 cccgaggtcg acatcgccgt cgtctatctg ccgcacatcg ccaacgcgac tgattttgaa
     6601 tacctcgcgg tcgagcacaa tgtctcgctg cgctttgtcc ggcacgctgc cgagctgggc
     6661 gcgcccgacg ccatcatcct gccgggaagc aaaaacacct cctgggatct cgactatttg
     6721 cgtcgctgtg gcctggccga tgcgctgctt tcacagcaag gcaagacgcc gctttttggc
     6781 atctgcggtg gctttgaaat gcttggcggc acgctgtttg acccagatca gactgagtcc
     6841 aaactcgggt cgctgccggg gctgggtctt ttggcggtcg attttgtctt tcagcgccac
     6901 aagctggtgc gtccgtgtcg ttatcttccg acggaacaca acctgctgcg cgaggccggg
     6961 ccgatcgaag ggtacgaaat tcatactggc cagccgatct atcgcggggc gttgccgctg
     7021 tttgaagggc caagtggact cgacggagcg atcgacccgg agcgcatgat ttggggcacg
     7081 tttgttcacg atttgtggaa aaacgcccgg ctgacgcgct gctttgttga ctggctgcgc
     7141 gtgcgcaaag gtctaccgaa actgaccgtc aagctgcccg atcatcgcga tcgttttgaa
     7201 cagagcatcg agcgactcgc cgacgaaatc gaggcgctgg gggtttttta gtgcgggctg
     7261 cgtttcatac ggcggctatc gtcctggccc tttccatgca agctgtttcg catgggcggg
     7321 ccgacggggg cagtgcacag cgggtcgaaa aaaccgcggt cttgccgcag ccggtcgggg
     7381 ttcgttatga cctggcgcag acggcactga gtcttcccgg tgggctgttt gtcgtcggtc
     7441 gctgggatgg ctcgcttcag ctttggcggc ttaccgatcg ggggccgcag ctcttgttcg
     7501 tcgcgacgct tctgtcgggg gaaggcgtgc aggcgctggc cccgattggg gaacagaaga
     7561 tcgtgtcaag ccacgacggg gaatcgctgg cgctgtggtc tgtgctgggc gatcggctgg
     7621 aacgacaaaa cctggtcgaa tatcccaaaa atttgggcat cgcgacgagc gcagcggtga
     7681 tagaagtcgg ctcgatccgc ggtctggtgg tggggcacga gtcgggctgg ctgtcgtttt
     7741 ggcggccttc gcggtttgga ctgaatctga tcacgacgct cgatgtgcgg gcagcgcagc
     7801 cggtgccaag tccctatccg ctcaagcaca ttcgtgggct tgtgcagacg gcaacgggac
     7861 agctggtgag cggttcggaa gacggcgatt tggtgctgat caagctggga tcatcgctcg
     7921 acacgctgca tccggaagtc ggcgcgaaag ttagatacag tccaacagca aagcgcggca
     7981 tcaatggctt ggcgctgtgg ggcgaatggc tggcggtgac aaactgttcc gtcggtgagc
     8041 gagacaccaa cttgcacctg tggcgggtgc tgccggggca gcttctgccg atgcagtccc
     8101 tgcttttgcg cagcgaaccc cagcgcgatc aggtcttttc gtttgcggca cagtggtcaa
     8161 agacgggcac gctgtgggtg acgacgcagg aagggctgct gtggcagctt cggctcgttt
     8221 tgcccgtccc gccgaagatc ccgccgaagc tgggagtcga aggaaagctg agcgtcgcca
     8281 gcaacgtcgg gaccgcgtta gtctatgacc aagagcgcaa gcttctagtt gcagtggggc
     8341 atgaggcaac ggtactaaag gtgccctaaa tgacgacccg gacttggcgg cgacttttgg
     8401 acacagtgcg tttccgcagc gtgatgcttg tggggctgct gctgtttgcg tggttgccgt
     8461 gttcgcgggc ggcgtatccg gtgacggtca gcgacggcca gggccgaccc gtggtgctgg
     8521 ccaaaaaacc gcagcgaatc gtggtgttgg ggccggcttt gtacgcctac atgctggtcg
     8581 atttgggcgc ggcggatcgg gtggtgggaa tcactcagtc ggcggacaat ccgcttgaaa
     8641 tgcagtcgct cccgtcgctg gggcagccgc tgtcgcccga cttggagcgg atcgtcaagt
     8701 tggcgcctga cctggtgctg ggcgggacag acacagtcca aaagcagctc ttgtcgctgt
     8761 cgattccttg ctatgccggc gggcgagctg gggcggggat tgtatcgagc caggatgtct
     8821 ttgcgctcgt gcgtgcggtc ggtcagctgg tgtttgggga tgatgttgcg gcggaaaaat
     8881 tgattgagaa gacgcgggcc gagatgtcgt ttgttgaatc aaagctcgca ggcgcggcgc
     8941 gttcgcgggt ggcggtgctg tatgccggac cgcgaggcaa tctgtatgcc gccggggccg
     9001 aaacgccgga agacgagctg gtgctgcgca gtgtcgggga aaacgtgttt ggtcatctgc
     9061 gccatcacaa gccggtcagc atcgaagagc tgctgcggca acagcccgat gtgatcctga
     9121 tagaccaaag ccagctgcca cttttggcgt cgcagtccgt gctgcgcagc gtgccggcgg
     9181 tgcgcaacgg gcaggtcttt gggattaacg cggcggtgca tggctcgtcg cggctggggc
     9241 tggcgctgcg aacttatgca cagcgaattc acccgcgagc gtttgggcca taagctgtgc
     9301 cgatgccggg ccgagcaact gggctgtgga tcgcgcttgc cctcttcgtc atttcgtcgg
     9361 tggtgctgtc gtgcctgatt ggttcggtgc acgttccgct tggtgcagtg gtggcgggtc
     9421 ttgcgcagcg gctttctggc tcgtcgcagc cggagtcgcc gcttgtgacg atcttttttg
     9481 acctgcggct gccgcgggtg ctgatcgggc tgctgtgtgg agcggcgctg tcgctgtcgg
     9541 gcggcgtgat gcagggcgtg tttcgcaacc cactggccga cccatatgta ctcggcaccg
     9601 tcagtggagc gacgttc
//
LOCUS       DAORGN010000186         9598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3091_length_9598_cov_5.756995, whole genome shotgun sequence.
ACCESSION   DAORGN010000186 DAORGN010000000
VERSION     DAORGN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_9598_cov_5.756995"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3452)
                     /locus_tag="PKL17_17095"
     CDS             complement(<1..3452)
                     /locus_tag="PKL17_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46502.1"
                     /translation="MGTDSELKVSVPKHWTPPKAFDEYRLLWPLGRGGMGAVYLGHDM
                     LLDRAIAVKFISSLNPDAVSREQFLTEARAAARLQHPNVVTVYRVGEIDGRPYIIQEY
                     LRGESLERMKKPLPWQQVLDLAIGLSRGLAAAHRRGVLHRDLKPGNVMLPTEGGVKLL
                     DFGLAKLVDANRSQAIDVAGLVDAEKGRSGEQRAVTQSVDLLAATMAGTSTPNQRGKA
                     GGDPAKIAAEAGAAVISLSGQKTPQAKPRANPIPPPDPSDSLIDPSKLRLAAQNRLAA
                     AFGKEHTAEEIPDELWRADPLERSSTIKGTPLYMAPEVFEGDPASRQSDLYSLGAVLY
                     ELCHGKPPHYNTSLVELHRLVTTTDAPPLLSVAPSVDPRFAAVIDKCLYRDPSERYQS
                     ADDLRDALEHLLPSAQNQAIPEGNPYRGLLPFESQHRALFFGRQSEIGTLVDRLRTEP
                     FVLVAADSGVGKSSVCRAGVLPIIKEGGLGSSSSYLVATMIPGRQPLRALCDAIATTL
                     GRDEQPILAAIKTDPQTFPRVLRSMLPVGTSLLLFVDQMEEFVTIADAHEAKLVGEAL
                     GALSQKAGELRILGTVRADFVSRVATVPGIGDEINRGLYLLRPMGPDKLREAVTGPAQ
                     LKGVLFESDEMVDSLVASTAESDAGLPLLQFALAELWDVRQGSRITAKALESIGGVMG
                     ALARHADHLIGSLPAERRPLARRLLMSLVTIDGTRARRTAEELTWNDASAKSVLELLV
                     RGRLLVARETPEGTAYEVAHEALLKGWGTLRRWLEENAERRAVKHRLEQATQEWLRLH
                     KAREALWSARQMTELALLEPEDINPKEKEFVAASRAAQRRTQFFRRLALLTIPVLLIA
                     GYVGVQIAARRKLERQVSDLVRQASDVLGEASRMNAETNRLRGEAFAQFDSKHKDDGE
                     KLWAQARQAADKADKAYYRASLVLESALTLDMSRSDVSERLAEALYQRAVNADRDHQR
                     QKRDELIERMARYDKSGALQKRWTEPAKLTVATSPLGALIQVQRYVDEESGRKTLTPA
                     KELGISPIFESPLEPASYLLTITAAGKVDVRYPILLARGEQLRIDVPMPSRSEIPPGF
                     VYIPKSRFLFGYGQDETLRKVFFTTAPMHQVTISGYFIAQHETTFGDWIEYLNALPAK
                     ERKQR"
     gene            complement(3603..4421)
                     /locus_tag="PKL17_17100"
     CDS             complement(3603..4421)
                     /locus_tag="PKL17_17100"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK46503.1"
                     /translation="MRWLAGLLLFGLCSLQAPVFAQSSDAALMETLRTGKDPEARRQA
                     AVRLSKVDAVTALEPLMAALLADRSPIVRTACAEALGTIGSPAALSALRAATRDEDVG
                     VKQKAEAALARINKLAAEPVEKMVVLVSAPAGKASAKSAPAGLPQRLREQFVRELRLS
                     PDVDVRDDFKPSGSETAFSIEGTISNMSRRTTSRGELEVSYDINVVISQMPGKRVVGI
                     VTGGASTFSPKNSAGRLSKNQIDDLEQQALSQSVLAATENVLSFLRGQRKSRTY"
     gene            complement(4607..5908)
                     /gene="hutI"
                     /locus_tag="PKL17_17105"
     CDS             complement(4607..5908)
                     /gene="hutI"
                     /locus_tag="PKL17_17105"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNK46504.1"
                     /translation="MATLVLRDIGELLTMNGPAPTGSSRTEQIARAELSLGKVEDAVL
                     IVVDGLVHYAGSTNDAPPIHLLPQPVTVVTARGQLVSPGLCDPHTHLIWAGDRSREFE
                     LRNLGATYQQIAEQGGGILSTVEKTSAADDETLVEGLRRRLDTALTLGTTLCEVKTGY
                     GLWPTAELRLLRILASVAQSHVCRVSPTFLCHVPPPEAKSGEPRARLVADLAASLPTA
                     LSLGADALDVYCDQGAFTFDETRELFLAARAAGLGLRCHAEQFTHTGVAALAAAFDAL
                     SVEHLEQLDAEGRRALVESKSRRPGGTVANLLPGAALQLRLPWPDAAALFGDGVTVAL
                     GTDCNPGSSYTQSQPLMMSLACSYMGLSCAEAWIAVTVNAAQAIGRPTLGRLVPGAAA
                     DFVLWDADHYRQVCQQLGGNPVRDVWISGEPCVRQGVRLSD"
     gene            complement(5948..7420)
                     /locus_tag="PKL17_17110"
     CDS             complement(5948..7420)
                     /locus_tag="PKL17_17110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK46505.1"
                     /translation="MLRLSIRSKILLGFVVVLLTFGAATVYGAWAVHTLGQQLRLVAL
                     GYSNLRLELQDQQTIQANLLRRLLDPEREAEKGSRLRDEVAADRKLRRLRLLTTQQHV
                     DELRRNLPREDAVFLHELINTKLAELSDLYATTDAMPLSMASQGEEQASRLLQELSRL
                     LTNKVIEVVNAGSRYEARAGFLTVGLALTAALCSLVVLLLVQATLRPLRRLAESAKEV
                     ARGNYRERVPADSPDEVGMLGREFNAMAAALAEREQRLRRSEQLAAVGKMAAQITHEI
                     RNPLTSIGLNAELLADDFADGNGEAVKIAQAIVKEVDRLTDITEQYLRFARLPEPRLE
                     PVELSAMVTGLHGFLQEELGRRGVSVTLQLAESLPRVNADDNQLRQVLLNLIRNAAEA
                     MAEQPVSERTLAIATRLVTDSATMKPSVAVDVIDNGPGIPKERHRQIFEPFFTTKKGG
                     TGLGLALSLEVISRHGGKIHLQSPLTSGRGTQFTVELPAT"
     gene            7505..8734
                     /locus_tag="PKL17_17115"
     CDS             7505..8734
                     /locus_tag="PKL17_17115"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46506.1"
                     /translation="MRSRRSYGISSAWLLPSLFLGFVAVAQAAPTRVGLLGVDGSAVE
                     PGMGAILTDALRRNLPQLHGMRIEPRSQDLAEVKMVFGCGDERPECMTKVGRNLEVAR
                     LIYGSIRKQAGGYVVLIRQLNVADGTVEKTLSETVPKQILMEPSARLDEIIQGWLRQL
                     LVEGLRGDLVVSSNPPGALVYVDGEPAGRTPHTAAGLDVGNHVLKIELTGYDPIVKTV
                     QIRGNLSETVDTQLHAQTGAATAPSTTKPASRTNWTPLLRYTSYALYGLAGVTAIAAL
                     GTWGAKNKAEDQANAQIDRLIADLGPRAVDYASFFHSQASLSRCAGPDSLVGNAAYDA
                     YADACRSGNRLAGAASGLWIAAGTFAVVGAATMLSTYFLKKDAEAATATADKPLNLRV
                     APSVVPSGAMVHAAFDF"
     gene            8715..9320
                     /locus_tag="PKL17_17120"
     CDS             8715..9320
                     /locus_tag="PKL17_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46507.1"
                     /translation="MRHSTSNSAQRNGHRPNARLAEPTPGKLVGWRSAGLFGLIAIGL
                     LGGCVTGKAHPEKKIEPIGEAELAAKKLQDPLDKHLCKAAIHPIAKADWKLTRVASLI
                     GRGPNFQAALEALCREADGQKFPAIVDIYYERAVTAWSPNHAVRGTAVRFGDGVDPPA
                     PPELAAISPPPMPKATDDEPLPAGEAVPPPPPPNPTARSGK"
BASE COUNT         1813 a         3023 c         2900 g         1862 t
ORIGIN      
        1 cgttgttttc tctcttttgc cggaagcgca tttagatact caatccaatc tccaaatgtt
       61 gtctcatgct gcgcaatgaa gtagccagaa atggtcactt ggtgcattgg cgcggttgtg
      121 aaaaagacct tccggagggt ttcgtcctgg ccgtagccga agaggaagcg gcttttgggg
      181 atgtagacga aaccgggggg aatctcgctg cgcgacggca tcggtacgtc gattcgcagc
      241 tgttcgccgc gagcgagcaa aatcggatag cgcacatcca cttttcccgc cgcggtgatc
      301 gtcagcagat acgaagccgg ttcgagcggc gactcgaaaa tcggcgaaat gcccagctct
      361 ttggctgggg tcagcgtttt gcggccactt tcttcgtcga cgtagcgctg aacttgtata
      421 agcgccccga gcggactcgt cgccaccgtg agcttggcgg gctcggtcca gcgtttttgc
      481 agggcaccgc ttttgtcgta gcgggccatg cgctcgatca gttcatcgcg cttctggcgt
      541 tgatgatccc gatcggcatt gacggcgcgc tgatacagcg cctcggcaag acgctcgctg
      601 acgtcgctgc gactcatgtc gagcgtcagc gccgactcga gaaccagcga ggcgcggtag
      661 tacgctttgt cggctttgtc cgctgcttga cgagcctgcg cccacagctt ttccccatcg
      721 tctttgtgct tggaatcgaa ctgggcaaac gcttcgccgc gcagtcgatt ggtctcggca
      781 ttcattcgag acgcctcgcc gagcacatcg ctggcctggc gaaccaaatc gctgacttga
      841 cgttcgagct tgcgacgggc cgcaatctgc acaccgacat atccggcaat cagcagcacc
      901 gggatggtga gcagcgccag ccgccggaaa aactgggttc gtcgctgtgc cgcgcgcgag
      961 gccgcaacaa actctttttc tttgggattt atgtcttcgg gctcgaggag cgcaagctcg
     1021 gtcatctgcc gggccgacca cagcgcctcc ctcgctttgt gaagacggag ccactcctgg
     1081 gttgcctgtt ctagcctgtg cttgacggcg cggcgttcgg cattttcttc gagccagcgt
     1141 cgcagcgtcc cccagccttt gagcagtgct tcgtgcgcca cttcataggc ggttccctcg
     1201 ggcgtttcgc gcgcaaccag cagacggcca cgcaccagca gttcgagcac gctcttggcg
     1261 ctcgcgtcgt tccaggtaag ctcctcggcg gtgcgacggg cgcgggtgcc gtcgatggtg
     1321 accagcgaca tcagcagtcg acgcgccagc ggtcgtcgct cagccggcag cgaaccgatc
     1381 aggtgatcgg cgtgccgagc cagcgcaccc atgacaccac cgatcgattc cagtgctttg
     1441 gcggtgatcc ggctgccttg acgaacgtcc cacagctccg cgagcgcaaa ctgcaaaagc
     1501 ggcagtcctg cgtcgctctc tgccgtcgat gcgaccaatg aatcgaccat ttcgtcgctt
     1561 tcgaacagca cacctttgag ctgcgccggc cccgtgaccg cctcgcgcag tttgtccggg
     1621 cccatcggtc gcagaagata aagtccgcga tttatctcgt caccgatccc cggcaccgtc
     1681 gccacgcgcg aaacaaagtc cgctcgtacc gtgcccaaaa tgcgaagctc accagctttt
     1741 tgcgaaagcg ccccgagcgc ttcaccgacg agctttgctt cgtgcgcatc ggctatcgtc
     1801 acaaactctt ccatttgatc gacaaacaga agcaggctcg ttccgacggg cagcatgcta
     1861 cgcagcaccc gcgggaacgt ctggggatcc gtcttgatcg cggccaaaat cggctgttca
     1921 tcgcggccta gtgtcgtcgc gatcgcatcg cagagcgcgc gcagcggctg gcgtcccggt
     1981 atcatcgtcg cgacgagata gctcgaactg ctacccagtc cgccttcttt gatgatcggc
     2041 agcacgcccg cgcgacacac cgacgattta ccgacaccag agtcggcagc gacgagtaca
     2101 aatggttcgg tgcgcagccg atcgacgagc gttccgattt cggactgtcg accaaaaaac
     2161 agcgcgcggt gctgcgactc gaacggcagc agtcctcgat acgggttgcc ttccggaatc
     2221 gcttggtttt gcgccgatgg cagcaggtgt tccagcgcat cccggagatc gtcggcactt
     2281 tgatagcgct cactcggatc gcgatacaga cacttgtcga tcactgctgc gaagcgtgga
     2341 tcaaccgacg gggcgacgct cagcagcggt ggcgcatcgg tcgtcgtcac caggcggtgt
     2401 agctcgacga gcgatgtgtt gtagtgcggc ggcttgccat ggcaaagctc gtacaaaacc
     2461 gcgccgagag aatagagatc ggactgcctc gacgctggat ctccttcaaa cacctcgggc
     2521 gccatgtaga gcggcgtgcc tttgatcgtg ctcgatcgct caagtgggtc cgcgcgccac
     2581 agttcgtcgg ggatttcttc cgcggtgtgt tctttgccga acgctgcggc cagtcgattc
     2641 tgtgccgcga gtcgcagctt gcttggatcg atcagcgagt cactcggatc gggcggcggg
     2701 atcgggttgg cgcgcggctt cgcctgcggc gtcttctgtc ccgacagaga aatcaccgca
     2761 gcacctgctt cggcggcgat cttggctggg tcgccacccg ctttgcctcg ctgattgggc
     2821 gtgcttgtgc cagccattgt cgcggcgagc aggtcaaccg actgcgtgac ggctcgctgt
     2881 tcgccggatc gacctttttc cgcatcgacc aggccagcca cgtcgatcgc ttgcgatcga
     2941 tttgcgtcaa cgagcttggc caagccgaag tcgagcagct tgacgccacc ctccgtcggt
     3001 agcatgacgt taccgggctt cagatcgcga tgcagcacac cgcgccgatg ggcagccgcc
     3061 agaccccgcg acagtccgat cgcaagatcg aggacttgct gccagggcag cggctttttc
     3121 atccgctcca ggctttcgcc gcggagatat tcctgaataa tgtacggtcg tccgtcgatc
     3181 tcaccgacgc ggtagaccgt cacgacattg gggtgttgca gccgagccgc agccctggct
     3241 tcagtcaaaa actgttcgcg cgacaccgca tcgggattca gcgaagagat gaacttcacc
     3301 gcgatggcgc gatcgagcag catgtcgtgg cccagataga ccgcacccat tccgccgcga
     3361 ccgagtggcc agagcaggcg atactcgtcg aatgcttttg gcggagtcca gtgctttggc
     3421 acgctgactt tgagttcgga gtccgtcccc atgtcctgca cttgcgcgag gtactgatct
     3481 tcaaccgtcg cattttggcg acggtcgcgt caacccccaa gacttccacc gctttcctcg
     3541 gctagagctt ggttttgtcg acgaaatggt agcaagggga gtgccggcct tcccctgcaa
     3601 gactagtagg tgcggctctt ccgttgtccg cgcagaaacg aaaggacatt ttcagtcgcc
     3661 gcgaggaccg actggctgag cgcttgctgt tccagatcgt cgatctgatt tttgctaagc
     3721 cgcccggcgg aatttttggg cgaaaacgtg ctggcgccgc ccgtgacaat gccgacgacg
     3781 cgctttccgg gcatctgcga gatcacgacg ttgatgtcgt aggacacttc gagctcgccg
     3841 cgtgacgtcg tgcggcgcga catgttgctg atggtgcctt cgattgaaaa ggcggtctcg
     3901 ctgccgcttg gtttgaagtc gtcgcgcaca tcgacgtcgg ggcttaggcg cagctcccgt
     3961 acaaactgtt cacggagtct ctgcggtaga cctgccggcg cggacttcgc cgatgctttg
     4021 cccgctggcg cgctcaccag caccaccatt ttttccaccg gctccgccgc cagcttgttg
     4081 attcgcgcaa gtgcggcttc ggctttctgt ttcaccccga catcttcgtc acgggttgcc
     4141 gcgcgcaggg ctgaaagtgc cgcgggtgaa ccgatcgtgc ccagtgcttc cgcacacgcg
     4201 gtgcgaacga tcggactgcg atcggcaagc agcgcggcca tcagtggttc gagcgccgtc
     4261 actgcatcga ccttgctaag tcgcaccgca gcttggcgtc tcgcttcagg gtctttgccg
     4321 gtgcgcagcg tttccatcag ggccgcgtcg ctgctttggg caaagaccgg cgcctgtagg
     4381 ctgcacagac cgaacagaag cagcccggcc agccagcgca taggcgacgg caaccgagcg
     4441 agcagcaact ggaattgggc ggtttgtcgt cgatgcaagt gtggccccac agcgaggtcc
     4501 gaattagatg cagcgcggat cgatacgtca agcggcttgg cgatccgccg atgcaacccc
     4561 tgtgaacctg tccacgcctc aagcattgac cgcatcaccc tgatcgctag tcggacaggc
     4621 gcacaccttg gcgcacacaa ggctcgccgg atatccagac atcgcggacg ggattgccac
     4681 ccagctgctg acacacttgt cgatagtgat cggcatccca cagcacaaaa tcggcggcgg
     4741 cgcccggcac aagccgtccg agcgtaggtc gtccgatcgc ttgcgccgca ttcacggtga
     4801 cggcaatcca agcctctgcg catgacaaac ccatgtacga gcaggcgagc gacatcatga
     4861 gtggctgcga ctgcgtgtac gagctgcccg gattgcagtc ggtgccaagt gccaccgtca
     4921 cgccatcgcc aaaaagcgcc gccgcatccg gccaaggcag tcgcagctga agcgctgccc
     4981 ctggcaacag gttggcgacc gtgccgccag gacgacgtga cttgctttcg accagcgcgc
     5041 ggcgaccctc ggcatccagc tgttccagat gttcgaccga gagtgcatcg aacgctgcgg
     5101 cgagcgcggc cactccggtg tgggtgaact gctcggcatg gcagcgcaga cccaggccgg
     5161 cggcgcgtgc ggccagaaac agctcgcgtg tctcatcgaa ggtgaatgca ccctgatcgc
     5221 agtagacatc gagcgcgtcg gcaccaagcg acagagcagt gggcagactg gctgcaagat
     5281 cggcgaccag acgggcgcgc ggttcaccgg atttcgcttc cggcggcggc acatgacaca
     5341 aaaacgtcgg tgaaacccga cagacgtggc tttgcgcaac gctggcaagg attcgcagaa
     5401 gccgcagctc cgccgttggc cacaagccgt aacctgtctt gacctcgcac agcgtcgtgc
     5461 ccagcgtcag cgccgtatcg agccgccgcc gcagaccttc gaccagcgtt tcatcgtcag
     5521 cggcgctggt tttttccacc gtcgataaaa tgccgccgcc ctgttcagcg atctgttgat
     5581 acgtcgcccc gaggttgcgc agctcaaatt cccggctgcg atcgcctgcc caaatcaagt
     5641 gggtgtgcgg atcgcaaaga ccgggcgaca caagctgtcc gcgcgcggtc acgactgtca
     5701 ccggctgcgg cagcagatga atcggcggcg catcgtttgt cgatcccgcg tagtggacca
     5761 gaccatcgac cacgatcaac accgcatctt cgactttgcc tagcgatagc tcggcgcggg
     5821 cgatttgttc ggtgcgcgac gatcccgtgg gtgccggccc gttcatcgtc agaagctcgc
     5881 caatgtcacg cagcacaagt gtcgccatgg ccatgacgat gcaatgcgaa agggcaaaca
     5941 ggcaacctca agttgcgggc agctcgacgg tgaactgcgt accacgaccg ctcgtgagcg
     6001 gcgactgaag gtgaattttc ccaccgtggc gcgagatcac ttccaggctg agcgccagac
     6061 ccagcccggt gccgcctttt ttggtcgtaa aaaacggctc gaagatttga cggtggcgtt
     6121 ctttgggaat gcccggtcca ttgtcgatca cgtcgaccgc taccgatggc ttcatcgtcg
     6181 ctgaatcagt cacaaggcgg gttgcgatcg ccaacgttcg ctcgctcacc ggctgttcgg
     6241 ccatcgcctc ggcggcgttg cgaatcaggt tgagcagcac ttgccgaagc tggttgtcat
     6301 cggcgttcac ccgaggaagc gattcggcaa gctgaagcgt caccgacacc ccgcgccgac
     6361 ccagctcttc ttgcaaaaag ccgtggagcc ccgtcaccat cgcgctaagc tccaccggtt
     6421 cgagccgcgg ctctggcagc cgggcaaagc gcaggtactg ttcggtgatg tcggtcagcc
     6481 gatcgacttc ttttacgatc gcctgggcaa tttttaccgc ttcgccattt ccatcggcaa
     6541 agtcatcggc caaaagctcg gcattgagcc cgatcgacgt gagcggattg cgaatttcat
     6601 gggtgatttg ggccgccatt ttcccaaccg ctgcaagctg ttctgatcgc cgcagccgct
     6661 gctcgcgttc ggccagcgcc gccgccattg cattgaactc ccgtcccagc atgcccactt
     6721 catccggcga atcggcgggt acgcgctctc gatagttgcc gcgggcgact tccttggcgc
     6781 tttccgccag ccgtcgcaaa ggtcgcagcg tcgcctgcac cagcagcagc accaccaaac
     6841 tacacagcgc cgccgtcagc gcgagcccga cggtcagaaa gcccgccctc gcttcatatc
     6901 gtgagcccgc attcacgacc tctatcacct tgttggtgag cagccgcgaa agttcctgca
     6961 aaagccgcga cgcttgctct tcgccttgtg acgccatcga cagtggcatg gcatcggtcg
     7021 tcgcatacag atcggaaagc tctgccagct tggtgttgat cagctcgtgc agaaacactg
     7081 cgtcttcccg cgggaggttt cgccgcagct cgtcgacatg ctgctgggtc gtaaggagcc
     7141 gcagccgtcg cagcttgcga tctgccgcca cttcatcgcg caggcgtgaa cctttttccg
     7201 cttcacgttc tggatcgagc aggcgccgta gcaggttggc ctggatcgtc tgctgatctt
     7261 gcagttccag acgcaagttc gaatagccaa gcgcgaccag ccgaagctgc tgcccgagcg
     7321 tatgcaccgc ccacgcgcca tacaccgtgg ccgctccaaa tgtcaggagc accaccacaa
     7381 agccaagcag aatcttgctg cggatcgaca ggcgaagcac gcaccgcccc ttgcatggga
     7441 aaaattgtga aggactcagg atataatcca gcgggagaca aaattgcgaa ccgagtcgat
     7501 gcctttgcgg agcaggcgct cctacgggat ttcttcggcc tggctgctgc cctcgctctt
     7561 tttggggttt gtggcggttg cgcaggcggc gcccacgcgg gtcggtctgc tcggggtcga
     7621 tggttccgcc gtcgagccgg gcatgggggc aatcctgacc gatgccctgc gtcgcaattt
     7681 gccgcagctt cacggcatgc gcattgagcc gcgcagccaa gacctcgccg aagtgaaaat
     7741 ggtgttcggt tgcggcgacg agcggcccga atgcatgacc aaagtggggc gcaatctgga
     7801 ggtcgcgcgg ctcatctatg ggtcgattcg caagcaggcg ggcggctacg tcgtgctgat
     7861 tcggcagctc aatgtcgccg atggaacggt cgaaaagaca ttgagcgaga cggtgcccaa
     7921 gcaaatcttg atggaaccga gcgcgcggct cgatgaaatc atccaaggtt ggctccgcca
     7981 gctgcttgtc gaaggcctgc gcggcgatct ggtggtcagc agcaatccgc ccggcgcgct
     8041 ggtctacgtc gacggtgagc cagcgggtcg gacgccgcat accgcagccg ggctcgatgt
     8101 cggaaaccat gtgctgaaaa tcgagctgac cggctacgac cccatcgtaa aaaccgtgca
     8161 gattcgcggc aacttgagcg aaacggtcga cacgcagctt cacgcgcaga ccggcgcggc
     8221 cacagcgcca tccaccacca agccagcgtc gcggacaaac tggactccgc tgctgcgcta
     8281 cacgtcgtat gcgctctatg gtctggcggg cgtgaccgcg atcgccgcgc tgggaacgtg
     8341 gggggccaaa aacaaggccg aagatcaagc gaacgcgcag atcgaccgcc tgatcgctga
     8401 ccttggaccg cgggccgtcg actacgcgag cttctttcac agccaagcca gcctgtcgcg
     8461 ctgcgccgga cctgattctc tcgtcggcaa tgcggcctat gacgcctatg cggatgcgtg
     8521 tcgcagcggc aatcggctcg caggtgcggc aagcgggcta tggatcgcgg cgggaacctt
     8581 cgccgtcgtc ggtgccgcca caatgctgag cacgtacttt ctcaagaaag acgccgaggc
     8641 tgcgacagcg accgctgaca agcctctcaa cctgcgggtc gcaccttcag tcgtcccgtc
     8701 tggagccatg gttcatgcgg cattcgactt ctaattcggc ccagcgaaac ggacatcggc
     8761 caaacgcccg cttggcggaa ccgacaccgg gaaagctggt gggatggcga tcggctgggc
     8821 tttttggcct gatcgcgatc gggctgctcg gtggctgcgt gaccggcaag gcgcacccag
     8881 aaaagaagat cgagccgatc ggcgaggcgg agctggctgc caagaaactg caagatcctc
     8941 tcgacaaaca cctgtgcaaa gccgcgatcc atccgatcgc caaagctgac tggaagctta
     9001 cgcgggtggc atcgctgatc ggtcgcggcc ccaattttca ggcggcgctc gaggcactgt
     9061 gtcgcgaggc tgacggccag aaatttcccg cgatcgtcga tatctattac gagcgggcgg
     9121 tcacagcctg gtcgccaaat catgcagtgc gcggaactgc tgttcgcttt ggcgacggcg
     9181 ttgacccacc tgcgccacca gagcttgcag cgatcagccc cccgcccatg cccaaagcca
     9241 ccgacgatga accgctgcct gccggggagg ctgttccgcc gcctccaccc cccaacccaa
     9301 ccgcgcgatc agggaaataa aaggcgatcg gcaggcccga tcggcccgta caagccccca
     9361 caagccctgc tgatctgctg ccgtgaacga ttgtttccga cggaagtgca gattttcgaa
     9421 aaaaagatgt tgaccctttc ggatgccttg gggtagataa cttctcactg gcggcgctga
     9481 agagccaacg gcaaggccga acgaaagttt ggtagttgct tgttggttcg ttttgcgcgc
     9541 tggaaaagtt tggtccttga aaactgaata gcgagaagca gggaaaaata catacatg
//
LOCUS       DAORGN010000187         9589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3099_length_9589_cov_6.436438, whole genome shotgun sequence.
ACCESSION   DAORGN010000187 DAORGN010000000
VERSION     DAORGN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_9589_cov_6.436438"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            501..1202
                     /locus_tag="PKL17_17125"
     CDS             501..1202
                     /locus_tag="PKL17_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46508.1"
                     /translation="MSPKSTTSTVFVQLAGVALLLVGTIGDATAAPKYFIKVRDTELA
                     AGIDASVPQHGTPAASPGAQDKPDAGTGSAAPAPQDGPKTAADMVKNVLTETLRKQPE
                     VTLELESPLGNPDAVKAECKRKGLRALEFAVRVLKMARTTKPPAAGKRFRTLEQVVKL
                     SVVGTTIPEGLLSVGGDGESTIQIEVGERITPGQEREVLEESVKDAVEQAVKQGMLKL
                     SQPVGKPTPEKRRKK"
     gene            complement(1245..2309)
                     /locus_tag="PKL17_17130"
     CDS             complement(1245..2309)
                     /locus_tag="PKL17_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNK46509.1"
                     /translation="MAVSRIGIDFGPSAARAAWTEGTESVLLETLTGHRFVPTVVGLD
                     KSGALHVGDSARRQLLLWPERSAGNLRRYVGTTQRLELDRKRFSATELCAVVLSQLKD
                     LAEERLGSPLHAATIAVPCHFAPEQHQAMIDAARLAGFRVAQTVLEPLAIARAQGLSA
                     SELPDGKAVMVCDIGALHVEVTLLGAEAGRFTVLSTAQCAVGSELIDEKVADHLGRAF
                     QRQNGLSVWPHRKTLARLRLAAEWAKCQLSDSELVTVRLPGLLTLADTAVDLVADLSR
                     EQLALLVEDELRRALDVIEIALRAARLRTTQLHAVLLSGGGARLPLWADLVASHAVMR
                     PLPSSAPEELIARGAALLSS"
     gene            complement(2334..2900)
                     /locus_tag="PKL17_17135"
     CDS             complement(2334..2900)
                     /locus_tag="PKL17_17135"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HNK46510.1"
                     /translation="MRPTIGSVLVCAVSAFLFGCNKPATPTAPAQASPPAPANPTISA
                     AAPAKPAESAPAPGPGKYGAPLSNAEAIAAKTVIADPQKWNDKDVKLTGKVSGVCQKK
                     GCWMTLTSGEPEAPSVRISFKDYGFFVPKDCMGKTATVEGHLMVKTIPVAEAQHYADD
                     AAKEGSPARKVTAPEHTLALVATGVEIL"
     gene            3179..4057
                     /locus_tag="PKL17_17140"
     CDS             3179..4057
                     /locus_tag="PKL17_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK46511.1"
                     /translation="MFSVTFWGVRGSIATPGADTAKVGGNTSCVEVQCGTRRIVLDTG
                     TGIRQLGQRLLGQKKAAAQPEPTDLTVFYSHLHWDHLQGLPFFAPIYSPTTKLHFYGP
                     KGVERALRTQMGDPGFPVRIDDVPARISFGELGEGMVVSLADDLIISCARLNHPGGVL
                     AYRIQYQDHALVYATDTEHYSVPDPKLVRLARDADVLIYDAQYDDAEYSGETSGVPRT
                     GWGHSTFREGIRVADAARVGQLVLFHHDPSHSDDQVAHIEATAQRIRPGTLAAREGHT
                     IAIAAAEEKPALAVAA"
     gene            4084..5661
                     /locus_tag="PKL17_17145"
     CDS             4084..5661
                     /locus_tag="PKL17_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNK46512.1"
                     /translation="MPRAPRKTATSGAEDSSRSNTVDRRSVLLALLELIRTEVDLDTL
                     LRRIVDLLAQAMDADRATLFVVDKPSGELISRAAHLPELPEIRLRLGQGIAGLVAQTQ
                     KPISLSHADDHPRFAANIDRQTGYKTVTVLAVPILCDSPTGNQELLGVIEVLNKRTGD
                     FNDDDRALLCELADEVAAALLQTRLLQRVTQNTPERYHRIIGTSTPMLRVYEIIGRAA
                     ATTATVLIRGESGTGKELAARAIHYNSQRAKGAFVKIDCTSIPEGLMESELFGHERGA
                     FTGAEQRVLGKAELADGGTLFLDEIGDLKSSLQGKLLRLLQDREFERVGGRKTLAVDV
                     RIVAATNRPLEALVESGQFRADLYYRLKVIELSMPALRERSGDDIERLAQHFVKQYAT
                     RHNKAVRGISDEALRRLRAHLWPGNIRELEHCIESAVALCPAAELSMADLPLPTASHE
                     AFLRKNPPAQPPPTDEVKPSRVDPAGLQLPFGLSLADVEKRYIQQTVERFGGNRSEAA
                     RALGIGRNTLLRKLREP"
     gene            complement(5674..7125)
                     /gene="amrB"
                     /locus_tag="PKL17_17150"
     CDS             complement(5674..7125)
                     /gene="amrB"
                     /locus_tag="PKL17_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNK46513.1"
                     /translation="MSVSSNDKEGGTQAAPGSGAAESVTQTASATVTESVSASDDPED
                     IADAAIDPSEEKPMLRPLEAFPVDGPGGQKLLALYDPSGIAPQPVTLPPLGAAVIDLC
                     DGTRNRAEILAEFAARYRRALTADALEALLKKLDDALLLDSTNFRLHCAKIYADFAQS
                     PSRPPLGAGTLYPTGVDDLIKGLESAFAAPNGPGLAVPETAGKAPRAMLLPSVEIKSG
                     GPAYAWALKPLLDAAEFPTLIVLLGCDHGAADPMLTLTRKHYQTPIGELQTDVELVDA
                     IIADATAKSTELGELVVRDEFHHRNEHSLELLAIWLRFVLDQRKKRGHKDADPKVVPV
                     LCGSLHDFAAASGSRAHEAQDTRLMDEVVHLIQQRVGEKQGAGERVLWIASADLAHVG
                     PRFGDPQEINDDDRDSLERRDRQTLKPLLMGDGQTFLAEIKRERDRRHVVGLGTIYTF
                     LQAARPTGGVLRCYAQCSVSPSSFISTASAVFP"
     gene            7632..>9589
                     /locus_tag="PKL17_17155"
     CDS             7632..>9589
                     /locus_tag="PKL17_17155"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46514.1"
                     /translation="MADQRIGNYRIVRKLGEGGMGVVFEAIHELINRRAAIKVLHADF
                     SRNPEMATRFLNEARAANIVQHPGIVNIFEFDRLPDGSAYIVMDYLDGDSLTQRLQRL
                     GGRMETGPALSLILQVAEALTAAHSKGVIHRDLKPDNIMIVPDQGDSSREQAKILDFG
                     IAKMAESQGMGPALAKTQVGVPMGTPLYMAPEQWKGASEVEDKADVYSLGVIIYEMLS
                     GQPPFIANSGSEVMALHILATPRPLNELAPNVPPEVVDLVHQMLQKVPADRPSILVVT
                     RELRRILGLNTEMSMPGQAVPMGGAPGMPSGPLRTTQRPPGLRTSQIGLRQTGAGAQG
                     PLQTGGNASLAGTSLRRSLAGLGVQPPAGGASDPASDPAGNSQNAMQNPAQAQSPKWQ
                     KFALIGVIGLVVAAGGALALKMSGGPEKPGGKTDDPKVQTPAKPEGLGAGKFWADVPS
                     NTTQVLQDLWGTGPSSLWAVGKNGTLLRWNGSAWANAELPASSNKSYLNAIWGSSAED
                     VWVVGKEGMILHWDGNKWNEVPSGAREGLKKVWGLSKDNVWAVGENGVILHWNGNDWS
                     PAPSGIDTELRGIWGSAANDIWAVGGSDASDGIILRWDGSKWATYGHVEARLNSLWGH
                     SAKDIWAVGGRPGPEGTVHHWNGTAWVQ"
BASE COUNT         1993 a         2961 c         2876 g         1759 t
ORIGIN      
        1 ctccattcgc ttcgtctcgc cgcccttctc actaatcagg atggagaaca tctcttggca
       61 cgctccttag agatggcttt gctgtcgagc aatagccgaa tcgattgcgc gataacttac
      121 cttttccgac gctgacaggt caagctcttt tgccaggcgg gcgccaccaa agtccgcatg
      181 cccagccgat tttttcgtcc gcaaaggtgc cgctaccacc ggacaaccgg gcggctttgg
      241 cgaccctgct tctgaaacaa tttccggacg accctggacg cgcaatatgt tgcgacgata
      301 tcaaattctg gccccagctt gaatagttcc gacttgttgt tgttgcttgg actgttaatg
      361 gccaaacatc ccaccttggt ggtgcatgtc gcagcctgtc gtctgatggc gccctgtctg
      421 cgcattatct tggcgtgcta tttcgtagtc ttatgagcat tttccgatcc gttctgtctt
      481 tcacgcgccc aaagtcccca atgtcgccaa agtctacgac cagcactgta tttgttcagt
      541 tggcaggggt cgcgcttctg ctggtgggca cgatcggcga tgcaaccgcc gcccccaagt
      601 acttcatcaa agtgcgcgac accgagcttg cggcggggat cgatgcaagc gtgccccaac
      661 atggcacccc agcggcaagc cctggcgcac aagacaagcc cgatgcggga actggcagcg
      721 cggccccggc gccccaagac ggcccaaaga ccgcggctga catggtgaaa aacgtgctca
      781 ccgaaacgct gcgcaaacag cctgaagtga cgctcgagct cgaaagtccg cttggcaatc
      841 ccgatgcagt gaaagccgag tgcaagcgca aaggtctgcg cgctcttgaa tttgccgttc
      901 gcgtgctcaa gatggcgcgc accaccaagc cgccggcggc tggcaagcga ttccggactt
      961 tggaacaagt ggtgaagctg tcggtggtcg gaaccacgat tcccgaaggt ctgctgtccg
     1021 ttggtggaga cggtgaatcg acgatccaga tcgaagtcgg cgagcgcatc acaccagggc
     1081 aagaacgcga agtgctggaa gagtcagtca aagacgccgt cgagcaagca gtcaagcagg
     1141 gcatgctgaa gctatcgcag cccgtcggca aaccgacccc ggaaaaacgg cggaaaaagt
     1201 aagcgccccc gccatcgttt tgtcgtgtgc gactgccggt gcggctagct cgaaagcagc
     1261 gccgccccgc gagcgatcag ctcctcgggt gccgaacttg gcaggggccg catcaccgca
     1321 tgcgatgcga ccagatcagc ccacagcggc agccgcgccc cgcctcccga tagcagcacc
     1381 gcatgaagct gggtggttcg cagccgcgcc gcccgcagcg caatctcgat cacatccagt
     1441 gcccgtcgca gctcatcttc gacaagaagt gcgagctgct cgcggcttag gtcggcaacc
     1501 aaatcgaccg ccgtatccgc aagtgtcagc aaacccggca gtcgcaccgt cacaagctcg
     1561 ctgtcgctca gttggcactt ggcccactcc gcagccaagc gcagccgcgc cagcgtcttg
     1621 cgatgcggcc acaccgacag accgttctgc cgctgaaacg cgcgccccag gtgatcggcc
     1681 actttttcat cgatcagttc gctgccgacc gcgcactgtg ccgtcgaaag caccgtaaag
     1741 cggcccgctt cggccccaag cagcgtgact tcgacatgca gcgcgccgat gtcgcagacc
     1801 atcactgctt ttccgtcggg cagctcgctg gcggacaggc cctgggcgcg ggcgatggca
     1861 agtggttcaa gcaccgtctg cgccacccga aaaccggcca gccgcgccgc atcaatcatc
     1921 gcttggtgct gttctggtgc gaaatggcat ggcacagcga tcgtcgcggc gtgcagtgga
     1981 ctgcccaacc gttcctcggc caagtctttg agctggctca gcacaacggc gcacagctcc
     2041 gtcgcagaaa accgcttgcg atccagttct agccgctgcg tcgtgccgac atagcgacgc
     2101 agattccccg ccgagcgttc cggccagagc aaaagctgcc gcctggcact gtcaccgaca
     2161 tgcagcgcgc cgcttttgtc aagcccaacc accgtcggaa caaaccgatg gccggtcagt
     2221 gtttcgagaa gcacactctc ggtcccctcg gtccacgctg cacgcgcagc gctgggacca
     2281 aagtcgatgc caatgcggga gaccgccatc gtaaggaagc tgagcgaacg ggactaaaga
     2341 atctcgacgc ccgttgcaac cagcgcgagg gtgtgctcgg gagcggtgac tttgcgcgcc
     2401 ggactgcctt ccttggccgc gtcgtcggcg tagtgctgcg cttccgccac cgggatggtc
     2461 ttgaccatca agtgaccttc gaccgtcgcg gtcttgccca tgcagtcctt cgggacaaag
     2521 aatccgtagt ccttaaacga aatccgcacc gacggagctt ccggttcgcc gctcgtcagc
     2581 gtcatccagc agcccttttt ctggcagacg cccgacacct tgccggtaag ttttacgtct
     2641 ttgtcgttcc atttctgcgg atcggcaatg acggtcttgg ctgcgattgc ctcggcgttc
     2701 gacagcggcg caccgtattt gcccggacct ggcgccggcg ctgactctgc cggtttggct
     2761 ggtgccgccg cactgatggt cggattcgca ggcgccggag ggctggcctg ggccggagcc
     2821 gtcggcgtcg ccggcttgtt gcagccaaaa agaaatgccg aaacggcaca gacaagaaca
     2881 gaaccgatcg tcggacgcat gaggcaaagc aagcccgctc gatcgcaatc tgtcaagtcc
     2941 cgcccactct tccaagttgg cgcagacgaa aaagatcggt gcaccggacc tgatccatct
     3001 tgaagcaggc aagtgcagct tcgccgaact tgcacagcct gttttttcgg cacactcgaa
     3061 gcgatttgtc gtcgctggtg tcatcaaaaa atggctacga ttccgcatgc tgtcggcaca
     3121 tggcacatcg cgtgcaaaag cccaagtcgt aaagacaacc cgcaacaagg attcgaccat
     3181 gtttagcgtg acgttttggg gagtacgcgg ttcgattgcg acgccaggag ccgacactgc
     3241 gaaggtcggc ggcaatacct cgtgcgtcga agtgcagtgt ggaacgcgcc ggatcgtgct
     3301 cgataccggg acggggatac gacagcttgg gcagaggctg ctgggacaga aaaaggccgc
     3361 ggcgcagccg gaaccgacgg accttacggt gttttacagt cacctgcact gggatcatct
     3421 gcaaggtctg ccgttttttg cgccgatcta ttcaccgacg accaagctgc acttttatgg
     3481 acccaaaggc gtcgagcgag cgctgcgcac ccagatgggc gatcccggct ttccggtgcg
     3541 catcgacgat gtgccggccc gcatcagctt tggcgagctg ggcgaaggca tggtcgtctc
     3601 gctggccgac gatctgatca tcagttgcgc gcggctgaat catccaggtg gggtgctcgc
     3661 gtatcgaatt cagtaccaag atcatgcgct ggtctacgcg acggacaccg aacactacag
     3721 cgtgcccgat cccaagcttg tgcgactggc gcgtgacgct gatgtgctca tatacgatgc
     3781 gcagtacgac gatgccgaat attccggcga aacgagtggc gtcccgcgca ccggctgggg
     3841 ccacagcacg ttccgcgaag gaatccgcgt cgccgacgcc gcgcgcgtcg gacagcttgt
     3901 gctatttcat cacgacccga gccacagcga cgatcaagtc gcgcacatcg aagcgacggc
     3961 acagcgcatc cgacccggca ccctggcggc ccgcgaagga cacaccattg cgattgctgc
     4021 tgccgaggag aaaccagcac tggcggtcgc cgcatgagcg gctttttgat accttacttg
     4081 gcgatgccgc gtgcgcctcg aaagaccgcg accagcggtg ctgaagattc atcgcgaagc
     4141 aacaccgtcg accggcgatc tgtgcttttg gcgcttttgg aactgatccg caccgaggtc
     4201 gatctggata cgctgctgcg acggatcgtc gatctgttgg cgcaagcgat ggacgccgat
     4261 cgggccacgc tgtttgtcgt cgacaagccc agtggcgagc tgatatcacg cgcggcccac
     4321 ttgccagagc tgccggaaat tcgcctgcgc ctaggtcaag gcattgcagg gctcgtcgcg
     4381 cagacccaaa aaccgatcag cttgtcgcac gccgatgatc acccgcgctt tgccgccaac
     4441 atcgatcgtc agacgggata caagaccgtg acggttttgg cagtgccgat tctctgcgat
     4501 tcgccgacgg gaaaccaaga acttctcggg gtgattgaag ttctgaacaa gcgcacaggc
     4561 gatttcaacg acgacgatcg cgcgctgctg tgcgagctgg ccgacgaagt tgccgcagcg
     4621 cttttgcaga cccggctctt gcagcgcgtc acccaaaaca cgcccgagcg ctaccaccga
     4681 atcatcggca catcgacgcc gatgctgcgg gtttacgaaa tcattgggcg cgccgcagcg
     4741 acgacagcga cggtgctgat tcgcggggaa tcggggaccg gcaaagagct cgcggcacgc
     4801 gcgattcact acaactcgca gcgggccaaa ggagcgtttg tcaaaatcga ctgcacgtcg
     4861 attcccgaag ggctgatgga atcggagctg tttggacacg agcgcggcgc gttcaccggg
     4921 gccgagcagc gggtgttagg caaggcggag ctggcagacg gaggaacgct gtttctcgat
     4981 gagattggcg atctgaaaag cagtctgcaa ggcaagctgc tgcggctttt gcaagatcgc
     5041 gagttcgagc gcgtgggcgg gcgaaaaacg ctcgcggtcg atgttcggat cgtcgctgcc
     5101 accaaccgac cactcgaagc gctggtcgaa tcggggcagt ttcgggccga tctgtattac
     5161 cggctgaaag tgatcgagct gtcgatgcct gcactgcgcg aacggagcgg cgacgacatc
     5221 gagcggctgg cgcagcactt cgtcaagcag tatgcgaccc gccacaacaa agcggtccgc
     5281 ggcatcagcg acgaagcatt gcgtcgactg cgcgcccacc tgtggcccgg caacattcgt
     5341 gaactggaac actgcatcga aagtgccgtg gcgctgtgtc ctgccgcgga gctatcgatg
     5401 gccgatctgc cactgccgac ggcttcccac gaagcatttt tgcggaaaaa tccacccgcc
     5461 cagccgccgc caacagacga agtgaaacca agtcgagtcg acccagctgg cttgcagctt
     5521 ccgtttggtc tgtcgctggc cgacgtcgaa aagcgctaca ttcagcagac cgtcgagcgg
     5581 ttcggtggca atcggtccga ggcggcgcgg gcacttggga ttgggcgaaa tacactgctg
     5641 cgcaaactgc gagagccgta gccgcgacag cgtttagggg aaaaccgcgc tcgccgtcga
     5701 aataaacgag cttggcgaca ccgagcactg ggcgtaacag cgcagcaccc cgcccgtcgg
     5761 acgggccgct tgcaagaagg tgtagattgt acccagtcca acaacgtggc gccgatcgcg
     5821 ctcgcgcttg atctcggcca ggaacgtctg cccgtccccc atcagaagcg gcttcagcgt
     5881 ctgtcgatca cgccgttcca gcgaatcgcg atcgtcatca ttgatttctt ggggatcgcc
     5941 aaagcgcggt ccgacgtggg ccaaatctgc cgacgcgatc cacagcaccc gctcgccagc
     6001 gccctgcttt tcgccgaccc gctgctggat gagatgcacc acttcatcca taagccgcgt
     6061 gtcctgcgct tcgtgcgcgc gactgcccga cgctgccgcg aagtcatgaa gcgatccgca
     6121 cagcaccggc acgaccttgg gatcggcatc tttgtgaccg cgctttttcc gctgatccaa
     6181 gacaaaccgc agccagatcg ccaaaagctc cagcgagtgc tcgttgcgat gatgaaactc
     6241 gtcgcgcacc accagctcgc caagctccgt acttttggcg gtcgcgtcgg cgatgatcgc
     6301 gtcgaccaac tcgacgtcgg tttgcagttc accgatcggt gtctggtagt gtttgcgcgt
     6361 cagcgtcagc atcgggtccg cagccccatg atcgcagccg agcaagacga tcagcgtcgg
     6421 aaattccgcc gcatcaagca gcggtttgag cgcccacgca taggccggtc cgcccgattt
     6481 gatttccacc gacggcagca gcatcgcccg tggcgctttg ccggcagttt ctggcacagc
     6541 caggcccggc ccgttcgggg ctgcaaacgc cgactcaagt cctttgatca gatcgtcaac
     6601 gcccgtcgga tagagcgtcc cggcaccgag cggtggacgg ctgggacttt gggcaaagtc
     6661 ggcataaatt ttggcgcagt gcagacggaa attggtcgaa tcgagcagca gcgcatcatc
     6721 caactttttg agcagcgctt cgagcgcatc ggcggtcaaa gcccgccggt aacgcgcggc
     6781 aaactccgcc aaaatttccg cccggttgcg cgtgccatca catagatcga tgactgcggc
     6841 accaagcggc ggcaacgtca ccggctgcgg cgcaatcccc gacggatcat agagcgccag
     6901 aagcttttga ccgcctgggc catcgacggg aaatgcttcc agcggtcgca gcatcggctt
     6961 ttcttcgctg ggatcgatcg cagcgtcggc gatgtcttct ggatcgtcgc tggcagagac
     7021 agactctgtc accgtagcag aagcggtttg cgtcacggac tcggcagcac ccgatcccgg
     7081 cgccgcctgc gtcccaccct ctttatcgtt agaagataca gacatggagt gagtataacc
     7141 gccttagata cgtaaccgtt cctggattga tgatgagtgt ttggatgtca gcgtaggtgc
     7201 gcagatgtat ttccagttac gacatcggcg acattacaat tacaaatttg gcaaatttga
     7261 cctgtgattt tttcacaaac agcgctgtac caaattgcac ataaaacgga tcgggaacca
     7321 ggactctcat cccgcaatca attcgcgtag atagcaactt ttccgacgaa aaaccaggat
     7381 cagacggcgc tgcttcgcgg tgggtgcagc agtgtggatg taaccacagt gaagacaaaa
     7441 acccagtttg agtaaagcag tgggaaagca tggcgttcct gaatatctgc cgggattaca
     7501 actagttttg attgatgtag aatcgcagat cgtagaacat gtttactcag ggctgcttag
     7561 caactaactc accgattgtg gtgggttaag actacggcct tgccccaaga gaacagccac
     7621 aaggtcattt catggccgac cagcgaatcg gaaattaccg gattgtgcgc aagcttggcg
     7681 aaggtggaat gggcgtcgtc ttcgaagcca tccacgaact tatcaatcgt cgagccgcaa
     7741 tcaaggtgct acacgccgac ttctctcgga atcccgagat ggcaacgcgc ttcctaaacg
     7801 aagcccgcgc ggccaacatc gtgcagcacc caggcatcgt caacatcttc gagttcgatc
     7861 gccttcccga cggtagcgca tacatcgtca tggattacct cgacggggat tcgctcacgc
     7921 agcgactcca gcgtctgggg ggtcggatgg aaacaggacc ggcgctgtcg ctgatcctgc
     7981 aagtcgccga agcgcttacg gctgcacact cgaagggcgt catccatcgg gatctgaagc
     8041 cggacaacat catgattgtt ccggaccagg gcgactccag ccgcgaacag gccaagatcc
     8101 tcgactttgg catcgccaag atggccgagt cgcagggcat gggtccagcg ctggcgaaaa
     8161 cccaggtcgg tgttccgatg ggaacgccgc tttatatggc accggagcag tggaaaggcg
     8221 cgagcgaagt cgaggacaaa gccgacgtct actcgctcgg cgtcatcatc tacgagatgc
     8281 tgtcggggca gccaccgttt atcgcaaact ccggcagcga ggtcatggcg ctccacattc
     8341 tggcgacgcc gcggcctctc aatgagctgg cgccgaacgt cccgccggaa gtcgtcgatc
     8401 tggtgcatca gatgctccag aaagtgccgg ctgaccgacc gtcgattttg gtggtcacgc
     8461 gcgagctgcg ccgcatcctc ggcctcaaca ccgaaatgag catgccgggt caagcggtgc
     8521 cgatgggcgg tgcgcccggc atgccgagcg gaccactgcg cacgacgcag cgcccgccgg
     8581 gacttcgcac gtcgcagatt ggtcttcgtc agaccggcgc aggcgcgcaa ggaccgcttc
     8641 agacgggcgg caacgcgagt ctggccggaa cttcgctcag gcgttctctg gctgggctcg
     8701 gcgtccaacc gccagcggga ggagcgtctg atccagcttc agacccagca ggtaactctc
     8761 aaaacgccat gcagaaccca gcgcaagcac aaagtcccaa gtggcagaag ttcgcgctca
     8821 tcggggtcat cggccttgtg gtcgccgccg gtggtgcgct cgctctcaag atgtcgggcg
     8881 gcccagaaaa gccgggcgga aaaaccgacg atcccaaagt tcagaccccg gccaaacccg
     8941 aaggtctggg cgccggcaag ttctgggcag acgtgccaag caacaccacg caagtgctcc
     9001 aggatctttg gggcactggc ccgtcgagcc tgtgggccgt cggcaaaaac ggcacgctct
     9061 tgcgctggaa cggctcggct tgggccaacg cagagctacc cgcaagctcg aacaagtcct
     9121 atctgaacgc gatctggggc agctcggcag aggatgtctg ggtcgtcggc aaagaaggca
     9181 tgatcctgca ctgggacggc aacaagtgga acgaagtccc gtcgggtgcc cgagaagggc
     9241 tcaagaaggt ctggggtctt agcaaagaca acgtgtgggc cgtcggtgaa aacggcgtca
     9301 tcctgcactg gaacggcaac gactggagcc cggcgccaag cggcatcgac accgagctgc
     9361 gtggcatctg gggcagcgcc gccaatgaca tctgggccgt cggtggttca gatgcatcgg
     9421 acggtatcat cctgcgctgg gacggcagca aatgggccac ctacggccac gtcgaagcgc
     9481 gtctcaactc gctgtgggga cattcggcca aggacatctg ggccgtcggt ggtcgtcccg
     9541 gtcccgaagg caccgtacat cactggaacg gcaccgcttg ggtccaaga
//
LOCUS       DAORGN010000188         9576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3105_length_9576_cov_7.197143, whole genome shotgun sequence.
ACCESSION   DAORGN010000188 DAORGN010000000
VERSION     DAORGN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9576
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3105_length_9576_cov_7.197143"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKL17_17160"
     CDS             <1..709
                     /locus_tag="PKL17_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNK46515.1"
                     /translation="HLHVRDEAGNPSQDRERFGAAIAAIRSRCDVVVQTSTGGAVGMS
                     IDERCGPLLLSGRERAEMATLNVGTINFGDDIFVNRRLDTFGVAERIKAAGIIPEIEI
                     YDLGHLDILEELVAKGAVPRPLHVQFVLGVRGALKASERNLELLAARLDEDFPGSTWG
                     VAGVGRYELPMAEVAARIGGNIRVGLEDNIFIEKGVLAKGSVELVEKAVAICQAAGRK
                     VASIAEARAVLLQKPQP"
     gene            706..1311
                     /locus_tag="PKL17_17165"
     CDS             706..1311
                     /locus_tag="PKL17_17165"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNK46516.1"
                     /translation="MKPPLCGIFVGGAAKRMGGYPKGLLPAGPSDQPIVLRLAELAKS
                     LGLPVVFVGQHAAYQDLGLPLLADRPPGVGPLGGLAALLHAADGADVVALACDMPFVT
                     TDFVKKLIDADLADADAIVAQTAPTAKLESFLARYRPSVLPVIEQKLAEGERSLQRIL
                     HGLRTRTLLIPPDEQTVCRDWDKMDDLPSELVAKLTRRDVT"
     gene            complement(1304..2392)
                     /locus_tag="PKL17_17170"
     CDS             complement(1304..2392)
                     /locus_tag="PKL17_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNK46517.1"
                     /translation="MTQATVDGVRSALKAVKEKALGRDIVSLGMVKKIEVEKDFAYVQ
                     VDLPTPVYRDRSELEKNVQKAACTAPGIKDARCLLGVNVPSGSRGQATRLPSVKNVIA
                     VAAGKGGVGKSTVATNLAASLRLQGARVGLLDADVYGPSLPTMLGEPEVAASTRAGNK
                     IGPAVYYGMSVISVGFFTERDGAVVWRGPMVHKLLQQFIEDVDWGDLDYLIVDLPPGT
                     GDAQLSLTQLLPLTGVVIVSTPQEVALIDVEKAVSMFKKLEVPILGVIENMSYYICPT
                     CGHHDDIFARGGGAKLAENLKVPLLGEVPLDRKVRYGGDSGCPIVISATDSEHGKIFM
                     EVGQRVAEGAMKVVLSGPRRPAGLVQVK"
     gene            complement(2409..3068)
                     /gene="ribA"
                     /locus_tag="PKL17_17175"
     CDS             complement(2409..3068)
                     /gene="ribA"
                     /locus_tag="PKL17_17175"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNK46518.1"
                     /translation="MSESTPSTAAAKVGSALYRYAESEIPTVHGNLRLLVYRHVQKDQ
                     GPAPLIVPLQDEHMAIVVGDVSGGEQVPCRVHSECWTGETLGSLKCDCRAQLDAALQS
                     MVSVGRGVVVYLRQEGRGIGLGNKIRAYALQEGGKDTVEANTELGFSPDLRSYDIAAQ
                     ILKDLGVSSVALYTNNPDKLRELAEAGVKVSERVPHWVSIHEWNSDYLAVKKAKLGHQ
                     S"
     gene            complement(3098..5596)
                     /gene="gyrB"
                     /locus_tag="PKL17_17180"
     CDS             complement(3098..5596)
                     /gene="gyrB"
                     /locus_tag="PKL17_17180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNK46519.1"
                     /translation="MSEKAGNPAESDSGAAAPAKESYTGENIQVLKGLDAVRKRPGMY
                     IGDTDDGTGLHHMVYEVVDNSVDEALAGHCKNIEVTIHFDNSVTVTDDGRGMPTDMHP
                     TENRPTPEVIMTVLHAGGKFDKKSYKVSGGLHGVGVSAVNALSEWLKLEIRRAGKVYH
                     QEYRRGAPVTPLTEMGVSTKNGTTVTFKADADIFAKVEYSYEVLHQRLRELSFLNSGL
                     RIVLTDERNDKKADMFSDGGISSFVKDLNVAKELVVADPIFVTGSQPHPDIEGTEVIA
                     EAAIQWNDSFSENVFCFTNNIRNRDGGSHLTGFRQALTRTINAYATANNLLKDMKQGL
                     SGADLAEGLVAVVSIKHPDPKFSNQTKDRLVSSEVTGIVNSVIGDRLAEWLEEHPREA
                     RAIIGKSVLAARAREAARKAREMVQRKGALESLSLPGKLADCQERDPALCELFIVEGD
                     SAGGSAKQGRDRRTQAILPLKGKILNVEKARLDKMLSSQEIVTLITALGCGVGDSKDM
                     AKLRYHRIVIMTDADVDGSHIRTLLLTFFYRQYKELLDGSLDRKHFIYIAQPPLYKVK
                     KGSREEYLKNDQALEEYLLRSTAEDLVFESQDGKLQVTGDKLRELWRLVGRYARALSA
                     CDKKWDSRIVEALLHVGELQVSSLREEEAVRAALGRAEEKLKQHFSDVTDLQAQLSPD
                     AEHGTIRAVIPARYGGARRETVVDFALFTSPEYAELGRIYDRLRQFGHGPFVLVDGSN
                     RHNVARLSELASVLDTIGRKGLQIQRYKGLGEMTAEQLWETTMDPARRTLLEVHVDDL
                     NEAERLFSMLMGEAVEPRREFIEQHALQVRNLDV"
     gene            complement(5724..6923)
                     /locus_tag="PKL17_17185"
     CDS             complement(5724..6923)
                     /locus_tag="PKL17_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNK46520.1"
                     /translation="MLLRSLHTLDFRNLRDVEYRPHPRFNVIFGDNGQGKTNLLEAIF
                     LVATQRAFRSSKLDEICRFGTGKAEVIAAVSHADVERRLAIQLFTKPSRKVALVDGKS
                     ARSSDYFTGVHVVLFAPEDLLLPKGPPEDRRRFLDRAIWNTCPNYLDEVRVYEQVLKS
                     RNAVLRSMLLQTGRPHRSDSFGDAGMQISSGGDSDAMLAVYTEQLAQAGARIIERRRH
                     YVRTIAPQVEATFERISRSGLAANVSYRPKLSAHLQDRAVDRAETPELCEILHEQFER
                     DTRRDRLRGFTHSGPHGDDMLVSLDGREAALHASQGQVRALVLALKITEIQHLFFLHG
                     DPPVLLLDDVSSELDAQRNGYLFDFLRAMPSQVFITTTSPEYVKLSQDRLDVRMQMGA
                     LQTHLQS"
     gene            complement(6928..8058)
                     /gene="dnaN"
                     /locus_tag="PKL17_17190"
     CDS             complement(6928..8058)
                     /gene="dnaN"
                     /locus_tag="PKL17_17190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNK46521.1"
                     /translation="MEFRIEKAAFLQALYYAQGIADRKSTMPILANVLLRSDGPERIL
                     VAATDLNLTVTTELPCEVQSEGGLTASAKHLHDIVKSLPSDKLGLRRADNNYAELSSG
                     KANYKVVGLPDRDFPKLPPHRDVKFHKVDGAVLRDLIAKTFFSISADETRSHLNGVLL
                     ECEGEGEQAFARMVSTDGHRLSKCERPLPGAPKLMPGVVIPRRGLMEIRRALEGSGGS
                     VEIAAQNQYFFVRTGATVLSVRLTEATFPPYEQVIPKDHQKRIVVSSGKLLDALKRIV
                     LVASEKTWGVRMSIVGGSMRIESDNPDLGQGREELDVNYQGSDCTIGFNAKYMIELLS
                     EIDTPEVQLELNGDLDPGVLRPLDEKQGVGNQYLGVIMPMRL"
     gene            8421..9164
                     /locus_tag="PKL17_17195"
     CDS             8421..9164
                     /locus_tag="PKL17_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46522.1"
                     /translation="MSLSKMNKRRSNRAWYLATSLGVLLTEKVVPMGVALSLLATSGC
                     LKLLTEKPLEPGSYGSTEPELLRLWQDVLEAAKRDDRDKLTELMSSMRLSQDELATLV
                     GDDKARRFWPRYELLARPLGAPGAAELVGSIYEHHYDDVEVTRVDTLPVEKLSESEQG
                     VRRALLKPTPFYRVRVIKKGTPYAIRYDFFVYQHGFWRTGRELGKFLEPQKIVPLAPA
                     PPPDAGVPKPDAATVDMSPQKVDTKKSPG"
     gene            9217..9576
                     /gene="ssrA"
                     /locus_tag="PKL17_17200"
     tmRNA           9217..9576
                     /gene="ssrA"
                     /locus_tag="PKL17_17200"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1800 a         2878 c         2868 g         2030 t
ORIGIN      
        1 tcacctgcac gtccgcgacg aggccggaaa cccgagccaa gaccgcgagc ggttcggggc
       61 agcgattgcg gcgattcgca gccgctgcga tgtcgttgtg cagacatcga cgggcggtgc
      121 ggtggggatg tcgattgacg agcgctgtgg tccgcttttg ctttcggggc gcgaacgagc
      181 cgagatggcc acgctgaacg tcggcaccat caactttggc gacgacatct ttgtgaatcg
      241 gcggctcgat acgtttggtg tcgccgagcg catcaaggcc gccggcatca ttcccgagat
      301 tgaaatctac gacctcggac acctcgacat cctcgaagag ctggtcgcaa aaggcgcggt
      361 gccaaggccg ctgcatgtgc agtttgtcct tggggtgcgc ggggcgctca aggccagtga
      421 gcggaacttg gagcttctgg cagcgcggct tgacgaggac tttcccggct cgacgtgggg
      481 tgtggcgggc gtcggacggt acgagctgcc gatggcggaa gtggcggcgc gcatcggtgg
      541 caacatccgc gtcgggctgg aagacaacat cttcattgaa aagggcgtgc tggccaaagg
      601 ctccgtcgag ctggtcgaaa aagcggtcgc gatctgccag gcggcgggac gaaaggtcgc
      661 ctcgattgcc gaagcgcgcg ccgtgctgct gcaaaaaccg cagccatgaa acccccgctt
      721 tgcggaatct ttgtcggtgg cgctgccaaa cgaatgggcg gctatccgaa aggccttttg
      781 ccggctggcc cgtctgatca gccgattgtt ctgcggcttg ccgagcttgc aaagtcgctg
      841 gggctgccgg tcgttttcgt cggacaacat gccgcgtatc aggacttggg actgccgctg
      901 cttgcagaca gaccgcccgg cgtcggtccg ctcggtggac ttgcggcgct gcttcacgcg
      961 gcagatggtg cggatgtcgt cgcgctcgct tgtgacatgc cgtttgtcac aacggacttc
     1021 gtcaagaagc tgatcgatgc agacctggcc gatgcagacg cgatcgtcgc ccagactgcg
     1081 ccgaccgcga agctagagtc ttttttggcg cgctatcgtc cgtcggtgtt gccagtcatc
     1141 gaacaaaagc ttgccgaagg cgagcggtct cttcagcgaa tcttgcacgg tctgcgcaca
     1201 aggacactgc tgattccgcc cgacgagcag acggtgtgtc gcgactggga caaaatggat
     1261 gatctgccca gcgagctggt ggcgaagctg acgagacggg acgttacttg acttgcacca
     1321 gaccggcggg tcgacgcggg ccggacagca caaccttcat cgcaccttcg gcgacgcgct
     1381 gaccgacttc catgaagatt ttgccgtgct cgctatcggt tgccgaaatc acaattgggc
     1441 agccgctgtc gccgccgtag cgaaccttgc gatcgagcgg tacttccccg agcagcggca
     1501 ccttgaggtt ttccgcaagc ttggcaccgc cgccccgcgc aaagatgtcg tcgtggtggc
     1561 cgcaggtcgg gcagatgtaa taggacatgt tttcgatgac gccgaggatc ggcacctcga
     1621 gctttttgaa catgctgacg gctttttcga cgtcgatgag cgcaacttcc tgcggcgtcg
     1681 agacaatcac cacacccgtc agcggcaaaa gctgggtcag cgaaagctgc gcgtcgccag
     1741 tccccggcgg caaatcgacg atcagatagt ccagatcgcc ccagtcgacg tcttcgataa
     1801 actgctgaag cagcttgtgc accatcgggc cgcgccacac cacggcgcca tcacgctcgg
     1861 tgaaaaagcc gacggatatg acgctcatcc cgtaatagac cgcggggccg attttgttgc
     1921 cagcgcgcgt cgaagccgcg acttccggtt caccgagcat ggttggcagc gacggaccat
     1981 agacatcggc atcgagcagc ccgacccgcg ccccttgcag tcgcaaagaa gccgccaggt
     2041 tggtcgccac cgttgatttg ccgacgccgc ctttgcccgc cgcaactgcg atgacgttct
     2101 ttaccgacgg aagccgcgta gcttgcccac gacttcccga tggaacattg acgcccagaa
     2161 ggcagcgcgc gtctttgatg cccggcgctg tgcaagccgc cttctgcaca tttttttcaa
     2221 gctcgctgcg gtcgcgatag accggcgtcg gcaggtcgac ctgcacatac gcgaagtctt
     2281 tttcgacctc tatttttttg accatcccga gcgacacgat gtcgcgaccg agcgccttct
     2341 ccttcaccgc tttgagcgcg ctgcgaactc catcaaccgt ggcttgcgtc attgccttct
     2401 tctcttgtct agctttgatg tccgagcttg gcttttttga ccgccagata atcgctattc
     2461 cactcgtgga tcgataccca gtgcggcact cgctcgctga ctttgacacc cgcctcggca
     2521 agctcacgca gcttgtcggg gttgttggtg taaagcgcca ccgacgacac gccgaggtct
     2581 ttgaggatct gcgcggcaat gtcgtagctg cgcaaatcgg gggaaaagcc aagttccgtg
     2641 ttcgcctcga cggtgtcttt gccgccttct tgcagtgcgt aggcgcgaat cttgttgcca
     2701 agaccgatgc ccctgccctc ttgccgcaga tacacgacga caccgcgtcc aacgctgacc
     2761 atgctttgca gcgcggcatc gagctgggct cggcagtcac acttgagcga tccgagtgtt
     2821 tcgccggtcc agcactcgga atggacgcga cagggcactt gctcgccacc gcttacgtca
     2881 ccgacgacga tggccatgtg ctcgtcttgc aaaggcacaa tcagcggcgc agggccttgg
     2941 tctttttgga cgtgccgata gaccaaaagc cgcaggttcc cgtggacggt gggaatctcc
     3001 gactctgcat agcgatagag cgcgctgccc actttggcag cggcagtcga aggcgtgctc
     3061 tcggacatgg gaaatgtaaa gatgctgctc ggcgcactta gacgtcgagg ttgcggacct
     3121 ggagggcgtg ctgttcgatg aattcgcggc gcggctcgac ggcttcgccc atcagcatgc
     3181 taaacagccg ctcggcttcg ttgagatcgt cgacgtgaac ttcgagcagc gttcgccgtg
     3241 ccgggtccat cgtcgtttcc cacagctgct cggcggtcat ttcgccaaga cctttgtagc
     3301 gctgaatctg aagacctttg cgcccaattg tgtcgaggac agacgcaagc tcagatagcc
     3361 gtgcgacgtt gtgcctgttc gatccatcga cgagcacaaa cgggccgtgg ccaaactgtc
     3421 gaagtctgtc gtaaatgcgg ccaagctcgg cgtattcggg cgacgtaaac agcgcaaaat
     3481 cgaccaccgt ttcgcgtcgc gccccgccat aacgcgccgg aatcaccgcc cgaatcgttc
     3541 catgttcggc atcgggcgaa agctgcgctt gcaggtcggt cacgtcggaa aagtgctgct
     3601 tcagtttctc ttccgcccgt ccgagcgccg cccgcaccgc ttcttcttcg cgcagcgagc
     3661 tgacctgcag ctcgccgacg tggagcagcg cttcgacgat gcggctgtcc cactttttgt
     3721 cgcaggccga aagcgcgcgc gcatagcgac cgaccagccg ccacagctca cgcagcttgt
     3781 cgccggtgac ttgcagcttg ccgtcttgcg attcgaacac caagtcttct gccgtcgagc
     3841 gcagcaggta ctcttcgagc gcttgatcgt ttttcagata ctcttcgcgg ctgcctttct
     3901 tcactttgta aagcggcggc tgcgcgatgt agatgaaatg tttgcggtcg agcgacccgt
     3961 cgagaagctc tttgtactgg cgatagaaga acgtcaaaag cagcgtgcga atgtggctac
     4021 cgtcgacgtc ggcgtcggtc atgatgacga tgcggtgata acgcagcttc gccatgtctt
     4081 tgctgtcgcc gacgccgcag ccaagtgccg taatcagcgt cacgatttcc tgcgacgaca
     4141 gcatcttgtc gagccgcgct ttttcgacgt tcaggatctt gcctttgagc ggcaaaatcg
     4201 cttgggtccg acggtcacga ccttgtttgg ccgatccacc tgccgaatca ccctcgacga
     4261 tgaacagctc acacagcgcc ggatcacgtt cttgacagtc ggccagcttg cccggcagcg
     4321 acagcgactc aagcgcacct ttgcgctgca ccatttcacg ggctttgcgc gctgcttcgc
     4381 gggccctggc tgccagcacg ctcttgccga tgatcgcgcg cgcttcccgc gggtgttctt
     4441 cgagccactc ggccaggcga tcgccaatca ccgagtttac gattcccgtc acttccgacg
     4501 acaccagccg gtctttggtc tggttcgaaa acttcggatc ggggtgtttg atggacacca
     4561 ccgcgacgag accttccgcc aaatcggcgc ccgaaagacc ctgcttcatg tcttttagca
     4621 ggttgttcgc cgtcgcatag gcattgatcg tgcgggtcag cgcttgccta aagccggtca
     4681 ggtgcgatcc accgtcgcgg ttgcggatgt tgttggtaaa gcaaaagacg ttttccgaaa
     4741 agctgtcgtt ccactggatc gcagcctcgg cgatgacttc agttccctcg atatcgggat
     4801 gcggctggct accggtcacg aagatcggat cggcgacgac cagctctttg gcgacgttta
     4861 ggtctttgac aaagctcgaa atgccgccat cggaaaacat gtcggctttt ttgtcgttcc
     4921 gctcgtcggt cagcacaatc cgcagaccgg aatttaggaa cgacagctcg cgcagccgct
     4981 ggtgcagcac ttcatacgag tattcgactt tggcaaagat gtcggcatcg gctttgaacg
     5041 tcaccgtcgt gccgttcttc gtcgatacgc ccatctcggt cagcggagtc accggcgcgc
     5101 cacgacggta ttcctggtga tacaccttgc cagcgcgacg gatttccagc ttgagccact
     5161 cggacagcgc gttgaccgcc gacacaccga cgccgtgcag accgcccgat actttgtagc
     5221 tctttttgtc gaactttccg ccggcgtgca gcaccgtcat gatgacttct ggcgtcgggc
     5281 gattttccgt cggatgcata tccgtcggca tgccacgtcc gtcgtccgtc accgtcaccg
     5341 agttgtcgaa gtgaatcgtg acttcgatgt tcttgcagtg acccgcgagc gcttcgtcga
     5401 ctgagttgtc gacgacttcg tagaccatgt gatgcagtcc ggtgccatcg tcggtgtcgc
     5461 cgatgtacat accgggccgt ttgcgcaccg catccagacc cttgagaacc tggatgtttt
     5521 cgccggtgta gctctctttc gcgggagcag cagcgccgct gtcactctcc gctgggttgc
     5581 cggccttttc actcatagcc aaagaaagct cctcgaattg cctggcagcc ggtctctacg
     5641 gggccgccaa aagaggtcgc aaggtagcac agctttcccc acttttcgcc tttttcccgg
     5701 tgcttttcgc cgctgcgccg gagctagctc tgcaaatggg tctgcagcgc gcccatttgc
     5761 atccgcacat ccagccggtc ttgcgacagc ttcacgtatt ccggactcgt cgtggtgatg
     5821 aagacttggc tcggcatcgc gcgcaaaaaa tcgaacaggt agccgttgcg ctgcgcatcc
     5881 agctcactcg acacatcgtc gagcagcagg accggcgggt cgccgtgcag aaaaaacagg
     5941 tgctgaattt cggtgatttt cagcgccagc acgagcgctc gcacttggcc ttggctcgcg
     6001 tgaagtgccg cctcacgtcc gtcgagactg accagcatgt cgtcgccatg cggtccgctg
     6061 tgggtaaagc cgcgtagccg gtctcgtcgg gtatcacgct caaactgctc gtgcaggatc
     6121 tcgcacagct ccggcgtttc ggctctgtcg acggcgcgat cctgcaaatg ggcggacagc
     6181 ttgggccgat agcttacgtt ggcggccaga ccgctgcggc taatccgctc gaatgtcgct
     6241 tcgacctgcg gcgcaatcgt gcgcacatag tgccgtcgcc gctcgatgat gcgagccccc
     6301 gcttgggcga gctgctcggt gtataccgcg agcatggcat cactgtcgcc cccgctggaa
     6361 atctgcatac ccgcgtcgcc aaatgaatca ctgcgatgtg gccttcccgt ctgaagaagc
     6421 atgctgcgca agacggcgtt gcggcttttt agcacctgtt cgtaaactcg tacctcgtcg
     6481 agatagtttg gacaggtgtt ccagattgcg cgatcgagga agcgtcgacg gtcttccgga
     6541 gggcctttgg gcaacagcag gtcttctggc gcaaacaaca cgacgtgaac gccagtgaaa
     6601 taatcacttg aacgcgcgga ctttccgtcg acaagagcga cttttcggct gggtttggta
     6661 aacagctgaa tcgccagtcg ccgctcgacg tcggcgtgag ataccgcggc aatcacttcg
     6721 gctttgccgg tgccaaatcg acaaatctcg tcgagcttgc tgctgcgaaa cgctctttgg
     6781 gtcgcgacca gaaatatggc ttcgagcaag ttggtcttgc cctggccgtt gtcgccgaaa
     6841 atgacattga agcgagggtg gggccgatac tcgacgtcgc gtaggttgcg aaagtcgagc
     6901 gtatgtaagc tgcgcagaag caaccgctca caggcgcatg ggcatgatca cgccgagata
     6961 ctggttgcca acgccctgct tttcgtcgag cgggcgcagc acaccgggat ccaggtcgcc
     7021 gtttagctcc agctgcactt ccggcgtgtc gatttccgac agaagctcaa tcatgtactt
     7081 ggcgttgaag ccgatggtgc agtcgctgcc ttggtaattt acgtcgagct cttcgcggcc
     7141 ttggcccagg tcggggttgt ccgactcgat gcgcatgctg ccaccgacga tgctcatgcg
     7201 gacgccccag gtcttttccg acgcgaccag cacaattcgc ttgagcgcgt cgaggagctt
     7261 cccgctgctg acgacgatgc gcttttggtg atctttggga atcacctgct catacggagg
     7321 gaaggtcgct tccgtcagcc gcaccgacag cacggtcgcg ccggtgcgca cgaaaaagta
     7381 ctggttttgc gcggcaattt cgacgcttcc gccgctgcct tcgagcgccc gccgaatttc
     7441 catcagaccg cgacgcggaa tcaccacgcc aggcatcagc ttcggtgccc ccggcagcgg
     7501 acgttcgcac ttcgacaggc gatgaccgtc cgtcgatacc atccgagcaa acgcctgctc
     7561 gccctcgcct tcgcactcga gcagcacgcc gtttaggtgg ctgcgcgtct cgtcggcaga
     7621 aatcgaaaag aacgtctttg cgatgagatc gcgcaggacc gctccgtcga ctttgtgaaa
     7681 cttcacgtcg cggtgaggcg gcagcttggg gaagtctctg tcgggcagac caacgacttt
     7741 gtagttcgct ttgccgctcg aaagctcggc gtagttgttg tcggcgcggc gcaggcccag
     7801 cttgtcgctt ggcagcgact tgacgatgtc gtgcaggtgc ttggctgacg cggtcaggcc
     7861 accttcgctc tgcacttcac acggcagctc ggtggtaacg gtcaggttca ggtcggtcgc
     7921 ggccaccagg atgcgctctg ggccatccga gcgcaaaagg acgttggcta agatgggcat
     7981 ggtcgacttg cggtctgcaa tgccctgcgc gtagtacagc gcttggagga acgctgcctt
     8041 ctcgattctg aactccatct gtttctccaa cctgggtttg ttgccttggg ggtgcggttc
     8101 tacctgatct ggatcaatag aaatagatcc aattcgtatc cgtattcggg cctgtggata
     8161 tctggaaaag tcaccaaagt ccttggaaat caagtacttc gcttgtggat gaagatgtgg
     8221 gacactgtgg aaaaagcacc cagtcttcca cagccggtgg ttgttcccac cctatccact
     8281 gaccaattca gagggttacc cacagagtta tccacagccc cacgatccag gcgatgtcat
     8341 accgcaggtc gggcaggatc ggcaagggtg cgtgggtttt tgcagacgga gccaagctgt
     8401 gcgacaagca aaagcacatc atgtctttgt cgaagatgaa caagcgaagg tcgaatcggg
     8461 cttggtattt ggcgacgagc cttggggttt tgctgaccga aaaagtggtg cctatggggg
     8521 tcgccttgtc gcttcttgcg acgagcggct gtctcaagct gcttaccgaa aaaccgctcg
     8581 agccgggcag ctacggcagc accgagcccg agcttttgcg gctgtggcaa gacgttttgg
     8641 aggcggccaa gcgcgacgat cgcgacaagc tgaccgagct tatgtcgtcg atgcggctgt
     8701 cgcaagatga gctagcgacg ctggttggcg acgacaaagc gcggcggttt tggcctcgct
     8761 atgagctgtt ggcgcggccc cttggggcac cgggcgcggc agagctggtc ggcagcattt
     8821 acgaacacca ctacgatgat gtcgaagtga cgcgcgtcga cacgctgcct gtggaaaagc
     8881 tgagcgagag tgagcaaggg gtgcggcgcg cgctgctcaa gccgacgccg ttttatcggg
     8941 tgcgagtcat caaaaagggc acgccctacg cgattcgcta cgattttttc gtctatcagc
     9001 acggcttttg gcgcaccggg cgtgagctgg gcaagttcct ggagccgcag aaaatcgtgc
     9061 cgctagcgcc cgcgcccccg cccgacgccg gtgtcccaaa gcccgacgcc gcgacggtgg
     9121 acatgtcgcc acaaaaggtc gacaccaaaa agtctcccgg ttgacgagtc ggcatcgcgt
     9181 ggctaagctg cctttgccct tggttgctcc catttcgggg gcgtaacggt ttcgacgggg
     9241 gtgatctgag tagacggttg cgtgtcgagg attcgttacc tcgtaaatcc agcggaaaaa
     9301 tccgaaacgc gaacgataac gcgtacgctc tcgctgctta ataaccagac agagagccgc
     9361 ctccctcggg tagttctcgc gacttgagac ggggtgtcaa aataaacgag atagctccct
     9421 cgatgtccat cgcgaggcca gcgaaaccaa aggtggaata gcccaagggg tcatttgtcg
     9481 gcggatgtcc tcagcgggtg aaaaaaacac cgactacaca cgtagtagcc cctgccaagc
     9541 actttcggac ccgggttcga ctcccggcgc ctccaa
//
LOCUS       DAORGN010000189         9551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3122_length_9551_cov_5.590775, whole genome shotgun sequence.
ACCESSION   DAORGN010000189 DAORGN010000000
VERSION     DAORGN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_9551_cov_5.590775"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKL17_17205"
     CDS             <1..213
                     /locus_tag="PKL17_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNK46523.1"
                     /translation="QQQGLVWVADAELSKFLRQRHPNIRRVYSQGQPRSETRAAGKEA
                     GERLILHKPIHGPATTGRATKLLPGR"
     gene            324..1457
                     /locus_tag="PKL17_17210"
     CDS             324..1457
                     /locus_tag="PKL17_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46524.1"
                     /translation="MSKDPLAPDNLANLRRTLPGLGVSDGSPLQVQEVSAADVERVLA
                     ATQTPPNPNPSPASSTGVGLGGRSVSQTGIPVLAGTSNPAIPQLRGTSNPAIPQLRGT
                     SNPAIPMLRSTSNSAMPLLRTTGNPEPPILPRASETSIPSVPPSSNRLPLLIAGLGTL
                     ALAIGVVAWLHPGQSATGSGVPADKTAVLSLPTALPTGGSQAVVPVRTVAQPAPVVVV
                     APAKDKPSDDKKDQISARKPPEETGHVVTSSKPTTSPPATGNPAKPVAPKAVATKVEA
                     KPTRAPQLDLSPVPQLTRDILRQHMTVAEPKWQACPKDGSGKYLTIGIAVAPSGSVVK
                     AEVMGPAGSTQTAQCITERIRGMRFPAYSEGGPKNFVWSYQVP"
     gene            1642..3621
                     /locus_tag="PKL17_17215"
     CDS             1642..3621
                     /locus_tag="PKL17_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46525.1"
                     /translation="MFCASCKGIFPIEATVCDRDGSPLERAEQVLAGRYVLKNVLGSG
                     NMGTVYRATQLPMGREVAVKLMHSDLVRNPDLVARFEREAQAASTVDHPNAITVYDSG
                     KSPDGQVYIAMEFLDGETLSAVIERENRLPPDRALELWIPAVKAMVVAHRRGVIHRDL
                     KPDNIFVARRLSDDGSEEEVVKVLDFGIAKLLQGGKRASQTVVGARIGTAMYMSPEQL
                     EGREASPRSDVYALGLVLIEMLTGRLPWGKTGTETDQAVTMLRLINPPKRLRELCAGQ
                     VFSLELQKLLDEILAFEPATRPSDAGELIRRLAQVPESAPLALRGSRRTEGSQMFEAA
                     FLSANLKSELLADTPLTGTALPELAPHKGAGSADTPTPFLNPVPTVVTTKGVAEMLAP
                     GTPPSGNELPSVPTVVTGSRPDMPRFSPVTQSHTPVSSSQRFDVPRSASQRFEAPTPP
                     AGHDLPSVPTVVTPAMGARNSAPTALSPSLPLSALKPDPTTQPLPMARHPQSSDNNTI
                     RRVGSSDGGMRAELWHSSRARVSMVASVAVLLLIGLGSLFLSRRDTKAKPKFLQPDHN
                     ATENRLDHKPPMAIKPQVVIPTEYPLTVGFSRKRHPKVQVQCGPRMCFDNCQLGPGEL
                     CVAKMIGFKPRQYSYDELKPLAQAGRVEVEVRLNR"
     gene            3694..5064
                     /locus_tag="PKL17_17220"
     CDS             3694..5064
                     /locus_tag="PKL17_17220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46526.1"
                     /translation="MSQSEVSKVPKTGFVIEHSRPAEDASSSTLQWQAEPEKLALRPG
                     YTVGKCIGKGGVAEVFEAELRRPPLPPQRVALKRLLPGLRNDPAQQRKLRREAQIGAT
                     LSHDNIVRTLELIDLGDEVALALELVDGLPAQRLLRLLTEHGYRLRPPLVGHILHGIF
                     SALDYLQRLTPQQRPLVHADLSLENVMLARDGGVRLIDFGLSAETSGSDEEASGTDDV
                     LTALHEVGGKPSYLPPEGTTRIPTVTRDLYAAGVCAWELLTGRRFPVLPKGTTSRELG
                     SLVAFAATGQPAPVWLLLRDSLALLPTQRLRSAEEGLTRCAQLLRGHPTDAALARLVR
                     SLQTESPDWTPPEPLLPQLAPFDAAVDLVDRLRQSFVAAHVEVFAFDDASSGFLSRAK
                     KGQGRTLTAEPVLLDALQNGVASEDGVLCLRLRAAGGDRVVVIDPGEGRCYSPVTKTL
                     LTNILS"
     gene            complement(5124..5951)
                     /locus_tag="PKL17_17225"
     CDS             complement(5124..5951)
                     /locus_tag="PKL17_17225"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNK46527.1"
                     /translation="MRGLLLSAVLFVGCVVPAEPISDSPSDEDLLTPLLRSDLALPAD
                     AAGPDLAKAARCPQYMVLIPSSGTGFCIDQYESSTMRIIPTGGEEPWPFDRPVDGQTV
                     RAVIRPGQKPQAYISGAQAADACKRSGKRLCTETEWLSACQGPTKKTYPYGNTYQAGA
                     CNEGRATNPVNDCFGSGSSVFTYSNMNSPCCVSLPNTVATVGSYGKCVSDFGIYDMHG
                     NLHEWIDATTASGNGIFKGGFFVDAKINGSGCLYKTTAHAKTYHDYSTGFRCCQNPL"
     gene            complement(6018..6527)
                     /locus_tag="PKL17_17230"
     CDS             complement(6018..6527)
                     /locus_tag="PKL17_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46528.1"
                     /translation="MRTVILASLLLLLSSSARAQVVRVVVAPPAPQVEVRPAMPSPQH
                     IWIPGHWKWGGQRHVWMPGHWRHPPHPHHVWVEPRWAQEGGQWVYYEGRWNPTAQPVV
                     MQPAPQPVVVAPAPAVVVRPAPVVVAPAPAVVVRPAPVVVAPAPVVVPHGHGHGHGHG
                     HGWKHGRRW"
     gene            complement(6591..8405)
                     /locus_tag="PKL17_17235"
     CDS             complement(6591..8405)
                     /locus_tag="PKL17_17235"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK46529.1"
                     /translation="MFPLWNVLWTVLLLVTGTQATITPSKSETTLPAASIVCTSTADQ
                     VSFESVQKGLCPLKTQKAPADVWFRVTIPPTPEDILLFTSDLFTIKQGQLSLSVNHAP
                     PVSVRASDPLSQRPFVDGRLSIPVAKSPTESTEFLIKIRIRDQRQLAHPERHVFVQEK
                     TAAIHNQLTRWVLQGAFLGLVLIMAVYHLFLWRAERLAAAAWYSAALCALGLYFSINR
                     NLLPLLPWGSVPRLALLLHPPIAPVVGLCFLQFIRKYAHLPPRGEQLVKLSVRTLLVW
                     TPIALLIDAAGNAALAATSVNFLSVICMALTMGSLLVGAYRGDRESLVLAISSGPSVI
                     GISFLIGAMSGILPPGGLVDASMQIGVTTQVSLLGLALSDRIRRLRIDRDQAQAILRL
                     TLPDVIADRLKAGEKYIADRHSRVAVLFADLAGFTPLSATHEPETIVQLLDALFREMD
                     ALARKVGAEKIKTIGDCYMVVAGAPSPHADPVAALADLALAIPEATERVMKQVSHLSY
                     DLPERLSVRIGLHVGPVVAGVLGEQKLAYDLWGDTVNTASRMESHGVVGRVQCSQQVV
                     EALSDRYEFEPRGDITIRGKGTLPVWLLVGKRAKPQDS"
     gene            complement(8484..>9551)
                     /locus_tag="PKL17_17240"
     CDS             complement(8484..>9551)
                     /locus_tag="PKL17_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-dehydrocholesterol reductase"
                     /protein_id="HNK46530.1"
                     /translation="ELLLLRFLPGKLVYGPITAKGHVPKYVDNGVASFAMTVGLYLTC
                     SYVLHLFPASILYDNLGELLGSLNLFALLFCVVLYLKGRFAPSTPDHGLSGNPIFDYY
                     WGTELYPNLLGWDVKRMTNCRFGMMGWPLLLLSYAAKQHEVYGISDSMLVAVGIQLVY
                     IAKFFHWETGYLRTLDIMHDRAGFYICWGCLVWLPCIYTSGTMYLVNHPHNLGTPLAI
                     TLFALGVLAVLVNYLADEQRQRVRATSGHTTVWGKPPRLLIGHYTTEQGEKKDNILLA
                     SGYWGLARHFHYLPELAAALLWSLPALFDSALPYFYFVFLTILLVDRSYRDDERCAKK
                     YGTDWDEYRKLVPHRILPGIY"
BASE COUNT         1955 a         2792 c         3038 g         1766 t
ORIGIN      
        1 cagcagcagg gacttgtgtg ggttgccgac gctgagctca gcaaattttt gcggcagcgt
       61 caccccaaca tccgtcgcgt ctacagccaa ggtcagcccc gcagtgagac gcgggcggcg
      121 ggaaaagaag ctggtgagcg gctgatcttg cacaaaccaa tccacggacc tgcgacgaca
      181 gggcgcgcga caaagcttct gcccggacgc tgacgctcga cgggcgcgaa actgtatttt
      241 ctccggtgct cgaagccgcg tggcataatg aaaagcgctg tcttgggtct gccaagcagc
      301 ttccagctct gcgaacagcc acgatgagca aagacccgct agctcccgac aacttggcca
      361 atctgcgacg gactctccct ggacttggcg tttccgacgg cagcccgctg caagtacagg
      421 aagtctctgc cgccgacgtc gagcgagtcc tcgccgccac ccaaaccccc cccaatccaa
      481 atccatcacc cgcttcttca acgggagtgg ggctgggggg gcggagtgtg agtcagacgg
      541 ggattcctgt tttggctggg acgagcaatc cggcgattcc acagctgcgg gggacgagca
      601 atccggcgat tccacagctg cgggggacga gcaacccggc gattccgatg ttgcgcagta
      661 ccagtaactc ggcgatgccg ctgctgcgga cgacaggaaa tccggaaccg ccgattttgc
      721 cgcgggcttc ggaaacgagc attccatcgg tcccgccgtc atcgaatcgc ttgccgctgt
      781 tgattgctgg actgggcaca ctggcgctgg cgataggcgt cgtggcttgg cttcatcctg
      841 ggcagtctgc gacggggagc ggggttccag ccgacaaaac cgcggtgctt tcgctgccga
      901 cggcgttacc aaccggcggc tcgcaggcgg tggtgccggt gcggacggtg gctcagcctg
      961 ccccggtggt cgtcgtcgca ccagcaaagg acaagccgag cgacgacaag aaagaccaga
     1021 tttcggcgcg caaaccgccc gaagaaacag gccatgtggt gaccagttca aagccgacga
     1081 cttcgccgcc agcgactggg aatccagcca agccggttgc accgaaagcg gtggcgacca
     1141 aagtcgaagc caaaccgacg cgcgcgccgc agcttgatct atcgccggtg ccgcagctta
     1201 cgcgggacat tctgcgtcag cacatgacgg tggccgagcc caagtggcaa gcgtgtccca
     1261 aagacggcag cggcaaatac ttgaccatcg gaattgccgt cgctccatcg ggttccgtcg
     1321 tcaaagctga agtaatgggc cccgctggca gcacccagac tgcgcagtgc atcaccgagc
     1381 gaattcgtgg catgcgcttc cccgcctact ccgaaggggg cccaaagaac tttgtctgga
     1441 gctatcaggt tccgtagtcc gcggctgcgg acagcgcgct ggctgcaaag ccaaatcaca
     1501 tgacacccca taggccgtga tacgatgaaa aggctgagcc tgccgatcgc ctatgtaccg
     1561 tcgctgagcc tttttgcata gggacgcagc caagcccatt taaccgtagt ggccgtcgtg
     1621 aattcgcagc cgagccctgc catgttttgt gcctcgtgca agggaatctt tcccatcgag
     1681 gcaacggtct gcgatcgcga tggctcgccg ctcgaacgtg ccgaacaagt gctcgccggg
     1741 cgctatgtgc taaagaatgt cctcggatcg gggaacatgg gcacggtgta tcgggcgacg
     1801 cagctaccga tgggacgcga ggtcgcggtc aagctgatgc acagcgatct ggtccgcaac
     1861 ccggatctgg tcgcgcgttt cgagcgtgag gcccaagcgg cgtccacggt cgatcacccc
     1921 aacgcgatca ccgtctacga cagcggcaaa agtcccgacg ggcaagtcta catcgcgatg
     1981 gagtttctcg acggagaaac cctgtctgcc gtgatcgagc gtgaaaatcg tctgcctccc
     2041 gatcgggccc tggagctatg gattcccgcc gtcaaagcca tggtcgtcgc ccaccgtcgc
     2101 ggcgtgatac atcgcgacct gaagccggac aacatttttg tggcgcggcg gctgtcggac
     2161 gatgggtcgg aggaagaagt cgtcaaggtg ctcgactttg gcatcgcgaa gcttttgcaa
     2221 ggtggcaagc gggcgagcca gacggtggtg ggggcgcgga ttggcacggc gatgtacatg
     2281 tcgcccgagc agctcgaggg acgcgaggcg agtccgcgct cggatgtcta tgcgctgggg
     2341 cttgtgctga tcgagatgct gacggggcgg ctgccgtggg gcaagacggg caccgagacg
     2401 gatcaagcgg tcacgatgct gcggcttatc aatccgccaa agcgactgcg cgagttgtgc
     2461 gcggggcagg tgttttcgct cgaactgcaa aagctgctcg acgaaatttt ggcgttcgaa
     2521 ccggcgacgc gaccaagcga tgcgggcgag ctgattcggc ggctggcgca ggtgccggaa
     2581 tcggccccgc tggcactgcg cggatctcgg cggaccgagg gcagccagat gttcgaggcg
     2641 gcgtttctga gcgccaacct caagtccgaa ctgctagcgg acactccact gacggggacg
     2701 gccctgccgg aacttgcgcc gcacaaaggg gcgggaagcg cggacacgcc gacgccattt
     2761 ttgaatccag tgccgacggt ggtgacgacc aaaggtgtcg ccgaaatgct ggcacctgga
     2821 acgccgccaa gtggaaatga gctgccttcg gtgccgacgg tggtgaccgg gagccgaccg
     2881 gacatgccgc ggttttcgcc agtgacgcag tcacacacgc cggtttcgtc ttcgcagcgc
     2941 ttcgatgtac cacgcagtgc gtcgcagcgg tttgaagctc cgacaccgcc cgctggacat
     3001 gatttgccat cggtgccgac ggtggtgacg ccggcgatgg gagcgcgcaa ctcggctccg
     3061 acggcgctgt cgcccagctt gccgctgtcc gcgctgaagc ccgatccgac gacgcagcct
     3121 ttgcccatgg cgcgccatcc ccaatcgtcg gacaacaaca cgattcgcag agtcggcagc
     3181 tccgacggtg ggatgcgggc cgagctgtgg cacagctcgc gagcccgtgt ttcgatggtg
     3241 gcgtcggtcg cggtgctgct gctgattggg ctgggttcgc ttttcctgag ccgccgggac
     3301 accaaagcga agccgaagtt tctgcagcct gatcacaatg cgacggagaa ccggctggat
     3361 cacaaaccgc ccatggcgat caaaccccag gtggtcattc cgacggaata cccgctgaca
     3421 gttggctttt cacgcaaacg ccatccgaaa gtgcaggtgc agtgcggtcc gcggatgtgt
     3481 ttcgacaact gtcagctcgg tccgggcgag ctctgcgtcg ccaagatgat cggatttaag
     3541 ccgcgacagt acagttacga tgaactgaag cctctcgcgc aggctggccg cgtcgaagtc
     3601 gaggtgcggc tcaatcggta gtttgcgcat ggctggcact tgcccgccct tgcggctacg
     3661 ctcgcgtcct tgcttctgtg caaggagctg gctgtgtcgc aatcggaagt ctcgaaggtg
     3721 ccaaagacgg gttttgtcat cgagcactcg cgtcccgcgg aagacgcgag cagctcgacg
     3781 cttcagtggc aagcagagcc tgaaaagctg gcgcttcggc ccggatatac cgtcggaaag
     3841 tgcattggca aaggcggtgt ggcggaagtt ttcgaagccg agctgcgccg tccgccgctg
     3901 ccgccacagc gggtcgcgct gaaaaggctg ctgccggggc tgcgcaacga tccggcgcag
     3961 cagcgcaagc tcaggcggga agcgcagatc ggcgcaacgc tgtcccacga caacatcgtg
     4021 cgcacgctgg aacttatcga tctgggggat gaagtcgcgc tcgcactcga actggtcgac
     4081 ggactgcccg cgcagcgact tttgcggctg ttgaccgaac atggctatcg gctgcgacca
     4141 ccgctcgtcg gacacatctt gcatgggatt ttttcggcgc tcgactatct tcagcgcctg
     4201 acgccgcagc agcggccttt ggtccacgcc gacctgagcc tggaaaacgt gatgctggcg
     4261 cgcgacggcg gggtccgttt gatcgacttc ggtctgtcgg cagagacgag cggcagtgat
     4321 gaagaagcga gtggaaccga tgatgtcctg acggcgctgc acgaagtcgg gggcaagccg
     4381 tcgtatctac cgccggaagg aacgacgcgg attccgacgg tgacacgcga tttgtatgct
     4441 gccggcgtgt gcgcgtggga actgttgacg ggccggcgtt ttccggtgct gcccaaaggc
     4501 acgacaagcc gcgaactggg gagtctggtc gcatttgcag cgacgggaca gccggctccg
     4561 gtgtggctgc ttttgcgcga cagtctggcg cttttgccga cgcaaaggct gcggagtgcg
     4621 gaagaaggac tgacccgttg tgcgcagctt ctgcggggac atccgacgga tgcggcgctg
     4681 gccaggctgg tgcgctcgct gcagaccgaa agtcctgatt ggacgccgcc ggaaccgctt
     4741 ctgccgcagc ttgctccgtt tgatgcggcg gtggacttgg tggataggct gcgccagtcg
     4801 tttgtggcgg cgcacgtcga agtgttcgca ttcgatgatg cttcgtcggg cttcctaagc
     4861 cgcgcaaaga aagggcaggg gcggacgctc acggcggaac ctgtgctgct tgatgcgctg
     4921 caaaacggtg ttgccagcga agacggcgtt ctgtgtctgc gcttgcgggc tgcgggtggt
     4981 gatcgcgttg tggtgatcga tccgggcgaa ggtcgctgct attcacccgt gacaaagacg
     5041 ctgctgacca acatcctaag ctagacagag aatcagattt cccgtcgaga aatgactctg
     5101 tttgtgcgaa gtttgcagca gtcctacagc gggttttggc agcagcggaa acccgttgag
     5161 tagtcgtgat aggtcttggc gtgcgccgtg gttttgtaaa gacagccgct gccgttgatt
     5221 tttgcatcga cgaagaagcc gcctttgaaa atcccattgc cgctcgcggt ggtggcgtct
     5281 atccactcat gcaagttgcc gtgcatgtcg tagattccga agtcactgac acactttccg
     5341 taactgccga cggtggcaac agtgttcggg agactgacgc agcacggact gttcatgttg
     5401 gaataggtaa agacagaact tccgctgccg aagcagtcgt tgacgggatt ggtggcacgg
     5461 ccttcgttgc atgcgcccgc ctggtacgta ttcccatagg gataggtttt tttcgtcgga
     5521 ccctggcatg cagacagcca ctctgtttca gtgcacaggc gctttccgga acgcttgcag
     5581 gcatcggcgg cttgggctcc gcttatgtac gcttgtggtt tctgtccggg tcggatgact
     5641 gcccgaaccg tttgtccatc gacggggcga tcgaagggcc agggttcctc accgcccgtt
     5701 ggaataatac gcattgtgga tgattcatat tgatcaatgc aaaagccggt tccggaactg
     5761 ggaatcagca ccatgtactg cggacagcgc gccgccttgg ccaagtccgg tccagcggca
     5821 tcagcgggca gtgccaagtc agaccggagc agtggcgtca gcagatcctc gtctgaaggt
     5881 gagtccgaaa tcggttcagc cggaacgacg cagccgacaa aaagtaccgc ggacaaaagc
     5941 aaaccacgca tgcggcgcac catagcaccg gcatgctggg ttttctgcca gcggtatttg
     6001 gcagttatgc ggaaggatta ccagcgacga ccgtgtttcc agccgtgacc gtgtccgtga
     6061 ccgtgtccat gtccgtgcgg caccacaacg ggggcggggg caacgacgac gggcgcagga
     6121 cggacgacga ctgccggcgc gggggcaacg acgacgggcg cgggccggac gacaactgcc
     6181 ggcgcggggg cgacgacgac aggttggggg gcgggctgca tcaccacggg ctgcgccgtc
     6241 ggattccaac gaccttcgta gtagacccac tgaccgcctt cttgcgccca gcgcggctca
     6301 acccagacgt gatggggatg cggagggtgc cgccagtgac cgggcatcca aacgtggcgc
     6361 tggccgcccc acttccagtg acccggaatc cagatgtgtt gcggcgacgg catcgccgga
     6421 cggacttcga cttgcggtgc gggcggagcg acgacaacgc ggaccacttg ggccctggct
     6481 gacgacgaca acagaaggag cagagacgcc aaaatgactg tacgcatggc catcatctta
     6541 gcgcatttgg catccgcaca gtagcttcgg tgtatgttcc gctgccaagc ttaggaatcc
     6601 tgcggctttg cgcgcttgcc aacgagcaac cacaccggca gcgtgccttt gccgcggatc
     6661 gtgatgtctc cgcgcggctc aaattcgtac cggtcagaca gcgcttctac cacttgctgc
     6721 gagcactgga cgcgaccgac gacgccgtgg ctttccatgc ggctcgcggt gttgaccgta
     6781 tccccccaca gatcgtaggc cagcttctgt tccccgagca caccagcgac gacaggcccg
     6841 acgtgaagac caatgcgaac agacagccgc tccggaaggt cataggacag atggctgacc
     6901 tgcttcatga cgcgctcggt ggcttccgga atcgccagtg ccagatcggc cagcgcggcc
     6961 accggatcgg cgtgcggact tggagcgccc gcgacgacca tatagcagtc gccaatcgtt
     7021 ttgatttttt cggctccgac tttgcgggcc agcgcatcca tttcgcggaa cagcgcatcg
     7081 agtagctgca caatcgtttc cggttcatgg gtggcagaca gcggcgtaaa tccggccaag
     7141 tctgcaaaca acaccgcgac gcgggaatgc cgatcggcga tgtacttttc accggccttt
     7201 agcctgtctg caatgacgtc cggcaacgtc aaacggagta tcgcttgcgc ttggtcgcga
     7261 tcgatgcgca gacgacggat gcgatccgac agcgccagac cgagcagact cacttgagtc
     7321 gtgaccccaa tctgcatcga cgcatcgacg agtccgcccg gcggaaggat tccgctcatc
     7381 gcgccaatca ggaagctgat tccgatgacg gaaggacctg agctaatcgc caaaaccaga
     7441 gactcccgat cgccgcgata ggcgccaacc agcagcgaac ccatcgtcag cgccatgcag
     7501 atgacggaca ggaaattgac gctggttgcc gccagggccg cattgcctgc cgcatcgatc
     7561 agcagcgcga tcggagtcca cacaagcagc gtccgcaccg atagcttgac aagctgctcg
     7621 ccgcgcggcg gaaggtgtgc gtacttgcga atgaactgca aaaaacacag cccaaccacc
     7681 ggagcgatgg gaggatgtag gagaagcgcc agcctcggga cagaacccca cggcagaagt
     7741 ggaagcagat tgcgattgat cgaaaaatac agtccgagcg cgcacagcgc cgcgctgtac
     7801 cacgctgctg cggccagtcg ctcggctcgc cacagaaaca ggtgatacac cgccatgatc
     7861 agcacaagtc ccaggaatgc accttgtagc acccagcgcg tcagctgatt gtgtatggct
     7921 gcagtctttt cttggacaaa gacgtgccgt tccggatggg ccagctgcct ttggtcgcgg
     7981 attcggatct tgatcaggaa ttcggtgctt tccgtcggtg attttgcaac tggaatcgac
     8041 aggcggccat ctacaaaagg cctttgggaa agcggatcgg acgcgcgaac agagaccggc
     8101 ggcgcatggt ttacagacag cgacaactgt ccttgtttga tcgtaaacag atcacttgta
     8161 aacagcagga tgtcttccgg tgtcggagga atcgtgaccc ggaaccagac atccgcgggg
     8221 gctttctggg tctttagcgg gcacagtcct ttttgtaccg attcaaagga aacctgatcc
     8281 gcagtcgatg tgcagacgat cgaagccgct ggcagcgttg tctcggactt gctgggcgtg
     8341 atggtcgcct gtgttcctgt gacgagcagc aggaccgtcc acagcacatt ccacagagga
     8401 aacatccgct gggaattttc ctctatcggt ttggcaggtg tctagggatt tcgcgtcgga
     8461 agacggcggc ggacggggcg atgttagtag atgccaggca ggatgcggtg ggggacgagc
     8521 tttcggtatt catcccagtc ggtcccgtat tttttcgcgc agcgttcatc atcgcggtag
     8581 ctgcgatcga cgagcaggat ggtaaggaaa acaaagtaaa agtagggcag ggcggaatca
     8641 aacagcgccg gtagcgacca cagaagcgct gcagccagct cgggcagata gtggaagtgg
     8701 cgcgcaagtc cccagtatcc ggacgcgagc aggatgttat cctttttttc gccttgctcg
     8761 gtggtgtagt ggccaatcaa aagtcggggc ggcttgcccc acaccgtcgt gtgaccgctc
     8821 gtggcgcgga cccgctggcg ctgttcatcg gccaaataat ttaccagcac cgccagcacg
     8881 ccgagcgcaa acaacgtgat cgcgagcgga gtcccaaggt tgtgcggatg gttcaccaga
     8941 tacatcgttc ccgatgtgta aatgcacggc agccacacca aacatcccca gcaaatgtaa
     9001 aagcccgcac ggtcgtgcat gatgtcgagc gtgcgcagat agccagtttc ccagtggaag
     9061 aacttggcga tgtacacaag ctggattccg acggcaacca acatcgaatc actgattccg
     9121 tagacctcgt gttgtttggc ggcataggac aaaagcaaaa gcggccagcc catcattccg
     9181 aaccggcagt ttgtcatccg ctttacatcc cacccgagca gattgggata aagttcggtg
     9241 ccccagtagt aatcgaagat cggattccca gacagtccgt ggtccggcgt cgacggggca
     9301 aagcgacctt tcaggtacag aaccacgcaa aaaaggagcg caaacagatt cagacttccc
     9361 aacagctcgc ccaagttgtc gtacaaaatg ctggccggaa acaggtgcag gacgtaagaa
     9421 caggtcaggt acagaccgac ggtcatcgca aatgaagcga cgccgttgtc aacgtatttg
     9481 ggaacgtgtc ctttggccgt aattggtccg tacacaagct tgcccggaag gaagcggagg
     9541 agcagaagct c
//
LOCUS       DAORGN010000190         9537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3128_length_9537_cov_4.633094, whole genome shotgun sequence.
ACCESSION   DAORGN010000190 DAORGN010000000
VERSION     DAORGN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_9537_cov_4.633094"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /gene="murG"
                     /locus_tag="PKL17_17245"
     CDS             <1..1059
                     /gene="murG"
                     /locus_tag="PKL17_17245"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNK46531.1"
                     /translation="ARAIAEQLSAQGAQVTFVGTARGIEAKVVPQSGYPLELIDIAGL
                     KRQGLWLTLRSLLKLPLALFQTIGLLRRLSPSVVVGVGGYASGPVVLLAALLRIPTVV
                     LEQNSVPGVTNRILARFARVVYTAFPHAGRFFPRRKVSLLGNPVRKSITAENHDVAER
                     TGPVRLLVLGGSLGAKAVNTLVVDALADLAQQTPPGLSPLSLHLYVHHQTGASDLEAT
                     QARYGEHPTLKPWVRAEPFISDMATAYKDCDLMIGRAGATTIAELTAIGRPAVLIPFP
                     FAADDHQTHNARYLVENGAARMLQQKDTTPKLLASTIAELVLDRTVLQAMAAASRKLG
                     KPDAAQRISEEILRMATK"
     gene            complement(1063..2556)
                     /locus_tag="PKL17_17250"
     CDS             complement(1063..2556)
                     /locus_tag="PKL17_17250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK46532.1"
                     /translation="MDGRAGERERFFARLSLLLGCALLGVMLGGVAAKLRTSPLVSSV
                     DVSRSLLPDAGAGSLPPLEPTLDLSSPPSPPLADAGAPSQRELWALVVDSAGQPVEGV
                     SVLARPGLLTLSSATGNAASIGELGVLPGSLPFPDDVIAGQNVAAGRIERATASSSSV
                     WVVRSDRQGHAHFPSLPSGRLLLLANFSGKSASAEVEIPIVTPTSQAATALRVVLRLA
                     SPVECAESLSADASESLLGQPGMSTRTDLRGRVLDHRGFAVSSARIDATVGGSRQQIS
                     SDVRGQFVLPLLPSGDVRLSIRAPGFAPLVQTVKLEQRRDEVRFELRPGGGISGQVED
                     ARVSGLPDGLEVFLELPGGERLPIAVAPDGHFTETGVPTGTVVLRARARGYAAVQKVL
                     QVPEGSSPDQVTLRDVRLRFVRGAQLLGQVRLAGRSFEGADIQVQNEEGQLLARTRTD
                     RRGEFELADLPPGKLRVQASSAQGSASQTVELRPGDRPQIELELAGR"
     gene            complement(2602..3012)
                     /locus_tag="PKL17_17255"
     CDS             complement(2602..3012)
                     /locus_tag="PKL17_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNK46533.1"
                     /translation="MTARIALSLFALTFVLGCKGAKPDAPLKGNESLHDLMESLETAA
                     KPLFGRAPGAALTDADVPALQAAASRIQAINGAVKTRFASKQPPTFAAHSEQLEKGAQ
                     ELVAALGTKDAAAVQAAVKTIDGACGSCHKEFKQ"
     gene            complement(3025..3789)
                     /locus_tag="PKL17_17260"
     CDS             complement(3025..3789)
                     /locus_tag="PKL17_17260"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNK46534.1"
                     /translation="MSAIGLQTDSLGELTCDSILRGRLRLWQPVVGYRFSIDPLLLID
                     FVSETLSGQIADLGAGVGVVGLGLARRFERSQVTLVELQPRLAELSRRNITENLLTER
                     CQVVEADLLAPGTRKLLSGGGFDLVASCPPYYPVGHGGINPDSEEAIARHELRLPLPK
                     LVGAAKRLVGFRGRVAFVYPSPRLSELLVALHEHHLPVRRLRLVHPHVGEPAQRVMVE
                     AQKGYRGGLLIEPPLYIREADGRYTAQARRALGEPD"
     gene            complement(3793..5148)
                     /locus_tag="PKL17_17265"
     CDS             complement(3793..5148)
                     /locus_tag="PKL17_17265"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNK46535.1"
                     /translation="MAEVIPFRGLLYRPDVALGQSSTDASSLLAPPYDVIYEPERRNL
                     AGLSSFNSAHLILPQPPVGAQSDARYQVAAATLSAWQRAGVMTRDAQPAFYRYHQVFC
                     PPGEPNAAKIVRRGFIARIRLHRFSEGVVLPHERTLAGPKVDRLKLKRATRTHLSQVF
                     GLYDDPERKADAAFAAIESAEPALFGKTADGVEHHLWRLVDPDAIAEVQRVLRDRRIY
                     IADGHHRYETMLALREELRQDPAYCGLDSAVEYGSIFLCNVRDPGLFVFPTHRVLHSL
                     PGFDLPTLLARLGSAFSIEEKSVPTAAEAKEELQKRGQSSPSYLVLSRGRAFYLTLRP
                     EADLSILTGPKVLHSLDVTVLHALVLEQALGIDRTAQESQTYLRYVKDNEQALLASRE
                     ADVQAVFMMNPTRVTQVMEVSEAGEVMPQKSTYFYPKIASGIVLNPLDPAESVTREGA
                     F"
     gene            complement(5186..6055)
                     /locus_tag="PKL17_17270"
     CDS             complement(5186..6055)
                     /locus_tag="PKL17_17270"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNK46536.1"
                     /translation="MSKRVVLFGLSLLCGLVSVPSQSSWVATAAAASADGPKATLQRL
                     NGACEKLLRMKTEPGSSEETRVKQEIKKNASALLDYSELCKRALGEHWDKMGEPKRTE
                     FVATLRELIERNYIKQLRTNLDYEVSYGDESADGAEGKVATTLRLATKGKTTQVQIDY
                     RLIKQPDGSWKVYDVITDELSLVRNYRTQFSRIIGGNNYDGLLSRMKNKLSEEQSKDG
                     TAPAAAGTTEKKADAAAPADPKAAAPAKPGVTPATPAAAKPATPAAAKPANPAAAKPA
                     NPAAAKPATPATK"
     gene            complement(6052..7575)
                     /locus_tag="PKL17_17275"
     CDS             complement(6052..7575)
                     /locus_tag="PKL17_17275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK46537.1"
                     /translation="MQRREWKPVRPSLLLFLLLLLFAGRLPSARAQLKMPSDKEMEET
                     LAIARHAVGKPVDKAARKLDLPSLWSLAEGRFPGLLAARRNIESARHTRNEQIFLRLP
                     SADFSGFLTWSPNIKCKTAEEFALEGRPGELGGANHCTETNTAQTLNSSTIQNYLPIY
                     GALVQLGIRITQPLYTFGKLTAAWDLGQTGVDIASAQSEQARLDHQINVLKAYFGLKT
                     ARAALDTVSEGLDQVKKWVKQMDIDLEAGKGSASEIDLMRLKVAESQIELISIDLDRT
                     IASTLAALRYLVQDKSVDVDDENLLIWHDENEPLDYYLDAALRNRPELKVLRATGQGA
                     LLYRKLRIAELLPNFGLIVNFGYGYAGGVQDPNHGFMSRLNYLGAGIGLGMNWGLDVG
                     PKIARLQKAIADVNIFEARKRELLGGGALEIERAYNDVREAQRRLKTSETAERRARGW
                     LQGIKQSVDVGTAEGRDMNDALRTYFEQHISVLRATNDLNVACANLRRVSGLEVIPK"
     gene            complement(7782..8252)
                     /locus_tag="PKL17_17280"
     CDS             complement(7782..8252)
                     /locus_tag="PKL17_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016211370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNK46538.1"
                     /translation="MQLTQFTDYALRILLYLAHHPPVDGERLPAVLDVSRAYGISTNH
                     LSKVARLLVQNGFIKATRGRNGGLQLAKRPEEINLGEVVRRSEPHFDIVECFDMRTNT
                     CPIAGGCALKAVLMDARRQFLAVIDGKTLADVAGQRQRLVQLWKSPRGTSSSET"
     gene            8520..8903
                     /locus_tag="PKL17_17285"
     CDS             8520..8903
                     /locus_tag="PKL17_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNK46539.1"
                     /translation="MMQSLYDQLGGAPAISAVVESFYRKMLSDDRVSRFFDGVDMDRQ
                     MAKQTAFITMVTGGPNHYTGRDMRTGHAHLVSRGLADLHVDVVVQHLAATLAEFGVSE
                     ENIGKVRAVCESVRDDVLGRQKPQQ"
     gene            8958..>9537
                     /locus_tag="PKL17_17290"
     CDS             8958..>9537
                     /locus_tag="PKL17_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNK46540.1"
                     /translation="MQFSLALSHAAQLIAQGQRFLIGAHSRLDGDALGSMLVSAHGLR
                     QLGKEVYLYNPDPVPRRLAWLPGAQDVRRKVPGGVRFDATLIHDCGARHLLGDHFPDG
                     KVTGPLIVLDHHEIVSDLGDVVLRDSTAGSAGVIAMRLLSELGVAENDLPTPLATALF
                     VSLVEDTGWFRYPNSNPEVFRLAQAAIAAGVKT"
BASE COUNT         1897 a         3003 c         2779 g         1858 t
ORIGIN      
        1 gcccgcgcca tcgccgagca gctcagcgca caaggggccc aggtcacgtt tgtcggcacg
       61 gcgcgcggaa tcgaagccaa agtggtgccg cagtcgggtt atccgctcga gctcatcgac
      121 atcgcggggc tgaagcggca gggactttgg ctgacactgc gctcgctttt gaagctgcct
      181 ttggcgctgt ttcagacgat tggtcttttg cgtcgcctgt cgccgagcgt ggtcgtcgga
      241 gtgggtggtt atgcgtctgg ccccgtcgtg ctgctcgcgg cgctgctgcg aattccgacg
      301 gtggtgctcg aacaaaactc ggtgccgggc gtgacgaatc gcattttggc gcgctttgct
      361 cgggtggttt acaccgcatt tccgcacgca ggtcgattct ttcctcgacg gaaagtgtcg
      421 ctgctgggca atccggtgcg caaatccatc acggcggaaa accatgatgt ggccgaacga
      481 accgggccgg tgcgactgct cgtgctcggt ggttcgctag gggccaaggc agtcaacacg
      541 ctggtcgtcg atgcgctggc cgatttggcg cagcagacac cgccgggttt gtcgccgctg
      601 tctctgcacc tgtacgtcca tcatcagacg ggcgcatccg acttggaagc aacgcaggcg
      661 cgatatggcg aacacccgac gctaaaaccc tgggtccggg ccgagccatt catcagcgac
      721 atggcgacgg cgtacaaaga ctgtgatctg atgattggcc gggccggcgc gacgacgatt
      781 gctgaactga cggcgattgg tcggccagcg gtgctgattc cgttcccgtt cgcggcggac
      841 gatcaccaga cgcacaacgc acggtatctg gtcgaaaacg gcgcggcgcg aatgctccag
      901 caaaaagaca cgacgcccaa gctgctcgcc agcaccatcg ccgagctggt gttggatcgc
      961 acggtcctgc aagcgatggc cgctgcgagc cgcaagctag gcaagcccga tgcagcgcag
     1021 cggatcagcg aagagatcct gcgcatggcg acgaaataag cgctaccgtc cggcgagttc
     1081 tagctcaatc tggggccgat cgccgggacg cagctcgacg gtctgtgacg cacttccttg
     1141 cgcgctactg gcctgcacac gcagcttgcc cggcggaaga tcggcaagtt caaactctcc
     1201 ccgccgatcg gttcgcgtcc gtgccaaaag ctgcccttct tcgttctgca cctggatgtc
     1261 tgcgccctcg aagctgcggc ccgcaagacg cacttggcca agcagctggg caccgcggac
     1321 aaagcgcaag cggacatccc gcaaagtgac ttgatcgggc gacgagcctt ccggcacctg
     1381 aagcactttt tgcaccgcag catagccacg cgcgcgcgcc cgaagcacga cggttcccgt
     1441 tggaacgcca gtctcggtga agtgcccatc gggagccacc gcaatgggca ggcgttcccc
     1501 gccgggcagt tccaaaaata cctccagtcc atcgggaagc ccgctgactc tggcatcttc
     1561 tacttgccct gaaatgccgc cgcccggacg cagctcgaag cgcacttcat cgcgtcgctg
     1621 ttccagcttc accgtctgca caagcggtgc aaaaccgggc gcacgaatcg aaagccggac
     1681 gtcccccgat ggcagcagcg gcagcacgaa ctgaccgcga acatcactgc tgatctgctg
     1741 acggctgcca ccgacggtcg catcgatccg cgccgacgac accgcaaagc cccgatgatc
     1801 caagacacgc ccgcgcagat ccgttcgcgt cgacataccc ggctgaccca acagcgactc
     1861 acttgcatca gcgctcagac tctcggcaca ctcgacgggg ctcgcaagcc gcagcaccac
     1921 ccgcagcgct gtggcggctt gggaagtggg cgtcacaatc ggaatttcga cctctgctga
     1981 cgcgctcttg ccggaaaagt tggccaaaag cagcagacgc ccacttggca gcgacggaaa
     2041 gtgagcatga ccctggcgat cactgcgcac cacccaaacc gatgacgacg acgccgtcgc
     2101 gcgttcaatc cgacccgccg cgacattctg accggcgatg acatcatccg gaaacggcag
     2161 cgaaccgggc aggaccccaa gctcaccgat gctggcagcg tttcccgtcg cggatgacag
     2221 cgtcaaaagc cccggtcgcg ccagcaccga aacaccttcg acgggctgac cggcagaatc
     2281 caccaccaaa gcccacagct ctcgctgcga aggtgctccc gcatcggcaa gcggcggcga
     2341 tggcggcgat gacagatcga gtgttggctc aagcggcggc agacttcccg caccagcatc
     2401 tggcagcaat gatcgcgaca catcgacgga agacaccagt ggtgaggtcc gcagcttggc
     2461 cgcaacccca cccagcatca ccccgagcag cgcgcagccc aacagcagtg agagtctcgc
     2521 aaaaaatcgc tctcgctcgc ccgctcgccc gtccatcgtc tccccaggct actacgatcc
     2581 gcttcggtcg gctccgccag actactgttt gaattccttg tggcaagaac cgcacgcgcc
     2641 gtcgatggtc ttgaccgccg cttgcaccgc cgctgcgtcc ttggttccca gcgccgcgac
     2701 aagttcttgc gcgccttttt caagctgctc actgtgcgcg gcaaatgtcg gtggctgctt
     2761 gctcgcaaag cgggttttca ccgcgccgtt tattgcctgg atgcgactgg ccgccgcttg
     2821 cagagcaggc acgtctgcat cggtcagcgc agcccccgga gcccgaccaa acagcggctt
     2881 ggccgcggtt tccagcgatt ccatcaggtc gtgaagcgac tcattgcctt tgagcggcgc
     2941 atcaggcttg gcacctttgc agccgagaac aaacgtcaga gcaaagagcg aaagagcaat
     3001 ccgagcggtc atggtttctc ctgtttagtc cggttctccg agcgcgcgtc gggcctgcgc
     3061 agtgtagcgt ccgtcggcct cgcgaatgta gagcggtggc tcgataagaa gcccgccacg
     3121 gtagcctttc tgtgcttcga ccatcacccg ctgcgcaggc tcaccgacgt ggggatggac
     3181 aagccgcagc ctccgcaccg gcaagtgatg ttcatgcagg gcgaccagca gttccgacag
     3241 ccgcggcgac gggtacacaa acgcaacccg cccacgaaag ccgaccagac gcttggccgc
     3301 gcccaccagc ttgggcagcg gcagacgcag ctcgtgacga gcaatcgctt cctcgctatc
     3361 gggattgatg ccgccatgcc ccaccggata gtaaggcgga cacgacgcca ccaagtcgaa
     3421 gccaccgccg gaaagcagct tccgcgtccc aggcgccagc agatcggctt cgaccacttg
     3481 gcagcgctcg gtcagcagat tttccgtgat gttgcgtcga gacagctccg ccagccgcgg
     3541 ctgaagctca acgagtgtca cttgggatcg ctcaaaccgc cgcgcgagcc ctagcccgac
     3601 cactccgacg cccgctccca aatccgcaat ctgacccgaa agcgtttccg acacaaagtc
     3661 gatgagcaag agaggatcga tgctgaagcg atatccaacg acgggctgcc acaaccgcag
     3721 ccgcccgcgc agaatcgaat cgcaggtcag ttctccgaga gaatccgtct gaaggccaat
     3781 cgccgacaca ggctagaacg ccccttcgcg cgtcaccgat tctgccgggt cgaggggatt
     3841 tagcacgatg cccgacgcta tctttggata gaagtaggtc gatttctgcg gcatcacttc
     3901 gcccgcttcc gacacttcca tcacctgcgt gacgcgcgtg ggattcatca tgaacaccgc
     3961 ttgcacatcg gcttcccgcg acgccaaaag cgcttgttcg ttgtccttca catagcgcag
     4021 ataggtctgc gattcctgcg ctgtccgatc aattcccagc gcttgttcca gcacaagcgc
     4081 atgcagcacc gtcacatcca agctgtgcag cacttttgga cccgtcaaaa tcgacaagtc
     4141 cgcttcggga cgtagcgtca gatagaacgc acgtccgcgc gacagcacaa gatagcttgg
     4201 cgacgactga ccgcgctttt gcagctcttc cttggcttcg gcggcagtag gaacgctctt
     4261 ttcctcgatc gaaaaagcac tcccgagccg tgcaagcagc gtcggcaaat cgaatcctgg
     4321 caggctgtgc agcacccgat gggtcggaaa cacaaacaga cctggatcgc gcacattgca
     4381 aaggaaaatc gatccgtatt ccaccgcgga atccagacca cagtaggctg ggtcttggcg
     4441 cagctcctcg cgcagcgcga gcatcgtttc atagcgatgg tgtccgtcgg caatgtagat
     4501 gcgacggtcg cgcagcacgc gctgcacttc agcaatcgca tccgggtcaa caagacgcca
     4561 cagatggtgc tcgactccgt ccgcagtctt gccgaaaagc gcaggttctg ccgattcgat
     4621 cgcagcaaac gcagcatcgg ctttgcgttc gggatcgtcg tacagaccaa agacctgtga
     4681 cagatgggtt cgcgtcgccc gcttgagctt gagccgatcc actttcggac ccgctagcgt
     4741 gcgctcatgc ggcaggacaa caccttcgga aaaacgatgc agccgaatgc gcgcgataaa
     4801 cccgcggcga acgatcttgg cggcgtttgg ctcacccggc ggacaaaaca cctggtggta
     4861 gcgataaaac gccggctgcg cgtcgcgcgt catgacaccg gcccgctgcc acgcagaaag
     4921 cgtcgctgcc gcgacttgat accgcgcatc ggactgagcc ccgaccggcg gctgcggcag
     4981 gatcaagtgt gcgctgttaa acgaagacag acccgccagg ttgcgccgtt ctggttcata
     5041 aatcacgtca tagggcggtg ccagcagcga cgaggcatcc gtggatgact gcccgagcgc
     5101 cacatccgga cgatacagca gaccacgaaa cggaatcact tcggccatcg caggttcctt
     5161 tcttcttggt gaacggatcg ccagactact tggtcgccgg ggtcgccggt ttcgccgctg
     5221 ccgggttcgc cggttttgcc gctgccgggt tcgctggttt cgccgctgcc ggggtcgctg
     5281 gtttcgctgc tgctggggtc gctggtgtca cgccgggctt ggctggagcc gcggctttgg
     5341 ggtccgcagg agccgctgca tcagcctttt tttccgtcgt ccctgctgct gcgggtgccg
     5401 taccgtcttt ggactgttct tccgacagct tgtttttcat ccgcgagagc agaccgtcat
     5461 agttgttgcc accgatgatg cgcgaaaact gcgttcggta gttgcgaacc agcgacagct
     5521 catcggtgat cacgtcgtag actttccaac tgccatcggg ctgtttgatc agtcgatagt
     5581 caatttgaac ctgcgtggtc ttgcccttgg tcgcgagccg cagcgtcgtc gcaactttgc
     5641 cttccgcacc gtcggcactc tcatctccat aggagacttc gtagtccagg ttggtccgca
     5701 gttgcttgat gtaatttcgc tcgataagct cgcgcagcgt cgcgacaaac tcggtccgct
     5761 tgggctcgcc catcttgtcc cagtgctcac cgagcgcccg cttgcacagc tcggagtagt
     5821 cgagcagcgc ggacgcattc tttttgatct cttgcttgac gcgcgtttcc tcggaagatc
     5881 ccggctctgt cttcatgcgc agcagctttt cgcacgcgcc attgagccgc tgcagcgtcg
     5941 ctttgggacc atccgccgaa gccgccgctg ccgtcgcaac ccacgaggac tgcgagggaa
     6001 cagaaaccaa gccgcacaaa agagacagac cgaacagaac cactcgtttg ctcatttggg
     6061 aatgacctcg agaccggaaa cgcgacggag gtttgcacac gcaacattca gatcgttggt
     6121 cgcccgcagc accgaaatgt gctgctcgaa gtatgtacgc agcgcatcgt tcatgtcgcg
     6181 accttcagct gttcctacgt cgaccgattg tttaataccc tggagccacc cgcgggcccg
     6241 tcgctccgcg gtctccgatg tctttagacg gcgctgggcc tcccgaacat cgttgtacgc
     6301 gcgctcaatt tccagggcac cgccccccag aagctcacgc ttgcgcgctt cgaagatgtt
     6361 cacgtcggca atcgcctttt gcagccgtgc gatcttcggc ccgacatcaa gtccccagtt
     6421 catacccaga ccgatgccag cccccaggta attgagccgg ctcatgaagc catggtttgg
     6481 gtcctgtacg ccgcccgcat agccgtagcc aaagttcacg atcaggccaa agtttggcaa
     6541 aagctcggcg atgcgcagct tgcggtaaag gagcgcgccc tgccccgtcg cccgcagcac
     6601 cttcagttct ggacgattgc gcagcgccgc gtcgagatag tagtcgagcg gttcgttttc
     6661 atcatgccag atgagcaggt tttcatcgtc gacatcgacc gacttgtcct gcacaagata
     6721 gcgcagcgcc gccagggtcg atgcgatcgt gcgatccaga tcgatgctga tcagttcaat
     6781 ttgcgattct gcgactttga gccgcatcag gtcgatttcc gacgcagagc ctttgcctgc
     6841 ttcgagatcg atgtccatct gcttgaccca ctttttgacc tgatcgagcc cctcgctgac
     6901 cgtatcgagc gctgcccgcg cggtcttcag tccaaagtag gctttgagta cgttgatctg
     6961 gtgatcgagc cgcgcctgtt cagactgcgc cgacgcaatg tcgacgccgg tctgtcccaa
     7021 gtcccacgcc gccgtcagtt tcccgaacgt ataaagcggc tgcgtaatcc gaatgcctag
     7081 ctgaaccagc gcgccataga tcggcaggta gttctgaatc gtcgacgagt tcagcgtctg
     7141 tgcggtgttg gtctcggtgc agtggttcgc gccaccgagc tcgccaggcc gaccttctag
     7201 cgcaaattcc tcggcggtct tgcacttgat gttgggcgac caagtaagaa agcccgaaaa
     7261 atccgccgac ggcagccgca ggaaaatctg ttcgtttcgg gtgtggcgcg ccgattcaat
     7321 gttgcgacgc gcagccaaaa gacccggaaa gcggccttcc gcaagtgacc acaaagatgg
     7381 aaggtccagc ttgcgggcgg ctttgtcgac tggctttcca acagcgtgtc tggcgatcgc
     7441 cagcgtctct tccatttcct tgtcggacgg catcttgagc tgcgcgcgcg cgctcgggag
     7501 tcgcccggca aacagcagaa gcaagagaaa cagcagcaac gaaggccgca caggtttcca
     7561 ctctcgacgc tgcatgagag cgaagatagg aagagagaga gttggcacga gcaagctcca
     7621 caaagccaag aggtaagggc tttggtatta gtccgagcag gtcactcgtg caagggtgaa
     7681 acaggcaaaa aaatgcgcag ctacaacagt tttccgagga gaagactgac tgggagcgaa
     7741 acgcaaaaaa gcgaaacgct gaaccgattc tggtgacgcg cttacgtttc cgaactggat
     7801 gttccgcgcg gacttttcca aagctggacc aaccgctgcc gctgtccggc cacatcagcg
     7861 agcgtcttgc cgtctatcac ggccagaaac tgccgtcgcg catccatcag caccgctttg
     7921 agcgcgcagc cacccgctat cgggcaggtg ttggttcgca tgtcaaaaca ctcgacgatg
     7981 tcgaagtgcg gttcgctgcg gcggaccact tcaccaaggt ttatctcttc ggggcgcttg
     8041 gcgagctgaa gtccgccatt gcggcctcgc gtcgctttga taaagccgtt ttgcaccagc
     8101 agccgcgcca ctttgctcag gtgattggtg gagatgccgt aggcacggct cacgtcgagt
     8161 acagcaggaa gtcgctctcc gtctaccggc gggtgatggg ccaagtaaag caggatacgg
     8221 agtgcgtaat cagtaaactg ggttaattgc atcgctcata cctcagccac gacaatgctg
     8281 tgcggcaggt gtcttctctg ttcgtagcac aaaaccgatt ccggacacgc cgacgatcga
     8341 gatgaacttt cttcatatcg accgactgct ttttcggcaa gtgtttgcag cgaaaaggtc
     8401 cgcaaaaaat ggggaagcaa gcacaaggta caggcgcaac agcagccatc cgacggagtt
     8461 cgcaatttgc gcactttgaa tacatgtttt tatgttacgt tacgctttcg gaggtttcga
     8521 tcatgcagag cctgtacgac caacttgggg gcgcccccgc catttccgct gttgtagaga
     8581 gtttctatcg taagatgctg tccgatgacc gagtgtcgcg tttctttgat ggcgtcgaca
     8641 tggatcgcca aatggcgaaa caaactgcgt tcatcacgat ggtgaccggc ggcccgaacc
     8701 actacaccgg gcgcgacatg cgaaccggac acgctcacct cgtcagccga ggtcttgcgg
     8761 atcttcacgt cgatgtggtg gtccagcacc tcgccgccac gctcgccgag tttggcgtca
     8821 gcgaagaaaa catcggcaaa gtccgcgccg tctgtgaatc ggttcgagac gatgtgctag
     8881 gccgccaaaa gccacagcag tagcagccct tttctttgtt tgttttcgtc gcggcgcccc
     8941 ctgcctatac tcgccgcatg cagttttccc tcgctctgtc ccacgccgcg cagcttattg
     9001 cccaaggcca gcgtttcctg atcggtgccc actcacgact cgacggagac gcgctcggat
     9061 cgatgctggt gagcgcgcat ggtctgcggc agttgggaaa ggaagtctat ctgtacaatc
     9121 ccgatccggt gccgcgacga ctggcgtggc tgccgggtgc gcaagatgtg cggcgcaaag
     9181 taccgggtgg cgttcgtttt gatgcaacgc tgatccatga ctgtggcgcg cgacacctgc
     9241 tcggagatca ttttcccgac ggcaaagtca ccgggcccct gatcgttctc gatcatcatg
     9301 agatcgtgtc cgatctgggc gatgtggttt tgcgcgacag caccgccgga tcagcggggg
     9361 tgattgcgat gcgactcctt tccgagcttg gggtggcgga aaacgacttg ccgactccgc
     9421 tcgcgacggc gctttttgtg tcgctggtgg aagacactgg ctggtttcgc tatccgaaca
     9481 gcaatccgga agtatttcgg cttgcgcagg cggcgatcgc agccggcgtc aagacgt
//
LOCUS       DAORGN010000191         9531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3131_length_9531_cov_5.231321, whole genome shotgun sequence.
ACCESSION   DAORGN010000191 DAORGN010000000
VERSION     DAORGN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_9531_cov_5.231321"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKL17_17295"
     CDS             <1..352
                     /locus_tag="PKL17_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK46541.1"
                     /translation="EDALTRLYAYNWPGNVRELENVISQAMVFCEGTTISAEALPAHV
                     RGEDVARPGGTDLASLDLPSGGMALPEILDDLERQLILKAYREAKGVKTETARLLGIK
                     PSALYYKLDKYRIA"
     gene            complement(355..1110)
                     /gene="fabG"
                     /locus_tag="PKL17_17300"
     CDS             complement(355..1110)
                     /gene="fabG"
                     /locus_tag="PKL17_17300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNK46542.1"
                     /translation="MSQAPSLRRVALVTGASRGIGRAIAKALVRDGLFVVANYVANEA
                     AAQQTLTELGEGNAAIAKFDVSDEAAVDAAVKSIGDTYGRLDVVVNNAAVAVDGLLMR
                     VKTADWHKVLETNLSSAFYLSRAASRLLLKAKESGRIINLTSVVGEMGNTGQVSYVAA
                     KAGLIGMTRTLARELASRGITVNAVSPGFIETDMTAQHVQGEARQKLIAQIPLGRIGA
                     AEDVAEAVAFLASPRASYITGQVLRVNGGLLIG"
     gene            complement(1121..2083)
                     /gene="fabD"
                     /locus_tag="PKL17_17305"
     CDS             complement(1121..2083)
                     /gene="fabD"
                     /locus_tag="PKL17_17305"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNK46543.1"
                     /translation="MHNIAFVFPGQGSQHIGMGRALFDAFPYVRSLFDQADAALGYSL
                     STLCFEGPDSELVRTENTQPALLTCSVAAAEVLRRELALAPALCAGHSLGEYSALVAA
                     DSIDFADAVRLVHLRGRFMQEAVPQGLGAMAALVGLSSDDVAQLCVEASTEEEKVQPA
                     NDNGAGQVVISGHKPAVDRVMAQAKAKGCKLVKALPVSAPFHSALMQPAAAKLAESLG
                     SITLRAPRITVVANIDAEPYPQGDVSAVRDRLVRQVAGTVRWEGCVKKLASLGATAIV
                     EAGPGKVLQGLVKRIVPHVALAGFADPSGLAAVAELASTKPSQP"
     gene            complement(2093..3094)
                     /locus_tag="PKL17_17310"
     CDS             complement(2093..3094)
                     /locus_tag="PKL17_17310"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNK46544.1"
                     /translation="MRRIRIAGTGRAVPDRVLTNDDLSKMVDTSDAWIRERTGISVRH
                     TVRRGEEAASDLAARAGTHACEAAGISPKEIDAVICSTISPDMPLPSCAVYVQRKVGA
                     APTSAAFDLSAACGGFIYGLAVAEGLLRSGLYRNILLCGVEVLSGYVNWQDRNTCVLF
                     GDGAGAAILRLCTEEEHEAGLGVQSVHLFADGTQAEALMIPGGGSLYPTSAQTLADGK
                     HFIHMNGKVIFTNAVRNLSSSCLTALAHHGLTPKDVDLVIAHQANLRIVEAVAQRLDL
                     PMDKFFINIDRYGNTSSASVPIALDEAVRMGKVKRGDRLLFCALGAGLAWGATVVRF"
     gene            complement(3174..4280)
                     /gene="plsX"
                     /locus_tag="PKL17_17315"
     CDS             complement(3174..4280)
                     /gene="plsX"
                     /locus_tag="PKL17_17315"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNK46545.1"
                     /translation="MAPVIAIDAMGGDTAPGPEVEGAISAVRERGARVILLGDESRLR
                     AELDRLGGAHLGIPIRHCTEVITMDDHPGQAVRKKRQSSMRIGFELVKSGEAAAVVSA
                     GNSGAMLACGLFVLGRMSGLDRPGIAVTMPTLVPGLFGSPPRIGECVLLDTGANVDVR
                     PQTLAQFAILGAQFAKLRASSPRLRPRVGLLSNGEEASKGTPILRETHALLTAHPSSA
                     FDYVGYVEGRDLFQFRRTANTALRDEGLDVVVTDGFTGNVVLKTAEGAGRFLADLLRS
                     EVKRSLLAQLGALLMMPALKSLRRVVDVEGRGGAPLLGVNGVAIICHGRASARAIARA
                     VQVAEEHVQSGLIEATQAALRAHRFGDGTASSDP"
     gene            complement(4300..4521)
                     /gene="rpmF"
                     /locus_tag="PKL17_17320"
     CDS             complement(4300..4521)
                     /gene="rpmF"
                     /locus_tag="PKL17_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNK46546.1"
                     /translation="MAVQKRRQSSARRDSRRAQWMKMATPASSVCPNCQAAKMPHRIC
                     EACGWYGPAKEGRVVIPQPTENEAAAEKA"
     gene            complement(4559..5110)
                     /locus_tag="PKL17_17325"
     CDS             complement(4559..5110)
                     /locus_tag="PKL17_17325"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNK46547.1"
                     /translation="MTDLSFRLKDLENKERHIDCVLPADMILDALADMDIDVSQSSLR
                     LVADVTKHNVTVLCDGKLTGELLLPCQLCLGPAHVVVDQRLHTVFVPPSSALGSSDGE
                     ASDEADDDVDDVDYAHHDGETVDLAPSLREFIILAVPITVHCKEDCRGLCPLCGADKN
                     QGECSCQPVGKPSPFSALRDVKL"
     gene            5217..6194
                     /locus_tag="PKL17_17330"
     CDS             5217..6194
                     /locus_tag="PKL17_17330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNK46548.1"
                     /translation="MPSFVVTQEDDDKTLAAVVRQHLGVAWTAARTMCERHKVQIDGQ
                     LAPDPVRRVRLGQTITVNQTAPRPMPQAVAAAQKRVVYEDSQLIVINKPVGMSSVPYE
                     RGETSTAMDMVRDAWRASGRNATEQPLYVVHRIDKDTSGLLMFAKTRTAERDLQQLFR
                     HHDIRRRYICVVHGYFLSQTIETYLIKDRGDGLRGSLAPWKAELGHAGRLALTHVEVL
                     EHIGTLATVCAVQIETGKTHQIRIHLAEAGHPIIGERVYIRDFTRKGQTALPCPRLLL
                     HAETLGFEHPNTGDDLMFQEEPPTDFADAVARLRAQARKERQQAEAAAR"
     gene            complement(6205..6717)
                     /locus_tag="PKL17_17335"
     CDS             complement(6205..6717)
                     /locus_tag="PKL17_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNK46549.1"
                     /translation="MDRLWAPWRMAYILQASGEQGAQQCIFCQFPSEGPARFAEHLIL
                     CCNRSAFAIMNRFPYGSGHIMVVPRRHGADLMALPADEYQATCELLRQANQIVGKELG
                     AHGANLGMNLGRVAGAGIDQHAHFHIVPRWNGDTNFMPVVGDVKVISEHLQATYDKLR
                     PAFAHLDKLD"
     gene            complement(6740..7882)
                     /locus_tag="PKL17_17340"
     CDS             complement(6740..7882)
                     /locus_tag="PKL17_17340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46550.1"
                     /translation="MTTPLRSPDSNTQLIGQRVGEYRVLREVGRGGMGVVFEAVHNET
                     GQQVAVKAIHESLLTNLSAVQRFVAEARLMGRVDHPGLTTVFDSGQLPGGRPYFVMEF
                     LVGESLRERLRSKGRLGQKEAVAIGLSIAEALAAAHAAGIVHRDLKPDNLLLIDDASA
                     PFGFRVKILDFGIAKAIAALAGETLPGVQTATGLLLGTPTYMAPEQCRGGVEVTDRAD
                     VYALGIVLYQLLSGAPPFVSRSPGDVIAMHIVEPVPPLSEKAPDVARPIIALVDTMLR
                     KKAAERPTMAEVATRLHALRNLEPGTVILPPSQAAAKISDAMQAMPTTEMRANETRLF
                     AERSTSPAPAVVQAPVPAKTERSSWLIVALLCAVILGGVTAALLLR"
     gene            complement(7970..>9531)
                     /locus_tag="PKL17_17345"
     CDS             complement(7970..>9531)
                     /locus_tag="PKL17_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK46551.1"
                     /translation="AKNLFLASVMLAVACGNETPMDQVLDPGGPQVDVQPTAPVTDVG
                     GDPNSLAMAKSNGAMIGLGTSDTLQESAVVTDQLGQTHTHYNRTYSNLPVHGGDLVLH
                     QKGSTFLGFSSTLKSPVSVALQPNISAISATAEALRHYTGTAVLAKVSLVVHAFGDSP
                     KLAYDVVIYNDEVKDDFASIQHTYVDALTGAYLSRHNEVKEAAAVGSGKSLYMGTLSL
                     TANSITGGFELRDPDRGGGYTTNMNKKTSGNGTIFTDLDNVWGNGTAADLASAAVDAH
                     VGVALTWDYYKNVHGRNGIANDGKGSYNRVHYGRAYNNAFWSDSCFCMTYGDGDGKSF
                     STLVSLDVAGHEMSHGVTARTAALVYSGESGGLNESTSDIFGTMVEYYANNAADAGDY
                     LIGEKITLYGNRWLRNMADPRADGYSIDHYSQMTSSLDVHYSSGLANKFFHMLAEGGT
                     NKTSGQSVTGIGRGKAEKIWYRALTVYMTSNTNFKGARTATISAASDLYGASSAEATT
                     VAAAWTACGVN"
BASE COUNT         1902 a         2954 c         2931 g         1744 t
ORIGIN      
        1 agaggatgcc ttgacgcggc tttatgccta caactggccc ggcaacgtcc gcgaactgga
       61 aaacgtgatt tcgcaagcaa tggtgttctg cgagggaacg accatcagcg cagaggcact
      121 gccggcccac gttcgcggcg aagatgtcgc aaggccagga ggcaccgact tggcatcgct
      181 tgacctgccg agtggtggca tggcgctgcc cgagatcctc gacgacctgg agcggcagct
      241 catcctaaaa gcgtatcgag aggcaaaggg cgtaaagacg gagacagcgc gccttctggg
      301 gatcaagcca agcgcgctgt actacaagct cgacaagtat cggattgcgt agcgtcagcc
      361 gatgagcagg ccgccgttga cacgcaggac ttggccggtg atgtagctgg cacgcggcga
      421 agcaaggaat gcaaccgctt cggcgacgtc ttccgcggcg ccgatccgtc caagcggaat
      481 ctgggcaatc agcttctgcc gggcttcgcc ctggacatgc tgagcggtca tgtcggtttc
      541 gataaaaccc ggcgacacag cgttcacggt gattccacgc gacgccagct cgcgcgccag
      601 agttcgcgtc atgccgataa gaccagcctt ggccgcgacg tagctgacct ggccggtgtt
      661 gcccatctca cccacgacgg acgtcaggtt tatgatgcgg cccgactctt tggccttgag
      721 cagaagacgc gacgccgccc ggcttagata gaacgccgac gacaagttcg tttctagtac
      781 tttgtgccag tccgccgtct tgacccgcat gagcagtcca tcgaccgcaa cggcggcgtt
      841 gttgacgacg acatcgagcc gcccgtaggt gtcaccgatc gacttgaccg ccgcatcgac
      901 cgctgcttca tcggaaacat cgaacttggc gatcgccgca ttgccttcgc ctagctcggt
      961 cagcgtctgc tgggctgcgg cttcattggc cacgtaattg gcgacgacga acagtccgtc
     1021 acggaccagc gccttggcga tggcgcgacc gataccgcgg gatgctccag tgacgagagc
     1081 gacacgacgt agagaaggtg cttggctcat gaagaactcg ctacggttga gatggtttgg
     1141 tggaagcaag ctctgcgaca gcggcaagac cgcttggatc agcaaagcca gccagtgcga
     1201 cgtgagggac gatgcgcttg accagccctt gcaggacttt gcccggacct gcttcgacaa
     1261 tggcggtggc gccaagcgac gcgagctttt tgacgcagcc ttcccagcgc accgtaccgg
     1321 ccacttgacg caccagccga tcgcgcaccg cagacacatc gccttgcgga tagggctcgg
     1381 cgtcgatgtt ggcgaccacc gtgatccgcg gcgcccgcag cgtgatggag ccaagcgatt
     1441 cggccagttt cgccgccgcc ggctgcatca aagccgagtg gaacggtgcc gaaacgggaa
     1501 gcgctttgac cagcttgcag cctttggctt tggcctgcgc catcacccga tcgaccgctg
     1561 gtttgtggcc cgagatgacc acttgcccgg cgccattgtc attggccggc tgaacctttt
     1621 cctcttcggt cgatgcctcg acgcagagct gggcgacgtc atcgctggac agcccaacca
     1681 gcgcggccat cgcgccgagt ccctgaggca cggcctcttg catgaagcgg ccacgcaagt
     1741 gcacaagccg taccgcgtct gcaaagtcga tgctgtcggc tgcgaccagg gccgaatact
     1801 ccccaagaga gtgacccgca cacaaggctg gagccaaagc cagctcacgc cgcagcacct
     1861 cggcagcggc caccgagcag gtcaaaagcg ccggctgggt gttttcggtg cgaaccagct
     1921 cgctgtcagg cccctcgaaa cagagcgttg agagcgagta accgagcgca gcatccgcct
     1981 gatcgaacag cgaacgaacg tagggaaagg cgtcgaacag ggcgcggccc atgccgatgt
     2041 gctgagagcc ctgaccggga aaaacgaagg caatgttgtg catggttcct ctttagaagc
     2101 gcacgaccgt ggccccccaa gcgaggccag ccccgagcgc gcaaaagagc agccgatcgc
     2161 cacgcttcac cttgcccatc cgaaccgcct catcgagagc gatcggcacc gaggcactgg
     2221 aggtgttgcc atagcgatcg atgttgatga agaacttgtc catcggcagg tccagccgct
     2281 gcgcgaccgc ttcgacgata cgcaggttgg cttggtgcgc gatcacaagg tccacatcct
     2341 tgggcgtcag tccgtggtgc gcaagcgcag tcagacacga cgacgacagg ttgcgcacgg
     2401 cattggtgaa gatcaccttg ccgttcatgt ggatgaagtg cttgccgtcc gcaagcgtct
     2461 gcgccgacgt cggatacagc gagccgccgc cgggaatcat cagcgcttcg gcctgggtac
     2521 cgtctgcaaa caagtggacc gactgcacgc cgagtccagc ttcgtgctct tcttcggtgc
     2581 aaagccgcag aatggcagca ccagcgccgt cgccaaacag cacgcaggtg ttgcgatcct
     2641 gccagttgac gtagcccgac aggacttcga cgccgcacag caggatgttc cgatacaggc
     2701 cgctgcgcag aagaccttcg gccacggcca gtccatagat gaagccgcca cacgccgccg
     2761 aaaggtcaaa cgctgcgctg gtcggtgcag caccgacctt gcgctggacg tacaccgcgc
     2821 aggaaggcag cggcatgtcc gggctgatcg tcgaacaaat gaccgcgtcg atttcttttg
     2881 gagaaatccc cgcagcttcg caagcatggg tgcccgcgcg ggccgcaagg tcggaagcgg
     2941 cttcttcgcc ccgacgaaca gtgtgacgga cggatatgcc tgtccgctcg cgaatccaag
     3001 catccgacgt atcgaccatt ttgcttaagt cgtcgttggt aaggactctg tcggggacgg
     3061 cgcgacctgt gccggcaata cgaatccggc gcaggctggg agatggggtt acgggtttgc
     3121 ttttgctttg tgtatgtggg tcgtcactca ttcagcctcc ggttggccgg cttctacgga
     3181 tcgctactcg cggtgccatc accaaagcga tgggcacgta gggcagcttg ggtcgcttcg
     3241 atcagtccgg actggacgtg ttcttcggca acctgcactg cacgggcaat tgccctagca
     3301 ctggcgcgtc cgtggcagat gatcgccaca ccgtttacgc caagcaaggg ggcaccgcca
     3361 cgaccttcaa catcgacgac acgccgcaag gatttgagcg ctggcatcat cagcagcgca
     3421 ccaagctggg caagcagcga ccgctttacc tcgctgcgca gcagatcggc cagaaaacgc
     3481 ccagcgcctt cggcagtctt gagcacaacg ttgccggtaa atccatcggt cacgacgacg
     3541 tccaggccct catcacgcag cgcagtgttc gctgtgcggc gaaactggaa caagtcgcga
     3601 ccctcgacgt acccgacgta atcgaacgca gagcttgggt gcgccgtcaa aagagcgtgt
     3661 gtctcacgaa gaatcggcgt gcctttgctg gcttcttcgc cgtttgaaag cagaccgacc
     3721 ctgggacgca ggcgaggcga cgacgcacgg agcttggcaa actgcgcccc gaggatggca
     3781 aactgtgcca gggtctgggg ccgtacatcg acgtttgcgc ccgtatccaa aagcacgcac
     3841 tcaccaatcc gcggcggcga gccgaacaag cccggcacaa gcgttggcat ggtcacggcg
     3901 atccccggcc tgtccagccc agacatccgc ccgagcacaa acaagccgca cgcgagcatc
     3961 gcaccggagt ttcccgccga gacgaccgct gctgcttcgc cgctttttac cagctcgaag
     4021 ccaatgcgca tggaagactg acgcttcttg cgcacagcct gcccagggtg atcatccatc
     4081 gtgatcacct cggtgcagtg ccgaatggga ataccaaggt gcgcgccacc aagccgatct
     4141 agctcagcgc gaaggcgcga ttcgtcgccc aacaggatga cgcgcgcgcc tcgctcacgc
     4201 acagcagaga tggccccttc gacttcgggg ccaggcgcag tatcgccccc catggcgtcg
     4261 atcgcgatga caggtgccat cggaggcgcc atcgacaaac tatgccttct ctgccgccgc
     4321 ctcgttttcg gtcggctgcg ggatgaccac gcggccttcc ttggcaggtc cgtaccagcc
     4381 gcaggcctcg cagatgcggt ggggcatctt cgcggcctgg cagttcgggc aaaccgacga
     4441 cgcgggggtc gccatcttca tccactgggc acgacgcgaa tcacgtcggg cgctcgattg
     4501 tcttcttttc tgaacggcca tgttgctgtg atctccaggc tgatgtgaga gtgtgtcgtt
     4561 aaagttttac gtcgcgaagc gccgaaaatg gcgaaggctt gccgacaggc tggcaggagc
     4621 actcgccctg attcttgtca gcgccgcaga gaggacaaag tccgcgacag tcctctttac
     4681 agtgaacggt gatcggtacg gccaagatga tgaattcgcg cagtgaaggc gcaagatcca
     4741 ccgtttcgcc gtcgtgatgg gcgtagtcaa catcatcgac atcgtcgtcg gcttcgtcgc
     4801 ttgcttcccc atcgctactg cccaaggcag atgacggcgg aacaaacacc gtatgcagtc
     4861 gctgatcgac gaccacatgc gccggaccaa gacaaagctg gcatggcaga agaagttcgc
     4921 cggtcagctt gccatcgcac agcaccgtga cgttgtgttt ggtcacgtcc gccacaagcc
     4981 gcagcgacga ctgcgacaca tcaatgtcca tgtccgcaag cgcatccaaa atcatgtcag
     5041 ccggcagcac acagtcgatg tgccgttcct tgttttcaag gtcttttagc cggaagctaa
     5101 ggtctgtcat gcgctcttcg ccttggaagt cgcatcatag aaagtcgagt ctttctgtca
     5161 agatggccga ttcgtggcgc tttcagattg ggcacttggc cgctaagatg cgcgccatgc
     5221 caagctttgt ggtgacgcaa gaggatgacg acaagacgct cgcggcggtc gtgcgccaac
     5281 atctgggggt cgcgtggact gccgcgcgga cgatgtgcga gcgacacaag gtgcaaatcg
     5341 atggacagct tgcaccagat ccagtgcgac gggtgcggct tggccagacc atcacggtga
     5401 atcagacagc gccgcgacca atgccacagg ccgtggccgc cgcccagaag cgggtggtct
     5461 acgaagactc gcagctgatt gtcataaaca aaccagtggg gatgtccagc gtgccctacg
     5521 agcgcggcga aaccagcacc gcaatggaca tggtgcgcga cgcatggcgg gcaagcggac
     5581 gcaacgccac ggagcagccg ctgtacgttg tgcaccgcat cgacaaggac acgtcggggc
     5641 tgctgatgtt tgccaagacg cggaccgcag agcgtgatct ccagcagctt tttcggcacc
     5701 acgacatcag gcggcgctac atctgcgtcg tccacgggta ctttctaagc cagacgatcg
     5761 agacctatct catcaaagat cgcggcgatg gtctgcgcgg ctcgcttgcg ccctggaaag
     5821 ctgagctggg acacgcgggc cggctcgcgc tgacgcacgt tgaggtgctg gaacacattg
     5881 gaacgctggc gacggtctgc gctgtgcaaa tcgaaaccgg caagacccac cagatacgca
     5941 tccacctggc cgaagcggga catccgatca ttggcgagcg ggtctacatc cgcgacttca
     6001 cccgaaaagg tcaaacggcc ctgccctgcc cgcgtctttt gctgcacgcc gagacgctgg
     6061 ggtttgagca tccgaatacc ggcgacgacc tgatgttcca agaggaaccg ccgaccgact
     6121 ttgccgatgc agtggcacgc cttcgggcac aagcgcgcaa agagcgccag caagcagaag
     6181 cggcggctcg ctagccaggg cgcgctagtc cagcttatcg agatgcgcga aagctggccg
     6241 cagcttgtcg taagtcgctt gcagatgttc gctgatgact ttcacatcac cgacgacggg
     6301 catgaagttt gtgtcgccat tccagcgcgg cacaatatgg aagtgagcgt gctggtcgat
     6361 tccagcccca gcgacgcggc ccaggttcat ccccaggttt gcaccgtgcg cgcctagctc
     6421 ttttccgacg atctggttgg cctgtcgcag aagctcgcag gtggcttggt attcatcggc
     6481 ggggagcgcc atcaaatcgg cgccgtgtcg cctgggcacg accatgatgt gaccgctgcc
     6541 atacggaaag cgattcatga tggcaaacgc tgagcggttg cagcaaagga tcagatgttc
     6601 ggcaaaacgc gccggacctt ctgacggaaa ttggcaaaaa atgcactgct gcgctccctg
     6661 ctcaccgctt gcttgcagga tgtacgccat gcgccacggg gcccacaatc tgtccatgct
     6721 gtttcctttt tggcagccgt cagcgcaaaa gcagcgccgc ggtcacgccg cccaagataa
     6781 cagcgcagag cagcgcgaca atcaaccacg acgagcgctc ggtcttggcg ggcacaggag
     6841 cttggacgac ggcgggagcg ggtgaggtcg accgttccgc gaacagccgg gtttcattgg
     6901 cgcgcatctc ggtcgttggc atcgcctgca tggcgtccga aatcttcgcc gcagcttgcg
     6961 aaggcggcaa aatcacggtc cccggttcga ggttgcgcag tgcgtgaagg cgcgtggcca
     7021 cttcggccat cgtgggacgc tcggcggcct tttttcggag catggtgtcg accagcgcaa
     7081 tgatcggtcg cgccacgtcg ggggcttttt ccgaaagcgg cggcactggc tcgacaatgt
     7141 gcatggcaat gacatcgccc ggcgatcggc tgacaaatgg cggcgcgccc gatagcagct
     7201 gatacagcac tatgcccagc gcgtagacat ccgcccggtc ggtcacttcg acgccgccgc
     7261 ggcactgctc cggcgccatg taggtcggcg tccccagcaa aagcccagtg gccgtctgca
     7321 cccctggcag cgtctccccc gcgagcgccg caatcgcctt ggcaatcccg aagtcgagaa
     7381 tctttacgcg aaagccaaac ggcgcagacg catcgtcgat aagcagcagg ttgtccggct
     7441 tgaggtctcg gtgcacaatc ccggcggcgt gggcggcggc gagcgcttcg gcgatcgaca
     7501 aaccgatggc gacggcttct ttttgtccca gccggccctt gctgcgcaag cgctcccgca
     7561 gggactcgcc gaccaaaaac tccatcacaa agtagggacg cccccccggc aactgccccg
     7621 agtcgaagac tgtggtaagc cctgggtgat cgacacggcc catgaggcgc gcttccgcga
     7681 caaagcgctg caccgcagac aggttggtaa gcagcgattc gtggatggcc ttgaccgcga
     7741 cttgttggcc ggtctcgttg tgaacagcct cgaagaccac gcccatccca ccgcggccca
     7801 cctcgcgcag cacgcgatat tcaccgaccc gctgaccgat cagttgggtg ttgctatcgg
     7861 gactgcgtaa gggtgttgtc atcgacgaca gtcgaattcg gactatatca cgaggtgctg
     7921 gggcgatgac agcggaagta cgggagaggt gggcgagttt tgcgggcgac tagtttacgc
     7981 cgcaggccgt ccaagctgca gcgacggtcg ttgcttccgc actggaagct ccgtagagat
     8041 cggaagcagc actgatcgtc gcggtgcggg cgcccttgaa gttggtattc gaggtcatgt
     8101 agacggtgag cgcgcgatac caaatctttt cggctttgcc acgcccgatg ccagtgaccg
     8161 actgccccga ggtcttgttg gtgccgcctt cagccagcat gtggaagaac ttgttcgcca
     8221 gaccggagct gtagtgcaca tcgaggctgc tggtcatctg cgagtagtgg tcaatcgagt
     8281 agccatcggc gcgcgggtcg gccatgttgc gcagccagcg gtttccgtac agcgtgatct
     8341 tttcgccgat cagatagtcg ccggcgtcgg cggcgttgtt ggcgtagtac tcgaccatgg
     8401 tgccgaagat gtcactggtc gactcgttca gaccgcccga ctcacccgag tagaccaggg
     8461 ccgcagtgcg cgccgtgaca ccgtgcgaca tttcgtggcc ggccacgtcc agcgaaacca
     8521 gcgtgctgaa gctcttgccg tctccatcgc catacgtcat gcagaagcac gaatccgacc
     8581 agaaggcgtt gttgtacgcg cgaccgtagt gcacgcggtt atacgaaccc ttgccatcgt
     8641 tggcgatgcc gttgcgaccg tggacattct tgtaatagtc ccaagtcagc gcgacaccaa
     8701 cgtgggcgtc gacggcggcg ctcgccaggt cggcggcggt cccattgccc cacacgttgt
     8761 cgaggtcggt gaagatggtg ccgttgccgc tggttttttt gttcatgttg gtggtgtagc
     8821 caccgccacg atcaggatcg cgcagctcga aaccacccgt gatggagttg gcggtgagcg
     8881 acagcgtacc catgtagagg ctcttgcccg agcccactgc cgccgcctct ttgacttcgt
     8941 tgtggcggct gagatacgcg cccgtcagtg catcaacata ggtgtgctgg atgctggcaa
     9001 agtcatcctt gacttcgtcg ttgtagatca cgacgtcgta ggccagcttg ggcgagtccc
     9061 cgaatgcgtg gacgaccaag ctgactttgg ccagcaccgc cgtgcccgtg tagtggcgca
     9121 gcgcttccgc cgtcgccgaa atggcgctga tattcggctg aagcgccaca ctcaccggac
     9181 tcttcagcgt cgaactaaag ccgaggaagg tgctgccctt ctgatggagc accaagtcgc
     9241 cgccatggac cggcaggttg ctgtaggtcc ggttgtagtg ggtgtgggtc tggccaagct
     9301 ggtccgtcac cacagccgat tcctgaagcg tatcgctggt cccaagacca atcatcgcgc
     9361 cgttagactt tgccatcgcc aggctgttcg ggtcgccgcc aacatccgtc acaggagccg
     9421 tcggctgcac atccacttga ggaccaccgg gatcaagcac ctgatccatc ggagtctcgt
     9481 tgccgcaagc aacagccaac atcacagaag ccaggaatag attcttcgct t
//
LOCUS       DAORGN010000192         9450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3169_length_9450_cov_5.562108, whole genome shotgun sequence.
ACCESSION   DAORGN010000192 DAORGN010000000
VERSION     DAORGN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3169_length_9450_cov_5.562108"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKL17_17350"
     CDS             complement(<1..615)
                     /locus_tag="PKL17_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK46552.1"
                     /translation="MRVNALGTINVTRAALPLAHEGSAVVNVASIAGHMLPSLVRPKK
                     AFKLALSDPSAATDKLVASVQRGPAKMRPASAYSVSKAFVIWYSAHMAAAFGERGGRI
                     VSVSPGSFDTPMGRLEEKSGSAKLVEFAALKRFGRPEEVAEVLAFCAGPKPGYLTGVD
                     ILVDGGTERLETFGAVLLVELAQSAGALLAVGAAGIQQVVVGDLD"
     gene            614..2194
                     /locus_tag="PKL17_17355"
     CDS             614..2194
                     /locus_tag="PKL17_17355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNK46553.1"
                     /translation="MIWSADPISGLSPAVCTTDDTALAALRAALPFSPTRAQERAIAE
                     IRTDLARPHPMQRLLHGDVGSGKTLVAYAACELTMASGHQAAVMAPTEILAEQHYKTL
                     RVWAQATGRKLALLTATTPKTARKSTLAQLQAGRFAEVWRIAEATGQVLDRESILAML
                     AAGFLDMVVGTHALIADAVEFKDLALVVIDEQHRFGVGQRAALRRKGRQPHILVMTAT
                     PIPRTLALTLYGDLDVSQLDELPPGRTPPTTKVLPGRSGEKKALLAVRKAVSEGRQAY
                     WVCPLIEDSDKLELSSATARHAALCSSLPDLRVGLVHGRLGSDERDDVMDRFRRRELD
                     VLVATTVIEVGVDVPNASLMVIESAERFGLAQLHQLRGRVGRGAGASVCLLLHGSGLG
                     EESGTVLSAELLDDDDEEKKPRRRRKTADEKTETQASVPSALSRLEVVSATSDGFRLA
                     EADLRIRGPGEVLGTRQSGLPPLRFADLLRDIDLLTIARREAAALLSRDPDLCRPEHA
                     VTSRVLVERWSYCDLTLE"
     gene            complement(2238..2681)
                     /gene="rsfS"
                     /locus_tag="PKL17_17360"
     CDS             complement(2238..2681)
                     /gene="rsfS"
                     /locus_tag="PKL17_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNK46554.1"
                     /translation="MPARKNPSAANDKFRQDALACAQAAIDKKGIEPVLLDLTQAGSY
                     TDYLLITSAHADRQVRAVAEGIVSAMEERGVRLLGAEGLREGHWALLDFGDTVVHVFE
                     HPLREFYDLEGMWFDAPRLPLQIPAELRLDAQSAYRYDDEQPARP"
     gene            complement(2697..3425)
                     /locus_tag="PKL17_17365"
     CDS             complement(2697..3425)
                     /locus_tag="PKL17_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46555.1"
                     /translation="MESHDSPQTDSKHRRIVMVRSVFIAVANLVPLPGVSELLVDFSR
                     RGLIEHLAREHNVELEEGAVSALLEEAPEFQRLGILASLSKLGTLLRKQKRLRRLFTG
                     LQLLSGVEAGFRAFEMGSMFEHYLSTAHMGQKLSEDQARSVRKTMLDASRATEKELIG
                     DAISSLAMAVGQVALALPGYVWNRVASQGPQALSLPELSAVTQSATKLLSELRLQSYA
                     KKLTAHFDRKWTGPAVITVSQKPN"
     gene            complement(3450..4391)
                     /locus_tag="PKL17_17370"
     CDS             complement(3450..4391)
                     /locus_tag="PKL17_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46556.1"
                     /translation="MALAGACQIGCQNAPSSSRSTGPVSIGAQNDLVRGDRLLKVLSL
                     THKEVATRLGPHRIESQTRWTITPIPSQRAAQVPPVQPGFKLGSPAQPFSGDDAFETA
                     PVTMQETRFVEVDPSGRIHLGSENDHGGGIEAVTDPQYIFSKMRYQPFVRYRNEGSHS
                     ERLRQMAFDTGVALVETVGHRLKLGTPNSGNTHGRSTWQVALKMADTAQVPLRSQGSA
                     WRGAVNVESVEGLATVDREQGVPLAIELAVRFVAPRPGVPDERVQVEATHVMQVVALG
                     GEAVKFAIPSEFSDPPVRPRPQLDRQELLGGLVPSFR"
     gene            complement(4491..5222)
                     /locus_tag="PKL17_17375"
     CDS             complement(4491..5222)
                     /locus_tag="PKL17_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46557.1"
                     /translation="MRSFSMSQATLSLAALSVLLLTGCPKPPPPKLPPKEVPQIGQVG
                     TTAGERSDVVADAVQQGSNMARILFDCGVPHSQGADGKGVLSADLTKCEFDWAKKELT
                     ITDGSSAECPNFQLTITSFKGPGTYNTSSLGKLSFGTAKMRQSACNWDGNMCLDWNGT
                     SGPHPESSCTIEVNSDGGLQYGTSGSTLSGTFVCDGFQSSWKGCAGAPAKVACVISRA
                     SFSAAGCNVINKEEPKKKPGGKKAK"
     gene            5354..6940
                     /locus_tag="PKL17_17380"
     CDS             5354..6940
                     /locus_tag="PKL17_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNK46558.1"
                     /translation="MAERDFDYLIVGSGFGGSVAACRLSEKGYRVAVIEQGKRYREED
                     YAKSAWEMRKYLWMPALRCFGIFAMKVMKDLIVFHGAGVGGGSLVYANTHLEPPDSFY
                     RDPKWAGLADWKTELAPHYREAKRMLGSTLPPEFYETDEALRGVLSDMGTGHTFKKHT
                     VGVFFGPSGQKVPDPYFGGRGPDRTGCTTCGACMVGCRVGAKNTLDKNYLFFAEQRGA
                     KVFAETRVLAVRPIGSSDGSEGYEVETECSTSFRGNPRRKFTAQNVIFSAGVLGTVKL
                     LFHCRESGFLPNISTQLGRYVRTNSESISGAVAWGRDLSRGVAISSGGHTPDGTHVEI
                     YRYGQHADAMGLLGTVHTKGGKLPRQLYFLAALLRHPLMALRSSLWPFGWSKGMAGVL
                     AMQSVDNSMELHYKRSLLSPFSKSLASDWGDRPKPPTYLPEANEVTARLAKRMGAQPM
                     SVLPEVLLDTTTTAHILGGCSMGQGPSDGVIDRDCRVFGYRGLYVVDGSMIGANLGVN
                     PSLTITAMAERAMAQIPAKN"
     gene            complement(6953..8200)
                     /locus_tag="PKL17_17385"
     CDS             complement(6953..8200)
                     /locus_tag="PKL17_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNK46559.1"
                     /translation="MRITQSWLTVLFLSSVLTSACKDSTTPGDSPDMANSTDDLAMSL
                     PDLTDPGDLTPPPPDLIPSCGFGQAYNAGSCSCDTNHPVSCAGATYCCAANQACLSTP
                     GPRGETRCALPTTTFGRARHVMAYDKLRDRSVTRFGICDQGGTDVLCSDQWQLTPITW
                     DAPAVANPPTGRYDSTFIWDDNAKGLLLFGGLTVIGNVNTATNEMWLWDGTTWSKKTP
                     TTGPAARYAHAMAFDSLRKVAVLFGGEIGLTAQQDTWEWNNTQWLPRTTATVPQVRSD
                     HRMVYDTKAKRILMHGGFVSGAYSRETWAYDGSNWTQLPDDTSLSQRVKFGMAYDENR
                     QVTVVFGGEITGGVLVNDTWEYDGTKWTKKNPGTPPPKRVYPAMFYDAGRKQVLAFGG
                     DPINAGQYKDLWAFDGTNWTQLY"
     gene            8383..8973
                     /locus_tag="PKL17_17390"
     CDS             8383..8973
                     /locus_tag="PKL17_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46560.1"
                     /translation="MESLPLLFLGCLAGLSLPMLWRRRRPTREKPQASLAGDDLRRLG
                     PGAVLLYGGQDFVVIRAGRLAAAGPWERIVFLEHAQRPWVLLVAATGQTERLWLLALC
                     PDVLPQPHGSHPAASQKQLAGLHYQLGERYVTTWAALGQADESVRLDLYRGPGARRLL
                     LIRIGDAATGQGFLGDVVAEAALEVLPATSDGAPLR"
     gene            complement(8986..>9450)
                     /locus_tag="PKL17_17395"
     CDS             complement(8986..>9450)
                     /locus_tag="PKL17_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46561.1"
                     /translation="CDPNNYVTVLNTVADRITNRISANCLSGKPKTDPNGKPICIVGL
                     VDANTPAAMPDVSLPLCSASCCQAWAIAGDQSNPDPKVKAAKPIPSDPYIVSACTNDP
                     DCYCAVPNPTSNVACKDTAGNDTALSALWIKADPHQTPSGKVANFMCAVTVK"
BASE COUNT         1706 a         2770 c         3081 g         1893 t
ORIGIN      
        1 gtcgaggtcg cctactacga cctgctggat tccggctgcc cccactgcca gcagtgctcc
       61 cgcactctga gcaagctcaa caaggagtac ggcgccaaag gtctccagcc tctcggtgcc
      121 gccatcaacc aggatgtcga caccagtcag atagccaggc ttcggcccgg cacagaaggc
      181 cagcacttca gccacctcct ctggtcggcc gaagcgcttg agtgcggcaa actcgaccaa
      241 cttcgccgaa cctgacttct cctccaagcg ccccatcggg gtgtcgaaac tgccgggtga
      301 caccgacacg atccgacccc cgcgctcgcc gaaggcggcc gccatgtgtg cggagtacca
      361 gatcacaaac gccttgctca ccgagtacgc cgaggcgggt cgcatcttcg caggcccgcg
      421 ttgcacagag gcaaccaact tgtctgtcgc cgctgacggg tcgctcaagg cgagcttgaa
      481 cgccttcttc ggtcgcacca atgagggcag catgtgaccg gcgatcgagg ctacattgac
      541 caccgctgac ccctcatgag ccaacggcaa ggccgcccga gtgacgttga tggtgcctag
      601 cgcgttgact ctcatgatct ggtcggccga ccccatcagt ggactgagtc ctgcggtgtg
      661 cacgaccgac gacaccgcac ttgcggcatt gcgggcagca ctaccgtttt ccccgacgcg
      721 ggcgcaggaa cgggcgattg cggaaattcg taccgacctt gcgcggcctc atccgatgca
      781 gcggctgctg cacggcgacg ttgggtcagg aaaaacgctg gtcgcgtatg cggcgtgcga
      841 gctgacgatg gcaagcggtc atcaagctgc ggtgatggct ccgacggaaa ttttggccga
      901 acagcactac aagacgctgc gggtgtgggc ccaagcgacg ggacgcaagc tggcgttgct
      961 gactgcgaca acgccgaaaa ctgcgcgcaa atcgacgctg gcccagcttc aggccgggcg
     1021 gtttgccgaa gtgtggcgga ttgccgaagc gacgggacag gtgctcgatc gggaatcgat
     1081 tttggccatg ctcgcggcgg ggtttctcga catggtcgtc gggacgcacg cgctgattgc
     1141 cgatgccgtc gagttcaaag atttggcctt ggtggtcatc gacgaacagc atcgctttgg
     1201 ggtcggtcag cgcgccgcgc tccgtcgcaa aggccgtcag ccgcatatcc tggtgatgac
     1261 cgcgacgccg attccgcgca cgctggcgct gacgctgtat ggcgatctgg atgtcagtca
     1321 gctcgacgag ctaccaccgg gacggacgcc gcccacgacc aaggtgttgc cggggcgcag
     1381 cggagaaaaa aaagcgctgc tggcggtgcg caaagcagtg tctgaaggcc ggcaagcgta
     1441 ctgggtttgt ccgctcatcg aagacagcga caagctggag ctttcgagcg cgacggcgcg
     1501 acatgcggcg ctgtgttcgt ctttgccgga tctgcgcgtc ggtctggtgc acggtcggct
     1561 gggcagcgac gagcgcgatg atgtaatgga tcgttttcga cgacgagaac tggatgtgct
     1621 ggtggcgacg acggtgattg aagtcggcgt cgatgtgccc aatgcctcgc tgatggtgat
     1681 agagagtgcc gagcgcttcg gtttggccca gcttcatcag cttcggggtc gcgtcggtcg
     1741 tggcgccggg gccagcgtgt gtctgctttt gcacggcagc ggtctcggtg aagagtcggg
     1801 caccgtgctt tccgccgagc ttttggatga cgacgacgag gaaaagaagc cgcggcggcg
     1861 gcgcaagacg gctgatgaaa agacggaaac gcaagcaagt gttccgtctg cgctgtcgcg
     1921 cttggaagtg gtttctgcga cgagcgatgg ctttcgactg gcggaagcag acttgcggat
     1981 tcgtggacca ggagaggtgc ttggaacccg tcagtctggc ctgccgccgc ttcgatttgc
     2041 cgacctgttg cgtgacatcg acctgctgac gatcgctcga cgggaagccg cagcgctttt
     2101 gtcacgcgac ccggatctct gccgacccga gcatgccgtg accagtcgag ttctcgtcga
     2161 gcgctggtca tactgcgacc tgacgctcga atagcgcgaa gacggacccc aaaaatcgct
     2221 acgcggtctg gtccgcgcta cgggcgcgct ggctgttcgt cgtcgtaacg atacgccgac
     2281 tgggcatcca gacgcagctc cgctggaatt tgcagcggca gccgcggcgc atcaaaccac
     2341 attccctcga ggtcgtagaa ctcccgcagc ggatgctcga aaacatggac gacggtgtcg
     2401 ccgaaatcga gcagcgccca gtgtccttcg cgcagacctt ctgctccgag caggcgcaca
     2461 ccgcgctctt ccatcgctga gacaatgcct tcggcgaccg cgcgcacctg gcgatcggca
     2521 tgtgccgaag tgatcagcag atagtcggtg tagcttccag cttgggtcag gtcgaggagg
     2581 acgggctcaa tgcccttttt gtcgatggcc gcctgtgcac aagcgagcgc atcctgacgg
     2641 aacttgtcgt ttgcggctga ggggttcttg cgtgctggca agtctttctt ccttcgttag
     2701 ttgggttttt gactgacggt gatcaccgcc ggaccggtcc acttgcgatc aaagtgagcg
     2761 gtcagtttct ttgcgtagct ctggagtcgc agctccgata gcagcttggt tgcgctctgc
     2821 gtcactgccg acagttccgg tagggacagc gcttgcggac cttggctcgc gacgcgattc
     2881 cagacgtaac cgggcagggc gagcgcgact tgtccgacgg ccattgccag gcttgatatg
     2941 gcatcgccaa tcagctcttt ttccgtcgcc cgtgatgcat caagcatcgt cttgcgaacg
     3001 ctgcgcgcct gatcttcgga cagcttctgt cccatgtgcg ccgtcgacag atagtgctcg
     3061 aacatcgatc ccatctcgaa tgcgcggaag cctgcttcga cgccacttag aagctggagc
     3121 ccggtaaaca gccgacgcag gcgcttctgt ttgcgcaaaa gcgtgcccag cttgctaagc
     3181 gacgccaaaa tgcccaatcg ctgaaactcg ggcgcttcct cgagcagcgc tgacaccgcg
     3241 ccttcttcga gttcgacgtt gtgttcccgc gccaggtgtt cgataagacc gcgccgtgaa
     3301 aaatcgacga gcagttcgga cacaccaggc agcggcacaa ggtttgcgac agcgatgaaa
     3361 acactgcgca ccatcacgat gcggcggtgt ttgctgtccg tttgggggct atcgtgggac
     3421 tccatcgcgt gctctccttg gttcgccttt tagcggaacg acgggaccag ccccccgaga
     3481 agctcttgcc gatccagttg cggacggggc cgcacaggag ggtcggagaa ctcgctcgga
     3541 atggcaaatt tcaccgcctc tcctcccaag gctaccacct gcatgacgtg ggtggcttcg
     3601 acctggactc gctcgtcggg aacgcccggt cgcggcgcga caaaacggac cgccagttcg
     3661 atcgccagcg gcacgccttg ttcgcgatcg acggtggcca ggccttcgac ggattcgacg
     3721 ttgactgcgc cgcgccaggc cgacccttgt gatcgcagcg gcacttgcgc ggtatcggcc
     3781 attttcagcg ccacctgcca cgtcgagcgt ccgtgcgtgt tcccactgtt tggcgtcccg
     3841 agcttgagcc gatgtccgac ggtttcgaca agcgcgacgc cggtatcgaa cgccatctgg
     3901 cgaagcctct cgctgtggct gccttcgttg cgatagcgga caaacggctg gtagcgcatc
     3961 ttgctaaaga tgtactgcgg gtctgtcacc gcttcgatgc cgccgccgtg atcgttttcg
     4021 ctgccgaggt ggatgcgtcc gcttgggtcg acttcaacaa agcgcgtctc ttgcatcgtc
     4081 accggcgcgg tttcaaatgc gtcatcgccc gaaaacggct gcgccggact gccgagctta
     4141 aaccctggct gcaccggcgg aacttgcgcc gcgcgctgcg atggaatcgg cgtaatcgtc
     4201 cagcgcgtct gcgattcgat gcgatgtggc cccagccgcg tagcgacttc tttgtgggtc
     4261 agcgacagga ctttgagcag ccgatcgccg cgcaccagat cgttttgggc accaatcgaa
     4321 accggtcccg tcgatcggga agacgaaggg gcattttgac agccgatttg gcacgccccg
     4381 gcaagggcca tgcaaacaag ccagccctga aaccgcggtc ggttccgaag cctagagata
     4441 ggcagcgaca tgtactgcgc gcttagcgcc atttctcgcc gctgtcaagg ctacttggct
     4501 tttttgccgc ccggcttctt cttcggctct tctttgttga tgacgttgca gcctgccgcc
     4561 gaaaagctgg cgcgcgaaat cacgcaagcg actttggcgg gggctccggc gcagcctttc
     4621 cagctgctct ggaaaccgtc gcagacgaac gtgccggaca gcgtcgaacc actggtgccg
     4681 tactgaagtc cgccgtcgga attgacttcg atcgtgcagc tcgattcggg gtgcgggccg
     4741 cttgtgccgt tccagtcgag gcacatgttg ccgtcccagt tgcacgccga ctgccgcatc
     4801 ttcgcggtgc caaacgacag cttgccgagc gacgacgtgt tgtacgttcc tggtccctta
     4861 aagctggtga tggtgagctg gaagttcggg cactcggcgc tgctgccgtc ggtgatggtc
     4921 agctcttttt tggcccaatc gaactcgcac ttggtcaggt cggcgctcag gacgcctttg
     4981 ccgtcggcgc cttggctgtg tggaacccca caatcgaaca gaatgcgggc catgttgctg
     5041 ccttgctgga ctgcgtcagc gacgacatca ctgcgttcac cagcggtggt gccgacttgg
     5101 ccgatctgcg gcacttcttt gggcggcagc ttcggcggtg gcggcttggg acagccggtc
     5161 agcaacagaa cggacagagc agcgagggac agcgtggctt gcgacatgga aaaagagcgc
     5221 atgcgacgat tttacacgag cgagaaaaga tgagccagaa tcctaagatg aggattttgg
     5281 tttccaatca ttcaaaaaaa gcgtgtcgta aaaaagacga gtgaagcgct ttggtgcgca
     5341 ggagggaact cccatggcag agcgagactt cgactacctg atcgtcggtt ccggcttcgg
     5401 tggcagcgtc gccgcgtgtc gtctgagcga aaaaggctac cgcgtcgccg tcatcgaaca
     5461 aggcaagcgc tatcgcgaag aggactacgc caaatccgcc tgggaaatgc gcaaatacct
     5521 gtggatgccc gcactgcgct gcttcggcat cttcgcgatg aaagtcatga aggatctgat
     5581 cgtgtttcac ggcgctggcg tcgggggcgg atcgctcgtg tatgccaaca cgcatctgga
     5641 accgccggac agcttctatc gcgatcccaa atgggccggg cttgccgact ggaaaaccga
     5701 gctagccccc cattaccgcg aggccaaacg gatgctcggg tccaccctgc cgcccgagtt
     5761 ttacgaaacc gatgaagcgc tgcgcggcgt gctgtccgac atgggaactg ggcacacgtt
     5821 caaaaagcac accgtcggtg tgttctttgg tccatcgggt caaaaagttc ccgatcctta
     5881 ctttggcggg cgcggccccg atcgaaccgg ctgtaccacc tgcggcgctt gcatggtcgg
     5941 ctgtcgcgtc ggtgcgaaaa acacgctcga caaaaattac ctgtttttcg ctgagcagcg
     6001 cggggccaag gtgtttgccg aaacgcgggt cttggcggtt cgtcccatcg ggtcgagcga
     6061 cggcagtgaa ggatacgaag tggagaccga gtgctcgacg agcttccgtg gcaatccacg
     6121 acgcaagttc accgcgcaga acgtgatctt ttcggcgggc gtgctcggga cggtaaagct
     6181 cctgttccac tgtcgcgagt cggggttttt gccaaacatc tcgacgcagc ttgggcgcta
     6241 tgtgcggaca aactccgaat cgataagcgg cgcggtggca tggggacgcg acctgtcgcg
     6301 cggcgtggcg atcagctcgg gcgggcatac acccgacgga acccacgtcg aaatttatcg
     6361 ctacggccag catgctgacg cgatgggtct gctcggcacg gtgcatacga aaggcggcaa
     6421 gctgcctcgg cagctgtatt ttttggcggc gcttttgcgg catccgctca tggccctgcg
     6481 cagctcgctg tggccgtttg gttggtccaa aggaatggcg ggggttttgg cgatgcagtc
     6541 cgtcgacaac tcgatggaac tgcactacaa acggagcctg ctttcgccgt tttcaaagtc
     6601 gctggcgagt gactggggcg ataggcccaa gccgccgacg tatctgccgg aagcgaacga
     6661 ggtgacggcg cggctggcga agcgaatggg ggcgcagccg atgtcggtgc tgccggaagt
     6721 gctgctggac acgacgacga cggcgcatat cctcggcggc tgttcgatgg ggcaggggcc
     6781 ttccgacggc gtgatcgatc gtgactgccg cgtatttggc taccgaggtt tgtatgtcgt
     6841 cgacggatcg atgattggcg ccaacttggg ggtcaatcca tcgctgacga tcaccgcgat
     6901 ggcggagcgc gcgatggcgc agattccggc caaaaattag ccggcgtcac cgttagtaaa
     6961 gctgggtcca gttggtgccg tcaaaagccc acagatcctt gtactggccg gcgtttatcg
     7021 ggtctccgcc aaacgcgagc acctgtttgc ggcccgcgtc gtagaacatc gctggataga
     7081 cgcgcttggg cggcggcgtg ccgggatttt tcttggtcca cttggtgccg tcatattccc
     7141 aggtgtcgtt gacgaggaca ccgccggtga tttctccgcc aaacaccacc gtcacttggc
     7201 gattttcgtc gtaggccatg ccaaacttga cgcgctgtga cagcgaggtg tcgtcgggga
     7261 gctgggtcca gttgctgccg tcataggccc aggtttcgcg gctgtaggcg ccagagacga
     7321 agccaccgtg catcaggatg cgcttcgctt tggtgtcata gaccatgcga tggtcggatc
     7381 ggacctgggg caccgtcgct gtcgtgcgtg gcagccactg ggtgttgttc cattcccagg
     7441 tgtcttgctg cgccgtcagg ccgatttcgc cgccgaacag caccgcaact ttgcgcagcg
     7501 agtcgaatgc catcgcatgg gcgtagcggg cggcgggacc ggtcgtgggc gttttcttgc
     7561 tccacgtcgt gccgtcccac aaccacatct cgtttgtggc ggtgttgacg ttgccgatga
     7621 cggtgagccc gccaaacagc aagagtccct tggcgttgtc atcccaaata aacgtcgaat
     7681 cgtagcgtcc cgtcggaggg ttggcgacag ccggcgcgtc ccaggtgatc ggcgtaagct
     7741 gccactgatc gctgcacagc acgtcggtgc cgccctgatc gcagatgcca aaccgcgtca
     7801 ccgatcgatc gcgcagcttg tcgtaagcca tcacatgacg agcgcgtccg aacgtcgtcg
     7861 tgggcagcgc gcagcgtgtt tcgccgcgtg gcccaggggt tgaaagacac gcttgattgg
     7921 ctgcacagca gtacgtcgcg ccggcgcatg aaaccgggtg atttgtgtca caactgcaac
     7981 ttcctgcgtt atatgcctga ccaaatccac acgaagggat cagatcgggg ggcgggggcg
     8041 ttaggtctcc cggatcggtc aggtcgggca gcgacatcgc caggtcatcg gtcgagttcg
     8101 ccatgtcggg cgaatcgcca ggcgtcgtcg aatctttgca tgcgctggtc agcacagagc
     8161 ttaggaaaag gacagtaagc caggactgag tgattctcat cggacgggct cctattgcga
     8221 gaggtccgcc acgattatgg ccaagccgtg catgaacggc caatgtttgg ttatttagaa
     8281 ttcaaaattt cataagaaag aaagctcagc aggagcgctg cgatttggat ggcttggcgc
     8341 gtgttttgga cacgggcgaa ccggcacgca caatgacaag acatggagtc gctgccgctt
     8401 ttgtttctgg gttgtctggc tggactgtcg ctgccgatgt tgtggcgccg tcgccgtccc
     8461 acgagagaaa agccgcaggc atcgctcgcg ggtgacgatc tgcgacggct gggtcccggc
     8521 gcggtgcttt tgtacggcgg acaagacttc gtggtgatac gggcggggcg tctggctgcg
     8581 gcgggtccgt gggagcggat cgtgtttttg gaacacgcgc agcgtccgtg ggtgctgctg
     8641 gtggctgcca cgggacagac ggaacggctg tggctgcttg cgctgtgccc ggatgtcttg
     8701 ccgcagccac atgggtcaca tccagcggct tcgcaaaagc agcttgcggg gctgcactat
     8761 cagcttggcg agcggtatgt gacgacgtgg gcggctttgg gccaagcgga tgaatcggtg
     8821 cggctcgact tgtatcgggg gccgggcgcg cggcggctct tgctgatccg aattggggat
     8881 gcagcgacgg gccagggctt tttgggggac gtggtggcgg aagcggcgct agaggtgctc
     8941 cccgcaacga gcgacggagc acctctccgg tagcggcgag acggcttact tcacggtgac
     9001 ggcgcacatg aagttggcga ccttgcccga cggcgtctgg tgcggatcgg ccttgatcca
     9061 cagagccgac agcgcggtgt cgttgccagc agtgtccttg caggcgacgt tcgaggtcgg
     9121 gttcggcacg gcgcagtagc agtccggatc gttggtgcag gcgctgacga tgtacggatc
     9181 gctcgggatc ggcttggccg ccttgacctt cgggtcgggg ttgctctgat cgccggcaat
     9241 cgcccaggcc tggcagcagc tcgccgagca gagcggcagg ctgacgtcgg gcatggccgc
     9301 cggggtgttg gcgtcgacca gaccgacgat gcagattggc ttgccgttcg gatcggtctt
     9361 cggcttgccg ctgaggcagt tggccgagat gcggttggtg atgcggtccg cgacggtgtt
     9421 cagaaccgtg acgtagttgt tcggatcgca
//
LOCUS       DAORGN010000193         9396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3196_length_9396_cov_6.024301, whole genome shotgun sequence.
ACCESSION   DAORGN010000193 DAORGN010000000
VERSION     DAORGN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3196_length_9396_cov_6.024301"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1092
                     /locus_tag="PKL17_17400"
     CDS             241..1092
                     /locus_tag="PKL17_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNK46562.1"
                     /translation="MSKQLSGVGIGLRPRFLDRDGLLSTKRQTDFLEIVPENWLLRGG
                     KRKSQLASCRDRWPMVPHSVSLNIGGLDPLDGELLDGIAALCDDFASPFFSDHLVYSS
                     VAGQPLHDLLPLPFTEEVVEHVARRVKQAESRVGRPLVLENATFYAHMPGCTMSEAAF
                     LHAVCEAADCGLLLDVNNVYVNSQNHGFDPYRFIDKMPLERVRQLHLAGHTLVGDTII
                     DTHIGPIISPVWALYRYTLQRAGRLIPTLIEWDQEIPHLDDVLDEADRARELATAALS
                     GGSDEAR"
     gene            1079..1867
                     /locus_tag="PKL17_17405"
     CDS             1079..1867
                     /locus_tag="PKL17_17405"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNK46563.1"
                     /translation="MKLADFFAQMEPFLLGKASHAATVEQLYGASPPQPDADRLAIYG
                     RFCDVHRHEVLDGIFPYLRTIVVRLHGESGWDTLVRGYFDAHPMHHFELNHNAVALPS
                     FLGSLDASSLGIALPDYAAALADFEWWEWQTLSVEETADDLLPDGGPLRLHGSVELRP
                     YPYDLLSWIEAADDLSCAEPPSPEKIDSLVLFWRDRTQSLRRDRVSSVEIQIIKAVYE
                     EHRLDAALAEAMSIPIEQLAETALDLYQAGIVIGDAKLLPSTSS"
     gene            1948..3486
                     /locus_tag="PKL17_17410"
     CDS             1948..3486
                     /locus_tag="PKL17_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase"
                     /protein_id="HNK46564.1"
                     /translation="MALSLASLRRQFPLTSAAFSQIPDGGQAALLHIQQLDARYLAPR
                     TLLPPSSTPSSSLGEFDVVLAGGGLSLIYAALLAQRGLRVAVFDRRQIALGHREWNIS
                     RRELTPLWQSGLFQKNEVDRLVLMQYRSGICRFADGPDYAVSGVLDCVVDAEALLSSL
                     HQLCILAGVTLLPQRELQSYSVDSSGVTVCIAPTSSPLHQESLRAKLLIDGLGAMSPH
                     ARFDLCCPTVGGVMDDLEIGDARQQMNPALGEILVTTEGIEDGRQHIWEGFPTVSAQG
                     DAVSTRRMTVYLFYYLRTELLPWHGPLPLLNLYERFFATLPRYKRGPLALVRPTYGYI
                     PAYTRLHKMPKAPRDRVLLVGDAAGRHSPLTFCGFGSMVRSFLPIAEGVIRLVGENRL
                     DASSLSRLWTEPPALQVMGGLTLMMAPPLTADGSDDPGTVNALLNAAFSTLHAQGEET
                     YRRFLQDDVDGPTFVRFMLGAAQKHPDVYRKVFRYLSLSEVSSWLFRLWQLYRAVPSP
                     QRES"
     gene            3593..7441
                     /locus_tag="PKL17_17415"
     CDS             3593..7441
                     /locus_tag="PKL17_17415"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46565.1"
                     /translation="MSLVDPPSDAIPSRAELRRLLDVLLPGDNDLNGFVYDHFPSVIQ
                     RFQPGLSRNAKLDLLLTFAEFSDLHRRLRERGLHQRKRRTTEIPDFGSERSRHPDLFG
                     REDLLSTLLAACQQDGWVVVSGAPGVGKTALLSHLVNRLEQQLGSPVPHHFLRRSVSD
                     SARPGVVLRSLASQVEARFPRQADPDAPPELRLIELLHRVSQGPLAKGGRLILVVDGL
                     DEAESEGMSNPLPRFLPAELPQSVTLVCSVRPRYAHFGWLTDHASRHLVGQFDLDASP
                     WNASGFDAVMRYFAEHGRRLSLDPDYATRVAVSADGNLLYAAKLREYIERLCSDGQPL
                     PRAELLPSGLRQFLIRLWDGLPQDVRAGLALLCAARQALPLPLLGELLGWKAGSDTES
                     QFVRLARPLLFLEPAPASTSVPGDCVRFDHAALRDWVEEQIGPTSLHQNRRQVAAALC
                     AWPPLEDSLYGFRRLYALRHAVTQHIETGAIAQAEQIAGNVDYLIAKCQEFGSASLAE
                     DLEHAAASCGQREPARTFAALAQALRVGAHWLRQDPGALPGLLYNLLRCANWSSAAIE
                     RVLHYPPGRLRFRLRFPLQRRDASVHTFAGHWDSVVAALPTPDGKRLVSVSLDRTLRL
                     WDLNSGAPLMHFYGCVGNAGSFVVAQNGQSLIYATEDHSLVMYDLGSGTLLRRFRGHS
                     AKISVCLLTPDGQLVIAAASDNSVRVWRVQTGEQLAMLSGHQSPPSSCACTKDSRYLI
                     TGSWDHTLRVWDLKTGRNVRTLQGHTGAVSGFALSSDGKYVVSASWDHTLRLWDVNTG
                     AKVHVLVGHTAPVNGCTITPDGRFAISASDDSTLKIWDLHGGRELRTLFGHSAAVKGC
                     VLSHDGKSVLSVSEDATLRQWDVSTGSLLRTFAGHLATVTCCAVAPDGRQLISASEDR
                     TLKLWDLSAEPDGNREGGHVDAITGLWMSSDGQLLVSGSEDRTVKVWDLLAGTVMSTI
                     FGHEDAVTACTGSLDGKTLVTVSADRTIAVWDLQTGSEKLRFSTTSTPSGEHAKSGSA
                     ALAFPSDDHMIELWGMPMDVQRSGEISLRGRVRGCAMTPDGKYLVTGASDKLLRLWDL
                     RTGAELLRFVGHTGAVNACSILPGGVHMVTASADKTLVLWDLRTGGEVLRFLGHAQSV
                     SACAVSTDGRRLLSGGNDKSLRLWDLQTGAELLRLLGHIAPVTGCAFSSDGKRAVSCS
                     LDYTLKLWELHSGMCLETVYGSAPFLSVVTQGDWLCAGDQAGNVWMLRDMTPQSTQSG
                     EPNSRASIVESIRKLLSLGR"
     gene            complement(7540..7920)
                     /locus_tag="PKL17_17420"
     CDS             complement(7540..7920)
                     /locus_tag="PKL17_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46566.1"
                     /translation="MRRNSSFRRLLAASLLALGVASCGGRPDNMVIVAVSGLLPRITE
                     LYVTMQLDDIAAKNSRPVPESGSDGFVVYQQMERFAVEVPDNTQRITIDIKGFDTARS
                     VVRQGTGSLSLSQSHDLSIELVAP"
     gene            complement(7937..8809)
                     /locus_tag="PKL17_17425"
     CDS             complement(7937..8809)
                     /locus_tag="PKL17_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46567.1"
                     /translation="MISALFAGIYGHIPRTLLLALLVGSLTVRSASAKGDSAEQHYSR
                     GLAFYESKDYPSAIREFQAAYKIRQLPRILLNIGQVYRKLGMASTALRFYEHYLRVDP
                     KPKPEIKAEVDRYIAMTRAMLDPPSFEAVDAKSAKAAAAAAVAAAPSNVTPGSVATVD
                     PFDIDSTSKPSDGKTAPAAKTAPEKTAATVTQTPATATGGNTAQTAVPTPGTSTAAPE
                     VKSILPTTAPSDANQALTQTPPVDKPATKPFYKQPLFWGVVGGVAAAVTITGIAVGVS
                     QSNSIPATVLYPTK"
     gene            8999..>9396
                     /locus_tag="PKL17_17430"
     CDS             8999..>9396
                     /locus_tag="PKL17_17430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46568.1"
                     /translation="MEGQRIGNYRVVRKIGQGGMGSVWEAKHEQLGRKAAIKLLRREL
                     TADPQVEVRFFNEAKAASRVDHPSIVKIDEFGHLPDGTAYIIMEYLSGEPLSVRLRNL
                     GGKLQLPDIFRISRQIASALAATHAKGIIHR"
BASE COUNT         1624 a         2677 c         3097 g         1998 t
ORIGIN      
        1 ggcggagcca agagctgcgg cggcgacaag ggcggagcca agagctgcgg cggcgacaag
       61 ggcggagcca agagctgcgg cggcgccaaa ggcggcaagt aggcagcagc gcggacgcac
      121 ggtctgttcc gactgtgcgc ttgggcagta agcaaaggtc acggcgagcg cgcgtggcct
      181 tttttttgcg cgcagctcgc ggattccaca gatactgccc aaacgggact gggaacactg
      241 gtgagcaaac agctatctgg cgtcggaatt ggccttcgtc cgcggttttt ggatcgtgat
      301 ggtctgctct cgacgaaacg gcagacggac tttctggaaa tcgtgccgga aaactggctg
      361 ctgcgcggcg gcaagcgcaa atcgcagctg gcgtcgtgtc gggatcgctg gccgatggtt
      421 ccacacagcg tgtctttgaa catcggtggg ctcgatccgc tggatggtga gctgctcgac
      481 ggaattgctg cgctttgcga tgactttgcg tcgccgtttt tttccgatca cttggtttat
      541 tcttccgtcg ctgggcagcc gctccacgac ttgttgccgc tgccttttac cgaagaagtc
      601 gtcgaacacg tcgctcgccg ggtgaaacag gccgaatcgc gcgtgggcag gccgctggtg
      661 ctcgaaaatg cgacgtttta tgcccatatg ccaggctgca cgatgagcga agcggcgttc
      721 ctacacgcag tctgtgaagc ggcggactgt gggctgctgc tcgacgtgaa caacgtgtat
      781 gtgaacagcc aaaatcatgg ctttgatccg tatcggttca tcgacaagat gccgcttgag
      841 cgggttcgtc agctgcattt ggccggccat acgctcgtcg gagacaccat cattgatacc
      901 cacatcgggc cgatcatctc gccggtctgg gcactgtatc gctatacctt gcagcgggcc
      961 ggacgactga ttccgacgct gattgagtgg gatcaggaaa ttccgcacct tgacgacgtg
     1021 ctggatgaag ccgatcgggc acgggaattg gcgacggcag cgctatcagg aggcagcgat
     1081 gaagctcgct gatttttttg cccagatgga accctttctg ctgggcaaag cttcgcatgc
     1141 ggcgacggtt gagcagctgt acggcgcttc accgccgcag ccggatgccg atcggttggc
     1201 gatatatggt cgcttttgtg atgtgcatcg ccacgaagtg ctcgacggga tttttcctta
     1261 tctgcggacg attgtggtgc ggctgcacgg ggagtctggc tgggacacgc tcgtgcgtgg
     1321 ctatttcgac gcccatccga tgcaccactt tgagctaaac cacaacgccg tcgcgcttcc
     1381 gtcgtttctg ggttctctcg atgcatcgtc gctggggatt gcgctgcccg attatgcggc
     1441 ggcgctggcg gacttcgagt ggtgggagtg gcaaacgctg tcagtcgagg aaaccgcgga
     1501 cgatctcttg cccgacgggg gaccgctgcg cctgcatggc tctgtcgagc tgcgtccgta
     1561 tccctacgat ctgctgtcgt ggatcgaggc cgcagacgat ttgtcctgcg ccgaaccgcc
     1621 gtcgccggaa aaaatcgata gtttggtgtt gttttggcgt gatcgcaccc agtcgctccg
     1681 tcgggaccgg gtcagttccg tcgaaattca gatcatcaaa gcggtgtacg aagaacatcg
     1741 actcgacgcg gcgctggctg aagcgatgtc gatcccgata gaacagctcg cggaaactgc
     1801 gctggatttg taccaggccg gcatcgtgat aggggacgcc aagctgttgc caagcacttc
     1861 ttcttagcac gctctcttcg tcgagaaacc cagcgcagtg gcggtgcggg cggtgcaaac
     1921 gcgcgggctc ggtctatagt gcacgccgtg gctctttctt tggcctcttt gcgtcggcag
     1981 tttccgttaa cctctgccgc attttcccag atccccgacg ggggacaggc ggcgcttttg
     2041 cacattcagc agctcgatgc gcgctatctg gccccgcgga cgctgctgcc accgtcatcg
     2101 acaccgtcgt cttctctcgg tgagttcgac gttgtactgg ctggcggggg tctgtcgctg
     2161 atctatgccg cactgctcgc gcagaggggg ctgcgagtgg cggtgtttga tcgccggcag
     2221 attgccttgg gacatcggga gtggaacatt tctcgacgag aattgacacc gctgtggcag
     2281 tcggggctgt ttcaaaaaaa cgaagtcgat cggttggtgc tgatgcagta ccgcagcggg
     2341 atttgtcgct ttgccgacgg gccggactat gcggtgtctg gcgtgctcga ctgtgttgtt
     2401 gatgccgaag cgctgctttc gtcgctgcat cagctctgca ttttggctgg ggtgacgcta
     2461 ttgccacagc gtgagctgca aagttattcc gtcgacagct cgggcgtcac tgtttgcatc
     2521 gcaccaactt cgtcgccgct ccatcaggaa tcgctgcgcg ccaaactgct tatcgacggg
     2581 cttggggcaa tgagtccgca tgcccgattc gatctgtgtt gtccgacggt gggcggcgtc
     2641 atggatgatc tggaaattgg cgatgcacgg cagcagatga acccggcgct gggggaaatt
     2701 cttgtcacca ccgaaggcat cgaagacgga cggcagcaca tctgggaagg ctttccgacg
     2761 gtctcggcgc agggcgacgc agtatcgacg agacggatga cggtgtatct gttttactac
     2821 ctgcgcaccg agcttttgcc gtggcacgga ccgctgccgc tgctgaacct gtacgaacga
     2881 ttttttgcga cgctgccgcg ctacaagcgg ggaccgcttg cgctggtgcg tccgacctac
     2941 ggctacattc cggcgtacac gcggctgcac aagatgccaa aagcgccgcg cgatcgggtg
     3001 ctcctggtcg gtgatgccgc gggacgacac tcgccgctga cgttttgtgg atttggttcg
     3061 atggtgcgtt catttttgcc aattgccgag ggtgtgatcc ggctggttgg cgaaaatcgg
     3121 ctcgatgcga gctcgctgtc taggctgtgg accgaaccgc cagcgctcca agtgatgggg
     3181 ggactgacgc tgatgatggc gccgccgctg accgccgatg ggtcagacga tccgggcacc
     3241 gtcaatgcgc tgctcaatgc cgcattttcg acgctgcatg cgcagggtga ggaaacctat
     3301 cgacgctttt tacaggacga cgtcgatggg ccgacgtttg tgcgcttcat gctcggggcg
     3361 gcgcaaaaac atcccgatgt ctaccgcaag gtcttccgct atctgtcgct gtcggaggta
     3421 agctcgtggc tgtttcgctt gtggcagctg tatcgtgccg tgccgtcgcc gcagcgtgag
     3481 tcgtagcaat ttgggctgcc gccgcgtgga ctctgcaaat aatttctgag aattgaatta
     3541 caatgttacc ggcctggtgg aaggacattc cgcttcgggc tgagcacacg gaatgagtct
     3601 ggtcgacccg ccttcagatg caatcccctc gcgcgctgag ctgcgccgtc tgctcgacgt
     3661 gctgctgccc ggcgacaacg acctaaacgg ctttgtctat gaccattttc cgtcggtcat
     3721 tcagcgcttt cagcccggtc tgtcgcgcaa tgccaagctg gatctgctgc tgaccttcgc
     3781 cgagttttcc gaccttcatc gtcggttgcg agaacgcggt ctgcaccagc gcaagcgccg
     3841 aacgacggaa attcctgact ttggatcgga gcgatcgcgt catccggatc ttttcggacg
     3901 cgaagacttg ctttcgacgc tgctggccgc ctgtcagcaa gatggttggg tcgtggtaag
     3961 cggcgcgccg ggcgtcggca agacggcgct gctttcgcac ttggtcaatc ggcttgagca
     4021 gcagcttgga tcgccggtgc cgcatcactt tttgcgtcgc tcggtttcgg attcggcccg
     4081 gcctggggtg gtgttgcgct cgctcgcatc gcaggtcgaa gcccgcttcc cgcgtcaggc
     4141 cgatccagat gcgccgccgg aactgcgtct gattgaactt ttgcatcggg tttcgcaagg
     4201 gccgctcgcc aaggggggac ggctgatctt ggtcgtcgac gggcttgatg aggctgagtc
     4261 ggaagggatg tcaaatccgc tgccgcgctt tctgcccgcc gagcttccgc agtcggtgac
     4321 actcgtctgc tcggtgcgtc cgcgctatgc ccactttggc tggttgaccg atcatgcgtc
     4381 gcgccatctc gtcggacagt tcgatctgga cgcctcgccg tggaatgcgt cgggtttcga
     4441 cgcggtgatg cgatatttcg cagagcatgg caggcggctt tcgcttgacc ccgattatgc
     4501 gacgcgcgtg gcggtcagtg cagacggcaa tcttctttat gccgccaagc tgcgagagta
     4561 catcgaaagg ctgtgcagcg acgggcaacc tctgccccgt gccgagctgt tgccaagcgg
     4621 cctgcggcaa tttttgattc ggctgtggga cggtctgccg caagatgtcc gggcgggtct
     4681 ggcgcttttg tgtgcggcac ggcaagcgct gccgctgccc ttgctcggcg agcttctggg
     4741 ctggaaggcc ggcagcgaca ccgagtccca gtttgtgcgg cttgcgcgcc cgctgctgtt
     4801 tttggagcca gcgcccgcgt cgacatccgt gccgggcgac tgcgttcgct tcgatcatgc
     4861 ggcgctgcgt gactgggtcg aagaacagat cgggccgacg tcacttcatc aaaaccgtcg
     4921 acaggtcgct gctgcgctgt gtgcctggcc gccgctggaa gacagtctgt atggtttccg
     4981 gcggctgtac gcgctgcggc acgcggtgac ccagcacatc gaaaccggtg ccatcgcgca
     5041 ggccgagcag attgccggca atgtcgacta cttgatcgcc aagtgccaag agtttggcag
     5101 tgcgtcgctc gccgaagacc tcgagcatgc ggcggccagt tgtgggcagc gcgagccggc
     5161 gcggacgttt gccgctttgg cacaagcgct gcgtgttggg gcacactggc ttcgtcaaga
     5221 tccgggcgcc ctcccgggac ttttgtacaa ccttttgcgc tgcgccaact ggtcgagcgc
     5281 ggcgatcgag cgggtgctgc actatccgcc gggccggctg cgtttccgac tgcgatttcc
     5341 gcttcagcgc cgagacgcat cggtccatac ctttgctggc cactgggatt cggtggtggc
     5401 ggcgctaccg acgcccgacg gcaagcgatt ggtatcggtg tcgctcgatc ggacgctgcg
     5461 actttgggat ctcaacagcg gtgcgccgct gatgcacttt tacggctgtg tcggcaacgc
     5521 gggttcgttt gtggttgccc aaaacggcca gagcctgatt tatgcgacgg aagaccactc
     5581 gctggtgatg tacgacctgg ggagtgggac gcttttgcga cgcttccgtg gccacagcgc
     5641 caaaatcagc gtgtgcctgc ttacgcctga cggacagctc gtgattgcgg cggcgtcaga
     5701 caacagcgtt cgcgtctggc gggtgcagac gggtgaacag cttgcgatgc tgagcggaca
     5761 ccagtctccg ccgagcagct gcgcgtgcac caaagacagc cgctacctta tcaccgggtc
     5821 gtgggaccac acgctgcgcg tctgggatct gaaaacgggg cgcaatgtcc ggacgctgca
     5881 aggccacact ggcgcggtca gtggttttgc gctgtccagc gatggcaaat atgtcgtttc
     5941 agcgtcgtgg gatcatacgc tgcggctgtg ggacgtaaac accggcgcca aagtgcatgt
     6001 tcttgtcgga cataccgcgc cggtcaatgg ctgcacgatc acacccgacg gtcgctttgc
     6061 catttctgcg tccgacgata gcacgctcaa aatctgggat ctgcacggtg ggcgcgagct
     6121 gcgcacgctg tttggtcact cggcggcggt caaaggctgt gtgctgtctc acgatggcaa
     6181 gagtgtgctg tcggtgtccg aagatgccac gctgcgacag tgggatgttt cgacgggaag
     6241 cctgctgcgg acgtttgctg gtcacttggc gacggtgacg tgttgtgcgg tcgcgcccga
     6301 cggacggcag cttatttccg cgtcggaaga tcgaacgctc aagctgtggg atttgtcggc
     6361 ggaaccggac ggcaatcgcg aaggcggaca cgtcgatgcg atcaccggtc tgtggatgtc
     6421 ttctgacgga cagctcttgg tgagcggcag tgaagatcgg acggtcaagg tctgggatct
     6481 gctcgctggg acggtgatga gcacgatctt tggccacgaa gacgcggtga cggcttgcac
     6541 cggctcgctc gacgggaaaa cactggttac ggtgtctgcc gatcgcacaa ttgcagtgtg
     6601 ggacttgcag acgggctctg aaaaactgcg cttttcgacg acctcgaccc cgagcggtga
     6661 gcacgccaaa agtggttcgg cggcactggc tttcccatcg gacgatcaca tgatcgagct
     6721 gtggggaatg ccgatggacg tgcagcgctc cggcgaaatt tcgctgcgtg ggcgggttcg
     6781 tggctgcgcg atgacacccg acggaaagta cctggtgacc ggcgcgagcg acaagctgct
     6841 gcggctgtgg gatttgcgca ccggggccga gctgttgcgc tttgtcggac acactggcgc
     6901 ggtcaatgcg tgctcgattc tgccaggcgg cgtgcacatg gtgacggcgt ctgccgacaa
     6961 gacgctggtg ctgtgggatc tgcgaaccgg cggtgaagtg ctgcgctttt tgggccatgc
     7021 ccagtcggtg agcgcctgcg ccgtgtcgac agacggtcgg cgacttttgt ccggcggcaa
     7081 tgacaaatcg ctgcgccttt gggacttgca gacgggggcc gagcttttgc ggcttctggg
     7141 acacatcgcg ccggtgacag gctgtgcgtt tagcagcgac ggaaaacgtg cggtttcgtg
     7201 ctcgcttgat tacacgctca agctgtggga gctgcacagc gggatgtgtc tggaaactgt
     7261 gtatggaagc gcaccatttc tgtcggtcgt aacgcaaggc gactggctgt gtgccggtga
     7321 tcaggccggc aacgtctgga tgctgcgcga catgacgcct cagtcgacgc agagcggtga
     7381 gccgaactcg cgggcatcga tcgtcgagtc gattcgcaag ctgctgtcgc tgggccgctg
     7441 atctggcggc tgcctgcgtg cgaacgtcaa gccccgcgct gatgtgcgga accggtcagg
     7501 ccggcggaaa atcggcggtc acacgtcggg ccgcagcgat tacggcgcga ccagctcgat
     7561 cgacagatcg tggctttgac tgaggctgag cgagccggtg ccctgacgga cgacgctgcg
     7621 cgcagtgtca aagcctttga tgtcgatggt aatccgctgg gtattatcgg gtacttcgac
     7681 ggcgaaacgt tccatttgct ggtagacgac aaaaccgtcg ctgccacttt cgggaactgg
     7741 gcggctgttt ttggcggcga tatcgtcgag ctgcatggtg acgtaaagct cggtgatgcg
     7801 cggcaaaagg ccgctcactg caacgatgac catgttgtct gggcgcccgc cgcaagatgc
     7861 gacaccgagc gcgagcaaag acgcggccag cagacgacgg aaagacgagt tgcggcgcat
     7921 gaagactctc gctgtgctac ttggttgggt aaagaaccgt cgctggaatg ctgttgcttt
     7981 gggaaacgcc gacggcaatt ccggtgatgg tgactgccgc cgcgacgcca ccgacaaccc
     8041 cccagaacag gggctgcttg taaaacggct tggtcgcagg tttgtcgaca ggcggcgtct
     8101 gggtcagcgc ttggtttgca tcactcggag ctgtcgtcgg aagaatcgac ttcacttccg
     8161 gagctgccgt cgaagttccc ggtgtcggga ccgcggtctg cgccgtattg ccgccagttg
     8221 ccgtcgcagg tgtctgtgtg acagtcgcag cggttttttc gggggccgtc ttggccgccg
     8281 gtgctgtctt gccatcgctc ggcttgctcg tcgaatcgat gtcaaatgga tcgacagtgg
     8341 cgacggaacc gggcgtgacg ttcgatggtg ccgccgcgac cgccgccgca gcagccgcct
     8401 tggctgattt ggcatcgaca gcctcgaagg acggcgggtc aagcatcgcg cgggtcatcg
     8461 cgatgtagcg atcgacttcg gccttgatct ctggtttggg ctttggatcg acgcgcaggt
     8521 agtgttcata aaagcgcagc gccgtcgatg ccatccccag cttgcgatag acctgtccga
     8581 tgttaaggag gatgcgcggt agctgtcgga ttttgtaggc ggcctggaac tcgcgaatgg
     8641 cgcttgggta gtctttgctc tcgtaaaagg cgagtccgcg gctgtagtgc tgctcggcgg
     8701 agtcgccttt ggcagacgcg ctgcgcaccg tcaggctccc gacgaggagc gccagcagca
     8761 aagtgcgcgg gatgtgaccg tagatgccgg caaacagagc agaaatcatc gcttcgcaga
     8821 ttatatcagg aaattctacg ttattttgat taccattgga ttgtcggcac atcgcagcgt
     8881 gattcggacg ctgcagctca gcgacgagca gctacgcacc tgggcgtggc cctcctgtcg
     8941 acccaaggct gagtcgtaag cgcaccgtca cagttggaga gcgaggagca ccgagacgat
     9001 ggaaggacag cgcatcggca actaccgcgt cgtgcgcaag atcgggcagg gcggcatggg
     9061 ctcggtctgg gaagcgaagc acgagcagct gggacgcaag gcggccatca agctgctgcg
     9121 gcgcgagctg accgccgacc cccaggtcga ggtgcgcttt ttcaacgaag ccaaggccgc
     9181 cagccgcgtc gaccatccga gcatcgtcaa aattgatgag ttcggccatc tccccgacgg
     9241 gacggcctac atcatcatgg aatatctgag cggcgagcct ctttcggtgc gactgcgcaa
     9301 cctcggtggc aagctccagc ttcccgacat ctttcgcatc agccggcaga tcgcctccgc
     9361 cctcgccgcc acccacgcca agggaatcat ccaccg
//
LOCUS       DAORGN010000194         9311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3230_length_9311_cov_6.462295, whole genome shotgun sequence.
ACCESSION   DAORGN010000194 DAORGN010000000
VERSION     DAORGN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_9311_cov_6.462295"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1307
                     /locus_tag="PKL17_17435"
     CDS             426..1307
                     /locus_tag="PKL17_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46569.1"
                     /translation="MASRAKLLRLPGHGKLLVASDLHGNYRDFLTIADRFERLGEDGH
                     LLFLGDLVHGPYLEPRSWPTDAPPGHPLWGEPYRDESPAVLLGVQLLSERHPGRVHVL
                     LGNHEHAHIGGPRTALFARDEATLLEQRLGIEASLHMASFFRSLPIVAWAPCGLFFCH
                     AAPAAELIRIDDFENIDYRRYVAPRRSDKLRATKSYKPGDFPARLLGQLLWEHVLPPF
                     KAQSILARVSANIAVYGHTIIPTGYQTIGYEQLILSSSYGMRDGKKTVLLVDLDEHYL
                     TVESLRPGIELLPLYPR"
     gene            complement(1304..1537)
                     /locus_tag="PKL17_17440"
     CDS             complement(1304..1537)
                     /locus_tag="PKL17_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNK46570.1"
                     /translation="MCLALPAQVIALHDDQHATVDLGGVRRKVNVMLVDNLAINDYVL
                     LHVGFAIAKLSPDEAQKTLTLIDEATRSARPAD"
     gene            complement(1544..3802)
                     /gene="hypF"
                     /locus_tag="PKL17_17445"
     CDS             complement(1544..3802)
                     /gene="hypF"
                     /locus_tag="PKL17_17445"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNK46571.1"
                     /translation="MRQRLRIVVRGLVQGVGFRPFVKTHATQLGLTGLVGNDEHGVFI
                     EAQGSNPTALLALLQGGAPKTAQIDSMTTQELPLCDDETQFQIVPSQESAAVQTRIGP
                     DVVPCEACLSEVCDPSSRRYLYPFSSCCDCGPRLTIAHRLPYDRSHTTLALFPLCTAC
                     LAEYHDPQDRRFHAQPMACPVCGPRLSETLSSVVAALRAGQIVALKGVGGYHLLCDAR
                     RDDAVKNLRLRKQRDGKPLAVMVLSVESAKRFAKLSGAESAKLGDPRRPIVLLARRED
                     AGIAPSVASDLSTIGLVLPMSLLHYLLWFEWLGRPTEPQWWLADSDGALVATSGNLHD
                     EPLICDDEEARQKLSPLCDLLVTHDRPIAIRCDDSVVRSIAGQTVFIRRARGYVPDPI
                     PLPVEMPPALGLGGELKATFCLTRGREAFVSQHLGDLDSAATLSAYRETLFHFCRLLD
                     VRPTLCIADRHPDFAARRIATELALPLFEVQHHAAHLHAVMAEQAMCGKALGLCLDGF
                     GLGEDGQAWGGELLFFDGQRVQRLGHLAPILQPGGDLGARQPWRLATAILANLQRPDE
                     ALRRFSARWPVSPLLRIVAQPRLCPSTTACGRYFQAAAALLGVCESESYEAEAAMRLE
                     SLVELPIVQENGYRVLAFEDGRPCQLDLSALWTMLLDCSPGQGAALFHGTLAHGLCAL
                     ALPVLHRLGLRAIVLSGGCVMNRVFTESLCAAFSRHGIAVHLPVRLPPNDGGLSLGQV
                     YAHALGLCAQRD"
     gene            complement(3806..4588)
                     /gene="hypB"
                     /locus_tag="PKL17_17450"
     CDS             complement(3806..4588)
                     /gene="hypB"
                     /locus_tag="PKL17_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNK46572.1"
                     /translation="MCAICGCGSAGTIHDHGGAEDGEHEHVLPDGQVLRHRHAPLQVR
                     TLLVERDLLADNDAVAAHNRFQFAKAKTLVVNLLASPGAGKTALLEKTLHTFAKDCPV
                     AVIEGDQTTSLDAERIRNTGVAAVQVQTGKACHLDAQMVRDALPSLPALPRGLLLIEN
                     VGNLVCPAGFDLGEHKRVVIASVTEGEDKPLKYPAMFATADLLVVSKIDLLPHLDFSV
                     EKLVEHARRIRPGLPVLMLSAKSGAGLADWLHWLADERAHLV"
     gene            complement(4592..4933)
                     /gene="hypA"
                     /locus_tag="PKL17_17455"
     CDS             complement(4592..4933)
                     /gene="hypA"
                     /locus_tag="PKL17_17455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNK46573.1"
                     /translation="MHELALAQSLVRMVVEEAERAQAHSVVTVALQLGALSHVEPSSL
                     SLAFSAASRGTVAHGALLQIEHTLAQAHCLSCGADHSIERRGAGCPSCGAFSLVVVGG
                     DELRLTRLEVL"
     gene            complement(4937..6286)
                     /locus_tag="PKL17_17460"
     CDS             complement(4937..6286)
                     /locus_tag="PKL17_17460"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNK46574.1"
                     /translation="MAGIFKAYDIRGTYPVELDEKLASRIGGAAVSVLGSKRIVIGRD
                     MRQSGPSIRRALIDAILAAGCDVIDIGEVGTPTLYYAVASLSADAGIMITASHNPARY
                     NGFKLCGRGATPISYDTGISAIEQIATSTAQLPVADVPGKLEERNVIADYHQFLLGLT
                     PKTKAFKVVIDTGNGMMGAVLPNLLAQLPCHVTPLFFEVDGSFPNHEPNPLDPRNMVD
                     LQAKVKEVGADLGIAFDGDGDRAMFVDERGELAPSDQITALLAEEFLAKEPGGTVIYD
                     LRSSWVVRDQILSHGGKPLESRVGHSFIKKSMRQTNAIFAGELSGHFYFRDAFFTDNA
                     ELAMLWLLGVLTKRNQPLSQVLKPLACYFATGEVNFVVADKDAVLKKLETSFPGAEVS
                     WLDGITIRLSDWWCNVRPSNTEPVLRLNLEAKTQALRDTMRREVEQLIGGEPATAHH"
     gene            complement(6389..7303)
                     /locus_tag="PKL17_17465"
     CDS             complement(6389..7303)
                     /locus_tag="PKL17_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46575.1"
                     /translation="MRVLPASAALLLFAASVPASAKAPPPPPPPKTAASQVSAESAGR
                     AIGELAGKFKFGMTPEEAMTLMEKDINAKFQPKIEAEKEAADQDRIRKERQDAIDQMR
                     SSYIKFSGQKTGWDVSIVDREFGHRNSESMLVLWEKDLKRFLFFWKEKLYKQYIVYNA
                     EKFKGMDFDTFVGAMEGRYGKASMSFAKKQTDDEMALDYYEWPPQGDYILRAYDATTF
                     YNNFCLSIIQKSQWPQIEKERAVNSPPKVRHTNTHVIDNVTTGDSQTDPNENIVDKML
                     GIKAGASIEQKRIEERVKRQQEKERGGK"
     gene            7463..>9311
                     /locus_tag="PKL17_17470"
     CDS             7463..>9311
                     /locus_tag="PKL17_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46576.1"
                     /translation="MTECTHCRKRYENEVRFCPVDGYAVVPVTEPQKPWVGRLLMGQF
                     QIQSVCGQGATGTVYLATQVGMERQVAVKVLRADLLKDPDIVKRFVREARAGAKISHP
                     NIATVHMVGQTEDGVPFIVMEYVPGRPLSALLDKGKPLSLGKLTQLCSQIAAALTEAH
                     AQGIVHRDLKPENILVSERRGTPDVVKLVDFGIAKILVAYAPGEDAISRMGTVFGTPH
                     YIAPEQASGQAVDGRADLYSLGCILYQMATGRVPFDGKAGLQVLLSQVRDPVVDPREV
                     NPKLSDDLADFILSLLEKDPAARPQTAKEVREQLLALGKGSDKGDDRKGKLTAKGSVF
                     DDDEGDDSGEDDTAASSDDGPSLDAWVSGQQKETPPAPKTRNPPQSARRPASGAAPLP
                     KSGRRADSTPRSVPRSGRPASGRPRPIEAEPARSHRDIIEEDDAVLAEPFYKRHAKPL
                     LGVFGAITVGLLFGVVYAQFRQAPPAPSPVTPPPQVPALAAPATVPIGKPMPAVRPSP
                     SAAKPAAAPAVPTALVPGKPAAPGAPVSASAPLVPATGSLPPTGSLPLAPPTLPAVTP
                     SPAPVAPAVTPTPAPAPAAAATKPAEPTDKTADKPADKPKDNSADKPADK"
BASE COUNT         1873 a         2989 c         2701 g         1748 t
ORIGIN      
        1 ctcggcggtc atcccggcga gctgcgcgtc agactttttc ttccaggtgt cttcgagctg
       61 gtttatgcga accaccagcg gctggagacg gcggacttcg cgttcgtggc ttgtaccaaa
      121 cagcttcttg atgatcgagc taatcatgct tgtgccttga gcgagtgcag aaccgctgcc
      181 acgtcgcgag gtagttcaaa acaaaagagc gggtgaggag tgctgcgaca cgccgcttag
      241 atcatgcggt ccgtgggtat taggcttaag tcgcgctttc tagcagtcaa gattagtaac
      301 ctgccgctcc ctcggagaca agggttaggt tggcgaaaat gctatgatta cagcgcgcct
      361 taggctggca agagtgcgtg cccgctcccc cttaggccag aaacgccaaa tcccgcttac
      421 caaagatggc ctctcgcgca aagctcctac gccttcctgg gcacggaaag ctgctcgtcg
      481 cttctgacct gcacggcaac taccgcgact ttctgacgat agccgaccgc ttcgagcggc
      541 ttggcgaaga cggacacctt ttgtttcttg gcgatctggt gcacggaccg tacctggaac
      601 cgcgaagctg gccgaccgat gcgccgccgg ggcatcctct ttggggcgaa ccgtatcgcg
      661 atgaatcacc cgcggtgctg ctcggcgttc agctcttgtc cgaacgccat ccggggcgcg
      721 tccacgttct tttgggaaat cacgagcacg cgcacattgg cgggccccgc accgcgctgt
      781 ttgcccgcga cgaagcgacg ctgctggaac agcggctggg aatcgaagcg agcctgcaca
      841 tggcgagttt ttttcgatcg ctgccgatcg tcgcgtgggc accgtgtgga ctgtttttct
      901 gtcacgccgc cccggctgcc gagctgattc gcatcgacga cttcgaaaac atcgactatc
      961 gccgctatgt tgcgccgcga cgatccgaca agctgcgcgc caccaagtcg tacaagcccg
     1021 gtgattttcc ggcgcggctg ctcggacagc tgctctggga acatgtgctg ccgccgttca
     1081 aagcgcagtc gatcctcgcc cgtgtcagcg ccaacatcgc ggtctatggt cacacgatca
     1141 ttccgacggg atatcaaacg atcggctatg aacagctgat tttgtcgtcg agctatggga
     1201 tgcgtgacgg aaaaaaaacg gtgctgctgg tcgaccttga tgagcactat ctcaccgtcg
     1261 agagcctgcg ccccggcatc gaactattgc ccctgtatcc gcgctagtcc gcaggtcgtg
     1321 ccgatcgggt cgcttcgtcg atgagcgtca gcgtcttttg cgcttcatcg ggacttagct
     1381 tggcaatcgc gaaaccgacg tgcaaaagca cgtagtcgtt tatcgccagg ttgtcgacga
     1441 gcatcacgtt gacttttcgt cgcacaccgc ccaaatcaac cgtcgcatgc tggtcatcgt
     1501 ggagcgctat cacttgcgcc ggcagggcca aacacatcgc cgttcaatcc ctctgcgcac
     1561 agaggccgag cgcatgggcg tagacctggc cgagcgacag accgccatcg tttggcggca
     1621 gccgcacagg caaatgcacc gcgatgccat gacgggaaaa cgctgcgcaa agggactcgg
     1681 tgaacactcg attcatcaca caaccgccgc tcagcacaat tgcgcgcaaa cccaagcgat
     1741 gcaagacggg aagagccagc gcacaaagtc cgtgggcgag cgtgccatga aacagcgccg
     1801 cgccctgccc tggcgaacag tcgagcagca ttgtccacag cgccgacaga tcgagctgac
     1861 aaggccgccc gtcttcaaac gccagcaccc ggtagccgtt ttcctgcacg attgggagtt
     1921 cgaccagcga ctccagccgc atcgccgctt cggcttcata cgattcgctt tcgcacaccc
     1981 ccaaaagcgc cgccgccgcc tgaaaatatc gaccacacgc cgtcgtcgat ggacaaagcc
     2041 gtggctgcgc gacgatgcgc aatagcggcg acaccggcca gcgcgcagaa aaccgccgca
     2101 gcgcttcgtc tggccgctgc aggttcgcca aaatcgccgt cgccagtcgc cagggctgtc
     2161 gcgcccccag atcgccgccc ggctgcaaaa tcggcgccag gtgccccagt ctttgtacgc
     2221 gctgtccgtc gaaaaacaag agctcgccgc cccatgcctg gccatcctcg ccaagcccaa
     2281 aaccgtcgag acaaagcccg agcgccttgc cgcacatcgc ctgttccgcc atcaccgcat
     2341 gaagatgggc agcgtggtgc tggacttcga aaagtggcaa ggcaagctct gtcgcaatcc
     2401 ggcgcgcggc aaagtcggga tgcctatcgg caatacacag cgtcggacgc acgtcgagca
     2461 gacgacaaaa atgaaacagc gtctctcgat acgccgaaag cgtcgctgct gaatccagat
     2521 cgcccaggtg ttgcgaaaca aacgcctcgc gtccgcgggt cagacaaaac gtcgctttca
     2581 gctcaccgcc caggcccaaa gcgggaggca tctcgacggg aagtgggatc ggatcgggca
     2641 cataaccgcg cgcgcgtcga atgaacaccg tctgacccgc gatcgagcgc accacactgt
     2701 catcacagcg gatcgcgatc ggacgatcgt gggtcacaag caagtcgcag agcggcgaaa
     2761 gtttctgcct ggcttcttca tcgtcacaaa tcagcggctc gtcgtgcaga ttgcccgatg
     2821 ttgccaccag cgccccatca gaatcggcga gccaccactg cggttccgtc ggacgcccca
     2881 gccattcaaa ccacaaaagg tagtgcaaaa gcgacatcgg cagcacaagg ccgatcgtcg
     2941 acaaatcgct ggccacggaa ggtgcaattc cagcgtcttc acgccgcgca agcagcacaa
     3001 tcggacgccg cggatcgcca agcttggccg attccgcgcc cgacaacttg gcaaaccgtt
     3061 tggcactttc gacgctcagc accatgaccg cgagtggttt gccgtcgcgc tgtttgcgca
     3121 gtcgcaggtt ttttaccgca tcgtcccgtc gcgcatcgca caaaaggtga tacccgccga
     3181 ccccttttag cgcgacgatt tgcccggcgc gcagcgcggc aaccaccgac gaaagcgttt
     3241 cgcttagcct aggaccgcag accgggcaag ccatcggctg cgcatggaag cgacggtctt
     3301 gcggatcgtg atactcggcc agacacgcgg tacagagcgg aaagagagcg agcgtggtgt
     3361 ggctgcggtc atacggcaga cgatgcgcga tggtgagccg tggaccacag tcgcagcagc
     3421 tcgaaaacgg atacaagtag cgacgcgacg acggatcgca gacctctgac aagcaggctt
     3481 cacacggcac aacatctggc ccgatgcgcg tctgcaccgc tgctgattcc tgacttggca
     3541 caatctgaaa ctgcgtttca tcgtcgcaga gcggcagctc ttgggtcgtc atcgaatcaa
     3601 tctgcgcggt cttcggtgca ccaccttgca acagcgcaag cagcgccgtc ggattcgacc
     3661 cttgcgcttc gatgaagacg ccgtgctcgt cattgccgac gagtcccgtc agcccgagct
     3721 gtgtcgcatg ggtctttaca aacggacgaa acccgacccc ttgcacaagg ccgcgcacca
     3781 caattcgcag ccgctgtctc atcggctaga ccagatgtgc ccgctcgtca gccagccagt
     3841 gcagccagtc cgcaagacct gcgcctgact tcgccgacaa catgagcacc ggcagaccgg
     3901 gccgaatccg ccgcgcatgc tcgaccagtt tttcgacgga aaagtccaag tggggcagca
     3961 gatcgatttt gctcaccacc agcagatccg ccgtcgcaaa catcgccgga tatttcagcg
     4021 gcttgtcttc gccctccgtc accgacgcaa tcaccacccg cttgtgttcg cccaggtcga
     4081 agcccgccgg gcagaccaag ttgcccacat tttcgatcag caaaaggcca cgcggcaacg
     4141 ccggcaagct cggcagcgca tcacgcacca tctgcgcgtc gagatgacac gcttttcccg
     4201 tctgaacctg gaccgctgcg acgccggtgt tgcgaattcg ctctgcatcg agactggtcg
     4261 tctgatcgcc ttctatcacg gcgaccggac agtcttttgc gaatgtatgc agcgtctttt
     4321 caagcagcgc cgtcttgcct gcccccggac tcgccagaag gttcaccacc agcgtcttgg
     4381 ccttggcaaa ctgaaagcga ttgtgcgcag cgacggcgtc attgtcagcc aacagatcgc
     4441 gctcgacgag cagggtgcga acctggagcg gcgcatgccg gtgacgcaac acctgaccgt
     4501 ctggcaaaac gtgctcatgt tcgccgtctt ctgcgcctcc atgatcgtgg atagtcccgg
     4561 cgctgccaca gccgcagatc gcacacatcg cttacagaac ctccaagcga gtgagccgta
     4621 gctcatcgcc gcccacgacg acaagagaga acgcaccgca cgaaggacaa ccggcgccac
     4681 ggcgctcgat ggagtggtcc gcgccacacg acagacagtg ggcctgcgcc agcgtgtgtt
     4741 caatctggag aagggcccca tgcgcgaccg taccgcggct cgctgcggaa aaagccaggc
     4801 ttagcgacga aggctcgacg tgggacagcg ccccgagttg aagcgcgact gtcaccacgc
     4861 tgtgggcctg tgctcgctcg gcttcttcga cgaccatgcg aaccagggac tgggccagcg
     4921 caagctcgtg catcggtcag tgatgtgccg ttgcaggctc gcccccgata agctgctcaa
     4981 cttctcgccg catggtgtcg cgcagcgcct gggtcttggc ttccaggttc agacgcagca
     5041 ccggctcggt attgcttgga cggacgttgc accaccagtc ggaaagacga atcgtaatgc
     5101 catcgagcca cgacacttcg gcgccaggga acgaggtttc cagcttcttt agcaccgcat
     5161 ctttgtcagc gacgacaaag ttaacttcac ccgtcgcaaa gtaacaagcg agcggcttta
     5221 gcacctggct gagcggctga tttcgcttgg tcaaaacccc aagcagccac agcatcgcca
     5281 gctccgcatt gtcggtaaaa aaggcatcgc ggaagtaaaa gtgccccgac agctcgccgg
     5341 caaagatcgc gtttgtctgc cgcatgctct ttttgatgaa gctgtgaccg acgcggcttt
     5401 cgagcggttt gccaccatgc gaaaggatct gatcccgcac aacccacgac gagcgaaggt
     5461 cgtaaatcac cgtaccgccc ggctctttgg ccaaaaactc ttccgccaaa agcgccgtga
     5521 tctgatcgct cggtgccagc tcgccacgct cgtcgacaaa catcgcccga tcgccatcgc
     5581 catcaaacgc aatgcccaga tcggcgccga cttctttgac cttggcttgc aggtcgacca
     5641 tgttgcgcgg atcgagcgga ttgggctcgt ggttggggaa gctgccgtcg acttcaaaga
     5701 acagcggcgt tacatgacac ggcagctgcg cgagcagatt gggcagcacc gcccccatca
     5761 tgccgttgcc ggtgtcgatg acgactttga acgccttggt tttcggcgta agcccgagca
     5821 aaaactgatg gtaatccgcg atgacgttgc gctcttccag cttgcctggc acatccgcga
     5881 cggggagctg cgcagtcgaa gtcgcaatct gttcgatcgc tgaaatcccg gtgtcgtaac
     5941 ttatcggcgt cgcgccgcgt ccgcacagct tgaagccgtt gtagcgggcg ggattgtgac
     6001 tggcggtgat catgatcccg gcgtcggcag aaaggctggc gacggcgtaa tacagcgtcg
     6061 gcgtgcccac ttccccaatg tcgatgacat cgcagccggc ggccaaaatg gcgtcgatca
     6121 gggcacggcg aatgctcggg ccgctctgtc gcatgtcccg tccgatgacg atgcgttttg
     6181 atccgagtac cgacaccgca gcgccgccaa ttcgagaagc caacttctca tcgagctcga
     6241 cgggataggt gccgcgaatg tcatacgcct tgaaaatgcc tgccatggtt gtccttcggg
     6301 aatgcatcgg ctgcttgtgc cgcgcgggat ggcggcgctg ccgaggtctt tgcggttgtg
     6361 ctggaaaaac gagagcgatc gacgcggact acttgccgcc gcgctctttt tcttgctgac
     6421 gcttgacgcg ctcttcgatg cgcttctgtt cgatgctggc gccggccttg atcccgagca
     6481 tcttgtcgac gatgttttcg ttcggatcgg tctgagaatc gccggtcgtc acgttgtcga
     6541 tgacgtgggt gttggtgtgg cgcactttcg gcgggctgtt gacggcgcgc tctttttcga
     6601 tctgcggcca ctgcgacttc tgaatgatcg acagacagaa gttgttgtaa aacgtcgtcg
     6661 catcgtaggc gcgcaggatg tagtcacctt gcggcggcca ctcgtagtaa tcgagcgcca
     6721 tttcatcgtc ggtctgcttc ttggcaaagc tcatcgaggc cttgccgtag cgaccttcca
     6781 tcgcgccgac gaacgtatca aagtccatgc ctttgaactt ttcggcgttg tagacgatgt
     6841 actgcttgta gagcttttcc ttccagaaga agaggaagcg cttgagatcc ttttcccaca
     6901 gcaccagcat cgactcgctg ttgcggtggc caaactcgcg gtcgacgatc gacacgtccc
     6961 agccggtctt ttgaccgctg aacttgatgt acgagctgcg catttggtcg atcgcgtcct
     7021 ggcgttcttt gcggatgcga tcttgatcgg cggcttcttt ttcggcctcg atcttgggtt
     7081 ggaacttggc gttgatgtcc ttctccatca acgtcatcgc ctcttccggg gtcatgccga
     7141 acttgaactt gccggccagt tccccgatcg cgcgaccggc ggactcagca gacacctgcg
     7201 aggcggcagt cttcggcggc gggggcggcg gcggggcctt ggcgcttgcg ggaacactcg
     7261 ccgcgaaaag cagcagcgcg gcagaagcag gaaggactct catcgtcgaa tttctcctgt
     7321 cgattcaagt accacaccac gtagagcgac gccatatgcc aagcggctcc gtcgcgtatg
     7381 gctggtccga tcgtcatctg cgatcatgga tggtctgacg accgagcttt tgagtcgttc
     7441 accgaagtgc taaaaacaag acgtgaccga gtgcacccac tgccgaaaac ggtatgaaaa
     7501 cgaagtccga ttctgtccgg tcgacggtta tgcggtcgtg ccggtcacgg aaccgcagaa
     7561 accctgggtc ggacggctgc tgatgggcca gttccagatc cagtcggtct gcggacaagg
     7621 agcgacagga actgtttatt tggcgacgca agttgggatg gaacgccaag tcgccgtcaa
     7681 agtcttgcgc gccgatctcc tcaaagatcc cgacatcgtc aagcgcttcg tccgcgaggc
     7741 ccgcgccggt gccaaaatca gccatccgaa catcgccacc gtccacatgg tcgggcagac
     7801 cgaagacggc gtgccattca tcgtcatgga gtatgtgccg gggcgaccgc tttcggcgct
     7861 gctcgacaaa ggcaagccgc tttcgctcgg caagctgacc cagctttgca gccagatcgc
     7921 ggcggcgctc accgaagcgc atgcgcaggg catcgtccac cgcgatctca aaccggaaaa
     7981 cattctcgtc agcgagcgac gcggcacacc cgatgtcgtc aagctggtcg acttcggcat
     8041 tgccaaaatc ctcgtcgcgt atgcaccggg ggaagatgcg atctcgcgga tgggcacggt
     8101 gtttggaacg ccgcactaca tcgcgcccga gcaagcttcc ggacaagccg tcgatggtcg
     8161 cgccgatctg tactcgcttg gctgcatcct gtaccagatg gcgacggggc gcgtgccatt
     8221 cgatggcaaa gccggcctgc aagtgctctt gtcacaagtt cgcgatccag ttgtcgatcc
     8281 gcgtgaggtc aatcccaagc tgtccgatga cttggctgac tttattttga gcctgctcga
     8341 aaaagacccg gcggcgcgtc cgcagaccgc caaagaagtc cgggagcagc tccttgcgct
     8401 tgggaaaggt tccgacaaag gcgacgatcg aaaaggcaag ctcaccgcca aaggttcggt
     8461 atttgacgac gacgaaggcg atgacagcgg cgaggatgac accgcagcat cgagtgatga
     8521 cggaccgtcg cttgatgcct gggtaagtgg ccagcaaaaa gaaacgccgc cagccccaaa
     8581 gacgcgcaac cctccgcagt cggcccgtcg tccagcaagt ggcgccgccc cgctgcccaa
     8641 gagcggtcgt cgcgccgatt cgacgccccg cagtgtgccg cgctcgggtc gtccggcaag
     8701 tggtcgccca cgccctatcg aagccgagcc cgcccgctcc catcgcgaca tcatcgaaga
     8761 agacgatgcc gtcttggccg aaccgtttta caagcgacac gccaagccgc tcctcggtgt
     8821 gtttggcgcc atcacggttg gcctgctgtt tggggtggtc tatgcccagt tccgccaggc
     8881 accacccgcg ccaagcccgg tgacaccacc gccacaggtt ccggccctgg ctgccccggc
     8941 gactgtaccg atcggcaaac cgatgcccgc ggttcgccca agtccaagtg ccgccaagcc
     9001 ggctgcggcc cccgccgtgc ccacggcttt ggtcccaggc aaaccggcag ccccaggcgc
     9061 cccggtttcg gcttctgccc cccttgttcc ggcgacgggt tcgctgccac cgaccggaag
     9121 tttgccactc gcaccgccga ccctgccggc tgtcactccg tccccggccc cggttgcacc
     9181 agctgtgacg cccacgcctg cgcccgcccc ggctgctgct gcgaccaaac ccgcagagcc
     9241 cacggacaaa accgccgaca aacccgctga caaaccgaaa gacaacagcg cggacaaacc
     9301 tgctgacaaa c
//
LOCUS       DAORGN010000195         9303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3236_length_9303_cov_6.930147, whole genome shotgun sequence.
ACCESSION   DAORGN010000195 DAORGN010000000
VERSION     DAORGN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3236_length_9303_cov_6.930147"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKL17_17475"
     CDS             complement(<1..337)
                     /locus_tag="PKL17_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HNK46577.1"
                     /translation="MATSYLSPGVYVEEVDRGSKPIEAVGTNTVGFLGESMMGPVNQA
                     VLVTNWTQYVKTFGDFTQSTFLSHAVYGFFNNGGSRCYVVNVGAPANLDLSPADTKAA
                     AEKAAAVKAD"
     gene            complement(472..1101)
                     /locus_tag="PKL17_17480"
     CDS             complement(472..1101)
                     /locus_tag="PKL17_17480"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HNK46578.1"
                     /translation="MNPGQYHIIKETSQSIGELLKQQYKQIGYKRVHLVVAAPKQENI
                     EGKMPAVSLYLYNITLDEEGVGNNRSGQYIDVEFGSDGQAREIARDLPMWLRLDYLIS
                     CWAQTPEEEQLLLGATIRTFMEHPTIRGEQLKGDSFEPDDYIPLLLSQKLDETVLSRF
                     WSSLNQPLKPAVQCWTTIPLYPTTSRTFTRVREREVRFFDLDKLNRLRQ"
     gene            complement(1666..4293)
                     /gene="topA"
                     /locus_tag="PKL17_17485"
     CDS             complement(1666..4293)
                     /gene="topA"
                     /locus_tag="PKL17_17485"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNK46579.1"
                     /translation="MAKAKSSTTSKAARKTTSSDEESTSAPKKSTKASAKKASPAKKS
                     AAKSKAPSEKETKSTSKKSTASKKKPTKKAAASKKSTPARGGGKRGSMLVVVESPAKA
                     KTIEKYLGGAAKVMASVGHVKDLPKSKIGVDLENDFTPEYVVIRGKGKILSELCKAAK
                     QSDVVYLAPDPDREGEAIAWHLADEIRTANENIKRVLFNEITQRGITEAIANPTDLDK
                     HKYDAQQTRRVLDRLVGYQISPILWNKVRRGLSAGRVQSVAVRIVVEREAEIDAFTAE
                     EYWTVQAEVEGKTPPRFYLRLYKINDKKPDRLDGATAQQIAALLRKAPLKVAQVERKE
                     RRKLPTAPFITSKLQQEAARKLRFTAKRTMGVAQRLYEGVELGSEGRVGLITYMRTDS
                     TRISADALTEVRGYITQRFGKEALPDEPVVYKSKKGNVQDAHEAIRPTSTKYDPETVR
                     KLLREEAAKNPEKARDIDDQIKLYQLIWNRFVASQMKPALYDQTTAIVEVKDAGQSYE
                     LRASGQVLRFPGFTLIYTETEDEDSPQVDQGADGDRLPSLQQGELLKALSVAADQHFT
                     VPPPRFTEASLVKELEEKGIGRPSTYATILSTIQDRGYVEKKEGRFHPTDLGKRVNEL
                     LVQSFPDILNVDFTAKMETDLDEVEEGQREMLALLTEFYGPFKTDVEKAHVEMKDLKK
                     VEIPTELQCEKCGQTMVIKWGRNGEFLACSGYPECKNTKEFTRKKDGTIELVPEPTTD
                     EKCPTCGADLIFRRGRFGEFWACSRYPECKTTRPVSLGITCPKPGCGGFLTERRSRRG
                     SPFFGCSNYSAKGCDFVSWDRPIKEPCPDCGAPFLLKKQNRSGTKLRCASCSYQSEPA
                     ESDMPDVGGEDSSSEEDAA"
     gene            complement(4301..5284)
                     /locus_tag="PKL17_17490"
     CDS             complement(4301..5284)
                     /locus_tag="PKL17_17490"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNK46580.1"
                     /translation="MNALSPLASYREKPDAQPLVDGARQCLPSDADQPARLRLWRKPQ
                     AFWLRGQAQLLDGRPLCTVVGSRACSRVAEERAFLLGQKLARAGVVVVSGGALGIDAA
                     AHRGALSVSGATCAVVGTGIDVYYPKQHATLLLEIAEKGLLLSMFAPGAPPLPLHFRI
                     RNELMAAIADVVVVVEAQERSGSLITARQALRHGRRLCALTGSAGTARLLESGAKLAH
                     SVDEVVDFVTASGGDAHQTAAAVAAPSDVAESRAVATAVTAEALHLGCEEAAARVLSA
                     LAACAPADVGELCARTGLCAAECAAVLVDLELADRCTRLAGGRYIVHAPLS"
     gene            5805..6899
                     /locus_tag="PKL17_17495"
     CDS             5805..6899
                     /locus_tag="PKL17_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46581.1"
                     /translation="MTASSRPGFRQISTEPALDHAAKVGGYAWWYAEVHDAEDQFGLT
                     LIVFAGSVFSADYAQRLRRGQPITGLDVPAVNLAVYKRRGPGGPSTQKLWVMNEYGKA
                     ALRTDAQLIAVADSQLSVQSDGSLQVQLHEPTTRFFGKPGAVVDLSLRLGPPTQVPAP
                     LVLGHSPRGEAHLWQPFSLLSPAEVELRLPNERICFSGTAYADHNYGSGRLEDCFARW
                     GWAHGFSDDGSCGAVVYDTTALDGQRRRIGLLASGRDALPIVEEFAEPPPPSEPGRDG
                     RDFWWQKVPRAMSAGSLHCTRREHGRLLDAPFYARFGVEFVDEARVPGIKLRGVGEYL
                     DLERFRLPALQFLLRYKTYVARRSDDSQAE"
     gene            7033..8175
                     /locus_tag="PKL17_17500"
     CDS             7033..8175
                     /locus_tag="PKL17_17500"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK46582.1"
                     /translation="MLLLFGVLRLRKTPPGPPPAWPAIVVLRPCEGDEPGLYENLLSS
                     CLAPYEGPRRVLLLCPSTSDPSYPLLLRVAKAAPPTIPVEVLLTRPEALHNRKVSQLL
                     VGIARSTEPILITADSDVLLGEKDLPSLVSLLCAPPSTPVQRQQVGIAFAAPVEQQPR
                     TRWDRLSSALVGGSVQNFMALYGLYRLIGGVPSMAGALLAISRRALLDIGGLQEVRTI
                     LGEDHELARRLCAAGYSVEQSPFPAHCSDGERSLYGVISRVARWLMVVRAQRPLLLLT
                     YPLFMAATPPLLVATAILRTPLLSLLAGLIFTARTLLSWLLRRAQHVQQGFFESALEV
                     IGAELLLWTGLAKALATRQINWRGYRFRIARGGHMIPEAQAPSAQP"
     gene            complement(8206..8901)
                     /locus_tag="PKL17_17505"
     CDS             complement(8206..8901)
                     /locus_tag="PKL17_17505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNK46583.1"
                     /translation="MPTVGGLGPDFALYNQDQHMVQLGRLQSEADFVVVYFFPQAGAP
                     GCAREAIGFRDIVPELAKRRVKVVGITASPASEITAFAKEHNLNFDVLCDSMRRISEE
                     WGALRNDNTARMTFIVNAKGVITHMFPRVDVWKHAAEVLTLFPQSSEAAATETPASTT
                     AQAPAQVASSNAPAAAQAAAHPPVSAGHSAGPDLVAEAARAVLKLLLMHKQSGGAIPP
                     DVSELLAQLGRNA"
BASE COUNT         1665 a         2859 c         2802 g         1977 t
ORIGIN      
        1 cgtcagcctt cacagccgcg gccttttccg cagcggcctt ggtgtccgcc gggctcaggt
       61 cgaggttcgc tggcgcgccg acgttcacaa cgtagcagcg cgagccgccg ttgttgaaga
      121 agccgtagac ggcgtgcgac aggaaggtgg actgggtgaa gtcgccgaag gtcttgacgt
      181 actgggtcca gttcgtgacg agcactgcct ggttgacagg acccatcatc gactcgccca
      241 ggaagccgac cgtgttggtt ccaactgcct caatcggctt tgaaccacgg tcaacttctt
      301 caacgtatac gcccggcgaa aggtaactag tggccatgct gcgatctcct tgctgatttg
      361 cctaccgtca gatgatcccc agggttgcct tccgcgctgg tccaagtggc ttcgcagaag
      421 gttatcggta cggcctggcg atggttgctt tccaatggcc tcgtcagttc ctcactgacg
      481 aagccggttc agtttgtcca aatcgaaaaa gcggacttcg cgctcgcgca cccgcgtgaa
      541 cgtccgcgag gtcgtcggat acagcgggat cgtcgtccaa cactgcaccg ccggcttcag
      601 cggctgattc aggctcgacc agaagcggga caacaccgtt tcatccagct tctgggaaag
      661 cagaagcgga atgtagtcgt ccggctcgaa ggagtcgcct ttgagctgct cgccgcgaat
      721 cgtcggatgt tccatgaagg tccggatcgt cgcgccgagc agaagctgtt cttcttctgg
      781 cgtctgcgcc cagcacgaga tgagatagtc gagacgcagc cacatgggca ggtcacgcgc
      841 gatctctcgt gcttgtccgt cagaaccaaa ctcgacgtcg atgtactggc cgcttcggtt
      901 gttgccgacg ccttcttcat cgagcgtgat gttgtacaag tagagagaca ccgcgggcat
      961 cttcccctcg atgttctcct gcttgggtgc agcgacgacc aaatggacgc gcttgtagcc
     1021 aatctgcttg tactgctgct tcaggagctc cccgatggat tgggaagtct ccttaatgat
     1081 gtggtactgg cctggattca tggcctgccc gactgccctt gcttcccccc ctgagatagc
     1141 tctgatcgtc agagctaagg tccagggtca gcctacgtta gtggatcgga agggtttctg
     1201 tcaatccttc attttccctg ccgccggaca tcaataccga cgagaaaccc acgcgatcac
     1261 tacgaaatgt ggccagggca tctgactgca aaaggagtcg ttttttgcag cgaaaggagt
     1321 cgtttgggag gacgaattcc caatgtagga atcgcattac ctaccgacgg aaatgggaac
     1381 tgaaattccg aattctgtaa cctgcggatc tgcttgtctg aatgcgcgaa agaatcgacc
     1441 agcggcgggc atcaaggccg gacgatcaat attagtcatg cgaaatgact aatttataag
     1501 ggatgtaccg atcaggtcca accgaatggc agatgaccgc ttggcgccgg cacaagcggg
     1561 gccttgggcg atcggaccat catcgagctg gcggtgaaaa aaggacggat cggcgacgga
     1621 aaggtgggac ttgggggcaa gcgagccagg acgaaaagtg cgcggttagg ccgcgtcttc
     1681 ttcgctgctg gaatcctcgc cgcccacatc aggcatatcg gattcggcgg gttcgctctg
     1741 gtagctacac gaagcgcagc gcagctttgt acccgaccga ttctgcttct tcagcaggaa
     1801 gggtgcgcca cagtctggac acggctcctt gattggccta tcccacgaca caaagtcgca
     1861 gcctttcgcg ctgtagttgg agcagccgaa gaacggcgag ccacgtcgcg agcggcgttc
     1921 ggtcaagaag ccaccgcagc cgggtttcgg acaggtgatg cccaagctca cgggacgggt
     1981 ggtcttgcac tcgggatagc gcgagcaggc ccaaaattca ccaaagcgac cacggcggaa
     2041 gatcagatcg gcgccgcagg tcgggcactt ttcgtcggtg gtcggctcgg ggaccagctc
     2101 gatggtgccg tcttttttgc gcgtaaactc tttggtgttt ttgcactcgg gatagccact
     2161 gcaagcgaga aactcaccgt ttctgcccca cttgatgacc atcgtctggc cgcacttttc
     2221 gcactgaagc tccgtcggaa tttcgacctt tttgaggtct ttcatctcga cgtgggcttt
     2281 ttcgacatcg gtcttgaacg ggccgtaaaa ctcggtcaaa agcgccagca tctcgcgctg
     2341 accctcttcg acctcgtcca gatcggtttc catcttcgcc gtaaagtcga cgttgaggat
     2401 gtccgggaag ctctgcacca gcagctcgtt tacacgcttg cccagatccg tcggatggaa
     2461 gcggccttct ttcttttcga cgtagccgcg atcctgaatg gtcgacagaa tcgtcgcata
     2521 cgtcgacgga cggccaatcc ccttctcttc cagttctttg acgagcgacg cttcggtaaa
     2581 gcgcgggggc ggcaccgtga agtgctgatc ggcagcgacg cttagcgcct tgagcaactc
     2641 gccctgctgg agactcggca gccgatcgcc atcggcccct tggtcgacct gcggcgagtc
     2701 ttcgtcttcc gtctcggtat agatcagcgt gaagccgggg aagcgcagca cttggccact
     2761 cgcgcgcagc tcgtagctct ggccggcgtc tttgacttcg acgatcgcgg tcgtctggtc
     2821 gtagagcgcc ggcttcatct gcgacgccac aaagcggttc cagatgagct gatagagctt
     2881 gatctgatcg tcgatgtcgc gcgctttttc ggggtttttc gccgcttctt cgcgcaaaag
     2941 cttgcgcacc gtttcgggat cgtacttggt gctggtcgga cggatcgctt cgtgcgcgtc
     3001 ctggacgttg ccctttttcg acttgtagac cacgggctcg tcgggcagcg cttctttgcc
     3061 gaatcgctgg gtgatgtaac cacgcacttc agtcagcgcg tcggcactga tccgcgtcga
     3121 atcggtacgc atgtaggtga tcaagccgac gcggccttcg ctgcccagct cgacaccttc
     3181 ataaagccgc tgcgcgacac ccatcgtccg cttggccgta aaccgcagct tgcgcgccgc
     3241 ttcctgctga agcttgctcg tgataaacgg cgccgtcggc agcttgcgcc gctctttgcg
     3301 ttcgacctgc gcaaccttga gcggcgcttt gcgcaacagc gcagcgatct gctgagccgt
     3361 cgccccatcg agccgatcgg gctttttgtc attgatcttg tagagccgca ggtaaaagcg
     3421 cggtggcgtc ttgccttcga cttcggcctg cacggtccag tattcttcgg cggtaaatgc
     3481 gtcgatttcg gcttcccgtt cgacgacaat gcgcaccgct accgactgaa cccggcccgc
     3541 cgacagaccg cgccggacct tgttccacaa gatcggcgaa atttgatagc cgaccaagcg
     3601 atcaagcacg cggcgcgtct gctgggcgtc gtacttgtgc ttgtcgaggt cggtcgggtt
     3661 ggcgatcgcc tcggtgatcc cgcgctgcgt gatttcatta aaaagcacgc gcttgatgtt
     3721 ttcgttcgca gtgcgaatct cgtcggccaa gtgccaggcg atcgcttctc cttcgcgatc
     3781 agggtcaggc gccaggtaca cgacatcgct ctgcttggcg gccttgcaca gctcgctgag
     3841 gattttgccc ttgccgcgaa tgacgacgta ctcgggggta aagtcgtttt ccaggtcgac
     3901 gccgatcttc gatttgggca ggtctttgac gtgacccacc gacgccatga ccttggctgc
     3961 accacccaaa tatttttcga tggtcttggc cttggcagga gactcgacca ccaccagcat
     4021 cgatccgcgc ttgccacctc cgcgtgcggg cgtcgacttt ttgctcgcag cggccttttt
     4081 ggtcggcttt ttcttgctcg cagtcgactt tttgctcgtc gacttggtct ctttttcgct
     4141 tggcgccttg ctcttggcgg cgctcttttt cgccggactc gctttcttcg ccgacgcttt
     4201 ggtcgacttc ttgggggcgg aggtactctc ctcgtcagac gatgtggtct ttcgagcagc
     4261 cttagaagtg gtcgaggact tggctttggc catggctttc ctagctaagc ggagcatgca
     4321 cgatgtaccg cccccccgcc aagcgggtac agcgatcggc aagctccaag tctacgagta
     4381 ccgccgcgca ctcagccgca caaagaccgg tacgggcaca cagttcccct acatctgcgg
     4441 gcgcacaagc cgcgagcgcc gaaagcaccc gcgccgccgc ttcttcgcag cccaaatgca
     4501 gcgcttctgc cgtcacagcc gtcgctacgg cccggctttc ggcgacatcg gatggcgcag
     4561 caacagccgc cgcagtttgg tgtgcatcac cgccagacgc ggtgacaaaa tcgacaactt
     4621 catcgacgct gtgcgcaagc ttggcaccgg attccagcag cctggcagtt cctgccgatc
     4681 cggttagcgc acacagccgt cgtccgtggc gcaacgcctg tcgcgccgtg ataagcgatc
     4741 ccgatcgctc ttgcgcctcg acaaccacca cgacatctgc gatcgccgcc atcagctcgt
     4801 tgcgaatgcg aaagtggagc ggcagcggtg gggcccccgg cgcaaacatc gacaacagca
     4861 gccctttttc cgcgatctcc aaaagcaggg tcgcatgttg cttggggtaa tacacgtcga
     4921 tgccggttcc aaccaccgcg caggtcgcac ccgaaaccga cagcgcgccg cgatgggccg
     4981 cagcatcgat cccgagcgcg cccccggaaa ccaccacaac ccccgcgcga gccagctttt
     5041 gcccaagcaa aaacgcgcgc tcttctgcta cccgactgca cgcccgcgaa ccaaccaccg
     5101 tacacagcgg acgaccgtcg agaagttgag cctgcccgcg cagccaaaat gcttggggtt
     5161 tccgccacag ccgcagcctc gcaggttggt ccgcatccga tggcaggcac tgtcgcgccc
     5221 cgtccacaag cggttgggca tccggctttt ctcgatatga agcgagcggc gacaaggcat
     5281 tcacaagtag gcactccctc gcagcgcgtc aaggctcatg cttgtttccg acggagaaat
     5341 ggacgccaga ccagcgcaag cccggaccgt gatgcggcaa tttgcagtca acccggccac
     5401 cacaaaggcg caaaccctcg cacacgcagc ccgcagcagc ggttcatttg ccgtattcat
     5461 gcgtaattat ttgtattttg gatttctttt ttcatgcatt ctattgtcct cgctttaggt
     5521 cgtgaccagc ccaggcggtc gttggcgcac tcgctgccgg ggggcccgca agcaccacac
     5581 ttcacggtgc gggcgggaaa agagcagcgc tgccagcgcc accaagtgac catcgggggc
     5641 cctgggctgg tcacgtccgc gcgaggtttg gcggcctttt tggccaaagc cagcgtgcgg
     5701 cgcagcgtct ttggcggacg cagcatcgaa accagttgcc cgtggtgaag cgatccgctt
     5761 tgctgcgagc ggcctttcgc catcgcatgg aggctgtttt tctcgtgacc gcttcgtctc
     5821 ggccaggctt tcggcaaatc tctacggaac cggcgctcga tcatgccgcc aaagtcggcg
     5881 gttatgcctg gtggtacgcc gaggttcacg acgctgaaga tcagtttggc ctgacgctga
     5941 tcgtctttgc cggatcggtg ttttcagccg actatgccca gcgcctgcgt cggggtcagc
     6001 cgatcaccgg cctggatgtg ccggcggtca acctggccgt ttacaagcgc cgcggccccg
     6061 gtggaccgtc gacccagaag ctgtgggtga tgaacgaata cggaaaggcc gcgctgcgca
     6121 ccgatgccca gttgatcgcg gtcgcggaca gccaactttc ggtgcagagc gatggcagcc
     6181 ttcaggtgca gctgcacgaa ccgacgacgc gattttttgg caaaccgggc gcggttgtcg
     6241 atctctcact gcggcttggc ccgcccacgc aagttcctgc gccgcttgtg ctcgggcaca
     6301 gtccgcgtgg cgaagcgcac ttgtggcagc cgttttcgct tctgtcgccc gccgaggtcg
     6361 agctgcgact gcccaacgag cgcatctgct tttccggcac ggcctacgcc gatcacaact
     6421 acggatcggg acggctggaa gactgctttg cccgctgggg ctgggcgcac ggtttttccg
     6481 acgatggaag ctgtggagcg gtcgtttatg acaccaccgc actcgatgga cagcgacgtc
     6541 gaatcggact gcttgccagc ggacgtgacg ccctgccgat cgtggaagag tttgccgaac
     6601 cgccgccgcc gagcgagcca ggccgcgacg gtcgcgactt ttggtggcag aaagtgccgc
     6661 gcgcgatgtc cgccggatcg ctgcactgta cccgaaggga acacggacgg ctgctcgatg
     6721 caccgtttta cgcccgcttt ggtgttgagt tcgtcgatga agcccgcgtg ccaggcatca
     6781 agctgcgcgg cgtcggagaa tacctggatc tggaacgctt tcgcttgccg gcgctccagt
     6841 ttttgctccg ctacaagacc tatgttgcgc gcaggtccga cgacagccag gccgaataaa
     6901 aggccgcaaa cccacgcata tttttggggt ttcgcccgct tgccaacaag gcgatgatgt
     6961 cagggctcga tgcttccctc ggtaatccaa acaagccttg aggtttgggc tggcacttcg
     7021 ctgttcttct ctttgctcct gcttttcggc gtgctgcggc tccgaaaaac gccgcctggg
     7081 cccccgcccg cctggccagc gatcgtggtt ttgcgcccct gcgagggcga cgagccaggt
     7141 ctttacgaaa acctgttgtc gtcgtgtctt gcgccgtatg aagggccgcg tcgggtgctg
     7201 ctgttgtgtc cgagcacgtc cgatccgtcg tacccgctgc ttttgcgggt ggcaaaggcg
     7261 gcaccgccca ccattcccgt cgaagtacta ctgacgcggc ccgaggccct gcacaaccgc
     7321 aaggtgtcgc agctgctggt gggaattgcc cgatcgaccg agccgatctt gatcactgcc
     7381 gacagcgatg tcctgttggg agaaaaggat ttgccgtcgc tggtgtcgtt gctttgcgcg
     7441 ccgccaagca cgccggtgca gcgtcagcaa gtcggcattg cgttcgcagc ccccgtcgaa
     7501 caacagccgc ggacgcgctg ggatcggctg tcgtcggcac tggtgggtgg ctcggtgcaa
     7561 aacttcatgg cgctctacgg gctgtatcgg ctgatcggtg gggtgccgag catggcggga
     7621 gcgcttttgg cgatttcccg ccgcgcgctc ttagacatcg gcggtcttca ggaagtgcga
     7681 acgattctcg gcgaagacca cgaactggcg cgtcggctct gtgcggcggg ctattccgtc
     7741 gagcaaagcc cgtttccagc ccactgttcg gatggcgaac gatcgcttta tggggtgatt
     7801 tcacgggtgg cacgctggct gatggtggtg cgcgcacagc gacctctttt gcttctgacg
     7861 tatccgctgt ttatggcggc aacaccaccg ctgctcgtgg ccacagcgat cttgcggacc
     7921 ccgctgcttt cgctgctggc cggcctgatt ttcaccgccc gcacgctgct ttcgtggctt
     7981 ttgcgccgtg cccaacacgt ccagcagggg tttttcgaaa gcgcgctcga ggtgatcggg
     8041 gcggagctgc tgctgtggac gggacttgcc aaggcgctgg cgacccggca gataaactgg
     8101 cgcggctatc gctttcgcat cgcacgcggc gggcacatga ttcccgaagc gcaggccccg
     8161 tcggcgcagc cttaagcgac gcggcgggac caggcaagca gggtgttagg cgtttcgacc
     8221 caactgcgcg agcagctcgc tcacatcggg aggaatcgcg ccgcccgact gtttgtgcat
     8281 cagcagcagc ttgaggaccg cgcgggccgc ctcggcaacc agatccggtc cggcgctgtg
     8341 tccggcggaa acgggtggat gggccgccgc ctgcgctgcc gccggagcgt tcgaactggc
     8401 gacctgcgct ggtgcctggg cagtcgtcga tgctggggtt tccgtcgcgg cggcttcgct
     8461 tgactgcgga aacagcgtga gcacttccgc cgcatgcttc cacacgtcga cgcgcggaaa
     8521 catgtgggtg atgacgccct tggcattcac gataaacgtc atgcgcgcgg tgttgtcgtt
     8581 gcgcagcgcg ccccattcct cgctgatgcg gcgcatcgaa tcgcacagca cgtcgaagtt
     8641 gaggttgtgc tctttcgcaa acgcggtgat ttccgacgcc gggctggccg tgatgccgac
     8701 caccttgacg cgccgtttgg caagctccgg cacgatgtcg cggaagccga tcgcttcacg
     8761 cgcacagccg ggcgcccccg cctgcggaaa gaagtagacc acgacaaagt ctgcctcgga
     8821 ctgaagccgc ccgagctgca ccatgtgctg gtcctggttg tacaaagcaa agtccggtcc
     8881 gagtccaccc actgttggca tggttattta tattactaat ttttcacaaa atacacatgc
     8941 ccggcacagc ttcagcctgc gtcgaggact ggacgcctgc tggggaaaag cgcggcgccg
     9001 ccagggcagg cccctcgcgc ttgctggtga gatcacggtt gtttttggcc cagcgtgctt
     9061 tcgcggtttg acaatgcgaa aataggcacg gcgcttttct gacacagcga agggggagtt
     9121 tcgacggcaa ttgggatatg tggcagtcac caattgtcga cccttcacct acttgataac
     9181 ggggaacacg gtcttatttg tcaaaattgg tcgaaccaat agcaaccatc tggaggcatt
     9241 gagcatgaga aaacacgtac tcgctctgat gtccgttacc tacctattga tgggctgcaa
     9301 tgc
//
LOCUS       DAORGN010000196         9176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3312_length_9176_cov_4.878193, whole genome shotgun sequence.
ACCESSION   DAORGN010000196 DAORGN010000000
VERSION     DAORGN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_9176_cov_4.878193"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKL17_17510"
     CDS             <1..667
                     /locus_tag="PKL17_17510"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNK46584.1"
                     /translation="VEIVQQLGWQVPDWVVVPGGNLGHVTAIARGFAMAKTLGLIDRE
                     PRLLVAQAEQASPLYHAFCNRWQFVREVAGPTQATAIAIGNPVNVHKAIAELQRVDGA
                     VEAASEDEITTAWTLGDRHGLQTDPHTGVALAALAKQVEKGRILPSQRVVVVSTAHGL
                     KFGTLKRDFHLGEAAAQTAGPTSQATANKQARRNPPLRLPPHLPTLDDVVLRKLDDAE
                     RAR"
     gene            complement(632..3958)
                     /locus_tag="PKL17_17515"
     CDS             complement(632..3958)
                     /locus_tag="PKL17_17515"
                     /inference="COORDINATES: protein motif:HMM:NF039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HNK46585.1"
                     /translation="MKGDFTRFTFRPEKQYTSVRLQQGRVQLDADWNEQVDIHAYQMR
                     VMQSSLFGDGAPRASAGFKVGFDTSRGLTLSPGRYIVGGLVCELPASASDASVGATYK
                     DQPFFPSPPLLSGMAAGNYLAYLDVWTRHVTALEDPLLREVALSGPDTTTRTQTVWQL
                     RLLKLDTALGALDTIDLGAKIPASSIKMAARALALAPTAGSSLQPPGSGYLRLDNHLY
                     RVEIHQGGKLGTDPVTFKWSRDNGSVVSQLQGIVGNILQVGPVPLDEERGFVVGCWVS
                     LTDDSSELRNEPGIMCRVTAVEGRNITVQPPPGPALNLSGFGPNPKVRRWDMVTPSLQ
                     SVTTGGWIDLEDGLSVQFSGTTCASGDYWLIPARTATRDIEWPRSGSDPAFVPPAGIG
                     HAYDDIASFAWDGTTATSIIDRRAVFPSLSDMKLFDPVVYSQTMYGHTHTGTTDGSPI
                     PRGGIQDGAINGAKIATDSDVKISKLNVTGNLDLLSSSNVLHVANAWYGPWISETSSA
                     AICNDLGSFKALMLIGNRGGVANAPRKVGVWDALEVNGTLKTTSDTDVGGLLSIRGTD
                     LRIDNPSSRGGRDGSYRRALVHDSDDCLTINKNQDFSSGVKVSGQLSMDGRVILNENI
                     LRLRSAQSFNHGLGYFGANNTYAGVGIEGPVLFGRDGGGLGTTSVAANEVDGSLYLTG
                     GGYVSIPAFQEDFSGGVTVEAWVRYDSLPSWSRVFDFGGGQSSDNIILANDGTSSTVR
                     FNLHNGASGSATTSVYGTGALATGTWIHICATIDASGTGKLYKNGAQFGNTQTGLALP
                     TGVSRTSNFIGKSNWSNDAAFNGRIAHVRIWKRALSSDQVSDNYTSARPMDTTGLVAW
                     WRLDDGSGTTVRDASGKGRHGTFVPGSTSSGWDNYQKLALRWDNSNNVTVAGRLSIGG
                     SDVYLDCTERRDGATGSYRRALVHNSGDVLALNFASDYTGGVTIGGNTTVSGKLKSSN
                     TKTSTSVSSGTVWSTTASAYTDISDISVSNVTVTGGSCLVLLTLGGVQIEGNTNTRCF
                     FQILYQQSGASTWNSVAISVHEFSVSGYSLRDVQMHAILAPSAGTYNFKVQVYTNTTS
                     STIKIGYYSSTRTLSVIELA"
     gene            complement(3967..6102)
                     /locus_tag="PKL17_17520"
     CDS             complement(3967..6102)
                     /locus_tag="PKL17_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46586.1"
                     /translation="MSPALTKDLVRERLYALLPTIYRMRDFGQGEPLRALLLLLENEL
                     LRVESDIARLYDNWFVETCDEWAVPYIGDLIGSRLLHPVHARAFTANSLAHKRRKGTV
                     QTLGALARDITGWPAQAVEYMPLVVTSQHVNSPTARNRATTSLRCAQVLDNIGTPFET
                     TTRSVDVRSRGRFLQNQLGVYVWRADVTRRDMVATLPLSAGVYAVSPLGMPQSLWSPA
                     SIRAGLEAPMDLKLSPQPIRRRVLYQSLEARRQALIDGRTVLDPYFDPEPVLRIFYRD
                     SMGNVVAVLPEQMVAADLTTPWRPAPQLTYKRSGDGKQVQRRIVAAVDPELGRVAFAT
                     DVNPSKVWTTYYASSMGSIGGGGYERTLREVGNLPVVLLAGGGDQPALTALQDPAQWP
                     GGQVAGQSPSVVIEFGDSDIYTLPLVKVSDGGTLVLRARSQCCPTLLAGDSPLPIQLG
                     NGATLVLDGVHVRGGFVIQPLSGTAPGFSAQVTVRHATLVPGFMLDPAGLPRFPSTPS
                     LSVSNGFPPGSVSVSLEHAISGPIIADTFAVTACDSIIDAPLRASPAVVAGSVQFSRV
                     TVLGACLVTRVLAIKDSVLLDRLLVVDRDSSTFAVTYSVLPYDPTLGPVTYRCQPFLS
                     VNEAQTADEKRRLADMLRPQLSASRYGAPGYGQLSVRCATALRSAASDQGEPGAFHQL
                     QQALRESNLIDSQAEYLRSGLTLNLFVVT"
     gene            complement(6099..8849)
                     /locus_tag="PKL17_17525"
     CDS             complement(6099..8849)
                     /locus_tag="PKL17_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46587.1"
                     /translation="MSDSQDLGSDGSVNLPGQTTISYRATTHGQSLARLKAGLATVAI
                     PSGPNQGKMPLSALKTLAEDEPTVALLDAFSTVLDVVSFYSERIASEGYLRTALEDRS
                     RHWLAQQIGYRPQPALAASTYLAFDIDDTATMPTQVAIAAGSQVMSMPSSGSQTQTFE
                     TQDALTARVAWNALLPRLTQPQRVVATNQSTAGTAVSTLRFAGLATGLWVGSIVRIAD
                     ATTVQVTAVSRSNSEQVTDVSFQTLDGPTVRIATPDVLPAGDPKTLPQVLSKAVVSEH
                     ILGKSWDGDVLSDCIAARGFGESQLTSLVASVLSAQTGTQVLTYRQQASFYGSSAPPW
                     SSMMKISSDYLRGTDLYSATSASWDEATSSGTASTSNVKQYRNKRSIWEDSWGQLYSA
                     GSDYDVFLDRVVSGVAPGSVGLLACPRGYRSVTLVGVGATNVRGYGTTARVTGLDLSP
                     VPASLAAAQADTFLTRDSVMYLQSESLSLSTLIPRLDSAVVTDSVELSTFSLGLSRGQ
                     AVLVSGEHANQSGVSTSEVALLSQVVHRGGYTTLYFAKPLQNPRLRSSVSICANVVLA
                     THGKTVSGEVLGSGDAGQKNQRFQLAQGPLTYLSDDSESGLKSTLSIRVSGVLWHPVS
                     SLLAEDGRSRCYVVSHDADGHTTVTFGDGEHGARLPSGKDNVVATYRIGSGKDGEVSA
                     GGLSILLSRPLGLRGVTNPIAAAGAEAAASAADILEEAPLSVATLGRAVSLSDYEVMA
                     KLYPGIAKAYATAVRSAGAPAVHLTVASDDGQPLSASPTLRQGLMQTLLSLGDSTQPL
                     FLDDYRPRYFRVALGIAVDSDYLADSVVALVSAALAQRFSFAAGDFGNSVRAADVLSV
                     AQSVPGVLGAVLSGLWFDGSPQSKQLTLAAESARIDRGGLSCGAEILLILLDDSSVGV
                     MP"
     gene            complement(8846..>9176)
                     /locus_tag="PKL17_17530"
     CDS             complement(8846..>9176)
                     /locus_tag="PKL17_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNK46588.1"
                     /translation="VGVKSGHFRGDVVAALSAALGPGTASRPGFFHPDRQSLGQPVYL
                     SRLIRAAMDVKGVAWVECNRFRRYFSSQTTDDDVIVLRPTELASVENIPTQKSRGFLE
                     LVIGGKP"
BASE COUNT         1783 a         3063 c         2721 g         1609 t
ORIGIN      
        1 ggtggaaatc gtccagcagc ttggctggca ggtgcccgac tgggtggtgg tccctggcgg
       61 caacctcggg catgtgacgg cgattgcgcg cggctttgcg atggccaaaa cgctgggact
      121 gatcgatagg gaaccgcggc tgttggtcgc ccaagccgag caggcctcgc cgctttacca
      181 cgccttttgc aaccgctggc agtttgtccg cgaagtcgcc ggtccgaccc aagccaccgc
      241 gatagccatc ggcaacccgg tcaacgtcca caaagccatt gctgagctac agcgcgtaga
      301 cggtgccgtc gaagcggcca gtgaagacga gatcacgacg gcctggacgc tgggcgaccg
      361 tcacggactc cagaccgatc cacacactgg cgtggcgctc gccgccctgg ccaagcaagt
      421 cgaaaaagga cggatcttgc cgtcgcagcg cgtcgtggtc gtcagcaccg cacacggcct
      481 gaagtttgga acgctaaagc gcgactttca ccttggcgaa gccgccgcgc agaccgcagg
      541 gcccacgtcg caggcgacag cgaacaagca ggcgaggcgc aacccgccgc tgcgcctgcc
      601 gccgcatctg ccgacgctcg atgacgtggt gctacgcaag ctcgatgacg ctgagcgtgc
      661 gcgttgagct gtagtaccca atcttgatcg tcgaggacgt ggtgttggta tagacctgga
      721 ccttgaagtt gtacgtgccg gcagacgggg ccaaaatcgc atgcatctgc acatcgcgca
      781 gcgagtaacc actgacggaa aactcgtgga cgctgatcgc gaccgagttc caggttgaag
      841 cgccggactg ctggtaaaga atttggaaga agcagcgcgt gttggtgttg ccttcgatct
      901 gcacaccacc gagcgtcaaa agaaccaggc agctcccgcc cgtgacggtc acgttgctca
      961 ccgagatgtc gctgatgtcg gtgtaggcgc tggccgtggt gctccacacc gtgccgctcg
     1021 atacgcttgt gctcgtcttg gtgttgctgg acttgagctt gccgctgacc gtggtgttgc
     1081 cgccaatggt gacgccacct gtgtagtcgc tggcaaagtt tagcgccaac acgtcgcctg
     1141 agttgtgcac cagcgcccgt cggtagctgc cagtcgcccc atcgcgccgc tcggtgcagt
     1201 cgagatagac atcgctgcca ccaatcgaca gccgacctgc gaccgtgacg ttgttcgagt
     1261 tgtcccagcg cagcgccagc ttctgatagt tgtcccagcc ggaactggtc gatcccggaa
     1321 caaacgtgcc gtgtcgaccc ttgcccgacg catcgcgcac cgtcgtgccg ctgccgtcat
     1381 ccagacgcca ccaggcaaca agacccgtgg tgtccatggg ccgcgcgctc gtgtagttgt
     1441 cgctgacttg atccgatgag agcgcccgct tccagattcg cacatgggcg attcgtccgt
     1501 tgaacgcagc gtcgtttgac cagttgctct tgccgatgaa gttgctcgtg cggcttacgc
     1561 ccgtcggcaa ggccagtccc gtctgcgtat tgccaaactg agcgccattt ttgtacagct
     1621 tgccggtgcc acttgcgtcg atggtggcgc agatgtgaat ccaggtgccg gtcgcaagtg
     1681 cccccgtgcc atacaccgac gtggtcgcgc tgcccgaagc gccattgtgc aggttgaaac
     1741 gaaccgtgct gctcgtccca tcgttggcga ggatgatgtt gtcactggac tggccgccac
     1801 cgaagtcaaa cacccggctc caactcggca ggctgtcgta cctgacccac gcttcgacgg
     1861 tgacaccgcc ggaaaaatct tcctgaaagg ccgggatgct gacgtaaccg ccgccggtca
     1921 gatacagcga accatcgacc tcattggccg ccacactcgt ggtgccaaga ccgccgccgt
     1981 cccgtccaaa cagcaccgga ccttcgatac cgacgcccgc ataggtgttg tttgcgccaa
     2041 agtaacccag cccgtggttg aagctctgcg ccgaccgcag ccgcaggatg ttttcattta
     2101 aaatcacccg cccgtccatc gaaagctggc ctgagacttt gaccccgctc gaaaagtcct
     2161 ggtttttgtt gatggtcaag cagtcatcgc tgtcatgcac aagcgcgcga cgatagctgc
     2221 cgtcacgccc gccccggctc gatggattgt cgatgcgcag gtcggtgccg cggatgctca
     2281 aaagaccgcc gacgtccgta tccgacgtgg tcttgagcgt gccgttgact tccagcgcgt
     2341 cccacacccc aaccttgcgc ggggcgtttg cgacgccgcc acggttgccg atgagcatca
     2401 gcgctttgaa gctgcccagg tcgttgcaaa tcgcggcgct gctggtttcg gaaatccatg
     2461 gcccgtacca cgcattggca acgtgcagca cattcgacga agacaaaaga tccaggttgc
     2521 ccgtcacatt cagcttgctg atcttgacgt cggaatcggt ggcaatcttg gcgccgttta
     2581 tcgcgccgtc ctggatgcca ccacgcggaa tcggcgaacc atcggtggta cccgtatggg
     2641 tgtggccgta catcgtctgg gaatagacca ccggatcgaa cagcttcatg tccgacagcg
     2701 atggaaacac tgcgcgacgg tcgatgatgc tggtcgccgt tgtgccatcc cacgcaaacg
     2761 aagcgatgtc gtcataggcg tggccaattc ccgccggggg gacaaacgcc gggtcagacc
     2821 cgctgcgcgg ccactcgatg tcacgggtcg cggtgcgggc cggaatcagc cagtaatcgc
     2881 cactcgcgca cgtcgtgccc gaaaactgca ccgacagccc gtcttccaga tcaatccagc
     2941 cgccggtcgt cacactttgc aaagacggcg tcaccatgtc ccagcgccgc actttggggt
     3001 ttggaccaaa gccagacagg ttcagcgcag gaccgggcgg cggctgcacc gtgatgttac
     3061 ggccttcgac ggcagtgacc cggcacatga tgcccggttc gttgcgcagc tcgctgctat
     3121 cgtcggtcaa agacacccag cagccaacca caaagccgcg ctcctcatcg agcggcaccg
     3181 ggccgacctg caagatgttg ccgacaatcc cttgcagctg cgacaccacc gatccattgt
     3241 cgcggctcca cttgaacgtc accgggtcgg tgccaagttt cccgccttgg tgaatttcga
     3301 cgcgatacag gtggttgtcg agccgcaaat agcccgatcc cggcggctgg agcgagcttc
     3361 ccgccgtcgg cgccagtgcc agcgcccgcg ctgccatctt gatcgacgac gctggaatct
     3421 tggcgccaag atcaattgtg tcgagcgcgc cgagcgcagt atcgagcttg agcagtcgca
     3481 gctgccacac cgtctgcgtt cgcgtcgtgg tgtccggtcc cgaaagcgcc acttcacgca
     3541 gaagcggatc ttcgagcgcc gtcacatgcc gcgtccacac atccaggtaa gccaggtagt
     3601 tgcccgcggc cattccagat agaaggggtg gcgacggaaa aaacggctgg tctttgtacg
     3661 ttgcgccgac gctcgcgtcg gacgcgctgg cgggcagctc gcacacaaga ccgccgacga
     3721 tgtagcgccc aggtgacagc gtaaggccgc ggcttgtgtc aaagccgacc ttgaaaccgg
     3781 cgctggcgcg gggtgcgcca tcgccaaaca gcgacgactg catcacccgc atttggtagg
     3841 cgtggatgtc gacctgttca ttccagtcgg catcaagctg cacacgaccc tgctggagac
     3901 ggacgctggt gtactgcttc tcgggacgga acgtaaatcg ggtgaaatcg cctttcatgg
     3961 acatcctcag gtgacaacaa agaggttcaa ggtcagcccc gagcgcaggt actcagcctg
     4021 gctgtcaatc aggttgcttt cgcgtagggc ctgctgaagt tggtgaaatg cgcccggctc
     4081 cccttggtcg gaagcggcgc tgcgaagggc ggtcgcacat cggacactca gctgcccata
     4141 accgggcgcg ccgtaccgac tggcggaaag ctgcggacga agcatgtccg cgagccggcg
     4201 tttttcatcg gcagtctggg cttcgttgac actcaaaaac ggctggcagc gataagtaac
     4261 cgggcccagg gtggggtcat acggcagcac gctgtacgtg acggcaaatg tgctgctgtc
     4321 gcgatccacc accagcaatc ggtccagaag cacggaatct ttgatcgcca gcacgcgcgt
     4381 aaccaaacag gccccgagca ccgtgacgcg cgaaaactgg acagacccag ccacgacggc
     4441 agggctcgcc cgcagcggtg cgtcgatgat gctgtcacag gccgtcaccg cgaaggtgtc
     4501 cgcgatgatc ggcccactta tggcatgctc cagcgaaacg ctgacacttc ctggcggaaa
     4561 gccgtttgac acactcaacg atggcgtcga aggaaagcgc ggcaggcccg ccgggtcgag
     4621 cataaacccc ggaaccagcg tcgcgtgtcg caccgtcacc tgggcggaaa aacccggtgc
     4681 tgtgcccgac agcggctgga tcacaaaacc gccccggacg tgcacaccgt ccagaaccag
     4741 cgtcgccccg ttgccaagct ggatgggaag cggcgaatca ccggccaaga gcgtcggaca
     4801 acactggctg cgggcgcgca aaacgagcgt cccgccgtcg cttactttga ccagcgggag
     4861 cgtgtagatg tcgctgtcgc caaactcgat gacgacgctg ggactttggc ccgccacctg
     4921 tccgcctggc cactgcgcag ggtcttgcaa cgccgtcagc gcgggctgat ccccaccgcc
     4981 ggcgagcagc accaccggca ggtttccgac ttcacgcagc gtgcgttcat acccgccccc
     5041 accgatggat cccatcgagc tggcgtagta cgtcgtccac actttgctgg gatttacgtc
     5101 ggtggcgaaa gccactcggc caagctctgg gtcgacggcg gcgacaatcc ggcgctgcac
     5161 ctgtttgccg tcgcctgacc gcttgtacgt aagctgcggc gccggtcgcc atggcgtcgt
     5221 caaatccgcc gcgaccatct gttctggcag caccgccacc acgttgccca tgctgtcccg
     5281 gtagaaaatc cgcagcaccg gctcgggatc gaaatacggg tccaacacgg ttcgcccgtc
     5341 gataagcgcc tgtcgccggg cttcgagcga ctgatacagc acccgccggc gaatcggctg
     5401 cggcgacagc ttcagatcca tcggcgcttc cagcccagcc cgaatgctgg ccggcgacca
     5461 caagctctgc ggcatgccaa gcggactgac cgcatagacc cccgccgaga gcggcaacgt
     5521 cgccaccatg tcgcgccgcg ttacgtcggc ccgccagaca tacaccccca gctggttttg
     5581 cagaaagcgc ccgcggctgc ggacatcgac ggatcgggtc gtcgtctcaa acggcgtccc
     5641 gatgttgtcc agcacctggg cacagcgcag gcttgttgtc gcccggttgc gcgccgtcgg
     5701 gctgttgaca tgctgcgacg tgacgaccag cggcatgtac tcgaccgctt gcgccggcca
     5761 gcccgtgatg tcgcgcgcca acgcgcccag ggtctgcacc gttcctttgc gccgcttgtg
     5821 tgccagcgag ttggccgtaa aagcccgcgc atgcaccgga tgcagcagcc tggacccaat
     5881 caggtcgccg atatacggaa ccgcccactc gtcgcaggtc tcgacaaacc agttgtcgta
     5941 aagccgcgca atgtcggact cgacgcgcag cagctcgttt tccaaaagca ggagcagcgc
     6001 ccgcaacggc tcgccctgcc caaagtcgcg catccgataa atcgtcggca aaagcgcata
     6061 caaccgctcg cgcacaagat ccttggtaag cgccggactc atggcatcac ccccacgctg
     6121 ctgtcgtcga gcaagatgag cagaatttcc gccccgcagc ttagtccccc gcgatcgatc
     6181 cgcgccgatt ccgccgccag cgtaagctgc ttgctctgtg ggctgccgtc aaaccacagc
     6241 cccgaaagca ccgcgcccaa aacgccaggc accgactgcg ccacgctgag cacatccgca
     6301 gcacgcaccg aattgccaaa atcccccgca gcaaaggaaa accgctgcgc cagcgccgcg
     6361 ctgacgaggg caaccaccga atcggccaga taatcgctgt cgacggcaat tcccagtgcg
     6421 acccggaaat agcgcgggcg atagtcatcc aaaaacagcg gctgcgtcga atcacccaaa
     6481 gacaaaagcg tctgcataag cccctgtcgc agcgtcggag aagccgaaag cggctggcca
     6541 tcatcggacg ccaccgtcag gtgaaccgcc ggcgccccag ccgatcgcac cgccgtggca
     6601 taggctttcg cgatgccggg atacagcttg gccatcactt cgtaatcgga cagcgacacc
     6661 gcgcgtccga gcgttgccac cgatagcggc gcttcttcca aaatgtccgc agcactggcc
     6721 gccgcttcgg ccccggctgc cgcgatggga ttggtcacgc cgcgcagccc cagtgggcgg
     6781 gacaaaagga tgctcagacc gccggcggaa acttcgccat ctttgccact gccgattcga
     6841 tacgtggcaa ccacattgtc cttgcccgat ggaagccggg ccccatgttc accatcgcca
     6901 aacgtcaccg tggtgtggcc gtcggcatcg tgcgacacca cgtaacagcg gctgcgtccg
     6961 tcttctgcaa gcagcgaaga aaccgggtgc cacagcacgc cgcttacgcg aatgctcagt
     7021 gtgcttttga gccccgactc gctgtcgtcc gacagatagg taaggggccc ctgcgcaagc
     7081 tggaagcgct ggtttttctg cccagcatcg ccgctgccca ggacttcgcc cgacaccgtc
     7141 ttgccatgcg tcgccagcac cacgttggca cagatgctga ccgatgaacg aagccgcgga
     7201 ttctgcagcg gcttggcaaa gtacagcgtc gtgtaaccgc cccggtgaac cacctgggat
     7261 agcagcgcga cttcacttgt cgacacgccg ctctggtttg catgctcgcc gctgaccagc
     7321 accgcctgac cgcgcgaaag tccgagcgaa aacgtcgaca gttccaccga atcagtgaca
     7381 accgcgctgt cgagccgcgg aatcagcgtc gatagtgaca gcgactcgct ctgaagatac
     7441 atcacgctgt cgcgcgtcaa aaacgtgtcc gcctgcgccg ccgcaagact tgccggcacc
     7501 ggacttagat ccagacccgt cacccgcgcc gtcgtgccat agccgcggac gtttgtggcc
     7561 ccgacaccga ccagcgttac gctgcgatag ccacgcggac acgccaaaag acccacgctg
     7621 cccggtgcca cgccggacac cacgcggtcg aggaacacgt catagtcact gcccgcgctg
     7681 taaagctgcc cccacgaatc ttcccagatg ctccgcttgt tccggtactg cttgacgttg
     7741 cttgtcgatg cggtgccgct cgatgtcgct tcgtcccagc ttgcgctcgt ggcggaatac
     7801 agatccgtgc cgcgcaaata gtcggagctg attttcatca tgctgctcca cggcggtgca
     7861 ctgctgccgt aaaagctggc ttgctgccga tacgtcagaa cttgtgtgcc ggtttgcgca
     7921 gaaaggaccg acgccacaag acttgtgagc tgcgactcac caaagccgcg cgcagcgatg
     7981 cagtcagaca gcacatcacc gtcccaggat ttgcccaaaa tgtgctcgct gaccaccgcc
     8041 ttggacagca cctggggcag cgttttggga tctcctgcgg gcaggacgtc cggcgtcgca
     8101 atccgcaccg tcggcccatc cagcgtctga aaagacacgt ctgtcacttg ttcgctgttg
     8161 gaacgcgaca cggcggtcac ttgcaccgtc gttgcatccg cgatgcgcac gatcgatccg
     8221 acccaaagcc cggttgccag accagcaaag cgcagcgtcg acactgccgt tcccgccgtg
     8281 ctttggtttg tcgcaaccac gcgctgcggc tgggtcagtc tgggcaaaag cgcgttccaa
     8341 gcgacccgtg ccgtcagggc atcctgcgtc tcaaaggtct gggtctgcga cccgctcgac
     8401 ggcatgctca tcacttggga accggcggca atcgccacct gcgtaggcat cgtcgcggtg
     8461 tcatctatgt cgaacgccag ataggtactg gcggcgagcg ccggctgcgg gcgatagccg
     8521 atctgctggg ccagccagtg ccgcgagcgg tcttccagtg ccgtccgcag atagccctcg
     8581 ctcgcgatcc gctcggaata aaacgacacc acatcaagca cggtcgaaaa cgcatcgagc
     8641 agcgcgaccg tcggttcgtc ttccgccagc gtcttcagcg ccgacagcgg catctttccc
     8701 tggttgggcc cgctcggaat cgccactgtc gcaagacccg ctttgagccg cgccaggctc
     8761 tggccatggg tcgtcgcgcg ataagaaatc gtcgtctggc ccggcaggtt gaccgaacca
     8821 tccgagccca agtcctgcga gtcgctcatg gcttgcctcc aatcaccagt tccagaaagc
     8881 cgcggctttt ctgggtggga atgttctcca cgctcgccag ttccgtcggg cgcagcacga
     8941 tcacgtcgtc atcggtcgtt tgggacgaaa aatagcgacg gaagcgattg cattcgaccc
     9001 aagcgacgcc tttgacatcc atcgccgccc gaatcagccg cgacagatac accggctgcc
     9061 ccaacgactg ccggtctgga tggaaaaagc ccggcctact ggccgtcccc ggaccgagcg
     9121 ccgcgctgag cgccgcgacc acatcgcccc ggaagtgccc cgacttgacg cccacc
//
LOCUS       DAORGN010000197         9175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3313_length_9175_cov_6.030482, whole genome shotgun sequence.
ACCESSION   DAORGN010000197 DAORGN010000000
VERSION     DAORGN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3313_length_9175_cov_6.030482"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKL17_17535"
     CDS             <1..687
                     /locus_tag="PKL17_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46589.1"
                     /translation="LAGQDVTSGMSPYLHYGMLSAGEIARAVIAAHPQGLAHPPVAAY
                     IEELLVRRHLAHNFCLHAADPTQLAALPVWAQTSLREHGDDVRKVRYDRETLLRARTH
                     DPLWNAIQTELRELGEPHGYLRMLWGKRVIEWAPSYKEALSFLIECNDRFAYDGRDPN
                     GYTGILWCFGLHDRPFARRPVFGLLRPMSSDGAGKRAQGPVYIERVRRTLAGQSASGL
                     STQLGLRLGV"
     gene            complement(636..1547)
                     /locus_tag="PKL17_17540"
     CDS             complement(636..1547)
                     /locus_tag="PKL17_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46590.1"
                     /translation="MGFLLWAAPAQAQSTGIAAERMSFQSGPSAFYQVEGAQVAAPRQ
                     IWLHSGAIAVSHPLRIRNQASGELLSTPIDYRVTLDLGGELGLWKERLAVAVGIPVAV
                     WQNGDRLQSLGGSDLSVEKPLRVSAVGDLRLRARVRLTPTDWFWGILTTLDFTIPGGG
                     QDDFVATSGPTVAPKLVGWFQKGPVAFALNLSGRLAPHRQIYQTTIQHSVEWGAAASA
                     AFPTRRIGLALLAEAVGFYYPSAAIHSTELRGGLRFGLRQAAIDLGGGGGLGAFAPDW
                     RAFVSVRGYFNQTPSRSPSCVERPLAL"
     gene            complement(1701..2882)
                     /locus_tag="PKL17_17545"
     CDS             complement(1701..2882)
                     /locus_tag="PKL17_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46591.1"
                     /translation="MTTRSTLFYALVCCAIPAAVGLVSSCATVIVDPSYSSDGGTNPT
                     TGDGGNPNNSLSTFSAKVADTFEFPPETSLLAEAQRRWKRDPHYMTLNGNYYMFIAGS
                     DSSSLAERWSLSYYKDTAVPGARSPWTLLFEGPTSTSWDKYDLMAPFATYRQTDGWTL
                     YYAANGDPSKPDYVLQIGRATGADLSKLVRATQPVIGTPNFTGATPDTARPDAYGATD
                     PCVFVDGTDVYMYYAGLDCGSGVCKFQILRSKSPDGGSSFPPGDVVMSGRSGVAEEAG
                     GVAGPSIVKIGSQYVMAYTAVKDIPQKDRKSIRNALATGTVGMAVSADGKTFQSGTKT
                     GAALVPQLAGFSSFRSEGAFSPSLYLDGNVLRGWVSGLKNDANGTYFGIVSTELTEAK
                     P"
     gene            3245..4564
                     /locus_tag="PKL17_17550"
     CDS             3245..4564
                     /locus_tag="PKL17_17550"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNK46592.1"
                     /translation="MPERTKSLLEIKFYAATDIGRQRDHNEDNYLVDPRLSLFVVADG
                     MGGHAAGEVASQIAVHAVCKTVRDNADVIERQRAQADSAPARSEILGVLEHAIQSASA
                     EVFRRAQEDPDKRGMGTTCSCLLLVGNRGFFAHVGDSRIYLLRQNQVHQITEDHSLLN
                     ELVRRGKLKREEIEGSPYAKYKNAVTRAIGAYETVEADTFDFEVLPGDSFLLCSDGLH
                     AYLKDRDIKEILGGADIADSPKDLIALANSGGGHDNITAVVVRVESSDPGQSTELISE
                     LANKVSVLKGMPLFKHLTYKEVIRVLNLTTVLEVGAGETIFREREAGEELFVILSGRV
                     RLHKDNVALVVLGKGAHFGEMALVDRATRSATATAEEPTRLLALRRRDFYEILRKESP
                     LAVKLLWSFVQVLTERLRKTTAELSAGRAEQQTASSSSIPGEDEFQP"
     gene            complement(4611..5603)
                     /locus_tag="PKL17_17555"
     CDS             complement(4611..5603)
                     /locus_tag="PKL17_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46593.1"
                     /translation="MALRPLPVPPDPPFPSRSALRLVVLLWTLLLGIGKNHAQPATPQ
                     AQDVTPDTFSGVERIVAIGDIHGDHERFLATLKLCRIIDDKQSWIGGKTHLVQTGDIL
                     DRGPDSKKVIDLLMQLEEQAKKTGGKVHALIGNHEYMTIAGDLRYVSDGELAAFGDGP
                     RLTPVGREPACSVAKYRAAFGPQSKYGRWILSHNAIVKVNDTVFMHGGLSPRYLHRKL
                     NDLNRAVRTELQNPSPQSFGVGSDPEGPLWFRGLAESLQEQVQSAYLKELATAQGAGR
                     IVMGHTIQDKGISLRADGKIALIDVGMSRWTLGGAPSCLVIERSGNTDRLSILR"
     gene            complement(5618..6568)
                     /locus_tag="PKL17_17560"
     CDS             complement(5618..6568)
                     /locus_tag="PKL17_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46594.1"
                     /translation="MRRMAFGFAFLWLALAFCVRLYDLRKPQVYLSALTGGQKGMFAT
                     VAWLQGTLPWRVGPDKAHGRLMVVDASIEGKILRFSSLTLPKEWLDCRLWLEEGPDVL
                     QAMASCPGHAPLRLTASGQSLPGLGPDNRPAALDRVAPLTWVEPERIVSQVHRREAAP
                     SHPILLLLGLLAMAPLAVGAYRSYEQARRLRGKPILEGVMEETDKGSLTIRAGERRVT
                     VFVEEGEVLSIGLHGAAKSGDAMAVEGLRAAVSGDVEHQKDAAFRGGETMRLRRGAIL
                     VVGDLLSEARSRVLGTVAVDVALAGIGMILATVVALGWGF"
     gene            6670..8073
                     /gene="rlmM"
                     /locus_tag="PKL17_17565"
     CDS             6670..8073
                     /gene="rlmM"
                     /locus_tag="PKL17_17565"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: protein motif:HMM:NF008734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNK46595.1"
                     /translation="MLQRSAPSFSRPAPARREVPAKTATFPSPVTVGDWLWLCRQGAE
                     NDVCEELALYRLTAEMLAPALVRSAKKPPGDVLLTFARQGLPVEALLPPDAQAITAAM
                     SRRVKRPIVIHVFAPDSDDGNRLSSQAESLLSDLTTLLRAVGIDVLSDGEAAQQAGGH
                     LLQVCFLSADRVAVGLVAAVKAQSLFPGGRQRFRKPKDAPSRSARKLEEALAFSGHVP
                     AAGEVCVDLGAAPGGWSQVLLERRCHVVAIDPGRLAPAIAKRVEHLRMNAFSFAPEIP
                     ADWVVCDMAYRPLEVAALLARWGRHRWAQFLIANFKLPMKKRVEMVGRIREILETGGW
                     TGIKMRQLYHDREEVTVAAWRGFGRETWVGKRASEGEKTTENAQFSPKSPEFAPNRSQ
                     ATAKGPGRGKGQSAGKTGGRRQARGGQGSGGFGAKKTAKPERGKKAQPRESSRSGPKD
                     VRTSRVSGRSKGHVRRP"
     gene            8057..>9175
                     /gene="larE"
                     /locus_tag="PKL17_17570"
     CDS             8057..>9175
                     /gene="larE"
                     /locus_tag="PKL17_17570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNK46596.1"
                     /translation="MSVAPDLSVSTFASTPSEELLAKLALLRRVIAGYGPSLVAFSGG
                     VDSALVLKIAADELGDAAVAFTAVSETMAEREIDAAAKLAESLGVRYEAVRSNELARP
                     GFAQNPADRCYHCKSELFDLAEPARLRLGLAHVLLGTNLDDLGDHRPGLVAARERGAK
                     QPLVEAGLGKADVRTLARYLGLDVWNKPQLACLSSRFPYGTQLTEERLKMVDRFEQSL
                     HDLGFVQLRVRFHELPALPGDPLSTRPAMARVELLATSLADAIRRRAEIVEAGKRAGF
                     LYVTVDLEGFRSGSGNLVLRRLPLVGENPPALPVAPPPVATEKKTAVRPRKIVVAALV
                     IREPSGEVLLSLRRPDQAMPLLWELPGGKIEPGESPEEA"
BASE COUNT         1744 a         2794 c         2887 g         1750 t
ORIGIN      
        1 ctcgccgggc aggacgtcac gtcggggatg agtccctatc tgcactacgg catgctttcg
       61 gctggcgaga tcgctcgcgc cgtcatcgcc gcccacccgc aaggactggc ccatccgccc
      121 gtggccgctt acatcgaaga acttttggtc cgtcgccacc tggcgcacaa cttctgtctg
      181 catgcagccg atccgaccca gcttgcggcg ctgcccgtgt gggcacagac gagcctgcgt
      241 gagcatggcg acgatgtacg caaggtgcga tacgaccgtg agacgctgct ccgcgcgcgg
      301 acccacgacc cgctgtggaa tgccattcag acggagctgc gcgagctggg tgaaccgcat
      361 ggctatctgc ggatgctgtg gggcaagcgc gtcatcgagt gggcaccgtc gtacaaagaa
      421 gcgctgtcgt ttcttatcga gtgcaacgac cgctttgctt acgacgggcg cgatccgaac
      481 ggctacaccg gcattttgtg gtgttttggg ctccatgacc gaccgtttgc gcggcgtccg
      541 gtgtttgggc ttttgcgacc aatgtcttct gacggtgccg gcaagcgagc acaggggccg
      601 gtctatatcg aaagggtgcg gcgcacgctg gcgggtcaga gcgcgagcgg tctttcgaca
      661 cagcttgggc tgcggctcgg ggtctgattg aaatagccgc gcaccgacac aaacgcccgc
      721 cagtcggggg caaatgcacc aagcccgcca ccgccgccca ggtcaatggc ggcttgccgg
      781 agaccaaacc gcagaccgcc gcgaagctcg gtcgagtgaa tcgcggcgct tggatagtaa
      841 aaaccgaccg cttcggcaag cagcgcaaga ccgatgcgcc gggttggaaa cgctgcactt
      901 gccgcggcgc cccactcgac ggagtgctgg atcgtcgttt ggtagatttg ccgatgcggg
      961 gccagtcgac ccgataggtt gagcgcaaac gcgaccgggc ctttttgaaa ccaacccacc
     1021 agcttcggtg cgaccgtcgg gcccgatgtg gcgacgaagt cgtcctgacc cccgccggga
     1081 atcgtgaaat cgagcgtcgt caggatgccc caaaaccaat cggtcggcgt gagccggacg
     1141 cgcgcgcgca ggcgaagatc acccaccgca cttacccgca gcggtttttc gacggaaaga
     1201 tccgaacctc cgagcgattg cagacggtcg ccgttttgcc agacagcgac ggggattccg
     1261 accgcaacgg ccaggcgttc tttccaaagt cccagctctc cacctaggtc gagggtgacg
     1321 cgataatcga tgggggtact gagcagctcg ccgctggctt ggttgcgaat gcgcaaagga
     1381 tgtgacaccg cgatggcacc actgtgcagc cagatttgcc ttggcgctgc gacctgcgca
     1441 ccttcgactt ggtaaaacgc cgacgggcca ctttggaacg acatgcgctc ggcggcgatt
     1501 cccgtcgact gcgcctgcgc tggtgcggcc cagagcaaaa accccaagcc gaaggacgat
     1561 acgcggccaa aagcgcgcga cagaagcgat ggcaacacga cgggaaacat acacgtttgc
     1621 cgagcgtgcc ccaagtgggg ctgccgaaaa acgcagtcgc ttggcggggg aacctacggg
     1681 agaggacggg cgacttcggc ttacggcttg gcttcggtaa gttccgtgct gacgatgccg
     1741 aagtacgtgc cgttggcgtc gttcttcaga cccgacaccc agccgcgcag cacgtttcca
     1801 tccaggtaca ggctcggcga aaatgcgcct tcggagcgga acgacgaaaa tccggcgagc
     1861 tgtggaacca gtgccgcgcc ggtcttggtg ccgctctgga aggtcttgcc atctgcgctg
     1921 accgccattc cgacggtgcc ggtcgcgagc gcgttgcgaa tcgacttgcg gtccttttgc
     1981 ggaatatctt tcacggcggt gtacgccatc acgtactgac tgccgatttt gacgatgctg
     2041 ggaccggcca cgccgcctgc ttcctcggcg acgccgctac gtcccgacat caccacatcg
     2101 cctggcggga aagatgatcc accgtcgggg ctcttcgagc ggaggatttg gaacttgcag
     2161 acgccgctgc cgcagtcgag cccggcgtag tacatgtaca catcggttcc gtcgacaaac
     2221 acgcaaggat cggtcgctcc atacgcatcg ggacgagccg tatcgggcgt cgctccagtg
     2281 aagtttggcg tgccaatcac cggctgggtg gcgcgaacca gcttcgacag gtctgctccc
     2341 gtcgcacgcc cgatctgaag cacgtagtcc ggcttgctcg ggtcaccgtt ggcggcgtag
     2401 tacagcgtcc agccgtcggt ctgacgatac gtcgcaaacg gcgccatcag atcgtacttg
     2461 tcccacgacg tgctcgtcgg tccctcgaat agcagtgtcc acggagagcg cgcacccggc
     2521 accgccgtgt ctttgtagta cgacaaagac cagcgttccg cgagcgacga cgaatcgctg
     2581 ccggcaatga acatgtagta gttgccgttt agcgtcatgt agtgcggatc acgtttccag
     2641 cgacgctggg cttcggcaag caggctggtt tccgggggaa actcaaacgt gtcggcgact
     2701 ttcgcactga acgtcgacag actgttgttc gggttgccac cgtctccggt cgtggggttg
     2761 gttcctccgt ccgagctgta ggaggggtcg acgatcaccg tcgcacagct ggaaaccaag
     2821 ccaactgcgg cgggaatggc acagcagaca agtgcgtaga atagggtcga acgtgtagtc
     2881 atgaagaacc tcatcaagag catagcacgc ttttttccgt caccaaaccc ggtttcacaa
     2941 gcgctgcggc aggttagctc gggtcggggg tcgaactttg gtcgcgctcg cggtttacct
     3001 cgggctgcgg gttcgggaca tggcccatga agagcataga gccggggcag aattgcgctt
     3061 ttccacacaa gattcttcgc ttgtgaagag gggaaaagcc gtggtacgac ttaatagcct
     3121 ggctccgatc tgaaagcgcg caagccatgg gtagcactaa aaaattggcc tgcgcacagc
     3181 gacgaaagac tggcggccct cgcttacgac gtctagggca gaaaccaccg acgagcaaac
     3241 catcgtgcct gagcgtacca agagcctgtt agaaattaag ttctacgcag cgacggacat
     3301 tggacgtcag cgcgatcaca acgaggacaa ctacctcgtc gatccgcgcc tgtctttgtt
     3361 tgttgtggcc gatggcatgg gaggacacgc ggcgggcgaa gtggcgtcgc agattgccgt
     3421 ccatgccgtc tgcaagaccg tccgtgacaa cgccgatgtg attgaacgac agcgcgccca
     3481 ggctgattct gcgccggcac gctcggaaat cctcggcgta ctagaacacg cgattcagtc
     3541 ggccagcgcc gaagtgttcc gtcgtgccca agaagaccca gacaagcgag ggatgggaac
     3601 cacctgtagc tgtctgctcc tcgtcggaaa ccgcggtttt tttgcccacg tcggcgattc
     3661 gcgcatctat cttctgcgtc aaaatcaagt ccaccagatc accgaagatc actcgctgct
     3721 gaatgaactg gtgcgacggg gcaagctcaa gcgcgaggaa atcgaaggtt cgccctacgc
     3781 gaagtacaaa aatgccgtca cgcgcgccat cggtgcgtat gaaacggtcg aagccgatac
     3841 attcgatttt gaagtcctgc cgggcgacag ctttctgctc tgttccgacg gactgcatgc
     3901 ctacctcaag gatcgcgaca tcaaggagat cctgggtggg gccgacatcg ctgactcgcc
     3961 caaagacctg atcgcgctcg cgaactcagg cggcggacac gacaacatca cggcggtcgt
     4021 ggttcgcgtc gagtcgtcgg atcccggtca gtcgaccgaa ctaattagtg aactggcaaa
     4081 caaagtttcg gtgctcaagg gaatgccctt gttcaagcac ctgacgtaca aagaggtcat
     4141 tcgggttctg aacctcacga cggtgctcga agtcggggct ggcgaaacga ttttccgcga
     4201 gcgcgaggcc ggcgaagagc tgtttgtgat cctgagcggt cgagtgcgtc tgcacaaaga
     4261 caacgtggcg ctcgtcgtgc tgggcaaggg cgcgcacttt ggcgagatgg cactcgtcga
     4321 ccgggcgact cgatcagcca cagccacggc ggaagaaccc acccggctgc ttgctttgcg
     4381 aaggcgggac ttttacgaaa ttttgcgcaa agagtcgccg ctggcggtca agctgctgtg
     4441 gtcgtttgtg caagttctga cggaacggct gcgcaagaca acggctgagc tttccgccgg
     4501 tcgtgccgaa cagcagaccg cgagcagctc ctcgattcct ggcgaagacg agttccagcc
     4561 gtagcggcag ccacaaatcc ggcccccaaa agcgcttgcc gacgggctgg ctagcgcaga
     4621 atcgacaatc gatcggtgtt gccgctgcgt tcgatgacaa ggcacgacgg tgcgccaccg
     4681 agtgtccagc gcgacatccc aacgtcaatc agcgcaattt ttccgtcggc acgcagcgaa
     4741 atgcctttgt cctgaatcgt gtgtcccatc acgatccgtc ccgccccttg cgccgtcgcc
     4801 agttctttta gatatgcgct ttgcacttgc tcttgcagcg attcggccag gccgcggaac
     4861 cacagcggac cttcggggtc actgccgacg ccaaatgact gcggcgacgg attttgaagc
     4921 tctgtccgca cggcgcggtt gaggtcgttc agcttgcggt gaaggtagcg cgggcttaac
     4981 ccaccgtgca taaacacggt gtcgtttact ttgacgatcg cgttgtggga caaaatccag
     5041 cgtccgtatt tgctctgcgg gccaaatgcg gcgcggtact tggcgacgct acaggcgggc
     5101 tctcgaccca cgggggtcag acggggacca tcgccaaacg ccgcgagttc accatctgac
     5161 acatagcgca ggtcgcccgc gatggtcatg tattcgtggt tgccgatcag cgcatggact
     5221 ttgccgccgg tttttttggc ctgctcttcg agctgcatca gcaagtcgat gacttttttc
     5281 gaatcggggc ctcgatccag aatgtctccg gtctgaacca ggtgcgtctt gccgccgatc
     5341 cagctctgtt tgtcgtcgat gattcggcac agcttgagcg tcgccaaaaa gcgttcgtga
     5401 tcgccgtgga tgtcaccgat ggcgacaatg cgctcgacgc cggaaaaggt gtccggggtg
     5461 acgtcctggg cctgtggcgt ggccggctgg gcgtggtttt ttccaatccc aagcagcagc
     5521 gtccacagca ggacgaccag ccgaagcgcg gaacgggaag ggaatggggg atcaggcggg
     5581 acgggcaggg gtcgcaaggc cataaagagc gtctttctta gaatccccag ccgagggcca
     5641 cgacggtggc gaggatcatg ccgatccctg cgagtgcaac gtcgacggcc acggtgccaa
     5701 ggacgcggct gcgggcttcg ctgagcaaat caccgacgac aaggattgcg cctctgcgca
     5761 gccgcatcgt ctcgccaccc cggaacgcgg catctttttg gtgttcgacg tcgcccgata
     5821 ccgcggcgcg cagaccttcg acggccatgg catcgccgga ttttgccgca ccgtggagac
     5881 cgatcgacaa cacttcgcct tcctcgacga agaccgtgac gcgacgctcg ccagcgcgaa
     5941 tggtcagcga gcctttgtcg gtttcctcca tcacgccttc gaggatcggc ttgcctcgca
     6001 gtcgacgtgc ttgctcatag ctgcgatacg ctccgacggc aagcggcgcc atcgcgagca
     6061 gaccgagcaa cagcaaaatc gggtggctcg gtgcggcttc tcgccggtga acctggctga
     6121 cgatccgctc tggttccacc caggtcagcg gcgcgacccg atccagtgcc gccgggcggt
     6181 tgtcggggcc aagtcctggc aggctctgtc ccgatgctgt caggcgcagc ggcgcgtgac
     6241 ccggacagct tgccatcgct tgcagcacat ccgggccttc ttcgagccac agacgacaat
     6301 caagccactc tttgggcagc gtcaggctcg aaaagcgcag gatttttcct tcgatactgg
     6361 cgtcgaccac catcaatcga ccgtgcgctt tgtccggacc aactcgccac ggcagcgtgc
     6421 cttgcagcca tgccaccgtc gcgaacatgc ccttctgtcc accggtcagc gccgacagat
     6481 agacctgagg cttgcgcaga tcatagagcc gcacacagaa cgccagtgcc agccatagaa
     6541 aggcgaagcc aaatgccatc cgacgcattg agtgtcctcc tccgggctta gtttttctgt
     6601 gatgccgtgg tctgcgcaag ggcttccttg cgcgaccttt tctactatgc tgccgccaat
     6661 cgaggctcca tgctacagcg ctccgctccg tctttttctc gtcctgcccc tgctcgtcgc
     6721 gaagtgcccg ccaagacggc cacttttccg tcacctgtca ccgttgggga ttggctttgg
     6781 ctatgtcggc aaggcgccga aaacgatgtc tgtgaagagc tggcgctgta ccgattgacc
     6841 gctgagatgc tcgcgccggc actggttcgt tctgccaaaa aaccgccggg cgatgtgctt
     6901 ttgacctttg cgcgccaagg acttcccgtc gaagcactgc tcccgccgga cgcccaagcg
     6961 atcaccgcgg cgatgagccg tcgggtaaaa cgtccgatcg tcattcatgt ctttgcgccc
     7021 gattctgacg atggcaaccg gctgtcgagc caagctgaga gccttttgtc cgatctgacg
     7081 acgctgcttc gtgcggtcgg aatcgacgtt ctttccgacg gagaagccgc gcaacaagcc
     7141 gggggccatc tgctgcaagt ctgttttctg tccgccgacc gagttgcggt ggggctcgtc
     7201 gctgcggtga aagcccagag cctgtttccg ggcggacgac agcgctttcg caagcccaaa
     7261 gatgctccgt cgcgatcggc gcgcaagctc gaagaagcgc tggccttcag cggccatgtt
     7321 cctgccgcag gggaagtctg tgtcgacctc ggggcggcgc cgggcggctg gagccaggtg
     7381 cttctggagc gtcgctgcca cgtcgtcgcg attgatcccg gtcgactcgc ccccgcgatc
     7441 gccaaacggg tcgagcatct gcggatgaat gcgttcagtt ttgcgccgga aattcccgcc
     7501 gattgggtgg tctgcgacat ggcctacagg ccgctggaag tcgctgcgct tttggcgcgt
     7561 tggggacgcc acaggtgggc gcagtttttg atcgccaact tcaagctgcc gatgaaaaag
     7621 cgcgtcgaaa tggtcgggcg gattcgcgaa atccttgaga cggggggctg gactggcatc
     7681 aagatgcggc agctctatca cgatcgcgaa gaagtcaccg tcgcggcttg gcgcggattc
     7741 gggcgcgaga catgggtcgg aaagcgagcc agcgaaggcg aaaaaacgac cgaaaacgcc
     7801 caattttcgc caaaatcgcc cgaatttgcg ccaaatcgca gccaggctac tgcaaagggt
     7861 ccgggccgtg gcaagggcca gtccgcgggc aagacaggcg gacggcgaca agcgcgtggt
     7921 ggtcaggggt cgggcggatt cggggccaaa aaaaccgcca agccagagcg tggaaaaaaa
     7981 gcccagcctc gcgaaagcag ccgaagtggg ccgaaggacg tgcgcacaag ccgcgtatcg
     8041 ggtagatcca agggccatgt ccgtcgcccc tgatctttcg gtttcgactt ttgcttctac
     8101 tccgtcagaa gaacttttgg ccaagctcgc cctgctcagg cgggtgattg ctgggtatgg
     8161 tccgtcgctg gtcgcttttt ccggcggcgt cgattcggcg ctggttctga aaatcgcggc
     8221 ggatgaactg ggcgatgctg ccgtcgcgtt taccgcggta tcggaaacga tggccgagcg
     8281 cgagatcgat gcggcagcca agctggctga gtcgctgggt gttcgctacg aagctgtgcg
     8341 ctcgaatgag ctggcgcgac ctggctttgc gcaaaatccc gccgatcgct gctaccactg
     8401 caaatcggaa ctgttcgatc tggccgagcc ggcccggctg cggctggggc ttgcgcacgt
     8461 tctgctcggg acaaacctcg acgatctggg cgatcaccgg cccggactcg tcgcggcgcg
     8521 tgaacgcggt gccaagcagc cgcttgtcga ggcggggctt ggcaaagcgg acgtgcggac
     8581 gctggcgcgc tacctcgggc tcgacgtttg gaacaagccg cagcttgcct gtctgtcgtc
     8641 gcgctttccc tacggcaccc agctcaccga ggagcggctc aagatggtcg atcgcttcga
     8701 gcagtcgctc cacgacctgg ggtttgtgca gcttcgcgtg cgttttcacg agctgccagc
     8761 gctgccgggc gatccgcttt cgacccggcc cgcgatggcg cgtgtggaac ttctggccac
     8821 ttcgctcgcc gatgcgatcc gccgacgagc cgagattgtc gaagccggca agcgggccgg
     8881 ttttttgtac gtcaccgtcg atctggaagg ctttcgttcg ggatcgggaa atctggtcct
     8941 taggcggctg cctctcgtcg gagaaaatcc cccggcgctg cctgtcgccc cgccccctgt
     9001 ggcgaccgaa aaaaaaacag cggttcgtcc gcgcaagatc gtcgtcgctg cgctggtgat
     9061 tcgggagccg tcgggcgaag ttctgctcag tctgcgcagg cccgatcagg cgatgccgct
     9121 gctgtgggag cttcccggcg gcaagatcga gccgggggag tcccccgaag aggcg
//
LOCUS       DAORGN010000198         9163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3318_length_9163_cov_6.360123, whole genome shotgun sequence.
ACCESSION   DAORGN010000198 DAORGN010000000
VERSION     DAORGN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_9163_cov_6.360123"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1073)
                     /gene="panC"
                     /locus_tag="PKL17_17575"
     CDS             complement(228..1073)
                     /gene="panC"
                     /locus_tag="PKL17_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNK46597.1"
                     /translation="MISPSPKLCETVASLRSTLKNRGRIGFVPTMGNLHEGHIQLMRQ
                     AQQLSDVVVASVFVNRLQFAPHEDFDRYPRTLANDMQLMAEAGVDVVFAPSEAELYPE
                     PQTCLVDPGPIATGLEGEFRPGFFRGVATVVSKLFHIVEPDVAVFGKKDYQQIQVIRH
                     MVRQLQLRVNIIAGETARAHDGLALSSRNGYLSEREREEAPRLYRTLRTCAQGLRDRS
                     ASIENLELHAVANLRQNRWRPDYVRICDQTTLAPATDATRHLVILAAAHLGQTRLIDN
                     LEVDR"
     gene            complement(1070..1825)
                     /locus_tag="PKL17_17580"
     CDS             complement(1070..1825)
                     /locus_tag="PKL17_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNK46598.1"
                     /translation="MGACRLDERLVSDGLCESRQRAQAMVMAGAVLVDGQVVVKAGQK
                     VAAAAQVQLKGDPLPFVSRGGLKLQHALAYFSLDVTGRRAIDIGASTGGFTDCLLQAG
                     AAAVIAVDVGYGQLAYKLQQDPRVTVLDRQNARHLLPEQLPYVPDLATCDASFISQTL
                     LLPAMVQLVAPESPILTLVKPQFEVEKGQVGKGGVVRDKALHEQSIARCMRAAEALGC
                     RVMGVSPSPIRGPAGNEEFVLYLVTGPRKGSPA"
     gene            1948..3780
                     /gene="aspS"
                     /locus_tag="PKL17_17585"
     CDS             1948..3780
                     /gene="aspS"
                     /locus_tag="PKL17_17585"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNK46599.1"
                     /translation="MTNFLATYARTHYAGELRQSHVGTQVVLLGWVQGRRDLGGRIFI
                     DLRDRSGISQVVFGPDIGKDAHLEADALRSEFCIGIVGTVKLRSDSGGQANPNLPTGE
                     IEVECSVLHIFSRADTPPFQIEDDVETREEIRLKYRYLDLRRPKLQKNFQLRSKLYRA
                     TRDYFHNNGFWEFETPFMVKYTPGGARNFLVPSRLNAGKFYALAESPQIFKQLFMVAG
                     MDRYFQIVRCFRDEDLRQDRQPEFTQIDVEMSFVTEEGIQTMMEGLVAKIWQETLGVE
                     IPRPFLRMPYKEAMALYGEDKPDLRNPLRLHDITDLVKAHDGGGVPLFKSTVDGGGIV
                     KLLRIPGEHAQKISGTEARKFEDLVKGLGAKGLGSARLEATAESGIAAAWTQSPFAKT
                     LSNELREAIAKDIGATPGDLLLFQFGRAKLCNTVLASLRQQMGKRLELIGSGWRFLWV
                     TDFPMFELAEGDKWVAAHHPFTSPKPEHVELLGKDNGAVLARAYDLVLNGNEIAGGSI
                     RIHQKDVQAKVFAALGLTEDDFRAKFGFLLDAFRYGPPPHGGIAFGVDRLAMLLTGAE
                     SLRDVIAFPKTQNGTDLMSDCPTEVSNKQLEDLFIRLAPRAPSA"
     gene            complement(3836..6286)
                     /gene="lon"
                     /locus_tag="PKL17_17590"
     CDS             complement(3836..6286)
                     /gene="lon"
                     /locus_tag="PKL17_17590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNK46600.1"
                     /translation="MANSNKTDGGEGRRRSSRVLPLLPLRDIVVFPSMVVPLSVGREK
                     SIQALEQAVAKDKLIVMCAQKRARTQDPESSDIYSIGTLGEVIQLLRLPDGTVKVLLE
                     GKKRVRVRRFVGGDSFLAVEIDDIVEPEEIGPDVMVLMEKVQEAFSNYIKLSRRIPQE
                     IVGSTETIEEPGRLADTVIPALQLKLVDKQMLLEIESPLARLTKLLELLSAELEVLQV
                     EKKIRSRVKKQMNRSQKDLYLNEQMQAIQKELGERDEFKNELTEIEEKIRQKKMSREG
                     QLKLRKELRKLKLMSPMSAEATVVRNYIDWVLSLPWDERTQDKLNIKEAEAILTEDHY
                     GLQKVKERIIEYLAVQALVKKLKGPILCLVGPPGVGKTSLAKSIARATGRRFVRLSLG
                     GVRDEAEIRGHRRTYIGAMPGKIIQSLKKAGSNNPVMLLDEVDKMSTDFRGDPSAALL
                     EVLDPEQNAAFVDHYLDLDYDLSDVMFIATANNLHSIPLPLQDRMEIIHLPGYTEWEK
                     LAIAQQYLIPKERELNGISNVQMDVTADTVRAVIHHYTKEAGVRGLEREVSTICRKVA
                     REVVAKVHTGRTPESLRFRITGQNLQKYLGVPRYRSSKMEERDEVGLSNGLAVTMFGG
                     ELLATEVSVVPGKGKLVLTGKLGEVMQESAQAAMSYVRSRADALGLERDFYQKVDVHV
                     HFPEGAVPKDGPSAGITMVTSLVSALLRLPVRRDVAMTGEVTLRGRVLPIGGVKDKIL
                     AAHRAGIFTCIIPSENQKDLKEIPPKVLRSMTIIPAEHMDEVLRSALVLTDPAKFLSE
                     PSQAVDWRTAHPTPESVQ"
     gene            6449..8503
                     /locus_tag="PKL17_17595"
     CDS             6449..8503
                     /locus_tag="PKL17_17595"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46601.1"
                     /translation="MSGRDKNIVGEVLAKTYRIERFLGAGSVGTVYVARHVRSGGLYA
                     VKVLHRRLATSADVYQRFQDEARLIATLRHPHILPITDFDRDESGVPFFVMDLLEGES
                     LQQRLKHKETLPFAQTMEMISQVGSALHAAHRSGIVHRNLRPENIFLVRHDLGDRVVE
                     TTKVTDFGLACFRRTPGSSRDVPPTVYNAPELLQEGASVDGRADQWALAALAYRVLSG
                     KSPFEEGSAEEIVERVTSEAPRPLGKLMPELPPHVVAAIHRGMARRREDRYETTLDFV
                     RAVTQKSSTGGHVDAVPENLVARASPPSGRVVAPPPPPAPPPPEIKAPAAPVPAPAPA
                     PVKPPVRPVSGSRPPSVPIPALAAPPPLFEKTPPPMPVPRQTSQLPVILGGGAVVVVL
                     LALIIVVAVRKPAQQQPQQQPVVQQPAQVVQPVVQPIVQDLAVPVVVAQNPDAASNHP
                     TPVVRDVGLQLRGAELPSDSAAAAVGAVAPAKPAPTPQGAAVSPPGPIGTASNVGLNP
                     NAVKPAGTQPANPTATNPTGVATNKPSGTATATPAAGGGDPSRAPATGNPPANPTSPM
                     TTPAAGGSDSATAETKPSEPPKPAGPSPEEVLADAKKTYLSGDKERAVQMALQAAEKP
                     GPHVIDAWRWVGGAACSIHDASIASRAYAHLGSQEHKKLLTELCERSGLVLQNGQFIN
                     VE"
     gene            8558..>9163
                     /locus_tag="PKL17_17600"
     CDS             8558..>9163
                     /locus_tag="PKL17_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNK46602.1"
                     /translation="MALSLDLIEKLPKTDLHCHLDGSLRLQTILDLAKKQGVRLPADT
                     PEGLMPYVFAGMGHRSLEDYLKAFDITLAVMQTEEALYRVAYELAEDCHRENVWYLEV
                     RYSPMLHTRGGLSLTQIVESVLQGLRAAERDFGIRTGVILCGIRSSSADSSLRMAELC
                     VAFKNRGIVAFDLAGAELNYPAKAHKQAFQLILDNNVNCTAH"
BASE COUNT         1703 a         2877 c         2809 g         1774 t
ORIGIN      
        1 gccgaccggg aagccgtaag cccagtccct gggccttctc gagtaggcga ttggaggccg
       61 gctggccctt ccagccactc ccgacagttc gttgcggcct tcttccgggc tcccgcacac
      121 tcccgacagt tcgttgcggc cttcttccgg gctcccgctc actcccgaca gttcgttgcg
      181 gccttcttcc gggctcccgc tcactcccga cagttggttt tggggggtta gcggtcgact
      241 tctaggttgt cgatgagccg ggtttggcct aggtgggcgg cggcgaggat gacgaggtgg
      301 cgggtggcgt ctgtggccgg ggctagcgtc gtctggtcgc agatccgcac gtagtcgggg
      361 cgccagcgat tttggcggag gttggcgacg gcgtgcagct cgaggttttc gatgctggcg
      421 ctgcggtcgc gcagaccctg ggcgcaggtg cggagcgtgc ggtacaggcg cggggcttct
      481 tcgcgctcgc gctcggacag gtagccattg cgggacgaca gggcgaggcc gtcgtgggcc
      541 ctggcggttt cgccggcgat gatgttcacg cgcagctgaa gctggcggac catgtggcgg
      601 atgacctgga tctgctggta gtcctttttg ccaaagacag cgacgtcggg ttcgacgatg
      661 tggaacagct tgctgaccac ggtggcgacg ccgcggaaaa agccggggcg gaactcgcct
      721 tcgagcccgg tggcgatggg gccggggtcg accaggcagg tctggggttc ggggtatagc
      781 tcggcttcgc tgggcgcaaa cacgacgtcg acgccggctt cggccatcag ttgcatgtca
      841 tttgccagcg tgcgcgggta gcgatcgaag tcttcgtggg gagcaaactg caagcggttt
      901 acaaacacgc tggccacgac gacatcggaa agctgctggg cttgccgcat gagctggatg
      961 tggccttcat gcaggttgcc catggtcggg acaaagccaa tccgcccgcg gtttttgagt
     1021 gtgctgcgca ggctggctac cgtctcacag agcttgggac ttgggcttat catgcggggc
     1081 tcccttttcg cggtccggtg acaaggtaca gcacaaactc ttcgttgcct gccgggccgc
     1141 gaatcgggga cggcgaaaca cccatcaccc ggcagcccag cgcttccgcc gcccgcatgc
     1201 agcgcgcaat cgactgctcg tgcagcgcct tgtcacggac gacgccgcct ttgccgactt
     1261 ggcctttttc gacctcgaac tgcggcttga cgagggtcag aatcggactt tccggggcga
     1321 caagctgcac catcgccggc aaaagcagcg tctgcgagat aaacgacgca tcgcaggttg
     1381 ccaagtcggg cacataggga agctgctcgg gcagaagatg ccgcgcgttt tgccggtcga
     1441 gcacggtcac tcgcgggtct tgctggagct tgtaggcaag ctggccatag ccgacatcga
     1501 ctgcgatgac cgccgcggct ccggcttgca aaagacagtc ggtaaaaccg cccgtcgatg
     1561 cgccaatgtc aatcgcccgc cggcccgtca catccagcga aaaatacgcc agcgcatgct
     1621 ggagcttgag cccgccgcgc gacacaaacg gcagcggatc gcccttgagc tgcacctgcg
     1681 ccgccgccgc cactttctgt cccgctttga cgacgacctg cccatcgacc agcaccgcgc
     1741 cggccatcac catcgcctgc gcccgctgcc gactctcgca gagcccatca gacaccaacc
     1801 gttcatccag ccgacaagca cccagttgcg acaaggcaaa agtcctccaa atcaccgcga
     1861 tgaataccaa agccgcctgc ggaacgaaaa aaagtctcgc agggaagggg ggccccgctc
     1921 cgtgatcctt gctaaactgc cgccaccatg acgaattttc tagccaccta cgcacggact
     1981 cactatgccg gcgagctgcg ccaaagccac gtcggaaccc aggtcgttct cctcggctgg
     2041 gtccaaggcc gtcgcgacct cggcggacgc atcttcatcg acctccgtga tcgctcgggc
     2101 ataagccaag tcgtcttcgg gcccgacatc ggcaaagacg cccacctaga agccgacgcc
     2161 ctgcgcagcg agttttgcat cggcatcgtc ggcaccgtca agcttcgcag cgattcaggc
     2221 ggccaagcca acccgaacct cccgaccggc gaaatcgaag tcgagtgctc ggttctgcac
     2281 atcttttcgc gcgccgacac cccgccgttc cagatcgagg acgatgtcga gacccgcgaa
     2341 gaaattcgtc tgaagtatcg ctatctggac ctgcggcggc ccaagctgca aaaaaacttc
     2401 cagcttcgca gcaagctgta ccgcgccacc cgcgactact tccacaacaa cggcttttgg
     2461 gaattcgaga cgccgtttat ggtcaagtac acgcccggcg gcgcccgcaa cttcctggtg
     2521 ccgtcgcggc tcaacgccgg caagttctat gcgctcgccg agtcgccgca gatttttaag
     2581 cagctgttca tggtcgcggg catggatcgt tatttccaga tcgtgcgctg cttccgcgac
     2641 gaagacctgc gccaggatcg ccagcccgaa tttacccaaa tcgacgtcga gatgtcgttt
     2701 gtgacggaag aaggcatcca gacgatgatg gaagggctcg tcgccaagat ttggcaagaa
     2761 acgctcggcg tcgagatccc gcgtccgttt ttgcgcatgc cctacaaaga ggcgatggcg
     2821 ctgtacggtg aagacaagcc ggacctgcga aacccgctgc ggctgcacga catcaccgat
     2881 ctggtcaaag cccacgacgg cggcggcgtt ccgctgttca agagcacggt cgatggcggc
     2941 ggcattgtga agcttttgcg catccccggc gagcacgccc agaaaatctc gggtaccgaa
     3001 gcgcgcaagt tcgaggatct ggtcaaaggt cttggcgcca agggactggg atcggcgcgc
     3061 ctggaagcca cggcagaaag cgggatcgca gccgcatgga cgcagtcgcc gtttgcgaaa
     3121 acgctgtcga acgagctgcg tgaagccatt gccaaagaca tcggcgcgac accgggcgac
     3181 ctgcttttgt tccagtttgg ccgggccaag ctgtgcaaca cggtgctggc ctctctgcga
     3241 cagcaaatgg gcaaacggct ggagcttatc ggttcgggct ggcgcttttt gtgggtcaca
     3301 gacttcccga tgttcgagct ggcggaaggc gacaagtggg tcgccgcgca ccacccgttt
     3361 acgtcgccca agcccgaaca tgtcgagctt ttgggcaaag acaacggcgc ggtgctggcg
     3421 cgggcatatg acctggtgct aaacggcaac gagatcgcgg gcggatcgat tcggattcac
     3481 caaaaagacg tccaggccaa ggtgtttgcg gcgctcggac tcacggaaga tgacttccgc
     3541 gccaagtttg gctttttgct ggatgcgttc cgctatggtc caccgccgca cggtggaatc
     3601 gcgtttggtg tcgaccggct ggcgatgctg ctgacggggg cggaatctct gcgggacgtg
     3661 attgcgttcc ccaagacgca gaatggcacg gacctgatga gcgactgccc gacggaagtg
     3721 tcgaacaagc agctcgaaga tttgttcatc cggctcgccc cgcgggcccc ctcggcgtag
     3781 cggtccgaaa aaagctctcc ggtcggcccc acggctaggg ggctttttgg ggcaattact
     3841 ggacgctttc gggggtgggg tgggcggtgc gccagtcgac ggcttgcgag ggttccgaaa
     3901 ggaacttggc ggggtcggtg aggacaaggg cgcttcgcag cacttcgtcc atgtgctcgg
     3961 cggggatgat ggtcatcgag cgcagaacct tgggcggaat ctctttgaga tccttttggt
     4021 tttcgctcgg gatgatgcag gtgaaaatgc cggctcggtg ggcggcgagg atcttgtcct
     4081 tgacgccgcc gatgggcagg acgcgaccgc gcagggtgac ttcgccggtc atggcgacat
     4141 cgcgacggac cggcagtcgc aacagcgcgg aaacgaggct ggtcaccatg gtgatgccgg
     4201 cagacggtcc gtctttgggc acggcgcctt cggggaagtg aacgtggaca tcgacttttt
     4261 ggtaaaagtc gcgctccagg cccaaggcgt cggcgcggga acgaacgtag ctcatcgccg
     4321 cttgggccga ttcctgcatc acttcgccga gcttgccggt cagcacgagc ttgcctttgc
     4381 cgggcacgac gctgacttcg gtggccaaca gctcgccgcc gaacatcgtc accgcgaggc
     4441 cgtttgacag gccgacttcg tcgcgttctt ccattttcga cgagcgatag cgcggtacac
     4501 cgaggtactt ttgcaagttc tgcccggtga tgcgaaaacg caggctctcg ggcgtccgtc
     4561 cggtgtggac tttggcgacg acttcgcgcg cgactttgcg gcagatcgtc gacacttcgc
     4621 gttccaatcc gcgcacgccg gcttctttgg tgtagtggtg aatcaccgcc cgcaccgtgt
     4681 ctgccgtcac gtccatctgc acgttgctga ttccgtttag ctcgcgctct ttgggaatca
     4741 ggtactgctg cgcaatcgcc agcttttccc attcggtgta gccgggcagg tggatgattt
     4801 ccatccggtc ctgaagcggc agcggaatcg aatggaggtt gttcgccgtc gcgataaaca
     4861 tcacatccga taggtcatag tccaagtcga ggtagtgatc gacaaacgcc gcgttctgtt
     4921 ccggatcgag cacttcaagc agcgccgccg acggatcgcc gcgaaaatcg gtcgacattt
     4981 tgtcgacttc atcgagcagc atgaccgggt tgttgctgcc ggctttcttg aggctctgga
     5041 tgatcttgcc cggcatcgca ccgatgtacg tgcgccgatg gccgcgaatt tccgcttcgt
     5101 cgcgcacgcc accgagcgac agccgcacaa aacgccgtcc ggtcgcccgc gcaatcgact
     5161 ttgccagcga cgtcttgccg acgcccggcg gaccgaccag acacaggatc ggacctttga
     5221 gctttttgac gagcgcctgc acggccagat actcgatgat gcgctctttg accttttgca
     5281 gaccgtagtg gtcctcggta agaatcgctt cggcttcctt gatgttgagc ttgtcctgcg
     5341 tccgttcgtc ccacggcagc gacagcaccc agtcgatgta gttgcgcacc accgtcgctt
     5401 cggcgctcat cgggctcatc agcttgagct tgcgcagctc tttgcgcagc ttgagctgac
     5461 cttcgcgcga catttttttc tgacggatct tttcttcgat ctcggtcagc tcgtttttga
     5521 actcgtcgcg ctcgcccagc tctttctgaa tcgcctgcat ctgctcgttc agatacagat
     5581 ccttttgcga gcgattcatc tgctttttga cgcggctgcg gatcttcttt tcgacctgca
     5641 gaacttccag ctccgccgac aaaagctcca gcagcttggt gagccgcgcg agtggtgact
     5701 caatttccag gagcatctgc ttgtcgacaa gcttcagctg aagcgccgga atcaccgtgt
     5761 cggccagtcg ccctggctcc tcgatggttt cggtcgatcc gacgatctct tgcgggatgc
     5821 ggcggctcaa cttgatgtag ttggaaaagg cttcctgcac cttttccatc agcaccatca
     5881 cgtccgggcc aatctcttct ggttcgacaa tgtcgtcgat ttcgaccgcc aaaaacgaat
     5941 cgccaccgac gaaacgacgg acgcggaccc gctttttgcc ttcgagcagc actttgaccg
     6001 tgccgtcggg aagccgcaga agctggatca cctcaccgag cgttccgatc gagtagatgt
     6061 cggacgattc gggatcttgg gtgcgcgcac gcttttgtgc acacatcacg atcagcttgt
     6121 ctttggccac cgcttgttcg agcgcctgga tcgacttttc gcgccctacc gacagcggca
     6181 ccaccatgct tggaaacaca acgatgtcac gcagcggaag cagcggcagg acacggcttg
     6241 atcgtcgccg tccctcgccc ccatcggtct tattagagtt agccatggtg tgactttagc
     6301 atcccaattt caggccatgc aaaccaattc ccgcgcaaat tgttttgtga tttggactgc
     6361 ttagattttg gaaaatattg ggctttgcgc gcggcttgaa gatgcggttc cgcgcgaatc
     6421 tacggtaaga tggcatccac gagcagccat gagcggaaga gacaagaaca tcgtcggcga
     6481 agtcctggcc aagacctatc gcattgagcg ttttctcgga gctggtagcg tcggcaccgt
     6541 ttatgtcgct cgtcacgttc gctcgggagg tttgtacgcc gtcaaggtgc tgcaccgccg
     6601 actggcgacg agcgcggatg tgtaccagcg ctttcaagac gaagcgcggc ttatcgcgac
     6661 gctgcggcac ccgcacatct tgccgatcac cgacttcgat cgcgatgaaa gcggcgtgcc
     6721 gttttttgtg atggatctcc tcgaaggaga gagccttcag cagcgactga aacacaaaga
     6781 gacgctgccg tttgcgcaga cgatggaaat gatctcgcag gtcgggtcgg cgctgcacgc
     6841 agcccatcgc agcggcatcg tccatcgcaa cctgcgcccg gaaaacatct tcttggtgcg
     6901 gcacgatctg ggcgatcgcg tcgtcgaaac caccaaagtg accgattttg gtttggcttg
     6961 tttccgtcgc acaccgggtt ctagccgcga cgttccgcct acggtttaca acgcgcccga
     7021 gcttttgcaa gaaggcgctt ccgtcgatgg ccgtgccgat cagtgggcgc tcgcggcgct
     7081 cgcctatcgg gtgctgtcgg gcaaatcacc gtttgaagaa ggcagcgccg aagaaatcgt
     7141 cgagcgagtg accagcgaag caccgcgtcc tctcggcaag cttatgcccg agctgccacc
     7201 ccacgtcgtc gcagcgatcc atcgcggcat ggcgcgccgt cgcgaagatc gctacgaaac
     7261 gacgctcgac tttgtccgcg ccgtcaccca aaagagcagc accggcggtc acgtcgacgc
     7321 agtgccagaa aacctggttg cgcgggccag tccaccgtcg ggtcgcgtcg tcgcaccgcc
     7381 gccgccaccc gcgccgccgc cgccggaaat caaagcgccc gccgcgcccg ttccggcccc
     7441 agccccggcc cccgtcaagc cgccggtgcg acctgtgagt ggatcgaggc ctccgtcggt
     7501 gccgattccg gcattggctg caccgccacc actttttgaa aaaacgccgc cgccgatgcc
     7561 agtgccgcgc cagacctcgc agctgccggt gattttgggc ggtggtgcgg tcgtagtggt
     7621 gcttttggcg ctgatcatcg tcgtcgcggt gcgcaaaccc gcgcagcagc aaccacagca
     7681 gcagccagtc gtccagcagc cggcccaagt cgtgcagccg gtggtacagc cgatcgtcca
     7741 ggaccttgcc gtgcccgtcg tggtggccca aaatccggac gccgcgtcga accatccaac
     7801 gccagtcgtg cgtgacgtcg gactccagct gcgaggcgcc gagctgccct ccgattccgc
     7861 cgctgctgcc gtcggagctg tggcacctgc gaagccagca ccgacccctc aaggtgcggc
     7921 agtcagtccg ccaggcccga tcggaactgc atcaaatgtt ggattgaatc caaatgcggt
     7981 gaaaccggcg ggcacgcagc cagcaaatcc gacggcgacc aatccgacgg gagttgcgac
     8041 aaacaagccc tctggaactg cgacagcgac ccccgcggca ggtggtggcg atcccagccg
     8101 cgccccagcc accggaaatc cgccggcaaa tcctacgtct ccgatgacaa cacccgcggc
     8161 gggcggatcg gacagcgcga cggcggaaac caagccaagc gagccgccaa agccggctgg
     8221 tccatcgcct gaagaagtgc tcgccgacgc caaaaagacg tacctatccg gcgacaaaga
     8281 acgtgcggtg cagatggcac tgcaagccgc ggaaaaaccg gggccacatg tcattgatgc
     8341 gtggcgctgg gtcggtggcg ctgcgtgctc gattcacgac gcgagcatcg ccagccgcgc
     8401 ctacgcccat ctgggttcgc aagagcacaa aaagctgctg accgagctgt gcgagcgcag
     8461 cggcctggtg ctgcaaaacg gccagttcat caacgtcgag tagcgacagc cgccgcgcgg
     8521 gtctttgcct ggcgctttgg atgcgctacg ttgggccatg gcgctctcac tagacctcat
     8581 cgaaaaactc cccaagaccg atctgcactg tcacctcgac ggctcgctgc gccttcagac
     8641 gattctcgat ctggcgaaaa aacagggtgt gcgcttgccc gccgacacgc ccgagggact
     8701 gatgccgtat gtgttcgccg gcatgggaca tcgctcgctc gaggattacc tcaaagcgtt
     8761 cgatatcacg ctggcggtga tgcagacgga ggaagcgctg tatcgcgtcg cctacgagct
     8821 ggcggaagac tgccaccgtg aaaatgtctg gtacttggaa gttcgctatt cgccgatgct
     8881 gcacacccgc ggcggcctga gcctgacgca gatcgtcgaa tcggtgctgc aaggtctgcg
     8941 cgccgccgag cgggatttcg gcatccgcac tggcgtcatc ctctgcggca tccgttcttc
     9001 gtcggcagac tcgtcgctgc ggatggccga gctgtgcgtc gctttcaaaa accgcggcat
     9061 cgtcgcgttc gacctcgcgg gggccgaact caactacccg gccaaagcgc acaagcaggc
     9121 gttccagctc atcctcgaca acaacgtcaa ctgcaccgcc cac
//
LOCUS       DAORGN010000199         9160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3321_length_9160_cov_4.957386, whole genome shotgun sequence.
ACCESSION   DAORGN010000199 DAORGN010000000
VERSION     DAORGN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3321_length_9160_cov_4.957386"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..817)
                     /locus_tag="PKL17_17605"
     CDS             complement(23..817)
                     /locus_tag="PKL17_17605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007247371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNK46603.1"
                     /translation="MRVGIGIVVAFGMAVAAVSPAAAEVKTKEIEYKQGSTVLQGFLA
                     WDDAQKGKRPGVVIVHEWWGHNQHARNQAMRLAQAGYVGFALDMFGKGKLAKHPQDAK
                     AFVAEATKDPATARARFTAAVAQLKAAPQVDATKLAAIGYCFGGGVVLDMARQGEELA
                     AVGTFHGSLGSSFVAKKGFKPRVLVMTGAADPMIGPDAVAAFRKEMTDAGARFDVQSY
                     PGAKHGFTNPDADKAGIPGLGYSESADKASWAAWLALLAEVFPASR"
     gene            complement(839..2806)
                     /locus_tag="PKL17_17610"
     CDS             complement(839..2806)
                     /locus_tag="PKL17_17610"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46604.1"
                     /translation="MFTHVHIQNFRGLSKLSIELAQVTALIGPNSCGKTTVLQAVRLA
                     CEAVEFARKEGDLRVEKNGWISLYADRPIRDDQPFLPTARWQELFLDAKRDQPIIIEL
                     EFSADYDIERLKVELSEGRADALRMSASLKAHEGRALRALVDGAQGVRGIRAANLLSP
                     IRTQVADLVPRVMFIPAFYGVVEQEEYRAAGSVRALLQSGQQGQVVRNLLRRLPNREG
                     VNDFLNRSGLAAKLDSHQNDIDTERYLEVRFSDRNGALELSSAGTGLVSLLALFAAIE
                     TRSQLLTSKSGQAVIVLLDEPEAHLHPRLQGELASRLTTMFTSAGVQLLCATHSVEMV
                     NRFGRDERAAVLRIDRSRAGISRVLASEEERMRELDEWCDLSPFAKLNLLANRRIVFH
                     EGPSDEAIIKGCARLYLGKDPVRKKRFEDFTWAELSGCDNRGAKDVLKTALEPLFHKL
                     QPGEQVRMVRLLDCDYSRTPQFESRPSPANPQAAYQELDVIWSRHSIESLFLEPECLT
                     SWIDAALPSGSRPVSFDRAALLALVTQAIADADQDPQLCLDATKQLFPYLEKSHSKEE
                     QIRAFGRAEALVRSEPARYQRGHDRAKFVLKKIRASLEALEGTRGLANKVRADIAQIV
                     ENSPLLPNVLLPPALIPPDLAKVLDYMASEQ"
     assembly_gap    2917..3016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3259..5004
                     /locus_tag="PKL17_17615"
     CDS             3259..5004
                     /locus_tag="PKL17_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK46605.1"
                     /translation="MSIRSKLIGLLSVLLLLVLVTSTIAGRVLLKRRVDEHMRREAEA
                     TAQDLATNLEDYLKHERSDEEVKARLEALRGRHRIFDLSLIIDSESGDKVQIVLPQTG
                     SAEISHPERPRHPRSDRVLAYESRRALSDRGQPSRPPLLRGVEPQWRLPDRGASSRWA
                     QAFPPPEAPLQKQSRSINTSEGSSQCQRCVTAVADLDPMGPQRGKLRVTVPLDRYDQV
                     LRDQIGISALTALGALLVLLLSTVYIVNRVVARPVSELAQAMREVEGGNLQRRAGFVG
                     SDEMGKLSQGFNTMLDRLAKADEEIRALNARLADDILAATRDLQGKNEALQQLNQILL
                     QMQRELGDKERLAALGQLAAQLAHEIGTPLAAVSGHLQLAAFDRELPGGLRDRLQVAT
                     SELSRVSKIIRDYLDHTRAGKPSIVTADLRRLIEEAVRVTVSALRRPGIRIVTEVSPD
                     AESLQTDPGLLRQILINLLTNAIDATSAHQADAGSVATVTGRIFVSAKTEGSEVVLAV
                     RDEGTGIAPDDLQRIFEPFYTTKGRGRGTGLGLSICRELTRSLGGKITVESAPGQGST
                     FALRLPRVPQIKQAA"
     gene            complement(5009..6067)
                     /locus_tag="PKL17_17620"
     CDS             complement(5009..6067)
                     /locus_tag="PKL17_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46606.1"
                     /translation="MRAASLAVVSWLSFGGLWGSATLLSGCVDPNGNDPFDASADGST
                     DSTDGFSDQDLGASRDLTAQEGDLGKGPRVVRFAAVGDTGKGNTGQQQVADAIARKCM
                     TSGCDFVQLLGDNIYESGVASVTDPQWQTKFEVPYKDVKAPFYVTLGNHDYGGNGAGN
                     EFDKAKWEILYTASSMKWKLPANHYRRVVENADFFILDTNLIMYSREVDKQVASVKDW
                     LSSSTSTWKIAFGHHPYLSNGPHGNAGSYDGLPGVPVVDGKTVKTFLDDNVCGKVDLY
                     LSGHDHSRQWMVDTCKGTELAVSGAGASPTDLKGKNAVRWQANTLGFLYVTITDRQLV
                     AEFIDTSGKVEFTRTLNK"
     gene            6127..7362
                     /gene="serA"
                     /locus_tag="PKL17_17625"
     CDS             6127..7362
                     /gene="serA"
                     /locus_tag="PKL17_17625"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNK46607.1"
                     /translation="MSHKKTGGVPRSEPPKVLLLENIHQTAVETMAREGLVVQTLPKS
                     LPKNDLIAKLAGVQVLGIRSKTKVSEEIIRAAPDLQAIGCFCIGVNQVDLAAANRLGV
                     PVWNAPFSNTRSVAEMIIAEIIALARQLGDRSREVHERKWRKVSAGCIEVRGKTLGII
                     GYGHIGSQVSVLAESLGMRVVFYDIVPKLQLGNSQSCDSLQQLLRVSDFVTLHVPATP
                     QTQWMIGTAELSEMKKGSYLLNASRGTVVQIDALAHVLRSGHLAGAAVDVYPEEPEEN
                     SDGFVTELAGLPNVILTPHIGGSTQEAQASIGREVAQSICRFVSQGATTGAVNFPQVE
                     LPPSPGTHRIVNVHKNVPGVLRDINRVVSELNANIHSQILSTDEHIGYLIMDLDQDVS
                     GEVLGRISDLPTSIRTRIV"
     gene            7459..7878
                     /locus_tag="PKL17_17630"
     CDS             7459..7878
                     /locus_tag="PKL17_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46608.1"
                     /translation="MDSTARLSGLCAWVGAALCVLGPLSASAQQLGNAVNTAKAVVTK
                     AAPVAAKAAPVVSKASPKAGQVVQTLAGKAGAPAQSQPQAQPQPQPQAQPQPSPSLQT
                     QAVGALRNVVGGGAASPQPAAQPTAPVRRPPPGAPPG"
     gene            complement(7899..8213)
                     /locus_tag="PKL17_17635"
     CDS             complement(7899..8213)
                     /locus_tag="PKL17_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46609.1"
                     /translation="MSSAYAPQFGARFQFDRQSVSDLESHYTVRIHFASSPHDFDLRI
                     ALPGGEVTLTPVGNQAMVPAWATAFVHALGRQLFRASRKEEGWPRRIMRWHQAPTETA
                     AA"
     gene            8352..>9160
                     /locus_tag="PKL17_17640"
     CDS             8352..>9160
                     /locus_tag="PKL17_17640"
                     /inference="COORDINATES: protein motif:HMM:NF017568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HNK46610.1"
                     /translation="MKADLGKPESVSWSETASEPCEQLLASLGRSEVLTPERARRVFV
                     NRHLRMDQIEVIGFDMDYTLALYNQPRLEALSASCTLEKMIARRGYPEALRQLPYDSR
                     RAIRGLVVDRERGNVLKMDRYGYVGRAYHGTRPLPRAERRKLYREQLLRILSTHYAFI
                     DTLFALPEAVLFGQIVDFFDADPAFAALSDDKKAQRYSQLWADIRESIDEAHRDDSIK
                     RVIKADMATYIAFDPELPATLHRFRSAGKRLFLLTNSGLDYTQAVMSYLLD"
BASE COUNT         1707 a         2624 c         2923 g         1806 t
ORIGIN      
        1 ccgcaagccc cgatgcaagc ggttatcgcg acgctgggaa gacctcggca agcagtgcaa
       61 gccacgctgc ccacgatgct ttgtcggcgc tttcgctgta cccaagcccc ggaataccag
      121 ctttgtcagc gtcgggattg gtaaagccgt gcttggcacc ggggtagctc tgcacgtcaa
      181 agcgtgcgcc ggcatcggtc atttctttgc ggaatgcggc gacggcatcg ggaccgatca
      241 tcgggtcggc tgcccccgtc atcaccagca cgcgcggctt aaatcccttt ttggcgacaa
      301 agctggaccc gagcgagccg tgaaatgtcc ccactgcggc aagttcttcg ccttggcggg
      361 ccatgtccag cacgacgccg ccgccgaaac agtatccgat ggccgccagc ttggtcgcgt
      421 cgacctgcgg agccgccttg agctgcgcca cagccgccgt gaagcgtgcg cgcgcagtcg
      481 ccgggtcttt ggtggcttcg gcaacgaacg ctttggcgtc ttgcgggtgc ttggcgagct
      541 tgcctttgcc aaacatgtcg agcgcaaacc caacatagcc cgcctgtgcg agccgcatcg
      601 cttggttgcg cgcatgctgg ttgtggcccc accactcgtg gacgatgacg acgccggggc
      661 gcttgccctt ttgcgcgtcg tcccacgcca aaaagccctg aagcaccgtc gagccttgct
      721 tgtactcgat ttctttggtc ttcacctcgg cagccgccgg cgaaaccgcc gccaccgcca
      781 tcccaaatgc caccacaatc ccaattccca cgcgcatctt cgtcctccgt taccgtggtt
      841 actgttcgct ggccatgtag tcgagcactt tcgccagatc gggcgggatc agtgcaggag
      901 gcaggagcac gttgggcaaa agtgggctgt tttccacgat ttgcgcgatg tccgcccgga
      961 ccttgttggc aagaccgcgc gttccttcta gggcttcaag ggatgcacgg attttcttta
     1021 agacaaactt cgcgcggtcg tgtccgcgct gatagcgggc cggctcgctg cgaaccagtg
     1081 cctcggcgcg accgaaggcg cgaatttgtt cttctttgct gtggctcttt tccagatagg
     1141 gaaagagctg ctttgtggca tctaggcaaa gctgggggtc ttggtctgcg tcagcaatcg
     1201 cctgtgtcac caacgcgagc agcgctgcac gatcaaaaga cactgggcga ctgcccgatg
     1261 gcagcgcggc atcgatccag cttgtcagac actccggttc gaggaacagc gactcgatgc
     1321 tgtggcggct ccatatcacg tccagttctt ggtacgccgc ctgtggattt gcgggactgg
     1381 gccttgattc aaactgcggg gtccggctgt aatcgcagtc gagcagtcgt accatccgca
     1441 cctgttcacc gggctgtagc ttgtgaaaca gcggctcgag cgccgtcttg agaacatctt
     1501 ttgctcctcg attgtcgcag cctgacagtt ctgcccacgt aaagtcctcg aatcgttttt
     1561 tccgcaccgg gtctttgccc agatacagcc gcgcgcatcc tttgatgatg gcttcgtcac
     1621 tcgggccttc atggaacacg atgcggcggt tggccagcag gttgagcttg gcaaacggtg
     1681 acaaatcgca ccactcatcc agctcacgca tgcgttcctc ttcgctggcc aaaacccgcg
     1741 aaatgcccgc tcggctgcgg tcgatccgaa gcaccgcggc tcgctcatcg cggccaaagc
     1801 ggtttaccat ctcgaccgag tgcgtcgcac acagaagctg cacaccggcg gacgtaaaca
     1861 tcgtcgtaag acgcgaagcc agctctccct ggagccgtgg gtgaagatgg gcttctggct
     1921 catccagaag gacgatcacc gcctggccgc tctttgacgt gaggagctgg gaccgcgtct
     1981 cgatggcggc aaacagcgcg agcagactca caagccctgt gcccgccgaa gacagctcga
     2041 gcgcgccatt tctgtcgctg aaacggactt ccagatatcg ctcggtatcg atgtcatttt
     2101 gatggctgtc tagcttggcg gcgagccctg agcgattgag gaagtcattg acaccttccc
     2161 ggttcggcag ccgacgaagc agattgcgga ccacctgacc ctgctgtccg ctctggagca
     2221 gcgcccgcac cgaacccgcg gcgcgatact cttcttgctc gacgacaccg tagaacgccg
     2281 ggataaacat gacgcggggc acaaggtctg cgacctgggt ccgaatcggc gacagaaggt
     2341 ttgcggcgcg gatgcctcgg actccctggg cgccatctac aagcgcccgc agcgcgcgcc
     2401 cctcgtgtgc tttcagcgat gccgacatgc gcagggcatc ggcgcgtcct tcggacagtt
     2461 ctactttcag ccgctcgatg tcgtagtctg cggagaattc cagctcgatg atgatgggct
     2521 gatcgcgctt ggcatccaga aacagttcct gccagcgcgc ggttggcaaa aagggctgat
     2581 catcccgaat cggacggtcg gcatagagcg atatccagcc atttttttcg accctgagat
     2641 cgccctcctt gcgcgcaaac tcgacggctt cacacgcgag ccgcacggct tgcaggacgg
     2701 tcgtcttgcc gcagctattg ggtccaatca gggctgtgac ctgcgcaagc tcgatgctga
     2761 gctttgacaa gccccgaaag ttctggatat ggacgtgcgt aaacatgcgc agatgctact
     2821 cgtctgccgt tttgtgcgca aagagccatc catttgacct atcaccacgg tgatccacga
     2881 tccggtggcc caggaagggt cgttttgatt tgaaaannnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnntcac cacggtgatc cacgatccgg tggcccagga agggtcgttt
     3061 tgatttgaaa aaggcgagcc ggtggcatgg gaagggtcgt tttgatttga aaaaggcgag
     3121 ccggtggcat gggaagggtc gttttgattt gaaaagggcg agccggtggc actggaacgg
     3181 gtgtttcagg tgcgcagttg cgatccggtg gggtgggggc gcgatggttt gcggtaaggt
     3241 gtgggggtgc tgccttcctt gagcattcgg agcaagctca tcggcctttt gtcggtgctt
     3301 ttgctgcttg tccttgtcac aagtacgatt gccggacggg tgctgctcaa gcgtcgcgtc
     3361 gacgaacaca tgcggcgcga agcggaagcg acggcgcagg atctggcgac caacctagaa
     3421 gactatctga agcacgaacg cagcgacgaa gaggtcaaag cgcggctgga agcgctgcgc
     3481 ggtcgtcatc gcatcttcga cttgtcgctc atcatcgaca gtgaatcggg cgacaaggtg
     3541 cagatcgtgc tgccgcagac gggtagcgcc gaaatatcgc acccggagcg gcctcggcac
     3601 ccgcgatcgg atcgcgtgct ggcctatgag tcgcggcggg cgctgtcgga ccgcggtcag
     3661 ccgagccgtc cgccgcttct gcgcggggtc gaaccgcagt ggcggctgcc agatcgcggt
     3721 gcatcgtcgc gctgggcgca ggcctttcca ccgccggaag cgccactgca aaagcagagc
     3781 cgcagcatca acacaagcga aggttcgtcg cagtgtcagc gctgtgtgac ggcggtggcg
     3841 gacctagacc cgatgggccc ccagcgcggc aagctgcggg tgacggtgcc gctcgatcgc
     3901 tatgaccaag tgctgcggga tcaaatcggt atttcggcgc tgacggcgct gggagcgctt
     3961 ttggtgctgc ttttgtcgac ggtgtatatc gtcaatcgcg tcgtcgcgcg cccagtgtca
     4021 gagctggcgc aggcgatgcg ggaagtcgaa ggcggcaacc tccagcggcg ggccggattt
     4081 gtcggttcgg atgagatggg caagctttcg cagggcttta acacgatgct cgaccggctg
     4141 gcgaaggccg acgaagaaat cagagcctta aatgcgcggc tggccgacga tattttggcg
     4201 gcgacgcgcg atctgcaggg gaaaaacgaa gcacttcagc agctcaacca gattcttttg
     4261 cagatgcagc gcgagctggg cgacaaagaa cggctggcgg cgctgggaca gctggcggcg
     4321 cagcttgcgc atgagattgg gacgccactg gctgcggtgt cggggcactt gcagctggcg
     4381 gcgtttgacc gggagctgcc gggagggctg cgcgatcgtc tgcaagtggc gacaagtgag
     4441 ctttcgcggg tgtcgaagat cattcgcgac tacctggatc acacgcgggc gggcaagccg
     4501 agcattgtga cggcggatct gcggcggctg atagaagaag cggtgcgggt cacggtgagc
     4561 gcgctgcggc ggcccggcat tcgcatcgtc accgaggtaa gtcccgacgc agaatcgctg
     4621 caaaccgatc cgggtctttt gcggcaaatt cttatcaacc tgctgacaaa cgccatcgac
     4681 gcaacgtcgg ctcatcaggc cgatgcggga agcgtggcga cggtgacggg acggatcttt
     4741 gtgtcggcga agaccgaagg ttcagaagtg gtgctggcgg tgcgggacga aggcaccggc
     4801 attgctcccg atgatctaca gcgaatcttt gagccgtttt acacgaccaa aggacgtggg
     4861 cggggaaccg gtctgggcct gtcgatttgt cgagagctga cgcgatcgct cggcggcaag
     4921 atcaccgtgg agagcgcgcc gggtcaagga tcgacgtttg cgctgcggct gccgcgggtg
     4981 ccgcagatca agcaggcggc ctagcgcatc atttgttgag cgtgcgggtg aattcgacct
     5041 tgccgcttgt gtcgataaac tcggcgacga gctggcggtc ggtgatggtc acgtacagga
     5101 agccgagggt gtttgcctgc cagcggacgg cgtttttgcc cttgagatcc gtgggcgagg
     5161 cgccagcacc agaaacggcc agctcggtgc ctttgcaggt gtcgaccatc cactggcggc
     5221 tgtggtcgtg gccgctcaag tacaggtcga ctttgccgca gacgttgtcg tcgaggaacg
     5281 tcttgacggt cttgccatcg acgaccggga cgccgggaag cccgtcgtaa ctgccagcgt
     5341 tgccgtgcgg accgttggac aggtaggggt ggtggccaaa cgcgattttc cacgtcgaag
     5401 tcgagctaga gagccagtct ttgaccgacg ccacctgctt gtcgacttcg cggctgtaca
     5461 tgatcaggtt tgtatcgagg atgaagaagt cagcgttttc gacgacgcgc cggtagtggt
     5521 tggcgggcag tttccacttc atcgacgacg cggtgtagag gatttcccac ttggctttgt
     5581 cgaattcgtt gccggcaccg ttgccgccgt agtcgtggtt gccgagcgtg acgtagaacg
     5641 gggctttgac gtctttgtag gggacttcga acttggtctg ccactgcggg tcggtgacgc
     5701 ttgcgacgcc gctttcgtag atgttgtcgc ccagaagctg gacaaagtcg cagcctgacg
     5761 tcatgcactt gcgcgcgatg gcatcggcga cttgctgctg gccggtgttg ccctttccgg
     5821 tgtcgccgac ggcggcgaaa cggaccacgc gcggaccttt gcccagatcg ccttcttgcg
     5881 cggtaagatc ccggctcgca ccgaggtctt ggtcagaaaa tccatctgtc gagtccgtac
     5941 ttccgtcggc agatgcatca aaggggtcat tgccgtttgg atcgacgcag ccggaaagaa
     6001 gcgtcgcact tccccaaagg ccaccaaaag agagccaaga aaccaccgca agtgacgctg
     6061 cacgcatgcg gcagaatgtg caaaaaacca caggctttgt acagcgagca gaaagactct
     6121 caacgcatgt cacacaagaa aacgggcggc gttccgcgaa gtgagccgcc caaagtgctt
     6181 ctcctcgaaa acatccacca gactgcggtg gaaacgatgg cgcgcgaggg cttggttgtg
     6241 cagactttgc ccaagtcgct gcccaaaaac gacctgattg cgaagctggc cggcgtccag
     6301 gtgctgggca ttcgcagcaa gaccaaggtc agcgaagaga tcatccgtgc ggcgcccgac
     6361 ttgcaggcga tcggctgttt ctgcatcggc gtcaatcagg tcgacttggc ggcggcgaat
     6421 cggctgggcg tgccggtctg gaatgcgccg ttttccaaca cccgatcggt cgcggagatg
     6481 atcatcgccg agatcattgc gctggcgcgt caactcggcg atcgcagccg cgaggtgcac
     6541 gagcgaaagt ggcgcaaagt gtcggcgggc tgcatcgagg tgcgcggcaa aacgctcggc
     6601 atcatcggct atggacacat tggatcgcag gtgtcggtgc tcgccgaatc gctgggcatg
     6661 cgggtggtgt tttacgacat cgtgcccaag ctccagcttg gcaacagcca gtcgtgcgac
     6721 agcttgcagc agcttctgcg ggtgtccgat tttgtgacgc tgcacgttcc ggcgacaccg
     6781 cagacgcagt ggatgattgg cacggcggaa ctgtcggaaa tgaaaaaggg cagttatctg
     6841 ctaaatgcca gccgaggcac cgtcgtgcag atcgatgcgc ttgcgcatgt gctgcggtcg
     6901 gggcatttgg cgggtgcggc ggtcgatgtc tatcccgagg agccagaaga aaactccgac
     6961 ggttttgtga cagagcttgc cgggctgccg aatgtgatct tgacgccgca cattggtgga
     7021 tcgacgcaag aagcgcaggc cagcatcggt cgcgaagtcg cgcagagcat ctgccgtttt
     7081 gtttcgcagg gcgcgacgac gggcgcggtg aactttcccc aggtcgagct gccaccgtcg
     7141 ccgggaaccc atcgcatcgt caacgtccac aaaaacgtcc ccggcgtgct gcgtgacata
     7201 aaccgcgtgg tgtccgaact gaacgccaac atccacagcc agattctgtc gacggatgag
     7261 cacatcggct acttgatcat ggatctggac caagatgtgt ccggcgaagt gctgggccgg
     7321 ataagtgatc tgccgaccag catccgcacc cgcatcgtgt aagcccggac gctgggtgtg
     7381 aaatgtctgg gccccgacgg gacgaaagcg gtgaggcgcg cggtgggatt ttgctatagt
     7441 cgtcgagcag gaggctttat ggattccact gctcgtctgt ctggtctgtg tgcgtgggtc
     7501 ggtgctgcgc tctgtgtgct tggtccgctg tcggcttcgg cgcagcagct tggcaatgcg
     7561 gtcaacaccg cgaaagccgt ggtgacaaag gctgccccgg tggctgccaa agcggcgccg
     7621 gtggtatcga aagcgtcgcc gaaagccggg caggtggtgc agacgctggc agggaaagcg
     7681 ggcgcacccg cgcagtcgca gcctcaagcg cagccgcagc ctcaacccca ggcgcagccg
     7741 cagccgagtc cgtctttgca gacccaagcc gttggggcgc tgcgcaacgt cgtaggtgga
     7801 ggcgcggcaa gtccgcagcc tgcggcccaa ccgacggctc cggttcgtcg tccgccgccg
     7861 ggtgctccgc caggctaacc cgcccgtttt ttgcggtttt aggctgcggc ggtttccgtc
     7921 ggagcctggt gccagcgcat gatccgtctg ggccagcctt cttccttgcg cgatgcacga
     7981 aataactgcc gtccaagcgc atgcacaaac gccgtcgccc aggccggcac catcgcttga
     8041 ttgcccactg gcgtcagcgt gacttcgccg ccgggcagtg cgatgcgaag atcaaagtcg
     8101 tgcgggctcg acgcaaagtg aatccgcacc gtgtagtgag actccagatc gctgacggac
     8161 tgccgatcga actgaaaccg ggccccaaac tggggagcat acgcagatga catagcgctt
     8221 tgatagcgca gcttttgtaa acttgtccag ccattcagcc atgcgtcttg cttcctgggc
     8281 gctcctcgac gtggcatggg cttttggcga tacgaaaagt tcgcgatata gtgagcattt
     8341 ctggatatct gatgaaggcc gatcttggaa agccggagag tgtgtcttgg tctgaaacgg
     8401 cgagcgagcc gtgcgagcag ctgctggcct cgcttggtcg cagcgaagtg ttgacccccg
     8461 agcgagcgcg gcgggtgttc gtcaatcgcc atctgcggat ggatcagatc gaagtgatcg
     8521 gctttgacat ggactacacg ctggcgcttt ataaccagcc tcggctcgaa gcgctgtcgg
     8581 cgtcgtgtac gctggaaaaa atgatcgcgc gacggggcta tccggaagcg ctgcggcagc
     8641 ttccgtatga ctcacgacgc gcgattcgcg gcctggtggt ggatcgtgag cgcggcaatg
     8701 tcctcaagat ggatcgctac ggttacgtcg ggcgggcgta tcacgggaca cggcctttgc
     8761 cgcgggccga gcggcgaaaa ctgtatcgcg agcagcttct gcggattttg tccacgcact
     8821 acgcctttat tgacacgctg tttgcgctgc ccgaagcggt gctgtttggt cagatcgtcg
     8881 attttttcga tgctgaccca gcgtttgcgg cgctgtccga tgacaaaaaa gcgcagcgct
     8941 acagccagct atgggccgac attcgcgaaa gcatcgacga agcgcaccgc gacgacagca
     9001 tcaagcgggt gataaaggcc gacatggcga cgtatatcgc gtttgatccg gagcttccgg
     9061 cgacgctgca tcgctttcga tcggcgggca agcggctgtt tcttttgacc aacagcgggc
     9121 tcgattacac ccaagcggtg atgtcgtatc tgctcgacaa
//
LOCUS       DAORGN010000200         9149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3328_length_9149_cov_6.884869, whole genome shotgun sequence.
ACCESSION   DAORGN010000200 DAORGN010000000
VERSION     DAORGN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3328_length_9149_cov_6.884869"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKL17_17645"
     CDS             complement(<1..271)
                     /locus_tag="PKL17_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46611.1"
                     /translation="MTNDSRSARNSRRGCGRLGRFMLALTLASAAGVFEAKAHAQDYA
                     AAGQHFDAAQEAFAAGKFELAAKEYQAAFDITKESTLLQSIGESWQ"
     gene            360..1343
                     /locus_tag="PKL17_17650"
     CDS             360..1343
                     /locus_tag="PKL17_17650"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK46612.1"
                     /translation="MSKRKTLFATALAAAVGLSALVSIPESAIANDKKPVKDNGIPRE
                     IKTRELMAEREGLKLKLYEKWRVGNEDNWKQNGKVILLVHGATWASRCTFDPDPDHGY
                     SMMEMLADAGYDVFAVDLHGYGRSERSDIDWTDAQSAAKDVEAAVEFIRALRWVERVH
                     LFGYQWGAQPAALFASQKPHKVGRLAVLGMRYRLTDKAGNVPPGPLRTLGQQASLLKP
                     EDGDLDPEFVRRRAQLCRGESASAPNGALLDLTKPSPVDPAKIKVPTLVLQGERDGSM
                     EVVADRMQFFQQLGSPGKWFTFLPGVGKYAPIERTRSKFDQTLLSFLDQTP"
     gene            complement(1350..2687)
                     /locus_tag="PKL17_17655"
     CDS             complement(1350..2687)
                     /locus_tag="PKL17_17655"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNK46613.1"
                     /translation="MKLAFIAIALSSIDSYPSSDSVPSLAALTARVTLTALAAGGEAV
                     GRLPDGRVVFVPGSAPDELADVAIEEVRKGYVRALLRNIVRPSPHRVNPACVLATPGR
                     CGGCPLMHIAAKAQQSAKQDWVLRAVRHSGARVLPLLTPTPPLAYRVRAKLGVTGGKL
                     SFSLLRSNQRQVVSSCAVLSPQLARVLFGPADRLVSLIGDGGTLAALVGQHDGQTGVQ
                     LAVELGPAGRRSEVRDEVKRLWESGIIVGGIVGDEVIGCAALMLDDSFAPLFASADGF
                     AQASAAGHTILPALLRDAVAHGFDSPPTVLELYAGSGNLTRAICDVASQVVAVEGEPR
                     AAARLHALQKQLPRLQVHALAVEKALPLVLKSQPHFDVIVLDPPRGGARSIVSRLGGV
                     KAKRIVYVSCDAMTLGRDLVELRKLGYQPQTVQPIDLMPQTAEVECIAVLDGP"
     gene            2737..3279
                     /locus_tag="PKL17_17660"
     CDS             2737..3279
                     /locus_tag="PKL17_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNK46614.1"
                     /translation="MRRFALVCLLLVVAPLARAERREVSVGVSPIYAAGYLPDRTGNG
                     GGGVGHIQFGITDAVSILAFGGASYHPLPADPDKKLLEGSLLAWHASVGVAYALDIVR
                     IQPFFEAAVGVISLTSKIGKDVSGGLQAGVAVGVGADYLLNRRFSFGFAFRYHGVLTD
                     LGNIPLYLTVGPRAQVRFWM"
     gene            3283..4215
                     /locus_tag="PKL17_17665"
     CDS             3283..4215
                     /locus_tag="PKL17_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK46615.1"
                     /translation="MPGQLVDVQRRKLRRLMTLVAIFAYGPSALLITIGVLILVFGHA
                     TKDVVFGVMTLSLTVTLLGGVALTIVYVRRESSVAQLQTEFVNKVSHDLRTPLTSIRL
                     FVETLQAKRLQDPEKIQQVLDIIATETKRLSEMVERLLGWARMEAGRREYHYVDQGPS
                     EVIDVALRAFEPQVLTYDPGKIRIEKQLGDDLPKVRVDVPAMSEALLNLLQNAFHYTG
                     PDKIITVRAQKRGQQVAISVSDNGPGIPKHEQRRVFEKFYRLTRPEMPLQPPGTGLGL
                     SMVQHIVAAHDGSVTVDSEPGHGATFTVLLPGQL"
     gene            4240..4938
                     /locus_tag="PKL17_17670"
     CDS             4240..4938
                     /locus_tag="PKL17_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK46616.1"
                     /translation="MSETILLVEDDVSIRKALQLNLQLEGYDLLCAEDGETGRRLSQT
                     ENLDLIVVDLMLPRLSGLDLIRDLRQRRADLPILVLSAKDQEADKVMALSLGADDYMT
                     KPFGLAELLARVRALLRRGRLNKPLSSSPSLSGGKASSRIYLDVPGRRLLVDGDDIET
                     TAKEFDLLKLFMSHPGQVLTREQIMQRIWGGEHFTLRTIDNFVVRLRSKIEENPDTPA
                     HLETVRGVGYRFNP"
     gene            5042..6616
                     /locus_tag="PKL17_17675"
     CDS             5042..6616
                     /locus_tag="PKL17_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46617.1"
                     /translation="MPADNWLFTKRFRSCWLLLVLLGPLLLGTSVGRAQQPSVVAMAP
                     FDGRELGKPPKKPIEPSFTASVVFEALQARSQNKPYRVLAPDLWTGIARVKHHGRVTD
                     VVGVAQLLSARLVVAGYLEATSGSEYAKPYRLNVSVYGADGALLGQLSFDLDRPLLQP
                     AAFDDKAAAIQQLIEQSLQRQPLIAAPAVPQPVTMGKNRGKPMPVSGPSQAVAVAQAD
                     DSEPVRFDPSQPKPMLVPLSEEAREQLLRRPPWQAAIDLQVGYVYSTRLLANEGTELR
                     FGRSGAHGMVLLGEVHPLALLPTVGPWLSGLGLRAQVVLPFWPDIAHVMQVGQPQTGT
                     YTASEYRYDIALREHINPWNALLRPDIAVELLYGEHSFTTAAKTNIDYLRVPPSDYRY
                     LGGALHLRMFFTQRISLTVGAMAAKLLSLGLMSRSGNDMGNMIAARDRNGFLSYGSGD
                     GFQWRLEAASQVNVYRGLTLGVRFFYEQNRLSFDGDGNVLQANGQPVTAATDDYLGVM
                     AHIGYVFQPRLRTPNQ"
     gene            6744..7169
                     /locus_tag="PKL17_17680"
     CDS             6744..7169
                     /locus_tag="PKL17_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46618.1"
                     /translation="MTSPSQRALVVDDSPTIRNFVTATLEAAGYEVTTAKSGFEALKI
                     LPGASFHLVVTDVNMPDINGLELVRYVRNSPVHKGTPLIIISTDGRERDRERGMHLGA
                     SAYLVKPFQPEALVKLAKEVTAPGFHVAPQMVAQPSKKA"
     gene            7225..>9149
                     /locus_tag="PKL17_17685"
     CDS             7225..>9149
                     /locus_tag="PKL17_17685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNK46619.1"
                     /translation="MAEQGSDKLRDEFLSESQEIIEQLNRDLLAIADACLGRDPSTEG
                     IRVDPDLINSAFRAIHSLKGLAGLSGVERMMHMAHHVENLLDALRLGKVLLTPHVLDL
                     LFESIEHAQLICAEVADHGLLAASPPSALVDDFTGRVNRAANPEGKGAQKGGEPSYGD
                     FDLDPGLVAALTEYEEHRLKENIRTGKNLYRILGSFPLMSIDVELENIKASLKPVGEV
                     IAIVPGSSGTDPERIDLEVILGSQHGSSAIVGAVGNAPARVMQVTRRSATAASSVSIH
                     PDMPSGGSPPSANRQSVTVRQSVNVPVPPPVEKGQPPLPPKEPPPGSDGDGDGGDGGG
                     NRQEPGHTLKSVSQTVRVDIRKLDVLMNIVGELALTRSGMQVVHDELRRDRVRAELAR
                     ALQDELRALTRKLDELQAGILAVRMVPLQQVFDKLSRVVRRMSREAIGKDIHFVVAGG
                     DTKLDKLIIEELSDPLMHLIRNAIDHGIEDQQLRLARGKPAAGTVAINAEQRGNHVVL
                     EIEDDGNGIDTQALVQRAIARGQISEDQARDMTRHEMYELMFLPGLSTRAVADETSGR
                     GVGMDVVKTNISRMAGIIEVDSEPGHGTRLTITLPITLAIIQALVIRAAGRTYAIPLN
                     SVMESLMLDYKQIRTIER"
BASE COUNT         1807 a         2693 c         2854 g         1795 t
ORIGIN      
        1 gctgccacga ctcgccgatg ctttgaagga gggtcgactc tttggtgatg tcaaacgccg
       61 cttggtactc cttcgcggcc agctcgaact ttcccgccgc gaacgcttct tgcgccgcgt
      121 cgaagtgctg tcccgccgcc gcgtagtctt gcgcgtgcgc ttttgcttcg aaaacgccag
      181 cagcagaagc gagcgtgaga gcgagcataa agcggcccag tcgaccacag cctcgtcgcg
      241 aattccgtgc gcttctcgaa tcgtttgtca tcggggccgc catcctaaca aaagcgtctt
      301 ccttacaccc actcgttttg ttgctgaaga gggtgtttcg gtgatatgcg aaaggcccca
      361 tgtcgaagcg taagactctc tttgccaccg ccctggccgc cgcggtggga ctttccgcat
      421 tggtttcgat tccagagtcg gccattgcga acgacaaaaa gccggtcaaa gacaacggca
      481 tcccgcgtga aataaagacg cgcgagttga tggcagagcg cgaagggctc aagctcaagc
      541 tctacgaaaa gtggcgcgtc ggtaacgaag acaactggaa gcagaacggc aaggtgatcc
      601 tgctggtcca tggtgccacc tgggccagcc gctgcacgtt cgatccagac ccggaccacg
      661 gctactcgat gatggaaatg ctagccgatg cgggttacga cgtgtttgcc gtcgaccttc
      721 atggctatgg acgttccgag cgcagcgata tcgactggac cgacgcgcag agtgcagcaa
      781 aagatgtcga agccgcggtc gagttcattc gtgccctgcg ctgggtcgag cgggttcatt
      841 tgtttggcta ccagtggggg gctcagcctg ccgccctgtt tgccagtcaa aagccgcaca
      901 aggtgggtcg tctggcggtg cttggcatgc gctacaggtt gacggacaag gctggcaacg
      961 tgccgccggg gcctctgcgc acgctcggtc agcaagcgtc gcttttgaaa ccggaagacg
     1021 gcgacttgga cccggaattt gtgcgtaggc gggctcagct ctgccgcggc gagagtgctt
     1081 cggcgccaaa cggtgcgctg ctggacttga cgaagcccag cccggttgac ccagcgaaaa
     1141 tcaaagttcc gacgctggtt ttgcaaggcg agcgcgatgg ttcgatggaa gtcgtagccg
     1201 accgcatgca gttctttcag cagctcggaa gccctgggaa gtggttcaca ttcctgccag
     1261 gggttggcaa atacgctcca atcgagcgca cgcgcagcaa gttcgaccag acgctgctta
     1321 gcttcctcga ccaaacgccg taagcgctgc tacggaccgt caagcaccgc gatgcactcg
     1381 acttcggcgg tctgtggcat caggtcgatg ggctgcacgg tctgcggctg atagcccagc
     1441 ttgcgaagct cgacaagatc ccgtccgagc gtcatcgcat cacacgacac atagacgatt
     1501 cgcttggctt tcactccgcc cagccgcgaa acgatcgagc gcgctccgcc acgcggcggg
     1561 tcgagcacga tgacatcgaa atgcggctga cttttgagca cgagcggcag agctttttcg
     1621 acagccagcg cgtgaacctg tagccgcgga agctgttttt gaagcgcatg cagacgggcc
     1681 gccgcgcgcg gttcgccttc gacagccacg acctgggatg ccacatcgca gatcgcgcgg
     1741 gttaggttgc cacttcccgc atacagctca agaaccgtcg gaggactgtc aaaaccgtgc
     1801 gccaccgcat cccgcaaaag tgcgggaagg attgtgtgtc cggcggcgct ggcctgggca
     1861 aaaccatcgg cgctggcaaa aagcggagca aatgaatcgt cgagcatgag cgcagcacag
     1921 ccgatcactt catcaccaac gatcccgccg acgatgatcc cggattccca gagtcgcttg
     1981 acttcgtcgc gcacctcgct tcgacggcct gctgggccca gctcgacggc aagctgcacg
     2041 cccgtttgtc cgtcatgctg acccacaagt gcggccagcg ttcctccgtc tccgatgagc
     2101 gataccaatc gatcggcagg accgaacaga acacgcgcaa gctgcggact taggaccgcg
     2161 caagacgaga cgacctgccg ctgattcgat cgcaagagcg aaaagctaag cttgccgccg
     2221 gtcacgccca gcttggcgcg gacacgataa gcaagaggcg gcgtgggcgt tagcagtggt
     2281 agcacgcgcg caccggaatg acggacagcc cgcaaaaccc agtcctgttt ggcgctttgc
     2341 tgggcttttg ctgcgatgtg cataagtggg cagccgccac agcgacccgg cgttgccagc
     2401 acgcacgccg gattcacccg atggggagac ggacgcacaa tgttgcgcag cagagccctc
     2461 acgtagcctt tgcgcacttc ttcgatggcg acatcggcga gctcatcggg ggcagaaccc
     2521 ggaacgaaga ccacccggcc atcgggaagc cgtccgaccg cttcccctcc cgcggcgagc
     2581 gcggtcagcg tgacacgcgc ggtaagcgct gccaaagacg gcacagaatc tgacgacgga
     2641 tacgaatcga tgctggacaa agcgatggca ataaaggcca gcttcacaaa aagcgcaagt
     2701 tgctcctcgg gtgcgctcgt gttacgagcg acaagaatgc gacgcttcgc actggtctgt
     2761 ctgcttctgg tcgtagcccc gctggcgcgt gccgagcgcc gcgaggtcag cgtgggcgtt
     2821 tcgccaatct atgcggctgg ctacttgccc gacaggactg gcaacggcgg cggcggtgtt
     2881 ggccacatcc agttcggcat caccgatgcg gtgtcgatac tggcgttcgg cggcgcttcg
     2941 tatcacccac tgcctgccga tccggacaaa aaactgctcg aaggaagctt gctggcctgg
     3001 cacgccagtg tcggagttgc ctacgcgctc gacatcgtgc ggatacagcc attctttgaa
     3061 gcggcagttg gcgtgatctc tctgacgagc aagattggca aagacgtaag cggcggtctg
     3121 caagctggcg tggcagtcgg cgtcggcgcg gactacctgc taaaccggcg gttttcgttc
     3181 ggttttgcgt ttcgctatca cggcgtgctg accgatcttg gcaacattcc tctttacctt
     3241 accgtcggac cgcgcgcgca ggtccgattt tggatgtaac ctttgccggg tcaacttgta
     3301 gacgtacagc ggcgcaagct tcggcggctg atgacgctgg tcgccatctt tgcgtatgga
     3361 cccagtgcgc tgcttatcac catcggcgta ctgattttgg tgtttggcca cgcgaccaaa
     3421 gacgtggtct ttggcgtgat gacgctgagc ttgacggtga cgcttctcgg cggagtggcg
     3481 ctgacgattg tctacgtgcg gcgtgagtcg agcgtggcgc agcttcagac cgagttcgtc
     3541 aacaaggtgt cacacgacct gcgaacgccg ctgacctcga tccgactgtt tgtcgaaacg
     3601 ctgcaggcca agcggttgca agacccggaa aagatccagc aggtgctcga catcatcgcc
     3661 acggaaacca agcggctgtc cgagatggtc gagcgcctgc ttggctgggc tcgcatggaa
     3721 gctggccgtc gagagtatca ttatgtcgat cagggtccgt cggaagtgat cgatgtggcg
     3781 ctgcgcgcct ttgagccgca ggttttgacc tacgatccgg ggaaaattcg catcgaaaag
     3841 cagcttggtg acgacttgcc caaagttcgt gtcgatgtgc cagcgatgag cgaagcgctg
     3901 ctcaatctgc ttcagaacgc atttcactac accggtcccg acaagatcat cacggtgcgg
     3961 gcgcagaagc gagggcagca ggttgccatc agcgtatcgg acaacggtcc agggattccc
     4021 aaacacgagc agcgtcgagt attcgagaag ttctaccgct tgacgcgccc cgagatgccg
     4081 ctgcaaccgc cgggcacagg tcttggcttg tcgatggttc agcacattgt cgcggcgcat
     4141 gatggatccg tcacggtcga cagcgagcct ggtcatggcg cgacgttcac tgtgcttttg
     4201 ccgggtcagc tttaggctac cttttcgtct gagaaaccga tgagcgagac gatccttttg
     4261 gtcgaagacg atgtgtcgat ccgcaaagcg ctccagctca acttgcagct cgaaggctat
     4321 gacttgctgt gcgcagagga tggcgagaca ggccgtcggc tgtcgcagac cgaaaacctc
     4381 gacttgattg tcgtggactt gatgctgccg cggctttcgg gtctcgactt gattcgtgac
     4441 ctgcgacaaa ggcgagccga cctgccgatc ttggtgcttt ccgccaaaga ccaggaagcc
     4501 gacaaagtga tggcgctgtc gctgggcgcc gatgactaca tgaccaagcc gtttgggctg
     4561 gctgagcttt tggcgcgggt gcgcgcgctt ttgcgacgcg gtcgattgaa caaaccgctg
     4621 tcgagtagcc cgagcttgag cggtggcaaa gcgtcgtcgc gaatttactt ggatgtaccg
     4681 ggacgacggc tcttggtcga tggcgatgac atcgagacca cggcaaaaga gttcgatctg
     4741 ctgaagctgt ttatgtcgca tcccggccag gttttgacgc gggagcagat catgcagcgc
     4801 atctgggggg gcgagcactt tacgctgcgg acgattgaca actttgtggt gcggctgcgc
     4861 agcaagattg aagaaaaccc cgacacgccc gcgcatctgg aaaccgtgcg tggcgtcggc
     4921 taccgcttca atccctagcc cacatgcaca gcccacgatc acaaagctcg gaaagctaca
     4981 aattccccta gaagctgtgg caacatagtt tgcactcgga agtgtgtccg agcgatttgc
     5041 tatgcccgca gacaactggt tattcaccaa gcggtttcgc tcttgctggc ttctgctcgt
     5101 gctgctgggt ccgctgcttt tgggaaccag cgttggtcgc gcgcagcagc cgtctgttgt
     5161 tgcgatggct ccgtttgatg gacgtgagct tggcaagccg cccaaaaaac cgattgagcc
     5221 gagcttcacc gcgagcgtgg tgttcgaggc actgcaagcg cgcagccaaa acaagccgta
     5281 tcgggtcttg gccccagacc tgtggaccgg catcgcgcgc gtgaaacatc acggacgggt
     5341 gactgacgtg gtgggcgtgg cccagctttt gtcggcgcgg cttgtggtcg cggggtatct
     5401 ggaagcgacc agtggttcgg aatacgccaa gccgtatcga ctgaacgtat cggtgtatgg
     5461 cgcagatggt gcgcttttgg gacagctttc atttgacctg gacagaccgc tgctgcagcc
     5521 cgcggctttt gacgacaaag cggcggcgat tcagcagctc atcgagcagt ccctacagcg
     5581 tcagccgctc attgctgcgc cggctgtgcc gcagccagtg acgatgggaa aaaaccgcgg
     5641 aaagcccatg cctgtcagtg ggccgtcgca ggccgttgcg gtggcgcagg ccgatgacag
     5701 tgagccggtg cggtttgatc cgagccagcc caagccgatg ctcgtaccgc tttcagaaga
     5761 ggcgcgcgag cagctacttc gccgtccgcc ctggcaagcc gccatcgact tgcaggtggg
     5821 ttatgtctac agcacgcgac tgcttgccaa cgaaggcacg gagcttcgct tcggtcgctc
     5881 cggggcgcac ggcatggttc tgctcgggga agtccatccg ctggcgctgt tgcccaccgt
     5941 ggggccgtgg ctcagcgggc ttgggctgcg ggcgcaggtg gtgttgccgt tttggcccga
     6001 catcgcgcat gtgatgcagg ttgggcagcc gcagacggga acgtacaccg ccagcgagta
     6061 ccggtacgac attgccttgc gagagcacat caatccgtgg aacgcgcttt tgcggcccga
     6121 cattgctgtc gagcttctgt acggcgagca cagctttacc accgcggcga agacgaacat
     6181 cgactatctg cgggtgccac cgtcggacta tcgctacctg ggcggcgcgc tgcacctgcg
     6241 aatgttcttc acccagcgaa tttcgctcac cgtgggggcg atggctgcca agctgctcag
     6301 cttgggcctg atgagccgct cgggcaacga catggggaac atgatcgcag cccgcgaccg
     6361 caacggcttt ttgtcttatg gaagcggcga tggcttccag tggcggctag aggccgcgtc
     6421 acaggtcaat gtctatcgcg ggctgacgct gggcgtgcgc tttttctatg aacagaaccg
     6481 cctcagcttt gatggagacg gcaacgtgct tcaggcaaac gggcagccgg tgactgccgc
     6541 caccgacgac tatctgggcg tgatggcgca catcggatac gtgttccagc cgaggcttcg
     6601 cacgccgaat cagtaagccg cgctggctgc ctccgtctcg tttgtctcgc cgctgttcct
     6661 cgccatgcga cttccgatca aaactccatc tatttgttag ttagaaacca aaaaatgttt
     6721 gaccagtgat atagttacgc tccatgacgt ccccttctca gcgcgcgctg gtcgtcgacg
     6781 attcaccaac catccgcaac ttcgtaacag cgactctgga agccgccggg tatgaagtca
     6841 ccaccgccaa gagtggcttc gaagcgctca agattctgcc gggcgcttcg ttccacctgg
     6901 tcgtcaccga tgtgaacatg ccggacatta acggcttgga gctggtgcgc tacgtccgca
     6961 acagcccggt gcacaaaggc acgccactta tcatcatcag taccgacgga cgggagcgtg
     7021 atcgcgagcg tggcatgcac cttggcgcgt cggcgtatct ggtcaagccg tttcagccgg
     7081 aagcgctggt caagctggcc aaagaggtga cggcgccggg ctttcacgtc gcgccgcaga
     7141 tggttgccca gccttctaag aaggcctagc tcggcggacc accgcgtctt gtcggtggtt
     7201 tgcaaaggtg ggtaaaagcg gatcatggcc gagcagggca gcgacaagct acgggacgag
     7261 ttcctgtcgg aatcgcagga gatcatcgag cagctcaacc gagatttgct cgcgattgcc
     7321 gatgcgtgct tgggtcgcga tccgtccacc gaaggaatcc gcgtcgatcc ggaccttatc
     7381 aacagcgcgt ttcgggcgat ccactcgctc aagggactgg ctgggctgtc gggtgtcgag
     7441 cggatgatgc acatggcgca tcacgtcgag aatctgctcg atgcgctgcg gcttggcaaa
     7501 gtgcttctga ccccgcatgt gctcgatctg ctgttcgagt cgatagagca cgcccagttg
     7561 atttgcgctg aggtcgccga ccacggactt ctggcagctt cgccaccgag tgcgctggtc
     7621 gatgacttca cgggccgtgt caaccgcgcc gccaatccag aaggcaaggg cgcgcaaaaa
     7681 ggcggcgagc cgagctacgg ggactttgac cttgatcctg ggctcgtcgc agcactaacg
     7741 gaatacgagg agcatcggct caaagagaac attcggacgg gcaagaacct gtaccgcatc
     7801 ttgggctcgt ttccgctcat gtcgattgac gtcgagctgg aaaacatcaa agcctcgctc
     7861 aagccggtcg gtgaagtgat tgcgattgtt cccggttcat cgggtacaga tccggaacga
     7921 atcgacctgg aagtcatctt gggctcgcag catggctcat cggcaattgt cggtgcagtc
     7981 ggcaatgcgc ccgcgcgtgt catgcaagtg acccggcgct cggcgacagc agccagttcg
     8041 gtgtcgatcc atccagacat gccgtcaggt ggcagtccac cttcggcaaa tcgtcagtcg
     8101 gtgacggtgc gtcagtcggt aaatgtcccg gtgccgccgc ccgtcgaaaa aggacagccg
     8161 cctctgccac ccaaagagcc accaccgggg agtgatggcg atggcgacgg cggtgatggt
     8221 ggtggcaacc ggcaagaacc cggtcacacg ctcaagagtg tttcgcagac ggtgcgagtt
     8281 gacattcgca agctcgacgt actgatgaac atcgtcggtg agcttgcgct gacgcgatcg
     8341 gggatgcaag tcgtccacga tgaactgcgt cgtgaccgcg tccgggccga gcttgcgcgg
     8401 gcgctgcaag acgagctgcg cgcactcacc cgcaagctcg atgagctaca ggccggcatc
     8461 ttggcggtgc gcatggttcc gcttcagcag gtgttcgata agctgtcgcg cgtcgtgcgg
     8521 cggatgtcgc gggaagcgat tggcaaagac attcactttg tcgtggccgg cggggacacc
     8581 aagctcgaca agctaatcat cgaggagttg tcggacccgc tgatgcacct gattcgcaat
     8641 gccatcgatc acggcatcga ggaccagcag cttcgtcttg cgcgtggcaa acccgctgcg
     8701 gggaccgtcg ccatcaacgc cgagcagcgt ggcaatcacg tcgtgctcga aattgaagac
     8761 gatggcaatg gcatcgacac gcaagcgctg gtgcagcgag cgattgcacg cggacagatt
     8821 tcagaagacc aagcccgcga catgacccgg cacgagatgt acgagctgat gtttctgccc
     8881 ggtctttcga cgcgcgccgt cgccgacgaa acgtctggtc gtggcgttgg catggatgtc
     8941 gtcaagacca acatctcgcg aatggccggc atcatcgaag tcgattccga gccagggcat
     9001 ggcactcggc ttacgatcac gctgcccatc acgctggcca tcatccaggc gctggtgatt
     9061 cgggccgcgg gtcgcacgta tgcgattccg ctcaactcgg tgatggaaag cttgatgctc
     9121 gactacaagc agatccgcac catcgagcg
//
LOCUS       DAORGN010000201         9132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3339_length_9132_cov_5.811612, whole genome shotgun sequence.
ACCESSION   DAORGN010000201 DAORGN010000000
VERSION     DAORGN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_9132_cov_5.811612"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1433)
                     /locus_tag="PKL17_17690"
     CDS             complement(240..1433)
                     /locus_tag="PKL17_17690"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNK46620.1"
                     /translation="MSQLWAGFGTSTVADTQAAIHAAVRAAKAQLGDRSPGLAVVTAT
                     VEHDGRAIFAALRESLPNVPIHGVTTSLGVLTKQGIVTGADGAVAVLLFASSGNISFS
                     VASSVLGDDARAAGRRAAEQIARSAQPPRVLLFNGSPGREEDTLAGIAEVLPGIPAFG
                     GSAADHAIVGEWWVFANDGAHRDAVSIAALCGDVAFGGSLLAPFTETSAQAKVTAGQD
                     RHIVSLDGRPAAEVLNQWLDDGLALQLSEGGNILAQTALRPLAIRHETAIGEHFVTIH
                     PSQIHAEDRSIDLFARIQPGENVCMMTGTVEGLTRVLSELHVRAGEKLATEKTRAAIL
                     IYCAGCAGAVGGALHDALSTHLGSALADVPLLGMCTFGEQGFIPGLGNVHQDLSVSLL
                     LIGEN"
     gene            complement(1491..1706)
                     /locus_tag="PKL17_17695"
     CDS             complement(1491..1706)
                     /locus_tag="PKL17_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46621.1"
                     /translation="MESQIYRIVVFVESRLTPFAVCRLIMLSGVSVRKYGRVSIDDPQ
                     DLRKVRAALRALLNPRELGEIDALLAR"
     gene            complement(1709..2965)
                     /locus_tag="PKL17_17700"
     CDS             complement(1709..2965)
                     /locus_tag="PKL17_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNK46622.1"
                     /translation="MQFQFRTAGQLIFGRGEHRRAAQLAVGLGKRVLLVTGASSLSQN
                     GVLARIAADLGDAAEHVEHVTVSGEPDVAATDALVRQCRESGCQVVLAIGGGSVLDVG
                     KAVAALSQNAGTAIDYLEDIGRGAPLPIVQTPLPVIAMPTTAGSGSEVTRNSVLKVPD
                     LKVKRSLRGDQLLPRIAVVDPELSATAPQRVAAAAGMDALTHLIEGYLSKGAQPITDA
                     LVLRGVGKLWHALHRLAQSYAATDAGGAALPSEVHDDLALASMWGGIVLANAGLGAVH
                     GLVAPLGGLCDVAHGDGCACLLPATFLANAKALSRRQPDGVALVRMRELCALLTDGQT
                     DLELLAERLSLLRSRLGIRSLSEQGVQSAQFAQIVSQSRKGSMRSNPIELSDEELHNI
                     LRTSWQETSTMNASHHRESSSEQQVK"
     gene            complement(2979..3281)
                     /locus_tag="PKL17_17705"
     CDS             complement(2979..3281)
                     /locus_tag="PKL17_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNK46623.1"
                     /translation="MYVVVVRISVIADRVSDFVSATLANAHATRQEPGNVRFDVLQQN
                     EDPTRFTLYEVYHDEAGFKSHQQTPHYLLWRQTVADMMASPRVGEKHHSLFPSPFH"
     gene            complement(3302..4114)
                     /locus_tag="PKL17_17710"
     CDS             complement(3302..4114)
                     /locus_tag="PKL17_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46624.1"
                     /translation="MRLSRVVFSSWLLLLAVFSSGCTMFNRLVQDAVGSGIQQQRANP
                     PVVTAGSPQLVRAPTLQALAAYYCPQVVTDRMLRMGCSIGLGSPPPQQQLQFEFAVPV
                     TIKNPNNIPVPALDVLLALKLFPGQNAEALGSVCVSMCGGNDPSCTGQPKPGACTATN
                     GGIRTASDFVRAIPGLIVGLANGSLVNELRKSLIPAGGDVHLNLAFPLGIDQALRVIQ
                     NVIQPIVENLLRQQRNSLEIPVAAEGTVFVNLPVIGRLGVPFGPIQSVWRVM"
     gene            4362..5189
                     /locus_tag="PKL17_17715"
     CDS             4362..5189
                     /locus_tag="PKL17_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46625.1"
                     /translation="MPLSLCLGEAYAAALPELSAGQQQLLLSTGEEKPYNVRLHFLKS
                     NERRHDLFFSVLSRLGGGYLGVGADQNYTLAAVANAELVFLVDIDGEVIRWHKIYAAL
                     IPVAPSPQDLLKLLQARADAQAKEALEQRWPTEAAELLQSYRNYRHMLGIHLQNEMQV
                     RHNGRPSTWISDPVLYARIRALMIARRVIARVGDLHGERTMLSIGDAAHALHVTIRTV
                     YLSNVEQWFGRVYRLACVPHVLPFSVGNFRSCLNRYTSVGLEVVDHSWTTCKRGCDG"
     gene            5182..6291
                     /locus_tag="PKL17_17720"
     CDS             5182..6291
                     /locus_tag="PKL17_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNK46626.1"
                     /translation="MGDIIKKMKGEKFLGWYVRYVDADGCRKQRASHQPTKPLARKFL
                     IEIEARVARGQIGIPEPSPPPRPELTLAELCERYVQDYNRPRIKNLALYRTEQKSALK
                     RILPILGTKPVSAVTAHDIRRVRDQLSTRYKPNTVMSSLRPLSTAFSWGKREGLIDCP
                     NPCLGVERPRRESLLEFLDKDELSRLVSAAEAKARASDTFRDWLRYVAIAFTVRTGLR
                     KGELFGLRWRDLDLTTRRLDVARSYQHTPKSGTTRHLRLPSQLVPILAEWKRMCPATP
                     DDLVFPVMGRWSLRMGRKIDMLGLPELLADIGVRPLRREWHALRHTFASHFVMSGGNI
                     LTLQKILGHADTKMTLVYAHLAPDFLGDEMDRITI"
     gene            complement(6288..6710)
                     /locus_tag="PKL17_17725"
     CDS             complement(6288..6710)
                     /locus_tag="PKL17_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46627.1"
                     /translation="MARLRDISDDTRVIDLTVADLLDLLTVRLGLGQARREQGTSEPE
                     GLLDTWQTAKLLGIFPRQELPPEPPAGTAEYRAWRRKEQSLRNQVARRFQTWLDRHPE
                     VAQLAVKPKGERRRYFRRIDIEGYLGRVAVPMSRRRTG"
     gene            complement(7038..8525)
                     /locus_tag="PKL17_17730"
     CDS             complement(7038..8525)
                     /locus_tag="PKL17_17730"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK46628.1"
                     /translation="MHEQPATQSSELDRLDRDAEHVMPQELPTQDSALEDSVGQLSRV
                     QSKLVDTLHALQLLPPHLVKDHNRAVRAIQEALTLVGDTDDSEAIGPIDWVKFGERLQ
                     KRRTAAGLTQEWLADRVGVTGTTIRQLEHHTRRARRSLMLKLLAVPELNLRVSDIELS
                     AEMSAGYRHTPTSWLGSTYDPVSMVTELAETLNGPTGQLEQTLVYIDHHSAKDWLATC
                     SSPDYGSEFRETLPLGTVARRVADLIGEQALTVDALGCGDGKTESMLVKHLQMQLRMP
                     KQTELFLLDVSHSLLNTAYRHCRETLPDIRTYTIHGSFLDLPKLPMLVNPLRGRTRLF
                     AMLGYTLVNLSDELRFFRDTLSCCEPGELFLFDIQIARAPASERQKILKLDPIGNGKL
                     RPTHVNWLSGPIRRYCLGAEDIELRWELAPPGGVPGSYGFDAIAQVKMRDGRPDRRFL
                     MWRVRRYDPKLLSDSLAELGWKTIERIDYGPDGKKTLALMLLQKQ"
     gene            8760..8894
                     /locus_tag="PKL17_17735"
     CDS             8760..8894
                     /locus_tag="PKL17_17735"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNK46629.1"
                     /translation="MEKRSYRRFTAEEKRTILEETRQPGVTMAEVCRKHSVSPALIYQ
                     "
     gene            8911..>9132
                     /locus_tag="PKL17_17740"
     CDS             8911..>9132
                     /locus_tag="PKL17_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46630.1"
                     /translation="MAQQATTAALQQVDSRGKSQPDAQTARLLAELERMRRVVAEITA
                     ENLELKKNSKAGRKSPFERAAETGSAGGGR"
BASE COUNT         1828 a         2883 c         2731 g         1690 t
ORIGIN      
        1 gagatgatgt accgcaatgc gcagggagcc ccaggcgcgg cgggtgcaga tccaaccgca
       61 caaggcgccc catcgtcggg tggagcccgc ggcggcaaaa aggacgacgg cgtgatcgac
      121 gccgagttca ccgaagagaa gtagtccgac ccacgcccag aagtcactgg gcgcactgca
      181 caaatccccc gctcattaca aaccgccgtc ggcccagcgt acatgccaga ccgtggcggc
      241 tagttttcgc cgatcagaag caggctgacc gacaaatctt ggtgcacgtt gccgaggccg
      301 ggaataaatc cctgttcacc gaacgtgcac atcccaagga gcggtacatc tgcaagcgcc
      361 gaccccaagt gggtgctcag cgcatcgtgg agcgccccac cgacggcacc tgcacagccc
      421 gcacagtaaa tgagtatcgc cgctcgggtc ttttccgtcg cgagcttttc ccccgcccgc
      481 acatgcagtt ccgacagcac gcgcgtaagc ccttcgacgg tgccggtcat catgcagaca
      541 ttttctcccg gctggatccg cgcaaacagg tcgatgctgc gatcctcggc gtgaatctgc
      601 gacggatgga tcgtcacaaa gtgctcgcct atcgccgtct catgacgaat cgccaaaggt
      661 cgcagcgccg tctgcgccaa gatgttgcca ccttccgaaa gctgcagcgc caatccgtcg
      721 tcgagccact gattcagcac ttccgccgcc ggcctgccat cgagagagac aatgtggcga
      781 tcttggcccg cggtcacttt tgcttgcgcg ctcgtctcgg taaacggagc cagcagcgaa
      841 ccaccaaacg caacgtcgcc gcacagagcc gcaatagaca ccgcgtcgcg atgcgccccg
      901 tcattggcaa aaacccacca ctcaccgacg atcgcgtgat cggcagcgct gccaccaaac
      961 gcgggaatgc caggtagcac ttcagcgatc ccagcgagcg tgtcttcttc tctccccggc
     1021 gagccattga agagcaaaac ccgtggcggc tgggcgctgc gcgcgatctg ttccgccgcc
     1081 cgacgacctg ccgcccgcgc atcgtcacca agcaccgagg acgcgacgga aaaactgatg
     1141 ttgccgctgc tggcaaacag cagcacagcg acagcaccgt cggcccctgt cacaatgcct
     1201 tgcttggtca gcacgcccag cgacgtggtg acgccatgaa tcggaacatt cgggagcgac
     1261 tctcgaagtg ctgcaaaaat tgccctgcca tcgtgctcga ccgttgctgt aaccaccgcc
     1321 aagcccggac ttcggtcgcc gagctgcgcc ttggcggcgc gaactgctgc atggatcgca
     1381 gcctgggtat cagcgacggt actcgttcca aatccagccc aaagctgact catgttcgtc
     1441 tccttgcgtg agaacgtcct gcgcaaaaca aaaccaaaca gcctgctcac tcagcgcgcg
     1501 agcagtgcat caatttcacc gagttcgcgc gggttcaaaa gcgcacgcag cgccgcccgc
     1561 actttccgca gatcctgcgg atcgtcgatg ctgacccgtc cgtatttccg aacggacacg
     1621 ccggacagca tgatcagccg gcacaccgca aacggtgtca gtcgagactc cacgaaaacc
     1681 acgattcgat agatttgact ttccattact acttcacttg ctgttccgac gacgactctc
     1741 gatgatgcga ggcgttcatc gtcgaagttt cttgccaact tgttcgcaaa atgttgtgca
     1801 gttcctcgtc ggatagctcg atcggattgc tgcgcatgct gcctttgcgc gattggctga
     1861 cgatctgcgc aaactgggcg ctttgaaccc cctgctcgct cagactgcga atccccaagc
     1921 gcgaacgcag aagcgacagc cgctcggcca aaagctccag atcggtctgc ccgtcggtca
     1981 gcagcgcaca cagctcgcgc attcgcacca gcgccacacc gtctggttgc cgtcgagaaa
     2041 gcgccttggc attggccaaa aacgtcgcag gcagaagaca cgcacagccg tcgccgtgtg
     2101 ccacgtcaca cagaccacca agcggcgcca ccaggccatg caccgcccca agtccggcat
     2161 tggcgagcac gattccgccc cacatgctgg ccagcgccaa atcatcgtgg acttccgacg
     2221 gcaacgccgc gccccccgca tcggtcgctg catacgattg tgcgagcctg tggagcgcgt
     2281 gccacagctt cccgaccccg cgcaggacca gtgcatcggt gatgggctgc gcgcctttgg
     2341 acagatagcc ttcgataaga tgggtcagcg catccatgcc cgcggctgcc gcgactcgct
     2401 gtggtgccgt cgctgacagt tccggatcga ccaccgcgat gcgtggcaag agctgatcgc
     2461 cgcgcaagct gcgcttcact ttcagatcgg gcactttgag cacgctgttg cgcgtgactt
     2521 ccgatccgct ccccgccgtc gtcggcatcg cgatcaccgg cagtggcgtc tgcacaatcg
     2581 gcagcggcgc gccgcgtccg atgtcttcca agtaatcgat cgcagtgcct gcattttgcg
     2641 aaagtgcagc caccgccttg ccgacgtcga ggacactgcc gccgccgatc gccagcacga
     2701 cctgacagcc ggactcgcga cactgccgga cgagtgcatc tgtagcggcc acatcgggct
     2761 cgcccgatac cgtgacgtgc tcgacgtgct ctgccgcgtc gcccagatca gccgcgatcc
     2821 gtgcaagcac accgttttgc gaaagcgacg aagcccccgt gaccagcagc acccgcttgc
     2881 ccaaacccac ggcaagctgg gcggcgcgac gatgttcacc gcgtccaaaa atgagctgcc
     2941 cggcggtacg aaactggaac tgcatcttcg cgggctgctt agtgaaaggg gctgggaaac
     3001 aagctgtggt gtttttcacc gacgcgcggg cttgccatca tgtcggccac cgtctgtcgc
     3061 cacagaagat agtgcggcgt ctgctgatgc gatttgaagc cggcctcgtc gtgatagacc
     3121 tcgtacagcg tgaagcgcgt cggatcttcg ttttgctgaa gcacatcgaa gcgcacattc
     3181 cccggttcct gccgggttgc gtgggcgttc gccagcgtcg cagaaacaaa gtcgctcacc
     3241 cgatccgcaa tgaccgatat ccgcacgacg acgacataca tgggcagctc cctttttgcg
     3301 gttacatcac gcgccagacc gactgaatcg gaccaaacgg aacccccaag cgcccaatca
     3361 ctggcaggtt cacaaacacg gtgccttccg ccgcgacggg aatctccagt gagttgcgct
     3421 gctgacggag caagttttcg acgattggct ggatgacgtt ctgaatcacc cgcagcgcct
     3481 gatcgatgcc gagcggaaag gccaagttga gatggacatc accgcccgcc gggatcagcg
     3541 acttgcgcag ctcgttcaca agcgatccat tggccaagcc aacgatcagt ccgggaatcg
     3601 ctcgcacaaa gtccgaagcg gttcggatgc cgccattggt cgccgtgcaa gcgcccggct
     3661 tcggctgtcc ggtacacgat ggatcgttgc caccgcacat gctcacgcag acggacccga
     3721 gcgcttcggc gttttgcccc ggaaacagct tcagcgcgag cagcacgtcg agcgccggca
     3781 ccggaatgtt gttcggattc ttgatcgtga ccggcaccgc aaattcaaac tggagctgct
     3841 gctggggcgg cggtgagcca agcccaatgg agcagcccat gcgcagcatg cgatccgtga
     3901 ccacctgcgg gcagtaatag gccgccagcg cttgcaacgt cggcgcccgc acaagctgtg
     3961 gactgcccgc cgtcaccacg gggggattcg cacgctgctg ctggatgcca ctgccgacgg
     4021 cgtcttgcac aagccggttg aacatcgtgc atcccgacga aaacaccgcg agcagcagca
     4081 gccacgaaga aaacacaacc ctggacaaac gcatcagcga actcctaacg gcccagcacg
     4141 ggcatgagag catgaccact tccatcttac tgggaaggct ggtggaaggg gaacagccag
     4201 cggtccagcg gtttcaagcc cggagctaaa ttagttattt aaattacata taaaatttcc
     4261 aataattacg actggaagca atgggcgaac gcagcgggtt ggaccgtgcg aagatcgtgc
     4321 gcagggggct ctgggcaggg tggcggaact tatgtgccgc tgtgcccctg tcgctgtgtt
     4381 tgggtgaagc gtatgcagcg gcgctcccag agctgagcgc aggtcagcag cagctccttt
     4441 tgtcgacggg cgaagagaaa ccgtacaatg tgcggctcca ctttctgaag tcgaacgagc
     4501 gccgccatga cctgtttttt tcggtgctca gtcggctcgg tggcggatat ctgggcgtcg
     4561 gagccgatca gaactacacg ctggctgctg tggccaacgc cgagctggtt ttcctggtcg
     4621 atatcgacgg agaagtcatt cgctggcaca aaatctatgc ggcgctgatt cccgtcgccc
     4681 cctcgcccca ggatctgctc aagctgctcc aagcgcgggc cgatgcgcag gcgaaagaag
     4741 cactggagca gcgctggccc accgaagctg cagagctttt gcagtcctat cgcaactacc
     4801 gccacatgct cggcatccac ctgcaaaacg agatgcaggt ccgtcacaac gggcgacctt
     4861 cgacgtggat atccgatccg gtgctgtatg caaggattcg tgcgctgatg atcgccaggc
     4921 gcgtcatcgc ccgcgtcggt gatctgcacg gagaacgtac catgctcagc attggcgacg
     4981 ctgcacacgc gctccacgtc accatccgca ccgtctatct gtccaatgtc gagcagtggt
     5041 ttggcagggt atatcggttg gcatgcgtgc cccacgttct accattttcc gtcggtaact
     5101 tccgttcatg tctgaaccgc tatacatcgg tcggactcga agttgtggac cacagttgga
     5161 ccacctgcaa gcgaggctgt gatgggtgac atcatcaaga agatgaaagg cgagaagttc
     5221 ctgggctggt acgtgcgcta cgtcgatgcc gatggctgcc gcaagcagcg cgcgagccat
     5281 cagccgacca agccgctggc tcggaagttt ctgatcgaga tcgaggcccg cgtcgcccgt
     5341 ggtcagatcg gcatccccga gccgtcgcca ccgccgcgcc ccgagctgac cctcgccgag
     5401 ctgtgtgagc gctacgtgca ggactacaac cgtccccgga tcaagaacct ggcgctctac
     5461 cggaccgaac agaaatcagc gctgaagcgc atcctgccga tcctcgggac caagccggtg
     5521 agcgcggtca cagcccatga cattcgacgg gttcgcgatc agctgtcgac gcgctacaag
     5581 ccgaatacgg tgatgtcgtc gctgcggcca ctgtctacgg cctttagctg gggcaagcgc
     5641 gaagggttga ttgactgtcc gaatccctgt ctcggcgtcg agcgaccgcg ccgagagagc
     5701 ctgcttgagt tcctcgacaa agacgagctg tcacgactgg tttcggcggc agaggcgaaa
     5761 gcacgcgcca gcgatacgtt tcgtgactgg ctgcgctatg tggcgatcgc gttcacggtt
     5821 cggactgggc tgcgcaaagg cgagctgttc ggtctgcgct ggcgggacct cgatctcacg
     5881 acgcgccggc tcgatgtggc gcggtcgtat cagcacacgc cgaagtcggg aaccacgcgg
     5941 catctgcgac tgcccagcca gctggtcccg attctcgcgg agtggaagag aatgtgtccc
     6001 gcgacgcccg atgacctcgt gttcccggtg atgggacgct ggagcctgcg gatgggtcgc
     6061 aagatcgaca tgctgggact gcccgagctc ttggcggaca tcggggtgcg cccgctccgt
     6121 cgcgagtggc acgcgctgcg ccataccttc gcgtcacact tcgtgatgtc gggcggcaac
     6181 atcctgacgc tgcaaaagat cctcggtcac gccgacacca agatgacgct cgtgtatgcc
     6241 catctcgcgc ccgatttcct cggcgatgag atggatcgga tcacgatcta gccggtgcgc
     6301 cgacgagaca tcggcaccgc gacccgcccg agatacccct cgatgtcgat gcgtcgaaag
     6361 taacggcgcc gctcgccctt gggcttcacc gccagctgcg ccacctctgg atggcgatca
     6421 agccaggtct gaaagcgccg cgccacttgg ttgcgaaggc tctgctcttt acgtcgccac
     6481 gcgcgatatt ccgccgttcc cgctggcggc tccggcggca gctcttggcg agggaatatg
     6541 ccgaggagct tggccgtctg ccacgtatca agtaacccct ccggctccga cgtgccctgc
     6601 tctcttcgtg cctgaccaag tccgagccga accgtcagca ggtccagtag gtccgcaacc
     6661 gtgagatcaa tcacacgggt atcgtcagag atgtccctaa gacgggccat agtaggccat
     6721 attttctaat tatttcgcgt gcttttcaat acatttcata aggcaagact tggatgtgat
     6781 tccacggatg tcgacgccag ttggggagtg gagaggacat gctggttctt gaacgtcgac
     6841 gcacaagttt caatggagcc ccagatagga atccggggtt ggaaatcggt tgatagggtg
     6901 gtctgagcga gggagtcacg gcgcgggtcc cccttcccga cgacgctttt tccgcgccgc
     6961 tcgcggcccg aaaaaaggtc tctggtcggc ccacgcctga tttttcaggg tcggaatccg
     7021 gggacggaac cccgcgatta ctgcttttga aggagcatca gcgcgagggt cttttttccg
     7081 tcggggccgt agtcgattcg ttcgatggtt ttccagccga gttcggcaag gctgtcactc
     7141 agcagcttgg ggtcgtagcg acggactcgc cacatcagga agcgacgatc gggcctaccg
     7201 tcgcgcatct tgacttgggc aatcgcatcg aaaccgtagc tgccgggaac gccgcccggt
     7261 ggggcaagct cccagcgaag ttcgatgtct tcagcaccga ggcagtagcg acgaatcggc
     7321 ccgctcagcc agttcacatg cgtaggtcgc agcttgccgt tgccaatcgg gtccagcttg
     7381 aggatcttct gccgttcact tgctggggca cgcgcaatct ggatatcaaa caggaacagc
     7441 tcgcccggct cgcagcagct cagcgtgtca cggaagaaac gcagctcatc gctcaggttg
     7501 accagggtgt atccgagcat ggcgaacagc cttgtccggc ctcgcagcgg attgaccagc
     7561 atgggcagct tcggcagatc cagaaagctg ccgtggatgg tgtacgtgcg gatgtcgggc
     7621 agcgtctcgc ggcagtgccg gtaggcggtg ttcagcagcg agtgactcac gtcgagcaag
     7681 aacagctccg tctgtttcgg catgcgaagc tgcatctgaa gatgcttgac gagcatcgac
     7741 tcggtcttgc cgtcgccgca gccgagcgca tccacggtga gtgcctgctc accaatgagg
     7801 tctgcgacgc ggcgcgcaac cgtcccgagc ggcagtgtct cgcgaaactc cgagccataa
     7861 tcgggcgacg agcaggtggc cagccagtcc ttcgcgctgt gatggtcgat gtagacaagc
     7921 gtctgctcaa gctgtccagt cggaccattc agcgtctcgg caagctccgt caccatgctt
     7981 accgggtcat acgtcgatcc gagccatgaa gtcggcgtgt gacggtagcc cgcgctcatc
     8041 tcggcggaca gctcgatgtc actgacgcgc aggttcagct cgggaacggc cagcagcttc
     8101 agcatcaagc tccgtctggc cctgcgggtg tgatgttcaa gctgacgaat cgtcgtccct
     8161 gtgacaccca ctcgatccgc cagccattcc tgcgtcagtc ctgctgcggt ccgccttttt
     8221 tgcagccgct cgccaaactt cacccagtcg atcggcccaa tcgcttcgct gtcgtcggtg
     8281 tcacccacca gcgtcagcgc ttcctgaatc gcgcgcaccg cccggttgtg gtccttcacc
     8341 agatgcggcg gcagaagctg aagcgcgtgc agcgtatcga cgagcttgct ctgcacccgc
     8401 gagagctgac cgacagagtc ttccagcgcc gagtcctgcg tcggcagctc ctgcggcatg
     8461 acgtgctccg catccctgtc caaacggtcg agttcgctgc tctgcgtcgc tggctgctcg
     8521 tgcatggctg atgtctccat ttcggccaca gataacacac cacgagcaaa tgggaagccc
     8581 tttcagcgac ggaaacgcag cggttccctt tgttcgtttc tgttcggtag cccggtgtgt
     8641 agcttcaccc aaacctggac tttaagaggc tcctaagatt gtaaggcggg ctcatctaca
     8701 gagcatcgtg gcgacggcga ttgctggcgc gtaggatgcg gaaacagaga gctgacgcga
     8761 tggaaaaacg aagctaccga agattcacag ccgaggagaa gcggacgatt ctggaagaga
     8821 ctcggcagcc tggggtgacg atggcggagg tgtgtcgcaa gcacagcgtc agtccggcgc
     8881 tgatctacca gtgatgggca gtggcgagcg gtggcgcagc aggcgacgac ggcggctttg
     8941 cagcaggtgg acagtcgagg caagagtcag cccgacgctc agacggcacg acttctggcc
     9001 gaactggagc ggatgcgccg cgtggtggcg gagatcacgg cggagaatct ggagctgaaa
     9061 aaaaactcta aagctggaag gaagagtccg tttgagcgcg cagcagaaac aggaagtgct
     9121 ggcggtggtc ga
//
LOCUS       DAORGN010000202         9126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3342_length_9126_cov_5.565318, whole genome shotgun sequence.
ACCESSION   DAORGN010000202 DAORGN010000000
VERSION     DAORGN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_9126_cov_5.565318"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="PKL17_17745"
     CDS             complement(<1..1085)
                     /locus_tag="PKL17_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46631.1"
                     /translation="MGRTRAGRYPQRLWAWLLPLLLIGLPALGAKPRKPPPAQLSPAE
                     KLTEQGLQALTEKDFSRAYAALSEAYRLAPTAESLLQLGRLAAAEQRPLEAQDLLRRY
                     LLDPARKPDDAMAQLAQRLLQEPSPPSGQVMVLAEPGALVSVDERLVGVLPLPTPLLL
                     SPGDHRLAVEYPNKRLSGQLTLAPGRQFEVRFDRSSGTLLLSLLPAVLVHSELSIVLP
                     DVRSDIQTTIEQSVRSLDQTVLPVEQALTVAPHLRDCMSKPNCQQLLAQKSDVEYVLH
                     ATLRRMDKGDGFVLRQVIRHRDVAEPAASLDSPCPKCSPADVVALMGRTLPTLLSGAL
                     KRRTGTLVVSTQPVSAEVMLGKSRLGAT"
     gene            1222..2088
                     /gene="xth"
                     /locus_tag="PKL17_17750"
     CDS             1222..2088
                     /gene="xth"
                     /locus_tag="PKL17_17750"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNK46632.1"
                     /translation="MAVKASSRLSPSAPTESGLDLSNCFSVATWNVNSLRMRWDRLCA
                     WLSDKRPDVLCLQEIKMQDADFPTLELHGLGYHAVAHGQKTYNGVAILSRVEHGSPEA
                     VKCGMQDGDPDAESRLIVATIPALGVRVASVYVPNGQVVGADKYEYKLRWLARFRSYL
                     DRECNPTQSLVLCGDYNIAPADSDVHDPEVWRDTVICHPSARDALGRILDFGLIDTLR
                     RCQPDGKIFTYWDYRGLSFPKNLGIRIDHILTTSVLADRCQKVEVDRQARKGEKPSDH
                     APVIAWFRREPK"
     gene            2160..2233
                     /locus_tag="PKL17_17755"
     tRNA            2160..2233
                     /locus_tag="PKL17_17755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2194..2196,aa:Met,seq:cat)
     gene            2303..2911
                     /gene="ruvC"
                     /locus_tag="PKL17_17760"
     CDS             2303..2911
                     /gene="ruvC"
                     /locus_tag="PKL17_17760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNK46633.1"
                     /translation="MTRILGVDPGTLHCGYAVVEVSAGPGAQKPRYIECGVIELPAQA
                     PIEHRLKLLAADLREVIRELRPDELAIECAFAGKNVRSALLLGQARGAILLVGAEANL
                     PCSEYPPATVKKTVCGNGRAQKSEVQRIVSWLCKLRTPPPTDAADAVAIALCHAFHAP
                     SRSISAQASDQAAAKRRPRATASRRTAQVRAPKAAPPARSDR"
     gene            complement(2793..3806)
                     /locus_tag="PKL17_17765"
     CDS             complement(2793..3806)
                     /locus_tag="PKL17_17765"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNK46634.1"
                     /translation="MTRVWGRVGLFFAATLALCAGYVTLSRDSGVAATRALGWGFPTV
                     MAHRGASYLAPEETEWAYRLARATGADFLEADIQRSKDGVLFALHDDTLERTTDAKTL
                     YPGREKLPVSEFTWDELSQLDAGSWFNAKHPERARPEYVGAKLLRLDRLIDLALASDR
                     APSGLYLETKSPERFPGIEADLVKLLAARGYAADPVPTVLQSRLVFQSFSADSLRNFQ
                     KLAPHIPRVYLLDEDMVKKTGFAELVAQAERLGRGIGPVGYYAYPWHNQRAHARGLVV
                     HPYTINSAWQLRLARFFGADGVFTDRCELAVPLLGRGPAQSVDSLWREAAASPLPDPM
                     PGH"
     gene            complement(3803..4366)
                     /locus_tag="PKL17_17770"
     CDS             complement(3803..4366)
                     /locus_tag="PKL17_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNK46635.1"
                     /translation="MAIHPSTASKHPVVRTAEHVAELKHQWSLYQRRNALKTTAQREL
                     IVDVFLSGNTGASHVSIDELLASVRLRNPKVGYATVYRTVKHLLDAGLAASRQFGDGQ
                     TRYEVAGSDSPHHDHLICQKCRLILEFEEPEIERLQDSVAARLGGFKVIQHKLELYGL
                     CPKEQGVPGGNCPFESDGQTDVGASGR"
     gene            complement(4384..6222)
                     /locus_tag="PKL17_17775"
     CDS             complement(4384..6222)
                     /locus_tag="PKL17_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNK46636.1"
                     /translation="MRILGLNAFHGDASAALLVDGKLVCAVEEERLNRIKHCAGFPTL
                     AVRECLRIGRISPSDIDHVAMSRDPRAHLFDKLLYVGQRLYQRRDVGRLWHQLRDRLG
                     NRRKVNSVEQALCVALGTESLAAQIHHVEHHRAHLASAFFASPFSESAVLSLDGFGDF
                     VSTMWGTGRDRQISVLGEVGFPHSLGALYTAVTQWLGFPKYGDEGKVMGLAAYGDAHA
                     VPALRRLIHLHPDGSFRLELAYFRHHQEGVDMSWADGQPRLGRLYSDALCELLGPAYQ
                     SGSAPDQRQKDVAASLQALLEDAVVHIALGLARRTGATRLSLAGGVALNSVANGKILD
                     RTPFRQLFVQPAAGDNGTSIGAALWVAHQLLAAPRRFVMHHAYTGPEFSDADCEQSLR
                     TVLGDGAQLDAQGQVVVDAVETVGRDGQKALGRLRIERLDTSDLLSRAVSAIERGDVI
                     GWFHGAMEFGPRALGHRSILCDPRRADMKDILNRRIKHREPFRPFAPAILADQTAEWF
                     VRAAPSPAMLLVDEVRAEKRALVPAIVHADGTGRLQTVEREHSPTFYALIETFFARTG
                     VPILLNTSFNEHEPIVCSPSDALRCFLKTHMDALALSNLWLSWVTD"
     gene            complement(6240..7790)
                     /locus_tag="PKL17_17780"
     CDS             complement(6240..7790)
                     /locus_tag="PKL17_17780"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46637.1"
                     /translation="MEAATLDEPARILLVDDEKVVREMLMHVLSAEGFSVRLAEDGLQ
                     AWQELQRRSYHLVLSDLKMPHMNGLELLQRISEARMQVVTIIMTAFGTVETAIEAMKR
                     GAYDYILKPFKNEDVIRIIQKGLKQQKLQQENFLLKEAVTLYQLSEAMAHELSLDRIL
                     DIILDATLKEVHADVATLTMRHPVTGEFVEKTRKYSKNAQTDLNWYGSATGVGELNTS
                     AILDHYMAGRPLLAHGIRALPFFAEPPKDKRLVSFCSTPLKIKDQVIGMLNAYSYSRG
                     YRFSEGQRRLLSVLASRAAVSIENARLYEQLQRSNADLTRANTSLRENFRATVVGFAH
                     ALEESDRYTRGHSERVSRYAELLGRRLTLSENELEMLRWAALMHDIGKIGIRQEKLNK
                     PGKLSPEEVAMFQTHPAKGKRILEPIPFMADLIPGAFCHHESWDGNGYPQSLMGENIP
                     LIGRIVAIADSYDAMTSDRAYRKAMPHETAISEIERCSGVQFDQRLVDVFLGAIDEFR
                     ESGQHLGH"
     gene            7858..8856
                     /locus_tag="PKL17_17785"
     CDS             7858..8856
                     /locus_tag="PKL17_17785"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK46638.1"
                     /translation="MPHSARRLRLCVVSSVGGHLREVLELVPSFGQADVRFIVNDVVN
                     VQLPGPVEQIAHAERDVRVLWNCVEAGRSFAKNRPDVLLSTGAGPIVPLASVGKLFGC
                     HTIYIETFGSVDTPSLTGRLMTRLADRIYYQWPQLASYFPTGQYAGPIFVPPRTTAAK
                     PNDDGSIFVAVGTSQRGFDRLLRWLDDLRQQGAVQIPIAAQRGHAHHIPQNYPSDPFL
                     PSQQLEARIAQAKLVICHAGAGLLGTCIRHGKRPIVVPRLARFGEAINDHQLMLAKAL
                     SASGQALLVQEKRELLPAIQAALAAPTIDTPEAKPTLCDAIAADLRRMASSRGCTV"
BASE COUNT         1859 a         3006 c         2537 g         1724 t
ORIGIN      
        1 gtcgcgccca gtcgggattt gccaagcatc acttcggcgg acaccggctg cgtcgacaca
       61 accagcgtgc ccgtccgtcg cttcagcgcc cctgacaaca gcgtcggcaa cgtccgcccc
      121 atcagcgcca ccacgtcagc gggtgaacac ttcggacacg gcgagtccag cgacgcagcc
      181 ggctcggcga catcccgatg ccgaatcacc tgccgcaaga caaagccatc gcctttgtcc
      241 atccgtcgca gcgtcgcgtg aaggacgtat tcgacatccg atttctgcgc caaaagctgc
      301 tgacagtttg gcttgctcat gcagtcgcgc aggtgcggcg ccaccgtcag agcttgctcg
      361 accggcagca ccgtctgatc gagcgagcgc accgactgct caattgtcgt ctgaatgtcc
      421 gatcgcacat ccgggagaac aatcgaaagc tcgctgtgaa ccagcaccgc aggcaaaagc
      481 gacaaaagca gcgttcccga cgagcggtca aagcgaacct cgaactgtcg acccggagcc
      541 agcgtcaatt gaccactcag ccgcttgttc ggatactcga cggccagacg atgatcccct
      601 ggcgacaaaa gcagcggcgt cggcaggggc agcacaccga ccagtcgttc atcgacggaa
      661 accagcgccc caggttcagc caaaaccatc acctgtcccg acggtggcga tggctcttgc
      721 aaaagacgct gcgccagttg tgccatggca tcgtcgggct tgcgcgccgg atcgagcaga
      781 tagcgtcgca ataggtcttg cgcttccagc ggtcgctgtt ctgccgccgc cagccgcccg
      841 agctgcagca gactttccgc cgtcggagcc agccgatacg cttccgacag cgccgcatag
      901 gcccgcgaaa agtccttttc cgtcagcgct tgcagtcctt gctcggtcag cttctcagcg
      961 ggcgacagct gcgcaggcgg aggtttgcgc ggcttggccc caagtgccgg aagcccgatc
     1021 agcagcagcg ggaggagcca cgcccaaaga cgctgcgggt agcggccagc gcgcgtccga
     1081 cccaagacgc gccccaaagg caagcagcga gagcactcgc acgcggttcc ccctcgccat
     1141 aacgcacgcc gcaaagacat ggctaggccg catagcttat cacgcagagc gagctacgtg
     1201 catgtgctac ggtgccgcgc catggctgtg aaagcatcgt cccgtttatc accttcggct
     1261 ccgacggaaa gcggtctcga cttgtcgaac tgtttttccg tcgccacctg gaacgtaaat
     1321 tcgctgcgca tgcgctggga ccggctgtgc gcgtggcttt ccgacaagcg ccccgacgtg
     1381 ctgtgcctgc aagaaatcaa gatgcaggat gccgacttcc cgacgctgga attgcacggt
     1441 ctgggctatc acgccgtcgc gcacggacag aaaacctaca acggcgtcgc cattttgagc
     1501 cgcgtcgagc acggcagtcc cgaagcggtc aagtgcggca tgcaagacgg cgaccccgat
     1561 gcggagtcac ggctgattgt cgcgacgatt ccggcgctcg gtgtgcgcgt cgcatcggtg
     1621 tatgtcccaa acggccaagt cgtcggggct gacaaatacg aatacaagct gcgctggctg
     1681 gctcgcttcc gcagttacct cgatcgcgaa tgcaacccga cgcagtcact cgtgctgtgt
     1741 ggcgattaca acattgcccc tgccgacagc gacgttcacg atcccgaagt ctggcgcgac
     1801 accgtgatct gtcatccatc cgcgcgtgac gcactcggac gcattctcga tttcggtctt
     1861 atcgacaccc tgcgccgctg tcagcccgac gggaaaattt tcacctactg ggattatcgc
     1921 ggcctctcct tcccaaaaaa cctcggcatc cgcatcgacc acatcttgac cacgtcagtg
     1981 cttgccgatc gctgccaaaa agtcgaagtc gatcgccaag cccgcaaagg tgaaaaaccg
     2041 tccgatcatg ctccggtcat cgcgtggttc cgacgagagc caaagtaatt tcacgatttt
     2101 cgaaaatagc cgttgacgcc ctgcgaaatt taagtagagt gctcctcacc tcaagttgat
     2161 gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcag aggttcaaat
     2221 cctctccccg ctactaaaaa cggaagccta gtgcttccgt ttttttttgc ccaaatggca
     2281 aggcgccgaa atgatgggct gcatgactcg cattctcggc gtcgatcccg gcacgctcca
     2341 ctgtggctat gccgtcgtcg aagtttccgc cggacccggt gcccaaaagc cgcgctacat
     2401 cgagtgcggt gtcatcgagc tgccggcgca ggcgccgatc gaacatcgac tcaagctgct
     2461 cgccgccgac ttgcgcgaag tgattcgcga gctacgcccc gacgagctgg ccattgagtg
     2521 cgcgttcgca ggaaaaaacg tccgctcggc acttttgctc ggacaagccc gcggcgccat
     2581 tctccttgtc ggcgcagaag ccaacctgcc ctgttccgaa tatccgcccg caaccgtcaa
     2641 aaaaaccgtc tgcggcaatg gccgagcgca aaaatccgaa gtccagcgca tcgtttcgtg
     2701 gctatgcaag ctccgcacgc caccgcccac cgacgctgcc gatgccgtcg cgattgcgct
     2761 ttgtcacgct ttccatgccc caagccgatc gatcagtgcc caggcatcgg atcaggcagc
     2821 ggcgaagcgg cggcctcgcg ccacagcgag tcgacggact gcgcaggtcc gcgccccaaa
     2881 agcggcaccg ccagctcgca gcgatcggta aacaccccat ccgcgccaaa aaagcgcgcc
     2941 agccgcagct gccacgccga attgatcgtg tagggatgaa cgacaagacc gcgtgcgtgc
     3001 gcccgttgat tgtgccacgg ataagcgtag tacccgacgg gaccgatgcc gcggccaagc
     3061 cgctcggcct gcgcgacaag ctccgcaaag ccggtctttt tcaccatgtc ttcgtcgagc
     3121 agatagactc tcggaatgtg tggtgccagc ttctgaaagt tgcgcaagct gtccgctgaa
     3181 aacgactgaa acaccagccg gctttgcagc accgtcggca ccggatccgc cgcatagccc
     3241 cgcgcagcca aaagcttgac cagatccgct tcgatgccgg gaaagcgctc gggcgacttg
     3301 gtttcgaggt acagcccgct cggtgcgcga tccgaagcca gcgcaaggtc aatcagccgg
     3361 tcgagcctca gcagcttggc accgacgtat tcgggtcgtg cccgctcggg atgcttggca
     3421 ttaaaccaac tccccgcgtc gagctgactc agctcgtccc acgtaaactc gctcaccggc
     3481 agtttttcgc gccccggata gagcgtcttg gcatccgtcg tccgttcgag cgtgtcgtcg
     3541 tgcagcgcaa acagcacccc gtctttgctg cgctggatgt ccgcttcgag aaagtccgcc
     3601 cccgtcgctc gcgccagccg atacgcccac tccgtttcct cgggtgccag gtaagaagcg
     3661 ccacgatggg ccatcaccgt cggaaatccc cacccgagcg cccgcgtcgc cgccaccccg
     3721 ctgtcacgcg acagcgtcac atagcctgca cacagcgcca acgtcgcagc gaaaaacagc
     3781 ccgacccgcc cccacacgcg cgtcatcgcc cagacgcccc cacatccgtc tgtccgtcgc
     3841 tttcgaacgg acaattgccg ccgggaacgc cctgctcttt tggacacagt ccatatagct
     3901 ccagcttgtg ctggatgact ttgaagccac cgagtcgcgc cgccaccgag tcttgtaggc
     3961 gctcaatctc tggctcctcg aactcgagga tcagacggca cttttgacag atcaggtggt
     4021 cgtgatgtgg cgagtccgaa cccgcgactt catagcgcgt ctgtccgtcg ccaaactgcc
     4081 tggatgcggc aagtccagcg tcgagaaggt gcttcaccgt gcgatacacc gtcgcgtagc
     4141 ccactttcgg attgcgcagt cggaccgaag ccaaaagctc gtcgatcgac acatgggaag
     4201 ctccggtgtt gccgctcaaa aagacgtcga cgataagctc cctttgggct gtcgttttta
     4261 acgcattccg acgctgatac aggctccact ggtgctttag ctccgccaca tgctcggcag
     4321 tgcgcaccac tggatgtttg ctcgcagtcg acggatgtat tgccactttg gactccgctt
     4381 cgatcaatca gtcacccagc ttagccacaa attcgacagc gcaagcgcat ccatgtgcgt
     4441 cttcaaaaaa cagcgcagcg cgtcactcgg ggaacagacg atcggttcgt gttcattgaa
     4501 cgaggtattg agcaaaattg gcacaccggt tcgagcaaaa aacgtctcaa tcaaggcgta
     4561 aaatgttgga ctgtgttcac gctcgacggt ctgaagtcgc cccgtaccat cggcatgcac
     4621 gatggccgga accagcgcgc gtttttcggc ccgaacttca tcaacaagga gcatcgctgg
     4681 cgacggggcg gctcgcacaa accactctgc cgtctgatca gccaaaatcg ccggcgcaaa
     4741 cggacggaag ggctcgcggt gtttgatccg tcgattcagg atgtctttca tgtccgcccg
     4801 tcgcggatca cacaaaatgc tgcgatgtcc gagcgcccgc gggccaaact ccatcgcacc
     4861 atggaaccag ccaatcacgt cgccgcgctc gatcgcagac actgcacgag atagcagatc
     4921 ggacgtatcc agacgctcga tgcgcagccg gcccaacgcc ttttgcccgt ctcgaccgac
     4981 ggtttccacc gcgtcgacca ccacttgtcc ttgcgcatca agctgcgccc catcgccaag
     5041 caccgtgcgc agcgactgct cgcagtccgc gtcggaaaat tccggtccgg tgtaggcatg
     5101 gtgcatcaca aagcggcgcg gcgcggccag cagctgatgc gcaacccaca gcgcagcccc
     5161 gatggatgtt ccgttgtccc cggcggcggg ctggacaaac agctgtcgaa acggcgtccg
     5221 atcgaggatt ttgccgttgg ccaccgaatt gagcgcaacc ccacccgcaa gtgatagtcg
     5281 cgtcgccccc gtccgtcgcg ccagcccaag tgcaatgtga accaccgcat cctccagcag
     5341 cgcctgaagg ctcgcggcga catctttctg tcgctggtcg ggtgcactgc ccgactggta
     5401 ggccgggcca agcagttcac acagcgcatc gctgtacagc cggcccagac gcggctgccc
     5461 gtcggcccac gacatatcga cgccttcttg gtggtggcga aaatacgcaa gctccagtct
     5521 aaacgagcca tctgggtgca ggtggatcag ccgacgcagc gcgggcaccg cgtgggcatc
     5581 gccataggcc gccagaccca tcactttgcc ttcgtcgccg tacttgggaa aacccagcca
     5641 ctgcgtcacc gcggtataca gcgcgccgag cgagtgcgga aaaccgactt ccccgagcac
     5701 gctgatctgg cgatcgcgac ctgtgcccca catcgtcgag acaaaatcgc caaaaccatc
     5761 gagcgagagc accgccgatt ccgaaaaagg cgaagcgaaa aacgcactcg ccagatgggc
     5821 acggtgatgc tcaacgtgat gaatctgtgc cgcaagcgac tcggttccca gcgcgacaca
     5881 gagcgcctgc tcgacggaat ttaccttgcg acggttgccc agccgatcac gaagctgatg
     5941 ccacaaccga ccgacatcac gacgctggta aagccgctgc ccgacgtaaa gcagcttgtc
     6001 aaacaggtgg gcgcggggat cgcgggacat cgcaacatgg tcaatgtccg acggggaaat
     6061 ccgcccaatc cgcaggcatt cccgcacggc cagcgtcgga aatcccgcac agtgcttgat
     6121 ccgattgagc cgttcctcct cgaccgcgca gaccagcttg ccgtcaacca gcagcgccgc
     6181 gctggcatcg ccatggaacg cattcagacc caaaattcgc atatcaaccc ggcaaccgtt
     6241 tagtgaccga ggtgctgccc gctttcgcgg aactcatcga tcgccccgag gaacacatcg
     6301 acgagccgct gatcaaactg cacccctgaa cagcgctcta tttccgatat cgccgtttca
     6361 tgcggcatcg ctttgcggta cgcacggtct gacgtcatcg catcgtaact gtccgcaatg
     6421 gcgacgatgc ggccaatcag cgggatgttt tcgcccatca gcgactgtgg gtagccgttg
     6481 ccgtcccacg actcgtgatg acagaacgca cccggaatca ggtcggccat aaacggaatc
     6541 ggttccagga tgcgcttgcc cttggccgga tgggtctgga acatcgcgac ttcttcgggg
     6601 ctgagcttgc ccggcttgtt gagtttttcc tgtcggatgc cgattttacc gatgtcgtgc
     6661 atcagcgccg cccagcgcag catctccagt tcattttccg acagggtcag ccgacgaccg
     6721 agaagctctg catagcgact gacccgctcg gaatggccgc gggtatagcg atcgctttct
     6781 tcgagcgcgt gggcaaaacc gacgacggtg gcgcgaaaat tttcccgcag cgacgtgttg
     6841 gcccgtgtca agtcggcgtt gctgcgctga agctgttcgt aaagccgcgc attttcgatc
     6901 gacaccgccg cacgcgacgc caacaccgac agcagccgcc tttgcccttc tgaaaagcga
     6961 tagccgcggc tgtaggaata ggcgtttagc atcccaatga cttggtcttt tatcttcagc
     7021 ggcgtcgagc aaaacgacac cagccgcttg tctttgggag gctcagcaaa aaacggcagc
     7081 gcgcgaatcc catgcgccaa aagaggccgc cctgccatgt agtggtccag gatcgcgctg
     7141 gtgttcagtt ccccaacccc cgtcgctgat ccgtaccagt tcagatccgt ctgcgcgttt
     7201 ttcgaatatt tgcgggtctt ttcgacaaac tcgccagtca ccgggtggcg catcgtcagc
     7261 gtcgccacat cggcatgcac ttcttttagc gtcgcgtcga gaatgatgtc caaaatgcgg
     7321 tcgagcgaca gctcatgcgc catcgcttcc gacagctgat acagcgtcac cgcttctttg
     7381 agcaggaagt tttcctgctg aagcttctgc tgcttgagcc ccttttggat gatgcgaatc
     7441 acatcctcgt ttttgaacgg cttcaggatg taatcgtaag cgccgcgctt catcgcttcg
     7501 atcgcggtct cgacggtgcc aaacgcggtc atgatgatcg tgaccacctg catccgcgct
     7561 tcggaaatcc gctggagcag ctcgaggccg ttcatgtgcg gcatcttcaa atccgacagc
     7621 accaagtgat acgaccgtcg ctgaagctct tgccaggctt gcagtccgtc ctcggccagc
     7681 cgcaccgaga aaccttccgc cgaaaggacg tgcatgagca tctcacgcac gaccttttca
     7741 tcatctacca acagtattcg cgctggctcg tcgagagtcg ccgcttccat cgcggggaat
     7801 ctactgccaa acacccaaag tccgcaacaa aaccgcttgg gctactatgc ctttgccatg
     7861 ccgcactctg cccgaaggct ccgtctctgc gtcgtcagtt ccgttggtgg acacctgcgc
     7921 gaagtgttgg agttagtccc atcgtttggg caggccgatg tccgcttcat tgtcaacgac
     7981 gtggtcaacg tgcagctgcc tggccccgtc gagcagatcg cccatgccga gcgagatgtg
     8041 cgggtcttat ggaactgtgt cgaagcaggc cgaagctttg cgaaaaaccg ccccgatgta
     8101 cttttgtcga cgggagcagg ccccattgta ccgcttgctt cagtcggaaa actgttcggc
     8161 tgtcacacga tctacatcga gacatttggt tccgtcgaca caccgtcgct cacgggccga
     8221 ctgatgaccc ggctcgccga tcgcatttac tatcagtggc cgcagctcgc ttcgtatttt
     8281 ccgacgggac agtacgcggg accgatcttt gtcccgccgc gaaccacagc cgccaaaccg
     8341 aatgacgacg gcagcatctt cgtcgccgtc ggaacatcgc agagaggctt cgatcgcttg
     8401 ctgcgctggc tggacgatct gcgccagcaa ggagcagttc agatcccgat cgcagcccag
     8461 cgtggccatg cgcaccacat tccccaaaat tacccaagcg atccgttttt acctagtcag
     8521 cagctcgaag cgcggatcgc ccaggccaag ctggtcatct gtcacgcggg cgcgggcctt
     8581 ttaggcacct gcatccgcca cggcaaacga cccattgtgg tgccgcgtct ggcccgcttt
     8641 ggcgaagcaa taaacgatca tcagctgatg ctggccaagg cgctttcagc gtcgggacag
     8701 gcgctgctcg tgcaggaaaa acgagagctt ctgcccgcaa tccaggcggc cttggcagcc
     8761 cccaccatcg acacgccgga agccaagccc acgctgtgcg atgccatcgc cgccgacttg
     8821 cgacggatgg ccagttcccg cggctgcacc gtctgatcgc ggcccgaaaa acgctgcgcc
     8881 ccgctgcgat aagtcttgga aatgggtggc aaaacccgcg cctagaccca ctctgattgc
     8941 gccataggct aagatcatgg cctagcacta ttcgcttttt tcacttacag cggcgatagg
     9001 agtcttcatc tatgcgttca acttggcttg gtctttcggt cggcattctc tcgctcatcg
     9061 gctgcaacgc gctgtggcag ggttatcttt caccgctcga cgaccccggt gacgcatccg
     9121 tcgcaa
//
LOCUS       DAORGN010000203         9043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3388_length_9043_cov_5.551847, whole genome shotgun sequence.
ACCESSION   DAORGN010000203 DAORGN010000000
VERSION     DAORGN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_9043_cov_5.551847"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKL17_17790"
     CDS             complement(<1..923)
                     /locus_tag="PKL17_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46639.1"
                     /translation="MIQKTPSHNTLSRRAFGLTLVAGLCAAVTAHAADGPLQLAFYAP
                     SAPLPSAEARYAFTEKLAQALQSSGLQVQTKIFAKPGDFEAAIKKGAVDLAILDAVYA
                     AERGNWNVLGIATASGDAYLRWGLYTHESSGNILDMAGKRLAWVAPGGKDPTYIGNVL
                     LYGELKASFFQLRPAAPDISAAVSEVVLRRADCVFAPEPAVSGKSLRKVYDAGEVGRI
                     PNPVLVQVSPRVTDAIAATVRKAAGSFNTTGVLDGFRTTAQAGEAIRSVRVRLRGRPE
                     RSLVLAEPSRLNTQVASTMVSAPELPPQSLPL"
     gene            complement(920..3718)
                     /locus_tag="PKL17_17795"
     CDS             complement(920..3718)
                     /locus_tag="PKL17_17795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46640.1"
                     /translation="MGQKMRALFSFRRWQGPALVLFAALGIGFSSPSAFAQGRAGSET
                     EEEAILKKAEEAFQNGDYDTAADLYDQAIRLDPNRVEAFVKRASLYFRQRKYTEAIDL
                     LTRAEKLSVSDLSVKTALGLTLYESGQKDRGLGYLEEVAKQRPETHGAQFQIGKHFAR
                     VNPQRAIAALEQYFRYRPEEDKRNDWEAQLLLGTAYYLTGKLTDSQKLLEQAQKSRPK
                     DNQIRQMLGTILIGQQQWAEGAAQYEPLQPDIQRRPAVAFNLATCYLHLGRRDEARKL
                     AQTYQTLKPDDPRGPILLAQIEQAGGKESDLREAIRRYQEAQELMQKKPEPSRVNVPA
                     AIGRAYLKLGDTAKAQATAEAALSELSGKPSSDALSVDEVQLLALALDSRIQQMNQAK
                     VPPGQGAPSLLSQAEKLAQLAPADAKVLALSGSGAYAAGNFEKARRYYSESQQIDDKV
                     VRSRIGLARTLEQLALLAIVTMSDPEPGKEGKEAKDPAKVAERQTAIGNAVSYLRQAQ
                     KLDDAPSIVRNLATALVMQGNGAEAEKLVLSAVQGSGKNDPLLWVLHARLLAQSKQFP
                     QAAEAADRAVSDARKQLDNLPAADGGRRPLLVRQLSLARIEQAARTVALDAARTGAAS
                     TKDRERLDTSIEALGQASRDLQGLPTGEGKELQKSVSRNLQLLHLRRGRLRLSDAETH
                     IARSGVSAQTTKEAEDALGDIQHAIELSPFEHKSELGFAQCLGALAGAQASQFKAARD
                     LVAKAKDNGCELVAPYNKLGTELVSVFVQYRASTAPLQREALLRTLPRLQGKATGNDA
                     ATLQKVLRALLYSTNLALAYDYHLLGRSKMVGPTLRNAQKVQPPRADDEDPVLSHNLA
                     VADLIEGKPVGEKVLERLAPNPPESLVNLGILYDRRGEIRKALDFYKRAAEKGARTAK
                     LREWIDTKDRMIGSGQSQ"
     gene            3875..6175
                     /locus_tag="PKL17_17800"
     CDS             3875..6175
                     /locus_tag="PKL17_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNK46641.1"
                     /translation="MMKREFPIRRPSPRAKLGTFGARSGLWLLLSASPTLAAGPEVHT
                     TAADGGRTAIDPKDDRVQRRAIRGGALDASRESAELRELRSFETEVFPRAGLRESLVR
                     PHEGSSLHGGSGPDGLPDGLHTPLPEKPETPPPSSSVPWLQALKLLDNLGLAPTFVRF
                     EAHVIKYLEFYKDERRGRSIMASWLRRQGRYKALIEQALDKYQLPRFLLYVSMIESGY
                     DPHDRSHKGAVGLWQFLPEGARIYGLRVDYWVDERQDPLRSTEAAARYLGDLKARFGS
                     WHLALAAFNAGYGAVLRAMQKYNTNDYWELCRHEDGLPWETLLYVPKAIATALVGENK
                     GFFGYDEMVPDPPLTFDSVTVHSSVSLAGAAKAIGATSEDVQRLNPHLRRGRTPPLGP
                     GETWELRLPTGTAAQFVAAYDYRGEKLEPYIVRFGERIEDIARVRAVAASRLRSINGI
                     EDATEVRAGLTLLVPPAKASFDPGQNGSLAGDEIVVVAVPDKNFSVPGKKQIFYRTIS
                     GDSIWSIARFFKVKDTDLLRWNNLDPEATLATKMVLSLWIDPQVDTSQVVLVDPLRVR
                     VVTTGSDEFFELVETLRGRRRLQIRVAAGDTLERIGKRFGLSAADMERINRMPRTTEL
                     QPGQTVIAYQNMSASERSALIRKLLAGESLDAPAVDSKDEKGGLAVYGPPAPLSDEKG
                     AYGPPHPGSVTAPSGDSSRADADGDVAFPKADTSTGSDAQPVPFGPPSPTKKPGTVPA
                     EKVKPTPSAEPLPSTSEPASLEDEVP"
     gene            6310..6870
                     /locus_tag="PKL17_17805"
     CDS             6310..6870
                     /locus_tag="PKL17_17805"
                     /inference="COORDINATES: protein motif:HMM:NF016503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERG2 family protein"
                     /protein_id="HNK46642.1"
                     /translation="MGYIFDPDKLHHLSQRVVGMPREQLIPQLVSDIAEAYPGYISTK
                     QNWIFNLAGGATGSMMVLHGSLTEYLIIFGSSIGTEGYSGRYRLDIHDYMLDGEMWVY
                     VGDRPTERITYKPGDHAKLPRGMTKAWKSPENAWMLEYGRGNVPSCLPVGLGGAIFSA
                     VEPKIVWDTVKEYGGQTIRNLLKGKF"
     gene            6900..8189
                     /locus_tag="PKL17_17810"
     CDS             6900..8189
                     /locus_tag="PKL17_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46643.1"
                     /translation="MSQKEVSSESQPHDPDAAKEKYGFGSSWLPIMLLLPPLVYYMWL
                     CMRDHGGAFYVPRSLDELVNLVKQVPAPTVTSVLVVAGWYGGQALLQVYAPGEWVEGS
                     PLADGTRLPYKMNGWFSFWLTILSLFFACWMGWLPATLLWDEFGPIMTTLNIGVYVFC
                     LYLYFKGKASPEKAYTGRVMYDYFMGTELNPRVGRFDFKLFCEARPGLTLWVLINFSI
                     AAKQWQIHHTVSTPMMLVCLFQFFYIMDYYFHEEAILTTWDIKHEKFGWMLCFGDLVW
                     VPFTYSLQAQYLLSHPNDLPTWATLAIIALNVAGYAVFRGTNIQKHHFSRHPERPIWG
                     KPAEFIQTARGTKLLHSGFWGIARHLNYCGDLMMALAWCLTCGVHNLLPYFYIIYFTW
                     LLVHRERRDDAFCHAKYGKDWETYRQKVPWRIFPGIY"
     gene            complement(8243..8962)
                     /locus_tag="PKL17_17815"
     CDS             complement(8243..8962)
                     /locus_tag="PKL17_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46644.1"
                     /translation="MKPATVRSPKWVEPCLAALTFAIFQGGCLPLPFVLPPVSVSAGI
                     GPGGGIMEAAPDLPAQPTGTQLSEEFRFVARPLAAVPALLNRPLDVGIGYKVQRLAAV
                     SSEPDAPASPWLHGPSVELRIFPWRQVSRQTALRLAIVTHVDLLGARPGQPFQASGGG
                     DVGLEFSLSHFLHGSPGAGGDKHAMVAGVAWGEIGIGAALTAGQEVIAGQHFGYGMLS
                     LTMQLPAAIGVALIPLTSLFR"
BASE COUNT         1646 a         2684 c         2921 g         1792 t
ORIGIN      
        1 agggggaggc tttgcggggg cagctcgggg gcggacacca tggtggatgc gacctgggtg
       61 ttgagtcgcg agggctcggc gaggacgagg ctgcgttcgg ggcgaccgcg caggcggacg
      121 cggactgagc ggatggcttc gccggcctgg gcggtggtgc ggaagccgtc gagcacgccg
      181 gtggtgttga agctgccggc ggctttgcgc acggtggcgg cgatggcgtc ggttacgcgc
      241 ggcgagacct ggaccagcac cgggtttggg atgcgaccga cctcgccggc gtcgtagacc
      301 ttgcgcaggg acttgccact gacggcgggc tcgggggcaa acacgcagtc ggcgcggcgc
      361 aagacgactt cgctgacggc ggcagagatg tccggggccg cggggcgaag ctggaaaaac
      421 gacgccttga gctcgccgta cagaaggacg ttgccgatgt atgtggggtc tttgccaccg
      481 ggggcgaccc aggcgagccg cttgccggcc atgtcgagga tgttgccgct cgattcgtgg
      541 gtgtaaagcc cccagcgcag gtaggcgtcg cccgacgcgg tggcaattcc gagcacgttc
      601 cagttgccgc gctcggcggc gtagaccgca tcgaggatgg ccagatcgac ggcgcccttt
      661 ttgatcgcgg cttcgaagtc gccgggcttg gcaaaaatct tggtctggac ttgcaaaccg
      721 gacgactgga gcgcttgggc cagcttttcg gtaaacgcat agcgcgcttc cgctgaaggc
      781 aggggtgccg acggggcata aaacgcaagc tggagcggtc cgtcggcagc atgcgcggtc
      841 acagccgcac acagcccggc cacaagggtc aaaccgaacg cgcgacgaga cagggtgttg
      901 tgggacggcg tcttttggat cattgcgatt gccccgatcc gatcatgcgg tctttggtgt
      961 cgatccactc gcgcagcttg gcggtgcggg cgcctttttc ggcggcgcgt ttgtaaaagt
     1021 cgagcgcctt gcgaatttcg ccgcgacggt cgtacaaaat gcccaggttg acgagcgatt
     1081 cgggcggatt gggcgccaga cgttccagca ctttttcgcc gacgggcttg ccttcgatga
     1141 ggtcagcgac cgccaagttg tggctgagca ccggatcttc gtcgtcggcg cgcggcggct
     1201 ggactttttg ggcgttgcgc agcgtcgggc cgaccatctt gctgcgtccc agaaggtgat
     1261 agtcatacgc cagcgccagg ttggtcgaat acagcagggc gcgcagcact ttttgcagcg
     1321 ttgcggcgtc gttgccggtc gctttgcctt gcaggcgtgg cagcgtgcgc aaaagtgctt
     1381 ccctctggag cggcgccgtc gacgcccggt actgcacaaa caccgataca agctcggttc
     1441 ccagcttgtt atacggcgcg accagctcac agccgttgtc tttggccttg gcgacaaggt
     1501 cgcgcgccgc cttaaactgg ctggcctgcg ccccggcgag cgcacccagg cactgggcaa
     1561 agccaagctc ggacttgtgc tcgaacgggg acagctcgat ggcgtgctgg atgtcgccca
     1621 gcgcatcttc ggcctctttg gtcgtctgcg cgctgacgcc gcttctggcg atgtgggttt
     1681 ctgcatcgga aagccgcagc cggccacgtc gcaggtgcaa aagctggaga ttgcgcgaca
     1741 ccgatttttg cagttctttg ccctcgcccg ttggcagacc ctgaagatcg cgacttgcct
     1801 ggcccagcgc ttcgatgctg gtgtcgagcc gttcgcgatc cttggtcgat gctgccccgg
     1861 ttcgcgccgc atcgagcgcc accgtccgcg ccgcttgttc aatgcgcgca agcgagagct
     1921 ggcgcacaag cagtggccgt cgacccccat cggcggcggg caggttgtcg agctgtttgc
     1981 gggcatcgct cactgcgcgg tcggcggctt cggcggcctg gggaaactgc ttcgactgcg
     2041 ccagaagccg cgcgtgcagg acccacagca gcggatcatt tttgccactg ccttgtaccg
     2101 ccgataaaac cagcttttcg gcttcggcac cgttgccctg catgaccagc gccgtcgcca
     2161 agttgcgcac gatcgacggt gcatcatcga gcttttgcgc ctggcgaaga tacgacaccg
     2221 cattgccgat ggcggtctgt cgctcggcaa ccttggcggg atctttggcc tctttgcctt
     2281 ctttgccggg ctctggatcg ctcatcgtca caatcgccag cagcgccagc tgttccagcg
     2341 tccgggccag accgatgcgt gagcgcacca ctttgtcgtc aatctgctgc gattcgctgt
     2401 aatagcgacg ggctttttcg aaattgcccg ctgcataggc cccacttccc gacagcgcca
     2461 gcactttggc atccgcgggg gccagctgcg ccagcttttc ggcttggctc agcagcgacg
     2521 gggcgccttg acctggcggc accttggctt ggttcatctg ctgaatccgc gaatcgagcg
     2581 ccagggccaa aagctgcact tcatcgacgc tgagcgcatc ggacgacggc ttgcccgata
     2641 gctcgctgag cgccgcttct gccgtcgcct gcgcttttgc ggtatcgccg agcttgagat
     2701 acgcccggcc aatcgccgct gggacgttga cgcggcttgg ctcgggtttt ttctgcatca
     2761 gctcttgcgc ttcttggtag cgacggatgg cttcgcgcag atcggactct ttgccaccgg
     2821 cctgttcaat ctgtgcaagc agaatcgggc cgcgcggatc gtcgggcttt agcgtctgat
     2881 acgtctgcgc cagcttgcgg gcttcgtcgc ggcgtcccag gtgcaggtaa caggtcgcca
     2941 ggttgaacgc gaccgccgga cgccgttgga tgtcgggctg gagcggctcg tactgcgccg
     3001 cgccctcggc ccactgctgc tgcccgatca ggatcgtccc cagcatctgc cggatctggt
     3061 tgtctttggg ccgtgacttt tgcgcttgtt cgagaagttt ttgcgagtcg gtcagttttc
     3121 ccgtcaggta atacgccgtc ccgagcaaaa gctgcgcttc ccagtcgttg cgcttgtctt
     3181 cctcggggcg atagcgaaag tactgttcca gggcggctat cgcgcgctgc ggatttacgc
     3241 gggcaaagtg cttgccgatt tggaactgcg cgccgtgcgt ttccggtcgc tgcttggcca
     3301 cttcttcgag atagccaagt ccccgatctt tctgtccgct ttcgtacagc gtgagcccga
     3361 gcgcggtctt gaccgatagg tcgctcaccg acagcttttc ggcgcgggtg agcaggtcga
     3421 tggcttcggt gtatttgcgc tgacggaaat acagcgacgc tcgcttgacg aacgcttcga
     3481 cgcgattggg gtcgagccgg atggcctggt catacagatc ggcggcggtg tcatagtcgc
     3541 cattctgaaa ggcttcctcg gcttttttca ggatggcttc ttcctcggtt tcgctgccgg
     3601 cgcggccttg cgcaaacgcc gagggcgaag aaaaaccgat accgagggcg gcgaaaagga
     3661 ccagtgccgg cccctgccat ctgcgaaacg aaaacagcgc acgcatcttt tgccccatgg
     3721 gtcaaaacgt agtgagtaat tgatgatact cgctagcccc ctcgcgaggg aactactaga
     3781 gcccgccatc gcacgcgcga ctcgcggtgg cccctgaaag ccgggcgacg caaaaatgtt
     3841 ggtggcggcc aatgtgtgct gctaacttcc caaaatgatg aagcgcgagt tcccgatccg
     3901 ccggccaagc cccagggcca agctgggcac gtttggggcg cggagtgggc tttggctttt
     3961 gctttccgca agtccgacac tcgctgcggg tccagaggtc cacaccacgg ctgcggatgg
     4021 cggtcgcacg gcgattgatc ccaaagacga ccgggtgcaa aggcgcgcga ttcgcggtgg
     4081 cgcgctcgat gcgtcgcggg aatctgccga gctgcgcgag cttcgatcgt ttgaaaccga
     4141 ggtctttccg cgcgccggtc tgcgcgagag cctggtgcga ccacacgagg ggagcagcct
     4201 gcacggggga tcgggaccgg acgggctgcc cgacggactg catacgccgc tgccggaaaa
     4261 gccggaaacg ccaccgccca gctcgtcggt gccgtggcta caagcgctga agctgctgga
     4321 caacttgggg ctggcgccga cgtttgtgcg cttcgaggcc catgtcatca aatacctgga
     4381 gttttacaaa gacgagcggc gcggtcgatc gatcatggcg tcgtggctgc gtcggcaagg
     4441 tcggtacaaa gcgctgattg agcaagcgct cgacaaatac cagctgccgc gcttccttct
     4501 gtatgtgtcg atgatcgaga gcggctacga cccgcacgat cgatcgcaca agggcgccgt
     4561 cggactttgg cagtttttgc ccgaaggcgc gcggatctac ggcctgcgcg tcgactactg
     4621 ggtggatgaa cggcaagatc cgctgcgatc gacggaagcg gcggcgcgct acctgggtga
     4681 tttgaaagcg cggtttggat cgtggcactt ggctttggcg gcgtttaacg cgggctacgg
     4741 cgcggtgctg cgggcgatgc aaaagtacaa caccaacgac tactgggagc tgtgccggca
     4801 tgaagacggg ctgccgtggg aaacgctcct gtatgtgccc aaagcaattg cgacggcgct
     4861 cgtcggggaa aacaaaggct tctttggcta cgacgagatg gtgcccgatc cgccgctgac
     4921 gtttgattcg gtgacggtgc acagctcggt gagtctggcc ggcgcggcga aagcaattgg
     4981 ggcgaccagc gaagatgtgc agcggctgaa tccgcatttg cgacgcggac ggacgccgcc
     5041 gctcggaccg ggggaaacct gggaactgcg cttgccgacg ggtactgcgg cgcagtttgt
     5101 ggccgcttac gactatcgcg gcgaaaaact agagccgtac atcgtgcggt ttggcgagcg
     5161 gatcgaagac atcgcccgcg tccgtgccgt cgctgcgagc cggctgcgca gcataaatgg
     5221 catcgaagat gccaccgaag tgcgcgctgg actgacgctg cttgtgccgc ctgcgaaagc
     5281 gagctttgat ccggggcaaa acggctcgct ggccggcgat gagatcgtcg tggtcgccgt
     5341 ccccgacaaa aacttcagcg tccccggcaa aaaacagatt ttttaccgca cgatttccgg
     5401 cgacagcatt tggtcgatcg cacgcttttt taaagtcaaa gacaccgacc ttttgcgctg
     5461 gaacaacctc gatccggaag cgacgctggc gaccaagatg gtgctgtcgc tgtggatcga
     5521 tccgcaggtc gatacatcgc aggtggtgtt ggtcgatccc ttgcgggtgc gggtggtgac
     5581 gacgggcagc gacgagtttt ttgaactggt cgagacgctg cgggggcgac ggcggctgca
     5641 gattcgggtc gcagccggcg acacgctgga acggattggc aagcggtttg gactgtcggc
     5701 agcggacatg gagcggatca atcgcatgcc gcgaacgacg gaacttcagc cgggccagac
     5761 ggtgattgcc tatcaaaata tgtcggcgtc ggaacgcagc gcattgattc gcaagctgct
     5821 cgcgggggaa tcgctggatg cgccggctgt cgatagcaaa gacgaaaagg gcggtctggc
     5881 agtgtatggg ccacctgcgc cgctatccga tgaaaagggc gcgtatggac cgccccatcc
     5941 gggcagcgtg actgcgccat cgggagattc atcccgcgcc gatgccgacg gagatgtcgc
     6001 ttttcccaaa gcggatacat cgaccggcag cgatgcccag ccggtgccgt ttggtccgcc
     6061 cagtccgacg aaaaaaccgg ggacggtgcc tgccgaaaaa gtgaagccga cgcccagcgc
     6121 cgaaccgttg ccgtcgacaa gtgagccggc atcgctggaa gacgaggttc cctaaacggc
     6181 ctgtgatgtt ggcagtttgg cccgtccgat ccgtcggtcc cgtgcgatag cgaacgtaga
     6241 caagccagct tcgctttggc aaagttccgc gcggcaattg cgccaagcaa cgttcgcagg
     6301 gacacctcta tgggctacat ctttgatccg gacaagcttc accacctgtc tcagcgcgtc
     6361 gtcggtatgc cgcgtgagca gcttatcccc cagctcgttt ccgacattgc cgaggcgtat
     6421 cccggctaca tatcgaccaa acagaactgg atcttcaacc tggccggcgg tgcaaccggc
     6481 agcatgatgg tgctgcacgg atcgctgacg gaatacctga tcatctttgg atcgtcgatt
     6541 ggcaccgaag gctattcggg acgctatcgg ctcgatatcc atgactacat gctcgacggc
     6601 gaaatgtggg tctacgttgg cgacaggccg accgagcgga tcacgtacaa gccgggcgat
     6661 cacgcgaagc tgccgcgcgg catgaccaaa gcgtggaaaa gtcccgaaaa cgcctggatg
     6721 ctcgaatacg gtcgcggcaa cgttccaagc tgtctgcccg tcggtctggg cggtgcaata
     6781 tttagcgcag tcgagcccaa gattgtctgg gacaccgtca aggaatacgg cggacagacc
     6841 attcgcaacc tgctcaaagg caagttctag cgctgcgttt gtcagcagaa ggacggctta
     6901 tgtcgcaaaa ggaagtcagt tccgaatccc agcctcacga tccggatgcc gccaaagaaa
     6961 agtacggctt tggatcgtcg tggctgccga tcatgctgct tttgccgcca cttgtttatt
     7021 acatgtggct ttgcatgcgc gaccacggcg gcgcgttcta tgttccacgg tcgctggacg
     7081 agcttgtgaa tttggtaaaa caagtgcctg caccgacggt gacttcggtg ctggtggttg
     7141 cgggctggta cggtggacaa gcgcttttgc aggtgtatgc gcccggtgaa tgggtcgaag
     7201 gatcgccgct cgcggacgga acccggctgc cttacaaaat gaatggctgg ttttcgttct
     7261 ggctgaccat cctgagcctg tttttcgcct gctggatggg ctggctgccg gcgacgctgc
     7321 tgtgggacga gtttgggccg atcatgacga cgctgaacat tggggtctac gtgttctgcc
     7381 tgtacctgta tttcaaaggc aaagcgtccc ccgaaaaggc ctatacgggc cgcgtcatgt
     7441 acgactactt catgggcacc gagctgaatc cgcgggtcgg gcgtttcgac ttcaagctgt
     7501 tctgtgaagc gcgtccgggc ctgactttgt gggtgctcat caacttttcg atcgcggcca
     7561 agcagtggca gatccatcac acggtgtcga cgccgatgat gctggtctgt ctgttccagt
     7621 ttttctacat catggattac tacttccacg aagaagcgat cctgacgacc tgggacatca
     7681 agcacgaaaa gtttggctgg atgctgtgct ttggcgacct cgtgtgggtg ccgtttacgt
     7741 acagcttgca agcgcagtat ctgctaagcc acccaaacga tctgccgacg tgggcgacgc
     7801 tcgcgatcat cgcgctcaac gtggctgggt atgcggtgtt tcgtggcacc aacatccaaa
     7861 agcatcactt tagccgtcac cccgaacgtc cgatttgggg caagcccgcc gagtttatcc
     7921 agacggcgcg cggtaccaag ctgctccact cggggttttg gggaattgcg cgtcacctaa
     7981 actactgcgg tgacttgatg atggcgctcg cgtggtgtct gacctgcggc gtgcacaacc
     8041 ttctgccgta cttttacatc atctacttta cgtggctgct cgtgcatcgc gagcgacggg
     8101 acgacgcctt ctgccacgcc aagtacggca aagactggga aacgtatcgc caaaaagtcc
     8161 cctggcgaat cttccccggc atctactagc ctaacctctt cccgaccgct cccgttttcc
     8221 ccaccgaccg caagccttgc tgttagcgaa acagggaggt cagcgggatg agcgccacgc
     8281 caatcgctgc gggtagctgc atggtcagcg acagcattcc gtagccaaag tgctggccgg
     8341 caatcacttc ttggcccgcg gtcagggctg cgccaatgcc gatctctccc cacgcgacgc
     8401 cagccaccat ggcatgtttg tctccgccgg cgccgggact gccatgtaga aagtgactca
     8461 gagaaaactc caggcccacg tcgccaccgc ccgacgcttg gaatggctgg cctggacgtg
     8521 ccccaagcag atcaacgtgg gtgacaatcg cgagccgcag cgcggtctgt ctcgatacct
     8581 gtcgccacgg aaagatgcgc agctcgacgc tgggtccgtg cagccagggc gaagcaggtg
     8641 catcgggttc agaagacaca gccgcaaggc gctgtacttt gtagccaatc cctacgtcga
     8701 gcggacgatt caaaagcgca ggaacagcgg cgaggggtcg cgctacgaag cgaaactcct
     8761 cgctaagctg ggttccggtc ggctgggcgg gtagatccgg agcggcttcc atgatgcctc
     8821 cgcctggccc aattccagcc gaaaccgaga cgggtggcag cacaaacggc agcggcaaac
     8881 agccaccctg gaaaatcgca aacgtcagcg cggccaagca cggctcgacc cacttgggag
     8941 agcggacagt cgcgggtttc atacccaaac acgataacgc aggctgcgga ccctgtatcg
     9001 gcccgcggcc ccgtgtcgtt ccgccgtcga ttagtagaag cgg
//
LOCUS       DAORGN010000204         9011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3408_length_9011_cov_5.605962, whole genome shotgun sequence.
ACCESSION   DAORGN010000204 DAORGN010000000
VERSION     DAORGN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_9011_cov_5.605962"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKL17_17820"
     CDS             complement(<1..679)
                     /locus_tag="PKL17_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNK46645.1"
                     /translation="MADSIREPLPYKVKDISLAEWGRKEITIAESEMPGLMALRAEYG
                     PSQPLKGARIAGCLHMTVQTAVLIETLTALGAEVTWTSCNIFSTQDHAAAAIAATGVP
                     VFAWKGESEEEYVDCIEMQLRAFKGGKAPNMILDDGGDLTWIIHEKHPHMLTGPDGIR
                     GLSEETTTGVHRLYEAHKSGKLKVPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDV
                     MVAGKIAV"
     gene            complement(738..5768)
                     /locus_tag="PKL17_17825"
     CDS             complement(738..5768)
                     /locus_tag="PKL17_17825"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNK46646.1"
                     /translation="MSERSTSPSIIPLRQTLVRRVLQVLGLIAFVWLGVFLGLNDRRL
                     GKLVTNIVGTQIRGEFRLGYARYSYFSSLASILLNTTTHVEGGDFEMRDPNGELVMKA
                     SRIEADIYLGELIRGLIRTAVSSPLNRGSFVELHFSSGHLHSAWAHIRPIQVRRLYPP
                     NVTGPLYGNEVNIVATMSGKKPKPEGAPPGPGHMRITIDGEGLTFENVTYQMSFPGWH
                     GQVDKLKGAVTLRYSSDAAETKPGLQSFVYEVAPMTADGGELVLGTREGAGEFVFPLK
                     NLELRRFGARASRKQDLVFRGKVQAADADVEVDGKLLDTYCDTGVSLDMSFTSGNGLA
                     KLVPGGLLSGQPRGRVRFFGALSSTLPTTISGRLSPCLSQDFHVQHFPLEGDGRSIVI
                     DGQVADVDAQIASIAVSEARSSFTLKGSDLHMPRVIASALGGQIRAEPLHFNFSGDIP
                     WYARITAIGTDPAQVGIMPKILQPIVAGRLKGTMKLSGSLAKNAHPERVVIDRVDAQV
                     ERLARRDPLPKDMKLLGSFVYTPDSVLFRGLSLSGDTLSLRSDHGAVSPRDGKLDIPV
                     MEMAGRGAPLGRVLQYFGVTGAAQEAQTRFRLGGRILRPEASRGEFAVKDLDLFGRKI
                     DALTTEFALHDGKLNLASLDAHGKDGSVSGDASIQLFAKDVSQRPDDPQLTVKAEVKK
                     VSVGLLLPGQDVSGLLTGKIDVTGTMQRPVGEVDLQVPSLKVLGGQFDDVSVLAQLSQ
                     TQAWLKSLSAKLSTGKLSSTALLKHERGRPLELAVTFDKLPIGQMPVVQGLPLVVDGI
                     LSGNVKMMGSLQPLLPVFDGKLSVSGFTVQGKLPTASGPVIEGGIVLAEREPIFGLLG
                     MVVRALTLGQAELQFSPSGSGTKITGRLFDSFDVDGVLSLLGGLPRGDLTIRFGCPLA
                     PYFVKTADSATGPRPTAAVLACDLLAQKLLPDLAQLGDVSVTGSGELRLRFGEDPRPL
                     FAPDPVFARGISCPMLASRPPVEEELLRFGATLRLSRAQLAVKTVTDEGEDQRYLAFN
                     DGEVFSCFDGRDIELGQARFVSQRRVGESSTEAGAVRFAGLISPVASDLRVRGQVRLE
                     LLEHLLRSVFRRTQGDAIVDVRITGPLANLSPQGTLELSRALVEPHELDTPIEVQGGK
                     LLLRPQRADLENVKIVVDSAVTLVRGGVEIDRYTPLQLGKIAFELRGDISARLLQWQF
                     ARNLAEARGRFGTDLLKVFGTFEEPIVEGTLTAHEVFLNLRRFHELSFSRGTIRFLRV
                     GGGDHGRIIIGRGEGSPSGVALSGLVDGDGKIELSGRVEHDGIGSFIKTPWHRALERV
                     DLAIKMENVRHSSSGVYNVEMSSAKGLTLTGSRDGISVGGDIEVVAGRYMQDYDPADR
                     FLSARRIVEEDKPFWDGDDFLSQIRLGLQVRTRGTFRVFNNIADLRLATQAFDVTGPL
                     SDIAMNGVIRVESGVFYVPGLRGEFHVKGDSKIVFSKTARWPDTPYVEVLGGTRDFDQ
                     NDQQRNVEVALRGRVRELKFECLASDGLSAADCASFLLLGDAADAVRGGKPNPTAGSP
                     TGARAIDYGDPAAKLVTSQLLTNQVADPLREKLRLDTVRIQFGVSTFDLQLCKRFGLY
                     IRMCGLAEWGLLGNAAARYRGYGELQMSDLTVGQISLERIERGFSFLEDTINRFKVQA
                     GLRLPLRY"
     gene            5841..6416
                     /locus_tag="PKL17_17830"
     CDS             5841..6416
                     /locus_tag="PKL17_17830"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNK46647.1"
                     /translation="MARVLKGGLRDGKKAHIDPYPFDPRAEAEKILSEAKHEAERLRN
                     QAITEGRERGLAAVTELLTAARVQTLRAHKDVEEDLRVLAVRIAERILGRELGQNPDA
                     VVDIVSEALSQAGAPREVVVRCHPDDLKALERGRPRLIERCSRTQAVLFRADATIARG
                     GCIVETELGTVDARLPTQLEAIERALRGQGP"
     gene            6413..7852
                     /locus_tag="PKL17_17835"
     CDS             6413..7852
                     /locus_tag="PKL17_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNK46648.1"
                     /translation="MSEPQGRSDRKDRADRPDPLRLSAALRTVQLTVPQRISGRVSEL
                     TGLVLRATAPGARIGELVAIDRFNEPIDDNRHSASLPHWRRGGTGETPLYAEVVGFRG
                     EEVVLLPLGSVAGVGPSSIVRPTGMPLTILTGTPLLGRVVDGYGEAIDDGPPLSSMLS
                     ELLPTPVEREAPEPLRRRRITEPLSFGLRAIDGCLTVGQGQRLGLFAGPGLGKSTLLG
                     QIARNASSDVTVIGLIGERGREVRDFIDRELDDETRRRTVVVCATSDQPPLLRLKAAL
                     SATAIAEHFRDRGKKVLLLVDSLTRVARAQREVGLAAGELPARQGYPPSVFALLPRLV
                     ERTGQGEFGSITAVYAVLVTGSGDDVDDIIAEEVRATLDGHIELSRSLAERGHYPAID
                     VLRSLSRVMDEICDATQRGAAAKLRELLSLSERQRDLVLLGAYQRGSDPATDEALQKL
                     PAIEEFLRQGRYERSDLAETRKRLTSLVR"
     gene            complement(7841..>9011)
                     /locus_tag="PKL17_17840"
     CDS             complement(7841..>9011)
                     /locus_tag="PKL17_17840"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNK46649.1"
                     /translation="VRWGDLVTQQKTRHAEFEVTDDKAVHVVAKDSGGAVIADVFIGK
                     ASGSATMVRVAGKDDVWQAIDLYSSTYKRESKTWREHSIYDLKADDSEKITIQGAGTK
                     VTLVREPVPAGADGKPGSQNIYEAKWKITEGDATLTPSLAVDNSLLNRMVQSVSTLRA
                     SDFHDAAKPEDVGLAAGAAGQIVVSVGYKEGKSAGVRIGAVKGEDTFVQTVDSAQIFT
                     VKKYAIESIAHIPQDVREKSLLSMKADQIDAVTIQQGTDVVSLKLVDKTWKADKLPDA
                     DEAKIKQIVEGFDGLAGTGFVAPDAPELKSLVPGKATITVKPKTGAPIVLKVGDARGE
                     DLAVQKAGSDAMWVRKMQLDRLLKKPADLAKDKNPPPAAPPPGPGGMPFPGGLPN"
BASE COUNT         1700 a         2880 c         2744 g         1687 t
ORIGIN      
        1 cgacggcgat cttgccggcg accatgacgt cggtggcgcg cttgatgccg tccacgagcg
       61 actcgcggca gccgtagagg ttgtcgaact tgctcttggt caccgagtcg ttgacgttga
      121 tcgccggcac cttgagcttg ccgctcttgt gggcttcgta cagacggtgc acgccggtcg
      181 tggtttcttc cgacagacca cgaatgccgt cggggccggt cagcatgtgc gggtgctttt
      241 cgtggatgat ccaggtcaga tcgccaccgt cgtcgaggat catgttcggg gctttgccgc
      301 ctttgaaagc acgaagctgc atctcaatgc agtcgacgta ctcttcttcg ctttcgccct
      361 tccatgcgaa caccggcaca cccgtcgcgg cgatcgcagc ggctgcgtga tcctgcgtcg
      421 aaaaaatgtt gcacgaagtc caggtcactt cggccccgag cgcggtcagc gtttcgatga
      481 gcacggcggt ctgaacggtc atgtgcaggc agccggcgat gcgcgcacct ttgagcggct
      541 gcgacgggcc gtattcggca cgcaacgcca tcaggcccgg catttcactc tcggcgatgg
      601 tgatttcttt gcgaccccac tcggcgagcg agatgtcttt gaccttgtac ggaagcggtt
      661 cacgaattga gtcggccatt aagagctcct tatgcggcgc acagggtagg cgccgggcgg
      721 tgtgtggtca agcgggccta atagcgcagg ggcagacgca ggccggcttg gaccttgaaa
      781 cggttgatgg tgtcttcgag gaagctgaag ccgcgctcga tgcgctccaa gctgatttgt
      841 ccgacggtta gatccgacat ttgtagctcg ccgtagccgc ggtagcgcgc cgccgcgttg
      901 ccgaggagac cccactcggc aaggccacac atccggatgt atagaccaaa gcgtttgcaa
      961 agctgaaggt caaatgtgct gacgccaaac tggatgcgca cggtatcaag ccgcagtttt
     1021 tcgcgcagcg gatcggccac ttggttggta aggagctggc tcgtcaccag cttcgctgcc
     1081 ggatcaccgt agtcgatggc ccgcgcgccg gtcggcgatc ctgctgtcgg atttggcttg
     1141 ccaccgcgca ccgcgtccgc cgcatcgcca agcagaagga agctggcgca gtccgccgcc
     1201 gacagtccat cggacgccag gcattcaaac ttcagctcgc ggacacgccc acgcagcgcc
     1261 acctcgacgt tgcgctgctg atcgttttgg tcaaaatcgc gcgtgccacc gagcacttcg
     1321 acatacggcg tatcgggcca gcgcgcggtc ttggaaaaca cgatcttgct gtcgcctttg
     1381 acgtggaatt cgccgcgcag accggggacg taaaagacgc cgctttcgac gcggatgacg
     1441 ccgttcatcg caatgtcgct gagcggtccc gtcacatcga acgcctgcgt cgcgagccgc
     1501 agatcagcaa tgttgttaaa cacgcggaac gtgccgcgcg tgcgcacttg cagaccgagc
     1561 cgaatctgcg acaaaaagtc gtcgccatcc caaaacggct tgtcttcttc gacgatgcgt
     1621 cgcgccgaca aaaagcgatc ggcaggatcg tagtcttgca tgtagcgacc tgcgaccact
     1681 tcgatgtcac cgcccacgct gatgccgtca cgactgccag tcagcgtcag gcctttggcg
     1741 gacgacatct cgacgttgta gacgcccgac gagctgtgac ggacattttc catcttgatt
     1801 gccaggtcga cccgctcgag cgcgcggtgc catggggtct tgatgaagct gccgatgccg
     1861 tcgtgttcga cccgcccgga cagctcgatt ttgccgtctc cgtcgacgag tcctgatagc
     1921 gcgacacccg atggactgcc ttcgccgcgc ccgatgatga tgcggccatg atcgccgcca
     1981 ccgacgcgca gaaaacggat ggtgccgcgt gaaaacgaca gctcgtggaa gcgccgcagg
     2041 ttcaaaaaca cttcgtgcgc agtcagggtg ccctcgacga tgggctcctc gaacgtgcca
     2101 aaaactttga gcagatcggt accgaaacga ccacgcgctt cggcgagatt gcgggcaaac
     2161 tgccactgaa gcagccgggc cgagatgtcg ccgcgcaact cgaacgcgat tttgccaagc
     2221 tgaagcggcg tatagcgatc gatttcgacc ccgccgcgta ccaacgtgac cgcgctgtcg
     2281 acgacaatct tgacgttttc gagatcggcg cgctggggac gcaaaagcag cttgccgcct
     2341 tgcacttcga ttggcgtgtc aagctcgtgc ggctcgacga gcgcgcggct gagttccagc
     2401 gtcccctggg gcgacaagtt cgcgagcggc ccggtgatcc gcacgtcgac gattgcatcg
     2461 ccttgtgtcc gccgaaacac cgatcgcaaa agatgttcga gcagctcgag ccgaacctgg
     2521 ccacgaaccc gcaaatccga cgccaccgga cttatcaggc cggcaaaacg caccgccccc
     2581 gcctcggtcg atgactcgcc gacgcgacgc tgactgacaa aacgggcttg gcccagctcg
     2641 atgtcgcggc catcgaagca agaaaagacc tcgccgtcgt taaatgcaag gtagcgctga
     2701 tcttcgcctt cgtcggtgac tgtctttacc gcgagctgcg cccgactgag ccgcagcgtc
     2761 gcgccaaagc gcagaagttc ttcttcgacc ggcggacgcg acgccagcat gggacagctg
     2821 atgccgcgcg cgaacaccgg atcgggcgca aacaagggcc gcggatcttc gccaaaacgc
     2881 agccgcagct cgccgcttcc cgtcaccgat acgtcgccaa gctgggccaa gtcaggcagc
     2941 agcttctggg ccagcagatc acacgcgagc accgctgccg tcggacgcgg ccccgtggcc
     3001 gaatcggctg ttttcacaaa atagggggcg agcggacagc caaaacgaat cgtcagatca
     3061 ccgcgcggca gcccgccaag gagcgacagc acaccgtcga catcgaacga atcaaacagc
     3121 cgacccgtga tcttggtgcc gctgccgctc ggtgaaaact gcagctcggc ttgcccaagc
     3181 gtgagcgctc gcaccaccat gccgagcaaa ccaaaaattg gctcgcgctc ggccaaaacg
     3241 attccaccct cgatgacggg accgcttgcc gtcggtagct tgccctgcac cgtgaagccc
     3301 gacaccgaga gcttgccatc gaagaccggc aaaagcggct gcagcgagcc catcattttg
     3361 acattgcccg acaaaatgcc gtcgaccacc agcggcagcc cttgtaccac cggcatctgc
     3421 ccgatcggca gcttgtcaaa tgtcaccgcc agttccagag gccgtccgcg ttcatgtttg
     3481 agcagcgccg tcgacgacag cttccccgtc gatagcttgg ccgacagcga cttgagccag
     3541 gcttgggttt gcgaaagctg cgccagcacc gacacatcgt caaactgtcc gccgagcact
     3601 ttcaggctgg gcacctggag gtccacttca ccgacgggtc gctgcatcgt gccagtgacg
     3661 tcgatcttgc cggtcaagag cccgctgaca tcttgaccgg gaagcagaag tccgacgctg
     3721 acctttttga cttcggcctt gaccgtcagc tgtgggtcgt cgggacgctg tgacacatct
     3781 ttggcaaaaa gctggatcga cgcatcgccg ctgacactgc cgtctttgcc gtgcgcatcg
     3841 agagacgcca agttgagctt gccgtcgtgc aaggcaaact ccgtcgtcag tgcatcaatt
     3901 ttgcgaccaa acaagtcgag gtctttgacg gcaaactccc cgcgcgatgc ctcgggacgc
     3961 aagatccgcc cgccgagacg aaagcgcgtc tgtgcctctt gcgcagcccc ggtcacgcca
     4021 aagtattgca gcacccgtcc gagcggcgcc cctcgacccg ccatttccat caccgggatg
     4081 tcgagcttgc catcgcgcgg cgagaccgca ccgtgatcgg atcgcagcga cagcgtgtca
     4141 ccgctcagcg acaggccgcg gaacagcaca ctatcgggcg tatagacaaa cgacccgagc
     4201 agcttcatgt ccttgggcag cggatcgcga cgggccagac gctcgacttg ggcatcgacg
     4261 cgatcaatca ccacgcgctc gggatgcgcg tttttggcca gcgatcccga cagcttcatc
     4321 gtccctttca gccgtcctgc gacgatgggc tgaaggattt tgggcatgat cccgacctgc
     4381 gccggatcgg ttccaatcgc cgtgatgcgc gcataccagg gaatgtcgcc ggaaaagttg
     4441 aagtggagcg gctcggcccg gatttgtccc ccgagtgcac tggcaatcac ccgcggcatg
     4501 tgaagatcgc tgcctttgag cgtgaagctg gatcgggcct cgctcacggc gatcgaggcg
     4561 atctgcgcgt ccacatcggc gacctgtccg tcgatgacaa tcgatcggcc atcgccctcg
     4621 agcggaaagt gctggacgtg aaagtcctgg cttaaacacg gcgacagtcg gccacttatc
     4681 gtcgtcggca gcgtcgaaga cagcgcgcca aagaaacgca cccggcctcg cggctgcccc
     4741 gacagaagac cgccaggaac cagcttggcc aggccgtttc cgctcgtgaa cgacatgtcg
     4801 agcgaaacgc ccgtgtcaca gtaggtatcg agcagcttgc cgtcgacttc aacatcggcg
     4861 tccgccgcct gaaccttgcc acgaaacacc aagtcctgtt tgcgactggc gcgggcacca
     4921 aagcgtcgca gctccaggtt ttttagcgga aacacaaact cgccggcacc ttcgcgcgtg
     4981 cccagaacca gctcgccacc gtcggcagtc atcggcgcca cttcgtagac gaagctctgc
     5041 aatcctggct tggtttccgc cgcatccgac gaatagcgca gcgtcaccgc ccccttgagc
     5101 ttgtcgacct gaccatgcca gccggggaac gacatttgat acgtcacgtt ctcaaatgtc
     5161 aggccctcac cgtcgatggt gatgcgcatg tggcccggcc ccggtggcgc accctcgggt
     5221 ttaggctttt tgccagacat tgtggcgacg atgttgactt cgttgccgta aagcggcccc
     5281 gtgacgttcg gtggatagag ccgacggacc tggatcgggc gaatgtgggc ccaggctgag
     5341 tgcaggtgtc ccgacgaaaa gtgcagctcg acgaaagaac cgcgattgag cggcgagctt
     5401 accgccgtgc gaatcaagcc acgaatcagc tcgcccaggt agatgtctgc ctcgatacgg
     5461 ctcgccttca tcaccagctc gccgttcgga tcgcgcattt caaagtcgcc accctcgacg
     5521 tgcgtggtgg tgttgagcaa gatcgacgcg aggctggaaa aatacgaata gcgcgcgtag
     5581 ccaagccgaa actcgccgcg aatctgcgtc ccgacgatgt ttgtgacgag cttgccgagt
     5641 cgacgatcgt tgaggccgag gaaaacccca agccacacga acgcgatcag ccccagaact
     5701 tgcagcactc gacgtaccaa ggtctggcga agcgggatga tgctcgggct tgtggaccgc
     5761 tcgctcacga aaaaaaagta acacgtccgc cgtctgggca gcaccccttc tgcgccgggc
     5821 agcgatatac ttgcgtagct atggcccgcg tcctaaaagg cggtctccgc gacggaaaga
     5881 aagcccacat cgatccgtat ccgttcgacc cccgcgccga ggctgaaaag atcctgagcg
     5941 aagccaaaca cgaagcggag cggctgcgca accaggcgat taccgaaggg cgtgagcgcg
     6001 gcctcgcggc agtgaccgag ctgctcaccg cggccagggt tcagacgctg cgcgctcaca
     6061 aagatgtcga agaagacctg cgggtgctcg cggtgcgcat cgcggagcgg attttgggtc
     6121 gagagcttgg ccaaaatccc gatgccgtcg tcgacattgt cagcgaagcg ctgtcgcaag
     6181 ccggggcgcc gcgcgaagtg gtggtgcgct gtcatcccga cgatctcaaa gcgctggagc
     6241 ggggccgacc gcgacttatc gagcgctgta gccgcaccca agcggtgctg tttcgcgccg
     6301 acgccacgat cgcgcgcggt ggctgcatcg tcgaaaccga gctggggacg gtggatgcgc
     6361 gcttgccgac gcagctcgaa gcgattgagc gggccctgcg aggccaaggt ccatgagcga
     6421 gccgcaaggc cgaagcgatc gcaaagaccg cgccgatcgc cccgatccgc tgcgcctgtc
     6481 tgccgcgctg cgcacggtgc aattgacggt tccccagcgg atttccgggc gcgtcagcga
     6541 gctgaccgga ctggtgctgc gagcgacagc accgggtgcg cgcatcggcg aactggttgc
     6601 catcgatcgc ttcaacgaac ccatcgacga caaccggcac agcgcaagct tgccgcactg
     6661 gcgacgcggt ggcacaggtg aaacgccgct ctatgccgaa gtggtcggtt ttcgcggtga
     6721 agaggtcgta cttttgccgc tcggttccgt cgctggtgtt ggtcccagtt cgattgttcg
     6781 cccgaccgga atgccgctga ccatcttgac cggaacgccg ctgctcgggc gagtcgtcga
     6841 cggctatggt gaagcgatcg acgacggtcc gccgctgtcg agcatgctgt cggaactgct
     6901 cccaaccccc gtcgagcgtg aagcgccaga gccgctgcgt cgtcgccgca tcaccgagcc
     6961 gctgtcgttt ggtctgcgcg ccattgatgg ctgtctcacc gtcggacaag gccagcgttt
     7021 gggtctgttt gcagggcctg gtcttggcaa atcgacgctg cttggccaga ttgcccgcaa
     7081 tgccagctcg gatgtgacgg tgattggcct gatcggcgag cgtggtcgcg aagtgcgtga
     7141 ctttatcgat cgcgagctgg atgacgaaac ccgtcgccga accgtggtcg tctgtgcgac
     7201 atcggatcag ccgccgcttt tgcgtctgaa agcagcgctt tccgcgacgg cgattgccga
     7261 acacttccgc gatcgcggca aaaaggtcct gctgctcgtc gattcgctga cgcgggtggc
     7321 acgggcgcag cgcgaagtcg gtctggccgc cggtgaactg cccgcccggc aaggctatcc
     7381 accgtcggtg tttgcgcttc tgccgcggct tgtcgaacgc accgggcaag gcgaatttgg
     7441 ttcgatcacc gcggtctatg ccgtgctcgt cactggatcg ggggacgatg tcgacgacat
     7501 cattgccgaa gaagtgcgcg ccacgctcga cggccacatc gagctgtccc gttcgctggc
     7561 cgagcgaggt cactacccgg cgatcgatgt gctgcgctct ttgtcacggg tgatggacga
     7621 aatctgcgac gcgacccagc gcggggctgc cgccaagctg cgcgagcttt tgtcgctgtc
     7681 ggaacggcaa cgggacttgg tgctgctcgg ggcgtaccag cgcggcagcg atccagccac
     7741 cgacgaagcg cttcagaaat tgccagcgat tgaggaattt ttgcgccagg gtcgctacga
     7801 gcgcagcgac ttggctgaga cgagaaagcg gctgaccagc ctagttcggt agaccaccgg
     7861 ggaacggcat cccgccagga ccgggaggcg gcgcagccgg cggcggattt ttgtctttgg
     7921 ccagatccgc aggcttcttg agcagccggt caagctgcat cttgcgcacc cacatcgcgt
     7981 cgctgccggc cttttgcacc gccaggtctt cgccgcgcgc gtcgccgact ttcagcacga
     8041 tcggtgcgcc ggtctttggc ttcaccgtga tcgtcgcctt gcccggcaca agcgacttga
     8101 gctccggcgc atccggggcg acaaagcccg ttcccgcaag accatcgaag ccttcgacga
     8161 tctgcttgat cttcgcctcg tcagcgtccg gaagcttgtc ggctttccag gtcttgtcga
     8221 cgagcttgag gctgaccaca tcggttcctt gctgaatcgt caccgcatcg atctggtcgg
     8281 ctttcatcga cagcaggctc ttttcacgaa cgtcttgcgg aatgtgcgcg atcgactcga
     8341 tggcgtactt cttgacggtg aaaatctgcg cgctgtcgac ggtctggacg aacgtgtctt
     8401 cgcccttcac ggcgccaatc cgaacgcccg cgctcttgcc ctctttgtat ccgaccgaaa
     8461 ccacgatctg tcccgcagca ccggctgcca gaccgacatc ttcgggcttg gctgcgtcgt
     8521 ggaaatctga cgcacgcagc gtcgacaccg actgaaccat ccgattgagc aagctgttgt
     8581 cgaccgcaag actcggcgtc agcgtcgcat cgccttctgt gatcttccac ttcgcttcgt
     8641 agatgttctg actgcctggc ttgccatcgg cgcccgccgg aaccggctct cgcacaagcg
     8701 tcactttggt gccggcgcct tggatcgtga tcttttccga gtcgtcggct ttcaggtcgt
     8761 agatgctgtg ttcgcgccag gtcttgcttt cgcgcttgta cgtcgatgaa tacaggtcga
     8821 tggcctgcca cacgtcgtct ttgccggcga ctcgaaccat ggtggcactg ccgctcgctt
     8881 tgccaatgaa gacatccgcg atcaccgcgc cgcccgaatc tttggcgacg acgtgaacag
     8941 ctttgtcgtc ggtcacttca aactcggcgt gacgggtctt ctgctgcgtg accagatcgc
     9001 cccagcggac t
//
LOCUS       DAORGN010000205         8952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3437_length_8952_cov_5.388221, whole genome shotgun sequence.
ACCESSION   DAORGN010000205 DAORGN010000000
VERSION     DAORGN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_8952_cov_5.388221"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKL17_17845"
     CDS             <1..702
                     /locus_tag="PKL17_17845"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK46650.1"
                     /translation="RGTVLLDEIGDMEPVLQGKLLRVLEERTFERVGGVRPIQLEARV
                     IAASHRDLSQLVAEKKFRLDLYHRLSVFPIQIPALRDRGHDIVLLAEHFLGEFGDRMG
                     KKLQLHRETSELLLRYDFPGNVRELRNLMERAAILAPSVASGQSLVTPELLPPRVAEI
                     TVQRQLVTPQALGPRSDSGLTVTFEPGRDTLENLERRLLEEALRMAGGKKVKAAEILG
                     ISRFALLRRVEKYGL"
     gene            complement(703..1257)
                     /locus_tag="PKL17_17850"
     CDS             complement(703..1257)
                     /locus_tag="PKL17_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNK46651.1"
                     /translation="MRVVLCLVGLLAFFGPSPARAIHENPALIESELLHRRLKLDGLL
                     AWNAARQRFDGWSENPSAKITVLHLWSPRCGPCVAELPFLQRLFAAWRSEPAVRFLLV
                     ADFSEDSDGSDVVSFVDSHRAQFPAGPILRLRDSSLREVLGTSVQPLTLLLDEKQVVR
                     QVFVGPLGGRSLGSALQRLLATLR"
     gene            complement(1261..2421)
                     /locus_tag="PKL17_17855"
     CDS             complement(1261..2421)
                     /locus_tag="PKL17_17855"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46652.1"
                     /translation="MDADSKLLAYLTAASELLRDGKLADAGNAIAEALRIAPQDVRAL
                     NLLGLLRFQGGQVDEAHAVYARLVSEHPGDPALRLHLGLCELRRGQPAEAVVHLGLVV
                     RSEPDNLRALSYYGLSLLRAGRLVEARPILLRAGQTELVAEVDQKLSTQPPSPETLPD
                     SGPPKKPPRIVEPLVEEPQLPTAPPLADVLSQVTIGEAPALFSLTSDGLLLIRVVGHV
                     LLRGEGVVASVGTLDFVPLGDESSDGSGQVPELLFRAEGRGELLLSPQGGRFTPLSLG
                     DGEELCLAPQALLACSSSLLRDPRVLPRSEGELPCLLLKGPGELVLRSPCELHALPLR
                     PGQSAYLDESSLLGFTAGLLVMRDALSDDENDGHLVCSGHGHVLLSVKHHGP"
     gene            complement(2436..2933)
                     /gene="folK"
                     /locus_tag="PKL17_17860"
     CDS             complement(2436..2933)
                     /gene="folK"
                     /locus_tag="PKL17_17860"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNK46653.1"
                     /translation="MDAVIGLGSNLGDRLRFLRSAATELMHVGRICSRSAVYETDPVG
                     GPPQPRYLNAALWLVTELLPDALLAVLQRIEHHAGRHRDGERNQPRTLDLDMLLLGSR
                     GELVMVTHDLVVPHPRLHQRAFALRPVLDLEPTLVHPALGVPLSDLLACLPSEDIQTQ
                     PIGWL"
     gene            complement(2921..3667)
                     /gene="tsaB"
                     /locus_tag="PKL17_17865"
     CDS             complement(2921..3667)
                     /gene="tsaB"
                     /locus_tag="PKL17_17865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNK46654.1"
                     /translation="MRILAIDTATQLCGVALLDTDSGRLARRQQVVTTHSESLLSLVT
                     ACLAELDLPVRDLSVVALGAGPGSFTGLRIGCATAKGLCYALSIPLCMVSSLLALAQP
                     LCGSRSVLATQDAFRGQVYARLMPSPHASPGLRKLMEQQPALLTDAAWNPDELATLMM
                     PFSGELLLGGGGALRYPVLSALPNVAIDPAVAPDPVVVARIGLSLVAEGKTAHLASAL
                     PTYVTVCAAEEHAAPIGEFSAGAGSVAWTR"
     gene            complement(3701..4444)
                     /locus_tag="PKL17_17870"
     CDS             complement(3701..4444)
                     /locus_tag="PKL17_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK46655.1"
                     /translation="MSEVVLETRKLVRTLGDKVKQVILDEITIQVRRGEFVALTGHSG
                     SGKSTLLYLLGVLDKPTLGQVLVDGQDTSTLDDDERAALRNDKLGFVFQFHFLLPEFS
                     VLENVMVPMLRRGFPSARAQKQAVTTLEYLGLGELSKRRPNELSGGQQQRVSIARALA
                     GKPTILLADEPTGNLDSKNGEIVIQLFEKLNREQGTTLVMVTHDPLFAERASRRVSLK
                     DGRVIADSGSPVLPTDGLHSVPKASASAS"
     gene            complement(4441..5697)
                     /locus_tag="PKL17_17875"
     CDS             complement(4441..5697)
                     /locus_tag="PKL17_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK46656.1"
                     /translation="MRLIFFIALRQLWDRKLLNGIALGGVALGVLVLISMNAVMQGFQ
                     QKFKGEILKVSPHVTMYDKELGSQQGILETLLKPAEVAKLVHHQQPSDRVTRIKRPRD
                     LRRELQEMPQVEAACTSLTGQAIVSAGTVDLGVDMRGVVPLEQEACTPLGRYVQSGSW
                     RTLGITPDGIAMGSGVADKLGVKTGDQVRIVAPGGLPQNLKVVAIYEAGVPPIDKTRV
                     YVNLTTAQTVLRRADVVGRIELRLWDPFESQKLAMRLERISGYDVESWQEANANFLAL
                     FDMQNMIINMVIAAILVVGGFGILAVQIMIVLQKTRDIAILRSVGLRRRDILLIFLIQ
                     GIIVAMLGALIGDVAGWQLVEFLGTLKVKTEALVKTSTFLVYKDPMFYLYGTVFALLT
                     GISASLLPAVRASRVEPVDVLRGQVG"
     gene            complement(5727..6923)
                     /locus_tag="PKL17_17880"
     CDS             complement(5727..6923)
                     /locus_tag="PKL17_17880"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK46657.1"
                     /translation="MQARWILLPLLLLSAGAVGYSKFLRPTTVQAATVVRGVAVEAVY
                     ASGTVEAVDRVEVKARLSGPVGQLLVREGDKVSQDQLLAQVDAPTLNFDVTRGRADFE
                     AALERSSTAPQVAALAGQESALRAQLAQAKADLARADTLNKSGAGVALEVEKARTQVA
                     ALEAQISAIRAQEQDVKIALKADRLRSKAGVDSLRARARDAEVRSPMAGVVLKRRIEK
                     GEVVATNQNLFRIGDLTRLWIESRVDESDIGRVRDNMAAAVRLYAFEGKVFPAHVSRI
                     LPDADRDRKSFEVDVELDEAVPGLLPGMTAEINIIVRKHENVLLLSTDAVREKGGLHV
                     WLIGEDGRVHRQPVRIGIRDLVQVEVTEGLGEGQVVALEDEDQLREGKRVSPQLKAPA
                     RTAAAP"
     gene            complement(7043..8872)
                     /locus_tag="PKL17_17885"
     CDS             complement(7043..8872)
                     /locus_tag="PKL17_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46658.1"
                     /translation="MSFRRCEIPSSTTPPPSEASQTPFRGSSLGRTSRMARSLTLLGC
                     LGLATQMTCGQGRPDGEQNLDENNPDQVRLVSLTLKPNNDILLVDLNAEATKAFTVRG
                     LYSDGSSADFTRKVRFSLDNNAVGKFSGPTFKSAVLEKNKVDFSKVYAKVTDNGRELA
                     TVANLTVVWLRTSGNSQDFFFNLPYMISPQTKPLTFSTQVQSLDVFFGVDTTGSMGGE
                     INQLVRSLQSTIIPGVKAAAAKDAWFGVGSMDDWPYGGYGSPGCNGVHDQPLVLLQPM
                     TDDVMKAQNGVQQLLRGSSPAGCGADTPEGQMEALYQIATGDGQTNAGANVPANHKGI
                     GGAEFRDGALPVVVTISDASFHDVGDMPPRTCYGSPVSYSGEVAKYGHTRRQTADALN
                     KICAKAVGISAMLSSDENCIATKDMIYFAKETGAVVPPEAWDVPMRPNGCAAGKCCTG
                     LRGAGEEPDTNGLCPLVFKVQSDGTGVGDQVTAGIAQLAQFASFDVVTDVVGQKQGEK
                     GEIIPDPKTTADFIKSITPLDALPPPPPPFIPAPVMTEKGFTKVVPGSFVRFNVTAHN
                     EIVKETFRPQVFHATIRVRAGGCANLDSRDVIILVPPVAPTPG"
BASE COUNT         1818 a         3000 c         2528 g         1606 t
ORIGIN      
        1 cgcggaaccg tgctgctcga cgagatcggc gacatggaac cggtgctgca aggcaagctg
       61 ctgcgcgtgc tcgaagaacg cacgttcgag cgcgtcggtg gcgttcgtcc gattcagctc
      121 gaagcccgcg tgattgcggc gtcgcatcgc gatctgtcgc agcttgtcgc ggaaaagaaa
      181 tttcggctcg acttgtatca ccggctgtcg gtgtttccga tccagattcc agcgctgcgc
      241 gaccgtggtc acgacattgt gcttttggcg gaacactttc ttggcgagtt tggcgatcgg
      301 atggggaaaa agctccagct ccatcgtgag acgagcgagc tgcttttgcg ctacgacttt
      361 cccggcaacg tccgcgagct gcgcaacctg atggaacgcg cggcgatcct cgcgccgtcg
      421 gtcgcaagtg gtcaaagcct ggtcacgcct gagcttctgc cgccgcgagt cgccgaaatt
      481 accgtgcaac ggcaactggt gacgccgcaa gcgctgggtc cgcgcagcga cagcggtctg
      541 acggtgacat ttgaaccggg ccgcgacacc ctggaaaacc tcgagcgccg tctgctcgaa
      601 gaagcgctgc gcatggcggg cggaaaaaaa gtcaaagcgg cagagatcct cgggatttcg
      661 cgctttgccc tgctccgccg cgtcgaaaaa tacggtctgt gatcagcgca gcgtcgccaa
      721 cagtcgctgc agcgcgctgc ccagactgcg accaccaagc ggcccgacaa agacctggcg
      781 caccacctgt ttttcgtcga gaagcagcgt aagtggctgc acgctggttc ccaacacttc
      841 gcgcagactg gaatcccgca gccgcaaaat cggtcccgcc ggaaactgcg cacggtgcga
      901 atcgacaaac gaaaccacgt cacttccgtc gctgtcttcg gaaaaatcgg cgaccagcag
      961 aaagcgcacc gccggttccg atcgccacgc cgcaaacagc cgctgcaaaa acggcagctc
     1021 ggcgacgcac gggccacagc gcggcgacca cagatgcagc accgtaattt tcgcagacgg
     1081 attttcagac caaccgtcaa agcgctgccg ggccgcattc caagcaagca gtccgtcgag
     1141 cttcagccga cgatggagca gctcgctttc gatgagcgcc ggattttcgt ggatcgcacg
     1201 cgctggactt gggccaaaaa acgccagcag tcccacaaga cagagcacaa cccgcatcgg
     1261 ctagggcccg tggtgtttca ccgaaagcag cacatgccca tggcccgagc agaccagatg
     1321 cccgtcgttt tcatcgtcac ttagggcatc gcgcatcacc aaaaggcccg ccgtaaaacc
     1381 aagcaggctg ctttcgtcga gatacgcgct ctggcctggc cgcaaaggca gcgcatgcag
     1441 ctcgcacggc gaccgcagca ccagctcgcc cggacctttg agcaaaagac agggcagctc
     1501 gccctcgctg cgcggcagca cccgcggatc acgcagcaaa gacgacgaac acgcaagcag
     1561 cgcctgtggg gccagacaca gctcttcccc gtcgcccagc gaaagcggcg taaagcgccc
     1621 accttgcggt gacagaagca gctcaccgcg gccctcggca cggaacaaca gctcgggaac
     1681 ctggccactg ccgtcgctgc tttcgtcacc taacggcaca aaatcgagcg ttccgacgct
     1741 ggcaaccacg ccctcgccac gcagaaggac atgccccaca acccggatca ggagcagtcc
     1801 atcgctggtc agggaaaaca gggccggcgc ttcgccaatt gtcacctgcg aaagcacatc
     1861 ggcaagcggc ggcgccgtcg gaagctgtgg ttcttcgacc agcggttcga caatccgcgg
     1921 cggttttttg ggcggaccgc tatccggaag cgtttccgga ctgggcggct gcgtgctgag
     1981 cttctgatcg acttcggcaa ccagctcggt ctgcccggcg cgcagcagaa tcggcctcgc
     2041 ttcgacgaga cgcccggcgc gcaaaagcga cagcccgtag tagctcagtg cccgcagatt
     2101 gtctggttca ctgcgcacca caagccccag atgaaccacc gcttcggcag gttggccgcg
     2161 acgcagctcg cacagaccca ggtggagccg cagcgccgga tcgcccggat gttcactgac
     2221 aagccgcgca tagaccgcgt gcgcttcgtc gacctgccca ccttgaaaac gcaaaagacc
     2281 aagcaggttc agcgcccgca catcctgtgg tgcaatccgc agcgcttcgg caattgcgtt
     2341 gcccgcatcc gccaattttc cgtcgcggag cagctccgaa gcggcggtca aataggcaag
     2401 cagttttgaa tcggcatcca tggccagtct cgatactaca accagccgat gggctgtgtc
     2461 tggatatctt cactcggcaa acaggccaac aggtccgata aaggaacacc cagcgccgga
     2521 tgtaccagcg tcggttccag gtcgagcacg ggacgcagcg caaacgcccg ctgatgcaga
     2581 cgaggatgcg gcacaaccaa gtcgtgcgtc accatcacca gctcgccgcg cgacccgagc
     2641 agaagcatgt ccaaatcgag cgtccgcggt tgattgcgtt cgccgtcgcg gtgtctgccg
     2701 gcatgatgtt caatccgctg tagcactgcc aaaagtgcgt cgggaagcag ctccgtcacc
     2761 agccacagcg cggcgtttag atagcgcggc tgcggtggtc caccgacagg atcggtttca
     2821 tagaccgcgg accgcgagca aatccgacca acgtgcataa gctccgttgc tgcggaccgc
     2881 aaaaaacgca agcggtcacc gagatttgat ccaaggccaa tcaccgcgtc catgccacac
     2941 tccccgcccc agccgaaaat tcaccgatgg gcgcagcatg ttcttcggca gcacaaaccg
     3001 ttacatacgt cggcagcgcg ctggccaaat gggcggtttt tccctcggcc acaagcgaaa
     3061 gaccgatgcg cgcaaccacg accggatcgg gtgccacagc cggatcgatc gccacattgg
     3121 gaagcgccga tagcaccgga taccgcagcg ccccgccgcc gcccagcaga agctcgcctg
     3181 aaaacggcat catcagcgtc gccagctcgt cgggattcca agccgcatcg gtcagcagcg
     3241 ccggctgttg ttccatcagc ttccgcagtc ccgggctcgc atgcggcgac ggcataagcc
     3301 gcgcatagac ctggccgcga aacgcatctt gcgttgccag caccgatcgc gaaccgcaca
     3361 gtggctgcgc cagcgccagc agcgacgaca ccatgcaaag cgggatcgac agcgcgtaac
     3421 aaagaccttt cgccgtcgca cagccaatgc gcagacccgt aaacgaaccc ggtcccgccc
     3481 cgagcgccac caccgaaaga tcgcgcacag gcagatccag ttcggccaga cacgcagtga
     3541 caagcgacaa gagcgattcc gagtgcgtcg tcaccacctg ctgtcttcgc gccaaccggc
     3601 cactgtctgt atcaagcaac gcaactccgc acagctgcgt cgctgtatcg atcgccaaga
     3661 tcctcacgtc cggccttcta ccacgagcga cgccggcttt ctagctcgct gacgccgacg
     3721 ctttgggcac actgtgcagt ccatccgtcg gcaaaaccgg gcttccagaa tcggcaatca
     3781 cccgcccgtc tttgagactc acccgtcgcg aggcgcgctc ggcaaacagc ggatcgtgcg
     3841 tcaccatcac cagcgtcgtg ccttgctcac ggttgagctt ttcaaaaagc tggatcacga
     3901 tttcgccgtt tttgctgtcg aggtttcccg tcggttcatc cgccaaaagg atcgtcggct
     3961 tgccggccag cgctcgcgca atcgaaaccc gctgctgctg accgccggac agctcgttcg
     4021 gtcgccgctt cgacagctcc ccaagaccca aatattccag cgtcgtcacc gcttgttttt
     4081 gcgcccgcgc acttgggaaa ccgcgccgca gcatcggcac catcacgttt tccagcacgc
     4141 tgaattccgg caaaagaaag tgaaactgaa agacaaaacc aagcttgtcg ttgcgcagcg
     4201 cggcccgctc gtcatcgtcg agtgtgctgg tgtcctggcc gtcgaccagc acctggccca
     4261 acgtcggctt gtcgagcacc ccgagcaggt aaagcagcgt gcttttgccc gatccactgt
     4321 gtccagtcag cgccacaaac tcgccccgcc ggacttggat cgtgatctca tcgaggatga
     4381 cctgctttac cttgtcgccg agcgtgcgca caagcttgcg cgtttccaag accacttcgc
     4441 tcatccgacc tgacctcgca gcacgtcgac gggctcgacg cgactggcac ggaccgcggg
     4501 cagaaggctg gccgaaatgc cggtcaaaag cgcgaacacc gtcccataca gatagaacat
     4561 cgggtctttg tagacgagaa acgtgctcgt cttgaccagc gcttcggtct tgaccttcag
     4621 cgtgccgagg aactcgacga gctgccagcc cgccacatcg ccaatcagcg ccccgagcat
     4681 ggcgacgatg atgccctgga tcaaaaaaat cagcaggatg tcgcgccgtc gcagaccgac
     4741 cgaccgcaaa atcgcgatgt cgcgcgtctt ttgcaaaacg atcatgatct gcaccgccaa
     4801 aatgccgaag ccaccgacga caaggatcgc ggcaatgacc atgttgatga tcatgttttg
     4861 catgtcgaac agcgccaaaa agttcgcgtt ggcttcttgc cagctctcga cgtcgtagcc
     4921 gctgattcgt tccagccgca tcgccagttt ttggctttcg aacgggtccc acagccgcag
     4981 ctcgatccgc ccgacgacat cggcccgccg aagcaccgtc tgcgccgtcg tcaagttcac
     5041 gtagacccgc gttttgtcga tcggcggcac acccgcttcg taaatcgcca ccactttcag
     5101 gttctgcggc agcccgccgg gcgccacgat ccgcacctga tcgccggttt tgaccccgag
     5161 cttgtccgcc acccccgatc ccatcgcgat cccatcgggc gtaatcccca gcgtgcgcca
     5221 cgaacccgac tgcacatagc gccccagcgg tgtacacgct tcctgttcga gcggcaccac
     5281 tccgcgcatg tcgacgccaa gatccaccgt ccccgccgac acaatcgctt gcccggtcag
     5341 cgacgtacac gccgcttcaa cctggggcat ttcttgcaac tcccgtcgca gatcgcgcgg
     5401 tcgtttgatc cgcgtcacac ggtccgacgg ctgctggtga tgcaccagct tggccacttc
     5461 ggcgggcttt agcagcgttt ccaaaatccc ctgctgcgac cccagctctt tgtcgtacat
     5521 cgtgacgtgc ggcgacactt tgaggatttc gcctttgaac ttctgctgaa acccctgcat
     5581 gaccgcgttc attgaaatca gcaccagcac accgagcgcc acaccaccca gcgcaattcc
     5641 gtttagcagc ttgcgatccc agagctgacg cagcgcgata aaaaaaatga gccgcatggc
     5701 tggccacact cctttggcga cggaacttac ggagccgcag cggtccgcgc cggcgctttg
     5761 agctgcggac tgacccgctt tccttcccgg agctgatcct cgtcttcgag cgcgaccacc
     5821 tgcccttcgc ccaggccttc ggtgacttcg acttgcacca gatcacgaat cccgatgcgc
     5881 acgggctgcc gatggacgcg cccatcttcg ccaatcaacc agacatgcag accgcctttt
     5941 tcgcgcaccg cgtccgtcga caaaagcagt acgttttcgt gcttgcggac gatgatgttg
     6001 atctcggcgg tcatgcccgg caaaagccca ggcaccgctt cgtcgagctc gacatccact
     6061 tcgaacgact tccggtcgcg atccgcgtcg ggaagaatgc gcgacacatg cgccggaaac
     6121 accttcccct cgaacgcata cagccggacc gccgctgcca tgttgtcgcg cactcgcccg
     6181 atgtcgcttt catcgacccg cgattcaatc cacaaccgcg tcagatcacc gatgcggaac
     6241 aggttttggt tggtcgcaac gacttcgcct ttttcgatcc gccgcttgag caccactccc
     6301 gccatcggcg accgcacctc ggcatcgcga gccctggccc gcaggctgtc gacgccagct
     6361 ttgctgcgca gccgatcggc tttgagcgca attttgacgt cttgctcctg cgcgcggatc
     6421 gcacttatct gcgcttccag cgccgcaacc tgggtgcggg ccttttcgac ctcgagggcc
     6481 acaccagccc ccgatttgtt gagtgtgtca gcgcgcgcca gatccgcttt ggcctgtgca
     6541 agctgggccc gcagcgccga ttcctgaccc gccagcgccg ccacttgcgg tgccgtactc
     6601 gatcgctcca gcgcggcttc gaagtccgcc cgaccgcggg tcacatcgaa gttcagcgtc
     6661 ggtgcatcca cctgcgcgag aagctgatct tggctgactt tgtccccttc gcgaaccaag
     6721 agctgaccga ccggaccaga cagccgcgct ttgacttcca cgcgatcgac agcctcgaca
     6781 gtgcctgacg catagaccgc ttccaccgct accccacgaa ccaccgtcgc tgcctgcacg
     6841 gtcgtcggtc gcaaaaactt gctgtagccc acagcccctg cggaaagaag gagcagcggc
     6901 aacaaaatcc agcgtgcttg catgttcgta gtttctagga cgacgccgcc gagcgcgcag
     6961 cgtcagcaaa aaagcaaaag cggcacgcca acctgacatg ccgctttctt ttggccagaa
     7021 ggggtcaaag ccccaggtcc gactagcccg gcgtcggagc gaccggcgga accaggatga
     7081 tcacgtcacg cgaatcgagg ttcgcgcagc cgcccgcacg aacacgaatc gttgcgtgga
     7141 acacctgcgg acggaacgtc tctttgacga tctcgttgtg ggcggtgacg ttgaagcgaa
     7201 cgaagctgcc cggcacgacc ttggtgaagc ctttttcggt catcaccgga gccggaatga
     7261 acggcggcgg cggcggcggc agcgcatcga gcggcgtgat gctcttgatg aagtccgcgg
     7321 tggtcttcgg atcgggaatg atctcgccct tttcgccttg cttctgaccc accacgtcgg
     7381 tcacgacatc aaatgacgca aactgcgcca gctgcgcaat gcctgcggtg acctgatcgc
     7441 caacgccggt tccgtcggat tgaaccttga agacaagtgg gcacagaccg ttggtatcgg
     7501 gttcttcacc cgcgccgcgc agacccgtgc agcacttgcc cgcagcgcag ccgtttggac
     7561 gcatcggaac gtcccacgcc tcgggcggaa ccaccgcgcc agtctctttg gcgaagtaga
     7621 tcatgtcttt ggtcgcgatg cagttttcgt cgctcgatag catcgccgaa atgccgacgg
     7681 ccttggcgca aattttgttc agcgcatccg ccgtttgccg acgggtgtga ccatacttgg
     7741 cgacttctcc cgaatagctg accggcgatc cgtaacaagt ccgcggcggc atgtcgccca
     7801 catcgtggaa cgatgcatcg ctaatcgtca cgacgacagg cagcgcacca tcgcggaatt
     7861 cggcgccgcc gatgcccttg tgattggccg gcacattggc gcccgcattc gtttggccgt
     7921 cgcccgtcgc gatctgatac agcgcttcca tctggccttc gggcgtatcg gcaccgcaac
     7981 ccgccggcga gctaccgcgc agaagctgct gcacgccgtt ttgcgctttc atcacgtcgt
     8041 cggtcatcgg ctgcaaaaga acgagcggct gatcgtgaac gccattgcag cccggcgatc
     8101 cataaccgcc gtagggccag tcgtccatcg agcccactcc gaaccaggca tctttggccg
     8161 ccgcggcttt gacgcccggg atgattgtgc tttgcagact tcgcacgagc tggttgattt
     8221 cgccgcccat actgccggtg gtgtcgacgc caaagaacac gtcgagcgac tgcacctgcg
     8281 ttgaaaaggt cagcggcttg gtctgcggcg agatcatgta cggcaggttg aaaaagaagt
     8341 cctgcgagtt accgctcgtc cgcagccaga ccaccgtgag gtttgccacc gtcgccagtt
     8401 cacgaccgtt gtcggtgact ttggcgtaga ccttcgaaaa atcgaccttg ttcttctcga
     8461 gaacagcgct tttgaacgtc gggccgctga acttgccgac ggcgttgtta tcaagcgaaa
     8521 agcgcacttt gcgggtgaaa tcggcgctgg aaccatccga atacaggcct cggacggtaa
     8581 atgccttggt cgcttcggcg ttcaggtcga cgagcaggat gtcgttgttc ggcttgagcg
     8641 tcagcgacac caagcgaacc tgatccggat tgttttcgtc gagattctgt tcaccgtcag
     8701 ggcggccctg gccgcaggtc atctgggtcg ccagacccaa acaacctaac aatgtcagcg
     8761 agcgtgccat gcgcgaagtc cgtcccaaag acgacccgcg gaagggggtt tgactggcct
     8821 cggatggagg cggggtggtc gacgacggga tctcacaacg gcgaaaagac atggcggaat
     8881 cctcccactt ccgcaaacct agtacgaacg caaccgacat ttcaacacca gaatcggtgc
     8941 cgcatttgac ag
//
LOCUS       DAORGN010000206         8904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3475_length_8904_cov_5.855351, whole genome shotgun sequence.
ACCESSION   DAORGN010000206 DAORGN010000000
VERSION     DAORGN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_8904_cov_5.855351"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKL17_17890"
     CDS             <1..386
                     /locus_tag="PKL17_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNK46659.1"
                     /translation="TYIDGKRCWVNKVIYGDASPTAINPRPDGKRQLFAAGDSNTDVT
                     FVRDAEKLKLVINRNKAELMCNAYGNFGNRYLVNPMFIDPKPQQTALYPCTTTACVNS
                     NGMATPCLDEGQGPIADQKDAVYPM"
     gene            453..1271
                     /locus_tag="PKL17_17895"
     CDS             453..1271
                     /locus_tag="PKL17_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNK46660.1"
                     /translation="MTADRQYQSVSYRPCELEHRYGPSVHILRDPLALSLLAKLCHRD
                     VVQPEVSRLIVELYRVLVHEVVAAEFPRQQSSIPTRMIQYVPEGVYDGEVIAESTPAV
                     VVALARAGLLPSQVSYDFLNQVLSPSGVRQDHLALGRTLDGAGHVTGAGLYASKIGGP
                     VDGAVMLIPDPMGATGSTVSRVLRHYEKEVVGRPAKVVAVHLIVTPEYLRHVTKNHPE
                     VVVYALRLDRGLSEPDVLETVPGTHWDRERGLTDHQYIVPGGGGLGEVLNNSFV"
     gene            1297..1869
                     /gene="hpt"
                     /locus_tag="PKL17_17900"
     CDS             1297..1869
                     /gene="hpt"
                     /locus_tag="PKL17_17900"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNK46661.1"
                     /translation="MSDQDTFVEPKLSPLFTEEQIAARIAELGAQIAADYQRRHLTLV
                     TVLKGSFVFAADLSRAIQRAQVAQGLPGSTVTCEFLGLSSYGDETVTSGVVRITSDLS
                     NPIEGSDILIVEDIVDTGLTMSYLLENMRTRHPASIRICSLLHKPSRTKKRVPIDYIG
                     FTIEDLFVVGYGLDYKQRYRNLPWIGVLSL"
     gene            1964..5869
                     /locus_tag="PKL17_17905"
     CDS             1964..5869
                     /locus_tag="PKL17_17905"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK46662.1"
                     /translation="MSNQARPKDAAVCADCGHVLEEDMPHICQGAAKKSAFDSTIGPG
                     DHPTIPGEVSSADIHDPAQELIGKIIGERYEIQRLLGQGGMGVVYQARHISLEKLLAV
                     KLLLIAQNEEYQRRFLQEAQLASQINHPNTVFLSDFGVLPDGRSYIVMELLRGPTLAS
                     VIAEGAMEPMRVCKIAAQIAHGLQAVHDQGIIHRDLKPENVFLLEQDGKKDNVKIVDF
                     GIAKATSEAPVVRKRHSQEDISQVTEQKISSQTMPGTLMGTPAYLAPEMALGQEVDFR
                     VDQYALGVILFEMLTGDLPFEATTIGQMLQHHISTKPPLLSRKISDSSPTLDALVARL
                     LSKKPVERFPSMRAVAEALEKEVELRLLARGDTALPAHIAEALSKTQAGTHIRIAGRK
                     VPLWVLLPVLMIFVVAGGAASWLLWRQRVEQVGITPEELAELRQKALDVVREYAKGDD
                     APLRALSAQALSQTRDPSQRPQLEALLGDAAPQVRVRAALALGAVGDRQATSALRAAL
                     SGPIEVKLAAAMALSQLADSDGHKVLVDALAAKEPAVQKQAALYLCELGDEAARQVLS
                     DLHNRSGLGDDEDFLALQCLVRFGDEPARVELAAKVEAGQNRKQKLEAMFRLAELGDE
                     PTRTELRQIASKRGGEQLLTANRLASPEEPQLLAVFRPILANEFGVPTGRQLAAEGVG
                     KSGELPQIKELGPLLDGKVVAVVRQTAAAAILRLASGDPGLLTTASLSWAQNELGSGS
                     ASRRLLALQVLGDLPSSQAVALLSTAMQDGSADIRHAAAKALGRRKERTAMTLLLESV
                     ADRDEKVRYASLLALLDLVGRLSKRDAAGLLQAAEGVLKEILQKGSVLEQALASSVLL
                     RLGDKKQVDRLKALRSDHDPQIRHLILDALSSDHDFVALFLSDAIDSVRLHAARILAQ
                     SGDKRAVPVLKAFFSVGGVDGLHAAGLLRRLGIRENLPPDLLRLLHSASVEERVAAVR
                     ALRDVDVTLALSLLRPAAADPAAEVRRAVAEVASSLPKGPGGYAGVPLLRQLSQDNEA
                     TVWTLARSLLARLLTPEDAPIVEPTLPPRRSAFAPEPPKPAAMETEPPTGTVTGKLSV
                     VAAEPVVFQVGKRPWQQAPATVELPVGKHMVATLATEQEVNIVASGEPYKLELSESPA
                     EKFLRIGIAAAKGGDSKKALTNLERARAICQKDRKHSHDPPCHSIVFDASYYQGRMHE
                     NAKDLHLAMEAYQKALEQGEKVKGRTDLKNQLVEITGRLRGRLGQVIVKSLVNGRCQS
                     KVYWMIPAKHQVRDGQGRSISVDLRAGDQREVGSCDGDK"
     gene            5872..7464
                     /locus_tag="PKL17_17910"
     CDS             5872..7464
                     /locus_tag="PKL17_17910"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46663.1"
                     /translation="MSCVFRRNSRLRKAVLLSLIGHLLVPSFSAGKSRSTPQIQKRSR
                     QDQEVSAQLVKDAEKAITEQNWVDAQRLLTDAYLKFPGPQILYHLGVVAAKEGRLLDA
                     HDLLRRYESDPASTPSQAMTAEIQRLLAKQRPPSGKVLVLGDDGAIVRVDGRVVGSLP
                     LVQPLLVSPGDSHTIALDFPEQQIPAQVTISAGRFAELRINRATRAVLLTVLPAFVVL
                     SEYRDVPQPSMVKLDEAIEAGVQGEKKSVLRREIALRQAPELASCLDQIDCQAKLAEK
                     TEVDGLIRLSVENTAARTGGAPSYKLTLQLLDPTVGAIAGQASRELPPERATTAMTEM
                     LSQIIGDAALRRRGLISVRSNPDGAEVYAGERMLGKTPFQRAAWVGRYDLTFRKPGFA
                     PESAQTEVTDGKTSELEVSLKPGVTLPPPPPPSRFDFVFTPHDFRPPRPFWRWLTGGA
                     AVAAGIVSLGFGISGVAAACGSADSPCGGAGGQPKYYSNSNALGSAFAALGAVLLVGG
                     GAVIAFPEPVQQKDAYVLTIRQ"
     gene            7513..8775
                     /locus_tag="PKL17_17915"
     CDS             7513..8775
                     /locus_tag="PKL17_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46664.1"
                     /translation="MLNKAVGFFGLALALSGCSDYWYTYHVLDSNADAGTDAAVSACL
                     SGEPYEFVDGVYKTCCDPNDKAKSASNLQKTIELTQATPDSTSVTIKLGCSVPVQLTL
                     QKLASTTAAGEGSMLPIIARETIIDGNGSTLDAGLNERHFLVKPNVGLTLKNIKLIRG
                     RATAIPDATSARWPICVEGGPKLPADASCGGSILSFGRLTLDKVFITESRSENPTVAA
                     GGAICIAGSKLTLKNSIIYGNTSVSGDAVSRAEGAGIYLLSSDAEFTFSTILSNLNNT
                     KNTGPAAAIQFTGGSQKIFTNNNSLMVNYYAIATNNNQTANYALLIPCGLTANSLSTA
                     AFDKMLKPPTCPGSLMGYGSSLNISGTGADWPSPSMYPAVISPSGTFDGVLIKSTDPA
                     SCASAGADYFGNTRKASGCMPGAVELRW"
BASE COUNT         1806 a         2457 c         2858 g         1783 t
ORIGIN      
        1 tgacctacat cgacggcaag cgctgctggg tcaacaaggt catctacggc gacgcgagcc
       61 cgacggccat caatccacgc cccgatggaa aacggcagct ctttgccgcg ggcgactcga
      121 acaccgacgt gacgtttgtg cgcgacgcgg aaaagctcaa gctggtgata aaccgcaaca
      181 aagccgaact gatgtgcaac gcctacggaa acttcggaaa ccgctacttg gtcaatccga
      241 tgttcatcga tcccaagccg cagcagacgg cgctgtatcc gtgcacgacg accgcttgcg
      301 taaattcaaa tgggatggcg acgccctgcc tcgacgaagg ccagggtccg atcgcggatc
      361 aaaaagacgc cgtctacccg atgtaacagt cctgaaacag gcgcgggctg ggattgcact
      421 tctcgaagtg agtaagtaga gtccgcgccg tcatgacagc cgatcgccaa taccagtccg
      481 ttagctatcg tccctgtgaa ctcgaacatc gctacggtcc gtcggtgcac atcctgcgtg
      541 atccactggc gctgtcgctt ttggccaagc tgtgtcaccg cgatgtggtc cagcccgagg
      601 tcagtcgact catcgtcgag ctgtaccggg tgctggtgca cgaagtggtc gcggcggagt
      661 ttcctcgtca gcagtcgagc attccgacgc gcatgattca gtacgtgccc gaaggtgtct
      721 acgacggtga agtgatcgcc gaaagtacgc cggcagtggt ggtggcgctg gcgcgggcag
      781 ggcttctgcc gtcgcaggtg agctatgact ttctcaatca agtgctgtct ccttcgggcg
      841 tgcggcaaga tcacctggcg ctgggtcgga cgctcgacgg ggcagggcac gtcaccggcg
      901 cgggactgta tgcgtcgaag atcggtggac cggtcgatgg tgcggtgatg ctgatacccg
      961 atccgatggg ggcgactgga tcgacggtga gccgggttct gcgacactac gaaaaagaag
     1021 tcgtgggcag gccggccaaa gttgtggcgg tccacttgat cgtcacgccg gaatacctgc
     1081 ggcatgtgac gaaaaaccat ccagaggttg tggtgtacgc gcttcggctc gatcgcggtc
     1141 tgtcggaacc cgatgtgctg gagacggtgc ccggcacgca ctgggatcgc gagcgcggcc
     1201 tgaccgacca ccagtacatc gttccaggtg gtggtggcct gggcgaagtg ctcaacaact
     1261 cgtttgtgta aatccccctc ggaacaggag cctatcgtgt cggaccaaga taccttcgtc
     1321 gaaccgaagc tttcgccgct tttcaccgag gagcagattg ccgcgcgcat tgccgaactg
     1381 ggggcgcaga ttgccgccga ctatcagcgc cgacatctga cgctggtgac ggttctaaaa
     1441 ggcagttttg tgtttgccgc cgacttgtcg cgggcgattc agcgggcaca ggtcgcgcaa
     1501 ggactgcccg gatcgacggt gacatgcgaa tttctgggac tttcgagcta cggcgacgaa
     1561 accgtgactt ccggcgttgt gcggatcacg tcggacctga gcaatcccat cgaaggctcg
     1621 gacatcctca tcgtcgagga catcgtggat accggcctga cgatgagcta cctgctcgaa
     1681 aacatgcgga cccgccaccc ggcgtcgatt cgcatctgtt cgcttttgca caagccgagc
     1741 cggaccaaaa aacgggtgcc catcgactac atcggcttca ccatcgagga tctgtttgtg
     1801 gtcgggtatg ggctcgacta caagcagcgc taccgcaacc taccttggat cggcgttctg
     1861 tcgctctaag acgatcactt ttggcgcact cggcgcgcgt ttcagacgaa aaccaagcta
     1921 cgtcgaaggc gcgggaatct gttaacatgg cagcaggatt cccatgtcga accaagcgcg
     1981 tccgaaggac gctgctgtct gtgcggactg tggacacgtt ctcgaagagg acatgccaca
     2041 tatctgccag ggggccgcga agaagtccgc gttcgacagc acgatcgggc ctggggatca
     2101 tccgacgatt cctggggaag tgtcttctgc cgatatccac gatccggcgc aagagctgat
     2161 cgggaaaatc atcggcgagc gctacgaaat ccagcgactt ctgggtcagg gtggcatggg
     2221 cgtcgtctat caggcgcgcc acatttcgct cgaaaagctc ctcgcggtga agctccttct
     2281 catcgcgcaa aacgaagagt atcagcgacg atttctccaa gaagcgcagc tcgcgtcgca
     2341 gatcaaccat cccaacacag tgtttctgtc tgactttggc gtgctgcctg acgggcgctc
     2401 gtacatcgtc atggaacttt tgcgcggtcc gacgctggct tcggtgatag ccgaaggcgc
     2461 gatggaaccg atgcgcgtct gcaaaattgc cgcacagatc gcccacggac tccaagccgt
     2521 ccacgaccag ggaattatcc accgagacct caagccggaa aacgtgtttc tcctcgagca
     2581 ggacggcaaa aaggacaatg tgaagattgt cgactttggc attgccaagg cgacgagcga
     2641 agcgccggtc gtgcgcaagc ggcactcgca ggaagacatc agccaggtta cggaacagaa
     2701 aatctcgtcg cagacgatgc cggggacgct gatggggacg cccgcgtacc ttgcgccgga
     2761 gatggcgctt gggcaagagg tggactttcg cgtcgaccag tatgcgctgg gcgtgatcct
     2821 gttcgagatg ctgactgggg atctgccatt cgaggcgacg acgattgggc agatgctcca
     2881 gcatcacatt tcgacgaagc cgccgctgct gagccggaaa atttccgata gttcaccgac
     2941 gctggatgcg ctggtggcgc ggcttttgtc gaaaaagccc gtcgagcgct ttccgagcat
     3001 gcgagcggtg gccgaggcgc tggaaaaaga agtcgagctg cgactgctcg cgcgggggga
     3061 tacggcgctg cctgcccaca ttgccgaggc gctgagcaaa acccaagcgg gcacgcacat
     3121 ccgcatcgcc ggtcgcaaag tgccgctgtg ggtgctcttg ccggtgctga tgattttcgt
     3181 cgttgctggc ggtgcggcaa gctggctgct ttggcggcag cgcgtcgaac aagttggcat
     3241 cacacccgaa gagctggccg agctgcgcca gaaagcgctc gacgtggtgc gtgaatatgc
     3301 caaaggcgac gatgcaccgc tgcgggcgct gtcggcacag gcgctgtcgc agacccgcga
     3361 tcccagccaa aggccgcagc ttgaagcact gcttggggac gcggcaccgc aggtgcgggt
     3421 gcgggcggcg ttggcgctgg gggccgttgg tgacaggcaa gctacgtcgg cgctgcgcgc
     3481 agcactttcg ggtccgatcg aggtcaagct ggccgcggcg atggcgctgt cgcagctcgc
     3541 agattcagac ggacacaagg ttctggtcga cgcgcttgcg gccaaagaac cggcggtgca
     3601 gaagcaagcg gcgctgtatc tgtgcgagct gggcgacgaa gcggcgcggc aggtgctgtc
     3661 ggatttgcac aaccgcagtg ggctgggcga cgacgaggac tttttggcgc tccagtgtct
     3721 ggtgcggttt ggcgatgaac cggcgcgcgt cgaacttgca gccaaagtcg aggctggaca
     3781 aaatcgcaag cagaagctcg aagcgatgtt ccgtctggcc gaactgggtg acgagccgac
     3841 gcgcaccgag ctgcgtcaga tcgcgagcaa gcgcggcggt gaacagcttt tgaccgccaa
     3901 tcgcctcgcg agtcccgagg aaccgcagct gctcgcggta tttcgtccga ttttggccaa
     3961 tgagtttggc gtgccgacgg ggcgtcagct tgccgccgag ggtgtgggca agagcggcga
     4021 actgccccaa atcaaagagc taggcccgct gctcgacgga aaggtcgttg cggtggtgcg
     4081 acagacggcg gcggcagcca ttttgcgcct ggcgagcggc gatccggggc ttttgacgac
     4141 cgccagcctc agctgggcac aaaacgagct gggttcggga tcggcgtcgc ggcggctttt
     4201 ggccctgcaa gtgctcggcg atttgccgtc gagccaggcg gtggcgctgc tttcaacggc
     4261 gatgcaagat ggctcggcgg acattcgtca cgcggcggcc aaagcgctgg gccgacggaa
     4321 agagcggacg gcgatgacgc tgctgctcga aagcgtcgcg gatcgtgacg aaaaagtgcg
     4381 ctatgcgtcg ctgctcgcgc tgctcgatct ggtcggacgg ctgtcgaaac gcgatgccgc
     4441 cggactattg caagctgccg agggtgtgct caaagagatt ttgcaaaaag gatcggtgct
     4501 ggaacaggcg ctcgcgtcgt cggtgctgct gcggctcggc gataagaagc aagtcgatcg
     4561 actgaaagcg ctgcgatccg atcacgatcc gcagattcga catctgattt tggatgcgct
     4621 gtcgtcggac catgactttg tggcgctgtt tctaagcgac gcaattgatt cagtgcggct
     4681 gcatgcggcg cgaattttgg cgcagtcggg cgacaagcga gcggtgccgg tgctaaaggc
     4741 gtttttttcc gtcggtggtg tcgatggact gcatgcggcg gggcttctgc gtcggctcgg
     4801 aattcgggaa aacctgccgc cggatttgct gcggcttctg cactctgcgt ccgtcgagga
     4861 acgtgttgcg gcggtgcggg cactgcgcga tgtggatgtg acgctggcgc tttctttgct
     4921 ccgaccggcg gcggcggacc cggctgccga ggttcgtcgc gctgtcgccg aggtggcttc
     4981 gtctctgccc aagggcccag gcggctacgc gggggttccg cttctgcgtc agctttcgca
     5041 agacaacgaa gcgacggtgt ggacgctggc gcgatcgctt ttggcgcggc ttttgacgcc
     5101 ggaagatgcg ccgattgttg aaccgacgct gccgccacga cgctcggcgt ttgcccccga
     5161 accgcccaag ccggcggcga tggaaaccga accgcccacc ggcacagtga cgggcaagct
     5221 gtccgtcgtg gcagcagagc cggtggtgtt tcaagtcggc aagcgtcctt ggcagcaggc
     5281 accagcgacg gtggaattgc cggttggcaa gcacatggtg gcgaccttgg cgacggaaca
     5341 ggaagtgaac atcgtcgcgt cgggggaacc atacaagctg gagcttagcg agtctccggc
     5401 ggaaaagttt ctgcggatcg gcatcgcggc ggccaaaggc ggcgacagca aaaaagcgct
     5461 gaccaacctc gagcgggcgc gggcgatctg tcaaaaagac cgcaagcaca gccacgatcc
     5521 gccctgtcac tcgattgtgt tcgacgcttc gtactaccag ggtcggatgc acgaaaacgc
     5581 caaagaccta cacctggcga tggaggcgta tcaaaaagcg cttgagcaag gcgaaaaggt
     5641 gaaaggtcgc accgacctca agaaccagtt ggtagagatc acggggcgac tgcgggggcg
     5701 gcttgggcaa gtgatcgtaa aatcgctcgt caacgggcgc tgccagagca aggtgtactg
     5761 gatgattccc gccaaacatc aggttcgcga cggacagggg cgcagcattt cggtcgatct
     5821 gcgcgccggc gaccagcgcg aggtgggaag ctgcgacggg gataagtagc aatgagttgc
     5881 gttttccgtc ggaattcgag gttgcgcaaa gcggtgctgc tatcgctcat cgggcacctg
     5941 cttgtccctt cgttttccgc cggaaagtcg cgcagcacgc cgcagattca aaaacgcagt
     6001 cggcaagatc aggaagtgtc ggcgcagctt gtcaaagacg ccgaaaaagc gatcaccgaa
     6061 cagaactggg tggatgcaca aaggctgctc accgacgcat atctgaagtt tccggggccg
     6121 cagattttgt atcacctggg cgtcgtggcg gcgaaagagg ggcggctgct cgacgcgcac
     6181 gatctgctgc gtcgttacga gtccgatccg gcgagcacgc cgagccaggc gatgaccgcc
     6241 gagattcagc ggctgctggc caaacagcgt ccaccgagcg gcaaagtgct ggttctgggc
     6301 gacgacgggg cgattgtgcg ggtcgacggg cgcgtggtcg gttcgctgcc gcttgtgcag
     6361 ccacttcttg tgtcaccggg ggactcgcac accatcgcgc tcgattttcc cgaacagcag
     6421 atcccggcgc aggtgacgat ctcggcgggc cgttttgccg aacttcggat aaaccgagcg
     6481 acgcgcgcgg tgcttttgac ggtgctgccg gcgtttgtgg tgctcagcga ataccgcgat
     6541 gtgccgcagc cgtcgatggt caagctcgac gaagcgatcg aagccggtgt gcagggcgaa
     6601 aaaaagtcgg ttctccgtcg agaaattgcg cttcgccaag cgcccgagct ggcttcgtgc
     6661 ttggatcaga tcgactgcca agcgaagctg gcggaaaaaa ctgaagtcga tgggctgatt
     6721 cggctctcgg tggaaaacac cgcggcgcgg accggtggtg ctccgtcgta caagctgacg
     6781 ctacagcttc tcgacccgac ggttggtgcg atcgccggcc aagccagccg cgagctgccg
     6841 cccgagcgcg ctacgacggc gatgactgaa atgctgtcgc agatcattgg tgatgcggcg
     6901 ctgcgtcggc gtggtctgat cagcgttcga tcgaatcccg acggggccga agtgtatgcg
     6961 ggtgaacgca tgctcggcaa aacgccgttt cagcgtgccg cgtgggtcgg tcgctacgat
     7021 ctgacatttc gcaagccagg ctttgcgccc gaatcggcgc agacggaagt gaccgacggg
     7081 aaaaccagcg agctggaagt cagcctaaag cccggagtga cgctgccacc accgccgcca
     7141 ccgtcgcgtt ttgactttgt atttacgccg cacgactttc gtccgcctcg cccgttttgg
     7201 cgctggctca ccggcggtgc cgcggtggct gccggaattg tgtcgcttgg atttggcata
     7261 agcggcgttg ctgctgcttg tggatctgcc gatagtccat gtggcggggc cggtggtcag
     7321 ccaaaatact atagcaatag caatgcgctt ggttctgcgt ttgctgccct tggcgctgtg
     7381 ctgctggttg gcggtggtgc agtaattgca ttccctgaac ccgtgcaaca aaaagatgcc
     7441 tatgtgctaa ctattcgtca gtaagtgccc gatgtgttgt acttgctgaa tctgaaaaat
     7501 agggggtcta aaatgcttaa taaggccgtt ggtttcttcg ggcttgcgtt ggcgttgtct
     7561 ggctgctctg attattggta tacatatcat gtgttagata gcaatgcgga cgcaggaacg
     7621 gatgctgcgg tgtccgcatg cctgagcggt gagccttatg aattcgtcga cggtgtgtat
     7681 aaaacttgct gtgatccgaa tgacaaggcg aaatctgcgt caaatcttca aaagacaatt
     7741 gaactaacac aagcgacgcc agattcaacg tcagttacaa taaaacttgg ttgcagtgtt
     7801 ccggttcagc ttaccttgca aaagctggct tctacaacag cggctggcga gggatcgatg
     7861 ctgccaatca ttgctcgcga aaccatcatc gatggcaatg gttcaacact cgatgcgggt
     7921 ttgaatgagc gacattttct ggtcaagcca aacgtcggac tgacactgaa gaacatcaaa
     7981 ttgattcgtg ggcgtgcgac agcgattcct gacgcaacga gcgctcgctg gccgatctgt
     8041 gttgaaggcg ggcccaagct tccagcggac gcctcatgcg gtggctccat cctttcgttt
     8101 ggacgactga cgctggacaa ggtgtttatc accgaaagtc gatccgagaa tccgactgtg
     8161 gctgccggtg gtgcaatttg catcgcaggc tcgaaactga cgctcaaaaa ttcgatcatt
     8221 tacggcaata catcagttag tggtgatgcg gtttcacgag cggaaggtgc aggcatttat
     8281 ttgcttagct cggatgccga atttacattt tccacaatat tgtcaaattt gaacaacacg
     8341 aagaacacag ggccggcagc cgcgattcaa tttactggag gctctcaaaa aatcttcaca
     8401 aacaataata gtttaatggt aaactattat gctattgcga caaataacaa tcagactgcc
     8461 aattatgcgt tgctaatacc gtgtggtttg acagccaaca gtttgtcgac cgctgctttt
     8521 gacaaaatgc tcaagccgcc gacatgtccc ggaagtctga tgggatatgg gtcaagtctc
     8581 aatatttcgg gaacgggagc agactggcca tccccatcca tgtatcccgc cgtaattagc
     8641 cctagtggta cattcgatgg cgtactcatc aagagtaccg atcctgcgtc ctgtgcaagt
     8701 gctggagctg attattttgg caatacgcgc aaagcgagtg gttgcatgcc gggggctgta
     8761 gaacttcgct ggtagcgcgt tggttttgcg ccatcggcgc gtgtgccgcc gggattcttg
     8821 cggcggcggc gggctactct gcttttttgg ggcagggttt tttgccaagc tcgacggctt
     8881 tttggttctg cggatcgacg tcga
//
LOCUS       DAORGN010000207         8855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3500_length_8855_cov_6.523750, whole genome shotgun sequence.
ACCESSION   DAORGN010000207 DAORGN010000000
VERSION     DAORGN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_8855_cov_6.523750"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKL17_17920"
     CDS             complement(<1..573)
                     /locus_tag="PKL17_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46665.1"
                     /translation="MSRLHLTLPLCSLGFFGCDNPSPTDQLPVEPALSTCSLALPDPQ
                     TKSTASAEVAGASACLSVHPSEAAQTPMPLVAVRKGQDLSLDGYKTLGPAVMLQLGQP
                     VGQRGVDVTLPFAFLQIPENARTLRHRLVVLSRFGQSKAHVTAVENITVSTANGGQLR
                     FHLPGHGVSPLGPEKTLSDVATFQVAMPTDL"
     gene            644..1084
                     /locus_tag="PKL17_17925"
     CDS             644..1084
                     /locus_tag="PKL17_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46666.1"
                     /translation="MESVTLSDGLQAGPLSLLVQGVLSDTARRYGDRLPGLHSRLGLV
                     AIDSQEEITLVFSDERCLILPGLVEPDLTFEADSELLPRLQTVPTVLGIPIFASPQGF
                     ELGLSLLRHPLKVRGLSLLLVNPARAARALVDAAKLVKLLAGTY"
     gene            complement(1098..2576)
                     /gene="selA"
                     /locus_tag="PKL17_17930"
     CDS             complement(1098..2576)
                     /gene="selA"
                     /locus_tag="PKL17_17930"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNK46667.1"
                     /translation="MSEQQSLRRLPKVSAVLAQQDVADLVGKLPRWAVVQAVRDELAA
                     LRAQLASGAAPATMTGDREGKIPMSAQTIETRARLLCASPLRRVVNATGVVLHTNLGR
                     APLAAPAIARIFELAGGYCNLEYQLDSGQRGSRQQPLGKLLRDLTGAEAHLVVNNNAA
                     AVLLALAGLCQGREVVVSRGELVEIGGSFRVPDVMRASGAFLREVGTTNRTHPRDYVS
                     ATGEATAAYLKVHRSNFAQVGFVTDVDVKTLADLAHQRGLLCLYDLGAGALASVPRAV
                     KTSSASDQEFALADFAGALAQPEPTVAEVVAQGVDLVMFSGDKLLGGPQAGILCGKAE
                     VIAKLAKQPLLRALRPDKLTLLALVATLELYRDGRASDIPTLQMLQTSEDELARRAEA
                     LRSLIEARGGTATTVRVRSAVGGGSQPLHKPWSWAVSPVLAEDEVLPAAAALRRHHLP
                     VIARVSAGKLLCDVRTIADADFPAVADAVCEAMKTLPMIGGR"
     gene            complement(2569..3414)
                     /locus_tag="PKL17_17935"
     CDS             complement(2569..3414)
                     /locus_tag="PKL17_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNK46668.1"
                     /translation="MQPLSFHLRVAPFAPTSRPLVLGHRGASHDHPENTLLAFREAMK
                     QGADGVELDVMRCGSGEVVVVHDDDLQRLAPGQPGADGLVRQMSLGDLRRFDVGRGER
                     IPTLEEVFLALADTALVNVELKSPTLHRSADYLKLPFDDGLAAAVGAVLRRCGRLDPS
                     GRTLISSFDPFQLLRFQTVAPGLPLGYLFEAGQGGFFRGLHKLPLFRLAAVHPEAKLL
                     DGPSLRAWKARGLRVNTWTVDDPREVAAVAMLGVDAIITNRPAATLAALTKREAATQK
                     DPADE"
     gene            3478..7089
                     /locus_tag="PKL17_17940"
     CDS             3478..7089
                     /locus_tag="PKL17_17940"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK46669.1"
                     /translation="MSSDDPRIGSVFGNFRLLRKLGEGGMGVVYEAEHHTIGRRAAVK
                     VMHAEFAKNDEYAKRFLNEARAVNIIRHPGLVEIFEFGQEQDGSLYIVMEFLEGKSLY
                     DRYVQPGTKPKPIEAARLGEQAARALAAAHEKHVVHRDLKPENLMLVPDPVRPTEDRV
                     KILDFGIAKVSRKAASTAPPTRESGKRGVRTGAGSYLGTPLYMAPEQHGGAEDVDGRA
                     DVFSFGLVLYELLTGKLPYETNSLALIAKPATMVHELNKDVPLKLSQLIAQMMAMDPT
                     QRPLMSEVASQLGALVKQSKRRISPLLTALGGAAFVIVCAVTAFAIWRVVHVPTLAEA
                     RERALKVVQSGLSASDPSERSLALRALELSHDPGFSNLAQPLLGDGQPTVQASAAKTI
                     GELGAVELQPDLVATLDKSQTPKTTIEVAAALAKLSHPRGPETLRSLLQNADELTKIE
                     SALRLAEQGEPGAMALLQKATEGEPQPTMLPLLLALVRGGDSQAAGKLRQLFDGKAPA
                     AEKPRIAFQLARLGDGSARDWLEKTAQSDGPEQVKATRFLAQLGDVSGAALLEKSSID
                     DKQPDNTREVAVEGLADCGKSEDASRLALVLGERNIGRRLRYTTAGAILLLATGNQLP
                     PDELSLGWAKSALRSNSESTRELAVLLLGSQSSGASLDALRNAMTDKSTTVRKSAVGA
                     LAERGGKDALLALQAGLSDGEKDVRVAAAEAIGRVSSAMIGRGEADAARPMAEKVSKL
                     LQSSDERERMAASSALLRMGDRSQAAVVRSGMQSADATVRRQSIEQGEVEDEALQKAL
                     EDSDGKVRFAAAKKLAQKGSKLAIPVLKETASSSELDAVVAYKLLHKLGEEVEPPPQS
                     VLVGASTAERHQILSTVAELPPKAAKKLMPSLATDSSPVVRRRAAEVASVLYQRSKDA
                     AFLPTLRALRADSDPLVRTRANQLLTDLAKQNPQVLTEKPLQEPVEKTAKTKPPVQNP
                     TTEPANPDAGTKPVENPASTGTGFVQLIGEEGVRVQIDKGPAQLLSEKPISLPVGKHR
                     ISTSSGSQEVQVSDGATLTVKVSGSLAEQLTHDAAESLRNKDLTKAQSLIERARRVGI
                     RTGARPQLLSELIYLQAKIYDARGQWREAMNEYEKYQSLPPAHQRAETLSAVRAAVAK
                     LAPRMGLIQIFTLVNGKCKLTEKHYLPPGEHLISLGGGKSKVMSIYAGVTTPVRQCP"
     gene            7080..8681
                     /locus_tag="PKL17_17945"
     CDS             7080..8681
                     /locus_tag="PKL17_17945"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46670.1"
                     /translation="MPVTSRHKRLDWACLGSVTAVLAHSVPALAVPAPVASKPAAQAR
                     FPAKPPTGPFAERTAKLIAEGRAALEAKDFPKAQRSFEQAYWFRSTPDLLYLLGQVAD
                     AQGQNVTALDFFRRYLESGSQSTDEATTEALRKRIQEVPATSSEVTISGHGGGLLSVD
                     GRLLGVLPLHRALLLTPGPHRFKIEWKGAHFESDPLLIPESRTAELHLTPGTAGSLIA
                     VLTLNSFAVLKLSSTDLPPEQHKTVEESITTAARNEHTVFLPDAKLRELLQAEPQGCL
                     DDTPCLDRIASKAEIRYVVRVALSGQQSAPTVAAAAELVDVASGLAAGRIEENLPRDS
                     LGPQTVALIRRLFQTALGRPRGMLHIESTPSESIVRIDDRVLGKTPLERVSLAGKHRL
                     HVEQGGFVPFDSDITLEPGQTAQIAATLTAKEQPPPPPPPEFVTIPGESSRSRIARYV
                     LGGAASGLGVALVGLGGWALSQNGQCGDNPAPQNGIPCELVYNTSTVGRLLIGSGIGL
                     TVGGSLLMLLPGRRPKQVLKSALTP"
BASE COUNT         1858 a         2765 c         2683 g         1549 t
ORIGIN      
        1 cagatcggtc ggcatcgcca cttggaatgt cgccacgtcg gacagcgttt tttctggccc
       61 aagcggcgat acgccatgcc ccggcaagtg gaagcggagc tgaccgccgt ttgccgtcga
      121 cacagtgatg ttttcgaccg ccgttacatg tgctttcgac tggccaaagc gcgacaggac
      181 gaccagccga tgccgcagcg tccgcgcgtt ttccggaatc tgcaaaaacg caaacggcag
      241 cgtcacatcg acaccacgct gaccgacggg ctgcccaagc tgtagcatca ccgctggacc
      301 cagcgtctta tatccatcga gcgaaagatc ctggcctttg cgcacagcga cgagcggcat
      361 cggtgtctgc gcggcttcgc ttggatgaac gctcaggcag gcgctggctc cggccacctc
      421 ggcactcgcg gtgcttttcg tctgtggatc gggcagcgcc agactgcacg tcgaaagcgc
      481 tggttcgacc ggaagctggt ctgtcggcga tggattgtca cagccgaaaa aacccaagct
      541 gcacaaaggc aaagtcaggt gcaaacgact cacggtccga cgagaaaaca actttcgtgg
      601 cgaggatgga atttgcattc cgcggagtgt agcctgaagt gatatggaat ccgtcacgct
      661 ttccgatggc ttgcaagcgg gtccgctttc gctgctcgta cagggcgttt tgtccgatac
      721 ggctcgacgg tatggcgatc ggctgccggg gctgcacagt cggctggggc ttgtggcgat
      781 cgactcgcag gaagaaatca ccctggtttt ttccgacgag cgctgtctga ttctgccggg
      841 acttgtcgaa cccgatctga ccttcgaggc agacagcgag cttttgccgc ggcttcagac
      901 cgtaccgacg gtgctgggga ttccaatctt tgcgtcaccg caagggtttg agctggggct
      961 gtcgctcttg cggcatccgc taaaagtgcg cggcctgtcg cttttgctgg tcaatcccgc
     1021 gcgggcggcg cgggcgcttg tcgacgcggc aaagcttgtg aaactgctgg cgggaacgta
     1081 ctaaaaaacg aacctgctta ccgtccaccg atcattggca gggtcttcat cgcttcgcac
     1141 acagcgtcgg caacggcagg aaagtcggcg tcggcgatgg tgcgcacatc acacaaaagc
     1201 ttgcccgccg acacccgagc gataactggc agatggtgac gccgcagcgc cgcagcagcg
     1261 ggcagaactt cgtcttcggc cagcacaggc gacaccgccc agctccacgg tttgtgcagc
     1321 ggctgcgaac ccccaccgac cgccgatcgc acgcgcacag tcgtcgctgt gccaccgcgc
     1381 gcttctatca gactgcgcag cgcttcggcg cgccttgcca gctcgtcttc ggatgtttgc
     1441 aacatctgaa gcgttggaat gtcggacgcc cggccatcgc gatacagctc cagcgtcgcc
     1501 acaagcgcca aaagtgtcag cttgtcaggc cgcagcgcgc gcaaaagcgg ctgcttggcc
     1561 agctttgcaa tcacctcggc tttgccacac agaattccgg cctgcggtcc acccagcagc
     1621 ttgtcgccgg aaaacatcac cagatcgaca ccttgcgcca cgacttctgc gacggttggc
     1681 tcgggctgcg ccaaagcgcc ggcaaaatcc gccaacgcga actcttgatc cgacgctgag
     1741 ctggttttga ccgcacgagg caccgaagcc agcgccccag cgcccaaatc gtacagacac
     1801 aaaagaccgc gctggtgggc caggtccgcg agcgttttta catcaacgtc ggtgacaaag
     1861 ccgacttggg caaagttgct gcgatggact ttcagatacg ctgcggtcgc ttctcccgtc
     1921 gcagatacgt aatctcgcgg atgggttcgg ttggtcgtac ccacttcacg caaaaaagcg
     1981 cccgacgccc gcatcacatc cggcacgcgg aaagaaccgc cgatttcgac cagctcgccg
     2041 cgcgacacca cgacttcccg tccctgacac aaaccggcaa gcgccaacag aaccgccgct
     2101 gcattgttgt ttaccaccaa atgcgcctcg gctcccgtca gatcccgaag cagcttgccg
     2161 agcggctgct gccgactgcc gcgctgaccc gaatcaagct gatattcgag attgcagtag
     2221 ccaccagcca gctcgaaaat ccgcgcaatc gcaggcgcgg caagcggcgc ccgcccgagg
     2281 ttcgtgtgaa gcaccacgcc cgtcgcattg acgactcgac gcagcggcga tgcacacaga
     2341 aggcgagccc gcgtttcaat ggtctgcgcc gacatcggga ttttgccttc ccgatcgccg
     2401 gtcatcgtcg ctggcgctgc acccgatgca agctgcgcgc gcaacgccgc aagctcgtcg
     2461 cgaacggctt gcacgactgc ccagcgcggc agcttgccca ccagatcggc cacgtcctgc
     2521 tgtgcaagca ccgcgcttac tttgggaagt cgcctgaggc tctgctgctc actcatcagc
     2581 tgggtctttc tgcgtcgcgg cttcacgttt cgtcagggcc gcaagcgtcg ccgcaggccg
     2641 gttggtaatg atcgcatcga cgcccagcat cgccaccgcg gccacttcac gcggatcatc
     2701 caccgtccag gtgttgacgc gcagacctcg cgctttccac gcacgcaggc tcggtccgtc
     2761 gagcagtttg gcttctggat gcaccgcggc gagccgaaac agcggcagct tgtgcagccc
     2821 acgaaaaaaa ccgccctgcc cggcttcaaa caagtaacca agcggcaggc ccggtgccac
     2881 cgtctgaaaa cgcagaagct gaaacggatc gaacgaagaa atcagcgtgc gtccacttgg
     2941 gtcaagccgc ccacagcgcc gaagcactgc acccaccgcc gcggcaagac cgtcgtcgaa
     3001 cggcagcttc agataatccg ctgatcggtg cagcgtcggt gacttgagct cgacatttac
     3061 aagcgcagtg tcagcaagag caaggaagac ttcttccagc gtcgggatgc gctcacctcg
     3121 accgacatcg aagcggcgca gatcaccaag cgacatctgg cgaaccaatc catccgcgcc
     3181 cggctgtccc ggtgccagcc tctgcaagtc gtcgtcgtga accaccacga cttcacccga
     3241 tccgcagcgc atcacatcca gttccacacc gtctgcacct tgtttcatcg cttctcggaa
     3301 cgcgagcaag gtgttttcag ggtggtcgtg cgacgcacct cgatgtccca gcacaagagg
     3361 gcgtgacgtt ggcgcaaaag gtgcgacgcg cagatggaaa gacagaggct gcatattggg
     3421 aagaagatag actaagctaa gttaggcgtt ttttagcaga cgacgcctgc tcagggtatg
     3481 tccagcgacg atccgcggat tggtagcgtg tttggcaact tccgtcttct gcgcaagctc
     3541 ggcgaaggcg gaatgggcgt cgtctatgag gcggaacatc acacgatcgg tcgacgagcc
     3601 gccgtcaaag tgatgcatgc cgagtttgca aaaaacgacg aatacgccaa gcgctttctc
     3661 aatgaagcgc gggcggtgaa catcattcgc caccctggtc tcgtcgagat ttttgagttc
     3721 gggcaagagc aagacggctc gctgtacatc gtcatggagt tcctcgaggg gaagtcgctc
     3781 tatgaccgct atgttcagcc gggaacaaag cccaagccta tcgaggctgc gcgacttggc
     3841 gaacaagccg cccgcgcgct cgccgccgcc cacgaaaaac acgtcgtaca tcgggatttg
     3901 aagccggaaa acctgatgct ggtgccggac ccggtccgtc cgacggaaga tcgagtaaaa
     3961 attctcgatt ttggcattgc gaaagtgtca cgcaaggctg catcgacggc acccccgacg
     4021 cgggaatccg gcaaacgggg cgttcgtacc ggcgcaggtt cttatctggg cacgccgctt
     4081 tacatggccc ccgagcagca cggcggtgcc gaagatgtcg acggacgagc ggatgtgttt
     4141 tcgttcggtc tggtgctgta tgaactgctg acgggaaagc tcccctatga aaccaactcg
     4201 ctggcgctga ttgcgaagcc agcgacgatg gttcacgagc taaacaaaga cgttccgctc
     4261 aagctgtcgc agcttatcgc gcagatgatg gcgatggacc cgacgcagcg tccgctgatg
     4321 tcggaagtcg caagccagct tggcgcgctg gtgaagcaga gcaagcggcg gataagtccg
     4381 cttttgaccg cgctcggtgg tgccgccttt gtcatcgtct gcgctgtgac ggcttttgcg
     4441 atctggcggg tggttcacgt cccgacgctg gctgaagccc gcgagcgcgc tctcaaagtg
     4501 gtacaaagcg gtctttccgc cagcgatccg tcggaacgga gcttggccct gcgtgctctg
     4561 gagctgagcc acgatccggg tttttccaat ctggcgcagc cgctgcttgg ggacggacaa
     4621 ccgacggtgc aggcttcggc ggcgaagacc atcggcgagc tgggtgcggt cgaacttcag
     4681 ccggatctcg tcgcaacgct cgacaaaagc cagacgccca agacgaccat cgaagtggcc
     4741 gccgccctgg cgaagctgtc ccaccctcgc ggacccgaga cgctgcgctc gcttttgcaa
     4801 aatgccgacg agcttacaaa aatcgagtcg gcgctgcggc tggcggaaca gggcgaaccg
     4861 ggtgcgatgg cgcttttgca aaaagccacc gagggtgaac cccagccgac gatgttgccg
     4921 cttttgcttg cacttgtgcg cggcggcgac agccaagcgg cgggcaagct acggcagctg
     4981 ttcgatggaa aagcgccagc agcggaaaaa ccacggattg cttttcagct ggctcggctt
     5041 ggtgatgggt cggcgcgcga ctggctcgaa aaaacggcac agagcgacgg accggaacaa
     5101 gtcaaagcga cccgattttt ggcgcagctt ggcgatgtga gtggggccgc gctgctcgaa
     5161 aagagcagca ttgacgacaa acagcctgac aacacgcggg aagttgccgt cgaaggactg
     5221 gccgactgtg gcaaatcgga agacgcgagc cgcttggcgc tggtgcttgg tgaacgcaac
     5281 attggccgca gactgcgcta caccaccgcc ggcgcgattt tgctcttggc gacgggaaac
     5341 cagcttccgc cagatgaact ttcgctcggc tgggccaagt cggcactgcg ttcaaacagc
     5401 gaatcgacgc gagagctggc ggtgcttctc ctcggaagtc agtcgagtgg tgcaagcctt
     5461 gacgcgctcc gcaatgcgat gacggacaaa tcgaccacgg ttcgcaaaag tgccgtcgga
     5521 gccctggcag agcgtggtgg aaaagacgcg cttttggcac tgcaagccgg tctaagcgac
     5581 ggcgaaaagg acgtgcgcgt cgctgccgcc gaagccatcg gtcgagtgtc atcagcgatg
     5641 atcggtcgtg gagaagctga cgccgcacgg ccaatggccg aaaaagtcag caagctgttg
     5701 cagagcagcg acgaacgcga acggatggca gccagctcgg cactgcttcg catgggcgat
     5761 cgcagccagg ccgccgtcgt gcgcagcggc atgcagtcgg cagatgcaac ggttcgtcgt
     5821 cagagcatcg agcagggcga agtcgaagac gaagcgctgc aaaaagcact ggaagacagc
     5881 gacggaaaag tacgatttgc ggcggcgaaa aaactggcgc aaaaaggcag caaactggcg
     5941 attcctgtgc tcaaggaaac ggcttcgtcg tcggagcttg atgcggtggt ggcatacaag
     6001 ctgctgcaca aactgggaga agaggtcgag ccgccgcccc agagtgttct tgtcggagcc
     6061 agcaccgccg agcgccatca aatcttgtcg accgtcgctg agctgccacc caaagccgcc
     6121 aaaaagctta tgccgagcct ggcgaccgat tcttcgccgg ttgttcgccg tcgggccgcc
     6181 gaggtggcgt cggtcctgta ccagcgcagc aaagatgcgg catttttgcc gacgctgcgc
     6241 gcactgcggg ctgattccga tccactggtc cgcacgcgag cgaatcagct gctgaccgat
     6301 ttggcgaaac agaatccgca ggttctgacg gaaaagccgc tgcaagagcc ggtcgaaaag
     6361 accgcaaaaa ccaagcctcc tgtgcaaaat ccgacgacgg aaccagcgaa tcccgatgcc
     6421 ggtacaaagc cggtggaaaa tccagcctcg acggggactg gctttgtgca gctcatcggg
     6481 gaagaaggcg tccgcgtgca aatcgacaaa ggtccagcgc agcttctgtc ggaaaaaccg
     6541 atttctctgc cggtgggcaa acatcgaatc agcaccagct caggcagcca agaagtccag
     6601 gtttccgacg gcgcgacctt gacagtgaag gtgtcggggt cgctggccga acagcttacg
     6661 cacgatgcgg cggaatcact gcgaaacaaa gacctgacca aagcgcagtc gctgatcgag
     6721 cgcgctcgac gggttggcat tcgcaccggg gctcgcccgc agcttctgtc ggaacttatc
     6781 tacctacagg ccaagattta cgacgctcgc gggcagtggc gcgaggcgat gaatgaatac
     6841 gaaaaatacc agagcttgcc gccagcccat cagcgcgccg agacgctgtc tgcggtacga
     6901 gccgccgtcg ccaaactggc accacggatg ggtctgattc agattttcac gctcgtaaac
     6961 ggcaagtgta agctgaccga gaagcactat ctgccccctg gtgagcacct catcagcctc
     7021 ggcggcggca agtccaaagt gatgtcgatt tacgctggcg tcaccacgcc cgtgaggcaa
     7081 tgcccgtaac ctctcggcac aagcgcctcg attgggcgtg tctggggtcg gtcacagcgg
     7141 tgttggcgca cagcgtccca gcgctcgccg tcccagcgcc cgtcgccagc aagcccgcgg
     7201 cacaagcccg ctttccggcc aagccaccga cgggaccatt tgccgaacgg accgccaagc
     7261 tcatcgccga aggccgcgcc gcgctcgaag ccaaagactt tccaaaagca caacgcagct
     7321 tcgagcaagc ctactggttc cgatcgacgc ccgacctgct gtatctgctg ggccaagtcg
     7381 ccgacgccca agggcagaac gtcactgcgc tcgatttttt ccgtcgctac ctggaatctg
     7441 gatcccagtc aacagacgaa gctacgacgg aagcactgcg caagcgaatc caagaagtgc
     7501 cagccacgag cagcgaggtc acgatttcgg ggcacggtgg tggcttgctt tccgtcgacg
     7561 gacgcctgct cggggtgctg ccactccacc gcgcactctt gctgacaccc ggaccgcacc
     7621 gcttcaagat cgagtggaaa ggcgctcatt ttgaaagcga tccgctgctg attcccgagt
     7681 cccgcaccgc cgagctacac ctgacgcctg gaaccgcggg atcgctgatt gccgtgctga
     7741 cgctcaattc gtttgccgtg ctgaagctat cgagtaccga cttgccgccc gagcagcaca
     7801 agacggtgga agagtcgatc acgacggcag cacgcaacga acatacggtg tttctgccgg
     7861 acgccaagct gcgagagctt ttgcaggcgg aaccgcaagg ctgtctcgac gacacgccat
     7921 gccttgaccg aatcgccagc aaagcggaaa ttcgttacgt cgttcgcgtc gcactttcag
     7981 gacagcaaag cgcacccact gtcgctgccg cggctgagct tgtggacgtg gccagcggtc
     8041 ttgccgccgg acgcatcgag gaaaacctgc ctcgcgacag ccttggccct cagaccgtcg
     8101 ctttgatcag gcggctgttt cagacggcgc tgggccgtcc ccgcggcatg ctgcacattg
     8161 aatcgacccc cagcgaatcg atcgtgcgca tcgatgaccg cgtgctcggc aaaacgccgc
     8221 ttgagcgggt gtcgctcgcg ggaaaacacc ggctccacgt cgaacagggt ggctttgtgc
     8281 cctttgattc ggacatcacg ctagagcccg gacagacggc ccagatcgcc gcgacgctga
     8341 ccgcgaagga acagccccct cccccaccgc cacctgagtt tgtcaccatc ccaggtgaat
     8401 cgagccgctc gcgcatcgct cgctatgtgc ttgggggcgc tgcatcggga cttggcgttg
     8461 cgctcgtcgg gctcggtggc tgggcacttt cgcaaaacgg ccagtgcggt gacaatcccg
     8521 cgccgcaaaa cggcatcccc tgtgaacttg tgtacaacac cagcaccgtg ggtcgcctgc
     8581 tcatcggcag cggcatcggg ctcacggtgg gtggaagcct gctgatgctg ctgccgggtc
     8641 gccgccccaa acaagtgctc aagtcggcac tgaccccata accaaagcgc acgccgcccc
     8701 acgcctggac gccaaaggtg gggcaaagtt tgggcaccag atgggcaccg aaggggtcta
     8761 gaaacgaaaa aagcccctcc gacatcgtca gaggggctta ttggaggcgc cgggagtcga
     8821 acccgggtcc gaaagtgctt ggcaggggct actac
//
LOCUS       DAORGN010000208         8797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3532_length_8797_cov_5.293182, whole genome shotgun sequence.
ACCESSION   DAORGN010000208 DAORGN010000000
VERSION     DAORGN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_8797_cov_5.293182"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PKL17_17950"
     CDS             complement(<1..881)
                     /locus_tag="PKL17_17950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK46671.1"
                     /translation="MNAVSFGRWVWVCLALHAGCGPRDAVAPPAPSAVADAGTPRAAT
                     ESAPVSSAFAKVQKAEGATLVEVPAQVLGSATQRAEVMPAFRAQVVSVQVEPGQQVTA
                     GTPLVVVRMPEVVRAAGAYLAAGLRQSAFLQRQQQLQSLRGEGLARLSDIAETAASLS
                     EASAAQKEALSTLQAAGLSAQDAAAIADGGGRVTLRSPLAGVVMQVSAATGQIVEPGS
                     SPALVRVAGSGETRIEAHLPILNELPLHFELASAQTTTPLRLLRQAPVLDPRDGMALS
                     WFEPSGPHKLLPGQSGRL"
     gene            complement(878..2140)
                     /locus_tag="PKL17_17955"
     CDS             complement(878..2140)
                     /locus_tag="PKL17_17955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK46672.1"
                     /translation="MRFLVWLGLASLCSLGHASILRAHPASQPLTFAQSIAQAETAPA
                     VKEIEAAQSQRRLGLSTLSAMTHNPQLSVEAGYRDDTGGQGAVVQVAVSQGFHLSGYR
                     AARKQSIEEENAALAHELHSVKFRQKLGVIRLWMALWAQEQVEQLSQEEVALATQIVA
                     QTERLFQAGAATQVDVTTAQTYLAEQKAQQLSAEGEVVEQGLVLGQFIGQKEAVHAAG
                     DPDPVTLPLVQPSLIEEAQRRIATLPEPQHKWSQAQVDKAHAHEAVTQRGSQLWLGAL
                     ALREPTAPYAVFGTLALSIPAFDRGQREQTELLASAARQKVAAQAAEHSALIDLKQTL
                     HDVEHYGQLHNILFAQLLPAAQRQVELRQRLLLAGDGTILEVLMARRSHLQAKAQAAR
                     AKAMLASSQHRLALYLTALGLRNAESAP"
     gene            complement(2199..3602)
                     /locus_tag="PKL17_17960"
     CDS             complement(2199..3602)
                     /locus_tag="PKL17_17960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK46673.1"
                     /translation="MKLATRMWLLGALVPALGVLLGVAVSGRWFRYHLEAAVDRALLA
                     QAAVESVSLFDGPGGRVHLHMATSPLIDSVRLFAPTGDLFGPDGQIVMSYPPRVAVKE
                     QPPEVLLPGVPGQPPRLYTHKRPDGVRCRELTVTVAAPRGGLYTLRLAASLEQLDGSA
                     AAFYRTTLSLAALFGVVLFALQAAAARGLRARLGLLSSHIAALREGQLDQDLPDDPHR
                     DEIGELHRVLQQTTAKLRAARAAQERLLADAAHELRTPLGLMRTSVDLALRRERPKEE
                     LVAAMEEVRSEVVRLGALCQQLLDRFALEQADWAWAQVLLFQVATAAAQSARAEAETR
                     SVLIEVEGSPDVTTSGHEPALRQAVDNLLSNALKFAPPGSSIRISLERSDSVVRLAVQ
                     DEGPGIHPARRAELFAPFRRTPLPHRNPQERAGHGLGLHITREIVARHQGRIFLDETV
                     PQGTRFVIELPSMGSLS"
     gene            complement(3599..4294)
                     /locus_tag="PKL17_17965"
     CDS             complement(3599..4294)
                     /locus_tag="PKL17_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNK46674.1"
                     /translation="MKILIVEDEEKMARLLRRGLQEEGHQADVCASADEAAQQALDIP
                     YDAILLDWMLAEQDGVSLLRSWRQKGLRTPVLMLTARGTTGEKVTGLRAGADDYLTKP
                     FDFDELLARVEALHRRGGNHEPSLQIGSLSLDTRRRVLCGTAGELPLTSREFELLSEL
                     SGHRGDVLTRSKLLSTVWGTDFEGAPNIVDVYIGYLRAKIDQLHGSGVQIQTVRGIGY
                     RLTVDPPRSGERP"
     gene            complement(4778..5515)
                     /locus_tag="PKL17_17970"
     CDS             complement(4778..5515)
                     /locus_tag="PKL17_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNK46675.1"
                     /translation="MARAALVVIGNEILSGKIVDTNTPFLIGELRGLGVALREIVVVP
                     DEVDRIAEALRRVSPRNDHVFTSGGVGPTHDDVTMEGIAAAFSVQVVRHPRLEALLRG
                     YYELQKLPLCEANLRMADVPQGADLLEGPDLRWPVVRMQNIYILPGIPEIFRRKFLAI
                     AETFRDTPFVLRKIYVRSEEGMIADDLSAIAALFPDVAIGSYPRMQPDEDGHRVLLTL
                     EGKEPSRVQSAADQLVSRIASHVLRCI"
     gene            5609..6889
                     /locus_tag="PKL17_17975"
     CDS             5609..6889
                     /locus_tag="PKL17_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46676.1"
                     /translation="MAHRFFALFVLVLRTAGRAAFARAALLYLAIAMVSMIVFAPNAM
                     MASDVTDLAQSSIGFRIVLWSVWLLAATPAARAVLCEPSLFWWRSLPIPRLHFYVAQG
                     LVLALAELPLAILYARGERPLAGIAVLCVNMSLHSLWVSRSRDLPSWIVLFGLPLAVC
                     LPLPLPVLLPLSLLCVVWSLPRAFLAAPHHLLVTHRPAVLGPALLALALTYLVTLWRA
                     HAALFVRACLLLLCGALISFLALRNNQITSASLQNTISLGVLSVTLLLGLSGLAGPVV
                     RCAQHADWLFQVCQRDGRFCLLAEVCALSICGVTLGLLHGILLVLLVGADGARWLPVC
                     ASSAALGLLVAVQTGSFVRLALRGNEKDTDRLLLWLCGLIPLGPVLLWMFHELGLLFL
                     LLLATFMYLRAERQASPKGRWQRLLREREQRGIQ"
     gene            6886..7539
                     /locus_tag="PKL17_17980"
     CDS             6886..7539
                     /locus_tag="PKL17_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK46677.1"
                     /translation="MSLELRNIDKHLGPRKVLSGVSLSVRSDEVVVILGPNGSGKSTL
                     LKLAAGIWEPDAGQITIDGSPLQHGNPAARSRLGYVPDSSDPLPELLVTEFVSLVVRL
                     KQAKPPSPALFETLGVQPYLAQRLSSLSFGQRKRACLLAALIGDPLLLLLDEPSNGLD
                     PGGVAMVVDLIRQRRQQGLATLVATNDTPFAQALSARCLRIANGQLTSVDDPGSVGP"
     gene            7821..8123
                     /locus_tag="PKL17_17985"
     CDS             7821..8123
                     /locus_tag="PKL17_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46678.1"
                     /translation="MKKLVLGLTAVFALATSVPAMADEAPAKPAKAAKKGGKKEGGKK
                     EEGKGEGKKEEGKKEEGKKEEGKKEEHKDEGKHKGEGKGESKKEEHKKEEGKGDKK"
     gene            8273..>8797
                     /locus_tag="PKL17_17990"
     CDS             8273..>8797
                     /locus_tag="PKL17_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNK46679.1"
                     /translation="MPAPSVYFVSLGCPKNRVDTEVMLGMADESGHRITLEPENADVI
                     VVNTCGFIGAAKEESVDTILEMAEFKKTGRCRRLVVTGCLSQRYPDELHREIPEIDHI
                     LGSGEVDKVAQAIRGSEPAVDVAQVPAYLYDHTTPRPASQAHFFRYVKIAEGCDRPCG
                     FCIIPKLRGPQRSRP"
BASE COUNT         1598 a         2708 c         2742 g         1749 t
ORIGIN      
        1 agtcggccac tttgtcccgg caagagcttg tgcggaccac ttggttcaaa ccaggacagc
       61 gccattccgt cgcgcggatc gagcaccggc gcttgtcgca aaagccgcag cggcgtcgtt
      121 gtttgtgcag acgccagctc aaagtggagc ggcagctcat tcaagatcgg caggtgggct
      181 tcgatgcggg tttcgccgct gccggcgacg cgcacaaggg ctggactgct gccgggttcg
      241 acgatttgac ctgtcgcggc gctgacctgc atcaccaccc cggcaagcgg actgcgcagc
      301 gtcacccggc ccccaccatc ggcaatcgcg gcggcatctt gagccgacag accggctgcc
      361 tgtagcgtcg acagcgcttc tttttgggcg gcgctcgctt ccgatagcga tgcggcggtt
      421 tcggcaatgt ccgaaagtcg cgccagaccc tcgccgcgca gcgactggag ctgctgctgc
      481 cgctgcaaaa aggcgctttg ccgcagcccc gccgccaggt acgcacccgc ggcgcgcacc
      541 acttccggca ttcgcacaac cacaagcggg gttcctgccg tgacctgctg tccgggttca
      601 acctgcaccg acacgacttg ggcacgaaac gcgggcatca cttcggcgcg ctgtgtcgcc
      661 gatccgagca cttgcgccgg cacttccaca agggttgcgc cttcggcctt ctgcactttg
      721 gcaaacgcgc tggaaaccgg cgcactttcg gtcgccgcgc gcggtgttcc ggcatcggca
      781 acggcagacg gggccggcgg agcgaccgca tcgcgtggac cacagccagc atgcagcgca
      841 agacagaccc acacccatcg accaaacgaa accgcgttca tggtgcactc tccgcattcc
      901 gaagcccgag ggccgttagg tacaaagcga gtcgatgctg actgctggcc agcatcgctt
      961 tggcgcgcgc ggcttgggcc ttggcttgca gatggcttcg tcgcgccatc agcacttcga
     1021 gaatggttcc gtcgcccgcc agaagcagcc gttgccgcag ctcgacttgt ctttgtgccg
     1081 ccggcaaaag ctgcgcaaaa aggatgttgt ggagctggcc gtagtgttcg acatcgtgca
     1141 gcgtctgttt cagatcgatg agcgcgctgt gttctgccgc ctgcgccgcc actttctgtc
     1201 gcgccgcact ggccaaaagc tcggtctgtt cgcgctgacc gcggtcaaac gccggaatgc
     1261 tcagcgccag cgtgccaaac accgcatacg gggccgtcgg ttcgcgcagt gccaaagcac
     1321 ccagccagag ctgggatccg cgctgcgtca ccgcttcgtg ggcatgcgct ttgtcgacct
     1381 gcgcctgcga ccacttgtgc tgcggctcgg gaagcgtggc gatacgccgc tgcgcttcct
     1441 cgatgagcga cggctggaca agcggcaacg tcaccggatc gggatctcct gccgcatgaa
     1501 ccgcttcttt ttgtccgatg aactgtccca ggacaaggcc ctgctcgacg acttcacctt
     1561 ccgccgaaag ctgctgcgcc ttttgttcgg caagataggt ctgcgccgtg gtgacatcga
     1621 cctgcgttgc cgcgcccgcc tgaaacagcc gctcggtctg ggccacgatc tgtgtcgcca
     1681 gtgcgacttc ttcctgggaa agctgctcga cttgttcttg ggcccacagc gccatccaca
     1741 gccttatcac cccgagcttc tgacggaact tgacggaatg cagctcgtgc gcaagcgccg
     1801 cattttcttc ttcgatgctc tgtttccgcg ccgcgcgata gcccgacagg tggaagccct
     1861 gcgaaaccgc cacttgcacc acggcgcctt gaccgccggt gtcgtcgcga tagccggctt
     1921 caacgctgag ctgcggattg tgggtcatcg cgctgagggt cgaaagcccc agccttcgct
     1981 gactctgcgc cgcttcgatt tctttgaccg ccggggccgt ttccgcctgc gcaatcgact
     2041 gggcaaaggt cagcggctgc gacgctggat gcgcccgcag gatcgacgca tgaccgagag
     2101 agcacaagga agccaaacca agccagacca aaaaccgcat gcccaaacag gtacacgggc
     2161 gctgtggctt tgtgcatgaa tgccaagtaa gaaatttctt aggaaaggct tcccatcgac
     2221 ggaagctcga tcacaaagcg cgttccttgt ggcacggtct cgtccaaaaa aatccgtcct
     2281 tgatggcgcg cgacaatttc gcgggtgatg tgcagtccca gcccgtgtcc ggcgcgctcc
     2341 tggggatttc tgtgcggaag tggcgtgcga cgaaaggggg caaacagctc ggcgcgacgg
     2401 gcgggatgaa tccctggacc ttcatcttgg accgccagac ggaccacgct gtcgctccgc
     2461 tcaagggaaa tccgaatgct gctgcccggc ggggcaaact tgagcgcgtt gcttagcaag
     2521 ttgtcgacag cctgccgcag cgccggttca tgaccgctcg tcgtcacatc gggcgatcct
     2581 tcgacttcga taagaacgct gcgcgtctcg gcttcggccc gcgccgattg tgccgccgcg
     2641 gtggcgacct ggaacagcaa cacctgcgcc cacgcccagt cggcctgttc gagcgcaaat
     2701 cgatcgagaa gctgctgaca cagcgccccg agccgcacca cttcgctgcg cacttcttcc
     2761 atcgccgcga ccagctcctc tttgggtctt tcccgtcgca aggccagatc cacactggtg
     2821 cgcatcagcc caagcggggt tcgcagctca tgtgccgcgt cggccagcag tcgttcttgc
     2881 gccgcgcgtg ccgcccgcag cttggccgtc gtctgctgga gcacccgatg cagctcgcca
     2941 atttcatcgc ggtgcggatc atcgggcaga tcctgatcga gctgtccttc gcgcagcgcc
     3001 gcgatgtggc tgctgagcag tccgagccgg gcccgcagcc cacgcgccgc cgccgcttgc
     3061 agcgcaaaca gcacgacacc aaacagcgcc gccagactta gcgtcgtgcg ataaaacgct
     3121 gccgccgatc cgtcgagctg ttcaagcgat gccgccagcc gcagcgtata aagtcccccg
     3181 cgcggtgccg cgaccgtcac ggttaactcg cgacagcgaa cgccatcggg acgcttgtgg
     3241 gtgtagagcc ggggcggctg tccgggcaca cccggcaaca gaacttctgg cggctgctct
     3301 ttgacagcga cgcgcggcgg atagctcatg acgatttggc catcggggcc aaacagatcg
     3361 cccgtcggcg caaacagccg cacactgtca atcagcggcg atgtcgccat gtgcaagtgg
     3421 acgcgcccgc caggaccatc aaacaggctg acgctttcca ccgcggcttg cgcaagcagc
     3481 gcccgatcga ccgccgcttc caggtgatag cgaaaccagc gaccggacac cgcgacgccg
     3541 agcagcacgc cgagcgcggg caccaatgca cccaaaagcc acatccgggt ggccagcttc
     3601 atgggcgttc tccgctgcgg gggggatcga cggtcagtcg gtagccgatg ccgcgcacgg
     3661 tctggatctg cacgccagag ccgtgaagct gatcgatttt ggcgcgcaga tatccgatgt
     3721 agacatcgac gatgttgggg gcgccctcga agtcggtgcc ccagaccgtg ctaagcagct
     3781 tggaccgcgt aaggacatcg ccgcggtggc cgctcagctc gctcagcagc tcgaactctc
     3841 gactggtcaa aggcagctcg cccgcagttc cgcacagcac ccgcctgcgg gtatcgagcg
     3901 aaagcgatcc gatctgcaag ctgggttcgt ggttgccgcc gcgccggtgc agcgcttcga
     3961 cccgcgcgag cagttcatca aaatcgaacg gcttggtcag atagtcatcg gcccccgcgc
     4021 gcagtccggt gactttttcg ccggtcgtcc cgcgcgcggt cagcatcagc acgggcgtcc
     4081 gcagcccttt ttgccgccag cttcgcagaa gcgacacgcc atcctgttcg gccagcatcc
     4141 agtcgagcaa aatcgcatcg taaggaatgt cgagcgcctg ctgcgcggct tcatctgccg
     4201 aggcacagac atcggcctga tgcccttctt cttgcaggcc gcggcgcaaa aggcgcgcca
     4261 tcttttcttc atcctcgacg atgagaatct tcatggcctg ctatctaacc cgagtcttgg
     4321 gccagaccaa cagaaagtcc catcgcagcg aaaacgtgcg tgttgctgcg gaagcgctgt
     4381 ccacaccgat ttgatcggtc caattggtgc gggaagcgct gccatgcacg ccatgttgcg
     4441 caaaaacagg ctggcaggca ggctacccgc acagtttttg cgcaaaaaga ggttggcagc
     4501 ttggcttccc gcacagtttt tgcgcaaaac gaggttggca gcttggcttc ccgcgcatgt
     4561 tttgcgcaaa acgaggttgg cagcttggct acccgcgcag tttttgcgca aaaagaggtt
     4621 ggcagcttgg cttcccgcac gttttttcgc caaaaccggt gtggaagcgc tgccagcgca
     4681 gtttcgttct gtacattcgt gtgcggaagc gcttcccgca ccgttttgtc tgaccgcttg
     4741 gcttttgtcg cgctgccgca gcgaaagtgg acgtgatcta gatgcagcgg agtacgtgcg
     4801 aagcgatgcg ggacacgagc tggtccgcgg cagactggac ccggcttggt tctttccctt
     4861 cgagcgtgag cagcacccga tggccatctt catcgggctg catgcgcgga taggaaccga
     4921 ttgccacatc cggaaacagc gctgcgatgg cggacagatc gtcggcgatc atcccttctt
     4981 cacttcgcac gtagattttg cgcagcacaa acggggtgtc gcggaatgtt tctgcaatcg
     5041 ccaagaactt gcggcgaaag atttcaggaa tgccgggcaa aatgtagatg ttctgcatcc
     5101 ttacgaccgg ccagcgcagg tccggtcctt ccagcagatc cgcgccctgc ggaacatctg
     5161 ccatccgcaa gttggcttcg caaagcggca gcttttgcag ctcatagtag ccacgcagca
     5221 gcgcttcgag ccgcggatgg cgcacgacct ggaccgaaaa cgccgccgcg attccttcca
     5281 ttgttacgtc atcgtgcgtt ggtcccacgc cgcccgatgt gaacacatga tcatttcgcg
     5341 gactgacgcg gcgcagtgct tcagcaatcc gatcgacttc atcgggaacg acgacaattt
     5401 cgcgcaacgc cacccccaga ccgcgcagct cgccaatcaa aaagggggtg ttggtatcga
     5461 cgatcttgcc agacaggatt tcgttgccga tgacgaccag ggcagcacga gccatgtgcg
     5521 cgagcatatc agcaaaccgg cggcgcttgg gatgatgcgc cgtgcgaaac ttgtttggcc
     5581 gcggacccgc ccgtaggatg cggcggacat ggcgcacagg tttttcgccc tctttgtgtt
     5641 ggtgttgcgg acggcagggc gggctgcgtt tgcgcgcgcg gcgcttttgt atctggcgat
     5701 tgcgatggtg tcgatgatcg tctttgcgcc aaatgcaatg atggccagcg atgtgaccga
     5761 cctggcccag tcatcgattg gttttcgcat cgtgctgtgg tctgtgtggc ttctggcggc
     5821 gactccggcg gcgcgtgcgg tgctgtgtga accgtcgctg ttttggtggc ggagcttgcc
     5881 gattccgcgg cttcactttt atgtcgcgca gggacttgtg ctcgcgcttg ccgagctgcc
     5941 acttgcgatt ctgtatgcgc gcggcgaacg cccgctggct ggcatcgcgg tgctctgtgt
     6001 gaacatgagc ctgcactccc tgtgggtttc ccgatcgcgc gatctgccgt cgtggattgt
     6061 ccttttcgga ctgccgctcg cagtgtgtct gccgcttcct ctgccagttc ttttgccgct
     6121 gtctttgctc tgtgtggttt ggtctttgcc gcgggcgttt ttggcggctc cgcatcacct
     6181 gttggtcacg cacagaccgg cggtcttggg cccggcgctg ctggcgctgg cgcttacgta
     6241 tcttgtgacc ctttggcgag cccatgcggc gctgtttgtg cgcgcgtgct tgcttctgct
     6301 gtgcggcgcc ctgatttcct ttttggcgct gcgcaacaac cagatcacct ctgcatcgct
     6361 gcaaaacacg atatcgctcg gtgtcttgtc agtgacgctg cttttgggtc tgtctgggct
     6421 ggccggtccg gttgtgcgct gtgcacagca tgccgattgg ctgttccagg tctgtcagcg
     6481 cgacggaagg ttttgcctgc tggcggaggt ctgtgcgctt tcgatctgcg gcgtgacgct
     6541 gggactcctt cacgggatct tgctggtgct gcttgtgggg gcagacggtg cgcggtggct
     6601 gccagtctgc gcaagcagcg cggcgctggg tctgcttgtg gcggtgcaga caggaagctt
     6661 tgtgcggctt gcgctgcgtg gaaatgaaaa ggacactgac cggctgctcc tgtggctgtg
     6721 cggactgatt ccgctgggtc cggtgctttt gtggatgttt cacgaactgg gacttttgtt
     6781 tctgctgctt ttggcgacgt ttatgtattt gcgcgccgaa aggcaggcgt cccccaaagg
     6841 acgctggcag cggcttctgc gtgaacgaga acagcgaggg atacagtgag ccttgagcta
     6901 cgcaacatcg acaaacacct gggcccccgc aaggttctgt ccggagtatc gctttcggtt
     6961 cgttccgatg aagtggtggt gattttgggg ccaaacggaa gcggcaaatc cacgctgctc
     7021 aagctggcgg caggaatctg ggaacccgat gccggacaga tcacgatcga cggcagtccg
     7081 ctgcaacatg ggaatccggc ggcgcgcagc cggttgggct acgttcccga tagctccgat
     7141 ccacttccgg aactgttggt gacagagttt gtgtcgcttg tggtcaggct caagcaggcg
     7201 aagccaccgt cccccgcgct tttcgagacg ctgggcgtgc agccgtattt ggcgcagcgg
     7261 ctgtcttccc tgtcgtttgg acagcgcaag cgggcgtgtc ttttggcggc gctcattggc
     7321 gatcctttgc tgctcttgct ggacgagccg agcaacggcc ttgatcccgg tggcgtcgcg
     7381 atggtggtgg atcttatccg acagaggcgt cagcaggggc tggcgacgct tgtggcgacc
     7441 aatgacacgc catttgcgca ggcactgtcc gcacgctgtc tgcgcatcgc aaacggacag
     7501 ctgacttcgg tcgacgaccc aggcagcgtg ggtccgtaag gcgcgctaag acggtgggtt
     7561 gggcggctcc attcattgaa aaatgggtcg cgtttccgtc ggacaaaata gccacacaat
     7621 tggcgccaag ccgccccacc caagccgcaa atgcggtcgc atttgggggc ggtttgtgca
     7681 accagcgagg ccgctcagat tcccattgtt tggcacaggg aatgcaatcc cttgtttgcg
     7741 aggcgccaca ccacgcggcg cagagcggtc acatcacagt ggctgactca caaacaacct
     7801 ttttgtcctg gagtgtcatc atgaaaaagc tcgtgctggg tctgactgct gtgttcgcgc
     7861 ttgccacctc ggttccggcg atggctgacg aagcccccgc caagcctgcc aaggccgcca
     7921 agaagggcgg aaagaaggaa ggcggcaaaa aggaagaggg caagggcgaa ggcaagaagg
     7981 aagagggcaa gaaggaagaa ggcaagaagg aagagggcaa gaaggaagag cacaaggacg
     8041 aaggcaagca caagggcgaa ggcaagggcg aaagcaagaa ggaagagcac aagaaggaag
     8101 agggcaaggg cgacaagaag tagtcgaacc tggcttcggc ctgcttttcc aagcaagaga
     8161 gccttggcga cggcatctaa gccggccagg gctctttgca ttttggcgcc acaaatcaga
     8221 caaattccga ggacggcttg acagcgccgg gcaagctgag catgctgccg ccgtgcctgc
     8281 tccttcggtt tattttgtca gcctcggatg tcccaaaaat cgcgtcgaca cagaagtcat
     8341 gcttggcatg gcggatgaat cgggccaccg gatcacgctc gagcccgaaa atgccgacgt
     8401 gattgtcgtc aatacctgtg gtttcatcgg tgccgccaaa gaggaatcgg tcgataccat
     8461 cctcgaaatg gcggagttca aaaagaccgg tcgctgtcgt cgcttggtgg tgacgggctg
     8521 tctttcgcag cgctatccgg acgagctgca ccgcgagatt ccggaaattg atcacatcct
     8581 gggttcgggg gaagtcgaca aagtcgcgca agcgataagg ggcagcgagc cggcggtcga
     8641 tgtcgcccaa gttccggcgt atctttacga tcacacaacg ccgcggcccg cgtcgcaggc
     8701 gcactttttt cggtacgtga aaatcgccga aggctgcgac cgtccgtgcg gtttctgcat
     8761 cattccgaaa ctgcgcggcc cgcagcgcag ccgcccc
//
LOCUS       DAORGN010000209         8770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3550_length_8770_cov_5.486976, whole genome shotgun sequence.
ACCESSION   DAORGN010000209 DAORGN010000000
VERSION     DAORGN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_8770_cov_5.486976"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2432
                     /gene="gyrA"
                     /locus_tag="PKL17_17995"
     CDS             <1..2432
                     /gene="gyrA"
                     /locus_tag="PKL17_17995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNK46680.1"
                     /translation="DASVYDALVRLAQDWNMRYPLVDGQGNFGSIEGDPPAAYRYTEC
                     RLQRVAASMLADLDKETVDFVPNFDDKEREPTVLPALLPNLLVNGAAGIAVGMATNIP
                     PHNMGEVLDAALHLLKEPTATNADLMRLVPGPDFPTWGFIYGRAGIRQAYETGRGSLT
                     LRARTEIEELKNGKSQIIVTEVPYQVNPNRVIERLAELVREKKIEGISDLRNESSREG
                     MRLVIELKRDAVSQVVLNQLFQQTSLQVTFGVMMLAIVDGRPRILSLRDAIGQYLEHR
                     RQVVLRRSLYMLKQARARREIVEGLGLAIDQIDRIIAIIRAAKDPAEAKEHLMAEPLT
                     GLAEFLRRAGRPEDEIAARTKGGVLYFSEPQAKAILDMRLQRLTGLERDKLAEEYRDL
                     TATIVDLEGILASEVRLREVIAGELSELRGQYTEPRRTEIIDDAGEIRLEELIAEEPM
                     VVTLSREGYVRRVSLSEYRAQGRGGRGVQGASTKEEDLIHKLFVASTHDYVLMFTNTG
                     RAYSKRVFDLPESQSRAGKGKAMVNFLELQPGERIVEMLPLPSFDAGKFVMLATAKGV
                     VKKTELDAFSAIRSTGIIAVDLDEGDDLVGAAITDGQSDIALCTRDGYVVRFREEQVR
                     AMGRTARGVRGVTLKSSTDRVVAMQVLSRDLGSDITLLTVCEKGFGKRTPASDYPQKN
                     RGTQGVITIKTNDRNGKVVGVLTVVDSEDLMLVTDTGRVIRIRVRDIPTRGRNTQGVR
                     LIRIDPASGERVVAVEALSDPGKDGSEPGEAEGEELAASPIEVLDDGEGEEEITDEAE
                     ADGGSDEPEAS"
     gene            2432..3256
                     /locus_tag="PKL17_18000"
     CDS             2432..3256
                     /locus_tag="PKL17_18000"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNK46681.1"
                     /translation="MSEKTTPPEPVQTGPLSVVEIYRRSVKAEDLRFNRYIARPKAAL
                     LVYLLRPTPITPNQVTLLSLVVSLVGMATLLLCPGLTGLVAAALVLHLAFVLDCVDGQ
                     LARIKGQSSPVGAYLDFLVDEIKAVLLIACCAGRLAIESRAPLCGIPLAPQVFWLAVG
                     LVGVCIAASGISTTTFMRRPEYFEAVHGKKGERVAGYTKFKEPEAAKPTSLVGTLVRL
                     PIRLLEAFGKITLHYPAWFFLPAIFGKIEWFLLPYLAAHTMYLGRSWLTVIWKLGR"
     gene            3253..4305
                     /locus_tag="PKL17_18005"
     CDS             3253..4305
                     /locus_tag="PKL17_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46682.1"
                     /translation="MITRREAVQTLALSAAGGLLFPSTVSAKTAMPLQRGVALGLFHE
                     DPLWSYLGLLREIKELSATHVSLVPAYYQDHAASTSIYSHPRFSVPDETIVRTVGEAH
                     ALGLKVMLFPIVRLAAPRSDSEWRGTLQPDDRKAWWNSYERLILHLAKLCKAQNVAQF
                     SIGSELSTLDGERDLPQWQSLVEKVRATCKSGLTYSGNWDHFDKVAIYGLCDTAGMCA
                     YFPLASRLSIPPIPVEDMVSAWQKKRDELVTFAKKRNRPLLLTEVGYLSQRGACAWPW
                     EEGAEKPVDLEDQRRAYEAFAQVWQGEPALSGVYFWNYYGFGGRASRGYTMRHKPAAQ
                     EMERYFLSESARPALP"
     gene            complement(4339..6183)
                     /locus_tag="PKL17_18010"
     CDS             complement(4339..6183)
                     /locus_tag="PKL17_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46683.1"
                     /translation="MGFLLWAGCSSGGAPPFDESSAVDLSQQSPGRDGGTVDMGMNPG
                     SLCEPPCWLNPLPQGNYLEDVYRDAKGNGWAVGGNSLIRIEGDRTTLVRHPVADKQNF
                     LSSVWGCGENEVWVAGTAVLHYDGKTWTDLPEFSWKGIEDVFCTGPSDVWLVGISGQI
                     HHYDGKTWQKSTSGITTALRQISGSSPSNIWAGGDYGVLLFYNGSTWQKQTSPVTDTI
                     GSLSCSDRGDCWMIPFPGVMYPGISGSDVYRLEGGRFVKQTVPASMIYYGVSAVSASE
                     VWLAGTFDYLLSSGSWQKTTPQVYSLRSIAGQGLPSGRDQIGVGAAGLIGRFDGTAWR
                     TLSSGRTEELRGTYSSPQGEQYFVGRSGAVGRIKSGQIEWQSLQGGSSSYYALWGISG
                     SDIWAVGEYGHLAHFDGRDWTQLPERFGGSLEKLLAVSGSAASDVWAVGDQGTIVHYD
                     GARWSTVTSGLAIKSLAGVYAASANDVWIGGADGKLGHFDGSKWTQWPKTWSKDIVAM
                     WGASASDIWIAGGWTTTKDFFLARWNGSDWIAQTTPAGLTYPASISGTGPKDVWVGGH
                     YGELVHFDGTSWAQFPLATNFTIFGVGQSQKTHYAIGDTGLIIQLDRR"
     gene            6528..7808
                     /locus_tag="PKL17_18015"
     CDS             6528..7808
                     /locus_tag="PKL17_18015"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNK46684.1"
                     /translation="MSTQSGPTETNTQPGSSRPLVEGRQALWLGLLAALVAGAVLCGY
                     EMLRSSANTLFVQAYGKQALPWALGLTLVGVTLLTFIYGRLLSWLGPRHTLQATTALA
                     ILTMVASWGAIRLGLRPALIVLFIFKESYVVLLIEQIWSYIDSTLEPKAAKRLNGPIC
                     GVAGIGAVAGGLLLGKLSQPLGTQSMLLLAAAATLLTLPLLQLLFAKFGAPTEGKKKQ
                     SEKGHLALHLFRTEPVLLLLISLIASTQVLSTILELAFKSALQDHLPSADVQNAYSGN
                     YYALVNFAAMFFQFIGAPLLLSVFPGRVIHILVPLCNLLALGYMLWAPPLIGSAVAYG
                     VFKTLDYSLFRAAKELLYVPLPFDARYRAKEVIDVLGNRVSKGMASLLVTLLQFAHVV
                     FSQLAYAGVGIVAVGLWLGLALPLSRAAQKEQAE"
     gene            7805..>8770
                     /locus_tag="PKL17_18020"
     CDS             7805..>8770
                     /locus_tag="PKL17_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNK46685.1"
                     /translation="MTGNPAKAPILPNGPRSSLSTYLWRYAPRHIVGGVMLLGFQVAM
                     NRIDWLSKQAIDTIFGRIPSRAPSDAMWPAMMMLGLAVFAFIVRVASRMYCFNAGRDI
                     EYELRGQLLSRLHRLGFSFYRRLSAGEIMSRATSDLTQVRLLTGFGVMNLINIVFALV
                     SALQVMLSISPRLTLVSFTTVPLLMLLTRTFSGKMFSRTQKVQETLGKLTEFVQSNLA
                     GIRVVRSFGIESNIEQRFEELNQRYLSTSLDLARLRGLMFPVTGAVAALGLLLFFWYG
                     STLLLNGSLTEGGFFAFSLALARMTWPLIAAGFLMSIVQRGRVSFN"
BASE COUNT         1689 a         2627 c         2666 g         1788 t
ORIGIN      
        1 gtgacgcatc ggtctacgat gcactcgttc gactggcgca agactggaac atgcggtatc
       61 cgctcgtcga cgggcaagga aacttcggat cgattgaagg cgatcccccg gcagcgtatc
      121 ggtacaccga gtgtcgtctg cagcgggttg cggcgtcgat gctggccgat ctcgacaaag
      181 agacggtcga ttttgtcccg aacttcgacg acaaagagcg cgagccgacc gttctgccag
      241 cgcttttgcc aaacctcttg gtcaatgggg ccgccggtat cgccgttggt atggcaacca
      301 acattccgcc gcacaacatg ggcgaagtgc tggatgcggc gctgcatctt ctaaaagaac
      361 cgaccgcgac caacgccgac ctgatgcggc tggttccggg tccggacttt ccgacgtggg
      421 gctttatcta cggacgcgcc gggattcgcc aagcgtatga aaccggacgc ggatcgctga
      481 cgctgcgcgc ccgcaccgaa atcgaagaac tgaaaaacgg caagtcgcag atcatcgtca
      541 ccgaggttcc ctatcaggtg aacccgaacc gcgtgatcga acggctcgcc gagctcgtgc
      601 gcgaaaagaa gatcgagggc atttccgatc tgcgcaacga atcgagccgc gaaggcatgc
      661 gccttgtgat cgagctcaag cgcgatgcgg tgtcgcaggt cgtgctcaat cagctgtttc
      721 agcagaccag cttgcaagtg acgttcggcg tcatgatgct ggcgattgtc gacgggcgtc
      781 cgcggattct gtcgctgcgc gatgcgattg ggcagtacct cgaacaccgt cggcaagtcg
      841 tgctgcggcg atcgctttac atgctcaaac aggcgcgggc acgacgggaa attgtcgaag
      901 gcttgggcct ggcgattgat cagatcgatc ggatcatcgc gatcattcgc gctgccaaag
      961 atcccgccga agccaaagag cacttgatgg cggaaccgct gaccggactg gctgaattcc
     1021 tccggcgagc gggtcgcccc gaagacgaga tcgcggcgcg gaccaaaggc ggcgtgctgt
     1081 actttagcga accgcaggcc aaagcgatcc tcgacatgcg gctccagcgg ctgacgggac
     1141 tggaacgtga caagctggcc gaagagtatc gggatctgac cgcaaccatc gtcgatctcg
     1201 aaggcatcct cgccagcgaa gtgcggctgc gcgaagtcat tgccggcgag ctgtccgagc
     1261 tgcgcggtca gtacaccgaa ccgcgccgca ccgaaatcat cgacgacgcg ggcgaaatcc
     1321 gactcgaaga gctgatcgcc gaagaaccga tggtcgtcac cctgtcgcgc gaaggctatg
     1381 tccgtcgcgt gtctttgtcg gaataccggg cgcagggccg aggcggtcgc ggtgtccagg
     1441 gcgcttcgac caaagaagag gatctgatcc acaagctgtt cgtcgccagc acccacgact
     1501 acgtgctgat gttcaccaac accggacgcg cgtactcgaa gcgcgtgttc gatttgccgg
     1561 aatcgcagtc gcgcgccggc aaaggcaaag cgatggtgaa ctttttggag cttcagcccg
     1621 gcgaacggat cgtcgaaatg ctgccgctgc cgagctttga cgccggcaag tttgtcatgc
     1681 tggcgacggc aaaaggcgtc gtcaaaaaga ccgagctgga tgctttttcc gcaattcgat
     1741 cgaccggcat catcgcggtc gatctcgatg aaggcgatga tctggtgggc gccgccatca
     1801 ccgacgggca aagcgacatt gcgctgtgta cccgcgacgg ttatgtggtg cgattccgcg
     1861 aagagcaagt ccgcgccatg ggccgcaccg cacgcggtgt tcgcggcgtc acgctcaaga
     1921 gcagcaccga ccgcgtcgtc gcgatgcagg tgttaagccg cgatctgggc agtgacatca
     1981 cgctgctcac cgtctgcgaa aagggctttg gcaagcgcac cccggcttct gactatccgc
     2041 agaaaaaccg cggcacccag ggtgtcatca ccatcaagac caacgatcgc aacggcaaag
     2101 ttgtcggtgt gctcaccgtg gtcgacagcg aagatttgat gctggtcacc gacacgggtc
     2161 gcgtcatccg cattcgcgtg cgcgacatcc caacccgcgg tcgcaacacc caaggcgtgc
     2221 ggcttatccg catcgatccc gcaagtggcg agcgcgtggt cgccgtcgaa gccctgagcg
     2281 atccgggcaa agacggcagc gagcccggtg aagccgaagg cgaagaactg gccgcgagcc
     2341 cgattgaagt gctggacgat ggcgaaggcg aagaagaaat caccgatgag gccgaagcgg
     2401 acggcggcag cgacgaaccc gaggcgagct gatgagtgaa aagaccaccc cgcccgaacc
     2461 ggtgcagacc ggtccgcttt cggtggtcga gatctatcgg cgcagtgtga aagccgaaga
     2521 cctccggttt aaccgctaca tcgcccggcc caaagcagcg ctgctggtct atctgctgcg
     2581 tccgacgccg atcacgccca atcaagtgac gctgttgtca cttgtcgtgt cacttgtggg
     2641 catggcgacg ctgcttttgt gtccgggcct tactggactt gtggcggcgg cgctcgtgct
     2701 gcacctggcg tttgtgctcg actgcgtaga cggacagctt gcacggatca aaggccagtc
     2761 ttcgcccgtc ggggcgtatc tcgatttttt ggtcgatgaa atcaaagcgg tgctgctgat
     2821 tgcctgctgt gccgggcggc tggcgattga atcccgtgcc ccgctgtgtg ggattccgct
     2881 tgcgccgcag gtgttctggc tggccgttgg acttgtcggc gtctgcatcg cggcgtcggg
     2941 gatttcgacc acgacgttta tgcgcaggcc ggaatacttt gaagcggtcc acggcaaaaa
     3001 aggcgagcgc gtcgccggct acaccaagtt caaagaaccg gaagcggcca aaccaacgtc
     3061 gcttgtggga accctggtcc gtctgccgat tcgacttctg gaagcgtttg gcaagatcac
     3121 cctgcactat ccggcgtggt tttttctgcc tgcgatcttc ggcaaaatcg agtggtttct
     3181 gctcccctat ctggctgcgc acacgatgta tttgggtcgt tcgtggctca cggtgatctg
     3241 gaagctcggg cgatgataac gcgcagggaa gcggtccaga cacttgcgct ttcggcggca
     3301 ggaggactcc ttttcccgtc gacagtttct gcaaagaccg cgatgccgct tcagcgcggt
     3361 gtggcgctcg gtctgtttca cgaagatccg ctgtggtcgt atttgggcct gctgcgcgaa
     3421 atcaaagagc tgtccgcgac ccatgtctct ttggtgccgg cctactacca agatcacgcc
     3481 gcatcgacca gcatctacag ccacccgcgc ttcagtgttc ctgacgaaac catcgtgcgc
     3541 acggtgggtg aagcgcacgc gctgggactc aaggtgatgc tgtttccgat tgtccggctg
     3601 gcggcgccgc gcagtgactc cgagtggcgc ggaacgctac agcccgacga tcgcaaggcc
     3661 tggtggaaca gctacgagcg gctgattctg catcttgcca agctgtgcaa agcgcaaaac
     3721 gtcgcgcagt tttcgatcgg cagtgagctg tcgacgctcg atggcgagcg cgatcttccg
     3781 cagtggcaga gcctggtcga aaaagtgcgc gcgacgtgca agtccggcct gacctattcc
     3841 ggaaactggg atcacttcga caaagtcgcg atctacgggc tttgcgatac cgcagggatg
     3901 tgcgcgtatt ttccgctggc gagtcgcctg tcgattccgc ccattcccgt cgaagacatg
     3961 gtgtcggcgt ggcaaaaaaa gcgcgatgag ctggtcacgt ttgccaaaaa aagaaatcgt
     4021 ccgctgctgc tcacggaagt gggctaccta agccagcgtg gcgcgtgcgc gtggccgtgg
     4081 gaagaaggcg cagaaaagcc ggtcgatctg gaagatcagc gtcgagcgta tgaagccttt
     4141 gcacaggtct ggcaaggcga accagcgctg tccggtgtct acttttggaa ctactacggc
     4201 tttggtgggc gcgcgtcccg cggctacacg atgcgccaca aacccgcggc ccaggaaatg
     4261 gaacggtatt tcctttccga gtccgcgcgg cccgcgctgc cgtaactttt ttgcagcctg
     4321 tggtccgcgc ggccagacct atctgcgatc aagctggatg atgagtccgg tgtccccgat
     4381 cgcgtagtgt gtcttttggc tctgtccaac gccaaagatg gtgaagtttg ttgccagcgg
     4441 aaactgtgcc cagcttgtgc catcgaaatg aaccagctcg ccgtagtggc ccccgaccca
     4501 gacatccttc ggtcccgttc ccgatatcga ggccggatag gtcaggcccg caggcgtagt
     4561 ctgggcaatc cagtcactgc cgttccagcg cgccaggaaa aaatctttgg tcgtggtcca
     4621 gccgcccgca atccaaatgt cagaagcgga agcgccccac atcgcaacga tgtctttgga
     4681 ccaagtcttt ggccactgcg tccacttgct gccatcaaag tgaccgagct tgccatcggc
     4741 gccacctatc cacacgtcgt ttgcagaagc ggcatagacc ccggccagcg acttgatcgc
     4801 cagaccgctt gtgaccgttg accagcgcgc gccgtcataa tgcacgatgg ttccttgatc
     4861 gccgaccgcc cagacatcag acgcggcact gcccgacacc gcgagcaatt tttccagcga
     4921 accgccaaac cgttccggaa gctgggtcca gtctctgcca tcaaagtgcg caagatgtcc
     4981 atattcaccg acggcccaga tgtcgctgcc gcttatgccc cacagggcgt agtagcttga
     5041 cgaaccgcct tggaggctct gccactcgat ctgtccggac ttgatccgcc cgacggcacc
     5101 cgaccttccc acgaaatact gctcgccttg cggactgctg tacgtgccgc gcagctcttc
     5161 ggtacgaccg ctcgacaacg ttcgccatgc cgtgccatca aagcgcccga taagaccggc
     5221 tgcgccaacc ccaatctgat cacgtcccga cggcagacct tgcccggcaa tgctgcgcag
     5281 cgaatagact tgcggcgtgg ttttttgcca gcttccactg ctgagcaggt aatcaaacgt
     5341 ccccgcgagc cagacttccg acgcagatac cgcgctgacg ccgtaataga tcatgctcgc
     5401 tgggacagtc tgtttgacaa agcgcccgcc ttccaagcga tacacatcgc tgccggaaat
     5461 tccggggtac atgacgccag gaaacggaat catccagcag tcgccgcgat cggaacagga
     5521 cagcgatccg atcgtatctg tgacagggct tgtctgcttt tgccacgtcg agccgttgta
     5581 gaaaagcaga acgccatagt cgccaccggc ccaaatattc gacgggctgc tgccgctgat
     5641 ttgtcgcagc gcggtggtga tcccacttgt ggatttctgc cacgtcttgc cgtcgtagtg
     5701 atggatctgg ccgctgattc ccaccaacca gacatcgctt gggccagtgc aaaagacatc
     5761 ttcgatgcct ttccacgaaa actcgggcag gtcggtccag gtcttgccgt cgtaatgcaa
     5821 gaccgcggtg cccgcgaccc agacttcgtt ttcaccacaa ccccaaacgg acgacaggaa
     5881 gttctgtttg tctgccaccg gatgacgaac cagcgtggtg cggtcgccct cgatgcgaat
     5941 cagcgagttg ccaccgacgg cccagccatt tcccttggca tcgcgataga catcttccag
     6001 gtaatttcct tgcggcagcg gattcaacca acagggcggt tcacaaagtg aacccggatt
     6061 cattcccatg tcgaccgtcc cgccatcgcg accgggactt tgctgcgaca aatcgacagc
     6121 ggacgattca tcaaaaggcg gcgccccgcc gctgctgcag ccagcccaca gcaaaaaccc
     6181 cacagcagac acccgagcac aagaagcgaa ggtcaagcga gacataaacc gtccttttcc
     6241 atacgttttg cttgggtaaa acgattctcg acgggtgtca accgatcttg cgacgctttg
     6301 cgggcagagc acggcccggt aaaaaagcga gcgcgggcga acgcgcaacc ggcctgcgga
     6361 ccgattccgc ggtctgccaa cacacgccgc ttggtgacgg aatcactcga cttcccgccg
     6421 ccgcgcatgc gctgtcccgc gaatccgttt gtcctcgggg acggttttcg ctagggtgat
     6481 cggactgtcg ctcgcaaatg gggcggcagc ttggcggaac agcgcggatg tcgacccaga
     6541 gcggacctac ggaaacaaac acgcagcctg gatcgtcgcg cccgctggtc gaaggacggc
     6601 aggccttgtg gcttgggctc ttggcggcgc tcgtcgctgg cgcggtgctg tgtggttatg
     6661 aaatgctgcg ctcgtctgcc aatacgctgt ttgtgcaggc gtatggaaaa caagcgctgc
     6721 cgtgggcgct gggtctgacg ctcgtcggag tgaccctgtt gacgttcatt tacgggcggc
     6781 ttttgtcgtg gctgggaccg cggcacacgc tgcaagccac gacggcgctg gcgattttga
     6841 cgatggtcgc aagctggggc gcgattcggc tgggacttag gccggcgctc atcgtgctgt
     6901 tcatcttcaa agagtcttac gtcgtgctgc tcatcgagca gatttggtcg tatatcgaca
     6961 gcacgctcga accgaaggcc gccaaacggc tcaacggtcc gatctgcggc gtggcgggaa
     7021 ttggtgccgt cgcgggcggg ctgcttttgg gcaagctgtc gcagccactg ggaacgcaaa
     7081 gcatgctgct cttggcggcg gcagcgacgc ttttgacgtt gccgcttttg cagcttttgt
     7141 ttgcaaagtt tggcgcgccg acggaaggaa agaaaaaaca gtcggaaaaa ggccacctgg
     7201 cactgcatct gttccgaacc gaacccgtgc tgttgctgct gatttcgctg atcgcttcga
     7261 cgcaggtgct atcgacgatt ctggaactgg cattcaagag cgcgcttcaa gaccacttgc
     7321 cgagtgccga tgtccaaaat gcgtactcgg ggaactacta cgcgctggtc aactttgccg
     7381 cgatgttttt ccagttcatc ggtgcgccgc tgttgctatc ggtgtttccg gggcgtgtga
     7441 ttcacattct ggtgccgctg tgcaatctgc tggcgctcgg ttacatgctg tgggcgccgc
     7501 cgctcatcgg cagtgccgtt gcttacggcg tattcaagac gctcgattac tccctgtttc
     7561 gcgccgccaa agagctgctg tatgtccctt tgccatttga cgcacgttat cgagccaagg
     7621 aagtcatcga cgtgctcggc aaccgcgtca gcaaaggcat ggcttcgctt ctggtgacgc
     7681 tgcttcagtt tgcccatgta gtgttttctc agcttgccta tgcgggcgtt ggcatcgtcg
     7741 ctgtcgggct ctggctcggt ctggcgttgc cgctttcccg cgcggcgcag aaagaacaag
     7801 cggaatgact ggaaaccctg cgaaagcgcc gatcttgcca aacggtccgc gctcgtcttt
     7861 gtccacgtat ctgtggcgct atgccccccg tcacatcgtt ggcggcgtga tgctgctcgg
     7921 ctttcaggtg gcgatgaacc gcatcgactg gctgtcgaag caagcgatcg acaccatctt
     7981 tggtcggatt cccagccgcg cgccgagcga tgcgatgtgg ccggcgatga tgatgctcgg
     8041 tctggcggtg tttgcgttta tcgtccgcgt cgccagccgg atgtactgct ttaacgccgg
     8101 acgcgacatc gaatacgagc tgcggggcca gcttctttcg cgcctgcatc ggctcggttt
     8161 ttcgttttac cgtcggctgt ccgcgggcga aatcatgagc cgggcgactt cggatctgac
     8221 gcaagtgcgg cttctcaccg gctttggcgt catgaacctg atcaacatcg tgttcgccct
     8281 ggtgagcgcg ctccaggtca tgctgtcgat ctcgccgcgg ctgacgctgg tgtcgtttac
     8341 gacggtgccg cttctgatgc tcttgacccg gacattttcg ggaaagatgt tttcgcgcac
     8401 gcagaaagtg caggaaacgc tcggcaaatt gaccgagttt gtccaaagca acctcgccgg
     8461 cattcgggtg gtgcgcagct ttgggattga atccaacatc gagcagcgct tcgaggaact
     8521 caaccagcgc tatctgtcga cgtcgctcga tctggcgcgg ctgcgcggcc tgatgttccc
     8581 ggtgacgggt gcggtggcgg cgctcgggct gctgttgttt ttctggtacg gatcgacgct
     8641 gcttctaaac ggcagcctga ccgaaggcgg ctttttcgcg ttttccctcg cactggcgcg
     8701 gatgacgtgg ccgctgattg ccgccgggtt cctgatgtcc atcgtccagc gcggtcgcgt
     8761 aagctttaac
//
LOCUS       DAORGN010000210         8750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3563_length_8750_cov_4.850144, whole genome shotgun sequence.
ACCESSION   DAORGN010000210 DAORGN010000000
VERSION     DAORGN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_8750_cov_4.850144"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKL17_18025"
     CDS             complement(<1..601)
                     /locus_tag="PKL17_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46686.1"
                     /translation="MQIPTLSSQMSPSALRRELRYTLVRLRTPAWATTYRTTFESLLT
                     LSESAIMEHIKLRDAAEDADAQLDQLDSELDEFALYLFKFMMAETTGTGRATLRQALF
                     GTEKPSRFVRPRLGDKLARMRNWPTVLNGAPLAKLVAVGKDVDALIKRCDSALSAHAT
                     ATANLQAFEFNTWTPFVAKVNGERQLLGGEAKKQYRLDGS"
     gene            861..1115
                     /locus_tag="PKL17_18030"
     CDS             861..1115
                     /locus_tag="PKL17_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46687.1"
                     /translation="MSWGSFGRPAGRTTIGRPDSGPDGRTLLDLQKLLGPQSGQVRTT
                     PRSQGRLAATCTKGKTGAGSHGPSGDGKVDQVTEDGPRRD"
     gene            complement(1112..2392)
                     /gene="rlmN"
                     /locus_tag="PKL17_18035"
     CDS             complement(1112..2392)
                     /gene="rlmN"
                     /locus_tag="PKL17_18035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNK46688.1"
                     /translation="MSTLTPLSTSAPSPADPTTHSPSLQGTPIELRAQTYDDLTAFMQ
                     SLGEKRYRGEQLFRWIHARGVTTLDAMTDVSAKLRARLQAENLVQVSELDIDKVQIAA
                     DGTRKLRLRTADNRLIESVLIPDDDARQIIAGQLQQSRTLDAYDDEEEAPTWERKKMT
                     QCVSSQVGCAIDCDFCATAKLGFGRNLSAGEIVTQIYQAESLLRSLPADDPTRLGGGD
                     VVTNLVFMGMGEPLHNYDNLVRALRILLHPLGRSFSRRRVTVSTSGLVPQIEKLAREG
                     LGVNLAVSLNATTDEVRDVVMPINRKYPLSVLLSALRRFPLDRRQRITVEYVLLGGVN
                     DSIEDARRLPELLRGIPTKLNLIPWNPHPHSSYRRPEQKTVAAFQAECMRLGFTTYVR
                     KTRGDDIDAACGQLAAENDPRAGRQRPGSLPVLS"
     gene            complement(2462..3460)
                     /locus_tag="PKL17_18040"
     CDS             complement(2462..3460)
                     /locus_tag="PKL17_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46689.1"
                     /translation="MSLASSAQSAFRWSGSLKETSIRQLYERCQRLRLTGNMRLQQGD
                     QTLDLMWIGGEPIENEGDQGTRSLPLWTNGEFIVEQRIPDWKGRLTPGIELTGSLRSG
                     QVTAIYKLCADNQLSADVELLRTSGDVAQVRFTLGKAESAVIAGQPESAIAAMSKLGG
                     WVEGTFRVVLRPAFGDEKAAEAPVFKGQPGDKFDVTGNVDLSKDQINWPTAAGASGTS
                     NPPRPASASASSQRNATSNPPKAAPSSTSGGGAAGNSSSAIPPRLAQTLIKAPAPAGL
                     APPSTAATVPMSTATKEQIAAAQKSASGSSNKMVVIISVVVITLCLGGIAAALFLR"
     gene            3598..4734
                     /locus_tag="PKL17_18045"
     CDS             3598..4734
                     /locus_tag="PKL17_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46690.1"
                     /translation="MNDGFRARLGAKKIVSTLSEVSLMRRFTHVQGMFLGAACGFLLS
                     AAGAGCGGDDGTMDPNNMPPAPVCDAKAVTGTDNKAATDALKLPGVSGNKTYVYDFDG
                     DNRQENQLKNLINTISLSGLDIQASVNKAVQSGDAIVLMNLKTGDIMKADCASVTMGL
                     AKGPAPMAPAPKFDGSDTFQMLPDITPVSLFGKIDGGKLSTLASKEQRSDKQTEQQIQ
                     VNLPLGDGKNLPLALHGAHLEGTVVKDGSVIRIKDGVLHGVLAQKDIDDKIVPLVANL
                     LTDMINKDPMSDTAKTIIGLFENQNDAVTKKKCMDNAKDCCKTNPTTCKILPAEVKNS
                     AVGGVLSSDVQVFDDNGNWAPVAGGKKYNGMSVGIGFTGVSAKF"
     gene            4863..5960
                     /locus_tag="PKL17_18050"
     CDS             4863..5960
                     /locus_tag="PKL17_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNK46691.1"
                     /translation="MNDAHSYVVILAGGGGTRLWPHSRRSRPKQFLPMLPGGETLIGA
                     TARRTSDVVPIDRTLVVTTAEQVPELRRCLPDLPIDNILVEPTGRNTAPCIGLAAMVL
                     WKRDPQAVMAVLPSDHYVANEAGFRATLTEALAVAHAGHVVTLGILPTHPETGYGYIE
                     LGPELDGQRVAAAFVEKPNRQRAEEYLSSGRYLWNSGTFFFPAKRILDELRTQLPALA
                     DILDELGNHPERTAVRYPEAPAISIDYAVMERLGQNQAGDARAIRVLAGDFGWNDVGS
                     FAALDALHPADDKGNHVFASSATTPRPVLVDAARNLVWNDGKRLLALIGVEDLIVATT
                     DDAVLVMPRQRAQQVKDAVTLLQKEGRTEFL"
     gene            5957..7999
                     /locus_tag="PKL17_18055"
     CDS             5957..7999
                     /locus_tag="PKL17_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNK46692.1"
                     /translation="MNPLMFREYDIRGIADRDLPDDVVFDLGQGIGTLLFDSGGQRIA
                     LGRDCRLHSPRLHAALTAGLVASGADVVDLGVVPSPMLYFGAHTLDVHGGVQITGSHN
                     PPSDNGLKILLHKRSQGGEQIQKLRQLVEAKAFRKGQGSVRTLALESEYLTFLQQRLA
                     IGARRFPVVLDAGNGTGGSVALPLFQSLGFPTLGLGCEPDGRFPLHPPDPTVPENLRL
                     LQDTVRRTGAEVGFALDGDADRLVAVDRQGRILWGDQLLILLSRAILAEQPGASFVCE
                     VKCSRTVVDEIRRAGGHPIMWQVGHTRIKEKMEESGAALGAEMSGHLFFAHRYLGFDD
                     AIYAAARLLELLSRQDVPLESLVDGLPKLYSTPEIRIAVPDEQKFAVVKKVADALTAL
                     PGTDVLTLDGVRVTWPDAWALVRASNTQPAVGLRFEAESELRLRSVQSEVQSLLRRAM
                     GELGTEGSSAANAVLAKQPASRLTFFYDLGTPYCYLMAEQVVALSERLAEPVRFVPVV
                     SGRLAAADDLSTVLGPAQRRYLQLDAQRSARRLRLPFHFPSRFPMNSIWALRLCVQVA
                     QKQPTEQQRLVLALLKAYCVDDLDLIDQDVLRRLLLSLSLPADELLHGCSLPEVAALL
                     QHNTEQAIAQGVWKTPCIAVGDELFIGCVPIEQVESALLALRKSASPDPKPDRQGG"
     gene            7999..>8750
                     /locus_tag="PKL17_18060"
     CDS             7999..>8750
                     /locus_tag="PKL17_18060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNK46693.1"
                     /translation="MKGPSDDSKEPPKTVPGDSPQASFAFSAPRPPVRKSPPPDPRPS
                     PPDPPLPPLAVRRLSPERAPASPRPAAADRSDAEPAASAVKVCTVSEIVGRAARLLDE
                     NFASVWIEGEIEGFKRHQVSGNCYLALKDTGARVEAMLRRQVAQSLSFPLRDGLKVRV
                     RGRLTIYKEQGRFQLYIEEVVLSGEGELLLQFEELKARLAKEGLFAEARKRPLPKFPN
                     VIGVATSQSGAALQDILKVATRRGRVQILVAN"
BASE COUNT         1626 a         2507 c         2871 g         1746 t
ORIGIN      
        1 cactcccatc caatcggtac tgctttttcg cttcgccacc gagtagctgc cgctcgccat
       61 tcactttggc gacaaacggc gtccaggtat taaactcaaa cgcttgcagg ttggccgttg
      121 ccgtcgcatg ggccgacagc gcgctgtcac accgtttgat gagcgcatcc acgtctttgc
      181 ccacggcgac cagtttcgca agcggggccc catttagcac cgtgggccaa tttcgcatcc
      241 gggccagctt gtcaccgagc cgcgggcgga caaaacgact cggcttttcc gttccaaaga
      301 gcgcttgccg cagcgtggcg cgacctgtgc ctgtggtttc ggccatcatg aacttgaaaa
      361 gatacagcgc gaactcatcc agttccgagt caagctgatc aagctgggca tcggcatctt
      421 ctgcggcgtc gcgcagcttg atgtgttcca taatggcgga ctcactgagc gtgagcagcg
      481 attcaaacgt ggtgcggtag gtcgtcgccc acgccggggt tcgcaaacga accagcgtat
      541 agcgcagctc gcgccgcagg gcggatggac tcatttggga agacaaagtt gggatttgca
      601 cgttctttcc ttttgttggt tgtgtggatg cacctggccg ggttgcggat tgcggcctgt
      661 tgcaactggg ataccaaccc acggagccgc ccctttttgt ggtttgggaa tgggaaccta
      721 tccgccgatt caggggattg gaaacggacg tgctgtcacg cggccagcga cgaaaagaaa
      781 tgctctggca ggttcgttct gcgggcctgc tgaacgcgat tttttctttg cggaatggac
      841 cgaaagattg gtcctacagc gtgtcttggg gctcatttgg ccgtccggcg ggccggacaa
      901 caatcggcag accggacagc ggtccggatg ggcggactct tttggatttg caaaagctgc
      961 tgggtccgca gtccggacag gtcagaacga caccacgcag ccaaggccgg ctggcggcca
     1021 cctgcacaaa agggaaaact ggggctgggt cacacggccc aagtggtgat gggaaagtcg
     1081 atcaggtgac ggaagacggg ccgcggcggg attaggaaag gaccggcaga ctgcccggac
     1141 gttgacgacc ggcgcgggga tcattttcgg cggcaagctg accgcatgcg gcgtcgatgt
     1201 catcgccgcg ggtcttgcgt acatacgtgg taaagcccag acgcatgcac tcggcttgga
     1261 acgcggcgac ggttttttgt tccggacgtc gataggacga gtgcgggtgc ggattccacg
     1321 ggatcaagtt cagcttggtg ggaatcccgc gcagaagctc tggcagtcgc cgcgcatctt
     1381 cgatgctgtc gtttacgccc ccaagcagca cgtattccac cgtgatgcgc tgccgacgat
     1441 cgagcgggaa acgtcgcagc gccgaaagca ggacagacag cggatacttg cgattgatcg
     1501 gcatcaccac atcgcggact tcatcggtgg tggcgttcag tgaaacggcc aagttaacgc
     1561 ccagaccttc cctcgcgagt ttttcaatct gcggcacaag tcccgatgtc gataccgtaa
     1621 ccctccgccg tgaaaagctg cggcccagcg ggtgaagcag tatccgcagc gcacggacca
     1681 ggttgtcata gttgtgcagc ggctcgccca ttcccataaa caccaggttt gtgaccacat
     1741 cgccgccgcc aagccgtgtc ggatcgtcgg cgggcaggct gcgcaaaagc gactctgcct
     1801 gataaatctg ggtcacgatt tcacccgcgg acagattgcg accaaatccc agcttggcgg
     1861 tcgcacagaa atcgcagtcg atggcgcagc cgacttgcga ggacacgcac tgggtcatct
     1921 ttttgcgttc ccaggtgggc gcttcctctt cgtcatcgta ggcatcgagc gtgcggcttt
     1981 gctgaagctg cccggcaatg atttggcgcg catcgtcgtc gggaattagc acgctttcga
     2041 tcagccgatt gtccgctgtt cgcagccgca gcttgcgggt tccatcggcg gcgatttgca
     2101 ctttgtcgat gtcgagctcg gacacttgca ccaagttttc cgcttgcagc cgcgcccgca
     2161 gctttgcgct gacatcggtc atcgcatcca acgtcgtgac gccccgtgca tgaatccagc
     2221 ggaacagctg ctcaccccgg tagcgttttt ccccaagact ttgcataaag gcggtcaggt
     2281 cgtcgtaggt ctgcgcccga agctcgatcg gcgtgccttg taacgacggg ctgtgtgtag
     2341 ttgggtcagc cgggctgggc gcggacgttg aaagtggggt cagcgtgctc atttcgcgcg
     2401 cagtctacca aggaccggcg gaagagaaag atgcaagcag cgggtcgggt ggaatgacgg
     2461 tctagcgcaa aaacagtgcg gcggcgatgc cacccaggca aagcgtgatg acgaccaccg
     2521 agatgatcac caccatcttg tttgatgatc cactggctga cttctgggcc gcagcaattt
     2581 gttctttggt cgcggtcgac attggcaccg tcgcagcggt gctgggcgga gccagaccgg
     2641 caggggccgg cgctttgatc agcgtctggg ccagccgcgg gggaatggct gacgaggagt
     2701 ttcccgctgc accaccaccc gaagtcgaag acggcgccgc cttgggtgga ttggatgtcg
     2761 cgttgcgctg agaggatgcg cttgcgctgg cgggccgggg cgggttcgat gtgccagatg
     2821 ctcccgctgc ggttggccag ttgatctggt ctttgctcag atcgacgttg ccggttacat
     2881 cgaatttgtc gccgggctgc cctttgaaga cgggcgcttc ggctgctttt tcgtcgccaa
     2941 atgcgggcct tagcaccacc cgaaacgtgc cttcgaccca gccgccgagc ttggacatcg
     3001 ccgcaatggc gctttccggt tggcccgcga tcacggcgct ttccgctttt ccgagcgtaa
     3061 agcgcacttg tgcaacgtcg ccgctggttc gcagcagctc gacgtctgcc gatagctggt
     3121 tgtctgcgca gagcttgtaa atggccgtca cttggcccga tcgcagcgaa ccggtcagtt
     3181 cgatgcctgg cgtcaggcgc cctttccagt cgggaattcg ctgctcgacg atgaattcac
     3241 cgttggtcca cagcggcaaa gatcgggtgc cctgatcgcc ttcgttttcg atcggctcac
     3301 cgccaatcca catcagatcg agcgtctgat cgccttgctg gaggcgcatg ttgccggtca
     3361 gccgcaggcg ctgacagcgt tcatagagct gacggatgga cgtttctttg agagagccag
     3421 accagcgaaa cgcgctctgt gcagaagaag ctagcgacat gtaaagacaa ccccctgaga
     3481 gcttaccgcc atcgcttcgg cggggtcaat cgctggaccg cgtggtcttc ccggcgcgta
     3541 ggtgtcctgt ggacttgtgc gcacattgcc tcttgcacct agggtgaggt tttcgctatg
     3601 aacgatggct tccgcgcccg actgggtgcc aaaaaaatcg tttcaacact gtcggaggtt
     3661 tcgctgatgc gtcgattcac ccacgttcaa ggaatgtttc tgggagcggc ctgtggtttt
     3721 ctgctgtcgg cagcaggcgc tggctgcggc ggtgatgacg ggacgatgga cccgaacaac
     3781 atgccgccgg ctccggtctg tgatgccaaa gcggtgacgg gcaccgacaa caaggctgca
     3841 accgacgcgc tgaagcttcc gggcgtcagt ggcaacaaga cctacgttta cgatttcgac
     3901 ggcgacaacc gccaagaaaa ccagctcaag aacctgatca acaccatctc cctgtcgggt
     3961 ctggacatcc aggcttcggt gaacaaggcc gttcagtcgg gcgatgcgat cgttcttatg
     4021 aacctcaaga ccggcgacat catgaaggcc gattgtgcat cggtgacgat gggtctggcg
     4081 aaaggcccgg cgccgatggc accggcaccg aagttcgatg gctccgacac gttccagatg
     4141 ctgccggaca tcacgcccgt atcgctgttt ggcaagatcg atggtggcaa gctcagcacg
     4201 ctggccagca aagagcagcg cagcgacaaa cagaccgagc agcagattca ggtcaacctg
     4261 ccgcttggtg atggcaaaaa cctgccgctc gcgctgcacg gcgcacacct ggaaggcacg
     4321 gtcgtcaagg acggcagcgt gatccgcatc aaggacggcg tgctgcacgg cgttttggcg
     4381 cagaaggaca tcgacgacaa gatcgttccg ctcgtcgcca acctgctgac cgacatgatc
     4441 aacaaagatc cgatgagcga caccgccaag acgatcatcg gtctgttcga gaaccagaac
     4501 gacgcggtca ccaagaagaa gtgcatggac aacgccaagg actgctgcaa gacgaacccg
     4561 acgacttgca agatcctgcc ggcggaagtc aaaaactcgg cagtcggtgg cgtgctgagc
     4621 tcggacgtcc aggtcttcga cgacaacggc aactgggcgc cggtggctgg tggcaagaag
     4681 tacaacggca tgtcggtggg catcggcttc actggcgttt cggccaagtt ctaggctgct
     4741 cgccggatgt gcttagaagc cacgcggtgt tccgggtggc tggtcgtcga gccctagagg
     4801 gtgttatgcc ctctggggct ttcgactttt gggcgattgg tttccgaaga aggagagcgc
     4861 cgatgaacga cgcacattca tatgtagtga ttttggcggg cggcggggga actcgcctgt
     4921 ggccgcacag tcgtcgcagc cggcccaagc agtttttgcc catgctgccc ggcggagaaa
     4981 cgctgatcgg ggcaaccgcg cgtcgcacaa gtgacgtcgt accgatcgat cgcactctgg
     5041 tggtcacgac ggctgaacaa gtaccggagc tgcggcgctg tctacccgac ctgccgatcg
     5101 acaacatttt ggtcgagccg acgggtcgga acaccgcgcc gtgcattggt cttgcggcga
     5161 tggtgctgtg gaagcgagat ccacaggcgg tgatggcggt gctgccgtcc gatcactacg
     5221 tcgcaaatga agccggcttt cgcgccacat tgacggaagc gctggcggtt gcacatgctg
     5281 gccacgtcgt gacgctgggc attttgccga cgcatccaga gacgggttac ggctacatcg
     5341 agctggggcc cgagcttgac gggcagcggg tggcggcggc gttcgtcgaa aaacccaatc
     5401 gccagcgggc cgaagagtac ctgtcgtcgg gacgttacct gtggaactcg gggacgtttt
     5461 tctttccggc caagcgaatt ttggatgaac tgcggacgca gcttccggcg ctggccgaca
     5521 tcctggacga gcttggcaat caccccgagc gaaccgcggt ccgctatccc gaagcgcccg
     5581 cgatttccat cgactatgca gtgatggaga ggcttgggca aaaccaggcc ggggatgcgc
     5641 gggcgattcg cgtgctggcg ggcgattttg gctggaatga tgttggttca tttgctgcgc
     5701 tcgacgcact gcatcctgcc gatgacaaag gcaaccatgt gtttgccagc agcgcgacga
     5761 cgccacgacc tgtgttggtc gatgctgcac gcaacctggt gtggaatgac ggcaagcggc
     5821 tgctggcgct gattggcgtc gaagacctga ttgttgcgac gacggatgat gcggtgctgg
     5881 tgatgccgcg gcagcgggcg cagcaggtca aggacgcggt cacgctgctg caaaaggaag
     5941 ggcggaccga attcctatga acccgctcat gttccgagag tatgacatcc gtggcatcgc
     6001 cgatcgcgac ttgcccgacg atgtcgtatt cgatctggga caggggattg ggacgctact
     6061 tttcgacagc ggcggccagc ggattgcgct cggtcgggac tgtcgactgc attcgccgcg
     6121 cctgcatgcc gcgctgactg ccggacttgt ggccagcggg gccgacgtgg tcgatctggg
     6181 cgtggttcca tcgccgatgc tgtactttgg cgcccacacg ctggatgtcc acggcggggt
     6241 gcagatcacg ggcagccaca atccgcccag cgacaacggt ctgaagatcc ttttgcacaa
     6301 acgatcccaa ggtggtgagc agatccaaaa actgcgccag cttgtggaag cgaaagcgtt
     6361 tcgcaaaggg cagggcagcg tgcgcacgct tgcgctggag agcgagtatc tgacgttttt
     6421 gcagcagcgc ttggccatcg gcgcgcggcg gtttccggtc gtactcgatg cgggcaatgg
     6481 gacggggggg agcgtcgcgc tgccactgtt tcaatcgctg ggttttccga cgctgggact
     6541 tggctgcgaa cccgatggca gatttccgct gcatccgccc gatccgacgg tgccggaaaa
     6601 tttgcgcctg ctgcaagaca cggtgcggcg cacgggcgca gaagtcggct ttgccttgga
     6661 tggcgacgcc gatcggctgg ttgctgtcga ccggcagggc cggatcttgt ggggcgatca
     6721 gctgctcatt ttgctgtcgc gtgccatctt ggccgaacag ccaggggctt cgttcgtctg
     6781 cgaggtcaag tgcagtcgca cggtggtgga tgagatccgg cgggcgggcg gccacccgat
     6841 catgtggcaa gtcggacaca cccgcatcaa agagaagatg gaggagagcg gggctgcgct
     6901 tggggcagag atgtccgggc acctgttctt tgcgcatcgt tatttgggct ttgacgatgc
     6961 gatctatgcc gcggcgcggc tgctggagct tttgtctcgc caggatgtcc cactcgaaag
     7021 cttggtcgat gggctgccca agctgtacag cacgccggaa attcggatcg cggttcccga
     7081 cgagcagaag tttgcggtgg tgaaaaaagt cgccgatgcg ctgacggccc tgccggggac
     7141 ggacgtgctg acgctcgacg gggtgcgggt gacgtggcca gacgcctggg cgctggtccg
     7201 ggcgagcaac acccagccgg cggtgggttt gcgttttgaa gcagagagcg agctccgtct
     7261 gcgatcggtg cagagcgagg tgcagtcgct tctgcggcgc gcgatggggg aacttggcac
     7321 cgaggggtcg tccgcagcga acgcggtctt ggcaaagcag cccgcttcgc ggctgacgtt
     7381 tttttatgac ctgggaacgc cctactgtta cctgatggcc gagcaagtgg tggcgctgtc
     7441 ggagcggcta gcggagcccg tgcggtttgt tccggtggtc agtggcaggc tcgctgcggc
     7501 agatgatctg tcgacagttt tggggcctgc gcagcgtcgg tatcttcagc tggatgcgca
     7561 gcgctcggct cgtcggctgc ggctgccgtt ccactttccg tcgcgtttcc cgatgaactc
     7621 gatttgggcg ctgcggctgt gtgtgcaagt ggcgcagaaa cagccaaccg agcagcagcg
     7681 gctggttttg gcgctgctca aggcgtactg cgtcgacgat ctggacttga ttgatcagga
     7741 cgtgctgcgc cggctgcttt tgtcgctgtc tttgccagcc gatgagcttt tgcacggctg
     7801 ctcgctgccc gaagtggcgg cgctgcttca gcacaatacc gagcaagcga ttgcgcaagg
     7861 cgtctggaaa acgccctgca tcgcggtggg tgacgaactg ttcatcggct gcgtgcccat
     7921 cgaacaggtc gagtctgccc ttttggccct gcgcaagagc gcaagtcccg acccgaaacc
     7981 agatcggcaa ggtgggtgat gaagggtcca agcgacgatt ccaaagagcc tcccaagacg
     8041 gtgcccggtg acagtccgca ggcgagcttt gcgttttcgg cgccgcgtcc gccggtgcga
     8101 aagtcgccac cgccagaccc ccgaccgtcg ccgcccgatc ctccgctgcc tccgcttgcg
     8161 gtgcggcgac tttcgcctga gcgtgcgccg gcttcgccac gacccgccgc cgcggatcgg
     8221 tcggacgcgg aacctgctgc atcagcggtc aaggtctgta cggtcagtga aattgtcgga
     8281 cgggcggcac ggctgctcga cgaaaacttt gccagcgttt ggatcgaagg ggaaatcgag
     8341 ggattcaagc gccaccaggt gtcgggcaac tgctacctcg cgctcaaaga cacgggcgca
     8401 cgggtcgaag cgatgctgcg gcggcaagtg gcgcagtcgt tgtcgtttcc actccgcgat
     8461 gggctcaagg tccgggtgcg cggtcgcttg acgatctata aggagcaagg gcgctttcag
     8521 ctctacatcg aagaggtggt gctgtcaggg gaaggcgagc ttcttttgca gtttgaggag
     8581 ctgaaagcgc ggctcgccaa agagggtctg tttgcagagg cgcgcaagcg tccgttgccg
     8641 aagtttccca atgtgattgg ggtggcaacg tcgcaaagtg gcgcagcgct gcaagacatc
     8701 ctgaaagtcg cgacccggcg cggacgggtg cagatcctgg tggcaaactg
//
LOCUS       DAORGN010000211         8658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3613_length_8658_cov_5.890736, whole genome shotgun sequence.
ACCESSION   DAORGN010000211 DAORGN010000000
VERSION     DAORGN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3613_length_8658_cov_5.890736"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /gene="ptsP"
                     /locus_tag="PKL17_18065"
     CDS             <1..1509
                     /gene="ptsP"
                     /locus_tag="PKL17_18065"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNK46694.1"
                     /translation="VEWALRKTVAEIQQRFDAIDLPYFRERKSDVAFVGEQVLRNLLD
                     QSGGTTWQPPLGAVVVAHDLSPADTVHLHRHQIAALVTDAGGRTSHTSILAQAFGIPA
                     VVGLDDITEAVGTGDLLIVDGNRGEVILCPSPGMIDDYKQQAKSEHARMSQLLAERDA
                     PAETVDGVRVRLLANIELPDEAPLALEYGAEGIGLYRTEFLFLDREEFPREVEHFMHA
                     RGVLRRVAPYEATFRTFDLGVDKVAPFLNRMAFSHTSVGEAEPNPALGLRSLRLCLKE
                     PTFFKSQLRGLLRASVHGTMRIMFPMVSGVEELRQAKELLTECKRELQKEGVPFSESI
                     PIGIMIEMPSAVMVADHLAQEVDFFSIGTNDLIQYSLALDRLNEHVGYLYQPLHPAVL
                     RMVKLTVDAAHAAGKRVGMCGELAGEPLLTVVLLGLGLDDLSMNAASLPLLRRLLRQL
                     SAEKARALATDVLKLPSAAQIEQVVRKRLTDMLGDDPAWTTLTTQRRSGKPA"
     gene            1652..3202
                     /locus_tag="PKL17_18070"
     CDS             1652..3202
                     /locus_tag="PKL17_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46695.1"
                     /translation="MHIRALIGLATVLVSLSGCERRNLDTCRVPDLPCEDGRFCKVAL
                     GASVGKCEATECMQSSDCPTDRPNCGSGGRCYACGNAGDCTRNGNPGICDVGHCVECK
                     TSKDCSDKSRPYCEDQTRSCRACKRHYECDSTPSARDGVCVKDDTLATLPGTQALSAG
                     MCVPQSRVVSVDMTTCSGSCVLQDKLAEVSADKPYLRIGPFTSSATVTLRPVPGLPEL
                     HVMSTLADYSPPDPAAKDKAPNAFLTNPTGTAVSVESGVNVTIEGLLIKDSKLGIACV
                     GSGNPTRVRVQRTLIAQSNVGIQTSAGCELSLDQSWIGEGPRERYTGLANSANLLALD
                     LDGTKFDITNSVFNHNSPAPGQFGGILVRNATASTPGRIVNTSFVRHETASAARKALV
                     LDCNPATTNITIANSLFLNSTPLAGNTYVHAACRGPNQKYIGSDDTSLTGEGNATDLV
                     MSDVFTAPVSLGDMTLKMTADARVRDGGLVQLLDSQSGKNIIPTTDIIGAARSSTKLS
                     LGAFEASR"
     gene            3275..3490
                     /gene="tatA"
                     /locus_tag="PKL17_18075"
     CDS             3275..3490
                     /gene="tatA"
                     /locus_tag="PKL17_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNK46696.1"
                     /translation="MFGLGATELIIILVIVLIIFGAGKLPQIGDALGRSIKNFKKAAD
                     TGNELDVTPKKELGDAGKPESKVEGKA"
     gene            3499..4083
                     /locus_tag="PKL17_18080"
     CDS             3499..4083
                     /locus_tag="PKL17_18080"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNK46697.1"
                     /translation="MAEPRIIKRYSNRKLYDTQSSQYVTLEQIALMIRQGEEVQVLDN
                     SSKEDLTSVTLAQIIFEEEKRQKNFISLGAMRQLIQSGGASLSELAQQAQARVRSVFR
                     KGDVDGKTLEADESSDPLRRQMLSDNPSAVRELLERWQIALEDWQRKVDDRVHGVVES
                     LSPFAALEKEVTRLQKRVDELEARLTPPSPPDAQ"
     gene            complement(4102..4722)
                     /locus_tag="PKL17_18085"
     CDS             complement(4102..4722)
                     /locus_tag="PKL17_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46698.1"
                     /translation="MSDAASRSVRTIVSHWAHNMTLLHNLGISYPGFGYTDDEKKQLG
                     ALAQSVSSLGHIGFGFVNAIYFCALAGFVMGYGAIPLAFLLDPTHQSAGVFFGCMGLG
                     IAIGIGFGVPASMGLTALTFRLLGKEPQSPDVSDDVVAALYRKMHRQLVRAGIVTGVL
                     IGPWVLFGMTKTGGHVIEFLTRVVLTLAPFAFLLLVLSAFLRGAKK"
     gene            4824..5546
                     /locus_tag="PKL17_18090"
     CDS             4824..5546
                     /locus_tag="PKL17_18090"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNK46699.1"
                     /translation="MSCQKAFHLLFALSICSGCGSADDPVTEEPADDAGISSDAMSPV
                     SDAAFDAAVDAGQGTLKGTAALTYYYVASQSDYSGANDTVLCDAQAKPLATVPRAFAD
                     ALAIEGTGRLTDGRLLNIAASCSCVSGMRTCYAVLDPVKYPFGQGVGGRALKPYRSIA
                     VDKSFIPYGTRVYVPALSGVQMPGSYGFVHDGCLSADDTGGAIIGAHIDWFVAEKANY
                     RTLDAKLRLSSVAVYTNAPQCP"
     assembly_gap    5549..5648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5708..7255)
                     /locus_tag="PKL17_18095"
     CDS             complement(5708..7255)
                     /locus_tag="PKL17_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46700.1"
                     /translation="MWKLTRDFYEQRGISAWDSGIVPSYVTSNPFIAQSYAQVVWQYL
                     RDCLSDRGSCRIDPSQPIYIVELAAGHGRFSYLFLKKFLALKNASSLRHLDVRFVMTD
                     FTETNVKSWARQKLFQDYVDQGVLHLGLFDLECDDYIRLYPSGQLLSQESIKNPLIVL
                     ANYIFDSLLQDFFRFESGKINECLVTTRHTEPLSEQPVNPEVMSNFHLDWEMNPVSPE
                     GYYGDALIEQVLAGYQGRLSDTTLVFPVGAMRTIRRLSRMSGNRLLIVSSDKGYTHED
                     ELFYINGQYVQFHGALSTMSNYHAMGQLVQKLGGHYLATAQRQMSLKTVAYLVGGSSE
                     QFADTMAAFRTSMDVFGPYDFYTLLNSVRTAAPNLPLDGVMELMRLAQYDPQVFFDYE
                     KIILDNAHTLSETQRVELTMSLDRTWANFYPLGRDLPFELGRVYLSLKRPREALRYNQ
                     LSMDMFGEHPVTYCNMGICYYQAEDTANAIVCFEKSLQLNPDYGLPKAWLSRIVAEQQ
                     RHGEPHS"
     gene            7863..>8658
                     /locus_tag="PKL17_18100"
     CDS             7863..>8658
                     /locus_tag="PKL17_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46701.1"
                     /translation="MIRSTLSRALATTVVSLSISACSAPDLTLTCPIPPGATPAEAAA
                     ILSSCFGKMDAIPVNTRAKQDVDILFVIDNSPSMSPKQKQLASAIDSFIQKIDQSGSN
                     YHVGVTSTDIGAQQAPNTAFQPGNLNISGCESYKGNDGELQKQACSSRDQSKWSQAAK
                     DACNLTLCKTKLEPTNGDVYLWKQDGQTNAPSNDIIGTFKCIAPIGDAGCGLESPLEA
                     AKRALDGHSTINQGFLRQNSVLAVIFVTDEDDCSVQLTTRAQSNPNS"
BASE COUNT         1871 a         2551 c         2489 g         1647 t
ORIGIN      
        1 gtcgagtggg cgctgcgcaa gaccgtcgcc gaaatccagc agcgctttga tgcgatcgac
       61 ctgccctatt tccgggaacg caaaagcgat gtcgccttcg tcggtgaaca ggtgttgcgc
      121 aacctgcttg atcaaagcgg tggcacgacg tggcagccgc cgcttggggc cgtggtcgtc
      181 gcgcatgact tgtcgcccgc cgatacggtt cacctgcacc ggcatcagat cgcggcgctg
      241 gtcacggacg cgggcggacg gacgtcgcac acttcgattt tggcccaggc ctttggcatt
      301 cctgccgtgg tcgggctcga cgacatcacc gaagcggtgg gcactggcga tctgctgatc
      361 gtcgacggaa accgtggcga agtcatcctc tgcccaagtc cggggatgat cgacgattac
      421 aagcagcagg ccaagtccga gcacgcgcgg atgtcgcagc tgctcgccga gcgggatgcc
      481 ccagccgaaa cggtcgatgg ggtgcgggtc aggctgctcg caaacattga acttcccgac
      541 gaggctccgc tcgcgctgga atatggtgcc gagggaatcg ggctttatcg gacagagttt
      601 ctgttcctcg atcgcgagga gttcccgcgc gaagttgaac actttatgca cgcacgcggt
      661 gtcctgcgac gggtagcgcc gtatgaggcc accttccgga cgtttgatct gggcgtcgac
      721 aaagttgcac cgtttctgaa tcggatggcg ttttcgcaca ccagcgtcgg ggaagccgag
      781 cccaatccgg cactcggtct gcgctcgctg cggctgtgtt tgaaagaacc gacgtttttt
      841 aagtcccagc ttcgcggtct gctgcgagcg tcggtccacg gcacgatgcg gatcatgttc
      901 ccgatggtca gcggcgtcga agagctgcgc caagccaaag agctgctgac cgagtgcaaa
      961 cgcgagctgc aaaaagaagg ggttccgttt tcggagtcga ttcccatcgg catcatgatc
     1021 gaaatgccgt cggcagtgat ggtggccgat catcttgcgc aggaagtcga ctttttctcg
     1081 atcggcacaa acgatctgat tcagtactcg ctggcgcttg atcggctcaa cgaacacgtc
     1141 ggctatctgt atcagccgct gcacccggcg gtgttgcgga tggtcaagct caccgtcgac
     1201 gcagcgcacg cagcaggcaa gcgggtgggc atgtgcggcg agcttgcggg ggaacccctt
     1261 ttgacggtgg tgttgctggg gcttggactt gatgatctgt cgatgaatgc ggcgtcgctg
     1321 ccacttttgc gacggctctt gcgtcagctg tcggcagaaa aagcgcgggc tctcgcgacc
     1381 gatgtgctca agctgccaag tgcggcccag attgaacagg tggtgcgcaa gcggctcacg
     1441 gacatgctcg gggatgatcc ggcgtggacc accctaacga cgcagcgacg cagcggcaaa
     1501 ccggcgtagg caccggtccg gcccccgcag acccggcctg gtggatgccg gcaagttggc
     1561 gcgttttggc gagcccagcc gaagatcctt gcgcgaagct accgcgggcg caaaaatcag
     1621 cggcaggctc gactgagtcg aggatacgca aatgcacata cgtgcgctga ttggcctagc
     1681 taccgtgctc gtttccctgt ccggctgtga acggcggaat ctcgatacct gccgagtccc
     1741 cgacctcccc tgcgaagatg gtcgcttttg caaagtcgcg ctcggcgctt ccgttggaaa
     1801 atgcgaagcc accgagtgca tgcagagcag cgactgtccc acggatcgac caaactgcgg
     1861 atcgggcgga cgctgttatg cctgcggcaa cgccggcgac tgcacccgca atggcaaccc
     1921 cggcatctgc gacgtcggcc actgcgtcga atgcaagacc agcaaagact gcagcgacaa
     1981 gagccgccca tattgcgaag accaaacccg cagctgccgc gcctgcaagc gccactatga
     2041 gtgcgattcg accccgagcg cccgcgacgg tgtttgcgtc aaagacgaca cgctggcgac
     2101 cctgccggga acccaagcgc tgagcgccgg gatgtgtgtg ccgcagagcc gcgtcgtcag
     2161 cgtcgacatg acaacttgct ctggcagctg cgtgttgcaa gacaagctgg ccgaagtgtc
     2221 cgccgacaaa ccgtacctgc gaatcgggcc gtttacgtcg tctgcgacgg tcacgctgcg
     2281 tcctgtgccg ggtctgcccg agctgcatgt gatgagcacg ctggccgact actcgccgcc
     2341 cgaccctgcg gcgaaagaca aagcgccaaa cgcctttctg acaaacccga ccggcaccgc
     2401 ggtcagcgtc gaaagtggcg taaatgtcac catcgaggga ctgctcatca aagacagcaa
     2461 gctggggatc gcgtgtgttg gatcgggcaa cccaacccgc gttcgcgtcc agcggaccct
     2521 catcgcgcaa tcgaacgtcg gcatccagac cagcgccggc tgtgagctat cgcttgatca
     2581 gtcgtggatc ggcgaaggac cgcgcgagcg ctacaccggg ctcgccaact cggccaactt
     2641 gctcgccctg gatctcgacg gcaccaagtt tgacatcacc aattcggtgt ttaaccacaa
     2701 ctcgccagcg ccggggcagt tcggcggcat cttggtccgc aacgcgaccg catcgacgcc
     2761 ggggcgaatt gtgaacacca gctttgtgcg tcacgaaacc gcatcagcgg cccgcaaagc
     2821 gctggtgctc gactgcaacc cggcgaccac caacatcacg atcgccaaca gcctgttttt
     2881 gaacagcaca ccgctcgccg gcaataccta tgtccatgcc gcgtgtcggg gtccaaacca
     2941 aaaatacatc gggtcggacg atacgtcgct gaccggcgaa ggcaacgcga ccgatctggt
     3001 gatgtccgat gtctttacgg cgccggtctc gcttggtgac atgacgctca agatgaccgc
     3061 cgatgcccgc gttcgcgacg gtggtcttgt gcagctcctc gactcacaaa gcggcaaaaa
     3121 catcatccca accaccgaca tcatcggcgc tgcacgctcg tcgaccaagc tgtctttggg
     3181 agcctttgaa gcgagccggt agcctggccc gtgacctttg ttactggctc actttgagcc
     3241 gaccgggtat aaaagcccgg caaggagcac acatatgttt ggtcttggag cgacggaact
     3301 catcatcatt ttggtgatcg tcctcatcat ctttggcgcc ggcaagctgc cccagattgg
     3361 cgatgcactc ggacggagca tcaaaaattt caagaaagct gccgacactg gcaatgaact
     3421 cgacgtcacg cccaaaaaag agctgggcga cgcaggcaag cccgaaagca aggtcgaagg
     3481 caaggcctag gtcccaagat ggcagagccg cgcatcatca agcgatacag taaccgcaag
     3541 ctctacgaca cgcagagcag ccagtatgtg acgctcgaac agatcgctct gatgattcgc
     3601 cagggcgaag aggttcaggt tctcgacaac agcagcaaag aagacctgac gtcggtgacg
     3661 ctggcgcaaa tcatctttga agaagaaaag cggcagaaga acttcatctc gcttggcgcg
     3721 atgcggcagc tcattcaatc gggcggtgcg tcgctgtcag agctggccca gcaagcgcag
     3781 gcgcgtgttc gcagtgtctt tcgcaaaggc gatgtcgacg gcaaaacact agaagcggac
     3841 gaatcgagcg atccgctgcg tcgtcagatg ctaagtgaca atccgtcggc agtgcgcgag
     3901 ctgctcgagc gctggcagat tgcgctcgaa gactggcagc gcaaagtcga tgatcgggtt
     3961 cacggcgtgg tcgaaagtct gtcgccgttt gccgcgctcg aaaaagaggt gacgcggctg
     4021 caaaagcgcg tcgatgaact ggaagccaga ctcacaccgc ccagcccgcc cgacgcgcag
     4081 tgagcgccct gccccgcttg gctacttttt ggccccccgc aaaaacgccg acagcaccaa
     4141 aagcagaaac gcaaatggcg ccagcgtcag caccacgcgg gtcaaaaact cgatgacatg
     4201 gccgccggtc ttggtcatgc caaacagcac ccacggcccg atcagaacgc cggtcacgat
     4261 gcccgcccgc accagctggc gatgcatttt gcgatagagc gccgccacca cgtcatcgct
     4321 cacatcaggc gattgcggtt ctttgcccag cagccgaaac gtcagcgccg tcagacccat
     4381 cgacgctggc acgccaaaac caatcccaat cgcgataccc aggcccatgc agccaaagaa
     4441 cacgcccgcc gactggtgcg tcgggtcgag caagaacgcg agcggtatcg cgccatagcc
     4501 catgacaaat cccgcgagcg cacaaaagta gatcgcgttt acaaaaccaa agccgatgtg
     4561 tcccagcgag ctgacggact gggccagcgc gccaagctgt ttcttttcat cgtcggtata
     4621 gccaaaccct ggatagctaa tcccgaggtt gtgcaaaagc gtcatgttgt gtgcccagtg
     4681 ggacacgatg gtgcgaaccg aacggctggc ggcgtcactc atggaaaaca ctccgtcgac
     4741 aggctggatc catcctacgt gagcgggcgc aagaaagcac caagcgagcg cccctccttc
     4801 gcgcctggtg taggctggcg gccatgtcgt gccagaaggc attccacctg ctgtttgcac
     4861 tgtccatttg ttccggctgc ggttcggcgg acgatccagt gaccgaagaa cccgcagacg
     4921 acgcaggcat ttcttcggat gcaatgagtc ccgtttccga cgctgcattt gatgccgccg
     4981 tcgacgctgg acagggaaca ctgaaaggaa ccgcggcgct tacgtactac tatgtcgcca
     5041 gccagagcga ctattccggt gcaaacgata cggtgctctg tgatgcccag gccaagccgc
     5101 ttgcgaccgt tccacgcgcg tttgcagatg cgctcgccat cgagggaacc ggacggctta
     5161 cggacggcag actgctgaac atcgcagcgt cgtgtagctg tgtatcggga atgcgaacct
     5221 gttacgccgt gctcgatccg gtgaagtatc catttggaca gggcgtcggt ggtcgtgcac
     5281 tcaagccgta tcgttcgatt gccgtcgaca aaagcttcat tccttacgga actcgggtct
     5341 atgtccccgc gctttccggt gtccaaatgc cgggcagcta tggttttgtc cacgacggat
     5401 gtttgtccgc cgatgacaca ggcggtgcca tcatcggtgc ccacatcgac tggtttgtgg
     5461 cagaaaaagc gaactaccga acgctcgacg ccaagctacg actgtcatct gtggcggtct
     5521 acacaaacgc gccacagtgt ccgtaggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnntc gtttgggagt cgcttaggat tcgtttggga gtcgctcagg aatcgcttgg
     5701 gaaccgctta ggaatgcggc tcgccgtgac gctgctgctc tgccacgatt cgcgaaagcc
     5761 acgctttggg caggccataa tcaggattga gctggagcga tttttcgaag cacacgatcg
     5821 cgttcgcggt gtcttcggcc tggtagtaac agattcccat gttgcagtac gtgaccgggt
     5881 gttcgccgaa catgtccatc gacaactggt tgtaccgcag tgcttcgcgt ggacgcttca
     5941 gcgacagata gacacgcccc agctcaaacg gaagatcccg accaagcgga tagaagttgg
     6001 cccaggtccg atccaggctc atcgtcagct ccacacgctg ggtttcgctg agcgtgtggg
     6061 cgttatcgag gatgatcttt tcgtagtcaa aaaagacctg cggatcgtac tgcgcaagcc
     6121 gcataagctc catcacaccg tccagcggaa ggttcggagc cgccgtccgc acactgttta
     6181 ggagcgtata aaaatcatac ggtccaaaca catccatcga cgtgcggaaa gccgccatcg
     6241 tatcggcaaa ctgttcgctc gatccaccga ccaggtaggc caccgtcttc aggctcatct
     6301 gccgctgcgc cgtcgccagg tagtgtccac ccagcttctg cacgagctgt cccatcgcgt
     6361 ggtagttaga catcgtggac agtgcgccgt gaaactgcac atactgtcca tttatgtaaa
     6421 acagctcatc ttcgtgggtg tatcccttgt cggaagatac gatgagaagc cgattgccgg
     6481 acatccgcga cagacggcga atcgtccgca tcgcaccgac gggaaatacc agcgtggtat
     6541 cgctcagtcg accttggtat ccggccagga cttgctcgat gagcgcgtct ccgtaatagc
     6601 cttccggact gactggattc atctcccaat cgagatgaaa gttcgacatc acttccggat
     6661 tcaccggctg ttccgaaagc ggctcggtat ggcgcgtcgt gaccaagcac tcgtttatct
     6721 tcccgctctc aaagcggaag aaatcctgta gcagcgaatc aaaaatgtag ttcgctagca
     6781 cgatcagcgg attcttgatc gactcctgcg aaagcagctg ccccgatgga tagagccgga
     6841 tgtagtcatc acactcgagg tcgaagagcc ccaagtgaag gacgccttga tcgacgtagt
     6901 cctggaacaa tttctgccgc gcccacgact ttacgttggt ctctgtaaag tcggtcatca
     6961 caaagcggac gtcgagatga cgcagcgaag acgcattttt tagcgccagg aatttcttta
     7021 ggaaaagata ggaaaagcgc ccgtgaccgg cggcaagctc gacgatgtag atcggttggc
     7081 ttgggtcgat gcggcagctt ccgcgatcac ttagacagtc gcgcagatac tgccagacga
     7141 cctgggcata ggactgcgcg ataaaaggat tgctggtgac gtaggaagga acgattccgg
     7201 agtcccaagc actgattccc cgctgctcat agaagtcccg cgtgagcttc cacatgatgg
     7261 actgcgagaa gcgctttcct tgctccagaa caaatggagg tggcggtgca gcctcagtcg
     7321 tgttcgcagt ttcctcactc atcatcgtct ccagtggtcc tgatcttgtg ggtcttaggg
     7381 gtctttggga agcaatctgt acaactgtca gatcgccata tcttgtcatg tggcgcggaa
     7441 gctcgtacag gcaaatcagc gaaaagacgc catttttgtg tccgtgggcc taatttccga
     7501 cgaaaatgga gtgtcttccc ttgtgattcc tagcccgttc ctaccccaaa cagagggctc
     7561 ccaaacgcat gaaggaaggt gtctgtagga tgccatccca gatcggattt ggcatcccta
     7621 cagatgcaga cattcgccaa attttccgac ggaaaatcgc caaaattgac gacgaccatg
     7681 ccttcctaag tgcgcgaaac agaacagctc tgggcagtac ggaaaatcta gattcaactg
     7741 cgatagacgt tgtaaggtct ttgatacgat cgggacaaag tatctagacc atgtgcagtg
     7801 gttcctacgg gagtgccgta gggctctgca acgatgactt ctatgggggc aaggagaatc
     7861 aaatgatccg ctcgacgctt tcacgcgcac tagcgaccac cgtagtctct ctttcaattt
     7921 cggcttgttc cgcgccagac ctgacactga cgtgtccgat tcctcctggt gcaacaccag
     7981 ccgaagctgc tgcgattctg tcttcttgct ttggcaagat ggatgccatt cctgtcaaca
     8041 cccgcgccaa gcaggacgtt gacattctgt tcgttatcga caactcgccc tcgatgtcgc
     8101 caaagcagaa acagctcgcg agcgccatcg acagcttcat tcaaaagatt gatcagagtg
     8161 gatcgaacta ccacgtcggt gtcaccagca cggacatcgg tgcgcagcag gcgccgaaca
     8221 ccgcatttca gcccggcaac ctgaacatct cgggctgtga aagctacaag ggcaacgacg
     8281 gtgagctgca gaagcaggca tgttccagcc gtgatcagag caagtggtcg caggccgcca
     8341 aagacgcctg caacctgacg ctgtgcaaga ccaagctgga gccgaccaac ggtgacgtgt
     8401 acctgtggaa gcaggacggc cagaccaacg cgccgagcaa tgacatcatc ggtacgttca
     8461 agtgcatcgc ccccatcggt gacgcgggct gcggtctgga atcgccgctc gaagccgcca
     8521 agcgcgcact cgacggtcac agcaccatca atcagggctt cttgcgccag aactcggtgc
     8581 tcgcggtgat cttcgtgacc gacgaagacg actgctcggt gcagctcacc acgcgcgccc
     8641 agagcaaccc gaactcga
//
LOCUS       DAORGN010000212         8570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3680_length_8570_cov_5.384263, whole genome shotgun sequence.
ACCESSION   DAORGN010000212 DAORGN010000000
VERSION     DAORGN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_8570_cov_5.384263"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="PKL17_18105"
     CDS             <1..1848
                     /locus_tag="PKL17_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNK46702.1"
                     /translation="PRGGRPLPPMPLKAEPTSMDMPSGDAMGDGEKAEPAAAPPPPAP
                     SPTSRPPLPPPPGRLSVKPPALDDAPARPTVSNEALREEKMARLFGDKPGGLSPLDRP
                     VVPQDRISDKATMMGGEAPAPKAPPPAVTASPSADTPVPAAVSPSASTNPPLPAAPVS
                     PPPVSAAPASAPPPSASPATAPSPLRPPLSRPAPAAAPASSPLAAARPLPRPSTAPVR
                     AAATVGPSSDDPQRKRHFEALSQVVNKALDQLSFQHATPEQLADAASRLERVVKEQSE
                     KIFPLPIGLDSDGLAKQAMAELVGAGPLDELLNDESVFEVAVAGHDRIFVDRGTGQSL
                     SERLFTSPAAVHRAIHRLVSKAHGKLDQGLLEIRLENGVLLQAALPPFARVPSLVLKR
                     VRKSGGRLGDLVSQNVLSAAMSEFLEVCMRERRNLIVSGYGRLVMLGALGGATAAGSR
                     VITVEPVSELDLSGTNSAWIGLFARGPEMRQVIGQAVRMHPDHLLITDVRGPEAFEVS
                     ACLAGGQGVVVGVDAASAREALGRIESLARLSGESPSRKALREELANAVNLAVHVGRT
                     AAGAYRVMEISEVTLAEEGGIDLIPVFTFKPEGGDGQFVATGHVPAFAG"
     gene            1871..>2247
                     /locus_tag="PKL17_18110"
     CDS             1871..>2247
                     /locus_tag="PKL17_18110"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46703.1"
                     /translation="MWRIIATHTREGREIARKEIDVGEVTIGRDADRGLVLQSASVSR
                     RHCKIRVDHTGATIMDEGSANGVLVNGVRVAPNSPTPIGPGVNVEIAEFRITVEPVMM
                     QQPMMGMPGMAPMGQPMMGMPGMG"
     assembly_gap    2248..2347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2348..3379
                     /locus_tag="PKL17_18115"
     CDS             <2348..3379
                     /locus_tag="PKL17_18115"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46704.1"
                     /translation="GMPGMMPMSGAPVGGMPMGGPPAPSVDLVRLIAEGGQFNGRIFD
                     LPPVAEMTVGRGVGNDLVLDDQSMSRKHAKIKRLGNGRIEVEDLNSSNGTYVNGRKIA
                     TAQCGPGDTVRFGELSFRIDGAGGSSRAGQGIESGAGIAAAPPWAKPVFFALAGATAL
                     VWLLWIFKLAVPGGASKADVEKALKQKEAEASEKITAAKDALDKSDFAKAKDLAEQGL
                     ETDPANLDGIRIRAQASRAIDDESKHRQGVTELEKNNPDGFTAALRIYDSMSLDSKKR
                     TELGSALRNKLIPGGEEFCRKKLYRECFDALCAAGRLGGEKLNANTVKLLKDAEKRAK
                     VPAPCPHVK"
     gene            3418..4386
                     /locus_tag="PKL17_18120"
     CDS             3418..4386
                     /locus_tag="PKL17_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46705.1"
                     /translation="MPFFESVRLALSRHPVGVHDLAAGASPSVLKALAERLPTLAAAD
                     FREFLSTWNGGSMFHESLVLRPAAQIEALPSGHFLIGESSDGTLWLDRDGRVLCVDPE
                     EPDPMLAGQHFADWIDVVMAREALLLDRDGEFRDVFEEEDGVLRAEVRRKRAQIGRKR
                     DPEASLFLLELAELALEDGDDGKARELLEGAVSSGGPAGPAWELLAALQRQAGDELAA
                     HESYLRAAQSALSNPLRAMRFLEAAQLAPTKAAALVEAATKADPDLADRLLTQVRESL
                     ADQQIDEAKQLVAKLQLLSGRLVPASSSELARIERDLRSRDALRVI"
     gene            4393..5205
                     /locus_tag="PKL17_18125"
     CDS             4393..5205
                     /locus_tag="PKL17_18125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK46706.1"
                     /translation="MRGQFSSSWGRALFLLLRLLVVLLLLGFLLHLVSPLWFGLVSAL
                     LLLVTVVGNFVAAAGVFGNVLLAGPPDKPQVALTFDDGPDPETTPQVLDVLSQHGAHA
                     TFFVIGERAQRHPELIKRIVAAGHQVENHSHRHSWATAFGSTPKLVRELGLAQTAIFD
                     AAGKRPRFFRAPIGILSPPVVAAAQTLGLTIVGWTAKARDGWASTSVDAACRRLVRAL
                     RPGAILLLHDAGEQLSAKLPSRPTIAPAVLAVLLPKLAARGLSAVTLDELCR"
     gene            complement(5202..6803)
                     /locus_tag="PKL17_18130"
     CDS             complement(5202..6803)
                     /locus_tag="PKL17_18130"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46707.1"
                     /translation="MKTLVFAVASLIGSFAAARPVDPSLVKVSRLLYENRIEEADDAL
                     RPIESSRATDPSVQVLRGEIQFQLGSYDDAAKTLRKAVADSRLPTTELADARSLLDLA
                     TATADATRGLSEKPSPSGHFVFRYRRGKDEVLIPYASAALDQAWAALAQDFADPADPE
                     AAAPKHPIRVEIYEEIADLARVSTLTLREIETSGTIALCKWNRLMIVSPKALLRGYPW
                     LDTLTHEYTHYIVSRVGRGQVPLWIHEGLAKFEEKRWRGVSGGSLSPAMEQLLSTALA
                     KKRLITFEQMSPSMAKLPSQEDTALAFAEVFTFVEYLHSRGGFAGIRALVKGLSEGLV
                     DSKALQTAFGVSFDELDHGWRASLRNRKPKTKLGPFAEKLRFQKSGSKATAAEEDESG
                     DISDPKVRGFVRLGGLLRSRGRLPAAAFEYEKAATQAGTPHPLIALKLGRTYLDLNDA
                     EKAVLALEPASELYPEWAGLQAALGTAYLRKGDSAKAEAALEAAIATSPFDPNVHCGL
                     KAMYEQSKNPKLAEASQACLLLSGR"
     gene            complement(6818..>8570)
                     /locus_tag="PKL17_18135"
     CDS             complement(6818..>8570)
                     /locus_tag="PKL17_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46708.1"
                     /translation="VQLLADRIDVLRGLTREPKDQTDLPTDVADRVQATHRKSETFLL
                     LIGRVQQDSARGEKPAKDLLAALQEIADRLGKLNQEEATLLQDLRKAIAKGAPRPTRN
                     FLREVLLENERHVAEMERDVLHLDDLIGRQRLEELLSIGDEMAQLRDRMRQLLAEHKK
                     HPTDALRKELERELRAFERRIAELAEKARALSDEVPDEFMNREALGQSDMQSRIDKLR
                     DMLQKGELQRAQEELERMSQSLDQLVRGMEQSLQGYRRERFSAEEKALSELENQLADL
                     NHDQQKLKQRTEEVRQSASARARQLLRDRADSLSRRLLPDVQKLRRLLEDVDVDPLGP
                     WGSDEMAKVQQRLDDVLRMLEQGDVEEAKAMAQEAEQSLGRIEEELRAEEQAARWGQR
                     QRLAKSRSKAEQGKNIAHEMASELDRALPRPEDLMNPTERRQLGELRAGQEQLRKRTQ
                     DFLKDLQKRARDTKDAPQLERLSEDANGVVKKAGGFMEQAESELKKLQPRSASSSQGQ
                     AMDQLSQLRKEMQNARRPHSDGMGMRSEREPVKIPGADEYRPPKEFRKDILDAAKREP
                     PTEYRDQVRRYYEELIQ"
BASE COUNT         1406 a         2596 c         2702 g         1766 t
ORIGIN      
        1 ccgcgtggag gccgtccgct gccgccgatg ccactgaaag cagaaccgac gagcatggac
       61 atgcccagcg gcgatgcgat gggggacggc gaaaaagcgg aacctgccgc ggcacccccc
      121 ccgccggctc cttcgcctac gtcgcgtccg ccgctgccgc cgcctccggg tcgcttgtcg
      181 gtgaaaccgc cggcactgga tgatgctccg gcacgtccga cggtttcgaa cgaagcgctg
      241 cgcgaagaaa aaatggcgcg tctgtttggc gacaaaccag gggggctcag cccgcttgat
      301 cgcccggtgg tgccgcagga tcgcatctcc gacaaagcga cgatgatggg tggtgaagca
      361 ccggccccga aagcgccgcc cccagctgtg accgcttcac cgtcggctga cacgccggtt
      421 ccggcggctg tttcaccgag cgcttcgacc aatccgcccc tgccggcagc cccggtgtcg
      481 ccgcccccgg tgagtgcggc tccagcttcg gcaccgccgc cgtcggcctc tcctgcgacg
      541 gctccgtcgc cactgcgtcc accgctgtca cgtccggctc ctgctgcggc acctgcgtcg
      601 tcgcccctgg cggctgcccg accgcttcca cgtccgtcga ctgctcccgt ccgggcggct
      661 gcgaccgtgg gtccgtcgag cgacgatccg cagcgcaagc gtcactttga agcgctgagt
      721 caggttgtaa acaaagcgct cgatcagctg tcgttccaac atgccacgcc ggaacagctt
      781 gccgacgcag cgtcacggct ggaacgggtc gtcaaagagc agtccgaaaa aattttcccg
      841 ctgccgatcg gtctcgattc cgacggattg gccaagcagg cgatggcgga actggtgggt
      901 gccggtccgc tcgatgagct gctcaatgat gagagcgtgt ttgaagttgc cgtcgctggt
      961 cacgatcgga tttttgtcga tcgcggcacc gggcaaagcc tcagcgagcg tttgttcacg
     1021 tcgccggcgg ctgttcatcg tgcgatccat cgactggtca gcaaggccca cggcaagctc
     1081 gaccaaggcc tgctcgaaat tcggctggaa aacggtgtgc tgctacaggc cgcgttgccg
     1141 ccgtttgcgc gggtgccgtc gctggtgctc aagcgagtac gcaagtcggg cggacgtctg
     1201 ggcgatctgg tcagccaaaa tgtgctgtcg gcggcgatgt ccgagtttct tgaagtctgc
     1261 atgcgcgagc gtcgcaacct catcgtttcg ggctatggtc gtttggtgat gctcggggcg
     1321 ctcggcggtg cgactgcggc gggttcgcgc gtgatcaccg tcgaaccggt gagcgaactg
     1381 gatctgtcgg gtacaaactc ggcgtggatc ggtcttttcg cgcgcggtcc cgagatgcgc
     1441 caagtcatcg gccaagccgt gcggatgcat cccgatcatt tgctgatcac cgacgtgcgg
     1501 gggccggaag cgtttgaagt gtcggcctgc ttggctggcg gccagggcgt cgtcgtgggt
     1561 gttgatgcag cgtcggcgcg tgaggcgctc ggtcggatcg aatcactggc gcggctgtcc
     1621 ggcgaatcgc cgagccgcaa ggccctgcgc gaggaacttg caaacgcagt aaacctcgcg
     1681 gtacatgtcg gacgcaccgc cgccggagcc tatcgggtca tggagatttc cgaagtcaca
     1741 ctagctgaag agggtggcat cgatctgatc cctgtgttta cgttcaaacc cgagggtggc
     1801 gacggacaat ttgtagccac tggtcatgtg cccgcttttg cgggataacc ctctgttagg
     1861 ttgagcttct gtgtggagaa tcatcgccac ccatacccgt gaaggccgcg agatcgcccg
     1921 caaggagatc gacgtcggcg aggtgaccat cggtcgtgat gcggaccgag ggcttgtgct
     1981 gcaatcggcg agcgtgtcga ggcggcactg taaaatccgc gtcgaccaca ccggtgcgac
     2041 catcatggac gaaggcagcg ccaacggcgt cctcgtcaat ggggtacgtg tggcgccgaa
     2101 ctctccgacg ccgatcggcc ctggtgtaaa tgtcgagatt gctgaatttc gaatcaccgt
     2161 cgagccggtg atgatgcagc aaccgatgat gggcatgcca ggcatggccc cgatggggca
     2221 gcccatgatg ggtatgccag gcatgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnggg atgccaggca tgatgccgat gtcgggggct cccgtcggtg gaatgccgat
     2401 gggcgggccg ccggctccgt cggttgatct ggtccgtctg atcgccgaag ggggtcagtt
     2461 caatggccgc atttttgatc tacctcccgt cgcagaaatg acggtgggac gcggcgttgg
     2521 caacgatctg gtgcttgatg accagtcgat gtcgcgcaag cacgccaaga tcaagcggct
     2581 cggaaacgga cggattgagg tggaagacct caactcctcg aatggcacct acgtcaacgg
     2641 tcgcaagatc gccacggcgc agtgtggacc aggggacacc gttcgtttcg gcgagctgtc
     2701 gttccgcatc gacggggcag gtggcagcag tcgcgccggg caaggcatcg aaagcggtgc
     2761 tggaattgcg gcggcgcctc cgtgggccaa gccggtgttc tttgcgctcg cgggtgcgac
     2821 ggcgctggtc tggctgttgt ggatcttcaa actggcagtg cccggcggcg cgtcgaaagc
     2881 ggacgtcgaa aaggcgctca agcaaaaaga agccgaggcc agcgagaaaa tcaccgcggc
     2941 caaagatgcg ctcgataagt ctgactttgc caaagccaag gatctggccg agcaaggtct
     3001 ggaaaccgac ccggcgaacc ttgatggcat tcgcattcgc gcccaggcca gccgggcgat
     3061 cgacgacgaa tcgaagcatc gccaaggcgt caccgagctg gaaaaaaaca atccagacgg
     3121 cttcacggcg gcgctgcgga tttatgactc gatgtcgctc gacagcaaaa agcggacgga
     3181 gctgggatcg gcgctgcgca acaagctgat tcccggtggt gaagagttct gccgcaagaa
     3241 gctgtaccgc gagtgtttcg atgcgctctg cgcggcgggt cgtctcggtg gtgagaagct
     3301 caacgccaac accgtcaagc tgctcaaaga cgccgaaaag cgcgccaaag tcccggctcc
     3361 ctgcccacac gtcaagtagc cttcgcgccc cgttctgcgc tagccttccc gcttcccatg
     3421 ccctttttcg agtctgttcg tctcgcgttg tcgcgacatc cggtcggcgt tcatgacctg
     3481 gctgccggcg cttctccttc tgtgctgaag gcacttgccg aaaggctgcc gacgctcgct
     3541 gctgccgatt ttcgcgagtt tctgtcgacg tggaacggtg ggtcgatgtt ccacgagtcg
     3601 cttgtcttgc ggcctgctgc ccagatcgaa gcgctgccgt cgggtcattt tctgatcggg
     3661 gaatcgagcg acgggacgct gtggctggat cgcgatggcc gggtgctctg cgtcgatcca
     3721 gaagaacccg atccgatgtt ggccggccaa cattttgcgg actggatcga tgtggtgatg
     3781 gcgcgagaag cgctgttgct cgaccgcgac ggtgaatttc gcgatgtctt cgaggaagaa
     3841 gacggtgtgc tgcgagccga agtgcgacgc aagcgggccc aaatcggacg caaacgcgat
     3901 ccggaagcgt cgcttttttt gctagagctg gccgagctgg cgctagaaga cggcgacgac
     3961 ggaaaagcgc gggagctgct tgaaggcgcg gtgtcgtccg gcggtccggc gggtccggcg
     4021 tgggaacttc tggcggcgct tcagcggcag gccggcgatg agcttgccgc ccacgagtcg
     4081 tatctgcgcg cggcgcaatc ggcgctgtcg aatccgctgc gggcgatgcg ctttttggaa
     4141 gcggcgcagc ttgcaccgac gaaggcagcg gctctggtgg aggcggcgac caaggccgac
     4201 ccggacttgg ccgatcggct gctcacccag gttcgcgaaa gcctggcgga ccagcaaatc
     4261 gacgaagcga agcagcttgt ggccaagctc cagcttcttt ccggacggct tgtaccggcc
     4321 agcagcagcg aacttgcgcg gatcgagcgt gacctgcggt cccgcgatgc gctgcgcgtg
     4381 atctgattgg cgatgcgcgg gcagttttca tcgtcgtggg ggcgggctct gtttctgctg
     4441 ctgcggcttt tggttgtgct cttgctgttg ggctttttgc tgcatctggt ttcgccgctg
     4501 tggttcggtt tggtgagcgc tttgctcttg ctcgtcaccg tggttggcaa tttcgtcgcc
     4561 gccgctggtg tatttggcaa cgtgctgctc gcggggccgc ccgacaaacc gcaggtggcg
     4621 ctgacgttcg acgacggacc tgatccagag acgacgccgc aggtgctgga cgttttgagc
     4681 cagcatgggg cccacgcgac gttttttgtg attggcgagc gagcgcagcg acatcccgag
     4741 ctgatcaagc ggattgttgc cgcaggtcac caagtcgaaa atcactcgca ccgacattcg
     4801 tgggcgacgg cgtttggatc tactccaaaa ctggtgcgcg aactgggatt ggcgcagacg
     4861 gcgatcttcg atgcggcggg gaagaggccg cgctttttcc gggcgccgat tggcatcctg
     4921 tcgccacccg ttgtcgcggc ggcgcagacg ctggggctta cgattgtcgg ttggacggcc
     4981 aaagcccgcg acggctgggc gagtacgtcg gtggatgcag catgcaggcg acttgtccgg
     5041 gcgctgcgac cgggggcgat tttgctttta cacgacgctg gcgaacagct gtcggcaaag
     5101 ctgccatctc gtccgacgat tgcgcccgcg gtgctcgcag ttcttctgcc aaaactggct
     5161 gcgcgcggtc tttcggcggt gacgctcgac gaactgtgtc gctagcgacc gcttagcaaa
     5221 agacaggctt ggctggcctc ggcaagcttg ggatttttgc tctgctcgta catcgctttg
     5281 agcccgcagt ggacgttcgg atcgaacggg ctggtggcaa tcgccgcttc gagcgccgct
     5341 tcggctttgg cggagtcgcc cttgcgcaga taggcagtgc caagcgcggc ttgcagaccg
     5401 gcccactcgg gataaagctc gcttgccggt tccagcgcca agaccgcttt ttccgcgtcg
     5461 tttaggtcca ggtacgtccg tcccagcttg agcgcaatca gcggatgcgg tgtgccggct
     5521 tgcgtcgcgg ctttttcata ttcaaacgct gccgctggaa gtcgtccgcg tgaacgcaga
     5581 agccctccga gcctgacgaa gccgcgcact ttgggatcgc tgatgtcgcc cgattcatct
     5641 tcctcggctg ctgtggcttt gctcccggat ttttggaagc gcagcttttc ggcaaatgga
     5701 cccagcttgg ttttgggttt gcggttgcgc agcgaggcac gccagccgtg atccagctcg
     5761 tcgaacgaca cgccaaacgc ggtctgcagc gccttggaat cgacgagacc ttccgacagt
     5821 cccttgacga gcgcacgaat gccggcaaaa ccgccgcggc tgtgtagata ttcgacaaac
     5881 gtaaacactt cggcaaacgc cagcgccgtg tcttcctgcg acggcagctt ggccatcgac
     5941 ggcgacattt gttcaaacgt gatcagccgt tttttcgcga gcgccgtcga caaaagctgt
     6001 tccatcgccg ggctcaggct gccgccggaa acgccgcgcc agcgtttttc ttcgaacttg
     6061 gccaggcctt cgtgaatcca cagcggaact tggccgcgtc cgacgcgcga cacgatgtaa
     6121 tgggtgtatt cgtgcgtcag tgtgtcgagc cacggatagc cgcgcagaag cgctttgggc
     6181 gacacaatca tcagccggtt ccacttgcaa agcgcgatcg tccccgaggt ttcgatttca
     6241 cgcagcgtca gcgtcgacac ccgcgccaga tcggcaatct cttcgtaaat ttcgacgcgg
     6301 atcggatgct tgggcgccgc cgcttctgga tcggcgggat cggcaaagtc ttgcgcaagc
     6361 gctgcccacg cttggtccag tgcggcactt gcatacggaa tcagcacttc gtctttgccg
     6421 cgacggtaac ggaagacaaa atgaccgctt ggtgacggtt tttcgctcag cccgcgggtc
     6481 gcatccgccg tcgccgtcgc cagatcgagg agtgatcgcg catcggccag ttctgtcgtc
     6541 ggcaagcgac tgtcagcgac ggctttgcgc agcgtcttgg ccgcatcgtc atagctgcca
     6601 agctgaaact gtatttcgcc ccgcagcact tgcaccgacg gatcggtcgc gcggcttgat
     6661 tcaattggtc gcagggcgtc gtcggcttcc tcgatgcgat tttcgtagag cagccggctg
     6721 actttgacaa gcgacggatc gacaggtcgc gccgccgcaa acgagccaat cagcgacgcc
     6781 accgcgaaaa ccaaggtttt caaacacgcg cgtcctgtca ctggatcagc tcctcgtagt
     6841 agcgccggac ttgatcgcga tattccgtcg gtggttcacg cttggccgca tccaggatgt
     6901 ctttgcggaa ctctttgggc ggacggtatt catcggcgcc cggaatcttg accggctcgc
     6961 gctcggatcg catgcccatg ccgtcgctgt gtgggcgtcg cgcattttgc atttctttgc
     7021 gcagttgcga aagctgatcc atcgcctgac cctgcgacga cgaagcactg cgcggttgca
     7081 gttttttgag ctcgctttcg gcttgctcca taaacccgcc cgcttttttg accacgccgt
     7141 ttgcatcctc gctgagccgc tcgagctggg gcgcatcttt ggtgtcgcgc gcccgctttt
     7201 gcaggtcttt gagaaagtcc tgggttcgct tgcggagctg ttcctggcct gcccgtagct
     7261 cgccgagctg gcgtcgctcc gtcgggttca tcaggtcttc cgggcggggc agggcgcgat
     7321 ccagctcgct ggccatttcg tgcgcgatgt ttttgccctg ctcggctttg ctgcgtgact
     7381 tcgcaagtcg ctgcctctgt ccccagcgcg ccgcttgttc ctcggctcga agctcttcct
     7441 cgatgcgccc gagcgactgt tcggcttcct gcgccatcgc tttggcttct tcgacgtcgc
     7501 cttgttccag catgcgcagc acgtcgtcga gccgctgctg aacttttgcc atttcgtcgc
     7561 tgccccacgg cccaagcgga tcgacgtcga catcttcgag cagccgccgc agtttctgca
     7621 catcgggaag cagtcgtctc gacagcgaat cggcgcgatc gcgcaaaagc tgtcgggccc
     7681 gcgcactcgc gctctgccgc acttcttcgg tccgctgctt gagcttttgc tggtcgtggt
     7741 ttaggtccgc aagctgattt tccagttccg aaagcgcttt ctcctcggca ctgaagcgct
     7801 cacgccgata gccttgcagg ctctgttcca tgccgcgcac cagctgatcg agcgactgcg
     7861 acatccgctc cagctcctct tgcgcacgct gaagctcgcc tttttgcagc atgtcgcgca
     7921 gcttgtcgat gcggctctgc atgtcgcttt ggccgagcgc ttcgcggttc ataaactcgt
     7981 cgggaacttc gtcggaaagc gcccgcgctt tttccgccag ttcagcaatt cgccgctcaa
     8041 acgcccgcag ctcgcgctcc agctctttgc gcagcgcgtc ggtcgggtgt tttttgtgtt
     8101 cggcaagtag ctgccgcatc cgatcgcgca gctgtgccat ttcgtcgccg atcgacagca
     8161 gttcttcgag cctttgccgc ccgatgaggt cgtccagatg cagcacatcg cgttccattt
     8221 ccgcgacatg ccgttcgttt tccaaaagca cttcgcggag aaagtttcgc gtcggacgcg
     8281 gcgccccttt ggcgatcgct ttgcgcagat cctgaagcag cgtcgcttct tcctggttta
     8341 gcttgcccag ccgatcggca atttcttgca gcgccgccag cagatccttg gccggttttt
     8401 cgccgcgcgc cgagtcctgc tgcacccgcc cgatcagcag caaaaacgtc tcacttttgc
     8461 ggtgggtggc ctgtactcga tcggcaacat ccgtcggcaa atcggtctga tctttgggct
     8521 cgcgggtgag tccgcgcaac acatcaatcc gatcggccag aagctgtacc
//
LOCUS       DAORGN010000213         8208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3943_length_8208_cov_5.321231, whole genome shotgun sequence.
ACCESSION   DAORGN010000213 DAORGN010000000
VERSION     DAORGN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3943_length_8208_cov_5.321231"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKL17_18140"
     CDS             complement(<1..224)
                     /locus_tag="PKL17_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003170989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46709.1"
                     /translation="MLRAFLPVGYRNLRTDAKNLVGLGRINLLVGPNNSGKSNYIRAL
                     GFLRDMLLCDGGSTSFLQTVEKHGRAEVVNR"
     gene            367..1350
                     /locus_tag="PKL17_18145"
     CDS             367..1350
                     /locus_tag="PKL17_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46710.1"
                     /translation="MKARMQQSRSVRWFLACTVWLGLGLHGQPVRAADEYQQHLKRGQ
                     DLVRTEEYSAALSQFEAAYALRQAPTLLYQIARCHHRLGNTKEAKAFYKRYLSAGDNI
                     DARRKHEVEDSLRQLGEATPTGAVSEPKRGRLVASTNPNMWPAPASSRRGTTDPFEPA
                     GEARPAPSGWVAEPRRPSREADDDEDSAEAPPRPRTRATSSGTAIAGGVLWGASYVPA
                     VVVGSMILIGSNAVSFSSSTTKSLVQGAGGTLLIPIAGPFLSGGLSLSLEWAAPWIFI
                     DAAAQIAGLAMLVAGARSVPVSDDSVSSTLQLTPTPRSVFPDAAPVLSLSY"
     assembly_gap    1480..1579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1686..2573)
                     /locus_tag="PKL17_18150"
     CDS             complement(1686..2573)
                     /locus_tag="PKL17_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46711.1"
                     /translation="MTKKKAAPKSSAKAPSATTNRPPISEPVIASSPAAVSPDPSVFL
                     GEAALEKLRTRLAAVPVESQESPRYNIRLGATAALRLAEEVERALLLDRLRKLHSIGE
                     FDLSLLPLLPDLAHAAWYIRHRLDQASALATEARVPSSLVESASQLRQNMLRVLDFHF
                     GDDPDIEPQLAHIRRGAGHQDTADDLVALAALYKQHEKTLAGTPRYYDKHQAADAQSQ
                     AAAILSALSQDLGRDAAEWVDLQRRISPLLEKSYEEIAQTARYLTRKDPQNETRFPRL
                     FSISRNRPAKKQSASPDPI"
     gene            complement(2658..3272)
                     /locus_tag="PKL17_18155"
     CDS             complement(2658..3272)
                     /locus_tag="PKL17_18155"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNK46712.1"
                     /translation="MKDKRSVKIGILAEDQTDCKTIEVLVGRLLSASGKSASFSHRGK
                     DGCANLRRKAKAWMGELTDVGCAALILIHDLDRNPSNHTLNDEAELRGRLAQIPVPRG
                     VAHHICIPVEEVEAWFWSCPTALERVAPGQGNSRAKPSPHLLPKPKEALIKLSAAQHG
                     GKARYTTGQNPELAALLDLELCASRCPSFRDLRSFVIDSVASAA"
     gene            complement(3262..4359)
                     /locus_tag="PKL17_18160"
     CDS             complement(3262..4359)
                     /locus_tag="PKL17_18160"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46713.1"
                     /translation="MSENESTVSPQRQHAELLAVEVGDWAPLGGDVRLELAGRRTVLV
                     GRNGSGKSALLEGILLSCAEMPEQLLFEFSPYERRKSIEKTRLALLPKQARFEMRDSS
                     GKRLFYSCKPGMDESRHWRPDHPHETEISNGLLEECMVDVLGMHRVLAGVPRQTSLRG
                     KQIVTVPKGNGYCRYLNRDEPRIGRLAEQLVSRYYQFSDGKDALAEIDELGKRIGAWE
                     TLRIDSYERAGDPEQLASVSVDGVDLGLLADGTLRVVELLWEMIRRERQGFRTLLIEE
                     PETGVHPGLLAGLLDTIDAYTVDRQVIISTHSPQVVSWAQPQELRVVTRQEGKTQVRP
                     LSEQQVALFLKYLHDQTLGDFVYSGALDDEG"
     gene            complement(4469..5068)
                     /locus_tag="PKL17_18165"
     CDS             complement(4469..5068)
                     /locus_tag="PKL17_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNK46714.1"
                     /translation="MPSLSFLTPKVQLATFADLLLIPEADRFHEILDGELVKKALPRG
                     EHGVSQAQVVMVLRDFARRKNDPFRPGGWWFATEPTIRLSLHEVVQPDLAGWRRDNLP
                     NRPSGYPVTTRPDWVCEIMCDGDARRRDGLQKRRIYADHGIPHFWLLDTERETLTVLS
                     LTDRGYVEVLCAEKSQQVTAIPFELLPLQVAVLLGEDEP"
     gene            complement(5085..6011)
                     /locus_tag="PKL17_18170"
     CDS             complement(5085..6011)
                     /locus_tag="PKL17_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46715.1"
                     /translation="MVGAPVSNPSQTLAEAVIDGFFSQPLLSLCPQSMLLPVIQKALL
                     LHLGSPLAAEQADALWQWIKIRLSVPGPLRQLLPLPLVTLVRTVLSRPYSVPKPLVVA
                     LLSRPPLRRLQRELMLATLIDYSRKVRSQVGEASQSRGGMLGRLATEAVKKSTSAIGS
                     LAPGVTAAVSDELERQFRARASEFADSAVDDLLSRMAQILTDPTRQTEQRELVLSILD
                     FALDLPAVTLRDELSRLDPLAVAADIRKALRDWLSRPDATEELAALAAPLSPLLQDQS
                     LASLLGPAQPPIRALLVELLSTQLARHLQPPG"
     gene            6149..>8208
                     /locus_tag="PKL17_18175"
     CDS             6149..>8208
                     /locus_tag="PKL17_18175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNK46716.1"
                     /translation="MWRTQKDMVKNLLRPLSGTLALATLLGGVAQAASTDPTKHVSEA
                     TLVLPDLASVQFLGMSGKALLMGGLLVCILGMVFGLTIYNQLKNMPVHRSMREISELI
                     YETCKTYLETQGRFIALLWVFIAAIMAVYFGVFEKLGMAKIITILVFSVVGILGSYGV
                     AWFGIRVNTFANSRAAFASLGGKPFPTYAIPLKAGMSIGMLLISVELVLMLGILLFVP
                     RDYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIA
                     DCTGDNAGDSVGPSADGFETYGVTGVALITFILLAVASEQTQVQLLVWIFAMRIMMVV
                     VSGISYLINETIAKGKYGNAAKMDFEHPLTFLVWLTSFVSVGVTYLISNLLIKDLGDG
                     SMWWKLSTIITCGTLAGAIIPEMIKVFTSTRSGHVREVVTSSREGGASLNVLSGLTAG
                     NFSAYWMGLVFTILMGVAYYVSQSFPSDMMIAPAIFSFGLVAFGFLGMGPVTIAVDSY
                     GPVTDNAQSVYELSLIESVPNVKEEIKKDFGFEPNFETAKEYLEENDGAGNTFKATAK
                     PVLIGTAVVGATTMIFSIIMMLTNHLQDAQSLSNLTLLYPPFLLGLIMGGAVIYWFTG
                     ASTQAVSTGAYRAVEFIKKNIKLEGVEKASVTDSKKVVEICTVYAQKGMFNIFLTVFF
                     STLA"
BASE COUNT         1539 a         2489 c         2515 g         1565 t
ORIGIN      
        1 cggttgacca cctcggccct gccgtgcttt tcgacggtct gcaaaaaaga ggttgagccc
       61 ccgtcacaca gcaacatgtc gcgcaaaaaa ccgagtgccc ggatgtagtt gctcttgcca
      121 gagttgttcg gcccgacaag caggttgatg cggccaagcc ccaccaggtt tttcgcgtcg
      181 gttcgcaggt tgcgataccc aacaggcaga aacgcacgaa gcatggcgca ccctagcacg
      241 tcggggcgcg gtggaaaaat cggtcgcggc agcacaagca gctgaaatcg gctggggcgc
      301 ttgcggtgaa aaccgctggc gtggtcagat gtgcgcgttt tgcagcttcg gcaaagggag
      361 gcttcgatga aagcacgcat gcagcagtct aggtccgtcc gctggttcct ggcttgcacc
      421 gtctggctcg ggcttggtct gcacgggcag cctgttcgcg cggctgatga gtatcagcag
      481 cacctgaagc ggggccagga cctggttcgg accgaggaat attcggcagc cctttcgcag
      541 ttcgaggcag cctatgcact taggcaagcg cctacgctgc tctatcagat tgcacgctgt
      601 catcatcggc tcggaaacac aaaggaagcc aaggcgtttt acaagcgata cctgagcgcc
      661 ggcgacaaca ttgatgcgcg ccgcaagcac gaagtcgaag actcgctgcg acagcttggc
      721 gaagccaccc caacaggggc agtcagcgag ccgaagcggg gtcggctggt cgcatccacc
      781 aatccaaaca tgtggccagc cccagccagc agtcggcggg gaaccaccga tccatttgaa
      841 ccagcggggg aggcacgccc agctccaagc ggctgggtgg cagagccgcg ccgcccaagc
      901 cgggaagcgg acgacgatga agacagcgca gaggccccac ctcgaccgcg gacccgtgcg
      961 acaagttcag ggaccgcgat agcaggcggc gtgctatggg gcgcatcgta tgtccccgcc
     1021 gtagtggtgg gctcgatgat tctgattggc agcaatgccg tgagttttag ctcgtcgacc
     1081 accaagtcgt tggttcaagg ggctggcggc acgctcctca tcccgattgc cggtccgttc
     1141 ctcagtggcg gtctctcgct cagtctcgaa tgggcagccc catggatctt cattgatgct
     1201 gccgcacaga ttgccgggct ggccatgctg gtggccggtg cccgctctgt gcctgtctcc
     1261 gatgacagcg tgtcctcgac gcttcaactg acgccgaccc cgcggtcggt gtttcccgac
     1321 gccgcccccg tactgtccct gtcgtattag taactgccgg atttcccctt ctcgagtaga
     1381 ccctatcgcg tcttcacacc ccttttcccc ttctcgagta gaccctatcg cgtcttcaca
     1441 ccccttttcc ccttctcgag tagaccctat cgcgtcttcn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnt ttccccttct cgagtagacc ctatcgcgtc ttccaccccc
     1621 tctttcccct tctcgagtag accctatcgc gtcttccacc ccctctttcc ccttctcgag
     1681 taggtctaaa tggggtctgg agaggccgac tgcttcttgg cgggacgatt gcgcgagatg
     1741 gaaaacaggc gcgggaagcg ggtttcgttt tgggggtctt tgcgggtgag gtagcgggcg
     1801 gtctgggcga tttcttcgta ggacttctct agcagcgggc ttatgcggcg ctggaggtcg
     1861 acccactcgg cggcatcgcg gcccaggtct tggctgagcg cagacaggat ggcagcggct
     1921 tggctctgcg catcggcggc ttggtgcttg tcgtagtagc gcggggtgcc ggccagggtc
     1981 ttttcgtgct gcttgtagag cgccgccagc gcaaccaggt catcggcggt gtcttggtgc
     2041 ccggcgccgc gacggatgtg ggcgagctgc ggttcgatgt cggggtcatc gccgaagtgg
     2101 aaatcgagca cgcgcagcat gttttggcga agctgcgacg ccgattcgac cagcgatgac
     2161 ggcacccgcg cttccgtcgc aagcgcgctc gcctggtcga gccggtggcg gatgtaccaa
     2221 gcggcgtgcg ccagatcggg caacagcggc aacagcgaca ggtcaaactc accaatcgag
     2281 tggagcttgc gcagacggtc gagcagcaga gcgcgctcga cttcctcggc gagccgcagc
     2341 gccgccgtcg ccccgagccg gatgttatag cgcgggcttt cctgcgattc gacggggacc
     2401 gcagccaggc gggtgcgcag cttttccagg gccgcttcgc ccaaaaagac gctcggatcg
     2461 gggctgacgg ctgccggcga actcgcaatg accggctcgg aaatgggcgg gcggttggtg
     2521 gtggcagacg gggccttggc ggacgacttg ggagcagctt ttttcttggt catgttgtgg
     2581 gtatagcagc ccaattcgcg ctgaccaggg gtccaaggtg attttttggg cagcgcccta
     2641 tcgcgtggac ccaaacgcta cgctgcggaa gccacggaat cgatgacgaa cgaccgcagg
     2701 tcgcgaaacg acggacagcg actcgcgcac aactccaaat cgagaagtgc tgccaattcg
     2761 gggttttgcc cggtggtgta gcgtgctttg ccgccgtgct gtgcggcaga caacttgatc
     2821 agcgcttctt tgggctttgg cagaaggtgg ggggacggct tcgcccgcga gttcccttgc
     2881 cccggtgcca cccgctcgag cgcggtcgga caggaccaaa accaggcctc tacttcttcg
     2941 accggaatgc agatgtgatg ggccacgccg cgcggcaccg gaatttgcgc aagtcgaccc
     3001 cgtagctcgg cttcgtcgtt gagggtgtga ttgctcggat tgcgatccag gtcgtgaatc
     3061 aggatgagcg ccgcacagcc gacatcggtg agttccccca tccaggcctt ggctttgcgt
     3121 cgcaggttgg cgcagccgtc tttgccacga tgagaaaacg aagcgctctt tccgctggct
     3181 gacaaaagtc gcccgaccag aacctcgatc gttttgcagt cggtctggtc ttcggccaag
     3241 atgccaatct tgacgctgcg cttatccttc atcgtcgagc gccccggaat agacaaaatc
     3301 acccagcgtc tggtcatgca ggtatttcag aaacagagcg acttgctgtt ccgacagcgg
     3361 acgaacctgc gttttgccct cttggcgcgt caccacccgc agttcttgcg gctgggccca
     3421 gctcaccacc tgcggcgagt gcgtcgagat gatcacctgc ctgtccaccg tatacgcatc
     3481 aatcgtatcc agcagcccag cgagcagccc cggatgaacc ccagtctccg gctcctcgat
     3541 gagcagcgtt cgaaagccct gccgctcgcg acgaatcatc tcccacagca gctcgacgac
     3601 gcgcaacgtg ccatcagcca atagtcccaa atcgacacca tcgaccgata cggaagcaag
     3661 ctgctccgga tcgccggcgc gctcatagga atcaatccgc aatgtctccc aggcgccgat
     3721 gcgctttccg agttcgtcga tctcagccag cgcgtctttt ccatcagaga attggtaata
     3781 gcgagacaca agctgctccg ccaatcggcc aatgcgaggc tcgtctcggt tcaggtagcg
     3841 acagtagcca ttccccttgg gcaccgtcac gatttgcttt ccacgcaaag aggtctgccg
     3901 aggaacaccc gctaacaccc ggtgcatccc gagcacatcg accatgcact cttctagcaa
     3961 gccgttggaa atctcggttt cgtgcggatg gtctggacgc caatgtcgcg actcatccat
     4021 tcctggcttg caggaataaa acagccgctt gcccgacgaa tctcgcatct cgaagcgagc
     4081 ctgcttggga agcaaggcca gacgggtttt ctcgatcgac tttctgcgct cgtaaggaga
     4141 gaactcaaag agaagctgtt ccggcatctc ggcacagctc agcaaaatcc cttccaatag
     4201 cgcagacttc ccagagccat ttcgacctac caacaccgtg cgccgcccgg ccagttccaa
     4261 tcgcacatca ccgccgagcg gggcccaatc gccaacttcc accgcgagca gttccgcatg
     4321 ctgacgctgc ggagaaacgg tgctctcgtt ctcactcatg cacggaccct aaccccttcg
     4381 cttacaggct ggcaacttgg cggcagcaaa aacggcaaag aaccaccgcg gctccgctcg
     4441 aaagaggaac gcgaagaaaa gcagatcact acggctcgtc ttcgcccagc aggacggcga
     4501 cttgcagggg gagaagctcg aaggggatgg cggtgacttg ctggcttttt tcggcgcaca
     4561 ggacttcgac gtagccgcga tcggtcaggg agagcacggt cagcgtctcg cgctcggtgt
     4621 cgagcagcca gaagtggggg atgccgtggt cggcgtagat gcggcgcttc tggagtccgt
     4681 cacgacggcg ggcgtcgcca tcgcacatga tctcgcagac ccagtcgggg cgggtcgtca
     4741 cgggatagcc gctcgggcgg ttcggcaggt tgtcgcgacg ccagccggcg aggtcgggct
     4801 ggacgacttc gtgcagggac aggcgaatgg ttggttcggt ggcaaaccac cagccgccag
     4861 ggcggaaggg gtcgttcttc ctgcgggcaa agtcgcgcag caccatcacg acctgggctt
     4921 gcgagacgcc gtgctcgccg cgggggagcg ctttttttac aagctcacca tcgaggattt
     4981 cgtggaagcg gtcggcttcg gggatgagca gcaagtcggc aaacgtcgca agctggactt
     5041 tgggcgtcag aaacgaaagc gaaggcatgc gcgaaccgta gcacttagcc tgggggctgc
     5101 aagtggcggg caagctgcgt cgacaacagc tcgacaagca gggcgcggat ggggggctgg
     5161 gccgggccga gcagcgaggc gagcgactgg tcttgcaaaa gcggcgacag cggcgcggcc
     5221 aaggcggcca gctcttcggt ggcatcggga cgggacagcc agtcgcgcag cgctttgcga
     5281 atgtccgcgg cgacagccag agggtcaagc cgcgatagtt cgtcgcgcaa tgtcactgcc
     5341 ggcaggtcga gcgcaaagtc gagtatcgag agcacaagct cacgctgttc ggtctggcgg
     5401 gtcggatcgg tcaggatctg cgccatccgc gacagcagat catcaacggc agaatcggca
     5461 aactcactgg cgcgggcgcg aaactggcgt tccagttcat cggacaccgc cgcggtcacg
     5521 ccgggggcaa gcgatccaat cgccgacgtg ctctttttca ccgcctcggt cgcgagccgt
     5581 cccagcatgc cgccgcgcga ctgcgatgct tcgccgactt ggctgcgcac cttgcggctg
     5641 tagtcgatga gcgtggccag catcagctcg cgctggagcc gccttagcgg cggacgtgac
     5701 aaaagcgcca ccacaagggg cttgggcacg ctgtaggggc gcgacaaaac cgtccgcaca
     5761 agggtcacaa gcggcagcgg cagaagctgg cgcagcggtc ctgggaccga caagcggatc
     5821 ttgatccact gccacagcgc gtcggcctgc tccgcagcaa gcggcgagcc caggtgaagc
     5881 agcagcgctt tttgaatcac cggcaagagc atgctttgcg ggcacagcga cagcagcggc
     5941 tgcgaaaaaa agccatcgat gaccgcttcg gccaacgtct gcgagggatt ggaaacaggc
     6001 gcaccgacca tcggcagcga gcctaccctg cgcagaccgg cccaagcaac cggcaagcac
     6061 tgggcgagaa gtccggaacg cccccaccga ccctatttac tttgggaggc cgacgtggct
     6121 ataacccggc gaaagcattt ttacctacat gtggaggacc caaaaagaca tggtcaagaa
     6181 tttgctgcgc cccctgtcgg ggacgctggc actggcgacg ctgctcggcg gtgttgcgca
     6241 ggccgcatcc accgatccca ccaagcacgt cagtgaggcg acgctcgtgc tgcccgatct
     6301 cgcgtcggtg cagttcctcg gaatgtctgg caaagcgctg cttatgggcg gccttctcgt
     6361 ctgcattctc ggaatggtgt tcggtctcac catctacaac cagctcaaga acatgccggt
     6421 tcaccgctcg atgcgggaaa tcagcgagct gatctacgaa acctgcaaga cctatctgga
     6481 aacgcagggc cgcttcatcg cccttctgtg ggtcttcatc gccgcgatca tggcggtcta
     6541 cttcggcgtc ttcgaaaagc tgggcatggc caagatcatc accatcctgg tcttctcggt
     6601 cgtcggcatc ctcggtagct atggcgttgc ctggttcggc attcgggtga acacctttgc
     6661 caactcgcgc gcggcgtttg cgtcgctcgg tggcaagccg ttcccgacct acgcgattcc
     6721 gctcaaggcc ggtatgtcga ttggcatgct gctcatctcg gtcgagctgg tgctgatgct
     6781 cggcatcctg ttgtttgtgc cgcgtgacta cgccggcccc tgcttcatcg gctttgccat
     6841 cggtgaatcg ctcggtgcgg cagcgctgcg catcgccggc ggaatcttta ccaagatcgc
     6901 cgacatcgga tcggacctga tgaagatcgt cttcaagatc aaggaagacg atgcgcgaaa
     6961 ccccggcgtt atcgcggact gcacgggcga caacgcaggc gactcggttg gtccctctgc
     7021 ggacggattc gagacctacg gcgtcactgg cgttgccctt atcaccttca tcctgctcgc
     7081 ggtggcgtct gaacagaccc aggttcaact gctcgtctgg atcttcgcga tgcgcatcat
     7141 gatggtcgtc gtctcgggca tctcgtatct catcaacgag acgattgcga agggcaagta
     7201 cggcaatgcc gcgaagatgg acttcgagca cccgctgacc ttcctcgtgt ggctgacctc
     7261 gtttgtctcg gtcggcgtga cctaccttat ctcgaacctg ctcatcaaag acctgggcga
     7321 cggatcgatg tggtggaagc tgtcgacgat catcacctgc ggcacgctcg cgggcgccat
     7381 catcccggaa atgatcaagg tgtttacctc gacgcgctcg ggccacgtcc gtgaagtcgt
     7441 gacctcttcg cgggaaggcg gcgcttcgct caacgtgctg tcgggtctga cggcgggcaa
     7501 cttttcggcg tactggatgg gtctggtgtt cacgatcctg atgggcgtcg cgtactacgt
     7561 gtcgcagtcg ttcccgagcg acatgatgat cgcgccggcg atcttctcgt tcggcctcgt
     7621 cgcgttcggc ttcctgggca tgggcccggt gacgattgca gtcgactctt acggcccggt
     7681 gacggacaac gcgcagtcgg tctacgagct gtcgctgatt gagtcggtgc cgaacgtcaa
     7741 ggaagagatc aagaaggact tcggctttga gcccaacttc gagacggcca aggaatacct
     7801 cgaagagaac gacggcgctg gcaacacctt caaggcgacg gcaaagcccg ttctcatcgg
     7861 caccgctgtc gttggcgcga cgacgatgat cttctcgatc atcatgatgc tcacgaacca
     7921 cctgcaagat gcacagtcgc tgtcgaacct gacgctgctc tacccgccgt ttttgctcgg
     7981 cctcatcatg ggtggcgcgg tgatctactg gttcacgggc gcatcgacgc aagcggtgtc
     8041 gacgggtgcg taccgcgcag tggagtttat caagaagaac atcaagctcg aaggcgtgga
     8101 aaaagcgtcg gtgaccgact cgaagaaggt cgtcgagatc tgcacggtct acgcgcaaaa
     8161 gggtatgttc aacatcttcc tcacggtgtt cttctcgacg ctggcgtt
//
LOCUS       DAORGN010000214         8207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3944_length_8207_cov_5.056673, whole genome shotgun sequence.
ACCESSION   DAORGN010000214 DAORGN010000000
VERSION     DAORGN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3944_length_8207_cov_5.056673"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKL17_18180"
     CDS             complement(<1..628)
                     /locus_tag="PKL17_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46717.1"
                     /translation="MKTTIDHLPEDQQQKLHAITSVFTDPALATPIDMLILFGSRARG
                     DWVVDSETGYVSDWDLLVVVEHEKQVNDLVLWGGLEQKVRAIIGDTPLTFIVHDIKFV
                     NKEIRIGQYFFSDIANEGVLLYDSRRYMLAKPKALNAQERLALAERNFENWYDSATEF
                     WRGCRYYASRNLLKHAAFLLHQATERYYHAAILVFTGYKQRTHDIELLG"
     gene            complement(781..1317)
                     /locus_tag="PKL17_18185"
     CDS             complement(781..1317)
                     /locus_tag="PKL17_18185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46718.1"
                     /translation="MVGSRIGKYRLLKQLGEGGMGVVYEAVREDIAARAAIKVLRPEY
                     AANPELAGRFFNEARAANLIAHPGIVRVFDYGAEEGGIAYLAMEYLEGESLWKRIERQ
                     KKLPVPEALRIARQIASALAAAHEKQIVHRDLKPPSIPSRNENRTGLGKRRENRSYNH
                     PLRNGCPGFFAWDDRAGG"
     gene            complement(1440..1949)
                     /locus_tag="PKL17_18190"
     CDS             complement(1440..1949)
                     /locus_tag="PKL17_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HNK46719.1"
                     /translation="MGKFTVTHEINCDVETFWKTFFDKDFNDKLFLQTLGFPEFKVIE
                     QNDTTAKLTRKVMGQPKMDLPGPVAKLLGANFRFTEEGTWDKTSKVWTFKMIPSTLAD
                     KLRQEGTMRIESIGPTKVRRVAELFIEAKVFGVGGLIESTTEKQLRDGWDKSAVFMNR
                     YLADKAGKA"
     gene            complement(2093..4816)
                     /gene="acnA"
                     /locus_tag="PKL17_18195"
     CDS             complement(2093..4816)
                     /gene="acnA"
                     /locus_tag="PKL17_18195"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNK46720.1"
                     /translation="MTTTKDSFSAFSRLETQSGDLGIYRLSALRSLNPNFDRLPFSIR
                     VLLESLLRNCDGFVVTEEDVRRLATWDAAKPAEVELPFMPSRVILQDFTGVPVLVDIA
                     AMRAAVARLGGNPQQINPLVPVDLVIDHSVQVDHFGTKDALLRNVRLEYDRNRERYTF
                     MRWGQKSVRNFRVVPPATGIVHQVNLEYLAQGVVMRERDGVLLAFPDTVVGTDSHTTM
                     INGLGVLGWGVGGIEAEAVMLGQPLFMLTPQVIGCKLKGSLRPGVTATDLTLTITQLL
                     RKKGVVDKFVEFFGPGVSALSLFDRATIANMAPEYGATCGFFPFDGVTLDYMRKTSRP
                     ADVIDRCERYYKAQGLWRTDDMADPIFTDTVELDLSTVEPSLAGPKRPQDRVPLKSMQ
                     TSFRKALAAPVKEQGFAVSETDQGKQVATQLQSGQGAQLGHGAVLIAAITSCTNTSNA
                     SVLIAAGLVAKKAVEAGLAVAPHVKTSLAPGSRVVTEYLGRAGLLPYLEQVGFHVVGY
                     GCTTCIGNSGPIASEIEQAVTQNGLVAAAVLSGNRNFEGRIHSAIKANYLASPPLVVA
                     YALTGTVDRDLDKEPIGTGRGGQPVYLKDIWPTPEEVESIVASVITPEMFQETYGQVF
                     DGGPDWKSIAGKAEGDLYTFEESSTYIQEPPFFTTMGRTPDPVRPIVSARVLALLGDS
                     VTTDHISPAGDIPEKGPAGEYLRGHGVGKVDFNSFGARRGNDRVMVRGTFANIRLKNQ
                     LVPGVEGGVTTHLPSGERMSIFDASVRYQKDQVPLVILAGKEYGTGSSRDWAAKGTLL
                     LGVRAVIAESFERIHRSNLVGMGVLPLVFEGGQNAEKLGLTGRETYTIQTPTELLPRQ
                     TITVVATREDGSQVTFTVRSRLDTPVDVRYYQNGGILHTVLRDLLAKSAKA"
     gene            complement(5409..7343)
                     /gene="asnB"
                     /locus_tag="PKL17_18200"
     CDS             complement(5409..7343)
                     /gene="asnB"
                     /locus_tag="PKL17_18200"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNK46721.1"
                     /translation="MCGVACIVAPIPRSEREALCAEMVLALGHRGPDGQGLWSAPLDE
                     RSPDGPGITLGHRRLAIVDPTPGGAQPMQRGALVLSWNGEVYNHRALRARFKQEGAPL
                     SSDSDSEVLLTAVAKLGPVAALQEVTGPLAMLLWDGQTRTIHLARDRFGKKQLYVYRH
                     GEFLLVASEPKALVPVIHKLGLPLHVDRLTLARYLADVEQEAHDATFFAQIRRVGPAE
                     LQSFALPRRPAPIAPPTISTYYQLRPDPAPPSDQVEFQREFRQRLCLALDLRLDCDGT
                     VGALLSGGMDSSALVCLSAVLGHRLPTFSAVHRADDPCDESVFIDAVQSHTGFENFRI
                     IPETQLSPDAFARFVYEQDEPTGGASVFAQACVFRLVRSHGIKVVISGQGADEALTGY
                     GGAIPWLLADHLRHGHLTRFAQELWASKPSPGQALPALAQSGWLAFRDGLPASLQALL
                     LARKWQRSFHTTPFFRLDGLGLPDIVAGGPLADEFAARSLLHGYLYRLLSGSSLLAIL
                     RTEDRSSMAQGVESRAPFLDHRVVELCMACPPDWLVTQGRTKALLRESLSDVLPGAIA
                     NRRDKIGFGAPEARYLQGPLRPLAEEYLSKSCLGDEGLVDLPALHAAFHQGPLESRPS
                     HALWKLISVEQWLRTFGLSL"
     gene            complement(7362..>8207)
                     /locus_tag="PKL17_18205"
     CDS             complement(7362..>8207)
                     /locus_tag="PKL17_18205"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNK46722.1"
                     /translation="LTFDFSHFSPLSAKELQSVERKVNAAIRRNAAAITDECSFDDAQ
                     KRGAMALFGEKYGDRVRVLQIGESVELCGGTHVGRSGDIGLLKVTSETGIAKGIRRIE
                     ALTAEAAEEYLGSRETELLTAAETLRSGPLEVSDRVGKLLDEIKQRDREILALQKKLA
                     SASSAGLYSEEKDIGGLHVVARRVEVADGKALRELAESLVSDGKIHVIALGSAEGDKV
                     SLVVAVAKAHTGKVQAGKLVSVLAAQVGGRGGGKPEVAQAGGTDASKLDDALRSLFTH
                     VSAAP"
BASE COUNT         1510 a         2516 c         2587 g         1594 t
ORIGIN      
        1 ctcccaacag ctcgatgtcg tgggttctct gtttgtatcc cgtgaaaacc agaatcgccg
       61 cgtgatagta ccgctccgtc gcctgatgca gcaggaatgc ggcatgtttc agaagattcc
      121 ttgatgcata atatctgcat cctcgccaaa attccgtcgc gctgtcgtac cagttttcaa
      181 aattccgttc cgccagcgcg agccgttcct gggcattgag cgccttcggt ttcgcgagca
      241 tgtaccgtcg gctgtcgtac agaaggacac cttcattcgc gatgtccgaa aaaaaatatt
      301 gcccaatccg aatctctttg ttgacgaact tgatgtcgtg aacgatgaac gtgagcggcg
      361 tgtctccgat gatcgcgcgc accttctgtt ccagcccgcc ccacagcacg agatcgttta
      421 cttgcttctc atgttcgacg acaaccagca aatcccaatc agacacatag cccgtttcgg
      481 aatccacgac ccagtcccca cgcgcgcggg aaccaaacag aatcagcatg tcgatgggag
      541 tcgcaagcgc agggtctgta aagacagaag tgatcgcatg cagcttctgc tgctggtctt
      601 cgggaaggtg gtcgatcgtc gtcttcatcg ggagccttcg tgcagcgcta taccacgtcg
      661 ccaagcgctt gtcagtccgt ctctccagcc gggcccgctt tcatcactca ccgatcccac
      721 gccgatacaa caaagctaca gactctctcc gtggcgagcc ctcgcaaccg gctctccaca
      781 tcaaccaccg gcgcggtcat cccaagcaaa aaaacctgga cagccattcc taagtgggtg
      841 attgtacgag cggttttcac gccttttccc cagtcccgtt cggttttcgt ttctcgacgg
      901 aatcgagggt ggcttcaggt cgcggtggac gatctgcttt tcgtgtgcag ccgcgagagc
      961 cgaggcgatc tggcgggcga tgcgaagtgc ttcgggcacg ggcagctttt tttgccgctc
     1021 gatccgcttc cacagcgact cgccttcgag atactccatc gccagataag cgatgccacc
     1081 ttcttccgca ccgtaatcga agacgcgaac aatgcccgga tgggcgatca ggttggcggc
     1141 gcgtgcttcg ttgaagaacc gaccggccag ctcggggttc gcggcgtatt cgggacggag
     1201 aaccttgatg gcagcgcgcg cggcaatgtc ttcacgcacg gcttcgtaga cgacacccat
     1261 gccgccttcg ccgagctgtt ttaacagccg atatttaccg atgcgcgagc caaccatgtt
     1321 cgtgacggcc cgacggcagg agcgtcagca ccggctcatc aaggccaaaa caagcttgtc
     1381 gataatactc tacgctggct tttgcgcgat cggcagatcc gcttttcttg ctgtgagaat
     1441 caggctttgc cggccttgtc cgcgaggtag cggttcatga acacggcgct cttgtcccac
     1501 ccgtcacgca gctgcttttc cgtggtgctc tcgataagac cgcccacacc gaagaccttg
     1561 gcctcgatga aaagctcggc cacgcggcgc actttggtcg gtccgatgct ctcaattcgc
     1621 atcgtgcctt cctggcgcag cttgtcggcc agcgtgcttg ggatcatctt gaacgtccag
     1681 actttgctgg tcttgtccca ggtgccctct tcggtaaagc ggaagtttgc gccgagaagt
     1741 ttggcgacag gacctggcag gtccattttc ggctgaccca tgactttgcg cgtcagcttg
     1801 gcggtcgtgt cgttctgttc gatgaccttg aactcgggaa atccgagcgt ctgcaagaac
     1861 agtttgtcgt tgaagtcctt gtcaaagaag gtcttccaga aggtttcgac gtcgcagttg
     1921 atctcgtgcg tgacggtgaa tttgcccatg aatgctccct tggtagcaaa tgctacgctg
     1981 gaagcgcccg cgaatcgagg ttcaccacgt tagagacggt gaaccacaac acttgcgggg
     2041 gtagggaagc aggggactcg ctgcggcaaa gaaaagaagg ggcgggtggg cgctaggctt
     2101 ttgcgctctt ggcgagcaga tcgcgcagca cggtgtggag gatgccgccg ttctggtagt
     2161 agcgaacgtc gaccggcgtg tcgagccgtg agcgcaccgt gaaggtcacc tggctgccgt
     2221 cttcgcgggt cgcgaccacc gtgatcgtct gccgcggcag cagctccgtc ggcgtctgaa
     2281 ttgtgtacgt ctcgcgtccg gtgagcccca gcttttcggc gttctggccg ccttcaaaca
     2341 ccagtggcag cacgcccatg ccgacgaggt tcgagcggtg gatgcgctcg aagctttcgg
     2401 cgatgacggc ccgcacacca agcagcaggg tgcctttggc cgcccaatcg cgtgagctgc
     2461 cggtgccgta ttccttgccg gcgaggatga cgagcggcac ttggtctttt tggtaacgca
     2521 ccgaggcgtc aaagatggac atccgctcgc cgctgggcag gtgggttgtg acgccgcctt
     2581 cgacaccggg aaccagctgg tttttcagcc ggatgttggc aaacgtgccg cgcaccatga
     2641 cccggtcgtt gccacggcgg gcaccgaacg agttgaagtc gaccttgccg acgccgtgcc
     2701 cgcgcaggta ttcgcccgcc ggtccctttt cggggatgtc acccgcaggc gagatgtgat
     2761 cggtggtcac cgagtcgccg agcagtgcca gcacccgtgc cgagacgatc ggacggaccg
     2821 gatcgggcgt gcggcccatg gtggtaaaga acggcggttc ttggatgtac gtcgagcttt
     2881 cctcgaacgt gtacaggtcg ccttccgcct tgccggcgat cgacttccag tctggtccgc
     2941 cgtcgaacac ttggccgtag gtctcttgga acatctcggg cgtgatgacg ctggcgacga
     3001 tcgattcgac ttcttcgggc gtcggccaaa tgtccttcag gtacaccggc tggccgccac
     3061 gtcctgtgcc gatcggctct ttgtcgagat cgcggtccac ggttccggtc agcgcgtagg
     3121 ccacgaccag gggcggtgac gccaggtagt ttgccttgat cgccgagtgg atgcggcctt
     3181 caaagttgcg gttgcctgac agcactgcgg cggcgaccag gccgttctgg gtgacggctt
     3241 gctcaatttc ggacgcgatg gggcccgagt ttccgatgca ggtggtgcag ccgtagccga
     3301 cgacgtggaa gcccacttgt tccagatacg gcaaaaggcc ggcgcggccc aggtattcgg
     3361 tgacgacgcg gctgccgggg gccaagctgg tcttgacgtg gggcgcaacg gcaagtccgg
     3421 cttcgaccgc ctttttggcc accaggcccg cagcaatcag caccgatgcg ttcgacgtgt
     3481 tggtgcagct tgtgatcgcc gcaatcagca cggctccgtg gccaagctgc gcgccctgac
     3541 cgctttgaag ctgggtcgcg acttgcttgc cctggtcggt ttcgctgacg gcaaagccct
     3601 gctctttgac cggggccgcc agtgctttgc ggaagcttgt ctgcatcgac ttgagcggca
     3661 cgcgatcttg gggccgtttc ggtccggcga ggcttggctc gacagtcgaa agatccagct
     3721 cgacggtatc ggtgaagatt gggtcggcca tgtcgtcggt ccgccacagt ccctgcgctt
     3781 tgtagtagcg ctcgcagcgg tcgatcacgt ccgccgggcg gctggtcttg cgcatgtagt
     3841 ccagcgtcac gccatcgaac gggaagaaac cgcaggtggc accgtactct ggcgccatgt
     3901 tggcaatcgt tgcccgatca aacagcgaca gcgccgacac gccagggcca aagaactcga
     3961 caaacttgtc gacgacgccc tttttgcgca atagctgggt aatcgtcagc gtcaggtccg
     4021 tcgcggtgac gccgggccgc aaagacccct tgagcttgca gccgatcacc tgcggcgtca
     4081 gcataaacag cggctgaccg agcatgacgg cttccgcctc aatgccaccg acaccccagc
     4141 cgagcacgcc gagcccgttg atcatcgtcg tgtgtgagtc ggtgccaacc acggtatcgg
     4201 ggaaagcgag cagcacgccg tcccgctccc gcatgaccac gccttgcgcc aaatattcca
     4261 ggttgacctg gtggacgatg cccgtcgccg gaggaaccac gcggaagttg cgcaccgact
     4321 tttggcccca gcgcataaac gtgtagcgct cgcggtttcg gtcgtactcg agtcggacgt
     4381 tgcgcaaaag cgcgtccttt gttccgaagt gatcgacctg caccgagtgg tcgatgacca
     4441 gatcgacggg aaccagcgga ttgatctgct gcgggttgcc accgagccgc gccactgctg
     4501 cacgcatcgc cgcgatgtcg accagcacgg gcacgccggt gaagtcttgc aggatcacgc
     4561 gcgacggcat aaacggcagc tcgacttctg ccggcttggc cgcatcccac gtcgcgagcc
     4621 gccggacatc ttcttccgtg acaacgaatc cgtcgcagtt gcgcagcagc gattcgagca
     4681 gcacgcggat cgaaaacggc aggcggtcaa agttggggtt tagcgagcgc agggcggaga
     4741 ggcgatagat gccaagatcg ccagactgcg tttcgagacg agaaaaagca gagaacgagt
     4801 ctttggtggt cgtcatggca ggcatgctag caagcacacc cgcccagtgt ctacggacgg
     4861 gccgccggat gcctgcgtgc gggcgacgac cctgctgggg cgaaggatct gatccgccag
     4921 cttgtcttgt ggcgactcgt cacttcggcc caggttcctt ggcaattttg gggacgcgcg
     4981 gttcgggcgg ccagtcaggg agaggcaatc cccaaaggct gtccgcaaag gggccccgtg
     5041 ttctttcaca cgtccggggg ctggccggat ggaggcgccg cggagtgtga ccaacatgtg
     5101 ggcaggtggg gaccggggcc atggtggcgg caaaggtttc acaccgggtc cgccgcgcgg
     5161 acaagcgggc acgacaagtt ttggtcgggt tcgcagcggg gcacaccacc gtttgccatt
     5221 tctgggccgg gtctgctttg cggccagcag ggaaaatact ttttcgcggt ggttggccgc
     5281 gcggccacct gtcttcgcac gcgatggacc caatcgggcc accgtttgcg gggcggcttg
     5341 ggcttttgcg cacgaaaaag ctatgcgccc gcaggacggg gcagggtcat cggcagaagc
     5401 ggacgcgatt acaggctgag cccgaatgtg cgcagccact gctccacaga aatcagcttc
     5461 cacagcgcat ggcttggccg gctttcgagt ggtccctggt ggaatgcggc atggagcgct
     5521 ggcagatcca caagtccttc atcgccgagg caagacttcg acagatattc ttctgcaagg
     5581 ggtcgcaatg ggccttgcag gtagcgcgct tccggtgcgc caaatccaat cttgtctcgt
     5641 cggttggcaa tggccccagg cagcacgtcc gaaagacttt cgcgcagcag ggctttggtt
     5701 cgtccctgcg tgaccagcca atcgggagga caggccatac acagttccac gacacggtga
     5761 tccaaaaatg gcgcgcggga ttcgacgccc tgggccatgc tgctgcgatc ttcggtacgc
     5821 agaatcgcga gcagcgacga accagaaagc agccgataca gatagccatg cagcagtgac
     5881 cgcgctgcaa actcatcggc gagcggccca cctgccacga tgtccggcag acccagccca
     5941 tctaggcgga aaaagggcgt cgtgtggaat gaccgttgcc acttgcgggc cagcagcagc
     6001 gcttgcagcg aagcaggcaa gccgtcgcgg aacgccagcc agccagactg cgcaagtgca
     6061 ggcagcgctt gccccggcga tggtttggac gcccaaagct cttgcgcaaa gcgggtcagg
     6121 tggccgtggc gcaggtggtc agccaaaagc caagggatcg cgccgccata acccgtcaat
     6181 gcttcatcgg cgccttggcc cgaaatgacg accttgatgc cgtggctgcg gaccagtcga
     6241 aacacgcacg cttgcgcaaa gacgctggca ccgccggttg gctcgtcttg ttcgtagaca
     6301 aagcgggcaa aggcgtcggg gcttagctgg gtttcgggga tgatgcggaa gttttcgaac
     6361 ccggtgtggg actgcaccgc atcgatgaag acgctttcgt cgcaggggtc atcggcccgg
     6421 tggacggcgg aaaacgtcgg caggcggtgg cccagcactg ccgaaagaca gaccagcgca
     6481 gacgaatcca tcccgcctga cagcaaggcc ccgaccgttc catcgcagtc gagccgtagg
     6541 tccagtgcga ggcagagacg ctggcgaaac tcgcgctgga attccacctg gtccgatggt
     6601 ggcgccgggt cggggcgcag ctggtagtac gtcgagatcg ttggcggtgc aatcggggcg
     6661 ggccgcctgg gcagcgcaaa cgactgaagc tcggcggggc cgacgcgccg aatctgcgcg
     6721 aaaaacgtcg cgtcatgggc ctcttgttcg acatcggcca gataacgcgc cagcgtcagc
     6781 ctatcgacgt gaagcggcag tcccagcttg tggatgacgg ggaccagggc tttcggctcg
     6841 gacgccacca gcagaaactc accgtgtcga tagacataga gctgcttttt gccaaagcgg
     6901 tcgcgcgcca ggtggattgt gcgggtctgt ccgtcccaca gcagcatggc gagcggcccc
     6961 gtcacctctt gcagcgccgc caccggaccc agcttggcca ccgccgtcag aaggacttcg
     7021 ctgtcggaat ccgaagagag cggggcgcct tcctgtttga agcgggctcg cagggcacgg
     7081 tgattgtaga cctcgccgtt ccacgaaagc acaagggcac cgcgctgcat cggctgggcc
     7141 ccaccgggag tcggatcgac aatcgcgagc cgccggtgcc ccagcgtaat ccctggtccg
     7201 tccgggctgc gttcatcgag cggcgcgctc catagacctt ggccatcggg cccgcgatgg
     7261 cccagtgcca aaaccatttc ggcgcacagg gcttcgcgct cgctgcgagg gatcggcgcg
     7321 acgatgcagg cgacgccaca catgctgtga ctatacggga attagggcgc agcggagacg
     7381 tgggtaaaca gcgaacggag tgcgtcgtcg agcttgcttg cgtcggtgcc gccggcttgg
     7441 gcgacttccg gcttgccgcc cccgcgaccg ccgacttgcg cggccagcac cgacaccagc
     7501 ttcccggctt ggacttttcc ggtgtgcgcc ttggcgaccg cgaccaccag cgacactttg
     7561 tcgccttcgg cactgccgag cgcgatgacg tggattttgc catccgaaac cagcgattcg
     7621 gccagttccc gcagggcctt gccatcggcg acttcgaccc ggcgcgcgac cacatgcagt
     7681 ccaccgatgt ctttttcttc cgaatacagg cccgcactcg atgccgaagc gagcttcttc
     7741 tgcagggcca ggatttcacg gtcacgctgc ttgatttcgt cgagcagctt gccgacccga
     7801 tcgctcactt cgagcggacc cgatcgcagc gtttcggcag cggtgaggag ttcagtttcc
     7861 cggctgccca ggtactcttc ggcggcttcg gcggtgagcg cttcgatccg acggatgccc
     7921 ttggcgatgc cggtttcgga cgtgactttc agcaggccga tgtcaccgct gcggcctacg
     7981 tgcgtaccac cgcatagctc gacggactcg ccaatctgca aaacgcggac gcggtcgccg
     8041 tacttttcgc caaacagcgc catggcaccg cgcttttgcg catcgtcaaa gctgcattca
     8101 tcggtgatgg ccgcggcgtt gcgacggatg gcggcgttga ctttgcgctc gacgctttgc
     8161 agttctttgg ccgacagcgg cgaaaagtgc gaaaagtcga aggtcag
//
LOCUS       DAORGN010000215         8168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3964_length_8168_cov_5.414643, whole genome shotgun sequence.
ACCESSION   DAORGN010000215 DAORGN010000000
VERSION     DAORGN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_8168_cov_5.414643"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKL17_18210"
     CDS             complement(<1..188)
                     /locus_tag="PKL17_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46723.1"
                     /translation="MRSRLLPRWFLPPLLGISLALLAGCNRGPKPAPPKTPSAITTAG
                     PSALTSPDGDLRACCKQC"
     gene            complement(194..1435)
                     /locus_tag="PKL17_18215"
     CDS             complement(194..1435)
                     /locus_tag="PKL17_18215"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNK46724.1"
                     /translation="MSTEQAMLPWKKARELILGLSTPLGPETVELSAASGRILTTDIV
                     STRQLPPWDNSAMDGYAVRAADVTVGAKLPVSGTIAAGHPAETPLVAGTAMRIMTGAP
                     MPALADAVIMREEADEADGQVSFRKAPRPGQHVRRAGEDVQPGSAVLLAGALLSAGEI
                     GILAALGRLLVSVHRRPSVAIVSTGDELVRADEQPHSGQIVNSNAHCLLALVEQAGCR
                     GRILPIARDTKEDLQRCFAEAFSADVVISSGGVSVGDFDFVKQVFAELGAKERFFRVA
                     MKPGKPLVFSTVEQHGRLTLLFGLPGNPASSLVSFELFVYPALRRLLGHPVAQAAHPL
                     AMVRIAEPIRPDRERTHFTRALVTRSAVPPYELWAKPLTQQGSGMLRSLVGMNALLEV
                     PPGEASLPAGSELCANLLSVV"
     gene            complement(1447..2691)
                     /locus_tag="PKL17_18220"
     CDS             complement(1447..2691)
                     /locus_tag="PKL17_18220"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46725.1"
                     /translation="MKTVRNCRPAASLLLSLAIAACTPAAESPIPDPTMDPVPPDGSP
                     TYYRDVKPVIVKRCQGCHVPGSIGPFPLVTYDDAKGKAALLAESVLAKRMPPWKPGPD
                     CQQFAPDRRLLDSEIALFQAWAKAGAPEGNKADDKTTAPTVLSLPNPSATLDWGAAYT
                     PDATKSDDYHCLLIDPKLTQDQDLVAYEFAPDQRHEVHHALIYSAPMADAKAKEDATP
                     GLGWPCSGSSDVKSAQLVATWVPGETYSEYPAGTGIRIPAGHALIAQMHYNLRHGSAP
                     DRSQLKLRYADRPVPNRASIVPILNTSFTINPHQQGATAVATSGALPVALKVWGVFPH
                     MHELGRSGKVVAGNNCLIDIPKWDFHWQQMFFYAGGGLTVPKGTEVKYTCTWDNPTDR
                     TVRWGEGTEDEMCIAFFYTTLH"
     gene            complement(2738..3565)
                     /locus_tag="PKL17_18225"
     CDS             complement(2738..3565)
                     /locus_tag="PKL17_18225"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNK46726.1"
                     /translation="MMPVQIRITADDFGWTRAQNQAVEQACAAGTLTHASLLTNGFAV
                     DDAVAVAKRVPQLGVGVHLTLCEGAPLSDPAALAGLTSADGHFHDGLSPLLSLYLRRS
                     LPLSAIETEWQTQIDRALSLGFSLTHIDGHKHVHVLPPLIEIACRLAQRYRIPFVRSP
                     YESPSSRIIRRLPGWLVLSSLALSARQVILRHGLRTADHFVGFSVSGGMTEAYLLAAI
                     AAAPSGLTEIMVHPAEESEDLAPLRARYDWAHSYRFGGELAALCSPQVRAALLRRAA"
     gene            3700..4305
                     /locus_tag="PKL17_18230"
     CDS             3700..4305
                     /locus_tag="PKL17_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNK46727.1"
                     /translation="MAHTPKNYDQLIAECAGFLSENQLKAHFALYQGYVKKLNEIEER
                     LGKADPTTANYSFSDYSELRRREPVAYNGTILHELYFQGLGAAGQTPSDAFKKAVESS
                     FGSWDAWIADIKGIVGSGHGWCLVTYDYNFHKVRNSQVQGEHHIGLQANQWILAAIDA
                     WEHAYFLDYGTKKPDYLAGVLKHLNWSVISGRLPDVASKKA"
     gene            complement(4452..5711)
                     /locus_tag="PKL17_18235"
     CDS             complement(4452..5711)
                     /locus_tag="PKL17_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46728.1"
                     /translation="MKEIARRVASPARQKSTSSKLLLAGVAVSILAAAGGCTSLLSKL
                     SLRAAYKPKPHKTVQMQGVYRLWAGDMNFHISPPDDPRKIRRTLKESVDLVNASGLDF
                     VFVTPQIPARFWESADQFERVKRDWPSLQATLSSLSEKGPLMMVGAEYVDDKNGSATI
                     IGTDIPKALADISRDDLRTNPTAFVNLLWMRGALVFLNTPLATPVKAPVDSHQSYSST
                     DRSWKPLTKPSAGFPKDILALNNIYDGMEAYSVPLSVWRDQYALGDPVASVAAVMSRL
                     DLEIIRKKRRLIPIGGTDSRGSLIRPTVYIAAPERTPEALRQGLTNGRVCVRSPTPCG
                     VRVFADDQPVAAGVGDHIVASQHIDIRWEGGEGELFRNAESIGTFEGRALQPTSRSEC
                     HIYRLVVDGGYSAPFFVNCPALAPARP"
     gene            complement(5827..7335)
                     /locus_tag="PKL17_18240"
     CDS             complement(5827..7335)
                     /locus_tag="PKL17_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNK46729.1"
                     /translation="MMGNDRQDPDRAIRRAGDSPRIPGNTVTLLRDGPAVFASWLSDI
                     AKAKRFILLENYIFRSDRIGRQIADALCERARAGVEVYVLYDWLGSLGTSRTLWVRLQ
                     REGVRVRSFAGIRFSDPLRLLQRNHRKVLCIDGQIGHVGGLCIGDAWAGHPERGLAPW
                     RDTAVRFEGPAAAELCLAFDETWGVAGSALPSRIRIPDDVLAPQPILTPVADPQEDTP
                     CCGAPIRVVSGLPGRSRIYRLTQVLLANAASRIWITDAYFLTPTTMYEALTAAARDGV
                     DVRVLVPGRSDLPWISWASRAGYVGLLEAGVRIFEWQGPMLHAKTTVVDGQWCRVGSS
                     NLNLASLLTNWELDVVIEDATFGHAAEQMFLRDLSESRELHLRSRKSRNASLGQGESA
                     EALPATSVALAKNPPAPPPVMVSGRAGAAVVRASAAVIGVALRRRYERSAWSISIVAA
                     LVLLSFGGIGFVVPQLFGFLIAGFCVWFGIGSLFRGISDFRHHARRKTRRPR"
     gene            complement(7389..>8168)
                     /locus_tag="PKL17_18245"
     CDS             complement(7389..>8168)
                     /locus_tag="PKL17_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNK46730.1"
                     /translation="RKGKEMVEMRDFEEAKDKVLMGPERKSMVISEKERHITAIHEAG
                     HTLVASMLPGTDPVHRVTIIPRGPALGVTQQLPTEDRLNATKEWAESKIAVLMGGRVA
                     EELVFSHLTTGAGSDLEHATDLARKMVCNWGMSDKLGPLTFGRKEEHVFLGREIGQTQ
                     NYSDDTARIIDDEVKRIVMEQYGRAKDLLTAHQAELQRLADSLLTHETLSGEDVDLVL
                     KGGTLPERPPRGLKSTRSEPTPAKTQTPPLLGPSVLTPEKA"
BASE COUNT         1556 a         2479 c         2627 g         1506 t
ORIGIN      
        1 atacactgct tgcagcaggc gcgcagatcg ccatcaggcg atgtcaaagc cgaaggacca
       61 gccgtcgtga tggccgatgg ggttttgggt ggcgcgggtt ttgggccccg gttgcagcca
      121 gcaagtagcg ccagggaaat cccgagtagc ggtggcaaaa accaacgtgg cagaagacgg
      181 gaacgcatgc ggactagacc accgaaagca ggttggcaca cagctcgctg cccgctggta
      241 gtgatgcttc accgggaggg acttcgagca gcgcattcat cccaaccaac gatcgcagca
      301 tccccgaccc ctgctgggtc agcggtttgg cccacagctc atacggtgga accgcggatc
      361 gcgtgacgag tgcgcgggtg aagtgggtgc gctcacggtc cggtcggatc ggttcggcaa
      421 tccgcaccat cgccagtgga tgggccgctt gtgcgaccgg atgaccaagc agccttcgca
      481 gggccggata gacgaacagc tcgaagctca ccagggacga cgcgggattg cctggcagcc
      541 caaacaaaag ggtcagtcgt ccatgctgct caaccgttga aaacaccagc ggcttgcccg
      601 gcttcattgc cacacggaaa aatcgttcct tggccccgag ctccgcaaag acctgcttta
      661 cgaagtcaaa gtcacccacg gatacaccgc cggacgagat gacgacatcg gcggaaaatg
      721 cttcggcaaa gcagcgctgc aggtcttctt tggtgtcgcg ggcaatcggc aaaatgcgtc
      781 cgcggcagcc ggcttgctcg accagtgcga gcaagcagtg ggcgttgctg ttgacaatct
      841 gaccgctgtg cggctgctcg tccgcgcgga ccagctcatc gcctgtcgat acaatggcga
      901 cgctcgggcg gcggtgaaca ctgaccagca gtcgcccgag cgcggccaag atgccgattt
      961 cccccgcgga cagaagcgca cctgccaaaa gcacggcgct gcccggctgg acatcttccc
     1021 cggcccgacg gacgtgctgg ccggggcgcg gtgctttgcg gaaagacacc tggccgtctg
     1081 cctcgtcggc ttcttcgcgc atgatgaccg catcggccag ggcgggcatg ggagcgcccg
     1141 tcatgatccg catcgccgtg cctgccacaa gcggtgtttc tgccggatga ccggctgcaa
     1201 tcgttccgct caccggcagc ttggcaccca cggtgacatc ggcggcccgc accgcgtagc
     1261 catccatggc cgagttgtcc cacggcggga gctgccgggt cgaaacgatg tcggtggtga
     1321 gaatccgccc actggccgca gacagctcga ccgtctcggg gccaagcggc gtgctaagcc
     1381 ccaagataag ctcgcgcgct tttttccagg gcagcatggc ttgctcagtg ctcatcgggg
     1441 cggcctctag tgcaacgtgg tgtagaagaa cgcgatgcac atctcgtctt cggtgccttc
     1501 accccagcga accgtgcgat ccgtcggatt gtcccatgtg caggtgtatt tgacctcggt
     1561 gcctttgggg acggtgagcc cgccgcctgc gtaaaagaac atctgctgcc agtgaaagtc
     1621 ccatttcgga atgtcgatga ggcagttgtt cccggccacg accttgcccg accgcccaag
     1681 ctcatgcatg tgcgggaaga cgccccaaac tttcagtgca accggcagcg cgcctgatgt
     1741 cgcgaccgca gtggcgccct gctggtgcgg attgatggtg aagctggtgt tcagaatcgg
     1801 gacgatcgaa gcgcggtttg gcaccgggcg atcggcatag cgcagcttga gctggctgcg
     1861 atcgggggct gatccatggc gcaggttgta gtgcatctgc gcaatcagcg cgtggcccgc
     1921 ggggatgcga atgccggtgc cggctgggta ctcgctgtag gtttcgccgg gaacccaggt
     1981 cgcgacaagc tgcgccgatt tcacgtccga gctgccgctg catggccagc caagtcctgg
     2041 tgtcgcatct tctttggctt tggcgtccgc catcggtgcc gaatagatga gcgcatggtg
     2101 gacttcgtgc cgctggtcgg gcgcaaactc gtaggcgacc aagtcttgat cttgggtcag
     2161 cttcggatcg atgagcaggc agtggtaatc gtcgctcttg gttgcatccg gggtatatgc
     2221 cgccccccaa tcgagcgtgg ccgatggatt gggcagcgat agcacggtcg gcgcggtcgt
     2281 cttgtcatcg gccttgtttc cttcgggcgc gccggctttg gcccaggctt ggaacagcgc
     2341 gatttccgaa tcgagaagcc gacgatcggg tgcaaactgc tgacagtccg gtcccggctt
     2401 ccacggcggc attcttttgg ccagcaccga ctctgcgagc aatgccgctt tgcccttggc
     2461 atcgtcgtag gtcacaagcg gaaacggtcc gatgctgccg ggcacatggc agccctggca
     2521 gcgcttcacg atgaccggct ttacatcgcg ataataggtt ggtgatccat cgggtggaac
     2581 cgggtccatc gtcggatcgg gaatcggact ttcagcagcg ggtgtgcagg cggcgatcgc
     2641 cagcgacaaa agcagcgagg cagcgggacg acaatttcta acagtcttca tgtccgatcc
     2701 tttccatcgt agggtggcgg ggacgttggg cgattggtta ggccgctctg cgcagaaggg
     2761 ctgcacgcac ctgcggactg cacagcgccg cgagctcacc gccaaagcga tagctgtggg
     2821 cccagtcgta gcgggcgcgc agcggtgcga ggtcttcgga ttcttctgcc gggtggacca
     2881 tgatttcggt aagtccagac ggggcagcgg cgattgcggc cagaagatag gcttcggtca
     2941 tgccgccact tacggaaaaa ccgacaaagt ggtcagcggt cctaagccca tggcgcaaga
     3001 tcacctgccg tgcagacagc gccaaactgg acagcacaag ccaaccaggc agccggcgga
     3061 tgatccggct cgacggcgat tcatagggcg agcggacaaa ggggatgcga tagcgctgtg
     3121 ccagacgaca cgcgatttca atcagcggcg gcagcacatg gacatgcttg tgcccgtcga
     3181 tgtgggtgag agaaaaaccc agcgacagcg cgcgatcgat ctgggtctgc cactcggttt
     3241 caatcgccga aaggggcagc gaccgacgca ggtacaaaga caaaagcggt gaaagaccat
     3301 cgtggaagtg accgtctgcg gacgtcagtc cggccagggc cgctgggtcc gatagcggtg
     3361 ccccttcaca cagcgtcagg tgaacgccga cgccaagctg gggaactcgc ttggccacag
     3421 ccacggcatc atcgacggca aagccatttg tcaaaagcga ggcatgcgtg agcgttccgg
     3481 cggcacaagc ctgctctacc gcttggtttt gtgcgcgggt ccagccgaaa tcatcggcgg
     3541 tgatgcgaat ttgaaccggc atcatgcagc gggaacttgg cacgatgggc ttctctgtcg
     3601 caagcgcacg agcaccaaaa ttgctcgctg acctagggta agttatggca tctaaccaaa
     3661 cccatagacc actacccacg gtagagagga actgcaaaga tggctcatac ccccaagaac
     3721 tacgatcagc tcatcgccga gtgcgcgggc ttcctgtcgg aaaaccagct caaggcgcac
     3781 tttgcgctgt accagggtta cgtaaagaag ctaaacgaga tcgaggagcg gcttggcaag
     3841 gccgacccga cgacggcaaa ctactcgttt tccgactact cggagctgcg gcggcgggag
     3901 ccggtggcct ataacggcac catcctgcac gagctgtact tccagggact tggcgcagcg
     3961 ggccagaccc catcggatgc cttcaagaag gcagtcgagt cgagcttcgg ttcgtgggac
     4021 gcctggattg ccgacatcaa gggcatcgtc ggttcgggtc acggctggtg ccttgtgacc
     4081 tacgactaca acttccacaa ggtccggaac agccaggttc agggcgaaca ccacatcggc
     4141 ctgcaagcga accagtggat tttggcggcg atcgacgcct gggagcacgc ctacttcctg
     4201 gattacggca ccaagaagcc cgactacctg gccggtgttc tcaaacacct gaactggtcg
     4261 gtcatcagtg gccgcctgcc agacgtcgcc agcaagaagg cgtaatcaag tccgcctgca
     4321 tcctgtcttt ttacgccgcc agattccttc tggcggtgcc ttcggatgtt ctttttccac
     4381 cgcagacaaa agcccggccc gtcccgatcg cttacgcgag gggatggacc gatgggttct
     4441 tttggcgtgg attacggtcg ggcaggcgcc agggcagggc agttgacgaa gaacggagcg
     4501 gagtagccgc cgtcgacgac caagcggtag atgtgacatt ccgatcggct ggtcggttgc
     4561 agcgcgcggc cttcaaaggt tccgatgctt tcggcattgc ggaacagctc gccttcgccg
     4621 ccttcccagc ggatgtcgat gtgctgactg gcgacgatgt gatcgccgac gcccgcagcg
     4681 acgggctgat catcggcaaa aacccgcacg ccgcacggcg tgggcgaccg aacgcagacg
     4741 cgaccgttgg tcaatccttg ccgcagggct tcgggcgttc gctctggtgc cgcgatgtag
     4801 accgtcggac ggatcagcga accacggctg tcggtgccac caatcgggat gagccggcgc
     4861 tttttgcgaa tgatttccag atcgagacgg ctcatcaccg ccgccaccga agccaccgga
     4921 tcgccgagcg catactgatc gcgccacacc gaaagcggca ccgaataggc ttccatgccg
     4981 tcgtagatgt tgtttagcgc gaggatgtct ttggggaaac ccgccgatgg cttggtcagc
     5041 ggcttccagc tccgatcggt gctcgagtag ctctggtgcg aatcgaccgg ggctttcacg
     5101 ggcgtcgcca gcggggtgtt gaggaacacc agggcgccac gcatccacag caagttcaca
     5161 aatgccgtcg ggttggtccg caggtcatcg cgcgagatgt cagccagcgc cttggggata
     5221 tccgttccga tgatcgtcgc cgaaccgttt ttgtcgtcga catactcggc accgaccatc
     5281 atcagcgggc ctttttcgga cagcgatgac agcgtcgctt gcaacgaagg ccagtcgcgc
     5341 ttgacccgct cgaactggtc agcactttcc cagaagcgcg ccgggatctg gggtgtcacg
     5401 aagacgaaat cgagccccga agcgttcacc agatcgaccg actctttgag cgtgcgcctg
     5461 atcttgcgcg ggtcgtccgg cgggctgatg tggaagttca tgtcgccggc ccacaggcgg
     5521 tagacgccct gcatctgcac cgtcttgtgc ggcttgggct tatacgctgc tctgagcgac
     5581 agcttcgaca gcagcgaggt gcaaccaccc gctgcggcca gaatcgatac cgcaacaccg
     5641 gccaagagca gctttgaaga ggtgcttttt tgcctggccg gggatgccac gcgccgtgcg
     5701 atttctttca tcagcgcgat agtagggggg ttttgccgtc aaagtcaaag gtcgtgtgcg
     5761 acagcgaatt gcggcctttg cgcggacatc tgggcggtgg cgtgtttggg gcgggggtcg
     5821 attgtttcag cggggacggc gggtcttgcg gcgcgcgtgg tggcgaaagt cggaaatccc
     5881 gcggaaaagc gagccgatgc caaaccacac gcagaaccct gcgatcaaaa agccgaacag
     5941 ctgcgggacg acaaagccga tcccgccaaa tgacaaaaga accagcgccg cgacaatcga
     6001 aatgctccag gccgatcgct cgtagcgtct gcgcagggcc acaccgatga ccgcggcact
     6061 ggcacgaacc acggcggctc cggcgcgacc ggacaccatc acgggcggcg gggcgggcgg
     6121 atttttggcg agcgcgacac tcgtggccgg cagtgcttcg gcgctttcgc cttggcccag
     6181 gctggcgttg cggctcttgc ggctgcgcag gtgcagctcg cggctttcgg acaggtcgcg
     6241 caaaaacatc tgctcggcgg catgtccgaa agtggcgtct tcgatgacga cgtcgagttc
     6301 ccagttggtg agcaggctgg ccaggttcag gttggacgaa ccaacgcggc accactgccc
     6361 atcgaccacc gtggttttgg cgtgcagcat ggggccttgc cactcgaaga tccgcacgcc
     6421 ggcttcgagc agaccaacgt aaccggcccg gctggcccag gatatccacg gcaggtccga
     6481 tcggcccggc accagcacgc gaacatccac gccatcgcgc gccgctgccg tcagcgcttc
     6541 gtacatcgtc gttggcgtca ggaagtaggc atcggtgatc cagatccgcg acgccgcatt
     6601 ggccaacaga acctgggtca gtcggtagat ccgcgatcgg cccggcagcc cggacaccac
     6661 gcgaatgggg gcgccacagc aaggcgtgtc ttcttgcgga tcggcgaccg gcgtgaggat
     6721 gggctgcggg gccagcacat cgtccggaat gcgaattcga ctcggcagcg ccgatccggc
     6781 cacgccccag gtttcatcaa aggccagaca cagctcggcg gcagccggtc cttcgaaacg
     6841 aaccgcagtg tcgcgccacg gggcgagccc gcgctcggga tgtccggccc aggcatcccc
     6901 aatgcacaag ccgccgacgt gtccgatttg gccgtcgata caaagcacct tgcggtggtt
     6961 tcgctgcaga agccgcagcg gatcggaaaa gcgaatgcca gcaaacgatc ggacgcggac
     7021 gccctcgcgc tgaagacgaa cccacaaagt tcggctggtg cccaagctgc ccagccagtc
     7081 gtacagaacg tagacttcga cgccggccct ggctctttcg catagcgcat cggcgatctg
     7141 gcggccaatt cggtccgatc ggaaaatgta attttcgagc aggatgaagc gcttggcttt
     7201 ggcgatgtcg gaaagccagg acgcaaagac agcggggccg tcgcgcagaa gcgttacggt
     7261 gttgccgggg atgcgggggg aatcgcccgc gcggcgaatc gcgcggtctg gatcttggcg
     7321 gtcattcccc atcatggcgt tgagcactca ggttgtctcc tcgccgcggg tcgaccgagg
     7381 gaggcaggtt aggctttttc gggtgtcaga acagacgggc caagaagtgg tggcgtctgc
     7441 gtcttggccg gggttggctc gctgcgggtg gacttgagcc cgcgcggcgg acgttctggc
     7501 agcgttccgc cttttagcac cagatcgaca tcctcaccgc tcagcgtttc gtgggtgagc
     7561 agtgaatcag cgagcctttg cagctcggcc tggtgggcgg tcagaagatc cttggcccgc
     7621 ccatactgtt ccatgacgat gcgcttgact tcgtcgtcga tgatccgcgc cgtatcgtca
     7681 gagtagttct gggtttggcc aatctcgcgt cccaaaaaga cgtgctcttc tttgcgcccg
     7741 aacgtcagcg gccccagctt gtcgctcatg ccccagttgc agaccatctt gcgcgccaaa
     7801 tcggtggcat gttcgagatc gctgccagcg ccggtcgtca gatgcgaaaa gaccagctct
     7861 tccgcgaccc gtccgcccat cagaacggcg attttgcttt ccgcccactc cttggtcgcg
     7921 ttgagccgat cttccgtcgg gagctgctgg gtcacgccga gcgccggacc acgcgggatg
     7981 atggtcacgc ggtggacggg gtctgtcccc ggcagcatgc ttgcgaccag ggtgtggccc
     8041 gcttcgtgaa tcgcggtgat gtggcgctct ttttccgaaa tgaccatcga cttgcgctcg
     8101 gggcccatca ggactttgtc cttggcttcc tcgaagtcgc gcatctcgac catctctttg
     8161 cctttgcg
//
LOCUS       DAORGN010000216         8152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_3986_length_8152_cov_5.218476, whole genome shotgun sequence.
ACCESSION   DAORGN010000216 DAORGN010000000
VERSION     DAORGN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_8152_cov_5.218476"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PKL17_18250"
     CDS             complement(<1..1204)
                     /locus_tag="PKL17_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46731.1"
                     /translation="MRQSHKTLLVWLMMGLASVAAYQLISSARLSEEKKPSFSDFMQE
                     VEKNPAHIKSVKIDGQEFFVSYNESPPNDRARIVGPSELAHSQVLAALDKSKIRYEIE
                     KPYEGGLLTLILSTVLPMVLVVLLFFLMMRQLQAGGGKAMSFGKAKARLLTENQNKVT
                     FSDVAGVEEAKDEVEEIIAFLKDPKKFTRLGGRIPKGVLMMGPPGTGKTLLARAIAGE
                     AGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKRHAPCIIFIDEIDAVGRHRGAGLGG
                     GHDEREQTLNQLLVEMDGFESNDGVIMIAATNRPDVLDPALLRPGRFDRRIVVPRPDV
                     KGREGILRVHARRVPLGPDVDLAVVSRGTPGFSGADLESLVNEAALMAARKGKEMVEM
                     RDFEEAKDK"
     gene            complement(1372..2865)
                     /gene="tilS"
                     /locus_tag="PKL17_18255"
     CDS             complement(1372..2865)
                     /gene="tilS"
                     /locus_tag="PKL17_18255"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNK46732.1"
                     /translation="MGTPPPNTVPCASTQTAQLLQLAHKLRQSPVVTHCEKALLGAGI
                     RPGARLVLGCSGGPDSTALMVALWLLSPRHHWHVQVVSVDHGLRKEAAREAQQVVAVA
                     TQLGLSAQSVRVAVPRTASIQSAARTARYEALESVARKTHAQAVLTAHTLDDQAETVL
                     LRLTGGAGLRGLGGIPKARPLSQSLPGCLLLRPLLAVEKTQVLALIDQAASVLAPLPL
                     HDPSNDNRSFRRVAVRHELLPALSAIAPAQKTHLAQLADQLRADAELLDQLAATQLPG
                     VLAVEVPKLPGQLAALSTTKLAALPRPLALRVLQQVIGRPLASVHWQAILQLASSQHG
                     SQRVDLPHGLFAERRYETLYLRRHQPDTAPIIDSAPERAIACAGEYSIGLGRVRLRVL
                     APNEHPAVDGKSQVLIRLVAPAFPLGLRRVRPGDRLRLPAGHRKVSDLWTDLKLPKDE
                     RAKLWVLTKNDQPLWLLGVRAGLCEDFDPALPQLQLFGEFVAEPLPP"
     gene            2893..4071
                     /locus_tag="PKL17_18260"
     CDS             2893..4071
                     /locus_tag="PKL17_18260"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNK46733.1"
                     /translation="MIHLVVMKVRACRLKTCAVGTLLAGLAVASPLWAGRGAAGPVAS
                     SVATPDLPSRESLDVVKKRASEQRKMLVIELGAAWCTPCKQFESEVLPKESVRVALGQ
                     VYFVRYDAETEVGKQAARALSVEGYPTFLALRTDGEVASIVQGFQSETEFVHWISQVA
                     PDFEPSAMLDERLKKNPADGEAMLLVALRQRKAGNLQAALGWLEKARVAKTASADSQA
                     KADWELRRLRLRLLLPRQQLVEHLDQFPSGPHKDEALRALLRLGPLDAVAQGAVARYL
                     DRALPTLAPDRLNQLVYRLLRAQAFPEAERVTRHLLSIDPRSPYYLDTLAEIFHLRGD
                     SKEALRLSGQALGQVKGASGEALRQSLLQNQARFARARKEPPAELATPDDELQPWEKD
                     "
     gene            complement(4072..4353)
                     /locus_tag="PKL17_18265"
     CDS             complement(4072..4353)
                     /locus_tag="PKL17_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46734.1"
                     /translation="MVNVTARKIREHGRDAREKPSACAGRVYHGLLVSYPTVGRGMVI
                     FRDPNGHRMVTTPVRRVLTDPDSDSVYVETENSVYRLTFHAAIPLAAAC"
     gene            4463..5350
                     /locus_tag="PKL17_18270"
     CDS             4463..5350
                     /locus_tag="PKL17_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK46735.1"
                     /translation="MSDMQSARPDLAQVVPQAPWIRRIPLRSPTLPPATHTNAYVIGE
                     SDLVIVDPGTPWPDAQAPLWDALRGLQSAGLVPRAIVLTHHHLDHASAAAELSREFSL
                     PVWAHPQTTERLSGRIEVEIARSLHDGELLPFGPSGLRVLHTPGHAPGHVCLLDEAGG
                     GLIAGDMVASQGTIIVSPRDDGDMALYLASLRRLLSLGPLRVWPAHGVAVEEGDKLLG
                     RYLDHRLLREEKVVRALSSPAHTIDALLPLAYDDTPEALYPLARESLLAHLLALVSSG
                     RVARRPQPSGEDHFVLLHP"
     gene            5492..6568
                     /gene="fni"
                     /locus_tag="PKL17_18275"
     CDS             5492..6568
                     /gene="fni"
                     /locus_tag="PKL17_18275"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNK46736.1"
                     /translation="MSQQDPERAQLPLISQRKSEHIALCASGDVEFRQKGTLLDQVQF
                     VHQAIPERHYDEIDLSTPLLGKVLKAPIVLSAMTGGTDEAQQINHDLARAAETLGLGF
                     GLGSQRAMLVRPETTRTFQVREHAETALVLGNIGLVQAARMSTAEVRSLCKTVSVDAL
                     CVHLATGMELVQPGGDRDFRGGLDTLRRLHGELGIPIVVKETGCGLSRQVGLMVREIG
                     ITAVDVSGAGGTSWVGVETHRAEGVARAVGEALWDWGVPTAASVSLLSDLNLQIIATG
                     GLRSGLDVAAAIALGARAGGLAAPVLKAWKAGGYDGAVEYLRGVIETVKAVTFLTGCR
                     TPAELASAPRVIGSTLRAWQEGAR"
     gene            6573..>8152
                     /gene="mvk"
                     /locus_tag="PKL17_18280"
     CDS             6573..>8152
                     /gene="mvk"
                     /locus_tag="PKL17_18280"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNK46737.1"
                     /translation="MLKTSACGKVILLGEHAVVYGVPALCGALQGGVEVEVIPGQGRL
                     IVPEWEVATPMLATLLDPTTRHGHSSLADALVAIVESSFAAVSRPLSAADLPVDFVAR
                     FAIPTGAGLGSSAALSVALVRAVDRAAELGLSESDIDAAAFAAERVFHGSPSGLDHTV
                     AQRGGFGLYRRGHGLSVLTGTPALRLCIGHTGRARDTKGRVARVAELTKQQPDKTAAI
                     FARIATLVEESAAALARHDFARLGDAMNENQQLLSLLDVSCPEIEQVCAIARNAGALG
                     AKLTGGGGGGCVVALAPGCEDAVLAAWQAAGYSAFLVEVGGAGQTAKAAGPAAKTGSV
                     SEQAMHAVASANTNIALVKYWGKCDPKLNLPAVPSLSLTLAGLTTHTEVTLDPGRQAD
                     ELVLNDKSVSGEPLRKVSRHLDRLTRHLGLAGRPFALVRSHNNFPTAAGLASSASAFA
                     ALTVAGYGALLGEKSLSTPLARSVLSSLARQGSGSAARSLFGGLAVLGVGTPGQVDSA
                     LASQLLTPEEWPDLRLVIG"
BASE COUNT         1408 a         2442 c         2784 g         1518 t
ORIGIN      
        1 ctttgtcctt ggcttcctcg aagtcgcgca tctcgaccat ctctttgcct ttgcgggcgg
       61 ccatcagcgc cgcctcgttc accagcgatt ccaggtcggc acccgaaaag cccggcgttc
      121 cgcgcgaaac caccgccaga tcgacatctg gaccaagcgg cacccggcgg gcatggacgc
      181 ggagaatgcc ttcgcggcct ttgacgtcgg gacgcggcac cacaatgcgg cgatcgaagc
      241 ggcccggacg aagcagcgcc ggatcaagaa cgtcggggcg gttcgtcgcg gcgatcatga
      301 tcacgccgtc gtttgattca aagccgtcca tctcgaccaa aagctggttg agcgtctgct
      361 cgcgctcgtc gtgaccgcca cccaggccgg cgccacggtg acgaccgacg gcatcaattt
      421 catcaatgaa gatgatgcag ggggcgtgac gcttgccctg ctcgaacagg tcgcgcaccc
      481 ggctggcacc gacgcccacg aacatttcga cgaaatcgct gcccgaaatg ctgaagaacg
      541 gcacgcccgc ttcaccggca atggcccgcg ccagcagcgt tttgccggtc cccggcgggc
      601 ccatcatgag gacgcctttg ggaattcggc cacccaggcg ggtgaacttt ttggggtctt
      661 tgaggaaggc gatgatttct tcgacttcgt ctttggcttc ttcgacgcca gcgacatcgg
      721 aaaatgtcac tttgttctga ttttccgtca gaagtcgcgc cttggctttg ccaaagctca
      781 tcgctttgcc gccgccggct tgaagctgcc gcatcatcag gaagaacagc agcacgacga
      841 gcaccatcgg caaaactgtc gacaggatca gcgtgaggag accgccctcg tagggttttt
      901 caatctcgta gcggatcttg gacttgtcga gcgcggcaag tacttggctg tgggccagct
      961 cggacggccc cacgatgcgc gcccgatcgt ttgggggcga ctcgttgtag cttacgaaaa
     1021 actcttggcc gtcgattttg acggacttga tgtgggcggg gttcttctcg acctcttgca
     1081 taaagtcaga gaagctcggc tttttctctt cactgagacg agccgacgag atgagttgat
     1141 aggcggcgac ggacgccaga cccatcatga gccagaccag gagtgtcttg tgtgactgcc
     1201 gcaataggaa cctcggcgtt tgggaatcgc tagcttactt aagtcgtcct ctttgagcta
     1261 gccaggggga aatacttaac cactacatcg acgacaacga gccagtggga agattattcc
     1321 accttgcggc gggaccgtcc aaacgactgg gcttcatctg cttcgatgcc gtcacggtgg
     1381 caaaggttca gcgacaaact cgccgaaaag ctgaagctgc ggcagcgccg ggtcgaagtc
     1441 ttcgcaaagg ccggcccgca cgccgagcag ccacagcggc tggtcgtttt tcgtcagcac
     1501 ccacagcttg gcgcgctcgt ctttgggcag ctttagatcc gtccacagat cgctgacttt
     1561 gcgatgtccc gccggcagac gcagcctgtc gcctggtcgc acccggcgca gaccgagcgg
     1621 aaaagccggt gccacaagac ggatcaagac ctgggacttg ccatcgaccg cagggtgttc
     1681 gttgggcgcg agcacgcgca gccggacgcg gcccagcccg atcgaatatt cgccggcaca
     1741 agcgatcgca cgttccggtg ctgaatcgat gatcggcgcg gtgtccggct ggtgccgccg
     1801 caagtacagc gtttcgtagc gtcgctcggc aaagaggcca tgcggcagat ccacgcgctg
     1861 gctgccatgt tgcgagctgg caagctgcaa aatcgcctgc cagtgcacgc tcgccagcgg
     1921 tcgcccgatc acttgctgta gcacccgcag cgcgagcggt cgtggcaacg cggcaagctt
     1981 ggttgtcgac aaggcggcaa gctggccagg gagcttgggt acctcgacgg ccaaaacgcc
     2041 aggaagctgc gtggcggcga gctgatcgag aagctcggca tcggcgcgca gctgatcggc
     2101 aagctgggcc aggtgggttt tctgcgcggg cgcgatggct gacaaggccg gcagcagttc
     2161 gtgacggacg gcgacccggc gaaagctgcg gttgtcgttg ctgggatcgt gcagcggcag
     2221 tggtgccagc acggacgccg cttgatcgat cagcgccagg acttgggttt tttcgaccgc
     2281 cagcagggga cgcagcagca gacagcccgg caagctctgg gacagtggcc gcgctttggg
     2341 gatgccacca agaccccgca gacccgcgcc cccggtgagc cgcagcagca cggtttccgc
     2401 ttggtcatcg agcgtatgtg ccgtcagcac cgcttgtgca tgggtcttgc gggccacgct
     2461 ttcgagcgct tcataccgtg cggtccgcgc cgcgctctgg atcgatgctg tgcgcggaac
     2521 cgcgacccgg acggactgcg cggaaagacc gagctgggtc gcaacggcaa ccacctgctg
     2581 cgcttccctg gccgcttctt tgcgcaggcc atgatcgacg ctcaccacct gcacatgcca
     2641 gtggtggcgc ggcgaaagca gccacagcgc aaccatcagc gccgtcgagt ccggcccacc
     2701 cgagcagccg agcacaagtc gcgcccccgg ccttatcccg gccccaagca gtgccttttc
     2761 acagtgggtg acgactgggc tttgccgaag cttgtgggca agctgcagaa gctgcgcggt
     2821 ctgcgtggac gcacagggga cggtattggg tggtggggtt cccattgcgg gttcggccag
     2881 gagccgggcc cgatgataca cttggtggtc atgaaagttc gtgcttgtcg cctgaaaacc
     2941 tgcgccgtag ggacgctgtt ggccggcctt gccgtcgctt ctcctttgtg ggcgggccgt
     3001 ggcgctgcag gtcctgtggc atcgtctgtg gcgacgcccg atctacccag tcgggaatcg
     3061 ctcgacgtgg tgaaaaagcg cgccagcgag cagcgcaaaa tgctggtgat cgaactgggc
     3121 gcggcgtggt gtacgccctg caaacagttt gaaagcgagg tcttgccaaa agagtcggtg
     3181 cgggtggcgc tcgggcaggt gtatttcgtt cgctacgatg ccgaaaccga ggtcggcaag
     3241 caggccgcgc gggcgctttc ggtcgaaggc tatccgacgt ttttggccct gcggacggac
     3301 ggggaagtcg ccagcatcgt ccagggcttc cagtccgaga cagagtttgt gcactggatc
     3361 tcgcaggtgg cgcctgactt tgaaccgagc gcaatgctcg acgagcggct gaaaaagaac
     3421 ccggctgacg gggaagcgat gctgcttgtc gcgctgcggc agcgcaaagc cggcaatctt
     3481 caggcggcgc tcggctggct ggaaaaagcc cgcgtggcca agaccgcttc cgcggacagc
     3541 caggccaaag ccgactggga gctgcgtcgg ctgcgactgc gacttctgct gccccgtcag
     3601 cagcttgtcg aacacctgga tcagtttccg tctggaccgc acaaagacga agcgctgcgc
     3661 gcacttttgc gccttggtcc gctggatgca gttgcccagg gtgccgtcgc ccgctatctc
     3721 gatcgggccc ttccgaccct ggcgcccgac cggctaaacc aactggtcta tcggctgctg
     3781 cgcgcccagg cgtttcctga agcagagcgg gtgacgcggc atctgctgag catcgacccg
     3841 aggagcccgt attacctgga tacgctggca gaaattttcc acctgcgcgg tgacagcaaa
     3901 gaagcgctgc ggctgtccgg ccaggcgctt ggccaggtca aaggggcaag tggcgaagcg
     3961 ctgcggcagt cgcttttgca aaaccaagcc cgctttgccc gcgcccgcaa agaaccaccg
     4021 gcggagctgg cgacgcctga cgacgagcta cagccgtggg aaaaagacta gctagcaggc
     4081 cgccgcaagc gggatggccg cgtggaacgt cagccgatag acgctgtttt ccgtttccac
     4141 gtagaccgag tcactgtccg ggtcggtcag cacgcgccta acgggcgtgg tcaccatccg
     4201 atggccgttt ggatcgcgaa agatcaccat gccgcgaccc accgtcggat agctgaccaa
     4261 aagaccgtgg tatacgcggc ctgcacaggc gctcggcttt tcccttgcgt cgcgcccatg
     4321 ttcacggatc ttgcgtgccg tgacgttaac catccgaaca ggtgcccctg accgacaatc
     4381 ttacggttcg acttgcgccg ctccatagac attgttgtaa gtgcgcaaga tcgctgcggt
     4441 tttgtttgtg gtaaggggcg gtatgtccga catgcaaagt gcgcggccag accttgcgca
     4501 agtggttccg caggcgccgt ggattcgccg gattccactg cgcagcccga cgctgcctcc
     4561 ggcgacccat accaacgcat atgtgattgg cgaaagcgac ttggtgattg tcgatccagg
     4621 gacgccgtgg ccggacgcgc aagcgccgct ttgggatgcg ctgcgcggtc ttcaatcggc
     4681 gggtcttgtg ccacgcgcga tcgtgctgac ccaccatcac ttggatcacg caagtgccgc
     4741 tgccgagcta tcgcgtgagt tttccctgcc agtgtgggcg catccccaaa caacagagcg
     4801 actgagcggt cggatcgagg tcgagattgc gcgttcgctg cacgacggcg agcttttgcc
     4861 gtttgggcct tctgggctgc gggtcttgca caccccaggt catgcgccag gccatgtctg
     4921 cctgctggat gaagcggggg gcggtttgat tgcgggggac atggtggcat cgcagggcac
     4981 gatcattgtg tcaccgcggg acgatggcga catggcgcta tatcttgcgt ctttgcgacg
     5041 gctgctttcg cttgggccgc tgcgggtctg gccggcgcac ggggtggccg tcgaagaggg
     5101 ggacaagctg cttgggcgct acctggacca ccggctgctt cgcgaagaaa aagtggtgcg
     5161 ggcgctgtcg tcgccggctc acacgataga cgcgctgctg ccgctggcct acgacgacac
     5221 gccggaagcg ctgtatccgc tggcgcggga atccctgctg gcccacctgc tggcgctggt
     5281 cagcagcgga cgggtcgcac gcaggccaca gccgagcggc gaagatcact ttgttctttt
     5341 gcacccttag ccagcggcgg tgtaagggtg cgaatgcgca cgagggcggg gcactacagc
     5401 ggatgcgcac ggcccttttc aggcggggcc aagttgtggc acacactgag cccaaagttt
     5461 tccggccaag accgtcaatg aggtacctgt catgagtcag caagaccccg agcgggcgca
     5521 gcttccgctg atcagccagc gcaagtccga acacatcgcg ctgtgtgcat cgggtgacgt
     5581 cgagtttcgc caaaagggca cgctgctcga ccaagttcag ttcgttcacc aggcgattcc
     5641 cgaacggcac tacgacgaaa ttgacctgtc gacgccgctg cttggcaaag tgctcaaagc
     5701 gccgattgtg ctgagcgcga tgaccggggg caccgatgaa gcgcagcaga taaaccacga
     5761 cctggcccgc gcggcggaaa cgctggggct tggctttggt ctggggtcgc agcgggcgat
     5821 gctggtgcgg cccgaaacga cccggacgtt tcaggttcgc gagcatgcag agacggcgct
     5881 ggtgctgggc aacatcggac tggtccaggc ggccaggatg tcgaccgccg aagtccggtc
     5941 gctgtgcaag acggtgtctg tcgatgcgct gtgcgtccac ttggcaaccg ggatggagct
     6001 ggtgcagccc ggcggggacc gcgacttccg cggcggtttg gatacgctgc ggcggctgca
     6061 cggcgagctg ggaattccca tcgtcgtcaa ggaaaccggc tgcgggctgt ctcgacaagt
     6121 cggcttgatg gtgcgcgaga tcggcatcac ggcggtggat gtcagcggcg cgggcggtac
     6181 gtcatgggtc ggggtcgaaa cgcaccgggc cgaaggcgtc gcgcgggcgg ttggcgaagc
     6241 gctgtgggac tggggcgtgc cgacggcggc atcggtgtct ttgctgtcgg atctgaacct
     6301 gcaaatcatc gcgacgggcg gtctgcgcag cggactcgat gtggcggcgg cgattgcgct
     6361 gggggcgcgg gctggcgggc tggcggcccc ggttctgaag gcgtggaaag ccggcggtta
     6421 cgacggagcg gtggaatacc tgcgcggcgt cattgaaacg gtgaaagcgg tgacgtttct
     6481 gaccggctgc cggacgccgg cagagcttgc gtcggcgccg cgggtgattg gatcgacgct
     6541 acgggcttgg caggaagggg ctcgctaggc cgatgctgaa aaccagcgct tgtggaaaag
     6601 tcatcctgct tggtgagcat gcggtcgtct atggcgtccc ggcgctgtgc ggtgcgctgc
     6661 aaggcggcgt cgaggtggag gtcattcccg gccagggtcg tctgatcgtg cccgagtggg
     6721 aagtggcgac gccgatgctg gctacgctgc tcgacccaac cactcgacat ggccactcgt
     6781 ctttggccga tgccctggtc gcgatcgtcg agtcgtcgtt tgccgcggtg tctcgtccgc
     6841 tttcggctgc ggacctgccg gttgattttg ttgcgcgctt tgcgattccg acgggcgctg
     6901 ggctgggttc atccgccgcg ctctcggtgg ccttggttcg cgccgtcgat cgcgctgcgg
     6961 agctgggact ttccgaatcg gacatcgatg ctgcggcgtt tgctgccgaa agagtctttc
     7021 acggctcgcc atcgggactt gatcacacgg tggcgcagcg cggtggcttt ggtttgtacc
     7081 ggcgcggcca cggtctgtcc gtgctgacgg gcacaccggc gttgcggctg tgcatcgggc
     7141 acaccggacg ggcccgcgat accaaaggcc gggtggcgcg cgtcgctgag ctgacaaagc
     7201 agcagcccga caagacggcg gccatttttg cccgcattgc cacgctggtc gaagagtccg
     7261 cggcggcgct ggctcggcat gactttgcgc gactgggcga cgcgatgaac gaaaaccagc
     7321 agcttttgtc tctgctggat gtgtcgtgtc cggaaatcga gcaggtctgt gcgattgcgc
     7381 ggaatgcagg ggcgcttggt gccaagctga ccggcggggg cggcgggggc tgcgtggtgg
     7441 cgttggcacc ggggtgcgaa gacgcggtcc tggctgcttg gcaagccgcg ggctattcgg
     7501 catttctcgt cgaggtgggc ggtgcgggcc agacggccaa agcggctggg ccggcggcaa
     7561 agacagggag cgtttcagaa caagccatgc atgcggttgc gagtgccaat accaacatcg
     7621 ccctggtgaa atactgggga aagtgcgatc ccaagctgaa cctgccggcg gtgccgagcc
     7681 tgtcgctgac gttggccggg cttacgacgc acaccgaagt aacgctcgat ccgggtcgcc
     7741 aagcggatga acttgtctta aacgacaaga gcgtgagcgg cgagccgctg cgcaaagtca
     7801 gtcggcacct ggaccggctg acgcgccacc tggggcttgc ggggcgaccg tttgctttgg
     7861 tgcgcagcca caacaacttc ccgacggcag cggggcttgc ttcatcggct tcggcgtttg
     7921 cggcgctgac ggtggccggc tatggtgcac tgcttggtga aaagtcgctg tcgacgccgc
     7981 ttgcgcgcag tgtgctttcg agcctggcgc ggcaagggtc gggatcagcg gcgcgttcgt
     8041 tgttcggcgg gctcgcggtg ctgggggtcg ggacgccggg acaagtcgat tcggcgctgg
     8101 ccagccagct tttgacgccg gaagagtggc cggacctgcg gctggtgatt gg
//
LOCUS       DAORGN010000217         7998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4088_length_7998_cov_4.824625, whole genome shotgun sequence.
ACCESSION   DAORGN010000217 DAORGN010000000
VERSION     DAORGN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4088_length_7998_cov_4.824625"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PKL17_18285"
     CDS             complement(<1..1718)
                     /locus_tag="PKL17_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46738.1"
                     /translation="MRRSYLLVAVALFAAWFLNLPGFVGGGAIGIPSAAARVAVGGAR
                     GPQGGELSGGGDPAAAALLAELWAGRTPPAVPEHLRPWIPWVLHDNEQALCPRLSADD
                     DGSEPVCAWPSRLRLELRDKGGGFVQVVRTYRRMAVSLPGSGKHWPQDVRVDGQPAIV
                     LSQDGSADEDAQPVIYVGPGSHMILGNFQWDELPESLEAPKDVGILTLLLRGSQILHP
                     NRDDDGRVFLQKEDAGEEEEDSLDVTVHRKLTDGVPLELTTRIELRVSGKRREVSFER
                     ALPDKFVPMSISGPLPARLESTGTLRIQVRPGTHVVSVTARHQGPAIEIQRPQPPARW
                     PADEAWVFEAQPSLRLLSIEGVEALDPQQTTLPAEWKKLPCYAMKQGATMRLSVQRLG
                     DSQPLQDELRLDRQIFLDFDGRGYTIADRLTGKLRRAWRLEVGEGTELGRVSVEGKDQ
                     LVTIGKESGRAGVELRQGALSISADSRMPFGSEFSAVSWNAEFHEVHSTLHLPPGWQL
                     FYASGADDIPDTWLRRYHLMGLFLSLLAGMAVWKLYGPRWSLLTLGGLLLACPVSEQP
                     HVAVLAV"
     gene            1941..3479
                     /locus_tag="PKL17_18290"
     CDS             1941..3479
                     /locus_tag="PKL17_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46739.1"
                     /translation="MSAPASTAKTADTMIGRKIGHYAITRRIGQGGMGVVYEAVHDKI
                     KQRVAIKVLHKEMSQDEKVVQRFFNEANAISMAQHSSIVKIMDYGQLDDGTAYIMMEF
                     LDGEPLLSRIERAQEHGTGLGLHMVVELGRQTATALGLIHEKNIVHRDLKPENIFIVP
                     DTVAPLGERVKLLDFGIAKFLDGPVRKTTVGMILGTPLYMSPEQCEGREDLSPKVDVY
                     ALGAMLFEMVTGRLPFQADTAAALMRQHMFKAAPRLRDYAQTAPAELDDLIAEMLRKE
                     PADRPVMTDIARRLEALIPHVTGKNTPLVEGVQMPKVARKTGISEADPLAATVGDSSR
                     HSGPSSRPLGSSGSQSGLSQSQTGSIGSEPKSKLSVPILLGAIGVLLVGLLLAIFALP
                     PKNPAPPQSAAKIQTPPVPPEPPQKNPQAATSPSETSAKTTVSSADPKNKAAAEPVAD
                     TAPADSAAKQIKKKPVKVGRPVKPPGTESAAAAPPPPVVEKTAKPTGKTEKTGGDIGV
                     WRDP"
     gene            3502..4143
                     /locus_tag="PKL17_18295"
     CDS             3502..4143
                     /locus_tag="PKL17_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46740.1"
                     /translation="MKTQRLHLACLFLVAAAIFLSARVHAAPPSSGGSLTAEQHIEEA
                     QKLFKEQQFSEAGEHLLAAHETKPQPVLLFNAGQAYRKALRPIEARNAYQKLIDDYPS
                     HPLVPESKGYVQTLDALIAEVKEKQQIELALTEQKSKTEKELAEERARREEVQKELSK
                     YKKPFYKKAWFWLLVSGVVVGGGVVALAVWDQYRQSQTKGGTQHLPFSLTVNY"
     gene            4161..5081
                     /locus_tag="PKL17_18300"
     CDS             4161..5081
                     /locus_tag="PKL17_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46741.1"
                     /translation="MGTRTIRQAMLLLPLLSTSCWDRPQFVLVLDGLDASYKKLYVSA
                     TLTTPGSDGSSGGGPGNASTQTVFDLNGTSGSFDFGLTLDVPSNANEQGRVGVAELMF
                     ADDKCIKRIVTVPLSGTKNGALTKYTVNLKPAAGEAPQFEEVPIMPVGSNGSAKSCYP
                     IQRPVITAVTRELNGTWGSAVSRLKIHGWGFVKGTTVSARVASGRPDTVMCGTLGVQC
                     SAWDDTDMSKSISEATTTHITVDLKDFTPKLAPAILTLGKLLPPQVSKLGFLTDARPA
                     NMATALFPFSVAVTATADGQTKSDTYAEFP"
     gene            complement(5100..5528)
                     /locus_tag="PKL17_18305"
     CDS             complement(5100..5528)
                     /locus_tag="PKL17_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46742.1"
                     /translation="MKRGAMDLLYVGLGLGLVLLIAGATSCSVSSIEQPDLALACTAT
                     GPTACKADLAPPPPPDGMPPPDGMMAAADGMLGPASCTSDTDCKTACPMGSKGCKCAQ
                     LLTGTSKGCSPTCATTADCPMPPMGTVSCDTTQGACLVKP"
     gene            5651..6382
                     /locus_tag="PKL17_18310"
     CDS             5651..6382
                     /locus_tag="PKL17_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK46743.1"
                     /translation="MSKGAPNLPGAADPKGSLSADSLPVAIPADVSRRSFLKQTGGAA
                     SAAALLSLPTPGAAAPGGTAAPAGPALVLGPGAVPVTLLVNGKAQKLMIEPSETLATV
                     LRDRLGLTGTKVGCDRGGCGACTVHVAGSPQLACMTLALDVAGMAGKDALAITTVEGL
                     AKGAQLHPLQQAFIEKDALQCGYCTSGMLMSCAALHEKAKAEGKLAELGEADVRAAIA
                     GNLCRCGAYPHIISAALAGAKGGAK"
     gene            6379..>7998
                     /locus_tag="PKL17_18315"
     CDS             6379..>7998
                     /locus_tag="PKL17_18315"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK46744.1"
                     /translation="MSRIDLPVGIVGAGLSSEPREVPDGTPPAWPENQKLAQVGKSPP
                     RIDGTEKVTGRARYTSDVSRPGLLIGRVLRSPHPSARVHRVDVSEALKQPGVKAAVVL
                     PSLLGVADDGSAKGTQAARVLYYGQPLAAVAAQTKAQAEAALAKVKVDYDVLPFVIDP
                     SEARKPSAPKVFEQPVTSRGSAGGGGSKDKLPQTGNVRGPKRSTARGRDTDKQAAQVT
                     QALLAGPQGVRATYRTQVQTHSALETHGLLAEWNPAGDELTVWASTQGIFSVRDELAE
                     YLGLSPTKVRVITDYTGGGFGAKFGAGNYGVLAALLAKDAKSPVRIMLDRREEHLAVG
                     NRPGSEQTVALSASADGKLTAIEVKGFGFGGAGAGAGFGGPALSLYDAPAIYVEESDV
                     FTHSGPLAAFRAPGHPQGCFALEQAMDELAERLHLDPLVLRDKNDTHAARREERKRLA
                     AAFGWGSRTPAGQGPRLGKLGEIRRGIGVAQGCWYHLVNLESSAEVRIHRDGSVEVLT
                     GVQDIGGGIRTPLAQVVAEELQVPLSKVKVTI"
BASE COUNT         1654 a         2689 c         2389 g         1266 t
ORIGIN      
        1 acggccagca ccgccacatg gggctgctct gacaccgggc aagccagcag aagccctccc
       61 agcgtcagca gtgaccagcg cggtccatac agcttccaca ccgccatccc agccagaagc
      121 gacaggaaca gccccatcaa gtgatagcgc cgcagccagg tgtcggggat gtcgtccgca
      181 ccgcttgcgt aaaagagctg ccaacccggc ggcaagtgca acgtgctgtg gacctcgtgg
      241 aattcggcat tccatgaaac cgcggaaaac tcggaaccaa acggcatccg gctgtcggca
      301 cttatcgaca gggcaccttg ccgcagctcg acgccggccc ggccactctc tttgccaatc
      361 gtcaccagct ggtctttgcc ttcgacggac acgcggccaa gctcggtccc ctcgccgact
      421 tccagacgcc aggcgcggcg cagcttgccg gtcaaacggt ccgcgatggt gtagccgcgg
      481 ccatcgaagt cgaggaaaat ctgccggtcc agccgcagct cgtcttgcag gggttggctg
      541 tcgccgagcc gctgcaccga cagacgcatc gtcgcccctt gcttcatcgc atagcacggc
      601 agctttttcc attcagccgg caaggtcgtc tgctgcgggt caagcgcttc gaccccttca
      661 atgctcagca gccgcaacga cggctgggct tcaaacaccc acgcttcatc ggcaggccag
      721 cgggccggcg gctgtggtcg ctggatctcg atggccgggc cctgatggcg cgccgtcacc
      781 gaaaccacat gcgtgccggg ccgcacctgg atgcgcagcg tccccgtcga ttcgagccgc
      841 gccggcagcg ggccacttat cgacatcggc acaaacttgt cgggaagtgc tcgctcgaat
      901 gacacctcgc gacgcttgcc ggacacacgc agctcgatgc gggtcgtaag ctcaagcggc
      961 acgccatcgg tcagcttgcg atggacggtg acatccagcg agtcttcctc ttcttcgccg
     1021 gcgtcttctt tttgcagaaa cacccgcccg tcgtcgtcgc ggttcggatg caggatctgc
     1081 gaaccacgca gaagcagcgt tagaatgccc acgtctttgg gcgcttccag cgattcgggc
     1141 agctcgtccc actgaaagtt ccccaggatc atgtgcgatc cgggccccac gtagataacc
     1201 ggctgggcgt cttcatcagc gctgccgtct tgcgacagca caatcgccgg ctgaccatcg
     1261 acgcgcacat cttgcggcca gtgcttgccg ctaccgggca gcgataccgc catccgccga
     1321 taggtgcgca caacctggac aaatccgccc cctttgtcgc gcagttccag gcgcagacgc
     1381 gacggccacg cacagaccgg ttcgctgccg tcatcgtccg ccgaaagccg cgggcacagt
     1441 gcctgttcat tgtcgtggag aacccaagga atccacggtc gcaggtgttc gggcaccgct
     1501 ggcggggtac gccccgccca cagctcggcc agcagtgcgg ctgccgccgg atcaccgccc
     1561 cccgaaagct cgcccccttg gggtccccga gcccccccaa cagccactcg agctgcggca
     1621 ctggggatgc cgattgcgcc cccgccgaca aagcccggca gattcagaaa ccatgcggcg
     1681 aaaagcgcca cagccaccag caaatacgat ctgcgcatgt tccccccaac gcgcgtcatt
     1741 gcaaatcagt ccatcatatc gcggaactgc cgaaacaggt ggggtggtca tacagcctga
     1801 tgtgagccgg gcattcgggc ttggcccagc ggccagccgg cgtgtattct caaaggttga
     1861 gcttgcggcg caataggctc ttaggaaccc agcttacgtc tgttgcgtcc atccacagaa
     1921 gcatcgaagg acaggcaccc atgtccgctc cagcgagcac ggcgaagacg gcagacacga
     1981 tgataggccg caagatcggc cactacgcga tcacgcggcg gatcggccaa ggcggcatgg
     2041 gagtcgtcta cgaggcagtc cacgacaaga tcaagcagcg cgtcgccatc aaggtgctgc
     2101 acaaggaaat gtcgcaggac gaaaaggtgg tgcagcgctt cttcaacgag gccaacgcca
     2161 tcagcatggc gcagcactcg agcatcgtga agatcatgga ctatgggcag ctcgacgatg
     2221 ggactgccta catcatgatg gagttcctcg atggcgagcc actgctgtct cgcatcgagc
     2281 gcgcccaaga acacggcacc gggcttgggc tgcacatggt tgtcgaacta ggacgacaaa
     2341 cagcgacggc gctcggtctg atccacgaaa agaacattgt ccaccgtgac ctcaaaccgg
     2401 aaaacatttt cattgtgccg gacacggtgg cgccgctcgg ggaacgggtg aagctgctcg
     2461 atttcgggat tgccaagttc ctggatggac ccgtgcgcaa aacgacggtg ggaatgatcc
     2521 tcgggacgcc gctctatatg tcacccgagc agtgcgaagg gcgcgaggat ctaagcccca
     2581 aagtcgacgt ctacgcactt ggcgccatgc tgttcgagat ggtcaccggg cggctgccgt
     2641 tccaggccga caccgccgcc gccctgatgc gacagcacat gttcaaggca gcaccgcggc
     2701 tgcgcgacta tgcacagacg gcaccagctg aactagatga tctgatcgcc gaaatgctcc
     2761 gcaaagagcc cgccgatcgc ccagtgatga ccgatattgc gcgacggctt gaagcgctga
     2821 ttcctcacgt aaccggcaaa aacacgccgc tcgtcgaagg cgtccagatg cccaaggtgg
     2881 cacgcaagac cggcataagc gaagccgatc cactggccgc gaccgtggga gacagcagcc
     2941 gccacagtgg gccttcgtcc agacctctcg gcagcagcgg atcacagtcc ggcctttcac
     3001 agtcccagac gggctcgata ggcagcgagc caaagagcaa gctaagcgtg cccatcctgc
     3061 tcggggcgat aggcgtcctg ctcgtgggtt tgctgctggc gatctttgcg ctgccgccga
     3121 aaaacccggc ccccccccag tccgccgcga agattcaaac gcccccggtc cccccggaac
     3181 cgccgcaaaa aaacccgcaa gctgccacgt ccccgtcaga aaccagcgca aagacgacgg
     3241 tgagcagcgc ggacccaaag aacaaagccg cagccgaacc agtcgccgac actgcgcccg
     3301 cagacagtgc cgcaaagcag atcaagaaaa agccggtcaa ggtcggcaga cccgtcaagc
     3361 caccgggcac agagtccgca gcagcggccc ccccgcctcc tgtcgtcgaa aaaacggcca
     3421 aaccgaccgg caaaacggag aagactggcg gggacatcgg tgtgtggcga gatccgtagt
     3481 tgctctgcgc aggatattcc gatgaagacg caaagactcc acttggcttg cctgtttcta
     3541 gtcgctgccg cgatcttcct ttcggcccgc gtccacgccg caccgccatc atccggcggc
     3601 agcctcaccg cggaacagca catcgaggaa gcacaaaagc tgttcaaaga gcagcagttt
     3661 tccgaagcgg gagagcatct gctggccgca cacgaaacca agccgcagcc cgtgctgctg
     3721 tttaacgccg gacaggccta ccgcaaagcg ctccggccca ttgaagcgcg gaatgcctac
     3781 cagaagctga tcgacgacta tccgtcccat cccctggtgc cagagtccaa aggctacgtg
     3841 cagacgctgg acgcgctgat tgccgaggtc aaagagaagc agcagatcga gcttgcgctc
     3901 accgagcaga agagcaaaac cgaaaaggaa ctggctgaag agcgcgcccg acgcgaagaa
     3961 gtccaaaagg agctgagcaa gtacaagaag ccgttctaca aaaaagcctg gttttggctg
     4021 ctcgtctcgg gtgtggtggt cggcggtggc gtcgtcgccc tggcggtctg ggatcagtac
     4081 cggcagtcgc agaccaaggg agggacccag cacctcccct tctccctcac tgtgaactac
     4141 tagaacaagg agccaccgag atgggaacgc gcacaatccg ccaggccatg ctccttttgc
     4201 cgctgctttc gacgagctgc tgggacagac cacagtttgt gctggtgcta gacgggctcg
     4261 atgccagcta caaaaagctc tacgtaagcg cgacgctcac cactccaggc agcgacggaa
     4321 gtagcggggg aggcccaggc aacgccagca cgcaaacggt atttgaccta aacggcacga
     4381 gcggcagctt cgacttcggt ctcacgcttg atgtcccaag caacgcaaat gaacaaggcc
     4441 gcgtcggtgt ggccgagctg atgtttgccg atgacaaatg catcaagcgg atcgtcaccg
     4501 tcccgttaag tggcacaaaa aacggagccc tgaccaaata caccgtgaac ctcaagccag
     4561 cagccggcga ggccccccag tttgaagagg ttccgatcat gcccgttggc agcaacggca
     4621 gcgccaagag ctgctatccc atccagcggc ccgtcatcac cgcagtcacc cgcgagctga
     4681 acggcacctg gggttcagcg gtcagtcggc tcaagatcca tggctggggt tttgtaaaag
     4741 gaaccaccgt cagcgcccgc gtcgccagcg gcagacccga caccgtcatg tgcggcacac
     4801 tcggggtcca gtgcagcgct tgggatgaca ccgacatgtc gaagagcatt tccgaagcga
     4861 caacgacgca tatcaccgtc gatctgaagg acttcacgcc caagctggca ccagccattc
     4921 tgacccttgg caagctcctg ccgccccaag tgtccaagct tggctttctg accgatgcac
     4981 gcccagccaa tatggcgacc gccctgttcc cattttcggt cgcggtgact gcgaccgccg
     5041 acggtcagac caagagcgac acctacgccg agttcccgta gccacggccc catcacgcct
     5101 cacggcttga ccaggcaagc gccctgcgtg gtgtcgcagc tcaccgtgcc catcggcggc
     5161 atcggacagt cagccgtcgt cgcgcaggtc ggggaacagc ccttgctcgt gcccgtcaaa
     5221 agctgggcac acttgcagcc tttggaaccc atcggacagg ctgtcttgca gtcggtgtcg
     5281 cttgtgcagc tcgccggtcc cagcatgcca tctgcagccg ccatcatccc atcaggcggc
     5341 ggcatcccat ccggtggtgg cggcggtgcc agatccgctt tgcaggctgt cggcccagtt
     5401 gccgtgcacg ccagcgcaag gtccggctgc tcgatggacg aaaccgaaca gctcgtcgcc
     5461 cccgcgatca gcaacaccag cccaagtccc agtccaacat acagaagatc catcgcgcct
     5521 cgcttcatgt gtcgtgcctc ccaatcactc agacccgcgg cccgctacgc ttgggcaagc
     5581 ggcaaaaccc cggcgcaaac aatcgtcttg gcgtttcctt ccggactcgc ttttttgtat
     5641 tgtcgcctct atgagcaagg gcgctcccaa tctccccggt gcagcagatc ccaaagggag
     5701 tctgtctgcc gattccctgc ccgtcgcgat tccagccgat gtatcgcgcc ggagctttct
     5761 taagcagacc ggaggcgccg cttctgccgc cgcgcttctt tcgctgccca cgccaggtgc
     5821 tgccgcccca gggggaaccg ctgctcccgc tggaccggcc cttgtgctgg gcccaggtgc
     5881 agtcccggtc acgcttttgg tcaacggcaa ggcccaaaag ctgatgattg agccgagcga
     5941 gacgctggcc accgtgctac gcgaccggct cggactcacc ggaaccaagg tgggctgcga
     6001 tcgcggcggc tgcggcgcct gcacggtcca tgtcgccggc agcccgcagc tcgcctgcat
     6061 gaccctggcg ctcgacgtgg caggcatggc cggcaaggac gcgctggcga ttaccacggt
     6121 cgaggggctt gccaaggggg cccagctcca cccgctccag caggccttca tcgaaaaaga
     6181 cgcgctccag tgcggctact gcacgtccgg catgctgatg agctgcgcgg cgctccacga
     6241 aaaagccaaa gccgaaggca agctcgccga gcttggcgaa gccgatgtcc gcgctgcgat
     6301 tgccggcaac ctctgtcgct gcggcgccta tccccacatc atcagtgccg ccctcgctgg
     6361 tgccaaggga ggtgccaagt gagtcggatc gatcttcccg ttggcatcgt cggcgcaggg
     6421 ctcagcagcg aaccgcgtga ggttccggac gggacaccac cagcgtggcc ggaaaaccaa
     6481 aagcttgcgc aggtcggcaa gtcaccgcca cgcatcgacg gaaccgaaaa agtcaccgga
     6541 cgagcgcgct acacaagtga cgtcagccgg cccggactgc tgatcgggcg ggtgctccgc
     6601 tcaccgcatc caagtgcacg ggttcaccgc gtcgatgtca gcgaagcgct caagcagccg
     6661 ggcgtcaaag ccgccgtcgt gctgcccagc ctgcttggtg tcgccgatga tggcagcgcc
     6721 aaaggcaccc aagcggcgcg cgtgctgtac tacggacaac cgctcgcagc ggtcgccgcg
     6781 cagaccaaag cccaggccga agcagcgctc gcaaaagtca aagtcgacta cgacgtgctg
     6841 ccgtttgtga ttgacccaag cgaagcgcgc aagccgtccg cgcccaaagt cttcgagcag
     6901 ccggtgacat cgcgtgggtc agcaggtggc ggtggcagca aagacaagct gccccagaca
     6961 ggcaacgtcc gcggtcccaa gcgcagcacg gcgcgcggtc gcgacaccga caagcaagcc
     7021 gcccaagtca cccaagccct gctcgcgggg ccacagggag tccgggcgac gtatcgcacc
     7081 caggtccaga cccactcggc gctcgaaacc cacggccttt tggcagagtg gaaccccgct
     7141 ggtgatgagc taacggtctg ggcatcgacc cagggaatct tctcggtacg cgacgagctg
     7201 gctgaatacc tgggcttgtc accgaccaaa gttcgggtca tcaccgacta cacgggcggt
     7261 ggctttggcg ccaagttcgg cgcgggcaac tacggtgtgc tcgctgccct gctggccaag
     7321 gacgccaagt cgccggttcg gatcatgctc gaccgtcggg aagaacacct cgccgtcggc
     7381 aaccgcccag gctccgaaca gaccgttgcc ctgtctgcca gcgccgatgg caagctcacc
     7441 gcgatcgaag tcaaagggtt tggctttggc ggcgctggtg caggtgccgg gtttggcggc
     7501 ccggcgctgt cgctttacga cgccccggcg atctacgtcg aggagtccga tgtcttcacc
     7561 cacagcggac cgctcgcagc attccgcgcg ccaggacatc cgcaaggctg tttcgctctg
     7621 gaacaagcga tggacgagct ggccgagcga ctgcacctag accccttggt cttgcgcgac
     7681 aaaaacgaca cccatgcagc gcgacgcgaa gagcgcaagc gactggcggc ggcattcggc
     7741 tggggaagtc gcacacccgc tggacaaggg ccacggctcg gcaagctggg cgaaatccgt
     7801 cgcggcatcg gcgtcgcaca gggctgctgg tatcacctgg tgaacctcga atcgagtgcc
     7861 gaagtgcgca ttcaccggga tggcagcgtc gaagtcctga ccggcgtgca agacatcggc
     7921 ggcggcatcc gaacccccct ggcccaagtc gtcgccgaag agctgcaagt cccgctcagc
     7981 aaagtcaaag tcaccatc
//
LOCUS       DAORGN010000218         7958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4128_length_7958_cov_6.385170, whole genome shotgun sequence.
ACCESSION   DAORGN010000218 DAORGN010000000
VERSION     DAORGN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4128_length_7958_cov_6.385170"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1756
                     /locus_tag="PKL17_18320"
     CDS             20..1756
                     /locus_tag="PKL17_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46745.1"
                     /translation="MRVSFAAAVCTWGLVTCAGLTAQAAEPTDVASSFEFENKKPFGF
                     RAGATYNYTYKTASIGRESLPLLQSPTTRNYLLTKYGGTELARTERVPDLLYTQKRQT
                     LMAELAIGVFRDLEIGVRLPIVLRDERSYNLDPNAGWNNCPAPVSDSSGGGSGWDCVA
                     YASSTYLDGIYPIEPADLRGSTIFAPPVRGATGLKSTGLDGFDTINLWLQGAPVSQKR
                     DPSKPTWVIGVEYQLSIGNVMGYDNTRAYLGYDETASPSAAAQGLVSNANTSEKGWRG
                     VSDGLDRLVARTALSHKFKYVDPYFSLAYILPIARRSDTPWSVDYGFQQKRPSPQQQA
                     NMVFGFEATPWEQKAKGHRLALDFRGGLNFTFLGRGYSEAWELLASSNALICDDQTAL
                     PPAFNYKDANAYDALPPEPGKASGGTVTQGTFNPACRTPGATPTNTPHQGYRLPNASP
                     YYQKPYTGITLIENYMTFTADAGVILELFRHMRLRLGFTYARTQGHLITTDDAGTTSY
                     PSNVDRNNANNRVITIPPASNPVCNSNRVDLSCPMDWNPAYRAVINQPGRRYYVDDIN
                     TLGGSAMLQGYW"
     gene            complement(1747..2364)
                     /locus_tag="PKL17_18325"
     CDS             complement(1747..2364)
                     /locus_tag="PKL17_18325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNK46746.1"
                     /translation="MLALRERKLVALAFGVWAFGAWPVAGSAKDAGTVIARSLPATSG
                     VHTVAETVLEAPAAKVAAMLAEPNNFIPLFPAHSVEVLSAHADRRVVSVEMRKPWPIG
                     SVKWVEEVVSLEDPQDHAFIIERTAQPGYFRRLVSRWRVEPLTDAEHTRCKVTYDVTL
                     ELARWAPEWILRRNHVGGIKETMERLRSLVLKEKLPDTVAEANHQ"
     gene            2470..2916
                     /locus_tag="PKL17_18330"
     CDS             2470..2916
                     /locus_tag="PKL17_18330"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNK46747.1"
                     /translation="MSVRFSRWMMMGALFVGGFSALPFAQAEGSIAGAVSGKPFSVTV
                     AQVTAAKGQPVKAEVVVKPGAGYHINEEFPTKLSLKAPAGVTLAKADLGKADAKMSKD
                     ECRFEVTLTGNEAGQKTVPGELKFAVCTDTTCDPQKTAVSIALSVK"
     gene            2923..4278
                     /locus_tag="PKL17_18335"
     CDS             2923..4278
                     /locus_tag="PKL17_18335"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNK46748.1"
                     /translation="MPIPLPAHNSAFGDRHQGGARPEFRFYFDIVCPYAYLASTQVEG
                     FAERLGARIDHRPILLGGVLKSLASTPAAGPPAKQAMTRVDVARWAQHFGVELHFPAE
                     HPRRTVEAMRLLTWAPRLSIPSLMAALYRAYWVEGRDIADVEVLLDIAESVGLLRSEA
                     QKGLLSPAVSQELRRQTDAAIADGVFGVPTFIVEGESGPRLFFGQDRLIFVEEALRSH
                     PLMRDEGSTRPPRAVAPTPIGEYGAQVANQARKLTFVYDLSSPFAYLASTQVEKLAAA
                     CGAEVEFFPILLGGLFRSIGTPMVPISTYPESKRRHALEDMSRWAHHYGVSFHFPSRF
                     PINTVAALRLLLLAGDNIAPLTHTLFRLYWVGDQDLGDVQVLERALSEVGLDPRLIDR
                     VGEPEVKAKLLANTKQAEEWGCFGVPSFLVAQHHGKAELFFGQDRLLFVEKALRRGTS
                     H"
     gene            complement(4351..4548)
                     /locus_tag="PKL17_18340"
     CDS             complement(4351..4548)
                     /locus_tag="PKL17_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46749.1"
                     /translation="MVYTGVKVFSATKAREREELGENISRWLRSNPSLRVVDKVVTQS
                     SDREFHCLTITLFYVKEPKAT"
     gene            4822..6015
                     /locus_tag="PKL17_18345"
     CDS             4822..6015
                     /locus_tag="PKL17_18345"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNK46750.1"
                     /translation="MYVTSKDLWFVAAKRTPFGTYGGTLKDFTATDLAVHAAKAAIAA
                     GGVATADIDQVIFGNVQQSSADALYLARHIGLRSGVPVPAPALTLNRLCGSGFQAVIS
                     AAEQILLGSATTVLCGGTESMSQAPHVLRGARWGWPLGKPPAISDTLWDALTDTFTGM
                     PMAMTAEKLAEQYKISRTECDEFALTSQKRWGEAQASGCFEAELAPIEITSKKGTVRF
                     AQDEHPRPQTTIEGLAKLPTVFKKDGVITAGNASGICDGAAALILTTREHAESKGYKP
                     LGRLIGWGISGCDPTVMGIGPVPAIRNLLASTRTQVSDYDLFDVNEAFAPQFLAVAKE
                     LGLPLAQSNVCGGAIALGHPLGASGARITANLLHELRRRGGKRAIGGACIGGGQGIAI
                     AIESV"
     gene            6039..7298
                     /locus_tag="PKL17_18350"
     CDS             6039..7298
                     /locus_tag="PKL17_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HNK46751.1"
                     /translation="MTQQAASDIIYDWNLHDTHAPLWPAKFSLFDETLRDGLQSPSVH
                     DPSIEDKIQLVLLLDRIGVEYVDIALPGAGPRAVEDGLRLACEIRDRKLRIRPACAAR
                     THLNDVRPIVEISQKAGIAIEVMTFIGSSPIRQFAENWDKERILKMSAEAVSFAAQNN
                     LPCTYVTEDTIRSRPEMLAPLFKNAIDSGAHRLCLCDTVGHATPTGVDSLVRFARSVI
                     VGMGLDGKIGIDWHGHNDRGLAVINSLSALRAGADRIHGTVLGVGERVGNAALDQLLV
                     NLYLMGALPDDRDLSSLDALCTLISRACQVPIPYNYPVFGTDAFRTATGVHAAAIIKA
                     ERKGDAFLADRIYSGVPAAMFGRRQEIEIGPMSGESNVAYWLQRNGIAASQDAVKRVF
                     AKAKSANRLLSTAEILAVVQATDCQST"
     gene            7409..7612
                     /locus_tag="PKL17_18355"
     CDS             7409..7612
                     /locus_tag="PKL17_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46752.1"
                     /translation="MSDKNKFNGVKVFSATMAQERDQLGEKVTAWLAERPELRVVDTI
                     VTQSSDEAFHCLAITLFYQDDRV"
BASE COUNT         1577 a         2473 c         2435 g         1473 t
ORIGIN      
        1 caagcggtac cgtttcgcta tgcgagtctc tttcgccgcc gctgtctgca cctgggggtt
       61 ggtcacctgt gcaggcctga ccgctcaagc tgccgaacca accgacgttg ccagctcgtt
      121 cgagttcgaa aacaagaagc cgttcggttt ccgcgctggc gccacgtaca actacacgta
      181 caagacagcc agcattggcc gtgagtcgct gccgctcctc cagagcccga cgactcggaa
      241 ctacttgctg accaagtacg gcggtacgga actcgcgcgg accgaaagag tccccgacct
      301 tttgtacacc cagaagcgcc agacgctgat ggccgagctt gccatcggtg tgttccgcga
      361 cctggaaatc ggcgtcaggc tgccgatcgt gctgcgcgat gagcgctcgt acaacctgga
      421 cccaaacgcc ggttggaaca actgtccagc cccggtgagc gacagctcgg gcggcggttc
      481 gggttgggac tgcgtcgctt atgcgtcgtc gacgtatctg gatggcatct acccgattga
      541 accggcagac ctgcgcggat cgacgatttt tgcgccgcca gtccgcggag caacgggtct
      601 caagtcgacc gggctcgacg gctttgacac gatcaacctg tggctgcaag gcgccccggt
      661 ttcgcaaaag cgcgacccgt cgaagcccac ttgggtcatc ggcgtcgagt accaactgtc
      721 gatcggcaac gtgatgggct acgacaacac gcgcgcctat ctcggatacg acgaaacggc
      781 aagcccgtcg gcagcagcgc aaggcctggt atcgaacgcg aacaccagcg aaaagggctg
      841 gcgcggcgtc agcgacggtc tggatcgctt ggtcgctcgc acggcacttt cgcacaagtt
      901 taagtatgtc gatccgtact tctcgctggc gtacatcctg ccgatcgcgc gtcgttccga
      961 cacgccatgg tcggttgact acggtttcca gcagaagcgt ccgtcgccgc agcagcaggc
     1021 caacatggtc ttcggcttcg aagcgacgcc gtgggaacag aaggccaaag gtcaccggct
     1081 cgcgctcgat ttccgaggcg gactcaactt caccttcctg ggtcgtggtt attcggaagc
     1141 gtgggaactt ttggcatcgt caaacgcgct gatctgcgac gaccagaccg cgctgccgcc
     1201 cgcgtttaat tacaaggacg ccaacgccta tgatgcgctg ccgcccgagc ccggcaaggc
     1261 aagtggcggt acggtgacgc agggcacgtt caatccggcc tgccgcacgc caggtgcgac
     1321 gccgacgaac acgccgcacc aaggctatcg actgccgaac gccagcccgt actaccagaa
     1381 gccgtacact ggcatcacgc tgatcgaaaa ctacatgacg tttaccgccg acgcgggcgt
     1441 catcctcgag ctgttccgcc acatgcggct gcggcttggc tttacctatg cgcggaccca
     1501 gggacacctg atcaccaccg acgacgccgg tacgacgagc tatccgtcga acgtcgatcg
     1561 caacaacgcg aacaaccgcg tcatcacgat accgccggcg tcgaacccgg tctgcaactc
     1621 gaaccgcgtc gacctgtcgt gcccgatgga ctggaacccg gcctaccggg cggtcatcaa
     1681 ccagccgggc cgtcgctact atgtcgacga catcaacacg ctcggcggct cggcgatgct
     1741 ccagggctac tggtgattcg cttccgccac cgtgtccggc agcttttcct ttagcaccag
     1801 gctccgcagt ctctccattg tctctttgat tcccccgacg tggttgcgcc gcaaaatcca
     1861 ctcaggcgcc cagcgcgcaa gctctaacgt gacatcgtag gtgactttgc atcgggtgtg
     1921 ctccgcgtcg gtgagcggct cgacccgcca gcgcgacacc aggcggcgga agtagccggg
     1981 ctgggcggtg cgctcgatga tgaaggcatg atcttgcgga tcttcgagcg aaacgacctc
     2041 ttcgacccac ttgacgctgc ctatcggcca aggcttgcgc atttcgacgg aaaccacccg
     2101 ccgatcggca tgcgccgaaa gaacctcgac gctgtgggcc ggaaacagcg ggatgaagtt
     2161 gttcggctcg gcgagcatcg ccgccacctt ggctgctggc gcctcgagca ccgtttctgc
     2221 gacggtgtga acgccagacg tagccggcag cgatcgcgca atcaccgtcc cggcatcttt
     2281 tgccgatccg gcgacgggcc acgccccaaa cgcccaaacc ccaaacgcca acgcaacaag
     2341 cttcctttcg cgaagtgcca gcatgtgggg aacggtacga acctgttatg tcgcggtcaa
     2401 ccgcaaatca aatgaaggac tggcatggcg gtacgtcgtc cctatactga gggccattcg
     2461 gaggatgtca tgagcgttcg attctctcgc tggatgatga tgggtgcgct tttcgtcggt
     2521 ggtttttccg cgctgccgtt tgcacaggcc gaaggcagca ttgccggcgc ggtttcaggc
     2581 aagccgtttt ctgtgacggt cgcgcaagtg acggcagcca aggggcagcc ggtgaaggcc
     2641 gaggtggtgg taaagcccgg cgctggctat cacataaatg aggaattccc gaccaagctg
     2701 tcgctcaagg cacccgccgg ggtcacgctg gccaaggccg atctaggcaa agccgacgcg
     2761 aagatgagca aagacgagtg ccgctttgaa gtcacactga cgggcaacga agcgggccaa
     2821 aagaccgtgc cgggcgagct aaagtttgcc gtctgcaccg acaccacctg cgatccgcaa
     2881 aaaaccgccg tcagcatcgc tctcagcgtc aagtaagccg ccatgccgat cccgctgcct
     2941 gctcacaact cggcgtttgg cgatcgccac caaggcggcg cccgtcccga gtttcgtttc
     3001 tacttcgaca tcgtctgtcc atatgcctac ctggcatcga cacaggtgga aggctttgcc
     3061 gagaggctcg gcgcgcgcat cgaccatcgc ccgattttgc tcggcggagt gctcaagtcg
     3121 ctcgcgtcga cgccggcagc tggtccgccc gccaaacagg cgatgacgcg cgtggatgtg
     3181 gcgcgctggg cccagcactt tggcgtcgag ctgcacttcc ccgccgagca tccgcgacga
     3241 accgtcgaag cgatgcgact tttgacctgg gccccccggc tcagcatccc gtcgctcatg
     3301 gcggcacttt atcgcgccta ttgggtcgaa ggccgcgaca ttgccgatgt cgaggtgttg
     3361 ctggacatcg ctgaatcggt cggccttttg cgcagcgaag cccaaaaagg actgctaagc
     3421 ccagcagtgt cgcaggaact gagacgccag accgacgctg caattgccga tggagtgttc
     3481 ggtgttccga cgttcatcgt cgaaggggaa agcggtccgc ggctgttttt cggacaagat
     3541 cgtctgatct ttgtcgaaga agcgctgcgc agtcatccgc tgatgcgcga cgaaggctcg
     3601 acccggcccc cgcgtgcggt agcgccgact ccgattggcg agtacggcgc ccaagtcgcc
     3661 aatcaggcac gcaagctgac gtttgtgtat gacctgtcga gcccgtttgc gtatctggca
     3721 tccacccagg tggaaaaact ggcggcggcc tgcggagcag aagtcgagtt cttccccatc
     3781 ctgcttggcg gcctgttccg cagcatcggg acaccgatgg ttccgatttc gacctatccg
     3841 gaaagcaagc ggcggcacgc gctggaggac atgagtcgct gggcccatca ctatggcgtc
     3901 tcgtttcatt tcccgtcgcg ttttccgata aacacggtag cggcgctgcg gcttctgctt
     3961 ttggctgggg acaacattgc gccgctcacg catacgctgt ttcggctgta ctgggtcggg
     4021 gatcaagacc tgggcgatgt gcaagtcctg gagcgggcgc tttccgaggt cggactcgat
     4081 ccgcggctta tcgaccgcgt cggtgagccg gaggtcaaag ccaagctgct tgccaacacc
     4141 aaacaagccg aagagtgggg ctgtttcggc gtgccgtcat tcctcgtcgc ccagcatcac
     4201 ggcaaagccg agctgttttt cggccaagat cgcctgctgt tcgtcgaaaa agccctgcgc
     4261 cgcggcacgt cgcactgatg cacggacatg tcgcgccgac actgcgccag acccaggtcg
     4321 cgcggcatcc cacaaggcgt ccgacgaagc ttaggtggct ttcggctctt tgacgtaaaa
     4381 gagcgtgatc gtcagacagt ggaactcgcg gtccgagctc tgagtgacca ccttgtcgac
     4441 gacgcgcaac gacggattgc tgcgcaacca acgggagatg ttttcgccta gctcctctct
     4501 ctcgcgagct ttggtcgccg aaaagacctt cacgccggtg tacaccatct cccaagctcc
     4561 ttcaccctgc ggcaccgcgt attttgtgac ggtgccgggg ttggaaaagg taccgaagta
     4621 gcctaactag atatcatagt tctctcgcag caaaaggccg aaaaatcgtc tcagctgcat
     4681 gtggcccgta tcgttgaccg aaagagagac gcgccgctat gatggcgccg cttttttcac
     4741 cctgactgtc gctgcacggc ttgttttccg acggcgaaat atttgcgaaa acgccgtggc
     4801 gacgacgaaa agaggaacaa tatgtacgtg acgagcaaag atctgtggtt tgtcgctgca
     4861 aagcggacgc catttggcac ctacggtgga acgctgaagg acttcacggc gacggatttg
     4921 gcagtgcatg cagccaaagc cgcgattgcc gcaggcggtg tcgccacggc agatatcgat
     4981 caagtgatct tcggcaacgt gcagcagagc tcggcggacg cgctgtacct ggcccgtcac
     5041 attggtctgc gatcgggcgt gccggtgccg gcgccagcgc tgacgctcaa ccggctgtgt
     5101 ggatcgggtt tccaggcggt gatctcggcg gcagaacaaa ttcttcttgg ttccgcgacg
     5161 acggtgttgt gcggcggcac ggaatcgatg tcgcaagcgc cacacgttct gcgcggcgca
     5221 cgctggggtt ggccgctcgg caagccgccg gcaatcagcg acacgctgtg ggacgcgctg
     5281 accgatacgt ttaccggcat gccgatggcg atgaccgccg aaaagctcgc cgagcagtac
     5341 aagatcagcc gcaccgagtg tgacgagttt gcgctgacct cgcagaagcg ctggggcgaa
     5401 gcgcaggcga gcggctgttt cgaagcggag ttggcgccca tcgagatcac cagcaaaaag
     5461 ggcacggtgc gctttgccca agacgaacac ccgcgtccac agacgacgat cgagggactg
     5521 gccaagctgc cgacggtgtt caaaaaagac ggcgtgatca ccgcaggcaa cgcttcgggc
     5581 atctgcgacg gtgcggcagc gctgattctg accacccgcg aacacgccga gtcgaagggt
     5641 tataagccgc tcggacgact gatcggctgg ggaatttcgg gctgtgaccc gacggtgatg
     5701 ggcataggtc cagtgccggc gattcgcaac ctgctggcgt cgacgcgcac gcaggtttcg
     5761 gactacgacc tgttcgatgt aaatgaagcg tttgcacctc agttcctcgc ggtcgccaaa
     5821 gagctcggtc tgccgcttgc gcagagcaac gtctgcggtg gcgccattgc gctgggtcat
     5881 ccgctcgggg cgtcgggcgc gcgcatcacc gccaacctgc tccatgaact gcgtcggcgc
     5941 ggcggcaagc gcgcgatcgg cggcgcctgc atcggtggcg gccaaggcat cgccatcgcc
     6001 atcgaaagcg tctgaccaag gtcaaggaag aggcccacat gacccagcaa gccgcctcag
     6061 acatcatcta cgactggaac ttgcacgata cgcacgcgcc actgtggcca gcgaagtttt
     6121 cgctgttcga cgaaacgctg cgcgacggac tccaatcgcc atcggtccac gatccgagca
     6181 ttgaggacaa gattcagctt gtgctgctgc tcgatcgaat cggcgtcgag tatgtggaca
     6241 ttgcgctgcc cggcgcgggt ccacgagcag tcgaagatgg tctgcggctg gcctgcgaga
     6301 ttcgcgatcg caagctgcga atccgtcccg cctgcgccgc gcgcacgcac ctgaacgacg
     6361 tccgtccgat cgtcgaaatt tcgcaaaaag ccggcatcgc gatcgaggtc atgacgttca
     6421 tcggcagctc gccgattcgc cagtttgcgg aaaactggga caaagaacgc atcctcaaga
     6481 tgtcggcgga agcggtgtcg ttcgcagcgc aaaacaacct gccctgcact tacgtcaccg
     6541 aagacaccat ccgatcgcga cccgaaatgc tggcgccgct gttcaaaaac gccatcgact
     6601 cgggcgcgca caggctgtgt ctgtgcgata cggtcgggca cgcaacaccg acgggcgttg
     6661 acagcttggt gcgcttcgcc cggtcggtga tcgttggaat ggggctcgac gggaaaattg
     6721 gcatcgactg gcacggacac aacgatcgcg gtctcgccgt catcaattcg ctgtcggcac
     6781 ttcgtgcggg cgcagatcgc atccatggca cggtgctcgg cgtcggagaa cgagtcggca
     6841 acgccgcgct cgatcagctg ttggttaacc tgtatctgat gggcgcgctg ccagacgatc
     6901 gggatctgtc gtcgctggat gcgctgtgta cgctgatttc gcgcgcctgt caggtgccga
     6961 ttccgtacaa ctatccggtg ttcggcaccg atgcattccg gacagcgaca ggcgtacacg
     7021 cggcggcgat catcaaggcc gagcgcaaag gcgatgcatt tttggccgac aggatctatt
     7081 cgggcgttcc tgccgcgatg tttggtcgtc ggcaagagat cgaaatcggg ccgatgtcag
     7141 gcgaatcgaa tgtggcctac tggctacagc gcaacgggat cgcggcgtcg caagacgcgg
     7201 taaagcgcgt gttcgcaaaa gcaaagtcgg ccaatcgact gctctcgact gccgagattc
     7261 ttgcggtggt acaagcgacg gattgccaat cgacttgacg gcgcgtccct ccccgcggtc
     7321 tagtgaaata aagtgccttt tgccgcaact tctgcggccc aaaaccaccg ggctatcagg
     7381 cgaaccggcg acaccgggga gcaggcgaat gagcgacaag aacaagttca acggtgtgaa
     7441 ggtcttctca gcgacgatgg cgcaggaacg cgatcagctc ggcgaaaaag tgaccgcgtg
     7501 gctggcagaa cgacctgagc tccgcgtcgt cgacaccatc gtcacgcagt cgagcgacga
     7561 agcgttccac tgtctggcca tcacgctgtt ttaccaagac gatcgcgtct agcaccggac
     7621 ccctccatcc tgtgcgcccg ccccggcgtt gtgatctttt gcacagcgcc ttctgccgac
     7681 ctaaaccccg gatagaaaag ctttgtctga ctaggccgcg tcgtgggaca ctgcgaccat
     7741 ggccggtgtg taagcgcgag gcacgccggg ctccgttgcc atagctcatg attggccagg
     7801 tcgtcggaaa ctatcgcttg gtgcaaaagg tcggggaagg cggcatgggc gccgtctacg
     7861 aggccgtccg cgaagacatc ggcagccgcg ccgcgatcaa agtcctgcac aaagagctag
     7921 ccctaaaccc cgaaatggcc acccgcttct tcaacgaa
//
LOCUS       DAORGN010000219         7763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4296_length_7763_cov_5.346523, whole genome shotgun sequence.
ACCESSION   DAORGN010000219 DAORGN010000000
VERSION     DAORGN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_7763_cov_5.346523"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKL17_18360"
     CDS             complement(<1..614)
                     /locus_tag="PKL17_18360"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNK46753.1"
                     /translation="MSETTSALSSLQAILHAVAQTLPCNSASLALLEEDRHVLRMAVA
                     VNPRSAGDLLQVEHALGFQVGGLHIPLKLERSLLVRALREERVLLTTDVADLAGGALP
                     SEILDAVHTIAGPRSFAVVPVLGRIRPLGVMLVDRPGNSGFTGHERDLLISYAERVGA
                     ELESDALQNTALRLEQLGALAVPPPILWTCELSEGEGQLICHDAP"
     gene            complement(650..1735)
                     /locus_tag="PKL17_18365"
     CDS             complement(650..1735)
                     /locus_tag="PKL17_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46754.1"
                     /translation="MADLAQSTLPPFYAQRLGPERAQAAAQLRKLFADVGLHKQLWPR
                     WVDARPGLLAAIWVDLDDEPPLGQLGEALSTRYGPLLQDGAAALKLACAVPQVGFCSS
                     SDTRIADLRKLCGQTTLVVQKTPAVFPSLPEVDLAPEAGRSLVLSALVVAQVGALVQG
                     LAPLRLCSVVGAVNQPLVLDLNQPSASASGEWTPRDLVRLCAGSMHAAWVAIVGGALK
                     GTVWGADEPLPADADHLLILPAQHALLRRVRLLRSPEARIANACLGCDLCSSFCPDDL
                     LPHQAMRSFGPKASGPALSGCTGCGSCSVVCPAELLPSQLLFDRLGNTESQRRETETP
                     PPIRRGRLRIPTELMLSRLGLLQYQRR"
     gene            1882..3723
                     /locus_tag="PKL17_18370"
     CDS             1882..3723
                     /locus_tag="PKL17_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46755.1"
                     /translation="MMPRRFLLVLSLLAGCDGLWSGLREPVPENCVQNPSACSDGQVC
                     NAVTELCEAAGGALDLAMTDGVAGPCRPTDFCWQSPLPTGNNLRAVSMLSSTSGFAVG
                     ELGGLWQLSAAGWSAFRTTFKDTLLGVAAISDNQAVAVGQNGLVLRFDGNQWSKTSCN
                     TAVTLRSAYAMSPSDVWIVGDQGTVLRLAGSTCTPLTAGTALTLYAVRGIDAANVWIV
                     GQNGTVLFYDGQGFRSQMSGTKEDLLSLHVLSTTEAYASGTRGTVLKFAGGSWTGISL
                     GTTLSVDAVGGRTGQMLAATSNGAAYRFEAGGWKLFPTGSTYRLTGISASATDTWLVG
                     DYGTILSFDGQNVTRRTGNIETFNGILNIGSDSWVVGELGGTYRYQGGGWQPVASGVN
                     LPLWSLWGSSQTDVWAVGPAGSVIHWDGVSWQPRTAPVPSPRGISGTAANNIWMVGST
                     GNISRYDGTSWSKEPSGTGADLYAVWAFDASNVWAVGTSGSVVRRSGGKWQAEVAPTT
                     LGLLGVWAADANTVFAVGEQGTLLRRSANGWIKEQSGTSASLWGVMGSSAAAVWVVGT
                     GGQILENSSGAWKSVPSGTTNDLFQLAVRGSQVLVVGAGGTVISKSQ"
     gene            complement(3707..4552)
                     /locus_tag="PKL17_18375"
     CDS             complement(3707..4552)
                     /locus_tag="PKL17_18375"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46756.1"
                     /translation="MLIPHGASLLRIDWLATGERSLVTGPGRLGLSACPGRPDLGGTV
                     EDDVAKLRSLGVDVAVTLVDDRELEFYGVFGLRGALRKAGLQNIHFPMRDAEPPTDLL
                     ATRALCQELLRWLGEGKEVLIHCIGGWGRSGTIAASLLIHEGHTAQDAISQVRKARSP
                     RCVESTAQERFVEKYAEDHKHLRRFFVVLPQRLTDDRIGGQPGNRRLRRGARPPLAIC
                     EASGLRSQLAQLQDPTQARIVSGEALPEQLHSDVDLAIDRGFVQKDGGWRAVKLADLL
                     ATAIC"
     gene            4632..6482
                     /locus_tag="PKL17_18380"
     CDS             4632..6482
                     /locus_tag="PKL17_18380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNK46757.1"
                     /translation="MPSATSSSDILTLPFVPAYESSETATPVPAPKPAASGELLTRAL
                     SLLCPLPQALPKRDENAPIPIGDSSDDSAAAIASLRLVLGLLADHGYQHVALCLLADE
                     SEALTVWVSDAPGESVISARRLPILALRALFSRAAMAQLAEPDAVAQLAAVFGLPAGS
                     SSVMWACLVPPPPHGRDLGRFALILATDTDSEPTKNDIEALTPLLQVLALTLPGLRLD
                     ESVRERTRPLRVELVAPPVRSPGLGRYREFFESSTDGIIVLDGDGQVVWLNRVAEQLT
                     GYATAGLFGRPLSHYVPEAQREKVGKILSEVHLGQTPSNFDLPLTSTSQETLTLSAST
                     SQIAEPQRLCVVTFRDVTERRQLEAQLRRAKEFLDRVIDSTVDGIVVSDLRGNIRIFN
                     QGASRITGYCPDEVIGRLRVWRLFPEREAVHIMAELRSSDFGGPGRLLQARRIIVGKG
                     GERIPVALTAAMIRDGERELATVGIFQDLRERLQMEESLRNAKQQLEQTQKQAVLVEL
                     AGTTAHELNQPLLSVMGYAGLLKQKLPTEYVELHRFADVLLHESERMADIVRKIGRIT
                     LYETQSYVGETRILDLNKSSGEPAPLPPEPPPRPISDGPLFSRVRRQNPS"
     gene            6540..7298
                     /locus_tag="PKL17_18385"
     CDS             6540..7298
                     /locus_tag="PKL17_18385"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HNK46758.1"
                     /translation="MSLSVSNKTLLLLPVLWLVGCTDPAPETQMEPPPVPPSPDLSLV
                     QDMSQPLGPDLLARCELPYDVRNIDKVSTGTVTVMTAPSDPMVFTAEVDATAGGSMKY
                     GENPFVYLDLIGRKKVDITDVQSPNQGGWDIALKRWQLKINSGDSGNGGVTVAIVDGK
                     NLADVTKPPAGPYEADAYFDGKCVFQADPIGGLGTALSDWYDYESGTSRIIPKKEVIV
                     LKRRDGQGYIKLQILSYYKGSTSANYSLTWSYLP"
     gene            7295..>7763
                     /locus_tag="PKL17_18390"
     CDS             7295..>7763
                     /locus_tag="PKL17_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46759.1"
                     /translation="MSRRLCVLFLCSGLVGCGSDAAWQVPDPDGERVATLAQKIRPTD
                     GTGWNNNGFGVAADVYDPPGGHFRVFYVTSTDNAVDPTDVLPKNGVPDFVEAVGAAAE
                     ATYQSTVLARGFRQMLDDSVYHDRPDFGGDGRFDIYLRWAGKGSDGYRVTEACT"
BASE COUNT         1466 a         2321 c         2543 g         1433 t
ORIGIN      
        1 ggagcgtcgt ggcagatgag ctgaccttcg ccttctgaaa gctcacatgt ccacagaatc
       61 ggcggcggca ccgccagcgc acccagttgt tccagccgga gcgcggtgtt ttgcagtgca
      121 tccgattcca gctcggcccc gacgcgctcg gcataggaaa tcagcagatc gcgttcgtgt
      181 cctgtaaagc cgctgtttcc gggccggtcg acgagcatca ccccgagcgg gcgaatccga
      241 ccgagcactg gcaccaccgc aaacgagcgc ggacccgcga tggtgtgcac cgcatcgagg
      301 atctccgacg gcagcgcgcc acccgctaga tcggcgacgt cggtggtcag cagcacgcgc
      361 tcttcacgca gcgcccggac cagcaggctg cgctcgagct tgagtggaat gtgcagtccg
      421 ccgacctgga accccaacgc atgctcgact tgcagcagat cgccggcgga gcgtggattg
      481 acggcgacgg ccatgcgcaa gacgtgccgg tcttcttcga gcagcgcgag gctggcgctg
      541 ttacacggca gggtctgggc gacggcgtgc aagatcgctt gtaacgacga aagcgcggac
      601 gttgtctcgg acacctgggg agaatcgtcg tgtcagggca cgctgtcaat cagcgacgct
      661 ggtattgcaa aagcccgagc cgtgacaaca tcagttccgt cggaatgcgc agccgtccac
      721 gacgaatcgg cggtggtgtt tctgtttcac gccgttggct ttcggtgttt cccagtcggt
      781 caaaaagaag ctgcgagggc agaagttccg ccggacagac caccgagcag gagccacagc
      841 cggtgcagcc cgaaagcgca ggtccagatg ccttgggtcc gaagctgcgc atcgcttggt
      901 ggggcaaaag atcgtcggga cagaagctgc tgcacaggtc acagccaagg caagcattgg
      961 caatgcgggc ttctggactt cgcagcagcc gaacgcgccg cagtagtgcg tgctgagcgg
     1021 gcaaaatcag cagatggtcc gcgtcggcgg gcagcggttc atctgcgccc cacacggttc
     1081 ctttgagcgc tccaccgacg atggcaaccc aggcggcgtg catgctgcca gcgcaaagtc
     1141 gaaccaagtc gcgcggtgtc cattccccgc tggcggatgc acttggctgg ttcaagtcga
     1201 gcaccagcgg ctgattcacc gcaccaacga cagaacacag acggagcggc gcaaggccct
     1261 gcacaagcgc acccacttgc gcgacgacaa gcgccgagag caccaagctg cggcccgctt
     1321 ccggggccag atcaacttcg ggcagcgacg ggaaaactgc gggcgtcttt tggaccacca
     1381 gcgtcgtttg gccgcacagc ttgcgcagat ccgcaattct ggtatcggac gacgaacaga
     1441 agcccacctg cgggacggca caggccagct tgagcgccgc cgcgccatct tgcaaaagcg
     1501 gcccatatcg ggtcgacagg gcttcgccga gctgaccgag cggcggttca tcgtccagat
     1561 cgacccaaat cgccgccaaa aggcctggcc gcgcatcgac ccagcgtggc cacagctgtt
     1621 tgtgaagacc gacgtcagca aacagcttgc ggagctgcgc cgcggcctgt gcgcgctcgg
     1681 gcccgagtcg ctgggcataa aacggcggca gggtgctttg cgcaaggtcg gccatgggcg
     1741 cggccatgct agcagaaacc gcaatgatcc cgcaggtcta cacggaaagc tggcgtcggc
     1801 cttgcgatcc gtcacactga aatcttggtg tacgaggcgg cgggcaacgc acagcgctct
     1861 tcacgactta tggagaccac cgtgatgccg agacgctttc tactcgtcct gagccttttg
     1921 gctggctgtg acggattgtg gtcgggcctg cgtgagccgg tgcccgaaaa ctgtgtacag
     1981 aatccgtctg cgtgcagcga tggtcaggtc tgtaacgccg tcaccgagct gtgtgaagcc
     2041 gcgggtgggg ctttggacct ggcgatgacc gatggtgtgg ctggaccgtg tcgtccgacg
     2101 gatttttgtt ggcaaagtcc gctgccgact ggcaacaacc tccgcgcggt gtcgatgctg
     2161 tcgtcgacga gcggttttgc cgttggggaa ctcggcgggc tttggcagct ttcggcggcg
     2221 ggctggtcgg cgttccggac gacgttcaaa gacacgctgc tcggcgtggc ggcgatttcc
     2281 gacaaccaag cggtggccgt cggtcaaaac ggtctcgtgc tgcgttttga cggcaatcag
     2341 tggtccaaaa caagctgcaa taccgcggtg acgctgcgca gcgcgtatgc gatgtccccg
     2401 tcggatgttt ggatcgtcgg tgaccaaggt acggtgcttc ggctcgctgg ttcgacgtgc
     2461 acgccgctga ccgcagggac cgcgctgacg ctttacgccg tccgcggcat cgatgcggcc
     2521 aatgtttgga tcgtcggcca gaacggcacg gtgctttttt acgacggaca gggcttccgc
     2581 tcccagatgt ccggcaccaa agaagacctg ctcagcctgc acgtcttgtc gacgaccgag
     2641 gcctatgcca gcggcacccg cggcaccgtg ctcaagtttg ccggcggcag ctggactggg
     2701 atcagtctgg gcaccacgct gtccgtcgat gcagtcggtg ggcgaacggg tcagatgttg
     2761 gccgcgacga gcaacggcgc tgcctatcgg ttcgaagcgg gcggatggaa gttgtttccg
     2821 accggcagca cctatcgcct gaccggaatc agcgcgtctg cgaccgacac ctggctcgtc
     2881 ggcgactacg gaaccattct gtcgttcgat ggccagaacg tgacgcgccg caccggcaac
     2941 atcgaaacgt ttaacggcat cctcaacatc ggttcggact cgtgggtcgt cggtgaattg
     3001 ggtggaacct atcgctatca aggcggcggc tggcagccgg tggcgtcggg cgtgaacttg
     3061 ccgctgtggt cgctgtgggg tagctcgcag acggacgttt gggccgtcgg tccggctgga
     3121 tcggtgattc actgggacgg cgtgtcgtgg cagccgcgaa ccgcgccggt gccatctccg
     3181 cgcggcataa gtgggacggc ggcaaacaac atctggatgg tcggcagcac cggcaacatc
     3241 agtcgctacg acggcacaag ctggagcaaa gaaccgagtg gcacgggcgc ggatctttac
     3301 gcggtgtggg ctttcgacgc gagcaacgtc tgggcggtcg gcaccagcgg ttccgtcgtc
     3361 aggcgcagcg gcggcaagtg gcaagcggaa gtagcgccga cgacgctggg acttctgggc
     3421 gtttgggctg cggatgcaaa caccgtgttt gccgttggag aacaaggaac gctgttgcga
     3481 cgctcggcga acggctggat aaaagaacaa agtggcacca gcgccagcct gtggggcgtg
     3541 atgggatcgt cggcagctgc ggtctgggtg gtgggaaccg gcggacagat tttggaaaac
     3601 agcagcggcg cttggaagtc ggtgccgagc ggaacgacaa acgatctgtt ccagctcgcg
     3661 gtgcgtggca gtcaggtgct cgtcgtcggc gcgggtggaa ctgtgatcag caaatcgcag
     3721 tagccagcaa gtcggcaagc ttcaccgccc gccagccgcc gtctttttgg acaaagccgc
     3781 gatcgatcgc cagatcgaca tcgctgtgaa gctgctcggg cagcgcttcg cccgatacaa
     3841 tccgcgcctg cgtcgggtct tgtagctgcg caagctggct gcgcagtccc gacgcttcac
     3901 agatcgcaag tggtgggcgc gcacctcgtc gcagacggcg atttccaggc tgtcccccga
     3961 ttcgatcgtc ggtgagtctc tgcggcagca ccacaaaaaa acggcgcagg tgtttgtggt
     4021 cttcggcgta tttttcgacg aagcgttcct gggcggtcga ttcgacgcag cgcggactgc
     4081 gggctttgcg aacctgcgaa atcgcgtctt gcgcggtgtg gccttcgtgg atgagcagcg
     4141 acgccgcgat cgtgcccgaa cgaccccagc cgccgatgca gtggatcagc acttctttgc
     4201 cttcgccgag ccagcgcaac agttcctgac acagcgcccg cgtcgccagc agatcggtcg
     4261 gcggttcggc atcgcgcatc ggaaagtgga tgttttgcag accggctttt cgcagcgcgc
     4321 cgcgcagacc gaacacgccg taaaactcca gctcgcgatc gtcgaccagc gtcaccgcca
     4381 catcgacccc gagcgagcgc agcttggcca catcgtcttc gacggtgccc ccgaggtcag
     4441 gccgccccgg acacgccgac agaccgagtc gtcccggtcc ggtcacaagc gatcgttccc
     4501 ccgtggccag ccagtcgatg cgaagcagcg acgctccgtg cggaatcagc atgccgatta
     4561 ctttactctg tgacggtttg gtgacgcgag ttcgctgaca ctggctgcaa agcttgctaa
     4621 cctgcgccgc cgtgccgtcc gcaacgtcgt cgtccgacat tctgactctg ccgtttgttc
     4681 ccgcttacga gtcgagcgag accgcgacgc ctgtgcctgc gccaaaacct gcggcgagcg
     4741 gcgagctttt gacacgggcg ttgtcacttt tgtgtcccct tccgcaagcg ctgccaaagc
     4801 gcgacgaaaa tgcaccgatt ccgatcgggg acagcagcga cgacagcgcg gcggcgattg
     4861 cgagcttgcg gcttgtgctt ggactgcttg ccgaccacgg ctatcagcat gtggcgctgt
     4921 gtcttttggc ggacgaaagc gaagcgctga cggtgtgggt gagtgacgcg ccaggcgaat
     4981 cggtgatatc ggctcgacgg ctgccgattt tggccctgcg tgcgctgttt tcccgcgccg
     5041 cgatggcgca gcttgccgaa ccggatgcag tcgcgcagct tgccgcggtg tttggcctgc
     5101 ccgctgggtc gtcgtcggtg atgtgggcat gccttgtccc gccgccgccg catgggcgcg
     5161 atctgggccg ttttgcgctg atcctggcga cggatacaga ttcggaaccg acgaaaaacg
     5221 acatcgaagc gctgacgccg cttttgcaag tgctggcgct cacgctgccg gggctgcggc
     5281 ttgatgaaag tgtgcgcgaa cgaacccgac cgctgcgcgt cgagctggtt gcaccccccg
     5341 tgcgttctcc cggacttggc cgctatcgcg agttttttga atcgagcacc gacggaatca
     5401 tcgtgctgga tggcgacggc caggtggtgt ggttaaaccg cgtcgctgag cagctcaccg
     5461 ggtatgcgac ggcggggctt tttggccgtc ctctttccca ctatgttccc gaagcgcagc
     5521 gcgaaaaagt cggcaagatt ctgtcggaag tgcacctggg ccagacgccg tcgaactttg
     5581 atctgccgct gacttcgacg tcgcaggaaa cgctgacgct gtcggcatcg acgtcgcaga
     5641 ttgccgaacc gcaaaggctg tgcgtcgtca cgttccgcga tgtcaccgaa aggcgacagc
     5701 tggaagcgca gctgcggcgg gccaaagagt ttctcgaccg cgtgattgac tcgacggtgg
     5761 acggcatcgt ggtgtccgat ttgcgcggca acattcgcat tttcaaccag ggcgcaagcc
     5821 gcatcaccgg ctactgtccc gacgaagtga tcgggcggct gcgggtctgg cggctgtttc
     5881 ccgagcgcga agccgtgcac atcatggccg agctgcgctc gagcgacttt ggcgggccgg
     5941 gacggttgct gcaggcgcga cgcatcatcg ttggcaaagg cggcgagcgc atcccggtgg
     6001 cgctgacggc agcgatgatt cgcgacggtg agcgcgagct ggcgacggtc gggatttttc
     6061 aggatctgcg tgagcgcttg cagatggaag agagtctgcg caatgccaaa cagcagcttg
     6121 aacagacgca gaaacaggcg gtgctcgtcg agctggcggg caccacggcg cacgaactga
     6181 accagccgct tctatcggtg atgggctatg cggggctttt gaaacagaag ctgccgacgg
     6241 aatatgtcga actgcaccgc tttgccgacg tgctgttgca tgaatcggag cgcatggccg
     6301 acattgtgcg caaaatcgga cggatcacgc tctacgagac ccagtcctac gtgggcgaga
     6361 cgcgaatttt ggatctgaac aagtcgtcgg gcgagccagc accgctgcct ccggagccgc
     6421 caccgcgacc tatcagcgac gggcctctgt tttcgcgggt acggcggcag aatccgtcgt
     6481 aaaaacagcg caaattggcc caaaaccgct aaaaacaagc acttttgagg agccttgcga
     6541 tgtcgctttc cgtgtcaaac aagacgctgt tgcttttgcc tgtgctgtgg cttgtcggtt
     6601 gtaccgatcc cgcccccgaa acccagatgg aaccgccgcc agtgccgccg tcgcctgacc
     6661 tttcgcttgt gcaggacatg agccagcctt tgggaccgga tctgttggcg cgctgcgagc
     6721 tgccctacga cgtgcgcaac attgacaaag tgtcgacggg aacggtgacg gtgatgaccg
     6781 cgccgagcga tccgatggtg tttaccgcgg aagtcgatgc gacggcgggc ggatcgatga
     6841 aatatggcga aaatccgttt gtttatctgg acctgattgg gcgcaaaaaa gtcgacatca
     6901 ccgacgtgca gtcgccaaat caaggtggct gggacattgc gctcaagcgc tggcagctaa
     6961 aaatcaacag cggcgattcc ggaaatggcg gcgtcaccgt ggcgattgtc gacggaaaaa
     7021 acctggcgga cgtgaccaaa ccgccggctg gaccgtatga agcggacgcc tatttcgacg
     7081 gaaaatgcgt gttccaagcc gatccgattg gggggctggg aactgcgctg tccgactggt
     7141 acgactacga aagtgggaca agccgcatca ttcccaaaaa agaagtcatc gtgctgaagc
     7201 gccgcgacgg gcagggctac atcaagctgc aaatcttgag ctactacaaa ggctcgacga
     7261 gcgcaaacta cagcctgacg tggagttacc tgccgtgagc cgacggctgt gcgttttgtt
     7321 tttgtgcagc ggtcttgtcg gttgtgggtc agacgcagcg tggcaagtgc cagatcccga
     7381 cggcgagcgc gttgctacgc tggcgcaaaa aattcgcccc accgacggaa cgggctggaa
     7441 taacaacggc tttggtgtgg ccgcagacgt ctacgatcct cctggcggtc actttcgcgt
     7501 tttttacgtc accagcaccg acaacgctgt cgatcccacc gacgtgctgc cgaaaaatgg
     7561 cgtacctgac tttgtcgaag ccgtcggagc cgcggcggaa gcgacgtatc aaagcacggt
     7621 cttggcgcgc ggttttcgtc aaatgctcga cgacagcgtc tatcacgacc gaccggattt
     7681 tggcggcgac ggacggtttg acatctatct gcgctgggcc ggcaagggca gcgacgggta
     7741 tcgcgtcacc gaagcctgca ccg
//
LOCUS       DAORGN010000220         7760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4299_length_7760_cov_5.995068, whole genome shotgun sequence.
ACCESSION   DAORGN010000220 DAORGN010000000
VERSION     DAORGN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_7760_cov_5.995068"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKL17_18395"
     CDS             <1..943
                     /locus_tag="PKL17_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46760.1"
                     /translation="SGAGGTAYGQANKPAAAAQPAAAPAADPPAAEPPAAEPPAAALP
                     PTGETTTSEVSGAKTPTDEVKAKTRWDDIAVLPRRYTLKARRLELSPQYHFTLNNPLI
                     RHHAVGAQINYFLSEALWLGVEGQYYFPQQSSDVLGTYFLIGAQDKALPAVNKLIFSA
                     LLDFGYVPVAGKFALFNRAIVHWEGYVTLGVGAFMSEVIPVKRSDPAFRTISAIFDIG
                     VGSRLFLTRWLALNAFLKLYGYPDKFEPVANASFTRSGAGCSSYLTGQSAGSQAFEDA
                     YTQCRKDNGSTTLVMDVQFGLGLSIFLPPKFEYKMAR"
     gene            961..1884
                     /locus_tag="PKL17_18400"
     CDS             961..1884
                     /locus_tag="PKL17_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46761.1"
                     /translation="MRTHMRIILSFGALALLAAPAHADRGRNPLAGQPAIRHRVEMRK
                     MRLEITPQFTISANQPFLIGFGGGANVRFHLTDWLAIGGSYHYTANIASPLVGRITEA
                     LPDTYAVRTSSPATDDPTAGLRQPSKDIFKDHLIGPTLGMWSAHVALTPMGGKFSLFN
                     ALFANYDFYGTVGIGGVILGTPLASGASSYTVRTDSSGTTSVRPVNDTAQTSANDVNL
                     QSPSVFTGHRIAGVFGIGTHIFFNHFIGLNLELRDYMYMANPGGLDVKTTDSNSDGSG
                     VLSSQDEYLVNHLYFGVGLTLMLPPAAKISR"
     gene            1987..3468
                     /locus_tag="PKL17_18405"
     CDS             1987..3468
                     /locus_tag="PKL17_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNK46762.1"
                     /translation="MSVAPSPVPHLKPFVGGRFIETATIRELRAPYDGRLLALVSECG
                     PSEIDAALGHAEAAAQTTRRITTDQRAAICRAIASGLGADAERLAGAICDDAGKPIGD
                     ARAEVQRAILCFELAAAEALTTSGEGQVLPMDLAPLGYGRTGILRRFPIGPIAAISPF
                     NFPLNLAVHKVAPAIAAGCPIVLKPASQTPTVALLLAEIVARAGWPSTALQVVPAARA
                     AADRLVTDDRCKLLTFTGSASVGWDMKARAGKKKVTLELGGNAAVVIDDTISEADLCA
                     IIPKLVYGAYSYSGQKCISIQRIYVVAQKGDRLYRAVCEKLAAATAAIRTGDPRDESV
                     LVGPMIDESNARRVDSWIAEAEQLGARKLVGGSRQGTLVPPTLLADVPPEAKVVREEV
                     FGPVCNVDQVPSFEAALAAVNDSRFGLQAAVFTHDLGHALLAHEALEVGAVILNEAPS
                     FRVDHMPYGGVKDSGLGREGIRTAIVDMTEPRLLITGALSPSH"
     gene            3488..4357
                     /gene="folD"
                     /locus_tag="PKL17_18410"
     CDS             3488..4357
                     /gene="folD"
                     /locus_tag="PKL17_18410"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNK46763.1"
                     /translation="MTEKARILDGKAISQKVRDEVRSEVTALAQKGIRPGLAVVLVGD
                     DPASQVYVRNKGRACHEVGITVFDHHLPTQTTKADLLALILRLNADPAVHGILLQLPI
                     PATLPTSEILDSIDPQKDVDGLLEANVGRLWLSRPRFVPCTPLGVMRILAEAGTKLAG
                     ARALVVGRSILVGRPMAALLLAADASVTICHSKSLDLFDRVAESDVVVAAIGKPQAIP
                     GRYIKPGATVIDVGINRTEAGHLVGDVEFSVAVERASAITPVPGGVGPLTIAMLLSNT
                     VLAAKLRHGLVTA"
     gene            complement(4354..5640)
                     /locus_tag="PKL17_18415"
     CDS             complement(4354..5640)
                     /locus_tag="PKL17_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNK46764.1"
                     /translation="MRIFLFVLSLWLGLSMACGRAAAPPPAAPAPSGKNVVLVLIDGI
                     RQQEWGGRAVDDAGRPVKMADMFPVLLQLRKRGLWLPNVAISNPAGVSLPAYSDIFAG
                     RRQEKILSNYPPAEDFHSHYPTLMQEARKQLRLGFDGVSLIASWGPLCTIAFQPPLIP
                     DDDFYRSCAFKVNASPGPEQPLSFKPEVYSGSRTDVDTFMEFLREVPKRHPRLLVIHL
                     GDADEEAHLHARVFRKTGQHYGIFHYHQALRQDDYLVGRIWDALQADPFYRDNTYLIV
                     TTDHGRDTAEDPAQWASHGRCIAEKIRTKPCSGCSGVFALAVGPGIPARTARGSYLHT
                     DIAPTIAKLLNIEFPSATGKPIREITDPASRVPAQHSPMTTPAPFLTISPTPPGSPAA
                     AAIKAQAAVSAQSSSGSPAPPSPSTNPVPPAPPVAR"
     gene            5735..6655
                     /locus_tag="PKL17_18420"
     CDS             5735..6655
                     /locus_tag="PKL17_18420"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNK46765.1"
                     /translation="MSNFSFAIISDIHVYTSTGSSPGAGIPSNFSKVIAQLASLTPRF
                     VVVSGDATSGNPDDGASRSKVDGWWRGFREALRPLTESGIPVLPIAGNHDHYTQAQRD
                     GYQAAWNLLSEDVSSQFVLWGKPPLCYSLQVDELHLSLLHVIDQDLPSEIAKFLRADA
                     EQTPVDALRLCIGHVPLVSMMGRTSESYRDELGKLLVSLGYSAFFAGHEHLVWEQELS
                     FPEGNLHHIHVGTASGTYNFPLSRSTYEKHCSGNQGTLPYSGQRFALDPGTRQQADKT
                     NVCVVQVSDGTYSVTHLALRGGELQPFGIP"
     gene            complement(6659..7486)
                     /locus_tag="PKL17_18425"
     CDS             complement(6659..7486)
                     /locus_tag="PKL17_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46766.1"
                     /translation="MGDSLRVYRDLAVACLLAASACAGPRAETSSGGGSGKLPPQVLD
                     TSDVQELIGSLSGSSPRCQNGFCTCREIDAAGRGAAETDAESSPPAPGFKRFELRTGR
                     GDDTMRITIEGVGTFVKIGPSPEARCIYVDLKPGAYRVRYHVTAANPTQGAEPRLRIS
                     EYGEKWKRWYRTFGFRCTDGSQPCSKDLAKDEFERLTKQSDGKFDPCGSTKVQGMRFS
                     TDREADASVGDFNLQLVLSVYKFAPRFAPDTANCKGLSPTKSQSSDDEGEMTVQSAQ"
     gene            complement(7538..>7760)
                     /locus_tag="PKL17_18430"
     CDS             complement(7538..>7760)
                     /locus_tag="PKL17_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNK46767.1"
                     /translation="GAVRRRVFLQMGGFDAERARVALEVPEQFAVNVMMAIGHPGPVE
                     ELPERLREREVQSARRPVQELIFKGKFAR"
BASE COUNT         1488 a         2448 c         2358 g         1466 t
ORIGIN      
        1 ctctggcgca ggcggaacgg catacgggca ggcgaacaag ccggccgcag cagcacaacc
       61 ggcagcagcc ccggcggcgg acccgccagc ggcagagcca ccggctgcgg aaccaccagc
      121 cgccgccctg ccaccgacgg gagagaccac cacatcagaa gtttcgggag ccaaaacccc
      181 gactgatgag gtgaaagcga agacgcggtg ggacgacatc gcagttcttc cccgtcgtta
      241 cacactcaaa gcgcgacgat tggaactgtc tccgcagtac cacttcacgc tcaacaaccc
      301 gctgattcgg caccacgcgg tcggcgctca gatcaattac ttcctgagcg aagccctgtg
      361 gctcggtgtc gagggtcagt actatttccc gcagcaaagc tctgacgtgt tggggaccta
      421 cttcctcatc ggtgcgcagg acaaagccct gccggcggtg aacaagttga tcttctcggc
      481 attgcttgac ttcggctacg tccccgtcgc tggcaagttc gcactgttta accgggcgat
      541 cgttcactgg gaaggctacg tcactcttgg cgtcggcgct ttcatgagcg aagtgattcc
      601 ggtcaagcgg tcggacccgg cgttccggac gatcagtgcc atcttcgaca tcggtgtggg
      661 ttcgcgcctg ttcctgactc gatggctggc gctcaacgcg ttcctcaagc tgtacggtta
      721 tcccgacaag tttgagccgg tcgcgaacgc ttcgtttacc cgaagcggag cgggttgcag
      781 ctcgtatctg acgggccagt cggcaggaag ccaggcgttt gaagacgcat acacccagtg
      841 ccgcaaggac aacggcagca ccaccctggt gatggacgtg cagttcggtc tgggcttgtc
      901 gatcttcctg ccgccgaagt ttgaatacaa gatggctcgg taagaggagc gagggattac
      961 atgcgaacgc acatgcgaat catcttgtcg tttggggccc tcgcgctgct ggcagcgcca
     1021 gcgcatgcag accgtgggcg caacccgctg gcgggtcagc cggcgatccg gcaccgcgtc
     1081 gagatgcgca agatgcggct cgaaattacg ccgcagttta cgatctcagc gaaccagccg
     1141 ttcctgattg gcttcggtgg tggtgcgaac gtccgcttcc acctgaccga ctggctggcg
     1201 attggtggct cgtatcacta caccgccaac atcgcttcgc cgctcgtcgg tcgtatcacc
     1261 gaagcgctgc cggataccta cgcggtccgc accagctcac ctgccacgga cgatccgacc
     1321 gccggtctgc gtcagccgag caaggacatc ttcaaggatc acttgatcgg tccgacgctg
     1381 ggcatgtggt cagcgcacgt cgccctgacc ccgatgggtg gcaagttctc gctgtttaac
     1441 gcgctgtttg ccaactacga cttctacgga acagttggta tcggcggcgt gatcctcgga
     1501 acgccactgg cgagcggcgc gtcgtcgtac acggttcgca ccgatagctc gggaacgacg
     1561 tcggttcgtc cagtgaatga cacggcgcag acgtcggcca acgatgtcaa cttgcagtca
     1621 ccgtcggtgt tcaccggcca ccgcatcgcg ggcgtgtttg gcatcggcac gcacatcttc
     1681 ttcaaccact tcatcgggct caacctcgag ctgcgggact acatgtacat ggccaaccca
     1741 ggcggcctgg acgtgaagac caccgacagc aacagcgatg gctcgggcgt cctgtcgagc
     1801 caggatgagt atctggtcaa ccacctgtac ttcggggtcg gtctgacgct gatgctgccg
     1861 cctgcggcga agatttcgcg ctaaccaaag cctgttgctt ttgaaagagc ccaaggggat
     1921 ttcctcttgg gctttttctt tttccgaagg cgtgactcct cgccgaagtt gacgcatctt
     1981 gttcgcatgt cggtcgcgcc gtctcccgtc ccccacctga aaccatttgt tggcggtcga
     2041 ttcatcgaaa cggccaccat ccgcgagcta cgtgctccat acgacggacg cctgcttgcg
     2101 ttggtttccg aatgtggccc aagcgaaatc gacgccgcgc ttggccatgc cgaagccgca
     2161 gcccagacca cacgacggat caccaccgac cagcgcgctg cgatctgccg cgcgattgcc
     2221 agcggacttg gtgccgatgc agagcgactc gctggggcca tctgtgacga cgccggcaag
     2281 ccgattggcg atgctcgcgc cgaggtccag cgcgccatcc tctgctttga gctggccgcc
     2341 gccgaagcac tgacgacttc gggtgaaggc caggtgctgc ccatggatct ggcgccgctt
     2401 ggctacggac gcaccggaat tttgcgacgc tttccgatag gcccgatcgc ggcgatcagc
     2461 ccgttcaatt ttccgctgaa cctggcggtg cacaaagtgg cgcccgcgat cgccgccggc
     2521 tgcccaatcg tgctcaagcc agcctcgcag acaccgaccg tcgccctttt gctggccgag
     2581 atcgtcgcac gcgccggttg gccaagcacc gccctgcaag ttgttcccgc cgcacgcgcc
     2641 gccgccgatc ggctggtcac ggacgatcgc tgcaagctgc tcacgttcac gggctcggcg
     2701 tcagtcggct gggacatgaa agcgcgcgcc ggcaaaaaga aggtcacgct ggaactgggc
     2761 ggcaacgcgg cggttgttat cgacgacacg atcagcgaag ccgatctttg cgccatcatt
     2821 ccgaagcttg tctacggcgc ctattcgtat tccgggcaaa agtgcatcag catccagcgc
     2881 atctacgtcg tcgcacaaaa aggcgatcgg ctgtaccgcg cggtgtgtga aaaactggcc
     2941 gcagcaacgg cggcgattcg gaccggcgat ccacgcgatg agtccgtgct cgtcgggccg
     3001 atgattgatg aatcgaacgc ccgccgggta gattcgtgga ttgccgaagc ggaacagctc
     3061 ggagcgcgca agctcgtcgg tggatcgcgg caaggcacgt tggttccgcc gacgcttttg
     3121 gccgacgtgc cgcccgaggc caaagtggtt cgcgaagagg tctttggacc ggtctgcaac
     3181 gtcgatcagg tcccgagctt tgaggcggcg ctggctgcgg tcaatgactc gcggtttggc
     3241 cttcaggcgg cggtgtttac ccatgacctg gggcatgcgc tcttggccca cgaagcgctc
     3301 gaagtcggcg ccgtgatcct aaacgaagcg ccgagcttcc gcgtcgacca catgccctat
     3361 ggcggagtca aagactcggg gctgggacgc gaaggcatcc gcacggcgat cgtcgacatg
     3421 acggagccgc gcctgctcat cacgggcgca ctttccccat cgcactagaa agatgagtgt
     3481 caggtttatg acggaaaaag cccgcattct cgacggcaaa gcgatctccc aaaaagtccg
     3541 cgacgaggtc cgaagcgaag tgacggcgct tgcgcaaaaa ggcattcggc ccggcctcgc
     3601 ggttgtcctc gtcggagacg acccggcgtc acaagtctac gttcgcaaca aaggccgcgc
     3661 ctgccacgaa gtcggcatca cggtctttga ccaccacctg cccacccaga cgaccaaagc
     3721 cgatctactc gcgctgatct tgcggctcaa tgccgatcca gcggtgcacg gcatcctgct
     3781 gcagcttccg attccagcga cgctgccaac cagcgaaatc ctcgattcga tcgatccgca
     3841 gaaagacgtc gacgggctgc tggaagcaaa tgtcggtcga ttgtggcttt cgcgaccgcg
     3901 ttttgtgcca tgtacaccgc ttggcgtgat gcgaatcctg gccgaagcgg gcaccaagct
     3961 ggcgggagcg cgggcgctgg tcgtcgggcg ctcgatcctg gttggcagac cgatggcggc
     4021 gctgctgctg gcggcggatg catcggtgac gatctgtcac tcgaagagcc tcgacttgtt
     4081 tgatcgggtc gccgaatccg atgtcgttgt cgctgcgatc ggcaaaccac aggccatccc
     4141 cggacgctat atcaagcccg gtgcgacggt gatcgacgtc ggaatcaacc gcacagaagc
     4201 aggtcatttg gtcggtgatg tggaattttc cgttgcagtc gagcgtgcgt ctgccatcac
     4261 gcctgtcccc ggcggagtcg gtccgctgac gatcgcaatg ctgctttcca acaccgtgct
     4321 cgccgccaag ctacgccatg gtcttgtgac cgcttagcgc gcgacgggag gagccggcgg
     4381 aacagggttc gtcgacgggg atggaggcgc tgggctcccc gaagaggact gcgccgacac
     4441 ggcggcttgc gctttgattg cagcggcagc gggacttccc ggcggcgtcg gagaaatcgt
     4501 caaaaagggc gctggcgtcg tcatcggact gtgttgcgcc ggcacgcgcg aggccggatc
     4561 ggtgatctcg cgaattggct tgcccgtcgc actcgggaac tcgatgttga gtagcttggc
     4621 gatcgtcggc gcgatgtcgg tgtgcagata gctgccccgt gcggtccgcg ccggaattcc
     4681 tggtccgacg gccagagcaa agacgccact acagcccgag cagggcttgg tgcggatttt
     4741 ttcagcgatg cagcgcccgt gcgaagccca ctgcgccgga tcttcggcgg tgtcacgtcc
     4801 gtggtccgtc gtcacgatca ggtacgtatt gtctcggtaa aacgggtcgg cctgcagcgc
     4861 atcccagatc cgcccgacca ggtagtcgtc ctgccgcagc gcttggtggt agtgaaaaat
     4921 gccgtagtgc tgcccggtct tgcgaaacac ccgcgcatgc aagtgggctt cctcatcggc
     4981 atcgcccagg tgaatcacga gcagtcgcgg atgccgcttg ggtacttcgc gcaaaaattc
     5041 catgaaggta tccacgtcgg tgcgcgagcc ggaatagacc tcgggcttaa acgacaaagg
     5101 ctgctctgga cctggcgaag cgttcacctt aaaagcacag cttcgataga aatcgtcgtc
     5161 gggaataagc ggcggctgaa acgcgatcgt gcacagcggt ccccacgacg cgataagcga
     5221 cacgccatca aatccgagcc gaagctgttt gcgcgcttcc tgcatcagcg tcgggtagtg
     5281 gctgtgaaaa tcctcggcgg gcggatagtt gctgagtatt ttttcctgcc gtcgccccgc
     5341 aaaaatgtcc gagtacgccg gcaagctcac gccggcggga tttgaaatgg cgacatttgg
     5401 cagccacagc cctcgcttgc gaagctgaag cagcaccgga aacatgtccg ccatcttgac
     5461 cggcctgccg gcatcatcga cggcgcggcc tccccattct tgttggcgaa ttccatcaat
     5521 cagcaccagc acgacgtttt ttcccgacgg tgccggtgcc gcaggcggag gcgctgccgc
     5581 ccgaccacac gccatgctca gccccagcca aaggctcagc acaaaaagaa atattcgcat
     5641 tttccattac atattacggg aagccaacta cccatgtcta ggcggcgcag gaccacgctc
     5701 gaccgcaacc gacaaagcct gctagaacgt ggtgatgagc aatttcagct tcgcgatcat
     5761 ctcggacatc cacgtctaca cgagcaccgg cagcagtccg ggcgccggta tcccgtcgaa
     5821 tttttccaaa gtgatcgcgc agctggcatc gctgacgccg cgatttgtgg tggtcagcgg
     5881 cgatgccacc agcggcaatc ccgacgatgg cgcgagccgc agcaaagtcg acggctggtg
     5941 gcgtggcttt cgcgaagcac tcaggccgct gacggaatca ggcatcccag tgctgccgat
     6001 cgccgggaac cacgaccact acacccaggc gcagcgcgac ggctaccagg cggcgtggaa
     6061 cctgctgagc gaagacgtca gcagccagtt tgttttgtgg ggcaaaccac cgctttgtta
     6121 ttcgctgcaa gtcgacgagc tgcacctatc gctgctgcat gtgatcgatc aggatctgcc
     6181 gagcgagatc gccaagttcc tccgcgccga cgctgagcag acccctgtcg atgcgctgcg
     6241 gctctgcatc ggccacgttc cgcttgtgtc gatgatgggt cgcacaagtg agtcgtaccg
     6301 cgacgagctt ggcaagctgc tcgtctcgct tggttactcg gcgttttttg ccgggcacga
     6361 gcacttggtc tgggaacaag agctgagctt tcccgaaggc aatctgcacc acattcacgt
     6421 cggcaccgcc agcggcacct acaactttcc gctgagccgc agcacctacg aaaaacactg
     6481 ctcgggcaat caaggcaccc tgccctattc gggtcagcgc tttgcgctcg atccgggaac
     6541 ccggcagcaa gccgacaaaa ccaacgtctg cgtcgtacaa gtgagcgacg gcacgtattc
     6601 ggttacgcac ctagcgctgc gcggcggtga gctacagccg tttggaattc cgtagctgct
     6661 actgcgccga ttgaaccgtc atttcgcctt cgtcatcgct cgactgcgac ttggtaggtg
     6721 aaagaccctt gcaattggcg gtgtcgggcg caaaacgcgg cgcaaacttg tagacggaaa
     6781 ggaccagctg caagttgaaa tcaccgacgc tggcatccgc ttcgcgatcg gtcgaaaagc
     6841 gcatcccttg tactttggtc gatccgcagg gatcgaactt gccgtcgctc tgtttggtca
     6901 gccgctcaaa ctcatctttt gccaggtctt tggaacaggg ctgtgagccg tcggtgcagc
     6961 gaaagccgaa cgtgcgatac cagcgcttcc atttttcgcc atattcgctg atgcgcagcc
     7021 tgggttcggc gccctgcgtc ggattggcag cggtgacgtg gtagcgaacg cggtacgcgc
     7081 cgggttttag atcgacgtag atacagcgcg cttcggggct tgggccgatc ttgacgaacg
     7141 tgccgacgcc ctcgatggtg atgcgcatgg tgtcgtcgcc gcgtccggtg cgtagctcaa
     7201 agcgcttgaa gcccggagca ggaggactcg actcggcatc ggtttccgct gcgccacggc
     7261 ccgccgcatc gatctcgcga cacgtacaga agccgttttg acagcgcggc gacgatcccg
     7321 aaagcgagcc gatcagctcc tgaacatccg aagtatccag cacctgcggc ggcagcttgc
     7381 ccgagccacc accgctcgac gtttcggcac gcggacccgc acacgcggac gcagcaagca
     7441 agcaggctac ggccaagtct cgataaacgc gcaaagaatc ccccatggcg tcatcgtacc
     7501 acgacgcccc gcctggctga ttcggacgat gcagcggcta tcgagcgaac ttgcctttga
     7561 agatcagctc ttggacggga cgacgtgcgc tctggacttc gcgctcgcgc agccgctctg
     7621 gcagctcttc caccgggccg ggatgcccga tcgccatcat cacattcacc gcgaactgct
     7681 ccggcacctc gagtgcgacg cgcgctcgct cggcatcaaa gccgcccatc tgcaagaaga
     7741 cccgacgccg tacggctcca
//
LOCUS       DAORGN010000221         7745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4313_length_7745_cov_6.480624, whole genome shotgun sequence.
ACCESSION   DAORGN010000221 DAORGN010000000
VERSION     DAORGN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_7745_cov_6.480624"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKL17_18435"
     CDS             complement(<1..611)
                     /locus_tag="PKL17_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46768.1"
                     /translation="MSDRIDPRRQTREIREALGRYEPEQLLDILTHVFRQYVVDGSAP
                     PPMQLQDELSGLSFAQVIERLQLRLDLPELRLFEIQAGRVMVRVDGRLYPLETAESRP
                     EPLPVQRVVSMQPMAPAAPPAPAAAPPAPAQGPAPAAENRDAAPGVHSVGHASAARPQ
                     PAAQPARPSPTAAPAARPTAPAARPAATPSQAPATQAAAPAAPA"
     gene            complement(657..1346)
                     /locus_tag="PKL17_18440"
     CDS             complement(657..1346)
                     /locus_tag="PKL17_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNK46769.1"
                     /translation="MADVVESKEQVLIRYRDKELRGCTIKKLWLQGISLIGADLTDVT
                     FEDCSLDEANLTGAQLLRTKFNRTSARSANMTSATVTRATFTDCNLTELCLEKALVEG
                     VEFARCDMDNLRGDRAYFGNCSLFPNRAYGIKFRGATLIKLKVSVENGAPELIRAQLS
                     DATVIESELPQANLLRADLRRALLVKCNLHRALLRDAVVDQCTLVQCVTMGAELPTAL
                     VGGSALSQPLA"
     gene            complement(1368..1988)
                     /locus_tag="PKL17_18445"
     CDS             complement(1368..1988)
                     /locus_tag="PKL17_18445"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNK46770.1"
                     /translation="MTKQAGAAPQEKDEQAFRLDQEIAKKYDPGMLSRLIMRDAGRGE
                     PLDLHTRSAMERRLGGNFGGVRVYRGPLAEEVTARYRADAVTVGGTEMILVREGWQSN
                     FSTVQGGALLAHELTHVRQQQRGLHFHGAGDTSSPLEREAHSEQDSYARDFGHQADNA
                     SHEEYAKDLKAHMTEVMKEALKLYEKEKQQQDVRSNPNSQSEYGSD"
     gene            2159..3460
                     /gene="bioA"
                     /locus_tag="PKL17_18450"
     CDS             2159..3460
                     /gene="bioA"
                     /locus_tag="PKL17_18450"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNK46771.1"
                     /translation="MADSWATRDLRAIWHPFTQQAEWQPLVIVAGEGNFLIDEHGKRY
                     LDGVSSLWANVHGHRQPQIDAAIAAQLGNIAHSTLLGLSHPPAIELAERLVAIAPPGL
                     SRVFYSDSGSTAVEVALKMAFQFHQHGGDKRRQRFLALRNAYHGDTLGSVSVGGIDLF
                     HQLFSPLLFHTERVQPTISALEQAFAQHGSELAAFVVEPLIQGAAGMLLQPPGFLARA
                     RELCDRAGVLLIADEVATGFGRTGKMFACEHVGVSPDFLCLAKGLTGGYLPLAATLTT
                     EAVYDRFLGKRSEYKTFFHGHTFTGNPLGCAAALANLERFQSHRIVEQLPAKIANLQK
                     ALDVQLASHPNVQEIRRCGLMVGVEIGQDRHTAFPADLAMGVRVCEAVRRHGVILRPL
                     GDVVVLLPPLSITDDEIELLVGATRRALDEVVAGLAQDGSR"
     gene            3457..4155
                     /gene="bioD"
                     /locus_tag="PKL17_18455"
     CDS             3457..4155
                     /gene="bioD"
                     /locus_tag="PKL17_18455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNK46772.1"
                     /translation="MKRFLLVTGTDTGVGKTTVSVALLAAWRRRGHLVRPQKPIETGL
                     DDRSTESDAFRLAQAVGIDGHATSWLRFRLPVAPEAAARAEGQVIDPDRLIAHCRQLP
                     GDHILIEGAGGLLVPIAKGFVVADLAQSLGARLLVVARTRLGTINHTLLTLHEAQRRG
                     LCVAGLVLNRTVDEIGPEETDNVRLLREHADGGTPDPLGPLPFSESQAPDVLAALAEA
                     HLPVGDLFLRAFAD"
     gene            4300..5046
                     /locus_tag="PKL17_18460"
     CDS             4300..5046
                     /locus_tag="PKL17_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNK46773.1"
                     /translation="MRTAPSETAETASEGGAHDARQRICLAAFQLFGTRGYSCTSIQD
                     IADAAAVKKSILYYYFASKEDLYQSLLVESATHLRTLLHQGLQAADLAVETVSQRASA
                     EAGAEATLATLAETVLSLARENRESVRFFLSHIFAVDADRPPCSAVSVEQVPHTLILA
                     AIESGVASGELAGDPRLLECLVLGGIQVSIIRHLRSPEQQPLPTGYGRLLVRAALEGF
                     RPAVGKLVRATQKQSISDGASRPHKKVSRA"
     gene            5039..6238
                     /locus_tag="PKL17_18465"
     CDS             5039..6238
                     /locus_tag="PKL17_18465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK46774.1"
                     /translation="MRRATLYLFVSLFAVAGCKKDAAKQPNPATAQQKTVRVETAKAV
                     KHTFSGRLPITGELKPVQEVVLKSKVGGTVVVLNFDEGDLVKKGDLIAKIDSGNQQAQ
                     LRSNAAAVAIAEAQLQRSRADFERVSRDLERVEKLAASGAADQKTLDDTKSAMKLANV
                     SVSAANAQLDQARAVRDLAKNAVGETKYIAPITGVISRRGVSLHEYVDTMKNREIVTI
                     VDNTAMELVAQLAADLASGITKGARVEFQVNGPDAKPIEGEVTAVSPTVDPRTRSIRL
                     RVRIPNVDGKLKGGMYATGNVIAGGERQGIGVPVTSVREESISPPADLDGDGEDDKQS
                     VLWRIVGESVEKVVVRTGVTDGDLTEVVGGIQPDDQVVISSPAGLKPGVKVVVQHSTA
                     VNQHKNP"
     gene            6296..>7745
                     /locus_tag="PKL17_18470"
     CDS             6296..>7745
                     /locus_tag="PKL17_18470"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNK46775.1"
                     /translation="MVLSDISIKRPVFITMIMLAFVVLGGFAFKRLSVDEFPNVDLPV
                     VTVTTAWPGAGPESVESDISKKIEEALNTVAGVKQISSQSLEGVSSVVVVFHLHVKIN
                     DAANDVREKISRIKADLPQDAKDSLIERLDPGDRPILALALSSESMSLKELTDLSDTS
                     LRKKLENVPGVGKVTLVGGTKREIEVLLDPARLDARNLLPTDIINGLKSSNVDLPAGR
                     IERGSQETLLRVAGRVKEVRDFSELPIRMPTGGVTRLADVATIRDGTEEKRSLSLVAK
                     GQWQEGSKEFNVKQSVALEIRKQSGQNTVQVADAVREKMAELRSDLPQDVLLDVVIDN
                     SIFIRASVEQVEEDLLLGAILTVAIVFLFLQSWRSTVITGLTLPISVIGAFTAIWAAG
                     FTLNQLTLLALTLAIGLLIDDAIVVRENIVRHAEMGKDHVRAAREGTDEIGLAVLATT
                     FSIIAVFVPVAFMGGIIGRFFFQFGITISVAVM"
BASE COUNT         1434 a         2220 c         2492 g         1599 t
ORIGIN      
        1 gccggagcgg ctggggcggc agcttgggtc gctggggcct gactcggggt cgctgcgggt
       61 cgtgccgccg gggccgtcgg tcgtgctgcc ggggctgcgg tggggcttgg gcgggccggt
      121 tgcgcggctg gttggggtcg cgctgcgctg gcatggccga cggaatgaac gccgggtgcc
      181 gcgtcacggt tttcagcggc gggtgctggg ccttgggccg gagcaggcgg ggcggcagcc
      241 ggagcaggcg gggcggcggg cgccatcggc tgcatgctga cgacccgctg gaccggcagc
      301 ggctctgggc ggctttcggc ggtttccagc ggatagagtc tgccgtcgac gcggaccatg
      361 acccggcctg cttgaatttc aaacagtcgc agctcgggca gatcgagccg aagctgaagc
      421 cgctcaatca cttgcgcaaa actgagcccg ctgagctcgt cttggagctg catcggcggt
      481 ggcgcgcttc cgtcgacgac gtactgacgg aaaacgtggg tcaggatgtc gagcagttgc
      541 tccggctcat agcggcctag tgcctcgcgg atttcacggg tctggcgtcg ggggtcaatg
      601 cgatcgctca tggttatcct tgcatgacaa agcgggaaga catcgtcgcc acgcggttac
      661 gccagcggct gactgagcgc cgatccaccg acgagagccg tcggcagttc agcgcccatc
      721 gtgacgcact gcaccagggt gcactggtcg accacagcgt cgcgcaagag cgcgcgatgg
      781 aggttgcatt tgacgagcag tgctcgtcgc agatccgcgc gcaaaaggtt ggcttggggc
      841 agctcgcttt cgatgacagt cgcgtcggaa agctgggcgc gaatgagctc gggcgcaccg
      901 ttttcgacgc tgactttcag cttgatgagc gtggcgccgc gaaacttgat gccgtaggcg
      961 cgattgggaa acagcgaaca gttgccgaaa tacgcccgat cgccgcgcag attgtccatg
     1021 tcacagcggg caaattcgac gccttcgacg agcgcttttt caaggcacag ctcggtgagg
     1081 ttgcagtcgg tgaacgtggc ccgcgtcacg gtcgccgacg tcatgttggc gctgcgggcg
     1141 cttgtgcgat tgaacttggt gcgcaaaagc tgggcgccgg tgaggttggc ctcgtcgagg
     1201 ctacagtctt caaacgtcac atcggtcagg tcggcaccga tgagcgagat gccctggagc
     1261 catagctttt tgatggtgca gccgcgtagt tctttgtcgc gatagcgaat cagcacctgc
     1321 tctttgctct cgaccacgtc cgccatgtcc cgcctctccg tcgctgttta gtcggaacca
     1381 tactcacttt gcgagtttgg attcgaccgc acgtcctgct gctgtttttc tttttcgtaa
     1441 agcttcagcg cttctttcat gacttccgtc atgtgcgctt tcaggtcttt ggcatattcc
     1501 tcgtgcgacg cattgtcggc ctgatgcccg aagtcccgcg cgtaggagtc ttgctcgctg
     1561 tgcgcttccc gttcgagcgg cgacgacgta tcgccggcac cgtgaaagtg cagaccgcgc
     1621 tgctgctgac ggacgtgcgt cagttcgtgc gccaaaagtg cgccaccttg caccgtcgaa
     1681 aagttgctct gccagccttc gcgcaccagg atcatttcag tgccaccgac ggtaactgcg
     1741 tcggcgcgat atcgggccgt cacttcctcg gcgagcggtc ctcgatagac gcgaacgccg
     1801 ccgaaatttc cgccgagacg tcgttccatc gccgaccgcg tgtgcaaatc gagcggctcg
     1861 ccccgtcccg cgtcgcgcat gatgagccgc gacagcatgc cgggatcgta ttttttggcg
     1921 atttcctgat ccaagcgaaa tgcttgctca tctttttcct ggggagcagc gcctgcttgc
     1981 ttggtcataa agactcctcg cgtattagcc agtttacttc atttcattta tcgggtgagc
     2041 cgcgttcccg ttgtggtctg gccttgcccc ttgggtcgca atcgtggcat cagacttccc
     2101 caccatgacg gcatcttccc cacctgtgcg tcccacaccg aatcgaaacg aagctgtgtt
     2161 ggctgattct tgggctacgc gggatctgcg tgcgatctgg catcccttca cgcaacaggc
     2221 cgaatggcag ccgctcgtca ttgtcgctgg cgagggcaat tttctcattg atgagcacgg
     2281 aaagcgatat ctcgacgggg tttcgtcttt gtgggccaac gtgcacgggc atcggcaacc
     2341 gcagattgat gccgcgatcg cggctcagct tgggaacatc gcgcactcga cgctgcttgg
     2401 gctcagtcat ccgccggcga tcgaactggc cgagcgactt gtcgccatcg cgccgcccgg
     2461 actttcgcgg gtgttttatt ccgattcggg cagtaccgcc gtcgaagtcg cgctcaaaat
     2521 ggcgtttcag tttcaccaac atggcggcga caaacggcgg cagcgtttcc tggcacttcg
     2581 caatgcctat cacggtgaca cgctggggtc agtttccgtc ggtgggattg atttatttca
     2641 ccagcttttt tcgccgcttt tgtttcatac cgaacgggtc cagccgacga tttccgcgct
     2701 ggaacaggcg tttgcccagc acggcagcga gctggccgcc tttgtcgttg aaccgctgat
     2761 tcaaggcgcc gccggcatgc tgctgcagcc gccgggtttc cttgcgcggg cgcgcgagct
     2821 ttgcgatcgg gctggcgtgc tgctcatcgc cgatgaggtc gcgacgggat ttggtcgcac
     2881 cggcaagatg tttgcctgcg aacacgtcgg tgtttccccc gatttcttgt gtcttgccaa
     2941 gggtcttacc ggcggttatt tgccacttgc cgcgacgctg acgaccgaag cggtgtacga
     3001 ccgattcctt ggcaaacgca gcgaatacaa gacgtttttc cacggccaca cctttaccgg
     3061 caatccgctt ggctgtgcgg cggcgcttgc gaacttggaa cggttccaaa gccatcgcat
     3121 cgtcgaacag ctcccggcca agatcgcgaa tctgcaaaag gcgcttgatg tgcagcttgc
     3181 atcccatccc aacgtccaag agatccgtcg ctgtggtctg atggttgggg tcgaaatcgg
     3241 gcaagatcgg cacacggcgt ttccagccga tctggcgatg ggtgtgcggg tctgcgaggc
     3301 ggtgcgtcgc catggcgtca ttctgcgtcc gctcggtgat gtggtggtgc tgctgccgcc
     3361 gctatctatc accgacgatg aaatcgaact tctggtcggg gcaacacggc gtgcactcga
     3421 tgaagtggtg gcgggacttg cccaggatgg gtcgcgatga agcggttttt gctcgtgacc
     3481 ggaaccgaca ccggcgttgg caagacgacc gtcagtgtgg ccctgcttgc ggcgtggcga
     3541 agacgtggtc atttggtgcg tccgcagaag ccgatcgaga cgggactcga tgatcgatcg
     3601 accgaaagtg atgcctttcg gctcgcccag gcggtgggaa tcgacggtca cgcgacaagc
     3661 tggctgcgct ttcgtctgcc cgtggccccg gaagctgctg cgcgggccga aggccaggtc
     3721 atcgatcccg atcggctgat cgcccactgt cgacagctac ctggcgatca catcttgata
     3781 gaaggcgcgg gcgggctgct ggtgccgatc gccaaagggt ttgtcgtcgc agatttggcc
     3841 cagtcgctgg gggcgcggct tttggttgtg gcgcggacgc ggcttgggac gataaatcac
     3901 acgctgctga cgctacacga agcccagcga cggggtcttt gcgttgctgg tcttgtgctc
     3961 aatcgaacgg tcgacgaaat cggaccggaa gaaaccgaca acgtgcggct cttgcgcgaa
     4021 catgccgatg gcggaacgcc cgatccgctc ggaccgctgc cgttttccga aagccaagcg
     4081 ccggacgtgc tcgcggctct cgcggaagcg catcttcctg tcggtgacct gtttttgcgc
     4141 gcatttgcag actagggttt cagggcctgc gcttctggtt tttgcgatgg catccataag
     4201 cagggcgcat atctatcgac cgttcggtca aaaagtgctg gcggaaggtt gaccgatcgt
     4261 tcaaattatc cacaaatgac caagcggcgc gacctacaga tgcgtaccgc tccgtctgaa
     4321 acggctgaga ccgcttcgga gggcggcgct cacgatgcac gacagcggat ctgccttgca
     4381 gcttttcaac tgtttggcac ccgcggttat tcctgcacgt ccatccaaga cattgccgac
     4441 gctgcggcgg tgaaaaaatc gatcctctac tactactttg cgagcaaaga ggatctgtat
     4501 cagtcgctgc tcgtcgaaag cgcgacgcac ctgcgtacac tgctccatca ggggttgcag
     4561 gcggcggatc tggccgttga gacggtatcg caaagagcga gcgcggaggc tggggccgaa
     4621 gcaacgctgg cgacgctggc cgaaaccgtc ctgtcgctgg cgcgcgaaaa tcgggagtcg
     4681 gtccgtttct ttttgtcgca catctttgcc gtcgatgccg atcgtccgcc gtgtagtgcg
     4741 gtgtcggtcg aacaggtgcc gcacacgctg attttggcgg cgatcgagtc aggcgtggcg
     4801 tcgggggagc tggcgggtga tccgcggctt ttggaatgcc tcgtccttgg cggcattcag
     4861 gtttccatca ttcgtcatct gcgttcgcca gagcagcagc cattgccgac gggatacgga
     4921 cggcttttgg tgcgggcagc gctggaaggt tttcgtcccg ccgtcggaaa actggtgcgg
     4981 gcgacgcaaa agcagtcgat ttcggacgga gcaagccgtc cccacaagaa ggtgagccgt
     5041 gcgtagagca actctgtatc tttttgtttc attatttgcc gtcgctggtt gcaaaaaaga
     5101 tgcggccaag cagccaaatc cggcgacagc gcagcaaaag acagttcgcg tcgagactgc
     5161 caaagcggta aagcacacgt tcagcggtcg tttgccaatt accggcgagc tcaagccggt
     5221 gcaggaagtc gtgctcaagt cgaaagtggg cggcaccgtc gtggtgctca actttgacga
     5281 aggcgatctg gtcaaaaaag gcgatctgat tgcgaaaatc gattcgggaa atcagcaggc
     5341 gcagcttcga tcgaatgccg ccgcggtggc gattgccgaa gcccagctcc agcgcagccg
     5401 cgcagatttc gagcgggtga gtcgagacct ggagcgcgtc gagaaactgg ctgcgtccgg
     5461 ggctgccgat caaaagacgc tcgacgacac caagagcgcg atgaagctgg ccaacgtgtc
     5521 ggtgtcggca gcaaacgccc agcttgatca ggcgcgcgcg gttcgcgatt tggcgaaaaa
     5581 tgccgtcgga gaaaccaagt acatcgcgcc gattaccggc gtcatcagtc ggcgtggcgt
     5641 gtcgctgcat gaatatgtcg acacgatgaa aaaccgcgaa atcgtcacca tcgtcgacaa
     5701 caccgccatg gaactggtgg cgcagctcgc ggcggacttg gcgtcgggaa tcaccaaagg
     5761 cgcgcgggtc gaatttcagg taaacggccc ggacgccaag ccgattgagg gtgaagtgac
     5821 ggcggtcagt ccgacggtcg atccgcgaac gcgcagcatc aggctgcggg tgcgtatccc
     5881 gaacgtcgac ggcaagctca aaggcggtat gtatgcgacg gggaacgtga tcgccggcgg
     5941 cgaacggcag ggcattggcg tgcctgtgac gtcggtgcgc gaagaatcga tttcaccgcc
     6001 cgccgatctg gacggggatg gcgaagatga caagcagagc gtgctctggc gcatcgtcgg
     6061 tgaatcggtt gaaaaagtgg tggtgcgaac cggcgtgacc gacggcgatt tgaccgaagt
     6121 tgtgggcgga attcagcccg atgatcaagt ggtgatctcg tccccagccg ggctcaagcc
     6181 gggtgtcaag gtcgtggtcc agcactcgac ggcggtcaat cagcacaaaa atccgtaacg
     6241 cgctttgttt ccgggccgcc ggatgcgcgg ctcgtgaagt ggggtggagg acaccgtggt
     6301 tctctcagat atttcaatca aacggcccgt gttcatcacg atgatcatgc tggccttcgt
     6361 cgtgctcggc ggctttgcgt ttaagcgctt gtccgtcgat gagtttccca acgtcgactt
     6421 gcccgtcgtg acggtgacga cggcgtggcc gggcgcaggt ccggaatccg tcgaaagcga
     6481 catcagcaaa aagatcgaag aagcgctcaa caccgtcgcg ggcgtcaagc aaatcagctc
     6541 acagtcgctc gaaggcgtgt cgagcgtcgt cgtcgtgttt caccttcacg tcaagatcaa
     6601 cgacgccgcc aacgacgtgc gcgaaaaaat ctcgcgcatc aaagccgact tgccgcagga
     6661 cgccaaggac tcgctgatcg agcggcttga tcccggtgac aggccgattt tggccctggc
     6721 gctgtcgtcg gaatcgatgt cgctgaaaga gctgaccgat ctgtcggata cgtcgctgcg
     6781 caaaaaactg gaaaacgtac cgggtgtcgg caaagtcacg ctcgtcggtg ggaccaagcg
     6841 ggaaatcgaa gtgctgctgg acccggcgcg gctcgacgca cgcaacttgc tgccgacgga
     6901 catcatcaac ggcctgaagt catcgaacgt cgacttgccg gctggacgca ttgagcgagg
     6961 ctcgcaagaa acgctgctgc gcgtcgcggg tcgcgtcaag gaagtgcgtg atttttccga
     7021 gctaccgatt cggatgccaa cgggtggggt gactcggctg gccgatgtgg caacgattcg
     7081 cgacggaacc gaagaaaaac gcagcttgtc actcgtcgcc aaaggccagt ggcaggaagg
     7141 cagcaaagag ttcaacgtca agcagtcggt cgcgctggaa attcgcaagc agtctggaca
     7201 aaacaccgtc caagttgccg atgcagtgcg cgaaaaaatg gccgagctgc gcagcgatct
     7261 cccgcaggac gtgctgctcg acgtggtgat cgacaactcg atcttcattc gtgcgtcggt
     7321 ggaacaggtg gaagaagacc tgctgctcgg cgcaattttg accgtcgcga tcgtgtttct
     7381 gttccttcag agctggcgct cgacggtaat caccggactg acgctgccga tttcggtcat
     7441 cggcgcgttt acggcgatct gggcggcggg ctttacgctc aatcaactga cgctgttggc
     7501 gctgacgctg gcgatcggtc ttcttatcga cgacgcaatc gtggtgcgcg aaaacatcgt
     7561 ccgccacgcc gaaatgggca aagaccatgt gcgcgccgcg agagaaggca ccgacgaaat
     7621 cggtctggcg gtcttggcga cgacattttc gatcatcgcg gtgttcgttc ccgtcgcgtt
     7681 catgggcgga atcatcggtc ggttcttctt ccagttcggc atcacgatca gcgtcgccgt
     7741 gatgt
//
LOCUS       DAORGN010000222         7726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4328_length_7726_cov_6.476991, whole genome shotgun sequence.
ACCESSION   DAORGN010000222 DAORGN010000000
VERSION     DAORGN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_7726_cov_6.476991"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKL17_18475"
     CDS             <1..596
                     /locus_tag="PKL17_18475"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNK46776.1"
                     /translation="RDYAEWALDVPMFFVYRHGTYTPAGGMTFRRFMAEGFGGQQATL
                     ADWELHLSTLFPDARLKQYVELRTADAGPLDMVRALPSLWRGLFYSQSSLQAAWQLCA
                     DWSVEERAQLRADVPKRGFLAEVRGKSIAPLVEEMVQIAIAGLRELDSPGGIALLDPL
                     LCRAKERRCPADDILAVWNEVGGNPRAFVEKTRIRID"
     gene            596..2182
                     /locus_tag="PKL17_18480"
     CDS             596..2182
                     /locus_tag="PKL17_18480"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNK46777.1"
                     /translation="MAGRDTDRLVAISTWKKHARRTPEGYYDLETDDLGGVPVRLFLT
                     AKLLLEAEDTLYRQIVNAARFPGVKLVVITPDSHYGYGVPVGCVILTDAHAGAVAMGP
                     VGFDIGCGMMSARSSVEAEEATPEKRREFNRAVMERVEMGQGGRSTRMRNLEKREFDE
                     LIRGGAEYYVDKYGASFDRSRAERHRIPVEGEWDVPWGRQGRPERGIAQLGSLGGGNH
                     FIELQRSEQTGTLFVQVHTGSRGFGHGLATNYFELARAEKPDQVRDIDLGYFTPDSAH
                     YRSYLNAVAAGGNFAILNRLIIFEEIAAAFRQVFRRDLELVYEISHNLVQKEWHPEFG
                     DVLVHRKGATRAFPAGHPALDGTIWEKTGHPVLIPGSNKDYSFILRPKVQAAVSGFSV
                     NHGAGRQLSRSEAFRRLSQKSIDAEYRDAGILVNLDGRVPLDEAAPCYKSSREVVQAV
                     LDAGLAEVEHELWPLSSLKGTEDRSSAKKWKEKREKRAQRRQESDWQRYLVDAAVDDS
                     SDDDPDPSDNSGGDQSSHHY"
     gene            2294..3223
                     /locus_tag="PKL17_18485"
     CDS             2294..3223
                     /locus_tag="PKL17_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46778.1"
                     /translation="MSTVHHRLVVSCLSVALLCSSALPASARRHGKKTPDASEESKPA
                     DAKAEPSAAATPAPAAPAAAAPAAAAPPTASSPSAAATPAVAESPTNTAKEGAAREHF
                     MRGVDLYTQGSYASAWLEFTSAYQLVPKTALLNNMARCEVRIGRPVEALQHFKRYIAA
                     NPDDPDGTYIRQEIARLEGEVGRRNSPTEPEKSGVAEPASVPARRAPTASIIVGGLTV
                     GLLAAGAITLGMVSSRVSLLQSTCSPVCPNGDVSVIEQQSYAGYGLLGAAGVGAVATG
                     VLLYFELGKKRESNFKPIASWKSLSPYFVSRSD"
     gene            3224..4210
                     /locus_tag="PKL17_18490"
     CDS             3224..4210
                     /locus_tag="PKL17_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46779.1"
                     /translation="MKKLTLISLLSLVGCWDFQALSSGQTLSDAGPPPDFTMCPKTPN
                     SLQEDCTNPDADINNNCLPGCLDPTCATHSYCLASKSSYRGTGTPRPSSTSCVGETII
                     HQNLATQSCATACSCNTPSCTGAINVYDQTNCGGTATKITFPPAMACTTVNQTTPMMP
                     SVKLDPLTTTGCTPKAATVDIPPVWSGNVYLCENKGQNGLFADLVKSNDCLVFDGDTT
                     CPTDTFKNKTTYYTTTTGMVSCACACAPTGTSCAINAGQVRVADNNTCATGGANKNMN
                     VTQTNTCLPIVDATNASFNPKGVNVTAAAACQATGTVTATPNPSGQLTLCCK"
     gene            complement(4211..4789)
                     /locus_tag="PKL17_18495"
     CDS             complement(4211..4789)
                     /locus_tag="PKL17_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46780.1"
                     /translation="MSLSDSSAPSANEHAPVFRGQRFMVILIVVMLSVAGLAIWGLRS
                     SQHRRFAVSLSTIPPSANPAAVSVDKPLLLRVDHSSPRSRSLVSLAILLDADGNPLSE
                     IVPMPTVPGPKGVPTQQLEFPPLGKPPVRGLVSGLVMRVREDSPEARAKLSTALELTK
                     TKSHQLARVFTQVIVACRELDGQAEQAQREVK"
     gene            complement(4850..5701)
                     /locus_tag="PKL17_18500"
     CDS             complement(4850..5701)
                     /locus_tag="PKL17_18500"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNK46781.1"
                     /translation="MLLPRSLLVGLLVLLSMFSSGCSLRKVGYGLAAKYVSQRLIDTF
                     AIEGRDKIVAERAIRDIHKWHRQSELPRYVTLIDGLTDRLRDGMSDDDFRWLQQQGDE
                     AMARLADKFAPPAADVLSRLREDQLVAAERNLAKSEKERFDKLDQSDEKYFAYRLDNT
                     KKTLKTWLGSYTDEQLQIFAAFHREDRDEERKRREAMRANRAQLLQAVRSKQGKQELS
                     AMIVHWMTTRQTAPTPDYQQNEQRQEQRYARLLQQVDRTLSVKQRQHLIAELAAWKQD
                     FVALQAE"
     gene            5793..6500
                     /gene="rdgC"
                     /locus_tag="PKL17_18505"
     CDS             5793..6500
                     /gene="rdgC"
                     /locus_tag="PKL17_18505"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNK46782.1"
                     /translation="MGALSGGITLRRYRVQGNVPKDWKDVFEKGVRAHVLVPINVDGN
                     EDKSLGWCSIFDENDLDLSVEKFHFEGHVMLALRQDTLKPPAAMVKRLLKQRQQKIED
                     ERQQQLSAAALRDLKAILVAELRKTTPPKTRTVDVVWNLDEGRLAIYSHAKSMNEALL
                     ALFSQTFNLGLDLEGPALWAKEMTQDDGKQASQLKAARPTLELLHGFVGLRPTPHGHE
                     EGSQLASALRGELHQGA"
     gene            6503..7135
                     /locus_tag="PKL17_18510"
     CDS             6503..7135
                     /locus_tag="PKL17_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46783.1"
                     /translation="MAGGKRHGEPSLADQIAASGETEVNKATVALREDLTDQRFLGRE
                     FLTWLVYFCDDDGESGEFAGSDSVAAFRLRVGERAVLRALGDATGEIAARGPATGHSS
                     DVRYAIAGGLTVRELDLIFERDERLWMATVSAEHFDLKRVKLPELLSEEDSERVSERL
                     QLISDLDAMLKGLFALFLHDRLSSRWTKQTIPALRDWLKRSILEEKYLSA"
     gene            complement(7159..>7726)
                     /locus_tag="PKL17_18515"
     CDS             complement(7159..>7726)
                     /locus_tag="PKL17_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46784.1"
                     /translation="HKQAFQLILDNNVNCTAHAGEAYGPESIAQAIHYCGAHRIGHGT
                     RLRENGDLLNYVCDHRIPLEVCISSNIQTGAADSFETHPLPFYYTYGLRCTINTDNRL
                     VTNTTVSRELLLCHQHYGFTLEDLKDSIVAGFKSAFLPHREKSDLLKKVNAELDRYQD
                     PEDHGTIKHNSPKNNMLHHMRADISREP"
BASE COUNT         1530 a         2240 c         2456 g         1500 t
ORIGIN      
        1 tccgcgatta cgccgagtgg gcgctcgatg tgccgatgtt ttttgtctat cggcatggca
       61 cctatacgcc ggcgggtggc atgacgttcc gtcgcttcat ggcggaagga tttggcggac
      121 agcaagcgac gctggccgat tgggaactgc atctgtcgac gctgtttccc gatgcgcggc
      181 tcaagcaata tgtcgagctg cgtaccgcgg atgccgggcc tctcgacatg gtgcgggcac
      241 tgccgtcgct gtggcgcgga cttttttaca gtcagtcgtc gctgcaggcg gcgtggcagc
      301 tctgcgccga ctggtcggtc gaggagcgag cgcagctgcg cgccgatgtg ccaaagcgtg
      361 gctttttggc cgaggtgcgc ggcaaatcga tcgcgccgct cgtcgaggaa atggtgcaga
      421 tcgcgatcgc ggggctgcgt gagctggatt ctcccggcgg catcgccctg cttgatccac
      481 tgctttgtcg cgccaaagaa cgtcgctgtc ccgccgacga catcttggcg gtgtggaacg
      541 aagtcggcgg caatccacgc gcgttcgtcg aaaagacccg catccggatc gactgatggc
      601 tggccgcgac accgatcgtc tcgtcgcaat ttcgacctgg aagaagcacg ctcggcgcac
      661 acctgagggg tattacgacc tagagaccga cgatctaggc ggcgtgccgg tgcgactgtt
      721 tctgacggca aaacttcttc tcgaagcgga agacacgctg tatcggcaga tcgtcaacgc
      781 ggcgcggttt ccgggcgtca agctggtcgt gatcaccccc gactcgcact acgggtatgg
      841 cgtgccggtc ggctgcgtga tcctgaccga cgcccacgcg ggtgcggtcg cgatgggtcc
      901 ggtcggtttt gacatcggct gcggcatgat gagcgcccgt tcttccgtcg aagcggagga
      961 agcgacgccc gaaaagcgtc gcgagtttaa ccgtgccgtc atggagcgcg tcgagatggg
     1021 gcagggtgga cgcagcaccc ggatgcgcaa tctcgaaaaa cgcgagtttg acgagctgat
     1081 ccgcggcggc gccgagtatt acgtcgacaa atatggcgcc agtttcgacc gcagccgtgc
     1141 tgaaagacac cgcattcccg tcgaaggtga gtgggatgtc ccgtggggac ggcagggtcg
     1201 gcccgaacgc ggcatcgcac agcttggatc gctcggcggt ggcaaccact ttatcgaact
     1261 gcaaaggtcc gaacagacgg ggacgctgtt tgtccaagtc cacactggca gccgcggttt
     1321 tggccacgga ctggcgacga attactttga gctggcgcgc gccgaaaagc ctgaccaggt
     1381 tcgcgacatt gatttgggct actttacgcc cgattctgcg cactatcgaa gctacctaaa
     1441 tgccgtcgct gcggggggga attttgccat cctgaatcgg ctgatcatct tcgaggagat
     1501 cgccgccgca tttcgccagg tattccgtcg cgacctcgag cttgtgtacg aaatcagtca
     1561 caacttggtg caaaaagagt ggcatcccga gtttggcgac gtgctggttc atcgcaaagg
     1621 cgcgacgcgg gcctttccgg cgggacatcc ggcgctcgac ggcacgatct gggaaaaaac
     1681 cggccatccg gtgctgattc ccggcagcaa caaagactat tcgttcattt tgcgtccgaa
     1741 ggtccaggcg gcggtgtcgg gcttttcggt caaccacggg gcgggacggc aactttcgcg
     1801 cagcgaggcg tttcggcggc tgtcgcagaa gtcgatcgac gccgagtatc gcgatgctgg
     1861 aatcttggtc aatctcgacg ggcgcgtgcc gctcgatgag gcggcgccgt gctacaaatc
     1921 gtcgcgcgaa gtcgtgcagg cggtgctcga cgctggactg gcggaagtcg agcatgagct
     1981 gtggccgctg tcgtcgctca agggcaccga agatcggtcg agcgccaaaa agtggaaaga
     2041 aaagcgcgaa aagcgagcgc aaaggcggca ggaatccgac tggcagcgct atctggtcga
     2101 cgcggctgtg gacgattcga gcgatgacga tccagatccg agtgacaact cgggcggcga
     2161 ccaaagctca catcactact agcgccgtcg tgccaaaccg gttccgctcg gctcgctgcg
     2221 cagagtttgc gtaagtaccg atctgtactg actgtcgggt gggcctaagt gataaaatgg
     2281 acagacctgc atcatgtcta ctgtccatca tcgtctggtc gtaagctgtc tatcggttgc
     2341 gctgctctgt tcgtctgcat tgccggcaag tgcgcgacgg catggcaaaa aaacgcctga
     2401 tgcttcggaa gaaagcaaac cggcagacgc caaggcagaa ccgtccgcgg cggcgacgcc
     2461 tgcaccagcg gcaccagcag ctgcggcacc agcggctgcg gcaccaccga cggcgtcaag
     2521 tccgagcgcg gcagcgaccc cggctgtggc ggaatcaccg accaacaccg cgaaagaagg
     2581 cgcagcgcgc gaacacttca tgcgcggcgt cgatctgtat acccaaggca gctacgcaag
     2641 cgcctggctg gaatttacgt cggcgtacca gcttgtgccc aaaacggcgc tgcttaacaa
     2701 catggcgcgc tgcgaggtcc gcatcgggcg gcccgtcgaa gcgctccagc actttaagcg
     2761 ctacattgcg gccaatcctg acgatcccga tgggacgtac attcggcagg aaatcgctcg
     2821 actggaaggc gaggtcggtc gtcgcaacag cccgacagag cccgaaaaat cgggcgtcgc
     2881 tgagcctgct tctgtgcccg ctcgtcgggc gcccactgcc agcatcatcg tcggtggctt
     2941 gacggtgggt ctgctggctg ccggcgcgat cacgctcggg atggtcagca gtcgggtttc
     3001 cctgctccag tcgacgtgct cgccggtgtg cccaaatggc gacgtctcgg tcatcgagca
     3061 gcagtcctac gcgggctatg ggctgctcgg cgcggcgggg gttggcgcgg tggcgacggg
     3121 cgttctgctt tactttgagc ttggcaaaaa gcgcgaaagc aacttcaagc ccatcgccag
     3181 ctggaagtct ctgtctccgt actttgtttc gaggagtgac taaatgaaga aattaacgct
     3241 tatttcactg ctttctctcg tcggatgctg ggattttcag gcgctttcga gtgggcaaac
     3301 gctgagcgat gccgggccac cgcccgactt cacgatgtgt ccgaaaactc ccaattcgct
     3361 gcaagaggac tgcacaaatc ccgacgcgga catcaacaac aactgcctgc ctggctgtct
     3421 tgatccgacg tgtgcgacgc acagctattg cttggcaagc aaatcaagct atcgcggtac
     3481 gggtacgccg cggcccagtt cgacgagctg cgtcggtgaa acgatcattc accaaaacct
     3541 ggcgacgcag agctgtgcga cggcgtgttc gtgcaatacc ccaagctgca caggcgccat
     3601 caatgtgtat gaccagacga actgcggcgg cacggcgacc aagattacgt tccctccggc
     3661 gatggcctgt acgacagtaa accagaccac gccgatgatg ccgtcggtca agctggatcc
     3721 actgaccacg acgggctgta caccaaaagc cgcgacggtg gacattccgc cggtgtggtc
     3781 cggcaacgtc tacctgtgtg aaaacaaagg ccaaaacgga ctgtttgccg atctggtcaa
     3841 gagcaacgac tgcttggttt tcgacggcga cacgacgtgc ccgaccgaca cgtttaagaa
     3901 caagacgacc tactacacga cgacgactgg catggtcagc tgtgcatgcg cgtgtgctcc
     3961 gacggggact tcttgcgcga tcaacgccgg ccaggttcgc gtcgccgaca acaacacctg
     4021 tgcgacgggc ggcgccaaca agaacatgaa cgtcacccag accaacacct gcctgccgat
     4081 cgtcgacgca acaaacgctt cgtttaaccc caaaggggtg aatgtcaccg ccgccgcagc
     4141 atgccaagca acgggaaccg tgaccgcgac gccgaatccg tcgggtcagc tgacgctgtg
     4201 ctgcaaatag ctacttcact tcgcgctggg cttgctcggc ctgaccgtcg agttctcgac
     4261 aagcgacaat cacctgcgta aacacccgag cgagctggtg cgacttggtc ttcgtcagct
     4321 cgagcgcagt gctcagcttg gcgcgcgctt cggggctgtc ttcccgcaca cgcatcacca
     4381 gacccgaaac cagtccgcgc accggcggct tgccaagcgg cggaaactca agctgctgcg
     4441 tcgggacacc cttgggtcca ggcaccgtcg gcatcggcac aatttcgctc agcggattgc
     4501 catcggcgtc gagcaagatc gcaagtgaca ccaagctgcg gctgcgcggc gaggaatgat
     4561 caacccgcaa caaaagcggc ttgtcgacgg aaacagccgc tggattggcc gacggtggga
     4621 tcgtcgacag cgataccgca aagcgccgat gctggctgga acgcagcccc cagatcgcaa
     4681 gcccagcgac ggatagcatc accacaatca ggatgaccat gaagcgctgg ccgcgaaaca
     4741 ccggggcatg ctcgttcgca cttggcgcgg aagagtcaga caaagacatg cgctttcctt
     4801 accacagccg tcgaaaaatc gctttttgcc gcgtacttat cgtcgctggc tattcggcct
     4861 ggagcgcaac aaagtcctgt ttccacgccg caagctctgc aatcagatgc tgccgttgtt
     4921 tgacagacag tgtgcgatcg acttgctgaa gcagccgtgc gtagcgctgt tcctgacgct
     4981 gctcgttttg ctgatagtcc ggcgtcggtg ccgtctgccg cgtcgtcatc cagtgaacaa
     5041 tcatcgccga aagttcctgc ttaccctgct tgctgcgcac cgcttgcagg agctgggcgc
     5101 gatttgcccg catcgcctcg cgtcgcttgc gttcctcgtc gcgatcttcg cgatggaacg
     5161 ccgcaaaaat ttgcagctgt tcgtctgtgt agctgccgag ccaggtcttc agcgtctttt
     5221 tggtattgtc cagacggtag gcgaaatact tttcgtcgga ctgatcgagc ttgtcgaagc
     5281 gctctttttc gcttttggcc aagttgcgtt ccgccgcgac gagctggtct tcgcgcagac
     5341 gggacagcac gtcggcagcg ggcggcgcaa acttgtctgc cagacgagcc attgcctcgt
     5401 cgccttgctg ctgaagccag cggaagtcgt cgtcgctcat cccgtcgcgc agccgatcgg
     5461 tcagtccatc tatcagcgtg acgtagcgcg gcagctcgct ttgacggtgc cacttgtgga
     5521 tgtcgcggat cgcgcgctca gcgacgattt tgtcgcgtcc ctcgatggca aacgtgtcga
     5581 taagtcgctg cgagacgtac ttggccgcca gcccgtagcc gactttgcgc agactgcatc
     5641 ccgacgaaaa catcgacaaa agtacgagca gaccgaccaa cagcgagcgc ggtagcagca
     5701 tgtcttgcat gatagacccg cacgccgaga ctcgcccgtc cggtagggaa aaaagtttgc
     5761 gcagaaaacc tccctaagta ccatgcgccg ccatgggagc tctgtcgggt gggattacgc
     5821 tgcgtcgtta ccgagtgcaa ggtaacgtgc caaaggactg gaaagatgtc ttcgaaaagg
     5881 gcgttcgtgc acacgttctg gtgccgataa acgtcgacgg caacgaagac aaaagcctgg
     5941 gctggtgcag catctttgat gaaaacgatc tcgatctgtc cgtcgagaag tttcattttg
     6001 aaggccatgt gatgctggcg cttcggcaag acacgctcaa gccaccggcg gcgatggtga
     6061 agcggctgct caagcagcgt cagcaaaaaa tcgaggacga acgccagcag cagctgtcgg
     6121 cggcggcgct tcgcgatctg aaagcgattc tcgtcgcgga gctgcgcaaa acgacgccac
     6181 ccaagacccg caccgtcgat gtggtgtgga atctagatga aggtcggctg gcgatttatt
     6241 cgcacgccaa gtcgatgaac gaagcgctgc tggcgctgtt ttcgcagacc ttcaacctgg
     6301 gactggacct ggagggtccg gcgctgtggg cgaaggaaat gacccaggac gatgggaagc
     6361 aggcttcgca gctgaaagcg gcgcgtccga cgctggagct attgcacggc tttgttggat
     6421 tgcgtccaac accgcatggc catgaagaag gttcgcagct ggccagtgcg ctgcgcggcg
     6481 aactacacca aggagcgtga cgatggcggg cggaaaacgg cacggagaac cgagcctggc
     6541 ggatcaaatc gctgcctcgg gggaaaccga agtaaacaaa gccacggtcg cgctgcgcga
     6601 agacctgacc gatcagcgct ttttgggccg cgagtttttg acttggctgg tctatttctg
     6661 cgatgacgac ggcgaaagcg gtgagtttgc cggcagcgat tcggtcgctg cgtttcgtct
     6721 gcgcgtcggc gaacgagccg tcttgcgagc gctcggcgat gcgaccgggg aaattgccgc
     6781 gcgcggtcca gcgacgggac actcatcgga tgtgcgctat gcgattgccg gtgggctgac
     6841 ggtgcgtgag ctggatctga tctttgaacg cgacgagagg ctgtggatgg cgacggtgtc
     6901 ggcggaacat ttcgatctca agcgggtcaa gctgccggag cttttgtcgg aagaagacag
     6961 cgagcgcgtc agcgagcgac ttcagctcat ttccgatctc gacgcgatgc tcaaagggct
     7021 gtttgcgctg tttttgcacg accgtctgag cagccgctgg accaagcaga cgatcccagc
     7081 gctgcgcgac tggctgaagc gttcgatcct cgaagaaaaa tacctgagcg cctaagcggt
     7141 cgccgtcgcg cggatcggtt acggttcgcg tgagatgtcg gcgcgcatgt ggtgcagcat
     7201 gttgttcttc gggctgttgt gcttgatcgt cccgtggtct tcgggatctt ggtagcgatc
     7261 cagctcggcg ttgaccttct tcagaaggtc gcttttttcg cggtggggca aaaatgcgct
     7321 cttgaagcca gcgacgatgg agtccttgag gtcctcgagc gtaaagccgt agtgctggtg
     7381 gcagagcaaa agctcgcggc tcaccgtcgt gttggtgacg agccggttgt cggtgttgat
     7441 cgtgcagcgc agcccgtagg tgtaataaaa cggcagcggg tgcgtctcga agctgtcagc
     7501 ggcgccggtc tggatgtttg agctgatgca gacctcgagc ggaatgcggt gatcgcagac
     7561 gtagttgagc agatcgccgt tttcgcgcag gcgggtgccg tggccgatgc ggtgggcgcc
     7621 gcagtagtgg atggcctgag cgatcgactc ggggccgtag gcctcgccgg cgtgggcggt
     7681 gcagttgacg ttgttgtcga ggatgagctg gaacgcctgc ttgtgc
//
LOCUS       DAORGN010000223         7704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4342_length_7704_cov_5.475748, whole genome shotgun sequence.
ACCESSION   DAORGN010000223 DAORGN010000000
VERSION     DAORGN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4342_length_7704_cov_5.475748"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKL17_18520"
     CDS             <1..405
                     /locus_tag="PKL17_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46785.1"
                     /translation="VSEMVMKVDQLLKSAYQHAAAELGKIAEAAALGIHVYGVKLKDG
                     KHAIPIIDYLWLRSDNQFAEFDPTVTISYEEVVAKYDVNIVKTRIKESASKYADFQKN
                     FMEKLKKEPNTIKEIKNKRTPKPKKPSHLHSV"
     gene            445..879
                     /locus_tag="PKL17_18525"
     CDS             445..879
                     /locus_tag="PKL17_18525"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46786.1"
                     /translation="MPVLLIDGKSLWESRVIFQYLQKTHGKPGLSLDEENIVGAIDTL
                     QDQLIQPFLLQRFGHVVDRNNPYYQRVADRQRLILAYLEKQAESGLLSRWDYPAMSLF
                     SMLDWASFRNVLPDSELGPALVRFTKECATKPLVSETDPRKA"
     gene            complement(888..1487)
                     /locus_tag="PKL17_18530"
     CDS             complement(888..1487)
                     /locus_tag="PKL17_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNK46787.1"
                     /translation="MNFKSAVLAIGLLALPALGHAANYEIDPVHSSAQFAVKHLMVST
                     VRGEFTKLSGTAVIDDKNLGKSTIEASIDAATISTREAKRDEHLRSPDFFDTAKFPSI
                     TFKSTEVKKAGDGKYKVTGNLTLHGVSKSVVLDVDAPATEVKDPYGNVKRGATATTSI
                     NRKDFGLGWNKALETGGVLVGESVQITIDLQLVRKDAGK"
     gene            1580..2461
                     /locus_tag="PKL17_18535"
     CDS             1580..2461
                     /locus_tag="PKL17_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNK46788.1"
                     /translation="MDLNLLALFVEVAKTASFTAAGARLGLPRSSVSRSIAQLEVDLG
                     VPLFNRTTRKVSLSTAGAALFERVGPQLQELRKALGSLPEREEEPSGVLLITAPTDIG
                     VAFLPDAIAAFRLRYPAVTIDARLTPRRVDLVAEGFDLALRVSVKPLSDSSLLVRKLS
                     ELEMALYAAPSYLLRRGNIRSPEDTQDHEWVYFPTAPPLPPFPKLIKRSGVSGDDLLF
                     VHSAIRAGMGIGFLPSFLAREDVAAGKLVQLLPRHTVRTGTLYFVYPKGHTVPRKVTA
                     FRNFLLEYLTTRPLSLR"
     gene            2577..3482
                     /gene="cysD"
                     /locus_tag="PKL17_18540"
     CDS             2577..3482
                     /gene="cysD"
                     /locus_tag="PKL17_18540"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNK46789.1"
                     /translation="MTMNLTHLQRLEAESIHILREVVSECEKPVMLYSIGKDSAVMLH
                     LAKKAFFPSKPPFPLLHVDTTWKFRDMISFRDRMAKEDGWQLITYVNQDGLAKGINPF
                     ASGSGLHTDVMKTQALRQALDLYGFDAAIGGARRDEEKSRAKERVFSFRSAEHRWDPK
                     SQRPELWRLYNARKHPKESIRVFPLSNWTELDVWQYIYLEQIPIVPLYYAAPRPVVER
                     GGLLIMVDDDRMPLLSGEVPMIKKVRFRTLGCYPLTGAIESEADTLPAIIQEMLLTTT
                     SERQGRAIDHDQAASMEKKKQEGYF"
     gene            3482..5365
                     /gene="cysN"
                     /locus_tag="PKL17_18545"
     CDS             3482..5365
                     /gene="cysN"
                     /locus_tag="PKL17_18545"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNK46790.1"
                     /translation="MSHKSSLIAESIEAYLNAHQHKGLLRFMTCGSVDDGKSTLIGRL
                     LYESKLIFSDQLAALEAESRKRNVPPGELDLSLLVDGLAAEREQGITIDVAYRFFSTD
                     RRKFIVADSPGHEQYTRNMVTAASNVELAILLVDARKGVLTQTRRHSYLVSLLGIQKV
                     VLAVNKMDAVDFSEEVFLAIAKDYLAFAQQIGLSDVLAIPLSALKGDNVTSLSERTPY
                     FRGPTLMEYLETVTVEENLSHKPLRMPVQWVNRPHQDFRGFSGLIASGTVRPGDRVRV
                     MPKGVVTEVKGITTYDGDLPSAVAGQSVTLTLRDEVDVSRGDILVDAASPPGVTDQFQ
                     ATVVWMHETELLPGRPYLIKLGTSFATATVSALKYKVNVNTLEKLAARTLSQSEIGVC
                     NLSLDRALAFDSYADNRDTGGFILIDRMTNNTVGAGLIHFALRRADNIHWQAIDLNKE
                     ARALQKGQKPCVLWFTGLSGSGKSTIANLVDKQLYAQGRHSYILDGDNVRHGLCKDLG
                     FSPSDRVENIRRVAEVAKLMVDSGLIVLVAFISPFRAERQLARSLFAEGEFLEIFVDT
                     PLAEAERRDPKGLYKKARAQQLKNFTGIDSPYEAPESPEIRIDTTTVSAEDAAAQIVY
                     RLG"
     gene            5495..7291
                     /locus_tag="PKL17_18550"
     CDS             5495..7291
                     /locus_tag="PKL17_18550"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46791.1"
                     /translation="MSQAAPHTDLTPVADDRLGKKVGPYVLHRRIGQGGMGVVYEAEH
                     EHIGQRAAVKILMRDLARDARVLQRFLDEAKAMTMVQHEGIVKVFDYGQLPDGVPYIL
                     MEYLEGQSLQARLSQATNEGSGLPVSSALEIARQVASAIAALHQKKIVHRDLKPDNLI
                     LVRDSLMMGGERVKLLDFGLAKVMNQQVRRTTDGSILGTPLYMSPEQWEGGRRLGPKS
                     DVYALGAMLFELLSGRPPFVEDTPAQLMFRHVNTEAPLLSDLATTVPPRLDGLLALML
                     AKGPATRPDMTEVQSLLTPLCLELAGRGPLACRQGVVVPASMGMSDGMGETLGAEPRA
                     LQADALRAAASALPSTTPPHEGSAFASSEALEPLISMGLRDPLSSPVPRDSRPLPALP
                     KSADVPPAPSVANARSSPSSPALPQLTSPSLPQIASAEQGQPSGRLRSVSRDALIVEM
                     PPPAARSTSLSGLSVSLPPAPQNRQLRWLFVGAGLIVLLLLSVGALALRSGRAKNQAA
                     QRPSQVVVMPSPNAPVAPVVQPVPALSVTAQPAAVANPPSVVPVAPSDPAVPSAEGGK
                     APTSKGKRRSVSKKQRVDATSVDDPMTVFREH"
     gene            7307..>7704
                     /locus_tag="PKL17_18555"
     CDS             7307..>7704
                     /locus_tag="PKL17_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46792.1"
                     /translation="MRAFLVALALLFVPMLGRAAEEQPEPCPAGWEPGSPLYSQVLIV
                     PPLVALPELEPMPQGWPPKESVGELAEHAIRMFAEKNFRDSIERLEVAYAIDPQPLFL
                     FNMAQAFRLSDDPRQAIVYYQRFQQLAPTHR"
BASE COUNT         1577 a         2111 c         2350 g         1666 t
ORIGIN      
        1 gtttctgaaa tggtaatgaa agtggatcaa ttgcttaaat cggcatatca acatgctgcc
       61 gccgaattag gaaaaattgc agaagcggcg gcacttggta tccacgttta cggagtaaaa
      121 ctaaaagatg gaaaacacgc cattcctata attgactact tgtggttaag atcggataat
      181 caattcgcgg agtttgatcc gactgttaca atttcatacg aggaagtcgt tgctaagtac
      241 gacgtaaaca ttgtaaaaac aagaataaaa gaatccgcta gtaagtacgc ggattttcaa
      301 aaaaacttta tggaaaagtt aaaaaaggaa ccgaatacaa ttaaagaaat aaagaacaaa
      361 cgtactccaa agccaaaaaa acctagccat cttcattccg tttgaacgtc tttggtcgga
      421 ccgtcaaatt agaccactat ccgaattccg gtgctgctga tcgatggaaa gtcgctgtgg
      481 gaatcgcggg tcattttcca gtatctgcaa aagacccatg gcaagccggg gctctcgctc
      541 gacgaagaaa acatcgtggg ggcgattgat acgctgcaag atcagctgat ccagccgttt
      601 ttgcttcagc gctttggtca tgtggtggac agaaacaatc cgtactatca gcgcgtcgcc
      661 gatcggcagc gactgatcct tgcgtatctg gaaaaacaag cagagtctgg acttctgtct
      721 cgctgggatt atccggcgat gagcctgttt tcgatgctgg actgggcttc cttccggaac
      781 gtcctacccg acagtgaact cggccccgca ctcgtccgtt tcacaaaaga gtgcgcgacc
      841 aaacccctcg tttccgaaac cgacccccgc aaggcctgac ccgcgcgcta cttgccggcg
      901 tctttgcgga caagctgaag gtcgatggtg atctgcacgc tttcaccgac caggacgccg
      961 cctgtttcga gtgctttgtt ccagcccaga ccaaagtctt tgcgattgat ggacgtcgtc
     1021 gctgtcgcgc cgcgcttcac attcccatag gggtctttga cttcggttgc cggcgcatcg
     1081 acatccagca cgaccgactt gctgacgccg tgcagggtca ggttgcccgt cactttgtac
     1141 ttcccatcgc cggccttttt cacttccgtg gacttgaagg tgatcgatgg aaacttcgcc
     1201 gtatcaaaga aatccgggct gcgcaggtgt tcgtcgcgct tggcttctcg cgtgctgatc
     1261 gtggctgcat caatggacgc ttcgatggtc gacttgccca ggtttttgtc gtcgatgact
     1321 gcggttcccg acagcttggt aaactcgccg cgcacagtcg acaccatcag gtgtttcacc
     1381 gcgaactggg cgctggagtg aaccggatcg atttcgtagt ttgccgcgtg tccaagggcc
     1441 ggcagcgcca acagtccgat tgccaaaaca gcagacttga agttcatgat cgtgctctcc
     1501 tttgacagcc agtctgtgat tggtctgaaa ttcgcactag atatgttttt tgcgtcaaac
     1561 tgttgctacc atcgcaacaa tggatttgaa ccttctggcg ctgtttgtcg aggtcgccaa
     1621 gaccgcttcc tttaccgctg cgggggcacg acttggactc ccgcgatcgt cggtgagccg
     1681 cagcattgca cagcttgaag tcgacttggg cgtgccgctt tttaaccgca ccacgcgcaa
     1741 ggtttcgctg agcactgccg gggcggcgct gtttgaaagg gtggggccgc agctgcaaga
     1801 gctgcgcaaa gcactgggca gtctgcccga acgggaagag gaaccgtcgg gtgtgctgct
     1861 catcaccgca ccaaccgaca tcggcgtggc gtttctgcct gacgccattg cggcgtttcg
     1921 gctgcgttat cctgcggtga caattgatgc gcggctgact ccgcggcggg tggacttggt
     1981 ggcggaaggg tttgatctgg cgctgcgtgt cagcgtcaag ccactgtcgg attcgtcgct
     2041 gcttgtgcgc aagctgagcg aactggaaat ggcgctgtat gcggcgcctt cctatctttt
     2101 gcggcgcgga aacattcgca gtccggaaga cacccaggat catgaatggg tttactttcc
     2161 gacggcgccg ccgctgccgc cgtttcccaa actgatcaaa aggagtggtg tgtcaggcga
     2221 cgatctgctg tttgttcaca gcgcgattcg ggctggcatg ggaatcggtt ttctgccgag
     2281 ctttctggcg cgggaagatg tcgcagcagg caagctggtg cagcttttgc cgcggcatac
     2341 cgtgcgcacc ggaacgctgt attttgtcta tccgaaaggg cacacggtgc cgcgcaaagt
     2401 cactgcgttt cgcaacttcc tgctcgaata cctgacgacg cgaccgcttt ctctgcgctg
     2461 atagaaggtc gcgctcgttt ggctggatgg acctttgttt gggggtccgc aggccgccgc
     2521 agcgattcgc tgatgacatt ttcctgtcgt cggtgtacat cgctcaaaag caggacatga
     2581 cgatgaattt gacgcaccta cagcggctgg aggctgagag cattcacatt ctgcgggaag
     2641 tggtttccga atgcgaaaag ccagtgatgc tctattcgat tggcaaagac agcgcagtga
     2701 tgctgcacct ggcgaagaag gcgttcttcc caagcaagcc gccgtttccg cttctgcacg
     2761 tcgacacgac gtggaagttt cgcgacatga tctcgtttcg cgatcggatg gccaaagaag
     2821 atggctggca gcttatcacc tatgtgaatc aggacggact tgcgaaagga atcaatccgt
     2881 ttgcaagtgg ttcgggactc cataccgatg tgatgaagac gcaggcgctg cgacaggcgc
     2941 tggatctgta tggttttgat gcggcgattg ggggtgcacg ccgcgacgaa gaaaagtcac
     3001 gcgccaagga gcgggtgttt tcctttcgca gcgccgaaca tcgctgggac cccaagtcac
     3061 agaggccaga gctgtggcga ctttacaacg ccagaaagca tcccaaagag agcatccggg
     3121 tctttccgct gtcgaactgg accgagctgg atgtctggca atacatctat ctggaacaga
     3181 ttccgattgt gccgctgtat tatgctgcgc cgcgtccagt cgtcgagcgt ggcggactcc
     3241 ttatcatggt cgatgatgat cggatgccgc tcctttcggg ggaagttccg atgatcaaga
     3301 aagtgcggtt tcggacgctg ggttgttatc cgctgacggg ggcgatcgaa agcgaagcgg
     3361 acacgctgcc tgcgatcatt caggaaatgc tgctgacgac cacgtcggag cggcagggca
     3421 gggcgattga tcacgatcaa gcggcttcga tggaaaagaa gaagcaggag ggatacttct
     3481 aatgtcacac aagtcttccc tgattgcaga gagcatcgaa gcgtatctga atgcgcatca
     3541 gcacaagggt ctgcttcgct ttatgacgtg cggcagcgtc gacgatggca aaagcacgct
     3601 gatcgggcgg cttctgtatg aatcgaagct gatcttttcg gatcaactgg cagcactgga
     3661 agcggagtcg cgcaaacgga atgtgccgcc gggtgagctg gacttgtcgc ttttggtcga
     3721 cgggcttgcg gcagagcgcg agcaaggaat caccatcgac gttgcgtacc gtttcttttc
     3781 gactgatcgg cgcaagttca tcgttgcaga ttctcctggg cacgagcagt acacgcgcaa
     3841 catggtgacg gcggcgtcga atgtcgagct ggcgatcctg cttgtcgatg cgcgcaaagg
     3901 agtcctgacc cagacgcggc ggcacagtta tctggtgtct ctgttgggga ttcagaaagt
     3961 cgtgctggcc gtcaacaaga tggatgcggt cgacttttcc gaagaagtct ttttggcgat
     4021 cgcaaaagac tatctcgcgt ttgcgcagca gattggtttg tccgatgtgc tggcgattcc
     4081 gctctcggcg ctcaaagggg acaatgtaac ttcgctgagc gagcggactc cgtacttccg
     4141 gggaccgacg ctgatggagt atctggaaac cgtcacggtg gaagaaaatc tatcgcacaa
     4201 gccgctgcgc atgccggtgc agtgggtgaa tcgtccgcac caagacttcc ggggattttc
     4261 cggactgatt gccagcggca cggtaagacc aggtgacaga gtccgcgtga tgcccaaagg
     4321 agtcgtgacc gaagtcaaag gaatcacgac ctatgacggc gatctgccgt cggcagtggc
     4381 cggacaatcg gtgacactga cgctgcgcga tgaagtcgat gtgagccgcg gcgatatcct
     4441 ggtcgatgcg gcttctcctc ctggtgtgac cgatcagttc caagcgaccg tggtgtggat
     4501 gcatgaaacc gagctgttgc cggggcgtcc gtatcttatc aagcttggga caagctttgc
     4561 gacggcaacg gtatcggcgc tcaagtacaa agtgaatgtg aatacgctgg aaaaactggc
     4621 ggcgcggacg ctttcacaaa gtgaaattgg ggtctgcaat ctcagtctgg atcgggcgct
     4681 ggcgtttgat tcctatgcgg acaaccgtga cacgggtggt ttcattctga tcgatcggat
     4741 gaccaacaac accgtcgggg cggggctgat tcactttgcg ctccggcggg ccgacaacat
     4801 ccactggcaa gcgatcgatc tgaacaaaga agcgcgcgcg ttacaaaaag gccagaagcc
     4861 ctgcgtgctg tggtttaccg gactttcggg atcaggaaag tcaacgattg ccaacttggt
     4921 ggacaagcag ctctacgcac agggtcgtca ttcgtacatt ctcgatgggg acaacgtccg
     4981 ccacgggctg tgtaaagact tgggtttttc gccaagcgat cgggtcgaaa acatccggcg
     5041 ggtggcggag gttgcgaagc tgatggtcga ttccggtctg attgtgcttg tggcgtttat
     5101 ttcgccgttc cgggccgaac gtcagcttgc gcgcagtctg tttgccgaag gagagtttct
     5161 ggaaatcttc gtcgatacgc cgcttgccga agcggaacga cgcgatccca aagggcttta
     5221 caaaaaagcg cgcgcgcaac agctcaaaaa cttcacgggc attgactcgc catatgaagc
     5281 gccagaaagt ccggaaatac ggattgatac gacgactgtc tcggcggagg acgctgccgc
     5341 ccagattgtg taccgtttgg gatagtacct agcaaaatcg cgaacttttg gctccgatcc
     5401 gaatcggatc ctgtcgggcg acaaaccgca gcggtcgctc gtctgcatgg agcgaaaaca
     5461 gggtatgatg atggttcgga tagaaccatt tcggatgtcc caggctgccc cccataccga
     5521 tctgacaccc gttgccgacg atcgtcttgg caaaaaggtc ggtccctacg tgctgcatcg
     5581 ccggatcggc caaggcggca tgggcgtcgt ctacgaagcc gagcatgagc acattggtca
     5641 gcgcgcggcg gtgaagatcc tgatgcgcga cttggcgcgc gatgcccgcg tacttcagcg
     5701 gtttctcgac gaagcgaagg cgatgacgat ggtgcagcac gaaggcatcg tcaaggtctt
     5761 cgactacggc cagcttcccg acggggtgcc ctacatcctg atggagtatc tggaagggca
     5821 gtcgctgcaa gcgcggctga gtcaggcgac gaacgaaggg tcgggtctgc cggtcagcag
     5881 cgcactagaa attgcccgac aagtcgccag tgcgatcgct gccctccacc aaaagaaaat
     5941 cgtccatcgc gacctcaagc cggacaacct gatcctggtg cgggactcgc tgatgatggg
     6001 cggggaacgg gtgaagctgc tcgactttgg cttggccaaa gtgatgaacc agcaagtccg
     6061 tcgaaccacc gacggtagca tcctggggac gccgctttat atgtcgcccg agcagtggga
     6121 aggcggcagg cggcttgggc ccaagtcgga tgtgtatgcg ctgggggcga tgctgtttga
     6181 gctgctctcg gggcggcctc cgtttgtcga ggacacgccg gcgcagctga tgttccgtca
     6241 cgtcaacacc gaggcgccgc tgctgtcgga tttggcaacg acagtgccgc cacggctcga
     6301 cgggctgttg gcgctgatgc tggcgaaggg accggcgacg cgacccgata tgacggaagt
     6361 gcagtcgctg ttgacgccgc tgtgtctgga actggccgga cgcggaccgc ttgcctgtcg
     6421 acaaggtgtg gttgttccgg cgagcatggg gatgagcgac gggatgggcg agacgctggg
     6481 cgccgagccg cgcgcacttc aggcagacgc gctgcgtgct gccgcatccg cgctgccctc
     6541 gacgacacct ccgcacgagg gatcggcgtt tgcgtcttcg gaagcacttg agccgctcat
     6601 ctcgatgggg ctgcgcgatc ctttgtcatc gcctgtcccg cgggactctc ggcctttgcc
     6661 ggcgctgccc aaatctgcgg acgtgcctcc tgcgccgtcc gttgcgaacg cgcgatcgtc
     6721 tccgtcttca ccagcgctgc cgcagctgac gtcgccgtct ttgccgcaaa ttgcatcggc
     6781 agaacagggt cagccatcgg gccggctgcg gtcggtgtcg cgtgacgcgc tgattgtgga
     6841 aatgccaccg ccggcggcgc gcagtacctc gctgtcgggg ctttcggtgt cactgccccc
     6901 ggcgccgcag aatcgtcagc ttcgctggtt gttcgttggt gcaggtctga ttgtgctgct
     6961 tctgctttcg gtcggtgcgc tggcgctgcg cagcgggcga gccaaaaacc aagcggccca
     7021 gcgaccgtcg caagtcgtgg tgatgccgtc tccgaatgcc cctgtggccc cagtggtgca
     7081 gcctgtgccc gcgttgagcg tgacggcaca gcctgccgcc gtggccaacc ctccgtccgt
     7141 tgttcccgtc gcaccgagcg atcccgcagt cccatcggca gagggtggca aagcgccgac
     7201 ttccaaaggc aaacgccgct cggtcagcaa gaagcagcgc gtggatgcga cgagcgtcga
     7261 tgaccccatg accgtgttcc gcgagcacta atccaaggtg atcgttgtgc gggcattcct
     7321 tgttgcgctg gctttgctgt tcgtcccgat gctgggcagg gccgccgaag aacaaccaga
     7381 gccctgtccc gcgggttggg agcccggctc gccgctgtat tcgcaagttt tgatcgtgcc
     7441 accgcttgtg gccctgccgg agctggagcc catgccgcag ggctggcctc cgaaagaatc
     7501 cgtcggggaa ctggccgagc acgcgatccg gatgtttgcg gaaaagaact tccgcgattc
     7561 gatcgaacgg ctcgaagtcg cctatgcaat cgatccgcag ccgctgtttt tgttcaacat
     7621 ggcgcaggcg tttcggctct ctgacgatcc acggcaagcc atcgtctact atcagcggtt
     7681 tcagcagctc gctccaaccc atcg
//
LOCUS       DAORGN010000224         7703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4346_length_7703_cov_5.283604, whole genome shotgun sequence.
ACCESSION   DAORGN010000224 DAORGN010000000
VERSION     DAORGN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_7703_cov_5.283604"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKL17_18560"
     CDS             complement(<1..296)
                     /locus_tag="PKL17_18560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK46793.1"
                     /translation="MLLYRWLNEAVENRGTAKALVYRDTYLSWRGMQHRVERRAQEFA
                     AMGIEKGHWVGLMLGNVPDFAILALALSRLGATVVPLDPTTGVRDLTLFLEHAPL"
     gene            complement(443..838)
                     /locus_tag="PKL17_18565"
     CDS             complement(443..838)
                     /locus_tag="PKL17_18565"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNK46794.1"
                     /translation="MTIALWCLLAAVILPYVWLPVSFGERQKQFGNVDNKQPRSQVAK
                     LEGKGARAYAAHQNAFEALAVFAPAVLTAHVAKADPTLSGALAAVWVVCRFLHGVMYV
                     ADIDKARSGMFFLAFLSAIGQFVLAAMAH"
     gene            complement(920..3724)
                     /gene="mutS"
                     /locus_tag="PKL17_18570"
     CDS             complement(920..3724)
                     /gene="mutS"
                     /locus_tag="PKL17_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNK46795.1"
                     /translation="MSTSLALDSDSDQAEHALPAALEKAKPASSRSAIPSVDTPVMRQ
                     YLEIKERYPDCILFFRLGDFYEMFFDDAVEASQLLDLTLTARDKHKEVPVPMCGIPYH
                     AVRGYVGRLIAQGKKVAICDQVEDAKKAKKIVRRAVTQVITPGVVLEEDQLEPKSSNY
                     LCAICPGGAARAGLAYLDVSTGEFAACELPEAEALDELCRIEPAELLFVDDKTQPGPS
                     RGAEWLAKIAQRLKIPTGHAQRTDPAADAALLSEMQQSGTVSTENLTETGLLSAAAAA
                     CLRYARATQPSGGLPIIELRRYRPSDCMVLDDATKTNLELLQTLLERKRQGSLLGVID
                     QTKTAMGGRLLRSWLLAPLLDLDAIGSRHDAVEWLVDRQAARQALREVLRGIHDLERL
                     TGRLTTLLATPRDLFSLGASLRELPRLGDILRSGLTQKTDQILPELPLLLSLGTDLCE
                     DIAATIGNAICDNPPVQWRDGGFLRRGYHAELDEYADLSQGGKDHIQRIEDREKERTG
                     ISSLKVRYNKVFGYFIEITRTNLGKVPSDYVRKQTTVNGERFVTDELAEYEAKVLHAE
                     ERRVELELTLYESLRQSLATQATRLLKLAHRVAQLDVLCGLAELAHRSSYCRPTMTRD
                     LRLQIVEGRHPVIEQLRERGTFVPNDTTLDPDGEQILVLTGPNMAGKSTVMRQVALIC
                     ILAQMGSFVPAQRAELGLTDRVLTRVGATDNLSRGDSTFMVEMRETSNILQRATKRSL
                     VVLDEIGRGTSTYDGLSIAWAVAEYLHDVSGCKTLFATHYHELTALAAARPRVRNAQV
                     AIRQYQDEIVFLHKLLPGGANRSYGIEVARLAGLPRPLLSRAREILAALETQATSQGT
                     SSLPSHGVPPLQSGQLSLLLGAMAQPDPPTSPPAQAPALSPKMREVIDRIKMTDVDDL
                     SPRAAHDLLRHLGQLVRASQ"
     gene            3814..4848
                     /locus_tag="PKL17_18575"
     CDS             3814..4848
                     /locus_tag="PKL17_18575"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46796.1"
                     /translation="MNTLRTLLPYALLSLLSLGSSCSFSLPERMICKDDGCTSGNVDM
                     AQPVDMVNPRTDPNADGPFLVASQDVTVPASLNLSQNKVTMVWPSDDGMNPSTKNATY
                     PLVVMLPAQSLSPSQMRSYSDRLATHGIAVALVKASDERRQVHYRDSVVALIPYLVQM
                     SDVKGKLSADRVGVFGYQLGGMIAVSSVVRSAGALRAAFVVDPVAVLSFVEPLDGLSE
                     ASLVNMGKDTPLFVIGEPTSKTSDPGILPCTPADANYEQFYSRVAGSATAVTFPGATL
                     GDFVNTYPDNCGTPTMDRATTLRLTQKFATAYFQWTLLDRSSASDHLLGLGFAADRTL
                     YGLSQVKKGL"
     gene            4971..5267
                     /locus_tag="PKL17_18580"
     CDS             4971..5267
                     /locus_tag="PKL17_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46797.1"
                     /translation="MLRSVRVPAAFMPPFARAESFVEKLFSDVQRAPERGTIHIGGDR
                     YVLVRAESLYLSWFDALSDAFGEDAAREFIYKRYSLRKKQAISSSSDGVGIGLQ"
     gene            5349..6437
                     /locus_tag="PKL17_18585"
     CDS             5349..6437
                     /locus_tag="PKL17_18585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNK46798.1"
                     /translation="MIRKTKDGKFIGWYLRFIDVDGKRKQRASKQPTQAAARRMLVEI
                     EARIVRGKLGVPEREEPLRLTMAQLTERFLSEYDNPRIRDLTRWRQKQRYVFVSILKD
                     IGELPISTFSVDAAERFRNRLVRTYKPNTARNKLASLTLLLSWATRKGFLLSNPLQGL
                     RQPTATMRLQYLTKEEAIRLLDVAEQRAKNSLHDASIAVAIQLGIYGGLRVGEICGLR
                     FSDINLGAGIITVKRSYDRTTTKSGKERTIPIAPELAESLRSWKGICPETPERLVCPC
                     LILGRWRMPMRAPSLARFYEAARLPIPSAPWHILRHSFASLFMMNGGSILTLQRLLGH
                     ADIKQTQIYAHLSQDFVATEIRRLTLRS"
     gene            6625..6930
                     /locus_tag="PKL17_18590"
     CDS             6625..6930
                     /locus_tag="PKL17_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46799.1"
                     /translation="MNLGIWFAGPSVLVSSQVMRPLDTSPAADERYHELLRRMPPEKR
                     LEAAMRLSVGVRELALAGIRQRFPFASEQELRVRLAVRLYGRASVARAFRDIPPDAT"
     gene            6935..7522
                     /locus_tag="PKL17_18595"
     CDS             6935..7522
                     /locus_tag="PKL17_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46800.1"
                     /translation="MNSDEPINDSLDLALRVGNVLESLGCDYFVGGSVASSLHGEPRA
                     TNDIDFVVAMRPQRVQAFAEKLGDDFEVDTDMLRDALVHGSCANFFYLPLVTKIDFFA
                     LGSARYDEMEFQRRQRVRVRSTGETLFVKSPEDTVLRKLLWYRAGGEESSKQWRDVVE
                     VLRISGPVLDLSYMERWAVELKINDLLRRAHSQSV"
BASE COUNT         1556 a         2239 c         2342 g         1566 t
ORIGIN      
        1 agcggagcat gttccaaaaa cagcgtaaga tcgcgcacac ctgtcgtcgg atcgagggga
       61 acgaccgtcg ccccaagccg cgacagcgcc agcgccagga tcgcaaaatc gggcacattg
      121 ccgagcatca gcccgaccca gtggcctttt tcgattccca ttgcagcgaa ctcttgggcg
      181 cggcgctcga cacggtgctg catgccccgc cacgagagat aggtatcccg ataaaccagc
      241 gccttggcag tgcctcggtt ttcaacagcc tcgttgagcc agcggtacag cagcatgaca
      301 agcacccgaa atcacaaggg ttagagcttt gcgaaacgga ctttagcctg cttgtcatcc
      361 gccatacaag gacatacagc gtccgacgat ggcggcgagg tgggagcgga ggatgaccgg
      421 gcggacggag atcgaaaagc gcttagtgcg ccatcgcggc gagcacgaac tgaccgatgg
      481 ccgacaggaa cgccaggaag aacatgccag agcgggcttt gtcgatgtca gcgacgtaca
      541 tcacaccgtg gagaaagcgg cagacgaccc acaccgcggc gagcgcaccc gacagcgtcg
      601 gatcggcttt ggcgacatgc gcagtcagca ccgccggagc gaacacggcg agcgcctcaa
      661 acgcattttg gtgggcggcg taggctcggg cgcctttgcc ttccagcttg gcgacttgcg
      721 agcgcggctg cttgttgtcg acgttgccga actgcttctg gcgttctccg aatgaaacgg
      781 gcagccagac gtagggaagg atcaccgcag caagcagaca ccaaagagcg atcgtcatga
      841 gcaaacctcc aagagaaagg actttgccca ctttatcagg agccaagctt ttttcggggc
      901 caagcagcgg ggcggacggc tactggctcg cgcggacgag ttggcccagg tggcgcaaca
      961 ggtcatgcgc ggcgcgtggc gacaggtcat ccacgtcggt cattttgatt cgatcgatca
     1021 cttcgcgcat tttcggggac agcgcggggg cttgtgctgg tggactcgtc ggtggatctg
     1081 gctgggccat cgcaccaagc aaaagcgaca gctgacccga ttgcagaggc ggcacaccgt
     1141 gcgatgggag cgacgaagtc ccttggcttg tggcttgggt ttcgagcgcc gccaggattt
     1201 cacgcgcccg actgagcagc ggccttggca gaccggcgag ccgcgccact tcgatgccgt
     1261 aggatcgatt ggcaccgccg ggcaacagct tgtgcaaaaa cacgatctcg tcttgatact
     1321 gacggatcgc gacttgggca ttgcgcaccc gtggacgcgc cgccgccagc gccgtcagct
     1381 cgtggtagtg cgtcgcaaac agcgtcttgc agcccgacac atcgtgcaga tattcagcga
     1441 cggcccacgc gatcgacagc ccgtcatatg tcgatgttcc acgcccaatt tcatccagca
     1501 cgaccaggga ccgcttcgtc gcccgctgca agatgttgct ggtttcgcgc atttcgacca
     1561 tgaacgtcga atcgccacgc gacaggttgt ccgtcgctcc aacccgtgtc agcacccgat
     1621 cggtcagtcc cagctccgcc cgctgcgccg gcacaaacga tcccatctgc gccagaatgc
     1681 agatcagcgc tacttgccgc atcaccgtcg atttgcccgc catgtttggc ccggtcagca
     1741 ccaggatttg ttcgccatcg ggatcgagcg tggtgtcgtt gggcacgaag gtgccgcgct
     1801 cgcgaagctg ttcgataact gggtgacggc cttcgacaat ttgcagccgc agatcgcgcg
     1861 tcatcgtcgg acgacagtag cttgaacggt gcgccagctc ggccagacca cacagcacat
     1921 ccagctgcgc cacccgatgg gccagcttga gcagccgtgt cgcctgggtc gccaggctct
     1981 gacggagcga ttcatacagc gtcagctcta gctcgacacg gcgttcctcg gcgtgcagca
     2041 ctttcgcttc gtattcggcc agctcgtcgg tgacaaagcg ctcgccgttg acggtggtct
     2101 gtttgcggac gtagtcgctc gggactttgc ccaggtttgt gcgtgtgatc tcgatgaagt
     2161 agccaaagac tttgttatag cgaaccttca gcgacgaaat cccggtgcgt tctttttcgc
     2221 ggtcctcgat gcgctggatg tgatctttgc cgccttgcga cagatcggcg tattcatcca
     2281 gttccgcatg gtagccgcgt cggagaaagc cgccatctcg ccactgtacc ggcgggttgt
     2341 cgcagatggc gttgccgatc gtcgccgcga tgtcttcaca cagatcggtc ccaagcgaaa
     2401 gcagcaacgg cagctcgggc agaatttgat cggtcttttg ggtcaaacca ctgcgcaaaa
     2461 tgtcgccaag ccgtggcagc tcgcgcaggc ttgccccaag agaaaacaga tcgcgcggcg
     2521 tcgcgaggag cgtcgtcagt cgtccggtca accgctccag gtcgtgaatg ccgcgcagca
     2581 cctcgcgcag cgcttgtctg gccgcctgac gatcgaccaa ccactcgacg gcatcatgcc
     2641 gtgatccgat tgcatccagg tcgagcagcg gcgccagcag ccagctgcgc aaaaggcgtc
     2701 cgcccatcgc ggtcttcgtc tgatcaatca caccgagcag cgatccctgg cgtttgcgct
     2761 ccagtagcgt ttggagtaac tccaaattgg tcttggtcgc atcgtcgaga accatgcagt
     2821 cgctgggccg atagcggcgc agttcgatga tcggcagacc gcccgacggc tgggtcgccc
     2881 gtgcatagcg cagacacgcc gccgcagccg ccgaaagaag cccggtttcc gtcaggtttt
     2941 ccgtcgatac cgtgccggat tgctgcattt ccgaaagcag agccgcgtcc gccgccggat
     3001 cggttcgctg cgcatgtccc gtcggaattt tgagacgctg cgcaattttg gcaagccatt
     3061 cggctccgcg cgacggtccg ggctgggttt tgtcatcgac aaaaagcagc tcggcgggct
     3121 caatgcggca cagctcgtcg agcgcttcgg cttcgggcag ctcacaggcg gcaaattctc
     3181 ccgtcgacac gtcgaggtac gcgagcccag cccgcgcggc gccgcccgga caaatcgcgc
     3241 acaaataatt cgatgacttg ggttcgagct gatcttcttc gaggacaacg ccaggcgtga
     3301 tgacttgcgt gacggcgcgg cggacgattt tttttgcttt tttggcgtct tcaacctgat
     3361 cgcagatcgc gactttttta ccctgcgcaa tcagcctgcc gacgtagccg cgcaccgcgt
     3421 gatacggaat gccgcacatc ggcaccggca cttctttgtg cttgtcgcgt gccgtcagcg
     3481 tcaagtcgag cagctgcgac gcttcgacag catcgtcgaa gaacatttcg taaaaatcgc
     3541 cgagccgaaa aaacaaaatg cagtcgggat agcgctcttt gatttccagg tattggcgca
     3601 tcaccggcgt atcgacggaa ggaatcgcac tgcgtgacga cgcgggtttg gctttttcca
     3661 gtgccgcggg caaagcgtgc tcagcctgat cgctgtcgga gtcgagtgcc aatgatgtgg
     3721 acatggcagc cgtttagcag accacgaagc ggaaggtaaa gcgcgtggta cgatttggtc
     3781 gagagactca gtcctcagcc ccggtgcact cccatgaata cactgcgaac cttgcttccc
     3841 tatgcgctgc tcagcttgct ttcgctcggg tcttcttgct cgttttccct accggaacgg
     3901 atgatctgca aagacgacgg ctgcacgagc ggaaacgtgg acatggcgca gcctgtcgac
     3961 atggtgaatc cgcgcaccga cccaaatgca gacggaccgt ttctggtcgc gagccaagat
     4021 gtgacggtgc ccgcgtcgct gaatctttcg caaaacaaag tcacgatggt ttggccgagc
     4081 gacgatggaa tgaatccatc gaccaaaaac gcgacctatc cgcttgtcgt gatgctcccg
     4141 gcgcagagtc tgtcgccaag ccagatgcgc agctattccg accgcttggc gacgcatggc
     4201 atcgcggtgg cgcttgtcaa agccagcgat gagcgacgac aagtgcacta tcgcgacagt
     4261 gtggtcgcgc tgattcctta ccttgtgcag atgtctgacg taaaaggcaa gctgtcggcg
     4321 gatcgtgtcg gggtgtttgg ctatcagctc ggtgggatga tcgctgtgtc gtcggtggtt
     4381 cggtctgccg gagcgctgcg cgcggcattc gtggtcgatc ccgtcgccgt gctcagtttc
     4441 gtcgagccgc tggatggact ttcggaagca tcgcttgtga acatgggcaa agacacgccg
     4501 ctgtttgtga tcggggaacc gaccagcaag acgagcgatc cgggaatttt gccgtgcaca
     4561 ccagcggacg caaactacga acagttttat tcccgcgtgg caggatcagc gacggcagtg
     4621 acgtttcctg gcgcgacgct gggtgacttt gtgaatacct atcccgacaa ctgtggaacg
     4681 cctacgatgg atcgggcgac gacgctgcgc ttgacccaga agtttgcgac ggcctatttc
     4741 cagtggacgc tgctcgatcg ctcgtcggca agtgatcatc tgctaggtct tggtttcgca
     4801 gccgatcgca cgctgtacgg actttcgcag gtcaaaaaag ggctgtaatc ccgcgctcga
     4861 ttccgtcccc caaaagatcc gctacaccga cagaaaatgg cgttcgattc tgtccccccg
     4921 aacgtgatac cgtaaaaagt gccaatcact actctttcgc ggagttcctg atgctacgga
     4981 gtgttcgcgt tcctgctgcg tttatgcccc cctttgcgcg ggcagaatcc tttgtcgaaa
     5041 agctgttttc cgacgtccag cgcgcccccg agcgcggcac cattcacatt ggtggcgatc
     5101 gctacgtcct ggtccgggca gagagtctgt acctaagctg gtttgacgcc ctgagcgacg
     5161 ccttcggcga agacgccgcc cgtgagttta tctacaagcg atatagctta cgtaaaaaac
     5221 aagcaatatc cagcagttcc gacggtgtgg gcattggctt acagtagcga actcgaccgc
     5281 actgacgagg ccaatcaccg catgcgggga ccacgggagg cccaaaacgg ggcaagcgaa
     5341 tgggcgaggt gattcggaag acaaaagacg gcaagttcat cggctggtat ctgcgcttca
     5401 tcgatgttga tggcaagcgc aagcagcggg cctcgaaaca gccgacgcaa gcggcggcgc
     5461 ggcgcatgct ggtcgagatt gaagcgcgca tcgttcgtgg caagctcggg gtgcccgagc
     5521 gagaagagcc gctgcggctc acgatggcgc agctgaccga gcgattccta tctgaatatg
     5581 acaacccacg gatacgggat ttgacacgat ggagacagaa gcagcgctac gtgttcgtct
     5641 cgatcctgaa ggacattggc gagctgccga tttcgacatt ttccgtcgat gcggcggagc
     5701 ggtttcggaa tcgcctggtc cgtacctaca agcccaatac ggcgcgcaac aagctcgcga
     5761 gcctaacgct gctgttgtca tgggcaaccc ggaagggatt ccttctcagc aatccactgc
     5821 aaggtcttcg tcagccgaca gcaacgatgc gtctgcaata cctaaccaaa gaggaagcga
     5881 tacgcctgct tgatgttgcc gaacagagag caaagaacag tctgcacgat gcctcgattg
     5941 cggtggcgat tcagctaggt atctatggtg gcctgcgggt cggggagatt tgcggactcc
     6001 gcttttcgga catcaacttg ggcgcaggca tcatcaccgt taagcgaagc tacgaccgga
     6061 ccaccaccaa gagcggcaaa gagagaacga ttccgatagc ccccgagctt gcagaatcgc
     6121 tccgtagctg gaagggaatc tgtcctgaaa ctccagagag actcgtctgt ccgtgtctga
     6181 ttctgggaag gtggcggatg ccgatgcgag caccaagcct tgcgcgcttt tatgaagccg
     6241 cgcggctgcc gataccgtca gcaccatggc acattctgag gcactccttt gcctctctgt
     6301 tcatgatgaa cggaggaagc atcctgaccc tgcagcgcct tctcggccac gccgacatca
     6361 aacagaccca gatttatgcg cacctaagcc aagacttcgt tgccacagaa atccgccgcc
     6421 tgacactgcg aagctgagtt tcccaatctc agccgcgtag tcagtagaat gacgccgctc
     6481 ggctgtgcgg gagcctctgc aaacagccaa tccatcgccg ataaatacaa tcctacgctc
     6541 gacgtcttcg atgatccgga aacagaccta aacggatcct ctttgggtgc gcgagtttgg
     6601 tgggttcgcg agggacgcac ggagttgaat cttggaattt ggtttgctgg accgtcggtt
     6661 ctggtaagct cgcaggtgat gcgaccgctt gatacatcgc ctgccgctga cgagcgctac
     6721 cacgagttgc ttcggcggat gccgccggaa aagcggctgg aggcagcgat gcggctgagc
     6781 gtgggcgtgc gcgagctggc cttggcgggg attcggcaga gatttccttt tgcgagtgaa
     6841 caggagctgc gagttcggct ggcggtgcgt ctttatggtc gagcgagtgt ggcgcgtgcg
     6901 ttccgcgaca ttccgcccga tgcaacgtga tggaatgaac agcgacgaac cgatcaacga
     6961 ttcactcgat ctggcgctgc gcgtcgggaa tgtgctggaa tcgctgggtt gtgactactt
     7021 tgtgggcggt agcgttgcca gttcgttgca cggtgaacca cgagcgacga acgacatcga
     7081 ctttgtggtt gcgatgcgtc cgcagcgggt gcaggccttt gctgaaaagc tcggcgacga
     7141 ttttgaggtg gatacggaca tgctccgcga tgcgcttgtg catgggagct gcgcgaactt
     7201 tttctacctg ccgctggtga ccaagatcga cttttttgcg cttggatctg ctcgttacga
     7261 tgagatggaa tttcagcgcc ggcagcgtgt gcgggtccgt tcgacgggag agacgctgtt
     7321 tgtgaagtcg ccagaagata cggtactgcg gaagctcctt tggtatcggg ccggcggtga
     7381 agagtcctcg aagcagtggc gcgatgtcgt cgaggtgctc cgcatcagtg gtccggttct
     7441 cgacctttcg tacatggagc gctgggccgt cgaactgaag atcaatgatc tgctgcgacg
     7501 ggcgcattcg cagtcggttt aggaatacct gtttcgtcga caaacggcga ttccttcgcg
     7561 tggtccgttc gttcctccaa cgtgtggacc acaaaaggac cacgctttcc caatcgctgt
     7621 gctataccgt cgctgtcgag aaaccagggt gagtttatct acaactctgc gcgggaaatt
     7681 gggcggaatg actcgcggcg gtt
//
LOCUS       DAORGN010000225         7553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4466_length_7553_cov_6.338624, whole genome shotgun sequence.
ACCESSION   DAORGN010000225 DAORGN010000000
VERSION     DAORGN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4466_length_7553_cov_6.338624"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKL17_18600"
     CDS             <1..423
                     /locus_tag="PKL17_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46801.1"
                     /translation="HIRPGEARSKKGEARTVPIGGIDAEGWLRTGDRGKIDRTNRLTL
                     LGREDDLVKVEGKRVALGEVEGCLESYPKVKAAQARVFTDEDGSTMVVASVVLSGRCR
                     AEELIDHCAKNLAPYKVPRRVELRGSLTDELGGQECAR"
     gene            411..1616
                     /locus_tag="PKL17_18605"
     CDS             411..1616
                     /locus_tag="PKL17_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNK46802.1"
                     /translation="MRSLRNPLFLGLLALPLACNEARLDAEIDETGSEDMSDGYRRYD
                     LAGRDLARMRDRDAACAEVRAEATLEKKPVDIIFVIDNSGSMTEEILSVQNNINKNFA
                     DIIGKSGLDYRVIMVTSHGDARSRQSVCVSAPLSTSATCSPVPARPGNNPPRFYHYSI
                     EVESWDSFRRILGSVNGTEKDQFNLAPMGWQTWLRTDAYKVFIEITDDKSDMTETDFD
                     TQLLAKAPTQFGTKANRNYVWHTIAGLKENTPSTKPWGPMDPIQSGICTKGGGAVAAG
                     VEYQRLSMMTGGLRFPICEYANFDAVFNHVAMGVVSGAKVACDFALPSPPAGQQIDPG
                     SVLVEYTPGGMGSRSTFKQVAGPGSCGPNSFYIDKSRIYLCPDACNTVQADSKAKVEV
                     LFDCLSILG"
     gene            1688..2488
                     /locus_tag="PKL17_18610"
     CDS             1688..2488
                     /locus_tag="PKL17_18610"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNK46803.1"
                     /translation="MSSYLGPSFGHTGQVRKQRQQHEETLVKEALLGGLSNERREQIR
                     SQAQREIPAWYNPWVHLSATTGIGVLALCLGTWQLAKLGQIKPSDLLIIPITFVLANL
                     FEWRVHKHVLHRRRWPMEVLYDKHTPMHHMIYVEDDMTLRSQREFRLVLIPAVGVLGV
                     VLVTTPFALGIAALWSTQAGWLFLVTASLYMVTYELLHLAYHAPKDSFIGRRRFISAM
                     RRHHARHHNPRLMQRYNFNVIIPLSDYLLGTIAPASQRRDNPHKEEPS"
     gene            2485..3468
                     /locus_tag="PKL17_18615"
     CDS             2485..3468
                     /locus_tag="PKL17_18615"
                     /inference="COORDINATES: protein motif:HMM:NF012409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HNK46804.1"
                     /translation="MNLSTLHSAASYPHSVSKTTTAITAHFDKQRHPFRRFLFASLLL
                     CGISAGCSGTSSTGDNPSPSQDGATTNGSDGGTTGGAALSDLLPEALFNQIFLHRGTS
                     PCPGAFYTYQSFVDAARAFPGFATQGTTDDRKRELAAFLANISHETTGGWATAPDGPY
                     SWGLCFIHEGGNIDPSTLTSYCVASAQYPCAPGKKYYGRGPIQLSYNYNYGQAGAALG
                     VDLLNNPDLVGSDPVLAWKTALWFWMTPQAPKPSCHNVMIGQFAPSTQDLAAGRKPGF
                     GLTVNIINGGVECNQPTPAQVTDRVGFYNRYSQLLGVSPGTDLYCDKMQSY"
     gene            complement(3543..4583)
                     /locus_tag="PKL17_18620"
     CDS             complement(3543..4583)
                     /locus_tag="PKL17_18620"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HNK46805.1"
                     /translation="MRTQLLSVLSLLVSLGCSSADTDREQPNLGKPFHYPSDGELRLS
                     DVQVRGTHNSYHVDTHAGSVEAWAYTHAPLYDQLDRLGIRQIELDVFFDEQDGLQVQH
                     VPFLDTGTRCKQFSDCLSEIRRFSDAYPGHFPIYVQIEPKADVPESDMESFFAKLEGE
                     ILAAFPRERILTPDEVQADFPTLPAALKTRGWPLLQTLRGRVFFAFDTTGPLRTAYTH
                     KLRDLHGRLLFVDSSPTDSFAAVAVLNDPQDPAGVMNIGKAVRAGLLVRSRADADTKE
                     ARVSDTSRGQAALSSGAHFISTDYPEPVSGFSYHFEVPGGKPARCNPVTAPKSCTPLA
                     LEDPAFVGSANP"
     gene            4668..5153
                     /locus_tag="PKL17_18625"
     CDS             4668..5153
                     /locus_tag="PKL17_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK46806.1"
                     /translation="MSFQVVLRPVTESDLPTLFAHQDDPVVRAMGVIPHRAREEFFQK
                     WKEVLADTQFAKQSIWVDEQLVGNMVCFLRDGVHEIGYVVGRDHWGKGIASAALAQFL
                     PLISHLPRPITARVVKHNFASLRVLQKNGFLIVAEMTETDPQGRHIPEFRLSLAEEPN
                     Q"
     gene            5290..6309
                     /locus_tag="PKL17_18630"
     CDS             5290..6309
                     /locus_tag="PKL17_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46807.1"
                     /translation="MRTIRTFSLGSFSVSFLALALSAVWSCNAQIGDSPADASVDSAM
                     WTPPPPPDLRFDPDAFWANDPPLQVCVPDGGMLPPPPTPGGTPECPDDKNRQGCACPT
                     LGEKRSCWPGLRANRNLGICRDGETTCVRAGELGQAWGPCEGYVLPVPGATAGADACK
                     CFSAGRWALDNLVPCFYTSSGQDIGATSSVIQGGKPTCVSMSQPLQKPTSPWSPNTLT
                     VDCVGHWKLCYTLRAGDGKNPLPTDCVVATSCTEGDYTDANKPQALPQLPAWATTTAA
                     QIDCAKKFKATGGYGEMSVDGTTVTCDKVQKVFNRVNYCPLRCNDNPMLPECQNCGSG
                     GSGGF"
     gene            6436..>7553
                     /locus_tag="PKL17_18635"
     CDS             6436..>7553
                     /locus_tag="PKL17_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46808.1"
                     /translation="MATRTGAGKSAPKTPKATTAKKSTATKKATPAKAPKKAAAPKKT
                     AAPKKSAAPKKSAAPKKTAAPKKSAAPKAATSRRGAKVDLAAAAQTASEMAKSGAVVN
                     HIAFVVDRSGSMSGIRSKVIAAFNAQLAECRKNSESLAQQTFVSFYTFQTTVDPPRFF
                     AKPIADVSSLTELRCTGTTALLDATGQAIVDLSSMPSAELDNVSFLVLVLTDGAENNS
                     RKYKSTLKGMIEKVQATGRWTFAFLTPPGDEATLAGFGIPLGNIRAWETTQAGIKQLG
                     QAMQQGLQNFYQARSKGQKAIVGVFTTDLSTVSTTEVKTTLTEATGSFVTLPVAADCD
                     IRTLVEKSLGNGSFKKGNGYYELTKPELVQEYKEIAIV"
BASE COUNT         1678 a         2264 c         2185 g         1426 t
ORIGIN      
        1 catatcaggc ccggcgaagc ccgcagcaaa aaaggcgaag cgcgcactgt gccgattggt
       61 ggcatcgatg ccgaaggctg gctgcgaacg ggcgaccgcg gaaaaatcga tcgcaccaac
      121 cgactgacgc tgctcggacg cgaagacgat ctggtcaaag tcgaaggcaa gcgcgtcgca
      181 ctcggtgaag tcgaaggctg cctagagtcc tatcccaagg tcaaagccgc ccaggcccgc
      241 gtctttaccg acgaagacgg cagcaccatg gtcgtcgcca gcgtcgtgct cagcggtcgc
      301 tgtcgagccg aagagcttat cgatcactgc gccaaaaatc tcgctccata caaagtgccg
      361 cgccgagtcg agctgcgcgg ctcgctcacc gacgaacttg gaggtcaaga atgcgctcgc
      421 taaggaatcc cctcttcctc ggactgctcg ctctgccgct ggcatgcaat gaagcccgtc
      481 tggatgccga gatcgacgaa accggcagcg aagacatgtc cgacggctac aggcggtatg
      541 acttggcggg acgcgacctc gcgagaatgc gggaccgcga cgctgcgtgc gccgaagttc
      601 gggccgaagc gaccctggaa aaaaagccgg tcgacatcat ttttgtgatc gacaactcgg
      661 gcagcatgac cgaggaaatt ctgtcggttc aaaacaacat caacaaaaac ttcgccgaca
      721 tcatcggcaa aagcggtctc gattaccggg tcatcatggt cacaagccat ggcgatgcgc
      781 ggtcgcggca gtcggtctgt gtcagcgccc ccctatcgac gagcgcgacg tgcagccccg
      841 tcccagcaag gcccggcaac aacccgccgc gcttttatca ctacagcatc gaagtcgaaa
      901 gctgggattc cttccgtcgc atccttggtt cggtaaacgg cacggaaaaa gaccagttca
      961 acctcgcacc gatgggctgg caaacgtggc tgcgcaccga tgcgtacaaa gtctttatcg
     1021 agatcaccga cgacaaatcg gacatgaccg aaacggactt tgacacccag cttttggcga
     1081 aagcaccgac gcagtttggc accaaagcca accgcaacta tgtctggcac accatcgcgg
     1141 gtctgaagga aaacaccccg tcgacaaagc catggggacc gatggaccca atccaaagcg
     1201 gaatctgcac caagggtggt ggcgccgtcg ctgcgggcgt ggaataccag cggctttcga
     1261 tgatgacggg cggcctgcgt ttcccgatct gcgaatacgc caacttcgat gcggtgttta
     1321 accacgtcgc gatgggcgtc gtatcgggtg ccaaggtcgc atgtgacttt gcgctcccgt
     1381 cgccaccagc tggccagcag atcgatcccg gctcggttct ggtcgaatac acaccgggcg
     1441 gaatgggcag ccgcagcaca ttcaagcagg tcgctggccc cggtagctgc ggtcccaact
     1501 cgttctacat cgacaagagc cgcatctatc tgtgccccga cgcctgcaac accgtgcaag
     1561 ccgacagcaa agccaaagtc gaagttctgt tcgactgtct gtcgatcctc ggctgatcgt
     1621 ctccgcaccg atccccccgc ttcttccgcc cgcagctttt tccatccagc agtagactgg
     1681 gaagacgatg tcttcctatc ttgggccaag ctttggtcac actggacagg tacgcaagca
     1741 gcgccagcaa cacgaagaga cgctggtgaa ggaagcgctt ctggggggac tatcaaacga
     1801 acgtcgcgaa cagatccgca gccaagcgca gcgcgaaatt ccggcttggt ataacccgtg
     1861 ggtccacttg tcagcgacga cgggaatcgg agtgctcgcg ctgtgtttgg gaacgtggca
     1921 gcttgccaag cttggtcaga taaagccaag tgatctgctg atcattccga tcacgttcgt
     1981 gctggcaaat ctgttcgagt ggcgggttca caaacatgtg ctgcaccgtc gccgctggcc
     2041 catggaagtg ctgtacgaca agcacacgcc gatgcatcac atgatctatg tggaagacga
     2101 catgacgctg cgcagccagc gcgaatttcg tctggtgctg attccggcag tcggcgtgct
     2161 tggcgtggtg cttgtcacga ctccgtttgc gttaggaatc gctgcgctgt ggtcgacgca
     2221 agctggctgg ctgtttctgg tcacggccag tctgtacatg gtgacgtatg agcttttgca
     2281 tcttgcctat catgcgccca aagacagctt catcggtcgg cgtcgcttca tttctgccat
     2341 gcgcagacac cacgcgcgtc atcacaatcc gcggcttatg cagcgctaca acttcaacgt
     2401 gatcattccg ctgtccgact acttgctggg caccatcgcc cctgcgtctc agcgtcggga
     2461 caatccgcac aaggaggaac catcgtgaat ctgtcgactt tgcacagcgc agcttcctac
     2521 ccgcactctg tttccaaaac cacaacggcc atcacagcgc atttcgacaa gcagagacac
     2581 ccctttcggc gattcctttt cgcttccctt ttgctgtgcg gaatcagcgc cggatgcagt
     2641 ggaacaagtt caaccggcga caatccaagc ccatcccaag atggcgccac gacaaatggc
     2701 agcgacgggg gaaccacagg tggcgcggcg ctttccgatc tgcttccgga agcgctgttc
     2761 aaccaaattt ttctgcaccg cggcacatcc ccctgccctg gcgcgtttta cacgtatcag
     2821 tcttttgtcg atgcggcgcg cgcattccca ggctttgcca cccaaggaac gacggatgat
     2881 cgcaagcgcg agctggcggc atttttggcc aacatttcgc atgaaaccac aggcggctgg
     2941 gcgacggctc cggacggtcc atattcctgg ggtctgtgct tcattcatga aggcggaaac
     3001 atcgatccat cgacgctgac ttcctactgt gtggcaagtg cgcagtatcc gtgcgccccc
     3061 ggaaagaagt actacggacg cggtccgatc cagctaagtt acaactacaa ctatggccag
     3121 gcgggcgctg cgcttggggt cgatctgcta aacaatccgg acctggtcgg gagcgatccg
     3181 gtattggcgt ggaagaccgc gctgtggttt tggatgacac cgcaagcgcc caagccgtcg
     3241 tgtcataacg tgatgatcgg acaatttgcg ccgagcacgc aggatctggc cgcgggtcgc
     3301 aaaccaggat ttggactgac ggtgaacatc atcaacggcg gcgtcgagtg caaccaacca
     3361 acgcccgcgc aagtcaccga tcgcgtcggc ttctacaatc gctacagcca gcttttggga
     3421 gtcagtccag gcaccgatct gtactgcgac aaaatgcaga gctactgatc actctaccga
     3481 tcagtccacc gacggaccat cttgcagttt ccgcgcgtta ggaatcgcga ttaggaatcg
     3541 cgttagggat ttgcagagcc cacaaacgcc gggtcttcca gtgccagcgg ggtgcagctc
     3601 ttgggcgcgg tgacaggatt gcagcgcgca ggcttcccac cgggaacttc gaagtgatag
     3661 gaaaagccac tgacgggctc cggataatcg gtgctgataa agtgtgctcc cgacgaaagt
     3721 gcagcctgtc cacgcgaggt atcggacacc cgcgcttctt tggtatcggc atcagcacga
     3781 ctgcgcacca aaagtcctgc gcgcaccgct ttgccaatgt tcatcacacc cgcgggatct
     3841 tgtggatcat tcaaaaccgc caccgcagca aacgaatccg tcggtgacga atcaacaaac
     3901 agaagtcgtc cgtgcaagtc gcgcagcttg tgggtgtagg ccgtgcgcag cggaccggtg
     3961 gtatcaaatg cgaaaaacac ccgcccacgc agtgtctgca gaagcggcca gccgcgcgtc
     4021 ttcagcgccg caggcagcgt cggaaaatca gcctgaactt catcgggagt gagtatccgt
     4081 tcgcgtggga aagccgccag aatttcccct tccagtttgg caaaaaagga ctccatgtcg
     4141 ctttccggaa cgtcggcttt gggttcgatc tgaacgtaga tgggaaagtg tccgggatac
     4201 gcatcggaaa agcgccgaat ctctgaaaga cagtcggaaa actgcttgca gcgcgtcccg
     4261 gtgtccagaa acggaacgtg ctgcacttgc agtccgtctt gttcgtcgaa aaatacatcc
     4321 agttcgatct gacggattcc cagccgatcg agctgatcat aaagcggtgc atgggtatag
     4381 gcccacgctt ccaccgaacc cgcgtgcgtg tcgacgtgat agctgttgtg ggtgccgcgt
     4441 acctgcacat ccgagagccg cagttctccg tcggaaggat aatggaatgg cttgcccaga
     4501 ttcggctgtt cccgatccgt atcagcagag gaacagccca gcgaaaccag cagcgacagc
     4561 acagacagaa gttgggtacg catgcgcgga gcctacatca aagactctgc tgacttgcgg
     4621 ttttgtcact ggtgctggcg acccgattgc acataggatg caaatccatg tcgtttcagg
     4681 ttgtgctgcg acccgttaca gagtcagacc ttccgacgct gtttgcccat caagatgatc
     4741 cggtggtgcg cgccatggga gtgattccac atcgagccag agaggaattt ttccaaaaat
     4801 ggaaagaagt cctcgcagat acccagttcg cgaagcagtc aatttgggtc gatgaacagc
     4861 tcgtcggaaa catggtctgt tttctgcgcg atggggtcca cgaaatcggc tacgtagtgg
     4921 gtcgcgatca ctggggaaaa ggaatcgcaa gtgcagcgct cgcccagttt ttgccgctga
     4981 tttcccacct gccaaggccc atcactgcgc gcgtggtcaa acacaacttt gcgtcgctgc
     5041 gggtgctgca aaaaaatggc tttttgatcg tggcagagat gacggaaacc gatccacagg
     5101 gaaggcacat tccggaattt cgactgtcgc ttgcagagga accaaaccaa taagcgccgc
     5161 atcgactaag cgccgcatcg acacctaatc ggctgtgatg tttggcccat gggaatgcag
     5221 cgcaagctcg gcagcgacgg accatacgat tgagaggcga agttcccaat cggtgctata
     5281 aacctcggta tgcgtaccat tcggacgttt tcgctgggaa gcttctctgt ttcgtttctc
     5341 gctctcgcgc tcagcgccgt ttggagctgc aacgcgcaga ttggcgattc acccgccgat
     5401 gcgtcggtcg attcggcgat gtggacaccg ccgccgccgc ccgatctccg cttcgatccg
     5461 gatgcgtttt gggcaaatga tccgccgctg caagtctgcg ttccggatgg aggcatgctg
     5521 ccgccgccgc cgactcccgg ggggacgccg gaatgccccg atgacaaaaa ccgccaaggc
     5581 tgcgcatgtc cgacgctggg agaaaaacga tcctgctggc cggggctgcg cgcaaaccgc
     5641 aacttgggca tctgtcgcga cggcgaaacc acttgcgtgc gcgcaggtga gctaggccaa
     5701 gcctggggtc catgtgaagg atacgtcctg cccgtccccg gagcgaccgc cggagccgat
     5761 gcctgcaagt gtttttcggc gggacgctgg gcgctcgaca acttggtgcc gtgcttctac
     5821 acctcaagcg gtcaagacat cggggccact tcgtcggtga ttcagggcgg caaacccacc
     5881 tgcgtgtcga tgagccagcc gctgcaaaaa ccgacgtcgc cgtggagtcc gaacaccctg
     5941 accgtcgatt gcgtgggtca ctggaagctg tgttacacgc tcagagctgg cgacggaaaa
     6001 aatccgctac cgaccgactg cgtggtggcg acaagctgca ccgaaggcga ttacaccgac
     6061 gcaaacaaac cgcaagcgct gccgcagcta ccggcctggg caacgacgac cgcggcccag
     6121 atcgactgcg ccaaaaagtt caaagccacc ggcggctacg gcgaaatgag cgtcgacggc
     6181 accaccgtca cctgcgacaa agtgcagaag gtattcaacc gcgtaaatta ctgcccgctg
     6241 cgctgcaacg acaacccgat gctgccggaa tgccaaaact gcggctcggg cggatcgggt
     6301 ggcttttagc acggacgact cgatcgccta agtaagccaa atcactgcgt ctttccgtcg
     6361 acattgacgg acccccgcgg ctgccctcac cgtaataggg agtgctttcg ctcattgcaa
     6421 tccgtagggg ggtttatggc tacgcgaact ggcgcaggaa aatcggcacc gaaaacgccc
     6481 aaggccacca cggccaaaaa gtcgaccgca accaagaaag cgacgcctgc caaggcgccc
     6541 aaaaaagcgg cggctcctaa gaagactgcg gctcccaaaa agtcggcggc tcccaaaaag
     6601 tcggcggctc ctaagaagac tgcggctccc aaaaagtcgg cggctcccaa ggcggcgact
     6661 tctcgtcggg gggccaaggt cgatctggca gcggcagcgc agaccgcatc cgagatggcc
     6721 aagtccggcg cggtcgtcaa tcacatcgcg tttgtcgtcg accgctcggg ttcgatgtca
     6781 ggcatccgca gcaaggtgat cgcggcgttt aacgcccagc ttgccgagtg tcgcaaaaac
     6841 agcgagtcgc tcgcgcagca gacgtttgtg tcgttttaca cgttccagac caccgtcgat
     6901 ccgccgcgct ttttcgccaa gccgattgcc gatgtgtcgt cgctcaccga gctgcgctgc
     6961 acgggcacga ccgcgcttct cgatgcgacc ggacaggcga ttgtcgatct gtcgagcatg
     7021 ccgtctgccg agctcgacaa cgtgtcgttc ctggtcctgg tgctcaccga cggagcggaa
     7081 aacaactcgc gcaagtacaa gagcacgctc aaagggatga tcgaaaaagt tcaggcgacg
     7141 ggacgctgga cgttcgcgtt tttgacgcca ccgggcgacg aagcaacgct cgctggtttc
     7201 ggcattccgc tcggcaacat ccgcgcgtgg gaaacgacgc aggccggcat caagcagctt
     7261 gggcaggcga tgcagcaggg tctgcagaac ttttaccaag cacgcagcaa aggccaaaaa
     7321 gccattgttg gggtgtttac gaccgatttg tcgacggtat ccacgaccga ggtcaagacc
     7381 acgcttaccg aagcgaccgg cagttttgtc accctgcccg tcgcggcgga ctgcgacatt
     7441 cgtaccctgg tcgaaaaatc gctcggcaat ggcagcttca agaagggcaa cggctactac
     7501 gagctgacca agcccgagct ggtgcaggaa tacaaagaga tcgccatcgt cga
//
LOCUS       DAORGN010000226         7496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4510_length_7496_cov_5.877570, whole genome shotgun sequence.
ACCESSION   DAORGN010000226 DAORGN010000000
VERSION     DAORGN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4510_length_7496_cov_5.877570"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PKL17_18640"
     CDS             complement(<1..945)
                     /locus_tag="PKL17_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46809.1"
                     /translation="MSSSETAAPTLASLVGSQDVARIVRLVRALGTTLAAQISDGKAR
                     LVHPTQVLLKTAPSGEETIDLRESVALSGPESQKDPAIDLLVAYVAPEVLSGKPDAVA
                     STIYHLAGVAYHLLSGEPPFSGSSAAAVRIKVLLEKPVSPRSLRPELSPALDELLLSA
                     LDKKPDARPPSIDDFLNRLAVAATLSVAPEAAQSVQLRAASSPPVIAAGAAVLPVGAR
                     RGRSLALWGLLSLVVVLGFGSLLLLSKSEEQAPAPSAMAPVSSEERRVSERIGGAARV
                     APAPPGGGSASAPGSAMPPPPPPAPAPTQGSAPPGAPKA"
     gene            complement(993..1256)
                     /locus_tag="PKL17_18645"
     CDS             complement(993..1256)
                     /locus_tag="PKL17_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HNK46810.1"
                     /translation="MSAQLLALLSSALSLAVLCSLPVIVVAVLSAFVTAWLQARLGIS
                     DSSLPVAVRLLLGLATLYVAGPYVLTKIVTFATQVWSSLPTLL"
     gene            complement(1253..1870)
                     /locus_tag="PKL17_18650"
     CDS             complement(1253..1870)
                     /locus_tag="PKL17_18650"
                     /inference="COORDINATES: protein motif:HMM:NF013011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46811.1"
                     /translation="MTSTVEFVRAGLPVLLFVFFSLLAYVKVAVVLQVLRRGIGQGVP
                     PLMVTVFLGLSLSALTMLPVVSRSVAQVAAAPDSATSSELWKAGLSPLEQFLMTHTRA
                     ADRAQVSALAERAAQKQGATIESGALPVTLAAFLLGELRTAFQLGFVLLLPFLLIDLL
                     VALLLSGLQLHGLPVQTVTLAFKLLLFVVCDGFSLLYRGLLWGAP"
     gene            complement(1867..2250)
                     /locus_tag="PKL17_18655"
     CDS             complement(1867..2250)
                     /locus_tag="PKL17_18655"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNK46812.1"
                     /translation="MLAQLLLAPWVDSWPVLGLLLRATLVLLLLVAVLILVRRTQRGR
                     ASGMVTLLEKQQLSSTHAVYLVRVLDRKLLLGGGASGLSLLCDLSCDPASHATKTEDQ
                     VDLSPSWLPPVPPKNESSQADGQMP"
     gene            2330..3634
                     /locus_tag="PKL17_18660"
     CDS             2330..3634
                     /locus_tag="PKL17_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46813.1"
                     /translation="MKPLSPIRFPLSIGSTPANLALGICVIGFFGGGPVFFGSLLPDF
                     VAIVCGLLIALWFFGLCMLPFSYRKERASDLVVGPEGIQVFGGRFSTKKLGWSQLAQD
                     GAIALSKTEPSADAPALSELSLGGEVMVRSEESDAHESLAALHESLCTFAEGYLDANM
                     PAQAPVLDPRVLHCPSCGAPQPATNKAHLSCLFCGAQVPVPSALQEAVHSAMSVAEDR
                     VATRKSLQELTRWPRARFVNQVLAIAMFPLALSWPLMAAFTSEFFQYYDILRWRDVFI
                     LFFATCSMSLGMVLFLQSQLVLRRAFALISASFHALPPEDGQSAYRCRSCAAPIVTPE
                     QIEEPLFVCPYCRCDNVILGALLPHTVHTESEQRQSLDALLVERKRTRNRWRLGIVVS
                     VLLIGAGGQVACSAIQRVKQSSLPRHAPVDRTWSYEPGWVKQ"
     gene            3664..4791
                     /locus_tag="PKL17_18665"
     CDS             3664..4791
                     /locus_tag="PKL17_18665"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HNK46814.1"
                     /translation="MKRSTKRSISSLLQICLLLCAASCWQQKPAIPKPDFTLTPALAS
                     QLVRLSLDCVDRPWPNKPGHVLDGETALKAPAQLTPAFFGCFDWHSAVHGHWALARVL
                     GRFPHLPEKDAIRQKLAAHLTPQNIEQERQFFLQERNRTFERPYGMGWLLRLYAELHT
                     LPDPDAKNWESAVAPLGTLMMQRLGEYLGRLTVPVRSGTHDNTAYAMIHALDAARVFQ
                     YEAFEKTLIERARSFYLADRQCPAAYEPSGEDFISPCLVEADLMRRVLPPGEFSAWYR
                     QFLPPTSSPGFATLRTPVAVRDRHDPRIGHLIGLSFQRAAALRGIASTLSFDDRQEGP
                     LLLSASQHAKDGLAQMMDSGYGGSHWLASFALYLFTDVGLP"
     gene            4875..5672
                     /locus_tag="PKL17_18670"
     CDS             4875..5672
                     /locus_tag="PKL17_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46815.1"
                     /translation="MSDDHASLVKKLKDDILHVNDVVASAIESGERQQQYAAGPLIPP
                     LVERYNQLLAQTAESEHHQLERTVGRMLTDLRRHASSLAKQVSGQRAEKAADAGQPFL
                     LSRPQAVSGYREGPRAPRSKPKYAVGGEVEAWCGKCKELRTHDIVAVIDEQPKQVLCT
                     FCKSRHGYRTEPARGAAGDESAAKNVPITTSSRAGGKNDKATKERLQLERELLDAVTA
                     PQFDPRDNFKPGQIIAHPKWGRGKIESVLRGSLLVRFLDGLRQVSRQ"
     gene            5712..>7496
                     /locus_tag="PKL17_18675"
     CDS             5712..>7496
                     /locus_tag="PKL17_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNK46816.1"
                     /translation="MQALGPWLVDAKGRKLLLRGVNLSGSAKVPPGASTHLRQSLQNP
                     ESVSFVGRPLPLDEADEHFARLYALGQHFLRLVVTWEAIEHAGPGIYDRDYLLYLRAL
                     VEKAASHGLLVWIDPHQDVWSRFTGGDGAPAWTLSAVGMVREHLHACGAALLHSEWEG
                     PLPTMIWPTNYVKLGAATMFTLFFAGKDFAPNLFIGDENVQTYLQRHYVAALCEVAKT
                     LRGLPNVVGYGSMNEPSAGFVGASDLTSPLPGMVSKGPVPSPLQAMAAASGIPQEVAV
                     WEFSPSFMGQVGSVKLNPTGLRLWNSPTGDPWRQEGVWDLVEGQPKILRPDHFSQVRG
                     KPVDFAADYLRPFVLYVLSEIRKVSPESLLFFEGPPAPVGHPPPWQASDPGGVVHAGH
                     FYDGMALMGKHYDPDFTIDQSQMMPVFGRDAVRTAYVDQIRALSQHLPDIPFLLGEFG
                     IPFDLDGGASYRSGDFSAQEAALDGYLHALDQNLLSGTLWNYTPDNDNSHGDHWNGED
                     LSIYSRDQRRNTGDIYDGGRALSAVVRPYARATCGTPLEMNFDRTTRRFFFRFRREPS
                     ACADTELFVPRYQYPSGFSVEISAGTYQY"
BASE COUNT         1524 a         2327 c         2223 g         1422 t
ORIGIN      
        1 ggccttgggc gcgcccggtg gtgccgaacc ttgcgtcggg gccggggctg gcggcggcgg
       61 cggcggcatg gccgagcccg gagcggaagc cgacccacca cccggtggcg cgggcgctac
      121 gcgggctgcc ccgcctatgc gctcggaaac acgcctctct tcactagaca ctggcgccat
      181 tgccgacggt gccggtgcct gttcttcgct tttcgacagc agcagcagcg agccaaatcc
      241 caagacgacc acgagcgaaa ggagtcccca cagcgcgagt gaacggcccc gacgagcgcc
      301 gactggcaga acggcagcgc cggcagcgat caccggcgga ctggacgccg cgcgcagctg
      361 cacggattgg gccgcctcgg gtgccacaga caaggtggcc gcgacggcaa gtcggttcaa
      421 aaagtcatca atcgacggcg gacgcgcatc tggctttttg tcgagtgctg atagcaatag
      481 ctcgtcgagc gctggcgata gctcgggccg caggctgcgc ggactgacag gcttttcgag
      541 cagcaccttg atgcgaaccg ccgccgcact gctgccggaa aatggcggtt cgcccgacag
      601 aaggtggtac gcgacgccgg ccagatgata gatcgtgctc gccactgcgt cgggcttgcc
      661 cgatagcacc tcgggcgcga cgtaggccac gagcagatcg atggcagggt ctttctgcga
      721 ttcgggcccc gagagcgcca ccgactcgcg cagatcgatc gtttcttcgc cgcttggtgc
      781 cgtcttcaaa agcacctggg tcggatgaac cagcctggct tttccgtcgg aaatttgtgc
      841 agccagagtc gtccccagcg cacgcacaag ccgcacaatc cgcgcgacat cctgacttcc
      901 gacaagcgaa gccagcgtgg gcgctgcggt ttcgctgctc gacatcaaaa tccccctggc
      961 ccttatcgtg ccacattcgg cacaaaacag cgtcagagca aggtcggcag cgacgaccac
     1021 acctgggtcg caaacgtcac aattttggtc agcacatacg gtccagccac atacagcgtc
     1081 gccaggccca aaagcagccg caccgcgacg ggcagcgacg aatcagaaat tcccagccgc
     1141 gcttgcagcc acgccgtcac aaacgccgac agcaccgcca ccacgatgac cggcaaagag
     1201 cacagcaccg ccagcgacaa cgccgacgac aaaagcgcca gaagttgcgc gctcatgggg
     1261 caccccacag caggccgcga tacagcagcg aaaaaccatc gcagaccaca aacaaaagca
     1321 gcttaaacgc cagcgtcacc gtctgaacgg gcaggccgtg gagctgcaaa ccggacagaa
     1381 gcagcgccac cagcagatcg atcaaaagaa atggcagcag cagcacaaag ccaagctgaa
     1441 acgccgtccg tagctcgccg agcagaaatg ccgccagcgt caccggcagc gcgcctgatt
     1501 cgatggtggc gccctgtttt tgggccgccc gctcggcgag tgcgctgact tgggcacggt
     1561 cggcggcgcg ggtgtgcgtc atcaaaaact gttctaacgg cgaaagaccg gctttccaca
     1621 gctcactgct cgtcgcagaa tccggcgctg ccgccacttg ggcaacactg cgcgaaacaa
     1681 ccggcaacat cgtcagcgcc gaaagcgaaa gacccagaaa caccgtcacc atcagcggcg
     1741 gcacgccctg cccgatgccc cgccggagca cttgcaacac gacggccact ttgacgtagg
     1801 ccaagagcga aaaaaacacg aacagcaaaa ccggcaggcc cgcgcgaaca aactcgactg
     1861 tcgacgtcat ggcatctgcc cgtctgcctg cgaggactcg tttttcggcg gcacaggcgg
     1921 cagccacgac ggggaaaggt cgacttgatc ttctgttttt gtcgcgtgcg atgccgggtc
     1981 acaagacaga tcacacagaa gcgacagacc cgacgctccg ccgcccagaa gcagcttgcg
     2041 atccaaaacg cgcaccagat agaccgcgtg cgtcgacgac agctgctgct tttccagcag
     2101 cgtcaccatc cccgacgcgc gcccgcgctg ggtccgcctg accaagatca gcaccgccac
     2161 caaaagcagc agcaccagcg tcgcccgcaa aagcagcccg agcacaggcc aggagtcaac
     2221 ccaaggcgca agtaaaagct gagccagcat ctttgttcat gagaagcgac ctgcccatct
     2281 tctgtcaagc cagcagccgc ttggagtagt ctgccgctgg acgtttccca tgaagccgct
     2341 ttcgcccatt cgcttcccgc tgagcatcgg ctcgacgcca gccaacttgg cgcttggcat
     2401 ctgcgtcatt ggttttttcg gtggtggacc ggtgtttttc gggtcgctct tgcccgactt
     2461 cgtcgccatt gtgtgcggtc tgctcatcgc gctgtggttt tttgggctgt gtatgctgcc
     2521 gttttcctat cgcaaggaac gcgcgtctga ccttgtggtg ggtccggaag gaatccaggt
     2581 attcggaggc cgcttttcga cgaaaaaact cggctggtcg cagcttgcac aagacggcgc
     2641 catcgcgctt tccaagaccg agccaagcgc cgatgcaccc gcgctttccg agctatcact
     2701 tggcggggaa gtcatggtgc gcagcgaaga aagtgatgcg catgagtcgc tggccgcgct
     2761 gcatgagagc ctctgtacgt ttgcggaagg atatctggat gcaaacatgc ccgcacaggc
     2821 cccggttctc gatccgcggg tgctgcactg tccaagctgc ggcgcgcccc aacccgcgac
     2881 gaacaaagcc catctttcct gcttgttttg tggtgcacag gttcccgttc caagtgcgct
     2941 tcaggaagcg gtgcacagcg caatgtcagt ggccgaagat cgagtggcaa cgcgaaaatc
     3001 gcttcaggaa ctgacccgct ggccgcgcgc gcgttttgta aaccaagtgt tggcgattgc
     3061 gatgtttccg ctcgctttgt cgtggccact gatggcagcg tttacgtcgg agttttttca
     3121 gtactacgac atcttgcgct ggcgtgatgt cttcatcctg tttttcgcca cctgttcgat
     3181 gtctttggga atggttctgt ttctgcaatc gcagctggtt ctccgtcgag cttttgcact
     3241 tatcagcgca tcatttcatg cgcttccacc cgaagacgga cagtctgcct atcgctgccg
     3301 atcctgcgca gctccgattg tgacgcccga gcagatcgag gaaccgctct ttgtctgtcc
     3361 gtactgccgc tgtgacaacg tgatcctcgg cgcgcttttg ccgcataccg tccacacaga
     3421 atccgagcaa agacagtcgc tggacgcact gcttgtcgaa cggaagcgca cacggaatcg
     3481 ctggcgtctg ggaatcgtcg tgtctgtcct gttgatcgga gcaggtggac aggtcgcatg
     3541 ttccgcgatc caacgggtca aacagtcgtc gctgccacgc catgctccag tggatcgcac
     3601 ctggtcttat gaacccggct gggtcaaaca ataaccgcgc gcaaacggac aaatcgggct
     3661 gtcatgaaac gaagcacaaa acgctctatt tcgtcgctgc tgcaaatctg tttgctgctg
     3721 tgtgcggctt cctgctggca gcaaaaaccc gcgattccca aacctgattt cacactcacg
     3781 ccggcccttg cttcgcagct tgtgcggctg tcgctcgatt gtgtggatcg accatggccc
     3841 aacaaaccgg gccacgttct cgacggagaa accgcgctca aagcgcccgc acaactgact
     3901 ccggcattct ttggttgttt cgattggcac tcggctgtgc atggtcactg ggcacttgcg
     3961 cgggtgcttg ggcggttccc acacctgccg gaaaaagacg cgatcagaca gaagctcgcg
     4021 gcccacctta cgccccaaaa catcgaacaa gagcgacagt ttttcctgca agagcgcaac
     4081 cggacctttg aacgtccgta tggaatgggc tggcttctgc gactgtatgc cgagcttcat
     4141 accttgcccg atcccgacgc caaaaactgg gagtcagccg tcgcaccgct cggcacgctg
     4201 atgatgcagc gactgggcga atatctgggc cggctgacgg tgccggtccg ttcgggaacg
     4261 catgacaaca ccgcgtatgc gatgattcat gcgctcgatg ccgcgcgggt gtttcaatat
     4321 gaagcatttg aaaagactct tattgagcgg gcgcgcagct tctatctggc ggaccgtcag
     4381 tgtccggcag cctatgaacc atcgggagaa gacttcatct cgccgtgctt ggtcgaagcc
     4441 gatctgatgc ggcgcgtgct tccgcccggc gagttttccg cgtggtatcg acagtttttg
     4501 ccgcccacaa gctcgccagg ctttgccacg ctgcgaacgc cggttgcggt ccgcgatcgc
     4561 cacgatccgc gcatcggtca cctgatcggt ctttcgttcc agcgcgccgc agccctgcga
     4621 ggaatcgcca gcacactgtc ttttgacgat cggcaagaag ggccgctctt gctctctgcg
     4681 tcacaacacg ccaaagacgg gctcgcccag atgatggatt cgggctacgg cggatcgcac
     4741 tggctggcgt cgtttgcgct gtatcttttt accgacgtgg gactgcccta acctgcggcg
     4801 attgaccggc aagcccgcgc gcagagccga aattggttgt gctatagtgc gcaacccaaa
     4861 cgggagaaat tgtcttgtcc gacgatcacg cttcactcgt caaaaaactc aaggacgata
     4921 tccttcacgt aaacgacgtg gtggccagcg ccattgaaag tggcgaacgt cagcagcagt
     4981 acgctgccgg gccgctcatt ccaccgctcg tcgagcgcta caaccagtta ctggcccaga
     5041 ccgctgaaag cgaacatcac caactggagc gcaccgtcgg tcggatgctg acggatctgc
     5101 gtcgccacgc ttcgtctttg gccaagcagg tgtccggtca gcgcgcggaa aaagccgccg
     5161 atgcgggtca gccttttttg ctgtcgcgtc cacaagcggt cagcggttat cgcgaaggtc
     5221 cgcgggcgcc acgcagcaag cccaagtatg ccgtcggtgg tgaagtcgaa gcctggtgcg
     5281 gcaagtgcaa agagctgcgc acccacgaca tcgttgccgt catcgacgag cagcccaagc
     5341 aagtgctctg tacgttctgc aaaagtcgtc acggctaccg caccgaaccg gcacgcggcg
     5401 cggcgggcga cgagtcagcc gccaaaaacg taccgatcac gacgagcagt cgggcgggcg
     5461 gaaaaaacga caaagcgacc aaagagcggc ttcagctcga gcgtgagctg ctcgatgcag
     5521 tgacggctcc ccagttcgat ccgcgcgaca acttcaaacc gggtcagatc atcgcgcatc
     5581 ccaagtgggg ccgtggcaag atcgaaagcg tgctacgcgg gagcttgctg gtgcgattcc
     5641 tcgacggact gcggcaagtc tcgcgacaat aatcgaaccg accgaaaatc ccctgtgttg
     5701 cggagggagt gatgcaagcg ctcggaccat ggcttgtgga tgccaagggg cggaagctcc
     5761 ttctgcgcgg ggtgaacctg tcgggaagcg cgaaagtgcc gcccggcgcg tcgacgcatc
     5821 tgcgtcagtc gcttcagaac ccggagtcgg tgtcgtttgt cggcaggccg ctgcctctgg
     5881 acgaagccga cgagcacttt gcgcgacttt atgccctggg ccagcacttt ctgcggctcg
     5941 tcgtcacctg ggaagccatc gagcacgccg ggccaggcat atacgatcgc gactaccttt
     6001 tgtatctgcg agcgctcgtc gaaaaggccg catcgcatgg tcttttggtc tggattgatc
     6061 cgcaccagga tgtctggagt cgcttcactg gcggagacgg cgcaccggct tggacgctgt
     6121 cggcggtggg aatggtgcgc gagcatctgc atgcctgtgg cgctgcgctc ttgcattcgg
     6181 aatgggaagg tccgctgccg acgatgatct ggccgacgaa ctacgtcaag ctgggtgcag
     6241 cgacgatgtt cacgctgttt tttgccggca aggactttgc gccaaacctc ttcatcggcg
     6301 acgaaaacgt ccaaacctat cttcagcggc actatgtcgc ggcactttgt gaagtggcca
     6361 agacgctgcg cgggctccca aacgtcgtcg gatacggcag catgaacgaa ccaagcgcgg
     6421 gctttgtcgg cgcaagcgat ctgacaagtc cactgccggg catggtcagc aaagggcccg
     6481 tgccgtcgcc gctgcaagcg atggcggcag cgtcgggaat tccgcaagaa gtcgccgtgt
     6541 gggaattttc gccgtcgttt atgggacagg tcggttcggt gaagctgaat ccgacgggac
     6601 tgcggctgtg gaactcccca acgggcgatc cgtggcgcca ggaaggagtc tgggatctgg
     6661 tggaaggaca gccaaaaatc ctgcgacccg atcacttttc acaggtgcgg ggcaagccgg
     6721 tggattttgc cgccgactat ctgcgaccgt ttgtgctgta cgtgctgtcc gaaatccgca
     6781 aagtcagtcc tgaatcgctg ctgttttttg aaggaccgcc cgcccccgtc ggacatccgc
     6841 caccttggca ggcttccgat ccaggcggag tcgttcatgc gggccatttt tatgacggca
     6901 tggcgctgat gggaaaacac tacgatcccg acttcacgat cgatcagagc cagatgatgc
     6961 cggtgtttgg tcgggatgcc gtgcggaccg cttatgtcga tcagattcgc gccctgtccc
     7021 agcatctgcc cgacattcct tttctgcttg gagagtttgg aattccgttc gatctcgacg
     7081 gaggggcatc gtaccgaagc ggcgattttt ccgcccagga agcggcgctg gatggctatc
     7141 tgcatgcgct tgatcaaaac ctgctatccg gaacgctgtg gaactacacg cccgacaatg
     7201 acaacagcca cggcgaccac tggaacggtg aggatctgtc gatctacagc cgcgatcaaa
     7261 gacgcaatac gggcgacatc tatgacggcg gacgggcgct ctctgccgtc gtcagaccgt
     7321 atgcgcgcgc aacctgcggg actccgcttg aaatgaactt cgatcgcacc acacgccgct
     7381 ttttcttccg cttccgccgc gaaccgagtg cgtgtgcaga caccgaactt ttcgttccgc
     7441 gctaccaata tccaagcggt ttttccgtcg agatatcggc tgggacttac caatat
//
LOCUS       DAORGN010000227         7468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4541_length_7468_cov_5.066640, whole genome shotgun sequence.
ACCESSION   DAORGN010000227 DAORGN010000000
VERSION     DAORGN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4541_length_7468_cov_5.066640"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKL17_18680"
     CDS             <1..764
                     /locus_tag="PKL17_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46817.1"
                     /translation="SFVVWLALFTLVITLVVLAIVLIPKRIRPPQRAQCAVSVEPAGM
                     QVWVDNGAGPIGQTRSKNELMTIELGVGPHRFVLRSPDDGSERSEAIHVNVSEGQPCS
                     LTLKALPSAEPANPPPTVVDAGAAADAAVRPADMATAPVDASAAPVAAPAQPNPAANP
                     APPTTAEADDDAAKKTEKKIRKRPKADKASDLPAPATAPVAQPAQPEATPAADKPEAA
                     KPAEAPKAETPKPEPKAAPKPSTEAAPAETTPPAQ"
     gene            814..1449
                     /locus_tag="PKL17_18685"
     CDS             814..1449
                     /locus_tag="PKL17_18685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNK46818.1"
                     /translation="MELGSSRSVSRFAVAIRVGSFYAAVTLLAIMWGALRGHTNILLL
                     EGSAAGLRLSHLLSSVALGGSMGLLLVLLTRYLQERFDWARTLHNEFRHLLGPMCRRD
                     ILVVAAASAIGEECLFRGALMLHLWQAIPGPTGLLVGVIGSALLFSLLHIGPGSRFVP
                     WTVSALLMGVVLGGLFVLTGDLLAPIAVHFTVNLLNLSDIVRRELPQTALA"
     gene            complement(1441..2535)
                     /locus_tag="PKL17_18690"
     CDS             complement(1441..2535)
                     /locus_tag="PKL17_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNK46819.1"
                     /translation="MEAAKSSSSTDILVLGRMSGVIPAVPAPPKLPTMKALELRAYDG
                     ISLVFVSNKPIPQPGHGEVLVKVQSAPITVHDVLFLQGRYGANRQLPVVPGLECSGIV
                     VQSGGGLLARALLGRRVVCGAPERGDGTWAEYVCVPTWRCVPLRSFMPFDQGATLLST
                     GVAAYALIEAARAKGARAIANTAADTPLGRMLAICAERRGIPMLHLVERATDAAALKA
                     LGARHVVDLSSDVSDAQLVALFQALGVSVVLESRPGERTQQLLQALPTGGTVLMYGQR
                     GEGNCTIDPSELVRHNKSLEGFRLLDWMRKAGFTRSVQAALFAQKQWNLESRGSVPGA
                     ARLPLDSYHQALDLLYRGRITDGQIQFSLS"
     gene            complement(2741..3451)
                     /locus_tag="PKL17_18695"
     CDS             complement(2741..3451)
                     /locus_tag="PKL17_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HNK46820.1"
                     /translation="MKLRRLFPSSAFVLALLLLPLLGCPKAPSQSPAPPPDLDEAPSL
                     GPGQSGSFDYYVLSLSWSPQFCQTRKFPPTDQQCGDGKRFGFIVHGLWPQYEKGYPES
                     CPSTYQLPDATVDKVLRMMPSQKLIRHEWEKHGTCSGLSAEQYFQQVETVFAGLKIPS
                     PYQSPTAPVVTSLAELKKSFLDANPQLAETSITAQCSGVYLREVRICLRKDLSPMPCG
                     GFVRDSCKAPEVQLRPLR"
     gene            complement(3455..4132)
                     /locus_tag="PKL17_18700"
     CDS             complement(3455..4132)
                     /locus_tag="PKL17_18700"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNK46821.1"
                     /translation="MSKPTHEQDGLPQTFDRLPIFPLHRVQLFPRAMLPLYVFEPRYR
                     EMTAACLAGDGLLAIAQLKPGFEKDYQGRPPVRAMAGLGKIIAHKQNPDGTYNILLEG
                     LWRVRIDEELPPDRSYREVIAHRVRDRIAADLNEEQVRETLRSLVDKLARFIGEGGQT
                     LLRLCAETRRLPCLIDVLSSALVRTPALRRRLFECRDLAVRADLLTVSLAKLVAGLNQ
                     GGDSSLN"
     gene            complement(4201..5361)
                     /locus_tag="PKL17_18705"
     CDS             complement(4201..5361)
                     /locus_tag="PKL17_18705"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HNK46822.1"
                     /translation="MTFTRLWLVLLSLVIGGLGVGGLALVRDADQESLRHRDERLTLS
                     QQQIEQALRLDAAQLIETATVIGSDATLASTIEDAERGLGEPRLLQKSLQKRLRRLSD
                     GKKLAFLWLLGRQGQVLARVGLDEDRSGDALDGWPFAELALRGYRLDDLYEQNGRIFT
                     VAGVPLTALSQKGYAGALLIGEPLDEKLGEKLGLQAVWRHRDKLLGESSLRPPADARV
                     VEIRRPGSVDGIALLTWQRAHVVSPFWRLRTLFLALPIGVLLRGAGLSALLLMVGFLL
                     LRLDRSAPTPVPPGLAGLEPSQRPLLQVPTPPVDSFGEPGSLDALPRLYAEFVSTKVR
                     HGESLLGLSFETFCDELRESHARIQVDQGCRDVLFRVHETDGRASLRATPVW"
     gene            complement(5365..6069)
                     /locus_tag="PKL17_18710"
     CDS             complement(5365..6069)
                     /locus_tag="PKL17_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46823.1"
                     /translation="MRLRLLALGIGLALCLPWSAQAAPLRAIVAFSGQPAPSRPLGYF
                     LRPNGILPIAPPSPDPRRETVLEVMPLAGSPKLLPERAGVPPLPTEQTVRVFGQRLLP
                     RQLLLLPGGTLTLRSDDQRPVTVELSPALPGKPSVLLLPGQAQSIRLSSAGELSLSLR
                     EQPSLPVHVLLPLHLATHLEVSEQGTMAVATLDVPPGRYVVRLRVPGGEVWQQEVDVP
                     ADGRELSLKASLGEER"
     gene            complement(6109..>7468)
                     /locus_tag="PKL17_18715"
     CDS             complement(6109..>7468)
                     /locus_tag="PKL17_18715"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNK46824.1"
                     /translation="TDSPYVVANMRIMTRMGQVALDHLETFGGGFVKGLHSLGDLSPD
                     RRFICHFPKRREIWSIGSGYGGNALLGKKCHALRIASTIGRDEGWLAEHMLILGIEDK
                     RGETTYIAAAFPSACGKTNLAMMVPSLGDEFKVTTVGDDICWMHFGPDGRLWAINPEA
                     GFFGVAPGTGKRTNSNALATLRKNSIFTNVALTADNEPWWEGLSPTPPAGLIDWQGRP
                     FNPQSGEKAAHPNSRFTAPIEQCPSVSPKFKAPQGVPISAIVFGGRRAKLAPLVLESR
                     SFQHGVYLGATMASETTAAAVGQVGVVRNDPFAMLPFCGYNMGDYFAHWLNMGQRGTA
                     SSLPKLFQVNWFRTGDDGKFLWPGYGDNIRVLKWIIERVRGEGAAVESPIGLLPQPGA
                     LDTRGLDLPSGAMDQLLSVDVEAWQKEAERSTQFLSKFGAHMPEALWDEHRQLKARLG
                     VA"
BASE COUNT         1479 a         2411 c         2300 g         1278 t
ORIGIN      
        1 tctcgtttgt cgtctggctg gcgctgttta cgctggtgat cacgctggtg gtgctggcga
       61 tcgtgctcat tcccaagcgg attcgtccgc cgcagcgagc gcagtgcgcg gtttccgtcg
      121 agccagcggg catgcaagtc tgggtcgaca atggcgccgg tccgatcggc cagacccgca
      181 gcaaaaatga actgatgacc atcgagctgg gcgtcgggcc acaccgcttt gtgctgcgca
      241 gcccagacga cggcagcgag cgatcggaag cgatccacgt caacgtgagc gaaggccagc
      301 cgtgtagtct gacgctcaaa gcgctgccaa gtgccgagcc agcaaacccg ccgccgacag
      361 tggtcgatgc gggtgcagcg gcggatgctg cggttcgtcc ggcggacatg gcgacggcac
      421 ctgtcgacgc cagcgccgca ccagtcgccg cgccagccca gccaaaccct gccgcaaatc
      481 cagccccgcc cacgacagcg gaagccgacg atgatgcggc caaaaagacg gaaaagaaga
      541 tccgcaagcg gcccaaagcg gacaaggcca gcgatctgcc ggccccagcc accgctcccg
      601 tcgctcagcc ggcccagccc gaagccaccc cggcggcaga caagcctgaa gcggccaagc
      661 ccgccgaagc gcccaaagcc gaaacgccta agccggaacc gaaagccgcg cccaagccgt
      721 ccaccgaggc tgcgcccgcc gaaacaaccc cgcctgcgca gtaagcttca ctgaaaattt
      781 tcctgtgtcc ttggtgccac ttatccttgc accgtggagc ttggttcttc gcgatccgtt
      841 tctcgctttg ccgtcgccat tcgggtgggc tcgttttacg ctgcggtgac gctgctggcg
      901 atcatgtggg gtgccttgcg cgggcacacc aacattttgc tgctggaagg atcggcggct
      961 ggactgcggc tgtcgcacct tttgtcgagc gtggcgctcg gtggatcgat gggtcttttg
     1021 ctcgtgcttt tgacgcgcta cctgcaagag cgcttcgact gggcacggac gctgcacaac
     1081 gaatttcgcc atctgcttgg gccgatgtgt cggcgagaca tcttggtggt ggcggcggct
     1141 tcggccattg gtgaagagtg tctgttccgc ggcgcgctga tgctgcacct gtggcaagcg
     1201 atccccggcc cgaccggact tctcgtcgga gtgatcggct cggcgctgct gttttcgctt
     1261 ttgcacatcg ggcccggcag tcgctttgtg ccgtggaccg tgtcggcgct cttgatgggc
     1321 gtcgtgctcg gtgggttgtt tgtgctcacg ggagatttgc tggcgccgat cgccgttcac
     1381 ttcacggtga accttttgaa cctgagcgac atcgtgcgcc gcgagctgcc gcagacggcg
     1441 ttagcttagc gaaaactgga tctgcccgtc ggtgatgcga ccgcgataaa gcagatcaag
     1501 cgcctggtgg tagctgtcga gcggcagtcg cgccgccccc ggaacactgc cccgcgattc
     1561 cagattccac tgcttctgcg caaacagcgc cgcttggacc gatcgcgtaa agccggcctt
     1621 gcgcatccag tcgagcagac gaaaaccttc gagcgacttg ttgtgccgga ccagctcgct
     1681 tgggtcgatg gtgcagttgc cttcgccgcg ctgaccgtac atgagcacgg ttccgcccgt
     1741 cggcaacgct tgcaaaagct gctgggtgcg ttcgccggga cgggattcca gcaccacgct
     1801 gacgccgagc gcttggaaca gtgcgacaag ctgggcatcc gacacatccg acgacaaatc
     1861 gacgacgtgc cgcgctccga gcgctttgag cgccgccgca tcggttgccc gctcgaccag
     1921 gtgcagcatt ggaatgccac gtcgctcggc acaaatcgcc agcatccgac caagcggagt
     1981 gtccgccgcg gtgttggcaa tcgcccgagc gcctttggcc cgcgctgctt cgatgagtgc
     2041 atacgccgcc acgcccgtgc tcagcagcgt cgcgccctga tcaaatggca taaagctgcg
     2101 cagcggcaca cagcgccacg tcggcacgca gacgtattcg gcccaggtgc catcgccacg
     2161 ctccggcgcg ccacacacca cccgccgacc caaaagcgcc cgcgcaagca ggccgccacc
     2221 gctttgcacc acgatgcccg aacattccag gcccggcacc accggcagct gccgattggc
     2281 accgtagcga ccttgcagaa acagcacgtc gtgcacggtg atcggtgccg actgaacctt
     2341 gacgagcacc tcgccatggc cgggctgcgg aatcggcttg ttgctgacga aaaccagtga
     2401 aattccgtcg taggcgcgca gctccagcgc cttcatcgtg ggcagcttcg gcggggccgg
     2461 caccgccgga atgaccccac tcatccgccc aaggaccaag atgtcggtag aggacgatga
     2521 ttttgcagct tccatagtat ttgtagaatt gccccgttta tataagcagc gcaatagctg
     2581 tcgaaaaatg cagtcgtgtc gacggttcag cgcgtgtcgt ctagaagcgc accaaacagc
     2641 gacgatcgaa ggacacgatg taggcgcagc cgactgtgcc agtttgtcac agtggcgaaa
     2701 ggcgatcagg gcaggatggg cgacgggatg ggcaacgaag ctagcgaagc gggcggagct
     2761 ggacttcggg ggctttgcag gagtcgcgaa caaagccacc acacggcatc ggcgacaggt
     2821 ctttgcgcag gcaaatgcgc acctcgcgca gatagacgcc ggaacactgg gcggtgatgc
     2881 tggtctcggc aagctgcggg tttgcgtcga ggaagctctt tttcagctcg gccaatgacg
     2941 tcacaaccgg tgccgtcggt gactgatacg gcgaagggat cttgagcccg gcgaacaccg
     3001 tctcgacttg ctggaaatac tgctcggcgc tcagcccgct gcaggttccg tgcttttccc
     3061 attcgtgccg aatcagcttc tggctgggca tcatccgcag caccttgtcg acggtggcat
     3121 cggggagctg ataggtcgac gggcacgact cgggatagcc tttttcgtac tgcggccaca
     3181 gcccgtggac gataaagccg aaacgcttgc cgtcgccaca ctgctggtcc gtcggtggaa
     3241 acttgcgcgt ctggcaaaac tgcggcgacc acgacaggct cagcacgtag taatcaaacg
     3301 atccgctctg tcctggccca agcgacggag cttcatcgag atccggggga ggcgcggggc
     3361 tttgggacgg cgctttcgga caaccaagga gcggcagcag caaaagcgcc agcacgaaag
     3421 cagacgacgg aaacaaacga cgtagtttca tgggctaatt aagcgacgag tcgccgccct
     3481 ggttcaagcc agcaaccagt tttgcgagcg acaccgtcag caaatccgcg cgaacggcca
     3541 gatcccggca ctcgaaaagc cgccgccgca gcgctggcgt ccgcaccagc gccgacgaaa
     3601 gtacgtcgat aagacatggc agccggcggg tttcagcgca cagccgcagc aaggtctgac
     3661 cgccctcgcc aataaacctc gccagcttgt cgaccagcga gcgcagcgtt tcgcgcactt
     3721 gctcttcgtt gagatcggca gcgatgcgat cgcgaacccg atgggcgatc acttcgcggt
     3781 acgagcgatc cggcggcagc tcttcgtcga tgcgaacgcg ccagagtcct tcgaggagaa
     3841 tgttgtacgt tccgtcggga ttttgcttgt gcgcaatgat cttgccgagc ccagccatcg
     3901 cccgcaccgg aggccgccct tggtagtctt tttcaaaccc cggtttgagc tgcgctatcg
     3961 ccaaaagccc gtcgccggca aggcacgcgg cggtcatctc acggtagcgc ggttcaaaga
     4021 catacaaagg cagcatcgcg cgcgggaaca gctggacccg atgcagcggg aaaatcggca
     4081 accgatcgaa cgtctggggg agaccgtcct gctcgtgcgt gggcttgctc atcgggaagc
     4141 tctcccatgg aagatgccgc gcagcttcaa actgcgcccg cgtacagcaa agagcggcaa
     4201 ctaccacacc ggtgtcgcgc gaaggctcgc acgaccgtct gtttcgtgga cgcgaaagag
     4261 cacgtcgcgg catccttgat cgacttggat gcgggcgtgg ctttcgcgca gctcgtcgca
     4321 aaatgtctcg aaactgagcc cgagtagtga ttcgccgtga cggactttgg tcgacacaaa
     4381 ctcagcgtaa agccgtggca gcgcgtcgag tgaaccgggc tcgccaaagc tgtcgactgg
     4441 cggtgtgggc acttgcagaa gcggacgctg cgacggttcg agaccggcca aaccaggcgg
     4501 caccggcgtc ggcgcggagc gatcgagacg cagcaacaag aaaccgacca tcaacagcag
     4561 cgccgaaagc ccggctccgc gcagcaaaac cccgatcggc agcgccaaaa aaagcgtgcg
     4621 caggcgccaa aagggcgaca cgacatgtgc ccgctgccaa gtcaaaagag cgatcccatc
     4681 gacagaaccg ggacgacgaa tttcgacaac cctggcatcg gcaggcggac ggagcgacga
     4741 ttccccaagc agcttatcgc gatgccgcca gaccgcctgg agcccgagct tttcgccgag
     4801 cttttcgtcg agcggttcac cgataagcag cgctccggca tagccctttt gcgaaagcgc
     4861 ggtcagcggc acaccagcga cggtaaaaat ccgcccgttt tgttcgtaca ggtcatcgag
     4921 ccggtagccg cgcagcgcaa gctctgcaaa tggccagcca tcgagcgcgt caccactgcg
     4981 atcttcgtcg agacccaccc gcgcaagcac ctgaccttgc cgcccgagca gccacaaaaa
     5041 cgccagcttt tttccatcgg agagccgtcg cagacgtttt tgcagcgact tttgcaaaag
     5101 ccgcggttcg ccaagtccgc gctcggcatc ttctatcgtg ctggccagcg tcgcatcact
     5161 gccaatcacc gtcgccgttt cgatgagctg tgcggcgtct agtcgcagcg cctgttcgat
     5221 ttgctgctgg ctcagcgtca gtcgctcgtc tctgtgccgc agcgattcct gatccgcatc
     5281 gcgcacaagc gcaagcccgc cgaccccaag cccaccgatg acaagcgaga gcagcacaag
     5341 ccaaagccgc gtaaaagtca tcggctagcg ctcctctccg agactggctt tgagcgacag
     5401 ctcccgtccg tccgctggca catcgacttc ttgctgccac acttcgccgc ccggcacccg
     5461 cagccgcacc acgtaacgtc ccggcggcac atcgagcgtc gccaccgcca tcgtcccctg
     5521 ctcgctgacc tccagatgag tcgccaggtg cagaggcaaa agcacatgca caggcaaaga
     5581 cggctgctcg cgcagagaca gcgacagctc acccgcagac gaaagacgga tcgactgggc
     5641 ttggccgggc agaagcagga cagacggctt ccccggaagc gctggcgaaa gctcgacggt
     5701 cacaggacgc tggtcatcgc ttcgcagcgt gagtgtaccg ccgggcaaaa gcaacagctg
     5761 ccgaggcaac agccgctggc cgaacacgcg caccgtctgt tccgtcggca gcggcggtac
     5821 accggcccgt tccggcaaga gcttgggcga cccagcaagc ggcatcactt ccagcacggt
     5881 ttcccgtcga ggatcaggcg atggcggcgc gatcggcaag atgccattgg gccgcaaaaa
     5941 atagccgaga ggccgcgacg gcgcaggctg ccccgaaaaa gcgacaatcg cgcgcagcgg
     6001 cgccgcttgc gcggaccacg ggagacaaag cgcaaggccg attcccagcg caagcaagcg
     6061 aagacgcatc gacggaaatc cggtcacaac gggcccgcgg ttgtgcggtt acgccacgcc
     6121 aagtcgggcc ttgagctgac ggtgttcgtc ccacagcgct tcgggcatgt gcgcaccgaa
     6181 cttgctgagg aactgcgtgc tgcgctctgc ctccttctgc cacgcctcaa catcgacgga
     6241 cagcagctga tccatggcgc cactcggcag atccagccca cgggtatcca gcgcgcctgg
     6301 ttgtggcaaa agcccaatcg gcgattcgac cgccgcgcct tcgccacgca cccgctcgat
     6361 gatccacttg agcacgcgga tgttgtcgcc atagcccggc cacaggaact tgccatcgtc
     6421 gccggtacgg aaccagttga cctggaacag cttcggcaag ctgctcgcgg tcccgcgctg
     6481 acccatgttg agccagtggg caaagtaatc gcccatgttg tagccacaga acggcagcat
     6541 cgcgaatgga tcgttgcgca cgacacccac ctggccgact gctgccgccg tggtttccga
     6601 cgccatcgtc gcgcccaggt agacaccgtg ctggaagctg cgtgattcga gcaccagcgg
     6661 tgccagcttg gcccgtcgtc caccaaaaac aatggccgaa atcggcacac cttggggcgc
     6721 tttgaacttc ggcgagaccg acggacactg ctctatcggg gcggtgaagc gcgagttcgg
     6781 atgcgctgct ttttccccac tctgcggatt gaaaggacga ccctgccagt cgataagacc
     6841 tgccggtggt gttggactaa gtccttccca ccacggctcg ttgtcggcgg tcagcgcgac
     6901 gttggtaaag atgctgtttt tgcgcagggt cgcgagcgcg ttggagttgg tgcgcttgcc
     6961 ggtgcccggt gcgacgccaa aaaagccagc ttcgggattt atcgcccaca gcctgccgtc
     7021 gggaccaaag tgcatccagc agatgtcgtc accgaccgtg gtgactttga actcgtcgcc
     7081 cagcgaaggc accatcatcg ccaggttggt cttgccgcac gccgacggga acgccgccgc
     7141 gatgtatgtg gtttcgccgc gcttgtcttc gatgccgagg atcagcatgt gctcggcgag
     7201 ccagccttcg tcgcgaccga tggtgctggc gatgcgcagc gcgtgacatt ttttaccgag
     7261 cagggcgttg ccgccgtaac ccgatccgat gctccaaatc tcgcgccgct tggggaaatg
     7321 gcagatgaag cgacgatccg gggacagatc gccgagcgag tggagccctt tgacaaagcc
     7381 gccgccaaac gtctccaagt gatcgagcgc gacctgaccc atgcgggtca tgatgcgcat
     7441 gttggcgacg acatacggac tgtcggtg
//
LOCUS       DAORGN010000228         7442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4566_length_7442_cov_5.586165, whole genome shotgun sequence.
ACCESSION   DAORGN010000228 DAORGN010000000
VERSION     DAORGN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4566_length_7442_cov_5.586165"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="PKL17_18720"
     CDS             <1..1989
                     /locus_tag="PKL17_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK46825.1"
                     /translation="ATAAATIYTITRDEILQRGYTTVADALASVPGLYVIDDLVMPAL
                     SVRGVSGGLYAGTRLCKLMIDGNTVAFRPEQSAFLGLEFIPIDAIERIEVAKGPLSAV
                     YGANAFLATINVITRRSQEGVIGALTANFTYLRSPGVSGSALVSYASSRGYVMAAVGG
                     GRLDRSGLSVPQTLPDTAAQGNIDYGKATQGDISQPLSMALSAGLRSEKLGELSLSTG
                     LQRLDSNGLFRINSLLADGNRIALANIWSGLRYEKKWTKAALNLSTSLNHGEPLREYE
                     LLLAGNSNYSFKPNYDYTSSTSSAEVSYSPLGQKLSLRAGGEFEYARERVAYYTQIFR
                     RQEGTNQPGDTVDLIEGHDRDRDYFGGAGYLSVTSAPFTRRLKGLRISGDLRVDKIQF
                     GPITYDPQWSWRFAIAYRINPRIVAKLVSGRSFQMPSGTLLFSEAGFGNRNNIIGAYE
                     FNSTRPLLPQHIYNIELSLSAVIFKHWALEASLFDQSLVDKVEFVSVGGDFVAKNRNP
                     QSVIGFELLSRVLFGRFTAYGWLSGVYSVAAFGAAAVSNPLPAFPSLQGILGIDLDVP
                     EAKLHLNAQARLVGPRGSSPSNTLLNNDEPYTLPAYGVLDVTLSTQALHPFGLTADTR
                     LLLTARNLGPKRWAEPGHAGFDLPALGPSLHLALKQSF"
     gene            1999..2376
                     /locus_tag="PKL17_18725"
     CDS             1999..2376
                     /locus_tag="PKL17_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46826.1"
                     /translation="MKNGRKTILLVDDAMTIRKLARAMLGADFDFLEAENGEQAHVVA
                     KQSHPDLIVMDLNMPVKDGIDGLADLKRDPSTSRIPVIILTSETEPHKRERCLDLGCA
                     DFLRKPLDRTQLKNAVTRNLAPR"
     gene            2373..2849
                     /locus_tag="PKL17_18730"
     CDS             2373..2849
                     /locus_tag="PKL17_18730"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK46827.1"
                     /translation="MKHGTLIISCAQALLALPVQHIVEVSRMVAPCARLLRVPKFVLG
                     IVDFHGQLVPVLDLPARLGLCPARSVLDLALGHIVFIELPGGIWGLAVDNVQDLIELP
                     TEPIAFEPRHLAGLLLGSIKLPSHERALVLNPVKLLSVVTRAKVASELRELLGGEA"
     gene            2846..4156
                     /locus_tag="PKL17_18735"
     CDS             2846..4156
                     /locus_tag="PKL17_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNK46828.1"
                     /translation="MMSLAEQLARRYGLHFAGHNVDLLEHGLRRLADSLGLSLDVLCL
                     RLADRDASRELWQKAIEFVTINETYLFRHPEQLALLGQRLIPERLATGQRHLRVWSAG
                     CATGEEAYSIAMVLRHAAPQIDLSVLATDISQSVLAVAAKGRYGSNSLRETIPPHLAS
                     AYLLPTADGGVEISPEIRSIVELRQVNLVDSDDLATVPGGFDFIFCRNVLIYFTHEHN
                     RDVLKLLRSRLVDDGVLILSALDQREKVPGLELQVIDGVPILRRVGVARTMGNPASPR
                     RDSSPLRELLALPTPPPRPLLPSIADDTRERQAEVKAAADSGDLQRASQLARRLIQSD
                     RTPQSLHLLAMILGEQGKHSEAEQLLLEALNQNPDYVLAHLSLGLMERPQHQRWRGAQ
                     HLRTVMTLLAQRPDDEVLNGPEPLQVAMARRLATAGLANMERRS"
     gene            4153..4524
                     /locus_tag="PKL17_18740"
     CDS             4153..4524
                     /locus_tag="PKL17_18740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46829.1"
                     /translation="MKKVLIVDDSATTRAVVKVYLSGQSLLFLEADNGTDAMRIALDE
                     RPDAIIIDLKMPGMDGLSFIRRARMSIALQKTPIILITGQKSPEIKAEAMRCGASHFM
                     TKPIDVPVMAKLIMKSVGLEK"
     gene            4528..5046
                     /locus_tag="PKL17_18745"
     CDS             4528..5046
                     /locus_tag="PKL17_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNK46830.1"
                     /translation="MSDAGVENLELLLHERAAQLSRRARGEEESALLPHVAFQSAGEQ
                     FAVPLRYVVHASRLRHLTPIPGGPSYLLGICALAGHLVSVLDVAAFLELRHRGLQDVA
                     CCLVVSVDGRELGLCAERLLGIEDLPATSILPWASVHGAVGQIATLETQRFLILDLQK
                     LLLDPRLSEEKH"
     gene            5050..7257
                     /locus_tag="PKL17_18750"
     CDS             5050..7257
                     /locus_tag="PKL17_18750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46831.1"
                     /translation="MDELLREVIDIFVAEVREQADRIAQALLLMEQQPSKIPTEIEEL
                     YRQAHSMKGSSASLGVEELSLLAHHFESALLPVRRHRAPLTAEMVDLGLKAMDAAKLR
                     VVGLQADSDVGLAEVRALTDRLERLAAEQPSASPSGAATEAREVPVPLVSVDEVGEPV
                     QSPQESDPTATHAPATDAPDTVRVSIDKLAQLVQHTDDLRRLRGQLERHHTDVTTLAR
                     NVERLLRHASKGGEPLSRKTTEELSQFLRGLRALRRDLLDDSEALQATTSELDDGLRA
                     MQLLPATLLQQPLHRAVREACRATGKEAALRLFGEDVFLDRTLLEAIKTPLLHLVRNS
                     VDHGIEDVAVREAIGKPALGRIAVNIEQRGSQVEIAVTDDGRGLDVARVREVAVQKGL
                     LSAAEANRLDENESYALLLRPGFSTKEEATELSGRGVGLDVVNTTVRKLHGSLQMSSQ
                     PGQGTTFLISVPLTVAASRLLLLDEQDHTFALPLPAISRIALPRREELVKVGNQTVYY
                     IDDQPHTVIGLSKLLGLTYSGEAPSRQPLLLLRGTGGVAIACDRLRGENDLVLRPLPV
                     ELRRLSLLSAVAIVPSGPALFVLSPQALISAAASLRKVQDVRSRRQTVLVADDSITTR
                     SLLRSVLESGGYRVRTAADGDEALRLARGEPIDLLVSDVRMPRLDGISLLAKLRSDAR
                     TSKLPVVLFSGANNEEDRQRGLAAGASAYLAKSDFERGHLIDVVTQLLGESAS"
     gene            7254..>7442
                     /locus_tag="PKL17_18755"
     CDS             7254..>7442
                     /locus_tag="PKL17_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK46832.1"
                     /translation="MSQIDENRICRVLLVDDSDSYLRALTKLLSREKDIVVVGEAQDG
                     RAAVEQAAKLRPDVIIMDI"
BASE COUNT         1388 a         2226 c         2378 g         1450 t
ORIGIN      
        1 gccacggcag cagcgacgat ttacaccatc acccgtgacg agattttgca gcgcggttac
       61 acgacggtgg ccgatgcgct ggcgagcgtg ccggggctct atgtcatcga cgatttggtg
      121 atgccggctc tgtcggtgcg cggggtgagc ggcgggctgt atgcgggcac ccgtctgtgc
      181 aagctgatga tcgacggaaa caccgtcgca tttcgccccg aacagtcggc cttcttggga
      241 ctggaattca ttcccatcga cgcaattgag cgcatcgagg tcgccaaagg cccactttca
      301 gcggtgtacg gcgcgaacgc ttttctcgcg acgatcaacg tcattacgcg ccggagccag
      361 gagggcgtca tcggcgcgct gaccgccaat ttcacttatc tgcgcagccc aggcgtaagc
      421 ggttccgcgc ttgtcagcta cgccagcagt cgtggctacg tcatggcagc cgttggaggc
      481 ggacggctgg accgatcggg gctttcggtg ccgcagacgc ttcccgatac cgcggcgcag
      541 ggcaacatcg actatggcaa agcgacccaa ggggacatct cgcagccgtt gtcgatggcg
      601 ctgagcgcgg gactgcgcag cgaaaagctt ggcgagctgt cgctgtccac ggggttgcaa
      661 aggctcgatt caaacggctt gtttcgcatc aacagcctgc tggccgacgg aaaccgcatc
      721 gcacttgcga acatttggag cggcctgcgc tacgaaaaga agtggaccaa ggcggcgctc
      781 aacttgtcga cgtcgctcaa tcacggcgag ccactgcgtg aatacgagct tttgctggct
      841 ggcaacagca actacagctt caagccgaac tacgactaca catcttcgac gagcagcgcc
      901 gaagtaagct acagcccgct tgggcaaaaa ctgtcgctgc gcgccggcgg agagtttgaa
      961 tatgcacgcg agcgcgttgc gtattacacc cagatttttc gtcgccaaga aggcaccaat
     1021 cagccgggtg acaccgttga tctaatcgaa ggtcacgacc gcgatcgcga ttacttcggc
     1081 ggcgccggat atctgtccgt caccagcgcg ccgtttacgc gccggctcaa gggtctgcga
     1141 atcagtggcg acttgcgcgt cgacaagatc cagtttggcc cgatcactta cgatccgcag
     1201 tggagctggc gcttcgcgat tgcctatcgg ataaaccctc gcatcgtcgc caagctggtc
     1261 agcgggcgat cctttcagat gccgtcgggg acgctcttgt tttccgaagc tggatttggc
     1321 aaccgcaaca acatcatcgg cgcttacgag ttcaacagca cgcgaccgct tctgccgcag
     1381 cacatctaca acatcgagct gtctctgtcg gcggtcatct tcaaacactg ggcgctcgaa
     1441 gcgtcgctgt ttgatcagtc gctcgtcgac aaagtcgagt ttgtgtccgt cggtggggac
     1501 tttgtcgcca aaaaccgcaa tccgcagagc gtcatcggtt tcgagctgtt gtcgcgggtg
     1561 ctgtttgggc gctttaccgc ttacggttgg ctgagcgggg tgtacagcgt cgctgcattt
     1621 ggggcggcgg cggtgagcaa tccactgccg gcgtttccga gcctgcaagg cattttgggt
     1681 atcgatctcg acgtgcccga agccaagctt catctaaatg cccaggcgcg tctggtcggg
     1741 ccgcggggtt cgtcgccgag caatacgctg ctcaacaacg acgagcctta cacgctgccc
     1801 gcgtatggcg tgctggatgt cacgctgtct acgcaggcgc tgcatccgtt tggtctaacg
     1861 gcggacaccc ggctgctctt gactgcgcgc aacctggggc caaagcgctg ggctgaaccg
     1921 gggcacgccg gctttgatct tcccgccttg gggccaagtc tgcatctggc cctcaagcag
     1981 agtttttagc gagggagcat gaaaaacggt cgcaaaacca tcctgcttgt cgacgacgcg
     2041 atgacgattc gcaagctggc gcgggcgatg ctgggtgctg actttgactt cctcgaagcg
     2101 gaaaatggcg agcaggcgca cgtcgtcgcc aagcagagcc atcccgacct catcgtgatg
     2161 gatctgaaca tgccggtcaa agatggcatc gatgggctgg ccgatctcaa gcgcgatccg
     2221 tcgacgagcc gcatccctgt catcattttg acgtcggaaa ccgagccgca caaacgcgag
     2281 cgctgtctcg atttgggttg tgccgatttt ctgcgcaagc cactcgatcg cacccagctc
     2341 aaaaacgccg tgacgaggaa cctggcaccc cgatgaagca cggcaccctg atcataagct
     2401 gtgctcaggc gctgcttgcg ctgcccgtcc agcacatcgt cgaggtgtcg cggatggtgg
     2461 cgccgtgcgc acggcttctg cgggtgccca agtttgtgct cggcatcgtc gattttcacg
     2521 gtcagcttgt gccggtgctc gacctgccgg cgcggctggg tttgtgtccg gcgcggagcg
     2581 tgctggattt ggcgcttggg cacattgttt ttatcgagct gcccggcgga atctggggtc
     2641 tggccgtcga caatgttcag gatctgatcg agctgccgac cgagccaatc gccttcgagc
     2701 cgcgccatct ggcgggtctt ttgctcggtt cgatcaagct gccgagccac gagcgggcgc
     2761 tggtgctaaa tccggtgaaa ctgctgtcgg tggtgacgcg ggccaaggtg gcaagcgagc
     2821 tgcgcgagct gttggggggc gaggcatgat gtcgctcgcc gaacagctcg cgaggcgcta
     2881 tgggcttcac tttgcgggcc acaatgtcga tctgctcgaa catggtctgc gccggctggc
     2941 cgattcgctg gggctgtctc tcgacgtgct gtgtcttcgg ctggccgatc gcgacgcgag
     3001 ccgcgagctt tggcaaaaag cgattgagtt tgtcacgatc aatgaaacct atctgtttcg
     3061 tcaccccgaa cagcttgcgc tccttgggca gcggctgatt ccggaacgac tggcgaccgg
     3121 gcagcgccac ctgcgggttt ggagtgctgg ctgtgcgacg ggtgaagagg cgtattcgat
     3181 cgcgatggtg ctgcgacatg cggcgccgca gatcgatcta agcgtccttg ccaccgacat
     3241 cagtcaatcg gtgctcgccg tcgctgcgaa aggtcggtat ggatcgaact cgctgcgaga
     3301 gacgatcccg ccgcatttgg ccagtgcgta tcttttgcca acggcagacg gcggcgtcga
     3361 aatttcgcct gagattcgct cgatcgtcga gctgcgacaa gtgaacttgg tggacagcga
     3421 cgatttggcg acggtgccag gcggcttcga ttttatcttt tgccgcaatg tgctgattta
     3481 ctttacgcac gagcacaacc gcgacgtgct gaagctgctg cgcagtcggc ttgtcgacga
     3541 cggggtgctg attttgtcgg cgctggatca gcgcgaaaaa gtgccggggc tcgaactgca
     3601 agtgatcgac ggtgtgccga tccttcgtcg ggtcggagtg gcgcgcacca tggggaatcc
     3661 cgcgtcacca cggcgagaca gctcaccgct gcgcgagctg ttggctttgc cgacaccacc
     3721 gccgcggcct ctgctcccgt cgattgcgga tgacacccga gagcgccaag ccgaggtcaa
     3781 agccgccgcc gacagcggtg atttgcagcg ggcgtcccag cttgcccgtc gactcatcca
     3841 gtcggataga acgccgcagt cgctgcatct tttggcgatg atcctcggtg aacagggcaa
     3901 gcacagcgag gcagaacagc ttctcctcga agcgctgaat caaaatccag actatgtgct
     3961 ggcgcacctg agcctggggc ttatggaaag gccgcagcat cagaggtggc gtggcgcgca
     4021 gcaccttcgg accgtgatga cgctgcttgc acagcggccc gacgatgaag ttttgaatgg
     4081 acctgagccg ctgcaagtcg cgatggcgcg tcgactcgca accgccgggc tggccaacat
     4141 ggagagacga tcgtgaaaaa agtgctcatc gtggatgact ccgccaccac tcgggccgtc
     4201 gtcaaagtct acctaagcgg ccagtcgctc ctgtttttgg aagccgacaa cggcactgat
     4261 gcgatgcgga tcgctctcga cgaaagacct gacgccatca tcatcgacct caagatgccg
     4321 ggcatggatg gtctgtcgtt tatccgtcgc gcccgcatga gcatcgcgct ccagaaaacc
     4381 cccatcatcc tgatcaccgg gcaaaagtct cccgagatca aagccgaggc gatgcgctgc
     4441 ggtgcgagcc actttatgac caagcccatc gacgtgccgg tgatggccaa gctcatcatg
     4501 aagtcggtcg ggctggagaa gtagcagatg agcgacgccg gcgtggaaaa cctggagctg
     4561 ctcctgcacg agcgggcggc gcagctgtcg cgacgcgcac ggggcgaaga ggaatcggcg
     4621 cttttgccgc acgttgcgtt tcagtcggcg ggagaacagt ttgcggtgcc gctgcgctac
     4681 gtggtccacg ccagccggct gcgtcacctt acgccgatac cgggcggtcc gtcgtatctt
     4741 ttgggaatct gcgcgctggc tgggcatttg gtgtcggtgc tcgacgtcgc ggcgtttttg
     4801 gaactgcgcc atcgcgggct ccaagacgtg gcctgctgtc tggtcgtcag cgtcgatggc
     4861 cgcgagctgg ggttgtgcgc cgagcgcttg cttgggattg aagacttgcc agcgacgtcg
     4921 attctgccgt gggccagcgt gcatggtgca gtggggcaga ttgccaccct cgaaacccag
     4981 cggtttttga tcctcgattt gcagaagctg ctgctcgacc ctcgcctgtc cgaggagaag
     5041 cactagccca tggatgaact gctccgcgaa gtcatcgaca ttttcgtcgc tgaagtacgc
     5101 gagcaggcgg atcgcattgc ccaagcgctg ctgcttatgg aacagcagcc gagcaaaatt
     5161 ccgacggaaa tcgaggaact gtatcggcaa gcgcacagca tgaaaggctc gtcggcgtct
     5221 cttggggtcg aggagctgtc gctgttggcc caccacttcg agagcgcgct actgccggtg
     5281 cggcgtcatc gcgcgccgct caccgccgag atggtcgatc tgggactgaa ggcgatggat
     5341 gcggccaagc tgcgcgtggt cggactgcaa gccgatagcg acgtcggact tgccgaagta
     5401 cgggcgctaa ccgatcgcct cgaacggctg gccgcagagc agccaagcgc cagtccatcc
     5461 ggtgcagcga cggaagcgcg cgaagtgccg gtgccgcttg tttccgtcga tgaagtcggc
     5521 gaaccagtcc aatcgccgca agaaagcgat cccacagcga cccacgcgcc agcgaccgac
     5581 gcacccgaca cggtgcgggt gtcaatcgac aagctcgctc aactggtgca gcacaccgac
     5641 gatttgcggc gactgcgggg tcagctggaa cggcaccata ccgacgtgac gacgcttgcg
     5701 cgcaatgtcg agcggctgct ccgtcacgca agcaagggcg gcgagccgct cagccgcaaa
     5761 accaccgagg agctgtcgca gtttttgcgc ggtctgcggg cactgcggcg cgacctgctc
     5821 gacgacagcg aagcgctgca agcgaccaca agcgagctcg acgacggact gcgggcgatg
     5881 cagcttctgc cggcgacact gctgcagcag ccactgcatc gagcggtgcg tgaagcctgc
     5941 cgcgcgacgg gcaaagaagc ggcgctgcga ctgtttggcg aagacgtatt tcttgatcgc
     6001 acgctgctcg aagcgatcaa gacgccactg ctgcacttgg tgcgaaactc cgtcgaccac
     6061 ggtatcgaag atgtcgctgt gcgcgaagcg ataggcaagc cggcgctggg tcgaattgcg
     6121 gtgaacatcg agcagcgcgg cagccaagtc gagatcgctg tcaccgacga tgggcgtggt
     6181 ctggatgtgg cgcgcgtgcg cgaagtggcg gtgcaaaaag gcctgctgtc tgctgcggag
     6241 gccaatcggc tcgacgaaaa cgagtcgtat gcgctgcttt tgcgtcccgg tttttcgacc
     6301 aaagaggaag ccaccgagct gtcgggacgc ggcgtcggtc ttgatgtcgt caatacgacg
     6361 gtgcgcaagc tgcatggctc gctccagatg tcgtcgcagc cgggtcaagg caccacgttt
     6421 ttgatctcgg ttccgctcac cgtcgcggcc tcgcggctgc ttttgctcga tgaacaagac
     6481 cacacctttg cgttgccgtt gccagcgatc agccggattg cgctgccgcg acgcgaagaa
     6541 ctggtcaaag tgggcaatca aaccgtttat tacatcgacg accagccgca tacggtgatt
     6601 ggtctttcga agctgcttgg actgacgtac agcggcgaag caccgagccg acagccgctg
     6661 ctgctcctac gtggcacggg cggtgtggcg attgcctgcg atcggctgcg cggcgaaaat
     6721 gacttggtgt tgcgaccgct gcccgtcgag ctgcggcggc tgtccctgct atcggcggtg
     6781 gcgattgtgc ccagcggacc ggcgctgttt gtgctgtcgc cccaagcgct gatttctgcg
     6841 gctgcgagtc tgcgcaaggt tcaggatgtg cgcagccgtc gacaaaccgt gctcgttgcg
     6901 gacgattcga tcaccacgcg atcgcttctg cgcagtgtgc tggaaagcgg cggctatcgg
     6961 gtgcgcacgg ctgccgacgg tgacgaagcg ctgcgacttg cccgtggcga accgattgat
     7021 ctgctcgtgt ccgacgtgcg gatgccgcgg ctcgacggaa tttccctgct ggccaagctg
     7081 cgcagcgacg cccgcaccag caagctcccc gtggtcctgt tttccggcgc caacaacgaa
     7141 gaagatcgcc agcgcggcct ggctgctggt gccagcgcct atctcgccaa gagcgatttc
     7201 gagcgcggcc acttgatcga cgtcgtcacc cagctgcttg gggagtccgc atcatgagcc
     7261 agatcgacga aaatcgcatc tgccgcgtgc tgctggtcga cgacagcgat tcctatctgc
     7321 gcgcgctgac caagctgcta agccgcgaaa aagacatcgt cgtcgttggt gaagcccaag
     7381 acggcagagc cgccgtcgag caagccgcaa aacttcgtcc cgacgtcatc atcatggaca
     7441 tc
//
LOCUS       DAORGN010000229         7429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4577_length_7429_cov_5.420938, whole genome shotgun sequence.
ACCESSION   DAORGN010000229 DAORGN010000000
VERSION     DAORGN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4577_length_7429_cov_5.420938"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PKL17_18760"
     CDS             complement(<1..1500)
                     /locus_tag="PKL17_18760"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46833.1"
                     /translation="MSQADSATPALRPGDLVSARYRVERFLDRIGAEESYAVRAVEDG
                     RPLLLKLLIWPGGPPELPQDELLDWKSGVQRLASFRHPSLSQTHALIPFDEEGRTCLL
                     REPVELEGKTLPELLRSLGRPLRETEVQDLASQIGEALDAVHRLDLLHLGLSPRRIVV
                     ISDGDNWRVKLTDVGLYPKTLAARFAEPGYLAPEVLSGQACDPRSDQFSLAVILYELL
                     AGQPAFVGAPDEPREVVVQRVLAEDPLPLLLSKRVELALQRALSRSRGVRFAHLRDFV
                     AALGGSVSRFSPPTEGRVAALPRRPESTWPRLWLPMVQGALWAVCGLGLLLGARKLLF
                     PKKPRTQPPQIVAAPSIETETPDAGSPLAPDAGMEVLLAKLPPLRSASDTPIAAKTAT
                     ATTTKPTSPQIPKPQGPRPQQIRRDGGIGAGDELPPPPTVDTPDPAASVQVDVSSQDG
                     KLTAVQESKLRACLRLVRRPAPPYRVVLENISGTLYVSPRGTSDEYQTSS"
     gene            complement(1510..2724)
                     /locus_tag="PKL17_18765"
     CDS             complement(1510..2724)
                     /locus_tag="PKL17_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46834.1"
                     /translation="MLPNFRALGSSRESGSLRFFRPGMWWLLAALIGFSSLPAHAAPL
                     ILVLGIKREGQSSAKSRSVVAQHLLRMGEPAVSPSLQSSELLCTEKPCLTRLGKAFSA
                     RRLIGGEVFPNDRSYLVRLWLYDLDAEQPTLVEERCTDCSEELLQESVSRAAGRLVDA
                     LAAQAALVPVAPAPSAPSPQLVPVPVAPPPVGSSGGSFATTPGQPARTESPAATNASE
                     PQSTEPGLDLEPAKLRCLPRIYTFKRGLFAGALSAVGLVGLASAIALSAKDGDVYLPA
                     DGDLYPTDMLNNFGRTARSLYGVAALGIVGGAAALAPWERWTTSGVPACPETAQDRWT
                     FRRGLAVGAFGSLTVAGLVVSGAMAGLDGKTWGFNQIGTPVPYQMHTATQAGFGTTAG
                     MAIGLALSLLIP"
     gene            2786..3886
                     /locus_tag="PKL17_18770"
     CDS             2786..3886
                     /locus_tag="PKL17_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46835.1"
                     /translation="MFQRLWDDSYRRFRRRLFGLGAAVLLECVGLGCGPSFPPYWQLS
                     SEPSDASGNLDPNGQLRILALTAEPPEVAPGQSVRIAALVATHPRYGEIGSDGGVPQA
                     TLRPRGLSVLYRFCVLDAQLASPLPCGLFPQTQDFVPLPNRTDVPTVLDIPFAQFPVP
                     LAFGNPYQIVVTLIAADSSFDGGANGCAESAAQNGGVSPIPNHCVIAIKRVKVSRSDS
                     PNHNPKLRAVSLGSDDASLVDLATGNATYPKLGSDVADSDRPFFHLVVERSADSEEQE
                     PDPKDQSQTRAELLTTSLFVTSGNVDSGRGNFIDLDCKTDCPQRLQTTYLWQPPALRA
                     MQESPDDRTHFVVVLRDDRGGSDFAIGTAKAR"
     gene            4104..5819
                     /locus_tag="PKL17_18775"
     CDS             4104..5819
                     /locus_tag="PKL17_18775"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNK46836.1"
                     /translation="MTAANRLPNSMVMSGQTNPVANQLAAQTDDQSGARAVEITALFD
                     GAVQDVCHLANPHAGTISAFTKGVLALSAVSLGTVVALFGSAYAEVRAEKATEELSQK
                     KTPTKNHSKERARDAAAGGLLFLGTAGLLYGIQRLGSERGEREYSVGIDPRASYKVST
                     EGLPSVRFPLVRSTGNAFELVVADAMKGTVTRDGKQMDLHEYAKSERSARDGQLADSS
                     VLAIPDGVRFSIEHGGATFLVQSVAKPRHYPVPLRIDWATQSYTGAVMAGAAAFVGMM
                     FALPPDARSLSLDGFDMVRLVKLNAKPEAEEDPAWLKPQKQEKQTTEKAASPSKDNHA
                     KVRTTTPVPQTYVVKNPGPRSREEKQETARNAAANAGIFVAMRGSMAMTVFSRESVFG
                     SDAKEAMDNVVGAEGVDGYSSGGNIVGTGPIGGGGGDGTIGTGLPFGPGGPGGRRGPA
                     GPGLKRYIAHAPVTEVGKVEVSAGTMDREVIRRVIRSHMKEVKYCYEKELMAHKELEG
                     RVQVKFLIGPMGKVATSLIESSSLRNAGTEQCVAEAVRRWEFPKPPSGPVTVTYPFVF
                     KPAGE"
     gene            5920..7302
                     /locus_tag="PKL17_18780"
     CDS             5920..7302
                     /locus_tag="PKL17_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46837.1"
                     /translation="MRWAGRIPSEVIAACRRLQETGFAAYVVGGAVRDLLLSPDDDKA
                     KDFDLTTSATPDEVIAVFGRRKVIPTGIAHGTVTVLCERAAGKPHPVEITTFRGETGF
                     SDGRRPDRVEFITDLTEDLRRRDFTVNAIAFDPVSQRLVDPFDGQGDLGSQTIRAVGD
                     PVERFAEDGLRVMRAVRFSAQLGFEVDVYTRAAFAGALPTLRKVSRERIRDELLKLLA
                     SPAPRRGLRLMCLRSDANPSFDWGTDGNILRVVFPELSHMVTDLSSLASWCNLIEQVQ
                     RPLRLAAFLWPLREWLQGLGAQVAKLPRALGELLDERLKLPASDRQFLVSILTVPLVP
                     SELALHLSGPQLRRFLAEHPEPVIDALLALQWAAGKAARDEAASVQLPLLMDRIAEER
                     ARKPPLSIAELAVGGKDLIAACQLPPGPWVGELQRELLLQVLDEPSRNTREDLLAIAT
                     KWLTTRNFRH"
     gene            7345..7428
                     /locus_tag="PKL17_18785"
     tRNA            7345..7428
                     /locus_tag="PKL17_18785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7379..7381,aa:Leu,seq:cag)
BASE COUNT         1455 a         2170 c         2339 g         1465 t
ORIGIN      
        1 ggagctggtt tgatactcgt cactcgttcc gcgcggcgac acataaagtg ttccgctgat
       61 gttttcgagc acgacgcgat acggtggtgc ggggcgacga accaggcgca gacaggctcg
      121 cagcttgctt tcttgtaccg cggtgagctt gccgtcttgg ctgcttacat cgacttgcac
      181 actggccgcg ggatcgggcg tgtccactgt ggggggcggc ggcagctcgt cgcctgcgcc
      241 gattccgcca tcgcgacgga tttgttgcgg tcgcggtccc tgcggtttgg gaatctgcgg
      301 acttgtcggc ttggtggttg tcgcagtcgc tgttttggcg gcaatcggtg tatcgcttgc
      361 gctgcgcagc ggcggcagct tggccagcag cacttccatt cccgcatccg gcgcgagcgg
      421 cgagcctgcg tccggcgttt cggtttcaat cgacggcgcg gcgacgatct gcggtggctg
      481 cgtccgcggc tttttgggaa acaaaagctt gcgcgcaccg agcaaaagtc ccagaccaca
      541 caccgcccac agcgcgcctt gcaccatcgg cagccacagc cttggccatg tggattccgg
      601 tctacggggc agggcggcga cccggccttc cgtcggtggt gaaaagcgcg aaaccgaacc
      661 accaagcgct gccacaaagt cgcgcagatg ggcaaagcgc acaccacgcg accgactgag
      721 cgctcgctga agtgcgagct cgacccgctt gctgagcaaa agtggcagcg gatcttcggc
      781 gagcactcgc tgcaccacga cctcgcgcgg ttcatcgggc gcaccgacga acgccggctg
      841 tccagcgagc agctcgtaca aaatcaccgc caagctgaac tgatcactgc gtggatcgca
      901 cgcttggccg ctcagcactt caggggccaa atagcctggt tcagcaaaac gggcggccag
      961 cgtttttgga tacagtccaa catcggtcag cttcacccgc cagttgtcac cgtcgctgat
     1021 aacgacaatt cggcgcggcg acaaacccaa gtgcagcaga tcgagccgat gcaccgcatc
     1081 cagcgcttcg ccgatttggc tcgccagatc ctgcacttcg gtttcgcgca gcggtcgtcc
     1141 gagcgagcgc aaaagttcgg gcagcgtctt tccttccagc tcgacgggtt cacgcagcag
     1201 acaggtgcgg ccttcttcat caaacggaat cagcgcgtgg gtctgtgaca gcgacggatg
     1261 ccggaagctc gcgagtcgct ggacaccgga cttccaatca agcagctcgt cctgtggcag
     1321 ctcggggggt ccgcccggcc agatcagcag cttgagcagc agcggtcggc catcttcgac
     1381 ggcgcgcacg gcatagctct cctccgcacc gattcggtcg aggaaacgct cgacacgata
     1441 gcgcgcggag acgagatcac cgggccgaag cgccggggtc gcgctgtctg cttgggacat
     1501 cttgaaagcc tacggaatca gcagcgacag tgccaggccg atggccatac cggcggtcgt
     1561 gccgaagcca gcctgggtgg cggtgtgcat ttggtacgga accggagtgc cgatttggtt
     1621 aaatccccag gtttttccgt cgagaccagc catcgctcca gacaccacaa gaccagccac
     1681 tgtcaagctg ccaaacgcac cgacggcaag tccgcgacgg aaggtccagc gatcttgggc
     1741 ggtttccggg caagccggca cgccacttgt tgtccagcgt tcccacgggg ccagggccgc
     1801 cgctccaccg acgatgccaa gcgccgccac gccatacagc gagcgtgcgg tgcgaccaaa
     1861 gttgttgagc atatcggttg ggtacaggtc tccgtcggca ggcagataga catcgccgtc
     1921 tttggccgac agtgcgatcg cgctggccaa gccgaccaga cccaccgccg acagtgcacc
     1981 cgcaaacaga ccgcgcttaa acgtgtagat ccggggtaga cagcgcagct tggccggctc
     2041 caggtcgagc cctggttcgg tgctctgcgg ttcgctggcg tttgttgcgg cgggcgattc
     2101 agttcgcgcc ggttggcctg gtgtcgtcgc aaaagagcca cccgagcttc ccaccggcgg
     2161 aggcgccacc ggcaccggca caagctgggg cgacggagca gagggcgccg gggcgacagg
     2221 aaccagcgct gcctgggctg cgagcgcatc gaccagtcga cctgcggcgc gcgaaactga
     2281 ctcttgcagc agttcttccg aacagtcggt gcagcgttcc tcaacgagtg tcggctgttc
     2341 cgcatccaga tcgtaaagcc acagtcgcac cagatagctg cgatcgtttg ggaagacttc
     2401 accaccgatg agccgccgcg cggaaaacgc cttgcccagt cgggtcaggc agggtttttc
     2461 ggtacacagc agctccgacg actggagact gggcgacacc gccggttccc ccatccgcaa
     2521 aaggtgctgc gccaccaccg agcgcgattt tgcggaagac tgaccttcgc gctttatccc
     2581 gagcaccaaa atcagcggtg cggcgtgcgc aggcagcgac gaaaagccaa tcagcgcggc
     2641 caaaagccac cacatgccag ggcggaaaaa acgcagcgaa ccggattctc gactcgatcc
     2701 cagagcgcga aaattgggca acattttgcc taaaagcaga ccagcactcc ctatacgctg
     2761 tcaatcggca aaacacgagg ccacgatgtt ccagcgactt tgggatgaca gttacagacg
     2821 cttccgtcgt cggcttttcg gacttggcgc ggctgtactc cttgaatgcg tgggacttgg
     2881 ctgtggacca agctttccgc cctattggca gttgtcatcg gagccgtctg acgcgagcgg
     2941 caaccttgat cccaacgggc agctgcgcat cttggcgctc acggcagaac ctcccgaggt
     3001 cgcgccgggg cagtcggtcc gcattgcggc gctcgttgcc acgcatcctc gctatggaga
     3061 aatcgggtca gatggtggcg ttccgcaggc gaccttgcga ccgcgtgggc tgtcggtgct
     3121 gtatcgcttc tgtgtgctgg acgcgcagct tgcttcgcct ttgccatgcg gtctgtttcc
     3181 gcagacacag gactttgtgc cgctgccaaa tcgaacggat gttccgacgg tgcttgacat
     3241 tccgtttgcc cagtttccgg ttccgctcgc gtttgggaat ccgtaccaaa tcgtcgtgac
     3301 gttgatcgct gcggattctt cttttgatgg tggcgccaat ggctgtgcag agtcggctgc
     3361 gcaaaacggt ggtgtgtcgc cgattcccaa tcactgtgtc atcgccataa agagagtcaa
     3421 agtcagtcga tcagacagtc ccaatcacaa tcccaagctg cgcgccgttt ctctgggcag
     3481 cgacgatgca agcttggtcg atctggcgac cggaaacgcg acttatccca agctgggaag
     3541 tgatgtggcc gattctgatc gaccgttttt ccatctggtt gtcgaacgga gtgcagattc
     3601 ggaagagcag gaacccgatc caaaagatca gagccaaacg cgcgccgaac ttctaacaac
     3661 atctctgttt gtgacaagtg ggaacgtgga ttcgggacgt gggaatttca tcgatctcga
     3721 ctgcaaaacc gactgtccgc agcgactaca aaccacctat ctgtggcagc caccggctct
     3781 gcgggcgatg caagaatcac ccgatgacag aacgcacttt gtcgtggtgt tgcgcgatga
     3841 tcgcggaggc agcgactttg cgattgggac tgccaaagcg cgctaaatcg acccattaag
     3901 agaccgtttc atcgcgcagg ttcccaaact tttatactaa agatttctcg tcgaaaagtg
     3961 gctgattttg ctgtcttttt ctgggctgtc gatcggcaaa tatggatgcc ttcctttttg
     4021 tgcgatcgct tggaacctcc gctctctcct tttgtctcta gttgcgaaga cggacgactt
     4081 aggaagattg aaggagaact cccatgacag ccgcaaatcg ccttcccaac agcatggtca
     4141 tgagcggaca gacgaacccg gttgcgaacc agcttgcagc ccagaccgat gatcaatccg
     4201 gcgctcgtgc cgtcgaaata acggctctgt ttgatggtgc agtacaggat gtctgccatc
     4261 ttgccaatcc acatgcagga acgatctcgg cgtttaccaa aggtgtcctg gctctgtctg
     4321 cggtatcgct gggaacagtg gtcgcgctgt ttggttcggc ctatgcagag gtgcgggcgg
     4381 agaaggccac agaggagctt tcgcagaaga aaactccgac gaaaaaccac agcaaagaac
     4441 gagcacggga tgcggcggcg ggcggactcc ttttcctggg aacggcggga cttctgtacg
     4501 gaattcagcg gctcggcagt gagcgcggcg agcgtgaata cagcgtagga attgatccgc
     4561 gcgcaagcta caaagtcagt acagaaggtc ttccaagtgt tcgctttccc ttggtgcgca
     4621 gcactggaaa tgcgttcgag ctggtcgttg cggatgcgat gaaaggaacc gtaacccgcg
     4681 acggaaagca gatggatctt catgagtatg cgaaaagtga gcgcagcgcc cgtgatggtc
     4741 agcttgcaga ttcatcggtc cttgcgattc ccgatggagt ccgtttttcg attgagcatg
     4801 gcggagcgac attccttgtg cagtcggtgg ccaagccgcg gcactatccg gttccgctgc
     4861 ggatcgactg ggcaacgcag tcctatacgg gtgcggtgat ggcgggagcg gcagcgtttg
     4921 tgggaatgat gtttgcgctg cctcccgacg cgcgctcgct gtctttggat ggctttgaca
     4981 tggtgcggct cgtcaagcta aacgcaaagc cagaagcaga ggaagatccc gcgtggctga
     5041 agccacagaa gcaggaaaaa cagacgacgg aaaaagcggc gtctccgagc aaagacaacc
     5101 atgccaaggt gcgcaccacg actccggttc cacagacgta tgtggtgaag aatccgggtc
     5161 cgcgcagcag agaggaaaag caagaaacgg cgcgcaacgc agcggcaaac gcgggaatct
     5221 ttgttgcgat gcgtggttcg atggcgatga ctgtcttttc gcgcgaatcg gtgtttggga
     5281 gtgatgccaa agaagcgatg gacaacgtcg tcggggcgga gggagtcgac ggatacagca
     5341 gtggcggaaa catcgtcggg actggcccga tcggtggtgg ggggggagac ggaaccatcg
     5401 gaaccgggct tccctttggt ccgggggggc ctggcggtcg gcgcggtcca gcgggtccgg
     5461 gcctaaagcg ctacatcgcg cacgcacccg tcacggaagt cggcaaagtg gaagtcagcg
     5521 ccggaacgat ggatcgcgaa gtcatccgcc gcgtgattcg cagccacatg aaggaagtga
     5581 agtactgcta cgaaaaggaa ctgatggcgc acaaggaatt ggaaggccgc gtgcaagtga
     5641 agttcctgat cggtccgatg ggcaaagtgg cgacgagtct gattgagtct tcctctttgc
     5701 gcaacgccgg tacagagcag tgtgtggcgg aagcggtgcg ccgctgggag ttcccgaagc
     5761 cgccgagcgg acccgtcact gtgacctatc cgttcgtgtt caagcccgcc ggagaatagc
     5821 ttccgcttct tcaccttcgc ggttctttcc ttcttcatcc acatccacgg cgaatttttc
     5881 ttcacctacg ccgcttgctt ggaccaccat gcgaccacca tgcgctgggc cggtcgcatt
     5941 ccctctgaag tcatcgctgc ttgtcgccgc ttacaagaaa caggtttcgc cgcttatgtc
     6001 gtcggtgggg ccgttcgcga tctgctgctg tcgccagacg atgacaaagc caaagacttt
     6061 gatctcacca cgtccgcgac gcccgatgaa gtgatcgcgg tgttcggtcg ccgcaaggtc
     6121 attccgaccg ggatcgcgca tggaacggtc acagtgctgt gtgagcgggc cgcaggaaag
     6181 ccacatcccg tcgagatcac gacgtttcgc ggcgaaaccg gctttagcga tggccgtcgt
     6241 cccgatcgcg tcgagttcat caccgatcta accgaagacc tgcgccgccg cgacttcacg
     6301 gtcaatgcga tcgcgttcga tccggtgtcg caaagactgg tcgatccgtt tgatgggcag
     6361 ggtgatttgg gtagccagac gattcgtgcc gtcggtgatc cagtggaacg ctttgccgag
     6421 gacggcctgc gggtgatgcg cgcggtgcgc ttttccgccc agcttggctt tgaagtggat
     6481 gtgtacacgc gcgcggcttt tgcgggggca ctgccgacgc tgcgcaaagt cagtcgcgaa
     6541 cggattcggg atgaactgct gaagctgctg gcttcgcccg cgccgcgtcg cggtcttcgg
     6601 cttatgtgcc tgcgcagcga cgcaaatcca tcgtttgatt gggggacgga cggcaacatc
     6661 ctgcgcgtcg tgttccccga gctttcgcac atggtgacgg atttgtcgtc tctggcgtcg
     6721 tggtgcaatc tgatcgaaca ggtgcagagg ccgctgcgtc tggcggcgtt tctgtggccg
     6781 ctgcgcgagt ggctgcaagg ccttggggcg caagtggcga agctgccgcg cgcgcttggt
     6841 gagcttctcg acgaacggct caagctgccg gcgagcgatc ggcagttttt ggtgtcgatt
     6901 ctgacggtgc cacttgtgcc aagcgagctt gcattgcatc tgtccggccc gcagctccgt
     6961 cgttttttgg ctgaacatcc cgaaccggtg atcgatgcgc tgcttgccct tcagtgggcg
     7021 gcggggaaag cggcgcgcga cgaagccgcg agcgtgcagc tgccgcttct gatggaccgc
     7081 atcgccgagg aacgtgcccg caagccgccg ctgtcgattg ctgagctggc ggtgggcggc
     7141 aaagacctga tcgcggcgtg ccagcttccg cctgggccct gggttggcga gcttcagcgg
     7201 gagctgcttt tgcaagttct cgacgagcca agccgaaaca cgcgcgagga tctgctggcg
     7261 atcgccacaa agtggctaac tacgcgaaat tttaggcatt gacagcggtg gggaaggcgc
     7321 gctagaaacg tcctcacctc gctcgcgaaa gtggcggaat tggcagacgc gcaggattca
     7381 ggttcctgtg aggtaacact cgtgagggtt caactccctc ctttcgcac
//
LOCUS       DAORGN010000230         7396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4613_length_7396_cov_5.037870, whole genome shotgun sequence.
ACCESSION   DAORGN010000230 DAORGN010000000
VERSION     DAORGN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_7396_cov_5.037870"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PKL17_18790"
     CDS             <1..1180
                     /locus_tag="PKL17_18790"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNK46838.1"
                     /translation="GVAVSQSLLKLLGKQRKQVLGLMSGTSGDGLDCALVDLSGHGLS
                     VEIHGVRSLTFPYQPAEKSFIHSLFEPDVDSRTLCQASFRLGDRFAQGVIDFCAELGI
                     SPRDVDLIGSHGQTVWHQPPSACLRGEVPSTLQIGEPAVIAAKTGVVTIGDFRVADVA
                     LGGEGAPLVPYFDYLLFRQPGQRRALQNIGGIANVSVLGDSPDSVIAFDNGPGNMLID
                     ALAQVVSHGMLDIDQDGGLSARGQVLPTVLSTLLDDDYLRRKPPKSTGRERYGVGFAE
                     ALLRRHADREPVDLLATVVAFTAHAIADSLRDFRLDELLVSGGGAYNRTLMGQLERLI
                     APTKLSKLDAQGIDIDNKEAVAFAILALQTLHGEPANLPSVTGARGQAVLGKICLPPL
                     "
     gene            complement(1187..2722)
                     /locus_tag="PKL17_18795"
     CDS             complement(1187..2722)
                     /locus_tag="PKL17_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46839.1"
                     /translation="MKYLVAALASIGLFLPSARADRIDFLGELRSSSMVGFANAGQFG
                     TGDDRGPCGVSPRFDFGQEVSGTATMLATSRRYRHQLSLSVGYIAQYCTPILRRPVFG
                     LDYKAEHALTSRTRLSGQARATVDVFDRTLDVRSGSGAMVDPNLPTTTQSASGQFFIT
                     PSASIELSHAFSEQYGLRVGLSWKALELLEAVDTLPTYSTLGPMESVEVSVLALRNFD
                     RHRFDLPLRYRLSALYSGTLRDILPRSDVKPAHDVFVGAAWEYKLDQRFTVRTETGLI
                     AAAQPHLCTRLDPLLVAGNRCSIDGRVQGIRGNENPPPLEVAIGTVETLSFGGEVSLG
                     YVGPRRRFDVRLARGYEPDPYAGALSLWDRLGGDFLIRPFWELAIYGSAQLMHGTQTS
                     PGRVSPPSEDPFLQLVSPQNRTMYLLISSVGGSYRVVGPLSVFAEGNVFAMAIRGERV
                     PEYPALGQPVKAEVSRFPTDPTQSTQNSSRFALQLGIRVQFDTLPVPRRESELLRDAR
                     ALP"
     gene            complement(2719..3861)
                     /locus_tag="PKL17_18800"
     CDS             complement(2719..3861)
                     /locus_tag="PKL17_18800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK46840.1"
                     /translation="MHILHVIQSASQIYGAERCVLDETLALVQRGHQVSVLLCHETRL
                     GDDQDRLQHELTTRGIPTLRVEAASQLSPQLVLAWLRALRRIHPDVVHSHSLKTDVLS
                     APLCRLIGLPLVIEVHGYLRPDDLRVRFYEKLDRLSLRLASAVLTLSQEYRAEVIASG
                     VSPERTYLLPSGIDLDRLRAQIGERDLRSELRKTSRSDDEVVLGMVARLSPEKGHEQF
                     LTAFAKLSSLGLPVRGVLYGEGPLAESLRRRIVDEKLNITMLGYAPQIADAYRSCDVL
                     VSCSHNEGLPLNLIEAMALGVPTVAMATGGCAEIVEDGQTGLLVARGDVAALTQALLQ
                     LVADPSLRQRMGQAAMQSADARFSLSTWAARVEAVYQKAQPKTGRG"
     gene            complement(3889..6210)
                     /locus_tag="PKL17_18805"
     CDS             complement(3889..6210)
                     /locus_tag="PKL17_18805"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNK46841.1"
                     /translation="MRTPPEQLSLTLHAAPLLLVAVLWGGTTARPATMVVAGLCGLLS
                     MWRGLALRTVPVSLYSLWLLLAAGIAAIQCVPLPAALRSMLPLASEPLLRQTLADIGG
                     YPHKPLPLSLDPAETANEAVRLLGYVCALGVFSTLRTRHGDSYRLAGMVVMVGSLVAG
                     LGSLAALGVTLPGTLAVTTEGQSRALWPAVLQNANHMAALLSLTAILALGMLLQTDRQ
                     ARPQRFGALCAVLGLVNLALLFSLSRAGILCGLFGQLLTLLSADSPVPKTKRQRLWWV
                     VLPSLGLLLAMLFGPAAPLVGRWRELSHGLSPAGSKVWLWQTAWPLLKAHPLTGIGRG
                     ALETALQAPTAIATTTRFTHLENEWLQALLDHGLPLGFVLWGLLFCATAQAARQLFRS
                     SGESPTPVRRAALWALLCLGLHNLFDFNLQTGAVALSALLVTSLVQKPLFSLSARWLA
                     GLGALVLLLAAVVQLKLPSHDEDGRRLAILAQDPTVSPSVLKQAASEALFRHPLDSYL
                     SAVVAARLFEDDDPEAMAWTNRSLRQNPADLLARATAVRLLGRHGHQRQALAMLGPLI
                     GEADAEKRRYLFGLLLSLSPKGTELVAALPNDEPLRLALLEFLGTQSQPNWPLLLSVA
                     SESVSRGDQAALTWLGRAALSTAQPDAAETALRGLLTRPQLEPLLIGGLLEVLLRGQK
                     LVLAKDLAEVALRRQKAPEISIAHAQILAMAGDLDGARQSLAQAVMQTSDRLLLARLH
                     EVRADLEQRQGHVHRAETERAEAQRLRRDAGQP"
     gene            complement(6194..>7396)
                     /locus_tag="PKL17_18810"
     CDS             complement(6194..>7396)
                     /locus_tag="PKL17_18810"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNK46842.1"
                     /translation="ARELYFQRSLKLGVLRLLQMFSREQPSVDERAQLAEFCHRALEK
                     IAAPIQRIGFDFVAISSGTGRALAEICTPLVPRSDAPSSKDKAERSKVVRFSDIFALE
                     QRLFAMTPAERQKMPGLDPRRADSILPGVVLVRSLLEVFHADEYVLCEAGLREGLIFD
                     YAQKHRPDIQLSDEFPDLRRRSVVSLMRRCQVREPHAKQVARLALDIFRGLRPLHGLA
                     NYDGELLEFAALLHDAGFYIAASKHHKHGQYLIENVGLSGFSSEEIKILSHCVRYHRK
                     STPKDTHESFVALGEPLRRKVRLLSSILRVADGLDRTNRQVIESVRCRTQSKEIELTI
                     AVPEGTDLELELWSTRRKADLIEEVFRRKLRFGIVPTQKGTSEEDATTAREDFWQTAQ
                     AHPRENAS"
BASE COUNT         1443 a         2304 c         2340 g         1309 t
ORIGIN      
        1 aggtgtcgcg gtgagccagt cgctgctgaa gctgctcggg aagcagcgca agcaggtact
       61 cggtctgatg tcaggaacat caggtgacgg gctcgattgc gcgcttgtcg atctttccgg
      121 ccatggactt tccgtcgaaa ttcatggcgt tcgttctctg actttcccct atcagccggc
      181 tgaaaagtcc ttcattcact cgctgttcga gcccgatgtc gattcgcgca cgctctgtca
      241 ggcgtcgttt cggctggggg atcgttttgc gcagggcgtg atcgattttt gtgccgagct
      301 ggggatttcc ccgcgtgatg tggacctcat cggcagtcac ggacagacag tgtggcatca
      361 gccgccgtcg gcgtgtctgc gcggcgaagt tccgtcaacg cttcaaatcg gcgagccggc
      421 ggtgattgcg gccaaaaccg gcgtggtgac aatcggggac tttcgcgtcg ctgatgtcgc
      481 gctgggcggg gaaggggcgc cgcttgtgcc gtattttgat tacttgctgt ttcgacagcc
      541 agggcagagg cgggcgctgc aaaacatcgg cggaattgcc aacgtcagcg tgctgggcga
      601 ttcacccgat tccgtgatcg cgttcgacaa tgggccgggc aacatgctga tcgatgcgct
      661 ggcgcaggtg gtgtcgcacg gaatgctcga catcgatcaa gacggagggc tgtccgcgcg
      721 aggtcaggtg cttccgacgg tgctttcgac actgctcgac gatgattatc tgcggcgaaa
      781 accacccaag tcgacgggac gggaacgcta tggggtcggc tttgcggaag cgctgctccg
      841 tcggcatgcc gatcgtgaac ctgtcgattt gctggcgacg gtggtggcgt ttaccgccca
      901 tgcgatagcc gacagcctgc gcgattttcg gctcgacgag ctgcttgtgt ctggcggtgg
      961 ggcgtataac cgcacgctga tggggcagct cgagcggctg attgcaccaa cgaagctgtc
     1021 caagctcgac gcgcaaggca tcgacatcga caacaaagaa gcggtcgcgt ttgcgatcct
     1081 ggcgctgcag accttgcacg gtgaaccggc gaacctgccc agcgttacgg gcgcgcgcgg
     1141 ccaagcggtg ctcggcaaga tttgtctgcc gccgctgtag ctgcggtcag ggcagggcgc
     1201 gggcgtcgcg cagcagctcg ctttctcgtc tggggaccgg cagcgtatca aactgcaccc
     1261 gaatgccgag ctgcagcgca aagcgcgacg agttttgcgt gctctgcgtc ggatcggtcg
     1321 gaaagcgcga aacttcggct ttgaccggct gcccgagcgc cggatattcg gggacacgct
     1381 cgccgcgaat cgccatcgca aagacattcc cttcggcaaa caccgacagg ggtccgacga
     1441 cgcgatagct tccaccgacg ctgcttatca gcaaatacat ggtgcggttc tgcggcgaga
     1501 caagctgcaa aaagggatct tcagacggcg gacttacgcg gcctgggctg gtctgcgtcc
     1561 cgtgcataag ctgcgccgat ccatagatcg ccaattccca aaatggtcga atcagaaagt
     1621 cgccgccgag ccgatcccag agcgacagtg caccggcgta gggatcgggt tcgtagccgc
     1681 gcgccaatcg cacgtcgaag cgccgtcgcg gaccgacgta acccaagctg acttcgccgc
     1741 caaaactgag cgtttcgacg gttccgatcg ccacttcgag cggcggcgga ttttcgttgc
     1801 cgcgaatgcc ttgcacccga ccgtcgatcg aacagcgatt gccagcgacg agcagtggat
     1861 caagccgggt acacaggtga ggctgcgccg cggcgataag tccggtttcg gtacggacgg
     1921 tgaatcgctg gtccagcttg tattcccaag cggcgccgac aaacacgtcg tgggcgggct
     1981 ttacgtcgct gcggggcaaa atgtcgcgca aggtgccgga atacagcgcg ctcagacggt
     2041 aacgcagcgg cagatcgaag cgatggcgat cgaagttgcg cagcgcgagc accgatactt
     2101 cgacggattc catcggcccc agcgtcgaat acgttggcag cgtgtcgacc gcttccagaa
     2161 gctccagcgc tttccacgac agaccgacgc gcaggccgta ctgttcggaa aaagcgtggg
     2221 acagctcgat gctggcgctc ggggtgatga aaaactgacc cgatgcggac tgggtggtcg
     2281 tcggcaagtt ggggtccacc atcgcgcccg atccgctgcg gacatcgagc gtgcgatcga
     2341 acacatcgac ggtggcccgc gcttgtccgg acaggcgggt tcggctcgtc agcgcgtgct
     2401 cggctttgta atcgagacca aacacggggc gacggaggat cggggtacag tactgcgcaa
     2461 tgtagcccac cgacagcgag agctggtggc gatagcgacg gctggtcgca agcatcgtcg
     2521 cggtgccgct gacttcttgg ccgaagtcaa agcgcgggct tacaccacac gggccgcggt
     2581 cgtcgccagt accgaactgg ccggcattgg cgaaaccgac catcgacgat gagcgaagct
     2641 cgccgagaaa gtcgatgcga tcggcgcgcg cgcttggcaa aaaaagcccg atcgaagcga
     2701 gtgccgcgac gaggtatttc atcctcgacc tgtctttggc tgggcttttt gataaaccgc
     2761 ctcgacccgc gccgcccagg tcgaaagaga aaaccgagca tcggcagact gcatcgcggc
     2821 ctggcccatc cgctgccgca gcgacgggtc ggccaccagc tgcaaaagcg cctgcgtcag
     2881 cgcagcgacg tccccgcgcg ccaccagcag accagtttgg ccatcctcga cgatttcggc
     2941 gcagccgccg gtcgccatcg ccaccgtcgg aactccgagt gccatcgctt caatcaagtt
     3001 gagcggcaga ccttcgttgt gtgaacacga caccagcaca tcgcatgagc gataggcatc
     3061 ggcgatttgg ggcgcatacc caagcatcgt gatgttgagt ttttcgtcga caatccgtct
     3121 gcgcagcgat tccgcaagcg gtccttcgcc atacaggacg ccgcgcaccg gcaggcccag
     3181 cgacgaaagc ttggcaaacg cggtcaagaa ctgttcgtgg cctttttccg gcgacagccg
     3241 cgccaccatg ccgagcacca cttcatcgtc ggaccggctg gttttgcgca gctcgcttcg
     3301 cagatcgcgc tcgccgattt gtgcgcgcag ccggtcgagg tcgatgccgc tcggcagcag
     3361 atacgtccgt tcgggcgaca ccccgcttgc gatgacttcg gcgcggtatt cctggctcag
     3421 cgtcagaacc gccgacgcca gccgcagcga cagccgatcg agcttttcgt agaagcggac
     3481 ccgcagatca tcgggtcgca ggtagccgtg gacttcgatg accagcggca ggccaatcag
     3541 ccgacacagt ggcgccgaca gcacatcggt cttgagcgag tgcgagtgca ccacgtcggg
     3601 atgtatcctg cgcagcgcgc gaagccacgc gaggacaagc tgcggactca gctgggatgc
     3661 cgcctcgaca cgcagcgtcg gaatccctcg cgtcgtcagc tcgtgctgta gtctgtcctg
     3721 atcgtcgccg agccgggttt catggcacag caggaccgaa acctgatggc cacgctgcac
     3781 aagcgcgagt gtttcgtcga gaacgcagcg ctctgcccca taaatttggc tggcagactg
     3841 gatgacgtga aggatgtgca tcgggcgacc gaatctgctc gcaatctatc acggctgtcc
     3901 ggcgtctcgg cggagccgct gggcttctgc gcgttcggtt tctgcgcggt gaacgtggcc
     3961 ctggcgctgt tccagatcgg cgcgaacttc gtggagccgg gccaaaagca gtcggtcact
     4021 ggtctgcatg accgcctgcg ccaaggactg tcgggcaccg tcgaggtcgc cagccatcgc
     4081 cagaatctgc gcgtgggcga tgctgatttc gggggctttt tgtcgacgca gcgcgacttc
     4141 ggccaggtct ttcgcgagca cgagcttttg tccgcgcagc agcacttcca agagtccacc
     4201 gataagcaaa ggctcaagct gcggccttgt gagcagtccg cgcagtgccg tttcggcagc
     4261 atcgggctgt gcggtcgaaa gcgcagcccg tccaagccat gtcagcgctg cctgatcgcc
     4321 acgagacaca ctttcggacg ccaccgacag aagaagcggc caattgggct ggctttgggt
     4381 gcccagaaat tccagcagcg ccaagcggag cggttcgtca tttggcagcg ccgccacaag
     4441 ctctgttcct tttggggaca gtgaaagcag aagaccaaac aaatagcggc gtttttccgc
     4501 gtcggcttcg ccaatcaggg gccccagcat cgccagcgct tgccgttggt gcccgtggcg
     4561 accaagcagc ctgaccgcgg tcgcgcgggc cagcagatcg gcgggatttt gtcgcaggct
     4621 gcggtttgtc cacgccatcg cttcggggtc atcgtcttcg aaaagccgcg ccgcaaccac
     4681 cgccgaaaga taggaatcga gaggatgccg aaacagcgct tcgctcgcag cctgcttgag
     4741 cacggagggc gaaaccgtcg ggtcttgcgc caagatcgct agccgtcgtc cgtcttcgtc
     4801 gtggctcggc agcttgagct gcaccaccgc ggccaacagc agcaccagcg cccccagacc
     4861 ggccagccag cgtgccgaca gcgaaaagag aggcttttgc acaagcgacg tcaccaaaag
     4921 tgccgacagc gccaccgctc ccgtctgtag gttgaagtca aacaggttgt gcagtcccaa
     4981 acagagcagc gcccaaagtg ccgcccgtcg aaccggcgtc ggcgattccc ctgagctgcg
     5041 aaacagctgc cgggccgctt gcgccgtcgc acaaaacaaa agtccccaca gcacaaaccc
     5101 gagcggcagc ccgtggtcga gcagcgcctg cagccactcg ttttccagat gggtaaagcg
     5161 ggtcgtcgtc gcaatcgctg tgggggcttg cagcgcagtt tccagcgcgc ctcgtccgat
     5221 gccggtcagc ggatgcgctt tcaaaagcgg ccacgccgtc tgccacagcc agactttgct
     5281 gcccgccggc gacagtccgt gtgaaagctc gcgccagcgt ccgacgagcg gggctgccgg
     5341 tccaaacagc atcgccaaaa gcagacccag cgacggcaaa accacccacc agagccgctg
     5401 tctttttgtc ttgggcacag gtgaatcggc agacagcagc gtcagaagct ggccaaaaag
     5461 accacacaaa atgccggctc gcgacagcga aaacagcagc gccaggttca caagcccaag
     5521 cacggcgcac agtgccccaa agcgctgcgg acgcgcctgc cgatccgtct gaagcagcat
     5581 ccccagcgcc aaaatggccg tcagcgaaag cagcgccgcc atgtgattgg cgttttgcag
     5641 caccgccggc cacagcgcgc ggctctggcc ttccgtcgtc accgcgagtg tcccaggcag
     5701 cgtcacgccc agcgccgcca acgatccaag acccgcgacg agcgatccga ccatcaccac
     5761 catcccggcc aagcgatagc tgtcgccgtg acgggtgcgc agcgtcgaaa aaacgcccag
     5821 cgcgcagaca tagcctagca atcgcacggc ttcattggcc gtttcagccg gatcgagcga
     5881 aagcggcagc ggcttgtgcg gatatccacc gatgtccgcc agcgtctggc gcaaaagcgg
     5941 ctcgctcgcg agtggcagca tgctgcgcag cgctgcggga agtggcacgc actggatcgc
     6001 tgcaattccg gcagccaaca agagccacag cgaatacagc gacaccggca cggtgcgcag
     6061 cgcaagccct cgccacatcg aaagaagtcc acacagaccc gccaccacca tcgtcgctgg
     6121 gcgtgcagtt gtgccacccc acagcaccgc gacaagcaaa agcggcgcag catgcaaagt
     6181 cagcgatagc tgttcaggag gcgttctcac gagggtgcgc ctgcgcagtc tgccagaagt
     6241 cttcgcgggc ggtggttgcg tcttcttcgc ttgtgccttt ttgcgtcggc acgatgccaa
     6301 agcgcagctt gcggcggaac acttcttcga tcaggtcggc tttgcggcgc gtcgaccaca
     6361 gctctagttc gaggtcggtg ccctcaggga cggcgatcgt cagttcgatt tctttgcttt
     6421 gggtgcggca gcggaccgat tcaatcacct ggcgattggt tcgatcgagc ccgtcggcaa
     6481 cgcgcaaaat cgacgacaaa agacggactt tgcggcgcag cggctcaccg agcgcgacaa
     6541 agctttcgtg ggtgtctttc ggcgtcgact tgcggtggta gcgcacgcag tggctgagga
     6601 tcttgatctc ctcgcttgaa aaacccgaca ggccgacgtt ttcgatcagg tactggccgt
     6661 gcttgtggtg tttcgacgct gcgatgtaaa agccggcgtc gtgcaaaagc gcggcaaatt
     6721 ccagcagctc tccgtcgtaa ttggccaggc cgtgcagcgg tcgcagccca cgaaagatgt
     6781 cgagcgcgag cctggccacc tgcttggcgt gcggttcgcg aacctggcag cgacgcataa
     6841 ggcttaccac cgagcggcga cgcagatcgg gaaactcgtc ggacagttga atgtcagggc
     6901 ggtgtttttg ggcgtagtcg aagatcagcc cttcgcgcag accggcttca cacagcacgt
     6961 attcgtccgc gtggaacact tcgagcagcg accgcaccag cacgacgccg ggcagaatcg
     7021 aatcggcgcg gcgggggtcg aggccaggca ttttttgccg ttccgccggc gtcatcgcaa
     7081 acagccgctg ctccagcgcg aagatgtcgg aaaagcgcac gactttgctt cgctcggctt
     7141 tgtctttgct cgatggcgcg tcgctgcgtg gcacaagcgg cgtacagatt tcggccagcg
     7201 ctcgcccggt tcccgacgaa atcgcgacga aatcaaagcc gatgcgctga atcggtgcgg
     7261 caatcttttc gagcgcacgg tggcaaaact cggcaagctg ggcgcgttca tcgacgctgg
     7321 gctgttcgcg cgaaaacatc tggagcagcc gcagcacacc gagcttgagg ctgcgctgaa
     7381 agtacagttc gcgggc
//
LOCUS       DAORGN010000231         7385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4621_length_7385_cov_5.555116, whole genome shotgun sequence.
ACCESSION   DAORGN010000231 DAORGN010000000
VERSION     DAORGN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_7385_cov_5.555116"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="PKL17_18815"
     CDS             complement(<1..1352)
                     /locus_tag="PKL17_18815"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNK46843.1"
                     /translation="MQARIRLLSRSAEVRQIALGQLDEQDAADVYSARRALLEDSDGR
                     VRALAARFLGRAQAVSPVPWLHDATYDDLPSVREAAARALGQLGGLPAQRTLPRLTRL
                     ALLDSVWWVRRTATVALARIGQASAIPTLKAVLGDPFWRVRHAGIEALALLWQALPDE
                     REQILQVSATMNEACQSAHLELQSRVEPQLAAYKAPAPPALDQSLANPDPAVTTARLR
                     VRPPSDVPPAELLPLLADPHTALRTLAIERLARQIKATELEEVLPHMVIPGLPHALES
                     ACELLDRTGRRGQALIGRILSDNESPVGAVKWACQSLMRTGGRRAPLRATLLHPHPLA
                     RLAALRALDELGETDVTLFERLVEDEDAEVRSAALQALVVRVPDADLGRYLPELLGPD
                     SRPLCLLSLLGRAALSKDRVLLQRLADDEHPLLRARALRALGQMGRLGDATGHAHHPD
                     P"
     gene            complement(1484..2725)
                     /locus_tag="PKL17_18820"
     CDS             complement(1484..2725)
                     /locus_tag="PKL17_18820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK46844.1"
                     /translation="MGRPFPYRLFLFAFLGWTFDFYDLVLFAYIKDAVSHEFHLSHQV
                     ESWLLGVALSTSGLGGILAGYLADRYGKRSILALTVFVYSLGSAVCGLAPSLTVFLIG
                     RGLVGIGVGGEWAIGHGLVAEAVESRYRGRAAALLQAGEPLGAALAAVAGFLLLPLFG
                     WRAILLASSATALLAVAMRRSMVLPNERAHHHVPISEVFRSAGIGRRFVLAWVLAVLK
                     IGTYWTCYTWLPSFLLHEMGQRVGKSMLWVLTAQAGQFVGMLLFGNLADRAGRRPAFC
                     VYSLLTASALGLLAFNWKWLNQHPPLFWMTMLALGFGSGCTAGFGALLAELFPTEIRG
                     AAMGATYNLARSVQLLAPVVVAAMKVRYGLPGALTVPMVLALATASWVWMLPETRGIT
                     LPRMFMSPRTGSGSHPRRRPG"
     gene            2948..3871
                     /locus_tag="PKL17_18825"
     CDS             2948..3871
                     /locus_tag="PKL17_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNK46845.1"
                     /translation="MTKGGNLRLGVDLGGTKMEAVVLRLDGESFEVLARLRRPTDRAL
                     GYDHIIDALCGLIHDTAAQAGLAELPPIGIGMPGSISRRSGLTKNSNTTCLNGRPFRA
                     DVIAKLGQPVRFANDANCFALSEARFGAGRGHRVVFGVILGTGVGGGLVFPGADGRAD
                     AWDGPQGIAGEWGHVSLEPKTGPACYCGRRGCIETFLCGPAIESRYVERCGSPLSLPQ
                     IAERVAVDAVARQVCDAHVAMFGRAIATVINIVDPDVIVLGGGVSNLPWLYTDGVTEA
                     AKWVFSDELTTPIAKHQLGDSAGVIGAALLP"
     gene            3896..4519
                     /gene="trmB"
                     /locus_tag="PKL17_18830"
     CDS             3896..4519
                     /gene="trmB"
                     /locus_tag="PKL17_18830"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNK46846.1"
                     /translation="MPVRVRHHVNPFHLQFLDIAPDRLSAPPGRDPELQIELGCADAQ
                     FLFQLARRDPLGSYVGVEIRKAHVEDVNRRAATEGLPNLRAIFAHINLDLPTLFVGHR
                     IRRFFINFPDPWFKRAHHNRRIVNPDLAKTLTSLLVPGGELFFQSDIWELGISAMEVF
                     ETTPGLANVGGEWAFQKQNPYPAVSLREERVTEDGNPIWRILYRRVA"
     gene            4527..5183
                     /locus_tag="PKL17_18835"
     CDS             4527..5183
                     /locus_tag="PKL17_18835"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNK46847.1"
                     /translation="MKRAFLGLVLAASVVGCAGDLAGAQLVYQLKLQSSPVPATAMFV
                     EPQVWVEDPYSTKWKASSPIAAASLAGLTKRDQVSLLLRVSSADVASRVAPVTRFFDD
                     KQQLVAVGGGQLALFNDAVYSITLVEVSDARLLPLQVAKFAPASALAGRTVTMYGWGF
                     SPEAAISIGGQSVTDKQWLSPVEMVVRIPNAVAAGPLEVTVQNPDGTRDVRTDLLTAQ
                     "
     gene            complement(5224..7023)
                     /locus_tag="PKL17_18840"
     CDS             complement(5224..7023)
                     /locus_tag="PKL17_18840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK46848.1"
                     /translation="MSPPSDDATLTVSPNDPAQEAAHHAAPYLLVVEGNSSILCPLPP
                     SGELLIGRADDCGLRLSDRTASRHHARLRIDDDGITLIDLGSHNGTKVLGQRVVGSRM
                     LQHGDVIQLCEVTLVLHAPRLSSRPRELQPMSVLRQRLSEEVERSLRYGRPLALLCLQ
                     IDAGADVARLSLVLGQAARLLDVISREDQHVWLLLPELSADERTTTVAELMQSVLRVA
                     KTIRGGLASFPVDGGDPDSLLLSARTASSLAKPGECLSATEAHRTQPIGPHEVTIADP
                     AMQRIYALLERLAKSPIPVLIQGETGTGKEIAATALHVWSKRPGRFVALNCAALPETL
                     AESELFGHEKGAFTGAVSQKLGLLEQAAQGTLFLDEVGELPLSLQAKLLRALESQKIT
                     RVGGSRELPIDVRVVCATHRDLEHEVQQGRFRQDLFFRLVAARVMLPPLRDRRREIPL
                     LLRQFLRRAADHLGRSVPAFSDGALTLLLSHTWPGNIRELRNFCDYLAATSSDGIITS
                     TEVSSLLRQEPRDRNQASTPAVADQIGLSPNPTFRSLEDEIRELEQRRMMEALAKAQG
                     VQVRAAELLGMPIRTFSYKMKQYGISATGKSTK"
     gene            complement(7069..>7385)
                     /locus_tag="PKL17_18845"
     CDS             complement(7069..>7385)
                     /locus_tag="PKL17_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNK46849.1"
                     /translation="PDMTEEEQKNFPHKNVITRALGMRESVQVDIRHDTLLPGDTIFL
                     CCDGLTGMIDDQSILKIVLAAGEDLEKCVADLVDQANKNGGVDNITVIVMRAYKDDED
                     KK"
BASE COUNT         1449 a         2305 c         2245 g         1386 t
ORIGIN      
        1 ggatcgggat gatgggcgtg tcctgttgca tcgcccagac gtcccatctg gccaagggcg
       61 cgcagcgcac gggcgcgcaa cagcggatgt tcatcgtcgg caagtcgctg caaaagcacg
      121 cggtctttcg atagcgccgc ccgtccgagc agcgacaaaa gacacagcgg tcgactgtcc
      181 gggcccaaca gttcgggcag ataacgtccc aagtcggcgt caggaacccg cacgacgagc
      241 gcttgcaggg ccgcgctgcg gacttccgca tcttcgtctt cgaccagccg ctcgaacagc
      301 gtcacatcgg tttcgcccag ttcatcgagc gcacgcaacg ccgccagtcg tgcgagtgga
      361 tggggatgca aaagcgtcgc acgcaaggga gcccgtcgac cgcccgttcg catcagcgac
      421 tgacaggccc acttgaccgc cccgacgggt gattcattgt ccgacagaat ccggccaatc
      481 agcgcttgcc cccgtcgtcc cgtccggtcc aaaagctcac acgcactttc cagcgcgtgc
      541 ggcagacccg gaatgaccat gtgcggcagc acttcttcca gctcggtcgc tttgatctgt
      601 cgcgccagtc gttcgatggc cagcgtgcgc agcgcggtat gcggatcggc caaaagcggc
      661 agcagctctg ccggtggcac atccgacggt gggcgcacgc gcagtcgggc cgtggtgacc
      721 gccggatcgg gattcgcgag cgactgatcc aaagccggtg gtgccggtgc tttgtaggcc
      781 gcaagctgcg gttcgacgcg cgactgaagt tccaggtggg cgctttgaca cgcctcgttc
      841 atcgtcgccg agacttgcag aatctgttcg cgttcatcgg gaagtgcttg ccacaaaaga
      901 gccagcgctt cgatgccggc gtgacgaacc cgccagaacg gatcaccgag cactgctttc
      961 agcgtcggaa tggcactcgc ctggccaatc cgggccagcg caacggtcgc ggtcctgcgc
     1021 acccaccaca ccgaatcgag cagggccaac cgggtcagtc gtggcagcgt tcgttgtgcc
     1081 ggcagaccgc cgagctgacc gagcgcgcgc gctgcggcct cgcgaacact tggcaaatcg
     1141 tcatacgtcg catcgtgtag ccagggcacc ggtgacaccg cttgggcgcg accgagaaaa
     1201 cgagcggcca acgcgcgcac ccgtccatca ctgtcttcga ggagcgcccg acgtgccgaa
     1261 tacacatccg ccgcgtcctg ttcgtcgagc tgtcccagtg cgatctgccg aacctccgcg
     1321 gaccgagaca gaagcctaat gcgcgcttgc atgccgcctc cttgacgtac cgcgagggtc
     1381 cacaatccca atccccaacg cgactagagt cacgttacca gcgtatgaca caacgctggc
     1441 ttgactcaac gagcaagcac gagcacttgt tcgcgggcgg ccatcaacct gggcgacgcc
     1501 tcgggtgact accagatccg gtgcgtggcg acataaacat ccgaggcaag gtgatcccgc
     1561 gcgtttccgg cagcatccac acccagctcg ccgtggccag cgccagcacc atcggcacag
     1621 tcagcgcgcc cggcagaccg tatcgaactt tcatcgcagc gacgaccacg ggcgccaaaa
     1681 gctggacgga gcgcgccaag ttgtaggtcg ctcccattgc tgctccacga atttccgtcg
     1741 ggaaaagctc ggccaaaagt gcgccaaagc ctgctgtgca acccgaacca aagccgagcg
     1801 ccagcatcgt catccaaaac agcggcggat gttgattgag ccacttccag ttgaacgcca
     1861 aaagtcccag cgccgatgcc gtcagcagtg aatacacgca gaacgccgga cgccgtcccg
     1921 ctcgatccgc caagttgcca aacagcagca tgccgacaaa ctgcccagcc tgtgcggtca
     1981 gcacccacag catgctcttg ccgacgcgct gacccatttc atggagcaaa aacgacggca
     2041 gccaggtgta acaagtccag tacgtgccga tttttagcac cgcgagcacc cacgccagca
     2101 cgaagcgacg accgatgccg gcagagcgaa agacttccga aatgggaacg tggtggtggg
     2161 ctctctcgtt gggaagcacc atcgatcggc gcatggcaac cgcgagcagc gcggtggcac
     2221 tcgacgccag caagatggcg cgccaaccga acaacggcag cagcaaaaac ccggcaacgg
     2281 cggccagtgc ggcgcccagt ggttcacccg cttggaggag ggcggcggct cgaccgcgat
     2341 agcgcgactc cacggcttcg gcgaccaagc catggccaat cgcccattcc ccacccacgc
     2401 caattccgac cagaccgcgg cctatcaaaa acaccgtgag tgacggcgcc agaccgcaga
     2461 ctgccgatcc cagcgaatag acaaacaccg tcagcgccaa aatcgatcgt ttgccgtacc
     2521 tgtccgccag atacccggcg aggattccgc caagtccgga agtcgaaagc gcgacgccca
     2581 gaagccatga ttcgacttgg tgggacaggt ggaactcgtg acttaccgcg tcctttatgt
     2641 aggcaaacag gacgagatcg taaaagtcga aggtccagcc cagaaacgca aacagaaaca
     2701 accgatatgg gaacgggcga cccatgtatc aacttgccct aacttttgag tgggaagagg
     2761 gaaatcggtt gaaacagcgt cggcgacccg tgcggcatga tttatcgatt taagctcgag
     2821 ttgccttttg ggggcaacgg atgagccata tttctttgcg aagccaaaaa agctcgcaag
     2881 cttcggacgt tttttcagac gggaagatgc gtcttgggcc gcgatggttg ctgataagct
     2941 ctgggcgatg acaaaaggtg gcaatctgcg gcttggggtc gatctgggcg gcaccaaaat
     3001 ggaagcggtg gtgctgcggc tcgacggtga atcgttcgaa gtgctggctc gcttgcgccg
     3061 accgaccgac cgagcgcttg gctacgacca catcatcgat gcgctgtgtg gactcatcca
     3121 tgacaccgcg gcgcaggccg gcttggcgga gcttcctccg atcgggatcg gaatgccagg
     3181 cagcatcagc cgccgcagcg gtcttaccaa aaattcgaac acaacctgcc tgaacggcag
     3241 accgtttcgc gccgatgtca tcgccaagct cggccaacct gtgcgttttg ccaacgacgc
     3301 caactgtttc gcgctgtcgg aagcacgctt tggcgccggt cgtggccatc gcgttgtctt
     3361 cggtgtcatc ctcggcacgg gcgtcggcgg cggcttggtt tttcctggcg ctgacgggcg
     3421 tgccgatgcg tgggacggac cgcagggcat tgccggcgag tggggtcacg tcagcctcga
     3481 accgaagacc ggcccggctt gctattgcgg tcgacgtggc tgcatcgaaa cgttcctctg
     3541 cggtcccgcc atcgagtcgc gctacgtcga acgttgcggc agtccgcttt cgctgccgca
     3601 gatcgctgaa cgagtcgctg tcgatgcggt cgcccggcaa gtctgtgacg cccacgtcgc
     3661 gatgtttggc cgggccatcg cgacggtcat caacattgtc gacccagatg tgatcgtgct
     3721 cggcggtggg gtatcgaact tgccttggct atacaccgac ggcgtgaccg aagccgccaa
     3781 gtgggttttc agcgacgaac tcaccacgcc gatcgcaaag caccagcttg gcgatagtgc
     3841 cggagtgatc ggcgcggcgc ttctgccgta acagttttgc tatcctgcgc cgcctatgcc
     3901 ggtgcgcgtt cgtcatcacg tcaatccgtt ccatctccag ttcctcgaca ttgcgccgga
     3961 tcggctttcg gccccgccgg gtagagatcc cgaactgcaa atcgagctgg ggtgtgccga
     4021 tgcgcagttt ctgtttcagc ttgcgcgacg ggaccccttg ggcagctacg tcggcgtcga
     4081 aatccgcaaa gcgcacgttg aagatgtcaa tcgtcgagct gcgaccgagg gactgccgaa
     4141 cctgcgcgcg atttttgcgc acatcaactt ggatctgccg acgctgtttg tgggccatcg
     4201 catccgtcgg tttttcatca attttcccga cccgtggttt aagcgcgcgc accacaaccg
     4261 acggatcgta aatcccgatc tggccaagac cttgacgtcg ctgttggttc ccggcggcga
     4321 gctctttttc caaagtgaca tctgggagct ggggatttcg gcgatggaag tgttcgaaac
     4381 gacgccaggg cttgccaacg tcggcggtga gtgggcgttc caaaagcaaa atccgtatcc
     4441 ggcggtttcg ctgcgcgagg agcgcgtcac cgaggatggc aacccgattt ggcgcattct
     4501 gtaccggcgc gtggcataac cgaaacatga agcgcgcatt cctcggtttg gtcctggccg
     4561 catctgttgt cggctgcgcg ggggatctgg ctggtgccca actggtctat cagctgaagc
     4621 tgcaatccag ccccgtgcca gcgacggcga tgttcgtgga accgcaggtc tgggtggaag
     4681 atccgtactc gacgaagtgg aaggcatctt cgccgattgc tgcggccagc ttggcggggc
     4741 ttaccaagcg cgatcaggtg tcgcttttgc tgcgggtgag ctccgcagat gtggcaagtc
     4801 gggtcgcgcc agttacccgt ttttttgacg acaaacagca gctcgtcgcc gtcggtgggg
     4861 gacagcttgc gctgttcaat gatgccgtct actcgatcac gctcgtcgaa gtcagcgatg
     4921 cgcgactgct ccccctgcaa gtcgccaagt ttgcgcccgc gtccgctttg gccggtcgca
     4981 ccgtcacgat gtacggctgg ggtttttccc ctgaggcggc gatttcgata ggcggccagt
     5041 ccgtgaccga caagcagtgg ctttcgcccg tcgaaatggt ggttcgcatc ccgaacgccg
     5101 ttgccgcagg tccgctcgaa gtgacagtac aaaatcccga cggcacccgc gatgtccgca
     5161 ccgatctgct caccgcgcag tgacaagttc gagcgcgcgg cggattggct gaccaaagcg
     5221 atcctatttg gtgctttttc ccgtcgctga aatcccgtac tgcttcatct tgtacgaaaa
     5281 ggttcggatc ggcatcccga gcagctcagc ggcacgaact tgcacccctt gtgctttggc
     5341 cagcgcttcc atcattcgac gctgttctag ttcgcgaatc tcgtcttcta ggctgcgaaa
     5401 cgtcgggttt ggactgagtc caatttgatc ggccacagcc ggcgttgatg cttggtttcg
     5461 gtcgcggggc tcttgccgca aaagcgacga cacttcggtg ctggtgatga tgccgtcgct
     5521 gctcgtcgct gccagataat cacagaagtt gcgtagctcg cgaatgtttc ccggccaggt
     5581 gtgcgaaagc agcagcgtca gtgcaccgtc agaaaacgcg ggaaccgatc gccccaaatg
     5641 atcggcggcg cggcgcaaaa actgtcgcaa aagcaaaggg atctctcgcc ttcgatcgcg
     5701 cagcggaggc agcatcacgc gggccgcgac caagcggaaa aacaggtctt ggcgaaatcg
     5761 tccctgctgc acttcgtgtt cgagatcgcg atgggtcgca cagacgacgc gaacatcaat
     5821 cggaagttcc cgtgatccac cgacgcgcgt gatcttctgg ctttccagcg cgcggagcag
     5881 cttggcctga agcgacagtg gcagctcgcc cacttcgtcc agaaacagcg ttccctgcgc
     5941 cgcctgttcg agcagcccga gcttctgact gaccgccccg gtgaacgctc ccttttcgtg
     6001 cccaaacagt tcactttccg cgagtgtttc gggcagcgcc gcgcagttga gggcgacaaa
     6061 acgaccaggg cgcttggacc agacatgaag cgccgtcgct gcgatctctt ttcctgtgcc
     6121 cgtttcaccc tgaatcagca ccggaattgg acttttcgcc agccgttcga gcagcgcgta
     6181 aatccgctgc atcgccggat cggcaatggt gacttcatgc ggcccgatcg gctgggtgcg
     6241 gtgcgcttcc gtcgcagaca gacactcgcc gggcttggct aagctgctcg cggtacgggc
     6301 cgacaaaagc aacgaatccg ggtctccacc gtcgaccgga aagctagcca ggccgccacg
     6361 aatcgttttg gcgacgcgca ggacggactg catcagctcg gcaactgtgg tggtccgttc
     6421 atcggcagaa agctctggca gcaaaagcca gacgtgctga tcttcgcgac ttatcacgtc
     6481 gaggagccgt gccgcttggc ccagcaccag cgaaagccgc gcaacatcgg caccagcatc
     6541 gatttgcaga cagagcagcg cgagtggccg accgtaccgc agcgaccgct cgacttcctc
     6601 gctgagccgc tggcgcaaca cgctcatcgg ttgaagttca cgaggacggc tcgaaagacg
     6661 cggcgcatgc agcaccagcg tcacttcgca cagctggatc acatcaccgt gttggagcat
     6721 gcgcgaaccg acgacgcgct ggcccaggac tttggttcca ttgtgcgatc ccaggtcaat
     6781 cagcgtgatg ccatcgtcgt cgatgcgcag gcgagcatga tggcgtgatg ccgtgcgatc
     6841 ggaaagcctg agcccacagt cgtcggcgcg gccaatcagc agctctcccg acggtggcag
     6901 tggacaaagg atcgagctgt tgccttcgac gaccaaaaga tacggcgcag cgtggtgcgc
     6961 ggcttcttgt gctgggtcat tcgggctgac agtcagggtt gcgtcgtcag aaggcgggga
     7021 cattgcgagg gagcgctggg gccgatgaca aaccgatccc gtcggcggtt acttcttgtc
     7081 ttcgtcgtct ttgtaggcgc gcatcacgat gaccgtgatg ttgtcgacgc cgccgttttt
     7141 gttggcttga tcgacgaggt cagcgacgca cttttcgaga tcctcaccgg cggccagcac
     7201 gatcttcagg atcgactgat cgtcaatcat accggtgaga ccgtcgcagc acaggaagat
     7261 cgtgtcgccc ggcagcagcg tgtcgtggcg gatatcgacc tggaccgact cacgcatgcc
     7321 caaggcccgt gtgatgacgt tcttgtgagg gaagtttttc tgctcctcct ccgtcatgtc
     7381 gggct
//
LOCUS       DAORGN010000232         7205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4788_length_7205_cov_6.549650, whole genome shotgun sequence.
ACCESSION   DAORGN010000232 DAORGN010000000
VERSION     DAORGN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4788_length_7205_cov_6.549650"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKL17_18850"
     CDS             complement(<1..757)
                     /locus_tag="PKL17_18850"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46850.1"
                     /translation="MGKSDKPEGRATASGPLSGTVAAKSSGQHSVLPTSKAVGAAKSS
                     GQHAVLVPSRPVSARSLGALLQKHAGLSEVLLREALSEQEDLRRSGGGQTEGTRLGEI
                     LLRRRSVTEEQVLRALGHQFGLDVLLEIRPDTIDQDLVQRIPINFAKQHTVVLLRRNQ
                     ADDTVEAAIADPTAVHVLDDIARVVLAEVSPYLAPAHQILEAINKVYSRAGIDAELQK
                     DEETTEEAETEEDVDLVDVSDEAPIIRWVNSVIF"
     gene            complement(776..3298)
                     /gene="gspD"
                     /locus_tag="PKL17_18855"
     CDS             complement(776..3298)
                     /gene="gspD"
                     /locus_tag="PKL17_18855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNK46851.1"
                     /translation="MLCTGILPAAAQIKAGVAAKPQPAVRPSSPVSPPTSLPPPPTVP
                     PTSGTATSPSVSAAQSAKASTDDLPLPGAAEFLECKAFPPNKKVNFNLKPEAELTDLV
                     GFIKAISCRPIILPQNIRQSKVTVIAPETVTASEAYRIFLSSLESMGLTVQPDGKVLK
                     IIESNRAREASIPVISPGDVVPREERYVTRLLRLKYITADDVVQVLNRLKGRDGDIMP
                     YAAANTLVVTDLASNIRRMEEVVRALDQPMAGEKIWVIRLKNIVATEVLQMLQQVFGV
                     GKASSPGQPRRAPIQVSGGGEAPAPSMVGSGDDSISQIFADDRTNSIILVSAERTYQR
                     VFALIKRLEQQGSTSTGDRIHVYALENANADDMVQVLGGLGISVSAGASRGRSGRGFS
                     PPPQSSGGAQGSSGAFQDEVKVTPDKATNSLVILATGKDFLTLRDVIRRLDVPRRQVF
                     IEAMVMEVSISKSRDLGLSYHGAIPGLGGLVVGGLQAGNLRSLSPLSLLSAQGLVGGL
                     VGPQITGSTIFGSGTGGSALPPQFGILLQALQTNNDVNVMQVPNILTTDNEKSTITVG
                     QNLPFPSAITGGLPMAGQSGSMAGFAALQSVQRQDVQLKIEVTPHVSASETVRMELKT
                     EISDVAADNYNNLGPATNKRTLETTAVVRDQQPVVLGGIMRDKVSEAVTKIPILGDIP
                     ILGYLFKTRNKKIDKQMLLIVLTPYVINDPSELSRIFERKTRERREFLEAYATFSDER
                     DLVGPIDYTKKRGALEEINRTAVESERELVELRTAEQSLHREVVDSGPVELPPGTRSN
                     ASPGATQTVSPSVSSTTQSPSSPPPPATVKPIAPPPPGAPPG"
     gene            complement(3344..4273)
                     /gene="gspC"
                     /locus_tag="PKL17_18860"
     CDS             complement(3344..4273)
                     /gene="gspC"
                     /locus_tag="PKL17_18860"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HNK46852.1"
                     /translation="MLKNYFWVINLLVVGICSALLGRTTANSVVAALLAGDGPAFPRR
                     DISSGASTEKIRSKDIESILKRNIFCSGCAPISLTPDKAQQDEGPVDQTPQRSGLPLQ
                     LISTMVSPGDWDWSMAVIRDTSDDRKRVGIFRRGDKLTQGGAIVAQVVEGRVYMVVGK
                     RVEYITSDKEPPPPPPTAVATAEPSTTDPTGIEKDVEKGVRCTGSSCEVDKSLIDKVL
                     ANTTALATSARFVPWIKDGKPSGFKLYNIRTNSIFSKIGIQNADLIKAINGLDMSTPD
                     KALEAYTKLKSASHLTVMVERRGENVTLDYQIR"
     gene            complement(4517..5356)
                     /locus_tag="PKL17_18865"
     CDS             complement(4517..5356)
                     /locus_tag="PKL17_18865"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK46853.1"
                     /translation="MQVHHIRSATMLLSIGPHHLLVDPMLSPPGALPGFKLFGGGRTA
                     NPLVPLPQNTDDILAQTTGVVVTHEHPDHLDPAAVAWISARKLPVWASPTDVPSLRKK
                     GLSANELKSGELGLAVELIPSLHGPGVVGWLMGPVAGFYLAHPDEPSVYLTSDSVLSP
                     GVLGALARLRPELVIAPAGSANFGLGPSILFTQDELVRLIRDAPHDVLLNHLEALDHC
                     PTTRQALRARLEKEGLSSRVHIPADGETVSFVRKQKTPHPAPGPGLVQVPRLQKWVSS
                     LLG"
     gene            5464..6342
                     /locus_tag="PKL17_18870"
     CDS             5464..6342
                     /locus_tag="PKL17_18870"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNK46854.1"
                     /translation="MQWDLLRTFEAVARLGSLSSAAKALGQSQSTVSRHIGKLEALSG
                     SPLFLRTNPVKLTERGEALIQAVLPMVSASHEAALAIQDSPKLHGDVTLTTVGEILRW
                     VLCRHLPSFYRDYPHLRLHILADNRVSSLAQGEADVALRLARPSRGELTARKLHTESY
                     GFYAASSVNLHKDVPWLGLTGSLASIPEQRFADRTFASRKARLLVEDIETLAQSVALG
                     LGVAILSRSVAARLPGLREVLPQQVGGQDLGPIPTRDFWIVVHRRKQRIPKVRAVMTW
                     LLGIPSFDVREAPTHR"
     gene            6456..>7205
                     /locus_tag="PKL17_18875"
     CDS             6456..>7205
                     /locus_tag="PKL17_18875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46855.1"
                     /translation="MLASISEGVLLERARAQGLLSVDDMAAAQKEQGRHQVVRKWGPV
                     LDLLIAQGKISDEAVRKLHASLQEQSPMSLQPALDRTMDGGLEHTMQSEAGVVVMPQV
                     VSAQTYPAPHWDKYEFIETLGRGGMGAVYKARDRRLGRMVALKFIHGDDPAMIQRFLQ
                     EARAQARLDHPFICKVYEVGSVDNKPYIAMELVEGRTLDRASAGLDLSQKVQLLKDAA
                     EALHAAHQQGIIHRDVKPSNIMVERTATGGLR"
BASE COUNT         1375 a         2264 c         2133 g         1433 t
ORIGIN      
        1 gaaagatcac cgagttgacc cagcgaatga tcggcgcttc gtcgctgaca tcgaccaggt
       61 cgacatcttc ttcggtctct gcttcttcgg ttgtctcttc gtctttttgc agctcggcat
      121 caatgccggc acgcgaatag actttgttga tcgcttccag aatctggtgc gctggcgcca
      181 aatacggcga cacctcggcc aaaacgaccc gcgcaatgtc atccagcaca tgcaccgcgg
      241 tgggatctgc aatcgcggcc tcgacggtgt catcggcttg attgcgacgc agaagcacga
      301 cggtgtgctg cttggcaaag ttaatcggaa tccgctgcac caagtcttga tcgatggtgt
      361 caggccggat ttcgagcagc acatccagac caaactggtg cccgagcgcg cgcagcacct
      421 gttcttccgt caccgatcgc cgacgcagga gaatctcgcc aagccgcgtg ccttcggtct
      481 ggccaccgcc ggaacgccgc aggtcttcct gttcgctgag cgcttcccgc aagagcactt
      541 ccgacagccc tgcgtgtttt tgcaagagcg ccccaagcga gcgagccgac accggtcgcg
      601 aaggcacaag gaccgcgtgc tgccccgagc ttttcgccgc accaaccgcc ttgctcgtcg
      661 gcaacaccga gtgctggcca gaacttttgg cagcgacggt gccactgagc ggcccacttg
      721 ccgtggctct gccttctggt ttgtcggact tgcccatgtg tttcctcgct tgccgttagc
      781 ccggaggcgc ccctggtggt ggtggcgcaa tcggtttcac cgtcgctggc ggcggtggcg
      841 agctaggcga ctgcgtggtg ctcgaaacag acggactcac cgtttgggtc gcgcccggcg
      901 aagcatttga acgggttccc ggtggcagct cgaccggacc cgaatcgacc acttcacgat
      961 gcagcgactg ctcagcggta cgcagctcga ccagctcgcg ctcgctctcg accgctgtgc
     1021 gattgatctc ttcgagcgcg ccccgtttct tcgtgtagtc aatcggcccg acgaggtcac
     1081 gctcatccga aaacgtcgcg taggcttcca aaaactcgcg ccgttcccgc gtcttgcgct
     1141 cgaaaatccg cgacagctcc gacggatcgt taatgacata cggcgtcagc acgatcagca
     1201 gcatctgctt gtcgatcttc ttgttccggg tcttaaacag atagcccagg atgggaatgt
     1261 ccccgaggat cggaatcttg gtcaccgcct cggacacctt gtcgcgcatg atgccgccaa
     1321 gcaccaccgg ctgctgatca cgcaccaccg cagtcgtttc cagcgttcgc ttgttggtcg
     1381 ccggacccag gttgttgtag ttgtcagcag cgacgtcgga aatctcggtc ttcagttcca
     1441 tccgcaccgt ttccgatgcg ctgacgtgtg gcgtcacttc gattttcagc tgcacgtctt
     1501 ggcgctgcac cgactggagc gcggcaaagc ccgccatgct gcccgactga cccgccatcg
     1561 gcagaccacc ggttattgcg cttgggaacg gcaggttttg accgacggtg atcgtcgatt
     1621 tttcgttgtc cgtcgtcagg atgttcggca cctgcatgac gttgacgtcg ttgttggtct
     1681 gcagcgcttg cagcaagatg ccaaactgcg gcggcagcgc cgatccacct gtgcccgaac
     1741 caaaaatcgt gctgccggtg atctgcggcc cgacgagacc gccgaccaga ccttgcgccg
     1801 acagcagaga cagcgggctc agcgaacgga gattgcccgc ttgcagacca cccaccacca
     1861 gaccgcccag acccggaatc gcgccgtgat acgacagacc cagatcgcgg gatttggaaa
     1921 tgctgacttc catcaccatc gcttcgatga agacctggcg acgcggcaca tccaggcgac
     1981 gaatcacatc gcgcagcgtc aggaagtctt ttcccgtcgc caagatcaca agcgagttgg
     2041 tcgctttgtc cggcgtcact ttcacttcgt cttggaatgc accggagctg ccttgcgcgc
     2101 caccactcga ctgcggcggc ggactaaagc ctcgacccga acgaccgcgg cttgccccag
     2161 cggaaaccga aatgccaagt ccgcccagca cctgcaccat gtcatcggca ttggcatttt
     2221 ccagcgcgta gacgtgaatg cggtcgccag tggacgtcga tccctgctgt tccagccgct
     2281 tgatcagcgc aaacacccgc tgatacgtgc gctccgctga caccaagatg atcgaattgg
     2341 tgcgatcgtc ggcaaatatc tggctgatcg aatcgtcgcc gcttcccacc atcgacggag
     2401 ccggcgcctc accgccaccg ctgacctgga tcggagcccg ccgcggctga ccgggtgagc
     2461 tggctttgcc gacgccaaac acctgctgaa gcatctgcag cacttcggtc gcaacaatgt
     2521 ttttgagccg aatcacccag attttttcgc ccgccatcgg ctgatcgagc gcacgcacga
     2581 cttcttccat gcggcgaatg ttcgacgcca ggtcggtcac caccagcgtg ttggccgccg
     2641 catacggcat gatgtcaccg tcgcgaccct tgagccggtt tagcacctgc accacatcgt
     2701 ctgcggtgat gtacttcagt cgcaaaagcc gcgtcacata gcgttcttcg cgcggcacca
     2761 catcgccggg gctgatgacc ggaatcgacg cttcacgcgc gcggttcgat tcgatgatct
     2821 tcagcacttt gccgtccggc tgcacagtca gtcccatcga ctccagcgac gacaggaaga
     2881 tgcgatacgc ttccgacgca gtgacggtct ccggcgcgat cacggtcact ttcgactgcc
     2941 gaatgttctg cggcaaaatg atcgggcggc acgaaatcgc tttgataaag cccaccaggt
     3001 ccgtaagttc agcctcgggc tttaggttga agttgacctt tttgttcggc ggaaaggcct
     3061 tgcactccag aaactccgcc gcgcccggta gtggcagatc gtccgtgctc gctttggcag
     3121 actgggccgc ggatacactc gggctcgtcg ccgttccgct ggtcggcgga accgtcggtg
     3181 gtggaggcag agaagtcggc ggtgacaccg gactgctcgg acgaaccgcc ggctgtggtt
     3241 ttgccgcaac tcccgctttg atctgagccg ccgccggcag aatcccggta cacagcatca
     3301 gagcggaact acatattatg cgcttgaaag tattcatatt cggtcaccga atctggtaat
     3361 cgagcgtcac gttttcgcca cgccgttcca ccatcaccgt caggtgggaa gcgcttttga
     3421 gcttggtata agcctcaagg gctttgtctg gagtcgacat atcaagaccg ttgatggcct
     3481 tgatgagatc cgcgttctga atcccaatct ttgaaaagat cgagttggtg cggatgttgt
     3541 aaagcttaaa tcccgacggc ttgccgtctt tgatccacgg gacaaagcgg gcacttgtcg
     3601 caagcgccgt ggtgttggcc aacactttgt cgatcaggct cttgtcgact tcgcagcttg
     3661 accccgtgca gcgcacgccc ttttcgacat ctttttcgat cccggtcgga tcggtcgtgc
     3721 tcggttcagc cgtagccact gcggtcggcg gtggcggcgg tggttccttg tcgctggtga
     3781 tgtattcgac gcgcttgccg acgaccatgt agacccggcc ttcgaccacc tgcgcgacaa
     3841 tcgccccgcc ctgcgtcagc ttgtcaccgc gacggaaaat accgactcgc ttgcggtcat
     3901 cgcttgtgtc gcgaatcacc gccatcgacc agtcccagtc gcccggcgac accatcgtcg
     3961 aaatcagctg gagcggcagg ccgctgcgct gtggcgtctg gtcgacgggg ccttcgtctt
     4021 gctgggcctt gtccggcgtc agcgaaatcg gcgcgcagcc cgaacagaaa atgttccgct
     4081 tgaggatcga ctcaatgtcc ttgctgcgga tcttttcggt gctggcaccg ctgcttatgt
     4141 cccgacgagg aaacgcagga ccgtcccccg caagcaaggc cgcgaccacc gaattggccg
     4201 tcgtccgtcc aagcagcgcc gaacagatcc cgacgaccag caggtttatg acccaaaagt
     4261 agttcttgag cacggcttct cactccgcct gccaagtaac actgcaatcc acgggccatc
     4321 gcgcaaagcc tcgctgaacg ggcgtctcgt cgaggatctg gcgtgaccgc gtacaatcgg
     4381 actgtcagtt tggcaagcgg tcgccccgcc gggcctgtcc gcgttgccaa attgacaagc
     4441 ggcgcggcgc caaagcgatc tcgcaaggtc gccgccgaat cgatgtgggc tgggcgccgg
     4501 ggcccggcag acgatgctag ccgagcagcg acgacaccca cttttgcaga cggggcacct
     4561 gcacaagccc cggtccgggc gctggatgcg gggtcttttg tttgcgcaca aacgacaccg
     4621 tttcgccgtc ggcaggaata tgaacccgag acgaaagccc ctctttttca agccgcgcgc
     4681 gcagggcctg ccgcgtggtc ggacagtgat cgagcgcttc caggtgattc agcagcacat
     4741 cgtgtggcgc gtcgcgaatc aagcggacaa gctcatcttg cgtaaacagg atcgatggac
     4801 ccagtccaaa gtttgcgctc cctgccggcg caatcacaag ctctggacga agccgcgcaa
     4861 gtgccccgag cacgccaggg cttagcaccg aatccgacgt caagtacacg ctcggttcgt
     4921 cgggatgcgc caggtagaag cccgccaccg gacccatcag ccaacccacg acgcccggac
     4981 cgtgaagcga cggaatcagc tcgactgcga gccccagctc cccacttttg agctcgtttg
     5041 ccgaaagtcc ttttttgcgc aggctcggca cgtcagtcgg acttgcccac actggcagct
     5101 tgcgcgccga aatccatgcc accgccgccg ggtccaggtg atccgggtgt tcgtgcgtca
     5161 ccacaacgcc cgtcgtctgc gcaagaatgt catcggtgtt ttgcggcagc ggcacaagcg
     5221 gattggccgt tcgcccacca ccaaacagct tgaagccagg aagcgcccct ggtggcgaca
     5281 gcatcgggtc gaccagcaga tggtgtggac ctatcgaaag cagcatggtt gcgctgcgga
     5341 tgtgatgtac ctgcatcggg tttggcctcg cgctttgggt ttattccatg gtggcgtctt
     5401 gcgaaaatgg atggcaatcg gcaaatctgc acacaagtca tccgtttttg catggagggt
     5461 gtcgtgcagt gggatcttct tcggacattt gaggccgtgg cgcggcttgg cagtctttcg
     5521 tccgcggcca aagccctggg ccagagtcag tcgacggtga gcaggcacat tggcaagctc
     5581 gaagcgctct ccggttcacc gctgtttctg cgcacaaacc ccgtgaagct gaccgagcga
     5641 ggggaagcgc tgatccaagc ggtgctgccg atggtcagcg cgtcgcacga agccgccttg
     5701 gcgatacaag attcgcccaa gctgcacggc gatgtgacgc tgacgaccgt gggagagatc
     5761 ctgcgctggg tgctgtgccg ccacctgccg tcgttttatc gcgactatcc acacttgcga
     5821 ctgcacatcc tcgcggacaa tcgcgtaagc agtctggcgc agggagaagc cgatgtcgcg
     5881 ctgcggcttg cacggcccag ccgcggcgag ctgaccgccc ggaagctcca caccgagtcc
     5941 tatggttttt atgcagcgtc gagtgtgaat ctgcacaaag acgtaccgtg gctggggctc
     6001 accggatcgc ttgctagcat tccggaacag cgctttgcag accgcacgtt tgcaagccgg
     6061 aaggcgcgcc tgctcgtcga agacatcgaa acgctcgcgc agtcggtggc cctgggcctg
     6121 ggggttgcga ttctgtcgcg cagtgttgcg gcgcggctgc ccggcctgcg cgaagtgttg
     6181 ccccagcaag tgggcggcca ggatctgggg ccgattccca cacgcgattt ctggatcgtg
     6241 gtgcaccgac gaaaacagcg cattcccaaa gtccgtgcag tcatgacgtg gctgctgggg
     6301 attcccagct ttgatgtgcg agaagcgccg acgcataggt aatgtccgat cgacgagcag
     6361 acccggcgcg gtcacgaatc gacagactgc ggcccgacca gctttcctgc ctacagacaa
     6421 aacgcggtat gctgttcttt ccgcgaaagt gcctgatgct ggcttcgatc tctgaagggg
     6481 tactgctcga gcgcgcacgc gcacaaggtc ttctgtcggt ggacgacatg gcagcggcgc
     6541 aaaaagagca aggccgtcac caagtcgtcc gcaagtgggg tccggtgctc gatctgctga
     6601 tcgcgcaggg taaaatttcc gatgaagcgg tgcgcaagct gcatgcgtcg ctgcaagaac
     6661 agtcccccat gagcctacag cccgcgctag atcgcacgat ggacggcggg cttgagcaca
     6721 cgatgcagag cgaagcgggc gtggtggtga tgccacaggt cgtctctgca cagacgtatc
     6781 ctgcgccgca ctgggacaag tacgagttta tcgaaacgct ggggcgcggc ggcatgggag
     6841 ccgtctacaa agcgcgcgat cgccggctgg gacgcatggt cgcgctgaag tttatccatg
     6901 gcgatgaccc agcgatgatc cagcgattcc ttcaggaagc acgagcccag gcgcgtcttg
     6961 atcatccctt tatttgcaaa gtctacgagg tcggttccgt cgacaacaaa ccatacattg
     7021 cgatggagct ggttgaaggc agaacgctcg acagggccag cgccggtctt gatctttcgc
     7081 aaaaagtaca gctcctcaaa gacgctgccg aagccctcca cgccgcccac caacaaggaa
     7141 tcatccacag agacgtgaag ccgtccaaca tcatggtgga acggacggct acgggtgggc
     7201 tgcgt
//
LOCUS       DAORGN010000233         7172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4821_length_7172_cov_6.215259, whole genome shotgun sequence.
ACCESSION   DAORGN010000233 DAORGN010000000
VERSION     DAORGN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_7172_cov_6.215259"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1457)
                     /locus_tag="PKL17_18880"
     CDS             complement(213..1457)
                     /locus_tag="PKL17_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK46856.1"
                     /translation="MSGSPPARRNLAILFLLYFAQGLPFGFQKELSAFLRQAGLSLTK
                     IGLSRGLAAPWLLKALWAPLVDRYGLPDFGRRKSWIVPLQLLLALVFVAAAFVPPHVS
                     LPTLIALVFLMNILTATQDIAVDGLAIDLFKGQQVGGANVAQVVGYKIGMLVGGGVLL
                     RASASIGWRGMLFALAALVVVVLLAVLTLREPKVKPEASHVQQHLADILATLGRQLQS
                     PAGRWLILFVGTYKIGETLVDALYSSFLIDSGYTPAQIGGWLGIWGMVASTLGSLTGG
                     FFASRLPILTAVALTSLLRVAAMGGECYLTFLSPPLPADILGVSLAEHFAGGALTTAM
                     FAFMMMRVDKRIGASHYTVLASIEVLGKMPTGLFAGVMATHIGYRATFLLGTALSAVF
                     LLLLIPLRKLPAVSPLDADSAS"
     gene            complement(1476..2477)
                     /gene="pip"
                     /locus_tag="PKL17_18885"
     CDS             complement(1476..2477)
                     /gene="pip"
                     /locus_tag="PKL17_18885"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNK46857.1"
                     /translation="MTPSPLPAATTPSTLLTFFPLHEPYRTGFLRVSPLHELYFEESG
                     NPQGQPVLFIHGGPGSGTEPNQRRFFDPRHYRIILFDQRGAGRSRPHASVEDNTTWDL
                     VADIEKLRAHLHIDKWVVFGGSWGSTLALAYAETHPEQVCGLIVRGICLLTKNEIDWL
                     FRKGASMLFPESWDDFKSFIPKDEQSDLLAAYHRRLHGPDRAVQQSAAVAFARWEACL
                     SRLLPDPHVIARFAESSFAYALSRIESHYFVNDGFFAHDGQLLTDAHRLGKVPTVIIQ
                     GRYDLVCPMESAYLLHRAVPHADFFIVNDAGHSATEPGITHRLIEATERMKLAKKSA"
     gene            complement(2539..4107)
                     /locus_tag="PKL17_18890"
     CDS             complement(2539..4107)
                     /locus_tag="PKL17_18890"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNK46858.1"
                     /translation="MLFLLSGSVAAEKTPAAAPVPTYAAINPDSEDDKEAESDQDTPR
                     RALERFLAQTSAGDYFKAAEAIPVSSTNVGDGAEKARRLALVIERRMNTSPDELAKVS
                     DLPAGNQTDGLKHQDVIGRIDMPLGSETIRLQRQGTPGQRRWVFSPATVARIDAWYER
                     LEDHFVLETLPEALLNRGPLGLAYWQWLALPFLLILSGLGGIALSVMLRPLLRKLLRN
                     HELWLAMLERQRGPLWLLFGALFALLLLPLLYLTPTVEQPFRAITHIAMEVSICWIAW
                     RIADVLTTRARLLEWLEARPGLRGILPLIRQLIGIGLTAIAAAIALSELGLSLTSVMA
                     SLGLGGLAVALAAKNTLEHFFGSVTLSIDQSIRVGDQVRIGDVTGIVEQIGMRSTRVR
                     TADRSVVTIPNGKVADMQIETLAARDRTRFFLSLSVVCALKPAGLRNLRDALVKCVRS
                     HPKVWPDVVRVHFTGLTDSTLPVEITAWFQVTEQDQFLEARQELLLQILEVIEQFGAT
                     PAFAKASAPPSSGR"
     gene            4281..5225
                     /locus_tag="PKL17_18895"
     CDS             4281..5225
                     /locus_tag="PKL17_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46859.1"
                     /translation="MWRGSAETALWVVTTYFNPAGYAARRHNHQRFCAALGAVPCLTI
                     ECAERDRTFELAPGPNVVQVRAADTLWHKERLINLALCRLPPSCRFVAWVDGDVLFEN
                     PDWQKHAMELLGDKCAVQLFSDVALMPSPLAAQHDEKPQVVDGFAAKTSRDPESLRAG
                     RFDAHGHSGFAWAMRRDVLSEVGLYDACIVGSADHVMAHALLDGPFATCVTRLLGLGT
                     PHHRHFVAWADRLRASIGQRRLAGIGAVPGRILHLWHGSIADRRYFDRNQRLLRLGFD
                     PRVDLLDEPGQPWRLRDPGGELAAFLRDYFAQRNEDSL"
     gene            complement(5247..6608)
                     /locus_tag="PKL17_18900"
     CDS             complement(5247..6608)
                     /locus_tag="PKL17_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46860.1"
                     /translation="MPSSVGRLALATLLGFGLVAGAVQVGEANEPSRNAPFPPLRSAQ
                     QKPTWLGGAPAGGSAAAVSQAATAEAPSLPIGSPTRNAPASASSDGVLPDAKWCGIEP
                     QSTQPEPTNPCKLTTLPCGPIGSDKIHNGSGEDTDDLDGDGQRDLVIGGRISVTAKEV
                     FSAIYRGTENGYLLSDYRIVPPRAEPTFASVLLTENGRTPLLRDGHDLVESSGRSLSI
                     ARLRRFDGQRFRTLLTFCAHRSEPSPSAPGGVRQGLNRVDFIDVDKDGNKEVVIQGLL
                     APVVFRFADTGLLLLEDRALTQTYRETSHEAQRARSLRAEAEKLIDEGEVRRAAETYQ
                     RAWLSAPYDIELGLSAADAQVRAGQPDRGLDMVTRLRYQAPDRGTIYCTIGAVQKALR
                     NTAGEQAALKVCAEKETDEVRRRQAQNRLRELAGLPVVQPQVVAPKDSSAQSILDEGL
                     GTP"
     gene            complement(6622..>7172)
                     /locus_tag="PKL17_18905"
     CDS             complement(6622..>7172)
                     /locus_tag="PKL17_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNK46861.1"
                     /translation="RDGWDRCELLFSANHGEDPRPLQRIASGGELSRVMLALRGVLGQ
                     ADEVATCVFDEVDSGIGGATADRVGCKIRSLANGKQVLCISHLAQIAAYGQHHFRVEK
                     QVLSGRTVTSVRPLRPTERRDEIARMIGGARLTEKSRAHADELIAHAQLAGVLRSAPQ
                     ASQPNPSRDSRSRRTLKADARA"
BASE COUNT         1350 a         2098 c         2436 g         1288 t
ORIGIN      
        1 gatcgatgcc gccgcagctt agggccaggc ccgacgcgca caggagggga aagaaacgcc
       61 gggtaaggga aagctgtttt ttcattgctg acacctcgtt tggggaaaaa cccagttgcc
      121 tgacacggga cagccgaaaa atttttcggg gagccgggta gggcgcggac gaagcgcgaa
      181 tcgttacaaa ggcgcaaaaa tcgcgtcaag cactaggacg cggaatctgc atcgagggga
      241 gagacggcgg ggagtttgcg cagtggaatg agcagtagca aaaacaccgc cgacagcgca
      301 gtaccgagga gaaacgtcgc gcgatagccg atgtgggtgg ccatcacgcc ggcgaagagt
      361 ccggtcggca tcttgccgag cacttcaatg ctggcgagca cggtgtagtg actggcgccg
      421 atccgtttgt cgacccgcat catcatgaac gcgaacatcg cggtggtcag ggcgccaccg
      481 gcaaagtgtt cggcgagcga caccccgagg atgtcggcag gaagcggcgg cgacaaaaac
      541 gtcaggtaac actcgccacc catggcagcg acgcgcaaaa ggctggtcag cgcgacggcg
      601 gtcaggatgg gcagtcgact ggcaaaaaaa ccgccggtca gcgacccgag ggtcgatgcc
      661 accatgcccc aaatcccgag ccagccgccg atttgtgccg gggtatagcc gctgtcgatg
      721 agaaacgagg agtagagcgc gtcgaccagg gtttcgccga ttttgtaggt gccgacaaac
      781 aggatgagcc agcgtccggc ggggctctgg agctgacggc ccagcgtcgc gaggatgtcg
      841 gccaggtgct gctgaacgtg gctggcctcg ggtttcactt tcggttcgcg cagggtcaag
      901 accgccagca gcacgacgac gacaagcgcc gcgagggcaa acagcatgcc gcgccagccg
      961 atgctggctg aagcgcgcag aagaactccg ccaccgacga gcatcccgat cttgtaaccg
     1021 acgacctggg cgacgttggc gccgcctacc tgctggcctt tgaacagatc aatcgccagt
     1081 ccgtcgacgg caatgtcctg ggtcgcggtg aggatgttca tcaaaaacac cagcgcaatc
     1141 agggtcggca agctgacgtg cggcggcacg aacgcggcgg cgacaaacac caaggcgagc
     1201 aaaagctgga gcggcacgat ccacgacttg cggcggccaa agtcgggcag gccgtagcgg
     1261 tcgacaagcg gtgcccacag cgctttgagc agccacggtg ccgccaggcc gcgcgaaaga
     1321 ccgatcttgg tcagcgacag acccgcctgg cgcaaaaaag ccgacagctc tttttgaaag
     1381 ccgaacggca ggccttgggc aaagtagagc agaaatagaa tcgcgagatt gcgacgcgcc
     1441 ggaggagagc cgctcaccct cctatcctag cgtgcttagg cggatttttt ggcaagcttc
     1501 atgcgctcgg tggcttcgat caagcggtgg gtgatgcccg gttcggtggc cgagtggccg
     1561 gcgtcgttga cgatgaaaaa gtcggcgtgc ggcacggcgc ggtggagcaa atacgccgat
     1621 tccatcggac acaccaggtc gtagcgcccc tggatgatca ccgtcggaac tttgcccaag
     1681 cgatgcgcgt cggtgaggag ctggccatcg tgcgcaaaga agccgtcgtt tacgaaatag
     1741 tgcgactcga tgcgggacag cgcatacgcg aagctgcttt cggcaaaacg cgcgatgacg
     1801 tgcgggtcgg gcagaagccg gctcaggcac gcttcccagc gcgcaaaagc gacggcggcg
     1861 gactgctgca cagcgcggtc gggtccgtgc agccggcggt gatacgcagc gagcaggtcg
     1921 ctctgttcgt ccttggggat gaacgacttg aagtcatccc acgactcggg gaacagcatg
     1981 ctggcgcctt tgcggaacag ccagtcgatt tcgtttttgg tcagcaagca gatgccgcgg
     2041 acgatcaggc cgcagacctg ctctgggtgc gtttcggcat aagcgagcgc gagcgtactg
     2101 ccccaagaac ccccaaatac cacccactta tctatgtgaa ggtgcgcacg cagtttttcg
     2161 atgtccgcga ccagatccca ggtggtgttg tcttccacgc tggcgtgagg ccgactgcgc
     2221 ccggcgccgc gctgatcaaa caagatgatc cggtagtggc gcgggtcgaa aaaccgtcgc
     2281 tggttgggtt cggtgccgct gcctggtccg ccgtggataa acagcaccgg ctggccttgc
     2341 ggattgccgc tctcctcgaa atacagctcg tgaaggggcg acacgcgcag gaagcctgtg
     2401 cggtacggct cgtgcagcgg gaagaaggtc aggagggtgc tgggagtcgt cgcagctggc
     2461 aaagggctgg gtgtcattag gtaaagcgtg gcagttttac cttgggaagt caaattcccg
     2521 ttatgggcat tgcaaaaatt atctgccaga agagggcggg gcgctggctt tggcaaacgc
     2581 tggggtcgcg ccaaactgct cgatgacttc caaaatctgg agcagaagtt cctggcgcgc
     2641 ttcgaggaac tgatcctgct cggtgacttg gaaccaagcg gtgatctcga cgggcagggt
     2701 cgaatcggta aggccagtaa agtggacgcg cacgacatcg ggccagacct tgggatggct
     2761 gcgcacgcac ttgacgagcg cgtcgcgaag gttgcgcaga ccggcgggct tgagcgcgca
     2821 aacgacggaa agcgacagga aaaaacgggt gcggtcgcgg gcggcaaggg tttcgatctg
     2881 catgtcggcg actttgccgt tgggaatggt gacgaccgag cgatcggcgg tgcggacgcg
     2941 ggtcgatcgc atgccgattt gttcgacgat gccggtgacg tcgccgatgc ggacttggtc
     3001 gccgacgcgg atcgactggt cgatgctcag cgtgacgctg ccaaagaagt gctcgagggt
     3061 gttttttgcg gcgagggcga cggcgagacc gcccagaccc aagctggcca tcacgctggt
     3121 gagcgacaag ccaagctcag acagcgcaat cgcggcggca atcgcggtca ggccaatccc
     3181 gatgagctgc cggatgagcg gaagaatgcc gcgcagtcca ggccgcgctt cgagccactc
     3241 cagaaggcgg gcgcgggtgg tcagcacgtc ggcgatgcgc caggcgatcc agcagatcga
     3301 cacttccatc gcgatgtggg tgatcgcgcg gaagggctgc tcgacggtgg gcgtcagata
     3361 caaaagcggc agaagcagaa gcgcaaacag cgcgccaaac aaaagccaca gtggtccgcg
     3421 ctggcgttcc agcatcgcca gccacagctc gtggttgcgc agcagcttgc gcaaaagcgg
     3481 acgcaacatc accgacagcg caatcccgcc gagtccgctc agaatcagca aaaacggcag
     3541 cgccagccac tgccagtaag ccagcccgag cggcccgcgg tttaggagcg cttcgggcag
     3601 ggtttccagc acaaagtggt cttcgagccg ctcgtaccac gcatcaatgc gcgccaccgt
     3661 cgcgggtgaa aacacccagc ggcgctgacc cggcgtacct tgccgctgga gccggatcgt
     3721 ttccgaaccg agcggcatgt cgatgcgccc gatcacatcc tggtgcttga gcccatcggt
     3781 ctggttgccc gccggcagat cggacacttt cgcaagctca tcggggctgg tgttcatccg
     3841 ccgctcgatc accagcgcga gccgacgcgc cttttccgcc ccatcaccga cgttggtcga
     3901 cgaaaccggg atcgcttcgg cggctttgaa ataatccccg gcgctggttt gggccaaaaa
     3961 tcgctcgagc gcccggcgcg gcgtgtcctg gtcgctttcg gcttctttgt cgtcttcgct
     4021 gtccggattg atcgcggcgt aggtcggaac aggcgcggcg gcaggcgttt tttcggcggc
     4081 gacgctcccg gaaagcaaaa acaacatcgc caggcttgcg aggatcgcgc accgggcggg
     4141 ggcgggacgg actgtgcgga cacaactttg catcgttaga gcatcccgaa agagccggca
     4201 aagctcaatc ccatggtcaa ttccgtcgga cacttgcagg ccaagtgacg tcggcgagcc
     4261 ggcttgcgat agtggggcgg atgtggcgcg ggtctgcgga aacggctttg tgggtggtga
     4321 cgacgtactt caacccggcg gggtatgcag cgcggcggca caaccaccag cgtttttgcg
     4381 ccgcgctggg cgccgtgccg tgtctgacca tcgagtgtgc cgagcgcgac cgcacatttg
     4441 agctggcacc aggcccaaac gtcgtgcaag tgcgggcagc cgatacgctg tggcacaaag
     4501 agcggctcat caacttggcg ctttgccggc tgccaccaag ctgtcggttt gtcgcgtggg
     4561 tcgatggtga tgtgctgttt gaaaatccgg actggcagaa gcacgcgatg gaactgctgg
     4621 gggacaagtg cgcggtgcag ctcttttccg acgtggcgct tatgccgtcg ccgctggcgg
     4681 cgcagcacga cgaaaagccg caagtggtcg atggttttgc cgcaaagaca agccgcgatc
     4741 cggaatcact gcgcgctggg cgattcgatg cccacggcca cagcggcttt gcctgggcga
     4801 tgcgccgcga cgtgctgtcc gaagttggtc tttatgacgc ctgtatcgtc ggcagcgcgg
     4861 atcatgtgat ggcgcatgca ctgctcgatg ggccttttgc gacatgtgtg acacggcttt
     4921 tgggcctggg gacgccgcat catcgccact ttgtcgcctg ggccgatcgt ctgcgcgcgt
     4981 cgattggtca gcgtcggctg gcgggaattg gcgctgtacc ggggcggatt ttgcacctgt
     5041 ggcacggatc gatcgccgac cgtcgctact tcgatcgcaa ccagcgtctt ctgcggctgg
     5101 gttttgaccc gcgggttgat ctgctggacg aaccgggaca gccgtggcga ctgcgggatc
     5161 caggcggcga gctggcggcg tttttgcgtg actattttgc gcagcgcaac gaagacagtc
     5221 tgtagcttgt gcggcgcagg cggcgtttac ggcgttccga gcccttcgtc gaggatcgac
     5281 tgcgcactcg agtctttggg tgcgacgact tgcggctgca ccaccggcag accggccagc
     5341 tcgcggagtc ggttttgcgc ttggcggcga cgcacttcat cggtttcttt ttcggcgcag
     5401 accttgagag cggcttgttc tccggcggtg ttgcgcagcg ctttttgcac cgcgccgatc
     5461 gtgcagtaaa tcgtgccgcg gtcgggggcc tggtagcgca gcctcgtcac catgtcgagc
     5521 ccgcgatcgg gctgaccggc gcgaacctgg gcgtccgctg ccgatagtcc cagctcgatg
     5581 tcatatggtg cactgagcca cgcgcgctgg tacgtttcgg cggcgcggcg cacttctcct
     5641 tcgtcgatga gcttttcggc ttcggcgcgc aggctgcggg cccgctgcgc ttcgtggctg
     5701 gtttcgcgat acgtctgggt gagcgcgcga tcttcgagca gcagaagccc ggtgtcggca
     5761 aagcgaaaga ccaccggagc aagcagccct tggatcacga cttctttgtt accgtctttg
     5821 tcgacgtcga taaagtcgac gcggttgagt ccttggcgaa cgcctccggg cgcgcttggc
     5881 gacggctcgc tgcggtgggc acagaacgtg agcagggtgc ggaagcgctg gccatcgaag
     5941 cgacggagcc tggcgatgct caggctgcga ccgctgcttt caaccagatc gtgaccatcg
     6001 cgcagcaggg gcgtgcgacc attttcagtg agcagcacgc tggcgaacgt tggctcggcg
     6061 cgtggtggga cgatccgata gtcgctgagc aggtagccgt tttcggtgcc gcggtagatc
     6121 gcggagaaga cttctttggc ggttacgctg atgcgtccac cgatgaccag atcgcgctgg
     6181 ccgtctccat ccagatcgtc ggtgtcttcg ccgctgccgt tgtgaatctt gtccgaaccg
     6241 atcgggccgc atggcagcgt cgtcagcttg cacggatttg tgggctcagg ctgcgttgac
     6301 tgcggttcaa tgccacacca cttggcatcg ggcagaacgc catcgctgct ggctgacgcc
     6361 ggggcattgc gggttggcga accgatcggc aagctgggtg cttcggcggt ggcagcctgg
     6421 gaaacggcgg cggcagagcc acccgctggc gcaccgccca gccaggtagg cttctgttgt
     6481 gcactgcgga gcggcggaaa tggtgcattg cgagaaggtt cgtttgcctc cccgacctgc
     6541 acggcccccg ccacaagccc gaagccaagc agggttgcaa gcgccagacg accgacacta
     6601 ctgggcatag tgctcaatat atcaggctct tgcatccgct ttcagtgtgc gtcgggatcg
     6661 gctgtcgcgg ctcgggtttg gttgggacgc ttgcggcgcg ctgcgcagga cgccggccag
     6721 ttgggcatgg gcgatcagct catcggcgtg ggcgcggctc ttttcggtca gtcgggcgcc
     6781 acctatcatt cgggcgattt cgtcgcgtcg ttcggtggga cgcagcggac ggacgctggt
     6841 gacggtgcga ccggacaaga cctgcttttc cacgcggaaa tggtgctgtc cataagcggc
     6901 gatctgggcg aggtggctga tgcacagcac ttgcttgccg tttgccaggc tgcggatctt
     6961 gcaaccgacg cgatcggcgg tggctccacc tatgccggag tcgacttcat cgaagacgca
     7021 ggtggccact tcatcggctt gtccgagcac gccgcgcagc gcaagcatga cccgcgatag
     7081 ctcgccaccc gaggcgatcc gctgcagtgg tcggggatct tcaccgtggt tggcagaaaa
     7141 cagaagctcg cagcgatccc aaccgtcccg ac
//
LOCUS       DAORGN010000234         7131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4863_length_7131_cov_5.730073, whole genome shotgun sequence.
ACCESSION   DAORGN010000234 DAORGN010000000
VERSION     DAORGN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4863_length_7131_cov_5.730073"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2414)
                     /locus_tag="PKL17_18910"
     CDS             complement(<1..2414)
                     /locus_tag="PKL17_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46862.1"
                     /translation="MTAKRYLGLWPALLLMVACHGDRNGSAPTAKPPRPSHTLEALGP
                     SATGQRPRPVANAAAPLRILGYGPQGRADSQAMVFVRFNQPVAPTDRERNPSLVLDPS
                     VPGRTYFASPDLLVFEPSQPLPLARRFSVLLRGRIDSYDGASHSEPLSWSFETAPPSI
                     RFFLLPRRSPDDPQPRTDPVLLAVDQPTTIEELSSHLVVTAVPLLGTVKPSRAVTVQL
                     VRASEADRKRYEVPDKEVASDRLFAAIPLGGWPSGTQVTLTVSKGLRGLQGPLPTDSQ
                     SAVRFQTPQVLSVEQLSCTQKAPCTIEPVVLKFSAPITKQQTRYIKISPLPAAYAPEL
                     VLPDGEGMSQQVLLQGHFRPDAQYRIDIAGELSDRHHQRLGKPVTLRPVFARAPMLRF
                     ASSGGTLPASGRLTVGLESRFVKSVRVRAVLLEPETLVGLSGKNGSDLTWPSRERLQR
                     LSWPDSSTGQTHKQVTLLPSGPTAWASAELDLRELLGDVRGAVLLEAKVTELTPGGGD
                     KLPPPVMILLRVTDLGPVLLTGGNRSLMYVTRLSSGQPIAGAEVHQYAPGSEPLLLGR
                     TDNHGLLSLPGMFEWAQRSPTALLSVSEAASKDRAYLPPDGRVSLIQTNTAPGLTPGE
                     DVRALVLTERRRYGPDDNIRILGMTLLDTAATRTGTRLLPEGTPVQIDLVDEFRQTVT
                     SAEAKTTHEGKFFAELKIPSGTPIGSYAIVAQVLGETAQTRVFIEEPTRPEFSVAAMP
                     IPSDVIAGTRPTVRVTADYSYGGLVRIRKAAYQQRCHAVRFRPPGLPSTWDVGNKPPG
                     DLLDRGT"
     gene            2585..3736
                     /locus_tag="PKL17_18915"
     CDS             2585..3736
                     /locus_tag="PKL17_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK46863.1"
                     /translation="MPYLFETEEHDALRRQVARFAETSIAPHAMAYEEAGQFPRELYR
                     ASAEAGVLGIGFPVEYGGSGGDITHQIVAGEAMILHGRSVGTAVGLGSHAIALPPILH
                     LGSDAQKRHFAPKILHGEWISALAITEPDGGSDVAAIRTRASRQADHYVVQGSKLYIT
                     SGCRADVIVTAVRTDPDLSKRHMGLSLLIVERGSPGFAASRKLHKMGWSASDTAELTF
                     ADCQVPVENRLGDEGSAFFAILRNFAAERLLLASQCVAIAQLAYDESVAFAKLRSTFG
                     KPLARHQVIRHKLADMATQLAAARSLVGECATRCQRGDVVMSQLAMCKNAATDMCSFV
                     VDQAVQIHGGLGYMRETLVERLYRDARLYPIGGGTREIMNEIIAQAEGY"
     gene            complement(3881..5281)
                     /locus_tag="PKL17_18920"
     CDS             complement(3881..5281)
                     /locus_tag="PKL17_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNK46864.1"
                     /translation="MSTPSPACNRALAVFDTKHPQYLEDLCKLVRIPSVSFEGFPAEE
                     VTKSAEATAALLSSRGFENVQVLKLPGVHPYVYGDFLHAPGKPTVLLYAHHDVQPAGD
                     VEAWASPPFQPTERSGRLYGRGTADDKAGIIVHTSALDAYLSAGEKPPINLKVLIEGE
                     EEIGSGNLGAFIDKHLELLQADVMVLTDTGNFDVGVPSITTSLRGLVTVEVEVTSLKQ
                     SVHSGSWGGPLPDAGLALSKILATLADEHGRIAIPGIYDKVRPLSDVEKASLNSLPFT
                     MDQFRNQAGIVECVPTLPACGDNPWTMIWREPSVSVNAVEVSSRRDARNIINGTAWAR
                     VGMRLVPDMDAHEVESALIAHLKAAAPYGVQVNVTPLGAKGPWFTSPEHPAFSAALRS
                     LQAGYGRAPVLIGCGGSIGFVGPLSEKLGGVPALLVGVEDPYTNAHSENESLCIADFY
                     SCIRSQIHLFDQLSRI"
     gene            complement(5323..6399)
                     /locus_tag="PKL17_18925"
     CDS             complement(5323..6399)
                     /locus_tag="PKL17_18925"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46865.1"
                     /translation="MRRHRIVLSVAVQLIFAGCVSSNPDFCTSPDVCGSPDDVRGRDA
                     ATDNLDASMSGGDMATNVGDMSMETALDLSKKGPLSTIRFDLKVNVNRSNVDLTVIGP
                     SSDGKEITKTGAPFPWVLLSPGFSTDHKQYQGYGDRLASYGIVTILQKSPSEWDHTRY
                     RDDTIALISWLLNPMGMGADRLAGRLDKDRLGLTGHSLGGKISFLVAESDSRVKALFG
                     IDPVDTRDPQAAKDIAKIKLPTGVSIGFVGETVSQSGFMPCAPANANYEVLYKAAPAP
                     AFAITMVGAAHMDFVDNPASCWNCGACGGGTAPKDRTNQLTIKYATAYFLSTLGGERR
                     AVDTLSGVQFQKDVTAGFVSRVAK"
     gene            complement(6564..7043)
                     /locus_tag="PKL17_18930"
     CDS             complement(6564..7043)
                     /locus_tag="PKL17_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNK46866.1"
                     /translation="MTFSQAGGGKRAREANRDRKKKEKEERLRRNRAERSSSGRDSSF
                     DIDFASPVETLPEVKLEDIIVSGVAPRGPKGSNGPTKLFVGGLSWDTEGAQLREAFGR
                     FGALKEATVVTDRDSGRSRGFGFVTFENPIDAAAAVREMNGAELDGRTLRVNPADRG"
BASE COUNT         1424 a         2276 c         2081 g         1350 t
ORIGIN      
        1 gttccacgat ccagcagatc gcccggcggc ttgtttccaa catcccaggt cgacggcaag
       61 cccggcggac ggaaccgcac tgcatgacag cgctgctggt aggccgcttt gcgaatccgc
      121 accagcccgc catacgaata atccgccgtc acccggaccg tcggacgggt cccggcaatg
      181 acatctgacg ggatcggcat cgctgcgaca gaaaattccg ggcgcgtcgg ttcttcgata
      241 aacacccgcg tctgtgccgt ttcacccagc acctgcgcga cgatggcata gctaccgatc
      301 ggcgtgccgc tggggatttt cagttcggca aagaacttgc cttcgtgcgt ggtcttcgct
      361 tctgccgacg tgaccgtctg acggaattcg tcaaccagat cgatttgcac cggcgtcccc
      421 tcgggcagaa gccgcgtgcc agtgcgtgtc gccgccgtat ccagcagcgt catgcccaaa
      481 attcggatgt tgtcgtcggg accgtagcgc cgccgttcgg tcagaaccag cgcccgcacg
      541 tcttcgcccg gcgtcaaacc cggtgccgta ttggtctgta tcaatgacac ccggccatcg
      601 ggaggcagat acgcgcggtc tttgctcgcc gcctcgctca ccgacaaaag cgccgttgga
      661 gaacgctgcg cccactcgaa catgcccggc agcgaaagca agccgtgatt gtcggtgcga
      721 cccagcaaga gcggctcact tcccggcgca tactgatgaa cttcggcccc ggcaatcggc
      781 tgtccactcg acagccgcgt cacgtacatc agcgatcgat tgccacccgt cagcagcacc
      841 ggacccaggt ccgtcacccg caaaagaatc atcaccggcg gcggcagctt gtccccaccg
      901 cccggcgtaa gctccgttac tttcgcttcg agcagtactg cgccacgaac atcgccgagc
      961 agctctcgca aatcgagttc cgccgatgcc cacgccgtcg gccccgatgg cagaagcgtc
     1021 acttgtttgt gcgtctgtcc cgtcgatgaa tccggccaac ttaaccgctg gagccgctct
     1081 cggctcggcc acgtcaggtc gctgccattt ttccccgaaa gtcccaccag cgtttccggt
     1141 tcaagcagca ccgcgcgcac ccgaaccgac ttgacgaagc gactttccaa accgaccgtc
     1201 agccgtcccg acgccggcag cgtgccaccg ctgcttgcaa agcgcagcat cggcgcccgc
     1261 gcaaacaccg gacgcagcgt cactggcttg ccgagccgct ggtgatgcct gtccgaaagc
     1321 tccccggcga tgtcgatccg atattgggca tcgggccgga aatggccttg caaaagcacc
     1381 tgctggctca tcccttcccc gtcgggaagc accagttcgg gcgcatacgc tgccggcagc
     1441 ggcgatatct tgatataccg cgtctgttgc ttggtaatcg gcgcactgaa cttgagcacc
     1501 actggctcaa tggtgcacgg tgctttctgg gtacagctta gctgctcgac ggaaagcact
     1561 tgtggcgtct gaaaccgcac ggcgctctgg ctgtccgtgg gaagcggtcc ttgcagtccg
     1621 cgcaggccct tgctcaccgt cagcgtcacc tgggttccac ttggccaccc acccaacgga
     1681 atcgccgcaa acagccgatc cgaggccact tctttgtcgg gaacttcgta gcgcttgcga
     1741 tccgcttcac tggcccgcac aagctgaacc gtcaccgctc gactcggctt caccgttccc
     1801 aaaagcggca ccgcagtgac gaccaaatgg ctgctcagct cctcgatggt cgtcggctgg
     1861 tcgacggcca gtaacaccgg gtcggtgcgc ggttgcggat cgtcggggct tcgccgcggc
     1921 agcaagaaaa agcgaatgct cggtggcgcc gtctcgaaag accagctcag cggctcgctg
     1981 tgcgacgcgc cgtcatatga atcaatccgt ccgcgcagca gcaccgaaaa acgacgcgcc
     2041 aaaggcagtg gctgcgacgg ttcaaacacc agcagatcgg gacttgcgaa gtaggttcga
     2101 ccgggcacac tcggatcgag cacgagcgag ggattgcgtt cgcgatccgt cggtgccacc
     2161 ggctggttga agcggacaaa aaccatggcc tgcgaatcgg cgcgaccctg tgggccgtag
     2221 cccagaattc gcagcggtgc cgcggcgttt gcgactggcc gaggacgctg tcccgtcgct
     2281 gaaggcccaa gcgcttccag cgtatgcgaa ggtcgcggcg gttttgccgt cggtgctgac
     2341 ccatttcggt cgccgtgaca ggcgaccatg agcagcagcg cgggccacag ccccaagtac
     2401 ctcttcgcgg tcacgccaag gtggcgtggc catgcacagc aatcccccaa gccagactat
     2461 tgtatgaaaa tcaaatgtaa ttgtggatac aaaacaatgc agtacaccgg ggccagatgc
     2521 cgatgccacg gccttcggta caccgcgcat gcccgacagc gctgcgagcg cgtaatatcc
     2581 ccatatgccc tacctgtttg aaactgaaga gcacgacgct ctccgacgcc aagtggcccg
     2641 ctttgccgaa acctcgatag cgccacatgc aatggcttac gaagaagccg gacagttccc
     2701 gcgcgaactc taccgagcat ccgcggaggc cggtgtgctg ggaattggtt ttcccgtcga
     2761 atatggcggc agcggtggcg atattaccca tcaaatcgtc gcgggcgagg cgatgatcct
     2821 gcatggccgt tccgtcggta cagcggtcgg gcttggcagt catgcgattg ccctgccgcc
     2881 tatcttgcat ctgggcagcg atgcgcaaaa acgacacttt gcccccaaaa tccttcacgg
     2941 agagtggatt tccgccctgg ccatcaccga acccgatggt ggcagcgatg tcgccgcgat
     3001 acgcacccgc gccagccgcc aagccgacca ctacgtcgta cagggaagca agctctacat
     3061 cacctcgggc tgtcgagccg atgtcatcgt caccgcggtg cgcaccgatc ccgatctgag
     3121 caaacgacac atgggcttgt cgctgctcat tgtcgagcga ggaagccctg gctttgccgc
     3181 gagtcgcaaa ctgcacaaaa tgggctggag cgcatccgat accgccgagc tgacttttgc
     3241 ggactgccaa gttcccgtcg aaaatcggct cggtgatgaa ggcagtgcgt ttttcgccat
     3301 tcttcgcaat ttcgcggcag aacggctgct tttggcctcg cagtgtgtcg cgattgccca
     3361 gcttgcctac gatgaatcgg tcgcgtttgc gaagctgcgt agcacttttg gcaagccgct
     3421 ggcgcgtcat caggtgatcc gccacaagct cgccgacatg gccacgcagc ttgcggcggc
     3481 gcgttcgctc gttggggaat gcgccacacg ctgtcagcgc ggcgatgtcg tgatgagcca
     3541 gcttgcgatg tgcaaaaatg ccgccaccga catgtgcagc ttcgtcgtcg accaggccgt
     3601 acagattcac ggcggactgg gctacatgcg cgaaaccctc gtcgaacgac tttatcgcga
     3661 tgcgcggctg tatccgattg gcggcggcac ccgcgaaatc atgaacgaga tcatcgcgca
     3721 ggccgaaggc tactaacccc cgtcaagtcc cgaacctgca catttcgcgt agggccccgc
     3781 ttcccgacga gactttttcc gtcccgcaag cggtccgaaa aaagcgctcc ggtcgccccc
     3841 acgcttttgc acaaaaatgt tggtttcgta gagtcggggt ttagatgcgg gacagctggt
     3901 cgaacaggtg gatttggctg cgaatgcagc tatagaagtc ggcaatgcac aggctctcgt
     3961 tttccgagtg tgcattggtg tacggatctt cgacaccgac gagcagcgcc ggaactccgc
     4021 ccagtttttc cgacagcgga ccgacgaaac caatcgaacc accacagcca atcaggacgg
     4081 gggcgcggcc atagcccgct tgcagcgaac gcagcgccgc ggaaaacgcc ggatgctccg
     4141 gtgatgtaaa ccacgggcct ttggcgccaa gcggagtgac gttgacctgc acaccatacg
     4201 gcgctgccgc tttcaggtgg gcaatcagcg ctgactccac ttcgtgcgca tccatatcgg
     4261 ggacaagccg cattccgacc cgtgcccacg cggtgccgtt gatgatgttg cgcgcatcgc
     4321 gacggctcga tacttccacg gcattcaccg acaccgacgg ttcgcgccag atcatcgtcc
     4381 agggattgtc gccacaggcc ggcagcgtcg gaacgcactc gacgataccc gcttggttgc
     4441 ggaactggtc catcgtaaat ggcagcgagt tcaggctcgc tttttccaca tccgacaggg
     4501 gccgaacttt gtcgtagatc cccggaatcg caatccgtcc gtgctcatcg gccaacgtcg
     4561 ccaggatctt gctcagcgcc agtccagcat cgggaagcgg accgccccat gaccccgagt
     4621 gcaccgactg tttgagcgaa gtgacttcga cttcgaccgt gaccaggccg cgcaagctcg
     4681 tcgtaatcga cggaactccg acgtcaaaat tgccagtatc ggtcagaacc atcacatcgg
     4741 cttgcagcag ttcgaggtgc ttgtcgataa acgccccgag attgcccgag ccaatttctt
     4801 cctcaccttc gatgagcact ttgaggttga tgggtggctt ttcgcccgcc gacagatacg
     4861 catccagcgc cgaggtgtga acgatgatgc ccgccttgtc gtcggcggtc ccgcggccat
     4921 acaagcgccc gctccgctcg gtcggttgaa acggtggcga cgcccacgcc tcgacatcgc
     4981 cagccggctg cacatcatgg tgcgcataca gcagcacagt cggcttgccc ggcgcgtgca
     5041 aaaaatcgcc atagacgtag ggatgtacgc ccggcagctt cagcacttgc acattttcga
     5101 agcctcggga cgaaagcagc gcagcggtcg cttcggcact tttggtcact tcttcggcgg
     5161 ggaaaccctc gaaactgacc gaaggaatgc gtaccagctt gcacaaatct tcgaggtact
     5221 gcgggtgctt cgtatcaaac acagcaaggg cacggttaca agcaggacta ggcgtagaca
     5281 tggggcttgg attgcgctcc tttcagcgcg aaagcgaaag ggttacttgg cgacgcgaga
     5341 gacgaagccg gcggtgacat ctttttggaa ctgcacgcca gacagggtgt cgacggcacg
     5401 acgctcacca ccgagcgtcg acaggaagta cgccgtggcg tacttaatcg tcagttggtt
     5461 tgtgcgatct ttgggcgcgg tgccgccgcc gcaagcgccg cagttccagc aggacgccgg
     5521 attgtcgacg aagtccatgt gcgcggcccc aaccatcgtg atggcaaacg caggcgcagg
     5581 cgcagctttg tacagcacct catagtttgc attcgctggc gcgcacggca taaagcctga
     5641 ctggctcacc gtttcaccga cgaaaccaat ggaaacgcca gtcgggagct tgatcttggc
     5701 aatgtccttg gccgcttgcg ggtcgcgggt gtcgacggga tcaatgccaa acaaggcttt
     5761 tacccgactg tctgactctg ccaccaggaa gctgatcttg ccgccgagcg agtgcccagt
     5821 caggccgagc cgatctttgt cgagccgccc cgccagacga tcggccccca ttcccatggg
     5881 atttagcagc caagaaatca gcgcgatggt gtcgtcacga tagcgcgtgt gatcccactc
     5941 acttggggat ttttgcaaga tcgtcacgat gccatagctg gccagtcggt cgccgtagcc
     6001 ttggtattgc ttgtggtccg tcgaaaaacc tggcgaaagg agcacccacg ggaacggcgc
     6061 gccggtcttg gtgatctctt tgccatcgct gctcggtccg atcacggtca agtcgacgtt
     6121 cgatcggttg acgttcacct tgaggtcgaa gcgtatcgtc gacagcggcc cttttttgct
     6181 caggtcgagc gcggtttcca tcgacatatc gccgacgttg gtcgccatgt cgccgccgct
     6241 catactggcg tcgaggttgt cggtggctgc atcgcggcca cgaacgtcgt cgggactgcc
     6301 acagacgtca ggcgacgtgc aaaagtcggg gtttgagctc acgcagcctg caaaaatcaa
     6361 ctggacagcg acggaaagga cgatgcgatg gcgtctcata gcgaacgact ccttggcaac
     6421 cagggcagtt tccctacgta aggaacggta ggcctatcta tgtagatgca ggcaagccga
     6481 ataaaagcgc agcaaaccgc cctccgtcaa gaagcggaag gcggacgttt gaacgtctcg
     6541 atggggaaga gaaaaagcgg cgactagccg cgatcggcgg gatttacgcg aagcgtgcgg
     6601 ccatcaagct cggcgccatt catttcacga acagcagccg cagcatcgat gggattttca
     6661 aatgtgacga agccgaagcc gcgcgaccga ccactgtctc gatcggtcac caccgtcgcc
     6721 tctttgagtg caccgaagcg accaaaggcc tcgcggagct gcgcaccctc ggtatcccag
     6781 ctcaagccgc caacgaacag cttggtgggt ccgtttgagc ccttgggacc gcgcggagca
     6841 acacccgaga caatgatgtc ttcgagcttt acttcgggca gcgtctccac aggacttgcg
     6901 aagtcgatat caaaagatga atctcgacca ctgctcgagc gctccgcacg attgcgacgg
     6961 agccgttcct ctttttcttt ctttttccga tcgcgattgg cttcgcgtgc gcgcttgccg
     7021 ccgcctgctt ggctgaaggt catcggggat taccaccgac cgccgccacg accgccgcga
     7081 tcgccacgat cgctccagtc gtcgccgccg ccaccgccga agtcccgatc t
//
LOCUS       DAORGN010000235         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4899_length_7097_cov_5.105226, whole genome shotgun sequence.
ACCESSION   DAORGN010000235 DAORGN010000000
VERSION     DAORGN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4899_length_7097_cov_5.105226"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKL17_18935"
     CDS             complement(<1..869)
                     /locus_tag="PKL17_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46867.1"
                     /translation="MTCCAVKQVSRLDARGVAGPRWFFASVSSLTVALLLSGCSKGGY
                     VGPPGDGPVRPPRDLSTSVTEPDLRNETTDGATDFGAVSDLAGPRDFSVMPDLAIPVD
                     LAVPPPDLTVPPDLTPSGPISGGPCSSGASGATAFRIHFTDSGGRATVSYDVHGLPDK
                     TRWKVGVYGYSVGFTPKFVDPFLGSGGLQLDSSDFIDVELSTAGVPRITSSSLSIWGR
                     SYSTGSSGSFNWQTFTGTGATPLNYVSNVAPYSWYSGDITASIAPSDGGILLRIKAGG
                     SSNSLVVNRLELC"
     gene            1148..1951
                     /locus_tag="PKL17_18940"
     CDS             1148..1951
                     /locus_tag="PKL17_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46868.1"
                     /translation="MHHGMLPASTRRPATHPEGINMARYWYWVIDPAFCDETIIKTFK
                     NYQERRSDMIEIRPGGDLSRVVSGDIVYICAHGHELETYVTSHPNKNGHNTKIVKFDQ
                     LVDMICESRLSTPAPSMKIGIKLLVCWAGGKTLENNQYEMYFKKDTIEKPKYPSLSRF
                     GYNKTKAVEIGFAKPQNALYEWAKQDAITTKEKHDGVLACELAKKLKEKGFNGILVGG
                     FPGRLTAHLRKIKEIDIDVEKIPFSCAISKGTIWFDGDGNTHRSDFIHQ"
     gene            complement(2104..2547)
                     /locus_tag="PKL17_18945"
     CDS             complement(2104..2547)
                     /locus_tag="PKL17_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46869.1"
                     /translation="MTQPTSQTQTTSTPSATDRSVPAESARDESPSPRSSDTTKQLRG
                     FARMNKAKLQRIASEGGIAAQKSRLAHRFTSETASAAGKRGGHRIAQNRDYMKELGRR
                     GGIAKGVSMAQKRAALATVQSGLDAGTLPVDMSVSRTNLASATLA"
     gene            2740..4212
                     /locus_tag="PKL17_18950"
     CDS             2740..4212
                     /locus_tag="PKL17_18950"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNK46870.1"
                     /translation="MQQTEVADDDLSDMVIEHAAPTMPTPESLCDSADTALVQAKLVD
                     ALHELQRLQPNQIKDHERAVRALQETLTIVGDSDESELLGPIDWPKFGERLRLRRTTA
                     GLTQDALADLVGVTGTTIRSLEHNKRRARRGLMLKLLAVPALNLRVSDIELAADRSIG
                     SRWTPTSWLGPAYDPVGMITQLAETLNGPSGQLEQTFAYIESQSARDWMATSASPEYE
                     AAYRGCAPIEPMARRIAEVVGEHALSVSALGSGDGKTEALLVHSLLQQLKLPNKTELF
                     LLDISHSLLNAAHRHIAETLPSLKTYTVHGNFLELPRLPMMVSPQRGRKRLYTMLGYT
                     LVNLNDELRFFRDTLSCCAKGDLFLCDISMAAYGPCDDRKKILRLDPVFQGKMRPSHA
                     TWLGGIIRRNCVGADEVNFRWELTPHASVPGSYGFDAIATVKMRTGGPDRRFLMWRGR
                     RYDPQRLSEALCEIGWNTVERIDYGPGKTKTMSLLLLQKQ"
     gene            complement(4587..5471)
                     /locus_tag="PKL17_18955"
     CDS             complement(4587..5471)
                     /locus_tag="PKL17_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46871.1"
                     /translation="MQTGADGNRQASQGVSLLKTVTVAFLVLGTFSSLYHTYVQQLLF
                     LHHDTGTVSRYAALGRFFGVAFLLLGLSQVYAVLQLARAPVGSSGKGLAGAAAGCLSV
                     NLLFNLVGQVAPDLTLPWFTSTKFFVVLSLLDLAGVLMLLAAVLRVSRRAFDGLAVAF
                     VVLVLTREAIGWGTGLLTSDRSLITMWGGFAVRFVFAAAELGLRYAILQRASQDLASG
                     AGSVPSTEDQPDELLDKSQLRARGLLVGGLWLGGGVLITAIGYAAAAASPTGGRYVVA
                     YGAIIYGVARIIRALNIR"
     gene            complement(5807..6910)
                     /locus_tag="PKL17_18960"
     CDS             complement(5807..6910)
                     /locus_tag="PKL17_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNK46872.1"
                     /translation="MGEIIKRMKGERFIGWYLRWVDSDGRRKQRASGQPTYAEAKRML
                     VEIEARVARGKLGVPERDETTTMTVAELSERYLREYDSPRIRDLPRWVAKQRLILRPV
                     IDAVGAQPAVSFDANQAERLRNRLMRRYAANSTRTQLTSIASVFAWATKNRLLSHNPF
                     VDVKKPKKESRVEFLSSQDARKLLDAADLRAKRDRRGVVLSIATRLGLFGGLRAGEIF
                     GLRWRDCDLARGILTVRKSYGDDTKSGKPRTVPMPDELLDALNRWQPICPPTPEGVVC
                     PLSHDKNAGGGWHKTSRRRPEITNLYRAAGLPVPAAPWHCLRHSYASLFVQSGGSIVT
                     LQKLLGHSDIKTTMIYAHLDDGFVTAEVKKLRI"
BASE COUNT         1478 a         2059 c         2105 g         1455 t
ORIGIN      
        1 atgcagagct cgaggcgatt gacgaccaaa gagtttgagc tgccgccggc cttgatgcga
       61 agcaaaatgc cgccatcgct gggggcaatc gatgcagtga tgtcgccgga ataccagcta
      121 tacggcgcga cgttgctgac atagttcagg ggtgttgcgc cggtgccggt gaaggtctgc
      181 cagttgaagc tgccgctaga tcccgtggag tacgatcgac cccagatgga caggctggaa
      241 ctggtaattc ggggtacgcc agcggtgcta agctcgacgt cgataaagtc gctggaatca
      301 agctgaaggc cgccgctgcc gaggaacgga tcgacaaact ttggggtgaa tccgacgctg
      361 tatccgtaca cgcctacctt ccagcgggtc ttgtcgggta gaccgtggac atcgtaactg
      421 acggttgcgc ggcctccgct gtctgtaaag tgaatgcgaa atgcggtcgc gccgctcgca
      481 ccagacgagc acggtccacc gctaatcggg ccgctcgggg taaggtccgg cggaacggtc
      541 aaatctggcg gtgggacggc caaatccaca gggatcgcaa gatcaggcat gacgctgaaa
      601 tcgcgtggcc ccgccaagtc agaaaccgcg ccaaagtccg ttgcgccatc cgttgtttcg
      661 ttccgcaagt cgggctctgt gaccgacgtg gacaggtcac gcggggggcg cactggtcca
      721 tcgccgggtg ggcccacgta gccccctttc gagcagcccg acagcagcag cgccactgtc
      781 agcgacgaaa ccgacgcaaa aaaccagcgt gggccagcga caccacgcgc atccagtcga
      841 ctcacttgtt tgacagcaca gcacgtcacc acggcatcac cctggcgtct tgtactacct
      901 ctgccgattt ttcactacgg caagacgcaa tttcgtcgat gcggtcgcca cgcttggcct
      961 ggcagcgggg cgatggaaca ggttttccgc cggtgcgcac aacgtgcgct gcctgcgcgc
     1021 acaacctgac gatccgagct gcgtggccga gcctgcatcc cctcatctaa caccagcgtc
     1081 ggaaacagca tccccaagct tgtcgagtgg gtgcttgcgg cgatggtgcc gaccgcctgc
     1141 tgggcccgtg catcacggca tgcttcctgc gtcgacacgc agaccagcaa cgcatcctga
     1201 ggggatcaac atggcacgtt attggtattg ggttatcgac ccggcatttt gcgatgagac
     1261 aatcataaag acattcaaaa attatcagga gagacgttcg gatatgattg aaataagacc
     1321 ggggggagac ttgtctcggg ttgtttcagg agatattgtg tatatttgcg cgcatgggca
     1381 tgaattggag acttacgtta cgtctcatcc gaacaaaaat ggtcataata cgaaaatagt
     1441 taagtttgat cagcttgttg atatgatatg cgaaagcagg ctttcaactc cagcgccgtc
     1501 aatgaaaata gggatcaagc tgctggtttg ttgggctggt ggaaaaacgc tcgaaaataa
     1561 tcagtatgaa atgtatttta aaaaagacac catagagaag cccaagtatc cttctctgag
     1621 tagatttggt tataataaaa ccaaggcagt ggaaattggc ttcgctaaac ctcaaaacgc
     1681 actctatgag tgggccaagc aagacgcgat aacgacaaaa gagaaacacg acggtgtgct
     1741 tgcctgtgaa cttgcgaaga aactgaagga aaaaggtttc aatggaattc ttgttggcgg
     1801 ttttccggga cgattgacag cgcatttgag aaagatcaag gaaatagata tagacgtgga
     1861 aaaaatccct tttagttgtg cgatatcgaa aggaacaatc tggtttgacg gcgacggaaa
     1921 tacgcatcgt tcagatttta tacatcaatg atatctcaaa aaattattga tcgatttcat
     1981 cgcaaaatta tcgatttcgg gactgctttg ttttggcagt ccctgtccgt tgtagatcga
     2041 gcacatttca aaaaattcag cagccttgtt tggttgggtg cagctcgatc catcaattct
     2101 tggctatgca agagtcgcgg atgccagatt ggttcgtgat accgacatat ccactggcag
     2161 cgtaccggca tccaatccgc tttgaacggt cgccaatgcc gctcgtttct gggccatgga
     2221 tacgcctttg gcgattccgc cgcgacggcc cagctctttc atgtagtcgc ggttttgggc
     2281 gattcggtgg ccgccacgct ttccggctgc gctggccgtt tcggatgtga agcggtgcgc
     2341 gagccgtgat ttctgcgcag cgatgccacc ctcgctggcg atgcgctgaa gcttggcttt
     2401 gttcatgcga gcgaagccgc gcagctgctt tgtggtgtcg ctgctgcgcg ggcttggcga
     2461 ttcatcccgt gctgactctg ccggaacact tcggtctgtc gcactgggtg tcgatgtcgt
     2521 ctgggtctgg ctggtcggtt gggtcatggt ggcctccgca atggggtaat tcatggtttg
     2581 tggagcggat tcatctggta gttcgggtgt tcgcgccatg ggtctcttcc gaaccggttt
     2641 ccgaaccaaa ccgaaccagt ggcaagcaga tttcgccgtc gaaattgccc ggtttaccgt
     2701 cgcgtagctg tgctatgtct gcgcccagag gtgacggtga tgcaacagac agaagtcgct
     2761 gacgacgatt tgtcggacat ggtgatcgaa cacgcggcgc cgacgatgcc cactccggag
     2821 tctctttgcg acagtgcgga tacggcgctg gtgcaggcca agctcgtcga cgcgctgcac
     2881 gagcttcagc ggctgcagcc aaaccagata aaagaccacg aacgggcggt gcgggcgctt
     2941 caggaaaccc tcaccatcgt cggggattcc gatgaaagcg agctgctcgg gccgattgac
     3001 tggcccaagt tcggagagcg actgcggctg cgacggacca cggcggggct gacgcaggat
     3061 gcgctggccg acctggtggg ggtgacgggg acgacgatcc gttcgctcga gcacaacaag
     3121 cggcgggcgc gacgcgggct gatgctgaag cttttggcgg ttccggcgct gaacctgcgc
     3181 gtcagtgaca tcgaactggc cgcggatagg agcatcgggt cgcgctggac tccgacgtcg
     3241 tggctcgggc cagcctatga tcccgtcggg atgatcactc agcttgccga gacgctcaat
     3301 ggtccaagtg gccagctgga acagaccttc gcgtacatcg aatcgcagag cgcccgagac
     3361 tggatggcga ccagcgcatc accggagtac gaagcggcct accgtggctg tgcgccgatc
     3421 gaaccgatgg cgcggcgaat tgctgaagtg gtcggtgagc atgcgctcag cgtcagtgcg
     3481 ctcggcagtg gcgatggcaa gaccgaagcg cttctggttc acagtctttt gcagcagctc
     3541 aagctaccga acaaaaccga actgttcctg ctcgacatca gtcactcgct gctcaatgcg
     3601 gcccaccgtc acatcgccga gacgctgccg agcctgaaga cctataccgt gcacggcaac
     3661 tttcttgaac taccgaggct gccgatgatg gtgtcgccgc agcggggccg aaaacggctg
     3721 tacaccatgc tgggctacac cctcgtcaac ttgaacgacg agctgcgatt tttccgcgac
     3781 accttgagct gctgcgccaa gggcgatctg tttttgtgcg acatctccat ggctgcatac
     3841 ggaccgtgtg acgatcggaa aaagatcctc aggctcgatc cggtgttcca agggaaaatg
     3901 cgcccctcgc acgcgacgtg gcttggcggc atcattcggc ggaactgcgt cggtgccgac
     3961 gaggtaaact tccgctggga gctgacgccg cacgccagtg tcccaggcag ctacggattc
     4021 gacgccatcg cgacggtcaa gatgcgaacc ggcgggcccg accgtcgttt tttgatgtgg
     4081 cgcggtcgtc gctatgatcc gcagcgactg tccgaagcgc tttgcgaaat cggctggaac
     4141 accgttgagc gcatcgacta cggccccggc aagaccaaga cgatgtcgct gctgttgctt
     4201 caaaaacagt agtttggggc cgttttcgcc ggcattccgg ctgcgcaagt tcggtttcca
     4261 aaccgggctt cccgatccgc ccacccttgt cgtgcgctaa catgatgaaa cgcttggtac
     4321 ttgcgcgtcg atgcgcttcg acggcagggc tttcggcccg gcgggtttgc catgggctca
     4381 caatcttcca aagacagacc ataccgacgg gcgcttgagc ctgtcgacat cttggacatc
     4441 atgatgccgc gcatgcagtc acagcgacga caacgcacag cgccggatgc agcgtggtcc
     4501 ctgtagggtc cagtccgcag cgtgccgagc tgggatcgtc gctgtctatc gtcagacccg
     4561 atcggcgacc gcccaggtcg gttcggctag cgaatgttta gggcacggat gatgcgtgcg
     4621 acgccgtaga tgatcgcgcc gtaggccacc acgtatcgtc cgcccgtcgg agacgccgcc
     4681 gccgctgcat agccaatcgc cgtgatgagg accccgccgc caagccacaa cccgcccacc
     4741 aaaagaccgc gggcgcgcag ctgacttttg tcgagcagct cgtcgggctg atcttcagtc
     4801 gacggcacag agccggcccc ggacgccagg tcttgactgg ctcgctgcag aatcgcgtat
     4861 cgcagcccga gctctgctgc cgcgaacacg aagcgcaccg caaagccgcc ccacattgtg
     4921 atgagcgagc gatcactcgt cagcagaccg gttccccagc caatcgcttc gcgtgtcagc
     4981 accagcacca cgaatgccac cgccaagcca tcgaacgctc gtcgggaaac ccggagcacc
     5041 gcggccaaca gcatgagcac cccggccaga tcgagcagcg acaaaaccac gaagaacttg
     5101 gtgctggtaa accacggcag tgtcagatcc ggtgcgacct ggccgaccag attgaacagc
     5161 agattgacac tgaggcagcc cgcggcggct ccggcaagac ccttccccga tgaaccgacc
     5221 ggggcgcgcg caagctgcag caccgcgtag acctgcgaca gccccagcag caaaaacgcc
     5281 accccgaaga atcggcccag cgcggcgtag cgtgaaaccg tcccggtgtc atggtgcaaa
     5341 aacaggagct gctgcacata cgtgtgatac aggctcgaaa acgtcccaag cacaaggaac
     5401 gccaccgtca cggtttttag cagcgacaca ccctggctgg cctggcggtt tccgtctgct
     5461 cctgtctgca tcgcccgagt gtaccttttg ggttgcccga ttccaatgac ctgaatcggt
     5521 tgcgcgcagt tcagccctca aacagccatt gcaacaaagc gccatactag acaaatctgt
     5581 ctaatttttg gattgcagtg tgatgcgggc attgggaatc gcatccagat acagaacatg
     5641 aatccgcgca tgctgttcaa agaatgacag aatttccgtc agatatttcc catacgtact
     5701 gctgcgaaag gttctttcct gatcagacag gagtatgtgg tcgtgctgct tgcaaactag
     5761 acaaattggt ctagtttttt ggtcctgcgc atctgggcca tctggatcag atgcgcagct
     5821 ttttgacttc tgcggtgacg aaaccatcgt cgaggtgcgc atagatcatc gtggtcttga
     5881 tgtcgctgtg tcccagcagt ttttgtagcg tgacaatcga gccgccactt tgcacaaaca
     5941 gagacgcata gctgtgacgc agacagtgcc aaggcgctgc cgggaccggc aagccagcgg
     6001 cgcggtacag gtttgtgatc tctggacgac gacgactggt cttgtgccag ccgccaccgg
     6061 cgtttttgtc atggctgagc ggacagacca cgccttcagg ggtcggggga cagatcggtt
     6121 gccagcggtt cagtgcgtcg agcagctcgt cgggcatcgg caccgtgcgc ggctttcctg
     6181 acttggtgtc atcgccataa gacttgcgca ccgtcagaat cccgcgcgcc aggtcgcagt
     6241 cgcgccagcg caggccaaaa atttccccgg cgcgcagtcc gccgaaaagt ccgagccgcg
     6301 tcgcgatcga caggacaacg ccgcgccggt cgcgcttggc tcgcaggtcc gcagcatcga
     6361 gcagcttgcg ggcgtcttgg ctgctcagaa actcgacccg cgactctttc ttcggctttt
     6421 tcacatcgac aaacggattg tgtgaaagca gtcggttttt cgtcgcccag gcaaacacgc
     6481 tcgcaatgct cgtaagctgc gtccgcgtcg agttggcggc atagcgtcgc atcagccgat
     6541 ttcgcagccg ctcggcctgg ttggcatcaa acgacaccgc cggctgggcc ccaaccgcgt
     6601 cgatcacggg ccgcaagatc aggcgctgct tggccaccca gcgcggcagg tcgcgaatcc
     6661 gtggcgagtc gtattcccgc aaatagcgct cgctcagttc cgcgacggtc attgtcgttg
     6721 tttcgtcgcg ttcgggcacg ccgagcttgc ccctcgcgac ccgcgcttca atttcgacga
     6781 gcatgcgctt cgcttcggca taggtcggct gtccgctcgc tcgctgtttg cgcctgccgt
     6841 cgctgtcgac ccagcgcaaa taccagccga tgaaccgttc gcctttcatc ctttttatga
     6901 tctcgcccat gctgtgcgcc ttccgtggtc cccgcacgtc cacgatcagc cctcgctgtg
     6961 ccgccttgcg gtggtgtgag cctttgccta cagcgacggg aatcctttaa attgttacat
     7021 tttttcgtag tcctagatac acggacatca tgatgccgaa gatggatggg atcgaggcgg
     7081 tgcgagcggt ggcggcg
//
LOCUS       DAORGN010000236         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4939_length_7055_cov_6.229286, whole genome shotgun sequence.
ACCESSION   DAORGN010000236 DAORGN010000000
VERSION     DAORGN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4939_length_7055_cov_6.229286"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1300
                     /locus_tag="PKL17_18965"
     CDS             89..1300
                     /locus_tag="PKL17_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46873.1"
                     /translation="MSEGTQTTCRVNVNGTTVEEILDWDYSDDVMSIAEECHFTVDNK
                     ARKYRDLLRIGDKVEFILQNPSVNGGAPTVKHRGRIVRRHPKYNPRDGSTIGITSADL
                     GWHLQNCDAPLWLSLQNKTYADVCDPATSGFFDESWGFTGVRFDGNIRRSLKQGVAVV
                     ALQAQKALDVVHVIQIEPGDKVFDKIVEYSKRINLLVNVSPDGQLCCFLPDYNQEPLY
                     YIRNVDGDESNNVLDCEAEEDAKTRWTEVTVVGEQVGWDGPKDPQNPNASKKRGNVRH
                     PGNLPFLHRHTVADGEMYQNGLAQKHAEWIYKRGRFDAWWVKYTVADHHQNGHWWVAD
                     TMVYVVDDELGLAGNYYVQSVRCRGSKTGGDTTEVVIRLPGVLSASLGVIADPTFRTP
                     ESTTGTPQAAP"
     gene            1297..1827
                     /locus_tag="PKL17_18970"
     CDS             1297..1827
                     /locus_tag="PKL17_18970"
                     /inference="COORDINATES: protein motif:HMM:NF018585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HNK46874.1"
                     /translation="MTFSSIIRKLILRGRTSEGWFSAESWQTAPDEPQPTLSARRAQH
                     YGLTTEPPDGAELVALGVNAGFSNLVSVAEYVTGEPQIEGGEVLLWSKYGHRIKLAKN
                     GDVVVLPAAGRDVVLGDDGSGDIGKIVTVGELRKILQLIKNHVHPETGTTTGQSPTLA
                     TITLAGSPNVKATEPP"
     gene            1849..2202
                     /locus_tag="PKL17_18975"
     CDS             1849..2202
                     /locus_tag="PKL17_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46875.1"
                     /translation="MDVAFSVTRGRLDFARNKDGDFYLDDLAVYAVLATFVAHKTEYA
                     FDGDLGTFRHKITKDGSVTGTRLQGCGDDCLEQCVNDELITSGTCRVDRLRPGALSVT
                     LSWKSPTGKPGKVTL"
     gene            2199..3767
                     /locus_tag="PKL17_18980"
     CDS             2199..3767
                     /locus_tag="PKL17_18980"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNK46876.1"
                     /translation="MSRDFLTETEVLNLAIAYFQVAHTDPVTGRAPQLGPRSFLGQEA
                     RALAQAIGEVLAAAKGIDDDAIPSVYIDASGVTRTRNSKRSLDDWADTLGLPSDVPGK
                     YGRKGATTAKNGGATATGTPGILVATAAQLTDPSGKITLKLRIGFTMPGGGSQAIVID
                     AVTAGSLARLPVGTLLRWTSPPPGLASTLTLTTALSGGGDIETDIELALRLVARLRDR
                     PRGGSAYDWREWTEAAVDSTGSYIGVKRAYVYPVRDGAGSVSIVPLFGGSGRARDPGA
                     AEAAKILAWLNLQKIATDTVYVVRPRFVSGEELTITATVEALPGFGFDWKDTVTTHAI
                     SGTGTSCIVDVAPMSASLMAAVDNGNKPRIAINFPAQAVPFQARVTAYLLDSPVPGLT
                     TLTLDVAMPATPANYRVYPGGGSTIPVAAAIADFVDNLGPSRSSGYGDPRDAWQDDVT
                     VGQLAAVTLAAVDSSSGEKVVRKSPSVGLGVGITIGVGAAAGTGDDFPTFDNVPGQGP
                     QLVELKAIIVLEAP"
     gene            3764..4768
                     /locus_tag="PKL17_18985"
     CDS             3764..4768
                     /locus_tag="PKL17_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46877.1"
                     /translation="MSSEEIAASMIGAFPPGVPELLAPDDKDGGSVTRHLGCIAKTVQ
                     DTAIAAIEELERDITPLTCGSYRLREWEKALGLAYSRTARSGTLAARRAAVISRLREY
                     GPPTKAMLQAVLAPLLDYADPTQLVILECNRAALRTAHTYAGSKTRGGFTYATPAVWD
                     FWVNDADSVAPGCPQADLTLTAADLSKVAAEIETPDGSHFRINFGQLGRGLCIARTIR
                     AYWQGVTTSNVLGRWRLTVWLDGAGLGVLNDATFFCEGFGRRGRSAAQFEWAAVYEPS
                     KSRGAPDFDAAREAVRRVGFATRIGGLVFPADASVGLPAADYGMIPNDNAIPSGFVPG
                     "
     gene            4780..5637
                     /locus_tag="PKL17_18990"
     CDS             4780..5637
                     /locus_tag="PKL17_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46878.1"
                     /translation="MSTFPFVPYATYANNSVPKVTGDALNAEQDLLNALTWPSYLCRP
                     SVKMRCTDGGTIVVQPSPMMIFDAPLNKHTYCAWAETTIDSTSLDVPSGAWPADKWVY
                     VYAYSENRVGKIETSETAPAAIMPAVGVSTASKAFKTGDESRKYLGCFYSDGSSIVRR
                     FRMSDYQYVNLDQVIALSVNTGADPAWSSYVAIDLSDYVGPDAVETTLAARMVGNVNP
                     FDVVLELSPDSGTVPIVACEVEPNTGGAYTAIKRSLVVMPLLSTTIYGRVTPARDPDV
                     RLEVNGWKE"
     gene            complement(6179..6718)
                     /locus_tag="PKL17_18995"
     CDS             complement(6179..6718)
                     /locus_tag="PKL17_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46879.1"
                     /translation="MRSFTLEPLPPDTTPQASRVPRKTWDRAPTPIYMMAKLKSAEIR
                     RVRIGLASGGLGIHRSVSWPKRSQRWTVSHIDSGLSLHSASLLYWEAKRLLDVALLLG
                     LAFGVDFEKDSLAVANARFDAGVLERDWRYYFGEAVHLPREFKAWQIAAKVLRRRNTE
                     QCRSACNPDTSSGERLGLW"
BASE COUNT         1392 a         2178 c         2170 g         1315 t
ORIGIN      
        1 aacccttcct cggccaaatt ctccgtcgga aagggaagat gtgatcaggt gaaagccggt
       61 aatccctccc ccccgtacct atgccagcat gagcgaaggt acgcagacca cttgccgtgt
      121 gaacgtcaac ggcaccaccg tagaagagat ccttgattgg gactactccg atgatgtgat
      181 gagcatcgcg gaggagtgcc attttaccgt cgacaacaag gcgcggaagt atcgcgacct
      241 tctacgcatc ggcgataaag tcgagttcat cctgcaaaat ccgtcagtga atggcggcgc
      301 tccgaccgtc aagcaccgag ggcgcatcgt ccgcaggcat cccaagtaca acccgcgaga
      361 cggtagcacc atcggaatca ccagcgccga tcttggctgg catctgcaaa actgcgacgc
      421 tccgctgtgg ctgtccctac aaaataagac ctacgccgac gtatgcgacc cagcgacgag
      481 cggctttttt gacgaatcct gggggttcac gggggtgagg ttcgacggaa acattcgccg
      541 ctcgctcaag cagggggtag ccgtcgtcgc attgcaggca cagaaggcgc tcgacgtggt
      601 gcatgtgatc caaatcgagc ccggagataa ggtgttcgat aagatcgtgg agtacagcaa
      661 gcggatcaac ctgcttgtca atgtctcacc agacggtcag ctgtgctgct tcctgcctga
      721 ctacaaccaa gagccgctct actacattcg caacgtcgac ggcgacgagt ctaacaacgt
      781 gctcgactgc gaggcagaag aggacgccaa gacacgctgg acggaagtaa cggtggtagg
      841 cgagcaggtc ggatgggatg gccccaagga cccgcagaac ccgaacgcca gcaagaagcg
      901 gggaaacgtc cgccaccctg gcaaccttcc gtttctccat cgtcacaccg tagccgacgg
      961 tgaaatgtac cagaacggct tagcccaaaa gcacgctgag tggatctaca agcgggggcg
     1021 ttttgatgcg tggtgggtta agtacaccgt cgcggatcac catcaaaacg gccattggtg
     1081 ggtcgctgac accatggtct atgtcgtcga cgatgagctt ggcttggctg gcaactacta
     1141 cgtgcagtcg gtgcgatgcc ggggcagcaa gaccggcggc gataccaccg aagtggtcat
     1201 caggctgccg ggcgtgctgt cggcttcgct cggggttatc gccgatccca ccttccgcac
     1261 gccggaatcc acaacgggaa cgccacaggc agcgccatga cgttttcaag catcatcagg
     1321 aagctcatcc tacggggccg cacttcggag ggctggtttt cggcagagag ctggcagacc
     1381 gcaccggacg aaccgcagcc tacgttatcg gctcgtcgag cgcagcacta cgggctgacc
     1441 accgaaccgc cagacggggc cgagctcgtc gcgctggggg tcaacgcagg cttctcaaac
     1501 ctcgtctctg ttgccgagta cgtgaccggc gagccgcaga tcgaaggcgg ggaagtgctg
     1561 ctgtggtcga agtatggcca ccggatcaag ctcgcgaaaa acggcgacgt tgtggtgctg
     1621 cccgcagctg ggcgcgacgt ggttctaggt gacgatggga gcggcgacat cgggaagatc
     1681 gtcaccgtgg gcgagctgcg caagatcttg cagctcatca aaaatcacgt tcacccagag
     1741 acgggaacca cgacgggcca atcacccacg ctagcaacca tcacgcttgc cggttcgccc
     1801 aacgtgaagg cgaccgagcc accataggcc gctcgtacct ttgcccgcat ggatgttgct
     1861 ttttcggtga cacgcgggcg gctcgacttc gcccgaaaca aagacggcga cttctacctc
     1921 gatgatctcg cggtgtatgc ggtgcttgcg acgttcgttg cgcacaaaac cgagtacgcc
     1981 ttcgacggcg acctcggaac ctttcgccac aagatcacca aagacggctc agtgaccggg
     2041 acgcgccttc aagggtgcgg cgacgactgc ctagagcagt gcgtcaacga tgagctcatc
     2101 accagcggaa cgtgtcgggt agaccgtctg cggcccggcg ctctgtccgt cacgctgtcg
     2161 tggaagtcac cgacaggcaa gccggggaag gtgacgctgt gagccgcgac tttctgacgg
     2221 aaacagaagt gctcaacctg gcgatcgcct atttccaggt ggcccacacg gaccccgtca
     2281 ccggacgcgc tccgcagctc ggcccgcgat cgtttctcgg ccaggaagcc cgagcgcttg
     2341 cgcaggcgat tggcgaagta ctcgcagccg cgaaggggat tgacgacgac gcgatcccgt
     2401 ccgtctatat cgacgcctcc ggggtgacga ggacgcgcaa cagcaagcgt agcctcgatg
     2461 attgggccga cacactcggc cttccttcgg atgtccctgg caagtacggt cgcaagggtg
     2521 ccaccaccgc aaagaacggc ggagcaacgg cgacaggcac ccccggaatc ctggtggcca
     2581 ccgctgccca gctcactgac ccaagcggga aaatcacgct gaagctgcgg atcggcttca
     2641 cgatgcccgg cggcggcagt caggccatcg tgattgacgc tgtgaccgct ggcagcttgg
     2701 cgcgcttgcc ggtcgggacg ctgcttcgct ggacttcccc cccgcccggc ctggcttcga
     2761 ccttgacgct taccacggcg ctatctggcg gcggagatat cgagacagat atcgagctcg
     2821 cgctgcgact ggtagcccgg ctgcgcgacc gtccccgagg gggcagcgca tacgattggc
     2881 gcgaatggac cgaagcggcc gtcgactcga ccggctctta cattggcgtc aaacgcgcct
     2941 acgtctaccc ggtgcgggat ggggccggat ctgtctccat cgtgccgctc tttggtggca
     3001 gcggaagagc tcgcgatccc ggcgctgccg aggcagcgaa gatccttgcg tggctcaatc
     3061 tgcaaaagat cgcaacagac acggtgtatg tggtccgccc ccggttcgtg tccggggagg
     3121 agctcaccat cacagcgacc gtcgaggccc tgcccggatt tggcttcgac tggaaagaca
     3181 ccgtaacgac ccatgcgatc agcggcaccg gcacgagctg cattgtcgac gttgccccga
     3241 tgtctgcgtc gcttatggcg gccgtcgaca acggcaacaa gccgcgcatc gcaatcaact
     3301 tcccggcgca ggccgtgccg ttccaagcgc gtgtgactgc ctacctcctc gattcgccag
     3361 taccgggact gaccacgctc acgctcgatg tcgccatgcc cgcgaccccg gctaattatc
     3421 gcgtgtaccc cggcggaggc agcacgatcc cggtcgctgc tgcgatcgcc gacttcgtcg
     3481 acaatctcgg cccgtcgcgg tcaagtggct acggcgaccc ccgcgacgct tggcaagacg
     3541 acgtgacggt gggccagctc gcggcggtaa cgcttgctgc ggtcgactca tcgagcggcg
     3601 aaaaggtcgt gcgcaagtct ccctcggttg ggcttggcgt cggtatcacg atcggtgtcg
     3661 gcgcggccgc tggcaccggt gacgacttcc cgacgttcga caatgtcccc ggacagggac
     3721 cgcagctcgt cgagctgaag gcgatcattg ttctggaggc tccatgagtt cggaggagat
     3781 cgccgcatcg atgatcggcg ccttcccgcc gggcgttccc gagctccttg cgcctgacga
     3841 caaggacggt ggatcggtaa ctcgtcacct ggggtgcatc gcaaagacgg tgcaagacac
     3901 cgcgatcgcc gccatcgagg aactcgagcg cgacatcacc ccgctaacct gcggctccta
     3961 ccggctgcgg gagtgggaaa aagcgctcgg ccttgcctac agccgcaccg cccgcagcgg
     4021 aacgcttgca gctcggcgcg ctgcggtgat ctcgcggctg cgggagtacg ggccgcctac
     4081 gaaggcgatg ctgcaagccg tgctcgctcc gctgcttgac tacgccgacc cgacacagct
     4141 tgtgattttg gagtgcaacc gagccgcgct tcgcactgcg cacacctacg ccgggtcgaa
     4201 aacgcgcggt gggttcacct acgccacccc ggccgtgtgg gatttttggg tcaacgatgc
     4261 ggactcggtc gcgccgggat gcccacaggc agacctcacg cttaccgcag ctgacctgtc
     4321 caaagtcgca gcggagatcg agacaccgga tggaagccac ttccggatca acttcggcca
     4381 gctcggccgg ggcctgtgca ttgctcgcac catccgcgcc tattggcagg gggtgacgac
     4441 cagcaacgtg ctcggccggt ggcggctcac ggtttggctc gatggagcgg ggcttggtgt
     4501 gctcaatgat gcgacgttct tttgcgaggg gttcggccgc cgtgggcggt ccgctgcgca
     4561 gtttgaatgg gcggcagttt acgagccgtc gaagtcgcga ggggccccgg acttcgacgc
     4621 agcccgcgag gcagtgcgcc gcgtggggtt cgcaacccgg atcggtgggc tcgtgttccc
     4681 cgctgacgcc tcggtgggat tgcctgctgc tgactacggc atgatcccaa atgacaacgc
     4741 cataccatcg ggattcgttc ccggataagg actctgcaag tgtctacctt tccatttgtc
     4801 ccgtatgcga cctatgcaaa caacagcgtc cccaaggtca caggcgatgc gctcaacgcc
     4861 gagcaagatc tgctaaatgc gctgacgtgg ccaagctact tgtgcaggcc atccgtcaag
     4921 atgcgatgca cggacggcgg cacaatcgta gttcagccgt caccgatgat gatctttgat
     4981 gctccgctca acaagcacac ctactgcgcc tgggccgaga caacgatcga ctccacaagt
     5041 ctggatgttc cgtccggggc ttggcctgct gataagtggg tctatgtcta tgcctacagc
     5101 gaaaaccgcg tgggcaaaat cgagaccagc gaaaccgcgc cagctgccat catgcccgcc
     5161 gtcggagtct cgacagcgtc caaggcattc aaaaccggcg acgagtcccg gaagtatctg
     5221 ggatgcttct acagcgacgg ttcctccatc gttcgccggt ttcgcatgtc tgactatcaa
     5281 tacgtcaatc tcgatcaggt gattgccctg tcggtcaaca ctggcgcaga cccagcatgg
     5341 tcaagctatg ttgctatcga cctaagcgac tatgtcggcc ctgatgcggt agaaaccacg
     5401 cttgccgcgc gcatggtcgg caacgtcaac ccgtttgacg tggtgcttga gctgtcgccc
     5461 gattctggga cggttccgat tgtcgcctgc gaagtcgagc caaatacagg cggagcgtat
     5521 acggcgataa agaggtcgct cgtcgtgatg ccgcttttga gcacgacgat ttacggcagg
     5581 gtcacgccag cccgagatcc cgacgttcgc ctcgaagtca acgggtggaa ggagtagccc
     5641 ggtggaagct gatagcttta ttggattggt cgccaagtgg cttttcggca ttgacccggc
     5701 cgtcgtcagc tggaaacacg ttgcgctagc cctggtaatg gtttcgccag cgtgggtcac
     5761 ggggcttatt cgccttgtgt ggtatcttgt gcatatgcgt tttttgcgct cggctacccc
     5821 tcagcagctg gacgcctacc gaagcaacca gccgcgtccg ccaacgattg ttggcccgtt
     5881 catcgtgctt tggttcctgc ttgggatggg attcttgacg gcatcgagcc aggccggaca
     5941 gatggcagag tcggacgcat cgtcggccaa atcgccttgc cctggtggat gcccagctgg
     6001 cagcacttgc aaccccaaga cgagggcatg cgaagccacc gctgcaaagc caggtaacgg
     6061 cgctgcaaga tcgcgcaaca tggaatcgac catcaccaaa gccgcaccgc tcggtgccga
     6121 tctggcccac gatcgatact atgaagtcaa cgccaacccg gctgactact tcgacagctc
     6181 accagagtcc taacctttcc ccgctcgatg tgtctggatt gcaagcagat cggcactgtt
     6241 ccgtgttgcg cctgcgtagc accttggctg cgatctgcca ggctttgaac tcgcggggaa
     6301 ggtgcaccgc ttcgccgaag tagtagcgcc aatcgcgttc gagtactccg gcatcaaatc
     6361 tagcgttagc aacggcgaga gagtcttttt caaagtccac gccaaacgcc aggccaagca
     6421 gcagcgcaac gtcaagcagg cgcttggcct cccaatacag caggctggcg ctgtgaaggc
     6481 tcaagccgct gtcgatgtgc gagacagtcc agcgctgcga acgctttggc caagagaccg
     6541 atcgatgtat gcccagaccg ccggacgcta ggccgatgcg cacacgtcga atttcggcac
     6601 ttttgagctt tgccatcatg tagatcggag ttggcgcacg gtcccacgtt ttgcgaggga
     6661 cgcgcgaagc ttgcggggtc gtgtccggcg gcaggggttc gagtgtaaaa gagcgcatgt
     6721 ttgcgggctc ctagtccggc aggtcgacgg gctgacactt tgcgagctct ttgctcagct
     6781 cctttttgat ggcttcggct tcggcttggc tcgaagttac cgagaaccta gcggaagctg
     6841 gcgaatcgtc gtcatcgaag gcggccgcag aacttctaag tcagcatgcc acgttgcgac
     6901 agctgcaaaa ccacccgctg cgcgactagc tcggccaata tttcgagctc tgccggactc
     6961 atgcgtggct cacttgaggg caacaaacgc ggcccgcatt gcgttctcgg gccagtgttt
     7021 gccgcgctga aggttcttta gctgcgggta ctggc
//
LOCUS       DAORGN010000237         7048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4948_length_7048_cov_5.537395, whole genome shotgun sequence.
ACCESSION   DAORGN010000237 DAORGN010000000
VERSION     DAORGN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4948_length_7048_cov_5.537395"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1610)
                     /gene="tssC"
                     /locus_tag="PKL17_19000"
     CDS             complement(132..1610)
                     /gene="tssC"
                     /locus_tag="PKL17_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNK46880.1"
                     /translation="MTAPTEHAAPSASVLDQIIEQTHAAKAEASRPLMQRGVRALLKQ
                     ILAAQGDTKSAEATKIDRSAVDRAIAELDKKLGQQLDKVLHNEKFQQLESGWRGLRFL
                     VDRTDFRQNIKVSVLNVSKDDLLADFKDAPELFQSGLYYHVHKAEYGQFGGKPYGAII
                     GNYDFGPSEPDIELLRFVGGVASTAHAPFIAAADPRFFGIGSYEGLPRLKDVRSQFES
                     KQYISWRSLRDSEDARYIGLTCPRFLLRRPYGIDNPVKSFGYTEESGSHDHFLWGNTA
                     FAFATRLTESFAKYRWCPNIIGPQGGGTVEQLPVHLYKSDGDLVQKIPTEVLIPEVLE
                     YDLSEEGFIALTMRKDSDNACFFSANSIQKPKTFGQSPEGKAAETNSRLGAQLPYMFI
                     VSRLAHYIKVLQREALGTDKEKKDLQNDLEKWIRQYVTEDDNPMPGIRAQRPLRMADI
                     KVEDVAGRPGWYSVALKVRPHFKYMGASFTLSLVGQQGPPGK"
     gene            complement(1749..2255)
                     /gene="tssB"
                     /locus_tag="PKL17_19005"
     CDS             complement(1749..2255)
                     /gene="tssB"
                     /locus_tag="PKL17_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNK46881.1"
                     /translation="MAATPGKEGAVAPQERVNIRYKTETGGAVEETELPLRLLVTGDF
                     TGRPDATLIEERKPISIDKNNFDEVLSSQRVGLAMQVDNTLSGKPDEQLAVHLNFSSM
                     KDFEPESIAQQIPELRELLELREALTELKGPLSQNAEFRKRLQKLLDDEAERGSILDE
                     IGLGSSNS"
     gene            complement(2390..4030)
                     /locus_tag="PKL17_19010"
     CDS             complement(2390..4030)
                     /locus_tag="PKL17_19010"
                     /inference="COORDINATES: protein motif:HMM:NF028299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HNK46882.1"
                     /translation="MIETIQACSLSQIGKSAAQSGEVLDGNPTLQQFTQIVSDYSGNK
                     LGIDFASVERLGTELAARLPDLKIYSYLSLAVFRNADGEPTRYIKLGALLLAFLDLLA
                     SPDTVQKLFPKNDSRRLGQLQWMSSELALFFEMSPPSPSEAAEFLACKQIAEQVAEQA
                     GTAFGLQHPLLRELREILKKQAETLPPPVEKKAIPIEPKGVEKPAEPEPVKPVPIVRE
                     VVPQQVIVNPPELPPSPPPLQDVATLEIDEVEAHLADCVIRFSDHLRSESIENPAPYW
                     MLRALRFGSHDLLRPERLAQAEANKGKTDLPVPQGHARIKKELTQRLAAGHYAEVAKE
                     CEELFAQHPLWLDLQRFSGEALAQMGASRALSAVLAQAALLVTLCPELPALRFSDRDA
                     TPLADSETVAWLEKAARQKSGAETGHAVGKEAEDAAETGLPDELLAAAKVLQKRIAQA
                     GSGRQRFSIRLQLAEMLLAKQRSDIAMPVIEQLLADIETHRLSDWQPELAQKSLRLAV
                     RVARAAEVDPQTRRALWNRICQIAPADAIELGPEIWPA"
     gene            4197..4955
                     /locus_tag="PKL17_19015"
     CDS             4197..4955
                     /locus_tag="PKL17_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK46883.1"
                     /translation="MNEITLSPLLAKAQTGSLVIETGSLAGQRFVLPPVPGALLLGRE
                     RICNVRFDPERERLVGRTHARIEVRPEGVFLVDLNSANGTFREGGQPVRGSIPLHSGM
                     RFQLGGEGGPWLSVELPQAVPTQAVHASPVLSETPTIVTRPDPIAQHAAPAVLIRPVV
                     KSPVLPASPATAPAQPNIFSASPPSPQVDVDSPRAVSAGSLGQDPQAAAQKRAYFRQI
                     VVIVLILLSACGLGVLIGTSSSSTNLGPSENSVP"
     gene            complement(5096..5854)
                     /locus_tag="PKL17_19020"
     CDS             complement(5096..5854)
                     /locus_tag="PKL17_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK46884.1"
                     /translation="MRTVSRLITLGFLLLSFAVSSCNDTSQTGTMDMNTPPPDQSTGL
                     SVAAISPAGGINTGGTTITITGQGFQSGATVTVFGAPCQNVTVQSPTSLTCTTPAKAG
                     TCGAAAIVVTNPDGKSVSRSDLFAYTSATLSFSPMNTISTGSSPRFVAIDDWNGDGKV
                     DLAVGLIAGSGQVNIHNGDGQGSFGAGNGITAGMQPRDIVSKDLNGDGKPDLIVANQM
                     SNNITVHLASGTSFGAPTTLTPGMGPQGLALADA"
     gene            6007..>7048
                     /locus_tag="PKL17_19025"
     CDS             6007..>7048
                     /locus_tag="PKL17_19025"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNK46885.1"
                     /translation="MTTLASSIRPRWDLSRRTAAFPFLAALKQRVLVCDGAMGTMLQR
                     AGLTVDDFAGKDGCNEILCETRPKAVADVHAAYFAAGADIVETNSFGSTSLVLAEYGI
                     ADRAYDLSRLSAQIARQVADSYSTTDWPRFVAGSVGPTTKLVTLGHVSWDDLFASYAT
                     QMRGLIDGGADFLLIETAQDLLSVKCAVLAARRAMEESGREVPIFTQLTIETTGTMLV
                     GTDVTAALPALEAIEPDGIGLNCATGPDLMQEHVRFFGQNASRFVSVQPNAGLPRNEG
                     GVATYDLKPEMLADFQERFVREFGVSLVGGCCGTTPAHIAAIARTVADLAPRPVPEAA
                     PAMRLSGLEPFTI"
BASE COUNT         1294 a         2097 c         2085 g         1572 t
ORIGIN      
        1 cctcgatctt gccttgctcg gttccctcga cggtcatatg cgcttgcagt gccataggct
       61 gtagctcctt tttccaaatg gagtgtttca agtgaagccg cgacagaacg gctctgccgc
      121 agcctgcgcg attattttcc tggcggtccc tgctgaccca ccagcgatag cgtgaacgac
      181 gcgcccatgt acttaaagtg cggccttacc ttgagcgcga cggagtacca gcccggacga
      241 cccgcgacgt cttccacttt gatgtcggcc atccgcagtg gacgctgggc acgaatgcct
      301 ggcatcggat tgtcgtcttc ggtgacgtac tggcgaatcc acttttcgag atcgttttgg
      361 aggtcttttt tctctttgtc ggtcccgagc gcttcccgct ggagcacttt gatgtagtgc
      421 gcaagtcgcg agacaatgaa catgtatggc aactgcgcgc cgaggcgaga attggtttcc
      481 gcagctttcc cttccggact ctgtccaaag gtctttggct tttggatcga gttggcagaa
      541 aagaagcagg cgttgtcgct gtccttgcgc atcgtgagcg caatgaagcc ctcttccgaa
      601 agatcgtatt ccagcacttc cggaatcagc acttcggtcg gaatcttctg caccaaatcg
      661 ccatcggatt tgtacaggtg aaccggcagt tgctcgacgg taccgccgcc ctgtggaccg
      721 atgatgttcg gacaccacct gtacttggca aagctctctg tgagtcgcgt tgcaaacgca
      781 aacgccgtgt tgccccacag gaagtgatcg tgactgccgc tctcttcggt gtagccaaac
      841 gacttgaccg gattgtcgat tccatacgga cgacgcagca aaaagcgcgg gcaggtcaga
      901 ccgatgtagc gggcatcttc ggaatcacgc aggctccgcc acgaaatgta ctgtttgctt
      961 tcgaactggc tgcggacatc tttgagccgg ggcagtcctt cgtagctgcc gatgccaaaa
     1021 aagcgcggat cggcagcggc gataaacggc gcatgggcgg ttgatgccac accaccaacg
     1081 aagcgcagaa gctcaatgtc gggttcgctt gggccaaagt cgtagttgcc aatgatggcc
     1141 ccatacggct tgccaccaaa ctgaccgtac tccgctttgt gaacgtggta gtagagcccc
     1201 gactgaaaca gctcgggcgc gtctttgaag tccgcgagca ggtcgtcttt gctgacgttg
     1261 agaacgctga ccttgatgtt ctggcggaag tcggttcggt cgacgagaaa gcgcagtcct
     1321 cgccaccccg attcgagctg ctgaaacttt tcgttgtgca gaactttgtc gagctgttga
     1381 cccagttttt tgtccagctc ggcaatcgcg cgatcgacgg cagaacgatc gatctttgtc
     1441 gcttccgccg acttggtgtc cccttgcgct gccagaattt gcttgagcag tgcgcggaca
     1501 cctcgctgca tcagcgggcg actggcttct gctttcgccg catgggtctg ttcgatgatc
     1561 tgatcgagga cactggccga cggtgcggcg tgttcggttg gggccgttat ttgtgccgac
     1621 tgggcagcag tagactcttg ggccacgggc tctccttgcc accttgggaa caccgcaatg
     1681 agcggatgta ttcccaatcc atcttgcggg ttacgaaacc gatgcgtgtg atcctgacat
     1741 ccgagcgatc aggaattgct gcttcccaaa ccgatctcat ccaaaatgct gccgcgctcg
     1801 gcttcgtcgt ccagcagctt ctgaagtcgc ttgcgaaact cggcgttttg cgacagcgga
     1861 cctttcagtt cggtgagcgc ctcgcgcagc tccaaaagct cgcgcagctc cggaatctgc
     1921 tgggcaatcg actccggttc aaagtccttc atgctcgaaa agttcaagtg gaccgcgagc
     1981 tgctcgtctg gttttcccga cagtgtgttg tcgacttgca tcgccagacc gactcgctga
     2041 ctgctcagca cttcatcgaa gttgtttttg tcgatgctga tcggtttgcg ttcttcgatg
     2101 agcgtggcat ctggacgccc ggtgaagtcc cccgtcacaa gcaggcggag cggcagctct
     2161 gtttcctcaa cggcgcctcc cgtttcggtc ttgtaacgga tgttgacacg ctcttggggc
     2221 gcgacggcgc cctctttccc tggagttgct gccatgatcg tgagcctcca caaaaacaat
     2281 cggcaccgct gccgaatgag gtctgcgtag caaaagcaag gcggatttgc aaggctatct
     2341 gctcaacgtg gcttgcgtgt ccgacggcag attgcgcagt ttggatgcat tacgcgggcc
     2401 agatttccgg gcccagttcg atcgcatctg cgggcgcgat ttggcagatc cgattccaca
     2461 gcgcccgtcg cgtctgcgga tcgacttcgg cagcgcgcgc gacccgaacc gcaaggcgca
     2521 gcgacttttg cgcaagctct ggctgccaat cgctgagccg atgcgtctcg atgtcggcca
     2581 gcagctgctc gatcactggc atcgcgatgt cactccgctg cttcgcaagc aacatctccg
     2641 caagctgaag ccgaatggaa aaacgctgtc tgcctgagcc cgcttgcgca atccgtttct
     2701 gcaacacctt tgcggcggca agcagttcat ccggcagacc ggtttccgcc gcgtcttctg
     2761 cttcttttcc gacagcatgc ccggtttccg ctccgctttt ctgacgggcc gctttttcca
     2821 gccacgccac cgtttcggaa tccgcgagcg gtgtcgcatc gcgatcggaa aaacgcagag
     2881 ctggcagttc cggacacagc gtgacgagca gcgctgcttg cgcgagcacg gcggaaagcg
     2941 ctcgcgaggc ccccatctgt gcaagtgctt cgccggaaaa ccgctgcaaa tcaagccaca
     3001 gcggatgttg cgcaaacagc tcttcacatt cctttgcgac ctcggcgtaa tgtccggctg
     3061 cgagtcgctg ggtcagttcc tttttgatcc gcgcatgtcc ttgcggaacc ggcagatcgg
     3121 tctttccctt gttcgcttcc gcttgcgcaa gacgctcggg acgcagcagg tcatggctgc
     3181 caaaacgcag cgcccgcagc atccaatacg gagccggatt ttcgatcgat tcactgcgca
     3241 agtgatcgga aaaacggatc acgcaatcgg cgagatgcgc ttccacttcg tcgatttcca
     3301 gcgtcgccac atcttgcaga ggtggtggcg atggcggaag ctcgggcgga ttcacgatca
     3361 cttgctgcgg caccacttcg cgcacgatcg gaacgggttt gacaggttct ggttcggctg
     3421 gcttttcgac ccctttgggt tcgatgggaa tcgctttttt ctcgacggga ggtggcagcg
     3481 tctctgcttg ttttttcaga atctcccgca gctcgcgcag aagcggatgc tgtagtccaa
     3541 acgctgtgcc ggcctgctcg gcgacttgtt ccgcgatctg tttgcacgcc aaaaattccg
     3601 ccgcttcgct cggcgaaggc ggtgacattt cgaaaaacag cgcaagctca ctggacatcc
     3661 actgtagctg tcctagccgt cgagaatcat tcttcggaaa cagcttttgg acggtgtccg
     3721 gactcgccag cagatccaaa aacgccaaaa gcagcgcacc cagcttgatg tagcgcgtcg
     3781 gctctccgtc tgcattgcgg aacaccgcca gcgacagata gctgtaaatt ttcagatcag
     3841 gaagccgcgc cgccagctct gtgcccagcc gctcgacgct tgcaaagtca attcccagct
     3901 tgttgccgct gtaatcagac acaatctggg taaactgctg gagagtcgga tttccgtcga
     3961 gaacttcgcc agattgcgca gccgacttcc cgatctgcga aagcgaacaa gcttggatcg
     4021 tttcaatcat ccgcgggtct cccgattgtg tgcgatgcgt cgcgtatagc atgggcagac
     4081 atccgctcct agcgttcccg cttgccaatc cggaagagtc tgtcttacgc tccgccgcgt
     4141 gacccgttgg caaagcgccg tgggccacaa aacctgcgct cggcttgggt cttcctatga
     4201 atgagatcac gctctctccg ctgctcgcca aggcacagac cggaagtctg gtcatcgaaa
     4261 ctgggagtct ggccggacag cgttttgtgc ttccgcctgt gcccggtgcg ctgctgttgg
     4321 gtcgcgagcg catctgcaat gtccgttttg atccagagcg cgagcggctg gtcggacgca
     4381 cgcatgcgcg aatcgaagtc cgtcccgaag gcgtgttcct ggtcgatctc aacagtgcca
     4441 acggaacgtt tcgcgaaggc ggacagcctg tccgtgggag cattccgctt cattccggaa
     4501 tgcgtttcca gctcggcggg gaagggggtc catggctgtc tgtggaactg ccccaagcgg
     4561 tgccgactca ggccgtgcat gcatcgccgg ttctatcgga aaccccgacg attgtgacgc
     4621 ggcctgatcc catcgcccag catgcagcgc cggcagtgct gattcgtccc gtggtgaaaa
     4681 gtcctgtcct gcccgcttcc ccggcgaccg cgcccgcgca accgaacatt ttttctgcga
     4741 gcccacccag tccgcaagtc gatgtcgaca gtccgcgcgc cgtaagtgct ggcagcctgg
     4801 gtcaagaccc ccaagcggcg gcgcaaaaga gagcgtattt ccgacagatt gtggtcattg
     4861 tgctgattct gctttcggcc tgtgggctcg gtgttctcat cggaacgagc agcagttcca
     4921 caaaccttgg cccctctgaa aattccgttc cgtaatctgt gtcgtggatc ggtgcggatg
     4981 acccgccacg tcgattccct gcgtagatgt cctgaccatt ccttccctgt ccgtacctat
     5041 cctgaccgaa ccgttcatcg cccctgtcat caagtggcgc caatccaaaa ttcagttaag
     5101 cgtccgccag tgccaggcct tgggggccca ttccgggggt tagggtggtc ggcgcgccga
     5161 agctggttcc gctcgccaag tggacggtga tgttgttgct catttggttg gcaacgatca
     5221 ggtcgggctt gccatcgccg tttagatcct tggacacgat gtcgcgcggc tgcatgccgg
     5281 cggtgattcc attgccggcg ccaaagctgc cttgtccgtc gccgttgtgg atgttgactt
     5341 gaccgctgcc ggcgatgagc ccgaccgcca gatcgacttt cccatcgcca ttccagtcat
     5401 cgatcgcgac aaagcgtgga gatgatccgg tgctgatggt gttcatcggt gaaaacgaca
     5461 gcgtcgcact ggtataggca aacagatcgg aacgcgaaac gctcttgccg tcgggattgg
     5521 tgacgacgat ggcggcggct ccgcaggttc cggctttggc cggggtggtg caggtcaggc
     5581 tggtcgggct ctgtacggtg acgttttggc agggcgcgcc aaagacagtg acggtggcgc
     5641 cgctttggaa gccttgtccc gtgatggtga tggtcgttcc gccggtgttt atgccgcccg
     5701 cgggtgaaat ggccgcgacg ctcagcccgg tgctctgatc gggcggcggt gtgttcatgt
     5761 ccatggtgcc ggtttggctt gtgtcattgc agcttgaaac cgcgaagctg agaagcaaaa
     5821 agcccaaagt gataagccga gagacagttc gcatgaaggt tccccccttg acgttgtgcg
     5881 agtcgatgcg tgccgacgat ggggatgctt ttgcaaaaag aagcgacgcc cccaaagggc
     5941 acacaatcat ccaaagtagt cgattgcgac aaaccctgac aatgtaccgt gataggttgc
     6001 tgcgaaatga ctaccttagc ttcgtcgata cgtcctcgct gggatctttc tcgtcgcact
     6061 gcggcgttcc cctttttggc agctctaaaa cagcgtgtgc tggtctgcga cggagcgatg
     6121 ggaaccatgc tccagcgcgc cgggctcacc gtggacgact ttgctggcaa agacggctgc
     6181 aacgaaatcc tgtgtgaaac ccggcccaaa gccgttgcgg atgtgcatgc agcgtacttt
     6241 gcggcgggcg cggacatcgt cgaaaccaac agctttggct cgacgtcgct ggtgctcgcg
     6301 gaatacggca tcgccgatcg cgcttacgat ttgtcgcggc tttcggcgca gattgctcgc
     6361 caggttgcgg acagctactc gacgacggat tggccgcggt ttgtcgccgg ttccgtcggt
     6421 cccacgacca aattggtgac gcttggccat gtgagctggg acgatctgtt tgcctcttac
     6481 gcgacccaga tgcgcggact catcgacggt ggagccgact ttctgctgat cgaaacggcg
     6541 caggatctgc tcagcgtcaa gtgtgcggtg ctggcggctc ggcgcgcgat ggaagaatcg
     6601 ggtcgagaag ttcccatttt cacccagctt acgattgaga cgactggcac gatgctggtc
     6661 ggtacggacg tcacggcggc gctgccggcg ctggaagcga tcgaaccaga cggcattggc
     6721 ctaaactgcg cgacggggcc ggacctgatg caagagcacg tccgcttctt tggccaaaac
     6781 gcgagccgtt ttgtatcggt acagcccaac gccggactgc cgcgcaacga aggcggcgtc
     6841 gcgacgtatg acctaaagcc cgagatgctg gccgactttc aggaacgctt tgtccgagag
     6901 tttggcgtgt ctttggtcgg tggctgctgc ggcaccacgc ccgcgcacat cgcggccatc
     6961 gcacgcacgg tcgccgacct cgctccgcgc ccggtgcccg aggccgcccc ggccatgcgg
     7021 ctgtccggtc tcgagccgtt cacgatcg
//
LOCUS       DAORGN010000238         7045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_4954_length_7045_cov_6.843920, whole genome shotgun sequence.
ACCESSION   DAORGN010000238 DAORGN010000000
VERSION     DAORGN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4954_length_7045_cov_6.843920"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="PKL17_19030"
     CDS             complement(<1..1802)
                     /locus_tag="PKL17_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNK46886.1"
                     /translation="MAITRRVHWLCAAGFLALFQDQSAHAQNRDNFRNPTFDMQLYRP
                     AIDSRGYVTVNSSKLLGHLDFSLGLQGTWAFQPLYLQLNGGSNYAGDPSAPAVTNTRT
                     FSVDHLVTPQLQFAIGLFKWVELGIGLPLGLAIGKRQLCDANGENCGGYNQAASSDQL
                     RYTKVMLSDIGLHSKIRVLDPSSFPIGLAALLSVYFPISKWANDDGYKTFLGEDNVTL
                     RPQLIIEREWGSSRRIRTAINVGALVRFGSTAFTDIGKSIAVGNTQTCYPSDATMPTG
                     ECNPGTTFGGTGLSRSVGTQVTYGISAAFGVVQNRFDILVEAYGYADVTGNDKAFPVE
                     AVGAIKLYLARNSFFLAGAGAGVYGNGGEQTGSPRARAFLGFVYEPRIGDRDGDGIRD
                     NIDQCPDEPEDLDGFQDEDGCPELDNDNDGIPDSRDQCPNVPGTWDNKGCPTDSDRDG
                     DGIPDSKDKCPDDPEDRDGWEDDDGCPELDNDGDGIPDHLDKCPNEAETVNGIDDKDG
                     CPDGEDTDKDGIPDSQDKCPYEPEDKDGFEDSDGCPELDNDKDRIPDKDDKCPNEPET
                     YNGFDDTDGCPDKGRVMMEGGKINILDKVYFETG"
     gene            2079..2975
                     /locus_tag="PKL17_19035"
     CDS             2079..2975
                     /locus_tag="PKL17_19035"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNK46887.1"
                     /translation="MWKLAVALLVLGNLDQGIFADLSGRVAIHSPNWSVSDGPLAIRI
                     DDQHRILTVWRGSVALVAYPIIATPTTLSGLSRTPSRDDLLLLIDKADSEEAKAQLGA
                     PFSVKWGPPPKYEDRDGDGIVNTLDVLIGAKKLCENKAAYVSNYRRLSYPGGDVPRTE
                     GVCTDTLVRALRNAGWDLQSGIHEDALRKPKLYPLEKPPDANIDHRRIRMMLPYFRQF
                     FVELKKDEPYLPGDLVLFDTFPSKAGPDHAGIVSDRLGPSGLPLVVNNWTDGYVEAEM
                     DLLPTVPITHRFRVPMTPRATP"
     gene            3072..4547
                     /locus_tag="PKL17_19040"
     CDS             3072..4547
                     /locus_tag="PKL17_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNK46888.1"
                     /translation="MHLLWLAILSTVVMLAMGCGSATMVIDCVLTPDICGPGGECDAE
                     TRQCRRKAGSPCVSSEQCAQDDRPVCNGAICVACDVLSTPESADQACQQKNQKGVAAC
                     LRAGIRKGSCGECRLDAHCGDGSRPICDPSTFTCRACQKHSECTDSQVCNLGGGLVDL
                     PDIKVGQCVPVSKVVFVDADRCPRSGSGPTGSADKPYCEISSALGKADFVVVRPRTSG
                     LYGGLSVSDGKRTTIVGPGRDAMTPVQVAALSVSGSQTALVVSDITVRGDSVGASCGK
                     DGNLWVLRSIVQNGAVGIEALGCNRLVIEQSIVSGNRSLGLRIGTGTRQYRIVNNVFS
                     GNGQSVGGFGVFVAAGVSGLLSFNTILNNGEKGVDGGAIRCETGGMTTRAVANSIIIQ
                     NAMSARTDGSGQPLGTQFSGDCRLNRIVVGIDGTVVVPPSEGLIRAIPDLDQSMRLLS
                     TPNNRMYVIDKGVADDSVRNDHFGSPRPKGDGYDLGAHELR"
     gene            4680..4985
                     /locus_tag="PKL17_19045"
     CDS             4680..4985
                     /locus_tag="PKL17_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNK46889.1"
                     /translation="MPTYDYECQNCGHTFETSQRITEKALTDCPECKQPKLQRLITAA
                     AFHLKGGGWYKDGYGSGGSARTENQRIDKLQSKINESKASESSSDSSSSGSSGDSSS"
     gene            5056..5346
                     /gene="groES"
                     /locus_tag="PKL17_19050"
     CDS             5056..5346
                     /gene="groES"
                     /locus_tag="PKL17_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNK46890.1"
                     /translation="MKVRPLHDRVLLRRKTEEERTAGGLYIPDTAKEKATQAIVVAVG
                     KGRVLDDGSIAAPKVKAGDTVLFGKYAGTEIKLDGEEHLIMREEDLIAVIDS"
     gene            5386..>7045
                     /gene="groL"
                     /locus_tag="PKL17_19055"
     CDS             5386..>7045
                     /gene="groL"
                     /locus_tag="PKL17_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831647.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNK46891.1"
                     /translation="MAAKDILYSQHARNEIARGLNALANVVKVTLGPRGRNVVIEKSW
                     GSPTVTKDGVTVAKEVELEDKFANMGAQMVKEVASKTSDIAGDGTTTATVLGQAIYTE
                     GAKMVVAGHNPMEIKRGIDEAVAVVIGELRTMSKPTKGHKEIAQVGTISANGDAVIGN
                     LIADAMEKVGKEGVITVEEAKSMETTLNVVEGMQFDRGYISPYFVTDSERMEAVLEDP
                     YILISEKKISNMKDLIPTLEQIARSGKPLLIIAEDIDGEALATLVVNKLRGTLHCCAI
                     KAPGFGDRRKEMLKDIAILTGGQAITEDLGLKLENLTIKDLGRAKRVKVDKDNSTIVD
                     GAGKKADIDARVKQIRAQVADTSSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRTLPKLAALKLPGDRQYGVAIIRRAIEEPL
                     RWIAQNGGVEGTVVVDKVKNSQGGFGYNAATDKYEDLIEAGVIDPTKVVRTALQNASS
                     VAGLMLTTETLIAEKPKKKEKAAAGGGGMGGMGGMGGMGGMGGMG"
BASE COUNT         1443 a         2046 c         2193 g         1363 t
ORIGIN      
        1 ttgcccgtct cgaagtacac cttgtcgagg atgttgatct tgccgccttc catcatgacg
       61 cggcctttgt cggggcagcc gtcggtgtcg tcaaagccgt tgtaggtctc cggctcgttt
      121 ggacacttgt cgtccttgtc gggaatgcgg tctttgtcgt tgtcgagttc cgggcagccg
      181 tcgctgtcct cgaaaccgtc tttgtcttct ggttcgtagg ggcacttgtc ttggctgtcg
      241 gggatgccgt ctttgtcggt gtcttcaccg tcagggcagc cgtctttgtc gtcgatgccg
      301 ttgaccgttt cggcttcgtt tggacacttg tccaggtgat cgggaatgcc gtcgccgtcg
      361 ttgtcgagtt ccgggcagcc gtcgtcatct tcccagccat cgcggtcttc gggatcgtcg
      421 ggacacttgt ctttgctgtc gggaatgccg tcgccgtcac gatccgagtc cgtcggacag
      481 cctttgttgt cccaggtgcc gggcacattg gggcactggt cgcggctgtc gggaatgccg
      541 tcgttgtcgt tgtctagctc cgggcagccg tcttcatcct ggaatccgtc gaggtcttcc
      601 ggttcatccg ggcactggtc gatgttgtcg cggatgccgt cgccgtcgcg atcgccgatg
      661 cgcggttcgt atacgaagcc aaggaaggcg cgggctctcg gactgccggt ttgctcgccg
      721 ccgttgccat agacaccggc cccagccccg gccaaaaaga acgagttgcg tgccagatac
      781 agcttgattg cgcccaccgc ttcgacggga aatgctttgt cgttgccggt gacatcggcg
      841 taaccatacg cctcgacgag aatgtcaaag cgattttgca ccacgccgaa tgccgccgat
      901 atgccgtagg tcacttgggt tccgacggac cgacttaggc cagtgccgcc aaacgtcgtg
      961 ccggggttgc attccccggt cggcatcgtc gcgtcggatg gatagcaagt ttgggtattt
     1021 ccgacggcga tgctcttgcc gatatcggta aatgccgtcg agccaaagcg aaccagggct
     1081 ccgacgttga ttgcggtacg aatgcgccga gacgaacccc actcgcgctc gatgataagt
     1141 tgcgggcgga gcgtgacgtt gtcttcgcca agaaacgtct tgtagccgtc gtcgtttgcc
     1201 cacttcgaga tcgggaaata caccgacaga agcgccgcca ggccgatcgg gaagctcgac
     1261 ggatcgagga cgcggatttt gctgtgcagc ccgatgtccg acagcatcac tttggtgtag
     1321 cggagctgat cgctcgatgc ggcctggttg tagccaccgc agttttcacc gtttgcgtcg
     1381 cacagctggc gtttgccgat cgccagaccc agcggcagac cgatcccaag ttccacccac
     1441 ttgaacagac cgatcgcaaa ctgaagctgc ggtgtgacca ggtgatcgac ggaaaacgtc
     1501 cgcgtattgg tcaccgccgg tgcacttggg tcgcctgcgt aattcgatcc gccatttaac
     1561 tggagataca gcggctgaaa cgcccacgtc ccttgcagcc cgagcgaaaa atcgaggtgc
     1621 ccgagcaact tcgacgagtt caccgtcacg tagccacgcg agtcgatcgc tggccgatac
     1681 agctgcatgt cgaaggttgg gttgcggaag ttgtcgcggt tctgggcgtg cgcagactga
     1741 tcctgaaaca gagccaaaaa acctgcggca cagagccaat ggactcgacg agtgatcgcc
     1801 acgatgctgc ctcccctttg gcgcatcctc tgtggataga ggtgagttga ccaatgattt
     1861 tacaaaaatc ggtgaaatga aaaccggaaa acaaggcgaa agcttgtcac ttgcgctgcg
     1921 ctttcgtcgc caagcccagc cgcaaatcgc tgtgtggttt gccgtcgatg tttggtcgtg
     1981 gtgtcgggtg gggtacgacg cttgcgaaac ctgcgcctcg ggtacattga attggatcgg
     2041 tttgcatagg tgccgcgttt tggcccgcgg aggattcgat gtggaagctg gccgtcgctc
     2101 tgcttgtgct cgggaatctc gatcaaggca tttttgccga cctttctgga cgggttgcga
     2161 tccattcgcc caattggtcg gtttcagatg gaccgcttgc cattcgcatc gatgaccagc
     2221 atcgaatttt gacggtgtgg cgcggctcgg tggcgcttgt tgcgtatccg atcattgcga
     2281 cgccgacgac gctttcgggt ctgtcgcgca cgccgagccg ggacgatctg ctgctgctta
     2341 tcgacaaagc ggacagcgag gaagccaaag cccagcttgg cgcgccattt tccgtaaagt
     2401 ggggcccgcc gccgaagtac gaggaccgcg atggcgatgg gatcgtcaac acgctcgacg
     2461 tgctcatcgg cgccaagaag ctctgtgaaa acaaagccgc atacgtctca aactatcggc
     2521 ggctaagcta ccccggcggt gacgtgccgc gcaccgaggg cgtctgcacc gatacgctgg
     2581 tgcgggcact gcgcaacgcc ggctgggact tgcaaagcgg tatccacgaa gacgcgctgc
     2641 gcaagccgaa gctgtatccg ctggaaaagc cgcccgacgc caacatcgac catcggcgga
     2701 ttcgcatgat gctgccgtac tttcgtcagt tcttcgtcga gctgaaaaaa gatgaaccgt
     2761 atctgccggg cgatctggtg ctgttcgata cgttcccgag caaagccggt cccgatcacg
     2821 ccggcatcgt gtcggatcgg ctgggtccat cggggctgcc gctggtggta aacaactgga
     2881 ccgacggtta tgtcgaagca gagatggatc ttttgccgac ggtgccgatt acccaccgat
     2941 ttcgtgtgcc gatgacaccg cgggccacgc cgtaacgtgc gaccgattgc tgtcccgtcg
     3001 gctgcttact tgacaccacc tgcccgcgct gcctacgctc cttaccctgc tcgccgggac
     3061 tgtgcgaaaa gatgcacttg ctgtggctgg ccatattgtc gacggtggtg atgctcgcga
     3121 tgggctgcgg ctcggcgacg atggtcatcg actgcgtgct gacccctgac atttgcggtc
     3181 ccggtggtga gtgcgatgcc gaaacccgcc agtgccgtcg caaagccggt tcaccgtgtg
     3241 tcagctctga gcagtgcgcc caagacgatc gtcccgtctg caacggcgcc atctgcgttg
     3301 cctgcgacgt tctttccacc cccgagtcag ccgatcaggc ctgccagcaa aagaaccaaa
     3361 aaggcgtcgc ggcttgtctg cgcgccggga ttcgcaaagg aagctgcggc gagtgccgac
     3421 tggatgccca ctgcggagat ggcagtcgac ccatctgtga tccgtcgacg ttcacctgtc
     3481 gcgcctgtca aaaacacagc gagtgcaccg attcgcaggt ctgtaacttg ggcggcggac
     3541 tcgtcgactt gcccgatatc aaggttgggc agtgcgttcc ggtgagcaaa gtggtctttg
     3601 tcgatgccga tcgctgtcca cgcagcggat ctggtccgac gggcagcgct gacaagccat
     3661 attgcgaaat atcgtctgcg ctgggcaaag ccgactttgt ggtcgtccgt ccgcgcacca
     3721 gcgggctgta tggcgggctc tcggtcagcg atggaaaacg taccaccatc gtcggtcctg
     3781 gccgcgatgc gatgacaccc gtgcaagtgg ccgcgctcag cgtgagtggc agccagaccg
     3841 cgctcgtggt cagcgacatc acggtgcgtg gtgacagcgt cggtgcaagc tgcggcaaag
     3901 acggcaacct gtgggtgctg cgcagcatcg tccaaaacgg tgcggtgggc atcgaagcgc
     3961 tgggctgcaa tcgcttggtc atcgagcagt cgatcgtgag cggcaaccgc agcctgggtc
     4021 tacgaatcgg caccggcacc cggcaatacc gcatcgtgaa caacgtcttt agcggcaacg
     4081 gccaatcggt cggcggcttt ggtgtgttcg tcgctgctgg cgtcagcgga ctattaagct
     4141 tcaacaccat cctcaacaac ggggaaaagg gcgtcgacgg tggtgcgatt cgctgcgaga
     4201 ccggcggaat gaccacgcgc gccgtcgcaa attccatcat catccaaaac gcgatgagcg
     4261 ctcgcaccga tggcagcggt cagccacttg gaacgcagtt ttcgggcgac tgtcgattga
     4321 atcgaatcgt cgtcggcatc gacggcacag tcgttgttcc gccgagcgag ggcctgattc
     4381 gcgcgatacc cgacctcgat cagagcatgc ggcttttgag cacgcccaac aaccgcatgt
     4441 acgtgatcga caaaggagtg gcggacgaca gcgtgcgaaa cgatcacttc ggttcgccca
     4501 gacccaaggg tgacggctac gatctgggcg cccatgagct gcgctgaact gtcaaagaag
     4561 cagccaaagt ccttgaaaat caacgacttt gcagaattcg ttagcactcg tggggggtga
     4621 gtgcttgaca atctgtagcg cgtacttagc gtatcagcgc cgtttggaag ggttgcacca
     4681 tgccgactta cgactacgag tgccaaaact gcggtcacac gtttgaaacc tcccagcgca
     4741 ttaccgaaaa agcgctcact gattgcccgg agtgcaagca gccgaagctc cagcgactga
     4801 tcacggctgc ggccttccac ctcaaagggg gtggttggta caaggacggc tacggaagtg
     4861 gcggttctgc ccggaccgaa aatcagcgca tcgacaagct gcaatcaaaa atcaacgaat
     4921 cgaaagcgag cgagtcctcg tcggacagct cgtcttcagg cagctccggc gactcgtcga
     4981 gctaacgcct acgattcgtt cccctaacga ctcaatcgca gactgaaaaa ccaaaacacc
     5041 ccaaggagga tttcgatgaa ggttcgtccc ctacacgacc gtgttctgct caggcgcaag
     5101 accgaagaag agcgcaccgc tggtggtctg tatatccctg atactgcaaa ggaaaaggcg
     5161 acccaggcca tcgtggtggc ggttggcaaa ggccgcgttc tcgatgacgg aagcattgcg
     5221 gcgcccaagg tcaaagcggg cgacaccgtg ctgtttggca agtacgccgg caccgagatc
     5281 aagctcgatg gtgaggagca cctcatcatg cgcgaagagg atctcatcgc ggtgatcgac
     5341 agctagtcga aaatcccccc tgtttggcca aaaggaacca gcaacatggc agccaaggac
     5401 attctttaca gccagcacgc tcgcaatgag atcgcccgcg gtctcaacgc gcttgccaac
     5461 gtcgtcaaag tgacgctcgg tccgcgtggc cgcaacgtcg tgatcgaaaa gtcgtggggc
     5521 tcaccgacgg tgaccaaaga cggcgtcacc gtggccaagg aagtcgaact tgaggacaag
     5581 ttcgcgaaca tgggcgccca gatggtaaaa gaggtcgcga gcaagacctc tgacatcgca
     5641 ggcgacggca ccacgacggc gacggtgctc ggccaggcca tctacaccga aggtgccaag
     5701 atggtcgtcg ccggtcacaa cccgatggaa atcaagcgcg gtatcgacga ggccgtcgcg
     5761 gtcgtcattg gcgagctgcg cacgatgtcg aagccgacga agggtcacaa agagatcgcc
     5821 caagtcggaa cgatcagcgc caacggcgac gccgtgatcg gcaacctgat cgccgacgcg
     5881 atggaaaaag tcggcaagga aggcgtgatc accgtcgaag aagccaagtc gatggagacc
     5941 acgctgaacg tcgtcgaggg tatgcagttc gaccgtggct acatctcgcc gtactttgtc
     6001 accgacagcg agcggatgga agccgtcctc gaagatccgt acatcctcat ctccgagaag
     6061 aaaatctcga acatgaagga tctgatcccg acgctggagc agattgctcg ctcgggcaag
     6121 ccgctgctca tcatcgccga ggacatcgac ggtgaagcgc tggcgacgct ggtcgtcaac
     6181 aagctgcgtg gcacgctgca ctgctgcgcc atcaaggccc caggctttgg cgaccgtcgc
     6241 aaggaaatgc tcaaggacat tgcgatcctg accggtggcc aggcgatcac cgaggatctc
     6301 ggcttgaagc tggaaaacct gacgatcaag gatctgggcc gcgccaagcg ggtcaaggtc
     6361 gacaaggaca actcgacgat cgtcgacggc gctggcaaaa aagcggacat cgacgcgcgc
     6421 gtcaagcaga tccgcgctca ggtggccgac accagcagcg attacgatcg tgaaaaactg
     6481 caagagcggc tggcgaagct cgtcggtggc gttgcggtga tcaaggttgg cgcggcgacc
     6541 gagaccgaga tgaaagagaa gaaggctcgc gtcgaggatg cactgcatgc aacccgcgcc
     6601 gccgtggaag aaggcattgt ccccggtggt ggtgttgcgc tgatccggac gctgccgaag
     6661 ctcgccgcgc tgaagcttcc gggcgaccgc caatatggtg tggcgatcat ccgtcgcgcc
     6721 atcgaggagc cactgcgctg gattgcccag aacggcggcg tcgaaggcac tgtggtcgtc
     6781 gacaaggtca agaacagcca gggtggcttt ggctacaacg ctgcgaccga caagtacgaa
     6841 gacctgatcg aagcgggcgt catcgatccc accaaggtcg tgcgcaccgc tctccagaac
     6901 gcttcgtcgg tggcaggact catgctgacc acggagacgc tcatcgctga aaagcccaag
     6961 aagaaggaaa aggccgcagc cggtggcggc ggaatgggtg gcatgggcgg aatgggtggc
     7021 atgggcggaa tgggtggcat gggcg
//
LOCUS       DAORGN010000239         6954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5057_length_6954_cov_6.105378, whole genome shotgun sequence.
ACCESSION   DAORGN010000239 DAORGN010000000
VERSION     DAORGN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_6954_cov_6.105378"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2816
                     /locus_tag="PKL17_19060"
     CDS             <1..2816
                     /locus_tag="PKL17_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46892.1"
                     /translation="LIEARAAARLQHPNVVTIYRVGEIDAHPYIIAEYIQGQDLQALP
                     KPVPWQFALELAIGLSRGLAAAHKRGVLHRDVKPANAILADNGEVKLLDFGLAKYIDK
                     PDAVSVRWANQEVVAPTMEATASGHFSPLSADATPPFDGPVKISVQGQAALSAAQLVP
                     LPAAFPAVAQSDDQIRPVELRKTDPPKTPLGYSRSSQIKGTPIYMAPERLLGAPATRR
                     SDVYSMGALFYELLSGVPPHFDVPFEKLPQVVPERDVPPLTHLAPSVDPQLSQVVDRC
                     LKRNPADRYASGEDLQAALEQCVKGKHAAMLPEGNPYRGVLAYDSDHRSLFFGRRADC
                     GTLLDRLRSEPVMLVAGDGGSGKTSLVRAGLIPLIGEGAIGGGRRWRTISTQPGRHPL
                     QSLSEALAETIGPRSEQSDLRAMMETDPQALQKLLQKRTSERRGIALFIDQLEDLLHH
                     GSPAEAKLYLQTLATLFEPHPGLRLVLAAQSQSIGPLSSLSPDLLDWTRDVYFLKPLP
                     RDRLREVIVEPAQSKGIRFEPATFVDELLDFAQQSDGGLSLLGIVLAELWDSRHDNAM
                     GLQALSAIGGLAGAIARHADHVIGSLSRDRRIVARRLLLLLVTPEKALCRRSEDELSS
                     HSPDAQRVLHVLLSGRLVCMTETPSGGSYQLAHPLLATSWPTMQRWIDEQAPVQPSRP
                     SLPAIRIATEPPVYKHFRRIGWSLLPTALLVLGLSGSAYYEQIQFKRKNLTLLASAQQ
                     AIAEGQSRQALVTQYQKLALEMLDGPRRDKVQEQWELARSAAAQAERAYSRASQALEQ
                     SLRGSENQRAVRDDLARLLYDRALIAEWAHEEQLLDELIKRIQLYDDKGTWMVRWQRP
                     GRLSLSSQPTGAKVTVQRYVIDAQGKWFVTENTELGTTPLRGHVLSPGSYLITVSLAG
                     QRDIRLPTVVGHDEEIKQHVTLP"
     gene            2800..6558
                     /locus_tag="PKL17_19065"
     CDS             2800..6558
                     /locus_tag="PKL17_19065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNK46893.1"
                     /translation="MSRSPEIDWNPPKLFDGYRVLWTISRTEHSSLCMGQDTVLDRPV
                     QIRFLDVAEHVKDQRDRFVAEARAVAKVQHPNVMTVHRVGTIDSRPYIISEYVRGQTV
                     EQLPKPLPWRQVLPIATAAARGLAAAHRRGVLHRSLRPSNILVASDGAIKLTEFGLRE
                     FCGEEISQTRAISDGTIQPADSQPELLLPQSAEELRRKAEQFVSPEVKAKEPPTQRAD
                     LFALGMILYELILGKLPSPAILAQPPADDLQAAGWPLDIDARFAKIVLRCLRSRPQER
                     FGSSEELESSLEQLLPRGGVDKIPEGNPYRGLLPFEAEHRSLFFGRRSEIGTLVERLR
                     TDACVLVAAESGVGKSSLCRAGVLPLVAEGALAGGRAWRVVSMVPGRHPLRALAQGLS
                     SVCQQSDDDFVRDISAEPERLGRILHQRLGNDRGLLLFVDQLEELSTIADPREAQIVG
                     EALGSLLARITSVRLLITVRSDYLGRVASLPSIGEAVTRSLYILRPLGPDRIREAVVG
                     PAHVKGVLFESTDLISGLVDSTASTDGALPLLQFTLAELWEAREGNRITQAALDNIGG
                     VTGALSRHADHVIGTLPPAQRQSARRVLMSLVTLEGTRARRTEEELINSDSNARSALE
                     ALVRGRLLVARDTADGTAYEVAHEALIKGWITLSRWLEEFAESRAVKQRLETAATEWR
                     RMSRSPDALWSAAQLAELALVESADIAAKEAEFVAASWRNVRKRTLLRNLLIGSLPLL
                     AVLSYSAFEYTAHRNLSIRIDGYLRDGKSDLDAARRRNREVDELRRAAFSAFDSMQNN
                     DGEEMWNRALALSLDAERAYSRAGQTFEAALTADSTNIPARELLADVLYERALIAERD
                     RRPQQLDDLLQRMALYDLSGARKLKWNAPSTISVRKSPDSAQVSIARYERDPKGRRIL
                     TTPRDLGAAPTISTTMPPGSYLLTLSAPGFADVRYPVFLTRSETVELSIDLPAQARVP
                     DGFVYVPPGRFLFGSGSEETMRKSMLTAVPIHQVQTGGFLIAKHETTFADWLTFLSAL
                     SPTERSRYLSKPTPSSLPGAVGLTQLPGGEWELTIQPTSKKYILRENQKLVYDTRKIH
                     REHDWHKLPVSGISFQDAKDYAAWLAKSGKLPGARLCSELEWERAAKGADDREWPHGD
                     DILAGDANFDATYNFDAVSMGPDEVGSYPLSKSPFGINDMAGNAFEWTISSLAKDEAS
                     VRSSSYFFAAIVQRSTNRNTLDPSMRSPGVSMRLCATLSLAKPEPARN"
     gene            6746..>6954
                     /locus_tag="PKL17_19070"
     CDS             6746..>6954
                     /locus_tag="PKL17_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46894.1"
                     /translation="MRLQRVVSFLGSGLLFLASGCGHEDGPALTLDLNRLDSMQSNDD
                     VVNGLTLNGLTLNGLTLNGLTLNGLT"
BASE COUNT         1382 a         2204 c         2073 g         1295 t
ORIGIN      
        1 atctgatcga agcgcgcgcc gccgcccggt tgcaacatcc gaacgtcgtc accatttatc
       61 gcgttggtga aatcgacgcc cacccgtaca tcatcgccga gtacatccaa ggccaggatc
      121 tgcaagcact gcccaagccg gtgccctggc agtttgcgct ggaattggcc attggactat
      181 cgcgaggtct ggctgcagcg cacaagcggg gggttttgca ccgcgacgtg aagccagcga
      241 acgccatttt ggccgacaac ggagaggtga agctgctcga ctttgggctg gcgaagtaca
      301 tcgacaagcc tgatgcggtg tcggttcgct gggccaatca agaagttgtc gcaccgacga
      361 tggaagcgac ggcgtccggg catttttcgc cgctttccgc cgatgccacg ccgccgttcg
      421 atggcccggt gaaaatctcg gtgcagggac aggcggcgct gtccgcagcc cagcttgtgc
      481 cgctgccggc agcattccca gcggtggcgc agtcggatga tcagattcgc cccgtcgagt
      541 tgcgcaaaac cgatccaccg aaaacaccgc tcggatattc gcgcagcagc cagatcaaag
      601 gaacgccgat ttacatggca ccggagcgac tgctcggagc tcccgcgacg agacggtcgg
      661 atgtctattc gatgggcgca ctgttttacg aactgctgtc gggtgtgccg ccgcatttcg
      721 atgtcccgtt tgaaaaactg ccgcaggtgg ttcccgaacg tgatgtgccg ccgctgaccc
      781 atctggcccc gtcggtagac ccacagcttt cgcaggttgt cgaccgctgt ctgaagcgca
      841 atcccgccga ccgctatgca tcgggggaag acttgcaggc ggcgcttgag cagtgtgtca
      901 aaggcaagca tgccgcgatg ctgcccgagg gaaacccgta tcgcggcgtg ctcgcgtatg
      961 acagcgatca tcggtcgctt ttcttcggtc gacgagccga ctgcggaacg ctgctcgacc
     1021 ggctgcgcag tgaaccagtg atgctcgtcg ctggggatgg tggaagcggc aagacgtcac
     1081 tggtgcgcgc cggacttatc ccactgattg gggaaggagc catcggcggc ggtcgtcgct
     1141 ggcgcaccat ttcgacccag ccgggacgac atccgctgca gtcgctgagc gaagcgctgg
     1201 ccgaaacgat cgggccacgc agcgagcaga gcgacctgcg ggcgatgatg gaaaccgatc
     1261 cgcaagcgct gcaaaaactg ctgcaaaaac gaacgagtga gcgacgcggc atcgccctgt
     1321 tcatcgatca gcttgaggat ctgctgcacc acggctcgcc cgccgaagcc aagctgtatc
     1381 tgcagaccct ggcgacgctg tttgaaccgc acccagggct gcggcttgtg ctggcggcgc
     1441 agagtcagag catcggccct ttgtcatcgc tgtcgcccga cctgctcgat tggacgcgcg
     1501 atgtctattt tttgaaaccg ctgccgcggg accgtttgcg agaagtcatc gtcgagcccg
     1561 cgcagagcaa aggcatccgc ttcgagccag ccacatttgt cgacgagctg ctcgattttg
     1621 cccagcagag cgacggtggg ctgtcgctgc tcggaatcgt gctcgccgag ctgtgggatt
     1681 ctcgtcacga taacgcgatg ggacttcagg cgctgtcggc gattgggggt ctggccggtg
     1741 cgattgcccg gcacgccgat cacgtcattg ggtcgctgtc gcgcgatcgc cgcatcgtgg
     1801 cgcgacggct gcttctgttg ctggtcacac cagaaaaagc gctgtgcaga cgcagtgaag
     1861 acgaactgtc atcacacagt cccgacgcac agcgagtact gcacgttctt ttgagtggcc
     1921 ggctggtctg tatgaccgaa accccgtcgg gtggatcgta ccagctggcg catccgctgc
     1981 tcgcgacgag ctggccgacg atgcagcgct ggatcgatga acaagcgccg gttcagccgt
     2041 cacgaccgtc gctgccggcg atccggattg ccaccgagcc gccggtttat aagcactttc
     2101 gccggatcgg gtggtcactt ttgccgacgg cactgctggt gctcggactc tccggctctg
     2161 cgtattacga acagattcag ttcaagcgca aaaacctgac gctgctggcc agtgcccagc
     2221 aagcgattgc ggaaggacag agtcgacaag cgctggtgac gcagtatcaa aagcttgcgc
     2281 tcgaaatgct cgacggtccg cgacgcgaca aagtgcaaga gcagtgggaa ctggcgcgat
     2341 cagcagcggc gcaagccgaa cgagcctaca gccgagccag tcaagcgctc gaacagtcgc
     2401 tgcgcggcag tgaaaaccag cgggccgtcc gcgacgatct ggcgcgcctt ttgtacgacc
     2461 gcgcgctgat tgccgagtgg gcgcacgaag aacagcttct cgacgagctt atcaagcgca
     2521 ttcagctata cgacgacaaa ggtacgtgga tggtgcgctg gcaacgtcca ggccgcctgt
     2581 cgctgagcag tcagccaacc ggggccaaag tgaccgtcca gcgctacgtt atcgacgccc
     2641 aaggcaagtg gtttgtcacc gaaaacaccg agctagggac cacaccgctc cgaggacacg
     2701 tactcagccc agggagctac ctcatcacgg tctctctggc cggacaaaga gacattcgtc
     2761 tgccaaccgt cgttggccat gacgaggaga tcaagcagca tgtcacgctc ccctgaaatt
     2821 gactggaacc caccgaagct gttcgacggc tatcgtgtcc tgtggacgat cagccgaacc
     2881 gaacacagct ctttgtgcat gggacaagac accgtgcttg atcgtccggt tcagatccgc
     2941 ttcctcgacg tagccgagca cgtcaaggat cagcgtgacc gcttcgtcgc agaagcccgc
     3001 gcggtggcca aagtccagca tcccaacgtc atgaccgtcc accgcgtcgg caccatcgac
     3061 agcagaccgt acatcatcag cgagtacgtc cgcggccaaa ccgtcgaaca gcttcccaag
     3121 ccgctgccct ggcgacaagt gctgccaatt gccaccgcag cggcacgcgg gttggcggca
     3181 gcgcaccgac gaggtgtttt gcaccgcagc ttgcgtccga gcaacatctt ggtggcgtcc
     3241 gacggcgcga tcaagctgac agagtttgga ctgcgtgagt tctgcggcga agaaatctcg
     3301 caaacccgcg cgatttcaga cgggacgatt caaccagctg actcccagcc ggaactgctg
     3361 ctgccgcaga gcgcagagga actccgacgg aaggccgagc agtttgtgtc ccccgaagtc
     3421 aaagccaaag aaccgccgac ccagcgcgcc gatctgtttg cactcggaat gatcctgtat
     3481 gagctgattt tggggaaact gccgtcgcca gcgatcttgg cacagccacc tgccgacgac
     3541 ttgcaagcgg cgggttggcc gctggacatc gacgcgcgct ttgccaaaat cgtcttgcgc
     3601 tgtctgcgat cccgtccgca agaacggttc ggctcgtcgg aagaactaga atcctcgctg
     3661 gaacagcttt tgccccgagg cggtgtcgac aagattcccg aaggcaaccc gtatcgagga
     3721 ctgctgcctt tcgaggcgga acatcgctcg ctgtttttcg gacgacgaag tgaaatcggc
     3781 acgctggtgg aacggctccg caccgatgct tgcgtactgg ttgccgccga gtcaggcgtg
     3841 ggcaaatcgt cgctgtgtcg cgctggtgtc ctgccattgg ttgcggaagg agcactggcc
     3901 ggaggacggg cctggcgcgt cgtgtcgatg gtgcccggac ggcatccgct gcgcgcgctg
     3961 gcacaagggc tttcgtccgt ctgtcagcag agcgacgacg attttgtgcg cgacatcagc
     4021 gccgagcccg agcggctggg gcggatcttg caccagcggc tgggcaacga ccgcggtctg
     4081 ctgctgtttg tcgatcaact agaagagctg tcgacgatag ccgatccacg cgaagcccag
     4141 atcgtcggcg aagcgctggg cagtctcttg gcccggatca catcggtgcg gctgctcatc
     4201 accgtgcgca gcgattacct ggggcgggtc gcgagtctac ccagcattgg cgaggccgtg
     4261 acccgttcgc tgtacatcct gcgcccgctc ggtcccgatc gaattcgcga agccgtcgtc
     4321 ggaccagccc atgtcaaagg tgtgctgttt gaaagcacgg atctgatcag cggactggtc
     4381 gattcaactg catccaccga cggtgcactc cctcttctcc agtttacgct ggcagaactt
     4441 tgggaagccc gcgaaggcaa ccgcatcacc caagccgcac tcgacaacat tggcggtgtc
     4501 accggcgcgc tgtcccgtca cgccgaccat gtgattggca cgctaccgcc ggcccagcgc
     4561 caaagcgccc gccgagtcct gatgagcctg gtgacgctcg aaggcacgcg cgcccgtcgc
     4621 actgaagaag agcttatcaa ttccgactcc aacgcccgct cggcgcttga agcgctggtt
     4681 cgcggtcgac ttctcgtcgc acgcgacacc gccgatggca ccgcttacga agtcgcgcac
     4741 gaagcgctta tcaaaggttg gattacgctc agtcgctggc tcgaagagtt tgccgaaagc
     4801 cgtgcggtca aacagcgact cgaaaccgct gcgacagagt ggcgccgcat gtcgcgatcc
     4861 cccgatgcgc tgtggtccgc cgcccagctc gctgagcttg cgctggtcga atcggcagac
     4921 atcgcagcca aagaagccga gtttgtcgcg gcatcgtggc gcaacgtccg caagcgaacc
     4981 cttctgcgca accttctcat cggatcgctg ccacttttgg cggtactcag ctactccgcg
     5041 ttcgaataca ccgcacaccg caacctctcg atccgcattg atggttatct gcgcgacggc
     5101 aagtccgatc ttgacgcagc gcgtcgtcgc aaccgcgaag tcgatgagct gcgccgggcg
     5161 gcattttccg cgttcgattc gatgcagaac aacgacggcg aagagatgtg gaaccgtgcc
     5221 ctcgcgctca gccttgatgc cgaacgagct tattcccgcg ccggtcagac gtttgaagcc
     5281 gcgctcaccg ctgacagcac caacatccca gcgcgtgagc tgctcgcgga tgtgctctac
     5341 gaacgtgcgc tcatcgccga acgcgaccgc cgtccgcaac agctcgatga cctgcttcag
     5401 cggatggcgc tttacgatct gtctggcgcg cgcaagctca agtggaatgc gccgtcgacg
     5461 atctcggtcc gcaaaagccc cgattcagcc caggtttcca tcgctcgcta cgaacgcgat
     5521 cccaaaggtc ggcgcatcct gaccactccg cgcgacctcg gcgcagcacc gaccatttcg
     5581 acgacgatgc cgcctggttc gtatcttttg acgttgtctg ctccgggatt tgccgacgtg
     5641 cgctatccgg tctttttgac ccgcagtgaa accgtcgagc tgtctatcga cctgccggcc
     5701 caagcgcgcg ttcccgatgg ctttgtctac gtgccgcctg gccgctttct gtttggctct
     5761 ggctccgaag aaaccatgcg caagagcatg ctgaccgcgg ttcccattca tcaggtccag
     5821 accggcggtt ttctcatcgc caaacacgaa acgacgttcg ccgattggct gacattcctg
     5881 tccgcactgt cccccaccga gcgcagtcgc tatctcagca aacccacacc gagcagcttg
     5941 cctggcgccg tcggtctgac ccaacttccc ggtggtgagt gggagctgac gatccaaccg
     6001 accagcaaaa agtacatcct gcgtgaaaac caaaagctgg tgtacgacac gcgaaaaatt
     6061 caccgagagc acgactggca caagcttccc gtgagcggca tttccttcca agacgccaaa
     6121 gactacgcgg cctggctcgc caagagcggc aagctgcccg gcgcgcgcct ttgttctgaa
     6181 ctggaatggg aacgcgcagc caagggcgct gacgaccgcg agtggccgca cggtgacgac
     6241 atcttggcag gcgacgccaa cttcgacgcg acctataact tcgatgcggt gtcgatgggc
     6301 cccgatgaag tcggcagcta cccgctgtcg aagagtccgt tcggtatcaa cgacatggcc
     6361 ggaaacgcct ttgaatggac gatttcctcc ctggccaaag atgaagcttc ggtacgaagc
     6421 agcagctact tctttgccgc gatcgttcag cgcagcacca accgcaacac actggatcca
     6481 tcgatgcgca gcccaggcgt ttcgatgcgg ctgtgtgcca ccctttcgct cgccaagcca
     6541 gagcccgccc ggaactgacc accccttgcg gattggggtc ttcaaggtgg aaacgacaga
     6601 ccccaggtcc agcgcgaact ggcgattgac ttctttgcct gcgcgacgga aaccagccgg
     6661 gccacacaga caaacgcaaa aagctgggct acgctgattg tgagcacgcc ccttgcagtg
     6721 ctcaccatgg caagaggtat tcatcatgcg gctccagcgc gtagtttcat ttcttggctc
     6781 gggccttcta tttttggcaa gcggctgcgg acacgaagat ggcccagcgc tcaccctcga
     6841 tctaaatcgc ctcgattcga tgcagtccaa tgacgacgtc gtcaacggcc tcacattaaa
     6901 cggtctgacg ctcaatggtc tgacgctcaa cggtctcacc ctcaacggtc tcac
//
LOCUS       DAORGN010000240         6881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5144_length_6881_cov_6.041752, whole genome shotgun sequence.
ACCESSION   DAORGN010000240 DAORGN010000000
VERSION     DAORGN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_6881_cov_6.041752"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..803
                     /locus_tag="PKL17_19075"
     CDS             39..803
                     /locus_tag="PKL17_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNK46895.1"
                     /translation="MRVRFSGETNIGMKRKHNEDSFLIPDYERVAVVADGMGGHASGE
                     VASGMAVQCVGEYLQSTLEQPIVTWPFKLDTGVRHDTHRLTIAIKHANQRIYETAQRD
                     EEKHGMGTTIVAALFSDDRVIVAHVGDSRCYRLRRNQLIQLTEDHSLLNDYMKMKRLS
                     GEDGARFTQKNVIVRALGMKESVQVDTMIDQPQIDDIYLLCSDGLSGMVSDPGLAEIM
                     RSESDLDNACEKLIRAANDAGGTDNITAILCRIERL"
     gene            complement(881..2746)
                     /locus_tag="PKL17_19080"
     CDS             complement(881..2746)
                     /locus_tag="PKL17_19080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNK46896.1"
                     /translation="MSLFLGCSGPQTQVDGHTNPASPPDEVTVALVGTSDLHGYVEPR
                     SQQIKDRDGTSRNIDKGGLTLLGGYLANLRKRYPVLLLDGGDLFQGTLVSNLGEGKAV
                     IDGYNALGYSASALGNHEFDYGPDGPKSVPATPEDDPTGALKARIASAHFPFLSANVI
                     DKKTGLPVSWPNIYPSRIVHIAGIPIGLVGAVTEDTPRTTNILNLRDVQIASIIPAVR
                     AQAEQLRRSGVAAVVLTIHEGANCSDFHNAKDLRPCENEDGRVLPIARALHDVVDAVV
                     AGHSHAGMAHFVDGIPVIQAFAYGQAFARADLVFRNTKSGFVLDKPKTVVHRPKELCS
                     VQVPIEDEPLPPAPDKPNGDGAGNRGVPPQRVWHCDAKMLGGKELKPATYEGLPVVPS
                     EPVQLALAPHIAKAAEKRGTPLGVTMTTRLPRHFKAESPLGQFLADLTRGAAAFSTGQ
                     PVDLALQNGGGIRNELPAGKLTYGDLFEVLPFDNRLALVRMPGHALVELFRKNIQGSH
                     GVLVPSGGFRIESRCDGSELKVDMFGPDGKPVDSDRIYAVAVSDFLALGGDNFGTIVP
                     KLGQGSVQVFEDTMLREVIVGELRRYQGPFLSGSPQPHRLNLPMPRPVRCPPSNM"
     gene            3006..4055
                     /locus_tag="PKL17_19085"
     CDS             3006..4055
                     /locus_tag="PKL17_19085"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNK46897.1"
                     /translation="MLTAFYVALAILALGILIIVHEWGHYIVAKLCNMRVDRFAIGFG
                     PALFSKVRGETTFQVGAIPLGGYVQIAGLNPDDEAIAADDPRSYPNRPAWQRFLTIAA
                     GPIVNYVFAVIVFFFINFFAGVPGTMVQKLIPGQPAAASGLAEGDIITRINGHPVGMI
                     DEVRPRIAGSGGQPLEIQVRRAGELRTVVVQPVANPSGGYSIGIQMNVSPDRERLGVL
                     AAAKLAAIEPWRLTVAQVVALTNMITGRHKAKMDDFVSIIGITQMIAQQMGQRFVEGL
                     ELAAKISALLGFFNLLPLPALDGGRLVFLGIEVVTRRRVNHRVEQIVHMVGLVILLGL
                     MLLLVGKDILRLLKR"
     gene            4059..4817
                     /locus_tag="PKL17_19090"
     CDS             4059..4817
                     /locus_tag="PKL17_19090"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNK46898.1"
                     /translation="MAMREKPGQTMVKVRLGSTFLPGCVTVFAVFALTFVLSLLPVVG
                     DFVHSSLLLVPARAIGPQPWRLVTAPFVVLSPFSLLFLGLLLWSIGSAIEQRIGRRRF
                     VGWSAAVLLSSSLIVGLLGRLHGLANSGTAQLPVLLEGGPIFSMVLLAFAQLYGGLQV
                     QMWGVPQLTSGRTLAWFFLGIGLCANLLRGEWELLGANLLTLGVTFALLKNHGGQSLW
                     TLVRRTLRTEKPRKFQVLDGGRSGSDDNANKWLN"
     gene            4920..>6881
                     /locus_tag="PKL17_19095"
     CDS             4920..>6881
                     /locus_tag="PKL17_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46899.1"
                     /translation="MTSAATTKSSANPFAHTAVSDLDHDDLVDVGSLHPEARQLLQRA
                     LKQAGRGFGQLIALVGDPGAGKSHLLWWLKRQHRPESLVVTIPALPDLAQPFRYTLKQ
                     LLSGLCRVDKKGAKLDRPIDRLLWEVIFSQTYDLLDAARVGMYQGPTAVLKQLGPLCL
                     DGGKRRPIADFAEAAQKVWPEIEPGLRSYLLTLPTENSLDATARQVIVQFPYADRRAL
                     TTAWLAGEELSQKDREKIGAKQTINQEATARYVLCSLIRLATIRRPASFTILFDQADQ
                     VLEQLGQPGLHALAEVMGSLHGLTAATLLLLSCRPDTFGQFSEKQARPGPSQIKQSME
                     LISLSRIAPAQLREVVAARLQSVTASGAAAGLHPLQEADLSVLHEVDTPRAALMRLAA
                     LYSERLASGGAQAKPAAVAKPTPAPATTSKPAQAKIDPSAAKTTDNTRPVVISKAAET
                     AAKAAAEKSAKPSVKPTEKAAKTSEAVPKANAAVPVATKAAESQATPSKAWMAMSGEG
                     DALADAMAQAEKGEVSIDKARSTTHVDKSGPAVPKEAKLAPTAKVVIKDAAPKQVQSA
                     APVSRADSGDTVRRVPDEALIAKTRDAAAKATAPAATPVAKTQAPAKATATPAPAKTP
                     APAPAPVPAKAQAPAKAAAKPTPAAAPSAE"
BASE COUNT         1298 a         2013 c         2232 g         1338 t
ORIGIN      
        1 gctgaccaag catgtcctca tcgtttccca tgcggaatat gcgggtccgc ttctcaggcg
       61 agaccaacat cgggatgaag cgcaagcaca acgaggacag cttcctcatt cccgattatg
      121 aacgagtcgc cgtcgtcgcg gacggcatgg ggggccacgc cagcggcgag gttgcctcgg
      181 gtatggcggt gcagtgcgtc ggtgagtatc tccaatcgac gctcgagcaa ccgatcgtaa
      241 cctggccgtt caagctagac accggcgttc ggcacgatac ccatcggttg acgatcgcca
      301 tcaaacacgc caaccagcgg atctacgaaa ccgcgcaacg tgacgaagaa aagcacggca
      361 tgggtacaac catcgtcgca gctctcttta gtgacgatcg cgtgatcgtg gcgcacgtcg
      421 gtgacagtcg gtgctatcgg ctgcggcgca atcagctgat tcagctgacc gaagatcact
      481 cgctgctgaa cgactacatg aaaatgaagc ggctgtcggg tgaggacggg gcgcgtttta
      541 cgcagaagaa cgtcatcgtg cgggcgcttg gcatgaaaga gtcggtgcag gtcgatacga
      601 tgatcgatca gccgcagatc gatgacatct atctgctgtg ctccgacgga ctttccggca
      661 tggtgtccga tccggggctc gccgagatca tgcgttccga aagcgacctc gacaacgctt
      721 gcgaaaagct gatccgcgcg gcaaacgacg ctggtgggac tgacaacatc accgccatcc
      781 tctgccgaat cgaacgcctg tagcttccga cgatccactg ccggatcgcc tgcggtcgtc
      841 gtgatcggcg atgctgcggc gttcgtttcg cgtctgcggt ctacatgttc gacggcggac
      901 agcgcaccgg acgcggcatg ggcaagttga gtcgatgggg ctgcgggctg ccggaaagaa
      961 acggcccctg gtagcgccga agctcgccca cgatgacctc gcgcagcatg gtgtcttcga
     1021 agacttgcac cgaaccttgg cccagctttg gcacgatggt gccaaagttg tcgccgccga
     1081 gcgccaagaa atcgctcacc gcgacggcat agatgcgatc ggagtcgacg ggcttgccgt
     1141 cgggaccaaa catgtcgact ttgagctcgc tgccgtcaca gcgggactcg atgcggaagc
     1201 cgccgcttgg taccaggacg ccgtgactgc cttggatgtt tttgcggaac agctcgacga
     1261 gcgcgtgacc cggcatccga accagcgcca gccgattgtc aaacggcagc acttcaaaca
     1321 gatcgccgta ggttagcttg cccgccggca gttcattgcg aatgccaccg ccgttttgca
     1381 gcgccaaatc gactggctgt cccgtcgaaa aagctgccgc gccgcgggtc agatcggcca
     1441 aaaattgccc gagcggcgac tcggctttga agtgccgtgg cagccgcgtc gtcatcgtta
     1501 ccccaagcgg cgtgccgcgt ttttcggcgg ccttggcaat gtgtggtgcc agcgcgagct
     1561 gcacaggttc cgacggaacc accggcagac cttcgtaggt cgcaggcttt agctctttgc
     1621 cgccgagcat ctttgcatcg cagtgccaga cccgctgggg cggcactccg cggttgccgg
     1681 caccgtcgcc attgggcttg tccggtgctg gtggcagcgg ttcgtcttcg atcggcactt
     1741 gtaccgagca cagctctttg ggccggtgca ccaccgtctt cggtttgtcg aggacaaaac
     1801 ccgacttggt gttgcggaag accagatcgg cgcgggcaaa cgcttggcca taagcgaacg
     1861 cttggatgac gggaattcca tcgacaaagt gcgccattcc agcgtgtgag tgcccagcca
     1921 ccaccgcatc gacgacatcg tgcagcgcgc gggcaatcgg cagcacccga ccatcttcgt
     1981 tttcacacgg gcgcaggtct ttggcgttgt ggaagtcgct acagtttgcg ccttcgtgaa
     2041 tcgtgagcac gacggcagcg acgccacttc gccgcagctg ttcggcttgg gcacggactg
     2101 ccgggatgat gctcgcgatc tgcacatcgc gcaggttcaa gatgttggtt gtacgcggcg
     2161 tgtcttcggt gacagcgccg acaaggccga tcggaatgcc ggcaatgtgg acaatccgtg
     2221 acggataaat gttgggccac gagacaggca gtccggtttt tttgtcgatc acgttggccg
     2281 acaaaaacgg aaagtgcgcc gacgcaattc gtgctttgag cgcgccggtc ggatcgtctt
     2341 cgggcgtggc tggcaccgac ttgggaccat cgggaccgta gtcaaattca tggttgccga
     2401 gcgcggacgc gctataaccc agggcgttgt agccatcgat gacggctttg ccttcgccta
     2461 ggtttgaaac cagcgtgcct tggaacaggt cgccaccatc gagcagcagc actggatagc
     2521 gcttgcgcaa gttggccaaa tagccgccga gcagcgtcag cccgcctttg tcgatgtttc
     2581 ggcttgtgcc gtcgcggtct ttgatttgct gggatcgcgg ctcgacgtag ccatgcagat
     2641 cgcttgtgcc aaccagcgca acggtcactt catcgggcgg cgaagccgga tttgtgtgcc
     2701 catcgacctg tgtttgcggt ccgctacaac ccaaaaaaag cgacaacgcc agtgctttcg
     2761 cgactttgcg cgggccgact gattgtttgg tctgtgcttg tgggccggat ttttgccagc
     2821 gagtgacaaa cgggaagggg gggatgcgcc gattcgtgat caccgcacag cccccatgct
     2881 gtgacgctgc gtgagcatct gcacgactcg tttttagcat cgcacgttaa gttgcgcttc
     2941 tgcaaggctt ttgccgtcct ggccctcctt ggcggcgacg agtgattcag gctaagctgt
     3001 ggcgcatgct tacagcgttt tatgtcgcac tggcgatttt ggcactcggc atcctgatta
     3061 tcgtgcacga gtggggccac tacatcgtcg ccaagctgtg caacatgcgg gtcgaccgct
     3121 ttgcaattgg ctttggtccc gcgctcttta gcaaggtccg cggcgaaacc acgtttcaag
     3181 ttggcgcgat tccgctgggt ggatacgtac agattgccgg cctgaatccc gacgacgaag
     3241 cgattgcggc tgacgatccg cgctcgtatc caaaccggcc cgcgtggcag cgctttttga
     3301 ccatcgccgc cggaccgatc gtgaactacg tgttcgcggt gatcgtgttt ttctttatca
     3361 acttctttgc cggggtgccg gggacgatgg tgcagaagct gatccccggt cagcccgccg
     3421 ccgcgtcggg acttgccgaa ggcgacatca tcacccgcat caacggccat cccgtcggaa
     3481 tgatcgacga agtgcggccc aggattgccg gatcgggtgg tcagccgctc gaaatccagg
     3541 tccgtcgcgc tggcgagctg cgcacggtgg tggtgcagcc ggtggccaat ccttccggcg
     3601 gttacagcat cggcattcag atgaacgtga gccccgatcg cgaacgactc ggggtgttgg
     3661 ccgcggccaa gctggcagcg atcgaaccgt ggcggctcac cgtcgcgcaa gtcgtggcgc
     3721 tgaccaacat gatcaccggg cggcacaaag cgaaaatgga cgactttgtc agcatcatcg
     3781 gcatcacgca gatgatcgcg cagcagatgg gccagcgctt cgtcgaaggt ctggagctgg
     3841 cagccaagat cagcgcactg ctcggctttt ttaacctgct gccgctgccg gcactcgacg
     3901 ggggacgact ggtgtttttg ggcatcgaag tggtgacacg gcgtcgggtc aaccaccgcg
     3961 tcgagcagat cgttcacatg gtcggcctgg tgattttgct cgggctgatg ctgctgctcg
     4021 tcggaaaaga cattttgcgg ctgctcaagc gctaaacgat ggccatgcgc gaaaagcccg
     4081 gacagacgat ggtcaaagtc aggctcggct cgacgttttt gccgggctgc gtgacggtgt
     4141 ttgcggtgtt tgcgctgact tttgtgctgt cgcttctgcc cgtcgtcggc gactttgtgc
     4201 acagctcgct gctgcttgtg ccagcgcggg ccattgggcc gcagccgtgg cggcttgtca
     4261 cagcgccgtt tgtggtgctg agcccgtttt cgcttttgtt tttgggcctg ctgctgtggt
     4321 cgataggcag tgcgattgag cagcggattg gccggcgtcg attcgtcggt tggagcgcgg
     4381 cggtgctgct ttcgtcatcg ctcatcgtcg ggctgcttgg gcgactgcat gggcttgcga
     4441 actcggggac cgcgcagctt ccggttctgc tcgaaggtgg gccgattttt tcgatggtgc
     4501 tgctggcgtt tgcgcagctc tatggcggcc tgcaagtgca gatgtggggc gtgccgcagc
     4561 ttacgtcggg acggacgctg gcgtggtttt tcctcgggat tggtctctgt gccaacctgc
     4621 tgcgggggga atgggagctg ctcggggcca atcttttgac gctcggcgtg acgtttgcgc
     4681 tgttgaaaaa ccacggtgga cagtcgctgt ggacgcttgt gcgacgcacg ctgcggaccg
     4741 aaaaaccgcg caagtttcag gtgctcgacg gcgggcgcag cggcagtgat gacaacgcga
     4801 acaagtggct gaactagccg gccaaggcaa atccgcagaa gtttgggcgt ttttggccaa
     4861 atgagcggcc ccgccgtcgt gatatgatga ttcgacggta aaaaggctcg tcatcgctca
     4921 tgacctctgc cgctacaact aagtcgtctg ccaatccttt tgcgcacact gcggtcagcg
     4981 accttgatca cgacgatctg gtcgatgttg gttcgctgca tcccgaagcg cggcaacttt
     5041 tgcagcgcgc cctgaagcaa gctggtcgtg gctttggtca gctgattgct ctcgtcggtg
     5101 atcccggtgc gggcaagagc catcttttgt ggtggcttaa gcggcagcac cgacccgagt
     5161 cgctggtggt cacgattccg gcgctgcccg atctggcgca gccgttccgc tacacgctca
     5221 agcagctttt gtcgggcctg tgtcgcgtcg acaaaaaagg cgccaagctg gacaggccga
     5281 tcgatcggct gctgtgggaa gtgatttttt cgcagactta cgatctgctc gacgcagcgc
     5341 gggtcggaat gtatcagggg ccgacggcag tgctcaagca gcttgggccg ctgtgtctcg
     5401 acggtggcaa gcgaaggccg atcgctgatt ttgccgaagc ggcgcagaaa gtgtggccag
     5461 agatcgagcc gggcctgcga tcgtatttgc tgacgctgcc gacggaaaat tcgctggatg
     5521 caacggcgcg gcaggtgatt gtgcagtttc cgtatgcgga tcggcgggcg ctgacgacgg
     5581 cgtggctggc cggcgaggaa ctgtcgcaaa aagatcgcga aaagatcggg gcgaaacaga
     5641 cgatcaatca ggaagcgacg gcgcggtatg tgctctgctc gctgattcga ctggcgacga
     5701 ttcgtcgtcc ggcaagcttt acgatcctgt tcgatcaagc cgatcaagtg ctggaacagc
     5761 tcgggcagcc tggtctacac gcgctcgcgg aagtgatggg atcgctgcat gggctgacgg
     5821 cggcgacgct gctgctgttg tcgtgtcggc ctgacacctt tggccagttt tccgaaaaac
     5881 aggcgcggcc cggtccaagt cagataaagc agagcatgga gctgatctcg ctttcgcgga
     5941 ttgcgccagc gcagctgcga gaggtggtgg cggctcgtct gcaatcggtg acggcaagtg
     6001 gcgcggcggc gggactgcat cctttgcaag aagccgatct gtcggtgctg catgaagtcg
     6061 acacgccacg ggcggcgctt atgcggctgg cggcgctcta cagtgagcga ctggcgagcg
     6121 gcggggccca agcgaaaccg gcggcagtgg ccaagccaac cccagcgccc gcgacgacga
     6181 gcaagccggc ccaagccaaa atagatccga gcgcggccaa gaccaccgac aacacccgtc
     6241 cggtggtgat cagcaaagcg gcagagactg cagccaaagc ggcggcggaa aagtcggcca
     6301 agccgagcgt gaaaccgacg gaaaaagccg ccaagacaag cgaagctgta cccaaagcga
     6361 acgccgcagt acccgtggcg accaaagcgg cagaaagcca agcaacgccg tcgaaagcct
     6421 ggatggcgat gagcggggag ggcgatgcgc tcgccgacgc gatggcccag gccgaaaagg
     6481 gtgaagtgtc gatcgacaaa gcgcgctcga ccacccacgt cgacaagtcg ggtccggcgg
     6541 tgcccaaaga ggcgaagctg gccccgacgg caaaggtggt gataaaggac gctgcgccca
     6601 aacaagtgca gagcgccgcg ccggtcagtc gggctgattc tggcgatacc gttcgtcgcg
     6661 ttcccgacga agcactgatt gccaagacgc gcgatgcggc ggccaaggcg accgcgcctg
     6721 ctgcgacgcc agtggccaaa acccaagccc cggccaaagc gacggcgact ccggccccag
     6781 caaagacccc agccccggcc ccggccccgg tcccagcaaa agcccaagcc ccggcgaaag
     6841 cggctgcgaa gccgacgccc gctgcggccc cgagtgcgga a
//
LOCUS       DAORGN010000241         6866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5162_length_6866_cov_5.749082, whole genome shotgun sequence.
ACCESSION   DAORGN010000241 DAORGN010000000
VERSION     DAORGN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5162_length_6866_cov_5.749082"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKL17_19100"
     CDS             complement(<1..740)
                     /locus_tag="PKL17_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNK46900.1"
                     /translation="MTSCAAPGRRIVRSGLIQCSNPLNDETRPVAEIQEAMFQKHLPL
                     IEQAGQRGVQILCLQEIFNGPYFCPSQDRRWYDAAEPVPGPTVERLAPIAKKYGMAMV
                     IPVYERAIAGVYYNTAAVLDHDGTYLGKYRKIHIPHTSGFWEKFFFKPGNLGLPVFKL
                     SSGITIGVYICYDRHFPEGARILGLHGAEIVFNPSATVAGLSQYLWKLEQPAHAVANG
                     YYMGCINRVGTESPWNIGKFYGTSYFVDP"
     gene            complement(844..2967)
                     /locus_tag="PKL17_19105"
     CDS             complement(844..2967)
                     /locus_tag="PKL17_19105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK46901.1"
                     /translation="MSKKSIPSDDLSFEPDELAEVGAVLAPASQANEREEPASSKTPP
                     QAMGSFDDQVDAAFSEEETAEKQPSIAEDVDAQSHLGDYGDAAATLESIPPVSAPVVT
                     VQPSRRSTEGMVKSSAGEIEDATPPALHAESASLPSVIFDESPFGEEPKATTDDGQSI
                     TPRVAAIAAAMNGRGQPISDAVLSSSPPPSANALLVSVQNESGGLSTAGAPGRSSLSP
                     AAVVPGPADPLDDHEDPFGEAVSPSGATPVMHLDDAEFHLVEDHDDPHNLASGRTPVA
                     TSEPAVAGDPITSAPPVDGNPSTWGAASAATPPAPSQTAAVDGSWASPDSGPVTTVSV
                     DEIDTAFGEAAAAASAAEKTPDPTPPAAEPAVALPSRDSGPRRPSMGSTPSAPPPPPP
                     VANRPPPPPPPAATRPAAPPPPPAAAPKPVAVAPAAAKPLPAPVPASLPNFGEDGKLK
                     KKKRSKQWFEEIFDEDYLHTLPFLTSQQTEREVDYLLSALEMPAGSRLLDIGCGYGRH
                     AMELAARGYRTVGLDLSLPLLIRATDAARRVGVNVDFVHGDMRDMTFDSEFDGAYCFF
                     STYGYFDDETNRRVAAGICRSLRPGGRLVLELLNRDCLIGDLPTRVWWQGHGCVVLEE
                     VDFNYFTSRLEVQRQIILEDGRQLVQDISIRLYSLHEIGKILHHAGFRVLEVSGSFEL
                     RNRFYGTESRQLLIVAEKRPPDATA"
     gene            complement(2964..3773)
                     /gene="asd"
                     /locus_tag="PKL17_19110"
     CDS             complement(2964..3773)
                     /gene="asd"
                     /locus_tag="PKL17_19110"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNK46902.1"
                     /translation="MGWASRLNLPRPLRDPLYRSFSLAYGIDLGELDRPLGEFDRFDD
                     FFCRPLPDGARSISAREHALITPCDGVLSAYGSSHDGLCIHAKGQEYTVAGLLNDEVM
                     AQRFTGGMFATIYLAPHNYHRVHSPLDGDVIGYRHVPGARYPVNALSVRTVPRLFQRN
                     ERLVTYLRTDYGEVALVMVAATGVGNITVSYEPALATPAGGPDRGACIYEHGQEKPIS
                     KGAELGMFHLGSTVILLFEQGRASIDVPAGTVVRLGQPIGQLTLNKQGQTS"
     gene            4093..6117
                     /gene="ftsH"
                     /locus_tag="PKL17_19115"
     CDS             4093..6117
                     /gene="ftsH"
                     /locus_tag="PKL17_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNK46903.1"
                     /translation="MDRPFSPLAAQLVRSVFRQTQSADHSISDDQNHLLWAKSSSPRP
                     TDGRSDKTPIGKPSRSGLGGPDGNGSSPKLWLFFLLLMMLVGLPRLIGLSEKRVSFDQ
                     FAQLVEQGKLRRVSFQVDTIVGEGAEGQEGRGPQYRTGRLETAERDLVVKLNEKKIPY
                     DAVSTPSLLVTIGSWVLPMVMLFFFANWLMRRGGGIGGLSNSVFNFGKNKAQEWGESG
                     TGVTFSDVAGQREAKAELVEIIDFLTSPERYTRLGGHIPRGVILVGPPGTGKTLLARA
                     VAGEAKVPFFSISGSEFVEMFVGVGAARVRDLFAQAKEKAPSIIFIDELDAIGRARGV
                     AGPVVTHEEREQTLDQLLTEMDGFDSSTSVIIMAATNRPEILDPALLRAGRFDRRVVV
                     DRPTLEDRVEILEVHARKVVIDKSVKLEKVAAMTAGLVGADLANLVNEAALLAARRKA
                     EAVADIDFQEALERVVAGLERKSRRLSPKDKQVVAYHECGHALVATKLASADEVKKIS
                     IVPRGVGALGYTMQQPSEKEGDRYLLTRGELLDRIAVLLGGRAAEEELLSDISTGAQD
                     DLQRATQLVRRMCSEFGMSQALGLQAFDSGRGQFLDVSGYANREVSDETARAVDQEVR
                     SVLDAQYDRVRQLVRTHRASVEQAVKELQEHEVLDGDRFREIVLGDVRGS"
     gene            6117..>6866
                     /locus_tag="PKL17_19120"
     CDS             6117..>6866
                     /locus_tag="PKL17_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNK46904.1"
                     /translation="MSECRTLIVDDEETYARALQRLLGRRGIEADVATTAAEGLHRAR
                     RHPYHLVVLDYMLPDGTGLDLIPPLRQLRPTPAVLMMTAYGTIDNAVEAMRRGATDYV
                     PKSTALADVVERVVEAERVARTALKATPAPSATSHSGLAQEFLGESQSIREVRTRIQE
                     VAQSPDTTVLLCGESGTGKGVGARAIHALSARSTEPFVAIDCVALPAPLAESELFGHE
                     KGAFTGAERLKPGRLEVAGRGTVLLDEIGDME"
BASE COUNT         1338 a         2025 c         2216 g         1287 t
ORIGIN      
        1 ggatcgacga aatagcttgt gccgtaaaac ttgccgatgt tccacgggct ctcggtgcca
       61 acgcggttga tgcagcccat gtagtaaccg ttggcgacgg cgtgggcggg ctgctctagc
      121 ttccacaggt actgggacag acccgcgacg gtggcagacg ggttaaagac gatctcagcg
      181 ccgtgcaggc ccaggatgcg cgcgccttcg gggaagtggc ggtcgtagca gatgtagacg
      241 ccaatggtga tgcccgagct gagcttgaag acgggaagtc cgaggttgcc gggcttgaag
      301 aagaactttt cccaaaaacc cgacgtgtgc gggatgtgaa tcttgcggta tttgccaagg
      361 taggtgccgt cgtggtcgag cactgccgca gtgttgtagt agacaccggc aatcgcccgt
      421 tcgtagacgg ggatgaccat ggccatgccg tactttttgg cgatgggcgc gagcctttcg
      481 acggtggggc cagggacggg ctcggcggca tcgtaccagc gacggtcttg cgacgggcag
      541 aagtacggac cgttgaatat ctcctgcaga cagagaatct ggacgccacg ctgcccggct
      601 tgttcgatga gcggcaggtg tttttggaac atcgcctctt ggatttcggc gacgggacgg
      661 gtttcgtcat tcagcgggtt tgagcactgg atcaggccgg atcgcacaat gcgacggcca
      721 ggcgcggcac agctcgtcat ggtttcacct cttttgcggc gataccacag acggcataag
      781 caggtaaagc ggtgcgcgcg gacctgggaa tcggcccggc gacttccggt cagggagcga
      841 aagttaggcg gtggcgtcgg gtggccgctt ttcagcgacg atcagaagct ggcgcgattc
      901 ggtgccgtag aagcggttgc gaagctcgaa gctgccggac acctccagga cgcggaagcc
      961 ggcgtgatgc agaattttgc cgatttcgtg caggctgtag agccgaatcg agatgtcttg
     1021 gacaagctgc cgaccgtctt cgaggatgat ctggcgctgc acttcgagcc gcgacgtaaa
     1081 gtagttgaag tcgacttctt cgaggacaac gcagccatgg ccctgccacc aaacgcgggt
     1141 cggcagatcg ccaatcaagc agtcgcggtt tagaagctct aggaccaagc gtccaccggg
     1201 ccgcagcgaa cggcaaatcc cggcagcgac gcggcggttc gtctcgtcgt cgaagtagcc
     1261 gtaggtcgaa aagaagcagt aggcgccgtc gaactcgctg tcgaacgtca tgtcgcgcat
     1321 gtcaccgtgc acgaaatcga cgttcacgcc gacgcgacga gccgcatcgg tggcacgaat
     1381 aagcagtggc agcgacagat ccaacccgac ggtgcgatag ccacgcgccg ccagttccat
     1441 cgcgtgacga ccatagccac agccgatgtc gagcagccgt gatccggcgg gcatttccaa
     1501 ggcactgagc aggtaatcga cttcgcgctc ggtctgctgc gaggtcaaaa atggcagcgt
     1561 gtgaaggtaa tcttcgtcga agatttcctc gaaccactgc ttgctgcgct tctttttctt
     1621 cagcttgccg tcttcgccaa agttgggcag cgaagcggga accggggcgg gcagcggctt
     1681 ggcagcagcc ggggccacag cgacaggttt gggtgcagca gccggtggtg gcggcggtgc
     1741 agccggtcgg gtcgcagccg gcggtggcgg tggtggcgga cgattcgcaa ccggcggtgg
     1801 tggtggcggc gccgacgggg tcgaacccat gctcggacgt cgcggccccg agtcgcgcga
     1861 tggcagtgcg acagccggtt cagcggcggg tggcgtcgga tctggcgttt tttcagcggc
     1921 agatgcagcg gcagcggctt cgccaaacgc agtatcaatt tcgtcgacgc tgactgtggt
     1981 cactggcccg ctatcggggc ttgcccacga tccatcgacg gcagcggtct gactcggcgc
     2041 tggtggcgtt gcagccgacg cggcccccca ggtgctcggg tttccatcga cgggcggagc
     2101 gctggtgatc ggatcgccag cgacagccgg ttcactggtc gcaaccggcg tgcgtcccga
     2161 tgccaggttg tgcggatcat cgtgatcttc gacgagatga aactccgcat catcgagatg
     2221 catcaccgga gtcgcgcccg acggtgacac cgcttcgcca aacggatctt cgtgatcgtc
     2281 aagcgggtcg gcaggtcccg gcacaacagc cgctggtgac aaagacgatc ggcccggagc
     2341 ccccgccgtc gacagacctc ccgattcgtt ttgcaccgaa accaacagtg cattggcgct
     2401 cggaggaggc gacgaactaa gcaccgcgtc gcttatcggc tgaccgcggc cattcatcgc
     2461 cgctgcaatc gccgcaactc gcggtgtgat cgactgtcca tcgtcggtag tggctttcgg
     2521 ttcttcacca aatggcgatt catcgaaaat cactgacgga agcgatgcac tctcggcgtg
     2581 gagtgccggc ggcgtcgcgt cttcgatctc tccggcggat gacttgacca tcccttcggt
     2641 tgatcgtctc gacggctgga ccgtgacgac aggagcactc accggcggaa tcgactcgag
     2701 cgtagccgct gcgtcgccat aatcccccag atggctctgc gcatcgacgt cttcggcgat
     2761 cgagggctgt ttttcagccg tctcttcttc cgaaaacgca gcatccactt gatcatcgaa
     2821 cgaacccatc gcctgcggcg gcgtcttgct gctcgcaggt tcctcgcgct cgttcgcttg
     2881 actcgctgga gccaggaccg cgccgacctc ggccagctcg tccggctcaa acgacaaatc
     2941 gtcgctggga atgctctttt tgctcaagat gtctgaccct gtttgttgag cgttagctgc
     3001 ccaatcggtt ggcccagacg gaccacggtg ccagcgggga catcaatcga tgccctccct
     3061 tgctcaaaaa gcaggatgac tgtcgagccc aggtgaaaca tgccaagctc cgcccccttg
     3121 ctgattggct tttcctggcc gtgttcgtag atacacgccc cccgatctgg cccgcctgct
     3181 ggcgtagcca gggcaggctc atagcttacc gtgatattgc ccacgccggt agctgcgacc
     3241 atcaccagcg cgacctcgcc gtaatcagtc cgcaggtagg tcaccaggcg ctcattgcgc
     3301 tggaacagcc gtggcaccgt tcgcaccgac agcgcattga ccggatagcg cgcgccggga
     3361 acgtggcgat agccaatgac atcgccatct agcgggctgt ggacgcggtg gtagttgtgc
     3421 ggcgccaagt aaatcgtcgc gaacatgcca ccggtgaagc gctgcgccat cacctcgtcg
     3481 ttgagcagtc cagcaacggt gtactcttgc cctttggcgt gaatgcacag cccgtcatgc
     3541 gaactgccat acgcgctgag caccccatcg caaggggtga tgagcgcatg ctcgcgcgcc
     3601 gaaatcgagc gcgctccgtc gggaagtggc cgacagaaaa aatcgtcgaa gcggtcaaac
     3661 tcgcccagcg ggcgatccag ctcgcccaga tcgatcccgt atgcgagaga aaagctccga
     3721 tagagcggat cgcgaagtgg gcgcggaaga ttgagccgag aggcccagcc catgccgcgg
     3781 ctccacgcct gcttcggtag ccattgaagg agttcagatg cgatacggtc tgcgacgggg
     3841 agtttagcgg acataccagc actactatgc ggactttgcg ggggtttttc aattgatccc
     3901 ttttggcatt gcgcgcaaag caagcagcaa gtggggcggg ttgtagggaa tgctcccgca
     3961 ggcaccgcag gtgggggtag cgagtgcttc tgaacgcgag tagagtctgg gcgagacacc
     4021 ggcgctcggg cgttccgagg acggtgcagc ttcggctcct cttcgagagg ctcgctcgcc
     4081 ttggaattga ccatggatcg cccattttcc ccactcgcgg cgcagcttgt gcgcagtgta
     4141 tttcggcaaa cgcagtcggc tgatcacagc atcagcgatg accagaacca cctgttgtgg
     4201 gccaaatcgt cgtcgccgcg gccaaccgat ggtcgttccg acaagacacc gatcggcaag
     4261 ccaagccgca gtggtctggg tggccccgat ggcaatggct cgtcaccgaa gctgtggctg
     4321 tttttcctgc tgctgatgat gctcgtcggg ctgccacggc ttatcggtct gtcggaaaag
     4381 cgcgtgtcgt tcgaccagtt cgcccagctt gtggaacaag gcaagctgcg ccgggtcagc
     4441 tttcaggtcg acaccatcgt cggtgaaggc gccgaaggac aagaaggccg cggtccgcag
     4501 tatcggaccg gacggctcga aaccgccgag cgcgatctcg tcgtcaagct gaacgaaaag
     4561 aagattcctt acgatgccgt gtcgacgccg tcgctgctgg tgacgatcgg atcgtgggtg
     4621 ttgccgatgg tgatgctgtt tttcttcgcc aactggctga tgcgacgcgg cggcggaatt
     4681 ggcggcctgt ccaattcggt ttttaacttc ggcaaaaaca aagcccaaga gtggggcgaa
     4741 tcgggcaccg gcgtcacctt ttccgacgtt gccgggcagc gcgaagccaa agccgaactg
     4801 gtcgagatca tcgacttttt gacgtcaccg gaacgctaca cgcggcttgg tggacacatt
     4861 ccacgcggcg tcatccttgt cgggccgccg gggacgggca agacgctatt ggctcgcgcc
     4921 gtcgccgggg aagccaaagt gccgtttttt tcgatctcgg gatcggaatt tgtcgaaatg
     4981 ttcgtcggtg tgggtgcggc gcgggtgcgt gacttgtttg cccaggccaa ggaaaaagcc
     5041 ccgagcatca tctttatcga cgaactcgat gcgatcggac gggcgcgcgg cgtcgctggc
     5101 ccggtggtca cacacgaaga acgcgaacag acgctcgatc agcttttgac cgaaatggac
     5161 ggctttgact cgagcacgag cgtcatcatc atggcggcga cgaaccggcc cgaaatcctc
     5221 gaccctgcgc tgctgcgtgc cggacgcttt gatcggcgag tcgtcgtcga tcgccccacg
     5281 ctcgaagacc gcgtcgaaat tttggaagtc catgcccgca aagtggtcat cgacaagagc
     5341 gtcaagctcg aaaaagtcgc tgcgatgacc gcggggctcg tcggtgcgga cttggcgaac
     5401 ctggtcaatg aagcagcact tttggccgcc aggcgaaaag ccgaagccgt cgcggacatc
     5461 gacttccaag aagcgctcga gcgggtcgtc gctggtctgg aacgcaagtc gcgtcggctg
     5521 tcgcccaaag acaagcaggt cgtcgcctat cacgagtgcg gacacgcctt ggtggcgacg
     5581 aagctggcgt ccgccgacga ggtcaaaaaa atcagcatcg ttccgcgcgg tgttggcgca
     5641 ctgggctaca cgatgcagca accgagtgaa aaagaaggcg atcgctatct tttgacgcgc
     5701 ggcgagctgc tcgatcggat tgcggttctg ctcggtggac gagcggcgga agaagagctg
     5761 ctcagcgaca tttccaccgg cgcccaagac gatttgcagc gcgccaccca gcttgtcaga
     5821 cggatgtgca gcgaatttgg catgtcgcaa gcgctcggtc ttcaggcgtt cgacagtggc
     5881 cgcggccagt ttcttgatgt ttcgggctac gcaaatcgcg aagtcagcga cgaaacagcc
     5941 cgcgccgtcg atcaggaagt caggtccgta ctcgacgccc aatacgatcg cgtccgtcag
     6001 cttgttcgta cccatcgggc cagcgtcgaa caagcagtca aagaactcca agagcacgag
     6061 gttctcgacg gagatcgttt ccgcgagatc gtcttaggcg atgtaagagg tagctgatga
     6121 gcgagtgccg caccctaatt gtcgacgatg aagagacgta tgcgcgggca ctccagcgct
     6181 tgctcggtcg ccgaggaatc gaagccgacg tagcgacgac agccgccgaa ggactgcatc
     6241 gcgcccgccg ccatccgtat cacctggtgg tgctcgacta catgctgccc gacggaactg
     6301 gactcgactt gattccgccc ctgcggcagc ttaggccgac gccggcggtg ctgatgatga
     6361 ccgcatatgg aaccatcgac aacgccgtcg aggcgatgcg acggggcgcg accgactacg
     6421 tacccaagtc gaccgcgctc gccgatgtcg tcgaaagagt cgtcgaagcc gagcgggtcg
     6481 cacgcaccgc gttaaaagcg acaccggccc catctgcgac gagccatagc ggcctggcgc
     6541 aagaattttt gggcgaatcg cagagcattc gggaagtccg cacgcgcatc caagaggtcg
     6601 cgcagtcgcc ggatacgacg gtgcttttgt gtggtgaaag tggcaccggc aaaggcgtgg
     6661 gcgcacgcgc cattcatgcg ctgtcggcgc gatcgacgga accgtttgtc gccatcgact
     6721 gcgtcgccct gcccgcaccg cttgccgaaa gtgagctgtt tggacacgaa aaaggcgcgt
     6781 tcaccggcgc ggaacggctc aagcctggtc ggctcgaagt ggcgggtcgc ggaaccgtgc
     6841 tgctcgacga gatcggcgac atggaa
//
LOCUS       DAORGN010000242         6756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5306_length_6756_cov_6.461871, whole genome shotgun sequence.
ACCESSION   DAORGN010000242 DAORGN010000000
VERSION     DAORGN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_6756_cov_6.461871"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKL17_19125"
     CDS             complement(<1..364)
                     /locus_tag="PKL17_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46905.1"
                     /translation="MSRRAFDPLGFSASVFALASLLSCNPAPATSDDGPADLSTIPPT
                     SPPDFASVAGDSVPAGAVAFFQRKDCPQGWEKLGAASGRTLVPTVGADPVDVSVGTPL
                     QDSEERSHSHAISGTINLS"
     gene            complement(373..831)
                     /locus_tag="PKL17_19130"
     CDS             complement(373..831)
                     /locus_tag="PKL17_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46906.1"
                     /translation="MHFGKIHSQTWKWLAVPVLAAGLAVGIRTASGDDPTGGLDDAPR
                     GMLSFVAPDVTACPQGWRAAQETSGRLVVGVTDSDAVGKLIGTPLLTQEDRTHSHAFS
                     VTADLPYKSISAANGGNNQGAGAGKYPDDGQTELAPTGLPFVQLLGCVKQ"
     gene            complement(905..1810)
                     /locus_tag="PKL17_19135"
     CDS             complement(905..1810)
                     /locus_tag="PKL17_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46907.1"
                     /translation="MRFLTARVELSPLALAFTALVTMTGCPKPSAIKVPPPPAGDLLR
                     FVAKPSDKLTGKVSVRFEDGDLGVKKPAVYAFLFKEEHVAQTVDASGNMNFSASFFDV
                     EPVGDSAKEKKAGEPLARALQSVKIAYTMSSRGDVTNFDVKIPDDIKDTKEGKQMERQ
                     LGYVRLIAQWVYGADRGPQFDPGHIESGKTWPIRASIPTPTGGSKNWDMTCAYTKKEQ
                     GVAYINMEGKVSGENQGTKLSGEVKGELRLRIGEGRLTYHDIDTTSTFRAGDATSGGH
                     QVHIHVTWEAEQAAVPTGDGTAVMP"
     gene            1910..2473
                     /locus_tag="PKL17_19140"
     CDS             1910..2473
                     /locus_tag="PKL17_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46908.1"
                     /translation="MWIPLAHLFDRLRQPKTRAFEVRRLREKLRDEDMAQAGPDERAC
                     AAELLRLRKLLKAAIGEVQSCRTCAIGHPPPHGHFAGGHCCGLQTEDAFNDDEIAALR
                     LSGSLPRDLRLPRGDHAGCAFRGPTGCSLRVENQPNLCVRYLCPDLFREVYGRGDGAK
                     IEALCDEMEAVYLRFIKLRAARLAADE"
     gene            complement(2477..3166)
                     /locus_tag="PKL17_19145"
     CDS             complement(2477..3166)
                     /locus_tag="PKL17_19145"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNK46909.1"
                     /translation="MTKLGLVLWVILSCRLAYADTEGWLIVDSRLPVYSSADDRHKLS
                     LRVMTDFRVAARAHGLQQALMRGGFAWEPRPWLLLGSQTNANVQTSDGQKFIREWRQE
                     LEATVAMPMLPLLSFAHRQRLELRITDGNVSPRHRILLRLNLTLRSQVQPYVWDEVMV
                     TPLPYWLNQNRFSVGVAWRPRKNFLLELGYLWRLRNVGAAGLVSDHAPRLAITFIPSS
                     VEELGPYITSE"
     gene            3211..4428
                     /gene="hemW"
                     /locus_tag="PKL17_19150"
     CDS             3211..4428
                     /gene="hemW"
                     /locus_tag="PKL17_19150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNK46910.1"
                     /translation="MNARTDHPGFGVYVHFPYCRQRCAYCDFAVAVRPDIPHQEYLDA
                     LIAELSAKAPLFAGRRLRSVYFGGGTPSLWRPDCLSIALREVLSRFDLETESELEVTL
                     ECDPGDVSLAQLSAWRHAGVCRLSIGAQTFSDAQLKTLRRQHASHQIGELVTLARRAG
                     FANLSIDLLIGMSGQTESQQIEDLQKLLILNPEHVSLYQLTVEPHTFLAVQIRRGNAQ
                     PPSPDQQADSYQRAIETLSRAGFRQYEISNFARALPSDPDYRSAHNRLYWTHGEYLGI
                     GVSAHSFRQLPDGRGERFASIRGPSSYLRTWQNPWPGEFRLSAENPSLSLYELREPEA
                     LWREALWLRLRELDGVTWSDFVALHKVDPRRVCQSIIADLVARDLVLADENSLRLSAR
                     GVLFADDVGAAFL"
     gene            complement(4432..>6756)
                     /locus_tag="PKL17_19155"
     CDS             complement(4432..>6756)
                     /locus_tag="PKL17_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK46911.1"
                     /translation="QAQDGRLRPVVMDFGIAREGKESSGLTEAGMVMGTPNYMAPEQA
                     AGLVEQIDRRSDVYSLGATLYELLTGRPPIVGETVVDVLFKVLQEDPVPPRQLQRSVP
                     VDLETIALKCLAKEQSLRYDSAKALAEDLQRYVNGEPIVARKASLAYRLGKRIRKQKS
                     LFALSLLSLFFISALVTDRLRVHFQHARQAKIVKAQIERARVLGQDAKETEWFMRAVY
                     ELPMHDVTREQEIMRSRMRQLEEQIDSLGREGGQLAHYALGRGYMALQSSDLALHHLQ
                     KAMELGKATPELHYALGRLMGERFLETLRGERRRGDVEWLRARKRKLEQEFLQPAMEH
                     LAQSRGVKLESAELVDGLLALYQARYEKANAHADAALASAPWLYEAYKLKGDVWQARA
                     LAQYIGGATEKAAADLVLAIAEYDQAAERGRSDISVLEARAEAWGHLLQQLYETHRPF
                     DDMIPKAQSACQQAITVAPKNDVGYRELAGIHLLVAQSLFDNGKDPRPHVKELTDVAK
                     AGLRNGRGDPGLENAMGDGLLLVLFYEDGRGLPLSVDVNEAIDHQRRAIALNPSYLWA
                     YNGMAGLYLFRSERAQKQNTDPREDLQTAIRYSKEAVRLSPNYGTAYSNLTYFYMRLA
                     SYQLEHKQGIDDAIAAGIAYGQECQRRKPKLSDCAANLALLHLTRARSLSTQPQMDAE
                     FLAAVKETLAQLDSAEQKGDSSLELHQSRARVQLLYAQHQIHHSQSADTACSRAKKSL
                     DACFSLVADDAQCKIIERELGILCGKQNLGKTKP"
BASE COUNT         1266 a         2017 c         1987 g         1486 t
ORIGIN      
        1 tcgacaggtt gatggtgccg gaaatcgcat ggctgtggct gcgttcttcg ctgtcttgca
       61 gtggtgtccc aacgctgaca tcgactggat cagccccgac ggtgggcacc agggtgcgac
      121 ccgatgcggc gccgagcttt tcccagcctt gcgggcagtc tttgcgctga aaaaatgcga
      181 cggccccggc gggcaccgaa tcgcctgcga ccgacgcaaa gtccggtggc gacgtcggtg
      241 ggatcgtcga caaatctgca ggtccgtcgt cgctcgtcgc gggcgcggga ttgcaggaca
      301 aaagactggc cagcgcgaag acggatgccg aaaacccaag tggatcgaag gcgcgacggc
      361 tcatgttgga cctcactgct tcacacagcc gaggagctgc acaaagggga gcccggtcgg
      421 cgcaagctcg gtttgaccgt catcggggta cttgccagcg ccggcgccct ggttgttgcc
      481 gccgttggcc gccgagatcg acttgtaggg cagatccgca gtcaccgaaa acgcatggct
      541 gtgggtgcgg tcttcttgtg tcaaaagcgg cgtgccaatc agcttgccca ccgcgtcgct
      601 atcggtgacg ccgaccacca gccgcccact ggtttcctgc gctgcacgcc agccttgcgg
      661 acaggcggtg acgtcggggg cgacaaatga cagcatgccg cgcggcgcgt cgtcgagacc
      721 tccggtggga tcatcgccac tggcggtgcg aattccgacg gccagacccg ccgccaagac
      781 cgggacggcc agccacttcc acgtttgtga gtgtattttc ccgaaatgca tgtccggagc
      841 ctatgccgga cgcggggatg aaggaagccg aagatggtgg tggcagcgcg aaagcgggca
      901 ggggctacgg catcacagcg gtgccatcgc cggttgggac agcggcttgc tcggcttccc
      961 aggtgacgtg gatgtggact tggtggccgc cggacgttgc gtcgcccgcg cggaacgtcg
     1021 acgtggtgtc gatgtcgtgg taggtcaggc ggccttcgcc gatgcgcagc cgcagctcgc
     1081 ctttgacttc gcccgacagc ttggtgccct ggttttcacc cgacactttg ccttccatgt
     1141 tgatgtacgc gacgccctgc tcttttttgg tgtaggcgca ggtcatgtcc cagtttttcg
     1201 atccacccgt cggtgtcgga atcgacgcgc gaatcggcca ggtcttgccc gactcgatgt
     1261 gaccgggatc aaactgcgga ccgcgatcgg ccccgtacac ccactgggca atcagacgga
     1321 cgtagccgag ctgccgttcc atctgcttgc cttctttggt gtctttgatg tcgtcgggaa
     1381 ttttgacatc gaaattggtc acatccccgc gagagctcat cgtgtaggcg attttgaccg
     1441 actgaagcgc ccgtgcgagc ggctcgccag ccttcttttc cttcgccgaa tcaccgacgg
     1501 gctcgacgtc aaaaaacgac gccgaaaagt tcatgttgcc cgatgcatcc acggtctggg
     1561 cgacgtgctc ttctttgaac agaaaggcgt agaccgcggg ctttttgaca cccaggtcgc
     1621 cgtcttcgaa gcgaacgctg actttgccag tgagcttgtc gctgggcttg gcgacaaagc
     1681 gcagcaggtc acccgccggc ggcggcggaa ccttgatggc ggacggcttc ggacagccgg
     1741 tcatcgtcac aagtgcggta aatgccaatg ccagggggga tagctcgaca cgagcagtaa
     1801 ggaaacgcat cggcttaaat gtatcggaaa taacgaattt tagtcaaggt cttcatcgag
     1861 ttgcggcgct tggcttgtct gtcgagaaac ccgtagaatt ggcccgctga tgtggatacc
     1921 gctcgctcat cttttcgacc gactgcgcca gcccaagacg cgagcgtttg aagtgcgacg
     1981 gctgcgcgaa aagctgcgcg acgaagacat ggcgcaagct gggcccgatg agcgcgcgtg
     2041 tgcggcggag ctgctgcgtc tgcgcaagct gctcaaagct gccatcggtg aggtgcagag
     2101 ctgtcggacc tgtgccattg gacatccgcc gccgcatggt cactttgccg gcggtcactg
     2161 ctgcggtctg caaaccgagg atgcatttaa cgacgacgag atcgcggcgc tgcgactttc
     2221 gggaagtttg ccgcgcgatc ttcgcttgcc gcgcggggat catgccggtt gtgcttttcg
     2281 cggaccgact ggctgttcgc tccgcgtcga aaaccagccc aacttgtgcg tgcgctatct
     2341 gtgtccggac ttgtttcggg aagtgtacgg gcgcggcgat ggggccaaaa tcgaagcgct
     2401 gtgcgatgaa atggaagcgg tctatctgcg ctttatcaag ctgcgcgcgg ctcgtcttgc
     2461 tgccgatgaa tagtcgctat tcgctggtga tgtaggggcc cagttcctcg acgctggatg
     2521 ggatgaatgt gatggcgagc cgcggtgcgt gatcgctgac caaaccggcg gcaccgacgt
     2581 tgcgcagccg ccacagatac cccaattcca gcagaaaatt tttgcgcgga cgccaggcca
     2641 caccgacgga aaatcggttc tgattcagcc aatacggcag cggcgtcacc atcacttcat
     2701 cccagacata cggctggact tgcgagcgca gcgtcagatt cagccgcagc agaatgcgat
     2761 gccgcggact gacattcccg tcggtgattc gcagttcgag gcgctgccgg tgggcaaacg
     2821 acaaaagcgg cagcatcggc atcgccaccg tcgcttccag ctcttgccgc cactcgcgga
     2881 tgaacttctg accgtcgctg gtttgcacgt tcgcgttggt ctgactgcca agcaaaagcc
     2941 acgggcgcgg ttcccaagcg aaaccgccac gcatcagcgc ctgctgaagc ccatgcgcac
     3001 gcgctgcaac gcgaaaatcg gtcatcaccc gcaggctcag cttgtgccga tcgtccgcgc
     3061 tcgaatacac cggcagccgc gaatcgacga tcagccaccc ctctgtatcg gcatatgcca
     3121 accgacagga cagaatgacc cacagaacca ggccaagctt cgtcatagaa cctgctattt
     3181 tgcgtgcact tgacaggcaa tggaaccccg atgaacgcgc ggactgacca ccccggattt
     3241 ggtgtgtatg tacactttcc gtactgccgt cagcgctgtg catactgcga tttcgccgtc
     3301 gctgtgcggc ccgacattcc ccatcaggaa tacctagatg cgctgatcgc cgagctgtcc
     3361 gccaaagccc cactgtttgc aggaaggcga ctgcgttccg tttattttgg tggtggtaca
     3421 ccgtccctgt ggcgtcccga ttgtctttcc atcgcgctgc gtgaagtgct ttcgcgcttt
     3481 gacttggaaa cggaatccga actggaagtg acgctggaat gtgatcccgg tgacgtgtcg
     3541 ctggcgcagc tttccgcgtg gcgacatgct ggggtctgtc gtctgtcgat cggcgcacag
     3601 acgttttcgg atgcacagct aaagacgctg cgtcggcaac atgcatcgca tcagatcggc
     3661 gagcttgtca ccctggcgcg ccgtgcgggc tttgcgaact tgtcgatcga tctgctgatc
     3721 ggaatgagcg gacagacgga atcacagcaa atagaggact tacaaaagct tcttattctg
     3781 aatccggaac atgtctcttt gtatcagctc accgtcgagc cgcatacctt tcttgcagtg
     3841 cagattcggc gcggaaacgc ccagccacca agtcctgatc agcaagctga ttcctatcag
     3901 cgtgccatcg agacgctgtc gcgagccggt tttcgccagt atgaaatatc caactttgca
     3961 cgcgcactgc cttctgatcc ggactaccga tcggctcaca atcggctgta ctggacgcat
     4021 ggtgaatact tgggaatcgg cgtctcggcg cattcctttc gccagcttcc ggatggccga
     4081 ggagagcgtt ttgcaagcat ccgcggaccg tcttcctatc tgcgtacttg gcagaatcct
     4141 tggccggggg aatttcgtct atcagcagaa aatccgtcgc tgagcctgta tgaactgcgc
     4201 gaacccgaag cgctgtggcg cgaagcgctg tggctccggc tgcgcgaact ggacggcgtc
     4261 acatggtcgg attttgtcgc gcttcacaag gttgatccgc gccgtgtctg tcaatcgatc
     4321 atcgcggatt tggtcgcgcg tgatttggtt ttggcagatg aaaattcgct gcgcttgtct
     4381 gcgcgtggag tgctgtttgc cgatgacgtt ggcgccgcgt ttttgtaatc gctagggctt
     4441 tgttttcccc aggttctgtt tgccacacag gatgccaagc tcgcgctcga tgatcttgca
     4501 ctgcgcatca tcagcgacga gagaaaagca agcatccagg ctctttttgg cgcggctaca
     4561 cgccgtgtcg gcgctttggg aatggtgaat ttgatgctgt gcgtaaagca attgcacccg
     4621 cgcgcggctc tgatgaagtt cgagcgagct gtcacctttt tgttccgcgc tatcgagctg
     4681 cgccagcgtt tccttcaccg cggccagaaa ttctgcgtcc atctgcggct gcgtagacaa
     4741 cgatcgggcg cgggtcagat gcaacagtgc caagtttgca gcgcagtcac ttagcttcgg
     4801 tttccgtcgc tgacattcct gtccatacgc gattcctgcc gcgatggcat cgtcgattcc
     4861 ctgcttgtgc tcaagctggt agctggccag tcgcatgtaa aaatacgtca agttcgagta
     4921 ggccgttcca tagtttgggg acagccgcac cgcttctttg ctgtagcgga tcgcggtctg
     4981 caaatcttcg cgtggatcgg tgttctgttt ctgtgcgcgc tcgctgcgaa acagatacag
     5041 tccggccatt ccgttgtagg cccacagata gctcggattc agtgcgatgg ctcgtcgctg
     5101 atgatcaatt gcttcgttca catcgacaga tagtgggagc cctcttccgt cttcgtaaaa
     5161 cagcacgagc agaagaccgt ctcccattgc attttcaagc cctggatcac cgcgaccatt
     5221 gcgcagtccg gctttggcaa cgtcggtcag ttccttgaca tggggtcgag gatcttttcc
     5281 gttatcaaac agggattgtg cgaccaaaag atgaatcccg gccagctctc ggtagccgac
     5341 atcatttttc ggagcgacgg tgatggcttg ttggcaagcc gactgggctt tcggaatcat
     5401 gtcgtcgaac ggacgatgcg tttcgtaaag ctgctggagc agatgtcccc acgcttctgc
     5461 gcgcgcttcc agaaccgaaa tgtcactgcg tcctcgctct gctgcttggt cgtattccgc
     5521 gatcgccaag accaagtccg ccgcggcttt ttccgttgcg ccgccgatgt actgcgccag
     5581 cgctcgcgct tgccagacat cccctttgag tttgtaggct tcataaagcc aaggcgcgct
     5641 tgccagcgcc gcatccgcat gcgcattggc cttttcatag cgcgcttgat aaagagccag
     5701 aagtccgtcg acaagctccg ccgactcgag cttgacgcct cgactttgcg ccaggtgttc
     5761 catcgcaggt tgcaaaaatt cctgttccag cttgcgtttt cgcgcgcgca gccactccac
     5821 atcaccgcgc cgacgttctc cgcgcagcgt ctccaaaaag cgctctccca tcagccgacc
     5881 cagcgcatag tgaagctccg gagtcgcctt tcccagctcc atcgcttttt gcaaatgatg
     5941 cagcgcaaga tcactcgatt gcagcgccat gtagcctctt cccagcgcgt agtgtgcgag
     6001 ctgtcctcct tctcgcccga ggctgtcgat ctgttcttcg agctgtcgca tgcgcgaccg
     6061 catgatctct tgttcgcgcg tcacgtcatg catcggcagt tcatagaccg cgcgcatgaa
     6121 ccactctgtt tccttggcat cttgtccaag tacgcgggcg cgttcgatct gtgctttgac
     6181 aatcttcgct tggcgcgcgt gctgaaaatg tacgcggagt ctgtctgtga caagcgccga
     6241 aatgaagaac agagacagaa gagacagggc aaacagagac ttctgcttgc ggatccgctt
     6301 tcccagtcga tacgccaggc tggccttgcg cgcgacgatg ggctcgccat taacgtatcg
     6361 ctgaaggtct tcggccagtg ctttggcgct gtcgtagcgc agactctgtt ccttggccaa
     6421 acacttcagg gcaatcgttt ccagatccac aggcacagat cgctgaagct gtcgcggtgg
     6481 caccggatct tcttgcagga ctttgaacag cacatcgacc acggtttcac ccacgatagg
     6541 tggccgtccg gtcagcagct cgtacagcgt cgcacccaga ctgtagacat cgctgcgccg
     6601 atcaatctgt tcaacaagcc cagccgcctg ctcgggagcc atgtagttgg gagttcccat
     6661 caccatgccc gcctcggtca gaccgctgct ctctttgcct tcgcgggcga tgccaaagtc
     6721 catcacaacc ggacgcagcc gcccatcctg cgcctg
//
LOCUS       DAORGN010000243         6674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5420_length_6674_cov_6.404442, whole genome shotgun sequence.
ACCESSION   DAORGN010000243 DAORGN010000000
VERSION     DAORGN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5420_length_6674_cov_6.404442"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="dnaB"
                     /locus_tag="PKL17_19160"
     CDS             <1..1074
                     /gene="dnaB"
                     /locus_tag="PKL17_19160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNK46912.1"
                     /translation="ENIAHHARIVREKATARRLILAASTIQERGYSDEIDVDGYIDQA
                     QQAVFEIANRSNRQSYEPVRRVLHTAIKALEQRFSRKQAVTGVATDYMKFDEMTAGFQ
                     PGDLVIIAARPSMGKTAFVMNCAQNAALNHGVPTLVFSLEMSKESLIERVLCSEARVE
                     SQKLRSGFLETRDWIGITKAAGRIAEAPMWIDDGGAPSLMEIRAKCRRWRSDSSIFPT
                     GLDQLGLVVIDYLQLMHGSPQGRDQSREREISEISRGLKALAKELKVPVLALSQLNRS
                     VESRADKRPMASDLRESGAIEQDADVICFIYRDEYYNKDSPDKGVAEIIIGKQRNGPT
                     GTARLAFLNQYTRFENLAEDRAD"
     gene            complement(1084..1830)
                     /locus_tag="PKL17_19165"
     CDS             complement(1084..1830)
                     /locus_tag="PKL17_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46913.1"
                     /translation="MCCFSRPVQSVSATKIFSRDAGNGKQFLVYSMTYRAGEDLAMVL
                     PLPTPPGVKEDAVQFVDLHEYEGFFRDLESGFPQPVAVSRSRSIPANSIPAPAQLAVV
                     SVGSFEASFVPQVKDFARLDPRFRMPEGIWDKLPQYKQASFAVFKLKQSATSVHPMAF
                     VFPRADAQKLFFPTVHIHDGKVHDKAGFDHSLYCQVGHEHSRAQGWIESPELASSFVN
                     VAKSKGIIDGGAHCYKRELHGNLKNQDTYL"
     gene            complement(1853..2527)
                     /locus_tag="PKL17_19170"
     CDS             complement(1853..2527)
                     /locus_tag="PKL17_19170"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK46914.1"
                     /translation="MLGKLIGIFTHALGRSCWADERIVDANLAASASGHTVEFSIYPS
                     DATIQGEIDRLREKAQAARQAELALLETQLREKQRTILQMAMPAVQLWDGILLLPLVG
                     EIDSHRASMLMDSALSAITKHAARVVIIDITGVPFVDSHVASSLIRTVQAARLLGVES
                     LLVGISAEVARSLVLLGIDLQYVQTFSTLESGLKYALAKLNYKLLRRETESQSESALP
                     KPQRRV"
     gene            complement(2512..2937)
                     /locus_tag="PKL17_19175"
     CDS             complement(2512..2937)
                     /locus_tag="PKL17_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46915.1"
                     /translation="MEKVFRATIGSVPFEADLEEGTQSFFGIPSALFWLDPSLYRMLS
                     PLVQEAGSELARLLIALESSRGTKEDYEFMVQKLGPTFSEGFLAWGKAVGVAGWGHFE
                     LPSFEPKHGTAVVRVKNAGSCARKRMRRSAGAVLFCSAS"
     gene            complement(3039..4037)
                     /locus_tag="PKL17_19180"
     CDS             complement(3039..4037)
                     /locus_tag="PKL17_19180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK46916.1"
                     /translation="MQLPRPEKPCRPPLWAKSGYLQSALGNYLPKPKLTQPWRREEIH
                     LPDGDRLVARVYAPKEGSAPPNRKTVICVFHGLGGHGDRPYMQRVVDLGTARGCEVFS
                     VDHRGCGQGRGLAKGIYHSGVASDLSAVFAAVRKKQPDAAIIGVGFSLSANALLLCLG
                     DGHGGPHHHPDAAIAVNPPINLSRCADLICAPNHRLFNLYFLRGCVRYVRQREADGLH
                     EKGRFPVHLRMSLKTFDDVFTAPLGGFKSGQDYYERSSALPHLSKITRPTVILHAEDD
                     PFIDPTDFVQAQLGPGIHLHMEKHGGHLGYICADQPYRRWLDYGMGHYLDQLLSRL"
     gene            complement(4041..4946)
                     /locus_tag="PKL17_19185"
     CDS             complement(4041..4946)
                     /locus_tag="PKL17_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNK46917.1"
                     /translation="MSFDLKSYLSARRDLVEAELAKLGKLKALVHGDTGFAPGKLAEA
                     MNYSLLAGGKRLRPILAMAGCEAMGKPPTMALHFGCAVEMIHTYSLIHDDLPAMDNDD
                     FRRGRPTNHKVYGEALAILAGDALLTDAFALAAQSPAPAAVLVEIIRELSFAAGSPGM
                     VGGQVIDVEATGKKVDLDHLKVLHAMKTGALFDVSLRGGARLGGATDEQLGHIRTYAQ
                     ALGLAFQIVDDVLDVTADLAQLGKDPGSDREAGKTTYVDLLGVEGAMAHARQVMDAGI
                     LALAPFGVHAEPLRALGNYTVERNN"
     gene            5071..6081
                     /locus_tag="PKL17_19190"
     CDS             5071..6081
                     /locus_tag="PKL17_19190"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNK46918.1"
                     /translation="MRHLELKVGTLIVVSGGLLAMFIAILGGFSFGPTSRIYVDYDFS
                     GNIHEGAPVKISGIKVGKVEKIEFFGGELDPQVNRRVQVRLTVQIEERARKAIHDDAE
                     FFVNTQGVLGEQYLEIAPGRHENTQLPPGSKVKGVDPPRSDLIIARLYEFLDAVTRLL
                     QEDKESLRDLLRSAAKVARSLDKLLGENEAEVKKLLTDLDRLTAETAELVAKLDKGVG
                     TGEELHKTLANIESISTELKTELAPLLQKTKRALDGVGDVAQLLGPEEKKKLQRSLDD
                     LLVLSGKVSQVAGDAQSLLSDVRRGKGTVGALIVDQQIYDDLKELTRDLKRNPWKFFW
                     KE"
     gene            6085..>6674
                     /locus_tag="PKL17_19195"
     CDS             6085..>6674
                     /locus_tag="PKL17_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46919.1"
                     /translation="MSLGPPFDAGNQKLHLLLGGVTVLLLSFLVAVALLTADHSVGKS
                     VTLHLLVRRSGQLYTGAEVHLAGQRIGELTAIRRYHPSEQDPAERRARGTQVELELRI
                     LRGPAGRLFHNSTFVARNPTLLSPAVIEVGPPDLGQDKGPPIADGDFLYGIDPPDLDQ
                     LLRRIYDSLTVILTEAQTLRPEWQEAAAAFVGLQRR"
BASE COUNT         1348 a         2070 c         1982 g         1274 t
ORIGIN      
        1 gaaaacatcg cgcaccacgc tcgcatcgtg cgcgaaaaag cgacagcgcg acggctgatc
       61 ttggccgctt ccacgattca ggaacgcggc tattccgatg agattgacgt cgacggctac
      121 atcgaccaag cccagcaagc ggtgttcgag attgccaatc gatcgaaccg ccaaagctac
      181 gaacccgtcc gtcgcgtcct gcacaccgcc atcaaggcct tggagcagcg cttttcgcgc
      241 aagcaagcgg tcactggcgt cgccaccgat tacatgaagt tcgacgagat gaccgccggt
      301 tttcagcccg gcgacctggt catcattgcg gcgcgtccat cgatgggaaa gaccgcgttt
      361 gtcatgaact gcgcccagaa cgccgcgctc aaccatgggg tgccgacgct ggtgttctcg
      421 ctcgaaatgt ccaaagagtc gcttatcgag cgtgtgctct gcagcgaagc gcgggtcgaa
      481 tcacagaagc tgcggtcggg atttttggaa acccgcgact ggataggaat caccaaagcg
      541 gcaggacgga ttgccgaagc gccgatgtgg attgatgacg gcggcgcgcc ctcgctgatg
      601 gaaattcgcg ccaaatgtcg tcgctggcgt tcggattcct cgatcttccc aactggactt
      661 gatcagcttg gacttgtggt gatcgactac cttcagctga tgcacggatc gccgcaggga
      721 cgcgatcaga gccgcgagcg cgagatcagc gaaatttcgc gtggtctcaa agcgctggcc
      781 aaagagctca aggtgccggt gctggcgctg tcgcagctca atcgatcggt ggaatcccgc
      841 gccgacaagc gaccgatggc gtccgatctt cgtgaatccg gcgccatcga acaggacgcc
      901 gacgtcatct gctttatcta ccgcgacgag tattacaaca aggattcgcc cgacaaaggc
      961 gtcgccgaaa tcatcatcgg caagcagcgc aacggcccca ccggaaccgc gcgcctcgca
     1021 ttcctaaacc agtacacccg cttcgaaaac ctcgccgaag atcgcgccga ctgaccgcgc
     1081 cgcttacaga tacgtgtcct ggtttttcag attgccgtgc agttcccgct tgtagcagtg
     1141 ggcaccgccg tcaatgattc ccttgctctt ggcgacgttg acaaacgagc tggcaagctc
     1201 gggcgattcg atccagcctt gcgccctgct gtgctcgtgg ccgacttggc agtagaggct
     1261 gtgatcaaag cccgccttgt cgtggacttt tccgtcgtgg atgtgcactg tcggaaaaaa
     1321 cagcttctgc gcgtcggcgc gcggaaacac aaacgccatc ggatgcacgc tggtggcgct
     1381 ttgtttcagc ttgaacacgg caaagctcgc ttgtttgtac tgcggcagct tgtcccagat
     1441 tccttctggc atgcgaaacc gcggatcgag ccgcgcgaag tctttcacct gcggcacaaa
     1501 cgacgcttca aagctgccga cgctgaccac agcaagctgc gctggcgctg ggatgctgtt
     1561 ggccggaatc gagcgcgagc gcgacaccgc aacgggctgc ggaaagcccg attccaaatc
     1621 gcggaagaag ccttcgtact cgtgcagatc gacaaactgc accgcgtctt ccttgacccc
     1681 cggtggcgtc ggcagcggca acaccatcgc caagtcttca cccgcgcgat aggtcatgct
     1741 gtagaccagg aactgttttc catttcctgc atcgcgcgaa aaaatcttgg ttgctgatac
     1801 ggactgaacc ggacggctaa agcagcacat gacacactcc gctggctgtg aatcagacgc
     1861 gacgctgcgg cttgggaagc gcggactcgg actggctttc cgtttcgcgc cgcaacagct
     1921 tgtagtttag cttggcgagc gcgtatttca ggccgctttc cagcgtcgaa aacgtctgaa
     1981 catactgcag gtcgattccc aacagcacca ggctgcgcgc aacttccgcg gaaatgccaa
     2041 ccagcagact ctcgaccccg agcagtcgtg ctgcctgcac cgtccgaatc agcgagctgg
     2101 cgacgtggct gtcgacaaac ggcacgcccg tgatgtcgat gatgaccacc cgcgccgcat
     2161 gcttggtaat cgccgacagc gcactgtcca tcagcattga agcccggtgc gaatcgattt
     2221 ctccgacgag cggcaagagc aagatgccgt cccagagctg caccgctggc atcgccattt
     2281 gcaaaatcgt ccgctgtttt tcacgaagct gggtttcaag cagcgcaagc tctgcctgcc
     2341 gggccgcctg ggctttttcg cgcagccggt cgatctctcc ctgaatcgtc gcatccgatg
     2401 ggtaaatcga aaactcgacc gtgtgtccgc ttgcgcttgc ggccaagttg gcgtcgacga
     2461 tccgctcgtc cgcccagcat gaccggccca gcgcgtgggt gaaaatcccg atcagcttgc
     2521 cgagcaaaaa aggacagccc cagcggaacg gcgcattcgc ttgcgagcgc agctcccagc
     2581 gtttttcacc cgcaccacgg cggtgccatg tttcggttca aacgaaggca gctcgaagtg
     2641 tccccaacca gcgacaccga cggctttgcc ccatgccaga aagccttcag aaaacgtcgg
     2701 cccgagcttt tgcaccatga actcgtagtc ctctttggtg ccgcgactcg attcaagagc
     2761 gatgagcagt cgcgcaagct cggaccccgc ttcctgcaca agcggcgaca gcattcgata
     2821 cagcgacggg tcgagccaga acagcgccga cggaatgcca aagaagctct gcgtgccctc
     2881 ttcgagatcg gcctcgaaag gtacgctgcc aatggttgcc cgaaagactt tttccatcgc
     2941 agccgattat cgactgcgtg agacggagat ggaaccccga ttgagctgcg caggctcggt
     3001 ccgaccggtc gaatgtgcgg aagcttgcga cgtgccgctt acaaacgcga cagaagctgg
     3061 tccaggtagt gacccattcc gtagtcgagc cagcgtcgat atggctggtc ggcacaaatg
     3121 tagcccaggt ggccaccgtg cttttccatg tgcaggtgga ttcccggccc tagctgcgcc
     3181 tgtacaaagt cggtcggatc gatgaacgga tcatcttccg catgcaaaat caccgtcggt
     3241 cgcgtgatct tggataggtg tggcaaggcg ctcgaccgct cgtaatagtc ttgtccgctt
     3301 ttgaagccgc caagcggcgc cgtaaacacg tcgtcaaacg tcttgagcga catccgcagg
     3361 tggaccggga agcgtccttt ttcatgcaga ccatcggctt ctctctgccg gacgtagcgc
     3421 acacagccgc gcaaaaagta caggttgaac agtcggtggt tcggcgcgca gataaggtcc
     3481 gcgcagcgcg aaaggttgat gggcggattg accgcgatgg ctgcatcggg atggtggtgc
     3541 ggaccgccat gaccgtctcc cagacacaaa agcagggcgt tcgcgctcag ggaaaagccg
     3601 accccgatga tcgctgcgtc tggttgcttt ttgcgcacgg cggcaaacac ggcggacagg
     3661 tcactggcga caccgctgtg gtagatgcct ttggccagtc cgcgtccctg tccgcagccg
     3721 cgatgatcga cggaaaagac ctcgcagccg cgcgccgttc ccagatcgac cacccgctgc
     3781 atgtacggac ggtcgccgtg cccgcccagc ccgtggaaca cacagatcac cgtctttcga
     3841 tttggcggcg cagacccttc tttgggcgca tacacgcgcg caaccaagcg gtcgccatcg
     3901 ggcaggtgaa tttcttcgcg tcgccacggc tgggttagct tgggttttgg cagatagttg
     3961 ccgagcgccg actgaagata gccactcttt gcccacagcg gcggtcgaca cggcttttct
     4021 ggacgcggaa gctgcatgcg ctaattgttg cgctcgaccg tgtaattccc aagtgcgcgc
     4081 agcggctcgg cgtggacccc aaacggagcc agggccaaaa tgcctgcgtc catcacctgc
     4141 cgggcgtgcg ccatcgcgcc ttcgactccc agcagatcga catacgtggt cttgcctgcc
     4201 tctcggtccg aaccggggtc tttgccaagc tgggccagat ccgctgtcac gtcgagcacg
     4261 tcatcgacaa tctgaaatgc cagtcccagc gcctgcgcat aggtgcggat gtggccaagc
     4321 tgctcgtccg tcgcaccgcc gagccgggcc ccaccacgca gcgacacatc gaacagcgcc
     4381 ccggtcttca tcgcgtgcag cactttcagg tggtcgagat cgactttttt gccggtcgct
     4441 tcgacatcaa tcacctgccc accgaccatg ccaggggaac ccgcggcaaa cgacagctcg
     4501 cggatgattt cgaccagcac ggcagcaggt gccggactct gtgccgccag cgcaaacgca
     4561 tcggtcaaga gcgcgtcgcc ggccaagatt gccagcgcct cgccgtagac cttgtggttt
     4621 gttggacggc cccggcgaaa gtcgtcgttg tccatcgccg gcaggtcgtc gtgaatcagc
     4681 gaataggtgt ggatcatctc gaccgcacag ccaaagtgca gcgccatggt gggcggcttg
     4741 cccatcgctt cgcagccagc catcgccaag atcggtcgca gccgcttccc cccggcgagc
     4801 aggctgtagt tcatcgcttc cgcaagcttg cccggcgcaa accctgtgtc gccgtggaca
     4861 agtgctttca gtttgccgag ctttgccagc tctgcctcga cgagatcccg ccgagccgac
     4921 agataactct ttaggtcaaa agacatgggc gaagtttcta cagccaaagc ccgtatcgcg
     4981 gcacaaaaaa tgtggtcttc gcaaaagccg aacaaacttc gcaagctgtg gcctcgaaag
     5041 ccgtgcggac cgtgatagat tcgcgccgat atgaggcact tggagctgaa ggttggcacc
     5101 ctcatcgtgg tgtcgggcgg tctgctcgcg atgttcatcg cgatcctggg cggtttttcc
     5161 tttggcccga cgagtcggat ctacgtcgac tacgactttt ccggcaacat ccacgaaggc
     5221 gccccggtca aaatttccgg catcaaggtc ggcaaggtcg aaaagatcga atttttcggc
     5281 ggtgagctag acccgcaagt caatcgtcgg gtccaagtgc ggctgacggt gcagatcgag
     5341 gagcgcgcgc gcaaagccat ccacgacgac gccgagtttt tcgtcaacac ccaaggcgtg
     5401 ctcggtgagc agtacctaga aattgcgccg ggtcgtcacg aaaacaccca gcttccgccc
     5461 ggcagcaagg tcaaaggcgt cgatccgccc cgctcggacc ttatcatcgc ccgtctgtat
     5521 gagtttttgg atgcggtgac gcggcttctg caagaagaca aagaatcgct gcgcgatctg
     5581 ttgcgctcgg cggcgaaagt ggcgcgcagt ctcgacaagc tgctcggcga aaacgaagcc
     5641 gaggtcaaga agctgctcac cgacctggac cgcctgacgg cagagaccgc ggaactcgtc
     5701 gccaagctcg acaaaggcgt cggcacgggc gaggagctgc acaagacgct cgccaacatc
     5761 gagtcgatct cgacggaact aaagaccgag cttgcgccgc ttttgcaaaa gaccaagcgt
     5821 gccctcgatg gtgtggggga cgtggcacag cttcttggac cggaagaaaa gaaaaagctc
     5881 cagcgttcgc tcgacgatct gcttgtgctg tcgggcaagg tgtcgcaggt ggccggcgat
     5941 gcgcagtcgc tgctttcgga tgtccgccgc ggcaaaggca ccgtcggcgc actgattgtc
     6001 gaccagcaga tctacgacga cctcaaggaa ctgacccgcg acttaaagcg caatccttgg
     6061 aagtttttct ggaaggagta gcgcgtgagc ctcgggccac cgtttgacgc tggcaaccag
     6121 aaactgcacc tgctgctggg cggcgtgacg gtgctgcttt tgtcgttttt ggtggcggtg
     6181 gcgctgctta ccgccgatca ctcggtcggc aagtcggtca cgctgcatct tttggtccgt
     6241 cgcagcggcc agctttacac tggcgccgaa gtccacttgg ccggacagcg catcggtgag
     6301 ctgacagcca tccgtcgcta ccatcccagc gaacaagatc ccgctgagcg ccgggcccgc
     6361 ggtacgcagg tcgagctgga gctgcggatc ttgcgtgggc cggcgggtcg gctgtttcac
     6421 aactcgacgt ttgtggcgcg caatccgacg ctgctgtcgc ccgcagtcat cgaagtcggt
     6481 ccgcccgatc tggggcaaga caaaggaccg ccgattgcgg acggcgactt tttgtatggc
     6541 atcgacccgc ccgacctcga tcagctcctg cggcgcatct atgacagctt gacggtcatc
     6601 ttgaccgaag cgcagaccct gcggcccgag tggcaagaag cggcggcggc gtttgtcggg
     6661 ctgcagcgcc ggat
//
LOCUS       DAORGN010000244         6649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5449_length_6649_cov_4.660449, whole genome shotgun sequence.
ACCESSION   DAORGN010000244 DAORGN010000000
VERSION     DAORGN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_6649_cov_4.660449"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PKL17_19200"
     CDS             complement(<1..1026)
                     /locus_tag="PKL17_19200"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNK46920.1"
                     /translation="MQTLRLSGPTLSEPRIVRLQKPLTTIGRSSDNDIVVSDAQTKPS
                     HAYFSRGDGDVTVVAVDGDVLSGGKKKSKVKLQLGDKVQLGGLTIELIAEPVAAAVPK
                     ASGDADVLSALRKLHRFSERLLNHYEPEELWAALVDAVVEITQADNGFLVLCEPGMPP
                     RIKLGRAKGQLTVSEPDAKLSDSIVAKVLRTEQPVIVSDACKDAEFQNAQSVMHLRLT
                     SVMCVPLRERGQLLGALYVGTSDVRRLFRSADLESLTVIAAQAGLLLRNAHLLSELRR
                     DNQRLGVELEQMRSQAIASAGIGLGASQVMQDVMRTVAKVAQSDVSVLITGESGTGKE
                     LVAQEIHR"
     gene            complement(1185..2249)
                     /locus_tag="PKL17_19205"
     CDS             complement(1185..2249)
                     /locus_tag="PKL17_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNK46921.1"
                     /translation="MPASLSTLPTDYAAFLFDLKTRVRAAQLRAAVVANCEMVMLYWS
                     IGRDILDRQDRVGWGAKVIDRLSADLQSEFPTMRGLSVRNLKYMRAFAKAWPDPKIVQ
                     QAAAQLPWFHNCVLLDKLKSTEERLAYATAAIEHGWSRDILEIQIETGYLERLGKSVN
                     NFHRTLPKPQSDLANQTLKDPYLLDFVELSGDVSERRLERALVEKIRSFLLELGVGFA
                     FVGNQVHLEVDGEDFYLDMLFYHLRLRCYVVVELKATSFRPEYAGKLQFYLSAVDDLI
                     RDSQKDGPTIGLLLCKSKKRVIVEYTLREGKRPIGVANYQLSRSLPEDLAKNLPTVDA
                     LEAELSQHPIRMEEIEDTES"
     gene            complement(2314..3282)
                     /locus_tag="PKL17_19210"
     CDS             complement(2314..3282)
                     /locus_tag="PKL17_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46922.1"
                     /translation="MLRRSAVTLSCGFVILNGHTQLSHAEDLHPTPRREPPRLASPLP
                     SATDQSPEARLLRGKLAYQRGDYAAAVLTLRPLLYPQTLLSQEDQLLLAHKLLALSYF
                     FEHDEPGAEQEFNLLLSLRPDFALDPLVDPLKAVAFLDDIRRRNEQRLEEIRRRQSEE
                     EQRQKAEAEALQKRAEQLAQKQAKRLYFERVVSRKISPLQFVPFGVPQLADGRTAVGT
                     VLLVGEALTGAASAGAWLTVKLRYPDNTFPAREYTAAQALTGTYLGTGAAFWVLVLAG
                     LTDALWHARTAVEVRQLSSPPKELTPSQEAEATPPTPIAPAVSLAF"
     gene            complement(3394..>6649)
                     /locus_tag="PKL17_19215"
     CDS             complement(3394..>6649)
                     /locus_tag="PKL17_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK46923.1"
                     /translation="LVNKWFLNRAELPPPDDACQKPENRGEGKRLHDANGDGVFNVQD
                     YTTSKGHEQPDFAKRCDPRVSDTNKNGLLDPQDLIAAFSDGRDDDRNGYIDDIAGWDF
                     FRNDNDAYDDTHYGHGNGEAQDSSAETDNGIGDAGVCPRCRVMPLRVGDSFVADVNDF
                     AVAASYAVDIGASVIQEALGTLDNSALARWAIDYAYDNHVTVIASAADENSFHHNFPG
                     TNNHTVYVHAIRYNADRREDASHAFAFDNCTNYGAQLQLSVPGTHCSSEATGRASGMA
                     GLLYSAALQADLPAPGRLHPPGKDDAPAGEPANSRDRVRRLTAEEVRQIMIGTVDKFY
                     DPAENMNPLAYPTGPGFVRRFGYGRVNARTAVDAILAGAIPPQVEIESPEWFQVLGKR
                     DGNVPIKGRIALRNGGVGAGKDQFDYVVEWAPGVDPKEGEWKKLAGAELQSQSLEGPL
                     GQLPAADLKIQNPQLSPDDPSWQPDDAAHVHALTVRVRATQRSADPLRNGLVGEARRT
                     VHIDSDPDLLPGFPLRIGASGEASLKMADLDGDGKRELIVADSSGLVHALRADGRELS
                     GWPVELPPIAALAKAPGGHLDAPAWSQPPSRPRPAFGQAVLATPAVGDVNGDGKLDVV
                     VASYDGTVMAIGPDGKVLPGFPVKVDGVARSQGTDPRKIIDDGFFAAPVLADLDGDKK
                     LDIISASFDGQVYVWKGDGARMPGWPQLIFDPQRPDDPLDPEPRQRARILSTPAVGDL
                     NGDGIPDLALSTNEQYGEYGRVYAIDGRGGKAAKLILPGFPVAVNNKDVLPVVGIGIP
                     NAPAMGDIDGDGVPELFFNAIAARMTVLKADGSLYKVALSSNKPAFGEKSNTREFSTL
                     SFIASPALGDLDGDGKFEAILPTSGANAALSMVKAYTRLDYEMNVSAWDLETGKMKPG
                     FPQAIEDYVFFMNPLVVDVDGDGKKDVVAGSAGYFVHAWNVDGQEARGFPKLAGGWIA
                     ATPTVGDMDGDGKLELATMTRNGWLFVWHVRGLSKGRMDWDSFHHDLRNTGNLATKLD
                     QGGDEVVPPDADPMEKATGGCDCQIGHKRASWLSLLLTALAACTLLFRRHLRRR"
BASE COUNT         1289 a         2151 c         1901 g         1308 t
ORIGIN      
        1 acggtgaatc tcttgcgcga ccagttcctt gccggttccg ctttctcccg tgatgagcac
       61 cgagacatca ctttgcgcga ctttggcgac ggtgcgcatg acatcttgca tcacctgcga
      121 tgcaccaagt ccgattcccg cactcgcaat cgcttggctg cgcatctgtt ccagttcgac
      181 gcccagccgc tggttgtcac gccgcagctc cgacaaaaga tgggcattgc gcagaagcag
      241 tccggcctgc gccgcaatca cggtcaggga ttccagatca gcactccgga acaggcgacg
      301 cacgtcgctg gtgccaacat acagagcccc caaaagttga ccgcgttcgc gcaaaggcac
      361 gcacatcacg ctggtcagcc gcagatgcat caccgactgc gcattttgaa actcagcgtc
      421 tttgcaggca tcgctgacaa tcaccggctg ctccgtgcgc agcactttgg ctacgatcga
      481 atccgacagt ttggcgtcgg gctcgctgac cgtaagctgt cctttggcac gtccgagctt
      541 gatccgcggc ggcattcccg gttcgcacag caccaaaaag ccgttgtccg cttgagtgat
      601 ctcgacgacg gcatcgacca gcgccgccca cagctcctcg ggttcatagt gattgagcag
      661 ccgttcggaa aagcgatgca gcttgcgcag cgccgaaagc acatcggcat ccccgctcgc
      721 tttcggcaca gccgccgcga cgggctcggc gatcagctcg atggtgagtc cgccaagctg
      781 aactttgtcg ccgagctgga gcttcacttt gctctttttt ttgccgccgc tcagcacatc
      841 gccatcgacc gccaccaccg tcacatcccc gtcgccgcgc gaaaaatagg cgtggctcgg
      901 ctttgtctgc gcgtcggaaa ccacgatgtc gttgtcgctt gatcgaccaa tggtcgtcag
      961 cggtttttgc agccgcacga tccgtggctc agacagcgtc ggacccgaca agcgaagagt
     1021 ctgcatgcgc ccctagaatg ccaaacttac cggaaaagga tagtcccccg tcgagatgac
     1081 gatttcccgc gcggtccacg agcaggtccg ccgtactcca gcttcatcgg aaatccgtgt
     1141 ggaccaccgc tagattcgga caacggcggg caatccaaac caggttacga ctcggtgtcc
     1201 tcgatctctt ccatgcggat cggatgttgg ctcagctcgg cttccagcgc atcaacggta
     1261 ggcagattct tggcaagatc ctcgggcagc gagcgggaga gctgatagtt ggcgacgccg
     1321 atggggcgct tgccctcgcg caacgtgtac tcgacgataa cccgcttctt gctcttgcat
     1381 aggagcaggc caatggtagg cccgtctttc tgcgagtcac ggatcaggtc atcgacagcg
     1441 gacaaataga actggagctt gcctgcgtac tcgggccgaa atgacgtcgc cttcagctcg
     1501 acgacgacat agcaacggag acgcaggtga tagaaaagca tgtcgaggta aaagtcctcg
     1561 ccatcgacct ccagatggac ttgattgccg acgaaagcga agcccacgcc cagctccaat
     1621 aggaagctgc ggattttctc gaccagcgcg cgctccagtc gacgctcgct cacatcgccg
     1681 ctcagttcga cgaaatcaag caggtacgga tctttcagcg tctggttggc aaggtctgac
     1741 tgcggttttg gcaaggtccg atggaagttg ttgactgact tgccgagacg ctcaaggtag
     1801 cccgtttcta tctggatttc gaggatgtcg cgcgaccagc cgtgttcaat cgcagccgtc
     1861 gcgtaggcca gccgctcctc cgtagacttg agcttgtcta gcaacacgca gttatggaac
     1921 cacggcaatt gtgcagcagc ctgctgcaca attttcgggt cgggccaggc cttggcgaac
     1981 gcccgcatgt atttcagatt gcgtaccgac aaaccccgca tcgttggaaa ctcactctgc
     2041 agatctgccg acagtcggtc tatcaccttc gccccccagc caacacggtc ctgccggtcg
     2101 aggatgtcgc gcccgatgct ccagtagagc atcaccatct cgcagttggc gacgacggca
     2161 gcccgaagct gtgcagcgcg tactcgtgtc ttcaggtcaa aaagaaaagc agcgtagtct
     2221 gtcggaaggg tcgagagcga cgcaggcatg ggcggacgtt acaacacccg tgaaagccgg
     2281 gcatgcattc ttcctcgaac ccagcggccc aggtcaaaat gccaaagaga ctgcgggggc
     2341 gataggtgtt ggtggggtgg cttcggcttc ttgggatggg gttagctctt tgggcggcga
     2401 cgacagctgg cggacttcga cggcggtccg ggcatgccac agcgcgtcgg tgagccccgc
     2461 cagcaccagc acccaaaacg ccgcgcccgt gcccaaatac gtccccgtca gcgcttgcgc
     2521 cgccgtgtat tcgcgcgctg gaaacgtgtt gtcgggatag cgcagcttca ctgtcagcca
     2581 cgctccagcc gacgccgccc cggtcagcgc ttcgccaacc agcagcaccg ttcccaccgc
     2641 ggttcgtcca tcggcgagct gcggcacccc aaacggcaca aactggagcg gactgatttt
     2701 gcgcgacacc acccgctcga aatacagacg cttggcctgc ttttgcgcaa gctgctcggc
     2761 gcgtttttgc agcgcctcgg cttcggcttt ctgccgctgt tcttcttcgc tttgccgacg
     2821 acggatttct tctagccgct gctcattgcg gcgccgaatg tcgtcgagaa atgccaccgc
     2881 tttgagcgga tcgacgagcg gatcgagcgc aaagtcgggc cgcagcgaca aaagcaggtt
     2941 gaactcttgt tccgcccccg gctcgtcgtg ttcaaaaaaa tacgacagcg ccaaaagctt
     3001 gtgggcaagc agcagctgat cttcttgcga aagcagcgtc tgtggataca aaaggggccg
     3061 cagcgtcagc accgccgccg catagtcgcc acgctgatag gccagctttc cccgcaagag
     3121 cctcgcctcg ggactctgat cggttgcact cggcagcgga ctggcaagcc ggggtggttc
     3181 tcgtcgcggc gtcggatgaa gatcctcggc atgcgacagc tgcgtgtgac cgtttaggat
     3241 cacaaagccg cagcttaggg tcacagccga ccgtcgcaac atctatgcaa ggtcatatca
     3301 cggagccacg gcggcccgga caaaaagtga gcgcacacaa gccgcgtcgc agcgtccacg
     3361 cgggtggaat cagcagggga gatcagcagg ggatcagcgg cgacgcaggt gacgacggaa
     3421 cagcagcgtg caggcggcca gcgccgtaag caaaagcgac agccaggacg cgcgcttgtg
     3481 accgatttgg caatcacaac cacctgtcgc cttttccatc ggatcagcgt cgggcggcac
     3541 cacttcatca ccgccttgat ccagcttggt cgccagattg ccggtgttgc gcagatcgtg
     3601 atggaagctg tcccagtcca tccggccttt gctcagtccg cgcacatgcc agacaaacag
     3661 ccagccgttg cgagtcatcg tcgccagttc aagcttgcca tcgccatcca tgtcaccgac
     3721 ggtgggcgtc gccgcaatcc agccgccagc cagcttggga aagccgcgtg cctcttgccc
     3781 gtcgacattc caagcgtgga caaaataccc cgccgatccc gcgaccacat cctttttgcc
     3841 gtcaccatcc acatcgacga cgagcggatt cataaagaac acgtagtctt cgatggcctg
     3901 cggaaagccg ggcttcatct tgccggtttc caagtcccac gccgagacgt tcatttcgta
     3961 gtcgagtcgc gtatacgctt tcaccatcga cagcgccgca ttcgcacccg aggttggcaa
     4021 aatcgcttca aacttgccat cgccgtcgag atccccgagc gccggactgg caatgaagct
     4081 cagcgtcgaa aactcgcgcg tgtttgactt ttccccaaac gccggcttgt tcgatgacag
     4141 cgcgactttg tacagactcc catcggcttt caacaccgtc atccgcgccg cgatggcatt
     4201 gaaaaacagt tccggaacgc cgtctccgtc gatgtcaccc attgccggcg cgttgggaat
     4261 gccgattccg acgaccggaa gcacgtcctt gttgttcact gcgacgggga agcccggcag
     4321 aatcagcttg gccgctttgc ctccgcgtcc gtcgatggcg tagacccgtc cgtactctcc
     4381 gtactgttcg ttcgtcgaca gcgccagatc aggaatgccg tcgccgttca gatcgcccac
     4441 cgccggcgtc gacaaaatcc gcgcccgctg ccgcggttcc ggatcaagtg gatcgtcggg
     4501 acgctgcggg tcaaaaatca gctgcggcca gccaggcatc ctcgcgccat cgcctttcca
     4561 gacatagacc tgaccgtcaa acgacgccga aatgatgtcg agctttttgt ccccgtcgag
     4621 atccgccagc accggcgcgg caaagaaacc atcgtcgatg atcttgcgcg ggtcggtgcc
     4681 ttggctgcga gcgacgccat cgactttgac gggaaagccg ggcaggactt ttccgtcggg
     4741 accaattgcc atcaccgttc cgtcgtaact ggccaccacc acatccagtt ttccgtcgcc
     4801 atttacatca cccaccgcag gtgtcgccaa caccgcttgg ccaaaagccg gacgaggacg
     4861 cgacggcggc tggctccatg cgggtgcatc caagtgaccg cctggcgctt tcgcaagcgc
     4921 cgcaatcggt ggcaattcga ccggccaacc agaaagctcg cgtccgtcgg cacgcagcgc
     4981 atgaaccaga ccgctcgaat cggccacaat cagctcgcgc ttgccgtctc cgtcgagatc
     5041 cgccatcttc agcgaggctt cccccgacgc cccaatccgc agcggaaacc ccggcagaag
     5101 gtccgggtca ctgtctatgt ggaccgtgcg tcgcgcttcc ccgacgagcc cattgcgcag
     5161 cgggtccgcc gatcgctgcg ttgcccgcac ccgcaccgtc agcgcatgga cgtgggccgc
     5221 atcgtcgggc tgccagcttg gatcatctgg cgaaagctgc ggattttgaa tcttgaggtc
     5281 cgccgccgga agctgcccaa gcggtccttc gaggctctgc gactgcagct cggcaccagc
     5341 gagctttttc cattccccct ctttgggatc gacaccgggc gcccactcga cgacgtaatc
     5401 gaactgatct ttgcctgcgc cgacgccgcc gttgcgcagc gcgatgcgac ctttgatggg
     5461 gacgttgccg tcgcgcttgc ccaacacttg gaaccattcg ggactctcga tttcgacctg
     5521 cggcggaatc gcgcctgcca aaatcgcatc gacggcggtt cgggcattga cgcgaccgta
     5581 gccaaagcgc cgcacaaagc caggccccgt cggatacgcc agcgggttca tgttttcggc
     5641 gggatcatag aacttgtcga cggtgccgat catgatctgc cgcacttctt ctgccgtcag
     5701 tcgtcgcacc cgatcgcgcg aattggccgg ctcgcccgcg ggcgcatcgt ctttccctgg
     5761 tggatgcagt cgccctggcg ccggtagatc cgcctgcagc gccgcactgt acagcagccc
     5821 cgccattccc gaagcccgac ccgtcgcttc tgatgagcag tgcgtgcccg gcaccgagag
     5881 ctgcagctgc gcgccgtagt ttgtgcagtt gtcgaacgcg aatgcatgac tggcatcctc
     5941 gcgtcggtcg gcgttgtaac gaatcgcgtg cacatacacc gtgtgattgt ttgtgcccgg
     6001 aaagttgtgg tggaagctgt tttcgtcggc agccgaagca atcaccgtca catggttgtc
     6061 gtaagcgtag tcaatcgccc agcgagccag cgccgagttg tcgagcgtac cgagcgcttc
     6121 ctgaatcacc gatgcgccaa tgtcgaccgc atacgacgct gccaccgcaa agtcattgac
     6181 gtcggccaca aagctatcgc caacccgcag cggcatcacc cgacagcgcg gacagacacc
     6241 cgcatcgccg attccgttgt cggtttctgc ggatgaatcc tgcgcttcgc cattgccgtg
     6301 tccatagtgc gtgtcgtcat aagcgtcgtt gtcattgcgg aaaaaatccc agcccgcaat
     6361 gtcatcgata tagccatttc gatcatcgtc gcgcccgtcg gaaaatgccg cgatcagatc
     6421 ctgcggatcg agcagtccgt ttttgtttgt gtcactgacc cgcggatcac agcgcttggc
     6481 aaaatcgggc tgctcgtggc ctttgctggt tgtgtagtcc tggacgttga aaacgccgtc
     6541 gccgttggca tcgtgcaggc gcttgccttc gccgcggttt tccggtttct gacaagcgtc
     6601 atccgggggc ggcagctcgg cccggtttag gaaccacttg ttcaccaga
//
LOCUS       DAORGN010000245         6620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5481_length_6620_cov_4.459254, whole genome shotgun sequence.
ACCESSION   DAORGN010000245 DAORGN010000000
VERSION     DAORGN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5481_length_6620_cov_4.459254"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKL17_19220"
     CDS             complement(<1..522)
                     /locus_tag="PKL17_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46924.1"
                     /translation="MEHSVSPSTKPPTGSARGPADVSLGSPSLPPPSSSSSLTKQIVI
                     ASVVSIVALAIVCGLGLSSLRRLSLQNDAEMAQRLALLDESEVFQLLLYQKGFAVDFM
                     FTGDERWLRELSEGRREFQDWLLQAKRNADSIEERQLLDRIGSDYQRLDEAHAAMIAG
                     FRQQKQDQVRQQHQ"
     gene            646..1503
                     /locus_tag="PKL17_19225"
     CDS             646..1503
                     /locus_tag="PKL17_19225"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNK46925.1"
                     /translation="MKSIRSFAALSTLSLLAACGGPTQATVPDDAQKAAAAAKDDSCT
                     APKDCYNKAALALQAKDNAKAMKFLPGACDHDTKACVIAAEILANGQGVPADRPKSAD
                     FYKRGCDGGILVACANEASMRYTGEGGVTVDKTRARALFEKTCGPEMLDNCNNAATMM
                     ASGDGGPEDKTKARSLYDMACGGAYLQACVAGGAMYLAGQGGGEDKAKAKSYFEKACD
                     GKNADGCFNLGVLYGSGNGVSKDIDKAQQYFQKGCELGNKKSCETLDQLKADIEKSKA
                     PAPKAKAKK"
     assembly_gap    1688..1787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1788..2865
                     /locus_tag="PKL17_19230"
     CDS             <1788..2865
                     /locus_tag="PKL17_19230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK46926.1"
                     /translation="PVAIVHADPIRFSLKTPVPQPDKPQLVVIADQPISDLSVAVLAK
                     EPDPALGSASGESGEFKYQAKKLSAGQKMSFGLGSGKPGATHWEGMISAKVGGAPWKQ
                     PVTFDTVLTRTIQIGYDKNYVSPHLNVEKRFVELTLSSPAGHAEIEVFSDEGIKMGEG
                     KASFAGEPAGTWLRIPWDGTSAAPNESVVLRLSVKLFDKDGASGHINLYPWFVSVPHE
                     DVNFETNSWEVRDSEKLKLDESYKRISAVLDRVEKTLLKWAEQGVVLGSLPQPQLFVT
                     GHTDTVGGDNDNVVLSRNRAKAIATYFRQKGFRMPIYFVGYGERTPRVHTGDNVDEIR
                     NRRADYQMMLQPPPTLTGTGWTKL"
     gene            3006..3191
                     /locus_tag="PKL17_19235"
     CDS             3006..3191
                     /locus_tag="PKL17_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46927.1"
                     /translation="MKKLILGTLCAFALNAAGLSLASAEDKPADAKAEKAEKPAKKGG
                     KKKAEAKKDEAPKAETK"
     gene            complement(3219..5057)
                     /locus_tag="PKL17_19240"
     CDS             complement(3219..5057)
                     /locus_tag="PKL17_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNK46928.1"
                     /translation="MQTAESRDLVLDRLWQHARARPSEPVLYQRKSDGEWGHITFAEY
                     RDQVHRVACACVAAGMPEGGSVGIFCENRAEWVLCALGAQAAGGMASGIYMNSTPDQV
                     AYILAHSEATLLVVEGPRQVESLLPHLPSLPKLRKIVMLTEADASRPLHTRIDLVTSW
                     QAFLDSGKGHEPEVDERFARLSPDRIATLIYTSGTTGGPKGVMLTHHNLAWTARAGLS
                     AYQVDTQDVMVSYLPLSHIAEQMLTVYIGITAGVKVYFAGGIDNLRDTLLAARPTVLF
                     GVPRVWEKLQAALETKLAAQKPIQRRVIAWARDVGSRVGHYRIESGSPFGLLALEEIV
                     ARKLFFTKIRSQLGLDRLRFAVSGAAAIRKDVLEFFLSLSIPIHEVYGLSECSGPLTL
                     NTAQPGQTRLGTTGRPLPGTALKLAPDGEILFRGPNVALGYYKDPTATEQTFQDGWLY
                     TGDVGEIDEAGFLRITDRKKDLLRTSGGKYIAPQPIESLLRAMPLVSQAVVICEGQKY
                     ATCLLTLDPERAGKFAKEHQLPTDMAELGRHEKVHALMAESIERVNQSLARYEQLKHF
                     TILPRDFSIEREEVTPTQKLRRKAITKNYAPEIAAMYADSEGAEKG"
     gene            5236..>6620
                     /locus_tag="PKL17_19245"
     CDS             5236..>6620
                     /locus_tag="PKL17_19245"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNK46929.1"
                     /translation="MKIGIIKEIKPGERRVAATPETVGKLKKLGFDVVVESGAGEGAS
                     FRDQDYEQAGAHIEKDTAQMWGQCDIVLKVQPPDMHPQLGRHEAELLREKATLVSFLW
                     PAKNRELIEKLATRKATVLAMDLIPRITRAQKMDALSSMANIAGYRAVIEAASFFGRF
                     FTGQVTAAGKVPPAKVMVIGAGVAGLAAIGAARGLGAIVRAFDTRPVVREQVQSMGAE
                     FLEVELAEDGTGEGGYAKVMSPEFIAAEMALFEKQAREVDIIITTALIPGKAAPQLIL
                     RSHVEQMRPGSVIVDMAAESGGNCEVTRPGEVINHRGVTVIGYVDLPSRLAPTASSLY
                     GNNLVHLLTDMGGAAKWQVNQQDEVVRGALVVCDGEILGPPPKPNAPPPDQQKKPAAA
                     VAKPVAKEAKGGHDDTSISPKAQVTALLAMAAAFLVIGLFAPPAFLAHFTVFVLACFV
                     GWQVVWNVTPAL"
BASE COUNT         1367 a         2009 c         1975 g         1169 t
ORIGIN      
        1 ctgatgctgc tgccgcacct gatcctgttt ttgctgacgg aagccagcga tcatcgctgc
       61 gtgcgcttca tccagccgct ggtagtcgct gccgattcgg tcgagaagct gccgttcttc
      121 gatgctgtcg gcattgcgct tggcctggag cagccagtct tggaactcgc gccgcccttc
      181 gctcagctca cgcagccagc gctcgtcccc ggtgaacatg aaatcgacgg caaagccctt
      241 ttgataaagc aggagctgga agacctcgct ttcgtcgagc agcgccagcc gttgcgccat
      301 ttcggcatcg ttttgcaggg acagccgccg cagcgagcta agcccgagcc cgcagacaat
      361 cgccagcgca acgatcgaca ccaccgacgc aatgacaatc tgtttggtca gtgagctaga
      421 agacgacggc ggcggcagac tgggcgatcc gagcgaaacg tcagcaggac cgcgcgcact
      481 gccagtcggt ggtttggtgg atggggaaac agagtgctcc atggcaaggc cagcgttttt
      541 cacttgtaca tgaggagggc tcgacgcgat tgacaaaagt gccccaccgg tcgtacatcg
      601 cgctgcagaa gtgtttgttc caacctggat acgaggaaac tgaagatgaa gagcattcgt
      661 tcgtttgctg cgctttccac cctgtcgctg cttgccgcct gtggcggacc gacccaagcg
      721 acggtccccg acgacgccca aaaggcagcc gcggcggcca aggacgattc ctgcaccgcg
      781 cccaaggact gctacaacaa ggcggcgctc gcgctccaag ccaaagacaa cgccaaggcg
      841 atgaaatttc tgcccggcgc ctgtgatcac gacaccaaag cgtgcgtcat cgcggcagaa
      901 atccttgcca atggtcaggg cgtcccggcg gatcgtccca agtcggctga cttctataag
      961 cgcggctgtg acggcggcat tttggtcgct tgcgccaacg aagccagcat gcgctacacc
     1021 ggggaaggcg gcgtcaccgt cgacaagacc cgcgcccgcg cgctgtttga aaagacctgc
     1081 ggccccgaaa tgctcgacaa ctgcaacaac gcagcgacga tgatggcgtc gggcgatggc
     1141 ggaccggaag acaagaccaa agcgcgcagc ctgtatgaca tggcgtgcgg tggtgcctac
     1201 ctgcaggcct gcgtcgcggg cggcgcgatg tatctggccg gccagggcgg cggcgaagac
     1261 aaggcgaaag ccaagagcta ttttgaaaaa gcctgcgacg gcaaaaacgc cgatggctgc
     1321 ttcaacctgg gcgtgctgta cggcagcggc aacggcgtca gcaaagacat cgacaaagcc
     1381 cagcagtact tccaaaaagg ctgcgagctg ggcaacaaga agtcgtgcga gacgctggat
     1441 cagctgaagg ccgacatcga aaagtcgaaa gcgccagcgc ccaaagccaa agccaagaag
     1501 tagtccccga cggccccgcc ccgccctccc tgccgcagat ccccccatca cgacctcgtg
     1561 atgcttgttc acgtcttcgt gatgcttgtt cacgacctcg tgatgtttgt tcacgacctc
     1621 gtgacgccgc catcacgacc tcgtgatgct tgttcacgac ctcgtgatgc cgccttcacg
     1681 acctcgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc tgtggcgatc
     1801 gtccacgctg atccgattcg cttttcgctg aaaacgccgg tgccgcagcc cgacaaaccg
     1861 cagcttgtgg tcatcgccga ccagccgatt tcggatctgt cggtggcggt tttggccaaa
     1921 gaacccgatc cggcgctcgg cagcgcgtcg ggggaaagcg gcgagtttaa gtaccaagcc
     1981 aaaaagctct cggcggggca aaaaatgtcg tttgggctcg gttcgggcaa gccgggcgcg
     2041 acgcactggg aaggcatgat cagcgccaaa gtcggcggtg cgccgtggaa gcagccggtg
     2101 acgttcgaca cggtgctgac gcggacgatc cagattggct atgacaaaaa ctacgtctcg
     2161 ccgcacctga atgtggaaaa gcgcttcgtc gagctgacgc tatcgagtcc cgccgggcac
     2221 gccgagatcg aggtcttttc cgacgaaggc atcaaaatgg gcgaaggcaa agcgagcttc
     2281 gctggggaac ccgccggcac ttggctgcgc atcccgtggg acggcacgtc ggcggcaccc
     2341 aatgaaagcg tcgtgctgcg gctgtcggtc aagctgttcg acaaagacgg tgccagcggc
     2401 cacatcaacc tgtacccgtg gtttgtttcg gtcccgcacg aagacgtaaa ttttgaaacc
     2461 aactcatggg aagtgcgcga cagcgaaaag ctcaagctcg acgaaagcta caagcgaatc
     2521 agcgccgtgc tcgatcgggt cgaaaaaacg ctgctcaaat gggccgaaca aggcgtggtg
     2581 ctgggctcgc tgccgcagcc acagctgttc gtgaccggac acaccgatac cgttggtggc
     2641 gacaacgaca acgtggtctt gtcgcgcaac cgagccaagg cgatcgccac gtatttccgt
     2701 cagaaaggct tccgcatgcc gatttatttc gtcggctatg gggaacgcac gccgcgggtt
     2761 cacaccggcg acaacgtcga cgaaatccgc aaccgccgcg ccgactacca aatgatgctg
     2821 cagccgcccc cgactctcac cggcacgggc tggaccaagc tgtaagcgga cgcgattcag
     2881 acctgggggg gacagtcccc cacttgtagc gcttgcacat tgaaacgact tggtagatgt
     2941 tccgcaaaag ctgacgcgct gaaactacag cgcaggcttt ttacccccca aaaggagtct
     3001 tggtcatgaa gaagctgatc ctcggaacgc tgtgtgcatt cgctctcaac gctgctggcc
     3061 tgagcctcgc ttcggcggaa gacaagccgg cggatgccaa ggctgaaaag gccgaaaagc
     3121 ccgccaagaa gggcggcaaa aagaaggccg aagccaagaa ggacgaagcc cccaaggctg
     3181 aaaccaagta gtccaaagcc ggggcagctt gttctgccct agcctttttc tgcgccctcg
     3241 gaatccgcgt acatcgcggc gatttcgggg gcgtagtttt ttgtgatggc tttgcggcgc
     3301 agcttttggg tcggcgtgac ttcttcgcgc tcgatcgaaa agtcgcgcgg caaaatcgta
     3361 aagtgcttga gctgctcgta acgggccagc gactggttga cccgttcgat gctttcggcc
     3421 atcagcgcgt gaactttttc gtgtcgccca agttccgcca tgtccgtcgg aagctgatgc
     3481 tctttggcaa acttgccggc ccgctctgga tcaagcgtca gaagacaggt tgcgtacttc
     3541 tgaccttcac agatgaccac ggcctgcgac acgagcggca tcgcacgaag cagggattca
     3601 atcggctgcg gcgcgatgta tttgccgccc gacgtgcgca gcagatcctt tttgcgatcg
     3661 gtgatgcgca gaaagccggc ttcgtcgatt tcgccgacat cgccggtata cagccagcca
     3721 tcttgaaacg tctgttccgt cgcagtcgga tctttgtagt agccaagggc gacgttcgga
     3781 ccgcgaaaca gaatctcacc gtctggggcc agcttgagcg cggtgcccgg cagcggtcgt
     3841 cccgtcgtcc ccagtcgtgt ctgtcctggc tgcgccgtgt tgagcgtcag cggccccgaa
     3901 cactcggaca agccatagac ttcatggatc gggatgctca gcgacaggaa aaattccagc
     3961 acatctttgc gaatcgccgc cgcgcccgac accgcaaagc gcagtcgatc gagtccaagc
     4021 tgcgagcgaa ttttggtgaa aaacagcttg cgggcgacga tttcttccag cgcgagcaga
     4081 ccaaacggcg atcccgattc gatgcggtag tgaccgaccc ggctgccgac atcacgggcc
     4141 caagcaatca cgcgacgctg aatcggtttt tgcgcggcga gcttggtttc gagcgcggct
     4201 tggagttttt cccacacccg cggcacacca aacagcaccg tcggacgtgc cgccaaaagc
     4261 gtatcgcgca gattgtcgat tccgccggca aagtagactt tgaccccggc ggtgatgccg
     4321 atgtagacgg tcagcatctg ctcggcaatg tgcgaaagcg gcagatacga caccatcaca
     4381 tcttgggtgt cgacttgata cgccgacaga ccagcccgcg ccgtccacgc caggttgtgg
     4441 tgggtcagca tcacgccttt gggcccgccc gtcgtccccg acgtgtagat aagcgttgca
     4501 atccgatccg gcgacagccg ggcaaagcgc tcgtcgactt ccggttcgtg gcctttgccg
     4561 ctatccagaa acgcttgcca gctggtgacc aaatcaatgc gggtgtggag cggacgactg
     4621 gcatcggctt ccgtcagcat cacgatcttg cgtagcttgg gcaggctcgg cagatgcggc
     4681 aaaagcgact cgacttggcg cggaccttcg acgaccagca gcgtcgcttc actgtgcgcc
     4741 aggatgtatg cgacttgatc cggcgtcgag ttcatataga tccccgacgc catcccgccc
     4801 gctgcttgcg cgcccagtgc gcaaagcacc cactcggccc ggttttcaca gaagatgccg
     4861 acggagccgc cttcgggcat ccccgcagcg acgcaagcgc aggcgacgcg gtgcacctgg
     4921 tcgcgatact cggcaaacgt gatgtgcccc cactctccat cgcttttgcg ctgatagaga
     4981 acgggctcgc tcggacgggc gcgggcgtgc tgccacagcc gatccaagac gaggtcgcga
     5041 ctctccgctg tctgcatggc gccatgctag ccacttccca gtcggcagca caacttccga
     5101 atgcgagtcc ggacgaaaaa ccacccggcg ccaatcggtc gaacaattga cccactgcct
     5161 gcttgtttct cgccgacgga tcggcctatt ctctttgcca aatctggggc ggacctgtcc
     5221 cgggcagcca aaaacatgaa aatcggcatc atcaaagaga tcaaaccggg agagcgccgc
     5281 gtcgccgcca ctcccgagac tgtcggcaag ctcaagaagt tgggctttga cgtcgtggtc
     5341 gagtccggcg cgggcgaagg cgcgtcgttt cgcgaccaag attatgagca ggccggtgcc
     5401 cacattgaaa aggacaccgc gcagatgtgg ggacagtgcg acatcgtcct caaagtgcag
     5461 ccgccggaca tgcacccgca gctcggtcgt cacgaagcgg agcttctgcg cgaaaaagcg
     5521 acgctggtga gcttcctgtg gccagccaaa aaccgcgagc tgattgaaaa gctggcgacg
     5581 cgcaaagcga cggtgctggc gatggatctg atcccgcgca tcacccgagc gcagaaaatg
     5641 gatgcgctta gctcgatggc caacatcgcc ggctatcgcg cggtcatcga agccgccagc
     5701 ttctttggtc gcttctttac cggacaagtc accgccgctg gcaaagttcc gcccgcgaaa
     5761 gtgatggtca tcggtgccgg tgttgccggc ctggccgcca tcggtgcagc gcgcggtctt
     5821 ggcgccatcg tgcgcgcgtt tgacacccgc ccggtcgtcc gcgaacaagt ccagagcatg
     5881 ggcgccgagt tcctggaggt cgagctggcc gaagatggca ccggcgaagg cggctacgcc
     5941 aaagtgatga gcccggaatt tatcgccgcc gaaatggctc tgtttgaaaa gcaggcgcgc
     6001 gaagtcgaca tcatcatcac gacggcgctg attcccggca aagcggcacc gcagcttatc
     6061 ctgcgcagcc acgtcgagca gatgcgaccg ggcagcgtca tcgtcgacat ggcagccgaa
     6121 tcgggcggca actgcgaagt gacgcgccct ggcgaagtca tcaaccaccg cggcgtgacc
     6181 gtgattggat atgtcgattt gccaagccga ctcgcaccga cggcaagcag cctgtacggc
     6241 aacaacttgg tccacctttt gaccgacatg ggtggggccg ccaagtggca ggtcaatcag
     6301 caagacgaag tggtccgcgg cgcgctggtg gtctgcgatg gcgaaatcct gggaccgccg
     6361 ccaaagccga acgccccgcc accggatcag cagaaaaaac cggctgcggc tgtcgcaaag
     6421 cccgtcgcca aagaagccaa gggtggacac gacgacacat cgataagccc caaagcacaa
     6481 gtcacggcgc ttttggcgat ggcggcagcg tttttggtca tcggactgtt tgcgccgccg
     6541 gcctttttgg cccacttcac ggtgttcgtg ctggcctgtt tcgtcggctg gcaggtggtc
     6601 tggaacgtca ctccggcact
//
LOCUS       DAORGN010000246         6591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5514_length_6591_cov_4.797277, whole genome shotgun sequence.
ACCESSION   DAORGN010000246 DAORGN010000000
VERSION     DAORGN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5514_length_6591_cov_4.797277"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="PKL17_19250"
     CDS             complement(<1..1997)
                     /locus_tag="PKL17_19250"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNK46930.1"
                     /translation="MRVRPTPHRPRLLQLIVASVLWAVGSMLPNVHAAPKLRFQTDVK
                     GDIVLIGNTLGQECRAVDSLGMSIPKPVVGTVGNCGDPITIDDSAADVLWRADDSTAK
                     ADGQTMIGDARSTSMLSLPQGSQVLYARLYWSGNLGEDIALANSTVSFEKVGPGGFSM
                     MLLPNPATDVVTAVGGGGGHVYQGSYDVTPIVQRYGTGAYRIGNVVRRNVVNRDEDVQ
                     FNAWTMLVVYRNDKEPVRNITIYDGLDSVDIGRSVNLGVIGFRVPEGGVPQGKLGVIA
                     YEGDSDKKDSFLFNNKPVTDGINPADNIFNSTRSTLGAAVSVTGDLPQLTGTAGSMSG
                     VDLDQIDVSSLLKANDTQATIQANSIDDVYFVGGLFTSIRSRKPVIETTLMADPSSVR
                     PGDTVTFTSTSKNVGDDDGTDVVIRHPLPDGMTYVPGSIVFVSGPEAGQNGPKSDTPG
                     DDQAEVINDPMTGKPVLVIRIGKGATANSGGRLSPSDSPVVVQYKLKTDPNTTLSKIP
                     TQSTTTATTAGNPALPPATFPSGNGEQPAAPTVVNLPNTQADLRVIVSKNPPFPQPGD
                     PVTYSIDVKNVGNSGDPGPLHVTFKVPPGGVIDSVTPGPGWTCTQQGDTFTCTRPDLP
                     QGTAPPITVKVMLPAGTTGGTPAHVGQVGSPGVDDPNLVNNR"
     gene            2164..2787
                     /locus_tag="PKL17_19255"
     CDS             2164..2787
                     /locus_tag="PKL17_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46931.1"
                     /translation="MRIWTTRCWVLLALLSLVLELGQAPRSLGAWWGESALMPRNPSR
                     NASRSLLLAKAESASADEKDDAADPALDATRGDDSGAGSGGGKEPDKDGHSDRDSDAT
                     ADDDTGSESDSDADEGGDSDPKSADESDEKPDGSSSEQREETERKAGEENDEENSGQK
                     DDPLSLLRAAAVAFSCDLTGQELRFGNTTRLCDLTTSDALLRPPRSV"
     gene            2910..4997
                     /locus_tag="PKL17_19260"
     CDS             2910..4997
                     /locus_tag="PKL17_19260"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNK46932.1"
                     /translation="MKRLYDRPYAGRHKDSPPVLAARIGSMWSAWLADWKDAIYEPKF
                     PNVAAAVTVAAVAMPLNLALAVASGLPPAAGLFAGAIGGCIAAAFGGSPLQVTGPAAA
                     LSVMVLGLSKQFGPVGVAAACVMIGILQLLLAYSGAGRLGKYVPESVLAGFTSGVGIK
                     LLDTQLPELLGFPEVINYQLIDLAMMMHRPQWLHHVSWVAVVSGLLVAFSMVGLSKYK
                     RLPAAALAVLLVTFVSVYVGWKVERIGDIGNVPSRFPALSIPLVEDEQWLDLIVATLP
                     LGLLAALESLLSAQVLDRMKKDLRPHEPSLELFGQGLANLAVGLFQGMPVTGVVVRSG
                     VNVQSGATNRLSSLLHGVLLALLVLSLAPLIRNIPLAALAGLLCVVGFRLIEWRTLLA
                     LAKKEKLEAAAFVMTALGTVSGHLMSGLVLGGLLHVASLLLHRGKASTSLHSGAAKLR
                     GVRATLGAEHAQAREVRHGAPLPSHHRWLRHIRHSAVRAKTAYVHPEGSVIGKVVLGD
                     HVHVAADTSIRADEGSPFFVGANSNLQDGVILHALKEKYVEVAGESWAIYIGEGVSIA
                     HNALIHGPTYIGDRTFVGFKAVVHDSIVGAGCYIGIGAIVIGVEIPEGRFVPHGTIVD
                     SADAVDRLPLASEHHREFNEDVVEVNRGLAVAYAAADDESEARNPVAEIRPEPNANPW
                     QERWLVPANVEKF"
     gene            4997..5935
                     /locus_tag="PKL17_19265"
     CDS             4997..5935
                     /locus_tag="PKL17_19265"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK46933.1"
                     /translation="MFYSGLLVGLLLLAMFVAIRSVVYVVEGEVVVLSRFGRALRDSS
                     GQILWLSAGLHVKQPWDDVLRVSIKEQCIELDSDEDLSVMTNDGIAVRYQCAMRMAPL
                     VDKLEHHLFGLARPTDHVVGTFSCLLRNEIANFSVPAGDGIVGTSGTSNVEQLIDSSV
                     GAYAMIRRERRTLNERVTSCGQRLIGDQFGIRLEAIDVIRFDPPDELRDALNTVMQAK
                     NDVDASLYRAEGDCQQKLLSARKGVAIATERARAIEVEMSELANKLSELAKSHVLPDY
                     VARRRAEVLSEARQVYVRSSLKTPAASAASAQKVNP"
     gene            5932..>6591
                     /locus_tag="PKL17_19270"
     CDS             5932..>6591
                     /locus_tag="PKL17_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNK46934.1"
                     /translation="MTSYWPFVLGILLPCGIVLLAFWALRSFSVSVEDGEVVLCERFG
                     RHVATLREPGLHLIWDKLFPWVSVRRISLRRDFLKLSDVHVADREGTTVIVDVWIDYR
                     VTDPLKAEYAVANLEESLRSLVMHAALSLLGRRTFYEILCDRCELAEQLRKDIRPDTE
                     RWGVGIELVFLQKVSLLPEVATQILSTIAARLGRARAELIEEGPLVVATLEAKTSVDV
                     AA"
BASE COUNT         1190 a         1887 c         2097 g         1417 t
ORIGIN      
        1 cggttgttca ccaggttcgg atcatccacg cccggcgagc cgacctggcc gacgtgcgcc
       61 ggcgtgccgc ccgtcgtccc ggccggcagc atgaccttca ccgtgatcgg cggcgccgtg
      121 ccctgcggca gatccgggcg ggtgcaggtg aaggtgtcgc cctgctgcgt gcaggtccag
      181 cccggacccg gcgtcaccga gtcaatcaca ccacccggcg gcactttgaa ggtcacatgc
      241 agcggaccgg gatcgccgga gttgccgacg ttcttcacat caatcgaata cgtcaccggg
      301 tcgcccggct gcgggaacgg cggattcttg ctcacgatga cgcgcagatc cgcctgggtg
      361 ttcggcaggt tcaccaccgt cggtgccgcc ggctgctcac cgttgcccga ggggaaggtc
      421 gctggcggca gcgcagggtt gcccgcggtg gtcgccgtcg tcgtgctctg cgtcgggatc
      481 ttcgacagcg tcgtgttcgg atcggtcttc agcttgtact gcaccaccac gggcgagtcg
      541 ctcggcgaca gacggccacc ggagtttgca gtcgcgccct tgccaatgcg aatcaccagc
      601 accggcttgc cggtcatcgg atcattgata acttccgctt gatcgtcgcc tggcgtgtcg
      661 ctcttggggc cgttttgacc ggcttcggga ccgctcacaa acacaatcga gcctggcaca
      721 tacgtcatgc catcgggcag cgggtgacgg atcaccacgt cggtgccatc gtcatcgccg
      781 acattcttgc tggtcgatgt aaacgttacg gtgtcgcccg gacgaacaga gcttgggtca
      841 gccatcagcg tcgtttcgat gaccggcttg cggctgcgaa tcgaagtgaa caggccaccg
      901 acgaagtaca catcatcgat cgagttggcc tggatcgtgg cctgggtatc gtttgccttc
      961 agcagcgacg aaacatcaat ctggtcgagg tcgacgcccg acatgctgcc cgccgttccg
     1021 gtaagctgcg gcaagtcacc cgtcaccgac accgccgcgc cgagcgtgct gcgggtgctg
     1081 ttgaagatgt tgtcggcggg gtttattccg tccgtgaccg gcttgttgtt aaacaggaag
     1141 gagtcttttt tgtcgctgtc tccttcgtag gcaatcacgc cgagcttgcc ctgcggaacg
     1201 ccgccttcgg ggacgcggaa tccaatcaca ccgaggttga cgctgcggcc aatgtcaacg
     1261 ctgtcgagtc cgtcatagat cgtgatgttg cggaccggct ctttgtcgtt gcgatagaca
     1321 accaacatgg tccaggcgtt aaactgcacg tcttcgtcgc ggtttacgac gttgcggcga
     1381 acgacgttgc cgatgcggta ggcgccggtg ccgtagcgct gcacaatcgg cgtcacatcg
     1441 tagctgccct gatagacgtg accgccgcca ccaccgactg cggtgacgac atcggtggca
     1501 gggtttggca gaagcatcat cgaaaagccg cccggtccca ctttttcaaa gctgaccgtg
     1561 ctgttcgcga gcgcgatgtc ttcgcccagg tttccgctcc agtacaggcg tgcgtacagc
     1621 acctgcgagc cctgcggtag cgagagcatc gacgtgctgc gcgcatcgcc aatcatcgtc
     1681 tgcccgtccg ctttcgcggt tgagtcatcg gcccgccaca gcacgtccgc cgcgctgtca
     1741 tcaatggtga tggggtcgcc gcagtttccc accgttccaa ccaccggctt gggaatcgac
     1801 atgcccaggc tgtcgacggc tcggcactct tggccaagtg tgttgccgat gagaacgatg
     1861 tcgcctttga cgtcggtctg gaaccgcagc ttcggggctg cgtggacgtt cggcagcata
     1921 gagccgacgg cccaaagcac tgatgccacg atcagttgca gcaggcgcgg gcgatgtggg
     1981 gtgggtcgta ctcgcatcgg atggactcct cctttgggga tcagactgtc catcatatgc
     2041 aagtcacact acatatgcca agcaatccgc gcccaaagcc aggccaagtg ttgaaaaagg
     2101 ctcgtgagtt gtcacccgca aaaagattgc cgctcggttg cgcattttgt atatggatgc
     2161 aaagtgcgta tttggaccac acgctgctgg gttctgctcg cgctgctctc gctcgttttg
     2221 gagctagggc aggcgccgcg gtcacttggt gcgtggtggg gggaatcggc gctgatgccg
     2281 cgcaatccca gtcggaatgc atcgcgatca ctgctgctgg cgaaggcaga atccgcgagc
     2341 gcggacgaaa aggatgacgc tgccgatcct gcgttggatg ccacgcgcgg tgatgacagc
     2401 ggcgcaggct cgggcggcgg caaggaaccg gacaaggacg gacattccga ccgtgattcc
     2461 gatgccactg cggacgatga caccggttcc gaatcagaca gcgatgcaga tgaaggcggt
     2521 gattccgatc ccaagtctgc tgatgaaagc gacgaaaaac cggatggcag ctcgtccgaa
     2581 cagcgcgaag aaacagagcg caaagcgggc gaagaaaacg acgaagaaaa cagcggtcaa
     2641 aaagacgatc cgctgagtct tctgcgtgcc gcagcagttg ctttttcctg tgatctgacg
     2701 ggccaagagc tgcgcttcgg caacacgacg cgcctttgtg atctcacgac ctccgacgcc
     2761 ctcttgcggc ctcctcgctc ggtgtagtcc gaccgaagat ccgcagtgaa ggatggggaa
     2821 gcgcgggctt tccctatgtc gtgccctttt gcaaagggcc tgcgcgcgtg cgcttggttt
     2881 tgcgcatgcg tgttcgcgag ggagtgaaca tgaaacgtct gtacgaccgt ccatatgccg
     2941 gtcgacataa ggattcgccg cctgtgcttg cggcccgaat cggttcgatg tggtcggcgt
     3001 ggctggcaga ctggaaagat gccatctacg agcccaagtt tcccaatgtg gctgcggcag
     3061 tcactgtggc tgcggttgcc atgccgctca atttggcgct cgccgttgca agtggtctgc
     3121 cacccgcggc gggtctgttt gccggcgcga ttggcggctg cattgccgcg gcgtttggcg
     3181 gctcgccgct gcaagtcacg gggcccgcag cggcgctgtc cgtcatggtg cttgggctgt
     3241 cgaaacagtt tggtcccgtc ggagttgccg ccgcctgtgt gatgatagga atccttcagc
     3301 tcttgcttgc gtactcgggc gcggggcggc ttggcaagta cgttccggaa agtgtgctgg
     3361 cgggtttcac aagcggtgtg ggcatcaagc tgctcgatac ccagcttccc gagctgctcg
     3421 gttttcccga agtcatcaac tatcaactta tcgatctggc gatgatgatg caccgtccgc
     3481 agtggctgca tcatgtgtcg tgggtcgcgg tggtgtcggg tctgctcgtt gcgttttcga
     3541 tggtcggtct gtccaaatac aagcgactcc cagccgcggc gcttgcggtc ctgctggtga
     3601 catttgtgtc ggtgtacgtc ggctggaaag tggaacggat tggcgacatc ggaaacgtgc
     3661 catcgcgttt ccctgcgctg tcgattccgc tggtggaaga cgaacagtgg cttgatctga
     3721 tcgtggcgac gttgccgctt ggtcttttgg cggcgctgga atcgcttctg tctgcgcagg
     3781 tgctcgatcg aatgaaaaag gatctgcgcc cacacgagcc aagcctagag ctgtttgggc
     3841 agggacttgc gaacctggcc gtgggactgt ttcaaggcat gccggtgaca ggtgtggtgg
     3901 ttcgttccgg agtcaacgtc cagtccggtg caaccaaccg tctgtcgtcg ctcctgcatg
     3961 gcgtactgct cgctctgctg gtgctttcgc tggctccttt gattcgcaac attccgcttg
     4021 ccgcactggc gggacttctg tgtgtggttg gcttccggtt gattgagtgg cgcacgctgc
     4081 ttgcgctcgc gaaaaaagaa aaactcgagg ccgcagcgtt tgtgatgaca gcgctaggaa
     4141 ccgtgagcgg acacctgatg agcgggcttg ttcttggcgg gctgcttcat gtcgccagtc
     4201 tgttactgca ccgcggcaaa gcatctacgt cgctccacag tggtgctgcc aagctgcgtg
     4261 gggtgcgggc cacgctcggt gctgagcatg cacaggccag agaagttcgg cacggtgcgc
     4321 ccctgccttc ccatcatcgc tggctgcgtc acatccgtca cagtgccgtg cgcgcaaaga
     4381 ccgcttacgt tcatccggaa ggaagcgtga ttggcaaagt ggtgctgggg gatcacgttc
     4441 acgttgccgc agatacgtcg attcgtgcgg atgaggggtc gccgtttttc gtcggtgcaa
     4501 actccaactt acaagacgga gtgattctgc acgcgctcaa agaaaagtat gtcgaagtcg
     4561 ccggggaatc ctgggcgatt tacatcggcg aaggcgtgtc gattgcgcac aacgccctga
     4621 tccatggtcc aacgtacatc ggtgatcgca cctttgtcgg cttcaaagcg gtggtgcacg
     4681 actcgattgt gggggccggc tgttacatcg gaattggcgc gatcgtcatt ggcgtcgaaa
     4741 ttccggaagg tcgctttgtg ccgcacggaa cgattgtcga tagcgccgat gccgtcgacc
     4801 gtttgccgct ggcttccgag catcaccgcg aatttaacga agacgtggtc gaagtaaacc
     4861 gcggtctggc cgtggcttat gcagccgctg acgatgagtc cgaagcgcgg aatccggtgg
     4921 ccgaaatacg acccgaacca aacgcaaacc cttggcaaga acgctggctg gttccagcga
     4981 acgtggagaa attctgatgt tctattctgg actccttgtg ggcctgctcc tgctggcgat
     5041 gttcgtcgcc atacgcagtg tggtctacgt cgtggaaggc gaagtcgtag tcctgagccg
     5101 tttcggtcgt gcgctgcggg attcttccgg acagattctg tggctgtccg cgggacttca
     5161 tgtcaagcag ccgtgggatg acgtgctgcg cgtgtcgatc aaagagcagt gcatcgagct
     5221 ggattccgat gaagacctct cggtgatgac caatgatggc atcgcggtgc gctatcagtg
     5281 cgcgatgcgg atggctccgc tcgtcgacaa gctcgaacat cacctgtttg gactcgcccg
     5341 tccgacagac catgtggtgg gaacgttttc ctgtcttctg cgcaacgaga tcgccaactt
     5401 ttccgtgccc gcaggggatg gaatcgttgg cacaagtggg acttccaacg tcgagcagtt
     5461 gatcgattcc tctgtggggg cctacgcgat gattcggcgt gaacggcgga cgctcaatga
     5521 gcgcgtcacg tcatgcggac agcgtcttat cggcgatcag ttcggaatcc ggttggaagc
     5581 gattgacgtg atccgctttg acccacccga cgagctgcgt gatgcgctaa acaccgtgat
     5641 gcaagccaaa aacgatgtcg atgcatcgct gtatcgcgcc gaaggagact gccaacagaa
     5701 gctcctttcc gcgcgcaaag gagtcgccat tgccaccgag cgtgcgcgcg caattgaggt
     5761 cgaaatgagc gagcttgcca acaagctgag cgagctggca aagtcgcacg ttttgcccga
     5821 ttacgtggcg cggcgtcggg ccgaggtgct gtcggaagcg cggcaggtgt atgtccgttc
     5881 cagtctgaaa accccggctg cttccgctgc atccgcacaa aaggtgaatc catgaccagc
     5941 tactggccgt ttgttttggg aatcctgctg ccttgcggaa tcgtcctgct cgcgttctgg
     6001 gcgctgcgca gcttttccgt atctgtagaa gatggcgaag tcgtcttgtg cgaacgcttc
     6061 ggtcgacacg ttgccacact gcgcgagccc ggacttcatc tgatctggga caagctgttt
     6121 ccttgggtgt ctgtccgtcg catatcgctg cgccgtgact tcctgaagct gtccgatgtg
     6181 catgtggcgg atcgcgaagg aaccacggtc attgtcgatg tctggatcga ctaccgcgtg
     6241 acggatccac tcaaggcaga gtacgcagtg gccaacctgg aagaatcgct gcgcagtctg
     6301 gtgatgcacg cggcactgtc gctattgggg cggcggacgt tttacgaaat cctctgtgat
     6361 cgctgcgagc tggccgagca gctgcgcaaa gacattcgtc ccgataccga gcgctgggga
     6421 gtcggcatcg agcttgtgtt tctgcaaaaa gtgagtctgc ttccggaagt cgccacgcag
     6481 attttgtcga cgatcgctgc acggctgggc cgggcgcggg cggaacttat cgaagaaggc
     6541 ccactggtgg tcgcgacgct ggaagccaag acaagcgtgg atgttgcggc g
//
LOCUS       DAORGN010000247         6574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5529_length_6574_cov_6.226108, whole genome shotgun sequence.
ACCESSION   DAORGN010000247 DAORGN010000000
VERSION     DAORGN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_6574_cov_6.226108"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKL17_19275"
     CDS             complement(<1..337)
                     /locus_tag="PKL17_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNK46935.1"
                     /translation="MEKQKRAFGYVRVSTDMQAQFGLSLEAQQAQIAGYAQVYGYTMA
                     GFYVDHDSAKNTTGRPEYQKMMAEVDKGKAEFIISTALDRFSRSQRDFLAFQDDYVKT
                     DRVHLVLINQ"
     gene            complement(378..1043)
                     /locus_tag="PKL17_19280"
     CDS             complement(378..1043)
                     /locus_tag="PKL17_19280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK46936.1"
                     /translation="MRKRVGCATTLHAPTPTARTCPHLSSLRRTDRDTPQKSRSAWYD
                     AKSGGSGNVLPEQSTMIGQLVGHYKVVRKIGDGGMGSVFEAVHETIGRRVAIKVLKPQ
                     YSKDKNVVTRFFNEARAVNIVSHPGVVGSFDYGEMPDGTAYIVMEYLEGESLSSRIKR
                     TAGPMGFDALRIGRQIGAALAAAHSKGIIHREVLMIDSNCLRISGKPLHRRRQDYGKL
                     QTV"
     gene            1087..1689
                     /locus_tag="PKL17_19285"
     CDS             1087..1689
                     /locus_tag="PKL17_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNK46937.1"
                     /translation="MKDFEKDALDEACVLAQNRAFYAALRDRNVELMGQVWSQSAQVS
                     CIHPGWQPLHGRETVMASWETILQHSNLPAIYPQAASVHLWGDTALVICEESFREGRL
                     VATNIFVREADDWRLLHHQAGPLNPSADIDDVDSLFDDGDPDALLGLHDDDPAADAGE
                     HAALSADSLDDVDNPSSAQSSADELLALYAPRRRTQRFVN"
     gene            complement(1712..2719)
                     /locus_tag="PKL17_19290"
     CDS             complement(1712..2719)
                     /locus_tag="PKL17_19290"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNK46938.1"
                     /translation="MSSAPQPDQPKLSTQARQRLAAAGGLFVLICIGAWFYVDSLDSP
                     RGQTKQPVSALRQRYPDLLPRRLPDSMAVWRRSTEAAESSYGPPRPPRMVRPDLSADA
                     RRQKIRAMLQKQNLDLQSINPAVPGFSTPNFSPSYPLRPEEVQVKLGATVLELVASRD
                     EGDVWFYGVLKNTGEGPIDHPRIDVTMWNEGRTEKVGSAYGFTDRFVLGPNEVTPFRV
                     LAKAAPRFANATATVEVRRPFTDRRLGKLQLVQHTLTQQSPLILIKGTVKNIDDKPQH
                     FVKVIALARDTKGRPIGYASAYVTTKRLAPGASASFSSYFLLDRKPVHIDFDLEGSPA
                     D"
     gene            complement(2780..3499)
                     /locus_tag="PKL17_19295"
     CDS             complement(2780..3499)
                     /locus_tag="PKL17_19295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNK46939.1"
                     /translation="MKTSHLALTLSLSLAGFSGLALAAPPDPTGGKFTIEEATKGLGK
                     GTLTAKIETTMGAFHCELFEQDAPGTVANFVGLARGLRPFVDPGTGDWVRRPFYDGLI
                     FHRVIPTFMIQGGDIKGNGTGEPGYTIADEKNAPHSFSRGGILAMANRGRNTGGSQFF
                     ITEQEQPPLDDGGAAGGHYQIFGQCPEVDLVKKIARVPRNPMDRPLSDVKITKVTIER
                     VSKEPAQTPKTAAKPAAKPSK"
     gene            complement(3554..4177)
                     /locus_tag="PKL17_19300"
     CDS             complement(3554..4177)
                     /locus_tag="PKL17_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46940.1"
                     /translation="MLWSVFDELVSAMASDAEKLLAARRTYSQKTGTVEPDHELYAER
                     SDAFVEWFLFELRDEEGLTSVERRICELPSEDVHMPHLLALRASYRSLFQVVRQQPGG
                     LLLDDLVGGGRFSVDERRRLPGVSAGDLFEARLIPDPDRRPALCFGRALQFHPREAAD
                     SVLAQIRLAKQNAEPKAELLSRLARLRLRCFSYRHVPAARIYAPFAG"
     gene            complement(4182..5150)
                     /locus_tag="PKL17_19305"
     CDS             complement(4182..5150)
                     /locus_tag="PKL17_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46941.1"
                     /translation="MSWRAATLFFGLCGLIGLVSLGGCNDPGPTLPDFSSDMSSGVSD
                     MRQTCDGSVNIPVDMKGGGSGRTIKPGDIGSPCSKDSDCQQGSGQRVCFKTNLLNDPK
                     GAATPSGYCSAVCGGDADCGSGNYCLSFPPSVSAYCVAGCSDAATCRKPDYLCMFLDT
                     VSACLPRFASMTCNPGENKACKTVLTSPDNLDGGCVRQAYEDDRSGICYPTCKLGASC
                     PQDTSGSTLTCRFLSSYPTGDSYAGNLCLPTPSVSLKPEGSTCLFADECDTGMQCDLP
                     YVNDGQASRKCRKLCDLSAATSTCPSGQACRNVFGTCSGAGLCRPQ"
     gene            5387..>6574
                     /locus_tag="PKL17_19310"
     CDS             5387..>6574
                     /locus_tag="PKL17_19310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNK46942.1"
                     /translation="MENAVSAKTQRRAIDEATHLTPAGIPLPVEPPGFLGHPRAFWTL
                     AFVELWERFGFFLMMSLLVLYMNERWHLSEAEAMRWFSYYITAVYFSPLLGGYIAERG
                     LSRPHWVAIGASLLSIGYFGFALAPRETMVVWFGLLVIGNGLFKPNIATLVGSLYAPG
                     DTRRDGAFSYFYFAVNVGGAISPVVGGYLRMHFGWRVAFSAAGAGLILAVLTLLSFSR
                     RYKQQMQQGATAAAEVEHATSVRSRVGALFLISACALPFWTAIMQSGTSLALWARDNT
                     DRTLSLFGRPTEFAPEQFSSLGSWFILLLTVPLTAVLDRVNRGRGVHSAVKIVVGLLM
                     AATAFGLMSMVSASSHGRTSMLWLVSYYFVFTIGELLLSPVGLSIVSKLAPPRWVGVM
                     IGVW"
BASE COUNT         1202 a         1874 c         2089 g         1409 t
ORIGIN      
        1 cctggttgat tagcacgaga tgaacgcgat cggtcttcac gtaatcatct tgaaaagcca
       61 gaaagtcacg ctgcgaacga ctaaaccgat ccagcgctgt cgagatgatg aactcagctt
      121 ttcccttatc cacctcggcc atcatcttct gatactcagg ccgcccggtc gtgttcttcg
      181 cagagtcgtg atcaacatag aagccagcca tcgtatagcc atatacctga gcatacccag
      241 cgatctgtgc ttgctgtgcc tcaagtgata ggccgaactg tgcttgcatg tcggtagaca
      301 ctcgaacgta tccaaacgcc cgcttctgct tttccatgac gcccccgtct gcgctcctct
      361 gtgtttgagt cctactttta gactgtctgt agtttgccat agtcctgtct ccgtctgtgt
      421 aacggctttc cgctgatgcg gagacaattg ctatcaatca tcagaacttc tctgtgaatg
      481 attcctttgc tgtgggcggc ggcgagggcg gcaccgatct ggcgaccgat gcggagcgcg
      541 tcgaagccca tcggtcccgc ggtccgcttg atccgtgacg agagcgattc gccctcgagg
      601 tattccatga cgatgtacgc cgtaccatcg ggcatctcgc cgtagtcgaa actaccgacg
      661 acgccgggat gggacacgat gttcaccgcg cgggcttcgt taaagaagcg ggtgacgacg
      721 tttttgtcct tggagtactg gggtttaagc accttgatcg cgacgcggcg accgatcgtc
      781 tcgtgcaccg cttcgaagac gctgcccatg cctccgtcgc cgatctttcg gacgactttg
      841 tagtgtccaa cgagttgacc gatcatggtg ctttgctcgg gaagaacatt cccactaccc
      901 ccacttttgg catcgtacca cgcggagcgg cttttttgcg gagtgtcccg atcggtacgg
      961 cgcaggctcg acaaatgcgg gcaggtacgc gcggttggcg tgggcgcgtg gagtgttgtc
     1021 gcgcagccga cccgcttccg cattgcaaag gagtacggtt tgtcgtatcc gcagtgcgtg
     1081 acgcagatga aggactttga gaaagacgcg ctggacgagg cgtgtgtgct cgcccaaaac
     1141 cgtgctttct acgcggccct gcgcgaccgc aacgtcgagc tgatggggca ggtgtggtca
     1201 cagtccgcgc aggtttcgtg cattcatcca ggctggcagc cgctgcatgg acgtgagacg
     1261 gtgatggcca gctgggagac gatcctccag cactcgaact tgccggcgat ctatccgcag
     1321 gcggcgagcg tccatttgtg gggcgatact gcgcttgtga tctgcgaaga gtcgtttcgt
     1381 gagggccggc tcgtcgcgac caacattttt gtgcgcgagg ctgatgactg gcggctcctc
     1441 catcatcagg ccgggccgct caatccttcg gcggacatcg acgatgtcga ttcgctgttc
     1501 gatgatggcg atcccgatgc gctgctcggg cttcatgacg acgaccctgc ggcggatgcc
     1561 ggcgaacatg cggcgctgtc cgcggactcg ctggatgacg ttgacaaccc atcctcggcg
     1621 cagtcgtctg ccgacgagct cttggcgcta tatgccccac ggcggcgcac ccagcgtttt
     1681 gtaaactgat gggccgatcg ggcctatggt gtcagtccgc cggcgagcct tcgaggtcaa
     1741 agtcgatgtg gacgggcttc cgatcgagca gaaagtagct cgaaaacgac gcactcgcac
     1801 ccggcgcgag ccgtttcgtc gtgacatacg ccgaggcgta gccaatcgga cgaccctttg
     1861 tgtcccgcgc gagcgcgatc actttgacaa aatgctgcgg cttgtcgtcg atgtttttga
     1921 ctgtgccctt gatcaaaatc agcggtgact gctgcgtcag ggtgtgctgg acaagctgca
     1981 atttgccaag cctgcggtcg gtgaatggtc gtcgaacctc gacggtggct gtggcgttcg
     2041 caaaacgcgg cgcggcttta gccagcaccc ggaatggcgt cacttcattt ggacccagta
     2101 caaagcgatc ggtgaagcca tacgcagatc cgactttttc tgtgcggcct tcgttccaca
     2161 tcgtgacgtc gatgcgtggg tgatcaatag gcccttcgcc cgtgtttttc agcacgccat
     2221 aaaaccaaac gtcgccctcg tcgcgggatg cgaccagctc gagcacggtc gctccgagct
     2281 tgacctgcac ttcttccggg cgcagcggat aggacggtga aaaattgggc gttgaaaagc
     2341 cgggcaccgc gggatttatc gactgaagat cgagattttg cttttgcagc atcgcgcgga
     2401 ttttttgccg acgggcatct gccgacaaat ccggacgaac catccgcggc ggtcgaggtg
     2461 ggccgtagct gctctcggca gcctcggtgc tgcgtcgcca caccgccatc gaatcgggaa
     2521 gccgccgcgg caaaaggtcg ggatagcgct gtcgcagcgc cgacacaggc tgcttggttt
     2581 ggccgcgcgg cgagtccagc gaatcgacat aaaaccaggc gccgatgcag atcaggacga
     2641 acagtccacc agccgccgca agccgctgcc tggcttgtgt tgacagcttt ggctggtccg
     2701 gctgcggcgc gctgctcatc ccagcgttta gactaccctg ccaggccggc caacgacaaa
     2761 agcagggttt ctagacggct tacttagacg gcttggcggc gggcttggct gcggttttgg
     2821 gcgtctgcgc cggctctttg gatacgcgct cgatcgtgac tttggtgatt tttacgtcgg
     2881 acagcggacg atccatcgga tttcgcggca cgcgggcgat tttctttacc agatcgacct
     2941 cggggcactg accaaagatt tgatagtgac cgccggcagc gccgccatca tcgagcgggg
     3001 gttgctcttg ttcggtgatg aaaaactgcg atccgccggt gttgcggcct cggttggcca
     3061 tggccaggat gccgccgcga gagaacgagt gcggggcgtt tttttcatcg gcgatggtgt
     3121 aaccgggttc gccggtgccg ttgcccttga tgtcgccgcc ctgaatcatg aacgtcggga
     3181 tgacgcggtg gaaaatcagg ccgtcgtaaa acggacgcct tacccagtcg ccggtgccgg
     3241 gatcgacaaa cggacgcagg ccgcgtgcca gaccgacgaa gtttgcgacg gtgccgggcg
     3301 catcttgctc gaacagctcg cagtgaaatg cgcccatcgt ggtttcgatt ttcgcggtca
     3361 gcgtgccttt gccaagtccc ttggtcgctt cttcgatggt gaacttgccg ccggtggggt
     3421 caggcggagc ggcgagtgcc agaccagaaa aaccagcaag tgagagcgac aaggtgagcg
     3481 caaggtgtga cgtcttcatg gcaagttctc ctctttcgga atgtgggtta gacgcttgga
     3541 gttcgcgccg tcgtcaaccg gcaaaggggg cgtaaatgcg cgccgccggg acgtgacggt
     3601 acgaaaagca gcggagtcgc agtcgagcca gtcgggacaa aagctcggct ttcggctcgg
     3661 cgttttgctt ggcgagtcgg atttgtgcga gcaccgaatc ggcggcttcg cgtggatgaa
     3721 actgaagcgc tcggccaaag cacaaagcgg gacggcgatc gggatcggga atcagccgcg
     3781 cttcgaagag atcgccggcg ctgacacctg gcagtcgtcg ccgttcatcg acggaaaacc
     3841 gcccacctcc gaccaggtcg tcgagcaaaa gaccgcccgg ctgctggcgc accacctgaa
     3901 acaagcttcg ataggacgcg cgcagcgcca aaaggtgtgg catgtgcacg tcttcgctgg
     3961 gcagctcgca gatgcggcgc tcgacgctgg tcaggccttc ttcatcgcgc agctcgaaca
     4021 aaaaccactc gacgaaagca tctgatcgtt cggcgtatag ttcgtgatcc ggctcgacgg
     4081 tgccggtctt ttgcgaatat gtccgacggg ccgcgagcag tttttcggca tcgcttgcca
     4141 tcgcagaaac cagctcgtcg aaaaccgacc acaacatgcg attactgcgg acggcagagc
     4201 ccggcgcccg agcaagtacc aaagacgttt cggcaggctt ggcccgacgg acaagttgat
     4261 gtggccgccg atagatcgca gagctttcgg cacttgcggc ttgcttggcc gtcgtttacg
     4321 taaggcagat cgcactgcat tccggtgtca cactcgtcgg caaacaggca ggtcgagcct
     4381 tccggtttga gcgatacgct gggggtcggc aggcagagat tgccggcata ggaatcgccc
     4441 gtcgggtaac tgcttagaaa acgacaggtc agcgtcgatc cactcgtgtc ctgcggacag
     4501 cttgcaccga gcttgcacgt cggatagcag atgcccgagc ggtcgtcttc gtaagcctgg
     4561 cgcacgcagc caccatcgag gttgtcgggg ctggtcagga cggtcttgca ggctttgttt
     4621 tcgccggggt tacacgtcat gctggcgaag cgcggcaggc aggcgctcac cgtgtcgaga
     4681 aacatgcaca agtagtcggg tttgcggcag gttgcggcgt cgctacaacc ggcgacgcaa
     4741 taggcgctca ccgacggggg gaagctaagg cagtagttgc cgctgccgca gtccgcatcg
     4801 ccgccgcaca ccgccgagca atagccgctg ggagtggctg cgcctttcgg atcgttgagc
     4861 aggttggtct taaaacagac gcgctggccg ctgccttgct gacagtcgct gtctttgctg
     4921 catgggctgc cgatgtcacc gggcttgatc gtgcgaccgc tgccgccacc tttcatgtcg
     4981 acggggatgt tgacggaccc atcgcaggtc tggcgcatat cggaaacgcc gctcgacatg
     5041 tcgctgctga aatctggcag cgtcggtcct ggatcgttgc aacccccaag gctgacaagc
     5101 ccaatgagtc cgcaaagacc gaaaaacagc gtcgccgcac gccagctcat gtttcgagac
     5161 ggggggcgaa gcgaggatga acgcagcatc agcgaagact ccaaggttgg gaagcgggat
     5221 tagcagcgat tcggcggcgg gtcaagaaag caagcggttc ggcccgacgg taagaaaaaa
     5281 atcgcgaggg aaactttgcc ggcagccagc ttggggcgtt attgtgcgcg cggctgccca
     5341 atgtgcagcg atttctcgtc gctgctagca ggctggagta tgacgtatgg aaaacgcagt
     5401 gtccgccaaa acccaaagac gagccatcga cgaggcgacg catcttacgc cggctggcat
     5461 acctcttccc gttgagccgc ccggttttct gggccatccg cgggcttttt ggacgctggc
     5521 tttcgtcgag ctgtgggaac gctttggctt tttcctgatg atgtcgctgc tggtgcttta
     5581 catgaacgag cgctggcacc tgtccgaagc cgaggcgatg cgatggttta gctactacat
     5641 caccgcggtg tatttttcgc cgctgctcgg tggctacatc gccgagcggg gcttaagtcg
     5701 tccacactgg gtggcgattg gcgcgtcgct tttgtcgatc ggctatttcg gttttgcgct
     5761 cgctccgcgc gaaacgatgg tggtgtggtt tggcctgctg gtgatcggaa acggcctgtt
     5821 taagcccaac atcgcgacgc tggttggcag cctgtacgca ccgggagata cgcgaagaga
     5881 cggtgccttt tcgtacttct atttcgcggt caacgtcggt ggagcgatct cgccggtcgt
     5941 cggtggttat ctgcgaatgc actttggctg gcgcgttgcg ttttcggcag cgggggccgg
     6001 gctgattctg gcggtgctga cgcttttgtc gttttcgcgt cgctacaaac agcagatgca
     6061 gcagggagcg acggcagcgg cagaggtgga acatgcaacg tcggtccgaa gtcgcgtcgg
     6121 tgcgctgttt ctgataagcg cctgcgccct gccattttgg accgcgatca tgcagtcagg
     6181 tacgtcgctg gcgctgtggg cgcgtgacaa caccgatcgc acgctgtcgc tgtttggccg
     6241 tccgaccgag tttgcccccg agcagttttc gagcctggga agctggttca ttttgcttct
     6301 gacggtgccg ctgacggcgg tgctcgatcg ggtcaatcgc gggcgcggcg ttcacagcgc
     6361 ggtcaaaatc gtcgtcggtt tgttgatggc agcgacggca tttggtctga tgagcatggt
     6421 ttcggcgagc agccacgggc ggacgtcgat gctgtggctc gtcagttatt actttgtctt
     6481 taccatcggc gagctcttgc tgtcccccgt cgggctttcg attgtcagca agctggcgcc
     6541 gccgcgctgg gtcggcgtga tgattggtgt gtgg
//
LOCUS       DAORGN010000248         6447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5695_length_6447_cov_7.037078, whole genome shotgun sequence.
ACCESSION   DAORGN010000248 DAORGN010000000
VERSION     DAORGN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_6447_cov_7.037078"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PKL17_19315"
     CDS             complement(<1..1361)
                     /locus_tag="PKL17_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK46943.1"
                     /translation="MLSYLRISNFAIIEEVELTLGPGLTVLSGETGAGKSLILDAVAL
                     LRGGRATADVIRSGADEARIEALFSPAPESPATQALIDRLHRLGIDPKGIAEDGLVVR
                     RVIGRGGRNRIHIQGQLSTATALAEVCGGLIDVSGQHEHQSLLDVGSHLGLLDRFGVP
                     VQLQTDMEQAFLALHAASEELQAASLDEKSRSEREEFLRFQLQELDQADPQPGEDESL
                     RNEQRRLRSVEKLAQAGRRSESRLYSGEDSVLDGLSAVLRELREVAGLDAELQELTQR
                     IAEAQLLLGDAAHDLRRYVDGLAADPERLATVEDRLHLLSRLLRKHGPDLPSLLQKRD
                     DMQRELLRLTSHEARRLDANQAVEIAQKAACQTAKKLSDARQKAAQALATKVEKELAD
                     LAMEGARLVPQVLPRPASRGDDPALVVSVDAGDLRRLGRDGWDRCELLFSANHGEAPR
                     PLQR"
     gene            1512..1961
                     /locus_tag="PKL17_19320"
     CDS             1512..1961
                     /locus_tag="PKL17_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46944.1"
                     /translation="MHTLVAERFATYVRKQWAPAGFLVSEVSEAPYKELVAVKDGWSF
                     GLSVARESNPDCKARIGIFFRPAPRGSISDLAALRTHYQTKLAQQRRGSRIKNCGLPI
                     GQVFLFFETTGQLGESAVAEWVSMVDELSDRAFDPDGLDELLGVAQE"
     gene            1968..2849
                     /locus_tag="PKL17_19325"
     CDS             1968..2849
                     /locus_tag="PKL17_19325"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNK46945.1"
                     /translation="MRTSRTFAKRLASILTVFGMSATGCIETPPSITAVDSGVDVMID
                     MSVPFVTAQVRFGNFVAGAAPVDLCLKAPSDSTWTGPVIRNMAMRPGGVSYLNVSQYV
                     TLNAGTYTVRAVPGSRSDCSVAYGGLPDLALPQIAAGRTYTLIPFGDQTRIGTIKINL
                     FEDDLGSQGGQVRLRFINVSPDVPTADFGFGIGAGYKPLLTDALRGDLGRSAGMTYAT
                     IVPQTNGSASVRPSGMTTDLLTTGAVVNFAAGAVFTTLIGGLPSKASTDPLALKLIAC
                     EDSKAPVSGLSICTQLN"
     gene            complement(2881..3225)
                     /locus_tag="PKL17_19330"
     CDS             complement(2881..3225)
                     /locus_tag="PKL17_19330"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNK46946.1"
                     /translation="MKFRKLLLPLALATLTLSGMPSVRAENAARPQVETANKSTGVVN
                     INEATPEQLERLPGIGPSRAQAIVNFRKTHPFKRIEDLQRIKGLGKKTWNRLRPLLAL
                     SGPTTLASASGQ"
     gene            complement(3304..3624)
                     /locus_tag="PKL17_19335"
     CDS             complement(3304..3624)
                     /locus_tag="PKL17_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46947.1"
                     /translation="MNNPAYATAGTDGMAQRGKSLSEAGPSTAERSAWKAVYCIAERG
                     KGRRLWLRIGTAFPNRDGSLTIRLDAMPLSGQLQVRDPFIGGSRESESEPLPLERPLK
                     RDSA"
     gene            3884..4468
                     /locus_tag="PKL17_19340"
     CDS             3884..4468
                     /locus_tag="PKL17_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46948.1"
                     /translation="MRRPFGRYSEQVPPVFLQSWGPLLVVIHALSAVVLCGASVHQAV
                     LATLLLLRRAVRGRLLRVYSVTTLLSYLATVTGGALLYPRYRVVIRALFLDQNAPWAA
                     NLFDFKENLATLGLPLAVGALLLAMPLSQRSARTLPTPGDVVTDADPAADSDAAADSQ
                     RLAYLTFLVMALGTALVAVINLIAGLVCTAVRGA"
     gene            4519..4938
                     /locus_tag="PKL17_19345"
     CDS             4519..4938
                     /locus_tag="PKL17_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46949.1"
                     /translation="MTSAVFFVTGVVALPVPLYVPLVHRWVMSASAAEQLGPLGSLTM
                     DYFGRSLLAVGCGAVTTLLGMICLRLRHRTSSLVDGKSSPGTLRKPSSIQLQLALAYA
                     VTAFLLCAALFAYKLYGREPSSPVLPDPSPISADRLP"
     gene            5222..>6447
                     /locus_tag="PKL17_19350"
     CDS             5222..>6447
                     /locus_tag="PKL17_19350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNK46950.1"
                     /translation="MLSLASAEASPRKAGLPSKLSLFSRVLSYVESQYVEDVDQDELV
                     YGAIKGMLDSLDPHSTFLRPEQYRELKNDTSGQFAGIGIEVDQRDGLLTVVSVIDGTP
                     AARAGLLAGDQILRIDDQPTRGMMDYIQKMRGKRGIQVTLTVLRSGWKDPRNFAMLRD
                     IIKIRSVEGFLLEPGYGVLKVKQFTDNIDREVDAQLQAMEKESGGKLSGLVLDLRNNP
                     GGLLDQAVRLADIFIESGLIVRTEGRSGRILDEERARPEGTRLGFPIICLVNGGSASA
                     AEIVAGALQDHGRALVMGTQTFGKGSVQTIVELEDGSALKLTIARYYTPSGRSIQEKG
                     ITPDVVVDQVRLADGKLVVRDEPSQKERDLQGHLKNRQKQTVESGPPPGYSVIAQHAP
                     QGEDFQLRTAYDYLKA"
BASE COUNT         1154 a         1873 c         2042 g         1378 t
ORIGIN      
        1 cgctgcagtg gtcggggagc ttcaccgtgg ttggcagaaa acagaagctc gcagcgatcc
       61 caaccgtccc gacccagccg tcgcaggtcg cctgcatcca cactgaccac caacgcggga
      121 tcgtcgcctc ggctcgctgg acgcggaagc acctgcggaa ccagccgcgc tccctccatc
      181 gccaaatcgg ccagctcttt ttcgaccttt gtcgccagtg cttgggccgc tttttgcctc
      241 gcatcactga gttttttggc ggtttggcag gccgcttttt gcgcgatctc gaccgcttgg
      301 ttggcatcca gtcgccgcgc ttcgtgggaa gtcaggcgca acagctcgcg ctgcatgtcg
      361 tcgcgttttt gtaaaagcga gggcagatcc ggaccgtgtt ttcgcaaaag ccgcgacaaa
      421 agatgcagcc ggtcctcgac ggtcgccagc ctttcgggat cagcggccag cccatcgaca
      481 tagcgccgca gatcgtgggc cgcatcacca agcaaaagct gcgcttcggc gatccgctgg
      541 gtcagttcct gtagttcggc atcgagccca gccacttcgc gcagctcgcg cagcaccgcc
      601 gaaagaccgt caagcacgct gtcttccccg ctatacagtc gagattcact gcgacggcca
      661 gcctgtgcca gtttttcgac cgagcgaagc cgccgctgct cgttgcggag cgattcgtct
      721 tcaccgggct gcggatcggc ctgatccagt tcctgaagct gaaagcgcag aaactcctcg
      781 cgttcgctcc ggcttttttc atcaagggat gcggcttgta gttcctcact tgcggcgtgc
      841 agcgccaaaa aagcctgctc catgtcggtc tgtagttgca cggggacacc gaagcgatcg
      901 agcagtccca agtggctacc gacgtcgagc aagctctggt gttcgtgctg cccgctgaca
      961 tcgatcagac ccccgcagac ttccgccaga gcggttgccg tcgagagctg accttgaatg
     1021 tgaatccggt tgcgtccgcc acgccctatc acccgacgga ccaccagtcc atcttcggcg
     1081 attcctttgg gatcaatgcc caagcgatgc agtcgatcga tcagcgcttg ggttgcggga
     1141 ctttcgggag ccggggaaaa cagtgcttcg attcgcgctt catcggcacc gctgcggatc
     1201 acatcggcgg tggctcgtcc gccacggagc agcgcgaccg catcgaggat gagcgacttg
     1261 cctgcgccgg tctcgccgct gagcaccgtt agccctggcc cgagggtgag ttcgacttct
     1321 tcgatgatcg caaagttgct gattcgcaag tacgacagca tggggcggca tcttagcaac
     1381 cgggcctgac gttcccggtg gactgtgttt tgtgtcgtcg gctggcacag ggctgcacgc
     1441 tgcttatccc tgcgaaaaat cgaaattggc tacgataaga ggcgatctgt cagcttgaag
     1501 ggatgtcatc catgcacacg ctcgttgcag aacgatttgc cacctatgtt cggaaacagt
     1561 gggcacccgc cgggtttctg gtaagcgaag tcagcgaggc tccatacaaa gagctggttg
     1621 cggtcaaaga cggctggtcg tttgggctat cggtggcgcg cgagtcaaat cccgactgca
     1681 aggcgcggat tggcatcttt ttccgtccgg caccgcgcgg cagcatcagc gatctggcgg
     1741 ctctgcggac gcactaccag accaagctgg cgcagcagcg tcgcgggtca cgaatcaaaa
     1801 actgcgggct gccgattggg caggtgtttc tgttttttga aaccacaggc cagctcggcg
     1861 aatctgccgt cgctgaatgg gtctcgatgg tcgatgagct gtcggatcgc gcgttcgatc
     1921 ctgatgggct tgatgaactc ctcggcgttg cgcaagagtg aatcaacatg cggacaagca
     1981 gaaccttcgc caaacgcctt gcttcgatcc tgacggtctt tgggatgagc gccacaggct
     2041 gcattgaaac gccgccttcg attactgccg tcgacagtgg cgtcgacgtg atgatcgaca
     2101 tgagcgtgcc gtttgtgacc gcacaggtcc gtttcggcaa ctttgttgcg ggggccgcgc
     2161 cggtcgatct ctgtctcaaa gcgccgtcgg atagcacatg gaccggacct gtcattcgca
     2221 acatggcgat gcggccaggt ggcgtgtcct atctaaacgt cagccagtac gtgacgctaa
     2281 atgctgggac gtataccgtt cgcgcggttc ccggcagtcg ttccgactgt tcggttgcat
     2341 acggtgggct gcccgatttg gcgctgccgc agattgccgc cggacggacg tacacgctga
     2401 ttccgtttgg cgatcagacc cgcattggca caatcaagat caacctcttt gaggacgacc
     2461 tcggctcgca gggcgggcaa gttcgactgc ggtttatcaa tgtgtcgcca gatgtgccga
     2521 cggcggactt tggcttcggg attggggcgg gctataaacc gctgctgacc gatgcgctgc
     2581 ggggcgactt ggggcgctcg gcgggaatga cctatgcgac gattgtcccg cagacaaacg
     2641 gttcggcatc ggtgcgaccg tcgggaatga cgaccgattt actcacgacg ggcgcggtcg
     2701 taaattttgc cgctggggct gtgtttacaa cgctgattgg tggtttgccg agcaaggcgt
     2761 cgacggaccc tctggccctg aaactgatcg cgtgcgaaga cagcaaagcg ccggtgagtg
     2821 gtctgtccat ctgtacccag ctcaactagc cagcgcacca aaatcccccc caagtcgcaa
     2881 ttactgcccg cttgcagagg ccagcgtcgt tggtccgctc agtgccagca gcggacgcag
     2941 tcgattccag gtctttttgc ccaggccctt gatgcgctga aggtcttcga tccgcttgaa
     3001 tggatgggtt ttgcggaagt ttacgatcgc ctgggcgcgg ctcggcccaa tccccggcag
     3061 tcgttcaagc tgttcgggcg tggcctcgtt gatgttgacg acgccggtgc tcttgttggc
     3121 ggtttcgacc tgcgggcgcg cggcattttc ggcgcgcacc gagggcatac ccgacagcgt
     3181 aagcgtcgcc aaagccaatg gcagaagcag tttgcggaac ttcatcgtga tgatctcctg
     3241 tgtgtttggg gttaatggcg ccgcgcggct ttccgaaacc ctgaaaagcc gcgcggctag
     3301 gtcctatgca gaatcccgtt tgagcggcct ttcgagaggg agtggctcgc tttcgctttc
     3361 ccgagatcca ccaatgaacg gatcgcgaac ctgaagctgg cccgagagcg gcatggcatc
     3421 gagacgaatc gtcagcgaac catcgcggtt tggaaaagcg gtgccgatac gaagccacag
     3481 tcggcgccct ttccctcgct cagcgatgca gtagacggcc ttccatgctg agcgctcagc
     3541 agtggatggt ccggcttcag acagactctt ccctcgttgt gccattccgt cggttccagc
     3601 ggttgcgtag gcggggttgt tcatcggttt gtctccagat tgcgttggca agcttcccaa
     3661 tcgagcagcc gcagcgaccc atgagcacgc cctgtgccag cgccgcaaaa tcgcaacgtt
     3721 tcgtcgctga gacagcctac gcagagcgtt tttgcgcgcc cagttgttcg gaccttgtcc
     3781 ggccagccgg acacaaaatc gaatcgagca gcgaacggac cccaaagtcg acctgcgagc
     3841 tgaccgcaag cgggccgttg gaagcgggcc cttgaattgg gcggtgcggc gcccgtttgg
     3901 acggtatagt gagcaggtgc cgcccgtctt tttgcagtcg tggggtccgc ttttggtggt
     3961 catccatgcg ctcagcgccg tcgtgctgtg cggtgccagc gttcaccaag cggtgctggc
     4021 gacgctgctg ctgctgaggc gggccgtgcg agggcggctt cttcgcgtct attcggtgac
     4081 gacgctattg tcctatctgg cgacggtgac aggcggggcg cttttgtatc cgcgctatcg
     4141 cgtcgtcatt cgggcgctgt ttctcgatca gaacgcgccg tgggccgcga acctgttcga
     4201 cttcaaagaa aatctcgcga cgctcggctt gccactcgcc gtgggcgcgc tgctgcttgc
     4261 gatgccgctt tcacagcgaa gcgcgcggac tttaccgaca cccggcgatg ttgtgacgga
     4321 cgcggaccca gcggcggatt ccgatgcagc ggcggattcg cagcggctgg cctatctgac
     4381 gttcttggtg atggcgctcg gcacggcgct cgtggcggtg atcaacttga tcgcggggct
     4441 tgtctgtacc gcggtgcgag gcgcctgatg ttgcctgacg ctcggcgtgc tgcgcttttt
     4501 gcctgctggt tttcggcggt gacatccgct gttttttttg tcaccggggt ggtcgcgctg
     4561 ccagtcccgt tgtatgtgcc gcttgtgcat cgctgggtga tgtcggcgtc cgccgcggaa
     4621 cagcttggtc cgctgggcag tctgacgatg gattattttg ggcggtcgct gctggctgtc
     4681 ggctgtggcg cggtgacgac ccttttgggg atgatttgtc tgcgtctgcg acaccgcaca
     4741 tcatcgctgg tcgatggaaa atcgtcgccg gggacattgc gcaagcctag ttcgatacag
     4801 cttcaactgg cgctggctta tgcagtgaca gcgtttttgc tgtgtgcggc gctgtttgcc
     4861 tacaagctgt acggtcgcga gccgtcttcg cccgttttgc ctgaccccag tccgatttct
     4921 gcggaccgtt tgccgtagcc aaaaaagacc gacaggacgt gcgtgttttt tgtcaatctg
     4981 actcggcaac cgcccaaagc ccggcgcgac cagttggcaa aaggcccgtc ggaagctggc
     5041 cccgtcgtcg ggtttttctg cctcggaaag aagcaattgc ggtaacttga gtaattaggc
     5101 tatattgacc gtggtggtta ccgggctttc acgagaggca gagcggggga tctgtcgcca
     5161 gagttcaggt cgccgtttct ccctttcctg cgctgcacgg tggtttgcgg cgggcgttgc
     5221 catgctgagc ttggcttcgg ctgaagcgtc gccccgcaaa gccggcctgc catcgaagct
     5281 gtcgctgttt tcgcgggtct tgtcctacgt cgaaagccag tacgtcgaag atgtcgatca
     5341 agacgagctg gtgtacggcg cgatcaaagg catgctcgac tcgctggacc cgcacagcac
     5401 gtttttgcgc cccgagcagt atcgcgagct gaaaaacgac acgagcgggc agtttgcggg
     5461 aattggaatc gaggtcgatc agcgcgacgg cctgctcacg gtggtgtcgg tgatagacgg
     5521 aacgccggcg gcgcgggcgg gacttttggc cggtgatcag atcctgcgca tcgacgacca
     5581 gccgactcgc gggatgatgg actatatcca aaagatgcgg ggcaagcgcg gcatccaggt
     5641 gacgctgacg gtgctgcgca gcggctggaa agatccgcgc aatttcgcga tgctgcgcga
     5701 catcatcaag attcgcagcg tcgaaggatt cttgcttgaa ccgggctacg gcgtgctcaa
     5761 ggtcaagcag tttacggaca acatcgaccg cgaagtcgat gcgcagctcc aggcgatgga
     5821 aaaagagtcg ggtggcaagc taagcgggct ggtcctcgat ttgcgcaaca atccgggggg
     5881 gctgctcgat caagcggtgc gactggcgga catctttatc gaaagcggcc tgatcgtgcg
     5941 gaccgaaggc cgtagcgggc ggattctcga cgaagaacgc gccagacctg aagggacgcg
     6001 gctcggtttt ccgatcatct gtttggtgaa cggcggatcg gcgtcggcag cggaaattgt
     6061 cgctggcgcg ctgcaagatc acggtcgcgc cctggtgatg ggcacccaga cctttggcaa
     6121 aggttcggtg cagaccatcg ttgaactgga agacggcagc gcgctcaagc tgacgattgc
     6181 gcgctactac acgccatcgg ggcgatcgat ccaagaaaag gggattaccc ctgacgtggt
     6241 ggtcgatcag gtccggctgg ctgatggcaa gctggtcgtg cgcgacgagc cgtcgcaaaa
     6301 agagcgcgac ttgcaaggtc atctcaaaaa tcgacagaag cagacggttg agtcgggccc
     6361 tccgcctggc tattccgtca tcgcgcagca cgcgccacaa ggtgaggact ttcagcttcg
     6421 aaccgcttac gattatttga aagcgtg
//
LOCUS       DAORGN010000249         6299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5929_length_6299_cov_6.536996, whole genome shotgun sequence.
ACCESSION   DAORGN010000249 DAORGN010000000
VERSION     DAORGN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_6299_cov_6.536996"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKL17_19355"
     CDS             <1..965
                     /locus_tag="PKL17_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNK46951.1"
                     /translation="TQNKRAVLSSDASMDVRFVHSQSIILQGIRSTMSVPLMHGSELL
                     GVMHLDSQIAANAFTERDLQLFNSIANQAAIAIQNARLTKKIEEEAKTRAQFQRLLSP
                     KLVEQLVSGKLHLEKGGELREVTMLFSDIRGFTAMTERHKATEIVAMLNEYFEVMVEI
                     LFKYNGTLDKYVGDEIIGLFGAPVPVEEATWCAVQCAIEMQTALQHFNSKRKPLGLEA
                     IDIGIGINIGSVVCGMIGSSHTMQYTVIGDAVNTASRLCSVAGKGEIIVSEQALQAVS
                     DRVEYVALPPTKVKGKLQALRIFNVLGRKGDATGFFTRDMTTPG"
     gene            complement(998..1363)
                     /locus_tag="PKL17_19360"
     CDS             complement(998..1363)
                     /locus_tag="PKL17_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46952.1"
                     /translation="MRFAARYLVGLVATGLLACQGRVGTPCQGASDCQSGLLCVKPPG
                     LGAGASGVCEQARRGVGESCAVSADCSSGLVCSNETAGGYYGMCQPAVYLDGGAKDMA
                     MDMSAQVDLTVQDLTTPPG"
     gene            complement(1367..3364)
                     /locus_tag="PKL17_19365"
     CDS             complement(1367..3364)
                     /locus_tag="PKL17_19365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HNK46953.1"
                     /translation="MRRPDPARLADPDFRRLCGSSELPTSSRRALTLGTAVLFVLLGV
                     TQSAHAQFGPGGGMRPMGGNGAGGMGGPQGGPPPDKPEGPAEAAPSTGTTDTATEQLP
                     PWPEKKGKELQFLQLNGYLRGRGYYWHNFNLGHYNDTATRANPFSIPYSEIPTSTGER
                     PASSCINRNPGSGCIQRGVLTSDMRLRFDPTLNLSEQIRVKSQIDVFDNLVLGSTPEG
                     FYINGRGGAADGYPTLLNRSQVPQESVVNALQSSIRAKRAWGEVKIPLAELAFGRMPF
                     MWGTGMLYHDGNCPDCDFGTTVDRVMITARLWNHFASFMYDWAAVGATSQIVNNQQLT
                     GWNYNIDNVDDVMQVGLALGRKDDDASIADALNRGKAVFNYGALVIGRWQEWDLTYNP
                     RPQTTPLTQTGPDSSALYSAQELQKSLGRRTAWSLTADLWGRLQYKKLYLEAEGAAIF
                     GSVGELGVYGDGGYGTDNVLRKSTLQIQQLGGLVRGNYKFLRDSLIVGLEVGSGSGPQ
                     NGDPRGELNFRRAREKPSGLLNTDGLINLRSSRFTFDPDYHVDMILFRRILGTVYNAT
                     YMKPSVTYWLIDSFGGQAEFIYSLANKPAVFPGHAINMGAEINARLMYHNKDEGFYAA
                     LEYGVLFDLGALSQKQEVWNNTFPVDGTTAQAFQAKIMLRF"
     gene            3731..4657
                     /locus_tag="PKL17_19370"
     CDS             3731..4657
                     /locus_tag="PKL17_19370"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNK46954.1"
                     /translation="MWTHRFAIFTACATFTLLLIGGIVHGTGSSLACPDWPLCFGQFF
                     PEMKGNVFFEHGHRLFASLVGILTVALCGFGYKATLPGKRRLFQLTLLALGLVIFQGV
                     LGGLTVIYKLPTLISLAHLCTSMLFFALVLTIACLSRPQPARDEVSRRAYRFLAATAI
                     LCYLQITLGGLVRHTGAGLACLEIPFCKSGLLPLGEHPTIVIQALHRLTAFLLTLAIV
                     GSSLMLPALKGVARQIKLALPMLVVVQIGLGLESVRSYLGLYQVTTHLGVGALLWALL
                     VVELWLLRPMAEGQEVSTEIPTALVSQQGGVA"
     gene            4654..5556
                     /gene="cyoE"
                     /locus_tag="PKL17_19375"
     CDS             4654..5556
                     /gene="cyoE"
                     /locus_tag="PKL17_19375"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNK46955.1"
                     /translation="MSQVRDIIALIKPRITLMVVATGAVGLYLAPSAPAPQTKLGLLV
                     GMTLLVGGANALNMYLERDIDGRAERTKDRPLPAGRLSPKLALGLSLTLGMLALLWLG
                     LLVNTLTAVLGLLAFVSYTAFYTPMKQYSPGALVVGAVPGAMPPLMGWTAATSQLDLA
                     GLTLFAIMFLWQLPHFIAIAIFRNDDYRRAGFKTLVSELGTTTAKFQVVLYLLLLCPV
                     SLLLVPMGIGGKLYLWTALVAGLGFLVVGMQGLRASAGVIWAKQLFKASLLYLMVVFG
                     AMLAGQAMQGGDAATLYAVGHPAD"
     gene            5566..5874
                     /locus_tag="PKL17_19380"
     CDS             5566..5874
                     /locus_tag="PKL17_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46956.1"
                     /translation="MKLRTLYEKILTNPKLVTMFSSFTPAAAEDRSPLVRTTLAVGTI
                     TVFGLALATATSALALMLVAVGVVYYLMTQILGVRIELDPQAILRQAMQTQRPPSAPN
                     "
     gene            5903..6220
                     /locus_tag="PKL17_19385"
     CDS             5903..6220
                     /locus_tag="PKL17_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNK46957.1"
                     /translation="MIPYERHILVCTNRRDPQNPKGSCAHSGGDGICDVFKKELHQRG
                     LKGRMRANSSGCLDQCSQGPVVVVYPDAVWYKVPTEVDALEIIDQHIVGGVPVERLRI
                     RSR"
BASE COUNT         1217 a         1951 c         1855 g         1276 t
ORIGIN      
        1 tgacgcagaa caagcgcgcg gtgctgtcgt cggacgccag catggacgtg cgctttgtgc
       61 acagccagtc gatcatcttg cagggcattc gctcgacgat gtcggtgccg ctgatgcacg
      121 gcagcgagct gctcggggtc atgcacctcg actcgcagat tgcagccaac gcatttaccg
      181 agcgtgacct tcagcttttc aactcgatcg caaaccaggc ggccatcgct attcaaaatg
      241 cccgtctgac caaaaagatc gaggaagaag ccaagacacg cgcgcagttc caacggcttc
      301 tgtccccgaa gctcgtcgag cagctcgtgt ccggcaagct ccacctcgaa aaaggcggcg
      361 agctgcgtga agtcacgatg ctgttttcgg atatccgcgg ctttaccgcg atgaccgaac
      421 ggcacaaagc gaccgagatc gtcgccatgc tcaacgaata cttcgaggtg atggtcgaga
      481 tcctgtttaa gtacaacggc acgctcgaca aatacgtcgg cgacgagatc atcggcctgt
      541 ttggcgcacc agtccccgtc gaagaagcaa cgtggtgcgc ggtccagtgt gcgatcgaga
      601 tgcagacggc gcttcagcac ttcaactcga agcgcaagcc gctcgggctc gaagcgatcg
      661 acattggcat cggcatcaac atcggatcgg tcgtctgcgg catgatcggc tcgtcgcaca
      721 cgatgcagta caccgtcatc ggcgacgctg tgaacaccgc atcgcgactg tgttccgtcg
      781 ctggcaaggg cgaaatcatc gtcagcgaac aggccctgca agccgtgagc gatcgcgtcg
      841 aatatgttgc gctgccaccg acgaaggtaa agggcaagct gcaagcgctc cggatcttca
      901 acgtcctcgg acgcaagggc gacgcgacgg gctttttcac ccgcgacatg accacgcccg
      961 gctgatccgc gactgccagc gcagccgcgg gtttttgtca gcctggcggc gtcgtcaaat
     1021 cctgcaccgt caagtccacc tgcgcagaca tgtccatcgc catgtctttc gccccaccat
     1081 cgagatagac cgccggttgg cacattccgt agtagccccc cgccgtctca tttgagcaga
     1141 ccagccccga gctacagtcc gcagaaacgg cacaggactc accgacacct cgtcgcgctt
     1201 gctcacagac cccggaagcc ccggccccca aacccggtgg cttcacacaa agaagtccgc
     1261 tctggcagtc actcgcgcct tggcacggcg tccccacccg cccctggcaa gccagcaggc
     1321 ccgtggcgac aagcccgacg agatatcgcg cagcgaaacg catgagctag aagcggagca
     1381 tgatcttggc ttggaaggcc tgcgccgtcg taccgtcgac ggggaacgtg ttgttccaga
     1441 cttcctgctt ctgcgacagg gcccccaagt cgaacagcac accgtattcg agcgccgcgt
     1501 agaagccctc gtctttgttg tgatacatga gccgcgcgtt gatttcggcg cccatgttga
     1561 tcgcgtgacc agggaacacc gccggtttgt tggccagcga gtagataaac tccgcctgtc
     1621 caccaaagct gtcgatcagc cagtacgtca ccgacggctt catatacgtc gcattgtaga
     1681 ccgtcccgag aatgcgccgg aacaggatca tgtcgacgtg atagtccggg tcaaacgtaa
     1741 accgcgagct acgcaggttg ataagtccgt cggtgttcaa aagtcccgac ggcttctcgc
     1801 gcgcccgccg gaagtttagc tcgccgcgcg gatcgccgtt ttgcggcccg ctgccactgc
     1861 cgacttccag accgacgatc aggctgtcgc gcagaaactt gtagttgccg cggacaagac
     1921 cacccagctg ctggatctga agcgtcgact tgcgcaacac gttgtccgtg ccgtatccgc
     1981 catcgccgta aacgcccagc tcaccaacgc tgccaaagat cgcggcgcct tccgcttcca
     2041 gatacagctt tttgtactgg agccggcccc acaggtctgc ggtcagcgac cacgcggtgc
     2101 gccgaccgag cgacttttgc agttcttgcg cggaatacag cgccgaagaa tcaggccctg
     2161 tttgagtaag cggcgtggtc tgcggacgcg ggttgtaggt caaatcccat tcttgccagc
     2221 gtccgatcac cagcgcgccg tagttaaaca ccgctttgcc acggttgagc gcgtcagcaa
     2281 tcgaggcatc atcgtctttg cgcccaagag ccagaccgac ctgcatcaca tcatcgacgt
     2341 tgtcgatgtt gtaattccag ccggtaagct gctggttatt gacgatctgc gacgtcgcac
     2401 cgacggcagc ccagtcatac ataaacgacg caaagtgatt ccacagccgc gccgtgatca
     2461 tcacgcgatc gaccgtcgta ccaaagtcgc agtccgggca gttgccgtcg tggtagagca
     2521 ttccagtgcc ccacataaac ggcatccgac caaacgctag ctcggcgagc ggtatcttga
     2581 cttcgcccca cgcacgcttg gcgcggatcg acgactgaag cgcatttacc accgattctt
     2641 gcggtacttg actgcgattc aggagcgtcg ggtagccatc tgctgcacca ccgcgcccgt
     2701 tgatgtagaa gccttccggc gtgctcccga gcacgaggtt gtcgaagaca tcgatctgcg
     2761 acttgacccg gatctgttcc gacagattca gcgtggggtc aaagcgcaac cgcatgtccg
     2821 acgtcagcac accgcgctgg atgcagccag aacccggatt gcggttgatg cagctcgacg
     2881 ctggtcgctc acccgtcgac gtcggaattt ccgaatacgg gatcgaaaat ggattcgccc
     2941 gcgtcgccgt gtcgttgtag tgaccgaggt tgaagttgtg ccagtagtag ccgcggcctc
     3001 gcagatagcc gttgagctga aggaactgga gttccttgcc ctttttttcc ggccacggtg
     3061 gaagctgttc agtcgcggta tcagtggttc ccgtcgaggg agcagcttcg gctggacctt
     3121 ctggcttgtc aggaggagga ccgccctgtg gaccgcccat tccccccgcg ccattgcccc
     3181 ccatgggccg catcccgcca ccgggaccaa actgggcatg tgccgattga gtaacgccaa
     3241 gtaggacgaa gagcacggca gtgcccagcg tcaaggctcg tcgcgagctt gtgggaagct
     3301 ctgacgagcc acagaggcga cgaaaatccg ggtcggcgag ccgagccggg tcaggccgtc
     3361 gcatgaagac ctccgtgttc tttcgatgga agcggtgaga gcgcaagatt gcgtggcgga
     3421 ctatagagcc gcgcatctgg gaggtcaacc tcccaatccg aacttcgata cgccggcacg
     3481 acgacacgct ttctcgcgag ccggtttcaa tcaaaacggc gacgctacgg cctcgtggtg
     3541 gccgcaaatg aatcgatcac tggccgcgcg tccgtcggta tcagcagatt cacaaaaaaa
     3601 cgcttggctc cgctcggtcg cttgccgtaa agacgcgagg taacgtcagc atttacccac
     3661 gtagacgagc gccgcacggc atacgcgctg cagattttcg acggtgttcg tccttcgagg
     3721 catgaccccc atgtggaccc atcgctttgc catctttact gcctgtgcca cgttcacttt
     3781 gctgctcatc ggcggcatcg tacacggcac cggttcgtcg ctcgcctgcc ccgactggcc
     3841 gctttgtttt ggtcagtttt ttccggaaat gaaaggcaac gtgttcttcg agcacggcca
     3901 ccggctgttc gcaagtctgg tcgggatttt gactgtggcc ctgtgtggat tcggctacaa
     3961 ggcgacgctg cctggcaagc gacggctgtt ccagctgacg ctgcttgcgc tgggcctggt
     4021 gattttccaa ggcgtgctgg gtggtttgac ggtgatttac aaattgccga cgctgatctc
     4081 tctagctcac ctttgcacgt cgatgctgtt ctttgcgctg gtgctgacga ttgcgtgcct
     4141 ctcgcgaccg cagcccgcac gcgatgaagt ttctcgtcgc gcgtaccgat tcctcgcagc
     4201 gacagcgatc ctctgttacc tccagatcac gctcggcggc ctggttcgac acacgggagc
     4261 cggtctcgcc tgcctcgaaa tcccgttctg caaaagtggc ctgctgccgc tcggggaaca
     4321 tccgacgatc gtgatccagg cgctgcatcg gctgactgcc tttttgctca cgctggcgat
     4381 cgttggttcg tcgctgatgt tgccagcgct gaaaggcgtt gctcgtcaga tcaagctggc
     4441 actgccgatg ctggtggtgg ttcagatcgg ccttggtcta gaaagcgtgc gaagctatct
     4501 tgggctgtat caagtgacca cgcacctcgg tgtcggcgcg ctcttgtggg cgcttttggt
     4561 ggttgagctg tggcttctgc gaccgatggc cgaaggccaa gaagtgtcga cggaaatccc
     4621 gaccgccctc gtcagccagc agggaggcgt cgcttgagtc aggtccgcga catcatcgcg
     4681 ctcatcaagc cacggattac cctgatggtg gtggcgacgg gtgcagtggg tctgtacttg
     4741 gcgccaagcg cgccagcgcc gcagaccaag ctagggctgc tcgttgggat gacgcttctc
     4801 gtcgggggag ccaacgcgct caacatgtac ttggaacgag acatcgacgg acgagccgag
     4861 cgaaccaagg accgaccgct accggctggg cgactctctc ccaagctggc gctcggactg
     4921 tccctcacac tcgggatgct cgcgcttttg tggcttggac tgctcgtcaa cacgctgacg
     4981 gcggtgctgg ggcttctcgc ctttgtgtcg tacaccgcgt tttatacccc gatgaaacag
     5041 tattctccgg gggcgctcgt cgtcggagca gtgccaggtg ccatgccacc acttatgggc
     5101 tggacggcgg cgacgagcca gctcgacctc gcaggactga cgctgtttgc gatcatgttc
     5161 ctgtggcagc tgccgcactt tatcgcgatc gccatttttc ggaacgatga ctaccgtcga
     5221 gccggattca agacgctggt gagtgaacta ggtacgacga cggcaaagtt tcaagtcgtg
     5281 ctctatctgc ttttgctctg cccggtctcg ctgctgctcg ttcccatggg catcggcggg
     5341 aagctgtatt tgtggacagc actcgtcgca ggactcggat tcttggtggt cggcatgcag
     5401 gggctgcggg catccgctgg cgtcatctgg gccaaacagc tattcaaagc gtccttgctg
     5461 tatctgatgg tggttttcgg cgcgatgctc gccggacagg ccatgcaggg cggcgatgcg
     5521 gccacgctct atgccgtcgg acacccagca gattaggaaa tcgcgatgaa gctccgaacc
     5581 ctctacgaaa aaatcctgac caatcccaag ctggtgacga tgttttcgtc gttcacacca
     5641 gccgccgccg aggatcgcag cccgctggtt cgcacgacgc tggcggttgg aacgatcacg
     5701 gttttcggtt tggcgctcgc gacggccacg tctgccctgg cgttgatgct ggtcgccgtc
     5761 ggagtcgtct actacttgat gacgcagatt ctcggtgtcc gcattgaact tgatccccag
     5821 gcaatcctgc gacaagccat gcagacgcag cgaccgccgt cggcgccgaa ttagtccata
     5881 cacctcacac cgggcggaaa cgatgattcc ttacgaacgg cacatcttgg tctgcaccaa
     5941 tcgccgcgat ccgcaaaatc ccaaaggaag ctgcgcgcac agcggcggcg acggcatttg
     6001 cgacgtgttc aagaaagaac tgcaccagcg cggactcaag ggacgaatgc gcgccaactc
     6061 atccggttgt ctcgatcagt gcagccaggg ccctgtggtg gtcgtgtatc ccgacgccgt
     6121 ttggtacaaa gtcccgactg aagtggacgc cctagagatc atcgatcagc acatcgtcgg
     6181 cggggtcccc gtcgagcgac tacgcattcg ttcgcgctag gggccgtccc taattcccgc
     6241 agactgccga cggtagcggg ctcggcagca gaacttgtgc gtcgcggcaa agcatgatc
//
LOCUS       DAORGN010000250         6283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_5955_length_6283_cov_6.003372, whole genome shotgun sequence.
ACCESSION   DAORGN010000250 DAORGN010000000
VERSION     DAORGN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5955_length_6283_cov_6.003372"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..531
                     /gene="tssE"
                     /locus_tag="PKL17_19390"
     CDS             112..531
                     /gene="tssE"
                     /locus_tag="PKL17_19390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNK46958.1"
                     /translation="MPPKATSCRSLFGRLRGPSPSDASLSPSDALRESILRNLERILN
                     SRAGMSLACETYGIPDLTQIVSGVPERARDIERAIEKCIREFEPRLTTVEVEHFPDSK
                     GPMTACFKIRASVSQGKSAEELWFETVVVSSGRVKLI"
     gene            539..2425
                     /gene="tssF"
                     /locus_tag="PKL17_19395"
     CDS             539..2425
                     /gene="tssF"
                     /locus_tag="PKL17_19395"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNK46959.1"
                     /translation="MSQASTFKRYYEDELAFLRELGGEFSHSYPDIARELGLQGHDPD
                     VERLLQGVAFLTGRIRQSLAAQFPDLLYPLLSHVWPQALRPTPCTAMIEFLPRPNMFR
                     EPFHLARGAELRAEPIDGTSCTFRTAYDTTILPLQIDDVRLSAPTVNRQRLTVCMKAL
                     PGAQLGRLVDETLRLHFHGSSVKGMGREAFGLYASIGHGLRFLKLVAQRADGSVLGER
                     TLLHDALQAVGFSSRETLLPYPVYSPSSYRLLTEFFLQPAKHLFFDVRALHVLADFPT
                     AERLELQLDFDPLPGEPLSPALLKECVRLNCVPAVNLFPHTASPLLVDGKRTEYPLRP
                     EGTAPHHHEIFSVEKVMGHLDRQPNPTEYFPFYSFHRPKSKLGEVPVLYQVIPRAPVA
                     ETRSDHEGLSTHPAVPMYLALVMADGRPVVFSDDEKALPTNVSAKQAVVSVDLLCTNR
                     DLPTRLRTGSICRPTSDIPSTLQFRNIGPISEPAPALVTSGGDGLWRLFAHQLMAQNH
                     FRDRDALRMLFQLFDVRAQVNQAARHKLETLLDAVIEVSAQAEFRLAGSPKAVTRGTH
                     VELRLHEDRFAFLGELYLLGDVLEQFLAESSQVNTFTALSLHGKDHDLTIHFPARLGH
                     KRLL"
     gene            2422..3429
                     /gene="tssG"
                     /locus_tag="PKL17_19400"
     CDS             2422..3429
                     /gene="tssG"
                     /locus_tag="PKL17_19400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNK46960.1"
                     /translation="MSGADSVAGYQLFSLLRLLESQAPQAAALGESGPASAEAVRLRP
                     SLELSFPTSEAVAVEHRQSVDGAPRSVVTANISGLYGVGSPLPRSYPHQVLLQADTDP
                     QQRDFLDIIHHRVLSLWYRSFRRQHYEQSFAEDGTDLLSQALLDWIGVPRGATEDQLG
                     TSPLRLLRYLGLFAASTRTAAGLEVLLREELHQPIVVEPASLRWVRLPKSQWPTLSRN
                     PKQRAALGRDLVIGSRHLDRQCSLTLHMGPVSYETLLAFWPGETLHTRLHALASFYLR
                     QPLALTLRVSVPKSELARQRLGRQSGGMLGRPAAMGEPQTDPVVLVIPDRENRGCTES
                     A"
     gene            3443..6202
                     /gene="tssH"
                     /locus_tag="PKL17_19405"
     CDS             3443..6202
                     /gene="tssH"
                     /locus_tag="PKL17_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HNK46961.1"
                     /translation="MFLVEYRALLKRLNPLCTKALEAAASLAISRSHFEIGVEHFLAK
                     ILEEPSGDASAIFRALSVSGTPLLRAIHRGSDQRKRGNSGRPVFSPILVSLIQDAWLL
                     SSVEGGDNKIRSGYLLLTLLLQPERYGAAEYAELLSVIDRDALRRRFAELTAGSSEIP
                     LTAPEAEAGEARSTASPAYAAAAEDSALGKFTYDLTARARAGKIDPVLGRDREIRQLL
                     DILGRRQKNNPIIVGESGVGKTALVEGLALRIADKDVPPSLLDVEVRSLDMGLLQAGA
                     GVKGEFEARLTAVIGEVKASPRPIILFIDEAHTMIGAGASAGGSDAANLLKPALARGE
                     LRTIAATTWSEYKKYFEKDAALSRRFQPVKVEEPSREVAIDMLRGLAVKLQAAHGVHI
                     CDEAVVTAVDLSRRYISGRLLPDKAVDLLDTCAARVKLGLSTRPAELEALSRTVESID
                     RERSARREDLHLSGQSALSHPSLGELSEALAMAEQKRDELAARWQAESQVAQEILTLR
                     QKLAQKDKLTHDAHAAVHTLPVTTQGQQPQPGETVAAENTEVLAQALAEALPKLRALQ
                     QEIPLVTVEVDPETVAKVVADWTGIPVGKMVRDDVAATASIEDRLMKRIRGQDAAICA
                     IARRLRNAKAGLQDPRQPLGVFLLVGPSGTGKTETALSVADLLFGGERFMVTINMSEF
                     QEKHTVSRLIGSPPGYVGYGEGGVLTEAVRQRPYSVVLLDEVEKADPEVLNLFYQVFD
                     KGMLADGEGRVVDFRNTVIFMTSNLASDVTMRLAAEGTLTPSVEELGKALRPALNRHF
                     KPALLARMTVVPYFPISTDVLHDIISLKLGHVAARMLDSHGSTLRFAEQVVAAIASRC
                     QEAETGARNADHIIREHLLPLLSSYVLQHVVEGDLPSAIAVILDAAGHFTVAEESESA
                     VAQAG"
BASE COUNT         1237 a         1873 c         1912 g         1261 t
ORIGIN      
        1 acgaacaagg cggaatcggc gccgaagccg actggcgcga accgccggtg tagtccaaat
       61 cgacaaagtg ggggggacaa gatcgcacag ctcggcattt agaaaaaagc catgccccca
      121 aaagccacat cttgtcggag cctgtttggg cgcttgcggg gcccctcccc ttctgatgcg
      181 tcgctgtcgc cgtcggatgc gctgcgcgag tccattctgc gcaacttgga gcgcatcctc
      241 aacagccggg cggggatgtc gctggcatgt gaaacgtatg gaattcccga cctaacccag
      301 attgtcagcg gcgtaccaga acgagcgcgt gacatcgagc gcgccatcga aaagtgcatt
      361 cgcgagtttg aaccacgact caccactgtg gaggtcgaac atttccccga ttcaaaggga
      421 ccgatgacag cttgttttaa aatcagagca tccgtttccc aaggcaaaag cgcggaagaa
      481 ctgtggtttg aaacggtagt ggtttcgtcg gggcgagtga aactcatata aggcattcat
      541 gtcacaagca tccacattta aacgctatta tgaagacgaa ttggcatttt tgcgcgaact
      601 gggtggcgag ttttcccatt cttatcccga tatcgcacgc gagctgggac tgcaaggtca
      661 cgatcccgat gtcgagcgac ttttgcaagg agtggcgttt ctgaccggac ggattcgcca
      721 gagcctcgcg gcacagtttc ccgatctgct gtatccgctg ctttcgcatg tctggccgca
      781 agcgctacgc ccgacgccat gcaccgcgat gatcgaattt ttgccgcgtc cgaatatgtt
      841 ccgcgaaccg ttccatctgg cgcgcggcgc ggagctgcga gccgagccca tcgacggcac
      901 aagctgcacc ttccgaacgg cgtatgacac gacgatcttg ccgctccaga tcgacgacgt
      961 tcggctgtcg gcccccacgg taaatcgcca gcgtttgacg gtctgcatga aagcacttcc
     1021 gggggcacag ctcggtcgtc tggtcgacga aacgctgcgg ctgcactttc atggttcgtc
     1081 cgtcaaagga atgggccgtg aagcgtttgg actgtatgcg agcatcggtc acggactgcg
     1141 tttcctcaag ctggtggcgc agcgggccga cggaagcgtc ctgggtgaac ggacactttt
     1201 gcacgatgcg ctccaagcgg tgggtttttc aagtcgcgag accctgcttc cgtatccggt
     1261 gtacagcccc agcagctatc ggctgctgac ggaatttttc ttgcagccgg ccaagcacct
     1321 gtttttcgat gtgcgcgcgc tgcatgtgct ggcggatttt ccgacggcag agcgcttgga
     1381 actccagctt gatttcgacc cgctcccagg tgaaccgctg agtccggcgc tgctcaagga
     1441 gtgcgtccgg ctgaactgtg ttccggcggt caacttgttt ccgcacaccg caagcccgct
     1501 tttggtcgat ggaaaacgga cggaataccc gctgcgtccc gaaggaaccg cgccgcatca
     1561 ccatgaaatt ttcagcgtcg agaaagtcat gggacacctc gaccgccagc cgaatccgac
     1621 cgagtatttt ccgttttact cattccatcg gcccaagtcc aagctcggcg aagttccggt
     1681 tctgtaccaa gtgattccgc gagcgcccgt cgctgagacg cgaagtgatc acgaaggact
     1741 gtcgacccat ccggcggttc ctatgtatct ggcgctggtg atggcggacg gcaggccggt
     1801 ggtcttttcc gacgatgaaa aagcgctgcc taccaacgtg tctgccaaac aagcggtggt
     1861 ttctgtggat ctgctgtgca caaatcgcga tctgccgacg cgactgcgca ccgggtccat
     1921 ttgtcgaccg acaagcgaca ttccgtcgac gcttcagttt cgcaacatcg gaccgatatc
     1981 ggaaccggcg cccgcgcttg tgacaagtgg cggcgatggg ctgtggcgac tgtttgcgca
     2041 tcagctgatg gcgcagaatc acttccgcga ccgcgacgca ctgcgcatgc tgtttcaact
     2101 gttcgatgtg cgcgcccagg taaaccaggc ggcgcggcac aaactggaaa cgctgctcga
     2161 cgcggtgatc gaggtgagcg cgcaggcaga attccgactg gccggatcgc ccaaagccgt
     2221 gacgcgcggc acccacgtcg agctgcgcct gcatgaagac cgctttgcat ttttgggtga
     2281 gctgtatctg ttgggcgatg tgctcgagca gttcctcgcg gagtcgtccc aggtcaacac
     2341 gtttacggcg ctgtcgctgc atggcaaaga ccacgacttg acgattcatt tcccagcccg
     2401 gctcggtcac aagaggctgc tatgagcgga gcagattccg tcgctggcta tcagcttttt
     2461 tcactgctgc gactgcttga atcacaagcc ccacaagccg cagcgttggg tgaaagtggc
     2521 cccgcgtccg ccgaagcggt ccgtctgcgt ccgtcactgg aactgtcttt cccgaccagc
     2581 gaagccgtcg cggtcgaaca ccggcaaagt gtagacggag cgccccgttc cgtcgtcacc
     2641 gcgaacatct cgggactgta tggagtgggt tcgccgctgc cgcgctcgta tccgcatcaa
     2701 gtgctactcc aggccgatac cgatccgcag cagcgtgact ttctcgacat cattcatcat
     2761 cgcgtgctgt cgctttggta tcgttcgttc cgacggcaac actacgaaca gtcgtttgcg
     2821 gaagacggca ccgatctgct ctcgcaagcg ctgctggatt ggatcggtgt cccgcgcggg
     2881 gcaacggaag atcagctcgg aacatcgccg ctccgcttgc tgcgctatct gggactgttt
     2941 gcggcaagca cccgcaccgc agccggtctg gaagtgcttt tgcgcgaaga actccatcag
     3001 ccgattgtgg tggagcccgc ttcgctgcgc tgggttcgtc tgcccaagtc acagtggccg
     3061 acgctgtcac gaaatccgaa acagcgggcc gcgctcggac gtgatttggt gatcggctcg
     3121 cgccatctgg accggcagtg ctcgctgacg cttcacatgg gtccggtttc ctatgaaacg
     3181 ctgctcgcat tttggcccgg tgagacgctg cacacgcgac tgcatgcact ggccagtttc
     3241 tatctccgcc agccgctcgc gctgacgctc cgtgtgtcgg ttcccaaatc agagctggcc
     3301 cgccaacggc tcggtcgtca gtcgggcggc atgctgggac gccccgccgc catgggagag
     3361 ccgcagacgg acccagtggt gctggtcatt cccgatcgcg aaaaccgcgg atgtacggag
     3421 tccgcttaga aagggtattg tcatgtttct cgtcgaatac cgcgcacttc tgaagcgact
     3481 aaacccgctt tgtaccaaag cactggaagc cgccgcgagt ctggcaattt cccgttcgca
     3541 cttcgaaatc ggcgtcgaac attttctggc caaaattctg gaggagccat ccggcgacgc
     3601 cagcgccatc ttccgcgcgc tgtctgtttc tggcacgccg ctcttgcgcg cgattcatcg
     3661 cggatctgat cagcgcaagc gcggcaacag cggtcgtccg gtcttttcgc ccattttggt
     3721 ttcgctgatc caagatgcgt ggcttttgtc gtcggtggag ggcggggaca ataagatacg
     3781 ttctggatac ctcttattga cgcttttgct ccagccggaa cgctatggtg cggcagagta
     3841 tgcagagctg ctttctgtga tcgatcgcga tgcgctgcgg cgtcggtttg ccgagctgac
     3901 cgccggttcg tcggagattc cgctgactgc gccggaagca gaggccgggg aagcgaggtc
     3961 aactgcaagt cccgcctatg cagccgctgc ggaagacagt gcgctcggca agttcacgta
     4021 tgatctgacg gcccgcgccc gcgccggaaa gattgatcca gtcctgggtc gcgatcgcga
     4081 aattcgtcag ctgctcgaca tcctcgggcg gaggcagaaa aacaatccga tcatcgttgg
     4141 agagtcgggc gttggcaaga ccgctctcgt cgaaggactg gccctgcgca ttgccgacaa
     4201 ggatgttccg ccgtcgctgc ttgatgtgga agtccgcagc ctcgacatgg gacttttgca
     4261 agccggcgcc ggtgtcaaag gggaatttga agcacgtctg accgcagtga ttggcgaagt
     4321 caaggcatcg ccgcgcccga tcattctgtt tatcgacgaa gcccacacga tgatcggagc
     4381 gggtgcaagc gccggtggaa gcgatgccgc caacctgctc aagccagcgc tggcgcgcgg
     4441 agagcttcgg acgatcgcgg cgacgacctg gtcggaatac aaaaagtact ttgaaaaaga
     4501 cgcggcctta agtcgtcgct ttcagcccgt caaagtggaa gagccgtcgc gagaagtggc
     4561 gattgacatg ctgcgcggac tcgcggtcaa actccaggcg gcacatggcg ttcacatctg
     4621 cgatgaagcg gtggtgactg ccgtggatct gtcgcgtcgg tatatttcgg gacggctttt
     4681 gccagacaaa gcggtcgatc tgctcgacac ctgcgcggcc cgcgtcaagc tgggtctgtc
     4741 gactcgtcct gccgagctgg aagcgctgtc acggacggta gaatccatcg atcgcgagcg
     4801 cagcgcccgt cgcgaagacc tgcatttgtc gggacagtcg gcgctgtccc atccatcgct
     4861 tggcgagctg tctgaagcgc tggcgatggc agagcagaag cgggacgagc tcgcagcacg
     4921 ctggcaagca gagtcccaag tcgcgcaaga aatcctgacg ttgcggcaaa agctcgcgca
     4981 aaaagacaag ctgacccatg acgcccacgc agcggttcac accctgccgg tgaccacaca
     5041 aggacagcag cctcagccag gcgagaccgt cgctgcggaa aacacagaag tgctcgcgca
     5101 ggcgcttgcc gaggcactgc ccaagctgcg cgcactgcaa caagagattc cgctggtcac
     5161 cgtggaagtc gaccccgaaa cggtcgcgaa agtggtggcg gactggaccg ggattccggt
     5221 cggcaagatg gtccgtgatg atgtcgcagc gacggcgtct atcgaagatc gattgatgaa
     5281 gcggattcgc ggacaagatg cagcgatttg tgcgattgcg cgacgactcc gcaatgccaa
     5341 agcagggctg caagatccgc gacaaccgct tggcgtgttc ctgctcgtcg ggccgagcgg
     5401 tacgggcaag accgaaacgg cgctgtcggt ggccgacctg ctgttcggtg gcgagcgctt
     5461 catggtcacg atcaacatgt cggagtttca ggaaaagcac acggtctcgc ggctcatcgg
     5521 atcaccgccc ggctatgtgg gttacggtga aggcggcgtg ctcaccgagg cggtgcggca
     5581 gcgtccgtac tcggtggtgc tgctcgatga agtcgaaaag gccgatcccg aagtgctgaa
     5641 cttgttttat caggtcttcg acaaaggcat gctcgccgat ggagaaggcc gggtggtcga
     5701 tttccgcaac acggtgattt tcatgacgtc gaacctcgcg tccgatgtca cgatgcgact
     5761 cgccgccgaa ggcacgctga ccccaagtgt cgaagagctg ggcaaggcgc tccgtcccgc
     5821 gctcaatcgt cacttcaagc cggcgctgct tgcccggatg accgtggtcc cctactttcc
     5881 gatctcgacc gacgtgctgc acgacatcat ctcgctcaag ctgggtcatg tcgcagcgcg
     5941 catgctcgac agccatggat cgacgctgcg gtttgccgaa caagtggtgg cggcgattgc
     6001 atcgcgttgt caggaagccg aaaccggcgc ccgcaacgcc gatcacatca tccgcgaaca
     6061 tcttttgccg ctgctatcca gctacgttct ccagcacgtt gtggaagggg atctacccag
     6121 cgccatcgcg gtgattctcg acgccgccgg ccacttcacc gtcgccgagg aaagcgaatc
     6181 tgccgtggca caagcgggat gaccggggac aaaagggggc gttagttgat cgcacgaaac
     6241 gcatacagat agacgtcaca cttgctgctc gccgtacact cgc
//
LOCUS       DAORGN010000251         6244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6003_length_6244_cov_6.073841, whole genome shotgun sequence.
ACCESSION   DAORGN010000251 DAORGN010000000
VERSION     DAORGN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6003_length_6244_cov_6.073841"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKL17_19410"
     CDS             complement(<1..310)
                     /locus_tag="PKL17_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46962.1"
                     /translation="MKLQFELLVAARYLRADRPAGALDHLRWATFGLVVLTASVFAGS
                     ALVEYLLRDHLSETLWNLRGPLRAAKYLTVLLTLLVGFFIELIRRLTIFTTISTFGLF
                     L"
     gene            complement(307..1926)
                     /gene="lysS"
                     /locus_tag="PKL17_19415"
     CDS             complement(307..1926)
                     /gene="lysS"
                     /locus_tag="PKL17_19415"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNK46963.1"
                     /translation="MTQKPTSPAAKTAPSQTASPAASSNSDLEGQMEERRRAIASLRS
                     QGVNPFANDVSPTHQIFDLPASQPEDVAKLPHENELTADAPRYCVAGRLLQVNEMGKA
                     KFLFIRGDRGAMLQLYLKADEAESFALSNQLQLGDFVLSSGPLFKTRKEKRALRVEKL
                     RLLSKAVRPLPGKALQDGPKVSDEDWRYRQRYADMIVHPEVADVFRKRALIVREIRRF
                     FDDRGYVECDTRMLLSTNGGAAARPFRTHHNALDLDLYLRIATELDLKRLVVGGIDRV
                     YEIGRIFRNEGLSRFHNPEFTSIEFYQAYATYEDLMDLTEQLIERVCQAVNGSTTIDY
                     QEHKGVNLCHPFRRATMRQLVKEARPDAPIADGPCSDAEYAALPGYAKSVLGEKAPTG
                     YGHALVALFEEFVEKSLVQPTFVCQFPTEVSPLSRKNDRDPRFVDRFEFFCIGKEFAN
                     AFSELNDPDDQRSRFTDQVASKAHGDQEAMDYDEDFCRALEYGMPPTAGEGIGIDRLV
                     MLLTNSASIRDVIPFPQLRPELVAPSESESK"
     gene            complement(1934..2470)
                     /locus_tag="PKL17_19420"
     CDS             complement(1934..2470)
                     /locus_tag="PKL17_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK46964.1"
                     /translation="MILHLSKLTLRPFVAGDVESLARHANNRHIWLNLRDGFPHPYTV
                     KDAERWVAHAASLPAPQHVFAIVIDEHAVGGIGVHRLDKDPVYRDTAEIGYWLAEPYW
                     GRGVMTEAVKAVTRYAFDELSLFRVEAGVFAWNVSSARVLEKCGYSFEGRLRKQAFKD
                     GQHLDVMLYSRLRTDEVE"
     gene            2527..4188
                     /gene="murJ"
                     /locus_tag="PKL17_19425"
     CDS             2527..4188
                     /gene="murJ"
                     /locus_tag="PKL17_19425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNK46965.1"
                     /translation="MSDSASPAPSTPPSAASGKSKMAQTALLLSLATLFSRVLGLVRE
                     QVFAALLGAGFFGDAFTIAFRLPNLLRDLFAEGALSAAFQPAFQAARKQEGHAAAQRL
                     AQLVATCLIVVVGLITLLGVWFSPEMVGHWAQGFASTPGKAELTVLLTRIMMPFLLLV
                     SLAALAMGMLNAEQRFTPPALAPALFNLATVATGLGLWFVGIGRSKTAAVAWAVATLC
                     GGGLQLGLQLVPLWRSGYRFRLAWDLSDPRLKAVLWAMAPATIGLMATQVNIYISSRF
                     ASTEPGAASWLYYAFRLLQMPIGMFGVAVGTVALQRAADAVSEADFRVAMDGVRDTLH
                     RGLRLVTFYSLPTAVAFFVLAEPIIAIIYQRGAFSAEDTVATAEALKYYSLGLLFYSA
                     VKVVVPIFYSLKLARFAVMSTVLTVTGSVLVNLFLHPRFGFRILALSTSIGAAINFLF
                     LVVVFRARYGQLFSQTLQLGLLRMLVASVAMGAVLWILTPLLLGDAAHAAAYSQGPQH
                     VSLLRALLGFSVLCGIGGGSYAFLGWLFRLEEVDHVLSAVRRRIR"
     gene            4415..5239
                     /locus_tag="PKL17_19430"
     CDS             4415..5239
                     /locus_tag="PKL17_19430"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HNK46966.1"
                     /translation="MGALFLFGCGDSGTTGNSADGSQSGDSSGSSGSDGSVVLSGGFA
                     VASGAFLTGKDYGNPFASGIGTTCTAYVSSTKGPCHSNTYLRKDISDGLVGLPTRFEL
                     MVVDASCKPVPNAIVEVWYASPAGTYSKAAQTIDAGTGYGGSLSDLNTSFCTGNDATA
                     LASNWLRGYQISDASGRVTIDGIFPGWYSGRTSHVHFIVTANGKTYVTSQLLFDETLT
                     TAVYTKHQSYSARGNKDTTNAKDNVVNMGLPVASSIMSFSQQNDGALVCTKAITIA"
     gene            5334..>6244
                     /locus_tag="PKL17_19435"
     CDS             5334..>6244
                     /locus_tag="PKL17_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46967.1"
                     /translation="MDRATGLAGLGVLLLAIYPLSAKGLEPILPSRVCQALVDQKPIS
                     ECFAAIKQVPVDPLAAGACLRLKVNYPVSMCMRTIAGKRYSPAEISECDDLEDVWETI
                     GCFYQNGQRVAPRNYSGTYADQICPKLQSSRLIRLCVAAFQESYIQPSAALACDRIKG
                     AKQTLDCLTAIMNQRYSEAEVAACDGLATSTETIACFSREVASPDEAVPPPVYSQSDA
                     PPMYPRPQRSAEPGGCNSYGCYGPGGGCNSYGCFGFGGGCNSYGCWKNGGGCNSYGCY
                     GMAGGCNSYGCWKNGGGCNSYGCWHSR"
BASE COUNT         1207 a         1851 c         1858 g         1328 t
ORIGIN      
        1 ccagaaacaa cccgaacgtc gatatcgtcg taaagatcgt cagccgccga atcagttcga
       61 tgaaaaaacc gacgagcagg gtcagcagca ccgtcaggta tttggccgcg cgcaaaggtc
      121 cgcgcaggtt ccacagcgtt tccgacaggt gatcgcgcag caggtattcg accagcgccg
      181 atccagcaaa caccgacgca gtaagcacaa cgagcccaaa cgtcgcccag cgcaagtgat
      241 cgagcgcccc cgcaggccga tccgcccgca ggtagcgcgc cgcaaccagc agctcgaact
      301 gaagcttcac ttgctctcgc tctcgctcgg tgccacaagc tcgggacgaa gctgcgggaa
      361 gggaatcacg tcgcgaatcg aagcactgtt ggtgagcagc atgaccaggc ggtcgatgcc
      421 gatgccctca cccgccgtcg gaggcatccc gtattcgagc gcgcgacaga agtcttcgtc
      481 gtagtccatc gcttcctgat caccgtgcgc tttgcttgcg acttgatcgg tgaagcggct
      541 gcgctggtcg tcgggatcgt ttagctcgga aaaggcgttg gcgaactctt tgccgatgca
      601 gaaaaattcg aagcggtcga caaagcgcgg atcgcggtcg tttttgcgcg aaagcggcga
      661 cacttccgtc ggaaattgac agacaaaggt cggctgcacg agcgactttt cgacaaactc
      721 ttcaaacagc gcgacgagcg catgtccgta gcctgtgggc gctttttcac cgagcaccga
      781 cttcgcatag cccggcagcg cagcgtattc cgcatcgcta caaggcccat cagcaatcgg
      841 cgcatcgggg cgcgcttctt tgacaagctg tcgcatcgtc gcccgacgga acggatggca
      901 caggttcaca cctttgtgtt cttgatagtc gatcgtcgtc gagccgttca ccgcttggca
      961 gacccgctcg atgagctgtt cggtcaaatc catcaggtct tcatacgtcg cgtaggcctg
     1021 atagaactcg atcgacgtga actccgggtt gtggaaacgc gacagacctt cgttgcggaa
     1081 aatgcggcca atttcataga cccgatcgat tccaccgacg acgagccgct tcagatccag
     1141 ctccgtcgca atccgcagat acaggtccag atcgagcgca ttgtgatggg tccggaacgg
     1201 tcgcgccgca gccccaccgt ttgtcgacaa gagcatccgg gtatcacact cgacgtagcc
     1261 gcgatcgtcg aagaagcgac gaatttcgcg cacaatcagc gcccgcttgc gaaacacgtc
     1321 ggcgacttcg ggatggacaa tcatgtcagc gtaacgctgc cgatatcgcc agtcttcgtc
     1381 gctgactttc ggtccgtctt gcagcgcttt gcccggaagt ggccgcaccg ctttgctcaa
     1441 aagacgcagt ttttcgaccc gcagcgcccg cttttctttg cgcgtcttga acagcggacc
     1501 cgacgacagc acaaaatcgc caagctgaag ctgattcgac agcgcaaacg attcggcctc
     1561 gtcggctttc aaatacagct gaagcatcgc gccccggtcg cctcggatga acaggaactt
     1621 ggccttgccc atctcgttga cttggagcaa acgaccggcg acgcagtaac gcggtgcatc
     1681 ggcggtcagc tcgttttcgt gcggcagctt ggcgacgtct tcgggttggc tcgccggcag
     1741 atcgaaaatc tggtgcgtcg gcgaaacatc gttggcaaat ggattgacac cttggctgcg
     1801 caggctcgcg atggcgcggc ggcgctcctc catctgtcct tcgaggtcac tgttcgatga
     1861 agcggcaggc gaagcagtct ggctcggcgc cgtcttggca gccggagagg taggcttttg
     1921 cgtcatgggg cgcctactct acctcatccg tgcgcaggcg cgaatagagc atcacgtcga
     1981 ggtgttgccc gtctttgaac gcctgtttgc gcagccgccc ctcgaagctg tatccgcact
     2041 tttccagcac gcgcgccgac gacacattcc aggcaaacac gcctgcttcg acgcgaaaca
     2101 gcgaaagctc atcgaatgcg tagcgcgtca ccgctttgac cgcttcggtc atcacaccgc
     2161 ggccccagta cggctctgcg agccagtaac caatctcggc ggtgtctcga tacaccggat
     2221 ctttgtcgag ccggtgcacc ccgattccgc ccaccgcgtg ctcgtcgatg acgatcgcaa
     2281 agacgtgctg gggcgcgggc aagctggccg cgtgcgccac ccagcgttcg gcatccttca
     2341 ccgtgtaggg gtgaggaaag ccgtcgcgca gattcagcca gatgtgtctg ttgttggcgt
     2401 gacgggccag cgactccacg tcacccgcaa caaacggacg gagtgtcagc ttggacaggt
     2461 gcaaaatcat gatgtagtca gtctagcgta accaaagttg tcgaaacgga agggaaactg
     2521 cccaatatga gcgattctgc ctcgcccgcg ccgtcaacgc cgccctctgc tgcatcgggg
     2581 aaaagcaaga tggcgcagac cgcgctgctt ctgtctttgg cgacgctgtt ttcgcgtgtg
     2641 ctgggacttg tccgcgagca ggtctttgcg gcgctgctcg gagcgggttt ttttggcgat
     2701 gcatttacga tcgcgttccg tttgccgaac ctgctgcgcg atctgtttgc cgaaggcgcg
     2761 ctgtcggctg cgtttcaacc ggcgtttcag gcggcgcgca aacaagaggg tcacgctgcg
     2821 gcgcagcggc tggcccagct tgtggcgacg tgtttgatcg tggtcgtcgg gctgatcacg
     2881 ctgctgggag tttggttttc ccccgaaatg gtcgggcact gggcgcaggg ttttgccagc
     2941 acccccggca aagccgagct gaccgtactg ctgacacgaa tcatgatgcc gtttcttttg
     3001 ctggtgtcgc tcgcggcgct ggcgatggga atgttaaatg ccgagcagcg ctttaccccg
     3061 ccggcgctgg ctcccgcgct gttcaacttg gcgacggtgg cgactggtct tggactgtgg
     3121 tttgtgggaa ttggccgatc caaaaccgca gcggttgcgt gggcggtggc gacgctgtgt
     3181 ggcggcggac ttcagcttgg acttcagctt gttccgcttt ggcgcagcgg ctaccgattc
     3241 cggctcgctt gggatttgtc tgatccgcgg ctcaaagcgg tgctgtgggc gatggcgcca
     3301 gcgacgattg gcctgatggc gacgcaagtg aacatttaca tcagctcgcg ctttgcatcg
     3361 acggaaccgg gggcggcaag ctggctgtat tacgcctttc ggctgctcca gatgccgatc
     3421 ggaatgttcg gtgttgccgt cggaactgtt gccctccagc gcgccgccga tgcggtcagt
     3481 gaagccgact tccgcgttgc aatggacgga gtgcgcgaca ccctgcaccg cggtctgcgt
     3541 ctggtgacgt tctattctct gcccactgcg gttgcattct ttgtgctcgc ggaacccatc
     3601 atcgccatca tttatcagcg cggcgccttc agcgcagaag acaccgtggc gacggcagaa
     3661 gcgctcaaat actactcgtt gggactgctt ttttattcgg cagtcaaagt cgtggtgccg
     3721 atcttttatt cgctcaagct cgcgcggttt gcggtgatgt cgaccgtgct gaccgtgacc
     3781 gggagcgtgc ttgtgaatct gtttttgcac ccccgctttg gcttccggat tttggcgttg
     3841 tcgacgagca taggcgcggc gataaacttt ctgtttctgg tcgtggtgtt tcgcgcccgc
     3901 tacggacagc tcttttcaca aacacttcag cttgggcttc tgcgcatgct ggtcgcgtcg
     3961 gtggcgatgg gcgcggtgct gtggattctg acgccgcttc tgctgggcga tgccgcacat
     4021 gcagccgcat attcccaagg tccgcagcat gtctcgctgt tgcgcgcgct tctgggcttt
     4081 tcggttctgt gcggaatcgg gggcggcagc tacgcttttc tgggctggct gttccgcctg
     4141 gaagaagtcg accacgtcct ctccgccgtc cgacgccgca tccgctagtc cgatcaggaa
     4201 tacgcgcgga cagacttccc tacagatcag ccgcagactc tgtttttgaa aaagtgacgt
     4261 tgcgttgcaa tcggatggca gagcgctgtc tctatgccaa cagaagttcc ggtgattcgg
     4321 aatggttccg atacaaacac ccactctgtt tccggcttag gagtcgacca tgcacggcat
     4381 atcacgacgc aacgcacttc gtacattcgg cggtttggga gccctcttcc tgttcggttg
     4441 cggagacagc ggcaccacag gcaacagcgc ggacggcagc caaagcggag acagcagcgg
     4501 tagttctggc agcgacggaa gcgttgttct ttcgggcggc ttcgcggtcg cgtctggagc
     4561 attcctaacc ggcaaagact acggcaatcc gttcgccagc ggaattggga caacctgcac
     4621 agcgtatgtc tcttccacca aaggaccgtg tcattccaat acctatctgc gcaaagacat
     4681 ttccgacgga cttgtcggtc tgcccacccg ttttgaactg atggttgtcg atgcgtcgtg
     4741 taagcccgtc ccaaacgcga tcgtcgaagt gtggtacgca tcgcccgcgg gaacgtactc
     4801 gaaagccgcg cagacgattg atgcgggcac cgggtacgga ggctctctgt cggatctgaa
     4861 tacgtcgttt tgtaccggca atgatgcgac tgcgcttgcg tcaaactggc tgcgcggcta
     4921 ccaaatcagc gacgccagcg gcagagtcac catcgacgga attttccctg gttggtattc
     4981 cggacgcacc agccacgttc acttcatcgt gacggccaat gggaagacct atgtgacctc
     5041 gcagcttttg tttgacgaaa cgctgacgac cgcggtctac accaaacatc agtcctacag
     5101 cgcgcgtggc aacaaagaca ccaccaacgc gaaagacaac gtggtgaaca tgggactccc
     5161 agtcgcatcg tccatcatgt cgttcagcca gcaaaatgac ggcgcactgg tctgcacaaa
     5221 agcgatcacc atcgcgtaag cccatccgtg gcggcctctc aaatcatctg aaagtcagat
     5281 tttcccgtca gactcggcac gcagcagagg ttgtagtaca ctacggtgcc atgatggatc
     5341 gggccacggg actggcaggt ttgggagtgc tgcttttggc gatctacccg ctctctgcaa
     5401 aaggactcga acccattttg ccttcgcggg tttgccaagc gctcgtcgat cagaaaccga
     5461 tcagcgagtg ttttgcggcc atcaaacagg ttccggtaga cccgctcgcc gctggggctt
     5521 gtctacggct gaaggtcaac tatccggttt cgatgtgcat gcgcaccatc gcgggcaagc
     5581 gctattcccc cgccgagatt tcggaatgtg acgacctgga agatgtctgg gaaaccatcg
     5641 ggtgctttta ccaaaacgga cagcgcgtgg ctccgcgcaa ctactcggga acgtatgcgg
     5701 atcaaatctg tccgaagctc cagagttcac gactgattcg gctctgtgtt gcggcgtttc
     5761 aggaatccta cattcagcct tccgcagcgc tcgcgtgtga ccgaatcaaa ggcgcaaagc
     5821 agacgctgga ctgtctgacc gcgatcatga accagcgcta cagcgaagcc gaagtggccg
     5881 cttgcgacgg gctcgcaacg agcaccgaaa ccatcgcctg cttttcgcgt gaagtcgcaa
     5941 gccccgacga agcagtcccg ccgccggtct attcacagtc cgacgcgccg ccgatgtatc
     6001 ctcgtccaca gcgttccgcg gaaccgggtg gctgcaacag ctatggctgt tatggtccag
     6061 ggggtggttg caacagttac ggctgttttg gatttggcgg tggctgtaac agctatggct
     6121 gctggaaaaa cggcggcggc tgcaacagct acggctgcta cggaatggcc ggtggctgca
     6181 atagctatgg ctgctggaaa aacggcggcg gctgcaacag ctatggttgc tggcattcac
     6241 gcta
//
LOCUS       DAORGN010000252         6117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6212_length_6117_cov_6.768228, whole genome shotgun sequence.
ACCESSION   DAORGN010000252 DAORGN010000000
VERSION     DAORGN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6212_length_6117_cov_6.768228"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PKL17_19440"
     CDS             complement(<1..1102)
                     /locus_tag="PKL17_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNK46968.1"
                     /translation="MSGLQGTPARPQHGDSFAALFEESLKHGDVKEGEIVRGRVIQIN
                     KDHVIVDIGDKSEGQVALAEFLGPDGQVMLKEGDEIDVYLESRENENGLCVLSKEKAD
                     RLKVWDEISAACERDELIEGVISQRVKGGLAVGIRGGVKAFLPGSQVDLRPVRNLDSF
                     IGKQFKFKVIKFNKKRGNIVLSRRVLLEKERAELKEQTLEKLTEGQIVEGIVKNLTEY
                     GAFIDLGGIDGLLHITDMSWGRVNHPSELFQVGDHVRVKVLKYNAETERVSLGLKQIT
                     DDPWSHAAERYTPGTVVKGKVVSLKDYGAFIELEEGIEGLVHISEMSWTRRVKHPSKV
                     VVVGDTVEAVVLDIDAKNNRISLGMKQLEPNPY"
     gene            1468..2406
                     /locus_tag="PKL17_19445"
     CDS             1468..2406
                     /locus_tag="PKL17_19445"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNK46969.1"
                     /translation="MESATTRRDGASDPPFPSELLPELIRSLPDGLLLFAAGDAPPLW
                     HNPQVGPLLGLSADELAGTTWRQLVQLLHDRSSTVEQPQSETRLPAELLPNATPIRGR
                     IAQHVESAGLMRLRVLDWSLRTIGSNNDRWLFMVIDVSPQHAIEQEGRRSETALQNVV
                     SEQQNLLYMLRQLGTPVLPIHEGIVVLPLIGHIDSARADHVMEAVLAAIVRYQAQVVL
                     IDITGVSLVDTAVANSLIQTVRAAHLLGTLSILVGISAEVARSMVQLGVQLNQMVTQR
                     DLQAGIGYALRHTGCAIVRRSQEVDWVATFAAPSQE"
     gene            2559..4886
                     /locus_tag="PKL17_19450"
     CDS             2559..4886
                     /locus_tag="PKL17_19450"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNK46970.1"
                     /translation="MTANPNADFEQSARSASSISGAIRVSQRAAGSGSRELEPRIADL
                     ARSPKIAVRDVISGSISALVTISYSLSFAAMIFSGPLKGHLEDGVRMSLMSAGLTLIL
                     VALTSPFFFAIAGPDSRSAAVQAAMAMMLTEKIADKDPSPHIVFALALSTAVTGVALY
                     VLGKLKMGRWVRYIPYPVVGGFLVSTGWVLAVGGMRVMVPPDVQVNFDLLPQLLQRGA
                     LAQTLFGVGFSITMFVVLGRAQQYFALPALLIAGTLLTHVIIAIDPDLNIAKAQESGW
                     LLRVSDNLNVSMPFIPFATGAMEWPAVRNFVFEVITLISVTAIAVLVTAAAIEVATKR
                     DADLDQELKGHGIANIVSGLCGGIVGSNAVARSMLNLQAGAATRLSGVIAGTLCLIVL
                     FVSPKLASLLSRPVLGATLVYLGLRLLQEWLFKAYWKLHRTDYMLVVLMLVLIIVTGI
                     FVVGLFVGALASCLIFAVNYSRVSVVKNSFTLDEYGSKVQRSAEENQVLRVRGRQYWV
                     LRLRGYLFFGSIVGLVADIRQRIRRSYAQDAQPLKSVVLDFRYVNGMDSSTALTLVKL
                     RQTIEDRRMQLIFTSLNAEMERALRREHCIRDDDVCVVFDDLDNALEWCEQRALVNEP
                     SLSRIDMPFVQRLAKEFGSLDLAVRFVDYVQRIELKKGEYLFRQGDASGATYVVESGR
                     VSIVLERPDGAPLLLRSVTSNTTLGEMGLYRQSIRSASVVADAPSVVHKLERSALDRM
                     ETEAPRVAAAFHTFVIRTLADRLHVADKAISALER"
     gene            5050..>6117
                     /locus_tag="PKL17_19455"
     CDS             5050..>6117
                     /locus_tag="PKL17_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46971.1"
                     /translation="MGASLLISCTTRQPLAPGCRPWTDSSSSTEPPAHCLFFPGSIAV
                     DPLGDVLYAANTNADLSFGGATVVAVDVLRHARAIECFRRYGSDPGTAGDSFCGSVSC
                     GDAGTVLGSAATVEETEAKEAATGQRPADFDRCYCQRDLDDSNVVNCESQRFVLSDQS
                     VKIGFFPGEMQLLAEDPPNWTALPDRAALRRSLLIAVRGDPSLTVIESARPLRRGRSG
                     SDSPDLRLSCDMGSRLSDADVAAPYSPHHAGDSYSLSTCGDNNRVNRTPDEIPVNPDD
                     PSLGTKPRLELPTEPMSVFVDRGCVEPGYRHDRGTFLSDKDQNPSGRPPCYKLDGANV
                     LPGHYYQYLVSSHLPTGQVSSF"
BASE COUNT         1117 a         1836 c         1841 g         1323 t
ORIGIN      
        1 catacggatt cggctcgagc tgcttcatac cgagcgagat gcggttgttc ttggcatcga
       61 tgtcgagcac gacggcttcg accgtgtcgc ccacaaccac gaccttcgac gggtgcttga
      121 cccggcgggt ccacgacatt tcgctgatgt gaaccagacc ctcgatgcct tcttcgagtt
      181 cgatgaaagc gccgtagtcc ttgagcgaaa cgaccttgcc cttgacgacg gtgccagggg
      241 tgtagcgttc cgccgcgtgg ctccacggat cgtcggtgat ctgcttgaga cccaggctga
      301 cccgctccgt ctccgcgttg tacttgagca ctttgacgcg gacgtgatcg ccgacttgga
      361 acagctcgga ggggtgattg acccggcccc agctcatgtc ggtgatgtga agcagaccgt
      421 cgattccacc gaggtcgatg aaggcgccgt actcggtcag gttcttgacg ataccttcga
      481 cgatctggcc ttcggtgagc ttctcgagcg tctgctcttt cagctcggcg cgctcctttt
      541 cgaggagaac gcggcgcgac agcacaatgt tgccgcgctt tttgttgaac ttgatgacct
      601 tgaacttgaa ctgcttgccg atgaacgagt ccaggttgcg aaccgggcga agatccactt
      661 gcgagcccgg aaggaacgct ttgactccgc cgcggatgcc gacggcgagg ccgcctttga
      721 cgcgctgcga aatcacgcct tcgatcagtt catcgcgctc gcaggccgcc gaaatctcgt
      781 cccatacctt gagacgatcg gctttctcct tcgagagaac gcagagtcca ttctcgtttt
      841 cgcggctctc caggtagacg tcgatctcgt ctccctcttt gagcatcact tggccgtctg
      901 ggccaaggaa ctctgcgagt gccacctggc cttctgactt gtctccgata tcaacaatga
      961 cgtggtcttt gttgatctgg atgactcgtc cacggacgat ctcgccttct tttacgtctc
     1021 cgtgtttgag gctctcctca aacaaggcgg cgaacgagtc gccatgctgt ggccgtgcgg
     1081 gggttccctg aagtccggac atgcgtttat tgaatctccc ttcgtaccgc tctcggctgc
     1141 cgccgacgag ggtgctctac gtaagcgcgc gtcaccgttc gcccaattcc aatacgctta
     1201 tgcgccactc gatgaaggcg aggcttttcg actgaggcag aagaactagt cgcgagggtg
     1261 atgaggaccg gggacagcac tgacgtagga gcgaaactta tgctgtcaca ttgtaggcag
     1321 tcaagagatt ttccatgaca aaaacacaaa aactgcaaag cctctatttg tgcgggttgt
     1381 gctcgcaggt agcacaggtc gtcatcgcgg gtagcgttca cggtcactgg ccaaaaacac
     1441 cagggtttcg tagcatcact ctttacgatg gagtcggcta ctacccgtag ggatggcgcg
     1501 tcagacccgc cctttcccag cgagcttctg ccggagctca ttcgatcact gccggatgga
     1561 cttttgctgt ttgcggcagg cgatgcacca cctttgtggc acaacccgca agtgggtccg
     1621 ctgctcgggc tgtctgccga cgagctggct ggcacgacct ggcgtcagct ggtccagctt
     1681 cttcacgata gaagcagcac ggtcgagcag ccgcaaagtg aaacccggct ccctgcggag
     1741 cttctgccca acgcaacgcc gattcgcggt cggatcgccc agcacgtcga aagcgctggg
     1801 cttatgcggc tacgggtgct cgattggagc ctgcgcacca tcggcagcaa caacgatcgc
     1861 tggctgttta tggtgatcga tgtgtcaccg cagcacgcga tcgaacaaga aggtcggcgc
     1921 agcgaaaccg cgctccaaaa cgtcgtcagt gaacagcaaa acctgctgta catgctgcgg
     1981 cagctgggca cgccggtttt gccgatccac gaagggatcg ttgtgctgcc gctgatcggt
     2041 cacatcgaca gtgcccgtgc cgatcatgtg atggaagccg tgctggcggc gatcgtccgt
     2101 taccaagcgc aggtggtgtt gattgacatc accggggtgt cgcttgtgga cactgccgtc
     2161 gccaattccc taattcagac cgtgcgtgcg gcgcatcttt tgggcacgtt gagcattttg
     2221 gtaggcattt ctgcggaagt tgcgcggtcg atggtgcagc tcggggtgca gctaaaccaa
     2281 atggtgacgc aacgtgacct tcaggcaggc atcggttatg cgctgcgcca caccggttgc
     2341 gcgattgtcc gtcgcagcca ggaagtcgac tgggtagcca cttttgcggc accgtcgcag
     2401 gaataatcgc ctacgctgcg gcgccaaaag ccgctgcgga cacatttgct gtagcgatac
     2461 cagcaatttg cgcaccgtgg caggccaaac cgtgtaggat gaaggtcatt ccgccgagcg
     2521 agagcgctcg ggggttgtgc ccttggtcga ggaacctgat gactgcgaac cccaacgcag
     2581 atttcgagca gagcgcgcgc agtgctagct cgatctcagg ggctatcagg gtctcgcagc
     2641 gagcagccgg ctcgggcagc cgcgagctgg agccacggat tgccgatctg gcgcgctcgc
     2701 ccaaaatcgc cgttcgcgat gtgatttcag gatcgatctc ggcgctcgtc accatcagct
     2761 acagcctttc gtttgcggcg atgatttttt cgggaccgct caaaggccat ctcgaagacg
     2821 gcgtgcggat gtcgctgatg agcgccggcc tgacgctgat tctcgtcgcg ctgaccagtc
     2881 cgttcttttt tgccatcgcg ggcccggatt cgcgatctgc ggcggtgcaa gcggcgatgg
     2941 cgatgatgct gaccgaaaag atcgccgaca aagacccgtc gccacacatc gtctttgcac
     3001 tggctttgtc gacggcggtt actggcgttg cactctacgt actcggcaag ctcaagatgg
     3061 ggcgctgggt ccgctacatc ccctatcccg tcgtcggagg cttcctggta tccaccggct
     3121 gggttcttgc cgtcggtggc atgcgcgtta tggtgccgcc cgacgttcag gtcaacttcg
     3181 atctgctgcc gcagctgctt cagcgcggag cactcgccca gacgctgttt ggtgtgggtt
     3241 tttcgatcac gatgtttgtg gtgctcggac gggcgcagca gtatttcgcg ctgccggcgc
     3301 tgctgatcgc aggcacgctg cttacgcacg tcatcattgc gatcgatccg gacctgaaca
     3361 tcgccaaggc gcaggaaagc ggctggcttt tgcgggtgtc ggacaacctc aatgtcagca
     3421 tgccgtttat tccatttgcg acgggagcga tggaatggcc ggcggtgcga aactttgtgt
     3481 ttgaagtcat cacgctgatc tcggtgacgg cgatcgcggt gctggtgacg gcggcggcga
     3541 tcgaagtcgc aaccaaacgc gacgccgacc tcgatcaaga gctcaaaggc cacggcatcg
     3601 ccaacatcgt atcgggcctg tgcggtggca tcgtcggtag caacgccgtc gcccgcagca
     3661 tgctcaatct tcaggccggt gcagcgacgc gactttcggg tgtcatcgcc ggaacgcttt
     3721 gccttatcgt gctgtttgtc agtccgaagc tggccagcct tttgtcgcgt ccggtgctcg
     3781 gcgcaacgct ggtgtatctg ggtctgcggc tcttacaaga gtggctgttt aaggcgtact
     3841 ggaagctgca ccgcaccgac tacatgctgg tcgtgctgat gctggtgctg atcattgtca
     3901 ccggcatctt cgtcgtcggt ttgtttgtcg gcgcgctcgc gagctgcctg attttcgccg
     3961 tcaattacag ccgcgtttcg gtggtgaaaa actcgttcac cctcgatgaa tacggcagca
     4021 aagtccagcg cagcgccgaa gaaaaccagg tcttgcgcgt gcgtggtcgc cagtattggg
     4081 tgctgcgact gcgcggctat ctattttttg gatcgatcgt tggtttggtc gccgacatcc
     4141 gccagcgcat ccgtcgctcg tatgcgcaag atgcccagcc gctcaagtcg gtggtgctcg
     4201 atttccgcta tgtaaacggt atggattcct cgacggcgct gacgctggta aagctgcgac
     4261 agaccatcga agacaggcgg atgcagctca tttttacgtc gctcaacgcc gaaatggaac
     4321 gggcgctgcg ccgcgaacac tgcatccgcg acgacgatgt gtgcgtagta ttcgacgatc
     4381 tcgacaatgc gcttgagtgg tgcgagcagc gagcgctcgt caacgaaccc agcctgtcgc
     4441 gaatcgatat gccgtttgtg caaaggctcg cgaaagagtt tggcagcctc gatctggcgg
     4501 tgcggtttgt ggactatgtg cagcgaatcg agctaaaaaa aggcgaatat ctgttccgtc
     4561 agggcgatgc gtctggcgca acttatgtcg tcgaatcggg ccgggtcagc atcgtgctcg
     4621 aacgacccga tggtgcgccg ctgctgttgc gatcggtcac gagcaacacc acgctcggtg
     4681 aaatgggtct ttatcgccag agcatccgat cggcgtcagt ggtcgcagat gcgccgtcgg
     4741 tggttcacaa gctcgagcga tcggcgctgg atcggatgga aaccgaagcc ccgcgcgtcg
     4801 ctgcggcctt tcacacgttc gtgattcgga cgctggcgga tcggctgcat gtcgccgaca
     4861 aagccatctc tgctctcgag cgatagtcga ttttccgacg ctcgttccag acggtcgacc
     4921 tggcgcgcgg cctgacccga tgcaagtact ttgattgacg cggcgttccg gctgtagcaa
     4981 ccttcgcaga ccgtgacagc gaaagcttgc gccttctacc cgcgattggg aagaaagctg
     5041 ctccttggtt tgggagcctc gcttctcatc agttgtacga cccgacagcc actggcccca
     5101 gggtgtcgtc cgtggaccga ctcatcgtcg agtaccgaac cccctgccca ctgcctgttt
     5161 ttccccggca gcatcgccgt ggacccgctt ggggacgtgc tctatgccgc caacaccaat
     5221 gccgatctta gcttcggtgg cgcaaccgtc gtcgccgtcg atgtgctaag gcacgcccgc
     5281 gcgatcgagt gtttccgtcg ctacggcagt gatccaggca ccgctggtga cagcttttgc
     5341 ggcagtgtgt cttgcggcga tgccggcacc gtcctcggct cggcggcaac cgtcgaagaa
     5401 accgaagcaa aagaagcggc gaccggccag cgcccagccg acttcgatcg ctgctattgc
     5461 cagcgcgatc tcgacgactc aaacgtcgta aactgcgagt cgcagcgctt tgtactctcc
     5521 gatcagtcgg tcaagattgg cttcttccct ggcgagatgc agcttctcgc ggaagatccg
     5581 ccaaactgga ccgccctgcc cgatcgagcg gcgctgcgtc gcagcctgct gattgcggtc
     5641 cgcggagacc cgtcgctgac cgtcattgag tccgcgcgcc cactgcgccg aggccggtcg
     5701 ggcagtgact cgcccgatct ccgtctgagc tgcgacatgg gcagccggct gtcggatgca
     5761 gatgttgcgg caccgtattc gccacaccac gccggcgata gctattcgct gtcgacgtgc
     5821 ggcgacaaca accgcgtcaa tcgcacgccc gacgaaatcc cggtaaaccc cgacgatcca
     5881 tccctaggca ccaagccgcg tctcgagctg ccgaccgaac cgatgagcgt tttcgtcgat
     5941 cgcggctgcg tcgaacccgg ctatcgccac gatcgcggca ctttcctgtc ggacaaagac
     6001 caaaatccga gtggccgacc gccttgctac aagctcgacg gggcgaacgt gcttcccggt
     6061 cactactacc aatatttggt atcgagccat ctgccgacag gccaggtgtc atcgttt
//
LOCUS       DAORGN010000253         5947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6513_length_5947_cov_6.695689, whole genome shotgun sequence.
ACCESSION   DAORGN010000253 DAORGN010000000
VERSION     DAORGN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6513_length_5947_cov_6.695689"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKL17_19460"
     CDS             <1..298
                     /locus_tag="PKL17_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46972.1"
                     /translation="KQSRLDGSGGEAGLFRRLSKSRAKPVVTLSAIDGLIKEAEAQVA
                     VLKTQKAELEADAKAEAAALAERKQKEAELVELRKLEAETKEKAKSLQNELGLH"
     gene            complement(323..940)
                     /locus_tag="PKL17_19465"
     CDS             complement(323..940)
                     /locus_tag="PKL17_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46973.1"
                     /translation="MILWAGCATSHRGRAEDTLRSYASAIARDDAGAAYQLLSPALSR
                     RLSPEEFSKQWRENRRELDDQRRQIEQQLAAAPRNLSEYAVVRLSQGSTLRLTAEKET
                     WGRVWRLGDANLQTVFAPTPQDALKLLLSAVEQRSYLALLRLLSEDERRALEAQISER
                     IERLRASLVKPTIEVRGDRARIEYDPRFFIELVREGNSWRIVDLN"
     gene            1260..1889
                     /locus_tag="PKL17_19470"
     CDS             1260..1889
                     /locus_tag="PKL17_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46974.1"
                     /translation="MPLVLGLLSALPGCDKDPAQAERRYCDQSGCYACVGENCYPVPG
                     DATKPPDAPTTSVSACDSDAACGAGRVCDIGKCVAACTDNSACKVGNNCVAGRCRPTD
                     AATCGLVGSLCTTDSACASGSTCVNRACATSCAVSGSKCALGQVCVSGACVEDPSPTS
                     PQCVFDADCGASGFRCINAYCLSNCTDNKQCQGGAVCQKGVCRGNRLPQ"
     gene            complement(1914..2627)
                     /locus_tag="PKL17_19475"
     CDS             complement(1914..2627)
                     /locus_tag="PKL17_19475"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK46975.1"
                     /translation="MSDAIKQNEQIYDRLWAKLPIPTHTEWPIWSELQKEIHPQARLL
                     EMGAGVLPRIPIEGGYFVDLSHAALRKLDENGGHGVRSAGPLPFADGSFDVVCAFEVL
                     EHIPDDDTVIRELARVLKPGGALFFSVPVDPKLWTGFDDACGHVRRYVASDLTARLSA
                     LGLAVERWTTQPNNFGKMSGDLAGMFLRAVAGLPKLTIWLKKRAVEGEMRLALRWRTD
                     DIDHSHADGGLIAIARKAA"
     gene            complement(2655..4226)
                     /gene="malQ"
                     /locus_tag="PKL17_19480"
     CDS             complement(2655..4226)
                     /gene="malQ"
                     /locus_tag="PKL17_19480"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNK46976.1"
                     /translation="MHGHPKLPGQSSWPRHSGLLLHITSLPGRGGCGDLGPSAYRFLD
                     QLLRGGQRSWQVLPIGPTGFGDSPYQTASVLGGNPLFVSIDKLVEDGLLPSDVLDAAP
                     AESATADFAAAQAYKWPLFERALQNLRAGQSEASMRLLREFAAYVEAERSWLDDYALF
                     MAIKEEQALRPWTDWPIPLRDRDPQALAAAQSRLSQRMDVHRFVEWQFERQWQALRHA
                     AHTRGIELFGDVPIFVAHDSVEVWSDRSQFLLHPDGRLQVQAGVPPDYFSKTGQLWGN
                     PLYDWKRMQADGFSFFRQRIRRAAALFDRVRIDHFRGFAAHWEIPAQDRTAEFGRWVS
                     APGHALFEVLTQDPDTKNVRFVAEDLGVITPDVEELRDRFAFPGIRVLQFGFGDDDFY
                     DGRPWAFRKNLVAYTSTHDNATLMGWYRGEPEGTRSDEQARRERDKLHDYLGHVPSPD
                     YAHFALIRLAMATPADTVIIPVQDVLGLGNVARMNTPGQPQGNWQFRLRSGQLDDRSL
                     DHLHHLTRLYARTSR"
     gene            complement(4270..5247)
                     /gene="trpS"
                     /locus_tag="PKL17_19485"
     CDS             complement(4270..5247)
                     /gene="trpS"
                     /locus_tag="PKL17_19485"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNK46977.1"
                     /translation="MFSGMQPSGGFHIGNYLGAVRDWVTLQDQHRCFYCIVDLHALTQ
                     PYAPEAMPGRVMDMAIELLACGIDPNRATLFVQSQVPEHAELAWVLSTVAPYGELSRM
                     TQFKEKGERAEYVNCGLFTYPVLMAADILLYQATRVPVGEDQIQHLELTREIVRRFNG
                     RFGEPPLFAEPLPLHRAPLRIMGLDGKTKMSKSLGNYVAISDEPDVIRKKIGGAFTDE
                     LRLRKKDPGHPESCYVCGLEPFFMPPDKVAEFHDGCRSARIGCVDSKRALAEAMITAL
                     APIQERKRELLAKPGLVNEILGDGAATARKVAAQTMVQVRERLGLLPLR"
     gene            5482..>5947
                     /locus_tag="PKL17_19490"
     CDS             5482..>5947
                     /locus_tag="PKL17_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46978.1"
                     /translation="MKRVLAALCALAGASGCYGTTYVETPAPQYGTTMEYIPEAPPQP
                     ISVTVAPPAPRYESVLTCGYGTIYVPGRWEWTGRWYWARGYCSPVRPGHIYVGPRFVG
                     GVYYRAHWAPSGYGAPAYPSGPMVSPAPVYRPAPVYGGPTVVAPPPPAPVYRP"
BASE COUNT         1148 a         1797 c         1917 g         1085 t
ORIGIN      
        1 aaagcaaagc cggctggatg gcagtggcgg cgaggccggg ctgttccggc ggctgtcgaa
       61 gtcgcgggcc aagccggtgg tgacgctttc tgcgatagac ggactgatca aggaagcgga
      121 agcgcaagtg gcggtcttaa agacgcagaa ggccgagctg gaagcagatg cgaaagcgga
      181 agcagcggcg ctggccgagc ggaaacaaaa agaagcagag cttgtggagc tgcggaagct
      241 ggaagccgag accaaagaaa aggccaagtc gctgcaaaac gagctgggcc tacactgacc
      301 gcccgccgtg cctgggtcgg ggttagttta ggtcgacaat ccgccagctg ttgccttcgc
      361 ggaccagctc gatgaaaaag cgcggatcgt attcaatccg cgcgcggtcg ccgcgaactt
      421 cgatggtggg cttgaccagc gacgcccgca gccgctcgat tcgttcactg atctgggctt
      481 cgagcgcccg tcgttcgtct tccgaaagca gccgcaagag cgccaagtag ctgcgctgct
      541 cgacggcgct gagcaacagc tttagcgcat cttgcggcgt cggggcaaaa acggtctgca
      601 agttcgcgtc gccaagccgc cacacccgac cccaggtctc tttttccgcc gtgagccgca
      661 gggtcgatcc ttgcgagagc cgcacaacgg cgtactcgga caggttgcgc ggcgcggcgg
      721 ccagctgctg ttcgatctgg cggcgctgat catccagctc gcggcggttt tctcgccact
      781 gtttcgaaaa ttcttccggc gaaagccgtc gactcagcgc gggggaaagc agctgatagg
      841 cggcaccggc gtcatcccgc gcaatcgcac tggcatagct gcgcagggtg tcttcggcgc
      901 ggccacgatg gcttgtcgca caaccggccc acaggatcac agcacacagg ctgcttgcgg
      961 agcctgggga tcgaaaaacg cgggtcgctg ggcgcgtgcg catgccgcat cgtacccgca
     1021 gcggcctacg ttcgcacacg cgaagcgttg ttcactgggt acacgttaac gtttgttcac
     1081 agaacccgcg gggtttgggc gtttctcgtg tggtcctcgg ctgacaaatc gatctagcaa
     1141 tgcggcacgg ggcttgtagg atggaactgt aaaagcttca aaagctctat cgctggagga
     1201 aaaccatgct ccgcactgcg atctcccgag ctggaaacag tcgtttgtca aacattgtga
     1261 tgccccttgt cctgggattg ctgtcggcac tgccaggatg cgacaaagat ccggcccaag
     1321 ccgagcgtcg ctactgcgat cagagcggct gctacgcttg cgtcggggaa aactgctatc
     1381 cggtccccgg agatgcgacc aagccgcccg atgcgccgac cacttcggtg tcggcgtgtg
     1441 atagcgatgc cgcgtgcggt gccgggcggg tctgcgacat tggcaagtgc gtcgcggcct
     1501 gtaccgacaa ctcggcgtgc aaagttggca acaactgcgt cgctggtcgc tgccgtccga
     1561 cggatgccgc gacgtgcggt ctggttggat cgctgtgcac gaccgattcg gcgtgtgcca
     1621 gcggatcgac ctgcgtaaac cgcgcctgcg cgacatcgtg cgccgtgtcg ggcagcaagt
     1681 gcgcgcttgg tcaggtctgt gtatcgggcg cttgtgtgga agacccgtcg ccgacttcgc
     1741 cgcagtgcgt ctttgacgcc gactgtggcg cgagtggctt ccgctgcatc aacgcctact
     1801 gcctgtcgaa ctgcaccgac aacaagcagt gccagggcgg cgcggtctgc caaaaaggcg
     1861 tctgccgcgg caaccgcctg ccccagtaaa tcccatcgcg cgagcgcgag cggttacgcc
     1921 gctttgcgcg cgatggcgat aagtccaccg tcggcgtggc tgtgatcgat gtcatcggtg
     1981 cgccagcgca gcgccagccg catctcgcct tcgaccgctc ttttcttcag ccaaatcgtc
     2041 agcttgggta ggccggcaac ggcacgcagg aacatcccgg ccagatcgcc gctcattttg
     2101 ccaaagttgt tgggctgggt ggtccagcgc tcgaccgcaa gaccgagcgc cgaaagccgt
     2161 gcggtcaaat cggacgcgac atagcgacgg acgtggccgc aggcgtcatc gaaaccggtc
     2221 cacagcttgg gatcgacagg gaccgaaaag aacagtgccc caccgggttt taacacccgc
     2281 gccagctccc gaatcacggt gtcgtcgtct ggaatgtgtt ccaggacttc aaatgcgcag
     2341 acgacatcga atgatccgtc ggcaaatggc aaagggcccg ccgaacgaac gccatggccg
     2401 ccgttttcat cgagcttgcg cagcgccgca tgggacagat cgacaaaata tccaccctcg
     2461 atgggaatgc gcggcagcac gccggcgccc atttcgagca gccgggcctg cgggtgaatt
     2521 tctttttgca gctcggacca aatcggccac tcggtgtggg tcgggatcgg gagcttggcc
     2581 cacagccgat cgtagatctg ctcgttttgt ttgatcgcgt cggacattgt tcgtttccgg
     2641 ggtagatggc gggactagcg ggacgtgcgg gcatacagcc gggtcagatg gtgcaggtga
     2701 tccaggcttc tgtcgtcgag ctgaccgctt cgcagacgga attgccagtt tccctgcggc
     2761 tggcccggcg tgttcatgcg cgcgacattg ccaaggccta acacgtcttg caccggaatg
     2821 atcacggtgt cagcaggtgt cgccatcgcc agacggatca gggcaaagtg ggcgtagtcg
     2881 ggacttggca catggcccag gtaatcgtgc agtttgtcgc gctcgcgccg ggcctgctca
     2941 tcgctgcgcg tgccttccgg ttcgccccgg taccagccca tgagcgtcgc gttgtcgtgc
     3001 gtcgacgtat acgcgacaag gtttttgcga aaagcccacg gacgaccatc gtaaaagtca
     3061 tcgtcaccaa agccaaactg gagcacgcgg atgccgggga acgcgaaacg atcgcgcagc
     3121 tcctcgacat cgggcgtgat gacgcccagg tcttcggcga caaagcggac gtttttcgtg
     3181 tccggatctt gcgtcagcac ttcaaacagc gcgtgtcccg gtgccgaaac ccagcgacca
     3241 aactcggcgg tgcggtcttg cgcggggatt tcccagtgtg ctgcgaagcc gcggaagtgg
     3301 tcgatgcgca cgcggtcaaa cagtgcggcg gcgcgacgga tgcgctgacg gaaaaaggaa
     3361 aatccgtcgg cttgcatgcg cttccagtcg tacagcggat tgccccacaa ctggccggtt
     3421 ttggaaaagt agtcgggcgg cacgccggcc tgaacctgaa gccggccatc ggggtgaagc
     3481 aaaaactggc tgcgatcgct ccacacttcg acgctgtcgt gggccacgaa gatgggcaca
     3541 tcgccaaaca gctcgatgcc gcgggtgtgg gcggcgtgac ggagcgcttg ccactgccgc
     3601 tcgaactgcc actcgacaaa gcggtggaca tccatgcgct gggaaaggcg gctctgtgcc
     3661 gcggcgagcg cttgcggatc acggtcccgc agcgggatgg gccaatcggt ccaaggccgc
     3721 agcgcctgct cttctttgat cgccataaac agcgcgtagt cgtcgagcca gcttcgttct
     3781 gcttccacgt aagcggcaaa ctcgcggagc agccgcatcg aagcttcgga ctgaccagcg
     3841 cggaggtttt gcagcgcgcg ttcaaacagc ggccacttgt acgcttgcgc agcggcaaag
     3901 tccgcggtcg ctgactcggc gggcgccgca tcgagtacat cgcttggcaa cagaccgtct
     3961 tcgaccagct tgtcgatgct gacaaacagc ggattgccac cgagcaccga cgcagtttga
     4021 tacggcgagt cgccaaaacc cgtcgggccg atcggcagca cctgccagga tctttgtccg
     4081 ccgcgcagaa gctggtcgag aaagcgatag gcgcttgggc ccaaatcgcc gcatccacca
     4141 cgtccgggca gcgatgtaat gtgaagaaga agtccgctgt gacggggcca gctggattgg
     4201 ccgggcagct tgggatgacc gtgcatgccg gcagtatatc gagatcaccg ccagcgccga
     4261 agcaaaccgc taccgcagcg gcaaaaggcc gagtcgctcg cgcacctgca ccatggtctg
     4321 cgccgcgact ttgcgggcgg ttgcggcccc atcgcccagg atttcgttga ccaggcccgg
     4381 ctttgcgagc agctcccgct tgcgttcctg aatcggggcg agcgccgtga tcatggcctc
     4441 ggcgagcgcg cgcttgctat cgacacagcc aatccgggcc gatcggcagc cgtcgtggaa
     4501 ttcggccact ttgtcgggcg gcataaaaaa cggttccagt ccgcagacgt agcagctttc
     4561 gggatgaccg ggatctttct ttcgcaggcg aagctcgtcg gtaaacgcgc cgcctatttt
     4621 tttgcggatc acatcgggtt cgtcagaaat cgcgacgtag ttgccaagcg atttcgacat
     4681 cttggttttt ccgtccagac ccatgatgcg cagcggcgcg cgatggagcg gcaggggttc
     4741 ggcaaacagc ggcggttccc caaatcgacc gttgaaacga cggacaattt cgcgcgtcag
     4801 ctccaggtgc tggatctgat cttcgccaac gggaacgcgc gtcgcttggt agagcagaat
     4861 gtccgcggcc atcagcaccg gatacgtaaa cagtccgcag ttgacgtatt cggcgcgctc
     4921 gcctttttcc ttaaactgcg tcatccgcga cagctcgcca tacggtgcaa ccgtgctcag
     4981 aacccaggcg agctcggcgt gttcggggac ttgggactgc acaaacagcg tcgcgcggtt
     5041 tggatcaatg ccgcaggcca gcagctcgat ggccatgtcc atcacccgac ccggcatggc
     5101 ttcgggggca tacggctgcg tgagcgcgtg cagatccacg atgcagtaaa agcagcgatg
     5161 ctggtcttgc agcgtcaccc agtcgcggac cgcgcccagg tagttgccga tgtgaaaacc
     5221 accggacggc tgcatccccg agaacactac ttcacggtga gaagtccgag ccggagcccc
     5281 ttgcgttgag tcagcgacgg aagaagaggc ggaagagtcg tgctttgggt ccatggcgca
     5341 gaagatgcat acagtgcgag tagcgtcgct gtcaaaatat cgtccttccg gacgaagcag
     5401 ctagcgttcg acgagagaaa gaaagttcgc ggatcgctcg tcggaaaaag taaaatggca
     5461 cagacacata ggagaaaagc catgaagcga gttcttgcgg cgctgtgtgc gctggccgga
     5521 gcaagcggct gctacggcac cacttacgta gaaacccctg ctccgcaata tgggacgacg
     5581 atggagtata ttcccgaggc cccaccgcag ccgatttcgg tcacggtggc gccaccggct
     5641 cctcgctatg aatcggtgct gacctgcggc tacggcacca tttacgttcc gggtcgctgg
     5701 gaatggacgg gacgctggta ctgggcgcgt ggatactgct cgccggttcg ccccggtcac
     5761 atctacgtgg ggcctcgctt cgtcggtggt gtgtactatc gggcgcactg ggcaccgtcg
     5821 ggatatggcg cgccagccta tccgtcggga ccgatggtat cgcctgcgcc cgtgtatcgg
     5881 cccgcgccag tatacggtgg accgacggtg gtagcgccgc cgccgcccgc gccggtgtac
     5941 aggcccg
//
LOCUS       DAORGN010000254         5943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6524_length_5943_cov_4.957371, whole genome shotgun sequence.
ACCESSION   DAORGN010000254 DAORGN010000000
VERSION     DAORGN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_5943_cov_4.957371"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..619)
                     /locus_tag="PKL17_19495"
     CDS             complement(20..619)
                     /locus_tag="PKL17_19495"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNK46979.1"
                     /translation="MGLFPPVPPEYAQLVARVDGFAADVMTRCAADFSCRAGCDDCCR
                     VELTLSLVEAAALAGSIAALAGEIKARLRRLLSSPIPTETPRCALLDESGQCAVYWAR
                     PLVCRSQGLPLRYPTDVVPVEAVRARLASGVVTVCPLNFAVREPDAGDVLDAERVDQI
                     LAVLNHRYAAAHGLDGQARYPLADVASAALAASASEPAS"
     gene            complement(623..2071)
                     /locus_tag="PKL17_19500"
     CDS             complement(623..2071)
                     /locus_tag="PKL17_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNK46980.1"
                     /translation="MHAHKRPGFGGRLVSTLLGVGLWSCSSGGFTPDPGKTDAGFDGG
                     GGDALVFDSVSPARGPSSGGIKVAIHGRRFDPGMRVSFAGVDSSLVSLTGEEEITATL
                     PPSGGKVGLVEVMLTRPDGKVAARSDLFSYYPGQLSFGSPRSFAVDNKPTKLVLGDLN
                     GDSKLDLIVASHSSGSLSLLNGKGDGNFDTYKRVSVGGTPAAMLTSEVTGDQFLDVIV
                     AGDNSGASLLLYPGKGDGTVGTVRSLATASAASLAGGDYNADGKYDIAVPYFAFAANG
                     IGFLFGKGDGTFTNGGSQGLRSGSFGISSADFNGDGKADLAVTMYDDSTVAILPSQGG
                     RFGSPSYLSAGAGPHGVIAADVNGDSKADIVVTDPDGGALRVFLGKGDSTFQPPTVIQ
                     TGGTTPQPVVAVDLNLDGWLDLVTVNVGSTGTVTILLGKGQGQFAAPQQLSVPGTHND
                     VVAADINGDRLPDLLLPVSNSASVSVLLNTSR"
     gene            2168..3394
                     /locus_tag="PKL17_19505"
     CDS             2168..3394
                     /locus_tag="PKL17_19505"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNK46981.1"
                     /translation="MALAMTSLAQPLATPPSDTPFLIRNEIRHNLAPHYPALRALRHR
                     RDLLQSPLYQQIEPALSPLLSAIECGEHQQPSPPRWSHGTLRVVAWNIQRGRMLPDIL
                     TALRDDPDLARADVILLSEVDHGMGRSHNRHVARELGQGLGMHYAFGVSYLVLGDDVM
                     ENAEGTENTLALAGSAVLSRWPIGALLNLDLPELRDKFSSKSEKRLGKKRALAAELHL
                     PSGPLWVSTCHLDSNASPMQRSVQLRSLLLEVMRHGQRILVGGDFNTTTYDASSKLAL
                     VRDLLHKFVKKGFDATVSGYMTPETSYEQPLFALLDELGFAHAGLNHRGQGSYYYDVE
                     SPFAEAKLIEKVGTWPTRWLQKRLRPWKGRVPAKLDWFVGKNLHAEAAGTVEVMGSDG
                     IPPSDHLPIFVDVSVP"
     gene            complement(3437..5500)
                     /locus_tag="PKL17_19510"
     CDS             complement(3437..5500)
                     /locus_tag="PKL17_19510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNK46982.1"
                     /translation="MRRLLFVAVPSLLLSFVGLAHAAPPAAETAVLPGEDCAKLAKRV
                     YGDGDAAVLHLKKNNPGVCDAPLAANAKLKTPPLAKKPAQNETPRLSFVGPAVRTKTK
                     GGWMEALPGQPVDKRMRIETSGQGGAEVTAGDKVKLQIPPNSKVVIKNLPKSGKAAEV
                     QLEQGSLRADMDDKAPTGPITVKTPGGDVKLQGDARIDADNNRAAVQVYEGQVAVRSK
                     GTTVNIKAGQGTVILRGTGAQALHDLPGAPAWQARDASEDGNRPFMVLAMAGLLEAQP
                     LGEVVVDFAQVPGAVRYIVEVARDPGFNDRRAGGEIAAPPLRVHLSPGKYYVRVSAVD
                     GDKFIGPPSAPRSFYIINVRTDGKLVAQGAPGPLTLTRSERVAIQLSGAGQPLLATLD
                     SGEPLDCSNEQKLALTDGQHKIHLQLGEAESELLVSVAPVTPPPPVLVGKLDGLTVPV
                     PLAVLGLPGRTLQPRTQVYALLGAGSTRPDASFTVGRLDVGGELALLQQRLSFDINVP
                     LMYQSNLVPPTDPTLASSGVAFGDVSLGARGVPLQALGGRLLLGPLLRLQLPTGSFPR
                     EATKGRPVVLDPAIGLAAILGRFGLQTTQGVTAALNLPQNQLRWAMSYMAEVQLWKLG
                     LLAQLDASLGLSGGTPAAASVGGGVRLHLDPFGILLGARGGIGSAGVDVYGRYYAFLG
                     FEWAP"
     gene            complement(5611..>5943)
                     /locus_tag="PKL17_19515"
     CDS             complement(5611..>5943)
                     /locus_tag="PKL17_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46983.1"
                     /translation="TDTGVTADMVYSAVAKTACGRCHGTGAGGLTFTDGATFRSSQVG
                     KKSGQATGLDMVKAGSVDGSYLIYKLMNQQTAPGVGGSGAIMPKGGTKLPDADLCKFI
                     VWVKEGAK"
BASE COUNT         1161 a         1946 c         1764 g         1072 t
ORIGIN      
        1 aaaacaataa atcgcctggc tacgacgccg gttccgaggc ggatgccgcc agcgccgcgg
       61 atgccacgtc ggccagtgga tagcgagctt gtccgtcgag cccatgcgcc gcagcatagc
      121 gatggtttag cacggccagg atctggtcca ctcgttccgc atcgagcaca tcgcccgcat
      181 cgggctcgcg caccgcaaaa ttcagcgggc agaccgtcac aacgccactg gccagccggg
      241 cccgcaccgc ttcgactggc acaacatccg tcggataccg cagcggcaga ccttgactgc
      301 gacagaccag gggccgcgcc caatagaccg cacactggcc cgattcatcg agcagtgcac
      361 accgcggcgt ttccgtcgga atgggcgatg acaaaagccg tcgcagccgc gcttttattt
      421 ccccagccag cgccgcgatc gaaccggcca gcgccgccgc ttcgacgagg gatagcgtca
      481 gttcgacccg gcagcagtcg tcacagccgg ctcgacacga aaaatccgcg gcacagcgcg
      541 tcatcacatc ggcagcaaag ccatcgacgc gcgccacaag ctgcgcatat tccggcggaa
      601 ccggtggaaa aagccccatg gcctagcgcg acgtattgag aagaacgctc accgacgcgc
      661 tgttcgacac cggcaacagc aaatcgggca ggcggtcgcc atttatgtcc gcagcgacga
      721 catcattgtg cgtccccggc accgaaagct gctgcggcgc cgcaaactga ccttggcctt
      781 tgcccagcag gatcgtcacc gttcccgtcg agcccacgtt gaccgtcacc agatcgagcc
      841 agccatccag gttcagatcc accgcgacca ccggctgcgg cgtcgttccc cccgtctgaa
      901 tcaccgtcgg tggttggaac gtactatcgc ctttgcccaa aaacacccgc agcgcaccac
      961 cgtcggggtc cgtgaccacg atatcggctt tgctgtcgcc atttacatcg gcagcgatga
     1021 caccgtgcgg tccggctcct gcgctcaggt acgatggcga gccaaaacgc ccgccttggc
     1081 ttggcaaaat cgcgaccgtg ctgtcgtcat acatcgtgac ggccaaatcg gctttgccgt
     1141 cgccattgaa gtccgcgctg ctgattccga agctgcccga gcgcagaccc tgcgaaccac
     1201 cgtttgtgaa tgtgccgtcg cctttgccaa acagaaagcc gatgccgttg gccgcaaacg
     1261 caaagtacgg caccgcaatg tcatatttcc cgtcggcgtt gtagtcgccc cccgccagcg
     1321 aggccgccga tgcagtcgcc aggctgcgga cagtgccgac ggtgccatca cctttgccgg
     1381 gatacagaag aagcgatgca ccgctgttgt ccccggccac aatcacgtcg aggaactggt
     1441 cgccggtgac ttcgctggtc aacatcgccg ctggagtacc tccgacggac acgcgcttgt
     1501 aggtatcaaa gttgccgtcg cctttgccgt ttaatagcga caggctgccg ctggaatggc
     1561 tggcgacgat caagtcgagc ttgctatcgc catttaagtc accaagcacc agcttggtcg
     1621 gcttgttgtc gacggcaaaa ctgcgcggcg aaccaaacga aagctgcccc ggataatacg
     1681 aaaacaggtc gctgcgcgca gccactttgc cgtcgggacg ggtcagcatg acttcgacca
     1741 ggcccacttt gccaccgctt ggcggcagcg tcgctgtgat ctcttcctcg ccagtcagac
     1801 tgaccagcga cgaatcgacc ccggcaaatg acacgcgcat ccccggatca aatcgcctgc
     1861 catggatcgc gactttgatg ccacccgacg acggaccgcg ggccggactc accgaatcga
     1921 agaccagcgc atcaccgccg cccccatcga acccagcgtc tgttttgcct ggatcgggcg
     1981 tgaaaccgcc cgagctgcag gaccaaagtc cgacgccaag cagcgtggaa accagtcgac
     2041 caccgaaccc aggacgtttg tgtgcgtgca tgtcggaaac aaaaaccgag ccacccgcac
     2101 agtcaacgaa aattcgagat cgattccgtg caggcgtcgg cttggcgctt ggtatatagg
     2161 ccaaaagatg gccttggcga tgacttcttt ggcgcaacca cttgcgaccc ctccgtcgga
     2221 cacaccgttt ttgatccgaa acgaaatccg ccacaatctg gcgccgcact acccggcgtt
     2281 gcgcgcactg cgccatcgtc gcgatttgtt acagagtccg ctttaccagc aaatcgagcc
     2341 tgcactgtct ccacttttgt cggcgattga gtgcggcgaa caccagcagc cgtcgcctcc
     2401 gcgctggtcg cacggcacgc tgcgggtcgt ggcgtggaac attcagcgcg gacggatgct
     2461 gcctgacatt ctgacggcgc tgcgcgatga cccggatctg gcgcgtgcag atgtgatttt
     2521 gctgtcggaa gtcgatcatg ggatggggcg ttcgcacaac cgccacgtcg ccagggagct
     2581 tggccaaggg ctcgggatgc actacgcatt cggggtgtcg tatttggtgc tgggcgacga
     2641 tgtgatggaa aacgccgagg gcaccgaaaa cacactggcg ctcgcgggat cggcggtgct
     2701 gtcgcgctgg ccaatcggag cgcttttgaa cctggacttg cccgagctgc gcgacaagtt
     2761 ttcatcgaag agtgaaaagc gactgggcaa aaaacgggcg ctcgccgcag agctacacct
     2821 gccgtcgggg ccgctgtggg tttcgacttg tcatctggac tcgaatgcgt cgccgatgca
     2881 gcgaagtgtg cagctgcggt cgctgctttt ggaagtgatg cggcatgggc agcgtatcct
     2941 cgtcggaggc gacttcaata cgacgacgta tgacgcatcg agcaagctgg cgcttgtgcg
     3001 cgaccttttg cacaagtttg tgaaaaaagg gttcgatgcg acggtttcgg gctacatgac
     3061 gccggaaacc agctatgagc agccgctttt tgcgcttctc gacgagctag gctttgccca
     3121 cgctggcctg aatcaccgcg gacaaggcag ttactactac gacgtcgaga gtccgtttgc
     3181 cgaagcgaag ctcatcgaaa aagtcgggac gtggccgacg cgctggctac aaaaacgact
     3241 gcgcccgtgg aaaggccggg tgccggcgaa gctcgactgg tttgtgggaa aaaatcttca
     3301 cgcggaagcc gcgggcaccg tcgaagtcat gggttcagat ggcattcccc cgtcggacca
     3361 tctgccgatt ttcgtcgatg tcagcgtgcc ctaatcacag cagtcgtcgc cgatggaacg
     3421 tgcgcgagac atgcggttac ggcgcccact caaagccgag gaaggcgtag tagcgaccat
     3481 agacatcgac gcccgcgctg ccgatgccgc cacgcgcccc gagcaggatg ccaaacgggt
     3541 ccaagtgcag ccgcacgccg ccgcccactg aagctgccgc cggtgtacca cccgacagac
     3601 ccagcgacgc atcaagctgc gccaaaagcc ccagcttcca tagctgcact tcggccatat
     3661 agctcatcgc ccagcgcagc tggttttgcg gcaggttgag cgcggcggtc acaccttggg
     3721 tggtttgcag cccaaagcgg cccaggatcg cagccaaacc aatcgccgga tcgagcacca
     3781 cgggacggcc tttggtcgct tcgcgtggga acgatcccgt cggtagctgg agccgcagaa
     3841 gcggtccaag cagaagtcgt ccacccagcg cttgcagcgg cacaccgcgc gccccaagcg
     3901 agacatcgcc aaacgccacg ccgctcgatg ccagtgtggg atcagtcggt ggcaccaagt
     3961 tcgattggta cataagcggc acattgatgt cgaacgacag tcgctgctgc aaaagtgcca
     4021 gctcgccgcc gacatcgagt cgcccgaccg tgaacgatgc atccggtcgc gtcgagcccg
     4081 caccgagcag cgcatacacc tgggtgcgcg gctggagcgt ccgtcccggc aaacccagca
     4141 ctgcaagcgg caccggaacc gtcagtccgt cgagcttgcc gaccagaacc ggcggtggag
     4201 gtgtcacagg cgccaccgac accagcagct cggactctgc ttcgccaagc tgaagatgaa
     4261 tcttgtgctg tccgtcggtc agtgccagct tttgctcgtt tgaacagtcg agcggctcac
     4321 ccgaatcaag cgtcgcgagc agcggctgac ccgcgcccga aagttgaatc gcgacccgct
     4381 cgctgcgcgt cagggtcagc ggccccggtg ccccctgagc gaccagcttg ccgtcggtgc
     4441 gcacattgat gatgtaaaac gatcgcggtg ccgacggcgg cccgatgaac ttgtccccat
     4501 ccacggcgct gactcgcacg tagtacttgc caggcgacag atggacgcgc agcggtggag
     4561 ccgcaatttc cccgccggct cgacggtcgt taaagcccgg atcgcgcgcc acttcgacga
     4621 tgtagcgaac cgcacccggc acttgcgcaa aatcgaccac cacttcaccg agcggctgcg
     4681 cttcgagcag acccgccatc gcgagcacca tgaacggtcg attgccgtcc tcgctcgcgt
     4741 ctcgcgcctg ccacgccggt gcccctggca ggtcatgcag cgcctgcgcg cccgtcccac
     4801 gcagaatgac ggtgccctgc ccggctttga tgttgaccgt cgtgcccttc gatcggaccg
     4861 cgacctggcc ttcgtacact tgcaccgcgg cgcgattgtt gtcggcgtca atgcgcgcgt
     4921 cgccttgcag tttcacgtca ccgcccggcg tcttgaccgt gatcggtccc gtcggtgctt
     4981 tgtcgtccat atcggcgcgc agcgaacctt gctctagctg cacttccgcc gccttgccgc
     5041 tcttgggcag gtttttgatc accactttcg agttcggcgg gatctgcagc ttgaccttgt
     5101 cgccggcggt cacttcagcg ccaccttggc cgctcgtctc gatgcgcatc cgcttgtcga
     5161 cgggctgacc gggcagtgct tccatccagc cgcccttggt tttggtgcgc accgccggac
     5221 cgacgaacga cagccgcggc gtttcgttct gggctggctt tttggcaagc ggtggcgtct
     5281 tcagcttcgc gtttgccgca agcggcgcat cacagacgcc agggttgttt tttttcaggt
     5341 gcaaaacggc ggcgtcaccg tctccgtaga cccgcttggc gagctttgcg cagtcttcac
     5401 cgggaagaac tgccgtctcc gcagcggggg gagcagcgtg ggcaagaccc acaaaagaaa
     5461 ggagcaggga aggaacggcg acgaagagca gcctgcgcat cgtcccgatt atatcgagaa
     5521 aaatggcagt ttaggacaca aatatctcaa gcaaagacgg cccgcggcca gcccacctgg
     5581 cgccgcggcg tggcgtccgc atcgagtcgg ttacttggcg ccttctttca cccacacgat
     5641 gaacttgcaa aggtcggcat cgggaagttt cgttccgcct ttgggcatga tcgcgccgct
     5701 gccgccgaca cctggagccg tctgctgatt catcagcttg taaatcagat agctaccgtc
     5761 gacgctgccc gctttgacca tgtccagccc ggtggcctgt cccgactttt tgccgacttg
     5821 gctggaacgg aacgtcgcgc cgtcggtaaa tgtcaaacca cccgcgcctg ttccgtgaca
     5881 gcgaccacaa gcggttttgg ccaccgccga ataaaccatg tccgcggtga cgccggtgtc
     5941 ggt
//
LOCUS       DAORGN010000255         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6554_length_5920_cov_5.569821, whole genome shotgun sequence.
ACCESSION   DAORGN010000255 DAORGN010000000
VERSION     DAORGN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6554_length_5920_cov_5.569821"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKL17_19520"
     CDS             <1..1113
                     /locus_tag="PKL17_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK46984.1"
                     /translation="KQDQVRQQHQKIFAQLNELLKLSEQFVRTSAAQARSERAVVQRT
                     MRRDTALLVGSSLLGMVTSLVVGFLVARRVARPIYELQLQVASAAQRTRIEVRPGRAG
                     LPALGEHVSAIVRRIEETDAEIVEQRRRLIQSEKMSAIGEVSAKLAHEILNPLAGIKA
                     AVQLLARGRPAEDGAASTVVRDTAQLMSQEVARVEQLVRRMLTYARPLQPRLSTCAVA
                     KLLSMAEEAARIEIEKAGVKVRRHEEAALPPVEVDQALVTQVLTNFLVNAAQASEPGS
                     DVVIESRRAQLDGKEAIELRVLDEGTGIAPAVLPNLFQPFVTTKPRGNGLGLAISQNI
                     AREHGGQISAHNRDGGKGAVFSLLLPLVHPLDEARV"
     gene            complement(1081..2430)
                     /gene="accC"
                     /locus_tag="PKL17_19525"
     CDS             complement(1081..2430)
                     /gene="accC"
                     /locus_tag="PKL17_19525"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNK46985.1"
                     /translation="MFKKILVANRGEIALRIIRACRELGIASVAIHSTADADAMHVKV
                     ADESVCIGPPPSRRSYLHIPAIISAAEITSADAVHPGYGFLSENAEFAEVCRRAGLTF
                     IGPLPEHMHLMGDKVQARTTMATHGVPVLPGTGIIKTDEEAFAAVDRIGLPVILKATA
                     GGGGRGMKIVHEKSRLRDQLHTARAEAQAGFGNPGVFIERFVDAPRHIEVQVIGDGKG
                     RVVALGERECSIQRRHQKLVEEAPSPALSPDERAQLLDRVAHAVAAVKYLSVGTLEFL
                     FERTPDGKPHFYFMEMNPRIQVEHTVTEMVTGVDLVREQIRIAAGIPLDVDGLREKSK
                     TRGHAIEVRINAEDPKTFAPSPGAITALHMPGGPGVRIDSHIYQGYVVPPHYDSLIAK
                     LIVHGEDRLSAIARLRRALDEFIIEGIKTNLDFHKRLTQMDDFVRAKLDTRFVERMH"
     gene            2583..3479
                     /locus_tag="PKL17_19530"
     CDS             2583..3479
                     /locus_tag="PKL17_19530"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNK46986.1"
                     /translation="MALLASDLVLPEFEGPLDLLLHLVRKHELDVFHIPISFITARYL
                     EMIERMRALNVDIAGEYLLMAATLAYLKSRELLPPDAKGEDEPPNPEEEGEDPRQALI
                     KRLLEYQRFKEAAQFLASRPVMGRNVFSRGVNPATLLGPEGEAPLAEIPVWELIELLA
                     KTLSKTRRKIAHEVTVERLSLADRINELCERLEQKSEHSFVGLLGELTKGETIDGLGY
                     QVVLTFMAVLELARLRLLHVSQDVDRGEILIRRVEGAILPLVPRQAELPFSENDQVAE
                     HTVPVENAVEQGPTSSDSEPNS"
     gene            3551..4765
                     /gene="scpB"
                     /locus_tag="PKL17_19535"
     CDS             3551..4765
                     /gene="scpB"
                     /locus_tag="PKL17_19535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNK46987.1"
                     /translation="MSSSEEGQDAGSEWADEDDVADHVAPPWGSMFGGPPLSIDGEGE
                     PSSEGQEVAWPLSGDRLQSVIESLLFAADKPLSVERIHELLEETAAAPHKPSGPPLYK
                     PQQIQSALEQLAQTLEQSGRGIGLLEVAGGWQLRTASTNAPWVQRLLKQKPVRLSRAQ
                     LESLAIVAYRQPITRPEIDDIRGVDSGGSLRTLLDRNLIRILGKKEEPGRPLLYGTTK
                     EFLEFFNLKDLKDLPTLREYYDLTEESRDKVRAAGFEPPTAVEQPGPSQGSLPVDGVL
                     APPSAEAAPVPAASATEPAVPTPDGATPAALSARPVELIDQQLADDAENLAKIDEMIR
                     AVNTAFPLLDPTLFGDPAAAAMVPGVQAKVVSEAKPPQVAAEAAADDASADDEPTDAE
                     PTGGPTERDDTE"
     gene            4762..5526
                     /locus_tag="PKL17_19540"
                     /pseudo
     CDS             4762..5526
                     /locus_tag="PKL17_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
BASE COUNT         1156 a         1746 c         1930 g         1088 t
ORIGIN      
        1 aaacaggatc aggtgcggca gcagcatcag aaaatctttg cgcagctaaa cgagctactc
       61 aaactgtcag agcagtttgt ccgcacgtcg gcggcgcagg cgcgaagcga acgggcggtg
      121 gtgcagcgga cgatgcggcg tgatacggcg ctgctcgtcg gcagcagtct gcttgggatg
      181 gtcacgagcc tggtcgttgg ctttctggtc gcgcggcgcg tcgcccgtcc gatttatgaa
      241 cttcagctgc aagtggcgtc ggcagcgcag cgaacccgga tcgaggtgcg accgggccgg
      301 gctgggcttc cagcgctggg tgaacacgtc agtgcgatcg tccgccgcat cgaagaaacc
      361 gacgcagaga tcgtcgagca gcgacgccgg cttattcaaa gcgaaaaaat gtcagccatc
      421 ggggaagtgt cggccaagct cgcgcacgaa atcctcaatc cgctggcggg catcaaagcg
      481 gcggtgcagc ttctggcgcg tgggcggcct gccgaggacg gcgctgcgtc gaccgtggta
      541 cgcgataccg cccaactgat gagccaagaa gtggcgcgcg tcgagcagct tgtccgacgg
      601 atgctgacgt atgctcggcc tcttcagcct cggctttcga cgtgtgcggt ggccaagctg
      661 ctttcgatgg ccgaagaagc ggcgcgcatc gaaatcgaaa aagccggggt caaggtgcgt
      721 cgtcacgaag aagcggcgct gccacctgtc gaagtcgatc aagcgcttgt cacccaagtg
      781 ctgaccaatt ttctggtcaa tgcggcgcaa gccagcgagc ccggcagcga cgttgtgatt
      841 gaatcacgac gggcccagct cgacggaaaa gaagcgatcg agctgcgggt cttggatgaa
      901 ggcactggaa tcgctccggc ggttctgccg aatctgtttc agccttttgt gaccaccaaa
      961 ccgcgcggca atgggctcgg gctggcgatc agccaaaaca ttgcgcgcga acacggcggt
     1021 caaatcagcg cccacaaccg cgacggggga aaaggcgcgg tgttttcgct tctgctgccg
     1081 ttagtgcatc cgctcgacga agcgcgtgtc tagtttggcg cgcacaaagt catccatctg
     1141 cgtcagccgc ttgtggaagt cgaggttggt cttgattccc tcgatgatga actcgtcaag
     1201 cgcccgtcgc agcctggcga tcgccgacag ccgatcttcg ccgtgcacga taagctttgc
     1261 gatgagcgag tcatagtgcg gcggcacgac gtagccctga taaatgtgcg agtcgatccg
     1321 cacgcccggt ccgcccggca tgtgcagcgc cgtgatcgct ccgggcgatg gcgcaaacgt
     1381 cttcgggtct tcggcgttga tgcggacttc gatggcatgg ccgcgcgttt tcgatttttc
     1441 ccgcagcccg tcgacatcca gcgggatgcc agcggcgatg cgaatctgtt cgcgaaccaa
     1501 gtcaacgccc gtgaccattt cggtgacggt gtgttcgact tggatgcgcg ggttcatttc
     1561 catgaaataa aagtgcggct tgccgtcggg agttcgctcg aacagaaact caagggtgcc
     1621 gaccgatagg tatttgacgg ctgcgacggc gtgggccact cgatcgagaa gctgcgcgcg
     1681 ttcgtcaggg gacagggcag gcgagggcgc ttcttcgacg agcttttggt gacggcgctg
     1741 gatcgaacat tcccgttcgc cgagcgccac gacgcggcct ttgccgtcgc caatgacctg
     1801 gacttcgatg tgacgcggcg cgtcaacgaa gcgctcgatg aagacgccgg ggttgccgaa
     1861 tccggcttgg gcttcggcgc gggccgtgtg gagctgatcg cgcagacggc tcttttcgtg
     1921 gacgattttc atgccgcggc caccaccacc tgcggtcgcc ttgaggatca ccggcagacc
     1981 gatgcgatcg acggcggcga aggcttcctc atcggttttg atgatgccgg tgccgggcaa
     2041 aaccggaacg ccgtgcgttg ccatcgtggt gcgggcttgg accttgtcgc ccatcaggtg
     2101 catgtgctcg ggcaggggac cgatgaacgt caggccggcg cgacgacaga cttcggcaaa
     2161 ctcggcgttt tccgacaaaa agccgtaacc gggatgcacc gcgtcagccg acgtgatttc
     2221 tgcggcgctg atgattgcgg gaatgtgcag atacgatcgc cgggatggcg gtggaccgat
     2281 gcagacgctt tcatcggcaa ctttgacatg catggcatcg gcgtcggcgg ttgaatgaat
     2341 cgccaccgaa gcaatgccca gctcgcgaca ggctcggatg atgcgcaggg caatctcgcc
     2401 acggtttgcg accaggatct tcttgaacat gggcccatag gacagccgaa gcttggggct
     2461 tttcgcaagt cgaaatcgtc cggctcgtct ctgggccggg cagcgtcgtt tttggctagg
     2521 cgcgttctcg atctgcggga ctggcaaact cgcgcgcggc tgctatagtc gcgccccttg
     2581 ccgtggcact gctcgcttct gacttagtcc tgcccgaatt tgaggggccg ctcgatttgc
     2641 tcctgcattt ggtgcgcaaa cacgagctgg acgtgtttca catcccgatc tcgtttatca
     2701 ccgcgcgcta cctcgaaatg atcgagcgga tgcgggcgct gaacgtcgac atcgcgggtg
     2761 aatacctgct gatggcggcg acgctggcgt acctaaaatc gcgcgagctt ttgccaccgg
     2821 atgccaaagg cgaagacgaa ccgccgaacc ccgaggaaga aggcgaagac ccccggcaag
     2881 cgctgatcaa gcggctgctc gaataccagc gcttcaaaga agcggcgcag tttttggcgt
     2941 ctcgtccggt gatgggtcgc aacgtgtttt cacgcggcgt caatcccgcg accctgcttg
     3001 gtcccgaagg tgaagcgccg ctcgccgaaa ttccggtctg ggaactgatc gaactgttgg
     3061 cgaaaacgct gtcgaagacc cgccgcaaga tcgcgcacga agtgacggtc gagcggcttt
     3121 cgctggccga tcgcatcaac gagctgtgtg aacggctcga gcaaaagtcc gagcattcgt
     3181 ttgtggggct gcttggcgag ctgaccaaag gcgaaaccat cgacgggctg ggctaccaag
     3241 tggtgctgac gtttatggcg gtgctggagc ttgcgcggct tcggcttttg cacgtaagcc
     3301 aagacgttga tcgcggcgag atcttgattc gccgagtgga aggcgcaatt ctgccgctgg
     3361 ttccgcgcca agccgagctg ccgttttcag aaaacgatca ggtcgcagag cacactgttc
     3421 ccgtcgaaaa cgcggtcgag caaggaccga cttcgtctga ttctgagcca aattcatgaa
     3481 acgcccgcat ccatcgcgcc ccgccgcgtc caccccactg cccgcgtccc cgtcggatga
     3541 agcgaccgct ttgtcatctt cggaagaggg ccaggatgcc ggaagtgaat gggcggacga
     3601 agacgatgtg gcggatcacg ttgctccacc gtggggcagc atgtttggcg gtccgccgct
     3661 ttcgatagac ggcgaaggtg aaccaagcag cgaaggccaa gaagtcgcgt ggccactgtc
     3721 tggcgatcgg cttcagtcgg tgatcgaaag tcttttgttt gccgccgaca agccgctttc
     3781 ggtcgagcgc atccacgaac tgctcgaaga gactgcggcg gcgccacaca agccgagcgg
     3841 accgccactt tacaaaccgc agcaaatcca atcggccctg gagcagcttg cgcagacgct
     3901 ggaacagagc gggcgtggca ttggactttt ggaagtcgcg ggtggttggc agcttcgtac
     3961 cgcgtcgaca aacgccccgt gggtgcagcg gctgctgaaa caaaaaccgg tgcggctgtc
     4021 gcgggcgcag ctggaatcgc tcgcgatcgt cgcctaccgt cagccgatca cgcgcccgga
     4081 aatcgatgac attcgcggtg tcgattcggg tggttcgctg cgcacgctgc tcgaccgcaa
     4141 cttgatccgc atcttgggca aaaaagagga accagggcga ccgcttttgt acggcaccac
     4201 gaaggagttc ctcgagttct tcaacctcaa ggacttgaag gatctaccga cgctgcgtga
     4261 gtattacgac ctgaccgaag agagccgcga caaggtgcgc gccgcagggt ttgagccgcc
     4321 gacggcggtg gaacagcccg gtccaagcca aggctcgctg cccgtcgatg gcgtgctggc
     4381 cccgccgagc gccgaagcgg ccccggttcc cgccgccagc gccaccgagc cagcggttcc
     4441 gacgccagat ggggcaacgc cggctgcact ttccgcaaga ccggtcgagc tgattgatca
     4501 gcagctcgcc gacgatgccg aaaacttggc caaaattgat gaaatgattc gggccgtaaa
     4561 cacggccttt ccgctgctcg acccgacgct gtttggcgat ccggcggcag cggccatggt
     4621 ccctggcgtc caagccaaag tggtttcaga agccaagccg ccgcaagtgg cggcagaggc
     4681 tgctgctgac gatgcgtcgg cagatgatga accgacggat gctgaaccga caggcggacc
     4741 gacagaaaga gacgacacgg aatgaaaggg attcgcttac aactgttgct ggccaaggct
     4801 ggcattgctt ctcgtcgcca tgccgagaca ctgatttccg aaggccacgt cgccgtaaat
     4861 gggcggatcg tgacgcagct ggggacgtgc gttgacccgg tcgccgaccg gatcagtgtc
     4921 gacggcaagc cggtcgaaca ggagccgctc gcttacttcc ttttgcacaa accaaaaggc
     4981 tacgtcacaa ccgcgtccga tccggacggg cgtccgacgg tgtttgacct gatgccggaa
     5041 gcgccggtgc ggctgtttcc ggtggggcgg ctcgactaca acacggaagg cgtgctgctt
     5101 ttgacaaacg acggcgagct ggcgcatgcg ctgatgcatc caagtcgtgg cgtcgaaaag
     5161 gtctatcacg ccaagttccg cgaggagctt acgtcggcga tgatttctcg tctgcagcaa
     5221 ggcgtggatc tgccgccggc gcgacctttg ggacgcgatg gtcagccgct gccggtatcg
     5281 aagccgatgg gaccgccgga gcgcagtgcg ccagcagaag tgcgggtgct gaagtttacg
     5341 tcgcgccata cctgggcgga aattcgactc cacgaaggaa aaaaccgtca gattcaccgc
     5401 atggcggaag cggttggatc gtcgctgctg aagctggttc gggtcgaata cgcgggactg
     5461 acggcagccg gggtcgagct gggcaatgcc cgtccgctgt cacgcggtga agtgtcgcgg
     5521 ctgcggctaa tgtgtggtct gtcggcgacg ctaccgcggg cggcccattc gaccgaggag
     5581 cgcgagccga gtcgtccgtt cgctcgacgt ccgtcgccgc gtggtttttc tgatcgccca
     5641 gatgaccgcc cgccgtcgcg ccgatatgaa gacaaacccg cgccgcgacg gtttgacgac
     5701 aggccgccgg ctcggaaata cgaagacaag cccactgcgc gacggtacga agacaagcct
     5761 gctgcgcgac gttcagatga tcggtcctcg cctcgtcaat tgggcgacag acccgcgccg
     5821 cgtcggtttg acgacaggcc gccggctcgg aaatacgaag acaagcccgc gccgcgacgg
     5881 tttgacgaca ggccgccggc tcggaaatac gaagacaagc
//
LOCUS       DAORGN010000256         5736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6889_length_5736_cov_4.649886, whole genome shotgun sequence.
ACCESSION   DAORGN010000256 DAORGN010000000
VERSION     DAORGN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6889_length_5736_cov_4.649886"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="thrC"
                     /locus_tag="PKL17_19545"
     CDS             complement(<1..818)
                     /gene="thrC"
                     /locus_tag="PKL17_19545"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNK46988.1"
                     /translation="MADLRAFFRCFSGCTGEWPLSLLSTRCPTCHGLLDVVHDLGPLR
                     ERSGASWRNLFDARRLDPTWPMPSGVWSKREWVHPDLCDEDIVSLGEGFTPLVPMPSL
                     GAALGVADLWIKQCGHNPTGSFKDLGMTGLVSQVQRLRRLGHRIPAVACASTGDTSAA
                     LAAYCARAGLPSLVFLPAGKLSDEQLTQPLCSHSLTVAIDADFDRCMELVAAFCAAHG
                     VYLANSMNPLRIEGQKTLSVEIVQQLGWQVPDWVVVPGGNLGHVTAIARGFAMA"
     gene            complement(828..2312)
                     /locus_tag="PKL17_19550"
     CDS             complement(828..2312)
                     /locus_tag="PKL17_19550"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HNK46989.1"
                     /translation="MQLLSSLRRALSLTGLGVGVLLAIVSVRSALTQSKQPKPTAPTV
                     AEFTSQEAAQRLGQAVRFATVSREAQPPAAAEFARMHEFLRESFPLVHSTLQRETVAE
                     LSLLYKWEGQDPTARPILLLAHQDVVPADVGADSGWEKPPFAGEVDGGFIWGRGTLDD
                     KCSMMAIFEAVEWLLSQGYRPSRTIYLAFGHDEEAVSSGGAQQVAELLKSRGIQLELT
                     LDEGLSVARGLMPGVQRPVALIGMGEKGFVNIEVEASGAGGHSSMPPKQTLVGKLARA
                     LTRLEEHQMPVELRPPATEMFEFVAPEMSWPLRPVLTNRWLFDPILRWQLGQSNSTRA
                     LLQTTQAPTMLSGSPKSNVLPQSVKATINYRIRPGDTSAAVLAHVRQVVADDSLRIDI
                     QPGSAVEPSPVSDSHAPIFATFAQTILSIFPDAVVAPSLMIGASDGRHFVRVSDSVYR
                     FVPFVIEPDDLTRFHGKNERIKVSDYLRMIRFYAQLVKHVDATR"
     gene            2401..4299
                     /locus_tag="PKL17_19555"
     CDS             2401..4299
                     /locus_tag="PKL17_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46990.1"
                     /translation="MTRQLGRRLSQGAPGGAWAPFVSGLLFAFLGCSQSAPNPGSVRF
                     LVRATPAPDDTVLSPLRDLRVTALELRDARTDDSLARSRFDPPASSVTGSPMLPALDL
                     GSLQVSESRDLRLLALGAAGQQLLGMALTRDVSWKFGESKDITLELRRPLFFFSGSPK
                     LVAPVPPTDPIFAPSRRIYEPLRDETKLRVIDPNSVTPLLSIYDRWIDPYNPSPGVTS
                     AQPVSAAAGTYDGQSLIVANLGGKVHIVDTLRLEDQGSFDLPEYMAQPAQSIVVDPLD
                     RTALVLQYAKAAVAVGRVGMIGFVRNLPSLRSRVSKDGELLMVSIDSTLSAPIGQPLA
                     AAYAPDGSVDVVFGKPPLSPGDPDCSTLGAGEPSTLRRYSPATGQQVSERSLPYTTSI
                     AYTLAGEQVLVQPCAAVSGATRLGRVVVGRAGGDKILSAPGVLAVSAIRSAVVGVGRD
                     DSADAPTLAAQARIFILESGADKWSSSSFDMPSWQVPYRITLSSDNKPYVSSIDISFA
                     PTDVNGYQLAVTPDRARALSLLRVQHKVRGLFLTYLGSSNQYRCYIDWSGYSYHVALL
                     NLQSGAREQDYIVGFQNQDCASRVYDSTNTYIGTCLPACDPADPKPYLRGFQDGYVPS
                     AASVLFGR"
     gene            4316..5251
                     /locus_tag="PKL17_19560"
     CDS             4316..5251
                     /locus_tag="PKL17_19560"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK46991.1"
                     /translation="MKKLLSCSLPLLLLSSPVSARTRTPKPPVQLAAAPASTSEPAQA
                     KAALPVDKLATDGVVVFEYRNDVKALSDLPDRLSQALVQNTSLRVLQLTEARRRLGAG
                     MDAEVARCDGETRCLSQVGQKLGVKEVLLLAVSQLGDVVLALQRIDVAEQRVTSRYAD
                     SLVSGQAIDEPRLLSWLQQLYPPDTFKRYGNIQIAANVSGAQVYVNSKASGMTPLAES
                     LHVLAPGNYRILVEKPKYLPFQAAITVMPDTTVEVSATLASELRQTPWYKRWYLWTGI
                     VLGAGAVAATAVAVKLATDVPPPDTSHLPGSIVFK"
     gene            5397..>5736
                     /locus_tag="PKL17_19565"
     CDS             5397..>5736
                     /locus_tag="PKL17_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNK46992.1"
                     /translation="MKTIGLIGAGNIGGTVAKLAVAAGYQVVLSNSRGPDSLASLVAE
                     LGPLARAATVSEAAAAGDLVVVTVPLRAYQSIPVEPLGGKVVIDTNNYYPARDGQFAE
                     LDSGAATVSGL"
BASE COUNT         1040 a         1713 c         1902 g         1081 t
ORIGIN      
        1 ttggccatcg caaagccgcg cgcaatcgcc gtcacatgtc cgaggttgcc gccggggacc
       61 accacccagt cgggcacctg ccagccaagc tgctggacga tttccaccga gagggtcttt
      121 tgtccctcaa tccgcagcgg gttcatcgag ttggccagat agacgccgtg cgccgcgcag
      181 aacgccgcca caagttccat gcagcggtca aaatcggcgt caatcgccac cgtcagtgag
      241 tggctgcaca gcggctgggt cagttgttcg tcggacagct tgcccgccgg cagaaacaca
      301 agcgacggca gcccggcccg cgcgcagtag gccgcaagcg ctgccgaggt atccccggtc
      361 gatgcacaag cgaccgccgg aattcggtgt ccaagccgcc ggagcctttg cacctgcgag
      421 acaagtccgg tcatccccag gtctttgaat gagcccgtcg gattgtgacc acactgcttg
      481 atccacagat cggccacccc cagtgcggct ccaagcgacg gcatcggcac aagcggcgta
      541 aagccttcgc caagtgaaac gatgtcttca tcgcacagat cgggatgtac ccactcgcgc
      601 ttgctccaca cgccgcttgg catgggccag gttgggtcaa gtcgccgggc gtcaaacagg
      661 ttccgccagc ttgcgccgct gcgctcacga agcggcccca gatcatgcac cacgtcgagc
      721 aggccatggc aggtcggaca gcgagtcgaa agcagcgaca gcggccactc accggtgcag
      781 ccggaaaaac agcgaaaaaa agcgcgcaag tccgccatcg ggattcctta ccgggtcgcg
      841 tcgacgtgtt ttacgagctg ggcgtagaag cgaatcatcc gcaggtaatc gctcaccttg
      901 atgcgttcgt ttttgccgtg gaagcgggtc aagtcgtccg gttcgatgac aaagggcaca
      961 aagcgataga cgctgtcgga cacccggaca aagtgtcgcc cgtccgacgc gccgatcatc
     1021 aggctgggcg cgacgacggc atcgggaaag atcgacaaga tggtctgggc aaaggtggcg
     1081 aaaatcggcg cgtgggaatc cgaaaccggc gatggctcga ctgctgatcc gggctggatg
     1141 tcgatgcgca ggctgtcatc ggcgacgact tggcggacat gcgcgagcac ggcggcggac
     1201 gtatcgccgg gccggatgcg gtagttgatg gtggccttga ccgactgcgg cagcacgttc
     1261 gatttgggac tgccggacag catcgtcggg gcctgcgtcg tttgcagaag cgctcgcgtc
     1321 gaattggact gcccaagctg ccagcgcaaa atcgggtcaa acagccagcg attggtcagc
     1381 accggcctca gcggccatga catctcgggc gcgacaaatt cgaacatttc ggtcgccggg
     1441 ggtcgcagct cgacaggcat ttggtgttct tcgagccggg tcagcgcccg cgccagcttg
     1501 cccaccagcg tctgtttggg cggcatcgac gagtgaccgc ccgcgccgga tgcttcgact
     1561 tcaatgttga cgaagccttt ttcacccatg ccgatgagcg cgacgggacg ctgcacgccc
     1621 ggcatcagcc cgcgcgccac cgacagacct tcatccagcg tcagctcgag ctgaatgccg
     1681 cgcgacttga gcagctcggc aacctgctgg gcaccgccgc tcgataccgc ttcttcgtcg
     1741 tgaccaaagg ccagataaat ggtgcggctg ggccgatagc cttggctgag cagccactcg
     1801 acggcttcaa aaatcgccat catcgagcat ttgtcatcga gcgtccctcg gccccagatg
     1861 aaaccgccgt cgacttcacc agcaaacggt ggcttttccc agcccgaatc agcccccaca
     1921 tcggcgggca caacatcttg gtgggcgagc agcaaaatgg gtcgcgcggt ggggtcttgt
     1981 ccttcccatt tgtacagcag cgacagctcc gcaacggttt cacgctgaag cgtcgagtgc
     2041 acaagcggaa agctttcgcg cagaaactca tgcatgcggg caaattccgc cgcggcgggt
     2101 ggctgggctt cgcgtgaaac ggtcgcaaag cgcacggctt ggccaagacg ctgggcggct
     2161 tcctggctgg tgaactcggc cacggtcggc gcagtcggtt tcggctgttt cgactgcgtg
     2221 agtgccgagc gaacactgac aatcgccaaa agaacgccca cacccaggcc agtgagcgag
     2281 agggctcgtc gcagagagga cagaagctgc atgttttggc caaagagtac accttgctgc
     2341 ccgcggcttc cacgagagaa agctgcgatg gtccgacacc gtttctgcta gagtccgacc
     2401 atgacgaggc agcttggtcg tcgattgtcg caaggcgctc ccggtggcgc atgggcgccc
     2461 tttgtgtccg gcctgctctt tgcgtttttg ggctgttcgc agtcggcgcc caatccgggt
     2521 tcggttcgtt ttttggtgcg ggccaccccg gcgccggacg atacggtgct atcgccgctg
     2581 cgcgacctgc gggtgacggc gctggagctt cgggatgcgc gcaccgacga ctcgctggcg
     2641 cgcagccgct ttgatccacc ggccagcagc gtgacgggtt ctccgatgct gccggcgctc
     2701 gacctgggtt cattgcaggt gtccgaatcg cgcgatctgc ggcttttggc cctgggcgcg
     2761 gcggggcagc agctcttggg aatggcgctg acccgcgacg tgtcgtggaa gtttggcgaa
     2821 agcaaagaca tcacgctgga gctgcgcagg ccgctgtttt tcttttccgg cagtccgaaa
     2881 ctggtggcgc cggttccacc caccgatccg atctttgcgc cgtcgcggcg gatttatgaa
     2941 ccgctgcgcg acgaaaccaa gctgcgcgtg attgatccga actcggtgac gccgcttttg
     3001 tcgatttatg accgctggat cgatccgtat aaccccagtc ccggcgtgac gagcgcccag
     3061 ccggtgagcg cagcggcggg aacgtatgac ggacagtcgc tgattgtcgc caatctgggc
     3121 ggcaaagtgc acatcgtcga tacgctgcga cttgaagacc agggcagctt tgacttgccg
     3181 gaatacatgg cgcagccggc gcagtccatc gtcgtcgatc cgctcgatcg caccgcgctt
     3241 gtcttgcagt acgccaaagc ggctgtggca gtgggtcggg tcggaatgat tggttttgtg
     3301 cgcaacctgc cgagtctgcg cagccgggtg agcaaagacg gcgagctgct gatggtgagc
     3361 attgactcga cgctgagtgc gccgattggt cagccgctgg ctgcggccta tgcgcccgat
     3421 ggaagtgtcg acgtggtgtt tggcaagccg ccgctttcgc cgggcgaccc ggactgctcg
     3481 acgctgggcg cgggggagcc gtcgacgctg cggcgctata gtcccgccac cggccagcaa
     3541 gtcagtgaac ggagcctgcc gtatacgacc agcatcgcct atacgctggc cggcgagcag
     3601 gtgctggtgc agccgtgcgc ggcggtaagt ggcgccacgc ggctgggacg ggtggtggtc
     3661 gggcgcgccg gcggcgacaa gatcctgtct gcaccgggtg tgctggcggt gagcgcgatt
     3721 cgttcggcgg tggtcggcgt cgggcgggat gattccgcgg atgctccgac gctggcggca
     3781 caggcgcgga tcttcatcct cgaaagtggc gcagacaagt ggtcatcgtc gagctttgac
     3841 atgccgtcgt ggcaagttcc gtatcggatc acgctgtcgt cggacaacaa gccgtatgtc
     3901 agctcgattg atatctcgtt tgcgccgacg gatgtaaatg gctatcagct tgcggtgacg
     3961 ccggaccggg cgcgggcact gtcgcttttg cgggtgcagc acaaggtccg cgggctgttt
     4021 ttgacctatc ttggatcgtc gaatcagtac cgctgctaca tcgactggtc ggggtattcc
     4081 tatcacgtcg cgctgctcaa cttgcagtcg ggcgcgcgcg agcaggacta catcgtcggc
     4141 ttccaaaacc aggactgtgc atcgcgagtg tacgacagca ccaatacgta catcgggacg
     4201 tgtctgccgg cgtgcgatcc tgccgatcca aaaccgtatc tgcgcggctt ccaagacggc
     4261 tatgtaccgt cggcagcaag cgtgctgttt ggacggtagc gaggagcctt gtgccatgaa
     4321 gaagctactt tcctgctcgc tgccgctttt gcttctgtcg tcgccggtat cggcgcgcac
     4381 gcggacgcca aaaccgccgg ttcagcttgc cgcggcgccc gcttcgacat cggaaccagc
     4441 ccaggccaaa gccgcactgc ccgtcgacaa gctggccacg gacggtgtgg tggtgttcga
     4501 gtatcgcaac gatgtaaaag cgctgtcgga tctgccggat cggctgtcgc aggcgcttgt
     4561 gcagaacacg tcactgcggg tgcttcagct taccgaagcg cggcgtcggc tgggcgcggg
     4621 gatggacgcg gaagtcgcgc gttgcgacgg tgaaacccgc tgtctgtcgc aggtcgggca
     4681 gaagcttggc gtcaaagaag tgctgctttt ggcagtgagc cagcttggcg acgtggtgtt
     4741 ggcgctccag cggattgatg tggcggaaca gcgggtcacg tcgcgttacg ccgattcgct
     4801 tgtgagtggg caggcgatag acgagccgcg tcttttgagc tggctccagc agctgtatcc
     4861 gcccgatacc ttcaagcgct acggcaacat tcagattgct gccaatgtca gcggtgccca
     4921 ggtgtacgta aactccaaag cgagtgggat gacgcctctt gcggagtcgc tgcatgtgct
     4981 ggcgccgggc aattaccgca tcttggtcga aaagccgaag tatctgccgt ttcaagcggc
     5041 gatcacggtg atgccggaca ccacggtgga agtatcagcg acgctggcga gcgagcttcg
     5101 gcagacgccg tggtacaaaa gatggtatct gtggaccggc attgtgctcg gcgcaggtgc
     5161 ggtggcggcg acggcagtgg cggtcaagtt ggccaccgat gtgcctccgc ctgacaccag
     5221 ccatctaccc ggcagcatcg tatttaaata acgcctgcgc cacgctgcgc gggccgtttt
     5281 ggaaagacaa ggggggcctt gcgttctggg cgcatgggcg cggggcgtgg gggctcgcaa
     5341 acggaatcgt tccacacgtt gcagcaaagg aaatccagat gagcacacag ggcgaagtga
     5401 agacgattgg tttgatcggg gccggcaaca tcggggggac ggtggccaag ctggcggtgg
     5461 cggcgggcta tcaagtggtg ctgagcaatt cgcgcgggcc cgattcgctt gcgtcgcttg
     5521 tcgccgagct gggtccgcta gcccgtgccg cgaccgtttc cgaagccgcg gcggcggggg
     5581 atttggtggt cgtgacggtg ccgctgcgcg cgtatcagag cattcccgtc gagccactcg
     5641 gcggcaaagt ggtgatagat accaacaatt actatccggc gcgcgatggg cagtttgcgg
     5701 aacttgattc tggcgcggcg acggtcagcg gtcttt
//
LOCUS       DAORGN010000257         5728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6906_length_5728_cov_5.533756, whole genome shotgun sequence.
ACCESSION   DAORGN010000257 DAORGN010000000
VERSION     DAORGN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6906_length_5728_cov_5.533756"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3856)
                     /locus_tag="PKL17_19570"
     CDS             complement(<1..3856)
                     /locus_tag="PKL17_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46993.1"
                     /translation="MAEPRVRRTETPLSTQDVEEYRALVKLSQSSALPTPDEAAQELT
                     RLCRLRNRPAILRCRLRFGRELLLVRVSDLDGDGKNEILVIDSDHNVHTPRFTVRQTE
                     SGPVASWTIGSHRYRGRLLGVSFSPFGVFHRQEAIVTSVEESDGSAVYRELKLTCDGQ
                     QWDVSELEHRSVSSMGLGEIRLIGDSSEVGRVPVLRGRPDDGRLWVEILDDRIGPKSP
                     LSTDFSKVSFLPGEVTDMVLTEDELVVAYARGGLQRISRDGRLRSVLFPQWRFVCLAY
                     APARTLTDRAVATGRGLLLGGTSFGYVFAMDLSQSHSGVQWRMDAGFLFTSMTLADVE
                     SSGRVDLVLGGIDGGMRIYELTEPQLLNQRCQKLYQSLGNKASDKLLELAPPRHEAHA
                     AVQLYLLSRVLESTGPATHAVVELVRWFTLGQPGWHTLPERAQREAVVSMEHFLLLHL
                     PQLATLEGRATPTGPLPLLTARQMQEGGPGELFAHGAFDPTTKLQLARTVLAELVEIY
                     LGAPRLVRHQIDRISRRLFEQYWQLAPALSSDSTAGEPNDQPPPELRKLLNVRSLRAR
                     EYSQTLAIESETRLQHLQSFPDGERPRLDENSDVVVRVLRAIGLTEELRTDRFSIELE
                     RLDLSATAIDPIDLTCDAPQMPTDVWPERCCVVGVGRHRSLRIEPLHPAHTTWDGKTP
                     ESEIMAWPWLPSRGGPELKLPGHVRFVEARRLWRDGDTILPTIIVGAEESGDTRLMVF
                     TPQRDGKLQLRGEVRGGGLGIEPIGFDCHLDETEGAPRDRSRLVESLVLRLPGTSERI
                     RPHVLVLDSQKGSPRLRSSLLPSELVAVVSTTGGSLYLTSARIQLQRPGEKQLWAPSP
                     AGVGCAALDETATTIAVGTSSGQVQVLHVEGESLRLHAQYLLPKGISALCFVPMGVFG
                     MPCLAIGTEDEQVHIVAIDYEHDGQEVTRLAAGGTPQRMLCRPMAEGGFRLLIVLSGG
                     VLTSWEAHPDDDHHESIQRLLDLGQRAAGSRVGLLRAALRGLGPHIRAVAMRELLRNA
                     REGRIAGSIFTTSEPKPLGTAGARPTQPGKTTPERGTPAVASVLHLCRLMLQGDAHST
                     GELPPRPTPGRSEVFDVAAWPLPRPPRHRLLVMQLFDSLVPIALGGAGSGADAQQSRL
                     SRELLGKLVQYCEEPLAVRGDLLQCIEQHVRADHIEELSMWLPPLILYYDTRFDTRPV
                     PWPLTVDGVAALFLRHLQRLDRAKLFDNAEPTFFRNIVAVLEAVGPTLPSRFLLTNLA
                     LLLRSALAWERRYLAGDLARRDF"
     gene            complement(3947..4390)
                     /locus_tag="PKL17_19575"
     CDS             complement(3947..4390)
                     /locus_tag="PKL17_19575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNK46994.1"
                     /translation="MQLSKLREQLLLQHASLRELVSEVAAQNARSQDEAGDEQLRRTL
                     GKLSAALSEHNRFEETSLRGIIVKLDAWGPQRESLMDSRHHDEHRLALEALEQSFATK
                     QRSERTERLAKLLDSILDHMKHEEREILHPNVLRDDVITADPFGG"
     gene            complement(4498..5565)
                     /locus_tag="PKL17_19580"
     CDS             complement(4498..5565)
                     /locus_tag="PKL17_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNK46995.1"
                     /translation="MQFETFTASTPAQSLSRAASAGHSGGVAVVLNRNARAVTRRMIR
                     RLHALHPRDHVFLSESFDHSRQIAEQLASSSYHTVLFGGGDGTFVCCLNDLREACRRK
                     GRPLPRVGVLPLGTGNAIGYYQGIRPSLRGVAGQLSQARITDGAVHNLPLLRVNNRLT
                     PFAGTGLDSQILEDYGATTRALDKMGLGRLIGSPLRYVLAVALRSVPRFVMRKLPEVE
                     VVNVGGPAYSIGLDGQPDKTPLPRGTVLYRGRCTLAGAATVPCYGFGVRIFPFADVRK
                     DMFHLRCTDASAGETLAHLPAVLRGDYRSPSLHDFLCDAVELRVSEPVPMQSGGDLIP
                     DRSDRMRIDLADTTVSLITPA"
BASE COUNT         1049 a         1818 c         1829 g         1032 t
ORIGIN      
        1 tgaaatcgcg ccgggccaga tcgcccgcca gatagcgtct ttcccacgcc agcgccgatc
       61 ggagcaaaag tgccaagttg gtcagcaaaa agcggctcgg cagcgtcgga cccactgctt
      121 cgagcaccgc gacgatgttg cgaaagaacg tcggctcggc attgtcgaaa agcttggccc
      181 gatccagcct ttgcaggtgg cgcaaaaaca gcgccgccac gccgtccacc gtcaggggcc
      241 acggtaccgg tcgggtgtca aagcgcgtgt cgtaatacag gatgagcggc ggcagccaca
      301 tcgacagctc ctcgatgtga tcggcgcgga catgctgctc gatgcactgg agcaggtcgc
      361 cacgcaccgc cagcggctct tcgcagtact gcaccagctt gccaagcagc tcccgcgata
      421 gacgcgactg ctgggcatcg gcaccgcttc ccgcgccacc gagtgcaatc ggcaccagcg
      481 agtcaaacag ctgcatcacc agcaagcgat gacggggcgg tcgcggcagc ggccaggcgg
      541 cgacatcgaa cacttcggac cggcctgggg ttgggcgcgg cggcagctct cccgtcgaat
      601 gcgcgtcgcc ttgcagcatc agccgacaca ggtgcaggac actcgcgacg gcgggtgtgc
      661 cgcgctcggg tgtcgttttt cctggctgcg tcggccttgc ccccgcggtg ccaagcggct
      721 tcggttccga cgtggtgaag atcgatcccg cgatccgacc ctcgcgggcg ttgcgcagaa
      781 gctctcgcat cgccaccgcg cgaatgtgtg gcccaagacc gcgcagcgcc gcgcgcaaca
      841 aaccgacgcg cgatcccgcg gcacgctggc ccagatcgag caggcgctgg atgctctcgt
      901 gatgatcgtc gtcgggatgg gcttcccaac tggtcagcac gccgccggac agcacgatga
      961 gcaggcgaaa tccaccctcg gccatcggac gacagagcat ccgctgcggg gtgccgccag
     1021 ccgcaagccg cgtgacttct tgcccgtcgt gttcgtagtc gatggcgacg atgtgaacct
     1081 gttcatcttc ggtgccaatc gccaagcacg gcatcccgaa cacgcccatc ggtacgaaac
     1141 acagcgcgga gatgcctttg ggcaggaggt attgggcgtg cagacgcagc gattctccct
     1201 cgacgtgtag gacttggact tgaccggagc ttgtgcccac cgcaatcgtc gtcgcagttt
     1261 cgtcgagcgc agcgcaaccg acaccggctg gcgacggtgc ccacagctgc ttttcacccg
     1321 gtctttgcaa ctggatgcgc gccgaagtca ggtacaaaga tcctcccgtc gtcgaaacca
     1381 cggcgaccag ctcactcggg agcagcgacg atcgcagacg cggactgccc ttttgggagt
     1441 cgagcaccag cacatgcggg cggattcgtt ccgacgtgcc gggcagccgc agcaccagcg
     1501 attcgaccag ccgtgatcgg tcgcgaggcg caccctcggt ttcgtcgaga tggcagtcaa
     1561 acccgatcgg ttcaatgccc aaaccgcccc cgcgcacttc gccgcgtagc tgcagtttgc
     1621 cgtcgcgctg cggcgtaaac accatcagtc gggtgtcgcc cgattcctcg gcaccgacga
     1681 tgatcgtcgg aagaatcgta tcgccgtctc gccacaggcg gcgtgcttcg acgaagcgga
     1741 catgtccggg cagtttcagc tcgggtccac cgcgtgacgg cagccacggc caggccatga
     1801 tctcgctttc gggcgtcttg ccgtcccagg ttgtgtgagc cggatgcagc ggttcgatgc
     1861 gcagtgagcg atgccgtccg acaccgacga cgcagcagcg ctctggccac acgtccgtcg
     1921 gcatttgcgg cgcgtcacag gtcaaatcga tcggatcaat cgccgtcgct gacaagtcga
     1981 gccgttcgag ctcaatcgaa aaacggtcgg tgcgcagctc ttccgtgaga ccgatcgccc
     2041 gcagcacccg caccaccacg tcgctgtttt cgtcgagacg tggccgttct ccgtcgggaa
     2101 aagactgcaa gtgctggagc cgcgtctctg attcgattgc cagcgtctgc gaatattccc
     2161 gcgcacgcag gctgcgcacg tttagcaact tgcgcagctc cggcggtggc tgatcgttcg
     2221 gttcgccggc tgtgctgtcg ctgcttagcg ccggggcaag ctgccaatac tgttcaaaca
     2281 gacggcgtga gatgcgatcg atctggtggc gcaccagacg cggcgccccc agataaattt
     2341 cgaccaattc cgccagcacc gtgcgcgcca gttggagctt cgtcgtcgga tcgaatgcgc
     2401 cgtgggcgaa gagttcgccg gggccgcctt cttgcatctg tcgggcggtc aaaagcggca
     2461 gcggtcccgt cggagttgcc cggccttcca gcgtcgcgag ctgcggcagg tgcaaaagca
     2521 gaaagtgctc catgctgacg acggcttcgc gctgggcccg ttcgggcagg gtatgccagc
     2581 ccggctgccc gagcgtaaac cagcgcacca gttccaccac cgcatgcgtc gctggcccgg
     2641 tgctttccag cactcgactt agcagataaa gctgcacggc ggcgtgtgct tcgtgacggg
     2701 gcggcgcaag ctccagcagc ttgtcgctcg ccttgttgcc gagcgactga tacagctttt
     2761 gacagcgctg gttgagaagc tgcggctccg tcagttcgta gatccgcatc ccgccgtcga
     2821 tcccgccgag gaccagatcg acgcggccac tcgattcgac atcggccagc gtcatcgacg
     2881 taaacagaaa tcctgcgtcc attcgccact ggacacccga gtgcgactgc gacagatcca
     2941 tcgcgaagac gtaaccaaac gacgtgccgc cgagcaaaag gccacgtccc gtcgccacgg
     3001 cgcgatcggt cagcgtgcgt gccggcgcat aggccagaca gacgaagcgc cactgcggaa
     3061 acagcaccga acgcagcctg ccgtcgcggc tgatgcgctg aagtccaccc cgcgcatagg
     3121 cgacgaccag ctcgtcttcg gtgagcacca tgtcggtgac ttcgccgggc aaaaacgaca
     3181 ccttgctaaa gtcggtgctt aaggggctct ttgggccgat gcgatcgtcg aggatttcga
     3241 cccacaagcg gccatcatcg gggcgaccgc gcaggacggg aacccggccc acttcgctgg
     3301 agtcgccgat gagtcgaatt tcgccaagcc ccatcgagct gacgctgcgg tgttccagtt
     3361 ccgatacatc ccactgctgt ccgtcgcagg taagctttag ctcgcggtag acggcgctgc
     3421 catcgctttc ttcgacggat gtgacgatgg cttcctgacg atgaaagacg ccgaacgggc
     3481 tgaaactgac gccaagcagt cgtccgcggt aacgatggct gccgatggtc cacgatgcga
     3541 cggggccgct ttcggtctgg cggacggtga agcgtggcgt gtggacgttg tgatcgctgt
     3601 cgatgacgag gatttcgttt ttgccgtccc cgtcgagatc cgagacacga accagcagca
     3661 gctcgcgacc aaagcgcagc cgacagcgca agatggccgg gcgattgcgc agccgacaca
     3721 gccgcgtaag ttcctgcgca gcctcgtcgg gggttggcag ggcactgctc tgcgacagct
     3781 tgacgagcgc gcgatattct tcgacatcct gcgtggacag cggcgtttcg gtacgacgga
     3841 cacgcggttc ggccatgtgc gcatcatacc tgcgcccgct ttggcttgca ggtgactcgg
     3901 tgtgcagaag cgatcgggga gacgacttct cgatgggaaa gggaagttaa ccgccaaatg
     3961 gatcggctgt gatcacgtca tcgcgcagca cgttgggatg caggatttcg cgctcctcgt
     4021 gcttcatgtg atcaaggatg ctgtcgagaa gcttggccag tcgctcggtg cgctcgcttc
     4081 tttgcttggt cgcaaacgac tgctccagcg cttcgagcgc caggcggtgt tcgtcgtgat
     4141 ggcggctgtc catcagcgat tcgcgctgcg ggccccacgc atccagcttg acgatgatgc
     4201 cgcgcaggct ggtttcttcg aagcggttgt gctcgctgag cgccgcggag agtttgccga
     4261 gtgtgcgtcg caactgttcg tcgccggctt cgtcttggct ccgcgcgttc tgtgccgcga
     4321 cctcgctgac aagttcgcgc agactggcgt gttgcaaaag aagctgctct cggagcttac
     4381 taagctgcat ggcggtctcc tctcgacgga agtaggccca tcctagcatg cagccaaggt
     4441 gagaatgccg gatatctcgt ctgacaattg cagaataagt cagcgacggg aaactgttta
     4501 cgcgggggtg ataagtgaca cggtggtgtc agccaggtcg atgcgcatgc ggtcgctgcg
     4561 atcgggaatc aggtccccgc cgctctgcat cggaaccggt tcggaaacgc gcagctcgac
     4621 ggcatcgcac agaaagtcgt gcagcgacgg actgcgataa tccccgcgca gcaccgctgg
     4681 caagtgcgcc agggtttccc cggcactcgc atcggtgcag cgcaggtgga acatgtcttt
     4741 gcggacatcg gcaaacggga aaatccgcac cccaaaaccg tagcaaggca ccgttgctgc
     4801 gccagcgagt gtgcagcgtc cccgatacag cacggtgccg cgtggcagcg gtgtcttgtc
     4861 tggctgtccg tcgagaccaa ttgaataggc cgggcctcca acgtttacga cttcgacttc
     4921 cggcagcttg cgcatcacaa agcgcggcac cgagcgcagc gcgaccgcca gcacgtagcg
     4981 cagcggcgaa ccaatcagtc ggcccaggcc cattttgtcg agcgcccgcg tcgtcgctcc
     5041 atagtcttcg aggatctgcg aatcgagccc ggttccggca aacggcgtca gtcgattgtt
     5101 gacgcgcaaa agcggcagat tgtgcaccgc gccgtccgtg atccgggctt gggaaagctg
     5161 accggcgacg ccgcgcagcg acggacggat tccctggtaa tagccaatcg cattgccagt
     5221 tcccagcggc agcacaccga cgcgcggcag cggtcggcct ttgcgtcgac acgcttcgcg
     5281 cagatcgttc aggcagcaga caaacgtgcc atcaccgccg ccgaacagca cggtgtgata
     5341 gctgctgctg gcaagctgct cggcgatttg acggctgtga tcgaagcttt cggacagaaa
     5401 gacgtggtcg cgcgggtgga gcgcgtgaag tcggcgaatc atccgtcgcg tcaccgcgcg
     5461 ggcgttgcga ttgagtacga cggcaacccc gccggagtgt ccagccgaag cggcgcggga
     5521 cagcgactgg gcgggtgtag atgcggtaaa cgtctcgaac tgcatgctgc gcggtacagc
     5581 atttcttggg ccagctgcta ggccgcgcgc agtctgcgat gtgtccgacg gagttttccg
     5641 tttgcccagg ctttgggctc tgcgcgacct ccgcgcgttg ccgcgttgcc tcggtgtcag
     5701 tgcatagccg tttacgcagt cgtggggg
//
LOCUS       DAORGN010000258         5718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6923_length_5718_cov_5.897934, whole genome shotgun sequence.
ACCESSION   DAORGN010000258 DAORGN010000000
VERSION     DAORGN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6923_length_5718_cov_5.897934"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKL17_19585"
     CDS             complement(<1..214)
                     /locus_tag="PKL17_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46996.1"
                     /translation="MAAFAIIVLIVAHRILRFDMLSDDFLMLRPQAVLLTLLALAVPL
                     ALWRRQLTLRFSTVLAVQWSLFLWALI"
     gene            complement(249..1409)
                     /locus_tag="PKL17_19590"
     CDS             complement(249..1409)
                     /locus_tag="PKL17_19590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK46997.1"
                     /translation="MSVRIITSEHELAALRVSWNQLAVGQPFRSWDWQVAWWRNYGRR
                     PGQRLHLVACFDGPQLIGLLPCYRQLTPQGRVLRPLASGEVCSEYLGILAAKDRAQEV
                     AEAIGDSLSGTGRQTDLSRTWELMLLDAQDASDEALLLLQAGLSRRGHPLETKPGVAC
                     WRLTVADDPTHPRGSDAYEECRFEGFLKQVARPHRNRLRRAERRGIANDYQFHMASDE
                     ASLAEGFSILVDLHQRRRAHHGQIGMFARPHFLSFHRDATQALLASGQLWLAWMTRQG
                     VPVSVFYCLADGPILHGYQSGLNPDYLDDEPGRILLLRLLRKALREGYLALDLLRGDE
                     PYKSHLGAERKYTQTVLIGNRTLVGKTAFGLALLRRKTRKLAGQVLRQLRAP"
     gene            1502..1915
                     /locus_tag="PKL17_19595"
     CDS             1502..1915
                     /locus_tag="PKL17_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46998.1"
                     /translation="MKNVSYLPNRTVPSRRRQPLSSLLRRGLSLIALVSLGGVLGCGP
                     GNLRATDLTPKERDVQLFENGEAPNCTLFEDYGTISVESGTSLSQGTYESSKAKLKRA
                     AAELGATSVRVTSQNTAGKIHKASGVAIKCLKREG"
     gene            complement(1928..3607)
                     /locus_tag="PKL17_19600"
     CDS             complement(1928..3607)
                     /locus_tag="PKL17_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK46999.1"
                     /translation="MFKLSEQEQQIVEALLAEPGSTTVEQLASALKLPVAGLLDLLGP
                     QQLLRAAALVEVPTSAELGHPHPTEPLFLARGLTTFAQRRESMPSQLPGLSILPTPEP
                     DASWAAEFVSDRPAQQVLELVKEKLVSTRPLLLFLSGCGAESTGLLAQAVRLRLSRPV
                     FFLDGGALSGWPLPDLTAALRRLRRDADLRGAALVVQDADLLGAAWRALTHPKPIGQT
                     APVLLCSGGTLVAPKFAPAASYGNPLQPHSFTLRVGSSPAAASGLATPAAGVADSEPS
                     EADRSREEARRLAALDAARAMGKPIPKELLSPAPAPATSSPSAQVAAPQSPAQATAAP
                     AQSARTAASPEPHQAAAATPPPPKVESAKLDAPQPAAQAPQAAARPTNPRLAAALAKA
                     GLLPPSRVEAAPAPAVSPAPAAAVLPAPAAVTTAPTPAAQVDPPVAAAASIATASESA
                     PTGSDDEADGPPLPLTDDAKLDDLVNIAKATTSMRQRAELLKRLAGTRSPAVIQLFRT
                     FSASPNTAVRLAAEAGMASLFGANWNRARTIAPPVQPPRTDDGGRGPGGAF"
     gene            complement(3793..4350)
                     /locus_tag="PKL17_19605"
     CDS             complement(3793..4350)
                     /locus_tag="PKL17_19605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNK47000.1"
                     /translation="MTTQQTQSSKTLAQAVAASRPALVRQARRMLRDETTAEDAAQDA
                     LVAALSHLDYFRGDSQLGTWMYRVGANAVLMTMRRERRASDRTTRAMTTLPKDSSWLY
                     GCGSSEPAQWKLEDSQQADLLRWAISQLPERYRLVVMHCDLDEKPVQEVAESLGLTVG
                     GVRTRRLRAHRMLRDTLRHRVDDLL"
     gene            4518..5594
                     /locus_tag="PKL17_19610"
     CDS             4518..5594
                     /locus_tag="PKL17_19610"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNK47001.1"
                     /translation="MTSINACTKLISVSKYRIQTVAQLTGLSSALIRAWEARYRLVVP
                     ERTDSGYRLYSDEDVAVLLGAQRLVKQGMAPMQIAKLPREQIVSGQRLQLVPGEVSAP
                     EPVVLRDPADEVATKDETNIRQPLSFAERIDQLLIAFANFDRPAADELLAAPLRHMPT
                     EQVCRELLLPLLVEVGERWHRGELTVAAEHFGTTLVRNRLMSLLDGLRRADRTHTVLC
                     ACPSGDLHEMGLLLFALEATIQGWEPVYLGPNVPLSDLLHSAQRVRPTLVALSFVMRH
                     DPSELRRLLSEAVTGLRGISPLLVGGSGLRGQSDVVRSTGCLMMPESGRLADLLPFLA
                     KDRTESPRVGKAPASGKTSKKFLR"
     gene            5644..5716
                     /locus_tag="PKL17_19615"
     tRNA            5644..5716
                     /locus_tag="PKL17_19615"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5677..5679,aa:Ala,seq:cgc)
BASE COUNT         1073 a         1695 c         1836 g         1114 t
ORIGIN      
        1 aaatcagcgc ccataaaaac agggaccact gaaccgccag caccgtcgaa aaccgcagcg
       61 tgagctgccg tcgccacagt gccagcggaa ccgccagcgc gagcagcgtc agcaagaccg
      121 cttgcgggcg gagcatcaga aaatcgtcgc tgagcatatc gaagcgcaaa atacgatgcg
      181 cgacgatcag cacaatgatc gcgaaggcgg ccacggtcgt ggaagttgaa cgtcgacggg
      241 tccgtctgtc aaggggcgcg aagctggcgc aggacctgtc cggccagttt gcgggttttt
      301 cgtcgcaaga gcgccagccc gaaagcggtt ttcccgacga gcgttcggtt gccaatcagc
      361 accgtctgcg tgtactttcg ttcggcgccg aggtggcttt tgtacggctc gtctcctcgc
      421 aagagatcga gtgccaagta gccttcgcgg agtgcctttc gcaaaagccg caacagcagt
      481 atccgccccg gttcgtcgtc gagatagtcg ggatttagcc cactttgata gccgtgcaga
      541 atcggcccgt cggccaggca gtaaaacacc gagacgggca cgccttgtcg ggtcatccac
      601 gccaaccaca gctgtccgct cgcgagcagc gcttgggtcg catcgcggtg gaacgacaaa
      661 aagtgcggcc tggcaaacat tccgatctgg ccgtggtggg cgcgtcgacg ctggtgcaaa
      721 tcgaccagga tcgaaaaacc ttccgcaagc gatgcttcgt cgctggccat gtggaactgg
      781 tagtcatttg caatgccgcg gcgttcggct cgacgcagtc ggttgcgatg gggccgtgcc
      841 acttgcttga ggaatccctc gaagcggcac tcttcgtagg catcgctgcc ccgtggatgg
      901 gttggatcgt ccgcgacggt cagacgccaa caggccacgc cgggcttggt ttccagcgga
      961 tgacctcgcc gcgaaagtcc ggcttgcaga agcaaaagcg cttcatcgct cgcgtcctgc
     1021 gcgtcgagca gcataagttc ccaggtccgc gagagatccg tttgtcgtcc cgtccccgag
     1081 agcgaatcgc cgatcgcttc tgcgacctct tgtgcgcgat cttttgccgc caaaatcccc
     1141 agatattccg agcagacttc gccactggcc agcggacgca gtacccgtcc ttgtggcgtc
     1201 agttgccgat agcatggcaa aagcccgatg agctgcggac cgtcgaaaca tgccaccagg
     1261 tggagccgtt ggccggggcg gcgaccgtag tttcgccacc acgccacttg ccagtcccaa
     1321 ctgcggaacg gctgcccgac ggcaagctga ttccatgaaa cccgcagcgc ggcaagctca
     1381 tgttcacttg tgatgattcg gaccgacatc gagtgcggca gtgtagcgct tgtttgccat
     1441 cagatgctgc tgcgcgtagg gcatagaaat cactgcgcct tgcgatgtag aatcagtgct
     1501 tatgaaaaac gtaagttatc ttcccaatcg aaccgtgcca agccgtcgtc gtcagccact
     1561 gtcgtcgctt ctgcggcgcg ggctgtcgct gatcgcactt gtgtcgcttg gtggcgtgct
     1621 cggttgtggt ccgggcaact tgcgagcgac ggaccttacc cccaaagaac gcgatgtgca
     1681 gctgttcgaa aacggcgaag cgccaaactg caccctgttc gaggactacg gtacgatctc
     1741 ggtcgagtcg ggcacgtcgc tgtcgcaggg aacctacgaa tcgagcaaag ccaagctcaa
     1801 gcgtgcagcg gcggagctgg gggcgacgtc ggtgcgagtc acgagtcaga acaccgccgg
     1861 aaagatccac aaagcgagcg gcgtggccat caagtgcctc aagcgtgagg gctaagccgc
     1921 agcgccgcta gaacgctccg ccgggcccgc gaccgccatc gtcggtacga ggtggctgca
     1981 ccgggggcgc gatggtccgg gctcgattcc agttggctcc aaacagcgac gccatgcctg
     2041 cttcggcggc cagcctgacc gcggtgttcg gactggcgct gaaggtgcgg aaaagctgga
     2101 tcaccgccgg gctgcgtgtt ccggccagtc gtttgagcag ctcggcgcgc tggcgcatgc
     2161 tcgtcgttgc tttggcgatg ttgaccagat cgtcgagctt tgcatcgtca gtgagtggca
     2221 gcggtgggcc atcggcttca tcatccgatc cggtcggtgc cgattcactg gctgtcgcga
     2281 tcgacgctgc cgctgcgacg ggcggatcca cctgtgccgc tggcgtgggc gcggtcgtca
     2341 cggctgcggg ggctggcaaa acagccgctg cgggggctgg cgaaacagcc ggtgccgggg
     2401 cagcttccac gcgactcggt gggagcaggc cggctttcgc aagcgctgcc gccaagcgcg
     2461 gattggtcgg tcgcgctgct gcttgaggcg cctgggctgc gggctgcggg gcatcgagct
     2521 tcgccgactc cactttgggt ggtggtggtg tcgcggctgc cgcttgatgg ggttcggggc
     2581 ttgctgcggt ccgtgcggac tgtgccggcg cagccgtcgc ctgagcgggg ctttgtgggg
     2641 cggccacctg ggcgctcgga ctggacgtgg ctggtgccgg tgcgggcgaa agcagctctt
     2701 tgggaatcgg cttgcccatc gcgcgtgccg catcgagagc agccagacgg cgtgcttcct
     2761 cacggctgcg atcggcttcg ctcggttccg aatccgccac gcctgctgcc ggggtcgcaa
     2821 gtccactggc tgcggctggc gagctgccga cgcgcagggt gaacgaatgt ggctgaagcg
     2881 gatttccata gcttgcggca ggggcaaact tcggcgcgac cagcgttcca ccgctgcaca
     2941 agagaaccgg tgcggtttgt ccgatgggtt tgggatgggt caatgctctc cacgcggcac
     3001 cgagcagatc ggcatcctga acgaccagcg ccgcgccacg tagatcggcg tcgcgacgca
     3061 gcctccgcag cgccgcggtt agatcgggca gcggccaacc actgagcgca cctccatcga
     3121 gaaaaaacac aggccgtgac agccgcagcc gcaccgcttg ggcgagcaga cccgtcgatt
     3181 ccgccccgca ccccgataga aacaacagca gcggtcgcgt gctgacgagc ttttctttga
     3241 ccagctccag aacttgctgg gctggacgat cggaaacgaa ctctgcggcc cagctcgcat
     3301 ccggttcggg cgtgggcaga atcgacagtc ctggcaactg cgacggcatg ctctcgcgcc
     3361 gctgcgcgaa cgtggtcagt ccgcgcgcca aaaacaaagg ttccgtcggg tgtggatggc
     3421 ccagctcggc gctcgtcggc acttcgacaa gtgcggcggc gcgcaacagc tgctgcggcc
     3481 cgagcagatc cagcagaccc gccaccggca gtttgagggc cgaggcaagc tgctcaaccg
     3541 ttgtactgcc tggctctgcg agcagcgctt cgacgatctg ctgttcttgt tctgaaagct
     3601 taaacaaacc atagaccgca cggagatcaa cagctggtgg acgggctgat ttgctgcggc
     3661 tggtcgtttt ttgggggcga ttcggagtgg ctgcgggggt cgtggccgag ggcgcaggct
     3721 ttttgctggg catagcctct cgaatatatc acgaggagag gccagtttgg acgaagcaag
     3781 cggcggctat gtctacagca aatcgtcgac gcggtgacga agcgtgtcac gcagcattcg
     3841 gtgcgcccgc agccggcggg tgcgaacgcc accgaccgtc agtcccagcg actcagcgac
     3901 ttcctgaacc ggtttttcgt cgagatcgca gtgcatcaca accaggcgat aacgctcggg
     3961 gagctgcgag atggcccagc gcaaaaggtc agcttgctga ctgtcttcca gcttccactg
     4021 cgcgggttcc gacgaaccac aaccatacag ccagctcgaa tctttcggca gcgtcgtcat
     4081 cgcacgggtt gtgcgatcac ttgcccgtcg ctcgcgccgc atcgtcatca gcacggcatt
     4141 ggcaccgacg cgatacatcc aggtaccaag ctgggaatcg ccgcggaaat agtccaaatg
     4201 ggacagcgca gcgacgagcg catcttgggc tgcgtcttcg gctgtcgttt catcgcgaag
     4261 catccgtcgg gcctggcgaa cgagagccgg tcggcttgcg gcgaccgctt gggcgagggt
     4321 cttgctggac tgagtctgtt gcgtcgtcat ggcaactctc cgatgtcttg aggaatgcac
     4381 agctggctta tagacaaacc ttatacaagc ctggcatgga tgtgtcaata agaaaaaatc
     4441 tatttttaga atctctctaa tttttcgtct gaaatttata aaatatcttg cgcaaatatt
     4501 cattcattga acaaaccttg acttcgatca atgcctgcac caagctgatc agcgtgagca
     4561 agtaccgaat ccagaccgtc gcccaactga ccggcctttc atcggcgctc attcgggctt
     4621 gggaagcacg ctatcggctc gtcgtgccgg aacgaacaga ctcggggtat aggctgtatt
     4681 ccgacgaaga tgtcgcggtt ttgctagggg cacagcgtct ggtcaagcag gggatggcgc
     4741 cgatgcagat tgcgaaactg ccgcgcgagc agattgtcag cggtcagcgt ttgcagctgg
     4801 ttcctggcga agtttcagcg ccagaaccag ttgtccttcg cgatccagcg gatgaagtag
     4861 caaccaaaga tgaaacaaac attagacaac cgctgagctt cgccgaacga atcgatcaac
     4921 tgttgattgc ctttgccaac ttcgaccgtc cagcagctga tgagcttttg gcggctcctc
     4981 ttcgccatat gccgacggag caggtctgtc gagagctgct actaccgctg ctcgtcgagg
     5041 tgggggagcg ctggcatcgc ggagagctga cggtcgctgc cgagcatttt ggtacgacgc
     5101 tggtgcgcaa tcggctgatg tcgctgctcg acgggctgcg ccgcgctgat cggactcaca
     5161 cggtgctttg tgcttgtccc tcgggcgatt tgcacgaaat ggggctgctt ttgtttgcgc
     5221 tggaggcgac cattcaaggc tgggagccgg tgtatttggg gcccaatgtg ccgctcagcg
     5281 acttgttaca ctcggcgcaa agagtacgtc cgacgctggt tgcgctgtcg tttgtgatgc
     5341 gtcacgaccc gagcgagctg cgccggttgc tgtcggaagc ggtgacgggg ctgcgtggta
     5401 tatcgccgct gctcgtcggt ggtagtggac tgcgtggaca atcggatgtc gtgcgttcaa
     5461 ccggctgtct catgatgccg gagagcgggc gtctcgcgga tctgctgccc ttccttgcga
     5521 aagaccgaac cgagtcaccg cgcgtcggca aggcgcccgc gagcggcaaa acatcaaaaa
     5581 agttcttgcg ttaggcgctc tctccttggt agaagttcgg aacctttcgg acggaaacgt
     5641 ctcggggctg tagctcagtt ggtagagcgc tagaatcgca ctctagaggc caggggttcg
     5701 actcccctca gctccaca
//
LOCUS       DAORGN010000259         5715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_6929_length_5715_cov_7.337102, whole genome shotgun sequence.
ACCESSION   DAORGN010000259 DAORGN010000000
VERSION     DAORGN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_5715_cov_7.337102"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="PKL17_19620"
     CDS             <1..1506
                     /locus_tag="PKL17_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47002.1"
                     /translation="SDETQILDRVSPAHMIQRIASSDPVPRAPAPTPGMTHRRPDPAP
                     MPPPVATPLRPQTPPRGLTPVAQTHSPAPPPIDADRPHGDVAGELSLADVLIADRDLL
                     ADPSGPPIPQPSRRAGDAHLPGVAREVRKSIPPAPAVKQAVAEEPPQSAKRAALLAAA
                     LEAPEPVRPTAAVRASAPATKAVKTVPAAVATPAPAVVPAPVVAAAPVPVPDSPRGIK
                     LPPIAKPGKTAAIPTIAPAPAAVTSSAGAPTLVGDVPVATDAPKPAPTVAAPVVENKS
                     AAPAKATATSKALAKEVKAAAVKSVAAEKPAAVVAPVAVEKPAEKVAAEKPAVEKPAL
                     EKVAAEKPVGDKPALGLPGLGKTASAAAKAPLPSKAESKPSPAPIAAKPAEVKAVENK
                     PAESKPAAVKVTDSKPVEAKAAESKPPAAKAAPVASKADPVTPVVSPAAAKAAAKPIA
                     QVKVTAAKAAAAKAAAEKAPVFDQSDLSDPSMSPSQFFRVFDAKTPFEDDK"
     gene            1635..3830
                     /locus_tag="PKL17_19625"
     CDS             1635..3830
                     /locus_tag="PKL17_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK47003.1"
                     /translation="MFGCRRKIVASVLGWLLLASGEVWADDDPFQTHVRAGREDGVSS
                     ERAHSAVSRSDIERRQPRSAPDALRYESGVFVQQSAHGQGSAFIRGLTGQQTLMMFDG
                     IRLNNSTYRQGPNQYFFTIDSRTVDTIEVLRGGGSTRYGSDALGGVIAAYPIDPLVLD
                     RELLFRPQLHFRTTSADDEVSGRAQVHVSGPIHGGDLGFVGGVGYRNVGALTGERVEN
                     PNPDTPLGRYPMVPAYEKDGITQRGTGFKELTADGRLVLRLGHTQRLTVATYHYLQFD
                     VPRTDQCPPPTAPPDTCLSYEQQFRHLGYLTYEGRLGDFLWPLRITVSVQAQHEKQRY
                     DDPSVLVVRTGIDDVLTLGFVANAKARPLVLRKDVQLRISYGFDQYTDWLTSTAFRTY
                     TDTLQTDQRSRGQYVSGSSYLYGGLYADASLALPKQVIVFGGARWSWIHATAPSDPAS
                     GTRAVNQTYLPVIGRVGTEWKPARLVALRFNIDHSFRAPNLNDLTARQQTGPGFQFEN
                     ADLRPERATTFELGALLEHSIVSAQLWGFETILNDAVLKVSKTLAECPPETPQCLGSW
                     SRLQLQNAPSYSELRGVEAMVRVRSDVGFGVRATLAYTFGETPRVGNIGYGAYGVIVG
                     ERVPMTRIPPLNGTAEAIYQRSSFEASLGVRWAAPQYRLALADYADARIPKYGTPGFV
                     VVDLRTSFRIHDRIVLGGVLENIGNAAYRYHGSSVNGGGRGFIMTLRID"
     gene            3891..5027
                     /locus_tag="PKL17_19630"
     CDS             3891..5027
                     /locus_tag="PKL17_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47004.1"
                     /translation="MKTMKNMICALGFVGILFGAACDSTTPMQQMPNLCVADTPATPM
                     CKPFTGALSSCKCSNDEYAPRFNNSKNDTWPACISDANPSKYVQLGSSLPASAARAQA
                     FVDMGTMLWKKGSTPTATDFTVARDKYSVAEGLGSRVARRQDIHYPEVPGDNKFACAD
                     QTVATMYPDRCASLAKLKPIVDAAFTAGIAGTKPLVQSARIEAALLWFFYLSPLSEEW
                     TSTFDDIEDVDSAWGYLTGGTDRGSPIGIGQYIQSLDGETYDRAFDGVLAGRCWRDID
                     KAFPSTCSLFYQRTTSQLDKALTRGMALILRDRIGKIPTLTGEAQEAALNFVNIIGGL
                     MDRAARAIDPAKADQLKTSTQATTAAGVNVTQAQSILDTLFACP"
     gene            5092..>5715
                     /locus_tag="PKL17_19635"
     CDS             5092..>5715
                     /locus_tag="PKL17_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNK47005.1"
                     /translation="MRKPTLLASLFLASIPSLSCVPGPDSVPPDSPPVIDPMKMPDPS
                     CRSGQTAHIGTNFFEDISDRSGIRVGNYVPSPTMPIPINDHSRLAFADLNGDGFDDIV
                     MHSLFPNPQKGVPFEHLVFLNNQDGTFRDFSDESGLRKVQAGFFAFGDIDNDGDQDAF
                     AGLDIPLGGQNHQILLNDGKGHFSPKANAGVEAPMGADTVTGNALFAD"
BASE COUNT         1149 a         1667 c         1752 g         1147 t
ORIGIN      
        1 agcgatgaga cacagatcct cgaccgggtg tcgccagcgc acatgattca gaggattgcg
       61 agctcggacc cggtgccgcg tgcacccgcg ccgacgccgg gaatgacgca ccgacggcca
      121 gatccagcgc cgatgccgcc acctgtcgcg acgccgctcc gtccgcagac gccaccgcgg
      181 gggcttacac ccgtggctca gacgcattcg cccgcgccgc ccccgatcga tgccgatcgt
      241 ccgcatgggg atgtggccgg ggagctgagc ctggcagatg tgttgattgc tgatcgcgac
      301 ttgcttgccg atccgtcggg gccgccgatt ccgcagccca gccgccgcgc tggcgatgcg
      361 catcttccgg gtgtcgcgcg tgaagtgcgc aagtcgattc cgccggcacc tgcggtcaaa
      421 caggccgtcg ctgaagaacc gcctcaatcc gccaagcggg cggcgctgct tgccgcggca
      481 ctcgaagcgc cagaaccggt caggcccacg gcagcggtca gggcttcggc cccagcgacc
      541 aaagcggtca agaccgtccc tgccgcggtg gcgacgcccg cgcctgctgt cgtcccagca
      601 cccgtcgtcg ctgcagcgcc ggttcccgtt cccgattccc cgcgcgggat caagctgccg
      661 ccgatcgcca aaccgggcaa aacggcggcg attccgacga ttgcgccggc tccggctgcc
      721 gtcacgtcat ccgcgggagc accgacgctg gttggggatg tgccggtggc gacggacgca
      781 ccgaaaccgg ctccgaccgt cgctgcgcca gtcgtcgaaa acaagagtgc ggctccggcc
      841 aaagcaacag cgacaagcaa agccttggca aaagaggtca aagcggcagc ggtaaagtcc
      901 gtcgcagccg aaaagccagc ggcggttgtg gctccggttg cggtggaaaa accggctgaa
      961 aaagtggcgg ctgaaaaacc ggcggtggaa aaaccggcgc tggaaaaagt ggcagcggaa
     1021 aagcccgtcg gggacaagcc ggcgctgggg cttccggggc ttggcaaaac cgcgagcgcg
     1081 gcagccaaag caccgctgcc gagcaaggcg gaaagcaagc cgagtcctgc gcccatcgcg
     1141 gccaagccgg ctgaagtcaa agccgtcgaa aacaaaccgg cagaaagcaa accggcggcg
     1201 gtcaaagtca ccgattcaaa gcctgtcgaa gccaaagccg cagagtccaa accaccggca
     1261 gccaaagccg cgcccgtggc gagcaaagcc gacccagtga cacctgttgt gtcacctgcg
     1321 gctgcaaagg ctgcggccaa gccgattgct caggtcaaag tgacggcggc gaaagcggcg
     1381 gctgccaaag cggctgcgga aaaagcgccc gtctttgatc agtccgacct ctccgatcca
     1441 tcgatgagcc cctcgcagtt cttccgcgtg ttcgacgcca agacgccgtt tgaagacgac
     1501 aagtagcacg cgctgttccg ctgacacgcg cctttccgcg ccccaaaacg cctttcgcta
     1561 ggggaatccc ctgtgcattt ggggtgatca ccggataggc gctttctgtt acgtctgtaa
     1621 aaatgtgccg gttcatgttt ggttgtcgtc ggaaaatcgt cgcttccgtg ctgggctggc
     1681 tgctgcttgc cagcggcgag gtgtgggctg acgacgatcc gttccagacg catgtccgtg
     1741 ccggtcgcga agacggtgtt tcgagcgagc gcgcccacag cgcggtcagt cgcagcgaca
     1801 tcgaaagacg gcagccgcgg tctgctcccg atgcgcttcg ctatgagagc ggcgtgtttg
     1861 tccagcagtc cgcgcacggt caaggatcgg cgttcattcg cggtctgacc gggcagcaga
     1921 cgctgatgat gttcgacggg atacgcctta acaactcgac gtaccggcag gggcccaatc
     1981 agtacttttt cacgatcgat tcgcgcacgg tggacacaat cgaagtgctg cgcggtggcg
     2041 gatcgacgcg ctatggatcg gatgcgctcg gcggagtgat cgcagcctat ccgattgatc
     2101 cgctggtctt ggatcgggaa cttctgtttc gaccgcagct tcactttcgc accacgtccg
     2161 cggacgatga agtgtctggt cgcgcgcagg ttcatgtctc tggaccgatc catgggggcg
     2221 accttggctt cgtcggtggc gttggttatc gcaatgtcgg tgcactcacg ggagagcggg
     2281 tcgaaaatcc caatccggac actccgcttg gccgctatcc gatggttcct gcgtatgaaa
     2341 aagatgggat cacccagcgc ggaaccggat ttaaggaact gaccgcagat ggccgtttgg
     2401 tgctgcggct gggtcatacg cagcgtctga cggtggcgac atatcactat ctacagtttg
     2461 atgttccgcg caccgatcag tgtccaccac cgacggcgcc tcctgatacg tgcttgagct
     2521 acgagcagca gtttcgccac cttgggtatc tgacctacga aggcagactt ggtgattttc
     2581 tgtggccgct gcgaatcacc gtgtctgtgc aggcgcagca tgaaaaacag cgctatgacg
     2641 atccatccgt gctggtggtt cggaccggga ttgatgatgt cttgacgctg ggttttgtcg
     2701 ccaacgcgaa agcacgtccg cttgtgctgc gaaaagatgt gcagctgcgg atctcctatg
     2761 gcttcgatca gtatacggac tggctcacct cgacggcatt tcgcacgtat acagatacct
     2821 tgcagaccga tcaaaggagc cgcggacagt acgtttcggg gtcttcctat ttgtatgggg
     2881 gactgtatgc cgatgcgtcc cttgcgcttc ccaaacaggt catcgtgttt ggaggggcgc
     2941 gctggtcgtg gattcatgcg acggcgccct ctgatccagc ttccggaacg cgcgcggtca
     3001 atcagacgta ccttccggtg attggcagag tcggaacaga atggaagccc gcacgacttg
     3061 tggcgctgcg gtttaacatc gaccattcct ttcgcgcgcc caacctaaat gacctgacgg
     3121 cgcgacaaca gacggggccc ggatttcagt ttgaaaatgc cgacctgcga ccagagcgag
     3181 cgacgacgtt cgagctgggc gcgctgctcg aacactccat cgtttcggcg cagctgtggg
     3241 gctttgaaac catcctaaac gatgccgtac tcaaggtatc aaagacgctc gcagagtgtc
     3301 ctccagagac gccgcagtgt ctgggatcat ggtcgcgctt gcagcttcag aatgcgccat
     3361 cctattcaga gctgcgtggt gtggaagcga tggtgcgtgt gcgcagcgat gttggttttg
     3421 gcgttcgcgc tacgcttgca tacacgtttg gagagactcc gcgggttggc aacatcgggt
     3481 acggagcata cggagtcatc gtcggtgaac gtgttccgat gacgcggatt ccaccgctca
     3541 atggaaccgc ggaagccatc tatcagcgga gctcgtttga agcatcgcta ggcgttcgct
     3601 gggccgcacc ccagtatcgc ttggcgcttg cggactatgc cgatgcgcga attcccaagt
     3661 atggaacacc tggttttgtc gtcgtcgatc tgcgcaccag cttccggatt catgatcgga
     3721 ttgtccttgg tggtgtgctt gaaaatatcg gcaacgctgc gtatcgctat cacgggtcaa
     3781 gtgtaaacgg aggcggacgc ggtttcatca tgaccctgcg aattgattga tcttttcccg
     3841 atggtcggga gtagcagtcg cttaatagac tggagaagga gaggatgcca atgaagacga
     3901 tgaagaacat gatttgtgct ttggggtttg tgggaatctt gtttggtgct gcgtgtgatt
     3961 cgacgacacc gatgcagcag atgccaaacc tctgcgtcgc ggacactccg gcgacaccga
     4021 tgtgcaagcc gttcacgggc gctctctctt cctgcaagtg ctcaaatgat gagtacgcac
     4081 cgcggtttaa taacagcaaa aatgacacct ggccggcgtg catttctgac gcaaatccga
     4141 gcaagtatgt ccagcttgga tcctctttgc cggcgtccgc tgcgcgtgcc caagcgtttg
     4201 tggacatggg aacgatgctg tggaaaaagg gaagtacacc gacagcaaca gacttcacgg
     4261 tggcgcgcga caagtattcg gtggccgagg gcctgggttc gcgagtcgcg cgtcgtcagg
     4321 acattcacta tccggaagtt ccgggtgaca acaagtttgc ttgcgcggat caaacggtcg
     4381 cgacgatgta cccggatcgc tgcgcttcgc ttgcaaagct gaagccgatc gttgatgcgg
     4441 cgttcacagc cggaatcgca ggaaccaagc cgcttgtgca gtcggctcgc atcgaagctg
     4501 cgctgctgtg gtttttctat ctgtcgcccc tttcggaaga gtggacttcg acgtttgatg
     4561 acatcgaaga tgtcgattcc gcgtggggtt acctaacggg tggaaccgac cgtgggagtc
     4621 caatcggaat cggtcagtac atccaatctc tcgacggtga aacctacgat cgggcgtttg
     4681 atggagtgct ggctggacgc tgctggcgcg acatcgacaa ggcgttcccc agcacatgca
     4741 gtctgtttta ccagcggacg acatcgcagc tagacaaagc gctgacgcgc ggaatggcgc
     4801 tgattctgcg cgatcgaatt ggcaagattc cgaccctgac aggagaagcc caggaagcgg
     4861 cgctcaactt cgtgaacatc atcggtggac tgatggatcg ggcggcgcgt gcgattgatc
     4921 cagccaaggc cgatcagctc aagacatcga cgcaggcaac cacggctgcc ggtgtgaacg
     4981 tgacgcaggc gcagtcgatt ctcgatacgc tgttcgcttg cccgtaacgc ccgcttcgct
     5041 tccatcgagc gtttccgttt gcaaaaaagc ggcctgtgat atggtccgct gatgcggaaa
     5101 ccgaccctcc ttgcctcgct ttttcttgct tctattccgt cgctcagctg tgttccgggg
     5161 cccgattcgg tgcctccgga cagtcctcct gtcatcgatc cgatgaagat gcctgatccc
     5221 agctgccgca gcggacagac tgcgcatatc ggaaccaatt tttttgaaga catttcggat
     5281 cgcagtggca tccgggtcgg caactacgtt ccttcgccaa cgatgccgat tcccatcaat
     5341 gatcacagcc ggctggcgtt cgcggatctg aatggcgatg gcttcgacga catcgtgatg
     5401 cacagcctgt ttcccaatcc gcaaaaaggg gttccgtttg agcacttggt cttcctaaac
     5461 aaccaagacg gtacgttccg tgacttttct gatgagtccg gactgcgcaa agtccaagcc
     5521 ggattttttg ctttcgggga catcgacaac gacggtgatc aggatgcttt cgccgggctc
     5581 gacattccgc ttggcggaca gaatcaccag attcttctaa acgatggcaa aggccacttt
     5641 tcacccaaag caaacgccgg ggtggaagcc ccgatgggag ccgatacggt gacaggaaac
     5701 gcgctgtttg ccgac
//
LOCUS       DAORGN010000260         5648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7039_length_5648_cov_5.334168, whole genome shotgun sequence.
ACCESSION   DAORGN010000260 DAORGN010000000
VERSION     DAORGN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_5648_cov_5.334168"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKL17_19640"
     CDS             complement(<1..504)
                     /locus_tag="PKL17_19640"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HNK47006.1"
                     /translation="MKIASIDCGTNSFHLMIGQISAQGQVEVVDRQKDMVRLGDSTFQ
                     NGVISPESFLRATEALRTFRKLCDSHQVDATVAVATSATREALNGGDFVRAVRDETGI
                     DLQVISGEEEARLIYLGARSVLPLGNRRALLFDIGGGSVEIMVCDARELYFQRSLKLG
                     VLRLLQMF"
     gene            complement(501..1532)
                     /locus_tag="PKL17_19645"
     CDS             complement(501..1532)
                     /locus_tag="PKL17_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47007.1"
                     /translation="MTRPSLGNLRDLELRKTRRLTAGQLHHQKPSFSPDGRFLLHALG
                     SGSESHWLLSDRKGRVARVLAGPALGRGAIAQDGSIAYGRQVGATCEIWLLAAGDSEP
                     KRLLGGDGRLYRDPTFSPDGSKLAFLADEGSQDSQLRLWIYDLAQGSRELLLGSLPGH
                     KLAQLAHPTWAPLGDSVFVEAILENGPTIFELVIGSGQIVARTDSGYAFPAPLARGLL
                     LAEHRPDPQHSELVLIAYPPCADKPKQVVLLDSKAGAREPAAVWDKKGEVHVAFTMTT
                     TQDGEPQRSDLHLAILSKLPRRLQKLGENDTDAASDEADSEGPKPKSARQADLPPLYD
                     IWLAMERGA"
     gene            1967..2596
                     /locus_tag="PKL17_19650"
     CDS             1967..2596
                     /locus_tag="PKL17_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47008.1"
                     /translation="MSASDGSSDTTSDRLKQRENGVVVSLGLTSRMPVVQVDGTTSAA
                     ANGIGGSLMAGYKLNRIIVGLGFSIDHFGQKTKLVAPSGTATSVTDTTSFLISPTVQV
                     GILRAAAGRLELYGSAQFGLGRAFLSRSNDPTLPSDVLATYDNKNFHLNYEVGPGLRF
                     YFIPQFALQLTSGVAGDHFFASQDNPSGRRTDSLSSVSLFGNLGLLGVF"
     gene            2641..3492
                     /locus_tag="PKL17_19655"
     CDS             2641..3492
                     /locus_tag="PKL17_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47009.1"
                     /translation="MRLLGIVGLLALSVGCDATPASTAPSTAREFLATYNQISCESRL
                     RCCGTVCSDASDDSFYKNYSRTLDYVAAGLYRFDPAAASACLASYSDFYKSCDTALTD
                     LPVVSAQCGAVIVGNTQAGGQCDSSLSPSNCVADTTCSGGTCMAKAKVGESCLTSSCV
                     AAAFCDTSVAMPVCKARLTAGQTCTASSQCQTTAFCDSSVLPRVCTDYGKEGQPCSAS
                     KLCDPTSGKLSCLPNQVCGGPQNDGAACASSTQCKSGRCAGVAPNQVCQAQPSPQTLR
                     DQLCGLK"
     gene            3668..5641
                     /locus_tag="PKL17_19660"
     CDS             3668..5641
                     /locus_tag="PKL17_19660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNK47010.1"
                     /translation="MSGKQQARFAPSGNIIVCGRAYNIGWPVVNFYDNPNFSAYRLGC
                     YKGKDGSPPGALPFGPAKGLGKSPYRYRERRLMGGQRNDLRRLQDIIRQFVLHHDGCA
                     TSADCFHVLHDERGLSAHFLVDNNGWIYQTLDLSDGAFHASGVNEISIGVELCNRGPV
                     ELDGPQFYSKRGISRDVTEVVVHNTRYRMWQYTQDQYLAMAALGKALARLLPNLPQVF
                     PEWNGQILNTWMADPRAFAGYIGHYHVTNNKWDPGCFDFKWLAQKIRVSSTWFVCLDK
                     NSECPKPPEIAENPQLAEQQGQVLFRNNEEDAMGGYFPVGPFGRSRLWHGGIHLNLPQ
                     GAPVFTPFPGRIVAARFGDDVPTGSANFVLMRHYFRLAGVETLFFILYMHVEREEPKS
                     GRTPWLFEADKKPFFSAMSSGGVFYPDIDLAGGEMIGHVGQAGPPGNYESQIHVEVMS
                     PEDLGSKLDAGYFSSIDGKSSGHFCDVRDIVSAIDRPKGKGDGILTEKELRDFFQNDQ
                     GKLELRKLAVRFQSEWGDANDWEQQLNLSKDFRAMPKPVRAKLYRDQIKPTLWYTAEL
                     AEKVGLPKDFVVWHYHPVRFIAWMNSQLRKQATTMVGQIQVAQGPAAAKVLDDRESLE
                     GFTDEEDELSPEASRRLTLEDLAGGYPDESPGK"
BASE COUNT         1047 a         1743 c         1722 g         1136 t
ORIGIN      
        1 aaacatctgg agcagccgca gcacaccgag cttcaggctg cgctgaaagt acagttcgcg
       61 ggcgtcgcag accatgattt cgacggagcc gccgccgatg tcgaacagaa gcgcgcgtcg
      121 attgccgagc ggtagcaccg atcgagcgcc caggtaaatc agccgcgcct cttcttcgcc
      181 gctgatgact tgcaggtcga tgccggtttc atcgcgcacc gcacgaacaa agtcaccgcc
      241 gtttaaggct tcgcgggtgg cgcttgtggc cacagcgacg gttgcatcga cttggtggct
      301 gtcacagagc ttgcggaacg tgcgcagggc ttcggtggcg cgcagaaacg actcggggga
      361 aatgacgccg ttttggaacg tggaatcgcc gagccgaacc atgtcctttt ggcgatcgac
      421 gacctcgacc tgaccttggg cgctgatctg cccaatcatc aggtggaacg aattggtgcc
      481 gcagtcgatg gatgcgattt tcatgcgccg cgctccatcg ccagccaaat gtcgtaaagc
      541 ggtggcaggt cggcctggcg tgccgatttg ggctttgggc cttcgctgtc tgcttcatca
      601 cttgccgcgt cggtgtcgtt ttcgccaagc ttttgtagcc gtcgcggcag cttcgacaaa
      661 atcgccagat gcagatcact gcgctgcggt tccccgtctt gcgtcgttgt catcgtgaac
      721 gcgacgtgga cttcgccttt tttgtcccag actgccgccg gttcccgcgc gccggctttg
      781 ctatcgagca gcaccacctg tttgggctta tcggcacacg gcggataggc gatgagcaca
      841 agctcgctgt gctgcggatc gggccggtgt tccgccaaca gcaagccgcg cgcaagcggg
      901 gcagggaacg catagcccga atcggtgcgg gcgacgattt ggccgctgcc aatcaccagt
      961 tcaaaaatcg ttgggccgtt ttccaagatc gcttcgacaa agacgctgtc gcccagcggc
     1021 gcccacgtag gatgcgcaag ctgcgccagt ttgtgtcctg gcagactgcc gagcagaagc
     1081 tctcggctgc cttgcgccag atcataaatc cacagccgaa gctgcgagtc ttgactgcct
     1141 tcatcggcga gaaaggccag cttcgacccg tcgggggaaa acgtgggatc gcgatacagc
     1201 cggccatcgc caccgagcag ccgcttgggt tcgctgtcgc cagccgccag cagccagatt
     1261 tcacaagtgg cgccgacttg ccggccatag gcgatgctgc cgtcttgtgc gatggcgcct
     1321 cgtccgagcg cgggtccggc cagaacgcgc gccacccgtc ctttgcgatc ggacaaaagc
     1381 cagtgcgatt cgctgccgct gcccagcgca tgcagcaaaa accgcccgtc gggagaaaag
     1441 ctcggctttt gatggtgaag ctgtcccgct gtcagtcgcc gcgtcttgcg cagctcaagg
     1501 tcgcggaggt tgccaaggct tggtcgagtc atcgctttgt cctttgcatc cgcagagcac
     1561 gggtttgcca caaaccgcgg atgctggcgg catggtatcg ggccgcgccg ccgagaggaa
     1621 gcccccgtca tcacctgtca cggaagcgtc acatccaggt cgacgcacgg actcgcgtgg
     1681 ctgcggaatt cggtacggaa caaccgcaat aaattcccat ggttatgttt cacaccgaaa
     1741 tctagcagcc tgatatcttg cgcgcagttt aggagtgatc atgcgcaaac gaactttctt
     1801 gtcttctgtc gttttgtaca cgcttgcggt ggcttcgcct gcgcttgcac agacttcgcc
     1861 tgatgcgacc gcgcccgctt ccccttcacc cagtgattca gcaggcacat cgtctgcgcc
     1921 atcgacggac ggttcgtctt tgggctcgtc ggggtctgcg cggtccatgt ccgcttctga
     1981 cggttccagc gacacgacgt cggatcgact caagcagcgg gaaaatggcg tggtggtttc
     2041 gctgggcctg accagccgca tgccggttgt gcaggtcgat ggcaccacgt ctgccgccgc
     2101 aaacggcatc ggtggcagcc tgatggccgg ttacaagctc aatcgcatca tcgtcggact
     2161 tggcttttca atcgatcact tcggtcaaaa gaccaagctc gtcgcaccgt cgggaacggc
     2221 gaccagcgtg accgatacca cgtcgttttt gatctcgccg acggttcagg tgggcatcct
     2281 gcgcgctgcc gccggacggc tggaactgta tggctcggcg cagtttggcc tgggccgcgc
     2341 attcctcagc cgcagcaatg atccgaccct gccgtccgac gtgcttgcga cctacgacaa
     2401 caaaaacttc catctcaact acgaggttgg tcctgggctg cgcttctact tcatcccgca
     2461 gtttgccctg cagcttacga gcggcgtcgc cggcgaccac ttcttcgcat cccaggacaa
     2521 tccatcggga cggcgcaccg acagcttgtc gagcgtgagc ctgtttggaa acctaggctt
     2581 gcttggagtc ttctaagtct gctttcggtc agtccacatt cattcctttg gaggcaaccc
     2641 atgcgacttc ttggcatcgt cggacttctc gcgctctcgg tcgggtgcga tgcaacacca
     2701 gcgagcactg ctccatcgac agcacgcgag tttctggcga cgtacaacca gatttcgtgc
     2761 gagtcccgtc tgcgctgctg cggcacggtc tgtagcgacg caagcgatga cagtttctac
     2821 aaaaactact ctcggacgct cgattacgta gcggctgggc tttaccggtt cgacccggcg
     2881 gcggcctcgg cgtgtcttgc gtcgtattcc gacttttaca agtcgtgcga taccgcgctc
     2941 accgatctgc cggtggtcag tgcccagtgt ggcgctgtca ttgtcggcaa cacccaggcc
     3001 ggcgggcagt gtgacagcag cctttcgccg agcaactgcg tggcggacac cacctgctcg
     3061 ggcggaacct gcatggcgaa ggccaaggtt ggcgaatcgt gcctgacttc aagctgtgtg
     3121 gcggcggcgt tctgcgatac gtcggtcgcc atgccggtct gcaaggcgcg gcttacggcg
     3181 ggccagacct gtacggccag ctcgcagtgc caaacgacgg cgttctgtga ttcgagtgtc
     3241 ctgccgcgtg tctgcaccga ctatggcaag gaaggccagc cgtgcagcgc atcgaagctg
     3301 tgtgatccca cgagcggcaa gctgtcctgc ctgccaaacc aagtctgtgg cggtccgcaa
     3361 aacgatggcg ccgcgtgtgc aagctcgacc cagtgcaaga gcggtcgctg cgcgggcgtg
     3421 gccccaaacc aagtctgcca ggcccagcca tcgccacaga cgcttcgcga tcagctctgc
     3481 ggtctgaagt agtcgcccgc cgttttcgcc cgccgttttt gcccgccctg atcccgccgg
     3541 aaattcgtgc gcgattgcgt gtcgcgcgtc tcccgctgcc aagcaacttt cggtaagcta
     3601 tgggaagctg ctctctcttc gcatctgctg tggcggtgcc ctgggggagc ggggggacga
     3661 aaacaccatg tcaggcaagc agcaggcgcg ctttgcgccg tcgggcaaca tcatcgtctg
     3721 cggacgcgcc tacaacattg gctggccggt cgtaaatttc tacgacaacc ccaacttcag
     3781 cgcctaccgg ctcggctgtt acaaaggcaa ggacggctcg ccgccgggcg ctttgccgtt
     3841 tggtcccgcc aagggtcttg gcaagtcacc gtatcgctac cgcgagcgtc gcttgatggg
     3901 tggccagcgc aacgacctgc gccgtctgca agacatcatc cgtcagtttg tgctgcacca
     3961 cgacggctgc gcgaccagtg ccgactgttt ccatgtgctc catgacgagc gtggtctgtc
     4021 ggcccacttt ctggtcgaca acaacggctg gatttatcag acgctggatc tgtccgacgg
     4081 cgcgtttcat gcgtcgggcg taaacgagat atccatcggc gtcgagctgt gcaaccgcgg
     4141 ccccgtcgag ctggacggcc cgcagtttta ctcaaagcgc ggcatttctc gcgatgtcac
     4201 cgaggtggtg gtccacaaca cccgctaccg catgtggcaa tacacccagg atcagtacct
     4261 ggcgatggcg gcgcttggca aagcgctggc gcgtctgctc ccgaacttgc cgcaggtgtt
     4321 ccccgagtgg aacggccaaa tcctcaacac ctggatggcc gacccgcgcg cgtttgccgg
     4381 ctacatcggc cactaccacg tcaccaacaa caagtgggat ccgggctgct ttgacttcaa
     4441 gtggctggcg cagaaaatcc gggttagctc gacgtggttt gtctgtttgg acaaaaacag
     4501 tgagtgtccc aagccgcccg aaatcgccga aaatccgcag ctagccgagc agcagggcca
     4561 agtcctgttt cgcaacaacg aagaagacgc gatggggggc tactttcccg tcgggccgtt
     4621 tgggcgctcg cggctgtggc acggcggcat tcacctgaac ttgccgcaag gtgcgccggt
     4681 gtttacgccg tttcccggac ggattgtggc ggcgcggttt ggcgatgatg ttccgacggg
     4741 gtctgccaac tttgtgctga tgcggcacta tttccgtctg gctggcgtcg agacgctgtt
     4801 tttcatcctc tacatgcacg tcgagcgcga ggaaccaaaa agcggacgca cgccgtggct
     4861 gtttgaggcc gacaaaaagc cgttcttttc ggcgatgtct tcgggcggcg tgttctatcc
     4921 ggacatcgac ttggccggcg gtgaaatgat aggccacgtc ggtcaggccg gtccgcccgg
     4981 caactacgaa agccaaatcc acgtcgaagt gatgtcgcct gaagacctgg ggtcgaagct
     5041 cgatgcgggg tatttttcgt cgattgatgg caagtcgagc ggtcacttct gcgacgtgcg
     5101 cgacatcgtt tcggcgatcg accgacccaa aggcaaaggc gacggcatcc tgaccgaaaa
     5161 agagctgcgc gactttttcc aaaacgacca gggcaagctg gagctgcgca agctggcggt
     5221 gcgctttcag tccgagtggg gcgacgccaa tgactgggaa cagcagctaa acctgagcaa
     5281 agactttcgc gcgatgccaa agccggtgcg cgccaagctg taccgcgacc agatcaaacc
     5341 gacgctgtgg tacacggcag agctggccga aaaagtcggt ctgcccaaag actttgtggt
     5401 ctggcactac catcccgtgc gctttatcgc gtggatgaac agccagcttc gcaagcaagc
     5461 gacgacgatg gtgggtcaga ttcaggtggc gcaggggccg gcggcagcca aagtgctcga
     5521 tgaccgtgaa tcgctcgaag gcttcaccga tgaagaagac gagctgtcgc ccgaagccag
     5581 tcgccggctc acgctcgaag acctggcagg cggctacccc gacgaatccc ccggcaagta
     5641 acacgcac
//
LOCUS       DAORGN010000261         5646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7043_length_5646_cov_7.087104, whole genome shotgun sequence.
ACCESSION   DAORGN010000261 DAORGN010000000
VERSION     DAORGN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_5646_cov_7.087104"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKL17_19665"
     CDS             complement(<1..582)
                     /locus_tag="PKL17_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HNK47011.1"
                     /translation="METTQVQEKRRVRLPVVGKAEESGVDVDAILREFEAEQRAKLGL
                     DKPREQWKDEIHQRWTASQRPHTTMLCGGLTMAQDFLISGALEGIGYRVVPLDVPDNS
                     ALQVGKEFGNRGQCNPTYFTVGNLIKHLLYLRDVKQIPTKEIIERYLFVTAGACGPCR
                     FGTYVTEYRKALRDAGFDGFRVLLFQQQGGLKQA"
     gene            complement(622..4281)
                     /locus_tag="PKL17_19670"
     CDS             complement(622..4281)
                     /locus_tag="PKL17_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNK47012.1"
                     /translation="MSVYVSPQSTQRKDPVMVGIDVGSTTVKAVVVDPQSREILWSDY
                     QRHQTKQPEFVLDFLRRIQDAFPLISPSAMRIFMTGSGASPIAPQISAKFVQEVNAVT
                     LAVETLHPDVGSVVELGGQDAKIIIFKQNDKTGDKQASTSMNDKCASGTGATIDKCIL
                     KVALPNEEVVKIQFDDSRLHHVAAKCGVFAETDIVNLVKSGIPAVEIMNSLADAIVMQ
                     NLSVLTRGNTLRPKVLLLGGPNTYLPFLRDCWRKRIPETWNDRGFPYPKDVPIEELII
                     VPQNAQYYAAFGSVMYGMHEAADVGRFIGVDKLTDWIATGRKSRLGDTAGAPLLRAPS
                     VDEKHAELEAFREQYRIPKFQKKQLEPGQKVRGVIGMDGGSTSSKAVLIDYDTAEILT
                     KEYQLSKGNPIQDTKEILARIQKYVADQNAELEVMGFGATGYAADVLQESVKSDCNIV
                     ETVAHMMSAVHFFGDVDVICDIGGQDIKVLFMENGDIKNFRLSNQCSAGNGMLLQAMA
                     DQFGVPITQYADVAFQADLAPKFSYGCAVFLDSDRVNFQKEGYAKEELLAGLAQVLPK
                     NVWQYVVQIPRMASLGKRFVLQGGTQHNLAAVKAQVDYIKDRVPGAEVFVHPHTGEAG
                     AIGAAMETLRVVKRRGKSSFIGLDAAINLTYTSTTDESTRCHFCPNLCQRTFIDTVTP
                     DGATSRYIAGFSCEKGTVESEEAMLAITKERKRLMKEFPNLVDFEAKKLFSHFYDAEP
                     TPTADTIIDDVEVKKGLFSVKRVPVRRPFQRSSEAAWQKRAAIRIGIPRVLNIWTIGP
                     FIRTYLESIGIQRHHVVFSDETTEEIWQEGGKYGSIDPCYPSKVAQAHIHNLLFHHHS
                     DKKRLHYIFFPCITHVPPGLHKVMDSASCPIVAGAPEVMKAAFTKEVDFFATRGIKYL
                     DPALTFTEAALLRRQMFECFGSLLDVTEDESDFAVEQAHKAMERFDQEIQKRGRAILE
                     QVEEDNRVAVLLIGRPYHSDPGLNHSVLEEFQVLGYPILSIRSIPKDPRWLHRFFKED
                     MEKGRIDGPLEVTDVWPENYSANSVQKVWAAKFAARHPNVVVLDLSSFKCGHDAPTYG
                     LVDSIISASATPYSALHDIDANKPGGSIKIRVKTYAHSLKLHEERLEDVAKKSAELHF
                     KIEEKRLALLELKQQQLEERKQQDPSLQAEIAELRKKVADYAARFTPQSTVEKDKSLV
                     RIGLKKKGEEQVVRL"
     gene            4542..>5646
                     /locus_tag="PKL17_19675"
     CDS             4542..>5646
                     /locus_tag="PKL17_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47013.1"
                     /translation="MKLRHTLPALAVALLVLGAASPSRAEDAMDQARKRYADGEREYA
                     SGRYWQSAKAFEEAFDLSRKGDLLFNAARAYDRGEYYVRAIETYEAYLKAGTQPDSEQ
                     INKRIADLRKQLSLVHLSTGEKAYVFVDGKEYYQTPMAGPAPIDGGYHRIDVRVGNRF
                     WAREQQFVPGEKYEFEVALEEDRGGGSGLTSMSQEVDIRAKPRTRQVAVVLGVGGTID
                     IFGNNFPPHQASLMIGAEYRAREGRYGAFDLGVKVPLEFLQSWQNAGLLLNLRGVLSP
                     VPRLPLELVLSLDVGFAAMSYTSAAPFSTKWPCATPSYLPSCTVYAMRAVPALSVAYR
                     FVPGFEMRAQLAGFELNVSQPMFDPRLAFGLQAA"
BASE COUNT         1126 a         1729 c         1551 g         1240 t
ORIGIN      
        1 cgcttgcttg agaccaccct gctgctggaa cagaagcacg cggaaaccgt caaagcccgc
       61 gtcgcgcagc gccttgcggt attcggtcac atacgttccg aaacggcacg gaccacaggc
      121 gcccgcggtg acaaacaggt agcgctcgat gatttccttg gtcggaatct gcttcacatc
      181 gcgcagatag agcaggtgct tgatcaggtt tccgaccgta aagtacgtcg ggttgcactg
      241 accgcggttg ccaaactctt tgccaacctg aagcgcgctg ttgtcgggca catccagcgg
      301 caccacgcga tagccaatgc cttcgagcgc gcccgagatc aggaagtcct gcgccatcgt
      361 cagaccacca cagagcatcg tggtgtgcgg acgctgcgac gcagtccagc gctgatgaat
      421 ctcgtctttc cactgctcgc gcggtttgtc gagtcccagc ttggcgcgct gctcggcttc
      481 aaactcacgc aaaatggcat ctacgtccac acccgattct tccgcctttc cgacgacagg
      541 caaccgaacg cgacgctttt cttgcacctg ggtggtttcc atttcctctg cttctccctc
      601 gtaagtgaaa aaggaacgcg gttacaggcg gacgacctgc tcttcacctt ttttcttgag
      661 cccaattcgc accaggctct tgtctttttc gacggtactt tgcggcgtaa agcgcgctgc
      721 gtagtccgcg actttcttgc gcagctcggc aatctccgct tgcagactcg gatcttgctg
      781 cttccgctcc tcaagctgct gctgcttcag ttccagaagc gcgagccgct tttcttcgat
      841 cttgaagtgc agctcggcgc tctttttggc gacgtcctcg agccgctcct catgcagttt
      901 cagactgtgg gcatacgtct ttacgcgaat cttgatcgat ccgcccggct tgttcgcatc
      961 aatgtcgtgc agcgcggaat atggggtcgc gctcgccgag atgatcgaat ccaccagtcc
     1021 gtaagtcggt gcatcgtggc cgcacttaaa cgacgacaga tcgagcacca cgacgttcgg
     1081 atgacgcgcc gcaaacttcg ctgcccacac cttctgcacc gagtttgccg aatagttttc
     1141 cggccacaca tccgtcactt ccagcgggcc atcgatgcga cctttttcca tgtcttcttt
     1201 gaagaagcga tggagccagc gcgggtcttt gggaatcgat cgaatcgaca gaatcggata
     1261 cccaagcacc tggaactctt cgagcacgct gtggtttagg cccggatcgc tgtgatacgg
     1321 acgaccaatc aagagcaccg caacgcgatt gtcttcttcg acctgttcca gaatcgcgcg
     1381 accgcgcttt tggatttcct gatcgaatct ttccatcgct ttgtgcgctt gctcgacggc
     1441 aaaatcgctc tcgtcttccg tcacatcaag cagcgagcca aagcactcga acatctgccg
     1501 ccgcaaaagc gccgcttccg taaacgtcag tgctggatcg agatacttga ttccgcgggt
     1561 cgcaaaaaag tcgacttctt tggtaaacgc ggccttcatg acttcgggcg caccagcgac
     1621 gatcggacac gacgccgagt ccatcacttt gtgcagtccg gggggcacat gggtgatgca
     1681 cgggaaaaag atgtagtgaa ggcgcttttt gtcgctgtgg tggtgaaaca gcagattgtg
     1741 aatgtgcgcc tgcgcgacct tggacggata gcacggatcg atcgatccgt acttgccacc
     1801 ttcttgccaa atctcttccg ttgtctcgtc ggaaaagacg acatggtgcc gctggattcc
     1861 gatcgattcc agatacgtcc ggataaacgg accaatggtc cagatgttga gcacgcgcgg
     1921 aatgccgatg cgaatcgcag cacgcttttg ccacgcggct tcggagcttc tttggaacgg
     1981 acgccgcacc ggaacgcgct tgacgctaaa cagtcctttt ttgacttcca cgtcgtcgat
     2041 gatggtatcc gccgtcggcg tcggctccgc atcgtagaag tgcgaaaaca gcttctttgc
     2101 ttcgaagtcg accaggttcg ggaattcctt catcagccgc ttgcgctctt tggtgatcgc
     2161 aagcatcgct tcttccgatt cgacggttcc cttttcacaa gaaaagccgg cgatgtagcg
     2221 gctcgtcgcg ccgtccggcg tcaccgtgtc aatgaaggtg cgctgacaca ggttcggaca
     2281 gaagtgacag cgcgtcgact cgtcggtggt cgatgtgtac gtaagattta tcgccgcatc
     2341 aagaccgatg aacgagcttt tgccacgacg cttgaccacg cgcagcgttt ccatcgccgc
     2401 accgatcgcg ccggcttctc cggtgtgggg gtgaacgaat acttcggccc ccggaacgcg
     2461 atccttgatg taatccacct gcgctttgac ggcggccaga ttgtgctgcg taccaccctg
     2521 caaaacgaag cgtttgccga ggctggccat gcgcggaatc tgcaccacgt actgccacac
     2581 gttcttggga agcacctgcg caagacccgc aagcagctct tctttggcgt agccttcctt
     2641 ttggaagttc acgcgatcgg aatccaaaaa cacggcgcag ccgtaactaa acttcggtgc
     2701 cagatcggct tggaatgcga cgtcggcata ctgcgtaatc ggcacgccga actgatccgc
     2761 catcgcttgc aggagcattc cgtttcctgc cgaacactgg ttggaaagac ggaaattttt
     2821 gatgtctccg ttttccataa acagcacttt gatgtcttgg ccgccgatgt cgcagatgac
     2881 atcgacatcg ccaaagaagt gcaccgcaga catcatgtga gcgacagtct cgacgatgtt
     2941 gcagtcactc ttgaccgatt cctgaagtac atcggcagcg tatccggtcg cgccaaagcc
     3001 catcacttcc agctcggcgt tttgatcagc aacgtacttc tgtatccgcg ccaggatttc
     3061 ttttgtgtcc tgaatcggat ttcccttcga gagctgatat tccttggtca aaatttccgc
     3121 ggtgtcgtag tcgataagga ccgccttcga tgaagtcgat ccaccgtcca ttccgatcac
     3181 gccgcggact ttctgtcccg gttcaagctg cttcttttgg aacttgggaa tccgatactg
     3241 ctcgcggaat gcttccagtt cggcgtgttt ttcatcgacg gacggcgcac gcaaaagcgg
     3301 agcccccgcc gtgtccccaa gccgcgactt gcgtccggtc gcaatccaat cggtcagttt
     3361 gtcgacgccg atgaagcgac cgacatctgc cgcttcgtgc attccgtaca tcaccgaacc
     3421 aaacgccgcg tagtactgcg cgttctgcgg aacgatgata agttcctcga taggaacgtc
     3481 tttgggatac ggaaagccgc ggtcgttcca cgtttcagga atccgcttgc gccagcagtc
     3541 ccgcaaaaac ggcagataag tattcggacc gccgagcaga agaactttcg gacgcagcgt
     3601 gttgccacgg gtcagcaccg acaggttctg catcacgatc gcatcggcga gcgagttcat
     3661 gatctcgacc gccggaatgc cgctcttgac caggttgacg atgtcggttt ccgcaaacac
     3721 gccacacttg gccgcgacgt ggtggagccg gctgtcgtca aactgaatct tgaccacttc
     3781 ttcgtttggc agcgcgactt tgaggatgca cttgtcgatg gtggcgcccg taccgctcgc
     3841 gcacttgtcg ttcatcgacg tcgacgcctg cttgtccccc gtcttgtcat tctgcttaaa
     3901 gatgatgatc ttggcgtctt gaccgcccag ctcgaccacc gatccgacat cgggatgcag
     3961 cgtttcgacc gcgagcgtca ccgcgttcac ttcctgcacg aacttggcgc tgatctgcgg
     4021 agcgatcggc gacgcgcccg atccggtcat gaagatccgc atcgcgctcg gtgagatgag
     4081 cgggaaggca tcttggatgc ggcgcagaaa gtcgagcacg aactcgggct gcttggtctg
     4141 gtggcgctgg tagtcgctcc acagaatctc ccggctttgt ggatcgacga ccacggcctt
     4201 caccgtggtg gaaccgacat cgattccgac catcaccgga tctttgcgct gcgtgctctg
     4261 tggcgagaca taaacagaca tgcggtcctg ctccttcgac gagatcgcta tcgttccgcg
     4321 cgcggcgggt tgccaaaacg accaattaga ctaccagtac agtctatgcg cagacgttac
     4381 gcgccgccgc atggatcggt caacaaaaaa ccaaacgcag ggccggaccc caaaagccaa
     4441 agtgccgcga cgcaaaatgg tcgagccaaa aacgatcgcc tggcaccgct cacgtcgaac
     4501 cagtgactat ttttttgtaa tcttcgcggt atattgatga tgtgaaactc cgccatactc
     4561 tgcccgccct ggctgtggca ctgcttgtgc tcggggccgc atcgccaagt cgcgccgaag
     4621 acgcgatgga ccaagcccgc aagcgctacg ccgacggaga aagagagtac gccagcggtc
     4681 gctactggca gtccgccaaa gcgttcgagg aagcgtttga cctgtcgcgc aaaggcgacc
     4741 tgctgttcaa cgccgcccgc gcctacgacc gcggtgagta ctacgtccgc gccatcgaga
     4801 cctacgaagc gtacctgaaa gccggcaccc agcccgactc ggaacagatc aacaagcgca
     4861 tcgccgatct gcgcaaacag ctgagcctgg ttcacctgtc aaccggcgaa aaagcctacg
     4921 tcttcgtcga cggcaaagaa tactatcaga ctccgatggc cggcccagcc cccatcgacg
     4981 gcggatacca ccgcattgat gtccgcgtcg gcaaccgctt ctgggcccga gaacagcagt
     5041 ttgttcccgg cgaaaaatac gaattcgagg tcgctctcga agaagaccgc ggcggtggtt
     5101 cgggcctgac cagcatgagc caagaggtcg acattcgggc caagccacgc acccgccaag
     5161 tcgcggtcgt gctcggtgtc ggtggcacca tcgacatctt tggcaacaac ttcccgcccc
     5221 accaagccag cctgatgatc ggggctgaat accgcgcgcg cgaaggtcgc tatggcgctt
     5281 ttgacctggg cgtaaaagtt ccgctcgagt ttcttcagtc gtggcaaaac gccggtctgc
     5341 ttctgaacct gcgcggggtg ctttcaccgg tgccgcggct gccgctcgag ctggtgctgt
     5401 ctttggatgt cggctttgcg gcgatgtcct acacgtctgc agcgccgttt tccaccaagt
     5461 ggccctgtgc aaccccgtcg tatctgccaa gctgtacggt ctacgcgatg cgcgcggtgc
     5521 cagcgctgtc ggttgcgtac cgctttgtcc ccgggttcga aatgcgcgcc cagctggccg
     5581 gcttcgagct caacgtgagc cagccgatgt tcgatccgcg tctggctttt gggctccaag
     5641 ccgcct
//
LOCUS       DAORGN010000262         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7147_length_5598_cov_5.937218, whole genome shotgun sequence.
ACCESSION   DAORGN010000262 DAORGN010000000
VERSION     DAORGN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7147_length_5598_cov_5.937218"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PKL17_19680"
     CDS             complement(<1..970)
                     /locus_tag="PKL17_19680"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNK47014.1"
                     /translation="MTMPRTLPLPMVLQSFLWIRYPVQVMNYCRKEFGPTFMVRLPRF
                     QMVMLTEPDAIRTMFASKGDDLHAGKVNTILRAIVGSSSVLLLDGAEHMRHRKLLLPS
                     FHGERMRFYGETMADIARQSIATWPDHDVFALHEKTQDITLQIILRTVFGVDEGTQMH
                     KLAAQLTRLLRLGENPLFLPVMVALAQDPTLEKRMPWKWILRDRDETDALLFKQIAAR
                     RAEPESERSDVLAMLLKARDEKGQGLSDQELRDELITALVAGHETTATGLAWAFERIL
                     LHKPVYDRLKQEVRDAGGVHATPEKLAALPYLDATIKEVLRLRPVIP"
     gene            1086..2240
                     /locus_tag="PKL17_19685"
     CDS             1086..2240
                     /locus_tag="PKL17_19685"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNK47015.1"
                     /translation="MSKRLAALDIQRGFIMMLMALDHVVAFVYRYHPSEVWAGAWTRY
                     SSWERFVTRLVTHLCAPGFFLLMGAGVALFLEARRRDGWTDDKARSFLLKRGLLLLLI
                     NQVIENRAWSIGFMTSQAPPPAVPDGPWPGTPGPMLLVFSVLSGLGMALVLASLLLRL
                     RTRWLAICATALVLASTVCTPSPDHLQQYYSLPMRLLFLPGQTGVAFVLYPLIPWSGL
                     ALWGLVLGRSLHEKSDATLAKLPWIGLLMVVLALVLRALGGPGNLRLPRDSSVIEFFN
                     LIKYPPSLVFLLFFVGINLLLLSFWHRVSGKIGRLADILATFGQVPLFYYLLHLFLYC
                     FVGALWFRRGGSLAMTYVVWLLGLIPLYVSCLLYRRFKSKTAVDSIWRFF"
     gene            2376..5225
                     /locus_tag="PKL17_19690"
     CDS             2376..5225
                     /locus_tag="PKL17_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNK47016.1"
                     /translation="MQLLLEMVRSRQADSPFAFQLGPQQYVLRTALGGAETARLDWDA
                     ELLDDLIALRSPNPSAERIQRMGARLRQFLMTTSFGQSEAAILDAVGRGEAVQVTLRF
                     AASELFALPWELLTIGASGVQLGALPSLLLRYAWPQTQTAQPTSPTPASESGRILFAW
                     SAAGGAVPAADQVVAVAQAAKRGQIPFDSQRDVISHLSPQRLVRALSGEVGQPPVAVL
                     HILCHGAEQSGSFGLVWNDEEGDEIFVDAAQLRLLLAPYASTLRLVVLSSCDSGNSGT
                     LGSHLGSVAQALHRMGIATVLASRYPLSVASSLRLTQQLYDSLLYDLCSVEQAFVEVR
                     KQLVQASSRLDWAALQLYARPEDGDDNRPILFRPYLGLAAFRMGQSRFLFGRDEERWA
                     ARQKLASLSSRGQPRFLVVTGASGTGKSSVVLGGLLPDLVQPSTNQPQQSALQKTAAE
                     LQRLLSSLPQKTQTQALRDALAQVAQELDRLPIHPSTGGWQWAVLRPGVCPLRSLDDA
                     LACRQETTKPFLLVVDQFEELFTQCTDEPQRSAFVRKLWDLAQCADDISVVVTLRVDF
                     LGRCGELFLSDSGLALDRIAYADEHRVFVAKPTAEQLMQAIRGPAQLLGLSIAPALVQ
                     RMLSDVVGEPGALPLVSYVLDLLWLRRDGRRLSETVYADLGGVAGALGQSADLLYASL
                     DPTEQRLARQLLVRLVHVGSEIGGETRRRVRLSDLRHTLSDPQRLLDGVIARFVDARL
                     LVRDEESGAAVIEFAHESLIRRWSLLRGFLLEDRQRLVELTELSQWVTQYRDYGTLLR
                     GAQLGYAERLADKYPDELGEDAQNLVSSSLRAARRRRWLLTAMVIFVSVSLSALSLRA
                     HRSELRAQAEKREAQSKTRTAQARLLGQHAKLTGFPSRTSAEGQGLVLIWSICLVCGI
                     AGMPTGLRHTGRVVDGVFLQRHDLTESRDFRAQGY"
     gene            5379..>5598
                     /locus_tag="PKL17_19695"
     CDS             5379..>5598
                     /locus_tag="PKL17_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47017.1"
                     /translation="MGDVHNRGTKDRPQWYCRYVDLDGKRKHRPTHQPSKALAMRFVA
                     EVEARVARGLVGIPEKHSPERQGKALTLL"
BASE COUNT          923 a         1636 c         1765 g         1274 t
ORIGIN      
        1 acggaatcac cggacgcagg cgcaggactt ctttgatcgt tgcatccaga tacggcagcg
       61 ccgccagctt ttcgggcgtg gcgtggaccc cgccagcgtc gcggacttcc tgcttgagcc
      121 gatcatagac cggcttatga agcaggattc gttcaaacgc ccacgcaagt ccggtcgctg
      181 tggtttcatg tccagccaca agcgccgtga taagttcatc gcgcagctct tgatcgctga
      241 gcccttgtcc cttttcatcg cgggctttga gcagcatggc cagcacatcg ctgcgttcgg
      301 attctggttc ggcccgtcgc gccgcaatct gcttaaacag cagcgcatcg gtttcatcgc
      361 gatcgcgcaa aatccatttc cacggcatgc gtttttccag tgtgggatct tgggccagcg
      421 cgaccatcac cggcaaaaac agcggatttt ctcctaggcg cagaagccgc gtaagctgtg
      481 ccgcgagctt gtgcatttgg gttccttcat cgacgccaaa caccgtgcgc aagatgatct
      541 ggagcgtgat gtcctgcgtc ttttcgtgca gtgcaaacac atcgtgatcg ggccatgttg
      601 cgatcgactg gcgcgcaatg tccgccatcg tttcaccata aaaacgcatc cgttcgccat
      661 gaaacgaagg cagcagcaac ttgcgatgac gcatgtgctc tgcaccgtcg agaagtagca
      721 ccgaagaact gcccacaatc gcccgcaaaa tcgtgttcac tttgccggcg tgcaggtcgt
      781 cgcctttcga tgcaaacatg gtccgaatcg cgtccggttc cgtcagcatc accatctgga
      841 aacgtggcag tcgaaccata aacgtcggac cgaattcctt ccgacagtag ttcatgactt
      901 gcacggggta gcgaatccac aaaaaggact gaagcaccat cggcagtggc agtgtgcgcg
      961 gcatcgtcat ggtaaaaagg atgcaccaaa agcggaagcg atgcgaagtc agtttcaaat
     1021 cctggatgtc gttcctttgt tcgcattccg cgaaaaccca atcgctcggc ggagggattc
     1081 ctagcatgtc aaaacgactg gcggcgctcg atatccagcg tggttttatc atgatgctga
     1141 tggcgctcga tcatgtggtg gcatttgtct atcgctacca tccatcggaa gtctgggcag
     1201 gggcatggac gcgctattcc tcgtgggaac gctttgtcac ccggcttgtc acccatctgt
     1261 gcgcccctgg tttttttctg ctgatggggg ccggcgtggc gctgtttttg gaagcgcggc
     1321 gtcgcgacgg ctggacggat gacaaggcgc gcagcttttt gttaaagcgc ggacttttgc
     1381 ttctgctcat caatcaggtg attgaaaacc gcgcttggag catcggcttt atgacaagcc
     1441 aagcgccgcc ccctgcggtt cccgacggac catggccggg aacgccgggt ccgatgttgc
     1501 tggtgttttc cgttctgagc ggtctgggaa tggcgcttgt tttggcctcg ctgctcttgc
     1561 gcttgcgcac gcgctggctg gcgatctgtg caactgcttt ggtcttggca tcgacggtct
     1621 gtacgccgtc ccccgatcac ttgcagcaat attacagctt gccgatgcgt cttttgtttc
     1681 tgcctggtca gacgggagtg gccttcgttc tgtatccgct gattccctgg agcggtcttg
     1741 cgctgtgggg tctggtcctg ggccggagtc tgcatgagaa atccgacgcg acgctggcga
     1801 agctgccctg gattggtctt ttgatggtgg tcttggcgct tgttctgcgc gctttgggag
     1861 gccctgggaa tctgcgccta ccgcgcgaca gttcggtgat cgaattcttc aacttgatca
     1921 agtacccacc gtcgctggtg ttccttctgt tctttgtcgg catcaatctg cttttgctgt
     1981 ccttttggca ccgggtcagc ggaaaaatcg gacggctggc cgacattctg gcgacgtttg
     2041 gacaggtgcc gctgttttac tatctgcttc acctgtttct gtactgcttc gtgggtgcgc
     2101 tgtggttccg tcgcggtggg agtctggcca tgacctatgt ggtgtggctt ttgggactga
     2161 ttcctttgta cgtgtcgtgt cttttgtacc gccgcttcaa gtcgaagacc gccgttgatt
     2221 cgatctggcg ttttttctaa gcccgcacaa agcgggttcc tgcgcagctt gcgcgatcca
     2281 gcctttccca cgccggttgt cgtttttttc aacaacaggc gtgcagccaa gccacacgca
     2341 ccccgctcgg ggtcgctgtt atcgtcgaca aggcgatgca gcttctgctc gaaatggtcc
     2401 ggagccgtca ggccgattct ccgtttgcgt ttcagcttgg accgcagcag tacgtgctac
     2461 ggactgcgct gggcggtgca gagacggcgc ggctcgactg ggatgcggag ctgcttgatg
     2521 atctgatcgc gctgcgcagt ccgaatccgt cagcggagcg gattcagcgc atgggagctc
     2581 ggctgcggca gtttctgatg acgacgagct ttggccaaag cgaagcggcg atcttggatg
     2641 cggtcgggcg gggcgaagcg gtgcaagtga cactgcgttt tgcggcgtcg gagctgtttg
     2701 cgctgccatg ggaattgctg acgatcgggg cgagcggagt ccagctcggc gcgcttccgt
     2761 ctttgctcct tcgctatgcg tggccacaga cccagactgc gcagccgaca agcccgaccc
     2821 ctgcaagcga gagcggacgg attctgtttg cgtggtcagc ggcaggtgga gcggtgccgg
     2881 cggcggatca ggttgttgcc gtcgcccagg ctgcaaagcg cggccagatt ccgttcgatt
     2941 cccagcgcga tgtcatctcg catctgtcgc cgcagcgtct ggttcgtgcg ctcagtgggg
     3001 aagtcgggca gccgccggtc gcggtgcttc acatcctgtg tcacggtgcc gagcagtccg
     3061 gttcgtttgg tcttgtctgg aacgatgaag agggcgacga aatcttcgtc gatgcggccc
     3121 agcttcgtct gctcctggcg ccgtatgcca gcacgctgcg gctggtcgtg ctgtcgtcgt
     3181 gtgacagcgg caacagcggt acgctgggca gtcatctggg cagcgtcgcg caggccctgc
     3241 accggatggg aattgcgacg gttttggcat cgcgttatcc gctcagtgtg gcgtcgtcgc
     3301 tgcggctgac gcagcagctt tacgattcgc ttttgtatga cctgtgttcc gtcgagcagg
     3361 cgtttgtcga agtgcgaaag cagcttgtgc aggcttcgtc gcggctcgac tgggcggcgc
     3421 ttcagctgta cgcgcggccc gaagacggtg acgacaaccg accgattctg tttcggcctt
     3481 atctgggact tgcggccttt cgcatggggc agagccgctt tctgttcggg cgcgacgaag
     3541 agcgctgggc ggcacggcag aagttggcca gcctttcgtc gcggggtcag cctcgttttt
     3601 tggtcgtcac cggcgcgtcg ggaaccggca agtcgtccgt cgtgctcggc ggacttttgc
     3661 ccgacctggt gcagccgtcg acgaaccagc cacagcaaag cgcgctgcaa aaaactgccg
     3721 ctgaacttca gcgtctgctt tcgtcgctgc cgcagaaaac gcagacccaa gcgctgcggg
     3781 atgcgctggc ccaggtcgcg caggagcttg acagactccc tatccatccg tcgacgggcg
     3841 gctggcagtg ggcggtgctg cgtcccggtg tttgtccgct gcgcagtctc gacgatgcgc
     3901 tggcgtgtcg tcaggaaacg acgaaaccat ttctgctggt cgtcgatcag ttcgaggagc
     3961 tgttcaccca gtgtaccgac gagccacagc ggtctgcgtt tgtccgcaag ctgtgggatc
     4021 tggcccagtg cgccgacgac atttccgtgg tggtgacgct gcgggtcgat tttttggggc
     4081 gctgcggcga gctgttctta agcgacagcg gacttgcgct tgatcgaatt gcctatgccg
     4141 acgagcatcg tgtgttcgtc gccaagccca ccgccgagca gttgatgcag gcgatccgcg
     4201 gaccggccca gctgctgggt ctttccattg cgccggctct tgtgcagcgc atgctaagtg
     4261 acgtcgtcgg tgaaccgggg gcgctgccgc tcgtgtccta tgtgctcgat ctgctttggc
     4321 tgcgccgcga cgggcgacga ctatcggaaa cggtctatgc cgatctcggg ggcgttgcgg
     4381 gggcgctcgg acaaagcgcc gatctgctgt atgcatcgct cgatcccacc gagcagcgtc
     4441 tggctcggca gctgcttgtg cggcttgtcc acgtcggatc ggaaatcggc ggcgaaaccc
     4501 gtcgtcgcgt ccgtctgtcc gatctgcgcc acaccctgtc cgatccacag cgtctgctcg
     4561 acggcgtgat tgcacgcttt gtcgatgcgc ggctcttggt gcgcgacgaa gagtccgggg
     4621 ccgcggtcat cgagtttgcg cacgagtcgc tgatccgtcg ctggtcgctt ttgcgcgggt
     4681 ttttgctgga agaccgtcag cggctcgtcg agctgaccga gctttcgcag tgggtgacgc
     4741 agtaccgtga ttacggcacg ctcttgcgcg gtgcccagct cggttacgcc gagcggctcg
     4801 ccgacaaata tcccgacgag ctaggcgaag acgcgcaaaa tctcgtctca tcgagcctcc
     4861 gcgcagcgcg tcgtcgtcgc tggctgctga ctgccatggt gattttcgtc agcgtgtcgc
     4921 tgtccgcgct cagtctgcgc gcccatcgca gcgagctgcg cgcccaagcc gaaaaacgcg
     4981 aggcccaatc caaaacccga accgcccaag cccgcctcct tggccagcat gccaaactga
     5041 ccggtttccc gtcgagaacg tcagccgaag gtcagggttt ggtccttatt tggtccatat
     5101 gcctggtctg cggcattgca ggaatgccga ccggccttcg gcatacgggg cgtgtcgtag
     5161 atggcgtgtt tctgcaacga cacgacttga ctgaaagtcg cgactttcgc gcgcagggct
     5221 actgaccgtc caaacggaat cgctcgccca gccggatgta tggactgtcg tctacggaag
     5281 cgttgatgcg ggcttcctaa aatccgagcg atcttgctat cctgcactct gttagactgt
     5341 gtccatgcga cgtggcgcgg tccgggggcg aggtgttcat gggagacgtt cacaatcgag
     5401 gcaccaaaga ccgcccgcag tggtactgcc gctacgtgga cctcgacggc aagcgcaagc
     5461 atcgtccgac acatcagccg tcgaaggcgc tggcgatgcg gtttgttgcc gaggtcgagg
     5521 cgcgggtggc ccgcggtctg gtcgggattc ccgaaaagca ctcgccggag cggcaaggca
     5581 aggcgctgac gctgctcg
//
LOCUS       DAORGN010000263         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7185_length_5585_cov_6.452984, whole genome shotgun sequence.
ACCESSION   DAORGN010000263 DAORGN010000000
VERSION     DAORGN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_5585_cov_6.452984"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKL17_19700"
     CDS             complement(<1..382)
                     /locus_tag="PKL17_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNK47018.1"
                     /translation="MASRNAPTDKPLVWLHGEVKKPPWSLEARREAGFLLRRLQLGDM
                     LSLPSSRPMPSIGPAVHELRIKDAGAEWRIIYRLDSDAIVTAVVFHKKTQATPHAIIE
                     TCKRRLRRYDEAAEQPGEQEAERMR"
     gene            complement(423..737)
                     /locus_tag="PKL17_19705"
     CDS             complement(423..737)
                     /locus_tag="PKL17_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNK47019.1"
                     /translation="MDKAKQKRLEAAGWKVGTVQDFLDLSPEEALLIDIRARLALALS
                     ERRKAQSLTQAELARRIRSSQSRVAKMEAADGSVSLDLLVRSLLALGTTRKELAAAIA
                     GK"
     gene            complement(885..1136)
                     /locus_tag="PKL17_19710"
     CDS             complement(885..1136)
                     /locus_tag="PKL17_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47020.1"
                     /translation="MVLSRLVLVLMLGLGLSACGDKSCKNACDKLSSCGLKSSGFSCD
                     ATCVSPEDTCAVCLNATACSDIASGKCAADCPKATFTSK"
     gene            complement(1164..2609)
                     /gene="lpdA"
                     /locus_tag="PKL17_19715"
     CDS             complement(1164..2609)
                     /gene="lpdA"
                     /locus_tag="PKL17_19715"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNK47021.1"
                     /translation="MQNIRTEVCVIGSGPGGYVAAIRAGQLGKKVVCVEKAAPGGVCL
                     NVGCIPSKAMISAAKQWEKLSHLSTMGISVSGQSLDAGKLQDWKSSIVDKLTSGVRQL
                     IKSAGGQFVSGTATLRDKNTIEVSGPDGNVTITADHIILATGSRPITVPGFTVDNQRI
                     HDSTGGLSFRSVPRRLTVIGGGYIGLELGIMWRKLGAQVTVVEFTDQLLPGSDPELVA
                     VVARKLKKLGVEVYLQHKALRYEESDGALKLWIVPRNADGQKDKELAIATDHILVTVG
                     RRPNSESLGLEQVGVSVDQRGFIVVDKQQRTSVPNIFAIGDVCGQPMLAHKASREGEV
                     AAEVIAGHRAEQDAVAIPAVIFTDPEIGSVGLSEQQAIEKGHKVLVGKYSFAALGRAM
                     AALETDGFVKVVADAATQQVLGVHIVGPAATDLISEGAFALEMGAFLPDLALTIHPHP
                     TLGEAIMEAAKAALGESPHVVGGGQRGATRH"
     gene            complement(2663..5542)
                     /locus_tag="PKL17_19720"
     CDS             complement(2663..5542)
                     /locus_tag="PKL17_19720"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNK47022.1"
                     /translation="MLKPRPFRRAVSSPLSFLLVLGLLFSLLRPAGAVGELGGSIGGF
                     VTIKGTKDGLASVPISATSKQLIGGAQQVVTGDDGSYSFVNLPPGTYELQIAMEGFAP
                     IKQVGIRVNAGQRSSVDIELEIGSAESTQQTTKIIEKVNPILNAESAAAVTPVSNQQV
                     TRAPTFRQEKAIAQFTAGVTSGTDKVAVRGGLGRFNRYFIDGLEVTDITLGAFGSSSA
                     LINSDSVEQFVISVGAMDAEYNSLGLVQNMVTRSGGNQFTVDATAILQPPFMAALTRY
                     PTRAPLQNTTLLYDDRPIPDRSYYSGAVNFGGPIIKDRLWFWTSFQASFNRITNSIAE
                     QPWHQIPSSYDRYQDQILYLGRVKLTWQATKATRVTLSYSTDFNDILNASTSSALRGI
                     DPNTLAPEAERRVQRGGHWVGLLVDSLVTDKLLFQLQTGVSYKNYSEDTLRKVAGVPD
                     RLTPSHVLQTADAATNNFVYLNGNRPWDEQGKWNVQFAPTLMYTARGLGGTHNIKAGF
                     QLSYMKYDHRVGIAGGQRFLDNTPGLPCDPQDVRTYGSCNQVETFPESLPGDGSAGAG
                     YLTKSQAINAGVFIQDRYTVGKWLTIVPGLRIDTGYLFDVSGARIQTLLGFGPRLSFV
                     YDLLHDHTTLLKVHYGRHNDLGNAGIADYTNPTQTSVLSRWNPTTSMFDEVRRSGGQG
                     SQRFATDLNMTPPSVDEVSAGVHRQVLDEAVVGIDYTFRHYGHLWVNQEVNLIWDPAG
                     TRIVGYANGQRERIFVADTPSEAQRTYHGVDLWVRGNPGNWDIMASYTLAFLNGTVND
                     FFESNGFGSNPRLNPLYDGPIAGAYRHYLKALVDYSFDFGLTIGGRLQYYTGLPQWKV
                     FRSPEDQTYTLYRSPRGSSTGTRVNDPTTWASFNLPDTFNLDLQVAYGLKKLTGVNLD
                     LLLMMFNALNLSPAQGIENRDGATFGQVTLRPDVFFAEFVIRYRY"
BASE COUNT         1046 a         1693 c         1720 g         1126 t
ORIGIN      
        1 acctcatccg ctccgcttcc tgttcgccgg gctgctcggc tgcttcatcg tagcggcgga
       61 gtctccgctt gcaggtctcg atgatggcgt gcggcgtggc ttgggtcttc ttgtggaaca
      121 ctacggctgt cacgatcgcg tcggagtcga gccgatagat gatccgccac tctgcgcctg
      181 catcttttat ccgtagctca tgcaccgccg ggccgatcga gggcatgggc cgcgacgaag
      241 gaagcgacag catatcgcca agctgcaagc ggcgtagcag gaacccagcc tcgcggcggg
      301 cttccagtga ccagggcggc ttttttactt cgccatggag ccaaaccagg ggtttgtcgg
      361 tcggtgcgtt tctcgatgcg atcggcacga tgggaaggcc acgagggggc cggggtggat
      421 gtttacttgc ctgcgatggc ggcggctagt tctttgcgtg tggtcccgag cgcgagcagt
      481 gagcgcacca gcagatcgag cgacaccgac ccatcggcgg cttccatctt ggcgactcgc
      541 gattgactcg accggatgcg ccgggctagc tccgcttgag tgagggactg cgccttgcga
      601 cgctcggaca gtgccagcgc caagcgggcg cggatgtcga tcagcagcgc ttcctccggt
      661 gacagatcga ggaagtcttg caccgtgccg accttccatc ccgctgcctc taatcgtttc
      721 tgcttcgctt tgtccatggg cgctgtctcc ggttccgagt atgtcagaaa tgacatagga
      781 cgcaagcggg ccgggctgcg cggccaaggt ctacaggttg gccggttggc cggtctgggc
      841 ggcaaccggg tggtaagcgg cccaaggccc gcggagggag cgagctactt gctggtgaag
      901 gtggctttgg ggcagtccgc agcgcacttg cccgaagcga tatcgctgca agcagtggcg
      961 tttaggcaaa cggcgcaggt gtcttcgggg cttacgcagg tcgcgtcgca cgaaaagcct
     1021 gacgacttga gcccgcagct tgacagcttg tcacaggcat ttttgcagct cttgtcaccg
     1081 caggccgaaa gcccgagccc gagcatcagc acgagtacca gtcgagagag aaccatcgtg
     1141 gaccaccttt cttttggaac cgtctagtga cgagttgcgc cgcgctggcc accaccaacg
     1201 acgtgcggtg attcgcccag cgccgctttc gccgcttcca tgatggcttc gcccagcgtc
     1261 gggtgtggat gaattgtcag cgccaggtcg ggcaaaaatg cgcccatttc cagcgcaaaa
     1321 gcaccctcgc tgatcagatc cgtcgctgcc ggaccgacga tgtgcacacc gagcacttgc
     1381 tgggtggcag cgtccgcgac gactttgaca aagccatcgg tttcgagcgc ggccatcgcc
     1441 cgtccaagcg ccgcaaatga atacttgccg acgagcacct tgtgcccttt ttcgatcgcc
     1501 tgctgctcgc tcagtccaac cgagccaatt tcgggatcgg tgaaaatgac ggcaggaatc
     1561 gccacagcat cttgctcggc ccggtgaccg gcgatgactt ccgcggcgac ttcgccttcg
     1621 cggctggctt tgtgggcgag catcggctga ccgcagacat cgccgattgc aaagatgttc
     1681 gggacgctgg tgcgctgctg tttgtcgacg acgatgaagc cgcgctgatc gacagaaacg
     1741 ccgacttgct caagtcccag gctctcgctg ttcgggcggc gtccgacggt gaccaggatg
     1801 tgatcggtcg caatcgccag ctctttgtct ttctgtccgt cggcattgcg cggcacgatc
     1861 cacagcttca gcgctccgtc gctctcctcg taccgcaggg ctttgtgctg aagatacact
     1921 tcgacgccca gctttttgag cttgcgtgcg acgaccgcca caagctcggg atcgcttccg
     1981 ggcagcagtt gatcggtaaa ctcgacgacg gtcacttgcg cgccgagctt gcgccacatg
     2041 atgccaagct caagaccgat gtagccgccg ccaatcaccg tcaggcgacg cggcaccgat
     2101 cgaaagctga gcccgccggt cgagtcgtga atgcgctgat tgtcgacagt aaagcccgga
     2161 accgtgatcg gtcgcgagcc ggttgccaaa atgatgtgat cggcggtgat cgtgacgttt
     2221 ccgtcggggc cgctgacttc gatggtgttt ttgtcgcgca gcgtcgccgt accagacaca
     2281 aactgaccgc cggcgctctt aatgagctgg cgcacgcctg acgtcagttt gtcgacgatg
     2341 ctgctcttcc aatcttgcag cttgccggca tccaaagact ggccgctgac cgaaatgccc
     2401 atcgtcgaca agtgcgacag cttttcccac tgtttggcgg cgctgatcat cgctttcgac
     2461 gggatgcagc caacgttcaa acacacaccg ccgggcgcgg ccttttcgac gcaaaccact
     2521 ttcttgccga gctggccggc gcggatggcc gcgacgtagc cgccaggtcc cgagccgatg
     2581 acacaaacct cggtgcgaat gttctgcatg gtcaatcctc tgcttgagct ttgcgcggtc
     2641 cgcgtagaac cgcgaaacca gtttagtaac ggtagcggat cacgaactcg gcaaaaaaca
     2701 catctggccg cagtgtgact tgaccgaagg tggcgccgtc gcgattttcg atgccttgcg
     2761 cgggcgacag gttcagcgca ttgaacatca tcaggagcag gtcgaggttt acgccggtca
     2821 gcttcttgag cccatacgcc acctgcaagt cgaggttgaa cgtatcgggc agattgaacg
     2881 aagcccacgt cgtcggatcg ttgacccgcg ttcccgtcga gctaccgcgc ggcgaacgat
     2941 acagcgtgta cgtctgatcc tcggggctgc ggaagacttt ccactgcggc aggccggtat
     3001 agtactgaag ccgtccgcca atcgtcaggc caaagtcgaa gctgtagtcg acgagcgctt
     3061 tcaaataatg acgataggca ccagcgatcg gtccgtcgta cagcggattt agccgcgggt
     3121 tcgaaccgaa gccgttgctc tcgaagaaat cgttgacggt gccgtttagg aacgcgagcg
     3181 tatacgacgc catgatgtcc cagttgcccg gattgccgcg cacccacaga tcgacgccgt
     3241 gataggtgcg ctgggcttcg gatggggtat ccgcaacgaa aatgcgctcg cgctggccgt
     3301 ttgcatagcc gacgatccgc gtgcccgccg ggtcccaaat caggttcact tcctggttta
     3361 cccacaggtg gccgtagtga cggaacgtat agtcgatacc gaccaccgct tcgtcgagca
     3421 cctggcgatg cacacccgcg gacacttcgt cgacactcgg cggcgtcatg ttcaggtcgg
     3481 tcgcaaaacg ctggctgccc tgcccgcccg aacgccgtac ttcgtcgaac attgacgtgg
     3541 tgggattcca gcggctcagg accgatgtct gcgtcggatt ggtgtagtcg gcgatgccgg
     3601 cgttgcccag gtcgttgtgc cgtccgtagt gaaccttcag caacgtcgtg tgatcgtgca
     3661 aaaggtcata gacaaacgac agccgcggcc caaagccaag cagcgtctga atccgcgcgc
     3721 cactgacgtc gaacagatag ccggtgtcga tgcgcagacc cggcacaatc gtcagccact
     3781 tgccgacggt gtagcggtcc tgaatgaaca cgccagcgtt tatcgcctgc gatttggtca
     3841 ggtatccggc gcccgcactt ccgtcgccag gcaggctctc ggggaacgtc tccacttggt
     3901 tgcagcttcc atacgtgcgc acatcttgcg gatcgcacgg cagccccggc gtgttgtcga
     3961 ggaagcgctg cccaccggca atgccgacgc ggtggtcgta cttcatgtac gaaagctgga
     4021 agccggcttt gatgttgtgg gtgccgccga gcccgcgcgc cgtatacatc agcgtcggcg
     4081 caaactgcac attccacttg ccctgctcgt cccacggacg gttgccgttt aggtacacaa
     4141 agttgttggt cgccgcatct gccgtctgaa gaacgtgact cggagtcagc cggtccggca
     4201 ccccagcgac tttgcgcagc gtgtcttcgg agtagttttt gtacgaaacg ccggtctgta
     4261 gctgaaacaa aagcttgtcg gtgacgagcg aatcgacgag cagaccgacc cagtgtccac
     4321 cgcgctggac ccgccgctcg gcttcgggtg ccagggtgtt cggatcaatg ccgcgcagcg
     4381 ccgacgatgt cgaagcgttc aggatgtcgt taaagtcggt gctgtacgac agcgttacgc
     4441 gcgtcgcttt cgtcgcctgc cacgtcagct tgacccgccc caggtacagg atctgatcct
     4501 gatagcggtc atacgacgac ggaatctgat gccacggctg ctcggcgatc gagttcgtga
     4561 tgcggttaaa gctcgcctga aaactggtcc aaaaccacag ccggtccttg atgatcggtc
     4621 cgccgaagtt caccgccccg gaataatagg atcggtcggg aatcggcctg tcgtcgtaaa
     4681 gcagcgtcgt attttgcagc ggtgcccgcg tcggatagcg cgtcaaagcg gccataaacg
     4741 gcggctgcaa aatcgctgtc gcatcgacgg taaactggtt cccgcccgag cgggtcacca
     4801 tgttttgcac caggccgagc gagttgtatt ccgcatccat cgcaccgacg gaaatcacga
     4861 actgctcgac cgaatcagag ttgatgagcg ccgagctgga tccaaacgca ccgagggtga
     4921 tgtccgtgac ctcaagtccg tcgatgaagt agcggttgaa gcgacccagg ccgccgcgca
     4981 ccgcgacttt gtcggtgccg gacgtgacgc ccgcggtaaa ctgcgcaatc gctttttcct
     5041 gacggaacgt cggtgcgcgc gtgacctgct ggttgctcac cggggtgacg gcagcggcgc
     5101 tttcggcgtt gaggatcggg tttacttttt cgatgatctt ggtcgtctgc tgcgtcgatt
     5161 ccgcactgcc gatttccagt tcaatgtcga cgctgctgcg ctgtccggcg ttgacgcgaa
     5221 tgccgacctg tttgatcggt gcaaagccct ccatcgcgat ttgcagttcg taagtaccgg
     5281 gcggcaagtt gacgaagctg tagctgccgt cgtcgcccgt caccacctgc tgggcaccgc
     5341 cgataagctg cttgctcgtc gccgaaatcg gcacgctggc aaggccgtct ttggtgccct
     5401 tgatggtgac aaagccgccg atgctgccgc caagctcacc gacggcgccc gccgggcgaa
     5461 gcagcgaaaa aagcagtccc aagaccaaaa gaaaggaaag cggcgacgag acagcccgac
     5521 ggaaagggcg aggtttgagc atgcgcattc tcatagctag ccgagtggtg gcggcagtgt
     5581 agttt
//
LOCUS       DAORGN010000264         5564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7218_length_5564_cov_6.016700, whole genome shotgun sequence.
ACCESSION   DAORGN010000264 DAORGN010000000
VERSION     DAORGN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7218_length_5564_cov_6.016700"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKL17_19725"
     CDS             <1..384
                     /locus_tag="PKL17_19725"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNK47023.1"
                     /translation="YRWPWNVRELAKVATELKLRGAKLPQLQLDLVADRLTTDAAEPK
                     QGAAAEPVTQAARPATPQAKPAEPVVTRRDELLAVLKAHGGRIAHAARQLGKSRQQIY
                     RLLELHGLSKNDFRDDERDGNGETS"
     gene            427..1518
                     /locus_tag="PKL17_19730"
     CDS             427..1518
                     /locus_tag="PKL17_19730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNK47024.1"
                     /translation="MRNVSQTQEIVMRVLVVGPDWEDGSEVGCQNGLRAMGHEATLFD
                     PRKYIGVPRPLQRYPLAYAAAEFVLRGTVREPFFFAQKPLLETARRLRVDMVLVVQLL
                     WVLPETIAELRSQGIVCAGWFPDAFTSFGRGTFLLAPWNGLFFQDRFMVKRLRQSLSA
                     DYIHHLPECMDRVFHRPIDLTEEDRRVYGADVATFGNYYPYRAKLIEPLLDGGIDVKL
                     WGAKPPSWLHHKVHKFWAGKVVQGDEKCKAMLAAKIALNTNHYAGIGDVNKRTFELGG
                     MGAFQLTDNRPALGEYFEIGKEVVTFDGRADLREKVEYYLARPVERQQIAAAAARRAH
                     RDHTFEKRLEVLLRTVGLHPGTAPLPQGS"
     gene            1576..1968
                     /locus_tag="PKL17_19735"
     CDS             1576..1968
                     /locus_tag="PKL17_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47025.1"
                     /translation="MRRNLFAKLLYGVLPVLGLCAQTSCTPPRVSSTVYLTWKIVDAS
                     QPNPLTAPALDCNSKNVQWVRVQLAGGPSFDFPCNSYSGESTSFSSGTYSVDVIALNP
                     TGAAVSAQRVSMDLFGRTNLGNYIFQVR"
     gene            complement(1992..2978)
                     /locus_tag="PKL17_19740"
     CDS             complement(1992..2978)
                     /locus_tag="PKL17_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47026.1"
                     /translation="MKLLDTVLLFSLPASGKSEVRTYMASLTEEQCRRDFGMGPTLQL
                     DDYPYVHLMHCIDDELRAHGWRDVYYHGANRPFRDNWTWAVLIELLNEDYAHLIARKP
                     YEASSAAELLFDRLDAAHKKFGLPEVMGDVPHRIRKKVAACVESEARRELDLLNRTVS
                     QDRTGKTIVIEAARGGPNGAAFPLTPPHGYGTAFDVLSPTILERASILYIWVDPAESR
                     RKNIARGKPNAQGSILHHCVPMEVMLGQYGCDDMAYLIEQSDKPGTVRVDRIIEHDGR
                     YESRSYHLPVARFDNRVDKTTFVRVPRSEWRESDIKALHDGLSQAFAGMAVK"
     gene            3461..4681
                     /locus_tag="PKL17_19745"
     CDS             3461..4681
                     /locus_tag="PKL17_19745"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNK47027.1"
                     /translation="MPKPRLRQDVGASVLGAFVFWGLGLAWASSAAAAPANAAGSTTD
                     IAARIYKVSLRRGQTTLSERREVSEAAAAVLAQVPDGVQPSFAVIQRELLRKQVIEPV
                     HRQVLGSFATADPGTMLDSVEPVLVSALADHKWRWFGIAVAPVDAERSRLLIILVESA
                     IDLVPPPPALQVSDTPVPLSGAVLPPYDKPQVLLTDPMGQVTPLPVTIQGRKFSGQLR
                     CAMPGHYKVEVIGESKPGPHVLANFIWPCARPLESIPQVTGETRATPQVQAQTAPQTI
                     ADRESVLLKLLNRDRTEAGLPALVPDERLSHIARMHSDEMAKRHAVFHHSPTTGTPED
                     RVRRAKIKNAILAENLAQAPTEEEAERSLMDSPGHRRNILDRQLTRVGIGVATQTALH
                     GGLQLYVTQLFIGD"
     gene            complement(4682..5329)
                     /locus_tag="PKL17_19750"
     CDS             complement(4682..5329)
                     /locus_tag="PKL17_19750"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNK47028.1"
                     /translation="MRAAALLLPVALTFGCAHMAATPQSDDAARERATRQIIVQWNRQ
                     TPDPASVLRQKALPVQPGDDLTGLLAKLRQALSATGGVGIAAPQIGVSRRVFLIKHGT
                     RPTGQKVVVRAYIDPEVIARSEATQDDYEACLSVADVGGKLPRFQSLHLSYTPEDGGA
                     RQHIELFDWDARIAQHELDHLDGTLYIDRVTDPLLPISEMRRLRDEGHRARGLIK"
     gene            complement(5329..>5564)
                     /locus_tag="PKL17_19755"
     CDS             complement(5329..>5564)
                     /locus_tag="PKL17_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47029.1"
                     /translation="TFSSRNPYCPRRDPAIDQRIDYTMVGQAGALDRLQLVSSRLCFA
                     EGEAGVFPSDHFGVLTELRIATSAVTASPTEVS"
BASE COUNT         1083 a         1757 c         1711 g         1013 t
ORIGIN      
        1 taccgctggc cgtggaatgt gcgtgagctg gcaaaagtcg cgaccgagct gaagctacgt
       61 ggtgccaagc tgccgcagct tcagcttgac cttgttgccg atcgactgac caccgatgcc
      121 gcagagccaa agcagggcgc cgccgcggaa cctgtcacac aggccgcccg cccggccacg
      181 ccacaagcca aaccggcaga gcccgtggtg acccgccgcg acgagctgct ggccgtactc
      241 aaagcgcatg gcggacggat tgcccacgca gcgcgacagc ttggcaagtc gcgccagcaa
      301 atctatcggc tgctggaact gcacggccta tcgaaaaacg acttccgcga cgacgagcgc
      361 gacggaaacg gcgaaacctc gtagtacaaa aattgatcgt ccggccagct tcggggaaaa
      421 cctcgcttgc gaaacgtttc gcaaacgcag gagatcgtga tgcgggtgct ggtggttggt
      481 cccgactggg aagacggttc cgaggttggc tgccaaaatg gcctgcgcgc catgggtcat
      541 gaggccacgc tctttgatcc gcgcaagtac atcggcgtcc cgcgtccgct tcagcgctat
      601 ccgcttgcct atgccgcggc agagtttgtc ctgcgtggca ccgtccgcga gccgtttttt
      661 ttcgcgcaaa agccactgct ggaaaccgcc cgccggctgc gcgtcgacat ggtgctggtg
      721 gtccagcttc tgtgggtgct gcccgaaacg attgccgagc tgcgttcaca gggcattgtc
      781 tgtgccggct ggtttcccga tgcgtttacc agctttggcc gcggcacgtt cctgcttgcg
      841 ccttggaatg ggctcttttt ccaagaccgc ttcatggtaa agcgcctgcg gcagagcctg
      901 tccgccgact acatccatca cctgcccgag tgcatggatc gcgtgttcca ccgcccaatt
      961 gacctgaccg aagaagatcg tcgcgtctat ggggccgacg tggcgacgtt tggcaactac
     1021 tacccgtatc gcgccaagct catcgagccg ctgctagacg gtggcatcga cgtcaagctg
     1081 tggggtgcca agccgccaag ctggctgcac cacaaagtgc acaaattctg ggccggcaag
     1141 gtcgtgcaag gcgacgaaaa gtgcaaggcg atgctcgctg ccaagattgc cctgaacacc
     1201 aaccactacg ccggcatcgg cgacgtcaac aagcgaacat tcgaactggg cggcatgggc
     1261 gcttttcagc ttaccgacaa ccgccccgcg ctcggtgagt acttcgagat cggcaaagaa
     1321 gtggtgacgt tcgacggacg agccgacctg cgcgaaaaag tcgagtacta ccttgcgcgg
     1381 cccgtcgagc gacaacagat tgccgccgcc gccgcccgcc gcgcccaccg cgaccacacc
     1441 ttcgagaaac gactcgaagt gctactccgc accgttggac ttcaccccgg tacggccccc
     1501 ctgccccagg gaagctagga cgtttgtccc aaaagtcccg gcaggaaacc cccaacaaca
     1561 cattaggaga accccatgcg aagaaacctc tttgcgaagc tgctatacgg cgtgctgccc
     1621 gttctgggcc tttgcgccca gacgagctgc accccgcccc gcgtctcgtc gacggtgtat
     1681 ctgacctgga agatcgtcga tgccagccag cccaacccgc tgaccgcgcc ggcgctcgat
     1741 tgcaacagca agaacgtcca gtgggtgcgc gtccagcttg ccggcggccc cagcttcgac
     1801 ttcccctgca attcgtacag cggcgaatcc accagctttt cgtcgggcac ctattcggtg
     1861 gatgtgattg cgctaaatcc gaccggcgcc gccgtgtcgg cgcagcgggt gtcgatggat
     1921 ctgttcggtc gcaccaacct gggcaactac atcttccagg tccgctaact gcgcgaccta
     1981 aaaacggcct gctactttac ggccatgccc gcaaaggctt ggcttaggcc gtcgtgcagc
     2041 gctttgatgt cgctttcgcg ccactcggac cgcggtacgc gcacgaacgt cgtcttgtcg
     2101 actcggttgt cgaagcgcgc caccggcaaa tggtacgaac gcgactcgta tcgaccatcg
     2161 tgctcgatga tccggtcgac gcgaacggtg ccgggcttgt cgctctgctc gatgaggtac
     2221 gccatgtcgt cgcagccgta ctggcccagc atgacttcca tcgggacaca gtggtgcaga
     2281 atcgatccct gggcattggg cttgccgcgc gcgatgtttt tgcgacggct ctctgccgga
     2341 tcgacccaga tgtaaaggat gctggcgcgc tccaagatcg ttggtgaaag cacatcaaat
     2401 gccgtgccgt aaccgtgcgg cggagtcagc gggaaagcgg caccatttgg tcccccacgc
     2461 gcggcttcga tcacaatcgt cttgccggtg cggtcctggc tgacggtgcg attgagcaga
     2521 tccagctccc ggcgggcttc ggactctacg caggcggcga cttttttgcg aatgcggtgc
     2581 ggcacatcgc ccatcacttc gggaagcccg aacttcttgt gtgcggcgtc gagccggtca
     2641 aacaaaagct cggcggcgct cgacgcttca tacggcttcc gtgcaatcag gtgcgcgtag
     2701 tcttcgttga gcagctcgat cagaaccgcc cacgtccagt tgtcgcggaa gggccggttg
     2761 gcgccgtggt agtagacatc gcgccagccg tgcgcccgca gctcgtcatc aatgcagtgc
     2821 atcagatgga cgtatggata gtcatccagt tgcagcgtcg gccccattcc gaagtcgcga
     2881 cggcactgct cttcggtcag gctcgccatg taggtccgaa cttccgactt tcccgaagcc
     2941 gggagcgaaa acaaaaggac ggtatcgagc agcttcatct ggtgtgcctc cgtttgaagg
     3001 gcggcactct agcacgcggg atcgcagccg attcgcctgt ttgggggaaa ccagagcagc
     3061 aatcgttggc ccaatccggc ccggcggaac ccgtcccacg ccacaaacgc caacctgcgc
     3121 gggacaaagg aacacgccct tccgcagcaa gatccagcgg gaccgccaaa gtggcccaca
     3181 caaacgaacg gcccatgcgt ccagctccgc agcgggacca agcgtacttg ccaacacgaa
     3241 gcgatgggca gcacggcgaa cagccaggat cggcgatttt cgtccatgga tcgtttcggg
     3301 aacaacccat cgaaacggtt tcgcaagcgg gccaccgagc ggccacgtca caaaaacgcg
     3361 ccttgcggtt gtggtttcta ggaaccagca cttccggaaa tgttttttcg tcttgggccg
     3421 gcgttgcagc gcaggccgag cgacgtaaca atcggaagca atgccgaaac cgcgactgcg
     3481 ccaagatgtg ggggcatcgg tccttggggc ttttgtcttt tggggtttgg gtttggcctg
     3541 ggcttcttct gccgcggctg cgccagcaaa tgcagccggg tcgaccacag acattgcggc
     3601 ccggatttat aaagtcagcc tgcggcgtgg gcagacgacg ctatcagagc gccgcgaggt
     3661 atccgaagcc gccgctgcag tgctcgccca agttcccgat ggggttcagc cgtcgtttgc
     3721 ggtgattcag cgggaacttt tgcgcaagca ggtcatcgag cctgtgcatc ggcaggttct
     3781 cggcagcttc gcgaccgccg atccgggcac gatgctggat tcggtggagc ccgtgctcgt
     3841 cagcgcgctc gccgatcaca agtggcggtg gtttgggatc gcggttgcgc cggtcgatgc
     3901 ggaacgcagc cgcctgctga tcatcttggt cgaatcggcc attgatcttg tgccaccgcc
     3961 gcccgctctc caggtgtccg atacgccggt tccgctttcg ggggccgttt tgccgcccta
     4021 cgacaaaccg caggtgctgc tgaccgatcc aatgggccag gtgacgccgc ttcccgtgac
     4081 gattcaaggc cgcaagtttt cagggcagct gcgctgtgcg atgccaggtc actacaaagt
     4141 cgaggtcatc ggtgaaagca agccaggtcc gcacgtcctg gccaacttca tctggccctg
     4201 tgctcgccca ttggaatcca tcccccaagt gaccggcgaa acaagagcga ctccacaagt
     4261 gcaggcacag actgcgccac aaaccatcgc cgaccgggag tctgtgcttc tgaagctgct
     4321 caaccgcgat cggacagaag ccggacttcc ggcgcttgtg cccgatgaac ggctttcgca
     4381 catcgcacgg atgcacagcg atgaaatggc caagcggcac gccgtctttc atcactcgcc
     4441 aaccaccgga acgccggaag acagggtgcg tcgggcaaaa atcaaaaacg ccatcctggc
     4501 ggaaaatctc gcgcaggccc cgacggaaga agaagccgag cggagcctta tggacagtcc
     4561 agggcaccgc cgcaacatcc tcgatcgcca gctcacacgg gtgggcattg gcgtggcaac
     4621 gcaaaccgcg ctgcacgggg ggcttcagct ttacgtgacc cagcttttta tcggggattg
     4681 attacttgat caggccgcgg gcgcggtggc cttcgtcccg aaggcggcgc atctcggaaa
     4741 tcggcagcag cggatcggtc acgcggtcga tgtacaaggt gccatccagg tgatcgagct
     4801 catgctgggc aatgcgcgca tcccaatcga acagctcgat gtgctgccgg gccccgccgt
     4861 cttcgggggt atagcttagg tgcagcgact ggaaccgcgg gagcttgccg ccaacgtccg
     4921 cgacggacaa gcaggcctcg tagtcgtctt gggtggcttc ggaccgagcg atcacttccg
     4981 ggtcgatgta ggcgcgcacc accacttttt ggccggtggg tcgggtgccg tgcttgatca
     5041 aaaaaacgcg ccgagacacg ccgatctggg gggctgcaat tccaacacca cccgttgcag
     5101 aaagcgcctg ccgcagcttg gcaagcaggc cggtcaggtc gtcgcccggc tggactggca
     5161 gcgctttttg ccgcagcacg ctggcgggat cgggcgtctg gcgattccac tgcacgatga
     5221 tttggcgggt cgcacgctcc cgcgcggcgt cgtcagactg tggggtcgcg gccatatggg
     5281 cgcagccaaa cgtgagcgcg acgggcagca aaagtgcggc ggcgcgcatc aggaaacctc
     5341 ggtcggactt gcggtcacag ccgatgtggc gatacgcagc tcggtgagca ccccgaaatg
     5401 atcggacgga aacacgccgg cttccccttc ggcaaaacac agtcgcgaag aaacaagctg
     5461 aagcctgtcg agcgcgccgg cctgaccgac catggtgtag tcaattcgct gatcgatcgc
     5521 cggatcgcgg cgcggacagt acggattgcg cgacgaaaag gtct
//
LOCUS       DAORGN010000265         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7222_length_5563_cov_5.127088, whole genome shotgun sequence.
ACCESSION   DAORGN010000265 DAORGN010000000
VERSION     DAORGN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7222_length_5563_cov_5.127088"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKL17_19760"
     CDS             <1..730
                     /locus_tag="PKL17_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47030.1"
                     /translation="SQRAAARKTLHANVATLQKRLLSLCERVLAGDDGAVDSGRRVQL
                     LRLRAQLTERLYGDEPERLRALTQSSAALEKETPSDDPQGLRDRADLLRDSADKLLRE
                     MAKLKERRQALLARQRVRQRAATVDEDLFAEQATARRGGGGFSGRETKAADAAAAPTV
                     GGTPTPPTTTDPGTASGPTASLRTALDPATLDALLRSDGSGDAGAQAAALQRAEGELR
                     SLVEQLQRRATQLEQRAVTLRNQK"
     gene            complement(880..1290)
                     /locus_tag="PKL17_19765"
     CDS             complement(880..1290)
                     /locus_tag="PKL17_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNK47031.1"
                     /translation="MALITWTAEQFGTGVKKHDDEHKVLFGMLNTLHDAAAANDRAAV
                     GKALDTLIGFVVEHFASEEKNMIAAKYAAYDAHKAAHEKLVGICADLQKKFHAGQTDI
                     TTETTQFVKEWLNTHIPNVDRHYAASINAAGLGQ"
     gene            1689..2186
                     /locus_tag="PKL17_19770"
     CDS             1689..2186
                     /locus_tag="PKL17_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNK47032.1"
                     /translation="MQRPIVNLSELQFLREIAHGEKFAAKLAPVSPLIGAKKLGYNVT
                     AVPPGKRAFPLHNHHVNEELFFILQGEGTLRIGTQEHPIKSGDFICCPPGGTDVAHQI
                     VNTGSAELRYLAVSTMEPTDVFQYPDSGKFGVAANRQPGQRVLDASFGGFFREGARVD
                     YYDGE"
     gene            complement(2213..4495)
                     /locus_tag="PKL17_19775"
     CDS             complement(2213..4495)
                     /locus_tag="PKL17_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47033.1"
                     /translation="MPSIRHTRDCHKPWRLLGVMGLRFLCALVLASAALSALPSARAQ
                     SSPAAGFQVNRYEPTAAGEWGFLVDHPYYSSTRYFAGGITLNYGHNPLVRRIFSADGS
                     LLQTDAILAHQLIGHVDLAGSFLDRVTVSLSLPVTLLEQGQSLLGVTPTSPAVGDPRI
                     GAMVRVFGQPDASPISLSVGGYLWVPLRSFASSDVLPPQASDASVRGLPKLVLAGLAS
                     RIRWSASLGFLIRPSAQIGSPEIPDGATVGHEAQIGALVQYADTERRFAIGPEFLLST
                     SLSQNADQAQNYTSMELLLGAQYNIARVIQVGLAGGIGILRQPGTPDARGLLRIAYAP
                     LRKPVTDRDHDGVTDEKDICPDDHQGAHPDEQRPGCPAIDRDGDGVFDREDMCPTVSA
                     GANPDPNRRGCPLPDRDGDGVLDPADQCPDVAAGAKPDPARIGCPLADRDGDGVLDAS
                     DQCPDVAQGDHPDPKRSGCPDTDTDKDGVYDSVDRCKDVPQGPSPDRRRPGCPAGDYD
                     GDGIPDDFDACPERPGIPSSEPNKNGCPGLVSLSGGTIKLSQRVYFKHNRDTIQKVSY
                     PLLLAVATLMKAKPDIRRVEVQGHTDDRGVADYNLDLSDRRAKSVMRFLVEQGVDGSR
                     LVARGYGDTQPVASNKTVKGRTKNRRVVFVIIDPSTDGGTAPAVTAPPAAATKTEPEP
                     LPAQAPASATPAAPADAAPAQATAPTSPPVIDLTAKLAKPEDEKPVPKAPAAAKKGKQ
                     KKAGAAPAVPKPVIGEGSPP"
     gene            complement(4505..>5563)
                     /locus_tag="PKL17_19780"
     CDS             complement(4505..>5563)
                     /locus_tag="PKL17_19780"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNK47034.1"
                     /translation="APGAGGGGGFGGGGGACLSGGTGGFGGGGGGASGAAPAVGGTGG
                     YGGGSAVRSAGATGGGGMGAGGAVFVHGGTLTIVNATLTANSATGGAAGNAVMATNRG
                     NGGVGGGGAVFNLNGVVKIYNATLAANTVTGGAGQVAGAASGGAVYNLAVGTGTSATT
                     SVRNSILAGSKGGPDLFSDGILGTSSVDAQGSNIVTSSATTGTGTIAGSPISADPMLG
                     ALAANMGPAQTMALPANSPAIGAGDPAICAAAPVSGVDERGQARTCACDLGAFAVPVP
                     LPCPIPDLSGGSDDGGINDAGIDDDGGRPGNGYSQITGGGIGCSAVPNRRAAGALPIS
                     LLGIVGLVLRGLVRRRGR"
BASE COUNT         1030 a         1775 c         1721 g         1037 t
ORIGIN      
        1 gtcgcagcgg gcagccgcac gcaagaccct gcatgccaac gtggcaacgc tgcaaaaacg
       61 gctgttgtcg ctgtgcgagc gggtgttggc gggcgacgat ggcgcggtcg attcgggacg
      121 gcgggtccag cttctgcgac tgcgcgcgca gcttaccgaa aggctgtacg gcgacgaacc
      181 agagcggctg cgggcgctta cgcagtcgag tgcagcgctt gaaaaagaaa cgccgtcgga
      241 cgatccgcaa gggcttcgcg atcgggccga cctgttgcgt gattctgccg acaaactgct
      301 gcgcgagatg gccaagctaa aagagcgacg gcaagcactg ctcgcccggc agcgggtccg
      361 tcagcgtgcc gcaacggtgg acgaagacct gtttgccgaa caagcgacgg cacggcgtgg
      421 tggtggtggg ttcagtgggc gcgaaacgaa agccgccgat gccgctgcag cgccgaccgt
      481 tggtggtacg ccaaccccgc cgacgacgac agacccaggg acggcttctg gaccgacggc
      541 gtcgctgcgg accgcgcttg atccggcaac gctggatgca cttttgcgca gtgatggcag
      601 tggtgatgcc ggtgcgcagg cagcagcgct tcagcgcgcg gagggagagc tgcgcagcct
      661 ggtcgaacag ctccagcgcc gtgcgaccca gcttgaacag cgcgccgtca ccctacgcaa
      721 ccaaaagtga cgcggacagc gcggctgtat ttccgcttat ctgaacgggt tgacgctgtg
      781 ttgttgcgca gaagcgaccg gctggctggt gcagacctta cccaaggtca tatatcgaca
      841 taatcttggt ctgcacatat ggatgggact tctacacagt tattggccaa ggcccgccgc
      901 attgatggat gcagcatagt gacggtcgac gttcggaatg tgggtgttta gccattcctt
      961 cacaaactgg gtcgtttccg tcgtgatgtc cgtctgaccg gcgtggaact ttttctgcaa
     1021 atcggcgcag attccgacga gcttttcgtg cgcggccttg tgagcgtcat acgccgcgta
     1081 ctttgccgca atcatgttct tttcttcgga agcaaagtgc tcgacgacaa acccaatcag
     1141 cgtgtcgagt gcttttccga ctgcagcccg atcatttgcc gccgccgcat cgtgaagcgt
     1201 gttcagcatg ccaaacagga ctttgtgctc gtcgtcatgc ttcttcacgc cagtaccaaa
     1261 ctgctcagcg gtccaagtaa tcaacgccat gggaagctcc cttcgtgtgg tgtgaagcca
     1321 ctggcaaagt cagtggcgcg gaattcgggc tctgaccggg gccgagcggg gctgccatcg
     1381 agcagcctct cgcatccgac gaccattccg ctgcgggcca acttcccaag cagcggagcg
     1441 cgagagcacc aaatccttac gccgatgaat ctgcggcagc cgatctggtt tgtcaattcg
     1501 gtttgaaaat aacttgccca tagacgagaa acgttcaaat ggagtgattg acgtaatgtg
     1561 agatttatta tgcattgcag atacggatat tgaagtgtcc cgattgggaa cacgcaaatc
     1621 gtcccgatcg ggaacacttt ttcaatggta tgaagcccga acgcggcagc gccgccaaaa
     1681 ggagccccat gcagcgtccc atcgtcaacc tgtcggaact tcagtttctg cgcgagattg
     1741 cacacggcga aaaatttgcc gccaagctcg cgccagtcag cccgctcatc ggtgccaaaa
     1801 aactaggcta caacgtgacc gccgtgccgc cgggcaagcg ggcgttccct ctgcacaacc
     1861 accacgtcaa cgaggaactg ttctttatcc tgcaagggga aggcacgctg cgaatcggga
     1921 cgcaggaaca cccgataaag tctggcgatt tcatctgttg tccaccgggc ggcaccgacg
     1981 tagcgcacca gattgtcaac accggcagcg cggagcttcg ctacctggcg gtgagcacga
     2041 tggaaccgac ggacgtgttc cagtatcccg attcgggcaa gtttggcgtc gcggcgaacc
     2101 gccagccggg acagcgggtt ttagatgcct cgttcggtgg cttctttcgc gaaggcgcgc
     2161 gcgtcgatta ctacgacggt gaatagctgg tcatcagcgc gacggtgagc ggttacggcg
     2221 gggaaccttc gccgatgacc ggctttggca cagcaggagc ggcccccgcc tttttctgtt
     2281 tgcccttttt tgctgcagcg ggggctttgg gcaccggctt ttcgtcctcc ggcttggcaa
     2341 gcttcgccgt caggtcaatg accggcggcg aagtcggcgc tgtcgcttgg gcgggcgcag
     2401 cgtcggctgg tgcggcgggg gtcgctgatg ctggggcttg ggccggcagc ggttcaggtt
     2461 cagtttttgt cgccgctgca ggcggagccg tcaccgcagg tgccgtgccg ccgtccgttg
     2521 acgggtcgat gatgacaaac accacgcgcc ggtttttggt gcggcccttg acggtcttgt
     2581 tgctcgcgac cggctgcgta tcgccatatc cacgcgccac aagccgcgat ccatcgaccc
     2641 cttgctcgac gaggaagcgc atcacgctct tggctcgtct atcggacaga tccaagttgt
     2701 agtccgccac gccgcgatcg tcggtgtggc cttgcacttc gacgcgccga atgtccggtt
     2761 ttgctttcat cagcgtcgcg acggcgagca gcagcggata actgactttt tggatcgtgt
     2821 cgcggttgtg tttgaagtac acgcgctgcg acagcttgat ggttccgccg ctcagcgaca
     2881 caaggcctgg gcagccattt ttgtttggct cgctcgacgg aatccctgga cgttcgggac
     2941 acgcatcgaa gtcatcgggg atcccatcgc cgtcgtagtc cccggcgggg cagccgggtc
     3001 gacgtcgatc gggcgacgga ccttgcggca catctttgca gcgatccacc gagtcataga
     3061 cgccatcttt gtcggtatcg gtgtccggac agccgcttct cttcgggtca ggatgatcgc
     3121 cctgcgctac gtcggggcac tgatcgctgg catccagcac gccgtcgcca tctcgatcgg
     3181 caagcgggca gccgattcgc gccggatcgg gcttggcacc tgcggccacg tcggggcact
     3241 gatcggcggg gtccagcacg ccgtccccat cgcggtcggg cagcggacag ccacgtcggt
     3301 tcgggtcggg atttgcacct gccgatacag ttgggcacat gtcttctcga tcgaacacgc
     3361 cgtccccatc gcgatcgatt gccgggcagc cgggtcgctg ttcatcggga tgggcgcctt
     3421 ggtgatcgtc gggacagatg tctttttcgt cggtcacacc gtcgtgatca cggtccgtca
     3481 ccggcttgcg cagcggggca taagcaatcc gcaaaagccc gcgcgcgtcg ggtgttcccg
     3541 gttgtcgcag gatgccgatg cctcccgcca gtccgacttg aatcacacgc gcgatgttgt
     3601 actgcgcgcc gagcagcagc tccatgctgg tgtagttctg ggcctggtcg gcgttctggc
     3661 tcaggctggt gcttagcaaa aattcgggtc cgatggcaaa gcgccgctcg gtgtcggcat
     3721 attgcacaag cgcgccgatt tgcgcttcgt gccccaccgt cgcaccatcg ggaatctcgg
     3781 gcgagccgat ctgcgccgac ggacggatca gaaaaccgag cgaagccgac cagcgaatgc
     3841 gcgaagccag acccgcgagc accagcttgg gcaggccgcg caccgacgca tcacttgcct
     3901 gcggcggcag cacgtcactc gacgcaaagc ttcgcagcgg cacccacagg tatccgccga
     3961 cgctcagcga aatcgggctc gcatcgggct gaccaaatac ccgcaccatc gcgccaatgc
     4021 gtgggtctcc gaccgctggc gacgtcggtg tcaccccgag cagcgactgg ccttgttcga
     4081 gcagcgtcac cggcagcgac agagacacgg tcacgcggtc caaaaaggaa ccggcaagat
     4141 ccacatggcc aatcagctga tgcgccaaaa tggcgtcggt ttgcagaagc gaaccgtctg
     4201 cgctgaagat ccgtctgacg agtggattgt gtccgtagtt tagggtgata ccgcccgcaa
     4261 aatagcgcgt cgacgaatag taggggtgat cgaccaaaaa accccactct ccggcagccg
     4321 tcggttcata gcgattgacc tgaaaacccg ctgctggact gctctgcgcc cgcgcggacg
     4381 gaagcgctga cagtgcagcc gaagcgagaa ccagtgcgca cagaaaccgc agacccatca
     4441 cgcccaaaag acgccaaggt ttgtggcaat cgcgagtatg tcggatgctt ggcattcggt
     4501 tctcctatcg accccgccga cggacaagcc cgcgaagcac aagccccacg atgcccaaaa
     4561 gactgatcgg aagtgcaccg gcagctcggc gattgggaac cgcggagcag ccaatcccac
     4621 caccggtaat ctgcgagtat ccgttgcccg gacgaccgcc atcatcatcg attccggcat
     4681 cattgattcc gccatcgtcg ctgccacccg aaagatcggg aattggacac ggcagcggca
     4741 ccggcactgc gaatgccccc aggtcgcaag cgcaggtgcg ggcttgaccc cgctcgtcga
     4801 cgcctgaaac cggagccgcc gcgcaaatcg ccggatcgcc cgcgccgatt gcgggactgt
     4861 ttgccggcag cgccatcgtc tgagccggtc ccatgttggc tgccagcgcc cccagcatcg
     4921 gatcggcact gatcggcgag cccgcaattg tccctgttcc cgtcgtcgcg ctgctggtga
     4981 cgatgttgct gccttgggca tcgacgctcg acgtacccag gatgccgtcg gaaaaaaggt
     5041 ccggcccacc cttgcttccc gccaaaatgc tgttgcgcac gctggtcgtg gcgctggtcc
     5101 ccgtcccaac cgcgaggttg tagactgctc ccccgcttgc cgccccggcg acttggccag
     5161 ccccccctgt gacggtgttc gctgcaagcg tcgcgttata gattttcacg acgccgttta
     5221 ggttaaagac cgcgccgccg ccaccgacgc cgccattgcc acggtttgtg gccatgaccg
     5281 cattgcccgc ggcgccaccc gtcgcggaat tggccgtcag cgtggcattg acgatggtga
     5341 gcgttccacc atggacaaac accgcgccgc ccgcgcccat gccgccgccg cccgtcgcgc
     5401 ccgcggatcg aactgccgat ccgccgccgt agccgccggt tccgcccact gctggggcag
     5461 cccccgacgc gccaccgccg ccgccgccga atccgccggt tccacccgac aaacaagcgc
     5521 cgccgccgcc gccaaagcca ccgccaccac cggctcctgg tgc
//
LOCUS       DAORGN010000266         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7225_length_5561_cov_6.192880, whole genome shotgun sequence.
ACCESSION   DAORGN010000266 DAORGN010000000
VERSION     DAORGN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7225_length_5561_cov_6.192880"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKL17_19785"
     CDS             complement(<1..378)
                     /locus_tag="PKL17_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47035.1"
                     /translation="MHTLFRFGSLRRSRRFLPFCCSLWLSASGCAHSAKTTATATVPP
                     QTAAAPVQIKPQPPVAVIGTVPTNEEEAERVRQQLDTLSEQDETRLGRRQALIDYYCA
                     ATEQALLRGHAEDGFQLFSRALQL"
     gene            702..1529
                     /locus_tag="PKL17_19790"
     CDS             702..1529
                     /locus_tag="PKL17_19790"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNK47036.1"
                     /translation="MNVLRYLSSCVLCVGLWGCGTDNRLGRDAAATLLATTIRDYAAR
                     SADQVAKACLDLEWTLPLVGSQDVATAQTAWVRARLGYDHGAVLFKLVAPDLDALIDG
                     EVDNPLSRTGLRKMEQPLFAKPTADALTLGYAAQALAAAAVRLPPAIDDAQRTVDVGS
                     FLGALAGMSVVTGVKLDGSDSPFANQSLQSAQANLDGIAALYEPLSPLVHDVDAALDD
                     EIHGLLAGLLGQLQGVTMTEQVKDKSLFLRRSAALGQAFLRVGPVLGQSVAAVVDVT"
     gene            1547..3013
                     /locus_tag="PKL17_19795"
     CDS             1547..3013
                     /locus_tag="PKL17_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47037.1"
                     /translation="MSSCSSSFSVRQSAARCAVCLGLFVSLFSSPGLAQTAPAPTDVP
                     DPATATAPAPSSAIDLQSSVRAMPGGAQPNAAQAAPAAPTGPAIQLAPEDEDIPMQSQ
                     MVLGDAPKSAARKNGAVSLPRQSQIDVALDDRTKRMRGAAVGGYGEAILNAPIGNSQP
                     TVIDLRRTVLFFGYNFTDHIRFFSEVEYEHALTTSGSNGEVGVEQALLDFMIRRYFNV
                     RVGMGVMPVSLINIYHEPSTFNGAERPDTDLLIVPSTWQQVIAGLYGAAGAFRYQLYV
                     TPGLRADGFTADAGIRGGRQESEIRARDWGVVGRVDYAPFLGGNIGLSGYWAKAGQGD
                     PNLGDVAVTVAALDTKLSRWGFTLRGQLSYVYVSDVERLNRTLALARPGSGPVSRQLI
                     GGYVELAYDLLRLMPHNHGFQLIAFGRYERTDTQFDVIDSGLGLTRKPGNDRTVYTAG
                     LTFRPILEVALKFDYQHRYTNVSGSNTNQLNVAVAYQF"
     gene            3033..4148
                     /locus_tag="PKL17_19800"
     CDS             3033..4148
                     /locus_tag="PKL17_19800"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNK47038.1"
                     /translation="MLNTTRLPFAFLSLAGGLLFGCGNPANPVQGLSLVSIPEQVFAD
                     PPDNITTPERVELGRLLFFDPVLSENGKIACASCHHPTKGMSDGLPASPALADPKGGF
                     LTRSSPSIYDTRYQFAQFWDGRVLTMEQQALKPIENPREMGSTVQNALSRVSAIAEYR
                     DRFSAAYGELTESSLARAIAVFGRSVTANSAPVDRYLAGDTSALSKDAAAGFDLYFGK
                     ARCSFCHYVPLFNGVEGPEFEETRFRITGVPERGIVELTKDLGREEFTHDPRHRHSFK
                     TPTLRNVAHTAPYMHNGAFDTLEQVIDFYDQGAGAGQPYIVDNVDARLRGGPLKLTAL
                     EKQQLLVFLRDGLTDLSRRPDVPARVPSGLPVGSLPK"
     gene            4201..4680
                     /locus_tag="PKL17_19805"
     CDS             4201..4680
                     /locus_tag="PKL17_19805"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNK47039.1"
                     /translation="MNTQKRSFGLGLLCGLSLLVSVSQPSVAYADADRKATMELVQLV
                     TPKETYNAMIEQMTKQMLASMAQNGAKVPPDTESKMGKAVVEALPYEDLANWTVDVYS
                     TKFTTEEIKQLISFYKTPVGKKAAKLIPELSGEVGQKMGPILMQRLPAAMKKVGLTP"
     gene            complement(4763..4837)
                     /locus_tag="PKL17_19810"
     tRNA            complement(4763..4837)
                     /locus_tag="PKL17_19810"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4803..4805),aa:Cys,seq:gca)
     gene            complement(4933..>5561)
                     /locus_tag="PKL17_19815"
     CDS             complement(4933..>5561)
                     /locus_tag="PKL17_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47040.1"
                     /translation="AARRAIEDEERRKREEEEAKIRAAEDKARQEREEKERLAREEKF
                     RIDEAERRAQVEADARIEEARRIKETQERIKKQLPYKLLAGVAAAILVVAMIVIGVII
                     NKRLEEERIHQAQLAEQRKQERERAAAEERRLEEEQKKTEQAIKNLMNKLDASQKEVE
                     RKKAEEEAKKAAAAASKSSGSKKGGKGDKGKGDDGKKGGGTSVGDIDL"
BASE COUNT         1019 a         1627 c         1678 g         1237 t
ORIGIN      
        1 gagctggagc gcacgcgaaa aaagctgaaa gccatcctcg gcatgccctc gcaagagcgc
       61 ttgttccgtc gctgcgcagt agtaatcaat cagcgcttgg cgtcgtccga gccgggtttc
      121 gtcctgttcc gacaacgtgt caagctgctg gcggacgcgc tcggcctctt cttcgttcgt
      181 cgggaccgtg ccaatgacag cgacgggagg ctgtggtttt atctgcacgg gtgcggctgc
      241 tgtttgcggc ggcaccgtcg cggtggccgt cgttttggcg ctgtgggcac agccagacgc
      301 agagagccac agcgaacagc aaaacggcaa aaagcgtcgc gaccgacgca gagatccaaa
      361 gcgaaagagg gtgtgcatcg agacttttgc cagtctacta cgcggtccgt taggaaaccc
      421 aattatcgcg actgtgcttc ccaatcctgg gccgcgatgt gggccgtcac cacgtcgaga
      481 atcgctcgtt tgtgcaccgt cttggcgatg aaaccaggcg cagcttttct cctgtcgaca
      541 aggctcggtc aatcggacct ggggctcgga ctgttccatc tcgcgaaacg gtcaaagtgg
      601 ccggacggat gcgggatggc ggcatggagt cgacactgcg caagacactt gactgctgat
      661 tatggatatg attttcaatt tcacatcagg gtgcttcata catgaacgtt cttcgttacc
      721 tttcttcctg cgtgctttgt gtcggtctgt ggggctgcgg aaccgacaac cgcctggggc
      781 gcgatgcagc ggccacgctg cttgccacga ccatccgtga ttatgcggcg cgctctgcgg
      841 atcaggtggc caaggcttgt ctggacctgg aatggacgct tccgcttgtg gggtcgcagg
      901 atgtcgcgac ggcccagacg gcttgggtgc gcgcgcggct cgggtatgac catggcgcgg
      961 tgttgttcaa gctggtggcg ccggacttgg acgcgctcat cgatggtgag gtcgacaatc
     1021 cgctgtcgcg gactgggctg cgcaagatgg aacagccgct gtttgccaag ccgactgccg
     1081 acgcgctcac gcttggctac gcggcgcaag cgctggcggc ggcggcggtg cggctgcctc
     1141 ctgcaatcga tgatgcgcag cggacggtcg atgtcggatc gtttttgggg gcgctcgcgg
     1201 ggatgtcggt ggtgacgggc gtcaagctcg acggctcgga cagtccgttt gcaaaccagt
     1261 cgcttcagtc ggcccaggcc aacttggacg gcatcgccgc gctctacgag ccgctttcgc
     1321 cgctggttca cgatgtcgac gccgcgctcg acgatgaaat ccatgggctg ctcgccggac
     1381 tgcttgggca gctgcagggc gtgacgatga ccgaacaggt caaagacaaa tcgctgtttt
     1441 tgcgtcgcag tgccgcgctg ggtcaggcat ttcttcgcgt cggtccagtg ctcgggcaga
     1501 gcgtggcagc cgtggttgac gtgacctaat cgaaaagagg ttggccgtga gttcttgttc
     1561 ttcgtctttt tccgttcgtc aaagcgccgc gcggtgtgcg gtctgcttgg gtctgttcgt
     1621 ctctcttttt tcatcgccgg gcttggccca gactgcgcca gcgccgaccg atgtccccga
     1681 tcctgcaacg gcgactgcgc cggctccttc atcggcgatt gatctgcaaa gctcggtgcg
     1741 cgcgatgcca ggtggtgcgc agccaaatgc agcccaagct gcgcccgctg ccccaacggg
     1801 tccggcgata cagcttgccc ccgaagacga agacatcccg atgcagtcgc agatggtgct
     1861 cggtgatgca ccaaagagtg cggcgcgcaa aaacggtgca gtgagcctgc cgcgtcagtc
     1921 gcaaatcgac gtcgcactgg atgaccggac caaaagaatg cgcggtgccg ccgtcggtgg
     1981 ttatggcgaa gcgatcctca atgcgccgat aggaaacagc caaccaaccg tcatcgatct
     2041 gcgccgcacc gtgctgtttt ttggttacaa cttcaccgac cacattcgct tcttttccga
     2101 agtcgaatac gagcacgcgc tgaccacaag cggatcaaac ggcgaagtgg gcgtcgagca
     2161 agcgctgctg gattttatga tccggcgcta cttcaacgtc cgcgtgggca tgggagtcat
     2221 gcccgtttcg ctcatcaaca tttatcacga gccgtcgacg tttaacggcg ccgagcgacc
     2281 ggataccgac ctgctgattg tcccgagcac ctggcagcaa gtgattgccg gtctgtatgg
     2341 tgcggcaggt gcattccgtt accagcttta cgtgacgcca ggtctgcgcg ccgatggatt
     2401 cactgccgat gcgggaattc gcggcggtcg tcaggaaagc gaaattcgcg cccgtgactg
     2461 gggtgtcgtc gggcgtgtcg attatgcgcc attcctcggt ggcaacatcg gtctgtcggg
     2521 atactgggcc aaggccgggc agggggatcc aaacctcggc gatgtggcgg tcacggtggc
     2581 ggcgctcgat accaagcttt cgcgctgggg ttttacgctg cgcggacagc tctcctatgt
     2641 ctatgtaagc gatgtggaac ggctcaatcg gacgcttgca cttgcgcgtc cggggtctgg
     2701 tccggtgtcg cgtcagctca tcggtggtta cgtcgagctg gcttacgatc tgttgcgtct
     2761 tatgccgcac aatcacgggt ttcagctcat cgcgtttggt cggtacgagc ggacagatac
     2821 ccagttcgat gtcatcgatt ccggcctggg acttacacgc aaacccggaa acgatcgcac
     2881 cgtttacacg gcgggactta cgttccgtcc gatcctcgaa gtcgcgctca agtttgatta
     2941 ccaacatcgc tacaccaacg tgagcgggag caacacaaac cagctaaacg tcgcggtggc
     3001 ttaccagttc taagtggtgc ggagacaatc tgatgctgaa cacaacgcgc cttcctttcg
     3061 cattcctatc gcttgcaggc ggactccttt tcggctgtgg aaaccccgcg aatccggtgc
     3121 aaggtctgtc gctggtgtcg attccggaac aggtttttgc agatccgccc gacaacatca
     3181 caaccccaga acgggtcgag ctaggcagac tcctgttttt cgatccggtg ctttcggaaa
     3241 acggaaaaat tgcctgcgca agctgtcacc acccaaccaa aggaatgagt gacggactcc
     3301 cagccagtcc agcactggcc gaccccaaag gcggttttct gacccgatct tcgccgtcga
     3361 tttacgacac ccgctaccag tttgcgcagt tctgggatgg tcgcgtcctg acgatggaac
     3421 agcaggcgct aaagccgatc gaaaatccgc gcgaaatggg ttccacagtt caaaacgcac
     3481 tttcgcgggt gtctgcgatt gcggaatatc gcgatcgttt ctcggcagct tacggagagc
     3541 ttacagagtc ttcccttgcg cgggccatcg cggtgtttgg tcgaagcgtg accgccaaca
     3601 gtgcgcccgt cgatcgctat cttgcaggcg acacgtccgc actttccaaa gacgccgcag
     3661 cgggctttga tctgtatttt ggcaaagcgc gctgtagctt ctgccactac gttccgctgt
     3721 tcaacggcgt cgaaggacca gagtttgaag agacgcgatt ccgcatcacc ggagttcccg
     3781 agcggggtat tgtcgagctg accaaagacc tgggccgcga ggaatttacc cacgatccgc
     3841 gccaccggca ttctttcaaa acgccgacgc tacgcaacgt cgcccatacc gccccgtaca
     3901 tgcacaacgg agccttcgat acgctggaac aggtgatcga tttttatgat cagggggcgg
     3961 gcgccggaca gccgtatatc gtcgacaatg tcgatgcgcg actgcgtggt ggtccgctga
     4021 aactgactgc gcttgaaaaa cagcagctgc ttgtgttcct gcgggatgga ctcaccgatc
     4081 tgtctcgccg tccggacgtg ccggcgcggg ttccttctgg actgccggtc ggttcccttc
     4141 ccaagtagtt gctgcgattc ctgattccct tccgacttcc gcgggtgtta taagccgcgg
     4201 atgaacacac aaaaacggtc gtttggtttg ggtcttctct gcggtctttc gctgcttgtt
     4261 tctgtttcac aacccagcgt ggcgtatgcc gatgcggatc gcaaagcgac gatggagctt
     4321 gtgcagcttg tcacgccgaa agaaacctac aacgcgatga ttgagcagat gaccaagcag
     4381 atgcttgcgt cgatggcgca aaacggcgcc aaggttccac ccgacaccga aagcaagatg
     4441 ggcaaagcgg tggtcgaggc acttccctac gaagacctcg ccaactggac cgtggatgtc
     4501 tactcgacga agtttacgac cgaggagatc aaacagctca tctcgtttta caagacgccc
     4561 gtcggcaaaa aagcggcgaa gctgattccg gagctttccg gagaggtcgg tcagaagatg
     4621 ggaccgatcc tgatgcagcg gctgccggcg gcgatgaaga aagtcggtct gacgccgtaa
     4681 cccaaacgcc aagcgagctt ggcgtgggcg cgctgtactg cagaggtttc agcgcaaaaa
     4741 aaagcccctt acaagaaggg gctggaggcg acacccggat tcgaaccggg gaataaaggt
     4801 tttgcagacc tctgccttac cacttggcta tgtcgccctg cgggcttcag aaatcctgaa
     4861 gccgtgcaaa agtgagtcgg agtgtatcta tcttcgcaac tcagtcaagc agggattttg
     4921 cgacccgtgt aactacaggt cgatgtcacc gacgctcgtg ccgccgccct tcttgccatc
     4981 gtcgcccttg cccttgtcgc ccttgccgcc ttttttgctg ccagacgact tcgaagccgc
     5041 ggctgccgcc ttcttggctt cttcttcggc cttcttgcgc tcgacttctt tttggctggc
     5101 gtcgagcttg ttcatcaggt tcttgatcgc ctgctcggtc tttttctgct cttcttcgag
     5161 ccggcgttct tcggcggcag cgcgctcgcg ttcttgtttg cgctgctcgg cgagctgggc
     5221 ctggtgaatg cgctcttctt cgagtcgctt gttgatgatg acgccgatga cgatcatcgc
     5281 gacgacgaga attgcagcgg ccacaccagc gagcagctta tagggaagct gctttttgat
     5341 gcgttcctgg gtttccttga tgcggcgcgc ttcttcgatg cgggcgtcgg cttcgacctg
     5401 ggcgcggcgc tctgcttcgt cgatgcggaa cttttcttcg cgcgcgagtc gctctttctc
     5461 ctcgcgctct tgtcgagcct tgtcttccgc ggcgcgaatc tttgcttctt cctcttcgcg
     5521 cttgcgccgc tcttcatcct cgatggcgcg tcgggccgcc t
//
LOCUS       DAORGN010000267         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7310_length_5531_cov_5.247261, whole genome shotgun sequence.
ACCESSION   DAORGN010000267 DAORGN010000000
VERSION     DAORGN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7310_length_5531_cov_5.247261"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..3165)
                     /locus_tag="PKL17_19820"
     CDS             complement(7..3165)
                     /locus_tag="PKL17_19820"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNK47041.1"
                     /translation="MSAHAYSEEQLVEKPAVGLFAALGWQTVSAQDEALGDGGTLGRA
                     TRAEVVIQPRLRAALQRLNPTLPPQAIRAAIDELTRDRSSMSLEAANREVYRLLKDGI
                     SVSVPDAKHGGQTTERLRVIDWTRPEQNDFLLVRQLSVVGSLYTCRPDLVGFVNGLPW
                     VVIELKKPGVPARRAFDDNLTHYKQQIPSLFWYNALLIASNGTDSRVGSLTADWDRFF
                     EWKRIEREDEPRRVSLEVMLRGTCDRGRLLDLVEHFTLFSEHKAGLVKVLGQNHQFLG
                     VNNAIASMLAARKAGHGRGGVFWQTQGSGKSFSMVFFAQKVLRKLVGNWTFVIVTDRV
                     ELDDQLAKTFKSCGAVSDAEGDACHAKSGAHLRELLRGNHRYVFTLIHKFQTAEALTD
                     RADVIVLTDEAHRSQYDTLALNMRGAMPKATFLAFTGTPLIASEERTREVFGDYVSIY
                     DFQQSVEDGATVPLFYENRTPELELCNPDLSDDIYRLIEEAGLDEAHEAKLDKVLGQK
                     YHLLTRDDRLETVAKDIVRHFLGRGFLGKAMVVSIDKATALKMHDKVKKHWSVERARV
                     QKELARQDLSRNDRAALTRRLDVLTSTDMAVIVSPGQNEIEQMKKLGLQIEPHRRRMN
                     ESQPGLDEKFKDTQDPLRLVFVCAMWLTGFDAPSCGTVYLDKPMRNHTLMQTIARANR
                     VFPGKHSGVIVDYANVFASLEQALAIYGAGKGGARPVKDKAQLVATLHEAIADATSFC
                     AGHGVDLATLEALPLGGLERLTGFENAIDALIAPDAVRRDFFAHEKWIGTLYRAVKPD
                     PVAIECAPRVVGIATLAAAIRAKQNPNPPDISTILAQISGILDESIAGLTIRDDGPKP
                     IDLSKINFAALAQRFQQSKHKHTDLEALKAAIAAKLDRLVRLNHTRADFTEKFESLIA
                     SYNEGSRNIESLFEELLKLSNRLTEEETRHVRENLSEEELVIFDILTRPAPTLSSAER
                     AEVKKVAKELLKRLRLLLVLNWRQKSAARSALQIAIEDALDAGLPPPYTKELYQKKCA
                     ALFEHVFESYPDRNRSIYSDVA"
     gene            complement(3162..4202)
                     /locus_tag="PKL17_19825"
     CDS             complement(3162..4202)
                     /locus_tag="PKL17_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNK47042.1"
                     /translation="MLDLVPLPTDYADWLFALKVRIHTAQQRASLSVNRELIRLYWHI
                     GRDILLRQDREGWGAKVIDRLAHDLRSAFPTMKGFSRANLLYMRAFAAAWPDEEIVQQ
                     AVGQLPWGHNLVLLAKLKSAAERLAYAQRAIEHGWSRNTLTHHIERRLLAREGQAVTN
                     FAASLPRPQSDLATESLKDPYLFDFLGIGDEASERAIEDAIVQHITRFLLELGAGFAF
                     VGRQVHLEVGGEDFYLDLLFYHLKLRCFVVVELKSGPFQPEHAGKLNFYLSAVDAQLR
                     AAHDGPSIGLLLCKSRNKVVAEYALRDVSKPIGVAEYQLIEALPTDLQSSLPSIEAIE
                     QALAASSQDNPA"
     gene            complement(4206..5444)
                     /locus_tag="PKL17_19830"
     CDS             complement(4206..5444)
                     /locus_tag="PKL17_19830"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNK47043.1"
                     /translation="MRTWRHCKLGNLLEIKHGFAFLGAHFADHGTHILLTPGNFLDEG
                     GFKHKGDKEKWYQGPIPPEYILNKGDLIVAMTEQAEGLLGSCAIIPTTGLYLHNQRLG
                     LVKIHSLEQTDRKFIYYLFNSKSIRQQIRASASGAKIRHTAPTRIAEVNVYIPPLPIQ
                     KRIAGILSAYDELIENSQRRIKILESMARGLYREWFVHFRFPGHENHPKVPSPLGDIP
                     KGWEVKRLSDVADVNRLQINARSAPGRLHYVDISSVSPGQIDTITTYLFHEAPGRARR
                     VVQHGDVIWSCVRPNRRSHALVIHPDTNTIASTGFSVLTAKLIPYTFLYFSVTTDDFV
                     NYLTNNATGAAYPAVTATTFEKAKLTVPPSSLLDQFGQFTIPMSEKIHALQRQIQNLR
                     RTRDLLLPRLLSGQIDLKVD"
BASE COUNT         1063 a         1651 c         1600 g         1217 t
ORIGIN      
        1 gcccgcttac gccacgtcgg aatagatgct gcggttgcgg tcggggtagc tctcgaagac
       61 gtgctcgaac agggcggcgc acttcttttg gtacagctct ttggtgtagg gcggcggcag
      121 tccggcgtcg agcgcgtcct cgatggcgat ttgcagcgcc gagcgtgcgg cggacttttg
      181 acgccagttg aggacaagca gcagccgaag tcgcttgagc agctctttgg ccaccttttt
      241 cacctcggcc cgctctgcgc tgctcagcgt cggtgcaggg cgcgtcagga tgtcgaaaat
      301 caccagctct tcttcgctca ggttttcgcg cacatgccgg gtttcttcct cggtcagtcg
      361 attcgacagc ttcaaaagct cctcgaacag cgactcgatg ttgcggctgc cctcgttgta
      421 gctcgcgatc agcgactcga acttttcggt aaagtccgcg cgggtgtgat tcagacgcac
      481 aaggcggtcg agcttcgccg caattgccgc cttcagcgct tcgaggtcgg tgtgcttgtg
      541 cttcgactgc tgaaaccgct gcgccagcgc cgcaaagttg atcttcgaca ggtcaatcgg
      601 cttcggtccg tcgtcgcgaa tcgtcagtcc cgcaatcgac tcgtcgagaa tcccgctgat
      661 ctgcgcaagg atcgtcgaga tgtccggtgg attggggttt tgcttggccc gaatcgccgc
      721 cgccagtgtc gcgatgccga ctacccgcgg cgcacactcg atggccaccg ggtcgggctt
      781 caccgcgcga tacagcgtgc cgatccactt ttcatgcgcg aaaaagtctc gtcgtactgc
      841 atcgggagca atcagcgcgt cgatggcgtt ttcgaacccg gtcagtcgtt ccagtccgcc
      901 gagcggcagc gcttcgaggg tcgctagatc gacgccgtgg cccgcacaaa acgacgtggc
      961 atcggcgatg gcctcgtgca gcgtcgcgac aagctgcgcc ttgtctttga ccggacgcgc
     1021 gccgcctttg cccgccccgt aaatcgccag cgcctgttcc agcgacgcaa acacattcgc
     1081 atagtcgaca atcacaccgc tgtgcttgcc gggaaacacc cggttggcgc gcgcaatcgt
     1141 ctgcatcagc gtgtggttgc gcatcggctt gtccagatac accgtcccac agcttggtgc
     1201 gtcaaacccc gtcagccaca tcgcacagac gaagaccagc cgcagcgggt cttgcgtgtc
     1261 tttgaacttt tcgtcgagcc ctggttgtga ctcgttcatc cgtcgccgat gcggttcgat
     1321 ctggagcccg agctttttca tctgctcgat ctcgttttgc cccggcgaca caatcaccgc
     1381 catgtcggtc gatgtcagca catcgagccg tcgcgtcagc gccgcgcgat cgtttcgcga
     1441 caggtcttgc cgcgcaagct ctttttgcac ccgcgcccgc tcgacggacc agtgcttttt
     1501 cactttgtcg tgcatcttca gcgccgtcgc tttgtcgatg gacacgacca tcgctttgcc
     1561 caaaaagccc cgtcccagaa agtgccgcac gatgtctttg gccaccgtct ccagccggtc
     1621 gtcgcgcgtc agcaggtgat acttttgccc cagcactttg tcgagcttgg cctcgtgtgc
     1681 ctcgtcgaga cccgcttcct caatcagccg atagatgtcg tcgctcaggt caggattgca
     1741 cagctctagc tcgggcgtgc ggttttcgta aaagagcggc accgtcgcgc cgtcctcgac
     1801 cgactgctgg aagtcataaa tcgacacata gtcgccgaag acctcgcggg tccgctcctc
     1861 gctggcaatc agtggcgtgc cggtaaacgc caaaaacgtc gctttgggca tcgcaccacg
     1921 catgttgagc gccagcgtgt cgtattggct gcggtgcgcc tcgtcggtca ggacaatcac
     1981 atcggcgcgg tcggtcagcg cttcggcggt ctggaacttg tgaatcagcg taaagacata
     2041 gcgatggttg ccgcgcaaaa gctcgcgcag gtgcgcacca cttttggcgt ggcaggcgtc
     2101 gccctcggcg tcggacaccg caccacagct cttgaacgtc ttggcgagct ggtcgtctag
     2161 ctcgacgcgg tcggtgacga tgacaaacgt ccagttgccg acgagcttgc gcaggacttt
     2221 ctgcgcgaaa aagaccatcg aaaagctctt gccgctgcct tgggtctgcc aaaacacacc
     2281 gccgcgcccg tggcccgcct tccgcgccgc cagcatcgac gcaatcgcat tgttgacgcc
     2341 caaaaactgg tgattttggc ccagcacttt gaccagcccg gccttgtgct ccgaaaacag
     2401 cgtgaagtgt tcgaccaggt cgagcagccg tcctcggtcg caggtgccgc gcagcatcac
     2461 ttcgagcgac acccgacgcg gctcgtcttc tcgctcgatg cgcttccact cgaaaaagcg
     2521 gtcccagtcg gcggtcagcg acccgacccg gctgtcggtc ccgttcgacg cgatgagcag
     2581 cgcgttatac caaaacaagg acggaatctg ctgtttgtag tgggtcaggt tgtcgtcgaa
     2641 cgctcgtcgg gcgggcacgc cgggcttttt cagctcgatg accacccacg gcagaccgtt
     2701 gacgaagccg accagatcag gccgacaggt atataagctg ccgacgacgc tgagctgacg
     2761 gacgagcaag aagtcgtttt gctcgggacg tgtccagtcg atgacgcgca gcctctcggt
     2821 ggtctgtcct ccgtgcttgg cgtcgggaac cgatacgctg atgccgtctt tgagcagccg
     2881 gtagacttcg cggttcgcag cttccaggct catcgacgag cggtcgcggg ttagttcgtc
     2941 gatcgcggcc cggatcgcct gcggtggcag cgtcggattg agcctctgta gtgccgcccg
     3001 cagacgcggc tggatcacca cttcggctcg cgtcgcccgt ccgagcgtgc cgccgtcccc
     3061 cagcgcttca tcctgcgccg acaccgtctg ccagccgagc gccgcaaaca gcccgactgc
     3121 cggtttttcc acaagctgct cttcgctata ggcatgcgca ctcatgcagg attgtcctgg
     3181 ctgctcgcgg ccagcgcttg ttcgattgcc tcgatgctgg gcaggctgga ttgcaggtcc
     3241 gtcggcagcg cttcgatgag ctgatattct gccacaccga tgggcttgct cacgtcgcgc
     3301 agtgcgtact cggcgaccac tttgttgcgg ctcttgcaga gcaagaggcc gatgcttggg
     3361 ccgtcgtgcg ccgcccgaag ctgcgcatcg accgccgaca gatagaagtt cagcttgccg
     3421 gcgtgctcgg gctgaaacgg tcctgacttt agctcgacga cgacgaagca gcgcagcttc
     3481 aggtggtaaa acagcaagtc taggtagaag tcctcgcccc cgacttcgag gtgaacctgg
     3541 cgacccacaa acgcaaagcc cgcccccagc tccagcaaaa agcgcgtgat gtgctgcacg
     3601 atggcatctt cgatcgcgcg ctcgctggcc tcgtcgccga ttcccaggaa gtcgaacaga
     3661 taggggtctt tcagcgattc cgtcgccaag tccgactgtg gcctgggcag gctcgcggca
     3721 aagtttgtga ccgcctgtcc ttcccgcgca agcagccgcc gttcgatgtg atgggtcagc
     3781 gtatttcgcg accagccgtg ctcgatggcc cgctgggcat acgcaaggcg ctcggcggcg
     3841 gacttgagct tggccagcag caccaagttg tgcccccacg gcagctgtcc aacagcctgt
     3901 tggacgattt cttcatccgg ccacgccgcc gcaaaggcgc gcatgtacag caagttggcg
     3961 cgagaaaagc ccttcatcgt cggaaacgca ctgcgcaggt cgtgcgccag ccggtcgatg
     4021 actttggccc cccagccttc gcgatcctgc cgcagcagga tgtcgcgtcc gatgtgccag
     4081 tataaacgga tcagctctcg attgacggat agcgatgcgc gctgctgcgc ggtgtggatg
     4141 cgcactttga gcgcaaacag ccaatccgca taatccgtag gaagcggcac cagatccagc
     4201 atcgctcaat ccaccttcag atcaatctgc cccgaaagca gccgaggcaa aagcaaatcc
     4261 cgcgtccgcc ttagattctg aatttggcgc tgtaatgcat ggattttttc cgacattggg
     4321 atggtaaatt gaccaaactg gtccagcaga gatgagggag gaactgtcaa tttcgctttt
     4381 tcgaaggtag tcgcagtcac ggctggatat gcagcacctg tggcattgtt cgtgagataa
     4441 ttgacgaaat catccgtggt tacagaaaag tagagaaatg tatatggaat tagtttcgcg
     4501 gtaaggacgg aaaacccagt tgatgcaatt gtattagtat caggatgtat caccaatgcg
     4561 tgtgagcgtc gattgggacg aacacatgac cagatcacgt cgccatgctg aactacgcgg
     4621 cgggcgcgac ctggtgcctc gtggaacaaa taagtcgtaa tcgtgtcaat ctggccagga
     4681 ctgacagacg agatatcaac atagtgaagc cttcctggtg ccgaacgtgc attgatttgc
     4741 aatcgattca catcggcaac atcggacaat cgcttcactt cccacccctt cgggatgtcg
     4801 ccgagggggg aggggacttt ggggtggttt tcgtggccgg ggaagcggaa gtgaacgaac
     4861 cactcgcgat agagtccacg cgccatcgat tccaaaatct tgatccgccg ctggctgttt
     4921 tcaatcaatt catcataagc cgacaaaatc ccggcaatcc gcttttggat tgggagtggc
     4981 gggatataaa cattgacttc ggcaatccgg gtaggtgcgg tatgacgaat cttggcaccg
     5041 ctagctgatg cgcgtatttg ctgccggatg gattttgaat tgaatagata ataaataaac
     5101 ttccgatcgg tctgttcgag gctgtgtatc ttgacaagcc caagtcgttg attgtgaaga
     5161 taaagaccgg tggttgggat tattgcgcaa cttccaagta atccttcggc ttgctccgtc
     5221 attgcaacaa tcaagtcgcc tttatttaga atatactcgg gcggtattgg gccttgatac
     5281 cacttttctt tgtcaccctt atgcttaaat cccccttcat ctaagaagtt gcctggcgta
     5341 agaaggatat gtgttccatg atcagcgaag tgcgcaccaa gaaatgcgaa cccatgtttg
     5401 atttcaagca aattgcctaa tttacagtgg cgccatgttc tcatatcctc acgcctccaa
     5461 gatcgctgct acattggcgg cgatggtttg ttctagggcg tgggcttggg tgtttagggt
     5521 ttctagttct t
//
LOCUS       DAORGN010000268         5495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7388_length_5495_cov_5.604412, whole genome shotgun sequence.
ACCESSION   DAORGN010000268 DAORGN010000000
VERSION     DAORGN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7388_length_5495_cov_5.604412"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..818
                     /locus_tag="PKL17_19835"
     CDS             210..818
                     /locus_tag="PKL17_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47044.1"
                     /translation="MAASYFPRSDSGLVVWLQNFQSALPLQAKALGLAPAEIKDALDQ
                     SKSLAASIQVDEQKYAEWQAAVAKTAELRTQALAEIQRIIDRMKTSTGYSDEIGKTLM
                     AVPSRTESRNLDDIKPALRAQVHGGKVRIHWTRGALDGINVYSRKPGEPWTLLGTDTR
                     PPYDDTRPVPASPEVREYRAIGVFHDQEVGHPSDVVSVTVSA"
     gene            980..2188
                     /gene="proB"
                     /locus_tag="PKL17_19840"
     CDS             980..2188
                     /gene="proB"
                     /locus_tag="PKL17_19840"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNK47045.1"
                     /translation="MKTAKKKDSAAGKSRKDQLRSARRIVVKIGTGVLTQSGGKFDRA
                     RFESLCDELHRAAQTREVVVVSSGAVALGVERLGLLARPKDIRGKQAAAAVGQSRLMR
                     LYDDVLGPKGVTVAQVLLTHADIQSRTRYLNARRAIAQLIEHRALPIINENDTVSVEE
                     LKFGDNDTLAGMAVDLVEAELLIILTDIDGLYTADPRKDPKATRIPEVFGIDPATLGL
                     AGDSTSGVGTGGMATKLRAASRAGESGVPTLIAPGKQVGVLDAALAGEPLGTFVHAPP
                     QPETGSRKRWLLRDLRPTGQLVIDDGADRALCERGKSLLPSGITEVRGTFEAGEPVEI
                     LSTDAVAVARGFSSYGSDELRRICGLKTADIVEKLGYKLADEAVHRDDLVLLETLRKR
                     PSKKSKGESA"
     gene            2185..3492
                     /locus_tag="PKL17_19845"
     CDS             2185..3492
                     /locus_tag="PKL17_19845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNK47046.1"
                     /translation="MSQTVDVAGDQLDVARGLALLAQRGSEPLRSVSSEKKNAALSKL
                     AALLTDPSRMQVVLQANAQDIEAGHKAGLSAALLDRLRLDEGRLHALAKAVDEIAALP
                     DPVGEVVESWSRPKGFVVEKVRIPLGVLLLIYEARPNVTVDAAALALKSGNAVILRGG
                     KEALSSNRALAALVREALSSQGLPAEACQFVDNPDRGLLLALLSQSGLIDLCIPRGGE
                     ALIRFVVEHARIPVVQHYQGICHLFVDESADLTMATELVYNGKCSRPGVCNALECLLI
                     HERVAAPLLLRLCERLAPAGVELRLEPDALALLPSPLPKGLRVQAAQPSDFGHEFLDL
                     TLAVRIVPSLDAAISHIARYGSLHTEVICTQNEEHAQRFVREVCASFVGWNVSTRFND
                     GGELGLGAEVGISTTKLHAFGPMGLRELCATKFVVKGTGQVRT"
     gene            3580..4776
                     /locus_tag="PKL17_19850"
     CDS             3580..4776
                     /locus_tag="PKL17_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47047.1"
                     /translation="MKALRTVFERCVTRRFSPLTLALSAVMLLPAPAFAKADRPAKAD
                     KAASKTAAVAKGPSKPEAAALPDAFSQTPETSRSFEDLLKDAVPVKDFATLIEPLYAR
                     CDDPDPLYKRQCEVSKTFLLDYLSSHTFVAEADMPPDTSPYDAAAKQVDMEISGCLVC
                     KNPITVAGEPRYLTTKPPQRVQDGRAVVEPLANHEIAIEDRTRADRFVEKVVPRLKVQ
                     HVFRFATPYPQELAQAAVGKPAAPIPPPAKGVLVASLGHRVYDRCTGQVAAASPKAAT
                     AVKVTPDRSCPRNAADDMSQAEIKKQQEILALPEQLSPREIDRVLAPIQARVHECYVE
                     FGEPSGTAKVSLVIGPEGKLTQVQLPSPFDKADIAVCLRSQLKSAVFPKFRGSPMKFE
                     YVYQVQ"
     gene            4862..>5495
                     /locus_tag="PKL17_19855"
     CDS             4862..>5495
                     /locus_tag="PKL17_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47048.1"
                     /translation="MTLLPFVLPACSKGGAGDPTSCRKLSDPVTMGSTDRPIGRSLYV
                     ELKTPSGAFVTVPLEGIGGEYGGTKQMGKCDERGQYTVERVVLVNAKQELVAVANRLP
                     GSASFKIDYSDGRTTSVAGTSVANYYSEYGTAVASGAVTLQTISASSKLAKQGDRVTV
                     SVSLQGDECGLLKSQWWLADSARKQVTTASNVAGGTGTVMIPVPVDLTPQT"
BASE COUNT         1053 a         1582 c         1881 g          979 t
ORIGIN      
        1 gcgggtgcgc atggcgtgac gaggggtgcg catggcgtga cggcgggtgc gcatgtgtcg
       61 cgcgggcgtc ggcagatcat tttttttcaa aaaaacgaca tccggggtgg cagcgcggct
      121 aggtgtgagg caacctctca aagtttgcgg tgtcgcatgg tgtggcactt tgcagacatc
      181 tgacggtccg tttttggagg atgtgagaga tggcagccag ttattttccg cgcagtgact
      241 cggggcttgt tgtgtggctc cagaactttc aaagtgcact gcctttgcag gccaaagcgc
      301 tcgggcttgc gcctgccgaa atcaaagacg cgctcgacca gtccaagtcg ctggcggcgt
      361 cgattcaggt ggacgagcaa aagtacgctg agtggcaggc cgcggtcgcc aaaacggcgg
      421 aacttcgcac ccaggcgctc gccgaaatcc agcgaatcat cgaccggatg aagacatcga
      481 ctgggtacag cgacgaaatc ggcaagacgc tgatggcggt gccgtcgcgg accgaaagtc
      541 ggaatttgga cgacatcaaa ccggcgctgc gggcccaggt ccacggtggc aaagtgcgga
      601 ttcactggac gcgcggcgcg ctcgacggaa tcaacgtgta cagccgaaag cctggcgagc
      661 cgtggacgct gctgggcacc gacaccaggc ctccttatga cgacactcgc ccggttccgg
      721 cgtcgccgga ggttcgcgaa taccgcgcga tcggcgtgtt tcacgaccag gaagttggcc
      781 acccaagcga tgtcgtcagc gtgacggtca gcgcgtagcc gtcaacggga aagaagccgg
      841 cctgcccacc cggcgcagcg gaacaaagag cggctggctg gccgtggggg gtggcacacg
      901 gcgcgccaat tcgccaagat ggcggggccg cgattcggca cgccatgcgg gcggcgtttg
      961 ggtccggcgc ttttcagcca tgaagacagc gaaaaagaaa gacagcgcgg cgggcaaaag
     1021 ccgcaaagat cagcttcgat cggctcggcg tatcgtcgtc aaaattggca ccggggtgct
     1081 gacgcagagc ggcggcaagt tcgatcgggc gcgcttcgag agcctgtgtg acgagctgca
     1141 ccgggccgcc cagacccgcg aagtggtggt ggtgtcgtcg ggggcggtgg cgcttggcgt
     1201 cgaacggctc gggcttttgg cgcgacccaa agacattcgc ggcaagcagg cggcggcggc
     1261 agtcgggcag tcacgactga tgcggcttta tgacgatgtg ctcgggccca aaggcgtgac
     1321 ggtggcgcag gtgctgctga cgcacgccga catccaatcg cggacccgtt atctgaatgc
     1381 gcgacgggcg attgcgcagc ttatcgaaca ccgagcgctg cccatcatca acgaaaacga
     1441 cacggtcagc gtcgaagaac tgaagttcgg cgacaacgac acgctggccg gcatggcggt
     1501 cgatctcgtc gaggcggagc tgctcatcat cctgacggac atcgacggac tttatacggc
     1561 ggacccgcgc aaagatccca aagcgacgcg gattcccgag gtgttcggca tcgatccggc
     1621 gacgctgggg ctggcggggg acagtacgtc gggcgttgga acgggcggga tggcgacgaa
     1681 gctgcgggcg gcgagccggg ctggggaatc tggtgtgccg acgctgattg caccgggaaa
     1741 acaagtcggg gtgctggatg cagcgctcgc cggggaaccg ctgggaacgt ttgtgcatgc
     1801 gccgccacaa cccgagacgg ggagccgcaa gcgctggctt ttgcgcgatc tgcgcccgac
     1861 gggccaactg gtcatcgacg acggggccga ccgggcgctg tgtgagcgcg gcaagagcct
     1921 tctaccaagc ggcatcaccg aagtgcgcgg gacatttgaa gccggcgaac cggtcgagat
     1981 tttgtcgacg gatgcagtgg ccgtggcgcg cggtttttcc agctacggat cggatgagct
     2041 gcgacggatc tgtgggctga agacggcgga cattgtcgaa aagctcggct acaagctggc
     2101 cgacgaggcc gtccatcgcg acgatctggt tctgctggag actttgcgca agcgcccaag
     2161 caaaaagagc aaaggagaga gcgcgtgagc cagacggtgg atgtggcagg tgatcagctc
     2221 gacgtagcgc ggggactggc gcttttggcg cagcgcgggt cggagcctct gcgcagtgtg
     2281 tcttcggaaa agaaaaatgc ggcgctcagc aagctggcgg cgcttttgac cgatccgtcg
     2341 cggatgcagg tggttttgca agccaatgcg caggacatcg aggcgggaca caaggctggg
     2401 ctttcggcgg cactgctcga tcggctgcgg ctcgacgaag ggcgactgca cgcactggcc
     2461 aaagcggtgg acgaaattgc ggcgctgccc gatcccgtcg gggaagtcgt cgagtcgtgg
     2521 tcgcgcccga agggttttgt ggtcgaaaaa gtacggatac cgctcggggt gctgctgctg
     2581 atttacgaag cgcgaccgaa cgtgaccgtc gatgcagcgg cgctggctct aaaaagtggc
     2641 aatgcggtga ttctgcgcgg tggcaaagaa gcgctgtcgt cgaatcgggc gctggccgcg
     2701 ctggtccggg aagcgctctc gtcacaaggt ctgcctgccg aagcctgtca gtttgtcgac
     2761 aatcccgacc gcgggcttct gctggcgctg ctttcgcaga gcgggcttat cgacctgtgc
     2821 attccgcgcg gtggcgaagc gctgattcgc tttgtcgtcg aacatgcccg gattccggtg
     2881 gtccagcact atcaggggat ctgtcacctg ttcgtcgatg agtcggcgga cctgacgatg
     2941 gcgacggagc ttgtctataa cggcaagtgc tcgcgaccgg gtgtgtgcaa tgcgctggag
     3001 tgtcttttga tccacgagcg ggtggcggcg ccgcttcttc tgcggctgtg cgagcgtttg
     3061 gccccggcgg gcgtcgagct gcggcttgaa ccagacgcgc tggcgctttt gccgtcgccc
     3121 ctgccgaaag gtctgcgggt ccaagcggcg cagccgagcg actttggcca cgaatttttg
     3181 gacctgacgc tggcggtgcg gattgtgccg tcgctcgatg cggcgatttc ccacattgcg
     3241 cgctatggat cgctgcacac cgaagtgatc tgcacgcaga acgaagaaca tgcgcagcgc
     3301 ttcgtccgcg aggtctgcgc gagctttgtc ggctggaatg tatcgacgcg gttcaacgac
     3361 ggcggcgagc tgggactggg cgcagaagtc ggcatctcga cgaccaaact gcacgccttt
     3421 ggcccaatgg gtctgcgtga gctgtgcgcc accaagttcg tggtcaaagg aacggggcag
     3481 gtgcgcacct gacgcgccgc gcggtttgtc cgttcgtcgc tggtcttgat gtagcaaagc
     3541 cgcttacacg accccggaaa tcgtgttatg caggcaaaaa tgaaagcctt gcgaaccgtg
     3601 ttcgagcgtt gtgtgacgcg gcgtttttcg cctctgactt tggcactctc ggcggtgatg
     3661 cttttgcccg cgccggcctt tgcgaaagcc gataggcccg ccaaagccga caaagcggcc
     3721 agcaagaccg cggctgtggc caaaggccca agcaaacccg aagccgcagc gctgccggat
     3781 gcgttttcgc agacgcccga gacgagccgc agcttcgagg atttgctaaa agacgcggtg
     3841 ccggtcaaag actttgcgac gctcatcgag ccgctgtatg cgcgctgcga tgatcccgat
     3901 ccgctctaca aacggcagtg cgaagtgtcc aagacgttcc ttttggacta tttgagcagc
     3961 cataccttcg tcgctgaagc cgacatgccg ccggatactt cgccgtatga cgctgccgcc
     4021 aagcaggtcg acatggagat ttccggctgt ctggtctgca aaaacccaat caccgttgct
     4081 ggcgagccgc gctatctgac gaccaagccg ccgcagcggg tgcaggatgg acgtgcagtc
     4141 gtcgaaccgc tggcgaacca cgaaattgcg atcgaagacc gcacccgcgc cgatcgtttc
     4201 gttgaaaaag tggtgccgcg tctgaaagtg cagcatgtgt tccgctttgc gacgccgtat
     4261 ccgcaagagc ttgcacaagc ggcggtggga aaaccggcag cgccgattcc gccgccggcc
     4321 aagggcgtgc tcgtggcgtc gctggggcat cgcgtctatg accgctgcac cggacaagtt
     4381 gcggcggcgt cgccgaaagc ggccactgcg gtcaaggtca cgccggacag gagctgtccg
     4441 cgcaacgctg ccgacgacat gtcgcaggcc gagatcaaaa aacagcaaga aatcctggcg
     4501 ctgcccgaac agctttcccc gcgagaaatt gaccgggtgc tggccccgat tcaggcccgc
     4561 gtgcacgaat gctacgtcga gttcggcgag ccgtcgggca ccgccaaagt cagcctggtc
     4621 atcggtccgg aaggcaagct gacgcaagtg cagctgccgt cgccatttga caaagcggac
     4681 atcgcggtgt gcctgcgctc gcagctgaaa agcgcggtgt tcccgaagtt ccgtggcagt
     4741 ccgatgaagt tcgaatacgt ctatcaagtg cagtagtcgt cgcgctttca cccttcccca
     4801 aaggcaaaac atgctgcccg ttcgtttccg tcgctccgtc tgccaagggt ggacgctctg
     4861 tgtgaccctg cttccttttg tgctgccggc ttgtagcaaa ggtggcgccg gcgatcccac
     4921 gtcgtgtcgc aagctgagcg atccggtgac gatgggaagc acggaccggc ccatcgggcg
     4981 gtcgctttac gtcgagctga agacgcccag tggcgcgttt gtgacggtgc cgctcgaagg
     5041 catcggcggt gagtacggcg gcaccaagca gatgggcaag tgcgacgagc gcggccagta
     5101 caccgtcgag cgcgtggtgc tggtgaacgc caaacaagag ctggttgccg tcgccaaccg
     5161 actgcccgga tcagccagct tcaagattga ttattccgac gggcgcacca caagcgtcgc
     5221 cggaaccagc gtcgccaact actacagcga gtacggcacc gcggtggcaa gtggcgcggt
     5281 gacgcttcag acgatttcag cgagcagcaa actggcgaag cagggagatc gggtgacggt
     5341 ttccgtcagc ctgcaaggcg atgagtgcgg tctgctcaag agccagtggt ggctggctga
     5401 ctcggcgcgt aagcaggtca cgacggcgtc taatgtcgcc gggggaacgg gcaccgtcat
     5461 gatcccggtg ccggtggatt tgacgccgca gactt
//
LOCUS       DAORGN010000269         5481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7425_length_5481_cov_5.655547, whole genome shotgun sequence.
ACCESSION   DAORGN010000269 DAORGN010000000
VERSION     DAORGN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_5481_cov_5.655547"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKL17_19860"
     CDS             complement(<1..427)
                     /locus_tag="PKL17_19860"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNK47049.1"
                     /translation="MTRSPDSLWQRAVRFAKSILTGGAASLADFATLTALVELFRLTP
                     TQANIPSLLVGATIQFVGNRHLVFSAGHLAMGPQLFGFVLVEMGTFLLNAGSFYVVVR
                     LSPIPYPIVRLVCSFLVFSLFSYPLWKRVFADKQNGTKKD"
     gene            529..1614
                     /locus_tag="PKL17_19865"
     CDS             529..1614
                     /locus_tag="PKL17_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNK47050.1"
                     /translation="MGGSAAPTAPPSVDETLSAVAEIKDARQAILREIEKRIIGQRHV
                     IDQLLVALFARGHCLFVGVPGLAKTLLISTLSEVLSLRFSRIQFTPDLMPSDITGSDI
                     LEEDQTTGKRVFRFIRGPIFANIVLADEINRTPPKTQAALLQSMQEGRVTAGGQTHEL
                     TPPFLVFATQNPIEQEGTYPLPEAQLDRFMLQVDVGYPTLEEEVQIVKRGTSADKPVI
                     QKILSPERIMQLQDLTLRVPAADHVVRYAVSLCHATRPQRVPGARANRTSGSIRLAGG
                     PKEAEALSLVSEYLAYGAGPRASQFLILGAKAKAILEGRPAASIDDVRALARPVLQHR
                     LIVNFHAEAEGIGPQVIIDKLLEAIRP"
     gene            1627..2586
                     /locus_tag="PKL17_19870"
     CDS             1627..2586
                     /locus_tag="PKL17_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNK47051.1"
                     /translation="MNSRLLDLPSLLRLQSLLLRARVVADGALQGLHRAAHHGSSVEF
                     AEHKEYAPGDELRHIDWRAVGRMDKYYVKRFEEEAEVRAYVVVDASGSMGYGRQDQLT
                     KLDFAKLLAAALSYLLLRQHDPAGLLIAKDEGPLYIPPRGGAAHLGDLCRALEDTPAQ
                     GRTELSDALLLLSETVRRRSIIVVLSDLLPAPPLDDVDEYAEYLRKQSVLLRAKLCSL
                     RAQKHDVIVLQTLDADELELPWSNLTWFEEVEPGLPSQERLFVDPGDVRKAYLDELQQ
                     FLNDMRMGLREGDVDYTLVPTSRSPESVLLDMLRGPLRQKVRR"
     gene            2590..4797
                     /locus_tag="PKL17_19875"
     CDS             2590..4797
                     /locus_tag="PKL17_19875"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNK47052.1"
                     /translation="MHLLSPIYAAFLFLGILPILIHLHGRRRAQVRKLPTLLLLRASS
                     RRVAQRTRLRHVLLLLLRVLLLVAIPFLLARPFAETDSDLPVQVSQTQRAVLIFDDSL
                     SMTYKPGPDQKLGSAMLLERGRKLATRIVDALPQGAEAALILGSRGGDAPVGELTSDR
                     TRLYSAISALRPTYRPTDLQSAFKRATQILSTQRGTLRRIYVISDSSAHALDASLHAP
                     AETEVSLVDVRDGQPLPNCAVTDLRAEPAASMGPRAIRVMAELSNFGAEPVKELQVTL
                     LVDGQAVAKGLVDLSPRGQAVKRFIHILPPEKETRSAQSPPESQTTQSGGLHHLAVRI
                     APDALEADNERHLRVDVQAVLRVLILDGDPRNLRRDDEAYYVETALRPGERDDSPLQL
                     TTRTIDEGLGTLADFDAVVLLNAKAQELSRRGIERGLLDYVQRGGGLLIGLGDNVEVD
                     AYNRVLAELLPQPLAVVKTAGALPASGSGEVESSTQGPGEHIGQLDRRHPLLQPFLSG
                     RSSESLLSASFARYLLLLPTPRSQGQTTVLLSFESGAPALVERSIGSGRVMLFTSTLD
                     RDWNDLAIQPAFLPLLQQMVRHLSRAPLQKAETETLIGQTRDVRLQPGDSRVEVTLPS
                     GKTRLHERLSGRKLLQFAETREPGFYRVATAGDTGILRPRPTEHFVVNVDAAESDLQL
                     APASRLAALQRPLITSDTSPGGKNPKRRVELWHAMGWMLLLILLGESLLLRQK"
     gene            4827..>5481
                     /locus_tag="PKL17_19880"
     CDS             4827..>5481
                     /locus_tag="PKL17_19880"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNK47053.1"
                     /translation="MSRYVVAIDQGTTGSTVLLLSETLVVVSRGYAEFAQIYPQPGWV
                     EHDPETIWQSVLTALGQALSSAHIDPREIAAIGITNQRETTVCLSRDGKPLHNAIVWQ
                     DRRTAARCAELRKAGHLAEVQKKTGLVLDPYFSGTKIEWLLSQVPGLLVRASSGDARF
                     GTIDSFLVHRLSGGEAWVTDPSNASRTLLFNIESNRWDDSLCALLGVPVGCLPRVVAS
                     "
BASE COUNT          982 a         1771 c         1708 g         1020 t
ORIGIN      
        1 cgtctttttt tgtcccgttc tgcttgtcgg caaacacccg tttccacagc ggatagctaa
       61 acagcgaaaa caccaggaac gaacagacga gccgcacgat cggataggga atcggcgaaa
      121 gccgcaccac gacgtaaaag ctgccggcgt tgagcagaaa cgtccccatc tcgacgagta
      181 caaagccaaa aagctgcggc cccatcgcca ggtgtcccgc cgaaaacacc aggtggcggt
      241 tgccgacaaa ctgaatcgtc gcaccgacca aaagcgacgg aatgttcgct tgggttgggg
      301 tcagccggaa cagctcgacc agcgcggtca gcgtcgcaaa gtccgcaagc gatgccgccc
      361 cgccagtcaa gatcgacttc gcgaagcgca ctgcgcgctg ccatagggaa tccggagaac
      421 gtgtcaccgc tcgatcgata tacccgatcg ggcgtgagcc gggcggccaa actgtgtcac
      481 aatgcgcctg tgactgctca atcagatgtg cctgcggatg ccgatggcgt gggtggaagc
      541 gcggccccga cggctcctcc ttctgtcgac gaaacgctgt ctgcggtggc cgaaatcaaa
      601 gacgcccggc aagcgatcct gcgcgagatc gaaaagcgca tcatcggtca gcgccacgtc
      661 atcgaccagc tcctcgtcgc gctgtttgcc cgtggccact gtctgtttgt cggtgtgccg
      721 ggactggcca agacgctgct tatttcgacg ctgtccgaag tcctgtcgct gcgcttttcg
      781 cgcatccagt ttacgccgga cctgatgccg tcggacatca ccggcagcga catcctcgaa
      841 gaagaccaga ccaccggcaa gcgcgtcttc cgttttatcc gcggtccgat tttcgcaaac
      901 atcgtcctcg ccgacgaaat caaccgcacg ccgcccaaga cccaggccgc gctgctccag
      961 tcgatgcagg aaggtcgtgt caccgcgggt ggtcagaccc acgagctgac cccgccattt
     1021 ttggtgtttg cgacgcaaaa tccgatcgaa caggaaggca cgtatccact tcccgaagcg
     1081 cagctcgacc gcttcatgct gcaagtcgat gtcggctatc cgacgctgga agaagaagtc
     1141 cagatcgtca agcgcggcac gtcagcggac aagccggtga tccaaaaaat cctgtcgccc
     1201 gagcggatca tgcagcttca agacctgacg ctgcgcgttc ccgccgccga ccatgtggtg
     1261 cgttacgccg tctctctctg tcatgccacc cggccccagc gcgtccccgg tgcccgcgcc
     1321 aatcgcacaa gcggcagcat ccgtctggcc ggcggaccca aagaagccga agcgctgtct
     1381 ttggtcagcg agtatttggc ctatggtgcc gggccgcgtg catcgcagtt tttgatcctg
     1441 ggcgccaaag ccaaagcgat tctggaaggc cgtcccgccg cgtcgatcga cgacgtgcga
     1501 gcgctggccc gtccggtgct tcagcatcgc ctgatcgtaa acttccacgc agaggccgaa
     1561 ggcatcgggc cgcaggtcat catcgacaag ctcctcgaag ccatcaggcc ctagtccgtt
     1621 tcgtccatga actcgcggct gctcgatctt ccgtctctgc tacgccttca gagcctgctt
     1681 ttgcgggcac gggtggttgc ggacggcgct ttgcagggtc tgcatcgggc ggcgcaccac
     1741 ggctcgtccg tcgagtttgc cgagcacaaa gagtatgccc ccggcgacga gctgcgccac
     1801 atcgactggc gtgccgtcgg acggatggac aaatactacg tcaagcgctt cgaggaagaa
     1861 gccgaagtcc gcgcctatgt cgtcgtcgat gcgtccggat cgatgggcta cggacggcaa
     1921 gatcagctga ccaagctcga ctttgccaag ctgctcgcgg cggcgctgtc gtatctgctt
     1981 ttgcgtcagc acgatcccgc cgggctgctc atcgccaagg acgaggggcc gctgtacatc
     2041 ccgccgcgcg gtggggcggc gcacctgggg gacttgtgcc gcgcactgga agacacgccg
     2101 gcgcagggtc gcaccgagct ttcggacgcg ctgctgctgc tatcggaaac cgtgcgtcgc
     2161 cggtcgatca tcgtggtgct gtcggatctg ttgccggcgc cgccgctcga tgatgtcgat
     2221 gaatacgccg aatacctgcg caaacagtcg gtgcttttgc gcgccaagct gtgcagtctg
     2281 cgcgcccaaa aacacgatgt cattgtcctg caaacgctcg atgccgacga actggagctg
     2341 ccgtggtcga acctgacgtg gtttgaagaa gtcgagccgg ggctgccttc gcaagagcgg
     2401 ctttttgtcg atccgggcga cgtgcgcaaa gcctatctcg acgagcttca gcagtttctc
     2461 aatgacatgc ggatgggtct gcgggaaggt gacgtcgact acacgctggt gccgacatcg
     2521 cgttcgccag aaagtgtgct gctcgacatg ctgcgcggcc cgctgcgcca gaaggtgcgg
     2581 aggtaagccg tgcatcttct gtcgccgatt tacgcggcct ttctgttttt gggcatcttg
     2641 cccatcctga ttcacctgca cggacggcgg cgggcccagg tgcgcaagct gccgacgctg
     2701 ctgcttttgc gggcgtcgag ccgtcgggtc gcccagcgca cccggctgcg gcatgtgctg
     2761 cttctgcttt tgcgcgtgct gctgcttgtc gcgatcccgt ttttgctcgc caggccgttt
     2821 gccgaaaccg attcggatct gccggtgcaa gtcagccaga cccagcgcgc ggtgctgatc
     2881 ttcgacgaca gcttgtcgat gacgtacaag ccgggaccgg accaaaagct cggatcggcg
     2941 atgctgctgg agcgcggacg caagctggcc acccgaatcg tcgatgcgct gccgcaaggg
     3001 gcggaagcgg cgcttatctt gggttcgcgt ggcggcgatg cgccagtcgg cgagctgaca
     3061 tcggaccgga cgcggcttta ttcggcgatt tcggcgctaa ggcccaccta tcggcccacc
     3121 gatctgcaaa gcgcgtttaa gcgagcgacg cagattctgt cgacccagcg cggcacgctg
     3181 cggcgcattt acgtgataag cgacagctcg gcgcacgcgc tcgacgcctc gctgcatgcg
     3241 ccggcagaaa ccgaggtgtc gctcgtcgat gttcgcgatg gccagccgct tcccaactgt
     3301 gccgtgaccg acctgcgcgc cgagcctgcc gccagcatgg gaccgcgcgc cattcgggtg
     3361 atggcggaac ttagcaactt tggcgccgag cccgtcaaag aacttcaggt cacgctgctc
     3421 gtcgatgggc aagccgtggc caaggggctt gtcgacctgt cgccacgcgg tcaggcggtc
     3481 aagcgcttca tccacatcct gccgcccgaa aaagagaccc gatcggccca gtcgccgccg
     3541 gaaagtcaga ccacgcagag cggcggcctg caccacctgg cggtgcgcat tgcccccgac
     3601 gccttggaag ctgacaacga acgccacctg cgcgtcgatg tgcaggcggt gctgcgggtg
     3661 ctgatcctgg acggcgaccc gcgcaacctc cgccgcgatg acgaagccta ctatgtcgaa
     3721 acggcgctgc gccccggcga acgggatgac tcgccgcttc agcttaccac ccgcaccatc
     3781 gacgaaggac tggggacgct tgctgacttt gatgcggtgg tgctgctcaa cgccaaggcg
     3841 caagagctgt cgcggcgtgg catcgagcgc ggacttttgg actatgtgca gcgcggcggc
     3901 ggacttctga ttgggcttgg cgacaatgtg gaagtcgacg cctacaaccg ggtgctcgcc
     3961 gagcttttgc cgcagccgct tgcggtggtc aagacggcgg gggctctgcc agcaagtggc
     4021 agcggggaag tcgagtcatc gacgcagggt ccaggcgagc acatcggcca gctcgaccgt
     4081 cggcacccgc tgctacagcc gtttctgtcc gggcgttcga gtgagtcgct gctgtcggcg
     4141 tcgtttgccc gctacctttt gcttttgccg accccgcgca gccagggcca gaccacggtg
     4201 cttttgtcgt tcgagagcgg cgcgccggcc ttggtcgagc gcagcatcgg gtcgggccgc
     4261 gtcatgctgt ttacctcgac gctcgatagg gactggaatg atctggcgat tcagccggcc
     4321 tttttgcccc tgctgcagca gatggttcgc catctgtcac gggcgccgct tcagaaagcc
     4381 gaaaccgaga cgctgattgg ccagacccgc gacgtgcggc ttcagcctgg tgactcgcgc
     4441 gttgaagtga cactgccatc gggcaagacc cggctgcacg aaagactgtc gggccgcaag
     4501 cttctgcagt ttgccgaaac ccgcgaaccc ggcttttacc gcgtcgccac cgccggggac
     4561 accggcatcc tcaggccgcg gcccaccgaa cactttgtcg tcaacgtcga tgcggctgaa
     4621 tccgaccttc agcttgcgcc ggcctcgcgc ctggctgcgc tgcaaagacc gctgattaca
     4681 tcggacacaa gccctggcgg aaaaaatccc aagcggcggg tcgagctgtg gcatgccatg
     4741 gggtggatgc ttctgctgat cctgctcggc gagtcgctgc tgttgcgcca gaaataacca
     4801 agaccacacc gaaaggaact tcgcccatgt cgcgctacgt cgttgccatc gatcaaggga
     4861 ccaccggctc gaccgtgctc ttgcttagcg agacgctggt tgtcgtcagc cgcggctacg
     4921 ccgagttcgc gcagatctac ccgcagcccg gctgggtcga acatgatcca gagacgattt
     4981 ggcagtcggt cttgacggca cttggtcagg cgctttcgtc ggcccacatc gacccacgcg
     5041 agattgcggc catcggcatc accaaccagc gcgaaaccac cgtctgcctt tcgcgcgatg
     5101 gaaaaccgct gcacaacgcg attgtctggc aggaccgccg caccgccgcc cgctgcgccg
     5161 agctgcgcaa ggcgggccac ctggccgaag tgcaaaaaaa gaccggcctt gtgctcgacc
     5221 cctacttttc ggggaccaag atcgagtggc tgctttcgca agtgccgggg cttttggttc
     5281 gggcttcttc gggcgatgcc cggtttggca ccatcgacag cttccttgtc caccgcttgt
     5341 ccggcggcga ggcgtgggtg acggacccct cgaatgcatc gcggacgctg ctcttcaaca
     5401 ttgaatcaaa ccgctgggac gacagcctgt gcgcgctctt gggggttccc gtgggctgct
     5461 tgccgcgcgt cgtggcgtcg c
//
LOCUS       DAORGN010000270         5472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7447_length_5472_cov_7.314011, whole genome shotgun sequence.
ACCESSION   DAORGN010000270 DAORGN010000000
VERSION     DAORGN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7447_length_5472_cov_7.314011"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKL17_19885"
     CDS             <1..395
                     /locus_tag="PKL17_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47054.1"
                     /translation="VPRIFVSQKRVQQWTEEGRVHIEGNQMTLPEMGRMFRLTEAFYI
                     EKAVSDGGDSLNLRGRVKTRNQIAQLGGEVFLNSLVIGDAAYEGDPGFVGEPLPKSAK
                     LSASKPDAAPVRNLSSAPTGKVMPKPSV"
     gene            complement(506..919)
                     /locus_tag="PKL17_19890"
     CDS             complement(506..919)
                     /locus_tag="PKL17_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47055.1"
                     /translation="MAEKVAKLNIKRENGYLYFLRGTEVWKTPMKRAGQASPAGKAEK
                     VANGSFNREEGYLYFLDSHGDVSRAKRAVGGQKRKKTKADKKPATKKAPAKKAAPAAK
                     KAAPAAKKPAPAAKKPAPAAKKPAPAAKKPAPKKK"
     gene            complement(1144..2058)
                     /locus_tag="PKL17_19895"
     CDS             complement(1144..2058)
                     /locus_tag="PKL17_19895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNK47056.1"
                     /translation="MTRLFRHVKFPLPPDKRRQYKCRMSQNATALPVEPGARDLYVDA
                     ELYDHEYRRRKADVAWYRQLAKDVAAVQTKKRPILELGSGTGRLLVPLARDGHQVIGV
                     DRSPQMVLRCQQRLSRQSQRVAQNVQLIEADFRALPLDCSPSERFRLIVCPFNGLLHL
                     YERQDLERFLAEVRRLLHPDGLFAFDAANPDPQWLARDPNRRWSRTRFRHPRTGEPLV
                     YSTNHDYDPASQVMTMRLFYEPDAKANWPTPSPPRTVILTQRMYFPAELAALLHYNGF
                     SLWYRAGGFDGQPLSPVSAEQVICARVR"
     gene            2068..2502
                     /gene="ruvX"
                     /locus_tag="PKL17_19900"
     CDS             2068..2502
                     /gene="ruvX"
                     /locus_tag="PKL17_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNK47057.1"
                     /translation="MRILGLDVGSKTIGVALSDELLLCAHPQKTLSRQGTKKDVETLL
                     LLCKQHQVTQVVVGLPYDCEGNEGHRAKRVRVLGDALSAAGLQIEYQDESFSTVEAED
                     ILLAADLSRARRKEVVDRLAAAVILQAWLDSHRPKQSQEESW"
     gene            2502..3605
                     /gene="mltG"
                     /locus_tag="PKL17_19905"
     CDS             2502..3605
                     /gene="mltG"
                     /locus_tag="PKL17_19905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNK47058.1"
                     /translation="MRVSLAMRQRLAALVVFLTVGAAVGVAGHFVWQAFRYPDRPGPA
                     SGPADARIVIVKGMSLSEISRLLADKDLIVHPEWFRFYASERGVASKIRAGAYFLSAR
                     MSPRQILDTLLSGAREPDVPVTIPEGKHMLEVAHLLEEAGICKAAEAERLMRDPSYAL
                     SLGIGGGSLEGYLYPDTYRFPPGVPCTKVLPALVKRSQKVLGELKSQYFEGLRILNRQ
                     YKFGDREIMIMASLVEKETARPEERPRIAGVFLNRLRLPTFKPHRLETDPTIIYGCTV
                     PTEKSEACQQFQGRIRRIHLQDPDNPYNTYTHEGLPPAPICNPGRAALAAVLAPDTTP
                     YLYFVSRNDGTHQFSVTLEEHQAAVNKYQRSGH"
     gene            3692..4798
                     /locus_tag="PKL17_19910"
     CDS             3692..4798
                     /locus_tag="PKL17_19910"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNK47059.1"
                     /translation="MSSSRKPEIAGEVFGRRQGTLFSALSQRVSSGSDEHTALRDYRI
                     LASLVGLAGVVALAAVPALPVWAAPLGLAFGVLYYVIFARRERRRAAVLAQPFPEPWQ
                     KTLSERVPLYLQLTDEEKRRFEGEVAVFLAEQRIYSLGGLEGEAESQVQLTDEHRVLI
                     AASAAILLLGRPDWRLPTERDIVVYPSSFAEETYSMEGGAHTIGMVHAQGPILFAIDA
                     IERSFPRRHSWPSSSQWLQPPLAQSHVGLHEFAHVLDFLGQQGRSTGVPVLLPPSVQA
                     QWHDQLAVERDRLMMGDSVLNPYGLKNEAELFAVSVESFFQDAQRLRLHHPALYKLLA
                     DFFNQDPAERLVPLTPVASFRPWFALGGTTRYIA"
BASE COUNT         1015 a         1582 c         1709 g         1166 t
ORIGIN      
        1 acgtgcctcg gatctttgtt tctcagaagc gtgtgcagca gtggactgaa gaaggccggg
       61 tccacatcga gggcaaccag atgacgctgc ccgaaatggg tcggatgttt cgtctcaccg
      121 aggcgtttta catcgagaag gcggtttctg acggtggcga ttcactgaat ctgcgcggtc
      181 gggtcaagac ccgcaaccag attgcccagc tcggcggcga ggtgttctta aactcgctcg
      241 tcattggcga cgctgcctac gagggcgatc ccggtttcgt cggtgagccg ctgcccaaga
      301 gcgccaaact gtcggcctca aagcccgacg ctgcgccggt acgcaacctc tcgtcggctc
      361 caaccggcaa agtaatgccc aaaccttcgg tctaacgccc cgcctgctac ctgggcaaaa
      421 acagacgcga ctgtaacccc gaaaaaacaa aagggccgac tgcgcatggc agccgaccct
      481 ttaggtacta cttattttcg acgagttact tcttcttggg cgccggcttc ttggcggcgg
      541 gcgccggctt cttggcggcg ggtgccggct tcttggcggc gggcgccggc ttcttggcag
      601 ccggagcagc cttcttggca gccggagcag ccttcttggc cggggccttc ttggtggccg
      661 gcttcttgtc ggccttggtc ttcttgcgct tctgaccgcc gaccgcacgc ttggcgcgcg
      721 aaacgtcgcc gtgcgaatcg aggaagtaca ggtagccttc ctcacggttg aagctgccgt
      781 tggcaacctt ctcagccttg ccagccggcg aggcctggcc agcccgcttc atcggggtct
      841 tccacacttc ggtgccccgc aggaagtaca gataaccgtt ctcacgcttg atgttcagct
      901 tcgcaacttt ctcggccatt actttcctcc atctctcaga ggggttggtt tgaattgaca
      961 ctatttgagg ctcgacgagg ctgtcaattc gttttgtgcg gaaaggttca ctttttttcg
     1021 tcgggcgctt ttttgcaggt gcttgatcct cttcgggaaa gtgccaatta tcgtcggaga
     1081 tagccgtttt ctcgtctgaa tcgccaaatt gggcgatttt cggccattca gacggggttt
     1141 tcttcagcgc acgcgagcgc agatcacctg ttctgcgctg acggggctaa gtggctgtcc
     1201 gtcgaacccc ccggcgcgat accacaaaga aaagccgttg tagtgcaaaa gggcggctag
     1261 ctcggctggg aagtacatcc gttgggtcaa aatcacggtg cgcggtgggc ttggcgtggg
     1321 ccagttcgcc tttgcatcgg gttcatagaa gagccgcatc gtcatcacct ggctcgccgg
     1381 gtcgtagtcg tggttggtgc tatagacgag cggctcgccg gtgcgcgggt gacggaagcg
     1441 cgtcctcgac cagcgacggt tgggatcgcg ggccagccac tgcggatcgg ggtttgcggc
     1501 gtcaaacgcg aacagtccgt cggggtgcag cagccgtcgc acttctgcca aaaaccgctc
     1561 gaggtcctgc ctctcataca gatgaagcag tccattaaag ggacagacga tgagtcgaaa
     1621 gcgctcactt ggcgaacagt cgagcggtag ggcgcgaaag tccgcctcga taagctgcac
     1681 attttgcgcg acgcgctggc tctggcggga cagccgctgc tggcagcgca aaaccatctg
     1741 cggcgagcga tcgacgccaa tcacctgatg tccatcgcgg gccagcggca caagcagtcg
     1801 tcccgttccc gatcccagct cgaggatcgg acgctttttg gtttggaccg cggcgacgtc
     1861 tttggcgagc tggcgatacc atgcgacatc cgcttttcgt cggcggtatt cgtggtcata
     1921 aagctcggca tcgacataca gatcgcgggc gcccggttcg acaggcagcg cggttgcgtt
     1981 ttgtgacatg cggcacttgt actgccttcg cttgtcgggc ggcagcggaa acttgacgtg
     2041 ccgaaaaagc ctggtcatgg tcgctcggtg cggatactcg ggctggacgt ggggagtaag
     2101 acgataggag tcgcgctcag cgacgagctg cttctctgtg cgcaccccca aaaaacgttg
     2161 tcgcggcagg gcacgaaaaa agacgtcgag accttgcttt tgctgtgcaa gcagcatcaa
     2221 gtgacccaag tcgtcgtcgg acttccgtat gactgcgagg gaaacgaagg tcatcgcgcc
     2281 aagcgggttc gggtgctggg cgacgccctt tcagcggcgg gtttgcagat cgaatatcaa
     2341 gatgagagct tttctacagt cgaggcagag gacatcctgc tggccgccga tctgtcgaga
     2401 gcgcgacgca aggaagtggt ggatcgtttg gcggcagcgg tgatcttgca ggcttggctc
     2461 gacagccatc gtccaaagca gagccaagag gagtcttggt gatgcgggtt tcgctggcaa
     2521 tgcgacagcg tctggcggcg ctggtggtct tcttaaccgt cggggcagct gtcggtgtgg
     2581 ctgggcactt cgtctggcag gcgttccgtt atcccgatcg gcccggtccg gcgtcggggc
     2641 ccgcggacgc gcgaatcgtg attgtcaaag gcatgagcct gtcggaaatc tcgcgactgc
     2701 tcgccgacaa agacctgatc gtgcaccccg agtggttccg tttttacgcc agcgaacgcg
     2761 gtgtcgcgtc gaagattcga gccggggcct attttctgtc ggcgcggatg tcgccgcggc
     2821 agatcctcga tacgctgctg tcgggggcgc gcgaaccgga tgtgccggtg acgattcccg
     2881 agggcaagca catgctggag gtggcccatc tcctcgaaga agcgggaatc tgcaaagctg
     2941 ccgaggcgga gcgactgatg cgcgatccaa gctatgcgct gtcgctgggg atcggcggcg
     3001 gctctctcga aggctatctg tatccggata cctatcgttt tccgccgggg gtgccgtgta
     3061 ccaaagtgct gccggcgctg gtcaagcgat cgcagaaagt gctcggtgag ctcaaatcgc
     3121 agtatttcga ggggctgcgc atcctcaata ggcagtacaa gtttggcgat cgcgaaatca
     3181 tgatcatggc gagcctggtc gaaaaagaaa ccgcgcgtcc cgaggagcgg cctcggattg
     3241 ccggcgtgtt tctaaaccga ctgcgcctgc cgacgttcaa gccgcatcgg ctcgagaccg
     3301 atccgacgat catctatggc tgtacggttc cgacggaaaa aagcgaagcg tgccagcagt
     3361 tccagggccg catccgtcgc attcacctgc aagaccccga caacccatac aacacctata
     3421 cccacgaggg gctgccgccg gcaccgatct gcaatccggg tcgcgcggcg ctggcggcgg
     3481 tgctggcacc agacacgacg ccctatctgt attttgtttc gcgcaacgac gggacgcacc
     3541 agttttccgt gacgctggaa gaacaccagg ccgcggtgaa caagtaccag cgcagcggac
     3601 actagcccgg ctggggcgag cgaaaatcga cgattgcaaa gaagtgcaaa gctgccgttg
     3661 cggaaggccg gtgggcgctt agatttgcac tatgtcgagt tcgcgaaagc cggagatcgc
     3721 tggggaggtc ttcgggcgcc gccaaggaac cctgttttcg gccttgtcgc agcgggtgtc
     3781 gtcgggttcc gatgaacata ccgcgctgcg agactaccgc atcctggcga gcctggtcgg
     3841 tctggctggg gtcgtcgcgc tggcagccgt tccggctttg cctgtgtggg ccgcgccgct
     3901 cgggcttgca tttggcgtgc tctactatgt gatttttgcg cgacgggaac gtcgtcgggc
     3961 cgccgtgctt gcgcagccgt ttcccgagcc gtggcaaaag acactgtccg agcgcgtgcc
     4021 gctttacctg cagctgaccg acgaagaaaa gcggcgcttc gagggggaag tcgcggtgtt
     4081 tttggccgag cagcgcatct attctctcgg tgggctggaa ggcgaggcgg agtcgcaggt
     4141 gcagctgacc gacgaacatc gggtgctgat tgcggcgagc gcggcgatcc tgcttctggg
     4201 ccggcccgac tggcggctgc cgacggagcg cgacatcgtc gtctatccgt cgtcctttgc
     4261 cgaggagacc tattcgatgg aaggtggcgc gcacaccatc ggcatggtcc acgcacaggg
     4321 cccgatcctg tttgcgattg atgccatcga gcgcagcttt ccgcgtcggc acagctggcc
     4381 ttcgtcgtct cagtggttgc aaccgccgct tgcccagtcg cacgttgggc tgcatgagtt
     4441 tgcacatgtg ctcgattttc ttggccagca gggacgctcg acgggcgttc cggtgctgtt
     4501 gccgccgagc gtgcaggccc agtggcacga tcagcttgcc gtcgagcgtg accgtctgat
     4561 gatgggtgat tcggtgctaa atccctacgg gctgaaaaac gaagccgagc tgttcgcagt
     4621 ttccgtcgag agcttctttc aggacgcgca gcgactgcgg ctgcaccacc cggcgctgta
     4681 caagcttttg gccgactttt ttaaccaaga tcccgccgag cggctggttc cgctcacgcc
     4741 ggtggcgagc ttccgtccgt ggtttgctct cggtggaacg acgcgctata tcgcatagca
     4801 tatcggttcc attcccatat atgcctacca cccgtttcgt catatctacg taagacaaac
     4861 atccacaccg atccgttcag cgcttgactg ataggccaga cactttcgaa ctgtgtgatt
     4921 tgtgtagaag tgaagttctt atctacacaa ctgcgcagct tggacgtgca agacacggtg
     4981 atccgtacct gcacggagac cctgcgatgc tggacccgtc ctgcgccagc cagcccacag
     5041 gcttccacag cggatctggg aaaacctgtg aataactgtg tgggcgtgtc cgcaaagtcc
     5101 ttggtttaca aggagtttgc tgtgcctgcc taaaaattag gcagccgccc agacgcgctc
     5161 atctctgctt tttggcccgc gatcagcctt acggaaacgg tgcctactta tttctgcgcg
     5221 ccggctccgg ctagattggt cgtaccaatt tgcgaagcaa agagcccagc gctttccatg
     5281 cgtacatctg cgcatttgcc aaaaatcgaa tggggaaaat ttatccctag ggcaaattcc
     5341 tgattggttc cctgtggcta cagacgtcca actcgcttgt gcttttgact tggcaagagg
     5401 tagaatcggg cggtcccaaa agatacctac gcaaaaggcc catacccatg acagatatcg
     5461 tgcaggcact cg
//
LOCUS       DAORGN010000271         5455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7482_length_5455_cov_6.100741, whole genome shotgun sequence.
ACCESSION   DAORGN010000271 DAORGN010000000
VERSION     DAORGN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7482_length_5455_cov_6.100741"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKL17_19915"
     CDS             <1..874
                     /locus_tag="PKL17_19915"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNK47060.1"
                     /translation="VAVDATPQGLVRIRASSTEIGQGKDTVFTQIAQDALGIPSELIE
                     VVRPDTAEVPDSGPTVASRTVMVVGKLVHSACLALRQKLQEAGLVGESYTAAEFVSAC
                     GIFHQLGGVLRVESQYQQPAGIEWDDKKYRGDAYATYAWAVYVAEVTVDLTTYETRVD
                     RFWAVQDAGKIINPVLAAGQIEGGVAQGVGFALYEKVVWQAGRMQNAQMTNYIMPTSL
                     DVPDIFVEFDDRATKMGTATPNGPKGAKGIGELPLDGSAPAVISAVQHATATAVAAVP
                     MTPEDLMQAVETAR"
     gene            871..1344
                     /locus_tag="PKL17_19920"
     CDS             871..1344
                     /locus_tag="PKL17_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNK47061.1"
                     /translation="MKLTMTVNGVERTCDVHPLARLLDVLRRDFQLPGTKEGCGEGEC
                     GACSVFVDGQLVNSCLVPAMQAAGRDVQTIEGVAPSAELIDRVQQAFVQHGGAQCGIC
                     TPGMILAAKALLKEHPQPTVAQIREGLAGNLCRCTGYTKIFESVLAATQQAEEQP"
     gene            1341..2225
                     /locus_tag="PKL17_19925"
     CDS             1341..2225
                     /locus_tag="PKL17_19925"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNK47062.1"
                     /translation="MSHNLSDFVAQPASTLSEALALLGEPSDPQRGPWRPLAGGTDVM
                     VQLGAGQLRPGRYVDIWSIAELRGIRVDEAQIEIGALTTYSEIRKHALLATEFPLLQQ
                     SARETGAIAIQNRGTIGGNIANASPAADTPPSLLVYDAELEIQSVRGVRQVPYSDFHR
                     GYKQMDLAPGELITKVRLKRPSSDEQGRRVHYYRKVGTRAAQAISKICLAGFACVQGD
                     RISQLRIGLGSVAPIPLRCPKTESVLTQGPLTEALIRRAEATLAYEITPIDDVRSTAL
                     YRRKVTLNLLREFASRCL"
     gene            2295..2711
                     /locus_tag="PKL17_19930"
     CDS             2295..2711
                     /locus_tag="PKL17_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47063.1"
                     /translation="MATDHVKLYVDEFAIVSYSPSEGVFRFARTTAPYPSIEAAQASY
                     DALLTVLSAPHAATKSPRLLVDMRAAPARNDPPFEEMQKRYRGQFLKQFVRVATLLTS
                     RAGLLQVQRYGREDGRRDIRGFLNEEEAVQFLRSDD"
     gene            complement(2766..>5455)
                     /locus_tag="PKL17_19935"
     CDS             complement(2766..>5455)
                     /locus_tag="PKL17_19935"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNK47064.1"
                     /translation="EFGVSLVGGCCGTTPAHIAAVAKRVRSEPVKSFYGRPRPEPKVG
                     GQASSLYVPVPLIQEPAPLIVGERSNANGSKKFRELLLREDFEGLVEVGREQVAEGAH
                     LIDVCTAYVGRDEVRDMREVLRRYATQITQPLVIDTTQLDVLEAALQMLGGRAVINSV
                     NLEDGEEKANQVCALARKYGAALVALTIDEDGMAKTAERKLAVASRLYDLAVNRHGLP
                     ATALLFDPLTFTIASGDEDSRNAALETLSGVRAIKENLPGALTLLGVSNVSFGLKPYA
                     RQVLNSVFLSEAILNGLDAAIVSPAKIVPLAKLKPADVELARDLIYDRRSPGYDPLFK
                     FVERFGGESADTSAQKGENGTLEERLSRRIIDGKKVGIDKLLEEALNAGHKPLGIIND
                     ILLSGMKTVGELFASGQMQLPFVLQSAETMKAAVAYLEPFMDKSSGSEKGVMVLGTVK
                     GDVHDIGKNLVDIILSNNGYRVINLGIKQPVDAILEAAEQNKADAIGMSGLLVKSTVV
                     MKENLELMASRGWSIPVLCGGAALNRAYVEGPLSSSYRTGEVYYGVDAFTGLRLMDEL
                     CGHVKPEDRTLTGPGRKRLAGQRISSELHKAEQRAEAWEAAMAYASSDVQSLAQLPLP
                     PFWGAKILEGSAINLRDVFGYINRLVLFRGQWQYRRGRRSEDEYRHFVSETVEPKFYR
                     WCERAIERRYLKPRLIYGYFPCYSDENSLVVLHPPGTPNALQEAERFTFPRQPQGKRL
                     CISDFYRSKARAQKDGFDVLPISLVTMGDTPSQVEADMFAKHRYDDYLHFHGLAVEAT
                     EALAEWLHKRIRHELGIAAADAPLVDQLFQQGYQGSRYSFGYPACPRLEDHQQLFRLL
                     GAERIGVTLSEEFQIVPEQSTAAIISHHPDARYFSITGG"
BASE COUNT         1071 a         1637 c         1675 g         1072 t
ORIGIN      
        1 agtggccgtc gatgccacgc cgcaaggact ggtccgcatc cgtgcatcgt cgacggaaat
       61 tggccagggc aaagacactg tttttaccca aattgcccaa gatgcgctgg ggattccgag
      121 cgagcttatc gaggtggtgc gccccgatac cgcggaagta ccggacagcg gcccgacggt
      181 ggcgtcacgg accgtgatgg tggtgggcaa gctggtccac agcgcctgcc tggcactgcg
      241 acagaagttg caggaagcgg gacttgtcgg cgagtcgtac accgcggcag agtttgtgtc
      301 tgcatgcggg attttccatc agcttggtgg cgtgctgcgc gtcgaaagtc agtatcagca
      361 gccggcgggc attgagtggg acgacaaaaa gtaccgcggc gatgcctatg cgacgtatgc
      421 gtgggcggtc tatgtcgcgg aagtgacggt cgatttgacc acgtatgaga cgcgggttga
      481 ccgtttttgg gcggtgcagg acgccggcaa aatcatcaat cctgtgctgg cggcggggca
      541 gattgaaggc ggcgtcgcgc aaggcgtggg ttttgcgctg tacgaaaaag tggtgtggca
      601 ggcgggccgg atgcaaaacg cccaaatgac caactacatc atgccaactt ctctcgatgt
      661 gccggatatc ttcgtcgagt ttgacgatcg ggcgaccaaa atgggcaccg cgacgcccaa
      721 cgggcccaaa ggcgccaagg gcatcggcga gctcccgctc gatggatcag ccccggcagt
      781 gatttcggcg gtgcagcacg cgaccgcgac ggctgtggcc gcggtgccga tgaccccaga
      841 agacctgatg caagcggtgg agactgcgcg atgaagctga ccatgactgt caacggcgtc
      901 gagagaacct gcgacgttca cccgcttgcg cggctgctcg atgtgctgcg cagggatttt
      961 caactgccgg ggaccaaaga aggctgcggc gaaggcgagt gcggcgcgtg cagcgtgttt
     1021 gtcgacggcc agcttgtcaa cagttgcctg gtgccggcga tgcaagcggc ggggcgcgac
     1081 gtgcagacga ttgaaggcgt agcgccgtcg gcggagctga tcgaccgggt gcagcaggcg
     1141 tttgtccagc atggcggagc gcagtgcggc atttgcacgc ccggaatgat cttggcggcc
     1201 aaggcactgc tcaaagaaca tccgcagccg acggtggccc agatccgcga agggctggcc
     1261 ggcaatctgt gccgctgcac cggttacacc aagattttcg aatcggtgct ggcagcgacc
     1321 cagcaggcag aggagcagcc atgagccaca acctgtcgga ttttgttgcg cagccagcgt
     1381 cgacgctgtc cgaagcactg gcgctgcttg gcgagccgag tgatccccag cgcgggccgt
     1441 ggcgaccact tgcagggggc accgatgtga tggtgcagct tggggccgga cagcttcggc
     1501 ctgggcgtta tgtcgatatc tggtcgattg ccgagctgcg tggcatccgc gtcgatgaag
     1561 cccagatcga aatcggcgcg ctgaccacgt acagcgaaat ccgcaagcac gcgctgttgg
     1621 ccacggagtt tccgctgctt cagcaatcgg cacgcgagac gggtgcgatt gcgattcaaa
     1681 accgcggcac catcggtggc aacatcgcca atgcttcacc ggctgccgat acgcccccgt
     1741 cgctgctcgt ctatgatgcc gaactagaga tccagtctgt gcgcggcgtg cgccaggttc
     1801 cctattcgga ctttcaccgc ggctacaagc agatggacct tgcgcccggt gagcttatca
     1861 ccaaggtgcg gcttaagcgg cccagcagcg acgaacaggg gcgacgggtt cattactatc
     1921 gcaaagtcgg aacacgcgcc gcgcaggcga tatcgaaaat ctgtctggcg gggtttgcgt
     1981 gcgtacaagg cgaccgcatt tcccagcttc gcatcggtct tgggagtgtg gcgccgattc
     2041 ctctgcgctg tccgaagacc gagtcggtgc taacccaagg accactgaca gaagcgctca
     2101 tccgtcgtgc cgaagcgacg ctggcgtatg agatcacgcc gattgatgat gtgcgttcga
     2161 cagcgctgta tcgccgaaaa gtcacgctca atttgctgcg tgaattcgct tcccgctgcc
     2221 tgtaacccgc gattccgaaa gcgaaaacag gccaagtgcc cagcgccgta acgcttggcg
     2281 tatgatgggg cggcatggcg actgatcacg tcaagctgta tgtagatgag tttgcgatag
     2341 tgagctactc accaagcgaa ggggtcttcc ggtttgcgcg gacgactgct ccctatccca
     2401 gcatcgaggc agcgcaggca agctacgatg cgctcttgac ggtgctgtcg gcgccgcacg
     2461 ccgcgaccaa gtcgccacgg ctgctggtcg acatgcgcgc agcaccggcc cgcaatgatc
     2521 caccgtttga ggagatgcag aaacgctacc ggggccagtt tctgaaacag tttgtgcgcg
     2581 tcgcaacgct gctcacgtcc cgtgccggcc tgctccaggt gcagcgctat ggccgggaag
     2641 atggacgtag agacattcgc ggctttttga acgaagaaga agcggtgcag tttctgcgca
     2701 gcgacgatta gtccccgctg gtgacacaga gcggggaagc acggtccaga ctccgcagcg
     2761 caggctcagc caccggtgat gctgaagtag cgcgcgtcgg ggtggtggct gatgatcgcg
     2821 gcggtcgact gctcggggac aatctggaac tcttcagaca gcgtgacgcc gattcgttcc
     2881 gccccaagca aacggaaaag ctgctgatga tcttcgagcc gcggacaagc cggatagcca
     2941 aaggaatagc ggctcccttg atagccttgc tggaagagct gatcgacgag cggcgcatcc
     3001 gcagcggcaa ttcctagttc gtgccgaatc cgcttgtgga gccactcagc cagcgcttcg
     3061 gtcgcttcca ccgccagacc atggaagtgc agatagtcgt cgtaacggtg cttggcgaac
     3121 atgtctgctt ccacttggga cggcgtatcc cccatcgtca ccagcgaaat cggcagcacg
     3181 tcaaagccgt ccttctgcgc gcgcgctttg ctgcgataga agtcggaaat gcacagacgc
     3241 ttgccctgcg gctgacgtgg gaatgtgaat cgctcggctt cctgtagcgc attgggagtc
     3301 cccggaggat gcagcacaac caagctgttt tcgtcggaat agcacgggaa gtagccgtag
     3361 ataagccgcg gttttaggta gcgccgttca atcgctcgct cgcaccacct gtaaaacttc
     3421 ggctcgaccg tttcgctgac aaagtgacgg tattcatcct cggaccgacg cccgcgacgg
     3481 tactgccact gaccgcggaa cagcaccaag cgattgatgt aaccgaatac atcgcgcagg
     3541 ttgattgcgc tgccttccag aatcttggca ccccaaaacg gcggaagagg aagctgcgca
     3601 agcgactgaa cgtccgacga tgcatatgcc atcgcggctt cccaggcttc ggctctctgt
     3661 tcggctttgt gaagctcact ggaaatgcgc tgcccggcga gccttttgcg tcccggtccg
     3721 gtcagcgtgc ggtcttcggg tttgacgtgt ccgcacagct catccatcag ccgcagaccc
     3781 gtgaaagcat cgacgccgta atagacttcg ccggtgcgat aggaagacga cagcggtcct
     3841 tcgacataag cgcggttgag cgcagccccg ccacagagca cgggaatcga ccagccacgg
     3901 ctcgccatca gttccaggtt ttccttcatc accaccgtcg atttgacgag cagtccagac
     3961 attccgatcg catcggcttt gttctgctct gccgcttcca aaatggcatc gacaggctgc
     4021 ttgattccca ggtttatcac ccgatagccg ttgttcgaca ggatgatgtc gacgaggttt
     4081 ttgccgatgt cgtggacgtc tcccttcacc gtgccgagca ccatcacgcc cttttccgaa
     4141 ccgctcgact tgtccataaa cggttccagg tacgcgaccg ccgctttcat cgtttcggcg
     4201 gattgcagaa caaacggaag ctgcatttgt cccgaagcga acagctcacc gacggttttc
     4261 atccccgaaa ggaggatgtc attgatgatc ccaagcggct tgtgtccggc gtttagcgct
     4321 tcttccagca gcttgtcgat gccgactttt ttgccatcaa tgatgcgccg cgacagtcgc
     4381 tcttccagcg tgccgttttc ccccttctgc gccgaggtgt ctgcactttc accgccgaat
     4441 cgttcgacaa acttgaacag cggatcgtat cccggtgaac gcctgtcata gatcagatcg
     4501 cgcgccagct cgacgtcggc gggcttgagc ttggccagcg gcactatctt ggccggactg
     4561 acaatcgccg cgtcgagtcc gttcaggatc gcttccgaaa ggaagacgga gtttaagacc
     4621 tgccgcgcat acggtttcag tccaaagctc acgttcgaca ccccgagcag cgtcagcgcg
     4681 cccggtaggt tttccttgat ggcgcgaaca ccggacagcg tttccagcgc ggcgttgcgg
     4741 ctgtcttcat cccccgaagc gatcgtaaac gtcagcggat cgaacagaag cgccgtcgct
     4801 ggcaagccat gtcggttgac cgccaagtca tacaaacgac tggcaaccgc cagcttgcgc
     4861 tctgcggtct tggccattcc gtcttcatca atcgtcagcg caaccagcgc ggcaccatac
     4921 tttcgtgcca gcgcgcagac ttggttggct ttttcttcgc cgtcctcgag gttcacggag
     4981 tttatgaccg cgcgaccacc gagcatttgc agcgccgctt ccagtacatc aagctgcgtt
     5041 gtatcgatca ccaaaggctg ggtaatttgg gtggcgtagc gacggagcac ttcgcgcata
     5101 tcccgcactt catcgcgccc gacatacgcg gtgcagacat caatcaggtg cgcgccttcc
     5161 gccacttgtt cgcgccccac ttcgacgagc ccttcgaagt cttcgcgcag gagcagctcg
     5221 cggaatttct tgctgccgtt tgcgttgctg cgttcgccca cgatgagcgg cgcgggttcc
     5281 tgaatcagcg gaaccgggac atacagcgac gacgcttggc ctccgacttt cggttccgga
     5341 cgcggcctgc cgtaaaacga cttcacgggc tcgctgcgca cgcgctttgc caccgcggca
     5401 atgtgcgcgg gcgtggtgcc gcagcagccg ccgaccaaag acacgccaaa ctctc
//
LOCUS       DAORGN010000272         5392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7615_length_5392_cov_6.080195, whole genome shotgun sequence.
ACCESSION   DAORGN010000272 DAORGN010000000
VERSION     DAORGN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7615_length_5392_cov_6.080195"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1318)
                     /gene="odhB"
                     /locus_tag="PKL17_19940"
     CDS             complement(98..1318)
                     /gene="odhB"
                     /locus_tag="PKL17_19940"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNK47065.1"
                     /translation="MSIELIIPPLGESISEAVVAKWLKPVGALVKVDDPLAELETDKV
                     TVTLPAPSAGVLVEQLVPVGTSVRVGSSIGRIDPTAQPSQVQPSTTEPAKKPTPVEPA
                     KAAVPSPAPAPVAAPLPAAAPAPSPPARPQLTPNTRKLLREQGVDPSTAAASVQVAPP
                     RAKAAPAPAVPSSAGPEEVVTLSSLRKRIAERLVEAQHTAAILTTFNEVDMSYIMALR
                     SKFQDEFVKKHGIKLGLMSFFVKACVEALREFPGLNAEMRGDAIAYKKFYDFGIAVGS
                     GKGLVVPVLRGVDKLGLADIEKGIAELAQKAKENKLQLSDLAGGTFSISNGGVYGSMM
                     STPLLNMPQTGILGMHNIIRRPIAVGDNIEIRPMMYVALSYDHRVVDGREAVSFLVRV
                     KERLESPEKFLLGI"
     gene            complement(1360..4131)
                     /locus_tag="PKL17_19945"
     CDS             complement(1360..4131)
                     /locus_tag="PKL17_19945"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNK47066.1"
                     /translation="MNASDLKLVGLLGANLRYLDEQYELSQTSPSEVEPSFAALLDSG
                     DASFFASQTGATAPKQTSGHADTLPYRVQSLVAAFRRYGHLAAQLDPLGLITPVGNPE
                     LDPSAHGLGPDDLQRPVHGLTLAGIPDGSTVSAIVDHLKTVYSSSIGAEVMHIDDPRR
                     RQFVLDRLEGQTNRFSHSRDEQIHILRRLADAEILEQFLHTKYVGAKRFSLEGGQTLI
                     PLLDYLFQLAGERGLSQAVIGMAHRGRLNVLVNLLGKQPRLLFAEFEDIQAETVMGSG
                     DVKYHMGYSTEFVTRSGQNIHLSLAFNPSHLEAVNPVVVGRVRAKQQRLHDSERSKVL
                     GVLVHGDAAFAGQGLVTETLSLSGLPAYQTGGTFHVIVNNQIGFTTDPSDSRSTRYCT
                     DVAKLLQAPILHVNGHDPEAVIHAVEILLDYQRQFGCDVVLDMVCYRRYGHNESDEPG
                     FTQPLMYQRIDQLSSVRSLYAQSLAKRGVLPQSEADAMVQRKQAELELELVAAKSASE
                     RPVVNAGDGVWRGYRGGPDASVPDADTSVSQADLADLAQRLSTVPAGFSAHPKIERLL
                     QLRAAMGRGEQGLDWGMAELLAFGSLLREGTLVRLTGQDSRRGTFSQRHAVLTSYKTG
                     EKFTPLCHLSSKLGPVGEFQVHDSLLSEAAVLGFEYGFSLDYPDALVLWEAQFGDFVN
                     GAQVILDQFISSAEDKWRRLSGLTMLLPHGYEGQGPEHSSARFERFLQLCAEDNWQVT
                     YPTTPAQYFHLLRRQVKRPWRKPLVVMSPKSLLRHPAATSPISELSQGGFQCVLQDTK
                     VSADKVQRIMLCTGKIYYDLVDERKRRSDGQTAICRIEQLYPFRVPDISALLVQYPSA
                     REMIWVQEEPANMGAQSFVAPRLLALLPANVQLRFASRVESASPATGSYKAHVLEHKK
                     LMDEAFATP"
     gene            4399..4833
                     /locus_tag="PKL17_19950"
     CDS             4399..4833
                     /locus_tag="PKL17_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNK47067.1"
                     /translation="MPANDPQTFGDTLAAVNAGLNSLSAVFLLAGYGFAKAHKPAAHK
                     KCMFAALVVSAVFLACYLTRFYLTGAHRYSGDGLMRIVYFGLLFSHMTLAAAVPPLAL
                     RAVWLAWKGRIDDHRKIVRYAWPVWLYVSVTGVLVYLLLYKL"
     gene            4980..>5392
                     /locus_tag="PKL17_19955"
     CDS             4980..>5392
                     /locus_tag="PKL17_19955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47068.1"
                     /translation="MATLEENSVVGPYRIIRKLGQGGMGSVYEALHQTLGRHVAIKVL
                     HPEVARNEEFALRFANEARASNIVDHPDVVQITDHGSLPDGIPYIVMEYLKGESLGHR
                     IEHLGGPMNPSEVAYIALQVADVLAAAHIKGIIHR"
BASE COUNT         1028 a         1605 c         1664 g         1095 t
ORIGIN      
        1 tgcatcgtcg ccaaaccaaa aagtggccca gcgtccgtta tgtgaacggt gactgttgcg
       61 aggctggaga gggaattgcg gggggcgagg cggggcgcta gatgccgagc aggaactttt
      121 ccggcgattc caggcgttct ttgacccgga ccaggaacga aacggcttct cgaccatcga
      181 cgacgcggtg atcgtaagac agcgcgacgt acatcatcgg acggatttcg atgttgtcgc
      241 cgacggcaat cgggcggcgg atgatgttgt gcatcccgag aattccggtc tgcggcatgt
      301 tgagcagcgg cgtgctcatc atcgatccgt agacaccacc gttcgagatg ctgaacgttc
      361 cccccgcgag atcagagagc tggagcttgt tttctttcgc tttttgcgcc agctcggcga
      421 tgcctttttc gatgtctgcg aggccgagct tgtcgacgcc acgcagcacc ggcaccacga
      481 gtcctttgcc actgccgacg gcgatgccaa agtcatagaa cttcttgtat gcaatcgcgt
      541 cgccgcgcat ttcggcgttc agacctggga attcgcgcag cgcttcgacg caggctttga
      601 cgaagaacga catcagaccg agcttgatgc cgtgcttttt gacaaactcg tcttggaact
      661 tgctccgcag cgccatgatg tagctcatgt cgacctcgtt aaaggtcgtc aaaatggcgg
      721 cggtgtgctg ggcttcgacg agccgttcgg caatccgctt gcgcagagaa gacagcgtca
      781 caacttcttc tgggcccgcc gacgaaggaa cagcgggggc cggggccgct ttggcgcgcg
      841 gtggggcgac ctgaacgctg gcagcggcgg tgctggggtc aacgccttgc tcgcgcagca
      901 gcttgcgggt atttggcgtc agctgtggac gtgcgggcgg actgggggca ggcgcagcgg
      961 ctggcaaagg cgcagcgacc ggagccgggg caggggacgg gaccgctgct ttggctggct
     1021 caacaggggt cggttttttt gctggctcgg tggtgcttgg ctgaacctgg ctcggctgcg
     1081 cagtcggatc aatccgtcca atcgacgatc ccacccgtac cgatgtgccg actggtacga
     1141 gctgttcgac gagaacaccc gcacttgggg ccggcagcgt caccgtcact ttgtcggttt
     1201 ctagctcggc gagcggatca tcgactttga ccagcgcacc gacgggcttt agccacttgg
     1261 caaccaccgc ttccgagatc gattctccga ggggcgggat gatgagttca atcgacatgt
     1321 cgtttcttcc tctatcggca cgaccgcttc gtcgtcacat tacggggtgg caaaggcctc
     1381 atccatcagc tttttgtgct cgagcacatg ggctttgtac gaacccgtgg ccgggctggc
     1441 gctctcgacg cgactggcaa accggagctg cacgttcgcc ggcagaagcg ccaagagccg
     1501 tggcgcaaca aacgactgcg cacccatgtt ggccggctct tcttgcaccc aaatcatctc
     1561 gcgtgcggag ggatactgca ccagcagcgc gctgatgtcc ggcacccgga acggatagag
     1621 ctgctcgatg cggcagatgg cagtttgccc gtcgctgcgc cgctttcgtt catcgaccag
     1681 atcgtaataa atcttgcccg tgcagagcat gatgcgctgg accttatcgg cgctgacctt
     1741 ggtgtcttgg agcacgcact gaaaaccgcc ctggctcagc tcggagatcg gcgacgtcgc
     1801 tgccggatgg cgcagcaggc tcttgggcga catcacgacc agcggcttgc gccatggacg
     1861 cttgacctgc cgccgcagca gatggaaata ctgcgcgggt gtcgtcggat aggtgacttg
     1921 ccagttgtcc tcagcgcaga gctgcaaaaa gcgctcaaag cgggcgctcg agtgttcggg
     1981 cccctggcct tcgtacccgt gcggcagaag catcgtcagg cccgacagcc gtcgccactt
     2041 gtcttccgcc gagctgatga actgatcgag aatcacctgc gcgccattga caaaatcgcc
     2101 aaactgtgct tcccacagca ccagcgcgtc aggataatcg aggctgaagc catattcaaa
     2161 accgagcacc gccgcttccg acagcaagct atcgtggacc tgaaattctc cgacgggacc
     2221 gagcttgctg ctcagatggc acagcggcgt aaacttttcg cccgttttgt agctcgtcag
     2281 caccgcatgg cgttggctga acgtgccgcg acgggaatct tgcccggtga ggcgaaccag
     2341 tgtgccctcg cgcagcaggc tgccaaacgc gagcagctcg gccatgcccc agtcgagccc
     2401 ttgctcgccg cggcccatgg cggcgcgaag ctgaagcagc cgttcgattt tcgggtgggc
     2461 cgaaaagcca gcggggaccg tcgacagacg ctgcgccaga tcggccaaat cggcctgcga
     2521 tacgctggta tcggcatcgg gcacgctggc gtcaggaccc ccgcgatagc cacgccacac
     2581 cccgtcgcca gcattgacca ccggacgttc ggacgccgac ttggccgcga cgagttccag
     2641 ctccagctcg gcctgcttgc gctgcaccat cgcatcggct tcgctttgtg gcagcacgcc
     2701 gcgcttggcc agcgactgcg cataaagcga tcgcaccgac gacagctgat cgatgcgctg
     2761 gtacataagt ggctgggtaa agccgggttc gtcggattcg ttgtgaccgt agcgacggta
     2821 acaaaccatg tcgagcacga catcgcagcc aaactgccgc tgatagtcga gcaggatctc
     2881 gacggcgtgg atgaccgctt cgggatcgtg tccgttgacg tgcagaatcg gcgcttgcaa
     2941 cagcttcgcg acgtcggtgc agtagcgggt cgaacgcgag tcgctaggat cggtggtaaa
     3001 gccgatctgg ttgtttacga tgacgtggaa cgtgccaccc gtctgatacg ccggcagacc
     3061 cgacagactg agcgtttccg tgaccagtcc ttgacccgcg aacgccgcgt cgccgtggac
     3121 cagcacgccc aagactttgc ttcgttcgct gtcgtgtagc cgctgctgct tggcgcggac
     3181 gcgaccgacg acgacgggat tgactgcttc caggtggctt gggttaaacg ccagcgacaa
     3241 gtgaatgttc tgcccgctgc gcgtcacaaa ctccgtcgag taccccatgt ggtacttgac
     3301 gtcgccggaa cccatcactg tttcggcctg aatgtcctcg aactcggcga acaagagccg
     3361 tggctgcttg cccagcaagt tcacaagaac gtttagtcgt cctcggtgcg ccatgccgat
     3421 gacggcttgc gaaagcccgc gctcgccggc cagctggaac aagtagtcga gcagcggaat
     3481 cagcgtctgc ccaccttcga gagaaaagcg cttggccccg acgtacttgg tgtgcagaaa
     3541 ctgctctaga atttcggcat cggccagtcg acgcaggatg tgaatctgtt cgtcgcggct
     3601 gtgtgaaaaa cggttggtct ggccttcgag tcgatccagg acgaactgtc gccgtcgcgg
     3661 atcgtcgatg tgcatgacct cagcgccgat gctgctgctg tagacggttt ttaggtgatc
     3721 gacaattgcc gataccgtcg atccgtcggg gattccggcg agcgtgagac cgtgcaccgg
     3781 acgctgtaga tcgtcggggc caagaccatg cgcgctgggg tctagctcgg gattgcccac
     3841 cggggtgata aggcccagcg gatcgagctg cgcagccaag tggccatagc gacggaacgc
     3901 agcaaccaag ctctggaccc tgtacggcag cgtgtccgca tgtccgctcg tctgcttcgg
     3961 tgctgtcgcg ccggtttgag aagcgaaaaa cgacgcatct ccactgtcga gaagtgccgc
     4021 aaagctgggc tccacttccg acggtgaagt ctgcgaaagt tcatactgtt catcgaggta
     4081 acgaaggttg gctccaagaa gtccaaccag cttgagatcc gacgcattca tcgtgaattt
     4141 gctcgcacgg gacgcccccg cgattctgtc tgaatgttcc gaagaatact cggaaggggg
     4201 tcgcgggtaa acgcctaggc cgttcttctt tgtcagatgc cgacctaggc cgttttgtcg
     4261 ccgcggtttt gaaccggccc atcaccgtga tttttcgacg gagacacttg tttttctccg
     4321 ccgcttcgat ccacactgcg cgcccgcaag gccaaatgcc cgtcgaccat ccaattgtgg
     4381 ccttgcagga ggactgctgt gcccgcaaac gatccgcaaa ctttcggaga caccctggcc
     4441 gcggtaaacg ctggtttgaa cagcctaagc gcggtctttc tgctcgctgg ttatggtttt
     4501 gccaaagcgc acaagccggc tgcacacaaa aagtgcatgt ttgccgcgct cgtcgtttcg
     4561 gcggtgtttt tggcctgcta cctaacacgt ttttatctga ccggggcgca tcgctacagc
     4621 ggcgacggtc tgatgcgcat cgtctatttt gggctgctgt tttcccacat gacgctggcg
     4681 gcagcggttc cgcctctcgc actgcgggcg gtgtggttgg cgtggaaagg ccggatcgat
     4741 gaccaccgca agatcgtccg ctatgcctgg ccggtgtggc tgtatgtctc ggtgacgggc
     4801 gtgcttgtgt accttctact gtataagctg tagtctcgct tgtgcccggc gggccgcgcc
     4861 atgtagtcga cggaacgcgg acaattcgca cattgaccgt ctttcccgag ggtgggaaaa
     4921 tgagtagtaa gcgccacggc attttcgctg gcacttagag actcttttca gagaaaaaca
     4981 tggcgacgct cgaagagaat tcggtagttg gcccctaccg aatcattcgc aagctggggc
     5041 agggcgggat ggggtcggtg tacgaagcgc tgcaccagac gctgggccgt cacgttgcaa
     5101 tcaaagtcct tcaccccgag gtggcccgca acgaagagtt tgcgctgcgt tttgccaacg
     5161 aggcgcgggc gagcaacatc gtcgaccatc ccgacgtggt gcaaatcacc gatcatggct
     5221 cactgcccga cggaattccg tacatcgtga tggaatacct caaaggcgaa tcgctcgggc
     5281 atcgcatcga gcacctcggc ggtccgatga acccgtcgga agtcgcgtat atcgcgctgc
     5341 aagtcgccga cgtcctggcc gccgcccaca tcaaaggaat cattcacaga ga
//
LOCUS       DAORGN010000273         5378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7644_length_5378_cov_6.207026, whole genome shotgun sequence.
ACCESSION   DAORGN010000273 DAORGN010000000
VERSION     DAORGN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7644_length_5378_cov_6.207026"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKL17_19960"
     CDS             <1..703
                     /locus_tag="PKL17_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47069.1"
                     /translation="QSSSPPASSALSPVDDGYGYRPPPPPPPPPPPPKPERETISRSY
                     FQFTMGFLAGGRTYANSSFENTNGVAANLSEPFLKAPFDYETVLGLRYDLRVVYSYIR
                     GTIGVDFPFSTFAARNAMGTYTLDGRTVNVAIQSLRPYELRFGIGGEYPIWVFAPFVD
                     LIGYAYWTNVGAAVNDAKAEFQARGFGFSARAGTRLHVKPWFFVQLAGDVGIYGPTRW
                     NAELSLGFSAPAKRY"
     gene            complement(700..1665)
                     /locus_tag="PKL17_19965"
     CDS             complement(700..1665)
                     /locus_tag="PKL17_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47070.1"
                     /translation="MTTTALSATLETSTELHALLRKIDPASFHESIEADARAQLQRII
                     QRLQWLREASQDDLGKPGHDALPDKLGQLVEALERAQRIETATARAYWAAFQREVHPA
                     YESLAAWLRTAHVAPPSLRPTNYSRNLFHIGSALTALVVILLAPSQGYLIAASAALCL
                     TAWSLEIARRFRPDLNDRLMGFFGKVAHVHERYRVNSSTWYVTALLILAVATPKAVAA
                     LAVVVLGIGDPIAALVGRRFGKTRLRAGRSLEGSLGFVLSASLGSLVVAAMMMPGGIG
                     RWALIAMVAGFAGAGAEVFSTKLDDNLTIPLAVAAAVTALIATGV"
     gene            1755..3026
                     /locus_tag="PKL17_19970"
     CDS             1755..3026
                     /locus_tag="PKL17_19970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK47071.1"
                     /translation="MSSAENPPSAAAIPWTYRWAVLALLSVSLFANYYLFDSLGMVAD
                     VVMREHGLSEVDYGLLSGAYNIAGALALLVGGSLIDRLGTKRSIVLFGALSALAGLLT
                     FAAPHFYLLLLARFILGLGGEPLGVAVSTALARYWKSKDLAFAFGVNLTFARLGTVAV
                     NRSPQWAPSAYAQATSRPALLIAAGIGLISLVAGVLNSRLEARAEQRLSLPPSGRQDR
                     LSFGELRSFDVSYVYLTLVCLGFYCALFPFQSFATKIFIESHRLSRQDAGALLSYLPT
                     TAMFLAPLFGLIVDRSGKRAVLLLCGLLLVAPVFPLLAIPSFPPQLPVLLLCLSFALI
                     PAVLWPSVAMLVEENRLGTAYALMTLLQQLALWVTSALLGLCNERAQASAQHPQGYAP
                     GLWLLGGLCWVALLAAFLLFWRERKRQPRRF"
     gene            complement(3045..4097)
                     /locus_tag="PKL17_19975"
     CDS             complement(3045..4097)
                     /locus_tag="PKL17_19975"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNK47072.1"
                     /translation="MSVRMAQQFSLFAAPDPASQSEPEKASARQKSSHSFDPETLPIP
                     PKLGIPTRSFGTSSWVYPGWDGGVYRSVKAYGASSRFSDLCLAEYARNSLFRSAGADN
                     MYYVRPSQRRKLLQKYASQLRHLPEKVELCPKVFHELSVSRYTPQQQAEWRLLDEINP
                     HFLDPRLFLEEVAQPLCDELGELLGPLILELQENDIHEADFCRLLDHFLQQVRKQFSG
                     PLSVELRTESHFTSRYLSVLATHGVSHVLNSWTKMPSIGQQFALLQQSQAKWPFYLLR
                     ALLPQGVRYAEASKWAPYDRLRVRADSVRKDMLQVLRSLKVEEKAYVLVNNHLEGHAP
                     TTVAEIQHELFSTDSL"
     gene            4163..4810
                     /locus_tag="PKL17_19980"
     CDS             4163..4810
                     /locus_tag="PKL17_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47073.1"
                     /translation="MLRTSRKLVVRFLGFLLSLLLLFRSATAAPPKEADFRRVTDWLW
                     LLAGAATGFVAHEGGHLLLDLAAGTKPEFHKVTLGPFPFFAIQPTVTDLSPRLRYAIP
                     AMGFAAQDLYSELILGIDPRLREHHHPFLKGMLGFHAVLSVGYAITGFAAIGPAQSDV
                     NSMARALQVPPWAIGMMILVPAGCDVYRYFVPDSRWAPWVSLSAKLTTIGAIAAF"
     gene            4904..>5378
                     /locus_tag="PKL17_19985"
     CDS             4904..>5378
                     /locus_tag="PKL17_19985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47074.1"
                     /translation="MSEPRLGPYRLIRKLSQGGMGAVYEGFHEAVERHVAIKVLRPEF
                     MSNSDFVTRFFNEAKAANRIGHPSIVQIFDCGRLTDGTSYIVMELLDGETVGMRIRRS
                     GGRLPLLGALQVVRQVANALAAAHAKNIVHRDLKPDNIMLVRDPAVPGGERVKLLD"
BASE COUNT          971 a         1609 c         1597 g         1201 t
ORIGIN      
        1 tcagagctcg tctccgccgg ccagttcggc gctttcacct gtcgatgacg gctatggcta
       61 tcgtccgccg ccgccacccc cgccgccgcc acccccaccc aaacccgaac gcgagacgat
      121 atctcgcagc tatttccagt tcacgatggg ctttttggct ggtggtcgca cctatgccaa
      181 ctcgtcgttt gaaaacacca acggcgtggc tgcaaacctc agcgagccgt ttctgaaagc
      241 gccgttcgat tacgagacgg tgctgggtct gcgctacgac ctccgcgtcg tctattcgta
      301 cattcgcggc accatcggcg tcgacttccc gttttcgacg tttgctgctc gcaacgcgat
      361 gggaacgtat acgctcgacg gacgcacggt caacgtcgcg atccagtcgc tgcgccctta
      421 cgagctgcgt tttggcatcg gcggtgaata cccgatctgg gtgtttgccc cgtttgtcga
      481 tctgatcggc tacgcctact ggaccaacgt cggcgccgcg gtaaacgatg ccaaagcgga
      541 attccaagcg cgcggctttg gcttttcggc ccgggccggc acgcgacttc acgtcaagcc
      601 gtggtttttc gtccagcttg ccggcgacgt tggcatctac ggtccgactc gctggaacgc
      661 cgagctgtcg ctgggctttt cggcccccgc caagcgttac taaaccccgg tcgcgatgag
      721 cgccgtcacg gcggcggcca cagcgagcgg aatcgtcaag ttgtcatcga gcttggtaga
      781 aaacacttcc gcccccgccc ccgcaaagcc tgccaccatc gcgatgagtg cccatcgtcc
      841 gattcctccc ggcatcatca tcgccgccac cacgagcgat ccaagcgacg ccgacagcac
      901 aaaccccagg ctgccttcca gcgatcgacc tgcgcgcagc cgggttttgc caaaccgtcg
      961 tcccacaagc gccgcgatcg gatcgccgat gcccagcacc accaccgcga gcgccgcaac
     1021 cgctttgggc gtcgccaccg ccaagatcag cagcgccgtc acataccacg tcgaggagtt
     1081 tacgcgatag cgctcgtgga cgtgcgccac cttgccaaaa aagcccatca ggcgatcgtt
     1141 cagatcgggc cggaagcggc gcgcgatttc caagctccat gccgttagac agagtgccgc
     1201 cgacgctgca atcaggtagc cctgcgacgg agcgagcaga atcaccacga gcgccgtcag
     1261 cgccgatccg atatggaaca ggttgcgaga atagttggtc gggcgcaggc tcggcggcgc
     1321 aacgtgcgcg gtacgcagcc aggccgccag cgattcatag gcaggatgta cttcgcgctg
     1381 gaaggccgcc caataggccc gcgctgtcgc cgtttcgatg cgctgggccc gctccagggc
     1441 ctcgacaagc tgcccgagtt tgtccggcag ggcatcgtgg cccggtttgc ccaggtcgtc
     1501 ttgcgaggct tcccgcagcc attgcagccg ctggatgatg cgctgaagct gcgcccgcgc
     1561 gtcggcttcg atcgattcat ggaacgacgc cggatcaatt ttccgcaaaa gtgcatgcag
     1621 ctcggtcgat gtctccagcg tcgcggatag ggcagtggtc gtcaccgtgt tattgaacat
     1681 ttcggcgtgt cgcggcggga acacaaatct gtcaccgttt tgtcacggcc caaactaggc
     1741 tagcttccct ggccgtgtcg tctgccgaaa atcccccaag tgccgctgcg attccgtgga
     1801 catatcgctg ggccgttctt gcactactca gcgtgtcgct gtttgcgaac tactacctgt
     1861 tcgattcgct cgggatggtc gccgacgttg tgatgcgcga gcacgggtta agcgaagtcg
     1921 attacggcct tctgtctggc gcgtacaaca ttgctggcgc gctcgcgctg ctcgtcggag
     1981 gttcgctgat cgatcggctt ggtacgaagc gatcgatcgt gctgtttggg gcgctgtcgg
     2041 cgctggccgg acttctgacc tttgccgcac cgcacttcta tctgctgctg ttggcgcgct
     2101 tcatcttggg tttgggtggt gaaccacttg gtgtcgcggt gagcacggcc cttgcccgct
     2161 attggaaaag caaagacttg gccttcgcat tcggggtcaa cttgacgttt gcacggctcg
     2221 gtacggtcgc tgtcaatcgt tcaccgcagt gggccccttc ggcgtatgca caggcgacct
     2281 cgcggccagc gctgctcatc gcggcgggaa tcggcctgat cagcctggtg gctggcgttc
     2341 tcaattcccg gctcgaagcg cgggccgagc agcggctctc actgcctccg tctggacgcc
     2401 aggaccgact gtcttttggc gagctgcgca gcttcgatgt tagctacgtt tacctcactt
     2461 tggtttgtct gggtttttac tgcgcgctgt ttccgtttca gtcgtttgcg accaagattt
     2521 tcatcgaatc acatcgtctc tcacgccaag atgcgggggc gcttttgagc tacctaccga
     2581 cgacggcaat gtttctggcg ccgctgttcg gactgatcgt ggatcgctcg ggcaagcgcg
     2641 cagtcctgct tttgtgcgga ctccttttgg tggcgccggt ctttcccttg ctggcgattc
     2701 cgtcttttcc gccgcagctt ccggtgttgc tcctgtgtct gtcctttgcg ctgattcctg
     2761 cggtgctgtg gccgtcggtg gcgatgctcg tcgaggaaaa ccgactggga accgcgtatg
     2821 cgctgatgac gttgcttcag cagcttgcgc tgtgggtcac ttcggcgctg ctgggtctgt
     2881 gtaacgaacg agcgcaggcg agcgcgcaac atccacaagg ctatgcaccg ggtctgtggc
     2941 tccttggcgg actgtgctgg gtcgcgcttt tggcggcctt tctgcttttt tggcgcgagc
     3001 gaaaacgtca gccgcggcgc ttctaaatca gattcagtcc aacatcacaa agagtcggtt
     3061 gaaaacagtt cgtgctggat ttctgcgacg gtggttggcg cgtgtccttc cagatgattg
     3121 ttgacaagga cataggcttt ttcttccacc ttcaggctgc gcaaaacctg caacatgtct
     3181 ttccgaacgg agtccgcgcg cacacgaagt cgatcatagg gagcccactt ggacgcctct
     3241 gcataacgga ctccctgcgg caacagagcc cgcagaagat agaacggcca cttggcctga
     3301 ctttgctgca aaagcgcaaa ctgctgaccg atggatggca tcttggtcca ggaatttagg
     3361 acatgcgaaa caccatgggt cgccaagaca gacagatagc gggaagtgaa gtgtgattcg
     3421 gtgcgtagct cgacggaaag tggcccggaa aactgcttgc ggacttgctg taaaaagtga
     3481 tcgagcaagc ggcagaaatc ggcttcgtga atgtcgttct cttgcagctc cagaatcagc
     3541 ggacccagca gctcgcccag ctcatcgcag agcggctgcg ccacttcttc cagaaacagt
     3601 ctgggatcca gaaagtgcgg attgatttcg tctaaaagac gccactccgc ttgctgctgc
     3661 ggggtgtagc ggctgaccga aagttcgtga aacacctttg gacagagttc aactttttcc
     3721 ggcaaatgac gaagctgact ggcgtatttt tgcagcagtt ttcgtcgctg ggaaggccgt
     3781 acatagtaca tgttgtcggc gccggcactg cggaacagcg aattgcgcgc gtattccgcc
     3841 aagcacagat cggaaaaacg actgcttgcg ccgtatgctt tgacgctacg gtagaccccg
     3901 ccatcccagc ccggatacac ccaagagctt gtaccaaagc tgcgggtggg aatgcccagc
     3961 ttgggcggaa tcggcagcgt ctctggatca aaggaatggc tgcttttctg tcgggcagat
     4021 gccttttccg gttcgctttg ggaggctgga tcgggggccg caaaaagtga aaactgctgc
     4081 gccatgcgca cgctcatgcc acagtttcgg tgtggacgac aagcgttgac tcgcagggtg
     4141 cttttgtgcg agcccagaga tcgtgcttcg aacaagccgc aaacttgtgg tccgtttttt
     4201 gggattcctg ctttcgctgc tgctgctttt ccgcagtgcc acggctgccc cacccaaaga
     4261 ggcagacttt cgtcgcgtga ccgattggct gtggcttttg gctggtgcag cgacgggttt
     4321 tgtcgcgcac gaaggtggac acctgctgct ggatttggcg gcaggaacca aaccagagtt
     4381 tcacaaagtg acgctcgggc cgtttccttt ttttgcgatt caaccaaccg tgacagacct
     4441 ttcgccacga ctccgttatg cgattccggc gatgggtttt gccgcgcagg atctgtattc
     4501 cgagctgatc ttgggaatcg atcccaggct gcgtgaacac caccatccgt tcctgaaggg
     4561 aatgctcgga tttcatgcgg tgctgtcggt gggatacgca attaccggct ttgctgcgat
     4621 tggtcccgcc caaagcgacg taaattccat ggcgcgcgcg ctgcaagttc cgccgtgggc
     4681 gataggaatg atgatccttg ttcccgccgg ctgtgatgtc tacagatact ttgtgcctga
     4741 ctcgcgctgg gccccgtggg taagcctgtc tgcaaagctg acgaccatcg gcgcgatcgc
     4801 cgcgttttga tttgctgccg cgatcgcata gccagcctcg gtagattatc gcgttgtgat
     4861 tcgcgtcacg gcgtgatacg ctctccccga ggtgtcctct gcaatgtccg agcctcggct
     4921 aggtccctat agactgattc gcaagctcag ccaaggtggc atgggcgcag tctatgaggg
     4981 gttccacgag gccgtcgagc gccacgtcgc catcaaggtg ctacgacccg aattcatgag
     5041 caattccgat tttgtgacgc gcttctttaa tgaagccaaa gccgcaaatc ggattggcca
     5101 cccaagcatc gtccagatat tcgattgtgg ccggctcacc gacggaactt cctacatcgt
     5161 gatggagctt ctggatggcg agacggtcgg tatgcggatt cggcgcagtg gtggtcggct
     5221 gccgctgctg ggggcgctgc aagtggtgcg tcaagtcgcc aacgcactcg ccgctgccca
     5281 cgccaaaaac atcgtccacc gagatttgaa accagacaac attatgctgg tgcgtgatcc
     5341 ggcggttccc ggtggggaac gggtcaagct gctcgact
//
LOCUS       DAORGN010000274         5353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7704_length_5353_cov_5.940921, whole genome shotgun sequence.
ACCESSION   DAORGN010000274 DAORGN010000000
VERSION     DAORGN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7704_length_5353_cov_5.940921"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKL17_19990"
     CDS             complement(<1..258)
                     /locus_tag="PKL17_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNK47075.1"
                     /translation="MTAPKHKQQQECTKHRFQDRMIRLIGAAEPSPQTAFDVEKSKCD
                     LVSLSAHKMYGPKGVGALWVRRKPRVRIAPLIDGGGHERGMR"
     gene            214..1947
                     /locus_tag="PKL17_19995"
     CDS             214..1947
                     /locus_tag="PKL17_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47076.1"
                     /translation="MLCALLLLLVFWRSHRLAPQITMRLLLWLALLFAHFFFFEPANP
                     EAWGPSAFALTLLLTIGLCGSGSLPARSLAVCGIAGLAFLLGRSQQESHSQADSVSAF
                     VGAEASSDLPLRELTRFIDINIEPDALLLVDDRLLSSYFHLYSRRSPLVRPYVGIAPA
                     ELRSRYHLTTLSLRFFSPNLSPQDVDARILSGHPVYLLASFATDSESQPLPYHGLHLS
                     RIRQPLYGVQTLPKRPPLETESGAKIDDLLANDLSLLAVTVINPTVYRLTLARGSRQF
                     SAKWKPMGIGGSEQQEGFDGNNAPRCEVAAKIIDRWLADGDSQRELVPDVVLRAFHRN
                     VPCDRSCKDVPRILGEEFAPTFPAWNDHLVIGALTAWIDDGQTPSKFHDGLWNPQRFA
                     REPDYRSSVANLATFLSLIAHGDANYADNFLVREGPPFRVFSIDNGRSLDGIPFYSDD
                     ADPDWEPLRHLAKDQLFLPQVEPRMLARLGQLPQSPLLPALYVVSAVDLIRGKSVADV
                     HAEPRLRPFVDRPLAQQPAAKPIGRGTYLIQDPANSGLWLVQGISAQGVADVAERARA
                     LVKRYSTGKSE"
     gene            complement(1963..2997)
                     /locus_tag="PKL17_20000"
     CDS             complement(1963..2997)
                     /locus_tag="PKL17_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47077.1"
                     /translation="MVSKRPPLLGYNHNVRHAGRLFHVQTEDSGPARPVLTTHLFIEG
                     TILSSQRTPYSIDEPETVTQKRMQDQHKGMLKRLRDGLFDNLPEVQRFPATKADGHGA
                     APTEKAAAGDTQKIGAPKSTPDAAGAKAEASSPIISPVPPPPSAPNVSTSTLAERLKK
                     LNAAAAPAAESKVIIAPDAAADDAVVEIEAAADDDEPALTFEAEADDMMSDTTPLPKA
                     RPLASLDQEDQTAQFGASQMPTAQIPPSRAPAPSVLTSIPPVQRSIPSYTIPPELFKP
                     RPTAEGVTLRRSPPVPIHRAAADPIRTTIVRARPTRPAPLAPTLKPGQAIVDSPLDAE
                     LLGYLSRIAR"
     gene            3146..3844
                     /locus_tag="PKL17_20005"
     CDS             3146..3844
                     /locus_tag="PKL17_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47078.1"
                     /translation="MHRQGDVKADPSRRELHEEPTLYEAEHAASAFGLLLASPEPQDG
                     PSALSAPDARPTTPPAPQAPPAGSVSPPRPNDSWEEATRVPGIGHQATQTLLPVHRPP
                     IEHALPAGAVTEMRQLPGPQASHGPPAGPRQRLLYPFVVGTLGGLLGLLIAMGGLQLV
                     KQRREAQRRERLEILPLQQQVMDRAVRALLAGQRDEARELLQYYELRWPSPGLVQLLQ
                     SIDRLNAQPNGPTR"
     gene            3908..4804
                     /locus_tag="PKL17_20010"
     CDS             3908..4804
                     /locus_tag="PKL17_20010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNK47079.1"
                     /translation="MKSSVRWLFRIVFVAAAVYLFLGFAVVAVLSKPKRHLDASKNPG
                     THGIAYQDVRFASRGGDAELAAWYLPHASHNAALVLVHGKDGSRTHEQNRRNDEFMSA
                     LYRQGFTVLAMDLRGHGQSSPGRFSFGINESRDVLGGFDYLQKQGFDQKHIGLHGVSM
                     GAASVLLAAAQEPAVKAVIADCGYADFGRIMRREWYRRTHLPSLILPSAQVAGQLWLG
                     HDVLSVRPIDRVADIKSQVLFIHAANDTLIPPSDSQDMQAKLPGSALWIQPGARHAAG
                     FDADPTGYVQHVADFFGQTLRN"
     gene            4886..>5353
                     /locus_tag="PKL17_20015"
     CDS             4886..>5353
                     /locus_tag="PKL17_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNK47080.1"
                     /translation="MLSISAPHTPAENLSKASRTLGDMLRRRAELTPSREAFFERRDE
                     RWRPTTWAQAYARACRYAAGLRKLSLTRGDRIAILGPTQLRWTLFDFAAQLLGLVSFG
                     IYQQQTVEQIRYLLAHSEAKVVLVGDEKELANVVAAAQDLPHVVAIVPWDEAVY"
BASE COUNT          914 a         1649 c         1670 g         1120 t
ORIGIN      
        1 ccgcatgccg cgctcgtgac cgccgccgtc gatgagcggc gcgatgcgca cgcggggctt
       61 gcggcgcacc cacagcgcgc ccacgccctt gggcccgtac atcttgtggg ccgacagcga
      121 gaccaggtcg cacttcgact tctcgacgtc gaaagcggtc tgcgggcttg gttctgcggc
      181 ccctatcaaa cgaatcatcc gatcctggaa gcggtgcttt gtgcactcct gctgctgctt
      241 gtgttttggc gcagtcatcg gctggctccg cagatcacca tgcggctcct tctgtggttg
      301 gccctgctgt ttgcacactt ctttttcttt gaacccgcaa atccggaagc atggggtccg
      361 tcggcctttg cgctgacgct gttgctgacg atcggtctgt gcggaagcgg ctctctgccc
      421 gcgcgatcgc tcgcggtgtg cggcatcgcc ggtcttgcct tcctgcttgg tcgcagtcag
      481 caggaatccc attcccaagc cgattccgtt tctgcctttg ttggggccga agccagctct
      541 gatctgccgc tgcgcgagct gacccgcttc atcgacatca acatcgaacc ggacgcgctg
      601 cttctcgtcg acgatcgact ccttagctct tattttcatc tgtacagcag acggtctccg
      661 cttgtgcgtc cgtatgtcgg aatcgctccg gctgagcttc gcagtcgata tcatctgacg
      721 acgctgagtc tgcgcttctt ttcccccaac ctttcgccgc aggatgtcga tgcgcggatt
      781 ctgagtggac atccggtcta tctgctggcc agctttgcga cggattccga atcgcagccg
      841 cttccgtatc acggtcttca tctgtcgcga atcagacagc cgctttatgg tgttcagacc
      901 cttcccaagc ggcctccttt ggaaacagag tccggcgcaa aaatcgatga tctgctggcc
      961 aacgatctgt cgcttctggc ggtcacggtg ataaatccca cggtgtatcg gctgacgctg
     1021 gcgcgcggca gcagacaatt ttccgccaag tggaagccga tgggaatcgg tggttcggaa
     1081 cagcaggaag gttttgatgg caacaatgcg ccgcgctgtg aagtggcggc aaagatcatc
     1141 gatcgctggc ttgccgacgg tgattcccaa cgtgaactgg ttccggacgt ggtgttgcgg
     1201 gcgttccatc gcaacgttcc gtgcgaccga agctgcaaag atgtgccacg gattttgggg
     1261 gaagagtttg cgccgacgtt tccagcctgg aacgatcact tggtgatcgg cgcgctgacg
     1321 gcatggattg atgatggcca aaccccgtcg aagtttcatg acggactgtg gaatccgcag
     1381 cgctttgcaa gggaaccgga ctatcgatcc agcgtggcga accttgcgac gtttttaagt
     1441 cttatcgcgc atggtgacgc aaactacgcg gacaactttc tggttcggga aggccccccc
     1501 tttcgtgtct tcagcatcga caacggaagg tcgctcgatg ggattccgtt ctattcagat
     1561 gatgccgatc cagactggga accgctgcgt caccttgcga aagaccagtt gtttttgccg
     1621 caagtggaac cgcggatgct ggcacggctt gggcagcttc cacaatcgcc gcttctgccg
     1681 gcgctgtacg tggtaagtgc cgtcgatctg atccgtggaa agagcgttgc cgatgtgcat
     1741 gccgaaccgc gcttgcgtcc ttttgtggac cgtccgcttg cgcaacagcc ggcggccaaa
     1801 cccatcgggc gcggaacgta tttgattcag gacccggcga attcgggtct gtggcttgtg
     1861 caaggaatct cggcgcaagg cgtcgccgat gtggccgagc gcgcccgcgc tctggtgaag
     1921 cgatattcga cgggaaaatc ggaataaaca gggcggggca ggctagcggg cgatgcgcga
     1981 cagataacca agcagctcgg catcgagcgg actgtcgacg atcgcctgcc ccggcttgag
     2041 cgtcggagcc agcggcgccg gacgagtcgg acgggctcgc acgatcgtgg ttcggatcgg
     2101 atcggcggca gcgcgatgga tcggaaccgg cggcgaacga cgcagcgtga ccccttccgc
     2161 cgtcggtcgc ggcttgaaaa gctccggcgg aatcgtgtag ctcggaatcg atcgctggac
     2221 cggcggaatc gacgtaagca cagacggtgc aggagcccgc gacggaggga tctgcgccgt
     2281 cggcatttgg ctcgcaccaa actgcgcggt ctgatcttcc tgatcgagcg atgccagagg
     2341 ccgtgctttg ggcagcggcg tggtgtcgct catcatgtcg tcggcttcgg cctcgaacgt
     2401 cagcgcgggt tcatcgtcat cggcagccgc ttcaatttcg accaccgcat cgtcggcagc
     2461 ggcatcgggc gcaatgatga ctttgctttc ggcagccgga gccgccgcgg cattgagctt
     2521 cttgagtcgc tcagccagcg tcgatgtact gacgttcggt gcgctcgggg gcggcggaac
     2581 cggagagatg atcggtgacg atgcttcggc ttttgccccg gcagcgtcgg gcgtcgactt
     2641 cggcgcgccg atcttttggg tgtcgcccgc tgccgctttt tccgtcggag cagcgccatg
     2701 cccatcggcc tttgtcgctg ggaagcgctg cacttcaggc aggttgtcaa acagtccgtc
     2761 gcgcagacgc ttgagcatcc ccttgtgctg atcttgcatc cgcttctgcg tcacagtttc
     2821 cggctcgtca atcgagtagg gagttcgctg cgacgaaagg atggtcccct cgataaagag
     2881 gtgggtggtc aggaccggac gcgccggacc cgagtcctct gtctggacgt gaaacagccg
     2941 tccagcgtga cgaacattgt ggttgtagcc gaggagcggc ggacgctttg acaccaaggg
     3001 ttaggctatc atggctttta gattcactta aagagccaag ttttcgcgtc gctagccgat
     3061 aggggcccga tgtgggcgtc gcggtggctt tcgggcacgt ttttgtgcaa gcacgcgctc
     3121 ggcgctgcgc taaagtcgcg ttttcatgca tcgtcaggga gacgtcaaag ccgacccatc
     3181 gcgtcgcgag ctgcacgaag agcccacgct ctacgaagct gagcatgcgg ccagcgcgtt
     3241 cggtcttttg ctcgcgtcgc ccgagccgca agatggcccg tccgccttgt cggcgccgga
     3301 cgcccgaccg acgactcccc ctgcgccaca ggcaccgcct gctgggtctg tatcaccccc
     3361 gcggccaaac gattcgtggg aagaagcgac gcgggtgcca gggatcggcc accaagcgac
     3421 ccagacgctg ctgcccgtgc accgcccacc cattgagcat gcgctgccgg caggggccgt
     3481 gaccgaaatg cgccagttgc cgggtccgca ggcgagccac gggccgccag ctggtcctcg
     3541 ccagcgcctg ctctatccgt ttgtggtggg cacgctgggt ggccttctgg gccttttgat
     3601 tgcgatggga ggacttcagc ttgtcaaaca gcggcgcgaa gcccagcgcc gtgaacggct
     3661 cgaaattttg ccgctgcagc agcaggtgat ggaccgcgcg gtgcgggcgc ttctggccgg
     3721 acagcgcgac gaagcgcgcg agcttttgca gtactacgag ctgcgctggc catcgccggg
     3781 acttgtgcag ctcttgcagt cgattgatcg gctgaacgcc cagccaaacg ggccgacacg
     3841 ctgattcgtc gcgatccgcg atagagtaaa gctcggacat ttgtccgccc acgaccggag
     3901 tttgccgatg aagtcgtccg tccgctggct ctttcgcatc gtgtttgtcg ccgctgctgt
     3961 ctatcttttt ctcggttttg ccgtcgtcgc cgtgctcagc aaacccaagc gccatctcga
     4021 cgcgagcaaa aatcccggca cacacggcat cgcctatcaa gacgtgcgct ttgcctcccg
     4081 cggtggcgac gcagagcttg cggcctggta cttgccccac gcaagccaca acgccgcact
     4141 tgtgctggtc cacggcaaag acggcagccg cacccacgaa cagaatcgtc gcaacgacga
     4201 gtttatgtcg gcactttatc gacagggctt taccgtgcta gcgatggact tgcgcggtca
     4261 cggtcagtcg tcgccggggc ggttcagctt cggaatcaat gaaagccgcg atgtcctggg
     4321 cggtttcgac tatttgcaaa aacagggctt tgaccaaaaa cacatcggtc tgcacggcgt
     4381 gtcgatgggc gcggcgtcgg tgctgctcgc ggcggcgcaa gaaccagcgg tcaaggcggt
     4441 gattgccgac tgtggctatg ccgattttgg ccgcatcatg cgtcgcgagt ggtatcggcg
     4501 cacccacctt ccgtcgctga tcttgccgtc cgcgcaggtc gcgggacagc tttggcttgg
     4561 ccacgatgtg ctgtcggtgc gtccgattga ccgcgttgcc gacatcaaat cgcaggtcct
     4621 gttcattcat gccgcaaacg acaccctgat tccgccgtcc gattcgcagg acatgcaggc
     4681 gaagctgccc ggatcggcgc tgtggattca gcccggtgct cgtcacgctg ccggcttcga
     4741 tgccgacccc accggatacg tccagcacgt cgccgacttc tttggccaga cgttgcgcaa
     4801 ttagcatccc aaccgctgcc ggctttgcgt aacatcggcg cgctgcttgc ggccttttgc
     4861 caggcccagc gtgggagtct gtccgatgct ctcgatctct gcgccacaca cgcctgctga
     4921 aaacctgtcc aaagcctcgc ggacgctcgg ggacatgctg cgccgtcgcg ccgagcttac
     4981 gccaagccgt gaagcctttt tcgagcgccg cgatgagcgc tggcgtccga ccacctgggc
     5041 ccaggcctat gcccgcgcct gtcgctacgc agcgggacta cgaaagctgt cgctcacccg
     5101 aggggatcgc atcgcaatcc tcggaccgac gcagcttcgc tggaccctgt ttgattttgc
     5161 ggcgcagctg ctcggcctgg tcagtttcgg gatttatcag cagcagaccg tcgaacaaat
     5221 ccgctacctg ctcgcccaca gtgaagccaa ggtggtgctc gtcggcgatg aaaaagaact
     5281 ggccaacgtc gtcgctgcgg cccaagacct gccgcatgtg gtggcgattg tcccgtggga
     5341 tgaagcggtg tat
//
LOCUS       DAORGN010000275         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7867_length_5284_cov_6.582329, whole genome shotgun sequence.
ACCESSION   DAORGN010000275 DAORGN010000000
VERSION     DAORGN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_5284_cov_6.582329"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKL17_20020"
     CDS             <1..500
                     /locus_tag="PKL17_20020"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNK47081.1"
                     /translation="LWKLPVVFIVENNQYSMGTPLERTLAHPDITLRASGFAIERDRF
                     VSDDVLEVRDRIKLAVERARSQGIPSLLEIETYRYRGHSMSDPGNYRTKEEIERWKKR
                     DSIELARTRLLNVLPESEIAAIDAEVREWVAETVRFADSSPPAQESSIWSTVYAQPGT
                     DPRKM"
     gene            502..1200
                     /gene="lipB"
                     /locus_tag="PKL17_20025"
     CDS             502..1200
                     /gene="lipB"
                     /locus_tag="PKL17_20025"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNK47082.1"
                     /translation="MRLFWLGRAPYEPVLRLQQALRREVLSGGAECILLVEHAPVITL
                     GHRATAADLLVSTDRLAAQGIETVRVERGGQATYHGPGQLVVYPICRLRGGVVGHVDW
                     LTAAAVSVADSVGICAEYRQEPVGVWVGEKKIGAVGVHVEHQVAIHGLSLNVTAESTA
                     PFRRGLFVACGARGAQVTSLVEEGAEPSLTTHDVALRFAQTLLRLRGLPPLPVESTRA
                     ELLFDRMASGVARG"
     gene            complement(1159..2283)
                     /locus_tag="PKL17_20030"
     CDS             complement(1159..2283)
                     /locus_tag="PKL17_20030"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNK47083.1"
                     /translation="MIRILELSTENGQQVIREIADERARSLAVTPPPGTVRWVDISKQ
                     TAEELDLIAQGFRFHPLAVEDCLHFDQRPKLEEYAGQAPYLFVVTHNFTILPTTKSLL
                     TDENSLHVPRLLWAESRSQTLALTMLETHAFLGQNFLVTVHDQHSPALEQTWNRVKHD
                     KQFLGRGADFVYYLLADGLCDANFPVLEQIGDILDEMEESLFDNPSRADLHRIYSLRK
                     TLVSMRRVLSPQRDVMGNLARHGGSPCVSEKTAPYFRDIYDHLTRIYESIEAGRDLLG
                     NCVDAYMSAVGQRTNEIMKQLTILSALLLPVTFLSGLFGMNFEMMPFKSPTAFAFALV
                     LMFVVVPGVMFFWFRLKGWLVSDRTDESSPRDTGRHSIKK"
     gene            complement(2361..3266)
                     /locus_tag="PKL17_20035"
     CDS             complement(2361..3266)
                     /locus_tag="PKL17_20035"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNK47084.1"
                     /translation="MKKRVGIIMGGPSAEREVALASGRRVADALTERGHAVVAIDWTG
                     ADHNLWADLRRENVDVAFVALHGTLGEDGCVQGLLECAGIPYTGSGVLASALAMDKVA
                     SRRVFDEASIETPRWTIFEGPADVERIGLPLVVKPSREGSSVGISIVRDEAQLDAALR
                     LARRLHGVVLLEEFVAGREIEVAVLDGEVIGEVEVRPAGEFYDYAAKYQRTDTKYLVP
                     APLSPAERQLCHDLARRAHSALGCSGVTRTDLLLSPTGRAVCLEVDTLPGLTDRSLVP
                     KIAEHAGLSFGVLCERILATAALKA"
     gene            complement(3263..4201)
                     /gene="murB"
                     /locus_tag="PKL17_20040"
     CDS             complement(3263..4201)
                     /gene="murB"
                     /locus_tag="PKL17_20040"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNK47085.1"
                     /translation="MPLSSSQRQTLLDLVGEKGEVRFDEPMARHSTLRVGGPADAWVA
                     PGNIDRLAAIRRFCSESGLACRALGSGSNILVQDGGIRGVVLATERLRGLQFFDPAPD
                     DPRTPVFVEAGVSTGKLLSEATRRSLGGVEFLGGVPGSVGGGMIMNAGTYLGEFKDVT
                     VEVLSIAADGAEIRRSHAECGFVYRGSALPASEVVVAARLLLPQRPQEEIAQAVAALR
                     TRRKEREPHGVGCAGSFFKNPPGDYAGRLIEACGLKGTRRGDAEISTVHANWFVNVGS
                     ARAADLVSLIELSRSHVEQRFGITLDLEVKILGEPA"
     gene            4440..5093
                     /locus_tag="PKL17_20045"
     CDS             4440..5093
                     /locus_tag="PKL17_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HNK47086.1"
                     /translation="MRWAELETAVERNAPDTESFLDTRSGEVITLVESAPNVDELRTK
                     VMSGGDAYLRVEPASSREQYKWMERFVAGVTEPVLRERLIISIDGKGAFRRFKDALLN
                     YPVERERWFAYRGELLHWHIQEWFKRDGLTTEEPPPWGVVPQPVETEVQLPRPSPTTE
                     APGDGYRRQARDLLEQLPAAELPAAIAFLEFLKERGSGELSRSRPASGRPKTGNAGV"
     gene            5094..>5284
                     /locus_tag="PKL17_20050"
     CDS             5094..>5284
                     /locus_tag="PKL17_20050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47087.1"
                     /translation="MAPVGFILKRDQPQSVQLCEQLVQVLRERGQTELVLRDHVQLPS
                     WFHTTSETDLPSELAMLVAL"
BASE COUNT         1024 a         1578 c         1660 g         1022 t
ORIGIN      
        1 gtctgtggaa gctgccggtg gtgtttatcg tcgaaaacaa ccagtattcg atgggcacgc
       61 cgctggagcg cacgctggcg catccggaca tcacgctgcg ggcgtcgggt tttgcgattg
      121 agcgcgaccg ctttgtatcg gacgatgtgc tcgaagtgcg cgatcgcata aagcttgccg
      181 tcgagcgcgc acgcagccag ggaattccgt cgctgctcga aatcgagacc taccgctatc
      241 ggggtcactc gatgtctgat ccgggcaact accggaccaa agaagagatc gagcgctgga
      301 aaaaacgcga ctcgattgag ctggcgcgga cgcggcttct gaatgttctg cccgagtctg
      361 aaatcgccgc aatcgacgct gaagtacgcg aatgggtggc cgaaacggtg cgctttgccg
      421 attcgtcacc gcccgcgcaa gaatcgagca tctggtcgac ggtgtacgcg cagcccggaa
      481 ccgatccgcg aaagatgtag catgcggctt ttttggctgg gtcgcgcgcc gtatgagccg
      541 gtgctcaggc tccagcaggc gctgcgtcgc gaagtcctgt ccggcggggc ggagtgtatc
      601 ctgttggttg agcacgcccc ggtgattacg ctggggcatc gggcgacggc ggctgacctt
      661 ttggtatcga cggatcggct ggctgcgcag ggcatcgaaa ccgtgcgtgt cgagcgtggt
      721 ggccaagcga cctatcatgg accgggtcag ctcgtggtgt atccgatctg tcggctccgc
      781 ggtggtgtcg tcgggcatgt cgattggttg accgcggcgg cggtgtcggt ggccgattct
      841 gtggggattt gtgccgaata tcgccaagag cccgtcgggg tgtgggtcgg tgaaaaaaaa
      901 atcggagccg tcggtgtcca cgtcgaacat caggtcgcga tccacggact gtcgctgaat
      961 gtgactgccg aatcgacggc gccgtttcgc agaggcctgt tcgttgcgtg tggcgcgcgt
     1021 ggggcacagg tgacaagtct tgtcgaagaa ggcgcagaac cgtcgctgac gacccacgac
     1081 gtggcgctgc gctttgcgca gacactgctg cgcctgcgcg gtctgccacc acttcccgtc
     1141 gagtcgacgc gcgctgagct actttttgat cgaatggcgt ccggtgtcgc gcggggatga
     1201 ttcgtcggtc ctgtcgctga ccagccagcc tttcagacgg aaccagaaga acatcacgcc
     1261 cggcacgacg acgaacatca gcaccagcgc gaaagcgaat gccgtcggag atttgaacgg
     1321 catcatttcg aagttcatcc caaaaagacc agacaagaac gtgacgggca gaagcaacgc
     1381 cgacaagatt gtcagctgct tcatgatttc gtttgtgcgc tgtccgacgg cagacatata
     1441 ggcatcgacg cagttgccga gcagatcgcg accggcttcg atgctttcgt agatgcgcgt
     1501 caggtgatcg taaatgtcgc gaaagtaggg cgccgtcttt tcgctgacgc aggggctgcc
     1561 gccgtgccgt gccaggttgc ccatcacatc tcgctgtggc gacagcaccc gtcgcatcga
     1621 caccagcgtt ttgcgcagcg aatagatgcg gtgcagatcg gcgcggctcg gattgtcgaa
     1681 cagcgattcc tccatttcgt cgagaatgtc gccgatctgt tcgagcaccg ggaagtttgc
     1741 gtcacaaagc ccgtcggcca gcagatagta gacaaagtcg gcgccgcgcc cgagaaactg
     1801 cttgtcgtgc ttgacgcggt tccacgtctg ctcaagtgcc ggactgtgtt gatcgtggac
     1861 ggtcacaagg aagttttgcc cgagaaaggc atgcgtttcg agcatcgtca gcgcgagcgt
     1921 ctgcgaccga ctctccgccc acaaaagccg tggtacgtgc agcgagtttt catcggtcaa
     1981 aaggctcttt gtcgtcggca aaatcgtgaa gttgtgggtc acaacgaaca gatacggagc
     2041 ctgcccggcg tactcttcca gcttgggacg ctgatcaaag tgcaggcagt cctcgacggc
     2101 aagcgggtgg aagcggaagc cttgggcgat cagatccagc tcttcggcgg tctgtttgga
     2161 aatatcgacc caacgcaccg tccccggcgg tggcgtcacg gcgagcgaac gcgctcgctc
     2221 gtcggcgatt tcgcggatga cctgctgtcc attttccgtc gagagttcca aaatccgaat
     2281 catcgacgct tccccgtgtg acggtaaaaa gggtatcgca aacggctccc gtttggggaa
     2341 cgctttcgac gagaaatgcg ctaggccttg agcgctgcgg tcgccaagat gcgctcgcag
     2401 agcacaccaa aagaaagacc ggcgtgttcg gcgatttttg gcaccaggct gcgatcggtc
     2461 agtcctggca gcgtatccac ttccaaacag accgcacgcc ccgtcggtga aagcagcaaa
     2521 tcggttcggg taacgcccga acagccaagc gccgagtggg ctcgacgggc cagatcgtga
     2581 cacagctgcc gttccgccgg acttagcggc gccgggacca agtatttggt gtcggtgcgc
     2641 tgatacttcg cggcatagtc gtagaattcc ccggcgggtc gaacctcgac ttcaccgatg
     2701 acttcaccgt cgagaaccgc gacttcgatt tcacgcccag cgacaaactc ttcgagcagc
     2761 accacgccat gcagacgccg tgcgagccgc agcgccgcat caagctgcgc ttcatcgcgc
     2821 acaatcgaaa tgcccaccga cgagccttcg cgcgaaggct tcaccaccag cgggagtccg
     2881 atgcgctcga cgtcggcggg cccttcgaaa atcgtccagc gcggcgtttc aatgctggct
     2941 tcatcgaaca ctcgacggga cgcgactttg tccatcgcca gcgcggatgc cagcacgccc
     3001 gatccggtgt acggaatgcc ggcgcactcc aaaagcccct ggacgcagcc gtcttcgccg
     3061 agcgtgccat gcagtgccac gaacgccacg tcgacatttt cccgtcgcag atcggcccac
     3121 aggttgtgat cggcgcccgt ccagtcaatc gcgaccaccg catgcccacg ctcggtcagt
     3181 gcgtccgcca cccgtcgccc tgacgccagc gcgacttcgc gttccgccga cggcccaccc
     3241 atgatgatgc ctacccgttt tttcatgcgg gctctcccaa aattttgact tccaaatcga
     3301 gcgtgatgcc gaagcgctgc tcgacgtgac tgcgcgaaag ttcgataaga gacaccaaat
     3361 cggcggcccg cgcgctgccg acgttgacga accagttggc gtggaccgtg gaaatttcgg
     3421 cgtcgccgcg acgggttcct ttgagtccgc aggcctcgat gagtcgtccc gcgtagtcgc
     3481 ccggtgggtt tttgaaaaaa ctgccagcac aaccgacgcc gtgcggctcg cgttcttttc
     3541 gtcgcgttcg cagcgctgcc acggcttgcg cgatttcttc ttgcggtcgc tggggcaaaa
     3601 gcaggcgagc cgcgacgacg acttcgctgg ctggtaaggc cgagccgcga tagacaaagc
     3661 cgcactcggc gtggctgcga cgaatctctg caccgtctgc cgcaatgctc aacacctcga
     3721 cggtgacatc tttgaattca ccgaggtagg tgccggcatt catgatcatc ccgccgccaa
     3781 cagaaccggg gacaccaccg aggaattcga cgcctccgag gctgcgccgg gtcgcttcgc
     3841 tgagcagctt gccggtgctg acgcccgctt caacgaaaac cggtgtgcgt ggatcgtcag
     3901 gcgcggggtc gaagaactga agaccacgca gccgttccgt cgccaagacc acgccgcgga
     3961 tgccgccgtc ttgcaccaaa atgttcgatc ccgatccgag cgcgcgacag gcaagaccgg
     4021 attcggaaca gaagcgacgt atcgccgcca gacggtcgat gttgcccggc gcgacccatg
     4081 catcagcggg gccaccgacg cgcagggttg agtgacgcgc catcggttcg tcgaagcgca
     4141 cttcgccctt ttcaccgacc agatcgagga gggtctgtct ttggctcgac gaaagcggca
     4201 tgatgctggc gttctagcct gaccgctcgc tgctgccaat ttttccgccg tttgaacccg
     4261 agcacgaagt ggccgtagcc cttgcaaatc tccgagcaga tccatagcct ttaagatcac
     4321 attaggaact tggctgtgct tggtcttcgt cggcaaatcg agaagcagtc tgcgcaggcc
     4381 cagctacata tgctgtatct cttaccgaat ggggtttgtt ggcgatgcga gtgatagcgg
     4441 tgcgttgggc ggagttggaa acggcggtcg agcgcaatgc gccagacacc gagagcttcc
     4501 tcgatacccg ctcgggtgag gtgattactc tcgtcgagtc tgcacccaat gtcgacgagc
     4561 tgcgtaccaa agtgatgagc ggcggggatg cctatctgcg cgtcgagccg gcgtcctcgc
     4621 gagagcagta caagtggatg gaacgcttcg tcgcgggagt gacagagccc gtgctccgtg
     4681 agcgtttgat catcagcatc gacggaaaag gtgcatttcg gcgttttaaa gacgcactgc
     4741 tcaactatcc cgtcgagaga gagcgctggt tcgcctaccg cggcgagctg cttcattggc
     4801 acatacaaga gtggtttaag cgcgacggct tgacgacgga ggagccgccg ccatggggcg
     4861 ttgtgccgca gcctgtggaa accgaagttc agctgccgcg gccaagtccg acgacggaag
     4921 caccgggtga tggttatcgt cgacaagcgc gagacctgct cgaacagctt ccggcggcgg
     4981 aactgcctgc cgctatcgcg ttcctcgagt ttttgaaaga gcgcggcagt ggcgagctgt
     5041 cgcggagtcg tccggcgtcg ggacggccca agaccgggaa cgcaggagtt tagttggctc
     5101 ccgtcggttt cattctcaag cgcgatcagc cgcagtcggt gcagttgtgc gagcagctcg
     5161 tgcaggtgct gcgcgagcgc ggccagacgg aactcgtgct gcgcgatcac gttcagctgc
     5221 ccagctggtt ccatacgacg agcgagaccg atctaccgag tgagctggcg atgctggttg
     5281 cgct
//
LOCUS       DAORGN010000276         5267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7913_length_5267_cov_6.646009, whole genome shotgun sequence.
ACCESSION   DAORGN010000276 DAORGN010000000
VERSION     DAORGN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7913_length_5267_cov_6.646009"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKL17_20055"
     CDS             complement(<1..585)
                     /locus_tag="PKL17_20055"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNK47088.1"
                     /translation="MLDSVRQNSRSAIIYILFGILIAAFIVSFGPGSPTGDSVFDGLG
                     GHYAARVYGKDISEPELNFSFIALGLPNRGEAQAQKLREVVIDRLIERELLAHEAEQA
                     GLLVSEDEAAKLLVDGNMFVAGIARKVEPYAMRNNVLDYERLRMVVQNSYRLTMKQFV
                     DIQRRELLADKFRQLLRAGTRASLDEIRGEYEDKN"
     gene            complement(669..1484)
                     /locus_tag="PKL17_20060"
     CDS             complement(669..1484)
                     /locus_tag="PKL17_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47089.1"
                     /translation="MMRQRASVSRWATLAAALFVTSVGGCGVGSSDDGVAQLSEAVCV
                     PGDKGGDVGCGCSLNNQCSGFDDETRLLICDVASGSSVGKCSDCLSLTARPTGCACNV
                     DANCATGLVCNGRTCQALRKRGEFCVRDTDCGSDATGAMVCLPSKNWCGPLAVGSFCD
                     FNLDCLSGICSLGKCSTGESGQPCSVDKNCKAGLVCNTVAGTCGAKLADGQPCLRNVE
                     CQNQCNSFSGTCKLGSVGTVCTTANPDGDCGSGLSCVDCGGGVKTCRTGGSCT"
     gene            1717..2955
                     /locus_tag="PKL17_20065"
     CDS             1717..2955
                     /locus_tag="PKL17_20065"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNK47090.1"
                     /translation="MRKTLPSVVGSLLVLQLWPQIAGAAVCKVPGTHPTIQKAVDDTS
                     CTDIQLEAQTYTEQVSIGRAGLTITGKGAGRTILRSPNSRVRSTVSTTYLPRYTYVVE
                     IKPGALASLRDLSIDGRSNATCTERFFGVRYHNSTGNLERIVVENVRGQGANFAASCQ
                     NIIGVGVTSDGSGGGSVQLTQSTIRNFQAVGVLASGTNAGVVVQNTVVRGAGPQSNLP
                     QTGIRFTNGAAGAADRNNLFDLRFTGDPCRGVGTGLDFYRAGQAFITTNVVVDADRGL
                     WLRENQNPQVAYDNRFIRNYLGIESSQNSDGQVRISKNAFSELKRSTAATVDSCFAFS
                     GDGIAVEDELDTVVIQNSIADSARNAIELGAGTENLDVQQNQSVRSSKSDIDDQGTAN
                     RLTLNRCSKSAPTGLCSDTP"
     gene            complement(3015..4694)
                     /locus_tag="PKL17_20070"
     CDS             complement(3015..4694)
                     /locus_tag="PKL17_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47091.1"
                     /translation="MHKLGSSALVTVFCGLGLSGCYQGEWRLGTEVPVSVTTRLHPVR
                     TVETWELSEPGKNAVVSLRATSTQRCRQAMYGKTSRTDTGTFERLGGNWWKGAAIVTG
                     IAGGAGVGIGLGGTIAHLDPKYGGPITYATGGAVAAGGIASCLASISNGSKVRYAFCG
                     ILTGLGASILAGGLLSIIPGSSTGTGTGTGTGSGSSATTMTSSSLVDASTFQTILIGG
                     GALVGTSILTGIIGQAWQGNEDRIRTVDSSSTTMWDDQQPESACSAPSLLQARSAMLN
                     IFVDTIPSGPGSAEKPLKVKVQPVGPGAQMVDLSAIRQTLPNCGALTVKVVPDVVYPA
                     FSDDYTPVVGPDFISRAVRPLFTQILPSDGVSLESAATPVKSTQPTKAMLHGYSLETL
                     RQIDRVCSGETAPQPKPPAHHPIVVLQLPPTETPTDPAKVVTAPKTEPAVEEPILTPG
                     LQVVQPSEGHEQDAECSTLAQKTRLHDCELQCGRNWQVSSCLAEFRPCLALSKTASQP
                     LRERALCETNWYDCIEKKGVASSTFRSCTEACADANTPSLCRERKTQRLSP"
     gene            complement(4695..>5267)
                     /locus_tag="PKL17_20075"
     CDS             complement(4695..>5267)
                     /locus_tag="PKL17_20075"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47092.1"
                     /translation="HCPEEKGPAENNGCPERDTDRDGIVDRLDDCPTLPGRKALHGCP
                     RVYADQNKIKVLERIHFATDQDVILPESFGVLEEIADYIKAHPEWLEILIEGHCDFRA
                     DDAYNLNLSQRRASAVLKFLLLRGVEPGRLKAQGFGKSRPIADNSTEEGMALNRRVEF
                     TILKVAPGHQLPVLERPDQVKIEPVLRSQN"
BASE COUNT         1031 a         1693 c         1550 g          993 t
ORIGIN      
        1 gttcttgtct tcgtactcac cgcggatctc gtcgagagaa gcccgcgtgc cggcccgcag
       61 caactggcgg aacttgtcgg cgagcagctc acgacgctgg atgtccacga actgcttcat
      121 cgtcagccga tacgagttct gcaccaccat gcgcagccgc tcgtagtcga ggacgttgtt
      181 gcgcatcgca tacggttcga ccttgcgagc gatcccggcg acgaacatgt tgccgtcgac
      241 gagtagcttc gcggcttcat cttcggacac cagaagaccg gcctgctctg cctcgtgggc
      301 cagaagctca cgctcgatca ggcgatcaat cacgacctcg cgcagcttct gcgcctgggc
      361 ttcaccgcgg tttggcagac ccagcgcgat gaacgaaaag ttcagctccg gctcactgat
      421 gtctttgccg tagacccgcg ccgcgtagtg accgcctagc ccatcaaaca ccgagtcccc
      481 cgtcggagat cccggaccaa agctgacgat gaaggcggca atcaggatgc cgaagaggat
      541 gtagatgatg gcagagcgag agttctgccg caccgaatcg agcatgaagc gcgtgctcct
      601 ttaacgaata acgctggggt cgagactcta cccgagggat gccaagccag caacaaaacg
      661 ggccgacttt acgtgcaaga tccaccggtc cggcaggtct tgacgccgcc gccgcaatcg
      721 acgcagctta gacccgagcc gcagtcaccg tcgggattcg cagtggtgca aacggttccc
      781 accgatccaa gcttgcaggt gccgctaaag ctgttgcact ggttttggca ttcgacgttg
      841 cgcagacacg gttgaccatc ggcaagcttt gcgccacagg ttccagcgac ggtgttgcaa
      901 accaagccag ctttgcagtt tttgtcgacg gagcacggct gaccgctttc ccccgtcgag
      961 cacttgccca aactgcagat cccgctcagg cagtcgaggt tgaaatcaca gaacgatccg
     1021 acggccagcg gtccacacca gtttttgctg ggcagacaca ccatcgcgcc ggttgcgtcg
     1081 ctgccacagt cggtgtcgcg cacgcaaaac tcgccgcgct tgcgcagcgc ctggcaggtc
     1141 cgtccgttgc agacaagccc ggtggcgcag tttgcgtcca cattgcaggc gcagcccgtc
     1201 gggcgagccg tcaagctcaa gcagtccgag cacttgccga cgctgctgcc gctcgcgaca
     1261 tcgcagatga gcaggcgcgt ttcgtcgtca aagccgctgc actggttgtt cagcgagcag
     1321 ccacagccga cgtcgccgcc tttgtcgccg gggacgcaga ccgcttcgct aagctgagcg
     1381 acgccgtcat cgctcgatcc gacgccacag ccgccgacgc tggtcacaaa gagcgctgcc
     1441 gcaagagtcg cccacctact gacactggcc cgctgtctca tcatgaccgt ctcctcattg
     1501 cttgatttga agcacgttgg ctgtgcttcc ttcgccactt ttgcacggtt ttgagaagtt
     1561 cgcatcccga atggcgatcg gttttttgtg cagcttacct tgccttcggt ttgggggaag
     1621 atccgaaaaa gtgctaagaa cctgagcaga attcagggtc tgcgtgcgga cgcaaaagtg
     1681 ttgcccgcgc aaggaccggc ttcgaggaga cagcctatgc gaaagacgct cccgtcggtt
     1741 gtgggttcgc ttttggtact tcagctttgg ccgcagatcg ctggcgctgc cgtctgcaaa
     1801 gtcccaggta cgcatccgac catccaaaag gccgtcgacg acaccagttg taccgacatc
     1861 cagctcgagg cccagaccta caccgagcaa gtctcgattg gccgcgccgg tctcaccatc
     1921 accggcaagg gcgccggtcg caccatcctg cgcagcccaa acagccgggt tcgctcgacg
     1981 gtgagcacaa cctatctgcc gcgttacacc tacgtcgttg aaataaagcc cggcgcgctc
     2041 gccagcttgc gcgatctgag catcgacgga cgcagcaacg ccacttgcac ggaacgattc
     2101 ttcggcgtcc gctaccacaa ctcgacggga aatttggaac ggatcgtcgt cgaaaacgtc
     2161 cgcggccagg gggcaaactt tgccgcgtcg tgccaaaaca tcatcggcgt cggcgtcacc
     2221 agtgacggca gcggtggcgg atcggtccag ctgacccagt cgaccattcg caatttccaa
     2281 gccgtcggcg tactcgcgag cggcacaaat gccggcgtcg ttgttcaaaa caccgtcgtg
     2341 cgcggtgccg gtccgcaaag caacctgccg cagaccggca ttcgcttcac aaacggcgcc
     2401 gccggagccg ccgatcgcaa caacttgttt gatctgcgct tcaccggcga cccctgtcgt
     2461 ggcgtcggca ctggtcttga tttttaccgc gccggccagg ccttcatcac caccaacgtg
     2521 gtcgtcgacg cggatcgcgg cttgtggctg cgcgaaaacc agaatcccca ggtggcctac
     2581 gacaaccgct tcatccgaaa ctatctcggg attgaaagca gccagaacag tgacggccag
     2641 gtccgcatca gcaaaaacgc cttttccgag ctaaagcgca gcacggcggc caccgtcgat
     2701 agctgcttcg cgtttagtgg cgatggaatt gccgtcgaag acgagctgga cactgtggtg
     2761 atccaaaaca gcatcgccga cagtgcacgc aacgccatcg agctcggtgc cgggacggaa
     2821 aatctcgatg tgcagcaaaa ccagagcgtg cgctcaagca aaagcgacat cgacgatcaa
     2881 ggcacggcca accggctcac gctcaatcgc tgtagcaaga gcgcgccgac gggactctgc
     2941 agcgacacac cgtaaaccga cgttggctgg gaccgattgc ccgccatcgc agcgaagcga
     3001 aaattccccc cggcctaagg cgacagccgc tgtgtcttgc gctcgcggca caaggacggg
     3061 gtgttggcgt cggcgcaggc ttcggtgcac gaacggaacg tgctgctcgc cacgcctttc
     3121 ttttcgatgc agtcgtacca gttcgtttcg cacagcgcgc gttcccgcag cggctggctc
     3181 gcggtctttg acagcgccag acacggacgg aattcggcca gacagctgct gacctgccag
     3241 ttgcgcccac actggagctc gcagtcgtgg agccgcgtct tctgcgccaa cgtgctgcac
     3301 tcggcgtctt gttcgtgacc ttcgctgggc tgcacaacct gcaaacccgg cgtcaggatc
     3361 ggttcctcga cggcaggttc ggtcttcggc gcggtgacga ccttggccgg atcggtcggc
     3421 gtttccgtcg gtggaagctg gagcacgaca atcggatgat gcgcgggcgg cttcggttgc
     3481 ggggctgtct cgccactaca gacgcgatcg atttgtcgca gcgtctctaa cgagtaccca
     3541 tgcagcatcg ctttggtagg ctgcgtactc ttcactgggg tggcggcgga ttccagcgac
     3601 acaccgtcgg acggaaggat ctgcgtaaac agcggacgca ccgctctcga gatgaagtcg
     3661 ggaccaacca ccggcgtgta atcgtcggaa aacgccggat agaccacgtc aggaaccacc
     3721 ttgaccgtca gggcaccgca gtttggcaag gtctgccgaa tcgcgctcag atcgaccatc
     3781 tgcgcaccgg gaccgacggg ctgcactttg actttgagcg gcttttcggc gctaccggga
     3841 ccgcttggga tggtatcgac gaaaatgttc agcatggcac tgcgcgcttg cagtagcgac
     3901 ggggcgctac aggccgactc gggttgctga tcgtcccaca tcgtcgtgct cgaactgtcg
     3961 acggtgcgaa tgcggtcttc gttgccctgc caggcctgac cgatgatgcc cgtcagaatc
     4021 gacgtaccga cgagagcgcc acccccaatg aggatggttt gaaaggtcga tgcatccacc
     4081 agcgacgagc tggtcatcgt cgtcgcgctg ctgccactgc ccgtaccggt gcccgttcca
     4141 gttcccgtcg atgatcctgg gatgatggat aacaaaccgc ccgcgagaat cgacgcgcca
     4201 aggccggtca gaatgccgca gaaagcgtag cgaaccttgc tgccgttgga aatcgacgcc
     4261 aggcagctgg caataccgcc cgcggcaacc gctccgcccg tcgcatacgt gatcgggcca
     4321 ccatactttg gatcaagatg cgcgatcgtg ccgccaaggc caatgccgac gccggcaccg
     4381 cccgcaatgc cagtgacgat cgctgcgccc ttccaccagt tgccgccaag tcgttcgaat
     4441 gttccggtgt cggtgcggct ggtcttgccg tacatcgcct gccgacagcg ctgcgtcgac
     4501 gtcgcacgca gcgaaacgac cgcgtttttc cccggctcgc taagctccca cgtctcgacg
     4561 gtgcgcaccg gatgcagccg cgtcgtgacc gagacaggga cttccgtccc aagccgccac
     4621 tcgccctgat agcaacccga aagcccgagt ccgcaaaaga ccgtgacgag cgcagacgag
     4681 cccagcttgt gcatctaatt ctgactccgc agcactggct caattttgac ctgatcaggc
     4741 ctttccagca cgggaagctg atggcccggc gcgactttca gaatcgtaaa ctcgacgcga
     4801 cggttgagcg ccatgccttc ttcggtgctg ttgtccgcga tcggacggct cttgccaaag
     4861 ccctgcgctt tgagccgccc aggctcgacg ccgcgaagca gcaagaactt gagcactgca
     4921 cttgcccgcc tctgcgacaa gttcaagttg tatgcatcgt cggcacggaa gtcgcagtga
     4981 ccctcgatca ggatctccag ccactcgggg tgcgccttga tgtagtcggc aatttcttcg
     5041 agcacaccaa atgactcggg gaggatcacg tcctggtcgg ttgcaaagtg aatccgctcg
     5101 agcaccttga tcttgttttg atcggcgtag acgcgcgggc aaccgtgcag cgctttgcgt
     5161 cctggcagcg tcgggcagtc atccagtcga tcgacgatgc catcgcgatc ggtgtctcgc
     5221 tcggggcagc cgttgttttc cgccggacct ttttcttcgg gacagtg
//
LOCUS       DAORGN010000277         5265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_7918_length_5265_cov_6.052591, whole genome shotgun sequence.
ACCESSION   DAORGN010000277 DAORGN010000000
VERSION     DAORGN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7918_length_5265_cov_6.052591"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKL17_20080"
     CDS             <1..508
                     /locus_tag="PKL17_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase of SIR2
                     family"
                     /protein_id="HNK47093.1"
                     /translation="FTPQGDYCRYQCLTPCTQETWSTESLFATFLPLVDKRTQQLPRG
                     MHPKCPRCGGPTFLNVRGGDWFVETPYEEESARHAEWLAKHVKKRLLVLDLGSGFNTP
                     MWIRWPAENLVAEHPNARLVRVNLHNPEVPSAIADRAVSFASGADVVIDAVLRERLLR
                     ARSDSATL"
     gene            521..1081
                     /locus_tag="PKL17_20085"
     CDS             521..1081
                     /locus_tag="PKL17_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47094.1"
                     /translation="MSSSHEVDSVLPDEITKLLADPSLPEPIANALRAGMKLERIELD
                     AEGCFWHQGERFQNDKLSSLFHRSIQRTDGGTYLIVIPPYSYPFVVADAPRQVRHIRQ
                     DDARVWLVLSDEQEQILDPSTLSYVPDRGLYCQVDAPGVQRWPARFLRPAYYALLSHI
                     DQQGEDYFLDLPGGRWPIPVVSQPPR"
     gene            1274..1903
                     /locus_tag="PKL17_20090"
     CDS             1274..1903
                     /locus_tag="PKL17_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47095.1"
                     /translation="MRSFSAMFLVLATAATGCTHFHTNMLSESIKAFSAKAKPANQKV
                     WVASVADSNACDSGCQLPGSCGKSSSPLGGKGLFGGMGGGGSDKLAYEVITNYLTQNQ
                     TMKVVESHRHNYATDTRVETRKKLDVAMEGKAVASSMSCEDLCLLDEAKKRNADKVLT
                     YHIIDMGAKKLTIHYRLSDVKSGTVDASYTLHVSHPQQAGDVSFVEPAP"
     gene            complement(1913..2785)
                     /locus_tag="PKL17_20095"
     CDS             complement(1913..2785)
                     /locus_tag="PKL17_20095"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNK47096.1"
                     /translation="MTRPIENAAATENIRLRLVTLNLWGEQGPFEKRFPLCQKELRAL
                     DADVVALQEVREIPGTLRNTAEQLAEHLGMSFVFAPASAWGGGTEGLAILSRLPIAAH
                     VVHELPNERPEERRVLLIADIQTQVGTVIAATTHLHYRMTDGIAREQQVEAIDRFLKA
                     HAQSVSGGAKLTLLAGDFNATPDSDELRFLRGLHTLGGRRTYYQDAFLCATDDPKGPG
                     YTWSRRNPYTQRLRFLQNDRRLDYIFVSPASRDGRGTVTDCRIVLDRADTDGVYPSDH
                     FGLCAEVLLSPLPY"
     gene            complement(2782..3168)
                     /gene="queD"
                     /locus_tag="PKL17_20100"
     CDS             complement(2782..3168)
                     /gene="queD"
                     /locus_tag="PKL17_20100"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNK47097.1"
                     /translation="MYVELVREYRFESAHFLPKVPDNHRCRRMHGHSFKIDVVLGGEA
                     NEETGFLIDFYDLDRVVQPVIDSIDHRLLNEIPGLENPTSEVIARYVFDRIFPSLPGL
                     CSVTLWETVDSRCIYYGPHGPVRNRG"
     gene            complement(3194..4066)
                     /locus_tag="PKL17_20105"
     CDS             complement(3194..4066)
                     /locus_tag="PKL17_20105"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNK47098.1"
                     /translation="MALIPYRDVPETENLKKDPPFSVAGQPVEEFYCGDHACDCATAH
                     LRVGGVVMTVDLGTQRVDFLDNQNAPGREILRKGVRDAIQSGQLDNLRKHYTDTREFG
                     KTLHFRYFDWSGVKPGEMVLWQHVFRAEGIPLFTMTSDEKQPAAEGEEPKPAPQVSIG
                     LQDAYCIEPKCDCKRVVWTVVSPGPGGAAQKIGVVNFSLPKKEPAVLEVTPGVDANSL
                     FVLVSNLLSSQPQLVKMYENRYRFMRDQVSPLLASQRQLRNQSKQRPQTAVGRNDACP
                     CGSGKKYKKCHGAN"
     gene            complement(4120..4800)
                     /gene="gmk"
                     /locus_tag="PKL17_20110"
     CDS             complement(4120..4800)
                     /gene="gmk"
                     /locus_tag="PKL17_20110"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNK47099.1"
                     /translation="MTQAGTGLLLVVSSPSGAGKTTLCKRLLAERPELRFSVSYTTRP
                     PRSGERDGVDYHFVSDATFDDMVSRGAFAEWNWVHGRRYGTGIETVRQALASGCKVLL
                     DIDYQGATRILAEFADAARLIFILPPSLPILEARLRGRGTDSEEVIATRLRKARDELS
                     HFSMYHYLVLNRDLSVGYDELRAVYLRELAKAEGRAPSDDEEACAAACLTTNRAAVAE
                     RILAGGEA"
     gene            complement(4804..5112)
                     /locus_tag="PKL17_20115"
     CDS             complement(4804..5112)
                     /locus_tag="PKL17_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNK47100.1"
                     /translation="MAVRLVNIGYGNAVVAERVVAIVSPESAPMKRVKEEARKAGRLI
                     DGTQGRRTRSILITDSDHIVLSALQTETVAERLERGGIGGASSSALGADGEASARDEA
                     "
     gene            complement(5158..>5265)
                     /locus_tag="PKL17_20120"
     CDS             complement(5158..>5265)
                     /locus_tag="PKL17_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNK47101.1"
                     /translation="ANTLTSKAQSAEVAARVVAMKAELERLREQVQNVE"
BASE COUNT         1015 a         1592 c         1573 g         1085 t
ORIGIN      
        1 ttttactccg caaggggact actgccgcta ccagtgtctt actccatgca cgcaggaaac
       61 gtggtctaca gagtcgctgt ttgcgacgtt tctgccgctc gttgacaagc ggacacagca
      121 gcttccgcgc gggatgcatc cgaagtgtcc gcgctgtggc ggtccgacat tcctgaatgt
      181 gcgcggtggc gactggtttg tcgaaacccc ctatgaagag gagtcggcgc gccatgcgga
      241 atggctggcc aagcatgtga aaaagcggct gctggtgctc gatctggggt cgggatttaa
      301 caccccgatg tggattcgct ggccagcgga aaatctggtg gcggaacacc caaatgcgcg
      361 gcttgtgcgg gtgaacttgc acaatccgga agttcccagc gcgattgcgg atcgcgcggt
      421 gagctttgcc agtggcgcgg acgtggtgat cgacgcggtg ctgcgcgagc ggcttctgcg
      481 ggcacgatca gacagcgcga cactctgata gtctcggcgt gtgtcatcgt cacatgaagt
      541 cgactccgtt ttaccggacg aaattaccaa gctgctcgcc gatccgagcc tgccggaacc
      601 catcgccaac gcgctgcgcg ccggaatgaa actggaacgc atcgagctgg atgccgaagg
      661 ttgtttttgg caccagggcg agcgctttca aaatgacaag ctaagctccc tgtttcatcg
      721 ctcgattcag cgcaccgacg gtggaaccta tctgattgtg attccgccct attcctatcc
      781 gtttgtggtg gccgacgcgc cgcggcaggt ccggcacatt cggcaagatg acgcgcgggt
      841 gtggcttgtg ctttcggatg aacaagagca gatcctggac ccgtcgacgc tcagctacgt
      901 gccggatcgc ggactgtact gccaggtcga tgcacccggt gtccagcgct ggcccgcgcg
      961 ttttctgcgc ccagcgtact acgcgctgct ttcgcacatc gaccagcagg gggaagacta
     1021 ttttctcgat ttgccggggg ggcgctggcc gataccggtc gtttcgcagc cgccgcgctg
     1081 aatcgctgcg caacgtggct tgtcgcggcg gtgtcggggg ttcctggccg tcgtgcgcag
     1141 tcatgcatga gcttggtatt tgtcaaattc tttgcggaaa acgacgattt gcggcttgcc
     1201 ggggtggttt gatgcgtggt agtgacccac acgtccgaca tcggattacc tgacaaacca
     1261 tcaaggagta gaaatgcgtt cgttttcagc aatgttccta gttctggcga ctgcagcgac
     1321 gggctgcacg cactttcata ccaacatgct ttcggagtcg atcaaagcct tctctgcgaa
     1381 ggcgaagccg gccaaccaaa aagtgtgggt cgcttccgtc gcagacagca atgcctgtga
     1441 tagcggctgc cagctccccg gctcgtgtgg caagtcgtcg agcccgctcg gcggcaaggg
     1501 cctgtttggt ggcatgggcg gcggcggcag cgacaagctg gcctacgaag tcatcaccaa
     1561 ctacctgacc cagaaccaga cgatgaaggt cgtcgaaagc caccgccaca actacgcgac
     1621 ggacacgcgg gtcgaaacgc gcaagaagct cgatgtcgcg atggaaggca aagccgttgc
     1681 gtcgagcatg tcgtgtgaag acctgtgcct cctcgatgaa gcgaagaagc gcaacgccga
     1741 caaggtgctg acctatcaca tcatcgacat gggcgccaag aagctgacga ttcactatcg
     1801 cctgagcgat gtgaagagcg gcaccgtcga cgcttcgtac accctgcacg tcagccaccc
     1861 gcagcaagcg ggcgacgtca gcttcgtcga gccggcaccc tagtcagcgc gcttagtagg
     1921 gcagggggga cagcaaaact tcggcgcaca ggccgaagtg atcgctggga tagacgccat
     1981 ctgtatcggc gcgatcgagg acgatgcggc agtccgtaac ggtgccgcgt ccgtcccgac
     2041 tcgcgggact tacaaaaatg tagtcgagcc ggcgatcgtt ttgcaaaaag cgcagccgct
     2101 gcgtatacgg attccgtcgc gaccaggtgt agcccggtcc tttcgggtca tcggttgcgc
     2161 aaaggaatgc gtcttggtag tacgtgcgcc gtcctcccag cgtatgcagt ccgcgcagaa
     2221 aacgcagctc atcactatcg ggcgtcgcgt taaaatcgcc ggccaacagc gtcagcttgg
     2281 cgccccctga aacactctgc gcatgggctt ttaggaatcg gtctatcgct tcgacttgct
     2341 gttcgcgcgc gattccgtcc gtcatccggt agtgcagatg tgtcgtcgct gcgatcactg
     2401 tccccacttg cgtttggatg tccgcgatga gcagcacccg tcgttcttcc ggacgttcat
     2461 ttggcagctc atggacgacg tgggcggcaa tcggcagccg cgacagaatc gccagacctt
     2521 ccgttccgcc gccccacgct gaagccggcg caaacacaaa cgacattccc aagtgctctg
     2581 caagctgttc cgcggtgttg cgcagcgtac cgggaatttc gcgaacttct tgcagcgcca
     2641 ccacatccgc gtcgagcgca cgcagctcct tttggcacaa cggaaagcgt ttttcgaacg
     2701 gcccctgttc cccccacagg ttcagtgtga ccaaccggag tcggatgttt tccgtcgctg
     2761 ctgcgttttc gatggggcgt gtcatccgcg gtttcgcaca ggtccgtgcg gtccatagta
     2821 gatgcagcgc gagtccaccg tttcccacaa cgtcaccgag cagagaccag gcagcgacgg
     2881 gaaaatgcga tcgaagacat accgcgcgat gacttcgctc gtgggatttt caagccccgg
     2941 aatttcgttt aagagccgat gatcgatcga gtcaatcacc ggctgcacca cccggtcgag
     3001 atcataaaaa tcgatcagga aaccggtctc ttcattggct tcaccgccga gcaccacatc
     3061 aattttgaac gagtgcccgt gcatccgccg acagcgatgg ttgtcgggga ctttgggcag
     3121 aaaatgggct gactcaaagc ggtactcgcg aactagctcc acgtacatcg atcggtcctg
     3181 tgtctttcgc tggctagttt gcgccgtggc attttttgta ttttttgccg ctgccacatg
     3241 gacaggcatc gttgcgaccc actgcggtct gcggacgctg cttggattgg ttgcgcagct
     3301 gtctttgcga cgcgagcagc ggcgagacct gatcacgcat gaagcgatag cgattttcgt
     3361 acatcttcac aagctgcggc tgcgacgaaa gcaggttgga taccagcaca aacagactgt
     3421 tggcatcgac gcccggcgtc acttccagca ccgccggttc ctttttgggc agcgaaaagt
     3481 ttacgacgcc aatcttctgc gccgcaccgc ccggaccggg ggacaccacc gtccagacca
     3541 cgcgcttaca gtcgcacttg ggttcgatgc agtaggcatc ctgcagacct atcgaaacct
     3601 ggggcgcggg ctttggctct tcaccttcgg cggcgggctg cttttcatcc gatgtcatcg
     3661 tgaaaagcgg aatcccttcg gcgcggaaga cgtgctgcca cagcaccatt tcacctggct
     3721 ttacccccga ccagtcgaag tagcggaagt gcagcgtctt gccaaactca cgggtgtccg
     3781 tgtagtgctt gcgcaggttg tcgagctggc cgctctggat ggcgtcgcgc acgcccttgc
     3841 gcagaatctc ccgtccgggg gcattctggt tgtcgagaaa atcgacccgc tgcgtgccca
     3901 agtcgaccgt catcaccacc ccgccgaccc gcaagtgagc ggtcgcgcag tcacaggcat
     3961 gatcaccgca ataaaattcc tcgactggct ggccagcgac ggaaaagggc ggatcttttt
     4021 tcaggttttc ggtttccgga acgtctcgat agggaatcag ggccatggct ggttttgtct
     4081 ctgcaaacgt gcgtaaaaag ggaagttagg gtggcagcgc taggcttcac caccggccag
     4141 gattcgttcc gcaaccgccg cgcggtttgt ggtcagacag gctgcggcac aggcttcctc
     4201 gtcgtcggac ggagcgcgac cttcggcttt ggccagctcg cgtaggtaga cggcgcgcag
     4261 ctcgtcatag ccgaccgaca gatcgcggtt taggaccagg tagtggtaca tcgaaaaatg
     4321 cgaaagctcg tcgcgggctt tgcgcagacg ggtcgcgatg acttcttcgc tgtcggtgcc
     4381 gcggcctcga aggcgcgctt ccaagatcgg cagcgacggc ggcaggatga aaatcagacg
     4441 tgctgcgtcg gcaaactcgg ccaggatgcg ggtcgcgcct tggtaatcga tgtcgagcag
     4501 gactttgcag ccgcttgcca gcgcttgccg cacggtttca atcccggtgc cgtagcgtcg
     4561 tccgtgaacc cagttccatt cggcaaacgc gccgcggctt accatgtcgt cgaacgtcgc
     4621 gtcgctgacg aagtgataat cgactccgtc acgctcgcca ctgcgcggcg gacgggttgt
     4681 atacgacacc gaaaaccgca gctcgggcct ttccgcgagc agtcgtttgc acagcgtggt
     4741 ttttccagcc cccgacgggg atgacaccac aagcaaaaga cctgtgccgg cttgcgtcat
     4801 cgcttaggcc tcgtcacgag cggacgcttc gccatcggca ccgagcgcgc tcgacgacgc
     4861 accaccaatg ccaccgcgtt ccagtcgttc cgccaccgtt tcggtttgca gcgccgacag
     4921 cacgatgtga tccgaatccg taatcaggat cgatcgcgtc ctgcgtccct gcgtcccgtc
     4981 gatgagtcgt ccggccttgc gcgcttcttc tttgacacgc ttcatcggcg ccgattctgg
     5041 actgacgatc gccaccacgc gctcagcgac cactgcgttg ccgtatccga tgttgactag
     5101 acgcacggcc acaccacaaa acaaaagacc atctgcgcga agtttgtcga ggctgggcta
     5161 ttccacattt tgaacctgct cgcggagccg ctctagctcc gctttcatcg cgacgacgcg
     5221 cgccgccact tccgccgact gggctttcga cgtcagggtg ttggc
//
LOCUS       DAORGN010000278         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8020_length_5227_cov_6.333913, whole genome shotgun sequence.
ACCESSION   DAORGN010000278 DAORGN010000000
VERSION     DAORGN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_5227_cov_6.333913"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKL17_20125"
     CDS             <1..307
                     /locus_tag="PKL17_20125"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNK47102.1"
                     /translation="VGWASTPLVAQGKGGRGAPHANVLPTNLIMMKSLTVMGCPTVIS
                     TIHDPTIRSLRLQTIFDWAKSGAIRPHISHRFALAEFRQAMKAKWNGEVTGSCVIHP"
     gene            337..1599
                     /locus_tag="PKL17_20130"
     CDS             337..1599
                     /locus_tag="PKL17_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNK47103.1"
                     /translation="MTDRLSVKPQRLWQSILALGSIGSYLDEGSGLRGVNRLALTSAD
                     GEGRRHVVEQMKALGLSVSIDRIGNVLARRSGQDDSLAPVMMGSHIDSVPTAGMFDGC
                     LGVLGALEIIATLNDAGRQTLRPITVAFFTDEEGSRFGTDMLGSAVATGRIPLAEAYA
                     LVDRNGLSVKGELQKIGFLGEADERLAAPYAYLECHIEQGPVLRSANVDIGVVTGVQS
                     ISWHEVTIVGRSAHAGTTPMSLRADASVAASRLNLRLREMAQSGNYGPDMRATMGAMN
                     PVPGLVNIVPGKMTVTVDLRNPDDANMRAAEADIIAFYEKVAREEGVQIRWRQTARTD
                     CVRFDAGIQKRVSAAAAQRGFAQQAIVSGAGHDAQELAHLTRAAMVFVPGEFAGISHN
                     PREFSTEQQCANGVNVMLDVVLSLADEK"
     gene            1656..1979
                     /locus_tag="PKL17_20135"
     CDS             1656..1979
                     /locus_tag="PKL17_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47104.1"
                     /translation="MNLGRVIGTVVATQKYEGLEGKKFLVVQPLDHALRTAGDPIVAV
                     DTVRAGPGELVYFVNSREAALACDPWFVPVDAAIVGIVDDVACEPATAAEGVFVSDAA
                     TGGAS"
     gene            1976..2248
                     /locus_tag="PKL17_20140"
     CDS             1976..2248
                     /locus_tag="PKL17_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47105.1"
                     /translation="MKLCRVLGSVVATAKHPTYVGRKLLSVQPLDEKGAAIGSSFLAV
                     DDVQAGPGDVVLVMQEGNGVRQLLKAKDLPIRSLIVGIVDRVAVSS"
     gene            2245..3006
                     /locus_tag="PKL17_20145"
     CDS             2245..3006
                     /locus_tag="PKL17_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNK47106.1"
                     /translation="MNAAESKLRAELLDYCKRLHARGWVANHDGNLSVRLRPGQLLCT
                     PTATSKADVRDETLLTVDESGNRLTGKGKPFSELSLHLAVYKARPDVQAVIHAHCPYA
                     TALAASGRALDQPFLPEAVVSLGPTIPLVPLTAPGNPQVAALSPLVPFHDVVLIAGNG
                     VLGYGDSLEQAYLRVELLEHLCRIFLLALPVGGVKPLPDTMLASLLEARKKAGLGPQA
                     RGLVVPALSPLPAASSSDRLLSVIREEVATMLSKS"
     gene            3178..4278
                     /locus_tag="PKL17_20150"
     CDS             3178..4278
                     /locus_tag="PKL17_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47107.1"
                     /translation="MAFGMLLLSSCSGGATAGGGTDVPPDALCQPGQKTCVGNVLNTC
                     TDIGTYAPTSECASSLVCDPQLGCVACAPGTNTCVQNEVHSCNSDGNPGGLVMACDFA
                     QTCRGGSCVDSCELAASEFIYLLDSSNQLLSFEPRMDTAAGAVKVVGKVVCPTATAPN
                     SMSVDRRARAWLNYDDGKLYKIPTNNPASCADSGYQTGVVPTAKVGMGFVSDSPGAKV
                     ESLYLASFGTASQLWKLNPLDPTPIVSTKIGNFASSPTSPEMTGTGAAELYAYFPAAD
                     ATHRIIRVNKTTAQADLTWSLPPLASSPGAWAFAQWGGRYYVFVTESNISRVYRWDPN
                     AAAGSQWVRVQDNLPTRIVGAGVSTCAPTTVG"
     gene            complement(4290..>5227)
                     /locus_tag="PKL17_20155"
     CDS             complement(4290..>5227)
                     /locus_tag="PKL17_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNK47108.1"
                     /translation="PKTIVDSQSYMDPKNSALHHSVNRIGEVCEQHMKVFHIETVLNN
                     RMFSGPLDFLMKNEDEFTEADTLKFQAIKWSLKKTPRALRREIFMRVPAAYELIAVHA
                     GKADPVHQKTLAKSWEQYSIPVDGQADILITGIPYISPYNVNSKALNPLLVQVMALGY
                     FFNMYRNKCPLKKGGTLIFTHPCTDAFDPVHHPSYIEFFNRLLPESRDAYYLEKRYQD
                     EFAYNPTYIEMYRRGNAYHGAHPFYMYYWGQAGREWCGEVISVGADNRTVPDLFGWKR
                     ADTLAEAISMGRARHGRSSQITMLHHPPILIADVK"
BASE COUNT          968 a         1494 c         1669 g         1096 t
ORIGIN      
        1 tgtgggctgg gcctccactc ctttggtcgc gcaagggaag ggcggtaggg gggcccccca
       61 tgcgaacgta ctgccgacca acctgatcat gatgaagtct ctgactgtga tgggttgtcc
      121 gacggtgatt tcgacgattc acgatccgac gattcgttct ttgcggctgc agacgatctt
      181 cgactgggca aagtcgggcg cgatccgtcc gcacatctcg catcgctttg cgcttgcaga
      241 gtttcggcaa gcgatgaaag caaagtggaa cggagaagtc accggaagct gtgtgataca
      301 tccgtagaac cgttgtcgat aggaaggaca gaagaaatga cagacagact ttcggtgaag
      361 ccgcagcgcc tttggcaatc cattttggca ctcggatcga taggaagtta tctggatgaa
      421 ggatcgggac tgcgtggcgt aaaccggctg gcgctcacat ctgccgacgg agaaggtcgt
      481 cgtcatgtgg tcgaacaaat gaaagcgctg ggtctgtctg tttcgatcga tcgcatcggc
      541 aatgtgcttg cgcggcgatc cggacaggat gactcgctgg ccccggtgat gatgggttcg
      601 cacatcgaca gcgtgcccac cgcgggaatg ttcgatggct gtttgggagt cctgggtgcg
      661 ctcgagatca tcgcgacgct caatgacgcg ggccggcaga ctctgcgtcc gatcacggtt
      721 gcgtttttca ccgatgagga aggatcgcgc tttggaacag acatgctcgg aagcgctgta
      781 gcgacgggac ggattccgct tgcagaggcc tacgcactgg tcgatcggaa cggactttcg
      841 gtgaagggag agctacagaa gatcggtttc ttgggtgaag cagacgaaag acttgcggct
      901 ccctatgcct atctggaatg tcacatcgag caaggtccgg tgctgcgcag cgcgaatgtt
      961 gacatcggtg tggtgacggg cgtgcagtcg atttcgtggc atgaagtgac cattgtcgga
     1021 cgcagcgcgc atgccggaac cacgccgatg tctctgcgcg ccgatgcgtc ggtggctgcg
     1081 agtcgactga acctgcggct gcgcgagatg gcgcaaagtg ggaactacgg tccggacatg
     1141 cgggcgacga tgggtgcaat gaatccggtg ccggggctgg tcaacatcgt gccggggaaa
     1201 atgacggtga ctgtggatct gcgcaatccc gatgacgcaa acatgcgcgc agccgaagcc
     1261 gacatcatcg cgttttacga aaaggtggcg cgcgaggaag gggtgcaaat tcgctggcgc
     1321 caaacggcgc ggaccgactg tgttcgtttc gatgcgggga tccaaaagcg agtttctgcg
     1381 gcggcagcgc agcgtggttt tgcgcagcaa gcgattgtgt cgggggccgg gcacgatgcg
     1441 caggaactgg cgcatttgac gcgcgccgcg atggtgtttg tgccgggtga gtttgccggc
     1501 atcagtcaca acccgcgcga gttttcgacg gagcagcagt gtgcaaacgg cgtcaacgtg
     1561 atgctcgatg tcgtgctgtc gctggccgat gaaaagtagg tcgcttggtt gggcttgacg
     1621 cgcttaggct gggccgccta tgttgccgcc tgcacatgaa cctcggtcgc gtcatcggta
     1681 ctgtcgtcgc cacccagaaa tacgaagggt tggaaggcaa aaaattcctc gtcgtgcagc
     1741 cgctcgatca cgcactgcgg acggcaggtg atccgattgt cgcggtcgac accgtgcgcg
     1801 ctggaccggg cgagcttgtc tattttgtaa actcgcgcga ggcggcactg gcgtgcgatc
     1861 cgtggtttgt tcccgtcgac gcggcgattg tcgggattgt cgatgatgtc gcgtgcgaac
     1921 cggcgaccgc cgccgaaggc gtgttcgttt ccgacgccgc gacgggaggt gcatcgtgaa
     1981 gctctgccgc gtactcggtt cggtggtggc gacggccaaa catccaacct atgttgggcg
     2041 gaagctgctt tcggtgcagc cgctcgacga aaaaggtgcg gccatcggca gttcgttttt
     2101 ggcggtcgat gatgtccagg ccggtcccgg cgatgtggtg ctggtgatgc aggaaggcaa
     2161 cggcgtccgt cagctgctca aagccaaaga cttgccgatt cgctcgctga tcgtcgggat
     2221 tgtcgatcgg gtggcggtgt cgtcatgaac gcggcggagt ccaagctccg cgccgagctg
     2281 ctcgactact gcaaacggct gcatgcgcgc ggctgggtcg ccaaccacga cggcaacctg
     2341 agcgtgcggc tgcgtcccgg ccagcttctg tgtacaccga cggcaacctc gaaagcggac
     2401 gtccgtgatg agacgctttt gaccgtcgat gaatcgggca accgtctgac cggcaagggc
     2461 aagccgtttt ccgagctgtc gctgcacttg gcggtgtaca aagcgcggcc cgacgtccaa
     2521 gcggtgattc atgcgcactg tccgtatgcg acggcgctcg cggcatcagg tcgcgcgctt
     2581 gaccagccgt ttctgcccga agcggtggtg tcgcttggac cgacgattcc gcttgtgcca
     2641 ctgacggcac cggggaatcc gcaagtggcg gcgctgtcac cgcttgtgcc ttttcacgat
     2701 gtcgtgctga ttgctggaaa cggcgtgctg ggatatggcg acagcctcga acaggcgtac
     2761 ctccgcgtcg agctgctcga acacctgtgc cggatctttt tgctcgcgct gcccgtcggt
     2821 ggggtgaagc cgctgcccga cacgatgctg gcgtcgcttt tggaagcgcg caaaaaagct
     2881 gggcttggtc cacaggcccg cggtctggtt gttccagccc tgtcgccgtt gcctgccgca
     2941 tcgtcgtccg atcgtttgct gtccgtgatt cgcgaggaag tcgcgaccat gctgagcaaa
     3001 tcctaagccc atctttctgc gcgtccgttt gcgctgtttg ccgtcggaac catcgcttcg
     3061 cgggttgagc gattttgcag ttttcgcgaa actagaactg cggatctcat tcgatcggcg
     3121 aaccaggggg aaacgatgcg caagtcctta ttcactagtc ttctgtctag cttcacgatg
     3181 gcttttggca tgctgctttt gagcagctgc agcggcgggg ccactgccgg cggcggtacc
     3241 gacgttccgc ccgatgcgct gtgccagccc ggacaaaaaa cctgcgtcgg caatgttctg
     3301 aacacctgca ccgacatcgg cacgtatgca ccaacctccg agtgcgcaag ctcgctggtt
     3361 tgcgacccgc agcttggttg cgttgcgtgc gcgcccggca ccaatacctg cgtccaaaac
     3421 gaagtccatt cctgcaattc cgacggcaat cccggcggac ttgtgatggc gtgcgacttt
     3481 gcccaaacct gccgcggcgg ttcgtgcgtc gattcatgcg agctggcggc gagcgagttt
     3541 atttatctgc tcgattccag caaccagctg ctgtcgtttg aaccgcgcat ggacaccgca
     3601 gccggcgccg tcaaggtcgt cggcaaggtg gtgtgcccga cggccaccgc gccaaactcg
     3661 atgtcggtcg atcgccgcgc ccgcgcctgg ctaaactacg acgacggcaa gctgtacaag
     3721 atcccgacca acaacccggc gtcctgcgcc gacagcggct accaaacggg cgtcgttccc
     3781 acggccaagg tgggaatggg ttttgtttcc gacagccccg gcgccaaagt ggagtcgctg
     3841 tacctggcgt cgtttggcac tgcatcgcag ctttggaagc tcaacccgct cgacccgacg
     3901 ccgattgtgt cgaccaaaat tggcaatttt gccagcagcc ccacgtcgcc agaaatgacc
     3961 ggcaccggcg cggcagagct ttatgcgtac tttccggcag ccgatgccac ccaccgcatc
     4021 attcgcgtca acaaaaccac cgcacaggcc gatttgacct ggtcgttgcc gccgctcgca
     4081 tcgagtccgg gcgcgtgggc atttgcccag tggggcggtc gctactacgt gtttgtcacc
     4141 gagagcaaca taagccgcgt ctatcgctgg gatccaaacg ccgccgcggg ttcgcagtgg
     4201 gtgcgggtgc aggacaacct gccgacgcgc atcgtcggtg ccggtgtgtc gacctgcgcg
     4261 ccgaccaccg tcggttagaa ccccgaggct tacttgacgt cggcgatgag gatcggcggg
     4321 tggtgcagca tcgtgatttg cgagctgcgc ccgtgacgag cccgacccat cgagatcgct
     4381 tccgcgagcg tatcggcgcg cttccaaccg aataaatccg gcaccgtgcg gttgtcggct
     4441 ccgacggaaa tgacttcgcc acaccactcg cgaccagcct gaccccagta gtacatataa
     4501 aacgggtgcg cgccgtgata agcgtttcct cgacggtaca tctcgatgta cgtcgggttg
     4561 tacgcgaact cgtcttggta gcgtttttcc aggtaatagg cgtcgcgtga ctcgggcaac
     4621 agccggttga aaaactcgat gtagctcggg tgatggaccg gatcgaacgc atcggtgcac
     4681 ggatgcgtaa agatcagcgt gccgcctttt ttgagcgggc acttgttgcg gtacatgttg
     4741 aagaagtagc cgagcgccat cacctgcacg agcagcggat tgagcgcttt ggaatttacg
     4801 ttgtacggcg agatgtacgg aatgccggtg atcaggatgt cggcctgacc gtcgacggga
     4861 atcgagtact gttcccacga cttcgcgagc gttttttgat gcaccggatc ggctttgcca
     4921 gcgtgaaccg caatcagttc ataagccgcc ggcacccgca taaagatttc ccgtcgcagc
     4981 gcccgcggcg tttttttcag cgaccacttg atcgcctgaa acttcagcgt gtccgcttcg
     5041 gtgaactcgt cttcgttttt catcaggaag tcgagcggtc ccgaaaacat ccggttgttg
     5101 agcaccgttt cgatgtggaa caccttcatg tgttgttcgc acacttcacc gatgcgattc
     5161 accgagtgat ggagcgccga gtttttcggg tccatgtacg actgcgagtc gacaatcgtc
     5221 ttcggcg
//
LOCUS       DAORGN010000279         5208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8061_length_5208_cov_5.031438, whole genome shotgun sequence.
ACCESSION   DAORGN010000279 DAORGN010000000
VERSION     DAORGN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_5208_cov_5.031438"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..912)
                     /locus_tag="PKL17_20160"
     CDS             complement(85..912)
                     /locus_tag="PKL17_20160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK47109.1"
                     /translation="MSSPQPEPTDAEVPSHAPPAPSPLPRDPFMSQPLIVRFAEVGKS
                     FRRFDYQPFLLRNLVLRLTGRSHKPREFWPIRHVSFDIRRGETVGVIGQNGSGKSTLL
                     RLIAGACYPSEGTISVRGRIAPLLALGAGFHPDMTGRECVEVNGTALGLTRAEIRARM
                     DNILHFAELTDFLDTPVRYYSSGMLARLGFSVAVHTDPDLLLVDEVLSVGDHAFQHKC
                     LQRIQDIRASGTTILFVSHDADTVERLCDRVLWLREGRLLRDGDPKTVLAEYLATDG"
     gene            complement(916..2304)
                     /locus_tag="PKL17_20165"
     CDS             complement(916..2304)
                     /locus_tag="PKL17_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47110.1"
                     /translation="MLTTPRRLPWLGLVAIALLWPVAQHFRIDQVRCESWPAPLVRLG
                     LCLLYLLAVGLLFFVWQRLLQKSESGVFSFRHALVTAVGWHGAALFCPPFLSDDPLFY
                     LAIGRVLHAQHPSLHAPLVDVLGPGDAVVRLLPLHWQAGTSAYQAGFHALAWLVESLP
                     GLSIGGRLTVYQSLSALVALVTTLVCARLAVRCGKPAAYGATLFGLSPLVLLEGTLSA
                     HNDVWLACLLALSALAWLSSRRVLCRLGLLLGLLVKLSAMLPLLTMTLSHAVPFVRRH
                     KRWIALCLVLGLTVLAFLWRFFPPFPQLKGHLTSVLFGSPDLPWDHCTRSIECLPRSI
                     LRYGLDRPDWSYRVGVAFRLLGGLWLVRVACADKNPLSATAWGLLSYYLFWHGWAQTW
                     YFLPVLPLRLFLSKPRGRALEVLCGSGCGYYALALYRNCTTEPWQVAVCELLEGLATV
                     LPPALCLCRRND"
     gene            complement(2308..3285)
                     /gene="fmt"
                     /locus_tag="PKL17_20170"
     CDS             complement(2308..3285)
                     /gene="fmt"
                     /locus_tag="PKL17_20170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNK47111.1"
                     /translation="MRTVFMGSPEFAVPCLHSLHQHSQLVAVVCQPDKPAGRGQKLQP
                     PAVKVAAEALGLPVLQPTALRPSKSNFVQELQAYSPDLVVVVAYGRILPPEVLALPKF
                     GCWNVHGSILPRYRGAAPIQWSLIRGETETGVTLMQMDEGLDTGPMLRTLTIPIEPGD
                     ISGTLHEKLSRLGAQLLDQAIPDLAAGKSPVPIPQDHALSTLSPMLDKEHGRADFTRS
                     ARLVCGQLRGVDPWPGGYTLLPSGIAGPEPTLLKLFLPRVSSGSGRPGEILGVDRDGL
                     HIACGDGAVVIAEAQLPGRKRLPMQALCAGLKLPRGTILDDGRPNPLLG"
     gene            complement(3337..3951)
                     /locus_tag="PKL17_20175"
     CDS             complement(3337..3951)
                     /locus_tag="PKL17_20175"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNK47112.1"
                     /translation="MGSAGILGDRRGRHDAFFKKAQRENFAARAVYKLQEIDERYRLF
                     TPGKRVLDLGCRPGSWLQYAGQKVGSQGALVGIDRQPLQVAVKGARILIGDVFAISPD
                     ELRGELPAFDVVLSDMAPDTTGIRHVDQARSEGLFERALDLADQLLFPGGHFLGKLFQ
                     GPDFQRLRARLQSSYSEVRIVKPDSSRKESIELYLLALRRKAPL"
     gene            complement(3970..4716)
                     /gene="rplC"
                     /locus_tag="PKL17_20180"
     CDS             complement(3970..4716)
                     /gene="rplC"
                     /locus_tag="PKL17_20180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNK47113.1"
                     /translation="MPAARTGLLGKKIGMTQMFTPQGDRVGVTVVHVGNCVVLGKRTI
                     EKDGYSAVQIGYGEKPLRLCNKAELGLFSKLNVKPVRFIQEFRMDPKQADTFEVGKTL
                     APSQVFKALTFVDVVGQSKGKGFQGVIKRHHMAASVDSHGSHEFFRHGGSIGCRLTPG
                     RVHKGKRMTGHMGDDRVTVESIALMDVRDAEGLLVLRGSVPGGNNGYVTIRNSTKRPN
                     AIVRLTEAEQDQKKRAAGVAKKAAGGAKKK"
BASE COUNT         1069 a         1618 c         1647 g          874 t
ORIGIN      
        1 gatggcgtga cgatggctgg ggatggcgtg acgagggctc gggatggcgt gacgagggcg
       61 gggcgttttc ggcgaggtgg ggggttagcc gtcggtggcg aggtattcgg cgagcacggt
      121 cttgggatcg ccgtcgcgca aaagccggcc ttcgcgcagc cacagcacgc gatcgcacag
      181 tctttcgacg gtatcggcat cgtgtgaaac aaacaggatc gttgtgcccg acgcgcggat
      241 gtcttgaatc cgctgcagac acttgtgctg aaacgcatgg tcgccgaccg acagcacttc
      301 gtcgacgagc agcagatccg gatcggtatg cacagcgacc gaaaaaccca gccgcgccag
      361 catccccgac gagtagtagc gcaccggggt atccaaaaaa tcggtcagct cggcaaagtg
      421 caggatgttg tccatgcggg cgcgaatttc ggcgcgcgtc agcccgagtg ccgtgccgtt
      481 gacctcgacg cactcgcggc ccgtcatgtc gggatggaag cccgccccga gcgccaacag
      541 gggcgcaatc cgaccgcgca ccgagatcgt gccctcgctg ggataacagg ccccggcaat
      601 caggcgcaaa agcgtgctct tgcccgatcc gttttgaccg atgacaccga cggtttcgcc
      661 gcgccggatg tcaaagctga cgtgacgaat cggccagaac tcgcgcggct tgtgggaccg
      721 tccggtcagc cgcaaaacca ggttgcgcag cagaaacggc tggtagtcga accggcggaa
      781 gctcttgccg acctcggcga agcgcacgat cagcggctgg gacataaacg gatcgcgggg
      841 aagcgggctc ggtgccggcg gggcgtggct tgggacttcc gcgtcggttg gttcaggttg
      901 cgggctggac ataagctagt cgttccttcg gcaaaggcac agggctgggg gcaggaccgt
      961 cgcgagtcct tcgagtagtt cacagacggc gacctgccac ggctcggttg tgcagttgcg
     1021 atacagcgcg agcgcataat acccgcagcc agagccgcac agcacttcta gcgcacgtcc
     1081 gcgcggcttg gaaagaaaca gccgcagcgg caagaccggc agaaagtacc aagtctgcgc
     1141 ccagccgtgc caaaacaggt agtagctgag cagtccccac gccgtcgctg aaagcgggtt
     1201 tttgtcggca cacgcgacgc gcacaagcca cagcccgccg agcagtcgaa acgccacccc
     1261 gacgcgatac gaccagtcgg gacgatccag tccatagcgc aagattgatc gcggcagaca
     1321 ctcgatagag cgcgtgcagt ggtcccaagg taggtcgggg ctgccgaaca gcaccgacgt
     1381 cagatgtccc ttgagctgcg ggaagggggg aaaaaaacgc cacaaaaacg ccagcaccgt
     1441 cagacccaga accagacaga gcgcaatcca gcgcttgtgg cgtcgcacaa acggaacggc
     1501 gtgtgacagg gtcatcgtca gaagcggcaa catcgccgac agcttcacca gcaggccgag
     1561 caaaagccca agtcggcaaa gcacgcgacg cgacgacagc caggccagcg ccgacagcgc
     1621 cagcaaacac gccagccaga catcgttgtg cgccgacagc gtgccttcca aaagcacaag
     1681 cggtgacagc ccaaacagcg tcgctccgta agccgccggc ttgccgcagc ggaccgccag
     1741 gcgcgcacac acaagcgtcg tcacaagcgc caccagcgcc gacagacttt ggtacaccgt
     1801 cagccgaccg ccgatcgaaa gacccggaag cgactcgacc agccaggcca gcgcgtggaa
     1861 accggcttga taggcgctgg tgccggcttg ccagtgcaaa ggcagaagtc gcacgaccgc
     1921 atcgcctgga cccagcacgt caacgagcgg cgcatgtagc gacggatgtt gcgcatgcag
     1981 cacccgtcca atcgccagat aaaacagcgg atcgtccgat agaaacggcg ggcaaaacag
     2041 cgcggctccg tgccagccaa ccgcggtgac aagcgcatgc cggaacgaaa aaacgcccga
     2101 ttcgctcttc tgtagaagcc gctgccaaac gaaaaacaga agccccaccg cgagcagata
     2161 caaaagacac agcccaagcc gcacaagtgg ggcgggccag gattcacagc gcacctggtc
     2221 gatgcgaaag tgctgcgcca ccggccacaa aagcgcgatg gccacaagtc ccagccaggg
     2281 caaacggcgc ggcgtcgtca gcaagtctta gccaagcagt ggattgggcc gtccgtcgtc
     2341 aagtatcgtc ccgcgcggta gcttaagccc cgcacacagg gcttgcatcg gcaggcgctt
     2401 gcgaccgggg agctgcgctt cggcaatcac cacggcaccg tcgccacagg caatgtgcag
     2461 accgtcgcga tcgacgccga ggatttcgcc tggacgccca cttcccgacg aaacgcgcgg
     2521 caaaaacagc ttgagcagcg tgggttctgg cccggcaatt cccgacggca aaagcgtata
     2581 tccacccggc cacggatcaa cgccgcgaag ctggccgcag accaggcggg ccgatcgggt
     2641 aaagtcggcc cggccatgct ctttgtcgag catcggcgac agcgtcgaga gcgcatggtc
     2701 ctggggaatc ggtacgggcg attttccggc ggccaagtcg ggaatcgctt ggtccagaag
     2761 ctgcgcgccc agccggctta acttttcatg cagcgtgccg ctgatgtcgc cgggttcgat
     2821 gggaattgtc agcgtgcgca gcatcggccc ggtgtcgagc ccttcgtcca tctgcatcag
     2881 cgtcacgccg gtttctgttt cgccccgaat gagcgaccac tgaatcggcg cggcacctcg
     2941 gtaacgcggc aggatcgatc cgtgcacatt ccagcagcca aacttgggca gcgccagcac
     3001 ttccggcggc aaaatccgtc cgtaggcaac gacgacaacc aagtcgggac tgtacgcttg
     3061 cagctcctgc acgaagttcg acttgcttgg acgcagcgcc gtcggctgta acaccggcag
     3121 ccccagcgct tcggcagcga ctttcaccgc gggtggctga agtttttggc cgcgtccagc
     3181 cggcttgtcg ggctgacaga cgacggcaac gagctggctg tgctgatgca gggaatgaag
     3241 gcacggcacc gcaaactcgg gcgagcccat aaacacggta cgcatgattc gtaggaacct
     3301 cccagtcggg agacaaaggg gaaggaaacg cggcggctac agcggagctt tgcgtcgcag
     3361 cgccagaaga taaagctcga tcgattcctt gcgcgagctg tccggcttga cgatccgcac
     3421 ttccgaatag ctcgactgga gccgcgcccg caggcgctgg aagtccggac cctggaacag
     3481 cttgcccagg aagtgaccgc cgggaaaaag cagctgatcg gccagatcga gcgcgcgttc
     3541 aaacaggcct tcgcttctgg cttggtcgac gtgacgaatc cctgttgtgt cgggagccat
     3601 gtcggacagc accacatcga aggccggcag ctcgccgcgc agctcgtcgg gagatatcgc
     3661 gaagacatcc ccgatgagaa tgcgcgcgcc cttgacggcg acctgcagcg gctgccgatc
     3721 gattccgacc agcgcgcctt ggcttcccac cttctggccc gcgtactgaa gccacgaacc
     3781 aggacggcag ccgaggtcaa gcacacgctt gcccggcgta aacaacctgt agcgctcgtc
     3841 gatttcttgc agcttgtaga ccgctcgcgc cgcgaagttt tcgcgctgcg cttttttgaa
     3901 gaaggcgtcg tgacgccctc ttcggtctcc gagtatgccg gctgatccca tcgacgagcg
     3961 ccagggtgat tacttcttct tggctccgcc cgccgccttc ttggcgacgc cagcggcgcg
     4021 cttcttctga tcctgctcgg cttccgtcag acgcacgatg gcgttcggac gcttggtcga
     4081 gttgcggatc gtcacatagc cgttgttgcc accggggacc gaaccacgca gcaccagcag
     4141 gccttcggcg tcacgcacat ccatgagcgc gatgctttcg acggtgacgc gatcgtcgcc
     4201 catgtgaccg gtcatgcgct tgcccttgtg gacgcggcct ggggtcagac ggcagccgat
     4261 cgaaccaccg tgacggaaga actcgtggct accgtggctg tcgaccgaag cggccatgtg
     4321 gtgccgcttg atgacgcctt ggaagccctt gcctttgctc tggccgacga catcgacaaa
     4381 cgtcaacgct ttgaacacct gcgacggcgc gagcgtcttg ccaacttcga acgtgtcggc
     4441 ctgcttcgga tccatccgga attcctggat gaagcgaacc ggcttgacgt tcagcttcga
     4501 aaacaggccc agctcggcct tgttgcacag acgcagcggc ttttcgccgt agccaatctg
     4561 aaccgccgag tagccgtctt tttcgatggt gcgcttgccg aggacgacac agttaccaac
     4621 gtgcaccacc gtgacgccaa cgcggtcgcc ttgcggggtg aacatctggg tcataccgat
     4681 ctttttgccg aggaggccag tccgagctgc gggcattttg cttctctttt tctgcgtgtg
     4741 agggtttggt cgaaacaaga acccagcaca cctgacgcag tttcatggtg gaggctgggc
     4801 ggccccagtg cctgggacca tttgagaagc caaatagcta ccgcgccttg ggtttttctg
     4861 tcaagaacgc agcgcagctt gttgtttgcg gggtgtgaaa cacacgatgt ttcatgatga
     4921 aacatcacgc ggactgaaac ggtcgcgccg tggaatgttg ccgtcgcaga cttgcggact
     4981 gcatcggctg ggttcgcccc acgtggggtt ggcactgcgg ttgctctgtg ccagcgggta
     5041 aggagacgga ctgatgttgg aactggcaat tgccctttcg cttggcatcg gcgtgctgct
     5101 cgggatgctc ggcggtggcg gatcggttct gcttgtgcca gtgcttttgt cggtgctgaa
     5161 gctgccgacg aagacggcgc ttgcgacatc gcagatggtg atggcggc
//
LOCUS       DAORGN010000280         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8139_length_5179_cov_3.578454, whole genome shotgun sequence.
ACCESSION   DAORGN010000280 DAORGN010000000
VERSION     DAORGN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_5179_cov_3.578454"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1090
                     /locus_tag="PKL17_20185"
     CDS             239..1090
                     /locus_tag="PKL17_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47114.1"
                     /translation="MHLKTRIRRAAIAIGVCVLLFPREGYVTSHGVSAGFRYKGYSVA
                     KSIPSDPNATKADRAAIDAEMKESLLRGNPIAARAFAHYVQGKHPYVAVRSHLLPDVK
                     DETEGVFWRTSPVELLAGVEVTLFVKPNIPDVIRNQIPRDFLLSQLSPGGVLSKQGGL
                     SEFPKPTDSRPPEVVLEHGLYNVHQVVYAIHNRDRIRQSISTVVGPGSQRGIVLQAIA
                     AVLDGKPIPAALTDAALLLPPTPKTDTFALLLDLSPRTDAAQVQRWVSQLGDLQRSRY
                     VDTPQAK"
     gene            1614..1913
                     /locus_tag="PKL17_20190"
     CDS             1614..1913
                     /locus_tag="PKL17_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47115.1"
                     /translation="MSHDDHVPGDLLCDCSLCRHGRVRARFQAEPQIYEDYAKLAAWW
                     CLEKEFPNGKINEGSVFQLVESPLTKPNAALEHLRNTLRSLLGPTYGRQRGDHGA"
     gene            1903..2418
                     /locus_tag="PKL17_20195"
     CDS             1903..2418
                     /locus_tag="PKL17_20195"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNK47116.1"
                     /translation="MAPDELRAFGLLIKTRRKTARLSLERLAVLCGLSSSSIKHVERG
                     RVSLATCSALLATPELRLTVDDLPSFAREPHRQHRGDTALLRLSLSLTEFAAREDVLA
                     GVMRFAAHRELPELGEWAEAKLLTVHRLRSALYSEDELLPAWRACRSNRRDCAGFGAE
                     SDCLQSIAWGR"
     gene            complement(2419..3054)
                     /locus_tag="PKL17_20200"
                     /pseudo
     CDS             complement(2419..3054)
                     /locus_tag="PKL17_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(3024..4478)
                     /locus_tag="PKL17_20205"
     CDS             complement(3024..4478)
                     /locus_tag="PKL17_20205"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNK47117.1"
                     /translation="MWPNKVEAVTRIEKSLFDDRPRSLIQMATGSGKTLLAVTTLYRL
                     IKFGGARRVLFLVDRANLGEQAEKEFQSFRTPDDQRKFTELYTVQRLTSNTLGSSSKV
                     VISTIQRLYSVLKGEPELDPEAEAHTSFDSDDVTVKEPLPVVYNQAIPPEFFDIIFID
                     ECHRSIYSLWRQVLEYFDAHLLGLTATPAKHTYGFFHQNVVMEYPHERAVAEGANVNF
                     DVYKIRTQITAQGSTVEASPGVMLGYRDRLTRKTRWEAPDEDVSYEAKDLDRNVVAID
                     QIRLIIRTLRDQVLKDTFPERTHVPKTLIFAKDDSHAEDIVRIVREEFGQGNDFATKI
                     TYKVTGTKPADLIQSFRTSYNPRIAVTVDLIATGTDIKPVEIVMFLRTVKSRVLFEQM
                     KGRGVRVVSPDELRSVTPDAKAKTHFLIVDCVGVSEAKLADTRPLEQNPSVPLKALLE
                     HVASGGTNDDYLSSLAGRIARIDKATSSSTSCST"
BASE COUNT          924 a         1570 c         1610 g         1075 t
ORIGIN      
        1 gccggaccaa tgctcagtcg ggaaacaggg caggtgtttt ccccctcgtc gaggtgcaat
       61 tcccaccggg cgaatggact cgcagccggg acatgaatga aactggctgc tggtcgcccc
      121 tgctcacctc gggattcggc gggctgggac cgcgttggcg tcggttcgag agagaatgca
      181 tcggcaacta cgtgcgtggg cggcatggct ttggcccagc ttgcgctatc ctgaagtgat
      241 gcatctgaag actcggatcc gtcgcgcagc catcgcgatc ggcgtgtgtg ttctgctgtt
      301 cccgcgggag ggctatgtga ccagccacgg tgtctcggct ggttttcgat acaagggcta
      361 cagtgttgcg aagtcgattc cgtctgatcc gaatgcgacg aaggcagacc gcgcggcgat
      421 cgatgcagag atgaaggaat cgcttctgcg cggcaatccc atcgcggcgc gggcgtttgc
      481 gcactacgta caggggaaac atccgtatgt cgcagtgcgc agccacttgc ttcctgatgt
      541 gaaggacgaa acagagggcg tgttctggcg tacaagtccc gtcgagcttt tggctggggt
      601 ggaggtcacg ctgtttgtaa agccgaacat tccggatgtg attcggaacc agattccgcg
      661 cgacttcctg ttgtctcaac tatcccctgg cggagttcta tcgaagcaag gcggactgtc
      721 ggaatttccg aagcccaccg actcacgccc accggaggtc gtgctggaac acggcctgta
      781 caacgtccac caagtcgtct acgcgatcca caatcgggac cgcatcaggc agtcgatttc
      841 caccgtggtg gggccagggt cgcagagggg cattgtgctg caagccatcg cggcagttct
      901 cgacggaaaa cccattcctg ctgcgctgac ggacgccgcg ctgcttttgc caccgactcc
      961 caaaacggac acctttgcgc tgctgctcga tttgtcaccg cggacggatg ccgcacaggt
     1021 gcagcgctgg gtgagtcagc ttggcgacct ccagcgttcc cgttacgtcg ataccccgca
     1081 ggcgaaatag cgaggcgcgg ttcatcggtc tgggacgtgc gggatgcgtc ctcggctcgt
     1141 gcgactactt cctcgaaacc gtgtggccgt cttcgccaaa gatcattccc aactcctcca
     1201 atgtctgacg aagccgcgcg accgttgagt cgaaggcggg ccgcttgccc tgctcatagg
     1261 cattcagcgt cgatacggaa atgcctgcac agcgcgcaag ctcgcgctgg ccgatctgta
     1321 gggaggcgcg ggcttggcgg acctggactg gcgtcggaac cggacttggg agttcgattt
     1381 ttcttgcttg atctggtgaa ggcatgctag ccaagacgca aacagtgttc tcgttttaga
     1441 aacatagctc gaaatgctga cgccgcgcgg caacgcggcg ccagcggtgt tcgtcgggac
     1501 gtgaccccaa cagaacgagg cgcagcgtat ccgctggggg cctatggatc aagtctgcgc
     1561 gcggagggcc gtgctttgtc tgtttttaca cgctgttggt cgaggagtgc gccatgagcc
     1621 acgacgatca cgttcccggc gacctgctct gcgactgttc gctgtgccgt cacggtcggg
     1681 tgcgcgcccg ctttcaggcc gaaccccaga tctacgagga ctacgccaag ctcgctgcgt
     1741 ggtggtgcct agaaaaagag tttcccaacg gcaaaatcaa cgaaggttcg gtctttcagc
     1801 tcgtcgagtc gccactcacc aagcccaatg cagcgctgga acatctgcga aacacgctgc
     1861 gcagcctgct cgggccgacc tacggacgcc agcgaggcga ccatggcgcc tgacgagcta
     1921 cgcgccttcg gactgctcat caaaaccaga cgcaaaaccg cccgtctgtc gctcgaacga
     1981 ctggcggtgc tgtgcgggct gtcgtcatcg tcgatcaagc atgtagaaag ggggcgagtc
     2041 tcactggcga cgtgctcggc gctgctggcg acgcccgagc ttcgtctgac cgtcgatgac
     2101 ttaccgtcat tcgcccgcga gccgcaccgg cagcaccgag gagacactgc gctgctccgt
     2161 ctgagcttgt cgctgaccga gttcgccgcc cgagaggacg tgctcgccgg ggtgatgcgc
     2221 ttcgcggcgc accgggagtt acctgagctt ggcgagtggg ccgaggcgaa gctgctgacc
     2281 gtgcatcggc tgcggtcggc gctgtattcg gaagatgagc ttttgcctgc gtggcgggcg
     2341 tgtcggtcga atcgacggga ctgcgcaggt tttggggctg aatccgactg cctacaatcc
     2401 atagcgtggg gtaggtgact agctgcgagc ctcgcgtttt gcgatgacgg cggcgcgctg
     2461 ctggaggtcg gacaggacgc tttcgatctg ctccagcgcg gatcgcaggt cgtcggcgat
     2521 ttcttcggcc aggacgtgcg gctccggcag cttggccgag tcttcgaggc tttcgtcttt
     2581 gagccaaaag atgtcgaggc tgcacttgtc gcgcgccgcc agctcgtcgt aggaaaacac
     2641 ccgccagcgg ccttcgggat tctgtttttc gtcccacgtc gcctgacgct ggctgcgatg
     2701 ttcgggctta tagcaggcga caaactcatc caggtccgtg cgcgccatcc gtcgcgtctt
     2761 cagcgtgaag tctttgttcg tgcgcaggtc gtagaaccag acttttttcg tccacggctc
     2821 tttcgcaccc ggcttgcggt cgaaaaagat cacgttcgcc ttgacgcctt gcgcgtaaaa
     2881 gatgcccgtc ggcagacgga ggatggtgtg aacgtcgcac tcttgcagca gcttgcgccg
     2941 gattttttcg ccagcaccac cctcgaacag cacgttgtcg ggcaccacca ccgcggctcg
     3001 gccatgcatc ttgagcatcg accttatgtg ctgcacgaag ttgagctgct tgttgccttg
     3061 tcgatgcggg cgatgcgacc agccagcgac gacagatagt cgtcattggt gccgcccgac
     3121 gcaacgtgtt cgagcagtgc cttgagcggc acgctcgggt tctgttcgag cggcctggta
     3181 tcggccagct tggcttcgct gacaccgacg cagtcgacaa tcagaaagtg ggtctttgcc
     3241 ttggcatcgg gcgtcaccga gcgcagctcg tcggggctga caacccggac gccgcggcct
     3301 ttcatctgct cgaacagcac gcgcgacttc accgtgcgca gaaacatcac gatttcgacg
     3361 ggctttatgt cggttccggt cgcgatgaga tcgacggtga cggcaatgcg agggttgtag
     3421 ctggtgcgga agctctggat cagatccgcg ggctttgtgc cggtgacttt gtaggtaatc
     3481 ttcgtcgcga agtcgttgcc ttggccaaac tcttcgcgaa cgatgcgcac gatgtcttcg
     3541 gcgtggctgt cgtctttggc gaaaatcagc gtcttgggga cgtgggtgcg ctcggggaac
     3601 gtgtctttga gcacttggtc gcggagggtg cggatgatca gccggatttg atcaatggcg
     3661 acgacgttgc ggtcaagatc cttcgcttcg tagctgacat cttcgtcggg cgcttcccag
     3721 cgggtcttgc gcgtcagccg gtcgcgatag ccgagcatca cgccggggct tgcctcgacg
     3781 gtggaaccct gcgcggtgat ctgggtgcgg attttgtaga cgtcgaagtt gacgttggca
     3841 ccctcggcga cggcgcgctc gtgtgggtat tccatcacga cgttttggtg aaaaaatccg
     3901 taggtgtgct tggccggtgt cgccgtcagc ccgagcaggt gcgcgtcgaa atactccagc
     3961 acctgacgcc agagcgagta gatgctgcgg tggcactcgt cgatgaagat gatgtcgaaa
     4021 aactcgggag ggatcgcttg gttgtagacg acgggcagcg gttctttgac cgtcacatcg
     4081 tcgctgtcaa aactggtgtg cgcttcggcc tcggggtcta gctcgggctc tcccttgagc
     4141 accgaataca gccgctgaat cgtgctgatg accactttgc tcgacgagcc cagcgtattc
     4201 gaggtcagcc gctgcacggt atacagctcg gtaaacttgc gctggtcgtc gggcgtgcgg
     4261 aagctctgaa actctttttc ggcctgctcc cccaggtttg cgcggtcaac gagaaacagc
     4321 acccgccgcg ccccaccaaa cttgatcagc cgatacagcg tcgtcaccgc gagcagggtc
     4381 ttgccactgc ccgtcgccat ttggatcagc gagcgtggac ggtcgtcaaa caggctcttt
     4441 tcaatccgag tgaccgcctc gaccttgttc ggccacatgc ccggcaagac aaccggcggc
     4501 atcgcccgca gtcgcgcccg cagcgtcgat ggcttggtat cgtcggacgc cgtgtagtga
     4561 ccgccgcgtc ggacccatgt atcgagcgta tcggcggaca gccactcacg catggtgtcc
     4621 ggcctgtgga agctccagac ctcgcgcgtc cgaggccgtg ggtccaacaa gttgataaac
     4681 gccgtctctt cccccgtcga gatgtacgca aacggcagag gcttgtgcgg cgcgtcgaga
     4741 tcgggcggca gtccctcgac atacttctgc gtctgcacct cgacgttgcg aaccggcaca
     4801 cctgcctttt tggcttcaca gaccccgacg gccactccat cgacaaacag caaataatca
     4861 gcgaagccat gcccacgcga aagcttgaac tcacgcaccg ccaccgccgg cagcgtcagg
     4921 ttcatctcgc catagtcctg caccgtccag cccgactgca caagcagtgc gtcgatcttt
     4981 tcccgtgccc ggttttcagg cgtgttcttg attcctgccg atgacatgcc gattcccccc
     5041 ggaaatacca aagactccac gacgatcgta agagcaaatg tgcaaattcc gcacacccaa
     5101 tgccgaccga gcggcagtcc ttcgcgcccg gcctacttcg actgtcccgc cgccgctcgc
     5161 tgcgcttccc gatccgcgt
//
LOCUS       DAORGN010000281         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8214_length_5138_cov_5.355499, whole genome shotgun sequence.
ACCESSION   DAORGN010000281 DAORGN010000000
VERSION     DAORGN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8214_length_5138_cov_5.355499"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1639)
                     /locus_tag="PKL17_20210"
     CDS             complement(293..1639)
                     /locus_tag="PKL17_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47118.1"
                     /translation="MTFRLSRPSSTSITAAGFAPGSLMRSLTCAAWLCGAIGTAEAKD
                     APTAEHKAFEDQLRQMAPAAAPAPTNSLGTIGLHSKTSKVPASLEFSDALEEGALGTS
                     EMRKANRVAAEILQKRMEETRPTDFRLIREAAGADVVVLSGVYDRVQDVLRAAQIKHV
                     VVPPQLLDQMELLSTQTVMLNCPGNVSDAGVKKLRDFVARGGYLVSTDWALRTVEKAF
                     PGTIGHNGRTTPNDVVSVQVHDDHEPLLRHVQVMKDHPRWWLEISSYPIRVKDPKNVR
                     VLMSSQEMAKKYGDGAMVVSFHHQQGKVLHMTSHFFLQQSKLSSDSEKQKGSSFAKGA
                     GLSDKQLGELAKKGVAIDDVKAGELNAAYSMQQVSTNLIAEKQAQNEDLLRKQYSAKV
                     KADDVALGSGEGQTPSAKSPRLKKDFRVQVLDRKGKQAKVRDLFGHEGWVDADALY"
     gene            1862..2686
                     /locus_tag="PKL17_20215"
     CDS             1862..2686
                     /locus_tag="PKL17_20215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNK47119.1"
                     /translation="MQTIGGGLLDEKVTDWPGVVIDTETTGVSVQTDRVVELGAIFCL
                     RGSVVDSRKMRLNPQMKIPAEASAVHGICDEHVAGKPTFAQIADRFVDYITGRARQDV
                     TPWLCGYNAVEFDGPLLNAELLRAGIDVQIDVSQIVDPITFVRWHLRHLRSRSLKSAC
                     EHFGVPLENAHSALADAQATLRLLHSMVEHRYIPTRLGDILSEQARLGRIMKEEWDRW
                     SYWLYRDREDGSLRFGAGSHCGQPVRDVGSDYIESLLRKVPDLPDEVRTQLRAHAA"
     gene            2834..4297
                     /locus_tag="PKL17_20220"
     CDS             2834..4297
                     /locus_tag="PKL17_20220"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HNK47120.1"
                     /translation="MSAQQSIPWEQQALHIAIDTIQEAMPGKPKEQQEALHMSLMTLR
                     GFLAAQSSVDEDLPPPPLQVQSDAHRSNKFKQLLQERRTMAGLSTSRLAELAGLPDSA
                     IANIESGRALPTQLALLHLLSVPELKLEVSDLPWEAAETDPNFALNCWLAPGFDPVKM
                     IKELSMQVNSFGGHIEQGYLYMDPMSAADWCAIADLKDYDEMQSSMQLDKSAAVVLEQ
                     CGKMGLDVVALGPGSARNEVRLVEQLLAQGGHGDVRLYLLDISQPLLSVAYRHAAETL
                     ADRGGVVFAIQGNFHHLPRYGQLLYSPQRAHRRRLVTMLGHTFSNLENELRFVRNSLV
                     GFGAGDLLLLDIRLAYASTDRPAEEIIEKDPGLLKSRRNPNSALRQRWDDFLAGPIRR
                     YANVQTEIDVQRVLDLSCPIPGSYAVDARARVRLPSGEEREFSVYYSRRYDSQKLQAA
                     LFREGWETIANWAYGGDVNPCMLFLLRKSGPPLRLVE"
     gene            4308..4910
                     /locus_tag="PKL17_20225"
     CDS             4308..4910
                     /locus_tag="PKL17_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47121.1"
                     /translation="MRLRRAACALLAFLALSAAPLRAEAGPGSRWFLEHLALDVAAVT
                     SWRVAWADERNPGFNVLGGGGELHVGLEFDSGVGFLLGTRAVFGPNRSVVEGQPYGDV
                     TGDALLLFRLTDWVRVAAGAAGGRLWMCCGNQSVPAQTDAVILGGLLRIGIDFYPRQS
                     NVLKALSLWLRIDIDGHPGSSESLLPTTSLSMSGSFGFRL"
     gene            complement(4918..>5138)
                     /locus_tag="PKL17_20230"
     CDS             complement(4918..>5138)
                     /locus_tag="PKL17_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47122.1"
                     /translation="TKSRRRGLGSLSKHAFSPVAAPLHLVTKPRPAIRAELPSEGLPA
                     ALPADAVGFLRAELAAMLALLEEPTGST"
BASE COUNT          896 a         1572 c         1581 g         1089 t
ORIGIN      
        1 cgaaggccca cccatcctgc gcccagcagc ccgtggcgca gcacctcccg tctcgtctgg
       61 ctcatgtctg ccctcctgca cccggttccc ccgaacacgg tgcgggtctt gtcttgcttg
      121 tcgttcgacg attggacgcc cggcgtcttg gagtctgcat gcaagcgaag agcaaagtcg
      181 tagggcttta ttttaccgct acacgtcaca aatcagacga gcgatcggct gctctcgcgc
      241 atttcttcca catgggcagg tcgcacaaac cacgcacctg tgctacgcgg ccctagtaca
      301 gcgcgtctgc atcgacccag ccttcatggc caaacaggtc gcgcaccttg gcttgcttgc
      361 ctttgcgatc caggacctgc acgcggaagt ctttcttcag ccgaggactc ttggcgctgg
      421 gcgtctgccc ttcaccgcta cctagcgcca catcgtccgc cttcaccttg gcgctgtact
      481 gcttgcggag caggtcttcg ttttgcgcct gcttttccgc gatcaggttg gtggacacct
      541 gctgcatcga gtacgccgcg ttgagctctc ctgctttgac gtcgtcgatg gctacgcctt
      601 ttttcgccag ctcaccaagc tgcttgtctg acagacctgc gcctttggca aagctcgatc
      661 ccttctgctt ttcgctgtcc gagctgagct tggactgctg caaaaagaag tggctggtca
      721 tgtgcagcac cttgccttgc tggtgatgaa acgacacgac catggctccg tcgccgtact
      781 tcttggccat ctcttgcgac gacatcagca cccgcacgtt cttcggatct ttgacccgaa
      841 tcggatacga cgagatctcc agccaccagc gcggatgatc cttcatcact tggacgtgac
      901 ggagcagcgg ctcgtgatca tcgtgaacct ggaccgacac cacgtcattg ggtgtcgtgc
      961 gaccgttgtg accgatcgtg ccgggaaacg ctttttcgac cgtgcgcagc gcccagtccg
     1021 tcgataccaa gtagccaccg cgtgccacaa agtcgcgcag cttcttgacg cccgcatcgc
     1081 tgacgttgcc aggacagttg agcatcaccg tctgtgtgga cagaagctcc atctgatcga
     1141 gcagctgcgg cggcaccacc acatgcttga tctgcgcagc ccgcagcaca tcctgcaccc
     1201 gatcgtagac gcccgacagc accaccacgt cggcacccgc ggcttcgcga atcagtcgaa
     1261 agtccgtcgg tcgcgtctct tccattcgct tttgtaggat ctctgcggcg acgcgattgg
     1321 ccttgcgcat ctcgctggtt ccgagcgctc cttcttccag tgcgtcggaa aactcgaggc
     1381 tggcgggaac cttgcttgtt ttgctgtgta gtccgatcgt gccgagtgag ttcgtcgggg
     1441 ctggagccgc ggctggcgcc atttggcgaa gctgatcttc aaaggctttg tgctcagcag
     1501 tgggagcatc cttggcttcg gcagtcccga tcgcgccaca cagccaggcg gcgcaggtga
     1561 gcgagcgcat caaagaaccc ggtgcaaagc cagcagcagt gatggaagtg gatgacggac
     1621 gggaaaggcg gaatgtcacg ggcggcctcc tcaagtcttc tgcgcatccc aaacgcccgg
     1681 tcacttgctt cgatctatct gcggatcggc cagcgcgaag gcgagacaac gggcagaccg
     1741 gcagatcggc ggactctgca ctcgcggcac tgggacttgt ttcgcgagtc actgttcgct
     1801 tggcatgcgt cgcaacttgc gttgacagcg ccgacaagtt gtggcgtaaa ctgcccgact
     1861 gatgcagacc atcggcggtg gactcctcga cgagaaagtg accgactggc cgggtgtggt
     1921 gattgacacc gaaaccaccg gcgtatcggt ccaaaccgac cgcgtcgtcg aactgggcgc
     1981 gatcttctgt ttgcgcggca gcgtcgtcga ttcacgcaaa atgcggctca atccgcagat
     2041 gaaaattccc gccgaagcca gcgcggttca cggcatctgc gatgaacacg tcgcaggcaa
     2101 gccgaccttt gcgcagatcg ccgatcgctt tgtcgattac atcactgggc gtgctcggca
     2161 ggacgtgacg ccgtggctgt gcggctacaa cgcggttgaa ttcgacggac cgctgctcaa
     2221 tgccgagctt ttgcgtgccg gcatcgacgt acagattgat gtcagccaga tcgttgatcc
     2281 cattaccttt gtgcgttggc atctgcgcca cttgcggagt cgatcgctca agagtgcgtg
     2341 cgagcacttt ggcgtgccgc tggagaatgc acactcggcg ctggccgatg cacaagcgac
     2401 gcttcggctg ctccattcga tggtggaaca tcgctacatt ccgacgcggc tgggcgacat
     2461 cttgagcgaa caagcccgtc tgggccggat catgaaagaa gagtgggatc gctggtcgta
     2521 ctggctgtat cgcgatcgcg aagatggctc gctccgcttt ggcgccggtt cccactgtgg
     2581 gcagccggtg cgcgacgtcg gcagcgatta catcgaaagc ctgctgcgca aagttcctga
     2641 tttacccgac gaagtccgca cccagctgcg tgcacacgcg gcctagctgt tttttgcctg
     2701 gcggctgggc ctgctgattt cgtgtactga aacaggccca actgaactat agtgtgggag
     2761 agtttggcac cgtctcgtcg agtcatccga ccaatccctc cgcttggtgc cattgccgta
     2821 tttcgaggtg tccatgtccg cgcagcagag cattccctgg gagcaacagg ccttgcacat
     2881 cgccatcgac accatccaag aggccatgcc gggcaagccc aaagagcagc aagaagccct
     2941 tcacatgagc ctgatgacgc tgcggggttt tctggccgcg cagagttctg tcgatgagga
     3001 tctgccgcca ccgccgctgc aagtccagtc tgacgcccac cggagcaaca agttcaaaca
     3061 gcttcttcag gagcggcgca cgatggcggg tctatcgacg tcgcggctgg cggaactggc
     3121 cgggctgcct gactcggcga ttgctaacat cgagtccggg cgggcgctgc cgacccagct
     3181 tgcgctgttg cacctcttgt cggtgccgga actgaagctc gaggtgtcgg atctgccatg
     3241 ggaagcggct gaaaccgatc ccaacttcgc gctcaactgc tggctcgcgc cggggtttga
     3301 cccggtcaaa atgatcaaag agctgtcgat gcaggtgaac agcttcggcg gtcatatcga
     3361 gcagggctac ctttatatgg acccgatgag tgcggcggac tggtgcgcga tcgccgacct
     3421 caaagactac gacgagatgc agtcgagcat gcagctcgac aaatccgcgg cagtggtgct
     3481 ggaacagtgc ggcaagatgg ggctcgacgt ggtggcactt ggccctggca gcgcgcgcaa
     3541 cgaggttcgt ctcgtcgaac agcttctggc gcagggcgga catggcgatg tgcggctcta
     3601 tctgctcgac atcagccagc ctcttttgtc ggtcgcgtat cgtcatgccg ccgagacgct
     3661 ggcggatcgc ggtggcgtgg tgtttgcgat ccagggcaat ttccaccacc tgccgcgtta
     3721 cggccagctc ttgtattcgc cccagcgagc gcaccggcga cggctggtca cgatgcttgg
     3781 ccacacattc agcaatctcg aaaacgagct gcgctttgtg cgcaacagct tggttggctt
     3841 tggcgccggg gatctgctgc tgctcgacat tcggctggcc tacgcatcga cggaccgacc
     3901 cgccgaagag atcatcgaaa aagatccggg gcttttgaaa tcccgtcgca atcccaactc
     3961 ggcgcttagg cagcgctggg atgattttct ggctgggccg atccgccgct acgccaacgt
     4021 gcagacagaa atcgacgtgc agcgtgtgct cgacctgtca tgtccgattc cgggcagcta
     4081 tgcggtcgac gcgcgggctc gggtccggct gccttccggc gaggagcgcg agttctcggt
     4141 gtactatagc cgtcgctatg attcgcaaaa gctccaggcg gcgctgttcc gtgaaggctg
     4201 ggagaccatc gccaactggg cttacggcgg cgacgtcaac ccctgtatgc tcttcctgct
     4261 gcgcaagtcc ggtcctccgc tgcggctcgt cgagtaggtg gcgatcggtg cggcttcgac
     4321 gtgctgcctg cgcactgctt gcgtttttgg cgctcagcgc tgccccactg cgggccgagg
     4381 cgggaccggg cagccgctgg tttctcgaac acctggcact cgatgtcgca gccgtgacgt
     4441 cgtggcgggt tgcttgggcc gacgagcgca atcctggctt taacgtgctc ggtggtggcg
     4501 gtgagctgca tgttggcttg gagtttgaca gtggcgtcgg ctttctgctt ggcacgcgcg
     4561 ccgtctttgg cccaaaccgc agcgtcgttg aaggccagcc ctatggcgat gtcacgggcg
     4621 acgccttgct tttgtttcgc ttgaccgact gggtgcgtgt cgcggcgggg gcggcggggg
     4681 gccggctgtg gatgtgttgt ggcaatcagt cagtgccggc tcagaccgac gcggtgatcc
     4741 ttggcggact tttgcgcatt ggcatcgatt tttatccgcg gcagtcgaac gtgctcaagg
     4801 cgctgtcttt gtggctgcgg attgatatcg acggacatcc ggggagcagc gaatcacttt
     4861 tgcccactac cagcttgagc atgtccggca gctttggttt tcgcctgtag acctgggcta
     4921 cgtcgatccc gtcggttcct caagcagcgc aagcatcgcc gccagctcgg cgcgcaaaaa
     4981 accgacagca tccgcaggca gtgcggcggg cagtccttcg ctcggcagct cagcgcggat
     5041 cgctggacgc ggcttggtga ccaagtggag tggggccgcc accggcgaaa acgcatgctt
     5101 tgacagggaa cccagaccgc gtcgtcgact tttcgtgg
//
LOCUS       DAORGN010000282         5116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8272_length_5116_cov_6.942501, whole genome shotgun sequence.
ACCESSION   DAORGN010000282 DAORGN010000000
VERSION     DAORGN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_5116_cov_6.942501"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKL17_20235"
     CDS             <1..443
                     /locus_tag="PKL17_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47123.1"
                     /translation="VRHAATSFALAELQRRGPVQTLVIDDTYLPKEWTDSQTLRESAH
                     IPYQGRLYTGLQHHLSSVICTYAFVVAERERQERNGNPGKGVMVRTAEIPQRHSPTSI
                     ATLRKRIANAVTPWFLAAFPARPRRQTTQTQTDVDRALTKGPGG"
     gene            573..1700
                     /locus_tag="PKL17_20240"
     CDS             573..1700
                     /locus_tag="PKL17_20240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNK47124.1"
                     /translation="MILIVGGLAGIKFRQISMLMQAGKEDAKQGPPPESVSTTVVKED
                     SWEDILPAVGSVVAAKGVSISSEVPGAVTAIHFESGAFVKAGQVLVELDSSIERAQRA
                     SAMARRELAQLSAGRARQLAKQSAISRSQLDGDEAQLKTSRADLSALQAQIDRKTVRA
                     PFSGRLGIRAVNLGQYVNPGTMLTTLESLEVVYVDFSVPQQELAEVVPKTPVRVSLSG
                     DSGPTFEGVIAAVDPAVDATTRSVKLRASIPNPKEQLRPGMFVRVAVVLPKRQQVVAA
                     PATAVVHASYGDSVFVVEDKKDESGVLIKNPDGQPAKVARQQFVRLGVSRGDFVALLD
                     GVKAGQELVTAGAFKLRNGMGIAIHNEVQSSFSLTPNPGNR"
     gene            1707..4763
                     /locus_tag="PKL17_20245"
     CDS             1707..4763
                     /locus_tag="PKL17_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNK47125.1"
                     /translation="MKFTDIFVRRPVLAMVVNLVIIIAGLQAVGSLNVRQYPKLESAT
                     VTVRTAYVGASADLVRGFITTPLERAIAAADGIDYIESQSAQSLSTINVRLKLNTSAA
                     AALADISTRVNQIRADLPPEAEVPTIAIETSDSMIASMYLSFGSTILDPNQLTDYLIR
                     VVQPRLSALAGVQKADILGGRTFAIRAWLKPDRMAALNVSPSQVRQALAANNYLSAVG
                     QTKGALIQLNLTAATDLRSVEDFRRLVIRQQGGTLLRLSDVADVVLGADSYEQDVRFS
                     GKQALFMGVWVLPNANALDVIKRVRAEIASIQRELPTGVTAEVAFDATKYITSAISEV
                     QKTLFETVLIVIVVIFLFLGSLRTVLVPLVAIPVSLVGAVFLMQAFGFTLNLLTLLAV
                     VLAVGLVVDDAIVVVENVERHIREGKTPTEAALLGARELVGPIVAMTITLGAVYAPIG
                     FQGGLTGALFREFAFTLAGAVLISGVVALTLSPMMSSRLLKANHAPGLLARLIDRGFA
                     RLMHGYEQLLSATLRARRAVYLVWIVLTLMLVPMYLFSPSELAPNEDQGVVFGALDVP
                     PNATLEQLTPYTEQMERYFEETKEFDHSFQITFPSGGFGGVIVQPWDKRKRNIFAIQG
                     ELFGKTSQITGIQAPMFLPSALPSAGTFPVELVLASTAGHEELLRIADRLVQEATKSG
                     LFAFPPMLDVRIDQAKTEIVIDRDKVASMGLSMQQVGADLSAMLSGNFVNRFNIDGRS
                     YKVIPQIERAGRLTADQLKEIHIAGPAGQVIPLSAVASLKHSVEPRTLNRFQQLNAVK
                     ISGLAPRSLEQGLRFLEDAAAQILPPGYRIDYTGESRQLREEGGKFLPAMALALILIF
                     LVLAAQFNSFRDPFVILAGSVPLALFGALSFTFLKFAGPPGTAFPLTEGWTTTLNIYS
                     QVGLVTLVGLIAKNGILIVEFANVRKEEGLSKLAAVQQAAVTRLRPILMTTVATVIGH
                     FPLTLVTGAGAVARNSIGIVLVGGMTIGTLFTLFIVPSLYVLLAKD"
     gene            complement(4770..>5116)
                     /locus_tag="PKL17_20250"
     CDS             complement(4770..>5116)
                     /locus_tag="PKL17_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47126.1"
                     /translation="RDSSSTSLPAPSLYSGVKNEAFWQSSTGSSTKPARSFPILSSSH
                     SETVTSCLAMVTSYLSFVQYPAQRLQERLIGIVHSQVIRIDFGGAGEIVARIAVQSFE
                     RSLIIQRCIDGA"
BASE COUNT          980 a         1377 c         1720 g         1039 t
ORIGIN      
        1 acgttcgtca cgcagcgact tccttcgcgc tcgccgagct tcagcgtcgc ggtccagttc
       61 agacgcttgt catcgacgat acgtacctcc caaaggaatg gacggactcc caaacgctgc
      121 gggaaagcgc gcatattccg tatcaaggga gactgtacac gggactgcaa catcacctaa
      181 gttcggtgat ctgcacgtat gcgtttgtcg tcgcagaacg agagcgccaa gagcgaaatg
      241 ggaatccagg aaaaggagtg atggtgcgca ccgcagagat tccgcagcgg cacagtccga
      301 cctccatcgc gacgctgcgc aaacgaatcg cgaacgccgt aacgccctgg tttctcgccg
      361 catttcccgc ccgtcctcgt cgccagacca ctcagacaca gacagatgtg gatcgagcct
      421 taacgaaagg acctggtgga tgacgcagtt gtggtaggta gtcttttttc gcgggcttgc
      481 tggacgcttc acattttttg cggactcgac agtagttggg caagcggcgg aatccaaagg
      541 acatcatgcg ctacgtcaaa gcagtcgctc ttttgattct tattgtcggg ggtcttgcgg
      601 ggatcaagtt ccgtcagatc tcgatgctga tgcaggcagg caaggaagat gccaagcagg
      661 ggccgccacc agagtcggtg agcacgacgg tggtcaaaga agactcctgg gaggatatct
      721 tgccggcggt gggcagtgtg gttgcggcca agggggtgtc gatcagcagc gaggtgccag
      781 gggcagtgac cgcgattcac tttgagtcgg gggcgttcgt caaggcgggg caggtgctcg
      841 tcgagctgga cagcagcatc gagcgagccc agcgggcctc ggcgatggcg cggcgcgagc
      901 tggcgcagct gagtgcgggt cgagcgcggc agcttgcaaa gcaatcggcg atttcgcgtt
      961 cgcagcttga tggggatgag gcgcagctga agacctcgcg ggccgacctg agcgcgctgc
     1021 aagcgcagat cgatcgcaag acggtgcggg cgccgttttc gggtcggctg ggcattcggg
     1081 cggtaaacct gggtcagtac gtcaatcccg gcacgatgct gacgacgcta gagtcgctgg
     1141 aggtcgtgta cgttgacttc tctgtacctc agcaagaact ggccgaagtg gtgccgaaga
     1201 ctccggtgcg ggtgtcgctg tctggggatt cggggccgac gttcgaaggg gtgattgcgg
     1261 ctgtggatcc ggcggtcgat gccaccactc ggtcggtgaa gctgcgggcg agcattccca
     1321 acccaaagga acagctgcgg cccggaatgt ttgtgcgggt ggcggtggta ttgccgaagc
     1381 gccaacaggt ggtggcggcg ccggctacag ccgtggtgca tgcctcgtat ggggactcgg
     1441 tgtttgtggt cgaagacaag aaggacgaga gcggggtgct catcaagaat ccagacggcc
     1501 aacccgcgaa agtggcacgg cagcagtttg tgcgattggg ggtttcacgc ggcgactttg
     1561 tggcactgct cgacggggtg aaggctggtc aggaactggt gacagcaggc gcgttcaagc
     1621 tgcgcaacgg catgggaatc gccatccaca acgaggtgca gtcgagtttt tcgctgactc
     1681 ccaacccggg aaatcggtaa tcggccatga agttcaccga catttttgtg cggcgcccgg
     1741 tgctggcgat ggtggtcaat ctggtgatca tcattgccgg actgcaggcg gttggctcgc
     1801 tcaacgtccg gcagtatccg aaactagaga gtgcgaccgt gacggtacgg acggcatacg
     1861 ttggggcgag cgcggacctt gtgcgggggt ttattacgac gccccttgag cgggcgattg
     1921 cggcggcaga tggcattgac tacatcgagt cgcagagcgc ccagagccta tcgaccatca
     1981 acgtgcggct caagctcaac acgagcgccg ccgccgcgct tgcggacatc agtacccgcg
     2041 tcaatcagat ccgggcggac cttccaccgg aagcggaagt accgacgatt gccatcgaga
     2101 cttcggattc catgattgcg tcgatgtacc taagctttgg ctcgacgatc ttggacccga
     2161 atcagcttac ggactacctc atccgcgtgg tgcagccgcg gctgtcggcc ctggcgggcg
     2221 tgcagaaagc ggacattctg ggcgggcgga cgtttgcgat tcgggcatgg ctcaagccgg
     2281 atcggatggc ggcgctgaac gtaagcccct cgcaggtgcg gcaggcgctg gcggcgaaca
     2341 actatctgtc ggcagtgggg cagaccaaag gggcgcttat ccagctgaac ctgacggcgg
     2401 caaccgacct gcgttcggtc gaggactttc ggcgtctggt gattcgtcag cagggcggga
     2461 cgctgctgcg gctgtccgat gtggccgacg tcgtgctggg cgcagattcc tacgagcaag
     2521 acgtgcgctt ttcgggcaaa caggctctgt ttatgggggt gtgggtgctg cccaacgcaa
     2581 acgcactcga tgtgataaag cgggtgcggg cagagattgc gtccatccag cgcgagctgc
     2641 cgacgggtgt gacggcagag gtggcgttcg atgcgaccaa gtacatcacg agcgccatat
     2701 ccgaggtgca aaagacgctg tttgagactg tgctgatcgt gatcgtggtg atctttctgt
     2761 tccttggttc gctgcggacg gtgctggttc cgctggtcgc gattcccgtg tcgctggtgg
     2821 gggccgtgtt cctgatgcag gcgttcggtt ttacgctcaa cctgctgacg ctgctggcgg
     2881 tggtgctggc ggtgggactg gtggtggatg acgcgattgt ggtggtcgaa aacgtcgagc
     2941 ggcacattcg cgagggcaaa acaccgacgg aagctgcact actgggggcg cgtgaactgg
     3001 tcggtccgat tgtggcgatg acaattacgc tcggtgcggt gtatgcgccg attggctttc
     3061 aggggggact gacgggtgcg ctgtttcgcg agtttgcttt tacactggcg ggcgcggtgc
     3121 tgatctcggg ggtcgtggcg ctgacactgt cgccgatgat gtcctcgcga ctgctcaagg
     3181 caaaccacgc accggggctc ttggcgcggc ttatcgaccg agggtttgcg cggcttatgc
     3241 acggctacga gcagcttctt tctgcaacgc tgcgggctcg gcgggcggtg tacttggtgt
     3301 ggatcgtact tacgctcatg ctcgtgccga tgtatctgtt ttccccaagc gagcttgcgc
     3361 caaacgagga tcaaggggtg gtgtttgggg cactcgatgt cccgccgaac gcgacgctgg
     3421 agcagcttac gccgtacacc gaacagatgg agcgctactt cgaggaaacc aaggagtttg
     3481 atcacagctt tcagatcacg ttcccatcgg gaggttttgg cggcgtgatc gtgcagccgt
     3541 gggacaagcg caagcgcaac atctttgcga ttcaaggcga gctgtttggc aagacctcgc
     3601 agattacggg aatccaggcg ccgatgtttc tgccgtcggc gcttccgagc gcgggaacat
     3661 tcccagtcga actggtgctg gcatcgaccg cggggcatga agagctgctg cggattgcgg
     3721 atcgcctggt gcaggaagcg accaagagcg ggctgtttgc gttccctccg atgctcgatg
     3781 tgcgcatcga tcaggccaag accgagattg tgatcgaccg cgacaaagtg gcatcgatgg
     3841 ggcttagcat gcagcaggtc ggcgcggact tgtcagccat gctgagcggc aactttgtga
     3901 atcgcttcaa catcgatggc cgcagctaca aggtcattcc gcagatcgag cgggccggaa
     3961 gactgacggc ggatcagctc aaggaaatcc acatcgccgg acccgcagga caggtgattc
     4021 cactttcggc ggttgcgtcg ctaaaacaca gcgtcgagcc gcggacgctc aatcgctttc
     4081 agcagctcaa tgcggtgaag atatcggggc tggccccgcg gtcgttggag cagggcctgc
     4141 gctttttgga agacgcggca gcgcagatac ttccccccgg ctatcgcatc gactacaccg
     4201 gagagtcacg gcagctgcga gaggaaggcg gcaagttctt gcccgcgatg gcgctcgcac
     4261 tgatcctgat ctttttggtg ctggcagcgc agtttaactc gttccgcgat ccgtttgtga
     4321 tcctggccgg ctcggtgccg ctggcgctgt ttggtgcgct gtcgtttaca ttcctaaagt
     4381 ttgccgggcc gccgggaacg gcgtttccgc ttacagaggg atggacgaca acgctgaaca
     4441 tctactcgca ggtcggtctg gtgacgctcg tcggactgat cgcgaaaaat ggaatcctga
     4501 ttgtcgagtt tgcaaacgtg cgcaaagagg aaggactgag caagctcgcc gccgtacagc
     4561 aggccgccgt gacccgactg cgtcccatct tgatgaccac ggtggcaacc gtgattggcc
     4621 acttcccgct gacgctggta acgggagcag gggcggtggc gcggaactcg attgggattg
     4681 tacttgtggg tggaatgacg attgggacgc tctttacgct ctttatcgtg ccatccctgt
     4741 atgtcctgct ggcaaaggac tgacgcttgc tacgcgccgt cgatgcaacg ctggatgatc
     4801 aggctgcgct cgaatgattg caccgcgatg cgcgccacga tctctcctgc tccgccgaaa
     4861 tcgatccgga tcacttggga atggacgatt ccgatcagtc tttcttgtaa acgctgagct
     4921 ggatactgga cgaaagacag gtaggatgtc accatagcca agcacgaagt taccgtctcc
     4981 gagtgcgacg agctgaggat aggaaaggaa cgtgccggct ttgtcgacga cccagttgac
     5041 gactgccaga acgcttcgtt tttcacgccg gagtacaaag aaggcgcagg aagactggta
     5101 ctcgacgaat cgcggc
//
LOCUS       DAORGN010000283         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8587_length_5004_cov_5.353809, whole genome shotgun sequence.
ACCESSION   DAORGN010000283 DAORGN010000000
VERSION     DAORGN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8587_length_5004_cov_5.353809"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PKL17_20255"
     CDS             <1..151
                     /locus_tag="PKL17_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK47127.1"
                     /translation="YRALTVYMTSSTNYKAARTATLNAAKDLYGSAGAEYAAVGNVWT
                     AVGVN"
     gene            539..1339
                     /locus_tag="PKL17_20260"
     CDS             539..1339
                     /locus_tag="PKL17_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47128.1"
                     /translation="MEPSVLLLVEGRDDALMARWTLKAAQYPLGRLTIRAVGGKYRIA
                     SELKKLGAEAGRCAALLDLDERSVPDAVARARDQLGQPTVEVFCAIPAIEAWLFADDN
                     LALSQASLDEETASVLRRIPLPEEIPNPKELARVLFGPPVNWAFMQNMDVQRASARCP
                     SLRAFLTGMGRLLGVSTQPIEDSVSRSLSRNVFAGLLAEIVPSDAIAWRTASGETLTA
                     AALRKEIESGTELGHQYASDLLRVSRDFLRRIASRAESRTDPSIKASK"
     gene            1336..1980
                     /locus_tag="PKL17_20265"
     CDS             1336..1980
                     /locus_tag="PKL17_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47129.1"
                     /translation="MILFAPSYDPATGANLAVAQEVATTACQCLFQEAATAEALHTAL
                     IQSPQPLFAWSHGRPAQLLAQHGHTAISEPVLAVLGARQVFAFACHTATRLGQLASQQ
                     GTVWWGYTGAIQCPDDSPAVLPLFVSIFKFLREEFSTADTTDLRSVVISSLAKMCEAA
                     AFSIDDLAASNPTMVVHEALLCLHHLWDRLRVWAPDGAFPEHHPAASPPSLFAD"
     gene            complement(2059..2907)
                     /locus_tag="PKL17_20270"
     CDS             complement(2059..2907)
                     /locus_tag="PKL17_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47130.1"
                     /translation="MSIRFVSMGTLGLPLFALALGCSHTPTHSGPVPSSETLIPQVDA
                     QALKAEPAGQLVGPHFLSATTEEDGRTDWNLPLQEGLCYTFSGIGGQGVKQLYLYLWD
                     QAEKRVATEKPERPQVTLRYCPDVSGSYHLQAKTGEGFGPMAVGVYAVAAPPKAAPPP
                     PPVAPPPLDLGPIIDQQAAAAAPGAVRVGDFYRGVAGGGNDRSDWFTTLEPGRCYWFI
                     GAGAPTVKELYLYLWNPQLQRVSDNRASSNRTMMGHCPTVPGMYKIEAKVNSGKGDYQ
                     LGVYVK"
     gene            complement(3048..3839)
                     /locus_tag="PKL17_20275"
     CDS             complement(3048..3839)
                     /locus_tag="PKL17_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47131.1"
                     /translation="MVSGGYFCTAAHTETGGIHDFLQKLDPKVKWERCFPAVRKPAPK
                     LHRPHPVPVSGQSGASGQYLVDTMLELLAKHYHGAACSFDFVVLIDDADCRFAGAPDV
                     TAAFLAWEADLTEQVRAATGKPTLAFHALLAWPEIEAWLLADWNHSFAKHYRPIAHAL
                     RKHVETCILAPLTFAQIDQFGGRLQNGSCEHKLSSALQASFTAIDPCPCKLADIRRNQ
                     PETAVLRYSKQVDGPLLLKSIEPDRVAQASARFRSAQTRLRVAGG"
     gene            complement(3833..>5004)
                     /locus_tag="PKL17_20280"
     CDS             complement(3833..>5004)
                     /locus_tag="PKL17_20280"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNK47132.1"
                     /translation="TSFLQTVEKHGRAEVVNRTHGSKTRTRRLAFQWLLQSVRQPSQV
                     ESILKVQPSPRDLLYALSYVVGDSADFPMGFHVEIEELLEFAHAPIIDRRWSSARGKL
                     LARRGGRRADQCEFGLVTHGKEPKPKLTPTLVVDVDTNETILKQSAKLLKDKQFYEHV
                     YPEFSKTQDELLAFARGFHSYASTDLDVRQLAEGAKIDLSVRALDAEGSQFVNVLRYL
                     DQKYDFLDEYAERLRELCPDLRKLKVIDASDTYKQLELNIGGHKYKPREMSDGTLKAM
                     WLALLLFSPEKGSVLSIDEPEMNLHPAWLKVIGRWLQRFSSAEQLFVSTHSPDLLDTF
                     TEGFKTGEVALFVFGLGDKEMRRVEPGELESFWKDGWELGDLYRVGEPKLGGWPW"
BASE COUNT          900 a         1497 c         1540 g         1067 t
ORIGIN      
        1 gtaccgcgcg ctcacggtct acatgacgtc gagcaccaac tacaaggcgg cgcgcacggc
       61 gacgctcaat gcggcaaagg atctgtatgg gtctgccggg gcggagtatg cggcggtggg
      121 gaatgtttgg acggcggtgg gagtgaacta gcgcttcgtt gagggagaaa cctaaaaacg
      181 gtttctccct cggaccctct tccctttctt cagagccttc cttttttctt ctccccccat
      241 ccccctccgg ggcgacccct ccctaaccct ccccgtcggg ggagggaacg cgctttgtct
      301 tttgcttggc ttcgccttcg cggtttgatg ggtcgtcttg gcttgccttc ggctttgctc
      361 tggcttgcct tcggcttcgc tcttggctcg ccttcggctt tgctctggct tgccttcggc
      421 tttgctcttg gcttgccttc ggctttgctc ttggcttgcc ttgagatggt ggccggtgca
      481 tggtacggtc cgaaatatgg tgtcggctac gtctgaaacg ggaaatactg aggcacagat
      541 ggagccgtcg gttttgttgt tggttgaggg ccgcgatgac gctttgatgg ctcggtggac
      601 tctcaaagca gcgcaatatc cgctcggtcg gctgactatc cgagcggtgg gcgggaagta
      661 tcggattgca tccgagctaa agaaactggg ggccgaagcg ggccggtgtg cggcgctatt
      721 ggatcttgat gagcggagtg tgcctgatgc cgtggctcgc gctcgggatc agttgggaca
      781 accgacggtc gaggtctttt gtgccatccc agcgattgag gcttggttgt ttgcggatga
      841 caacttggct ctttctcagg cctctttgga tgaagagaca gcgtctgtgc tcagacggat
      901 tccgctgccc gaggaaatcc ccaatcccaa agagctagcc cgtgtgcttt ttggtccgcc
      961 agtgaactgg gcgttcatgc agaacatgga tgtgcagcgc gcttcggcac gatgcccatc
     1021 gctgcgtgca tttctgaccg gcatgggacg attgcttggc gtgtccacgc agccaatcga
     1081 agacagtgtt tcccgcagtt tgagtcgcaa tgtgtttgct ggcctccttg cggaaatcgt
     1141 tccttccgat gccattgcat ggcgaaccgc aagtggggaa acactgacgg ctgccgcgct
     1201 gcgcaaagag atcgaatcag gaacggaact ggggcatcag tatgcttccg acctcctgcg
     1261 tgtctcgcgt gactttttgc gacgaatcgc cagccgagct gagagccgaa ccgacccgag
     1321 catcaaagcc tccaaatgat tctgtttgct cccagctatg accccgcaac gggcgcaaac
     1381 ttggctgttg cccaggaagt cgccacgacc gcctgccaat gcttgttcca ggaagccgcc
     1441 actgcggaag cgctccacac tgcactcatc cagtcaccgc agccgctgtt tgcctggtca
     1501 cacggacgtc ccgcacagtt actcgcccag cacggccaca cagccatcag cgagccagtg
     1561 cttgcagtcc ttggtgcgcg acaggttttt gcgttcgcct gtcacaccgc cacgcggctg
     1621 ggacaactgg catcccagca aggcaccgtt tggtggggct acacgggggc cattcaatgc
     1681 ccggacgaca gtcccgcggt acttcccctg tttgtctcga ttttcaagtt cctgcgagaa
     1741 gaattttcca cagcggacac gacggacctt cgaagcgtcg tcatctcatc cctcgcgaag
     1801 atgtgcgaag cggcagcctt ttccatcgac gatctcgccg cttcaaatcc gacgatggtg
     1861 gtgcatgaag cactgctgtg tcttcatcac ctgtgggatc gcctgcgagt ctgggctccg
     1921 gatggagcat ttccagagca tcatcccgca gcgtccccac catccctctt tgctgattag
     1981 ccgcagtaca cttcgccgtt ctgatggggg ggtctggctc gccttcggct tggctctggc
     2041 tggggggaat tgggggggtt acttgacgta gacgcctagt tggtagtcgc ctttgccgga
     2101 gtttactttg gcttcgattt tgtacatgcc ggggacggtg gggcagtggc ccatcatggt
     2161 gcgattgctg ctggcgcggt tgtcgctgac gcgctgaagc tgggggttcc acaggtatag
     2221 gtagagttcc ttgacggtgg gggcgccggc gccgatgaac cagtagcagc ggcctggctc
     2281 tagggtggtg aaccagtcgc tgcggtcgtt tccaccgccg gctacgccgc gatagaagtc
     2341 gcctacgcgg acggcgcctg gggcggcggc ggcggcttgc tggtcgatga tggggcccag
     2401 gtcaagcggc ggcggggcca cgggcggggg gggtggcgcg gctttcggcg gcgcggcgac
     2461 ggcatagaca ccgacggcca tcggtccaaa gccttcgccg gtcttggctt gcaggtgata
     2521 gctgcccgac acgtctgggc agtagcgcag ggtgacttgg ggccgctcgg gcttttcggt
     2581 cgcgacgcgc ttttccgcct gatcccacag atacaggtag agctgcttta cgccctggcc
     2641 gccgatgccc gaaaacgtgt agcacaggcc ttcctgcagc ggcaggttcc agtccgtgcg
     2701 gccatcttct tcggtcgtgg ccgacagaaa gtgcggtccc acaagctgcc cggcgggctc
     2761 ggctttgagc gcttgggcat cgacctgcgg gatcagcgtc tcgctagacg gaaccggtcc
     2821 gctgtgcgtc ggcgtatgcg aacagcccag cgcaagcgca aacaggggca ggcccagtgt
     2881 ccccatagaa acgaaacgaa tcgacattac gtggttctcc ttctcggcac ttttccaaaa
     2941 acaaccgcgt cacaggacgg cggtttccat cgccaactga cctcgaagcg tacccaactt
     3001 tgcctgccgc tgctacggta cgcggcgaga aattgccacc caagccctca cccccccgcg
     3061 acgcggaggc gggtctgggc actgcgaaag cgggcggatg cctgcgcaac gcggtccggc
     3121 tcgatgctct tcaaaagcag cggcccatcg acttgcttgc tgtagcgaag gactgcggtt
     3181 tcgggctggt tccgtcgaat gtcagccagc ttgcaggggc acggatctat cgcggtgaag
     3241 ctcgcttgca gggcggagct gagcttgtgc tcacagctgc cgttttgcag tcgaccgcca
     3301 aactgatcga tttgggcaaa cgtgagtggg gccaaaatgc aggtctcgac gtgcttgcgc
     3361 agcgcgtggg caatcggacg gtagtgcttg gcgaagctgt ggttccagtc ggctagcagc
     3421 caggcttcga tttcaggcca tgccagcagc gcgtggaatg cgagggtggg cttgccggtg
     3481 gcggcgcgca cttgttcggt caggtcagct tcccacgcca ggaaggctgc ggtgacgtcg
     3541 ggggcgcccg caaagcgaca gtcagcatcg tcgataagaa ccacaaaatc aaaagagcag
     3601 gccgccccgt gatagtgctt ggccaaaagc tccagcatgg tgtcgacaag atactgaccg
     3661 cttgcgcccg actgcccgct gactggaacc ggatggggac ggtgtagctt cggcgccggc
     3721 ttgcgtaccg ccggaaagca gcgctcccac ttcaccttgg ggtcgagctt ttgcaaaaag
     3781 tcgtggatgc cgccggtttc ggtgtgcgcc gcagtgcaaa agtagccgcc gcttaccacg
     3841 gccagccccc gagcttcggc tcgccaaccc ggtacaagtc gcccagctcc cagccgtctt
     3901 tccagaagct ttccagctcg cccggttcga cgcggcgcat ttctttgtcg cccagaccaa
     3961 agacaaatag cgcgacctcg ccggtcttaa atccctcggt gaaggtgtcg agcaggtccg
     4021 gcgagtgggt gctgacaaag agctgctctg ccgacgaaaa ccgctggagc cagcgcccaa
     4081 tcaccttgag ccacgccggg tgcaggttca tttccggttc gtcaatcgac agcaccgatc
     4141 ccttttccgg tgaaaagagc aaaagcgcca gccacatcgc cttgagcgtc ccgtccgaca
     4201 tctcacgcgg tttgtacttg tgaccgccga tgttaagctc cagctgcttg taggtgtcgc
     4261 tggcgtcgat gaccttgagc ttgcgcaggt caggacaaag ctcgcgcagc cgctcggcgt
     4321 attcatccag aaagtcgtac ttctggtcga ggtagcgcag cacattgaca aactggctcc
     4381 cctcggcatc gagcgcccgc acgctcaggt caatcttggc cccttcggcg agctggcgaa
     4441 cgtccaggtc cgtgctcgca tacgaatgaa acccccgcgc aaacgccaaa agctcgtcct
     4501 gtgtcttcga aaattccggg tagacgtgct catagaactg cttgtctttg agcagcttcg
     4561 ccgactgttt caggatcgtc tcattggtgt cgacatcgac aactagcgtc ggcgtcagct
     4621 ttggcttcgg ttccttgcca tgtgtgacca agccaaactc acactgatcc gcccgtcgtc
     4681 caccacgtct cgcgagaagc ttgcctctcg ccgatgacca acgtcggtca ataatcggcg
     4741 cgtgggcaaa ctcgagcagt tcttcaatct cgacgtgaaa acccatcggg aaatcagcgc
     4801 tgtcgccaac cacataggaa agggcgtaca gcaagtcacg cggtgacggc tggaccttca
     4861 ggatcgattc gacctgcgag ggttgtcgca ccgattgaag cagccactga aaggccaagc
     4921 gacgagtgcg agttttagag ccatgcgtgc ggttgaccac ctcggccctg ccgtgctttt
     4981 cgacggtctg caaaaaagag gttg
//
LOCUS       DAORGN010000284         5000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8599_length_5000_cov_6.058241, whole genome shotgun sequence.
ACCESSION   DAORGN010000284 DAORGN010000000
VERSION     DAORGN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8599_length_5000_cov_6.058241"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..69
                     /locus_tag="PKL17_20285"
     tRNA            2..69
                     /locus_tag="PKL17_20285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30..32,aa:Arg,seq:ccg)
     gene            85..981
                     /locus_tag="PKL17_20290"
     CDS             85..981
                     /locus_tag="PKL17_20290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNK47133.1"
                     /translation="MNAASPSEHPSGVRSRRRSTVLLALSSFLFAMMAVAAKESSQRL
                     PGAEVACLRFVIGALTVGLFSLCGLRLRPHSLSVLLLRGLLGSGAVLLYFVAIAHLPV
                     GVATLLNNSSPLFVAAFSWLFLGEPLLARTLWAFLLTTAGVLMVVLGNSPLGGGRSLD
                     LRYMLLGLGSAVLAGGAVTTIRAMRRREGSWEIFFAFCVIGALVTAVPTAYQFVWPTR
                     RDAFWIAMTGLFSSAYQVMMTYSLKDVPAVQAGLLLQLTPIATFAFGALWLGELPSLL
                     GWLGAALTIFGVCWGLLVPAKK"
     gene            complement(1060..1503)
                     /gene="rplI"
                     /locus_tag="PKL17_20295"
     CDS             complement(1060..1503)
                     /gene="rplI"
                     /locus_tag="PKL17_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNK47134.1"
                     /translation="MQVILREDISNLGKSGELVSVKPGYGRNFLIPQGLAVLATERNV
                     KLMEHHKRTIAQRNAKLLKDAQSVSDKLAGITLKFARQAGDAGKLFGTVSTRDIEEEA
                     KKVGVSLDRKKIHLAEHIKTVGDYTVDIKLSQGVVAKLKIQVTAA"
     gene            complement(1516..1839)
                     /gene="rpsR"
                     /locus_tag="PKL17_20300"
     CDS             complement(1516..1839)
                     /gene="rpsR"
                     /locus_tag="PKL17_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNK47135.1"
                     /translation="MSMEMDENQGAPRRGPRGAGQGGADKKGGSSVRKRGFTRRKVCR
                     FCADKTLVIDYKDPQLLKYFVTDRGKIVPRRISGNCAKHQRKVALSVRRSRMIALLPY
                     TVTGA"
     gene            complement(1843..2367)
                     /gene="rpsF"
                     /locus_tag="PKL17_20305"
     CDS             complement(1843..2367)
                     /gene="rpsF"
                     /locus_tag="PKL17_20305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNK47136.1"
                     /translation="MAQTSMRDEPGTKREYETIFILRPEVNQDGISSVNNRVRNVIES
                     MGGKVLKLDNWGKRKLAYEIKKQLKGIYLYWQYLGSSGMVEEIERNLRMLESVVRYYT
                     VKVDEDVVADARPSAVDEDTYVKAATTIPDEEELATGAAVASRFREDDEDDDEFDPDL
                     EEAALSRHAEKGEE"
     gene            complement(2497..3102)
                     /gene="pth"
                     /locus_tag="PKL17_20310"
     CDS             complement(2497..3102)
                     /gene="pth"
                     /locus_tag="PKL17_20310"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016933065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNK47137.1"
                     /translation="MTWLVVGLGNPGPEYEQSRHNIGFMVADRLIEKWGGRKSDFRSK
                     WNSEVAQIEALGEKILIQKPMQYMNLSGGPVQRAAAFYQLQPSQIVVIHDDIDLDFGR
                     LKLKQGGGHGGHNGLRSISGLLGADYLRVRGGVGRPNVGGEAKGERVVGHVLGPFAKA
                     EQRELPAFLDTLADAVEDIVRHGLPYAMNRHNGDGRSKGSD"
     gene            complement(3102..3821)
                     /locus_tag="PKL17_20315"
     CDS             complement(3102..3821)
                     /locus_tag="PKL17_20315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNK47138.1"
                     /translation="MEVGKLTAQLRTTKGKETNTKLRQKGLIPAVCYGAGEAPLAIQL
                     DPTALQKSLDPVKGRNTLLMLTVEGKQGTIPVMVKDAQRHVLRGHFLHADLLRVDVSK
                     PVRATVPVSVTGKCPGVAAGGTLHQVYRTLPVSCVPGAIPSKIEIDVSGLNMGQAIHA
                     SDVKLPTGVSIALAANTTVCVVTAPKAEKGADAAAGAAAGAATDAKPGDKAASSKTPA
                     PAAAKAGGDKAAAKPAAGGKK"
     gene            4116..4649
                     /locus_tag="PKL17_20320"
     CDS             4116..4649
                     /locus_tag="PKL17_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47139.1"
                     /translation="MLTGLTVLFDCPPGLVSLVGDGDGNELVRLVPQGEVDACLLVSR
                     KPLFATGNQSLHPELLLDEVLAQQPQFRVVRRSPAEQIQSSVGKMHLLRVVGWHGEPK
                     IALGQLVALFSPSNPALPSCAAVSIFLWYTEKAGRKYAPQAMALFRSLRVMPVSQQSW
                     PPSDVLASVPPTSSVPT"
     gene            4766..>5000
                     /locus_tag="PKL17_20325"
     CDS             4766..>5000
                     /locus_tag="PKL17_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47140.1"
                     /translation="MNKKVLAAVVSTLAFGTASMASAGEEPAKAAKPAKEAKAKAPEK
                     KGDKAAEGDKGGAKSCGGDKGGAKSCGGDKGGAK"
BASE COUNT          832 a         1493 c         1579 g         1096 t
ORIGIN      
        1 tttagctcag ctggatagag cgttggcctc cgaagccaaa ggtcgcaggt tcgaatcctg
       61 caatgcccac cgaagcgggg gaacgtgaac gccgcctcac catccgagca tccctctggt
      121 gttcgcagtc ggcggcgaag cactgtgctg ctggcgcttt catcgtttct ttttgcgatg
      181 atggccgtcg ccgccaaaga gtcatcccag cgtctacccg gtgctgaagt tgcgtgcttg
      241 cggtttgtca tcggtgcgct gacggtgggg ctgttttcgc tgtgtgggct gcgcctgcgt
      301 ccgcacagct tatcggtgct gcttttgcgt gggcttctgg gcagcggagc ggtgctgctc
      361 tattttgtcg cgattgcgca tcttcccgtc ggcgttgcga cgcttttgaa caacagctca
      421 ccgctgttcg tcgcagcgtt tagctggctt tttctgggcg agccgctttt ggcgcggacg
      481 ctgtgggcgt ttttgctgac gacggctggc gtgctgatgg tcgtgctcgg aaacagtccg
      541 ctggggggcg ggcgctcact cgatcttcgc tatatgctgc tggggctcgg ctctgcggtg
      601 ctggcggggg gcgcggtgac gacgatccga gcgatgcgcc gacgcgaagg ttcgtgggag
      661 attttctttg ctttttgcgt cataggtgcg ctcgtgacgg cggtgccgac ggcgtatcag
      721 ttcgtttggc cgacgcgacg ggatgcgttt tggattgcga tgaccggcct attttcgagc
      781 gcctatcaag tgatgatgac gtattcgctc aaggatgtgc cggcggtgca agcggggctt
      841 ttgctccagc ttacgccgat cgcgacgttt gccttcggcg cgctatggct gggcgagctg
      901 ccatcgctgc ttggctggct tggggccgct ctgacgatat tcggtgtgtg ctggggactg
      961 cttgtgccgg cgaaaaagta acccgctccg cgaacatcgc cgagcgagag cgttacttgg
     1021 aaggcagagc agaagcgggc agggaagaag cgggaggtct tacgctgcgg tgacttggat
     1081 cttgagcttg gcgacgacgc cctggctcag cttgatatca accgtgtagt caccgacggt
     1141 cttgatgtgc tcggcgaggt ggatcttctt gcgatccagg ctgacgccga ctttcttggc
     1201 ctcttcctcg atgtcgcggg tcgagacggt gccgaacagc ttgccggcat cgcctgcttg
     1261 gcgcgcgaac ttgagggtga taccggcgag cttgtcggac accgactgag cgtccttgag
     1321 cagcttggca ttgcgctgcg cgatggtgcg cttgtggtgc tccatcagct tgacgttgcg
     1381 ctcggtggcc agaaccgcca gcccttgcgg gatgaggaag ttccgcccgt agcccggctt
     1441 caccgagacc agctcaccgc tcttgccgag gttcgagatg tcctcgcgaa gaatgacttg
     1501 catggtgatg actccttacg cgcccgtcac ggtgtagggc aaaagcgcga tcatccgcga
     1561 acgacggacc gaaagcgcaa ccttccgctg gtgcttggcg cagttccccg agatgcgacg
     1621 cggaacaatc ttgccgcggt cggtcacgaa gtacttcagc agttgcggat ctttgtaatc
     1681 aataaccagc gtcttgtcgg cgcagaaacg gcacaccttg cgacgggtga aaccacgctt
     1741 gcgaaccgac gatccgccct tcttgtcagc gccaccttgg ccggcgccac ggggtccacg
     1801 gcggggagcg ccctggtttt cgtccatctc catcgacatg gcttactcct cccccttctc
     1861 tgcgtgacgg gaaagcgcgg cctcttcgag gtcgggatcg aactcatcgt cgtcctcgtc
     1921 atcctcgcgg aagcgcgacg ccactgccgc accggtcgcc agctcttcct cgtcgggaat
     1981 cgtggtcgcg gccttgacgt aggtgtcttc atcgactgcg ctcgggcgag catcagcgac
     2041 gacatcctca tccaccttca cggtgtagta gcgaacgacc gactcgagca tacgcaggtt
     2101 gcgctcgatt tcttcgacca tgccgctcga gcccaggtac tgccagtaca ggtagatacc
     2161 tttgagctgt ttcttgatct cgtaagcgag cttccgcttg ccccagttgt cgagcttgag
     2221 cactttgccg cccatcgact cgatcacgtt gcgtacacgg ttgttgacgg agctgattcc
     2281 gtcttggttc acttccggcc gcaagatgaa gatcgtctca tactcgcgtt tcgtgccggg
     2341 ttcgtcccgc atggacgttt gggccatcac attcctcctg gctgataaag ccctcggacg
     2401 cgccgagggc gaggacaaaa cgggcaataa aaagcacaac tttctacgcc cggtcaagcg
     2461 caactttgca aaatcactcg gctttgcccg ctgggatcag tcggaccctt tgctgcgccc
     2521 gtcgccgttg tgccgattca tggcgtaggg cagcccgtgg cggacgatgt cttcgacggc
     2581 atcggcgagg gtatcgagaa acgcgggcag ctcccgctgt tcggccttgg caaatgggcc
     2641 gaggacgtgt ccgacgacgc gctcgccttt ggcttcgcca ccgacgttgg gcctaccgac
     2701 accgccacgg acccgcaaat aatcggcgcc aaggagccca gaaatcgacc gcagaccgtt
     2761 gtggccaccg tgaccgccgc cctgcttgag cttgagccgc ccaaagtcga ggtcgatgtc
     2821 atcgtggatc accacaatct gcgacggctg aagctgataa aaagccgctg cacgctgaac
     2881 cggccccccc gacaggttca tgtactgcat cggcttttgg atcaggattt tttcgccgag
     2941 tgcttcaatc tgcgcgactt cgctgttcca cttgctgcga aagtcgcttt tgcgaccgcc
     3001 ccatttttcg atgaggcgat cggcaaccat gaagccgatg ttgtgcctgg actgttcgta
     3061 ctcggggccc ggattgccaa ggcccacgac gagccaagtc actacttctt accgcctgcc
     3121 gctggcttgg ccgccgcctt gtcaccaccg gccttggctg ctgccggagc cggagtcttc
     3181 gacgaagccg ccttgtctcc cggcttggca tccgtcgccg cgcccgctgc tgcacctgct
     3241 gccgcatccg cacccttttc tgccttcggt gcagtgacga cgcagaccgt ggtgttggcc
     3301 gccagcgcga tgctcacgcc cgtcggcagc ttgacgtctg aagcgtggat cgcttggccc
     3361 atgttcaggc ccgacacgtc gatctcgatc ttcgagggaa tcgcaccggg aacgcagctc
     3421 accggcagcg tgcggtagac ctggtgcagc gttccaccag cggccacgcc tgggcacttg
     3481 cccgtcaccg aaaccggcac cgtggcgcgg accggcttgc tgacgtcaac gcgcagcagg
     3541 tcggcatgga ggaagtgacc gcgcagcaca tggcgctggg cgtctttcac catcaccgga
     3601 atcgtgccct gcttgccttc caccgtcagc atgagcagcg tgttgcggcc cttgaccgga
     3661 tcgagcgact tttgcagcgc cgtcgggtcc agctgaatcg ccagcggggc ttccccggcg
     3721 ccgtagcaga ccgccggaat cagacccttc tgacgcagtt tggtgttggt ttccttgcct
     3781 ttggtggtgc gcagttgcgc cgtgagcttg ccgacttcca ttgtgcgttt cctcttcggt
     3841 tgcttccgga atgtcggtca ctcgccgaac attcccgctc gctcggcccg cgccgaggag
     3901 ccgcctcgca gtgagcgcga gggggtctag taatgaaaag acaagaaaat cggccacaat
     3961 ttcgcgggct tgtcaagcgg gcgcttgggt tctctctcgg acgaagcggt ggtgagggtt
     4021 ctggcgtggt tcgccgtttg gcgcgagcgt tcgtgatacg atgtgtgctt ggccgcacga
     4081 ttttttcggc gcacaagata gggtctaaaa aagcgatgct gacggggctg acggtgttgt
     4141 ttgactgtcc tcctgggctg gtaagcctgg ttggcgatgg cgacggaaac gaactggtgc
     4201 gtctggttcc ccagggcgaa gtcgatgcct gtctgctggt cagtcggaag ccgctgtttg
     4261 cgaccggaaa tcagtcgcta cacccagagc ttttgctcga cgaagtgctg gcgcagcagc
     4321 cgcagtttcg cgtggttcgt cgatcacctg cggaacagat tcagtcgtcg gtgggcaaga
     4381 tgcacttgct tcgcgtcgtc ggctggcatg gcgaaccgaa aattgccctt gggcagcttg
     4441 tcgcgctgtt ttcgccaagc aatccagcgc tgccgtcgtg cgcagcggtg tcgattttcc
     4501 tttggtacac ggaaaaagca ggtcgcaagt acgcaccgca agcgatggcg ctgtttcgca
     4561 gcctgcgggt gatgccggtg tcgcagcaga gctggcctcc gtcggatgtg ctggcttcgg
     4621 ttccgcccac gagcagtgtt ccgacctgac agccgctgcg ttcctacaag aataatcggc
     4681 aatttgtgtg cagttgggga atctccgata tccttgtggt ttctcttcgg ccttgttggc
     4741 cacaaacgac caaaggagat cgctcatgaa caagaaagtt ctcgccgctg ttgtttcgac
     4801 gctcgcgttt gggaccgctt cgatggcctc ggctggtgaa gagccggcca aggctgccaa
     4861 gcccgccaag gaagccaaag ccaaggcgcc cgaaaagaag ggcgacaagg cagccgaagg
     4921 ggacaagggc ggcgcgaagt cctgtggcgg cgacaagggc ggagccaaga gctgcggcgg
     4981 cgacaagggc ggagccaaga
//
LOCUS       DAORGN010000285         4970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_8682_length_4970_cov_5.388810, whole genome shotgun sequence.
ACCESSION   DAORGN010000285 DAORGN010000000
VERSION     DAORGN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8682_length_4970_cov_5.388810"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1261
                     /locus_tag="PKL17_20330"
     CDS             464..1261
                     /locus_tag="PKL17_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47141.1"
                     /translation="MMRWFRGGVRWALCTLPFLSNGCAVPLQRSCPRCQALELGRRPA
                     VPFGTRTVFLLVPGLLGYGWEWDAAQAELAKIPFAVTLVWPWDPWKSLSQSGDALARH
                     VGYLVRRVPDSVDRVVVVGHSAAGLLVLWAASRVFVPSRLSLTMVSVGAPLAGQGFNP
                     WGIPDELRTPLPIALGATFSPWPEPADGVKLRVFVTGPSDPVMAWHFGHNPGSRVVLP
                     PSAEVVALPNSLDHNFALGEIAKELRSVELSARQAVPGKPVWPHAAP"
     gene            complement(1284..2492)
                     /locus_tag="PKL17_20335"
     CDS             complement(1284..2492)
                     /locus_tag="PKL17_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47142.1"
                     /translation="MRSALSVLKRGSLRQLPSWLAVAAIVFCLGFRHTAHAGADAGDA
                     AARLDDRPAASAEEEQTARALFDGAMQRISGASQATAVAELVALADRFPKTTLAPEAL
                     FAAAQQKDESLGAPDQALVLYQRVVVEYPDSRLIRRAQARVAELSASLGSGAAVLTQF
                     QHIVRSTTSESPERSARLDQLLREQPTFVLAPEALYLLLDGAIRRGDAQAPQRLKQLA
                     DRFPASEWTARGKKAYGDQLLQKGQLGQARAMFLSLSDHPAPLWQKAAAEGISAVSQA
                     ETRRLLATLCGLTWAASLLWLAVRHRQRLWPPPVEVLYYVPVGLFFVLTAALLQNGVF
                     VRPLLVLVVGGGVLCWLSAAAARAGRARRLAFGLAWRAVLAALLCYLAIERQGLWELV
                     LETLRNGPEG"
     gene            complement(2510..4201)
                     /locus_tag="PKL17_20340"
     CDS             complement(2510..4201)
                     /locus_tag="PKL17_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNK47143.1"
                     /translation="MQRAQVRTTRPAWRTWAQLVCCLLCFGFAIGCGPSESEPQPPPQ
                     AQLLLLSPKSPLGLSYGEQTTLQFRYLVQGEPQAGVALKLWIDSTTGGGTLSTPTAVT
                     NDLGEASVRLTAGTTESAFHVTVSAPQTTDLVVDVAVSRFAFGTLRVLLDASPVSLAA
                     VQLRVGLYPNTACSDLSPTPVLMGALRSQRQNGPRGELLFGTLLIQPYAVVGRAEDQS
                     GRLIGYGCVEVPERLLRADLNIPVEVPLGIVQPSPVGSYLLDIQITPQWPAGQPWLSL
                     TCTRGLGLTFVDALLEALGPSDLAMRLLAARGGQDAKGCRLDSAQPGDPDRLLDGLLQ
                     ATTSGPQLGSVASEFAPALREQTAKSRLTVRGSTALGFVGEHALTSIDLKTLTMTQTY
                     SLATAPRPVASELVLTVRGPQLMVQSHELTIRLPRLWQKALSDLVLAPKGVTMTPGQL
                     LQAAVTAARYSTQSGCQAIESAICDLHASPCKGLVLPACQTAMLSVSAQLQGIVTDPK
                     AAADLLLGLTFQMQDPDGTLFAQKLHLGQLAGEAALGVGSVKLSGTATGVRDPKP"
     gene            4276..>4970
                     /locus_tag="PKL17_20345"
     CDS             4276..>4970
                     /locus_tag="PKL17_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNK47144.1"
                     /translation="MATPLASDSALRSPFRSDLLTGQVAVVTGGGTGIGRAIALELAG
                     CGAAIAVCGRRPDPIAAVKADIETAGGTCFAMPCDIRDAQQIAAFVSATKAALGSIDI
                     LVNNAGGQRAFLAADMPLTNFEKVVQNNLVGTFAMTQAVAQQAMIPQKRGSIVNIIAM
                     ILRGFPGMVHTGAARAGVDNMTKSLAVEWSPHRIRVNAVAPGIIRTSGTDRYPPELLE
                     VARRATPWKRLGLA"
BASE COUNT          906 a         1548 c         1584 g          932 t
ORIGIN      
        1 ggcccgccat ggaaaaccaa tctcaaccac acctacgctg gatcgcgaag acccacatgt
       61 ggaaataatg tgtgatctat gtagatatcg gaacgaattc ctaagtcatg tcgcctggtt
      121 ggatgaagtg gtcgaaattt atttgtggat aatttgcttc cacttatcaa aaaaaccttg
      181 ccaaactgca aaatcgtgct tgtcgaaaat ttcataataa atccggatgc ttggcgggtg
      241 tgtcgatagc tgctcatttg taggattcgt cggtggatgt tgtgaagatg gctgccagat
      301 tggctgattc gggtcttccg atttttgtac caattggtac gcctgtgaac acgggtttgg
      361 tatctccgag gccgagctgt gtggatgtct cagtggccgt accggcaaga acaaccgaaa
      421 gagtgctgga tcacggtgcc gacggagcta ggatgatctc gcgatgatgc gctggtttcg
      481 gggcggcgtg cgatgggctc tctgcacgct gccttttttg tcaaatggct gtgcggtgcc
      541 gcttcagcgt tcctgtccgc gctgccaggc gctggaattg ggcaggcgac ctgccgttcc
      601 gtttgggacg cggacggtct ttctgcttgt gccggggctt ttgggctatg gctgggaatg
      661 ggacgcagcg caggccgagc tggcgaagat cccctttgcg gtgacgctgg tgtggccgtg
      721 ggacccttgg aagagtcttt cgcagagcgg cgatgcgctg gcccggcacg ttggctactt
      781 ggtccggcgg gttccggatt cggtggatcg cgtggtggtc gtcggacact cagcggcggg
      841 gctcttggtt ttgtgggcgg cgtcgcgggt ttttgtgcca agccggctct cgctgacgat
      901 ggtgtcggtt ggtgcgccgc tcgcggggca gggttttaac ccgtggggca ttccggacga
      961 gctgcggacg ccgttgccga ttgcgctcgg ggcgacgttt tcgccgtggc ccgagccagc
     1021 ggacggagtg aagctgcggg tgtttgtgac cggacccagc gatccggtga tggcttggca
     1081 ctttggccac aatcccggca gtcgggtggt gcttcctccg tcggcagaag tcgttgcgct
     1141 gccaaactcg ctggatcaca actttgcgct gggcgagatt gccaaagagc tgcggagtgt
     1201 cgagctttcg gcgcgacaag ctgtgccagg aaagccggtc tggccgcacg ctgcaccgtg
     1261 acgaaaaaag cgcgtggctt gaatcagcct tcgggaccgt tgcgcagcgt ttccaagacc
     1321 agttcccaaa gcccttggcg ctcgatcgcc aaatagcaca gcaaggccgc cagcaccgca
     1381 cgccaagcca gaccaaacgc cagccgccgc gcccgacctg cccgcgccgc cgctgctgac
     1441 agccaacaca gcacaccgcc tcccacgacc agcaccaaaa gcggacgcac aaacaccccg
     1501 ttttgcaaaa gcgccgcggt cagcacaaaa aacagcccaa ccggaacgta gtaaaggact
     1561 tcgaccggcg ggggccaaag gcgctgcctg tgcctgacag cgagccacag aagcgaagcg
     1621 gcccatgtca gaccgcagag cgtagccaag aggcggcggg tttcggcttg cgagaccgca
     1681 gaaatccctt cggcggcggc cttttgccaa agcggcgcgg gatgatcgga aagcgacaga
     1741 aacatcgctc gcgcctggcc gagctgacct ttttgcagaa gctggtcgcc atacgctttt
     1801 tttccgcgtg ccgtccattc gctggccggg aatcggtccg caagctgctt cagccgctgc
     1861 ggtgcctgtg cgtcgccgcg ccttatcgcc ccatcgagca gcaggtacag cgcttctggg
     1921 gccagcacaa acgtcggttg ctcgcgcagc agttggtcga gccgtgcgct gcgttccggg
     1981 ctttcgctgg tcgtcgagcg cacgatgtgt tggaactggg tcagcaccgc ggcccccgac
     2041 cccaggctgg ctgaaagctc ggcgaccctg gcctgcgcgc gacggatcag gcgactgtcc
     2101 ggatactcga cgacgactcg ctgatacagc acaagcgctt ggtcgggcgc gcccagcgat
     2161 tcgtctttct gctgcgccgc ggcaaacagc gcttccggcg ccagcgtcgt tttgggaaag
     2221 cggtcagcca gcgccacaag ctctgccacc gccgtcgctt gcgaagctcc gctgattcgt
     2281 tgcattgcgc catcgaacag cgcgcgcgcg gtctgttctt cttcggcgct ggccgctggc
     2341 cgatcatcga gccgagccgc cgcgtcgcca gcgtctgccc cggcgtgtgc ggtgtgccga
     2401 aagccaaggc aaaacacgat cgctgcgacg gcgagccaag atggaagctg gcgcagcgaa
     2461 ccccgtttca aaacagacag tgcagagcgc atcggttggt cggctcgatt tacggcttgg
     2521 gatcacgaac gccggtcgcg gtgccgctga gcttcaccga tccgactccg agcgcggcct
     2581 cgccagcaag ctgccccaga tggagcttct gcgcgaacag cgttccatcg ggatcttgca
     2641 tctggaatgt caggcccagc agcagatccg ccgccgcttt gggatcggtg acaatgcctt
     2701 ggagctgcgc cgagacgctg agcatcgccg tctgacaggc cggtagcacg agccccttgc
     2761 agggacttgc gtgcaggtca cagatggcgc tttcgatcgc ctgacagccg ctctgggtgg
     2821 aatagcgcgc cgccgtcacc gctgcttgca agagctgtcc cggcgtcatc gtcactcctt
     2881 tgggcgccag caccaggtca ctgagcgctt tttgccaaag ccgcggcagg cgaatcgtca
     2941 gctcgtggga ctgcaccatc agctggggac cgcgcaccgt caggacgagc tcgcttgcca
     3001 ccggacgcgg cgccgtcgcc agcgaatacg tctgcgtcat ggtcagcgtc ttcagatcga
     3061 tcgaagtcag cgcgtgttcg ccgacaaaac caagcgccgt cgagcctcgc acggtcagtc
     3121 gcgacttcgc ggtctgttcg cgcagtgctg gcgcaaactc gctcgcgacg ctcccgagct
     3181 gcggaccgga agtcgttgcc tgcagcaatc cgtcgagcaa acggtctgga tcgccaggct
     3241 gcgcgctgtc cagccgacac cctttggcat cctgaccgcc ccgcgccgca agcagccgca
     3301 tcgccagatc acttgggccg agcgcttcca aaagtgcgtc gacaaacgtc aggcccagcc
     3361 cgcgtgtaca ggtcaaagac agccacggct gcccggcggg ccactgcggc gtgatctgga
     3421 tgtcgagcag gtagcttccc acgggacttg gctgcacgat acccagcggg acttcgacgg
     3481 ggatgttgag atcggcgcgc agcaggcgtt cggggacttc gacgcagcca tagccgataa
     3541 ggcgtccgct ctggtcttcg gctcgaccga cgacggcgta gggctgaatc agcaacgtgc
     3601 caaacagaag ctcgccacgc ggaccgtttt ggcgttggct gcgcagcgcg cccatcagca
     3661 cgggcgttgg tgacagatcg ctgcacgcgg tatttggata cagtcctacc cgaagctgta
     3721 ccgcggccag cgacacaggc gatgcatcga gcaggacgcg gagcgtgcca aaggcaaaac
     3781 gcgacaccgc tacatccacc accaagtcag tcgtctgcgg cgccgagacc gtgacgtgaa
     3841 acgccgattc ggtcgtgcct gcggtcagtc gcaccgacgc ttcgccgagg tcattggtga
     3901 ctgcggtggg cgttgaaagc gtaccgccac ccgtcgtcga atcgatccac agcttcagcg
     3961 cgacaccggc ctgcggttcg ccctggacca aatagcgaaa ctggagcgtc gtctgttcgc
     4021 catacgaaag cccgagcggc gacttgggcg aaagcagcag cagctgcgcc tgcggcggcg
     4081 gctgcggttc actttcggac ggaccacatc cgatcgcaaa accgaaacag agcagacagc
     4141 acacaagctg cgcccaggtt cgccaggccg ggcgggttgt tctgacttgc gcgcgctgca
     4201 tccgtccctg catacacttc accctaatcc gcttgcctgg ccactggcta gaaaagccct
     4261 gctagagtgc tgcgcatggc aactccgctc gcttctgact ctgcactccg cagtccgttt
     4321 cgttccgatc ttctgaccgg ccaagtcgcc gtggtgacag gtggtggcac gggcatcggt
     4381 cgcgccatcg cgctggagct tgccggctgc ggggctgcca tcgccgtctg tggtcgccgt
     4441 ccggacccga ttgctgcggt caaagcggac attgaaaccg ccggcggaac ttgttttgcg
     4501 atgccgtgtg acattcgcga tgcccagcag attgccgcgt ttgtttccgc gaccaaagcc
     4561 gcgctcggat cgatcgacat cctggtcaac aacgccggcg gacagcgtgc ttttttggcc
     4621 gccgacatgc cgcttaccaa ctttgaaaaa gtggtgcaaa acaacctggt cgggacgttc
     4681 gcgatgacgc aggcggtggc gcagcaagcg atgattccgc aaaagcgcgg cagcatcgtc
     4741 aacatcatcg cgatgatcct ccgcggcttt cctggcatgg tgcacaccgg cgcagcaagg
     4801 gcaggcgtcg acaacatgac caagagcctg gcggtggagt ggtcgccgca tcggattcgc
     4861 gtcaacgccg tcgcgcccgg catcatccgc acgagcggca ccgatcgcta tccgccggaa
     4921 cttttggaag tggcgcggcg ggcgacgccc tggaaacggc tgggtcttgc
//
LOCUS       DAORGN010000286         4804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9157_length_4804_cov_5.278164, whole genome shotgun sequence.
ACCESSION   DAORGN010000286 DAORGN010000000
VERSION     DAORGN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9157_length_4804_cov_5.278164"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="eat"
                     /locus_tag="PKL17_20350"
     CDS             complement(<1..1314)
                     /gene="eat"
                     /locus_tag="PKL17_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HNK47145.1"
                     /translation="MSTNALPATEPPALKKFLRPIHLWAIAVGLVISGDYFGWNFGLK
                     ETSPFGMLLAVLCVSVMYICFIFSYTELSTAIPHSGGPYAFARRALGPLGGLVAGVAT
                     LLEFVFAPPAIALAIGSYVHFRMPQLGKIQVAVLMYFVFAALNCWGVELAVTFELFVT
                     GLAVFELLLFFGICGPHVKLANLLSEPLLPTGIKGIFAALPFAIWFYLALEGVAMSAE
                     EVVRPERDIPRGYIAGLCTLVVLALGTLICTSGVTQTANLIQDESPLPHAMATVLRPD
                     HPLTHMMVYIGLFGLLASFHGIMMGYSRQVYALSRGGYLPPFLSFLHPKRKTPVFAVI
                     IPAIVGLLVVWLDKTEQAITLSSIGATVLYVVSMISLLVLRKREPDLRRPFLAPLFPF
                     FPAIALSLALLCLFAVVSSQPWLALLALGFFVIAVLYYLGFAKDRV"
     gene            complement(1448..4591)
                     /locus_tag="PKL17_20355"
     CDS             complement(1448..4591)
                     /locus_tag="PKL17_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNK47146.1"
                     /translation="MSASGVSTWVHGAVRKRNLVLFLTLLLVLASSVMALRLKLDALP
                     DITNNQVLVLTRAPGFSPEEVERLVTRPLEAALGGMQQLHELRSISRYGISAVTAVFD
                     DDAPPLAARQITQERLTTVSLPPGVDTPSLGPLTGGLGEVFHFTLSSQKRTQAELLEL
                     ATLRIAPRLKMVSGIVEVNTWGGAERRIEIQADPLRLSARGLTLDTLAAQLQKSVGAT
                     AGASIPSGDGQALLRAVYRPSRPEQLSPLLIPGPADSSGRPQRLIIGELGRVTEGLSP
                     RLGAATQDGQGEVVYLMAQMLRGENALDVLSGVTQRMEEIRKSLPPDVELHVVYDRSE
                     LVHATLRTVGKNLLEGGILVALVLLLTLGSWRAGLLVALVIPLSMLGAAVAMVLLGVP
                     GNLMSLGAIDFGLLVDGAVVMVEHLFHHPSAQHAGDEAETPRDRFIRSIGHRAAEVAQ
                     PVLFSVLIILLVYVPVVLLGGVEGKMFRPMALTVVCALFASLLLALLFVPAASAWLLR
                     PQDLIEERPPLLIRLMHRGYRPLLARSLRHPLGVGLLALLLLGSGVTLLLRSGSEFIP
                     QLDEGDLVIQTTRGADISLEQAVRKAQNMEQALREIPEVTRAVSRIGSPAVATDIMGI
                     EQADVFVGLKPRSEWRPHLAREQLIQEMETVLQRRDPGSEPSFTQPIQMRFNEILAGA
                     VTDVVVSVYGDDLAEIERLAHQVAKSCEKEPGAEDVRVLAPPAVPLVEVRPRAIEANQ
                     AGLLPSEVLSAVQAIRSGLPIGTTYEGPVRLPIVLRLGDSLPEAATLDAFPLPLPDGG
                     TAPLGRVADVSFFPTPTLIQRQNGERRLMVGFNVRGVDLGTLLSHAEQRVKREVPLPP
                     GYRLEWGGQYETFEAARARLGILIPVVLIIILGVLWRLFGSLRPTLILFSAVPFAGTG
                     GMIALALRGMPVSISAAIGFIALSGIAVLNGVVLMVRLLDLERQGHTPAQAAEEAAKT
                     RMRPVLVTALVAALGFVPMAMAEGVGAEVQRPLATVVVGGLITSTMLTLLILPTLYPA
                     LSRRRHPTADIAKPKTA"
     gene            complement(4588..>4804)
                     /locus_tag="PKL17_20360"
     CDS             complement(4588..>4804)
                     /locus_tag="PKL17_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47147.1"
                     /translation="LLIPSRALQSFARKSTVRRRRDGKMDTIAVDVLLQAAGLAVVKP
                     SDPVALTAQDTVAVSEQSASLGQGTSP"
BASE COUNT         1067 a         1516 c         1399 g          822 t
ORIGIN      
        1 gacgcggtct ttggcaaacc ccaaataata caggaccgcg atcacgaaaa agccgagagc
       61 aagcaacgcg agccacggct ggctggatac caccgcgaac agacagagca gcgccagcga
      121 cagtgcaatc gccggaaaga acggaaacaa gggggccaaa aaggggcggc gaagatcggg
      181 ttctcgcttg cgcaagacca aaagtgagat catcgacacc acgtacagca cggtggcgcc
      241 aatcgatgac agcgtgatgg cttgctctgt tttgtcgagc caaaccacaa gcagtccgac
      301 aatggcggga atgatcactg caaagaccgg cgtcttccgt ttcggatgca gaaacgacag
      361 aaacggtggc aaatacccgc cgcgtgacag cgcatagacc tgccgcgagt agcccatcat
      421 gatgccgtga aacgaagcga gcagaccaaa cagaccaatg taaaccatca tgtgggtgag
      481 cggatgatca gggcgaagca ccgtcgccat tgcatgcgga agcggcgatt catcttggat
      541 caaattcgcg gtctgtgtga caccacttgt gcagatcagc gtacccagtg ccagcaccac
      601 cagcgtgcac agaccagcga tgtagccgcg cggaatgtct cgttccggac gcaccacttc
      661 ttcggcggac atcgcgactc cttccagggc cagataaaac caaatcgcaa agggaagggc
      721 ggcaaagatt cctttgattc ccgtcgggag aagcggttcc gaaagcagat tggctagctt
      781 gacgtgtgga ccacagatgc cgaaaaacaa cagcagctcg aacactgcga gcccggtcac
      841 aaacagctcg aacgtaaccg ccagctcgac gccccagcag ttgagtgcag cgaacacaaa
      901 gtacatcagc accgcgactt gaatcttgcc cagctggggc atgcggaagt ggacatagga
      961 accaatcgcg agcgcaatcg ccggcggcgc gaacacaaat tccagcagcg tcgcaacccc
     1021 cgcgacaagt ccacccagcg ggccgagcgc gcggcgggca aatgcatacg gaccgccaga
     1081 gtgcggaatc gccgtcgaca gctcggtgta actgaagatg aaacagatgt acatcacaga
     1141 cacgcagagc accgcaagca gcatcccaaa cggactggtt tctttgagtc caaagttcca
     1201 gccaaagtag tcgccagaga tcaccagccc aacggcaatc gcccacaggt gaatcgggcg
     1261 aaggaacttt tttagcgctg gcggttccgt cgctggaagc gcgttcgtgc tcatggttgg
     1321 tccttttttt ctccatgggc aggctagtcg cagcgcggcg caagttgcaa aaaatcgcgc
     1381 cgaaaattcc accgcaccag ggattctgtt cccgcggcgg acgttcggat cattctgctt
     1441 ccgcttgtca cgcggtcttg ggctttgcga tatctgctgt gggatgtcgg cgccgactga
     1501 gcgctggata cagcgttggt agaatcagca gcgtcagcat ggtggacgtg ataagtccgc
     1561 ccaccaccac ggttgcaagg ggtcgctgta cttcggcacc gactccttct gccatcgcca
     1621 tcggaacaaa gccaagcgct gcgacaagcg ctgtcaccag caccgggcgc atgcgggttt
     1681 tcgcggcttc ctctgcggct tgcgcgggcg tgtggccttg ccgttccaga tcgagcaaac
     1741 ggaccatcag caccacgccg tttaacaccg cgattccgga cagcgcgata aaaccaatcg
     1801 ccgccgagat cgagaccggc atgccgcgca gcgccagcgc aatcattccg cccgttccgg
     1861 caaaaggaac cgcagaaaac agaatcagcg tcgggcgcag actgccaaac agccgccaca
     1921 ggactcccaa gatgatgatc agaaccactg gaatcaggat tccgagccgc gcccgcgctg
     1981 cttcaaatgt ttcgtactgt ccaccccatt ccagccgata tccgggcggc agcgggactt
     2041 cgcgtttgac tctctgttcc gcatgcgaaa gcaaggttcc cagatcgaca ccgcggacgt
     2101 tgaagccgac catcaagcga cgctctccgt tttgcctttg aatcagggtc ggcgttggaa
     2161 aaaacgatac atcagcgact cgacccagcg gtgccgttcc gccatctgga agtggcaatg
     2221 ggaatgcatc cagggtcgcc gcttccggaa gggaatcacc gagtcgcaag acaatgggaa
     2281 gccttaccgg accttcatac gtcgtgccaa tgggaagacc gctgcggatt gcttgtaccg
     2341 cagacagaac ttcgcttggc agcagaccgg cttggttggc ttcgatcgcg cgcgggcgga
     2401 cttcgacaag cggcacggca ggtggggcca gcacgcggac atcttcggcg ccaggttcct
     2461 tttcacatga cttggcgacc tggtgagcca gtcgctcgat ctcggccaga tcgtctccgt
     2521 acacgctgac cactacgtcg gtgacagcac cagccaggat ttcgttgaag cgcatttgaa
     2581 tcggctgggt aaacgaaggc tcgctgcccg gatcgcgtct ctgcagcacg gtctccattt
     2641 cctgaatcag ctgttcccgc gccaggtgag gacgccactc gctgcgtggc ttcagcccga
     2701 caaacacgtc ggcttgttcg attcccatga tgtccgtggc gacggcagga ctgccaattc
     2761 gggacaccgc gcgggtcact tccggaatct cacgcagggc ttgttccatg ttctgcgctt
     2821 tgcgtaccgc ttgttccagc gaaatgtctg ccccgcgggt tgtctggatc accaggtctc
     2881 cttcatcaag ctgggggata aactcgctgc cactacgcaa aagcagcgtc acacccgatc
     2941 ccaacagcaa aagcgcaagc agaccgactc ccagcggatg ccggagcgag cgtgccagca
     3001 gcggacggta gccacggtgc atcaggcgaa tcaaaagagg aggccgttcc tcgatgagat
     3061 cctgcggtcg caaaagccat gcggacgcgg cgggaacaaa cagcagggcc agcagaagcg
     3121 atgcaaacag cgcgcacacc accgtcagcg ccatcgggcg gaacatcttt ccttccacac
     3181 cgccaagcag cacgacaggg acatagacca aaaggatgat cagcaccgaa aacagcaccg
     3241 gctgggcgac ttccgcggcg cggtgtccga tcgatcgaat gaagcggtcg cgcggggttt
     3301 ccgcttcgtc acctgcgtgc tgggccgatg gatggtgaaa cagatgttcc accatgacca
     3361 cggcaccatc gacgagcagc ccgaagtcaa tcgcacccag gctcatcaag ttgcccggca
     3421 cgcccagaag caccatcgcc accgcggccc ccagcatcga aagcggaatc accagtgcga
     3481 ccagaagacc ggcgcgccag cttcccagcg tcaaaagcag caccagtgca accaagatgc
     3541 cgccttccag caggtttttc ccgacggtgc gcagcgttgc gtggaccagc tcgctgcgat
     3601 cgtagacaac gtgcagctcc acatcggggg gcaggctctt gcggatttct tccatccgct
     3661 gcgtgactcc cgacagcaca tcgagcgcat tttcgccgcg cagcatctgc gccatcagat
     3721 agaccacttc gccttggcca tcttgggttg cggcgccgag tcgtggcgac agaccttcgg
     3781 tgacgcgacc cagctctccg atgatcagcc gctgtggccg acccgagctg tccgcggggc
     3841 cggggatgag cagcgggctc agttgttcgg ggcggctggg ccggtagaca gcgcgcagca
     3901 gcgcttggcc atcgccggac ggaatgctgg cacctgccgt cgcgccgaca cttttttgta
     3961 gctgcgcggc cagggtgtcc agcgtcagac cgcgcgccga aagccgcagc ggatcggcct
     4021 gaatttcgat ccgccgttcg gcaccgcccc aggtgttcac ttcgacgatg ccggacacca
     4081 ttttcagccg cggcgcaatc cgcagcgtcg ccagttccag cagctccgct tgggtccgtt
     4141 tttgcgacga cagcgtaaag tgaaacactt cgccaagtcc gccggtcaaa ggtccaagcg
     4201 atggcgtgtc cacccccggc ggcagcgaca ccgtcgtcag tcgttcctgc gtgatctgac
     4261 gggcggccaa aggtggcgca tcatcatcaa agaccgccgt caccgccgaa attccgtacc
     4321 gtgaaatgct gcgcagctca tgaagctgct gcattccgcc gagcgcggct tcgagcggac
     4381 gagtgaccaa gcgctcgact tcttcggggg aaaagcccgg cgcccgcgtc agaaccagca
     4441 cttggttgtt ggtgatatcc ggcagcgcat ccagcttgag ccgcagcgcc atcaccgacg
     4501 atgccagcac caaaagaagc gtcagaaaca gcaccaggtt tcgcttgcgc accgcgccat
     4561 ggacccaggt ggaaacgccg cttgcgctca tggcgacgtt ccttggccca gactggcgct
     4621 ctgttcactg accgcgaccg tgtcttgcgc agtcagcgcg acaggatcgc ttggctttac
     4681 caccgccaga cccgccgctt gcaacagcac gtccaccgcg atggtgtcca tctttccgtc
     4741 ccgtcgcctt cgcacggtgg attttcgcgc gaagctttgc agcgcgcgcg acggaatcaa
     4801 cagc
//
LOCUS       DAORGN010000287         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9201_length_4792_cov_6.192949, whole genome shotgun sequence.
ACCESSION   DAORGN010000287 DAORGN010000000
VERSION     DAORGN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_4792_cov_6.192949"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..967)
                     /locus_tag="PKL17_20365"
     CDS             complement(5..967)
                     /locus_tag="PKL17_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47148.1"
                     /translation="MFVGLTQNDFACYEPRKWKSNVYNRERLEVRQKLLALGQHCAGQ
                     LVGPDGAPLFLEASVEHPAVWNHKQVENQSIYFSRNEAARKQLDGFIDRQKPISSLLD
                     DPTPQRNHLFLGVTVQHESLDVVLKIHPDATVDRQNLERKLDEHYEAERLLGLLHELP
                     EGFTIGVLPTLHAVSADFSMEQLAEIIGSMPQSGGLGIPGQPVHYFAIGTSLSQTQFV
                     AMSAEEQSDWVSSQLAHLLPIYRFIVWTRENDFVSMAKVLDKEKTVRRQRGLQKGDKV
                     RIVRGMLAGKDGAVAEADAKGQLKVIVGKMVIKIDASDVERANA"
     gene            complement(1057..1488)
                     /locus_tag="PKL17_20370"
     CDS             complement(1057..1488)
                     /locus_tag="PKL17_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47149.1"
                     /translation="MRLLPCFRVHSADRFGLAIAVLVAGCMLSFGCAHRPSIAPDVAE
                     ARSEITRLESNIASRRSQIPIVASGSLSAHAVPAMSPSAAQSAPAGRCDGVCVAAQSI
                     CGYSRRICTLSEKIADEPSQRSCKNAERECSDASSQCASCR"
     gene            complement(1493..2041)
                     /locus_tag="PKL17_20375"
     CDS             complement(1493..2041)
                     /locus_tag="PKL17_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47150.1"
                     /translation="MTPSSWQSALLAMLLLLSTCSRDDLVGRSDEAMLLMLSEARALH
                     RRADIHLLDGEVPAAIADIREVLAIRFPTGNTDGEDTLLDAHARLARLHLSQGGEEAE
                     RQALAQVEAGRKLATRDSFFRANLESVAAEIYETRAKRQSDPGAQKEARKEAVRALER
                     AIAIDRHLLRTLLSQPEFTEEH"
     gene            complement(2038..2670)
                     /locus_tag="PKL17_20380"
     CDS             complement(2038..2670)
                     /locus_tag="PKL17_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47151.1"
                     /translation="MQDDREGQVDHRTHSDDPDAPPRSDELRAAKSLREALRETEDKH
                     DSDPLHPLSPRSAEARWLAAHLRFPTEMESLGEVRARGLARSARELVAAKRSQAELRS
                     STMQKLSRTLTGTGGLLLGAAVLLMVSWAILDQGSKLRSPPSMTMNQTTWLLRESLRR
                     GESPTARLDLLLQERLRHRRILSLRSGVTLVNRHHRVVSPNAAKEGDKVQ"
     gene            complement(2660..3277)
                     /locus_tag="PKL17_20385"
     CDS             complement(2660..3277)
                     /locus_tag="PKL17_20385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNK47152.1"
                     /translation="MQNPDRTGPDDSTERSALLAREKRAVEKAQSGDLAALEPILAVH
                     AEALFMGILGRVGERAQAEDILKDTFVTALERISTYTFQQRSIFHWLRQIAFHKVIDF
                     HRAQGRRKRLCQALKAELSESEDASAPLLLSAQEERTIAQQRIEATLQVLHERYATAI
                     RLRLCEEKSRATCAAALGVSVETFDVLLLRAVRSFRKAYGEPDAG"
     gene            complement(3385..4398)
                     /locus_tag="PKL17_20390"
     CDS             complement(3385..4398)
                     /locus_tag="PKL17_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47153.1"
                     /translation="MSDTSAKEQTGTEGGAGEAPSGAQPSSGPALPGLRGPDGGAVPP
                     MQGNGSGSISAMRPAGSMPGLPPPKGKGEIDDEHERWLITKEDRMDYGPFSLRDVKAQ
                     IERGSISAEHTITDTETNDKRRVADHPLLGQMAREWTAKHMELDRQMKEEAERAKYNR
                     AVVKMLAGIFAAVVVAGSGVGVWLVTRPKPQVVKQTGPQFTDDPLKGLQIAMQPMPPA
                     PPKKKKKAGPKNGAFDDSQSVDFNDGGDTLSAEDIQKTMMSNFSVLSGCLREEAARSP
                     QTKKIDLEFIVKGTGSVSSVRVNGSTSTPVASCAFAKMQSIRFPECKTCQKTVAAFSL
                     TLK"
BASE COUNT          835 a         1484 c         1438 g         1035 t
ORIGIN      
        1 tgtgtcatgc attcgctcgt tccacgtcgc tggcgtcgat cttgatcacc atcttaccga
       61 cgatgacctt cagctggccc ttggcatccg cttctgccac agcgccatct ttccctgcca
      121 gcataccgcg aacgatgcgg accttgtcgc ccttctgtag accacgctgc cgacgcacgg
      181 tcttttcttt gtcgagcacc ttggccatcg agacaaagtc gttttcgcgc gtccacacga
      241 tgaatcgata gatcggcaaa agatgggcaa gctggctgct cacccagtcc gactgttctt
      301 cggcggacat cgcgacaaac tgggtctgcg aaaggcttgt gccgatcgcg aaatagtgga
      361 ccggctgtcc ggggatgccg agtccaccgc tctgcggcat cgatccgatg atttccgcga
      421 gctgctccat cgaaaagtct gcgcttactg cgtgcagcgt cggcaaaacc ccgatcgtga
      481 agccttccgg cagctcgtgt aggagcccaa gcagtcgttc cgcttcgtaa tgctcgtcga
      541 gcttccgttc caggttttgc cgatcgaccg tcgcatcggg atggattttg agcacgacat
      601 ccagcgattc atgctgcacc gtcaccccga ggaagaggtg gttgcgctgc ggcgtcggat
      661 catccagcag cgacgagatc ggtttttgtc gatcgataaa tccgtcgagc tgtttgcgcg
      721 ctgcttcgtt gcgggaaaag tagatgctct ggttttccac ctgcttgtgg ttccagaccg
      781 ccgggtgctc gaccgacgct tccaggaaca gcggggcacc gtccggtcca acaagctgtc
      841 ctgcgcagtg ctggccaagc gcgagcagct tctgccgcac ctcaaggcgc tcacggttgt
      901 agacgtttga cttccacttg cgcggctcat agcacgcaaa gtcattctgg gtcagcccga
      961 cgaacatagc gcggagtatg gggtttggcg gtagggtgtg tcaacgcgcg cgacgcaagt
     1021 tacgccggcg gatcggacgc tttcgactgg ctggctttag cgacagcttg cgcactggct
     1081 actggcgtcg ctgcactcgc gttcggcgtt tttgcagctc cgctggcttg gctcgtccgc
     1141 gattttttcc gacagggtgc agattcggcg cgaatagccg cagatcgact gcgcggccac
     1201 acagacaccg tcgcagcgac cggctggagc cgactgggcc gcgcttggtg acatcgcggg
     1261 gactgcatgt gcagacagtg acccgctcgc gacaatgggg atctggctgc ggcggctggc
     1321 gatgttgctt tccagtcggg tgatttcgct gcgtgcctcg gcgacgtcgg gggcaatgct
     1381 cggtctgtgc gcacagccga aagaaagcat gcatcccgca accagcacag cgatcgccaa
     1441 accgaatcgg tccgccgagt gaacacgaaa acaaggcaga aggcgcatcg ggttagtgct
     1501 cctctgtaaa ctcgggctgg gacaaaagcg tccgcaggag gtggcgatcg atggcaatgg
     1561 ccctttcgag ggcacgcacc gcttccttgc gtgcttcctt ttgggcaccc ggatcgcttt
     1621 gccgtttggc ccgcgtttcg tagatttctg ctgccactga ctcgaggttt gcacggaaaa
     1681 agctatcgcg ggtggccaac tttcgtccgg cctcgacttg ggccagtgct tgccgttcgg
     1741 cttcttcgcc gccttgcgaa aggtgcagcc gagccagccg ggcatgggca tcgagcagtg
     1801 tgtcttctcc gtcggtattt cctgtcggaa agcggatcgc cagcacctcg cgaatgtccg
     1861 cgatcgcggc aggcacttcg ccatccaaaa ggtggatgtc tgcccgccga tgtagcgcgc
     1921 gtgcttcgct gagcatcagc agcatggctt cgtcgctgcg cccgaccaaa tcatcgcgac
     1981 tacaggtact cagtagcagc agcatcgcga gcagagcgct ttgccagctc gacggcgtca
     2041 ttgcactttg tctccttctt tcgcagcgtt tggggacacg acgcgatgat gacgattgac
     2101 cagtgtgacg ccgctgcgca ggctcagaat ccgtcggtga cgcagccttt cttgcagcaa
     2161 cagatcgagc ctggctgtcg gactttcgcc tcggcgcagc gattcacgca acagccaggt
     2221 ggtctgattc atcgtcatgc tgggcggaga tcgcagcttg ctgccttgat ccaagatcgc
     2281 ccacgacacc atcagcagca cggcggcccc aagcaaaagc cccccggttc cggtcagcgt
     2341 gcgactgagc ttctgcatcg tcgagctgcg cagctcggct tgcgatcgct tggccgccac
     2401 aagctcgcgg gcagagcgag ccaagccgcg ggcgcgaact tcaccgagcg actccatttc
     2461 cgtcggaaaa cgcagatgtg cagccagcca gcgggcctcg gcgctgcgtg gggaaagtgg
     2521 gtgcagcgga tcggaatcgt gcttgtcttc ggtttcgcgg agcgcttcac gcagggactt
     2581 ggcggcgcgc agttcatcgc ttcgcggcgg cgcatccgga tcgtcgctgt gcgttctgtg
     2641 atcgacctgg ccttcgcgat catcctgcat cgggttctcc ataggctttg cggaaggatc
     2701 gaaccgcccg cagtagcagc acatcaaacg tctcgacgga cacaccgagc gccgcggcac
     2761 acgttgcccg cgatttttct tcacacagcc gcagccgaat cgccgtcgcg tagcgctcgt
     2821 gcagaacctg aagcgtggct tcgattcgct gctgagcgat cgtccgttct tcctgggccg
     2881 aaagcagaag cggcgcgctt gcgtcttcgc tttcactcag ttctgccttc agtgcctgac
     2941 agagccgctt gcgccgtccc tgagcgcggt gaaagtcgat cactttgtga aaagcgatct
     3001 gccgcagcca gtgaaagatg ctgcgctgct gaaacgtata ggtggatatt cgttccagtg
     3061 ccgtcacaaa cgtgtccttc agaatgtcct cggcctgagc acgctctccg acgcggccca
     3121 agatgcccat aaacagtgcc tcggcgtgga cagcgagaat cggttccagt gcagccagat
     3181 cgccagactg ggctttttcg acggcgcgct tttcccgcgc aagcagggcg ctcctttccg
     3241 tcgagtcatc cgggcctgtc cggtcggggt tctgcacgag cgtggtactc cgtaaaaacg
     3301 cgcgaaagaa ttggacctta caggcgtgac acagcgaaat ttgccgtcgg taagtctgcg
     3361 aactggaccc gaaaagcgat cgatctactt gagggtcagc gagaaggccg cgaccgtctt
     3421 ttggcaggtc ttacactcgg ggaaacggat cgactgcatc ttggcgaatg cgcacgaagc
     3481 caccggcgtg ctggttgatc cgttgacacg gaccgacgat acagagccgg ttcccttgac
     3541 gatgaactcg aggtcgatct tcttggtttg cgggctgcgc gcggcttctt ctcgcagaca
     3601 gccagacaac accgaaaagt tcgacatcat cgtcttctga atgtcttccg ccgacagcgt
     3661 gtcgccgccg tcgttaaagt cgacgctctg cgagtcgtca aacgccccgt tcttcggtcc
     3721 cgccttcttt ttctttttgg gcggcgccgg cggcatcggc tgcatcgcga tctgaagacc
     3781 cttgagcgga tcgtcagtaa actgcggccc cgtctgcttg accacttgcg gcttcggtct
     3841 tgtcaccaac cagacgccga cgccagatcc cgccaccacg accgccgcga agatgcccgc
     3901 cagcattttg acgacggcgc ggttgtactt ggcgcgctcg gcctcctctt tcatctggcg
     3961 gtcgagttcc atgtgcttgg cggtccactc tcgggccatt tgcccaagca gcgggtgatc
     4021 cgcgacgcga cgtttgtcgt tcgtctcggt atccgtgatc gtgtgttccg ccgagatcga
     4081 tccgcgctcg atctgcgctt tgacgtcgcg caacgagaac ggaccgtagt ccatgcgatc
     4141 ttctttggtg atcagccagc gctcgtgctc gtcgtcgatt tcacctttgc ccttgggggg
     4201 cggaaggccc ggcatgctgc ccgcaggacg catcgccgaa atcgatccgc tgccgttgcc
     4261 ctgcatcggc ggcactgctc caccgtcggg accacggaga cctggcaggg ccggtccgct
     4321 cgacggttgc gcgccgctcg gtgcctcgcc cgcaccgcct tcagttccag tctgttcctt
     4381 cgccgaagtg tctgacatgt ctctctggtg gcggggttcc gctctgtcgc ccgtccagaa
     4441 ccgaacctgc cgctcctcaa tgacgattcg cacgtcaaaa aagggctacc ctgtacccct
     4501 aataaggctc tcgggcggag cgcctcgcgt ccatagtacc gattctggac agacccggtc
     4561 aacgccggcc ttttgtgcgt ggccatctct ggccgagttc ggtatggttg gaccattttt
     4621 gccgggttgg cacgaaaacg gatgtttctc gacgcaaggt cggcgtttgt gtgcagcgcc
     4681 agggaaacga gtgcttgaca ccgaaatgtc gcagatggta tttgcccgcc tgcgctattg
     4741 gggcgtcgac aagcggtaag tcacgggtct ttggaacccg cattcgtagg tt
//
LOCUS       DAORGN010000288         4712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9456_length_4712_cov_5.981318, whole genome shotgun sequence.
ACCESSION   DAORGN010000288 DAORGN010000000
VERSION     DAORGN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9456_length_4712_cov_5.981318"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1144)
                     /locus_tag="PKL17_20395"
     CDS             complement(110..1144)
                     /locus_tag="PKL17_20395"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNK47154.1"
                     /translation="MKVLVTGATGFLGSWVVHKLVESGYAARVLVRKSSKLDNLQGLP
                     SDKVETAYGDVLDGDSVRAALAGCEAVIHTAGVPHFNPDNPDHMYRVNATGAGIVMEA
                     ALSAKVQKAILTSSVAAIGGSFSVRTATEDTPVNADDIGLDYSRSKLHGEREAQKAAA
                     KGLPLVILRPAVILGPNDILQSSTSTILAFVRRKMPVYVEGRPSFGDVREMAAAHVAA
                     LHKGRPGQVYILGGNNVTLTELLTAIEDLTGIAPPPRVPYPLAYSVAGVLEYMARLQK
                     QHASLSRQLAKAGHMCTWVSSDKAKAELDYTIRPLADSLRDTIRFFLTTGRLKPSTAK
                     LRALADNSRN"
     gene            complement(1146..1937)
                     /locus_tag="PKL17_20400"
     CDS             complement(1146..1937)
                     /locus_tag="PKL17_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47155.1"
                     /translation="MTGIVTKFDGIGMALAAALHGGILMYLYLAKPPAKIAPTVVEVE
                     FRKPKEPPPPPPPETKPEPPKPPEPPPPEPPKKIVKRQPKPAEAPKPSTPPPAEPPKE
                     PPKPVFGIDASQTGGQGISVAVGNTTMADPSKRPKVTEVPPLPASSAPGGSEYRPVAE
                     ENLAKLPEPDSDDCGPSMKEKWSRSETHAQGLEGKVILRIELDERGKVRAIKKIKGLS
                     AEVDNIAIGFLRFDPRCKFKPAVGKDGKPVPFVIERYTVNFENEN"
     gene            complement(1934..2374)
                     /locus_tag="PKL17_20405"
     CDS             complement(1934..2374)
                     /locus_tag="PKL17_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNK47156.1"
                     /translation="MAGSAPSGGDEDGGGMIADINVTPLVDITLVLLIIMMVAAPMIA
                     NNPSIKVELPKAASGDETQKTTLSLTLTKKPNGEGGYDLFLNGEKVDEPKAKVQITDL
                     VGKNKDIQAVIAGDKGIAYGDVMHIVDLVKTLGVTKFAMATDGT"
     gene            complement(2374..3048)
                     /locus_tag="PKL17_20410"
     CDS             complement(2374..3048)
                     /locus_tag="PKL17_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNK47157.1"
                     /translation="MNLTQKLLAFTLLGAEWVLWLLIFLSVISLAIAIERGLYYFSLR
                     ADFAQLADDLRKLLLDHRFVESRKRADAEKGLEAEVARAGLAEAERGADAAEAAMLGA
                     KARIKLRLERNLAFLGTLGSNAPFIGLFGTVLGIIKAFRDLAANQSGGIAIVMAGISE
                     ALVATAVGLLVAIPAVVFFNFYNRRVRAITSHIDAVHQVILAALRAESTHHSEAEPKA
                     KAAEAK"
     gene            3331..>4712
                     /locus_tag="PKL17_20415"
     CDS             3331..>4712
                     /locus_tag="PKL17_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HNK47158.1"
                     /translation="MRDHLYLRLALRVTGFLERMLPDAFPFALLAMAVAVLLGALWAG
                     AGFWQMCDGFGKGFFSLLGFTLQMALVIILGHVVASAALIRRLLDRLADVPKTAKGAV
                     VQVGFLAMLSSWLNWGFSLVFSAMLALATARRFAARGQAVDYRALAAASFLGLGSIWA
                     QGLSGSAALQMATPGSLPPGLLGHGIPLSQTIFLWQSLASVAIEITVVTGLLYLVAPP
                     EAHAVSAEALGISLSPPNSLALPVESLRPGEKLEHSPWLLVPFVLLSAAYLVQTLRTA
                     PSKLEAVSLNTINLTLLLFGALLHKTPARLQQAFAEATSAVWGVILQFPFYGGIAGIL
                     AAAGLNTKIAGLFVGAASQSTLPPLVAAYSALLGIFVPSGGSKWLIEAPYVLAAAERL
                     HVHAGWMVATYDLGEALANLVQPFWMLPILGLYKLKARDVMGYTLIVFAVLCPLVLLL
                     TYALGLTLQG"
BASE COUNT          739 a         1460 c         1479 g         1034 t
ORIGIN      
        1 aagagaggaa caagcaactt ttgtggcaac ttttgtggcg gttttcgggg cggctttttg
       61 cgctgcgaaa caggcaggtc ggcccaggtc gggcgggccg cggcgcggct tagttgcggg
      121 agttgtcggc gagggcgcgc agcttggcgg tggagggctt cagccggccc gtggtcagga
      181 aaaagcggat cgtgtcgcgc agcgagtcgg cgagcggacg aatcgtgtag tccagctcgg
      241 ccttggcctt gtccgatgac acccaggtgc acatgtgacc cgctttggcg agctgtcgcg
      301 acaggctggc gtgctgcttt tgcagccggg ccatgtattc cagcaccccg gccaccgaat
      361 aggccagcgg atagggcacc cgcggtggcg gcgcaatccc ggtcaggtct tcgatcgccg
      421 tcagcagttc cgtcagcgtg acgttgttgc cgcccaagat atagacctgt ccgggccggc
      481 ctttgtgaag cgcagcgacg tgtgccgccg ccatctcgcg aacatcccca aagctgggac
      541 ggccttcgac gtagacgggc attttccgac gcacaaacgc caagatcgtc gatgtgctcg
      601 actgcaagat gtcgtttggg cccaagatca ccgccggacg caagatcaca agcggcagac
      661 ctttggctgc ggctttttgc gcctctcgtt cgccgtgcag cttgctgcgg ctgtagtcga
      721 gcccgatgtc atcggcgttg accggcgtgt cttctgtcgc ggtgcgcaca gaaaagctgc
      781 caccaatcgc ggccaccgac gacgtgagga tcgccttttg taccttggca gacagcgccg
      841 cttccatcac gatgccggcg cccgtcgcat tgacccggta catgtgatcc ggattgtcgg
      901 ggttaaaatg tggcaccccg gcggtgtgga tcaccgcttc acaacctgcc agtgcggcgc
      961 ggaccgaatc accatcgagc acatcgccat aagcggtttc gaccttgtcg ctcggcaagc
     1021 cttgcaggtt gtcgagcttg ctcgacttgc gcacaagaac ccgcgcagcg taacccgact
     1081 cgacgagctt gtggaccacc caagacccga ggaagcctgt cgcccccgtc accagtactt
     1141 tcatgtcagt tctcgttttc gaagttgacg gtgtaacgct cgatcacaaa cggcacgggc
     1201 ttgccatctt tgccgacggc aggtttgaac ttgcagcgcg gatcgaaccg caaaaagcca
     1261 atcgcgatgt tgtcgacctc ggcgcttagt cccttgatct tcttgatcgc gcggaccttg
     1321 cctcgctcgt cgagttcaat gcgcaagatg accttgcctt ccagtccctg cgcgtgtgtt
     1381 tccgaccgtg accacttttc cttcatgctc ggtccgcagt catcggagtc gggctcgggc
     1441 agcttggcga ggttttcctc ggccaccggg cgatattcgc tgccaccggg cgcactcgaa
     1501 gcaggcagcg gcggcacctc tgtcactttc gggcgcttgc ttgggtccgc catcgtcgtg
     1561 ttgccgacgg ccaccgagat gccttgtccg ccggtctgac tggcgtcaat accgaacacc
     1621 ggcttgggcg gctctttggg cggctctgcc ggcggaggtg tcgatggctt gggcgcttcc
     1681 gccggcttgg gctgccgctt gacgattttc ttgggcggct cgggcggcgg tggctccggt
     1741 ggcttgggcg gctccggctt tgtctcgggt ggcggcggtg gcggcggttc cttcggcttg
     1801 cggaactcca cttccaccac cgtcggcgca attttggccg gtggcttggc cagatacaaa
     1861 tacatcaaga tgcccccgtg cagcgccgcc gccagggcca tcccaatgcc gtcaaacttg
     1921 gtgacgattc cggtcacgtc ccgtctgtcg ccatcgcgaa tttggtcact cccagcgtct
     1981 tgaccagatc cacgatatgc atcacatccc cataagcgat gcctttgtca ccggcgatca
     2041 ctgcttggat gtctttgttt ttgccgacga ggtccgtgat ctggaccttg gcctttggct
     2101 cgtcgacttt ttcgccattt aagaacaggt cgtagccgcc ttcgccgttt ggcttcttgg
     2161 tcagcgtgag cgacagcgtg gttttctggg tctcatcgcc actggccgct ttgggtagct
     2221 cgaccttgat cgacggattg ttcgcaatca tcggcgccgc caccatcatg atgataagca
     2281 gcaccagtgt gatgtcgaca agtggcgtga cgttgatgtc ggcgatcatg ccgccgccat
     2341 cttcatcccc acccgatgga gccgagcctg ccattacttc gcctccgccg ccttggcttt
     2401 cggctcggcc tcgctgtggt gggtgctttc cgcccgcagc gccgccaaga tcacctgatg
     2461 taccgcatca atgtgcgacg tgatggcgcg cacccggcgg ttgtagaagt taaagaacac
     2521 gacggcggga atggcgacca aaagtcccac tgccgtcgcg accagtgctt ccgagatacc
     2581 ggccatgacg atggcgatgc cgcccgactg gttggccgcc aagtcacgga acgccttgat
     2641 gatgccaagc accgtaccaa acagcccgat aaacggagcg tttgagccca gcgtgccaag
     2701 gaaggccagg tttcgttcga gccgcagctt gatgcgtgcc ttggcaccca gcatcgccgc
     2761 ttccgccgcg tcggccccgc gctctgcttc ggccagaccg gcccgcgcga cttccgcttc
     2821 caggcccttt tcggcgtcgg cccgcttgcg cgactccacg aagcgatggt ccaaaagcag
     2881 cttgcgcaga tcgtcggcga gctgggcaaa gtccgcccgc aagctgaagt agtacaggcc
     2941 acgctcgatc gcaatcgcca gactaatgac cgataggaag atgaggagcc acagcaccca
     3001 ctcggcgccc agcaacgtga aggcaagaag tttctgtgtc aggttcatcg gactcgaagc
     3061 tctccaaggt gcgctagcga tgtttgagat gccttctggc tgccgccgca ttcaagacgg
     3121 acatcgtttc tgtcctgtca caagcggcgt ctagcaagtc ggccatttct taccgaggag
     3181 tgtagcccag ctttgcaagc ccgcaaccgt aagttggcgg gaacatttgt gctttggctg
     3241 gttttgccgg tccagcatcg ccgctgtggg cgtttggatg gctgcctggg gcggcggtct
     3301 gcgcgggccg tgcccatgcg aacataagac atgcgagacc atctgtatct gcgcctggcc
     3361 ttgcgggtga ctggcttttt ggaacgcatg ctgccggacg cttttccgtt tgcgcttctg
     3421 gcgatggcgg tggcggtgct gctcggcgcg ctgtgggcgg gggcggggtt ttggcagatg
     3481 tgcgacggct ttggcaaagg cttcttttcg ctgctcggct tcaccctgca gatggcgctt
     3541 gtcatcattt tgggccacgt cgtcgccagt gcggcgctga ttcgtcgcct gctcgatcgg
     3601 ctggcggacg tgcccaagac ggccaaaggc gccgtcgtgc aggtcggatt tctggcgatg
     3661 ctgtcgtcgt ggctaaactg gggcttttcg ctggtctttt cggcgatgct ggcgctggcc
     3721 acagcccgtc gctttgcggc gcgcggtcag gcggtcgact acagggcctt ggccgcggcc
     3781 agctttttgg gactgggcag catctgggcg caggggctgt cgggttcggc tgcgcttcag
     3841 atggcgacgc ctgggtcgct gccgccagga cttttgggcc atggaatccc gctgtcgcag
     3901 acgatctttt tgtggcagtc gctggcttcc gtcgccatcg agatcaccgt ggtgacaggg
     3961 cttttgtatc ttgtggcccc gccggaagcc cacgcggtgt cagcggaagc gctggggatt
     4021 tcgctttcgc cgccgaacag cctagctctg cccgtcgaat cgctgcgccc cggcgaaaag
     4081 ctggaacact ccccgtggct gctcgtgccg tttgttcttt tgtcggcggc gtatcttgtg
     4141 cagacgctgc ggaccgcccc ctcgaagctg gaagcggtga gcctgaacac catcaacttg
     4201 acgctgcttt tgtttggcgc gctgctgcac aagacaccgg ccaggcttca gcaggcattt
     4261 gccgaggcga ccagcgcggt ctggggcgtc atcttgcagt ttccgtttta tggcggaatt
     4321 gccgggatct tggccgcggc ggggctcaac accaagatcg ccggcctgtt cgtcggtgca
     4381 gccagccaat cgacgctgcc accgcttgtc gctgcgtatt cggcgctgct gggaatcttt
     4441 gtgccgtcgg ggggcagcaa gtggctgatc gaagcgccct acgtccttgc ggcagccgaa
     4501 aggctgcatg ttcacgcggg ctggatggtc gccacctacg atctgggcga ggcgctggcc
     4561 aacttggtcc agccgttttg gatgctgccg atcctgggtc tttacaagct caaagcccgc
     4621 gacgtgatgg gctacaccct gatcgtcttt gccgtcctgt gccccctcgt cctcttgctc
     4681 acctacgccc tcggcctcac cttgcagggc ta
//
LOCUS       DAORGN010000289         4705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9475_length_4705_cov_5.302151, whole genome shotgun sequence.
ACCESSION   DAORGN010000289 DAORGN010000000
VERSION     DAORGN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9475_length_4705_cov_5.302151"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1137)
                     /gene="hpnD"
                     /locus_tag="PKL17_20420"
     CDS             complement(244..1137)
                     /gene="hpnD"
                     /locus_tag="PKL17_20420"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNK47159.1"
                     /translation="MGQAVDGQKTAAAVTTQSRSNFFFAFLFMPKPRREAIYAVYAYC
                     RVIDDIVDSDDPVAQKESALAQWEDELRAAFFDGQPQHPVAVALAAAHRNFGVRYEDA
                     LLVLRGCQADLHKHRYATWAELYDYCYHVASAVGLMCIELFGCKHPGSRQYAVHLGQA
                     LQLTNILRDIGEDAARDRIYLPQEALSAFGLSDTDILAGRRGTSSLWLVRHLAARARR
                     EYALARESLPASDRRALLPAEIMGSIYFALLEDVEQRGDDVLTNGPRPKLSPRRKMER
                     ALHAVIHSLFPFGHKVFAPAY"
     gene            complement(1201..2124)
                     /locus_tag="PKL17_20425"
     CDS             complement(1201..2124)
                     /locus_tag="PKL17_20425"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNK47160.1"
                     /translation="MKRRAYSLSESYSYCERMVQMRHDSLPVASRYLPESKRPHVFAV
                     YAFARAADDFADEPEFHGQRQSALTNWEDELIRTFHGESDHPIFVALQQTVEACDLPI
                     TLFKDLLAGFRMDLAPRTFVSFDELRTYTRYVSEPLGELIVRVFGLKDPATLNFARDL
                     STGWQLVSFLQNLGRDLPRGRVYIPLEDLRHFGVLPEGTRTAPASAHLLGAGSRAWRD
                     LLRFEVARARALLVRGRPLLDLVGSELRFELLLTYYSGQLLLDRIESLGDAVLQGRPA
                     LSRTDRARVLAKVAARRVPELYFRRSGEEND"
     gene            2446..3126
                     /gene="elbB"
                     /locus_tag="PKL17_20430"
     CDS             2446..3126
                     /gene="elbB"
                     /locus_tag="PKL17_20430"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNK47161.1"
                     /translation="MKRPSVGVVLAGCGFLDGAEIHEAVFTLYFLDRAGFDVHCFAPS
                     RPQHHVVNHLTGEPMAETRNVLVESARIARGKIAPLDTASMSELSALVLPGGYGAAKN
                     LSDLAFRGPDAEVDQDLLRLIGEAVTAHKPILAICISPAVVAAALHKLGKHAELTIGN
                     DEATAQAIEKMGSRHTSCAVTDAVVDEANRIISTPAYMLGPGPKDVAAGIESAVQTLA
                     HWLIGSAS"
     gene            3165..4385
                     /locus_tag="PKL17_20435"
     CDS             3165..4385
                     /locus_tag="PKL17_20435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNK47162.1"
                     /translation="MVDAYILGAVRSPIGRFRGGLREIRPDDLLGQVIARLVERTGVP
                     PETIDDVICGCANQAGEDNRNVARMALLLAGLPQSVPGVTVNRLCGSGLEAVLQAARC
                     IGAGQADLMVAGGVESMTRAPLVMPKPDTAFPSGNVTVFDSSLGWRFPNPKLAALFPL
                     EAMGETAENLAAKYNVSRAEQDAFALRSHEKALAAQKADRFADELVAIDLPPPGKGQP
                     AAKLIQDEGPRGDTSVEKLGKLPAAFRAGGSVTAGNSSTLNDGAAALLIGSAERAKSL
                     GLRPLVRIVSGAVAGVDPRYMGIGPVPAIQKLLARTGIPAEAIGRIELNEAFAVQALS
                     VLRELPTLSQERVNVLGGAIALGHPLGCSGARITCTLTHELRRSGERFGLAAMCIGVG
                     QGIAALLENLDATG"
BASE COUNT          992 a         1407 c         1478 g          828 t
ORIGIN      
        1 ttcgctttca tgtcacgtcc ttgtctgggt cgtccgaaac atcgcccatc gggcgaattc
       61 caagccaacc cgcaaggaac agaccaaagc gaggcgatgt caaaactcgt ttgatgacga
      121 gtccctagcg gtctttggcg aaagaccacc gtgttgcaca cgcaccaaac cgaacagtca
      181 cttgtaccaa tcggtacact ccgtcgaaga tgcccccgac ctgagcgatg gaaagtgaac
      241 cgtttagtac gcgggcgcga agactttgtg accaaacgga aacaaggagt gaatgacagc
      301 gtgtagggcg cgctccatct ttcgtcgcgg cgaaagcttg ggccgaggcc cattggtaag
      361 cacgtcatcg ccacgctgct cgacgtcttc taggagcgca aagtagatac tgcccatgat
      421 ctcggcaggc agaagggcgc gacgatcgga ggccggcagc gactcccgcg ccagcgcata
      481 ctcgcgccgg gctctggccg ccaagtgacg gacgagccac agcgaagacg taccgcgcct
      541 tcccgccaag atgtctgtat cggacaagcc aaacgccgac agggcctcct gcggcagata
      601 gatccggtcc cgcgctgcgt cttcgccgat gtctcgaagg atgttggtaa gctgtagcgc
      661 ttggccaaga tggaccgcgt actgccgtga gccagggtgc ttgcagccaa atagctcaat
      721 gcacatcaga ccgacagccg acgcaacgtg gtagcagtag tcatacagtt cggcccaggt
      781 ggcgtagcgg tgcttgtgca aatcggcttg gcagccacgc aaaaccagca gcgcgtcttc
      841 ataacgcacg ccgaagttgc gatgggccgc agccagtgcg acggcgaccg gatgttgcgg
      901 ctgaccgtcg aaaaaagcgg cacgaagctc gtcttcccac tgcgcaagcg cggactcttt
      961 ttgcgcgacc ggatcatctg aatcgacgat gtcgtcgatg acgcggcagt aggcatagac
     1021 ggcgtaaatg gcttcccgcc gcggcttggg cataaacagg aaggcaaaaa agaagttcga
     1081 gcggctctgg gttgtcaccg ccgccgcggt tttctgacca tccacagctt gtcccacggg
     1141 gttcgaagag gttgagcgcg gccacaaagg gcctctctgc gcgagcaagg cttcggacat
     1201 ttagtcgttc tcttcgcccg aacgacggaa atacagctct ggcacgcgac gggcggccac
     1261 cttggcgagc acacgcgccc tgtcggtacg cgacagcgct gggcggcctt gcagcacggc
     1321 gtcaccgagc gactcaatgc ggtcgagcaa gagctgcccc gagtagtacg taagcagaag
     1381 ctcgaagcgc agctcggacc ccaccaagtc cagcaagggc cgtccgcgca caagcagtgc
     1441 ccgcgcccgc gccacttcga agcgaagcaa gtcccgccag gcacgcgatc cagcgccgag
     1501 cagatgggcc gaagccggag ccgtgcgggt tccctcggga agcacaccaa agtggcgcag
     1561 gtcttcgagg ggaatgtaga cccgtccacg cggcaagtcg cgcccgaggt tttgcaaaaa
     1621 cgagacaagc tgccacccgg tgctcaggtc acgggcaaag tttagcgtcg ccggatcttt
     1681 gagtccaaag acgcggacaa tcagctcccc gagcggctcg ctgacatagc gggtataagt
     1741 ccgcagctca tcgaagctga caaacgtccg cggcgccaag tccatgcgaa agccagccag
     1801 cagatcctta aacagcgtaa tcggcaagtc acacgcttcg acggtctgct gaagtgccac
     1861 aaagatcggg tggtccgact caccatggaa tgtgcgaatt agctcatctt cccagttggt
     1921 gagtgcggat tggcgctgac cgtgaaactc cggctcgtcc gcgaagtcat cggcggcgcg
     1981 ggcaaaggcg tagaccgcga acacatgcgg tcgcttgctc tctggaaggt agcgggaagc
     2041 gaccggcaag ctgtcgtggc gcatctgcac cattcgctcg cagtagctgt acgactcgct
     2101 gagcgaatac gctcgacgct tcacgctgag cacgctgagg tttgacggct cggagacgcg
     2161 gcgaaagagc tggctttccg acgacgaagg gctgctaaaa agctggcggt tgtggatcac
     2221 ggcttgccat cctgcgtaag cgtttgctgc ggagataggc aagaggcgtg cccggcccgg
     2281 actatcccaa agaagtgtgt gccgcgcggt acaaggtcgc gaaaacacac cgcggtggac
     2341 acgtccctgc ccttgggcaa acacccgatg gcaatcccat tcggagatcc gtttcgtacc
     2401 ggctgtacta tcttgcctgc cgaatcaata aaggagtcat ccaagatgaa gcgaccttcg
     2461 gttggcgtcg tgctggcagg ctgtggcttc ctagacggtg ccgagattca tgaagcggtg
     2521 ttcacgctgt actttctgga ccgggccggc tttgacgttc actgctttgc gccaagcaga
     2581 ccgcagcacc atgtcgtaaa tcacctgacc ggcgagccga tggcagaaac ccgcaacgtg
     2641 ctggtggaat ccgcgcgcat cgctcgtggc aagatcgcgc cacttgatac ggcaagcatg
     2701 agcgagcttt cagccctggt gttaccgggc ggctatggcg cggcgaaaaa cctgtcggat
     2761 ctggcgttcc gtggacccga tgccgaagtc gaccaagacc tgctgcgatt gattggcgaa
     2821 gccgtgacgg cgcacaagcc gattttggcg atctgcataa gccccgcagt ggtggccgca
     2881 gcgctgcaca agctgggcaa gcacgccgag ctgacgatcg gcaacgacga ggcaacggcg
     2941 caagcaattg aaaagatggg ctcgcgccac acaagctgcg cagtgacgga cgccgtggtt
     3001 gatgaagcga atcgcatcat cagcacgccg gcctacatgc tggggccagg gccgaaagac
     3061 gtcgccgccg gcatcgagtc agcggtgcag acgttggcgc actggctcat cggcagcgcg
     3121 agctagttgc acggcgcggg acgatggcgc tatcgtgccc gtccatggtc gacgcataca
     3181 tcttaggtgc tgttcggagt ccgattggtc gctttcgggg cggactgcgt gaaatccgac
     3241 ccgacgattt gctggggcaa gtgatcgcgc gtttggtgga acggacgggg gttccgcccg
     3301 agacgattga tgacgtgatc tgcggctgtg caaatcaagc gggcgaagac aaccgcaacg
     3361 tggcccgcat ggcgctgctg ctcgctgggc tgccgcagtc ggtgcctggc gtgacggtca
     3421 accggctgtg tggctcgggg cttgaggcgg tgctacaagc ggcgcgctgc ataggcgcag
     3481 gtcaagcgga tctgatggtg gccggtggcg tcgagtcgat gacgcgagcg cccttggtga
     3541 tgccaaagcc ggatacggcg tttccgtcgg gcaacgtcac ggtatttgat tcgtccttgg
     3601 gctggcgttt tcccaatccg aagctggcgg cgctgttccc tctcgaagcg atgggcgaaa
     3661 cggcggaaaa cctcgccgcg aaatacaacg tgagccgcgc agaacaagat gcattcgcgc
     3721 tgcgcagcca tgaaaaagca ctggccgcgc agaaagcgga ccgttttgcc gatgagctgg
     3781 tcgccatcga tctgccaccg cccggcaaag gacaaccagc ggccaagctg attcaagatg
     3841 aaggaccgcg cggggacacc agcgtggaaa aactcggcaa gctgcccgca gcattccggg
     3901 ccggcggttc ggtgacggcg ggcaattcat cgacgctcaa cgatggggct gcggcgctgc
     3961 tgattggatc tgcagagcgt gcgaagtcgc tgggcttgcg tcccctggtt cgcatcgttt
     4021 caggcgcggt ggctggtgtc gatccgcgct acatgggcat tggacccgta ccggccatcc
     4081 aaaagctgct tgcgagaact ggcattccag cagaagcgat cggtcgcatc gaactaaacg
     4141 aagcgtttgc ggtccaagcg ctgtcggtgc tgcgcgaact tcccacgctg tcgcaagagc
     4201 gcgtcaatgt gctgggaggt gcgattgcgc tgggacatcc acttggctgc tcgggcgccc
     4261 gcatcacctg cacgctcacc cacgagctgc gtcgatcggg cgagcgcttc ggtcttgcgg
     4321 ccatgtgcat tggcgtcgga caaggcatcg ccgcgctgct ggaaaaccta gacgccacgg
     4381 gctagcggtc catcactttg tccgacaagg cggcaacctc gcgacaaatg ccgcacttgc
     4441 cgtaaggacc atcgtgataa gatagccaat tgtaaaatgc agcctctttc ttcgggacaa
     4501 aacaccgtcg caaaggacgc catgacgact ctccgtggta cctcttcccc tgcggccaaa
     4561 aggcactcat tccgccaggg attcgccttc atgaccctaa cctcgctcgt cgcagttggt
     4621 accgcgacgc ccgccctggc cgccgaaaag caggttgtca tcttcgaacc gaaagtcgaa
     4681 ggcggcaaca ttggcgacct cgaaa
//
LOCUS       DAORGN010000290         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9492_length_4698_cov_5.499462, whole genome shotgun sequence.
ACCESSION   DAORGN010000290 DAORGN010000000
VERSION     DAORGN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_4698_cov_5.499462"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKL17_20440"
     CDS             <1..914
                     /locus_tag="PKL17_20440"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK47163.1"
                     /translation="KMDTPDAQLTRAGTVFGTPAYMSPEQARGVKLSPQSDVYACGIV
                     LYEMLTGKPPFEAALPMEVVMMHLRQKPAPLVGFPEPIVRVVNKALEKTPDRRQQSAD
                     ELHREAVECLDTLFPKQTPSAGMSAVTDAQRVSMQAPTVPPQQVPQLQPPAAATSFGS
                     GPPPGAPPPMAPPMAPPTMAPSGAPPMAPPQGSQFGGSAAPPIATGAEQRTMLATPAP
                     NIPNPGGFGGGGPGRTQGMDGSHATMFVAGPAGGMTLTPQALALRQQILAAKPRRTES
                     PLPKPLFWVSWALIGMSFGFLLHIIWN"
     gene            918..1538
                     /locus_tag="PKL17_20445"
     CDS             918..1538
                     /locus_tag="PKL17_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47164.1"
                     /translation="MKFVLGGDEPVSPQDRAEIDQRQQRIRQEADPGILARIHRIEQH
                     PMVPRPRELPRALRGMSTHFDDPLRQWHVGFLAVNDFEIPYEMARDLKECVPQSILRD
                     IFRPTTNFEFMLEPLEIEKDRTGAEAMKEAKAALLRDPVPVIESGVSAVTREQRRRRR
                     FVGRPWQTMIADDQPRQYSRVEGISYREVSEDGSPLEEGTEDGEEE"
     gene            1611..2666
                     /locus_tag="PKL17_20450"
     CDS             1611..2666
                     /locus_tag="PKL17_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47165.1"
                     /translation="MNPPPSDKIRTNLLRLLGGGLSLPAMVSLAAVGEFLLFRLLQPL
                     LRLSPGIFPDWLSGSLIAMGTFSAHFAGVLSLCALLALLLVAMRERGIASHPMGRLGL
                     GMMSLFFVSLASLEMLLPRFISASIGLLRAQWLLQTSSLCISVLLILAVLPRRTASRL
                     HKIGSLLLLIPPLLLLESQWHIMGSRAFMQRLSLVLLVPGYGATLSAACLGLCSLLFL
                     LGRPWNWVKDSLALMATSLVVGGMTMLLVLAPAAAARLIYIGFDLQLPHQPLAQGVYI
                     LSLASWSLSVGSLLLRHGALRLRGFGVLLVGLAGAQPRMIHQETFFLVGLLCLLESLL
                     IDREHSSDAIEPIGRNL"
     gene            complement(2638..3444)
                     /locus_tag="PKL17_20455"
     CDS             complement(2638..3444)
                     /locus_tag="PKL17_20455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK47166.1"
                     /translation="MRRLRIGPKILRVRDEPEPPPTHPDAPPPICLIHGAGMSGVVWT
                     DAVRKIAPSRRVLAPDLPGHGQSDPWHEQSIDLYRDAVGTMCAHLKVPKIVVVGHSMG
                     GAVALRCALAWPERISALVLVASAARLDVPDELWQSLPRELPEGDDTQVEAMPPSLAA
                     LAFSPATHRDVRERWQAVAMQASRKTVIGDFALCRRLDLRAPLAKLNMPTLLMGGSDD
                     LLVSKEQLSETQRGIAGAEMLLLPDAGHLPMLEQPGPFVAALRDFVQSVR"
     gene            complement(3492..4463)
                     /locus_tag="PKL17_20460"
     CDS             complement(3492..4463)
                     /locus_tag="PKL17_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNK47167.1"
                     /translation="MGNVLVFLEQADRRLRPAALSAISLSRAIHKSSGGKLLFVLIGD
                     SVADAAKEAAQFGADGVFTVEAPQLKHYLAETYAPIIARIAKEQGASIVAGCATSVGK
                     DLLPRVAGILDAGVAPDVSGVISAKQYRRATNAGNALQTLEVLSDLVVVSGRQTDFPR
                     AQPSGGSSPIAAVAAGAIDAAGAEFVELQTQKRDRPDLTEARVVISGGRGMKEGKNFA
                     VLEQVCDILGGALGASRAACDAGMVPNDLQVGQTGKVVAPDLYVAVAISGAIQHLAGM
                     KGSKVIVAINKDAEAPIFQVADYGFVGTWEKAVPELVTELKKTKSAS"
     gene            complement(4473..>4698)
                     /locus_tag="PKL17_20465"
     CDS             complement(4473..>4698)
                     /locus_tag="PKL17_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003294894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNK47168.1"
                     /translation="TFAYAEGPRYATLKGIRAAKDKPLEQLQLAQLGVAPGAKTKVLK
                     VEMPPARQAGKKVGSVAELVEKLNKEAKVI"
BASE COUNT          822 a         1419 c         1513 g          944 t
ORIGIN      
        1 caaaaatgga tacgcccgac gcgcagctga cgcgggctgg cacggtgttc ggaacacccg
       61 cgtacatgag cccggagcaa gcgcgcggcg tgaagctgtc gccgcagtca gacgtatacg
      121 cttgcggcat tgtgctttac gaaatgctga cgggaaagcc gccgtttgaa gccgcactgc
      181 cgatggaagt ggtgatgatg cacttgcggc agaagccggc gccactggtt ggttttcccg
      241 aaccgatcgt gcgggtggtg aacaaggcgc tcgaaaagac gccagatcgt cgccagcaga
      301 gcgccgacga gcttcatcgc gaggccgtcg aatgtctgga tacgctgttc ccgaagcaga
      361 cgccgagcgc gggcatgtcg gctgtgaccg atgcgcagcg cgtcagcatg caggctccga
      421 cggtgccgcc gcagcaagtg ccgcagcttc agccacccgc cgccgcgact tcgtttggtt
      481 cgggtccgcc cccgggggca ccgccgccga tggccccacc gatggcccca ccgacgatgg
      541 ctccatctgg agcgccgccg atggcgccac cgcaaggatc gcagtttggg ggctcagcgg
      601 caccaccgat cgcgacggga gcggagcagc ggacgatgct cgcgaccccg gcgccgaaca
      661 ttccgaaccc aggtggtttt ggtggtggcg gaccgggacg aacgcaaggg atggacggca
      721 gccacgccac gatgttcgtc gctggaccag cgggcggaat gacgctgacg ccgcaagcgc
      781 tggcgctccg tcagcagatc ctcgcagcca agccgcggcg aaccgagtca ccgttgccca
      841 agccgctgtt ttgggtgagc tgggcgctga ttggcatgtc gtttggcttt cttttgcaca
      901 tcatctggaa ctaagcgatg aagttcgttc ttggtggcga cgagcccgtc tcgccgcagg
      961 accgcgccga aatcgatcag cggcagcagc ggattcgtca ggaagccgat ccggggattt
     1021 tggcgcggat ccatcgcatc gagcagcatc cgatggtgcc gcgtccgcgt gaactgccgc
     1081 gagccctgcg cgggatgtcg acgcacttcg atgatccgct gcgacagtgg cacgtcggtt
     1141 ttttggccgt caacgacttc gaaattccgt atgagatggc gcgcgatctg aaagagtgcg
     1201 ttccgcagtc gattctgcgc gacatctttc gtccgacgac caacttcgag ttcatgctgg
     1261 aaccgctgga gatcgaaaaa gatcgaaccg gtgccgaagc gatgaaagag gcgaaagccg
     1321 cgcttctgcg tgatccggtg cccgtcatcg agtcgggtgt gtcggcagtg acgcgcgaac
     1381 agcggcggcg tcgtcgtttt gtcggcaggc cgtggcagac gatgattgcc gacgatcagc
     1441 ctcggcagta ttcccgcgtc gaaggcattt cctatcgcga agtcagcgaa gacggatcgc
     1501 cgctcgaaga gggaaccgag gacggcgaag aggaatagcc gccagcgtcg aaagtggccg
     1561 acgtagggtt ttgcttgctc gacgtctaag gcgcggctac cgtgtagaca gtgaacccgc
     1621 caccgtcgga caaaatccga accaatcttc tgcgactgct cggcggcgga ctctcgcttc
     1681 cagcgatggt ttcgctggca gcagtgggag agtttctgct gtttcgtctg cttcaaccgc
     1741 ttttgcggct gtcaccaggg atttttcccg attggttgag cggttcgctg attgcgatgg
     1801 ggacgttttc agcccatttt gctggcgttt tgtcgttgtg cgcgctgctg gcgctgctgc
     1861 tggtggcgat gcgtgaacgc ggcattgctt cccatccgat gggtcggctg ggacttggca
     1921 tgatgtcgct gtttttcgtg tcgctcgcgt cgctggaaat gctgctgccc cgttttattt
     1981 cggcgtcgat tggtcttttg cgggcgcagt ggctgctgca gacttcgagc ctgtgcattt
     2041 cggtgctgct gattttggcg gtgttgccgc gtcgcaccgc cagccgtctg cacaaaatcg
     2101 gaagtctgct tttgctgatt ccgccgcttc tgctgctgga aagccagtgg cacatcatgg
     2161 gaagtcgcgc ttttatgcag cggttgagcc tggtgttgct ggtgcctggc tacggcgcga
     2221 cgctctcggc ggcctgtctg ggactttgct ccctgctgtt tcttttgggc cggccctgga
     2281 actgggtaaa agacagcctg gcgcttatgg ccacctcgct tgtcgtcggg ggaatgacga
     2341 tgctgctggt tttggcaccg gcggcggcgg cccggcttat ctacattggt tttgacctgc
     2401 agcttccgca tcagccactc gcgcagggcg tctacatcct gagcttggcg agctggtctc
     2461 tgtcggtggg ttcgctgctg ttgcggcacg gagccctgcg gctgcgcggc tttggcgtcc
     2521 tgctcgtcgg actggctggg gcccagccgc gcatgatcca ccaagagacg ttttttctcg
     2581 tcgggctgct ctgtcttttg gaaagtctgc tgatcgaccg cgagcactcg tcagacgcta
     2641 tcgaaccgat tggacgaaat ctctgagcgc ggcgacaaac ggtcccggct gttccagcat
     2701 cggcaagtga ccggcgtcgg gaagtagcag catttcggcc cccgcaatgc cgcgctgggt
     2761 ttcggaaagc tgctctttgg atacgagcaa gtcgtcactt ccgcccatca gcagcgtcgg
     2821 catgttcagt ttggcaagcg gcgcacgcag atccagtcga cgacacagcg caaaatcacc
     2881 gatcacggtc tttcgtgagg cctgcatcgc caccgcttgc cagcgctcgc gcacatcacg
     2941 gtgcgtcgcg ggcgaaaaag ccagggcagc caaagacggt ggcatcgctt cgacctgtgt
     3001 gtcatcccct tcgggcagct cccgcgggag cgactgccac agctcgtcgg gaacatcgag
     3061 cctggccgca ctggcgacga gcactagcgc cgaaatccgt tccggccatg ccagcgcgca
     3121 gcgcaaagcg accgcaccac ccatcgagtg accgacgacg acaatcttgg gcactttcag
     3181 gtgtgcacac atcgtcccga ccgcatcgcg atacagatcg atgctctgct cgtgccatgg
     3241 gtccgactgg ccgtggccag gcagatcggg cgcaaggact cgacgcgatg gcgcgatttt
     3301 tcgcaccgca tccgtccaga cgacccccga cattccggcg ccgtgaatca ggcaaattgg
     3361 tggcggagca tccggatgtg tcggtggtgg ttcgggttca tcacgaaccc gcagaatctt
     3421 gggaccgatg cgaaggcgcc tcatggcagg ggcagaacgt aggcgcccac ggggacgagt
     3481 gcaagtccag cttacgaagc cgacttggtc tttttgagct cagtgacaag ctcggggacg
     3541 gctttttccc acgtaccgac gaagccgtag tctgcgacct ggaaaatcgg cgcttcggcg
     3601 tccttgttga tggcgacgat gaccttgctg cccttcatgc cggccaggtg ctgaatcgcg
     3661 ccggagatgg cgacggcgac gtagagatcg ggggccacga ctttgccggt ctgaccgacc
     3721 tggaggtcgt ttggaaccat gccggcgtcg catgcagccc ggctcgcacc gagtgcaccg
     3781 ccgaggatgt cgcagacttg ctcgaggacg gcgaagtttt tgccttcctt cataccgcgg
     3841 ccacccgaga tgaccacgcg cgcttcggtc agatccggac gatcacgctt ctgcgtctga
     3901 agttcgacaa actctgcgcc ggctgcgtcg attgctcccg cggccaccgc ggcaatcggc
     3961 gagctaccac ccgacggctg tgcgcgcggg aagtccgtct gacggccact gaccacgacg
     4021 aggtccgaga gaacttcgag cgtctgcagc gcattgcctg cgttggtggc gcgacggtat
     4081 tgcttggccg atatcacgcc gcttacgtcg ggcgcaacgc cagcgtcgag gattccagcc
     4141 acgcgcggca gcagatcctt gccgaccgag gttgcgcagc cagcgacgat gcttgcacct
     4201 tgctctttgg cgatgcgggc gatgatcggt gcataggttt cagccaagta gtgcttgagc
     4261 tgtggcgctt cgacggtgaa aacgccatcg gcgccgaact gagcggcttc tttggcggcg
     4321 tcagcgacgg aatcaccaat cagcacaaac agcagcttgc cgccgctgga cttgtggatt
     4381 gcacgggaaa ggctgatggc cgaaagcgca gcgggacgga gacgcctgtc ggcctgttcg
     4441 agaaaaacaa gtacgttgcc catggcatca cctcagatga ctttggcttc tttgttcagt
     4501 ttctcaacca gctcagcgac ggagccgacc ttcttaccag cctggcgcgc cggcggcatc
     4561 tcgactttga gcaccttggt ctttgcaccc ggcgcgacgc caagctgtgc aagctgaagc
     4621 tgctcaagcg gcttgtcctt cgctgcccga atgcccttga gcgtcgcgta gcgcggacct
     4681 tcggcatagg caaacgtc
//
LOCUS       DAORGN010000291         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9503_length_4694_cov_5.209097, whole genome shotgun sequence.
ACCESSION   DAORGN010000291 DAORGN010000000
VERSION     DAORGN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9503_length_4694_cov_5.209097"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PKL17_20470"
     CDS             complement(<1..1906)
                     /locus_tag="PKL17_20470"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNK47169.1"
                     /translation="MSARYLVGIDLGTTNSALAFIDTEEKPRRVRDFPLVQLVGAADV
                     AARPTLASVHHHVGEHELPPESTRLPWTASDQPAPNFVVGELARAQALLLPGRSVLSA
                     KSWLCHPKVDRLAAILPWGGAADVPRLSPVDVSARYLAHMLSAFCHARGCTLQDIDLT
                     LTVPASFDEVARELTVEAARRAALPLSQLQLLEEPQAAFYHWLSQHARLHEEVESGDL
                     IFVCDIGGGTTDFTLISVQKSAAGGGKKIGFSRTAVGEHLLLGGDNMDLLLARQVEQK
                     VGGGKLPAHSFAGLTQACRVAKEKLLGDEPPVSWSVHVAGRGSRLIGGGLHSELFRAD
                     VERAILDGFFPQVGQDVEPTRSRSGLQEFGLPYATDAAVTRYIAAFLRQHKAVPTVVL
                     YNGGVLCSQTIRERILDVLASLGGVRPRLLTNDAPDLAVARGAAYYALSRRGEGLRIS
                     GGAARAYYIEVESPESKKPRHVVLLPRGMEEGSELELPSQPMALLLNRPVRFRLFSSV
                     LREGDAPGQVLDIETSELSELPPLYTVLRHPKSSQQRPVTVTLKARLSEIGTLELWAV
                     AAEKDPDGGVPLKWRLQFDLRQSEGRAAETSPGADKTTNSPSDGEEVSALPAEQAGLV
                     AAAAEQIRAVF"
     gene            complement(1928..3793)
                     /locus_tag="PKL17_20475"
     CDS             complement(1928..3793)
                     /locus_tag="PKL17_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNK47170.1"
                     /translation="MSTQSRYVVGIDLGTTHSVLAYLDTTELSDDGPLPAPTLFAVPQ
                     VVLPGQVEARPLLPSFLYLAGASDFPSRSLDLPWPGGQDRSFVVGELARSHGGQVPMR
                     LVSSAKSWLAYDGVDRTAPILPFAAPTDGPDPVVKVSPVEASARYIQHLRDAWDAQHP
                     GHPLAEQELLITVPASFDEVARELTVRAAQAAGLAKVTLLEEPQAAFYAWLGRHPRSW
                     RDLLRVGDVVLVCDVGGGTTDFSLISVADQSGSLTMERIAVGDHILLGGDNMDLMLAM
                     SVGKRLEREGHKLEPWQRRQLLHGCRFAKEELLGSDRSKESAPVVVLGRGSKVIGGSI
                     KTQLLRSEVEKFLLDGFFPVVDSAARPLAARRMGLQEIGLPFASDPAVTRHLAKFLGD
                     HRLPTALLFNGGVMKGTPLRRRVEQVLSDWAGQTVRALTGADYDHAVALGAAYYGLVR
                     RGHGLRIRGGTARAYYIGVEAAMPAVPGMDPVLHALCVAPRGMEEGTTAEVPGREFGL
                     YVGQPVEFRFLSSSTRKGDAVGDFIEEVSPEHGIEEQASVETVLPAGEGAPPGSQIPV
                     RLISHVSEVGVLELFCVSRDEKRRWKLEYNIRERGAAAGLSDEPELAAFEDTPEL"
     gene            complement(3790..4413)
                     /locus_tag="PKL17_20480"
     CDS             complement(3790..4413)
                     /locus_tag="PKL17_20480"
                     /inference="COORDINATES: protein motif:HMM:NF022273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNK47171.1"
                     /translation="MSRFLLALRCFFAVLFSKRLPSDAVELLPNPAPALPASEPVKQP
                     VAPPEKPPEPVRSEPKPEPTPAVPPASLSTAERDAIRQRGAVQLLALLQREGRLVDFL
                     LEDIDGYSDAQIGAAVRDIHRGCKKGLAEHLQVVAIRPEPDEAVVKIAAGHDPSQIRL
                     VGHVTGKPPFEGSLRHHGWRVKTSSLSDIPAGHDPSVICPAEVEIAG"
BASE COUNT          878 a         1633 c         1377 g          806 t
ORIGIN      
        1 caaacaccgc gcgaatctgc tctgccgctg ccgccaccag gcccgcttgt tctgccggca
       61 gggcggacac ttcttcgcca tccgacggag aattggtcgt tttgtcggcc cccggactgg
      121 tttccgccgc gcggccttcg ctttgtcgca agtcgaactg gagccgccac ttgagcggaa
      181 cgcccccgtc agggtctttt tccgctgcga cagcccacag ctccagcgtg ccgatttccg
      241 acagccgcgc cttcagcgtc actgtcaccg ggcgctgctg cgacgacttg ggatggcgca
      301 gcacggtata aagcggcggc agctccgaca gctcgctcgt ttcgatgtcc agcacctgcc
      361 ccggcgcatc gccttcgcgc agcacgctcg aaaacagccg aaaacgcacc ggacgattga
      421 gcaaaagcgc catcggttgc gacggcagtt ccagctcgct gccttcttcc atgccacgcg
      481 gcaacagcac aacgtggcgt ggctttttgc tctccgggct ctcgacttcg atgtagtagg
      541 cacgagccgc accgccggaa attcgcagac cttcaccacg ccgcgacagc gcgtagtacg
      601 ccgcgccacg cgccactgcc agatcggggg catcgtttgt caaaagccgc ggacgcacac
      661 caccaagcga cgcaagcacg tcgagaatcc gctcgcgaat cgtctgcgaa cagagcacgc
      721 caccgttata caaaaccacc gtcggaactg ctttgtgctg gcgcaaaaat gcggcgatgt
      781 agcgcgtcac cgcagcgtcc gtcgcatacg gcagaccaaa ctcttgaaga cccgatcggc
      841 tgcgcgttgg ttcaacgtct tggccgacct gcgggaaaaa accatccaga atcgcgcgct
      901 cgacgtcggc ccggaaaagc tcgctgtgca gaccgccacc aatcaaccga ctgccgcgcc
      961 cagcgacgtg gacactccag ctcaccggcg gttcatcccc gagcagcttt tccttggcca
     1021 cccggcaggc ttgcgtaagc cccgcgaagc tgtgcgccgg cagcttgccg ccaccgactt
     1081 tttgctcgac ttgccgcgcc aacaaaagat ccatgttgtc gccaccgagc agcaggtgtt
     1141 ccccgaccgc ggtgcgcgaa aagccgatct ttttgccgcc gcccgccgcc gatttctgca
     1201 ccgagatcag cgtaaagtcc gtggtgccgc cgccgatgtc acagacgaag atcaggtcgc
     1261 ccgactcgac ttcctcgtga agccgcgcat gctgcgacag ccagtgatag aacgccgcct
     1321 gcggttcttc cagaagctgg agctgcgaaa gtggcaacgc cgcccgccgc gctgcttcca
     1381 ccgtcagctc gcgcgccact tcgtcaaacg aagcaggcac cgtcagcgtc agatcgatgt
     1441 cctgtagcgt gcagccgcgt gcatgacaaa acgccgacag catgtgcgcc aaatagcgcg
     1501 ccgacacgtc gacgggacta agccgtggca catcggcagc cccaccccac ggcaagatcg
     1561 cagccagccg atcgactttg ggatgacaca gccagctttt cgcagacaaa accgaacgcc
     1621 ccggcagaag cagcgcctgc gcccgcgcca gctcgccgac gacaaaattg ggtgccggct
     1681 ggtccgatgc ggtccacggc agccgcgtcg attccggcgg cagctcgtgt tcgccgacgt
     1741 gatgatgcac cgacgccagc gtaggccgcg ccgccacatc tgccgcgccg acaagctgca
     1801 caagcggaaa gtcccgcacc cgtcgcggct tttcctcggt atcaataaac gcgagcgccg
     1861 agttggtggt gcccagatcg atgccgacaa gatagcgcgc gctcaagggt tagctcccgc
     1921 ctggcgtcta aagctccggt gtgtcttcaa acgcggccag ttctggctcg tcggaaagac
     1981 ccgccgctgc gccgcgttca cgaatgttgt attcaagttt ccagcgccgc ttttcgtcgc
     2041 gcgacacgca gaaaagttcc agcacgccca cttcactgac gtggcttatc agccggaccg
     2101 gaatctggct gcccggtgga gcgccttctc cggcgggcag caccgtttcg accgaggctt
     2161 gctcttcgat gccgtgctcg gggctgactt cttcgatgaa gtcgccgacc gcgtcgcctt
     2221 tgcgggtgga cgacgacaga aaacgaaact cgaccggctg gccgacgtat agaccaaact
     2281 cgcgccccgg cacttcggcg gtcgtgcctt cttccatgcc gcgcggcgcc acacacagcg
     2341 catgcagcac cgggtccatc cccggcaccg ctggcatcgc tgcttcgacg ccgatgtaat
     2401 aggcccgcgc cgtgccacca cgaatccgca gcccgtggcc gcgtcgcacc aaaccgtaat
     2461 acgccgcgcc gagcgccacc gcgtgatcat agtccgcccc ggtcagcgcc cgcaccgtct
     2521 gacccgccca gtccgacagc acctgttcga cgcgacgccg cagcggcgtg cctttcatga
     2581 cgccaccgtt gaacaacagc gccgtcggca gtcggtgatc gcccaaaaac tttgccaggt
     2641 gccgcgtcac cgccgggtct gacgcaaacg gcaggccgat ctcttgcaga cccatgcgcc
     2701 gggccgcaag cggtcgcgcc gcggaatcga cgacgggaaa aaagccatcg agcaggaact
     2761 tttcgacctc ggaccgcaga agctgggtct ttatcgatcc gccgatcact ttgctgcccc
     2821 gtccgagcac caccaccggc gccgattctt tgctgcgatc ggagccgagc agttcttctt
     2881 tggcaaaccg acagccatgc agaagctgcc gacgctgcca cggttccagc ttgtgacctt
     2941 cgcgctctag ccttttgccg acgctcatcg ccagcatcag gtccatgttg tcgccgccga
     3001 gcaggatgtg atccccgacc gcaatccgtt ccatcgtcag gctgccggac tgatcagcga
     3061 cagaaatcag cgaaaaatcg gtcgtcccgc cgccgacatc gcagaccagc acaacatcac
     3121 cgacgcgcag aagatcgcgc cagcttcgcg gatgccgtcc cagccacgca taaaacgccg
     3181 cttgcggttc ttcgagcagc gtcactttgg caagccccgc cgcctgcgcc gcccgcaccg
     3241 tcagctcgcg cgcgacttca tcaaacgacg ccggcaccgt gatcagcagc tcttgctccg
     3301 cgagcggatg gcccggatgt tgcgcgtccc atgcgtcgcg taagtgctgg atatagcgcg
     3361 ccgaggcttc gaccggactg actttcacaa ccgggtcggg accgtcggtg ggtgccgcaa
     3421 acggcaaaat cggcgcggtg cgatcgacgc cgtcatatgc aagccacgac ttggccgacg
     3481 acaccagccg catcggtact tgccccccgt gcgagcgcgc cagttcaccc acgacaaagc
     3541 tgcgatcttg cccgcccggc cacggcagat cgagcgaccg cgacggaaaa tcgctcgcgc
     3601 ctgcgaggta cagaaacgac ggcagaagcg gtcgggcttc gacctggccc ggcaagacca
     3661 cctggggcac cgcaaacagc gtcggcgccg gcagcggacc gtcatctgac agctcggtgg
     3721 tatccaggta cgccagcacc gaatgggtgg tgccaaggtc gatgccgacg acgtaacgac
     3781 tctgggtgct catcctgcga tctcaacttc cgctggacag atgacgctgg ggtcgtgacc
     3841 ggcgggaatg tcggaaaggc tactggtttt gactcgccat ccatgatgcc gcagcgagcc
     3901 ttcaaacggc ggttttcccg tgacatgacc gaccaagcgg atctgcgagg ggtcatgccc
     3961 ggcggcgatc ttgaccactg cttcgtcggg ctcgggacga atcgccacca cttgcaggtg
     4021 ctcggccaga ccctttttgc agccgcggtg gatgtcgcgc accgccgcgc caatctgggc
     4081 atcgctgtag ccgtcgatgt cttcgagcag aaagtcgacc aggcggcctt cgcgctgaag
     4141 cagcgccaga agctgcaccg ccccgcgctg acgaatcgca tcgcgctcgg cagtcgaaag
     4201 cgacgctggc gggacagcgg gcgtcggctc gggcttgggt tccgaccgca ccggctcagg
     4261 tggtttttct ggcggggcga cgggctgctt caccggctca ctggcaggca gagccggcgc
     4321 cggattcggc aaaagctcca ccgcatcgct cggcaagcgt ttgctgaaaa gcaccgcaaa
     4381 aaagcagcgc agagcaagca gaaatcgact cattgtggac tgcttgtagt gctcactcga
     4441 cgcccgtgtc aagcttcgac gcaaacagaa agcccccctg ccccccaact ccataagttg
     4501 gcgcgcggaa atcggtggct ccttgctctg ccgctggttt tcgtttgcag aacttggcgg
     4561 ctccccggtc ttccgctggt tttcgtttgc ggaaatcggc ggctccccgg tcttccgctg
     4621 gttttcgttt gcggaaatcg gcggctcccc ggtcttccgc tggttttcgt ttgcggaaat
     4681 cggcggctcc ccgg
//
LOCUS       DAORGN010000292         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9722_length_4631_cov_7.090035, whole genome shotgun sequence.
ACCESSION   DAORGN010000292 DAORGN010000000
VERSION     DAORGN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_4631_cov_7.090035"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PKL17_20485"
     CDS             <1..755
                     /locus_tag="PKL17_20485"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNK47172.1"
                     /translation="HTTSETDLPSELAMLVALGGDGTMLYGAGLLGDRNVPLLGVNLG
                     RLGFLTCSTPHEARETLLAALDGNLPTEPRHRLRCRLYRLPSDGSVTTPRPEHLLVER
                     TALNDVVVNQPKMARLFELDAFVDGDAVASYRADGLIICTPTGSTAYNLAAGGPILMP
                     NMEALVISPICPHTLTARPLVVHLRSVVEVRPGRDAEQALLTVDGQWSHMLGPHDVVE
                     VTCSDRPLTIFRPLRRSFFDLLRNKLHWGLRV"
     gene            870..1175
                     /locus_tag="PKL17_20490"
     CDS             870..1175
                     /locus_tag="PKL17_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47173.1"
                     /translation="MKKELIQKVLESESAVKAEGKGVFSVNDEVELTVILDVGGQEPL
                     AVPKVKRVTLGAESLLIETYKGDRIYTDGAVGIRALRFSPQDSSKSRGTGFAAAAQR"
     gene            1185..2306
                     /locus_tag="PKL17_20495"
     CDS             1185..2306
                     /locus_tag="PKL17_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNK47174.1"
                     /translation="MSSTQTVYPFGLPAAPGRKRRIAVIAGDGIGPEVVAQALRAVEP
                     LGIPLEIVHFPWSADHYLKTKETLPSGALADLRDNFDAIFFGALGDPRVPDGVVARDV
                     LLGLRFGLDLYINLRPVQLLSEKLCPIKGKTRRDVNFWVLRENTEGPYVQMGGSFKRD
                     TPDEVAISEDLNTRKGVERIIAAAYALANVPHGSSHSTPRVCMVDKANALPHAHGLWQ
                     RTFKAVAATHPSVQSSHLFVDVAAMEFVRTPERFDVVVTSNLFGDILTDLGAAICGGL
                     GVAASGNIHPGRCSLFEPVHGSAPDIAGKGLANPLAAFLTLAMMYDHLGESACGDRLR
                     HAVEVALQSGRVTRDLGGACSTAECGDAVLAALTKPTPS"
     gene            2403..4235
                     /locus_tag="PKL17_20500"
     CDS             2403..4235
                     /locus_tag="PKL17_20500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNK47175.1"
                     /translation="MKIHFIGICGTGMGAVAELLQAAGHEVRGSDEHVYPPMSTQLEH
                     AGIALMEGFADRNLDWQPDVVVVGNICRRDHVEVLAAQQRGIRLTSFPAIVQELLLPG
                     KTPLVICGTHGKTTTSSLAAWTLLNAERDPSYMIGGVPLNMGCNAQIGKGPHFVLEGD
                     EYDCAFFDKKSKFLHYMPQVAILTSVELDHVDIFADLEHIKQAFREFVDLIPETGALV
                     VCASSNHALDCAKSARCRVERYGIVGSCPDDIEWKAEVTTIKPGGRTAFRLHRNGQLY
                     GSFETGLPGSYNLENVVGVIAALANLGLSAAEIAPGIRRFVGVKRRQELRGVAQGVTV
                     VDDFAHHPTAIRETLRGLRGRFGSGRLIAVFEPRSATSRTAVFQNEFAEALAAADEIL
                     IGAVHMPEKAPAGNRLDIELLAADIRKRGVPARALPKTEQILDSLAQRASAGDTVVIM
                     SSGSFDNLHDRLLQKLGDAVQPAGAADVPKVKRLLERVQLPVVGVPDELAELWVLKDG
                     QGEVMGCVALEIYGDTALLRSLAVHPERRGEGLGWMLAEMAQLRVRQRGLLRICLLTE
                     HATDFFAEKFGFRPVPRETLPPEVQSSSEYQRFCGRAVAMALEL"
     gene            4483..4555
                     /locus_tag="PKL17_20505"
     tRNA            4483..4555
                     /locus_tag="PKL17_20505"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4516..4518,aa:Thr,seq:tgt)
BASE COUNT          859 a         1344 c         1510 g          918 t
ORIGIN      
        1 tccatacgac gagcgagacc gatctaccga gtgagctggc gatgctggtt gcgctaggtg
       61 gagacgggac gatgctgtat ggtgccggac ttttgggcga tcgcaacgtg ccactgctcg
      121 gcgtaaacct tgggcggctg ggctttctaa cgtgcagtac gccgcacgaa gcccgtgaga
      181 cgctgctcgc ggcacttgac ggaaacctgc cgacggaacc tcgtcaccgt ctgcgctgtc
      241 gactctatcg cctgccaagc gatggcagcg tgacgacgcc gcgacccgag catctgctgg
      301 tcgaacgcac cgcactaaac gacgtcgtgg taaaccagcc gaagatggcg cgtctgttcg
      361 agctggatgc atttgtcgac ggcgatgcgg tcgcttcgta tcgcgccgat gggctcatca
      421 tctgcactcc gacgggatcg acggcttaca acctggcggc aggcgggccg attttgatgc
      481 cgaacatgga agcgctggtg atttcgccga tctgtccgca cacgctgacc gcgcggcctc
      541 tggtggttca cctgcgatcg gtcgtcgaag ttcggcctgg tcgggatgca gagcaggcgc
      601 tgctgaccgt cgacgggcag tggagccata tgctgggtcc acacgatgtc gtggaggtga
      661 cgtgttcgga ccgtccgctg accatttttc ggcctctgcg acgatcgttc tttgatctgc
      721 tccgcaacaa actgcactgg ggacttcgcg tctgaacgtc gacggaaggc tcttgcaatg
      781 ccctatcgga ccggctgcgc gtggagtttg ccaggccggg cgaattgggt tacttacgcc
      841 gcgctggccc gcatggccaa gggaacaaga tgaagaagga actgatccaa aaagttctcg
      901 aaagtgaatc cgccgtcaaa gccgagggaa aaggtgtctt ctcggtgaac gatgaagtcg
      961 agctgacggt gattctcgat gtcggtggcc aagaaccgct ggcggtgccg aaggtcaagc
     1021 gggtgacgct cggagcggag tcgctgctca tcgagactta caaaggtgac cggatctaca
     1081 ccgacggggc ggtcggaatt cgggcgctca ggttcagtcc ccaggacagc agcaagtcgc
     1141 gcggcactgg cttcgcggcg gcggcacagc gctaggtcct ggcgatgtcg agcacgcaga
     1201 ctgtctatcc gttcggtctt cccgcggcgc cgggtcgtaa gcggcgaatt gcggtgattg
     1261 ccggcgatgg aatcggcccc gaagtcgtgg cccaagcact gcgcgccgtc gagccgctcg
     1321 ggattccact cgaaatagtg cactttccct ggtctgccga tcactacctg aagaccaaag
     1381 agacgctgcc gagtggagcg ctggcggatc tgcgcgataa cttcgatgca attttctttg
     1441 gtgcgctcgg tgatccacgg gttcccgacg gtgtcgttgc tcgcgatgtg ctgctcggcc
     1501 tgcgctttgg gctcgacctg tatatcaacc tgcgtccggt gcagcttctt tcggaaaagc
     1561 tgtgcccgat caagggcaag acgcgccgcg acgtgaactt ctgggttctg cgcgaaaaca
     1621 ccgaggggcc gtatgtgcag atgggcggtt cattcaaacg cgacacgccc gacgaagtgg
     1681 cgatcagcga ggatttgaac acccgcaagg gcgtcgagcg gatcatcgcg gcggcgtatg
     1741 cgctggccaa tgttccgcac ggctcgtcgc acagcacgcc gcgggtgtgt atggtcgaca
     1801 aggccaatgc gctgccgcat gcccacgggc tttggcaacg cacgttcaaa gcagtcgctg
     1861 cgacgcatcc aagtgtgcaa tcgagccacc tgtttgtcga cgtggctgcg atggaatttg
     1921 tgcgcacgcc cgagcgcttt gatgtcgtcg tgacctcgaa cttatttggc gacatcctga
     1981 ccgatctggg cgctgcgatt tgcggtggtc ttggggtcgc agcatcggga aacatccatc
     2041 cgggtcgctg ttcgctgttt gaaccggtcc acggatcggc ccctgacatt gccggcaagg
     2101 gactcgccaa tccgctcgcg gcgtttttga cgctggcgat gatgtatgac cacttgggcg
     2161 aatccgcatg cggcgatcgg ttgcgtcacg ccgtcgaggt tgcgctgcag tccggtcgtg
     2221 tcacccgtga tcttggtggt gcgtgttcga cggctgaatg tggcgacgcc gtgcttgctg
     2281 cactgacaaa accgacgccc agctaactgg gtcctaccga cggcgcgctt ccgttatact
     2341 ggcccaaacg cgaatcagcc tggtccggcg ccgcacgcaa cgcgacgctg atgaggagaa
     2401 acgtgaagat tcactttatt ggcatctgcg gtaccgggat gggcgccgtg gccgagctgc
     2461 tccaggcggc tggtcacgaa gtccgaggct ccgacgagca tgtctatcct ccgatgtcga
     2521 cgcagcttga acatgctggc attgcactca tggaggggtt tgccgatcgc aacctcgact
     2581 ggcagccgga tgttgtggtc gtcgggaaca tctgccggcg cgaccatgta gaagtccttg
     2641 cggcgcagca gcgtgggatt cgccttacgt cgttcccggc gatcgtgcag gagctgcttc
     2701 tgccggggaa gacgccgctt gtgatctgcg gtacgcacgg caagacgaca acctcgtcgc
     2761 tggcggcgtg gacgttgctt aacgcagagc gcgacccgtc gtacatgatc ggcggcgtgc
     2821 cgctcaacat ggggtgcaac gctcagatcg gcaaaggtcc gcactttgtg ctcgaaggcg
     2881 acgagtacga ctgcgcgttc ttcgacaaaa agagcaagtt tctgcactac atgccgcagg
     2941 tggcgatcct gaccagcgtc gagctggacc acgtcgacat cttcgcagac ctcgaacaca
     3001 tcaagcaggc cttccgcgaa ttcgtcgatt tgatcccgga aaccggggcg ctggtcgtct
     3061 gtgcgagctc aaaccacgct ctcgactgcg ccaaatcggc acgctgtcgg gtggagcgct
     3121 acggcatcgt cggttcatgt ccggatgaca tcgagtggaa agcggaagtc acgacgatca
     3181 agccaggtgg gcgcaccgcg tttcggctgc accgcaacgg ccagctgtac ggcagctttg
     3241 aaaccggctt accgggaagc tacaacctgg aaaacgtcgt cggtgtgatc gctgcgctcg
     3301 ccaaccttgg cctgtctgcc gccgagatcg ctcccggcat tcgccgtttt gtgggtgtga
     3361 agcgtcggca ggaactgcgt ggcgtggccc agggcgttac cgtcgtcgac gactttgccc
     3421 accatccgac cgcgattcgc gagacgctgc ggggcctgcg cggacgcttt ggttcgggcc
     3481 ggctgattgc ggtcttcgag ccgcgctccg cgacgagccg caccgctgtt tttcagaacg
     3541 agtttgccga ggcactcgcc gctgccgacg agatactgat cggcgctgtc cacatgccgg
     3601 aaaaggcacc ggcgggaaat cggctcgaca tcgaactttt ggccgccgac attcgcaagc
     3661 gtggcgtccc tgcgcgcgcg ttgccgaaga ccgagcagat actcgactcg ctcgcacagc
     3721 gggcatcggc gggcgataca gtggtgatca tgtcgtcggg cagttttgac aacctgcacg
     3781 atcgactgct gcaaaagctc ggcgatgcgg tgcagccggc tggggccgcc gacgttccga
     3841 aagtgaagcg tctcctggaa cgggtccagc tgccggtggt cggggtaccc gacgagctgg
     3901 ccgagctatg ggttctcaaa gacgggcagg gcgaggtgat gggctgcgtg gcgctggaaa
     3961 tctacggaga tacggcgctt ttgcgatcgc tggcggtgca tccggagcga cgaggtgaag
     4021 ggctgggctg gatgctcgcg gagatggccc agcttcgcgt tcgccaacgg ggtcttttgc
     4081 ggatctgtct gctcaccgag catgcgacgg acttcttcgc tgaaaagttt ggctttcggc
     4141 ccgtcccgcg cgaaacgctg ccgcccgaag tccagagctc gtccgaatac cagcgattct
     4201 gcgggcgtgc ggtggccatg gcgcttgaac tgtagaaaag acagcttgcc tccgcgcgca
     4261 aattgagcga aatttccaac attgttgatc agggccgcgt caggacaaat ccgcctgtct
     4321 ccaggctggc ggaaaagact ggcgcatatg gtccctcgct gaagtctgcg agctgtcgtt
     4381 tgtgacgaca aagacggtcg taaggtagca aaaacaaaaa agtttcttga cagaagccgg
     4441 gaaggagcgg tactactcgc tgcctcgcgg ccgtcggcgc ttgctggcgt agctcacttg
     4501 gtagagcagc tgacttgtaa tcagcaggca gcgggttcga ttcccgccgc cagctctttg
     4561 aaaatctgaa tacgaacgga ggggtgcccg agtggccaaa gggagctgac tgtaaatcag
     4621 ccgcgtctac g
//
LOCUS       DAORGN010000293         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9842_length_4597_cov_5.797226, whole genome shotgun sequence.
ACCESSION   DAORGN010000293 DAORGN010000000
VERSION     DAORGN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9842_length_4597_cov_5.797226"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="PKL17_20510"
     CDS             <1..1926
                     /locus_tag="PKL17_20510"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNK47176.1"
                     /translation="AFRKFATGTKLFSRQGLVGDHFISLALVLSIKNTDERQETLGNI
                     EKAIADIEKSPAPLTRLRKVGQPIVNAYLDNDTRQAGLRYFPLFFLFVVVLNVGLYRS
                     WRALLAFIVTLAICAAFTVGFVGLTHGVFTIVSSLVPMTILIVCTATLVYLHSRYVDR
                     PEDRSVEDHQIFALCNKFLACTASIVASAVGFAALAVSKIRPIREMGIWVAVGLLITW
                     VTVFTLYPALVRILRCPTGQERQIAGAWFTRLVGHLPVFSYRYRYLLVFGSLVLCAIG
                     GIAVFGLPGVVKPMKLETAAIEYIPHESEMYKSTKEMEKLVGGLSITNVWLQGKPGMV
                     SNAKVLRGLMDFQKRLDQEPQIGSTQSVVTLLSMLRYLSTDNDVLPTDDEGLEALTDT
                     LETQLDREPLLQSFVDKGNVSQTHFAVVSRTQDHDEFQQLARVVENHWKASVAAHPAL
                     NDFAMRPVGLAPLQAKISHHMVPTLVESFVLTVGIIFVSFLLVFRSGIARVMAMIPSF
                     FAILVMFLFMRSIGVFLNVATILIATTVLGTSENDQIHFFYHFQEGKKDNASTEAALR
                     HTLLISGKAIFFATLINAGGFLAFAFSTLPPIRQFGILSSVAFVLSMIADFTALPASL
                     WLLYREKPDSLKSSDGK"
     gene            complement(1951..2877)
                     /gene="rocF"
                     /locus_tag="PKL17_20515"
     CDS             complement(1951..2877)
                     /gene="rocF"
                     /locus_tag="PKL17_20515"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNK47177.1"
                     /translation="MQKRPNIVEIIGAPCDLGCNQRGANVGPAMVRVAGLADRLEELS
                     IRAFDGGDVPVPLRERLGRQTPENRDLYVSTIGQICDELFARVQASLRAGRFPLTLGG
                     DHSIAIGSIKGAADYCYQQGGQLGLLWVDAHADMNTPATSLSGNIHGMPLSTVLGLGF
                     ERLVHVGGIGPRVRPENVALVGIRDLDRDERTFVRETGIRYYTMKDIDQRGMYAVLTD
                     ALDDLTRRCAALHVSFDLDAVDPVYAPGVSTPVRGGLTYREAHLLLEVVAERGLLRSM
                     DLVELNPLNDVGHQTTRLCIELALSALGKAIV"
     gene            3158..>4597
                     /locus_tag="PKL17_20520"
     CDS             3158..>4597
                     /locus_tag="PKL17_20520"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK47178.1"
                     /translation="MRKSLLLSMVSVSLLQACGGNDQIAVEALSDDGQEVSAQAISLI
                     QLRGTHLGLDSNDVVRQRSRHVDELGHEHVRFERSYRGLPVKGGDFVVHTQAREDETF
                     SAMIAAAIPVSQRPDLTPAQAHSAAKQTWDIERTAMAEPKLVVHALGEAKDIVLAYEV
                     VAQGVQADGTPSVMHTFVDASSGRHLTSYDEIETGAATGSGRGINNGSVSLNTNLNGS
                     AYELVDTTHGNSPTINLANKTSGGSVFSQSANSWGDGTTANAASAGVDAHYGATKTWD
                     YYKTVHGRNGIANDGKGAQSRVHYSTKYNNAYWSDSCFCMTYGDGDGTQLRPLVALDV
                     AGHEMSHGVTSRTANLVYSGESGGLNEATSDIFGTAVEFYAGNSSDPGDYLIGEKIMI
                     GGKGYLRNMMDPTLDGASVDNYSKYKTSLDVHYSSGIANNFFYLLSEGGKNRTSGKTV
                     TGIGRAKAEKIWYRALTVYMTSSTNYKAAR"
BASE COUNT          935 a         1515 c         1276 g          871 t
ORIGIN      
        1 gcattccgca agtttgccac cggaaccaag ctattcagtc ggcaagggct cgtcggtgac
       61 cactttattt cgctcgcgtt ggtgctttcg atcaaaaaca ccgacgagcg acaggaaacg
      121 ctagggaaca tcgaaaaagc catcgccgac atcgaaaaaa gcccagcgcc cctgacccgc
      181 ctgcgcaaag tcggccagcc gatcgtcaac gcctacctcg acaacgacac ccgccaagcc
      241 gggctgcgct acttcccgct gtttttcctg ttcgtcgtcg tgctcaacgt cggactgtat
      301 cgatcatggc gagcactgct ggcattcatc gtcacgctgg ccatctgtgc cgcgttcacc
      361 gtcggttttg tcggtctgac acacggcgtc ttcacgattg tgtcgtcgct ggttcccatg
      421 acgatcttga tcgtctgcac cgcaacactg gtctatctgc actcacgcta tgtcgataga
      481 cccgaagacc gcagcgtcga agaccaccag atttttgcgc tctgtaacaa gtttctcgcc
      541 tgcaccgcat caattgtcgc gtcggcggtt ggttttgccg cactcgcggt gtccaaaatc
      601 cgtccgattc gcgaaatggg aatctgggtc gccgtcggcc ttttgatcac ctgggtgacg
      661 gtctttacgc tgtaccccgc cctggtgcga atcttgcgct gtccgacggg gcaggaacgg
      721 caaatcgccg gggcttggtt tactcgcctg gtcggtcacc tgccggtgtt ttcgtatcgc
      781 tatcgctacc tgctggtgtt cggatcgctt gtgctgtgtg ccatcggcgg catcgcggtg
      841 ttcggtcttc ccggcgtcgt caagccgatg aagctggaga ccgccgccat cgaatacatc
      901 ccgcacgaat ccgagatgta caaaagcacc aaagagatgg aaaagctcgt cggcggactg
      961 tcgattacca atgtctggct gcaaggcaag cccggcatgg tgtcaaacgc caaggtgctg
     1021 cgggggctga tggatttcca gaagcggctc gaccaggaac cgcagattgg ctcgacgcag
     1081 agcgtggtga cgcttttgtc gatgctgcgc tatctgtcga ccgataatga tgtactgccg
     1141 accgacgacg aaggtcttga agcgctgacc gatacgctcg aaacccagct tgaccgcgaa
     1201 cctttgcttc agagcttcgt cgacaaaggc aacgtctcgc agacccactt tgcggtggtc
     1261 agccggaccc aagatcacga cgaatttcag cagctcgcgc gggttgtcga aaaccactgg
     1321 aaggcatcgg tcgccgcgca cccggcactc aacgactttg cgatgcgtcc ggtcgggctc
     1381 gcgccgctgc aagccaagat ttcgcatcac atggtgccga cgctggttga gagcttcgtg
     1441 ctcaccgtcg gcatcatctt cgtgtcgttt ctgctcgtct tccgcagcgg catcgcccgc
     1501 gtgatggcca tgatcccgtc gttcttcgcc atcttggtga tgttcctgtt tatgcgctcg
     1561 attggcgttt tcctaaacgt cgcgaccatc ctgattgcga cgacggtgct cggcacgagc
     1621 gaaaacgacc agatccactt tttctaccac ttccaagaag gcaaaaagga caacgcttcg
     1681 acggaagctg cgctgcgcca cacgctgctc atttccggca aagccatctt ttttgcgacg
     1741 ctcatcaacg cgggcggctt tttggcgttt gctttttcga cgctgccgcc gattcgtcag
     1801 tttggcatcc tgtcgtcggt tgcattcgtg ctgtcgatga ttgccgactt caccgccctg
     1861 cccgccagtc tgtggctgct ctatcgcgaa aagcccgatt cgctgaagtc gtccgacggc
     1921 aaataagccc gccctgcccg ccttttgctt ttacacaatc gctttgccaa gtgccgacag
     1981 cgccagctcg atgcacagcc gcgtcgtctg atgcccgaca tcgttgagcg gatttagctc
     2041 gaccagatcc atcgatcgca aaagcccccg ctcagccacc acttcgagca aaagatgcgc
     2101 ctcgcgatag gtcagaccgc cacgcaccgg cgtgctgacc cccggcgcat agaccggatc
     2161 gaccgcatcc agatcaaacg acacatgcag cgccgcgcag cgccgggtca ggtcgtcgag
     2221 cgcatcggtc agcaccgcat acatgccgcg ctgatcgatg tctttcattg tgtaatagcg
     2281 aatgccagtc tcgcgcacga acgtccgctc gtcgcggtcc aggtcgcgaa tccccaccag
     2341 cgccacattt tcaggccgca cccgcggacc gatgccaccg acgtgtacca gccgttcaaa
     2401 ccccaggccc agcaccgtgg acagcggcat gccgtgaatg ttccccgaca gcgacgtggc
     2461 cggcgtgttc atgtcggcat gggcatcgac ccacaaaagc ccaagctgcc cgccctgctg
     2521 atagcagtag tccgccgcgc ccttgatcga tccaatcgca atcgagtggt ccccgccgag
     2581 cgtcagcgga aaccgtccag cccgcagcga cgcctgcaca cgcgcaaaca gctcatcgca
     2641 aatctgcccg atcgtcgaaa catacaggtc gcggttttcc ggcgtctgcc gtcccagccg
     2701 ctcgcgcagt ggcaccggca cgtccccgcc atcgaacgca cgaatcgaca gctcttccag
     2761 ccggtcggcg agccccgcca cccgaaccat ggccggcccg acgttggcac ctcgctggtt
     2821 acagcccaag tcacacggtg ccccgatgat ttcgacgatg ttcggacgtt tctgcatggc
     2881 tttaccccac agcagtcagt ctgccttgta gcgcagttgt agcgaaatcc gctttgcaaa
     2941 gaaaagccca cctacgccca tgcgcatgct ggggtgccgg ccacccaagt ggggcctgca
     3001 ctcccatgcg atccccatcc cccggaacgc catcgtgctt acgttgacgt cgggaagcca
     3061 agatcccaag cctcaccagg aagcgctgag aagcttggca cgcaattcgc aatagcaaaa
     3121 accaacaacg aatcccccac aaggaaaggg aggtcccatg cgtaagtctc ttcttctgtc
     3181 tatggtgtcg gtgtcgcttc tgcaagcttg tggcggcaat gatcagattg cggtcgaagc
     3241 cctaagcgat gatggtcaag aagtcagcgc gcaggcgatt tcccttatcc aactgcgtgg
     3301 cacccacttg ggtctcgact caaatgatgt ggtgcggcag cgcagccgtc atgtcgacga
     3361 gcttggccac gaacacgtcc gcttcgagcg cagctaccgc ggcctgccgg tcaaaggcgg
     3421 cgactttgtc gtccacaccc aggcgcgcga agatgagacc ttttcggcga tgattgcggc
     3481 ggcgattccg gtcagccagc gccccgatct gaccccagcg caagcgcaca gcgccgccaa
     3541 gcagacctgg gacatcgagc ggaccgcgat ggccgaaccg aagctggttg tccacgcgct
     3601 cggcgaagcc aaagacatcg tgctcgccta cgaagtcgtc gcgcagggcg tccaggccga
     3661 cggcaccccg agcgtgatgc acaccttcgt cgatgcaagc agcggtcgcc acctgacctc
     3721 gtatgacgaa atcgaaaccg gcgccgccac cgggtcgggc cgcggcatca acaacggcag
     3781 cgtgtcgctc aacaccaact tgaacggctc ggcctacgag ctggtcgaca ccacgcacgg
     3841 caactcgccg accatcaacc tcgccaacaa gacgtcgggc gggtcggtgt tcagccagtc
     3901 ggccaacagc tggggcgatg gaaccaccgc caacgccgcc agcgccggcg tcgatgccca
     3961 ctacggcgca accaagacct gggattacta caagaccgtc cacggcagaa acggcatcgc
     4021 caacgacggc aaaggcgcgc agtcacgggt ccactacagc accaagtaca acaacgccta
     4081 ctggtctgac tcgtgcttct gcatgaccta cggcgacggc gatggcaccc agctccggcc
     4141 tctggtcgcg ctggatgtcg ccggccacga aatgagccac ggcgtcacct cgcgcacggc
     4201 caacctggtc tactcgggtg aatcgggcgg tctgaacgaa gcgacgagcg acatcttcgg
     4261 caccgcggtt gagttctacg ccggcaacag ctcggaccct ggcgactacc tgatcggcga
     4321 aaagatcatg attggcggca agggctacct gcgcaacatg atggacccga cgctggacgg
     4381 cgcttcggtc gacaactaca gcaagtacaa gaccagcctc gatgtgcact actcatcggg
     4441 catcgccaac aacttcttct accttctgtc ggaaggcggc aaaaaccgca cctcgggcaa
     4501 gaccgtcacg ggcattggcc gcgccaaggc ggaaaagata tggtaccgcg cgctcacggt
     4561 ctacatgacg tcgagcacca actacaaggc ggcgcgc
//
LOCUS       DAORGN010000294         4588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_9876_length_4588_cov_4.790867, whole genome shotgun sequence.
ACCESSION   DAORGN010000294 DAORGN010000000
VERSION     DAORGN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9876_length_4588_cov_4.790867"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKL17_20525"
     CDS             complement(<1..530)
                     /locus_tag="PKL17_20525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNK47179.1"
                     /translation="MKLHELDATALLAALAKGETSSVEIVQSLIERRKSIGEKVSAIV
                     LPLDDSARKAAEAADAARKRGDTLLPLHGLPVSIKDNFEVIGTPATLGVVRRRNHHSQ
                     QDAVAVAALKHAGAVVLGKTNVPQLLLVQESDNAIYGTTKNPWNRDRSPGGSSGGEGA
                     AIATGQSPLGLGSDIGG"
     gene            630..1961
                     /locus_tag="PKL17_20530"
     CDS             630..1961
                     /locus_tag="PKL17_20530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK47180.1"
                     /translation="MTSVKLVSEQPFGEGGLTSRLHELPNGLKVLLLRDPSAPVFAYQ
                     TWFSVGSRHEREGKTGIAHLFEHLMFNETQNLPHGEFDRRFELAGGNTNAATWVDWTY
                     YQDDLPSSELELAVTLEAERMQNLLVRERQVETERGVVISERQMRVDDDVDGFMSEEL
                     FRRAYTVHPYMWPTIGWMQDIKNLNLDDCVGFYRTYYAPNNATVVVVGDIDEQKTLDL
                     IVKHYGGIAAQPIPPHVQVTEPPQNEPRLARFKKPVVTDRILFGYKAPALHDPDHLRL
                     ELLSELLIGGPSSPIYRDLVIEREIFSSLGGSVTPFRDPGLWEIAASLHRGHSADEGL
                     SQFDAHIAKVVAQGPSQAEVDRARARMLTSFYLSLKTAHGKASSLGESQTTLGDYREL
                     WRVPDILRSISVADLHAVAKRYLDPAQRTVVIAEPSGEVSEEDDGDEEGEA"
     gene            1965..3275
                     /locus_tag="PKL17_20535"
     CDS             1965..3275
                     /locus_tag="PKL17_20535"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNK47181.1"
                     /translation="MAIILEENHDLPLCKLQITQRVGSTSDQTAAGTAAYGGPALSGL
                     CNFASELERRGAGGRTRAEIDEQLDSLGAGLQILCWHDQVLYEVMSLREHFERAVEIL
                     ADVVLRPDFPMDEGERLRRELLANLDELRDDDSGLLHRFFGRELYGDTPYGRPVAGTE
                     ESTMALSLPAAKAWHSHHTVRGNLVVGAAGAISGSEAESLMQRYFSTLPDGPRRDEPI
                     PSPRARKGRKVLIVDKPERTQSQILIGQLAPHWKDPSWLPLHVSTMAFGGTFTARLMD
                     EVRAKRGLSYGASARLGNGRGIRSLYAHVFPSAEQTAETIDLVLRLYEEWAEKGLRPG
                     ELDFVKSYLQKSHAFTVQTADDRLNLRTRLQVCDMPEDDEKTYPDRVARVSEADIQRA
                     MASFLHPDDLLITLVATADTVLPSLQKLPGLRNAEFEVVPHDSF"
     gene            3285..4445
                     /gene="queG"
                     /locus_tag="PKL17_20540"
     CDS             3285..4445
                     /gene="queG"
                     /locus_tag="PKL17_20540"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNK47182.1"
                     /translation="MSDREEEAAATARVRAACAEVGLSIVGITQALALPDHELYLAWL
                     AAGYAGELAYLHRDAADRADVRNLLPQARSVLTVALSYHFPFDEEVKPGHGKIARYAR
                     GTDYHLVLKARLQDLVSRLASGFSDPLLARVCVDTAPLLERSLAAQSGLGFVGKNTLL
                     ISPGIGSYTVLGEVLLSVPLSPAAPQSPRCGECRLCLDACPTEALRAPFQLDARRCIS
                     YLTIENSGTVPVALRKQVGDWIFGCDICQSVCPYNARAPLGDAELAPKAKLCSPLLSQ
                     LLSTGAAQYRQLVKRTALRRINRNSLLRNVAVALGNVGTPADLPVIAAALLRESSLVR
                     IHLSWAAAEIALRHPDTRALVVDMLQTARSVESDAEVALALDADIKRLPPQA"
     gene            complement(4490..>4588)
                     /locus_tag="PKL17_20545"
     CDS             complement(4490..>4588)
                     /locus_tag="PKL17_20545"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007472189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HNK47183.1"
                     /translation="AEEYHQQYLAKNPDGYCGHGGTGVACPVGLKR"
BASE COUNT          820 a         1328 c         1522 g          918 t
ORIGIN      
        1 ccgccgatgt ccgaacccag tccaagcggc gactggccgg tcgcgatcgc tgcgccttca
       61 ccaccggatg aaccacctgg gctgcgatcg cgattccacg ggtttttggt ggtgccgtaa
      121 atcgcgttgt cgctttcttg cacaagcaga agctgcggca cgttggtctt gcccaacacc
      181 acggcaccag cgtgcttgag cgcggccacg gccacggcgt cttgctggct gtgatggttg
      241 cggcgtctga ccacgccgag cgtcgccggg gtgccgatga cttcgaagtt gtctttgatg
      301 ctgaccggca gaccatgcag cggcaaaagt gtgtcgccac gcttgcgtgc ggcgtcggcg
      361 gcttccgcgg ctttgcgggc gctgtcgtcc agcggcagga cgatggccga cactttttcg
      421 ccaatcgact tgcgtcgttc aatcagtgat tggacgatct cgacggaaga agtctctccc
      481 ttggcgagcg ccgcaagcag tgcagtggca tctagctcat gtagcttcat gacaaaggca
      541 gcatatccta gacaagctcg cgtgcttctt ctttgacagg cggctcgacg agcgatacac
      601 ccattcgacg gacaaaggaa cttgttctga tgacttctgt gaagctggtt tccgagcagc
      661 catttggtga aggcggcctg acctctcgtc tgcatgagct accaaacggg ctgaaagttc
      721 tgctgctgcg cgatccgtcg gcgccagtgt ttgcctatca gacctggttt tcggtcggtt
      781 ctcggcatga gcgagagggc aagaccggca tcgcgcacct gtttgaacac ctgatgttta
      841 acgaaaccca gaacttgccg cacggcgagt tcgaccgtcg ttttgagctg gcgggtggca
      901 acaccaacgc ggccacttgg gtggactgga cgtattatca ggacgacttg ccgtcgagcg
      961 aactagagct ggcggtgacg ctggaagccg agcggatgca gaacctgctc gtgcgtgagc
     1021 gccaagtcga gaccgagcgc ggggtggtga tctcggaacg gcagatgcgg gtcgatgatg
     1081 acgtcgacgg cttcatgagc gaggagctgt ttcggcgggc gtacacggtt catccgtaca
     1141 tgtggccgac gatcggctgg atgcaggaca tcaaaaacct gaatctggat gactgcgtcg
     1201 gcttttatcg gacgtactac gcgccgaaca atgcgacggt ggtggtggtc ggcgacatcg
     1261 acgaacaaaa aacgctcgat ttgattgtga agcactacgg tggcatcgcg gctcagccga
     1321 ttcctccgca tgtgcaggtc acggaaccgc cacagaatga accgaggctg gcccggttca
     1381 aaaaaccggt ggtgacggat cggattctgt tcggctacaa ggccccggcg ctgcacgatc
     1441 cagatcacct gcggcttgaa ctgttgagcg agcttttgat tggtgggcca tcgtcgccca
     1501 tctaccgcga cctggtcatc gagcgcgaaa tcttttcgtc gctgggtggt tcggtgacgc
     1561 catttcgtga tccgggcttg tgggaaatcg cggcatcgct gcatcgggga cacagcgcgg
     1621 acgaagggct ttcgcagttt gacgcacaca tcgcgaaggt ggtcgcgcag ggtccgtcgc
     1681 aagccgaagt ggatcgggcg cgggcgcgga tgctgacgag cttttatttg tcgctcaaga
     1741 ccgcgcacgg caaggcgtca tcgcttgggg agtcgcagac aacgcttggc gactaccgcg
     1801 agttgtggcg ggtgccggac attttgcgct cgatttctgt cgctgatctg catgcggtgg
     1861 ccaagcgcta cctcgatccg gcgcagcgca cggtggtcat tgccgagcca agcggcgaag
     1921 tcagcgagga agacgacggc gacgaagagg gagaggcgta ggcgatggcg attatcctgg
     1981 aagagaacca cgacttgccg ctgtgcaagc ttcagatcac ccagcgggtc ggctcgacct
     2041 ctgatcagac ggcggcgggg acggctgcat acgggggtcc ggcgctgtcg ggtctgtgca
     2101 atttcgcaag tgagcttgag cgtcgcggcg ctgggggtcg aacgcgggcg gaaatcgacg
     2161 aacagctcga ctcgctcggg gcggggctgc agattctgtg ctggcacgat caagtgctgt
     2221 acgaagtgat gtcgctgcgc gagcacttcg agcgggcggt cgaaatcctg gccgatgtgg
     2281 tgctgcgtcc ggacttcccg atggacgaag gggagcggct gcggcgcgag ctgttggcca
     2341 atctggatga gctgcgcgac gatgacagtg gacttctgca tcgtttcttt ggtcgcgagc
     2401 tgtacgggga tacgccctac ggtcgtccgg tcgcgggcac cgaagaaagc acgatggcgc
     2461 tgtcgcttcc cgccgccaag gcttggcaca gtcatcacac agtgcgcggc aacttggtgg
     2521 ttggggccgc cggagcgatt tcagggtccg aagcagaatc gctgatgcag cgctactttt
     2581 cgacgctgcc ggacggacca cgccgcgatg aaccgattcc ctcgccgcgc gcgcgcaagg
     2641 gtcgcaaagt tctgattgtc gacaaaccag agcgcaccca gtcgcagatt ctgattggcc
     2701 agctcgcgcc gcactggaaa gacccgtcgt ggctgcccct gcatgtgtcg acgatggcgt
     2761 ttggcggcac gtttacggcg cggctgatgg acgaagtgcg cgccaagcgc ggtctgtcgt
     2821 atggagcgtc ggcgcggctt ggcaacgggc gcggcatccg ttcgctgtat gcacacgtct
     2881 tcccatcggc agaacagacc gccgaaacga ttgatctggt gctgcggctg tacgaagagt
     2941 gggcggaaaa agggctccgt ccgggcgagc ttgattttgt gaaaagctac ctgcaaaaaa
     3001 gccacgcctt cacggtgcag actgctgacg accggctgaa cctgcggacg cgactgcaag
     3061 tgtgtgacat gccggaagac gacgaaaaga cctatcccga tcgggtcgcg cgcgtcagtg
     3121 aagccgacat ccagcgcgcg atggcgtcct ttctgcatcc cgacgacctg ctgatcacgc
     3181 tggtggcgac ggctgacacg gtgctgccga gcctgcaaaa gctgcccgga ctgcgcaacg
     3241 ctgagtttga agtcgttccg cacgattcgt tctagcgata agccatgtcg gaccgggaag
     3301 aggaagccgc agcgacagcg cgggtgcgtg cagcgtgtgc ggaggtcggt ctgtcgattg
     3361 ttggaatcac gcaggctttg gcactgcccg atcacgagct gtacttggcc tggctcgcgg
     3421 cgggctatgc gggagagcta gcgtatctgc accgcgacgc ggcggaccgg gccgacgtgc
     3481 gcaacctgct gccgcaggca cgatcggtgc tgacggtcgc tttgtcgtac cattttccgt
     3541 ttgacgaaga agtcaaaccg ggtcacggca agatcgcgcg ctatgcccgt ggaaccgact
     3601 atcacttggt gctcaaagcg cggttgcaag accttgtgtc tcgcctggcg tccggttttt
     3661 ctgacccgct gctggcgcga gtctgtgtcg atacagcgcc gcttctggag cgatcgttgg
     3721 cagcgcaatc gggacttggt tttgtcggca aaaacacgct gctgataagc cccggcattg
     3781 gcagttacac ggtgcttggg gaagtgctgc tgagtgttcc gctttcgccc gctgcgccgc
     3841 agagtccgcg ctgtggcgaa tgccgtctgt gtctggatgc ctgtcccacc gaagcgctgc
     3901 gggcaccgtt tcagcttgat gcgcggcgct gcatttccta tctgacgatc gaaaacagcg
     3961 ggacggttcc cgtcgcgctg cgcaagcaag ttggggactg gatctttggc tgcgacatct
     4021 gccaatcggt ctgtccgtac aacgcccgcg ccccgcttgg ggatgcggag ctggccccca
     4081 aagcgaagct ctgttcgccg cttttgtcgc agctattgtc caccggggcg gctcaatatc
     4141 ggcagcttgt gaagcgcacg gcgctgcgcc gaatcaaccg caactcgctg ctccgcaatg
     4201 tcgcagtggc cctgggcaac gtcggcacac cggctgactt gcctgtgatt gccgcggcgc
     4261 tgctgcgcga gtcctcgctt gtgcgcatcc atctgtcgtg ggctgccgcg gaaattgcgc
     4321 tgcgccaccc ggacacgcgg gcgctggttg tggacatgtt gcagacggcg cggtcggtcg
     4381 aatcggatgc cgaggtcgcg cttgcactgg atgcggacat aaaacggctg ccgccgcagg
     4441 cataaagccc ggcccggccc gaccagccgc tttcgtcgcc ctgttcgcgc tagcgtttga
     4501 gtccgaccgg gcaggcgacg ccggttccgc cgtgtccaca gtagccgtcg ggatttttgg
     4561 ccaggtactg ctggtggtac tcctcggc
//
LOCUS       DAORGN010000295         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10196_length_4500_cov_4.625647, whole genome shotgun sequence.
ACCESSION   DAORGN010000295 DAORGN010000000
VERSION     DAORGN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10196_length_4500_cov_4.625647"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKL17_20550"
     CDS             complement(<1..200)
                     /locus_tag="PKL17_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47184.1"
                     /translation="MKKIPVLLSLLFASTGLFGCTETPDPCAGYSLACIGVTVEGGPP
                     STYQLLVRVLDGYGSVTPVTPR"
     gene            complement(197..1003)
                     /locus_tag="PKL17_20555"
     CDS             complement(197..1003)
                     /locus_tag="PKL17_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47185.1"
                     /translation="MLLFVQSLLGSPVVAHAADQQQAAPPTDSVKQQVRQLIREAGQM
                     MERGDRRGAAARLEQARALWNEPSIDYNLGVVYGELQQPQEAAQALERFLRNADRAMV
                     LSERLEDAKKRLAAYERSLSRLSVTVTMPSGSSEPNLFLDGSLRSKLPDGNTPPPGYL
                     FATAGSHQVRVASSGLRDYSVSVDLKAGELRKLDGILLPQSGDAALLSYSTPPQNAGS
                     DTPPIYKRWWFWAAVGGGVAVIAGISGAAAAGAFHRVAPGSDLDPIDVSR"
     gene            1195..2097
                     /locus_tag="PKL17_20560"
     CDS             1195..2097
                     /locus_tag="PKL17_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47186.1"
                     /translation="MQQPPEWPKLKSSLEAKGAMDYRTFEQKLLDTIFTTDVPLNPAT
                     IAFLYKLTVQQASDLLQQAAVNGLLNIESDEEGNLLYTYPNRVRLPRDGKIDPRREAA
                     AKAASTALVPTGPASVVKATSTEPAPLPDSIDLLEASAPRDDAKAAASAPPPLTSVSG
                     TAPRESPGGMTRCRFCNEVILVGAKKCKHCHEYLDDALREIQNLKQPGGMHHPPPQTA
                     LAPQPTPQPVQIQQWTNTNATQAALLSFFVPGLGQMCSGRVPAGLLWMMFTCLGYVCF
                     IVPGIVLHILCVINAARPQQPATQ"
     gene            complement(2087..3217)
                     /gene="sctQ"
                     /locus_tag="PKL17_20565"
     CDS             complement(2087..3217)
                     /gene="sctQ"
                     /locus_tag="PKL17_20565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HNK47187.1"
                     /translation="MSLPSRPRRVRPFPLAELPRLLRVQVELSRLLWQHILAVIDAAA
                     SPLGGLHDLANRLGGMPQIRTVEPYLVPSDRLAAQISGGQVVELTVGRSEREVILVVE
                     SALCQRLSIETPDDLTKLLRSALLAAEVRVRAINSDETASVLDVTSHPQLIALDATVT
                     TTQAQGWAKILCRPDIRHWAPPAPSIAAAESRWQRRERLRMLQVPLTIEAGYGFLPAA
                     DVVALRPDDIVVLDHFGPRPVTGGPVALRLQGGVFPAFLDGAGVTIMAPFHLRAESMA
                     DTQNESDQHDAPATGTEPSTQPSSPSEHLLRELPVQITCEIGRVTLTAREILELRPGA
                     VVPVGRPLAGPVDLTAGGRVIARGELVDVEGELGVRVTEVTE"
     gene            complement(3214..3762)
                     /locus_tag="PKL17_20570"
     CDS             complement(3214..3762)
                     /locus_tag="PKL17_20570"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNK47188.1"
                     /translation="MTATEAGRTDEAVQMSAKWSRRERIAIQIATVFRIGEVAPWPGH
                     YATLFTVPFAIALHKTGPWPHAIATLLLFAIGTWAAEEYSRLTGKHDHRSIVIDEVVG
                     YLVTVWLAPWHFLSFFLGYFLFRVLDGLKPGPLRLLDRHVKGGFGVMLDDVAAGAVGA
                     LILHWLVPWTAPTFDAMLQKLW"
     gene            3856..4257
                     /locus_tag="PKL17_20575"
     CDS             3856..4257
                     /locus_tag="PKL17_20575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNK47189.1"
                     /translation="MSQDRRQQLRLPVEICVQQYLSDGVYLGLTRDLSETGLYLTAPR
                     LRDDMTLLGAPIHLEFALPGTGDRIFARGQVCYESLDGDLKGLGIRLQDMADRHKQSL
                     HRYVESVRRARLHNMLWQMNQPTMRLPGVVR"
     gene            complement(4254..>4500)
                     /locus_tag="PKL17_20580"
     CDS             complement(4254..>4500)
                     /locus_tag="PKL17_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009680762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNK47190.1"
                     /translation="KHARARRAWVQLGHDGSDLKLSVYDDGQAPQQPLGEGLGLLGIR
                     ERAKLLGGRAEITVDANRSFRVEVTLPLDEAAAPTVD"
BASE COUNT          909 a         1395 c         1385 g          811 t
ORIGIN      
        1 ttgcgcggcg taaccggcgt gacactgcca tagccgtcga gtacccgcac gagcagttgg
       61 tacgtgcttg gcgggccgcc ttcgacggtc acaccgatgc aagcgagcga ataaccagcg
      121 cacggatcgg gcgtctcggt gcatccgaaa agacccgtgc tggcaaaaag cagcgagagc
      181 aaaacaggga tcttcttcat cgcgacacgt cgatggggtc aaggtccgaa ccgggagcga
      241 ctcgatggaa tgcaccagcc gccgcagccc cggagatgcc agcgatgacg gccacgccac
      301 cgccgacagc ggcccaaaac caccagcgct tgtaaatggg cggcgtgtcc gagccggcgt
      361 tttgtggtgg cgtcgaatag ctgagcagcg ccgcgtcacc gctttggggg agcaatattc
      421 cgtcgagttt gcgcagctca cccgccttta ggtcgaccga caccgaatag tcccgcagtc
      481 ccgacgacgc cacgcgaacc tgatgcgaac cggcggtggc aaacagatat cccggcggtg
      541 gcgtatttcc gtcgggcagc ttgctgcgca gcgatccatc caaaaacagg ttcggttcag
      601 aagatccgct tggcatcgtc accgtgacgc ttagccgact gagggatcgc tcgtaagcgg
      661 ccagtcgttt tttggcgtcc tcgagccgtt ccgaaagaac catcgcgcga tcggcattgc
      721 gcaaaaaacg ctcgagcgct tgggctgctt cctgcggctg ttgcagctct ccgtagacaa
      781 cgccgaggtt gtagtcgatc gacggttcat tccaaagcgc ccgcgcctgt tcgagccgag
      841 ccgcagcgcc ccgacggtca ccgcgttcca tcatctgacc tgcttcgcga atcagctgac
      901 gaacttgttg ttttacggaa tcagtcggcg gggctgcttg ttgctgatct gcggcgtgtg
      961 ccacgaccgg gctacccagc agcgattgga caaaaagcag catgcctgcg acgctggttc
     1021 gtcgaaaaac gtcttgcgga agcgacatga ggggcggcac gttaccacgg aaaaaacggc
     1081 cacagtcaaa cagcgcgtgt tcgtagttag actggcggca ttgcgatccg gacgccgcgg
     1141 atacatcaaa agaaaacgag gccccgcgca tgctgtataa agtcggacct gcaattgcag
     1201 cagccaccgg aatggcccaa gctaaaatcc tctctggagg ccaaaggcgc gatggactat
     1261 cgcacgttcg agcagaagct gctggacacg atcttcacga cggatgtccc gctgaacccc
     1321 gccacgatcg ccttcttata caagctgacg gtgcagcaag cttccgatct gcttcagcag
     1381 gcggcggtga acgggctgct caacattgag agcgacgagg agggcaacct cctttacacc
     1441 tatccgaacc gcgtgcgctt gccacgcgat ggcaaaatcg atccacgccg tgaagccgca
     1501 gccaaagcgg cgtcgacggc gctggtgcca actggaccgg cttcggtggt caaagccacg
     1561 tctactgaac cggcgccatt gcccgacagc atcgatcttc tggaagcttc agccccacgc
     1621 gacgacgcaa aagccgcagc cagtgcgcca ccgcctttga cctctgtgtc tgggacggcg
     1681 ccacgggaat cgccaggtgg gatgactcgc tgccgcttct gtaacgaagt catcctcgtt
     1741 ggtgcgaaga agtgcaagca ctgccacgaa tatctggatg acgcgctgcg cgagattcag
     1801 aacttgaagc agccgggcgg gatgcatcac ccaccgccgc agaccgcgct ggcaccgcag
     1861 ccgacgcctc agccggtgca gattcagcag tggacgaaca ccaacgcgac gcaggccgcg
     1921 ctgctgagct tctttgttcc ggggcttggc cagatgtgca gcggacgggt tccggcgggg
     1981 cttttgtgga tgatgtttac gtgcctcggc tacgtttgtt tcatcgtgcc ggggatcgtg
     2041 ctgcacattc tctgcgtgat caacgcggcc cgtccgcagc agccggctac tcagtaactt
     2101 ccgtcacgcg gacgcccagc tcgccttcga catccacgag ttcaccgcgc gctatcacgc
     2161 ggcccccggc agtcaaatca acaggtccag ccagcggtcg tccgacggga accactgcgc
     2221 caggccgcaa ttccaggatc tcgcgagcgg tcagcgtgac gcggccaatc tcgcaggtga
     2281 tctgaacggg tagctcgcgc agcaggtgtt cactcgggga tgacggctgg gtgcttggct
     2341 cggttcccgt cgcgggcgcg tcatgttgat cggattcgtt ctgggtatca gccatagact
     2401 ctgctcgcag gtgaaatggc gccataatcg tgacgccggc tccgtcgaga aatgctggaa
     2461 atacgccacc ttggagccgc agcgccaccg gaccgcccgt caccggacgc ggcccaaagt
     2521 gatcgagcac cacgatgtcg tcgggccgca gcgccaccac atcagccgcc ggcaaaaaac
     2581 cgtagccagc ctcgatggtc agcggcactt gcaacatgcg caggcgttcg cgtcgctgcc
     2641 aacgtgactc cgccgccgca atcgaaggtg ccggcggggc ccaatgccgg atgtcgggtc
     2701 gacagaggat tttcgcccag ccctgggctt gtgttgttgt caccgtcgca tccagcgcga
     2761 tgagctgtgg atggctggtc acatcaagga cgctggccgt ttcgtcgctg ttgatggcgc
     2821 gcacccgcac ttcggcggcc aaaagggcgc tgcgcaacag cttggtcagg tcgtctggcg
     2881 tctcgatgga caagcgctgg catagcgctg actcgacgac aagaatcact tcgcgttccg
     2941 atcggccaac ggtcagctcg acaacctgtc cgccgctgat ttgcgcggcc aagcgatcgc
     3001 tcgggacaag gtatggttcg acggtgcgga tctggggcat gccacccagc cgattcgcca
     3061 gatcatgcag cccgccaagt ggtgacgccg ccgcatcgat cacggccaag atgtgctgcc
     3121 aaagcagccg agacagctca acctgtacgc gcaaaagccg cggcagctcc gcgagtggga
     3181 acggacggac tcttcggggt cgggaaggca agctcaccac agcttttgta gcatcgcatc
     3241 gaatgtcggc gcagtccacg gtacgagcca gtggagaatc agcgcgccca cagcccctgc
     3301 cgcgacatcg tcgagcatca cgccgaaacc gcctttgacg tgacggtcca aaagccgcag
     3361 cggccctggc ttcagcccat cgagcacgcg aaacagaaag tacccgagga agaagctcaa
     3421 aaagtgccac ggcgcgagcc agaccgtcac caagtagccg acgacttcat caatgacgat
     3481 cgatcgatgg tcgtgtttgc cggtcaggcg cgagtactct tccgccgccc atgtcccgat
     3541 cgcgaacagc aacagcgtgg cgatcgcatg aggccacggt cccgttttgt gcaacgcgat
     3601 ggcaaacgga actgtaaaaa gtgtggcgta atgccccggc caaggcgcga cttcgccaat
     3661 ccgaaagacg gtggctatct ggatggcaat tcgctcgcgg cgggaccact tggccgacat
     3721 ctgcaccgct tcatcggtac ggccagcctc cgtcgcggtc aacgaaattc acaaaataaa
     3781 tttgcggtcg atcgcacctt gtcctacatc atgcgacata agcgccttgc gaccacaagg
     3841 cgaggagatg tgaccatgag ccaagatcgt cgtcagcagc tgcgtttgcc ggtggaaatc
     3901 tgcgtccagc agtacttgtc cgatggcgtg tatctggggc tgacccgcga cctttcggaa
     3961 acggggctgt atctgacggc gccgcggctg cgcgatgaca tgacgctgct cggggcgccg
     4021 atccatctgg agtttgcgct gccggggacg ggcgatcgca tctttgcgcg cggtcaggtc
     4081 tgctatgagt cgctcgacgg tgatctgaag ggactgggga ttcggctgca agacatggct
     4141 gatcggcaca agcaaagcct gcatcgctat gtcgaatcgg tgcgtcgggc ccggctgcac
     4201 aacatgctgt ggcagatgaa tcagccgacg atgcgtttgc ctggcgtggt tcgctaatcg
     4261 acggtggggg ccgcagcctc atcaagaggc agtgtgacct cgacacgaaa gctgcggttt
     4321 gcgtcgacgg tgatttcggc gcgaccaccg agcagcttgg ctcgttcgcg tattcccaac
     4381 aggccaagtc cctcgccaag tggctgctgt ggggcctggc cgtcgtcgta gaccgatagc
     4441 tttaggtcgc ttccgtcgtg gccaagctga acccacgccc gacgggctct ggcgtgtttt
//
LOCUS       DAORGN010000296         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10213_length_4494_cov_4.792971, whole genome shotgun sequence.
ACCESSION   DAORGN010000296 DAORGN010000000
VERSION     DAORGN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_4494_cov_4.792971"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKL17_20585"
     CDS             complement(<1..322)
                     /locus_tag="PKL17_20585"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNK47191.1"
                     /translation="MSHHRPRFDSHALSHKLRNRSPARWQLDGFRESAVLVPLVVPQS
                     NPSGDPEVLFIVRTASLPTHAGQVAFPGGKRESTDPTLEHTALRESHEELGIAAERVT
                     LLGPL"
     gene            complement(319..1248)
                     /locus_tag="PKL17_20590"
     CDS             complement(319..1248)
                     /locus_tag="PKL17_20590"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNK47192.1"
                     /translation="MNQALDPTARARKNETPLRRPALHLAFCFGMTVALTGCPPPPVV
                     IRPYPAPNAAELLSDVRKSQAKVQSMKLYAKADVPDDGGGRVKLDVSIAVKRPAQLRL
                     TAESTLTGPLLTLATDGQRFQLLDAQNNRYLSSVVTPCSMARLLRVALPPTILADVLS
                     GGVPLLPPDSLTVQSDWDEKEGGREVIKLRDPEGRRQVLYLQPSEPGQAQSRVFDVVE
                     SELSDRDGKPFLRIRHQKFAPVQTDPMPADPVRLPKQSTIEDLTSRTEVKLRWKETEV
                     NPALDATLFYLPQPVGIPTDPDPCVPAAAAKSP"
     gene            1737..2837
                     /gene="serC"
                     /locus_tag="PKL17_20595"
     CDS             1737..2837
                     /gene="serC"
                     /locus_tag="PKL17_20595"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNK47193.1"
                     /translation="MTTHRIFNFSAGPAVLPEEVLLEARDNLLSLGSSGIGILECSHR
                     SKDFEAIIGEAEANLRKLLGIPSGYHVLFLSGGATTQFSMVPMNLLLPGTVGDYVLTG
                     EWSKKALLEAQRVGETHVAATTEAEQFRRIPKNEELHCSDRSAYVHTTSNNTIWGTEW
                     HHEPDAGGRPIVCDMSSDFLSRPVDVARYGLIFAGAQKNAGPAGVTVVIVRDDMLRLD
                     KAAQAKLPVMMNYRTFVDQKSLYNTPPVFPIYIVHLVLRWLVRFGGLAKMASHNQEKA
                     QFIYDVIDDASGFYRGHATRDSRSLMNITFRLPSEALEDKFAKEAKAAGMDGLKGHRS
                     VGGIRASVYNAFPIAGAQALANFMRDFAARNG"
     gene            2966..>4494
                     /gene="lepA"
                     /locus_tag="PKL17_20600"
     CDS             2966..>4494
                     /gene="lepA"
                     /locus_tag="PKL17_20600"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNK47194.1"
                     /translation="MYQNHIRNFSIIAHIDHGKSTLADRLLEACGALSDREKQDQFLD
                     KMELERERGITIKAQTVRLRYRAKDGKEYQLNLIDTPGHVDFHYEVSRSLAACEGALL
                     VVDASQGVQAQTLANVYVALDSNLEIVPVLNKIDLPSAEPERIRKQIEDVIGIDASMA
                     IPASAKDGRGIPEILEAVVEMVPPPKGDPTAPLQALMFDSWYDNYRGVVIQVRVKNGS
                     VKIGDKIQLMATGKPYEVLGLGVFNPHPIEIKELRCGEVGFIIANIKTIADTQIGDTI
                     THADRPCSEPLAGFKVVKPMVFAGIFPTDNAKYTDLRDALEKLHINDASFTYDPETSL
                     ALGFGFRCGFLGLLHMEIVQERLEREYDLDIITTAPTVEYRATTTSGETIIVDNPAAL
                     PDEGKLDYIEEPIISASIYTPSEYVGALITLCQERRGVQVGMTYNGEDRVVLKYELPL
                     AEVVFGFYDRLKSVSRGYASLDYELLEYRRADLIRLDLLVNGDRVDALSIICHRERSY
                     YR"
BASE COUNT          870 a         1348 c         1396 g          880 t
ORIGIN      
        1 cgagcggacc cagcaaggtg acgcgctctg cggcaatccc cagctcttcg tggctttcgc
       61 gcagcgcggt atgttccaac gtcgggtcgg tcgactcgcg ctttccaccg ggaaacgcga
      121 cctggcctgc gtgggttggt agcgatgcgg tgcggacgat gaacaggact tcgggatcgc
      181 cgctcgggtt gctttggggc acaaccagtg gcaccagcac cgccgattca cggaaaccat
      241 cgagctgcca gcgcgcaggg gatcggttgc gcagcttgtg cgacagggcg tgggaatcga
      301 agcggggacg gtgatggctc atgggctttt cgcagccgct gccgggacgc aagggtcggg
      361 gtcagttgga atgccgaccg gctgcggcaa atagaatagc gtggcatcga gcgcggggtt
      421 tacttccgtc tctttccagc gcagcttgac ctcggtacgg ctggttagat cctcgatggt
      481 ggactgcttg ggcaggcgca ccgggtccgc gggcatcggg tcggtttgga cgggcgcaaa
      541 cttctggtgg cggatgcgca aaaacggctt gccgtcgcga tccgacagct cgctttcgac
      601 cacatcgaaa acccggcttt gggcttgccc cggctcactt ggttgcaaat acagcacttg
      661 gcgacggcct tcgggatcgc gcagcttgat cacctcgcgt ccgccttctt tttcgtccca
      721 gtcggactgc acggtcagcg agtcgggtgg caaaagcggt acaccgccgc ttaacacatc
      781 ggccaggatg gtcggcggca gtgccacccg cagcaagcga gccatgctgc acggggtgac
      841 gacactgctt agatagcggt tgttctgggc atccaagagc tgaaagcgct gaccatcggt
      901 cgccagggtc aaaagcggcc ccgtcagtgt gctttccgcc gtcagacgaa gctgggctgg
      961 gcgcttgacc gcgatcgaca catccagctt gaccctgccg ccgccatcgt cgggaacatc
     1021 ggccttggcg tacagcttca tcgactgcac cttcgcctgg ctctttcgca cgtcagacag
     1081 aagctctgcc gcgtttgggg ctggataggg ccgaatgacg accggcgggg gcgggcatcc
     1141 ggtcagcgcc acggtcattc cgaaacagaa ggcaaggtgg agcgctggtc gtcgcagcgg
     1201 ggtttcgttt tttcgcgcac gggccgtcgg gtcaagtgct tggttcaaga tgctcaccac
     1261 cttgcaatcg tcggacgaag gtactggaaa gcggtggctt gcgccagctt ccgtcgaccc
     1321 ccctgtaagc gtactgcggc actgtggggg cgggcttcgg cttgtagtcg tggccatcca
     1381 cccaatgagt gaaccgggtg gggtccggac cccaccaaaa cgactcgatg agtcggcggt
     1441 tttgggtaaa gacccaaggc aaacgactcg acgagttgct ggtcttgggt aaagacccca
     1501 ggcaaacgat tcgatgagtc gccagtttgg ggtccggacc agggtctatc gacagatcgt
     1561 gctttggggc cttggtccgg accccgccca aacgactcga tgggtcgatt gcgccctggg
     1621 atgacccaaa ccaaacgact ttgggcggcc caggcagcgc tggtcgcccc ggcgatgcaa
     1681 catctgcaat gggtttgctt ggagagaacg accggatggg agtaccttgg ccagtcatga
     1741 cgacccatcg catcttcaat ttctctgccg gtccggctgt tctccccgag gaagttctac
     1801 ttgaagcgcg cgacaacctg ctgtcgcttg ggtctagtgg catcggcatc ctcgaatgca
     1861 gtcaccgctc caaggacttt gaggcgatca tcggcgaggc ggaggccaac ctgcgcaagc
     1921 tgctgggcat tccgtccggc taccatgtgc tgtttctgtc cggtggggcc accacgcagt
     1981 tctcgatggt gccgatgaac ctgcttctgc ctgggacagt tggtgactac gtcctgactg
     2041 gtgagtggtc gaagaaggct cttttggaag cgcagcgggt cggggaaacc catgtggcgg
     2101 cgaccaccga agccgaacag ttccgtcgca ttcccaaaaa cgaagagctg cactgctcgg
     2161 atcgttccgc ctacgtccat accaccagca acaacaccat ctgggggacc gagtggcatc
     2221 acgagcctga tgctggcgga cggcccatcg tctgtgacat gtcgagcgac tttttgtcga
     2281 ggccggtcga cgttgcgcgc tacggactga tcttcgctgg ggcccagaag aacgcaggcc
     2341 ctgcgggtgt cacggtggtg attgtccgcg acgacatgct gcggctcgac aaggcggccc
     2401 aggccaagct gcccgtcatg atgaactacc gcacgttcgt cgaccagaag tcgctttaca
     2461 atacgccgcc ggtcttcccc atctacatcg tccacttggt gctgcgctgg ctcgtgcggt
     2521 ttggcggtct ggcgaagatg gccagccaca accaggaaaa agcccagttc atttacgacg
     2581 tcatcgatga tgcgtcgggc ttctaccgcg gccatgcgac ccgtgacagc cgttcgctga
     2641 tgaacatcac cttccgtttg ccaagcgaag ccctggaaga caagttcgcc aaggaagcca
     2701 aagccgctgg catggacggt ctaaagggtc atcgctccgt cggaggtatc cgcgcttcgg
     2761 tgtataacgc tttccccatc gccggtgccc aggcgttggc caacttcatg cgcgactttg
     2821 ctgcgcgcaa cgggtagtcg ggaacagact tgccattccg tctgtttagt gacttgattt
     2881 gccccgcttt caagcaagcg ctgccatgtc tcaagacgtc caaccaacag catctgcctc
     2941 gtcgctgacc cccactgggg gggcagtgta tcagaaccac atccggaact tttccatcat
     3001 tgcccacatc gaccacggca agtcgacgct cgccgaccga ctgctcgaag cgtgtggtgc
     3061 attaagtgac cgagagaagc aggaccagtt tctcgacaaa atggagctgg aacgagagcg
     3121 cggtatcacc atcaaggccc agacggtgcg gttgcgctat cgggccaaag acggcaagga
     3181 gtaccagctc aatctcatcg acacccctgg ccacgttgac tttcactacg aggtcagccg
     3241 cagcttggcc gcgtgtgaag gggctcttct ggtcgtcgac gcctcgcaag gggtgcaggc
     3301 gcagacgctg gccaacgtct acgttgcgct cgacagcaac ctggaaatcg ttccggtcct
     3361 gaacaagatc gatctgccgt ccgcggaacc cgaacgtatc cgcaagcaga tcgaggacgt
     3421 gattggcatc gacgcctcga tggcgattcc ggccagcgcc aaagacgggc gtggtatccc
     3481 cgaaatcctg gaagcggtgg tcgaaatggt gccgccgccc aagggcgatc cgacggcgcc
     3541 gctgcaagca ctgatgtttg attcctggta cgacaactat cgcggcgtcg tcatccaggt
     3601 ccgggtcaaa aacggcagtg tcaaaattgg cgacaagatc cagctcatgg cgacgggtaa
     3661 gccctacgag gtcttgggcc tgggcgtttt caatccgcac ccgatcgaga tcaaagagct
     3721 gcgctgtggc gaggtcggtt tcatcatcgc caacatcaag accatcgccg acacccagat
     3781 tggtgacacc atcacccacg ccgaccgccc atgcagtgag ccgctggcgg gctttaaagt
     3841 ggtcaagccg atggtgttcg cgggtatctt cccgacggat aacgccaaat ataccgatct
     3901 tcgcgacgcg ctggagaagc tgcacataaa cgacgccagc tttacctacg accctgagac
     3961 gtcgctggcg ctgggttttg gcttccgctg cggcttcctg ggtcttttgc acatggaaat
     4021 cgtgcaagag cggctggagc gcgagtacga cctggacatc atcaccacgg cgccgactgt
     4081 cgaataccgg gcgacgacga cctcgggaga gacgatcatc gtcgacaacc cagcggcgct
     4141 gcccgacgaa ggcaagctcg attacatcga ggagccgatc atctcggcca gcatctatac
     4201 tccgtcggag tatgttggcg cgctgatcac gctgtgccag gaacgccgcg gcgtgcaggt
     4261 tggaatgacc tacaacggcg aggaccgcgt ggtgctcaag tacgagctgc cgctggccga
     4321 agtggtgttc gggttctatg accgactcaa gtcggtgtcg cgcggctatg catcactcga
     4381 ctacgagctt ctggagtacc ggcgtgccga ccttatccgg ctggatctgc tggtgaacgg
     4441 cgaccgggtc gacgccctgt ctatcatctg tcaccgcgag cgctcgtact atcg
//
LOCUS       DAORGN010000297         4426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10489_length_4426_cov_6.293754, whole genome shotgun sequence.
ACCESSION   DAORGN010000297 DAORGN010000000
VERSION     DAORGN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10489_length_4426_cov_6.293754"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="PKL17_20605"
     CDS             <1..1707
                     /locus_tag="PKL17_20605"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNK47195.1"
                     /translation="AMAPEKQERIARETMEIYAPLAHRLGIQWIKAELEDLSFKYLYP
                     KEFADLSDKLQTYFANRQQYIDEVAQVLRDELQANEIPAVVTGRPKHLWSIYQKTVKT
                     GRDLDQLFDIIAFRAIVKNVRDCYAALGVVHGKWTPIPGRFKDFIALPKPNMYQSLHT
                     SLIGPKAERIEVQLRTDEMHRVAEEGIAAHWKYKEGKPGGSDPDDKKFAWLKQLMEWQ
                     KTLADPTEFIETVKVDLFADEVFVFTPKGDVKGLPTGATPVDLAYLIHTDVGNHCSGA
                     RVNGQLVPLRYKLRNGDTVEIVTSPNQKPTKDWLKFVKTSSAKAKIRHFMRSEQREKA
                     KSLGRDLLDRELRQHDMTFAKCERSGDLVHAVNELRQQSVDEMLMNIGYGKLLPADVV
                     EQLLPEGQRTVRATTEPPSKTANLAKRTPGLKPPGIRVAGEDDVLVRFGKCCSPVAGD
                     PVVGVITRGRGVTVHAKTCSTAMEQPEERRIEIEWDLDHKAARPATVQVVCADKPGIL
                     AQLSQTFHDQGLNISQANCRTLDDDRAVNTFKVSVVDVDQLQKIIRALQKLQGVFAVQ
                     RM"
     gene            1778..2725
                     /locus_tag="PKL17_20610"
     CDS             1778..2725
                     /locus_tag="PKL17_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNK47196.1"
                     /translation="MPKLTHLGCLGAAALLGATLATAHAETSATVLETTASTSVSASE
                     RAYFDSALTETLRAEQFVVTTASDREVIFTEEKRLQSCQTAVCMERIGRLLGSRFVIR
                     ADVAVHRQETPPDAEARKDRSGRVLAPKTAAADWRLKIELFHVDVGEAGSTVTAECLR
                     CDTSAAGHALSELLARAIIEEASRPRGSLLVESKPPGALVFVDGTELGVTPFKRPSYS
                     GKHRLVLRSAGFLSDQRDIEVPESQRLSLKITLAEGTDPAELPTLSDQKSPIYKKWWF
                     WVAIGGAAAAVAGAATAGTVVTAQGPSQNGTPATNHFVF"
     gene            2737..4281
                     /locus_tag="PKL17_20615"
     CDS             2737..4281
                     /locus_tag="PKL17_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47197.1"
                     /translation="MTTLGPPRHRPTLAKDAAPPRLHRVRLGALVAMLPLASCGPSAG
                     PFVTLLLTNFPPETARVALQVTTDDDSKQQQFSGNDSLNVITVEFPVGTRGPVTFDAE
                     TSLDSGCVLGSGTATLPISDDKAYDLKLPIAVDPKRKCTAAPVKLTVRKMGSAQGVIT
                     SSPVTEPCDETCSQQTIDYRAGDQVKLSVQVSGDDIFAGWSGGCTGSLPDCSFTITAD
                     TTVTATINKCQGFCPMPSTGTSADLFAVWGASPSAVYAVGADGTIVKWNGSAWTAMTS
                     GVKTTLRAVTAPRDNTSLVLAGGDNGLLLVWNGSVWGRFATTLPSFQINAIGANKLAS
                     SLYIAGSGGNYLLWDGSKWNPPSGYSGSKTLTGISFMPGTDEHFLSGSSGLLVRYNPS
                     GVFTKYPDQNTKSSANFSAVWAGAQAIYMVGDGGTIVKRKSGDGQDGVTQATNTAANL
                     RSISGANDTTLFAVGDGGTALFGSGSSWTKIPASTSQTLYGVYAVDSSTIIAVGAAGT
                     ALRYKP"
BASE COUNT          961 a         1414 c         1293 g          758 t
ORIGIN      
        1 gcgatggcgc cggaaaagca ggaacggatc gcgcgtgaga cgatggaaat ctacgcgccg
       61 ctcgcgcacc gtctcggtat ccagtggatc aaagccgagc tggaagacct gtcttttaag
      121 tacctgtatc cgaaggaatt tgccgatctg tcggacaagc tccagaccta ttttgccaac
      181 cgacagcagt acatcgacga agtggcgcaa gtgctgcgcg acgagctgca agcgaacgaa
      241 atcccggcag tcgtcacagg acggcccaag cacctgtggt cgatttatca aaagaccgtc
      301 aagacaggac gagacctcga ccaactgttt gacatcattg cgttccgagc catcgtcaaa
      361 aacgtccgcg actgttatgc ggcgctcggc gtcgtacacg gcaagtggac gccgattccg
      421 ggccggttca aagacttcat cgcgctgccc aagccgaaca tgtaccagtc gctgcacacg
      481 tcgctgattg gacccaaggc cgaacgcatc gaagtccagc tccgcaccga cgaaatgcac
      541 cgcgtcgccg aagagggcat cgccgcgcac tggaagtaca aagaaggcaa gcccggcggc
      601 agcgatcccg acgacaaaaa gttcgcctgg ctcaagcagc tgatggaatg gcaaaagacg
      661 ctggccgatc cgacggagtt tatcgaaacg gtcaaagtcg atctgtttgc cgacgaagtg
      721 tttgtattta ctccaaaagg cgatgtcaaa ggactgccga cgggagccac gccggttgat
      781 ctggcgtatt tgattcacac cgacgttggc aaccactgct cgggggcgcg cgtcaacggt
      841 cagcttgtgc cgttgcgcta caagctacgc aacggcgaca ccgtcgaaat cgtcaccagt
      901 ccgaaccaaa aaccgaccaa agactggctc aagtttgtca aaacgtcgtc ggcgaaagcg
      961 aaaatccgcc acttcatgcg cagcgagcag cgtgaaaaag cgaagtcgct cgggcgggat
     1021 ctgctcgatc gcgagctgcg tcagcacgac atgacgtttg ccaaatgtga acgatccggc
     1081 gatctggttc atgcggtaaa cgaactgcga cagcagagcg tcgacgaaat gctgatgaac
     1141 atcgggtacg gcaagctgct tcccgccgat gtcgtcgaac agcttcttcc cgaaggccag
     1201 cgcacggtgc gcgccaccac agaacccccg tcgaaaaccg cgaatctggc caagcgaacg
     1261 cccggactca agccgccagg gattcgcgtc gccggggaag acgatgttct ggtgcgcttt
     1321 ggcaagtgct gttcccccgt cgctggcgat ccagtcgtcg gcgtgatcac gcgcggtcgc
     1381 ggcgtcaccg tccacgccaa gacctgttcg acggcgatgg aacaacccga agaaagacgg
     1441 atcgaaatcg agtgggatct ggaccacaaa gcagcacggc cagcgacggt gcaggtcgtg
     1501 tgtgccgaca agccgggcat tttggcgcag ctttcccaga cgtttcacga tcaagggctc
     1561 aacatctcgc aggccaactg ccgcacgctc gacgacgacc gcgccgtcaa cacgttcaaa
     1621 gtcagcgtcg tcgacgtcga tcaactgcaa aagatcattc gggcgctaca aaagctgcag
     1681 ggcgtttttg cggttcagcg aatgtaaccg cccaaagcga gccttcgagt cggtgtaaaa
     1741 tgaccccatg tcgcatcgcc cagcacggag agtcgcgatg ccgaagctga cccatctggg
     1801 atgcctcggg gccgcggcgc tgctcggtgc aacgctggcg acggcgcatg ctgaaaccag
     1861 tgcaaccgtg ctcgaaacca cggcttcgac gtcggtcagt gcctccgagc gagcctattt
     1921 cgattcagcc ctgacagaaa cgctgcgtgc cgagcagttt gtcgtcacga ccgcaagcga
     1981 tcgcgaagtc atcttcaccg aggaaaagcg actgcaaagc tgtcaaaccg ccgtctgcat
     2041 ggagcgaatt ggccgtttgc tcggcagccg tttcgtgatt cgtgccgatg tggcggtgca
     2101 caggcaggaa acgccgcccg acgccgaagc ccgcaaagac agaagcggtc gagtgctcgc
     2161 gcccaagacc gccgcggctg actggcggct gaaaatcgag ctgtttcacg tcgacgtggg
     2221 tgaagcggga tcgacggtga ctgccgagtg ccttcgctgc gacaccagcg cggctggaca
     2281 cgcactgtcg gagctgcttg cgcgcgcgat catcgaggaa gcgtcccggc ctcgcggatc
     2341 gcttttggtc gaatcgaaac cgcctggtgc actggtgttt gtcgacggaa ccgagctggg
     2401 cgtcacgccg tttaagcgtc cgtcgtacag cggcaaacat cgcttggtgc tgcgcagcgc
     2461 cggcttcctg tccgatcagc gtgacatcga ggtgcccgag tcgcagcgtc tttcgctcaa
     2521 gatcacgctc gccgaaggga ccgatccggc ggagctaccg acgctgtcgg atcaaaagtc
     2581 gccgatttac aaaaagtggt ggttctgggt tgccataggt ggagccgccg cggcggtggc
     2641 aggagcagcg acggcaggca cagtggtgac ggcccaaggc ccaagccaaa acggcacgcc
     2701 agcgacgaat cactttgttt tttagcgaga tgtgacatga cgacacttgg tccgccaaga
     2761 caccgcccga cgctggcaaa agacgctgcc ccaccgcgcc ttcaccgcgt ccgcctcggt
     2821 gcgctcgtcg ccatgttgcc gcttgcaagc tgtggcccgt ctgcgggtcc atttgtcacg
     2881 ctgctgctca ccaatttccc ccccgaaacg gctcgcgtcg cgctccaagt caccaccgac
     2941 gatgacagca agcagcagca gttttccggc aacgactctc tcaatgtcat caccgtcgaa
     3001 tttcccgtcg gcacgcgcgg cccggtaacg ttcgacgccg aaaccagtct ggattcaggc
     3061 tgcgtgctcg gcagcgggac tgcgacactg ccgatttccg acgacaaagc ctatgatctg
     3121 aagctgccga ttgccgtcga ccccaagcgc aagtgcacag ccgcccccgt caagctgacc
     3181 gtgcgcaaaa tgggcagtgc gcagggcgtc atcacgtcgt cgccagtcac cgagccgtgc
     3241 gacgaaacgt gcagccagca gaccatcgac tatcgcgccg gcgaccaagt caagctgtcc
     3301 gtccaggtca gcggcgacga catcttcgcc ggctggagcg gcggttgcac cggttcactg
     3361 cccgattgca gcttcacgat caccgccgac accaccgtca ctgcgacgat caacaagtgc
     3421 cagggtttct gcccgatgcc cagcaccgga accagcgccg acctcttcgc cgtctggggc
     3481 gccagcccat ccgcggtcta cgccgttggt gcggacggta cgattgtcaa atggaacggc
     3541 agcgcctgga ccgccatgac aagcggggtc aaaacgacgc tccgggccgt cactgcgccc
     3601 cgcgacaaca cctcgctggt cctcgccggc ggcgacaatg gccttctcct cgtgtggaac
     3661 ggctcggtct ggggtcgctt cgccaccact ttaccgagct ttcaaatcaa cgcaatcggc
     3721 gcaaacaagc tcgcgtcgag cctgtacatc gccggcagcg gcggcaacta cctgctctgg
     3781 gacggcagca agtggaaccc cccgtcgggt tacagcggca gcaaaacgct caccgggatt
     3841 tcgtttatgc ccggcaccga cgagcatttc ctgtctggtt caagcggcct gctggttcgc
     3901 tataacccga gcggcgtctt taccaaatat ccagatcaga acaccaaaag cagcgcaaac
     3961 ttcagcgccg tctgggccgg tgcccaagcc atctacatgg tcggcgacgg cggcaccatc
     4021 gtgaaacgca agtccggcga cggtcaagac ggtgtcacgc aagcgaccaa cactgcggcc
     4081 aatctgcgca gcatctcggg cgccaacgac accaccttat tcgccgtcgg agacggtggc
     4141 accgcgcttt ttggaagcgg ctccagctgg accaaaatcc cagcgagcac cagccagacg
     4201 ctctatggcg tctacgccgt cgacagcagt accattatcg ccgtgggagc cgccggcact
     4261 gcgctccgtt acaaacccta gcgtggcccg aggatgagcg aacagtcgat acccagacac
     4321 gaacgactag gcgtcttcat cctcggcgcc atccagttcg tcaacatcct cgatttcatg
     4381 atgatcaagt caccggggca cccctagccc cgtcgctgta ccacgc
//
LOCUS       DAORGN010000298         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10519_length_4419_cov_7.139551, whole genome shotgun sequence.
ACCESSION   DAORGN010000298 DAORGN010000000
VERSION     DAORGN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10519_length_4419_cov_7.139551"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKL17_20620"
     CDS             complement(<1..512)
                     /locus_tag="PKL17_20620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK47198.1"
                     /translation="MDYTTADDWYQTVDAPAQAPASPLWAERPNENASLLAQRYEIKG
                     LLGAGGAGSVFRARDLVLGEDVALKVLKRVHPHPAEAVEDLRNEVRLARRVTHRSIAR
                     VYDLVEHDGVYFLTMEFIDGISLAKRLGRGRSKQTILPLHEVITHASQIAEGLYAAHC
                     ADVVHCDLKP"
     gene            complement(578..1453)
                     /locus_tag="PKL17_20625"
     CDS             complement(578..1453)
                     /locus_tag="PKL17_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47199.1"
                     /translation="MQKGNPCRESARSRLGILGSALLFLLLFQTGCLSLRSVSATGAV
                     GTWIYARVGATKDLLLYCDAENLLAGLTTTCPREQTAAAIADIDRYSQVLSEYATQLK
                     NLADNNDARSADPVRTLVWNIQRVSQQGALSIDPSSVAMQQGAASLLALITEESRRRK
                     LETVIKETHPHVMAVCDGLLARVNLLGEPARQLVQSGIPFRIRALQELDRSVKVSDPA
                     SRQQRQATMLALLHVGHDLSASYDALLSYKKAVLAFSRGHQILFDSVTKNRSLRSHDK
                     EIFENLRKELPELLK"
     gene            1479..2018
                     /gene="moaC"
                     /locus_tag="PKL17_20630"
     CDS             1479..2018
                     /gene="moaC"
                     /locus_tag="PKL17_20630"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNK47200.1"
                     /translation="MSSSPKDTLPVASLSHLDEHGRAHMVAVSEKPETLRSARASGRV
                     YMQKATLDLIMAGALPKGDVIQVARIAGISAVKRTADLIPLCHPLRITSVKVEFSPDP
                     IADSATESALSISATVTAMDRTGVEMEALTAVTVAGLTIYDMCKAVDRAMRISEVQLD
                     EKRGGRSGHFVNPSARSPA"
     gene            2052..3647
                     /locus_tag="PKL17_20635"
     CDS             2052..3647
                     /locus_tag="PKL17_20635"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK47201.1"
                     /translation="MTKHGVSPTSAFPSQPAAARESLFAWQNSKRQNFFDHDHDLQAI
                     LHHHLGQETFSREAPGLSAFGAESATVLDDLIRENNRDENLPVLRRFDGIGRRIEEVS
                     FHPSYLEIGRRAYRTGAIARYARPGEERVQLAYSYLLAHHGEGGHLCPFACTAGLIKI
                     IQARGNEALKKHLLPGLFHLDRIDPAHHHGAQFLTELQGGSDVGQNAVRAQKDGDVYR
                     ISGEKWFCSVIDADLYLLTARIDGAPAGTAGLGAFVVPRLLPDGNPNGVFIRRLKYKL
                     GTRSMASGEADFVSALAWPVGDPIGGFKSVVEIVLNTSRVYNAIAACGMMRRAEIEAT
                     TYAAHREAFGQKIAAFPLVKRTLSRISTERRLAVAGTFALIALSDLIALSQADDTLRA
                     VYRLVVNMNKYWTAIRCTSVIRDAIEILGGNGAIEDFSVLPRLLRDSIVIEAWEGTHN
                     VLCAQVLRDLQKLSLHRAFFAYLRERTSKPELLTEIAEGEQAVTRLLALPADDAALEI
                     RDVVDQLMPLWQKVCVAEVLSVV"
     gene            3692..>4419
                     /locus_tag="PKL17_20640"
     CDS             3692..>4419
                     /locus_tag="PKL17_20640"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNK47202.1"
                     /translation="MALFDFKKLDNIFHPQNPTPHPAETHASQPVPAPKPAVPASASA
                     QATIPKATPVVEKPPVVAPTPAPHPTPAADAQAKTPAQAPTPDPAKEAAKVEDPEHRA
                     AVTHQMEVLKEQKTILKPGDKGPNVLALQAQLKQLGLHVEQSGVMDKSTEGYVMALQT
                     GGGLGADGVVGPKTLDQLLKLDQSPQKPAPGNGQAANPDNVDHMPDDPKLAAVYLYNQ
                     QRLKNAKTELNDEQKRDMDQFIKN"
BASE COUNT          909 a         1278 c         1332 g          900 t
ORIGIN      
        1 tccggcttca aatcgcagtg gacgacgtcg gcgcagtggg cggcgtagag tccttcggcg
       61 atttgggagg cgtgggtgat cacttcatgg agcggcaaaa tggtctgctt gctgcgcccg
      121 cgtccgagcc gcttggccag cgagatgccg tcgataaact ccatggtcag gaagtagacg
      181 ccgtcgtgtt cgaccaaatc gtacaccctg gcaatggatc gatgggtgac gcggcgggcc
      241 aggcggactt cgttgcgcag gtcttccacc gcttcggcgg gatgtggatg cacgcgcttt
      301 agcactttga gcgcaacgtc ttcgcccagc accaagtcgc gggcgcgaaa gacgctgcct
      361 gcaccgcctg cacccaagag ccccttgatt tcgtaacgtt gagcgagcag tgatgcgttt
      421 tcgttcggac gttctgccca cagcggactg gctggcgctt gggccggtgc atcgaccgtc
      481 tgataccaat cgtcagcggt agtgtaatcc atcggcgacg ctcctcccca ggaagcaaac
      541 tgtagtttag caaagctgct tctttacagt gtgcgcatca cttcaggagc tctggcagtt
      601 ctttgcggag gttttcgaaa atctctttgt cgtggctgcg caggctgcgg tttttggtca
      661 cgctgtcgaa caggatctgg tggccacgcg aaaacgccag caccgctttt ttgtacgaca
      721 aaagcgcatc gtagcttgcc gacagatcgt gcccgacgtg caaaagcgcc agcatcgtcg
      781 cttgcctttg ctgacggctg gctgggtccg acactttcac gctgcgatcc agttcttgga
      841 gtgcgcggat gcggaatggg attccggact gcaccagctg tcgcgccggc tcccccagca
      901 ggttcactcg cgcaagcagt ccatcacaga ccgccatcac atgcggatgg gtttctttga
      961 tcacggtttc gagcttgcgc cgccgcgact cttcagtgat gagtgcgagc aggctcgcgg
     1021 ccccttgctg catcgccacc gacgatggat caatcgacag cgcgccttgt tgggacacgc
     1081 gctgaatgtt ccataccaag gttcgtaccg ggtcggcgct gcgggcatcg ttattgtctg
     1141 caaggttttt tagctgggtc gcatattcgc ttagcacttg ggaatagcgg tcgatgtccg
     1201 cgatcgccgc tgctgtttgt tctcgcggac aagtcgtcgt cagccccgcc aaaagatttt
     1261 ccgcgtcgca gtacaagagc aggtctttgg tggcgccgac gcgcgcgtaa atccaggttc
     1321 ccacggcccc ggtcgccgac acgctgcgca gggacagaca gccggtctga aacagcaaaa
     1381 ggaaaagcag cgcactgccg agaattccaa gacgtgaccg ggcactttcg cgacaggggt
     1441 ttcccttctg cataggtcgg atgctagcat gcgcgcccat gtcgtcctcc cccaaagaca
     1501 cactcccggt tgcttcgctc agtcacttgg atgaacacgg acgcgctcac atggttgccg
     1561 tgtcggaaaa accggaaacg ttgcggagcg cgcgggcttc cggtcgggtg tacatgcaaa
     1621 aagcgacgct cgacctgatc atggcgggtg cgctacccaa aggcgatgtc atccaggtgg
     1681 cgcggatcgc gggcatttcg gcggtgaagc gaacggcgga tctgattcct ctttgtcatc
     1741 cgctgcgcat tacatcggtc aaagtcgagt tttcacctga tccaatcgca gattccgcaa
     1801 ccgaatctgc actgtcgatt tcggccacgg tcactgcgat ggatcgaacc ggtgtcgaaa
     1861 tggaagcgct cactgcggtg acggtcgcgg gactcacgat ttatgacatg tgcaaggccg
     1921 tcgatcgagc gatgcggatc agcgaagttc agcttgacga aaaacgcggt ggacgcagtg
     1981 gccattttgt caatcccagc gcccgttcac ctgcgtgacg tactctgttt cggttgtgct
     2041 accgtggcgg catgacgaag cacggtgtct cgcccacgtc cgcgtttcct tcccagcccg
     2101 ctgctgcgcg ggagtcgctt tttgcctggc aaaactccaa gcggcagaac ttttttgacc
     2161 acgatcacga tctgcaagcc atcttgcatc accacttggg gcaagagacc ttttcgcgtg
     2221 aagcgccggg tctgtcggcc tttggtgcgg aatcagcgac ggtgcttgat gatctgattc
     2281 gcgaaaacaa ccgggatgaa aaccttccgg tgctgcgccg ctttgatgga attggtcgcc
     2341 ggatcgaaga agtcagcttt catcccagtt atttggaaat cggacgccgc gcctaccgaa
     2401 ctggcgccat cgcacgctat gcccgtcccg gcgaggagcg ggtgcagctc gcgtattcct
     2461 atttgctcgc gcaccacggt gaaggcggac atctgtgtcc gtttgcttgc accgcaggac
     2521 ttatcaagat catccaagcg cgcggaaatg aagcactgaa aaagcatctt ttgccgggac
     2581 tgtttcacct cgacaggatc gacccggcgc accatcacgg cgcgcagttt ttgaccgaac
     2641 tgcaaggtgg gtcagatgtt ggccaaaatg cggtgcgcgc gcaaaaagac ggggatgtct
     2701 accgcatcag cggagagaag tggttctgtt cggtgatcga tgccgacttg tatctgttga
     2761 cggctcggat cgatggagcg ccggcgggaa cggcggggct tggcgccttt gttgtgccgc
     2821 gacttctgcc ggatgggaat cccaacggag tgttcatccg tcggctcaag tacaagctgg
     2881 gaacgcgcag catggcgtca ggagaagccg actttgtctc tgctttggcg tggccggttg
     2941 gcgatccgat cggcggcttc aagtcggtgg tcgaaatcgt tctgaacacc agccgggtct
     3001 acaacgcgat tgctgcgtgc ggaatgatgc gtcgcgccga gatcgaagcg accacctatg
     3061 cggcgcatcg ggaagcgttc ggacagaaaa ttgccgcgtt tcctttggtg aaacggacgc
     3121 tgtcgcgaat ttcgacggaa cggcggctcg cggtggcggg gacctttgcg ctcatcgcgc
     3181 tgtcggatct gatcgcactt tcacaagccg atgacacgct gcgagcggtg taccgcttgg
     3241 tcgtcaacat gaacaagtac tggacggcga ttcgctgcac gagcgtgatt cgtgatgcga
     3301 tcgaaattct gggcggaaac ggtgcgatcg aagacttttc ggtgctgccg cggcttcttc
     3361 gtgacagcat cgtgattgaa gcgtgggaag gaacccacaa cgtactgtgt gctcaggttt
     3421 tgcgcgactt gcagaagctg tccctacacc gcgcgttctt tgcgtacctg cgggaacgga
     3481 ccagcaagcc ggaacttctg accgaaatcg cggagggaga gcaagcggtg acaagacttt
     3541 tggcactgcc tgccgatgac gctgcgctcg aaattcgcga tgtggtcgat cagctgatgc
     3601 cgctgtggca aaaagtctgt gtcgcggagg tattgtctgt cgtgtaaccc ggcgagacaa
     3661 cttcggcaaa agggccagcg aaaactactg tatggccctt ttcgacttca agaagctcga
     3721 taacattttc catccgcaaa atccgacgcc gcacccagcc gaaacgcacg cgagccagcc
     3781 ggttccggct cccaaaccgg cggttcctgc ttctgcttca gcgcaagcca cgattccgaa
     3841 agcgacccct gtggtcgaaa aaccgccggt ggtcgcaccg acacctgcgc cgcatccgac
     3901 gccggctgcc gacgcacaag ccaagacgcc ggcccaggct cccacgccgg accctgccaa
     3961 ggaagccgcc aaagtcgaag atccggaaca tcgggcagcg gtcacgcacc agatggaagt
     4021 gctaaaagaa cagaagacga tcctaaagcc cggcgacaaa gggcccaatg tgctggcgct
     4081 ccaggcgcag ctaaaacagc ttggtcttca cgtcgaacag agcggcgtga tggacaagtc
     4141 gaccgaaggc tatgtcatgg cgctgcagac cggtgggggt cttggcgctg atggtgtcgt
     4201 cggtccaaag acgctggatc agctgctgaa gctggatcag tcgccgcaga agccggcacc
     4261 aggaaacggc caggccgcga atcccgacaa cgtcgatcac atgcccgacg atcccaagct
     4321 cgcggcagtg tacctataca accagcagcg actcaaaaac gccaagaccg aactaaacga
     4381 cgaacaaaaa cgcgacatgg accagttcat caagaactg
//
LOCUS       DAORGN010000299         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10735_length_4366_cov_4.859197, whole genome shotgun sequence.
ACCESSION   DAORGN010000299 DAORGN010000000
VERSION     DAORGN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10735_length_4366_cov_4.859197"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="PKL17_20645"
     CDS             complement(<1..1864)
                     /locus_tag="PKL17_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNK47203.1"
                     /translation="MNQLFGPNTSLRFKLILPPFIVLLFLLSTLSIVGNNWLKDISQR
                     LSQSHADSRASDLAHSPLLTEALKISPEKNLAPLLNSTVDNDPRLLFAVLKLRDQLFI
                     GTRSGAEGEQLSLLLADQKRYSNLAQNVTQRLASIEFSIARRSVAIPGSAEMAELTLA
                     SSLRDQVADYRTSLWIVLGVLGAALFAYAIAAIYVLSTFGRRMDQLTEATSRIEQGEL
                     SVRIGALVPDDIAMVGRAFDIEAERLTSAVNLLQSAASVVERASTDVSQTRAGLSRTL
                     TAQVRDIEEAGQILREVLAGFAASSSQAEQSRDEARNASANCQRLTSSVQDATATVEE
                     AERVVALTSDSLGQVASATADIAKHADTLSDTTSSTASAMLQMKHSISRVRETAITAA
                     SLAEQAGVDAERGTQVLQESLTGMEHIREASRAIGSVTEELEKRVGEIGDVLHLITEL
                     TQRTNLLALNASIIAAQAGAEGRGFAVVADEIKDLARRTANSAGSIDNLIQAVAEGAH
                     AARRAAVAGADAVEAGTSQASEAAKTMTDILSRLRNSAAMSKSIASATDEQARSSAYV
                     TRSIQDVQNHVTEITGKAVDQTRRTEYLQRQVSRLRELVDRLSKGGREERDAMTA"
     gene            complement(1895..3667)
                     /gene="hutU"
                     /locus_tag="PKL17_20650"
     CDS             complement(1895..3667)
                     /gene="hutU"
                     /locus_tag="PKL17_20650"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNK47204.1"
                     /translation="MSIATPTEGFIKSAVQASRTGTLAAPTGATLHCKGWGQEAALRM
                     LCNNLDPAVAERPQDLVVYGGTGKAARNHQALEVIIRELVSLENDETLLVQSGKAIGV
                     LKTHADAPRVILANSNLVPAWANWQHFWELEALGLMMYGQMTAGSWIYIGTQGILQGT
                     FETFAAAARKHFDGTLAGRLVVSAGLGGMGGAQPLAVTLNDGVFLGIDVDASRIRRRV
                     EQAYLDEEARDLDDALSRVRAAQAAKRPLSVGLCGNIADVLPELVRRGVVPDLLTDQT
                     SAHDPLGGYVPAGLDLAAAEALRRSDPERYIERARLSMVNQLVASLQLRQAGTQLFDY
                     GNNVRGQALLHPQAAAHGLDRSSLFAAMPGFVPAYIRPLFCEGKGPFRWAALSGDPAD
                     IERTDAAVLEVMKGSPSEAHLRRWLSMASRRVKFQGLPARICWLGYGERARVGTYFND
                     LVARGELKAPLVIGRDHLDSGSVASPNRETEAMRDGSDAVSDWVFLNALINCAAGATW
                     VSVHHGGGVGIGYSQHAGMVVVADGTDAARRRLERVLTSDPAMGVLRHADAGYHEAID
                     FAAAHGPRVPHLTQPIEPGTTTGS"
     gene            3930..>4366
                     /locus_tag="PKL17_20655"
     CDS             3930..>4366
                     /locus_tag="PKL17_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNK47205.1"
                     /translation="MPKMSFREALNQAMSEEMERDPRVFLMGEEVGHYNGAYKVSQGM
                     LARFGEKRVIDTPIAECGFVGIGVGAAMVGLRPIVELMTFNFSLVAIDQIVNSAAKIR
                     QMSAGQLSVPMVIRGAGGAAHQLGAQHSNCFEAMYCNVPGLKVV"
BASE COUNT          806 a         1413 c         1325 g          822 t
ORIGIN      
        1 tcgccgtcat cgcatcgcgc tcctcacgcc cgcctttgga cagccggtcg acaagctcac
       61 gcagacgcga cacctgccgc tgaagatact ctgtgcggcg ggtctggtcg acggctttgc
      121 cggtgatttc ggtgacgtgg ttttgcacat cctgaatcga tcgcgtcaca tacgccgagc
      181 tgcgcgcctg ttcgtctgtg gccgaagcaa tcgacttcga catcgccgcc gagttgcgca
      241 gccgcgacaa gatgtcggtc atcgtcttgg cggcttcact cgcttgcgac gttccggctt
      301 ccactgcatc ggcaccagcg acggcagctc gacgggcagc gtgggctcct tcagcgacgg
      361 cttggatcag gttgtcgatg ctgcctgccg agttggcggt gcgacgcgcg agatccttga
      421 tttcatccgc gacgacggcg aagccacgac cttcggcacc ggcctgggcg gcgatgatgc
      481 tggcgttgag cgcgagcagg ttggttcgct gcgttagctc ggtgataagg tgcagcacgt
      541 cgccgatttc accgacgcgc ttttccagtt cctcggtgac ggacccgatg gcgcgcgacg
      601 cttcgcggat gtgctccatg ccggtgaggc tctcttgcag cacttgggtg ccgcgctcgg
      661 cgtcgacacc ggcctgctct gccagactcg cggcggtgat cgcggtttcg cgcacccgcg
      721 aaatcgaatg cttcatttgg agcatcgcgc tcgccgtcga tgatgtggtg tcagacaggg
      781 tatcggcgtg cttggcaatg tccgcggtgg cgctcgcgac ttggccgagg ctgtccgatg
      841 tcagggccac gacacgctcg gcttcttcga cggtggcggt ggcatcttgg acgctcgacg
      901 tgagccgttg acagttggcc gatgcattgc gcgcttcgtc tcggctctgt tcggcttgac
      961 tgctcgatgc ggcaaatccg gccagcactt cgcgcaggat ctgcccggct tcctcgatgt
     1021 cgcgaacctg cgccgtcagc gtgcgcgaaa gcccggcgcg cgtctgtgaa acatccgtcg
     1081 aagcccgctc gaccaccgac gctgcgcttt gcagcaggtt cactgccgat gtcagccgct
     1141 cggcttcgat atcaaacgcg cggcccacca tcgcaatgtc gtcaggaacc agagcgccta
     1201 tccgcacgct cagctcgcct tgttcgatcc ggctggtcgc ttcggtcagc tgatccatcc
     1261 gccgtccgaa cgtcgacagc acataaattg cagcaattgc atatgcaaac agcgccgccc
     1321 ccagcacacc cagcacgatc cacaagctgg ttcggtagtc ggccacttga tcgcgcagcg
     1381 acgacgccag cgtaagttca gccatttcgg cagagccggg aatcgccacc gatcgacgag
     1441 cgatcgaaaa ctcaatgctc gccagacgct gcgtcacatt ctgcgccagg ttgctatagc
     1501 gcttctggtc ggcaagcagc agcgacaact gttcgccttc ggccccactg cgcgtgccaa
     1561 tgaagagctg atcgcgaagc tttagcaccg caaacagaag ccgcgggtca ttgtcgacgg
     1621 tgctgttgag cagcggtgcc aggttttttt ccggcgaaat cttcagcgcc tcagtcaaaa
     1681 gcggcgagtg cgccagatcc gaagcgcggc tgtcggcgtg cgactgcgaa agccgctgcg
     1741 agatgtcttt gagccagttg ttcccgacga tagacagcgt gcttagcaaa aacaaaagca
     1801 cgatgaacgg cggcagaatc agcttgaaac gtaagctcgt gttcggacca aacagctggt
     1861 tcatcttctc tcccgccgct tctggcggtc tcttctaaga gccggttgtc gtccccggct
     1921 cgatgggctg ggttaggtgc ggcacccgcg gaccgtgggc cgccgcaaaa tcaatcgctt
     1981 cgtgataccc agcatcggca tgccgcagca cacccatcgc cggatcactg gttaatacac
     2041 gctcaagccg tcgccgtgcg gcgtccgttc catcggcaac cacgaccatc ccggcatgct
     2101 gggaataccc aatgccaacg ccaccgccgt gatggaccga aacccaggtc gctccggcag
     2161 cgcagttgat gagcgcgttt aggaataccc agtcgctcac cgcgtcgctg ccatcacgca
     2221 tcgcttcggt ttcgcggttg ggggaagcca ccgatccgga atccaagtgg tcgcgtccga
     2281 tgacaagcgg ggccttgagc tcgccgcggg ccaccaggtc gttaaagtag gtgccaaccc
     2341 gcgcacgctc gccgtagccg agccagcaaa tccgtgccgg cagcccctga aatttcaccc
     2401 gtcggctcgc catcgaaagc cagcgacgca ggtgcgcttc gctcgggctg cccttcatca
     2461 cttccaacac ggcggcgtcg gtgcgttcaa tgtcagccgg atctcccgac agcgccgccc
     2521 agcggaatgg gcctttgcct tcacaaaaca gcggccttat gtacgcgggc acaaagcccg
     2581 gcatcgccgc aaacagcgac gagcggtcca gtccgtgcgc agccgcctga ggatgcagca
     2641 gcgcttggcc gcggacgttg ttgccataat cgaaaagctg ggtgccagct tggcgcagct
     2701 gcagcgatgc cacaagctgg ttcaccatcg aaagccgcgc ccgttcaatg tacctttcgg
     2761 ggtcacttcg gcgcagcgct tccgccgccg ccaagtcaag ccccgccggc acataaccac
     2821 ccagcggatc gtgcgccgag gtctgatcgg tcaaaaggtc gggcaccacg ccgcgacgga
     2881 ccagctcggg caatacgtcg gcgatgttgc cgcacagacc gaccgacagc ggacgctttg
     2941 ccgcttgcgc cgccctgacc cgcgaaaggg cgtcgtcgag atcgcgcgct tcttcatcca
     3001 aatacgcttg ctcgacgcga cgccgaatcc gcgacgcatc aacatcaatg cccaaaaaca
     3061 caccgtcgtt gagcgtcacc gccagcggct gcgccccgcc catgccgcca agccccgccg
     3121 acaccaccaa acgaccagcg agcgttccgt cgaagtgctt gcgcgccgcg gcggcgaacg
     3181 tctcgaacgt gccttgcaag atgccttggg tgccgatgta gatccacgac ccggcggtca
     3241 tctgcccgta catcatgagc ccaagcgctt cgagttccca aaagtgctgc cagttcgccc
     3301 acgctggcac caggttcgag ttggccaaaa tcacccgtgg cgcgtcggca tgggtcttga
     3361 gcacgccgat ggctttgccc gactgcacca gcagcgtttc gtcattttcg agcgaaacaa
     3421 gctcgcggat gatgacctcg agcgcttggt ggttgcgcgc cgccttgccc gtgccgccgt
     3481 agaccaccaa atcttgtggc cgttccgcga cggcaggatc gaggttgttg cagagcatcc
     3541 gcagcgctgc ttcttggccc cagcctttgc agtgcaaggt ggcgcccgtg ggggcagcga
     3601 gcgttccggt acgactggcc tgtaccgccg actttatgaa gccttccgtg ggggtagcga
     3661 tagacatggc cagatattac gcgagtctag aatatcatca cttgctagcc tgctcctagc
     3721 ttggcatttg caaggcggcg tcggttttgt gggaaagtgc cccgccaacc accgcgcgtg
     3781 cgggcaagca gcttggcggc ggtagaaatt tgatatgctc gccgaagcca aggtcgcgcc
     3841 ctcgcccgcc agagcatcca aaccatgttg ctggcggtca aaaacaaggg tcggaccacc
     3901 caaaggcaac cgaggagaga acacaggcga tgccaaagat gtcgtttcgc gaagcgctca
     3961 accaagcgat gagcgaggag atggagcgcg acccccgcgt gtttttgatg ggcgaagagg
     4021 tcggtcacta caacggcgcc tacaaggtgt cgcagggcat gctcgcccgt ttcggggaaa
     4081 agcgcgtcat cgacaccccg atcgccgagt gtggctttgt cggaattggc gtcggtgcgg
     4141 cgatggtcgg gctgcgtccc atcgtcgagc tgatgacgtt taacttttca ctggttgcga
     4201 tcgaccagat cgtgaacagc gccgccaaga ttcggcagat gtcggcgggc cagctcagcg
     4261 tcccgatggt cattcgcggg gccggtgggg ccgcccatca gctcggcgcg cagcactcga
     4321 actgtttcga ggcaatgtac tgcaacgtgc cgggactcaa agtcgt
//
LOCUS       DAORGN010000300         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_10880_length_4327_cov_6.287687, whole genome shotgun sequence.
ACCESSION   DAORGN010000300 DAORGN010000000
VERSION     DAORGN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10880_length_4327_cov_6.287687"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKL17_20660"
     CDS             <1..253
                     /locus_tag="PKL17_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47206.1"
                     /translation="AKTHALTGNPDRVVDFSWTQSGFRRAMRQRRAQMRQVSAVVMLR
                     QIRAALDRRVQRLVYGTAWWVWSMLRTCSVQRVPFLPWL"
     gene            complement(416..694)
                     /locus_tag="PKL17_20665"
     CDS             complement(416..694)
                     /locus_tag="PKL17_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNK47207.1"
                     /translation="MTKADIIENVYERVGGFSKKDASQIVEAVFDIIKDTLAKGEKIK
                     LSGFGNLVVRDKNARIGRNPQTGDEITISARRVLTFKPSQVLKNALNS"
     gene            1095..2228
                     /locus_tag="PKL17_20670"
     CDS             1095..2228
                     /locus_tag="PKL17_20670"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNK47208.1"
                     /translation="MAKSRTPVEPPSPPPNGHRQNGSKSQAASGPKKGSGPSKGSVEK
                     FYAELDRCWQVMESGDIEEAKRAALKLLRSDGDSPELYVLLGMISAAECNTDEALSHY
                     QHASDIDPEYVEPLLCAAELYLWDIEDFEKAVELCETALDHAEEEEEFVEALLLKAEG
                     EALLGKESAAYASLSELPDILLGDHSYHVRAGRLLLDLGHPDDAESHFQRALEIQPTD
                     PDSLHGLGLCASERGDRKGMIGYYQKVRAADLKLPTPPWGISHEEFSKLIGETMDKLP
                     EQIRKLIANVPVIASDYPSAEQVADGTDPRILGLFSGVPYGDKPTVGGVPHLDTILLF
                     QRNLERVALSHEELEHEIEITLVHEAGHFFGLSEEQLDALGFA"
     gene            2377..3318
                     /locus_tag="PKL17_20675"
     CDS             2377..3318
                     /locus_tag="PKL17_20675"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNK47209.1"
                     /translation="MKVALFFDGKNFYSGWRERAGRQPLDFTALSRWLVRRVGGSVLW
                     GAYYYTGIETGSPIAGSGQAALTSFLDNLEMLPGYFVQRFSRRTKSYHCRECQAENYY
                     TQENEVDTTMVADMLRLAAVGAFDILVLMSGDADHAPAVEGVRALGRQAYVATWGGIG
                     LSRRIRRAAFDHIDLCDSLGDCGRQWPTGEGEDNGSPIILTGTPQITQGEDTGPPSVP
                     PISDNEEVAATVLLDEVRRAESKFPLGYVGVNFFVTRWQASQLDPDPDVRRRLIDKLV
                     REDRIELYTTEDGTKAMRVRKASSQSPRDQERNGADI"
     gene            3302..>4327
                     /locus_tag="PKL17_20680"
     CDS             3302..>4327
                     /locus_tag="PKL17_20680"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HNK47210.1"
                     /translation="MARTFETPKGTRDFLPEEMRIRNAVFERLRRAFRLFGFDELDTP
                     AFEYLEVLTLKSGPAAEREIYSFTDKGDRRLGLRFDLTSSTGRFAASHPELKRPIYRY
                     QIGKVWRYDRPAAGRYREFYQADADIIGSYSMDCEVDLLLLATTVLKEFGLHDYRFVL
                     NHRKILEAQLRRAGIPVDKKADALRALDKFDKIGREAMITEAKNYGIDESSFVAFIEQ
                     LGISADSTGTSGNLAYLERTEALLADDPDGKKATVELRYIVERATELGFADRLTVDPT
                     LARGLDYYTGPIFEAKAHVEKFGTTLSFAGGGRYDDLIGLYGGQAQGAVRFSFGVERL
                     IDLLKEQV"
BASE COUNT          833 a         1206 c         1371 g          917 t
ORIGIN      
        1 ggcgaaaacg catgctttga cagggaaccc agaccgcgtc gtcgactttt cgtggactca
       61 atcgggcttt cgacgggcaa tgcggcagcg acgggcgcag atgcggcagg tctcggcggt
      121 ggtgatgttg cggcagattc gggcggcttt ggatcggcgg gtgcagcggc tggtttacgg
      181 gactgcgtgg tgggtttggt cgatgctgcg gacttgttcg gtccagcggg tgccgttttt
      241 accttggctt tgaccggctt cgcctttttc gtgggcgcag gggcaggggt cgacggcgca
      301 gtcttggcgc gtcggctcgg ggtcttggct ttggaagcgg acgcagcggc ccgcggcttg
      361 gcagtcatgg cacagaagcg agccgctaca ctgccaggcc cgtccctggt cgcgactagc
      421 tgttgagagc gttttttaac acctgactcg gcttgaaggt caggacgcgc cgcgccgaga
      481 tggtgatctc gtcgccggtc tgcgggttac gaccgatgcg cgcattttta tctcggacta
      541 ccaaatttcc aaagcccgaa agtttgattt tctcgccctt ggcgagggtg tccttgatga
      601 tgtcaaacac ggcctcgaca atctgcgagg catctttctt cgagaagccc ccgacgcgct
      661 catacacatt ctcgatgatg tcagccttgg tcatcgtacg gcgttcttgc atcgctcacc
      721 ccacctgtct gggtagaaag cctgcgttac gtctcgactg ggtagagccc ccctctatcc
      781 agtcgtaatt tgaattacca aaagccccct gacatcccgc acacgaggcc gcttttgacc
      841 tggctgcgcg gggaagcact gctgtggttg gcccatcctg ccggcccccc aacgacgacg
      901 gtgcttcgac tttttagcct accgacggga gagcacgatg tcaaagcaat tgttgaagaa
      961 atatgtatcg gaggatacga aacgcggttc ggcagcgggc gcaaacccgt aggggatcgt
     1021 gagggctgga cgtacaaatg gcttgtattt cgagaccgcc tgcgcatgat gacgcgcgag
     1081 gagtttttga gagcatggcc aagtcacgta ccccggttga gcccccgagc ccaccgccca
     1141 atggacaccg tcagaacggt tcaaaaagcc aggcggcttc gggtcccaaa aaaggctcag
     1201 gtccatcaaa aggcagcgtc gaaaaatttt acgccgagct ggatcgctgc tggcaagtga
     1261 tggaatcggg cgacattgag gaggcaaagc gggcggcgct caagctgctg cgctccgacg
     1321 gagattcccc ggaactgtat gtgcttttgg gcatgatctc agctgcggag tgcaacaccg
     1381 acgaagcctt gtcgcactat cagcacgcca gcgacatcga ccccgaatac gtcgaaccgc
     1441 tgctgtgcgc ggcagagctg tatctgtggg acatcgaaga tttcgaaaaa gccgtcgagc
     1501 tgtgtgagac ggcactcgat cacgccgagg aagaggaaga gtttgtcgaa gcgctactgc
     1561 tcaaggcgga aggcgaggcg ctgctcggca aagagtccgc ggcgtatgca tcgctgtcgg
     1621 agctgcccga cattttgctc ggcgatcaca gctaccatgt gcgggcgggg cggctcttgc
     1681 ttgatttggg tcatcccgac gatgccgaga gtcatttcca gcgtgcgctc gaaatccagc
     1741 cgacagatcc cgattcgctg cacgggctcg gcctgtgtgc gagcgagcgc ggcgatcgaa
     1801 aaggaatgat cggctactac caaaaagtgc gtgccgcaga tctgaagctg ccgacgccac
     1861 cgtggggaat ttcgcacgag gagttttcga agctgatcgg cgaaacgatg gacaagctgc
     1921 cggaacagat ccgcaagttg atcgccaatg tgccggtcat tgcgtcggat tatccgtcgg
     1981 cagagcaggt tgcggacggg accgatccgc gcatcctggg tctgtttagt ggcgttcctt
     2041 acggggacaa accgacggtg ggcggcgtgc ctcacctcga tacgattcta ctttttcagc
     2101 gaaatctcga gcgagtggcc ctgtcccacg aagagctgga gcacgagatc gagatcacgc
     2161 tggttcacga agcgggtcac ttcttcggcc tgtccgaaga gcagcttgac gcgctcggtt
     2221 ttgcataaaa cccgccttgg gccgtgcgcg ccggcgtcgc tgacgtcgtc tgcgctggct
     2281 gtcccgcgcc tcagccgcct tcctcgctgt gcttgacgag aaaacggtat tttttgctgg
     2341 gaatctatct gctctttttt gctaggatgc ccggcgatga aagttgcact gtttttcgac
     2401 ggcaaaaact tctattcagg ttggcgtgag cgggctggac gtcagccgct cgatttcacc
     2461 gcactgtcgc gctggctggt gcgtcgcgtc ggcggtagcg ttctgtgggg cgcttactat
     2521 tacaccggca tcgagacggg cagtcccatc gctggttccg ggcaagcggc actgacgagc
     2581 ttcctcgaca acctcgagat gctgcctggc tactttgtgc agcgctttag ccgccgcacc
     2641 aagagctatc actgtcgcga gtgccaggcc gaaaactact acacccaaga aaacgaagtc
     2701 gacacgacga tggtggccga tatgcttcgg ctggctgccg tcggtgcatt cgatattttg
     2761 gtgctgatgt cgggggatgc cgatcacgct ccagccgtcg agggcgtgcg ggcgttgggt
     2821 cgtcaggcct atgtcgcgac gtggggcgga attggtctgt cgcggcggat tcgtcgggcg
     2881 gcgtttgatc acattgacct gtgcgactcg cttggggact gtggccgaca gtggccgacg
     2941 ggcgagggcg aagacaatgg atcgccgatt atcctgaccg gaaccccgca gattacgcag
     3001 ggcgaagaca ccggcccacc gtcggtgccg ccgatttctg acaacgaaga agttgccgcg
     3061 acggtgctgc tcgacgaggt gcggcgcgcc gaaagcaaat ttccccttgg ctacgtcggc
     3121 gtgaattttt ttgtgacgcg ctggcaggcc agtcagcttg atcccgatcc cgacgttcgt
     3181 cgtcgtctga tcgacaagct ggtgcgcgaa gatcggatcg agctgtacac caccgaagat
     3241 ggcaccaagg cgatgcgggt gcgcaaggct tcgtcgcagt ctcctcgtga tcaggaacgc
     3301 aatggcgcgg acatttgaga cgccgaaagg cacgcgcgac tttctgcccg aggagatgcg
     3361 cattcgcaat gccgtgtttg agcggctgcg acgggcattt cggctgtttg gtttcgacga
     3421 gctggatact ccggcgtttg agtatctgga agtgctcacg ctcaagtcgg gaccggctgc
     3481 cgagcgcgaa atttattcgt ttaccgacaa aggcgatcgg cggctgggtc tgcgcttcga
     3541 cctgaccagc tcgacgggac gttttgcagc gtcgcatccc gagctaaaac gtcccatcta
     3601 ccgctaccag atcggcaaag tctggcgcta cgaccgtccg gcggcggggc gctaccgcga
     3661 gttctatcaa gcggacgctg acatcattgg cagctactcg atggactgcg aggtcgatct
     3721 gctgctgctt gcgacgacgg tgcttaagga gtttgggctc cacgactatc gcttcgttct
     3781 caatcaccgc aagatcctcg aagcgcagct tcgccgggct ggaattcccg tcgacaaaaa
     3841 agccgacgca ctgcgagcgc tcgacaagtt cgacaaaatt gggcgcgaag cgatgatcac
     3901 cgaggccaaa aactacggca tcgacgagtc gtcgtttgtg gcgtttatcg aacagcttgg
     3961 gatttctgcc gattcgacgg gaacgtcggg gaatctggcc tatctggaac gcacagaggc
     4021 gctgcttgcc gatgatcccg atggaaaaaa agcgacggtt gagctgcgct acattgtcga
     4081 gcgtgcgacg gagctcggtt ttgccgatcg gctaacggtc gatccgacgc tggcccgtgg
     4141 acttgactac tacaccgggc cgattttcga ggccaaagcc cacgtcgaaa agtttggcac
     4201 gacgctcagt tttgccggtg gtggtcgcta cgacgatctg attgggcttt atggcgggca
     4261 agcgcaaggc gcggtccgtt tttcgtttgg tgtcgagcgc ctgatcgatc tgctcaaaga
     4321 gcaggtg
//
LOCUS       DAORGN010000301         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_11135_length_4265_cov_5.525653, whole genome shotgun sequence.
ACCESSION   DAORGN010000301 DAORGN010000000
VERSION     DAORGN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11135_length_4265_cov_5.525653"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PKL17_20685"
     CDS             <1..1108
                     /locus_tag="PKL17_20685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNK47211.1"
                     /translation="YSWPWIFYINLPVGALALVMVTRFVHEPEDIRAANQAAAAQQRK
                     NVDWMGIALLSVCLSTMQYVLEEGARNDWFESRLITGCAIVALVTLVGFVLRELSTPS
                     PVVDLSLFKNSVFSSGTLIGSVMFAMLMAMTFLLPLFMQEMLGFTATQSGLALMPRSL
                     VMIVAMPIVGRLYNRVSPRLFIGFGILLFAASAYQMSHYTLDTTARSVVSVLLLQGVA
                     FACLFIPLTTVALSQVPRLKMADAAGLNSLLRQIGGSMGLAIFASLISRFATQAKYAM
                     VANLVPGRPEVLSRLAAMKQFLMSRGADLATAEIGARRMLGGLVMQQAMVLSFEKLFL
                     LAGIAFLCVLPLIFFLRAPQLKTTRVAADVHIEA"
     gene            1112..2341
                     /locus_tag="PKL17_20690"
     CDS             1112..2341
                     /locus_tag="PKL17_20690"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNK47212.1"
                     /translation="MEAHESPSGAAAPVLSATVPPITPKRKPLPIVLALFALIGGGVT
                     AYSYLSAGSESTDAAQVAADMLPVATRVSGQVVRVHIAENQLVKKGDLLAELDSLDYE
                     ARVKQAEAELATAQAQAQAASSQVQVVEASSRGGLLSAKALVSGTAAGVGSAAAQIAS
                     ARATVMRAETEMRKAKLDLERARELRAANAVPQERLDAAQIAFDSSQAAVAQAQAQLA
                     VARESKLSAETRVREAKGRLDQSAPVDAQLAVVKAQADLAKARVRSAEAMLDLARLQL
                     SYTKIVAPSDGIASKLTVHEGQLVGQGQPIIELVPTATYVVANFKETQIGKMKPGQTA
                     EVKIDTYPGRAFEAKIESLAGGTGASFALLPADNASGNFVKVVQRVPVRLVWASPPKD
                     VTLRAGLSAEVTVHVGK"
     gene            2344..3708
                     /locus_tag="PKL17_20695"
     CDS             2344..3708
                     /locus_tag="PKL17_20695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNK47213.1"
                     /translation="MRTMFRHVTRAHRADRFLSLLLGVGLATGTGTTGALATEQASRT
                     DTRLLSVAEAEKRALDSNPSLLSVEAKQRANQALTNAGISRMLPSVFLSDEYQHWNQP
                     FVIAFGPQTLQARDADTNTFSASVSQPLLGLVKLVEEYRAQKLATKATEAQIKVVQAA
                     VRKGVRIAFLRHFEAKAMEEIAQQSEKELSEQVTIAEAKLKAGVLTHADVLRVKVAVA
                     NAQQQEIVAHTQAEVARVSILVAMGGSPDDGVQLQAPTELLLTSRGEVGAYGESLSRA
                     WQARPELQTARLVIDARQHQERSRLWSLLPDINAEGGYVRIDGQIFAPQNSAFVGVKA
                     SWPIWTWGTSWFGYRAAQAETESARQELAAQKRQVANEVASELSQSAAARSAVRVAEQ
                     SITSAQEAYRVTDALVKAGSATTTDLLEAQSALAQARLNLVRAQYQLASAYVSIQHAI
                     GDEK"
     gene            3798..>4265
                     /locus_tag="PKL17_20700"
     CDS             3798..>4265
                     /locus_tag="PKL17_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNK47214.1"
                     /translation="MTTTHTPSGQPITLDKATGKLHVPANPIIPFIEGDGTGADIWRA
                     SVRVMDAAVEICYGGDRKIAWLEVLAGEKAFKQTGNWLPDATVDAFRDYLVGIKGPLT
                     TPVGGGIRSLNVALRQMLDLYVCLRPVRYFRGVPSPVKRPEAVDMVIFRENTED"
BASE COUNT          849 a         1248 c         1360 g          808 t
ORIGIN      
        1 ctacagctgg ccgtggattt tttatatcaa ccttcccgtc ggtgcgctcg cgcttgtgat
       61 ggtgacgcga tttgtccatg aaccggaaga catccgcgcc gcgaaccaag cagcagcggc
      121 acagcagcgc aaaaatgtcg actggatggg aatcgcgctt ctgtcggtct gtctgtcgac
      181 gatgcagtac gtactggaag agggagcgcg caatgactgg ttcgagtcgc ggcttatcac
      241 cggctgcgcg attgtcgcac tggtcacgct ggtcggtttc gtcctgcgcg agctgtccac
      301 gccgtcgccg gttgtcgatc tgtcgctgtt caaaaattcg gtgttttcat cgggaacgct
      361 gattgggtcg gtgatgttcg cgatgctgat ggcgatgacg tttcttctgc ctttgtttat
      421 gcaggaaatg ctcgggttca cagcgacgca gtcgggtctg gcgctgatgc cgcgctcgct
      481 ggtgatgatt gtcgcgatgc ctatcgtcgg acgactttac aaccgggtgt cgccgcgtct
      541 gttcatcggt ttcggaatcc tgctatttgc cgccagcgca tatcaaatga gccactacac
      601 gctcgatacc accgcgcgga gcgtcgtcag tgtgctcttg ctgcaaggcg tcgcatttgc
      661 gtgtctgttc atcccgctga cgacggtggc gctgagccaa gtaccgcggc tcaaaatggc
      721 ggatgcggcg gggctcaact ctctgctgcg gcagattggc ggcagcatgg gacttgcgat
      781 ttttgcgtcg ctgatcagcc gttttgcgac ccaagccaag tacgcgatgg tcgcgaatct
      841 tgtgccaggt cgaccagaag tgctgtctcg actggcggcg atgaagcagt tccttatgtc
      901 ccgcggtgcc gacttggcga cggcggaaat tggggccagg cgaatgctgg gtggccttgt
      961 gatgcagcaa gcgatggtgc tgtcgtttga aaagctgttc cttttggcgg gcatcgcctt
     1021 tttgtgcgtg ctgccgctga ttttctttct gcgcgcgcca cagctcaaga ccacgcgagt
     1081 cgccgcagat gtccacattg aggcgtaaat catggaagct cacgagagtc catctggggc
     1141 cgcggctcct gtcttgtctg cgacggtgcc gccgatcact cccaagcgca aaccactgcc
     1201 gattgtgctc gcgctttttg cactgattgg cggtggtgtc acggcgtatt cctacctgtc
     1261 cgcgggttcg gaatcgaccg acgcggcgca ggtggccgcc gacatgctgc ccgtcgcaac
     1321 ccgcgtttct ggacaggtgg tcagggtcca catcgcggaa aaccaactgg tgaaaaaagg
     1381 ggatctgctg gcagagctag acagcctgga ttacgaagcg cgggtcaagc aagccgaagc
     1441 agagcttgcc accgcgcagg cgcaagcgca ggcagcgtcc tcgcaagttc aggttgtcga
     1501 agccagctca cgcgggggac tgctcagcgc caaagcactg gtatcgggga cggcagcagg
     1561 ggttggcagc gcagcggccc agatcgcttc ggcgcgggcg acggtgatgc gggcggaaac
     1621 ggaaatgcgc aaagccaagc tcgatctgga acgagcgcgt gagctgcggg cggccaatgc
     1681 ggttccgcaa gaacgtctcg atgcagcgca gatcgcattt gattcatcgc aagcggcggt
     1741 ggcgcaagca caggcccagc tcgctgtcgc gcgtgaatcc aagctgtcgg cagagacgcg
     1801 ggtgcgggaa gccaaaggcc gcttggatca gagcgcgccc gtcgatgccc agcttgccgt
     1861 ggtcaaagcg caagccgact tggccaaagc acgggtgcga tccgccgaag cgatgctcga
     1921 tctggcgcga cttcagcttt cgtacaccaa aatcgtcgcg ccaagtgatg gcatcgcatc
     1981 caagctgacg gtgcatgaag gacagctcgt cggacaaggg cagccgatta tcgaactggt
     2041 cccgacggca acttacgtcg tcgcaaactt caaagaaacc cagatcggca agatgaagcc
     2101 gggccagacc gccgaagtga aaatcgacac gtatccgggt cgagcattcg aggccaaaat
     2161 cgaaagtctg gcgggcggca ccggcgcaag ttttgccctg ctgcccgcgg acaatgcgtc
     2221 ggggaacttc gtcaaagtcg tgcagcgtgt gccggtgcgg cttgtttggg caagtccacc
     2281 aaaagatgtg acgctgcgcg ccggcctgtc tgccgaagtg accgttcacg ttgggaagtg
     2341 accatgcgca cgatgtttcg tcatgtgacg cgcgcacatc gcgccgatcg attcttgtcg
     2401 cttctgttgg gcgtcggact ggccactggg acgggaacaa ctggggcatt ggcgacggaa
     2461 caggccagca ggacggacac ccgactgctc agcgttgcgg aagccgaaaa acgcgcgctc
     2521 gattcaaacc cgagtctgtt gtcagtcgag gccaagcagc gtgcaaacca agccctgaca
     2581 aacgctggaa tcagccgaat gctgccgtcg gtgtttttga gcgacgaata ccagcactgg
     2641 aaccagcctt ttgtcatcgc gtttggaccg cagacgctgc aagcgcgcga cgccgacacc
     2701 aacacgtttt cggccagcgt cagtcagccg ctgctgggac tggtcaagct cgtcgaggaa
     2761 tatcgagcgc agaagcttgc gacgaaggcg acggaagcgc agatcaaagt ggtgcaggca
     2821 gcggtgcgca aaggcgtgcg aattgcgttt ttgcgtcact ttgaagccaa agcgatggaa
     2881 gaaatcgcgc agcagtcgga aaaagagcta agcgagcaag tcacgattgc cgaagccaag
     2941 ctcaaggccg gtgtgctgac ccatgcggac gttctgcggg tcaaggtcgc cgtcgccaac
     3001 gcgcagcaac aagagatcgt cgcccacacc caagccgaag tcgccagggt cagcattctg
     3061 gtcgcgatgg gtggttcgcc cgacgatggt gtgcaattgc aagcgccgac ggaacttctt
     3121 ctgacatctc gcggggaagt cggggcgtat ggcgaatcgc tgtcgcgggc gtggcaagcg
     3181 cgtcccgagc ttcagacggc tcggctggtg atcgacgccc ggcaacatca agagcggtcg
     3241 cggctgtggt cgcttctgcc ggacatcaat gccgaaggtg gctacgtccg catcgacgga
     3301 cagattttcg cgccgcagaa ctcggctttc gtcggtgtca aagcaagctg gccgatttgg
     3361 acgtggggta caagctggtt tggctatcgc gccgcgcagg ccgaaacgga gtcagcgcgt
     3421 caggagctcg cggcccaaaa acgccaggtc gcaaacgaag tcgcgtctga gctgtcgcaa
     3481 tccgctgcgg ctcgaagcgc cgtccgcgtc gctgaacagt cgatcacaag cgcgcaagaa
     3541 gcgtatcgag tcaccgacgc actcgtcaaa gcggggagcg cgacgacgac ggatctgctc
     3601 gaagcgcagt cggcgcttgc ccaagcgcgg ctcaatctgg tgcgggcgca gtatcagctt
     3661 gcatcggcct acgtttcgat tcagcacgcc atcggcgacg aaaaatagcc agcacgcacg
     3721 ccgccgcgac caggggttta caagcaagga aactcctgtg atcatcgccg ccatccaaac
     3781 actggagact cacgagcatg acgacaaccc acaccccgtc cggccagccg attaccctcg
     3841 acaaggcgac cggcaagctg cacgtccctg caaaccccat catcccgttt atcgagggtg
     3901 atggcactgg cgccgacatc tggcgcgctt cggtccgcgt catggatgcg gcggtcgaga
     3961 tttgttacgg cggcgatcgc aagatcgcgt ggctggaagt cctggccggt gaaaaagcct
     4021 tcaagcagac cggcaactgg ctgcccgacg cgaccgtcga cgcattccgc gattacctcg
     4081 tcggtatcaa gggtccgttg accacgcccg tcggtggcgg cattcgttcg ctcaacgtcg
     4141 cgctgcggca gatgctggac ctgtacgtct gcctgcgccc ggtgcgctac ttccgcggcg
     4201 tgccttcccc ggtgaagcgt cccgaggccg tcgacatggt gatcttccgc gagaacaccg
     4261 aggac
//
LOCUS       DAORGN010000302         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_11157_length_4261_cov_6.103661, whole genome shotgun sequence.
ACCESSION   DAORGN010000302 DAORGN010000000
VERSION     DAORGN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11157_length_4261_cov_6.103661"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /locus_tag="PKL17_20705"
     CDS             complement(<1..2874)
                     /locus_tag="PKL17_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47215.1"
                     /translation="MTQAEGPAARLKETERSLRWLAAVVIFNVVLSTSFQTAMFLTNH
                     KKPDIPLAMLFGSLLTALTTLGLNYLLRKRPAAGALRIILLTLGGLTAVAATWNLKQT
                     SASTFVLYLLVEIATTVGVAATWTYFQAPLEAAQIRWLLPRLGAWGSIGGLVAGTLIP
                     ITLKFVKLKSHWLIWVSALAWLVASALVRMDYAARAPAKRRSTVNQQSHLRQIFTVPL
                     VRWMILGTAGIIWTGALVQYESNAAMQDAWPQETINSITSVMLAVASIGGFATQTLVT
                     TPVLERWGVGLALAILPATIGACMSVYFYATVGAQWSADLSVWVIVMALFLDKTLRPA
                     LHRPAESCLVAALSPTIRPTLLLVLGGIMSPFIKAVGALLLFIAKSASINPSLLTGIA
                     IILAVLVTMLSTRWGSIYAQTLRETLEEGSVEALGTTEAAENLVPMIDGPRLRVLLEA
                     IDTGSPRSRELALDLLRTQRSNLVAKAMQTRLTNKLEPVRIAALRWLADEPDQTLETH
                     LKQRWILPDLSDSERQAILEAAGPLGAALIGDDTARWLTVPNLPLRTAAMAVLLRSED
                     VRHQKVARRALEAMLNSQSTEEITAALRLVHEHKVGEFLPAVLARLGDDDIAVRREAL
                     SCLPAFEDDRARKPLWAALTEPSMAHVAVRALTSCGESIVPNIIAQLDSPDISTVVRQ
                     HLLRALGLIPSQAGVQCLLDHVRHADRNAQLEALKSLNRLRRVLNLPPLTADVIQSFV
                     LSELRWGLHMQRARELLKDRVRPNGLLYREIDAQIDAAQQRVAQTLALLSPKDTMVHI
                     FKALRSPNSPHRDQARELLRTLFKPGPIVNASLKLLDETQPWQPEFFHPPLSISAGQT
                     AESAVEWLKLTAEPWVIASLKYDPDCPRLTSTFPPIPESIERHLDSVLFLKDVGLFET
                     LTNAQLVDVAALAEKVDLPAGRTLFEQGELPDYLYLIRKGKLRV"
     gene            complement(3195..3488)
                     /locus_tag="PKL17_20710"
     CDS             complement(3195..3488)
                     /locus_tag="PKL17_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47216.1"
                     /translation="MRTTVLALLFAALPALTASAQTTAPTADNSVRSAQGSHTVTYKD
                     RTDYTFGEDEEVVGSLVSPDVEVITSRTKTHHSSLVMPRKNFHPELLQSVDLL"
     gene            complement(3625..>4261)
                     /locus_tag="PKL17_20715"
     CDS             complement(3625..>4261)
                     /locus_tag="PKL17_20715"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNK47217.1"
                     /translation="VTAHETTHAVTSTTSNLTYQNESGALNEGMSDIMGSGVEAWVAS
                     GGTAMGNPAMFKHSADTWLLGKEVAGPKLPGGSLRFMDNPTKDGQSKDWYAERIMPGG
                     TDRGGVHLNSGMPNLVHVLMTQGGTHPRGKSTVQVPAIGIESSLRIFYLTNSKLLTAS
                     STFEQARYAMAQAAQTLYGRCTKEVSAVQKAWDAVGVPGVWQPCVRPPSHF"
BASE COUNT          868 a         1355 c         1261 g          777 t
ORIGIN      
        1 gacccgcagc ttgcctttgc gaatcagata cagatagtcg ggcagctcac cctgttcaaa
       61 cagcgtccgt cccgccggca agtcgacttt ttccgccagc gccgccacgt cgacgagctg
      121 cgcattggtc agcgtttcaa acaaaccaac atcttttaga aacagcaccg aatccagatg
      181 ccgttcgatc gattcgggaa tcggcggaaa tgtcgacgtc agacgaggac aatccggatc
      241 gtacttcagc gaagcaatca cccacggctc ggcggtcagc ttcaaccact cgacggcact
      301 ttccgccgtc tgacctgcgg aaatcgacag cggtggatgg aaaaattccg gctgccacgg
      361 ctgcgtctcg tcgagcagct tgagactggc attgacgatc ggacccggct taaacagcgt
      421 tcgcaaaagc tcgcgcgctt gatcgcggtg cggcgagttg ggcgagcgca gcgccttgaa
      481 gatgtggacc atcgtgtctt tgggcgacaa aagtgccagc gtctgcgcca cacgctgctg
      541 cgccgcatcg atctgtgcgt cgatctctcg gtacaatagc ccgtttggcc gcacccggtc
      601 tttgagcagc tcgcgcgctc tttgcatgtg cagaccccag cgcagctccg acagcacaaa
      661 gctctggatc acgtcggcag tcagcggcgg caggttcagc acgcgacgca gacggttcag
      721 cgatttgagc gcttccagct gcgcgttgcg gtcggcatga cgaacatggt ccagcagaca
      781 ctgcacgccg gcctgcgacg gaatcaggcc caatgcacgc agcaggtgtt gacgaaccac
      841 cgtcgagata tccggcgaat caagctgcgc gatgatgttc ggaacgatgc tttcgccaca
      901 gcttgtcagc gcacgcaccg cgacgtgggc catcgacggt tccgtcagcg ccgcccacag
      961 cggcttgcgc gcccggtcgt cctcgaatgc cggcagacag ctaagcgctt cgcgacggac
     1021 cgcaatgtca tcgtcgccaa gacgcgccag gaccgcgggc aagaactcgc cgactttgtg
     1081 ctcgtggacc agccgcagtg ccgcggtgat ctcctcggtg ctttggctgt ttagcatcgc
     1141 ctcgagcgct cgtcgggcga ctttttggtg acgcacatcc tcggaacgca gcagcaccgc
     1201 catcgccgcg gtacgaagtg gcaagttcgg caccgtcagc cagcgcgccg tatcgtcgcc
     1261 aatgagtgcc gccccaagtg gacccgcagc ctcgaggatc gcctgccgtt cgctgtccga
     1321 cagatcgggc aagatccagc gctgcttcag gtgggtttcg agcgtctggt cgggttcgtc
     1381 ggcaagccag cgcagcgcag caatccgcac cggttccagt ttgttggtaa gccgcgtctg
     1441 catcgccttg gcgacgagat tcgatcgctg ggtccgcaaa agatcgagcg ccagttcccg
     1501 cgagcgcgga ctgccggtgt cgatcgcctc gagcagcacg cgcagacgcg gaccgtcgat
     1561 catcggcacc aggttttccg ccgcttcggt cgtccccagc gcttcgacag agccttcttc
     1621 cagcgtctcg cgcagcgtct gggcgtaaat cgatccccag cgcgtcgaca gcatcgtcac
     1681 caacaccgcc aaaatgatcg cgattccggt gagcagcgac ggatttatcg acgccgactt
     1741 ggcaataaac agaagcagcg caccgacggc tttgataaac ggcgacatga tgccaccgag
     1801 aaccagcaaa agcgtcgggc gaatcgtcgg ggacagcgcc gcaaccagac aggattccgc
     1861 cggcctgtgc agcgccggac gcagcgtttt gtcgaggaac agcgccatca cgatgaccca
     1921 caccgacaga tccgcggacc actgcgcacc gacggtggcg tagaaataaa cactcatgca
     1981 ggcgccgatc gttgccggca ggattgcaag cgccagcccg acgccccacc gttccaaaac
     2041 cggcgtcgtc accagcgtct gcgtcgcaaa accgccaatc gacgccaccg cgagcatcac
     2101 cgacgtaatc gagttgatgg tttcctgcgg ccaagcgtcc tgcatcgccg cattggattc
     2161 gtactggacc agcgcgccgg tccagatgat gcccgccgtc cccaggatca tccagcgcac
     2221 gagcggcacc gtgaaaatct gccgcaggtg gctctgctga ttcaccgtcg agcgtcgctt
     2281 ggccggagcc cgcgccgcat aatccattcg gaccagcgca cttgcgacaa gccaagccag
     2341 cgccgacacc caaatcagcc aatgggactt tagcttcaca aatttgagtg tgatcggaat
     2401 cagcgtgcca gcgacgagac caccaatgct cccccaggcg ccgagtctgg gcagaagcca
     2461 gcgaatctgc gccgcctcaa gtggcgcttg gaaataggtc cacgttgccg caacaccgac
     2521 ggtggtggca atttcgacca gcagatacag cacaaacgtc gacgccgatg tctgcttcag
     2581 gttccacgtc gctgccaccg cagtcagccc gccgagcgtc agcaaaatga ttcgcagcgc
     2641 ccccgccgcc ggtcgcttgc gcaaaaggta gttcagcccc agcgtcgtca gtgccgtgag
     2701 caaactgcca aacagcatgg ccagcggaat gtccggcttt ttgtgattgg tcagaaacat
     2761 cgccgtctga aacgacgtcg acaaaacgac gttgaaaatc acaacggcag ccaaccaacg
     2821 aaggcttcgc tcggtctctt tcagacgggc ggcagggccc tcagcttggg tcatgtccct
     2881 ccctgccaga cctgcccaaa gtggctcgac ggagcaatgc gcagaaatgg cggaagatcc
     2941 accaagccgg atgcgcatgc acaacaagcc ggggcagcag agaggttacg tgcccaatgc
     3001 gagcaagaca ttctttacta aggcaatttt actgttctat ctcggcaatt ggaagcgtcg
     3061 gctctgcgcg gacttgcagt gtttttgcaa agccaaaaat cgcgatgccg atagaacgaa
     3121 cgcgaggaaa gcgaaagtgg cagggtggcg agctgcgtcg tgtggatttg acgcactgcg
     3181 tccgacggaa aagctcagag aagatcgacc gattggagca gctccggatg aaagtttttg
     3241 cgcggcatca ccaggctgct gtggtgggtc tttgtgcgag acgtgatcac ctcgacatct
     3301 gggctgacca gcgaaccgac cacctcttcg tcttcgccaa aggtgtaatc ggtgcggtct
     3361 ttgtaggtga ctgtgtggga accctgcgca cttcgtaccg agttatcagc agtcggtgcg
     3421 gtcgtttgcg cagaagcggt caaggcgggc aaagcagcaa acagcagtgc aaggacagtg
     3481 gttcgcatag aaggtctcct tggcctgggg cgttgcgctt ggtccgctga ctcgcccctc
     3541 cgtcgagtca aacgtcaagc gataccggcc ccaaacgaac aactggggcc aaggatctcc
     3601 gctgcgaaaa actttcccgt ccgactagaa gtgcgacggc ggacggacgc agggctgcca
     3661 cacacccggc acgccgaccg catcccacgc cttctgcacc gcagagactt ccttggtgca
     3721 gcgcccatac agcgtctgcg cagcctgcgc catcgcgtac cgagcctgtt caaacgtcga
     3781 gcttgccgtc agcagcttgc tgttcgtcag gtagaaaatg cgcagcgagc tttcgatgcc
     3841 gatcgccgga acctggacgg tcgacttgcc gcgcgggtgg gtgccgcctt gggtcatcag
     3901 cacatgcacc aagttcggca tgccgctgtt gaggtgaaca ccgccgcggt cggttccgcc
     3961 gggcatgatg cgctcggcgt accagtcttt gctttggccg tctttggtcg gattgtccat
     4021 gaagcgcagc gaaccgccgg gcagcttcgg tcccgcgact tccttgccga gcagccaagt
     4081 gtccgccgag tgcttgaaca tcgccggatt gcccatcgcg gtgccgcccg atgcaaccca
     4141 agcctcgacg cccgatccca tgatgtcgct catgccttcg ttgagcgcgc ccgattcgtt
     4201 ctggtacgtc aggttcgacg tcgtgctcgt caccgcgtgg gtggtttcgt gtgccgtgac
     4261 g
//
LOCUS       DAORGN010000303         4248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_11215_length_4248_cov_6.953255, whole genome shotgun sequence.
ACCESSION   DAORGN010000303 DAORGN010000000
VERSION     DAORGN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11215_length_4248_cov_6.953255"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="metK"
                     /locus_tag="PKL17_20720"
     CDS             complement(<1..796)
                     /gene="metK"
                     /locus_tag="PKL17_20720"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNK47218.1"
                     /translation="MSSFIFTSESVTEGHPDKLCDQVSDAVLDACLTQDRHSRVACET
                     LTKTGFVMVAGEITTKAILDFPTIIRDTVKEIGYTDSDMGFDYKTCGVMVAVEQQSPD
                     IARGVDGQGVFNPEEQGAGDQGMMFGFACDDTEELMPMPISLSHRLSRRLAAVRKNGT
                     LPFLRPDGKSQVSVRYENHRPVAVETVVVSSQHAPNIDHKTLTEAIIEEVVRKEIPTH
                     LLDKNTRFLINPTGRFVVGGPMGDSGLTGRKIIVDTYGGWGRHGGGA"
     gene            795..1040
                     /locus_tag="PKL17_20725"
     CDS             795..1040
                     /locus_tag="PKL17_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47219.1"
                     /translation="MGSPKRISSSNEGRFCAKTCSKVNVNEALILSPCEQQQQQHRTS
                     DASAESYVDEQLSPPQVQKRLGRGLHCQDFGGQAAGG"
     gene            complement(1037..1522)
                     /gene="greA"
                     /locus_tag="PKL17_20730"
     CDS             complement(1037..1522)
                     /gene="greA"
                     /locus_tag="PKL17_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNK47220.1"
                     /translation="MADKYPMTKKGLVALKEELKQRKEIERPRIVLAIEEARAHGDLS
                     ENAEYSAAKEAQSHNEGRLSKLEELVALADVVDPSTLSGNKVVFGATVTIEETDSGEQ
                     QTFAIVGEHEGDIKAGKMSYTSPVARALIGHRVGETVQVKTPRGNREYEIVTVKFLPI
                     D"
     gene            1607..4171
                     /locus_tag="PKL17_20735"
     CDS             1607..4171
                     /locus_tag="PKL17_20735"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNK47221.1"
                     /translation="MLSRTKPSNLTLWLSVLVITIVTGFLHREVLFQRAIYHMDDAAD
                     NYYPARVAFRRALHEGTLPSWEPGAMSGWPLLADPYYGYFYPPNVVFYLGPRAGEPPS
                     GDAGGVPSGLGYSAALHMWIAGLGMLWLLRRRTSWAAALFGALAFALSSFLVVRIRHI
                     IFVQAMAWLPWLLWAVEHHLQTQKKRSLAAAAAFAGLLLLAGAHSLLHFLLMPVVVYV
                     GGRIVSFALARPAGQRLGYAAKTFVWVGLAGLCGCLIGAIALLPTLVQMPLTGRALGT
                     DYYFASTYAWPETKYWQTLLMPDMVGRGEWRGEPWVGKWNHWEIAGYYQGVVSVLLAI
                     PGGIIGFWRRRTLATGDVLAAGDDSGSGLAKEPTFSVELPLLLLLSFAAMSIALGDHG
                     ALHPWLFRYFPLYAALRCPSRALSILVLTMPLLSAIGADVLLGGSRGKKWWLLGLGVA
                     LALAAWFIGIRHQAELFRQAMLAPLSKQLGLLAKAHASLYIACFAGVALLRIIGQAAG
                     PLSLLLLAALTVSDQTRIDRGYLHPQPIDYAYGTERFSAVNWLLQNQPPVASSDPSSP
                     PLYDRFVSDPRGPFRLLALGETVGRPSAAGYGSIQLWRYSHLLYILNHGQPYPHRRLK
                     EDLAAAMLWRLDSPLVDMLNVRYLIGSSPPSPKWVERFRPPLGQPPPARYEPYWDPLL
                     AVFENTQVMPRAFVAYQARLASTQDEEAQLVARPDFDPHREIILGRPSGKDAKTQPVP
                     SIENQNRAHSVAQIVSQKRHQVTIDATVSAPGVLVLADAYHPDWSVTVDGKPDKLWPV
                     NLALRGVLLSPGSHRIEMQYRDRGLRLGMLLSLIGLLAALWLYRYGARKDREKVHV"
BASE COUNT          699 a         1237 c         1367 g          945 t
ORIGIN      
        1 acgcgccgcc accgtgacgg ccccagccgc cgtaggtatc gacgatgatc ttgcgaccgg
       61 tcagacccga atcacccatc ggtccaccga cgacgaaacg acccgtcgga ttgatcagga
      121 agcgggtgtt tttgtcgagc aggtgcgtcg ggatttcttt gcggacgact tcttcgatga
      181 tcgcttcggt cagcgtcttg tggtcgatgt tcggggcgtg ctggctcgaa acgaccacgg
      241 tttccaccgc gacggggcgg tggttttcat aacggaccga cacttgcgat tttccgtcgg
      301 gacgcagaaa cgggagtgtg ccgtttttgc gcactgccgc cagccgccgt gacagccggt
      361 gcgacagcga gatcggcatc ggcatcagct cctcggtgtc gtcgcaagcg aagccaaaca
      421 tcatgccttg gtcgccggcg ccctgttctt caggattgaa gacgccctga ccgtcgacgc
      481 cgcgggcaat gtctggcgac tgctgttcga cggcgaccat aacgccgcac gtcttgtagt
      541 cgaagcccat gtccgaatcg gtgtagccga tctctttgac ggtatcgcgg atgatcgtcg
      601 gaaaatcgag gatcgccttg gtggtgatct cgccggctac catcacgaag ccagtcttgg
      661 tcagcgtctc gcaggcgacg cgggaatggc gatcctgggt caagcaggca tcgagcactg
      721 catcggagac ctgatcgcac agcttgtcgg gatgtccctc agtgacggac tccgaggtga
      781 agatgaagga agacatgggc tctcctaaac gaatctcgtc gagtaatgaa ggccgttttt
      841 gtgccaaaac ctgctcaaaa gtcaacgtaa atgaagcctt gatcctcagt ccctgcgaac
      901 agcagcagca gcagcaccgc acaagcgatg caagcgccga gagctatgtc gacgagcagt
      961 tgtcgccgcc gcaggttcaa aagcggttgg gtcgtgggct tcattgccaa gactttgggg
     1021 gtcaggcagc gggcggctag tcgatgggca gaaacttgac ggtgacgatc tcgtattcgc
     1081 ggttgccacg aggcgtcttg acttgcaccg tctcaccgac gcgatgaccg atgagagcgc
     1141 gtgcgaccgg cgaggtgtag ctcatcttgc cggctttgat gtcgccttcg tgttcaccga
     1201 cgatggcgaa cgtctgctgt tccccggaat cggtctcttc gatggtgacg gtggcgccaa
     1261 agacgacctt gttgcccgat agtgtcgatg gatcgacgac atcggccaac gcgaccagct
     1321 cctcgagctt gctcagtcga ccttcgttgt gcgattgggc ttctttggca gcgctgtact
     1381 cggcgttttc cgaaagatcg ccgtgcgctc gcgcttcttc gattgccaaa acgatgcggg
     1441 gccgctcgat ttctttgcgc tgtttgagtt cttccttcag cgccacgagc cccttcttgg
     1501 tcattgggta cttgtcggcc atgcgggaca gagcatgcct gggaccgcgc aaacgatgca
     1561 agcagcaaaa accgtccgaa aatcccgcta agatgccggc gtcgacatgc tctctcggac
     1621 aaaacccagc aatctgacgc tctggctttc ggtgctggtc atcacgatcg tcaccggctt
     1681 ccttcaccgc gaggtgctgt ttcagcgcgc catctatcac atggacgacg cagcggataa
     1741 ctactatccg gcccgcgtcg cgtttcgtcg cgccctgcac gaagggacgc tgccgtcgtg
     1801 ggaaccgggt gccatgtcag gctggcccct gctcgctgac ccgtattacg gctattttta
     1861 tccgcccaac gtggtgtttt atctggggcc gcgcgcaggt gaacccccgt cgggggatgc
     1921 gggtggagtg ccgtcaggtc tcggttattc ggcggcgctg cacatgtgga ttgccgggct
     1981 tgggatgctg tggcttttgc gacggcgcac gtcgtgggcg gcggcactgt ttggtgcgct
     2041 ggcgtttgcg ctgtcgtcgt ttctcgtcgt gcggattcgc cacatcattt ttgtgcaagc
     2101 gatggcgtgg ctgccgtggc tgctgtgggc cgtcgaacat cacctgcaaa cccagaaaaa
     2161 gcgatcgctc gcggcagcgg cggcgtttgc tggactgctg ctgctcgctg gggcgcactc
     2221 gctgctgcac tttttgctga tgcccgtcgt ggtgtacgtc ggcggacgga ttgtttcgtt
     2281 tgcgcttgcc cgacctgcgg gtcagcgact gggctatgcg gcaaagacgt ttgtgtgggt
     2341 cggtttggct gggctgtgcg gctgcctgat cggcgccatt gcgcttttgc cgacgctcgt
     2401 acagatgccg ctgacggggc gggcgctcgg gaccgattac tattttgcgt cgacgtatgc
     2461 gtggccagaa acgaagtact ggcagacgct gctgatgccg gacatggtcg ggcgcggcga
     2521 gtggcgaggc gagccgtggg tgggcaagtg gaaccactgg gaaatcgccg gttattacca
     2581 gggcgtcgtg tcggtgcttc tggcgattcc gggcgggatt atcgggtttt ggcgacggcg
     2641 aacgctggcg acaggcgatg ttttggcggc gggcgatgac agcggatccg gtctggcaaa
     2701 agaacccacg ttttccgtcg agctgcccct tttgctgctg ctgtcctttg cggcgatgtc
     2761 gatcgcgctt ggtgatcatg gcgcgctgca tccatggctg tttcggtact ttccgctgta
     2821 tgcggcgctg cgctgtccgt cgcgggcgct ttcgatcctg gtcttgacga tgccgctatt
     2881 gtcggcgatt ggtgccgacg ttctgctcgg cggatcgcgc ggcaaaaagt ggtggctttt
     2941 ggggctgggc gtggcgcttg cgcttgcggc ctggttcatc gggattcgcc atcaagccga
     3001 gctgttccgt caggccatgc tcgcgccgct tagcaaacag ctaggtcttt tggccaaagc
     3061 gcacgcctcg ctgtacatcg cgtgttttgc cggcgtggcg ctgctgcgaa tcatcggtca
     3121 ggcagccggc ccgctttcgc ttctgctgtt ggcggcgctg acggtgtcgg atcaaacgcg
     3181 catcgatcgc ggctacctgc acccgcagcc gatcgactat gcctacggga cggaacgttt
     3241 ttcggcggtg aactggctgc tacaaaatca gccacccgtc gcgtcatccg atccatcgtc
     3301 gccgccgctc tacgatcgct ttgtttccga tccgcgcgga ccgtttcgtc tgctggcgct
     3361 gggcgaaacc gtcggtcgac cgagcgcggc tggctatggg tccattcagc tttggcgcta
     3421 ttcccacctg ctctacatcc tgaaccacgg gcagccgtat ccgcaccgac ggctgaaaga
     3481 ggatttggcg gcggcgatgt tgtggcggct cgattcgccg ctcgtcgaca tgctcaatgt
     3541 gcgctacctg attggtagct ctcccccgag ccccaagtgg gtcgagcgat tccgtccgcc
     3601 gctcggtcag ccgccgcccg ctcgttacga accgtattgg gacccgctgc tcgcggtgtt
     3661 tgaaaatacg caggtgatgc cgcgcgcgtt cgtcgcttac caggcgcggc tggccagcac
     3721 gcaggacgaa gaagcgcagc ttgtcgcgcg gcccgatttt gatccccatc gcgagatcat
     3781 cctcgggcgt ccgtcgggaa aagatgccaa gacgcagccg gtgcccagca tcgaaaacca
     3841 aaaccgagcc cacagcgtcg cgcagattgt ttcgcaaaag cgtcaccaag tgacgatcga
     3901 tgccacggtg agcgcgcccg gcgtgctggt tctcgccgat gcgtatcatc ccgactggtc
     3961 ggtgacggtc gatgggaagc ctgacaagct gtggccggtc aatttggcgc tgcgcggggt
     4021 gctgctgtcg cctggttccc accggatcga aatgcaatat cgcgatcggg ggctgcggct
     4081 cgggatgctg ctctcgctga ttgggctttt ggcggcgttg tggctttacc gctacggcgc
     4141 acgcaaagat cgcgagaagg ttcatgtata atcgcgtcta ctgctaggag tcgacgtgcc
     4201 tcggatcttt gtttctcaga agcgtgtgca gcagtggact gaagaagg
//
LOCUS       DAORGN010000304         4222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_11343_length_4222_cov_5.243101, whole genome shotgun sequence.
ACCESSION   DAORGN010000304 DAORGN010000000
VERSION     DAORGN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11343_length_4222_cov_5.243101"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKL17_20740"
     CDS             complement(<1..306)
                     /locus_tag="PKL17_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNK47222.1"
                     /translation="MHSHSVHSQDVSIAYRVIGAGPRDVMLVHGWMVSGAVYDDLVGA
                     LDTEGLRLILVDLRGSGKSGRPQSGYTIEQYAKDVLAVADAVGSRKFMLVGHSMGGQI
                     "
     gene            471..1355
                     /locus_tag="PKL17_20745"
     CDS             471..1355
                     /locus_tag="PKL17_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK47223.1"
                     /translation="MAKSSRRPTGTEGHAETTQEIPAPYYIIRGGSAGRERLRILSRV
                     MRDATCGLFDRVGARTGWSYLDVGCGGGDVTRELARRAGPLGRVVGEDIDEVKLAMAR
                     REASEQGFPHIDFRYRDVYAPLAPSEQATQGGFDFVYVRFLLTHLPDPRTALARLVER
                     LRPGGVLVVEDIDFSGHICHPPHPSFDRYHLLHIESQRRRGGDAMIGPKLPAMLTDSG
                     LADVQVRIEQPVGKDGEVKLIPAITMENIGEAVLRDGQCSPSELATIIEQLYAIATDR
                     QTVTGMPRIFQTWGYAAP"
     gene            complement(1378..1584)
                     /locus_tag="PKL17_20750"
     CDS             complement(1378..1584)
                     /locus_tag="PKL17_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47224.1"
                     /translation="MPTAAHIIYIPAMLCLGFVLGYIFGGRATQAALADEQARAEAKA
                     RRRAARAAEQAAAESSTQSDSSGS"
     gene            complement(1588..1998)
                     /locus_tag="PKL17_20755"
     CDS             complement(1588..1998)
                     /locus_tag="PKL17_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47225.1"
                     /translation="MRSGSWGLSAVLFALALWGPIPSYADEVPSRPSVASKPAEANPA
                     LAEPRTGAPAGQPAVQPAAPQPDKDGFVPESRPMNGPTVDESIPAGPLVAAAYGFLWL
                     AVLGYVLMTGRGLRKVDADLAELDAKISRLSQKS"
     gene            complement(2033..2734)
                     /gene="ccsA"
                     /locus_tag="PKL17_20760"
     CDS             complement(2033..2734)
                     /gene="ccsA"
                     /locus_tag="PKL17_20760"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNK47226.1"
                     /translation="MDETKPPQSFSSTQLGLIALVMLGLLIAPQLVVSAPNEPTMGLV
                     QRIFYYHVPCAWQTMIGAFIAGGASAMYLFRGSQKARQVAIAAAELAVLFGLCVMITG
                     PLWARKAWGVWWQWDVRLTTTALLWIIFVAALFAERYGGPGGPRLSAGLMVFGAADVP
                     MIYISVSLWRTIHPKTSVVPTLDSSMRVALWTSVLTFTLLFAVLMSLRLRLQRQQDRL
                     AELRMKAEDAGLLDE"
     gene            complement(2747..3988)
                     /gene="lepB"
                     /locus_tag="PKL17_20765"
     CDS             complement(2747..3988)
                     /gene="lepB"
                     /locus_tag="PKL17_20765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNK47227.1"
                     /translation="MSEEEKAKGAEVALPSGQRPPAWLRVGLDHLWVLALPLLLGLFL
                     LVVHFTNHIDLTEVPPHPRIVQLAIALAILSVLFGIPYLMFRQIIDQTRDDLLLHRDA
                     KLSVRDAERRFARYGKLLKPEARDAVSAAIAAVDKALGGEDNDELIKTLHGLDQVLNK
                     HLSFARKSPLQEYGESIGGAVLIALLLRAFTVEAFKIPSGSMIPTLQVGDHIFVNKLL
                     FGLRIPFTNIKFGHHLREPRRGEVIVFVFPHDQQKDFIKRIAAIPGDTVEVCDERVSI
                     NGKPLFREPASGICEYDDFDEERPGARWTHQICRGFREKNGNETYRVVQDVQPSLHPC
                     QSWVVPPEHVFVMGDNRDHSHDSRFWCEQRGLDGSQLCPPADKVSFVPYEMIKGKAWF
                     IWWSAGKASSVRLNRLFTSIH"
     gene            complement(3985..>4222)
                     /locus_tag="PKL17_20770"
     CDS             complement(3985..>4222)
                     /locus_tag="PKL17_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNK47228.1"
                     /translation="PPQQFEVAIQGAIGNRVIARTTVKALRKNVTAKCYGGDISRKRK
                     LLERQKEGKKRMKQIGSVEIPQEAFLAVLKVDEK"
BASE COUNT          862 a         1383 c         1216 g          761 t
ORIGIN      
        1 aatctgtccg cccatgctgt gaccgaccag catgaacttg cggctcccga cagcatcggc
       61 gaccgccaag acatccttgg catactgctc aatggtgtag ccgctctgcg gacgacccga
      121 cttcccactg ccgcgcaagt cgaccaggat caggcgcaat ccctcggtat ccagcgcccc
      181 cacgagatcg tcgtaaacgg cgccagacac catccagccg tggaccaaca tcacatcccg
      241 tggtccggct ccaatcacgc gataggcgat actgacatct tgactgtgta cagagtgact
      301 atgcatggga gtctcatcat accgcgcatt gccgatgctt gggtagtttc gagtgaactg
      361 cggtatgatc agacaggtac tcgacgccaa gctgtgcccg gtggtgcaat cgcagcgcaa
      421 gactgccgga gatgagcaga ggcggaacgg ggaaaagcaa ggatgcggtc atggcaaaaa
      481 gctcgcgccg cccgaccgga acagaaggtc acgccgaaac gacgcaggaa attcctgccc
      541 cgtactacat cattcgcggt ggcagcgccg gacgggaacg gctgcgcatc ctgtcgcggg
      601 tgatgcgcga cgcaacgtgc ggcttgttcg atcgggtggg cgcccgtact ggttggtctt
      661 atctcgatgt cggctgcggc ggcggtgatg tcacccgtga actggcccgc cgcgctggcc
      721 ccctaggccg ggtggtcggc gaggacatcg acgaagtaaa gcttgccatg gccaggcggg
      781 aggcgagcga acaagggttc ccccacatcg atttccgcta ccgcgacgtc tacgccccgc
      841 tcgcaccgtc tgagcaggcg acccaaggcg gcttcgactt tgtctacgtc cgcttccttt
      901 tgacccacct gccagatccc cgcactgcgc tcgcccggct cgtcgagcgg ctgcgaccgg
      961 gcggcgtcct tgtggtcgaa gacatcgact tctctggcca catctgtcat cccccccacc
     1021 cctcgtttga ccgctatcac ctgctgcaca tcgaatcgca gcgacgacgc ggtggtgatg
     1081 cgatgatcgg ccccaagctg cccgcgatgc tcaccgattc cggcttggct gatgtccagg
     1141 tccgcatcga gcagccggtg ggcaaagatg gcgaggtgaa gctgattccg gccatcacca
     1201 tggaaaacat cggtgaagcg gtgctgcgcg atggccaatg cagcccctcc gagctagcga
     1261 ccatcatcga acagctttac gccatcgcca ccgatcgaca aacagtgacc ggcatgcccc
     1321 gcatcttcca gacctgggga tacgccgcgc cataaggcgt gtcccaaacc gcccgcgcta
     1381 actgcccgaa gaatccgact gggtggatga ctcggctgca gcttgctccg cggcacgggc
     1441 ggcacgccga cgggctttgg cttcggcgcg ggcctgctca tcggcaagcg cggcctgggt
     1501 ggcgcgtccg ccaaagatgt agcccaggac aaagcccaga cacagcatcg ccgggatgta
     1561 gatgatatgt gccgccgtgg gcatcgctta gctcttctgc gaaagccggc tgatcttggc
     1621 gtccagctct gccaaatcgg catccacctt gcgcagaccg cggcccgtca taagcacgta
     1681 gccaagcacc gcaagccaaa ggaacccata ggctgccgcc accagcggac ccgcgggaat
     1741 cgattcatcc acagtcggcc cgttcatcgg tcggctttcc ggtacaaagc catctttgtc
     1801 gggttgtggg gctgccggct ggaccgcagg ctgtccggca ggtgcccctg tgcgcggttc
     1861 cgccaaagcc gggttcgctt cggctggctt cgatgcaact gacggacggc tcggcacttc
     1921 atcggcatag cttgggatcg ggccccaaag cgccagtgca aacaagaccg ccgacaaacc
     1981 ccagcttccg ctccgcatcc tttttacgat tccttgtcgc aaaagtggtc gtttactcat
     2041 cgagcagccc cgcatcttcc gccttcatcc gtagctctgc cagccgatcc tgctgacgct
     2101 gaagccttag tcgcagggac ataagcaccg caaacagcag cgtaaaggtc agcaccgatg
     2161 tccacagcgc cacccgcatc gacgaatcga gcgtcggcac cactgaagtc ttgggatgga
     2221 tcgtccgcca cagcgacacc gagatgtaaa tcatcggaac atccgccgca ccaaacacca
     2281 tcagacccgc cgacagccgg ggtccgcccg gaccgccgta ccgctctgca aaaagtgccg
     2341 ccacaaagat gatccaaagc agtgctgtcg tcgtcagccg cacgtcccac tgccaccaca
     2401 cgccccaggc tttgcgcgcc cacagaggcc ccgtgatcat gacgcacagt ccgaacagca
     2461 ccgccagctc cgccgcggca atcgccacct ggcgggcctt ttgcgagccg cggaacagat
     2521 acatcgcact cgcaccgccc gcgatgaacg ccccgatcat cgtctgccag gcgcagggta
     2581 cgtgatagta aaagatccgc tgcaccaggc ccatcgtcgg ttcgtttggg gccgacacca
     2641 caagctgcgg agcaatcaaa agaccaagca tcaccagcgc gataagccca agctgggtgc
     2701 tggaaaacga ctgcgggggt tttgtctcgt ccatcaagcc caccctctag tgaatcgagg
     2761 taaacagccg attgagtcgc acggaagacg cttttcctgc cgaccaccag atgaaccagg
     2821 ccttgccctt gatcatttcg tagggaacaa acgacacctt gtcggcagga gggcaaagct
     2881 ggctgccatc gagcccgcgc tgctcacacc agaagcggct gtcgtggctg tgatcgcggt
     2941 tgtcacccat cacaaagacg tgctcggggg gcaccaccca gctctggcag ggatgaaggc
     3001 tcggctgcac atcctgcacg acccggtagg tctcgttgcc gttcttttcg cggaagccgc
     3061 ggcatatctg gtgggtccag cgtgcgccgg gacgctcctc gtcgaagtcg tcgtattcac
     3121 agatgccgct ggctggttcg cggaagagcg gcttgccgtt gatgctcacc cgctcatcac
     3181 agacttccac ggtgtcacca gggatggcgg caatccgctt gatgaagtct ttctgctgat
     3241 cgtgcgggaa cacgaacaca atcacttcgc cccgacgcgg ctcgcgcagg tgatgaccaa
     3301 acttgatgtt ggtaaacggg atgcgcagac cgaacagcag cttgttgacg aagatgtgat
     3361 ccccaacctg aagcgtcggg atcatcgatc cggacggaat cttgaacgct tcgaccgtaa
     3421 aggcccgcag aagcagcgcg ataagcaccg caccgccaat cgattcgccg tattcctgaa
     3481 gcggactttt gcgcgcaaac gaaaggtgct tgtttagcac ttggtccaga ccgtgcagcg
     3541 tcttgatcag ctcgtcgttg tcttcgccgc cgagcgcctt gtcaaccgcg gcaatcgctg
     3601 ccgacaccgc gtcgcgagcc tcgggcttta gcagcttgcc gtagcgagca aaccgccgct
     3661 cggcatcgcg cacagacagc ttggcatcgc ggtgcagcaa aagatcatca cgggtctggt
     3721 cgatgatctg ccggaacatc agatagggaa tgccgaacag caccgacagg atggccagcg
     3781 caatcgcaag ctggacgatc cgcggatgcg gcggcacttc cgtcaggtcg atgtggtttg
     3841 taaagtgaac gacaagcaga aacagaccga gcaaaagcgg cagggccaaa acccacaggt
     3901 gatcgagacc cacccgcagc cacgctggcg gacgctgccc cgatggcaaa gccacctctg
     3961 ccccctttgc cttttcctcc tcgctcattt ttcgtccacc ttgaggaccg ccaagaaggc
     4021 ttcctgcgga atttcgaccg agccgatctg tttcatccgc tttttgccct ctttctgacg
     4081 ctcgagcagc ttgcgcttgc ggctgatgtc accaccataa cacttggccg tcacgttctt
     4141 gcgcagggcc ttgaccgtgg tacgggcgat gacgcggttc ccgatggcac cttggatggc
     4201 cacttcaaac tgctgcggcg gg
//
LOCUS       DAORGN010000305         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_11547_length_4180_cov_5.543030, whole genome shotgun sequence.
ACCESSION   DAORGN010000305 DAORGN010000000
VERSION     DAORGN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11547_length_4180_cov_5.543030"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKL17_20775"
     CDS             complement(<1..724)
                     /locus_tag="PKL17_20775"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNK47229.1"
                     /translation="MRAASCPSQAELFRAFLESAEPGLLRHIEQCPSCSSEWSALVEL
                     RALGKQLPSYVPDAAELDSQKAQLLAKLESPPTSTTKMVPLRYLALSAAVSAAACLIL
                     LFRLWGGERHPSSDAAGFRATVVTQGGDVHFVHERKQGDEWVRLSNGKLLIEVAPLRK
                     GERFRVIAPDGEVEVRGTAFETEVVEDHLVSVRVVHGRVEVRPHSNRSLILGDGERWQ
                     ARLPTVTSSVPPQPPAEKSVATA"
     gene            complement(663..1313)
                     /locus_tag="PKL17_20780"
     CDS             complement(663..1313)
                     /locus_tag="PKL17_20780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNK47230.1"
                     /translation="MPGKLLHFAAGGGTISERTNEDLVAACGRGDRGALGLLFERLSP
                     GVYRFLSRLCGADTPELDDLVQMTFLEVWRSAAAFRGASSAQTWVYAVAANVGRHFRR
                     GEGRRRQMAVRFSQKPVAEPNSPERNTAQREQIARLDAAVATLPAELRIPFLMVYVEE
                     LPGAEVAKALGIPDGTLWRRLYEARQRLWQKLQEEPHESRELSVASRAISRLFGKR"
     gene            complement(1314..2369)
                     /locus_tag="PKL17_20785"
     CDS             complement(1314..2369)
                     /locus_tag="PKL17_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47231.1"
                     /translation="MKHSPAGQRAFRPCRRRTRLCLAGLWLTLRLSVVTASEPALQSD
                     PVPSLPTAANCPPQVQIVGEPGLHRSLADALSRRAIATTVVPGCPTLRAEVSQKGGLF
                     KLVLQQDVPREERLVADVATAVALIETWLRSDISAPLLSAMLAPPMPEPEQPLSPVPE
                     PLPPTLPRATLELAPEAGIDLKSASWFGGRFHGCLYVGRLCLGTTLRMAGDLPRFVSI
                     ETLLSRLSADLLVDAELPVTVGRFTFAPGIGVGAGMVRSEFRLNPGAPHSEEEDEGLL
                     SGPLSITGGGLRAEARFSTAVALYRNLLLSVMLAADGAFLDSGNLLVRLGTKRTLAVP
                     TTAWGLLRGGLGLRWSL"
     gene            complement(2403..2924)
                     /locus_tag="PKL17_20790"
     CDS             complement(2403..2924)
                     /locus_tag="PKL17_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47232.1"
                     /translation="MSLFRSSLVLNKARWGLLLPLLLWFSGCSEVNVDAVPGAAAGCE
                     APSEDNLVPQAPMLPGRDCLACHVAGSQAGDLPWTAGGTVYASGSSTCNTGGLEGVKV
                     ELADETRKVLITLTTNRAGNFFTAEKLLYQTMIARVSKDGKVKEMTQPVTTGNCASCH
                     RPGGAAGGRIYLN"
     gene            complement(2943..3599)
                     /locus_tag="PKL17_20795"
     CDS             complement(2943..3599)
                     /locus_tag="PKL17_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47233.1"
                     /translation="MAWIASYSTANAESPPAPATTAPATTAPATTAPATTAPATGSDD
                     LSFNLLDDGPKESPEKAQAQAAAAQATERKAKIRRQMLTVHQAFGFTTLAALAATLVI
                     GQLNYQDQYVNGQFSGRFERPHLGLGIGTTALFATTGALALFSPDPYEKKYRLDTAMV
                     HRVSMALATAGMVTQMILGPITSFRVGYTNQSRLALGHLITGYATFAFMATGTIAYFF
                     "
     gene            3845..>4180
                     /locus_tag="PKL17_20800"
     CDS             3845..>4180
                     /locus_tag="PKL17_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47234.1"
                     /translation="MTKRTQLSASTFRLFAFGLLCGSALLPSGGCETDSATYLDPLDM
                     AVAQLPDASNPATGLPCDVSKVLASNCLACHATAQGPSKLALATYDDLVAASRSNPAK
                     KVVEVSVQRM"
BASE COUNT          787 a         1396 c         1229 g          768 t
ORIGIN      
        1 ccgcggtcgc gaccgatttt tccgcaggtg gctgcggcgg gacagaagat gtgaccgtcg
       61 gcagtcgggc ttgccagcgc tcgccgtcgc ccaaaatcag gctgcgattg ctgtgtggac
      121 ggacttcgac gcggccatgc acaacccgca ccgaaaccag gtgatcttcg acgacttccg
      181 tctcgaacgc cgtgccacgc acttcgacct ctccgtcggg agcgatgacg cgaaagcgct
      241 cgcccttgcg cagcggtgcg acttcgatga gcagcttgcc gttcgacagc cgcacccact
      301 cgtcgccctg ctttcgttcg tggacaaaat gcacgtcccc gccttgcgtc accacggtgg
      361 cgcggaaacc tgctgcatcc gacgatgggt gacgctcgcc gccccacaac cgaaacagca
      421 aaatcagaca ggcggcggcg cttacggcgg cgctcagggc caggtaacgc agcggcacca
      481 tcttggtcgt gctcgtcggt ggcgattcca gcttggccag aagctgcgcc ttttgcgaat
      541 ccagctctgc tgcatcggga acgtagctgg gaagctgttt tccgagcgca cgcagctcga
      601 cgagcgcact ccattcggaa gagcacgacg gacactgctc gatgtgacgg agcaggcctg
      661 gttcagcgct ttccaaaaag gcgcgaaata gctctgcttg cgacggacag ctcgcggctc
      721 tcatggggtt cctcttgcag cttttgccaa agccgctggc gcgcttcgta aagccttcgc
      781 cacagcgttc cgtcgggaat tcccagcgcc ttggccactt cggcgccagg gagttcctcg
      841 acgtagacca tcaaaaaggg aatgcgaagc tcggcaggaa gcgtggccac cgctgcgtca
      901 agacgcgcaa tttgttcgcg ctgcgcggtg ttgcgttcgg ggctgttcgg ctctgcgacg
      961 ggtttttgcg aaaaacgcac cgccatctgt cgccgtcgcc cttcgccccg tcggaaatgt
     1021 ctgccgacgt tggctgcgac tgcgtacacc caggtctggg ccgacgacgc gccacgaaac
     1081 gccgccgccg aacgccagac ttccaaaaac gtcatctgca ccaggtcgtc tagctctggc
     1141 gtgtcagcgc cgcacagcct cgacaggaag cgatagaccc cagggcttaa ccgttcaaac
     1201 aaaagaccga gcgcgccacg atcccctcgg ccacacgcag cgacgaggtc ttcgtttgtg
     1261 cgttccgaaa tggttccgcc gccagcggca aaatgcagca gcttgcccgg catttacaga
     1321 ctccacctga ggcccagacc gccccgcaga agtccccacg ccgtcgtagg aaccgccaac
     1381 gtgcgctttg tccccagtcg aaccagcagg ttgccgctat ccagaaacgc tccatctgcc
     1441 gccagcatca cggaaagcag caggttgcga tacagcgcca ccgccgtcga aaaacgagcc
     1501 tcggcacgca gtccgccacc ggtgatggag agcggtcccg aaagcagccc ttcatcttcc
     1561 tcctccgagt gcggcgcccc cggattcaga cgaaattcac ttcgcaccat gcccgcaccg
     1621 acgccaatcc ccggtgcaaa cgtgaagcga ccgaccgtca ctggcagctc ggcgtcgacc
     1681 agcaagtccg ccgacaggcg cgacagcagc gtttcgatac tgacgaaccg cggcagatcg
     1741 cctgccatcc gcagcgtcgt gccaagacac agccgtccga cgtacagaca gccatgaaac
     1801 cgcccgccaa accacgacgc cgacttgagg tcgatccccg cctccggtgc caattccagc
     1861 gtcgcgcgcg gcagcgtcgg aggcagcggc tcgggcacag gtgacaacgg ctgctcgggt
     1921 tcgggcatgg gcggcgccaa catcgcagac agaagcggcg ccgagatgtc actgcgcagc
     1981 catgtttcga tcagcgccac cgccgttgcg acgtctgcca ccagccgttc ttcgcgcggg
     2041 acatcttgct gcaggaccag cttgaacagg ccgccttttt gcgacacttc ggcccgcagc
     2101 gtcggacaac ctggcacaac ggtggtcgct atcgcccgcc gcgaaagtgc atcggccaag
     2161 ctccgatgaa gccctggttc cccgacgatc tgcacctgcg gtgggcagtt cgcggcggtt
     2221 ggcagcgacg gcaccggatc actttgcagc gccggttcgc tggccgtcac caccgaaagc
     2281 cgcagggtca gccaaagccc agccagacaa agccgcgtcc gccttctgca tggtcgaaaa
     2341 gcgcgctgcc ctgccggact gtgtttcacg aagcggaccc ctttggtgcc aagcctgcgt
     2401 cgctagttca ggtaaatgcg cccgcccgcg gcgccgcccg gacggtggca cgaagcacag
     2461 ttgcccgtcg tcaccggctg cgtcatttct ttgactttgc cgtctttgct gacgcgcgcg
     2521 atcatcgttt ggtagagcag cttttcggcg gtgaaaaagt tgcccgcgcg gttcgtcgtc
     2581 agcgtgatga ggactttgcg cgtttcgtcg gccagctcga ctttgacgcc ttcgagcccg
     2641 ccggtattgc aggtgctgct accgcttgca tagacggttc cgcccgcggt ccacggcaag
     2701 tcgcctgcct gcgaaccggc cacatggcat gccaggcagt cccgtccggg cagcatcggc
     2761 gcttgcggca ccaagttgtc ttccgacggc gcttcacagc ccgccgccgc ccccggcacc
     2821 gcatcgacgt tgacttccga gcaccccgaa aaccacagca aaagcggcag caacaggccc
     2881 caccgcgcct tgttcaaaac cagagaggat cgaaacagtg acatgggttt gttcctctct
     2941 tcctaaaaga aataggcgat ggtgcccgtc gccataaacg cgaacgtggc atagcccgtg
     3001 atgaggtgtc cgagcgccag cctggactgg tttgtgtaac cgacgcggaa cgacgtgatc
     3061 gggcccaaaa tcatctgcgt gaccatgccc gccgtcgcca gcgccatcga aacccggtgc
     3121 accatcgcgg tgtcgagccg gtatttcttt tcgtaggggt cgggtgaaaa cagcgccagc
     3181 gcgcccgtcg tggcaaacag cgccgtcgtc ccaatcccca gtcccaggtg gggccgttca
     3241 aaccgcccgg aaaactgccc gtttacgtac tggtcttggt agttcagctg cccaatcacc
     3301 agcgtcgcag ccagcgcagc cagcgtcgta aaaccgaacg cttggtgcac cgtcagcatc
     3361 tgccggcgaa tcttcgcttt gcgctcggtc gcttgcgcgg cggcggcttg ggcttgcgct
     3421 ttttccggcg attctttcgg accgtcatcg agcaagttaa acgaaagatc gtcgctaccc
     3481 gtcgctgggg ccgtcgttgc tggggccgtc gttgctgggg ccgtcgttgc tggggccgtc
     3541 gtcgctggcg ccggtggcga ttccgcgttt gctgtcgaat agctcgcgat ccaggccaag
     3601 caggttagga ggcaaacaca ccgaaggttt ggtttgggca tcaaaaactc cgcaaattgt
     3661 ccgcagtcat cctcgcagca ggaaccgagc cggatcgtat ctttttgtgc ccgagatggc
     3721 gccgcccttc gcgacttgtt ttttgttttt ttgaagagcc gctttgctgt gggcactaca
     3781 catagggagt cagacagaga cctacggaca cccaccggaa cacaagctcg ttgaggatgc
     3841 gctgatgaca aaaagaaccc aactttccgc ttccacattt cggctgtttg cattcggcct
     3901 cctgtgtggg agtgccctcc ttccttccgg cggctgcgaa accgacagcg ccacgtatct
     3961 ggacccgctc gacatggcgg ttgcgcagct gcctgatgcc agcaatcctg cgaccggtct
     4021 gccctgcgat gtcagcaaag tcctcgcgtc caactgcctg gcttgccatg ccaccgccca
     4081 aggtccgtcg aaactggcgc tcgccaccta cgatgacttg gtcgcggcat cgagatcgaa
     4141 tccagcgaaa aaagtcgtcg aggtatcggt gcagcgcatg
//
LOCUS       DAORGN010000306         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12038_length_4074_cov_5.179149, whole genome shotgun sequence.
ACCESSION   DAORGN010000306 DAORGN010000000
VERSION     DAORGN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12038_length_4074_cov_5.179149"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKL17_20805"
     CDS             <1..404
                     /locus_tag="PKL17_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47235.1"
                     /translation="TSVLVIPANARYTGATGIFRSVWASDPSNVFVVGSDYGTVGTPM
                     LKWDQSAGKWTDFNPITSQVTSQALLGVWGRSPSDVYAVGKDGMVLHFNGNVWNFEQS
                     GTENNLVSVTGNASQVYITGVGGSILKKTLR"
     gene            410..2572
                     /locus_tag="PKL17_20810"
     CDS             410..2572
                     /locus_tag="PKL17_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47236.1"
                     /translation="MNSLSSRRGGLLAGALLLSLSVSACEETPVPKGPLRVPSSSPDQ
                     GGPQFVSTKQTFEDAEQAQLVLDLVQKWGEPQRPRIERGVRQVAALWQKTDGDLAAFV
                     REQFVSDEKQLDALVTRMQEVFEQIDGHNNEIQRELRRPLDTDSGPLGPLDTQLGLVD
                     PSSHVIEDLFRSKVGFVVLLNLPLTTLEERLSAGDKWSRRKWAEVRLVQRFARRIPPS
                     VQQQVTESVVAGERYIAGYNLYLHHLLDEKGQRLWPKGKRLISHWNLRDEIRADYAEP
                     DGMSKQETIRKAMERIVEQSIPQVVIDNPRVDWNPFTNQVSLSPPDEIEPSDQKTPLP
                     PADKLGEREPDRRYALLLAQAQAQRLVDPYSPTEKTLIDRSFQTSREIPEAETVRMLV
                     EVCSSPLIKELAQRAEKQLGRKLVPHDLWYAGFRPRGQFTESALDEVTRKRYPTAASF
                     EADLPNILTKLSFAKDKANFVAQHIRVDASRGAGHAMQAMRRGDFPRLRTRVGPGGMD
                     YKGYNIAVHELGHNVEQVFSLYEVGDTLLAGVPNNAFTEALAFVFQMRDISLLGLAKP
                     DPEADRRRILSDFWDTWEIAGVALVDIAAWHYMYDHPQATAKELRAEVVRIAKETWNR
                     YYADVLGGKDVLLLGVYSHMIQNTLYLPDYPIGHLIAAQIEEHLAKQPPGSLGSEFER
                     MAKMGAVTPDLWLQHATGAKLSTAPLLRMTQAALAASK"
     gene            2621..>4074
                     /locus_tag="PKL17_20815"
     CDS             2621..>4074
                     /locus_tag="PKL17_20815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNK47237.1"
                     /translation="MFERRSRASLLNLHLAWAASLSIGGTAYAQASPDVAQPAAADPA
                     AASELVGVVREQGTRDPLPGIKVTLRRSADDKSPLVVETDDEGRFRFAKLLPGPVLIE
                     LFASRIRKTTGQETLPNGRLEVVYFAPPKGNPFEVVVKAAPLRREVVSTTLSVTELKK
                     IPGGQNDPIKAIQNLPGVARAPFGAGQIVVWGSAPGDTRVYADGVQIPRVYHFGGIRS
                     TISAEFVTDVNFRPGAYGADYGRGLGGVIEVATRRPKSDRWHGSVTLDLIDGNVTVEG
                     PVTKNLHVALGGRVSWVSAFLPLFNSSGTQLSPFYWDYQFSAHYRASERDDLDLFVFG
                     ATSNITAKVDDPDPNSVIHIDSKSYFGRARLRYTHRFSKDSVFWLSPSLGGDTFTVET
                     GAQGLDGQPVNLRVLQLGYNLRSELRQKWGSHIDYALGLDFEGVRARVETVMPLAPGA
                     GDSQGLPVGGADMVKDAGRSENGFFGDAFFSDSNG"
BASE COUNT          802 a         1189 c         1312 g          771 t
ORIGIN      
        1 gcacgtcggt gctggtgatc ccggccaacg ctcgttatac cggcgcgact ggtatcttcc
       61 gcagcgtctg ggcgtcggac ccaagcaacg tgtttgtcgt cggcagcgac tacggcaccg
      121 tcggtacgcc gatgctcaaa tgggatcaaa gcgcggggaa atggaccgac tttaacccga
      181 tcacgtcgca ggtgacgtcg caggcgctgc tcggcgtgtg gggtcgcagc ccatcggacg
      241 tctacgcagt cggcaaagac ggcatggtgc ttcactttaa cggcaacgtc tggaacttcg
      301 agcagagcgg caccgaaaac aacctcgtca gcgtcaccgg caacgccagc caggtctaca
      361 tcacgggcgt cggtggcagc atcctcaaaa agaccctccg ctaagcttca tgaacagcct
      421 ttcctctcgc cgcggcgggc tccttgcggg ggccctgctt ctgtcccttt ctgtgtcagc
      481 ttgtgaagaa acgccggtcc ccaaaggtcc gctgcgggtt ccgtcgagca gccccgatca
      541 gggtggcccg cagttcgttt cgaccaagca gacgttcgaa gatgccgagc aggcgcagct
      601 cgtgttggac ttggtccaaa aatggggcga accccagcgt ccgcgcatcg agcggggcgt
      661 gcgccaggtg gcggcgctgt ggcaaaaaac cgacggtgat ctggccgcct ttgtccgtga
      721 gcagttcgtg agcgatgaaa aacagctcga cgcgctggtc acccggatgc aggaggtctt
      781 cgagcaaatc gacggtcaca acaacgaaat ccagcgcgag ctgcggcgac cgctcgacac
      841 cgattctgga ccgcttgggc cgctcgatac gcagcttggc ttggtcgatc cgtcgtcgca
      901 tgtgatcgaa gacttgtttc gtagcaaagt cggttttgtc gttcttttga acctgccgct
      961 gacgacgcta gaggagcggc tttccgccgg agacaagtgg tcgcgacgca agtgggccga
     1021 agtgcggctt gtgcagcgtt ttgcgcggcg gattccgcct tctgtgcagc agcaagtcac
     1081 cgaatcggtc gtcgcgggcg agcgctacat cgccggctac aacctgtatc tgcatcatct
     1141 gctcgatgaa aaagggcagc gcctttggcc caaaggcaag cggttgatca gccactggaa
     1201 cctgcgcgac gaaattcgtg ccgactatgc cgaacccgac ggcatgagca agcaggagac
     1261 gattcgcaaa gcgatggagc gaattgtcga gcagagcatt ccgcaggtcg tcatcgacaa
     1321 tccgcgcgtc gactggaatc cgtttacaaa ccaagtctcg ctgtcgccgc ccgatgagat
     1381 tgaaccaagc gaccagaaga cgccgctgcc gccagccgac aagctgggcg agcgcgaacc
     1441 ggatcgtcgc tatgctctgc ttttggcaca agcgcaggcg cagcgactgg tcgatccgta
     1501 ttctccgacg gaaaaaacgc tgattgatcg cagctttcag acctcgcggg aaattccgga
     1561 agccgaaacg gtgcgcatgc tcgtcgaggt gtgcagctcg ccgcttatca aagagcttgc
     1621 gcagcgtgcg gaaaaacagc ttgggcgcaa gcttgtgccg cacgacctgt ggtacgccgg
     1681 tttccgtccg cgcggccagt ttacagaatc cgcactcgac gaagtgacgc gcaagcgcta
     1741 tccgacggcg gcgtcttttg aagcggatct gccaaacatc ctgaccaagc tgtcgtttgc
     1801 caaagacaaa gcaaacttcg tcgcccagca catccgcgtc gatgcgtcgc gcggggccgg
     1861 gcacgcgatg caggccatgc gtcggggcga ctttccgcgg ctgcgcaccc gcgtcggtcc
     1921 tggcggaatg gactataaag gctataacat cgccgtccac gagctgggtc acaacgtcga
     1981 gcaggtgttt tcgctgtacg aagtcggtga cacgctgctt gcaggggtgc caaataacgc
     2041 atttaccgag gcgctggcgt ttgtgttcca gatgcgcgac atctcgcttt tggggctggc
     2101 caagccggac cccgaggcgg accgtcggcg gattctttcc gatttttggg acacctggga
     2161 aattgccggc gtggcgctgg tcgatatcgc ggcgtggcac tacatgtacg atcacccgca
     2221 ggcgacggcg aaagagctgc gggccgaggt cgtgcgaatc gccaaagaga cgtggaatcg
     2281 ctactacgcc gacgtgctgg gtggcaaaga cgtgctcttg ctcggcgtgt attcgcacat
     2341 gatccaaaac acgctgtatc tgccggacta tccgattggt catctgatcg cggcgcagat
     2401 cgaagaacat ctggccaaac agccgccggg cagcctcgga agcgagttcg agcggatggc
     2461 gaaaatgggc gcagtgacgc ccgatctgtg gctgcaacat gcgacgggtg ccaagctgtc
     2521 gacggcgcca ctgctgcgga tgacccaagc ggcgcttgcc gcgagcaaat agcccacctg
     2581 cagtcgtgta aaaaaacgaa aaattcgcta cgttgcgcgc atgttcgagc ggcggtcgcg
     2641 tgcgagcttg ctgaacctgc acttggcatg ggcggcgtcg ctttcgatcg ggggaacggc
     2701 ctatgcacaa gcttcgcccg atgtggcaca gccggcggca gcggacccag cggcggcaag
     2761 cgagctggtt ggtgtggtgc gcgagcaggg aacccgcgat ccactgcccg gcatcaaggt
     2821 cacgctgcga cggtcggctg atgacaaaag cccgcttgtg gtcgaaaccg acgacgaagg
     2881 ccgctttcgt tttgccaagc tgttgcccgg tccggtgctg atcgagctct tcgcatcgcg
     2941 gattcgaaag accacagggc aagaaacgct gcccaatggc cggctcgaag tggtctattt
     3001 tgcgccgccc aaaggcaatc cgttcgaggt ggtggtcaaa gccgcgccgc tgcgtcgcga
     3061 agtggtgtcg acgacgcttt cggtgacgga gctaaagaaa attcccggcg gccaaaacga
     3121 tccgatcaaa gcgattcaaa acctgccggg ggtggcgcgg gcgccgtttg gagccggcca
     3181 aatcgtcgtg tggggatcgg caccgggcga tacgcgggtc tatgccgacg gggtgcagat
     3241 tccgcgcgtc tatcactttg gcgggattcg ttcgacgatc agcgccgagt ttgtcaccga
     3301 cgtaaacttt cgaccggggg cgtatggcgc cgactacggg cgcgggcttg gcggagtgat
     3361 tgaagtcgcg acgcggcggc ccaaaagtga tcgctggcac ggatcggtca cgctggacct
     3421 tatcgacggc aatgtgacag tggaagggcc agtgacaaaa aacctgcacg tcgcgctggg
     3481 cgggcgggtg tcgtgggttt cggcgttttt gccgcttttt aacagcagcg gcacgcagct
     3541 ttcgccgttc tattgggact atcagttttc ggcgcactac cgggcgagcg agcgcgacga
     3601 cctggatctg tttgtctttg gcgcgacgag caacatcacc gccaaagtcg acgaccccga
     3661 tcccaacagc gtcatccaca tcgacagcaa gtcgtatttt ggccgggccc ggctgcgcta
     3721 tacgcatcgc ttttccaaag actcggtgtt ttggctttcg ccatctttgg gcggcgatac
     3781 cttcacggtc gagactgggg cgcaagggct cgacggacag ccggtgaacc tgcgcgtgct
     3841 ccagcttggc tacaacctgc gcagcgagct gcgacagaag tggggctcgc acatcgacta
     3901 tgcgctcggg ctcgattttg aaggggtgcg ggcgcgggtg gaaacggtga tgccgctggc
     3961 acctggcgcg ggcgacagcc aaggcctgcc cgtcggtggc gccgacatgg tgaaagatgc
     4021 cgggcggtcg gaaaatggct tttttggcga tgcgttcttt tccgattcca acgg
//
LOCUS       DAORGN010000307         4062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12098_length_4062_cov_6.163714, whole genome shotgun sequence.
ACCESSION   DAORGN010000307 DAORGN010000000
VERSION     DAORGN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12098_length_4062_cov_6.163714"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..256)
                     /locus_tag="PKL17_20820"
     CDS             complement(104..256)
                     /locus_tag="PKL17_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47238.1"
                     /translation="MPRTKRKLKNRAKNHVRHVTKATKRRTFAAGGNRCANTRRARKL
                     RKLAAK"
     gene            391..3951
                     /locus_tag="PKL17_20825"
     CDS             391..3951
                     /locus_tag="PKL17_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47239.1"
                     /translation="MANLPVVELLPASRMIEKLAAVVHDERIRMMVSLGVLSRRDPKA
                     KQLLWELWQGPVFARRLVLKSCQGSLDGDLVMAGLADSSRLVRGLAGKLVARCCTDEQ
                     ATQALKSAFAAHRHLPLSLQLKRAKRTEPIDRFVDFLVAQGQSFYLCDVVPLGSEASA
                     RRHLHKALAQPSSAFFHRLVLCHPQLFAESAAVAMQKGPLTGPWKVAVSQELHTLAVK
                     TPDAALWLIDALLSNGEAFPTTLFSVLAERRPGPLVDLCAKHKIKLPSGLYQHRANKL
                     SPPHFRYLVAECRLALGNPRTFFRKLGAAEKEAVIQTFESSKLCEPSWGAGLLAALPR
                     DSVVREPIYKAWSAASQNPDGVIPREVLWHLPKDLQEREARRHLVDVTALRTRPEQRI
                     LYATLLPWDEAAPHLKAYLGHPEGEMRGQALVALLGIPGRLPPEPALVEEALRICVGK
                     KFEQDPVRLRMFQALAGWPKAVWDKKYLPQVGQLLRDALDAADLSHATAQAAERLLVR
                     LFFLDGEFGGKWLATLLKERGTIYAPGLGNMLSDDEVRALAKPLLSVAESWSSREREA
                     QLWALTQSLGHRIALVDGLPELLEKVMERTTQATVSFGILHLFYLHQRARFDRCVAQV
                     VKVWVDKGWDAQLAYLIRAVDKIPASVEDSVEKALLRTTYPHQAELLLSAVKNAHSAL
                     LERLVPKALKTDRSFICVPTVWMYLHKRRQDLLTPYLAGEVVRGRFATGKTRWVLPFH
                     DGFYRWSSEQQILFTHTLGGLYGDDDRDTPTILWAMERLCGLAYTSGESLIGLCDDKR
                     PAVSERAVRTLSRLDAGQGVPKLLACLEDQRARFAIYGLRKALFEMPIAQAVAILKTV
                     PLKKVTVAKEVVRLCGELRSDEAYKLLLSLDRPDLHRDIRIALCRALWDHLDRDESWQ
                     VLTQAATGPDFILATRVGDIPAQRLTQKTDAKLAQVLASVLLRPEPEARRELLLKAPS
                     LPLHDQSRLFLSAILDRLDSRYYDEVEAAVQAVFLRALPTDVPAYAAQIDKTRKNRRA
                     LAVIVDQLGAKFDRKSQLMVSLGGETLRVLAKEAALSSLLLRAMVKLGQADELATLLV
                     DLADQDRLSRESIAAAEAAVQALPGDDLEGLELRLAASRHPALRLLAVRALQAAAVKG
                     QGFTDARRQRLRVYQQDPAVEVAAAAQLVFPPLSETENAS"
BASE COUNT          745 a         1134 c         1389 g          794 t
ORIGIN      
        1 gcatggagat gacgcagcga tggaatgcgg cgaaatgaag ctgccgcgca gcgggaagcg
       61 ggtgggatgg aaaagtagcg aaagagcaga agccgctggg ccgttacttg gcggcgagct
      121 tgcgcagctt gcgggcgcgg cgggtgttgg cgcagcgatt gccaccagca gcgaaggtgc
      181 gacgcttggt ggctttcgtg acgtgacgaa cgtggttttt ggcgcggttt ttgagtttgc
      241 gcttggtacg gggcattgca aaagtcctct ctagaacgtc aggtgacagc tatcgaatcc
      301 gcagcctact cgacttgccc cctgcccgaa aaggcccaaa ttccgagtat cacaagataa
      361 aatggtcccg gcccaaacgg gggggacaag atggccaatc tgcctgtggt cgagcttttg
      421 ccagcgtcgc gcatgatcga aaagctggcg gcggtggtgc acgacgagcg cattcgcatg
      481 atggtgtcgc tgggggtttt gtcacggcgc gatcccaaag cgaaacagct tctttgggag
      541 ctatggcaag gtccggtgtt tgcgcggcgg cttgtgctca agagctgtca gggcagcctt
      601 gatggcgact tggtgatggc ggggcttgcg gactcatcac ggctggtccg ggggctggct
      661 ggcaagctgg tcgcacgctg ctgtaccgat gaacaagcga cgcaggcgct caagagtgcg
      721 tttgccgccc atcgacacct gccgctttcg ctacagctca agcgggccaa aaggacggag
      781 ccgatcgatc gcttcgtcga ctttctggtt gcccagggac agtcgtttta cctgtgcgat
      841 gtggtgccgc tcggcagtga agcgagcgcg cgcagacacc ttcacaaagc gctggcccag
      901 ccgtcgagtg cgttttttca ccgcttggtg ctgtgtcatc cgcagctttt cgccgagtct
      961 gccgcggtcg ccatgcaaaa ggggccgctt accgggccgt ggaaagtcgc ggtttcgcag
     1021 gagcttcaca ccttggcggt gaagaccccg gacgctgcgc tttggttgat tgacgcgctg
     1081 ctttcaaacg gggaagcgtt tccaacgaca ctgttttccg tgctggccga gcggcgacca
     1141 gggccgcttg tcgacttgtg tgcgaagcac aagatcaagc taccgagcgg actttaccaa
     1201 catcgcgcaa acaagctgtc gcctccgcac tttcgctatt tggtcgcgga gtgtcgactt
     1261 gcgctgggca atccgcggac gttctttcgc aagctgggcg cggcggaaaa agaagcggtc
     1321 atccagacat tcgaatcgag caagctgtgc gagccatcgt ggggcgcagg acttttggct
     1381 gcgctgccgc gggattcggt ggtgcgtgag ccgatctaca aggcctggtc ggcagcgagc
     1441 cagaacccag acggcgtgat ccctcgcgaa gtgctgtggc atctgcccaa ggatctgcaa
     1501 gagcgcgagg cgaggcgaca tctggtcgat gtgacggcgc ttaggacgcg gcccgagcag
     1561 cgcattttgt acgcgacgct tctgccgtgg gatgaagcgg caccgcattt gaaagcgtat
     1621 ttaggacatc ccgaaggaga gatgcgaggt caggcgctgg ttgcgctgct cgggattccg
     1681 gggcgactgc cgcccgaacc ggcgcttgtc gaggaagcgc tgcgcatctg tgtcggcaaa
     1741 aagttcgagc aagatccggt gcggctgcga atgtttcagg cgctggctgg ctggcccaag
     1801 gcggtctggg acaaaaaata cctgccgcaa gtcggacagc ttctgcgcga tgcactggat
     1861 gcggcggact tgtcacatgc gacggcgcag gcggcagagc gactgcttgt gcggctgttt
     1921 ttcctcgatg gggagtttgg cggcaagtgg ctggccacgc tgctcaagga acgcgggacg
     1981 atttacgcac cgggacttgg caacatgcta agcgacgacg aagtgcgtgc cctggccaag
     2041 ccacttcttt cggtcgccga gtcgtggtcg tcacgcgaac gagaagccca gctctgggca
     2101 ctgacgcaga gcttgggcca tcgcatcgca ctggtcgatg ggctgccgga gctgctcgaa
     2161 aaggtgatgg agcgcacgac ccaagcgacg gtgagctttg gcatcctgca cctgttttat
     2221 ctgcaccaaa gggcgcgatt cgatcgctgc gtcgcccaag tggtcaaagt gtgggtcgac
     2281 aaaggctggg atgcacagct tgcgtatctg atccgcgccg tcgacaaaat tcctgcaagc
     2341 gtcgaagatt cggtggaaaa agcgctgctg cgcacgacgt acccgcatca ggcggagctg
     2401 ctcctttcgg cggtgaaaaa tgcgcactcg gcgctgctag agcggcttgt ccccaaggcg
     2461 ctgaagacgg accgcagctt catttgtgtt ccgacggtgt ggatgtatct gcacaagcgg
     2521 cgacaggatc ttttgacgcc gtacctggcc ggtgaagtgg tgcgcggacg ttttgcgacc
     2581 ggcaagacgc gctgggtgct gccgttccat gacggcttct atcgctggag cagcgagcag
     2641 cagattttgt ttacccacac cttgggcgga ctgtatggag acgatgaccg cgacacacca
     2701 accatcctgt gggcgatgga aaggctgtgc ggcttggcgt atacgtcggg cgagtcgctg
     2761 atcgggctgt gtgacgacaa gcggcctgcg gtgagcgagc gagcggtgcg aacgctttcg
     2821 cggctcgatg cggggcaggg cgtgcccaag cttctggcct gtctggaaga ccagcgggcg
     2881 cggtttgcga tttatggact gcgcaaagca ctgttcgaga tgccgattgc gcaagcggtg
     2941 gcgattctga agacagtgcc gctcaaaaaa gtcacggtgg ccaaagaagt cgtgcggctg
     3001 tgcggcgagc tgcggagtga cgaagcgtac aagctgcttc tgtcgctgga ccgtccagac
     3061 ctgcaccgcg acattcgcat cgcgctgtgc cgggcgctgt gggatcacct ggaccgcgac
     3121 gaaagctggc aggtgctcac gcaggctgcg accggaccgg acttcatctt ggccacgcgg
     3181 gtcggtgaca ttccggccca gcgactgacg cagaagaccg atgccaagct ggcgcaggtg
     3241 ctggcgagcg tactgcttcg tcccgaaccc gaggcgcggc gcgagcttct tctgaaagcg
     3301 ccgagcttgc cgctgcatga ccaaagccgg ctgtttctgt cggcgatttt ggaccggctc
     3361 gattcgcgct actacgacga ggtcgaggca gccgtgcagg cggtgttttt gcgggcgctg
     3421 cccaccgacg tgccggccta tgcggcgcag attgacaaga cgcgcaaaaa ccgacgggcg
     3481 ttggcggtga tcgtcgatca gctcggggca aagttcgatc gcaaaagcca gctcatggtt
     3541 tcgctcggtg gtgagacgct gcgggtgctg gccaaagaag cggcgctgtc atcgcttttg
     3601 ctgcgggcga tggtcaagct gggacaggcc gatgaactgg cgacgctgct tgtcgacttg
     3661 gcggaccagg acaggctttc gcgtgagtcg attgcggcgg cggaagcggc ggtgcaggcg
     3721 ctgcccggtg acgatctcga agggctggag ctgcgacttg cggcgagccg gcatccggcg
     3781 ctgcggcttt tggcggtgcg agcgctgcaa gctgccgcgg tcaaaggcca aggctttacc
     3841 gatgcccggc gacagcgtct gcgtgtttat cagcaagatc ccgcggtcga agtggccgcg
     3901 gcggcccagc tcgtcttccc accactgtcc gaaacagaaa acgccagcta ggctcggcag
     3961 cgcgccttca ccgcttggcg ccgcgctgtt acttgagatt gccgtaaggg tcggcagagc
     4021 tttcgccgcc cgattcatcc ccgctgctgt ctttcggttt gt
//
LOCUS       DAORGN010000308         4056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12131_length_4056_cov_6.526868, whole genome shotgun sequence.
ACCESSION   DAORGN010000308 DAORGN010000000
VERSION     DAORGN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12131_length_4056_cov_6.526868"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PKL17_20830"
     CDS             <1..793
                     /locus_tag="PKL17_20830"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNK47240.1"
                     /translation="GASLEALWLDADQKTVTVQVAGEVHRVDYLATQNLLRELARSAR
                     REAVARKPDPPGLSPSLRWEYLYHHGGDGWEMGKATPPLARYFRAHPPAAATRSLVVG
                     CGRGHEAILLAEVGDPSARVIGIDLAPAAVQIASRLAAQKGVSDRVSFAQQDLFAWPS
                     REPSECGSFDLVVEHNCFCAIEPQHRDDYVAAVAQLLRPGGRLVGLFYPHDYPGGPPY
                     ASTADEIQRRLQAAFSITYQETPTDSAITRAGLELLLVAERRASG"
     gene            819..1985
                     /locus_tag="PKL17_20835"
     CDS             819..1985
                     /locus_tag="PKL17_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK47241.1"
                     /translation="MPFTGVDYFQITDLLSEEERLIVSTFRRFVDDEIMPVIGRHFRD
                     GTFDRNWPKQLGALGALGANLVGYGCPGLNAVAYGLIMQELERGDSGIRSFASVQGAL
                     VMYPIHAFGSEEQKQRFLPKLATGELIGCFGLTEPDFGSNPGGMRTTAKETADGYVLN
                     GEKRWITSGGLADLAVVWAKCDDGVVRGFVVERGLKGFSTRDIHGKYSLRASVTSELI
                     FEDCLVPKSALLPGVKGLRGPLSCLNQARFGIAFGAVGAAMACYDEALSYTKDRVQFA
                     GKPIASHQLVQLKLVDMLQEITKAQLLGLQLGRLKEAGKVRPQQVSLAKRNNVKMALE
                     CARNARDLLGANGITDEYQSGRHMCNLESVFTYEGTDHIHTLIVGEDITGCSAF"
     gene            complement(2203..2514)
                     /locus_tag="PKL17_20840"
     CDS             complement(2203..2514)
                     /locus_tag="PKL17_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47242.1"
                     /translation="MHDTTRSTQAARNWRIRALLPFATVLAIGCGGGAPEASPALLSG
                     MPRCDVSLLHPHIAVGSVAGQPGKTIVYVDGVMACVDDSSRVDQILTQLEVKNPGILA
                     R"
     gene            complement(2576..3229)
                     /gene="infC"
                     /locus_tag="PKL17_20845"
     CDS             complement(2576..3229)
                     /gene="infC"
                     /locus_tag="PKL17_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNK47243.1"
                     /translation="MRPESRPSPSEQYRVNHRIRIPRVRVIGSDGQQIGILETPDALR
                     MAREEGLDLVEISPKAVPPVCKIMDYGRFKYETAKKEREERAKRTVIEVKEMKFRPKT
                     DEHDFDFKVKHIREFLAEGNKAKLVIQFRGREIVHPEVGQNVLRRVFEACQDVGIVEQ
                     PAMMEGRRMLMIIGPRPQTVQKPKPATPAAPAPGTSAAPAAPAPMTAAAAPSSKDER"
     gene            3425..>4056
                     /locus_tag="PKL17_20850"
     CDS             3425..>4056
                     /locus_tag="PKL17_20850"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNK47244.1"
                     /translation="MTLNIWGNHAPWSDRRRLIRAELLRLDADVVALQEVLRPLGPGT
                     SQADELAVDLALPAPYRVCFGKACQIDKPFPAEFGNAALIRSPLREQRVVKLPTPEGI
                     ETRSALYILLSVKVGLLPVVITHLSWEPELSETRAAQLRHIQQFLTGEMAALPTRVPP
                     HVPILPPLLLGDLNAPPGSAEIQVLTAPAPGTLAFVDGFAQVGAGPAETFS"
BASE COUNT          695 a         1269 c         1259 g          833 t
ORIGIN      
        1 aggcgcgtcg ctcgaagcgc tgtggctcga cgcggaccaa aagactgtca ccgtgcaggt
       61 tgccggtgag gtccatcggg tcgattatct ggcgacgcag aacttgctgc gtgagctggc
      121 ccgctcggcg cgtcgcgaag ccgtggccag aaagcccgat ccgcccggac tttcgcccag
      181 cttgcgctgg gagtacctgt atcaccacgg cggtgacggc tgggaaatgg gcaaggcgac
      241 gccgccactc gcgcggtact ttcgcgccca tccaccggca gcagcgacgc ggtccctggt
      301 ggtcggctgt ggccgcggtc acgaagccat cctgcttgcg gaagtcggcg atccatcggc
      361 gcgcgtcatc ggcatcgacc tggcccccgc ggcggtgcag attgcgtcac ggctggctgc
      421 ccaaaaaggc gtgtccgatc gcgtttcttt tgcacagcag gacttgtttg cctggccaag
      481 ccgcgaaccc agcgagtgcg gaagcttcga tttggtcgtc gagcacaact gcttctgcgc
      541 catcgaaccg cagcatcgcg atgactatgt ggccgcggtt gcccagcttt tgcgtccggg
      601 cgggcggctc gttggtctgt tttatcccca cgactatccg ggcggaccgc cgtatgcctc
      661 gacggcagat gagatccagc gccggctaca agccgctttt tccatcacct atcaagaaac
      721 cccgacggac tctgcgatca ctcgcgccgg tcttgagctt ttgcttgttg ccgagcggcg
      781 tgcgtccggc tgaccgtacc cccgaaaagg agctgcccgt gcctttcacc ggcgtcgatt
      841 atttccaaat caccgatctg ctcagcgagg aagaacggct catcgtttcg accttccggc
      901 gttttgtcga cgatgagatc atgccagtga tcggtcggca ctttcgcgac ggtacgttcg
      961 atcgcaactg gcccaaacag cttggggcgc tcggggcgct cggcgcaaac ttggtcggct
     1021 acggctgtcc ggggctcaac gccgtcgcct atggcctgat catgcaggaa ctcgagcgcg
     1081 gcgactcggg catccgcagc tttgcctcgg tccagggcgc gctggtcatg tatccgattc
     1141 acgccttcgg ctccgaagag cagaagcagc gttttttgcc caagctggcg acgggcgagc
     1201 tgattggctg ctttggtctg accgaaccgg actttggttc caatcccggc ggcatgcgca
     1261 ccaccgccaa ggaaaccgcg gacgggtacg tgctaaacgg cgaaaagcgc tggatcacat
     1321 ccggcggtct ggccgatctg gctgtggtct gggccaagtg tgacgatggc gtggttcgtg
     1381 gttttgtcgt cgaacgtggc ctcaagggct tttcgacccg tgacatccac ggcaagtatt
     1441 cgctgcgcgc ctcggtaaca tctgagctga tctttgaaga ctgcctggtt cccaagtcgg
     1501 cacttttgcc cggtgtcaaa ggtctgcggg gcccgctttc gtgtctgaac caagcgcgct
     1561 ttggcatcgc gtttggtgcg gtcggcgcgg ccatggcttg ttacgacgag gcgctgtcgt
     1621 acaccaaaga ccgcgtgcag tttgccggca agcccatcgc gtcgcaccag cttgtgcagc
     1681 tgaagcttgt cgacatgctg caagagatca ccaaagcgca gcttcttggt ttgcagcttg
     1741 ggcgactcaa agaagccggc aaggttcgtc cccagcaggt cagcctggcc aagcgtaaca
     1801 atgtgaaaat ggcgctcgag tgtgcccgca atgcccgcga tctcctcggg gccaatggca
     1861 tcaccgacga gtatcagagc ggtcgccaca tgtgcaacct tgagtcggtc ttcacttacg
     1921 aaggcaccga tcacatccac accctgatcg tcggcgagga catcaccggc tgttcggcct
     1981 tctagtcgcg cagacgctgg ctcagcctgg gcttttcaaa aacttgtcac acggacttct
     2041 caatctgcgt ggcaaggtcg cccagatccg caccgcatca ccctgctgta cgtcggccac
     2101 ggtgacagac gatgccgcta gtcagtacga aactgctggc gcaaacaata cgcgggggtc
     2161 cagcggctgt ttcacgagcg aaacaagcgt cggggggcgg ggttagcggg ccaggatgcc
     2221 ggggtttttg acttcgagct gggtcaggat ctgatcgacg cggcttgagt catcgacaca
     2281 cgccatcaca ccgtcgacgt atacgatcgt cttgccgggc tgtcccgcca cgctaccgac
     2341 tgcaatgtgg ggatgaagca gcgacacgtc acaccgcggc atgccgctca aaagcgcagg
     2401 gcttgcctcg ggcgcgccgc caccacaacc gatcgccagc accgtggcaa acggaagaag
     2461 tgcgcggatg cgccagttgc gagcagcctg cgtggatcgc gtcgtgtcgt gcatgtctct
     2521 ttccgccatc ctaacaccaa gcgacatcga tcgtctcggt ttcgcagcag tcggcctagc
     2581 gctcgtcttt gctggacggc gctgcggcgg cagtcatcgg cgctggggcc gccggggctg
     2641 cggatgtgcc gggcgccggg gccgctggcg ttgccggctt gggcttttgc accgtctgcg
     2701 gacgcggacc gatgatcatc agcatccgtc ggccttccat catcgctggt tgctcgacga
     2761 tgccgacgtc ctggcacgcc tcgaagacgc ggcgcagtac gttctgaccg acttcggggt
     2821 ggacgatctc gcggccacgg aactggatca ccagcttggc cttgttccct tcggcgagga
     2881 actcgcggat gtgcttcacc ttgaagtcga agtcgtgttc gtccgtcttg ggacggaact
     2941 tcatttcctt gacttcgatc acggtgcgct tcgcgcgttc ttcgcgttcc ttcttggccg
     3001 tctcatactt gaagcggcca tagtccatga tcttgcagac gggcggcaca gctttcggcg
     3061 agatctcgac caggtcgagt ccttcctcac gcgccatacg aagcgcatca ggagtttcca
     3121 gaatgccgat ctgttgaccg tcacttccga tcacgcggac gcgaggaatg cggatgcggt
     3181 ggttgactcg atactgctcg gaaggagagg ggcggctttc gggtctcagg gtcgtggctc
     3241 caatctgtgg gttcgcgaag agctgccaga aaatgccgtg tgcctcggtt ctcttccgac
     3301 gcgccgacgg gctagcagca aagagtagtc cactagtaac ccgaccagaa acttcacgca
     3361 agatcgaagt cccacaggta cgcgctgcgc cgggtactat ggcggtgtga atacttttcg
     3421 catcgtgacc ctgaacatct ggggcaacca tgcgccttgg agcgatcgcc gtcggttgat
     3481 tcgtgccgag ctcttgcgcc tggacgccga tgtggtggcg ctacaggaag tcctgcggcc
     3541 ccttggccct ggtacatcgc aagctgatga actggccgtt gacttggccc tgcctgcgcc
     3601 ctaccgggtt tgttttggca aggcctgcca gatcgacaag ccattccctg ccgagtttgg
     3661 caacgccgcg ctcattcgat cgcccctgcg tgagcagcgc gttgtgaagc tgccgacgcc
     3721 ggaaggcatc gagacccgat cggcgctgta catcctgctg tcggtcaagg tcggtctgtt
     3781 gccggtcgtc atcacccacc tgtcgtggga gccggagctg tccgaaaccc gcgccgccca
     3841 gcttcgtcac atccagcagt ttctgaccgg cgaaatggcc gcgctgccga cgcgcgttcc
     3901 gccgcatgtt ccgatcttgc cgccgcttct gcttggggat ctaaacgccc caccgggcag
     3961 tgccgaaatc caagtcctga ccgcaccggc accggggacg ctggcctttg tcgacggctt
     4021 tgcgcaggtt ggcgcgggcc cagcagagac cttttc
//
LOCUS       DAORGN010000309         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12228_length_4035_cov_6.020854, whole genome shotgun sequence.
ACCESSION   DAORGN010000309 DAORGN010000000
VERSION     DAORGN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12228_length_4035_cov_6.020854"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKL17_20855"
     CDS             <1..209
                     /locus_tag="PKL17_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47245.1"
                     /translation="VDAGEDSRFIADLCSASAVYSSGTLGYRIFIDEDSQYEIRKRRI
                     RFTSSELELRKIEENEWVEWFPLC"
     gene            352..756
                     /locus_tag="PKL17_20860"
     CDS             352..756
                     /locus_tag="PKL17_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47246.1"
                     /translation="MQIESTIQKVMRDIPECVATGYVDMSTGMLLAVKTIDSHPREVL
                     DLVAAATADLFEGSTVTAIEKLFRRARGVKDDGHHYFQEMVVFSDNLLHVFLRGRKNT
                     EHVVCFVCRKSANIGMVLTKSRLAMPTIESAV"
     gene            862..1536
                     /locus_tag="PKL17_20865"
     CDS             862..1536
                     /locus_tag="PKL17_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47247.1"
                     /translation="MAKAAFSQPKTPMQMVDALNNRVAASARSYKMATVRYLLRPPLS
                     LRAKYAAFLLDFAQQSDLISDVLGRAGERVADPAVAELLRKHQREEKARVQLVLSDLR
                     RLGYSDELPDRPMTSGDAALRAYCSSVSGERPYAMLGVMLALFGVAAEVSSTVVRLLS
                     VGSVPREAMRWLLLRQSEDPQELRTLLEKTAELIRDPVEQSQLLEAVEVTGELLALGA
                     AARPAA"
     gene            complement(1520..2899)
                     /locus_tag="PKL17_20870"
     CDS             complement(1520..2899)
                     /locus_tag="PKL17_20870"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNK47248.1"
                     /translation="MSRPGYSQTLEFLAQLSPLGIQPGLSRIADVLRRLEFPEQRYPS
                     VHIAGTNGKGSTSAYAASIANVAARRAAEHLGRPLAIGLYTSPHLHRLTERICFGANG
                     QLNECPKQTFADAVAQVRAVAETEPTVPLTFFEFVTAAAFLLFAQQKVDLAIIETGLG
                     GRLDATKLCAAQVTVVTSIGLDHMDWLGPSIAHIAYEKAGIFRPGVPTLCATYPEEAR
                     QVLRRVAKETAAPLFMHPYHGEPDAAPLPLLSDELRAVLPLPGAHQADNASLAVAALS
                     HVDGPLRPFLHDPDVIREGLAKTRWPGRLERIVPTAKAYQPFANREIIVDAAHNPEGA
                     QVLSAWLEREPPASLTVICGVVVGKLATEMAAPLAHASRIILCRPPTPRGLPAAELAQ
                     VPGFSRGEIEDDWHAALDLALAKLPQNGRMLVYGSIFLIAAVRAALLDEPTDELLVQD
                     PGRRATPPV"
     gene            complement(2896..3795)
                     /gene="accD"
                     /locus_tag="PKL17_20875"
     CDS             complement(2896..3795)
                     /gene="accD"
                     /locus_tag="PKL17_20875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNK47249.1"
                     /translation="MAWWSRKNKATELASEKRSVPTGLFIKCDGCSATVDSEKIREAL
                     RCCPQCGFHFTMPTEERIELLLDEDSAEEQDLEIAPQDPLGFRDSKSYADRLVALKKH
                     TGQPDAFRSFLAKLDGVPVSFGMFQFEFMGGSMGSVVGEKITRVFERAAQRRIPAIVL
                     SASGGARMQEGILSLLQMAKTSAALQRLRDLGVPYVSVLLHPTTGGVAASFAMLGDVI
                     LAEPGAMIGFAGPRVIEQTIRQKLPEGFQRAEFLLKHGIIDSIVPRAELKPRLGQLLR
                     LLRAEPKPQLTPPPDSTKSPAQS"
     gene            complement(3822..>4035)
                     /locus_tag="PKL17_20880"
     CDS             complement(3822..>4035)
                     /locus_tag="PKL17_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47250.1"
                     /translation="KQKGTAVLGPMSYVPAVAVLGGQLVVLSGGKAGSGQLKPGLYLG
                     TATGTALPTEPFDFGLPPSSVALVSR"
BASE COUNT          812 a         1163 c         1263 g          797 t
ORIGIN      
        1 aagttgacgc aggggaagat tctcgattca tcgctgacct ttgttccgca tcggctgtat
       61 acagtagcgg cacactaggt tatcgtattt ttatcgacga agattcgcaa tatgagatac
      121 gtaaaaggcg cattcgattc acttcgtctg aactggaact tcgaaaaata gaagaaaatg
      181 aatgggtaga atggttccca ttatgttgat tttttgattg atgctggtga aaatggaaat
      241 agcctaatga tttcgtgtat ttgagtattc cgattgtaac ttttgtggtt tttgctaact
      301 ctttggcctg cgcaaacaga caaatgtgag cttgttccct ggagtcaaag aatgcagatc
      361 gagagcacaa tacagaaagt gatgcgcgat attccggagt gcgtggccac aggttacgtt
      421 gacatgtcga ccggaatgct gcttgcggta aagacgatcg attcccaccc gcgtgaagtc
      481 ctcgatctgg tggcggcagc cacggcagat ctattcgaag gcagcacggt gacggcgatc
      541 gaaaagctgt ttcgacgggc gcgcggcgtc aaagacgacg gacaccacta ctttcaggag
      601 atggtggtgt tttccgacaa cctgctgcac gtttttctgc gcggacgcaa gaacaccgaa
      661 catgtggtgt gcttcgtctg ccgcaagagc gcgaacatcg gaatggtgct caccaagtcc
      721 cgtcttgcca tgcccaccat cgagagcgcc gtctagaagc gtgcccaagt aatcctgcgt
      781 ggcaggttgg cagcgcctgt gcggtcttct tcggtggcgg acccaagcta atggagttag
      841 cctaggaagt gtagaaactc catggccaaa gcggctttta gccagcccaa gacgccgatg
      901 cagatggtcg acgctttaaa caaccgagtg gcagcttccg cacgtagcta caagatggcg
      961 accgtgcgct acctgctccg tcctcctctt tcattgcgtg ccaagtacgc tgcgtttctc
     1021 ctcgatttcg cccagcagag cgacctgatt tcggatgtgc tcgggcgggc aggggagcgc
     1081 gtcgctgatc cggcggtggc agagcttctg cgcaagcatc agcgcgaaga aaaagcgcgc
     1141 gtccagttgg tgttgtcgga tctgcggcga ctcggctact cagacgagct accggacagg
     1201 ccgatgacat ctggcgacgc ggcgctgcgg gcgtactgtt ccagcgtcag cggtgagagg
     1261 ccgtatgcga tgctgggggt gatgctggcg ctgtttggcg tcgcggcaga agtcagctcg
     1321 acggtggtgc ggcttctgtc ggtcggtagc gtgccgcggg aagcgatgcg ctggctgctt
     1381 ttgcggcagt cggaagatcc gcaagagctg cgcacgttgc tggaaaaaac ggcggagctt
     1441 atccgtgatc ccgtcgagca gtcgcagctg ttggaagccg tggaagtcac cggcgagctg
     1501 ctggcgctcg gggcggcggc tagaccggcg gcgtagcgcg acgaccgggg tcttggacca
     1561 gcagctcgtc tgtcggttca tcgagcagcg ccgcacgcac cgccgcgatc aaaaaaatcg
     1621 acccgtagac aagcatccgg ccattttggg gcagcttggc cagcgcaagg tcgagcgctg
     1681 cgtgccagtc gtcctcgatt tcgccgcgcg aaaaaccggg cacctgcgca agctcggcgg
     1741 cgggaagacc gcgtggcgtc ggtgggcgac acaagatgat ccgcgaggca tgcgcaagcg
     1801 gggcagccat ttctgtcgcc agtttgccga cgacgacccc acaaatcaca gtaagcgacg
     1861 cgggcggctc tctttctaac cacgctgaaa gcacctgcgc accttcggga ttgtgcgctg
     1921 cgtcgacgat gatttcgcga ttggcaaacg gctgataggc cttggccgtc ggaacaatcc
     1981 gttccagtcg gcctggccag cgtgtcttgg caagtccttc gcggatgacg tcgggatcgt
     2041 gcaaaaacgg tcgcagcggc ccgtctacgt gcgaaagcgc cgccaccgca agcgaggcat
     2101 tgtctgcttg gtgggcaccg ggcagcggca acaccgcgcg aagttcgtcg gacagcagcg
     2161 gcagcggcgc ggcatcgggt tcgccgtggt acgggtgcat aaacagcggg gccgccgttt
     2221 ccttggccac ccgccgcagc acttgtcgcg cttcttctgg ataggtcgca cacagcgtcg
     2281 gcacacccgg tcggaaaatc ccggcttttt catacgcaat gtgcgcaatc gacggcccca
     2341 gccagtccat gtgatcaagt ccgatgctcg tcaccaccgt gacctgcgcg gcacagagct
     2401 tggtcgcatc cagccgtccc ccaagccccg tttcgatgat cgccaagtcg accttttgct
     2461 gggcgaacag cagaaaggcc gccgcggtca caaactcaaa aaatgtcagt ggcacggtcg
     2521 gttccgtctc tgcgacggcg cggacttggg ccactgcatc ggcgaatgtt tgtttggggc
     2581 actcattgag ctggccgttt gcgccaaaac aaatccgctc ggtcagccgg tgcaggtgcg
     2641 gcgacgtgta aagaccgatg gcgagcgggc gtcctaggtg ctcggctgcg cgacgggccg
     2701 cgacgttggc aatcgacgcg gcataggcgc tggtggaccc tttgccgttg gtcccggcga
     2761 tgtgtacgga cggatagcgc tgctcgggaa attccaagcg gcgcaatacg tccgcgatgc
     2821 gggaaagccc tggctgaatg cccagcggtg acagctgggc gagaaactcc agcgtctgcg
     2881 aatagccagg ccggctcacg actgcgccgg actcttcgtc gaatcgggcg gcggagtcag
     2941 ttggggtttg ggctcggcgc gcagaagccg caagagctga ccaaggcgcg gcttgagctc
     3001 ggcgcggggc acgatgctgt caatgatgcc gtgcttgagc aaaaattccg cgcgctggaa
     3061 gccttcgggc agcttctgcc gaatcgtctg ttcgatgacg cgcggtccgg caaagccgat
     3121 catcgcgccc ggttcggcca agattacgtc gcccagcatt gcaaacgacg ccgcgacacc
     3181 gccggtcgtt ggatggagca gcaccgagac atacggcacc cccagatcgc gcagcctttg
     3241 cagcgccgcc gacgtcttgg ccatctgcaa aagcgacaga atgccttcct gcatgcgcgc
     3301 accgcccgac gctgacagca cgatcgccgg aatccgacgc tgggcggctc gctcgaagac
     3361 gcgggtgatt ttttcaccga cgaccgatcc catcgaaccg cccataaact cgaactgaaa
     3421 catcccaaat gaaaccggga ctccgtcgag cttggcaagg aagctgcgga atgcgtcggg
     3481 ctgtccggtg tgttttttca gcgccaccaa gcgatcggcg tagcttttgc tgtcgcgaaa
     3541 gccgagcgga tcttgcggtg caatctccag gtcttgctcc tccgccgagt cttcgtcgag
     3601 gagcagctcg atccgttcct ccgtcggcat tgtgaagtga aaaccgcact gtggacagca
     3661 gcgcagcgct tcgcggattt tttcggaatc caccgtcgct gagcagccat cacacttgat
     3721 aaagagaccc gtcggaaccg agcgcttttc ggaagcaagc tcggtggcct tgttttttcg
     3781 cgaccaccac gccatgtgca gcaagcaggg tataggccaa actatcgtga gacaagagcc
     3841 acggagcttg gtggcagacc gaaatcgaaa ggctcggtcg gaagcgcagt tcccgtcgct
     3901 gtcccaagat acagaccggg ttttagctgt ccgctgccgg ccttgccgcc cgatagtacg
     3961 accagttggc caccgaggac ggccaccgcg gggacgtagc tcatcggacc gagcacagcg
     4021 gtgcctttct gtttg
//
LOCUS       DAORGN010000310         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12246_length_4031_cov_7.162726, whole genome shotgun sequence.
ACCESSION   DAORGN010000310 DAORGN010000000
VERSION     DAORGN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12246_length_4031_cov_7.162726"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKL17_20885"
     CDS             <1..718
                     /locus_tag="PKL17_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47251.1"
                     /translation="TLDMALFAIMQAMVKTKKMASLLRTAAPAAACAVLLAAQPADAK
                     VLEGYVDVYLGGMYGTVPKLTSIVTQQSEPNGAGISKGADFFDYNSGGLIGLRGGIEV
                     LHTDFYVQFDQFLNLKGASGSTIQGMIGWDFELGSGSWRGTLGAYGGLIFGLPYRTEF
                     PIDRKQIAWFGVGAEGQAGVEYYLNRFFVLNFTGTFGYHYMFAGADPIVIDMAGNTEQ
                     TRTHGFHLLGKLGLRFQLGI"
     gene            759..1658
                     /locus_tag="PKL17_20890"
     CDS             759..1658
                     /locus_tag="PKL17_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNK47252.1"
                     /translation="MSHAAKPVVITCALTGVLTDPGQHRVPVTAAEMAAACAEARDAG
                     ATVVHCHYRQQAPGMGRFPTWDPEVVAEIDAAIRARVPDIIVNMSTGVIGPDVSAPLA
                     CLSRIRPEMAALNAGSLNYLKARSDGRWAWPPMLFDNPVDKIEKFLAAMREVGTVPEC
                     ECFDTGIVRSIGLFKQVGLLTGPIHVSLVMGVASGMPCKPEWLPLIVSELPEGAHFQT
                     IGIGQREVWPVHRRAAELGGHLRTGLEDTFYLPDGSKTDSNGKLIEALVQIAREVGRP
                     IATAAEARAICGVLPMPHGPQGA"
     gene            1655..3118
                     /locus_tag="PKL17_20895"
     CDS             1655..3118
                     /locus_tag="PKL17_20895"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNK47253.1"
                     /translation="MSRLTKVHPARRMVVWTADAGHGRHLAESLRKDGYSVRAVTSQA
                     AMESALSDGADLLVAELADCDLALVAFLAARCPMLFVAIPQPQPDSVTELVTVGAYDV
                     LASPLRAVDVRLALHKAQAREASLGDRTAGRFLPSPLPETKSGVSLPGVIAESKVMQS
                     LLAQVARVAVHPTSVLLLGESGTGKEVLAKAVHQLSPRRDKPFVAINCGALPAQLLES
                     LLFGHVQGAFTDAIRDQQGVFQRADGGTLFLDEIGELPQTLQVKLLRALQEQKILPLG
                     ARDEISVDVRVIAATLRNLSREVDAGRFRADLYYRLSVVELVVPPLRERTADILPLAQ
                     HFLRKAAQRLARPVVSLSDAAQKVLLRFPFPGNVRELENLIERAVVLCERTQLDVTDL
                     PYEVVQPRPAAIGPDHADPSPRDEAPTGDLSIKAATARLERELIARALHKTAGNRAAA
                     ARMLELSHRALLYKLREYGLDEKPASKPTASDRSSRG"
     gene            3158..>4031
                     /locus_tag="PKL17_20900"
     CDS             3158..>4031
                     /locus_tag="PKL17_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNK47254.1"
                     /translation="MSATASGRFYLTTPIYYVSDIPHIGHAYTTIVGDAIARYERLRR
                     GGENVFYLTGTDEHGEKLERAAQAKAMSPQSFVDLIAEEYKKTWQILEIQNDDFIRTT
                     DPRHEKVVQELWRKLVDVGDIYLDRYEGLYCVGCENFLLEKDLLPGNLCPDHKRPVEK
                     RSEESYFFRLSKYQQKLLDLYAQVPDFISPPRRMNEVRTFVESGLRDLSVSRSSFKWG
                     VEVPGDPRHVIYVWIDALTNYYSALQDSPHKAAFWGTHDAPLAIHLVGKEIVRFHAVY
                     WPAMLMAAGLPLPRK"
BASE COUNT          766 a         1234 c         1231 g          800 t
ORIGIN      
        1 gacgctagac atggcgctat ttgctatcat gcaagccatg gtgaaaacga aaaaaatggc
       61 aagcctgctt cgcacggcgg cgccggcggc ggcttgtgct gtgctcctcg ccgcgcagcc
      121 agccgatgcc aaagtcctcg aaggctacgt cgatgtgtac ctcggtggaa tgtatggcac
      181 ggtaccgaag ctcacgtcga tcgtgaccca gcagtcggag ccaaacggcg ctggcatcag
      241 caaaggcgct gacttcttcg actacaactc gggcggattg atcggtctgc gcggtggtat
      301 cgaagttttg cacaccgact tctacgtcca gtttgaccag ttcctcaacc tcaagggcgc
      361 gtcgggatcg acgattcagg gcatgatcgg ctgggacttc gagctgggct cgggttcgtg
      421 gcgcggcacg ctgggcgcat acggcggact gatttttggc ctgccatacc gcaccgaatt
      481 tcccatcgac cgcaagcaga tcgcgtggtt cggcgtcggt gcggaaggtc aagccggcgt
      541 cgaatattac ctaaaccgct tcttcgtttt gaacttcacc ggcacgtttg gctaccacta
      601 catgttcgct ggcgccgatc cgatcgtcat cgacatggca ggcaacaccg aacagacccg
      661 cacccacggc ttccacctgc ttggcaagct cggcctccgc ttccagctcg gcatctaatt
      721 ggccattttc cagaccgggg ctttgatatc gtcccggtat gagtcacgca gcaaagcccg
      781 ttgtcatcac ctgtgcactg accggcgttc tcaccgaccc gggtcagcat cgcgtccctg
      841 tcaccgcggc ggaaatggct gccgcttgtg ccgaggcgcg tgatgccggt gcgacggtgg
      901 ttcattgtca ctaccgacag caagcccccg gcatgggccg attcccgacg tgggaccccg
      961 aagtcgtcgc cgaaatcgac gccgcgattc gcgcccgcgt gcccgacatc atcgtcaaca
     1021 tgtcgaccgg cgtgattgga cccgatgtca gcgcgccact cgcctgtctg tcacgcatcc
     1081 gtcccgagat ggccgcgctc aacgccggat cgctcaatta tttgaaagcg cgcagtgacg
     1141 gtcgctgggc ctggccgccg atgctgttcg acaaccccgt cgacaagatc gaaaaattcc
     1201 tggctgcaat gcgcgaagtc ggcaccgtcc ccgagtgtga gtgcttcgac accggcatcg
     1261 tccgctcgat cggtcttttc aaacaggtcg gtctgctgac gggcccgatc catgtctcgc
     1321 tggtgatggg cgtcgcaagt ggaatgccgt gcaaacccga atggctgccg ctcatcgtct
     1381 ccgagctgcc cgagggcgcg cacttccaaa cgattggcat tggtcagcgt gaagtctggc
     1441 cggtgcaccg acgcgctgcc gagctgggtg gccacctgcg taccggactc gaagatacgt
     1501 tttatcttcc cgacggcagc aagaccgatt caaacggcaa gctgatcgaa gcgctggtcc
     1561 agatagcccg cgaagtcggt cgtccgattg ccaccgccgc ggaggcccgc gcgatctgcg
     1621 gcgttttgcc gatgccgcac ggaccacagg gcgcgtgagc cgattgacca aagtacatcc
     1681 agcccgtcgg atggtcgtct ggactgccga tgctggacac ggacgacacc tggctgaatc
     1741 gctgcgaaaa gacggttatt cggtccgcgc agtgacctcg caagccgcga tggaatcggc
     1801 gctttccgat ggcgccgact tgctggtcgc agagcttgcc gattgcgatt tggcgcttgt
     1861 cgctttcctt gccgctcgct gtccgatgct gtttgtcgcg attccgcagc cgcagccaga
     1921 ttcggtgact gaacttgtga ccgtcggtgc atacgatgtg ctggcgtcgc ctttgcgcgc
     1981 ggtcgatgtc cggctggccc tgcacaaagc acaggcgcgc gaagcgtcgc ttggggatcg
     2041 cacggcgggt cgatttttgc caagcccgct tcccgaaacc aaaagcggcg tctcactgcc
     2101 cggcgtcatt gccgaatcaa aggtgatgca gagtctcttg gcgcaagtgg cgcgcgtcgc
     2161 ggtgcatccg acatccgtgc tgctgctcgg ggaatcggga accggcaaag aagtgctggc
     2221 caaagcagtg catcagcttt cgccgcgacg cgacaagccg tttgtggcga taaactgcgg
     2281 cgcgctgccg gctcagctat tggaaagtct gctgtttggt cacgtccaag gcgcgttcac
     2341 cgacgccatt cgtgatcagc agggtgtgtt tcagcgcgcc gacgggggaa cgctgtttct
     2401 cgatgaaatt ggcgagctgc cgcagacgct gcaagtgaag cttttgcgcg cactgcaaga
     2461 acagaagatt ctgccgctcg gcgcacggga cgaaatttcg gtcgatgtgc gggtgattgc
     2521 ggcgacgctg cgcaacctgt cgcgcgaagt cgatgcggga cgctttcgcg ccgatttgta
     2581 ctatcgctta agcgtcgtcg agctggtcgt gccgccgctg cgcgagcgaa ctgccgacat
     2641 tttgccgctc gcccagcact ttctgcgcaa agcggcccag cggctggcac gtccggtggt
     2701 gagtctttcc gatgcagcac aaaaagtcct tttgcggttt ccattccccg gcaacgtgcg
     2761 cgaactggaa aacctgatcg agcgcgcggt ggtgctgtgc gaacggacac agctcgacgt
     2821 gaccgacttg ccatatgaag tggtgcagcc acggcctgcg gcgatcggtc cggaccacgc
     2881 cgatccaagc ccacgcgacg aagcgccgac cggcgatttg tcgatcaaag cagccacggc
     2941 acgactggag cgcgagctga ttgcgcgtgc tctgcacaaa acagcaggca atcgagcggc
     3001 ggcggcgcgg atgttggagc tgtcgcaccg ggccctgctt tacaagctgc gcgaatacgg
     3061 gctcgacgaa aaaccggcca gcaagccgac cgcgagcgac cgcagttctc ggggctaggt
     3121 tgacggcgcg ggtttgcagc ctataccttt gcggcccatg tcagccacag ccagcggtcg
     3181 tttttatctg acgacaccga tttattacgt atcggacatt ccgcacatcg gccatgccta
     3241 cacgacgatt gtcggcgacg cgattgcacg ctacgagcgg ctgcgtcggg gcggcgaaaa
     3301 cgtcttttac ctgaccggca ccgacgaaca cggcgaaaag ctagagcggg cggcccaggc
     3361 caaagcgatg tcgccgcagt cgtttgtcga tctcatcgcc gaggaataca aaaagacctg
     3421 gcagatcctc gagatccaaa acgacgactt cattcgcacc accgatcctc gtcatgaaaa
     3481 agtcgtgcag gaactgtggc gcaagctcgt cgacgtgggt gacatctatc tcgatcgcta
     3541 cgaaggtctt tactgcgtcg gctgtgaaaa cttcctgctc gaaaaagacc tgctgccggg
     3601 caatctgtgt cccgatcaca agcgtccggt cgaaaaacgc agcgaagaga gctacttttt
     3661 ccgtctgtcg aagtaccagc agaagctgct cgatctgtat gcgcaggtgc cggactttat
     3721 ttcgccgccg cgccgcatga acgaagtgcg gacgtttgtc gaatcgggtc tgcgcgatct
     3781 gtcggtgtcg cgctcgtcgt tcaagtgggg cgtcgaagtt ccgggcgatc cgcgccacgt
     3841 catctacgtc tggatcgacg cgctgaccaa ctactattcg gcgctgcaag acagtccgca
     3901 caaagcggcg ttttggggca cccacgacgc gccgctcgcg atacacctcg tcggcaaaga
     3961 gatcgttcgt tttcatgcgg tgtactggcc ggcgatgctg atggcggcgg gactgccttt
     4021 gccgcgcaag a
//
LOCUS       DAORGN010000311         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12252_length_4028_cov_6.578908, whole genome shotgun sequence.
ACCESSION   DAORGN010000311 DAORGN010000000
VERSION     DAORGN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12252_length_4028_cov_6.578908"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKL17_20905"
     CDS             complement(<1..863)
                     /locus_tag="PKL17_20905"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNK47255.1"
                     /translation="MEFLVFLCFLLSGGSGLLFEVLWTRELQLVFGSTTLAMSTVLSV
                     FMGGLALGSLLGGRLAPKVRRPLVAYALVEAGVGLYALLLPLFLARYPALNAALWRTV
                     GDHFVFLSALRFLATALVLIIPTTLMGATLPLLSQYVAQKHAGIVGTSVQIGTLFAMN
                     TTGAVVGTFLSGFVLLPRFGVSQTNLIGACTNLSLAALILFGSALYRAKPLRAAEPEV
                     PDLTASDEESSEDEAQADAGVEPSPLYQRRLAVLSYAISGATAMVYQVLWTRALAIII
                     GSSVYSFTLILL"
     gene            complement(906..2156)
                     /locus_tag="PKL17_20910"
     CDS             complement(906..2156)
                     /locus_tag="PKL17_20910"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK47256.1"
                     /translation="MLVKAGQLLDVRTGVLLQKQALLIENERVKEVGPQAQVMAHAPS
                     GAQLVDLSGYTVLPGLIDAHTHLTYRVDVASYEWLGVSAPREALLGARHARATLLAGF
                     TTVRNLGARGYADVALREAIKDGDVIGPRMQVSGPAIGSTGGHMDSNLLPYAFRYSEE
                     GVADGREGVIRKVREVVKYGSDVIKVAVTGGFLSVGTNPNVGQYSDEELSSLVAEAKR
                     LGKPVAAHAHGTVGIKQAVRAGVDSIEHATELDDECIQLMKSRGTFLVPTVFLWSHFP
                     ENARPPQVPQEKVEKAREMAPIAKKNLSKAVKAGVKIAFGTDSSVYPHGMNGHEFGAL
                     VELGMSPLGAIQAATIHAAKLLGWDTQIGQLQPGFYADLVAVPSNPLANVRVLENIPF
                     VMKGGEVFKDERSPACGPQSTPKP"
     gene            2425..3231
                     /locus_tag="PKL17_20915"
     CDS             2425..3231
                     /locus_tag="PKL17_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47257.1"
                     /translation="MSAKHDLRPWYASLPLTSLILTAPFVFVALCGNKWSPTDSTFTT
                     LAKPKEPPSVQIAESHHETLLTATSWLAYPPHHFARCDVQPGKHPRFAVCFDRHGTPI
                     LLRANVEQLVQIGLQVSAPVRGRWLLDGHRYLLGETLIETKPQLQIAPLPRPLADERL
                     ATPEQPRLVSLSRDEEFALWEDRRPRPDGAVQLLVSRVSDGRIESARTVNDPTQVARL
                     RDPNVREELTQQIVFWTDQGRRQHVELPGDNQSEQEPREAAIEQNEVSAE"
     gene            complement(3379..3810)
                     /gene="rplQ"
                     /locus_tag="PKL17_20920"
     CDS             complement(3379..3810)
                     /gene="rplQ"
                     /locus_tag="PKL17_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNK47258.1"
                     /translation="MRHQKSGRTFGRKADHRKALWSNMVSSLIQAERIQTTDVKAKEL
                     RRFAEPAIAWAVSVGSLLSKDEAKRTAEEKTRIVHAMRMAQRIVKNREALHKLFDELG
                     PRYASRHGGYLRIIKKGFRHGDAAPVSIVELVDRPEAGAQA"
     gene            complement(3831..>4028)
                     /locus_tag="PKL17_20925"
     CDS             complement(3831..>4028)
                     /locus_tag="PKL17_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNK47259.1"
                     /translation="SANCLQNANIRYIGELVQRSEAEMLKTKNFGRKSLKEIKEILAE
                     MNLSLGMKLEGWEAPSSRNKL"
BASE COUNT          804 a         1333 c         1120 g          771 t
ORIGIN      
        1 agcaggatca gcgtgaacga atacaccgac gatccaatga tgatcgccag cgccctcgtc
       61 cacagcacct gatagaccat cgccgtcgcg cccgagatcg cataggacag caccgccagc
      121 cgtcgctggt acagcggact cggttcgacg ccggcatcgg cttgcgcctc gtcttcactg
      181 gattcttcgt cgctggccgt cagatcgggc acttccggtt ccgccgcgcg caaaggcttt
      241 gcccgataca gcgcactgcc aaacaggatc aaggccgcca ggctcaggtt tgtgcaggcg
      301 ccgatcaagt tggtctgcga cacgccaaac cgcggcagca gcacaaagcc cgacaaaaac
      361 gtgccgacca ccgcccccgt cgtgttcatc gcgaacagcg ttccgatctg caccgatgtg
      421 cccacgattc cggcgtgttt ttgcgcaacg tactgcgaca gaagcggcag cgtcgccccc
      481 atcagcgtcg tcgggatgat cagcacaagt gcggtggcca aaaagcgcag cgccgacaaa
      541 aagacaaagt gatcgccgac ggtccgccac agcgccgcat tgagtgccgg atagcgagcc
      601 aaaaacagtg gcagaagcag cgcatacagc ccaacgccgg cttcgacaag cgcatatgcg
      661 acgagcggac gtcgaacctt gggcgcgagc ctgccgccca gcagactgcc aagcgccagc
      721 ccacccatga acaccgacag caccgtcgac atcgccagcg tcgtcgatcc gaacacgagc
      781 tgcagctctc gcgtccacag cacttcaaac agaagccccg atccaccgga gagcaggaag
      841 caaagaaaga ccagaaattc catgcgggcg cagcgtaacc ccctcttttg ggcgctgcga
      901 aggaattagg gtttgggcgt gctctgcggg ccacaagccg ggctgcgttc gtctttgaac
      961 acttcgccgc ctttcatcac aaacgggatg ttttcgagca cccgtacatt cgcgagcgga
     1021 ttggacggca ccgccaccag atcggcgtaa aagcccggct gaagctgccc gatctgcgtg
     1081 tcccacccga gcagcttggc cgcatggatt gtcgcggcct ggatcgcccc aagcggcgac
     1141 atcccgagtt cgaccagagc gccaaactcg tggccgttca tgccgtgcgg atacaccgaa
     1201 gagtcggtgc caaacgcaat ctttacgccc gcttttaccg cttttgagag gtttttcttc
     1261 gctatcggcg ccatttcacg cgctttttcc actttttctt gcggaacctg cggcggacgg
     1321 gcgttttcgg gaaaatgcga ccacagaaac accgtcggaa ccaaaaacgt gccgcgcgac
     1381 ttcatcagct gaatgcactc gtcgtccagc tccgtcgcgt gttctatcga atcgacgcca
     1441 gcccgcaccg cctgtttgat cccgactgtg ccgtgcgcat gggctgccac cggcttgccc
     1501 agccgcttgg cttccgccac cagcgagctt agctcttcat cgctgtactg gccgacgttg
     1561 ggatttgtgc cgaccgatag aaaaccgccg gtcaccgcca ctttgatcac gtccgagccg
     1621 tacttgacca cttcgcggac tttgcgaatc acaccttcgc gtccgtcggc aacgccttcc
     1681 tcgctgtagc gaaacgcata cggcagaagg ttcgaatcca tgtgtccgcc cgtcgagcca
     1741 atcgccggac cagacacctg catgcgcggc ccgatgacgt cgccatcctt gatcgcttcg
     1801 cgcagcgcca catcggcata accgcgcgcg cccaggttcc gcaccgtagt aaaacccgcc
     1861 agcagcgtcg cccgcgcgtg cctcgccccc agcagcgctt cgcgtggcgc cgacacccca
     1921 agccactcgt aacttgcgac atcgactcga tacgtcaggt gtgtatgtgc gtcgatcagc
     1981 ccaggcagca cggtgtagcc actcagatcg acgagctgcg ctcctgacgg cgcgtgcgcc
     2041 atgacttggg cttgtggacc gacctctttg acccgctcat tttcgatgag cagcgcctgc
     2101 ttttgtaaca gcacccctgt ccgaacatcg agaagctggc ccgccttgac cagcactgga
     2161 cctcttgctc ctgcttccgc cattcccccg ctgagcaggc agagcaaccc ccaagtacat
     2221 cccacaactc gtgcggcccc cattgaactg tctcctctgc cgattgatcg ggctattttc
     2281 ccgcggctca acgattcctt cgaccgtttt ctgtatttat ggctacatat acagagggtc
     2341 gaccatgtgg ttttgcgtcc cccgtggctg ctttttgcgg caaggagtac gtcgcggtta
     2401 agcttgcagt gaggagagga ctcgatgtcg gccaagcacg atctgcggcc atggtatgcc
     2461 tcgctcccgc tcaccagctt gatcctgacg gcaccatttg tgtttgtggc gctgtgcggc
     2521 aacaagtgga gccccaccga cagcacgttc acgacgctgg ccaaaccgaa agagccgccg
     2581 tctgtccaga tcgcagagag ccatcacgaa accctgctta cagcgacgag ctggctggcg
     2641 tacccgccgc accactttgc gcgctgtgat gtgcagcctg gcaaacatcc ccgctttgcc
     2701 gtctgcttcg accggcacgg cacgccgatc cttttgcgcg ccaacgtcga acagctggtc
     2761 cagatcggtc ttcaggtcag tgcgccggtc cgcggacgct ggctgctcga tggccatcgc
     2821 tatcttctgg gcgagacctt gatcgaaacc aagccgcagc ttcagatcgc gccgctgcca
     2881 agaccgctgg ccgatgaacg cctggcgaca ccggagcagc cacggcttgt ctccctgtcg
     2941 cgggatgaag agttcgcgct gtgggaagac cgccggccaa ggcccgatgg cgcagtgcag
     3001 ctgcttgtga gtcgcgtgtc agatggtcgt atcgagtcgg cgcgtacagt caacgacccg
     3061 acgcaagtgg cacggcttcg cgatcccaac gtgcgcgagg agctcaccca gcagatcgtc
     3121 ttctggaccg atcagggacg ccgccagcat gtcgagctgc ccggcgacaa ccaaagcgaa
     3181 caggaaccgc gcgaagcagc gatcgagcaa aacgaagtca gcgcggaatg aacgcgacgc
     3241 aagcaatccg ccgcggcgcg ggtggatggg tcatcacgaa acaacgcgac gaagcgaatc
     3301 agtcgccaaa aatgaaaaag cgccgcaggt cataccgacc ggcagcgctt tgcaagagcg
     3361 agggtttgtt cgcgcagact aggcttgggc gcccgcttcc ggacgatcaa ccagctccac
     3421 gatcgacacc ggcgcggcgt caccgtgacg gaaacccttt ttgatgatgc gaaggtagcc
     3481 accatggcgg ctcgcgtagc gcggtcccag ctcatcaaag agcttgtgga gcgcttcgcg
     3541 gtttttcacg atccgctgag ccatccgcat cgcatgcacg atacgggtct tttcttcggc
     3601 ggtgcgcttc gcttcgtctt tgctcaaaag gctgccgacc gaaacggccc acgcaatcgc
     3661 tggctctgca aaccgtcgca gttccttggc cttcacatcg gtggtctgga ttcgctcggc
     3721 ctggatgagc gacgaaacca tgttcgacca caacgccttc cggtgatcgg ccttgcggcc
     3781 aaacgttctg ccagattttt ggtgacgcat tgtgcttgtc tctgcgcttc ttaaagcttg
     3841 ttacggctcg acggagcttc ccagccctcg agcttcattc ccagcgacag attcatctca
     3901 gccaggattt ctttgatttc tttgagcgac ttccgaccaa agttcttggt cttcagcatc
     3961 tccgcttcac tgcgctgcac cagctcgcca atgtaacgaa tgttggcgtt ctgcagacag
     4021 ttcgccga
//
LOCUS       DAORGN010000312         3974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12545_length_3974_cov_5.556520, whole genome shotgun sequence.
ACCESSION   DAORGN010000312 DAORGN010000000
VERSION     DAORGN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_3974_cov_5.556520"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="PKL17_20930"
     CDS             complement(<1..1678)
                     /locus_tag="PKL17_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47260.1"
                     /translation="MLPSSPAEITPPPIVTSPFARLQSYLSQVRRRLLRREKLRLLLL
                     WLQATGVCGAAVLLLAAQTGPHWAWLPICGVYALLALLALAVLQVRARSERTSDEAMA
                     RFVGARLPDLRSELLSTIELQKELTNGPERALVEELAQRTLAALGTVQPDSLVDRWPV
                     WQAGLSALMLFGFGLFATLAGALPLAKGLHTLGTVSPPEPVSIATEPLLGDIRLLLVY
                     PKYTGLHQRTIPGSTGDILALPGTQVRIEAWALVPVERAQITVIADGQESSQPVQVGK
                     PKAGQDAPLPLLSASLVVQKAGSYYFVIKNGHKALLREGQSHRIAIESDHPPRIDLFA
                     PAAELEVTSTRRIELAYSAEDDFGLGDIDLVYRIGSGPERRRRLRSAPTGDSKPDDKP
                     TQKNGINTPTVPRNLAAKIEWDLSELELQPGVRVTYFAEARDLNNVTGPGVGRSREYA
                     LFIQSPRQRQEALLQSQSQLREQAVQLLADRIDLLRGLTREPKDQTDLSTEAADRVQA
                     AHRKSETFLLLIGRVQQDSARGEKPAKDLLAALQEIADRLGKLNQEEAALL"
     gene            1817..3277
                     /locus_tag="PKL17_20935"
     CDS             1817..3277
                     /locus_tag="PKL17_20935"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNK47261.1"
                     /translation="MGRELPQGTAVGAYSIERLRERTGLSEVYTARGPSGALCRLSFF
                     HIDPDGLPWKRFLAESQQVRALSHPCLAEVAETAVSSDGRPYQVLVLGDGEDLGTRLR
                     RGALTVDEALVCADQIGAALLALHSLDLSHRGLCPDRIFLSSPSDPVQPRVRILDAGV
                     TRLLDDSLPGVLAGNPEYKAPEQLSGLSIDVGPAVDQYTLALLLYQALTSSRPFKADS
                     PAATILQVVRGGAEPLRVLRPDVSRNLDAAMMRALGKERQSRFPSVAEFLTALRGGQE
                     VTPAIDALVGNWLRKSESAQAAIQNAQAQADSKPSFQVVALGMADASAGGVPEVVEDQ
                     ATVPNAMDEVMRLALPIEQVPLVTSSPVAAPKSGPVASSAPIVISSPIIVDSRPTALA
                     SGPAIASEKTDPQQVLSQAAPRSGISEQSTGPTSQSDRTDKTALAGPQPIAGPSQSLA
                     DGTAGPSTLLRSPMTERAVIALIGLVIGFLLRHLIG"
     gene            3451..>3974
                     /locus_tag="PKL17_20940"
     CDS             3451..>3974
                     /locus_tag="PKL17_20940"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47262.1"
                     /translation="MPEKASAIFLKTGVLTPQGLNAAIALKQRDGGTLSEAILRLGLL
                     DEEHLVSAFQKNMQIPRVSAAALARVSKDVLNAIPADLAAQLRVIPMDIDASGNLTLA
                     MVDPSDDDAVEQVIMHTLSEVNRAVASPTAMREALRTHYGVVLSKQGAPAAAPAAAAP
                     AAKSGPVVAPAPAAA"
BASE COUNT          736 a         1270 c         1218 g          750 t
ORIGIN      
        1 gaagcagcgc cgcttcttcc tggttgagct tgcccaggcg atcggcaatt tcttgcagcg
       61 ccgccagcag atccttggcc ggtttttcac cccgcgccga gtcctgctgc acccgcccga
      121 tcagcagcaa aaacgtttca cttttgcggt gggcagcctg cacccgatcg gcagcttccg
      181 tcgacaaatc ggtctggtct ttgggctccc gggtgagtcc gcgcaaaaga tcaatccgat
      241 cggccagaag ctgtaccgct tgctcgcgca gctgggactg gctttgcaac agcgcttcct
      301 gacgctgacg cgggctttgg ataaacagcg cgtattcccg tgaccgaccg acgcccggac
      361 cggtgacgtt gttgaggtcg cgggcctcgg caaaatatgt cacccgcacc cccggttgaa
      421 gctccagctc gctcaaatcc cattcgatct tggccgccaa gttgcgcggt acggtcgggg
      481 tgttgatgcc gtttttctgc gtcggtttgt cgtcgggttt gctgtcccca gtcggtgccg
      541 atcggagcct tcgtcgccgt tccggcccgc tgccaatccg atagaccaaa tcgatatcgc
      601 ccagaccaaa gtcatcctcg gcgctgtagg ccagctcgat ccgcctggtc gacgtcactt
      661 ccagttccgc ggcgggtgca aacagatcga tccgcggcgg atgatccgat tcgatcgcaa
      721 ttcggtggct ttgtccctcg cgcagcagcg ctttgtggcc atttttgatg acaaagtagt
      781 agctgcccgc tttttgcacc accaggctcg ccgacagaag cggcagcggc gcatcttgtc
      841 cggctttcgg tttgccgact tggaccggct ggctcgattc ctgaccgtcg gcgatcaccg
      901 tgatttgggc acgctcgacg ggaaccagcg cccacgcctc gatccgcact tgcgttcccg
      961 gcagcgccaa gatgtcgccc gtcgagccgg gaatcgttcg ctgatgcagc cctgtgtact
     1021 tggggtagac caaaagcaag cggatgtccc cgagcagcgg ctccgtcgcg atggacaccg
     1081 gctccggcgg cgacaccgtc cccagcgtgt gcagaccttt ggcgagcggc aacgctccag
     1141 ccagcgtcgc aaacagacca aatccaaaca gcatcagcgc gctcagaccg gcttgccaga
     1201 ccggccagcg atccaccaag ctgtctggct gcaccgtccc cagcgcagcc agcgtccgtt
     1261 gtgccagctc ttcgacgaga gcgcgctccg gtccgttcgt cagctctttt tgcagctcga
     1321 tggtcgacag cagttccgag cgcagatcgg gaagccgcgc gccgacaaaa cgcgccattg
     1381 cttcgtcgct ggttcgttcg cttcgggccc ggacttgcag caccgcgagc gcgagcaagg
     1441 ccaacagcgc atagaccccg cagatcggca gccacgccca gtgcggtccc gtctgcgccg
     1501 cgagcagcag caccgcggca ccacaaactc ccgtcgcctg cagccacaga agcaagagcc
     1561 gcagtttttc ccgccgcagc agccgtcgtc gaacctgcga tagataagac tgcagccgcg
     1621 caaacggcga cgtgacgatg ggcggaggcg tgatctcagc cgggctcgaa gggagcatgg
     1681 gctgagaatc gaacgcttgc gacatacgca gcattattcc caatttttgc gcgcctgacg
     1741 accccgcacg agagaggaag tccgaagaac gtgatcattg cttcccgaga atatttcctg
     1801 taatttatcc cacattatgg gaagagaatt gccgcaaggc acggctgtgg gtgcgtactc
     1861 gatcgagcga ctgcgcgaga ggactggtct tagcgaggtc tacactgccc gcggaccgtc
     1921 gggcgcgctg tgtcgtctga gctttttcca catcgatccc gacggactgc cctggaagcg
     1981 ctttttggcg gaatcgcagc aggtgcgggc gttgtctcat ccgtgcctgg ccgaagtggc
     2041 ggaaactgcg gtcagcagcg acggacgtcc ctatcaagtg ctcgtgctgg gagacgggga
     2101 agacctgggc acgcggctgc ggcgtggggc tttgaccgtc gatgaagcac ttgtctgtgc
     2161 cgatcagatc ggggcggcac ttttggcgct gcattcgctg gatctgtcgc accgggggct
     2221 ctgtcctgat cggattttcc tgtcgagccc aagcgatccc gtgcagccgc gggtgcggat
     2281 tctggatgcc ggcgtgacgc gactgctcga cgattcgctg ccgggcgtct tggccggaaa
     2341 cccggaatac aaagcgccgg agcagctcag cgggctgtcc attgatgtcg gtcctgccgt
     2401 cgaccagtac accctggcgc tgcttttata tcaagcgctg accagcagtc gaccgttcaa
     2461 agcagattcg ccggcagcga cgatattgca agtggtgcgg ggtggggccg agccgctgcg
     2521 tgtactgcgt cccgatgtca gtcggaacct cgacgcggcg atgatgcggg cgcttggcaa
     2581 ggaacgacag tcgcgctttc catcggttgc tgagtttctg acggcgctgc gaggcggcca
     2641 agaagtgacg ccggcaatcg atgcgctggt cggcaactgg ctgcgcaaaa gtgaatcggc
     2701 gcaggcggcg atccaaaacg cgcaagcgca ggccgattcc aagccttcgt ttcaggtggt
     2761 cgcgctcggg atggccgatg ccagcgctgg gggcgttcca gaagtcgtcg aagatcaagc
     2821 caccgtgcca aacgccatgg atgaagtcat gcgtctggcg ctgccgatcg aacaagtgcc
     2881 gctcgtgact tcgtcacccg tcgctgcgcc aaagtcaggg cccgtggctt cgagcgctcc
     2941 gatcgtgatc agcagcccca tcatcgtcga ttcccggccc actgctttgg cgtctgggcc
     3001 cgcgattgca tcggaaaaga ccgatccaca acaagtgctt tcgcaagcgg caccgcgatc
     3061 gggaatttcc gagcagtcga ccggcccgac ttcgcaatcg gaccggaccg acaaaaccgc
     3121 gctggcgggt ccacagccga tcgccggacc atcgcaaagc ctcgcagatg gaacggctgg
     3181 cccgtcgacg cttttgcgat cacccatgac cgaacgcgcc gtgattgcgc tcattgggct
     3241 tgtcatcggc ttcctgcttc gtcacctgat cggttagctc atgaccggtt ggccgacgcc
     3301 agaatgacct acccacagtc agccgatcgc caagccgcaa aatcccgaaa aatcgcaagt
     3361 tgcgcggccc cactttcgcg ggccgtacca ccgagggaag ctccggtgag cgcttgactt
     3421 tccgctcccg cagtgggaag taaacatact gtgccagaga aagcatctgc catattcctc
     3481 aagaccggtg ttctaactcc ccaaggactc aacgcggcca ttgcgctcaa gcagcgagac
     3541 ggcggcacgc tcagtgaggc gatccttcgg ctcggcctcc tggacgaaga gcacttggtt
     3601 tccgcgttcc aaaagaacat gcagattccg cgtgtgtcgg cggcagcgct ggcgcgggta
     3661 tcaaaggatg ttctaaacgc gattcccgct gacttggcgg cgcagctgcg ggtcattccg
     3721 atggacattg atgccagcgg aaacttgacc ctcgcgatgg ttgacccgag cgatgacgac
     3781 gccgtcgagc aagtcatcat gcacacgctc tcggaagtca atcgcgcggt tgcgtcacca
     3841 acagcgatga gagaagcgct gcgaacgcac tacggcgtcg tgctgagcaa gcaaggcgcc
     3901 cccgccgcgg ccccagctgc ggcagcgcct gcggccaagt cgggtccggt tgtcgcgcct
     3961 gcgcccgcag cggc
//
LOCUS       DAORGN010000313         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12594_length_3959_cov_5.507428, whole genome shotgun sequence.
ACCESSION   DAORGN010000313 DAORGN010000000
VERSION     DAORGN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_3959_cov_5.507428"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKL17_20945"
     CDS             complement(<1..564)
                     /locus_tag="PKL17_20945"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNK47263.1"
                     /translation="MQKAQRANGLGTKGFGLAAGLCFTTLIGCGGAELGGEPENLGPY
                     AEQGEWHEYMAAATDTVADAADSGACSTAAIRGLSDQIVFQMNCIRSGLMTRIDGYNV
                     SFASAAALPYLQTPAATALNRATTSRSRLSLNSTLRSVAQQYMLYHWYKTGTCGVQLA
                     AAPGQSNHEDGMALDTSDYSAWRGALTG"
     gene            741..2183
                     /locus_tag="PKL17_20950"
     CDS             741..2183
                     /locus_tag="PKL17_20950"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNK47264.1"
                     /translation="MRKPPFHPIHPLKWTVGLLPGLCAGVWGLLAGCQPTLDTRTALI
                     VQTLVDADRDLIANRPALLATKYEVMAEGLQPFLRGTAGIFYRDLSRYQDEAAQVPHG
                     VAAEQVQLYGDIHLENVGVTFDTSGVLFDCVDFDSTVQGPFGWEVRRTALSLRAALSL
                     SGQPDAVLDEVVARLGQSYAQTMRDRSQQSADTNTQIRESSPQAGKIVLELLADGKKR
                     NDQQEELSQYTEVVSKTRKLLRNADLQDMPSPWAGDLPQLIAAYRDTRRSGRGQDSQY
                     QILDAAQRFNSGVASLPNLRFWVLLSGPNPNGNPMRNDPEWILEFKEERDPPLPLGWL
                     GRGPIGKNDARVLAGTRLLLASPDSEADLGAVRFAGVSFQVRRVLRGRRDLDVAKLTE
                     RTLSGRYGVADLKDLAATLGGLIANGHARAGHPLALWNVLAKSDGTGDGFTAYLVDQS
                     RSDVERLGRDFVLFQAARDLLGPLLGARQN"
     gene            2193..3344
                     /locus_tag="PKL17_20955"
     CDS             2193..3344
                     /locus_tag="PKL17_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47265.1"
                     /translation="MIEKQPRHLKTRWLGPVALAMPLLSQSPVASAHPMRPETGLPYS
                     AEAKEKLPEKTPASPGQLQGSIGPLRLGIVGDLFATYRLVDRQDQVFHEFELSRIQLS
                     GWASYEELAGVNITVDTVRSSSNRSYQGVDGDAILPRLKWAFAEATPWKHYVALRAGI
                     VPDLLLQYAEASWGYRVQGPTGLERDALYTPGDLGASVEVALPWQLGAAAIQVGNGEG
                     ISLREQNNGKNLTGVLRLAPLRSQAPDLLIHLLFRDGSIGAGTAADRRGSVGITYASP
                     KLGAGAVGTLALGYRGIGDRFAGHLSTWLRGELPACFGLLGRIDALWPDVSSRQDVQL
                     RVIGGVSYSLPSLVRLIASYEGTVPFGGLAGQVPSITEHAMLIQAEVRL"
     gene            3341..3946
                     /locus_tag="PKL17_20960"
     CDS             3341..3946
                     /locus_tag="PKL17_20960"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNK47266.1"
                     /translation="MTQHFAVYVALVGCAGCSLANTVPPPDDVQWRTDLAGSGGTPSD
                     AGSGKDGSSDLARPGPPSTLVVNEVYPHGSDVMTDPDFVELFNGGSGQVNLRGYKVRD
                     HNATWYALPDDAMIPAGGFYIINCDGLMSGALPGAHVPFKLGGSGDEVHFAGPDGIEL
                     DSATWGTGIIDVPKNQSLGRVPDQTGPFVVLTKPSRGKSNQ"
BASE COUNT          749 a         1168 c         1267 g          775 t
ORIGIN      
        1 cccggtcagc gcaccgcgcc atgccgagta gtcggacgta tcgagcgcca tgccatcttc
       61 atggttcgat tgtcccggtg ccgccgcaag ctgcacgcca catgttccgg ttttgtacca
      121 gtggtagagc atgtactgct gggcgacact gcgcagggtg gaattgagcg acagccgact
      181 gcgcgacgtg gtggcacgat tgagcgcagt tgccgccggg gtttgcagat aaggcagcgc
      241 cgcagcggag gcaaagctga cgttgtagcc atcgatccgc gtcatcaggc ccgaacgaat
      301 gcagttcatc tgaaagacga tttgatcgga cagaccccga atcgccgcgg ttgaacacgc
      361 gccactgtcc gcagcatcgg ccaccgtgtc tgtcgctgcg gccatgtatt cgtgccactc
      421 gccttgctcg gcataaggac caaggttttc tggttcgcca cccagttccg ctcccccaca
      481 gccgatcagc gtggtgaaac agagtcccgc ggccagtccg aatccttttg ttccaagtcc
      541 gtttgcccgt tgcgcttttt gcatcacttt tggactccgt gtacgagggg ggaaactcgg
      601 tctcctatcg gtatgtaccg cagtccgcgc gcgccggagg caacaatctg tgacaggaat
      661 gtgacttctt ctgcccaaat tcagttggat tgtttcgatc atcgccaaac tgggttaagc
      721 acagacgaag cgaggtctgt ttgcgtaagc cccctttcca tcccattcac ccgttgaaat
      781 ggaccgtcgg cctgctgccc ggtctgtgtg cgggcgtttg gggcctgctt gccggctgtc
      841 agccgacgct cgacacccgc acggcactca tcgtccagac acttgtggac gccgaccgtg
      901 atctgattgc gaatcgccca gcgcttttgg cgaccaaata tgaagtgatg gccgagggac
      961 ttcagccctt tttgcgcggc acggcgggca ttttctatcg cgacctgtcg cgctatcagg
     1021 atgaagcagc ccaggttccg cacggggtcg ctgccgagca agtacagcta tacggagaca
     1081 tccacctgga aaacgtcggc gtgacgtttg acacaagcgg cgtgctgttt gattgcgtcg
     1141 actttgattc cacggtgcaa ggtccgttcg gctgggaagt gcggcgaacg gcgctgtctc
     1201 tgcgcgcggc gctgtctctg tcgggacaac cggacgcggt gctggatgaa gtcgtggcgc
     1261 ggctcggcca aagctatgcc cagacgatgc gggatcggag ccagcagagc gcggacacaa
     1321 atacgcagat tcgcgagtca agcccgcagg ccggaaagat cgtgctggag ctgctggccg
     1381 atggcaaaaa gcgcaacgat caacaggaag agctgtcgca gtacacggaa gtggtgtcaa
     1441 aaacccgcaa acttctgcgc aacgccgact tgcaggacat gccaagtccg tgggccggcg
     1501 atctgccgca gctcatcgcg gcgtaccgtg acacccggcg cagcggacgc ggccaggatt
     1561 cccaatatca aatcctggat gcggcgcagc ggttcaactc gggggtggcg tcgctgccaa
     1621 acttgcgctt ttgggtgctt ttgtccgggc caaatccaaa cggcaacccg atgcggaacg
     1681 acccggagtg gatcttggaa ttcaaagaag aacgcgatcc gccgctgccc ctgggatggc
     1741 tcggacgcgg gccgattgga aaaaacgatg cacgggtgct ggctgggacg cgcctgcttt
     1801 tggccagtcc cgatagtgaa gcagacctgg gagcggtgcg ttttgccggg gtgtcctttc
     1861 aagttcggcg cgtgctgcgg gggcggcgcg atctggatgt cgcaaagctc accgaacgca
     1921 cgctatcggg ccgctatggc gtcgctgatt tgaaagacct ggcggccacg ctcggtgggc
     1981 tgattgcaaa tggacatgcg cgtgccggac atcctttggc gctgtggaac gtcctggcca
     2041 aaagcgatgg cacgggcgat ggattcactg cgtatctcgt cgaccaatcg cgaagtgatg
     2101 tggaacgact cggcagggat tttgtgctgt tccaggccgc gcgtgacctt ttggggccgc
     2161 ttctgggcgc ccgacagaac taggaaacag gaatgatcga aaaacaaccc aggcatctga
     2221 agacccgctg gcttgggccg gttgcactgg ccatgccgct gctgagccaa agccccgtcg
     2281 cgtctgccca tccgatgcga cccgagacgg gcctgccgta ctctgccgaa gcaaaagaaa
     2341 agctaccgga aaagacccct gcaagcccag gtcaactgca aggcagcatc ggcccactgc
     2401 gactgggaat cgtgggcgat ctgttcgcga cgtaccgtct ggtcgaccgc caagatcagg
     2461 tgtttcacga gttcgagctg tcgcgcatcc agctttcggg ctgggcaagt tatgaagagc
     2521 tcgcgggcgt aaacatcacg gtagatacgg tgcgatcgtc gtcgaatcgg tcgtatcaag
     2581 gggtggatgg ggatgcgatc ttgccgcggc tcaagtgggc gtttgcggaa gcgacgccgt
     2641 ggaagcacta cgtcgcgctg cgtgccggca ttgttcctga tcttttgctg caatacgccg
     2701 aggcgtcgtg gggttatcgc gtccaaggac cgacgggact ggagcgcgat gcgctgtaca
     2761 caccgggcga tctgggcgct tcggtcgaag tggccctgcc gtggcagctg ggcgcggcag
     2821 cgattcaggt tggcaacggc gaaggaatct cactgcgcga gcagaacaac ggcaaaaatc
     2881 ttaccggggt tctgcgcctt gcaccgctgc gctcgcaggc tccggacttg ctgattcatc
     2941 ttctgttccg cgatggttcg attggcgcgg gaacggcggc agaccggcgc ggcagcgttg
     3001 gaatcaccta tgcatcaccg aaactgggtg cgggtgccgt gggaacgctg gcactgggtt
     3061 atcgcggcat cggcgaccgc tttgcggggc acctgtccac atggctgcgc ggcgagctgc
     3121 cggcgtgttt tggtctgctt ggacggatcg atgcgctgtg gccggatgta agctcgcggc
     3181 aagatgtcca gcttcgcgtg attggcgggg tttcctattc gctgccgtcc ctggttcggc
     3241 tgatcgccag ttacgaagga accgtgccgt ttgggggtct ggcaggtcaa gtccccagca
     3301 tcacggaaca cgccatgctg atccaagcgg aggttcggct gtgacgcagc atttcgcagt
     3361 gtatgtagcg cttgttggct gcgccggttg ttcgctcgcc aacaccgttc ccccaccgga
     3421 cgatgtgcag tggcgcaccg atctagccgg gtccggcgga accccgagcg atgccgggtc
     3481 cggcaaagat ggcagcagcg accttgcgcg cccaggtccg ccatccaccc tggtggtcaa
     3541 cgaagtctat ccgcacggca gtgatgtgat gacggaccca gactttgtcg agcttttcaa
     3601 cggcggatcg ggacaggtca acctgcgcgg ctacaaagtg cgtgatcaca acgccacttg
     3661 gtatgcgcta cccgacgatg ccatgattcc ggcgggcggt ttctacatca tcaactgcga
     3721 cggcctgatg agcggcgccc tgcccggcgc gcacgttcca ttcaagctgg gcggcagtgg
     3781 cgatgaagtc cacttcgctg ggcccgacgg gatcgagctg gacagcgcga cgtggggcac
     3841 cggcatcatc gacgttccca aaaaccagtc gctcggcaga gtgcccgatc agaccggccc
     3901 gtttgtcgtc ctgaccaaac cgagccgcgg caagtcaaac cagtaaccac cccacctga
//
LOCUS       DAORGN010000314         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_12662_length_3947_cov_5.385663, whole genome shotgun sequence.
ACCESSION   DAORGN010000314 DAORGN010000000
VERSION     DAORGN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12662_length_3947_cov_5.385663"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKL17_20965"
     CDS             <1..780
                     /locus_tag="PKL17_20965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK47267.1"
                     /translation="IMGAFSVASVAGVPLGLWLARHGGFRTPFFVVAGLGLLILAFTF
                     VVLPPLRSHLQQLVPARSLTEILRRPVVRLALTTIAVSNMATFAIVPSMAPYLIRNCG
                     MRRTDLEYMYALGGACTFVSMRIVGWLVDRYGAARVMVLASLFLSGVLVTGFLPEHNL
                     LPITIVFVGFLISVSSRNVCVNATCSRVAAPSERARYQSLQSAVQHMASAVGAALGPI
                     FLHELADGRLGGIAPLSVLSIVLTLALPTLMYRIYRLGELH"
     gene            complement(787..3840)
                     /locus_tag="PKL17_20970"
     CDS             complement(787..3840)
                     /locus_tag="PKL17_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNK47268.1"
                     /translation="MVVSSLRLALGRSVRKGSGQPETICLRFPDAPPQSELFGELPHW
                     HKPNPLTPGPDGIPEVTLSLFPGVYAYKLRTSDGNWHLQSSNPRTRSCDGHRNHVLVI
                     GGTAEPILHAPVAPWVFGFRDGSLTIRAALRKSAANNLSLRLRTEESTSVLPMQPVGE
                     EDEHLLLEALVPTVGLAVQYWFVLPSGQLVGGPSGEAFCVEPQRPDIPSWWQDSVLYS
                     VFVDRFRRGDGGPWPVLADEKARAGGDLDGVCAALPYLSDLGVQVLHLTPIWQSPSAH
                     RYDAENPLVVDPALGGEAALRRLVAEATRLGIRILLDVTLTHVHRNFLPFCDVRQRGF
                     DSPYADFFRIYRWPFSDGQAPGYEHYQGGQWCEPLLNAENPDVATYLLRVLWHYLSFG
                     VAGFRLDSAADVPPALLAVLHDGVRAKSKDALLLGELTVDNLGHYVGYGLDCATDFAL
                     RTGLCDWLSSRLRASQFRALCQQRSFVRGPSHAAVGFSATHDVPRLRTFVDEKRALIG
                     HLFSLLRPEVPMIYAGDELGLHSDEPGRRFEDVWPDRQPFPWSETERQNPTLRLFHSV
                     LSLRHAHAAIRRGSCLDLPLAEAPDVLAFRRQLGDEIVDVYLHPRDTVVEFALPEGPS
                     ELEPLLLVGEADRDATTQTVRLGPCSAWVLLRAVPRELEALSTAIVDHAEHRSREAFR
                     LGQTAGLLLPSKLYLTVTERCNLRCQHCITHAPEKTLRRTARSMQPWLFDALDEALQA
                     ASYFGFSHGGESLLSPDFEKLLKRIRSARHGRPYDVHLLSNGMLLHPDKIEQLVELGV
                     SSLAVSLDGGTAETNDEIRAGCDFEKVLHNLRHAVGLRRAQNLDLRIGVSTVVLRQNI
                     DELPVLAQLVCELGLDWLKLEEGYPANLFSAQSLLRPDGNRVREAVKKVRAIVEPNGV
                     ILVDHLAPPSGCPCQATLGDALSEFRATDDFANRTCFRPCRAAWDIACVDPDGAVHVG
                     DYASKPIGSLFTTSFVDLWNSPKAQAHRRDALAQIDPALRAACPH"
BASE COUNT          773 a         1278 c         1180 g          716 t
ORIGIN      
        1 atcatggggg ccttttcggt cgcgtcggtc gccggcgttc cgctcgggct gtggctggcg
       61 cggcacggag gcttccgcac cccatttttc gtcgttgctg gacttgggct gctcatcctt
      121 gcgttcacgt ttgtggtgtt gccgccattg cgctctcact tgcagcagct tgtgccggcc
      181 cgcagcctga ccgaaatcct gcgccgcccg gtcgtgcggc tcgcgctgac caccatcgcc
      241 gtcagcaaca tggcgacgtt cgccatcgtc cccagcatgg cgccgtacct gattcgcaac
      301 tgcggcatgc gccgaaccga tttggaatac atgtacgcgc ttggtggtgc ctgtacgttc
      361 gtttcgatgc gcatcgttgg ctggctcgtc gatcggtacg gagccgcgcg ggtcatggtg
      421 ttggccagcc tgtttttgtc gggagtgctc gtcacaggtt ttttgcccga gcacaacctc
      481 ctgcccatca ccatcgtatt cgtcggtttt ttgatcagcg tgtcgtctcg caacgtctgc
      541 gtaaacgcca cctgcagtcg cgtcgccgcc ccgagtgagc gcgctcgcta tcagtcgctg
      601 cagtcggcgg ttcagcacat ggcgtccgcc gtgggagccg ctctcggtcc gattttcctg
      661 cacgagctgg ccgacggacg gctgggcgga attgcgccgc tctcggtgct gtccatcgtg
      721 cttacgctgg cgctgccgac gctgatgtat cgaatctatc gactcggcga gctacactaa
      781 ccgccgtcag tgcggacacg cggcacgcag cgccggatcg atttgtgcga gcgcgtcccg
      841 acgatgggcc tgcgctttcg gactgttcca caagtcgaca aacgacgttg taaaaagcga
      901 tccaatcggc ttgctcgcgt agtcgccgac atgcaccgct ccgtcgggat ccacgcacgc
      961 gatgtcccaa gcggcacggc acggacgaaa gcaggtgcgg ttggcaaaat cgtcggtggc
     1021 ccgaaattcg gacagtgcat caccaagcgt tgcctgacac gggcagccgc tgggtggcgc
     1081 cagatggtcg accaaaatca cgccgtttgg ctcgacgatg gcacgtactt ttttcaccgc
     1141 ttcacgcacc cgatttccgt cgggacgcag cagcgactgc gccgaaaaca ggttggccgg
     1201 atagccctct tccagtttta gccagtcgag cccaagctcg cagacgagct gcgccagcac
     1261 cggcagttcg tcgatgtttt gccgcagcac caccgtcgag acgccgatcc gcaagtccag
     1321 gttttgtgcc cgtcgcagcc ccaccgcatg ccgcaggttg tgcagcactt tttcaaaatc
     1381 gcagccggcg cggatctcgt cattggtttc cgccgtccca ccgtcgagcg acaccgccaa
     1441 gctactgacc cccagttcga cgagctgctc gattttgtcc gggtgaagca gcatcccatt
     1501 tgacaaaaga tgcacgtcgt aaggtcgtcc atggcgggcg ctgcgaatcc gcttgagcag
     1561 cttttcaaag tcgggggaca gcaggctttc gccgccgtgg gaaaaaccaa aatagctcgc
     1621 cgcttgcagc gcttcatcaa gcgcgtcgaa cagccacggc tgcatcgacc gtgccgtccg
     1681 tcgcagcgtt ttttccggcg cgtgggtgat gcagtgctgg cagcgaaggt tgcagcgctc
     1741 cgtcaccgtc agatacagct tcgatggcag aagcagccca gcggtctgcc ccagtcgaaa
     1801 tgcctcgcgg ctgcgatgtt ccgcgtggtc gacaatcgcc gtcgagagcg cttctagctc
     1861 gcgcggcacc gcgcgcaaca acacccacgc cgaacacgga ccgagccgca ccgtctgcgt
     1921 cgtcgcatcg cggtcggctt cgcccacaag caaaagcggc tcaagctccg acgggccttc
     1981 gggaagtgca aactcgacga cggtgtcccg cgggtgtaga tacacatcga cgatttcgtc
     2041 accgagctgt cgacgaaacg ccagcacatc gggggcttcc gcaagcggca gatccagaca
     2101 gctaccccga cggatcgccg catgcgcatg gcgcagcgac agcacggaat gaaacagccg
     2161 cagcgtcgga ttttggcgtt cggtttccga ccacggaaac ggctgccgat ccggccagac
     2221 atcttcgaag cgacgtcctg gctcgtcgct gtgcagaccc agctcgtcgc cggcatagat
     2281 catcggaact tcgggacgca aaagcgaaaa caggtggcca atcagcgctc gcttttcgtc
     2341 gacgaacgtc ctcaaccggg gcacatcatg ggtcgccgaa aaaccgaccg ctgcatggct
     2401 cggtccgcga acaaagctcc gctgctgaca cagcgcacga aactgcgacg cacgcaaccg
     2461 actcgaaagc cagtcgcaga gcccagtccg cagtgcaaag tcggtcgcac aatcgagacc
     2521 atatccaacg taatgtccca ggttgtcgac tgtcagctcg ccaagaagca gggcatcttt
     2581 cgatttggcg cgcactccgt cgtgaagcac cgcgagcagc gccggaggca catccgccgc
     2641 tgaatcgagc cgaaagcccg caacgccaaa cgaaaggtag tgccatagca cgcgcaaaag
     2701 atacgtcgcg acgtcgggat tctcggcatt gagaagtggc tcgcaccact gaccgccttg
     2761 atagtgctcg tagccgggtg cctgcccatc ggaaaagggc cagcgataga tccggaaaaa
     2821 gtccgcataa ggcgaatcga atccgcgctg cctgacgtcg caaaacggca agaagttgcg
     2881 atgcacatgc gtcagcgtca cgtcgagcaa gatccggatg ccaagccgcg tcgcctctgc
     2941 aaccagtcgg cgcagcgccg cttcaccacc gagcgccgga tcgacgacga gcggattttc
     3001 cgcatcgtag cgatgggccg acgggctctg ccaaatcggt gtcaggtgca gaacctgcac
     3061 gcccagatcg gacagatacg gcagcgccgc gcaaactcca tcgagatcgc cgcctgcgcg
     3121 cgccttttca tcggcaagaa caggccacgg gcccccatca ccacgtcgga accgatcgac
     3181 aaacaccgaa tagaggacag aatcttgcca ccacgatggg atgtcgggtc gctgcggctc
     3241 gacacagaat gcttctccgc tgggcccccc cacaagctgt cccgacggca aaacgaacca
     3301 atactgcacg gccagcccca ccgtaggcac cagcgcttcg aggagaagat gttcgtcttc
     3361 ttcgcccacc ggctgcatcg gaagaaccga cgtcgattcc tcggtacgca gccgcaggga
     3421 cagattgttc gccgctgact tgcgcagcgc ggctcgaatc gtcaggctgc cgtcgcgaaa
     3481 accaaaaacc cacggtgcca caggcgcgtg caggatcggt tccgccgttc ccccgatgac
     3541 gagcacatgg ttgcgatggc cgtcacaact gcgggtgcgc ggattgctgc tttgaaggtg
     3601 ccagtttccg tcggaagtcc gcagtttgta ggcatagacg ccgggaaaga gcgaaagcgt
     3661 gacctcggga attccgtcgg ggccgggtgt gagcgggttt ggcttgtgcc agtgcggaag
     3721 ctcaccgaaa agctcgctct gcggcggcgc gtcgggaaaa cgcagacaga tcgtttcggg
     3781 ctgccccgac cctttgcgga cggatcgccc caacgcaagc cgcagcgatg acaccaccat
     3841 caggtcgacg cagcgtcgag cggcgcggca tgaagaccag cagccttgag catcgcttga
     3901 tcgtcatcgc aatcgggatt gcccgtcgtc agcagcttgt gcagctg
//
LOCUS       DAORGN010000315         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_13140_length_3858_cov_5.757034, whole genome shotgun sequence.
ACCESSION   DAORGN010000315 DAORGN010000000
VERSION     DAORGN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13140_length_3858_cov_5.757034"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PKL17_20975"
     CDS             <1..686
                     /locus_tag="PKL17_20975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK47269.1"
                     /translation="LAINDMAGRIEKQLKDQRELLAAVSHELRTPLTRIRLLLEMARD
                     GSEKHLDEMDQEIVEMDRLVGELLAQARLDFSALSRRTISAQDVAARALERAAIADGK
                     LVAVDVLLSADATLLSRALLALLDNAKKHGAPPIVLRVERLSDSVRFSVEDSGAGFPS
                     GEEARAFEAFRRGAQDGGGLGLGLSLVKRIAEAHGGQVFAHNRTEGGAVVGLQLPIGI
                     ADEKSSDGD"
     gene            complement(683..1456)
                     /locus_tag="PKL17_20980"
     CDS             complement(683..1456)
                     /locus_tag="PKL17_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47270.1"
                     /translation="MSAEQTPPDSPKPPSPKESAASTPSSASQVPSKDTPSPRAKPRI
                     SSLLDVIRGERPSGSSPLPSQLSPRPSGLTPSPSQLSPRPSQLSPKLAPKRHISQVIE
                     TLKLPNRDIAERLVRLGFDSETACVLHVLPLIQVAWANNDISRRERSRILHVLKLREI
                     PEGSRAWVMVESMLETRPTDAFLHACREVLRDMLATSKSGPGQEERQLIDLCVEIAEA
                     SGGLFGLVRAVSAAEKTVIADIAATFGDDAQQEMKRLLS"
     gene            complement(1587..2072)
                     /locus_tag="PKL17_20985"
     CDS             complement(1587..2072)
                     /locus_tag="PKL17_20985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNK47271.1"
                     /translation="MSLRMLPSFRPLFALPVLGLVGIAVAQPAAPAQKPSQSPAPAAA
                     PQGAQMVTTKSGLKYIDQQVGTGPVPQVGKTVRVHYTGKLANGQKFDSSVDRGEPFEF
                     QIGVGQVIKGWDEGVLSMKVGGKRTLIIPASLGYGARGVGGVIPPNAELHFDVELLGV
                     K"
     gene            complement(2094..3359)
                     /locus_tag="PKL17_20990"
     CDS             complement(2094..3359)
                     /locus_tag="PKL17_20990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK47272.1"
                     /translation="MRLSPASEQYRTARWPVLLTFSLFATLGLFGCRAKTQTVAPPNP
                     EKVAAQQSLPKPKAPFVPRKLPRIDVHTHLLLGTSLRAARLFQKYGISLAVNLSGAPG
                     GHGFEEFPVDNEVAYGHIVAFTTLDFEETASAGYGQRMADSLVEAQKSGARGLKIPKG
                     LGLGYKGPDGKLLKVDDPGLDAVFEKAGALKLPVAIHVADPQAFWQEPGPKNERFDEL
                     LAHPEWSFYDAQKRGEVPSWRELFDAFLRRVARHPKTIFIGVHFGNAPEEPDLVAQAL
                     DKYPNLYIDTAARLPAIGRSDQNHSATKLRAFFIKYQDRILFGTDTAFGRNGLPAMFG
                     SVGTDAPTVADADRFFDSTYRYFETDDTDIPSPTPIQGKWNIAGINLPVEVLEKIYFR
                     NAEKLLGVTLPQKALPQIEQGAIPTGDDL"
     gene            3498..>3858
                     /locus_tag="PKL17_20995"
     CDS             3498..>3858
                     /locus_tag="PKL17_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47273.1"
                     /translation="MNHRSLASLVRGSWRLLVLCGLVLGLVDSSVARAEGVKVHLESV
                     AIDKLPELRAYVSVVDEDSKPIRSKSGFKLQMDGNPLTDLTTTVQSFAESKEPMDVFV
                     VVQVSSVMKDGLGAAKTG"
BASE COUNT          709 a         1097 c         1252 g          800 t
ORIGIN      
        1 cgctggcgat aaacgacatg gcgggtcgga ttgaaaaaca gctcaaagac cagcgcgagc
       61 tgttggcggc ggtgtcgcac gagctgcgga cgccgctgac gcgaatccgc ctgcttctgg
      121 aaatggcgcg cgacggaagc gaaaaacatc tcgacgagat ggatcaagaa atcgtcgaaa
      181 tggatcgtct ggtcggcgag ctgttggcac aagcgcggct tgatttttcc gccctgtcgc
      241 gccgaacgat aagtgcccaa gacgtggcgg cgcgggcgct cgaacgagca gcgattgcag
      301 acggaaaact ggtggctgtg gatgtgctgc tgagcgccga tgcgacgctt ttgtcgcggg
      361 cgcttttggc cctactcgac aatgcaaaaa agcatggcgc gccaccgatt gtgctgcgag
      421 tcgagcgatt gtctgattcg gtgcggtttt ccgtcgaaga cagcggcgct ggttttccct
      481 ctggtgagga agcgcgggca tttgaagcat tccgtcgtgg cgcgcaagat ggcggcgggc
      541 ttgggctggg tctgtcgctg gtgaagcgaa ttgccgaagc ccacggcggt caggtgtttg
      601 cccacaatcg cacagaaggt ggcgcagtcg tcggtttgca gttgccgata gggatcgctg
      661 atgaaaagtc gagcgacggc gattaactga gcaggcgctt catttcttgc tgggcgtcgt
      721 cgccaaacgt cgcggcgatg tcggcgatga cggtcttttc cgcagcactg acggcgcgca
      781 ccagaccgaa cagtccgccg gatgcctcgg cgatttcgac gcacagatcg ataagctggc
      841 gttcttcctg gccggggccg gacttgctgg tcgccagcat gtcgcgcagc acttcccggc
      901 aggcatgcaa aaatgcatcg gtcgggcggg tttccagcat cgattcgacc atcacccagg
      961 cgcggctgcc ttcggggatt tcgcgcagct tcagaacgtg caggatgcgg ctgcgttcgc
     1021 gccggctgat gtcgttgttg gcccacgcga cttggatgag cggcagcaca tgcagcacgc
     1081 acgccgtttc ggaatcgaaa cccagccgca ccagccgttc cgcgatgtcg cgattgggca
     1141 gcttcagtgt ttcgatgacc tgcgaaatgt gccgcttcgg tgcaagtttc ggcgaaagct
     1201 gcgatgggcg cggtgaaagc tgcgacgggc ttggcgtaag tcccgacgga cgcggcgaaa
     1261 gctgcgacgg cagcggtgag ctacccgacg ggcgttcgcc acgaatcaca tcgagcagcg
     1321 acgaaatgcg tggttttgcg cgcggcgacg gggtgtcctt ggatggaacc tgcgacgcgc
     1381 tggacggtgt ggatgctgcc gattccttcg gcgagggggg cttgggcgaa tcaggagggg
     1441 tttgttcggc gctcatctgt acgctccgtc gtgcgcacgc agggttactc gccttgcagc
     1501 gtaacctacg gggagcgctc acctcaacgg cgaagcgtgc tcacgtcgga gaccgcgacc
     1561 gatgggccaa tcattcaacc tgtgcgctat ttcacaccga gcagttcgac gtcaaagtgc
     1621 agctcagcgt tcggcggaat cacgccaccg acgccgcgcg ccccatagcc gaggctggcc
     1681 ggaatgatca gcgttcgctt gccgcccact ttcatcgaca gcacgccttc gtcccagcct
     1741 ttgatgacct ggccgacgcc gatctggaac tcgaatggct cgccgcgatc gacggagctg
     1801 tcgaactttt ggccgtttgc gagcttgccg gtgtagtgga cgcgcacggt cttgccgacc
     1861 tggggcacag ggccggtgcc gacttgttga tcgatgtatt tcagtcccga tttcgtggtg
     1921 accatttggg ctccttgcgg tgcagccgcg ggggctgggc tttgggacgg cttttgcgct
     1981 ggggctgccg gctgtgcgac ggcaattcca accaggccga gcacgggcag cgcaaagagc
     2041 ggacggaaac ttggcaacat gcgtagcgac atacggacct ctttgggggg ttcttacaga
     2101 tcgtcacccg tgggaatcgc gccttgttcg atttgcggca gcgctttttg cggcagcgtc
     2161 accccgagca gtttttccgc gttgcggaag tagatttttt ccaagacctc aacggggagg
     2221 ttgattccgg cgatgttcca tttgccctga atcggcgtcg ggctgggaat gtcggtgtcg
     2281 tcggtttcaa agtatcgata ggtcgaatcg aagaaccgat cggcatccgc taccgtcggt
     2341 gcgtcggtgc caaccgatcc gaacatcgcc ggcaagccat tgcgaccaaa cgcggtgtcg
     2401 gtgccaaaca gaatccggtc ttggtatttg atgaaaaatg cgcgcagctt cgtcgccgag
     2461 tggttttgat cgctgcggcc aatcgccggc agtcgcgccg ccgtatcaat gtataggttg
     2521 ggatacttgt cgagcgcctg cgccaccagg tcaggctcct cgggcgcgtt gccgaaatga
     2581 acaccgatga agatcgtctt gggatggcgc gcgacccgac gcaaaaatgc atcgaatagc
     2641 tcgcgccatg acggaacttc gccgcgtttt tgcgcatcgt agaagctcca ctccgggtgg
     2701 gcgagcagct cgtcgaatcg ttcgtttttc ggccccggtt cctgccaaaa cgcctgcgga
     2761 tcggcgacgt gaatcgcgac gggcagcttc agcgctccgg ccttttcaaa caccgcatcg
     2821 agccctggat cgtcgacttt gagcagcttg ccgtccggcc ctttgtagcc aagccccaga
     2881 cctttgggga tcttgagccc gcgtgccccg gatttctgcg cttcgacgag agaatcggcc
     2941 atccgctggc catagccggc gcttgcggtt tcctcgaaat cgagcgtggt aaaggcgacg
     3001 atgtggccat atgcgacttc gttgtcgacg ggaaattcct cgaacccatg cccacccggt
     3061 gcgcccgaca ggttcaccgc caagctgatg ccgtactttt gaaacagccg cgccgcccgc
     3121 aagctggtgc caagcagtag atgggtgtgg acgtcgattc gcggcagctt gcgcggcaca
     3181 aagggcgctt tgggtttcgg caggctttgc tgggccgcga ccttttcggg attgggcggt
     3241 gccaccgtct gggttttcgc ccgacaaccg aacagcccaa gcgtcgcgaa aagcgaaaac
     3301 gtcaaaagga ccggccagcg cgccgtccga tattgttccg aggcaggtga gagtcgcacc
     3361 ggcgcgagtt tagcaaaagg caagacccac aacatccctg tgctgcccaa ttcgcgaatc
     3421 cttggctggt tttgtggccc gctttcgccg tatgatggat cgggcaaatt tctattttgc
     3481 cgtctggaga gggccttatg aaccatcgct cgcttgcctc gctcgtccgc ggatcttggc
     3541 ggcttttggt gctgtgtggg cttgttttgg ggcttgtcga ttcgtcggtc gcgcgcgccg
     3601 agggggtgaa agttcacctg gaaagcgtcg ccatcgacaa actgcccgag ctgcgcgcct
     3661 atgtcagtgt cgtcgatgaa gacagcaaac cgatccgcag caagagcggt ttcaagctgc
     3721 aaatggacgg caatccgctg accgatttga cgacgacggt gcagtcgttt gccgaatcca
     3781 aagagccgat ggatgtgttt gtcgtcgtcc aggtttcgag cgtgatgaaa gatgggcttg
     3841 gagcggcgaa aaccggca
//
LOCUS       DAORGN010000316         3720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_13986_length_3720_cov_6.279127, whole genome shotgun sequence.
ACCESSION   DAORGN010000316 DAORGN010000000
VERSION     DAORGN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13986_length_3720_cov_6.279127"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKL17_21000"
     CDS             <1..271
                     /locus_tag="PKL17_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNK47274.1"
                     /translation="LAVEWSPHRIRVNAVAPGIIRTSGTDRYPPELLEVARRATPWKR
                     LGLAEEVAHLVTYLASPAADFVTGSTMYIDGGASLWGDMFPLPDE"
     gene            312..1928
                     /locus_tag="PKL17_21005"
     CDS             312..1928
                     /locus_tag="PKL17_21005"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNK47275.1"
                     /translation="MATFSLDRKLWGWGSALYQVPAPLLDLARAFLSARLCCHIPHAM
                     PVPTLDSIRMPNPRRAIPHDLAALVTTDRYHRATHTYGKAYRDVVRALDGDFSPAPDF
                     VAYPQNEADIERILRIARREHWAVIPYGGGSSVVGGIEPDAATRNRFSAVLSLDLCAL
                     DQLLQVDRASRCARIQAGAYGPSIEQQLKPHGYTLRHFPQSFEFSTLGGWIATRAGGH
                     FATLYTHIDDLVESLRMVTPQGTLETRRLPGNGAGPQQERLLCGSEGAFGVISEAWLR
                     VQELPQHRASCIVRFPSFALGAQACRALSQSGLFPANARLIEADEALFMGIGDGTQHL
                     LLLGFESAQFSQQDKLQAAVALCSQAGGTAGRAKIRSGDSELEDDTAAQWKQSFVRAP
                     YLRDELCRMGLLCETFETCTTWSHFPKLDEAVRAAAQTALAATCGTGLVTCRFTHLYP
                     DGPAPYYTVIAQGTPGQQVAQWDAIKAAVSTALVENGATITHHHAVGKDHRSYYLRQG
                     DPLAIAMLRAAKTAVDPTGIMNPGTLLPTD"
     gene            1972..2349
                     /locus_tag="PKL17_21010"
     CDS             1972..2349
                     /locus_tag="PKL17_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNK47276.1"
                     /translation="MEAAPPHIRAMARASLALDDDKLMAECDVDLFVASGPGGQHRNK
                     TESAVRLTHRPSGIVVTATERRSQIQNRIVALARLREKLEKLTFVPRPRRPTKPTRSS
                     QERRLSEKKRDSQVKKQRRSDYD"
     gene            complement(2386..>3720)
                     /locus_tag="PKL17_21015"
     CDS             complement(2386..>3720)
                     /locus_tag="PKL17_21015"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNK47277.1"
                     /translation="TLKIELWKESDTRVIARVSVVERNKVVISNAAIELYKDIPQPKA
                     KGGAPAQAAQAQAAPAVLTSGDVFSAIGGYIGKNPDLVGSVGTVFQFRLKGPDSVWTI
                     DLKNGKGAVSAGTEPQPDCTLELSDSDFLDMTTGKADPNKLYFGGKLKIGGNIMASQK
                     LMFLKKIDRSEIEAALGKKGGSAPAQAPSAVDPATVLTSTDIFVAIKEYIADSPDLAK
                     QIGTSFLFKLSGPDSAYHIDLKNAPGALAEGVKDADVTLSLSNSDFLDMVLGKANPQK
                     LFMNGKLKIGGNVMASQKLEFLMKMDKKRVEKVVMARLGGGGGGGGGGGGKKDAPAAP
                     AQAMQTQTAQAPAIVAALRDKLAKQPGLVADIGGLLQFVVKSPDAAFAVDPSGKVTDG
                     KVDGAVTTLTISDEDLAALFAGKADVRSLYQHGKLRVDGKVTPAHHLAFLSK"
BASE COUNT          644 a         1142 c         1188 g          746 t
ORIGIN      
        1 cctggcggtg gagtggtcgc cgcatcggat tcgcgtcaac gccgtcgcac ccggcatcat
       61 ccgcacgagc ggcaccgatc gctatccgcc ggaacttctg gaagtggcgc ggcgggcgac
      121 gccctggaaa cggctgggtc ttgccgaaga agtggcgcac ttggtgacgt atttggcgag
      181 ccctgccgcc gattttgtga ccggatcgac gatgtacatc gatggcggtg cgtcgctgtg
      241 gggcgatatg tttccgctgc cggacgaata gttccgaacg tctcaaactg cgcgcggcaa
      301 ggagtgctgt gatggcgacg ttttctctcg atcgcaagct gtggggctgg ggatcggcgc
      361 tgtatcaggt gccggcgccg ctgctcgatc tggcgcgggc gtttttgtcg gcgcggcttt
      421 gctgtcacat tccccacgcc atgccggttc cgacgctgga ttcgatacgg atgccgaatc
      481 cgcggcgggc gataccgcat gatctggccg cgcttgtgac caccgatcgc tatcaccgcg
      541 cgacgcacac ctatggcaag gcgtatcgcg acgtggtgcg ggcgctcgac ggggattttt
      601 cgccggcccc ggactttgtc gcctatccgc aaaacgaagc cgacatcgaa cgcatcttgc
      661 ggattgcccg gcgcgagcac tgggcggtga ttccctatgg cggcggatcg agcgtggtcg
      721 gcggaatcga acccgatgca gcgacgcgga atcgcttttc agcggtgctg tcgctcgatc
      781 tgtgtgcgct tgaccagctt ctgcaagtcg atcgggcgtc gcgctgtgcc cgcatccaag
      841 ccggcgcgta tggtccgtct atcgaacagc agctcaaacc gcacggctac accctgcgtc
      901 acttcccgca gagctttgaa ttttcgacgc tgggcggctg gattgcgacc cgtgctggcg
      961 gccattttgc gacgctatac acccacatcg acgatctggt cgaaagcctg cggatggtga
     1021 cgccgcaagg cacactggaa acgcggcggc tgcccggaaa cggcgccggc ccgcaacaag
     1081 agaggctgct ctgcggttcg gaaggtgcat ttggggtgat ttccgaggcc tggctgcggg
     1141 tgcaagagct gccgcaacat cgggccagct gcatcgtccg ttttcccagc tttgccttgg
     1201 gagcccaggc ttgtcgggcg ctgtcccaaa gtggtctgtt tcccgcgaac gcgcgactta
     1261 tcgaggctga cgaggcgctg tttatgggaa tcggcgatgg cacacagcac ttgctgttgc
     1321 tcgggtttga gtcggcgcag ttttcccagc aagacaagct acaagcagcg gtggcgctgt
     1381 gcagccaggc gggcggaacg gcgggccggg ccaagatccg cagcggcgat agtgaactgg
     1441 aagacgacac ggcggcgcag tggaagcagt cgtttgtgcg ggcgccgtac ctccgcgatg
     1501 agctgtgccg aatgggcctt ttgtgcgaga cgtttgaaac ctgcacgacg tggtcccatt
     1561 tcccgaagct cgacgaagcc gtgcgggccg cagcgcagac ggcgctggcc gcgacttgtg
     1621 gtacagggct tgtcacctgt cggtttacgc atctttatcc cgacggacca gcaccgtatt
     1681 acaccgtgat tgcccaaggt acgccgggtc agcaagtggc ccagtgggac gcgataaagg
     1741 ctgcggtgtc gacggcactt gtcgaaaacg gagcaacgat tacccaccac cacgccgtcg
     1801 gaaaagacca tcgctcgtat tatttacgtc agggcgatcc gctggccatt gcaatgctgc
     1861 gagcggccaa aacggcagtt gatccaacgg gcattatgaa tccggggacg ctgcttccga
     1921 cggactagtc gccctcccaa ccctttcacc cagactttca ccagagtttc catggaagcc
     1981 gcacctcccc acattcgcgc gatggccaga gcgtcgctcg cgcttgatga cgacaagctc
     2041 atggcggagt gcgacgtcga tttgttcgtc gccagcgggc ccggcggcca gcatcgcaac
     2101 aaaaccgaaa gcgctgtccg gctcacccac cgcccaagcg gcatcgtcgt caccgccacc
     2161 gaacggcgca gccagatcca aaaccgcatc gtggcgctcg cccggctgcg cgaaaagctc
     2221 gaaaagctga cgtttgtgcc gcggcctcgg cgtccgacca agccgacccg cagctcgcaa
     2281 gagcgccggt tgagcgaaaa aaagcgcgac agccaagtca aaaagcagcg ccgcagcgat
     2341 tacgactgac gcggcgcgcg ttttttggcg ggggaagcga agtgactact tcgacaggaa
     2401 cgcgaggtgg tgggcggggg ttactttgcc gtcgacgcgg agcttgccgt gctgatacag
     2461 gctgcgcacg tcggctttgc cggcaaaaag cgccgccagg tcttcatcgc tgatcgtcag
     2521 cgtcgtgacc gcaccatcga ctttgccatc ggtgactttg ccgcttggat cgacggcaaa
     2581 cgcagcgtcg ggcgatttga cgacaaactg caaaagacca ccgatgtccg cgacgagccc
     2641 tggctgtttc gcgagcttgt cacgcagcgc cgcgacaatc gccggagctt gtgcagtctg
     2701 ggtttgcatc gcttgcgctg gggctgccgg tgcgtctttt ttgccgccgc cgccaccgcc
     2761 gccgccgcca ccgccgccga ggcgcgccat cacgaccttt tcgacccgct ttttgtccat
     2821 cttcatcagg aactcaagct tttggctggc catgacgttg ccgccgatct tgagcttgcc
     2881 gttcataaac agcttctgcg ggttggcttt gccgagcacc atgtcgagga agtcgctgtt
     2941 cgacaggctc agcgtcacat cggcatcctt gacaccttcg gcgagcgcgc ccggcgcgtt
     3001 cttcaggtcg atgtgatacg ccgaatcggg gccgctcagc ttgaacagga agctggtgcc
     3061 aatctgcttg gcgaggtcgg ggctgtcggc gatgtactct ttgatcgcga cgaagatgtc
     3121 cgtgctggtc aggaccgttg ccggatccac cgcagacggg gcttgggccg gggccgatcc
     3181 gccctttttg cccagggccg cttcgatttc gctgcggtcg atcttcttga ggaacatcag
     3241 cttctgactg gccatgatgt tgccgccaat cttgagcttg ccgccgaagt acagcttgtt
     3301 cgggtcggct ttccccgtcg tcatgtcgag gaagtcgctg tccgaaagtt cgagcgtgca
     3361 gtcaggctgc ggctcggtcc cggcgctgac agcccctttg ccattcttta ggtcgatggt
     3421 ccacacgctg tcggggccct tcaggcggaa ctgaaacacc gtaccgacgg aacccacgag
     3481 gtccggattt ttgccgatgt agccaccgat cgcggaaaac acgtcgccgc tcgtcagcac
     3541 tgcgggggct gcctgggctt gggccgcttg ggccggggcg ccgcctttgg ctttgggctg
     3601 cgggatgtct ttgtaaagct cgatcgccgc gtttgagatc acgactttgt ttcgttcaac
     3661 gacgctgacg cgggcgatga cgcgggtgtc ggactctttc cacagctcga tcttgagcgt
//
LOCUS       DAORGN010000317         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_14056_length_3710_cov_9.334337, whole genome shotgun sequence.
ACCESSION   DAORGN010000317 DAORGN010000000
VERSION     DAORGN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_3710_cov_9.334337"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /gene="thrS"
                     /locus_tag="PKL17_21020"
     CDS             <1..1208
                     /gene="thrS"
                     /locus_tag="PKL17_21020"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNK47278.1"
                     /translation="DLFTFSDLVGPGLPLFTPRGTLVRRLLEDFVQSLQEPLGYRRVQ
                     IPHITKNDLYKTSGHWEKFHEDLFHVRGKSGEEFCIKPMNCPHHTQIFACRQRSYRDL
                     PLRFSEVTSVYRDELPGALQGLSRVRMITQDDAHVFCTPEQVQDEALRIYHIIDSFYR
                     AFGMKLSIRLSLWDAEHPEKYLGSKEMWDSAQDQLRAVLRMRNATWVEEAGEAAFYGP
                     KIDFTAKDALDRAWQLATIQLDFNLPNRFDLKYIGEDGQPKRPAMLHRAVLGSVERFM
                     SVLIEHYAGAFPTWLSPLQAVVVPIADRHNEYAQQVVEKLSQVPLKNGLGTGIRAEVD
                     DSRESMQKKIRNAQLQKIPYMLVVGDREQEQGAAAVRLRSGVDLKAMPLSAIIDRLTT
                     EIETRTDLP"
     gene            1341..3671
                     /locus_tag="PKL17_21025"
     CDS             1341..3671
                     /locus_tag="PKL17_21025"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNK47279.1"
                     /translation="MLFLRLAPYELSLLVTLACLMLVWQRRRFPGARMFLAALACELA
                     YTIGYLGELLATTVPGKTFWDNVQWLPCLAGMLFLALFAREYAQKKPLPAWLLLQLGC
                     VPVAVATLAYSNDWHHYVGSGHRIIPGWPVPALTYEFGPVAWVAMPYLIGLALQSVTT
                     LFFFMLKRRGVFRRQALGILIGFLVPMVAYLATLFGLNVYGYRDVTPQALAFSNVFIL
                     WSLLRYRALQLLPFAREQVFDQIQGGVLVLDTSRQVVDANAAMASLLRIPLPKLLGES
                     ATVALSRWPTVLAAVERNVPTRSDLRDPDSAQERWLQVDIQAVSEGGQAGLLLLTRDI
                     TELKLATQEAMRSREAAIFASTAKSRFVATLSHEIRTPLAGVMGLSRLLGETKLDVHQ
                     RDLLQKLDSATRHMLELLNHTLDFSKLEAGRLELSPIHFDLGALLTSIAGIFFPTATD
                     KGLRFSLVVDPTVPQGLYGDGLRLKQILANLIGNAVKFTHRGEIGLHVSVREQQSADV
                     ELLFVVTDTGIGLGQEQVQALFRPFSQADATISHRFGGTGLGLSISHQLAQMFGGSLT
                     CESTKGVGSTFRLTVRLRISDRPVETKPAMPSENTSMASAPSAPLSTSASEPVTLSSS
                     DSSGVASERPKVLLAEDNQVNQLITQKMLRRIGYDCDIANNGGEAVQKLNSHPFDYYA
                     AVLMDLQMPDVDGRTAVIQLRADPRYDALPIIALTGESVREVILDVLSCGMDDVLHKP
                     VDLKTLGDVLKRLIREKRSGRDARSRDSASDASNPR"
BASE COUNT          684 a         1097 c         1137 g          792 t
ORIGIN      
        1 tggatctgtt cacgttttcg gatctcgtcg gtccgggtct gccgctgttt acgccccgcg
       61 gaacccttgt gcgtcggctg ctcgaagact tcgtccagtc gctgcaagag ccgctgggtt
      121 accgtcgcgt acagattccg cacatcacca aaaacgacct gtacaagacc tcaggtcact
      181 gggaaaagtt ccacgaggat ctgttccatg tgcgcggcaa gagcggcgaa gagttttgca
      241 tcaagccgat gaactgcccg caccacacgc agatatttgc gtgtcgtcag cgctcgtatc
      301 gcgatctgcc gctgcgcttt tccgaagtca ccagcgtcta tcgcgacgag ctgccgggcg
      361 cactgcaagg cctgtcgcgg gtgcggatga ttacccagga cgacgcgcac gttttctgta
      421 cgcccgaaca ggtgcaggac gaagcgctgc gcatttatca catcatcgac tcgttctatc
      481 gcgcctttgg catgaagctg tcgatccggc tgtcgctgtg ggacgccgag catccggaaa
      541 agtaccttgg cagcaaagag atgtgggaca gcgcgcaaga tcagctgcga gcggttctgc
      601 gcatgcggaa cgcgacctgg gtggaagaag ccggcgaggc tgcgttctac gggccgaaaa
      661 tcgacttcac ggccaaagat gcgctcgatc gggcgtggca gcttgcgacg atccagctcg
      721 acttcaacct gccgaatcgc ttcgacctca agtacatcgg ggaagatggg cagcccaagc
      781 gtccggcgat gctgcatcgc gccgtgctcg ggtcggtcga gcggtttatg tccgtgctca
      841 tcgaacacta cgcgggtgca ttcccgactt ggctgtcgcc gctgcaagcc gtggtggtgc
      901 cgattgccga taggcacaac gaatacgccc agcaggtggt cgaaaagctg tcgcaggtgc
      961 cgctgaaaaa cggtctgggc actggcattc gggccgaagt cgatgactcg cgcgagtcga
     1021 tgcaaaagaa gattcgcaac gcccagctgc aaaaaattcc gtacatgctg gtcgtcggtg
     1081 atcgcgagca agagcagggc gcagcggcgg ttcggttgcg ttcgggcgtc gacctcaaag
     1141 cgatgccgct ttcggccatc atcgatcgtc ttaccaccga gatcgaaacc cgcaccgacc
     1201 tgccgtagtt tccgcaccct acctgccgtt tccctgcaag ccgtcgccgg cacatttttt
     1261 cgtcagcaaa tctgcgaggg tatcggcacc acgacagcaa ttggtacgat atctatagag
     1321 gcgcaaaagc ctcttcccgg atgctgtttc ttcggctggc tccatatgag ctgtcgctgc
     1381 tggtcacgct tgcttgcctg atgttggttt ggcaacggcg gcgttttccg ggtgcgcgca
     1441 tgtttctcgc cgcactggcc tgcgagctgg cttacaccat cggctatctc ggagagctgc
     1501 tcgcgacgac ggttcccggc aagacgttct gggacaatgt gcagtggctg ccgtgcctcg
     1561 cggggatgct gtttctggca ctgttcgcgc gcgagtacgc ccagaaaaaa ccgctgcccg
     1621 cgtggctttt gctccagctt ggctgtgtgc cggtcgccgt cgccacgctc gcttatagca
     1681 acgactggca ccactacgtt ggcagtggac atcgcatcat ccccggctgg cccgtcccgg
     1741 cgctcaccta cgagtttggc cccgtcgcgt gggtggcgat gccctatctt atcgggctgg
     1801 cgctccagtc ggtcacaacg ctgttctttt tcatgctgaa gcggcgtggg gtgtttcgtc
     1861 ggcaagcgct ggggattctc atcggctttt tggttccgat ggtggcgtac ttggccacgc
     1921 tgtttggtct gaatgtctat gggtatcgag atgtgacgcc gcaggccttg gcgtttagca
     1981 acgtgtttat cctttggtcg ctcttgcggt atcgggcgct acagcttctg ccgtttgcgc
     2041 gcgagcaggt gttcgatcag attcagggcg gcgtcctcgt ccttgatacg tcgcgacagg
     2101 tggtggatgc caatgcagcg atggcgtcgc ttttgcggat tcctctaccg aaactgctcg
     2161 gtgaatcagc gacggtggca ctgtcgcgct ggccgacggt gctggcggcg gtcgagcgaa
     2221 acgtaccgac gcggagcgac ctaagagatc ccgattcggc gcaagaacgc tggctgcaag
     2281 tcgacattca agcagtgagc gagggcggcc aagctgggct tttgctttta acccgcgaca
     2341 tcaccgagct gaagctggcg acgcaagaag cgatgcgatc gcgggaagct gccatttttg
     2401 ccagcacggc caagagtcgt tttgtcgcga cgctgagcca tgaaattcgg acgccgctgg
     2461 ccggtgtgat ggggctgtcg cgtctgctcg gcgagaccaa gcttgatgtc catcagcgcg
     2521 acttgctcca gaagctcgac agtgcgacgc ggcatatgct ggagctgcta aaccacacgc
     2581 tcgacttttc aaagctggaa gctggccggc tcgagctttc gccgatccat tttgatctgg
     2641 gcgcgctgct gacgtcgatc gctggtattt tttttccgac ggctaccgac aaagggctgc
     2701 gcttttcgct tgtcgtcgat cccacggttc cgcaaggcct gtacggcgac ggacttcggc
     2761 tgaagcaaat cctggccaat ctgatcggca acgccgtcaa gttcactcac cgaggcgaga
     2821 ttggtctgca cgtctcggtc cgcgagcagc aatccgccga tgtcgagctg ttgttcgtcg
     2881 tcaccgacac cggaatcggg ctcggccaag agcaagtgca ggcgctgttt cgaccgtttt
     2941 cgcaagctga cgcgacgatt tcgcatcgtt tcggcgggac ggggcttgga ctttcgataa
     3001 gtcatcagct tgcgcagatg ttcggcggca gcctgacctg cgagagcaca aaaggcgtcg
     3061 gcagcacgtt ccgactgact gtacggctgc gcatcagtga ccgtcctgtc gaaaccaagc
     3121 cggcgatgcc cagcgaaaat acgtccatgg cgagcgctcc gtctgcgccg ctttccacca
     3181 gtgcgagtga gcccgtgact ctcagttcgt cagattcgag tggtgtggct tcagaacggc
     3241 ccaaagtgct gcttgccgag gacaaccagg tcaaccagct gatcacgcag aagatgctgc
     3301 gacggatcgg ctacgactgc gacatcgcaa acaatggcgg cgaggccgtg caaaagctaa
     3361 acagccatcc gtttgattac tacgcggcgg tgctgatgga tttgcagatg cccgatgtcg
     3421 atggcaggac ggcggtaatt cagctccgcg cggatcctcg ctatgacgcg ctgccgatca
     3481 ttgcgctgac cggggaatcg gtgcgcgagg tcattctcga cgtgctgtcg tgtgggatgg
     3541 acgacgtgtt gcacaagccc gtcgatttga agaccttggg cgacgtgcta aagcgtctga
     3601 ttcgcgaaaa gcgctctggc cgcgacgctc ggtcccgcga ttccgcaagc gacgcttcca
     3661 atccccgcta gccgttccgc aatcccccga cgccgagaaa ccaccccccc
//
LOCUS       DAORGN010000318         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_14319_length_3670_cov_6.206916, whole genome shotgun sequence.
ACCESSION   DAORGN010000318 DAORGN010000000
VERSION     DAORGN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14319_length_3670_cov_6.206916"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="PKL17_21030"
     CDS             complement(<1..1954)
                     /locus_tag="PKL17_21030"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNK47280.1"
                     /translation="MSGGTDSKPQVPSSETQPTSATSGPALVGPGLSGQSSPSGVSNP
                     SGFSNPSGVSNPSGFSNPSGFSNPSGVSNPSGFSNPSGFSNPSGVSNPEAGPLPIDPM
                     LDGPTPPSAESPPPRLDPAVLAQRETLPLARPPADPQPQRRIEETVEVPSFAPDAATE
                     VSSWNSPLWQRRSSFFVQVFFWLFGMALCMSLGSGAIFYNQQYRFLEEDQRQRSRLLL
                     SSLAYQCLLGAYAMDRSILSQPVQQMAQLDDVEFVAVYDAQGVELLKTTTRTGKPMLD
                     PTPAKLRSYVADVLIRTAKKKPAKPISRDALDPGLKLEPLEERHETYVDLYAPMVLPD
                     RGGPGLGAFINPLDLSVRDRVVGIVRIGLSIEPAQERLRQVLFFSVGLGVFLMMLGGG
                     AALFIAGRLSGPIMALARGADEIRSGNLHPRISVRRSDELGLLADSFVRMAERLRESM
                     QALSQLNRNLESEIKRRTKQLRLAYAFTSVLNSPIDRSGDAMDAADLTVMLDQALAAL
                     VEATGTRGGGVFLLEDGATGEPEFLVRAAYGVSTHKLGQPPSVREFSQPATATAAPAL
                     SDAVKVQGRRILVPLTFRNQPLGLLVLVMTSDETPDGSLLEFVRQAAAQLSIAVSNAR
                     AYARVGRLAHELVLNNEALAAQRDQMQ"
     gene            complement(1951..2913)
                     /locus_tag="PKL17_21035"
     CDS             complement(1951..2913)
                     /locus_tag="PKL17_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNK47281.1"
                     /translation="MAISAAMLVLGALTSLGLGAALPDGPVDGNGGGVVQRPSAPVVV
                     VKSANVKAFQDVAESFRDRCRVPVFFINLKHESADEAARVREAVASARLLVAVGQPAA
                     EALQGLRTRIVYAMVPSPPVGIVGTNSSVSPRDALAALRMLKPDIRHVGVIYSRQGLT
                     KMVAARAAARALGVELHDQLIESSPDAIRAIHSLVWGRTDETSHRTPKVDALWVGPDP
                     LVVDMPLLQYLLTLQLTARVPVLAGTRQMVSHGVLMSIDWSPDAVGENLALQVNQILD
                     DPNHYELLRDHPVTPPDIIINGPAARRLGVRLEPFIELGWKVHE"
     gene            3155..>3670
                     /locus_tag="PKL17_21040"
     CDS             3155..>3670
                     /locus_tag="PKL17_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47282.1"
                     /translation="MSEQKPSWLWSAFRIVTRARWLIVAIFALLLGPAVYFALKVPQD
                     NSIDRLIVRDDPDYLASRAFAKVFGDSEYIILLAEAPDPFAGPVVKRIDEMENRLRSI
                     PRVSVNSALTIFRRAKAGFSGTDAELAAFRKFATGTKLFSRQGLVGDHFISLALVLSI
                     KNTDERQETLGN"
BASE COUNT          754 a         1220 c         1031 g          665 t
ORIGIN      
        1 gctgcatctg gtcgcgctgc gccgccagcg cttcgttgtt gagcaccagc tcgtgcgcaa
       61 gacgtccaac gcgggcatac gcgcgggcat tgcttaccgc gatcgaaagc tgcgcggcgg
      121 cttggcggac aaactcaagc agcgatccgt caggcgtttc gtcgctcgtc atcaccagca
      181 cgagcaggcc gagcggctgg ttgcggaacg tgagcggcac caaaatccgc cgcccttgca
      241 ctttgaccgc atcgctcagc gccggagccg ccgttgctgt agctggttgg gaaaactcgc
      301 gcaccgatgg tggctgtccc agtttgtgcg tcgagacccc gtaagctgcc cgaacaagaa
      361 actccggttc ccccgttgcg ccgtcttcga gcaaaaacac cccgccaccg cgtgttccgg
      421 tcgcttcgac gagcgctgcc agcgcttgat ccagcatgac ggtcagatcc gccgcatcca
      481 tcgcgtcgcc gctgcgatcg atcggggagt ttaggaccga cgtaaacgca taggccaggc
      541 gcagctgctt ggtccgtctc ttgatctcgc tttccaagtt gcgattgagc tgcgacagcg
      601 cctgcatgct ctcgcgcagc cgttcggcca tgcggacaaa cgaatcggcc aaaagaccca
      661 gctcgtcgct gcgccggaca ctaatgcgag gatgcagatt gccgctgcgg atctcgtcgg
      721 ccccgcgcgc cagtgccatg atcgggccgc tcagccttcc ggcgataaac agcgccgcgc
      781 cgccccccag catcatcaaa aaaacgccga gaccgacgct gaagaacagc acctgccgca
      841 gacgttcctg cgccggttct atcgacaggc caatccgcac aatcccgacg acccgatcgc
      901 gcaccgacag atcgagcgga ttgataaacg ccccaagccc aggtccaccg cgatccggca
      961 gcaccatcgg cgcatacagg tcgacatacg tttcgtgccg ttcctcgagc ggttctagct
     1021 tgagtcccgg gtcaagcgcg tcgcggctga tcggcttggc tggctttttc ttggccgtcc
     1081 gaatcagcac gtcagcgaca tacgagcgca gcttcgccgg ggtcggatcg agcatcggct
     1141 tgcccgttcg cgtcgtcgtt ttgagcagct ccaccccttg cgcatcgtag acggcgacaa
     1201 actccacatc gtcgagctgc gccatctgct ggaccggctg cgacaaaatg gaccgatcca
     1261 tcgcatacgc cccgagaaga cactgatacg cgagcgagct tagcaagagc cgcgatcgct
     1321 gacgctggtc ttcttcgaga aagcggtact gctgattgta aaaaatcgcc cccgacccca
     1381 gcgacataca cagcgccatc ccaaacagcc agaaaaagac ctgaacaaag aagctggacc
     1441 gtcgctgcca aagaggagaa ttccagcttg atacctcggt ggcggcatcg ggggcaaacg
     1501 acggcacttc gacggtttct tcgatccggc gctgcggctg cggatcggca ggcggcctcg
     1561 ccagtggcag agtctcgcgc tgcgccagca ccgccggatc aagccgcggc ggcgggctct
     1621 ctgccgacgg aggagtcgga ccatcgagca tcggatcgat cggcagcgga cccgcttctg
     1681 gattcgacac gcccgaagga tttgaaaaac ccgacggatt tgaaaaacca gatggattcg
     1741 acactcctga cggatttgaa aagccagacg gatttgaaaa acccgacgga ttcgacactc
     1801 ctgacggatt tgaaaaaccc gacggattcg acactcctga cgggctcgat tgtcccgata
     1861 gaccaggccc aacaagtgcc ggccccgatg ttgccgacgt gggctgcgtc tcgcttgagg
     1921 gaacctgtgg cttgctatct gtgccgccac tcattcgtgc accttccagc ccagctcgat
     1981 aaacggctcc agtctgaccc ccaaacgccg agccgccggt ccgttgatga tgatgtcagg
     2041 tggcgtcact ggatgatcac gcagtagctc gtaatggttc gggtcatcga gtatctggtt
     2101 gacttgtagc gccaggtttt cccccacagc gtcgggggac cagtcgatgc tcatcagcac
     2161 gccgtgtgac accatctgcc gcgttcccgc cagcaccggc acccgcgccg tcagctgcaa
     2221 cgtcagcaaa tactgaagca gcggcatgtc gacgaccagc ggatcgggcc ccacccacag
     2281 cgcatcgact ttcggcgtcc tatggctggt ttcgtcggtg cgaccccaca ccaagctgtg
     2341 aatcgcgcga atcgcgtcgg ggctcgattc gatcagctga tcgtgcagtt cgactcccag
     2401 cgctcgcgcc gcggcccgcg ccgccaccat cttcgtcagc ccctgccgcg agtagatcac
     2461 accgacgtga cgaatgtccg gctttagcat ccgcagcgcc gcaagcgcat cgcgcgggct
     2521 gacactgctg tttgtaccaa caatgccgac gggtggagac ggaaccatcg cataaacaat
     2581 ccgcgtgcgc agcccttgca aagcttccgc cgcgggctgc cccaccgcca ccaacagccg
     2641 tgcactcgca acggcttcac gtacccgcgc tgcttcgtcg gcagattcat gctttaggtt
     2701 gatgaaaaaa accggcaccc gacagcgatc gcgaaacgac tctgcgacat cttgaaaggc
     2761 tttgacgttg gcacttttga cgaccaccac cggcgcgctc ggcctttgca caacgccccc
     2821 accatttccg tcgacgggac catctggcag ggcagcccca agtccaagcg aggtgagcgc
     2881 gccaagcaca agcattgcag ccgaaatcgc catgacccag caattgtgag ggcaaatgcc
     2941 cagacgtgcc agcgcaaccc aggagaaaca agcctgcgcg cctgtcgccc ccagccacag
     3001 cgaccgaaag gctcacatgt cccgtcgaat gtcgccccgt ttgcaaaagc attttctcgg
     3061 cacgccgcat cggctagcat ggcccgaacc catccctctg cgcaggtggc gtggcatcta
     3121 aaaagccaat ctggccgtcg cagaaagtaa ctccatgagc gaacaaaaac ccagctggct
     3181 gtggtcggct tttcgaatcg tcacgcgagc acgctggctc atcgtcgcga tcttcgcgct
     3241 tttgcttggg cctgcggtct atttcgcact caaagtcccg caagacaact cgatcgatcg
     3301 gctgatcgtc cgcgacgatc ccgactatct ggcgtcgcgc gcgtttgcca aagtcttcgg
     3361 cgattccgaa tacatcatcc tgcttgccga agcgcccgac ccgtttgccg gtccggtggt
     3421 caagcgcatc gacgaaatgg aaaatcgact gcgcagcatt ccccgggtgt cggtgaactc
     3481 ggcgctgacg attttccgtc gggccaaagc cggtttttcc ggcaccgacg ccgagcttgc
     3541 cgcattccgc aagtttgcca ccggcaccaa gctattcagt cgacaagggc tcgtcggtga
     3601 ccactttatt tcgctcgcat tggtgctttc gatcaaaaac accgacgagc gacaggaaac
     3661 gctagggaac
//
LOCUS       DAORGN010000319         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_14769_length_3608_cov_8.382494, whole genome shotgun sequence.
ACCESSION   DAORGN010000319 DAORGN010000000
VERSION     DAORGN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14769_length_3608_cov_8.382494"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="PKL17_21045"
     CDS             <1..1556
                     /locus_tag="PKL17_21045"
                     /inference="COORDINATES: protein motif:HMM:NF015836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47283.1"
                     /translation="SGVGSGRGAQGLGNRGEDSADARLWQQTRRLLLVGLAFYLVAAL
                     FGEGFIHPDEHFQILEFASARLRLSPLADLPWEFQERIRPSLQPVLAMGALWLFRSVG
                     IVSPFVVATALRTCIAVLAWVVSSRAVLFLSRRLVRKQSRALLAFGVMGLWFMPLLSA
                     RFSSENLSAILLLSSLLYLGPAADERPCPVSARAFFVAGVLWGLSFFARFQIAFALVG
                     ISLWIGFVARPGLFRIALLALGCLSVGLACVALDSWFYGQLTFSPLRYFVANLIEHRA
                     AEWGVEPWWWYIPQAALWTFPLIGLPLVVFALRGVAKSRQDALLFWVVPFLLGHAAIG
                     HKELRFLFPLAIPVVYLAVRGVDSMLQGKSRPVLPRWAVPVAVLGNLILFGLRMLIPL
                     SSEVRLAHAVWDVADLRGPTTVVTVKTPLDEWFGLPMHFYRHPAVHSEVAADLAVLAR
                     RLSASTAPTTYVVLSTWDAVPLGPGTSAQVLYCYPPAWTEPFLPGPVKRLITPLRLFE
                     VRGAARAME"
     gene            complement(1617..3221)
                     /locus_tag="PKL17_21050"
     CDS             complement(1617..3221)
                     /locus_tag="PKL17_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNK47284.1"
                     /translation="MEPESLLSKEIAREVDRRRTFAIISHPDAGKTTLTEKLLLFGGA
                     IQMAGAVKARKAARHARSDFLAIERERGISVSSSVMKFNYQDIEVNLLDTPGHQDFSE
                     DTYRVLTAVDSALMVIDSAKGVETQTRKLMEVCRLRDTPIITFINKLDRDGREPFDLL
                     SDIENSLKIQCAPMTWPIGMGKQFRGTFSLFRNELRLFSGTGSERVQDAVVYHGAEDP
                     ALDDVLGTQVGHLRSDLELLQGAGTPFDKQAYLAGKMTPVFFGSAVNNFGIAELLDAL
                     LQFAPPPRPRETTTRMVSPYETGFSGVVFKIQANMDPQHRDRIAFLRICSGKFNRGMK
                     VRHHRIGKDVQIQNATIFMAQDRSGVEEAYPGDVIGIHNHGTIKIGDTMSDKEPLKFV
                     GIPNFAPEHFRRVRLLNPLKSKQLEKGLLHLAEEGAVQLFRPLRNNDYILGAVGVLQF
                     EVTIARLGDEYGVEATYEPVEYGAARWVRSEDKKKLEEFRSKAASNLAVDIDGNLTYL
                     AENDWRLNHTIDKWPGIVFDRTREHV"
     gene            3304..>3608
                     /locus_tag="PKL17_21055"
     CDS             3304..>3608
                     /locus_tag="PKL17_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNK47285.1"
                     /translation="MSQDTPEQFPSFESFYPFYLSQHENQTCRRLHFIGSSLAALWGL
                     RAMWNGSVAQAACGLVIGYGFAWVGHFFFEKNRPATFKFPLYSFMGDWVMWKDIATG"
BASE COUNT          541 a         1020 c         1183 g          864 t
ORIGIN      
        1 cgtccggcgt tggctctgga cgcggcgcgc aaggcttggg caaccggggc gaggattcgg
       61 cggacgcacg gctttggcag caaacaagac ggcttctcct cgtcgggctt gctttctatc
      121 tggtggcggc gctgtttggt gaaggcttca tccatccgga tgagcatttc cagatattgg
      181 agtttgcaag cgcgcggctc agactgtcac cgctcgcgga ccttccgtgg gaatttcagg
      241 agcggattcg tccgagcttg cagccggtgt tggcgatggg tgcgctttgg ctgttccgca
      301 gcgttgggat tgtcagtccg tttgtggtgg cgacagcact gcgcacctgc atcgcggtgc
      361 tggcttgggt cgtaagcagt cgggctgtac ttttcctgtc gcgacgactg gtccgcaagc
      421 aatcacgggc gctgctggct ttcggggtga tggggctgtg gtttatgccg cttttgtcgg
      481 cgcgcttttc atcggaaaac ctctcggcga ttttgttgct cagcagcctg ctgtatctgg
      541 gccccgccgc ggatgaaaga ccttgtcctg tttcggcgcg cgcgtttttt gtcgctggcg
      601 ttctgtgggg tctgtccttt ttcgcccgct tccagatcgc gtttgctctt gtcggaatca
      661 gtctgtggat cggctttgtg gcgcggcctg gtctgtttcg tatcgcgctt ttggcgctgg
      721 gctgtctgtc cgtcggtttg gcctgtgtcg cgctggatag ctggttttac ggacagctta
      781 ctttttcacc gctgcgctac tttgttgcca acctgatcga gcatcgcgcg gcggagtggg
      841 gcgtcgagcc gtggtggtgg tacattcctc aagcggcgct gtggacgttt ccgctcatcg
      901 gtttgccgct ggtggtgttc gcgctgcgtg gggtggcaaa gagtcgccag gatgcgctgt
      961 tgttttgggt ggtgcctttc ctgcttggac acgctgccat cggccacaag gagctgcgct
     1021 ttctgttccc gctcgccatt ccggtcgtgt atctggcggt gcggggcgtc gattcgatgc
     1081 tgcagggcaa aagccggccc gttctgccgc gctgggccgt cccggttgcg gtgcttggca
     1141 acctgattct gttcgggctg cggatgctga ttcccctgtc atcggaggtg cggctggctc
     1201 acgcggtctg ggacgtggcg gacttgcggg gcccaacgac ggtagtgacc gttaagacgc
     1261 cgcttgatga gtggtttggt ctgccgatgc acttctaccg tcatcccgcc gtgcacagcg
     1321 aagtcgcagc ggacttggct gttctggccc gtcggctttc ggcgtcgacc gcaccgacca
     1381 cgtatgtcgt gttgtcaacg tgggatgccg tccccttagg gcctgggact tccgcacagg
     1441 tgctgtactg ctatccgccc gcgtggacgg aacctttttt gcctggacct gtgaagcggc
     1501 tgatcacacc gctgcgtctt ttcgaagtcc gcggagcggc gcgggcgatg gagtgaccga
     1561 gcagagcttg cgctttgccc catggcgtgt tcgccagcgc ggcgttccgg tggggactag
     1621 acgtgttcgc gggtgcggtc aaacacgatg ccgggccact tgtcgatggt gtggtttaga
     1681 cgccagtcgt tttcggccag gtaggtcagg tttccgtcga tgtcgaccgc gaggttcgag
     1741 gcggctttgc tgcggaactc ttcgagcttc tttttgtctt cgctgcggac ccagcgcgcc
     1801 gcaccgtatt cgacgggttc gtaggtggct tcgacgccgt attcatcgcc aagccgcgca
     1861 atcgtgactt caaactggag cacaccaacg gctcccaaga tgtaatcgtt gttgcgcagc
     1921 ggacggaaaa gctgcaccgc gccttcttcc gccaggtgaa ggagtccctt ttcgagctgc
     1981 ttgctcttga gcggattgag caggcgcacg cggcggaagt gttcgggcgc gaagttggga
     2041 atgccaacga acttgagcgg ctctttgtcg ctcatggtgt cgccgatttt gatggtgcca
     2101 tggttgtgaa tgccgatgac gtcgccggga taggcttctt cgacaccgga tcgatcctgc
     2161 gccatgaaga tggtcgcatt ctggatctgg acatccttgc caatgcggtg gtggcggacc
     2221 ttcatgccgc ggttgaactt gcccgaacag atgcgcagaa acgcgatgcg gtcgcggtgc
     2281 tgcgggtcca tgttggcctg gatcttaaac accacgccgg aaaagcccgt ttcatacggg
     2341 gacaccatgc gggtcgtggt ctcacgcggg cgcggtggcg gtgcaaactg gagcagtgca
     2401 tccagaagtt cagcgatgcc gaagttgttg accgcactgc cgaaaaagac gggcgtcatc
     2461 ttgccggcca gatacgcctg cttgtcaaac ggcgtcccag cgccttgcaa tagttccaga
     2521 tccgatcgca ggtgtccgac ctgggttccg agcacgtcat cgagcgcggg atcttccgcg
     2581 ccgtgataca ccaccgcgtc ctgcacccgt tccgatccgg tgcccgaaaa cagccgcagc
     2641 tcgttgcgaa acaagctgaa ggtgccgcgg aactgcttgc ccatgccaat cggccaggtc
     2701 atcggcgcgc actggatctt caggctgttt tcgatgtccg acagaaggtc gaatggctcg
     2761 cgtccgtcgc ggtcgagctt gttgataaac gtgatgatcg gtgtgtcgcg caggcggcag
     2821 acctccatca gcttgcgcgt ctgggtttcg acgcccttgg cgctgtcgat gaccatcagg
     2881 gctgaatcga ccgcggtgag cacgcgatag gtgtcttcgg aaaagtcctg gtggcccggc
     2941 gtgtcgagga ggttgacctc gatgtcctgg tagttgaact tcatcaccga ggagctgacc
     3001 gagatgccgc gctctctttc aatcgccagg aagtcactgc gggcgtgccg ggcggctttg
     3061 cgggctttga cggcgccggc catctgaatc gcgccaccga acagcagcag cttttctgtg
     3121 agcgtcgttt tgccggcgtc ggggtggctg atgatggcga aggtgcggcg gcgatcgact
     3181 tcgcgggcga tctctttgga gagcaggctt tctggttcca cggcagcgct tcgttaccta
     3241 aacctttcgc tgccgtccat ggaaaacgac cacgggcgcg gcccactcgc tatagtctct
     3301 tgcatgagtc aggacacgcc cgagcagttc ccgagcttcg aatcgtttta tccgttctat
     3361 ctatcgcagc atgaaaatca gacctgccgt cggcttcact tcatcggcag ctcgctggcc
     3421 gcgctgtggg gcctgcgcgc gatgtggaac gggagtgtgg cgcaggctgc ctgcggactt
     3481 gtcattggct atggctttgc ctgggtcggg cactttttct ttgaaaagaa ccgcccggcg
     3541 acgttcaagt tcccgctcta cagcttcatg ggcgactggg tgatgtggaa agacatcgcc
     3601 accggcaa
//
LOCUS       DAORGN010000320         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_14891_length_3588_cov_5.425134, whole genome shotgun sequence.
ACCESSION   DAORGN010000320 DAORGN010000000
VERSION     DAORGN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14891_length_3588_cov_5.425134"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1298)
                     /locus_tag="PKL17_21060"
     CDS             complement(3..1298)
                     /locus_tag="PKL17_21060"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNK47286.1"
                     /translation="MTAPQYEVIIVGGRPAGSSLALRLGRAGLRVLLIEKARLAEQSP
                     LSVPFLMNSAMQLLDELDLAEADYAGDAPRLRRFYLHFRDSFRTHLHIADVAGRDYVY
                     VVDRVRFDCALWHKLASQPTVDCRQQSQLVDLLRDTSGQVCGITYRDAGGQLRTAHAP
                     WVVGADGRYSLVARKAQARITLSHPEVATSALYAFWSGVRPYEPRDGEPHAVQIYSSC
                     DGFSTIAMPTTAGRVGIVLQGCADRFLPKTGHIEDYYLAGLRKMPAVWDRLGKAQRVS
                     PMFGCKRIGNLYRQAGGPGWLLCGDAYHQKDFIDAQGIYDALLESKLLAGCLIDWHSG
                     KLAAADVVSRYDREAFAATHPMFQATVDRLKRELFTTLPPLVIKTVLRTLMTSPAYMQ
                     RFAKLVSRQIEPKDFAPKTFLLTTLLKNLPHVLLGKEGG"
     gene            complement(1332..1766)
                     /locus_tag="PKL17_21065"
     CDS             complement(1332..1766)
                     /locus_tag="PKL17_21065"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNK47287.1"
                     /translation="MRELAPISGHRLFQDGPLLVYRQHENFTLPDAQASTAIYSDVIA
                     KEGYLLLLLDFRHGGSADGDVRRHLAHFGKENADRLCIAAVGGNFMLRTTLTLVLTAA
                     KVLGGRQPTVQFFGNEEDALAWLAASGANLSTLVHNRPPPNP"
     gene            complement(1821..3263)
                     /locus_tag="PKL17_21070"
     CDS             complement(1821..3263)
                     /locus_tag="PKL17_21070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNK47288.1"
                     /translation="MKLGIRLKLFFLSLSLIALSLGISYGYLRRTLDANLTTQIEQDL
                     TQRLRLVAHSAETARLQPSDPKRWQETARALAPLCAARVTLLRQDGMPLADSEVEDDG
                     LAGLPNHLGRPEIAQAQLRGYGQSTRQSATLKRRMMYVAMPFLQADQRPGVARLAMPL
                     TAVDQAMGQLHLALLVASVLGLGVAVLMSSAAAHLAARSLQKLTDIAHRMARGDLAMR
                     TGSAGQDELAELGRDLDSLADSLSKALAELRKLESLRREFVANASHELRTPIAALLSA
                     VETLRAGAAQDPKARETFLDIIYRQAARMHSLVSDLLDLSRLERQDLKLTLLALPLVD
                     VVKQQVALFLPRAEERKLRLVISPNLESCQVLADRQCIDQVLSNLLDNAIKYCTSGDT
                     ISVQWSVDAENRQVHIEVKDTGPGIAENHLPRLFERFYRIDTGRSRSLGGTGLGLSIV
                     KHAVEAMKGKVSVDSEVGKGSTFRFTLQAA"
     gene            complement(3267..>3588)
                     /locus_tag="PKL17_21075"
     CDS             complement(3267..>3588)
                     /locus_tag="PKL17_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNK47289.1"
                     /translation="ITIGALRIDRSAHRVFVGSEEKKLTALEFRLLWTLASQHGKVLS
                     RAQLLSTAWQDWNVEGAITTRTVDTHVKRLREKIGDEGELIETVRGVGYRFRTPFDGS
                     VQGS"
BASE COUNT          671 a         1127 c         1169 g          621 t
ORIGIN      
        1 ccctacccac cctctttccc cagaaggacg tgggggaggt tttttaggag ggtggtgagg
       61 aggaaggttt tgggagcgaa gtctttgggt tcgatttggc ggctgacgag ctttgcgaag
      121 cgctgcatgt aggcggggct tgtcatgagc gtgcgcagga cggtcttgat gacaaggggc
      181 ggcagggtcg taaacagctc gcgcttgagc ctgtcgacag tggcttggaa catcgggtgg
      241 gtggcggcaa aggcttcccg gtcgtagcgg gacaccacgt cggcagccgc gagcttgccg
      301 ctgtgccagt cgatgagaca gccggcgagc agcttggact ctagcagcgc gtcataaatg
      361 ccctgcgcgt cgatgaagtc cttttgatgg taggcatcgc cgcagagcag ccagccggga
      421 ccgcctgcct gccgatacag gttgccgatg cgcttacacc caaacatcgg cgacacgcgc
      481 tgggctttgc caagccgatc ccagaccgcc ggcatcttgc gcagcccggc caggtagtag
      541 tcttcgatgt ggccggtctt gggcaaaaag cggtcggcgc agccttgcag gacaatgccg
      601 acgcggcccg cggtggtcgg catggcgatg gtgctaaaac catcgcacga tgaataaatc
      661 tgcaccgcat ggggctcgcc gtcgcgcggc tcgtaggggc ggacgccaga ccaaaaggcg
      721 tagagggcgc ttgtcgcaac ctcgggatgc gacagggtga tgcgggcctg tgctttgcgg
      781 gcgaccaggc tgtaacgacc atcggcaccg accacccagg gggcgtgcgc ggtgcgcagc
      841 tgaccgccag catcgcggta ggtgatgccg cagacctggc cgcttgtgtc ccgcagcaga
      901 tcgacaagct gggactgttg ccggcagtcg acggtcggct gcgacgcgag cttgtgccac
      961 agcgcgcagt cgaagcggac ccgatcgacg acgtagacgt agtcgcgccc ggcgacatcg
     1021 gcaatgtgca ggtgggtgcg aaaggaatcg cggaagtgca ggtaaaagcg acgcaggcgc
     1081 ggcgcatccc cggcgtagtc ggcttcggcc agatccagct catcgagcag ctgcatggcc
     1141 gagttcatca aaaacggcac agacagcggc gactgctcgg caagccgcgc tttttcgatc
     1201 agcagaaccc gaagacccgc ccgtccgagc cgcagcgcca ggctgctgcc agctggacga
     1261 ccaccgacaa tgatgacctc gtactggggt gccgtcatgc cgctactatc gcgaaagaag
     1321 cggcagcaag gctatgggtt tggtggggga cggttgtgta ccagggtgga caggttggcg
     1381 ccagatgccg ccagccaggc cagcgcatcc tcttcgtttc cgaaaaactg caccgtgggc
     1441 tgccgaccgc ccagcacctt ggccgccgtc agcaccaggg tcagcgtcgt ccgcagcatg
     1501 aagttgccac cgactgcggc gatgcacagc cggtcggcgt tttctttgcc aaagtgcgcc
     1561 aggtgccgac ggacatcgcc atctgccgat ccgccgtggc gaaagtcgag cagcaagagc
     1621 agatagcctt ccttggcgat gacatcggaa tagatcgcgg tcgacgcctg ggcatcgggc
     1681 agcgtgaagt tttcgtgctg ccggtagacg agcagtggcc cgtcctgaaa cagtcgatgt
     1741 cccgaaatcg gagccagctc tctcatcccc cgctccccgg cgtgcacatg gaccaagatg
     1801 ttacgcgatc cgggaacagg ttatgcggcc tggagcgtaa agcggaacgt cgatcctttg
     1861 ccgacctcgc tgtcgacgga taccttgcct ttcatcgctt cgaccgcgtg cttcacgatg
     1921 gacagaccca gtccggtccc gcccagcgat cgagatcgcc cggtgtcgat gcgataaaag
     1981 cgctcaaaca gcctgggcag gtggttttcg gcgatgccgg ggccggtgtc tttgacttcg
     2041 atgtggacct ggcggttttc ggcgtcgacg gaccactgca cggaaatcgt gtcgccgctt
     2101 gtgcagtatt tgatggcgtt gtcgagcagg ttcgacagaa cctggtcgat gcactgccga
     2161 tcggccagca cctgacagct ttctaggttg ggtgaaatga cgagccggag cttgcgctcc
     2221 tcagcacgcg gcaaaaacag cgccacttgc tgcttcacca cgtcgacaag tggcagcgcc
     2281 aaaagcgtca gcttgaggtc ctgccgttca agccgcgaca gatcgagcag atcactgacc
     2341 agcgaatgca tgcgcgccgc ttgccggtaa atgatgtcga ggaacgtctc gcgcgccttg
     2401 ggatcttggg ctgcgccggc ccgcagcgtc tcgaccgccg acaagagcgc ggcaatcggg
     2461 gtccgcagct catgcgaggc attggcgaca aactcgcgcc gcagcgactc cagcttgcgc
     2521 agctccgcca gcgctttcga cagcgagtcg gccagcgagt ccagatcgcg ccccagctcc
     2581 gccagctcat cttggcccgc cgatcccgtg cgcatcgcca agtcgccgcg cgccatccgg
     2641 tgggcaatgt cggtcagctt ttgcagactg cgcgccgcca ggtgggccgc ggcgctcgac
     2701 ataaggaccg cgaccccaag ccccagcacc gacgcgacca aaagcgccaa gtgaagctgc
     2761 cccatcgcct gatcgaccgc cgtcagcggc atcgccagcc gggccacgcc gggccgctgg
     2821 tcggcctgca aaaacggcat cgcgacgtac atcatccgtc gcttgagcgt cgcgctctgc
     2881 cgggtgcttt ggccatagcc gcgaagctgc gcctgggcaa tctcgggccg ccccaggtgg
     2941 ttcgggagac cggccaggcc atcgtcttcg acctcggaat cggcgagtgg catgccgtct
     3001 tggcgcaaaa gcgtcacccg cgccgcacac agaggcgcaa gggcccgcgc ggtctcttgc
     3061 cagcgcttgg gatcggacgg ctgcaggcgg gccgtctcgg cggagtgggc cacaagccgc
     3121 agccgctggg tcaggtcttg ctcgatctgc gtcgtcaggt tggcatccag cgtccgccgc
     3181 aggtagccat aggaaatccc gagcgacagc gcgatcagcg acagcgacaa aaaaaacagc
     3241 ttgagacgaa tgcccagctt cacggcctac gatccttgga ctgaaccatc aaacggggtg
     3301 cggaagcgat acccgacgcc acgcacggtt tcgatcagct cgccttcgtc gccaatcttt
     3361 tcgcgcaggc gctttacgtg ggtgtcgacg gttcgcgtgg tgatggcgcc ttcgacgttc
     3421 cagtcttgcc acgcggtcga cagaagctgg gcgcggctta gcactttgcc gtgctgactg
     3481 gcgagcgtcc acagcagacg aaactccagc gcggtcagct tcttttcttc gctgccaaca
     3541 aacacccggt gcgccgatcg atcgatgcgc aacgcaccaa tcgtgatc
//
LOCUS       DAORGN010000321         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_14936_length_3583_cov_4.110261, whole genome shotgun sequence.
ACCESSION   DAORGN010000321 DAORGN010000000
VERSION     DAORGN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14936_length_3583_cov_4.110261"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..951
                     /locus_tag="PKL17_21080"
     CDS             202..951
                     /locus_tag="PKL17_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNK47290.1"
                     /translation="MSVFQILVLDADPWTVKMATSLLQRRGHTVLVASSTDEAAQQIT
                     QAAVDLLIVDAQLNGSDLGFVAKLRGLVSGRVLPVLLLAESAPPALRIDALRVPHSDF
                     LDKPFRFDEFDLRVDRLLVREPPQVQAPAVPEAGLRGTLGELSLATLLLLIESEQKSG
                     VLTVQRPGEVGRMWCAKGRLLSGERTIDGQKPERSMDAVFELLSWQSGSFDFVAGPQP
                     CDDDEGLCITQLVLLLAQRQDEDTRQQSIAV"
     gene            967..1593
                     /locus_tag="PKL17_21085"
     CDS             967..1593
                     /locus_tag="PKL17_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47291.1"
                     /translation="MRRSGCHAVLGLWLVGLPTVSLAAPPVLPSTQPQSPPALPPRPG
                     QPLPNVVPLPNLPPSPTGDSGLSGETDVTVIAPPSPTVQNVMPILTTERRRRFNLLIA
                     GVSTLLAAYTADRLLARDLSQSPVSWVPLVGPWWILNEETLRPNPSSGLQALLVVDGI
                     VQLSGLALTFVGTFLRYDRVVLRLPSPSETSLIPAPPLAPPAPADTTR"
     regulatory      1636..1730
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1771..3531
                     /gene="thiC"
                     /locus_tag="PKL17_21090"
     CDS             1771..3531
                     /gene="thiC"
                     /locus_tag="PKL17_21090"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNK47292.1"
                     /translation="MTERNPLPLRGAVNPSSRSAGTSPGSFANAADLGGPLSFSSPDT
                     PGMPAASGKTAWDFMPPDWKLVDGKWTPPSGFVPVTQLEHARLGIVTPQMRRVAEREP
                     HLTPEQVRDEVAAGRLIIPANRIHLQYKLDPMAIGRASRTKVNANMGASPVSSSTQEE
                     VEKLRWAEKWGADTVMDLSTGGDLDACRQAIVQNATVPIGTVPIYSMIIGRKIETLTP
                     QIILDSLRQQAAQGVDYFTIHAGVLRSHLPLLRDRLIGIVSRGGSLLAKWMLHHHAEN
                     PMYTLFDDICAIMREYDVSFSLGDGLRPGGLGDATDAAQLAELATLGQLTERAWRHGC
                     QVMVEGPGHVPFDQIEFNMKLQRRLCHGAPFYVLGPIVTDVFPGYDHITSCIGATAAA
                     YHGAAMLCYVTPKEHVGLPKKDDVKQGCVAYKIAAHAADVALGIPGTRDWDDDLTQAR
                     AALNWEKHFALAFDGEYARALHDEDLDVDTDFCAMCGHDWCSVRISKEIVEFTSGKAP
                     GFARDGVTKSAALSEQQQEVLRSRGVLSQEEIHRLAHKTRKAVGAENGKAVCHSDYLD
                     SQAAERLQGEKLVQIGRRAQ"
BASE COUNT          679 a         1032 c         1143 g          729 t
ORIGIN      
        1 tgttacccgc tagcacctca agctagtgcg tctaccagtt ccgccacctt cgcggtttga
       61 tcgcttcgtt tggggtgaag cgccaacagg acggtaatta gccgtaatca ggggcgatct
      121 gtcaaggcat cttttgtggg cggtttgttt tttgtgaaga ttgcggctca ttgtcgtcgg
      181 aaaccgagta aggtacgtcc gatgtcggtt tttcagattt tggttttgga tgcagatccg
      241 tggacggtga agatggccac gtcgcttttg cagcggcgcg gtcatacggt cctggttgca
      301 agctcgacgg atgaagcggc gcagcagatc acgcaggcgg cggttgacct gctgatcgtc
      361 gatgcgcaac tgaacgggtc cgatctgggc tttgtcgcca agctgcgagg gctggtcagc
      421 gggcgtgttc ttcctgttct gctgctcgcc gagtcggcgc cccctgccct gcggatcgat
      481 gcgctgcggg tgccgcacag tgactttctc gacaagccgt ttcgctttga tgaatttgat
      541 ctgcgcgtcg atcgactgct ggtgcgtgaa ccgccgcaag tacaagcgcc ggctgtgcca
      601 gaagcgggtc tgcgcggaac actcggcgag ctgagcctgg cgacactgct gcttctgatc
      661 gaatcggaac agaagagcgg cgtactgacg gtgcaaagac cgggtgaagt cggtcgaatg
      721 tggtgcgcca agggtcgcct gctgagcgga gagcggacga tcgacggtca aaaacccgag
      781 cgcagcatgg acgccgtgtt cgaacttctg tcgtggcaga gcggatcgtt tgattttgtc
      841 gccggtccgc agccgtgtga tgacgacgaa ggactttgca tcacgcagct tgttttgctg
      901 ttggcgcagc ggcaagacga agacacacgg cagcagtcaa tcgccgtatg atgggggcac
      961 ttgcctatgc gacgctctgg ctgccacgct gtgctgggac tttggcttgt cggtctgccg
     1021 accgtgagcc tggcggctcc ccccgtgttg ccgagtacgc agccgcagag tccgcccgcg
     1081 ttgccgcccc gtccgggtca gccgcttccg aatgtggtgc cgctgcccaa cttgccaccg
     1141 tcgccgaccg gggacagtgg gctgtctggg gaaaccgatg tgaccgtgat tgcgcctcca
     1201 tccccgacag ttcagaacgt gatgccgatc ttgacgaccg aaagacggcg ccggttcaac
     1261 ttgctgattg ccggcgtgtc gacgctgctt gcggcctata ccgcggatcg gctgctcgcg
     1321 cgggatctgt cgcagtcgcc ggtcagctgg gtgccgctgg tcggtccgtg gtggatcttg
     1381 aacgaagaga cgctgcgacc aaacccaagc tctggccttc aggcgctctt ggtcgtcgat
     1441 ggcatcgtgc agcttagtgg tctggcgctg acgtttgtcg gaaccttttt gcgctacgac
     1501 cgcgtggtgc tgcgtctgcc gtcaccgagt gaaaccagcc tgatccctgc acccccgctt
     1561 gcgccgccag cgcctgcgga cacaacgcgc tagacattgc tatccgcgga ccttcatttg
     1621 cgctagattc ggccccggaa tcgctcgggg cgcccaaaaa caagggctga gatgcacccg
     1681 cagaacctga tccggatcat gccggcggag ggaagcgaca tcgaagcgcc cgtcggccac
     1741 ccgtgattcc gaaagtctct ggaggaactc gtgaccgaac gcaatcccct tccccttcgt
     1801 ggtgccgtca atccgtcttc gcgcagtgcc ggaacgtccc ctggatcctt tgcaaacgct
     1861 gccgacctgg gtggaccgct cagcttttca tcgcccgata cgccgggaat gccagcagca
     1921 agtggcaaga ccgcgtggga ttttatgccg ccagactgga aactggtcga cggaaaatgg
     1981 acgccgccgt cgggttttgt cccggtgacg cagctagagc atgcgcggct tggcatcgtg
     2041 accccgcaga tgcgtcgcgt cgccgagcgg gaaccgcatc ttacgcccga gcaagtgcgc
     2101 gacgaagtgg ccgcgggacg gctgatcatt ccagccaacc gcatccatct tcagtacaag
     2161 ctcgatccga tggcgattgg ccgcgcgagc cgcaccaaag tcaacgccaa catgggcgct
     2221 tcgcccgtga gttccagcac gcaagaggaa gtcgaaaagc tccgctgggc agaaaagtgg
     2281 ggcgccgata cggtgatgga tttgtcgacg ggcggcgacc tggatgcctg ccgccaagcg
     2341 atcgtgcaaa acgccacggt gcccatcgga acggtgccga tttattcgat gatcattggt
     2401 cgcaagatcg aaacgctgac gccgcagatc atcctcgatt cgctgcgtca gcaagcggcg
     2461 caaggtgtcg attacttcac gattcacgcg ggcgtgttgc gttctcacct gccgcttttg
     2521 cgtgatcgcc tgattgggat tgtgtcgcgc ggtggatcgc tgttggccaa gtggatgctg
     2581 catcaccacg cggaaaatcc gatgtacacg ctgttcgacg acatctgcgc catcatgcga
     2641 gagtatgatg tttcgttttc gctgggcgac gggctgcgtc cggggggact cggcgatgcg
     2701 accgatgcgg cgcagcttgc ggagctggca acgctcggac agcttacgga acgcgcgtgg
     2761 cgacacggct gccaagtcat ggtcgaaggt ccaggtcacg ttccgttcga ccaaatcgag
     2821 ttcaacatga agctccaaag gcggctctgt cacggcgcgc cgttctatgt gctggggccg
     2881 attgtgaccg atgtatttcc tggctacgac cacatcacaa gctgcatcgg tgcgacggca
     2941 gcggcgtatc acggggcggc gatgctctgt tatgtgacgc ccaaagagca tgtcggtctg
     3001 cccaaaaaag acgatgtgaa acagggctgt gttgcctaca aaattgctgc gcacgccgcc
     3061 gatgtggcac ttgggattcc gggtacgcgc gactgggatg atgaccttac gcaagcccgc
     3121 gcggcgctca actgggaaaa gcactttgcg ctggcattcg acggagaata cgcgcgcgca
     3181 ctgcacgatg aagacctgga tgtggacacc gatttttgcg cgatgtgcgg gcatgactgg
     3241 tgttccgttc gcatctcgaa agagatcgtt gagttcacat ccggaaaagc gccaggcttt
     3301 gcccgcgacg gtgtcaccaa gtctgcggcg ctttcagaac agcaacagga agtgctcaga
     3361 tcgcgtggtg tgctgtccca agaagagatc caccgcttgg cccacaagac ccgcaaagcc
     3421 gtcggtgcgg aaaacggaaa agcggtctgt cacagcgact atttggattc ccaagcagca
     3481 gagagactgc aaggggaaaa gctggtgcag atcggaagac gcgcacagtg attcccaacg
     3541 tacttacggt agcgggcgtg gacccaagtg gcggtgctgg gat
//
LOCUS       DAORGN010000322         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_16245_length_3414_cov_4.875558, whole genome shotgun sequence.
ACCESSION   DAORGN010000322 DAORGN010000000
VERSION     DAORGN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16245_length_3414_cov_4.875558"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1094)
                     /gene="lipA"
                     /locus_tag="PKL17_21095"
     CDS             complement(129..1094)
                     /gene="lipA"
                     /locus_tag="PKL17_21095"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNK47293.1"
                     /translation="MHSTPQSPRHLPIVGSGAPSQADGTADRPSEPRPRHPAWIKARL
                     PSGKTFFEVRDLVHGQKLHTVCESASCPNIGECWSRRAITIMILGNICTRSCQFCDVP
                     TGRPLPPDPDEPRRVALALKELGLHHTVITCVDRDDLKDGGAAHWAETIRQVKTHCTG
                     MTLEVLTGDFKGNTGDVDTVLTASPDVFAHNLETVPRLSRQVRVQASYPRSYKILAHA
                     KSRGAVTKTGLMLGLGEELSEVRDVLRELAALGIDIVTMGQYLRPSKSHLPLARYISP
                     EEFAELREYALALGIPHVESGPLVRSSYHADGQADLVRALRKSTT"
     gene            complement(1094..2539)
                     /gene="lpdA"
                     /locus_tag="PKL17_21100"
     CDS             complement(1094..2539)
                     /gene="lpdA"
                     /locus_tag="PKL17_21100"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNK47294.1"
                     /translation="MKAQIGQAPASSGPDVVVVGGGPGGYVAAIRAAQLGKKVLVVEK
                     AHLGGICANWGCIPTKALLHSAELYAGLKKGAALGVVADGLRFDYPAAISHSRKVAEN
                     QKKGVGLLFKKNQIEHIDKTATLRRESGKLKLFVDGKELVAPHIIIAVGAKPKTIPGL
                     VPDGKDILTYFEAMNLPSQPKSLLVVGAGAIGIEFAYFYNAIGTEVTVLEAMPQILPV
                     EDSEVAEQLKRELGKQGIRIHVGARFAGCEKVGGQQKVRFTDPNGQPQEIIVDKVLSA
                     VGVTGNLESIGAAQVGVKIDRGFLAVDEWYRLLDQNGKPEPGFYAIGDCIGGPLLAHK
                     ASAEGICCVEKIAGIPEREIRRVDLSSMPGATFCRPEVGSMGLTEQKARADGRKIKIG
                     KFPFKASGKAQATGETEGFVKVVLDDVTGEILGAHILGGTASDMISALCLARGGELTA
                     TEVLHTVLPHPTYGEIMKGAFEAAFGEAIDL"
     gene            complement(2539..>3414)
                     /locus_tag="PKL17_21105"
     CDS             complement(2539..>3414)
                     /locus_tag="PKL17_21105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNK47295.1"
                     /translation="PLARRVAADLGVDLRTLKGTGPGGRIVKRDVEAAPRGATWKTSA
                     PATIPAPVQLLPGDELAPLSMIRKTAAKRLIEAKQTVPHFYLTSDVDMEAAMAFREQL
                     NAASQASGGEKVSVNDLILKALARALRLCPRANMSVSPDGQSAIAHQRVDVSVAVALD
                     DGLITPVVRGADGKSLGALAKEVRELAARGRDKKLKPEEYQGGTFSLTNLGMYGIREF
                     FAIINPPESGILAVGRVEKRAVVVERDGKDHIESRRMLTLTLSCDHRIVDGALGAQLL
                     AKVVEGLAKPMLLVL"
BASE COUNT          586 a         1077 c         1065 g          686 t
ORIGIN      
        1 cacgccagcg cagccatatc gtccggcgcc gccgtatcag cccgcgccgc cgtatcagcc
       61 cgcgccgccg taccagcctg cgccgccgta tcagccgccg ccgtcgggat actagaccgg
      121 caaaaacgct acgtcgtgct cttgcgcagg gcgcgcacca ggtcggcttg gccgtcggcg
      181 tgatacgacg aacggacgag cggcccggac tcgacgtggg gaatccccag cgccagcgcg
      241 tactcgcgca gctcggcaaa ttcctcggga gagatgtagc gcgcaagcgg cagatggctc
      301 ttcgacggac gcaggtactg gcccatcgtc acaatgtcga tgccaagcgc ggccagctcg
      361 cgcagcacgt cacgcacttc ggaaagctct tcgcccaggc ccagcataag gcccgtcttg
      421 gtgacggcgc cgcgcgactt ggcatgggcc aaaatcttgt aactgcgcgg gtagcttgcc
      481 tggacgcgca cctgacggga cagccgcggc acggtttcca ggttgtgagc aaacacatcg
      541 ggtgaagcgg tcaggacggt atcgacgtcg cccgtgttgc ccttgaagtc gccggtcagc
      601 acttcgagcg tcatgccggt gcagtgggtc ttgacctggc gtatcgtctc ggcccagtgc
      661 gccgcaccgc cgtctttcag gtcgtcgcga tcgacgcagg tgatcacggt gtggtgaagt
      721 cccagctctt tgagcgccag cgcgacgcga cgcggctcgt cgggatcagg gggcagcgga
      781 cgcccggtcg gcacatcgca aaactggcac gaccgtgtgc agatattgcc caggatcatg
      841 atggtgatgg cgcgtcggct ccagcactcg ccgatgttcg ggcagctcgc ggactcgcag
      901 acggtgtgca gtttttgccc gtggaccaga tcgcggactt caaaaaacgt ctttcccgac
      961 ggcaatcgcg ctttgatcca ggccggatgg cgtgggcgcg gctcgctggg gcgatcggcg
     1021 gttccatcgg cctgagacgg cgcgccactt ccgacgatgg gcagatgacg cgggctttgc
     1081 ggtgtgctgt gcattacagg tcgatggcct cgccgaaggc tgcctcgaaa gcgcctttca
     1141 tgatttcgcc ataggttgga tgcggcaaga cggtgtggag cacttcggtc gcggtcagct
     1201 cgccgccgcg tgccaggcac agcgccgaaa tcatgtcgct ggcggtgcct cccaggatgt
     1261 gcgcgccaag gatttcgccg gtcacatcgt cgagcacgac tttgacaaag ccttcagttt
     1321 cgccggtcgc ttgtgctttg cccgacgctt tgaaggggaa cttgccgatc ttgatcttgc
     1381 gaccgtcggc gcgggctttc tgttcggtca ggcccatcga tccgacttcg ggacggcaga
     1441 acgtcgcacc gggcatgctg ctcaggtcga cgcgacggat ctcgcgttcg gggatgccgg
     1501 cgattttttc gacgcagcag atgccttccg ccgacgcttt gtgggcgaga agtggaccgc
     1561 cgatgcagtc gccgatcgcg taaaagccgg gctcgggttt gccattttga tcaagcaggc
     1621 gataccactc gtcgacggcg aggaagccac gatcgatctt taccccgacc tgggcggcac
     1681 cgatcgattc gagattgccg gtcacgccga ctgctgaaag cactttgtcg acgatgattt
     1741 cctgcggctg gccgtttgga tcggtgaagc ggactttttg ctgaccgccg actttttcac
     1801 agccagcaaa gcgcgcacca acgtggatgc ggatgccttg tttgcccagt tcgcgcttta
     1861 gctgctcggc gacttcgctg tcttcgacgg ggagaatctg cggcatcgct tccagcaccg
     1921 tcacttctgt gccgatcgcg ttgtaaaaat aggcaaattc gatcccaatc gcacctgctc
     1981 cgacgacgag cagcgacttc ggctggctcg gcaggttcat cgcttcaaag taggtcagga
     2041 tgtctttgcc gtcgggaaca agccccggaa tcgtcttggg tttggccccc accgcgatga
     2101 tgatgtgcgg cgcgaccagt tctttcccgt cgacaaatag ctttagcttg cccgactctc
     2161 gccgcagcgt cgctgtcttg tcgatgtgtt cgatctggtt ctttttgaac agcagaccga
     2221 cccctttttt ctggttttca gcgactttgc ggctgtgcga aatggccgct ggatagtcaa
     2281 aacgcaggcc atcggcgacg acaccaagcg ctgcgccctt tttcagcccc gcgtaaagtt
     2341 ccgccgagtg aagcagcgct ttggtcggga tgcagcccca gttggcacaa atcccgccca
     2401 gatgcgcttt ttcgacgacc agcacttttt tgccaagctg ggcggcgcga attgcggcga
     2461 cgtacccacc tgggccccca ccgacgacca caacatctgg accggacgac gccggagctt
     2521 ggccaatctg ggctttcatc acagcaccag cagcatcggc ttggccaggc cttcgacgac
     2581 cttggcgagc agttgcgcac cgagcgcacc gtcgacaatc cggtgatcgc acgacagcgt
     2641 cagggtcagc atgcggcgcg attcgatgtg gtctttgcca tcacgctcga cgacgactgc
     2701 gcgtttttca acccgtccga ccgccaggat gccgctttcc ggtgggttaa tgatcgcgaa
     2761 aaattcacgg atgccgtaca tgcccaggtt ggtcaggctg aaggtgccgc cctggtattc
     2821 ctcgggcttt agctttttgt cgcgaccacg ggccgccagc tcgcgcactt cttttgccag
     2881 cgcaccgagc gatttgccgt cggcaccacg caccaccggc gtgatcaggc catcgtccag
     2941 cgcgaccgcg acgctgacat cgacgcgctg atgcgcaatg gcgctttgtc cgtcgggaga
     3001 cactgacatg ttggcgcgcg gacacagccg cagcgcccgc gccagggctt tcagaatcag
     3061 gtcattgacc gacacttttt cgccgccgct cgcttggctg gccgcgttaa gctgttcgcg
     3121 gaatgccatc gcggcttcca tgtcgacgtc gctggtcaga tagaagtgcg gcaccgtctg
     3181 cttggcttcg ataagccgct tggccgccgt cttgcgaatc atcgacagtg gggcaagttc
     3241 gtcgcccggt agaagctgga cgggagcggg gatcgtcgcg ggcgcactgg tcttccatgt
     3301 ggcaccgcgg ggcgctgctt cgacatcacg tttgacaatg cgaccaccgg ggcccgtgcc
     3361 tttgagcgtg cgcagatcga cacccaaatc ggcagccacc cgtcgggcca gcgg
//
LOCUS       DAORGN010000323         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_16457_length_3388_cov_6.255026, whole genome shotgun sequence.
ACCESSION   DAORGN010000323 DAORGN010000000
VERSION     DAORGN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16457_length_3388_cov_6.255026"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..2663
                     /locus_tag="PKL17_21110"
     CDS             219..2663
                     /locus_tag="PKL17_21110"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNK47296.1"
                     /translation="MDEQPPPLPTVCPDLPAPPSGQRCTATASPTGSKAILLSGTLLL
                     PDSTMKNGQVLVDSTGVIACVSCDCSSHPEAAAASTVSCPDGVISPGLINTHDHITYT
                     KAEPGTHPTNRYNHRHEWRIGVAGDPKRPKISVPGSNNSSAVQWGELRQLMGGTTSLV
                     GSGSAKGVLRNLDTNVQEGLTEKPVDFDTFPLGDSGGERLTSGCDYPSIHKSTALQTV
                     DAYEPHISEGINDEAHNEFLCTSATLYGGQRVVEDKTAMIHAIGLTANDAWLASARGA
                     SVIWSPRSNISLYGHTAQVPMMAKVGVSIALGTDWTASGSINLLRELSCAADLNEKQF
                     GKFFTARDLWQMVTFNAALATATNDVLGQIAVGKIGDLSIFISKPDRTEHQAVVRAGV
                     EDVALVLRSGLPMYGDATVLEALGANDAGKCEVLDVCGVSKRICAERETGKKLADLET
                     AAIKPIYQLFGCGIPSKEPTCVPYREGEFDGMSTMDDPDGDGIKGAADNCPAVFNPIR
                     PMDRGIQPDTDGDGIGDACDLCPLDKDNTMCRKPDANDEDGDGISNNIDNCSTVSNAD
                     QKDSDMDGQGDLCDACPNFKNPAGAPCEFSVKDLRDPARGLRPPVGTKVMVKDLLIVG
                     LRNIKSFGFHARDVGTDLPYSGILVFQGGTAAPKATDGTPLQVGHIVSVTGNFTVFSE
                     QDEIDTVTNVTITGMDPTAAGVFVDVKTRDLLGGQQTTAERFENLLVRVKSVTARNAL
                     TSDDDFWVTDEATEMCTGTNPGCTRVGDFLLDGDKTDGSPKFTPGTALSEIQGIVSGF
                     GNQYTLMPKALTDIKP"
     gene            complement(2765..>3388)
                     /locus_tag="PKL17_21115"
     CDS             complement(2765..>3388)
                     /locus_tag="PKL17_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNK47297.1"
                     /translation="TRKGITSVQMDIKIPGLSREVLVRALKQARDGRLYILDKMAQTI
                     ATPNKEMSKYAPRIYRLQVKPDRVRDIIGPGGKMIRAIIEQTGVAIDIEDDGTVNIAS
                     PDGPSAQKAIDIVKGLTAEPEVGSFYMGTVRRIVEFGAFVEILPGTDGLVHISELANE
                     RVHNVADVVKEGDEVLVKVVGIDRTGKIRLSRKEALGQQPDQVLNMR"
BASE COUNT          710 a         1061 c         1010 g          607 t
ORIGIN      
        1 ctagccgagt ggtggcggca gtgtagtttg cgtgcatccg gcggtaaaac gaaaaagtaa
       61 ctttggcgca agctggggcc gacctacgat atgcccgagt ccagagccac cccctaggag
      121 agagcccgat gcaaaccgta cgtaggcaac gccttctgcg ccttgcgacc ctttcgctgc
      181 ttctacttct ccccgtctgt gatcgcagtc cccagacgat ggatgagcaa ccgccaccgc
      241 tgccaacggt gtgtcccgat ctgccggccc cgccgagcgg acagcgctgc accgcgaccg
      301 caagtccgac gggaagcaaa gcgattttgc tcagtggcac gctgcttctg cccgattcga
      361 cgatgaaaaa tggccaagtg ctggtcgatt cgacgggtgt gatcgcgtgc gtatcgtgcg
      421 actgctcaag ccacccagaa gccgccgccg catcgacggt gagctgtcct gacggcgtga
      481 tttcacccgg cctgatcaac acgcacgatc acatcacgta caccaaggcc gagccaggca
      541 cccacccgac gaaccgctac aaccaccggc acgagtggcg gatcggcgtt gcgggcgatc
      601 ccaagcggcc caagatttcc gtcccaggca gtaacaactc gtcggcggtc cagtggggcg
      661 agctgcggca gctgatgggc ggcaccacgt cgctcgtcgg atcaggatcg gccaaaggag
      721 tgcttcgcaa cctcgacacc aacgtgcaag aaggtctgac cgaaaagccg gtcgattttg
      781 acacgtttcc cctcggtgac tcgggcggtg agcggctgac ttcaggctgt gactatccgt
      841 cgatccacaa gtcgaccgcg ctgcaaaccg tcgatgccta cgagccgcac atcagcgagg
      901 gcataaacga cgaagcccac aacgaatttc tgtgcaccag cgcgacgctg tacggtggcc
      961 agcgcgtcgt cgaagacaag acggcgatga tccacgcgat tggacttact gcaaatgatg
     1021 cgtggctcgc gtcggcgcgt ggtgcgtcgg tgatctggtc gccgcgcagc aacatttcgc
     1081 tgtatggaca caccgcgcag gtgccgatga tggcgaaagt gggcgtctcg atcgcgctcg
     1141 gtacggactg gaccgcgtcg ggatcgatca accttctgcg cgagctgtcg tgcgctgccg
     1201 acctgaacga aaagcagttt ggcaagttct ttaccgcgcg tgacctgtgg cagatggtga
     1261 cgtttaacgc ggcactggcg acggcgacga atgacgtgct gggacagatt gcggtcggca
     1321 agatcgggga tctgtcgatt ttcatcagca agccggatcg cacggaacac caagccgtcg
     1381 tgcgggcggg cgtcgaagat gtcgcgctgg tgctgcgcag cggcctgccg atgtacgggg
     1441 atgcgacggt gctcgaagca ctgggcgcaa acgacgccgg caagtgtgaa gtgcttgatg
     1501 tctgtggcgt gtcgaagcgg atctgcgccg agcgcgagac aggcaaaaag ctggctgact
     1561 tggaaaccgc agcgatcaag cccatctatc agcttttcgg atgtggcatt ccgtcgaaag
     1621 aaccgacctg cgtgccctat cgcgagggtg agttcgacgg catgagcacc atggatgatc
     1681 ccgacggtga tggaatcaaa ggcgccgccg acaactgccc cgccgtgttt aacccgattc
     1741 gaccgatgga tcgcggcatt cagccagaca ccgacggcga tggcattggc gatgcgtgcg
     1801 atctctgtcc gctcgacaaa gacaacacga tgtgccgcaa gccggacgca aacgacgaag
     1861 acggcgacgg catcagcaac aacatcgaca actgctcgac ggtatcgaac gccgaccaaa
     1921 aagacagcga catggacggc cagggcgatc tttgcgatgc ctgtccgaac ttcaaaaatc
     1981 ccgccggcgc gccgtgtgaa ttttcagtca aagacctgcg cgatcccgcc cgcggtctgc
     2041 gtcctcccgt cggtaccaaa gtcatggtga aagacctgct gatcgtcggc ttgcgcaaca
     2101 tcaagagctt cggtttccat gcccgcgacg tcggcaccga cctgccctat tcaggaatcc
     2161 tggtgttcca aggcggcacc gcggccccga aagccaccga cggcacgccg ctgcaagttg
     2221 gccacatcgt cagcgtcacc ggcaacttca ccgtctttag cgagcaagac gaaatcgaca
     2281 ccgtcacaaa tgtcaccatc accggcatgg acccgacggc agccggcgtg ttcgtcgatg
     2341 tcaaaacccg cgatctgctc ggcggacagc agaccacggc agaacgtttt gaaaacctgc
     2401 tcgtgcgtgt caagagcgtg accgcccgca atgcgctgac cagcgacgat gatttctggg
     2461 tcacagacga agccaccgag atgtgcaccg gcacaaatcc aggctgcacc cgcgtcggcg
     2521 acttcctgct cgatggcgac aagaccgacg gcagccccaa attcacgccg ggaaccgcgc
     2581 tcagtgaaat ccagggcatc gtgagcggct ttggcaacca gtacacgctg atgccaaaag
     2641 cgctcaccga catcaaaccg taagctcatc ccagccccgc acggggcatg atcccatcgc
     2701 ccaaaacaaa aaaccccgca cccgaatcga tcggggcggg gtctttttgc gaaagaagca
     2761 gcgcttagcg catgttgagg acttgatcgg gctgctgccc gagcgcttcc ttgcgcgaaa
     2821 gccgaatctt gccagtgcga tcgattccga cgaccttgac cagcacttca tcgccttctt
     2881 tgacgacgtc ggcgacgttg tggacgcgct cgttggccag ctcgcttatg tgaaccagac
     2941 cgtctgtgcc cggcaggatc tcgacgaacg caccaaactc gacgatgcga cgaacggtac
     3001 ccatgtagaa gctgccgact tccggttccg ccgtcagccc cttgacgatg tcgatcgcct
     3061 tctgcgccga cggtccatcc ggcgaggcaa tgttcaccgt gccgtcgtcc tcgatgtcga
     3121 tcgcaacacc ggtctgttcg atgatcgcgc ggatcatctt gccgccgggg ccgatgatgt
     3181 cgcgaacgcg gtcgggcttg acctggagac ggtaaatccg cggcgcgtac ttcgacattt
     3241 ccttgttcgg cgtcgcgatg gtctgcgcca tcttgtcgag gatgtacagc cgtccgtcgc
     3301 gcgcctgttt gagcgctcgc acaaggactt cccgcgacag acccggaatc ttgatgtcca
     3361 tctggacgct ggtgatgccc ttgcgcgt
//
LOCUS       DAORGN010000324         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_16540_length_3380_cov_4.050226, whole genome shotgun sequence.
ACCESSION   DAORGN010000324 DAORGN010000000
VERSION     DAORGN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16540_length_3380_cov_4.050226"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..>3380
                     /gene="hsdR"
                     /locus_tag="PKL17_21120"
     CDS             312..>3380
                     /gene="hsdR"
                     /locus_tag="PKL17_21120"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:NF008521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HNK47298.1"
                     /translation="MTPKPSPNFAYLAYHDARLSALATQAEEHFAGDPTVTLFKLRQF
                     GEVLAQRAAAKVGLFVGAAEPQKQLVDRLLERGVIGATQCLLFHDLRRVGNAAVHEGK
                     GSHSDALHQLRMARELAVWFQRSFGSNQKFDPGPFVPPAGPGKPASALADELARLRSD
                     VEAHKQDLLAAQRAIEDARKAAEAELSAKLTAQERAAKAQEDAAIWEVLASEQIETHR
                     ATAKAESQKSAALAAQNQKLQAELAALSTAAQAMPPRQLAKTIERAAEASEAIELDEA
                     ATRKIIDQQLRRAGWEVDTERLTFAAGVRPQKGKNLAIAEWPTLADGEEGRADYVLFA
                     GLVVVAIVEAKRKHKDISGVIPQAKRYSVGYVIHANETLPDGSPWGACKVPFLFATNG
                     RPFLRQLQTKSGIWFLDVRRAANHPVPLEDFYTPEGLLDRLRQDIDAAHARLSVEPMP
                     YIDREYQKKAILAVEHEIAQGKRELLVAMATGTGKTRTCIGLCYRLLKTRRFRRILFL
                     VDRNALGKQTADALKDLRLENLQTFTDIFNVKELADLVPDRETKLHIATIQAMVRRVL
                     GASDDRRAPEFPPVDQYDCIIVDECHRGYLLDRELSDREFEFRDEADYISKYRRVLDH
                     FDAVKIGLTATPALHTKEIFGTPVFSYSYREAVIDGFLVDHEPPIRIVTQLAHDGIHY
                     SAKEEVLVLDRATQTVHKEELADEVSFDVDAFNRRVITEPFNRAVLGELVNHIDPEQD
                     EKTLIFCASDAHADLVVVLLKEALAKKYGAVDNDAVLKITGQTDRPLDAIRRYRNERQ
                     PSIVVTVDLLTTGIDVPKIANLVFLRRVRSRILYEQMIGRATRLCPEIGKERFRIFDA
                     VDLYSALKDVTDMKPVVVNPLVSFAQLAQEVREGKEEAPRRASIDEFVAKLQRKKRML
                     LRDAEEQLATAAGMGLRDLVQFLKSARVAEVADYLTAHPALAAFLDRATGSGANKVIV
                     SEKSDAIHEVSRGYGPGVSGRPGDYLESFRAFVSGSLNRLPALLVVTQRPK"
BASE COUNT          668 a         1025 c         1103 g          584 t
ORIGIN      
        1 cgtgaaccgc cttcgcagca agccagcggc gcagctcgaa ccagcgcccc ggggagctgc
       61 acaaacgctg gtgagtggtg atttcctacg gcctgctgtt tgagcctgtt cagcgtaggg
      121 cagcacagcg cggcgaccgt ttggagtgga aggtcctctg tcgcgcatcg ctgagcaagg
      181 agcgactcgc tgcggtcttc acacccaacg gctgctcgca ttccccaggt agcgagtgag
      241 ccagactcgt gtagcgtccc aagcccaggc cctcttgtca aagtgagcgt ttgtgacgac
      301 tatcctgcgg cgtgacgccc aagccttccc cgaactttgc ttaccttgcc tatcacgacg
      361 cgcggctttc ggcgctggcg acgcaggcgg aagagcactt tgcgggcgac ccgacggtga
      421 cgttgttcaa gctgcggcag tttggcgaag tgctggcgca gcgggcggcg gccaaggtcg
      481 gactgttcgt tggggcggca gagccgcaga aacagctcgt cgaccgcttg ctggaacgcg
      541 gggtgattgg ggcgacgcag tgcctgctat ttcacgacct gcggcgcgtc ggcaatgcgg
      601 cggttcacga aggcaaaggc agccacagcg acgcactgca ccagcttcgg atggcgcgag
      661 agctggcggt gtggtttcag cgcagcttcg gcagcaatca gaagtttgat ccggggccgt
      721 ttgtgcctcc ggcgggaccg ggcaagccag cgtcggcgct tgcggacgag cttgcccgcc
      781 tgcgatccga cgtcgaggcg cacaaacagg atctgctcgc ggcccagcga gccatcgagg
      841 acgcgcgcaa ggccgccgag gccgagcttt ccgcgaagct gacggcgcag gaacgggcgg
      901 cgaaggcgca ggaagacgcg gccatctggg aagtgctcgc gagtgagcag atcgaaaccc
      961 accgcgccac cgcgaaagcc gagtcacaaa agagcgcagc gctcgcggca cagaaccaaa
     1021 agctccaggc cgagctggca gcgctttcga cggcggcgca ggcgatgcca ccgaggcagc
     1081 ttgcgaagac catcgagcga gccgccgagg cgagcgaagc gatcgagctc gacgaagcgg
     1141 cgacgcgcaa gatcatcgac cagcagcttc gtcgcgccgg ttgggaagtc gacaccgagc
     1201 gcctgacgtt tgcggcgggc gttcgtccgc aaaagggcaa aaacctggcg attgccgagt
     1261 ggccgacgct ggcagacggg gaagaaggcc gcgccgacta tgtgctgttt gccggtctgg
     1321 tggtggtcgc catcgtcgag gccaagcgca aacacaaaga cataagcggc gtgattccgc
     1381 aggccaagcg ctattccgtc ggctacgtca tccacgcaaa cgagacgctt cccgacggca
     1441 gcccgtgggg cgcatgcaag gtcccgtttc tgtttgcgac caacgggcgt ccgtttttgc
     1501 ggcagcttca gaccaagagc ggaatctggt ttctcgacgt gcggcgggcg gccaatcatc
     1561 cggttccgct cgaagacttt tacacgcccg agggactgct cgaccggctt cggcaagaca
     1621 tcgacgccgc gcacgcccgg ctttccgtcg agccgatgcc gtacatcgat cgcgaatacc
     1681 agaaaaaagc gatcttggcc gtcgagcacg agattgccca aggcaagcgc gagctgctcg
     1741 tggcgatggc gacgggaacg ggcaagacgc gcacctgcat tgggctgtgt taccgactgc
     1801 tgaagacgcg gcgctttcgg cggattctgt ttttggtcga ccgcaatgcg ttgggcaagc
     1861 agaccgccga cgcgctgaaa gacctgcggc tggaaaatct tcagacgttt accgacatct
     1921 tcaacgtgaa agagctggcc gaccttgtgc ccgaccgcga gacgaagctg cacatcgcga
     1981 cgattcaggc gatggtgcga cgggtgctcg gggccagcga cgacagacgc gcgccagagt
     2041 ttccgcccgt cgaccagtac gactgcatca tcgtcgacga gtgccaccgt ggctacctgc
     2101 tcgaccgcga gttgtccgac cgcgagtttg agttccgcga cgaggccgac tacatctcga
     2161 aataccgtcg ggtgctcgac cacttcgatg cggtgaagat cggtctgacg gcgacgcccg
     2221 ctctgcacac aaaagaaatc tttggcacgc cggttttttc gtattcctat cgcgaagcgg
     2281 taattgatgg ctttctcgtc gaccatgagc cgccgattcg catcgtcacc cagcttgcgc
     2341 acgatggcat tcactacagc gccaaagaag aggtgctggt cctcgaccga gcgacgcaga
     2401 ccgttcacaa agaggagctg gccgacgagg tgtcgttcga tgtggatgcg ttcaatcgcc
     2461 gcgtcatcac cgagccgttc aatcgcgcgg tgctcggcga gctggtgaat cacatcgacc
     2521 ccgagcagga cgaaaagacc ctgatcttct gcgccagcga cgcgcatgcc gacttggtgg
     2581 tggttctgct caaagaagcg ctggcgaaaa agtacggagc tgtcgacaac gatgcggtct
     2641 tgaagatcac cggccagacc gacaggccac tcgacgccat ccgtcgctat cgcaatgaga
     2701 ggcagcccag catcgtggtc acggtcgacc ttctgacgac gggcattgat gttcccaaga
     2761 ttgccaacct ggtgtttctg cgtcgggtca ggagccggat tctgtacgag cagatgattg
     2821 gccgggcgac gcgcctgtgt cccgaaattg gcaaggagcg ctttcgcatc ttcgacgcgg
     2881 tggacctgta ttcggcgctc aaagacgtga ccgatatgaa gccggtggtc gtcaatccgc
     2941 tggtttcgtt tgcgcagctc gcccaggaag tccgcgaagg gaaggaagag gcgccgcggc
     3001 gtgcgtccat cgacgagttt gtcgccaagc tccagcgcaa aaagcggatg cttttgcggg
     3061 atgcggaaga acagctcgcg acggcggcag gcatgggact gcgcgacctc gtgcagtttt
     3121 tgaaatcggc ccgcgtcgct gaggtggcgg actacctgac agcgcatccg gcgctggcgg
     3181 catttttgga tcgtgcgacg ggcagcggcg caaacaaagt catcgtgtcc gaaaagtccg
     3241 acgccatcca cgaagtcagc cgcggctatg gccccggcgt aagcggacga cccggcgact
     3301 acctggaatc gttccgcgcg tttgtatccg gcagtctgaa ccggcttccg gcgctgctcg
     3361 tcgtcaccca gcggcccaaa
//
LOCUS       DAORGN010000325         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_16805_length_3348_cov_6.081385, whole genome shotgun sequence.
ACCESSION   DAORGN010000325 DAORGN010000000
VERSION     DAORGN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16805_length_3348_cov_6.081385"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3275)
                     /locus_tag="PKL17_21125"
     CDS             complement(<1..3275)
                     /locus_tag="PKL17_21125"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNK47299.1"
                     /translation="MKPTLPKWPGSAPGEKTYWRSTDHAENSPLFRESLNRELPSEVP
                     PEVLNPLSRRSFMGILGASMALAGLSGCRRPEEKILPYTRTPEDLAIGLPTYYATAMP
                     LGSAVYGLLVESHEGRPTKIEGNPNHPMSLGGTNTHTQGAILELYDPDRSAGPLLAGK
                     AEKAPAAAAEAGEHGGGHGGEAPKPVLFGLDAVTDYFKTRRGKWAADKGRGVHVLSGA
                     LTSPTLLRLRDQLSKEWTDAKWWTWEPVCEDSIRQGSRTAFGEDLVTLNEVHKASVIL
                     SIGSDFVAHTPDGVRNARHFAAKRKVDRPVDDMNRLYVAESAWTVTGTNADHRLRLKP
                     SLLAHFALALALELQKRNLALAPEVVAQLPKAAPAGIDAKFVSAVCEDLLKAQGKALI
                     VVGKSLPAGVHALVHAIHAALGAVGETVNYVKSGDAGRALDRDAIKGLSAALDAGQVE
                     TLIVLGGNPVYDAPADLGLAEKLKKVQVVHLGLYRDETALVSALHIPRAHFLESWGDL
                     RAADGTASIVQPLIAPLYKGWSDIELVNYLLTGNMVRGYELVRDTWRGVAAAKFAPPP
                     PPPAPVEAAPVAPAGKGPAGKPVKPVPGAKPVVGVKPAGAAAVVAGAAAPAAPGAAPA
                     APGAAPADAAAPPPAVPQETLAVFFDRAFRKSLHDGVIEGTAFDKVRPQLKAADIGQA
                     VAQVSAIAPKAWEIEFLISNSTFDGRFANVSWLQELPDPITKIVWDNALLISVEGAKE
                     LGVERNDIVSLTVRGQTVEAAVFVVPGQSAGTVALALGYGHNADAVGKVGRGAGFNAY
                     KIRHSDGLWSDEVQVQKTGKKYEDPRDYWFGDTTHLTGLVTTQDHFAMEGRPLVQEAT
                     LGKFRKHPNFAQHAVHHPPLLALWDPYKRSGQQWGLVIDLSACVGCGTCTIACQAENN
                     VPVVGKPQVRKGREMHWIRIDRYFTFEPVAFDNVNRKKIELGDDNAQMIHQPVPCMQC
                     EDAPCENVCPVAATTHTQDGLNDMVYNRCVGTRYCANNCPWKVRRFNYLDYRGDVPEV
                     AKLAMNPDVTVRSRGVIEKCTYCVQRIRGAQRSAKLKDTQGRSEYVADGTIVTACQQA
                     CPA"
BASE COUNT          602 a         1108 c         1031 g          607 t
ORIGIN      
        1 tccgccgggc aggcctgctg gcaagccgtc acgatggttc catcggccac gtattccgac
       61 cgaccctggg tgtccttgag cttggccgac cgctgcgcgc cacggatgcg ctgcacgcag
      121 taggtgcact tttcgatgac gccgcgtgag cgcacggtca cgtcggggtt catcgcgagc
      181 ttggccactt cgggcacatc gccgcggtag tcgaggtagt tgaaccgacg aactttccac
      241 ggacagttgt tggcgcagta gcgggtgccg acgcagcggt tgtacaccat gtcgttgagt
      301 ccgtcctgcg tatgcgtcgt cgccgcaacc gggcagacgt tttcgcacgg ggcgtcctca
      361 cactgcatgc acggcaccgg ctgatggatc atctgcgcgt tgtcgtcacc cagttcgatc
      421 ttcttgcggt tcacgttgtc gaacgcgacc ggctcgaacg tgaagtagcg gtcgatgcga
      481 atccagtgca tctcgcggcc cttgcggacc tgcggcttgc cgacgaccgg gacgttgttt
      541 tccgcctggc aagcgatggt gcaggtgccg cagccaacgc aagccgacag gtcaatgacc
      601 aggccccact gctgacccga gcgcttgtag ggatcccaca gcgcgagcag cggcggatgg
      661 tgcaccgcat gctgggcaaa gttcggatgc ttgcggaact tgccgagcgt cgcttcttga
      721 acgagcggac ggccttccat cgcgaagtgg tcctgcgtcg taaccaggcc cgtcaggtgc
      781 gtggtgtcac cgaaccagta atcgcgcgga tcttcgtatt ttttgccggt cttttgaacc
      841 tggacttcgt ccgaccacag accgtcgctg tgacgaatct tgtacgcatt gaagccagcg
      901 ccgcgaccga ctttgccgac tgcatcagcg ttgtggccgt agcccagcgc gagcgccacc
      961 gtgcccgccg actgtcccgg taccacgaag accgccgctt cgacggtctg gccgcgcaca
     1021 gtcagcgaaa cgatgtcatt gcgctcgaca ccaagctctt tggcgccctc gaccgaaatg
     1081 agcagcgcgt tgtcccacac gatcttggtg attgggtcgg gcagctcttg cagccagctc
     1141 acgttggcaa agcggccatc gaacgtcgag ttcgagatca ggaactcaat ttcccaggcc
     1201 ttcggcgcaa tcgcagaaac ctgtgccacc gcctggccga tgtctgctgc tttgagctgc
     1261 ggacgcactt tgtcgaaagc cgtgccttcg atcacaccgt cgtgaagcga cttgcggaac
     1321 gcgcgatcaa aaaagaccgc gagcgtctct tgcggaaccg cgggcggagg cgcagcagca
     1381 tcggcaggag ccgcacccgg tgctgcagga gccgcgcccg gtgctgcagg agcagcagcg
     1441 cccgcgacca ccgccgcagc gcctgcgggc ttgacgccca cgacaggctt ggcacccgga
     1501 accggcttga cgggcttgcc cgccggaccc ttgccagccg gagccacggg ggccgcttcc
     1561 accggagcag gaggaggcgg cggcggtgca aactttgcag cagccacgcc acgccaggtg
     1621 tcccgcacca gctcgtagcc gcggaccatg ttgccggtca gcagataatt gacaagctcg
     1681 atgtccgacc agcccttgta gagcggcgcg atcagcggct gcacaatgct cgccgtaccg
     1741 tcggcggccc gcagatcgcc ccacgactcc aggaagtgag cgcgcggaat gtgcagcgcc
     1801 gaaaccagcg ccgtctcatc gcgatacagg cccaggtgaa cgacctgcac cttcttgagc
     1861 ttttcggcaa ggcccagatc ggcgggtgcg tcgtaaaccg ggtttccacc gaggacaatc
     1921 agcgtctcga cctgacccgc atcgagcgct gccgacagcc cctttatcgc atcacgatcg
     1981 agcgcgcgac cggcatcgcc cgacttgacg tagttgaccg tttcgccaac cgcaccgagc
     2041 gccgcgtgaa tcgcatgaac cagcgcatgc acgccagcgg gcagcgactt gccaaccacg
     2101 atgagcgctt tgccttgcgc tttgagcaga tcctcacaaa ccgccgaaac gaacttggca
     2161 tcaatcccgg ctggcgccgc tttgggcagc tgcgccacga cttccggtgc caacgccagg
     2221 ttgcgtttct gcagttcaag cgcgagcgcg agcgcaaagt gcgcaagcag gctcggcttc
     2281 agccgcaggc gatgatcggc attggttccc gtcaccgtcc acgccgactc ggccacatag
     2341 agccggttca tgtcatcgac tggacgatcg accttgcgct tggcggcgaa gtgtcgcgcg
     2401 ttgcgaacac cgtcgggcgt atgcgccaca aagtccgaac caatcgacag gatcaccgac
     2461 gccttatgca cttcgttcag cgtcacaaga tcctcgccga acgcggtgcg gctgccctga
     2521 cggatcgagt cttcgcagac cggctcccag gtccaccact tggcatcggt ccattccttc
     2581 gagagctgat cgcgaagccg aagcagcgtc ggcgacgtca gcgcacccga aagaacgtga
     2641 acgccgcggc ccttgtcagc cgcccacttg ccacgtcggg tcttgaagta gtcggtgacc
     2701 gcatccaggc cgaacagcac cggcttgggc gcttcgccac cgtgaccacc gccgtgctcg
     2761 cccgcttccg ctgctgcagc cggggccttt tcagccttgc cagccaagag cggtcccgcc
     2821 gagcgatccg gatcgtacag ttccagaatc gcgccttggg tgtgggtgtt tgtaccgccc
     2881 aggctcatcg ggtgattcgg attgccttcg atcttggtcg ggcggccttc gtgactttcg
     2941 acgagcagac cgtacaccgc cgaaccgagc ggcatcgccg tggcgtagta agtcggcaga
     3001 ccgatcgcca ggtcttcggg cgtccgcgta tacggaagaa tcttttcttc gggacggcgg
     3061 cagcccgaca ggcccgccag cgccatcgaa gcacccaaaa tgcccataaa cgaccgccgc
     3121 gacagcgggt ttaggacctc gggcggaact tcagacggca gctcacgatt gagcgactcg
     3181 cggaacagcg gactgttttc tgcgtggtcc gtgctgcgcc agtaggtttt ctcgcctggc
     3241 gcgctgccag gccacttggg aagtgttggc ttcatcggtg acacctagag caatcggtag
     3301 gtggcgcaat cttgagttcc tgggccagct tctccccgaa ctcgcgac
//
LOCUS       DAORGN010000326         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_16968_length_3328_cov_4.728995, whole genome shotgun sequence.
ACCESSION   DAORGN010000326 DAORGN010000000
VERSION     DAORGN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16968_length_3328_cov_4.728995"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PKL17_21130"
     CDS             complement(<1..942)
                     /locus_tag="PKL17_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47300.1"
                     /translation="MRTWKLLGTLGILTTAAVVACNNTTIGGDNCTDPNNCVQMCTDP
                     NGCNPQPGPEYTAINISPSPVTIQANGQPATKQLTATGTKTDGTMSSQLIGVNWSAPV
                     NPIGTIDASGVFTANGNVGGTVTVTATYVTNGVTLTSTVQITVNVNKVVTTPNTDPNA
                     PTLFMQTPTMDTTKAPAILYPLDGVMMPQNVYPADIQWSLVGTMGDIYRVTITKTNIT
                     TVAYVGYNTTTFNFDYLVDSKAWASIAQSNLDEDAVLTVDRYEKATGKVWSGTPVKMH
                     FANGSLLGTVYYWDINVGRIRKIQDGTATATDLIPTAP"
     gene            complement(1136..2983)
                     /locus_tag="PKL17_21135"
     CDS             complement(1136..2983)
                     /locus_tag="PKL17_21135"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47301.1"
                     /translation="MKTLTTASLCAFALAFGAVAPAFAQAQPRQGFELNRYQPTAAGE
                     WSFAVDHPWYSSTRYFAAGVTLNYAHQPLVTGTQDQNGVFSQTQSLIDHHFIAHIDLA
                     GSFLDRVLVTASLPIVLASSGDPAFAMGASVGDPRIGAKVRLYGQPYQDVFSASFGAD
                     LWIPLRAMTDSISPTSSDQTVRALPKLMLGGVWRKLLWSTTLGFLIRPEAVTVAPNGY
                     ASSLGRTASEIQLGLSAAYYDEDRRFAVGPEAILATSATGADSFSRFGTSLELLLAGH
                     YNIAHMVQAGLALGAGFVRQPGTPDFRALFRLAYAPMVDRKHDRDGDGILDADDACPD
                     VKGIRTGDPTTHGCPPPPDRDKDGVPDAVDLCPDVPKGSKPDPQRLGCPYVEPERDRD
                     GDGILDRDDLCPDLHKGELPDPARLGCPAPDTDMDGIQDPRDLCPTEHAGHYPDPQRL
                     GCPLPDRDKDTVPDKFDACPDKPGAPHPDPKKNGCPGLLEVRGGQIVILKPVFFATDK
                     DVILPESFPVLQAVAHALQAAPGIRLIRIDGHTDSQGNRLYNIDLSQRRTRSVRNWLI
                     QHGIEESRLAAEGYGPDRPIATNKTVEGRAKNRRVEFHIVDNGGVTTAQ"
     gene            complement(2996..>3328)
                     /locus_tag="PKL17_21140"
     CDS             complement(2996..>3328)
                     /locus_tag="PKL17_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47302.1"
                     /translation="QQDRLVYCTHQGPLAAGEQTHAVDIAVHNPNPVAADNEVTAQVI
                     SDGALDPNPADNFWAEYGANRRIAGGGVGCSLSTGHSSASTGALCALLFGMAWVLRRR
                     SVRVSEKQ"
BASE COUNT          598 a          990 c         1082 g          658 t
ORIGIN      
        1 tggggcagtg ggaatcaggt cggttgccgt cgctgtaccg tcctgaatct tgcgaatccg
       61 accgacgttg atgtcccagt aatagacggt gccgagcagc gagccgttgg cgaagtgcat
      121 cttcaccggg gtgccgctcc acactttgcc ggtcgctttt tcgtagcgat cgaccgtcag
      181 caccgcatcc tcgtcgaggt tcgactgcgc gatgcttgcc cacgccttgc tgtcgaccag
      241 atagtcgaag ttaaacgtgg tggtgttata gcccacgtat gcgacggtgg tgatgttcgt
      301 cttggtgatg gtgacgcggt agatgtcacc catcgtaccg acgagagacc actggatgtc
      361 ggcggggtag acgttctgcg gcatcatcac gccgtcgagc ggatagagga tggccggcgc
      421 tttggtcgtg tccatcgtcg gcgtctgcat aaacagcgtc ggcgcgtttg gatcggtgtt
      481 cggggtggtg accaccttgt ttacgttgac ggtgatctga accgtgctgg tcagcgtcac
      541 gccgtttgtc acatacgtcg cagtcaccgt cacggtgccg ccgacattgc cattggcggt
      601 aaacacaccc gatgcgtcga tggtgccgat cgggttgacc ggtgccgacc agttgacgcc
      661 gataagctgg ctgctcatcg tgccatcggt cttggttccc gtcgccgtga gttgcttggt
      721 ggcgggttgg ccgttggcct ggatggtgac cgggctcggc gagatgttga tcgcggtgta
      781 ttccggtccc ggctgcggat tgcagccatt gggatcagtg cacatttgga cgcagttgtt
      841 cggatcggta cagttgtctc cgccgatggt ggtgttgttg caagccacca cggcggcggt
      901 cgtcagaatg ccgagtgtgc caagaagctt ccaggttctc atgtagtgaa ctcccgtctc
      961 atcaagtgaa gcattgcggc tctagtgcga ggactcggcc attttaccgt cggagggggg
     1021 atcttggcaa acgtcgtcgg accgtcgata tgtgacaact tggcttcacc caccaacgtg
     1081 gggaagcctg ctgacaaacg gttttgagcg ggagaaagga agcggggcgg ggagcttact
     1141 gcgcggtcgt cacgccgccg ttgtccacaa tgtggaactc aacgcggcgg tttttggcac
     1201 gaccttccac ggtcttgttg gtcgcgatcg gacggtcggg accgtagccc tcggcggcca
     1261 gacgcgattc ttcgatgccg tgctggataa gccagttgcg cacgctgcgc gtccgtcgct
     1321 gcgacaggtc gatgttgtac agccggttgc cctggctatc ggtgtgtcca tcgatccgga
     1381 tgagacggat gcctggggcc gcttgcagtg cgtgggcaac cgcctgaagc accgggaagc
     1441 tctccggcag gatcacgtct ttgtccgtcg cgaagaacac cggcttcagg atcacgatct
     1501 ggccaccgcg gacttcgagc agacccggac aaccgttttt cttcggatcg ggatgcggcg
     1561 caccgggctt gtcggggcaa gcgtcgaact tgtccggcac ggtgtctttg tcgcggtccg
     1621 gcagcggaca accaagtcgc tgcggatcgg gatagtgacc cgcatgctcc gtcgggcata
     1681 gatcccgcgg atcctggatg ccgtccatgt cggtatccgg ggccgggcag ccgagccgtg
     1741 ctggatcggg cagctcgccc ttgtgcagat ccggacacag atcatcgcga tccaagatgc
     1801 catcgccatc gcggtcacgc tcaggctcga catacggaca accgagccgc tgcggatcgg
     1861 gcttcgatcc cttgggcaca tctgggcaca gatcgactgc atcgggcacg ccgtctttgt
     1921 cgcgatcggg cggcggtggg cagccgtgcg tcgtcggatc gccggtgcga atgcctttta
     1981 cgtccgggca cgcatcgtcg gcatcgagaa tgccgtcgcc gtcgcgatca tgcttgcggt
     2041 cgaccatcgg cgcatacgcg agccggaaca gcgcgcggaa gtccggcgta cccggctgac
     2101 ggacaaagcc cgcaccgagc gccagacccg cctgcaccat gtgcgcgatg ttgtagtgac
     2161 cggcgagcag cagctccaag ctggtgccaa agcgcgaaaa agagtccgct ccggttgccg
     2221 aggtggccaa aatggcttcg ggaccgaccg caaagcgacg atcttcatcg tagtaagcag
     2281 ccgacagacc gagctggatt tccgacgcgg tgcgacccag gctcgatgcg tagccgtttg
     2341 gcgcgacggt gacggcttcc gggcgaatca ggaagccaag tgtcgtcgac caaagcagct
     2401 tccgccacac accaccgagc atcagtttgg gcagtgcacg caccgtctga tcgctcgagg
     2461 tcgggctgat gctatcggtc atcgcgcgga gcggaatcca cagatccgca ccaaagctgg
     2521 ccgaaaagac atcctggtag ggctgtccgt agagccgaac tttggcgcca atccgtggat
     2581 caccgaccga tgcgcccatc gcaaacgccg ggtcgccact gctcgcgagc acaatcggca
     2641 gcgacgccgt gacaagcacg cgatccagga acgaaccggc caggtcgatg tgggcgatga
     2701 agtggtgatc aatcagcgat tgggtctgac tgaacacccc attttgatcc tgcgtgccgg
     2761 tgacgagcgg ctggtgcgca tagtttagcg tcacgcccgc cgcaaagtac cgcgtcgagg
     2821 aataccacgg gtggtcaacg gcaaacgacc actcgcctgc cgccgttggc tgatagcggt
     2881 tgagctcgaa gccctgccta ggctgggctt gcgcaaacgc gggcgcgacg gcaccgaacg
     2941 ccagtgcaaa agcacacaga gaagctgtag tcagcgtttt catggcgaat gctccttact
     3001 gcttctcgct tacccggacg ctgcggcggc gcagcaccca agccattccg aacagcagcg
     3061 cacacagcgc gcccgtgctt gccgagctgt ggcctgtgct caacgaacaa ccgacgccac
     3121 caccggcaat gcggcggttg gccccgtatt ctgcccagaa gttgtcggcg gggttcggat
     3181 caagcgcgcc gtcggaaatg acctgcgccg tcacctcatt gtccgctgcg accgggtttg
     3241 gattgtggac ggcgatgtcc actgcgtgcg tctgttcacc agcggcgagc ggaccttggt
     3301 gcgtgcagta caccagtcga tcctgctg
//
LOCUS       DAORGN010000327         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_17839_length_3241_cov_5.262084, whole genome shotgun sequence.
ACCESSION   DAORGN010000327 DAORGN010000000
VERSION     DAORGN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17839_length_3241_cov_5.262084"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKL17_21145"
     CDS             complement(<1..286)
                     /locus_tag="PKL17_21145"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNK47303.1"
                     /translation="MLLAVDIGNTNIVFGVFDGATLLTHFRIETRKARTEDEYAALLH
                     SMFTLHGLPFRAGPVRDSQPDDARAGEPSSASNPERTSAVSAQSSERGITA"
     gene            complement(350..1039)
                     /locus_tag="PKL17_21150"
     CDS             complement(350..1039)
                     /locus_tag="PKL17_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47304.1"
                     /translation="MKDIRDEIERLTALLSFRIEHLAKREGRAIHFSSYSVAVRPGQL
                     AQLFSTLVHLPRGYAATFMPRSQPESDGTWRIDVRRNDGVMRESYFDGIVAGPNGTEL
                     TLLFKGRPLQIEDLERILSDLGKPGLSGMSIDINRLYRMFFGKMDQGVTDQLGAIRDV
                     DLLDDIYTALMSGRSQGFVDQLLKQNTSTAPLASAATPPPAAPVAKEAVTESAPAVAQ
                     DPVQAPGDENS"
     gene            1201..2637
                     /gene="gndA"
                     /locus_tag="PKL17_21155"
     CDS             1201..2637
                     /gene="gndA"
                     /locus_tag="PKL17_21155"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HNK47305.1"
                     /translation="MSETQYEVGLVGLAVMGENLALNMLEKGFSVAVHNRTADRIDAF
                     LARASAEGFARTHGAVSAQAFVASLKRPRRIIMMVKAGQPVDDTIAKLRPWLEPGDML
                     IDGGNEHFTNTERRFRELSPSGIHYLGMGVSGGEEGARHGPSLMPSGPKEAFDSLAEV
                     VTRIAAQVEGPGGGPCVTYIGPGGSGHYVKMVHNGIEYGDMQLIAESYDVLRRVGGLS
                     NSELSDVFSAWNESELESFLIEITARIFKTPDPDGAGELIDKVLDSSGMKGTGKWTVL
                     DAVELGVPIPVIASAVDARVLSSQKAARVAASQKLSGPNPRPLSQTERAQLVDDVRAA
                     LYASKACSYAQGLRLLQVASQTRNWNLPLGEIAAIWRGGCIIRAQFLSRIKAAFDRDP
                     QLPNLLLDPSFASELLTRQAAWRRVVGLSAEHGVPTLAMSGALSYYDTIRQERLPACL
                     IQCQRDLFGAHTYERIDKPGTFHTDWAK"
     gene            2740..3111
                     /locus_tag="PKL17_21160"
     CDS             2740..3111
                     /locus_tag="PKL17_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47306.1"
                     /translation="MTKQIALLLLGLAGCASSNAAVSEDDALRERTFQFSGGDDLAVR
                     AAYDLRCTKPQMQCQVLQRAGMFQVAVTAGVRGCGYQATYVRGAGTSGAWILNGPVMP
                     DLPPPPPPMMAPPPAMAPAVR"
BASE COUNT          599 a          912 c         1064 g          666 t
ORIGIN      
        1 cggcggtgat gcctcgttcg ctcgattggg ccgagacggc gctggtgcgc tcggggttgc
       61 ttgctgacga gggttctccg gcgcgggcgt cgtctggctg cgaatcgcgc acaggaccgg
      121 cgcggaacgg cagaccatgc agggtgaaca tcgagtgaag cagcgccgca tactcgtctt
      181 cggtgcgagc cttgcgcgtc tcgatgcgaa aatgggtcag aagggtggcg ccgtcgaaga
      241 cgccaaagac aatgttggtg ttgccgatgt ccaccgcaag cagcatgcgg ctagcttaat
      301 ccaaagcgcg gctcgtcaac accggcggcc ccgcgcttcc gcgattttgc tagctgtttt
      361 cgtcgccagg ggcttggacc ggatcttggg cgaccgcggg ggcgctttcc gtgacggcct
      421 ctttcgcgac cggggctgcc gggggcggtg tcgctgcgct ggcgagtggt gccgtcgacg
      481 tgttttgctt gagcagctga tcgacaaagc cctggctgcg tccgctcatc agcgccgtat
      541 agatgtcgtc gagcagatcg acatcgcgaa tcgcgccgag ctgatccgtg accccttgat
      601 ccatcttgcc gaagaacatg cggtacagac gattgatgtc tatcgacatg cccgacagac
      661 ccggcttgcc gaggtcgctc aggattcgct cgaggtcttc gatctggagc ggacggcctt
      721 tgaaaagcag cgtcagctct gtgccgtttg ggcctgcgac gatgccgtcg aaatagcttt
      781 cacgcatcac gccgtcgttg cgtcgcacat cgattcgcca tgtgccgtcg gattcgggct
      841 gcgagcgagg cataaacgtc gctgcatagc cgcggggcag gtgaaccagc gtcgaaaaga
      901 gctgggcaag ctggccggga cggaccgcca ccgagtaact ggaaaaatgg atggcgcgtc
      961 cttcacgttt ggccagatgt tcgatacgaa acgaaagcag cgcagtgaga cgctcgatct
     1021 cgtcacgaat gtctttcatc gtggtgagat ggtaacacgt tttccgttcg gaaaatcaca
     1081 tttgtcgtcg gacaatcctt cggttatttc gcggacttac agcgcaaatg gtggctgtgc
     1141 tgctggccca cttagcgtgc atgctatgtt gtgcaatcgc tgtggaagga gacgctgcgc
     1201 atgagtgaaa cccagtacga agttggcctg gtcggactcg cggtgatggg ggaaaacctc
     1261 gcgctcaata tgcttgaaaa aggattttcc gtcgcagtac acaaccgaac ggcagaccgg
     1321 atcgacgcat tccttgctcg cgcaagtgca gagggatttg cccgaacgca tggggcggtg
     1381 tcggcgcaag cgtttgtcgc atcactcaag cgtccgcgcc gcatcatcat gatggtaaaa
     1441 gccggacagc ccgtcgacga taccatcgca aagctgcgtc cttggctgga accgggggac
     1501 atgctgatag acggtggcaa cgaacacttt accaatacgg agcggcgttt ccgcgagctg
     1561 tctccgtcgg ggattcacta tctggggatg ggcgtttccg ggggggaaga aggcgcgcga
     1621 cacggtcctt cgctgatgcc ttcggggccc aaagaggcgt tcgattccct ggcggaagtg
     1681 gtgacgcgga ttgccgcgca agtcgagggt ccaggcggtg gaccgtgtgt gacgtacatc
     1741 ggtccgggcg gatcgggaca ctacgtcaag atggttcaca acggcatcga gtacggcgac
     1801 atgcagctta tcgccgaaag ctatgatgtc ttgcgccgcg tcggtgggct gtccaattcc
     1861 gagctttcgg acgtgttttc ggcgtggaat gaaagcgagc tggagtcgtt cctgatagag
     1921 atcacggcgc gcattttcaa gacccctgat cccgacggtg cgggtgagct catcgacaaa
     1981 gtgttggaca gttccggaat gaaaggaacc ggcaagtgga ccgtgctcga tgcggtcgaa
     2041 ctgggggttc cgattccggt gatcgcaagc gcggtcgatg cacgggtttt gtcgtcgcaa
     2101 aaagcggctc gtgtggcggc cagtcagaag ctgtccgggc cgaatccgcg accgctttcg
     2161 caaacggaac gcgcccagct tgtcgatgat gttcgcgcgg cgctgtatgc cagcaaagcg
     2221 tgttcgtatg cgcaaggtct gcggcttctg caagtcgctt cgcagacgcg aaactggaat
     2281 cttccgctcg gtgagattgc tgcgatttgg cgtggcggct gcatcattcg cgcgcagttt
     2341 ctgtcccgca tcaaagctgc gtttgatcgc gatccgcagc ttccgaacct gctgctcgat
     2401 ccgagctttg cttccgagct gctgacacgg caggcggcgt ggcgacgggt cgtaggactt
     2461 tcagcagaac atggagtccc gacgctggcg atgagcggcg cgctttccta ttacgatacg
     2521 attcgtcagg agcgtctgcc cgcatgcttg attcagtgtc agcgcgattt gttcggtgcg
     2581 cacacctacg aacggatcga caagccgggg acgtttcaca ccgattgggc gaaatagtcg
     2641 cttctcactt tccgccgcgc cgcagccagt gctaggttcc cccgcgccgg tgattccctc
     2701 aacaccggaa aaagacttcc ccaaatagga gacgggacca tgaccaaaca gatagcgctg
     2761 ttgctgctgg ggctcgccgg ctgtgcgtca tccaatgcag cggtttctga agacgatgcg
     2821 ctgcgcgagc ggacgtttca gtttagcggt ggcgacgatc tggcggtgcg ggcagcctat
     2881 gatctgcgct gtaccaagcc acagatgcag tgccaagtgc ttcagcgcgc cgggatgttt
     2941 caggtcgccg tgaccgcggg tgttcgtggc tgcggctacc aagcgacgta tgtgcggggc
     3001 gcgggaacgt cgggagcctg gattctcaat ggtccggtga tgccggatct gccgccgccg
     3061 cctccgccga tgatggcgcc gccgcctgcg atggctccgg cagtacgtta gcgatcacgc
     3121 tgctgcggca cacggattcg tgccagtcgc cgtggccgtg ggaatcgatc tgcaagtccc
     3181 agctcggcgg cgaaccggca accccggatg atccggcccg agggctcgtg tggtcggcga
     3241 t
//
LOCUS       DAORGN010000328         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_19132_length_3116_cov_5.267886, whole genome shotgun sequence.
ACCESSION   DAORGN010000328 DAORGN010000000
VERSION     DAORGN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19132_length_3116_cov_5.267886"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..952)
                     /locus_tag="PKL17_21165"
     CDS             complement(224..952)
                     /locus_tag="PKL17_21165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNK47307.1"
                     /translation="MLGIAFEGCACRAAFHAGVAAALSEAKLSFSLSAGASSGSLCAA
                     AVAAEKGTQLPTMWRELAGRNVISLRRLLWNRSPFDMSHIVRNALRNNLGSLDLRTFP
                     TEALIVATRLRKLDRIVYSSHQESDLIEPLMGSCFFPILYGRPVKVRNDWLLDGGAID
                     NMPVEILAERGVTDIVGVVTSHEGIVNKTPLRKRWRPSVHGAKLHIIKPSRPLAIKSW
                     DFDVDHMNQAIDEGYRQGRLFVGQ"
     gene            1098..2135
                     /locus_tag="PKL17_21170"
     CDS             1098..2135
                     /locus_tag="PKL17_21170"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNK47308.1"
                     /translation="MKVAAHVPGRSGSLWRLLAVTLLGGGLLTSLSCEHRAATAKPRP
                     KKEKAAQLATLKLFENAPTDEPPPQGFLLSGEPEHAKEDKPNITKLATSEVPCPKDPP
                     PGMVCIPGGPFFRGNDHGGKPDEKPRMRITVSPFFLDTTEVTNEQYFRCVEAGKCEPP
                     IKYFGLYVRPKQPVVAVSWYQAYDFCHYANKRLPTEAEWEKAARGPDGEAYPWGNEQP
                     NCKLAVYEEPLGAKGCGTGTTADVATHGAFRYGLYDMAGNSWEWVNDWKSDCYKGCKN
                     ECGTACDGPDPQGPCGGGPKYCKGYSEKLLKGGSWYFTGDRMRGAERRGVPPSNRGPH
                     RLGFRCAKTIE"
     gene            complement(2187..2729)
                     /locus_tag="PKL17_21175"
     CDS             complement(2187..2729)
                     /locus_tag="PKL17_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47309.1"
                     /translation="MLLQSLPLTLALCLGTAVGSARADDPPPSQRISVSYFGESFRNP
                     GARLGYEGSLWKTGPFEYLLGGSIGLFFEPGARRGFFGLVETGARLTTDIGLLFDLRI
                     GVGYLNIAWDETTQHPQPSLSTSHFMPSALLGIGYDFRRVSSAPVGILVRSGMIGRYS
                     TDDNFDGAYVIDAGLFFTLK"
     gene            2870..>3116
                     /locus_tag="PKL17_21180"
     CDS             2870..>3116
                     /locus_tag="PKL17_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47310.1"
                     /translation="MKVTKTAMPWLGCVTAGLLWAGQAAAQPLDQGVPSEGSELDLFK
                     LDSLVNAAVVTSSGGEEEDRATAAATIYTITRDEILQR"
BASE COUNT          710 a          885 c          923 g          598 t
ORIGIN      
        1 ctccctacaa attccgaaag gattcctaac tgattccgaa aggattccta actgattccc
       61 aatcgactcc caatcgactc ccaatcgact cccaatcgac tcccaatcga ctcccaattg
      121 tattcccaat cgtattccca acccacttcc aaacccaggc gagagggaaa aaagaagagg
      181 ccgtttcgga ttccgcacgg gtgggtttgg ggcgctgttg tgcttattgt ccgacgaaaa
      241 gtcgaccctg ccgatagcct tcgtcgatcg cttgattcat gtgatcgaca tcgaagtccc
      301 acgacttgat cgcaaggggt cggctcggtt tgatgatgtg cagcttggca ccgtggacag
      361 aaggacgcca gcgcttgcgc agcggggttt tgtttacgat tccttcatgc gaagtgacaa
      421 cccctacgat gtctgtgaca ccgcgttctg ccaggatttc gaccggcatg ttgtcgatgg
      481 cgccaccatc taacagccag tcattgcgga ccttgaccgg cctgccgtac agaatcggaa
      541 aaaaacaaga gcccatcagc ggttcaatca gatcgctttc ctgatggctg gaatagacaa
      601 ttctgtctag ttttcgcaga cgggtcgcaa cgattagtgc ttccgtcgga aatgtgcgca
      661 gatccaggct tcccaagttg tttcgcaggg cgtttcgcac gatgtgactc atgtcaaacg
      721 gcgatcgatt ccacagcaag cgtcgcagcg agatcacatt ccgtccggcc agttcgcgcc
      781 acatggtggg aagctgggtt cccttttcag cagcgacggc agcagcgcac agtgaaccgg
      841 acgatgcacc ggctgaaagg gaaaacgaga gcttggcttc tgatagtgca gcagcgacgc
      901 cggcatgaaa cgcggcccga catgcacatc cttcaaacgc gattccgagc atgactttgg
      961 gagtctaatc cgactttcgc ccaagcagtg cgcggacggc acccttgcga cgacaatcgc
     1021 gcaaaaaaat gggaagtcag aaggtcgggc attggggttg acgcagcggc tcgcaatcgg
     1081 ttttgataag ttccgagatg aaagtagctg ctcatgtgcc cggtcgctcg ggttcgctct
     1141 ggcgacttct ggcggtgaca ctgctcgggg ggggactttt gaccagcctg tcctgcgagc
     1201 atcgcgccgc aaccgccaag ccgcgtccga aaaaggaaaa agcggcacaa ctggcgacgc
     1261 tcaagctgtt tgaaaacgcg cccaccgacg aaccgccgcc gcagggattt ctgctttcgg
     1321 gcgagcctga gcatgcgaag gaagacaagc caaacatcac caagctggcg acgagcgagg
     1381 tgccttgtcc caaagatccg ccgccgggga tggtctgcat tccgggcggg ccgtttttcc
     1441 gcggcaacga tcatggcggc aagcccgacg aaaaaccgcg gatgcgaatc acggtgtcgc
     1501 catttttcct cgataccacc gaagtgacca acgaacagta ctttcgctgc gtcgaggccg
     1561 gcaagtgcga gccgccgatc aagtactttg gtctgtacgt tcggcccaag cagcctgtcg
     1621 tcgctgtaag ctggtatcaa gcctacgatt tttgtcacta cgcaaacaag cgcttgccga
     1681 cggaagcaga gtgggaaaaa gcggcgcgcg gtcccgacgg ggaagcgtat ccatggggca
     1741 acgagcagcc aaactgcaag ctcgcggtgt acgaagaacc gctgggcgcc aagggctgcg
     1801 gcaccggcac gacggcagat gtcgccacgc acggcgcgtt ccgttacggc ctgtacgaca
     1861 tggccggcaa cagctgggag tgggtgaacg actggaaaag cgactgctac aagggctgca
     1921 aaaacgaatg tggcacagcc tgcgacggtc ctgaccccca aggtccgtgc ggcggtggtc
     1981 ccaaatactg caagggctat tcggaaaagc tgctcaaggg cggttcgtgg tacttcaccg
     2041 gcgatcgcat gcgcggggcc gaacgtcgtg gcgttccccc gtcgaaccgc ggcccgcacc
     2101 ggctgggctt ccgctgcgcc aagacgatcg aataacgccg cgtcgcgagc ccacttcggc
     2161 tcgtcgaaaa aaagcagaca accagcttac ttgagggtga aaaacagacc ggcgtcgatg
     2221 acatacgcgc cgtcgaagtt gtcgtctgtc gagtagcgac cgatcatgcc ggaacggacc
     2281 aaaatgccga cgggggcgct gcttacgcga cggaaatcgt agccgatgcc gagcagcgcc
     2341 gaaggcataa agtggctcgt cgacagcgac ggctgcgggt gctgcgtggt ttcgtcccac
     2401 gcgatgttca gatagccgac gccgatgcgc agatcgaaca ggaggccgat gtcggtggtc
     2461 agtcgggcac ccgtttcgac aaggccaaaa aaaccacgtc gcgcgccggg ttcaaagaaa
     2521 agcccgatgc tgccgccgag cagatattca aacgggccgg tcttccacag cgaaccttca
     2581 tagcccaggc gcgcgcccgg attgcggaac gattcgccaa aatagctgac agagatgcgc
     2641 tggctcggcg gtggatcgtc ggcgcgcgca cttccgacgg ctgttccgag gcagagcgcc
     2701 agggtaagcg gcagactttg caacagcaaa tcaagacaat tttttcttgt tgttaagtaa
     2761 ccaaaatatg ggccactttt ggttgctgtt tgcatcatcg cgctggcctg ttgtcgacgc
     2821 agtttgccat tgaaaaaagg ggcttcttta aaatagcctt taatatgcaa tgaaagtcac
     2881 aaaaacggca atgccttggc tgggctgcgt gactgctggg ctgctttggg caggtcaggc
     2941 tgcggctcag ccacttgatc agggcgtgcc gtcggaaggc agcgagctgg acctgtttaa
     3001 gctcgattcg ctggtcaatg ccgccgtcgt gacttcgtca ggtggagaag aagaagatcg
     3061 tgccacggca gcagcgacga tttacaccat cacccgtgac gagattttgc agcgcg
//
LOCUS       DAORGN010000329         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_19154_length_3114_cov_5.912390, whole genome shotgun sequence.
ACCESSION   DAORGN010000329 DAORGN010000000
VERSION     DAORGN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19154_length_3114_cov_5.912390"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PKL17_21185"
     CDS             complement(<1..1765)
                     /locus_tag="PKL17_21185"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNK47311.1"
                     /translation="MSIAATINSRLGRFLCGLWLLAPSLGLAAEDDAAETSKPRAQVD
                     VIGAVNLSRSTVWDSPRATSAVLRDELRVRPARMSGDTLQTEDGVVVQRPSYGTALPS
                     IRGLGDSRVLVVVDGIRLNTTTTSSLPSGLGNINLVDPYLLEAVEVVRGPGLASYGSD
                     GLGGTISLRTRRPVAIAGANLELSAGSRLSYASFDQSFSGSLSGGGRWGRFAAETAFS
                     ARRFNDLSGGSRAPSIPLSSFNDGGLYLGISADVGRGNVLLIYQGGRQYDGFRGERSQ
                     PDDLYLLTELARDLAYVRYTTSIEIADSSVDVTATAAYHRLSEQATRAQVAFDRVDRF
                     GNRDDVLALLANVRADLGRGGRFSAGLEGYFDWVASSAEHATVGGSGVNTASPEWLRY
                     PGGSAAHSFALFVIDELDLEHLFRGEPSSQPGRVKLLASGRVGGNFLIVGADDRLSRL
                     FPSLKMPQGRQLDQTWVYSGSLHLRYEFVPGAAFVAGFMLGTRPASLDDQARLDLGRP
                     GLLLPTETPLLQETSYSGEAGFRLAYPRIELAALYAVNSLVHPISLTTTALMATDCYT
                     LVAGRCVDRLLARRNEDSALLH"
     gene            complement(1781..2086)
                     /locus_tag="PKL17_21190"
     CDS             complement(1781..2086)
                     /locus_tag="PKL17_21190"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNK47312.1"
                     /translation="MTQTLTEDQLGTALRDPQLRRWAIQNGKLHRDYQFSSFVEAFAF
                     MTAVALHAEAMNHHPEWSNVYSRVSVSLQTHDAQGITERDIALARLIEKQAAGRTVG"
     gene            2200..>3114
                     /locus_tag="PKL17_21195"
     CDS             2200..>3114
                     /locus_tag="PKL17_21195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNK47313.1"
                     /translation="MLVIPVGGCGEFGRNLTAYFCAQTLVLVDCGIQMPDDLSPGIDH
                     IVPDLSALLARFGPPSAVVLTHGHEDHVGAVYHLLRTLGTPIPIYGRPLALRLCQNRL
                     RRNGVAMKLFDPRPLLPGEPLFLSSASEPDAQGLHVTPLAVPHSIPEACSILLRGPLR
                     PSQLIDGNPPSALPQNVRSVLHTGDFKVVADTVRFAPRPEPLPRIDLLVGDSTNSLSA
                     GSSGDERIVEEALFDVLKTRPGRVAVTLFSSHIERIGHFARSCQQLGRRLCLLGRGLH
                     DALDAAEAVGALRLPSSLLCTPDEAATLP"
BASE COUNT          604 a         1044 c          919 g          547 t
ORIGIN      
        1 agtgaagcag cgccgagtct tcgttgcgcc gcgcgagcag tctgtcgaca cagcgccccg
       61 ccacaagcgt atagcaatcg gtcgccatca gcgccgtcgt cgtcagtgag atcggatgca
      121 ccagcgagtt taccgcgtac agcgccgcca gctcgattcg cggataggcc agccgaaagc
      181 ccgcttcacc tgaatacgag gtctcttgca aaagaggcgt ttccgtcggc aaaagcaagc
      241 cgggacgacc cagatcaagc cgagcctgat catcgaggct ggcgggccgc gtcccgagca
      301 taaagccggc aacaaacgcg gcgccaggca caaactcgta gcgcagatgc agagagccgg
      361 aatagaccca cgtctgatcg agctgccgcc cctgtggcat tttcaaagat ggaaacagcc
      421 gcgacagtcg atcatccgca ccgacgataa ggaagttgcc tccgacgcgc cccgacgcca
      481 gcagcttgac ccgaccgggc tgactgctcg gctcgccgcg aaacaggtgt tccaagtcca
      541 gctcgtcgat gacaaacagc gcaaagctat gggccgccga tccaccggga tagcgcagcc
      601 attcggggct ggcggtattg acgccgctcc caccgacggt ggcatgttcg gcgctgctcg
      661 ccacccagtc aaaatagcct tccagacccg ccgaaaaccg tccgccgcgt ccaagatcgg
      721 cgcgcacgtt ggcaagcagc gccagcacat catcgcggtt gccaaagcgg tcgacccgat
      781 caaaagccac ctgggcgcgc gtcgcttgct cggacaagcg gtgatacgcc gcagtcgcgg
      841 tcacatcgac ggaagaatcg gcaatttcga tgctggtggt atagcggaca taggccagat
      901 cgcgcgccag ctcggtcagc agatacaggt cgtcaggctg gctgcgctca ccgcggaagc
      961 cgtcgtactg tctgccgccc tggtaaatca gcagcacgtt gccgcgtccg acgtcggcac
     1021 ttatccccag atacagtccg ccgtcattga atgacgaaag cggtatcgac ggagccctcg
     1081 atccgccact taagtcgttg aagcgacggg cggaaaaagc cgtctcggcg gcaaaacgtc
     1141 cccagcgacc cccgcccgac agactgcccg aaaaagactg gtcaaagctg gcgtagctga
     1201 gccggctgcc ggcgctgagc tccaagttgg caccggcaat cgcaaccggg cgacgggtcc
     1261 gcagcgaaat cgtgccgcca agtccgtcgc tgccatacga agccaggccc ggaccgcgca
     1321 ccacttcgac cgcttccaac aaatacgggt cgaccaggtt gatgttgcca agcccagacg
     1381 gcaagctgct cgtcgtcgtg gtattgagcc gaatcccatc gaccaccacc agcacgcgcg
     1441 aatcaccaag cccgcggatc gacggcagcg cagtgccata acttggccgc tgcaccacca
     1501 caccgtcttc ggtctgcagc gtatcgccgc tcatccgcgc aggacgcacc cgcagctcgt
     1561 ctcgcagcac tgccgacgtc gcccgcggac tatcccacac cgtgctgcgc gacaggttga
     1621 ccgccccgat gacatcgacc tgcgcccgcg gcttgctggt ctcggcggcg tcgtcttcgg
     1681 cggccagtcc caaagacggc gcaagcagcc acagcccaca aaggaaccgg cccagcctcg
     1741 aattgatggt tgctgcgatg ctcaaacaaa ctcccttggg ctagcccacc gtccggcccg
     1801 cagcctgctt ttcgataagc cgcgccagcg caatgtcccg ctcggtgatg ccttgtgcgt
     1861 cgtgcgtttg cagtgacacc gacacgcgcg aatacacatt actccactcg ggatgatgat
     1921 tcatcgcttc ggcgtgcagc gccactgcgg tcatgaacgc aaacgcctcc acaaacgacg
     1981 aaaactggta gtcgcggtgg agcttgccat tctgaatggc ccagcgccgc aactgcggat
     2041 cgcggagcgc cgtcccaagc tggtcttccg tgagagtctg cgtcatcgct ttggcgcatt
     2101 ttgagcacag gatgcggcca gccggcaagc gccaacacaa aacagacaaa aggtgcgcgt
     2161 ctttacggcc tccgcgcaca ctgctagtgt ccgcgcttca tgctggtgat tccggtcggt
     2221 ggctgcggtg agtttggccg caacttgacg gcctactttt gtgcccaaac gcttgtgctg
     2281 gtcgactgcg gcattcaaat gccggacgac ctgtcacccg gtattgacca tattgtcccc
     2341 gacttgtcgg cgctcttggc tcgctttggc ccgccgtcgg cggtggtgct gacccacggg
     2401 cacgaagacc acgtcggagc ggtctatcat ctgctacgca cactgggcac accgattccc
     2461 atttatggta ggccgctcgc gctgcggctg tgccaaaacc ggctgcgtcg caacggcgtc
     2521 gcgatgaagc tgttcgatcc gcgaccgctt ttgcccggtg aaccgctgtt tttgtcgtct
     2581 gcgagcgagc ccgatgccca gggactgcat gtgacgccgc ttgcggtgcc ccactcgatt
     2641 cccgaggcgt gctcgatttt gctgcgtgga ccgctgcggc catcgcagct tatcgatgga
     2701 aacccaccga gcgcgctgcc acaaaacgtc cgcagcgtgc tgcacaccgg cgacttcaaa
     2761 gtcgttgccg acacggtgcg ctttgcgccg cgccccgagc cgctgcctcg cattgatctg
     2821 ctcgtcggag acagcaccaa ctcgctttca gcgggctcgt cgggtgatga acgcatcgtc
     2881 gaagaagcgc tgttcgatgt gctgaaaacc cgccctggtc gcgttgcggt gacgctgttt
     2941 tcgtcgcaca tcgaacgcat tggccacttt gcccgcagct gccagcagct tggtcgacgg
     3001 ctgtgtcttt tgggacgagg gcttcacgat gcgctcgatg cagcggaagc ggtgggggca
     3061 ctgcggcttc cgtcgtcgct gctctgcaca ccggatgaag cggcgaccct gccc
//
LOCUS       DAORGN010000330         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_19828_length_3054_cov_6.331444, whole genome shotgun sequence.
ACCESSION   DAORGN010000330 DAORGN010000000
VERSION     DAORGN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19828_length_3054_cov_6.331444"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKL17_21200"
     CDS             complement(<1..1042)
                     /locus_tag="PKL17_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47314.1"
                     /translation="MRAARLLVAGLGLLWGIGTAQAQNVRFDVQNFRPTPGPRDLIIV
                     PQSQPLSHLSGAVGAYFSFSLDPLVLLNSDGQRAADVVKNRLELDVMAAIGLSNWLEV
                     GMVMPVVLFQESSNLEVIGEEGNIKKNAVGDLAALLKVPFVRRLSYASGFGVSLQGRV
                     NFPTGDQQAFASDGRVTFTPSFVADYRFGFGALLALQAGVHIRPAIEFMDIKLGPAFI
                     GAAGAELPIIRKYGITTLGGVYFNVPLQVLPESIRQIPAEAMLGLRWYASFGVTFTTG
                     FNFGADCGFSIPTFRYFLSAVWVPGKTREYEAIESFKKPPDDPDGDGLIAPVDHCPEE
                     KGPAENNGCPERD"
     gene            complement(1046..>3054)
                     /gene="traA"
                     /locus_tag="PKL17_21205"
     CDS             complement(1046..>3054)
                     /gene="traA"
                     /locus_tag="PKL17_21205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane exchange protein TraA family
                     protein"
                     /protein_id="HNK47315.1"
                     /translation="APNPRDSKGNIAPGTGLCAGYRFLGPPENQGGPSAGTYFKPRPS
                     SGFPAVGANDFPDSVNAFMDGQTGAATSGMRVDTTMLVPFDLTNFLDGISTNSKGDYL
                     ATRGCPTYMGKNYGCWFPGAPQDENVLAGSFGARFRGFINILPEWVNQTLHFGFLTDE
                     AVAMYLYTKAKPPQGPETKLVISRGLNPTSDGVYKFRVTNAVRFQKAGIYPIEVTYAQ
                     YSTSAALEMAVLFGSSFVDLDENAELPGTKPLGTWNFSLDYTQPTQFFQTASGVLPFD
                     GQPNRCKQCPRSLANIPNQPSGTCDPGMFCNEAAVCAPCIGDQFCGKSCKECLAPEPF
                     CIRDPRDPGGDYQCAECRDDMDCTAGRKCLQGKCVNPCNCCPGQFCIPTDPSKPDVRN
                     CSQCRTDTDCGAGKCDLLNGRCIDKIPDCNTDDRCGKSCVSCPDSTKNDPQGVRPFCL
                     NGEVCVQCRYDADCKVGTFCRSGDCVPCSDDRHCGPNCKTCGIDYVIASDGTISSKPP
                     TDKPFCYTPDNRVDTATCVRCRNDKDCGEGGTCDATTHQCSNTCATPCPPNQVCSGGK
                     CVECFTNAQCPCGICVDGTCTPNCESSSDCLGTQCCSKDTKTCISGRCKPGLTAHGGA
                     LCCSGTQVGSTDDPIAPGPNRGLWMGSLLTLLLLGWSTLRKVRS"
BASE COUNT          608 a          925 c          912 g          609 t
ORIGIN      
        1 tgtctcgctc ggggcagccg ttgttttccg ccggaccttt ttcttcggga cagtggtcga
       61 cgggagcaat cagtccgtcg ccatctggat cgtcgggcgg tttcttgaag ctctcgatcg
      121 cttcatactc gcgggtcttg cccggtaccc acaccgccga caaaaagtaa cggaacgtcg
      181 ggatgctaaa gccgcagtca gcgccgaagt taaagcccgt cgtgaacgtg acgccaaacg
      241 atgcatacca gcgcaggccc agcatcgctt cggcgggaat ctgtcgaatc gactcgggca
      301 gcacttgcag cggcacgttg aaatacacac cgcccagcgt cgtgatgccg tacttgcgaa
      361 tgatcggcag ttctgcgccc gctgcaccga tgaacgccgg accgagcttt atgtccatga
      421 actcgatcgc gggtcgaatg tggacgccgg cttgcagcgc caaaagcgcg ccaaaaccaa
      481 accgatagtc ggcaacaaag ctgggcgtaa acgtgactct gccgtcgctg gcaaacgctt
      541 gctggtcgcc tgttgggaag tttacccgac cttgcagcga cacgccgaag ccgctcgcat
      601 acgacagacg acgaacgaac ggcactttga gcagcgccgc caagtcaccg acggcatttt
      661 tcttgatatt gccttcctcg ccgatgacct ccagattgct cgactcttgg aacagcacca
      721 ccggcatcac catgccaact tccagccagt ttgacagacc gatcgccgcc atcacgtcga
      781 gttcgagccg atttttgacc acatcggcgg cgcgctgtcc gtcgctgttc agcagcacga
      841 gcggatcgag cgaaaagctg aagtaggccc caaccgctcc cgacagatgg gacagcggct
      901 gcgactgcgg aacaatgatc aggtcgcgcg gacccggcgt cggacgaaag ttctggacgt
      961 cgaagcggac attttgcgcc tgggccgtac caatgcccca caaaaggccg agcccagcga
     1021 cgagaagacg agcggcgcgc atgggttagc tccgcacttt ccgcagcgtc gaccagccga
     1081 gcaagagcag cgtcaaaagc gaccccatcc acagaccccg attggggccg ggcgcgatcg
     1141 gatcgtctgt cgagcccact tgtgttccgc tacagcacag cgccccgccg tgtgccgtaa
     1201 gtcccggctt gcaccgtcca gaaatgcacg tcttggtgtc tttcgagcag cactgggtgc
     1261 ccaaacaatc ggagctactc tcgcagtttg gtgtgcaggt tccgtcgacg cagatgccgc
     1321 acggacactg cgcgttggtg aagcattcga cacatttgcc gccgctgcac acttggtttg
     1381 gcgggcatgg cgtcgcgcag gtgttgctgc actgatgggt tgtcgcgtcg caggtgccgc
     1441 cctcgccaca gtctttgtcg ttgcggcagc gcacacacgt cgcggtatca acgcggttat
     1501 ccggggtgta acaaaatggc ttgtccgtcg gtggcttgct cgaaatggtg ccgtcggacg
     1561 cgatcacgta atcgatgcca cacgtcttgc agttgggccc acagtgacgg tcatcggagc
     1621 agggcacgca gtcaccactg cgacagaacg tcccgacttt gcagtcggcg tcgtagcggc
     1681 actggacgca gacctcgccg tttaggcaga acggtcgaac gccctgcggg tcatttttgg
     1741 tgctgtcggg gcagctgacg cagcttttgc cgcagcgatc gtcggtgttg cagtccggaa
     1801 tcttgtcgat acagcgcccg tttagcaggt cgcacttgcc cgcgccacag tcggtgtcgg
     1861 tgcggcactg cgaacagttg cggacatcgg gcttgctggg gtccgtcggg atgcaaaact
     1921 gacccgggca gcagttgcac ggattgacgc attttccttg gagacacttg cgaccagcgg
     1981 tgcagtccat gtcatcgcgg cactcggcgc actggtagtc accaccgggg tcgcgcggat
     2041 cgcggatgca gaatggctcg ggtgccagac actctttgca cgacttgccg cagaactggt
     2101 cgccaatgca cggcgcgcag acggcggcct cgttacagaa catgcccgga tcacacgttc
     2161 ctgacggttg attcggtatg ttggcgagac tgcgcgggca ctgcttgcag cgattcggct
     2221 gcccgtcaaa cggcagcacc ccactcgccg tctggaaaaa ctgcgtcggc tgcgtgtaat
     2281 cgagcgaaaa attccaggtc ccgagcggct ttgtacctgg caattccgcg ttttcgtcga
     2341 gatcgacaaa gctcgatccg aacaaaacag ccatctctag cgcagcagac gttgagtact
     2401 gcgcgtaggt gacttcaatc ggatagatgc cagccttctg aaatcgaacc gcattggtca
     2461 cgcggaattt ataaacacca tcgctggtcg ggttgagacc gcgacttatc acaagcttgg
     2521 tctcgggccc ctgtggtggt tttgccttgg tatacaagta catcgccacc gcttcgtccg
     2581 tcagaaagcc aaaatgaagc gtctggttga cccactctgg gaggatgttg ataaacccgc
     2641 gaaaccgggc gccaaacgat ccagccagca cgttttcgtc ttgtggcgca ccagggaacc
     2701 agcagccgta gtttttcccc atgtaggtgg ggcagcccct cgtcgccagg tagtcgcctt
     2761 tgctgtttgt gctgatgcca tcaagaaagt tcgtcaggtc gaaaggcacc agcatggtcg
     2821 tgtccacgcg catgccgctc gttgctgccc cggtctgacc atccatgaaa gcattcacgg
     2881 aatcaggaaa gtcgttcgcc cccaccgccg ggaagcccga actgggtcgt ggtttaaaat
     2941 aggtgccagc cgagggtccg ccttggtttt ccggtggccc gaggaaacga tatccggcac
     3001 aaagtccggt gccgggggcg atgttgcctt tgctgtcgcg cggattgggc gcca
//
LOCUS       DAORGN010000331         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_19830_length_3054_cov_4.977326, whole genome shotgun sequence.
ACCESSION   DAORGN010000331 DAORGN010000000
VERSION     DAORGN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19830_length_3054_cov_4.977326"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="PKL17_21210"
     CDS             <1..2632
                     /locus_tag="PKL17_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HNK47316.1"
                     /translation="LPDIVARRLLKPVSEEAAAELRAAFSQIVRMRDETLQTLLTTDA
                     ARGMFEKVYPFSPALLQTLVAVSSALQRTRTAIRLLLQLLVARRNDLALGEVVPVGDL
                     WDVVADGDEPFMDAMRLSFERARKLYTQKLRPLLESTHGVRWDDLRNLPTDDPKRTAF
                     RAEDRLLKTLLLAALVPEVESLRSLTANRLAALNYGSIQSTIPGREGRLILERIRSWS
                     AQVGEIRVSEDRDPVIQLQLSGVDTDSILERAKIQDSTAQRRQLIKRLLFARLGVLEQ
                     DSLYIEHEVIWKGTSRRVEVVYANVRELTDESLRSRGDEWRVLIDFPFDEENHGPADD
                     RAKVQSFLERGESAHTFVWLPRFFNRQTKQNLGTLVILDYLLTGERFVDHASHLSAVD
                     RAAARAILESQKSQLEGTIGQALLEAYGIAGNTDRPNFVESSHSIDQTFVSLEPALSL
                     TPPVAADLRAALEKIIGQLLSWQFPAHPDFEEEIKSSQIKKVCAEVLRATQVREGRII
                     VPKELRPLLRQIANPIKLGEMTDGDAFLLGQHWRQHFDRLYHQEETIKKGTVSGRRPL
                     TVRMLRSYIDLPRKMGLLPELQNLIIVVFAAQTNRAFFQHGGPVEGKLGELHDELELR
                     EQDLPDEADWVQARHRAESVFGLTGVSELRNAVNVAELAQKLRDKVQAAREDANLLLP
                     RLAEYYQALGIVEPAACRLQTARTALTLLESMTSGDAKDVIRALGKASLLPTPQAIGA
                     SVSQAKVVGAALGESRLQVICGTLHLSDSRREAACAMLGTLKDALVRDELALPLAPVL
                     SASERTAVSLLAPKATERPSLSPAKAAAQGSRSNVVAEGSRSGLSGEAIGAVLQEIAK
                     ASQGKGRRLSVSWSVEEDKE"
     gene            2629..>3054
                     /locus_tag="PKL17_21215"
     CDS             2629..>3054
                     /locus_tag="PKL17_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47317.1"
                     /translation="MSSGTLSESLLRAKVEEVRRKVPQAMAIGIKVDAPWRGCEQLTL
                     GDAAMRVVYCPSVLAFREQLAQAETEKLVLLTNQKEQELGSDVLYRLALSRLHEVRPW
                     QVILERFQARRWDSRLTKEPWMEAPLLRIVAEKQSLTPAP"
BASE COUNT          618 a          886 c          977 g          573 t
ORIGIN      
        1 tctgcccgat atcgtggcgc gccgtctgct aaagccggtg tctgaggaag ctgcggcgga
       61 acttcgggcc gcgttcagtc agatcgtgcg catgcgcgat gaaacgctcc agacgcttct
      121 gaccacagat gcggcgcgcg ggatgtttga aaaggtctat ccgtttagtc cggcgctgct
      181 ccagacgctg gttgcggtgt catcggcgct tcagcggacc cgcacggcga ttcggctgct
      241 gcttcagctt ttggtggcgc gacgaaatga cttggcactg ggtgaagtcg ttccggtcgg
      301 tgacctgtgg gatgtggtgg cagacggaga cgagccgttt atggatgcga tgcggcttag
      361 cttcgagcgc gcacgcaagc tgtacacgca aaagctgcgg ccccttttgg agtcgacgca
      421 cggcgttcgc tgggatgatc tgcgcaacct gccgacagat gatcccaagc ggaccgcctt
      481 tcgagccgag gaccgtttgc taaaaacgct gctgttggcg gcgctggtac ccgaagtcga
      541 atcgctgcgg agcctgacgg caaaccggct ggcggcccta aactacggca gcatccaatc
      601 aacgattccg gggcgcgaag gccggctgat tctggaacgg attcggtcgt ggagcgcgca
      661 ggtgggcgaa atccgcgtga gcgaggatcg tgacccggtc atccagctcc agctctcggg
      721 ggttgacacc gacagcatct tggagcgagc caagattcag gactcgaccg cgcagcggcg
      781 gcagcttatc aagcggcttc tgtttgctcg cctgggcgtc ttggaacagg actcgctgta
      841 catcgaacat gaggtgatct ggaaaggcac ctcgcgtcgg gtcgaggtgg tctatgccaa
      901 cgtccgcgag ctaaccgatg aatcgcttcg ctcacgcggg gatgagtggc gggtgctcat
      961 cgactttccg ttcgacgaag aaaaccatgg accagccgac gatcgcgcca aggtgcagtc
     1021 gtttctggag cgcggggaaa gtgcgcatac gtttgtctgg ctgccacggt ttttcaaccg
     1081 ccagaccaag cagaacctgg gaacgctggt cattttggat tacctgctga ccggagagcg
     1141 ctttgtcgat cacgcctcgc acctatccgc ggtcgaccga gcagcggcgc gggcgatctt
     1201 ggaaagccag aaaagccagc tagaaggaac catcggacag gcgctgctag aagcctacgg
     1261 aatcgcgggc aacaccgacc gcccaaactt cgtggaaagt tcgcactcga tcgaccagac
     1321 ctttgtgtcg ctggaaccgg cgctttccct gacaccacca gtggcagccg atctaagggc
     1381 tgcgctggaa aaaatcatcg gccagcttct gtcgtggcag ttccctgcgc accccgactt
     1441 cgaggaagag atcaagtctt cgcagatcaa aaaggtctgc gccgaagtcc tgcgggccac
     1501 ccaagtccgc gagggccgca tcattgtgcc caaagagctg agaccgcttt tgcggcagat
     1561 cgcaaaccca atcaagctgg gcgaaatgac cgatggggat gcgttcctgc tcggtcaaca
     1621 ctggcgacag cattttgaca ggctgtacca ccaagaagaa acgatcaaaa aggggacggt
     1681 ttcaggcagg cgcccactga ccgtgcgcat gctgcgcagc tacatcgact tgccgcgcaa
     1741 gatgggcctt ttgcccgagc tgcaaaacct gatcatcgtc gtctttgcgg cccagaccaa
     1801 ccgcgctttt tttcagcatg gcggacccgt cgaaggcaag ctcggcgagc tgcatgacga
     1861 actagagctg cgcgaacaag atctgccgga tgaagccgat tgggtgcagg cccggcatcg
     1921 tgccgaatct gtgtttggac tgaccggcgt gtcggagctg cgcaacgccg tcaacgtggc
     1981 agagctggcg cagaagctgc gcgacaaagt gcaggccgcg cgggaagatg ccaacctgct
     2041 tttgccgcgg cttgccgagt attaccaggc gctgggaatt gtcgaaccgg ctgcctgccg
     2101 actgcaaacc gcccgcacgg cgctgacgct tttggaatcc atgaccagcg gcgatgccaa
     2161 ggatgtgatc cgggcgcttg gcaaagcttc gcttttgcca acgccgcaag ccatcggtgc
     2221 gagtgtctcg caagccaaag tggtcggtgc agccctgggc gagtcgcgcc tgcaagtgat
     2281 ctgcggaacg ctgcaccttt ccgattcgcg acgcgaggcg gcttgcgcga tgctggggac
     2341 gctcaaagac gcccttgtgc gcgatgagct ggcgctgccg cttgcccctg tgctttcggc
     2401 aagtgagcgc acagcggtgt cgctgcttgc cccgaaagcc accgaaagac cgtctctctc
     2461 gcctgccaaa gcagccgccc agggttcacg gtccaacgtg gtggccgaag gcagtcgcag
     2521 tggtctttct ggggaagcca tcggggcggt gctacaagaa atcgcaaagg ccagccaggg
     2581 caaaggccgc aggttgtctg tctcctggtc tgtggaagag gacaaggaat gagcagcggc
     2641 acgcttagcg agtccctgct tcgcgccaag gtcgaagagg tccgccgcaa ggtgcctcag
     2701 gccatggcca ttggcatcaa ggtggatgca ccgtggcgag gctgcgagca gctaacgctc
     2761 ggcgatgcag cgatgcgggt ggtttactgt ccatcggtcc tggccttccg agaacagctc
     2821 gcacaggcgg aaacggaaaa gcttgtcctt ttgaccaacc aaaaggaaca ggaactcggc
     2881 agcgatgtgc tgtatcggtt ggcactttcg cggctgcatg aagtccgacc ctggcaggtg
     2941 attttggaac gcttccaggc acggcgctgg gattcgcgac tcaccaaaga gccgtggatg
     3001 gaagcgcccc ttttgcgaat tgtggcggaa aagcagtcgc taacgcctgc tccg
//
LOCUS       DAORGN010000332         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_20048_length_3037_cov_5.153253, whole genome shotgun sequence.
ACCESSION   DAORGN010000332 DAORGN010000000
VERSION     DAORGN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20048_length_3037_cov_5.153253"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PKL17_21220"
     CDS             complement(<1..1511)
                     /locus_tag="PKL17_21220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47318.1"
                     /translation="MSHAETGQGAGASYRGEFPVAFGRYLLLKRLSRGGMGEIFLAKV
                     GEIQGFEKFVIIKKILPQLAENDDFITRFIDEAQVAIKLNHVNIAQVFEVGRVDGEYF
                     LAIEFIDGRDLRRILRRCRETGTRLPVDLCLFLARDLAAGLAYAHRRQVAKGQALALV
                     HCDISPPNVMVSYEGEVKIIDFGIARSALRTADSNPNIGFGKFGYMAPEQLIRGGVID
                     RRTDIYAAGVVLYELLTGERLYQFPEGTDYRQMARTVCQGNYLQPSARDARLDASMDA
                     ILAKALAVRQEDRYQMAEEFRDALQHKLSQLNPTISADRLAKVMDELFATERQTETDE
                     LAQMRAVDSSAFKDEMVGAAGHTVTFARALEEGQLDNGKTAVTPQVEEEAKTSVVPQA
                     GREGGLAKADMSPISSASLTPSYTVTTSPHRKRYALPLLLGLFACVAVMGGGFLLWPK
                     KTGQKQTSNGPNTKHNVTVEALPEPALPPPDLAMPPPPPPVPQEAPKPPVHKP"
     gene            complement(1568..2368)
                     /gene="bamD"
                     /locus_tag="PKL17_21225"
     CDS             complement(1568..2368)
                     /gene="bamD"
                     /locus_tag="PKL17_21225"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNK47319.1"
                     /translation="MRKLVVLLSLGLTACVTTDEGRKMQDQILELQQRSLKNELGGVE
                     LRNEMQKQADDMRRLLDEAKRLTVSLADASQKAERVQMDIMQVQGKFEDIQKQLEQLQ
                     RTFVDYRSQSDNKLGQLSSTLAKSPPVPESPDALYNEAHQHYTAGKYPEARYMLDAFV
                     ARYPTDARAARAQSMIGDSHYAEGKYRYAIYAYTKILDNFPKSDEVEVAIFKNGQSFL
                     QLKYCKEAKAYFDQFLARYPKSRFRSEVQERIKEIAQKSKDKDACTTK"
     gene            complement(2365..>3037)
                     /locus_tag="PKL17_21230"
     CDS             complement(2365..>3037)
                     /locus_tag="PKL17_21230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47320.1"
                     /translation="DCPGHAEGKEWCVNGKCQACRPTGNDCGPGKACNNGRCESVPGW
                     CGKHSDCPSGLCEQNRCVACKNDSGCPAGGRCNAGKCEQDTRKPCKTSDECAETEDCV
                     AGRCTPAVGRRGGEACQPQTIYFGSDQYELSPANTPVVDSDADCIKKVGKPVSIIGHT
                     DPRGTIEYNLSLSEKRAQAVKRRLGALGIPENNMSTVPRGELDASGSEESGWEKDRRV
                     EVNFR"
BASE COUNT          516 a          926 c          894 g          701 t
ORIGIN      
        1 atcggcttgt gcacgggcgg cttcggcgct tcctgcggaa caggtggcgg cggcggcggc
       61 atcgccaagt cagggggcgg caacgccggc tcgggcagcg cttcgaccgt cacattgtgt
      121 ttggtgtttg gcccgttgga ggtttgcttt tgcccggtct tctttggcca caaaaggaat
      181 ccgccaccca tcactgcaac acacgcaaac agacccagca gaagcggcag cgcgtagcgc
      241 ttgcggtgcg gcgaagtcgt cacggtatag ctgggcgtca ggctggcaga acttatgggg
      301 gacatatccg ctttggccag tccgccttcg cgtcctgcct gcggcaccac cgaagtcttc
      361 gcttcctctt cgacctgcgg tgtcaccgcg gtcttgccat tgtcgagctg gccttcttcc
      421 agcgcgcgcg caaacgtcac ggtgtgtcca gcggcgccga ccatttcgtc tttgaatgcc
      481 gaggaatcga ccgctcgcat ctgcgccagc tcgtcggttt ccgtctgacg ctcggtcgca
      541 aacagctcgt ccatcacttt cgccagccgg tccgccgaaa tcgtcggatt cagctgcgac
      601 agcttgtgct ggagcgcgtc acgaaactct tccgccatct ggtaacggtc ttcctgccgc
      661 accgccagcg ctttggccaa aatggcgtcc atcgacgcat ccagccgggc atcgcgcgcc
      721 gacggttgca gatagtttcc ctgacagacg gtgcgtgcca tctgccggta gtccgttcct
      781 tcgggaaact gatacagccg ctctccggtc agcagctcgt acagcaccac gcccgcggcg
      841 taaatgtcag tccgcctatc gatgacaccg ccgcgaatga gctgttcggg cgccatgtat
      901 ccgaacttgc caaagccgat gttgggattg gaatccgcgg tgcgcagcgc cgaccgagcg
      961 atgccaaagt cgatgatttt gacctcgcct tcataagaca ccatgacgtt gggcggcgag
     1021 atgtcacagt gcaccagcgc gagcgcctgt cctttggcca cttgtcgacg gtgcgcatag
     1081 gccagaccgg cggccaaatc gcgggccaaa aacagacaca gatcgaccgg aagtcgggtt
     1141 ccggtttcac ggcagcgacg cagaatccgg cgcagatcgc gaccatcaat aaactcgatc
     1201 gctaggaagt attccccgtc gacgcgaccg acctcgaaca cctgcgcaat gttgacgtgg
     1261 tttagtttga tggcgacctg cgcttcatca ataaagcgcg tgatgaagtc atcgttttcg
     1321 gcaagctgcg gcaggatttt tttgatgatg acgaactttt cgaagccctg aatttccccg
     1381 actttggcca ggaaaatttc gcccatgcct ccgcgtgaaa gtcgcttgag cagcagataa
     1441 cggccaaatg cgacgggaaa ctcgcctcga taggaggcgc ctgcgccctg acctgtttct
     1501 gcgtgcgaca tcggcggcat cttgtttgcg gcttctcttt ctgcggtgcg aaggcgattt
     1561 cgtcagctta cttcgtggtg caggcgtcct tgtctttgga cttctgcgcg atttctttta
     1621 tccgctcttg gacttcgctg cggaagcggc ttttggggta gcgggccaag aactgatcaa
     1681 agtaagcctt cgcttccttg cagtatttga gctgaaggaa ggactggccg tttttgaaaa
     1741 tcgcgacctc gacctcgtcg ctcttgggga aattgtcgag gatcttggtg taggcgtaga
     1801 tcgcgtagcg atatttgcct tcggcgtagt gggaatcgcc gatcatcgac tgtgcccgcg
     1861 ccgcccgtgc gtccgtcgga tagcgcgcca cgaatgcatc aagcatatag cgcgcctcgg
     1921 gatattttcc ggcggtgtaa tgttggtgcg cttcgttata aagcgcgtcg gggctttccg
     1981 gaaccggcgg actcttcgcc agcgtgctgc tgagctgtcc cagcttgttg tccgactggc
     2041 tgcgatagtc gacgaaggtg cgctgaagct gctcaagctg cttctgaatg tcctcaaact
     2101 tgccttgcac ctgcataata tccatctgca cgcgctcggc tttttgtgac gcatcggcca
     2161 agctcaccgt cagtcgcttg gcttcgtcca gcagtcgacg catgtcgtcg gcctgcttct
     2221 gcatttcgtt gcgtagctca accccaccca gctcattttt gagcgagcgc tgctgcagct
     2281 cgaggatctg atcctgcatc ttgcgacctt catcggtcgt gacgcaggcc gtgagtccca
     2341 gcgacagcag caccacaagc tttctcatcg gaaatttacc tccacgcggc ggtctttttc
     2401 ccaacccgac tcttcgctgc ccgaagcatc aagctcgcct cgtggcaccg tgctcatgtt
     2461 gttttccgga atgcccagcg ccccgagccg acgcttcacc gcctgggctc gtttttccga
     2521 caggctcagg ttgtattcga tggtgccgcg cggatcggtg tggccgatga tgctgaccgg
     2581 cttgccgact tttttgatgc agtcggcgtc cgagtcgacc accggagtgt tggcgggcga
     2641 tagttcgtac tggtcgctgc cgaaatagat cgtctgcggc tgacacgctt cgccaccgcg
     2701 gcgaccaacg gcgggcgtac agcgaccggc gacgcagtct tcggtttcgg cacactcatc
     2761 gctggttttg catggcttgc gggtgtcttg ttcgcacttg ccggcgttgc agcgtccgcc
     2821 tgctgggcag ccactgtcgt ttttgcaggc gacgcagcgg ttttgctcgc agaggcccga
     2881 tggacagtcg ctgtgcttgc cgcaccagcc gggcaccgat tcgcagcgac cgttgttgca
     2941 ggctttgccg gggccgcagt catttcccgt gggtcgacag gcctggcact tgccgtttac
     3001 acaccactct ttgccttcag cgtggccggg gcagtcg
//
LOCUS       DAORGN010000333         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_20406_length_3007_cov_6.021003, whole genome shotgun sequence.
ACCESSION   DAORGN010000333 DAORGN010000000
VERSION     DAORGN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20406_length_3007_cov_6.021003"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKL17_21235"
     CDS             complement(<1..744)
                     /locus_tag="PKL17_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNK47321.1"
                     /translation="MPSSFSSAIDPNRIVCLRQGSPRTSGRYVLYFCQIARRAEQNPA
                     LDYAIAWANHLRLPVVVYEGLRPDYPHASARHHRFILDGVRDFALRLATRGIAHVFYL
                     PKRGERFAMLAALADQAALVVTDDHPTFVVPRHNRKLAQTLDCPLYAIDGCDVVPLRT
                     FSRAFPTAAALRPHLHRLWPKLLAETLDEPQPIHDSLSLFLPALPGLVRGVDVSDVPA
                     DLDRQIVETGVDASVTAVAFVRGGRTAGLL"
     gene            complement(818..1630)
                     /locus_tag="PKL17_21240"
     CDS             complement(818..1630)
                     /locus_tag="PKL17_21240"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNK47322.1"
                     /translation="MKRVGKMWLLGGVSLLGLVMPWQVRANDSSAVETVAVVDRARAA
                     ERGGPRSGGEVRVADGNASKGADTASEKAADKAADKGPEKSADKPENYIDKMFDGGPG
                     NDRLTKMHAASADLLNVLRSDGHYAQFVQLAESSGFDKTLHGRGPFTVFVPTDEAFAK
                     LPAEMRDRLKQDPTRHRTLLAYHVIRGLVSKDVLNHLRNVKTMSGSVVNVDYTSGIRI
                     NRAHIVSGDHFGNNGMFHAIDEMLIMPERPAQPVKPVAGPRKKPLRKPAPTS"
     gene            complement(1647..2039)
                     /locus_tag="PKL17_21245"
     CDS             complement(1647..2039)
                     /locus_tag="PKL17_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNK47323.1"
                     /translation="MKTHTVLLVDDDIHNQKIFETVLRHSGFQVRQAGNGEDALREAR
                     THRPDLILMDLSIPVIDGWECTRQLKADESTRNIIILALTAHAMHGDEERALAAGCDG
                     YLSKPISPRKLVEEVKSRLQVTRGALPS"
     gene            complement(2060..>3007)
                     /locus_tag="PKL17_21250"
     CDS             complement(2060..>3007)
                     /locus_tag="PKL17_21250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNK47324.1"
                     /translation="HELVLNNEALAAQRDQMQQQRDQLVQQRNQLAEQRDQLEQQSYQ
                     LRIQRNQLREVNRLKSEFLANVSHELRTPLNAIMGYTELIRDGVYGLITGEAHDATDG
                     VLASSANLLRLINQILDLSRIEAGKMDVHAESVELSALVAAVIKEAEAMGRDRPYRVQ
                     LSCPRDLRVQTDPAKLQQILTNLVANAIKFTSQGSVNVVVGADDAEAEYVTVSVIDTG
                     IGIRPEHLEIIFEEFRQVDGSSTRRFGGTGLGLAIARRLAEMLGCVLEVKSTFGEGST
                     FSLRMPRQLGGGRRLSRRSVDMDSNPSIDAPQRAQGVRE"
BASE COUNT          585 a          957 c          871 g          594 t
ORIGIN      
        1 caaaagccct gccgtccgtc cgccacgcac gaaagccacc gcagtcaccg acgcatccac
       61 gccggtttcg acgatctggc gatccaggtc ggcgggcaca tccgatacgt caacaccgcg
      121 cacaagcccc ggcagcgctg gcaaaaaaag tgacaacgag tcgtggatcg gctgcggttc
      181 gtcgagcgtt tccgcaagca gcttgggcca cagccgatgc aggtgcggac gcagcgctgc
      241 cgccgtcgga aacgctcgcg aaaatgtccg cagcggcacc acatcacaac catcaatcgc
      301 gtacagcggg cagtcgagtg tctgcgccaa tttgcggttg tgccgcggca ccacaaacgt
      361 cggatggtcg tcggtgacca ccagcgccgc ctgatccgcc agcgccgcca gcatcgcaaa
      421 ccgctcaccg cgctttggca gatagaaaac gtgggcaatc cctcgcgtcg caagtcgcag
      481 cgcaaaatcg cgtactccgt cgaggataaa gcggtgatgg cgcgcactcg catgcggata
      541 gtcggggcgc agtccttcat acacgacgac gggcagccgc aggtgattgg cccaagcaat
      601 cgcgtagtcg agcgccggat tctgctcggc gcgtcgcgca atctgacaaa aatagagaac
      661 gtagcgtccc gacgttcgag gcgacccctg ccgtagacaa acaattcgat tgggatcgat
      721 ggcagacgaa aacgaggaag gcatatcgtc taggatgccg cctgggacgc cgccaaagcg
      781 ccaagcgctg cgggctgtcc gacgatagca aacgcagcta tgaagtcggt gccggcttgc
      841 gcagcggttt tttgcggggt ccagcgactg gcttgactgg ctgcgccggt cgctctggca
      901 tgatcagcat ctcgtcgatg gcgtggaaca tgccgttgtt gccaaagtgg tcgccagaca
      961 cgatgtgcgc gcggtttatg cgaatgccgc tcgtgtaatc gacgttgacc accgagccgc
     1021 tcatcgtctt gacgttgcgc aggtgattga gcacatcttt cgagacgagc ccgcggatca
     1081 catggtaggc gagcagcgtg cgatgacggg tcgggtcttg cttcaaacgg tcccgcatct
     1141 cagccggaag tttggcaaac gcttcgtccg tcggcacaaa caccgtaaac ggcccgcgtc
     1201 cgtgcagcgt tttgtcgaag cccgatgact cagcaagctg gacaaactgt gcataatgac
     1261 cgtcgctgcg cagcacattc aaaagatcag ccgatgctgc gtgcatcttg gtcagtcgat
     1321 catttcccgg tccgccgtcg aacatcttgt cgatgtagtt ttccggtttg tcggctgact
     1381 tttctggccc cttgtccgcc gctttgtccg ccgctttttc gctggctgta tcggcgccct
     1441 tgcttgcatt tccgtcagcg acccggactt cgccaccgct tctcggtccg ccgcgctcag
     1501 ccgctcgagc ccgatcgacg acggccaccg tctcgactgc cgacgaatca ttggccctga
     1561 cttgccacgg catgacaagc ccaagcagcg acacgccgcc gagcagccac atcttcccaa
     1621 cgcgcttcat ctacttcccc cagagcttat gaaggcaacg cacctcgcgt cacttgcaga
     1681 cgcgatttta cttcttcgac cagtttgcgc ggcgaaattg gcttcgacaa atagccgtca
     1741 cagcctgccg ctagcgcgcg ctcctcgtcg ccatgcatcg catgcgcggt aagcgccaaa
     1801 atgatgatgt tccgcgtact ctcgtcggct tttagctgcc gcgtacactc ccagccgtcg
     1861 atgacaggaa tcgacaagtc catcaggatc aggtcgggcc gatgcgtgcg cgcttcccgc
     1921 agcgcatctt ctccgttgcc ggcctggcgc acttgaaagc ccgaatgacg caacaccgtc
     1981 tcgaaaatct tctggttgtg aatgtcatca tcgaccagaa ggacggtatg agttttcatc
     2041 gcggctgaca tcgtggctct tactctctca cgccttgggc gcgctgtgga gcgtctatgc
     2101 tgggattgct atccatatcg acggagcgtc gcgacagcct tcgaccccca ccaagctgac
     2161 gcggcatgcg cagcgagaag gtcgacccct ccccgaacgt acttttgact tcaagcacac
     2221 agccgagcat ttcggccagg cgacgcgcaa tcgccagtcc gagcccggtg ccgccaaatc
     2281 gtcgcgtcga gctgccgtcg acttggcgga attcctcgaa aatgatctcg aggtgttcgg
     2341 gacggatgcc gatgccggtg tcgatgaccg ataccgtcac atattccgcc tccgcgtcgt
     2401 cggcaccgac caccacgttg accgaaccct gcgacgtgaa cttgatcgca ttggccacca
     2461 agttggtcag gatctgctga agcttggccg gatcggtctg cacccgcaga tcccgcggac
     2521 acgaaagctg gacacgatag gggcgatcgc gccccatcgc ttcggcttct ttgatgaccg
     2581 ctgcaaccag tgccgacagc tccaccgatt cggcgtggac atccatcttg ccggcttcga
     2641 tccgcgacag gtcgaggatt tggttgataa gccgaagcag gttggccgaa gacgccagca
     2701 caccgtcggt ggcatcatgc gcttcacctg taattaggcc atagacgccg tcgcgaatca
     2761 gctcggtgta gcccatgatg gcgttgagcg gcgtgcgcag ctcgtgggaa acgttggcca
     2821 gaaactcgga cttgagccgg tttacttcgc gaagctggtt gcgctgaatc cgcagctgat
     2881 acgactgctg ctccagctga tcgcgctgct cggcaagctg gttgcgctgc tggacaagct
     2941 gatcgcgctg ctgctgcatc tggtcgcgct gcgccgccag cgcttcgttg ttgagcacca
     3001 gctcgtg
//
LOCUS       DAORGN010000334         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_20794_length_2975_cov_5.632192, whole genome shotgun sequence.
ACCESSION   DAORGN010000334 DAORGN010000000
VERSION     DAORGN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20794_length_2975_cov_5.632192"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKL17_21255"
     CDS             complement(<1..299)
                     /locus_tag="PKL17_21255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNK47325.1"
                     /translation="MALRKFLVDAQAANQRLDRFVATHAGVSRGAAMRLIADGLVQVD
                     GRVGKKGVSLLAGQTVALPEDAQDDVSTPPVAEPDLPLVVLYEDADVVVVAKPQG"
     gene            complement(315..1175)
                     /locus_tag="PKL17_21260"
     CDS             complement(315..1175)
                     /locus_tag="PKL17_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47326.1"
                     /translation="MRRLLAYLVLSLSLGIALPSFAQSEPTTTAPAATGAPVQNNAPM
                     FAAPVAVPSQVTPHALALRARWVTVPGWSLNAFLDAHTQLNDGWSVGLEYLYRRSGFD
                     VVLSVDYSWLEAASGNFLGRGNAPATDTHFTRFDKLSSLSFDASLIGHWNLTPWLEFR
                     LGAGLGIGYVFGNIYQITNNSGCTADNAGDSTKCFPKNVGAIETVDSGTIDRLNSVAC
                     PGDDSSLDTPNKPCTRSTQSYPFNPRIVPVLNTMFGFRFKLQDHVYWHLDAGWRLVGF
                     YLGGGPEFRF"
     gene            1349..2938
                     /locus_tag="PKL17_21265"
     CDS             1349..2938
                     /locus_tag="PKL17_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNK47327.1"
                     /translation="MQALHVAPQLPLCEADGVSVRYDQKLANERVSLTVQPGEVVALL
                     GENGAGKSTLLHALYGLWPICDGVIRYRGQAVVPSPERAIAAGLGLIHQHFLLVPTLT
                     VAENVVLGCEPRRFGLLVDEKKAIEDVAALGRAHGLAVEPRRLVSELSVGEAQRVEIL
                     KALYRGARLLLLDEPTAVLTPAEATALLSTLRGLMDKPQDRDRALLIVTHKLDEVLQV
                     ADRAVVMRGGRVVEQFVRSEFSASGIARAMVGRQVSAVVRSPRQADSADGVSSANALE
                     VDHLVVERDGSEWVRGVSLSLKPGRILGIAGVAGNGQSELVSAIAGLLPIKSGRVRLC
                     GTDVTDGDVKLRSQLGLALVPEDRHRHGLVLDMTLGENLILGHDADFARGPGKLLLDG
                     KKIRTVAEQVLSDADVRPAAQGALARSLSGGNQQKLVMSRALTMGGLPKVLIAAQPTR
                     GVDIGAIESLHRTLLRSRDAGCAVLLLSAELSELQALADHILVMYRGQIVADLDNDPA
                     HPVRREQLGELLTGAFVQTQN"
BASE COUNT          538 a          838 c         1006 g          593 t
ORIGIN      
        1 tggccttgcg gtttggccac caccaccacg tcggcgtctt catacaaaac cacaagcggc
       61 agatccggtt cggccactgg cggcgtcgat acatcatcct gtgcgtcttc gggcagcgcc
      121 accgtctgac cggccagcag cgacacgccc tttttgccga cccggccatc gacttgcacc
      181 agaccgtcgg cgatgagcct cattgccgcg ccgcgactga cccccgcatg ggttgcgacg
      241 aaccgatcca gtcgctgatt tgcagcttgt gcatctacca aaaacttccg caaggccacg
      301 gcgtgctccc tcggttagaa acggaactca gggccaccgc ccaggtaaaa tccgacgagc
      361 cgccagcccg catcgaggtg ccaatacaca tggtcctgga gcttgaagcg gaagccgaac
      421 atcgtgttta gcaccggcac aatccgggga ttgaacggat acgactgcgt cgatcgcgtg
      481 cacggcttgt tcggcgtatc cagcgacgaa tcatcaccgg ggcaagccac cgagttcaga
      541 cgatcgatgg tgcccgaatc gacggtttca attgccccga cgtttttcgg aaagcacttg
      601 gtcgaatcgc cggcattgtc ggcggtgcag cccgagttgt tggtgatctg atagatgttg
      661 ccaaagacgt aaccgatgcc gagaccggca ccgagtcgga actcgagcca gggcgtcagg
      721 ttccagtggc cgatgagcga cgcatcgaac gacagcgacg acagtttgtc aaagcgggta
      781 aagtgggtgt ccgtcgcggg ggcgttgcct cggcccagga agttacccga cgctgcttcg
      841 agccacgagt aatcgaccga caacacgaca tcgaagccgg atcgcctgta gaggtattca
      901 agcccgacgg accagccgtc attgagctgg gtgtgggcat cgagaaacgc attgagcgac
      961 cagcccggca ccgtcaccca gcgcgcccgc agtgccaggg cgtgtggtgt cacctgggac
     1021 ggaacggcga ctggggctgc aaacatcggt gcattgtttt ggaccggcgc gcccgtcgcg
     1081 gctggtgccg ttgtggttgg ctcactctga gcaaagctgg gcagcgcaat cccgagggac
     1141 agcgaaagga caaggtaggc aagcaagcgt cgcatcgagg ttcctcccga aactggttgc
     1201 gtcttggcgg ggtgaatggt cgcgaggcta gcactttttc cgctgtcgcc cactgcgaag
     1261 tttgtccccg gtgggcaagt cggtgcaggc gcacaggaat cgacggacag cgcttgcagt
     1321 ggcggcgatt tgttgctctc ttttgcgcat gcaagctctc catgtggcgc cgcagctgcc
     1381 tttgtgcgaa gcggacggtg tatcggtgcg ctacgatcaa aaactcgcca atgagcgggt
     1441 ttctctgacg gtgcagccag gggaagtggt ggcgctgctc ggggaaaatg gagcgggcaa
     1501 gtcgacgctt ttgcacgcgc tgtatggact ttggccgatc tgcgacgggg tgattcgcta
     1561 tcgcggtcaa gcggtggtgc cgtcgccaga gcgggcgatc gcggcgggac ttggactgat
     1621 tcatcagcat tttttgctgg tcccgacgct gaccgtggcg gaaaacgtgg tgctgggctg
     1681 tgaaccccgg cgatttggtc tgctcgtcga cgaaaagaaa gcgatcgaag atgtggcggc
     1741 gctgggtcgc gcgcacggtc tggctgtcga accgaggcgg ctggtttcgg aactttccgt
     1801 cggggaagcc cagcgggtcg agatcctaaa agccctgtat cggggcgcgc ggcttttgct
     1861 tttggatgaa ccgacggcgg tgctgacccc ggcggaagcg accgcgcttt tgtcgacgct
     1921 gcgtggtctt atggacaagc cgcaagaccg ggatcgcgcg ctgttgatag tcacgcacaa
     1981 gctcgatgaa gtgctgcaag tggcggatcg ggcggtggtg atgcgcggtg gtcgcgtcgt
     2041 cgaacagttt gtgcgcagcg agttttcggc gtcgggcatt gcacgggcga tggtcggtcg
     2101 acaggtttct gccgtcgttc gttcgccgcg ccaggccgac agcgccgatg gtgtgtcatc
     2161 ggcaaatgcg ctcgaagtcg accatttggt cgtcgagcgc gatggcagtg aatgggtgcg
     2221 tggcgtctct ttgtcgctca aaccgggtcg cattttgggc atcgctggcg tcgctggaaa
     2281 tggtcagagc gagctggttt cggcgatcgc gggtcttttg ccgataaagt ccggtcgtgt
     2341 gcggctgtgt ggcaccgatg tgaccgacgg ggatgtgaag ctgcgttccc aacttgggct
     2401 ggcactggtg cccgaagacc gccatcgcca tggcttggtg ctcgacatga cgctgggtga
     2461 aaacctgatc ttgggtcatg acgccgactt tgcgcgtggt ccgggcaagc tgcttctcga
     2521 cgggaaaaaa atccgcacgg tggcagagca agtgctttcc gatgccgatg tgcggcctgc
     2581 ggcgcaaggt gcgctggcgc gatcgctgtc gggtggcaat cagcaaaaac tggtgatgtc
     2641 gcgggcgctg acgatgggcg ggctgcccaa agtgctgatt gcagcccagc caacacgagg
     2701 ggtcgacatc ggcgccatcg agtcgctgca ccggacgctg ctgcgttcgc gtgacgctgg
     2761 ctgcgcggtg ctgcttttgt ccgccgagct gtcggagctg caagcgctgg cggaccacat
     2821 tttggtcatg tatcgcggcc agatcgtcgc tgatctcgac aatgatccgg cacatccggt
     2881 tcggcgcgaa cagcttgggg agcttttgac cggcgccttt gtccaaacgc agaactagcg
     2941 cggcagggcg attgcttcgt cgctgaagtg gcggg
//
LOCUS       DAORGN010000335         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_21479_length_2919_cov_5.267458, whole genome shotgun sequence.
ACCESSION   DAORGN010000335 DAORGN010000000
VERSION     DAORGN010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21479_length_2919_cov_5.267458"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKL17_21270"
     CDS             <1..242
                     /locus_tag="PKL17_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47328.1"
                     /translation="CGDCKTDADCPAMNPACDPTKKLCSPCRVDSATLPHLGCANPNP
                     ACKTNTNPTLNMCVECTQSSHCMGMKTCNAMNNCV"
     gene            complement(268..2067)
                     /locus_tag="PKL17_21275"
     CDS             complement(268..2067)
                     /locus_tag="PKL17_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47329.1"
                     /translation="MKRLRELLRLVLLSKWLPVFVFGLALVLCLPALSAGLMLDDIPM
                     QMMFQPDFPHQPGGPRGDWDLFRFLGPDRSYLRYQMELGLMPWWTAPTIRIAFFRPLS
                     SLHMAIDFRNWADKPWLLHLESLLLYAGLALATCRLYRQLFGVTIAAGLAAVMFAIDD
                     AHSLPVVWLSNRNALWATCLGVLSLSSFVAMRRGTFSAGKWLSPILFALALLSGEAAL
                     GAAAYLFAYVVWVEEGSLWARLSLLVPYVAIGLVWAGIYGALGYGTVDSGMYIDPLRS
                     PVQYLIAVGSRLPLLLLAQLAVPPADLWMQLSPEVQVQAGLAAWLLLALAGLFGVRLL
                     RGDRLSGFFATGMILSLLPVCSAWPMDRLLLFSGLGAFGLLAQLLLRLRNGLTEPALS
                     RPGLRKLAIGVLVLVHIVLAGLLLPARIAFINKTFGGLDERAAMTLPSHEKLHGKTLV
                     FVNSPDPLVASYSVVYRYLHGRTLIHDLRLLSSVLRGSLTITRTDERTLVVWPSAGFF
                     QEPLSRVFRSATLPFAAGDKVTLSNLTAEVLEASSEGTPQKVAYHFDRPLEDAQLTFV
                     SWVDRGFVPFVPPAVGQTVTLPAIDYAKALGSK"
     gene            2215..>2919
                     /locus_tag="PKL17_21280"
     CDS             2215..>2919
                     /locus_tag="PKL17_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47330.1"
                     /translation="MLLGRRSPVVFGVCSILLSIQPACQLRSASQSDESALPRDSRDP
                     PPASLQTFLKDKETRATPGLFVTYAYRQKTYLLVQKKQLGKPFLLTGLVSQGAGMSAG
                     TPLGQPTGTGLLEEVVTFVRQGNRLFLIQKPHRHLTSETMKQAVEQTFSPFVMASLPI
                     VAEDKPSGRSSGKASGDLLLAVDDLLLEDLAAAGMAGRDAIKPSRRLSYVEASSGTAV
                     STSLRVPLSSPALRVSM"
BASE COUNT          689 a          969 c          832 g          429 t
ORIGIN      
        1 tctgcggtga ctgcaagacc gacgcagact gcccggcgat gaatccggcc tgcgacccga
       61 ccaagaagct gtgctcgcct tgccgcgtcg acagtgccac gctgccccac ctgggctgtg
      121 ccaatccaaa cccagcgtgc aagaccaaca ccaacccgac gctcaacatg tgcgtcgagt
      181 gcacgcagtc gtcgcactgc atgggcatga aaacctgcaa cgcaatgaac aactgcgtct
      241 agcccacggc agcccgcgcg atccctccta cttgcttccc agtgccttgg cgtaatcaat
      301 cgccggcagc gtcaccgtct gacccactgc cggcggcaca aacggcacaa agccccgatc
      361 aacccacgaa acaaacgtga gctgcgcgtc ttccagtggc ctgtcaaagt ggtacgcaac
      421 cttctgcggc gttccttcgc tgctggcctc cagcacttcg gcggtgaggt tcgacagcgt
      481 gactttgtcg ccagcggcaa acggcagcgt cgcgctgcgg aacacccggc tgagcggttc
      541 ctgaaaaaag ccagcgctcg gccacaccac aagcgtccgt tcatcggtcc gcgtgatggt
      601 gagagatcca cgcaacaccg atgacaagag ccgcaagtca tgaatcagcg tgcggccatg
      661 cagatagcgg tacaccacgg aatagctggc gaccagcgga tcgggcgaat tgacgaacac
      721 cagcgttttt ccgtggagtt tttcatgcga cggcagcgtc atcgcggccc gctcatcaag
      781 cccgccaaag gtcttgttga taaacgcgat gcgggcaggc aggagcagcc cagccaagac
      841 gatgtgaaca agcaccaaaa ccccgatggc cagcttgcgc agtcccggtc gtgacaaggc
      901 cggctcggtc agcccgtttc gcagccgcaa aagaagctgc gccaacagcc caaacgcacc
      961 cagccccgaa aacaaaagca gccgatccat cggccacgcc gagcagaccg gcaaaagcga
     1021 caggatcatg ccggtggcaa aaaaaccgct cagccggtcg ccgcgcaaaa gccgaacgcc
     1081 gaaaagcccc gccagcgcga gcagaagcca agccgcaaga cccgcctgca cctggacttc
     1141 cggcgacagc tgcatccaca aatccgccgg cggcaccgca agctgggcca agagcaaaag
     1201 cggcaaccgc gaccccaccg cgatcagata ctgcacaggc gagcgcagcg gatcgatgta
     1261 catgccgctg tcaaccgtcc cgtagccgag cgcgccatag atccccgccc agacaagccc
     1321 gatcgccaca tacggcacaa gcagcgaaag ccgagcccac aaggaacctt cttcgaccca
     1381 caccacgtag gcgaacaggt acgcagccgc cccgagcgcc gcttccccag acaagagcgc
     1441 cagcgcaaac agaatcggag acagccactt tccggctgaa aacgtcccgc gccgcatcgc
     1501 gacaaagctc gacagcgaaa gcacgcccaa acaggtggcc cacagcgcgt tgcgattcga
     1561 cagccacacc accggcagcg agtgcgcgtc atctatcgca aacatcaccg ccgcaagacc
     1621 cgccgcgatg gtcacgccga aaagctggcg atacagccga cacgtcgcaa gcgccagccc
     1681 ggcgtacaaa agcagacttt ccaggtgcag aagccacggc ttgtccgccc agttgcgaaa
     1741 atcgatcgcc atgtgcagcg agctcagcgg gcgaaaaaac gcgatccgga tggtcggcgc
     1801 cgtccaccac ggcataagcc ccagctccat ttggtagcgc agatagctgc gatcaggccc
     1861 aaggaagcga aacaagtccc agtcgccgcg cggcccccct ggctgatgcg gaaagtccgg
     1921 ctgaaacatc atctgcatcg ggatgtcgtc gagcatcaga cccgccgaaa gcgccggcag
     1981 acacagcacc agcgcaagac cgaacacaaa gaccggcagc cacttcgaca gcagcacaag
     2041 acgcagaagc tcgcggagac gtttcatcga ctggaaacct cggtgtaaga cccagacagc
     2101 acgcgacagg tggacgccat catatcgatc gagcgtcgcg aagtggggcc ccctgtggtg
     2161 acgaaatttg tgccataatg ggaagctatt ggcaggactt gggagggggt tcctatgctg
     2221 cttggtagac ggagtccggt tgtatttggc gtatgttcga tcctgctttc catccagccc
     2281 gcttgtcagc ttcgcagcgc cagtcagtcc gatgaatccg cgcttccccg cgacagccgc
     2341 gatccgccgc ccgcgtcgct ccagacattt ctaaaagaca aagaaacccg cgcaaccccc
     2401 ggtctgtttg tcacctacgc ctatcggcag aagacgtatc tgctcgtgca aaaaaagcag
     2461 ctgggcaagc cgtttctgct caccggactt gtcagccagg gcgccgggat gagcgcaggc
     2521 acgccgctcg gacagccgac aggcaccgga cttctggaag aagtcgtgac gtttgtgcgg
     2581 cagggcaatc ggctgttttt gattcaaaag ccccatcgcc acctgacatc ggaaacgatg
     2641 aagcaggcgg tcgagcagac gttttccccc tttgtgatgg cgtcgctgcc gatcgttgca
     2701 gaagacaagc cgagcggtcg atcaagcggg aaagccagcg gcgatcttct gctggctgtc
     2761 gatgaccttt tgctcgaaga cttggcggcg gcgggaatgg cgggacggga cgcgatcaaa
     2821 ccgtcgcggc ggctcagtta tgtcgaagca tccagcggta cagcggtcag cacatcgctg
     2881 cgcgtgccct tgagcagccc agcactgcgg gtctcgatg
//
LOCUS       DAORGN010000336         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_21540_length_2914_cov_5.117873, whole genome shotgun sequence.
ACCESSION   DAORGN010000336 DAORGN010000000
VERSION     DAORGN010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21540_length_2914_cov_5.117873"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKL17_21285"
     CDS             <1..213
                     /locus_tag="PKL17_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47331.1"
                     /translation="DQLERKLPLRDLPLSAAEHSLVAGCRFLSAKPLLVLLNLEGKDL
                     DQPIPSDVESYLKSHKLTALPIRGIA"
     gene            361..1812
                     /locus_tag="PKL17_21290"
     CDS             361..1812
                     /locus_tag="PKL17_21290"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNK47332.1"
                     /translation="MALVSMLLVGAVVATTSWVFTRSLRTAGEREVDEELASDVLLVT
                     YAIQRAQIPEETLAELPRPGLSWLVVRDGRLSRARLTSDVQRDRLTVETRCITATATQ
                     DRDLILSGQRYRSVYAAAGSGTSVCLLRSVDAVLSRSKTVQTTLFAVGRGAVLLALLV
                     AGLWGLALMERVRRILAAMQQVASGDFDVEVPEKGHDEIAAIARSLVHMIRKLRGAQA
                     ELQSSAAHEREAALAAKLRGQLLPLQPSVNGFEFAARIIPAPNGGGDFYETYQGESSS
                     FVAVGNISGSGVGPGLSMVIVDSLLLATLRACRDAVPSPTAILQQVGAALFESLRKRM
                     HDDQMVSVLLLRHDGGGRVTYSGSVPLLLLYRDSTRTVEKLEVARFGLAVSKQFDDRL
                     TDRSVQMTAGDLIVLVTDGITEAHDAQGERIALDRLSDAIAKHAQRLTAAELRDHLLD
                     VVTEQVGSQNRDLTVVVLRRNPSTGLSQQGSRP"
     gene            1809..>2914
                     /locus_tag="PKL17_21295"
     CDS             1809..>2914
                     /locus_tag="PKL17_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNK47333.1"
                     /translation="MMSEHRIPIFIAGGGILCLLLQLVLSLADSPARRAQHVAQKVAI
                     ESVYLRSEKRAERLAQRLNQALAEGDQAKLQAELDLASQADPDLVEIGVVDHDLSVVA
                     SSEKGRIGKPLGGHAESLVKKAVAANFARQVESLPQNQRVVLALPVTGQPGSSGLLYL
                     ELTTAEMEDGLGKVVTTVQPQRVPQWLTIGLGILGVGVALGMWLLNDRKRERQSMVVQ
                     EVAEQMAMGHFETRLSPAEVPSFAAIAERLNMMADQAALLQQTQLEGTAHTSRMAQEL
                     EDAQIVQQTLMPDVRRISRGALQLCGVYRSASKLSGDWWHYYPLDEHRTMLVMADVVG
                     HGIGSAVVGAMAYGCAQQLHQEQGPKLRPELLLSR"
BASE COUNT          563 a          850 c          944 g          557 t
ORIGIN      
        1 gaccagcttg agcgcaaact gccgctgcgc gatctgccgc tgagcgccgc cgaacattcg
       61 cttgtggccg gctgccgatt tctcagcgcc aaaccactgc tcgtgctgtt aaacctcgaa
      121 ggcaaggatc tcgaccagcc gatcccgagc gacgtcgaga gctacctaaa aagccacaag
      181 ctcaccgccc tgcccatccg cggcatcgcc taacccgtct gcaagcaatc ggtttgaaac
      241 ggccaccctg acgacgtctt tacgacggag tgggaaaagt ggtttaatga aatctttctc
      301 agactttgcg tttttcgcag gggacccggg ggggtaacag ctttgaagct ggggatcaag
      361 ttggcgctgg tctcgatgct gcttgttggc gctgtggtag cgacgacaag ctgggtgttt
      421 acgcgatcgc tgcgaacggc gggagagcgt gaagtcgacg aagagcttgc gtccgacgtg
      481 ctgcttgtca cttacgcgat ccagcgtgcc cagattcccg aagagacact cgcggagctg
      541 ccacgtccgg gcttgtcctg gctggtggtt cgggacggaa gattgagccg cgcccgcctg
      601 accagcgatg ttcagcgcga ccgattgaca gtggaaacgc gctgcatcac cgcaacagca
      661 acacaagacc gggatctgat cctatcgggg cagcgctatc gcagcgtgta tgcagcggcg
      721 ggctcaggca caagtgtctg tctgttgcgc tctgtcgatg cggtcctttc gcgatccaag
      781 acggtgcaga cgaccctgtt tgcagtcggt cgcggtgcgg ttctgcttgc gctgctcgtc
      841 gctggactgt ggggattggc gctgatggag cgggtgcgac gcattttggc ggcgatgcag
      901 caagtcgcaa gcggggattt cgacgtcgag gttcccgaaa aaggccacga cgaaatcgcc
      961 gcgattgccc gcagtctggt ccacatgatt cgcaagctgc gcggtgccca ggccgagcta
     1021 caaagcagcg cggcacacga acgagaagcc gccctcgcgg caaagctacg tggccaactg
     1081 ctgccccttc agccgtcggt caacggcttt gagtttgcgg cccgcatcat cccggcgcca
     1141 aatggcggcg gcgattttta cgaaacctac cagggtgaat cgtcgagctt cgtcgcggtc
     1201 ggcaacatct cgggcagcgg cgtcgggccg ggtctgtcga tggtgatcgt cgatagcttg
     1261 ctgctggcaa cacttcgcgc gtgccgggac gcggttccgt cgccaacggc cattttgcaa
     1321 caggtcggcg cggcgctgtt cgagagcctg cgcaagcgca tgcatgatga ccagatggtg
     1381 tccgtgctcc tgctgcgtca cgacggcggc gggcgcgtca cctattcggg cagtgtgccg
     1441 cttttgctgc tgtaccgcga cagcacccgc acggtggaaa agctggaggt tgcgcgcttc
     1501 gggctcgcgg tatcaaagca gtttgacgat cggctgaccg accgttcggt gcagatgacg
     1561 gcgggcgact tgatcgttct tgtgaccgat ggaatcaccg aagcacacga cgcacagggc
     1621 gagcgaattg cacttgatcg cctctccgac gccatcgcca aacatgcgca gcgactgaca
     1681 gctgccgagc tgcgggatca cttgcttgat gtcgtgaccg agcaggttgg cagccaaaac
     1741 cgtgacctta ccgtcgtcgt gttgcgccgc aatccatcga cgggactgag ccagcaaggg
     1801 agtcgtccat gatgtccgaa catcggattc caatcttcat cgccggtgga ggcattctgt
     1861 gcctcctttt gcagctggtg ctcagcctgg ccgacagtcc agccagacga gcccagcacg
     1921 tcgcgcagaa agtcgccatc gagtcggtct atctccgatc cgaaaaacgc gccgagcggc
     1981 tggcgcagcg actaaaccaa gcacttgccg agggtgatca agccaagctg caagcggagc
     2041 tggatctggc gtcccaagcc gatccagacc tggtcgaaat tggcgtcgtc gatcacgatc
     2101 tgtcggtcgt tgcatcgtcg gaaaaaggcc gaatcggcaa gccacttggc gggcacgcag
     2161 agtcgctggt caaaaaggcc gtcgccgcga actttgcccg tcaagttgaa tcattgccgc
     2221 agaatcagcg tgtggttttg gcgctgccag tgacggggca gccgggctca agcggactgc
     2281 tttatctgga acttacgacg gcggaaatgg aagacgggct cggcaaagtg gtcacgacgg
     2341 tgcagccgca gcgagtgcca cagtggctga cgatcgggct tggcatcctt ggcgtcgggg
     2401 ttgcgcttgg gatgtggctg ctaaacgatc gcaagcgcga acggcagtcg atggtggttc
     2461 aagaagtcgc cgagcaaatg gcgatgggac actttgaaac ccgactgagc ccggcggaag
     2521 tgccgtcgtt tgcggcgatt gccgagcggc ttaacatgat ggccgaccaa gcggcgcttt
     2581 tgcagcagac gcagctcgaa ggcacggcgc acacgtcgcg gatggcgcag gaactggaag
     2641 acgcccagat tgtgcagcag acgctgatgc cggatgtgcg acggatttcg cgtggcgcgc
     2701 ttcagctttg tggggtgtat cgcagcgcca gcaaactttc gggagactgg tggcactact
     2761 atccgctcga tgagcaccgg acgatgctgg tcatggccga cgtggttggg cacggcattg
     2821 gatcggcggt cgtcggtgcc atggcatacg gctgcgccca gcagctccac caagagcaag
     2881 gccccaagct gcgccccgag cttctgcttt cacg
//
LOCUS       DAORGN010000337         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_22090_length_2871_cov_5.257457, whole genome shotgun sequence.
ACCESSION   DAORGN010000337 DAORGN010000000
VERSION     DAORGN010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22090_length_2871_cov_5.257457"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PKL17_21300"
     CDS             complement(<1..741)
                     /locus_tag="PKL17_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47334.1"
                     /translation="MTQTTRRTFTQSLHHAFAFPTPAGSRTFAFGSLLATVTGLFPAC
                     DPATAPSADDPRIDSIEQGVIAYGQADWVQLRGRRDTSQVQYYKEFWRDTSGCNSRFG
                     CRSMTVFIKLRVKPAASADLSKKKIGVVYREAGRTDPVTVVGNYFTTHGDGYEEWHVP
                     VKSDTFRGTFLFTAWYEDGAGGTFFDDNSGQRYAIQWQSSDWQTLAFDYANTTARFDS
                     TGVHGVLSFVVQDLDYDKVLKLVYSTDGW"
     gene            827..1393
                     /locus_tag="PKL17_21305"
     CDS             827..1393
                     /locus_tag="PKL17_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47335.1"
                     /translation="MRLRLAVVDESVSPLVESHESLTRQALLPDAVAKKLELLTGGWD
                     FVGEQDGMRLFVAQQVPLELRIGQLLLRPPSLEDAQLRALLTTGLTASQSLHQISDER
                     GRSLAGWPLRLLQGRVVNHGAWSGGDSEHRLVASYRFFEHSGEALLKVGNDSLLERCR
                     DDLLALLRSGWPQFSPPVGVATIAELWQ"
     gene            1474..1827
                     /locus_tag="PKL17_21310"
     CDS             1474..1827
                     /locus_tag="PKL17_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47336.1"
                     /translation="MFSKKHFLALSFLLLVPGCLLRANTVIDRFAGEFKCSRDRVTVE
                     RPDKNRPEFYKATGCNRRASYQCTGDYGEFCERIGQPETINPEGGALPSIPSPDTPAA
                     PTPPKDKPEAAPAAP"
     gene            complement(1863..2318)
                     /locus_tag="PKL17_21315"
     CDS             complement(1863..2318)
                     /locus_tag="PKL17_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47337.1"
                     /translation="MSRQLPWVHVELVPLYLQIRNLRRFVRDFCVGMSLRPEYAEHVA
                     MASAELLEVAAKGASAEWVRYDLRLLGTFTEVSVTGTATRKQRETLQQVVSEVSQGEA
                     QEAYARSLSQGEISGIGVAGLSRLRHEAAAELALSQNDEEVCLLIRVPI"
     gene            complement(2343..>2871)
                     /locus_tag="PKL17_21320"
     CDS             complement(2343..>2871)
                     /locus_tag="PKL17_21320"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNK47338.1"
                     /translation="QQLHQEQGPKLRPELLLSRLNQAIGSTMRGKFTMSCFAAIIDIK
                     ECSITFASGAHAFPLLFRHRETNKPFIPLVAAGSPLGSSDGAKFVANTQPFEPGDILI
                     CYTDGLIEAQNGNGDQFGDKRVRQTVQRGSNRNVEEICEMLIGEVSRFVGSASLEDDQ
                     TLVVARHGSPSYVGA"
BASE COUNT          534 a          833 c          894 g          610 t
ORIGIN      
        1 ccatccgtct gtcgaataga ccaatttgag cactttgtcg tagtcgagat cctgaaccac
       61 aaacgacagc acgccatgga cgcccgtcga atcgaagcgc gccgtggtgt ttgcgtagtc
      121 gaaagccagc gtctgccagt cgctcgattg ccactggatg gcgtaccgct gaccgctgtt
      181 gtcgtcgaaa aacgtgccgc cggcgccgtc ttcgtaccac gcggtaaaca ggaaagtgcc
      241 acggaatgtg tcacttttga ccgggacgtg ccattcctcg tagccatcgc cgtgggtcgt
      301 aaagtaattg ccgacgacgg tgacgggatc ggtgcgaccc gcttcgcgat agacgacgcc
      361 gattttcttt ttcgagaggt cggcagaagc agccggcttg acgcgcagct tgataaacac
      421 cgtcatcgat cggcagccaa agcggctgtt gcaacccgag gtgtcgcgcc aaaattcttt
      481 gtagtactga acttgcgagg tgtcgcgacg gcctcgaagc tggacccagt cggcctgacc
      541 gtaggcaatg acgccttgct cgatggaatc aatccgcggg tcgtcggcac ttggcgcggt
      601 ggccggatcg caagccggaa acagtcctgt caccgtcgcg agcagcgatc caaacgcgaa
      661 cgtgcggctc ccggctggcg tcggaaatgc aaaggcatgg tggagggact gcgtgaacgt
      721 gcggcgggtc gtctgtgtca tggcaagacc tcgaatgcga gcccgcggga cttgggctcg
      781 actgcgcgcg ttctatagca gcatttccgc tacaattcca aactgcatgc gcttacgtct
      841 cgctgtggtg gatgaatcgg tgtcgcctct cgtcgagtca cacgaatcac tgacgcggca
      901 agccctgctg cccgacgctg tggccaaaaa gctggagctt ctgacgggcg gctgggattt
      961 tgttggcgaa caagacggca tgcggctgtt tgtcgcgcaa caagttccgc tggagcttcg
     1021 catcgggcag cttttgcttc ggcctccgtc gctggaagac gcgcaactgc gggcgctgct
     1081 gacgacgggg ctgaccgctt cgcagtcgct gcaccagata tccgacgagc ggggtcgcag
     1141 cttggcgggc tggccactgc ggcttttgca ggggcgggtg gtcaatcacg gggcttggag
     1201 cggcggcgat agcgaacatc gcttggtcgc gagttatcgt ttttttgaac acagtggcga
     1261 ggcactgctc aaagtcggca acgattcact cctcgagcgc tgccgtgacg acttgctggc
     1321 gctgctgcgg agcggctggc cgcagttttc gccacccgtt ggggtcgcga cgattgcgga
     1381 actgtggcag tagtccgctg cgatgggcct tggctgagtc gggccgcaag cttcctcttt
     1441 cgtcgccttg gggtctttct ctatgatgag aaaatgtttt ctaaaaaaca tttcttggct
     1501 ctctcattcc ttcttctggt tcctggctgc ttgctgcgcg ccaacaccgt gatcgatcgc
     1561 tttgccggtg agttcaagtg cagccgcgac cgcgtcaccg tcgaacgccc tgacaaaaat
     1621 cggcccgagt tctacaaagc gacgggctgc aaccgccggg cgagctacca gtgcactggc
     1681 gattacggtg agttttgcga gcgcatcggc cagcccgaga cgatcaatcc agaaggcggc
     1741 gcgctgcctt cgatcccgtc tccagatacg cccgcggcac cgactccgcc caaagacaag
     1801 ccggaagccg ctcctgcagc gccgtaaatt gtgaattgaa ttactgaacc gattgcactg
     1861 gttcagatcg gaacgcggat cagcaggcag acttcttcgt cgttttggct gagcgcaagc
     1921 tctgccgcgg cttcatggcg cagacgactg agcccagcga cgccaattcc agagatttcc
     1981 ccttggctta agctgcgggc gtaggcctct tgcgcttccc cttggctgac ttccgacacc
     2041 acttgctgta gcgtttcgcg ctgttttctc gtcgctgtgc ccgttacgct gacttcggtg
     2101 aaagtgccga gcagccgcag atcgtagcgt acccattctg ccgacgctcc tttggccgcg
     2161 acttccagaa gttctgccga agccatcgcg acgtgctcgg cgtattccgg tcgcagcgac
     2221 atcccgacgc agaagtcgcg aacgaagcgc cgcagattgc gaatttgcaa atagagcggg
     2281 acaagttcga cgtgaaccca gggaagctgc ctagacattg cgactccgat gcgctgcttt
     2341 gactacgctc cgacatacga cggcgagccg tgccgtgcca caaccagtgt ctggtcgtct
     2401 tcgagcgagg ccgaaccgac gaagcgtgac acttcgccga tgagcatttc gcagatttct
     2461 tcgacgttgc ggttgctgcc gcgctgaacg gtctggcgga ctcgtttgtc gccgaattga
     2521 tcgccgttgc cgttttgggc ttcgatgaga ccgtcggtgt aacagatcag gatgtcgccc
     2581 ggttcaaacg gctgggtgtt ggcgacgaac ttggcgccgt cgcttgatcc aagcggtgaa
     2641 ccggcagcga cgagcgggat aaacggcttg ttggtttctc gatgacggaa cagaagcggg
     2701 aaggcgtggg caccgctggc gaacgtgatc gagcactctt tgatgtcgat gatggcggcg
     2761 aaacagctca tcgtgaactt gccgcgcatc gtcgagccga tcgcttggtt gagccgcgaa
     2821 agcagaagct cggggcgcag cttggggcct tgctcttggt ggagctgctg g
//
LOCUS       DAORGN010000338         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_23838_length_2747_cov_6.545691, whole genome shotgun sequence.
ACCESSION   DAORGN010000338 DAORGN010000000
VERSION     DAORGN010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23838_length_2747_cov_6.545691"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKL17_21325"
     CDS             <1..397
                     /locus_tag="PKL17_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47339.1"
                     /translation="NRGGFVDKLTAQPGWRCSLSDQKVSCLRYRPLQPGESSQVVAVT
                     VLSNEASTVDMPLVTTTATVASDGSTDPNPGDNTVTQTTELGVLRVAGGGLGCSTATG
                     GSTGGALGLLATTLLALGLRLRRRFAARR"
     gene            482..2374
                     /locus_tag="PKL17_21330"
     CDS             482..2374
                     /locus_tag="PKL17_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNK47340.1"
                     /translation="MRPLLQRSILAVLALAATQGAVSEAQAQTSQPNMGYQLNRYEPT
                     AAGEWSFAVEHPWFSSTRYFAAGLSLNYAHKPLVLGYKDSTGFTETQSLVSHQLIGHL
                     DLAGSFLDRVLVTMSLPVVMLSQGTLTDKNPNEGSFSVGDPRIGALARIYGQPNQNTW
                     SVSAGLDLWVPLRGISDTLPSIVSDQSVRVLPKAVFAGVWKKLLWSGSLGFLIRPEAQ
                     TLLPQEWTGIEAGRAASELQIGLRAAYYDQERRFAIGPELLLASALAGKDSFSRYGTS
                     LEALLGGHYNILNTVQVGAALGLGFVRQPGTPDVRGLLRVAYAPQTKKEPDGDGDGII
                     DKYDACPTEKGVASRDPKKHGCPVEPDRDSDGVPDKSDLCPDTPKGLAPDPDRLGCPL
                     PEKPQPEPDRDGDGVPDRNDQCPSEPQGATPDPGRLGCPAMDSDKDGVLDPVDQCLFE
                     PAGPSPDPARPGCPMADKDRDQIPDAEDACPDKAGAPHPDKKRHGCPSVVEIRGCQLV
                     ILKPIQFKPNSEVIDEKQSGKVLLAVADALKASPSIKRLRIEGHTDTQCPAKFRKNCK
                     SYNQDLSERRVVSVRKWLIKNGIEEGRLEGKAYGQDKPIAPNNPTGWGKNRRVEFHLP
                     DCTSGN"
     gene            2467..>2747
                     /locus_tag="PKL17_21335"
     CDS             2467..>2747
                     /locus_tag="PKL17_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNK47341.1"
                     /translation="MQRAFLVVVVAALGYFVDILDLFLFNVVRQPSLRDLGVSPADSL
                     FWGMRLLNAQMVGVLVGAFVFGTLGDRLGRLRALYGSILIYSVGTLLNG"
BASE COUNT          501 a          833 c          879 g          534 t
ORIGIN      
        1 gaatcgcggt ggtttcgtcg acaaattgac ggcgcagccc ggctggcgct gctctctgtc
       61 ggatcagaaa gtaagctgcc tgcgctatcg tccgcttcag ccgggagagt cgagccaagt
      121 ggtggctgtg acggtgctgt caaatgaggc gagcactgtc gacatgccgt tggtcacgac
      181 gaccgcaacg gtggcctcgg atggatcaac ggacccgaat ccgggcgaca acaccgtgac
      241 ccaaacgacg gagcttggcg tgctgagggt ggccggtggt ggtctgggct gttcgacggc
      301 cacgggtgga tcgacgggtg gcgcgctcgg acttttggcg acgacactgc tcgcgctggg
      361 tctgcggctg cgtcgccgat ttgcggcccg gcgctaggtg gaaaaacggc tcgacgaaac
      421 atgtatgtcg cgctgccgca gctgcggtgg cgctgccccg aacaggagat ggagggctgc
      481 tgtgcgacct cttttgcaac gctcgatttt ggctgtgctc gcgctcgctg cgacgcaagg
      541 tgcggtaagc gaagcgcagg ctcagacttc ccagcccaac atgggctacc agctcaatcg
      601 gtatgagccg accgccgccg gtgagtggtc gtttgcggtg gaacatccgt ggttttcgtc
      661 gacgcgctac tttgccgcgg ggctttcgct aaactatgcg cacaagccgc ttgtcctcgg
      721 gtacaaggac agcaccggct ttaccgaaac gcagtcgctc gtctcgcacc agcttatcgg
      781 gcatctcgat ctggccggtt cgtttctgga ccgtgtgctt gtgacgatgt cgctgccggt
      841 ggtgatgctc agccaaggca ccctgaccga caaaaatccg aatgaaggga gcttttccgt
      901 cggtgatccg cggatcggtg cgctggctcg catctacggc cagccgaatc agaacacctg
      961 gtcggtgagc gcggggcttg atctgtgggt tccgctgcgc ggcatttccg acacactgcc
     1021 gtcgatcgtg agcgatcaat cggtgcgcgt gctgcccaaa gcggtgttcg cgggcgtgtg
     1081 gaagaagctc ctgtggtcgg gcagcctcgg ttttctgatt cggcccgagg cgcagacgct
     1141 gctgccgcag gagtggaccg ggatcgaggc tggtcgcgct gcgtcggaac tacagattgg
     1201 actgcgcgcc gcgtactacg accaagagcg acggttcgcc atcggcccgg agctgctttt
     1261 ggcgtcggca ctcgcgggca aagatagctt ttcgcgttat ggcaccagcc tcgaagcgct
     1321 gctcggtgga cattacaaca tcttaaatac cgtgcaggtc ggtgcagcgc tgggccttgg
     1381 ttttgttcgt caaccgggga cgcccgacgt gcgcggactt ttgcgggttg cctacgcccc
     1441 gcagacgaaa aaagaacctg acggagacgg cgacggaatc atcgacaagt atgatgcttg
     1501 tccgacggaa aaaggcgtcg caagccgcga tccgaaaaag cacggctgcc cggtcgaacc
     1561 cgatcgagac agcgacggag tgcctgacaa gtccgacctc tgtcctgata cgccaaaggg
     1621 tctggcgcca gatcccgatc ggctgggttg tccgctgccc gaaaagccgc agcccgagcc
     1681 cgaccgtgac ggcgatggtg tgcccgatcg caacgatcaa tgtccgtcgg aaccgcaggg
     1741 cgcaacgcca gatccgggtc gactgggctg cccggccatg gacagcgaca aagacggcgt
     1801 gctcgatccg gtggatcagt gtctgttcga gccggcgggt ccaagccccg atccagcgcg
     1861 tccaggctgc ccgatggccg acaaagatcg cgatcagatc ccggatgccg aagatgcatg
     1921 cccggacaaa gccggcgcgc cgcatcctga caaaaagcgt cacggctgcc caagcgtcgt
     1981 cgagatccgc ggctgccaac tggtgatttt gaagccgatc cagtttaagc caaacagcga
     2041 agtcatcgac gaaaagcaga gcggtaaggt ccttctcgcc gtcgctgatg cactcaaagc
     2101 gtcgccgtcc atcaagcggc tgcgcatcga gggccacacc gacacgcagt gcccggccaa
     2161 gttccgcaaa aactgcaaga gctacaacca ggatctgtcg gaacgtcgcg tcgtcagcgt
     2221 ccgcaagtgg cttatcaaaa acggcatcga ggaaggtcgt ctagagggca aagcctacgg
     2281 tcaggacaag ccgatcgccc cgaacaaccc gactggctgg ggcaaaaacc gccgtgtcga
     2341 gttccatctg cccgactgca ccagcggcaa ctagacccgc cttctgtcgg tagcccaccg
     2401 cgctgccgac gtgtcttttg cgcttctttc ttcccgccat cgcccgcttt gccgatactg
     2461 accgccatgc agcgggcctt tcttgtcgtc gttgtcgccg cgcttggcta cttcgtcgac
     2521 atcctcgact tgtttctgtt taacgtcgtg cggcagccga gtctgcgcga cctgggcgtg
     2581 tccccggcgg attcgctgtt ttggggcatg cggctgctca atgcccagat ggtcggcgtg
     2641 ctcgtcggtg cgttcgtttt tggcacactt ggcgaccggc tcggcaggct gcgcgcgctg
     2701 tacggttcga ttctgatcta ttccgtcggt acgctgctca acgggtt
//
LOCUS       DAORGN010000339         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_24097_length_2731_cov_4.221226, whole genome shotgun sequence.
ACCESSION   DAORGN010000339 DAORGN010000000
VERSION     DAORGN010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24097_length_2731_cov_4.221226"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKL17_21340"
     CDS             <1..863
                     /locus_tag="PKL17_21340"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNK47342.1"
                     /translation="VPEINWAMMVGCLALVLAFRKEGSSGLAAAYGIAVTGTMSITSI
                     LFAVVARERWGWSVVKIGSLVGCFLLIDLSFFGANLAKVADGGWIPLAVAIGIFIVMT
                     TWKAGRAALEQFVQSASLPMDLFLGDLQERNLPRVKGTAVFMTGNPEGAPVVLLHHIK
                     HNKSLHEQVVLLSVLTDRVPEVPPSKRVSVKELGQGFFQVIAHYGFMQSPNVPDVIRR
                     CKDAGLKTAEGDISYFLGRETLLTTGKSGLARWRKMLFGFLSRNARPATSFFGIPPNR
                     VVEMGAQIEL"
     gene            complement(935..1807)
                     /locus_tag="PKL17_21345"
     CDS             complement(935..1807)
                     /locus_tag="PKL17_21345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNK47343.1"
                     /translation="MLTIALLGLLWSILRISVPYVMATLGGTVCEKAGVINIALEGIL
                     LLSALGTALGAPHGALVALVCGLLAGVATALLYGLLVVVFRGDQIVCGVAIFMLADGL
                     SRYLLKVVFGSTSNSPRLDALVGHGPLWLLATTLLATLLLHGMIHHSVFGLRLRAVGD
                     KPAAAQALSVSVLRVRLWAVLLSGLLTAVGGVYLVFEQRQFVALMSGGRGFLALAAMI
                     FGGWRPLWGAAAALLFATAEATGIFLQARGLLIPNWLLQCVPYVITLLSLMLRGLVGQ
                     KHSSAPLALGIPRP"
     gene            complement(1810..>2731)
                     /locus_tag="PKL17_21350"
     CDS             complement(1810..>2731)
                     /locus_tag="PKL17_21350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47344.1"
                     /translation="IVGALAAALCGAKLPPQTPSVVAVTLCLLCAFSAGALLGALVGI
                     CKWLCRAHEVIVTILGNFIVRAVMVGLGSVLFLKESVHTAPIIEPARLPRLSTLFPQL
                     HGSAVNATLFVVIGLALAVEVMLRSTRFGFALRVVGQNPDAAEAAGLSIGKVRTLALL
                     LSGGLAGLGGANFVLGYKYYYEDGFSSGIGYLGIAVAVLAHNRPLWLLLSALFFGTLA
                     QGGLAVNAMVPRELIDVLSAVVILAVATATAEVSRLLPRFSAASRPNEPAAPSQTPPG
                     EQPDTAPETKITPHLLSETQAASAAAWETT"
BASE COUNT          574 a          858 c          842 g          457 t
ORIGIN      
        1 acgtgcccga gatcaactgg gcgatgatgg tcggctgtct ggcgctggtg ctggcgttcc
       61 gcaaggaagg atcgtcggga cttgcggcgg cctacgggat cgcagtgact ggcacgatgt
      121 cgattacctc gattttgttc gcggtggttg cgcgtgaacg ctggggctgg agcgtggtca
      181 aaatcggatc gctcgtcggc tgttttttgc tgatcgatct gtcgtttttt ggcgccaact
      241 tggcgaaagt cgccgacgga ggctggattc cgctggcggt cgcaatcggc atcttcatcg
      301 tcatgacgac gtggaaagcg ggccgggcgg cgctcgaaca gtttgtgcag agcgcgagcc
      361 tgccgatgga tctgttcctg ggtgacttgc aagagcgcaa cctgccgcgg gtgaagggga
      421 ccgccgtgtt tatgacgggc aaccccgaag gcgcgccggt ggtgctgctg catcacatca
      481 agcacaacaa gtcgctccat gaacaggtcg tgctgttgtc ggtgctgacc gaccgggtgc
      541 ccgaagtgcc gccgtcgaag cgcgtcagcg tcaaggagct agggcagggg tttttccagg
      601 tgattgccca ctacggcttc atgcagagcc cgaacgtgcc ggatgtgatt cgtcgctgca
      661 aagatgcagg gctcaagacc gccgagggag acatcagcta ttttctcggg cgggagacgc
      721 ttttgacgac cggcaagagt ggtctcgctc gctggcgcaa gatgctgttt ggctttctgt
      781 cacgcaatgc ccgccccgcg acgtcgttct ttgggattcc gccaaaccga gtcgttgaaa
      841 tgggcgcgca aatcgagctg tgacaccctg cccaagcgac actgcgtagc attcgtgcgg
      901 ctggggttcg gtcgcttgcg cgcttccagc ggtcttatgg acgagggatg ccgagtgcga
      961 gcggagcgct gctgtgcttt tgcccaacca ggccccgcag catcagcgaa agcagggtga
     1021 tgacgtaggg cacgcactgg agcagccagt tgggaatcag cagtccgcgc gcttgcagga
     1081 agattcccgt ggcttctgcc gtcgcgaaca gcagcgccgc tgccgcaccc cacagcgggc
     1141 gccagccgcc aaaaatcatc gcagccagcg caagaaagcc acgcccaccc gacatcagcg
     1201 cgacaaactg ccgctgctcg aagaccagat agacgcctcc cactgcggtc aaaagtcccg
     1261 acagcagcac cgcccaaagc cgcacgcgca gcaccgacac cgacagcgct tgcgccgcgg
     1321 ctggtttgtc acccaccgcg cgcagccgca gcccaaacac cgagtgatgg atcattccgt
     1381 gcaaaagcag cgtcgccaag agcgtcgtcg cgagcagcca caaaggaccg tgtcccacca
     1441 gcgcatcgag ccgcggcgag tttgaagtcg aaccaaacac cactttcagc aggtagcgcg
     1501 aaagtccgtc ggccagcatg aaaatggcga cgccacagac aatttggtcg ccgcgaaaca
     1561 ccaccaccag cagtccatac agcagtgccg tcgctacacc cgccaaaagc ccgcagacaa
     1621 gtgcgaccag cgcgccatgc ggagctccaa gcgcggtgcc cagcgccgac agaagcagga
     1681 tgccctctag cgcaatgttg atgacaccgg ctttttcgca gaccgtgcca ccgagcgttg
     1741 ccatcacata cggcaccgag atgcgaagaa tcgaccacag caggccaagc agcgcgatcg
     1801 tcagcatgct taagtcgtct cccaagctgc ggctgacgct gcctgcgttt cgctcagcag
     1861 atgcggtgtg atcttcgttt ctggcgcggt gtccggctgc tctcctggcg gtgtctgcga
     1921 tggggcagcg ggctcattgg gccgagaagc ggcagaaaac cggggcagca atcgactgac
     1981 ttcggcggtt gcggtggcta ctgcaaggat tacgaccgcg ctgagcacgt cgataagttc
     2041 ccgcggaacc attgcgttta ccgccagtcc gccttgggcc agcgttccaa agaacagcgc
     2101 cgacaaaagc agccacagcg gtcggttgtg ggccaacacc gcgacggcaa ttccgagata
     2161 gccaattccc gacgaaaaac catcctcgta gtagtacttg tacccgagaa caaagttcgc
     2221 accgccaagc cccgcgagtc cgcccgaaag cagaagtgcc aaggtgcgga ctttgccgat
     2281 ggaaagaccc gccgcttctg ccgcatctgg attctgtcca acgactcgca gcgcaaagcc
     2341 aaagcgcgtc gagcggagca tcacttcgac ggccagcgcc agaccgatga ccacgaacag
     2401 cgtcgcattg accgccgagc cgtggagctg cggaaacagc gtcgacagcc tcggcagccg
     2461 ggccggttcg atgatcggtg ccgtgtggac cgattccttc agaaacagca cgctgcccag
     2521 gcccaccatc acggcgcgaa cgatgaagtt gcccaagatg gtgacgatga cctcgtgcgc
     2581 gcgacacagc cacttgcaaa tcccgaccag ggccccgagc agcgccccag ccgaaaacgc
     2641 gcacaaaaga cacagcgtca ctgcgaccac actcggggtt tgtggcggta gcttggcgcc
     2701 acacagcgcc gccgcaagtg caccgacgat t
//
LOCUS       DAORGN010000340         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_25055_length_2674_cov_7.192822, whole genome shotgun sequence.
ACCESSION   DAORGN010000340 DAORGN010000000
VERSION     DAORGN010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25055_length_2674_cov_7.192822"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..491)
                     /locus_tag="PKL17_21355"
     CDS             complement(69..491)
                     /locus_tag="PKL17_21355"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47345.1"
                     /translation="MFVGKVVGTVWSTVKWPGVSGHKLLLVRPYHLGDLLAHPAATVS
                     ESGSEPSKSAAPPAHVDAEAVVCIDLLDAGVGDDVVVAFGHAARVAADPSLLEPSFHQ
                     SSSGHAAALPPKVSPPVTPIDAAVVAVVDGFHIVRPGT"
     gene            complement(496..822)
                     /locus_tag="PKL17_21360"
     CDS             complement(496..822)
                     /locus_tag="PKL17_21360"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47346.1"
                     /translation="MKRGVLIGEVWATRKAAGLAGKSLKLVLESPSLSEPGALYAPIT
                     VAIDTLDGKLGQPVLVAYGSGARNVMAKGASNRAVLADAAVAVLLDGTEPSAANDDPA
                     QAPKKE"
     gene            complement(819..1238)
                     /locus_tag="PKL17_21365"
     CDS             complement(819..1238)
                     /locus_tag="PKL17_21365"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47347.1"
                     /translation="MILGRVVGELWAARRHPGLSGRKLLLIEPHYWYCPPFEVAHLVA
                     VDTLGAGPGDDVIVCLGEPARQSLCEQPAGRGSVPGYASAAMLPVEAAVMAIVDRVDI
                     DKQPAPFTGRPLRTVLPSTFAESIETGRTPTDRELSR"
     gene            complement(1235..1510)
                     /locus_tag="PKL17_21370"
     CDS             complement(1235..1510)
                     /locus_tag="PKL17_21370"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNK47348.1"
                     /translation="MLLARVVLPLWGARRADSLTGFKLLELETPGGSRLCALDALGAG
                     PGEWVIVAHGSRVRDLTCGPDVADKDIIIAIVDGFEREDGQAEGLSP"
     gene            complement(1515..1874)
                     /locus_tag="PKL17_21375"
     CDS             complement(1515..1874)
                     /locus_tag="PKL17_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47349.1"
                     /translation="MKKSPLAQVKETYGSKEKLVDALVGLPEGVLDRSDDKDAWKQQL
                     LSAANAKLLRLHTVGSQVAKRFGSKEKLVDALLGLQKRGKDQDFRSKLVTLPIGKLYD
                     RYISVERAQKRAQASKK"
     gene            complement(2084..>2674)
                     /gene="metG"
                     /locus_tag="PKL17_21380"
     CDS             complement(2084..>2674)
                     /gene="metG"
                     /locus_tag="PKL17_21380"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNK47350.1"
                     /translation="RRQLGLLNPEQPSWPSQWGALPDGTVVRKGATLFPRIDPDRQKE
                     LLGKWRAARLAAEEKAQASAPKPQPTQASTSGAATEAKHPAESLPEIGIDDFKKIELR
                     IAKVLTAERVPKKDRLLKVELDLGPLGKRQVIAGIAELCTPESLVGKNVVLLCNLKPA
                     KIGGQVSSGMILALGDDKLLGLATGDREVPPGTALR"
BASE COUNT          490 a          862 c          774 g          548 t
ORIGIN      
        1 ccaggtgcag acagcggcgg cgaaagagac tcgcatagcg aaacggtacc gcttgtcgcg
       61 tacatgagtc aagtacccgg tcgtactatg tgaaagccat cgacgactgc caccaccgcc
      121 gcatcgattg gcgtaaccgg agggctcacc ttgggcggaa gcgccgctgc atgtcccgag
      181 gaagattgat gaaatgatgg ttcaagcagc gacgggtccg cggcgactct ggcggcatgt
      241 ccgaacgcga caaccacgtc atcacctacg ccggcatcca gcaagtcgat gcacaccact
      301 gcttccgcat cgacatgggc cggtggggca gccgacttgc ttggctccga tcccgattca
      361 gacaccgtag ctgctgggtg cgccaggagg tcgcccaaat gatacggcct cacgagcagc
      421 agcttgtgac cgctcacgcc gggccacttt acggtcgacc aaacggttcc aaccaccttg
      481 ccaacgaaca taccgctact ccttttttgg cgcctgtgca gggtcgtcgt ttgcggcaga
      541 aggttcagtg ccatctagca acacggccac cgcggcgtca gcaagaaccg ctcgattgct
      601 ggcccccttt gccatcacgt tgcgcgcacc cgatccgtaa gcgacaagga ctggctgtcc
      661 aagcttgccg tcgagcgtgt cgatggccac ggtgatggga gcgtacagcg cgccaggttc
      721 cgacaggctg ggcgattcaa gcaccagctt gaggcttttg cctgcaagac ctgccgcttt
      781 gcgcgttgcc cacacttcac cgatgagaac gccgcgtttc atcgcgaaag ctcccggtcg
      841 gttggtgtcc tgcctgtttc gatcgattcg gcaaacgtcg acggcagaac cgtccgaagc
      901 gggcgtcccg tgaagggtgc cggctgtttg tcgatgtcga cccgatcgac gatggccatc
      961 acggctgctt ccaccggcaa catcgccgcg cttgcgtagc ccggtacact gccgcgacct
     1021 gccggctgtt cgcaaagtga ctgacgggct ggttcaccca aacagacgat gacatcgtct
     1081 ccggggccag cgccaagggt atcgacagcg acgaggtgtg caacctcaaa cggtggacaa
     1141 taccagtagt gcggttcaat cagcagcagc ttgcgcccag acagacctgg atgacgccgc
     1201 gctgcccaaa gctcgcccac gactcgacca agaatcatgg cgaaagtcct tcggcctgcc
     1261 cgtcttcgcg ttcgaaccca tcaacgatgg cgatgatgat gtccttgtcc gcgacgtccg
     1321 gcccgcacgt caggtcgcgt acccgagagc catgggcgac aatcacccat tctcccggtc
     1381 cggcgccgag tgcatctagt gcacacagtc gactgcctcc cggcgtttca agctctagta
     1441 gcttgaatcc cgtgaggctg tctgcccgcc tcgcccccca cagtggtaaa acgacgcggg
     1501 ccagtagcac ggaactactt cttgctggcc tgggcgcgct tctgggcgcg ctcgaccgaa
     1561 atgtaacgat cgtacagctt gccaatcggc agcgtgacga gcttgctgcg gaagtcctgg
     1621 tccttgccgc gcttttgcag accgagcagc gcgtcgacca gtttttcctt gctgccaaag
     1681 cgcttggcaa cctggcttcc gacggtgtgc agccgcagaa gcttcgcgtt cgcagcagac
     1741 agaagctgct gcttccaggc gtccttgtcg tccgagcgat cgagaacacc ttcgggaagc
     1801 ccgacgagtg cgtcgaccag cttctccttg ctgccatacg tttccttcac ctgagcgagc
     1861 ggtgactttt tcattgtctt tttgtgctcc taaactttgc gtgcgcgacg ggaatttgga
     1921 tctctgcgcg aaacaatggg cttttgacag ctcaaaccga cgcgccgtga gtgccgggac
     1981 tttatcaccg tcccgtttca gtcctcaagc atgttttcca cagggatttt ggctggctgg
     2041 caaatcctgc caaagctctt gcgcaggtcc agtgcagatg gtcttagcgc agcgcggtgc
     2101 cgggtggaac ttcgcggtcg ccggtcgcca gcccaagcag tttgtcgtct ccaagtgcca
     2161 ggatcatccc cgacgagacc tgtcccccga ttttggccgg cttcaagttg cacaaaagca
     2221 ccacgttttt gccgaccagg ctttcgggcg tacacagctc ggcgatgccc gcgatcacct
     2281 gccgcttgcc aagcggtccc aggtccagct cgactttgag cagccgatct tttttcggaa
     2341 cgcgctctgc cgtgagcact ttggcgatgc gcagctcgat tttcttaaag tcgtcgatgc
     2401 caatctccgg cagcgactcg gcgggatgct tggcttccgt tgctgctccc gatgtcgaag
     2461 cttgggtcgg ctggggtttg ggagcgctcg cctgcgcttt ttcttccgcc gcgagccggg
     2521 ccgcccgcca cttcccgagc agttcctttt gccgatccgg atcaatgcgt ggaaacagcg
     2581 tcgcgccttt gcgcaccacc gttccatctg gcagcgcgcc ccattgcgac ggccacgacg
     2641 gctgttccgg gttcaaaaga ccgagctgcc gccg
//
LOCUS       DAORGN010000341         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_26070_length_2616_cov_5.686451, whole genome shotgun sequence.
ACCESSION   DAORGN010000341 DAORGN010000000
VERSION     DAORGN010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26070_length_2616_cov_5.686451"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKL17_21385"
     CDS             <1..643
                     /locus_tag="PKL17_21385"
                     /inference="COORDINATES: protein motif:HMM:NF025594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HNK47351.1"
                     /translation="FKMMGIDIGGRMTVVRLADGTLLVVSPVALTPEERAELDRLGAV
                     RHIVAPNLMHHLYVGDLKKGYPDAKLYVPPGLTDKRKDLAPDGVLSDAPPAGLQGDFE
                     QHLVAGMPGLQEVVFFHKKSRTLIQTDLAFNLTQMPSLIGRILFRLNGALGGVRATKV
                     LRSLIKDRAATRASIEKILAWDFDRMIVTHGDVVRQGAKAELQAAYQFLLSKS"
     gene            711..1739
                     /locus_tag="PKL17_21390"
     CDS             711..1739
                     /locus_tag="PKL17_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006915078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNK47352.1"
                     /translation="MSVSTTPVISLIRGDGIGPEISEAVVKMLDAAGAKLSYEELPAG
                     EGALRLHQDPLPEITIQSISKNKVALKGPLSTPKGSGFRSVNVALRQHFDLYANVRPA
                     LSFPGVPSRYKDVDIVTVRENTEDIYAGIEHFVGPGRSAAESIAVITRFGSERIVRYA
                     FEYARRHKRKKVSIIHKANILKMSNGLFLEVAKAVASEYPDIQHDDVIVDAACMKLVL
                     FPERFDVIVTMNLFGDILSDLIAGLVGGLGVSPGANLGEKAAIFEAVHGSAPDIAGMG
                     IANPTALALAAEMMLHHLGQSEAAGRLRRGLLTGLADERLRTKDLGGQANTKTFTDGV
                     CAAIGHGG"
     gene            1761..2615
                     /locus_tag="PKL17_21395"
     CDS             1761..2615
                     /locus_tag="PKL17_21395"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNK47353.1"
                     /translation="MLKLHVLGVGDAFTERYHNACLCVEEPQSGTRLLLDCPPALPRV
                     LADHRERTLSDLAVHNIDHVLLTHLHGDHCGGLEAYLFMRRFLVRRRPFLYGAKEVLD
                     PLWDTRLRGGMAHLLPCVPEYSQSEVDACLASPSYLRPETDEPAQRALKTLPSVDKEL
                     SLADYAHRVDLAPRTEIGPLVIERRFTRHHIPTTALRISLVGQSSPLLGYSADTAFDP
                     DLIAWLSPSQLVVHETNFGVHTPLHALMLLPDSLREKLRLIHYPDLLDREHSPILCLK
                     QGTILTLA"
BASE COUNT          494 a          776 c          800 g          546 t
ORIGIN      
        1 gttcaaaatg atgggcattg atatcggggg acggatgacg gtggttaggc ttgcggacgg
       61 gacgctgctc gtggtgtcgc cggttgcact gacccccgag gagcgggccg aattggatcg
      121 tctgggcgcg gtgcggcaca tcgtcgcgcc aaaccttatg catcacctgt acgtcgggga
      181 tctgaaaaaa ggctatcccg atgccaagct gtatgtgccg cccggtctga ccgacaagcg
      241 caaggatctt gcgcccgacg gtgtgctgtc ggatgcgccg ccagcgggtc tacaaggtga
      301 tttcgagcag cacttggtgg cggggatgcc ggggctacag gaagtggtgt ttttccacaa
      361 gaagagccgc acgctgatcc agaccgacct cgcgttcaat ttgacgcaga tgccgtcgct
      421 gattgggcgg attctgtttc ggctgaacgg ggcgctcggt ggtgtgcggg cgaccaaagt
      481 gctgcgcagc ttgatcaaag accgggcggc gacccgcgct tcgatcgaga aaatcttggc
      541 atgggacttc gaccgcatga tagtcacgca cggcgacgtg gtccggcaag gtgccaaggc
      601 ggaactacag gcggcgtacc agtttttgct cagcaaaagc taaggcgcgc ggcagctttt
      661 ctccccgtgg accgcgccgc atcttacgca agaaagaagg ctctacatcc atgtccgttt
      721 cgacgactcc tgtcatctcg ctcattcggg gcgatggaat tggtccagaa atcagcgaag
      781 ccgtggtcaa aatgctcgac gcggcgggcg ccaagctttc gtatgaagaa ctgccggcgg
      841 gcgaaggcgc gcttaggctt caccaagacc cgctgcccga aatcaccatc cagtcgatca
      901 gcaaaaacaa agtcgcgctc aagggtccgc tgtcgacgcc aaagggctcg ggctttcgtt
      961 cggtgaacgt cgctttgcgg caacactttg atctgtacgc caatgtccgt ccggccctga
     1021 gctttcccgg tgtgccgtcg cgttacaaag acgtcgacat cgtgacggtg cgtgaaaaca
     1081 ccgaagacat ctacgctggc atcgaacact tcgtcgggcc gggtcgctct gccgctgaat
     1141 cgatcgccgt catcacccgc ttcggttccg agcgcatcgt ccgctacgct ttcgaatacg
     1201 cccgtcgcca caagcgcaaa aaagtgtcga ttatccacaa ggccaacatc ctgaagatgt
     1261 ccaacggcct gtttttggaa gtcgccaagg ccgtcgcatc ggaatacccg gacattcagc
     1321 acgacgatgt cattgtcgac gccgcctgca tgaagttggt gctattcccc gaacgcttcg
     1381 acgtcattgt gacgatgaac ttgtttggcg acattctgtc ggacttgata gcgggtctgg
     1441 tcggtggctt gggcgtgtcg ccgggcgcga acctgggcga aaaagcggcg atttttgaag
     1501 ccgtccacgg atcggcgccg gacattgcgg gaatggggat tgccaatccg acggcgctgg
     1561 cgctggcggc agaaatgatg ctgcaccacc tgggtcagtc ggaagcggcg gggcggctgc
     1621 gtcgcgggct cttgactggg ctcgctgacg aaaggctgcg caccaaagac ctcggcggac
     1681 aggccaacac caagacgttc accgacggtg tctgcgcggc gattggccac ggcggctagt
     1741 ttcgtcgcgg ggggatttcc atgctcaagc ttcatgtgct cggcgtcggt gatgccttca
     1801 ccgagcgcta ccacaacgcc tgtctgtgtg tcgaagagcc gcagtcggga acccgtcttt
     1861 tgttagattg tccgccggcg ctgccgcgtg tgcttgccga ccatcgcgag cgaaccctga
     1921 gcgacctggc cgtccacaac atcgatcatg tgcttttgac ccacctgcac ggggatcact
     1981 gcggcggact cgaagcgtac ttgtttatgc ggcgcttttt ggtgcgtcga cggcccttcc
     2041 tctacggtgc caaagaagtg ctcgacccac tgtgggatac gcgactgcgc ggcggcatgg
     2101 cgcatcttct gccgtgtgtg cccgagtatt cgcagagcga agtcgatgcg tgtctggctt
     2161 cgcccagtta tctgcgtccc gaaaccgatg aaccggcgca gcgggcgctc aaaacgcttc
     2221 cgtccgtcga caaagagctg tcgctcgccg actatgctca tcgcgtcgat ttggccccgc
     2281 gcacggaaat cggtccgctt gtcatcgagc gtcgctttac ccgtcaccac attccgacga
     2341 cggcactgcg catttcactt gtcggacagt cgtcgccgct tttggggtac agcgccgata
     2401 cggcgttcga tcccgacttg attgcgtggc tttcaccgtc gcagcttgtg gttcacgaaa
     2461 cgaacttcgg ggttcatacg ccgctgcatg cgctgatgct gttgcccgat tcgctgcggg
     2521 aaaagctgcg gctgattcac tatcccgacc tgctcgaccg cgaacactcc ccaattctgt
     2581 gcctcaaaca aggcaccatt cttacgctgg catagc
//
LOCUS       DAORGN010000342         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_26145_length_2612_cov_5.480641, whole genome shotgun sequence.
ACCESSION   DAORGN010000342 DAORGN010000000
VERSION     DAORGN010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26145_length_2612_cov_5.480641"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PKL17_21400"
     CDS             complement(<1..268)
                     /locus_tag="PKL17_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47354.1"
                     /translation="MSESRAVGDDTLLPGSQQATSEKRKLAADELPRGSSIGRYLIIE
                     KLGSGGMGVVYAAYDPDLNRKVAIKLLRSQPGAMASQGRARLLRE"
     gene            365..2302
                     /locus_tag="PKL17_21405"
     CDS             365..2302
                     /locus_tag="PKL17_21405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNK47355.1"
                     /translation="MRPFARWPLRFLRRLVIVLVVLMIAAGPSAAADDAIVLRVRGKS
                     QLKLRQVSRRPDGKNAFTVQFSLELSDGVSGDKSDSGDDRSFADRPILAQLRGPAGTL
                     YLPTKRTGDDGLVTFEQSAVEPATYSLLAEYRGDDLRDSVREVFTIDVGRQPTQLRLM
                     APARIRLSDELPLRIALTSDGAPLDGTVELSIGRKQETVTLSQGYGQHKLRAQALGKS
                     GDKLSIVARYKGTRQFAPSETKEELLLVSQATVQLQLIGATHEIPQGNALTALGLVRD
                     EQGPLAGEVVELEALTDETIPALDPASSKRSIGQAQTDSQGRFDIRIAKLLLPTGPVL
                     LSAQVFPRRGHILPGRSPEVALQVLPPEPISVLYFVLPLAVTLLLWVGFVLGRRVVAW
                     LRATWAARRLQQQSSTSKSTEAAAITHSTLDLSTGEPGVKLSPNRRLASLRRAADFAM
                     DGQVVDATFGIPVAATLSMVLDGLSQAEPGRTLTTEADGSFATPPLPQGRYQMRISAP
                     GYLPQQFSATVPHKGEYRQIAVRLEPLRVRLLAEWRRVAEGLGDDKVATQTPRELHDL
                     LTFAKSRRLSEPIQQKLGELTQLVENAYYSPRICTPEMLVSAAQLVDAILASETAASK
                     SPAPDLRAPSAPRPLINQPRA"
     gene            2317..>2612
                     /locus_tag="PKL17_21410"
     CDS             2317..>2612
                     /locus_tag="PKL17_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNK47356.1"
                     /translation="MRVALVSDIHANEVALRAVFRHIDNLGADITICLGDVATLGPHP
                     REVLNILQERKCPCIMGNHDEFLLDRQLIERYTEAPIIKQAVDWCRDELSTQDL"
BASE COUNT          531 a          824 c          771 g          486 t
ORIGIN      
        1 cttcacgaag cagccgcgcc cgaccctgcg acgccatcgc gcctggctga ctacgcaaca
       61 gcttgatcgc aaccttgcgg tttaggtcgg gatcgtaggc cgcgtagacg acgcccatgc
      121 cgccgctgcc cagcttttcg atgatgagat agcgcccaat ggacgaaccc cgtggcagct
      181 catccgctgc gagcttgcgc ttttcgctcg ttgcttgctg cgatccgggg aggagcgtgt
      241 catccccaac cgcccgactt tcagacatct agaagccaaa atgtaccagg ctcggcccgg
      301 cggccccagc ggaacccgcc aaacagcacg cagcgacgca gcttgcgtgc gtatactgct
      361 aaccatgcga ccttttgcac gatggcctct gcgttttttg cgccggctgg tcatcgtgct
      421 cgtcgtgctg atgattgccg ctggcccaag cgcggctgcc gacgacgcga tcgttctgag
      481 ggtgcgcggc aaaagccagc taaagctccg ccaggtaagc cgccgccccg acggaaaaaa
      541 cgcattcaca gtccagtttt cgcttgagct tagcgatgga gtcagcggcg acaaatcgga
      601 cagtggcgac gatcgatcgt ttgccgatcg accgattttg gcccagctac gcggccccgc
      661 cggcacgttg tatctgccga ccaagcgcac aggcgacgac ggacttgtga catttgagca
      721 aagcgccgtc gaaccagcca cgtacagcct gcttgccgaa taccgtggcg atgatctgcg
      781 cgactcggtt cgtgaagtgt tcaccatcga cgtaggcagg cagccgacgc agcttcgctt
      841 gatggcgccg gcgcggattc ggctttccga cgagcttccg ctgcgaattg ccttgaccag
      901 cgacggcgca ccgctcgatg gaaccgtcga gctttcgatc ggtcgcaagc aagaaaccgt
      961 gacgctcagc cagggctacg gccaacacaa gctacgcgcc caagcgctgg gcaaatcggg
     1021 tgacaagctg tctatcgtcg ccagatacaa aggcacacgg cagtttgcgc cgtcggaaac
     1081 caaagaagag ctgctgctgg tcagtcaagc cacggttcag cttcagctca tcggtgcgac
     1141 gcacgaaatt ccgcagggca atgcgctgac ggcgttgggg cttgtccgcg acgaacaagg
     1201 accgctggcc ggcgaagtgg tcgaactgga agcgctcacc gacgaaacca ttcccgcgct
     1261 agaccccgcc agcagcaagc gcagcatcgg gcaagcccag accgacagcc aaggacgctt
     1321 cgacattcgc atcgccaagc tgcttctacc gaccgggcct gtgctgcttt cggcccaagt
     1381 gtttccgcga cgcgggcaca ttcttccggg tcgctcgccg gaagtcgcgc tacaggtctt
     1441 gccccccgag ccgatctccg tcctctattt tgttttgccg ctcgcggtga cgctgcttct
     1501 gtgggtcggc tttgtgctgg gacgacgtgt tgtcgcgtgg ctgcgggcaa catgggcagc
     1561 gcggcgtttg cagcaacagt cgtcgacgtc aaaatcaacc gaggcggcag caatcaccca
     1621 cagcacgctg gacctaagta caggtgaacc gggcgtcaag ctgtcgccca atcgccgtct
     1681 ggccagcctg cgccgtgccg ctgactttgc gatggatggc caggtggttg atgccacgtt
     1741 cggaattccc gtcgctgcaa ccctttcgat ggtgctcgac gggctttcgc aggccgaacc
     1801 tgggcggaca ctgaccacgg aagcagatgg cagctttgcg acgccgccgc tgccacaagg
     1861 tcgctaccaa atgcgcattt ccgcgccagg ttatctgccg cagcagtttt ccgcgacggt
     1921 gccgcacaaa ggggaatatc gccaaattgc ggtgcggctc gaacccttgc gggtgcgact
     1981 tttggccgag tggcgtcgcg tggccgaagg gctcggcgac gacaaagtgg cgacgcagac
     2041 cccacgagag ctgcatgatc tgctgacgtt cgccaagtcg cgtcggttga gcgaaccgat
     2101 ccagcaaaag ctcggcgagc tgacccagct cgttgaaaat gcctactatt caccgcgcat
     2161 ctgtacgccg gaaatgctgg tcagtgccgc acagctcgtc gacgcgatct tggcaagtga
     2221 aaccgctgcg agcaaaagcc cagcccccga tctgcgggca ccgagcgcgc cacgaccgct
     2281 tatcaaccag ccgcgagcgt aagcgaagga gtccccgtgc gagttgctct cgtatctgac
     2341 atccatgcca atgaagtcgc gctgcgagcc gtttttcgcc atatcgacaa tctaggcgca
     2401 gatatcacga tatgtttggg ggatgtggcc acactcggac cgcacccgcg tgaagtattg
     2461 aacatattac aggagcgaaa atgtccgtgc atcatgggca atcacgacga gtttctcctc
     2521 gatcgccagc tcattgagcg ctacaccgaa gcaccgatca tcaaacaagc ggtcgattgg
     2581 tgccgcgatg aattatcaac acaagattta ga
//
LOCUS       DAORGN010000343         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_26388_length_2598_cov_4.504915, whole genome shotgun sequence.
ACCESSION   DAORGN010000343 DAORGN010000000
VERSION     DAORGN010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26388_length_2598_cov_4.504915"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKL17_21415"
     CDS             complement(<1..247)
                     /locus_tag="PKL17_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNK47357.1"
                     /translation="MADALGMIETKGFVGMVEAADAMVKAARVELVGYEKIGGGYVTA
                     VVRGDVAAVKAATEAGQRAAERVGELVSVHVIPRPHQN"
     gene            complement(317..958)
                     /locus_tag="PKL17_21420"
     CDS             complement(317..958)
                     /locus_tag="PKL17_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47358.1"
                     /translation="MATTTLRTYTFIDALQPQLASFMGKTAKGFLPVPGQASLFVEIA
                     PGIAINRVTDVALKATKVAPALQVVERAYGLLEVHDFDQGEVRQAGSSILNHLGISEQ
                     DRYKPRVLTNQIIRAIEPYQAQIINRNSQGMMILPGQSLFILETEPAGYVAFAANEAE
                     KAARIFLVSVTPYGAFGRLYLSGPEAEVDAAAAAATAALESIDGKQFDKFVDK"
     gene            complement(1035..1925)
                     /locus_tag="PKL17_21425"
     CDS             complement(1035..1925)
                     /locus_tag="PKL17_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47359.1"
                     /translation="MTADNVHLVFASPRVLPKAAKLSGRVAVLDVAFVADELGTSFSG
                     TTLKLIQDLGPRLAVWVDHHDHDRHPDFAADPRFVLAKKSEHGACPEMVSPELVARIG
                     PVDTILCHLDLDGLYAAAKWLRGGLEPYPGADADARAVDTRIGTPSPIADRIDRALRA
                     HFRDDDLKLAIVRYVASGAHDIELRQRIDRAAADFDDMEKETHRLAGLYQPRGRAVFV
                     DAQVHARTAFDKTELLLAGQALAQVAVVRHAGYVTLAAAFDSGVDFLKMFGISGGMPT
                     RVTLPEKRLGEVLARLCAIE"
     gene            complement(1922..>2598)
                     /locus_tag="PKL17_21430"
     CDS             complement(1922..>2598)
                     /locus_tag="PKL17_21430"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNK47360.1"
                     /translation="EVGLLPGVGPGLRFARHPLAYLVEAADDIAYCILDLEDGLRLQL
                     VPRQKGLLALRAVAEKAPHFAPPEDDETNDVVEYLRARAIKQLLIETQQAFMDCEASI
                     LAGTFQKGLLDCVPSAQLLRSIRGLSETYCYSSRAVLEIELAGYRLLGELLAELVPAA
                     VKTPSDRCQHEHKLLRLLPRIPDETTAYERVLRVTDFISGMTDSYAVSLYRKLRGISL
                     PGSVRW"
BASE COUNT          520 a          828 c          784 g          466 t
ORIGIN      
        1 tgttctggtg cggacgcgga atgacgtgca ccgagaccag ctcacccacg cgctccgcag
       61 cgcgctgacc ggcttcggtc gcggccttga cggcagcaac gtcgccacgg acgaccgcgg
      121 tcacgtaacc accgccgatc ttctcgtagc caaccagctc aacccgagcc gccttgacca
      181 tcgcatcggc ggcctcgacc atgccgacaa aacctttggt ttcgatcatt ccaagcgcat
      241 cagccatttt cgtaccctct tgttcgtgct cgtctccaag ggccggtctt tggtcccggc
      301 ccctgtgggc gattggttac ttgtctacaa acttgtcaaa ctgcttgccg tcgatcgatt
      361 cgagcgcggc ggtcgcggcg gctgccgctg catccacttc ggcttcgggg ccggacagat
      421 acagtcgacc aaacgcgcca tacggcgtca ccgacacaag gaaaatccgc gcggcttttt
      481 cggcttcgtt ggccgcaaag gccacgtagc ccgccggctc ggtttccaaa atgaacagcg
      541 actgtcccgg caggatcatc atgccctgcg agttgcggtt gatgatctgc gcctggtacg
      601 gctcgatcgc gcggatgatc tggttggtca gcacgcgcgg cttgtagcga tcctgctcgg
      661 aaatcccgag gtggttcaaa atcgatgagc cagcctgccg gacctcacct tgatcgaagt
      721 cgtggacttc gagaagaccg taggcgcgct caaccacttg gagcgccgga gccaccttgg
      781 tcgccttcag cgcaacgtcg gtgacgcggt tgatggcgat accaggggca atctcgacga
      841 acagcgaggc ctgaccgggc accggcagga agcctttcgc cgtcttgccc atgaagctcg
      901 caagctgcgg ctgcaaggca tcgatgaagg tataggttcg gagcgtcgtc gtcgccatgt
      961 cgatggggct ttcgcaaaaa gtggccggac gttaacaaag cgaaatcagt gggtcaaccg
     1021 cagaaatcca gcggtcattc gatcgcgcaa agtctggcca gcacttcgcc aagccgcttt
     1081 tccggcagcg tcacccgcgt cggcattccc cccgaaatcc caaacatttt gagaaaatcg
     1141 acgcccgaat cgaacgcggc ggccagcgtc acatagccgg catggcgaac caccgcaacc
     1201 tgggccagcg cttggcccgc cagcaaaagc tcggtcttgt cgaacgcggt ccgggcgtga
     1261 acctgcgcat cgacgaagac cgccctgccc cgcggctggt agagcccggc caagcgatgg
     1321 gtttcttttt ccatgtcgtc gaagtccgcg gcagcccgat cgatgcgctg ccgcagttcg
     1381 atgtcgtggg ccccggacgc cacgtagcgc acgatcgcca gcttcagatc gtcgtcgcga
     1441 aaatgagcgc gcagggcccg gtcgatccga tcggcgattg gggacggcgt gccgatgcgc
     1501 gtatccactg cgcgggcgtc agcgtcggca ccgggatagg gttcaagtcc ccctcgcagc
     1561 cacttggcag cggcgtacag accatccagg tccagatggc acaagatggt gtcgacgggc
     1621 ccgatgcgag cgaccagttc cggactgacc atttctggac acgcgccgtg ctcggatttt
     1681 ttggccaaca caaagcgcgg gtcggcggca aagtcaggat ggcggtcgtg atcgtggtga
     1741 tcgacccaga cagccaagcg cgggccaaga tcctgaatca gtttcagggt ggtgccggaa
     1801 aacgacgtgc ccagctcgtc ggcgacaaat gcaacatcaa gcaccgcgac ccggcccgaa
     1861 agcttggcgg cctttggcag cacccgcggc gaggcaaaga ccaaatggac gttgtctgcc
     1921 gtcaccagcg cacgctcccc ggaagcgaaa tgccgcgcag cttgcggtac agcgacaccg
     1981 catacgaatc ggtcatcccg gaaataaagt ccgtcacccg cagcacccgc tcgtaagcgg
     2041 tcgtttcgtc gggaatgcgc ggcaaaagtc gcaacagctt gtgctcatgt tgacagcgat
     2101 cggacggagt tttgaccgct gccggcacaa gctcggccaa aagctcaccg agcagccgat
     2161 agccggccag ctcgatttcc agcaccgctc gactgctgta acagtaggtt tcactcaggc
     2221 cgcgaatgct gcgcagaagc tgggccgacg gcacgcagtc gagcagcccc ttttggaatg
     2281 tgccagcgag gatgctcgct tcacagtcca taaacgcctg ctgggtttcg ataagaagct
     2341 gcttgatggc acgggcccgc aggtattcga cgacatcgtt ggtttcatcg tcttcgggcg
     2401 gtgcaaagtg cggcgctttt tccgccacgg cccgcagcgc caaaagaccc ttttgccgag
     2461 gcacaagttg cagccgcaaa ccgtcttcca agtcgagaat gcagtacgcg atgtcatcgg
     2521 cggcttcgac aagataggcc agcggatggc gggcgaaccg cagccccgga cctacgccgg
     2581 gcagcagacc gacttctt
//
LOCUS       DAORGN010000344         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_27369_length_2549_cov_4.813152, whole genome shotgun sequence.
ACCESSION   DAORGN010000344 DAORGN010000000
VERSION     DAORGN010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27369_length_2549_cov_4.813152"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="hemH"
                     /locus_tag="PKL17_21435"
     CDS             complement(<1..693)
                     /gene="hemH"
                     /locus_tag="PKL17_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNK47361.1"
                     /translation="MPTQGLLLLNLGTPDAPTPDAVRRYLREFLSDPRVIDIHPIARF
                     LLLHLVILPLRPRRSAEAYQKIWTPSGSPLLVHGRALAQAVQARLSDRWKVRLAMRYG
                     NPSLQTALHDLTDCERIVVLPLYPQYSSASTGSSLEELYKLASAKQVTPNLSVVPPFF
                     RDPGYLACFVARSRPIIEAERPDHVVFSYHGLPERQVKKCDPSKRHCLASNDCCAQLS
                     SVNQNCYRAQGYD"
     gene            complement(745..1686)
                     /locus_tag="PKL17_21440"
     CDS             complement(745..1686)
                     /locus_tag="PKL17_21440"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNK47362.1"
                     /translation="MTSSEKSSSAPHLLVAIVKALRPKQWTKNVLLYAGFLFTLNKKW
                     FLFQPSMWQALQKATLAMLLFTALASGIYLLNDVRDLPHDRLHPTKRKRPIAAGHVPP
                     WLALVLTLLLEVGGLWGAFALSPSFGWVGLAYVLIQLAYTYSLKHMVIIDVFVLASGF
                     VLRAVSGVLVLSADISPWLYIVTFLGALFLGLCKRRHELSLLAEDAGRHRQSLSHYST
                     ELLDQMIGIVTASTIMAYSLYTFSAQGLPPNHLMMLTIPYVLYGMLRYLYLVHMHKLG
                     GSPEEILLRDRPILAVLLLWAGTAAAILAYGRTLPAS"
     gene            1731..2300
                     /locus_tag="PKL17_21445"
     CDS             1731..2300
                     /locus_tag="PKL17_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNK47363.1"
                     /translation="MTRVAEFSIEEMFVRRWSPRAMSGQSVTEAELLRLFEAARWAPS
                     GGNSQPWRFAYAIAGEPEFARFFDLLADGNKIWCHRAGALIVVLSQAVLDNGKPYPPH
                     AFDAGAAWMSLALQGSAMGLVVHAMGGFDAERARTVLEVPDAQAVQVMIAVGHPGKVE
                     ELPEKLQPREQPSLRRPVRELIAKGRAPR"
BASE COUNT          531 a          734 c          828 g          456 t
ORIGIN      
        1 gtcatagccc tgggcgcgat agcagttttg attgaccgac gaaagctgcg cgcagcagtc
       61 gttgctggcg aggcagtgtc gcttgctcgg gtcgcacttt ttgacctgcc gttcggggag
      121 tccgtgatac gaaaacacga cgtgatcggg gcgctcggct tcgatgatcg ggcggctgcg
      181 ggcgacaaag caggcaaggt agccgggatc gcgaaaaaac ggcggcacca ccgacagatt
      241 cggcgtcacc tgcttcgcag aagcgagctt gtacagctct tccagcgacg atccggtcga
      301 cgccgatgaa tattgcggat acagcggcag cacgacaatg cgctcgcagt cggtcagatc
      361 atgcagcgcg gtttgcagcg acggatttcc gtaacgcatg gccagccgca ctttccatct
      421 gtccgaaagc cgcgcctgta ccgcctgtgc cagcgcgcga ccgtgaacca aaagcggcga
      481 gcccgaaggg gtccagattt tctgatacgc ctcggcactg cgacgcggac gcagcggcaa
      541 gatcacaagg tgtagcaaaa gaaagcgcgc gatcggatgg atgtcgatga cgcgcgggtc
      601 ggacagaaac tcgcgcagat aacgcctgac agcatccggt gtcggcgcat ccggcgtccc
      661 caagttcagc agcaaaagtc cttgtgtcgg catggtggcg cagaagagcc caggttggct
      721 gggaaatagg atgtcgccga tcgcctaact tgccggcaag gttcgtccat acgccaaaat
      781 cgccgctgcg gtgcccgccc acaacagcag caccgccaaa atcggtcgat cgcggagcag
      841 gatttcttcc gggctgccgc cgagcttgtg catgtgcacc aaatacaagt agcgcagcat
      901 gccgtagagc acatacggga tcgtcagcat catcaagtgg ttgggcggca ggccttgcgc
      961 ggaaaacgtg tagagcgaat aggccatgat ggtgctcgcc gtcacgatgc cgatcatctg
     1021 atcgagcagc tccgtcgaat agtgcgacaa gctctggcgg tggcgaccgg cgtcttccgc
     1081 gagcagcgac agctcgtgcc gacgcttgca cagccccaaa aacagcgcgc ccaaaaacgt
     1141 cacgatataa agccacggtg agatgtcggc tgacaggacc agcacgccgc tgactgctcg
     1201 cagcacaaac cccgacgcca gcacaaacac gtcgatgatc accatgtgct tgagcgagta
     1261 ggtgtacgcg agctgaatca gcacataggc cagaccgacc caaccaaagc tgggactgag
     1321 cgcaaaggct ccccacaggc cgcccacttc gagcagcagc gtgaggacga gcgcgagcca
     1381 cggcggcaca tgaccggcgg caatgggccg tttccgcttg gtcggatgca gcctatcgtg
     1441 cggcagatcg cgcacatcgt tgagcaagta gatccccgac gccagcgcgg taaacagcag
     1501 catggccagc gtcgcttttt gcagcgcttg ccacatgctc ggctgaaaca ggaaccactt
     1561 tttgtttagc gtaaaaagaa atcccgcgta gagcaggacg ttcttggtcc actgtttggg
     1621 gcgcagcgcc ttcacgattg cgacgagaag atgcggcgca gaagacgact tttcgctact
     1681 ggtcacggcg cggcatcata aacgacattg tgaacggcaa ggaaaagacg atgacccgag
     1741 ttgctgagtt ttcgatcgaa gagatgtttg ttcgccgctg gtcgccgcgc gcgatgagcg
     1801 gccagtctgt caccgaggct gaacttctgc gcctgtttga agcggcgcgt tgggctccgt
     1861 cgggcggtaa cagtcagccg tggcggttcg cgtatgcgat cgccggggaa cccgagttcg
     1921 cgcggttttt tgacctgctc gctgatggaa acaagatttg gtgccatcgg gcgggggcgc
     1981 tgatcgtcgt tttgtcgcag gcggtgctcg acaacggcaa gccgtatccg ccgcatgcgt
     2041 tcgatgctgg cgcggcgtgg atgagccttg cgcttcaggg gtcagcgatg gggcttgtgg
     2101 tccacgcgat gggcggtttc gatgccgagc gggcgcgcac ggtgctggaa gtgcccgatg
     2161 cgcaagccgt gcaggtgatg atcgccgtcg gacatccggg caaagtggaa gaactgccgg
     2221 aaaaactgca accccgcgag cagccgagcc tccgtcgacc cgtgcgcgag cttatcgcca
     2281 aaggccgggc cccgcgctag cccgtgatcc ccacgcggct gctggttttg cacaatcccc
     2341 gacggtgaaa aagccgtgat acgatagggg aatgggggat tttgcacgct gtttgggtta
     2401 cttggctgtg gtcggtctgc tgtcggcgac gggctgcgcg ggatcgaacg cggaaaaacg
     2461 gggcggtggc gggtccgcca agctgccgcc gcaggtgctc gatacatccg acgtaaatga
     2521 atcggttgcg gcgctttctg gttcatcgg
//
LOCUS       DAORGN010000345         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_27451_length_2545_cov_6.620884, whole genome shotgun sequence.
ACCESSION   DAORGN010000345 DAORGN010000000
VERSION     DAORGN010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27451_length_2545_cov_6.620884"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKL17_21450"
     CDS             <1..255
                     /locus_tag="PKL17_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNK47364.1"
                     /translation="HDQGMKFGLQASMAKLYAAQAAMRACNTAVQIHGGYGYTREFPV
                     EKAMRDAKLCEIGEGTNEIQRIVISRELLSRGELFVPGVV"
     gene            258..1424
                     /gene="lepB"
                     /locus_tag="PKL17_21455"
     CDS             258..1424
                     /gene="lepB"
                     /locus_tag="PKL17_21455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNK47365.1"
                     /translation="MANAESSVGAFFASVVSAFVTAWQDPERLFDADDLAATRKRAIR
                     FALTMFVLSYGIAAAGVCAIGFAVGQPGVLGAVALLLLVSGPLLSLLLLGLESLFVRI
                     FGPQPLPGPRAWDLAVSGVGFAQATMPVLIVPVIGMPLGLLLGARIIVSGMKKLGAAS
                     GERAWLSALVALGLPALLSLSMRTMVIETFRLPSASMHPTLLAGDLVLATKFDYGINL
                     PGKPRALAQSLPQLGDMVVFSRSDDEPQQFVKRVVGLPGDVIELRGQSLWRNGTPVET
                     QPSSEPCEFYDIDERQPPPGTLMKCRGQTEILAGVRYNVAWSLVHDTQAQPQTFTVPA
                     DQVFVLGDNRDNSHDSRHFGSIPVARVTAKVRRVLWSFSPTRQPRTERTFFPVR"
     gene            complement(1421..1978)
                     /locus_tag="PKL17_21460"
     CDS             complement(1421..1978)
                     /locus_tag="PKL17_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47366.1"
                     /translation="MSIVYRSVHHAGRPSRALATLSLLFSVGCVSSERYILDRTAAEQ
                     LAPNVVPPDERMSPQGLDYVAAVREKDRRPVFVKTSALRKDTLTPLSPDSFRIEARGY
                     NRLVTAGSILTWVGTAVSLAGTVIVAVAKREDNTPLFYAGGITALAAEPMMWTGTGLW
                     IYGALRLPFEKNPQPTTPTAGQPGL"
     gene            2261..>2545
                     /locus_tag="PKL17_21465"
     CDS             2261..>2545
                     /locus_tag="PKL17_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47367.1"
                     /translation="MSGNRKKRGTARWKSLTNERLLGSAVALSASVLSGCSTECLDNN
                     AFYEQKVWTQVVSKNCLKCHAPEGVAANKNAKFIQLSQSFPGFLDINLQNI"
BASE COUNT          480 a          730 c          803 g          532 t
ORIGIN      
        1 cacgatcaag ggatgaagtt tggtctgcaa gcgtcgatgg cgaagctgta tgcggcgcaa
       61 gcggcgatgc gggcttgcaa caccgcggtg cagatccatg gtggctacgg ttacacccgc
      121 gaattccccg tcgaaaaagc gatgcgcgat gccaagctgt gcgaaatcgg cgaaggcaca
      181 aacgaaatcc agcgcatcgt catcagccgt gagcttctca gccgcggtga gctgtttgtt
      241 cctggcgtcg tgtagacatg gcaaacgcag aatcttccgt cggggcgttc ttcgcctcgg
      301 tggtatcggc atttgtgacg gcgtggcaag atcccgagcg gctgtttgac gcagacgacc
      361 ttgctgcgac gcggaagcgg gccattcgtt ttgccctgac gatgtttgtg ctgtcttacg
      421 gcattgccgc cgcgggcgtc tgcgccatcg gttttgctgt cggacagcct ggcgtcttgg
      481 gcgcggtggc gcttttgctt ttggtgtcgg gaccgctgct ttccctgctg cttttggggc
      541 tggaatcgct gtttgtgcgc atctttgggc cgcagcctct gccgggtccg cgcgcgtggg
      601 acttggcggt gtcgggcgtt ggctttgcgc aagccacgat gccggtgctg atcgtgccgg
      661 tgattggcat gccgctaggg cttctgctcg gcgcgcgcat catcgtcagc gggatgaaaa
      721 agctcggcgc cgcaagtggc gagcgggcgt ggctgtcggc actggttgca ctggggctgc
      781 cggcgcttct gtctttgtcg atgcggacga tggtcatcga gacattccgt ttgccatcgg
      841 ccagcatgca tccgacgctg ctcgccgggg atttggtgct ggcgaccaag ttcgactacg
      901 ggatcaacct gccgggcaaa ccgcgcgccc tggcgcagag cctgccgcag cttggtgaca
      961 tggtggtgtt ttcacgcagc gacgatgaac cgcagcagtt cgtcaagcgc gtggttggtc
     1021 tgcccggcga tgtcatcgag ctgcgcggcc agtcgctgtg gcgaaacgga acgccagtcg
     1081 agacgcagcc atcgagcgag ccctgtgagt tttacgacat cgacgaacga cagccgccac
     1141 cgggcacgct catgaagtgt cggggacaga ccgagatcct ggcgggcgtc cgctacaacg
     1201 tggcgtggag tcttgtgcac gacacccaag cgcagccgca gacgttcacg gtgcccgcag
     1261 atcaagtgtt tgtgctgggt gacaaccgcg acaacagcca tgacagccgc cactttggat
     1321 cgattccggt tgcgcgggtc acggccaaag tccgtcgcgt gctgtggtcg ttttctccga
     1381 cgcggcagcc ccggacagaa cgcaccttct ttcccgtccg ctagagaccc ggctgccctg
     1441 ccgtgggtgt ggtcggctgc ggattttttt caaacggcaa gcgcagcgcg ccataaatcc
     1501 acagtccggt tcccgtccac atcatcggct ctgcagcaag tgcagtgatt ccgccggcat
     1561 aaaacagcgg cgtgttgtct tcacgctttg ctaccgccac gatcaccgtt ccggccagcg
     1621 acaccgcggt tcctacccac gtcaaaatcg atccagcggt gacaagtcga ttgtatccgc
     1681 gcgcttcgat gcggaacgaa tccggcgaaa gcggagtcag ggtgtctttg cgcagcgccg
     1741 atgtcttcac aaacactggg cgacgatctt tttcgcgcac tgccgcgaca tagtccaaac
     1801 cttgcggaga cattcgctcg tccggcggaa cgacgtttgg cgcaagctgt tccgccgctg
     1861 tgcgatcgag tatgtagcgc tccgaagata cgcacccgac ggaaaagagc agggacaagg
     1921 tggcgagtgc gcggcttggc cggcctgcgt gatgaaccga tcggtacacg atggacataa
     1981 atcagtaggc tccttgtcta ttttggatgt tgtgcactct gccgcaaacg tcagcgcgga
     2041 ttctcaaacg acatcgcagg gtcagaagga atctgacgta aggtttgctg cgctacgacg
     2101 acatccgtaa tctgatcgat gctgccatga gctgtcatgg ctggggctca ctacatgggg
     2161 gtgagctcgt caggataccc atgtcacaca tgccgtcggg gggcgacgca atgcgattgt
     2221 acgtgccgca accacggcgg cagcagaagg agccagttgc atgagcggga acaggaaaaa
     2281 gcgaggcacc gcgcggtgga aaagcctcac aaatgagcgc ctattgggaa gcgcggtcgc
     2341 actgtctgcc tccgtcttaa gtggttgttc tactgagtgc cttgataaca atgccttcta
     2401 cgagcagaaa gtttggacgc aagtcgtctc gaaaaactgt ctgaagtgtc acgctccgga
     2461 aggcgtggcc gcgaacaaga acgcaaagtt catccagctt tcgcagtcat tccctggctt
     2521 ccttgacatc aacctgcaga acatt
//
LOCUS       DAORGN010000346         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_28668_length_2486_cov_5.023447, whole genome shotgun sequence.
ACCESSION   DAORGN010000346 DAORGN010000000
VERSION     DAORGN010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28668_length_2486_cov_5.023447"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PKL17_21470"
     CDS             <1..1373
                     /locus_tag="PKL17_21470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47368.1"
                     /translation="RQIASALAATHAKGIIHRDLKPDNVMLVPDSEVAGGERVKILDF
                     GIAKVITDYSGPGAEEFKTNTGLVLGTATYMAPEQCKGAGDVSEKADVYSLAVVMYRM
                     CCGKVPFRAEGPGEVMAMHIFSQPQPLRDIEPRIPEPLAAIIHRMMSKEPHERPTMAQ
                     VAGELEKMAQTATAAAVVPAAKSTGSSGAVPIPGSASAPTVDAPPSAGSSGASAASQP
                     STIGTAVGQSGSAPAVTVGSRSRLGLWFAMVLVLGGTGVSIPYALKMYPQLFAAKAKP
                     VYWNISCSPVAQVVRKSDGQAMGPTPWTHSVLQGTGRQLFVLRAEGYRDQEVWFDESS
                     SATQTVVLQAEPKPSEVVVDGGTAGSAPVIVIQDGGLPGSTGGPDGGTKPGAAIARQP
                     IGTPSAQAVEKVGNGVATVPGVKTGRPAAMGNPGNRTNGNSKLPRLGSDAELLKDDDI
                     AILKDD"
     gene            complement(1391..>2486)
                     /locus_tag="PKL17_21475"
     CDS             complement(1391..>2486)
                     /locus_tag="PKL17_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47369.1"
                     /translation="PAPMLSPVAPPSLCGILRSMFVLCGLLVLGLPSVLSGTVAADAG
                     VEYDSNPARVPSDGSLDVLPQGAPLFRGLISGSLAYAGPTQRLRLQATTTGKVFLTPE
                     QQQQNVLVAQFGYEHGAKIGRSLLGGFVDYYDAFQAAAPTYLSRDFRSLSVGGRYQGA
                     RPFENGHRIDGGLQLNGQLFHYKPDSLQSFVGPSLLARIGGRIHKGDPELGHDFDLSL
                     WGRGDLRVYGPVRADVFLSTGASVQWQGPLLIQVGYSAQLNLSTTELESYQRHLPFVK
                     IAFPLPGELFVTAKAQLNLVKATQGLTVPLQNIDEDNRSLALLDIERPLPKGFAVSAR
                     YSGFFSLPSDGTAPYQRHTGLLNVSYRFTR"
BASE COUNT          495 a          721 c          843 g          427 t
ORIGIN      
        1 gccggcagat cgcctccgcc ctcgccgcca cccacgccaa gggaatcatc caccgagacc
       61 tgaagccgga caatgtgatg ttggtgccgg attcggaggt ggcgggcggg gagcgggtca
      121 agatcctcga ctttggcatc gcgaaggtca tcaccgatta cagcggtccg ggggccgagg
      181 agttcaagac gaacactggt ttggtgctcg ggacggcgac gtacatggcg cccgagcagt
      241 gcaaaggtgc cggcgatgta tcggaaaagg ccgatgtcta ttcgctcgcg gtcgtgatgt
      301 atcggatgtg ctgcggcaag gttccgttcc gcgccgaggg tccgggcgaa gtgatggcga
      361 tgcacatctt ttcgcaaccg cagccgctgc gcgacatcga gccgcggatt cccgagccac
      421 tggcggcgat catccaccgg atgatgtcca aagaacccca cgagcgtccg acgatggcgc
      481 aggtggcggg tgaactggaa aagatggcgc agaccgcgac ggcagccgcc gtggtgccag
      541 ccgccaagtc gacgggctca tcgggggctg ttcccattcc gggttctgcc agtgcgccga
      601 ccgtcgatgc accaccgtct gccggttcca gcggggccag cgcggccagt cagccgtcga
      661 cgatcggcac ggcagtggga cagagcggca gtgctccggc ggtgactgtg ggctcgcgtt
      721 cgcggcttgg gctgtggttt gcgatggtgc tggtgctcgg tggtacgggc gtcagcatcc
      781 cttacgcact gaagatgtac ccgcagcttt tcgcggcgaa agccaagccg gtgtactgga
      841 acatctcttg ttcgccggtg gcgcaggtgg ttcgcaagtc tgatgggcaa gcgatggggc
      901 cgacgccgtg gacgcattcg gtgctccagg ggacggggcg acagctcttt gtgctgcgcg
      961 ccgaggggta tcgtgaccaa gaggtctggt ttgacgaatc ctcgtcggcg acccagacgg
     1021 ttgtgctgca agcggaaccc aagccgagcg aagtggtcgt cgatggggga accgcaggca
     1081 gtgcgccggt gatcgtgatc caagatggcg ggctgccggg ctcgacgggt gggcctgatg
     1141 gcggaaccaa gcctggggcg gcgattgcgc gtcagccgat cggcactccg tctgctcaag
     1201 cggtggaaaa agtcggaaac ggcgtcgcga ccgtgccagg cgtcaaaacc gggcgaccgg
     1261 cagcgatggg aaatccgggc aatcgcacaa acggcaacag caagctgccg cggcttggtt
     1321 cggacgctga gctgctcaaa gacgacgaca ttgcgattct gaaagacgac tgatcgaatg
     1381 tgcgacgtcg ctaccgagtg aagcgatagc ttacgttgag cagtccggtg tgacgctgat
     1441 agggggccgt accgtcgctc gggaggctga aaaagccact gtagcgggcg ctcaccgcaa
     1501 agcctttggg cagcgggcgc tcgatgtcca agagagccaa gctgcggttg tcttcgtcga
     1561 tgttttgcag tggcaccgtc agtccttgtg tcgctttgac cagattcagc tgcgcttttg
     1621 ccgtcacgaa cagctcgccc ggcagcggaa acgcgatttt cacaaacggc aggtggcgct
     1681 ggtagctttc cagctctgtc gtcgacaagt tgagttgcgc gctgtacccg acttgaatca
     1741 gcagcggacc ttgccactgg acgctcgcgc cggtggacaa aaatacgtcg gcacgcaccg
     1801 gaccatagac acgaagatcg ccgcgacccc acagcgacag atcgaagtcg tggccaagct
     1861 cgggatcgcc tttgtgaatg cgaccgccga ttctggcgag cagcgacggt ccgacgaagc
     1921 tctgaaggct gtcgggcttg tagtgaaaaa gctgtccgtt tagctgcaat cccccgtcga
     1981 tgcggtggcc attttcgaat ggacgcgcgc cttgataacg accaccgacc gacagggatc
     2041 gaaaatcacg cgacagatac gtcggtgcgg ctgcttgaaa cgcatcgtag tagtcgacaa
     2101 aaccgcccaa cagcgatcgc ccgatcttcg cgccgtgctc gtaaccaaac tgcgcgacca
     2161 ggacgttttg ttgctgctgt tcgggcgtca aaaagacttt gccggtggtg gtggcttgca
     2221 accgcagccg ctgcgtcggg ccggcgtaag ccaaactgcc cgaaatcaaa ccgcgaaaaa
     2281 gcggcgcacc ttgaggcaag acatcgagcg atccgtcgga cgggacgcgg gcggggttgc
     2341 tgtcgtactc cacgccggca tcggcagcga cggtacccga cagcaccgac ggcaaaccga
     2401 ggacgagtag cccgcacagc acgaacatcg agcggagaat accgcaaagc gacggcggcg
     2461 cgactggtga tagcatcggg gcagga
//
LOCUS       DAORGN010000347         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_30215_length_2413_cov_7.111535, whole genome shotgun sequence.
ACCESSION   DAORGN010000347 DAORGN010000000
VERSION     DAORGN010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30215_length_2413_cov_7.111535"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKL17_21480"
     CDS             complement(<1..410)
                     /locus_tag="PKL17_21480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47370.1"
                     /translation="MSLDRIGPYQVVRKLGEGGMGAVFEAVQDPIGRRVAIKILHPKY
                     AQDPGICARFFNEARAVNLIDHPGVVQISDYGQLPDGTAYLVMEYLKGETLSQRQKSK
                     GMPPLPEIMRLARQIAAALSAAHEKNVYHRDLKP"
     gene            579..1226
                     /locus_tag="PKL17_21485"
     CDS             579..1226
                     /locus_tag="PKL17_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47371.1"
                     /translation="MSLKRGPLARRLTLLGVLAMGLFFAVHLHAGPELRSREWAIDGF
                     RPPPRWDLTPRDRPSYPQLIAWASRGDGSERAVISLVGKRVAAHTTIRDLLPEAQAIK
                     SAPQIENVRIQVLSTLGWTTGWTGNVRIQVDANLPAQGKDRARVVRQYLFLNGPVAYT
                     MTLVAPLEQATARLRDLDDTAANLVPIDPEGVVSTATTNHPDGGLRPDLANPTTK"
     gene            1434..>2413
                     /locus_tag="PKL17_21490"
     CDS             1434..>2413
                     /locus_tag="PKL17_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47372.1"
                     /translation="MSTSRTLFSLALVALVAGSTGCDTLRARARAQKGVEKYRDGDIA
                     EAAKLFREAAEIDPTVPPIIINRGFTHLALYQLNPRSKDGQEAANIAVQSFRQYMDMP
                     KLKPEQRQRARDYLLQTFVDAKKYDEAVDFFKPQVERDNPDIEALTILGNIAKSIGKL
                     DACRMWLERRVKANPKDTDGYVSLAIMTWDELCNDSQCRTAMELRKNHLQMPFADRIA
                     KANQALDLIEFARQLSPKAPTPVIYGNLLLRERSYAQPNDELKRQDLDDAGKLLQTAL
                     AMQKAATEAKAAAEAAKPGEPAKPGEPAKPAEAAKPGEPAKPAEAAKPAEA"
BASE COUNT          480 a          724 c          768 g          441 t
ORIGIN      
        1 tctggcttca ggtcgcggtg atagacgttc ttttcgtgcg ctgcgctcag tgccgctgcg
       61 atctgccgag cgagccgcat aatctccggc agcggcggca tgcctttgga cttttgccgc
      121 tggctcagcg tttcgccctt gaggtactcc atgacgagat acgcggtccc gtcgggaagc
      181 tgcccatagt cggagatttg cacgacccca ggatggtcga tgaggttgac ggccctggct
      241 tcgttgaaaa agcgtgcgca gatgcccgga tcttgcgcgt atttcgggtg gaggatcttg
      301 atcgcaaccc gacgaccgat ggggtcttgg acggcctcaa agaccgctcc catgccacct
      361 tcgccgagct tgcggacgac ctgatatgga ccaatacgat cgagagacac tttgcggctc
      421 ttacacagca ctataccgcc ttggtgcttc tttgtcgtca ccttgtgttg tttgaaatac
      481 gtaagaatca aaatgagaag tcggggactg catcccagca ggtaggtggt tgtcgggcgc
      541 tgtcccttcg ccaagatgct gataggctag ggcccatcat gagtctgaaa cgaggaccgc
      601 tggcccggcg cctgaccctg cttggggtgt tggccatggg gctgtttttt gcggttcatc
      661 ttcacgctgg gcccgagctg cgcagtcgag agtgggcgat agacgggttt cggcctccgc
      721 cgcgctggga tttgacgcca cgcgaccggc caagctatcc gcagcttatc gcttgggcca
      781 gtcggggcga cggcagcgag cgggcggtga tcagcttggt cggcaagcgc gtcgcggctc
      841 acacgacgat tcgtgatctg ctacccgagg cgcaagcgat caagagcgct ccgcagatcg
      901 aaaacgtgcg catccaagtg ctgtcgacgc tgggctggac gacgggctgg accgggaatg
      961 tgcggattca ggtcgacgcc aacttgcccg cccaagggaa agatcgtgct cgcgtcgtcc
     1021 gtcagtacct gtttctgaac gggccggtcg cttatacgat gacgctggtg gcgccgctcg
     1081 aacaggcgac ggcgcggctg cgtgatctgg atgacacggc agcgaatctg gtgccgatcg
     1141 atccagaggg agtggtttcg acagcgacga ccaatcatcc cgatgggggc ctgcgacccg
     1201 acctcgcgaa tccgacgaca aagtgacggg cgggtctgcg ccgtggcgaa agaggttggg
     1261 ccgttagact ctcaacacgg cggctttgca gatggatcgg ctgtggccgg caaaaacccg
     1321 cggcagacga cgcgccgcgg gctgtggggc ggttgtgtaa ctcaggaaaa atacacggct
     1381 caacacgaaa cccttgcgat gggtcggccc agattggtag cgttccgcgc atcatgagca
     1441 cgtcccgcac cctcttttcg ctcgccttgg tcgcgcttgt ggctggttcc acgggctgcg
     1501 acacgctgcg tgctcgtgcg cgcgcgcaaa aaggcgtgga aaagtaccgc gacggggaca
     1561 tagccgaggc ggccaagctg tttcgtgagg ccgccgagat cgatcccacc gtccctccga
     1621 tcatcatcaa tcgcggcttt acccacctcg cgctttatca gctcaatccc cgcagcaagg
     1681 atgggcagga agccgccaac atcgccgtcc agtcgttccg gcagtacatg gacatgccga
     1741 agctcaagcc tgagcagcgg cagcgcgccc gtgactacct gctgcagacg ttcgtcgatg
     1801 ccaaaaagta cgacgaggca gtcgacttct ttaagccgca ggtcgagcgc gacaatccgg
     1861 acatcgaagc actgacgatc ctgggcaaca tcgccaagag catcggcaag ctggatgcat
     1921 gtcggatgtg gctggagcgc cgcgtcaaag cgaatccgaa ggacaccgac ggatacgtct
     1981 cgctggcgat catgacctgg gacgagctgt gcaacgattc gcagtgccgg accgcgatgg
     2041 agctgcgcaa aaaccacctg cagatgccgt ttgcggatcg cattgccaag gcgaaccaag
     2101 cgctcgattt gatcgagttt gcccggcagc tttcgcccaa ggctccgacg ccggtgattt
     2161 acggcaactt gcttttgcgg gaacgctcct acgcgcaacc gaacgacgaa ctgaagcgtc
     2221 aggatctgga tgacgccggc aagcttctgc aaaccgcgct ggcgatgcag aaagcggcca
     2281 ccgaagcgaa ggccgctgcc gaagcggcca aacccggtga gcccgccaaa cccggtgagc
     2341 ccgccaagcc tgcggaagcg gccaagcccg gtgagcccgc caaaccagcg gaagcggcca
     2401 aaccagcgga agc
//
LOCUS       DAORGN010000348         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_30358_length_2407_cov_4.571429, whole genome shotgun sequence.
ACCESSION   DAORGN010000348 DAORGN010000000
VERSION     DAORGN010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30358_length_2407_cov_4.571429"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="murD"
                     /locus_tag="PKL17_21495"
     CDS             <1..1142
                     /gene="murD"
                     /locus_tag="PKL17_21495"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNK47373.1"
                     /translation="GTNGKSTVTSLCGAIAKATSRPSFCGGNLGTPLTDAVGTAAGGP
                     QGIVVIELSSYQLETCESLSCQAAAILNLTPDHLDRYPSMRQYGDAKARIFRNMAPWS
                     TKVTSADDPEVQALYARHLGSNDPCFLFTTQSTLPSRRVQGRAIQIGGFVDGGDLVLR
                     GAVGGLQAVLSGREPVEERYPLQEFQLVGRHNQANMLAAFLLMRASGLASYEQVRRAA
                     TTFSALPHRMQLVGESGGVAFFDDSKGTNVDAVVAGLAGFPRPLVLIAGGRDKGGSYE
                     PLRKTLCEQTTRGVVVIGEAAPLIEQSLAGCPFPILHADSMQSAVGQAMSLCQSGDAV
                     VLSPACSSFDMFRDYAHRAAVFCESVASLPGAKLSASHSRGGAHS"
     gene            1139..2350
                     /gene="ftsW"
                     /locus_tag="PKL17_21500"
     CDS             1139..2350
                     /gene="ftsW"
                     /locus_tag="PKL17_21500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNK47374.1"
                     /translation="MKNEEVTLPQPHRADPLLFVVVLLLVGLGLVMVYSSSAVLAQQK
                     MGDSRYFLVRDLVWVLLGLVGMALTMRIDYSLYRRLAYPILGTATLLLSAVLVVGARV
                     NGARRWFHVGPLSFQPAELAKVALIVFLAHILAKKADKVRHFLLGFLPPIVVCAMFAG
                     LLLKQPDLGTAMIVGGTTIVMLFVAGTRVSYLAIAGLGALPIIYNAIVGTPWRLRRIL
                     AFLDPWQFRDTFGYQMAASLIAVGSGGLTGQGLGDSRQKLLFLPEAHTDYILAIIGEE
                     LGFIGIAFVVGLYVILILRGCATAARARDSFGMYLAVGITAMFGLQAAFNIGVVLGAL
                     PTKGLTLPFLSFGGSTLVIDLGAIGVLLNISRREPAPIEKKSKNRSWRSLFHRRKNRR
                     RALPRTAEASA"
BASE COUNT          404 a          720 c          765 g          518 t
ORIGIN      
        1 ccggcacaaa cggcaaatcg accgtgacca gtctgtgcgg cgccattgcg aaagcgacgt
       61 cgcgcccgag cttttgcggt ggcaacctcg gtacaccgct caccgacgct gtgggcaccg
      121 ccgccggagg accgcaaggc atcgtcgtca tcgagctgtc atcgtatcaa ctggagacct
      181 gcgagagcct gagctgtcag gcggcggcga ttctaaactt gacgcccgat cacctggatc
      241 gctatccgtc gatgcggcag tacggcgacg ccaaagcgcg cattttccgc aacatggcgc
      301 cgtggtcgac caaagtgaca agcgccgacg atcccgaagt gcaggcactg tatgcgcgcc
      361 acctggggtc aaacgatccg tgctttctgt ttacaaccca gtcgacgctg ccgtcgcggc
      421 gtgttcaagg ccgtgcgatc cagatcggtg gatttgtcga cggcggtgat ctggtgctgc
      481 gtggcgcagt cggcgggctt caggccgtgc tgtccggacg agaacccgtc gaagaacgct
      541 atccgctcca agaatttcag ctcgtcggtc ggcacaacca ggccaacatg ctggcggcgt
      601 ttttgctgat gcgggcgtcg ggacttgcaa gctatgagca agtccgtcgg gccgcgacga
      661 cgttttccgc gctcccgcat cgcatgcagc tggtgggaga atccggcggg gtcgcttttt
      721 ttgacgacag caaaggaacc aatgtcgatg ccgtcgtcgc tggactcgcg ggttttccca
      781 ggccgctggt gctgattgcc ggcggacgcg acaagggtgg ttcgtatgaa ccgctgcgca
      841 agacgttgtg cgaacagacc acccgcggcg tcgtcgtgat aggcgaagca gccccgctga
      901 ttgagcagtc gctcgcgggc tgtccattcc cgattttgca cgcagactcg atgcagagcg
      961 ccgtcggtca agcgatgtcg ctgtgtcagt cgggggatgc ggtggtgctg tcgccggcct
     1021 gttcgtcgtt tgacatgttc cgtgactatg cacatcgcgc cgcggtgttc tgtgaatccg
     1081 tcgccagcct acccggtgca aagctttcgg ccagtcacag cagaggcgga gcccactcgt
     1141 gaaaaacgag gaagtaacgc tgccgcagcc gcatcgggcc gatccgcttt tgtttgtggt
     1201 cgtgctcctg ctcgtcgggc ttggcctggt gatggtgtat tcatcgtcgg cggtcctggc
     1261 gcagcaaaaa atgggagaca gtcgctattt cctcgtccgc gatctggtct gggtgctgct
     1321 cgggctcgtc ggaatggcgc tgacgatgcg gatcgactat tcgctctatc ggcggctcgc
     1381 gtatccgatc ctcggaacgg cgacgctgct gctttccgcg gtgctcgtcg tcggagcgcg
     1441 tgtcaatggt gcccgtcgct ggttccacgt cggaccgctg tcctttcaac ccgccgagct
     1501 ggccaaggtc gcgctgattg tctttttggc gcatatcctg gccaaaaaag ccgacaaggt
     1561 gcggcacttt ctcctcgggt ttttgccccc gatcgtcgtc tgcgcgatgt ttgccgggct
     1621 cttgctcaag cagcccgatc tgggcaccgc gatgatcgtc gggggcacca cgatagtcat
     1681 gctcttcgtc gcgggaaccc gcgtcagtta cctggccatt gcgggtcttg gcgccttgcc
     1741 gatcatctac aacgccatcg tcggaacacc gtggcgtctg cgacggattt tggcctttct
     1801 tgatccgtgg cagtttcgcg acacgttcgg ctatcaaatg gcggcgtcgc tgattgcggt
     1861 gggttccggt ggactcaccg gccaggggct tggcgacagt cgacaaaaac tgctgttttt
     1921 gcccgaagcc cacaccgact acatcttggc gatcatcggc gaggagctgg gttttatcgg
     1981 catcgccttc gtcgtcggac tgtacgtgat cctgattctg cgcggctgtg cgacggcagc
     2041 gcgggcccgc gattcgtttg ggatgtatct ggccgtggga attaccgcga tgtttggact
     2101 gcaagcggcg ttcaacatcg gcgtcgtcct cggagcgctg ccgaccaaag ggctgacact
     2161 gccgtttttg tcgtttggtg gctcgacgct ggtcatcgat ctgggtgcca tcggcgtgct
     2221 gctcaacatt tcgcgacgag aaccggcccc gattgaaaag aagagcaaaa atcgatcgtg
     2281 gcgcagcctg tttcatcgac gcaaaaaccg tcgccgcgca ctacctcgca cagcggaagc
     2341 aagcgcatga gtgaaaagcc ccgtcccctc tcggcagcgg gcgttcgcgt cgtgatcgca
     2401 ggtggcg
//
LOCUS       DAORGN010000349         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_31620_length_2356_cov_6.612342, whole genome shotgun sequence.
ACCESSION   DAORGN010000349 DAORGN010000000
VERSION     DAORGN010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31620_length_2356_cov_6.612342"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKL17_21505"
     CDS             <1..459
                     /locus_tag="PKL17_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47375.1"
                     /translation="NGETWVRIDMIKVMDTDSESAHDLKIQEARDSYRSRQRLKYPDG
                     KSLEDVGRAHVSGFVTYKVKGVTPGKRLLIVRRMDYVYGDYSLEFEVDGKKAGICECP
                     GTDRINRWRNWPHIIPAEFVVRAEPNVKQIAVTAGRDINMFRYWFYQPRQ"
     gene            477..1376
                     /locus_tag="PKL17_21510"
     CDS             477..1376
                     /locus_tag="PKL17_21510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNK47376.1"
                     /translation="MFLDQIIDLIMRPFRELWNVIARVRGVQSGALADVRRVQYLGDS
                     ARSEVAGMKNIVVAPGQIVPGWGSSQPAAPPPQMAQQQAEKKKMGWSFPWSKKTCPRC
                     QNKLQKSWDQCPYCGQNQNQPVTAGPPGAAPGAPGAAPGYGAPPGGPGYGAPPGAPGY
                     GAPGGAPGYGAPMPSPGGAAKTIAMDAAAIDAPLLATGDTGENVAWLVPLEGAMTGEL
                     LQIRTRAIIGTAEGCEIKLFDAAISGRHAEVVLSQGSRFRINDLGSRNGTYVNDKAIA
                     STELVDGDNIRLGRTTFRFKTKH"
     gene            1386..>2356
                     /locus_tag="PKL17_21515"
     CDS             1386..>2356
                     /locus_tag="PKL17_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNK47377.1"
                     /translation="MHCRSLFQTTSGNSVFRLLARFLPTVALLFTLLAPGLALAQNAK
                     LRLERFGFDKLPDLKVYITYVEEDGTVIAGRQATDFKLQVDSIDQGAGRAITTFDQAK
                     EPIFVVAVVQVGGAVDERVLSEVKGGLRRVNDAVGNTPGGRMGVLAYADKTNRILEFG
                     TTTEVDASIGKIALETEASESHMLDAVRTAIDLLKQQEKGRRKLIILVSDGIDVNNEK
                     KNFTEIGNRAQLGGIVIDTVGYNAFDASKLRNLQEMTKRCYGTDRVAKAPTEIGPKLD
                     GIVDEIQKQYLVTFPLTIAGDDQDHLFQVTHEAGGKPVYSNTIQEKLL"
BASE COUNT          504 a          727 c          722 g          403 t
ORIGIN      
        1 aacggcgaga cctgggtccg catcgacatg atcaaggtca tggacaccga ctcggagagc
       61 gcccacgacc tgaagatcca agaggcgcgc gactcgtacc gatcgcgtca gcggctcaag
      121 tatcccgacg gcaagtcgct cgaagacgtc ggtcgtgcgc acgtctcggg cttcgtcacg
      181 tacaaggtca aaggcgtcac gcccggcaag cgactgctca tcgtccgtcg catggactac
      241 gtctacggcg actactcgct ggagttcgaa gtcgacggca aaaaggccgg catctgcgag
      301 tgtccgggca ccgaccgcat caaccgctgg cgcaactggc cgcacatcat tccagcggag
      361 tttgtggtgc gcgccgagcc gaacgtcaag caaatcgccg tcacggctgg ccgcgacatc
      421 aacatgttcc gttactggtt ctaccagccg cgacaataat gtaagctgga cgggtcatgt
      481 ttctcgacca gataatcgac ctcatcatgc gaccgttccg cgaactgtgg aacgtgatcg
      541 cgcgggtgcg cggtgtccag agcggcgcgt tggccgacgt gcggcgagtg caataccttg
      601 gggatagcgc ccgctccgag gtcgccggca tgaagaacat cgtcgtcgct cccggccaga
      661 tcgttcctgg ctggggttcg tcgcagccgg cggcgccgcc gccccaaatg gcgcagcaac
      721 aggcggaaaa gaaaaagatg ggctggtcgt ttccttggag taaaaaaacg tgtcctaggt
      781 gtcagaacaa gctgcagaaa agctgggatc agtgccccta ctgcggccaa aatcagaacc
      841 agccggtcac cgccggaccg ccaggggctg caccgggggc gccaggggct gcgccgggct
      901 acggagcacc tccgggtgga ccgggatacg gggcccctcc gggcgcgcca ggctacggag
      961 cgccaggtgg agcaccgggt tacggcgccc cgatgccgtc gccaggcgga gcggccaaga
     1021 cgattgcgat ggatgcggcg gcgatcgatg caccgctgct cgcaacgggt gacaccggcg
     1081 aaaacgtcgc gtggctggtc ccccttgaag gcgcgatgac gggtgagctt ttgcaaatcc
     1141 ggacgcgcgc gatcattggc accgccgaag gctgtgagat caagctgttc gatgctgcca
     1201 tctcgggtcg ccacgcggaa gtggtgctgt cgcaaggcag ccgcttccgc atcaacgact
     1261 tgggatcacg caatggcacg tatgtcaacg acaaggccat tgcctccacc gagctggtcg
     1321 acggcgacaa cattcgcctg ggtcgtacca ccttccgctt caagaccaag cactaagggc
     1381 agaccatgca ctgtcggtca cttttccaaa cgacttcggg taattcggtc ttccggctgc
     1441 tggcccgttt ccttccgacg gtggcgctgc tttttacgct gcttgcaccg gggcttgcgc
     1501 tggcgcaaaa cgccaagctg cgtctcgaac gctttggttt tgacaagctg cccgacctca
     1561 aggtctacat cacctacgtc gaggaagacg gcaccgtcat cgctggtcgg caagcgacgg
     1621 actttaagct tcaggtcgac tcgattgacc agggcgccgg gcgcgcgatt acgacgtttg
     1681 atcaggccaa agagccgatc tttgtcgtcg cggtggttca agtcggcggc gccgtcgatg
     1741 agcgcgtgct ttccgaagtc aaaggcggtc tgcgccgtgt caacgatgcc gttggcaata
     1801 cgccgggcgg acggatgggc gtgctcgctt acgccgacaa aaccaaccgc atcctcgagt
     1861 ttggcaccac cactgaagtc gacgcgagca tcggcaagat cgcactggag accgaagcca
     1921 gtgaatcgca catgctcgac gcggtgcgca cggcaatcga cctgctcaag cagcaggaaa
     1981 aaggccgtcg caagctcatc atccttgtgt ccgacggcat cgacgtcaac aacgaaaaga
     2041 aaaacttcac cgaaatcggc aaccgcgccc agctcggcgg catcgtcatc gacaccgtcg
     2101 gctacaacgc atttgatgcg tcgaagctgc gcaacttgca ggaaatgacc aagcgctgct
     2161 acggcaccga tcgcgtcgcc aaagcaccga cggaaattgg tccgaagctc gatggcattg
     2221 tcgacgaaat ccaaaaacag tacctggtga cgtttccgct gaccatcgcg ggcgacgatc
     2281 aagaccacct attccaggtc acgcacgaag ccggtggcaa gccggtctac agcaacacga
     2341 ttcaggaaaa gctgct
//
LOCUS       DAORGN010000350         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_32927_length_2303_cov_4.896352, whole genome shotgun sequence.
ACCESSION   DAORGN010000350 DAORGN010000000
VERSION     DAORGN010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32927_length_2303_cov_4.896352"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..765)
                     /locus_tag="PKL17_21520"
     CDS             complement(175..765)
                     /locus_tag="PKL17_21520"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNK47378.1"
                     /translation="MGPLRTGQPEHIVATGAALGLLELQSIARGLTVADALVKRAAVQ
                     LHLNRPVSPGKHLVLCSGDVASVEEAMEAGRAQAGAALCDELLLTQVHDEVVDVLRRK
                     DKTPLGEDLSIGILETLSAASTLRGADAACKAAEVRLHDLRLCDGLGGKGFAILVGEQ
                     DMVQAALATVEQLLAPGLLLSVELIARPHPDFLQTL"
     gene            complement(790..>2303)
                     /locus_tag="PKL17_21525"
     CDS             complement(790..>2303)
                     /locus_tag="PKL17_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HNK47379.1"
                     /translation="DEKRIQEIVDKVLLRLSPDVQQTPAEAVMKAADKGTPNTGTSQR
                     PGAFVPPHLRGGGAGKRGDARVPRGTHGLFQDIDSATAAAQKAFEQYSSAPLSTREKM
                     VAAMRKVSLDNVKLLAEYAVAETGLGRVDDKIKKNTLVATKTPGPEILRPTAFSGDHG
                     LTVLDRAAYGVIGAITPTTNVTETILNNGIGMLAGGNAVVFNTHPYAWRTCAWFIHIL
                     NEAIVQAGGPGNLLCSVAEPSIESAQKLMTHKGIRLLAVTGGGAVVKAAMTSGKRAIA
                     AGPGNPPAVVDETARIDIAARGIIEGASIDNNIVCVAEKEICAVAKIADSLKAELVRQ
                     GCYLLNDRQVRDLEKVILDGDHVNKDWVGKNPSVIAKQIGVHVPDECRLLFAELPEQH
                     PFVQHELLMPVIGMVRYPDVASAIAGAVRCEHGFGHTAVMYSQNIEALSAMARTSNVS
                     IYVKNGSSLAGLGFGGEGYTSFTIASPTGEGMTTAVNFTRERRCTMKDAFRFV"
BASE COUNT          429 a          734 c          702 g          438 t
ORIGIN      
        1 gtcgtcctga ccaaaccgag ccgcggcaag tcaaaccagt aaccacccca cctgacaaaa
       61 ccccaaagcc gtcgcccagc caagccagtc accgttcaca taacggacgt gagcggtaca
      121 ttctggcgtg cggacaagct gcgcgcggtg cctactcgtc tgcggggcgg caggctacag
      181 cgtttgcaga aagtcggggt gcggacgcgc gatcagctcg accgaaagca aaagtccagg
      241 ggccaggagc tgttcgacag tcgcgagcgc cgcctgcacc atgtcctgtt caccgaccag
      301 gatcgcaaag ccttttccac ccagtccgtc gcacaaacga agatcgtgca aacggacttc
      361 ggcggctttg caggccgcat cggccccacg cagcgtcgat gccgcgctca gagtttccaa
      421 gatcccgatc gacaagtctt cgccaagcgg ggtcttgtct ttgcggcgca ggacatcgac
      481 cacttcgtcg tgaacttggg tgaggagcag ctcgtcacac agtgcggccc cagcttgcgc
      541 acgcccggct tccatcgctt cttcgacgga agcgacatcg ccgctgcaca acaccaggtg
      601 cttgcccggc gacactggac ggttcagatg aagctgcacc gcagcgcgct tgaccagcgc
      661 atcggcgacc gtcagaccgc gcgcgatcga ctgaagctca agaagaccca gcgccgcgcc
      721 ggtggcgacg atgtgttctg gctgacctgt gcgcagcgga cccaaacgaa ataactcccg
      781 tgccttaggc tacacgaaac ggaaggcgtc tttcatcgtg cagcgacgct cgcgggtgaa
      841 attgaccgca gtggtcatgc cctcgccggt cggcgaagca atggtaaacg acgtatagcc
      901 ttcgccacca aagcccaggc ccgccagact cgaaccattt ttgacgtaga tcgacacgtt
      961 ggatgtgcgc gccatggccg acagggcttc gatgttctgc gagtacatga cggcggtgtg
     1021 accaaagccg tgctcacagc ggacggcccc ggcaatcgcg gaagcgacat cggggtagcg
     1081 caccatgcca atcaccggca tcagcagttc atgctgcaca aacggatgct gctcgggcag
     1141 ctcggcaaac agcagccggc attcgtcggg gacatggacg ccgatctgtt tggcaatcac
     1201 cgagggattt ttgcccaccc agtctttgtt tacgtgatcg ccgtcgagaa tgactttttc
     1261 gaggtcgcga acttgccgat cgtttagcag atagcagccc tgccgcacga gctctgcctt
     1321 gaggctgtcg gcaatcttgg cgaccgcgca gatttctttt tctgcgacgc agacaatgtt
     1381 gttgtcgatc gatgcgcctt cgatgatgcc gcgggcggcg atgtcgatgc gggcggtttc
     1441 gtcgacgacg gcgggagggt tgcccggtcc agcggcaatc gcccgcttgc ccgaagtcat
     1501 cgcggctttg acgacagcgc caccacctgt gaccgcgagc aaccgaatcc ctttgtgggt
     1561 catcagcttt tgggcgcttt caatcgacgg ctccgccacg ctgcagagca ggttgcccgg
     1621 tccgcccgcc tgcacaatcg cttcgttgag gatgtggatg aaccaagcgc aggtccgcca
     1681 ggcatacgga tgggtgttga acaccacggc gttgccaccg gccagcatgc cgatcccgtt
     1741 gttcaggatc gtttcggtga cgttcgtcgt cggagtgatg gcgccgatga ccccgtaggc
     1801 cgcgcggtca agaacggtca gtccgtgatc gccgctaaag gccgtcggac ggaggatttc
     1861 cggccctggg gtcttcgtcg cgaccagcgt gttctttttg atcttgtcgt cgacacgccc
     1921 aagcccggtt tccgccaccg catactcggc gagcagcttg acgttgtcga gcgacacctt
     1981 gcgcatcgct gcgaccattt tttcgcgggt cgacagcggc gcggaagaat actgctcgaa
     2041 cgctttttgc gccgccgccg tcgccgagtc gatgtcttgg aacaggccgt gggtgccccg
     2101 cggaaccctg gcatccccgc gcttgccagc tccgcccccg cgaaggtgcg gcggcacaaa
     2161 cgcaccggga cgctgggacg tgccggtgtt tggcgtgcct ttgtccgccg ccttcatgac
     2221 cgcttcggcg ggcgtttgtt ggacgtcggg cgacaggcgc agaagcacct tgtcgacgat
     2281 ctcttggatg cgcttctcgt caa
//
LOCUS       DAORGN010000351         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_40206_length_2064_cov_6.333001, whole genome shotgun sequence.
ACCESSION   DAORGN010000351 DAORGN010000000
VERSION     DAORGN010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40206_length_2064_cov_6.333001"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PKL17_21530"
     CDS             complement(<1..1608)
                     /locus_tag="PKL17_21530"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNK47380.1"
                     /translation="MSRLRIQIVNPADGGFFYWLFKYYLFAALGLLMVSLHVLVAVYV
                     YFARTLPETPDLSRYAQLAPGITEVYSGDGSLLATLASERREILPLDKIPQSMLDAVI
                     AIEDRRFYEHRGLDLRGFVRAMRVNFYSGRMRQGGSTLTQQVAKAFLGADRTVSRKIR
                     EAILARRLEARYSKAEILQTYLNHTFLGHGSYGVQAAAKRFFDKNIWDLPVEDQAVLA
                     GLLKAPTRYSPIDHPQAAQQRRNLVLRKMVEVGKLDEATAAKLYGKPIVLQQRRDISR
                     DVSPYFVNHVRKELIATLGERRVMEDGLQVETTVQPFLDVLAGENVDFAVRRLDKRQG
                     YRGAEASLDPDAQRVFQLRAQKRYADPLKEDTLYLGLVTAVRPSGATVQIGPHTTELP
                     LNNMLWAARYNPRDPTNDRLILSAFEALKPGDVIWVKQAFRSNLAKFSDFVYAPITPY
                     STPEPKPPQAGQPPKPTPRPVVAAPAPAGERVQPVRTAAAAPPRTQAKPGTRPAGPAR
                     PGVATTRPGGRATQPGRGPGGRPTGSRP"
     gene            complement(1759..>2064)
                     /locus_tag="PKL17_21535"
     CDS             complement(1759..>2064)
                     /locus_tag="PKL17_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNK47381.1"
                     /translation="QQPLTYPLRFAIRFDEFDRQHKGMITFEVTALDSVGDALGQVQR
                     SVAINYTEKTAVSLSIGALFDMATPADLLPPPDLATPDMASVDAMPSDGPRPDGGPL"
BASE COUNT          375 a          634 c          654 g          401 t
ORIGIN      
        1 gggccggctt cccgtgggtc gcccaccagg tccgcgtccg ggctgcgtgg ctcggccacc
       61 gggtcgggtg gtcgcaacgc cgggtcttgc tggtcccgcc ggacgtgtgc ccggcttggc
      121 ttgtgtgcgt gggggcgcag cagccgcagt gcgaaccggc tgaacccgct ccccggctgg
      181 tgcaggcgcg gcgaccaccg gacgcggcgt cggtttgggc ggctgtcctg cctgtggcgg
      241 cttcggttcc ggtgtgctgt acggcgtaat cggcgcgtag acgaagtcac taaacttcgc
      301 aagattcgag cgaaacgcct gcttcaccca gatcacatcg cccggcttca gtgcttcaaa
      361 cgccgacaga atcagccgat cattggtcgg gtcgcgcggg ttatagcgcg ccgcccacag
      421 catgttgttg agcggcagct ccgttgtgtg cgggccaatt tgcaccgtcg cgcctgatgg
      481 acgcaccgcc gtcaccagcc cgaggtaaag cgtgtcttcc ttgagcgggt cagcgtagcg
      541 cttctgcgcc cgcaactgaa acacgcgctg ggcgtcggga tcgaggctcg cttcagcgcc
      601 gcgatagcct tgtcgtttgt cgagccggcg taccgcaaaa tcgacatttt cccctgccag
      661 cacatcgaga aatggctgca cggtcgtttc gacttgcagc ccgtcctcca tcacccgtcg
      721 ctcaccgagc gttgcgatca gctctttgcg gacgtgattg acgaaatacg gtgacacatc
      781 ccgcgaaatg tcccgtcgct gctggagcac gatcggcttg ccgtagagct tggccgccgt
      841 cgcttcatcg agcttgccga cttcgaccat tttgcgcagc accaagttgc gacgctgctg
      901 ggcggcttgc gggtggtcaa tcggtgaata gcgcgtcggt gctttgagca gtccggccag
      961 gactgcctga tcctcgacgg gcagatccca aatatttttg tcgaaaaaac gcttcgccgc
     1021 cgcttggaca ccgtagctgc catgtccgag gaaggtgtga ttgaggtagg tctgaaggat
     1081 ttccgcctta ctgtaacggg cttcgagccg tcgggccaag atcgcttcgc ggatcttgcg
     1141 tgagacggtc cgatcggcgc cgagaaacgc tttggcgacc tgctgggtca gcgtcgaacc
     1201 gccttgccgc atccggcccg agtaaaaatt gacccgcatc gcccgcacga agccgcgcag
     1261 gtcgagcccg cggtgttcgt agaagcggcg atcctcgatc gcgatcaccg catcgagcat
     1321 cgactgcgga atcttgtcga gcggcaggat ttctcgtcgt tcgctcgcca gtgtcgcaag
     1381 cagactgcca tcgcccgaat acacctcggt gataccgggg gccagctgtg catagcgcga
     1441 cagatccggc gtttcgggca gcgtccgcgc aaaatacacg tagaccgcca ccagcacatg
     1501 aagtgacacc atcagcagcc cgagggccgc gaacaggtag tacttgaaga gccagtagaa
     1561 gaacccaccg tcggcgggat tgacgatctg gatgcgcagt cgggacattc tttccgcgag
     1621 ctacccaaaa gccgtggcac tcgcccactc cctacgcacc ctatcacaaa actgaatgtt
     1681 ctttgaccct tggcgaggga ggagcttggc ctaccctggt aaagtaggcg atctaggctg
     1741 tggcaatggg gcgaaaggtt acaaggggcc tccgtcgggg cgcggtccgt cgcttggcat
     1801 ggcatcgacg gacgccatgt cgggggttgc caaatcgggc ggcggcaaca ggtcggcggg
     1861 cgtggccata tcaaacagcg cacctatcga cagcgatacc gccgtctttt cggtgtagtt
     1921 gatagccacg gaacgctgca cttgacccaa ggcgtctccg acggaatcga gcgccgtgac
     1981 ctcgaacgtg atcatgcctt tgtgttggcg atcaaactcg tcgaagcgga tcgcaaagcg
     2041 cagcggatag gtcagtggct gctg
//
LOCUS       DAORGN010000352         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Pseudomonadota bacterium isolate AMR_MDS_0801
            NODE_74327_length_1504_cov_4.726018, whole genome shotgun sequence.
ACCESSION   DAORGN010000352 DAORGN010000000
VERSION     DAORGN010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852144
            Sequence Read Archive: SRR3501886
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudomonadota bacterium (wastewater metagenome)
  ORGANISM  Pseudomonadota bacterium
            Bacteria; Pseudomonadota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:33:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,309
            CDSs (total)                      :: 4,272
            Genes (coding)                    :: 4,253
            CDSs (with protein)               :: 4,253
            Genes (RNA)                       :: 37
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 18 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Pseudomonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74327_length_1504_cov_4.726018"
                     /isolate="AMR_MDS_0801"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1977087"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKL17_21540"
     CDS             complement(<1..725)
                     /locus_tag="PKL17_21540"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNK47382.1"
                     /translation="MASSGLLASLSLDLDNQWSYMRTHGDAGWEAYPSYLHTVLPLAL
                     STVESVGLKMTVFLVGADAAMPQHRELFRSISERGHEVGNHSLHHLQWLHKLPHAELA
                     HEIVEADRLISEAAGQKPRGFRGPGFACSTTLLRILAQNGYDYDCSTFPTFVGPLARA
                     YYFMSARKLSDQESDERKDLFGSVRDGFRPLSGYLWALDERDSQGRPLPDLLEIPVTT
                     LPVLRTPIHMSYLLFLAQRSPFV"
     gene            866..>1504
                     /locus_tag="PKL17_21545"
     CDS             866..>1504
                     /locus_tag="PKL17_21545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNK47383.1"
                     /translation="MTGQTIGPYRVLREVGKGGMGAVYEAIHPQIERKVAIKVLHPEY
                     AVNQSVVQRFFNEARAVNIVNHPSVVQISEFGQQPDGTAYIVMEFLEGESLGARMKRF
                     GGKLSIADTLRLTRQVAAALAAAHAKNIVHRDLKPDNVMIVADPEAPGGERAKILDFG
                     IAKLASQTKDGEAAASQTSTGMMVGTPLYMSPEQCKGSGNITDKADVYSLGVM"
BASE COUNT          325 a          434 c          468 g          277 t
ORIGIN      
        1 atcacaaacg gcgatcgctg cgccaaaaac agcagatacg acatatgaat cggcgtgcgc
       61 aggaccggca gcgtcgtgac gggaatttcg agcagatcgg gaagcgggcg tccttgcgaa
      121 tcacgttcat cgagcgccca cagataaccg gacagcggtc ggaaaccgtc gcggacgcta
      181 ccaaacagat ccttgcgctc gtcggattcc tgatcgctta acttgcgcgc cgacataaag
      241 taatacgcgc gtgccagcgg cccgacgaac gtcggaaacg tcgagcagtc gtagtcgtag
      301 ccgttctgcg caaggatgcg caaaagcgtc gtactgcacg caaatccagg tccgcgaaaa
      361 ccgcgcggct tctgtcctgc cgcttcgctg atcaagcgat cggcctcgac gatttcatgg
      421 gcaagctcgg cgtgcggcag cttgtgcagc cactgcaagt ggtgtagcga gtggttgcct
      481 acttcgtggc cgcgctcgga aatcgaacga aacagctcgc gatgttgcgg catggcggcg
      541 tcggctccga cgagaaacac cgtcattttg agcccgaccg attccaccgt cgaaagcgcc
      601 agcggtagca ccgtgtgtag atacgacgga tacgcttccc agcccgcatc gccgtgcgtg
      661 cgcatgtacg accactggtt gtcgaggtcc aaagacagcg aggcgagcaa accagacgat
      721 gccatggcgg attcctttgc ggggtgagtg ggcaccggac attttcgcca aatgccaagc
      781 cggctgccag gttgtcggcc cgagagagag tttgtcaagc ttctctcaag gaaatttgtt
      841 atcttgatta cattaggtgg tgacgatgac cggacagact atcggtccct atcgcgtgct
      901 tcgcgaggtc ggcaaagggg ggatgggcgc ggtctatgag gcgattcacc cgcagatcga
      961 gcgcaaagtc gcaatcaagg ttttgcatcc cgagtatgcg gtgaatcagt ccgtcgtgca
     1021 gcgcttcttc aacgaggcgc gcgccgtcaa catcgtcaac catccgagcg tcgtccaaat
     1081 ttccgaattt ggccagcagc ccgacggcac ggcgtacatc gtgatggagt ttttggaagg
     1141 cgagtcgctc ggcgcgcgga tgaagcgctt tggcggcaag ctttcgatcg ctgacaccct
     1201 gcgactcacc cgccaagtag ccgccgccct cgccgccgcc cacgccaaaa acatcgttca
     1261 tcgtgacctg aagccggaca atgtgatgat tgtggcggat ccggaggcac cgggggggga
     1321 gcgggccaag atcctggact ttggcatcgc gaagcttgct tcgcagacca aggacggcga
     1381 agcggctgcg tcgcagacga gcaccgggat gatggtcgga acgccgcttt acatgtcgcc
     1441 cgagcagtgc aaaggcagcg gcaacatcac cgacaaggcc gatgtgtatt cgctcggggt
     1501 gatg
//
